WorldWideScience

Sample records for aps transport lines

  1. High beam current shut-off systems in the APS linac and low energy transfer line

    International Nuclear Information System (INIS)

    Two independent high beam current shut-off current monitoring systems (BESOCM) have been installed in the APS linac and the low energy transport line to provide personnel safety protection in the event of acceleration of excessive beam currents. Beam current is monitored by a fast current transformer (FCT) and fully redundant supervisory circuits connected to the Access Control Interlock System (ACIS) for beam intensity related shutdowns of the linac. One FCT is located at the end of the positron linac and the other in the low energy transport line, which directs beam to the positron accumulator ring (PAR). To ensure a high degree of reliability, both systems employ a continuous self-checking function, which injects a test pulse to a single-turn test winding after each ''real'' beam pulse to verify that the system is fully functional. The system is designed to be fail-safe for all possible system faults, such as loss of power, open or shorted signal or test cables, loss of external trigger, malfunction of gated integrator, etc. The system has been successfully commissioned and is now a reliable part of the total ACIS

  2. Proton-dependent glutamine uptake by aphid bacteriocyte amino acid transporter ApGLNT1.

    Science.gov (United States)

    Price, Daniel R G; Wilson, Alex C C; Luetje, Charles W

    2015-10-01

    Aphids house large populations of the gammaproteobacterial symbiont Buchnera aphidicola in specialized bacteriocyte cells. The combined biosynthetic capability of the holobiont (Acyrthosiphon pisum and Buchnera) is sufficient for biosynthesis of all twenty protein coding amino acids, including amino acids that animals alone cannot synthesize; and that are present at low concentrations in A. pisum's plant phloem sap diet. Collaborative holobiont amino acid biosynthesis depends on glutamine import into bacteriocytes, which serves as a nitrogen-rich amino donor for biosynthesis of other amino acids. Recently, we characterized A. pisum glutamine transporter 1 (ApGLNT1), a member of the amino acid/auxin permease family, as the dominant bacteriocyte plasma membrane glutamine transporter. Here we show ApGLNT1 to be structurally and functionally related to mammalian proton-dependent amino acid transporters (PATs 1-4). Using functional expression in Xenopus laevis oocytes, combined with two-electrode voltage clamp electrophysiology we demonstrate that ApGLNT1 is electrogenic and that glutamine induces large inward currents. ApGLNT1 glutamine induced currents are dependent on external glutamine concentration, proton (H+) gradient across the membrane, and membrane potential. Based on these transport properties, ApGLNT1-mediated glutamine uptake into A. pisum bacteriocytes can be regulated by changes in either proton gradients across the plasma membrane or membrane potential. PMID:26028424

  3. Analysis and Verification of Direct Vessel Injection Line Break event tree for AP1000 reactor with TRACE code

    Energy Technology Data Exchange (ETDEWEB)

    Queral, C.; Montero-Mayorga, J.; Gonzalez-Cadelo, J.

    2013-07-01

    The AP1000 PRA thermal hydraulic simulations were performed with MAAP code, which allows simulating sequences with low computational efforts. On the other hand, the use of best estimate codes allows verifying PRA results as well as obtaining a greater knowledge of the phenomenology of such sequences. The initiating event with the greatest contribution to core damage is Direct Vessel Injection Line Break (DVILB). This paper presents a review of DVILB sequences of AP1000 with TRACE code for verifying sequences previously analyzed by Westinghouse with MAAP code. The sequences which configure the DVILB event tree during short term have been simulated. The results obtained confirm the ones obtained in AP1000 PRA.

  4. Pulsational line profile variation of the roAp star HR 3831

    CERN Document Server

    Kochukhov, O

    2005-01-01

    We report the first comprehensive investigation of the line profile variation caused by non-radial pulsation in a magnetic oscillating chemically peculiar star. Spectrum variation of the well-known roAp star HR 3831 is detected using very high-resolution high signal-to-noise spectroscopic time-series observations and are followed through the whole rotation cycle of the star. We confirm outstanding diversity of pulsational behaviour of different lines in the HR 3831 spectrum and attribute this phenomenon to an interplay between extreme vertical chemical inhomogeneity of the HR 3831 atmosphere and a running pulsation wave, propagating towards the upper photospheric layers with increasing amplitude. Rapid profile variation of the NdIII 6145 A line is characterized by measuring changes of its equivalent width and the first three moments. We demonstrate that rotational modulation of the radial velocity oscillations cannot be fully explained by an oblique axisymmetric dipole (ell=1, m=0) mode, implied by the classi...

  5. Moisture Transport Through Sprayed Concrete Tunnel Linings

    Science.gov (United States)

    Holter, Karl Gunnar; Geving, Stig

    2016-01-01

    Waterproofing of permanent sprayed concrete tunnel linings with sprayed membranes in a continuous sandwich structure has been attempted since 2000 and has seen increased use in some countries. The main function of a sprayed membrane from a waterproofing perspective is to provide crack bridging and hence prevent flow of liquid water into the tunnel through cracks and imperfections in the concrete material. However, moisture can migrate through the concrete and EVA-based membrane materials by capillary and vapor diffusion mechanisms. These moisture transport mechanisms can have an influence on the degree of saturation, and may influence the pore pressures in the concrete material as well as risk of freeze-thaw damage of the concrete and membrane. The paper describes a detailed study of moisture transport material parameters, moisture condition in tunnel linings and climatic conditions tunnels in hard rock in Norway. These data have been included in a hygrothermal simulation model in the software WUFI for moisture transport to substantiate moisture transport and long-term effects on saturation of the concrete and membrane material. The findings suggest that EVA-based membranes exhibit significant water absorption and vapor transport properties although they are impermeable to liquid water flow. State-of-the-art sprayed concrete material applied with the wet mix method exhibits very low hydraulic conductivities, lower than 10-14 m/s, thus saturated conductive water flow is a very unlikely dominant transport mechanism. Moisture transport through the lining structure by capillary flow and vapor diffusion are calculated to approximately 3 cm3/m2 per day for lining thicknesses in the range of 25-35 cm and seasonal Nordic climate variations. The calculated moisture contents in the tunnel linings from the hygrothermal simulations are largely in agreement with the measured moisture contents in the tunnel linings. The findings also indicate that the concrete material exhibits

  6. Evidence for magnetar formation in broad-lined type Ic supernovae 1998bw and 2002ap

    CERN Document Server

    Wang, L J; Liu, L D; Wang, S Q; Han, Y H; Xu, D; Dai, Z G; Qiu, Y L; Wei, J Y

    2016-01-01

    Broad-lined type Ic supernovae (SNe Ic-BL) are peculiar stellar explosions that distinguish themselves from ordinary SNe. Some SNe Ic-BL are associated with long-duration (\\gtrsim 2 s) gamma-ray bursts (GRBs). Black holes and magnetars are two types of compact objects that are hypothesized to be central engines of GRBs. In spite of decades of investigations, no direct evidence for the formation of black holes or magnetars has been found for GRBs so far. Here we report the discovery that the early peak (t \\lesssim 50 days) and late-time (t \\gtrsim 300 days) slow decay displayed in the light curves of both SNe 1998bw (associated with GRB 980425) and 2002ap (not GRB-associated) can be attributed to magnetar spin-down with initial rotation period P0 \\sim 20 ms, while the intermediate-time (50 \\lesssim t \\lesssim 300 days) linear decline is caused by radioactive decay of 56Ni. The connection between the early peak and late-time slow decline in the light curves is unexpected in alternative models. We thus suggest t...

  7. Chloride transport in a glioma cell line

    Energy Technology Data Exchange (ETDEWEB)

    Wolpaw, E.W.

    1984-01-01

    Maintenance of the extracellular environment is a major function of central nervous system astroglia. The transport of Cl/sup -/ across the cell membrane may be an integral part of this function, since Cl/sup -/ transport has been implicated in homeostasis of cell volume, pH, and extracellular K/sup +/ concentration. The work presented here investigated Cl/sup -/ transport in the glioma cell line LRM55. Results indicate that LRM55 cells are a good model for astroglia and that these cells contain three Cl/sup -/ transporters; a Cl/sup -//HCO/sub 3//sup -/ exchanger, a K/sup +//Cl/sup -/ cotransporter, and a Cl/sup -//SO/sub 4//sup 2 -/ exchanger. Ion transport studies measured the fluxes of Cl/sup -/ (as /sup 36/Cl/sup -/), K/sup +/ (as /sup 86/Rb/sup +/), and SO/sub 4//sup 2 -/ (as /sup 35/SO/sub 4//sup 2 -/). Cl/sup -/ flux was trans-simulated by Cl/sup -/ or HCO/sub 3//sup -/ and was inhibited by SITS or furosemide. External K/sup +/ stimulated Cl/sup -/ influx and external Cl/sup -/ stimulated Rb/sup +/ influx. Furosemide, but not SITS, inhibited the K/sup +//Cl/sup -/ cotransporter. High K/sup +/ medium increased cell volume and Cl/sup -/ content. Steady-state Cl/sup -/ concentration was at least twice that predicted from passive equilibration according to the Nernst equation. SO/sub 4//sup 2 -/ flux was trans-stimulated by SO/sub 4//sup 2 -/ or by Cl/sup -/. Cl/sup -/ was a competitive inhibitor of SO/sub 4//sup 2 -/ influx, but SO/sub 4//sup 2 -/ had no detectable effect on Cl/sup -/ influx or efflux. SO/sub 4//sup 2 -/ flux was inhibited by SITS or furosemide.

  8. Development and evaluation of human AP endonuclease inhibitors in melanoma and glioma cell lines

    DEFF Research Database (Denmark)

    Mohammed, M Z; Vyjayanti, V N; Laughton, C A;

    2011-01-01

    Modulation of DNA base excision repair (BER) has the potential to enhance response to chemotherapy and improve outcomes in tumours such as melanoma and glioma. APE1, a critical protein in BER that processes potentially cytotoxic abasic sites (AP sites), is a promising new target in cancer...

  9. Intracellular transport of MHC class II and associated invariant chain in antigen presenting cells from AP-3-deficient mocha mice.

    Science.gov (United States)

    Sevilla, L M; Richter, S S; Miller, J

    2001-06-15

    MHC class II-restricted antigen presentation requires trafficking of newly synthesized class II-invariant chain complexes from the trans-Golgi network to endosomal, peptide-loading compartments. This transport is mediated by dileucine-like motifs within the cytosolic tail of the invariant chain. Although these signals have been well characterized, the cytosolic proteins that interact with these dileucine signals and mediate Golgi sorting and endosomal transport have not been identified. Recently, an adaptor complex, AP-3, has been identified that interacts with dileucine motifs and mediates endosomal/lysosomal transport in yeast, Drosophila, and mammals. In this report, we have assessed class II-invariant chain trafficking in a strain of mice (mocha) which lacks expression of AP-3. Our studies demonstrate that the lack of AP-3 does not affect the kinetics of invariant chain degradation, the route of class II-invariant chain transport, or the rate and extent of class II-peptide binding as assessed by the generation of SDS-stable dimers. The possible role of other known or unknown adaptor complexes in class II-invariant chain transport is discussed. PMID:11520080

  10. Breeding of Selectable Marker-Free Transgenic Rice Lines Containing AP1 Gene with Enhanced Disease Resistance

    Institute of Scientific and Technical Information of China (English)

    YU Heng-xiu; LIU Qiao-quan; WANG Ling; ZHAO Zhi-peng; XU Li; HUANG Ben-li; GONG Zhi-yun; TANG Shu-zhu; GU Ming-hong

    2006-01-01

    In order to obtain marker-free transgenic rice with improved disease resistance, the AP1 gene of Capsicum annuum and hygromycin-resistance gene (HPT) were cloned into the two separate T-DNA regions of the binary vector pSB 130,respectively, and introduced into the calli derived from the immature seeds of two elite japonica rice varieties, Guangling Xiangjing and Wuxiangjing 9, mediated by Agrobacterium-mediated transformation. Many cotransgenic rice lines containing both the AP1 gene and the marker gene were regenerated and the integration of both transgenes in the transgenic rice plants was confirmed by either PCR or Southern blotting technique. Several selectable marker-free transgenic rice plants were subsequently obtained from the progeny of the cotransformants, and confirmed by both PCR and Southern blotting analysis. These transgenic rice lines were tested in the field and their resistance to disease was c arefully investigated, the results showed that after inoculation the resistance to either bacterial blight or sheath blight of the selected transgenic lines was improved when compared with those of wild type.

  11. Interaction Between The Broad-lined Type Ic Supernova 2012ap and Carriers of Diffuse Interstellar Bands

    CERN Document Server

    Milisavljevic, D; Crabtree, K N; Foster, J B; Soderberg, A M; Fesen, R A; Parrent, J T; Sanders, N E; Drout, M R; Kamble, A; Chakraborti, S; Pickering, T E; Cenko, S B; Silverman, J M; Filippenko, A V; Kirshner, R P; Mazzali, P; Maeda, K; Marion, G H; Vinko, J; Wheeler, J C

    2014-01-01

    The diffuse interstellar bands (DIBs) are absorption features observed in optical and near-infrared spectra that are thought to be associated with carbon-rich polyatomic molecules in interstellar gas. However, because the central wavelengths of these bands do not correspond with electronic transitions of any known atomic or molecular species, their nature has remained uncertain since their discovery almost a century ago. Here we report on unusually strong DIBs in optical spectra of the broad-lined Type Ic supernova SN 2012ap that exhibit changes in equivalent width over short (~30 days) timescales. The 4428 and 6283 Angstrom DIB features get weaker with time, whereas the 5780 Angstrom feature shows a marginal increase. These nonuniform changes suggest that the supernova is interacting with a nearby source of the DIBs and that the DIB carriers possess high ionization potentials, such as small cations or charged fullerenes. We conclude that moderate-resolution spectra of supernovae with DIB absorptions obtained...

  12. AP1000控制棒驱动线落棒试验研究%Study on Drop Performance Test of AP1000 Control Rod Drive Line

    Institute of Scientific and Technical Information of China (English)

    顾汉洋; 张朝柱; 陈宇清; 周肖佳; 刘刚

    2015-01-01

    采用1组全尺寸AP1000控制棒驱动线进行落棒试验,研究不同纵向流和横向流以及错位和变形对落棒时间的影响,并用高速摄像图像处理技术获得落棒行程速度曲线.试验结果表明,纵向流量是影响驱动线落棒时间的主要因素;在本试验范围内横向流以及部件的错位变形对AP1000控制棒驱动线落棒时间的影响都较为有限.

  13. Automatically operated maglev public transport line in Nagoya

    Energy Technology Data Exchange (ETDEWEB)

    Hibi, Osamu [Aichi Rapid Transit Co., Aichi Prefecture (Japan). Engineering Dept.

    2006-07-01

    Tobu-Kyuryo-Line in Nagoya (Japan) is a mid-size automatically operated passenger line which adopts the HSST (high-speed surface transport) system. The vehicles are levitated with normal conducting electromagnets and propelled by a linear induction motor. Low noise by levitation and stable acceleration by the linear induction motor improve the riding comfort and ensure a faster transportation. Automatic operation is indispensable for stable operation and reduction of running costs. In the opening year, the Tobu-Kyuryo-Line played an important role as an access to EXPO2005 by carrying 20 million passengers. So far it had no serious trouble and has been operated safely. (orig.)

  14. SPES-2, the full-height, full-pressure test facility simulating the AP600 plant comparison among 2' small break tests located on different lines

    International Nuclear Information System (INIS)

    SPES-2 is a full height, full pressure experimental test facility reproducing the Westinghouse AP600 reactor with a scaling factor of 1/395. The experimental plant, designed and operated by SIET in Piacenza, consists of a full simulation of the AP600 primary core cooling system including all the passive and active safety systems. In 1992, Westinghouse, in cooperation with ENEL, ENEA, SIET and ANSALDO developed an experimental program to test the integrated behaviour of the AP600 passive safety systems. The SPES-2 test matrix, concluded in November'94, has examined the AP600 passive safety system response for a range of small break LOCAs at different locations on the primary system and on the passive system lines; single steam generator tube ruptures with both passive and active non-safety systems, and a main steam line break transient to demonstrate the capability of passive safety systems for rapid cooldown. Each of the tests has provided detailed experimental results for verification of the capability of the analysis methods to predict the integrated passive safety system behaviour. The author will describe the results obtained during experimental test facility having the same break size (2 inches) but located in different plant positions (cold leg, Direct Vessel Injection line, cold leg-CMT balance line) in order to determine the effect of break location on the plant behaviour

  15. Comparison of curved trajectory atmospheric transport vs. assumed straight-line transport

    International Nuclear Information System (INIS)

    The Dose-To-Man program as currently being implemented at the Savannah River Laboratory provides estimates of the environmental effects of Savannah River Plant operations. These estimates utilize a polar grid structure and assume straight-line transport of materials released to the atmosphere to distances beyond grid boundaries. The assumption of straight-line transport is standard for such estimates and greatly simplifies the computations involved in providing estimates of effects. Generally, the atmospheric transport trajectory will be curved; and, for distances out to 100 km or so, the assumption may lead to significant error. Therefore, a computer code was constructed to provide an estimate of the curved trajectory transport relative to straight-line transport over an extended time period

  16. Design of the SLC damping ring to linac transport lines

    International Nuclear Information System (INIS)

    The first and second order optics for the damping ring to linac transport line are designed to preserve the damped transverse emittance while simultaneously compressing the bunch length of the beam to that length required for reinjection into the linac. This design, including provisions for future control of beam polarization, is described

  17. Transportation characteristics of nolatrexed in three tumor cell lines

    Institute of Scientific and Technical Information of China (English)

    LI Yi-lei; ZHAO Ai-guo; WU Shu-guang

    2002-01-01

    Objective:To investigate the association of the transportation characteristics of nolatrexed in tumor cells with its drug sensitivity. Methods: The sensitivity of 3 tumor cell lines, C6, SRS82 and LoVo, to nolatrexed were determined by growth inhibition study. After exposure to 20 μmol/L nolatrexed at different time intervals ranging from 0 to 30 min, or to nolatrexed at different concentrations ranging from 0 to 40μmol/L for 10 min, the intracellular drug concentration was measured using high-performance liquid chromatography. Results: C6 was the most sensitive cell line among the three, with sensitivity 6. 8-fold and 13.8-fold those of SRS-82 and LoVo cells respectively. Transportation of nolatrexed in the 3 cell lines were qualitatively similar, which rapidly achieved steady-state within 5 min, and linear relationship between the intracellular and extracellular drug concentration was observed. The intracellular steady-state level achieved in C6 was significantly higher than those in the other two cell lines, the latter having comparable levels. Conclusion: Nolatrexed enters the cell very quickly and different transport capacities are involved in the generation of varied sensitivity to nolatrexed in tumor cells.

  18. SPES-2, AP600 intergral system test S01007 2 inch CL to core make-up tank pressure balance line break

    Energy Technology Data Exchange (ETDEWEB)

    Bacchiani, M.; Medich, C.; Rigamonti, M. [SIET S.p.A. Piacenza (Italy)] [and others

    1995-09-01

    The SPES-2 is a full height, full pressure experimental test facility reproducing the Westinghouse AP600 reactor with a scaling factor of 1/395. The experimental plant, designed and operated by SIET in Piacenza, consists of a full simulation of the AP600 primary core cooling system including all the passive and active safety systems. In 1992, Westinghouse, in cooperation with ENEL (Ente Nazionale per l` Energia Elettrica), ENEA (Enter per le numove Technlogie, l` Energia e l` Ambient), Siet (Societa Informazioni Esperienze Termoidraulich) and ANSALDO developed an experimental program to test the integrated behaviour of the AP600 passive safety systems. The SPES-2 test matrix, concluded in November 1994, has examined the AP600 passive safety system response for a range of small break LOCAs at different locations on the primary system and on the passive system lines; single steam generator tube ruptures with passive and active safety systems and a main steam line break transient to demonstrate the boration capability of passive safety systems for rapid cooldown. Each of the tests has provided detailed experimental results for verification of the capability of the analysis methods to predict the integrated passive safety system behaviour. Cold and hot shakedown tests have been performed on the facility to check the characteristics of the plant before starting the experimental campaign. The paper first presents a description of the SPES-2 test facility then the main results of S01007 test {open_quotes}2{close_quotes} Cold Leg (CL) to Core Make-up Tank (CMT) pressure balance line break{close_quotes} are reported and compared with predictions performed using RELAP5/mod3/80 obtained by ANSALDO through agreement with U.S.N.R.C. (U.S. Nuclear Regulatory Commission). The SPES-2 nodalization and all the calculations here presented were performed by ANSALDO and sponsored by ENEL as a part of pre-test predictions for SPES-2.

  19. Mutation in AP-3 delta in the mocha mouse links endosomal transport to storage deficiency in platelets, melanosomes, and synaptic vesicles.

    Science.gov (United States)

    Kantheti, P; Qiao, X; Diaz, M E; Peden, A A; Meyer, G E; Carskadon, S L; Kapfhamer, D; Sufalko, D; Robinson, M S; Noebels, J L; Burmeister, M

    1998-07-01

    The mouse mutant mocha, a model for the Hermansky-Pudlak storage pool deficiency syndrome, is characterized by defective platelets, coat and eye color dilution, lysosomal abnormalities, inner ear degeneration, and neurological deficits. Here, we show that mocha is a null allele of the delta subunit of the adaptor-like protein complex AP-3, which is associated with coated vesicles budding from the trans-Golgi network, and that AP-3 is missing in mocha tissues. In mocha brain, the ZnT-3 transporter is reduced, resulting in a lack of zinc-associated Timm historeactivity in hippocampal mossy fibers. Our results demonstrate that the AP-3 complex is responsible for cargo selection to lysosome-related organelles such as melanosomes and platelet dense granules as well as to neurotransmitter vesicles. PMID:9697856

  20. Phenomena identification and ranking tables for Westinghouse AP600 small break loss-of-coolant accident, main steam line break, and steam generator tube rupture scenarios

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, G.E.; Fletcher, C.D.; Davis, C.B. [and others

    1997-06-01

    This report revision incorporates new experimental evidence regarding AP600 behavior during small break loss-of-coolant accidents. This report documents the results of Phenomena Identification and Ranking Table (PIRT) efforts for the Westinghouse AP600 reactor. The purpose of this PIRT is to identify important phenomena so that they may be addressed in both the experimental programs and the RELAP5/MOD3 systems analysis computer code. In Revision of this report, the responses of AP600 during small break loss-of-coolant accident, main steam line break, and steam generator tube rupture accident scenarios were evaluated by a committee of thermal-hydraulic experts. Committee membership included Idaho National Engineering and Environmental Laboratory staff and recognized thermal-hydraulic experts from outside of the laboratory. Each of the accident scenarios was subdivided into separate, sequential periods or phases. Within each phase, the plant behavior is controlled by, at most, a few thermal-hydraulic processes. The committee identified the phenomena influencing those processes, and ranked & influences as being of high, medium, low, or insignificant importance. The primary product of this effort is a series of tables, one for each phase of each accident scenario, describing the thermal-hydraulic phenomena judged by the committee to be important, and the relative ranking of that importance. The rationales for the phenomena selected and their rankings are provided. This document issue incorporates an update of the small break loss-of-coolant accident portion of the report. This revision is the result of the release of experimental evidence from AP600-related integral test facilities (ROSA/AP600, OSU, and SPES) and thermal-hydraulic expert review. The activities associated with this update were performed during the period from June 1995 through November 1996. 8 refs., 26 figs., 42 tabs.

  1. Phenomena identification and ranking tables for Westinghouse AP600 small break loss-of-coolant accident, main steam line break, and steam generator tube rupture scenarios

    International Nuclear Information System (INIS)

    This report revision incorporates new experimental evidence regarding AP600 behavior during small break loss-of-coolant accidents. This report documents the results of Phenomena Identification and Ranking Table (PIRT) efforts for the Westinghouse AP600 reactor. The purpose of this PIRT is to identify important phenomena so that they may be addressed in both the experimental programs and the RELAP5/MOD3 systems analysis computer code. In Revision of this report, the responses of AP600 during small break loss-of-coolant accident, main steam line break, and steam generator tube rupture accident scenarios were evaluated by a committee of thermal-hydraulic experts. Committee membership included Idaho National Engineering and Environmental Laboratory staff and recognized thermal-hydraulic experts from outside of the laboratory. Each of the accident scenarios was subdivided into separate, sequential periods or phases. Within each phase, the plant behavior is controlled by, at most, a few thermal-hydraulic processes. The committee identified the phenomena influencing those processes, and ranked ampersand influences as being of high, medium, low, or insignificant importance. The primary product of this effort is a series of tables, one for each phase of each accident scenario, describing the thermal-hydraulic phenomena judged by the committee to be important, and the relative ranking of that importance. The rationales for the phenomena selected and their rankings are provided. This document issue incorporates an update of the small break loss-of-coolant accident portion of the report. This revision is the result of the release of experimental evidence from AP600-related integral test facilities (ROSA/AP600, OSU, and SPES) and thermal-hydraulic expert review. The activities associated with this update were performed during the period from June 1995 through November 1996. 8 refs., 26 figs., 42 tabs

  2. Bending magnets for the CBA beam-transport line

    Energy Technology Data Exchange (ETDEWEB)

    Thern, R.E.

    1983-01-01

    The beam-transport line from the AGS to CBA requires 68 large bending magnets, consisting of pure dipoles and two types of combined function gradient magnets. All three types were designed with magnetic-field calculation program POISSON, using the same exterior dimensions and coil package. The design goal of +-1% momentum acceptance for the transport line required a wide horizontal aperture, with a much-smaller vertical aperture for economy. Two prototypes of one gradient magnet were built, and a facility constructed to measure them and the later production magnets. Measurements were done using both a long coil and a point coil (Rawson-Lush gaussmeter). Preliminary results show ..delta..B/B < 0.2 x 10/sup -3/, ..delta..G/G < 0.3 x 10/sup -2/, and ..delta..B/sub 2//B < 0.3 x 10/sup -4/ cm/sup -2/ over the beam aperture. Due to end effects, the actual gradient differs from the design gradient by 1%, which has been compensated for in the beam-line design.

  3. New magnet transport system for the LHC beam transfer lines

    CERN Multimedia

    Patrice Loïez

    2003-01-01

    The first of 700 magnets has been installed in one of the two transfer tunnels built to transfer the SPS beam into the LHC. The start of this first installation phase of the LHC transfer lines provides the opportunity to launch a new and highly original modular system for transporting and installing all kinds of magnets in very narrow tunnels. The system (pictured here in one of the tunnels) is based on very compact bogies, up to four of which can be coupled together to form a convoy. The wheels are fitted with individual motors enabling them to swivel through an angle of 90° and the convoy to move laterally. The lead vehicle is powered by an electric rail set into the roof of the tunnel. The system is backed up by electrical batteries that enable it to operate in the absence of an outside power source or in the event of power failure. Last but not least, for the long-distance transport of magnets, it can be optically guided by a line traced on the tunnel floor. The convoy moves through the particularly narr...

  4. Development of new transport line (TL-1) for Indus facility

    International Nuclear Information System (INIS)

    Transport Line-1 (TL-1) is a part of pre-injector system of Synchrotron Radiation Sources Indus-1 and Indus-2. In order to insert more number of diagnostic devices, especially Beam Slit Monitors for observing the beam position at various locations on TL-1, and to study the angular divergence etc., one new UHV system for TL-1 was designed and developed. Total length of TL-1 is ∼ 13.6 metres. This line transports the electron beam from the Microtron to the Booster Synchrotron. TL-1 comprises of Stainless Steel (SS grade: AISI 316) chambers, with ports for pumps, gauges, bellows for mechanical flexibility, isolation valves, and different Beam Diagnostic Devices like Beam Slit Monitors (BSM), Beam Profile Monitors (BPM), Secondary Emission Wire Monitors (SEWM) and Fast Current Transformers (FCT). All the associated UHV components were fabricated and qualified for UHV, individually in the UHV Lab. Vacuum in the range of 10-9-10-8 mbar was achieved. Ultimately all the components, (including the Beam Diagnostic Devices), are assembled in a mock assembly as per their original positions and tested. The aim of this exercise is to resolve the various unforeseen minor problems, in assembly, well in advance, so that the final assembly in actual position can be completed within minimum time period, (minimum down period of Indus facility), with more efficiency. This paper describes the testing procedures and the results of this exercise. (author)

  5. The new control system of HLS linac and transport line

    Institute of Scientific and Technical Information of China (English)

    LIU Gong-Fa; LI Wei-Min; LI Jing-Yi; LI Chuan; CHEN Li-Ping; BAO Xun; WANG Ji-Gang; XUAN Ke

    2005-01-01

    The new linac and transport line control system of Hefei Light Source (HLS) is a distributed control system based on EPICS (Experimental Physics and Industrial Control System). Industrial PC (IPC) is widely used as not only Input/Output Controller (IOC) but also device controller. Besides industrial PC, PLC and microcontroller are also used as device controllers. The software for industrial PC based device controller is developed based on VxWorks real-time operating system. The software for PLC and microcontroller are written with ladder software package and assemble language, respectively. PC with Linux and SUN workstation with Solaris are used as operator interfaces (OPI). High level control is made up of some EPICS tools and Tcl/Tk scripts.

  6. Radiation protection considerations along a radioactive ion beam transport line

    Science.gov (United States)

    Sarchiapone, Lucia; Zafiropoulos, Demetre

    2016-09-01

    The goal of the SPES project is to produce accelerated radioactive ion beams for Physics studies at “Laboratori Nazionali di Legnaro” (INFN, Italy). This accelerator complex is scheduled to be built by 2016 for an effective operation in 2017. Radioactive species are produced in a uranium carbide target, by the interaction of 200 μA of protons at 40 MeV. All of the ionized species in the 1+ state come out of the target (ISOL method), and pass through a Wien filter for a first selection and an HMRS (high mass resolution spectrometer). Then they are transported by an electrostatic beam line toward a charge state breeder (where the 1+ to n+ multi-ionization takes place) before selection and reacceleration at the already existing superconducting linac. The work concerning dose evaluations, activation calculation, and radiation protection constraints related to the transport of the radioactive ion beam (RIB) from the target to the mass separator will be described in this paper. The FLUKA code has been used as tool for those calculations needing Monte Carlo simulations, in particular for the evaluation of the dose rate due to the presence of the radioactive beam in the selection/interaction points. The time evolution of a radionuclide inventory can be computed online with FLUKA for arbitrary irradiation profiles and decay times. The activity evolution is analytically evaluated through the implementation of the Bateman equations. Furthermore, the generation and transport of decay radiation (limited to gamma, beta- and beta+ emissions) is possible, referring to a dedicated database of decay emissions using mostly information obtained from NNDC, sometimes supplemented with other data and checked for consistency. When the use of Monte Carlo simulations was not feasible, the Bateman equations, or possible simplifications, have been used directly.

  7. Observation and analysis of self-amplified spontaneous emission at the APS low-energy undulator test line

    CERN Document Server

    Arnold, N D; Banks, G; Bechtold, R; Beczek, K; Benson, C; Berg, S; Berg, W; Biedron, S G; Biggs, J A; Boerste, K; Borland, M; Bosek, M; Brzowski, W R; Budz, J; Carwardine, J A; Castro, P; Chae, Y C; Christensen, S; Clark, C; Conde, M; Crosbie, E A; Decker, G A; Dejus, Roger J; Deleon, H; Den Hartog, P K; Deriy, B N; Dohan, D; Dombrowski, P; Donkers, D; Doose, C L; Dortwegt, R J; Edwards, G A; Eidelman, Y; Erdmann, M J; Error, J J; Ferry, R; Flood, R; Forrestal, J; Freund, H; Friedsam, H; Gagliano, J; Gai, W; Galayda, J N; Gerig, R; Gilmore, R L; Gluskin, E; Goeppner, G A; Goetzen, J; Gold, C; Grelick, A E; Hahne, M W; Hanuska, S; Harkay, K C; Harris, G; Hillman, A L; Hogrefe, R; Hoyt, J; Huang, Z; Jagger, J M; Jansma, W G; Jaski, M; Jones, S J; Keane, R T; Kelly, A L; Keyser, C; Kim, K J; Kim, S H; Kirshenbaum, M; Klick, J H; Knoerzer, K; Knott, M; Koldenhoven, R J; Labuda, S; Laird, R; Lang, J; Lenkszus, F R; Lessner, E S; Lewellen, J W; Li, Y; Lill, R M; Lumpkin, Alex H; Makarov, O A; Markovich, G M; McDowell, M; McDowell, W P; McNamara, P E; Meier, T; Meyer, D; Michalek, W; Milton, S V; Moe, H; Moog, E; Morrison, L; Nassiri, A; Noonan, J R; Otto, R; Pace, J; Pasky, S J; Penicka, J M; Pietryla, A F; Pile, G; Pitts, C; Power, J; Powers, T; Putnam, C C; Puttkammer, A J; Reigle, D; Reigle, L; Ronzhin, D; Rotela, E R; Russell, E F; Sajaev, Vadim; Sarkar, S; Scapino, J C; Schröder, K; Seglem, R A; Sereno, N S; Sharma, S K; Sidarous, J F; Singh, O; Smith, T L; Soliday, R; Sprau, G A; Stein, S J; Stejskal, B; Svirtun, V; Teng, L C; Theres, E; Thompson, K; Tieman, B J; Torres, J A; Trakhtenberg, E; Travish, G; Trento, G F; Vacca, J; Vasserman, I B; Vinokurov, N A; Walters, D R; Wang, J; Wang, X J; Warren, J; Wesling, S; Weyer, D L; Wiemerslage, G; Wilhelmi, K; Wright, R; Wyncott, D; Xu, S; Yang, B X; Yoder, W; Zabel, R B

    2001-01-01

    Exponential growth of self-amplified spontaneous emission at 530 nm was first experimentally observed at the Advanced Photon Source low-energy undulator test line in December 1999. Since then, further detailed measurements and analysis of the results have been made. Here, we present the measurements and compare these with calculations based on measured electron beam properties and theoretical expectations.

  8. Observation and analysis of self-amplified spontaneous emission at the APS low-energy undulator test line

    Energy Technology Data Exchange (ETDEWEB)

    Arnold, N.D.; Attig, J.; Banks, G.; Bechtold, R.; Beczek, K.; Benson, C.; Berg, S.; Berg, W.; Biedron, S.G.; Biggs, J.A.; Borland, M.; Boerste, K.; Bosek, M.; Brzowski, W.R.; Budz, J.; Carwardine, J.A.; Castro, P.; Chae, Y.-C.; Christensen, S.; Clark, C.; Conde, M.; Crosbie, E.A.; Decker, G.A.; Dejus, R.J.; DeLeon, H.; Den Hartog, P.K.; Deriy, B.N.; Dohan, D.; Dombrowski, P.; Donkers, D.; Doose, C.L.; Dortwegt, R.J.; Edwards, G.A.; Eidelman, Y.; Erdmann, M.J.; Error, J.; Ferry, R.; Flood, R.; Forrestal, J.; Freund, H.; Friedsam, H.; Gagliano, J.; Gai, W.; Galayda, J.N.; Gerig, R.; Gilmore, R.L.; Gluskin, E.; Goeppner, G.A.; Goetzen, J.; Gold, C.; Gorski, A.J.; Grelick, A.E.; Hahne, M.W.; Hanuska, S.; Harkay, K.C.; Harris, G.; Hillman, A.L.; Hogrefe, R.; Hoyt, J.; Huang, Z.; Jagger, J.M.; Jansma, W.G.; Jaski, M.; Jones, S.J.; Keane, R.T.; Kelly, A.L.; Keyser, C.; Kim, K.-J.K.-J.; Kim, S.H.; Kirshenbaum, M.; Klick, J.H.; Knoerzer, K.; Koldenhoven, R.J.; Knott, M.; Labuda, S.; Laird, R.; Lang, J.; Lenkszus, F.; Lessner, E.S.; Lewellen, J.W.; Li, Y.; Lill, R.M.; Lumpkin, A.H.; Makarov, O.A.; Markovich, G.M.; McDowell, M.; McDowell, W.P.; McNamara, P.E.; Meier, T.; Meyer, D.; Michalek, W.; Milton, S.V. E-mail: milton@aps.anl.gov; Moe, H.; Moog, E.R.; Morrison, L.; Nassiri, A.; Noonan, J.R.; Otto, R.; Pace, J.; Pasky, S.J.; Penicka, J.M.; Pietryla, A.F.; Pile, G.; Pitts, C.; Power, J.; Powers, T.; Putnam, C.C.; Puttkammer, A.J.; Reigle, D.; Reigle, L.; Ronzhin, D.; Rotela, E.R.; Russell, E.F.; Sajaev, V.; Sarkar, S.; Scapino, J.C.; Schroeder, K.; Seglem, R.A.; Sereno, N.S.; Sharma, S.K.; Sidarous, J.F.; Singh, O.; Smith, T.L.; Soliday, R.; Sprau, G.A.; Stein, S.J.; Stejskal, B.; Svirtun, V.; Teng, L.C.; Theres, E.; Thompson, K.; Tieman, B.J.; Torres, J.A.; Trakhtenberg, E.M.; Travish, G.; Trento, G.F.; Vacca, J.; Vasserman, I.B.; Vinokurov, N.A.; Walters, D.R.; Wang, J.; Wang, X.J.; Warren, J.; Wesling, S.; Weyer, D.L.; Wiemerslage, G.; Wilhelmi, K.; Wright, R.

    2001-12-21

    Exponential growth of self-amplified spontaneous emission at 530 nm was first experimentally observed at the Advanced Photon Source low-energy undulator test line in December 1999. Since then, further detailed measurements and analysis of the results have been made. Here, we present the measurements and compare these with calculations based on measured electron beam properties and theoretical expectations.

  9. The Broad-Lined Type Ic SN 2012ap and the Nature of Relativistic Supernovae Lacking a Gamma-ray Burst Detection

    CERN Document Server

    Milisavljevic, D; Parrent, J T; Soderberg, A M; Fesen, R A; Mazzali, P; Maeda, K; Sanders, N E; Cenko, S B; Silverman, J M; Filippenko, A V; Kamble, A; Chakraborti, S; Drout, M R; Kirshner, R P; Pickering, T E; Kawabata, K; Hattori, T; Hsiao, E Y; Stritzinger, M D; Marion, G H; Vinko, J; Wheeler, J C

    2014-01-01

    We present ultraviolet, optical, and near-infrared observations of SN 2012ap, a broad-lined Type Ic supernova in the galaxy NGC 1729 that produced a relativistic and rapidly decelerating outflow without a gamma-ray burst signature. Photometry and spectroscopy follow the flux evolution from -13 to +272 days past the B-band maximum of -17.4 +/- 0.5 mag. The spectra are dominated by Fe II, O I, and Ca II absorption lines at ejecta velocities of 20,000 km/s that change slowly over time. Other spectral absorption lines are consistent with contributions from photospheric He I, and hydrogen may also be present at higher velocities (> 27,000 km/s). We use these observations to estimate explosion properties and derive a total ejecta mass of 2.7 Msolar, a kinetic energy of 1.0x10^{52} erg, and a 56Ni mass of 0.1-0.2 Msolar. Nebular spectra (t > 200d) exhibit an asymmetric double-peaked [OI] 6300,6364 emission profile that we associate with absorption in the supernova interior, although toroidal ejecta geometry is an al...

  10. CSR effect at XFEL-to-storage ring beam transport line, SPring-8

    International Nuclear Information System (INIS)

    A 300-meter long beam transport line will be built from the XFEL C-band linac to the existing 8 GeV storage ring at the SPring-8. The beam transport line is supposed to deliver femtosecond electron bunches with high peak current, being composed of 14 bending magnets and quadrupoles. Current status of the transport line construction plan is briefly presented. Since Coherent Synchrotron Radiation (CSR) could degrade a beam quality in such a transport line with multiple bends, tracking simulation on the effect has been employed to analyze the effect in the transport line. As a result, it was found that the CSR effect would not be negligible unless some modification would be made to either an electron bunch distribution or a lattice of the beam transport line. The numerical result on the CSR effect as well as a proposal to compress femtosecond bunches by positively utilizing CSR effect is shown. (author)

  11. Erratum: ``The Pure Rotational Line Emission of Ortho-Water Vapor in Comets. I. Radiative Transfer Model'' (ApJ, 615, 531 [2004])

    Science.gov (United States)

    Bensch, F.; Bergin, E. A.

    2007-04-01

    The comet model presented in the paper is based on the radiation transfer code ratran (M. R. Hogerheijde & F. F. S. van der Tak, ApJ, 615, 531 [2004]), an accelerated Monte Carlo code to calculate the line excitation and the emerging line intensities. For a given density profile, ratran determines the level population and, in a second step, calculates the spectral line emission for a given telescope beam, source distance, and velocity resolution. The numerical simulations for water-line emission in cometary coma show that the line excitation varies considerably on radii smaller than the projected telescope beam at the source distance for the models and telescope parameter considered in the paper. Thus, in our calculation, the water excitation in the second step was incorrectly sampled and the emission predictions were inexact, particularly for weak, optically thin lines.4 This problem also resulted from a limitation of the ratran version with respect to the spatial sampling of the intensity distribution for a convolution with a telescope beam. The relevant part of the ratran code was corrected by the authors with the May 2003 release of their software (F. F. S. van der Tak 2006, private communication). To correct this issue, we redid the numerical models presented in the original paper using the May 2003 release of the ratran software and made tests to ensure that the spatial intensity distribution used to calculate the emission detected by a given telescope beam is sampled with a sufficient spatial resolution. The corrected version of the radiation transfer model does not affect the first step, where the level population is calculated; however, the beam-averaged line intensities calculated from the level populations are generally lower than quoted in the original paper. The impact is moderate for the ground-state transitions and for the models with a water production rate of Q29=1 (M1, M6, M7), but can be substantial for the emission in weak comets and the H218O

  12. Power supplies for IRFEL beam transport line magnets

    International Nuclear Information System (INIS)

    The Infrared Free Electron Laser (IRFEL) is being developed at RRCAT. Current regulated, dc power supplies are required for various electromagnets in its beam transport line. These are rated for 13 A and 15 V (maximum) with output current stability of ±100 ppm. In all, 60 power supplies have been developed, including the spare ones. In order to facilitate timely development of these power supplies, uniform power converter scheme for all power supplies has been followed and all associated low- power electronics, e.g. sensing, fault handling, auxiliary power sources, handling remote interface have been standardized. The topology used in these power supplies is two-switch forward converter operating with variable frequency PWM (VFPWM) control in the range from 20 kHz to 100 kHz. After the design, simulation studies, prototype development and rigorous testing of the prototype at RRCAT, the bulk production, testing and evaluation was carried out with the involvement of industry. This paper describes power supply design principles, highlights its salient features, shares the development experience and discusses the test results. (author)

  13. The Broad-Lined Type Ic SN 2012ap and the Nature of Relativistic Supernovae Lacking a Gamma-Ray Burst Detection

    Science.gov (United States)

    Milisavljevic, D.; Margutti, R.; Parrent, J. T.; Soderberg, A. M.; Fesen, R. A.; Mazzali, P.; Maeda, K.; Sanders, N. E.; Cenko, S. B.; Silverman, J. M.

    2014-01-01

    We present ultraviolet, optical, and near-infrared observations of SN2012ap, a broad-lined Type Ic supernova in the galaxy NGC 1729 that produced a relativistic and rapidly decelerating outflow without a gamma-ray burst signature. Photometry and spectroscopy follow the flux evolution from -13 to +272 days past the B-band maximum of -17.4 +/- 0.5 mag. The spectra are dominated by Fe II, O I, and Ca II absorption lines at ejecta velocities of v approx. 20,000 km s(exp. -1) that change slowly over time. Other spectral absorption lines are consistent with contributions from photospheric He I, and hydrogen may also be present at higher velocities (v approx. greater than 27,000 km s(exp. -1)). We use these observations to estimate explosion properties and derive a total ejecta mass of 2.7 Solar mass, a kinetic energy of 1.0×1052 erg, and a (56)Ni mass of 0.1-0.2 Solar mass. Nebular spectra (t > 200 d) exhibit an asymmetric double-peaked [O I] lambda lambda 6300, 6364 emission profile that we associate with absorption in the supernova interior, although toroidal ejecta geometry is an alternative explanation. SN2012ap joins SN2009bb as another exceptional supernova that shows evidence for a central engine (e.g., black-hole accretion or magnetar) capable of launching a non-negligible portion of ejecta to relativistic velocities without a coincident gamma-ray burst detection. Defining attributes of their progenitor systems may be related to notable properties including above-average environmental metallicities of Z approx. greater than Solar Z, moderate to high levels of host-galaxy extinction (E(B -V ) > 0.4 mag), detection of high-velocity helium at early epochs, and a high relative flux ratio of [Ca II]/[O I] > 1 at nebular epochs. These events support the notion that jet activity at various energy scales may be present in a wide range of supernovae.

  14. THE BROAD-LINED Type Ic SN 2012ap AND THE NATURE OF RELATIVISTIC SUPERNOVAE LACKING A GAMMA-RAY BURST DETECTION

    Energy Technology Data Exchange (ETDEWEB)

    Milisavljevic, D.; Margutti, R.; Parrent, J. T.; Soderberg, A. M.; Sanders, N. E.; Kamble, A.; Chakraborti, S.; Drout, M. R.; Kirshner, R. P. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Fesen, R. A. [Department of Physics and Astronomy, Dartmouth College, 6127 Wilder Laboratory, Hanover, NH 03755 (United States); Mazzali, P. [Astrophysics Research Institute, Liverpool John Moores University, Liverpool L3 5RF (United Kingdom); Maeda, K. [Department of Astronomy, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-8502 (Japan); Cenko, S. B. [Astrophysics Science Division, NASA Goddard Space Flight Center, Mail Code 661, Greenbelt, MD 20771 (United States); Silverman, J. M. [University of Texas at Austin, 1 University Station C1400, Austin, TX 78712-0259 (United States); Filippenko, A. V. [Department of Astronomy, University of California, Berkeley, CA 94720-3411 (United States); Pickering, T. E. [Southern African Large Telescope, P.O. Box 9, Observatory 7935, Cape Town (South Africa); Kawabata, K. [Hiroshima Astrophysical Science Center, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526 (Japan); Hattori, T. [Subaru Telescope, National Astronomical Observatory of Japan, Hilo, HI 96720 (United States); Hsiao, E. Y. [Carnegie Observatories, Las Campanas Observatory, Colina El Pino, Casilla 601 (Chile); Stritzinger, M. D., E-mail: dmilisav@cfa.harvard.edu [Department of Physics and Astronomy, Aarhus University, Ny Munkegade, DK-8000 Aarhus C (Denmark); and others

    2015-01-20

    We present ultraviolet, optical, and near-infrared observations of SN 2012ap, a broad-lined Type Ic supernova in the galaxy NGC 1729 that produced a relativistic and rapidly decelerating outflow without a gamma-ray burst signature. Photometry and spectroscopy follow the flux evolution from –13 to +272 days past the B-band maximum of –17.4 ± 0.5 mag. The spectra are dominated by Fe II, O I, and Ca II absorption lines at ejecta velocities of v ≈ 20,000 km s{sup –1} that change slowly over time. Other spectral absorption lines are consistent with contributions from photospheric He I, and hydrogen may also be present at higher velocities (v ≳ 27,000 km s{sup –1}). We use these observations to estimate explosion properties and derive a total ejecta mass of ∼2.7 M {sub ☉}, a kinetic energy of ∼1.0 × 10{sup 52} erg, and a {sup 56}Ni mass of 0.1-0.2 M {sub ☉}. Nebular spectra (t > 200 days) exhibit an asymmetric double-peaked [O I] λλ6300, 6364 emission profile that we associate with absorption in the supernova interior, although toroidal ejecta geometry is an alternative explanation. SN 2012ap joins SN 2009bb as another exceptional supernova that shows evidence for a central engine (e.g., black hole accretion or magnetar) capable of launching a non-negligible portion of ejecta to relativistic velocities without a coincident gamma-ray burst detection. Defining attributes of their progenitor systems may be related to notable observed properties including environmental metallicities of Z ≳ Z {sub ☉}, moderate to high levels of host galaxy extinction (E(B – V) > 0.4 mag), detection of high-velocity helium at early epochs, and a high relative flux ratio of [Ca II]/[O I] >1 at nebular epochs. These events support the notion that jet activity at various energy scales may be present in a wide range of supernovae.

  15. Effects of Escherichia coli Challenge and Transport Stress on Hematology and Serum Chemistry Values of Three Genetic Lines of Turkeys

    Science.gov (United States)

    Males and females from 3 genetic lines of turkeys were compared for their response to an Escherichia coli airsac challenge followed by transport stress (Transport). The turkey lines were a slow growing line selected for increased egg production (Egg line), a fast growing line selected for increased...

  16. Error analysis in post linac to driver linac transport beam line of RAON

    Science.gov (United States)

    Kim, Chanmi; Kim, Eun-San

    2016-07-01

    We investigated the effects of magnet errors in the beam transport line connecting the post linac to the driver linac (P2DT) in the Rare Isotope Accelerator in Korea (RAON). The P2DT beam line is bent by 180-degree to send the radioactive Isotope Separation On-line (ISOL) beams accelerated in Linac-3 to Linac-2. This beam line transports beams with multi-charge state 132Sn45,46,47. The P2DT beam line includes 42 quadrupole, 4 dipole and 10 sextupole magnets. We evaluate the effects of errors on the trajectory of the beam by using the TRACK code, which includes the translational and the rotational errors of the quadrupole, dipole and sextupole magnets in the beam line. The purpose of this error analysis is to reduce the rate of beam loss in the P2DT beam line. The distorted beam trajectories can be corrected by using six correctors and seven monitors.

  17. Control system of HLS transport line and Linac focusing power supplies

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The control system of transport line and Linac focusing power supplies of Hefei Light Source was built upon Experimental Physics and Industrial Control System. The hardware construction, software design and performance test of the control system are described.

  18. An automated system for measuring the mass flowrate of powders in transport lines

    Science.gov (United States)

    Littman, Howard; Morgan, Morris B., III; Prapas, Demetrios K.; Rubel, Glen O.

    1990-08-01

    A new automated particle transport (APT) system has been developed for studying the dissemination of bulk powders into deagglomerated aerosols. It consists of a 1.12-inch ID transport line with a spout-fluidized bed feeder. The particles are transported from an aerated annulus into the transport line and collected in a closed can or bag filter. Two separate feed lines supply the air necessary to operate the transport line and aerate the particles in order that they flow smoothly into the transport line. An IBM PC AT computer clone equipped with a data translation DT 2806 multifunction input-output board and A to D and D to A modules (DTX 311 and 328) is used for both control and data acquisition. A fluid mechanical model of the flow has been developed and the APT system will be used to verify it. Experiments will be conducted to measure the choking velocity, drag coefficient, fluid and particle flowrates, and pressure distribution in the line.

  19. Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line

    OpenAIRE

    Cruz, M. Teresa; Gonçalo, Margarida; Figueiredo, Américo; Carvalho, Arsélio P.; Duarte, Carlos B.; Lopes, M. Celeste

    2004-01-01

    Nuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO4) and increases the ...

  20. Quasilinear Line Broadened Model for Energetic Particle Transport

    Science.gov (United States)

    Ghantous, Katy; Gorelenkov, Nikolai; Berk, Herbert

    2011-10-01

    We present a self-consistent quasi-linear model that describes wave-particle interaction in toroidal geometry and computes fast ion transport during TAE mode evolution. The model bridges the gap between single mode resonances, where it predicts the analytically expected saturation levels, and the case of multiple modes overlapping, where particles diffuse across phase space. Results are presented in the large aspect ratio limit where analytic expressions are used for Fourier harmonics of the power exchange between waves and particles, . Implemention of a more realistic mode structure calculated by NOVAK code are also presented. This work is funded by DOE contract DE-AC02-09CH11466.

  1. CDK2-AP1基因过表达对乳腺癌MCF-7细胞增殖及周期的影响%Effect of CDK2-AP1 gene over-expression on proliferation and cell cycle regulation of breast cancer cell line MCF-7

    Institute of Scientific and Technical Information of China (English)

    关晓燕; 周卫兵; 黄隽; 王龙云; 廖遇平

    2012-01-01

    Objective: To over-express cyclin-dependent kinase 2-associated protein 1 (CDK2-AP1) gene, and investigate its effect on the proliferation and cell cycle regulation in breast cancer cell line MCF-7. Methods: CDK2-AP1 gene coding region was cloned into lentivirus vector. Lentivirus particles were infected into MCF-7 cells to upregulate the expression of CDK2-AP1 gene. The expression level of CDK2-AP1 was detected at both mRNA and protein levels by real-time PCR and Western blot. MTT assay, colony formatting assay, and flow cytometry were performed to detect the change of proliferation and cell cycle in MCF-7 cells. We examined the expression of cell cycle associated genes (CDK2, CDK4, P16Ink4A, and P2lCiP1/Wafl) followed by CDK2-AP1 over-expression by Western blot.Results: CDK2-AP1 gene was up-regulated significantly at both mRNA (6.94 folds) and protein level. MTT based growth curve, colony formatting assay and flow cytometry showed that CDK2- API over-expression lentivirus inhibited the proliferation of MCF-7 cells with statistical difference (P<0.05). In addition, with CDK2-AP1 over-expression, MCF-7 cells were arrested in G1 phase accompanied by apoptosis. Western blot showed that the expression level of P21Clpl/wafl and P16Int4A was upregulated, while the expression level of CDK2 and CDK4, members of the CDK family, was downregulated.Conclusion: CDK2-AP1 gene plays a cancer suppressor role in breast cancer. Its function includes inhibiting the proliferation of MCF-7 cells and arresting the cell cycle in G, phase.%目的:通过过表达手段上调细胞周期调节蛋白依赖性激酶2-关联蛋白1(CDK2-AP1)基因在乳腺癌细胞MCF-7中的表达,并观察其对MCF-7细胞生长和细胞周期调控的作用.方法:将CDK2-AP1基因的编码框构建于慢病毒表达载体,导入MCF-7细胞,应用实时定量PCR和Western印迹验证CDK2-AP1基因mRNA和蛋白的表达效率.利用MTT法绘制生长曲线、克隆形成实验观察CDK2-AP1

  2. Spin- and valley-polarized transport across line defects in monolayer MoS2

    Science.gov (United States)

    Pulkin, Artem; Yazyev, Oleg V.

    2016-01-01

    We address the ballistic transmission of charge carriers across ordered line defects in monolayer transition metal dichalcogenides. Our study reveals the presence of a transport gap driven by spin-orbit interactions, spin and valley filtering, both stemming from a simple picture of spin and momentum conservation, as well as the electron-hole asymmetry of charge-carrier transmission. Electronic transport properties of experimentally observed ordered line defects in monolayer MoS2, in particular, the vacancy lines and inversion domain boundaries, are further investigated using first-principles Green's function methodology. Our calculations demonstrate the possibility of achieving nearly complete spin polarization of charge carriers in nanoelectronic devices based on engineered periodic line defects in monolayer transition metal dichalcogenides, thus suggesting a practical scheme for all-electric control of spin transport.

  3. APS Science 2007.

    Energy Technology Data Exchange (ETDEWEB)

    2008-05-30

    This report provides research highlights from the Advanced Photon Source (APS). Although these highlights represent less than 10% of the published work from the APS in 2007, they give a flavor of the diversity and impact of user research at the facility. In the strategic planning the aim is to foster the growth of existing user communities and foresee new areas of research. This coming year finds the APS engaged in putting together, along with the users, a blueprint for the next five years, and making the case for a set of prioritized investments in beamlines, the accelerator, and infrastructure, each of which will be transformational in terms of scientific impact. As this is written plans are being formulated for an important user workshop on October 20-21, 2008, to prioritize strategic plans. The fruit from past investments can be seen in this report. Examples include the creation of a dedicated beamline for x-ray photon correlation spectroscopy at Sector 8, the evolution of dedicated high-energy x-ray scattering beamlines at sectors 1 and 11, a dedicated imaging beamline at Sector 32, and new beamlines for inelastic scattering and powder diffraction. A single-pulse facility has been built in collaboration with Sector 14 (BioCARS) and Phil Anfinrud at the National Institutes of Health, which will offer exceptionally high flux for single-pulse diffraction. The nanoprobe at Sector 26, built and operated jointly by the Argonne Center for Nanoscale Materials and the X-ray Operations and Research (XOR) section of the APS X-ray Science Division, has come on line to define the state of the art in nanoscience.

  4. Spin helical states and spin transport of the line defect in silicene lattice

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Mou; Chen, Dong-Hai; Wang, Rui-Qiang [Laboratory of Quantum Engineering and Quantum Materials, School of Physics and Telecommunication Engineering, South China Normal University, Guangzhou 510006 (China); Bai, Yan-Kui, E-mail: ykbai@semi.ac.cn [College of Physical Science and Information Engineering and Hebei Advance Thin Films Laboratory, Hebei Normal University, Shijiazhuang, Hebei 050024 (China)

    2015-02-06

    We investigated the electronic structure of a silicene-like lattice with a line defect under the consideration of spin–orbit coupling. In the bulk energy gap, there are defect related bands corresponding to spin helical states localized beside the defect line: spin-up electrons flow forward on one side near the line defect and move backward on the other side, and vice versa for spin-down electrons. When the system is subjected to random distribution of spin-flipping scatterers, electrons suffer much less spin-flipped scattering when they transport along the line defect than in the bulk. An electric gate above the line defect can tune the spin-flipped transmission, which makes the line defect as a spin-controllable waveguide. - Highlights: • Band structure of silicene with a line defect. • Spin helical states around the line defect and their probability distribution features. • Spin transport along the line defect and that in the bulk silicene.

  5. Design of the beam transport line and injection system of the compact storage ring for TTX

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    In this article, we present the design of the beam transport line and injection system of the compact storage ring for the Tsinghua Thomson scattering X-ray (TTX) source. The layout of the beam transport line fits in with the limited available space. The injection system is simplified, consisting of only one single kicker; the stray field on the reference orbit is also reduced without the septum magnet. We choose a travelling wave kicker and present both 2D and 3D simulations for the structure design.

  6. Enhancing the accelerated beam current in the booster synchrotron by optimizing the transport line beam propagation

    Indian Academy of Sciences (India)

    Saini R S; Tyagi Y; Ghodke A D; Puntambekar T A

    2016-04-01

    In this paper, we present the results of transverse beam emittance and twiss parameter measurement of an electron beam, delivered by a 20 MeV microtron which is used as a pre-injector system for a booster synchrotron in the Indus Accelerator Facility at RRCAT Indore. Based on these measured beam parameters, beam optics of a transport line was optimized and its results are alsodiscussed in this paper. This beam transport line is used to transport the electron beam from the 20MeV microtron to the booster synchrotron. The booster synchrotron works as a main injector for Indus-1 and Indus-2 synchrotron radiation facilities. To optimize the beam optics of a transport linefor proper beam transmission through the line as well as to match the beam twiss parameters at the beam injection point of another accelerator, it is necessary to know the transverse beam emittance and twiss parameters of the beam coming from the first one. A MATLAB-based GUI program has been developed to calculate the beam emittance and twiss parameters, using quadrupole scanmethod. The measured parameters have been used for beam transport line optimization and twiss parameters matching at booster injection point. After this optimization, an enhancement of ∼50% beam current has been observed in the booster synchrotron.

  7. High-Accuracy Spectral Lines for Radiation Transport in Stellar Atmospheres

    OpenAIRE

    Amit R. Sharma; Braams, Bastiaan J.; Bowman, Joel M.; Robert Warmbier; Ralf Schneider; Hauschildt, Peter H.

    2008-01-01

    The theory of radiative transfer is an important element for the understanding of the spectral signature and physical structure of stellar atmosphere. PHOENIX1 is a such, very general non-Local Thermodynamic Equilibrium(NLTE) stellar atmosphere computer code which can handle very large model atoms/molecules as well as line blanketing by hundreds of millions of atomic and molecular lines. The code is used to compute model atmospheres and synthetic spectra (solution of the radiative transport e...

  8. Cells in G2/M phase increased in human nasopharyngeal carcinoma cell line by EBV-LMP1 through activation of NF-κB and AP-1

    Institute of Scientific and Technical Information of China (English)

    LIN DENG; JING YANG; XIAO RONG ZHAO; XI YUN DENG; LIANG ZENG; HUAN HUA GU; MIN TANG; YA CAO

    2003-01-01

    Although previous studies showed that the principal oncoprotein encoded by Epstein-Barr virus, latentmembrane protein 1(LMP1), could induce the nasopharyngeal carcinoma cells in G2/M phase increased, littleis known about the target molecules and mechanisms. The present study demonstrated that LMP1 couldinduce the accumulation of p53 protein and upregulate its transactivity in a dose dependent manner, whichresulted in the decrease of the kinase activity of cdc2/cyclin B complex and inducing arrest at G2/M phasethrough the activation of NF-κB and AP-1 signaling pathways, and the effect of NF-κB was more obviousthan that of AP-1. This study provided some significant evidence for further elucidating the molecularmechanisms that LMP1 had effects on the surveillance mechanism of cell cycle and promoting the survivalof transformed cells and tumorigenesis.

  9. Postendocytic sorting of constitutively internalized dopamine transporter in cell lines and dopaminergic neurons

    DEFF Research Database (Denmark)

    Eriksen, Jacob; Bjørn-Yoshimoto, Walden Emil; Jørgensen, Trine Nygaard;

    2010-01-01

    lines, we fused the one-transmembrane segment protein Tac to DAT, thereby generating a transporter (TacDAT) with an extracellular antibody epitope suited for trafficking studies. TacDAT was functional and endocytosed constitutively in HEK293 cells. According to an ELISA-based assay, TacDAT intracellular......The dopamine transporter (DAT) mediates reuptake of released dopamine and is the target for psychostimulants, such as cocaine and amphetamine. DAT undergoes marked constitutive endocytosis, but little is known about the fate and sorting of the endocytosed transporter. To study DAT sorting in cells...

  10. 8 CFR 241.9 - Notice to transportation line of alien's removal.

    Science.gov (United States)

    2010-01-01

    ... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Notice to transportation line of alien's removal. 241.9 Section 241.9 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS APPREHENSION AND DETENTION OF ALIENS ORDERED REMOVED Post-hearing Detention and Removal §...

  11. Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line

    OpenAIRE

    Cruz, MT; Gonçalo, Margarida; A. Figueiredo; Carvalho, AP; Duarte, CB

    2004-01-01

    Nuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO(4)) and increases the expression of the i...

  12. Transport and dosimetric solutions for the ELIMED laser-driven beam line

    Energy Technology Data Exchange (ETDEWEB)

    Cirrone, G.A.P. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Romano, F. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Medical Physics School, University of Catania, Via S. Sofia 64 - 95125 Catania (Italy); Scuderi, V. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Institute of Physics ASCR, v.v.i. (FZU), ELI-Beamlines Project, Na Slovance 2, 182 21 Prague (Czech Republic); Amato, A. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Candiano, G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Medical Physics School, University of Catania, Via S. Sofia 64 - 95125 Catania (Italy); Cuttone, G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Giove, D. [INFN Sezione di Milano, Via Celoria 16, Milano (Italy); Korn, G.; Krasa, J. [Institute of Physics ASCR, v.v.i. (FZU), ELI-Beamlines Project, Na Slovance 2, 182 21 Prague (Czech Republic); Leanza, R. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Universitá degli Studi di Catania, Dipartimento di Fisica e Astronomia, Via S. Sofia 64, Catania (Italy); Manna, R. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Maggiore, M. [INFN-LNL, Viale dell' Universitá 2 - 35020 Legnaro (PD) (Italy); Marchese, V. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Margarone, D. [Institute of Physics ASCR, v.v.i. (FZU), ELI-Beamlines Project, Na Slovance 2, 182 21 Prague (Czech Republic); Milluzzo, G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Universitá degli Studi di Catania, Dipartimento di Fisica e Astronomia, Via S. Sofia 64, Catania (Italy); Petringa, G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Sabini, M.G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Azienda Ospedaliera Cannizzaro, Via Messina 829 - 95100 Catania (Italy); Schillaci, F. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Medical Physics School, University of Catania, Via S. Sofia 64 - 95125 Catania (Italy); and others

    2015-10-01

    Within 2017, the ELIMED (ELI-Beamlines MEDical applications) transport beam-line and dosimetric systems for laser-generated beams will be installed at the ELI-Beamlines facility in Prague (CZ), inside the ELIMAIA (ELI Multidisciplinary Applications of laser–Ion Acceleration) interaction room. The beam-line will be composed of two sections: one in vacuum, devoted to the collecting, focusing and energy selection of the primary beam and the second in air, where the ELIMED beam-line dosimetric devices will be located. This paper briefly describes the transport solutions that will be adopted together with the main dosimetric approaches. In particular, the description of an innovative Faraday Cup detector with its preliminary experimental tests will be reported.

  13. Investigation of oxygen impurity transport using the O4+ visible spectral line in the Aditya tokamak

    International Nuclear Information System (INIS)

    Intense visible lines from Be-like oxygen impurity are routinely observed in the Aditya tokamak. The spatial profile of brightness of a Be-like oxygen spectral line (2p3p 3D3–2p3d 3F4) at 650.024 nm is used to investigate oxygen impurity transport in typical discharges of the Aditya tokamak. A 1.0 m multi-track spectrometer (Czerny–Turner) capable of simultaneous measurements from eight lines of sight is used to obtain the radial profile of brightness of O4+ spectral emission. The emissivity profile of O4+ spectral emission is obtained from the spatial profile of brightness using an Abel-like matrix inversion. The oxygen transport coefficients are determined by reproducing the experimentally measured emissivity profiles of O4+, using a one-dimensional empirical impurity transport code, STRAHL. Much higher values of the diffusion coefficient compared with the neo-classical values are observed in both the high magnetic field edge region (Dinboardmax∼30 m2 s-1) and the low magnetic field edge region (Doutboardmax∼45 m2 s-1) of typical Aditya ohmic plasmas, which seems to be due to fluctuation-induced transport. The diffusion coefficient at the limiter radius in the low-field (outboard) region is typically ∼ twice as high as that at the limiter radius in the high-field (inboard) region. (paper)

  14. Railroad Lines - RAILROAD_100K_NTAD_IN: Railroad Network in Indiana (National Transportation Atlas Database, 1:100,000, Line Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — RAILROAD_NTAD_100K_SW is a 1:100,000 scale line shapefile which is a subset of the rail network. Bureau of Transportation Statistics metadata states - "The Rail...

  15. Ambipolar transport via trapped-electron whistler instability along open magnetic field lines.

    Science.gov (United States)

    Guo, Zehua; Tang, Xian-Zhu

    2012-09-28

    An open field line plasma is bounded by a chamber wall which intercepts the magnetic field. Steady state requires an upstream plasma source balancing the particle loss to the boundary. In cases where the electrons have a long mean free path, ambipolarity in parallel transport critically depends on collisionless detrapping of the electrons via wave-particle interaction. The trapped-electron whistler instability, whose nonlinear saturation produces a spectrum of whistler waves that is responsible for the electron detrapping flux, is shown to be an unusually robust kinetic instability, which is essential to the universality of the ambipolar constraint in plasma transport.

  16. AP Music Theory Applied

    Science.gov (United States)

    Spieker, Matthew H.

    2016-01-01

    Some American high schools include Advanced Placement (AP) Music Theory within their course offerings. Students who pass the AP exam can receive college credit either as a music or humanities credit. An AP class, however, offers music students more than future college credit; it ultimately improves musicianship skills and promotes deeper…

  17. Experimental determination of beam loss point in transport line-2 of Indus Accelerator Complex

    International Nuclear Information System (INIS)

    Radiation field in the Indus-1 SRS Experimental hall during 550 MeV electron beam injection into Transport Line-3 (TL-3)/Indus-2 was found to be higher than during 450 MeV beam injection to Transport line -2 (TL-2)/Indus-1. Experimental investigation was carried out to find out the location of beam loss. For the investigation, Ion chamber based detectors viz direct reading dosimeters (passive detectors) and beam loss monitors (active) were used. The beam loss point was observed near Sputter Ion Pump-5 (SIP-5) of TL-2, in Indus-1 area. The result was confirmed by induced activity profile measurements of the transport lines (TL-2/TL-3) during shut down. In order to reduce the radiation level in Indus-1 hall, two tenth value layers of lead shielding was put near TL2. Later on, correction in the beam optics by beam dynamics section reduced the beam losses at SIP-5 location, thereby reducing the radiation fields in Indus-1 hall substantially. The paper describes the measurement and the results in detail. (author)

  18. The design of the public transport lines with the use of the fast genetic algorithm

    Directory of Open Access Journals (Sweden)

    Aleksander Król

    2015-09-01

    Full Text Available Background: The growing role of public transport and the pressure of economic criteria requires the new optimization tools for process of public transport planning. These problems are computationally very complex, thus it is preferable to use various approximate methods, leading to a good solution within an acceptable time. Methods: One of such method is the genetic algorithm mimicking the processes of evolution and natural selection in the nature. In this paper, the different variants of the public transport lines layout are subjected to the artificial selection. The essence of the proposed approach is a simplified method of calculating the value of the fit function for a single individual, which brings relatively short computation time even for large jobs. Results: It was shown that despite the introduced simplifications the quality of the results is not worsened. Using the data obtained from KZK GOP (Communications Municipal Association of Upper Silesian Industrial Region the described algorithm was used to optimize the layout of the network of bus lines located within the borders of Katowice. Conclusion: The proposed algorithm was applied to a real, very complex network of public transportation and a possibility of a significant improvement of its efficiency was indicated. The obtained results give hope that the presented model, after some improvements can be the basis of the scientific method, and in a consequence of a further development to find practical application.

  19. Transport line for a multi-staged laser-plasma acceleration: DACTOMUS

    Energy Technology Data Exchange (ETDEWEB)

    Chancé, Antoine, E-mail: antoine.chance@cea.fr [CEA, IRFU, SACM, Centre de Saclay, F-91191 Gif-sur-Yvette (France); Delferrière, Olivier; Schwindling, Jérôme [CEA, IRFU, SACM, Centre de Saclay, F-91191 Gif-sur-Yvette (France); Bruni, Christelle; Delerue, Nicolas [LAL, UMR9607, CNRS and Université Paris Sud, Orsay (France); Specka, Arnd [LLR, UMR7638, CNRS and Ecole Polytechnique, Palaiseau (France); Cros, Brgitte; Maynard, Gillies; Paradkar, Bhooshan S. [LPGP, UMR8578, CNRS and Université Paris Sud, Orsay (France); Mora, Patrick [CPhT, UMR7644, CNRS and Ecole Polytechnique, Palaiseau (France)

    2014-03-11

    Laser-plasma acceleration is one of the most promising techniques to reach very high acceleration gradients up to a few hundreds of GeV/m. In order to push this acceleration scheme in the domain of the very high energies, the CILEX project was launched with the laser APOLLON. One of the main topics of this project is to study multi-staged acceleration. It consists in generating and pre-accelerating electrons in a first laser-plasma stage, to transport them up to a second stage where the electrons are accelerated again thanks to another laser pulse. The DACTOMUS project, based on a collaboration CEA-IRFU, CEA-IRAMIS, LAL, LPGP, LULI and LLR, aims at the study and realization of such a transfer line between these two stages. Firstly, a prototype will be developed and tested by the groups of CEA-IRAMIS-SPAM, LPGP, and LULI on the UHI100 facility (CEA-SPAM). This collaboration must enable to realize the first acceleration stage. For the transport line prototype, the main difficulties are to realize a very compact and energy accepting line with diagnostics to characterize the electron beam. We will present here the optics of this line, its performances and the inserted diagnostics.

  20. Transport line for a multi-staged laser-plasma acceleration: DACTOMUS

    Science.gov (United States)

    Chancé, Antoine; Delferrière, Olivier; Schwindling, Jérôme; Bruni, Christelle; Delerue, Nicolas; Specka, Arnd; Cros, Brgitte; Maynard, Gillies; Paradkar, Bhooshan S.; Mora, Patrick

    2014-03-01

    Laser-plasma acceleration is one of the most promising techniques to reach very high acceleration gradients up to a few hundreds of GeV/m. In order to push this acceleration scheme in the domain of the very high energies, the CILEX project was launched with the laser APOLLON. One of the main topics of this project is to study multi-staged acceleration. It consists in generating and pre-accelerating electrons in a first laser-plasma stage, to transport them up to a second stage where the electrons are accelerated again thanks to another laser pulse. The DACTOMUS project, based on a collaboration CEA-IRFU, CEA-IRAMIS, LAL, LPGP, LULI and LLR, aims at the study and realization of such a transfer line between these two stages. Firstly, a prototype will be developed and tested by the groups of CEA-IRAMIS-SPAM, LPGP, and LULI on the UHI100 facility (CEA-SPAM). This collaboration must enable to realize the first acceleration stage. For the transport line prototype, the main difficulties are to realize a very compact and energy accepting line with diagnostics to characterize the electron beam. We will present here the optics of this line, its performances and the inserted diagnostics.

  1. A Central Role for JNK/AP-1 Pathway in the Pro-Oxidant Effect of Pyrrolidine Dithiocarbamate through Superoxide Dismutase 1 Gene Repression and Reactive Oxygen Species Generation in Hematopoietic Human Cancer Cell Line U937

    Science.gov (United States)

    Collin, Pascal; Lomri, Abderrahim

    2015-01-01

    Pyrrolidine dithiocarbamate (PDTC) known as antioxidant and specific inhibitor of NF-κB was also described as pro-oxidant by inducing cell death and reactive oxygen species (ROS) accumulation in cancer. However, the mechanism by which PDTC indices its pro-oxidant effect is unknown. Therefore, we aimed to evaluate the effect of PDTC on the human Cu/Zn superoxide dismutase 1 (SOD1) gene transcription in hematopoietic human cancer cell line U937. We herein show for the first time that PDTC decreases SOD1 transcripts, protein and promoter activity. Furthermore, SOD1 repression by PDTC was associated with an increase in oxidative stress as evidenced by ROS production. Electrophoretic mobility-shift assays (EMSA) show that PDTC increased binding of activating protein-1 (AP-1) in dose dependent-manner suggesting that the MAPkinase up-stream of AP-1 is involved. Ectopic NF-κB p65 subunit overexpression had no effect on SOD1 transcription. In contrast, in the presence of JNK inhibitor (SP600125), p65 induced a marked increase of SOD1 promoter, suggesting that JNK pathway is up-stream of NF-κB signaling and controls negatively its activity. Indeed, using JNK deficient cells, PDTC effect was not observed nether on SOD1 transcription or enzymatic activity, nor on ROS production. Finally, PDTC represses SOD1 in U937 cells through JNK/c-Jun phosphorylation. Taken together, these results suggest that PDTC acts as pro-oxidant compound in JNK/AP-1 dependent-manner by repressing the superoxide dismutase 1 gene leading to intracellular ROS accumulation. PMID:25996379

  2. FUZZY COMPREHENSIVE EVALUATION OF CONTAINER TRANSPORTATION MODES ALONG THE CHANGJIANG RIVER MAIN LINE AND ITS DELTA AREA

    Institute of Scientific and Technical Information of China (English)

    ZHANG Shi-yu; XIAO Han-liang

    2003-01-01

    The Changjiang River (Yangtze) is one of the fastest growth areas of container transportation in Chi-na. Rail, road and water transportation have competed against each other for container transportation in the Chang-jiang River main line and its delta area. It is of significance to assess these different transportation modes scientifi-cally in order to organize container transportation efficiently in this area and make decision for integral plan and construction of transportation system in this area. This paper outlines application of fuzzy comprehensive evaluation to appraise different modes of typical direction of containers. Twelve assessment indexes were decided. Membership functions were formulated. Evaluation results indicated that road transportation was optimal mode in the Changjiang River delta area, however water transportation was the primary way in the Changjiang River main line.

  3. The AP-1A and AP-1B clathrin adaptor complexes define biochemically and functionally distinct membrane domains

    OpenAIRE

    Fölsch, Heike; Pypaert, Marc; Maday, Sandra; Pelletier, Laurence; Mellman, Ira

    2003-01-01

    Most epithelial cells contain two AP-1 clathrin adaptor complexes. AP-1A is ubiquitously expressed and involved in transport between the TGN and endosomes. AP-1B is expressed only in epithelia and mediates the polarized targeting of membrane proteins to the basolateral surface. Both AP-1 complexes are heterotetramers and differ only in their 50-kD μ1A or μ1B subunits. Here, we show that AP-1A and AP-1B, together with their respective cargoes, define physically and functionally distinct membra...

  4. Initial diagnostics commissioning results for the APS injector subsystems

    Science.gov (United States)

    Lumpkin, A.; Chung, Y.; Kahana, E.; Patterson, D.; Sellyey, W.; Smith, T.; Wang, X.

    1995-05-01

    In recent months the first beams have been introduced into the various injector subsystems of the Advanced Photon Source (APS). An overview will be given of the diagnostics results on beam profiling, beam position monitors (BPMs), loss rate monitors (LRMs), current monitors (CMs), and photon monitors on the low energy transport lines, positron accumulator ring (PAR), and injector synchrotron (IS). Initial measurements have been done with electron beams at energies from 250 to 450 MeV and 50 to 400 pC per macrobunch. Operations in single turn and stored beam conditions were diagnosed in the PAR and IS.

  5. Poly(acrylic acid) coating induced 2-line ferrihydrite nanoparticle transport in saturated porous media

    Energy Technology Data Exchange (ETDEWEB)

    Xiang, Aishuang [Princeton University, Chemical and Biological Engineering Department (United States); Yan, Weile [Texas Tech University, Civil and Environmental Engineering (United States); Koel, Bruce E., E-mail: bkoel@princeton.edu [Princeton University, Chemical and Biological Engineering Department (United States); Jaffe, Peter R., E-mail: jaffe@princeton.edu [Princeton University, Civil and Environmental Engineering Department (United States)

    2013-07-15

    Iron oxide and iron nanoparticles (NPs) have been used effectively for environmental remediation, but are limited in their applications by strong retention in groundwater-saturated porous media. For example, delivery of NPs to large groundwater reservoirs would require large numbers of injection wells. To address this problem, we have explored polymer coatings as a surface engineering strategy to enhance transport of oxide nanoparticles in porous media. We report here on our studies of 2-line ferrihydrite NPs and the influence of poly (acrylic acid) (PAA) polymer coatings on the colloidal stability and transport in natural sand-packed column tests simulating flow in groundwater-saturated porous media. Measurements were also made of zeta potential, hydrodynamic diameter, and polymer adsorption and desorption properties. The coated NPs have a diameter range of 30-500 nm. We found that NP transport was improved by PAA coating and that the transport properties could be tuned by adjusting the polymer concentration. Our results demonstrate that a high stability of oxide particles and improved transport can be achieved in groundwater-saturated porous media by introducing negatively charged polyelectrolytes and optimizing polymer concentrations.

  6. Diffusive Heat Transport in Budyko's Energy Balance Climate Model with a Dynamic Ice Line

    CERN Document Server

    Walsh, James

    2016-01-01

    M. Budyko and W. Sellers independently introduced seminal energy balance climate models in 1969, each with a goal of investigating the role played by positive ice albedo feedback in climate dynamics. In this paper we replace the relaxation to the mean horizontal heat transport mechanism used in the models of Budyko and Sellers with diffusive heat transport. We couple the resulting surface temperature equation with an equation for movement of the edge of the ice sheet (called the ice line), recently introduced by E. Widiasih. We apply the spectral method to the temperature-ice line system and consider finite approximations. We prove there exists a stable equilibrium solution with a small ice cap, and an unstable equilibrium solution with a large ice cap, for a range of parameter values. If the diffusive transport is too efficient, however, the small ice cap disappears and an ice free Earth becomes a limiting state. In addition, we analyze a variant of the coupled diffusion equations appropriate as a model for ...

  7. Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant.

    Science.gov (United States)

    Fang, Zheng-wu; Qi, Rui; Li, Xiao-fang; Liu, Zhi-xiong

    2014-10-25

    Arabidopsis thaliana APETALA3 (AP3) and Antirrhinum majus DEFICIENS (DEF) MADS box genes are required to specify petal and stamen identity. AP3 and DEF are members of the euAP3 lineage, which arose by gene duplication coincident with radiation of the core eudicots. In order to investigate the molecular mechanisms underlying organ development in early diverging clades of core eudicots, we isolated and identified an AP3 homolog, FaesAP3, from Fagopyrum esculentum (buckwheat, Polygonaceae), a multi-food-use pseudocereal with healing benefits. Protein sequence alignment and phylogenetic analyses revealed that FaesAP3 grouped into the euAP3 lineage. Expression analysis showed that FaesAP3 was transcribed only in developing stamens, and differed from AP3 and DEF, which expressed in developing petals and stamens. Moreover, ectopic expression of FaesAP3 rescued stamen development without complementation of petal development in an Arabidopsis ap3 mutant. Our results suggest that FaesAP3 is involved in the development of stamens in buckwheat. These results also suggest that FaesAP3 holds some potential for biotechnical engineering to create a male sterile line of F. esculentum. PMID:25149019

  8. The AP1000 reactor

    International Nuclear Information System (INIS)

    The design of the AP1000 reactor began 20 years ago when Westinghouse launched the AP600 reactor project. In fact by re-assessing AP600's safety margins Westinghouse realized that the its power output could be raised without putting at risk its safety standard. The AP1000 was born, it yields 1100 MWe. The main AP1000's design features is its passive safety (particularly after the Fukushima accident) and its modularity. The passive safety of the AP1000 implies: -) no humane intervention needed for 72 hours at least after the incident; -) no necessity for redundant complex safety systems. The modularity means that the plant, the reactor and other buildings are constructed from a choice of 300 modular units. These units can be built off-site and fit together on site. The modularity allows more construction activities to be led simultaneously and more chances to cope with the construction schedule. The NRC has approved the operation license for 30 years of the first AP1000 being built in the Usa (Vogtle plant in Georgia). 4 AP1000 are being built in China (Sanmen and Haiyang sites) and 6 others are planned in the Usa. Westinghouse is convinced that the AP1000's passive safety makes it more attractive. Let us not forget that Westinghouse was at the origin of the concept of pressurized water reactors, an idea adopted for half the nuclear power stations in the world and for all the plants now active in France. (A.C.)

  9. On-line laser spectroscopy with a cooled He-jet transport system

    International Nuclear Information System (INIS)

    In order to make nuclear size and shape determinations for short-lived nuclei, we are using a transport system with pure, cooled helium to carry radioactive atoms produced by heavy-ion reactions from the target chamber into a laser-spectroscopy chamber. The device is attached to a beam line of the Argonne Superconducting Heavy-Ion Linac. Upon leaving the transport system, the atoms form a cooled atomic beam suitable for several high-sensitivity, high-precision spectroscopic techniques. We plan to use the photon-burst method which permits high-resolution hyperfine structure measurements with only a few atoms per second. The efficiency of the helium-jet system, the extension of the photon-burst method to slow and optically-pumped transitions, and the present status of the project are discussed

  10. Road and Street Centerlines - RAMPS_INDOT_IN: Ramp System in Indiana (Indiana Department of Transportation, Line Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — RAMPS_INDOT_IN is a line shapefile that contains all ramps in Indiana, provided by personnel of Indiana Department of Transportation (INDOT), Business Information...

  11. Design of the low energy beam transport line for the China spallation neutron source

    Institute of Scientific and Technical Information of China (English)

    LI Jin-Hai; OUYANG Hua-Fu; FU Shi-Nian; ZHANG Sua-Shun; HE Wei

    2008-01-01

    The design of the China Spallation Neutron Source (CSNS) low-energy beam transport (LEBT) line, which locates between the ion source and the radio-frequency quadrupole (RFQ), has been completed with the TRACE3D code. The design aims at perfect matching, primary chopping, a small emittance growth and sufficient space for beam diagnostics. The line consists of three solenoids, three vacuum chambers, two steering magnets and a pre-chopper. The total length of LEBT is about 1.74 m. This LEBT is designed to transfer 20 mA of H-pulsed beam from the ion source to the RFQ. An induction cavity is adopted as the pre-chopper.The electrostatic octupole steerer is discussed as a candidate. A four-quadrant aperture for beam scraping and beam position monitoring is designed.

  12. Survey and alignment of high energy physics accelerators and transport lines

    International Nuclear Information System (INIS)

    This talk summarizes the survey and alignment processes of accelerators and transport lines and discusses the propagation of errors associated with these processes. The major geodetic principles governing the survey and alignment measurement space are revisited and their relationship to a lattice coordinate system shown. The paper continues with a broad overview about the activities involved in the step by step sequence from initial absolute alignment to final smoothing. Emphasis is given to the relative alignment of components, in particular to the importance of incorporating methods to remove residual systematic effects in surveying and alignment operations

  13. Line photon transport in a non-homogeneous plasma using radiative coupling coefficients

    Energy Technology Data Exchange (ETDEWEB)

    Florido, R.; Gil, J.M.; Rodriguez, R.; Rubiano, J.G.; Martel, P. [Las Palmas de Gran Canaria Univ., Dept. de Fisica (Spain); Florido, R.; Gil, J.M.; Rodriguez, R.; Rubiano, J.G.; Martel, P.; Minguez, E. [Madrid Univ. Politecnica, Instituto de Fusion Nuclear-DENIM (Spain)

    2006-06-15

    We present a steady-state collisional-radiative model for the calculation of level populations in non-homogeneous plasmas with planar geometry. The line photon transport is taken into account following an angle- and frequency-averaged escape probability model. Several models where the same approach has been used can be found in the literature, but the main difference between our model and those ones is that the details of geometry are exactly treated in the definition of coupling coefficients and a local profile is taken into account in each plasma cell. (authors)

  14. Reversal of resistance by GF120918 in cell lines expressing the ABC half-transporter, MXR

    DEFF Research Database (Denmark)

    de Bruin, M; Miyake, K; Litman, Thomas;

    1999-01-01

    assays, rhodamine and calcein efflux assays, and confocal microscopy in cell lines expressing different multidrug resistance transporters. At a concentration of 1 microM in cytotoxicity assays, GF120918 was able to sensitize both S1-B1-20, a subline expressing P-glycoprotein (Pgp), and S1-M1...... concentrations as low as 25-50 nM, with 250 nM giving complete inhibition of rhodamine efflux. Complete inhibition of rhodamine efflux in mitoxantrone-resistant S1-M1-80 cells required 10 microM. Examination of intracellular mitoxantrone accumulation by confocal microscopy confirmed higher levels of mitoxantrone...

  15. Functional Coding Variation in Recombinant Inbred Mouse Lines Reveals Novel Serotonin Transporter-Associated Phenotypes

    Energy Technology Data Exchange (ETDEWEB)

    Carneiro, Ana [Vanderbilt University; Airey, David [University of Tennessee Health Science Center, Memphis; Thompson, Brent [Vanderbilt University; Zhu, C [Vanderbilt University; Rinchik, Eugene M [ORNL; Lu, Lu [University of Tennessee Health Science Center, Memphis; Chesler, Elissa J [ORNL; Erikson, Keith [University of North Carolina; Blakely, Randy [Vanderbilt University

    2009-01-01

    The human serotonin (5-hydroxytryptamine, 5-HT) transporter (hSERT, SLC6A4) figures prominently in the etiology or treatment of many prevalent neurobehavioral disorders including anxiety, alcoholism, depression, autism and obsessive-compulsive disorder (OCD). Here we utilize naturally occurring polymorphisms in recombinant inbred (RI) lines to identify novel phenotypes associated with altered SERT function. The widely used mouse strain C57BL/6J, harbors a SERT haplotype defined by two nonsynonymous coding variants (Gly39 and Lys152 (GK)). At these positions, many other mouse lines, including DBA/2J, encode Glu39 and Arg152 (ER haplotype), assignments found also in hSERT. Synaptosomal 5-HT transport studies revealed reduced uptake associated with the GK variant. Heterologous expression studies confirmed a reduced SERT turnover rate for the GK variant. Experimental and in silico approaches using RI lines (C57Bl/6J X DBA/2J=BXD) identifies multiple anatomical, biochemical and behavioral phenotypes specifically impacted by GK/ER variation. Among our findings are multiple traits associated with anxiety and alcohol consumption, as well as of the control of dopamine (DA) signaling. Further bioinformatic analysis of BXD phenotypes, combined with biochemical evaluation of SERT knockout mice, nominates SERT-dependent 5-HT signaling as a major determinant of midbrain iron homeostasis that, in turn, dictates ironregulated DA phenotypes. Our studies provide a novel example of the power of coordinated in vitro, in vivo and in silico approaches using murine RI lines to elucidate and quantify the system-level impact of gene variation.

  16. Generic effluent monitoring system certification for AP-40 exhauster stack

    Energy Technology Data Exchange (ETDEWEB)

    Glissmeyer, J.A.; Davis, W.E.; Bussell, J.H.; Maughan, A.D.

    1997-09-01

    Tests were conducted to verify that the Generic Effluent Monitoring System (GEMS), as applied to the AP-40 exhauster stack, meets all applicable regulatory performance criteria for air sampling systems at nuclear facilities. These performance criteria address both the suitability of the air sampling probe location and the transport of the sample to the collection devices. The criteria covering air sampling probe location ensure that the contaminants in the stack are well mixed with the airflow at the probe location such that the extracted sample represents the whole. The sample transport criteria ensure that the sampled contaminants are quantitatively delivered to the collection device. The specific performance criteria are described in detail in the report. The tests demonstrated that the GEMS/AP-40 system meets all applicable performance criteria. The contaminant mixing tests were conducted by Pacific Northwest National Laboratory (PNNL) at the wind tunnel facility, 331-H Building, using a mockup of the actual stack. The particle sample transport tests were conducted by PNNL at the Numatec Hanford Company`s 305 Building. The AP-40 stack is typical of several 10-in. diameter stacks that discharge the filtered ventilation air from tank farms at the U.S. Department of Energy`s Hanford Site in Richland, Washington. The GEMS design features a probe with a single shrouded sampling nozzle, a sample delivery line, and sample collection system. The collection system includes a filter holder to collect the sample of record and an in-line detector head and filter for monitoring beta radiation-emitting particles. Unrelated to the performance criteria, it was found that the record sample filter holder exhibited symptoms of sample bypass around the particle collection filter. This filter holder should either be modified or replaced with a different type. 10 refs., 8 figs., 6 tabs.

  17. Dietary and Developmental Regulation of Nutrient Transporter Gene Expression in the Small Intestine of Two Lines of Broilers

    OpenAIRE

    Gilbert, Elizabeth Ruth

    2008-01-01

    To better understand the digestive and absorptive capacities of the chick intestine so that we may feed diets that better meet the nutritional needs of the chick, it is important to understand how expression of nutrient transporter genes changes in response to various factors. A series of feeding trials were conducted to evaluate the dietary and developmental regulation of nutrient transporter mRNA abundance in the small intestine of two lines of broilers selected on corn-based (Line A) or wh...

  18. Characterization of a murine renal distal convoluted tubule cell line for the study of transcellular calcium transport

    NARCIS (Netherlands)

    Diepens, R.J.W.; Dekker, E. den; Bens, M.; Weidema, A.F.; Walle, A. van de; Bindels, R.J.M.; Hoenderop, J.G.J.

    2004-01-01

    To unravel the molecular regulation of renal transcellular Ca(2+) transport, a murine distal convoluted tubule (mpkDCT) cell line derived from distal convoluted tubules (DCT) microdissected from a SV-PK/Tag transgenic mouse was characterized. This cell line originated from DCT only, as mRNA encoding

  19. Reconstitution of bile acid transport in the rat hepatoma McArdle RH-7777 cell line.

    Science.gov (United States)

    Torchia, E C; Shapiro, R J; Agellon, L B

    1996-07-01

    The liver recovers bile acids from the portal circulation primarily via an active process that is dependent on sodium ions. Hepatocytes lose the ability to transport bile acids in culture, and, in liver-derived permanent cell lines, this ability is severely reduced or absent. To study the importance of bile acids in regulating liver-specific functions (e.g., cellular bile acid and cholesterol metabolism), we have re-established active bile acid transport in cultured cells. The complementary DNA (cDNA) encoding the rat sodium/taurocholate cotransporting polypeptide (ntcp) was placed under the control of a cytomegalovirus promoter and transfected into the rat hepatoma cell line, McArdle RH-7777. Transfected cells were screened for the ability to take up [3H]-taurocholate. Clones that displayed the ability to take up taurocholate were expanded (designated McNtcp) and further characterized. The apparent Michaelis constant (Km) for taurocholate uptake was similar among the different clones. The observed maximum velocity (Vmax), however, differed and was positively correlated with the abundance of recombinant ntcp messenger RNA (mRNA). The highest level of taurocholate uptake activity observed in McNtcp cells was comparable with that of freshly isolated hepatocytes. Efflux of accumulated taurocholate from McNtcp cells proceeded in a manner similar to primary hepatocytes, indicating that McArdle RH-7777 cells have retained the ability to secrete bile acids. Moreover, taurocholate uptake in McNtcp cells was inhibited by other bile acid species. Based on the observed kinetic parameters, the reconstituted McArdle RH-7777 cells mimic the ability of primary hepatocytes to transport bile acids.

  20. In vitro evaluation on pharmacokinetics using a cell line expressing human renal transporters

    Institute of Scientific and Technical Information of China (English)

    HuanXL; EndoH

    2002-01-01

    To maintain the normal physiological ionic environment,renal transporters specialize in removing waste materials,such as toxic substances,drugs and other organic cations and anions from the blood in a process of renal secretion.The purpose of this study was focused on characterizing molecular mechanisms and pharmacokinetics in renal elimination of various compounds.In this study,the development of the proximal tubule cell (S2 cell) lines stably expressing human organicanion (hOAT1,hOAT2,hOAT3 and hOAT4) and cation (hOCT1 and hOCT2) transporters were established and specific affinity of transporter for drugs is being clarified.The drug interaction between various drugs will be evaluated by measuring time- and concentration-dependent uptake and providing an exact Km and Ki value.In this presentation,the study methodology and its application using model compounds such as [3H] riboflavin,[3H] diazepam and [3H] clonidine will be shown.

  1. Optimization of solenoid based low energy beam transport line for high current H+ beams

    Science.gov (United States)

    Pande, R.; Singh, P.; Rao, S. V. L. S.; Roy, S.; Krishnagopal, S.

    2015-02-01

    A 20 MeV, 30 mA CW proton linac is being developed at BARC, Mumbai. This linac will consist of an ECR ion source followed by a Radio Frequency Quadrupole (RFQ) and Drift tube Linac (DTL). The low energy beam transport (LEBT) line is used to match the beam from the ion source to the RFQ with minimum beam loss and increase in emittance. The LEBT is also used to eliminate the unwanted ions like H2+ and H3+ from entering the RFQ. In addition, space charge compensation is required for transportation of such high beam currents. All this requires careful design and optimization. Detailed beam dynamics simulations have been done to optimize the design of the LEBT using the Particle-in-cell code TRACEWIN. We find that with careful optimization it is possible to transport a 30 mA CW proton beam through the LEBT with 100% transmission and minimal emittance blow up, while at the same time suppressing unwanted species H2+ and H3+ to less than 3.3% of the total beam current.

  2. Noble internal transport barriers and radial subdiffusion of toroidal magnetic lines

    Energy Technology Data Exchange (ETDEWEB)

    Misguich, J.H.; Reuss, J.D. [Association Euratom-CEA sur la Fusion, CEA/DSM/DRFC, 13 - Saint Paul lez Durance (France); Constantinescu, D.; Steinbrecher, G. [Association Euratom-N.A.S.T.I., Dept. of Physics, University of Craiova (Romania); Vlad, M.; Spineanu, F. [Association Euratom-N.A.S.T.I., National Institute of Laser, Plasma and Radiation Physics, Bucharest (Romania); Weyssow, B.; Balescu, R. [Association Euratom-Etat Belge sur la Fusion, Universite Libre de Bruxelles (Belgium)

    2002-02-01

    Internal transport barriers (ITB's) observed in tokamaks are described by a purely magnetic approach. Magnetic line motion in toroidal geometry with broken magnetic surfaces is studied from a previously derived Hamiltonian map in situation of incomplete chaos. This appears to reproduce in a realistic way the main features of a tokamak, for a given safety factor profile and in terms of a single parameter L representing the amplitude of the magnetic perturbation. New results are given concerning the Shafranov shift as function of L. For small values of L, closed magnetic surfaces exist (KAM tori) and island chains begin to appear on rational surfaces for higher values of L, with chaotic zones around hyperbolic points, as expected. Single trajectories of magnetic line motion indicate the persistence of a central protected plasma core, surrounded by a chaotic shell enclosed in a double-sided transport barrier. Magnetic lines which succeed to escape across this barrier begin to wander in a wide chaotic sea extending up to a very robust barrier (as long as L{<=}1). For values of L{>=}1, above the escape threshold, most magnetic lines succeed to escape out of the external barrier which has become a permeable Cantorus. Statistical analysis of a large number of trajectories, representing the evolution of a bunch of magnetic lines, indicate that the flux variable {psi} asymptotically grows in a diffuse manner as (L{sup 2}t) with a L{sup 2} scaling as expected, but that the average radial position r{sub m}(t) asymptotically grows as (L{sup 2}t){sup 1/4} while the mean square displacement around this average radius asymptotically grows in a sub-diffusive manner as (L{sup 2}t){sup 1/2}. This result shows the slower dispersion in the present incomplete chaotic regime, which is different from the usual quasilinear diffusion in completely chaotic situations. For physical times t{sub {phi}} of the order of the escape time defined by x{sub m}(t{sub {phi}}) {approx}1, the motion

  3. The effects of magnetic fringe fields on beam dynamics in a beam transport line of a terahertz FEL source

    Energy Technology Data Exchange (ETDEWEB)

    Zeng, Han [State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei (China); Xiong, Yongqian, E-mail: yqxiong@mail.hust.edu.cn [State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei (China); Pei, Yuanji [National Synchrotron Radiation laboratory, University of Science and Technology of China, Hefei 230029, Anhui (China)

    2014-11-11

    The transport line used in a terahertz FEL device has to transport electron beam through the entire system efficiently and meet the requirements of the beam parameters at the undulator entrance. Due to space limitations, the size of the magnets (five quadrupoles and two bending magnets) employed in the transport line was limited, and some devices were densely packed. In this paper, analyses of the effect of fringe fields and magnetic interference of magnets are presented. 3D models of these magnets are built and their linear optical properties are compared with those obtained by hard edge models. The results indicated that the effects of these factors are significant and they would cause a mismatch of the beam at the exit of the transport line under the preliminary lattice design. To solve this problem, the beam was re-matched using the particle swarm optimization algorithm.

  4. APS Science 2006.

    Energy Technology Data Exchange (ETDEWEB)

    Gibson, J. M.; Fenner, R. B.; Long, G.; Borland, M.; Decker, G.

    2007-05-24

    In my five years as the Director of the Advanced Photon Source (APS), I have been fortunate to see major growth in the scientific impact from the APS. This year I am particularly enthusiastic about prospects for our longer-term future. Every scientific instrument must remain at the cutting edge to flourish. Our plans for the next generation of APS--an APS upgrade--got seriously in gear this year with strong encouragement from our users and sponsors. The most promising avenue that has emerged is the energy-recovery linac (ERL) (see article on page xx), for which we are beginning serious R&D. The ERL{at}APS would offer revolutionary performance, especially for x-ray imaging and ultrafast science, while not seriously disrupting the existing user base. I am very proud of our accelerator physics and engineering staff, who not only keep the current APS at the forefront, but were able to greatly impress our international Machine Advisory Committee with the quality of their work on the possible upgrade option (see page xx). As we prepare for long-term major upgrades, our plans to develop and optimize all the sectors at APS in the near future are advancing. Several new beamlines saw first light this year, including a dedicated powder diffraction beamline (11-BM), two instruments for inelastic x-ray scattering at sector 30, and the Center for Nanoscale Materials (CNM) Nanoprobe beamline at sector 26. Our partnership in the first x-ray free-electron laser (LCLS) to be built at Stanford contributes to revolutionary growth in ultrafast science (see page xx), and we are developing a pulse chirping scheme to get ps pulses at sector 7 of the APS within a year or so. In this report, you will find selected highlights of scientific research at the APS from calendar year 2006. The highlighted work covers diverse disciplines, from fundamental to applied science. In the article on page xx you can see the direct impact of APS research on technology. Several new products have emerged from

  5. AP statistics crash course

    CERN Document Server

    D'Alessio, Michael

    2012-01-01

    AP Statistics Crash Course - Gets You a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. AP Statistics Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know Crash Course is based on an in-depth analysis of the AP Statistics course description outline and actual Advanced Placement test questions. It covers only the information tested on the exam, so you can make the most of your valuable study time. Our easy-to-read format covers: exploring da

  6. Quantitative assessment of direct and indirect landslide risk along transportation lines in southern India

    Science.gov (United States)

    Jaiswal, P.; van Westen, C. J.; Jetten, V.

    2010-06-01

    A quantitative approach for landslide risk assessment along transportation lines is presented and applied to a road and a railway alignment in the Nilgiri hills in southern India. The method allows estimating direct risk affecting the alignments, vehicles and people, and indirect risk resulting from the disruption of economic activities. The data required for the risk estimation were obtained from historical records. A total of 901 landslides were catalogued initiating from cut slopes along the railway and road alignment. The landslides were grouped into three magnitude classes based on the landslide type, volume, scar depth, run-out distance, etc and their probability of occurrence was obtained using frequency-volume distribution. Hazard, for a given return period, expressed as the number of landslides of a given magnitude class per kilometre of cut slopes, was obtained using Gumbel distribution and probability of landslide magnitude. In total 18 specific hazard scenarios were generated using the three magnitude classes and six return periods (1, 3, 5, 15, 25, and 50 years). The assessment of the vulnerability of the road and railway line was based on damage records whereas the vulnerability of different types of vehicles and people was subjectively assessed based on limited historic incidents. Direct specific loss for the alignments (railway line and road), vehicles (train, bus, lorry, car and motorbike) was expressed in monetary value (US), and direct specific loss of life of commuters was expressed in annual probability of death. Indirect specific loss (US) derived from the traffic interruption was evaluated considering alternative driving routes, and includes losses resulting from additional fuel consumption, additional travel cost, loss of income to the local business, and loss of revenue to the railway department. The results indicate that the total loss, including both direct and indirect loss, from 1 to 50 years return period, varies from US 90 840 to US

  7. THE COMMISSIONING PLAN FOR THE SPALLATION NEUTRON SOURCE RING AND TRANSPORT LINES.

    Energy Technology Data Exchange (ETDEWEB)

    RAPARIA,D.BLASKIEWICZ,M.LEE,Y.Y.WEI,J.ET AL.

    2004-03-10

    The Spallation Neutron Source (SNS) accelerator systems will provide a 1 GeV, 1.44 MW proton beam to a liquid mercury target for neutron production. In order to satisfy the accelerator systems' portion of the Critical Decision 4 (CD-4) commissioning goal (which marks the completion of the construction phase of the project), a beam pulse with intensity greater than 1 x 10{sup 13} protons must be accumulated in the ring, extracted in a single turn and delivered to the target. A commissioning plan has been formulated for bringing into operation and establishing nominal operating conditions for the various ring and transport line subsystems as well as for establishing beam conditions and parameters which meet the commissioning goal.

  8. Comparison of implicit and symbolic implicit Monte Carlo line transport with frequency weight vector extension

    Science.gov (United States)

    McKinley, Michael Scott; Brooks, Eugene D., III; Szoke, Abraham

    2003-07-01

    We compare the implicit Monte Carlo (IMC) technique to the symbolic IMC (SIMC) technique, with and without weight vectors in frequency space, for time-dependent line transport in the presence of collisional pumping. We examine the efficiency and accuracy of the IMC and SIMC methods for test problems involving the evolution of a collisionally pumped trapping problem to its steady-state, the surface heating of a cold medium by a beam, and the diffusion of energy from a localized region that is collisionally pumped. The importance of spatial biasing and teleportation for problems involving high opacity is demonstrated. Our numerical solution, along with its associated teleportation error, is checked against theoretical calculations for the last example.

  9. Lattice design of medium energy beam transport line for n spallation neutron source

    International Nuclear Information System (INIS)

    A 1 GeV H- injector linac is being designed at RRCAT for the proposed Indian Spallation Neutron Source (ISNS). The front-end of the injector linac will consist of Radiofrequency Quadrupole (RFQ) linac, which will accelerate the H- beam from 50 keV to 3 MeV. The beam will be further accelerated in superconducting Single Spoke Resonators (SSRs). A Medium Energy Beam Transport (MEBT) line will be used to transport the beam from the exit of RFQ to the input of SSR. The main purpose of MEBT is to carry out beam matching from RFQ to SSR, and beam chopping. In this paper, we describe the optimization criteria for the lattice design of MEBT. The optimized lattice element parameters are presented for zero and full (15 mA) current case. Beam dynamics studies have been carried out using an envelope tracing code Trace-3D. Required beam deflection angle due to the chopper housed inside the MEBT for beam chopping has also been estimated. (author)

  10. APS Science 2009

    International Nuclear Information System (INIS)

    It is my pleasure to introduce the 2009 annual report of the Advanced Photon Source. This was a very good year for us. We operated with high reliability and availability, despite growing problems with obsolete systems, and our users produced a record output of publications. The number of user experiments increased by 14% from 2008 to more than 3600. We congratulate the recipients of the 2009 Nobel Prize in Chemistry-Venkatraman Ramakrishnan (Cambridge Institute for Medical Research), Thomas Steitz (Yale University), and Ada Yonath (Weizmann Institute) - who did a substantial amount of this work at APS beamlines. Thanks to the efforts of our users and staff, and the ongoing counsel of the APS Scientific Advisory Committee, we made major progress in advancing our planning for the upgrade of the APS (APS-U), producing a proposal that was positively reviewed. We hope to get formal approval in 2010 to begin the upgrade. With advocacy from our users and the support of our sponsor, the Office of Basic Energy Sciences in the Department of Energy (DOE) Office of Science, our operating budgets have grown to the level needed to more adequately staff our beamlines. We were also extremely fortunate to have received $7.9 M in American Recovery and Reinvestment Act ('stimulus') funding to acquire new detectors and improve several of our beamlines. The success of the new Linac Coherent Light Source at Stanford, the world's first x-ray free-electron laser, made us particularly proud since the undulators were designed and built by the APS. Among other highlights, we note that more than one-quarter of the 46 Energy Frontier Research Centers, funded competitively across the U.S. in 2009 by the DOE, included the Advanced Photon Source in their proposed work, which shows that synchrotron radiation, and the APS in particular, are central to energy research. While APS research covers everything from fundamental to applied science (reflected by the highlights in this report), the challenge

  11. APS Science 2009.

    Energy Technology Data Exchange (ETDEWEB)

    Gibson, J. M; Mills, D. M.; Gerig, R.

    2010-05-01

    It is my pleasure to introduce the 2009 annual report of the Advanced Photon Source. This was a very good year for us. We operated with high reliability and availability, despite growing problems with obsolete systems, and our users produced a record output of publications. The number of user experiments increased by 14% from 2008 to more than 3600. We congratulate the recipients of the 2009 Nobel Prize in Chemistry-Venkatraman Ramakrishnan (Cambridge Institute for Medical Research), Thomas Steitz (Yale University), and Ada Yonath (Weizmann Institute) - who did a substantial amount of this work at APS beamlines. Thanks to the efforts of our users and staff, and the ongoing counsel of the APS Scientific Advisory Committee, we made major progress in advancing our planning for the upgrade of the APS (APS-U), producing a proposal that was positively reviewed. We hope to get formal approval in 2010 to begin the upgrade. With advocacy from our users and the support of our sponsor, the Office of Basic Energy Sciences in the Department of Energy (DOE) Office of Science, our operating budgets have grown to the level needed to more adequately staff our beamlines. We were also extremely fortunate to have received $7.9 M in American Recovery and Reinvestment Act ('stimulus') funding to acquire new detectors and improve several of our beamlines. The success of the new Linac Coherent Light Source at Stanford, the world's first x-ray free-electron laser, made us particularly proud since the undulators were designed and built by the APS. Among other highlights, we note that more than one-quarter of the 46 Energy Frontier Research Centers, funded competitively across the U.S. in 2009 by the DOE, included the Advanced Photon Source in their proposed work, which shows that synchrotron radiation, and the APS in particular, are central to energy research. While APS research covers everything from fundamental to applied science (reflected by the highlights in this report

  12. Adaptor protein complexes and intracellular transport

    OpenAIRE

    2014-01-01

    The AP (adaptor protein) complexes are heterotetrameric protein complexes that mediate intracellular membrane trafficking along endocytic and secretory transport pathways. There are five different AP complexes: AP-1, AP-2 and AP-3 are clathrin-associated complexes; whereas AP-4 and AP-5 are not. These five AP complexes localize to different intracellular compartments and mediate membrane trafficking in distinct pathways. They recognize and concentrate cargo proteins into vesicular carriers th...

  13. Parametric study of transport beam lines for electron beams accelerated by laser-plasma interaction

    Science.gov (United States)

    Scisciò, M.; Lancia, L.; Migliorati, M.; Mostacci, A.; Palumbo, L.; Papaphilippou, Y.; Antici, P.

    2016-03-01

    In the last decade, laser-plasma acceleration of high-energy electrons has attracted strong attention in different fields. Electrons with maximum energies in the GeV range can be laser-accelerated within a few cm using multi-hundreds terawatt (TW) lasers, yielding to very high beam currents at the source (electron bunches with up to tens-hundreds of pC in a few fs). While initially the challenge was to increase the maximum achievable electron energy, today strong effort is put in the control and usability of these laser-generated beams that still lack of some features in order to be used for applications where currently conventional, radio-frequency (RF) based, electron beam lines represent the most common and efficient solution. Several improvements have been suggested for this purpose, some of them acting directly on the plasma source, some using beam shaping tools located downstream. Concerning the latter, several studies have suggested the use of conventional accelerator magnetic devices (such as quadrupoles and solenoids) as an easy implementable solution when the laser-plasma accelerated beam requires optimization. In this paper, we report on a parametric study related to the transport of electron beams accelerated by laser-plasma interaction, using conventional accelerator elements and tools. We focus on both, high energy electron beams in the GeV range, as produced on petawatt (PW) class laser systems, and on lower energy electron beams in the hundreds of MeV range, as nowadays routinely obtained on commercially available multi-hundred TW laser systems. For both scenarios, our study allows understanding what are the crucial parameters that enable laser-plasma accelerators to compete with conventional ones and allow for a beam transport. We show that suitable working points require a tradeoff-combination between low beam divergence and narrow energy spread.

  14. Characterization of a mutation and an alternative splicing of UDP-galactose transporter in MDCK-RCAr cell line.

    Science.gov (United States)

    Olczak, Mariusz; Guillen, Eduardo

    2006-01-01

    The UDP-galactose (UDP-Gal) transporter present in the Golgi apparatus is a member of a transporter family comprising hydrophobic proteins with multiple transmembrane domains. Co-immunoprecipitation experiments showed that the full-length UDP-Gal transporter protein forms oligomeric structures in the MDCK cell. A ricin-resistant mutant of the MDCK cell line (MDCK-RCA(r)) is deficient in galactose linked to macromolecules because of a lower UDP-Gal transport rate into the Golgi apparatus. We cloned this mutated protein and found that it contains a stop codon close to the 5' terminus of its open reading frame. We also detected a shorter splicing variant of the UDP-Gal transporter which contains a 183-nt in-frame deletion in both the wild-type and the mutant mRNA. We showed that the protein, when overexpressed, is localized in the Golgi apparatus and could partially correct the phenotype of the MDCK-RCA(r) and CHO-Lec8 mutant cell lines. The level of mRNA of the UDP-Gal transporter is much lower (25-30 copies per cell) than those of the CMP-sialic acid transporter (100 copies per cell), UDP-N-acetylglucosamine transporter (80 copies per cell), and GDP-fucose transporter (65 copies per cell). The transcript level of the shorter splicing variant of the UDP-Gal transporter is extremely rare in wild-type MDCK cells (a few copies per cell), but it is significantly increased in the mutant, RCA-resistant cells. PMID:16434112

  15. Differential expression of several drug transporter genes in HepG2 and Huh-7 cell lines

    Science.gov (United States)

    Louisa, Melva; Suyatna, Frans D.; Wanandi, Septelia Inawati; Asih, Puji Budi Setia; Syafruddin, Din

    2016-01-01

    Background: Cell culture techniques have many advantages for investigation of drug transport to target organ like liver. HepG2 and Huh-7 are two cell lines available from hepatoma that can be used as a model for hepatic drug transport. The present study is aimed to analyze the expression level of several drug transporter genes in two hepatoma cell lines, HepG2 and Huh-7 and their response to inhibitors. Materials and Methods: This is an in vitro study using HepG2 and Huh-7 cells. The expression level of the following drug transporter genes was quantified: P-glycoprotein/multidrug resistance protein 1, Organic Anionic Transporter Protein 1B1 (OATP1B1) and Organic Cationic Transporter-1 (OCT1). Ribonucleic acid was extracted from the cells using Tripure isolation reagent, then gene expression level of the transporters is quantified using Applied Biosystems quantitative reverse transcriptase polymerase chain reaction. Verapamil (P-glycoprotein inhibitor), nelfinavir (OATP1B1 inhibitor), quinidine (OCT1 inhibitor) were used to differentiate the inhibitory properties of these agents to the transporter expressions in HepG2 and Huh-7 cells. Results: Huh-7 shows a higher level of P-glycoprotein, OATP1B1 and OCT1 expressions compared with those of HepG2. Verapamil reduces the expressions of P-glycoprotein in HepG2 and Huh-7; nelfinavir reduces the expression of OATP1B1 in HepG2 and Huh-7; while quinidine reduces the OCT1 gene expressions in HepG2, but not in Huh-7 cells. Conclusion: This study indicates that HepG2 might be a more suitable in vitro model than Huh-7 to study drug transport in hepatocytes involving drug transporters. PMID:27376043

  16. Improved design of proton source and low energy beam transport line for European Spallation Source

    Energy Technology Data Exchange (ETDEWEB)

    Neri, L., E-mail: neri@lns.infn.it; Celona, L.; Gammino, S.; Mascali, D.; Castro, G.; Ciavola, G. [Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, Via S. Sofia 62, 95123 Catania (Italy); Torrisi, G. [Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, Via S. Sofia 62, 95123 Catania (Italy); Dipartimento di Ingegneria dell’Informazione, delle Infrastrutture e dell’Energia Sostenibile, Università Mediterranea di Reggio Calabria, Via Graziella, 89122 Reggio Calabria (Italy); Cheymol, B.; Ponton, A. [European Spallation Source ESS AB, Lund (Sweden); Galatà, A. [Laboratori Nazionali di Legnaro, Istituto Nazionale di Fisica Nucleare, Viale dell' università 2, 35020 Legnaro (Italy); Patti, G. [Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, Via S. Sofia 62, 95123 Catania (Italy); Laboratori Nazionali di Legnaro, Istituto Nazionale di Fisica Nucleare, Viale dell' università 2, 35020 Legnaro (Italy); Gozzo, A.; Lega, L. [Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, Via S. Sofia 62, 95123 Catania (Italy); Dipartimento di Ingegneria Informatica e delle Telecomunicazioni, Università degli Studi di Catania, Viale Andrea Doria 6, 95123 Catania (Italy)

    2014-02-15

    The design update of the European Spallation Source (ESS) accelerator is almost complete and the construction of the prototype of the microwave discharge ion source able to provide a proton beam current larger than 70 mA to the 3.6 MeV Radio Frequency Quadrupole (RFQ) started. The source named PS-ESS (Proton Source for ESS) was designed with a flexible magnetic system and an extraction system able to merge conservative solutions with significant advances. The ESS injector has taken advantage of recent theoretical updates and new plasma diagnostics tools developed at INFN-LNS (Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare). The design strategy considers the PS-ESS and the low energy beam transport line as a whole, where the proton beam behaves like an almost neutralized non-thermalized plasma. Innovative solutions have been used as hereinafter described. Thermo-mechanical optimization has been performed to withstand the chopped beam and the misaligned focused beam over the RFQ input collimator; the results are reported here.

  17. Improved design of proton source and low energy beam transport line for European Spallation Source

    Science.gov (United States)

    Neri, L.; Celona, L.; Gammino, S.; Mascali, D.; Castro, G.; Torrisi, G.; Cheymol, B.; Ponton, A.; Galatà, A.; Patti, G.; Gozzo, A.; Lega, L.; Ciavola, G.

    2014-02-01

    The design update of the European Spallation Source (ESS) accelerator is almost complete and the construction of the prototype of the microwave discharge ion source able to provide a proton beam current larger than 70 mA to the 3.6 MeV Radio Frequency Quadrupole (RFQ) started. The source named PS-ESS (Proton Source for ESS) was designed with a flexible magnetic system and an extraction system able to merge conservative solutions with significant advances. The ESS injector has taken advantage of recent theoretical updates and new plasma diagnostics tools developed at INFN-LNS (Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare). The design strategy considers the PS-ESS and the low energy beam transport line as a whole, where the proton beam behaves like an almost neutralized non-thermalized plasma. Innovative solutions have been used as hereinafter described. Thermo-mechanical optimization has been performed to withstand the chopped beam and the misaligned focused beam over the RFQ input collimator; the results are reported here.

  18. The programme library for numerical simulation of charged particle dynamics in transportation lines

    International Nuclear Information System (INIS)

    The description of a PC codes library to simulate the beam transportation of charged particles is presented. The codes are realized on IBM PC in Visual Basic common interface. It is destined for the simulation and optimization of beam dynamics and based on the successive and consistent use of two methods: the momentum method of distribution functions (RMS technique) and the particle-particle method (PP-Method). The library allows to calculate the RMS parameters of electron and ion beams, passing through a set of quadrupoles, solenoids, bends, accelerating sections. The RMS code is a fast code very suitable for the first test, design and optimization of the beam line parameters. The PP code requires more time for execution but provides a high accuracy of simulation taking into account the space charge effects, aberrations and beam losses. One of the main advantages of PP code presented here is an ability to simulate a real multicomponent beam of different masses and charged states of ions from ion sources

  19. Beam Diagnostics Instrumentation for the High Energy Beam Transport Line of I.P.H.I.

    CERN Document Server

    Ausset, P; Coacolo, J L; Lesrel, J; Maymon, J N; Olivier, A; Rouviere, N; Solal-Cohen, M; Vatrinet, L; Yaniche, J F

    2005-01-01

    I.P.H.I. is a High Intensity Proton Injector under construction at Saclay (C.N.R.S/ I.N.2P.3; C.E.A. / D.A.P.N.I.A and C.E.R.N. collaboration). An E.C.R. produces a 100 keV, 100 mA C.W. proton beam which will be accelerated at 3 MeV by a 4 vanes R.F.Q. operating at 352.2 MHz. Finally, a High Energy Beam Transport Line (H.E.B.T.) will deliver the beam to a beam stopper and will be equipped with appropriate beam diagnostics to carry intensity; centroïd beam transverse position, transverse beam profiles, beam energy and energy spread measurements for the commissioning of I.P.H.I. These beam diagnostics will operate under both pulsed and C.W. operation. Transverse beam profile measurements will be acquired under low and high duty factor pulsed beam operation using a slow wire scanner and a C.C.D. camera to image the beam-induced fluorescence. The beam instrumentation of the H.E.B.T. is reviewed and preliminary obtained transverse profile measurements at 100 keV are described.

  20. Orbit Error Correction on the High Energy Beam Transport Line at the KHIMA Accelerator System

    CERN Document Server

    Park, Chawon; Hahn, Garam; An, Dong Hyun

    2016-01-01

    For the purpose of treatment of various cancer and medical research, the synchrotron based medical machine under the Korea Heavy Ion Medical Accelerator (KHIMA) project have been conducted and is going to treat the patient at the beginning of 2018. The KHIMA synchrotron is designed to accelerate and extract the carbon ion (proton) beam with various energy range, 110 up to 430 MeV/u (60 up to 230 MeV). A lattice design and beam optics studies for the High Energy Beam Transport (HEBT) line at the KHIMA accelerator system have been carried out with WinAgile and the MAD-X codes. Because the magnetic eld errors and the mis-alignments introduce to the deviations from the design parameters, these error sources should be treated explicitly and the sensitivity of the machine's lattice to di erent individual error sources is considered. Various types of errors which are static and dynamic one have been taken into account and have been consequentially corrected with a dedicated correction algorithm by using the MAD-X pr...

  1. Effects of line defects on spin-dependent electronic transport of zigzag MoS2 nanoribbons

    Directory of Open Access Journals (Sweden)

    Xin-Mei Li

    2016-01-01

    Full Text Available The nonlinear spin-dependent transport properties in zigzag molybdenum-disulfide nanoribbons (ZMNRs with line defects are investigated systematically using nonequilibrium Green’s function method combined with density functional theory. The results show that the line defects can enhance the electronic transfer ability of ZMNRs. The types and locations of the line defects are found critical in determining the spin polarization and the current-voltage (I-V characteristics of the line defected ZMNRs. For the same defect type, the total currents of the ribbons with the line defects in the centers are lager than those on the edges. And for the same location, the total currents of the systems with the sulfur (S line defect are larger than the according systems with the molybdenum (Mo line defect. All the considered systems present magnetism properties. And in the S line defected systems, the spin reversal behaviors can be observed. In both the spin-up and spin-down states of the Mo line defected systems, there are obvious negative differential resistance behaviors. The mechanisms are proposed for these phenomena.

  2. Effects of line defects on spin-dependent electronic transport of zigzag MoS{sub 2} nanoribbons

    Energy Technology Data Exchange (ETDEWEB)

    Li, Xin-Mei; Yang, Kai-Wei; Zhang, Dan; Ding, Jia-Feng; Xu, Hui, E-mail: xuhui@csu.edu.cn [Institute of Super-microstructure and Ultrafast Process in Advanced Materials & Hunan Key Laboratory for Super-microstructure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha 410083 (China); Long, Meng-Qiu, E-mail: mqlong@csu.edu.cn [Institute of Super-microstructure and Ultrafast Process in Advanced Materials & Hunan Key Laboratory for Super-microstructure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha 410083 (China); Department of Physics and Materials Science, City University of Hong Kong, Hong Kong (China); Cui, Li-Ling [Institute of Super-microstructure and Ultrafast Process in Advanced Materials & Hunan Key Laboratory for Super-microstructure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha 410083 (China); School of Science, Hunan University of Technology, Zhuzhou 412007 (China)

    2016-01-15

    The nonlinear spin-dependent transport properties in zigzag molybdenum-disulfide nanoribbons (ZMNRs) with line defects are investigated systematically using nonequilibrium Green’s function method combined with density functional theory. The results show that the line defects can enhance the electronic transfer ability of ZMNRs. The types and locations of the line defects are found critical in determining the spin polarization and the current-voltage (I-V) characteristics of the line defected ZMNRs. For the same defect type, the total currents of the ribbons with the line defects in the centers are lager than those on the edges. And for the same location, the total currents of the systems with the sulfur (S) line defect are larger than the according systems with the molybdenum (Mo) line defect. All the considered systems present magnetism properties. And in the S line defected systems, the spin reversal behaviors can be observed. In both the spin-up and spin-down states of the Mo line defected systems, there are obvious negative differential resistance behaviors. The mechanisms are proposed for these phenomena.

  3. LINES

    Directory of Open Access Journals (Sweden)

    Minas Bakalchev

    2015-10-01

    Full Text Available The perception of elements in a system often creates their interdependence, interconditionality, and suppression. The lines from a basic geometrical element have become the model of a reductive world based on isolation according to certain criteria such as function, structure, and social organization. Their traces are experienced in the contemporary world as fragments or ruins of a system of domination of an assumed hierarchical unity. How can one release oneself from such dependence or determinism? How can the lines become less “systematic” and forms more autonomous, and less reductive? How is a form released from modernistic determinism on the new controversial ground? How can these elements or forms of representation become forms of action in the present complex world? In this paper, the meaning of lines through the ideas of Le Corbusier, Leonidov, Picasso, and Hitchcock is presented. Spatial research was made through a series of examples arising from the projects of the architectural studio “Residential Transformations”, which was a backbone for mapping the possibilities ranging from playfulness to exactness, as tactics of transformation in the different contexts of the contemporary world.

  4. Correlation of CDK2-AP1 gene over-expression with proliferation and cell cycle regulation of breast cancer cell line MCF-7%乳腺癌MCF-7细胞的增殖及周期与CDK2-AP1基因的表达的相关性研究

    Institute of Scientific and Technical Information of China (English)

    高双全; 高双荣; 肖高芳; 丁宇; 王林辉; 黄伟; 李静

    2013-01-01

    目的 探讨乳腺癌MCF-7细胞的增殖及周期与CDK2-AP1基因的表达的相关性研究,为临床乳腺癌的分子治疗提供基础.方法 取我院研究所保存的人乳腺癌细胞MCF-7进行培养,并构建CDK2-AP1基因编码的病毒表达载体,应用实时定量PCR验证CDK2-AP1基因mRNA和蛋白的表达率.利用流式细胞仪检测MCF-7细胞周期的改变.结果 过表达CDK2-AP1基因的慢病毒感染MCF-7细胞可上调其mRNA表达6.87倍.MCF-7细胞过表达CDK2-AP1基因后,增殖能力显著降低,差异具有统计学意义(P<0.05).流式细胞仪检测证实MCF-7细胞过表达CDK2-AP1能够使细胞周期出现G1期阻滞.结论 CDK2-AP1基因具有抑癌基因的功能,在乳腺癌MCF-7细胞过表达该基因能够抑制细胞的生长和克隆形成能力,并且使细胞阻滞于G1期.

  5. An alternatively spliced mRNA from the AP-2 gene encodes a negative regulator of transcriptional activation by AP-2.

    OpenAIRE

    Buettner, R; Kannan, P; Imhof, A.; Bauer, R.; Yim, S O; Glockshuber, R; Van Dyke, M W; Tainsky, M A

    1993-01-01

    AP-2 is a retinoic acid-inducible and developmentally regulated activator of transcription. We have cloned an alternative AP-2 transcript (AP-2B) from the human teratocarcinoma cell line PA-1, which encodes a protein differing in the C terminus from the previously isolated AP-2 protein (AP-2A). This protein contains the activation domain of AP-2 and part of the DNA binding domain but lacks the dimerization domain which is necessary for DNA binding. Analysis of overlapping genomic clones spann...

  6. Bending of protonema cells in a plastid glycolate/glycerate transporter knockout line of Physcomitrella patens.

    Directory of Open Access Journals (Sweden)

    Jin Nakahara

    Full Text Available Arabidopsis LrgB (synonym PLGG1 is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO. We isolated two homologous genes (PpLrgB1 and B2 from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB-GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2 and double (∆B1/∆B2-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.

  7. The Value of Information in Container Transport: Leveraging the Triple Bottom Line

    NARCIS (Netherlands)

    R.A. Zuidwijk (Rob); A.W. Veenstra (Albert)

    2010-01-01

    textabstractPlanning the transport of maritime containers from the sea port to final destinations while using multiple transport modes is challenged by uncertainties regarding the time the container is released for further transport or the transit time from the port to its final destination. This pa

  8. Contactless transport of matter in the first five resonance modes of a line-focused acoustic manipulator.

    Science.gov (United States)

    Foresti, Daniele; Nabavi, Majid; Poulikakos, Dimos

    2012-02-01

    The first five resonance modes for transport of matter in a line-focused acoustic levitation system are investigated. Contactless transport was achieved by varying the height between the radiating plate and the reflector. Transport and levitation of droplets in particular involve two limits of the acoustic forces. The lower limit corresponds to the minimum force required to overcome the gravitational force. The upper limit corresponds to the maximum acoustic pressure beyond which atomization of the droplet occurs. As the droplet size increases, the lower limit increases and the upper limit decreases. Therefore to have large droplets levitated, relatively flat radiation pressure amplitude during the translation is needed. In this study, using a finite element model, the Gor'kov potential was calculated for different heights between the reflector and the radiating plate. The application of the Gor'kov potential was extended to study the range of droplet sizes for which the droplets can be levitated and transported without atomization. It was found that the third resonant mode (H(3)-mode) represents the best compromise between high levitation force and smooth pattern transition, and water droplets of millimeter radius can be levitated and transported. The H(3)-mode also allows for three translation lines in parallel.

  9. Contactless transport of matter in the first five resonance modes of a line-focused acoustic manipulator.

    Science.gov (United States)

    Foresti, Daniele; Nabavi, Majid; Poulikakos, Dimos

    2012-02-01

    The first five resonance modes for transport of matter in a line-focused acoustic levitation system are investigated. Contactless transport was achieved by varying the height between the radiating plate and the reflector. Transport and levitation of droplets in particular involve two limits of the acoustic forces. The lower limit corresponds to the minimum force required to overcome the gravitational force. The upper limit corresponds to the maximum acoustic pressure beyond which atomization of the droplet occurs. As the droplet size increases, the lower limit increases and the upper limit decreases. Therefore to have large droplets levitated, relatively flat radiation pressure amplitude during the translation is needed. In this study, using a finite element model, the Gor'kov potential was calculated for different heights between the reflector and the radiating plate. The application of the Gor'kov potential was extended to study the range of droplet sizes for which the droplets can be levitated and transported without atomization. It was found that the third resonant mode (H(3)-mode) represents the best compromise between high levitation force and smooth pattern transition, and water droplets of millimeter radius can be levitated and transported. The H(3)-mode also allows for three translation lines in parallel. PMID:22352478

  10. Stress responses of juvenile matrinxã (Brycon amazonicus after transport in a closed system under different loading densities Respostas de estresse de matrinxã (Brycon amazonicus juvenil após transporte em sistema fechado sob diferentes densidades de carga

    Directory of Open Access Journals (Sweden)

    Janessa Sampaio de Abreu

    2008-08-01

    Full Text Available Optimum loading density for the transport of matrinxã juveniles was investigated in a closed system with plastic bags. Transport was conducted for 4 h with fish (23.5± 0.4g and 11.6 (0.08cm starved for 24h at loading densities of 83g L-1 (D1, 125g L-1 (D2, 168g L-1 (D3 and 206g L-1 (D4. Fish were sampled before transport (BT, after transport (AT and 24h AT. The water quality was monitored before capturing fish in depuration tanks, after transport in plastic bags and in recovery tanks. Water oxygen decreased to values below 4mg L-1 in D2, D3 and D4, temperature was around 32°C, pH 6.5-6.78, total ammonia 1.09-1.7mg L-1, un-ionized ammonia 3.58-9.33x10³mg L-1 and alkalinity 134-165mg CaCO3 L-1. Blood cortisol and glucose concentrations increased in fish of all densities AT and recovered the BT levels 24h after arrival. Osmolality did not change AT but increased 24h AT in fish of all densities whereas plasma chloride decreased inversely to the loading densities after the procedure. Hematocrit decreased 24h AT in fish of all densities but no differences were verified in the number of erythrocytes. No mortality was registered in any treatment during the next week after transport. Matrinxã demonstrated to be a crowding tolerant-species in transport operations besides tolerating low levels of oxygen in the water.Neste estudo, foram investigadas as densidades de carga adequadas para transporte de matrinxãs juvenis em sistema fechado com sacos plásticos. O transporte de 4h foi feito com peixes (23,5±0,4g; 11,6 (0,08cm em jejum por 24h, em densidades de 83g L-1 (D1, 125g L-1 (D2, 168g L-1 (D3 e 206g L-1 (D4. Os peixes foram amostrados antes do transporte (AT, logo após o transporte (chegada (DT e 24h depois. A qualidade da água foi monitorada antes da captura dos peixes nos tanques de depuração, após o transporte nos sacos plásticos e nos tanques de recuperação. O oxigênio da água diminuiu para valores inferiores a 4mg L-1 em D2, D3 e D4

  11. The switch interface and the interlock protection of the magnet power system of the beam current transport line

    International Nuclear Information System (INIS)

    The synchrotron radiation facility, constructed by the Hefei National Synchrotron Radiation Laboratory, University of Science and Technology of China, had passed the normal check by the state. The facility will be open to the users inside and outside the country. In this paper, the switch interface and the interlock protection of the magnet power system of the beam current transport line, one of the main components of this facility, are described

  12. Sea-level rise impacts on transport infrastructure: The notorious case of the coastal railway line at Dawlish, England

    OpenAIRE

    Dawson, DA; Shaw, J; Gehrels, WR

    2016-01-01

    Future climate change is likely to increase the frequency of coastal storms and floods, with major consequences for coastal transport infrastructure. This paper assesses the extent to which projected sea-level rise is likely to impact upon the functioning of the Dawlish to Teignmouth stretch of the London to Penzance railway line, in England. Using a semi-empirical modelling approach, we identify a relationship between sea-level change and rail incidents over the last 150 years and then use m...

  13. In-situ characterization of wastewater flow and transport from at-grade line sources to shallow groundwater

    Science.gov (United States)

    Weldeyohannes, A. O.; Kachanoski, R. G.; Dyck, M. F.

    2011-12-01

    A better understanding of multidimensional unsaturated and saturated flow and transport under boundary conditions typical of on-site wastewater disposal systems is required to assess the risk to groundwater contamination. The main objective of this research is to characterize in-situ wastewater flow and transport from at-grade line sources on a shallow groundwater conditions. The research site was conducted at Wetaskiwin Rest Stop, Alberta, Canada, where ultraviolet disinfected wastewater has been disposed off to the ground via pressurized at-grade line sources since 2007. The site was characterized for wastewater plume and temporal groundwater fluctuation by using Electromagnetic induction (EM31) and (EM38); and by grid of 74 water table wells, 14 piezometers and 11 transducers. Groundwater was analyzed for selected tracers (pH, EC and Cl) and some microbiology (e.g. E. coli). From the results wastewater plume was identified; and wastewater plume center of mass and average flow direction were estimated. Along the horizontal plume center of mass, 30 monitoring wells in 10 nests and 31 temperature sensors in 5 nests were installed to get vertical resolution of the wastewater plume and to track contaminant transport over time. Results, implications and plans for future investigations will be presented. The research output will benefit future research on contaminant fate and transport and groundwater risk assessment plans. Key words: On-site wastewater treatment/disposal system, Wastewater plume, Groundwater contamination.

  14. The APS ceramic chambers

    Energy Technology Data Exchange (ETDEWEB)

    Milton, S.; Warner, D.

    1994-07-01

    Ceramics chambers are used in the Advanced Photon Source (APS) machines at the locations of the pulsed kicker and bumper magnets. The ceramic will be coated internally with a resistive paste. The resistance is chosen to allow the low frequency pulsed magnet field to penetrate but not the high frequency components of the circulating beam. Another design goal was to keep the power density experienced by the resistive coating to a minimum. These ceramics, their associated hardware, the coating process, and our recent experiences with them are described.

  15. Influence of a hot and humid environment on thermal transport across the interface between a Ag thin-film line and a substrate

    Science.gov (United States)

    Li, Yuan; Noguchi, Kyohei; Saka, Masumi

    2016-04-01

    To evaluate the reliability of Ag thin-film lines for a wide range of applications in electronic devices, knowledge of the thermal transport across the interface between the line and the underlying substrate is of great importance. This is because such thermal transport significantly affects the temperature distribution in the line, the electrical performance of the line and the service life of the device the line is installed on. In this work, we examine the influence of a hot and humid environment on the thermal transport across the interface between a Ag thin-film line and a substrate. By performing a series of current-stressing experiments using the four-point probe method at atmospheric conditions (296 K and 30 RH%) on a Ag thin-film line for different durations of exposure to a hot and humid environment (323 K and 90 RH%), the electrical resistivity was found to increase with the exposure duration. Such an increase is believed to be the result of a decrease in the interfacial thermal conductance, which indicates less thermal transport from the line to the substrate. Moreover, by observing the surface morphology changes in the line and conducting a one-dimensional electro-thermal analysis, such variations can be attributed to the generation and growth of voids within the line, which hinder heat transfer from the line to the substrate through the interface.

  16. Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines.

    Science.gov (United States)

    Yanagida, O; Kanai, Y; Chairoungdua, A; Kim, D K; Segawa, H; Nii, T; Cha, S H; Matsuo, H; Fukushima, J; Fukasawa, Y; Tani, Y; Taketani, Y; Uchino, H; Kim, J Y; Inatomi, J; Okayasu, I; Miyamoto, K; Takeda, E; Goya, T; Endou, H

    2001-10-01

    System L is a major nutrient transport system responsible for the transport of large neutral amino acids including several essential amino acids. We previously identified a transporter (L-type amino acid transporter 1: LAT1) subserving system L in C6 rat glioma cells and demonstrated that LAT1 requires 4F2 heavy chain (4F2hc) for its functional expression. Since its oncofetal expression was suggested in the rat liver, it has been proposed that LAT1 plays a critical role in cell growth and proliferation. In the present study, we have examined the function of human LAT1 (hLAT1) and its expression in human tissues and tumor cell lines. When expressed in Xenopus oocytes with human 4F2hc (h4F2hc), hLAT1 transports large neutral amino acids with high affinity (K(m)= approximately 15- approximately 50 microM) and L-glutamine and L-asparagine with low affinity (K(m)= approximately 1.5- approximately 2 mM). hLAT1 also transports D-amino acids such as D-leucine and D-phenylalanine. In addition, we show that hLAT1 accepts an amino acid-related anti-cancer agent melphalan. When loaded intracellularly, L-leucine and L-glutamine but not L-alanine are effluxed by extracellular substrates, confirming that hLAT1 mediates an amino acid exchange. hLAT1 mRNA is highly expressed in the human fetal liver, bone marrow, placenta, testis and brain. We have found that, while all the tumor cell lines examined express hLAT1 messages, the expression of h4F2hc is varied particularly in leukemia cell lines. In Western blot analysis, hLAT1 and h4F2hc have been confirmed to be linked to each other via a disulfide bond in T24 human bladder carcinoma cells. Finally, in in vitro translation, we show that hLAT1 is not a glycosylated protein even though an N-glycosylation site has been predicted in its extracellular loop, consistent with the property of the classical 4F2 light chain. The properties of the hLAT1/h4F2hc complex would support the roles of this transporter in providing cells with essential

  17. PolyADP-ribose polymerase is a coactivator for AP-2-mediated transcriptional activation.

    OpenAIRE

    Kannan, P; Yu, Y; Wankhade, S; Tainsky, M A

    1999-01-01

    Overexpression of transcription factor AP-2 has been implicated in the tumorigenicity of the human teratocarcinoma cell lines PA-1 that contain an activated ras oncogene. Here we show evidence that overexpression of AP-2 sequesters transcriptional coactivators which results in self-inhibition. We identified AP-2-interacting proteins and determined whether these proteins were coactivators for AP-2-mediated transcription. One such interacting protein is polyADP-ribose polymerase (PARP). PARP su...

  18. CGM ApS Årsberetning til DANAK

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2003. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, ErhvervsfremmeStyrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler.......Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2003. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, ErhvervsfremmeStyrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler....

  19. Transepithelial transport of putrescine across monolayers of the human intestinal epithelial cell line, Caco-2

    Institute of Scientific and Technical Information of China (English)

    Vladan Milovic; Lyudmila Turchanowa; Jurgen Stein; Wolfgang F. Caspary

    2001-01-01

    AIM To study the transepithelial transport characteristics of the polyamine putrescine in human intestinal Caco-2 cell monolayers to elucidate the mechanisms of the putrescine intestinal absorption.METHODS The transepithelial transport and the cellular accumulation of putrescine was measured using Caco 2 cell monolayers grown on permeable filters.RESULTS Transepithelial transport of putrescine in physiological concentrations (>0.5 mM)from the apical to basolateral side was linear. Intracellular accumulation of putrescine was higher in confluent than in fully differentiated Caco-2 cells, but still negligible (less than 0.5%) of the overall transport across the monolayers in apical-to-basolateral direction. EGF enhanced putrescine accumulation in Caco-2 cells by four-fold, as well as putrescine conversion to spermidine and spermine by enhancing the activity of Sadenosylmethionine decarboxylase. However,EGF did not have any significant influence on putrescine flux across the Caco-2 cell monolayers. Excretion of putrescine from Caco-2cells into the basolateral medium did not exceed 50 picomoles, while putrescine passive flux from the apical to the basolateral chamber,contributed hundreds of micromoles polyamines to the basolateral chamber.CONCLUSION Transepithelial transport of putrescine across Caco-2 cell monolayers occurs in passive diffusion, and is not influenced when epithelial cells are stimulated to proliferate by a potent mitogen such as EGF.

  20. AP physics B crash course

    CERN Document Server

    Howell, Rebecca

    2012-01-01

    AP Physics B Crash Course - Get a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. Our AP Physics B Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know The Crash Course is based on an in-depth analysis of the AP Physics B course description outline and actual AP test questions. It covers only the information tested on the exam, so you can make the most of your valuable study time. Our easy-to-read format covers: mechanics, kinetic theory, t

  1. Organizing transportation on a double-track line under conditions of major overhaul on one track

    Directory of Open Access Journals (Sweden)

    Bošković Branislav

    2006-01-01

    Full Text Available The problem of traffic organization on a railway line during the execution of civil works on super and sub-structures maintenance under the present conditions is becoming more and more difficult to solve, above all, due to the increased traffic intensity. In order to make decisions on indispensable measures to be taken under such circumstances, one needs information on train compositions and their delays, line capacity, facilities, etc. In order to obtain such information, it is necessary to use a computer simulation which significantly enhances the range of the problem to be solved and offers answers to the questions that cannot be solved by analytical models alone. The paper presents a simulation model of the trains’ movement on a double-track line when one track is closed to traffic due to the work executions. Such model will provide a better quality of decision-making in the process of traffic organization under given circumstances.

  2. Evaluation of procedures to delimit services lines of urban transport areas with GIS

    Directory of Open Access Journals (Sweden)

    Antonio Moreno

    2003-01-01

    Full Text Available This paper addresses the evaluation of several procedures for delimiting service areas of urban transport facilities and computing the served population. To this end, detailed data of a local case study and a geographical information system are used. Adopting two different georeferencing methods and two spatial metrics, the tested approaches reveal disparate results and errors, which allow to appreciate the advantages and limitations of each one. It is expected that the conclusions will contribute to increase the accuracy of analysis focused to urban public transport planning and decision taking.

  3. Center for Geometrisk Metrologi, CGM ApS

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2002. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, Erhvervsfremme Styrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler (Teknisk Forskrift Nr. TF4 af 2000.11.0...

  4. Different Behavioral and Physiological Response in two Genetic Lines of Laying Hens Following Transportation

    Science.gov (United States)

    Physiological and behavioral response to transportation stress were examined in chickens selected for high group productivity and survivability (HGPS) resulting from reduced cannibalism and flightiness in colony cages and in chickens from Dekalb XL (DXL) commercial strain. At 13 wks of age, 96 pulle...

  5. Multidrug resistance mediated by ABC transporters in osteosarcoma cell lines: mRNA analysis and functional radiotracer studies

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, Celia Maria Freitas [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Faculty of Medicine, Institute of Biophysics/Biomathematics, IBILI, 3000-354 Coimbra (Portugal)]. E-mail: cgomes@ibili.uc.pt; van Paassen, Heidi [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Romeo, Salvatore [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Welling, Mick M. [Department of Radiology, Section of Nuclear Medicine, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Feitsma, R.I.J. [Department of Radiology, Section of Nuclear Medicine, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Abrunhosa, Antero J. [Faculty of Medicine, Institute of Biophysics/Biomathematics, IBILI, 3000-354 Coimbra (Portugal); Botelho, M. Filomena [Faculty of Medicine, Institute of Biophysics/Biomathematics, IBILI, 3000-354 Coimbra (Portugal); Hogendoorn, Pancras C.W. [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Pauwels, Ernest [Department of Radiology, Section of Nuclear Medicine, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Cleton-Jansen, Anne Marie [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands)

    2006-10-15

    Drug resistance remains a significant impediment to successful chemotherapy and constitutes a major prognostic factor in osteosarcoma (OS) patients. This study was designed to identify the role and prognostic significance of multidrug-resistance (MDR)-related transporters, such as multidrug resistance protein 1 (MDR1), multidrug-resistance-associated protein (MRP1) and breast-cancer-related protein (BCRP), in OS using cationic lipophilic radiotracers. We evaluated the chemosensitivity of four OS cell lines (Saos-2, 143B, MNNG/HOS and U-2OS) to doxorubicin (DOX), cisplatin (CIS) and methotrexate. The expression of MDR-related transporters was analyzed at mRNA level by quantitative polymerase chain reaction and at functional level by {sup 99m}Tc sestamibi and {sup 99m}Tc tetrofosmin. The effectiveness of MDR modulators [cyclosporin A (CsA) and imatinib] on transporter inhibition and on the reversal of resistance was also assessed. MNNG/HOS and U-2OS cells expressing high levels of MDR1 were highly resistant to DOX and showed reduced accumulation and higher efflux for radiotracers. Although MRP1 was uniformly expressed in all cells, only U-2OS was resistant to CIS. CsA restored sensitivity to DOX and CIS, and enhanced the accumulation and efflux half-life of radiotracers in MDR1-expressing cell lines. The chemosensitivity of OS cells to DOX was strongly dependent on mRNA MDR1 expression and could be circumvented by adding CsA. The kinetic parameters of radiotracers correlated with MDR1 expression levels, hence predicting DOX resistance. We concluded that sensitivity to chemotherapy is strongly dependent on the expression of MDR1 transporter and that radiotracer studies could prove clinically useful in predicting chemotherapy response and in evaluating the efficacy of MDR-reversing agents.

  6. Space charge compensation in the Linac4 low energy beam transport line with negative hydrogen ions

    Energy Technology Data Exchange (ETDEWEB)

    Valerio-Lizarraga, Cristhian A., E-mail: cristhian.alfonso.valerio.lizarraga@cern.ch [CERN, Geneva (Switzerland); Departamento de Investigación en Física, Universidad de Sonora, Hermosillo (Mexico); Lallement, Jean-Baptiste; Lettry, Jacques; Scrivens, Richard [CERN, Geneva (Switzerland); Leon-Monzon, Ildefonso [Facultad de Ciencias Fisico-Matematicas, Universidad Autónoma de Sinaloa, Culiacan (Mexico); Midttun, Øystein [CERN, Geneva (Switzerland); University of Oslo, Oslo (Norway)

    2014-02-15

    The space charge effect of low energy, unbunched ion beams can be compensated by the trapping of ions or electrons into the beam potential. This has been studied for the 45 keV negative hydrogen ion beam in the CERN Linac4 Low Energy Beam Transport using the package IBSimu [T. Kalvas et al., Rev. Sci. Instrum. 81, 02B703 (2010)], which allows the space charge calculation of the particle trajectories. The results of the beam simulations will be compared to emittance measurements of an H{sup −} beam at the CERN Linac4 3 MeV test stand, where the injection of hydrogen gas directly into the beam transport region has been used to modify the space charge compensation degree.

  7. AP-42 REVISION: COKE OVENS

    Science.gov (United States)

    The document "Compilation of Air Pollutant Emission Factors" (AP-42) has been published by the U. S. Environmental Protection Agency (EPA) since 1972. Supplements to AP-42 have been routinely published to add new emission source categories and to update existing emission factor...

  8. Appraisal of increased public transport capacity: the case of a new metro line to Nacka, Sweden

    OpenAIRE

    Cats , Oded; West , Jens; ELIASSON, Jonas

    2015-01-01

    One of the most common motivations for public transport investments is increased capacity. However, appraisal methodologies for projects meant to increase capacity are relatively less well developed compared to methodologies for projects aiming to reduce travel times. Each of the consequences of capacity limitations - crowding, risk for denied boarding and unreliable waiting and travel times - can increase the generalized travel costs. The appraisal of capacity improvements requires supply an...

  9. Going below the line: creating transportable brands for Australia's dark market

    Science.gov (United States)

    Carter, S

    2003-01-01

    Objective: To investigate non-point-of-sale cigarette marketing in Australia, one of the "darkest" markets in the world. Design: Analysis of 172 tobacco industry documents. Results: The tobacco industry has continued to market their products despite severe restrictions on legal marketing activity. They made careful plans to circumvent regulation well in advance. In preparation for bans, they chose and strengthened existing brands to enable their continued success in a dark market and prepared the consumer for bans by increasing their spending on below the line activities. Bans reduced the industry's effectiveness and efficiency. After bans new brand launches stopped: instead key existing brands were strengthened via alterations to the product, line extensions, and stretching loopholes in the legislation as far as possible. In line with the general trend towards integrated marketing, a range of activities have been used in combination, including guerrilla marketing, advertising in imported international magazines, altering the pack, sponsorships, brand stretching, event promotions, lifestyle premiums, and the development of corporate websites. Conclusions: The tobacco industry acknowledges that marketing restrictions have an impact, validating their continued use in tobacco control. The industry is extremely creative in circumventing these marketing restrictions, requiring tobacco marketing regulations to be informed by marketing expertise, regularly updated, and to adopt the broadest possible scope. Tobacco control advocates, particularly those communicating with young people, could learn from the creativity of the tobacco industry. PMID:14645953

  10. Integral Assessment Of Inactive Rail Lines Jsc “Russian Railways” As The Basis For Effective And Reasonable Use Of Their Infrastructure In The Regional Transport Network

    Directory of Open Access Journals (Sweden)

    Nina Fridrikhovna Sirina

    2015-03-01

    Full Text Available In the present paper, we consider the organizational and economic mechanism of relations between the participants of the transportation process for the efficient use of infrastructure of inactive rail lines JSC “ Russian Railways “ (the lines. The purpose of the work is to develop tools to support the cost-effective use of infrastructure lines. One of the productive directions in solving the problem of inefficient use of infrastructure lines in modern conditions of the restructuring of “Russian Railways” is an integral criterion of the lines based on the theory of active systems. Using a model of relations between members of the transportation process will enable to ground the expediency of the use of infrastructure lines and consolidate joint efforts in the region, businesses, and JSC “Russian Railways” for the goal.

  11. Final report for tank 241-AP-101, grab samples 1AP-95-1, 1AP-95-2, 1AP-95-3, 1AP-95-4, 1AP-95-5, and 1AP-95-6

    Energy Technology Data Exchange (ETDEWEB)

    Esch, R.A.

    1996-03-04

    Six supernate grab samples (1AP-95-1 through 6) and one field blank (1AP-95-7) were taken from tank 241-AP-101, on Nov. 10 and 13, 1995. Analyses were performed in support of the Safety Screening and the Waste Compatibility Safety programs. All analytical results were within the action limits stated in the TSAP.

  12. The Dopamine Transporter Constitutively Internalizes and Recycles in a Protein Kinase C-regulated Manner in Stably Transfected PC12 Cell Lines*

    OpenAIRE

    Loder, Merewyn K; Melikian, Haley E.

    2003-01-01

    The dopamine transporter (DAT) removes dopamine from the extracellular milieu and is potently inhibited by number of psychoactive drugs, including cocaine, amphetamines, and methylphenidate (Ritalin). Multiple lines of evidence demonstrate that protein kinase C (PKC) down-regulates dopamine transport, primarily by redistributing DAT from the plasma membrane to endosomal compartments, although the mechanisms facilitating transporter sequestration are not defined. Here, we demonstrate that DAT ...

  13. High intensity proton beam transportation through fringe field of 70 MeV compact cyclotron to beam line targets

    Science.gov (United States)

    Zhang, Xu; Li, Ming; Wei, Sumin; Xing, Jiansheng; Hu, Yueming; Johnson, Richard R.; Piazza, Leandro; Ryjkov, Vladimir

    2016-06-01

    From the stripping points, the high intensity proton beam of a compact cyclotron travels through the fringe field area of the machine to the combination magnet. Starting from there the beams with various energy is transferred to the switching magnet for distribution to the beam line targets. In the design of the extraction and transport system for the compact proton cyclotron facilities, such as the 70 MeV in France and the 100 MeV in China, the space charge effect as the beam crosses the fringe field has not been previously considered; neither has the impact on transverse beam envelope coupled from the longitudinal direction. Those have been concerned much more with the higher beam-power because of the beam loss problem. In this paper, based on the mapping data of 70 MeV cyclotron including the fringe field by BEST Cyclotron Inc (BEST) and combination magnet field by China Institute of Atomic Energy (CIAE), the beam extraction and transport are investigated for the 70 MeV cyclotron used on the SPES project at Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Legnaro (INFN-LNL). The study includes the space charge effect and longitudinal and transverse coupling mentioned above, as well as the matching of beam optics using the beam line for medical isotope production as an example. In addition, the designs of the ±45° switching magnets and the 60° bending magnet for the extracted beam with the energy from 35 MeV to 70 MeV have been made. Parts of the construction and field measurements of those magnets have been done as well. The current result shows that, the design considers the complexity of the compact cyclotron extraction area and fits the requirements of the extraction and transport for high intensity proton beam, especially at mA intensity levels.

  14. Space Charge Compensation in the Linac4 Low Energy Beam Transport Line with Negative Hydrogen Ions

    CERN Document Server

    Valerio-Lizarraga, C; Leon-Monzon, I; Lettry, J; Midttun, O; Scrivens, R

    2014-01-01

    The space charge effect of low energy, unbunched ion beams can be compensated by the trapping of ions or electrons into the beam potential. This has been studied for the 45 keV negative hydrogen ion beam in the CERN Linac4 Low Energy Beam Tranport (LEBT) using the package IBSimu1, which allows the space charge calculation of the particle trajectories. The results of the beam simulations will be compared to emittance measurements of an H- beam at the CERN Linac4 3 MeV test stand, where the injection of hydrogen gas directly into the beam transport region has been used to modify the space charge compensation degree.

  15. Quantitative Kα line spectroscopy for energy transport in ultra-intense laser plasma interaction

    Science.gov (United States)

    Zhang, Z.; Nishimura, H.; Fujioka, S.; Arikawa, Y.; Nakai, M.; Chen, H.; Park, J.; Williams, G. J.; Ozaki, T.; Shiraga, H.; Kojima, S.; Johzaki, T.; Sunahara, A.; Miyanaga, N.; Kawanaka, J.; Nakata, Y.; Jitsuno, T.; Azechi, H.

    2016-03-01

    Absolute Ka line spectroscopy is proposed for studying laser-plasma interactions taking place in the cone-guided fast ignition targets. X-ray spectra ranging from 20 to 100 keV were quantitatively measured with a Laue spectrometer. The absolute sensitivities of the Laue spectrometer system were calibrated using pre-characterized laser-produced x-ray sources and radioisotopes. The integrated reflectivity for the crystal is in good agreement with predictions by an open code for x-ray diffraction. The energy transfer efficiency from incident laser beams to hot electrons, as the energy transfer agency, is derived as a consequence of this work. The absolute yield of Au and Ta Ka lines were measured in the fast ignition experimental campaign performed at Institute of Laser Engineering, Osaka University. Applying the hot electron spectrum information from the electron spectrometer, an energy transfer efficiency of the incident LFEX [1], a kJ-class PW laser, to hot electrons was derived for a planar and cone-guided geometry.

  16. SN 2005ap: A Most Brilliant Explosion

    CERN Document Server

    Quimby, Robert M; Wheeler, J Craig; Höflich, Peter; Akerlof, Carl W; Rykoff, Eli S

    2007-01-01

    We present unfiltered photometric observations with ROTSE-III and optical spectroscopic follow-up with the HET and Keck of the most luminous supernova yet identified, SN 2005ap. The spectra taken about 3 days before and 6 days after maximum light show narrow emission lines (likely originating in the dwarf host) and absorption lines at a redshift of z=0.2832, which puts the peak unfiltered magnitude at -22.7 +/- 0.1 absolute. Broad P-Cygni features corresponding to H-alpha, CIII, NIII, and OIII, are further detected with a photospheric velocity of ~20,000 km/s. Unlike other highly luminous supernovae such as 2006gy and 2006tf that show slow photometric evolution, the light curve of SN 2005ap indicates a 1-3 week rise to peak followed by a relatively rapid decay. The spectra also lack the distinct emission peaks from moderately broadened (FWHM ~ 2,000 km/s) Balmer lines seen in SN 2006gy and SN 2006tf. We briefly discuss the origin of the extraordinary luminosity from a strong interaction as may be expected fro...

  17. Study of beam transport lines for a biomedical research facility at CERN based on LEIR

    CERN Document Server

    Abler, D; Garonna, A; Peach, K

    2014-01-01

    The Low Energy Ion Ring (LEIR) at CERN has been proposed to provide ion beams with magnetic rigidities up to 6.7 T.m for biomedical research, in parallel to its continued operation for LHC and SPS fixed target physics experiments. In the context of this project, two beamlines are proposed for transporting the extracted beam to future experimental end-stations: a vertical beamline for specific low-energy radiobiological research, and a horizontal beamline for radiobiology and medical physics experimentation. This study presents a first linear-optics design for the delivery of 1–5mm FWHM pencil beams and 5 cm 5 cm homogeneous broad beams to both endstations. High field uniformity is achieved by selection of the central part of a strongly defocused Gaussian beam, resulting in low beam utilisation.

  18. Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.

    Directory of Open Access Journals (Sweden)

    Tiago dos Santos

    Full Text Available Nanotechnology is expected to play a vital role in the rapidly developing field of nanomedicine, creating innovative solutions and therapies for currently untreatable diseases, and providing new tools for various biomedical applications, such as drug delivery and gene therapy. In order to optimize the efficacy of nanoparticle (NP delivery to cells, it is necessary to understand the mechanisms by which NPs are internalized by cells, as this will likely determine their ultimate sub-cellular fate and localisation. Here we have used pharmacological inhibitors of some of the major endocytic pathways to investigate nanoparticle uptake mechanisms in a range of representative human cell lines, including HeLa (cervical cancer, A549 (lung carcinoma and 1321N1 (brain astrocytoma. Chlorpromazine and genistein were used to inhibit clathrin and caveolin mediated endocytosis, respectively. Cytochalasin A and nocodazole were used to inhibit, respectively, the polymerisation of actin and microtubule cytoskeleton. Uptake experiments were performed systematically across the different cell lines, using carboxylated polystyrene NPs of 40 nm and 200 nm diameters, as model NPs of sizes comparable to typical endocytic cargoes. The results clearly indicated that, in all cases and cell types, NPs entered cells via active energy dependent processes. NP uptake in HeLa and 1321N1 cells was strongly affected by actin depolymerisation, while A549 cells showed a stronger inhibition of NP uptake (in comparison to the other cell types after microtubule disruption and treatment with genistein. A strong reduction of NP uptake was observed after chlorpromazine treatment only in the case of 1321N1 cells. These outcomes suggested that the same NP might exploit different uptake mechanisms to enter different cell types.

  19. The GEANT4 toolkit capability in the hadron therapy field: simulation of a transport beam line

    Science.gov (United States)

    Cirrone, G. A. P.; Cuttone, G.; Di Rosa, F.; Raffaele, L.; Russo, G.; Guatelli, S.; Pia, M. G.

    2006-01-01

    At Laboratori Nazionali del Sud of the Instituto Nazionale di Fisica Nucleare of Catania (Sicily, Italy), the first Italian hadron therapy facility named CATANA (Centro di AdroTerapia ed Applicazioni Nucleari Avanzate) has been realized. Inside CATANA 62 MeV proton beams, accelerated by a superconducting cyclotron, are used for the radiotherapeutic treatments of some types of ocular tumours. Therapy with hadron beams still represents a pioneer technique, and only a few centers worldwide can provide this advanced specialized cancer treatment. On the basis of the experience so far gained, and considering the future hadron-therapy facilities to be developed (Rinecker, Munich Germany, Heidelberg/GSI, Darmstadt, Germany, PSI Villigen, Switzerland, CNAO, Pavia, Italy, Centro di Adroterapia, Catania, Italy) we decided to develop a Monte Carlo application based on the GEANT4 toolkit, for the design, the realization and the optimization of a proton-therapy beam line. Another feature of our project is to provide a general tool able to study the interactions of hadrons with the human tissue and to test the analytical-based treatment planning systems actually used in the routine practice. All the typical elements of a hadron-therapy line, such as diffusers, range shifters, collimators and detectors were modelled. In particular, we simulated the Markus type ionization chamber and a Gaf Chromic film as dosimeters to reconstruct the depth (Bragg peak and Spread Out Bragg Peak) and lateral dose distributions, respectively. We validated our simulated detectors comparing the results with the experimental data available in our facility.

  20. The West African Squall Line Observed on 23 June 1981 during COPT 81: Mesoscale Structure and Transports.

    Science.gov (United States)

    Chalon, J. P.; Jaubert, G.; Lafore, J. P.; Roux, F.

    1988-10-01

    Durirg the night of 23/24 June 1981, new Korhogo, Ivory Coast, a squall line passed over the instrumented area of the COPT 81 experiment. Observations were obtained with a dual-Doppler radar system, a sounding station and 22 automatic meteorological surface stations. Data from these instruments and from satellite pictures were analyzed to depict the kinematic and thermodynamic structure of the squall line. Composite analysis techniques were used to obtain a vertical cross section of the reflectivity structure and of the wind field relative to the line. The redistributions of air, moisture and thermodynamic energy by the convection wet calculated through averaged two-dimensional wind fields from a dual-Doppler radar system. The method also allowed the evaluation of the exchanges that were occurring between the convective and the stratiform regions.This squall line had many similarities with tropical squall lines previously described by others. The leading convective part, composed of intense updrafts and downdrafts, and the trailing part, containing weak mesoscale updraft and downdraft, were separated by a reflectivity trough. A notable feature of this line was the presence of a leading anvil induced by intense easterly environmental winds in the upper troposphere. Observations of the evolution of the system at different scales indicated that the mesoalpha-scale (following the classification of Orlanski) and the mosobeta-scale patterns combined to allow the system to have optimum conditions for maximum strength and a maximum lifetime.A rear-to-front flow was found at midlevels in the stratiform region. The flow sloped downward to the surface and took on the characteristics of a density current in the forward half of the squall lice. Entering the convective region, this flow was supplied with cold air by the convective downdrafts and played an important role in forcing upward the less dense monsoon flow entering at the leading edge.Calculations of mass, moisture and

  1. Meet the APS Journal Editors

    Science.gov (United States)

    2016-05-01

    The Editors of the APS journals invite you to join them for conversation. The Editors will be available to answer questions, hear your ideas, and discuss any comments about the journals. All are welcome. Light refreshments will be served.

  2. A transverse emittance and acceptance measurement system in a low-energy beam transport line

    Energy Technology Data Exchange (ETDEWEB)

    Kashiwagi, H., E-mail: kashiwagi.hirotsugu@jaea.go.jp; Miyawaki, N.; Kurashima, S.; Okumura, S. [Department of Advanced Radiation Technology, Japan Atomic Energy Agency, 1233 Watanuki, Takasaki, Gunma 370-1292 (Japan)

    2014-02-15

    A transverse beam emittance and acceptance measurement system has been developed to visualize the relationship between the injected beam emittance and the acceptance of a cyclotron. The system is composed of a steering magnet, two pairs of slits to limit the horizontal and vertical phase-space, a beam intensity detector just behind the slits for the emittance measurement, and a beam intensity detector in the cyclotron for the acceptance measurement. The emittance is obtained by scanning the slits and measuring the beam intensity distribution. The acceptance is obtained by measuring the distribution of relative beam transmission by injecting small emittance beams at various positions in a transverse phase-space using the slits. In the acceptance measurement, the beam from an ion source is deflected to the defined region by the slits using the steering magnet so that measurable acceptance area covers a region outside the injection beam emittance. Measurement tests were carried out under the condition of accelerating a beam of {sup 16}O{sup 6+} from 50.2 keV to 160 MeV. The emittance of the injected beam and the acceptance for accelerating and transporting the beam to the entrance of the extraction deflector were successfully measured. The relationship between the emittance and acceptance is visualized by displaying the results in the same phase-plane.

  3. Epidermal growth factor inhibits glycylsarcosine transport and hPepT1 expression in a human intestinal cell line

    DEFF Research Database (Denmark)

    Nielsen, C U; Amstrup, J; Steffansen, B;

    2001-01-01

    The human intestinal cell line Caco-2 was used as a model system to study the effects of epidermal growth factor (EGF) on peptide transport. EGF decreased apical-to-basolateral fluxes of [(14)C]glycylsarcosine ([(14)C]Gly-Sar) up to 50.2 +/- 3.6% (n = 6) of control values. Kinetic analysis...... of the fluxes showed that maximal flux (V(max)) of transepithelial transport decreased from 3.00 +/- 0.17 nmol x cm(-2) x min(-1) in control cells to 0.50 +/- 0.07 nmol x cm(-2) x min(-1) in cells treated with 5 ng/ml EGF (n = 6, P ... = 6) in control cells and 1.89 +/- 0.28 mM (n = 6, not significantly different from control) in EGF-treated cells. Similarly, apical uptake of [(14)C]Gly-Sar decreased in cells treated with EGF, with an ED(50) value of 0.36 +/- 0.06 ng/ml (n = 6) EGF and a maximal inhibition of 80 +/- 0.02% (n = 6). V...

  4. Railroad Lines - RAIL_SYSTEM_ACTIVE_ABANDONED_INDOT_IN: Active and Abandoned Rail System in Indiana, 2005 (Indiana Department of Transportation, 1:1200, Line Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — RAIL_SYSTEM_ACTIVE_ABANDONED_INDOT_IN is a line shapefile that contains all active and abandoned rail lines in Indiana, provided by personnel of Indiana Department...

  5. Transcription factor AP-2gamma is a developmentally regulated marker of testicular carcinoma in situ and germ cell tumors

    DEFF Research Database (Denmark)

    Hoei-Hansen, Christina E; Nielsen, John E; Almstrup, Kristian;

    2004-01-01

    and protein level in normal human tissues and a panel of tumors and tumor-derived cell lines. In the gonads, we established the ontogeny of expression of AP-2gamma in normal and dysgenetic samples. We also investigated the regulation of AP-2gamma by steroids and retinoic acid. RESULTS: We detected abundant AP......-2gamma in testicular CIS and in testicular germ cell tumors of young adults and confirmed differential expression of AP-2gamma in somatic tumors. We found that AP-2gamma expression was regulated by retinoic acid in an embryonal carcinoma cell line (NT2). The investigation of ontogeny of AP-2gamma....... CONCLUSIONS: AP-2gamma is developmentally regulated and associated with the undifferentiated phenotype in germ cells. This transcription factor may be involved in self-renewal and survival of immature germ cells and tissue-specific stem cells. AP-2gamma is a novel marker of testicular CIS and CIS...

  6. 一种新的小檗碱衍生物CPU-86017在人小肠上皮细胞(Caco-2细胞)中的转运与摄取特性%Transport and uptake characteristics of a new derivative of berberine (CPU-86017) by human intestinal epithelial cell line: Caco-2

    Institute of Scientific and Technical Information of China (English)

    杨海涛; 王广基

    2003-01-01

    AIM: The characteristics of transepithelial transport and uptake of CPU-86017 {[7-(4-chlorbenzyl)-7,8,13,13α tetrahydroberberine chloride, CTHB] }, a new antiarrhythmia agent and a new derivative of berberine, were investi gated on epithelial cell line (Caco-2) to further understand the absorption mechanism of berberine and its derivatives. METHODS: Caco-2 cell was used. RESULTS: 1) The permeability coefficient from the apical (AP) to basolateral (BL) of CPU-86017 was approximately 5 times higher than that from BL-to-AP transport. The effects of a P-glycoprotein (P-gp) inhibitor-cyclosporin A, some surfactants, and lower pH on the transepithelial transport of CPU-86017 were also observed. Cyclosporine A at 7.5 mg/L had no effect on the transepithelial electrical resistance (TEER); an about 4-fold enhancement on the transepithlial transport of CPU-86017 was observed. Some surfac tants (sodium citrate, sodium deoxycholate, and sodium dodecyl sulfate) at 100 μmol/L and low pH (pH=6.0) induced a reversible decrease of TEER; enhancements of the transepithelial transport of CPU-86017 were also observed with some surfactants; 2) In the process of uptake of CPU-86017, the initial uptake rates of CPU-86017 were saturable with a Vmax of (250+39) μg.min-1.g-1 (protein) and Km of (0.90+0.12) mmol/L. This process was enhanced by cyclosporine A (7.5 mg/L) with a Vmax of (588+49) μg.min-1.g-1 (protein) and Km (0.42+0.08) mmol/L. CONCLUSION: Some surfactants and P-gp inhibitors can be considered as enhancers of its transepithelial trans port and uptake.

  7. Epidermal growth factor decreases PEPT2 transport capacity and expression in the rat kidney proximal tubule cell line SKPT0193 cl.2

    DEFF Research Database (Denmark)

    Bravo, Silvina A; Nielsen, Carsten Uhd; Amstrup, Jan;

    2004-01-01

    transport capacity and expression in the rat proximal tubule cell line SKPT0193 cl.2 (SKPT), which expresses rat PEPT2 (rPEPT2) in the apical membrane. Treatment of SKPT cells with EGF during cell culture growth caused a dose-dependent decrease in rPEPT2 transport capacity and expression, as determined...... suggests that this might be disadvantageous when studying PEPT2-mediated transport phenomena. These findings demonstrate for the first time EGF-mediated regulation of PEPT2 expression in a kidney cell line. The relevance for kidney regulation of peptide transport activity in physiological and...... by studies of apical uptake of [14C]glycylsarcosine, rPepT2 mRNA levels, and immunostaining of SKPT cells with a rPEPT2-specific antibody. On the contrary, apical uptake of glucose and lysine was increased in EGF-treated cells, indicating that EGF was not acting generally to decrease apical nutrient uptake...

  8. Expression, localization, and inducibility by bile acids of hepatobiliary transporters in the new polarized rat hepatic cell lines, Can 3-1 and Can 10.

    OpenAIRE

    Cassio, Doris; Macias, Rocio,; Grosse, Brigitte; Marin, Jose,; Monte, Maria,

    2007-01-01

    International audience Sinusoidal and apical transporters are responsible for the uptake and biliary elimination of many compounds by hepatocytes. Few in vitro models are however available for analyzing such functions. The expression and bile-acid inducibility of 13 transporters and two nuclear receptors were investigated in the new rat polarized lines, Can 3-1 and Can 10, and in their unpolarized parent, Fao. The relative abundance of mRNA, the protein level, and their localization were e...

  9. APS Education and Diversity Efforts

    Science.gov (United States)

    Prestridge, Katherine; Hodapp, Theodore

    2015-11-01

    American Physical Society (APS) has a wide range of education and diversity programs and activities, including programs that improve physics education, increase diversity, provide outreach to the public, and impact public policy. We present the latest programs spearheaded by the Committee on the Status of Women in Physics (CSWP), with highlights from other diversity and education efforts. The CSWP is working to increase the fraction of women in physics, understand and implement solutions for gender-specific issues, enhance professional development opportunities for women in physics, and remedy issues that impact gender inequality in physics. The Conferences for Undergraduate Women in Physics, Professional Skills Development Workshops, and our new Professional Skills program for students and postdocs are all working towards meeting these goals. The CSWP also has site visit and conversation visit programs, where department chairs request that the APS assess the climate for women in their departments or facilitate climate discussions. APS also has two significant programs to increase participation by underrepresented minorities (URM). The newest program, the APS National Mentoring Community, is working to provide mentoring to URM undergraduates, and the APS Bridge Program is an established effort that is dramatically increasing the number of URM PhDs in physics.

  10. Overview of charged-particle beam diagnostics for the advanced photon source (APS)

    Science.gov (United States)

    Lumpkin, A. H.; Decker, G.; Kahana, E.; Patterson, D.; Sellyey, W.; Votaw, A.; Wang, X.; Chung, Y.

    1992-07-01

    Plans, prototypes, and initial test results for the charged-particle beam (e-,e+) diagnostic systems on the injector rings, their transport lines, and the storage ring for the Advanced Photon Source (APS) are presented. The APS will be a synchrotron radiation user facility with one of the world's brightest x-ray sources in the 10-keV to 100-keV regime. Its 200-MeV electron linac, 450-MeV positron linac, positron accumulator ring, 7-GeV booster synchrotron, 7-GeV storage ring, and undulator test lines will also demand the development and demonstration of key particle-beam characterization techniques over a wide range of parameter space. Some of these parameter values overlap or approach those projected for fourth generation light sources (linac-driven FELs and high brightness storage rings) as described at a recent workshop. Initial results from the diagnostics prototypes on the linac test stand operating at 45-MeV include current monitor data, beam loss monitor data, and video digitization using VME architecture.

  11. Charged-particle beam diagnostics for the Advanced Photon Source (APS)

    Science.gov (United States)

    Lumpkin, A. H.; Decker, G.; Kahana, E.; Patterson, D.; Sellyey, W.; Wang, X.; Chung, Y.

    1992-08-01

    Plans, prototypes, and initial test results for the charged-particle beam (e-), e(+) diagnostic systems on the injector rings, their transport lines, and the storage ring for the Advanced Photon Source (APS) are presented. The APS will be a synchrotron radiation user facility with one of the world's brightest x-ray sources in the 10-keV to 100-keV regime. Its 200-MeV electron linac, 450-MeV positron linac, positron accumulator ring, 7-GeV booster synchrotron, 7-GeV storage ring, and undulator test lines will also demand the development and demonstration of key particle-beam characterization techniques over a wide range of parameter space. Some of these parameter values overlap or approach those projected for fourth generation light sources (linac-driven FELs and high brightness storage rings) as described at a recent workshop. Initial results from the diagnostics prototypes on the linac test stand operating at 45-MeV include current monitor data, beam loss monitor data, and video digitization using VME architecture.

  12. Epidermal growth factor inhibits glycyl sarcosine transport and hPepT1 expression in a human intestinal cell line

    DEFF Research Database (Denmark)

    Nielsen, Carsten Uhd; Amstrup, Jan; Steffansen, Bente;

    2001-01-01

    Intestinal oligopeptide transporter, growth factor, immunocytochemistry, laser scanning confocal microscopy......Intestinal oligopeptide transporter, growth factor, immunocytochemistry, laser scanning confocal microscopy...

  13. TRANSFECTED MDCK CELL LINE WITH ENHANCED EXPRESSION OF CYP3A4 AND P-GLYCOPROTEIN AS A MODEL TO STUDY THEIR ROLE IN DRUG TRANSPORT AND METABOLISM

    OpenAIRE

    Kwatra, Deep; Budda, Balasubramanyam; Vadlapudi, Aswani Dutt; Vadlapatla, Ramya Krishna; Pal, Dhananjay; Ashim K. Mitra

    2012-01-01

    The aim of this study was to characterize and utilize MDCK cell line expressing CYP3A4 and P-glycoprotein as an in vitro model for evaluating drug-herb and drug-drugs of abuse interactions. MDCK cell line simultaneously expressing P-gp and CYP3A4 (MMC) was developed and characterized by using expression and activity studies. Cellular transport study of 200 μM cortisol was performed to determine their combined activity. The study was carried across MDCK-WT, MDCK-MDR1 and MMC cell lines. Simila...

  14. AP Geography, Environmental Science Thrive

    Science.gov (United States)

    Robelen, Erik W.

    2012-01-01

    Geography may not be particularly known as a hot topic among today's students--even some advocates suggest it suffers from an image problem--but by at least one measure, the subject is starting to come into its own. Across more than 30 topics covered in the Advanced Placement (AP) program, participation in geography is rising faster than any…

  15. Design of a high-current low-energy beam transport line for an intense D-T/D-D neutron generator

    Science.gov (United States)

    Lu, Xiaolong; Wang, Junrun; Zhang, Yu; Li, Jianyi; Xia, Li; Zhang, Jie; Ding, Yanyan; Jiang, Bing; Huang, Zhiwu; Ma, Zhanwen; Wei, Zheng; Qian, Xiangping; Xu, Dapeng; Lan, Changlin; Yao, Zeen

    2016-03-01

    An intense D-T/D-D neutron generator is currently being developed at the Lanzhou University. The Cockcroft-Walton accelerator, as a part of the neutron generator, will be used to accelerate and transport the high-current low-energy beam from the duoplasmatron ion source to the rotating target. The design of a high-current low-energy beam transport (LEBT) line and the dynamics simulations of the mixed beam were carried out using the TRACK code. The results illustrate that the designed beam line facilitates smooth transportation of a deuteron beam of 40 mA, and the number of undesired ions can be reduced effectively using two apertures.

  16. Design of medium energy beam transport line between the RFQ and the Linac in the radioactive ion beam facility at VECC, Kolkata

    Indian Academy of Sciences (India)

    S Dechoudhury; Vaishali Naik; Manas Mondal; Hemendra Kumar Pandey; Avik Chatterjee; Dirtha Sanyal; Debasis Bhowmick; Alok Chakrabarti

    2010-09-01

    The design of a medium energy beam transport (MEBT) line comprising of a re-buncher and four quadrupoles, two upstream and the other two downstream of the re-buncher, has been presented. The design was done to ensure almost 100% transport of heavy-ion beams of about 99 keV/u energy from RFQ having a / not less than 1/14 through the re-buncher and then through IH Linac of about 0.6 m length in which beam would be accelerated to about 185 keV/u. The re-buncher has been designed to operate at 37.8 MHz, the resonating frequency of both the RFQ and the IH Linac. The entire beam line has been installed and recently O5+ beam from RFQ has been transported through the re-buncher and subsequently accelerated in the IH Linac successfully.

  17. APS beamline standard components handbook

    International Nuclear Information System (INIS)

    It is clear that most Advanced Photon Source (APS) Collaborative Access Team (CAT) members would like to concentrate on designing specialized equipment related to their scientific programs rather than on routine or standard beamline components. Thus, an effort is in progress at the APS to identify standard and modular components of APS beamlines. Identifying standard components is a nontrivial task because these components should support diverse beamline objectives. To assist with this effort, the APS has obtained advice and help from a Beamline Standardization and Modularization Committee consisting of experts in beamline design, construction, and operation. The staff of the Experimental Facilities Division identified various components thought to be standard items for beamlines, regardless of the specific scientific objective of a particular beamline. A generic beamline layout formed the basis for this identification. This layout is based on a double-crystal monochromator as the first optical element, with the possibility of other elements to follow. Pre-engineering designs were then made of the identified standard components. The Beamline Standardization and Modularization Committee has reviewed these designs and provided very useful input regarding the specifications of these components. We realize that there will be other configurations that may require special or modified components. This Handbook in its current version (1.1) contains descriptions, specifications, and pre-engineering design drawings of these standard components. In the future, the APS plans to add engineering drawings of identified standard beamline components. Use of standard components should result in major cost reductions for CATs in the areas of beamline design and construction

  18. Radiation effects on active pixel sensors (APS)

    International Nuclear Information System (INIS)

    Active pixel sensor (APS) is a new generation of image sensors which presents several advantages relatively to charge coupled devices (CCDs) particularly for space applications (APS requires only 1 voltage to operate which reduces considerably current consumption). Irradiation was performed using 60Co gamma radiation at room temperature and at a dose rate of 150 Gy(Si)/h. 2 types of APS have been tested: photodiode-APS and photoMOS-APS. The results show that photoMOS-APS is more sensitive to radiation effects than photodiode-APS. Important parameters of image sensors like dark currents increase sharply with dose levels. Nevertheless photodiode-APS sensitivity is one hundred time lower than photoMOS-APS sensitivity

  19. The AP-2 family of transcription factors

    OpenAIRE

    Eckert, Dawid; Buhl, Sandra; Weber, Susanne; Jäger, Richard; Schorle, Hubert

    2005-01-01

    The AP-2 family of transcription factors consists of five different proteins in humans and mice: AP-2α, AP-2β, AP-2γ, AP-2δ and AP-2ε. Frogs and fish have known orthologs of some but not all of these proteins, and homologs of the family are also found in protochordates, insects and nematodes. The proteins have a characteristic helix-span-helix motif at the carboxyl terminus, which, together with a central basic region, mediates dimerization and DNA binding. The amino terminus contains the tra...

  20. Learning through delivery, Westinghouse AP1000 plant construction

    International Nuclear Information System (INIS)

    The AP1000 plant, which is a 1100 MWe class pressurized water reactor with passive safety features, is designed around a conventional 2 loop, 2 steam generator primary system configuration with 2 hot legs, 4 reactor coolant pumps directly mounted in the steam generator lower head and 4 cold legs. A particular feature of AP1000 is its modular construction to minimize the time and cost of construction. Modular construction allows activities to be run in parallel, it allows more activities to be performed in a controlled factory instead of in the field, and it provides a better level of quality. The AP1000 plant design includes 106 structural modules and 52 mechanical modules. Structural modules include all penetrations for piping, cable trays, HVAC duct runs, and all reinforcement for pipe, equipment hangers, and supports. Structural modules are shipped in sub-modules to support transportation by rail or truck or barge. Mechanical modules contain equipment such as pumps, tanks, heat exchangers, air-handling units, and filters along with interconnecting pipes, valves, instruments, wiring and support services. Modular construction requires strong coordination between engineering, supply chain and construction. A total of 8 AP1000 units are currently under construction in China and in the United States. The lessons learned and best practices of each new AP1000 construction are systematically incorporated into the standard design. (A.C.)

  1. Differential recognition of a dileucine-based sorting signal by AP-1 and AP-3 reveals a requirement for both BLOC-1 and AP-3 in delivery of OCA2 to melanosomes

    OpenAIRE

    Sitaram, Anand; Dennis, Megan K.; Chaudhuri, Rittik; De Jesus-Rojas, Wilfredo; Tenza, Danièle; Setty, Subba Rao Gangi; Wood, Christopher S.; Sviderskaya, Elena V.; Bennett, Dorothy C.; Raposo, Graça; Bonifacino, Juan S.; Marks, Michael S.

    2012-01-01

    Cell types that generate unique lysosome-related organelles (LROs), such as melanosomes in melanocytes, populate nascent LROs with cargoes that are diverted from endosomes. Cargo sorting toward melanosomes correlates with binding via cytoplasmically exposed sorting signals to either heterotetrameric adaptor AP-1 or AP-3. Some cargoes bind both adaptors, but the relative contribution of each adaptor to cargo recognition and their functional interactions with other effectors during transport to...

  2. The Role of the Clathrin Adaptor AP-1: Polarized Sorting and Beyond

    Directory of Open Access Journals (Sweden)

    Fubito Nakatsu

    2014-11-01

    Full Text Available The selective transport of proteins or lipids by vesicular transport is a fundamental process supporting cellular physiology. The budding process involves cargo sorting and vesicle formation at the donor membrane and constitutes an important process in vesicular transport. This process is particularly important for the polarized sorting in epithelial cells, in which the cargo molecules need to be selectively sorted and transported to two distinct destinations, the apical or basolateral plasma membrane. Adaptor protein (AP-1, a member of the AP complex family, which includes the ubiquitously expressed AP-1A and the epithelium-specific AP-1B, regulates polarized sorting at the trans-Golgi network and/or at the recycling endosomes. A growing body of evidence, especially from studies using model organisms and animals, demonstrates that the AP-1-mediated polarized sorting supports the development and physiology of multi-cellular units as functional organs and tissues (e.g., cell fate determination, inflammation and gut immune homeostasis. Furthermore, a possible involvement of AP-1B in the pathogenesis of human diseases, such as Crohn’s disease and cancer, is now becoming evident. These data highlight the significant contribution of AP-1 complexes to the physiology of multicellular organisms, as master regulators of polarized sorting in epithelial cells.

  3. The APS control system network

    International Nuclear Information System (INIS)

    The APS accelerator control system is a distributed system consisting of operator interfaces, a network, and computer-controlled interfaces to hardware. This implementation of a control system has come to be called the open-quotes Standard Model.close quotes The operator interface is a UNDC-based workstation with an X-windows graphical user interface. The workstation may be located at any point on the facility network and maintain full functionality. The function of the network is to provide a generalized communication path between the host computers, operator workstations, input/output crates, and other hardware that comprise the control system. The crate or input/output controller (IOC) provides direct control and input/output interfaces for each accelerator subsystem. The network is an integral part of all modem control systems and network performance will determine many characteristics of a control system. This paper will describe the overall APS network and examine the APS control system network in detail. Metrics are provided on the performance of the system under various conditions

  4. Ectopic expression of Jatropha curcas APETALA1 (JcAP1) caused early flowering in Arabidopsis, but not in Jatropha.

    Science.gov (United States)

    Tang, Mingyong; Tao, Yan-Bin; Xu, Zeng-Fu

    2016-01-01

    Jatropha curcas is a promising feedstock for biofuel production because Jatropha oil is highly suitable for the production of biodiesel and bio-jet fuels. However, Jatropha exhibits a low seed yield as a result of unreliable and poor flowering. APETALA1 (AP1) is a floral meristem and organ identity gene in higher plants. The flower meristem identity genes of Jatropha have not yet been identified or characterized. To better understand the genetic control of flowering in Jatropha, an AP1 homolog (JcAP1) was isolated from Jatropha. An amino acid sequence analysis of JcAP1 revealed a high similarity to the AP1 proteins of other perennial plants. JcAP1 was expressed in inflorescence buds, flower buds, sepals and petals. The highest expression level was observed during the early developmental stage of the flower buds. The overexpression of JcAP1 using the cauliflower mosaic virus (CaMV) 35S promoter resulted in extremely early flowering and abnormal flowers in transgenic Arabidopsis plants. Several flowering genes downstream of AP1 were up-regulated in the JcAP1-overexpressing transgenic plant lines. Furthermore, JcAP1 overexpression rescued the phenotype caused by the Arabidopsis AP1 loss-of-function mutant ap1-11. Therefore, JcAP1 is an ortholog of AtAP1, which plays a similar role in the regulation of flowering in Arabidopsis. However, the overexpression of JcAP1 in Jatropha using the same promoter resulted in little variation in the flowering time and floral organs, indicating that JcAP1 may be insufficient to regulate flowering by itself in Jatropha. This study helps to elucidate the function of JcAP1 and contributes to the understanding of the molecular mechanisms of flower development in Jatropha. PMID:27168978

  5. Preparing Students for the AP Psychology Exam

    Science.gov (United States)

    Whitlock, Kristin

    2013-01-01

    The Advanced Placement Psychology exam is one of the fastest growing exams offered by the College Board. The average percent of change in the number of students taking this exam over the past five years is 12.4%. With 238,962 students taking the exam in 2013, the AP Psychology exam is the sixth largest exam, surpassing AP Biology and AP World…

  6. An AP Calculus Classroom Amusement Park

    Science.gov (United States)

    Ferguson, Sarah

    2016-01-01

    Throughout the school year, AP Calculus teachers strive to teach course content comprehensively and swiftly in an effort to finish all required material before the AP Calculus exam. As early May approaches and the AP Calculus test looms, students and teachers nervously complete lessons, assignments, and assessments to ensure student preparation.…

  7. Testing of Solar Heated Domestic Hot Water System for Solahart Scandinavia ApS

    DEFF Research Database (Denmark)

    Andersen, Elsa

    1997-01-01

    The solar heating system marketed by Solahart Scandinavia ApS was tested in the Institutes test facility for SDHWsystems. The test results are described in the report.......The solar heating system marketed by Solahart Scandinavia ApS was tested in the Institutes test facility for SDHWsystems. The test results are described in the report....

  8. In vitro establishment of ivermectin-resistant Rhipicephalus microplus cell line and the contribution of ABC transporters on the resistance mechanism.

    Science.gov (United States)

    Pohl, Paula C; Carvalho, Danielle D; Daffre, Sirlei; Vaz, Itabajara da Silva; Masuda, Aoi

    2014-08-29

    The cattle tick Rhipicephalus microplus is one of the most economically damaging livestock ectoparasites, and its widespread resistance to acaricides is a considerable challenge to its control. In this scenario, the establishment of resistant cell lines is a useful approach to understand the mechanisms involved in the development of acaricide resistance, to identify drug resistance markers, and to develop new acaricides. This study describes the establishment of an ivermectin (IVM)-resistant R. microplus embryonic cell line, BME26-IVM. The resistant cells were obtained after the exposure of IVM-sensitive BME26 cells to increasing doses of IVM in a step-wise manner, starting from an initial non-toxic concentration of 0.5 μg/mL IVM, and reaching 6 μg/mL IVM after a 46-week period. BME26-IVM cell line was 4.5 times more resistant to IVM than the parental BME26 cell line (lethal concentration 50 (LC50) 15.1 ± 1.6 μg/mL and 3.35 ± 0.09 μg/mL, respectively). As an effort to determine the molecular mechanisms governing resistance, the contribution of ATP-binding cassette (ABC) transporter was investigated. Increased expression levels of ABC transporter genes were found in IVM-treated cells, and resistance to IVM was significantly reduced by co-incubation with 5 μM cyclosporine A (CsA), an ABC transporter inhibitor, suggesting the involvement of these proteins in IVM-resistance. These results are similar to those already described in IVM-resistant tick populations, and suggest that similar resistance mechanisms are involved in vitro and in vivo. They reinforce the hypothesis that ABC transporters are involved in IVM resistance and support the use of BME26-IVM as an in vitro approach to study acaricide resistance mechanisms. PMID:24956999

  9. Development and UHV qualification of aluminium alloy vacuum chambers for transport line-2 of CTF-3, CERN

    International Nuclear Information System (INIS)

    CLIC Test Facility (CTF-3) at CERN is a project to demonstrate the concept of high frequency RF generation for the particle acceleration using a drive electron beam. 63 numbers of Aluminium alloy A6061T6 vacuum chambers for transport line-2 (TL-2) of CTF-3 were developed in RRCAT under joint collaboration between RRCAT and CERN. These chambers have Race Track (RT) and Cylindrical (CY) cross section. RT and CY, both type straight chambers as well as bent chambers (for the dipole magnets) were required for the TL-2. There were 22 numbers RT straight chamber, 31 numbers CY straight chamber, 5 numbers RT Bent chamber, 3 numbers CY bent chambers and 2 numbers CY Bent spare chambers. UHV Section was responsible for the qualification of demountable joints made of A6061T6 and also responsible for exhaustive UHV testing of these chambers. During the development, 5 numbers of different type of prototype chambers were fabricated and rigorously tested for leak tightness, qualification of demountable joints and desired ultimate vacuum using number of baking cycles. Helium mass spectrometer leak detector was utilised for leak detection at various stages for insuring the leak tightness better than1x10-10 mbar-l/s. For ultimate vacuum testing three setups were developed for the three types of different vacuum chambers. Each test setup consisted of Sputter Ion Pump, Turbo Molecular Pump, BA Gauge and Residual Gas Analyser. All these UHV setups were qualified for their ultimate vacuum in 10-10 mbar range. The arrangements were made such that, 4 to 8 straight chambers could be assembled and tested together in single cycle on each setup. Similar chambers were assembled with special Diamond shaped Aluminium seals with optimised sealing torque and leak tightness of -11 mbar-l/s was ensured. The aluminium chambers were baked at 150℃ where as the SS setups were baked at 250℃ for 24 hours and ultimate vacuum in the range of 10-10 mbar was achieved in all the sixteen test cycles

  10. Transport inhibition of digoxin using several common P-gp expressing cell lines is not necessarily reporting only on inhibitor binding to P-gp.

    Directory of Open Access Journals (Sweden)

    Annie Albin Lumen

    Full Text Available We have reported that the P-gp substrate digoxin required basolateral and apical uptake transport in excess of that allowed by digoxin passive permeability (as measured in the presence of GF120918 to achieve the observed efflux kinetics across MDCK-MDR1-NKI (The Netherlands Cancer Institute confluent cell monolayers. That is, GF120918 inhibitable uptake transport was kinetically required. Therefore, IC50 measurements using digoxin as a probe substrate in this cell line could be due to inhibition of P-gp, of digoxin uptake transport, or both. This kinetic analysis is now extended to include three additional cell lines: MDCK-MDR1-NIH (National Institute of Health, Caco-2 and CPT-B2 (Caco-2 cells with BCRP knockdown. These cells similarly exhibit GF120918 inhibitable uptake transport of digoxin. We demonstrate that inhibition of digoxin transport across these cell lines by GF120918, cyclosporine, ketoconazole and verapamil is greater than can be explained by inhibition of P-gp alone. We examined three hypotheses for this non-P-gp inhibition. The inhibitors can: (1 bind to a basolateral digoxin uptake transporter, thereby inhibiting digoxin's cellular uptake; (2 partition into the basolateral membrane and directly reduce membrane permeability; (3 aggregate with digoxin in the donor chamber, thereby reducing the free concentration of digoxin, with concomitant reduction in digoxin uptake. Data and simulations show that hypothesis 1 was found to be uniformly acceptable. Hypothesis 2 was found to be uniformly unlikely. Hypothesis 3 was unlikely for GF120918 and cyclosporine, but further studies are needed to completely adjudicate whether hetero-dimerization contributes to the non-P-gp inhibition for ketoconazole and verapamil. We also find that P-gp substrates with relatively low passive permeability such as digoxin, loperamide and vinblastine kinetically require basolateral uptake transport over that allowed by +GF120918 passive permeability, while

  11. 77 FR 11192 - Fannin Rural Rail Transportation District-Lease and Operation Exemption-Line of Texas Department...

    Science.gov (United States)

    2012-02-24

    ... Paris to milepost 128.78 in Bonham, Tex. The line has been a part of Union Pacific Railroad Company's... extending between milepost 94.0, near Paris, and milepost 127.5, east of Bonham, in Lamar and Fannin Counties, Tex.\\1\\ After an agreement had been reached with UP for sale of the line but before...

  12. AP1000 Features Prevent Potential Containment Recirculation Screen Plugging

    International Nuclear Information System (INIS)

    This paper presents the results of plant design development and evaluations that demonstrate that the AP1000 plant is not subject to potential containment recirculation screen plugging following a loss-of-coolant-accident (LOCA). Following a LOCA in a pressurized water reactor, it is necessary to recirculate water from the containment back into the reactor to maintain long term core cooling. The AP1000 utilizes passive safety systems to provide containment recirculation for long term core cooling following a LOCA. The AP1000 also has non-safety pumps which provide a backup means of providing recirculation. Screens are provided around the recirculation pipes to prevent debris from blocking recirculation flow and core cooling passages. Debris may be generated by the LOCA blowdown from insulation and coatings used inside containment. Even with effective cleanliness programs, there may be some resident debris such as dust and dirt. The potential for plugging the recirculation screens is a current PWR licensing issue. The AP1000 design provides inherent advantages with respect to the potential plugging of containment recirculation screens. These characteristics include prevention of fibrous debris generation, improved debris settling and improved recirculation screen design. Debris settling analysis demonstrates that failure of coatings does not result in debris being transported to the screens before it settles to the floor. Additional analysis also shows that the plant can tolerate conservative amounts of resident debris being transported to the screens. The AP1000 significantly reduces the probability of plugging the containment recirculation screens and significantly reduces inspection and maintenance of coatings used inside containment. (authors)

  13. Transcellular transport of radioiodinated 3-iodo-α-methyl-L-tyrosine across monolayers of kidney epithelial cell line LLC-PK1

    International Nuclear Information System (INIS)

    3-[123I]iodo-α-methyl-L-tyrosine ([123I]IMT) is an imaging agent for amino acid transport. In order to obtain fundamental data related to tumor imaging with [123I]IMT and renal physiological accumulation of [123I]IMT, we investigated the transport characteristics of [125I]IMT in porcine kidney epithelial cell line LLC-PK1 using cell monolayers grown on microporous membrane filters. LLC-PK1 monolayers were created on a collagen-coated microporous (3 μm) membrane (4.7 cm2). To examine transcellular transport (secretion and reabsorption) and accumulation, the monolayers were incubated for up to 90 min at 37 deg C with 18.5 kBq [125I]IMT in Dulbecco's phosphate-buffered saline (pH 7.4) as an uptake solution. After incubation, transcellular transport was assessed by quantifying the radioactivity of the solutions on each side of the monolayer. For the accumulation experiment, the cells were solubilized in NaOH solution, and the radioactivity was quantified. For the inhibition experiment, the inhibitor was added at a final concentration of 1 mM. For the pH dependence experiment, the pH of the apical-side uptake solution was varied from pH 5 to pH 8. Transport of [14C]Tyr was examined for comparison. Bi-directional transcellular transport of [125I]IMT was observed, corresponding to secretion and reabsorption in proximal tubule. Accumulation of [125I]IMT from the basolateral side (1.62±0.15%) and the apical side (2.62±0.35%) was observed at 90 min. 2-Amino-bicyclo[2,2,1]heptane-2-carboxylic acid (a specific inhibitor of system L), L-Tyr (mother compound of [125I]IMT) and 2-aminoisobutyric acid (an inhibitor of system L and A) inhibited both directional transport (p125I]IMT from both sides (p125I]IMT transport is system L, rather than Na+-dependent transport, in both apical and basolateral membrane. [125I]IMT was transported by the system that transported L-Tyr, but the observed pH dependence of transport suggests that different mechanisms are involved in accumulation of

  14. APS high heat load monochromator

    International Nuclear Information System (INIS)

    This document contains the design specifications of the APS high heat load (HHL) monochromator and associated accessories as of February 1993. It should be noted that work is continuing on many parts of the monochromator including the mechanical design, crystal cooling designs, etc. Where appropriate, we have tried to add supporting documentation, references to published papers, and calculations from which we based our decisions. The underlying philosophy behind performance specifications of this monochromator was to fabricate a device that would be useful to as many APS users as possible, that is, the design should be as generic as possible. In other words, we believe that this design will be capable of operating on both bending magnet and ID beamlines (with the appropriate changes to the cooling and crystals) with both flat and inclined crystal geometries and with a variety of coolants. It was strongly felt that this monochromator should have good energy scanning capabilities over the classical energy range of about 4 to 20 keywith Si (111) crystals. For this reason, a design incorporating one rotation stage to drive both the first and second crystals was considered most promising. Separate rotary stages for the first and second crystals can sometimes provide more flexibility in their capacities to carry heavy loads (for heavily cooled first crystals or sagittal benders of second crystals), but their tuning capabilities were considered inferior to the single axis approach

  15. Berberine inhibits cyclin D1 expression via suppressed binding of AP-1 transcription factors to CCND1 AP-1 motif

    Institute of Scientific and Technical Information of China (English)

    Ye LUO; Yu HAO; Tai-ping SHI; Wei-wei DENG; Na LI

    2008-01-01

    Aim: To verify the suppressive effect of berberine on the proliferation of the human pulmonary giant cell carcinoma cell line PG and to demonstrate the mecha-nisms behind the antitumoral effects of berberine. Methods: The proliferative effects of PG cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetry. The cell cycle was examined by flow cytometry. The expression level of cyclin D1 was detected by RT-PCR. The activities of the activating protein-1 (AP-1) and NF-κB signaling pathways related to cyclin D1 were examined by luciferase assay. The cytoplasmic level of c-Jun was detected by Western blot analysis. An electrophoretic mobility shift assay was used to examiae the binding of transcription factors to the cyclin D1 gene (CCNDl) AP-1 motif. Results: The results showed that the proliferation of PG cells treated with different concentrations (10, 20, and 40 μg/mL) of berberine for 24 and 48 h was suppressed significantly compared to the control group. After treatment with berberine, the proportion of PG cells at the G0/G1 phase increased, while cells at the S and G2/M phases decreased. Berberine could inhibit the expression of cyclin D1 in PG cells. Berberine inhibited the activity of the AP-1 signaling pathway, but had no significant effect on the NF-κB signaling pathway. Berberine suppressed the expression of c-Jun and decreased the binding of tran-scription factors to the CCND1 AP-1 motif. Conclusion: Berberine suppresses the activity of the AP-1 signaling pathway and decreases the binding of transcrip-tion factors to the CCND1 AP-1 motif. This is one of the important mechanisms behind the antitumoral effects of berberine as a regulator of cyclin D1.

  16. Railroad Lines - RAIL_CROSSINGS_INDOT_IN: Railroad Crossings in Indiana (Indiana Department of Transportation, 1:1200, Point Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — RAIL_CROSSINGS_INDOT_IN is a point shapefile that contains locations of rail crossings in Indiana, provided by personnel of Indiana Department of Transportation...

  17. Constitutively internalized dopamine transporter is targeted to late endosomes and lysosomal degradation in heterologous cell lines and dopaminergic neurons

    DEFF Research Database (Denmark)

    Eriksen, Jacob; Madsen, Kenneth; Vægter, Christian Bjerggaard;

    of the single-membrane spanning protein Tac, thereby creating an extracellular antibody epitope. Upon expression in HEK293 cells this Tac-DAT fusion protein displayed uptake properties similar to the wild type (WT) transporter. Additionally, Tac-DAT was, like the WT, internalized both in response to PMA......The dopamine transporter (DAT) belongs to SLC6 family of Na+/Cl- coupled transporters and mediates clearance of released dopamine (DA) from the synaptic cleft. To investigate the constitutive trafficking of heterologously expressed DAT we fused the N-terminus of DAT to the intracellular tail...... and amphetamine, a substrate of the DAT. In antibody feeding experiments we observed that Tac-DAT was constitutively internalized faster than Tac alone and using an ELISA based assay we could quantify time-dependent intracellular accumulation of the transporter. Incubation with inhibitors of lysosomal degradation...

  18. Discovery of the longest-period rapidly oscillating Ap star HD177765

    CERN Document Server

    Alentiev, D; Ryabchikova, T; Cunha, M; Tsymbal, V; Weiss, W

    2011-01-01

    We present the discovery of a long-period, rapidly oscillating Ap star, HD177765. Using high-resolution time-series observations obtained with UVES at the ESO VLT telescope, we found radial velocity variations with amplitudes 7-150 m/s and a period of 23.6 min, exceeding that of any previously known roAp star. The largest pulsation amplitudes are observed for Eu III, Ce III and for the narrow core of Halpha. We derived the atmospheric parameters and chemical composition of HD177765, showing this star to be similar to other long-period roAp stars. Comparison with theoretical pulsational models indicates an advanced evolutionary state for HD177765. Abundance analyses of this and other roAp stars suggest a systematic variation with age of the rare-earth line anomalies seen in cool Ap stars.

  19. Dipeptide transport: an active process with energy- and proton-dependence in the human intestinal cell line, Caco-2

    Institute of Scientific and Technical Information of China (English)

    SUN Bing-wei; SHI Lei; CHEN Xi; CHEN Zhao-yong; TAI Ning-zheng; ZHAO Xiao-chen; LI Ning

    2007-01-01

    Objective: To determine the active process on dipeptide transport with proton- and energydependence in Caco-2 cells. Methods: A human intestinal cell monolayer (Caco-2) was used as the in vitro model of human small intestine and cephalexin as the model substrate for dipeptide transporter (PepT1). Caco-2 cells grown on multiwell dishes (24 wells) and Transwell membrane filters were incubated in the culture medium. The transport and uptake experiments of cephalexin across apical membranes were then conducted with different temperature and different pH values. Uptake of cephalexin in Caco-2 cells grown on multiple well dishes with addition of energy inhibitors( sodium azide, SA and 2,4-dinitrophenol, DNP) were then measured. Results: The accumulation of cephalexin into Caco-2 monolayers increased with the duration of culture. The uptake from the apical surface was markedly influenced by the pH of the apical medium, and the maximal uptake was achieved at pH 5.5; further acidification of the incubation medium may decrease transport of cephalexin despite an increase inward H + gradient.Cephalexin uptake was linear over the concentration range when the cells were incubated at 4 ℃ while the uptake rate was enhanced and tended to be saturated as the cephalexin concentration increased when the cells were incubated at 37 ℃. The kinetic parameters for the cephalexin transport carrier were determined to be: Vmax of (22. 173 ±1.9) nmol/min per mg protein, Km of (2.069 ±0.9) mol/L, the Kd was estimated to be (0.07 ± 0.02) nmol/min per mg protein per mmol/L. Uptake of cephalexin was markedly inhibited by sodium azide (SA) and 2,4-dinitrophenol (DNP). Conclusion: Cephalexin was transported actively across Caco-2 cells, and the transport process was proton- and energy-dependent. In addition, Caco-2 cells taked up cephalexin by dipeptide transporters that closely resembled the transporters present in the intestine. Caco-2 cells represented an ideal cellular model for future

  20. On AP-property of Function Spaces

    Institute of Scientific and Technical Information of China (English)

    SHEN Rong-xin

    2012-01-01

    In this paper,it is discussed the AP-property of function spaces.We prove that for any compact network α for a space X which is closed under finite unions,(1) if Cα(X) is an AP-space and X is paracompact,then X is a Hurewicz space; (2) if Cα (X) is an AP-space which has countable tightness,then Cα(X) is discretely generated.

  1. Transportation infrastructure between nuclear power plant gates and nearest line-haul networks: Plan and procedure for data development

    International Nuclear Information System (INIS)

    This study is concerned with the segments of the transportation system that include possible routings over public roads and private (or public) rail links, and waterway access (within 25 miles) from the gates of typical reactor sites to proximate links of what can be termed the national through-route system. These routings are by no means uniform throughout the United States. Local roads and rail links near reactor sites may be subject to a wide variety of jurisdictions for maintenance, repair, and inspection; may or may not (at present) qualify for federal assistance under the Federal-Aid Highway and related funding programs; may or may not meet accepted construction standards for facilities expected to bear heavy loads; and, perhaps most importantly for the spent-fuel transportation program, may be subject to occasional and currently unavoidable disruptions that could seriously impair shipment schedules. The overall objectives of the study are to describe a framework for identifying the characteristics of the near-site transportation networks of all existing nuclear power plants that could give rise to important shipment scheduling and programming constraints. These characteristics cover both transportation infrastructure and existing structural and environmental limitation, and define a scope and schedule for constructing a data base for the transportation networks surrounding all nuclear power plants. 6 refs., 4 figs., 8 tabs

  2. AP calculus AB & BC crash course

    CERN Document Server

    Rosebush, J

    2012-01-01

    AP Calculus AB & BC Crash Course - Gets You a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. AP Calculus AB & BC Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know Crash Course is based on an in-depth analysis of the AP Calculus AB & BC course description outline and actual AP test questions. It covers only the information tested on the exams, so you can make the most of your valuable study time. Written by experienced math teachers, our

  3. Vision document on transport scarcity. Policy line for shortage in transport capacity on the electricity grid; Visiedocument transportschaarste. Beleidslijn inzake de schaarste aan transportcapaciteit op het elektriciteitsnetwerk

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-01-15

    The Netherlands Competition Authority (NMa) has observed an increasing problem in relation to possible future shortage of transport capacity in the electricity grid. This vision document aims to provide clarity to market parties with regard to their general vision on this shortage problem [mk]. [Dutch] De Nederlandse Mededingingsautoriteit (NMa) heeft geconstateerd dat er in toenemende mate problemen optreden die verband houden met de (mogelijk toekomstige) schaarste aan transportcapaciteit op het elektriciteitsnetwerk.Met dit Visiedocument beoogt de NMa zoveel mogelijk duidelijkheid te verschaffen aan marktpartijen met betrekking tot zijn algemene visie ten aanzien van de schaarsteproblematiek.

  4. Off-line algorithm for calculation of vertical tracer transport in the troposphere due to deep convection

    Directory of Open Access Journals (Sweden)

    D. A. Belikov

    2013-02-01

    Full Text Available A modified cumulus convection parametrisation scheme is presented. This scheme computes the mass of air transported upward in a cumulus cell using conservation of moisture and a detailed distribution of convective precipitation provided by a reanalysis dataset. The representation of vertical transport within the scheme includes entrainment and detrainment processes in convective updrafts and downdrafts. Output from the proposed parametrisation scheme is employed in the National Institute for Environmental Studies (NIES global chemical transport model driven by JRA-25/JCDAS reanalysis. The simulated convective precipitation rate and mass fluxes are compared with observations and reanalysis data. A simulation of the short-lived tracer 222Rn is used to further evaluate the performance of the cumulus convection scheme. Simulated distributions of 222Rn are evaluated against observations at the surface and in the free troposphere, and compared with output from models that participated in the TransCom-CH4 Transport Model Intercomparison. From this comparison, we demonstrate that the proposed convective scheme in general is consistent with observed and modeled results.

  5. Off-line algorithm for calculation of vertical tracer transport in the troposphere due to deep convection

    Directory of Open Access Journals (Sweden)

    D. A. Belikov

    2012-08-01

    Full Text Available A modified cumulus convection parametrisation scheme is presented. This scheme computes the mass of air transported upward in a cumulus cell using conservation of moisture and a detailed distribution of convective precipitation provided by a reanalysis dataset. The representation of vertical transport within the scheme includes entrainment and detrainment processes in convective updrafts and downdrafts. Output from the proposed parametrisation scheme is employed in the National Institute for Environmental Studies (NIES global chemical transport model driven by JRA-25/JCDAS reanalysis. The simulated convective precipitation rate and mass fluxes are compared with observations and reanalysis data. A simulation of the short-lived tracer 222Rn is used to further evaluate the performance of the cumulus convection scheme. Simulated distributions of 222Rn are validated against observations at the surface and in the free troposphere, and compared with output from models that participated in the TransCom-CH4 Transport Model Intercomparison. From this comparison, we demonstrate that the proposed convective scheme can successfully reproduce deep cloud convection.

  6. Kinetics and conductivity parameters of uptake and transport of polychlorinated biphenyls in the Caco-2 intestinal cell line model

    NARCIS (Netherlands)

    Dulfer, W.J.; Govers, H.A.J.; Groten, J.P.

    1998-01-01

    Most of the accumulation of polychlorinated biphenyls (PCBs) over the food chain can be attributed to contaminant uptake from food. The effect of fatty acid absorption on net uptake and transport fluxes of a selection of 14 PCBs over the organismal gut epithelium has been determined in monolayers of

  7. Transport characteristics of L-carnosine and the anticancer derivative 4-toluenesulfonylureido-carnosine in a human epithelial cell line

    DEFF Research Database (Denmark)

    Nielsen, Carsten Uhd; Supuran, Claudiu T; Scozzafava, Andrea;

    2002-01-01

    The aim of the present study was to evaluate whether the transepithelial transport of the anticancer compound 4-toluenesulfonylureido-carnosine (Ts-carnosine) and the dipeptide moiety L-carnosine was due to a hPepT1 carrier-mediated flux....

  8. Predicting gemcitabine transport and toxicity in human pancreatic cancer cell lines with the positron emission tomography tracer 3'-deoxy-3'-fluorothymidine.

    Science.gov (United States)

    Paproski, Robert J; Young, James D; Cass, Carol E

    2010-02-15

    The abundance of human equilibrative nucleoside transporter 1 (hENT1) has recently been shown to be a predictive marker of benefit from gemcitabine therapy in patients with pancreatic cancer. Since hENT1 is also important for the uptake of positron emission tomography (PET) tracer 3'-deoxy-3'-fluorothymidine (FLT) in various cultured human cell lines, this study was undertaken to determine if FLT uptake predicts gemcitabine uptake and/or toxicity in a panel of human pancreatic cancer cell lines (Capan-2, AsPC-1, BxPC-3, PL45, MIA PaCa-2, and PANC-1). Capan-2 cells displayed the lowest levels of (1) extracellular nitrobenzylmercaptopurine ribonucleoside (NBMPR) binding, which represents cell-surface hENT1, (2) FLT and gemcitabine uptake during short (1-45s) and prolonged (1h) periods, and (3) gemcitabine sensitivity. Exposure to NBMPR (inhibits only hENT1) or dilazep (inhibits hENT1 and hENT2) reduced FLT and gemcitabine uptake and gemcitabine sensitivity, with dilazep having greater effects than NBMPR. Gemcitabine permeation was almost completely mediated, primarily by hENT1 and to a lesser extent by hENT2, whereas FLT permeation included a substantial component of passive diffusion. In five of six cell lines, correlations were observed between (1) FLT and gemcitabine initial rates of uptake, (2) gemcitabine uptake and gemcitabine toxicity, (3) FLT uptake and gemcitabine toxicity, and (4) ribonucleotide reductase subunit M1 expression and gemcitabine toxicity. FLT and gemcitabine uptake were comparable for predicting gemcitabine toxicity in the tested pancreatic cancer cell lines suggesting that FLT PET may provide clinically useful information about tumor gemcitabine transport capacity and sensitivity. PMID:19788890

  9. Analysis of high iron rice lines reveals new miRNAs that target iron transporters in roots

    Science.gov (United States)

    Paul, Soumitra; Gayen, Dipak; Datta, Swapan K.; Datta, Karabi

    2016-01-01

    The present study highlights the molecular regulation of iron transport in soyFER1-overexpressing transgenic rice. Accumulation of iron in three different seed developmental stages, milk, dough, and mature, has been examined. The transgenic seeds of the milk stage showed significant augmentation of iron and zinc levels compared with wild-type seeds, and similar results were observed throughout the dough and mature stages. To investigate the regulation of iron transport, the role of miRNAs was studied in roots of transgenic rice. Sequencing of small RNA libraries revealed 153 known and 41 novel miRNAs in roots. Among them, 59 known and 14 novel miRNAs were found to be significantly expressed. miR166, miR399, and miR408 were identified as playing a vital role in iron uptake in roots of transgenic plants . Most importantly, four putative novel miRNAs, namely miR11, miR26, miR30, and miR31, were found to be down-regulated in roots of transgenic plants. For all these four novel miRNAs, natural resistance-associated macrophage protein 4 (NRAMP4), encoding a metal transporter, was predicted as a target gene. It is hypothesized that the NRAMP4 transporter is activated in roots of transgenic plants due to the lower abundance of its corresponding putative novel miRNAs. The relative transcript level of the NRAMP4 transcript was increased from 0.107 in the wild type to 65.24 and 55.39 in transgenic plants, which demonstrates the elevated amount of iron transport in transgenic plants. In addition, up-regulation of OsYSL15, OsFRO2, and OsIRT1 in roots also facilitates iron loading in transgenic seeds. PMID:27729476

  10. Mapping of the spontaneous deletion in the Ap3d1 gene of mocha mice: fast and reliable genotyping

    OpenAIRE

    Delenclos Marion; Holm Mai; Drasbek Kim; Jensen Kimmo

    2008-01-01

    Abstract Background The mocha mouse carries a spontaneous deletion in the Ap3d1 gene, encoding the delta 1 subunit of the adaptor related protein complex 3, (Ap3d1), and subsequently lack the expression of functional AP-3. This leads to a deficiency in vesicle transport and storage, which affects neurotransmitter vesicle turnover and release in the central nervous system. Since the genomic sequence of the Ap3d1 gene of mocha mouse is not known, precise mapping of the deletion as well as relia...

  11. Stokes IQUV mapping of $\\alpha^2$ CVn & other Ap stars using ESPaDOnS and NARVAL

    OpenAIRE

    Silvester, J.; Kochukhov, O.; Wade, G. A.

    2013-01-01

    New spectral line polarisation observations of 7 bright Ap stars have been obtained with the ESPaDOnS and Narval high resolution spectropolarimeters (Silvester et al. 2012). The aim of this data set is produce a series of surface magnetic field and surface chemistry maps for these Ap stars. We present new magnetic maps for the Ap star $\\alpha^2$ CVn using these new data and the MDI inversion code INVERS10. $\\alpha^2$ CVn is the first Ap star to be observed during two separate epochs using hig...

  12. Coaching Strategies for AP: Building a Successful AP European History Program

    Science.gov (United States)

    Fornaciari, Jim

    2014-01-01

    The October 2013 special issue of "Social Education" dealt with almost all AP social studies subjects, but omitted AP European History. This is one of the most fascinating AP subjects for students and teachers alike. In this article, the author shares his experiences since hewas given the responsibility of building his school's…

  13. Installation Technology of AP1000 Steam Generator%AP1000蒸汽发生器安装技术

    Institute of Scientific and Technical Information of China (English)

    郑东宏; 王志强; 张明; 门昌华; 胡德全

    2013-01-01

    AP1000 nuclear power plant is a 3rd generation advanced passive 1000 MWe pressurized water reactor plant with two loops of reactor coolant systems .Compared with the conventional 2nd generation pressurized water reactor technology ,the steam generator ( SG) of AP1000 has many improved changes concerning SG property features and arrangement requirements for facilities within the nuclear island . Based on these improved changes and relevant installation practices ,the installation technology of AP 1000 SG has been introduced and analyzed including installation preparation ,transportation ,lifting,and instal-lation construction ,etc., with a view to provide a technical reference to the installation of main equip-ments in steam supply system of nuclear power plant .%AP1000是二环路的1000 MWe级压水堆三代核电厂,采用非能动安全设施,与传统的二代压水堆核电技术相比,蒸汽发生器本体特征和核岛内的就位要求均有较大的改进。根据AP1000蒸汽发生器自身的特点,并在实践的基础上,对蒸汽发生器的现场安装技术,包括安装准备、现场运输、吊装、核岛安装等进行了介绍和分析,为核电站核蒸汽供应系统主设备的现场运输、吊装和安装提供技术参考。

  14. AP Courses Get Audited for Quality

    Science.gov (United States)

    Ashford, Ellie

    2007-01-01

    As the college admissions process has gotten much more competitive, the number of high school students taking Advanced Placement (AP) courses has soared. At the same time, policymakers and education leaders seek to get more minorities and students not on the college track to sign up for AP and other rigorous classes. But as high schools have…

  15. AP-42 ADDITIONS AND REVISIONS - WILDLAND FIRES

    Science.gov (United States)

    This project is aimed at consolidating, selecting, and disseminating the most appropriate data and methods for estimating air emissions from wildfires and prescribed burns. The product will replace a current section of AP-42, but may not take the precise form of an AP-42 secti...

  16. The status of APS, BESSRC, and NEET.

    Energy Technology Data Exchange (ETDEWEB)

    Ahmad, I.; Dunford, R. W.; Esbensen, H.; Gemmell, D. S.; Kanter, E. P.; Kraessig, B.; Rutt, U.; Southworth, S. H.

    1999-03-10

    We present a brief summary of the current status of the Advanced Photon Source (APS) at Argonne National Laboratory and of the facilities at two of the APS sectors operated by the Basic Energy Sciences Synchrotrons Radiation Center (BESSRC). This is followed by a report on recent measurements at BESSRC on the phenomenon of Nuclear Excitation by Electronic Transition (NEET).

  17. Residual radiation studies at AP0

    Energy Technology Data Exchange (ETDEWEB)

    Alexander J Elwyn et al.

    2002-06-19

    The radiation environment at the NuMI experiment has received a lot of attention in the last few years in preparation for project construction. One important issue is the induced radioactivation of the components in the beam line and the shielding materials. This arises from irradiation by hadrons that are generated in the target. Since the level of the residual activity has to be considered when determining access procedures for maintenance during NuMI operation, an understanding of the properties of the remnant radiation is important. To this end, experimental studies were performed in the target vault at AP0 which is similar in design to the NuMI target area. Here 120 GeV protons bombard a target, generating the hadrons that produce the induced radioactivity. Two sets of samples each consisting of three small cylindrical or rectangular solids of iron and steel, one sample of aluminum, and one of concrete were irradiated. One set was hung just below the bottom of a module near the lithium lens (in-vault), and the other was placed on top of the modules downstream of this location (above-vault), just beneath the movable concrete roof of the vault at AP0. Further, four thin activation foils of Au, Au+Cd, In, and Al (along with small disks of the same iron, aluminum, and concrete samples discussed above) were mounted on four 10.2 cm diameter Al disks, one placed on the in-vault module and three at above-vault downstream locations as well. The radioactivity of all these materials on the 10.2 cm Al disks was determined at the Radioisotope Analysis Facility in an attempt to characterize the radionuclides produced during irradiation. The activities of the thin foils were employed in an effort to unfold a spectrum of the neutrons produced during the hadronic cascades in the target. The MARS Monte Carlo code (MO95, MO00) was used to predict and analyze the residual radiation produced during the beam irradiation. New subroutines have been developed for the MARS14 version

  18. APS undulator radiation: First results

    International Nuclear Information System (INIS)

    The first undulator radiation has been extracted from the Advanced Photon Source (APS). The results from the characterization of this radiation are very satisfactory. With the undulator set at a gap of 15.8 mm (K=1.61), harmonics as high as the 17th were observed using a crystal spectrometer. The angular distribution of the third-harmonic radiation was measured, and the source was imaged using a zone plate to determine the particle beam emittance. The horizontal beam emittance was found to be 6.9 ± 1.0 nm-rad, and the vertical emittance coupling was found to be less than 3%. The absolute spectral flux was measured over a wide range of photon energies, and it agrees remarkably well with the theoretical calculations based on the measured undulator magnetic field profile and the measured beam emittance. These results indicate that both the emittance of the electron beam and the undulator magnetic field quality exceed the original specifications

  19. Unsaturated fatty acids and phytosterols regulate cholesterol transporter genes in Caco-2 and HepG2 cell lines.

    Science.gov (United States)

    Park, Youngki; Carr, Timothy P

    2013-02-01

    Dietary consumption of phytosterols and certain fatty acids has been shown to reduce cholesterol absorption and plasma cholesterol concentrations. However, it has not been fully elucidated whether phytosterols or fatty acids can alter the expression of cholesterol transporters by functioning as signaling molecules. This study tested the hypothesis that various fatty acids and phytosterols commonly found in the food supply can modulate the expression of transporters including Niemann-Pick C1-like 1, low-density lipoprotein receptor, and scavenger receptor class B type I and 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the intestine and liver. Caco-2 cells were used as models of enterocytes, and HepG2 cells were used as a model of hepatocytes. The cells were treated for 18 hours with 100 μmol/L of a fatty acid, or for 24 hours with 10 μmol/L of 25α-hydroxycholesterol, or 100 μmol/L of cholesterol, sitosterol, and stigmasterol to measure expression of genes involved in cholesterol transport using quantitative real-time polymerase chain reaction. Polyunsaturated fatty acids in Caco-2 cells and sterols in HepG2 cells significantly reduced the messenger RNA expression levels of Niemann-Pick C1-like 1, scavenger receptor class B type I, low-density lipoprotein receptor, and 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Importantly, sitosterol and stigmasterol reduced the messenger RNA levels of genes to a similar extent as cholesterol. The data support the hypothesis that unsaturated fatty acid and phytosterols can act as signaling molecules and alter the expression of genes involved in cholesterol transport and metabolism.

  20. Alaska-US gas line - design considerations for the Alaska segment of ANGTS (Alaska natural gas transportation system)

    Energy Technology Data Exchange (ETDEWEB)

    Hetland, N.

    1982-01-01

    In 1968, the largest single discovery of oil and natural gas ever found on the North American continent was made at Prudhoe Bay on the North Slope of Alaska. The Prudhoe Bay field contains over 26 tcf of recoverable natural gas, or ca 13% of the proven domestic gas reserves. To bring this natural gas to the market in the Lower 48 states, filings were made with the Federal Power Commission, the predecessor to the Federal Energy Regulatory Commission, to construct a pipeline transportation system. The gas pipeline project will initially transport ca 2 billion cu ft of gas daily, expandable to 3.2 billion cu ft/day with additional compressor stations. The total ANGTS comprises nearly 4,800 miles of pipeline with diameters ranging from 36 to 56 in., and initially ca 1.4 million hp will be installed to transport 2.0 billion cu ft/day. This presentation concentrates on the Alaska segment of the ANGTS.

  1. Quantitative analysis of photoquadrats acquired at Johnston Atoll permanent transect site 1A-P on June 29, 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This spreadsheet summarizes the number of corals photographed along a permanent transect line at Underwater Site 1A-P at Johnston Atoll on 6/29/2000.

  2. Quantitative analysis of photoquadrats acquired at Johnston Atoll permanent transect site 2A-P on June 30, 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This spreadsheet summarizes the number of corals photographed along a permanent transect line at Underwater Site 2AP at Johnston Atoll on 6302000.

  3. Quantitative analysis of photoquadrats acquired at Johnston Atoll permanent transect site 3A-P on July 1, 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This spreadsheet summarizes the number of corals photographed along a permanent transect line at Underwater Site 3AP at Johnston Atoll on 712000.

  4. Parametric Study of the current limit within a single driver-scale transport beam line of an induction Linac for Heavy Ion Fusion

    International Nuclear Information System (INIS)

    The High Current Experiment (HCX) at Lawrence Berkeley National Laboratory is part of the US program that explores heavy-ion beam as the driver option for fusion energy production in an Inertial Fusion Energy (IFE) plant. The HCX is a beam transport experiment at a scale representative of the low-energy end of an induction linear accelerator driver. The primary mission of this experiment is to investigate aperture fill factors acceptable for the transport of space-charge-dominated heavy-ion beams at high intensity (line charge density ∼0.2 (micro)C/m) over long pulse durations (4 (micro)s) in alternating gradient focusing lattices of electrostatic or magnetic quadrupoles. This experiment is testing transport issues resulting from nonlinear space-charge effects and collective modes, beam centroid alignment and steering, envelope matching, image charges and focusing field nonlinearities, halo and, electron and gas cloud effects. We present the results for a coasting 1 MeV K+ ion beam transported through ten electrostatic quadrupoles. The measurements cover two different fill factor studies (60% and 80% of the clear aperture radius) for which the transverse phase-space of the beam was characterized in detail, along with beam energy measurements and the first halo measurements. Electrostatic quadrupole transport at high beam fill factor (∼80%) is achieved with acceptable emittance growth and beam loss. We achieved good envelope control, and re-matching may only be needed every ten lattice periods (at 80% fill factor) in a longer lattice of similar design. We also show that understanding and controlling the time dependence of the envelope parameters is critical to achieving high fill factors, notably because of the injector and matching section dynamics

  5. Electron stripping processes of H- ion beam in the 80 kV high voltage extraction column and low energy beam transport line at LANSCE

    Science.gov (United States)

    Draganic, I. N.

    2016-02-01

    Basic vacuum calculations were performed for various operating conditions of the Los Alamos National Neutron Science H- Cockcroft-Walton (CW) injector and the Ion Source Test Stand (ISTS). The vacuum pressure was estimated for both the CW and ISTS at five different points: (1) inside the H- ion source, (2) in front of the Pierce electrode, (3) at the extraction electrode, (4) at the column electrode, and (5) at the ground electrode. A static vacuum analysis of residual gases and the working hydrogen gas was completed for the normal ion source working regime. Gas density and partial pressure were estimated for the injected hydrogen gas. The attenuation of H- beam current and generation of electron current in the high voltage acceleration columns and low energy beam transport lines were calculated. The interaction of H- ions on molecular hydrogen (H2) is discussed as a dominant collision process in describing electron stripping rates. These results are used to estimate the observed increase in the ratio of electrons to H- ion beam in the ISTS beam transport line.

  6. Electron stripping processes of H− ion beam in the 80 kV high voltage extraction column and low energy beam transport line at LANSCE

    International Nuclear Information System (INIS)

    Basic vacuum calculations were performed for various operating conditions of the Los Alamos National Neutron Science H− Cockcroft-Walton (CW) injector and the Ion Source Test Stand (ISTS). The vacuum pressure was estimated for both the CW and ISTS at five different points: (1) inside the H− ion source, (2) in front of the Pierce electrode, (3) at the extraction electrode, (4) at the column electrode, and (5) at the ground electrode. A static vacuum analysis of residual gases and the working hydrogen gas was completed for the normal ion source working regime. Gas density and partial pressure were estimated for the injected hydrogen gas. The attenuation of H− beam current and generation of electron current in the high voltage acceleration columns and low energy beam transport lines were calculated. The interaction of H− ions on molecular hydrogen (H2) is discussed as a dominant collision process in describing electron stripping rates. These results are used to estimate the observed increase in the ratio of electrons to H− ion beam in the ISTS beam transport line

  7. Dynamic analysis of Arabidopsis AP2 σ subunit reveals a key role in clathrin-mediated endocytosis and plant development.

    Science.gov (United States)

    Fan, Lusheng; Hao, Huaiqing; Xue, Yiqun; Zhang, Liang; Song, Kai; Ding, Zhaojun; Botella, Miguel A; Wang, Haiyang; Lin, Jinxing

    2013-09-01

    Clathrin-mediated endocytosis, which depends on the AP2 complex, plays an essential role in many cellular and developmental processes in mammalian cells. However, the function of the AP2 complex in plants remains largely unexplored. Here, we show in Arabidopsis that the AP2 σ subunit mutant (ap2 σ) displays various developmental defects that are similar to those of mutants defective in auxin transport and/or signaling, including single, trumpet-shaped and triple cotyledons, impaired vascular pattern, reduced vegetative growth, defective silique development and drastically reduced fertility. We demonstrate that AP2 σ is closely associated and physically interacts with the clathrin light chain (CLC) in vivo using fluorescence cross-correlation spectroscopy (FCCS), protein proximity analyses and co-immunoprecipitation assays. Using variable-angle total internal reflection fluorescence microscopy (VA-TIRFM), we show that AP2 σ-mCherry spots colocalize with CLC-EGFP at the plasma membrane, and that AP2 σ-mCherry fluorescence appears and disappears before CLC-EGFP fluorescence. The density and turnover rate of the CLC-EGFP spots are significantly reduced in the ap2 σ mutant. The internalization and recycling of the endocytic tracer FM4-64 and the auxin efflux carrier protein PIN1 are also significantly reduced in the ap2 σ mutant. Further, the polar localization of PIN1-GFP is significantly disrupted during embryogenesis in the ap2 σ mutant. Taken together, our results support an essential role of AP2 σ in the assembly of a functional AP2 complex in plants, which is required for clathrin-mediated endocytosis, polar auxin transport and plant growth regulation. PMID:23924631

  8. Development of Superconducting Combined Function Magnets for the Proton Transport Line for the J-PARC Neutrino Experiments

    CERN Document Server

    Nakamoto, Tatsushi; Anerella, Michael; Escallier, John; Fujii, T; Fukui, Yuji; Ganetis, George; Gupta, Ramesh C; Harrison, Michael; Hashiguchi, E; Higashi, Norio; Ichikawa, Atsuko; Iwamoto, Yosuke; Jain, Animesh K; Kanahara, T; Kimura, Nobuhiro; Kobayashi, Takashi; Makida, Yasuhiro; Muratore, Joseph F; Obana, Tetsuhiro; Ogitsu, T; Ohhata, Hirokatsu; Okamura, T; Orikasa, T; Parker, Brett; Sasaki, Ken Ichi; Takasaki, Minoru; Tanaka, Ken Ichi; Terashima, Akio; Tomaru, Takayuki; Wanderer, Peter; Yamamoto, Akira

    2005-01-01

    A second generation of long-baseline neutrino oscillation experiments has been proposed as one of the main projects at J-PARC jointly built by JAERI and KEK. Superconducting combined function magnets, SCFMs, will be utilized for the 50 GeV, 750 kW proton beam line for the neutrino experiment and an R&D program is in underway at KEK. The magnet is designed to provide a combined function of a dipole field of 2.6 T with a quadrupole field of 19 T/m in a coil aperture of 173.4 mm. A series of 28 magnets in the beam line will be operated DC in supercritical helium cooling below 5 K. A design feature of the SCFM is the left-right asymmetry of the coil cross section: current distributions for superimposed dipole- and quadrupole- fields are combined in a single layer coil. Another design feature is the adoption of glass-fiber reinforced phenolic plastic spacers to replace the conventional metallic collars. To evaluate this unique design, fabrication of full-scale prototype magnets is in progress at KEK and the fi...

  9. Altered retinal cell differentiation in the AP-3 delta mutant (Mocha) mouse.

    Science.gov (United States)

    Baguma-Nibasheka, Mark; Kablar, Boris

    2009-11-01

    Adaptor-related protein complex 3 delta 1 (Ap3d1) encodes the delta 1 subunit of an adaptor protein regulating intracellular vesicle-mediated transport, and the Ap3d-deletion mutant (Mocha) mouse undergoes rapid photoreceptor degeneration leading to blindness soon after birth. Previous microarray analysis revealed Ap3d down-regulation in the retina of mouse embryos specifically lacking cholinergic amacrine cells as a result of the absence of skeletal musculature. To investigate the role of Ap3d in the determination of retinal cell fate, the present study examined retinal morphology in newborn Ap3d-/- mice. The Ap3d-/- retina showed a complete absence of cholinergic amacrine cells and a decrease in parvalbumin-expressing amacrine cells and syntaxin- and VC1.1-expressing amacrine precursor cells, but had a normal number of cell layers and number of cells in each layer with no detectable difference in cell proliferation or apoptosis. These findings indicate that, despite having no apparent effect on the basic spatial organization of the retina at this stage of development, Ap3d could be involved in the regulation of progenitor cell competence and the eventual ratio of resulting differentiated cells. Finding the mouse mutant which phenocopies the eye defect seen in fetuses with no striated muscle was accomplished by the Systematic Subtractive Microarray Analysis Approach (SSMAA), explained in the discussion section. PMID:19631730

  10. Tank 241-AP-107, grab samples, 7AP-99-1, 7AP-99-3 and 7AP-99-4 analytical results for the final report

    International Nuclear Information System (INIS)

    This document is the format IV, final report for the tank 241-AP-107 (AP-107) grab samples taken in May 1999 to address waste compatibility concerns. Chemical, radiochemical, and physical analyses on the tank AP-107 samples were performed as directed in Compatibility Grab Sampling and Analysis Plan for Fiscal year 1999. Any deviations from the instructions provided in the tank sampling and analysis plan (TSAP) were discussed in this narrative. Interim data were provided earlier to River Protection Project (RPP) personnel, however, the data presented here represent the official results. No notification limits were exceeded

  11. Tank 241-AP-107, grab samples, 7AP-99-1, 7AP-99-3 and 7AP-99-4 analytical results for the final report

    Energy Technology Data Exchange (ETDEWEB)

    BELL, K.E.

    1999-08-12

    This document is the format IV, final report for the tank 241-AP-107 (AP-107) grab samples taken in May 1999 to address waste compatibility concerns. Chemical, radiochemical, and physical analyses on the tank AP-107 samples were performed as directed in Compatibility Grab Sampling and Analysis Plan for Fiscal year 1999. Any deviations from the instructions provided in the tank sampling and analysis plan (TSAP) were discussed in this narrative. Interim data were provided earlier to River Protection Project (RPP) personnel, however, the data presented here represent the official results. No notification limits were exceeded.

  12. AP English language & composition crash course

    CERN Document Server

    Hogue, Dawn

    2012-01-01

    AP English Language & Composition Crash Course - Gets You a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. AP English Language & Composition Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know Crash Course is based on an in-depth analysis of the AP English Language & Composition course description outline and actual Advanced Placement test questions. It covers only the information tested on the exam, so you can make the most of your valua

  13. Standardized safety management of AP1000 nuclear power plant

    International Nuclear Information System (INIS)

    In 2002, China published and implemented the Law of the People's Republic of China on Work Safety and promulgated a series of guidelines and policies, which strengthened the safety management supervision. Standardization of safety, as another important step on safety supervision, comes after safety assesment and safety production licensing system, is also a permanent solution. Standardization of safety is a strategic, long term and fundamental work, which is also the basic access to achieving scientific safety management and increasing the inherent safety of an enterprise. Haiyang AP1000 nuclear power plant, adopting the modularized, 'open-top' and parallel construction means, overturned the traditional construction theory of installation work comes after the civil work and greatly shorten the construction period. At the same time, the notable increase of oversize module transportation and lifting and parallel construction raises higher demands for safety management. This article combines the characteristics and difficulties of safety management for Haiyang AP1000 nuclear power plant, puts forward ideas and methods for standardized safety management, and could also serve as reference to the safety management for other AP1000 projects. (authors)

  14. Inhibition of ALDH1A1 activity decreases expression of drug transporters and reduces chemotherapy resistance in ovarian cancer cell lines.

    Science.gov (United States)

    Januchowski, Radosław; Wojtowicz, Karolina; Sterzyńska, Karolina; Sosińska, Patrycja; Andrzejewska, Małgorzata; Zawierucha, Piotr; Nowicki, Michał; Zabel, Maciej

    2016-09-01

    The high mortality of ovarian cancer patients results from the failure of treatment caused by the inherent or acquired chemotherapy drug resistance. It was reported that overexpression of aldehyde dehydrogenase A1 (ALDH1A1) in cancer cells can be responsible for the development of drug resistance. To add the high expression of the drug transporter proteins the ALDHA1 is considered as a molecular target in cancer therapy. Therefore, we analysed drug-resistant ovarian cancer cell lines according to ALDHA1 expression and the association with drug resistance. The expression of ALDH1A1, P-glycoprotein (P-gp) or breast cancer resistance protein (BCRP) was determined using a microarray and confirmed by Q-PCR, western blot and fluorescence analysis. ALDH1A1 activity was determined using an Aldefluor assay. The impact of all-trans retinoic acid (ATRA) and diethylaminobenzaldehyde (DEAB) on chemotherapy resistance was assessed by the MTT chemosensitivity assay. The most abundant expression of ALDH1A1 was noted in paclitaxel- and topotecan-resistant cell lines where two populations of ALDH-positive and ALDH-negative cells could be observed. Those cell lines also revealed the overexpression of P-gp and BCRP respectively, and were able to form spheres in non-adherent conditions. Pre-treatment with ATRA and DEAB reduced chemotherapy resistance in both cell lines. ATRA treatment led to downregulation of the ALDH1A1, P-gp and BCRP proteins. DEAB treatment led to downregulation of the P-gp protein and BCRP transcript and protein. Our results indicate that ALDH1A1-positive cancer cells can be responsible for drug resistance development in ovarian cancer. Developing more specific ALDH1A1 inhibitors can increase chemotherapy effectiveness in ovarian cancer.

  15. A novel dopamine transporter transgenic mouse line for identification and purification of midbrain dopaminergic neurons reveals midbrain heterogeneity

    DEFF Research Database (Denmark)

    Christiansen, Mia Apuschkin; Stilling, Sara; Rahbek-Clemmensen, Troels;

    2015-01-01

    Midbrain dopaminergic (DAergic) neurons are a heterogeneous cell group, composed of functionally distinct cell populations projecting to the basal ganglia, prefrontal cortex and limbic system. Despite their functional significance, the midbrain population of DAergic neurons is sparse, constituting...... of the dopamine transporter (DAT) promoter was characterized. Confocal microscopy analysis of brain sections showed strong eGFP signal reporter in midbrain regions and striatal terminals that co-localized with the DAergic markers DAT and tyrosine hydroxylase (TH). Thorough quantification of co......-localization of the eGFP reporter signal with DAT and TH in the ventral midbrain showed that a vast majority of eGFP-expressing neurons are DAergic. Importantly, expression profiles also revealed DAergic heterogeneity when comparing substantia nigra and ventral tegmental area. Dat1-eGFP mice showed neither change...

  16. Auxin Transport and Ribosome Biogenesis Mutant/Reporter Lines to Study Plant Cell Growth and Proliferation under Altered Gravity

    Science.gov (United States)

    Valbuena, Miguel A.; Manzano, Ana I.; van Loon, Jack JWA.; Saez-Vasquez, Julio; Carnero-Diaz, Eugenie; Herranz, Raul; Medina, F. J.

    2013-02-01

    We tested different Arabidopsis thaliana strains to check their availability for space use in the International Space Station (ISS). We used mutants and reporter gene strains affecting factors of cell proliferation and cell growth, to check variations induced by an altered gravity vector. Seedlings were grown either in a Random Positioning Machine (RPM), under simulated microgravity (μg), or in a Large Diameter Centrifuge (LDC), under hypergravity (2g). A combination of the two devices (μgRPM+LDC) was also used. Under all gravity alterations, seedling roots were longer than in control 1g conditions, while the levels of the nucleolar protein nucleolin were depleted. Alterations in the pattern of expression of PIN2, an auxin transporter, and of cyclin B1, a cell cycle regulator, were shown. All these alterations are compatible with previous space data, so the use of these strains will be useful in the next experiments in ISS, under real microgravity.

  17. Analyzing the role of AP-1B in polarized sorting from recycling endosomes in epithelial cells.

    Science.gov (United States)

    Fölsch, Heike

    2015-01-01

    Epithelial cells polarize their plasma membrane into apical and basolateral domains where the apical membrane faces the luminal side of an organ and the basolateral membrane is in contact with neighboring cells and the basement membrane. To maintain this polarity, newly synthesized and internalized cargos must be sorted to their correct target domain. Over the last ten years, recycling endosomes have emerged as an important sorting station at which proteins destined for the apical membrane are segregated from those destined for the basolateral membrane. Essential for basolateral sorting from recycling endosomes is the tissue-specific adaptor complex AP-1B. This chapter describes experimental protocols to analyze the AP-1B function in epithelial cells including the analysis of protein sorting in LLC-PK1 cells lines, immunoprecipitation of cargo proteins after chemical crosslinking to AP-1B, and radioactive pulse-chase experiments in MDCK cells depleted of the AP-1B subunit μ1B.

  18. Analysis of large scale tests for AP-600 passive containment cooling system

    International Nuclear Information System (INIS)

    One unique feature of the AP-600 is its passive containment cooling system (PCCS), which is designed to maintain containment pressure below the design limit for 72 hours without action by the reactor operator. During a design-basis accident, i.e., either a loss-of-coolant or a main steam-line break accident, steam escapes and comes in contact with the much cooler containment vessel wall. Heat is transferred to the inside surface of the steel containment wall by convection and condensation of steam and through the containment steel wall by conduction. Heat is then transferred from the outside of the containment surface by heating and evaporation of a thin liquid film that is formed by applying water at the top of the containment vessel dome. Air in the annual space is heated by both convection and injection of steam from the evaporating liquid film. The heated air and vapor rise as a result of natural circulation and exit the shield building through the outlets above the containment shell. All of the analytical models that are developed for and used in the COMMIX-ID code for predicting performance of the PCCS will be described. These models cover governing conservation equations for multicomponents single phase flow, transport equations for the κ-ε two-equation turbulence model, auxiliary equations, liquid-film tracking model for both inside (condensate) and outside (evaporating liquid film) surfaces of the containment vessel wall, thermal coupling between flow domains inside and outside the containment vessel, and heat and mass transfer models. Various key parameters of the COMMIX-ID results and corresponding AP-600 PCCS experimental data are compared and the agreement is good. Significant findings from this study are summarized

  19. The Role of Monocarboxylate Transporters and Their Chaperone CD147 in Lactate Efflux Inhibition and the Anticancer Effects of Terminalia chebula in Neuroblastoma Cell Line N2-A

    Science.gov (United States)

    Messeha, S. S.; Zarmouh, N. O.; Taka, E.; Gendy, S. G.; Shokry, G. R.; Kolta, M. G.; Soliman, K. F. A.

    2016-01-01

    Aims In the presence of oxygen, most of the synthesized pyruvate during glycolysis in the cancer cell of solid tumors is released away from the mitochondria to form lactate (Warburg Effect). To maintain cell homeostasis, lactate is transported across the cell membrane by monocarboxylate transporters (MCTs). The major aim of the current investigation is to identify novel compounds that inhibit lactate efflux that may lead to identifying effective targets for cancer treatment. Study Design In this study, 900 ethanol plant extracts were screened for their lactate efflux inhibition using neuroblastoma (N2-A) cell line. Additionally, we investigated the mechanism of inhibition for the most potent plant extract regarding monocarboxylate transporters expression, and consequences effects on viability, growth, and apoptosis. Methodology The potency of lactate efflux inhibition of ethanol plant extracts was evaluated in N2-A cells by measuring extracellular lactate levels. Caspase 3- activity and acridine orange/ethidium bromide staining were performed to assess the apoptotic effect. The antiproliferative effect was measured using WST assay. Western blotting was performed to quantify protein expression of MCTs and their chaperone CD147 in treated cells lysates. Results Terminalia chebula plant extract was the most potent lactate efflux inhibitor in N2-A cells among the 900 - tested plant extracts. The results obtained show that extract of Terminalia chebula fruits (TCE) significantly (P = 0.05) reduced the expression of the MCT1, MCT3, MCT4 and the chaperone CD147. The plant extract was more potent (IC50 of 3.59 ± 0.26 μg/ml) than the MCT standard inhibitor phloretin (IC50 76.54 ± 3.19 μg/ml). The extract also showed more potency and selective cytotoxicity in cancer cells than DI-TNC1 primary cell line (IC50 7.37 ± 0.28 vs. 17.35 ± 0.19 μg/ml). Moreover, TCE Inhibited N2-A cell growth (IG50 = 5.20 ± 0.30 μg/ml) and induced apoptosis at the 7.5 μg/ml concentration

  20. Over-expression of the PaAP1 gene from sweet cherry (Prunus avium L.) causes early flowering in Arabidopsis thaliana.

    Science.gov (United States)

    Wang, Jing; Zhang, Xiaoming; Yan, Guohua; Zhou, Yu; Zhang, Kaichun

    2013-02-15

    A homologue of SQUAMOSA/APETALA1, designated PaAP1, was isolated from Prunus avium by reverse transcription-PCR (RT-PCR). The full length of PaAP1 cDNA is 753 bp, and it codes for a polypeptide of 250 amino acid residues. Sequence comparison revealed that PaAP1 belongs to the MADS-box gene family. Phylogenetic analysis indicated that PaAP1 shared the highest identity with SQUA/AP1 homologues from Prunus serrulata. Real-time fluorescence quantitative PCR analysis showed that PaAP1 was expressed at high levels in petal, sepal, style, and flower buds, which was slightly different from the expression pattern of AP1 of Arabidopsis thaliana. To characterize the functions of PaAP1, we assessed Arabidopsis transformed with 35S::PaAP1. A total of 8 transgenic T(1) lines with an early flowering phenotype were obtained, and a 3:1 segregation ratio of flowering time was observed in the T(2) generation of 4 lines. This study provides the first functional analysis of an SQUA/AP1 homolog from P. avium and suggests that PaAP1 is potentially useful for shortening the juvenile period in sweet cherry. PMID:23206932

  1. Pentoxifylline Treatment in Acute Pancreatitis (AP)

    Science.gov (United States)

    2016-09-14

    Acute Pancreatitis (AP); Gallstone Pancreatitis; Alcoholic Pancreatitis; Post-ERCP/Post-procedural Pancreatitis; Trauma Acute Pancreatitis; Hypertriglyceridemia Acute Pancreatitis; Idiopathic (Unknown) Acute Pancreatitis; Medication Induced Acute Pancreatitis; Cancer Acute Pancreatitis; Miscellaneous (i.e. Acute on Chronic Pancreatitis)

  2. Knockdown of CDK2AP1 in primary human fibroblasts induces p53 dependent senescence.

    Directory of Open Access Journals (Sweden)

    Khaled N Alsayegh

    Full Text Available Cyclin Dependent Kinase-2 Associated Protein-1 (CDK2AP1 is known to be a tumor suppressor that plays a role in cell cycle regulation by sequestering monomeric CDK2, and targeting it for proteolysis. A reduction of CDK2AP1 expression is considered to be a negative prognostic indicator in patients with oral squamous cell carcinoma and also associated with increased invasion in human gastric cancer tissue. CDK2AP1 overexpression was shown to inhibit growth, reduce invasion and increase apoptosis in prostate cancer cell lines. In this study, we investigated the effect of CDK2AP1 downregulation in primary human dermal fibroblasts. Using a short-hairpin RNA to reduce its expression, we found that knockdown of CDK2AP1 in primary human fibroblasts resulted in reduced proliferation and in the induction of senescence associated beta-galactosidase activity. CDK2AP1 knockdown also resulted in a significant reduction in the percentage of cells in the S phase and an accumulation of cells in the G1 phase of the cell cycle. Immunocytochemical analysis also revealed that the CDK2AP1 knockdown significantly increased the percentage of cells that exhibited γ-H2AX foci, which could indicate presence of DNA damage. CDK2AP1 knockdown also resulted in increased mRNA levels of p53, p21, BAX and PUMA and p53 protein levels. In primary human fibroblasts in which p53 and CDK2AP1 were simultaneously downregulated, there was: (a no increase in senescence associated beta-galactosidase activity, (b decrease in the number of cells in the G1-phase and increase in number of cells in the S-phase of the cell cycle, and (c decrease in the mRNA levels of p21, BAX and PUMA when compared with CDK2AP1 knockdown only fibroblasts. Taken together, this suggests that the observed phenotype is p53 dependent. We also observed a prominent increase in the levels of ARF protein in the CDK2AP1 knockdown cells, which suggests a possible role of ARF in p53 stabilization following CDK2AP1

  3. Tank 241-AP-106, Grab samples, 6AP-98-1, 6AP-98-2 and 6AP-98-3 Analytical results for the final report

    Energy Technology Data Exchange (ETDEWEB)

    FULLER, R.K.

    1999-02-23

    This document is the final report for tank 241-AP-106 grab samples. Three grab samples 6AP-98-1, 6AP-98-2 and 6AP-98-3 were taken from riser 1 of tank 241-AP-106 on May 28, 1998 and received by the 222-S Laboratory on May 28, 1998. Analyses were performed in accordance with the ''Compatability Grab Sampling and Analysis Plan'' (TSAP) (Sasaki, 1998) and the ''Data Quality Objectives for Tank Farms Waste Compatability Program (DQO). The analytical results are presented in the data summary report. No notification limits were exceeded. The request for sample analysis received for AP-106 indicated that the samples were polychlorinated biphenyl (PCB) suspects. The results of this analysis indicated that no PCBs were present at the Toxic Substance Control Act (TSCA) regulated limit of 50 ppm. The results and raw data for the PCB analysis are included in this document.

  4. The AP-3 adaptor complex is required for vacuolar function in Arabidopsis

    Institute of Scientific and Technical Information of China (English)

    Maria Zwiewka; Elena Feraru; Barbara M(o)ller; Inhwan Hwang; Mugurel I Feraru; Jürgen Kleine-Vehn; Dolf Weijers; Ji(n) Friml

    2011-01-01

    Subcellular trafficking is required for a multitude of functions in eukaryotic cells.It involves regulation of cargo sorting,vesicle formation,trafficking and fusion processes at multiple levels.Adaptor protein (AP) complexes are key regulators of cargo sorting into vesicles in yeast and mammals but their existence and function in plants have not been demonstrated.Here we report the identification of the protein-affected trafficking 4 (pat4) mutant defective in the putative δ subunit of the AP-3 complex.pat4 and pat2,a mutant isolated from the same GFP imaging-based forward genetic screen that lacks a functional putative AP-3 β,as well as dominant negative AP-3 μ transgenic lines display undistinguishable phenotypes characterized by largely normal morphology and development,but strong intracellular accumulation of membrane proteins in aberrant vacuolar structures.All mutants are defective in morphology and function of lytic and protein storage vacuoles (PSVs) but show normal sorting of reserve proteins to PSVs.Immunoprecipitation experiments and genetic studies revealed tight functional and physical associations of putative AP-3 β and AP-3 δ subunits.Furthermore,both proteins are closely linked with putative AP-3 μ and σ subunits and several components of the clathrin and dynamin machineries.Taken together,these results demonstrate that AP complexes,similar to those in other eukaryotes,exist in plants,and that AP-3 plays a specific role in the regulation of biogenesis and function of vacuoles in plant cells.

  5. Loss of AP-5 results in accumulation of aberrant endolysosomes: defining a new type of lysosomal storage disease.

    Science.gov (United States)

    Hirst, Jennifer; Edgar, James R; Esteves, Typhaine; Darios, Frédéric; Madeo, Marianna; Chang, Jaerak; Roda, Ricardo H; Dürr, Alexandra; Anheim, Mathieu; Gellera, Cinzia; Li, Jun; Züchner, Stephan; Mariotti, Caterina; Stevanin, Giovanni; Blackstone, Craig; Kruer, Michael C; Robinson, Margaret S

    2015-09-01

    Adaptor proteins (AP 1-5) are heterotetrameric complexes that facilitate specialized cargo sorting in vesicular-mediated trafficking. Mutations in AP5Z1, encoding a subunit of the AP-5 complex, have been reported to cause hereditary spastic paraplegia (HSP), although their impact at the cellular level has not been assessed. Here we characterize three independent fibroblast lines derived from skin biopsies of patients harbouring nonsense mutations in AP5Z1 and presenting with spastic paraplegia accompanied by neuropathy, parkinsonism and/or cognitive impairment. In all three patient-derived lines, we show that there is complete loss of AP-5 ζ protein and a reduction in the associated AP-5 µ5 protein. Using ultrastructural analysis, we show that these patient-derived lines consistently exhibit abundant multilamellar structures that are positive for markers of endolysosomes and are filled with aberrant storage material organized as exaggerated multilamellar whorls, striated belts and 'fingerprint bodies'. This phenotype can be replicated in a HeLa cell culture model by siRNA knockdown of AP-5 ζ. The cellular phenotype bears striking resemblance to features described in a number of lysosomal storage diseases (LSDs). Collectively, these findings reveal an emerging picture of the role of AP-5 in endosomal and lysosomal homeostasis, illuminates a potential pathomechanism that is relevant to the role of AP-5 in neurons and expands the understanding of recessive HSPs. Moreover, the resulting accumulation of storage material in endolysosomes leads us to propose that AP-5 deficiency represents a new type of LSDs.

  6. High energy beam lines

    Science.gov (United States)

    Marchetto, M.; Laxdal, R. E.

    2014-01-01

    The ISAC post accelerator comprises an RFQ, DTL and SC-linac. The high energy beam lines connect the linear accelerators as well as deliver the accelerated beams to two different experimental areas. The medium energy beam transport (MEBT) line connects the RFQ to the DTL. The high energy beam transport (HEBT) line connects the DTL to the ISAC-I experimental stations (DRAGON, TUDA-I, GPS). The DTL to superconducting beam (DSB) transport line connects the ISAC-I and ISAC-II linacs. The superconducting energy beam transport (SEBT) line connects the SC linac to the ISAC-II experimental station (TUDA-II, HERACLES, TIGRESS, EMMA and GPS). All these lines have the function of transporting and matching the beams to the downstream sections by manipulating the transverse and longitudinal phase space. They also contain diagnostic devices to measure the beam properties.

  7. APS storage ring vacuum system

    International Nuclear Information System (INIS)

    The Advanced Photon Source synchrotron radiation facility, under construction at the Argonne National Laboratory, incorporates a large ring for the storage of 7 GeV positrons for the generation of photon beams for the facility's experimental program. The Storage Ring's 1104 m circumference is divided into 40 functional sectors. The sectors include vacuum, beam transport, control, acceleration and insertion device components. The vacuum system, which is designed to operate at a pressure of 1 n Torr, consists of 240 connected sections, the majority of which are fabricated from an aluminum alloy extrusion. The sections are equipped with distributed NeG pumping, photon absorbers with lumped pumping, beam position monitors, vacuum diagnostics and valving. The details of the vacuum system design, selected results of the development program and general construction plans are presented. 11 refs., 6 figs., 3 tabs

  8. A high-order unstaggered constrained transport method for the 3D ideal magnetohydrodynamic equations based on the method of lines

    CERN Document Server

    Helzel, Christiane; Taetz, Bertram

    2012-01-01

    Numerical methods for solving the ideal magnetohydrodynamic (MHD) equations in more than one space dimension must confront the challenge of controlling errors in the discrete divergence of the magnetic field. One approach that has been shown successful in stabilizing MHD calculations are constrained transport (CT) schemes. CT schemes can be viewed as predictor-corrector methods for updating the magnetic field, where a magnetic field value is first predicted by a method that does not exactly preserve the divergence-free condition on the magnetic field, followed by a correction step that aims to control these divergence errors. In Helzel et al. (2011) the authors presented an unstaggered constrained transport method for the MHD equations on 3D Cartesian grids. In this work we generalize the method of Helzel et al. (2011) in three important ways: (1) we remove the need for operator splitting by switching to an appropriate method of lines discretization and coupling this with a non-conservative finite volume meth...

  9. Transport of Glial Cell Line-Derived Neurotrophic Factor into Liposomes across the Blood-Brain Barrier: In Vitro and in Vivo Studies

    Directory of Open Access Journals (Sweden)

    Shaoling Wu

    2014-02-01

    Full Text Available Glial cell line-derived neurotrophic factor (GDNF was encapsulated into liposomes in order to protect it from enzyme degradation in vivo and promote its permeability across the blood-brain barrier (BBB. In this study, GDNF conventional liposomes (GDNF-L and GDNF target sterically stabilized liposomes (GDNF-SSL-T were prepared. The average size of liposomes was below 90 nm. A primary model of BBB was established and evaluated by transendothelial electrical resistance (TEER and permeability. This BBB model was employed to study the permeability of GDNF liposomes in vitro. The results indicated that the liposomes could enhance transport of GDNF across the BBB and GDNF-SSL-T had achieved the best transport efficacy. The distribution of GDNF liposomes was studied in vivo. Free GDNF and GDNF-L were eliminated rapidly in the circulation. GDNF-SSL-T has a prolonged circulation time in the blood and favorable brain delivery. The values of the area under the curve (AUC(0–1 h in the brain of GDNF-SSL-T was 8.1 times and 6.8 times more than that of free GDNF and GDNF-L, respectively. These results showed that GDNF-SSL-T realized the aim of targeted delivery of therapeutic proteins to central nervous system.

  10. Transport of glial cell line-derived neurotrophic factor into liposomes across the blood-brain barrier: in vitro and in vivo studies.

    Science.gov (United States)

    Wu, Shaoling; Li, Guoqi; Li, Xiao; Lin, Caina; Yu, Ding; Luan, Shuo; Ma, Chao

    2014-02-27

    Glial cell line-derived neurotrophic factor (GDNF) was encapsulated into liposomes in order to protect it from enzyme degradation in vivo and promote its permeability across the blood-brain barrier (BBB). In this study, GDNF conventional liposomes (GDNF-L) and GDNF target sterically stabilized liposomes (GDNF-SSL-T) were prepared. The average size of liposomes was below 90 nm. A primary model of BBB was established and evaluated by transendothelial electrical resistance (TEER) and permeability. This BBB model was employed to study the permeability of GDNF liposomes in vitro. The results indicated that the liposomes could enhance transport of GDNF across the BBB and GDNF-SSL-T had achieved the best transport efficacy. The distribution of GDNF liposomes was studied in vivo. Free GDNF and GDNF-L were eliminated rapidly in the circulation. GDNF-SSL-T has a prolonged circulation time in the blood and favorable brain delivery. The values of the area under the curve (AUC(0-1 h)) in the brain of GDNF-SSL-T was 8.1 times and 6.8 times more than that of free GDNF and GDNF-L, respectively. These results showed that GDNF-SSL-T realized the aim of targeted delivery of therapeutic proteins to central nervous system.

  11. Regulation of Aggression by Obesity-Linked Genes TfAP-2 and Twz Through Octopamine Signaling in Drosophila

    Science.gov (United States)

    Williams, Michael J.; Goergen, Philip; Rajendran, Jayasimman; Klockars, Anica; Kasagiannis, Anna; Fredriksson, Robert; Schiöth, Helgi B.

    2014-01-01

    In Drosophila, the monoamine octopamine, through mechanisms that are not completely understood, regulates both aggression and mating behavior. Interestingly, our study demonstrates that the Drosophila obesity-linked homologs Transcription factor AP-2 (TfAP-2; TFAP2B in humans) and Tiwaz (Twz; KCTD15 in humans) interact to modify male behavior by controlling the expression of Tyramine β-hydroxylase and Vesicular monanime transporter, genes necessary for octopamine production and secretion. Furthermore, we reveal that octopamine in turn regulates aggression through the Drosophila cholecystokinin satiation hormone homolog Drosulfakinin (Dsk). Finally, we establish that TfAP-2 is expressed in octopaminergic neurons known to control aggressive behavior and that TfAP-2 requires functional Twz for its activity. We conclude that genetically manipulating the obesity-linked homologs TfAP-2 and Twz is sufficient to affect octopamine signaling, which in turn modulates Drosophila male behavior through the regulation of the satiation hormone Dsk. PMID:24142897

  12. Multiplicity of rapidly oscillating Ap stars

    CERN Document Server

    Schoeller, M; Hubrig, S; Kurtz, D W

    2012-01-01

    Rapidly oscillating Ap (roAp) stars have rarely been found in binary or higher order multiple systems. This might have implications for their origin. We intend to study the multiplicity of this type of chemically peculiar stars, looking for visual companions in the range of angular separation between 0.05" and 8". We carried out a survey of 28 roAp stars using diffraction-limited near-infrared imaging with NAOS-CONICA at the VLT. Additionally, we observed three non-oscillating magnetic Ap stars. We detected a total of six companion candidates with low chance projection probabilities. Four of these are new detections, the other two are confirmations. An additional 39 companion candidates are very likely chance projections. We also found one binary system among the non-oscillating magnetic Ap stars. The detected companion candidates have apparent K magnitudes between 6.8 and 19.5 and angular separations ranging from 0.23" to 8.9", corresponding to linear projected separations of 30-2400AU. While our study confi...

  13. Tank 241-AP-107, grab samples 7AP-97-1, 7AP-97-2 and 7AP-97-3 analytical results for the final report

    Energy Technology Data Exchange (ETDEWEB)

    Steen, F.H.

    1997-12-22

    This document is the final report for tank 241-AP-107 grab samples. Three grab samples were collected from riser 1 on September 11, 1997. Analyses were performed on samples 7AP-97-1, 7AP-97-2 and 7AP-97-3 in accordance with the Compatibility Grab Sampling and Analysis Plan (TSAP) (Sasaki, 1997) and the Data Quality Objectives for Tank Farms Waste Compatibility Program (DQO) (Rev. 1: Fowler, 1995; Rev. 2: Mulkey and Nuier, 1997). The analytical results are presented in the data summary report (Table 1). A notification was made to East Tank Farms Operations concerning low hydroxide in the tank and a hydroxide (caustic) demand analysis was requested. The request for sample analysis (RSA) (Attachment 2) received for AP-107 indicated that the samples were polychlorinated biphenyl (PCB) suspects. Therefore, prior to performing the requested analyses, aliquots were made to perform PCB analysis in accordance with the 222-S Laboratory administrative procedure, LAP-101-100. The results of this analysis indicated that no PCBs were present at 50 ppm and analysis proceeded as non-PCB samples. The results and raw data for the PCB analysis will be included in a revision to this document. The sample breakdown diagrams (Attachment 1) are provided as a cross-reference for relating the tank farm customer identification numbers with the 222-S Laboratory sample numbers and the portion of sample analyzed.

  14. Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast.

    Science.gov (United States)

    Ma, Yan; Takeuchi, Mai; Sugiura, Reiko; Sio, Susie O; Kuno, Takayoshi

    2009-08-01

    Adaptins are subunits of the heterotetrameric (beta/mu/gamma/sigma) adaptor protein (AP) complexes that are involved in clathrin-mediated membrane trafficking. Here, we show that in Schizosaccharomyces pombe the deletion strains of each individual subunit of the AP-1 complex [Apl2 (beta), Apl4 (gamma), Apm1 (mu) and Aps1 (sigma)] caused distinct phenotypes on growth sensitivity to temperature or drugs. We also show that the Deltaapm1 and Deltaapl2 mutants displayed similar but more severe phenotypes than those of Deltaaps1 or Deltaapl4 mutants. Furthermore, the Deltaapl2Deltaaps1 and Deltaapl2Deltaapl4 double mutants displayed synthetic growth defects, whereas the Deltaaps1Deltaapl4 and Deltaapl2Deltaapm1 double mutants did not. In pull-down assay, Apm1 binds Apl2 even in the absence of Aps1 and Apl4, and Apl4 binds Aps1 even in the absence of Apm1 and Apl2. Consistently, the deletion of any subunit generally caused the disassociation of the heterotetrameric complex from endosomes, although some subunits weakly localized to endosomes. In addition, the deletion of individual subunits caused similar endosomal accumulation of v-SNARE synaptobrevin Syb1. Altogether, results suggest that the four subunits are all essential for the heterotetrameric complex formation and for the AP-1 function in exit transport from endosomes. PMID:19624755

  15. Present status of design, installation and testing of electron gun and low energy beam transport line of electron Linac at VECC

    International Nuclear Information System (INIS)

    A key component of ANURIB (Advanced National Facility of Unstable and Rare Isotope Beams) project at VECC is a 2 mA, 30/50 MeV continuous-wave superconducting electron linear accelerator (e-Linac). The e-Linac has two sections - a 10 MeV Injector and an Accelerator section for further accelerating the beam to 30/50 MeV. The Injector comprises a 300 kV de thermionic electron gun with gridded cathode modulated at 650 MHz, low energy beam transport (LEBT) line and an injector cryo-module (ICM) that houses one 9-cell beta=1, 1.3 GHz niobium elliptical cavity. As an alternative to the 300 kV gun, a capture cryo-module (CCM) having two single-cell beta=1, 1.3 GHz niobium cavities that will allow the electron gun to be operated at 100 kV is also being developed. The CCM will pre-accelerate the beam from the gun prior to injection in the ICM. The e-Linac has been jointly designed with TRIUMF Canada. The ICM is being built by TRIUMF whereas the front-end of the injector is being built indigenously at VECC. Till the Rajarhat site for ANURIB is getting ready, an e-Linac test area is being setup at VECC Salt Lake campus. The Injector is being installed here and will be later moved to the new campus. The electron gun and several components of the LEBT line up to the CCM have been installed. Alignment and vacuum tests have also been completed. The LEBT line consists of steering magnets, solenoid magnets, diagnostics chamber and a room temperature 1.3 GHz buncher. Solenoid magnets are under fabrication and steering magnets have been procured. A dipole magnet and rf- deflector cavity for characterization of time structure of the beam has been designed and will be added to the LEBT line. In this report, the detail design of the various components and magnets along with present status of installation of the Injector will be presented. (author)

  16. Center for Geometrisk Metrologi CGM ApS, Årsberetning 2001 til DANAK

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2001. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, Erhvervsfremme Styrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler (Teknisk Forskrift Nr. TF4 af 2000.11.0...

  17. Building an AP Social Studies Program with Non-Traditional AP Students

    Science.gov (United States)

    Ashmead, Amanda; Blanchette, Sue

    2013-01-01

    Equal access to education, that is to a high quality education, has increasingly come to mean access to an Advanced Placement program. In recent years, there has been steady attention paid to opening access to AP programs. The 9th annual College Board report (2013) stated "students who succeed on an AP Exam during high school typically…

  18. Chlamydia trachomatis CT771 (nudH) is an asymmetric Ap4A hydrolase

    Science.gov (United States)

    Barta, Michael L.; Lovell, Scott; Sinclair, Amy N.; Battaile, Kevin P.; Hefty, P. Scott

    2014-01-01

    Asymmetric diadenosine 5′,5′″-P1,P4-tetraphosphate (Ap4A) hydrolases are members of the Nudix superfamily that asymmetrically cleave the metabolite Ap4A into ATP and AMP while facilitating homeostasis. The obligate intracellular mammalian pathogen Chlamydia trachomatis possesses a single Nudix family protein, CT771. As pathogens that rely on a host for replication and dissemination typically have one or zero Nudix family proteins, this suggests that CT771 could be critical for chlamydial biology and pathogenesis. We identified orthologs to CT771 within environmental Chlamydiales that share active site residues suggesting a common function. Crystal structures of both apo- and ligand-bound CT771 were determined to 2.6 Å and 1.9 Å resolution, respectively. The structure of CT771 shows a αβα-sandwich motif with many conserved elements lining the putative Nudix active site. Numerous aspects of the ligand-bound CT771 structure mirror those observed in the ligand-bound structure of the Ap4A hydrolase from Caenorhabditis elegans. These structures represent only the second Ap4A hydrolase enzyme member determined from eubacteria and suggest that mammalian and bacterial Ap4A hydrolases might be more similar than previously thought. The aforementioned structural similarities, in tandem with molecular docking, guided the enzymatic characterization of CT771. Together, these studies provide the molecular details for substrate binding and specificity, supporting the analysis that CT771 is an Ap4A hydrolase (nudH). PMID:24354275

  19. A note on weighted Ap(G)-modules

    OpenAIRE

    ÖZTOP, Serap

    2011-01-01

    Let G be a locally compact abelian group and w be a weight function on G. In this paper, we show that the space Ap,w(G) is a Banach module over the Figà-Talamanca Herz algebra Ap(G) and study the multiplier space from Ap(G) to Ap,w(G).

  20. 光动力作用对人结肠癌细胞转录因子激活蛋白-4表达的影响%Inhibition of AP-4 gene expression by zinc phthalocyanine-mediated photodynamic therapy in human colon cancer cell line SW480

    Institute of Scientific and Technical Information of China (English)

    张通; 曹杰; 杨平; 李旺林; 孙政; 张伟健; 曾山崎

    2013-01-01

    目的 观察不同浓度光敏剂酞菁锌对结肠癌细胞株SW480的生长抑制作用及其对转录因子激活蛋白-4(AP-4)表达的影响.方法 应用CCK-8方法 评估光动力作用后SW480细胞的存活率,通过流式细胞技术、RT-PCR技术、Western blot技术检测光敏剂酞菁锌光动力作用后对SW480细胞凋亡和AP-4基因表达等生物学行为的影响.结果 酞菁锌光动力治疗对结肠癌细胞株SW480生长增殖具有明显抑制作用,其效应呈浓度和光照剂量依赖性;流式细胞仪分析显示SW480细胞呈G2/M期阻滞;SW480细胞的凋亡率随酞菁锌浓度增加逐步上升.2.0 μg/mL酞菁锌光动力作用SW480细胞48 h后其AP-4 mRNA水平下降了81%,培养液上清液AP-4蛋白浓度下降了75.6%(P<0.01).结论 应用光敏剂酞菁锌光动力作用能有效抑制SW-480细胞 AP-4的表达,进而抑制细胞的生长、增殖及诱导细胞的凋亡,为结肠癌治疗提供了新的思路和手段.%Objective To investigate the influence of zinc phthalocyanine on the proliferation and AP - 4 gene expression in SW480 cells in vitro. Methods The cell proliferation was analyzed by cell counting kit - 8 ( CCK8 ) assay, while the AP - 4 mRNA and protein expression after photodynamic therapy ( PDT ) were analyzed by RT - PCR and Western blot, respectively. Meanwhile, the flow cytometry ( FCM ) was used to detect the cell apoptosis and cycle. Results The proliferation of SW480 cells was significantly suppressed by zinc phthalocyanine - mediated PDT in concentration - and light dose - dependent manners. The cell was arrested at G2/M by PDT, while the apoptosis rate was enhanced with zinc phthalocyanine in concentration dependent manner. After 2. 0 μg/mL zinc phthalocyanine - mediated PDT, the AP - 4 mRNA and protein were significantly reduced by 81% and 75. 6% , respectively, in SW480 cells ( P <0. 01 ). Conclusion Zinc phthalocyanine - mediated PDT can effectively suppress the expression of AP - 4, and

  1. AP1000, a nuclear central of advanced design; AP1000, una central nuclear de diseno avanzado

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez M, N.; Viais J, J. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)]. e-mail: nhm@nuclear.inin.mx

    2005-07-01

    The AP1000 is a design of a nuclear reactor of pressurized water (PWR) of 1000 M We with characteristic of safety in a passive way; besides presenting simplifications in the systems of the plant, the construction, the maintenance and the safety, the AP1000 is a design that uses technology endorsed by those but of 30 years of operational experience of the PWR reactors. The program AP1000 of Westinghouse is focused to the implementation of the plant to provide improvements in the economy of the same one and it is a design that is derived directly of the AP600 designs. On September 13, 2004 the US-NRC (for their initials in United States- Nuclear Regulatory Commission) approved the final design of the AP1000, now Westinghouse and the US-NRC are working on the whole in a complete program for the certification. (Author)

  2. An analysis of AP600 design features

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jong Kyoon; Jang, Moon Heui; Hwang, Yung Dong [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)] [and others

    1994-01-01

    In the aspect of engineering, passive safety system concept has improved the safety degree of nuclear power plant. Therefore, the objective of this study is to check on the possibility of the capacity upgrade of nuclear power plant in the case of adopting the passive safety system concept of AP 600. The characteristics of AP 600 are the advanced functions in ECCS, heat removal of containment building and residual heat removal under the passive safety system concept. The result of this study will become the basic data of capacity upgrade of nuclear power plant and will be widely used in second year project. (Author).

  3. Formalistic approaches to A&P

    Institute of Scientific and Technical Information of China (English)

    赵倩

    2014-01-01

    A&P is a famous short story. Its simple words, the unique point of view and skillful writing technique are expressed successfully. Besides, the first-person narrator is also a good point. From Sammy’s eyes, readers can learn the character’s characteristics. Another point is the usage of symbol, the name of the store, A&P, it shows that people’s life is rich, but their spirit is barren. This article tries to analyze it by formalistic approaches.

  4. Project W-519 CDR supplement: Raw water and electrical services for privatization contractor, AP tank farm operations

    Energy Technology Data Exchange (ETDEWEB)

    Parazin, R.J.

    1998-07-31

    This supplement to the Project W-519 Conceptual Design will identify a means to provide RW and Electrical services to serve the needs of the TWRS Privatization Contractor (PC) at AP Tank Farm as directed by DOE-RL. The RW will serve the fire suppression and untreated process water requirements for the PC. The purpose of this CDR supplement is to identify Raw Water (RW) and Electrical service line routes to the TWRS Privatization Contractor (PC) feed delivery tanks, AP-106 and/or AP-108, and establish associated cost impacts to the Project W-519 baseline.

  5. Butyrate Produced by Commensal Bacteria Potentiates Phorbol Esters Induced AP-1 Response in Human Intestinal Epithelial Cells

    OpenAIRE

    Nepelska, Malgorzata; Cultrone, Antonietta; Béguet-Crespel, Fabienne; Le Roux, Karine; Doré, Joël; Arulampalam, Vermulugesan; Blottière, Hervé M.

    2012-01-01

    The human intestine is a balanced ecosystem well suited for bacterial survival, colonization and growth, which has evolved to be beneficial both for the host and the commensal bacteria. Here, we investigated the effect of bacterial metabolites produced by commensal bacteria on AP-1 signaling pathway, which has a plethora of effects on host physiology. Using intestinal epithelial cell lines, HT-29 and Caco-2, stably transfected with AP-1-dependent luciferase reporter gene, we tested the effect...

  6. Kepler observations of rapidly oscillating Ap, δ Scuti and γ Doradus pulsations in Ap stars

    DEFF Research Database (Denmark)

    Balona, Luis A.; Cunha, Margarida S.; Kurtz, Donald W.;

    2011-01-01

    Observations of the A5p star KIC 8677585 obtained during the Kepler 10-d commissioning run with 1-min time resolution show that it is a rapidly oscillating Ap (roAp) star with several frequencies with periods near 10 min. In addition, a low frequency at 3.142 d−1 is also clearly present. Multiper......Observations of the A5p star KIC 8677585 obtained during the Kepler 10-d commissioning run with 1-min time resolution show that it is a rapidly oscillating Ap (roAp) star with several frequencies with periods near 10 min. In addition, a low frequency at 3.142 d−1 is also clearly present....... Multiperiodic γ Doradus (γ Dor) and δ Scuti (δ Sct) pulsations, never before seen in any Ap star, are present in Kepler observations of at least three other Ap stars. Since γ Dor pulsations are seen in Ap stars, it is likely that the low frequency in KIC 8677585 is also a γ Dor pulsation. The simultaneous...

  7. Stokes IQUV Magnetic Doppler Imaging of Ap stars I. ESPaDOnS and NARVAL Observations

    OpenAIRE

    Silvester, J.; Wade, G. A.; Kochukhov, O.; Bagnulo, S.; Folsom, C. P.; Hanes, D.

    2012-01-01

    In this paper we describe and evaluate new spectral line polarisation observations obtained with the goal of mapping the surfaces of magnetic Ap stars in great detail. One hundred complete or partial Stokes IQUV sequences, corresponding to 297 individual polarised spectra, have been obtained for 7 bright Ap stars using the ESPaDOnS and NARVAL spectropolarimeters. The targets span a range of mass from approximately 1.8 to 3.4 solar mass, a range of rotation period from 2.56 to 6.80 days, and a...

  8. Two Successful Approaches to Teaching AP Government

    Science.gov (United States)

    Ladd, Brian; Stepp, Heidi

    2013-01-01

    Amador Valley High School, in Pleasanton, California, uses two unique approaches to teaching Advanced Placement Government and Politics. AP Government consists of six units: Constitutional Underpinnings; Political Behavior and Political Beliefs; Mass Media, Interest Groups, and Political Parties; Institutions of Government; Civil Liberties and…

  9. 2000 Johnston Site 3A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 3A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on July 3, 2000. With a start point (meter 0) at...

  10. 2000 Johnston Site 2A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 2A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on June 30, 2000. With a start point (meter 0) at...

  11. The Demographic Wave: Rethinking Hispanic AP Trends

    Science.gov (United States)

    Edwards, Kelcey; Sawtell, Ellen

    2013-01-01

    Presented at the Advanced Placement Annual Conference (APAC) in Las Vegas, NV in July 2013. This presentation reviews new research examining the AP® experience of Hispanic graduates over the past decade. Topics include an in-depth look at the AP Spanish Language and Culture gateway hypothesis and trends in family characteristics such as parent…

  12. 2006 Johnston Site 1A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 1A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on June 29, 2000. With a start point (meter 0) at...

  13. 2000 Johnston Site 1A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 1A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on June 29, 2000. With a start point (meter 0) at...

  14. Structuring the AP Art History Course

    Science.gov (United States)

    Herscher, Walter R.

    2013-01-01

    While AP (Advanced Placement) Art History may be taught within the art department in many schools, social studies teachers are equally capable of teaching the course well. They have the historical background to discuss the reasons for changes in art styles. A teacher's preparation is similar to teaching a course stressing political history,…

  15. Review on Transport Organization for the Disassemble Project of Guangzhou Metro Line 2%广州地铁原2号线拆解后的车务组织回顾

    Institute of Scientific and Technical Information of China (English)

    宋利明

    2011-01-01

    The original Guangzhou metro Line 2 started operation in June, 2003. In September, 2010, this line was disassembled and linked with a new part (with 20 stations and 26.7 km in length) to form new Line 2 and Line 8. Only 3 days were given to finish the disassemble project, and the new lines would be opened for operation right after the project. Since the original Line 2 had a large passenger flow,so the new one is required to maintain the same transportation capacity after disassemble work. However, there isn't any pre-operation drilling for the new lines, it surely requires a well-prepared transport organization before the disassemble project. This paper takes a review on the transport organization for the disassemble project of Guangzhou metro, Line 2.%广州地铁原2号线于2010年9月拆解,连同新开通的26.7 km,20座车站一起,形成新的2号线和8号线.由于原2号线停运工程拆解时间短,拆解后要立即按2条线来运营,故拆解前不具备条件进行运营演练.这就要求车务部门在拆解前做好车务组织工作.介绍了原2号线拆解前的运营车务组织工作,包括新2,8号线开通前,确定设备故障演练项目、进行时刻表演练等,为类似的城市轨道交通新线运营筹备工作提供参考.

  16. Abnormal lithium abundance in several Ap-Bp stars

    CERN Document Server

    Shavrina, A; Drake, N A; Kudryavtsev, D O; Gopka, V F; Yushchenko, V A; Yushchenko, A V

    2013-01-01

    Using observations of rapidly rotating roAp star HD 12098, obtained with 6m telescope of SAO RAS in 2005-2008 in nine rotational phases (0.001-0.817), and code V. Tsymbal ROTATE we identified 3 lithium spots on the surface of star, one of them coincided with the position of one magnetic pole and two others are located near the other pole, symmetrically with respect to it. Perhaps magnetic field of the star has a more complex structure than the dipole (Shavrina 2008). Lithium abundance in the spots is greater than cosmic one. It can be explained by diffusion of lithium in the presence of a magnetic field or by production of lithium in the reactions of "spallation" on the surface of star near the magnetic poles (see, for example, Goriely 2007). Enhanced lithium abundances was also determined for other two slowly rotating Ap stars HR 465 and HD 965. The analysis is performed using a synthetic spectrum method taking into account magnetic splitting of lines by the SYNTHM code of Khan (2004).

  17. Tank 241-AP-106, grab samples, 6AP-96-1 through 6AP-96-3 analytical results for the final report

    Energy Technology Data Exchange (ETDEWEB)

    Esch, R.A., Westinghouse Hanford

    1996-12-11

    This document is the final report for tank 241-AP-106 grab samples. This document presents the analytical results for three samples (6AP-96-1, 6AP-96-2 and 6AP-96-3) taken from riser 1 @ 150{degrees} of tank 241-AP-1 06 on September 12, 1996. Analyses were performed in accordance with the Compatibility Grab Sampling and Analysis Plan (TSAP) (Sasaki, 1996) and the Data Quality Objectives for Tank Farms Waste Compatibility Program (DQO) (Fowler, 1995).

  18. The Clathrin Adaptor AP-1A Mediates Basolateral Polarity

    OpenAIRE

    Gravotta, Diego; Carvajal-Gonzalez, Jose Maria; Mattera, Rafael; Deborde, Sylvie; Banfelder, Jason R.; Bonifacino, Juan S.; Rodriguez-Boulan, Enrique

    2012-01-01

    Clathrin and the epithelial-specific clathrin adaptor AP-1B mediate basolateral trafficking in epithelia. However, several epithelia lack AP-1B and mice knocked-out for AP-1B are viable, suggesting the existence of additional mechanisms that control basolateral polarity. Here, we demonstrate a distinct role of the ubiquitous clathrin adaptor AP-1A in basolateral protein sorting. Knock-down of AP-1A causes missorting of basolateral proteins in MDCK cells but only after knock-down of AP-1B, sug...

  19. Enhanced NFκB and AP-1 transcriptional activity associated with antiestrogen resistant breast cancer

    International Nuclear Information System (INIS)

    Signaling pathways that converge on two different transcription factor complexes, NFκB and AP-1, have been identified in estrogen receptor (ER)-positive breast cancers resistant to the antiestrogen, tamoxifen. Two cell line models of tamoxifen-resistant ER-positive breast cancer, MCF7/HER2 and BT474, showing increased AP-1 and NFκB DNA-binding and transcriptional activities, were studied to compare tamoxifen effects on NFκB and AP-1 regulated reporter genes relative to tamoxifen-sensitive MCF7 cells. The model cell lines were treated with the IKK inhibitor parthenolide (PA) or the proteasome inhibitor bortezomib (PS341), alone and in combination with tamoxifen. Expression microarray data available from 54 UCSF node-negative ER-positive breast cancer cases with known clinical outcome were used to search for potential genes signifying upregulated NFκB and AP-1 transcriptional activity in association with tamoxifen resistance. The association of these genes with patient outcome was further evaluated using node-negative ER-positive breast cancer cases identified from three other published data sets (Rotterdam, n = 209; Amsterdam, n = 68; Basel, n = 108), each having different patient age and adjuvant tamoxifen treatment characteristics. Doses of parthenolide and bortezomib capable of sensitizing the two endocrine resistant breast cancer models to tamoxifen were capable of suppressing NFκB and AP-1 regulated gene expression in combination with tamoxifen and also increased ER recruitment of the transcriptional co-repressor, NCoR. Transcript profiles from the UCSF breast cancer cases revealed three NFκB and AP-1 upregulated genes – cyclin D1, uPA and VEGF – capable of dichotomizing node-negative ER-positive cases into early and late relapsing subsets despite adjuvant tamoxfien therapy and most prognostic for younger age cases. Across the four independent sets of node-negative ER-positive breast cancer cases (UCSF, Rotterdam, Amsterdam, Basel), high expression of

  20. Enhanced NFκB and AP-1 transcriptional activity associated with antiestrogen resistant breast cancer

    Directory of Open Access Journals (Sweden)

    Moore Dan H

    2007-04-01

    Full Text Available Abstract Background Signaling pathways that converge on two different transcription factor complexes, NFκB and AP-1, have been identified in estrogen receptor (ER-positive breast cancers resistant to the antiestrogen, tamoxifen. Methods Two cell line models of tamoxifen-resistant ER-positive breast cancer, MCF7/HER2 and BT474, showing increased AP-1 and NFκB DNA-binding and transcriptional activities, were studied to compare tamoxifen effects on NFκB and AP-1 regulated reporter genes relative to tamoxifen-sensitive MCF7 cells. The model cell lines were treated with the IKK inhibitor parthenolide (PA or the proteasome inhibitor bortezomib (PS341, alone and in combination with tamoxifen. Expression microarray data available from 54 UCSF node-negative ER-positive breast cancer cases with known clinical outcome were used to search for potential genes signifying upregulated NFκB and AP-1 transcriptional activity in association with tamoxifen resistance. The association of these genes with patient outcome was further evaluated using node-negative ER-positive breast cancer cases identified from three other published data sets (Rotterdam, n = 209; Amsterdam, n = 68; Basel, n = 108, each having different patient age and adjuvant tamoxifen treatment characteristics. Results Doses of parthenolide and bortezomib capable of sensitizing the two endocrine resistant breast cancer models to tamoxifen were capable of suppressing NFκB and AP-1 regulated gene expression in combination with tamoxifen and also increased ER recruitment of the transcriptional co-repressor, NCoR. Transcript profiles from the UCSF breast cancer cases revealed three NFκB and AP-1 upregulated genes – cyclin D1, uPA and VEGF – capable of dichotomizing node-negative ER-positive cases into early and late relapsing subsets despite adjuvant tamoxfien therapy and most prognostic for younger age cases. Across the four independent sets of node-negative ER-positive breast cancer cases

  1. Dynamics of intracellular clathrin/AP1 and clathrin/AP3 containing carriers

    OpenAIRE

    2012-01-01

    Clathrin/AP1 and clathrin/AP3 coated vesicular carriers originate from endosomes and the TGN. We report here the real-time visualization of these structures in living cells reliably tracked by using rapid, three-dimensional imaging with a spinning-disk confocal microscope. We imaged relatively sparse, diffraction-limited, fluorescent objects containing chimeric fluorescent protein (clathrin light chain, σ adaptor subunits or dynamin2) with a spatial precision of up to ~ 30 nm and a temporal r...

  2. Discovery of new roAp pulsators in the UVES survey of cool magnetic Ap stars

    CERN Document Server

    Kochukhov, O; Ryabchikova, T; Boyko, S; Cunha, M; Tsymbal, V; Weiss, W

    2013-01-01

    We have carried out a survey of short-period pulsations among a sample of carefully chosen cool Ap stars using time-resolved observations with the UVES spectrometer at the ESO 8-m VLT telescope. Here we report the discovery of pulsations with amplitudes 50-100 m/s and periods 7-12 min in HD132205, HD148593 and HD151860. These objects are therefore established as new rapidly oscillating Ap (roAp) stars. In addition, we independently confirm the presence of pulsations in HD69013, HD96237 and HD143487 and detect, for the first time, radial velocity oscillations in two previously known photometric roAp stars HD119027 and HD185256. At the same time, no pulsation variability is found for HD5823, HD178892 and HD185204. All of the newly discovered roAp stars were previously classified as non-pulsating based on the low-precision ground-based photometric surveys. This shows that such observations cannot be used to reliably distinguish between pulsating and non-pulsating stars and that all cool Ap stars may harbor p-mod...

  3. Comparative analysis between radiographic views for knee osteoarthrosis (bipedal AP versus monopedal AP

    Directory of Open Access Journals (Sweden)

    Rodrigo Pires e Albuquerque

    2013-08-01

    Full Text Available OBJECTIVE: A comparative analysis by applying the criteria of the original classification Ahlbäck in the anteroposterior (AP bipedal knee in extension and anteroposterior (AP monopodal knee in symptomatic knee arthrosis. With this analysis we intend to observe the agreement, any advantage or difference between the incidence and degree of joint involvement between the orthopedic surgeons and radiologists with the referring physician. METHODS: From January 2012 to March 2012, was a prospective study of 60 symptomatic arthrosis knees (60 patients, clinically selected group of outpatient knee and radiographic proposals submitted to the search. Of the 60 patients, 39 were female and 21 male, mean age 64 years (ranging from 50 to 84 years. Of the 60 knees studied, 37 corresponded to the right side and 23 on the left side. Statistical analysis was performed by Kappa statistics, which evaluates the interobserver agreement for qualitative data. RESULTS: According to the scale of Ahlbäck, there was a significant agreement (p < 0.0001 intra-observer in the classification of knee osteoarthritis among the five evaluators. There was a significant agreement (p < 0.0001 with inter-observer referring physician in the incidence of AP monopodal and AP bipedal for the four raters. CONCLUSION: The study found no difference between the incidence in the AP monopodal versus AP bipedal in osteoarthritis of the knee.

  4. Analysis on the Track Quality Situation of Jiaoji Passenger Transport Line%胶济客运专线轨道质量状态分析

    Institute of Scientific and Technical Information of China (English)

    张紫菱

    2015-01-01

    目前有砟轨道在我国高速铁路中占有重要地位,研究有砟轨道质量状态的发展对高速铁路的养护维修工作具有重要意义。从轨道不平顺指数方面对胶济客运专线的轨道检查数据进行了分析,发现单项指数中高低、轨向、水平和三角坑的影响相对较大,维修周期内轨道TQI指数在上下波动的同时逐渐增大并呈线性增长趋势,TQI指数增加到7左右时安排大机维修作业较为合理,大机维修作业后轨道TQI指数可以降低到4.5左右。%At present, ballast track occupies an important place in the high-speed railway of China. It has important significance to study the quality development of ballast track for the maintenance and repair work of high-speed railway. The track inspection data of Jiaoji passenger transport line is analyzed from the track irregularity index. It finds that the effect of the single index such as discretion, track alignment, horizontal and twist warp is bigger. TQI index of track fluctuates, at the same time it gradually increases and has a linear growth trend in the maintenance cycle. When TQI index increases to about 7, the arrangement of big machine maintenance work is more reasonable. After the big machine maintenance work, TQI index can reduce to about 4.5.

  5. Magnetic Doppler Imaging of Ap stars

    OpenAIRE

    Silvester, J.; Wade, G. A.; Kochukhov, O.; Landstreet, J. D.; Bagnulo, S.

    2007-01-01

    Historically, the magnetic field geometries of the chemically peculiar Ap stars were modelled in the context of a simple dipole field. However, with the acquisition of increasingly sophisticated diagnostic data, it has become clear that the large-scale field topologies exhibit important departures from this simple model. Recently, new high-resolution circular and linear polarisation spectroscopy has even hinted at the presence of strong, small-scale field structures, which were completely une...

  6. APS storage ring vacuum system performance

    International Nuclear Information System (INIS)

    The Advanced Photon Source (APS) storage ring was designed to operated with 7-GeV, 100-mA positron beam with lifetimes > 20 hours. The lifetime is limited by residual gas scattering and Touschek scattering at this time. Photon-stimulated desorption and microwave power in the rf cavities are the main gas loads. Comparison of actual system gas loads and design calculations will be given. In addition, several special features of the storage ring vacuum system will be presented

  7. Cultural Diversity in AP Art History

    Science.gov (United States)

    Bolte, Frances R.

    2006-01-01

    Teaching AP Art History is like running on a treadmill that is moving faster than a teacher can run. Many teachers are out of breath before the end of the term and wonder how in the world they can cover every chapter. Because time is short and art from pre-history through to the present, including the non-European traditions, must be covered, this…

  8. AP-42 ADDITIONS AND REVISIONS - RESIDENTIAL WOOD COMBUSTION

    Science.gov (United States)

    This project develops emission factors, etc., for residential fireplaces and woodstoves which are incorporated into AP-42. AP42 is a massive collection of material which describes processes which generate air emissions and presents emission factors and control effectiveness infor...

  9. Dicty_cDB: FC-AP07 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP07 (Link to dictyBase) - - - Contig-U15932-1 FC-AP07P (Li...nk to Original site) FC-AP07F 546 FC-AP07Z 446 FC-AP07P 992 - - Show FC-AP07 Library FC (Link to library) Clone ID FC-AP...al site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP07Q.Seq.d/ Representative seq. ID FC-AP...07P (Link to Original site) Representative DNA sequence >FC-AP07 (FC-AP07Q) /CSM/FC/FC-AP/FC-AP...ificant alignments: (bits) Value FC-AP07 (FC-AP07Q) /CSM/FC/FC-AP/FC-AP07Q.Seq.d/ 1148 0.0 SSM404 (SSM404Q)

  10. Dicty_cDB: FC-AP08 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP08 (Link to dictyBase) - - - Contig-U16116-1 FC-AP08P (Li...nk to Original site) FC-AP08F 125 FC-AP08Z 352 FC-AP08P 477 - - Show FC-AP08 Library FC (Link to library) Clone ID FC-AP...al site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP08Q.Seq.d/ Representative seq. ID FC-AP...08P (Link to Original site) Representative DNA sequence >FC-AP08 (FC-AP08Q) /CSM/FC/FC-AP/FC-AP... (VSA612Q) /CSM/VS/VSA6-A/VSA612Q.Seq.d/ 624 e-178 FC-AP08 (FC-AP08Q) /CSM/FC/FC-AP/FC-AP

  11. AP Deployment Research Based on Physical Distance and Channel Isolation

    OpenAIRE

    Dangui Yan; Chengchang Zhang; Honghua Liao; Lisheng Yang; Ping Li; Guogang Yang

    2014-01-01

    Aiming at the problem of inefficiency of wireless local area networks (WLAN) access point (AP) deployment in urban environment, a new algorithm for AP deployment based on physical distance and channel isolation (DPDCI) is proposed. First, it detects the position information of deployed APs and then calculates the interference penalty factor combined with physical distance and channel isolation, and finally gets the optimal location and channel of the new AP through the genetic algorithm. Comp...

  12. CYLD Inhibits Tumorigenesis and Metastasis by Blocking JNK/AP1 Signaling at Multiple Levels

    Science.gov (United States)

    de Marval, Paula Miliani; Lutfeali, Shazia; Jin, Jane Y.; Leshin, Benjamin; Selim, M. Angelica; Zhang, Jennifer Y.

    2011-01-01

    CYLD has been recognized as a tumor suppressor due to its dominant genetic linkage to multiple types of epidermal tumors and a range of other cancers. The molecular mechanisms governing CYLD control of skin cancer are still unclear. Here, we demonstrated that K14-driven epidermal expression of a patient relevant and catalytically deficient CYLD truncation mutant (CYLDm) sensitized mice to skin tumor development in response to DMBA/TPA-challenge. Tumors developed on transgenic mice were prone to malignant progression and lymph node metastasis, and displayed increased activation of JNK and the downstream c-Jun and c-Fos proteins. Most importantly, topical application of a pharmacological JNK inhibitor significantly reduced tumor development and abolished metastasis in the transgenic mice. Further in line with these animal data, exogenous expression of CYLDm in A431, a human squamous cell carcinoma (SCC) cell line, markedly enhanced cell growth, migration and subcutaneous tumor growth in an AP1-depdendent manner. In contrast, expression of the wild type CYLD inhibited SCC tumorigenesis and AP1 function. Most importantly, CYLDm not only increased JNK activation but also induced an upregulation of K63-ubiquitination on both c-Jun and c-Fos, leading to sustained AP1 activation. Our findings uncovered c-Jun and c-Fos as novel CYLD-targets and underscore that CYLD controls epidermal tumorigenesis through blocking the JNK/AP1 signaling pathway at multiple levels. PMID:21478324

  13. Pharmakologische Charakterisierung der vasoaktiven Eigenschaften von Ap7A und Ap8A an der isolierten perfundierten Rattenniere

    OpenAIRE

    Blaschke, Daniela

    2010-01-01

    Recently, diadenosine heptaphosphate and diadenosine octaphosphate have been isolated in human platelets. With thrombin-induced platelet aggregation, Ap7A and Ap8A are released from the platelets into the extracellular space. Like other dinucleoside polyphosphates, maybe they play an important role in the control of vascular tone and platelet aggregation. Here we describe the effects on regulation of vascular tone of Ap7A and Ap8A in isolated perfused rat kidney. Our experiments demonstrate t...

  14. Study on modular construction management in AP1000 nuclear plant project

    International Nuclear Information System (INIS)

    The construction of AP1000 Nuclear Power Plant (NPP) has commenced in China. The AP1000 NPP features a passive design concept and modular construction technology. Based on the management of the construction of current AP1000 NNP, this paper describes the effects on Nuclear Island (NI) construction project management resulting from modular construction technology, as well as new construction techniques and methods. This paper puts forward new requirements for construction schedule management of the nuclear island construction at different levels. The AP1000 NI construction logic features the parallel construction of civil and structural erection as the main approach, with the integrated schedule of module fabrication, assembly and installation as support. The structural modules of AP1000 project are prefabricated in shop, delivered to site as sub-modules and assembled to integrated structural module. The assembled module is transported to the construction site, hoisted and finally set in NI. This paper illustrates how to ensure the construction quality of structural modules by analyzing the interface process and key links in the quality control program, and introduces how to ensure the safety of heavy structural components during various construction phases by evaluating and analyzing the construction safety process. This paper also makes an analysis of the safe environment for the assembly and installation of Containment Vessel, the management of product protection and personnel safety inside the Containment Building during 'Open Top' construction, raises to implement effective protection for the numerous pre-set mechanical modules and equipments, as well as personnel safety protection programs and measures. The modular construction feature of AP1000 NPP design requires technique improvement and management innovation during the NI construction. This paper makes a study and research on the control management of schedule, quality and safety of AP1000 NPP NI

  15. Dicty_cDB: FC-AP01 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP01 (Link to dictyBase) - - - Contig-U15092-1 FC-AP01Z (Li...nk to Original site) - - FC-AP01Z 591 - - - - Show FC-AP01 Library FC (Link to library) Clone ID FC-AP01 (Li.../dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP01Q.Seq.d/ Representative seq. ID FC-AP...01Z (Link to Original site) Representative DNA sequence >FC-AP01 (FC-AP01Q) /CSM/FC/FC-AP/FC-AP01Q.Seq....EELNISGPLSRNKLKWADFLNLTMNTNHARG HRHGRSPSKIFWRAVRGMLPHKTPRGQAALDNMKVFEGVPAPYDKVKRVVVPSALRVVKL NTTRKYTVLSRLSQE

  16. Dicty_cDB: FC-AP10 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP10 (Link to dictyBase) - - - Contig-U15819-1 FC-AP10Z (Li...nk to Original site) - - FC-AP10Z 497 - - - - Show FC-AP10 Library FC (Link to library) Clone ID FC-AP10 (Li.../dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP10Q.Seq.d/ Representative seq. ID FC-AP...10Z (Link to Original site) Representative DNA sequence >FC-AP10 (FC-AP10Q) /CSM/FC/FC-AP/FC-AP10Q.Seq....SGDWWDAELKGRRGKVPSNYLQLIKNAAPPRAGGPPVPTGNRA PTTTTTSGGSTRGGFNNGPSTAPSGRGAAPPSSRGGMAPRGGSVAPPSSRGGIAPRGGIA PRGGMAPRGGMAP

  17. MiniAp-4: A Venom-Inspired Peptidomimetic for Brain Delivery.

    Science.gov (United States)

    Oller-Salvia, Benjamí; Sánchez-Navarro, Macarena; Ciudad, Sonia; Guiu, Marc; Arranz-Gibert, Pol; Garcia, Cristina; Gomis, Roger R; Cecchelli, Roméo; García, Jesús; Giralt, Ernest; Teixidó, Meritxell

    2016-01-11

    Drug delivery across the blood-brain barrier (BBB) is a formidable challenge for therapies targeting the central nervous system. Although BBB shuttle peptides enhance transport into the brain non-invasively, their application is partly limited by lability to proteases. The present study proposes the use of cyclic peptides derived from venoms as an affordable way to circumvent this drawback. Apamin, a neurotoxin from bee venom, was minimized by reducing its complexity, toxicity, and immunogenicity, while preserving brain targeting, active transport, and protease resistance. Among the analogues designed, the monocyclic lactam-bridged peptidomimetic MiniAp-4 was the most permeable. This molecule is capable of translocating proteins and nanoparticles in a human-cell-based BBB model. Furthermore, MiniAp-4 can efficiently deliver a cargo across the BBB into the brain parenchyma of mice. PMID:26492861

  18. 77 FR 24480 - Application for New Awards; Advanced Placement (AP) Test Fee Program-Reopening the AP Test Fee...

    Science.gov (United States)

    2012-04-24

    .... ACTION: Notice reopening the AP Test Fee fiscal year 2012 competition. Catalog of Federal Domestic Assistance (CFDA) Number: 84.330B. SUMMARY: On February 15, 2012, we published in the Federal Register (77 FR... Application for New Awards; Advanced Placement (AP) Test Fee Program--Reopening the AP Test Fee Fiscal...

  19. AP1000R nuclear power plant safety overview for spent fuel cooling

    International Nuclear Information System (INIS)

    The AP1000R plant is an 1100-MWe class pressurized water reactor with passive safety features and extensive plant simplifications that enhance construction, operation, maintenance, safety and costs. The AP1000 design uses passive features to mitigate design basis accidents. The passive safety systems are designed to function without safety-grade support systems such as AC power, component cooling water, service water or HVAC. Furthermore, these passive features 'fail safe' during a non-LOCA event such that DC power and instrumentation are not required. The AP1000 also has simple, active, defense-in-depth systems to support normal plant operations. These active systems provide the first level of defense against more probable events and they provide investment protection, reduce the demands on the passive features and support the probabilistic risk assessment. The AP1000 passive safety approach allows the plant to achieve and maintain safe shutdown in case of an accident for 72 hours without operator action, meeting the expectations provided in the U.S. Utility Requirement Document and the European Utility Requirements for passive plants. Limited operator actions are required to maintain safe conditions in the spent fuel pool via passive means. In line with the AP1000 approach to safety described above, the AP1000 plant design features multiple, diverse lines of defense to ensure spent fuel cooling can be maintained for design-basis events and beyond design-basis accidents. During normal and abnormal conditions, defense-in-depth and other systems provide highly reliable spent fuel pool cooling. They rely on off-site AC power or the on-site standby diesel generators. For unlikely design basis events with an extended loss of AC power (i.e., station blackout) or loss of heat sink or both, spent fuel cooling can still be provided indefinitely: - Passive systems, requiring minimal or no operator actions, are sufficient for at least 72 hours under all possible pool heat

  20. AP1000{sup R} nuclear power plant safety overview for spent fuel cooling

    Energy Technology Data Exchange (ETDEWEB)

    Gorgemans, J.; Mulhollem, L.; Glavin, J.; Pfister, A.; Conway, L.; Schulz, T.; Oriani, L.; Cummins, E.; Winters, J. [Westinghouse Electric Company LLC, 1000 Westinghouse Drive, Cranberry Township, PA 16066 (United States)

    2012-07-01

    The AP1000{sup R} plant is an 1100-MWe class pressurized water reactor with passive safety features and extensive plant simplifications that enhance construction, operation, maintenance, safety and costs. The AP1000 design uses passive features to mitigate design basis accidents. The passive safety systems are designed to function without safety-grade support systems such as AC power, component cooling water, service water or HVAC. Furthermore, these passive features 'fail safe' during a non-LOCA event such that DC power and instrumentation are not required. The AP1000 also has simple, active, defense-in-depth systems to support normal plant operations. These active systems provide the first level of defense against more probable events and they provide investment protection, reduce the demands on the passive features and support the probabilistic risk assessment. The AP1000 passive safety approach allows the plant to achieve and maintain safe shutdown in case of an accident for 72 hours without operator action, meeting the expectations provided in the U.S. Utility Requirement Document and the European Utility Requirements for passive plants. Limited operator actions are required to maintain safe conditions in the spent fuel pool via passive means. In line with the AP1000 approach to safety described above, the AP1000 plant design features multiple, diverse lines of defense to ensure spent fuel cooling can be maintained for design-basis events and beyond design-basis accidents. During normal and abnormal conditions, defense-in-depth and other systems provide highly reliable spent fuel pool cooling. They rely on off-site AC power or the on-site standby diesel generators. For unlikely design basis events with an extended loss of AC power (i.e., station blackout) or loss of heat sink or both, spent fuel cooling can still be provided indefinitely: - Passive systems, requiring minimal or no operator actions, are sufficient for at least 72 hours under all

  1. Fgfr3 is a transcriptional target of Ap2delta and Ash2l-containing histone methyltransferase complexes.

    Directory of Open Access Journals (Sweden)

    Cheryl C Tan

    Full Text Available Polycomb (PcG and trithorax (trxG proteins play important roles in establishing lineage-specific genetic programs through induction of chromatin modifications that lead to gene silencing or activation. Previously, we described an association between the MLL/SET1 complexes and a highly restricted, gene-specific DNA-binding protein Ap2delta that is required for recruitment of the MLL/SET1 complex to target Hoxc8 specifically. Here, we reduced levels of Ap2delta and Ash2l in the neuroblastoma cell line, Neuro2A, and analyzed their gene expression profiles using whole-genome mouse cDNA microarrays. This analysis yielded 42 genes that are potentially co-regulated by Ap2delta and Ash2l, and we have identified evolutionarily conserved Ap2-binding sites in 20 of them. To determine whether some of these were direct targets of the Ap2delta-Ash2l complex, we analyzed several promoters for the presence of Ap2delta and Ash2l by chromatin immunoprecipitation (ChIP. Among the targets we screened, we identified Fgfr3 as a direct transcriptional target of the Ap2delta-Ash2l complex. Additionally, we found that Ap2delta is necessary for the recruitment of Ash2l-containing complexes to this promoter and that this recruitment leads to trimethylation of lysine 4 of histone H3 (H3K4me3. Thus, we have identified several candidate targets of complexes containing Ap2delta and Ash2l that can be used to further elucidate their roles during development and showed that Fgfr3 is a novel direct target of these complexes.

  2. Hot Topics in Study and Application of Steel Line Pipe for Natural Gas Transportation%天然气输送钢管研究与应用中的几个热点问题

    Institute of Scientific and Technical Information of China (English)

    李鹤林

    2001-01-01

    Several hot topics in study and application of steel line pipefor natural gas transportation are introduced including transportation under high pressure ,research and development of high grade steel line pipe,line pipe with high HIC(Hydrogen Induced Cracking) resistant and its technical requirements,rich gas transportation and technical requirements of steel pipe,comparison and selection between longitudinally submerged arc welded pipe and spirally submerged arc welded pipe,ductile fracture arrest of steel pipe for gas transportation,SCC (Stress Corrosion Cracking) of pipeline in soil environment with approximately neutral pH value,residual stress and its control of line pipe,the ratio of yield to tensile strength of high strength steel line pipe,fitness-for-purpose assessment and risk management of pipeline in service,etc..%全面阐述了天然气输送钢管研究与应用中的热点问题:高压输送与高钢级管线钢的研究开发;抗HIC管线管及其技术条件;富气输送及其对钢管性能的要求;直缝埋弧焊管和螺旋缝埋弧焊管的比较与选择;输气钢管延性断裂的止裂;管线在近中性pH土壤环境中的应力腐蚀开裂;管线管的残余应力及控制;高强度管线钢的屈强比问题;在役管道的适用性评价及风险管理等。

  3. AP600 containment purge radiological analysis

    Energy Technology Data Exchange (ETDEWEB)

    O`Connor, M.; Schulz, J.; Tan, C. [Bechtel Power Corporation (United States)] [and others

    1995-02-01

    The AP600 Project is a passive pressurized water reactor power plant which is part of the Design Certification and First-of-a-Kind Engineering effort under the Advanced Light Water Reactor program. Included in this process is the design of the containment air filtration system which will be the subject of this paper. We will compare the practice used by previous plants with the AP600 approach to meet the goals of industry standards in sizing the containment air filtration system. The radiological aspects of design are of primary significance and will be the focus of this paper. The AP600 Project optimized the design to combine the functions of the high volumetric flow rate, low volumetric flow rate, and containment cleanup and other filtration systems into one multi-functional system. This achieves a more simplified, standardized, and lower cost design. Studies were performed to determine the possible concentrations of radioactive material in the containment atmosphere and the effectiveness of the purge system to keep concentrations within 10CFR20 limits and within offsite dose objectives. The concentrations were determined for various reactor coolant system leakage rates and containment purge modes of operation. The resultant concentrations were used to determine the containment accessibility during various stages of normal plant operation including refueling. The results of the parametric studies indicate that a dual train purge system with a capacity of 4,000 cfm per train is more than adequate to control the airborne radioactivity levels inside containment during normal plant operation and refueling, and satisfies the goals of ANSI/ANS-56.6-1986 and limits the amount of radioactive material released to the environment per ANSI/ANS 59.2-1985 to provide a safe environment for plant personnel and offsite residents.

  4. Proactive AP Selection Method Considering the Radio Interference Environment

    Science.gov (United States)

    Taenaka, Yuzo; Kashihara, Shigeru; Tsukamoto, Kazuya; Yamaguchi, Suguru; Oie, Yuji

    In the near future, wireless local area networks (WLANs) will overlap to provide continuous coverage over a wide area. In such ubiquitous WLANs, a mobile node (MN) moving freely between multiple access points (APs) requires not only permanent access to the Internet but also continuous communication quality during handover. In order to satisfy these requirements, an MN needs to (1) select an AP with better performance and (2) execute a handover seamlessly. To satisfy requirement (2), we proposed a seamless handover method in a previous study. Moreover, in order to achieve (1), the Received Signal Strength Indicator (RSSI) is usually employed to measure wireless link quality in a WLAN system. However, in a real environment, especially if APs are densely situated, it is difficult to always select an AP with better performance based on only the RSSI. This is because the RSSI alone cannot detect the degradation of communication quality due to radio interference. Moreover, it is important that AP selection is completed only on an MN, because we can assume that, in ubiquitous WLANs, various organizations or operators will manage APs. Hence, we cannot modify the APs for AP selection. To overcome these difficulties, in the present paper, we propose and implement a proactive AP selection method considering wireless link condition based on the number of frame retransmissions in addition to the RSSI. In the evaluation, we show that the proposed AP selection method can appropriately select an AP with good wireless link quality, i.e., high RSSI and low radio interference.

  5. Ozone mitigation tests at the APS

    Energy Technology Data Exchange (ETDEWEB)

    Kuzay, T.M.; Collins, J.T.; Pisharody, M.; Job, P.K.; Wang Zhibi

    1996-09-01

    Ozone is generated in the APS experimental stations whenever the x-ray beam has a chance to interact with air. Ozone concentrations in an experimental station have to be below a certain defined limit (current OSHA regulations specify 0.08 ppm as the maximum limit) before an experimenter can reenter the hutch. This limit is said to be currently under study for a downward adjustment. One method of depleting the ozone generated in an experimental station is mitigation through either adsorption or direct destruction. In recent tests, both methods were tried using commercially available units. Test results and some analytical predictions are presented.

  6. Wiley AP english language and composition

    CERN Document Server

    Woods, Geraldine

    2013-01-01

    Two full-length practice tests to help you score your highest Review questions with complete answers and explanations for the most popular themes on the test Study strategies and expert test-taking tips to keep you calm and focused on test day Review key exam topics and themes to maximize your score Preparing for the AP English Language and Composition test? This test-prep guide helps you perfect the skills you need to achieve your best possible score. You'll find winning test-taking tips, multiple-choice strategies, and essay guidelines, as well as great advice on getting the

  7. Path Deviation Equations in AP-Geometry

    CERN Document Server

    Wanas, M I

    2005-01-01

    Recently, it has been shown that Absolute Parallelism (AP) geometry admits paths that are naturally quantized. These paths have been used to describe the motion of spinning particles in a background gravitational field. In case of a weak static gravitational field limits, the paths are applied successfully to interpret the discrepancy in the motion of thermal neutrons in the Earth's gravitational field (COW-experiment). The aim of the present work is to explore the properties of the deviation equations corresponding to these paths. In the present work the deviation equations are derived and compared to the geodesic deviation equation of the Riemannian geometry.

  8. Evaluation of environmental impact produced by power distribution lines. Evaluacion de impacto ambiental de lineas aereas de transporte de energia electrica

    Energy Technology Data Exchange (ETDEWEB)

    Granadino Asenjo, A.; Fernandez Teran, E. (INTRADEL-Grupo Cobra (Spain))

    1993-01-01

    In Spain the analysis and evaluation of the environmental impact produced by power distribution lines (aerial) is carried out under Regional Governments regulations. In general, a description of the line characteristics and those actions and works that during assembly and installation might produce environmental alterations are evaluated. The method used to quantify all these effects is given. (Author)

  9. Spent fuel pool spray cooling system for the AP1000 {sup registered}

    Energy Technology Data Exchange (ETDEWEB)

    Vujic, Zoran; Sassen, Felix; Tietsch, Wolfgang [Westinghouse Electric Germany GmbH, Mannheim (Germany)

    2013-07-01

    The AP1000 {sup registered} plant design features multiple, diverse lines of defense to ensure spent fuel cooling can be maintained for Design Basis Events and Beyond Design Basis Accidents (BDBA). The AP1000 {sup registered} plant lines of defense with respect to Spent Fuel Pool (SFP) cooling are as follows: 1. During normal and abnormal conditions, defense-in-depth and duty systems provide highly reliable SFP cooling, supplied by offsite AC power or the onsite Standby Diesel Generators. 2. For unlikely events with extended loss of AC power (i.e. station black-out) and/or loss of heat sink, spent fuel cooling can be still provided indefinitely by: 2a. Passive systems, requiring minimal or no operator actions, sufficient for at least 72 hours under all possible loading conditions. 2b. After 3 days, several different means are provided to continue SFP cooling using installed plant equipment as well as off-site equipment with built-in connections. 3. Even for BDBA with postulated SFP damage and multiple failures in the passive safety-related systems and in the defense-in-depth active systems, the AP1000 {sup registered} SFP Spray System provides an additional line of defense to prevent spent fuel damage. (orig.)

  10. APS insertion devices: Recent developments and results

    International Nuclear Information System (INIS)

    The Advanced Photon Source (APS) now has a total of 23 insertion devices (IDs). Over two-thirds of them are installed on the storage ring. The installed devices include 18, 27 and 55 mm-period undulators; an 85 mm-period wiggler; a 16 cm-period elliptical multipole wiggler; and many 33 mm-period undulators. Most of the IDs occupy storage-ring straight sections equipped with 8 mm vertical-aperture vacuum chambers. All of the IDs were measured magnetically at the APS and, in most cases, underwent a final magnetic tuning in order to minimize variation in the various integrals of the field through the ID over the full gap range. Special shimming techniques to correct magnetic field parameters in appropriate gap-dependent ways were developed and applied. Measurements of the closed-orbit distortion as a function of the ID gap variation have been completed, and results are in a good agreement with magnetic measurements. Spectral diagnostics of the ID radiation, including measurements of the absolute spectral flux, brilliance and polarization, show excellent agreement between calculated and measured results. Studies of the sensitivity of IDs to radiation exposure and measurements of the dose rate received by the IDs are in progress

  11. Dicty_cDB: FC-AP24 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP24 (Link to dictyBase) - - - Contig-U16528-1 FC-AP24F (Li...nk to Original site) FC-AP24F 525 - - - - - - Show FC-AP24 Library FC (Link to library) Clone ID FC-AP24 (Li.../dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP24Q.Seq.d/ Representative seq. ID FC-AP...24F (Link to Original site) Representative DNA sequence >FC-AP24 (FC-AP24Q) /CSM/FC/FC-AP/FC-AP24Q.Seq....C-BE24Q.Seq.d/ 1041 0.0 FC-AP24 (FC-AP24Q) /CSM/FC/FC-AP/FC-AP24Q.Seq.d/ 1041 0.0

  12. Dicty_cDB: FC-AP21 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP21 (Link to dictyBase) - - - Contig-U15099-1 FC-AP21Z (Li...nk to Original site) - - FC-AP21Z 511 - - - - Show FC-AP21 Library FC (Link to library) Clone ID FC-AP21 (Li.../dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP21Q.Seq.d/ Representative seq. ID FC-AP...21Z (Link to Original site) Representative DNA sequence >FC-AP21 (FC-AP21Q) /CSM/FC/FC-AP/FC-AP21Q.Seq....14Q.Seq.d/ 1013 0.0 SLE553 (SLE553Q) /CSM/SL/SLE5-C/SLE553Q.Seq.d/ 1013 0.0 FC-AP21 (FC-AP21Q) /CSM/FC/FC-AP/FC-AP

  13. Dicty_cDB: FC-AP22 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP22 (Link to dictyBase) - G24045 DDB0232387 Contig-U15141-1 FC-AP...22F (Link to Original site) FC-AP22F 317 - - - - - - Show FC-AP22 Library FC (Link to library) Clone ID FC-AP...1-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP22Q.Se...q.d/ Representative seq. ID FC-AP22F (Link to Original site) Representative DNA sequence >FC-AP22 (FC-AP22Q) /CSM/FC/FC-AP/FC-AP...KKKKKKK Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value FC-AP22 (FC-AP22Q) /CSM/FC/FC-AP/FC-AP

  14. Determination of absolute transport efficiencies of Be, Cd, In, Pb and Bi for electrothermal vaporization sample introduction into an inductively coupled plasma using an in-line electrostatic precipitator

    Energy Technology Data Exchange (ETDEWEB)

    Ertas, Gulay; Holcombe, James A. E-mail: holcombe@mail.utexas.edu

    2003-09-26

    A novel laboratory-constructed electrostatic precipitator for the determination of analyte transport efficiencies from a tube furnace electrothermal vaporization (ETV) sample introduction system is described. This simple experimental set up captures the analyte electrostatically using a negative corona discharge. The absolute transport efficiencies using experimental conditions typical of those applied to real analysis by ETV-ICP-MS were determined for Be, Cd, In, Pb and Bi to be 26{+-}3, 39{+-}8, 35{+-}1, 50{+-}11 and 35{+-}1%, respectively. Pd and NaCl were evaluated as 'carriers' and were found to only marginally increase analyte transport for the elements investigated. Pd enhanced efficiencies 20-50% for all but Bi, which decreased by 17%. The NaCl produced transport enhancements of 10-50% for all five analytes. However, uncertainties in the measurements made several of the individual improvements statistically insignificant. The mean precipitator collection efficiency of the aerosol leaving the ETV for the five elements studied was found to be greater than 90% and was determined using the ICP-MS signal with the in-line precipitator's voltage either on or off. While 30 ETV firings were used for a single efficiency determination, reasonable precision could be obtained with approximately 10 firings while still employing reasonable analyte masses, i.e. approximately 20 ng. The transport results are in good agreement with previous studies using similar ETV design and analyte masses.

  15. Butyrate produced by commensal bacteria potentiates phorbol esters induced AP-1 response in human intestinal epithelial cells.

    Directory of Open Access Journals (Sweden)

    Malgorzata Nepelska

    Full Text Available The human intestine is a balanced ecosystem well suited for bacterial survival, colonization and growth, which has evolved to be beneficial both for the host and the commensal bacteria. Here, we investigated the effect of bacterial metabolites produced by commensal bacteria on AP-1 signaling pathway, which has a plethora of effects on host physiology. Using intestinal epithelial cell lines, HT-29 and Caco-2, stably transfected with AP-1-dependent luciferase reporter gene, we tested the effect of culture supernatant from 49 commensal strains. We observed that several bacteria were able to activate the AP-1 pathway and this was correlated to the amount of short chain fatty acids (SCFAs produced. Besides being a major source of energy for epithelial cells, SCFAs have been shown to regulate several signaling pathways in these cells. We show that propionate and butyrate are potent activators of the AP-1 pathway, butyrate being the more efficient of the two. We also observed a strong synergistic activation of AP-1 pathway when using butyrate with PMA, a PKC activator. Moreover, butyrate enhanced the PMA-induced expression of c-fos and ERK1/2 phosphorylation, but not p38 and JNK. In conclusion, we showed that SCFAs especially butyrate regulate the AP-1 signaling pathway, a feature that may contribute to the physiological impact of the gut microbiota on the host. Our results provide support for the involvement of butyrate in modulating the action of PKC in colon cancer cells.

  16. Road and Street Centerlines - INTERSTATES_I_69_INDOT_IN: Interstate Extension for I-69 in Southern Indiana (Indiana Department of Transportation, Line Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — INTERSTATES_I_69_INDOT_IN is a line shapefile that contains the newly developed I-69 route and associated ramps in southern Indiana, from Evansville to Bloomington....

  17. Light Rail and Subway Lines, This layer represents MTA's Lightrail transportation network., Published in 2007, 1:12000 (1in=1000ft) scale, City of Baltimore.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Light Rail and Subway Lines dataset, published at 1:12000 (1in=1000ft) scale, was produced all or in part from Field Observation information as of 2007. It is...

  18. Phosphatidylcholine passes through lateral tight junctions for paracellular transport to the apical side of the polarized intestinal tumor cell-line CaCo2.

    Science.gov (United States)

    Stremmel, Wolfgang; Staffer, Simone; Gan-Schreier, Hongying; Wannhoff, Andreas; Bach, Margund; Gauss, Annika

    2016-09-01

    Phosphatidylcholine (PC) is the most abundant phospholipid in intestinal mucus, indicative of a specific transport system across the mucosal epithelium to the intestinal lumen. To elucidate this transport mechanism, we employed a transwell tissue culture system with polarized CaCo2 cells. It was shown that PC could not substantially be internalized by the cells. However, after basal application of increasing PC concentrations, an apical transport of 47.1±6.3nmolh(-1)mMPC(-1) was observed. Equilibrium distribution studies with PC applied in equal concentrations to the basal and apical compartments showed a 1.5-fold accumulation on the expense of basal PC. Disruption of tight junctions (TJ) by acetaldehyde or PPARγ inhibitors or by treatment with siRNA to TJ proteins suppressed paracellular transport by at least 50%. Transport was specific for the choline containing the phospholipids PC, lysoPC and sphingomyelin. We showed that translocation is driven by an electrochemical gradient generated by apical accumulation of Cl(-) and HCO3(-) through CFTR. Pretreatment with siRNA to mucin 3 which anchors in the apical plasma membrane of mucosal cells inhibited the final step of luminal PC secretion. PC accumulates in intestinal mucus using a paracellular, apically directed transport route across TJs. PMID:27365309

  19. Dicty_cDB: FC-AP13 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP13 (Link to dictyBase) - - - Contig-U15374-1 FC-AP13P (Li...nk to Original site) FC-AP13F 550 FC-AP13Z 184 FC-AP13P 734 - - Show FC-AP13 Library FC (Link to library) Clone ID FC-AP...al site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP13Q.Seq.d/ Representative seq. ID FC-AP...13P (Link to Original site) Representative DNA sequence >FC-AP13 (FC-AP13Q) /CSM/FC/FC-AP/FC-AP...KKRKLNILIIII*fnnhqvmekkikkkiknkkn f*k Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value FC-AP

  20. Transmission Lines or Poles, Electric, MDTA High Mast lighting, High Mast Lighting along I 95, Maryland Transportation Authority High Mast Lighting poles, Published in 2011, 1:1200 (1in=100ft) scale, Maryland Transportation Authority.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Transmission Lines or Poles, Electric dataset, published at 1:1200 (1in=100ft) scale, was produced all or in part from Orthoimagery information as of 2011. It...

  1. Adaptor protein complexes AP-1 and AP-3 are required by the HHV-7 Immunoevasin U21 for rerouting of class I MHC molecules to the lysosomal compartment.

    Directory of Open Access Journals (Sweden)

    Lisa A Kimpler

    Full Text Available The human herpesvirus-7 (HHV-7 U21 gene product binds to class I major histocompatibility complex (MHC molecules and reroutes them to a lysosomal compartment. Trafficking of integral membrane proteins to lysosomes is mediated through cytoplasmic sorting signals that recruit heterotetrameric clathrin adaptor protein (AP complexes, which in turn mediate protein sorting in post-Golgi vesicular transport. Since U21 can mediate rerouting of class I molecules to lysosomes even when lacking its cytoplasmic tail, we hypothesize the existence of a cellular protein that contains the lysosomal sorting information required to escort class I molecules to the lysosomal compartment. If such a protein exists, we expect that it might recruit clathrin adaptor protein complexes as a means of lysosomal sorting. Here we describe experiments demonstrating that the μ adaptins from AP-1 and AP-3 are involved in U21-mediated trafficking of class I molecules to lysosomes. These experiments support the idea that a cellular protein(s is necessary for U21-mediated lysosomal sorting of class I molecules. We also examine the impact of transient versus chronic knockdown of these adaptor protein complexes, and show that the few remaining μ subunits in the cells are eventually able to reroute class I molecules to lysosomes.

  2. Response to zinc deficiency of two rice lines with contrasting tolerance is determined by root growth maintenance and organic acid exudation rates, and not by zinc-transporter activity.

    Science.gov (United States)

    Widodo, Basuki; Broadley, Martin R; Rose, Terry; Frei, Michael; Pariasca-Tanaka, Juan; Yoshihashi, Tadashi; Thomson, Michael; Hammond, John P; Aprile, Alessio; Close, Timothy J; Ismail, Abdelbagi M; Wissuwa, Matthias

    2010-04-01

    *Zinc (Zn)-deficient soils constrain rice (Oryza sativa) production and cause Zn malnutrition. The identification of Zn-deficiency-tolerant rice lines indicates that breeding might overcome these constraints. Here, we seek to identify processes underlying Zn-deficiency tolerance in rice at the physiological and transcriptional levels. *A Zn-deficiency-tolerant line RIL46 acquires Zn more efficiently and produces more biomass than its nontolerant maternal line (IR74) at low [Zn](ext) under field conditions. We tested if this was the result of increased expression of Zn(2+) transporters; increased root exudation of deoxymugineic acid (DMA) or low-molecular-weight organic acids (LMWOAs); and/or increased root production. Experiments were performed in field and controlled environment conditions. *There was little genotypic variation in transcript abundance of Zn-responsive root Zn(2+)-transporters between the RIL46 and IR74. However, root exudation of DMA and LMWOA was greater in RIL46, coinciding with increased root expression of putative ligand-efflux genes. Adventitious root production was maintained in RIL46 at low [Zn](ext), correlating with altered expression of root-specific auxin-responsive genes. *Zinc-deficiency tolerance in RIL46 is most likely the result of maintenance of root growth, increased efflux of Zn ligands, and increased uptake of Zn-ligand complexes at low [Zn](ext); these traits are potential breeding targets. PMID:20100202

  3. Market analysis of APS/SCM applications and technologies

    OpenAIRE

    Prášil, Zdeněk

    2009-01-01

    The bachelor thesis aims to describe a current worldwide market of APS/SCM technologies and applications , i.e. advanced planned scheduling /supply chain management. In the first part, the APS/SCM is described in theory. The APS/SCM is defined and its benefits and impacts on company are discussed. The next part of the work is focused on the market with APS/SCM and the distribution of forces in the market. The demand and supply of this market segment is analyzed. In the last part, solutions of...

  4. Project W-211, initial tank retrieval systems, description of operations for 241-AP-102 and 241-AP-104

    Energy Technology Data Exchange (ETDEWEB)

    RIECK, C.A.

    1999-02-25

    The primary purpose of the Initial Tank Retrieval Systems (ITRS) is to provide systems for retrieval of radioactive wastes stored in underground double-shell tanks (DSTS) for transfer to alternate storage, evaporation, pretreatment or treatment, while concurrently reducing risks associated with safety watch list and other DSTs. This Description of Operations (DOO) defines the control philosophy for the waste retrieval system for tanks 241-AP-102 (AP-102) and 241-AP-104 (AP-104). This DOO will provide a basis for the detailed design of the Retrieval Control System (RCS) for AP-102 and AP-104 and establishes test criteria for the RCS. The test criteria will be used during qualification testing and acceptance testing to verify operability.

  5. AP1000主系统先进性分析%The Progressiveness Analysis of the AP1000 Main System

    Institute of Scientific and Technical Information of China (English)

    李家杰

    2012-01-01

      本文总结了第三代先进非能动压水堆核电厂AP1000主系统的主要特点,对AP1000主系统的先进性进行了分析,核电厂主系统设计人员能够从该文中了解到 AP1000主系统与第二代核电厂主系统之间的差别。%  This paper summarizes the main features of the AP1000 main system of the third generation of advanced passive pressurized water reactor nuclear power plant , and analyzes the progressiveness of the AP1000 main system .From this paper, the main system designers of nuclear power factory can learn the difference between the second-generation nuclear power factory main system and AP1000 main system

  6. Characterization of liquid entrainment in the AP1000 automatic depressurization system from APEX tests

    International Nuclear Information System (INIS)

    Full text of publication follows: The AP1000 is a 1000 MWe advanced nuclear power plant that uses passive safety features to enhance plant safety and to provide significant and measurable improvements in plant simplification, reliability, investment protection and plant costs. The AP1000 relies heavily on the 600 MWe AP600 which received design certification in 1999. A critical part of the AP600 design certification process involved the testing of the passive safety systems. A one-fourth height, one-fourth pressure test facility, APEX-600, was constructed at the Oregon State University to study design basis events, and to provide a body of data to be used to validate the computer models used to analyze the AP600. This facility was extensively modified to reflect the design changes for AP1000 including higher power in the electrically heated rods representing the reactor core, and changes in the size of the pressurizer, core makeup tanks and automatic depressurization system. The APEX-1000 test facility was used to perform design basis accident simulations and separate effects tests to support the AP1000 design certification process. In the event of a LOCA, the AP1000 passive core cooling system provides sources of core makeup water along with an automatic depressurization system (ADS) consisting of several stages of valves which reduce the reactor coolant system pressure in a controlled manner. The final stage of this system, ADS-4, consists of four large valves that open off the hot legs, reducing the pressure to allow gravity injection from the in-containment refueling water storage tank (IRWST) and eventually the containment sump. The 67% increase in power from AP600 to AP1000 results in proportionally larger steam velocities exiting the core. Higher steam velocities could increases the potential for significant liquid entrainment out the ADS-4 lines, affecting the liquid inventory in the reactor. Tests were performed in APEX-1000 to characterize the two

  7. Characterization of liquid entrainment in the AP1000 automatic depressurization system from APEX tests

    Energy Technology Data Exchange (ETDEWEB)

    Richard F Wright; Terry L Schulz [Westinghouse Electric Co., PO Box 355, Pittsburgh, PA (United States); Jose N Reyes; John Groome [Oregon State University, Corvallis, OR (United States)

    2005-07-01

    Full text of publication follows: The AP1000 is a 1000 MWe advanced nuclear power plant that uses passive safety features to enhance plant safety and to provide significant and measurable improvements in plant simplification, reliability, investment protection and plant costs. The AP1000 relies heavily on the 600 MWe AP600 which received design certification in 1999. A critical part of the AP600 design certification process involved the testing of the passive safety systems. A one-fourth height, one-fourth pressure test facility, APEX-600, was constructed at the Oregon State University to study design basis events, and to provide a body of data to be used to validate the computer models used to analyze the AP600. This facility was extensively modified to reflect the design changes for AP1000 including higher power in the electrically heated rods representing the reactor core, and changes in the size of the pressurizer, core makeup tanks and automatic depressurization system. The APEX-1000 test facility was used to perform design basis accident simulations and separate effects tests to support the AP1000 design certification process. In the event of a LOCA, the AP1000 passive core cooling system provides sources of core makeup water along with an automatic depressurization system (ADS) consisting of several stages of valves which reduce the reactor coolant system pressure in a controlled manner. The final stage of this system, ADS-4, consists of four large valves that open off the hot legs, reducing the pressure to allow gravity injection from the in-containment refueling water storage tank (IRWST) and eventually the containment sump. The 67% increase in power from AP600 to AP1000 results in proportionally larger steam velocities exiting the core. Higher steam velocities could increases the potential for significant liquid entrainment out the ADS-4 lines, affecting the liquid inventory in the reactor. Tests were performed in APEX-1000 to characterize the two

  8. Decision Support Facility for the APS Control System

    CERN Document Server

    Dohan, D A

    2001-01-01

    The Advanced Photon Source is now in its fifth year of routine beam production. The EPICS-based [1] control system has entered the phase in its life cycle where new control algorithms must be implemented under increasingly stringent operational and reliability requirements. The sheer volume of the control system (~270,000 records, ~145 VME-based input-output controllers (IOCs), and ~7,000,000 lines of EPICS ASCII configuration code), presents a daunting challenge for code maintenance. The present work describes a relational database that provides an integrated view of the interacting components of the entire APS control system, including the IOC low-level logic, the physical wiring documentation, and high-level client applications. The database is extracted (booted) from the same operational CVS repository as that used to load the active IOCs. It provides site-wide decision support facilities to inspect and trace control flow and to identify client (e.g., user interface) programs involved at any selected poin...

  9. Decision support facility for the APS control system.

    Energy Technology Data Exchange (ETDEWEB)

    Dohan, D. A.

    2001-11-12

    The Advanced Photon Source is now in its fifth year of routine beam production. The EPICS-based [1] control system has entered the phase in its life cycle where new control algorithms must be implemented under increasingly stringent operational and reliability requirements. The sheer volume of the control system ({approx}270,000 records, {approx}145 VME-based input-output controllers (IOCs), and {approx}7,000,000 lines of EPICS ASCII configuration code), presents a daunting challenge for code maintenance. The present work describes a relational database that provides an integrated view of the interacting components of the entire APS control system, including the IOC low-level logic, the physical wiring documentation, and high-level client applications. The database is extracted (booted) from the same operational CVS repository as that used to load the active IOCs. It provides site-wide decision support facilities to inspect and trace control flow and to identify client (e.g., user interface) programs involved at any selected point in the front-end logic. The relational database forms a basis for generalized documentation of global control logic and its connection with both the physical I/O and with external high-level applications.

  10. Genome-Wide Identification of the Target Genes of AP2-O, a Plasmodium AP2-Family Transcription Factor.

    Directory of Open Access Journals (Sweden)

    Izumi Kaneko

    2015-05-01

    Full Text Available Stage-specific transcription is a fundamental biological process in the life cycle of the Plasmodium parasite. Proteins containing the AP2 DNA-binding domain are responsible for stage-specific transcriptional regulation and belong to the only known family of transcription factors in Plasmodium parasites. Comprehensive identification of their target genes will advance our understanding of the molecular basis of stage-specific transcriptional regulation and stage-specific parasite development. AP2-O is an AP2 family transcription factor that is expressed in the mosquito midgut-invading stage, called the ookinete, and is essential for normal morphogenesis of this stage. In this study, we identified the genome-wide target genes of AP2-O by chromatin immunoprecipitation-sequencing and elucidate how this AP2 family transcription factor contributes to the formation of this motile stage. The analysis revealed that AP2-O binds specifically to the upstream genomic regions of more than 500 genes, suggesting that approximately 10% of the parasite genome is directly regulated by AP2-O. These genes are involved in distinct biological processes such as morphogenesis, locomotion, midgut penetration, protection against mosquito immunity and preparation for subsequent oocyst development. This direct and global regulation by AP2-O provides a model for gene regulation in Plasmodium parasites and may explain how these parasites manage to control their complex life cycle using a small number of sequence-specific AP2 transcription factors.

  11. APS extends open access to all its journals

    CERN Multimedia

    Thomas, Kim

    2006-01-01

    "Physics research promoter and publisher the American Physical Society (APS) is to extend open access to all its journals. Th APS previously made its five print journals available through subscriptions, and its two e-journals (Physical Review Special Topics and Physics Educatoin Research) on an open access basis." (1/2 page)

  12. AP-42 ADDITIONS AND REVISIONS - LANDFILLS (COMBUSTION CONTROLS)

    Science.gov (United States)

    This project develops emission factors, etc., for landfills, in particular for combustion devices fed by landfill gas, for incorporation into AP-42. AP-42 is a massive collection of information concerning processes which generate air emissions and presents emission factors and co...

  13. Data-Based Decision Making: The Road to AP Equity

    Science.gov (United States)

    Edwards, Kelcey; Duggan, Odette

    2012-01-01

    Presented at the Advanced Placement Annual Conference (APAC) in Lake Buena Vista, FL in July 2012. This presentation reviews concepts central to achieving equitable AP access and success for all willing and academically prepared students. We analyze trends in participation and performance by race/ethnicity from the AP Report to the Nation and…

  14. A Closer Examination of the Academic Benefits of AP

    Science.gov (United States)

    McKillip, Mary E. M.; Rawls, Anita

    2013-01-01

    The authors sought to better understand the relationship between students participating in the Advanced Placement (AP) program and subsequent performance on the Scholastic Aptitude Test (SAT). Focusing on students graduating from U.S. public high schools in 2010, the authors used propensity scores to match junior year AP examinees in 3 subjects to…

  15. Evaluation and Modification of NOTRUMP-AP600 Code With Neglecting Momentum Flux Terms%对 NOTRUMP-AP600程序缺少动量通量项的评估与修正

    Institute of Scientific and Technical Information of China (English)

    樊普; 郑尧瑶

    2014-01-01

    The momentum flux terms are not included in the momentum equations for NOTRUMP-AP600 code .The predicted injection mass flow rate of IRWST and pres-surizer level deviate test results largely when NOTRUMP-AP600 code is used to analyze SBLOCA for APEX-600 test facility . The impact of neglecting the momentum flux terms in the NOTRUMP-AP600 was evaluated ,including the following contents :The impact of neglecting momentum flux terms for the pressure drop of ADS line was calcu-lated using homogenous model and separated flow model ;the detailed two-phase pres-sure drop was calculated using FLOAD4 code for the ADS4 line ,and the result was used to benchmark the ADS4 pressure drop for NOTRUMP-AP600 .The results show that the input resistance coefficient should be increased by 60% for AP600 ADS4 line .%由于NOTRUMP-AP600程序的动量守恒方程缺少动量通量项,在分析用于模拟AP600核电厂的A PEX试验台架小破口事故时,安全壳内置换料水箱注射流量和稳压器混合水位等参数的预测值和实验值有较大偏离。本文对此进行评估:1)采用均相流和分相流模型计算动量通量项对A P600核电厂自动卸压系统(ADS)管路压降的影响;2)采用FLOAD4程序对需修正的第4级ADS(ADS4)管路的两相流压降进行计算,预测ADS4管路内的压力分布,并用作修正NOTRUMP-AP600程序 ADS4管路压降的基准。结果表明,对于A P600核电厂ADS4管路,输入阻力系数需增加60%。

  16. IL12A, MPHOSPH9/CDK2AP1 and RGS1 are novel multiple sclerosis susceptibility loci

    DEFF Research Database (Denmark)

    Sørensen, Per Soelberg

    2010-01-01

    same direction of effect observed in the discovery phase. Three loci exceeded genome-wide significance in the joint analysis: RGS1 (P value=3.55 x 10(-9)), IL12A (P=3.08 x 10(-8)) and MPHOSPH9/CDK2AP1 (P=3.96 x 10(-8)). The RGS1 risk allele is shared with celiac disease (CD), and the IL12A risk allele...... seems to be protective for celiac disease. Within the MPHOSPH9/CDK2AP1 locus, the risk allele correlates with diminished RNA expression of the cell cycle regulator CDK2AP1; this effect is seen in both lymphoblastic cell lines (P=1.18 x 10(-5)) and in peripheral blood mononuclear cells from subjects with...

  17. IL12A, MPHOSPH9/CDK2AP1 and RGS1 are novel multiple sclerosis susceptibility loci

    DEFF Research Database (Denmark)

    Sørensen, Per Soelberg

    2010-01-01

    and the same direction of effect observed in the discovery phase. Three loci exceeded genome-wide significance in the joint analysis: RGS1 (P value=3.55 x 10(-9)), IL12A (P=3.08 x 10(-8)) and MPHOSPH9/CDK2AP1 (P=3.96 x 10(-8)). The RGS1 risk allele is shared with celiac disease (CD), and the IL12A risk allele...... seems to be protective for celiac disease. Within the MPHOSPH9/CDK2AP1 locus, the risk allele correlates with diminished RNA expression of the cell cycle regulator CDK2AP1; this effect is seen in both lymphoblastic cell lines (P=1.18 x 10(-5)) and in peripheral blood mononuclear cells from subjects...... with MS (P=0.01). Thus, we report three new MS susceptibility loci, including a novel inflammatory disease locus that could affect autoreactive cell proliferation....

  18. Enhancing and Archiving the APS Catalog of the POSS I

    Science.gov (United States)

    Humphreys, Roberta M.

    2003-01-01

    We have worked on two different projects: 1) Archiving the APS Catalog of the POSS I for distribution to NASA's NED at IPAC, SIMBAD in France, and individual astronomers and 2) The automated morphological classification of galaxies. We have completed archiving the Catalog into easily readable binary files. The database together with the software to read it has been distributed on DVD's to the national and international data centers and to individual astronomers. The archived Catalog contains more than 89 million objects in 632 fields in the first epoch Palomar Observatory Sky Survey. Additional image parameters not available in the original on-line version are also included in the archived version. The archived Catalog is also available and can be queried at the APS web site (URL: http://aps.umn.edu) which has been improved with a much faster and more efficient querying system. The Catalog can be downloaded as binary datafiles with the source code for reading it. It is also being integrated into the SkyQuery system which includes the Sloan Digital Sky Survey, 2MASS, and the FIRST radio sky survey. We experimented with different classification algorithms to automate the morphological classification of galaxies. This is an especially difficult problem because there are not only a large number of attributes or parameters and measurement uncertainties, but also the added complication of human disagreement about the adopted types. To solve this problem we used 837 galaxy images from nine POSS I fields at the North Galactic Pole classified by two independent astronomers for which they agree on the morphological types. The initial goal was to separate the galaxies into the three broad classes relevant to issues of large scale structure and galaxy formation and evolution: early (ellipticals and lenticulars), spirals, and late (irregulars) with an accuracy or success rate that rivals the best astronomer classifiers. We also needed to identify a set of parameters derived

  19. Native Mobile Ap or Mobile Web Page?

    OpenAIRE

    Yang, Yuxi; Theller, Larry

    2013-01-01

    This project is designed to greatly enhance the lines of communication between the pollinator industry and the pesticide applicator community. It builds on the DriftWatch.org Pesticide Sensitive Crop Registry (www.driftwatch.org), which currently covers nine states, with a customized map for each state (Colorado, Indiana, Illinois, Michigan, Missouri, Minnesota, Montana, Nebraska, and Wisconsin).

  20. Dicty_cDB: FC-AP05 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP05 (Link to dictyBase) - - - Contig-U16521-1 FC-AP05Z (Li...nk to Original site) - - FC-AP05Z 532 - - - - Show FC-AP05 Library FC (Link to library) Clone ID FC-AP05 (Li.../dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP05Q.Seq.d/ Representative seq. ID FC-AP...05Z (Link to Original site) Representative DNA sequence >FC-AP05 (FC-AP05Q) /CSM/FC/FC-AP/FC-AP05Q.Seq....GAGA TCCGTCAAAGTTTCAAGCAAAAAAGTTGTTGCCAAGTAAATAAATAATACTTTTTTCCCT AT sequence update 1997. 3.25 Translated Amino Acid sequence ---AP

  1. Dicty_cDB: FC-AP23 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP23 (Link to dictyBase) - G03230 DDB0190153 Contig-U16094-1 FC-AP...23P (Link to Original site) FC-AP23F 515 FC-AP23Z 472 FC-AP23P 987 - - Show FC-AP23 Library FC (Link ...ontig Contig-U16094-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP...23Q.Seq.d/ Representative seq. ID FC-AP23P (Link to Original site) Representative DNA sequence >FC-AP23 (FC-AP...23Q) /CSM/FC/FC-AP/FC-AP23Q.Seq.d/ CAAATACATAATCTCTTTTTTGAAAATGTCCGAAAATAACGAAATTGAAATGGAACTCC

  2. Dicty_cDB: FC-AP12 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP12 (Link to dictyBase) - G01739 DDB0232214 Contig-U15924-1 FC-AP...12P (Link to Original site) FC-AP12F 323 FC-AP12Z 613 FC-AP12P 936 - - Show FC-AP12 Library FC (Link ...ontig Contig-U15924-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP...12Q.Seq.d/ Representative seq. ID FC-AP12P (Link to Original site) Representative DNA sequence >FC-AP12 (FC-AP...12Q) /CSM/FC/FC-AP/FC-AP12Q.Seq.d/ CATATTATTTTAAATTTCAGATGTTCCCAAAATAACACATTAATTTGCTTTTTTTGTTG

  3. AP1000R licensing and deployment in the United States

    International Nuclear Information System (INIS)

    In recent years, both domestic and foreign utilities have turned to the standardized Westinghouse AP1000 plant design in satisfying their near - and long-term - sustainable energy needs. As direct support to these actions, licensing the AP1000 design has played a significant role by providing one of the fundamental bases in clearing regulatory hurdles leading to the start of new plant construction. Within the U.S. alone, Westinghouse AP1000 licensing activities have reached unprecedented milestones with the approvals of both AP1000 Design Certification and Southern Company's combined construction permit and operating license (COL) application directly supporting the construction of two new nuclear plants in Georgia. Further COL application approvals are immediately pending for an additional two AP1000 plants in South Carolina. And, across the U.S. nuclear industry spectrum, there are 10 other COL applications under regulatory review representing some 16 new plants at 10 sites. In total, these actions represent the first wave of new plant licensing under the regulatory approval process since 1978. Fundamental to the Nuclear Regulatory Commission's AP1000 Design Certification is the formal recognition of the AP1000 passive safety design through regulatory acceptance rulemaking. Through recognition and deployment of the AP1000 Design Certification, the utility licensee / operator of this reactor design are now offered an opportunity to use a simplified 'one-step' combined license process, thereby managing substantial back-end construction schedule risk from regulatory and intervention delays. Application of this regulatory philosophy represents both acceptance and encouragement of standardized reactor designs like the AP1000. With the recent AP1000 Design Certification and utility COL acceptances, the fundamental licensing processes of this philosophy have successfully proven the attainment of significant milestones with the next stage licensing actions directed

  4. Estradiol dependent anchoring of the goat uterine estrogen receptor activation factor (E-RAF) at the endoplasmic reticulum by a 55 kDa anchor protein (ap55).

    Science.gov (United States)

    Govind, Anitha P; Sreeja, S; Thampan, Raghava Varman

    2003-05-01

    The primary intracellular site of localization of the estrogen receptor activation factor (E-RAF) is shown here to be the endoplasmic reticulum where the protein remains anchored through an estrogen dependent mechanism. The retention of E-RAF by the endoplasmic reticulum is facilitated by two proteins: (1) a 55 kDa anchor protein (ap55) which is an integral membrane protein of the endoplasmic reticulum. ap55 is a high affinity estrogen binding protein. A conformational change induced by estrogen binding is thought to favor the anchoring process. (2) The anchoring of E-RAF by ap55 is mediated by yet another protein. This is the 66 kDa transport protein (tp66) which recognizes ap55 on the one hand and E-RAF on the other. The presence of estradiol that saturates the hormone binding sites on ap55 appears to favor the anchoring of tp66-E-RAF complex to ap55. This interaction appears to be weakened by levels of estradiol below 7 nM concentration leading to the dissociation of the tp66-E-RAF complex from ap55. The tp66-E-RAF complex moves towards the nucleus. PMID:12682911

  5. Continuous on-line calibration of diffusive soil-atmosphere trace gas transport using vertical {sup 220}Rn- and {sup 222}Rn-activity profiles

    Energy Technology Data Exchange (ETDEWEB)

    Lehmann, B.E. [Bern Univ. (Switzerland). Physics Inst.; Neftel, A. [Inst. of Environmental Protection and Agriculture, Bern (Switzerland); Tarakanov, S.V. [Inst. of Silicate Chemistry, St. Petersburg (Russian Federation)

    2001-07-01

    Continuous monitoring of {sup 220}Rn- and {sup 222}Rn-activities above and below the soil surface combined with sporadic direct {sup 222}Rn-flux measurements is used to quantify diffusive trace gas transport in the air-filled pore space of soil, through the soil-atmosphere interface and in the lowest layers of the atmosphere. In a calm night, {sup 222}Rn-activities above the surface first build-up near the ground (z < 10 cm) and subsequently with a delay of 2-3 hours at higher altitudes (z < 5 m). Knowing (1) the {sup 222}Rn-flux from activity profiles measured in soil gas, (2) from direct flux determinations and (3) using information about atmospheric diffusion parameters from {sup 220}Rn-activities measured near the surface it is possible to model the temporal evolution of the vertical {sup 222}Rn-profiles in a night with stable weather and constant soil conditions. The system operates automatically for extended periods of time in the field enabling a better understanding of transport processes in response to changing environmental conditions (wind, rain, soil humidity). (orig.)

  6. Different involvement of promoter methylation in the expression of organic cation/carnitine transporter 2 (OCTN2 in cancer cell lines.

    Directory of Open Access Journals (Sweden)

    Qiang Qu

    Full Text Available Organic cation/carnitine transporter 2 (OCTN2 is responsible for the cellular uptake of the antineoplastic agent, oxaliplatin. Epigenetic modification is a possible mechanism of altered drug-transporter expression in cancers, leading to altered efficacy of chemotherapeutic drugs. However, the mechanisms governing OCTN2 regulation are not completely understood. In this study, the low levels of OCTN2 in HepG2 and LS174T cells were elevated by the demethylating reagent, decitabine (DCA. To further reveal the epigenetic mechanism of down-regulation of OCTN2, we found that Region-1 within the OCTN2 promoter (spanning -354 to +85 was a determinant of OCTN2 expression in a luciferase reporter assay. Moreover, methylation-specific PCR (MSP and bisulfite genomic sequencing showed that the degree of individual methylated CpG sites within this region was inversely correlated with the levels of OCTN2 in different cancer cells. Application of DCA to HepG2 and LS174T cells reversed the hypermethylation status of the OCTN2 promoter and increased OCTN2 expression, enhancing cellular uptake of oxaliplatin. Thus, we identified that promoter methylation is responsible for epigenetic down-regulation of OCTN2 in HepG2 and LS174T cells. Given the essential role of OCTN2 in cancer cell uptake of chemotherapeutics, and thus treatment efficacy, pretreatment with a demethylating reagent is a possible strategy for optimizing pharmacotherapies against cancers.

  7. AP600 design certification thermal hydraulics testing and analysis

    International Nuclear Information System (INIS)

    Westinghouse Electric Corporation, in conjunction with the Department of Energy and the Electric Power Research Institute, have been developing an advanced light water reactor design; the AP600. The AP600 is a 1940 Mwt, 600Mwe unit which is similar to a Westinghouse two-loop Pressurized Water Reactor. The accumulated knowledge on reactor design to reduce the capital costs, construction time, and the operational and maintenance cost of the unit once it begins to generate electrical power. The AP600 design goal is to maintain an overall cost advantage over fossil generated electrical power

  8. AP600 design certification thermal hydraulics testing and analysis

    Energy Technology Data Exchange (ETDEWEB)

    Hochreiter, L.E.; Piplica, E.J.

    1995-09-01

    Westinghouse Electric Corporation, in conjunction with the Department of Energy and the Electric Power Research Institute, have been developing an advanced light water reactor design; the AP600. The AP600 is a 1940 Mwt, 600Mwe unit which is similar to a Westinghouse two-loop Pressurized Water Reactor. The accumulated knowledge on reactor design to reduce the capital costs, construction time, and the operational and maintenance cost of the unit once it begins to generate electrical power. The AP600 design goal is to maintain an overall cost advantage over fossil generated electrical power.

  9. A new gap separation mechanism for APS insertion devices.

    Energy Technology Data Exchange (ETDEWEB)

    Trakhtenberg, E. M.; Tcheskidov, V.; Den Hartog, P. K.; Deriy, B.; Erdmann, M.; Makarov, O.; Moog, E. R.

    1999-10-25

    A new gap separation mechanism for use with the standard Advanced Photon Source (APS) 3.3-cm-period undulator magnetic structures has been designed and built and the first system has been installed in the APS storage ring. The system allows a minimum magnetic gap of 10 mm for use with the APS 8-mm insertion device vacuum chambers. The mechanism is a bolted steel frame structure with a simple 4-motor mechanical drive train. The control system uses servomotors with incremental rotary encoders and virtual absolute linear encoders.

  10. Analysis of an AP600 intermediate-size loss-of-coolant accident

    Energy Technology Data Exchange (ETDEWEB)

    Boyack, B.E.; Lime, J.F. [Los Alamos National Lab., NM (United States)

    1995-09-01

    A postulated double-ended guillotine break of an AP600 direct-vessel-injection line has been analyzed. This event is characterized as an intermediate-break loss-of-coolant accident. Most of the insights regarding the response of the AP600 safety systems to the postulated accident are derived from calculations preformed with the TRAC-PF1/MOD2 code. However, complementary insights derived from a scaled experiment conducted in the ROSA facility, as well as insights based upon calculations by other codes, are also presented. Based upon the calculated and experimental results, the AP600 will not experience a core heat up and will reach a safe shutdown state using only safety-class equipment. Only the early part of the long-term cooling period initiated by In-containment Refueling Water Storage Tank injection was evaluated. Thus, the observation that the core is continuously cooled should be verified for the later phase of the long-term cooling period when sump injection and containment cooling processes are important.

  11. Dicty_cDB: FC-AP17 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 4.1 SJMBIB09 SJM Schistosoma japonicum cDNA, mRNA sequence. 80 8e-20 2 BE859184 |BE859184.1 SsS0499 Suaeda salsa ZAP...FC (Link to library) FC-AP17 (Link to dictyBase) - - - Contig-U16254-1 FC-AP17Z (Li...nk to Original site) - - FC-AP17Z 546 - - - - Show FC-AP17 Library FC (Link to library) Clone ID FC-AP17 (Li.../dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP17Q.Seq.d/ Representative seq. ID FC-AP...17Z (Link to Original site) Representative DNA sequence >FC-AP17 (FC-AP17Q) /CSM/FC/FC-AP/FC-AP17Q.Seq.

  12. Dicty_cDB: FC-AP11 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available A clone IMAGE:4920557 5', mRNA sequence. 56 1e-04 2 BF345929 |BF345929.1 602017931F1 NCI_CGAP_Brn67 Homo sap...FC (Link to library) FC-AP11 (Link to dictyBase) - - - Contig-U16101-1 FC-AP11F (Li...nk to Original site) FC-AP11F 394 - - - - - - Show FC-AP11 Library FC (Link to library) Clone ID FC-AP11 (Li.../dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP11Q.Seq.d/ Representative seq. ID FC-AP...11F (Link to Original site) Representative DNA sequence >FC-AP11 (FC-AP11Q) /CSM/FC/FC-AP/FC-AP11Q.Seq.

  13. Dicty_cDB: FC-AP18 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP18 (Link to dictyBase) - - - Contig-U16455-1 FC-AP18Z (Li...nk to Original site) - - FC-AP18Z 367 - - - - Show FC-AP18 Library FC (Link to library) Clone ID FC-AP18 (Li.../dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP18Q.Seq.d/ Representative seq. ID FC-AP...18Z (Link to Original site) Representative DNA sequence >FC-AP18 (FC-AP18Q) /CSM/FC/FC-AP/FC-AP18Q.Seq....XELTPSRPMCVESFNEYPP LGRFAVRDMGQTVAVGVIKSTVKKAPGKAGDKKGAXAPSKKK*innis**iafynnfkkk kkkkk Translated Amino Acid

  14. Dicty_cDB: FC-AP16 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available FC (Link to library) FC-AP16 (Link to dictyBase) - - - Contig-U16269-1 FC-AP16Z (Li...nk to Original site) - - FC-AP16Z 552 - - - - Show FC-AP16 Library FC (Link to library) Clone ID FC-AP16 (Li.../dictycdb.biol.tsukuba.ac.jp/CSM/FC/FC-AP/FC-AP16Q.Seq.d/ Representative seq. ID FC-AP...16Z (Link to Original site) Representative DNA sequence >FC-AP16 (FC-AP16Q) /CSM/FC/FC-AP/FC-AP16Q.Seq....7. 3.28 Translated Amino Acid sequence ---RNRRYKVRKGPLVVVSGKTTVSQALRNIPGVEVANVSRLNLLKLAPGGHLGRFIIWT KSAFEQLD

  15. Comparative evaluation of transport mechanisms of trans-1-amino-3-[¹⁸F]fluorocyclobutanecarboxylic acid and L-[methyl-¹¹C]methionine in human glioma cell lines.

    Science.gov (United States)

    Ono, Masahiro; Oka, Shuntaro; Okudaira, Hiroyuki; Schuster, David M; Goodman, Mark M; Kawai, Keiichi; Shirakami, Yoshifumi

    2013-10-16

    Positron emission tomography (PET) with amino acid tracers is useful for the visualization and assessment of therapeutic effects on gliomas. Our purpose is to elucidate the transport mechanisms of trans-1-amino-3-[¹⁸F]fluorocyclobutanecarboxylic acid (anti-[¹⁸F]FACBC) and L-[methyl-¹¹C]methionine ([¹¹C]Met) in normal human astrocytes (NHA), low-grade (Hs683, SW1088), and high-grade (U87MG, T98G) human glioma cell lines. Because the short half-lives of fluorine-18 and carbon-11 are inconvenient for in vitro experiments, trans-1-amino-3-fluoro[1-¹⁴C]cyclobutanecarboxylic acid (anti-[¹⁴C]FACBC) and L-[methyl-¹⁴C]methionine ([¹⁴C]Met) were used instead of the PET tracers. Time-course uptake experiments showed that uptake of anti-[¹⁴C]FACBC was 1.4-2.6 times higher than that of [¹⁴C]Met in NHA and low-grade glioma cells, and was almost equal to that of [¹⁴C]Met in high-grade glioma cells. To identify the amino acid transporters (AATs) involved in the transport of anti-[¹⁴C]FACBC and [¹⁴C]Met, we carried out competitive inhibition experiments using synthetic/naturally-occurring amino acids as inhibitors. We found that anti-[¹⁴C]FACBC uptake in the presence of Na⁺ was strongly inhibited by L-glutamine and L-serine (the substrates for ASC system AATs), whereas L-phenylalanine and 2-amino-bicyclo[2,2,1]heptane-2-carboxylic acid (BCH, the substrates for L system AATs) robustly inhibited Na⁺-independent anti-[¹⁴C]FACBC uptake. Regardless of Na⁺, [¹⁴C]Met uptake was inhibited strongly by L-phenylalanine and BCH. Moreover, the exchange transport activity of L-glutamine for anti-[¹⁴C]FACBC was stronger than that of BCH in the presence of Na⁺, whereas that for [¹⁴C]Met was almost equal to BCH. These results demonstrate that ASC and L are important transport systems for anti-[¹⁸F]FACBC uptake, while system L is predominantly involved in [¹¹C]Met transport in human astrocytes and glioma cells.

  16. Heavy transport problems

    International Nuclear Information System (INIS)

    Assuming that very often a long transport route from the factory of the manufacturer to the provided site has to be reckoned with, in general only transport with a ship is possible. As each site is only called by a certain steamship line, at a very early stage of planning the nuclear power plant the possibilities and capacities of the lines and means of transportation under discussion should be investigated. In planning the unloading equipment at the site, due consideration should be given to the fact that at a later time this equipment should also be suitable for the transport of heavy components and spent fuel assemblies. (orig.)

  17. Tank characterization report for double shell tank 241-AP-104

    Energy Technology Data Exchange (ETDEWEB)

    Winkelman, W.D., Westinghouse Hanford

    1996-08-07

    This document summarizes the information on the historical uses, present status, and the sampling and analysis results of waste stored in Tank 241-AP-104. This report supports the requirements of Tri-Party Agreement Milestone M-44-09.

  18. Lumbar pedicle screw placement: Using only AP plane imaging

    Directory of Open Access Journals (Sweden)

    Anil Sethi

    2012-01-01

    Conclusion: Placement of pedicle screws under fluoroscopic guidance using AP plane imaging alone with tactile guidance is safe, fast, and reliable. However, a good understanding of the radiographic landmarks is a prerequisite.

  19. ApúnteleaBogotá.com

    OpenAIRE

    2012-01-01

    Se contempla la constitución de una empresa de servicios bajo la razón social apunteleabogota.com, diseñada para facilitar a los visitantes que por motivo de capacitación o negocios deben viajar a la ciudad de Bogotá. apunteleabogota.com tiene como objetivo ofrecer a ejecutivos, directivos de empresas, estudiantes, entre otros una guía de servicios que cubran las necesidades de transporte, alojamiento, infraestructura y estadía, durante la permanencia en la ciudad, por motivos empresariales o...

  20. Roles of AP-2 in clathrin-mediated endocytosis.

    Directory of Open Access Journals (Sweden)

    Emmanuel Boucrot

    Full Text Available BACKGROUND: The notion that AP-2 clathrin adaptor is an essential component of an endocytic clathrin coat appears to conflict with recent observations that substantial AP-2 depletion, using RNA interference with synthesis of AP-2 subunits, fails to block uptake of certain ligands known to internalize through a clathrin-based pathway. METHODOLOGY/PRINCIPAL FINDINGS: We report here the use of in vivo imaging data obtained by spinning-disk confocal microscopy to study the formation of clathrin-coated structures at the plasma membranes of BSC1 and HeLa cells depleted by RNAi of the clathrin adaptor, AP-2. Very few clathrin coats continue to assemble after AP-2 knockdown. Moreover, there is a total absence of clathrin-containing structures completely lacking AP-2 while all the remaining coats still contain a small amount of AP-2. These observations suggest that AP-2 is essential for endocytic coated-pit and coated-vesicle formation. We also find that AP-2 knockdown strongly inhibits light-density lipoprotein (LDL receptor-mediated endocytosis, as long as cells are maintained in complete serum and at 37 degrees C. If cells are first incubated with LDL at 4 degrees C, followed by warming, there is little or no decrease in LDL uptake with respect to control cells. LDL uptake at 37 degrees C is also not affected in AP-2 depleted cells first deprived of LDL by incubation with either serum-starved or LDL-starved cells for 24 hr. The LDL-deprived cells display a significant increase in endocytic structures enriched on deeply invaginated tubes that contain LDL and we suggest that under this condition of stress, LDL might enter through this alternative pathway. CONCLUSIONS/SIGNIFICANCE: These results suggest that AP-2 is essential for endocytic clathrin coated-pit and coated-vesicle formation. They also indicate that under normal conditions, functional endocytic clathrin coated pits are required for LDL internalization. We also show that under certain

  1. Physical Characteristics Preliminary Analysis on AP1000 Fuel Assembly%AP1000典型燃料组件物理特性初步分析

    Institute of Scientific and Technical Information of China (English)

    曹博; 陈义学; 李晓静; 张顺; 张斌; 余慧

    2013-01-01

    AP1000堆芯首循环装料采用5种富集度的组件,使用IFBA、WABA可燃毒物,且部分组件两端设有轴向再生区,堆芯布置复杂。本文采用SCALE程序系统中三维蒙特卡罗输运程序KENO及二维离散纵标程序NEWT ,进行了典型燃料组件的栅格物理计算。研究了深燃耗下不同富集度栅元、燃料组件无限增殖因子(kinf )及主要可燃耗核素核子密度随燃耗的变化规律,得到了A P1000典型燃料组件深燃耗物理特性。%In the first cycle loading of the AP1000 reactor ,assemblies with five kinds of enrichments and burnable poisons such as IFBA , WABA are employed . For some assemblies ,there are regeneration blankets at both ends . All these make the core arrangement extremely complex .The lattice physics calculation was performed using the KENO 3-D Monte Carlo criticality computer code and NEWT multigroup discrete ordinates radiation transport code of SCALE code system .The kinf and atomic number density of typical isotopes as a function of burnup for different enrichment fuel cells and fuel assemblies were studied on the condition of the deep burnup , and the physical characteristic of AP1000 assembly was concluded .

  2. AP-102/104 Retrieval control system qualification test procedure

    Energy Technology Data Exchange (ETDEWEB)

    RIECK, C.A.

    1999-05-18

    This Qualification Test Procedure documents the results of the qualification testing that was performed on the Project W-211, ''Initial Tank Retrieval Systems,'' retrieval control system (RCS) for tanks 241-AP-102 and 241-AP-104. The results confirm that the RCS has been programmed correctly and that the two related hardware enclosures have been assembled in accordance with the design documents.

  3. AP1000 design adaptation to European Markets and EUR compliance

    International Nuclear Information System (INIS)

    The AP1000 plant is an 1100-MWe pressurized water reactor (PWR) with passive safety features and extensive plant simplifications that enhance construction, operation, maintenance and safety. One of the key approaches in the AP1000 design is to use passive features to mitigate design basis accidents. In addition to redundancy, these features incorporate diversity based on PRA insights. The AP1000 design has incorporated a standardization approach, which together with the level of safety achieved by the passive safety features, results in a plant design that can be applied to different geographical regions with varying regulatory standards and utility expectations without major changes. Westinghouse has cooperated for almost 2 decades with European utilities to ensure adaptation of the AP1000 design to European market. The AP1000 50 Hz standard design (also referred to as European Passive Standard) is the resulting adaptation of the AP1000 design to European market needs and requirements, addressing both customer input from the EPP program and regulatory needs identified in the UK GDA process. This standard 50 Hz design retains the overall AP1000 design (safe, simple, standard), the use of proven components and its cost, safety and operability advantages, while it incorporates some changes to adapt to the European environment. In this paper, the key changes incorporated into the AP1000 50 Hz design are discussed, including: -) Adaptation of the electrical systems and Turbine adaptation from 60 Hz to 50 Hz, including approach to IEC vs IEEE compliance; -) Modifications to defense in depth (US class D, European Class 2) and other systems to simplify European licensing and address customer requirements; -) Option for adoption of KSB RUV type Main Reactor Coolant Pumps; -) Limited design adaptations in the areas of instrumentation-control and ventilation and filtration to adapt to European requirements and simplify the licensing process

  4. Analysis and characterization of double shell tank 241-AP-108

    International Nuclear Information System (INIS)

    This document is the first part of a three-part report describing the analysis and characterization of double shell tank 241-AP-108 which is located at the Hanford Reservation.This document is the analytical laboratory data package entitled 'Analysis and Characterization of Double Shell Tank 241-AP-108' which contains a case sampling history, the sampling protocols, the analytical procedures, sampling and analysis quality assurance and quality control measures, and chemical analysis results for samples obtained from the tank

  5. The Dushak–Erekdag Survey of roAp Stars

    Indian Academy of Sciences (India)

    Tatyana Dorokhova; Nikolay Dorokhov

    2005-06-01

    The search of roAp stars at Mt. Dushak–Erekdag Observatory was started in 1992 using the 0.8m Odessa telescope equipped with a two-star high-speed photometer. We have observed more than a dozen stars so far and discovered HD 99563 as roAp star while BD+8087 is suspected to have rapid oscillations. Negative results of our observations for the search of rapid oscillations in four stars in NGC 752 are also discussed.

  6. 49 CFR 192.707 - Line markers for mains and transmission lines.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Line markers for mains and transmission lines. 192... § 192.707 Line markers for mains and transmission lines. (a) Buried pipelines. Except as provided in... buried main and transmission line: (1) At each crossing of a public road and railroad; and (2)...

  7. Ectopic expression of a grape aspartic protease gene, AP13, in Arabidopsis thaliana improves resistance to powdery mildew but increases susceptibility to Botrytis cinerea.

    Science.gov (United States)

    Guo, Rongrong; Tu, Mingxing; Wang, Xianhang; Zhao, Jiao; Wan, Ran; Li, Zhi; Wang, Yuejin; Wang, Xiping

    2016-07-01

    The grape aspartic protease gene, AP13 was previously reported to be responsive, in Chinese wild Vitis quinquangularis cv. 'Shang-24', to infection by Erysiphe necator, the causal agent of powdery mildew disease, as well as to treatment with salicylic acid in V. labrusca×V. vinifera cv. 'Kyoho'. In the current study, we evaluated the expression levels of AP13 in 'Shang-24' in response to salicylic acid (SA), methyl jasmonate (MeJA) and ethylene (ET) treatments, as well as to infection by the necrotrophic fungus, Botrytis cinerea, and the transcript levels of VqAP13 decreased after B. cinerea infection and MeJA treatment, but increased following ET and SA treatments. Transgenic Arabidopsis thaliana lines over-expressing VqAP13 under the control of a constitutive promoter showed enhanced resistance to powdery mildew and to the bacterium Pseudomonas syringae pv. tomato DC3000, and accumulated more callose than wild type plants, while the resistance of transgenic A. thaliana lines to B. cinerea inoculation was reduced. In addition, the expression profiles of various disease resistance- related genes in the transgenic A. thaliana lines following infection by different pathogens were compared to the equivalent profiles in the wild type plants. The results suggest that VqAP13 action promotes the SA dependent signal transduction pathway, but suppresses the JA signal transduction pathway. PMID:27181943

  8. C. Petrone et al.: "Magnetic measurement of the model magnet QD0 designed for the CLIC final focus beam transport line." CERN TE-MSC Internal Note, EDMS Nr: 1184196

    CERN Document Server

    Arpaia, Pasquale; Petrone, Carlo; Russenschuck, Stephan; Walckiers, Louis

    2012-01-01

    This note presents the results of the magnetic measurements performed on QD0, model magnet for the final focus transport line for CLIC (Fig. 1). This high-gradient, hybrid quadrupole has a yoke length of 0.1 m and an aperture of 8.3 mm. ND2Fe14B Permanent magnet blocks provide a gradient of 150 T/m, which can be further increased to 530 T/m when the four coils are excited to 18.3 A. The request was to measure the strength of the field and the multipole coefficients at different currents. The measurement of the field strength, by means of the single stretched wire system, was done in December 2011 in the I8 laboratory. The measurement of the multipole was done by means of the oscillating wire system [1][2].

  9. Parthenolide inhibits ERK and AP-1 which are dysregulated and contribute to excessive IL-8 expression and secretion in cystic fibrosis cells

    Directory of Open Access Journals (Sweden)

    Saadane Aicha

    2011-10-01

    Full Text Available Abstract Background Excessive secretion of IL-8 characterizes cystic fibrosis (CF. This has been attributed to excessive activation of epithelial cell I-κB Kinase and/or NFκB. Maximum IL-8 production requires 3 cooperative mechanisms: 1 release of the promoter from repression; 2 activation of transcription by NFκB and AP-1; 3 stabilization of mRNA by p38-MAPK. Little is known about regulation of IL-8 by MAPKs or AP-1 in CF. Methods We studied our hypothesis in vitro using 3-cellular models. Two of these models are transformed cell lines with defective versus normal cystic fibrosis transmembrane conductance regulator (CFTR expression: an antisense/sense transfected cell line and the patient derived IB3-1/S9. In the third series of studies, we studied primary necropsy human tracheal epithelial cells treated with an inhibitor of CFTR function. All cell lines were pretreated with parthenolide and then stimulated with TNFα and/or IL-1β. Results In response to stimulation with TNFα and/or IL-1β, IL-8 production and mRNA expression was greater in CF-type cells than in non-CF controls. This was associated with enhanced phosphorylation of p38, ERK1/2 and JNK and increased activation of AP-1. Since we previously showed that parthenolide inhibits excessive IL-8 production by CF cells, we evaluated its effects on MAPK and AP-1 activation and showed that parthenolide inhibited ERK and AP-1 activation. Using a luciferase promoter assay, our studies showed that parthenolide decreased activation of the IL-8 promoter in CF cells stimulated with TNFα/IL-1β. Conclusions In addition to NFκB MAPKs ERK, JNK and p38 and the transcription factor AP-1 are also dysregulated in CF epithelial cells. Parthenolide inhibited both NFκB and MAPK/AP-1 pathways contributing to the inhibition of IL-8 production.

  10. Mapping of the spontaneous deletion in the Ap3d1 gene of mocha mice: fast and reliable genotyping

    Directory of Open Access Journals (Sweden)

    Delenclos Marion

    2008-11-01

    Full Text Available Abstract Background The mocha mouse carries a spontaneous deletion in the Ap3d1 gene, encoding the delta 1 subunit of the adaptor related protein complex 3, (Ap3d1, and subsequently lack the expression of functional AP-3. This leads to a deficiency in vesicle transport and storage, which affects neurotransmitter vesicle turnover and release in the central nervous system. Since the genomic sequence of the Ap3d1 gene of mocha mouse is not known, precise mapping of the deletion as well as reliable genotyping protocols are lacking. Findings We sequenced the Ap3d1 gene (HGNC GeneID: 8943 around the deletion site in the mocha mouse and revealed a 10639 bp deletion covering exon 2 to 6. Subsequently, new PCR primers were designed yielding a reliable genotyping protocol of both newborn and adult tissue. To examine the genotypes further, hippocampal neurons were cultured from mocha and control mice. Patch-clamp recordings showed that mocha neurons had a higher input resistance, and that autaptic EPSC in mocha cultures depressed faster and stronger as compared with control cultures. Conclusion Our study reports the sequence of the deleted part of the Ap3d1 gene in mocha mice, as well as a reliable PCR-based genotyping protocol. We cultured hippocampal neurons from control and mocha mice, and found a difference in input resistance of the neurons, and in the synaptic short-term plasticity of glutamatergic autapses showing a larger synaptic depression than controls. The described procedures may be useful for the future utilization of the mocha mouse as a model of defective vesicle biogenesis. Importantly, as genotyping by eye color is complicated in newborn mice, the designed protocol is so fast and reliable that newborn mice could rapidly be genotyped and hippocampal neurons dissociated and cultured, which is normally best done at P0-P2.

  11. APS storage ring vacuum system development

    International Nuclear Information System (INIS)

    The Advanced Photon Source synchrotron radiation facility, under construction at the Argonne National Laboratory, incorporates a large ring for the storage of 7 GeV positrons for the generation of photon beams for the facility's materials research program. The Storage Ring's 1104 m circumference is divided into 40 sectors which contain vacuum, beam transport, control, rf and insertion device systems. The vacuum system will operate at a pressure of 1 nTorr and is fabricated from aluminum. The system includes distributed NeG pumping, photon absorbers with lumped pumping, beam position monitors, vacuum diagnostics and valving. An overview of the vacuum system design and details of selected development program results are presented. 5 refs

  12. An AP4B1 frameshift mutation in siblings with intellectual disability and spastic tetraplegia further delineates the AP-4 deficiency syndrome.

    Science.gov (United States)

    Abdollahpour, Hengameh; Alawi, Malik; Kortüm, Fanny; Beckstette, Michael; Seemanova, Eva; Komárek, Vladimír; Rosenberger, Georg; Kutsche, Kerstin

    2015-02-01

    The recently proposed adaptor protein 4 (AP-4) deficiency syndrome comprises a group of congenital neurological disorders characterized by severe intellectual disability (ID), delayed or absent speech, hereditary spastic paraplegia, and growth retardation. AP-4 is a heterotetrameric protein complex with important functions in vesicle trafficking. Mutations in genes affecting different subunits of AP-4, including AP4B1, AP4E1, AP4S1, and AP4M1, have been reported in patients with the AP-4 deficiency phenotype. We describe two siblings from a non-consanguineous couple who presented with severe ID, absent speech, microcephaly, growth retardation, and progressive spastic tetraplegia. Whole-exome sequencing in the two patients identified the novel homozygous 2-bp deletion c.1160_1161delCA (p.(Thr387Argfs*30)) in AP4B1. Sanger sequencing confirmed the mutation in the siblings and revealed it in the heterozygous state in both parents. The AP4B1-associated phenotype has previously been assigned to spastic paraplegia-47. Identification of a novel AP4B1 alteration in two patients with clinical manifestations highly similar to other individuals with mutations affecting one of the four AP-4 subunits further supports the observation that loss of AP-4 assembly or functionality underlies the common clinical features in these patients and underscores the existence of the clinically recognizable AP-4 deficiency syndrome.

  13. DNA cleavage at the AP site via β-elimination mediated by the AP site-binding ligands.

    Science.gov (United States)

    Abe, Yukiko S; Sasaki, Shigeki

    2016-02-15

    DNA is continuously damaged by endogenous and exogenous factors such as oxidation and alkylation. In the base excision repair pathway, the damaged nucleobases are removed by DNA N-glycosylase to form the abasic sites (AP sites). The alkylating antitumor agent exhibits cytotoxicity through the formation of the AP site. Therefore blockage or modulation of the AP site repair pathway may enhance the antitumor efficacy of DNA alkylating agents. In this study, we have examined the effects of the nucleobase-polyamine conjugated ligands (G-, A-, C- and T-ligands) on the cleavage of the AP site. The G- and A-ligands cleaved DNA at the AP site by promoting β-elimination in a non-selective manner by the G-ligand, and in a selective manner for the opposing dT by the A-ligand. These results suggest that the nucleobase-polyamine conjugate ligands may have the potential for enhancement of the cytotoxicities of the AP site.

  14. 丝绸之路经济带物流运输线路BIM标准体系建设%The BIM standard system construction of Silk Road Economic Zone logistics transportation line

    Institute of Scientific and Technical Information of China (English)

    何斌; 赵晓文; 柯谱; 纳曼·麦麦提

    2015-01-01

    针对“丝绸之路经济带”物流线路体系建设的难点,在分析BIM基本含义的基础上,提出了物流运输线路BIM标准体系的理念,并介绍了该体系的组成内容,探讨了物流系统BIM标准与GIS系统融合的必要性,可有效提高物流系统的管理效益和水平。%According to the problems of“Silk Road Economic Zone”logistics line system construction,based on analyzing the basis implication of BIM,put forward the concept of logistics transportation line standard system,and introduced the contents of the system,discussed the necessity of logistics system BIM standard and GIS system fusion,could effectively improve the management efficiency and level of logistics system.

  15. Authenticated, private, and secured smart cards (APS-SC)

    Science.gov (United States)

    Szu, Harold; Mehmood, Amir

    2006-04-01

    From historical perspective, the recent advancements in better antenna designs, low power circuitry integrations and inexpensive fabrication materials have made possible a miniature counter-measure against Radar, a clutter behaving like a fake target return called Digital Reflection Frequency Modulation (DRFM). Such a military counter-measure have found its way in the commerce as a near field communication known as Radio Frequency Identification (RFID), a passive or active item tag T attached to every readable-writable Smart Card (SC): Passports ID, medical patient ID, biometric ID, driver licenses, book ID, library ID, etc. These avalanche phenomena may be due to the 3 rd Gen phones seeking much more versatile & inexpensive interfaces, than the line-of-sight bar-code optical scan. Despite of the popularity of RFID, the lacking of Authenticity, Privacy and Security (APS) protection restricted somewhat the wide spread commercial, financial, medical, legal, and militarily applications. Conventional APS approach can obfuscate a private passkey K of SC with the tag number T or the reader number R, or both, i.e. only T*K or R*K or both will appear on them, where * denotes an invertible operation, e.g. EXOR, but not limited to it. Then, only the authentic owner, knowing all, can inverse the operation, e.g. EXOR*EXOR= I to find K. However, such an encryption could be easily compromised by a hacker seeking exhaustively by comparison based on those frequently used words. Nevertheless, knowing biological wetware lesson for power of pairs sensors and Radar hardware counter-measure history, we can counter the counter-measure DRFM, instead using one RFID tag per SD, we follow the Nature adopting two ears/tags, e.g. each one holding portions of the ID or simply two different ID's readable only by different modes of the interrogating reader, followed by brain central processor in terms of nonlinear invertible shufflers mixing two ID bits. We prefer to adopt such a hardware

  16. Enigma interacts with adaptor protein with PH and SH2 domains to control insulin-induced actin cytoskeleton remodeling and glucose transporter 4 translocation

    DEFF Research Database (Denmark)

    Barres, Romain; Grémeaux, Thierry; Gual, Philippe;

    2006-01-01

    APS (adaptor protein with PH and SH2 domains) initiates a phosphatidylinositol 3-kinase-independent pathway involved in insulin-stimulated glucose transport. We recently identified Enigma, a PDZ and LIM domain-containing protein, as a partner of APS and showed that APS-Enigma complex plays...

  17. Validation of the new trapped environment AE9/AP9/SPM at low Earth orbit

    Science.gov (United States)

    Badavi, Francis F.

    2014-09-01

    The completion of the international space station (ISS) in 2011 has provided the space research community an ideal proving ground for future long duration human activities in space. Ionizing radiation measurements in ISS form the ideal tool for the validation of radiation environmental models, nuclear transport codes and nuclear reaction cross sections. Indeed, prior measurements on the space transportation system (STS; shuttle) provided vital information impacting both the environmental models and the nuclear transport code developments by indicating the need for an improved dynamic model of the low Earth orbit (LEO) trapped environment. Additional studies using thermo-luminescent detector (TLD), tissue equivalent proportional counter (TEPC) area monitors, and computer aided design (CAD) model of earlier ISS configurations, confirmed STS observations that, as input, computational dosimetry requires an environmental model with dynamic and directional (anisotropic) behavior, as well as an accurate six degree of freedom (DOF) definition of the vehicle attitude and orientation along the orbit of ISS. At LEO, a vehicle encounters exposure from trapped particles and attenuated galactic cosmic rays (GCR). Within the trapped field, a challenge arises from properly estimating the amount of exposure acquired. There exist a number of models to define the intensities of the trapped particles during the solar quiet and active times. At active times, solar energetic particles (SEP) generated by solar flare or coronal mass ejection (CME) also contribute to the exposure at high northern and southern latitudes. Among the more established trapped models are the historic and popular AE8/AP8, dating back to the 1980s, the historic and less popular CRRES electron/proton, dating back to 1990s and the recently released AE9/AP9/SPM. The AE9/AP9/SPM model is a major improvement over the older AE8/AP8 and CRRES models. This model is derived from numerous measurements acquired over four

  18. AP@home: The Artificial Pancreas Is Now at Home.

    Science.gov (United States)

    Heinemann, Lutz; Benesch, Carsten; DeVries, J Hans

    2016-07-01

    In the past years the development of an artificial pancreas (AP) has made great progress and many activities are ongoing in this area of research. The major step forward made in the last years was moving the evaluation of AP systems from highly controlled experimental conditions to daily life conditions at the home of patients with diabetes; this was also the aim of the European Union-funded AP@home project. Over a time period of 5 years a series of clinical studies were performed that culminated in 2 "final studies" during which an AP system was used by patients in their home environment for 2 or 3 months without supervision by a physician, living their normal lives. Two different versions of the AP system developed within this project were evaluated. A significant improvement in glycated hemoglobin was observed during closed-loop conditions despite the fact that during the control period the patients used the best currently available therapeutic option. In addition, a "single-port AP system" was developed within the project that combines continuous glucose monitoring and insulin infusion at a single tissue site. By using such a combined device the patients not only have to carry one less device around, the number of access points through the skin is also reduced from 2 to 1. In summary, close cooperation of 12 European partners, both academic centers and industry, enabled the development and evaluation of AP systems under daily life conditions. The next step is to develop these into products in cooperation with commercial partners. PMID:26888971

  19. 环状铁路在大都市交通中的重要作用——以东京山手线铁路为例%Enlightenment of Yamanote Loop Line in Tokyo: an Important Role of Rail Loop Line in Metropolitan Transportation

    Institute of Scientific and Technical Information of China (English)

    王晓荣; 荣朝和; 盛来芳

    2013-01-01

    东京大都市区公共交通的高效率与以山手环线为核心的铁路网络密切相关.以历史资料与现状数据为基础,介绍了山手线的形成与强化过程,分析了其作为环形通道和环状枢纽体系的功能.主要结论如下:①以山手线为中心的铁路网络对东京交通与城市时空形态产生重要影响,铁路网络形态,特别是其枢纽体系对提高城市时空要素转换效率具有重要意义;②环线铁路有助于形成城市枢纽体系的有效宏观界面;③以北京为例分析了山手线经验对我国城市及铁路规划的启示,主张强化铁路环线的界面效应.%The high efficiency of public transit system in Tokyo metropolitan area is closely related to rail network centering on Yamanote Loop Line. The authors introduced the process of the formation and intensification of the Yamanote Loop Line, analyzed its core function as the loop corridor and loop hub system, as well as its significant impact on the time-space form of Tokyo transportation and Urban Space. The main point of the paper is as follows: (1) rail network form, especially its hub system is important in improving the efficiency of Public transportation and transforming the time-space elements; (2) pointed out the contribution of rail loop line to the formation of effective macro-interface of urban hub system. (3)take Beijing as an example, we analyzed the revelation of Yamanote Loop Line corresponding to China' s cities and railway planning, and proposed that the interface effective of railway circle should be strengthened.

  20. APS DFD 2011 video submission V045

    CERN Document Server

    Swan, David; Ward, Thomas

    2011-01-01

    Inhomogeneous uid mixing in a tilted-rotating cylindrical tank (radius a = 3:5 cm) is shown at Re(17-40) and low capillary numbers. A water and surfactant solu- tion (1% by mass sodium oleate) is dispersed in soybean oil (95% by volume), through varying the rotation rate, and angle of inclination, the rate of mixing is observed. A planar laser is directed down the tank axis to highlight a cross-sectional area of the fluid volume and as the water droplets begin to break up to sizes on the order of the beam width and less, more light is refracted and the mixture is illuminated. Initially, the water breaks up into large droplets that exhibit approximate solid-body rotation about the bottom of the tank. When the total combined volume is below the critical volume of the tank Vcrit = a^3 tan vortex transport of the water occurs more rapidly, breaking up the water into continually smaller droplets in a process that resembles periodic shearing. When the fluid volume is above critical the water will break up and rotat...

  1. 49 CFR 192.377 - Service lines: Copper.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Service lines: Copper. 192.377 Section 192.377 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY... § 192.377 Service lines: Copper. Each copper service line installed within a building must be...

  2. 49 CFR 192.371 - Service lines: Steel.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Service lines: Steel. 192.371 Section 192.371 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY... § 192.371 Service lines: Steel. Each steel service line to be operated at less than 100 p.s.i. (689...

  3. AP1000 - the new standard for nuclear power

    International Nuclear Information System (INIS)

    Full text of publication follows: The AP1000 is the only Generation III+ reactor to receive Final Design Approval (FDA) from the Nuclear Regulatory Commission, and is expected to receive its Design Certification by the end of the year. Building on the proven features of current generation nuclear plants, the AP1000 combines experience with innovation into a design that surpasses current standards of safety and reliability. Use of passive safety features results in a simpler and more compact design that enhances safety, simplifies O and M requirements, and reduces capital and operating costs. At 1117 Mwe, the AP1000 is well suited for almost any grid system and will be fully competitive with combined-cycle gas and comparable fossil fuel plants. The AP1000 is ready to help launch a renaissance in new nuclear plant construction throughout the world. Maturity of Design: In excess of 1300 man-years and $400 million in development funding have been expended on the AP1000. It has undergone extensive, part scale testing at the system, sub-system and component level, in addition to a series of part scale integrated tests. The AP1000 is the most analyzed of the next generation reactors. Simplicity of Design/Economics: The AP1000 uses simplified and innovative passive safety systems to an unprecedented extent. Simplified passive safety systems provide reliable operation, reduced capital costs, and enhanced plant safety with large plant operating margins. The AP1000 features improved reliability through simplicity rather than addition of redundant safety trains. This simpler design is easier and less costly to operate and maintain than larger, more complex plants, while less equipment and smaller buildings translate into lower capital costs and shorter construction durations. After construction, economic benefit will be found in reduced operating and maintenance costs, largely due to reduced operating and maintenance staffing requirements. Construction aspects

  4. Stokes IQUV mapping of α2 CVn & other Ap stars using ESPaDOnS and NARVAL

    Science.gov (United States)

    Silvester, J.; Kochukhov, O.; Wade, G. A.

    2013-12-01

    New spectral line polarisation observations of 7 bright Ap stars have been obtained with the ESPaDOnS and Narval high resolution spectropolarimeters (Silvester et al. 2012). The aim of this data set is produce a series of surface magnetic field and surface chemistry maps for these Ap stars. We present new magnetic maps for the Ap star α2 CVn using these new data and the MDI inversion code INVERS10. α2 CVn is the first Ap star to be observed during two separate epochs using high resolution phase resolved spectropolarimetric IQUV observations and as such allows us an insight into how stable the surface magnetic structure is over a decade timescale. We show that the new maps give a magnetic field structure consistent with the previous maps obtained by Kochukhov and Wade (2010) from lower quality MuSiCoS spectra taken a decade ago and that the field topology cannot be described by a dipolar or quadrupolar field.

  5. Stokes IQUV mapping of $\\alpha^2$ CVn & other Ap stars using ESPaDOnS and NARVAL

    CERN Document Server

    Silvester, J; Wade, G A

    2013-01-01

    New spectral line polarisation observations of 7 bright Ap stars have been obtained with the ESPaDOnS and Narval high resolution spectropolarimeters (Silvester et al. 2012). The aim of this data set is produce a series of surface magnetic field and surface chemistry maps for these Ap stars. We present new magnetic maps for the Ap star $\\alpha^2$ CVn using these new data and the MDI inversion code INVERS10. $\\alpha^2$ CVn is the first Ap star to be observed during two separate epochs using high resolution phase resolved spectropolarimetric $IQUV$ observations and as such allows us an insight into how stable the surface magnetic structure is over a decade timescale. We show that the new maps give a magnetic field structure consistent with the previous maps obtained by Kochukhov and Wade (2010) from lower quality MuSiCoS spectra taken a decade ago and that the field topology cannot be described by a dipolar or quadrupolar field.

  6. Mobile docking of REMUS-100 equipped with USBL-APS to an unmanned surface vehicle: A performance feasibility study

    Science.gov (United States)

    Miranda, Mario, II

    The overall objective of this work is to evaluate the ability of homing and docking an unmanned underwater vehicle (Hydroid REMUS 100 UUV) to a moving unmanned surface vehicle (Wave-Adaptive Modular Surface Vehicle USV) using a Hydroid Digital Ultra-Short Baseline (DUSBL) acoustic positioning system (APS), as a primary navigation source. An understanding of how the UUV can rendezvous with a stationary USV first is presented, then followed by a moving USV. Inherently, the DUSBL-APS is susceptible to error due to the physical phenomena of the underwater acoustic channel (e.g. ambient noise, attenuation and ray refraction). The development of an APS model has allowed the authors to forecast the UUV's position and the estimated track line of the USV as determined by the DUSBL acoustic sensor. In this model, focus is placed on three main elements: 1) the acoustic channel and sound ray refraction when propagating in an inhomogeneous medium; 2) the detection component of an ideal DUSBL-APS using the Neyman-Pearson criterion; 3) the signal-to-noise ratio (SNR) and receiver directivity impact on position estimation. The simulation tool is compared against actual open water homing results in terms of the estimated source position between the simulated and the actual USBL range and bearing information.

  7. Past, Present, and Future of AP Chemistry: A Brief History of Course and Exam Alignment Efforts

    Science.gov (United States)

    Magrogan, Serena

    2014-01-01

    As part of the Advanced Placement (AP) Program's commitment to continually enhance alignment with current best practices in college-level learning, the AP Program is currently evaluating and redesigning courses and exams, one of which launched during the 2013-2014 academic school year: AP chemistry. The history of the AP chemistry course and…

  8. AP Report to the Nation: A Closer Look at the Nation and Florida

    Science.gov (United States)

    Sawtell, Ellen A.; Gillie, Jacqueline M.; Smith, Patricia Z.

    2012-01-01

    In February 2012, the College Board published The 8th Annual AP Report to the Nation. This session provides a deeper dive into key information for the United States with an emphasis on Florida, and participants hear how one school in Florida utilizes AP Potential™ to help build their AP Program. Participants also learn about AP participation and…

  9. Vibration analysis of primary air pump JE01 AP003

    International Nuclear Information System (INIS)

    JE01 AP003 primary coolant pump is one of the main components in the safe operation of the reactor so that needs to be done early detection of damage types and levels of its constituent components. The main components of the pump that is often damaged bearing. The purpose of this paper is to know the symptoms of bearing damage and the level of damage based on vibration analysis. One indication of the bearing has been damaged, that is the vibration or temperature rise in the bearing. One way to detect early symptoms of damage to the pump is to use the vibration response peak Based on the analysis of the measurement results cited the obtained results that the primary coolant pump JE01 AP003 has been damage to a free end looseness bearing with moderate levels of damage. Need JE01 AP003 bearing replacement even though the damage is not severe. (author)

  10. Experience with the EPICS PV Gateway at the APS

    CERN Document Server

    Evans, Kenneth

    2005-01-01

    The EPICS PV Gateway has become a stable, high-performance application that provides access to process variables while minimizing the impact on critical IOCs and implementing additional access security. The additional access security typically prevents write access but is highly configurable. The Advanced Photon Source (APS) currently uses 40 Gateways running on 11 machines to provide access to the machine network from the offices and for the individual experimental teams. These include reverse Gateways that allow administration of all 40 APS Gateways from a single MEDM screen, even though the Gateways are running on separate networks. This administration includes starting, stopping, making and viewing reports, and viewing and editing access security files. There is one Gateway that provides process variable renaming. This paper provides an overview of the Gateways at the APS and describes the procedures that have been set up to use and administer them.

  11. PKA-activated ApAF–ApC/EBP heterodimer is a key downstream effector of ApCREB and is necessary and sufficient for the consolidation of long-term facilitation

    OpenAIRE

    LEE, Jin-A; Lee, Sue-Hyun; Lee, Changhoon; Chang, Deok-Jin; Lee, Yong; Kim, Hyoung; Cheang, Ye-Hwang; Ko, Hyoung-Gon; Lee, Yong-Seok; Jun, Heejung; Bartsch, Dusan; Kandel, Eric R.; Kaang, Bong-Kiun

    2006-01-01

    Long-term memory requires transcriptional regulation by a combination of positive and negative transcription factors. Aplysia activating factor (ApAF) is known to be a positive transcription factor that forms heterodimers with ApC/EBP and ApCREB2. How these heterodimers are regulated and how they participate in the consolidation of long-term facilitation (LTF) has not, however, been characterized. We found that the functional activation of ApAF required phosphorylation of ApAF by PKA on Ser-2...

  12. Transcriptional Regulation of the AP-2α Promoter by BTEB-1 and AP-2rep, a Novel wt-1/egr-Related Zinc Finger Repressor

    OpenAIRE

    Imhof, Axel; Schuierer, Marion; Werner, Oliver; Moser, Markus; Roth, Christina; Bauer, Reinhard; BUETTNER, REINHARD

    1999-01-01

    AP-2 transcription factors have been suggested to exert key regulatory functions in vertebrate embryonic development, in tumorigenicity of various cancer cell types, and in controlling cell cycle and apoptotic effector genes. In this study, we investigated transcriptional regulation of the AP-2α gene promoter mediated by an autoregulatory element (referred to as A32) with a core consensus AP-2 binding site at position −336 relative to the mRNA initiation site. AP-2 and multiple different nucl...

  13. Miniature Autonomous Star Tracker Based on CMOS APS

    OpenAIRE

    Ying, Dong; Zheng, You; Fei, Xing; Qin, Chou

    2006-01-01

    An APS (Active Pixel Sensor) based autonomous star tracker (AAST) has been proposed for small satellite and microsatellite attitude determination. A prototype of AAST has been developed. It has low mass of 1kg, low power of 3W, high rate up to 5Hz, and acquisition success rate of higher than 99.9%. The improvements in dimensions and performances are realized with the replacement of the CCD (Charge Coupled Device) by the APS. The optical design of the prototype is based on PSF (Point Spread Fu...

  14. SN 2002ap, the hypernova of class Ic

    OpenAIRE

    Pandey, S. B.; Sahu, D. K.; Anupama, G. C.; Bhattacharya, D; Sagar, Ram

    2003-01-01

    The supernova SN 2002ap was discovered in the outer regions of the nearby spiral M74 on January 29.4 UT. Early photometric and spectroscopic observations indicate the supernova belongs to the class of Ic hypernova. Late time (After JD 2452500) light curve decay slopes are similar to that of the hypernovae SN 1997ef and SN 1998bw. We present here the $BVRI$ photometric light curves and colour evolutions of SN 2002ap to investigate the late time nature of the light curve.

  15. Exploring Mars: The Ares Payload Service (APS)

    Science.gov (United States)

    Bowen, Justin; Lusignan, Bruce

    1999-08-01

    design notably an improved re-entry cooling system and fueling stability analysis were done this year. More technical detail and a proposed international consortium to develop the SSTO is presented in another session of this year's Mars convention. We believe that there will be no human exploration of Mars based on the Shuttle or Expendable launch vehicles, and no resources available except for a cooperative international program. However, just as the world is learning to cooperate in peacekeeping, we hold out the hope that similar cooperation will develop for Mars exploration. With that in mind, this year we asked the question- "How will the human mission get to Mars if it has to use the SsTO for transportation?"

  16. Increased expression of AP2 and Sp1 transcription factors in human thyroid tumors: a role in NIS expression regulation?

    International Nuclear Information System (INIS)

    Sodium/iodide symporter (NIS) is a key protein in iodide transport by thyroid cells and this activity is a prerequisite for effective radioiodide treatment of thyroid cancer. In the majority of thyroid cancers, however, iodide uptake is reduced, probably as a result of decreased NIS protein expression. To identify the mechanisms that negatively affect NIS expression in thyroid tumors, we performed electrophoresis mobility shift assays and immunoblot analysis of nuclear protein extracts from normal and tumoral thyroid tissues from 14 unrelated patients. Two proteins closely related to the transcription factors AP2 and Sp1 were identified in the nuclear extracts. Expression of both AP2 and Sp1 in nuclear extracts from thyroid tumors was significantly higher than that observed in corresponding normal tissues. These observations raise the possibility that NIS expression, and subsequently iodide transport, are reduced in thyroid tumors at least in part owing to alterations in the binding activity of AP2 and Sp1 transcription factors to NIS promoter

  17. Increased expression of AP2 and Sp1 transcription factors in human thyroid tumors: a role in NIS expression regulation?

    Directory of Open Access Journals (Sweden)

    Schlumberger Martin

    2002-12-01

    Full Text Available Abstract Background Sodium/iodide symporter (NIS is a key protein in iodide transport by thyroid cells and this activity is a prerequisite for effective radioiodide treatment of thyroid cancer. In the majority of thyroid cancers, however, iodide uptake is reduced, probably as a result of decreased NIS protein expression. Methods To identify the mechanisms that negatively affect NIS expression in thyroid tumors, we performed electrophoresis mobility shift assays and immunoblot analysis of nuclear protein extracts from normal and tumoral thyroid tissues from 14 unrelated patients. Results Two proteins closely related to the transcription factors AP2 and Sp1 were identified in the nuclear extracts. Expression of both AP2 and Sp1 in nuclear extracts from thyroid tumors was significantly higher than that observed in corresponding normal tissues. Conclusion These observations raise the possibility that NIS expression, and subsequently iodide transport, are reduced in thyroid tumors at least in part owing to alterations in the binding activity of AP2 and Sp1 transcription factors to NIS promoter.

  18. Design of automatic transportation system of negative film production line based on master-slave PLC%基于PLC控制的负极片生产线自动输送系统的设计

    Institute of Scientific and Technical Information of China (English)

    孙贵政; 杨建国; 王庆霞

    2012-01-01

    负极片是高性能能量供给部件之一,负极片生产又是空间电源制造的关键环节.通过对负极片生产线的工作原理和工艺流程的分析,设计了以主-从站PLC和触摸屏为控制核心的负极片生产线自动输送系统.上位机以触摸屏为载体,设计了手动操作及自动运行的界面,下位机的软件系统设计了手动工作方式和自动工作方式的控制系统.该输送系统用四个电机驱动的机械手、两条自动输送线和一个银网放置转盘实现了自动放纸、放模、放网、脱模等物料输送的自动化,有效地提高了负极片生产的效率,避免了有毒物质对生产人员健康的损害.%Negative film is kind of high-performance power supply component negative film production is the key step of space power manufacturing.Through the negative film production line working principle and process analysis , designed a negative film production line automated delivery system based on master-slave PLC and touching screen,achieved materials automatic transportation including paper,mold,silver nets,etc.Effectively improve the efficiency of the negative film production and avoid the toxic substances damaging the health of production personnel.

  19. Incorporating the APS Catalog of the POSS I and Image Archive in ADS

    Science.gov (United States)

    Humphreys, Roberta M.

    1998-01-01

    The primary purpose of this contract was to develop the software to both create and access an on-line database of images from digital scans of the Palomar Sky Survey. This required modifying our DBMS (called Star Base) to create an image database from the actual raw pixel data from the scans. The digitized images are processed into a set of coordinate-reference index and pixel files that are stored in run-length files, thus achieving an efficient lossless compression. For efficiency and ease of referencing, each digitized POSS I plate is then divided into 900 subplates. Our custom DBMS maps each query into the corresponding POSS plate(s) and subplate(s). All images from the appropriate subplates are retrieved from disk with byte-offsets taken from the index files. These are assembled on-the-fly into a GIF image file for browser display, and a FITS format image file for retrieval. The FITS images have a pixel size of 0.33 arcseconds. The FITS header contains astrometric and photometric information. This method keeps the disk requirements manageable while allowing for future improvements. When complete, the APS Image Database will contain over 130 Gb of data. A set of web pages query forms are available on-line, as well as an on-line tutorial and documentation. The database is distributed to the Internet by a high-speed SGI server and a high-bandwidth disk system. URL is http://aps.umn.edu/IDB/. The image database software is written in perl and C and has been compiled on SGI computers with MIX5.3. A copy of the written documentation is included and the software is on the accompanying exabyte tape.

  20. RELATIVISTIC SUPERNOVAE HAVE SHORTER-LIVED CENTRAL ENGINES OR MORE EXTENDED PROGENITORS: THE CASE OF SN 2012ap

    International Nuclear Information System (INIS)

    Deep, late-time X-ray observations of the relativistic, engine-driven, type Ic SN 2012ap allow us to probe the nearby environment of the explosion and reveal the unique properties of relativistic supernova explosions (SNe). We find that on a local scale of ∼0.01 pc the environment was shaped directly by the evolution of the progenitor star with a pre-explosion mass-loss rate of M-dot <5×10−6 M⊙ yr−1, in line with gamma-ray bursts (GRBs) and the other relativistic SN 2009bb. Like sub-energetic GRBs, SN 2012ap is characterized by a bright radio emission and evidence for mildly relativistic ejecta. However, its late-time (δt ≈ 20 days) X-ray emission is ∼100 times fainter than the faintest sub-energetic GRB at the same epoch, with no evidence for late-time central engine activity. These results support theoretical proposals that link relativistic SNe like 2009bb and 2012ap with the weakest observed engine-driven explosions, where the jet barely fails to break out. Furthermore, our observations demonstrate that the difference between relativistic SNe and sub-energetic GRBs is intrinsic and not due to line-of-sight effects. This phenomenology can either be due to an intrinsically shorter-lived engine or to a more extended progenitor in relativistic SNe

  1. Cinnamon extract induces tumor cell death through inhibition of NFκB and AP1

    International Nuclear Information System (INIS)

    Cinnamomum cassia bark is the outer skin of an evergreen tall tree belonging to the family Lauraceae containing several active components such as essential oils (cinnamic aldehyde and cinnamyl aldehyde), tannin, mucus and carbohydrate. They have various biological functions including anti-oxidant, anti-microbial, anti-inflammation, anti-diabetic and anti-tumor activity. Previously, we have reported that anti-cancer effect of cinnamon extracts is associated with modulation of angiogenesis and effector function of CD8+ T cells. In this study, we further identified that anti-tumor effect of cinnamon extracts is also link with enhanced pro-apoptotic activity by inhibiting the activities NFκB and AP1 in mouse melanoma model. Water soluble cinnamon extract was obtained and quality of cinnamon extract was evaluated by HPLC (High Performance Liquid Chromatography) analysis. In this study, we tested anti-tumor activity and elucidated action mechanism of cinnamon extract using various types of tumor cell lines including lymphoma, melanoma, cervix cancer and colorectal cancer in vitro and in vivo mouse melanoma model. Cinnamon extract strongly inhibited tumor cell proliferation in vitro and induced active cell death of tumor cells by up-regulating pro-apoptotic molecules while inhibiting NFκB and AP1 activity and their target genes such as Bcl-2, BcL-xL and survivin. Oral administration of cinnamon extract in melanoma transplantation model significantly inhibited tumor growth with the same mechanism of action observed in vitro. Our study suggests that anti-tumor effect of cinnamon extracts is directly linked with enhanced pro-apoptotic activity and inhibition of NFκB and AP1 activities and their target genes in vitro and in vivo mouse melanoma model. Hence, further elucidation of active components of cinnamon extract could lead to development of potent anti-tumor agent or complementary and alternative medicine for the treatment of diverse cancers

  2. Cinnamon extract induces tumor cell death through inhibition of NFκB and AP1

    Directory of Open Access Journals (Sweden)

    Lee Sung

    2010-07-01

    Full Text Available Abstract Background Cinnamomum cassia bark is the outer skin of an evergreen tall tree belonging to the family Lauraceae containing several active components such as essential oils (cinnamic aldehyde and cinnamyl aldehyde, tannin, mucus and carbohydrate. They have various biological functions including anti-oxidant, anti-microbial, anti-inflammation, anti-diabetic and anti-tumor activity. Previously, we have reported that anti-cancer effect of cinnamon extracts is associated with modulation of angiogenesis and effector function of CD8+ T cells. In this study, we further identified that anti-tumor effect of cinnamon extracts is also link with enhanced pro-apoptotic activity by inhibiting the activities NFκB and AP1 in mouse melanoma model. Methods Water soluble cinnamon extract was obtained and quality of cinnamon extract was evaluated by HPLC (High Performance Liquid Chromatography analysis. In this study, we tested anti-tumor activity and elucidated action mechanism of cinnamon extract using various types of tumor cell lines including lymphoma, melanoma, cervix cancer and colorectal cancer in vitro and in vivo mouse melanoma model. Results Cinnamon extract strongly inhibited tumor cell proliferation in vitro and induced active cell death of tumor cells by up-regulating pro-apoptotic molecules while inhibiting NFκB and AP1 activity and their target genes such as Bcl-2, BcL-xL and survivin. Oral administration of cinnamon extract in melanoma transplantation model significantly inhibited tumor growth with the same mechanism of action observed in vitro. Conclusion Our study suggests that anti-tumor effect of cinnamon extracts is directly linked with enhanced pro-apoptotic activity and inhibition of NFκB and AP1 activities and their target genes in vitro and in vivo mouse melanoma model. Hence, further elucidation of active components of cinnamon extract could lead to development of potent anti-tumor agent or complementary and alternative

  3. Control of machine functions or transport systems

    Energy Technology Data Exchange (ETDEWEB)

    Woodley, M.D.; Lee, M.J.; Jaeger, J.; King, A.S.

    1983-01-01

    A computer code, COMFORT, has been developed at SLAC for on-line calculation of the strengths of magnetic elements in an electron storage ring or transport beam line, subject to first order fitting constraints on the ring or beam line parameters. This code can also be used off-line as an interactive lattice or beam line design tool.

  4. Regulation of amino acid transporters by adenoviral-mediated human insulin-like growth factor-1 in a mouse model of placental insufficiency in vivo and the human trophoblast line BeWo in vitro

    Science.gov (United States)

    Jones, H.; Crombleholme, T.; Habli, M.

    2014-01-01

    Previous work in our laboratory demonstrated that over-expression of human insulin-like growth factor-11 (hIGF-1) in the placenta corrects fetal weight deficits in mouse, rat, and rabbit models of intrauterine growth restriction without changes in placental weight. The underlying mechanisms of this effect have not been elucidated. To investigate the effect of intra-placental IGF-1 over-expression on placental function we examined amino acid transporter expression and localization in both a mouse model of placental Insufficiency (PI) and a model of human trophoblast, the BeWo Choriocarcinoma cell line. For in vitro human studies, BeWo Choriocarcinoma cells were maintained in F12 complete medium + 10%FBS. Cells were incubated in serum-free control media ± Ad-IGF-1 or Ad-LacZ for 48 h. MOIs of 10:1 and 100:1 were utilized. In BeWo, transfection efficiency was 100% at an MOI of 100:1 and Ad-IGF-1 significantly increased IGF-1 secretion, proliferation and invasion but reduced apoptosis compared to controls. In vitro, amino acid uptake was increased following Ad-IGF-1 treatment and associated with significantly increased RNA expression of SNAT1, 2, LAT1 and 4F2hc. Only SNAT2 protein expression was increased but LAT1 showed relocalization from a perinuclear location to the cytoplasm and cell membrane. For in vivo studies, timed-pregnant animals were divided into four groups on day 18; sham-operated controls, uterine artery branch ligation (UABL), UABL + Ad-hIGF-1 (108 PFU), UABL + Ad-LacZ (108 PFU). At gestational day 20, pups and placentas were harvested by C-section. Only LAT1 mRNA expression changed, showing that a reduced expression of the transporter levels in the PI model could be partially rectified with Ad-hIGF1 treatment. At the protein level, System L was reduced in PI but remained at control levels following Ad-hIGF1. The System A isoforms were differentially regulated with SNAT2 expression diminished but SNAT1 increased in PI and Ad-hIGF1 groups. Enhanced

  5. Penetration dynamics of AP8 in thin ceramic tiles

    NARCIS (Netherlands)

    Abadjieva, E.; Khoe, Y.S.

    2013-01-01

    The interaction of thin ceramic tiles with AP8 (WC core, 7,62 mm) at 1000 m/s velocity has been studied experimentally and numerically. “Thin” ceramic tiles refers here to ratio of the tile thickness (t) to the projectile diameter, (d), t/d@ 1, as they are both in the same order. The method applied

  6. Time Trials--An AP Physics Challenge Lab

    Science.gov (United States)

    Jones, David

    2009-01-01

    I have come to the conclusion that for high school physics classroom and laboratory experiences, simpler is better! In this paper I describe a very simple and effective lab experience that my AP students have thoroughly enjoyed year after year. I call this lab exercise "Time Trials." The experiment is simple in design and it is a lot of fun for…

  7. The AP Lever for Boosting Access, Success, and Equity

    Science.gov (United States)

    Roegman, Rachel; Hatch, Thomas

    2016-01-01

    Four New Jersey school districts worked together to increase student achievement by applying a number of strategies focused on getting traditionally underrepresented students to take more AP courses. The districts are members of the New Jersey Network of Superintendents (NJNS), comprising 15 superintendents who work together to develop systemwide…

  8. The New AP Chemistry Exam: Its Rationale, Content, and Scoring

    Science.gov (United States)

    Price, Paul D.; Kugel, Roger W.

    2014-01-01

    The 2013-2014 academic year marks the rollout of the redesigned advanced placement (AP) chemistry course and exam. There have been many questions as to why the course was redesigned and how the new examination will differ from its legacy version. In this article we give a brief overview of the legacy course and examine why a redesign occurred in…

  9. Integrating Particulate Representations into AP Chemistry and Introductory Chemistry Courses

    Science.gov (United States)

    Prilliman, Stephen G.

    2014-01-01

    The College Board's recently revised curriculum for advanced placement (AP) chemistry places a strong emphasis on conceptual understanding, including representations of particle phenomena. This change in emphasis is informed by years of research showing that students could perform algorithmic calculations but not explain those calculations…

  10. Stokes IQUV magnetic Doppler imaging of Ap stars - I. ESPaDOnS and NARVAL observations

    Science.gov (United States)

    Silvester, J.; Wade, G. A.; Kochukhov, O.; Bagnulo, S.; Folsom, C. P.; Hanes, D.

    2012-10-01

    In this paper we describe and evaluate new spectral line polarization observations obtained with the goal of mapping the surfaces of magnetic Ap stars in great detail. One hundred complete or partial Stokes IQUV sequences, corresponding to 297 individual polarized spectra, have been obtained for seven bright Ap stars using the Échelle SpectroPolarimetric Device for the Observation of Stars (ESPaDOnS) and NARVAL high-resolution spectropolarimeters. The targets span a range of masses from approximately 1.8 to 3.4 M⊙, a range of rotation periods from 2.56 to 6.80 d and a range of maximum longitudinal magnetic field strengths from 0.3 to over 4 kG. For three of the seven stars, we have obtained dense phase coverage sampling the entire rotational cycle. These data sets are suitable for immediate magnetic and chemical abundance surface mapping using magnetic Doppler imaging. For the remaining four stars, partial phase coverage has been obtained, and additional observations will be required in order to map the surfaces of these stars. The median signal-to-noise ratio of the reduced observations is over 700 per 1.8 km s-1 pixel. Spectra of all stars show Stokes V Zeeman signatures in essentially all individual lines, and most stars show clear Stokes QU signatures in many individual spectral lines. The observations provide a vastly improved data set compared to previous generations of observations in terms of signal-to-noise ratio, resolving power and measurement uncertainties. Measurement of the longitudinal magnetic field demonstrates that the data are internally consistent within computed uncertainties typically at the 50-100σ level. Data are also shown to be in excellent agreement with published observations and in qualitative agreement with the predictions of published surface structure models. In addition to providing the foundation for the next generation of surface maps of Ap stars, this study establishes the performance and stability of the ESPaDOnS and NARVAL

  11. Chemical stratification in the atmosphere of Ap star HD 133792. Regularized solution of the vertical inversion problem

    CERN Document Server

    Kochukhov, O; Ryabchikova, T A; Makaganyk, V; Bagnulo, S

    2006-01-01

    High spectral resolution studies of cool Ap stars reveal conspicuous anomalies of the shape and strength of many absorption lines. This is a signature of large atmospheric chemical gradients produced by the selective radiative levitation and gravitational settling of chemical species. Here we present a new approach to mapping the vertical chemical structures in stellar atmospheres. We have developed a regularized chemical inversion procedure that uses all information available in high-resolution stellar spectra. The new technique for the first time allowed us to recover chemical profiles without making a priori assumptions about the shape of chemical distributions. We have derived average abundances and applied the vertical inversion procedure to the high-resolution VLT UVES spectra of the weakly magnetic, cool Ap star HD 133792. Our analysis yielded improved estimates of the atmospheric parameters of HD 133792. We show that this star has negligible vsini and the mean magnetic field modulus =1.1+/-0.1 kG. We ...

  12. High Line

    OpenAIRE

    Jagielska, Anna

    2015-01-01

    This project examines The High Line in New York City and what is due to its success. The park, which runs through the meatpacking district in Manhattan, is on an old rail line that was abandoned from the 1980s. Two private locals, Joshua and Hammond, founded the non-profit organization “Friends of The High Line” and established the rail line into a public park. The aesthetics of the park is the wildness of the nature and the modern architecture in the middle of Manhattan mixed with a sustaina...

  13. Heparin (GAG-hed inhibits LCR activity of Human Papillomavirus type 18 by decreasing AP1 binding

    Directory of Open Access Journals (Sweden)

    López-Bayghen Esther

    2006-08-01

    Full Text Available Abstract Background High risk HPVs are causative agents of anogenital cancers. Viral E6 and E7 genes are continuously expressed and are largely responsible for the oncogenic activity of these viruses. Transcription of the E6 and E7 genes is controlled by the viral Long Control Region (LCR, plus several cellular transcription factors including AP1 and the viral protein E2. Within the LCR, the binding and activity of the transcription factor AP1 represents a key regulatory event in maintaining E6/E7 gene expression and uncontrolled cell proliferation. Glycosaminoglycans (GAGs, such as heparin, can inhibit tumour growth; they have also shown antiviral effects and inhibition of AP1 transcriptional activity. The purpose of this study was to test the heparinoid GAG-hed, as a possible antiviral and antitumoral agent in an HPV18 positive HeLa cell line. Methods Using in vivo and in vitro approaches we tested GAG-hed effects on HeLa tumour cell growth, cell proliferation and on the expression of HPV18 E6/E7 oncogenes. GAG-hed effects on AP1 binding to HPV18-LCR-DNA were tested by EMSA. Results We were able to record the antitumoral effect of GAG-hed in vivo by using as a model tumours induced by injection of HeLa cells into athymic female mice. The antiviral effect of GAG-hed resulted in the inhibition of LCR activity and, consequently, the inhibition of E6 and E7 transcription. A specific diminishing of cell proliferation rates was observed in HeLa but not in HPV-free colorectal adenocarcinoma cells. Treated HeLa cells did not undergo apoptosis but the percentage of cells in G2/M phase of the cell cycle was increased. We also detected that GAG-hed prevents the binding of the transcription factor AP1 to the LCR. Conclusion Direct interaction of GAG-hed with the components of the AP1 complex and subsequent interference with its ability to correctly bind specific sites within the viral LCR may contribute to the inhibition of E6/E7 transcription and cell

  14. Functional analysis of the two Brassica AP3 genes involved in apetalous and stamen carpelloid phenotypes.

    Directory of Open Access Journals (Sweden)

    Yanfeng Zhang

    Full Text Available The Arabidopsis homeotic genes APETALA3 (AP3 and PISTILLATA (PI are B genes which encode MADS-box transcription factors and specify petal and stamen identities. In the current study, the stamen carpelloid (SC mutants, HGMS and AMS, of B. rapa and B. napus were investigated and two types of AP3 genes, B.AP3.a and B.AP3.b, were functional characterized. B.AP3.a and B.AP3.b share high similarity in amino acid sequences except for 8 residues difference located at the C-terminus. Loss of this 8 residues in B.AP3.b led to the change of PI-derived motifs. Meanwhile, B.AP3.a specified petal and stamen development, whereas B.AP3.b only specified stamen development. In B. rapa, the mutations of both genes generated the SC mutant HGMS. In B. napus that contained two B.AP3.a and two B.AP3.b, loss of the two B.AP3.a functions was the key reason for the apetalous mutation, however, the loss-of-function in all four AP3 was related to the SC mutant AMS. We inferred that the 8 residues or the PI-derived motif in AP3 gene probably relates to petal formation.

  15. Stokes IQUV Magnetic Doppler Imaging of Ap stars I. ESPaDOnS and NARVAL Observations

    CERN Document Server

    Silvester, J; Kochukhov, O; Bagnulo, S; Folsom, C P; Hanes, D

    2012-01-01

    In this paper we describe and evaluate new spectral line polarisation observations obtained with the goal of mapping the surfaces of magnetic Ap stars in great detail. One hundred complete or partial Stokes IQUV sequences, corresponding to 297 individual polarised spectra, have been obtained for 7 bright Ap stars using the ESPaDOnS and NARVAL spectropolarimeters. The targets span a range of mass from approximately 1.8 to 3.4 solar mass, a range of rotation period from 2.56 to 6.80 days, and a range of maximum longitudinal magnetic field strength from 0.3 to over 4 kG. For 3 of the 7 stars, we have obtained dense phase coverage sampling the entire rotational cycle. These datasets are suitable for immediate magnetic and chemical abundance surface mapping using Magnetic Doppler Imaging (MDI). For the 4 remaining stars, partial phase coverage has been obtained, and additional observations will be required in order to map the surfaces of these stars. The median signal-to-noise ratio of the reduced observations is ...

  16. KIC 7582608: a new Kepler roAp star with frequency variability

    Directory of Open Access Journals (Sweden)

    Holdsworth D.L.

    2015-01-01

    Full Text Available We analyse the fifth roAp star reported in the Kepler field, KIC 7582608, discovered with the SuperWASP project. The object shows a high frequency pulsation at 181.7324d−1 (P = 7.9 min with an amplitude of 1.45 mmag, and low frequency rotational modulation corresponding to a period of 20.4339 d. Spectral analysis confirms the Ap nature of the target, with characteristic lines of rare earth elements present. From our spectral observations we derive a lower limit on the mean magnetic field modulus of 〈B〉 =3.05 ± 0.23 kG. Long Cadence Kepler observations show a frequency quintuplet split by the rotational period of the star, typical for an oblique pulsator. We suggest the star is a quadrupole pulsator with a geometry such that i ~ 66° and β ~ 33°. We detect frequency variations of the pulsation in both the WASP and Kepler data sets on many time scales. Linear, non-adiabatic stability modelling allows us to constrain a region on the HR diagram where the pulsations are unstable, an area consistent with observations.

  17. Blurring lines

    OpenAIRE

    Howard, Heidi C; Knoppers, Bartha Maria; Borry, Pascal

    2010-01-01

    Companies that offer direct-to-consumer genetic testing and conduct research are blurring the line between customers and research subjects. Should these companies adhere to the same ethical regulations as academic researchers?

  18. High Line

    DEFF Research Database (Denmark)

    Kiib, Hans

    2015-01-01

    At just over 10 meters above street level, the High Line extends three kilometers through three districts of Southwestern Manhattan in New York. It consists of simple steel construction, and previously served as an elevated rail line connection between Penn Station on 34th Street and the many....... The High Line project has been carried out as part of an open conversion strategy. The result is a remarkable urban architectural project, which works as a catalyst for the urban development of Western Manhattan. The greater project includes the restoration and reuse of many old industrial buildings...... in close proximity to the park bridge and new projects being added to fit the context. The outcome is a conglomeration of non-contemporary urban activities along the High Line, where mechanical workshops, small wholesale stores. etc. mix with new exclusive residential buildings, eminent cafés...

  19. Functional Analysis of the Two Brassica AP3 Genes Involved in Apetalous and Stamen Carpelloid Phenotypes

    OpenAIRE

    Zhang, Yanfeng; Wang, Xuefang; Zhang, Wenxue; Yu, Fei; Tian, Jianhua; Li, Dianrong; Guo, Aiguang

    2011-01-01

    The Arabidopsis homeotic genes APETALA3 (AP3) and PISTILLATA (PI) are B genes which encode MADS-box transcription factors and specify petal and stamen identities. In the current study, the stamen carpelloid (SC) mutants, HGMS and AMS, of B. rapa and B. napus were investigated and two types of AP3 genes, B.AP3.a and B.AP3.b, were functional characterized. B.AP3.a and B.AP3.b share high similarity in amino acid sequences except for 8 residues difference located at the C-terminus. Loss of this 8...

  20. AP24534, a Pan-BCR-ABL Inhibitor for Chronic Myeloid Leukemia, Potently Inhibits the T315I Mutant and Overcomes Mutation-Based Resistance

    Energy Technology Data Exchange (ETDEWEB)

    O’Hare, Thomas; Shakespeare, William C.; Zhu, Xiaotian; Eide, Christopher A.; Rivera, Victor M.; Wang, Frank; Adrian, Lauren T.; Zhou, Tianjun; Huang, Wei-Sheng; Xu, Qihong; Metcalf, III, Chester A.; Tyner, Jeffrey W.; Loriaux, Marc M.; Corbin, Amie S.; Wardwell, Scott; Ning, Yaoyu; Keats, Jeffrey A.; Wang, Yihan; Sundaramoorthi, Raji; Thomas, Mathew; Zhou, Dong; Snodgrass, Joseph; Commodore, Lois; Sawyer, Tomi K.; Dalgarno, David C.; Deininger, Michael W.N.; Druker, Brian J.; Clackson, Tim; (OHSU- Cancer Instit.); (ARIAD)

    2010-09-07

    Inhibition of BCR-ABL by imatinib induces durable responses in many patients with chronic myeloid leukemia (CML), but resistance attributable to kinase domain mutations can lead to relapse and a switch to second-line therapy with nilotinib or dasatinib. Despite three approved therapeutic options, the cross-resistant BCR-ABL{sup T315I} mutation and compound mutants selected on sequential inhibitor therapy remain major clinical challenges. We report design and preclinical evaluation of AP24534, a potent, orally available multitargeted kinase inhibitor active against T315I and other BCR-ABL mutants. AP24534 inhibited all tested BCR-ABL mutants in cellular and biochemical assays, suppressed BCR-ABL{sup T315I}-driven tumor growth in mice, and completely abrogated resistance in cell-based mutagenesis screens. Our work supports clinical evaluation of AP24534 as a pan-BCR-ABL inhibitor for treatment of CML.

  1. DNA Charge Transport: Conformationally Gated Hopping through Stacked Domains

    OpenAIRE

    O'Neill, Melanie A.; Barton, Jacqueline K.

    2004-01-01

    The role of base motions in delocalization and propagation of charge through double helical DNA must be established experimentally and incorporated into mechanistic descriptions of DNA-mediated charge transport (CT). Here, we address these fundamental issues by examining the temperature dependence of the yield of CT between photoexcited 2-aminopurine (Ap*) and G through DNA bridges of varied length and sequence. DNA assemblies (35-mers) were constructed containing adenine bridges Ap(A)_nG (n ...

  2. On-line atomic data access

    International Nuclear Information System (INIS)

    The need for atomic data is one which continues to expand in a wide variety of applications including fusion energy, astrophysics, laser-produced plasma research, and plasma processing. Modern computer database and communications technology enables this data to be placed on-line and obtained by users over the INTERNET. Presented here is a summary of the observations and conclusions regarding such on-line atomic data access derived from a forum held at the Tenth APS Topical Conference on Atomic Processes in Plasmas. copyright 1996 American Institute of Physics

  3. Status of APS 1-Mwe Parabolic Trough Project

    Energy Technology Data Exchange (ETDEWEB)

    Canada, S.; Brosseau, D.; Kolb, G.; Moore, L.; Cable, R.; Price, H.

    2005-11-01

    Arizona Public Service (APS) is currently installing new power facilities to generate a portion of its electricity from solar resources that will satisfy its obligation under the Arizona Environmental Portfolio Standard (EPS). During FY04, APS began construction on a 1-MWe parabolic trough concentrating solar power plant. This plant represents the first parabolic trough plant to begin construction since 1991. Site preparation and construction activities continued throughout much of FY05, and startup activities are planned for Fall 2005 (with completion early in FY06). The plant will be the first commercial deployment of the Solargenix parabolic trough collector technology developed under contract to the National Renewable Energy Laboratory. The plant will use an organic Rankine cycle (ORC) power plant, provided by Ormat. The ORC power plant is much simpler than the conventional steam Rankine cycle plant and allows unattended operation of the facility.

  4. Comparative advantages of AP1000 passive safety system

    International Nuclear Information System (INIS)

    With the increasing need of nuclear power, almost all countries who want to build new nuclear power plant take the generation Ⅲ technology as their first choice. The main reason is that the safety of generation Ⅲ technology has been improved greatly than generation Ⅱ and Ⅱ + technology. The passive safety technology is introduced by AP1000 and it is one of the best in generation Ⅲ technologies. The representative passive containment cooling system of AP1000 and containment spray system of generation Ⅱ nuclear power plant were compared in the paper and analyzed using probabilistic safety assessment method. The reasons of passive safety system possessing comparative advantages were obtained by the concrete calculation. (authors)

  5. The magnetic fields of Ap stars from high resolution Stokes IQUV spectropolarimetry

    Science.gov (United States)

    Silvester, James

    In this thesis we describe the acquisition of high resolution time resolved spectropolarimetric observations of 7 (bright and well understood) Ap stars in Stokes IQUV using the ESPaDOnS and Narval spectropolarimeters at the Canada-France-Hawaii Telescope and the 2m Telescope Bernard Lyot at Pic du Midi Observatory. We compare these observations with those obtained a decade earlier using the MuSiCoS spectropolarimeter to confirm consistency with the older data and provide evidence that both ESPaDOnS and Narval perform as expected in all Stokes parameters. We demonstrate that our refined longitudinal magnetic field and linear polarisation measurements for these 7 stars are of much greater quality than was previously obtained with MuSiCoS and that the global magnetic properties of these stars are stable over a long timescale. The ultimate aim of these new data is to provide a basis from which mapping of both the magnetic field and abundance structures can be performed on our target stars. We then describe magnetic field mapping of the Ap star alpha 2 CVn using these data. This mapping is achieved with the use of tomographic inversion of Doppler-broadened Stokes IQUV profiles of a large variety of spectral lines using the INVERS10 Magnetic Doppler imaging code. We show that not only are the new magnetic field maps of alpha 2 CVn consistent with a previous generation of maps of alpha 2 CVn, but that the same magnetic field topology can be derived from a variety of atomic line sets. This indicates that the magnetic field we derive for alpha2 CVn is a realistic representation of the star's true magnetic topology. Finally we investigate surface abundance structures for alpha 2 CVn for various chemical elements. We investigate the correlation between the location of these abundance features and the magnetic field of alpha 2 CVn. We will demonstrate that whilst the magnetic field plays a role in the formation of abundance structures, the current theoretical framework does

  6. Diffusion and Settling in Ap/Bp Stars

    Energy Technology Data Exchange (ETDEWEB)

    Turcotte, S

    2003-04-09

    Ap/Bp stars are magnetic chemically peculiar early A and late B type stars of the main sequence. They exhibit peculiar surface abundance anomalies that are thought to be the result of gravitational settling and radiative levitation. The physics of diffusion in these stars are reviewed briefly and some model predictions are discussed. While models reproduce some observations reasonably well, more work is needed before the behavior of diffusing elements in a complex magnetic field is fully understood.

  7. Diffusion and Settling in Ap/Bp Stars

    CERN Document Server

    Turcotte, S

    2003-01-01

    Ap/Bp stars are magnetic chemically peculiar early A and late B type stars of the main sequence. They exhibit peculiar surface abundance anomalies that are thought to be the result of gravitational settling and radiative levitation. The physics of diffusion in these stars are reviewed briefly and some model predictions are discussed. While models reproduce some observations reasonably well, more work is needed before the behavior of diffusing elements in a complex magnetic field is fully understood.

  8. Electron-cloud measurements and simulations for the APS

    OpenAIRE

    Furman, M.A.; Pivi, M.; Harkay, K.C.; Rosenberg, R. A.

    2001-01-01

    We compare experimental results with simulations of the electron cloud effect induced by a positron beam at the APS synchrotron light source at ANL, where the electron cloud effect has been observed and measured with dedicated probes. We find good agreement between simulations and measurements for reasonable values of certain secondary electron yield (SEY) parameters, most of which were extracted from recent bench measurements at SLAC.

  9. On the progenitor of the Type Ic supernova 2002ap

    NARCIS (Netherlands)

    Smartt, SJ; Ramirez-Ruiz, E; Gilmore, GF; Meikle, WPS; Ferguson, AMN; Knapen, JH

    2002-01-01

    This Letter presents wide-field optical and near-IR (UBVRIHalphaK') images of the galaxy M74 that were taken between 0.6 and 8.3 yr before the discovery of the Type Ic supernova 2002ap. We have located the position of the supernova on these images with an accuracy of 0."3. We find no sign of a proge

  10. Thermal analysis of the beam missteering in APS storage ring

    International Nuclear Information System (INIS)

    Several bending magnet beam missteering cases have been investigated for the 7-GeV storage ring of the Advanced Photon Source (APS). One of the critical missteering events is presented in this paper. Finite element analyses are performed to solve for both temperature and stress fields. Thermally induced deflections are determined by using beam bending theory. A safe current limit is established for the storage ring chambers

  11. Electron-cloud measurements and simulations for the APS

    International Nuclear Information System (INIS)

    We compare experimental results with simulations of the electron cloud effect induced by a positron beam at the APS synchrotron light source at ANL, where the electron cloud effect has been observed and measured with dedicated probes. We find good agreement between simulations and measurements for reasonable values of certain secondary electron yield (SEY) parameters, most of which were extracted from recent bench measurements at SLAC

  12. Data of evolutionary structure change: 1AP2D-1QLRD [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1AP2D-1QLRD 1AP2 1QLR D D EVQLQQSGAELVRPGASVKLSCTASGFNIKDDFMHWVKQ...RPEQGLEWIGRIDPANDNTKYAPKFQDKATIIADTSSNTAYLQLSSLTSEDTAVYYCARREVYSYYSPLDVWGAGTTVTVPSGS------------------------...--------------------------------------------------------------- EVQLQQWGAGLLKPSETLSLTCAVYGGSFSD...EEE EEEEE 0 1QLR D 1QLRD GEINH-SGST

  13. Work plan, AP-102 mixer pump removal and pump replacement

    International Nuclear Information System (INIS)

    The objective of this work plan is to plan the steps and estimate the costs required to remove the failed AP-102 mixer pump, and to plan and estimate the cost of the necessary design and specification work required to order a new, but modified, mixer pump including the pump and pump pit energy absorbing design. The main hardware required for the removal of the mixer is as follows: a flexible receiver and blast shield; a metal container for the pulled mixer pump; and a trailer and strongback to haul and manipulate the container. Additionally: a gamma scanning device will be needed to detect the radioactivity emanating from the mixer as it is pulled from the tank; a water spray system will be required to remove tank waste from the surface of the mixer as it is pulled from the AP-102 tank; and a lifting yoke to lift the mixer from the pump pit (the SY-101 Mixer Lifting Yoke will be used). A ''green house'' will have to be erected over the AP-102 pump pit and an experienced Hoisting and Rigging crew must be assembled and trained in mixer pump removal methods before the actual removal is undertaken

  14. Maternal AP determinants in the Drosophila oocyte and embryo.

    Science.gov (United States)

    Ma, Jun; He, Feng; Xie, Gengqiang; Deng, Wu-Min

    2016-09-01

    An animal embryo cannot initiate its journey of forming a new life on its own. It must rely on maternally provided resources and inputs to kick-start its developmental process. In Drosophila, the initial polarities of the embryo along both the anterior-posterior (AP) and dorsal-ventral (DV) axes are also specified by maternal determinants. Over the past several decades, genetic and molecular studies have identified and characterized such determinants, as well as the zygotic genetic regulatory networks that control patterning in the early embryo. Extensive studies of oogenesis have also led to a detailed knowledge of the cellular and molecular interactions that control the formation of a mature egg. Despite these efforts, oogenesis and embryogenesis have been studied largely as separate problems, except for qualitative aspects with regard to maternal regulation of the asymmetric localization of maternal determinants. Can oogenesis and embryogenesis be viewed from a unified perspective at a quantitative level, and can that improve our understanding of how robust embryonic patterning is achieved? Here, we discuss the basic knowledge of the regulatory mechanisms controlling oogenesis and embryonic patterning along the AP axis. We explore properties of the maternal Bicoid gradient in relation to embryo size in search for a unified framework for robust AP patterning. WIREs Dev Biol 2016, 5:562-581. doi: 10.1002/wdev.235 For further resources related to this article, please visit the WIREs website. PMID:27253156

  15. 49 CFR 236.723 - Circuit, double wire; line.

    Science.gov (United States)

    2010-10-01

    ... § 236.723 Circuit, double wire; line. An electric circuit not employing a common return wire; a circuit... 49 Transportation 4 2010-10-01 2010-10-01 false Circuit, double wire; line. 236.723 Section 236.723 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL...

  16. 49 CFR 192.719 - Transmission lines: Testing of repairs.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Transmission lines: Testing of repairs. 192.719... TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Maintenance § 192.719 Transmission lines: Testing of repairs. (a) Testing of replacement pipe. If a segment of transmission line...

  17. 49 CFR 192.745 - Valve maintenance: Transmission lines.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Valve maintenance: Transmission lines. 192.745... TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Maintenance § 192.745 Valve maintenance: Transmission lines. (a) Each transmission line valve that might be required during any...

  18. FRANX. Application for analysis and quantification of the APS fire; FRANK. Aplicacion para el analisis y cuantificacion de los APS de incendios

    Energy Technology Data Exchange (ETDEWEB)

    Snchez, A.; Osorio, F.; Ontoso, N.

    2014-07-01

    The FRANX application has been developed by EPRI within the Risk and Reliability User Group in order to facilitate the process of quantification and updating APS Fire (also covers floods and earthquakes). By applying fire scenarios are quantified in the central integrating the tasks performed during the APS fire. This paper describes the main features of the program to allow quantification of an APS Fire. (Author)

  19. Development and validation of the Affect in Play Scale-brief rating version (APS-BR).

    Science.gov (United States)

    Cordiano, Tori J Sacha; Russ, Sandra W; Short, Elizabeth J

    2008-01-01

    The Affect in Play Scale (APS; Russ, 1987, 2004) is one of few reliable, standardized measures of pretend play, yet the fact that it requires videotaping and extensive training to score compromises its clinical utility. In this study, we developed and validated a brief rating version (APS-BR) that does not require videotaping. Construct validity was established by comparing scores from the original APS and the APS-BR using an existing data set of videotaped play (n = 46). We examined associations between scores on the APS-BR and theoretically relevant measures of divergent thinking and emotional memories. Scores on the APS-BR related strongly to those on the APS, and the pattern of correlations for each scale and relevant criterion measures was similar in strength and direction, supporting the APS-BR as an alternate form of the APS. In addition, we completed a pilot study to examine the efficacy of using the APS-BR in its intended in vivo format (n = 28). Results from both studies suggest that the APS-BR is a promising brief measure of children's pretend play that can be substituted for the APS in clinical and research settings. PMID:18444095

  20. NRC confirmatory AP600 safety system phase I testing in the ROSA/AP600 test facility

    Energy Technology Data Exchange (ETDEWEB)

    Rhee, G.S.; Kukita, Yutaka; Schultz, R.R.

    1996-03-01

    The NRC confirmatory phase I testing for the AP600 safety systems has been completed in the modified ROSA (Rig of Safety Assessment) test facility located at the Japan Atomic Energy Research Institute (JAERI) campus in Tokai, Japan. The test matrix included a variety of accident scenarios covering both design and beyond-design basis accidents. The test results indicate the AP600 safety systems as reflected in ROSA appear to perform as designed and there is no danger of core heatup for the accident scenarios investigated. In addition, no detrimental system interactions nor adverse effects of non-safety systems on the safety system functions were identified. However, three phenomena of interest have been identified for further examination to determine whether they are relevant to the AP600 plant. Those three phenomena are: (1) a potential for water hammer caused by rapid condensation which may occur following the actuation of the automatic depressurization system (ADS), (2) a large thermal gradient in the cold leg pipe where cooled water returns from the passive residual heat removal system and forms a thermally stratified layer, and (3) system-wide oscillations initiating following the ADS stage 4 actuation and persisting until the liquid in the pressurizer drains and steam generation in the core becomes insignificant.

  1. Role of the tryptophan residue in the vicinity of the catalytic center of exonuclease III family AP endonucleases: AP site recognition mechanism

    OpenAIRE

    Kaneda, Kohichi; Sekiguchi, Junichi; Shida, Toshio

    2006-01-01

    The mechanisms by which AP endonucleases recognize AP sites have not yet been determined. Based on our previous study with Escherichia coli exonuclease III (ExoIII), the ExoIII family AP endonucleases probably recognize the DNA-pocket formed at an AP site. The indole ring of a conserved tryptophan residue in the vicinity of the catalytic site presumably intercalates into this pocket. To test this hypothesis, we constructed a series of mutants of ExoIII and human APE1. Trp-212 of ExoIII and Tr...

  2. The AP-1 transcription factor homolog Pf-AP-1 activates transcription of multiple biomineral proteins and potentially participates in Pinctada fucata biomineralization

    OpenAIRE

    Xiangnan Zheng; Minzhang Cheng; Liang Xiang; Jian Liang; Liping Xie; Rongqing Zhang

    2015-01-01

    Activator protein-1 (AP-1) is an important bZIP transcription factor that regulates a series of physiological processes by specifically activating transcription of several genes, and one of its well-chartered functions in mammals is participating in bone mineralization. We isolated and cloned the complete cDNA of a Jun/AP-1 homolog from Pinctada fucata and called it Pf-AP-1. Pf-AP-1 had a highly conserved bZIP region and phosphorylation sites compared with those from mammals. A tissue distrib...

  3. The appearance of beam lines

    Energy Technology Data Exchange (ETDEWEB)

    Carey, D.C.

    1993-05-01

    The combination of an existing graphics package with a large program like TRANSPORT has often resulted in considerable modification to the large program. Use of other graphics package has resulted in essentially having to repeat the work. This difficulty has been avoided in a modification of TRANSPORT which produce layouts of beam lines. Drawings of the reference trajectory and three-dimensional images of all magnets are made by the graphics package TOP DRAWER. Nothing specific to TOP DRAWER or any other graphics has been incorporated into TRANSPORT. If a user is with a different graphics package he or she can then begin usage of this alternate package essentially immediately.

  4. Asymptotic-preserving methods for hyperbolic and transport equations with random inputs and diffusive scalings

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Shi, E-mail: jin@math.wisc.edu [Department of Mathematics, Institute of Natural Sciences and MOE-LSC, Shanghai Jiao Tong University, Shanghai 200240 (China); Department of Mathematics, University of Wisconsin, Madison, WI 53706 (United States); Xiu, Dongbin, E-mail: dongbin.xiu@utah.edu [Scientific Computing and Imaging Institute, University of Utah, Salt Lake City, UT 84112 (United States); Department of Mathematics, University of Utah, Salt Lake City, UT 84112 (United States); Zhu, Xueyu, E-mail: xzhu@sci.utah.edu [Scientific Computing and Imaging Institute, University of Utah, Salt Lake City, UT 84112 (United States)

    2015-05-15

    In this paper we develop a set of stochastic numerical schemes for hyperbolic and transport equations with diffusive scalings and subject to random inputs. The schemes are asymptotic preserving (AP), in the sense that they preserve the diffusive limits of the equations in discrete setting, without requiring excessive refinement of the discretization. Our stochastic AP schemes are extensions of the well-developed deterministic AP schemes. To handle the random inputs, we employ generalized polynomial chaos (gPC) expansion and combine it with stochastic Galerkin procedure. We apply the gPC Galerkin scheme to a set of representative hyperbolic and transport equations and establish the AP property in the stochastic setting. We then provide several numerical examples to illustrate the accuracy and effectiveness of the stochastic AP schemes.

  5. AP4 directly downregulates p16 and p21 to suppress senescence and mediate transformation

    OpenAIRE

    Jackstadt, R; Jung, P.; Hermeking, H

    2013-01-01

    Here we analyzed the function of the c-MYC-inducible basic helix–loop–helix leucine-zipper transcription factor AP4 in AP4-deficient mouse embryo fibroblasts (MEFs). Loss of AP4 resulted in premature senescence and resistance towards immortalization. Senescence was accompanied by induction of the cyclin-dependent kinase inhibitor-encoding genes p16, a known tumor suppressor, and p21, a previously described target for repression by AP4. Notably, AP4 directly repressed p16 expression via conser...

  6. Packaging design criteria, transfer and disposal of 102-AP mixer pump

    International Nuclear Information System (INIS)

    A mixer pump installed in storage tank 241-AP-102 (102-AP) has failed. This pump is referred to as the 102-AP mixer pump (APMP). The APMP will be removed from 102-AP 1 and a new pump will be installed. The main purpose of the Packaging Design Criteria (PDC) is to establish criteria necessary to design and fabricate a shipping container for the transfer and storage of the APMP from 102-AP. The PDC will be used as a guide to develop a Safety Evaluation for Packaging (SEP)

  7. NF-κB/AP-1-Targeted Inhibition of Macrophage-Mediated Inflammatory Responses by Depigmenting Compound AP736 Derived from Natural 1,3-Diphenylpropane Skeleton

    Directory of Open Access Journals (Sweden)

    Van Thai Ha

    2014-01-01

    Full Text Available AP736 was identified as an antimelanogenic drug that can be used for the prevention of melasma, freckles, and dark spots in skin by acting as a suppressor of melanin synthesis and tyrosinase expression. Since macrophage-mediated inflammatory responses are critical for skin health, here we investigated the potential anti-inflammatory activity of AP736. The effects of AP736 on various inflammatory events such as nitric oxide (NO/prostaglandin (PG E2 production, inflammatory gene expression, phagocytic uptake, and morphological changes were examined in RAW264.7 cells. AP736 was found to strongly inhibit the production of both NO and PGE2 in lipopolysaccharide- (LPS- treated RAW264.7 cells. In addition, AP736 strongly inhibited both LPS-induced morphological changes and FITC-dextran-induced phagocytic uptake. Furthermore, AP736 also downregulated the expression of multiple inflammatory genes, such as inducible NO synthase (iNOS, cyclooxygenase- (COX- 2, and interleukin- (IL- 1β in LPS-treated RAW264.7 cells. Transcription factor analysis, including upstream signalling events, revealed that both NF-κB and AP-1 were targeted by AP736 via inhibition of the IKK/IκBα and IRAK1/TAK1 pathways. Therefore, our results strongly suggest that AP736 is a potential anti-inflammatory drug due to its suppression of NF-κB-IKK/IκBα and AP-1-IRAK1/TAK1 signalling, which may make AP736 useful for the treatment of macrophage-mediated skin inflammation.

  8. Differentiation of zebrafish melanophores depends on transcription factors AP2 alpha and AP2 epsilon.

    Directory of Open Access Journals (Sweden)

    Eric Van Otterloo

    2010-09-01

    Full Text Available A model of the gene-regulatory-network (GRN, governing growth, survival, and differentiation of melanocytes, has emerged from studies of mouse coat color mutants and melanoma cell lines. In this model, Transcription Factor Activator Protein 2 alpha (TFAP2A contributes to melanocyte development by activating expression of the gene encoding the receptor tyrosine kinase Kit. Next, ligand-bound Kit stimulates a pathway activating transcription factor Microphthalmia (Mitf, which promotes differentiation and survival of melanocytes by activating expression of Tyrosinase family members, Bcl2, and other genes. The model predicts that in both Tfap2a and Kit null mutants there will be a phenotype of reduced melanocytes and that, because Tfap2a acts upstream of Kit, this phenotype will be more severe, or at least as severe as, in Tfap2a null mutants in comparison to Kit null mutants. Unexpectedly, this is not the case in zebrafish or mouse. Because many Tfap2 family members have identical DNA-binding specificity, we reasoned that another Tfap2 family member may work redundantly with Tfap2a in promoting Kit expression. We report that tfap2e is expressed in melanoblasts and melanophores in zebrafish embryos and that its orthologue, TFAP2E, is expressed in human melanocytes. We provide evidence that Tfap2e functions redundantly with Tfap2a to maintain kita expression in zebrafish embryonic melanophores. Further, we show that, in contrast to in kita mutants where embryonic melanophores appear to differentiate normally, in tfap2a/e doubly-deficient embryonic melanophores are small and under-melanized, although they retain expression of mitfa. Interestingly, forcing expression of mitfa in tfap2a/e doubly-deficient embryos partially restores melanophore differentiation. These findings reveal that Tfap2 activity, mediated redundantly by Tfap2a and Tfap2e, promotes melanophore differentiation in parallel with Mitf by an effector other than Kit. This work

  9. Westinghouse Passive Plants - AP600 and S PWR

    International Nuclear Information System (INIS)

    The original thought behind the AP600 passive design was that if the U. S. nuclear industry was to be revitalized, it would require a new, advanced technology with clearly proven benefits in safety. Response from the international arena indicates that, regardless of local domestic consideration, a revitalization of the U. S. industry is seen as very important, even essential, worldwide. And the potential for scale up of these passive safety features has been clearly established, allowing the benefits of the passive technology to be realized in countries that, for whatever reason, are interested in larger plant sizes only. Government projections indicate that U. S. energy demands in the 1990s will grow steadily, creating the need for approximately 117,000 to 322,000 MW of new generating capacity by the year 2010. Although this growth in electricity demand continues to be strong, orders for new nuclear power plants have not kept pace, in part due to licensing delays, prohibitive construction costs, and public uncertainty about safety. However, with the increased concerns about the environmental and economic security risks involved with an excessive dependence on fossil fuels, there is a growing realization that nuclear power must play a major role in our energy future. Looking to the future, Westinghouse is developing the AP600, a simplified two-loop PWR featuring passive safety systems. Drawing on the results of the AP600 development and testing programs, Westinghouse is also developing the larger S PWR, a passive, three-loop power plant with an output in the 900 to 1000 MW range

  10. Molecular Basis for Enhancement of the Meiotic DMCI Recombinase by RAD51AP1

    Energy Technology Data Exchange (ETDEWEB)

    Dray, Eloise; Dunlop, Myun Hwa; Kauppi, Liisa; San Filippo, Joseph San; Wiese, Claudia; Tsai, Miaw-Sheue; Begovic, Sead; Schild, David; Jasin, Maria; Keeney, Scott; Sung, Patrick

    2010-11-05

    Homologous recombination is needed for meiotic chromosome segregation, genome maintenance, and tumor suppression. RAD51AP1 (RAD51 Associated Protein 1) has been shown to interact with and enhance the recombinase activity of RAD51. Accordingly, genetic ablation of RAD51AP1 leads to enhanced sensitivity to and also chromosome aberrations upon DNA damage, demonstrating a role for RAD51AP1 in mitotic homologous recombination. Here we show physical association of RAD51AP1 with the meiosis-specific recombinase DMC1 and a stimulatory effect of RAD51AP1 on the DMC1-mediated D-loop reaction. Mechanistic studies have revealed that RAD51AP1 enhances the ability of the DMC1 presynaptic filament to capture the duplex DNA partner and to assemble the synaptic complex, in which the recombining DNA strands are homologously aligned. We also provide evidence that functional co-operation is dependent on complex formation between DMC1 and RAD51AP1, and that distinct epitopes in RAD51AP1 mediate interactions with RAD51 and DMC1. Finally, we show that RAD51AP1 is expressed in mouse testes, and that RAD51AP1 foci co-localize with a subset of DMC1 foci in spermatocytes. These results suggest that RAD51AP1 also serves an important role in meiotic homologous recombination.

  11. A Fe K Line in GRB 970508

    Science.gov (United States)

    Protassov, R.; van Dyk, D.; Connors, A.; Kashyap, V.; Siemiginowska, A.

    2000-12-01

    We examine the x-ray spectrum of the afterglow of GRB 970508, analyzed for Fe line emission by Piro et al (1999, ApJL, 514, L73). This is a difficult and extremely important measurement: the detection of x-ray afterglows from γ -ray bursts is at best a tricky business, relying on near-real satellite time response to unpredictable events; and a great deal of luck in catching a burst bright enough for a useful spectral analysis. Detecting a clear atomic (or cyclotron) line in the generally smooth and featureless afterglow (or burst) emission not only gives one of the few very specific keys to the physics local to the emission region, but also provides clues or confirmation of its distance (via redshift). Unfortunately, neither the likelihood ratio test or the related F-statistic commonly used to detect spectral lines adhere to their nominal Chi square and F-distributions. Thus we begin by calibrating the F-statistic used in Piro et al (1999, ApJL, 514, L73) via a simulation study. The simulation study relies on a completely specified source model, i.e. we do Monte Carlo simulations with all model parameters fixed (so--called ``parametric bootstrapping''). Second, we employ the method of posterior predictive p-values to calibrate a LRT statistic while accounting for the uncertainty in the parameters of the source model. Our analysis reveals evidence for the Fe K line.

  12. Multiplicity among chemically peculiar stars II. Cool magnetic Ap stars

    OpenAIRE

    Carrier, F.; North, P.; Udry, S.; Babel, J.

    2002-01-01

    We present new orbits for sixteen Ap spectroscopic binaries, four of which might in fact be Am stars, and give their orbital elements. Four of them are SB2 systems: HD 5550, HD 22128, HD 56495 and HD 98088. The twelve other stars are : HD 9996, HD 12288, HD 40711, HD 54908, HD 65339, HD 73709, HD 105680, HD 138426, HD 184471, HD 188854, HD 200405 and HD 216533. Rough estimates of the individual masses of the components of HD 65339 (53 Cam) are given, combining our radial velocities with the r...

  13. Santiago Apóstol en la intimidad del santiaguero

    OpenAIRE

    Portuondo Zúñiga, Olga

    2010-01-01

    La relación entre el apóstol Santiago (el Mayor) y Santiago de Cuba remonta hasta el ya casi milenio de su fundación; y en el día de hoy, la ciudad está orgullosa de su nombre y de su patronazgo. La tradición engarza las cuentas de una historia común, sin que implique una plena identificación espiritual inicial, ni la inexistencia de escollos a salvar para que la comunidad asumiera como honra y nobleza su cualidad santiaguera.

  14. Operation of the APS photoinjector drive laser system.

    Energy Technology Data Exchange (ETDEWEB)

    Li, Y.; Accelerator Systems Division (APS)

    2008-08-04

    The APS photoinjector drive laser system has been in operation since 1999 and is achieving a performance level exceeding the requirement of stable operation of the LEUTL FEL system. One remarkable number is the UV energy stability of better than 2% rms, sometimes less than 1% rms. This report summarizes the operation experience of the laser system and the improvements made along the way. We also outline the route of upgrade of the system and some frontier laser research and development opportunities in ultrabright electron beam generation.

  15. Euphorbesulins A-P, Structurally Diverse Diterpenoids from Euphorbia esula.

    Science.gov (United States)

    Zhou, Bin; Wu, Yan; Dalal, Seema; Cassera, Maria B; Yue, Jian-Min

    2016-08-26

    Aqueous ethanol extracts of powdered twigs of Euphorbia esula afforded 16 new diterpenoids, named euphorbesulins A-P. These euphorbesulins included presegetane (1-3), jatrophane (4-14), paraliane (15), and isopimarane (16) diterpenoids as well as six known analogues. Compounds 1-3 represent a rare type of presegetane diterpenoid. Their structures were determined by analysis of the spectroscopic data, and the absolute configuration of 1 was established by X-ray crystallography. Diterpenoid 7 showed low nanomolar antimalarial activity, while the remaining compounds showed only moderate or no antimalarial activity. PMID:27447736

  16. Transport policy

    OpenAIRE

    1980-01-01

    Transport is a fundamental component of all modern economies. Transport Policy presents a wide ranging collection of previously published articles which aim to provide the reader with an understanding of the main elements of transport policy.

  17. Expansion and Functional Divergence of AP2 Group Genes in Spermatophytes Determined by Molecular Evolution and Arabidopsis Mutant Analysis

    Science.gov (United States)

    Wang, Pengkai; Cheng, Tielong; Lu, Mengzhu; Liu, Guangxin; Li, Meiping; Shi, Jisen; Lu, Ye; Laux, Thomas; Chen, Jinhui

    2016-01-01

    The APETALA2 (AP2) genes represent the AP2 group within a large group of DNA-binding proteins called AP2/EREBP. The AP2 gene is functional and necessary for flower development, stem cell maintenance, and seed development, whereas the other members of AP2 group redundantly affect flowering time. Here we study the phylogeny of AP2 group genes in spermatophytes. Spermatophyte AP2 group genes can be classified into AP2 and TOE types, six clades, and we found that the AP2 group homologs in gymnosperms belong to the AP2 type, whereas TOE types are absent, which indicates the AP2 type gene are more ancient and TOE type was split out of AP2 type and losing the major function. In Brassicaceae, the expansion of AP2 and TOE type lead to the gene number of AP2 group were up to six. Purifying selection appears to have been the primary driving force of spermatophyte AP2 group evolution, although positive selection occurred in the AP2 clade. The transition from exon to intron of AtAP2 in Arabidopsis mutant leads to the loss of gene function and the same situation was found in AtTOE2. Combining this evolutionary analysis and published research, the results suggest that typical AP2 group genes may first appear in gymnosperms and diverged in angiosperms, following expansion of group members and functional differentiation. In angiosperms, AP2 genes (AP2 clade) inherited key functions from ancestors and other genes of AP2 group lost most function but just remained flowering time controlling in gene formation. In this study, the phylogenies of AP2 group genes in spermatophytes was analyzed, which supported the evidence for the research of gene functional evolution of AP2 group.

  18. Masas de agujeros negros en Narrow Line Seyfert 1

    Science.gov (United States)

    Schmidt, E.; Ferreiro, D.; Oio, G.; Vega, L.; Donoso, L.

    We describe two of the ways to estimate black hole masses in AGN, and then we estimate the black hole masses of 13 Narrow Line Seyfert 1 galaxies with the two methods: virial masses, using the correlation found by Greene & Ho (2005, ApJ, 630, 122); and the correlation found by Tremaine et al. (2002, ApJ, 574, 740). For this work we analyzed the optical spectroscopy data we obtained from CASLEO (San Juan). We compare the results obtained through both methods. FULL TEXT IN SPANISH

  19. KIC 4768731: a bright long-period roAp star in the Kepler Field

    CERN Document Server

    Smalley, B; Murphy, S J; Lehmann, H; Kurtz, D W; Holdsworth, D L; Cunha, M S; Balona, L A; Briquet, M; Bruntt, H; de Cat, P; Lampens, P; Thygesen, A O; Uytterhoeven, K

    2015-01-01

    We report the identification of 61.45 d^-1 (711.2 mu Hz) oscillations, with amplitudes of 62.6-mu mag, in KIC 4768731 (HD 225914) using Kepler photometry. This relatively bright (V=9.17) chemically peculiar star with spectral type A5 Vp SrCr(Eu) has previously been found to exhibit rotational modulation with a period of 5.21 d. Fourier analysis reveals a simple dipole pulsator with an amplitude that has remained stable over a 4-yr time span, but with a frequency that is variable. Analysis of high-resolution spectra yields stellar parameters of T_eff = 8100 +/- 200 K, log g = 4.0 +/- 0.2, [Fe/H] = +0.31 +/- 0.24 and v sin i = 14.8 +/- 1.6 km/s. Line profile variations caused by rotation are also evident. Lines of Sr, Cr, Eu, Mg and Si are strongest when the star is brightest, while Y and Ba vary in anti-phase with the other elements. The abundances of rare earth elements are only modestly enhanced compared to other roAp stars of similar T_eff and log g. Radial velocities in the literature suggest a significant...

  20. Radiation Transport

    Energy Technology Data Exchange (ETDEWEB)

    Urbatsch, Todd James [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-06-15

    We present an overview of radiation transport, covering terminology, blackbody raditation, opacities, Boltzmann transport theory, approximations to the transport equation. Next we introduce several transport methods. We present a section on Caseology, observing transport boundary layers. We briefly broach topics of software development, including verification and validation, and we close with a section on high energy-density experiments that highlight and support radiation transport.

  1. Line facilities outline

    International Nuclear Information System (INIS)

    This book deals with line facilities. The contents of this book are outline line of wire telecommunication ; development of line, classification of section of line and theory of transmission of line, cable line ; structure of line, line of cable in town, line out of town, domestic cable and other lines, Optical communication ; line of optical cable, transmission method, measurement of optical communication and cable of the sea bottom, Equipment of telecommunication line ; telecommunication line facilities and telecommunication of public works, construction of cable line and maintenance and Regulation of line equipment ; regulation on technique, construction and maintenance.

  2. production lines

    Directory of Open Access Journals (Sweden)

    Jingshan Li

    2000-01-01

    Full Text Available In this work, serial production lines with finished goods buffers operating in the pull regime are considered. The machines are assumed to obey Bernoulli reliability model. The problem of satisfying customers demand is addressed. The level of demand satisfaction is quantified by the due-time performance (DTP, which is defined as the probability to ship to the customer a required number of parts during a fixed time interval. Within this scenario, the definitions of DTP bottlenecks are introduced and a method for their identification is developed.

  3. DELTA : a computer code for determination of efficiency of particulate matter and aerosol transport; DELTA : un codice di calcolo per la determinazione dell`efficienza delle linee di prelievo e trasporto dell`aerosol

    Energy Technology Data Exchange (ETDEWEB)

    Picini, P.; Caropreso, G.; Antonini, A.; Galuppi, G.; Sbrana, M. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dip. Ambiente; Bardone, G. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dip. Energia; Malvestuto, V.; Ricotta, A. [CNR, Rome (Italy). Ist. di Fisica dell`Atmosfera

    1996-04-01

    In the Part I of this paper a mathematical model to calculate the sampling and transport efficiencies (both in laminar and turbulent condition) of any sampling and transport system decomposable in several cylindrical elemental component is presented. In the Part II an experimental facility built in Casaccia ENEA laboratory is described and the measures carried out to validate the model are reported.

  4. Loss of p12CDK2-AP1 Expression in Human Oral Squamous Cell Carcinoma with Disrupted Transforming Growth Factor-β-Smad Signaling Pathway

    Directory of Open Access Journals (Sweden)

    Hui Peng

    2006-12-01

    Full Text Available We examined correlations between TGF-β1, TβR-I and TβR-II, p12CDK2-AP1 p21WAF1 p27KIP1 Smad2, and p-Smad2 in 125 cases of human oral squamous cell carcinoma (OSCC to test the hypothesis that resistance to TGF-β1-induced growth suppression is due to the disruption of its signaling pathway as a consequence of reduced or lost p12CDK2-AP1. Immunoreactivity for TβR-II decreased in OSCC with increasing disease aggressiveness; however, no differences were observed for TβR-I and TGF-β1. The expression of TβR-II significantly correlated with p12CDK2-AP1 and p27KIP1 (P<.001 and P<.01, respectively. Furthermore, there was a significant relationship between TβR-II expression and p-Smad2 (P < .001. The in vivo correlation of the levels of TβR-II, p12CDK2-AP1 and p27 KIP1 was confirmed in normal and OSCC cell lines. Additionally, in vitro analysis of TGF-β-treated cells showed that TGF-β1 treatment of normal keratinocytes suppressed cell growth with upregulation of p-Smad2, p12CDK2-API and p21WAF1 expression, whereas there was no effect on OSCC cell lines. These results provide evidence of a link between a disrupted TGF-β-Smad signaling pathway and loss of induction of cell cycle-inhibitory proteins, especially p12CDK2-AP1 in OSCC, which may lead to the resistance of TGF-β1 growth-inhibitory effect on OSCC.

  5. Constitutive Expression of Sense & Antisense PtAP3, an AP3 Homologue Gene of Populus tomentosa, Affects Growth and Flowering Time in Transgenic Tobacco

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    To analyze the function of PtAP3, an APETALA3 (AP3) homologue gene isolated from Populus tomentosa Carr., the full length sequence (1 797 bp) and a fragment (870 bp) of PtAP3 were fused to a CaMV 35S promoter of pBI121 to generate the sense and antisense constructs of PtAP3. These constructs were transformed into tobacco by Agrobacterium infection of leaf disks and selection on kanamycin medium. Some sense and antisense transgenic tobacco plants were obtained by PCR and Southern blot analysis. Great phenotypic differences in transgenic tobacco plants were observed. Almost all of sense PtAP3 to transgenic tobaccos showed a higher growth rate than those of antisense transformants and a few developed pregnancy earlier than wild type seedlings and antisense transformants under the same conditions.

  6. The new AP Physics exams: Integrating qualitative and quantitative reasoning

    Science.gov (United States)

    Elby, Andrew

    2015-04-01

    When physics instructors and education researchers emphasize the importance of integrating qualitative and quantitative reasoning in problem solving, they usually mean using those types of reasoning serially and separately: first students should analyze the physical situation qualitatively/conceptually to figure out the relevant equations, then they should process those equations quantitatively to generate a solution, and finally they should use qualitative reasoning to check that answer for plausibility (Heller, Keith, & Anderson, 1992). The new AP Physics 1 and 2 exams will, of course, reward this approach to problem solving. But one kind of free response question will demand and reward a further integration of qualitative and quantitative reasoning, namely mathematical modeling and sense-making--inventing new equations to capture a physical situation and focusing on proportionalities, inverse proportionalities, and other functional relations to infer what the equation ``says'' about the physical world. In this talk, I discuss examples of these qualitative-quantitative translation questions, highlighting how they differ from both standard quantitative and standard qualitative questions. I then discuss the kinds of modeling activities that can help AP and college students develop these skills and habits of mind.

  7. Containment integrity analysis for the (W) advanced AP900

    International Nuclear Information System (INIS)

    The consequences of accidents that occurred at Three Mile Island and Chernobyl and the lack of interest from utilities in committing to high powered, high maintenance light water reactor designs of the past have motivated the nuclear industry to develop smaller, lower powered, simplified designs. Simplified designs must employ more passive systems that require very low maintenance and rely on the basic laws of nature. These designs must not be limited only to the reactor, but must also include the containment building. The purpose of this paper is to discuss the design concepts and to verify the feasibility of the Westinghouse Passive Containment Cooling System (PCCS) design for a 900 MWe advanced passive reactor design (AP900) by presenting its response to a design basis large break Loss of Coolant Accident (LOCA). The mass and energy releases emanating from the design basis break were developed and a representative containment model was constructed. The COMPACT computer code, which is a multi-node program that solves the complete set of mass, energy and momentum equations, was used to determine the AP900 containment response. The PCCS design concept, the computer model, the analysis inputs, and the results of the study are discussed within this paper. (author). 2 refs., 9 figs

  8. Preparation of and porosity measurements on oxidic APS spray coatings; Praeparation und Porositaetsmessung an oxidischen APS-Spritzschichten

    Energy Technology Data Exchange (ETDEWEB)

    Motl, Wolfgang [H.C. Starck GmbH, Laufenburg (Germany); Schnarr, Holger [Struers GmbH, Willich (Germany)

    2015-07-01

    Zirconium oxide coatings for application in the field of turbine construction have to exhibit certain properties in order to meet high temperature requirements. One of the most important properties is the porosity, for it provides a thermal insulation of the coated material. For this reason, the porosity is routinely determined as quality parameter for spray coatings which helps determining their suitability for a particular application. The following work summarizes the results of the round robin test ''Preparation of and Porosity Measurements on Oxidic APS Spray Coatings'' by the DGM working group ''Preparation''.

  9. Progresses on plant AP2/ERF transcription factors%植物AP2/ERF 类转录因子研究进展

    Institute of Scientific and Technical Information of China (English)

    张计育; 王庆菊; 郭忠仁

    2012-01-01

    植物AP2/ERF 是一个庞大的转录因子基因家族,含有由60~70 个氨基酸组成的AP2/ERF 结构域而得名,存在于所有的植物中.AP2/ERF 转录因子参与多种生物学过程,包括植物生长、花发育、果实发育、种子发育、损伤、病菌防御、高盐、干旱等环境胁迫响应等.AP2/ERF 类转录因子参与水杨酸、茉莉酸、乙烯、脱落酸等多种信号转导途径,而且是逆境信号交叉途径中的连接因子.文章对国内外近年来有关植物AP2/ERF 类转录因子的分类、生物学功能、基因调控等方面的研究进行了综述.%Plant AP2/ERF transcription factor with AP2/ERF domain containing 60-70 amino acids is a huge gene family present in all plant. AP2/ERF transcriptional factors are involved in various biological functions such as plant development, flower development, fruit and seed maturation, wounding, pathogen defense, high salty, drought, and so on. AP2/ERF transcription factor are involved in salicylic acid, jasmonic acid, ethylene, abscisic acid signal transduction pathways and among them. The transcription factors are cross-talk factor in stress signal pathway. This paper summarizes the most advanced researches on types, biological functions, and gene regulations of plant AP2/ERF transcription factors.

  10. RAD51AP2, a novel vertebrate- and meiotic-specific protein, sharesa conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51

    Energy Technology Data Exchange (ETDEWEB)

    Kovalenko, Oleg V.; Wiese, Claudia; Schild, David

    2006-07-25

    Many interacting proteins regulate and/or assist the activities of RAD51, a recombinase which plays a critical role in both DNA repair and meiotic recombination. Yeast two-hybrid screening of a human testis cDNA library revealed a new protein, RAD51AP2 (RAD51 Associated Protein 2), that interacts strongly with RAD51. A full-length cDNA clone predicts a novel vertebrate specific protein of 1159 residues, and the RAD51AP2 transcript was observed only in meiotic tissue (i.e. adult testis and fetal ovary), suggesting a meiotic-specific function for RAD51AP2. In HEK293 cells the interaction of RAD51 with an ectopically-expressed recombinant large fragment of RAD51AP2 requires the C-terminal 57 residues of RAD51AP2. This RAD51-binding region shows 81% homology to the C-terminus of RAD51AP1/PIR51, an otherwise totally unrelated RAD51-binding partner that is ubiquitously expressed. Analyses using truncations and point mutations in both RAD51AP1 and RAD51AP2 demonstrate that these proteins use the same structural motif for RAD51 binding. RAD54 shares some homology with this RAD51-binding motif, but this homologous region plays only an accessory role to the adjacent main RAD51-interacting region, which has been narrowed here to 40 amino acids. A novel protein, RAD51AP2, has been discovered that interacts with RAD51 through a C-terminal motif also present in RAD51AP1.

  11. Duplication of AP1 within the Spinacia oleracea L. AP1/FUL clade is followed by rapid amino acid and regulatory evolution.

    Science.gov (United States)

    Sather, D Noah; Golenberg, Edward M

    2009-02-01

    The AP1/FUL clade of MADS box genes have undergone multiple duplication events among angiosperm species. While initially identified as having floral meristem identity and floral organ identity function in Arabidopsis, the role of AP1 homologs does not appear to be universally conserved even among eudicots. In comparison, the role of FRUITFULL has not been extensively explored in non-model species. We report on the isolation of three AP1/FUL genes from cultivated spinach, Spinacia oleracea L. Two genes, designated SpAPETALA1-1 (SpAP1-1) and SpAPETALA1-2 (SpAP1-2), cluster as paralogous genes within the Caryophyllales AP1 clade. They are highly differentiated in the 3', carboxyl-end encoding region of the gene following the third amphipathic alpha-helix region, while still retaining some elements of a signature AP1 carboxyl motifs. In situ hybridization studies also demonstrate that the two paralogs have evolved different temporal and spatial expression patterns, and that neither gene is expressed in the developing sepal whorl, suggesting that the AP1 floral organ identity function is not conserved in spinach. The spinach FRUITFULL homolog, SpFRUITFULL (SpFUL), has retained the conserved motif and groups with Caryophyllales FRUITFULL homologs. SpFUL is expressed in leaf as well as in floral tissue, and shows strong expression late in flower development, particularly in the tapetal layer in males, and in the endothecium layer and stigma, in the females. The combined evidence of high rates of non-synonymous substitutions and differential expression patterns supports a scenario in which the AP1 homologs in the spinach AP1/FUL gene family have experienced rapid evolution following duplication.

  12. 49 CFR 192.706 - Transmission lines: Leakage surveys.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Transmission lines: Leakage surveys. 192.706... Transmission lines: Leakage surveys. Leakage surveys of a transmission line must be conducted at intervals not exceeding 15 months, but at least once each calendar year. However, in the case of a transmission line...

  13. 49 CFR 192.365 - Service lines: Location of valves.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Service lines: Location of valves. 192.365 Section..., and Service Lines § 192.365 Service lines: Location of valves. (a) Relation to regulator or meter. Each service-line valve must be installed upstream of the regulator or, if there is no...

  14. Induction of neural crest in Xenopus by transcription factor AP2alpha.

    Science.gov (United States)

    Luo, Ting; Lee, Young-Hoon; Saint-Jeannet, Jean-Pierre; Sargent, Thomas D

    2003-01-21

    We report experiments with Xenopus laevis, using both intact embryos and ectodermal explants, showing that the transcription factor AP2alpha is positively regulated by bone morphogenetic protein (BMP) and Wnt signaling, and that this activation is an essential step in the induction of neural crest (NC). Ectopic expression of AP2alpha is sufficient to activate high-level expression of NC-specific genes such as Slug and Sox9, which can occur as isolated domains within the neural plate as well as by expansion of endogenous NC territories. AP2alpha also has the property of inducing NC in isolated ectoderm in which Wnt signaling is provided but BMP signaling is minimized by overexpression of chordin. Like other NC regulatory factors, activation of AP2alpha requires some attenuation of endogenous BMP signaling; however, this process occurs at a lower threshold for AP2alpha. Furthermore, AP2alpha expression domains are larger than for other NC factors. Loss-of-function experiments with antisense AP2alpha morpholino oligonucleotides result in severe reduction in the NC territory. These results support a central role for AP2alpha in NC induction. We propose a model in which AP2alpha expression, along with inactivation of NC inhibitory factors such as Dlx3, establish a feedback loop comprising AP2alpha, Sox9, and Slug, leading to and maintaining NC specification. PMID:12511599

  15. Stokes IQUV magnetic Doppler imaging of Ap stars - II. Next generation magnetic Doppler imaging of α2 CVn

    Science.gov (United States)

    Silvester, J.; Kochukhov, O.; Wade, G. A.

    2014-05-01

    We present updated magnetic field maps of the chemically peculiar B9p star α2 CVn created using a series of time-resolved observations obtained using the high-resolution spectropolarimeters ESPaDOnS and Narval. We compare these new magnetic field maps with the original magnetic Doppler imaging maps based on spectra recorded with the MuSiCoS spectropolarimeter and taken a decade earlier. These new maps are inferred from line profiles in all four Stokes parameters using the magnetic Doppler imaging code INVERS10. With the addition of new lines exhibiting Stokes IQUV signatures, we have a unique insight into how the derived magnetic surface structure may be affected by the atomic lines chosen for inversion. We report new magnetic maps of α2 CVn created using strong iron lines (directly comparable to the published MuSiCoS maps), weak iron lines and chromium lines, all of which yield a magnetic field structure roughly consistent with that obtained previously. We then derive an updated magnetic structure map for α2 CVn based on the complete sample of Fe and Cr lines, which we believe to produce a more representative model of the magnetic topology of α2 CVn. In agreement with the previous mapping, this new updated magnetic map shows a dipolar-like field which has complex sub-structure which cannot be explained by a simple low-order multipolar geometry. Our new maps show that regardless of the atomic line or species choice, the reconstructed magnetic field is consistent with that published previously, suggesting that the reconstructed field is a realistic representation of the magnetic field of α2 CVn. α2 CVn is the first Ap star for which multiple, high-resolution magnetic maps have been derived, providing important observational evidence for the stability of both the large- and small-scale magnetic field.

  16. Characteristics of shigellosis outbreaks in the AP of Vojvodina

    Directory of Open Access Journals (Sweden)

    Đurić Predrag

    2009-01-01

    Full Text Available Introduction. Shigellosis causes around 165 million infections and around 1 million deaths in the world every year. Two thirds of both infections and deaths are among children younger than 10. Shigellosis mainly spreads by direct or indirect contact, but water- and food-borne outbreaks are not rare. Material and methods. A descriptive epidemiological method was used to analyse characteristics of shigellosis outbreaks in the AP of Vojvodina in the period 1979-2005. Results. During this period 9.083 shigellosis infections were registered, 51,9% of them in 202 shigellosis outbreaks. The leading way of transmission was contact, but water- and food-borne outbreaks were also detected. The average number of infections in contact outbreaks of shigellosis was small - 6.3 infections. The highest average is in water-borne outbreaks - 55.1. Most of the outbreaks were those occurring in families (47.5% and most of them were registered in august. Discussion. Shigellosis has been mandatory reported in the AP of Vojvodina since 1945 and results of outbreak investigation of this disease were analyzed since 1979. In the first half of this period water was the most common way of agent transmission in shigellosis outbreaks, but in recent 15 years the contact became the leading one. In the last 5 years there was no water-borne shigellosis outbreaks. Almost one third of all outbreaks were imported from Montenegro and Croatia by tourists coming back from those countries. Most of the outbreaks are small outbreaks occurring in families, primarily transmitted by contact. Outbreaks in facilities for care of disabled children and elders are also important. Sh. sonnei and Sh. flexneri are the two serotypes identified, the former in 57.4% and the latter in 40.1% outbreaks. Conclusion. Shigellosis is a disease occurring in the AP of Vojvodina primarily in small outbreaks inside families, transmitted by contact, most often in August and more often caused by Sh. sonnei. Health

  17. The flame structure of AP/HTPB sandwiches

    Science.gov (United States)

    Chorpening, Benjamin Todd

    2000-10-01

    Ultraviolet emission imaging experiments have been used to study the combustion of sandwiches of ammonium perchlorate (AP) and hydroxyl-terminated polybutadiene (HTPB) in nitrogen at pressures up to 32 atm, with binder layers from 50 to 450 mum in thickness. An ICCD camera system has been used to image the flame emission near 310 nm, and a backlighting technique has been developed that allows determination of the corresponding surface shape during combustion. The results indicate the AP/HTPB interface regression rate of IPDI cured samples undergoing low power (100W) laser-assisted deflagration is nearly independent of the binder thickness for binders thicker than 100 mum. The pressure exponent of the regression rate is 0.31 up to 15 atm, increasing with pressure from 15 to 32 atm. Two primary regimes of flame behavior have been identified: a split flame base regime which occurs with high Peclet and Damkohler numbers, and a merged flame base regime which occurs with low Peclet and Damkohler numbers. A secondary regime, exhibiting a "lifted" flame, occurs with low Damkohler numbers and high Peclet numbers. The ultraviolet flame emissions observed in the experiments show a correspondence with the fuel-rich region of the flame, as determined with a Schvab-Zeldovich model. This is reasonable since the primary sources of ultraviolet emission in the 305--315 nm region, electronically excited OH and the CO + O reaction, are dependent on fuel related species. The growth of the fuel-rich region with increasing Peclet number, predicted by the model, is qualitatively matched by the experimental results. The predicted shrinkage of the fuel-rich region when the binder layer is diluted with fine AP is also qualitatively matched by the experiments. Comparison of the experimental results with a single-reaction model with finite rate kinetics shows a weak qualitative agreement on the influence of Damkohler number. A large increase in Damkohler number (factor of 20) leads to a strong

  18. Low-energy beam line at KVI

    NARCIS (Netherlands)

    Toprek, D.; Formanoy, I.; Brandenburg, S.

    2006-01-01

    This paper describes the redesign of the low-energy beam line at KVI. Redesigned and properties of the optical elements of the transport beam line is done by using the code COSY INFINITY in the third-order of approximation. The effects of fringe fields of the optical elements are also taken into acc

  19. Traffic Analysis Zones - TRAFFIC_COUNTS_INDOTMODEL_IN: Traffic Counts on Roadways (from INDOT Statewide Travel Demand MODEL version 4, ISTDM4) in Indiana (Indiana Department of Transportation, Line Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — TRAFFIC_COUNTS_INDOTMODEL_IN is a line shapefile that shows traffic counts (factored to year 2000) for the roadways in the Indiana Statewide Travel Demand Model,...

  20. The interaction between the adaptor protein APS and Enigma is involved in actin organisation

    DEFF Research Database (Denmark)

    Barres, Romain; Gonzalez, Teresa; Le Marchand-Brustel, Yannick;

    2005-01-01

    and APS were partially co-localised with F-actin in small ruffling structures. Insulin increased the complex formation between APS and Enigma and their co-localisation in large F-actin containing ruffles. While in NIH-3T3 and HeLa cells the co-expression of both Enigma and APS did not modify the actin...... cytoskeleton organisation, expression of Enigma alone led to the formation of F-actin clusters. Similar alteration in actin cytoskeleton organisation was observed in cells expressing both Enigma and APS with a mutation in the NPTY motif. These results identify Enigma as a novel APS-binding protein and suggest...... that the APS/Enigma complex plays a critical role in actin cytoskeleton organisation....

  1. Counting statistics and loss corrections for the APS

    International Nuclear Information System (INIS)

    It has been suggested that for timing experiments, it might be advantageous to arrange the bunches in the storage ring in an asymmetrical mode. In this paper, we determine the counting losses from pulsed x-ray sources from basic probabilistic arguments and from Poisson statistics. In particular the impact on single photon counting losses of a variety of possible filling modes for the Advanced Photon Source (APS) is examined. For bunches of equal current, a loss of 10% occurs whenever the count rate exceeds 21% of the bunch repetition rate. This changes slightly when bunches containing unequal numbers of particles are considered. The results are applied to several common detector/electronics systems

  2. An ancillary pumping system for the APS vacuum system

    Energy Technology Data Exchange (ETDEWEB)

    Walters, D.; Noonan, J.; Gagliano, J. [and others

    1997-06-01

    An ancillary pumping system has been designed and installed in the Advanced Photon Source (APS) storage ring. This vacuum system has the ability to pump sectors of the storage ring down from room pressure to ultra-high vacuum (UHV). The ancillary system is a {open_quotes}dry{close_quotes} system that uses a combination of turbomolecular pumps and oil-free roughing pumps. The benefits of this system are the reduction of equipment needed for in-tunnel maintenance, essential for the operation of a UHV storage ring; rapid response to vacuum emergencies and a guard to accidental exposures to hydrocarbon contamination. The operational logic and the pump interlock and controls are described.

  3. Autonomous star tracker based on active pixel sensors (APS)

    Science.gov (United States)

    Schmidt, U.

    2004-06-01

    Star trackers are opto-electronic sensors used onboard of satellites for the autonomous inertial attitude determination. During the last years, star trackers became more and more important in the field of the attitude and orbit control system (AOCS) sensors. High performance star trackers are based up today on charge coupled device (CCD) optical camera heads. The Jena-Optronik GmbH is active in the field of opto-electronic sensors like star trackers since the early 80-ties. Today, with the product family ASTRO5, ASTRO10 and ASTRO15, all marked segments like earth observation, scientific applications and geo-telecom are supplied to European and Overseas customers. A new generation of star trackers can be designed based on the APS detector technical features. The measurement performance of the current CCD based star trackers can be maintained, the star tracker functionality, reliability and robustness can be increased while the unit costs are saved.

  4. Towards a theory of rapidly oscillating Ap stars

    CERN Document Server

    Gough, Douglas

    2011-01-01

    Peculiar A stars are so named because they exhibit abundance peculiarities in their atmospheres. It is believed that these arise as a result of differentiation of chemical species in large magnetic spots in which convective mixing is inhibited: there might be just two antipodal spots, whose axis is inclined to the axis of rotation. Many of the Ap stars that are rotating slowly also pulsate, with periods substantially shorter than the period of the fundamental radial mode. The pulsations appear to be nonradial, but axisymmetric, with their common axis usually aligned with the axis of the spots. In this lecture I shall first discuss the magnetic suppression of convection in the spots, and then I shall try to explain the pulsation phenomenon, reviewing some of the suggestions that have been made to explain the alignment and the excitation mechanism, and finally raising some issues that need to be addressed.

  5. Fabrication and Testing of Deflecting Cavities for APS

    Energy Technology Data Exchange (ETDEWEB)

    Mammosser, John; Wang, Haipeng; Rimmer, Robert; Jim, Henry; Katherine, Wilson; Dhakal, Pashupati; Ali, Nassiri; Jim, Kerby; Jeremiah, Holzbauer; Genfa, Wu; Joel, Fuerst; Yawei, Yang; Zenghai, Li

    2013-09-01

    Jefferson Lab (Newport News, Virginia) in collaboration with Argonne National Laboratory (Argonne, IL) has fabricated and tested four first article, 2.8 GHz, deflecting SRF cavities, for Argonne's Short-Pulse X-ray (SPX) project. These cavities are unique in many ways including the fabrication techniques in which the cavity cell and waveguides were fabricated. These cavity subcomponents were milled from bulk large grain niobium ingot material directly from 3D CAD files. No forming of sub components was used with the exception of the beam-pipes. The challenging cavity and helium vessel design and fabrication results from the stringent RF performance requirements required by the project and operation in the APS ring. Production challenges and fabrication techniques as well as testing results will be discussed in this paper.

  6. Progress on the development of APS beam position monitoring system

    International Nuclear Information System (INIS)

    This paper describes the development status of the beam position monitoring system for the Advanced Photon Source (APS), a third-generation light source now under construction at Argonne National Laboratory. The accelerator complex will consist of an electron linac, a positron linac, a positron accumulator ring (PAR), an injector synchrotron and a storage ring. For beam position measurement, striplines will be used on the linacs, while button-type pickups will be used on the injector synchrotron and the storage ring. A test stand with a prototype injector synchrotron beam position monitor (BPM) unit has been built, and we present the results of position calibration measurements using a wire. Comparison of the results with theoretical calculations will be presented. The current effort on similar storage ring BPM system measurements will also be discussed. 4 refs., 5 figs., 2 tabs

  7. A novel cell line derived from pleomorphic adenoma expresses MMP2, MMP9, TIMP1, TIMP2, and shows numeric chromosomal anomalies.

    Directory of Open Access Journals (Sweden)

    Aline Semblano Carreira Falcão

    Full Text Available Pleomorphic adenoma is the most common salivary gland neoplasm, and it can be locally invasive, despite its slow growth. This study aimed to establish a novel cell line (AP-1 derived from a human pleomorphic adenoma sample to better understand local invasiveness of this tumor. AP-1 cell line was characterized by cell growth analysis, expression of epithelial and myoepithelial markers by immunofluorescence, electron microscopy, 3D cell culture assays, cytogenetic features and transcriptomic study. Expression of matrix metalloproteinases (MMPs and their tissue inhibitors (TIMPs was also analyzed by immunofluorescence and zymography. Furthermore, epithelial and myoepithelial markers, MMPs and TIMPs were studied in the tumor that originated the cell line. AP-1 cells showed neoplastic epithelial and myoepithelial markers, such as cytokeratins, vimentin, S100 protein and smooth-muscle actin. These molecules were also found in vivo, in the tumor that originated the cell line. MMPs and TIMPs were observed in vivo and in AP-1 cells. Growth curve showed that AP-1 exhibited a doubling time of 3.342 days. AP-1 cells grown inside Matrigel recapitulated tumor architecture. Different numerical and structural chromosomal anomalies were visualized in cytogenetic analysis. Transcriptomic analysis addressed expression of 7 target genes (VIM, TIMP2, MMP2, MMP9, TIMP1, ACTA2 e PLAG1. Results were compared to transcriptomic profile of non-neoplastic salivary gland cells (HSG. Only MMP9 was not expressed in both libraries, and VIM was expressed solely in AP-1 library. The major difference regarding gene expression level between AP-1 and HSG samples occurred for MMP2. This gene was 184 times more expressed in AP-1 cells. Our findings suggest that AP-1 cell line could be a useful model for further studies on pleomorphic adenoma biology.

  8. A novel cell line derived from pleomorphic adenoma expresses MMP2, MMP9, TIMP1, TIMP2, and shows numeric chromosomal anomalies.

    Science.gov (United States)

    Falcão, Aline Semblano Carreira; Kataoka, Maria Sueli da Silva; Ribeiro, Nélson Antonio Bailão; Diniz, José Antonio Picanço; Alves, Sérgio Melo; Ribeiro, André L Ribeiro; de Siqueira, Adriane Sousa; da Silva, Artur Luiz; Ramos, Rommel Thiago Jucá; Freitas, Vanessa M; Jaeger, Ruy G; Pinheiro, João J V

    2014-01-01

    Pleomorphic adenoma is the most common salivary gland neoplasm, and it can be locally invasive, despite its slow growth. This study aimed to establish a novel cell line (AP-1) derived from a human pleomorphic adenoma sample to better understand local invasiveness of this tumor. AP-1 cell line was characterized by cell growth analysis, expression of epithelial and myoepithelial markers by immunofluorescence, electron microscopy, 3D cell culture assays, cytogenetic features and transcriptomic study. Expression of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) was also analyzed by immunofluorescence and zymography. Furthermore, epithelial and myoepithelial markers, MMPs and TIMPs were studied in the tumor that originated the cell line. AP-1 cells showed neoplastic epithelial and myoepithelial markers, such as cytokeratins, vimentin, S100 protein and smooth-muscle actin. These molecules were also found in vivo, in the tumor that originated the cell line. MMPs and TIMPs were observed in vivo and in AP-1 cells. Growth curve showed that AP-1 exhibited a doubling time of 3.342 days. AP-1 cells grown inside Matrigel recapitulated tumor architecture. Different numerical and structural chromosomal anomalies were visualized in cytogenetic analysis. Transcriptomic analysis addressed expression of 7 target genes (VIM, TIMP2, MMP2, MMP9, TIMP1, ACTA2 e PLAG1). Results were compared to transcriptomic profile of non-neoplastic salivary gland cells (HSG). Only MMP9 was not expressed in both libraries, and VIM was expressed solely in AP-1 library. The major difference regarding gene expression level between AP-1 and HSG samples occurred for MMP2. This gene was 184 times more expressed in AP-1 cells. Our findings suggest that AP-1 cell line could be a useful model for further studies on pleomorphic adenoma biology.

  9. Study on the thermal decomposition behavior of RDX/AP/HTPB propellant%RDX/AP/HTPB推进剂热分解特性研究

    Institute of Scientific and Technical Information of China (English)

    陈沛; 赵凤起; 阴翠梅

    2002-01-01

    利用高压差示扫描量热仪(PDSC)研究了RDX/AP/HTPB推进剂系列配方的热分解性能,发现配方组分的改变对RDX/AP/HTPB推进剂的热分解性能有影响,突出表现在RDX/AP/HTPB推进剂中RDX分解峰变宽,AP放热分解效应增强.推进剂中添加Al粉后,RDX的分解受到抑制,而AP的分解却得到增强.

  10. Quality Assurance Audit Practice in AP1000 Nuclear Project%AP1000核电工程的监查管理实践

    Institute of Scientific and Technical Information of China (English)

    张华

    2015-01-01

    文中主要介绍了AP1000核电工程三门、海阳依托项目建立质保体系所依据的标准、法规,和实施质保监查使用的主要方式方法.结合AP1000监查活动实施的现状,总结了AP1000核电工程的监查实践和经验,为其它核电工程的质量管理提供了建议和参考.

  11. HPV16 E6 and E6AP differentially cooperate to stimulate or augment Wnt signaling

    Energy Technology Data Exchange (ETDEWEB)

    Sominsky, Sophia, E-mail: sophia.tab@gmail.com [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Kuslansky, Yael, E-mail: ykuslansky@gmail.com [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Shapiro, Beny, E-mail: benyshap@gmail.com [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Jackman, Anna, E-mail: jackman@post.tau.ac.il [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Haupt, Ygal, E-mail: ygal.haupt@petermac.org [Research Division, The Peter MacCallum Cancer Centre, East Melbourne (Australia); Rosin-Arbesfeld, Rina, E-mail: arina@post.tau.ac.il [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Sherman, Levana, E-mail: lsherman@post.tau.ac.il [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel)

    2014-11-15

    The present study investigated the roles of E6 and E6AP in the Wnt pathway. We showed that E6 levels are markedly reduced in cells in which Wnt signaling is activated. Coexpression of wild-type or mutant E6AP (C820A) in Wnt-activated cells stabilized E6 and enhanced Wnt/β-catenin/TCF transcription. Expression of E6AP alone in nonstimulated cells elevated β-catenin level, promoted its nuclear accumulation, and activated β-catenin/TCF transcription. A knockdown of E6AP lowered β-catenin levels. Coexpression with E6 intensified the activities of E6AP. Further experiments proved that E6AP/E6 stabilize β-catenin by protecting it from proteasomal degradation. This function was dependent on the catalytic activity of E6AP, the kinase activity of GSK3β and the susceptibility of β-catenin to GSK3β phosphorylation. Thus, this study identified E6AP as a novel regulator of the Wnt signaling pathway, capable of cooperating with E6 in stimulating or augmenting Wnt/β-catenin signaling, thereby possibly contributing to HPV carcinogenesis. - Highlights: • The roles of E6 and E6AP in the Wnt pathway were investigated. • E6AP stabilizes E6 and enhances E6 activity in augmentation of Wnt signaling. • E6AP cooperates with E6 to stabilize β-catenin and stimulate Wnt/β-catenin signaling. • E6AP and E6 act through different mechanisms to augment or stimulate Wnt signaling.

  12. On the evolution of the magnetic field of Ap star $\\alpha^2$ CVn

    CERN Document Server

    Bychkov, V D; Madej, J; Topilskaya, G P

    2016-01-01

    New high-precision measurements of the longitudinal magnetic field of Ap stars suggest the existence of secular intrinsic variations of the global magnetic field in some stars. We argue that such changes are apparent in the Ap star $\\alpha^2$ CVn in the time scale of $\\sim$ 10 years, which results from the analysis of literature data. Therefore, such an observation implies, that the rate of magnetic field evolution of Ap stars is much higher than was previously thought.

  13. Research and project practice in AP1000 nuclear plant MCR assemblies seismic test

    International Nuclear Information System (INIS)

    Background: The seismic test is one of Plant Equipment Seismic Qualification Methodologies. Purpose: To satisfy the special requirements of AP1000 seismic qualification, this test is not same to traditional seismic test. Methods: AP1000 nuclear plant equipment qualification has new methods and requirements. The special requirements as response spectrum, accelerometer installation and functional test are described for AP1000 MCR seismic test. Results: The test results are demonstrated that MCR assemblies are satisfied as AP1000 seismic qualification requirements. Conclusions: These requirements are beneficial to the qualification for structural integrity and functional safety. They are also used to find design margin. (authors)

  14. Heterologous expression in Tritrichomonas foetus of functional Trichomonas vaginalis AP65 adhesin

    Directory of Open Access Journals (Sweden)

    Alderete JF

    2005-03-01

    Full Text Available Abstract Background Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs, a step preparatory to infection, is mediated in part by the prominent surface protein AP65. The bovine trichomonad, Tritrichomonas foetus, adheres poorly to human VECs. Thus, we established a transfection system for heterologous expression of the T. vaginalis AP65 in T. foetus, as an alternative approach to confirm adhesin function for this virulence factor. Results In this study, we show stable transfection and expression of the T. vaginalis ap65 gene in T. foetus from an episomal pBS-ap65-neo plasmid. Expression of the gene and protein was confirmed by RT-PCR and immunoblots, respectively. AP65 in transformed T. foetus bound to host cells. Specific mAbs revealed episomally-expressed AP65 targeted to the parasite surface and hydrogenosome organelles. Importantly, surface-expression of AP65 in T. foetus paralleled increased levels of adherence of transfected bovine trichomonads to human VECs. Conclusion The T. vaginalis AP65 adhesin was stably expressed in T. foetus, and the data obtained using this heterologous system strongly supports the role of AP65 as a prominent adhesin for T. vaginalis. In addition, the heterologous expression in T. foetus of a T. vaginalis gene offers an important, new approach for confirming and characterizing virulence factors.

  15. APS Goes to ComicCon and Other Tales: Reaching out for Physics

    Science.gov (United States)

    Kirby, Kate

    2012-10-01

    Broadly interpreting the core mission of APS ``to advance and diffuse the knowledge of physics", APS carries out programmatic activities in Education, Outreach, International Affairs, Public Affairs, and Advocacy, which benefit the physics community as well as society at large. These programs are highly valued by APS members who know about them, but the challenge has been to find ways to effectively communicate to our members about all the good things we are doing. APS programs have also achieved significant recognition in the external community for their outstanding quality and success. I will describe several examples of how we ``reach out" to promote physics and physics education in a variety of venues.

  16. AP Selection Algorithm for Real-Time Communications through Mixed WLAN Environments

    Science.gov (United States)

    Morioka, Yasufumi; Higashino, Takeshi; Tsukamoto, Katsutoshi; Komaki, Shozo

    Recent rapid development of high-speed wireless access technologies has created mixed WLAN (Wireless LAN) environments where QoS capable APs coexist with legacy APs. To provide QoS guarantee in this mixed WLAN environment, this paper proposes a new AP selection algorithm. The proposed algorithm assigns an STA (Station) to an AP in the overall WLAN service area. Simulation results show improvement in the VoIP performance in terms of an eMOS (estimated Mean Opinion Score) value and the FTP throughput compared to conventional algorithms.

  17. Isolation, classification and transcription profiles of the AP2/ERF transcription factor superfamily in citrus.

    Science.gov (United States)

    Xie, Xiu-lan; Shen, Shu-ling; Yin, Xue-ren; Xu, Qian; Sun, Chong-de; Grierson, Donald; Ferguson, Ian; Chen, Kun-song

    2014-07-01

    The AP2/ERF gene family encodes plant-specific transcription factors. In model plants, AP2/ERF genes have been shown to be expressed in response to developmental and environmental stimuli, and many function downstream of the ethylene, biotic, and abiotic stress signaling pathways. In citrus, ethylene is effective in regulation citrus fruit quality, such as degreening and aroma. However, information about the citrus AP2/ERF family is limited, and would enhance our understanding of fruit responses to environmental stress, fruit development and quality. CitAP2/ERF genes were isolated using the citrus genome database, and their expression patterns analyzed by real-time PCR using various orange organs and samples from a fruit developmental series. 126 sequences with homologies to AP2/ERF proteins were identified from the citrus genome, and, on the basis of their structure and sequence, assigned to the ERF family (102), AP2 family (18), RAV family (4) and Soloist (2). MEME motif analysis predicted the defining AP2/ERF domain and EAR repressor domains. Analysis of transcript accumulation in Citrus sinensis cv. 'Newhall' indicated that CitAP2/ERF genes show organ-specific and temporal expression, and provided a framework for understanding the transcriptional regulatory roles of AP2/ERF gene family members in citrus. Hierarchical cluster analysis and t tests identified regulators that potentially function during orange fruit growth and development. PMID:24566692

  18. Isolation, classification and transcription profiles of the AP2/ERF transcription factor superfamily in citrus.

    Science.gov (United States)

    Xie, Xiu-lan; Shen, Shu-ling; Yin, Xue-ren; Xu, Qian; Sun, Chong-de; Grierson, Donald; Ferguson, Ian; Chen, Kun-song

    2014-07-01

    The AP2/ERF gene family encodes plant-specific transcription factors. In model plants, AP2/ERF genes have been shown to be expressed in response to developmental and environmental stimuli, and many function downstream of the ethylene, biotic, and abiotic stress signaling pathways. In citrus, ethylene is effective in regulation citrus fruit quality, such as degreening and aroma. However, information about the citrus AP2/ERF family is limited, and would enhance our understanding of fruit responses to environmental stress, fruit development and quality. CitAP2/ERF genes were isolated using the citrus genome database, and their expression patterns analyzed by real-time PCR using various orange organs and samples from a fruit developmental series. 126 sequences with homologies to AP2/ERF proteins were identified from the citrus genome, and, on the basis of their structure and sequence, assigned to the ERF family (102), AP2 family (18), RAV family (4) and Soloist (2). MEME motif analysis predicted the defining AP2/ERF domain and EAR repressor domains. Analysis of transcript accumulation in Citrus sinensis cv. 'Newhall' indicated that CitAP2/ERF genes show organ-specific and temporal expression, and provided a framework for understanding the transcriptional regulatory roles of AP2/ERF gene family members in citrus. Hierarchical cluster analysis and t tests identified regulators that potentially function during orange fruit growth and development.

  19. K-AP: Generating specified K clusters by efficient Affinity Propagation

    KAUST Repository

    Zhang, Xiangliang

    2010-12-01

    The Affinity Propagation (AP) clustering algorithm proposed by Frey and Dueck (2007) provides an understandable, nearly optimal summary of a data set. However, it suffers two major shortcomings: i) the number of clusters is vague with the user-defined parameter called self-confidence, and ii) the quadratic computational complexity. When aiming at a given number of clusters due to prior knowledge, AP has to be launched many times until an appropriate setting of self-confidence is found. The re-launched AP increases the computational cost by one order of magnitude. In this paper, we propose an algorithm, called K-AP, to exploit the immediate results of K clusters by introducing a constraint in the process of message passing. Through theoretical analysis and experimental validation, K-AP was shown to be able to directly generate K clusters as user defined, with a negligible increase of computational cost compared to AP. In the meanwhile, K-AP preserves the clustering quality as AP in terms of the distortion. K-AP is more effective than k-medoids w.r.t. the distortion minimization and higher clustering purity. © 2010 IEEE.

  20. Study on the Transportation Organization Plan of Shenyang Metro Line 2 During the 12th National Games%沈阳地铁二号线运输组织方案优化研究--以第十二届全运会期间为例

    Institute of Scientific and Technical Information of China (English)

    刘小玲; 薛亮

    2014-01-01

    分析了沈阳地铁二号线断面客流的时空分布特征和运力匹配情况,提出了二号线在第十二届全运会期间存在的高峰期运力不足、上下行不均衡程度大等问题。根据全运会期间的客流特征,提出了全运会期间地铁二号线运输组织方案的优化研究。%With an analysis of the sectio n passenger flow distribution features of time space continuum, the matching condition between planning capacity and the actual transport volume of Shenyang metro Line 2, this article brings forward some potential problems of Line 2 during the 12th national games, such as the deficiency of train capacity in peak times, the serious imbalance of up and town traffic, and so on. The op-timization research on transportation organization plan of Line 2 during 2013 national games is presented according to the passenger character during the national games.

  1. Toxic epidermal necrolysis related to AP (pemetrexed plus cisplatin) and gefitinib combination therapy in a patient with metastatic non-small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    Ji-Jie Huang; Shu-Xiang Ma; Xue Hou; Zhao Wang; Yin-Duo Zeng; Tao Qin; Xiao-Xiao Dinglin; Li-Kun Chen

    2015-01-01

    Toxic epidermal necrolysis (TEN) is a rare acute life-threatening mucocutaneous disorder that is mostly drug-related (80%-95%). It is clinical y characterized as a widespread sloughing of the skin and mucosa. AP regimen (pemetrexed plus cisplatin) has been the preferred first-line chemotherapy for metastatic non-squamous non-small cell lung cancer (NSCLC). Gefitinib, a small-molecule epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), has already been recommended as a first-line treatment in EGFR-mutant metastatic NSCLC. We report rare presentation of TEN involving adverse effects of AP and gefitinib combination treatment in a 42-year-old woman diagnosed with metastatic NSCLC harboring an EGFR mutation. On the 21st day after administration of the first cycle of AP regimen and the 8th day after the initiation of gefitinib treatment, she developed an acne-like rash, oral ulcer, and conjunctivitis, which later became blisters and ultimately denuded. The characteristic clinical courses were decisive for the diagnosis of TEN. Treatment with systemic steroids and immunoglobulin as well as supportive treatment led to an improvement of her general condition and a remarkable recovery.

  2. LBB在AP1000技术中的应用%The Application of LBB in AP1000 Technology

    Institute of Scientific and Technical Information of China (English)

    蒋冬梅; 杜颖; 袁小兰

    2015-01-01

    压水堆核电厂的设计基准事故定义为主管道双端断裂,这一假设过于保守.管道的失效方式往往是先出现破口而后再泄漏,即所谓的先漏后破( Leak Before Break,简称LBB) ,而不是双端断裂. LBB准则是防止核电厂压力管道发生灾难性破裂事故的重要评定准则.本文介绍了LBB评定技术在国外的发展情况和意义,阐述了LBB在AP1000核电厂中的应用情况及LBB评价的理论基础.%Design basis accident of pressurized water reactor nuclear power plant was de-fined as main pipeline double-end fracture, this assumption is too conservative. Pipeline failure modes often appear as first Break then Leak,that is so-called the Leak Before Break ( Leak Before Break,LBB) ,rather than double end fracture. LBB is an important evalua-tion criteria of preventing pressure piping catastrophic rupture accident of nuclear power plant . This paper briefly introduces the development situation and the significance of the o-verseas LBB assessment technology and expounds the application of LBB in AP1000 nucle-ar power plant and the theoretical basis of LBB assessment.

  3. Analysis of human reliability in the APS of fire. Application of NUREG-1921; Analisis de Fiabilidad Humana en el APS de Incendios. Aplicacion del NUREG-1921

    Energy Technology Data Exchange (ETDEWEB)

    Perez Torres, J. L.; Celaya Meler, M. A.

    2014-07-01

    An analysis of human reliability in a probabilistic safety analysis (APS) of fire aims to identify, describe, analyze and quantify, in a manner traceable, human actions that can affect the mitigation of an initiating event produced by a fire. (Author)

  4. Neurotransmitter transporters

    DEFF Research Database (Denmark)

    Gether, Ulrik; Andersen, Peter H; Larsson, Orla M;

    2006-01-01

    The concentration of neurotransmitters in the extracellular space is tightly controlled by distinct classes of membrane transport proteins. This review focuses on the molecular function of two major classes of neurotransmitter transporter that are present in the cell membrane of neurons and....../or glial cells: the solute carrier (SLC)1 transporter family, which includes the transporters that mediate the Na(+)-dependent uptake of glutamate, and the SLC6 transporter family, which includes the transporters that mediate the Na(+)-dependent uptake of dopamine, 5-HT, norepinephrine, glycine and GABA....... Recent research has provided substantial insight into the structure and function of these transporters. In particular, the recent crystallizations of bacterial homologs are of the utmost importance, enabling the first reliable structural models of the mammalian neurotransmitter transporters...

  5. Pseudoephedrine inhibits T-cell activation by targeting NF-κB, NFAT and AP-1 signaling pathways.

    Science.gov (United States)

    Fiebich, Bernd L; Collado, Juan A; Stratz, Cristian; Valina, Christian; Hochholzer, Willibald; Muñoz, Eduardo; Bellido, Luz M

    2012-02-01

    Pseudoephedrine (PSE) is a stereoisomer of ephedrine that is commonly used as a nasal decongestant in combination with other anti-inflammatory drugs for the symptomatic treatment of some common pathologies such as common cold. Herein, we describe for the first time the effects of PSE on T-cell activation events. We found that PSE inhibits interleukin-2 (IL-2) and tumor necrosis factor (TNF) alpha-gene transcription in stimulated Jurkat cells, a human T-cell leukemia cell line. To further characterize the inhibitory mechanisms of PSE at the transcriptional level, we examined the transcriptional activities of nuclear factor kappa B (NF-κB), nuclear factor of activated T cells (NFAT), and activator protein-1 (AP-1) transcription factors and found that PSE inhibited NF-κB-dependent transcriptional activity without affecting either the phosphorylation, the degradation of the cytoplasmic NF-κB inhibitory protein, IκBα or the DNA-binding activity. However, phosphorylation of the p65/RelA subunit was clearly inhibited by PSE in stimulated cells. In addition, PSE inhibited the transcriptional activity of NFAT without interfering with the calcium-induced NFAT dephosphorylation event, which represents the major signaling pathway for its activation. NFAT cooperates with c-Jun, a compound of the AP-1 complex, to activate target genes, and we also found that PSE inhibited both JNK activation and AP-1 transcriptional activity. These findings provide new mechanistic insights into the potential immunomodulatory activities of PSE and highlight their potential in designing novel therapeutic strategies to manage inflammatory diseases.

  6. Radioactive air emissions notice of construction for installation and operation of a waste retrieval system and tanks 241-AP-102 and 241-AP-104 project

    Energy Technology Data Exchange (ETDEWEB)

    DEXTER, M.L.

    1999-11-15

    This document serves as a notice of construction (NOC) pursuant to the requirements of Washington Administrative Code (WAC) 246 247-060, and as a request for approval to modify pursuant to 40 Code of Federal Regulations (CFR) 61 07 for the installation and operation of one waste retrieval system in the 24 1 AP-102 Tank and one waste retrieval system in the 241 AP 104 Tank Pursuant to 40 CFR 61 09 (a)( 1) this application is also intended to provide anticipated initial start up notification Its is requested that EPA approval of this application will also constitute EPA acceptance of the initial start up notification Project W 211 Initial Tank Retrieval Systems (ITRS) is scoped to install a waste retrieval system in the following double-shell tanks 241-AP 102-AP 104 AN 102, AN 103, AN-104, AN 105, AY 102 AZ 102 and SY-102 between now and the year 2011. Because of the extended installation schedules and unknowns about specific activities/designs at each tank, it was decided to submit NOCs as that information became available This NOC covers the installation and operation of a waste retrieval system in tanks 241 AP-102 and 241 AP 104 Generally this includes removal of existing equipment installation of new equipment and construction of new ancillary equipment and buildings Tanks 241 AP 102 and 241 AP 104 will provide waste feed for immobilization into a low activity waste (LAW) product (i.e. glass logs) The total effective dose equivalent (TEDE) to the offsite maximally exposed individual (MEI) from the construction activities is 0 045 millirem per year The unabated TEDE to the offsite ME1 from operation of the mixer pumps is 0 042 millirem per year.

  7. Radioactive air emissions notice of construction for installation and operation of a waste retrieval system and tanks 241-AP-102 and 241-AP-104 project

    International Nuclear Information System (INIS)

    This document serves as a notice of construction (NOC) pursuant to the requirements of Washington Administrative Code (WAC) 246 247-060, and as a request for approval to modify pursuant to 40 Code of Federal Regulations (CFR) 61 07 for the installation and operation of one waste retrieval system in the 24 1 AP-102 Tank and one waste retrieval system in the 241 AP 104 Tank Pursuant to 40 CFR 61 09 (a)( 1) this application is also intended to provide anticipated initial start up notification Its is requested that EPA approval of this application will also constitute EPA acceptance of the initial start up notification Project W 211 Initial Tank Retrieval Systems (ITRS) is scoped to install a waste retrieval system in the following double-shell tanks 241-AP 102-AP 104 AN 102, AN 103, AN-104, AN 105, AY 102 AZ 102 and SY-102 between now and the year 2011. Because of the extended installation schedules and unknowns about specific activities/designs at each tank, it was decided to submit NOCs as that information became available This NOC covers the installation and operation of a waste retrieval system in tanks 241 AP-102 and 241 AP 104 Generally this includes removal of existing equipment installation of new equipment and construction of new ancillary equipment and buildings Tanks 241 AP 102 and 241 AP 104 will provide waste feed for immobilization into a low activity waste (LAW) product (i.e. glass logs) The total effective dose equivalent (TEDE) to the offsite maximally exposed individual (MEI) from the construction activities is 0 045 millirem per year The unabated TEDE to the offsite ME1 from operation of the mixer pumps is 0 042 millirem per year

  8. School Transportation.

    Science.gov (United States)

    Executive Educator, 1990

    1990-01-01

    This special section on student transportation offers a case study of a school system that recycles buses for safety drills; articles on fuel-saving strategies, the pros and cons of contracting for transportation services or operating a publicly owned bus fleet, and advice on full cost accounting for transportation costs; and a transportation…

  9. A Review on the Change of the International Transport Line to China by the National Government during the War of Resistance against Japan%抗战时期国民政府国际援华运输线路变迁述评

    Institute of Scientific and Technical Information of China (English)

    韩继伟

    2012-01-01

    全面抗战爆发后,国民政府迁都西南。为保证前线之军需和稳定大后方之经济,开辟和保持国际运输线路,从国外获取必须的物资补给,是当时极为重要的战略目标之一。随着战争形势与国际关系的变化,国民政府国际运输线路也不断地进行变更。保持国际运输线路的通畅,打破日军的国际交通战略封锁,是当时国民政府的战略任务之一;同时,也符合世界反法西斯国家的联盟战略,在我国抗战史上占有重要的一页。%After the Sino - Japanese War broke out, the then national government moved to the southwest. It was one of the most important strategic targets to ensure the military needs at the front and the economic stability in the rear area, to open up and maintain the international transport lines so as to obtain the necessary supplies from abroad. With the change of the war situation and international relationship, the international transport line was also changed time and again by the then national government. It was one of their strategic tasks to keep the international transport routes clear and to break the strategic blockade of the line imposed by Japanese invaders. This strategy conformed to the alliance one to go against the fascist countries in the world, and occupied an important page in the history of the Anti -Japanese War.

  10. 49 CFR 192.709 - Transmission lines: Record keeping.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Transmission lines: Record keeping. 192.709... TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Maintenance § 192.709 Transmission lines: Record keeping. Each operator shall maintain the following records for transmission...

  11. 49 CFR 179.201-3 - Lined tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Lined tanks. 179.201-3 Section 179.201-3... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.201-3 Lined tanks. (a)...

  12. TinyCoAP: A Novel Constrained Application Protocol (CoAP Implementation for Embedding RESTful Web Services in Wireless Sensor Networks Based on TinyOS

    Directory of Open Access Journals (Sweden)

    Anna Calveras

    2013-05-01

    Full Text Available In this paper we present the design and implementation of the Constrained Application Protocol (CoAP for TinyOS, which we refer to as TinyCoAP. CoAP seeks to apply the same application transfer paradigm and basic features of HTTP to constrained networks, while maintaining a simple design and low overhead. The design constraints of Wireless Sensor Networks (WSNs require special attention in the design process of the CoAP implementation. We argue that better performance and minimal resource consumption can be achieved developing a native library for the operating system embedded in the network. TinyOS already includes in its distribution an implementation of CoAP called CoapBlip. However, this is based on a library not originally designed to meet the requirements of TinyOS. We demonstrate the effectiveness of our approach by a comprehensive performance evaluation. In particular, we test and evaluate TinyCoAP and CoapBlip in a real scenario, as well as solutions based on HTTP. The evaluation is performed in terms of latency, memory occupation, and energy consumption. Furthermore, we evaluate the reliability of each solution by measuring the goodput obtained in a channel affected by Rayleigh fading. We also include a study on the effects that high workloads have on a server.

  13. Transient Fcho1/2⋅Eps15/R⋅AP-2 Nanoclusters Prime the AP-2 Clathrin Adaptor for Cargo Binding.

    Science.gov (United States)

    Ma, Li; Umasankar, Perunthottathu K; Wrobel, Antoni G; Lymar, Anastasia; McCoy, Airlie J; Holkar, Sachin S; Jha, Anupma; Pradhan-Sundd, Tirthadipa; Watkins, Simon C; Owen, David J; Traub, Linton M

    2016-06-01

    Clathrin-coated vesicles form by rapid assembly of discrete coat constituents into a cargo-sorting lattice. How the sequential phases of coat construction are choreographed is unclear, but transient protein-protein interactions mediated by short interaction motifs are pivotal. We show that arrayed Asp-Pro-Phe (DPF) motifs within the early-arriving endocytic pioneers Eps15/R are differentially decoded by other endocytic pioneers Fcho1/2 and AP-2. The structure of an Eps15/R⋅Fcho1 μ-homology domain complex reveals a spacing-dependent DPF triad, bound in a mechanistically distinct way from the mode of single DPF binding to AP-2. Using cells lacking FCHO1/2 and with Eps15 sequestered from the plasma membrane, we establish that without these two endocytic pioneers, AP-2 assemblies are fleeting and endocytosis stalls. Thus, distinct DPF-based codes within the unstructured Eps15/R C terminus direct the assembly of temporary Fcho1/2⋅Eps15/R⋅AP-2 ternary complexes to facilitate conformational activation of AP-2 by the Fcho1/2 interdomain linker to promote AP-2 cargo engagement. PMID:27237791

  14. Preface: 18th Aps-Sccm and 24th Airapt

    Science.gov (United States)

    Collins, Gilbert; Moore, David S.; Yoo, Choong-Shik; Buttler, William; Furlanetto, Michael; Evans, William

    2014-05-01

    The 18th Biennial International Conference of the APS Topical Group on Shock Compression of Condensed Matter in conjunction with the 24th Biennial International Conference of the International Association for the Advancement of High Pressure Science & Technology (AIRAPT) was held at the Westin Hotel in Seattle, Washington from 7-12 July, 2013. This is only the second time that these two organizations have held a Joint Conference — the first was 20 years previous (1993) in Colorado Springs, Colorado. Seattle was chosen for this joint conference because of its central location for the world-wide attendees as well as its metropolitan vibrancy. The scientific program consisted of 858 scheduled presentations organized into 23 topical areas and included contributed (537), invited (95), and plenary (6) lectures, as well as two poster sessions with 110 posters each. The scientific focus of the Joint Conference was on fundamental and applied research topics related to the static or dynamic compression of condensed matter. This multidisciplinary field of research encompasses areas of physics, chemistry, materials science, mechanics, geophysics and planetary physics, and applied mathematics. Experimental, computational and theoretical studies all play important roles. The organizers endeavored to intertwine static and dynamic experimental alongside computational and theoretical studies of similar materials in the organization of the sessions. This goal was aided by the addition of three special focus sessions on deep carbon budget, high energy density materials, and dynamic response of materials. 722 scientists and engineers from 25 countries registered at the conference, including 132 students from 12 countries. The attendee countries represented included: Argentina (2), Australia (2), Brazil (3), Canada (25), China (22), Czech Republic (2), France (35), Germany (19), India (6), Israel (21), Italy (10), Japan (49), Netherlands (1), Poland (1), Portugal (2), Russia (26

  15. Conceptual design report for the UNI-CAT beam line proposal

    International Nuclear Information System (INIS)

    The overall thrusts of UNI-CAT (University-National Laboratory- Industry Collaborative Access Team) are research at the cutting edge of physics, chemistry, biology, materials science, chemical engineering, polymer science, and geology; and education of a new generation of scientists in the use of synchrotron radiation to probe the structure, chemistry, and dynamic behavior of materials. The UNI-CAT proposal is to develop an APS sector that includes a bending magnet beam line and an insertion device beam line. The APS type-A undulator is the preferred choice for the insertion device. 8 figs, 6 refs

  16. Beam-line considerations for experiments with highly-charged ions

    International Nuclear Information System (INIS)

    The APS offers exciting possibilities for a bright future in x-ray research. For example, measurements on the inner-shell photoionization of ions will be feasible using stored ions in ion traps or ion beams from an electron-cyclotron-resonance ion source, or perhaps even a heavy-ion storage ring. Such experiments with ionic targets are the focus for the APS. The performance of beam lines X26C, X26A, and X17 on the x-ray ring of the National Synchrotron Light Source will be discussed as specific examples of beam-line design considerations

  17. Magnetic AP Stars in the Hertzsprung-Russell Diagram

    Science.gov (United States)

    Hubrig, S.; North, P.; Mathys, G.

    2000-08-01

    The evolutionary state of magnetic Ap stars is rediscussed using the recently released Hipparcos data. The distribution of the magnetic Ap stars of mass below 3 Msolar in the H-R diagram differs from that of the normal stars in the same temperature range at a high level of significance. Magnetic stars are concentrated toward the center of the main-sequence band. This is shown in two forms of the H-R diagram: one where logL is plotted against logTeff and a version more directly tied to the observed quantities, showing the astrometry-based luminosity (Arenou & Luri) against the (B2-G)0 index of Geneva photometry. In particular, it is found that magnetic fields appear only in stars that have already completed at least approximately 30% of their main-sequence lifetime. No clear picture emerges as to the possible evolution of the magnetic field across the main sequence. Hints of some (loose) relations between magnetic field strength and other stellar parameters are found: stars with shorter periods tend to have stronger fields, as do higher temperature and higher mass stars. A marginal trend of the magnetic flux to be lower in more slowly rotating stars may possibly be seen as suggesting a dynamo origin for the field. No correlation between the rotation period and the fraction of the main-sequence lifetime completed is observed, indicating that the slow rotation in these stars must already have been achieved before they became observably magnetic. Based on data from the ESA Hipparcos satellite and on observations collected at the European Southern Observatory (La Silla, Chile; ESO programs Nos. 43.7-004, 44.7-012, 49.7-030, 50.7-067, 51.7-041, 52.7-063, 53.7-028, 54.E-0416, and 55.E-0751), at the Observatoire de Haute-Provence (Saint-Michel l'Observatoire, France), at Kitt Peak National Observatory, and at the Canada-France-Hawaii Telescope.

  18. The AP-1 Transcription Factor c-Jun Prevents Stress-Imposed Maladaptive Remodeling of the Heart

    Science.gov (United States)

    Windak, Renata; Müller, Julius; Felley, Allison; Akhmedov, Alexander; Wagner, Erwin F.; Pedrazzini, Thierry; Sumara, Grzegorz; Ricci, Romeo

    2013-01-01

    Systemic hypertension increases cardiac workload and subsequently induces signaling networks in heart that underlie myocyte growth (hypertrophic response) through expansion of sarcomeres with the aim to increase contractility. However, conditions of increased workload can induce both adaptive and maladaptive growth of heart muscle. Previous studies implicate two members of the AP-1 transcription factor family, junD and fra-1, in regulation of heart growth during hypertrophic response. In this study, we investigate the function of the AP-1 transcription factors, c-jun and c-fos, in heart growth. Using pressure overload-induced cardiac hypertrophy in mice and targeted deletion of Jun or Fos in cardiomyocytes, we show that c-jun is required for adaptive cardiac hypertrophy, while c-fos is dispensable in this context. c-jun promotes expression of sarcomere proteins and suppresses expression of extracellular matrix proteins. Capacity of cardiac muscle to contract depends on organization of principal thick and thin filaments, myosin and actin, within the sarcomere. In line with decreased expression of sarcomere-associated proteins, Jun-deficient cardiomyocytes present disarrangement of filaments in sarcomeres and actin cytoskeleton disorganization. Moreover, Jun-deficient hearts subjected to pressure overload display pronounced fibrosis and increased myocyte apoptosis finally resulting in dilated cardiomyopathy. In conclusion, c-jun but not c-fos is required to induce a transcriptional program aimed at adapting heart growth upon increased workload. PMID:24039904

  19. Transport in graphene antidot barriers and tunneling devices

    OpenAIRE

    Pedersen, Thomas Garm; Pedersen, Jesper Goor

    2012-01-01

    Periodic arrays of antidots, i.e. nanoscale perforations, in graphene enable tight confinement of carriers and efficient transport barriers. Such barriers evade the Klein tunneling mechanism by being of the mass rather than electrostatic type. While all graphene antidot lattices (GALs) may support directional barriers, we show, however, that a full transport gap exists only for certain orientations of the GAL. Moreover, we assess the applicability of gapped graphene and the Dirac continuum ap...

  20. Understanding and Using the New Guided-Inquiry AP Chemistry Laboratory Manual

    Science.gov (United States)

    Cacciatore, Kristen L.

    2014-01-01

    To support teaching and learning in the advanced placement (AP) chemistry laboratory, the College Board published a laboratory manual, "AP Chemistry Guided-Inquiry Experiments: Applying the Science Practices," in 2013 as part of the redesigned course. This article provides a discussion of the rationale for the existence of the manual as…