WorldWideScience

Sample records for aps transport lines

  1. AP stars with resolved Zeeman split lines

    Science.gov (United States)

    Mathys, G.

    1990-06-01

    High-resolution, high SNR observations of a sample of sharp-lined A stars and of Ap stars showing resolved Zeeman split lines are presented. The Fe II lines 6147.7 A and 6149.2 A unexpectedly appear to be asymmetric in all stars where they are resolved. The blue component of the 6149.2 line, which is a Zeeman doublet, is deeper and narrower than its red component. For line 6147.7, whose Zeeman pattern does not differ much from a quadruplet, the red components are deeper than the blue ones. It is shown that a partial Paschen-Back effect can account for these properties. The potential implications of this finding for studies of magnetic Ap stars are discussed.

  2. Some magnetic null lines of astrophysical interest. II. [in magnetic Ap stars

    Science.gov (United States)

    Adelman, S. J.

    1974-01-01

    A list of magnetic null lines from selected spectroscopic analyses is presented for possible use in detailed studies of magnetic Ap stars. The presented data represent an extension of the magnetic null lines whose usefulness Shore and Adelman (1974) have recently demonstrated for the study of the overall physical conditions prevailing in the atmospheres of peculiar A stars and for testing the consequences of the mechanism of selective elemental diffusion.

  3. Beam chopper For the Low-Energy Undulator Test Line (LEUTL) in the APS

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Y.; Wang, J.; Milton, S.; Teng, L. [and others

    1997-08-01

    The low-energy undulator test line (LEUTL) is being built and will be tested with a short beam pulse from an rf gun in the Advanced Photon Source (APS) at the Argonne National Laboratory. In the LEUTL a beam chopper is used after the rf gun to deflect the unwanted beam to a beam dump. The beam chopper consists of a permanent magnet and an electric deflector that can compensate for the magnetic deflection. A 30-kV pulsed power supply is used for the electric deflector. The chopper subsystem was assembled and tested for beamline installation. The electrical and beam properties of the chopper assembly are presented.

  4. Evidence for Magnetar Formation in Broad-lined Type Ic Supernovae 1998bw and 2002ap

    Science.gov (United States)

    Wang, L. J.; Yu, H.; Liu, L. D.; Wang, S. Q.; Han, Y. H.; Xu, D.; Dai, Z. G.; Qiu, Y. L.; Wei, J. Y.

    2017-03-01

    Broad-lined type Ic supernovae (SNe Ic-BL) are peculiar stellar explosions that are distinct from ordinary SNe. Some SNe Ic-BL are associated with long-duration (≳ 2 {{s}}) gamma-ray bursts (GRBs). Black holes and magnetars are two types of compact objects that are hypothesized to be central engines of GRBs. In spite of decades of investigations, no direct evidence for the formation of black holes or magnetars has yet been found for GRBs. Here we report the finding that the early peak (t≲ 50 {days}) and late-time (t≳ 300 {days}) slow decay displayed in the light curves of SNe 1998bw (associated with GRB 980425) and 2002ap (not GRB-associated) can be attributed to magnetar spin-down with an initial rotation period {P}0∼ 20 {ms}, while the intermediate-time (50≲ t≲ 300 {days}) exponential decline is caused by the radioactive decay of 56Ni. The connection between the early peak and late-time slow decline in the light curves is unexpected in alternative models. We thus suggest that GRB 980425 and SN 2002ap were powered by magnetars.

  5. Analysis and Verification of Direct Vessel Injection Line Break event tree for AP1000 reactor with TRACE code

    Energy Technology Data Exchange (ETDEWEB)

    Queral, C.; Montero-Mayorga, J.; Gonzalez-Cadelo, J.

    2013-07-01

    The AP1000 PRA thermal hydraulic simulations were performed with MAAP code, which allows simulating sequences with low computational efforts. On the other hand, the use of best estimate codes allows verifying PRA results as well as obtaining a greater knowledge of the phenomenology of such sequences. The initiating event with the greatest contribution to core damage is Direct Vessel Injection Line Break (DVILB). This paper presents a review of DVILB sequences of AP1000 with TRACE code for verifying sequences previously analyzed by Westinghouse with MAAP code. The sequences which configure the DVILB event tree during short term have been simulated. The results obtained confirm the ones obtained in AP1000 PRA.

  6. BEAM TRANSPORT LINES FOR THE BSNS.

    Energy Technology Data Exchange (ETDEWEB)

    WEI, J.

    2006-06-26

    This paper presents the design of two beam transport lines at the BSNS: one is the injection line from the Linac to the RCS and the other is the target line from the RCS to the target station. In the injection beam line, space charge effects, transverse halo collimation, momentum tail collimation and debunching are the main concerned topics. A new method of using triplet cells and stripping foils is used to collimate transverse halo. A long straight section is reserved for the future upgrading linac and debuncher. In the target beam line, large halo emittance, beam stability at the target due to kicker failures and beam jitters, shielding of back-scattering neutrons from the target are main concerned topics. Special bi-gap magnets will be used to reduce beam losses in the collimators in front of the target.

  7. Evidence for magnetar formation in broad-lined type Ic supernovae 1998bw and 2002ap

    CERN Document Server

    Wang, L J; Liu, L D; Wang, S Q; Han, Y H; Xu, D; Dai, Z G; Qiu, Y L; Wei, J Y

    2016-01-01

    Broad-lined type Ic supernovae (SNe Ic-BL) are peculiar stellar explosions that distinguish themselves from ordinary SNe. Some SNe Ic-BL are associated with long-duration (\\gtrsim 2 s) gamma-ray bursts (GRBs). Black holes and magnetars are two types of compact objects that are hypothesized to be central engines of GRBs. In spite of decades of investigations, no direct evidence for the formation of black holes or magnetars has been found for GRBs so far. Here we report the discovery that the early peak (t \\lesssim 50 days) and late-time (t \\gtrsim 300 days) slow decay displayed in the light curves of both SNe 1998bw (associated with GRB 980425) and 2002ap (not GRB-associated) can be attributed to magnetar spin-down with initial rotation period P0 \\sim 20 ms, while the intermediate-time (50 \\lesssim t \\lesssim 300 days) linear decline is caused by radioactive decay of 56Ni. The connection between the early peak and late-time slow decline in the light curves is unexpected in alternative models. We thus suggest t...

  8. Waterborne Transportation Lines of the United States

    Science.gov (United States)

    1988-10-01

    JUNCTION Ny 12533 32! GANES TO0615 COMPANT 4419 OOLUARD DR. APT. O n SEA ORLEANS LU 70114 "I1 GEN A CACRICAL Cl. I QC EAST bALlET RIMD I A NE OP. TZO I I...1911 91I 339 9494090x. bay" , p. 0;.* 0019 I 9 ;’ 4010 00 7 96 31------- TAdtE - ALPNA& ETICA LIST OF TRANSPORTATION LINES- - - -. E4G. OPERATOR NAME A...200009 ".0! 19.9 I 00ISM00 9 Ss 0019 9644707 9 9 484 200.09 30.09 9.09 - 9 1500 14.99iON 9L6 GEN 1 9 9 9 9 9 200.01 00 9.9! 9 - 9 9 9 0RLEAN S8 5098

  9. Calibrating transport lines using LOCO techniques

    Energy Technology Data Exchange (ETDEWEB)

    Yves Roblin

    2011-09-01

    With the 12GeV upgrade underway at CEBAF, there is a need to re-characterize the beamlines after the modifications made to it to accommodate running at higher energies. We present a linear perturbation approach to calibrating the optics model of transport lines. This method is adapted from the LOCO method in use for storage rings. We consider the effect of quadrupole errors, dipole construction errors as well as beam position monitors and correctors calibrations. The ideal model is expanded to first order in Taylor series of the quadrupole errors. A set of difference orbits obtained by exciting the correctors along the beamline is taken, yielding the measured response matrix. An iterative procedure is invoked and the quadrupole errors as well as beam position monitors and corrector calibration factors are obtained. Here we present details of the method and results of first measurements at CEBAF in early 2011.

  10. Transport of the cholera toxin B-subunit from recycling endosomes to the Golgi requires clathrin and AP-1.

    Science.gov (United States)

    Matsudaira, Tatsuyuki; Niki, Takahiro; Taguchi, Tomohiko; Arai, Hiroyuki

    2015-08-15

    The retrograde pathway is defined by the transport of proteins and lipids from the plasma membrane through endosomes to the Golgi complex, and is essential for a variety of cellular activities. Recycling endosomes are important sorting stations for some retrograde cargo. SMAP2, a GTPase-activating protein (GAP) for Arf1 with a putative clathrin-binding domain, has previously been shown to participate in the retrograde transport of the cholera toxin B-subunit (CTxB) from recycling endosomes. Here, we found that clathrin, a vesicle coat protein, and clathrin adaptor protein complex 1 (AP-1) were present at recycling endosomes and were needed for the retrograde transport of CTxB from recycling endosomes to the Golgi, but not from the plasma membrane to recycling endosomes. SMAP2 immunoprecipitated clathrin and AP-1 through a putative clathrin-binding domain and a CALM-binding domain, and SMAP2 mutants that did not interact with clathrin or AP-1 could not localize to recycling endosomes. Moreover, knockdown of Arf1 suppressed the retrograde transport of CTxB from recycling endosomes to the Golgi. These findings suggest that retrograde transport is mediated by clathrin-coated vesicles from recycling endosomes and that the role of the coat proteins is in the recruitment of Arf GAP to transport vesicles.

  11. Development and evaluation of human AP endonuclease inhibitors in melanoma and glioma cell lines

    DEFF Research Database (Denmark)

    Mohammed, M Z; Vyjayanti, V N; Laughton, C A

    2011-01-01

    Modulation of DNA base excision repair (BER) has the potential to enhance response to chemotherapy and improve outcomes in tumours such as melanoma and glioma. APE1, a critical protein in BER that processes potentially cytotoxic abasic sites (AP sites), is a promising new target in cancer. In the...

  12. Breeding of Selectable Marker-Free Transgenic Rice Lines Containing AP1 Gene with Enhanced Disease Resistance

    Institute of Scientific and Technical Information of China (English)

    YU Heng-xiu; LIU Qiao-quan; WANG Ling; ZHAO Zhi-peng; XU Li; HUANG Ben-li; GONG Zhi-yun; TANG Shu-zhu; GU Ming-hong

    2006-01-01

    In order to obtain marker-free transgenic rice with improved disease resistance, the AP1 gene of Capsicum annuum and hygromycin-resistance gene (HPT) were cloned into the two separate T-DNA regions of the binary vector pSB 130,respectively, and introduced into the calli derived from the immature seeds of two elite japonica rice varieties, Guangling Xiangjing and Wuxiangjing 9, mediated by Agrobacterium-mediated transformation. Many cotransgenic rice lines containing both the AP1 gene and the marker gene were regenerated and the integration of both transgenes in the transgenic rice plants was confirmed by either PCR or Southern blotting technique. Several selectable marker-free transgenic rice plants were subsequently obtained from the progeny of the cotransformants, and confirmed by both PCR and Southern blotting analysis. These transgenic rice lines were tested in the field and their resistance to disease was c arefully investigated, the results showed that after inoculation the resistance to either bacterial blight or sheath blight of the selected transgenic lines was improved when compared with those of wild type.

  13. Interaction Between The Broad-lined Type Ic Supernova 2012ap and Carriers of Diffuse Interstellar Bands

    CERN Document Server

    Milisavljevic, D; Crabtree, K N; Foster, J B; Soderberg, A M; Fesen, R A; Parrent, J T; Sanders, N E; Drout, M R; Kamble, A; Chakraborti, S; Pickering, T E; Cenko, S B; Silverman, J M; Filippenko, A V; Kirshner, R P; Mazzali, P; Maeda, K; Marion, G H; Vinko, J; Wheeler, J C

    2014-01-01

    The diffuse interstellar bands (DIBs) are absorption features observed in optical and near-infrared spectra that are thought to be associated with carbon-rich polyatomic molecules in interstellar gas. However, because the central wavelengths of these bands do not correspond with electronic transitions of any known atomic or molecular species, their nature has remained uncertain since their discovery almost a century ago. Here we report on unusually strong DIBs in optical spectra of the broad-lined Type Ic supernova SN 2012ap that exhibit changes in equivalent width over short (~30 days) timescales. The 4428 and 6283 Angstrom DIB features get weaker with time, whereas the 5780 Angstrom feature shows a marginal increase. These nonuniform changes suggest that the supernova is interacting with a nearby source of the DIBs and that the DIB carriers possess high ionization potentials, such as small cations or charged fullerenes. We conclude that moderate-resolution spectra of supernovae with DIB absorptions obtained...

  14. The Paschen-Back effect in the Li I 6708 Å line and the presence of lithium in cool magnetic Ap stars

    Science.gov (United States)

    Kochukhov, O.

    2008-05-01

    Context: A number of cool magnetic Ap stars show a prominent feature at λ 6708 Å. Its identification with Li I remains controversial due to the lack of knowledge of the spectra of rare-earth elements that are strongly enhanced in peculiar stars so they can potentially provide an alternative identification. Aims: We suggest investigating the 6708 Å line in Ap stars with strong magnetic fields. In these objects, the magnetic broadening and splitting provides an additional, powerful criterium for line identification, allowing the whole line profile to be used instead of depending on a mere coincidence in the observed and predicted wavelengths. Methods: The small separation of the Li I doublet components means that their magnetic splitting pattern deviates from the one expected for the Zeeman effect, even in relatively weak fields. We carried out detailed calculations of the transition between the Zeeman and Paschen-Back regimes in the magnetic splitting of the Li I line and computed polarised synthetic spectra for the range of field strength expected in Ap stars. Theoretical spectral synthesis is compared with the high-resolution observations of cool Ap stars HD 116114, HD 166473, and HD 154708, which have a mean field strength of 6.4, 8.6, and 24.5 kG, respectively, and show a strong 6708 Å line. Results: High-resolution spectra for the 6708 Å region were analysed for 17 magnetic Ap stars. The presence of the 6708 Å line is confirmed for 9 stars and reported for the first time for 6 stars. The strength of the Li I doublet does not correlate with the absorption features of any other element. The stars HD 75445 and HD 201601 provide an extreme example of the two objects, which are dissimilar with respect to the 6708 Å line, but very close in the atmospheric parameters and abundances of other elements. We demonstrate that the observed profiles of the 6708 Å line in the strong field stars HD 116114, HD 166473, and HD 154708 correspond fairly well to the

  15. Transient Characteristics of Main Feedwater Line Rupture Accident for AP1000 Nuclear Power Plant%AP1000核电厂主给水管道断裂事故瞬态特性分析

    Institute of Scientific and Technical Information of China (English)

    贾祥; 安婕铷; 靖剑平

    2016-01-01

    AP1000是目前国际上典型的“三代”非能动核电厂,基于最佳估算程序 RELAP5/MOD3.3,对AP1000核电厂系统进行了详细的建模分析,获得了主给水管道断裂事故下 AP1000核电厂关键参数的瞬态特性和非能动系统响应特性。结果表明,事故过程中一、二回路的压力和温度呈现波动变化,一回路压力最大值为17.13 MPa,低于设计压力的91%,主蒸汽系统的压力也低于设计值的91%,满足验收准则的要求。%The AP1000 is the typical “third generation”passive nuclear power plant in the world at present.The primary system of AP1000 nuclear power plant was modeled using RELAP5/MOD3.3 code,and the transient thermal-hydraulic characteristics and the response characteristics of passive system were analyzed under the accident sequence of main feedwater line rupture accident.The results show that during the accident,the primary loop pressure,secondary loop pressure and primary loop temperature are fluc-tuant.The RCS pressure maximum value is 17.13 MPa,less than 91% of the design pressure.The pressure of the main steam system is also less than 91% of the designed value,which satisfies acceptance criteria.

  16. The Paschen-Back effect in the Li I 6708 A line and the presence of lithium in cool magnetic Ap stars

    CERN Document Server

    Kochukhov, O

    2008-01-01

    A number of cool magnetic Ap stars show a prominent feature at lambda 6708 A. Its identification with Li I remains controversial due to a poor knowledge of the spectra of rare-earth elements that are strongly enhanced in peculiar stars and can potentially provide an alternative identification. We suggest to investigate the 6708 line in Ap stars with strong magnetic fields. In these objects the magnetic broadening and splitting provides an additional powerful criterium for line identification, allowing to use the whole line profile instead of a mere coincidence of the observed and predicted wavelength. Due to a small separation of the Li I doublet components, their magnetic splitting pattern deviates from the one expected for the Zeeman effect even in relatively weak fields. We carry out detailed calculations of the transition between the Zeeman and Paschen-Back regimes in the magnetic splitting of the Li I line and compute polarized synthetic spectra for the range of field strength expected in Ap stars. Theor...

  17. Transportation characteristics of nolatrexed in three tumor cell lines

    Institute of Scientific and Technical Information of China (English)

    LI Yi-lei; ZHAO Ai-guo; WU Shu-guang

    2002-01-01

    Objective:To investigate the association of the transportation characteristics of nolatrexed in tumor cells with its drug sensitivity. Methods: The sensitivity of 3 tumor cell lines, C6, SRS82 and LoVo, to nolatrexed were determined by growth inhibition study. After exposure to 20 μmol/L nolatrexed at different time intervals ranging from 0 to 30 min, or to nolatrexed at different concentrations ranging from 0 to 40μmol/L for 10 min, the intracellular drug concentration was measured using high-performance liquid chromatography. Results: C6 was the most sensitive cell line among the three, with sensitivity 6. 8-fold and 13.8-fold those of SRS-82 and LoVo cells respectively. Transportation of nolatrexed in the 3 cell lines were qualitatively similar, which rapidly achieved steady-state within 5 min, and linear relationship between the intracellular and extracellular drug concentration was observed. The intracellular steady-state level achieved in C6 was significantly higher than those in the other two cell lines, the latter having comparable levels. Conclusion: Nolatrexed enters the cell very quickly and different transport capacities are involved in the generation of varied sensitivity to nolatrexed in tumor cells.

  18. Electronic transport of bilayer graphene with asymmetry line defects

    Science.gov (United States)

    Zhao, Xiao-Ming; Wu, Ya-Jie; Chen, Chan; Liang, Ying; Kou, Su-Peng

    2016-11-01

    In this paper, we study the quantum properties of a bilayer graphene with (asymmetry) line defects. The localized states are found around the line defects. Thus, the line defects on one certain layer of the bilayer graphene can lead to an electric transport channel. By adding a bias potential along the direction of the line defects, we calculate the electric conductivity of bilayer graphene with line defects using the Landauer-Büttiker theory, and show that the channel affects the electric conductivity remarkably by comparing the results with those in a perfect bilayer graphene. This one-dimensional line electric channel has the potential to be applied in nanotechnology engineering. Project supported by the National Basic Research Program of China (Grant Nos. 2011CB921803 and 2012CB921704), the National Natural Science Foundation of China (Grant Nos. 11174035, 11474025, 11504285, and 11404090), the Specialized Research Fund for the Doctoral Program of Higher Education, China, the Fundamental Research Funds for the Central Universities, China, the Scientific Research Program Fund of the Shaanxi Provincial Education Department, China (Grant No. 15JK1363), and the Young Talent Fund of University Association for Science and Technology in Shaanxi Province, China.

  19. SPES-2, AP600 intergral system test S01007 2 inch CL to core make-up tank pressure balance line break

    Energy Technology Data Exchange (ETDEWEB)

    Bacchiani, M.; Medich, C.; Rigamonti, M. [SIET S.p.A. Piacenza (Italy)] [and others

    1995-09-01

    The SPES-2 is a full height, full pressure experimental test facility reproducing the Westinghouse AP600 reactor with a scaling factor of 1/395. The experimental plant, designed and operated by SIET in Piacenza, consists of a full simulation of the AP600 primary core cooling system including all the passive and active safety systems. In 1992, Westinghouse, in cooperation with ENEL (Ente Nazionale per l` Energia Elettrica), ENEA (Enter per le numove Technlogie, l` Energia e l` Ambient), Siet (Societa Informazioni Esperienze Termoidraulich) and ANSALDO developed an experimental program to test the integrated behaviour of the AP600 passive safety systems. The SPES-2 test matrix, concluded in November 1994, has examined the AP600 passive safety system response for a range of small break LOCAs at different locations on the primary system and on the passive system lines; single steam generator tube ruptures with passive and active safety systems and a main steam line break transient to demonstrate the boration capability of passive safety systems for rapid cooldown. Each of the tests has provided detailed experimental results for verification of the capability of the analysis methods to predict the integrated passive safety system behaviour. Cold and hot shakedown tests have been performed on the facility to check the characteristics of the plant before starting the experimental campaign. The paper first presents a description of the SPES-2 test facility then the main results of S01007 test {open_quotes}2{close_quotes} Cold Leg (CL) to Core Make-up Tank (CMT) pressure balance line break{close_quotes} are reported and compared with predictions performed using RELAP5/mod3/80 obtained by ANSALDO through agreement with U.S.N.R.C. (U.S. Nuclear Regulatory Commission). The SPES-2 nodalization and all the calculations here presented were performed by ANSALDO and sponsored by ENEL as a part of pre-test predictions for SPES-2.

  20. Bending magnets for the CBA beam-transport line

    Energy Technology Data Exchange (ETDEWEB)

    Thern, R.E.

    1983-01-01

    The beam-transport line from the AGS to CBA requires 68 large bending magnets, consisting of pure dipoles and two types of combined function gradient magnets. All three types were designed with magnetic-field calculation program POISSON, using the same exterior dimensions and coil package. The design goal of +-1% momentum acceptance for the transport line required a wide horizontal aperture, with a much-smaller vertical aperture for economy. Two prototypes of one gradient magnet were built, and a facility constructed to measure them and the later production magnets. Measurements were done using both a long coil and a point coil (Rawson-Lush gaussmeter). Preliminary results show ..delta..B/B < 0.2 x 10/sup -3/, ..delta..G/G < 0.3 x 10/sup -2/, and ..delta..B/sub 2//B < 0.3 x 10/sup -4/ cm/sup -2/ over the beam aperture. Due to end effects, the actual gradient differs from the design gradient by 1%, which has been compensated for in the beam-line design.

  1. Phenomena identification and ranking tables for Westinghouse AP600 small break loss-of-coolant accident, main steam line break, and steam generator tube rupture scenarios

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, G.E.; Fletcher, C.D.; Davis, C.B. [and others

    1997-06-01

    This report revision incorporates new experimental evidence regarding AP600 behavior during small break loss-of-coolant accidents. This report documents the results of Phenomena Identification and Ranking Table (PIRT) efforts for the Westinghouse AP600 reactor. The purpose of this PIRT is to identify important phenomena so that they may be addressed in both the experimental programs and the RELAP5/MOD3 systems analysis computer code. In Revision of this report, the responses of AP600 during small break loss-of-coolant accident, main steam line break, and steam generator tube rupture accident scenarios were evaluated by a committee of thermal-hydraulic experts. Committee membership included Idaho National Engineering and Environmental Laboratory staff and recognized thermal-hydraulic experts from outside of the laboratory. Each of the accident scenarios was subdivided into separate, sequential periods or phases. Within each phase, the plant behavior is controlled by, at most, a few thermal-hydraulic processes. The committee identified the phenomena influencing those processes, and ranked & influences as being of high, medium, low, or insignificant importance. The primary product of this effort is a series of tables, one for each phase of each accident scenario, describing the thermal-hydraulic phenomena judged by the committee to be important, and the relative ranking of that importance. The rationales for the phenomena selected and their rankings are provided. This document issue incorporates an update of the small break loss-of-coolant accident portion of the report. This revision is the result of the release of experimental evidence from AP600-related integral test facilities (ROSA/AP600, OSU, and SPES) and thermal-hydraulic expert review. The activities associated with this update were performed during the period from June 1995 through November 1996. 8 refs., 26 figs., 42 tabs.

  2. A quantum transport model for atomic line radiation in plasmas*

    Science.gov (United States)

    Rosato, Joël

    2017-02-01

    Emission and absorption lines in plasmas are investigated theoretically using a phase space formulation of quantum electrodynamics. A transport equation for the one-photon Wigner function is derived and formulated in terms of the noncommutative Moyal product. This equation reduces to the standard radiative transfer equation at the large spectral band limit, when the characteristic spectral band of the emission and absorption coefficients is larger than the inverse photon absorption length and time. We examine deviations to this limit. An ideal slab geometry is considered. The Wigner function relative to hydrogen Lyman α in stellar atmospheric conditions is calculated.

  3. New magnet transport system for the LHC beam transfer lines

    CERN Multimedia

    Patrice Loïez

    2003-01-01

    The first of 700 magnets has been installed in one of the two transfer tunnels built to transfer the SPS beam into the LHC. The start of this first installation phase of the LHC transfer lines provides the opportunity to launch a new and highly original modular system for transporting and installing all kinds of magnets in very narrow tunnels. The system (pictured here in one of the tunnels) is based on very compact bogies, up to four of which can be coupled together to form a convoy. The wheels are fitted with individual motors enabling them to swivel through an angle of 90° and the convoy to move laterally. The lead vehicle is powered by an electric rail set into the roof of the tunnel. The system is backed up by electrical batteries that enable it to operate in the absence of an outside power source or in the event of power failure. Last but not least, for the long-distance transport of magnets, it can be optically guided by a line traced on the tunnel floor. The convoy moves through the particularly narr...

  4. The new control system of HLS linac and transport line

    Institute of Scientific and Technical Information of China (English)

    LIU Gong-Fa; LI Wei-Min; LI Jing-Yi; LI Chuan; CHEN Li-Ping; BAO Xun; WANG Ji-Gang; XUAN Ke

    2005-01-01

    The new linac and transport line control system of Hefei Light Source (HLS) is a distributed control system based on EPICS (Experimental Physics and Industrial Control System). Industrial PC (IPC) is widely used as not only Input/Output Controller (IOC) but also device controller. Besides industrial PC, PLC and microcontroller are also used as device controllers. The software for industrial PC based device controller is developed based on VxWorks real-time operating system. The software for PLC and microcontroller are written with ladder software package and assemble language, respectively. PC with Linux and SUN workstation with Solaris are used as operator interfaces (OPI). High level control is made up of some EPICS tools and Tcl/Tk scripts.

  5. Observation and analysis of self-amplified spontaneous emission at the APS low-energy undulator test line

    CERN Document Server

    Arnold, N D; Banks, G; Bechtold, R; Beczek, K; Benson, C; Berg, S; Berg, W; Biedron, S G; Biggs, J A; Boerste, K; Borland, M; Bosek, M; Brzowski, W R; Budz, J; Carwardine, J A; Castro, P; Chae, Y C; Christensen, S; Clark, C; Conde, M; Crosbie, E A; Decker, G A; Dejus, Roger J; Deleon, H; Den Hartog, P K; Deriy, B N; Dohan, D; Dombrowski, P; Donkers, D; Doose, C L; Dortwegt, R J; Edwards, G A; Eidelman, Y; Erdmann, M J; Error, J J; Ferry, R; Flood, R; Forrestal, J; Freund, H; Friedsam, H; Gagliano, J; Gai, W; Galayda, J N; Gerig, R; Gilmore, R L; Gluskin, E; Goeppner, G A; Goetzen, J; Gold, C; Grelick, A E; Hahne, M W; Hanuska, S; Harkay, K C; Harris, G; Hillman, A L; Hogrefe, R; Hoyt, J; Huang, Z; Jagger, J M; Jansma, W G; Jaski, M; Jones, S J; Keane, R T; Kelly, A L; Keyser, C; Kim, K J; Kim, S H; Kirshenbaum, M; Klick, J H; Knoerzer, K; Knott, M; Koldenhoven, R J; Labuda, S; Laird, R; Lang, J; Lenkszus, F R; Lessner, E S; Lewellen, J W; Li, Y; Lill, R M; Lumpkin, Alex H; Makarov, O A; Markovich, G M; McDowell, M; McDowell, W P; McNamara, P E; Meier, T; Meyer, D; Michalek, W; Milton, S V; Moe, H; Moog, E; Morrison, L; Nassiri, A; Noonan, J R; Otto, R; Pace, J; Pasky, S J; Penicka, J M; Pietryla, A F; Pile, G; Pitts, C; Power, J; Powers, T; Putnam, C C; Puttkammer, A J; Reigle, D; Reigle, L; Ronzhin, D; Rotela, E R; Russell, E F; Sajaev, Vadim; Sarkar, S; Scapino, J C; Schröder, K; Seglem, R A; Sereno, N S; Sharma, S K; Sidarous, J F; Singh, O; Smith, T L; Soliday, R; Sprau, G A; Stein, S J; Stejskal, B; Svirtun, V; Teng, L C; Theres, E; Thompson, K; Tieman, B J; Torres, J A; Trakhtenberg, E; Travish, G; Trento, G F; Vacca, J; Vasserman, I B; Vinokurov, N A; Walters, D R; Wang, J; Wang, X J; Warren, J; Wesling, S; Weyer, D L; Wiemerslage, G; Wilhelmi, K; Wright, R; Wyncott, D; Xu, S; Yang, B X; Yoder, W; Zabel, R B

    2001-01-01

    Exponential growth of self-amplified spontaneous emission at 530 nm was first experimentally observed at the Advanced Photon Source low-energy undulator test line in December 1999. Since then, further detailed measurements and analysis of the results have been made. Here, we present the measurements and compare these with calculations based on measured electron beam properties and theoretical expectations.

  6. The Broad-Lined Type Ic SN 2012ap and the Nature of Relativistic Supernovae Lacking a Gamma-ray Burst Detection

    CERN Document Server

    Milisavljevic, D; Parrent, J T; Soderberg, A M; Fesen, R A; Mazzali, P; Maeda, K; Sanders, N E; Cenko, S B; Silverman, J M; Filippenko, A V; Kamble, A; Chakraborti, S; Drout, M R; Kirshner, R P; Pickering, T E; Kawabata, K; Hattori, T; Hsiao, E Y; Stritzinger, M D; Marion, G H; Vinko, J; Wheeler, J C

    2014-01-01

    We present ultraviolet, optical, and near-infrared observations of SN 2012ap, a broad-lined Type Ic supernova in the galaxy NGC 1729 that produced a relativistic and rapidly decelerating outflow without a gamma-ray burst signature. Photometry and spectroscopy follow the flux evolution from -13 to +272 days past the B-band maximum of -17.4 +/- 0.5 mag. The spectra are dominated by Fe II, O I, and Ca II absorption lines at ejecta velocities of 20,000 km/s that change slowly over time. Other spectral absorption lines are consistent with contributions from photospheric He I, and hydrogen may also be present at higher velocities (> 27,000 km/s). We use these observations to estimate explosion properties and derive a total ejecta mass of 2.7 Msolar, a kinetic energy of 1.0x10^{52} erg, and a 56Ni mass of 0.1-0.2 Msolar. Nebular spectra (t > 200d) exhibit an asymmetric double-peaked [OI] 6300,6364 emission profile that we associate with absorption in the supernova interior, although toroidal ejecta geometry is an al...

  7. The Broad-Lined Type Ic SN 2012ap and the Nature of Relativistic Supernovae Lacking a Gamma-Ray Burst Detection

    Science.gov (United States)

    Milisavljevic, D.; Margutti, R.; Parrent, J. T.; Soderberg, A. M.; Fesen, R. A.; Mazzali, P.; Maeda, K.; Sanders, N. E.; Cenko, S. B.; Silverman, J. M.

    2014-01-01

    We present ultraviolet, optical, and near-infrared observations of SN2012ap, a broad-lined Type Ic supernova in the galaxy NGC 1729 that produced a relativistic and rapidly decelerating outflow without a gamma-ray burst signature. Photometry and spectroscopy follow the flux evolution from -13 to +272 days past the B-band maximum of -17.4 +/- 0.5 mag. The spectra are dominated by Fe II, O I, and Ca II absorption lines at ejecta velocities of v approx. 20,000 km s(exp. -1) that change slowly over time. Other spectral absorption lines are consistent with contributions from photospheric He I, and hydrogen may also be present at higher velocities (v approx. greater than 27,000 km s(exp. -1)). We use these observations to estimate explosion properties and derive a total ejecta mass of 2.7 Solar mass, a kinetic energy of 1.0×1052 erg, and a (56)Ni mass of 0.1-0.2 Solar mass. Nebular spectra (t > 200 d) exhibit an asymmetric double-peaked [O I] lambda lambda 6300, 6364 emission profile that we associate with absorption in the supernova interior, although toroidal ejecta geometry is an alternative explanation. SN2012ap joins SN2009bb as another exceptional supernova that shows evidence for a central engine (e.g., black-hole accretion or magnetar) capable of launching a non-negligible portion of ejecta to relativistic velocities without a coincident gamma-ray burst detection. Defining attributes of their progenitor systems may be related to notable properties including above-average environmental metallicities of Z approx. greater than Solar Z, moderate to high levels of host-galaxy extinction (E(B -V ) > 0.4 mag), detection of high-velocity helium at early epochs, and a high relative flux ratio of [Ca II]/[O I] > 1 at nebular epochs. These events support the notion that jet activity at various energy scales may be present in a wide range of supernovae.

  8. THE BROAD-LINED Type Ic SN 2012ap AND THE NATURE OF RELATIVISTIC SUPERNOVAE LACKING A GAMMA-RAY BURST DETECTION

    Energy Technology Data Exchange (ETDEWEB)

    Milisavljevic, D.; Margutti, R.; Parrent, J. T.; Soderberg, A. M.; Sanders, N. E.; Kamble, A.; Chakraborti, S.; Drout, M. R.; Kirshner, R. P. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Fesen, R. A. [Department of Physics and Astronomy, Dartmouth College, 6127 Wilder Laboratory, Hanover, NH 03755 (United States); Mazzali, P. [Astrophysics Research Institute, Liverpool John Moores University, Liverpool L3 5RF (United Kingdom); Maeda, K. [Department of Astronomy, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-8502 (Japan); Cenko, S. B. [Astrophysics Science Division, NASA Goddard Space Flight Center, Mail Code 661, Greenbelt, MD 20771 (United States); Silverman, J. M. [University of Texas at Austin, 1 University Station C1400, Austin, TX 78712-0259 (United States); Filippenko, A. V. [Department of Astronomy, University of California, Berkeley, CA 94720-3411 (United States); Pickering, T. E. [Southern African Large Telescope, P.O. Box 9, Observatory 7935, Cape Town (South Africa); Kawabata, K. [Hiroshima Astrophysical Science Center, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526 (Japan); Hattori, T. [Subaru Telescope, National Astronomical Observatory of Japan, Hilo, HI 96720 (United States); Hsiao, E. Y. [Carnegie Observatories, Las Campanas Observatory, Colina El Pino, Casilla 601 (Chile); Stritzinger, M. D., E-mail: dmilisav@cfa.harvard.edu [Department of Physics and Astronomy, Aarhus University, Ny Munkegade, DK-8000 Aarhus C (Denmark); and others

    2015-01-20

    We present ultraviolet, optical, and near-infrared observations of SN 2012ap, a broad-lined Type Ic supernova in the galaxy NGC 1729 that produced a relativistic and rapidly decelerating outflow without a gamma-ray burst signature. Photometry and spectroscopy follow the flux evolution from –13 to +272 days past the B-band maximum of –17.4 ± 0.5 mag. The spectra are dominated by Fe II, O I, and Ca II absorption lines at ejecta velocities of v ≈ 20,000 km s{sup –1} that change slowly over time. Other spectral absorption lines are consistent with contributions from photospheric He I, and hydrogen may also be present at higher velocities (v ≳ 27,000 km s{sup –1}). We use these observations to estimate explosion properties and derive a total ejecta mass of ∼2.7 M {sub ☉}, a kinetic energy of ∼1.0 × 10{sup 52} erg, and a {sup 56}Ni mass of 0.1-0.2 M {sub ☉}. Nebular spectra (t > 200 days) exhibit an asymmetric double-peaked [O I] λλ6300, 6364 emission profile that we associate with absorption in the supernova interior, although toroidal ejecta geometry is an alternative explanation. SN 2012ap joins SN 2009bb as another exceptional supernova that shows evidence for a central engine (e.g., black hole accretion or magnetar) capable of launching a non-negligible portion of ejecta to relativistic velocities without a coincident gamma-ray burst detection. Defining attributes of their progenitor systems may be related to notable observed properties including environmental metallicities of Z ≳ Z {sub ☉}, moderate to high levels of host galaxy extinction (E(B – V) > 0.4 mag), detection of high-velocity helium at early epochs, and a high relative flux ratio of [Ca II]/[O I] >1 at nebular epochs. These events support the notion that jet activity at various energy scales may be present in a wide range of supernovae.

  9. Error analysis in post linac to driver linac transport beam line of RAON

    Science.gov (United States)

    Kim, Chanmi; Kim, Eun-San

    2016-07-01

    We investigated the effects of magnet errors in the beam transport line connecting the post linac to the driver linac (P2DT) in the Rare Isotope Accelerator in Korea (RAON). The P2DT beam line is bent by 180-degree to send the radioactive Isotope Separation On-line (ISOL) beams accelerated in Linac-3 to Linac-2. This beam line transports beams with multi-charge state 132Sn45,46,47. The P2DT beam line includes 42 quadrupole, 4 dipole and 10 sextupole magnets. We evaluate the effects of errors on the trajectory of the beam by using the TRACK code, which includes the translational and the rotational errors of the quadrupole, dipole and sextupole magnets in the beam line. The purpose of this error analysis is to reduce the rate of beam loss in the P2DT beam line. The distorted beam trajectories can be corrected by using six correctors and seven monitors.

  10. Control system of HLS transport line and Linac focusing power supplies

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The control system of transport line and Linac focusing power supplies of Hefei Light Source was built upon Experimental Physics and Industrial Control System. The hardware construction, software design and performance test of the control system are described.

  11. Resolving magnetic field line stochasticity and parallel thermal transport in MHD simulations

    Energy Technology Data Exchange (ETDEWEB)

    Nishimura, Y.; Callen, J.D.; Hegna, C.C. [Univ. of Wisconsin, Madison, WI (United States). Dept. of Engineering Physics

    1998-12-31

    Heat transport along braided, or chaotic magnetic field lines is a key to understand the disruptive phase of tokamak operations, both the major disruption and the internal disruption (sawtooth oscillation). Recent sawtooth experimental results in the Tokamak Fusion Test Reactor (TFTR) have inferred that magnetic field line stochasticity in the vicinity of the q = 1 inversion radius plays an important role in rapid changes in the magnetic field structures and resultant thermal transport. In this study, the characteristic Lyapunov exponents and spatial correlation of field line behaviors are calculated to extract the characteristic scale length of the microscopic magnetic field structure (which is important for net radial global transport). These statistical values are used to model the effect of finite thermal transport along magnetic field lines in a physically consistent manner.

  12. Transportation Lines on the Atlantic, Gulf, and Pacific Coasts, Transportation Series 5, 1980.

    Science.gov (United States)

    1982-05-01

    1962STEEL EXXON BAY STATE ITUG. DIESEL. 105 1043 1097? 31 311 14B 148 2800 . 60, A0S 0 EvEREO. 1970~ ’S8L EXXON CRYSTAL RIVER 00 91 960 060 270 270 1...TOWING CO. I TOWING$, LOGS, AND FREIGHT PUSS? SOUND ANB ALASKA 7 1supoNTy DENEMUReS. E. I., a go. 3 BULK LIDUID EWEMICALS ER1LAWARE RIVER, ENEsAP1AKE

  13. Field-line transport in stochastic magnetic fields: Percolation, Levy flights, and non-Gaussian dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Zimbardo, G.; Veltri, P. (Dipartimento di Fisica, Universita della Calabria, I-87030 Arcavacata di Rende (Italy))

    1995-02-01

    The transport of magnetic field lines is studied numerically in the case where strong three-dimensional magnetic fluctuations are superimposed to a uniform average magnetic field. The magnetic percolation of field lines between magnetic islands is found, as well as a non-Gaussian regime where the field lines exhibit Levy random walks, changing from Levy flights to trapped motion. Anomalous diffusion laws [l angle][Delta][ital x][sub [ital i

  14. Cost Optimization for Series-Parallel Petroleum Transportation Pape-Lines under Reliability Constraints

    Directory of Open Access Journals (Sweden)

    M. Amara,

    2014-01-01

    Full Text Available This paper uses an ant colony meta-heuristic optimization method to solve the cost-optimization problem in petrolum industry. This problem is known as total investment-cost minimization of series-parallel transportation pape lines. Redundant Electro-Pumpe coupled to the papes lines are included to achieve a desired level of availability. System availability is represented by a multi-state availability function. The Electro-pumpe (pape-lines are characterized by their capacity, availability and cost. These electro-pumpes are chosen among a list of products available on the market. The proposed meta-heuristic seeks to find the best minimal cost of petrol transportation system configuration with desired availability. To estimate the series-parallel pape lines availability, a fast method based on universal moment generating function (UMGF is suggested. The ant colony approach is used as an optimization technique. An example of petrol transportation system is presented.

  15. The IFUSP microtron accelerator beam transport line; Linha de transporte de feixe do acelerador microtron do IFUSP

    Energy Technology Data Exchange (ETDEWEB)

    Rios, Paulo Beolchi

    2002-07-01

    In this work, the electron optical project of the IFUSP microtron beam transport line is presented, including the operational values for the parameters of the dipolar and quadrupolar electromagnets, as well as their location along the beam line. Analytical calculations and computer simulations were performed to obtain these results, and a programming tool was developed in order to analyze the beam parameters and to help studying racetrack microtrons. The electron optical simulations were split into two different study cases: the microtron booster, and the transfer line. In the first case, it was determined the main operational parameters of a microtron working far from its usual stability conditions. In the latter, it was done the basic design of the linking line between the booster and main (not yet built) microtrons, and between them and the experimental hall, with a total path length of approximately 32 m including large horizontal and vertical deflections with variable beam energy. (author)

  16. Spin helical states and spin transport of the line defect in silicene lattice

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Mou; Chen, Dong-Hai; Wang, Rui-Qiang [Laboratory of Quantum Engineering and Quantum Materials, School of Physics and Telecommunication Engineering, South China Normal University, Guangzhou 510006 (China); Bai, Yan-Kui, E-mail: ykbai@semi.ac.cn [College of Physical Science and Information Engineering and Hebei Advance Thin Films Laboratory, Hebei Normal University, Shijiazhuang, Hebei 050024 (China)

    2015-02-06

    We investigated the electronic structure of a silicene-like lattice with a line defect under the consideration of spin–orbit coupling. In the bulk energy gap, there are defect related bands corresponding to spin helical states localized beside the defect line: spin-up electrons flow forward on one side near the line defect and move backward on the other side, and vice versa for spin-down electrons. When the system is subjected to random distribution of spin-flipping scatterers, electrons suffer much less spin-flipped scattering when they transport along the line defect than in the bulk. An electric gate above the line defect can tune the spin-flipped transmission, which makes the line defect as a spin-controllable waveguide. - Highlights: • Band structure of silicene with a line defect. • Spin helical states around the line defect and their probability distribution features. • Spin transport along the line defect and that in the bulk silicene.

  17. TRUNK WITH BRANCHES PUBLIC TRANSPORT LINE SCHEDULLING UNDER CONDITION OF UNIFORM HEADWAY OPERATION

    Directory of Open Access Journals (Sweden)

    Nikola Krstanoski

    2015-12-01

    Full Text Available Planning of public transport network for good spatial coverage may lead to an operation of radial or diametrical public transport lines that branch at one or both ends of the line. This trunk with branches operation means that all vehicles on the line serve the main - trunk section, usually covering the central area of the city, and then split in two or more branches in the peripheral areas, in order to offer better spatial service. The scheduling of this type of operation is not an easy task since only certain patterns of headways and therefore passenger demands can be met, if efficient operation under the condition of uniform headways on all sections on the line is required. In this article the author analyzes the key variables that define the uniform headway operation and establishes their relationships, thus providing answer to the question what headway patterns are feasible and if they could be acceptable for real life operation.

  18. Design of the beam transport line and injection system of the compact storage ring for TTX

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    In this article, we present the design of the beam transport line and injection system of the compact storage ring for the Tsinghua Thomson scattering X-ray (TTX) source. The layout of the beam transport line fits in with the limited available space. The injection system is simplified, consisting of only one single kicker; the stray field on the reference orbit is also reduced without the septum magnet. We choose a travelling wave kicker and present both 2D and 3D simulations for the structure design.

  19. Enhancing the accelerated beam current in the booster synchrotron by optimizing the transport line beam propagation

    Indian Academy of Sciences (India)

    Saini R S; Tyagi Y; Ghodke A D; Puntambekar T A

    2016-04-01

    In this paper, we present the results of transverse beam emittance and twiss parameter measurement of an electron beam, delivered by a 20 MeV microtron which is used as a pre-injector system for a booster synchrotron in the Indus Accelerator Facility at RRCAT Indore. Based on these measured beam parameters, beam optics of a transport line was optimized and its results are alsodiscussed in this paper. This beam transport line is used to transport the electron beam from the 20MeV microtron to the booster synchrotron. The booster synchrotron works as a main injector for Indus-1 and Indus-2 synchrotron radiation facilities. To optimize the beam optics of a transport linefor proper beam transmission through the line as well as to match the beam twiss parameters at the beam injection point of another accelerator, it is necessary to know the transverse beam emittance and twiss parameters of the beam coming from the first one. A MATLAB-based GUI program has been developed to calculate the beam emittance and twiss parameters, using quadrupole scanmethod. The measured parameters have been used for beam transport line optimization and twiss parameters matching at booster injection point. After this optimization, an enhancement of ∼50% beam current has been observed in the booster synchrotron.

  20. APS Science 2007.

    Energy Technology Data Exchange (ETDEWEB)

    2008-05-30

    This report provides research highlights from the Advanced Photon Source (APS). Although these highlights represent less than 10% of the published work from the APS in 2007, they give a flavor of the diversity and impact of user research at the facility. In the strategic planning the aim is to foster the growth of existing user communities and foresee new areas of research. This coming year finds the APS engaged in putting together, along with the users, a blueprint for the next five years, and making the case for a set of prioritized investments in beamlines, the accelerator, and infrastructure, each of which will be transformational in terms of scientific impact. As this is written plans are being formulated for an important user workshop on October 20-21, 2008, to prioritize strategic plans. The fruit from past investments can be seen in this report. Examples include the creation of a dedicated beamline for x-ray photon correlation spectroscopy at Sector 8, the evolution of dedicated high-energy x-ray scattering beamlines at sectors 1 and 11, a dedicated imaging beamline at Sector 32, and new beamlines for inelastic scattering and powder diffraction. A single-pulse facility has been built in collaboration with Sector 14 (BioCARS) and Phil Anfinrud at the National Institutes of Health, which will offer exceptionally high flux for single-pulse diffraction. The nanoprobe at Sector 26, built and operated jointly by the Argonne Center for Nanoscale Materials and the X-ray Operations and Research (XOR) section of the APS X-ray Science Division, has come on line to define the state of the art in nanoscience.

  1. Choline transport via choline transporter-like protein 1 in conditionally immortalized rat syncytiotrophoblast cell lines TR-TBT.

    Science.gov (United States)

    Lee, N-Y; Choi, H-M; Kang, Y-S

    2009-04-01

    Choline is an essential nutrient for phospholipids and acetylcholine biosynthesis in normal development of fetus. In the present study, we investigated the functional characteristics of choline transport system and inhibitory effect of cationic drugs on choline transport in rat conditionally immortalized syncytiotrophoblast cell line (TR-TBT). Choline transport was weakly Na(+) dependent and significantly influenced by extracellular pH and by membrane depolarization. The transport process of choline is saturable with Michaelis-Menten constants (K(m)) of 68microM and 130microM in TR-TBT 18d-1 and TR-TBT 18d-2 respectively. Choline uptake in the cells was inhibited by unlabeled choline and hemicholinium-3 as well as various organic cations including guanidine, amiloride and acetylcholine. However, the prototypical organic cation tetraethylammonium and cimetidine showed very little inhibitory effect of choline uptake in TR-TBT cells. RT-PCR revealed that choline transporter-like protein 1 (CTL1) and organic cation transporter 2 (OCT2) are expressed in TR-TBT cells. The transport properties of choline in TR-TBT cells were similar or identical to that of CTL1 but not OCT2. CTL1 was also detected in human placenta. In addition, several cationic drugs such as diphenhydramine and verapamil competitively inhibited choline uptake in TR-TBT 18d-1 with K(i) of 115microM and 55microM, respectively. Our results suggest that choline transport system, which has intermediate affinity and weakly Na(+) dependent, in TR-TBT seems to occur through a CTL1 and this system may have relevance with the uptake of pharmacologically important organic cation drugs.

  2. 76 FR 52731 - On-Line Complaint Form for Service-Related Issues in Air Transportation

    Science.gov (United States)

    2011-08-23

    ... Office of the Secretary On-Line Complaint Form for Service-Related Issues in Air Transportation AGENCY... is complaining, flight date, flight number, and origin and destination cities of complainant's trip...- travel related information from the ACPD. The Department has limited its informational request to...

  3. 8 CFR 241.24 - Notice to transportation line of alien's exclusion.

    Science.gov (United States)

    2010-01-01

    ... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Notice to transportation line of alien's exclusion. 241.24 Section 241.24 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS APPREHENSION AND DETENTION OF ALIENS ORDERED REMOVED Deportation of Excluded Aliens (for...

  4. 8 CFR 241.9 - Notice to transportation line of alien's removal.

    Science.gov (United States)

    2010-01-01

    ... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Notice to transportation line of alien's removal. 241.9 Section 241.9 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS APPREHENSION AND DETENTION OF ALIENS ORDERED REMOVED Post-hearing Detention and Removal §...

  5. Cells in G2/M phase increased in human nasopharyngeal carcinoma cell line by EBV-LMP1 through activation of NF-κB and AP-1

    Institute of Scientific and Technical Information of China (English)

    LIN DENG; JING YANG; XIAO RONG ZHAO; XI YUN DENG; LIANG ZENG; HUAN HUA GU; MIN TANG; YA CAO

    2003-01-01

    Although previous studies showed that the principal oncoprotein encoded by Epstein-Barr virus, latentmembrane protein 1(LMP1), could induce the nasopharyngeal carcinoma cells in G2/M phase increased, littleis known about the target molecules and mechanisms. The present study demonstrated that LMP1 couldinduce the accumulation of p53 protein and upregulate its transactivity in a dose dependent manner, whichresulted in the decrease of the kinase activity of cdc2/cyclin B complex and inducing arrest at G2/M phasethrough the activation of NF-κB and AP-1 signaling pathways, and the effect of NF-κB was more obviousthan that of AP-1. This study provided some significant evidence for further elucidating the molecularmechanisms that LMP1 had effects on the surveillance mechanism of cell cycle and promoting the survivalof transformed cells and tumorigenesis.

  6. Transport and dosimetric solutions for the ELIMED laser-driven beam line

    Energy Technology Data Exchange (ETDEWEB)

    Cirrone, G.A.P. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Romano, F. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Medical Physics School, University of Catania, Via S. Sofia 64 - 95125 Catania (Italy); Scuderi, V. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Institute of Physics ASCR, v.v.i. (FZU), ELI-Beamlines Project, Na Slovance 2, 182 21 Prague (Czech Republic); Amato, A. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Candiano, G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Medical Physics School, University of Catania, Via S. Sofia 64 - 95125 Catania (Italy); Cuttone, G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Giove, D. [INFN Sezione di Milano, Via Celoria 16, Milano (Italy); Korn, G.; Krasa, J. [Institute of Physics ASCR, v.v.i. (FZU), ELI-Beamlines Project, Na Slovance 2, 182 21 Prague (Czech Republic); Leanza, R. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Universitá degli Studi di Catania, Dipartimento di Fisica e Astronomia, Via S. Sofia 64, Catania (Italy); Manna, R. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Maggiore, M. [INFN-LNL, Viale dell' Universitá 2 - 35020 Legnaro (PD) (Italy); Marchese, V. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Margarone, D. [Institute of Physics ASCR, v.v.i. (FZU), ELI-Beamlines Project, Na Slovance 2, 182 21 Prague (Czech Republic); Milluzzo, G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Universitá degli Studi di Catania, Dipartimento di Fisica e Astronomia, Via S. Sofia 64, Catania (Italy); Petringa, G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Sabini, M.G. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Azienda Ospedaliera Cannizzaro, Via Messina 829 - 95100 Catania (Italy); Schillaci, F. [INFN-LNS, Via S. Sofia 62 - 95125 Catania (Italy); Medical Physics School, University of Catania, Via S. Sofia 64 - 95125 Catania (Italy); and others

    2015-10-01

    Within 2017, the ELIMED (ELI-Beamlines MEDical applications) transport beam-line and dosimetric systems for laser-generated beams will be installed at the ELI-Beamlines facility in Prague (CZ), inside the ELIMAIA (ELI Multidisciplinary Applications of laser–Ion Acceleration) interaction room. The beam-line will be composed of two sections: one in vacuum, devoted to the collecting, focusing and energy selection of the primary beam and the second in air, where the ELIMED beam-line dosimetric devices will be located. This paper briefly describes the transport solutions that will be adopted together with the main dosimetric approaches. In particular, the description of an innovative Faraday Cup detector with its preliminary experimental tests will be reported.

  7. Reversal of resistance by GF120918 in cell lines expressing the ABC half-transporter, MXR

    DEFF Research Database (Denmark)

    de Bruin, M; Miyake, K; Litman, Thomas;

    1999-01-01

    assays, rhodamine and calcein efflux assays, and confocal microscopy in cell lines expressing different multidrug resistance transporters. At a concentration of 1 microM in cytotoxicity assays, GF120918 was able to sensitize both S1-B1-20, a subline expressing P-glycoprotein (Pgp), and S1-M1......The emergence of several newly identified members of the ABC transporter family has necessitated the development of antagonists that are able to inhibit more than one transporter. We assessed the ability of the chemosensitizer GF120918 to function as a multispecific antagonist using cytotoxicity......-80, a subline expressing a newly identified mitoxantrone transporter, MXR. GF120918 was ineffective in sensitizing MRP-overexpressing MCF-7 VP-16 cells to etoposide as determined by cytotoxicity studies. In flow cytometry experiments, rhodamine 123 efflux in S1-B1-20 cells was decreased at GF120918...

  8. Railroad Lines - RAILROAD_100K_NTAD_IN: Railroad Network in Indiana (National Transportation Atlas Database, 1:100,000, Line Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — RAILROAD_NTAD_100K_SW is a 1:100,000 scale line shapefile which is a subset of the rail network. Bureau of Transportation Statistics metadata states - "The Rail...

  9. Ambipolar transport via trapped-electron whistler instability along open magnetic field lines.

    Science.gov (United States)

    Guo, Zehua; Tang, Xian-Zhu

    2012-09-28

    An open field line plasma is bounded by a chamber wall which intercepts the magnetic field. Steady state requires an upstream plasma source balancing the particle loss to the boundary. In cases where the electrons have a long mean free path, ambipolarity in parallel transport critically depends on collisionless detrapping of the electrons via wave-particle interaction. The trapped-electron whistler instability, whose nonlinear saturation produces a spectrum of whistler waves that is responsible for the electron detrapping flux, is shown to be an unusually robust kinetic instability, which is essential to the universality of the ambipolar constraint in plasma transport.

  10. Extracting potential bus lines of Customized City Bus Service based on public transport big data

    Science.gov (United States)

    Ren, Yibin; Chen, Ge; Han, Yong; Zheng, Huangcheng

    2016-11-01

    Customized City Bus Service (CCBS) can reduce the traffic congestion and environmental pollution that caused by the increasing in private cars, effectively. This study aims to extract the potential bus lines and each line's passenger density of CCBS by mining the public transport big data. The datasets used in this study are mainly Smart Card Data (SCD) and bus GPS data of Qingdao, China, from October 11th and November 7th 2015. Firstly, we compute the temporal-origin-destination (TOD) of passengers by mining SCD and bus GPS data. Compared with the traditional OD, TOD not only has the spatial location, but also contains the trip's boarding time. Secondly, based on the traditional DBSCAN algorithm, we put forwards an algorithm, named TOD-DBSCAN, combined with the spatial-temporal features of TOD.TOD-DBSCAN is used to cluster the TOD trajectories in peak hours of all working days. Then, we define two variables P and N to describe the possibility and passenger destiny of a potential CCBS line. P is the probability of the CCBS line. And N represents the potential passenger destiny of the line. Lastly, we visualize the potential CCBS lines extracted by our procedure on the map and analyse relationship between potential CCBS lines and the urban spatial structure.

  11. Plasma transport in the interplanetary space: Percolation and anomalous diffusion of magnetic-field lines

    Energy Technology Data Exchange (ETDEWEB)

    Zimbardo, G.; Veltri, P. [Arcavacata di Rende, Cosenza, Univ. della Calabria (Italy). Dipt. di Fisica

    1997-11-01

    The magnetic fluctuations due to, e.g., magnetohydrodynamic turbulence cause a magnetic-field line random walk that influences many cosmic plasma phenomena. The results of a three-dimensional numerical simulation of a turbulent magnetic field in plane geometry are presented here. Magnetic percolation, Levy flights, and non-Gaussian random walk of the magnetic-field lines are found for moderate perturbation levels. In such a case plasma transport can be anomalous, i.e., either super diffusive or sub diffusive. Increasing the perturbation level a Gaussian diffusion regime is attained. The implications on the structure of the electron fore shock and of planetary magneto pauses are discussed.

  12. Particle Transport along Magnetic Null Lines as Sputter or Antihydrogen Source

    Science.gov (United States)

    Lane, R. A.; Ordonez, C. A.

    Particle transport along null magnetic lines is investigated using classical trajectory Monte Carlo simulations and described as a traveling wave and through diffusion equations. A magnetic null line is defined as a one-dimensional region where the magnetic field magnitude is zero. This region may take any shape in three-dimensional space. The field used in the simulations is generated by two infinite wires of negligible thickness carrying identical current and separated by a small distance. Thus, an infinite magnetic null line exists directly between the wires. The particle trajectories are simulated by solving the equations of motion for each simulated particle of a mono- energetic set. Each is considered individually, with all trajectories starting from the same position along the null line. Each trajectory is simulated until it reaches a specified distance from the initial point or a maximum time elapses. The simulation is repeated using a full set for multiple endpoints and maximum times for ten different amounts of current in the wires. Each current value is selected so that no particles can travel more than seven times the distance between the wires from the null line. The fraction of particles that reach the endpoint in a given time is calculated and used to describe particle transport parallel to the null line. The results are given in normalized, dimensionless units and their possible applications as an antihydrogen source and use in ultra-high purity sputter are discussed. The results are used to find the conditions necessary to obtain a steady and uniform particle flux suitable for ultra-high purity sputter, assuming that plasma is generated near the null line.

  13. Path integral approach for electron transport in disturbed magnetic field lines

    Energy Technology Data Exchange (ETDEWEB)

    Kanno, Ryutaro; Nakajima, Noriyoshi; Takamaru, Hisanori

    2002-05-01

    A path integral method is developed to investigate statistical property of an electron transport described as a Langevin equation in a statically disturbed magnetic field line structure; especially a transition probability of electrons strongly tied to field lines is considered. The path integral method has advantages that 1) it does not include intrinsically a growing numerical error of an orbit, which is caused by evolution of the Langevin equation under a finite calculation accuracy in a chaotic field line structure, and 2) it gives a method of understanding the qualitative content of the Langevin equation and assists to expect statistical property of the transport. Monte Carlo calculations of the electron distributions under both effects of chaotic field lines and collisions are demonstrated to comprehend above advantages through some examples. The mathematical techniques are useful to study statistical properties of various phenomena described as Langevin equations in general. By using parallel generators of random numbers, the Monte Carlo scheme to calculate a transition probability can be suitable for a parallel computation. (author)

  14. Transport line for a multi-staged laser-plasma acceleration: DACTOMUS

    Science.gov (United States)

    Chancé, Antoine; Delferrière, Olivier; Schwindling, Jérôme; Bruni, Christelle; Delerue, Nicolas; Specka, Arnd; Cros, Brgitte; Maynard, Gillies; Paradkar, Bhooshan S.; Mora, Patrick

    2014-03-01

    Laser-plasma acceleration is one of the most promising techniques to reach very high acceleration gradients up to a few hundreds of GeV/m. In order to push this acceleration scheme in the domain of the very high energies, the CILEX project was launched with the laser APOLLON. One of the main topics of this project is to study multi-staged acceleration. It consists in generating and pre-accelerating electrons in a first laser-plasma stage, to transport them up to a second stage where the electrons are accelerated again thanks to another laser pulse. The DACTOMUS project, based on a collaboration CEA-IRFU, CEA-IRAMIS, LAL, LPGP, LULI and LLR, aims at the study and realization of such a transfer line between these two stages. Firstly, a prototype will be developed and tested by the groups of CEA-IRAMIS-SPAM, LPGP, and LULI on the UHI100 facility (CEA-SPAM). This collaboration must enable to realize the first acceleration stage. For the transport line prototype, the main difficulties are to realize a very compact and energy accepting line with diagnostics to characterize the electron beam. We will present here the optics of this line, its performances and the inserted diagnostics.

  15. Transport line for a multi-staged laser-plasma acceleration: DACTOMUS

    Energy Technology Data Exchange (ETDEWEB)

    Chancé, Antoine, E-mail: antoine.chance@cea.fr [CEA, IRFU, SACM, Centre de Saclay, F-91191 Gif-sur-Yvette (France); Delferrière, Olivier; Schwindling, Jérôme [CEA, IRFU, SACM, Centre de Saclay, F-91191 Gif-sur-Yvette (France); Bruni, Christelle; Delerue, Nicolas [LAL, UMR9607, CNRS and Université Paris Sud, Orsay (France); Specka, Arnd [LLR, UMR7638, CNRS and Ecole Polytechnique, Palaiseau (France); Cros, Brgitte; Maynard, Gillies; Paradkar, Bhooshan S. [LPGP, UMR8578, CNRS and Université Paris Sud, Orsay (France); Mora, Patrick [CPhT, UMR7644, CNRS and Ecole Polytechnique, Palaiseau (France)

    2014-03-11

    Laser-plasma acceleration is one of the most promising techniques to reach very high acceleration gradients up to a few hundreds of GeV/m. In order to push this acceleration scheme in the domain of the very high energies, the CILEX project was launched with the laser APOLLON. One of the main topics of this project is to study multi-staged acceleration. It consists in generating and pre-accelerating electrons in a first laser-plasma stage, to transport them up to a second stage where the electrons are accelerated again thanks to another laser pulse. The DACTOMUS project, based on a collaboration CEA-IRFU, CEA-IRAMIS, LAL, LPGP, LULI and LLR, aims at the study and realization of such a transfer line between these two stages. Firstly, a prototype will be developed and tested by the groups of CEA-IRAMIS-SPAM, LPGP, and LULI on the UHI100 facility (CEA-SPAM). This collaboration must enable to realize the first acceleration stage. For the transport line prototype, the main difficulties are to realize a very compact and energy accepting line with diagnostics to characterize the electron beam. We will present here the optics of this line, its performances and the inserted diagnostics.

  16. FUZZY COMPREHENSIVE EVALUATION OF CONTAINER TRANSPORTATION MODES ALONG THE CHANGJIANG RIVER MAIN LINE AND ITS DELTA AREA

    Institute of Scientific and Technical Information of China (English)

    ZHANG Shi-yu; XIAO Han-liang

    2003-01-01

    The Changjiang River (Yangtze) is one of the fastest growth areas of container transportation in Chi-na. Rail, road and water transportation have competed against each other for container transportation in the Chang-jiang River main line and its delta area. It is of significance to assess these different transportation modes scientifi-cally in order to organize container transportation efficiently in this area and make decision for integral plan and construction of transportation system in this area. This paper outlines application of fuzzy comprehensive evaluation to appraise different modes of typical direction of containers. Twelve assessment indexes were decided. Membership functions were formulated. Evaluation results indicated that road transportation was optimal mode in the Changjiang River delta area, however water transportation was the primary way in the Changjiang River main line.

  17. AP Music Theory Applied

    Science.gov (United States)

    Spieker, Matthew H.

    2016-01-01

    Some American high schools include Advanced Placement (AP) Music Theory within their course offerings. Students who pass the AP exam can receive college credit either as a music or humanities credit. An AP class, however, offers music students more than future college credit; it ultimately improves musicianship skills and promotes deeper…

  18. Poly(acrylic acid) coating induced 2-line ferrihydrite nanoparticle transport in saturated porous media

    Science.gov (United States)

    Xiang, Aishuang; Yan, Weile; Koel, Bruce E.; Jaffé, Peter R.

    2013-07-01

    Iron oxide and iron nanoparticles (NPs) have been used effectively for environmental remediation, but are limited in their applications by strong retention in groundwater-saturated porous media. For example, delivery of NPs to large groundwater reservoirs would require large numbers of injection wells. To address this problem, we have explored polymer coatings as a surface engineering strategy to enhance transport of oxide nanoparticles in porous media. We report here on our studies of 2-line ferrihydrite NPs and the influence of poly (acrylic acid) (PAA) polymer coatings on the colloidal stability and transport in natural sand-packed column tests simulating flow in groundwater-saturated porous media. Measurements were also made of zeta potential, hydrodynamic diameter, and polymer adsorption and desorption properties. The coated NPs have a diameter range of 30-500 nm. We found that NP transport was improved by PAA coating and that the transport properties could be tuned by adjusting the polymer concentration. Our results demonstrate that a high stability of oxide particles and improved transport can be achieved in groundwater-saturated porous media by introducing negatively charged polyelectrolytes and optimizing polymer concentrations.

  19. Poly(acrylic acid) coating induced 2-line ferrihydrite nanoparticle transport in saturated porous media

    Energy Technology Data Exchange (ETDEWEB)

    Xiang, Aishuang [Princeton University, Chemical and Biological Engineering Department (United States); Yan, Weile [Texas Tech University, Civil and Environmental Engineering (United States); Koel, Bruce E., E-mail: bkoel@princeton.edu [Princeton University, Chemical and Biological Engineering Department (United States); Jaffe, Peter R., E-mail: jaffe@princeton.edu [Princeton University, Civil and Environmental Engineering Department (United States)

    2013-07-15

    Iron oxide and iron nanoparticles (NPs) have been used effectively for environmental remediation, but are limited in their applications by strong retention in groundwater-saturated porous media. For example, delivery of NPs to large groundwater reservoirs would require large numbers of injection wells. To address this problem, we have explored polymer coatings as a surface engineering strategy to enhance transport of oxide nanoparticles in porous media. We report here on our studies of 2-line ferrihydrite NPs and the influence of poly (acrylic acid) (PAA) polymer coatings on the colloidal stability and transport in natural sand-packed column tests simulating flow in groundwater-saturated porous media. Measurements were also made of zeta potential, hydrodynamic diameter, and polymer adsorption and desorption properties. The coated NPs have a diameter range of 30-500 nm. We found that NP transport was improved by PAA coating and that the transport properties could be tuned by adjusting the polymer concentration. Our results demonstrate that a high stability of oxide particles and improved transport can be achieved in groundwater-saturated porous media by introducing negatively charged polyelectrolytes and optimizing polymer concentrations.

  20. Diffusive Heat Transport in Budyko's Energy Balance Climate Model with a Dynamic Ice Line

    CERN Document Server

    Walsh, James

    2016-01-01

    M. Budyko and W. Sellers independently introduced seminal energy balance climate models in 1969, each with a goal of investigating the role played by positive ice albedo feedback in climate dynamics. In this paper we replace the relaxation to the mean horizontal heat transport mechanism used in the models of Budyko and Sellers with diffusive heat transport. We couple the resulting surface temperature equation with an equation for movement of the edge of the ice sheet (called the ice line), recently introduced by E. Widiasih. We apply the spectral method to the temperature-ice line system and consider finite approximations. We prove there exists a stable equilibrium solution with a small ice cap, and an unstable equilibrium solution with a large ice cap, for a range of parameter values. If the diffusive transport is too efficient, however, the small ice cap disappears and an ice free Earth becomes a limiting state. In addition, we analyze a variant of the coupled diffusion equations appropriate as a model for ...

  1. A Central Role for JNK/AP-1 Pathway in the Pro-Oxidant Effect of Pyrrolidine Dithiocarbamate through Superoxide Dismutase 1 Gene Repression and Reactive Oxygen Species Generation in Hematopoietic Human Cancer Cell Line U937.

    Science.gov (United States)

    Riera, Humberto; Afonso, Valéry; Collin, Pascal; Lomri, Abderrahim

    2015-01-01

    Pyrrolidine dithiocarbamate (PDTC) known as antioxidant and specific inhibitor of NF-κB was also described as pro-oxidant by inducing cell death and reactive oxygen species (ROS) accumulation in cancer. However, the mechanism by which PDTC indices its pro-oxidant effect is unknown. Therefore, we aimed to evaluate the effect of PDTC on the human Cu/Zn superoxide dismutase 1 (SOD1) gene transcription in hematopoietic human cancer cell line U937. We herein show for the first time that PDTC decreases SOD1 transcripts, protein and promoter activity. Furthermore, SOD1 repression by PDTC was associated with an increase in oxidative stress as evidenced by ROS production. Electrophoretic mobility-shift assays (EMSA) show that PDTC increased binding of activating protein-1 (AP-1) in dose dependent-manner suggesting that the MAPkinase up-stream of AP-1 is involved. Ectopic NF-κB p65 subunit overexpression had no effect on SOD1 transcription. In contrast, in the presence of JNK inhibitor (SP600125), p65 induced a marked increase of SOD1 promoter, suggesting that JNK pathway is up-stream of NF-κB signaling and controls negatively its activity. Indeed, using JNK deficient cells, PDTC effect was not observed nether on SOD1 transcription or enzymatic activity, nor on ROS production. Finally, PDTC represses SOD1 in U937 cells through JNK/c-Jun phosphorylation. Taken together, these results suggest that PDTC acts as pro-oxidant compound in JNK/AP-1 dependent-manner by repressing the superoxide dismutase 1 gene leading to intracellular ROS accumulation.

  2. A Central Role for JNK/AP-1 Pathway in the Pro-Oxidant Effect of Pyrrolidine Dithiocarbamate through Superoxide Dismutase 1 Gene Repression and Reactive Oxygen Species Generation in Hematopoietic Human Cancer Cell Line U937.

    Directory of Open Access Journals (Sweden)

    Humberto Riera

    Full Text Available Pyrrolidine dithiocarbamate (PDTC known as antioxidant and specific inhibitor of NF-κB was also described as pro-oxidant by inducing cell death and reactive oxygen species (ROS accumulation in cancer. However, the mechanism by which PDTC indices its pro-oxidant effect is unknown. Therefore, we aimed to evaluate the effect of PDTC on the human Cu/Zn superoxide dismutase 1 (SOD1 gene transcription in hematopoietic human cancer cell line U937. We herein show for the first time that PDTC decreases SOD1 transcripts, protein and promoter activity. Furthermore, SOD1 repression by PDTC was associated with an increase in oxidative stress as evidenced by ROS production. Electrophoretic mobility-shift assays (EMSA show that PDTC increased binding of activating protein-1 (AP-1 in dose dependent-manner suggesting that the MAPkinase up-stream of AP-1 is involved. Ectopic NF-κB p65 subunit overexpression had no effect on SOD1 transcription. In contrast, in the presence of JNK inhibitor (SP600125, p65 induced a marked increase of SOD1 promoter, suggesting that JNK pathway is up-stream of NF-κB signaling and controls negatively its activity. Indeed, using JNK deficient cells, PDTC effect was not observed nether on SOD1 transcription or enzymatic activity, nor on ROS production. Finally, PDTC represses SOD1 in U937 cells through JNK/c-Jun phosphorylation. Taken together, these results suggest that PDTC acts as pro-oxidant compound in JNK/AP-1 dependent-manner by repressing the superoxide dismutase 1 gene leading to intracellular ROS accumulation.

  3. Initial diagnostics commissioning results for the APS injector subsystems

    Science.gov (United States)

    Lumpkin, A.; Chung, Y.; Kahana, E.; Patterson, D.; Sellyey, W.; Smith, T.; Wang, X.

    1995-05-01

    In recent months the first beams have been introduced into the various injector subsystems of the Advanced Photon Source (APS). An overview will be given of the diagnostics results on beam profiling, beam position monitors (BPMs), loss rate monitors (LRMs), current monitors (CMs), and photon monitors on the low energy transport lines, positron accumulator ring (PAR), and injector synchrotron (IS). Initial measurements have been done with electron beams at energies from 250 to 450 MeV and 50 to 400 pC per macrobunch. Operations in single turn and stored beam conditions were diagnosed in the PAR and IS.

  4. Transmission line model for strained quantum well lasers including carrier transport and carrier heating effects.

    Science.gov (United States)

    Xia, Mingjun; Ghafouri-Shiraz, H

    2016-03-01

    This paper reports a new model for strained quantum well lasers, which are based on the quantum well transmission line modeling method where effects of both carrier transport and carrier heating have been included. We have applied this new model and studied the effect of carrier transport on the output waveform of a strained quantum well laser both in time and frequency domains. It has been found that the carrier transport increases the turn-on, turn-off delay times and damping of the quantum well laser transient response. Also, analysis in the frequency domain indicates that the carrier transport causes the output spectrum of the quantum well laser in steady state to exhibit a redshift which has a narrower bandwidth and lower magnitude. The simulation results of turning-on transients obtained by the proposed model are compared with those obtained by the rate equation laser model. The new model has also been used to study the effects of pump current spikes on the laser output waveforms properties, and it was found that the presence of current spikes causes (i) wavelength blueshift, (ii) larger bandwidth, and (iii) reduces the magnitude and decreases the side-lobe suppression ratio of the laser output spectrum. Analysis in both frequency and time domains confirms that the new proposed model can accurately predict the temporal and spectral behaviors of strained quantum well lasers.

  5. Road and Street Centerlines - RAMPS_INDOT_IN: Ramp System in Indiana (Indiana Department of Transportation, Line Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — RAMPS_INDOT_IN is a line shapefile that contains all ramps in Indiana, provided by personnel of Indiana Department of Transportation (INDOT), Business Information...

  6. Mass transport and direction dependent battery modeling for accurate on-line power capability prediction

    Energy Technology Data Exchange (ETDEWEB)

    Wiegman, H.L.N. [General Electric Corporate Research and Development, Schenectady, NY (United States)

    2000-07-01

    Some recent advances in battery modeling were discussed with reference to on-line impedance estimates and power performance predictions for aqueous solution, porous electrode cell structures. The objective was to determine which methods accurately estimate a battery's internal state and power capability while operating a charge and sustaining a hybrid electric vehicle (HEV) over a wide range of driving conditions. The enhancements to the Randles-Ershler equivalent electrical model of common cells with lead-acid, nickel-cadmium and nickel-metal hydride chemistries were described. This study also investigated which impedances are sensitive to boundary layer charge concentrations and mass transport limitations. Non-linear impedances were shown to significantly affect the battery's ability to process power. The main advantage of on-line estimating a battery's impedance state and power capability is that the battery can be optimally sized for any application. refs., tabs., figs., append.

  7. Design of the low energy beam transport line for the China spallation neutron source

    Institute of Scientific and Technical Information of China (English)

    LI Jin-Hai; OUYANG Hua-Fu; FU Shi-Nian; ZHANG Sua-Shun; HE Wei

    2008-01-01

    The design of the China Spallation Neutron Source (CSNS) low-energy beam transport (LEBT) line, which locates between the ion source and the radio-frequency quadrupole (RFQ), has been completed with the TRACE3D code. The design aims at perfect matching, primary chopping, a small emittance growth and sufficient space for beam diagnostics. The line consists of three solenoids, three vacuum chambers, two steering magnets and a pre-chopper. The total length of LEBT is about 1.74 m. This LEBT is designed to transfer 20 mA of H-pulsed beam from the ion source to the RFQ. An induction cavity is adopted as the pre-chopper.The electrostatic octupole steerer is discussed as a candidate. A four-quadrant aperture for beam scraping and beam position monitoring is designed.

  8. Transport of Coherent VUV Radiation to Muon U-Line for Ultra Slow Muon Microscope

    Science.gov (United States)

    Nakamura, Jumpei; Oishi, Yu; Saito, Norihito; Miyazaki, Koji; Yokoyama, Koji; Okamura, Kotaro; Makimura, Shunsuke; Miyake, Yasuhiro; Nagatomo, Takashi; Strasser, Patrick; Ikedo, Yutaka; Tomono, Dai; Shimomura, Koichiro; Wada, Satoshi; Kawamura, Naritoshi; Koda, Akihiro; Nishiyama, Kusuo

    At U-line/MUSE/J-PARC, thermal muonium generation and laser resonant ionization process are required to get Ultra Slow Muon Beam. Laser radiation sources for the laser resonant ionization have been developed at RIKEN, and installed in a laser cabin. The laser radiations are introduced to Muon U-line by a transport system of the laser pulses, which consists of a VUV steering chamber and a NO gas cell. Coherent VUV radiation can be separated from two "bright" fundamental radiations for wave mixing, and 355 nm radiation is guided to the muonium production chamber at nearly same angle as VUV radiation, at the VUV steering chamber which is an ultrahigh vacuum chamber. The NO gas cell consist of the parallel plate ionization chamber with nitrogen monoxide molecules for measuring intensity of the VUV radiation.

  9. Fiberglass distribution poles: A technical-economic solution for electric energy transport lines

    Energy Technology Data Exchange (ETDEWEB)

    Souto Rodriguez, E.L. [Norpost S.A., Lugones (Spain). Poligono Industrial de Silvota; Balust, J.S. [Union Electrica Fenosa, Madrid (Spain)

    1995-11-01

    In Spain, building on the experiences from the more developed nations, a research project on an economic fiberglass distribution pole for electric energy transport lines was started. The paper describes the studies carried out in three phases: general survey, characteristics and properties, and economic aspects. The characteristics of the poles which are described are: light weight, electrical properties, easy installation, safety, low maintenance, longevity, vibration damping, environmental impacts, and mechanical properties. The economic analysis showed that although fiberglass reinforced composites cost more to procure, their life-cycle cost is less due to low maintenance.

  10. Functional Coding Variation in Recombinant Inbred Mouse Lines Reveals Novel Serotonin Transporter-Associated Phenotypes

    Energy Technology Data Exchange (ETDEWEB)

    Carneiro, Ana [Vanderbilt University; Airey, David [University of Tennessee Health Science Center, Memphis; Thompson, Brent [Vanderbilt University; Zhu, C [Vanderbilt University; Rinchik, Eugene M [ORNL; Lu, Lu [University of Tennessee Health Science Center, Memphis; Chesler, Elissa J [ORNL; Erikson, Keith [University of North Carolina; Blakely, Randy [Vanderbilt University

    2009-01-01

    The human serotonin (5-hydroxytryptamine, 5-HT) transporter (hSERT, SLC6A4) figures prominently in the etiology or treatment of many prevalent neurobehavioral disorders including anxiety, alcoholism, depression, autism and obsessive-compulsive disorder (OCD). Here we utilize naturally occurring polymorphisms in recombinant inbred (RI) lines to identify novel phenotypes associated with altered SERT function. The widely used mouse strain C57BL/6J, harbors a SERT haplotype defined by two nonsynonymous coding variants (Gly39 and Lys152 (GK)). At these positions, many other mouse lines, including DBA/2J, encode Glu39 and Arg152 (ER haplotype), assignments found also in hSERT. Synaptosomal 5-HT transport studies revealed reduced uptake associated with the GK variant. Heterologous expression studies confirmed a reduced SERT turnover rate for the GK variant. Experimental and in silico approaches using RI lines (C57Bl/6J X DBA/2J=BXD) identifies multiple anatomical, biochemical and behavioral phenotypes specifically impacted by GK/ER variation. Among our findings are multiple traits associated with anxiety and alcohol consumption, as well as of the control of dopamine (DA) signaling. Further bioinformatic analysis of BXD phenotypes, combined with biochemical evaluation of SERT knockout mice, nominates SERT-dependent 5-HT signaling as a major determinant of midbrain iron homeostasis that, in turn, dictates ironregulated DA phenotypes. Our studies provide a novel example of the power of coordinated in vitro, in vivo and in silico approaches using murine RI lines to elucidate and quantify the system-level impact of gene variation.

  11. Advanced Photon Source (APS)

    Data.gov (United States)

    Federal Laboratory Consortium — The Advanced Photon Source (APS) at the U.S. Department of Energy's Argonne National Laboratoryprovides this nation's (in fact, this hemisphere's) brightest storage...

  12. Reconstitution of bile acid transport in the rat hepatoma McArdle RH-7777 cell line.

    Science.gov (United States)

    Torchia, E C; Shapiro, R J; Agellon, L B

    1996-07-01

    The liver recovers bile acids from the portal circulation primarily via an active process that is dependent on sodium ions. Hepatocytes lose the ability to transport bile acids in culture, and, in liver-derived permanent cell lines, this ability is severely reduced or absent. To study the importance of bile acids in regulating liver-specific functions (e.g., cellular bile acid and cholesterol metabolism), we have re-established active bile acid transport in cultured cells. The complementary DNA (cDNA) encoding the rat sodium/taurocholate cotransporting polypeptide (ntcp) was placed under the control of a cytomegalovirus promoter and transfected into the rat hepatoma cell line, McArdle RH-7777. Transfected cells were screened for the ability to take up [3H]-taurocholate. Clones that displayed the ability to take up taurocholate were expanded (designated McNtcp) and further characterized. The apparent Michaelis constant (Km) for taurocholate uptake was similar among the different clones. The observed maximum velocity (Vmax), however, differed and was positively correlated with the abundance of recombinant ntcp messenger RNA (mRNA). The highest level of taurocholate uptake activity observed in McNtcp cells was comparable with that of freshly isolated hepatocytes. Efflux of accumulated taurocholate from McNtcp cells proceeded in a manner similar to primary hepatocytes, indicating that McArdle RH-7777 cells have retained the ability to secrete bile acids. Moreover, taurocholate uptake in McNtcp cells was inhibited by other bile acid species. Based on the observed kinetic parameters, the reconstituted McArdle RH-7777 cells mimic the ability of primary hepatocytes to transport bile acids.

  13. Generic effluent monitoring system certification for AP-40 exhauster stack

    Energy Technology Data Exchange (ETDEWEB)

    Glissmeyer, J.A.; Davis, W.E.; Bussell, J.H.; Maughan, A.D.

    1997-09-01

    Tests were conducted to verify that the Generic Effluent Monitoring System (GEMS), as applied to the AP-40 exhauster stack, meets all applicable regulatory performance criteria for air sampling systems at nuclear facilities. These performance criteria address both the suitability of the air sampling probe location and the transport of the sample to the collection devices. The criteria covering air sampling probe location ensure that the contaminants in the stack are well mixed with the airflow at the probe location such that the extracted sample represents the whole. The sample transport criteria ensure that the sampled contaminants are quantitatively delivered to the collection device. The specific performance criteria are described in detail in the report. The tests demonstrated that the GEMS/AP-40 system meets all applicable performance criteria. The contaminant mixing tests were conducted by Pacific Northwest National Laboratory (PNNL) at the wind tunnel facility, 331-H Building, using a mockup of the actual stack. The particle sample transport tests were conducted by PNNL at the Numatec Hanford Company`s 305 Building. The AP-40 stack is typical of several 10-in. diameter stacks that discharge the filtered ventilation air from tank farms at the U.S. Department of Energy`s Hanford Site in Richland, Washington. The GEMS design features a probe with a single shrouded sampling nozzle, a sample delivery line, and sample collection system. The collection system includes a filter holder to collect the sample of record and an in-line detector head and filter for monitoring beta radiation-emitting particles. Unrelated to the performance criteria, it was found that the record sample filter holder exhibited symptoms of sample bypass around the particle collection filter. This filter holder should either be modified or replaced with a different type. 10 refs., 8 figs., 6 tabs.

  14. Optimization of solenoid based low energy beam transport line for high current H+ beams

    Science.gov (United States)

    Pande, R.; Singh, P.; Rao, S. V. L. S.; Roy, S.; Krishnagopal, S.

    2015-02-01

    A 20 MeV, 30 mA CW proton linac is being developed at BARC, Mumbai. This linac will consist of an ECR ion source followed by a Radio Frequency Quadrupole (RFQ) and Drift tube Linac (DTL). The low energy beam transport (LEBT) line is used to match the beam from the ion source to the RFQ with minimum beam loss and increase in emittance. The LEBT is also used to eliminate the unwanted ions like H2+ and H3+ from entering the RFQ. In addition, space charge compensation is required for transportation of such high beam currents. All this requires careful design and optimization. Detailed beam dynamics simulations have been done to optimize the design of the LEBT using the Particle-in-cell code TRACEWIN. We find that with careful optimization it is possible to transport a 30 mA CW proton beam through the LEBT with 100% transmission and minimal emittance blow up, while at the same time suppressing unwanted species H2+ and H3+ to less than 3.3% of the total beam current.

  15. Noble internal transport barriers and radial subdiffusion of toroidal magnetic lines

    Energy Technology Data Exchange (ETDEWEB)

    Misguich, J.H.; Reuss, J.D. [Association Euratom-CEA sur la Fusion, CEA/DSM/DRFC, 13 - Saint Paul lez Durance (France); Constantinescu, D.; Steinbrecher, G. [Association Euratom-N.A.S.T.I., Dept. of Physics, University of Craiova (Romania); Vlad, M.; Spineanu, F. [Association Euratom-N.A.S.T.I., National Institute of Laser, Plasma and Radiation Physics, Bucharest (Romania); Weyssow, B.; Balescu, R. [Association Euratom-Etat Belge sur la Fusion, Universite Libre de Bruxelles (Belgium)

    2002-02-01

    Internal transport barriers (ITB's) observed in tokamaks are described by a purely magnetic approach. Magnetic line motion in toroidal geometry with broken magnetic surfaces is studied from a previously derived Hamiltonian map in situation of incomplete chaos. This appears to reproduce in a realistic way the main features of a tokamak, for a given safety factor profile and in terms of a single parameter L representing the amplitude of the magnetic perturbation. New results are given concerning the Shafranov shift as function of L. For small values of L, closed magnetic surfaces exist (KAM tori) and island chains begin to appear on rational surfaces for higher values of L, with chaotic zones around hyperbolic points, as expected. Single trajectories of magnetic line motion indicate the persistence of a central protected plasma core, surrounded by a chaotic shell enclosed in a double-sided transport barrier. Magnetic lines which succeed to escape across this barrier begin to wander in a wide chaotic sea extending up to a very robust barrier (as long as L{<=}1). For values of L{>=}1, above the escape threshold, most magnetic lines succeed to escape out of the external barrier which has become a permeable Cantorus. Statistical analysis of a large number of trajectories, representing the evolution of a bunch of magnetic lines, indicate that the flux variable {psi} asymptotically grows in a diffuse manner as (L{sup 2}t) with a L{sup 2} scaling as expected, but that the average radial position r{sub m}(t) asymptotically grows as (L{sup 2}t){sup 1/4} while the mean square displacement around this average radius asymptotically grows in a sub-diffusive manner as (L{sup 2}t){sup 1/2}. This result shows the slower dispersion in the present incomplete chaotic regime, which is different from the usual quasilinear diffusion in completely chaotic situations. For physical times t{sub {phi}} of the order of the escape time defined by x{sub m}(t{sub {phi}}) {approx}1, the motion

  16. APS and Open Access

    Science.gov (United States)

    2011-03-01

    The movement toward Open Access continues to gain momentum. A brief review of APS efforts in this area will be presented by APS Editor in Chief, Gene Sprouse. Editors from Physical Review A, B, E, Focus, Letters, and X, Reviews of Modern Physics, and Physics will address your questions about publishing in this evolving environment.

  17. Quantitative assessment of direct and indirect landslide risk along transportation lines in southern India

    Science.gov (United States)

    Jaiswal, P.; van Westen, C. J.; Jetten, V.

    2010-06-01

    A quantitative approach for landslide risk assessment along transportation lines is presented and applied to a road and a railway alignment in the Nilgiri hills in southern India. The method allows estimating direct risk affecting the alignments, vehicles and people, and indirect risk resulting from the disruption of economic activities. The data required for the risk estimation were obtained from historical records. A total of 901 landslides were catalogued initiating from cut slopes along the railway and road alignment. The landslides were grouped into three magnitude classes based on the landslide type, volume, scar depth, run-out distance, etc and their probability of occurrence was obtained using frequency-volume distribution. Hazard, for a given return period, expressed as the number of landslides of a given magnitude class per kilometre of cut slopes, was obtained using Gumbel distribution and probability of landslide magnitude. In total 18 specific hazard scenarios were generated using the three magnitude classes and six return periods (1, 3, 5, 15, 25, and 50 years). The assessment of the vulnerability of the road and railway line was based on damage records whereas the vulnerability of different types of vehicles and people was subjectively assessed based on limited historic incidents. Direct specific loss for the alignments (railway line and road), vehicles (train, bus, lorry, car and motorbike) was expressed in monetary value (US), and direct specific loss of life of commuters was expressed in annual probability of death. Indirect specific loss (US) derived from the traffic interruption was evaluated considering alternative driving routes, and includes losses resulting from additional fuel consumption, additional travel cost, loss of income to the local business, and loss of revenue to the railway department. The results indicate that the total loss, including both direct and indirect loss, from 1 to 50 years return period, varies from US 90 840 to US

  18. Quantitative assessment of direct and indirect landslide risk along transportation lines in southern India

    Directory of Open Access Journals (Sweden)

    P. Jaiswal

    2010-06-01

    Full Text Available A quantitative approach for landslide risk assessment along transportation lines is presented and applied to a road and a railway alignment in the Nilgiri hills in southern India. The method allows estimating direct risk affecting the alignments, vehicles and people, and indirect risk resulting from the disruption of economic activities. The data required for the risk estimation were obtained from historical records. A total of 901 landslides were catalogued initiating from cut slopes along the railway and road alignment. The landslides were grouped into three magnitude classes based on the landslide type, volume, scar depth, run-out distance, etc and their probability of occurrence was obtained using frequency-volume distribution. Hazard, for a given return period, expressed as the number of landslides of a given magnitude class per kilometre of cut slopes, was obtained using Gumbel distribution and probability of landslide magnitude. In total 18 specific hazard scenarios were generated using the three magnitude classes and six return periods (1, 3, 5, 15, 25, and 50 years. The assessment of the vulnerability of the road and railway line was based on damage records whereas the vulnerability of different types of vehicles and people was subjectively assessed based on limited historic incidents. Direct specific loss for the alignments (railway line and road, vehicles (train, bus, lorry, car and motorbike was expressed in monetary value (US$, and direct specific loss of life of commuters was expressed in annual probability of death. Indirect specific loss (US$ derived from the traffic interruption was evaluated considering alternative driving routes, and includes losses resulting from additional fuel consumption, additional travel cost, loss of income to the local business, and loss of revenue to the railway department. The results indicate that the total loss, including both direct and indirect loss, from 1 to 50 years return period, varies from

  19. Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2.

    Science.gov (United States)

    Weerachayaphorn, Jittima; Pajor, Ana M

    2008-04-01

    Citric acid cycle intermediates are absorbed from the gastrointestinal tract through carrier-mediated mechanisms, although the transport pathways have not been clearly identified. This study examines the transport of citric acid cycle intermediates in the Caco-2 human colon carcinoma cell line, often used as a model of small intestine. Inulin was used as an extracellular volume marker instead of mannitol since the apparent volume measured with mannitol changed with time. The results show that Caco-2 cells contain at least three distinct transporters, including the Na+-dependent di- and tricarboxylate transporters, NaDC1 and NaCT, and one or more sodium-independent pathways, possibly involving organic anion transporters. Succinate transport is mediated mostly by Na+-dependent pathways, predominantly by NaDC1, but with some contribution by NaCT. RT-PCR and functional characteristics verified the expression of these transporters in Caco-2 cells. In contrast, citrate transport in Caco-2 cells occurs by a combination of Na+-independent pathways, possibly mediated by an organic anion transporter, and Na+-dependent mechanisms. The non-metabolizable dicarboxylate, methylsuccinate, is also transported by a combination of Na+-dependent and -independent pathways. In conclusion, we find that multiple pathways are involved in the transport of di- and tricarboxylates by Caco-2 cells. Since many of these pathways are not found in human intestine, this model may be best suited for studying Na+-dependent transport of succinate by NaDC1.

  20. THE COMMISSIONING PLAN FOR THE SPALLATION NEUTRON SOURCE RING AND TRANSPORT LINES.

    Energy Technology Data Exchange (ETDEWEB)

    RAPARIA,D.BLASKIEWICZ,M.LEE,Y.Y.WEI,J.ET AL.

    2004-03-10

    The Spallation Neutron Source (SNS) accelerator systems will provide a 1 GeV, 1.44 MW proton beam to a liquid mercury target for neutron production. In order to satisfy the accelerator systems' portion of the Critical Decision 4 (CD-4) commissioning goal (which marks the completion of the construction phase of the project), a beam pulse with intensity greater than 1 x 10{sup 13} protons must be accumulated in the ring, extracted in a single turn and delivered to the target. A commissioning plan has been formulated for bringing into operation and establishing nominal operating conditions for the various ring and transport line subsystems as well as for establishing beam conditions and parameters which meet the commissioning goal.

  1. Quality control and patient dosimetry in digital radiology. On line system: new features and transportability.

    Science.gov (United States)

    Vano, E; Ten, J I; Fernandez, J M; Prieto, C; Ordiales, J M; Martinez, D

    2008-01-01

    New features have been added to a system (QCONLINE) for auditing patient dosimetric and technical parameters 'on line', working on a digital radiology department and using the information contained in the DICOM header of some modalities. The audit of other parameters than patient doses have been included, setting alarm conditions to alert on malfunction of the X-ray system or bad operation modes, in addition to the evaluation of patient doses. A new module to analyse, collect and process the relevant information transferred by the modality performed procedure step (MPPS) service has been launched. Several examples with the exploitation of the new features are presented. The transportability of the system has been tested in two remote hospitals during several months. The new MPPS module has demonstrated to be a good tool to complement the information existing in the DICOM header. The system allows to help in the optimisation of digital radiology departments managing patient dosimetry and procedure data in real time.

  2. Comparison of implicit and symbolic implicit Monte Carlo line transport with frequency weight vector extension

    Science.gov (United States)

    McKinley, Michael Scott; Brooks, Eugene D., III; Szoke, Abraham

    2003-07-01

    We compare the implicit Monte Carlo (IMC) technique to the symbolic IMC (SIMC) technique, with and without weight vectors in frequency space, for time-dependent line transport in the presence of collisional pumping. We examine the efficiency and accuracy of the IMC and SIMC methods for test problems involving the evolution of a collisionally pumped trapping problem to its steady-state, the surface heating of a cold medium by a beam, and the diffusion of energy from a localized region that is collisionally pumped. The importance of spatial biasing and teleportation for problems involving high opacity is demonstrated. Our numerical solution, along with its associated teleportation error, is checked against theoretical calculations for the last example.

  3. Características histomorfométricas do intestino de juvenis de tambaqui após uso de probiótico na dieta e durante transporte

    Directory of Open Access Journals (Sweden)

    Celma M. Ferreira

    2014-12-01

    Full Text Available Avaliou-se neste trabalho as características histomorfométricas da mucosa intestinal de tambaqui após uso de probiótico a base de Bacillus spp., veiculado na ração e dissolvido na água durante transporte. Foi utilizado um delineamento inteiramente casualizado com três tratamentos e sete repetições, sendo comparado um tratamento controle (peixes alimentados com ração comercial sem probiótico a dois outros tratamentos envolvendo o uso de probiótico comercial, veiculado na ração ou na água de transporte. Um total de 510 juvenis de tambaqui (peso e comprimento total médio inicial de 83,26±28,14g e 17,39±1,90cm, respectivamente foi distribuído em três tanques de alvenaria. Durante 60 dias, os peixes de dois tanques foram alimentados com ração comercial e os do terceiro tanque com ração comercial suplementada com probiótico. Após este período, os peixes de cada tanque foram divididos em sacos plásticos e transportados por 4 horas de acordo com os seguintes tratamentos: T1 = alimentação com ração comercial (controle; T2 = alimentação com ração comercial e probiótico adicionado na água durante o transporte (20mg/L; T3 = alimentação com ração comercial suplementada com probiótico (1,0g/kg de ração. Antes do transporte (basal, 24 e 96 horas após o transporte, oito peixes de cada tratamento foram submetidos a eutanásia e o intestino retirado para pesagem e mensuração do comprimento para estabelecimento da relação comprimento corporal/ intestino. Em seguida, foi realizada coleta da porção anterior e posterior do intestino, para avaliação das características morfo-histológicas da mucosa intestinal. O uso de probiótico durante o transporte não afetou o peso do intestino de juvenis de tambaqui. O comprimento do intestino do tambaqui não foi alterado pelo tratamento, sendo observada somente relação linear entre o comprimento intestinal e o comprimento corporal (CC dos peixes. A suplementação com

  4. Identification of transporters associated with Etoposide sensitivity of stomach cancer cell lines and methotrexate sensitivity of breast cancer cell lines by quantitative targeted absolute proteomics.

    Science.gov (United States)

    Obuchi, Wataru; Ohtsuki, Sumio; Uchida, Yasuo; Ohmine, Ken; Yamori, Takao; Terasaki, Tetsuya

    2013-02-01

    Membrane transporter proteins may influence the sensitivity of cancer cells to anticancer drugs that can be recognized as substrates. The purpose of this study was to identify proteins that play a key role in the drug sensitivity of stomach and breast cancer cell lines by measuring the absolute protein expression levels of multiple transporters and other membrane proteins and examining their correlation to drug sensitivity. Absolute protein expression levels of 90 membrane proteins were examined by quantitative targeted absolute proteomics using liquid chromatography-linked tandem mass spectrometry. Among them, 11 and 14 membrane proteins, including transporters, were present in quantifiable amounts in membrane fraction of stomach cancer and breast cancer cell lines, respectively. In stomach cancer cell lines, the protein expression level of multidrug resistance-associated protein 1 (MRP1) was inversely correlated with etoposide sensitivity. MK571, an MRP inhibitor, increased both the cell-to-medium ratio of etoposide and the etoposide sensitivity of MRP1-expressing stomach cancer cell lines. In breast cancer cell lines, the protein expression level of reduced folate carrier 1 (RFC1) was directly correlated with methotrexate (MTX) sensitivity. Initial uptake rate and steady-state cell-to-medium ratio of [(3)H]MTX were correlated with both RFC1 expression level and MTX sensitivity. These results suggest that MRP1 modulates the etoposide sensitivity of stomach cancer cell lines and RFC1 modulates the MTX sensitivity of breast cancer cell lines. Our results indicate that absolute quantification of multiple membrane proteins could be a useful strategy for identification of candidate proteins involved in drug sensitivity.

  5. APS Science 2006.

    Energy Technology Data Exchange (ETDEWEB)

    Gibson, J. M.; Fenner, R. B.; Long, G.; Borland, M.; Decker, G.

    2007-05-24

    In my five years as the Director of the Advanced Photon Source (APS), I have been fortunate to see major growth in the scientific impact from the APS. This year I am particularly enthusiastic about prospects for our longer-term future. Every scientific instrument must remain at the cutting edge to flourish. Our plans for the next generation of APS--an APS upgrade--got seriously in gear this year with strong encouragement from our users and sponsors. The most promising avenue that has emerged is the energy-recovery linac (ERL) (see article on page xx), for which we are beginning serious R&D. The ERL{at}APS would offer revolutionary performance, especially for x-ray imaging and ultrafast science, while not seriously disrupting the existing user base. I am very proud of our accelerator physics and engineering staff, who not only keep the current APS at the forefront, but were able to greatly impress our international Machine Advisory Committee with the quality of their work on the possible upgrade option (see page xx). As we prepare for long-term major upgrades, our plans to develop and optimize all the sectors at APS in the near future are advancing. Several new beamlines saw first light this year, including a dedicated powder diffraction beamline (11-BM), two instruments for inelastic x-ray scattering at sector 30, and the Center for Nanoscale Materials (CNM) Nanoprobe beamline at sector 26. Our partnership in the first x-ray free-electron laser (LCLS) to be built at Stanford contributes to revolutionary growth in ultrafast science (see page xx), and we are developing a pulse chirping scheme to get ps pulses at sector 7 of the APS within a year or so. In this report, you will find selected highlights of scientific research at the APS from calendar year 2006. The highlighted work covers diverse disciplines, from fundamental to applied science. In the article on page xx you can see the direct impact of APS research on technology. Several new products have emerged from

  6. AP statistics crash course

    CERN Document Server

    D'Alessio, Michael

    2012-01-01

    AP Statistics Crash Course - Gets You a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. AP Statistics Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know Crash Course is based on an in-depth analysis of the AP Statistics course description outline and actual Advanced Placement test questions. It covers only the information tested on the exam, so you can make the most of your valuable study time. Our easy-to-read format covers: exploring da

  7. Numerical Analysis of Impurity Transport along Magnetic Field Lines in Tokamak Scrape-Off Layer

    Science.gov (United States)

    Chung, Tae Kyun; Hong, Sang Hee

    1996-11-01

    A flow of impurity ions along the magnetic field lines in tokamak SOL (scrape-off layer) is investigated by a one-dimensional numerical analysis. The background values of SOL plasma, fields such as density, velocity, temperature and electric field, are obtained from an edge plasma transport code EDGETRAN(A 2-D edge plasma transport code with limited and diverted tokamak configurations developed by Dr. Kihak Im at Seoul National University). Impurity ions are generated by ionizations of neutral atoms in tokamak. The density profile of neutral atoms is given at an initial state. Impurity ions in a single-charged state develop into multi-charged states by ionization and recombination reactions. Impurity productions at the divertor target plate by sputterings are also considered as main source terms. As a numerics, FDM(Finite Difference Method) is employed. The Neumann condition on impurity density and the Dirchlet condition on impurity velocity are provided for their boundary conditions at a symmetry plane of the layer. No strict boundary condition on the target plate is given except an external source prescribed by sputtering rates.

  8. Orbit error correction on the high energy beam transport line at the KHIMA accelerator system

    Science.gov (United States)

    Park, Chawon; Yim, Heejoong; Hahn, Garam; An, Dong Hyun

    2016-09-01

    For the purpose of treatment of various cancers and medical research, a synchrotron based medical machine has been developed under the Korea Heavy Ion Medical Accelerator (KHIMA) project and is scheduled for use to treat patient at the beginning of 2018. The KHIMA synchrotron is designed to accelerate and extract carbon ion (proton) beams with various energies from 110 to 430 MeV/u (60 to 230 MeV). Studies on the lattice design and beam optics for the High Energy Beam Transport (HEBT) line at the KHIMA accelerator system have been carried out using the WinAgile and the MAD-X codes. Because magnetic field errors and misalignments introduce deviations from the design parameters, these error sources should be treated explicitly, and the sensitivity of the machine's lattice to different individual error sources should be considered. Various types of errors, both static and dynamic, have been taken into account and have been consequentially corrected with a dedicated correction algorithm by using the MAD-X program. Based on the error analysis, the optimized correction setup is decided, and the specifications for the correcting magnets of the HEBT lines are determined.

  9. APS Science 2009.

    Energy Technology Data Exchange (ETDEWEB)

    Gibson, J. M; Mills, D. M.; Gerig, R.

    2010-05-01

    It is my pleasure to introduce the 2009 annual report of the Advanced Photon Source. This was a very good year for us. We operated with high reliability and availability, despite growing problems with obsolete systems, and our users produced a record output of publications. The number of user experiments increased by 14% from 2008 to more than 3600. We congratulate the recipients of the 2009 Nobel Prize in Chemistry-Venkatraman Ramakrishnan (Cambridge Institute for Medical Research), Thomas Steitz (Yale University), and Ada Yonath (Weizmann Institute) - who did a substantial amount of this work at APS beamlines. Thanks to the efforts of our users and staff, and the ongoing counsel of the APS Scientific Advisory Committee, we made major progress in advancing our planning for the upgrade of the APS (APS-U), producing a proposal that was positively reviewed. We hope to get formal approval in 2010 to begin the upgrade. With advocacy from our users and the support of our sponsor, the Office of Basic Energy Sciences in the Department of Energy (DOE) Office of Science, our operating budgets have grown to the level needed to more adequately staff our beamlines. We were also extremely fortunate to have received $7.9 M in American Recovery and Reinvestment Act ('stimulus') funding to acquire new detectors and improve several of our beamlines. The success of the new Linac Coherent Light Source at Stanford, the world's first x-ray free-electron laser, made us particularly proud since the undulators were designed and built by the APS. Among other highlights, we note that more than one-quarter of the 46 Energy Frontier Research Centers, funded competitively across the U.S. in 2009 by the DOE, included the Advanced Photon Source in their proposed work, which shows that synchrotron radiation, and the APS in particular, are central to energy research. While APS research covers everything from fundamental to applied science (reflected by the highlights in this report

  10. Parametric study of transport beam lines for electron beams accelerated by laser-plasma interaction

    Science.gov (United States)

    Scisciò, M.; Lancia, L.; Migliorati, M.; Mostacci, A.; Palumbo, L.; Papaphilippou, Y.; Antici, P.

    2016-03-01

    In the last decade, laser-plasma acceleration of high-energy electrons has attracted strong attention in different fields. Electrons with maximum energies in the GeV range can be laser-accelerated within a few cm using multi-hundreds terawatt (TW) lasers, yielding to very high beam currents at the source (electron bunches with up to tens-hundreds of pC in a few fs). While initially the challenge was to increase the maximum achievable electron energy, today strong effort is put in the control and usability of these laser-generated beams that still lack of some features in order to be used for applications where currently conventional, radio-frequency (RF) based, electron beam lines represent the most common and efficient solution. Several improvements have been suggested for this purpose, some of them acting directly on the plasma source, some using beam shaping tools located downstream. Concerning the latter, several studies have suggested the use of conventional accelerator magnetic devices (such as quadrupoles and solenoids) as an easy implementable solution when the laser-plasma accelerated beam requires optimization. In this paper, we report on a parametric study related to the transport of electron beams accelerated by laser-plasma interaction, using conventional accelerator elements and tools. We focus on both, high energy electron beams in the GeV range, as produced on petawatt (PW) class laser systems, and on lower energy electron beams in the hundreds of MeV range, as nowadays routinely obtained on commercially available multi-hundred TW laser systems. For both scenarios, our study allows understanding what are the crucial parameters that enable laser-plasma accelerators to compete with conventional ones and allow for a beam transport. We show that suitable working points require a tradeoff-combination between low beam divergence and narrow energy spread.

  11. MEMBRANE-TRANSPORT OF METHOTREXATE IN A SQUAMOUS CARCINOMA CELL-LINE ADAPTED TO LOW FOLATE CONCENTRATIONS

    NARCIS (Netherlands)

    van der Laan, B.F.A.M.; Jansen, G; VANGESTEL, JA; SCHORNAGEL, JH; HORDIJK, GJ

    1991-01-01

    Membrane transport characteristics of the folate analogue methotrexate (MTX) were studied in a human squamous carcinoma cell line of the head and neck (HNSCC) adapted to grow in tissue culture media with nanomolar reduced folate concentrations (SCC-11B-LF), as compared to SCC-11B cells grown in stan

  12. Orbit Error Correction on the High Energy Beam Transport Line at the KHIMA Accelerator System

    CERN Document Server

    Park, Chawon; Hahn, Garam; An, Dong Hyun

    2016-01-01

    For the purpose of treatment of various cancer and medical research, the synchrotron based medical machine under the Korea Heavy Ion Medical Accelerator (KHIMA) project have been conducted and is going to treat the patient at the beginning of 2018. The KHIMA synchrotron is designed to accelerate and extract the carbon ion (proton) beam with various energy range, 110 up to 430 MeV/u (60 up to 230 MeV). A lattice design and beam optics studies for the High Energy Beam Transport (HEBT) line at the KHIMA accelerator system have been carried out with WinAgile and the MAD-X codes. Because the magnetic eld errors and the mis-alignments introduce to the deviations from the design parameters, these error sources should be treated explicitly and the sensitivity of the machine's lattice to di erent individual error sources is considered. Various types of errors which are static and dynamic one have been taken into account and have been consequentially corrected with a dedicated correction algorithm by using the MAD-X pr...

  13. Improved design of proton source and low energy beam transport line for European Spallation Source

    Energy Technology Data Exchange (ETDEWEB)

    Neri, L., E-mail: neri@lns.infn.it; Celona, L.; Gammino, S.; Mascali, D.; Castro, G.; Ciavola, G. [Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, Via S. Sofia 62, 95123 Catania (Italy); Torrisi, G. [Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, Via S. Sofia 62, 95123 Catania (Italy); Dipartimento di Ingegneria dell’Informazione, delle Infrastrutture e dell’Energia Sostenibile, Università Mediterranea di Reggio Calabria, Via Graziella, 89122 Reggio Calabria (Italy); Cheymol, B.; Ponton, A. [European Spallation Source ESS AB, Lund (Sweden); Galatà, A. [Laboratori Nazionali di Legnaro, Istituto Nazionale di Fisica Nucleare, Viale dell' università 2, 35020 Legnaro (Italy); Patti, G. [Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, Via S. Sofia 62, 95123 Catania (Italy); Laboratori Nazionali di Legnaro, Istituto Nazionale di Fisica Nucleare, Viale dell' università 2, 35020 Legnaro (Italy); Gozzo, A.; Lega, L. [Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, Via S. Sofia 62, 95123 Catania (Italy); Dipartimento di Ingegneria Informatica e delle Telecomunicazioni, Università degli Studi di Catania, Viale Andrea Doria 6, 95123 Catania (Italy)

    2014-02-15

    The design update of the European Spallation Source (ESS) accelerator is almost complete and the construction of the prototype of the microwave discharge ion source able to provide a proton beam current larger than 70 mA to the 3.6 MeV Radio Frequency Quadrupole (RFQ) started. The source named PS-ESS (Proton Source for ESS) was designed with a flexible magnetic system and an extraction system able to merge conservative solutions with significant advances. The ESS injector has taken advantage of recent theoretical updates and new plasma diagnostics tools developed at INFN-LNS (Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare). The design strategy considers the PS-ESS and the low energy beam transport line as a whole, where the proton beam behaves like an almost neutralized non-thermalized plasma. Innovative solutions have been used as hereinafter described. Thermo-mechanical optimization has been performed to withstand the chopped beam and the misaligned focused beam over the RFQ input collimator; the results are reported here.

  14. Improved design of proton source and low energy beam transport line for European Spallation Source

    Science.gov (United States)

    Neri, L.; Celona, L.; Gammino, S.; Mascali, D.; Castro, G.; Torrisi, G.; Cheymol, B.; Ponton, A.; Galatà, A.; Patti, G.; Gozzo, A.; Lega, L.; Ciavola, G.

    2014-02-01

    The design update of the European Spallation Source (ESS) accelerator is almost complete and the construction of the prototype of the microwave discharge ion source able to provide a proton beam current larger than 70 mA to the 3.6 MeV Radio Frequency Quadrupole (RFQ) started. The source named PS-ESS (Proton Source for ESS) was designed with a flexible magnetic system and an extraction system able to merge conservative solutions with significant advances. The ESS injector has taken advantage of recent theoretical updates and new plasma diagnostics tools developed at INFN-LNS (Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare). The design strategy considers the PS-ESS and the low energy beam transport line as a whole, where the proton beam behaves like an almost neutralized non-thermalized plasma. Innovative solutions have been used as hereinafter described. Thermo-mechanical optimization has been performed to withstand the chopped beam and the misaligned focused beam over the RFQ input collimator; the results are reported here.

  15. Beam Diagnostics Instrumentation for the High Energy Beam Transport Line of I.P.H.I.

    CERN Document Server

    Ausset, P; Coacolo, J L; Lesrel, J; Maymon, J N; Olivier, A; Rouviere, N; Solal-Cohen, M; Vatrinet, L; Yaniche, J F

    2005-01-01

    I.P.H.I. is a High Intensity Proton Injector under construction at Saclay (C.N.R.S/ I.N.2P.3; C.E.A. / D.A.P.N.I.A and C.E.R.N. collaboration). An E.C.R. produces a 100 keV, 100 mA C.W. proton beam which will be accelerated at 3 MeV by a 4 vanes R.F.Q. operating at 352.2 MHz. Finally, a High Energy Beam Transport Line (H.E.B.T.) will deliver the beam to a beam stopper and will be equipped with appropriate beam diagnostics to carry intensity; centroïd beam transverse position, transverse beam profiles, beam energy and energy spread measurements for the commissioning of I.P.H.I. These beam diagnostics will operate under both pulsed and C.W. operation. Transverse beam profile measurements will be acquired under low and high duty factor pulsed beam operation using a slow wire scanner and a C.C.D. camera to image the beam-induced fluorescence. The beam instrumentation of the H.E.B.T. is reviewed and preliminary obtained transverse profile measurements at 100 keV are described.

  16. Effects of line defects on spin-dependent electronic transport of zigzag MoS2 nanoribbons

    Directory of Open Access Journals (Sweden)

    Xin-Mei Li

    2016-01-01

    Full Text Available The nonlinear spin-dependent transport properties in zigzag molybdenum-disulfide nanoribbons (ZMNRs with line defects are investigated systematically using nonequilibrium Green’s function method combined with density functional theory. The results show that the line defects can enhance the electronic transfer ability of ZMNRs. The types and locations of the line defects are found critical in determining the spin polarization and the current-voltage (I-V characteristics of the line defected ZMNRs. For the same defect type, the total currents of the ribbons with the line defects in the centers are lager than those on the edges. And for the same location, the total currents of the systems with the sulfur (S line defect are larger than the according systems with the molybdenum (Mo line defect. All the considered systems present magnetism properties. And in the S line defected systems, the spin reversal behaviors can be observed. In both the spin-up and spin-down states of the Mo line defected systems, there are obvious negative differential resistance behaviors. The mechanisms are proposed for these phenomena.

  17. Effects of line defects on spin-dependent electronic transport of zigzag MoS{sub 2} nanoribbons

    Energy Technology Data Exchange (ETDEWEB)

    Li, Xin-Mei; Yang, Kai-Wei; Zhang, Dan; Ding, Jia-Feng; Xu, Hui, E-mail: xuhui@csu.edu.cn [Institute of Super-microstructure and Ultrafast Process in Advanced Materials & Hunan Key Laboratory for Super-microstructure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha 410083 (China); Long, Meng-Qiu, E-mail: mqlong@csu.edu.cn [Institute of Super-microstructure and Ultrafast Process in Advanced Materials & Hunan Key Laboratory for Super-microstructure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha 410083 (China); Department of Physics and Materials Science, City University of Hong Kong, Hong Kong (China); Cui, Li-Ling [Institute of Super-microstructure and Ultrafast Process in Advanced Materials & Hunan Key Laboratory for Super-microstructure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha 410083 (China); School of Science, Hunan University of Technology, Zhuzhou 412007 (China)

    2016-01-15

    The nonlinear spin-dependent transport properties in zigzag molybdenum-disulfide nanoribbons (ZMNRs) with line defects are investigated systematically using nonequilibrium Green’s function method combined with density functional theory. The results show that the line defects can enhance the electronic transfer ability of ZMNRs. The types and locations of the line defects are found critical in determining the spin polarization and the current-voltage (I-V) characteristics of the line defected ZMNRs. For the same defect type, the total currents of the ribbons with the line defects in the centers are lager than those on the edges. And for the same location, the total currents of the systems with the sulfur (S) line defect are larger than the according systems with the molybdenum (Mo) line defect. All the considered systems present magnetism properties. And in the S line defected systems, the spin reversal behaviors can be observed. In both the spin-up and spin-down states of the Mo line defected systems, there are obvious negative differential resistance behaviors. The mechanisms are proposed for these phenomena.

  18. Characterization of a Chinese hamster-human hybrid cell line with increased system L amino acid transport activity.

    Science.gov (United States)

    Lobaton, C D; Moreno, A; Oxender, D L

    1984-03-01

    We have studied leucine transport in several Chinese hamster-human hybrid cell lines obtained by fusion of a temperature-sensitive line of Chinese hamster ovary cells, ts025C1, and normal human leukocytes. A hybrid cell line exhibiting a twofold increase in L-leucine uptake over that in the parental cell line was found. This hybrid cell line, 158CnpT-1, was temperature resistant, whereas the parental Chinese hamster ovary mutant, ts025C1, contained a temperature-sensitive leucyl-tRNA synthetase mutation. An examination of the different amino acid transport systems in this hybrid cell line revealed a specific increase of system L activity with no significant changes in systems A and ASC. The Vmax for L-leucine uptake exhibited by the hybrid 158CnpT-1 was twice that in the CHO parental mutant, ts025C1. Cytogenetic analysis showed that the hybrid 158CnpT-1 contains four complete human chromosomes (numbers 4, 5, 10, and 21) and three interspecific chromosomal translocations in a total complement of 34 chromosomes. Biochemical and cytogenetic analysis of segregant clones obtained from hybrid 158CnpT-1 showed that the primary temperature resistance and high system L transport phenotypes can be segregated from this hybrid independently. The loss of the primary temperature resistance was associated with the loss of the human chromosome 5, as previously reported by other laboratories, whereas the loss of the high leucine transport phenotype, which is associated with a lesser degree of temperature resistance, was correlated with the loss of human chromosome 20.

  19. The Value of Information in Container Transport: Leveraging the Triple Bottom Line

    NARCIS (Netherlands)

    R.A. Zuidwijk (Rob); A.W. Veenstra (Albert)

    2010-01-01

    textabstractPlanning the transport of maritime containers from the sea port to final destinations while using multiple transport modes is challenged by uncertainties regarding the time the container is released for further transport or the transit time from the port to its final destination. This pa

  20. Bending of protonema cells in a plastid glycolate/glycerate transporter knockout line of Physcomitrella patens.

    Directory of Open Access Journals (Sweden)

    Jin Nakahara

    Full Text Available Arabidopsis LrgB (synonym PLGG1 is a plastid glycolate/glycerate transporter associated with recycling of 2-phosphoglycolate generated via the oxygenase activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO. We isolated two homologous genes (PpLrgB1 and B2 from the moss Physcomitrella patens. Phylogenetic tree analysis showed that PpLrgB1 was monophyletic with LrgB proteins of land plants, whereas PpLrgB2 was divergent from the green plant lineage. Experiments with PpLrgB-GFP fusion proteins suggested that both PpLrgB1 and B2 proteins were located in chloroplasts. We generated PpLrgB single (∆B1 and ∆B2 and double (∆B1/∆B2-knockout lines using gene targeting of P. patens. The ∆B1 plants showed decreases in growth and photosynthetic activity, and their protonema cells were bent and accumulated glycolate. However, because ∆B2 and ∆B1/∆B2 plants showed no obvious phenotypic change relative to the wild-type or ∆B1 plants, respectively, the function of PpLrgB2 remains unclear. Arabidopsis LrgB could complement the ∆B1 phenotype, suggesting that the function of PpLrgB1 is the same as that of AtLrgB. When ∆B1 was grown under high-CO2 conditions, all novel phenotypes were suppressed. Moreover, protonema cells of wild-type plants exhibited a bending phenotype when cultured on media containing glycolate or glycerate, suggesting that accumulation of photorespiratory metabolites caused P. patens cells to bend.

  1. Lattice design and beam dynamics studies of the high energy beam transport line in the RAON heavy ion accelerator

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Hyunchang, E-mail: hcjin@ibs.re.kr; Jang, Ji-Ho; Jang, Hyojae; Jeon, Dong-O

    2015-12-01

    In RAON heavy ion accelerator, beams generated by superconducting electron cyclotron resonance ion source (ECR-IS) or Isotope Separation On-Line (ISOL) system are accelerated by lower energy superconducting linac and high energy superconducting linac. The accelerated beams are used in the high energy experimental hall which includes bio-medical and muon-SR facilities, after passing through the high energy beam transport lines. At the targets of those two facilities, the stable and small beams meeting the requirements rigorously are required in the transverse plane. Therefore the beams must be safely sent to the targets and simultaneously satisfy the two requirements, the achromatic condition and the mid-plane symmetric condition, of the targets. For this reason, the lattice design of the high energy beam transport lines in which the long deflecting sections are included is considered as a significant issue in the RAON accelerator. In this paper, we will describe the calculated beam optics satisfying the conditions and present the result of particle tracking simulations with the designed lattice of the high energy beam transport lines in the RAON accelerator. Also, the orbit distortion caused by the machine imperfections and the orbit correction with correctors will be discussed.

  2. 1. 5 GeV/c multiturn shaving extraction and its transport line for the Brookhaven AGS

    Energy Technology Data Exchange (ETDEWEB)

    Weng, W.T.; Blumberg, L.N.; Gill, E.; Soukas, A.; Witkover, R.L.; Egleman, E.; LoSecco, J.; Sulak, L.

    1979-01-01

    A system for fast shaving extraction at 1.5 GeV/c is implemented to extract the circulating beam in five turns. A numerical simulation is first carried out to determine the emittance and the rf structure of the extracted beam. This is followed by several machine study sessions which establish the optimal extraction configuration, confirm the emittance, and modify the transport line for low energy beam. Finally, a one-week run for the Neutrino Oscillation experiment demonstrates that the system is very stable and capable of delivering 7.5 x 10/sup 12/ p/sec with 70% extraction efficiency and 95% transport efficiency.

  3. Stress responses of juvenile matrinxã (Brycon amazonicus after transport in a closed system under different loading densities Respostas de estresse de matrinxã (Brycon amazonicus juvenil após transporte em sistema fechado sob diferentes densidades de carga

    Directory of Open Access Journals (Sweden)

    Janessa Sampaio de Abreu

    2008-08-01

    Full Text Available Optimum loading density for the transport of matrinxã juveniles was investigated in a closed system with plastic bags. Transport was conducted for 4 h with fish (23.5± 0.4g and 11.6 (0.08cm starved for 24h at loading densities of 83g L-1 (D1, 125g L-1 (D2, 168g L-1 (D3 and 206g L-1 (D4. Fish were sampled before transport (BT, after transport (AT and 24h AT. The water quality was monitored before capturing fish in depuration tanks, after transport in plastic bags and in recovery tanks. Water oxygen decreased to values below 4mg L-1 in D2, D3 and D4, temperature was around 32°C, pH 6.5-6.78, total ammonia 1.09-1.7mg L-1, un-ionized ammonia 3.58-9.33x10³mg L-1 and alkalinity 134-165mg CaCO3 L-1. Blood cortisol and glucose concentrations increased in fish of all densities AT and recovered the BT levels 24h after arrival. Osmolality did not change AT but increased 24h AT in fish of all densities whereas plasma chloride decreased inversely to the loading densities after the procedure. Hematocrit decreased 24h AT in fish of all densities but no differences were verified in the number of erythrocytes. No mortality was registered in any treatment during the next week after transport. Matrinxã demonstrated to be a crowding tolerant-species in transport operations besides tolerating low levels of oxygen in the water.Neste estudo, foram investigadas as densidades de carga adequadas para transporte de matrinxãs juvenis em sistema fechado com sacos plásticos. O transporte de 4h foi feito com peixes (23,5±0,4g; 11,6 (0,08cm em jejum por 24h, em densidades de 83g L-1 (D1, 125g L-1 (D2, 168g L-1 (D3 e 206g L-1 (D4. Os peixes foram amostrados antes do transporte (AT, logo após o transporte (chegada (DT e 24h depois. A qualidade da água foi monitorada antes da captura dos peixes nos tanques de depuração, após o transporte nos sacos plásticos e nos tanques de recuperação. O oxigênio da água diminuiu para valores inferiores a 4mg L-1 em D2, D3 e D4

  4. Upgrade of the beam transport lines and the beam-abort system and development of a tune compensator in KEKB

    Science.gov (United States)

    Iida, Naoko; Kikuchi, Mitsuo; Mimashi, Toshihiro; Nakayama, Hisayoshi; Sakamoto, Yutaka; Satoh, Kotaro; Takasaki, Seiji; Tawada, Masafumi

    2013-03-01

    The KEKB collider achieved a maximum peak luminosity of 2.1×1034 cm-2 s-1 and an integrated luminosity of 1 ab-1 in its ten-year operation. Behind these glorious records there have been uncountable improvements in every subsystem. This paper describes the improvements in the beam transport line, injection kickers, septum magnets, the beam-abort system, and a newly developed pulsed-quadrupole system in detail.

  5. Paracellular transport through healthy and cystic fibrosis bronchial epithelial cell lines--do we have a proper model?

    Directory of Open Access Journals (Sweden)

    Natalia Molenda

    Full Text Available It has been reported recently that the cystic fibrosis transmembrane conductance regulator (CFTR besides transcellular chloride transport, also controls the paracellular permeability of bronchial epithelium. The aim of this study was to test whether overexpressing wtCFTR solely regulates paracellular permeability of cell monolayers. To answer this question we used a CFBE41o- cell line transfected with wtCFTR or mutant F508del-CFTR and compered them with parental line and healthy 16HBE14o- cells. Transepithelial electrical resistance (TER and paracellular fluorescein flux were measured under control and CFTR-stimulating conditions. CFTR stimulation significant decreased TER in 16HBE14o- and also in CFBE41o- cells transfected with wtCFTR. In contrast, TER increased upon stimulation in CFBE41o- cells and CFBE41o- cells transfected with F508del-CFTR. Under non-stimulated conditions, all four cell lines had similar paracellular fluorescein flux. Stimulation increased only the paracellular permeability of the 16HBE14o- cell monolayers. We observed that 16HBE14o- cells were significantly smaller and showed a different structure of cell-cell contacts than CFBE41o- and its overexpressing clones. Consequently, 16HBE14o- cells have about 80% more cell-cell contacts through which electrical current and solutes can leak. Also tight junction protein composition is different in 'healthy' 16HBE14o- cells compared to 'cystic fibrosis' CFBE41o- cells. We found that claudin-3 expression was considerably stronger in 16HBE14o- cells than in the three CFBE41o- cell clones and thus independent of the presence of functional CFTR. Together, CFBE41o- cell line transfection with wtCFTR modifies transcellular conductance, but not the paracellular permeability. We conclude that CFTR overexpression is not sufficient to fully reconstitute transport in CF bronchial epithelium. Hence, it is not recommended to use those cell lines to study CFTR-dependent epithelial transport.

  6. Enhancement of glucose transport by selected plant foods in muscle cell line L6.

    Science.gov (United States)

    Noipha, K; Ratanachaiyavong, S; Ninla-Aesong, P

    2010-08-01

    Glucose uptake activity of 11 plant foods was assessed in L6 myotubes. Among them onion and ginger showed potent enhancement of glucose transport. This effect required new protein synthesis of glucose transporters. In addition, onion-induced glucose uptake in L6 myotubes was mediated through activation of phosphoinositide 3-kinase.

  7. Postendocytic sorting of constitutively internalized dopamine transporter in cell lines and dopaminergic neurons

    DEFF Research Database (Denmark)

    Eriksen, Jacob; Bjørn-Yoshimoto, Walden Emil; Jørgensen, Trine Nygaard;

    2010-01-01

    The dopamine transporter (DAT) mediates reuptake of released dopamine and is the target for psychostimulants, such as cocaine and amphetamine. DAT undergoes marked constitutive endocytosis, but little is known about the fate and sorting of the endocytosed transporter. To study DAT sorting in cell...

  8. Transport of poly(acrylic acid) coated 2-line ferrihydrite nanoparticles in saturated aquifer sediments for environmental remediation

    Science.gov (United States)

    Xiang, Aishuang; Zhou, Sheng; Koel, Bruce E.; Jaffé, Peter R.

    2014-04-01

    Groundwater remediation using iron oxide and zero-valent iron nanoparticles (NPs) can be effective, but is limited in many applications due to the NP strong retention in groundwater-saturated porous media after injection, the passivation of the porous surface, and the high cost of nanomaterials versus macro scale iron. In this study, we investigated transport of bare and polymer-coated 2-line ferrihydrite NPs (30-300 nm) in saturated aquifer sediments. The influence of poly(acrylic acid) (PAA) polymer coatings was studied on the colloidal stability and transport in sediments packed column tests simulating groundwater flow in saturated sediments. In addition, the influence of calcium cations was investigated by transport measurements using sediments with calcium concentrations in the aqueous phase ranging from 0.5 (typical for most sediments) to 2 mM. Measurements were also made of zeta potential, hydrodynamic diameter, polymer adsorption and desorption properties, and bio-availability of PAA-coated NPs. We found that NP transport through the saturated aquifer sediments was improved by PAA coating and that the transport properties could be tuned by adjusting the polymer concentration. We further discovered that PAA coatings enhanced NP transport, compared to bare NPs, in all calcium-containing experiments tested, however, the presence of calcium always exhibited a negative effect on NP transport. In tests of bioavailability, the iron reduction rate of the coated and bare NPs by Geobacter sulfurreducens was the same, which shows that the PAA coating does not significantly reduce NP Fe(III) bioavailability. Our results demonstrate that much improved transport of iron oxide NP can be achieved in saturated aquifer sediments by introducing negatively charged polyelectrolytes and optimizing polymer concentrations, and furthermore, these coated NPs retain their bioavailability that is needed for applications in bio-environmental remediation.

  9. Influence of a hot and humid environment on thermal transport across the interface between a Ag thin-film line and a substrate

    Science.gov (United States)

    Li, Yuan; Noguchi, Kyohei; Saka, Masumi

    2016-04-01

    To evaluate the reliability of Ag thin-film lines for a wide range of applications in electronic devices, knowledge of the thermal transport across the interface between the line and the underlying substrate is of great importance. This is because such thermal transport significantly affects the temperature distribution in the line, the electrical performance of the line and the service life of the device the line is installed on. In this work, we examine the influence of a hot and humid environment on the thermal transport across the interface between a Ag thin-film line and a substrate. By performing a series of current-stressing experiments using the four-point probe method at atmospheric conditions (296 K and 30 RH%) on a Ag thin-film line for different durations of exposure to a hot and humid environment (323 K and 90 RH%), the electrical resistivity was found to increase with the exposure duration. Such an increase is believed to be the result of a decrease in the interfacial thermal conductance, which indicates less thermal transport from the line to the substrate. Moreover, by observing the surface morphology changes in the line and conducting a one-dimensional electro-thermal analysis, such variations can be attributed to the generation and growth of voids within the line, which hinder heat transfer from the line to the substrate through the interface.

  10. The APS ceramic chambers

    Energy Technology Data Exchange (ETDEWEB)

    Milton, S.; Warner, D.

    1994-07-01

    Ceramics chambers are used in the Advanced Photon Source (APS) machines at the locations of the pulsed kicker and bumper magnets. The ceramic will be coated internally with a resistive paste. The resistance is chosen to allow the low frequency pulsed magnet field to penetrate but not the high frequency components of the circulating beam. Another design goal was to keep the power density experienced by the resistive coating to a minimum. These ceramics, their associated hardware, the coating process, and our recent experiences with them are described.

  11. Transepithelial transport of putrescine across monolayers of the human intestinal epithelial cell line, Caco-2

    Institute of Scientific and Technical Information of China (English)

    Vladan Milovic; Lyudmila Turchanowa; Jurgen Stein; Wolfgang F. Caspary

    2001-01-01

    AIM To study the transepithelial transport characteristics of the polyamine putrescine in human intestinal Caco-2 cell monolayers to elucidate the mechanisms of the putrescine intestinal absorption.METHODS The transepithelial transport and the cellular accumulation of putrescine was measured using Caco 2 cell monolayers grown on permeable filters.RESULTS Transepithelial transport of putrescine in physiological concentrations (>0.5 mM)from the apical to basolateral side was linear. Intracellular accumulation of putrescine was higher in confluent than in fully differentiated Caco-2 cells, but still negligible (less than 0.5%) of the overall transport across the monolayers in apical-to-basolateral direction. EGF enhanced putrescine accumulation in Caco-2 cells by four-fold, as well as putrescine conversion to spermidine and spermine by enhancing the activity of Sadenosylmethionine decarboxylase. However,EGF did not have any significant influence on putrescine flux across the Caco-2 cell monolayers. Excretion of putrescine from Caco-2cells into the basolateral medium did not exceed 50 picomoles, while putrescine passive flux from the apical to the basolateral chamber,contributed hundreds of micromoles polyamines to the basolateral chamber.CONCLUSION Transepithelial transport of putrescine across Caco-2 cell monolayers occurs in passive diffusion, and is not influenced when epithelial cells are stimulated to proliferate by a potent mitogen such as EGF.

  12. Epidermal growth factor inhibits glycylsarcosine transport and hPepT1 expression in a human intestinal cell line

    DEFF Research Database (Denmark)

    Nielsen, C U; Amstrup, J; Steffansen, B;

    2001-01-01

    of the fluxes showed that maximal flux (V(max)) of transepithelial transport decreased from 3.00 +/- 0.17 nmol x cm(-2) x min(-1) in control cells to 0.50 +/- 0.07 nmol x cm(-2) x min(-1) in cells treated with 5 ng/ml EGF (n = 6, P Michaelis-Menten constant (K(m)) was 2.71 +/- 0.31 mM (n......The human intestinal cell line Caco-2 was used as a model system to study the effects of epidermal growth factor (EGF) on peptide transport. EGF decreased apical-to-basolateral fluxes of [(14)C]glycylsarcosine ([(14)C]Gly-Sar) up to 50.2 +/- 3.6% (n = 6) of control values. Kinetic analysis...

  13. An Agent-based Transportation Route Planning Method for Led Fabricating Line and Its Evaluation Using Lagrangian Relaxation

    Science.gov (United States)

    Nishi, Tatsushi; Sotobayashi, Ken; Ando, Masakazu; Konishi, Masami

    In most of Light Emitting Diode (LED) semiconductor fabricating line, AGVs (Automated Guided Vehicles) are used to transport the products between the facilities in order to ensure dust-free operation in the transportation systems. It has been demanded to realize the route planning system which speedily derives a feasible transportation route so that each AGV does not interfere with other AGVs. In this paper, we applied an agent-based optimization method for the route planning problems with multiple AGVs in LED fabricating bay in which the total transportation time is minimized. In the agent-based method, each AGV agent independently searches its candidate of route. The imitated pheromone is effectively used to generate the route which avoids the collision among AGVs. In order to evaluate the performance of the proposed method, the lower bound of the original problem is obtained by using the Lagrangian relaxation technique. The performances of the proposed method are evaluated by using the value of duality gap for a large scale problem. The effectiveness of the agent-based optimization method is demonstrated.

  14. CGM ApS Årsberetning til DANAK

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2003. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, ErhvervsfremmeStyrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler.......Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2003. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, ErhvervsfremmeStyrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler....

  15. Multidrug resistance mediated by ABC transporters in osteosarcoma cell lines: mRNA analysis and functional radiotracer studies

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, Celia Maria Freitas [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Faculty of Medicine, Institute of Biophysics/Biomathematics, IBILI, 3000-354 Coimbra (Portugal)]. E-mail: cgomes@ibili.uc.pt; van Paassen, Heidi [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Romeo, Salvatore [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Welling, Mick M. [Department of Radiology, Section of Nuclear Medicine, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Feitsma, R.I.J. [Department of Radiology, Section of Nuclear Medicine, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Abrunhosa, Antero J. [Faculty of Medicine, Institute of Biophysics/Biomathematics, IBILI, 3000-354 Coimbra (Portugal); Botelho, M. Filomena [Faculty of Medicine, Institute of Biophysics/Biomathematics, IBILI, 3000-354 Coimbra (Portugal); Hogendoorn, Pancras C.W. [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Pauwels, Ernest [Department of Radiology, Section of Nuclear Medicine, Leiden University Medical Center, 2300 RC Leiden (Netherlands); Cleton-Jansen, Anne Marie [Department of Pathology, Leiden University Medical Center, 2300 RC Leiden (Netherlands)

    2006-10-15

    Drug resistance remains a significant impediment to successful chemotherapy and constitutes a major prognostic factor in osteosarcoma (OS) patients. This study was designed to identify the role and prognostic significance of multidrug-resistance (MDR)-related transporters, such as multidrug resistance protein 1 (MDR1), multidrug-resistance-associated protein (MRP1) and breast-cancer-related protein (BCRP), in OS using cationic lipophilic radiotracers. We evaluated the chemosensitivity of four OS cell lines (Saos-2, 143B, MNNG/HOS and U-2OS) to doxorubicin (DOX), cisplatin (CIS) and methotrexate. The expression of MDR-related transporters was analyzed at mRNA level by quantitative polymerase chain reaction and at functional level by {sup 99m}Tc sestamibi and {sup 99m}Tc tetrofosmin. The effectiveness of MDR modulators [cyclosporin A (CsA) and imatinib] on transporter inhibition and on the reversal of resistance was also assessed. MNNG/HOS and U-2OS cells expressing high levels of MDR1 were highly resistant to DOX and showed reduced accumulation and higher efflux for radiotracers. Although MRP1 was uniformly expressed in all cells, only U-2OS was resistant to CIS. CsA restored sensitivity to DOX and CIS, and enhanced the accumulation and efflux half-life of radiotracers in MDR1-expressing cell lines. The chemosensitivity of OS cells to DOX was strongly dependent on mRNA MDR1 expression and could be circumvented by adding CsA. The kinetic parameters of radiotracers correlated with MDR1 expression levels, hence predicting DOX resistance. We concluded that sensitivity to chemotherapy is strongly dependent on the expression of MDR1 transporter and that radiotracer studies could prove clinically useful in predicting chemotherapy response and in evaluating the efficacy of MDR-reversing agents.

  16. Space charge compensation in the Linac4 low energy beam transport line with negative hydrogen ions.

    Science.gov (United States)

    Valerio-Lizarraga, Cristhian A; Lallement, Jean-Baptiste; Leon-Monzon, Ildefonso; Lettry, Jacques; Midttun, Øystein; Scrivens, Richard

    2014-02-01

    The space charge effect of low energy, unbunched ion beams can be compensated by the trapping of ions or electrons into the beam potential. This has been studied for the 45 keV negative hydrogen ion beam in the CERN Linac4 Low Energy Beam Transport using the package IBSimu [T. Kalvas et al., Rev. Sci. Instrum. 81, 02B703 (2010)], which allows the space charge calculation of the particle trajectories. The results of the beam simulations will be compared to emittance measurements of an H(-) beam at the CERN Linac4 3 MeV test stand, where the injection of hydrogen gas directly into the beam transport region has been used to modify the space charge compensation degree.

  17. Space charge compensation in the Linac4 low energy beam transport line with negative hydrogen ions

    Energy Technology Data Exchange (ETDEWEB)

    Valerio-Lizarraga, Cristhian A., E-mail: cristhian.alfonso.valerio.lizarraga@cern.ch [CERN, Geneva (Switzerland); Departamento de Investigación en Física, Universidad de Sonora, Hermosillo (Mexico); Lallement, Jean-Baptiste; Lettry, Jacques; Scrivens, Richard [CERN, Geneva (Switzerland); Leon-Monzon, Ildefonso [Facultad de Ciencias Fisico-Matematicas, Universidad Autónoma de Sinaloa, Culiacan (Mexico); Midttun, Øystein [CERN, Geneva (Switzerland); University of Oslo, Oslo (Norway)

    2014-02-15

    The space charge effect of low energy, unbunched ion beams can be compensated by the trapping of ions or electrons into the beam potential. This has been studied for the 45 keV negative hydrogen ion beam in the CERN Linac4 Low Energy Beam Transport using the package IBSimu [T. Kalvas et al., Rev. Sci. Instrum. 81, 02B703 (2010)], which allows the space charge calculation of the particle trajectories. The results of the beam simulations will be compared to emittance measurements of an H{sup −} beam at the CERN Linac4 3 MeV test stand, where the injection of hydrogen gas directly into the beam transport region has been used to modify the space charge compensation degree.

  18. AP physics B crash course

    CERN Document Server

    Howell, Rebecca

    2012-01-01

    AP Physics B Crash Course - Get a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. Our AP Physics B Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know The Crash Course is based on an in-depth analysis of the AP Physics B course description outline and actual AP test questions. It covers only the information tested on the exam, so you can make the most of your valuable study time. Our easy-to-read format covers: mechanics, kinetic theory, t

  19. Analysis of sediment transport pattern along the coastal line of the Curonian Spit

    Science.gov (United States)

    Kovaleva, Olga; Chubarenko, Boris

    2016-04-01

    Among a wide range of approaches for determination of long-shore sediment transport direction changes in grain-size parameters (mean, sorting, skewness) of beach deposits use not so often. There are two trends in alongshore distributions of surface sediments grain-size parameters which may indicate an alongshore transport (McLaren, Bowles, 1985): (a) sediments become finer, sorting and skewness decreases; (b) sediments become coarser, sorting decreases and skewness increases. Over all more than 150 sand samples were collected along the coastline of the Curonian Spit during 2011, 2014 and 2015 summer seasons. The grain-size analysis of the samples were carried out and mentioned above grain-size parameters were calculated. Shore segments with different trends were identified using a running 9-nodes window, level of significance of trend analysis was estimated. Results of the method testing reflected different sediment transportation patterns for different years. However for other areas such as embayed coasts at the Gulf of Finland this method showed presence of one-directed longshore sediment flow. As a result it is possible to say that for straightened coasts such as the Curonian Spit the method allows to determine the alongshore flux pattern formed by the last significant storm event, i.e. to estimate an actual consequence of winds and waves influence in the studied area and can not be used for describing multiannual longshore sediment transport. The work was financed by the Russian Scientific Fund (grant 14-37-00047) and by the Russian Foundation for Basic Research, research projects No. 14-35-50130 and No. 15-35-50613.

  20. Center for Geometrisk Metrologi, CGM ApS

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2002. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, Erhvervsfremme Styrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler (Teknisk Forskrift Nr. TF4 af 2000...

  1. Army and Marine Corps Active Protection System (APS) Efforts

    Science.gov (United States)

    2016-08-30

    Expedited NDI effort. The Marines have a number of unique APS requirements—including the ability to be transported by ship and withstand salt water...in urban environments. Such constraints could limit the usefulness of APS in certain combat scenarios. Collateral Damage In a manner similar to...fired at short range, in open area or urban environment, AT Guided Missiles, High Explosive AT and Kinetic Energy rounds. The Iron Fist is

  2. High intensity proton beam transportation through fringe field of 70 MeV compact cyclotron to beam line targets

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xu, E-mail: emmazhang103@gmail.com [China Institute of Atomic Energy (China); Li, Ming; Wei, Sumin; Xing, Jiansheng; Hu, Yueming [China Institute of Atomic Energy (China); Johnson, Richard R.; Piazza, Leandro; Ryjkov, Vladimir [BEST Cyclotron Inc (Canada)

    2016-06-01

    From the stripping points, the high intensity proton beam of a compact cyclotron travels through the fringe field area of the machine to the combination magnet. Starting from there the beams with various energy is transferred to the switching magnet for distribution to the beam line targets. In the design of the extraction and transport system for the compact proton cyclotron facilities, such as the 70 MeV in France and the 100 MeV in China, the space charge effect as the beam crosses the fringe field has not been previously considered; neither has the impact on transverse beam envelope coupled from the longitudinal direction. Those have been concerned much more with the higher beam-power because of the beam loss problem. In this paper, based on the mapping data of 70 MeV cyclotron including the fringe field by BEST Cyclotron Inc (BEST) and combination magnet field by China Institute of Atomic Energy (CIAE), the beam extraction and transport are investigated for the 70 MeV cyclotron used on the SPES project at Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Legnaro (INFN–LNL). The study includes the space charge effect and longitudinal and transverse coupling mentioned above, as well as the matching of beam optics using the beam line for medical isotope production as an example. In addition, the designs of the ±45° switching magnets and the 60° bending magnet for the extracted beam with the energy from 35 MeV to 70 MeV have been made. Parts of the construction and field measurements of those magnets have been done as well. The current result shows that, the design considers the complexity of the compact cyclotron extraction area and fits the requirements of the extraction and transport for high intensity proton beam, especially at mA intensity levels.

  3. High intensity proton beam transportation through fringe field of 70 MeV compact cyclotron to beam line targets

    Science.gov (United States)

    Zhang, Xu; Li, Ming; Wei, Sumin; Xing, Jiansheng; Hu, Yueming; Johnson, Richard R.; Piazza, Leandro; Ryjkov, Vladimir

    2016-06-01

    From the stripping points, the high intensity proton beam of a compact cyclotron travels through the fringe field area of the machine to the combination magnet. Starting from there the beams with various energy is transferred to the switching magnet for distribution to the beam line targets. In the design of the extraction and transport system for the compact proton cyclotron facilities, such as the 70 MeV in France and the 100 MeV in China, the space charge effect as the beam crosses the fringe field has not been previously considered; neither has the impact on transverse beam envelope coupled from the longitudinal direction. Those have been concerned much more with the higher beam-power because of the beam loss problem. In this paper, based on the mapping data of 70 MeV cyclotron including the fringe field by BEST Cyclotron Inc (BEST) and combination magnet field by China Institute of Atomic Energy (CIAE), the beam extraction and transport are investigated for the 70 MeV cyclotron used on the SPES project at Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Legnaro (INFN-LNL). The study includes the space charge effect and longitudinal and transverse coupling mentioned above, as well as the matching of beam optics using the beam line for medical isotope production as an example. In addition, the designs of the ±45° switching magnets and the 60° bending magnet for the extracted beam with the energy from 35 MeV to 70 MeV have been made. Parts of the construction and field measurements of those magnets have been done as well. The current result shows that, the design considers the complexity of the compact cyclotron extraction area and fits the requirements of the extraction and transport for high intensity proton beam, especially at mA intensity levels.

  4. Space Charge Compensation in the Linac4 Low Energy Beam Transport Line with Negative Hydrogen Ions

    CERN Document Server

    Valerio-Lizarraga, C; Leon-Monzon, I; Lettry, J; Midttun, O; Scrivens, R

    2014-01-01

    The space charge effect of low energy, unbunched ion beams can be compensated by the trapping of ions or electrons into the beam potential. This has been studied for the 45 keV negative hydrogen ion beam in the CERN Linac4 Low Energy Beam Tranport (LEBT) using the package IBSimu1, which allows the space charge calculation of the particle trajectories. The results of the beam simulations will be compared to emittance measurements of an H- beam at the CERN Linac4 3 MeV test stand, where the injection of hydrogen gas directly into the beam transport region has been used to modify the space charge compensation degree.

  5. Learning about Antiphospholipid Syndrome (APS)

    Science.gov (United States)

    ... other autoimmune disorders, including myasthenia gravis, Graves' disease, autoimmune hemolytic anemia, and Evan's syndrome. Top of page How is APS diagnosed? A diagnosis of APS is made based on both clinical and ... Autoimmune Related Diseases Association, Inc. [aarda.org] An organization ...

  6. Differential effects and transport kinetics of ascorbate derivatives in leukemic cell lines.

    Science.gov (United States)

    Koh, W S; Lee, S J; Lee, H; Park, C; Park, M H; Kim, W S; Yoon, S S; Park, K; Hong, S I; Chung, M H; Park, C H

    1998-01-01

    In order to investigate the differential effects of ascorbate derivatives on leukemic cell growth, we examined their stabilities and transmembrane transport efficiencies. The growth of HL-60 and U937 cells was dose-dependently inhibited by ascorbic acid and sodium ascorbate, but not by dehydroascorbic acid and magnesium ascorbyl 2-phosphate up to 200 microM. The growth-suppression by ascorbic acid was dependent on its redox state, showing a complete or partial reversion by ascorbate oxidase or FeCl3 addition, respectively. Three different patterns of intracellular ascorbic acid accumulation were observed by HPLC according to the species of ascorbate derivative applied for the incubation. Compared with the reduced form of ascorbic acid, the oxidized forms (dehydroascorbic acid, ascorbic acid plus ascorbate oxidase or FeCl3) were rapidly transported into cells and readily degraded, while magnesium ascorbyl 2-phosphate, a stable derivative of ascorbic acid, slowly elevated the intracellular level of ascorbic acid, reaching a plateau at 24 hours. We also measured the differential kinetics of ascorbic acid levels In culture supernatants following the addition of ascorbate derivatives. Ascorbic acid at 40, 10, or 1 microM was observed 3 hours following treatment with 100 microM of ascorbic acid, ascorbic acid plus FeCl3, or magnesium ascorbyl 2-phosphate, respectively. No ascorbic acid was found in the culture supernatant treated with dehydroascorbic acid. This order of ascorbic acid concentrations in culture supernatant reflects their growth-inhibitory effects. Thus the growth inhibitory effect of ascorbic acid appears to be dependent on its concentration in culture medium rather than its intracellular concentration. In conclusion, the results in this study indicate that the differential effects of ascorbate derivatives appear to be due to the actual concentration differences of the reduced form of ascorbic acid in culture medium following their addition, which is

  7. The GEANT4 toolkit capability in the hadron therapy field: simulation of a transport beam line

    Science.gov (United States)

    Cirrone, G. A. P.; Cuttone, G.; Di Rosa, F.; Raffaele, L.; Russo, G.; Guatelli, S.; Pia, M. G.

    2006-01-01

    At Laboratori Nazionali del Sud of the Instituto Nazionale di Fisica Nucleare of Catania (Sicily, Italy), the first Italian hadron therapy facility named CATANA (Centro di AdroTerapia ed Applicazioni Nucleari Avanzate) has been realized. Inside CATANA 62 MeV proton beams, accelerated by a superconducting cyclotron, are used for the radiotherapeutic treatments of some types of ocular tumours. Therapy with hadron beams still represents a pioneer technique, and only a few centers worldwide can provide this advanced specialized cancer treatment. On the basis of the experience so far gained, and considering the future hadron-therapy facilities to be developed (Rinecker, Munich Germany, Heidelberg/GSI, Darmstadt, Germany, PSI Villigen, Switzerland, CNAO, Pavia, Italy, Centro di Adroterapia, Catania, Italy) we decided to develop a Monte Carlo application based on the GEANT4 toolkit, for the design, the realization and the optimization of a proton-therapy beam line. Another feature of our project is to provide a general tool able to study the interactions of hadrons with the human tissue and to test the analytical-based treatment planning systems actually used in the routine practice. All the typical elements of a hadron-therapy line, such as diffusers, range shifters, collimators and detectors were modelled. In particular, we simulated the Markus type ionization chamber and a Gaf Chromic film as dosimeters to reconstruct the depth (Bragg peak and Spread Out Bragg Peak) and lateral dose distributions, respectively. We validated our simulated detectors comparing the results with the experimental data available in our facility.

  8. Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.

    Directory of Open Access Journals (Sweden)

    Tiago dos Santos

    Full Text Available Nanotechnology is expected to play a vital role in the rapidly developing field of nanomedicine, creating innovative solutions and therapies for currently untreatable diseases, and providing new tools for various biomedical applications, such as drug delivery and gene therapy. In order to optimize the efficacy of nanoparticle (NP delivery to cells, it is necessary to understand the mechanisms by which NPs are internalized by cells, as this will likely determine their ultimate sub-cellular fate and localisation. Here we have used pharmacological inhibitors of some of the major endocytic pathways to investigate nanoparticle uptake mechanisms in a range of representative human cell lines, including HeLa (cervical cancer, A549 (lung carcinoma and 1321N1 (brain astrocytoma. Chlorpromazine and genistein were used to inhibit clathrin and caveolin mediated endocytosis, respectively. Cytochalasin A and nocodazole were used to inhibit, respectively, the polymerisation of actin and microtubule cytoskeleton. Uptake experiments were performed systematically across the different cell lines, using carboxylated polystyrene NPs of 40 nm and 200 nm diameters, as model NPs of sizes comparable to typical endocytic cargoes. The results clearly indicated that, in all cases and cell types, NPs entered cells via active energy dependent processes. NP uptake in HeLa and 1321N1 cells was strongly affected by actin depolymerisation, while A549 cells showed a stronger inhibition of NP uptake (in comparison to the other cell types after microtubule disruption and treatment with genistein. A strong reduction of NP uptake was observed after chlorpromazine treatment only in the case of 1321N1 cells. These outcomes suggested that the same NP might exploit different uptake mechanisms to enter different cell types.

  9. A transverse emittance and acceptance measurement system in a low-energy beam transport line

    Energy Technology Data Exchange (ETDEWEB)

    Kashiwagi, H., E-mail: kashiwagi.hirotsugu@jaea.go.jp; Miyawaki, N.; Kurashima, S.; Okumura, S. [Department of Advanced Radiation Technology, Japan Atomic Energy Agency, 1233 Watanuki, Takasaki, Gunma 370-1292 (Japan)

    2014-02-15

    A transverse beam emittance and acceptance measurement system has been developed to visualize the relationship between the injected beam emittance and the acceptance of a cyclotron. The system is composed of a steering magnet, two pairs of slits to limit the horizontal and vertical phase-space, a beam intensity detector just behind the slits for the emittance measurement, and a beam intensity detector in the cyclotron for the acceptance measurement. The emittance is obtained by scanning the slits and measuring the beam intensity distribution. The acceptance is obtained by measuring the distribution of relative beam transmission by injecting small emittance beams at various positions in a transverse phase-space using the slits. In the acceptance measurement, the beam from an ion source is deflected to the defined region by the slits using the steering magnet so that measurable acceptance area covers a region outside the injection beam emittance. Measurement tests were carried out under the condition of accelerating a beam of {sup 16}O{sup 6+} from 50.2 keV to 160 MeV. The emittance of the injected beam and the acceptance for accelerating and transporting the beam to the entrance of the extraction deflector were successfully measured. The relationship between the emittance and acceptance is visualized by displaying the results in the same phase-plane.

  10. APS Editorial Q&A: APS and Open Access

    Science.gov (United States)

    2011-04-01

    The movement toward Open Access continues to gain momentum. A brief review of APS efforts in this area will be presented by APS Editor in Chief, Gene Sprouse. Editors from Physical Review C, D, Letters, and X, Physical Review Special Topics - Accelerators and Beams, Physical Review Special Topics - Physics Education Research, and Reviews of Modern Physics will address your questions about publishing in this evolving environment.

  11. SN 2005ap: A Most Brilliant Explosion

    CERN Document Server

    Quimby, Robert M; Wheeler, J Craig; Höflich, Peter; Akerlof, Carl W; Rykoff, Eli S

    2007-01-01

    We present unfiltered photometric observations with ROTSE-III and optical spectroscopic follow-up with the HET and Keck of the most luminous supernova yet identified, SN 2005ap. The spectra taken about 3 days before and 6 days after maximum light show narrow emission lines (likely originating in the dwarf host) and absorption lines at a redshift of z=0.2832, which puts the peak unfiltered magnitude at -22.7 +/- 0.1 absolute. Broad P-Cygni features corresponding to H-alpha, CIII, NIII, and OIII, are further detected with a photospheric velocity of ~20,000 km/s. Unlike other highly luminous supernovae such as 2006gy and 2006tf that show slow photometric evolution, the light curve of SN 2005ap indicates a 1-3 week rise to peak followed by a relatively rapid decay. The spectra also lack the distinct emission peaks from moderately broadened (FWHM ~ 2,000 km/s) Balmer lines seen in SN 2006gy and SN 2006tf. We briefly discuss the origin of the extraordinary luminosity from a strong interaction as may be expected fro...

  12. Beam dynamics and error study of the medium energy beam transport line in the Korea Heavy-Ion Medical Accelerator

    Science.gov (United States)

    Kim, Chanmi; Kim, Eun-San; Hahn, Garam

    2016-11-01

    The Korea Heavy Ion Medical Accelerator consists of an injector and a synchrotron for an ion medical accelerator that is the first carbon-ion therapy system in Korea. The medium energy beam transport(MEBT) line connects the interdigital H-mode drift tube linac and the synchrotron. We investigated the beam conditions after the charge stripper by using the LISE++ and the SRIM codes. The beam was stripped from C4+ into C6+ by using the charge stripper. We investigated the performance of a de-buncher in optimizing the energy spread and the beam distribution in z-dW/W (direction of beam progress-beam and energy) phase. We obtained the results of the tracking simulation and the error analysis by using the TRACK code. Possible misalignments and rotations of the magnets were considered in the simulations. States of the beam were examined when errors occurred in the magnets by the applying analytic fringe field model in TRACK code. The condition for the beam orbit was optimized by using correctors and profile monitors to correct the orbit. In this paper, we focus on the beam dynamics and the error studies dedicated to the MEBT beam line and show the optimized beam parameters for the MEBT.

  13. Design of the prototype of a beam transport line for handling and selection of low energy laser-driven beams

    Science.gov (United States)

    Schillaci, F.; Maggiore, M.; Cirrone, G. A. P.; Cuttone, G.; Pisciotta, P.; Costa, M.; Rifuggiato, D.; Romano, F.; Scuderi, V.

    2016-11-01

    A first prototype of transport beam-line for laser-driven ion beams to be used for the handling of particles accelerated by high-power laser interacting with solid targets has been realized at INFN. The goal is the production of a controlled and stable beam in terms of energy and angular spread. The beam-line consists of two elements: an Energy Selection System (ESS), already realized and characterized with both conventional and laser-accelerated beams, and a Permanent Magnet Quadrupole system (PMQ) designed, in collaboration with SIGMAPHI (Fr), to improve the ESS performances. In this work a description of the ESS system and some results of its characterization with conventional beams are reported, in order to provide a complete explanation of the acceptance calculation. Then, the matching with the PMQ system is presented and, finally, the results of preliminary simulations with a realistic laser-driven energy spectrum are discussed demonstrating the possibility to provide a good quality beam downstream the systems.

  14. Plant-specific insertions in the soybean aspartic proteinases, soyAP1 and soyAP2, perform different functions of vacuolar targeting.

    Science.gov (United States)

    Terauchi, Kaede; Asakura, Tomiko; Ueda, Haruko; Tamura, Tomoko; Tamura, Kentaro; Matsumoto, Ichiro; Misaka, Takumi; Hara-Nishimura, Ikuko; Abe, Keiko

    2006-07-01

    Most aspartic proteinases (APs) of plant origin are characterized by the presence of plant-specific insertion (PSI) in their primary structure. PSI has been reported to function as signals for both transport of AP molecules from the endoplasmic reticulum (ER) and for their targeting to the vacuole. To determine the functions of the PSIs in soyAP1 and soyAP2 identified in our previous study, we examined their subcellular localization by transient expression of a green fluorescent protein (GFP) fusion protein in the protoplasts of Arabidopsis suspension-cultured cells. Both soyAP1-GFP and soyAP2-GFP were targeted to the vacuole. To confirm the role of the PSI, we prepared PSI-deleted soyAP1 and soyAP2, and investigated their vacuolar targeting by the same method. While the former deletion mutant was always transported to the vacuole, the latter sometimes remained in the ER and was only sometimes transported to the vacuole. These observations indicated that, in the case of soyAP1, the PSI is not involved in vacuolar targeting, also suggesting that the function of the PSI differs depending on its origin.

  15. Railroad Lines - RAIL_SYSTEM_ACTIVE_ABANDONED_INDOT_IN: Active and Abandoned Rail System in Indiana, 2005 (Indiana Department of Transportation, 1:1200, Line Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — RAIL_SYSTEM_ACTIVE_ABANDONED_INDOT_IN is a line shapefile that contains all active and abandoned rail lines in Indiana, provided by personnel of Indiana Department...

  16. Long-term mean and seasonal variation of altimetry-derived Oyashio transport across the A-line off the southeastern coast of Hokkaido, Japan

    Science.gov (United States)

    Kuroda, Hiroshi; Wagawa, Taku; Kakehi, Shigeho; Shimizu, Yugo; Kusaka, Akira; Okunishi, Takeshi; Hasegawa, Daisuke; Ito, Shin-ichi

    2017-03-01

    To understand long-term mean and seasonal variations of the Oyashio transport off the southeastern coast of Hokkaido, time series of estimated transports were generated by combining satellite altimetry and CTD data from the period 1993-2014 along a monitoring transect referred to as the ;A-line;. Linear regressions between CTD-derived geostrophic transport and altimetry-derived sea level anomaly differences were calculated by minimizing a cost function newly proposed in this study. The horizontal structure of 21-year mean transport was characterized by a southwestward flow on the continental slope (SW-1) associated with the First Oyashio Intrusion, a northeastward flow (NE-2) likely trapped against the Kuril-Kamchatka trench, a southwestward flow (SW-3) related to the Second Oyashio Intrusion, and a very stable northeastward flow (NE-4) corresponding to a combination of the Oyashio Return Flow and quasi-stationary Jet. Analysis of long-term mean monthly transports revealed four alternating flows that were present throughout all seasons of the year. The boundaries between adjacent pairs of the four flows on the A-line did not change on a seasonal timescale as much as the southernmost latitude of the First Oyashio Intrusion. At a 0-month (1-month) time lag, the sum of the SW-1, NE-2, and SW-3 (SW-1 and NE-2) transports, which corresponds to net Oyashio transport (partial net Oyashio transport related to the southernmost latitude of the First Oyashio Intrusion), was correlated with Sverdrup transport more robustly than was each of the four alternating flows. This result suggests that as the Oyashio crossed the A-line, it responded barotropically to basin-scale wind stress by adjusting transports composed of both southwestward and northeastward flows.

  17. Epidermal growth factor decreases PEPT2 transport capacity and expression in the rat kidney proximal tubule cell line SKPT0193 cl.2

    DEFF Research Database (Denmark)

    Bravo, Silvina A; Nielsen, Carsten Uhd; Amstrup, Jan;

    2004-01-01

    transport capacity and expression in the rat proximal tubule cell line SKPT0193 cl.2 (SKPT), which expresses rat PEPT2 (rPEPT2) in the apical membrane. Treatment of SKPT cells with EGF during cell culture growth caused a dose-dependent decrease in rPEPT2 transport capacity and expression, as determined...... suggests that this might be disadvantageous when studying PEPT2-mediated transport phenomena. These findings demonstrate for the first time EGF-mediated regulation of PEPT2 expression in a kidney cell line. The relevance for kidney regulation of peptide transport activity in physiological and...... by studies of apical uptake of [14C]glycylsarcosine, rPepT2 mRNA levels, and immunostaining of SKPT cells with a rPEPT2-specific antibody. On the contrary, apical uptake of glucose and lysine was increased in EGF-treated cells, indicating that EGF was not acting generally to decrease apical nutrient uptake...

  18. Identification of Cargo for Adaptor Protein (AP) Complexes 3 and 4 by Sucrose Gradient Profiling.

    Science.gov (United States)

    Pertl-Obermeyer, Heidi; Wu, Xu Na; Schrodt, Jens; Müdsam, Christina; Obermeyer, Gerhard; Schulze, Waltraud X

    2016-09-01

    Intracellular vesicle trafficking is a fundamental process in eukaryotic cells. It enables cellular polarity and exchange of proteins between subcellular compartments such as the plasma membrane or the vacuole. Adaptor protein complexes participate in the vesicle formation by specific selection of the transported cargo. We investigated the role of the adaptor protein complex 3 (AP-3) and adaptor protein complex 4 (AP-4) in this selection process by screening for AP-3 and AP-4 dependent cargo proteins. Specific cargo proteins are expected to be mis-targeted in knock-out mutants of adaptor protein complex components. Thus, we screened for altered distribution profiles across a density gradient of membrane proteins in wild type versus ap-3β and ap-4β knock-out mutants. In ap-3β mutants, especially proteins with transport functions, such as aquaporins and plasma membrane ATPase, as well as vesicle trafficking proteins showed differential protein distribution profiles across the density gradient. In the ap-4β mutant aquaporins but also proteins from lipid metabolism were differentially distributed. These proteins also showed differential phosphorylation patterns in ap-3β and ap-4β compared with wild type. Other proteins, such as receptor kinases were depleted from the AP-3 mutant membrane system, possibly because of degradation after mis-targeting. In AP-4 mutants, membrane fractions were depleted for cytochrome P450 proteins, cell wall proteins and receptor kinases. Analysis of water transport capacity in wild type and mutant mesophyll cells confirmed aquaporins as cargo proteins of AP-3 and AP-4. The combination of organelle density gradients with proteome analysis turned out as a suitable experimental strategy for large-scale analyses of protein trafficking.

  19. Task Force on Catastrophic Antiphospholipid Syndrome (APS) and Non-criteria APS Manifestations (I): catastrophic APS, APS nephropathy and heart valve lesions.

    Science.gov (United States)

    Cervera, R; Tektonidou, M G; Espinosa, G; Cabral, A R; González, E B; Erkan, D; Vadya, S; Adrogué, H E; Solomon, M; Zandman-Goddard, G; Shoenfeld, Y

    2011-02-01

    The objectives of the 'Task Force on Catastrophic Antiphospholipid Syndrome (APS) and Non-criteria APS Manifestations' were to assess the clinical utility of the international consensus statement on classification criteria and treatment guidelines for the catastrophic APS, to identify and grade the studies that analyse the relationship between the antiphospholipid antibodies and the non-criteria APS manifestations and to present the current evidence regarding the accuracy of these non-criteria APS manifestations for the detection of patients with APS. This article summarizes the studies analysed on the catastrophic APS, APS nephropathy and heart valve lesions, and presents the recommendations elaborated by the Task Force after this analysis.

  20. Stratification in Ap star atmospheres: Simulations

    Science.gov (United States)

    Cowley, Charles R.; Castelli, Fiorella

    2017-01-01

    It is now well established that the atmospheres of Ap stars can be chemically stratified (cf. Babel, A\\&A 258, 645, 1992; Ryabchikova et al. A\\&A 384, 545, 2002). The most convincing cases have been made with the profiles of very strong lines, such as Ca II K. Weaker line profiles are less obvious indicators. The collective behavior of sets or groups of lines have also been used. For example, if higher abundances are derived for strong lines in an atmosphere with zero microturbulence, one may assume that the absorbing species has been pushed into the higher photospheres. An example are the medium-strong Mn II lines in HgMn stars. In this paper, we probe this assumption by calculating line strengths with various assumed stratification models, and then determining abundances from those lines using an {\\bf unstratified} model with the same Teff and log(g). We use the model from Castelli, Kurucz \\& and Hubrig (A\\&A, 508, 401, 2009) for HR 6000, whose spectrum shows numerous indications of stratification. A variety of stratification models are considered, for example, ones where the majority of an absorbing species is concentrated above (or below) $log(\\tau_{5000}$ = -2.0. Cloud models are also investigated, where a species is concentrated within a range of photospheric depths. Curves of growth are generated in unstratified atmospheres for lines by holding the abundance fixed, and increasing log(gf). Similar curves are made in stratified models, and the ratios of strong to weak linesare compared with and without stratification. The effects of stratification on ionization are also investigated, as well as on the profiles of strong lines. We find, in agreement with previous work, that severe abundance jumps are sometimes required to account for some of the observed peculiarities.

  1. 一种新的小檗碱衍生物CPU-86017在人小肠上皮细胞(Caco-2细胞)中的转运与摄取特性%Transport and uptake characteristics of a new derivative of berberine (CPU-86017) by human intestinal epithelial cell line: Caco-2

    Institute of Scientific and Technical Information of China (English)

    杨海涛; 王广基

    2003-01-01

    AIM: The characteristics of transepithelial transport and uptake of CPU-86017 {[7-(4-chlorbenzyl)-7,8,13,13α tetrahydroberberine chloride, CTHB] }, a new antiarrhythmia agent and a new derivative of berberine, were investi gated on epithelial cell line (Caco-2) to further understand the absorption mechanism of berberine and its derivatives. METHODS: Caco-2 cell was used. RESULTS: 1) The permeability coefficient from the apical (AP) to basolateral (BL) of CPU-86017 was approximately 5 times higher than that from BL-to-AP transport. The effects of a P-glycoprotein (P-gp) inhibitor-cyclosporin A, some surfactants, and lower pH on the transepithelial transport of CPU-86017 were also observed. Cyclosporine A at 7.5 mg/L had no effect on the transepithelial electrical resistance (TEER); an about 4-fold enhancement on the transepithlial transport of CPU-86017 was observed. Some surfac tants (sodium citrate, sodium deoxycholate, and sodium dodecyl sulfate) at 100 μmol/L and low pH (pH=6.0) induced a reversible decrease of TEER; enhancements of the transepithelial transport of CPU-86017 were also observed with some surfactants; 2) In the process of uptake of CPU-86017, the initial uptake rates of CPU-86017 were saturable with a Vmax of (250+39) μg.min-1.g-1 (protein) and Km of (0.90+0.12) mmol/L. This process was enhanced by cyclosporine A (7.5 mg/L) with a Vmax of (588+49) μg.min-1.g-1 (protein) and Km (0.42+0.08) mmol/L. CONCLUSION: Some surfactants and P-gp inhibitors can be considered as enhancers of its transepithelial trans port and uptake.

  2. Epidermal growth factor inhibits glycyl sarcosine transport and hPepT1 expression in a human intestinal cell line

    DEFF Research Database (Denmark)

    Nielsen, Carsten Uhd; Amstrup, Jan; Steffansen, Bente;

    2001-01-01

    Intestinal oligopeptide transporter, growth factor, immunocytochemistry, laser scanning confocal microscopy......Intestinal oligopeptide transporter, growth factor, immunocytochemistry, laser scanning confocal microscopy...

  3. Insulin sensitivity and inhibition by forskolin, dipyridamole and pentobarbital of glucose transport in three L6 muscle cell lines

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    L6 skeletal muscle myoblasts stably overexpressing glucose transporter GLUT1 or GLUT4 with exofacial myc-epitope tags were characterized for their response to insulin. In clonally selected cultures, 2-deoxyglucose uptake into L6-GLUT1myc myoblasts and myotubes was linear within the time of study. In L6-GLUT1myc and L6-GLUT4myc myoblasts, 100 nmol/L insulin treatment increased the GLUT1 content of the plasma membrane by 1.58±0.01 fold and the GLUT4 content 1.96±0.11 fold, as well as the 2-deoxyglucose uptake 1.53±0.09 and 1.86±0.17 fold respectively, all by a wortmannin-inhibitable manner. The phosphorylation of Akt in these two cell lines was increased by insulin. L6-GLUT1myc myoblasts showed a dose-dependent stimulation of glucose uptake by insulin, with unaltered sensitivity and maximal responsiveness compared with wild type cells. By contrast, the improved insulin responsiveness and sensitivity of glucose uptake were observed in L6-GLUT4myc myoblasts. Earlier studies indicated that forskolin might affect insulin-stimulated GLUT4 translocation. A 65% decrease of insulin-stimulated 2-deoxyglucose uptake in GLUT4myc cells was not due to an effect on GLUT4 mobilization to the plasma membrane, but instead on direct inhibition of GLUT4. Forskolin and dipyridamole are more potent inhibitors of GLUT4 than GLUT1. Alternatively, pentobarbital inhibits GLUT1 more than GLUT4. The use of these inhibitors confirmed that the overexpressed GLUT1 or GLUT4 are the major functional glucose transporters in unstimulated and insulin-stimulated L6 myoblasts. Therefore, L6-GLUT1myc and L6-GLUT4myc cells provide a platform to screen compounds that may have differential effects on GLUT isoform activity or may influence GLUT isoform mobilization to the cell surface of muscle cells.

  4. Transcellular transport of 4-iodo-L-meta-tyrosine via system L across monolayers of kidney epithelial cell line LLC-PK{sub 1}

    Energy Technology Data Exchange (ETDEWEB)

    Shikano, Naoto E-mail: sikano@ipu.ac.jp; Kawai, Keiichi; Nakajima, Syuichi; Kubodera, Akiko; Kubota, Nobuo; Ishikawa, Nobuyoshi; Saji, Hideo

    2004-05-01

    The substance 4-[{sup 125}I]iodo-L-meta-tyrosin (4-[{sup 125}I]mTyr) is a radioiodinated amino acid that exhibits high in vivo stability and rapid renal elimination in vivo. We investigated transport of 4-[{sup 125}I]mTyr in LLC-PK{sub 1} (porcine kidney epithelial cell line) monolayers grown on collagen-coated, micro-porous membrane filters. We found that 4-[{sup 125}I]mTyr transport in LLC-PK{sub 1} cells was carrier-mediated and sodium-independent, and that 4-[{sup 125}I]mTyr transport was similar to that of L-Tyr and 3-iodo-{alpha}-methyl-L-tyrosine. The results of the inhibition experiments suggest that 4-[{sup 125}I]mTyr transport is predominantly mediated by a L-type amino acid transporter 1-like porcine homologue (a component of system L) in both basolateral and apical membrane.

  5. [Apheresis in antiphospholipid syndrome (APS)].

    Science.gov (United States)

    De Silvestro, Giustina; Tison, Tiziana; Marson, Piero

    2012-01-01

    Antiphospholipid syndrome (APS) is a rare clinical disorder characterized by thromboembolic manifestations and/or obstetric complications. Along with the clinical symptoms and signs, serum antiphospholipid antibodies have to be detected. APS can be primary, i.e., without any concomitant disorders, or secondary to other autoimmune diseases, particularly systemic lupus erythematosus. Criteria for the diagnosis of APS have been clearly established. Hyperacute APS (or catastrophic antiphospholipid syndrome), often with a poor prognosis, must meet four criteria: involvement of three or more organs, rapid evolution of clinical manifestations, microangiopathic occlusion of small blood vessels at biopsy, and presence of antiphospholipid antibodies. The rationale for apheresis treatment is the removal of pathogenetic antibodies involved in the development of tissue damage. Our experience includes 23 patients, in particular 15 women treated for 19 pregnancies. According to the National Guidelines Program, the effectiveness of apheresis in catastrophic syndrome has a level of evidence of V/VI, with a strength of recommendation A; in highrisk pregnancy it has a level of evidence of V with a strength of recommendation B. It will be necessary to better define the prognosis of various categories of pregnant patients with APS, as well as useful laboratory parameters to monitor its clinical course and anticipate any complications of pregnancy.

  6. APS Education and Diversity Efforts

    Science.gov (United States)

    Prestridge, Katherine; Hodapp, Theodore

    2015-11-01

    American Physical Society (APS) has a wide range of education and diversity programs and activities, including programs that improve physics education, increase diversity, provide outreach to the public, and impact public policy. We present the latest programs spearheaded by the Committee on the Status of Women in Physics (CSWP), with highlights from other diversity and education efforts. The CSWP is working to increase the fraction of women in physics, understand and implement solutions for gender-specific issues, enhance professional development opportunities for women in physics, and remedy issues that impact gender inequality in physics. The Conferences for Undergraduate Women in Physics, Professional Skills Development Workshops, and our new Professional Skills program for students and postdocs are all working towards meeting these goals. The CSWP also has site visit and conversation visit programs, where department chairs request that the APS assess the climate for women in their departments or facilitate climate discussions. APS also has two significant programs to increase participation by underrepresented minorities (URM). The newest program, the APS National Mentoring Community, is working to provide mentoring to URM undergraduates, and the APS Bridge Program is an established effort that is dramatically increasing the number of URM PhDs in physics.

  7. Investigation of transport of genistein, daidzein and their inclusion complexes prepared with different cyclodextrins on Caco-2 cell line.

    Science.gov (United States)

    Daruházi, Agnes Emma; Kiss, Tímea; Vecsernyés, Miklós; Szente, Lajos; Szőke, Eva; Lemberkovics, Eva

    2013-10-01

    Isoflavonoids are widespread constituents in medical plants especially in legumes (Fabaceae), but occur in other different plant families as well (Rosaceae, Iridaceae, Amaranthaceae). Their antioxidant, estrogen-like, anti-inflammatory and analgesic effects make them promising compounds in therapy of important disorders especially in estrogen related diseases. Poor solubility in aqueous system of genistein and daidzein needs a solubility enhancement for pharmaceutical use. These compounds are suitable guest molecules for inclusion complex formation with cyclodextrins (CDs) considering matching their size and polarity. The molecular encapsulation with beta-cyclodextrin (β-CD), gamma-cyclodextrin (γ-CD), hydroxypropyl-beta-cyclodextrin (HP-β-CD) and random methyl-beta cyclodextrin (RAMEB-CD) results in a solid, molecularly dispersed form and in a significantly improved aqueous solubility of genistein and daidzein. Determining enhancement in solubility and bioavailability we investigated the transport of these inclusion complexes across Caco-2 cell line comparing that of the pure compounds and found significant improving effect of the different CD derivatives on membrane permeation of the two isoflavone aglycons.

  8. Monolayers of IEC-18 cells as an in vitro model for screening the passive transcellular and paracellular transport across the intestinal barrier: Comparison of active and passive transport with the human colon carcinoma Caco-2 cell line

    NARCIS (Netherlands)

    Versantvoort, C.H.M.; Ondrewater, R.C.A.; Duizer, E.; Sandt, J.J.M. van de; Gilde, A.J.; Groten, J.P.

    2002-01-01

    Purpose: previous studies have shown that the rat small intestinal cell line IEC-18 provides a size-selective barrier for paracellularly transported hydrophilic macromolecules. In order to determine the utility of IEC-18 cells as an in vitro model to screen the passive paracellular and transcellular

  9. Design of a high-current low-energy beam transport line for an intense D-T/D-D neutron generator

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Xiaolong, E-mail: luxl@lzu.edu.cn [School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000 (China); Engineering Research Center for Neutron Application, Ministry of Education, Lanzhou University, Lanzhou 730000 (China); Wang, Junrun [School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000 (China); Engineering Research Center for Neutron Application, Ministry of Education, Lanzhou University, Lanzhou 730000 (China); Zhang, Yu; Li, Jianyi; Xia, Li; Zhang, Jie; Ding, Yanyan; Jiang, Bing; Huang, Zhiwu; Ma, Zhanwen; Wei, Zheng [School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000 (China); Qian, Xiangping; Xu, Dapeng; Lan, Changlin [School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000 (China); Engineering Research Center for Neutron Application, Ministry of Education, Lanzhou University, Lanzhou 730000 (China); Yao, Zeen, E-mail: zeyao@lzu.edu.cn [School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000 (China); Engineering Research Center for Neutron Application, Ministry of Education, Lanzhou University, Lanzhou 730000 (China)

    2016-03-01

    An intense D-T/D-D neutron generator is currently being developed at the Lanzhou University. The Cockcroft–Walton accelerator, as a part of the neutron generator, will be used to accelerate and transport the high-current low-energy beam from the duoplasmatron ion source to the rotating target. The design of a high-current low-energy beam transport (LEBT) line and the dynamics simulations of the mixed beam were carried out using the TRACK code. The results illustrate that the designed beam line facilitates smooth transportation of a deuteron beam of 40 mA, and the number of undesired ions can be reduced effectively using two apertures.

  10. Design of medium energy beam transport line between the RFQ and the Linac in the radioactive ion beam facility at VECC, Kolkata

    Indian Academy of Sciences (India)

    S Dechoudhury; Vaishali Naik; Manas Mondal; Hemendra Kumar Pandey; Avik Chatterjee; Dirtha Sanyal; Debasis Bhowmick; Alok Chakrabarti

    2010-09-01

    The design of a medium energy beam transport (MEBT) line comprising of a re-buncher and four quadrupoles, two upstream and the other two downstream of the re-buncher, has been presented. The design was done to ensure almost 100% transport of heavy-ion beams of about 99 keV/u energy from RFQ having a / not less than 1/14 through the re-buncher and then through IH Linac of about 0.6 m length in which beam would be accelerated to about 185 keV/u. The re-buncher has been designed to operate at 37.8 MHz, the resonating frequency of both the RFQ and the IH Linac. The entire beam line has been installed and recently O5+ beam from RFQ has been transported through the re-buncher and subsequently accelerated in the IH Linac successfully.

  11. Overview of charged-particle beam diagnostics for the advanced photon source (APS)

    Science.gov (United States)

    Lumpkin, A. H.; Decker, G.; Kahana, E.; Patterson, D.; Sellyey, W.; Votaw, A.; Wang, X.; Chung, Y.

    1992-07-01

    Plans, prototypes, and initial test results for the charged-particle beam (e-,e+) diagnostic systems on the injector rings, their transport lines, and the storage ring for the Advanced Photon Source (APS) are presented. The APS will be a synchrotron radiation user facility with one of the world's brightest x-ray sources in the 10-keV to 100-keV regime. Its 200-MeV electron linac, 450-MeV positron linac, positron accumulator ring, 7-GeV booster synchrotron, 7-GeV storage ring, and undulator test lines will also demand the development and demonstration of key particle-beam characterization techniques over a wide range of parameter space. Some of these parameter values overlap or approach those projected for fourth generation light sources (linac-driven FELs and high brightness storage rings) as described at a recent workshop. Initial results from the diagnostics prototypes on the linac test stand operating at 45-MeV include current monitor data, beam loss monitor data, and video digitization using VME architecture.

  12. Charged-particle beam diagnostics for the Advanced Photon Source (APS)

    Science.gov (United States)

    Lumpkin, A. H.; Decker, G.; Kahana, E.; Patterson, D.; Sellyey, W.; Wang, X.; Chung, Y.

    1992-08-01

    Plans, prototypes, and initial test results for the charged-particle beam (e-), e(+) diagnostic systems on the injector rings, their transport lines, and the storage ring for the Advanced Photon Source (APS) are presented. The APS will be a synchrotron radiation user facility with one of the world's brightest x-ray sources in the 10-keV to 100-keV regime. Its 200-MeV electron linac, 450-MeV positron linac, positron accumulator ring, 7-GeV booster synchrotron, 7-GeV storage ring, and undulator test lines will also demand the development and demonstration of key particle-beam characterization techniques over a wide range of parameter space. Some of these parameter values overlap or approach those projected for fourth generation light sources (linac-driven FELs and high brightness storage rings) as described at a recent workshop. Initial results from the diagnostics prototypes on the linac test stand operating at 45-MeV include current monitor data, beam loss monitor data, and video digitization using VME architecture.

  13. AP Geography, Environmental Science Thrive

    Science.gov (United States)

    Robelen, Erik W.

    2012-01-01

    Geography may not be particularly known as a hot topic among today's students--even some advocates suggest it suffers from an image problem--but by at least one measure, the subject is starting to come into its own. Across more than 30 topics covered in the Advanced Placement (AP) program, participation in geography is rising faster than any…

  14. Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes.

    Science.gov (United States)

    Tolvanen, Tuomas A; Dash, Surjya Narayan; Polianskyte-Prause, Zydrune; Dumont, Vincent; Lehtonen, Sanna

    2015-12-15

    The adapter protein CD2-associated protein (CD2AP) functions in various signaling and vesicle trafficking pathways, including endosomal sorting and/or trafficking and degradation pathways. Here, we investigated the role of CD2AP in insulin-dependent glucose transporter 4 (Glut4, also known as SLC2A4) trafficking and glucose uptake. Glucose uptake was attenuated in CD2AP(-/-) podocytes compared with wild-type podocytes in the basal state, and CD2AP(-/-) podocytes failed to increase glucose uptake in response to insulin. Live-cell imaging revealed dynamic trafficking of HA-Glut4-GFP in wild-type podocytes, whereas in CD2AP(-/-) podocytes, HA-Glut4-GFP clustered perinuclearly. In subcellular membrane fractionations, CD2AP co-fractionated with Glut4, IRAP (also known as LNPEP) and sortilin, constituents of Glut4 storage vesicles (GSVs). We further found that CD2AP forms a complex with GGA2, a clathrin adaptor, which sorts Glut4 to GSVs, suggesting a role for CD2AP in this process. We also found that CD2AP forms a complex with clathrin and connects clathrin to actin in the perinuclear region. Furthermore, clathrin recycling back to trans-Golgi membranes from the vesicular fraction containing GSVs was defective in the absence of CD2AP. This leads to reduced insulin-stimulated trafficking of GSVs and attenuated glucose uptake into CD2AP(-/-) podocytes.

  15. Constitutively internalized dopamine transporter is targeted to late endosomes and lysosomal degradation in heterologous cell lines and dopaminergic neurons

    DEFF Research Database (Denmark)

    Eriksen, Jacob; Madsen, Kenneth; Vægter, Christian Bjerggaard;

    (leupeptin, chloroquine, or ammonium chloride) increased the amount of transporter accumulated intracellularly over time, suggesting that constitutively endocytosed transporter was targeted to lysosomal degradation. This was further supported by expression of Tac-DAT in the immortalized dopaminergic cell...

  16. The Electronic ApJ in Context

    Science.gov (United States)

    Biemesderfer, C. D.

    1995-12-01

    The on-line edition of the Astrophysical Journal provides the first opportunity for astronomical researchers to interact with the literature in a network context. Other electronic journals will follow. Astronomers will continue to develop ways to take advantage of information on the network, and these will be most effective when ApJ articles, observations from data centers, proposals for telescope time, communications with collaborators, and so on, are meshed. Astronomical literature, as a resource on the Internet, is not unique from the viewpoint of function or management, and astronomers are not unique as scholars in their desire to use the Internet as a tool to advance their work. We need to ensure as a community that all our resources for research --- journals and data --- fit in a global context on an Internet being used by everyone. In other words, we have to be sure that the ApJ fits into all the digital libraries of the future, including the ones we don't build. This paper will present a scenario in which many varied forms of information, astronomical and otherwise, can be catalogued and distributed so that they can be discovered and used by the research community.

  17. Low Earth orbit assessment of proton anisotropy using AP8 and AP9 trapped proton models

    Science.gov (United States)

    Badavi, Francis F.; Walker, Steven A.; Santos Koos, Lindsey M.

    2015-04-01

    The completion of the International Space Station (ISS) in 2011 has provided the space research community with an ideal evaluation and testing facility for future long duration human activities in space. Ionized and secondary neutral particles radiation measurements inside ISS form the ideal tool for validation of radiation environmental models, nuclear reaction cross sections and transport codes. Studies using thermo-luminescent detectors (TLD), tissue equivalent proportional counter (TPEC), and computer aided design (CAD) models of early ISS configurations confirmed that, as input, computational dosimetry at low Earth orbit (LEO) requires an environmental model with directional (anisotropic) capability to properly describe the exposure of trapped protons within ISS. At LEO, ISS encounters exposure from trapped electrons, protons and geomagnetically attenuated galactic cosmic rays (GCR). For short duration studies at LEO, one can ignore trapped electrons and ever present GCR exposure contributions during quiet times. However, within the trapped proton field, a challenge arises from properly estimating the amount of proton exposure acquired. There exist a number of models to define the intensity of trapped particles. Among the established trapped models are the historic AE8/AP8, dating back to the 1980s and the recently released AE9/AP9/SPM. Since at LEO electrons have minimal exposure contribution to ISS, this work ignores the AE8 and AE9 components of the models and couples a measurement derived anisotropic trapped proton formalism to omnidirectional output from the AP8 and AP9 models, allowing the assessment of the differences between the two proton models. The assessment is done at a target point within the ISS-11A configuration (circa 2003) crew quarter (CQ) of Russian Zvezda service module (SM), during its ascending and descending nodes passes through the south Atlantic anomaly (SAA). The anisotropic formalism incorporates the contributions of proton narrow

  18. Low Earth orbit assessment of proton anisotropy using AP8 and AP9 trapped proton models.

    Science.gov (United States)

    Badavi, Francis F; Walker, Steven A; Santos Koos, Lindsey M

    2015-04-01

    The completion of the International Space Station (ISS) in 2011 has provided the space research community with an ideal evaluation and testing facility for future long duration human activities in space. Ionized and secondary neutral particles radiation measurements inside ISS form the ideal tool for validation of radiation environmental models, nuclear reaction cross sections and transport codes. Studies using thermo-luminescent detectors (TLD), tissue equivalent proportional counter (TPEC), and computer aided design (CAD) models of early ISS configurations confirmed that, as input, computational dosimetry at low Earth orbit (LEO) requires an environmental model with directional (anisotropic) capability to properly describe the exposure of trapped protons within ISS. At LEO, ISS encounters exposure from trapped electrons, protons and geomagnetically attenuated galactic cosmic rays (GCR). For short duration studies at LEO, one can ignore trapped electrons and ever present GCR exposure contributions during quiet times. However, within the trapped proton field, a challenge arises from properly estimating the amount of proton exposure acquired. There exist a number of models to define the intensity of trapped particles. Among the established trapped models are the historic AE8/AP8, dating back to the 1980s and the recently released AE9/AP9/SPM. Since at LEO electrons have minimal exposure contribution to ISS, this work ignores the AE8 and AE9 components of the models and couples a measurement derived anisotropic trapped proton formalism to omnidirectional output from the AP8 and AP9 models, allowing the assessment of the differences between the two proton models. The assessment is done at a target point within the ISS-11A configuration (circa 2003) crew quarter (CQ) of Russian Zvezda service module (SM), during its ascending and descending nodes passes through the south Atlantic anomaly (SAA). The anisotropic formalism incorporates the contributions of proton narrow

  19. Time, Concentration, and pH-Dependent Transport and Uptake of Anthocyanins in a Human Gastric Epithelial (NCI-N87) Cell Line

    Science.gov (United States)

    Atnip, Allison A.; Sigurdson, Gregory T.; Bomser, Joshua; Giusti, M. Mónica

    2017-01-01

    Anthocyanins are the largest class of water soluble plant pigments and a common part of the human diet. They may have many potential health benefits, including antioxidant, anti-inflammatory, anti-cancer, and cardioprotective activities. However, anthocyanin metabolism is not well understood. Studies suggest that anthocyanins absorption may occur in the stomach, in which the acidic pH favors anthocyanin stability. A gastric epithelial cell line (NCI-N87) has been used to study the behavior of anthocyanins at a pH range of 3.0–7.4. This work examines the effects of time (0–3 h), concentration (50–1500 µM), and pH (3.0, 5.0, 7.4) on the transport and uptake of anthocyanins using NCI-N87 cells. Anthocyanins were transported from the apical to basolateral side of NCI-N87 cells in time and dose dependent manners. Over the treatment time of 3 h the rate of transport increased, especially with higher anthocyanin concentrations. The non-linear rate of transport may suggest an active mechanism for the transport of anthocyanins across the NCI-N87 monolayer. At apical pH 3.0, higher anthocyanin transport was observed compared to pH 5.0 and 7.4. Reduced transport of anthocyanins was found to occur at apical pH 5.0. PMID:28218720

  20. Time, Concentration, and pH-Dependent Transport and Uptake of Anthocyanins in a Human Gastric Epithelial (NCI-N87 Cell Line

    Directory of Open Access Journals (Sweden)

    Allison A. Atnip

    2017-02-01

    Full Text Available Anthocyanins are the largest class of water soluble plant pigments and a common part of the human diet. They may have many potential health benefits, including antioxidant, anti-inflammatory, anti-cancer, and cardioprotective activities. However, anthocyanin metabolism is not well understood. Studies suggest that anthocyanins absorption may occur in the stomach, in which the acidic pH favors anthocyanin stability. A gastric epithelial cell line (NCI-N87 has been used to study the behavior of anthocyanins at a pH range of 3.0–7.4. This work examines the effects of time (0–3 h, concentration (50–1500 µM, and pH (3.0, 5.0, 7.4 on the transport and uptake of anthocyanins using NCI-N87 cells. Anthocyanins were transported from the apical to basolateral side of NCI-N87 cells in time and dose dependent manners. Over the treatment time of 3 h the rate of transport increased, especially with higher anthocyanin concentrations. The non-linear rate of transport may suggest an active mechanism for the transport of anthocyanins across the NCI-N87 monolayer. At apical pH 3.0, higher anthocyanin transport was observed compared to pH 5.0 and 7.4. Reduced transport of anthocyanins was found to occur at apical pH 5.0.

  1. Space-charge compensation measurements in electron cyclotron resonance ion source low energy beam transport lines with a retarding field analyzer

    Energy Technology Data Exchange (ETDEWEB)

    Winklehner, D.; Leitner, D., E-mail: leitnerd@nscl.msu.edu; Cole, D.; Machicoane, G.; Tobos, L. [National Superconducting Cyclotron Laboratory, Michigan State University, East Lansing, Michigan 48824 (United States)

    2014-02-15

    In this paper we describe the first systematic measurement of beam neutralization (space charge compensation) in the ECR low energy transport line with a retarding field analyzer, which can be used to measure the potential of the beam. Expected trends for the space charge compensation levels such as increase with residual gas pressure, beam current, and beam density could be observed. However, the overall levels of neutralization are consistently low (<60%). The results and the processes involved for neutralizing ion beams are discussed for conditions typical for ECR injector beam lines. The results are compared to a simple theoretical beam plasma model as well as simulations.

  2. Overexpression of ligase defective E6-associated protein, E6-AP, results in mammary tumorigenesis.

    Science.gov (United States)

    Ramamoorthy, Sivapriya; Tufail, Rozina; Hokayem, Jimmy El; Jorda, Mercy; Zhao, Wei; Reis, Zizi; Nawaz, Zafar

    2012-02-01

    E6-associated protein (E6-AP) is a dual function protein. It acts as an E3 ubiquitin-protein ligase enzyme and coactivator of steroid hormone receptors such as estrogen (ERα) and progesterone (PR) receptors. It promotes the degradation of ERα and PR through the ubiquitin-proteasome pathway. Furthermore, it has been shown that the levels of E6-AP are inversely associated with that of ERα in human breast tumors. But the role of wild-type human E6-AP and its ubiquitin-protein ligase activity in mammary tumorigenesis is still unknown. To investigate this role, the authors utilized transgenic mice lines that specifically overexpress either the wild-type human E6-AP (E6-AP(WT)) or the ubiquitin-protein ligase defective E6-AP that contains C833S mutation (E6-AP(C833S)) in the mammary gland. To further substantiate the role of E6-AP in the development of breast tumorigenesis, it was also examined the expression of E6-AP in a large cohort of human breast cancer samples. The transgenic mice that overexpress wild-type E6-AP (E6-AP(WT)) fail to develop mammary tumors. Unlike the E6-AP(WT) mice, the E6-AP(C833S) mice that overexpress ubiquitin-protein ligase defective E6-AP protein develop mammary hyperplasia with a median latency of 18 months. These observations suggest that the inactivation of the ubiquitin-protein ligase function of E6-AP is sufficient to initiate the process of mammary tumor development. Furthermore, the data also suggests that E6-AP exerts its effects on target cells by modulating the protein levels and functions of ERα and PR. In addition, it was found in human breast cancer patients that the level of E6-AP is decreased in invasive breast tumors compared to normal breast tissue. Moreover, the authors also show that the survival patterns for E6-AP negative patients were worse compared to E6-AP positive patients. Taken together, these data suggests that E6-AP may act as a tumor suppressor in breast.

  3. Design of a medium-energy beam-transport line with an anti-chopper for the JAERI/KEK project

    CERN Document Server

    Wang Sheng; Kato, T

    2002-01-01

    The medium-energy beam-transport line (MEBT) plays an important role in reducing beam loss in the JAERI/KEK project. A MEBT was designed two years ago, with good beam matching and lower beam loss. To further reduce beam loss during the transient time of the chopper to meet the new requirement from the DTL, a medium-energy beam-transport line with an anti-chopper has been designed. The 3.5 m long transport line consists of nine quadrupole magnets, three bunchers and four chopper/anti-chopper cavities. It accomplishes two tasks: matching the beam from the RFQ to the acceptance of the DTL and chopping the beam to produce gaps for injection into the rapid-cycling ring, which follows the linac. A RF Chopper and an anti-chopper have been adopted in the lattice, resulting in a clean chopping effect and no beam losses during the transient time. Details of the beam dynamics analysis are given

  4. The Role of the Clathrin Adaptor AP-1: Polarized Sorting and Beyond

    Directory of Open Access Journals (Sweden)

    Fubito Nakatsu

    2014-11-01

    Full Text Available The selective transport of proteins or lipids by vesicular transport is a fundamental process supporting cellular physiology. The budding process involves cargo sorting and vesicle formation at the donor membrane and constitutes an important process in vesicular transport. This process is particularly important for the polarized sorting in epithelial cells, in which the cargo molecules need to be selectively sorted and transported to two distinct destinations, the apical or basolateral plasma membrane. Adaptor protein (AP-1, a member of the AP complex family, which includes the ubiquitously expressed AP-1A and the epithelium-specific AP-1B, regulates polarized sorting at the trans-Golgi network and/or at the recycling endosomes. A growing body of evidence, especially from studies using model organisms and animals, demonstrates that the AP-1-mediated polarized sorting supports the development and physiology of multi-cellular units as functional organs and tissues (e.g., cell fate determination, inflammation and gut immune homeostasis. Furthermore, a possible involvement of AP-1B in the pathogenesis of human diseases, such as Crohn’s disease and cancer, is now becoming evident. These data highlight the significant contribution of AP-1 complexes to the physiology of multicellular organisms, as master regulators of polarized sorting in epithelial cells.

  5. Transport inhibition of digoxin using several common P-gp expressing cell lines is not necessarily reporting only on inhibitor binding to P-gp.

    Directory of Open Access Journals (Sweden)

    Annie Albin Lumen

    Full Text Available We have reported that the P-gp substrate digoxin required basolateral and apical uptake transport in excess of that allowed by digoxin passive permeability (as measured in the presence of GF120918 to achieve the observed efflux kinetics across MDCK-MDR1-NKI (The Netherlands Cancer Institute confluent cell monolayers. That is, GF120918 inhibitable uptake transport was kinetically required. Therefore, IC50 measurements using digoxin as a probe substrate in this cell line could be due to inhibition of P-gp, of digoxin uptake transport, or both. This kinetic analysis is now extended to include three additional cell lines: MDCK-MDR1-NIH (National Institute of Health, Caco-2 and CPT-B2 (Caco-2 cells with BCRP knockdown. These cells similarly exhibit GF120918 inhibitable uptake transport of digoxin. We demonstrate that inhibition of digoxin transport across these cell lines by GF120918, cyclosporine, ketoconazole and verapamil is greater than can be explained by inhibition of P-gp alone. We examined three hypotheses for this non-P-gp inhibition. The inhibitors can: (1 bind to a basolateral digoxin uptake transporter, thereby inhibiting digoxin's cellular uptake; (2 partition into the basolateral membrane and directly reduce membrane permeability; (3 aggregate with digoxin in the donor chamber, thereby reducing the free concentration of digoxin, with concomitant reduction in digoxin uptake. Data and simulations show that hypothesis 1 was found to be uniformly acceptable. Hypothesis 2 was found to be uniformly unlikely. Hypothesis 3 was unlikely for GF120918 and cyclosporine, but further studies are needed to completely adjudicate whether hetero-dimerization contributes to the non-P-gp inhibition for ketoconazole and verapamil. We also find that P-gp substrates with relatively low passive permeability such as digoxin, loperamide and vinblastine kinetically require basolateral uptake transport over that allowed by +GF120918 passive permeability, while

  6. Molecular cloning of two Arabidopsis UDP-galactose transporters by complementation of a deficient Chinese hamster ovary cell line

    NARCIS (Netherlands)

    Bakker, H.; Routier, F.; Oelmann, S.; Jordi, W.J.R.M.; Lommen, A.; Gerardy-Schahn, R.; Bosch, H.J.

    2005-01-01

    Nucleotide-sugar transporters (NSTs) form a family of structurally related transmembrane proteins that transport nucleotide-sugars from the cytoplasm to the endoplasmic reticulum and Golgi lumen. In these organelles, activated sugars are substrates for various glycosyltransferases involved in oligo-

  7. An AP Calculus Classroom Amusement Park

    Science.gov (United States)

    Ferguson, Sarah

    2016-01-01

    Throughout the school year, AP Calculus teachers strive to teach course content comprehensively and swiftly in an effort to finish all required material before the AP Calculus exam. As early May approaches and the AP Calculus test looms, students and teachers nervously complete lessons, assignments, and assessments to ensure student preparation.…

  8. Geant4 simulations of proton beam transport through a carbon or beryllium degrader and following a beam line

    NARCIS (Netherlands)

    van Goethem, M. J.; van der Meer, R.; Reist, H. W.; Schippers, J. M.

    2009-01-01

    Monte Carlo simulations based on the Geant4 simulation toolkit were performed for the carbon wedge degrader used in the beam line at the Center of Proton Therapy of the Paul Scherrer Institute (PSI). The simulations are part of the beam line studies for the development and understanding of the GANTR

  9. Advantages of electrical propulsion applied to the urban transportation with express lines; Vantagens da propulsao eletrica aplicada ao transporte urbano em linhas expressas

    Energy Technology Data Exchange (ETDEWEB)

    Schwob, Marcelo Rousseau Valenca; Rodrigues, Joaquim Augusto Pinto; Henriques Junior, Mauricio F.; Dresch, Patricia Miranda; Dantas, Fabricio dos Santos [Instituto Nacional de Tecnologia (INT), Rio de Janeiro, RJ (Brazil). Div. de Energia], E-mail: energia@int.gov.br; Bernardes, Fernanda Manhaes [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil)

    2010-07-01

    This paper intends to compare the various possible solutions for urban bus propulsion under the environmental aspects (gaseous emissions, vibrations and noises), energy efficiency and energetic operational costs, facing the evident signals of energy inefficiency and environmental limitations presented at urban highway transports of large cities.

  10. Fellow's Apéro

    CERN Multimedia

    Staff Association

    2017-01-01

    Let's get together, meet each other, exchange experiences and ideas, and share useful information on CERN and the Staff Association. Join us for Fellow's Apéro, organised by the Staff Association on Tuesday 21 February at 16.30 in Restaurant 1. There will be drinks and snacks for everybody! We look forward to seeing you there! Please confirm your participation on Doodle http://doodle.com/poll/skvm7ucm2z78i6bt or alternatively on Facebook https://www.facebook.com/events/1862757017340069/. Your delegates in the Staff Association, Barbora & Jiri

  11. Testing of Solar Heated Domestic Hot Water System for Solahart Scandinavia ApS

    DEFF Research Database (Denmark)

    Andersen, Elsa

    1997-01-01

    The solar heating system marketed by Solahart Scandinavia ApS was tested in the Institutes test facility for SDHWsystems. The test results are described in the report.......The solar heating system marketed by Solahart Scandinavia ApS was tested in the Institutes test facility for SDHWsystems. The test results are described in the report....

  12. Ectopic expression of Jatropha curcas APETALA1 (JcAP1 caused early flowering in Arabidopsis, but not in Jatropha

    Directory of Open Access Journals (Sweden)

    Mingyong Tang

    2016-04-01

    Full Text Available Jatropha curcas is a promising feedstock for biofuel production because Jatropha oil is highly suitable for the production of biodiesel and bio-jet fuels. However, Jatropha exhibits a low seed yield as a result of unreliable and poor flowering. APETALA1 (AP1 is a floral meristem and organ identity gene in higher plants. The flower meristem identity genes of Jatropha have not yet been identified or characterized. To better understand the genetic control of flowering in Jatropha, an AP1 homolog (JcAP1 was isolated from Jatropha. An amino acid sequence analysis of JcAP1 revealed a high similarity to the AP1 proteins of other perennial plants. JcAP1 was expressed in inflorescence buds, flower buds, sepals and petals. The highest expression level was observed during the early developmental stage of the flower buds. The overexpression of JcAP1 using the cauliflower mosaic virus (CaMV 35S promoter resulted in extremely early flowering and abnormal flowers in transgenic Arabidopsis plants. Several flowering genes downstream of AP1 were up-regulated in the JcAP1-overexpressing transgenic plant lines. Furthermore, JcAP1 overexpression rescued the phenotype caused by the Arabidopsis AP1 loss-of-function mutant ap1-11. Therefore, JcAP1 is an ortholog of AtAP1, which plays a similar role in the regulation of flowering in Arabidopsis. However, the overexpression of JcAP1 in Jatropha using the same promoter resulted in little variation in the flowering time and floral organs, indicating that JcAP1 may be insufficient to regulate flowering by itself in Jatropha. This study helps to elucidate the function of JcAP1 and contributes to the understanding of the molecular mechanisms of flower development in Jatropha.

  13. Railroad Lines - RAIL_CROSSINGS_INDOT_IN: Railroad Crossings in Indiana (Indiana Department of Transportation, 1:1200, Point Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — RAIL_CROSSINGS_INDOT_IN is a point shapefile that contains locations of rail crossings in Indiana, provided by personnel of Indiana Department of Transportation...

  14. Railroad Lines, Rail Corridors, Published in 2014, 1:2400 (1in=200ft) scale, Madison Area Transportation Planning Board.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Railroad Lines dataset, published at 1:2400 (1in=200ft) scale, was produced all or in part from Orthoimagery information as of 2014. It is described as 'Rail...

  15. Vision document on transport scarcity. Policy line for shortage in transport capacity on the electricity grid; Visiedocument transportschaarste. Beleidslijn inzake de schaarste aan transportcapaciteit op het elektriciteitsnetwerk

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-01-15

    The Netherlands Competition Authority (NMa) has observed an increasing problem in relation to possible future shortage of transport capacity in the electricity grid. This vision document aims to provide clarity to market parties with regard to their general vision on this shortage problem [mk]. [Dutch] De Nederlandse Mededingingsautoriteit (NMa) heeft geconstateerd dat er in toenemende mate problemen optreden die verband houden met de (mogelijk toekomstige) schaarste aan transportcapaciteit op het elektriciteitsnetwerk.Met dit Visiedocument beoogt de NMa zoveel mogelijk duidelijkheid te verschaffen aan marktpartijen met betrekking tot zijn algemene visie ten aanzien van de schaarsteproblematiek.

  16. Berberine inhibits cyclin D1 expression via suppressed binding of AP-1 transcription factors to CCND1 AP-1 motif

    Institute of Scientific and Technical Information of China (English)

    Ye LUO; Yu HAO; Tai-ping SHI; Wei-wei DENG; Na LI

    2008-01-01

    Aim: To verify the suppressive effect of berberine on the proliferation of the human pulmonary giant cell carcinoma cell line PG and to demonstrate the mecha-nisms behind the antitumoral effects of berberine. Methods: The proliferative effects of PG cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetry. The cell cycle was examined by flow cytometry. The expression level of cyclin D1 was detected by RT-PCR. The activities of the activating protein-1 (AP-1) and NF-κB signaling pathways related to cyclin D1 were examined by luciferase assay. The cytoplasmic level of c-Jun was detected by Western blot analysis. An electrophoretic mobility shift assay was used to examiae the binding of transcription factors to the cyclin D1 gene (CCNDl) AP-1 motif. Results: The results showed that the proliferation of PG cells treated with different concentrations (10, 20, and 40 μg/mL) of berberine for 24 and 48 h was suppressed significantly compared to the control group. After treatment with berberine, the proportion of PG cells at the G0/G1 phase increased, while cells at the S and G2/M phases decreased. Berberine could inhibit the expression of cyclin D1 in PG cells. Berberine inhibited the activity of the AP-1 signaling pathway, but had no significant effect on the NF-κB signaling pathway. Berberine suppressed the expression of c-Jun and decreased the binding of tran-scription factors to the CCND1 AP-1 motif. Conclusion: Berberine suppresses the activity of the AP-1 signaling pathway and decreases the binding of transcrip-tion factors to the CCND1 AP-1 motif. This is one of the important mechanisms behind the antitumoral effects of berberine as a regulator of cyclin D1.

  17. Design of a two-ion-source (2-IS) beam transport line for the production of multi charged radioactive ion beams

    CERN Document Server

    Banerjee, V; Bandyopadhyay, A; Chattopadhyay, S; Polley, A; Nakagawa, T; Kamigaito, O; Goto, A; Yano, Y

    2000-01-01

    A 'two-ion-source' beam transport line between a surface ionization source and a 6.4 GHz on-line Electron Cyclotron Resonance Ion Source (ECRIS) for the production of multi-charged radioactive ions has been designed. The 1 sup + ions from the surface ionization source are decelerated and focused onto the ECRIS plasma so that they can be efficiently trapped there and further ionized to charge state q>1 sup +. A scheme for stepwise and gradual deceleration of the 1 sup + ion beam consisting of a multi-electrode decelerator and a tuning electrode placed before the ECRIS has been optimized. The beam dynamics calculations show that the 1 sup + beam decelerated to energies of 20-50 eV could be focused to a spot size smaller than the radial dimensions of the ECR plasma zone.

  18. Kinetics and conductivity parameters of uptake and transport of polychlorinated biphenyls in the Caco-2 intestinal cell line model

    NARCIS (Netherlands)

    Dulfer, W.J.; Govers, H.A.J.; Groten, J.P.

    1998-01-01

    Most of the accumulation of polychlorinated biphenyls (PCBs) over the food chain can be attributed to contaminant uptake from food. The effect of fatty acid absorption on net uptake and transport fluxes of a selection of 14 PCBs over the organismal gut epithelium has been determined in monolayers of

  19. Off-line algorithm for calculation of vertical tracer transport in the troposphere due to deep convection

    Directory of Open Access Journals (Sweden)

    D. A. Belikov

    2012-08-01

    Full Text Available A modified cumulus convection parametrisation scheme is presented. This scheme computes the mass of air transported upward in a cumulus cell using conservation of moisture and a detailed distribution of convective precipitation provided by a reanalysis dataset. The representation of vertical transport within the scheme includes entrainment and detrainment processes in convective updrafts and downdrafts. Output from the proposed parametrisation scheme is employed in the National Institute for Environmental Studies (NIES global chemical transport model driven by JRA-25/JCDAS reanalysis. The simulated convective precipitation rate and mass fluxes are compared with observations and reanalysis data. A simulation of the short-lived tracer 222Rn is used to further evaluate the performance of the cumulus convection scheme. Simulated distributions of 222Rn are validated against observations at the surface and in the free troposphere, and compared with output from models that participated in the TransCom-CH4 Transport Model Intercomparison. From this comparison, we demonstrate that the proposed convective scheme can successfully reproduce deep cloud convection.

  20. Off-line algorithm for calculation of vertical tracer transport in the troposphere due to deep convection

    Directory of Open Access Journals (Sweden)

    D. A. Belikov

    2013-02-01

    Full Text Available A modified cumulus convection parametrisation scheme is presented. This scheme computes the mass of air transported upward in a cumulus cell using conservation of moisture and a detailed distribution of convective precipitation provided by a reanalysis dataset. The representation of vertical transport within the scheme includes entrainment and detrainment processes in convective updrafts and downdrafts. Output from the proposed parametrisation scheme is employed in the National Institute for Environmental Studies (NIES global chemical transport model driven by JRA-25/JCDAS reanalysis. The simulated convective precipitation rate and mass fluxes are compared with observations and reanalysis data. A simulation of the short-lived tracer 222Rn is used to further evaluate the performance of the cumulus convection scheme. Simulated distributions of 222Rn are evaluated against observations at the surface and in the free troposphere, and compared with output from models that participated in the TransCom-CH4 Transport Model Intercomparison. From this comparison, we demonstrate that the proposed convective scheme in general is consistent with observed and modeled results.

  1. Off-line algorithm for calculation of vertical tracer transport in the troposphere due to deep convection

    NARCIS (Netherlands)

    Belikov, D.A.; Maksyutov, S.; Krol, M.C.; Fraser, A.; Rigby, M.; Bian, H.; Agusti-Panareda, A.; Bergmann, D.; Bousquet, P.; Cameron-Smith, P.; Chipperfield, M.P.; Fortems-Cheiney, A.; Gloor, E.; Haynes, K.; Hess, P.; Houweling, S.; Kawa, S.R.; Law, R.M.; Loh, Z.; Meng, L.; Palmer, P.I.; Patra, P.K.; Prinn, R.G.; Saito, R.; Wilson, C.

    2013-01-01

    A modified cumulus convection parametrisation scheme is presented. This scheme computes the mass of air transported upward in a cumulus cell using conservation of moisture and a detailed distribution of convective precipitation provided by a reanalysis dataset. The representation of vertical transpo

  2. Transport characteristics of L-carnosine and the anticancer derivative 4-toluenesulfonylureido-carnosine in a human epithelial cell line

    DEFF Research Database (Denmark)

    Nielsen, Carsten Uhd; Supuran, Claudiu T; Scozzafava, Andrea;

    2002-01-01

    The aim of the present study was to evaluate whether the transepithelial transport of the anticancer compound 4-toluenesulfonylureido-carnosine (Ts-carnosine) and the dipeptide moiety L-carnosine was due to a hPepT1 carrier-mediated flux....

  3. Analysis of high iron rice lines reveals new miRNAs that target iron transporters in roots

    Science.gov (United States)

    Paul, Soumitra; Gayen, Dipak; Datta, Swapan K.; Datta, Karabi

    2016-01-01

    The present study highlights the molecular regulation of iron transport in soyFER1-overexpressing transgenic rice. Accumulation of iron in three different seed developmental stages, milk, dough, and mature, has been examined. The transgenic seeds of the milk stage showed significant augmentation of iron and zinc levels compared with wild-type seeds, and similar results were observed throughout the dough and mature stages. To investigate the regulation of iron transport, the role of miRNAs was studied in roots of transgenic rice. Sequencing of small RNA libraries revealed 153 known and 41 novel miRNAs in roots. Among them, 59 known and 14 novel miRNAs were found to be significantly expressed. miR166, miR399, and miR408 were identified as playing a vital role in iron uptake in roots of transgenic plants . Most importantly, four putative novel miRNAs, namely miR11, miR26, miR30, and miR31, were found to be down-regulated in roots of transgenic plants. For all these four novel miRNAs, natural resistance-associated macrophage protein 4 (NRAMP4), encoding a metal transporter, was predicted as a target gene. It is hypothesized that the NRAMP4 transporter is activated in roots of transgenic plants due to the lower abundance of its corresponding putative novel miRNAs. The relative transcript level of the NRAMP4 transcript was increased from 0.107 in the wild type to 65.24 and 55.39 in transgenic plants, which demonstrates the elevated amount of iron transport in transgenic plants. In addition, up-regulation of OsYSL15, OsFRO2, and OsIRT1 in roots also facilitates iron loading in transgenic seeds. PMID:27729476

  4. On AP-property of Function Spaces

    Institute of Scientific and Technical Information of China (English)

    SHEN Rong-xin

    2012-01-01

    In this paper,it is discussed the AP-property of function spaces.We prove that for any compact network α for a space X which is closed under finite unions,(1) if Cα(X) is an AP-space and X is paracompact,then X is a Hurewicz space; (2) if Cα (X) is an AP-space which has countable tightness,then Cα(X) is discretely generated.

  5. AP calculus AB & BC crash course

    CERN Document Server

    Rosebush, J

    2012-01-01

    AP Calculus AB & BC Crash Course - Gets You a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. AP Calculus AB & BC Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know Crash Course is based on an in-depth analysis of the AP Calculus AB & BC course description outline and actual AP test questions. It covers only the information tested on the exams, so you can make the most of your valuable study time. Written by experienced math teachers, our

  6. Unsaturated fatty acids and phytosterols regulate cholesterol transporter genes in Caco-2 and HepG2 cell lines.

    Science.gov (United States)

    Park, Youngki; Carr, Timothy P

    2013-02-01

    Dietary consumption of phytosterols and certain fatty acids has been shown to reduce cholesterol absorption and plasma cholesterol concentrations. However, it has not been fully elucidated whether phytosterols or fatty acids can alter the expression of cholesterol transporters by functioning as signaling molecules. This study tested the hypothesis that various fatty acids and phytosterols commonly found in the food supply can modulate the expression of transporters including Niemann-Pick C1-like 1, low-density lipoprotein receptor, and scavenger receptor class B type I and 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the intestine and liver. Caco-2 cells were used as models of enterocytes, and HepG2 cells were used as a model of hepatocytes. The cells were treated for 18 hours with 100 μmol/L of a fatty acid, or for 24 hours with 10 μmol/L of 25α-hydroxycholesterol, or 100 μmol/L of cholesterol, sitosterol, and stigmasterol to measure expression of genes involved in cholesterol transport using quantitative real-time polymerase chain reaction. Polyunsaturated fatty acids in Caco-2 cells and sterols in HepG2 cells significantly reduced the messenger RNA expression levels of Niemann-Pick C1-like 1, scavenger receptor class B type I, low-density lipoprotein receptor, and 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Importantly, sitosterol and stigmasterol reduced the messenger RNA levels of genes to a similar extent as cholesterol. The data support the hypothesis that unsaturated fatty acid and phytosterols can act as signaling molecules and alter the expression of genes involved in cholesterol transport and metabolism.

  7. An Optimization Design of Comprehensive Passenger Transport Hubs Linking Bus Lines%综合客运枢纽接运公交线路优化设计

    Institute of Scientific and Technical Information of China (English)

    王佳; 符卓

    2012-01-01

    The intrinsic value and role are analyzed on the optimization design of comprehensive passenger transport hub linking bus lines. An optimization model with two aims -- maximum transportation efficiency and minimum bus lines -- is established and converted to an open vehicle routing problem with pickup and delivery for solution. The tabu search algorithm for solution is given in this paper and a case is studied to verify algorithm. It is proved that the algorithm performs a good optimization-searching function.%分析了综合客运枢纽接运公交线路优化设计问题的内涵及作用,构建了以运输效率最大和布设线路最小为双目标的优化模型,并将它转化为一类特殊的取送一体化的开放式车辆路径问题进行求解,给出了求解的禁忌搜索算法。最后通过案例进行了验证,证实了该算法具有良好的寻优性能。

  8. AP-2δ is a crucial transcriptional regulator of the posterior midbrain.

    Directory of Open Access Journals (Sweden)

    Katrin Hesse

    Full Text Available Ap-2 transcription factors comprise a family of 5 closely related sequence-specific DNA binding proteins that play pivotal and non-redundant roles in embryonic organogenesis. To investigate the function of Ap-2δ, wδe analyzed its expression during embryogenesis and generated Ap-2δ-deficient mice. In line with the specific expression pattern of Ap-2δ in the mesencephalic tectum and the dorsal midbrain, Ap-2δ-deficient mice failed to maintain the colliculus inferior, a derivative of the dorsal midbrain, as a consequence of increased apoptotic cell death. To identify specific Ap-2δ target genes in cells of the developing dorsal midbrain, we performed whole genome analysis of cDNA expression levels. This approach identified a set of 12 putative target genes being expressed in the developing midbrain, including the transcription factors Pitx2, Mef2c, Bhlhb4 and Pou4f3. Using chromatin immunoprecipitation (CHIP we showed that some of these genes are direct targets of Ap-2δ. Consistently, we demonstrate that Ap-2δ occupies and activates the Pou4f3 and Bhlhb4 promoters. In addition, known Pou4f3 target genes were downregulated in the posterior midbrain of Ap-2δ-deficient mice. Despite the absence of a central part of the auditory pathway, the presence of neuronal responses to sounds in the neocortex of Ap-2δ-deficient mice indicates that auditory information from the brainstem still reaches the neocortex. In summary, our data define Ap-2δ as an important transcription factor, specifying gene expression patterns required for the development of the posterior midbrain.

  9. Regulation of glucose transport in the NIH 3T3 L1 preadipocyte cell line by TCDD.

    OpenAIRE

    1994-01-01

    This study examined the changes in cellular glucose uptake induced by 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) as measured by quantification of intracellular radioactivity in the NIH 3T3 L1 preadipocyte cell line after a 30-minute incubation with the non-metabolizable radioactive analogue of glucose, 3-O-methyl-D-[1-3H] glucose. Treatment of differentiated NIH 3T3 L1 cells with TCDD produced a time- and dose-dependent decrease in the cellular uptake of glucose. Treatment of cells for 3 hr w...

  10. Development of Superconducting Combined Function Magnets for the Proton Transport Line for the J-PARC Neutrino Experiments

    CERN Document Server

    Nakamoto, Tatsushi; Anerella, Michael; Escallier, John; Fujii, T; Fukui, Yuji; Ganetis, George; Gupta, Ramesh C; Harrison, Michael; Hashiguchi, E; Higashi, Norio; Ichikawa, Atsuko; Iwamoto, Yosuke; Jain, Animesh K; Kanahara, T; Kimura, Nobuhiro; Kobayashi, Takashi; Makida, Yasuhiro; Muratore, Joseph F; Obana, Tetsuhiro; Ogitsu, T; Ohhata, Hirokatsu; Okamura, T; Orikasa, T; Parker, Brett; Sasaki, Ken Ichi; Takasaki, Minoru; Tanaka, Ken Ichi; Terashima, Akio; Tomaru, Takayuki; Wanderer, Peter; Yamamoto, Akira

    2005-01-01

    A second generation of long-baseline neutrino oscillation experiments has been proposed as one of the main projects at J-PARC jointly built by JAERI and KEK. Superconducting combined function magnets, SCFMs, will be utilized for the 50 GeV, 750 kW proton beam line for the neutrino experiment and an R&D program is in underway at KEK. The magnet is designed to provide a combined function of a dipole field of 2.6 T with a quadrupole field of 19 T/m in a coil aperture of 173.4 mm. A series of 28 magnets in the beam line will be operated DC in supercritical helium cooling below 5 K. A design feature of the SCFM is the left-right asymmetry of the coil cross section: current distributions for superimposed dipole- and quadrupole- fields are combined in a single layer coil. Another design feature is the adoption of glass-fiber reinforced phenolic plastic spacers to replace the conventional metallic collars. To evaluate this unique design, fabrication of full-scale prototype magnets is in progress at KEK and the fi...

  11. The cell line NCl-H441 is a useful in vitro model for transport studies of human distal lung epithelial barrier.

    Science.gov (United States)

    Salomon, Johanna J; Muchitsch, Viktoria E; Gausterer, Julia C; Schwagerus, Elena; Huwer, Hanno; Daum, Nicole; Lehr, Claus-Michael; Ehrhardt, Carsten

    2014-03-03

    The lack of a well characterized, continuously growing in vitro model of human distal lung epithelial phenotype constitutes a serious limitation in the area of inhalation biopharmaceutics, particularly in the context of transepithelial transport studies. Here, we investigated if a human lung adenocarcinoma cell line, NCl-H441, has potential to serve as an in vitro model of human distal lung epithelium. The development of barrier properties was studied by immunocytochemistry (ICC) against the junction proteins zonula occludens protein 1 (ZO-1) and E-cadherin and measurement of transepithelial electrical resistance (TEER). Moreover, transport studies with the paracellular marker compounds fluorescein sodium and fluorescein isothiocyanate (FITC)-labeled dextrans of molecular weights ranging from 4 to 70 kDa were carried out. The expression of P-glycoprotein (P-gp; ABCB1) and organic cation transporters (OCT/Ns; SLC22A1-A5) was investigated by ICC and immunoblot. P-gp function was assessed by monolayer release and bidirectional transport studies using rhodamine 123 (Rh123) and the inhibitors verapamil and LY335979. Uptake of 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP(+)) was measured, in order to assess organic cation transporter function in vitro. Furthermore, the inhibitory potential of several organic cations on ASP(+) uptake was studied. NCl-H441 cells, when grown under liquid-covered conditions, formed confluent, electrically tight monolayers with peak TEER values of approximately 1000 Ω·cm(2), after 8-12 days in culture. These monolayers were able to differentiate paracellularly transported substrates according to their molecular weight. Presence of P-gp, OCT1, OCT2, OCT3, OCTN1, and OCTN2 was confirmed by Western blot and ICC and was similar to data from freshly isolated human alveolar epithelial cells in primary culture. Rh123 release from NCI-H441 monolayers was time-dependent and showed low, albeit significant attenuation by both inhibitors

  12. A novel dopamine transporter transgenic mouse line for identification and purification of midbrain dopaminergic neurons reveals midbrain heterogeneity

    DEFF Research Database (Denmark)

    Christiansen, Mia Apuschkin; Stilling, Sara; Rahbek-Clemmensen, Troels;

    2015-01-01

    Midbrain dopaminergic (DAergic) neurons are a heterogeneous cell group, composed of functionally distinct cell populations projecting to the basal ganglia, prefrontal cortex and limbic system. Despite their functional significance, the midbrain population of DAergic neurons is sparse, constituting...... of the dopamine transporter (DAT) promoter was characterized. Confocal microscopy analysis of brain sections showed strong eGFP signal reporter in midbrain regions and striatal terminals that co-localized with the DAergic markers DAT and tyrosine hydroxylase (TH). Thorough quantification of co......-localization of the eGFP reporter signal with DAT and TH in the ventral midbrain showed that a vast majority of eGFP-expressing neurons are DAergic. Importantly, expression profiles also revealed DAergic heterogeneity when comparing substantia nigra and ventral tegmental area. Dat1-eGFP mice showed neither change...

  13. Auxin Transport and Ribosome Biogenesis Mutant/Reporter Lines to Study Plant Cell Growth and Proliferation under Altered Gravity

    Science.gov (United States)

    Valbuena, Miguel A.; Manzano, Ana I.; van Loon, Jack JWA.; Saez-Vasquez, Julio; Carnero-Diaz, Eugenie; Herranz, Raul; Medina, F. J.

    2013-02-01

    We tested different Arabidopsis thaliana strains to check their availability for space use in the International Space Station (ISS). We used mutants and reporter gene strains affecting factors of cell proliferation and cell growth, to check variations induced by an altered gravity vector. Seedlings were grown either in a Random Positioning Machine (RPM), under simulated microgravity (μg), or in a Large Diameter Centrifuge (LDC), under hypergravity (2g). A combination of the two devices (μgRPM+LDC) was also used. Under all gravity alterations, seedling roots were longer than in control 1g conditions, while the levels of the nucleolar protein nucleolin were depleted. Alterations in the pattern of expression of PIN2, an auxin transporter, and of cyclin B1, a cell cycle regulator, were shown. All these alterations are compatible with previous space data, so the use of these strains will be useful in the next experiments in ISS, under real microgravity.

  14. Inhibition of ALDH1A1 activity decreases expression of drug transporters and reduces chemotherapy resistance in ovarian cancer cell lines.

    Science.gov (United States)

    Januchowski, Radosław; Wojtowicz, Karolina; Sterzyńska, Karolina; Sosińska, Patrycja; Andrzejewska, Małgorzata; Zawierucha, Piotr; Nowicki, Michał; Zabel, Maciej

    2016-09-01

    The high mortality of ovarian cancer patients results from the failure of treatment caused by the inherent or acquired chemotherapy drug resistance. It was reported that overexpression of aldehyde dehydrogenase A1 (ALDH1A1) in cancer cells can be responsible for the development of drug resistance. To add the high expression of the drug transporter proteins the ALDHA1 is considered as a molecular target in cancer therapy. Therefore, we analysed drug-resistant ovarian cancer cell lines according to ALDHA1 expression and the association with drug resistance. The expression of ALDH1A1, P-glycoprotein (P-gp) or breast cancer resistance protein (BCRP) was determined using a microarray and confirmed by Q-PCR, western blot and fluorescence analysis. ALDH1A1 activity was determined using an Aldefluor assay. The impact of all-trans retinoic acid (ATRA) and diethylaminobenzaldehyde (DEAB) on chemotherapy resistance was assessed by the MTT chemosensitivity assay. The most abundant expression of ALDH1A1 was noted in paclitaxel- and topotecan-resistant cell lines where two populations of ALDH-positive and ALDH-negative cells could be observed. Those cell lines also revealed the overexpression of P-gp and BCRP respectively, and were able to form spheres in non-adherent conditions. Pre-treatment with ATRA and DEAB reduced chemotherapy resistance in both cell lines. ATRA treatment led to downregulation of the ALDH1A1, P-gp and BCRP proteins. DEAB treatment led to downregulation of the P-gp protein and BCRP transcript and protein. Our results indicate that ALDH1A1-positive cancer cells can be responsible for drug resistance development in ovarian cancer. Developing more specific ALDH1A1 inhibitors can increase chemotherapy effectiveness in ovarian cancer.

  15. Quantitative analysis of photoquadrats acquired at Johnston Atoll permanent transect site 2A-P on June 30, 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This spreadsheet summarizes the number of corals photographed along a permanent transect line at Underwater Site 2A-P at Johnston Atoll on 6/30/2000.

  16. AP English language & composition crash course

    CERN Document Server

    Hogue, Dawn

    2012-01-01

    AP English Language & Composition Crash Course - Gets You a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. AP English Language & Composition Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know Crash Course is based on an in-depth analysis of the AP English Language & Composition course description outline and actual Advanced Placement test questions. It covers only the information tested on the exam, so you can make the most of your valua

  17. Microcystin-LR induces anoikis resistance to the hepatocyte uptake transporter OATP1B3-expressing cell lines.

    Science.gov (United States)

    Takano, Hiroyuki; Takumi, Shota; Ikema, Satoshi; Mizoue, Nozomi; Hotta, Yuki; Shiozaki, Kazuhiro; Sugiyama, Yasumasa; Furukawa, Tatsuhiko; Komatsu, Masaharu

    2014-12-04

    Microcystin-LR is a cyclic peptide released by several bloom-forming cyanobacteria. Understanding the mechanism of microcystin-LR toxicity is important, because of the both potencies of its acute cytotoxicity and tumor-promoting activity in hepatocytes of animals and humans. Recently, we have reported that the expression of human hepatocyte uptake transporter OATP1B3 was critical for the selective uptake of microcystin-LR into hepatocytes and for induction of its fatal cytotoxicity. In this study, we demonstrated a novel function of microcystin-LR which induced bipotential changes including anoikis resistance and cytoskeleton reorganization to OATP1B3-transfected HEK293 cells (HEK293-OATP1B3). After exposure to microcystin-LR, HEK293-OATP1B3 cells were divided to the floating cells and remaining adherent cells. After collection and reseeding the floating cells into a fresh flask, cells were confluently proliferated (HEK293-OATP1B3-FL) under the microcystin-LR-free condition. Both the proliferated HEK293-OATP1B3-FL and remaining adherent HEK293-OATP1B3-AD cells changed the character with down- and up-regulation of E-cadherin, respectively. Additionally, these cells acquired resistance to microcystin-LR. These results suggest that microcystin-LR could be associated with not only tumor promotion, but also epithelial-mesenchymal transition-mediated cancer metastasis. Furthermore, microcystin-LR might induce the cytoskeleton reorganization be accompanied epithelial-mesenchymal transition.

  18. Down-regulation of AP-4 inhibits proliferation, induces cell cycle arrest and promotes apoptosis in human gastric cancer cells.

    Directory of Open Access Journals (Sweden)

    Xinghua Liu

    Full Text Available BACKGROUND: AP-4 belongs to the basic helix-loop-helix leucine-zipper subgroup; it controls target gene expression, regulates growth, development and cell apoptosis and has been implicated in tumorigenesis. Our previous studies indicated that AP-4 was frequently overexpressed in gastric cancers and may be associated with the poor prognosis. The purpose of this study is to examine whether silencing of AP-4 can alter biological characteristics of gastric cancer cells. METHODS: Two specific siRNAs targeting AP-4 were designed, synthesized, and transfected into gastric cancer cell lines and human normal mucosa cells. AP-4 expression was measured with real-time quantitative PCR and Western blot. Cell proliferation and chemo-sensitivity were detected by CCK-8 assay. Cell cycle assay and apoptosis assay were performed by flow cytometer, and relative expression of cell cycle regulators were detected by real-time quantitative PCR and Western blot, expression of the factors involved in the apoptosis pathway were examined in mRNA and protein level. RESULTS: The expression of AP-4 was silenced by the siRNAs transfection and the effects of AP-4 knockdown lasted 24 to 96 hrs. The siRNA-mediated silencing of AP-4 suppressed the cellular proliferation, induced apoptosis and sensitized cancer cells to anticancer drugs. In addition, the expression level of p21, p53 and Caspase-9 were increased when AP-4 was knockdown, but the expression of cyclin D1, Bcl-2 and Bcl-x(L was inhibited. It didn't induce cell cycle arrest when AP-4 was knockdown in p53 defect gastric cancer cell line Kato-III. CONCLUSIONS: These results illustrated that gene silencing of AP-4 can efficiently inhibited cell proliferation, triggered apoptosis and sensitized cancer cells to anticancer drugs in vitro, suggesting that AP-4 siRNAs mediated silencing has a potential value in the treatment of human gastric cancer.

  19. Transport of Glial Cell Line-Derived Neurotrophic Factor into Liposomes across the Blood-Brain Barrier: In Vitro and in Vivo Studies

    Directory of Open Access Journals (Sweden)

    Shaoling Wu

    2014-02-01

    Full Text Available Glial cell line-derived neurotrophic factor (GDNF was encapsulated into liposomes in order to protect it from enzyme degradation in vivo and promote its permeability across the blood-brain barrier (BBB. In this study, GDNF conventional liposomes (GDNF-L and GDNF target sterically stabilized liposomes (GDNF-SSL-T were prepared. The average size of liposomes was below 90 nm. A primary model of BBB was established and evaluated by transendothelial electrical resistance (TEER and permeability. This BBB model was employed to study the permeability of GDNF liposomes in vitro. The results indicated that the liposomes could enhance transport of GDNF across the BBB and GDNF-SSL-T had achieved the best transport efficacy. The distribution of GDNF liposomes was studied in vivo. Free GDNF and GDNF-L were eliminated rapidly in the circulation. GDNF-SSL-T has a prolonged circulation time in the blood and favorable brain delivery. The values of the area under the curve (AUC(0–1 h in the brain of GDNF-SSL-T was 8.1 times and 6.8 times more than that of free GDNF and GDNF-L, respectively. These results showed that GDNF-SSL-T realized the aim of targeted delivery of therapeutic proteins to central nervous system.

  20. Transport of glial cell line-derived neurotrophic factor into liposomes across the blood-brain barrier: in vitro and in vivo studies.

    Science.gov (United States)

    Wu, Shaoling; Li, Guoqi; Li, Xiao; Lin, Caina; Yu, Ding; Luan, Shuo; Ma, Chao

    2014-02-27

    Glial cell line-derived neurotrophic factor (GDNF) was encapsulated into liposomes in order to protect it from enzyme degradation in vivo and promote its permeability across the blood-brain barrier (BBB). In this study, GDNF conventional liposomes (GDNF-L) and GDNF target sterically stabilized liposomes (GDNF-SSL-T) were prepared. The average size of liposomes was below 90 nm. A primary model of BBB was established and evaluated by transendothelial electrical resistance (TEER) and permeability. This BBB model was employed to study the permeability of GDNF liposomes in vitro. The results indicated that the liposomes could enhance transport of GDNF across the BBB and GDNF-SSL-T had achieved the best transport efficacy. The distribution of GDNF liposomes was studied in vivo. Free GDNF and GDNF-L were eliminated rapidly in the circulation. GDNF-SSL-T has a prolonged circulation time in the blood and favorable brain delivery. The values of the area under the curve (AUC(0-1 h)) in the brain of GDNF-SSL-T was 8.1 times and 6.8 times more than that of free GDNF and GDNF-L, respectively. These results showed that GDNF-SSL-T realized the aim of targeted delivery of therapeutic proteins to central nervous system.

  1. A high-order unstaggered constrained transport method for the 3D ideal magnetohydrodynamic equations based on the method of lines

    CERN Document Server

    Helzel, Christiane; Taetz, Bertram

    2012-01-01

    Numerical methods for solving the ideal magnetohydrodynamic (MHD) equations in more than one space dimension must confront the challenge of controlling errors in the discrete divergence of the magnetic field. One approach that has been shown successful in stabilizing MHD calculations are constrained transport (CT) schemes. CT schemes can be viewed as predictor-corrector methods for updating the magnetic field, where a magnetic field value is first predicted by a method that does not exactly preserve the divergence-free condition on the magnetic field, followed by a correction step that aims to control these divergence errors. In Helzel et al. (2011) the authors presented an unstaggered constrained transport method for the MHD equations on 3D Cartesian grids. In this work we generalize the method of Helzel et al. (2011) in three important ways: (1) we remove the need for operator splitting by switching to an appropriate method of lines discretization and coupling this with a non-conservative finite volume meth...

  2. THE EVALUATION OF PEPTIDE/HISTIDINE TRANSPORTER 1 (PHT1) FUNCTION: UPTAKE KINETICS UTILIZING A COS-7 STABLY TRANSFECTED CELL LINE.

    Science.gov (United States)

    Lindley, David J; Carl, Stephen M; Mowery, Stephanie A; Knipp, Gregory T

    2011-10-01

    There have been relatively few studies focused on the proton-dependent oligopeptide transporter (POT) superfamily member, Peptide/Histidine Transporter 1 (PHT1), with respect to its contribution to the ADME of peptides and peptide-based drugs. These studies were conducted to determine hPHT1-mediated, H(+)-dependent uptake kinetics of histidine, carnosine, Gly-Sar and valacyclovir in stably transfected hPHT1-COS-7 cells comparative to kinetics determined in an empty vector (Mock) stably transfected cell line. The results suggest that Gly-Sar appears to be a substrate for PHT1 based on efflux from the stably transfected hPHT1 COS-7 cells. Histidine and Gly-Sar concentration- and time-dependent studies suggest mixed-uptake kinetics. These studies suggest that stably transfected hPHT1-COS-7 cells exhibit different uptake kinetics than those observed in our previous studies and illustrate the requirement for experiments to delineate the physiological role of hPHT1.

  3. Pentoxifylline Treatment in Acute Pancreatitis (AP)

    Science.gov (United States)

    2016-09-14

    Acute Pancreatitis (AP); Gallstone Pancreatitis; Alcoholic Pancreatitis; Post-ERCP/Post-procedural Pancreatitis; Trauma Acute Pancreatitis; Hypertriglyceridemia Acute Pancreatitis; Idiopathic (Unknown) Acute Pancreatitis; Medication Induced Acute Pancreatitis; Cancer Acute Pancreatitis; Miscellaneous (i.e. Acute on Chronic Pancreatitis)

  4. High energy beam lines

    Science.gov (United States)

    Marchetto, M.; Laxdal, R. E.

    2014-01-01

    The ISAC post accelerator comprises an RFQ, DTL and SC-linac. The high energy beam lines connect the linear accelerators as well as deliver the accelerated beams to two different experimental areas. The medium energy beam transport (MEBT) line connects the RFQ to the DTL. The high energy beam transport (HEBT) line connects the DTL to the ISAC-I experimental stations (DRAGON, TUDA-I, GPS). The DTL to superconducting beam (DSB) transport line connects the ISAC-I and ISAC-II linacs. The superconducting energy beam transport (SEBT) line connects the SC linac to the ISAC-II experimental station (TUDA-II, HERACLES, TIGRESS, EMMA and GPS). All these lines have the function of transporting and matching the beams to the downstream sections by manipulating the transverse and longitudinal phase space. They also contain diagnostic devices to measure the beam properties.

  5. Peculiar Traits of Coarse AP (Briefing Charts)

    Science.gov (United States)

    2014-12-01

    Self Combustion 3 Thom L. Boggs Parallel ridges and valley surfaces seen at 1000 to 1500 psi Isolated AP vertical needle pockets at 1500 and 1800 psi...Negative exponents, bouncing flamlets between 2000 to 4000 psi Extremely irregular AP surface regression Hightower and Price 16 Air Force Research...supported by vertical gas jetlets from active centers? Did Boggs burn a few samples at 1000 to 2000 psi with orthorhombic main axis normal to

  6. Tank 241-AP-106, Grab samples, 6AP-98-1, 6AP-98-2 and 6AP-98-3 Analytical results for the final report

    Energy Technology Data Exchange (ETDEWEB)

    FULLER, R.K.

    1999-02-23

    This document is the final report for tank 241-AP-106 grab samples. Three grab samples 6AP-98-1, 6AP-98-2 and 6AP-98-3 were taken from riser 1 of tank 241-AP-106 on May 28, 1998 and received by the 222-S Laboratory on May 28, 1998. Analyses were performed in accordance with the ''Compatability Grab Sampling and Analysis Plan'' (TSAP) (Sasaki, 1998) and the ''Data Quality Objectives for Tank Farms Waste Compatability Program (DQO). The analytical results are presented in the data summary report. No notification limits were exceeded. The request for sample analysis received for AP-106 indicated that the samples were polychlorinated biphenyl (PCB) suspects. The results of this analysis indicated that no PCBs were present at the Toxic Substance Control Act (TSCA) regulated limit of 50 ppm. The results and raw data for the PCB analysis are included in this document.

  7. Importância da ventilação pulmonar no transporte de 0(2 e equilíbrio ácido-base após desclampeamento intermitente de aorta na revascularização cirúrgica do miocárdio: desclamping in coronary artery bypass graft Importance of lung ventilation on oxygen transport and acid-base balance after intermittent aortic cross

    Directory of Open Access Journals (Sweden)

    Marilde de Albuquerque Piccioni

    1991-12-01

    Full Text Available Na revascularização cirúrgica do miocárdio, empregando-se a técnica de pinçamento intermitente de aorta, após o desclampeamento e a recuperação dos batimentos cardíacos, surgiu a polêmica da necessidade da ventilação pulmonar na prevenção de hipoxemia. O objetivo deste trabalho foi analisar a importância da ventilação pulmonar no transporte de oxigênio e equilíbrio ácido-base do sangue que irá perfundir o miocárdio após desclampeamento de aorta e recuperação dos batimentos cardíacos. Foram estudados dez pacientes submetidos a revascularização cirúrgica do miocárdio, empregando-se a técnica de pinçamento intermitente de aorta com hipotermia moderada (± 32ºC. Em cinco pacientes (Grupo I, após o 1º desclampeamento de aorta, a ventilação pulmonar foi realizada simultaneamente à recuperação dos batimentos cardíacos. Nos outros cinco pacientes (Grupo II não se realizou a ventilação pulmonar. Foram analisados (Saturação de O2, PO2, PCO2 e pH do sangue do átrio direito (AD, tronco pulmonar (AP, átrio esquerdo (AE, aorta (Ao, artéria radial (art. radial e circuito da circulação extracorpórea (CEC (arterial [art. CEC] e venoso [ven. CEC], comparando-se os dois grupos. Não houve diferença estatisticamente nos valores de saturação de 02 em ambos os grupos. No Grupo I, os valores do PO2 aumentaram significativamente. Houve aumento significante da PO2 no Grupo II, contribuindo para redução significante de pH neste grupo. Os autores concluem que, embora não tenha ocorrido hipoxemia, a acidose respiratória observada no Grupo II permite recomendar a ventilação pulmonar ao utilizar-se do pinçamento intermitente de aorta como forma de proteção miócardica, pois sabese dos efeitos deletérios do aumento da concentração de íons hidrogênio ([H+] na função contrátil do miocárdio.In coronary artery bypass graft employing the surgical technique of intermittent aortic cross-clamping appeared the

  8. The hotline in the grid. Higher temperatures with overhead lines can help to transport additional electricity; Der heisse Draht im Netz. Hoehere Temperaturen bei Freileitungen koennen helfen, Stromueberschuesse zu transportieren

    Energy Technology Data Exchange (ETDEWEB)

    Michels, Andreas; Friedrich, Uwe

    2016-11-01

    The electricity grid that has been used for decades reaches its limits when transporting increasing amounts of electricity over long distances. There are already limiting bottlenecks. One way to integrate more wind and solar power into the grid is provided by overhead lines with a higher maximum temperature. These can transport more electricity, enabling grid congestion to be avoided. One problem, though, is that metals expand more at high temperatures. As a result the line lengthens and sags more. However, special high-temperature conductors can provide a remedy here.

  9. Knockdown of CDK2AP1 in primary human fibroblasts induces p53 dependent senescence.

    Directory of Open Access Journals (Sweden)

    Khaled N Alsayegh

    Full Text Available Cyclin Dependent Kinase-2 Associated Protein-1 (CDK2AP1 is known to be a tumor suppressor that plays a role in cell cycle regulation by sequestering monomeric CDK2, and targeting it for proteolysis. A reduction of CDK2AP1 expression is considered to be a negative prognostic indicator in patients with oral squamous cell carcinoma and also associated with increased invasion in human gastric cancer tissue. CDK2AP1 overexpression was shown to inhibit growth, reduce invasion and increase apoptosis in prostate cancer cell lines. In this study, we investigated the effect of CDK2AP1 downregulation in primary human dermal fibroblasts. Using a short-hairpin RNA to reduce its expression, we found that knockdown of CDK2AP1 in primary human fibroblasts resulted in reduced proliferation and in the induction of senescence associated beta-galactosidase activity. CDK2AP1 knockdown also resulted in a significant reduction in the percentage of cells in the S phase and an accumulation of cells in the G1 phase of the cell cycle. Immunocytochemical analysis also revealed that the CDK2AP1 knockdown significantly increased the percentage of cells that exhibited γ-H2AX foci, which could indicate presence of DNA damage. CDK2AP1 knockdown also resulted in increased mRNA levels of p53, p21, BAX and PUMA and p53 protein levels. In primary human fibroblasts in which p53 and CDK2AP1 were simultaneously downregulated, there was: (a no increase in senescence associated beta-galactosidase activity, (b decrease in the number of cells in the G1-phase and increase in number of cells in the S-phase of the cell cycle, and (c decrease in the mRNA levels of p21, BAX and PUMA when compared with CDK2AP1 knockdown only fibroblasts. Taken together, this suggests that the observed phenotype is p53 dependent. We also observed a prominent increase in the levels of ARF protein in the CDK2AP1 knockdown cells, which suggests a possible role of ARF in p53 stabilization following CDK2AP1

  10. Kepler observations of rapidly oscillating Ap, δ Scuti and γ Doradus pulsations in Ap stars

    DEFF Research Database (Denmark)

    Balona, Luis A.; Cunha, Margarida S.; Kurtz, Donald W.

    2011-01-01

    Observations of the A5p star KIC 8677585 obtained during the Kepler 10-d commissioning run with 1-min time resolution show that it is a rapidly oscillating Ap (roAp) star with several frequencies with periods near 10 min. In addition, a low frequency at 3.142 d−1 is also clearly present....... Multiperiodic γ Doradus (γ Dor) and δ Scuti (δ Sct) pulsations, never before seen in any Ap star, are present in Kepler observations of at least three other Ap stars. Since γ Dor pulsations are seen in Ap stars, it is likely that the low frequency in KIC 8677585 is also a γ Dor pulsation. The simultaneous...... presence of both γ Dor and roAp pulsations and the unexpected detection of δ Sct and γ Dor pulsations in Ap stars present new opportunities and challenges for the interpretation of these stars. Since it is easy to confuse Am and Ap stars at classification dispersions, the nature of these Ap stars...

  11. Over-expression of the PaAP1 gene from sweet cherry (Prunus avium L.) causes early flowering in Arabidopsis thaliana.

    Science.gov (United States)

    Wang, Jing; Zhang, Xiaoming; Yan, Guohua; Zhou, Yu; Zhang, Kaichun

    2013-02-15

    A homologue of SQUAMOSA/APETALA1, designated PaAP1, was isolated from Prunus avium by reverse transcription-PCR (RT-PCR). The full length of PaAP1 cDNA is 753 bp, and it codes for a polypeptide of 250 amino acid residues. Sequence comparison revealed that PaAP1 belongs to the MADS-box gene family. Phylogenetic analysis indicated that PaAP1 shared the highest identity with SQUA/AP1 homologues from Prunus serrulata. Real-time fluorescence quantitative PCR analysis showed that PaAP1 was expressed at high levels in petal, sepal, style, and flower buds, which was slightly different from the expression pattern of AP1 of Arabidopsis thaliana. To characterize the functions of PaAP1, we assessed Arabidopsis transformed with 35S::PaAP1. A total of 8 transgenic T(1) lines with an early flowering phenotype were obtained, and a 3:1 segregation ratio of flowering time was observed in the T(2) generation of 4 lines. This study provides the first functional analysis of an SQUA/AP1 homolog from P. avium and suggests that PaAP1 is potentially useful for shortening the juvenile period in sweet cherry.

  12. The AP-3 adaptor complex is required for vacuolar function in Arabidopsis

    Institute of Scientific and Technical Information of China (English)

    Maria Zwiewka; Elena Feraru; Barbara M(o)ller; Inhwan Hwang; Mugurel I Feraru; Jürgen Kleine-Vehn; Dolf Weijers; Ji(n) Friml

    2011-01-01

    Subcellular trafficking is required for a multitude of functions in eukaryotic cells.It involves regulation of cargo sorting,vesicle formation,trafficking and fusion processes at multiple levels.Adaptor protein (AP) complexes are key regulators of cargo sorting into vesicles in yeast and mammals but their existence and function in plants have not been demonstrated.Here we report the identification of the protein-affected trafficking 4 (pat4) mutant defective in the putative δ subunit of the AP-3 complex.pat4 and pat2,a mutant isolated from the same GFP imaging-based forward genetic screen that lacks a functional putative AP-3 β,as well as dominant negative AP-3 μ transgenic lines display undistinguishable phenotypes characterized by largely normal morphology and development,but strong intracellular accumulation of membrane proteins in aberrant vacuolar structures.All mutants are defective in morphology and function of lytic and protein storage vacuoles (PSVs) but show normal sorting of reserve proteins to PSVs.Immunoprecipitation experiments and genetic studies revealed tight functional and physical associations of putative AP-3 β and AP-3 δ subunits.Furthermore,both proteins are closely linked with putative AP-3 μ and σ subunits and several components of the clathrin and dynamin machineries.Taken together,these results demonstrate that AP complexes,similar to those in other eukaryotes,exist in plants,and that AP-3 plays a specific role in the regulation of biogenesis and function of vacuoles in plant cells.

  13. Loss of AP-5 results in accumulation of aberrant endolysosomes: defining a new type of lysosomal storage disease.

    Science.gov (United States)

    Hirst, Jennifer; Edgar, James R; Esteves, Typhaine; Darios, Frédéric; Madeo, Marianna; Chang, Jaerak; Roda, Ricardo H; Dürr, Alexandra; Anheim, Mathieu; Gellera, Cinzia; Li, Jun; Züchner, Stephan; Mariotti, Caterina; Stevanin, Giovanni; Blackstone, Craig; Kruer, Michael C; Robinson, Margaret S

    2015-09-01

    Adaptor proteins (AP 1-5) are heterotetrameric complexes that facilitate specialized cargo sorting in vesicular-mediated trafficking. Mutations in AP5Z1, encoding a subunit of the AP-5 complex, have been reported to cause hereditary spastic paraplegia (HSP), although their impact at the cellular level has not been assessed. Here we characterize three independent fibroblast lines derived from skin biopsies of patients harbouring nonsense mutations in AP5Z1 and presenting with spastic paraplegia accompanied by neuropathy, parkinsonism and/or cognitive impairment. In all three patient-derived lines, we show that there is complete loss of AP-5 ζ protein and a reduction in the associated AP-5 µ5 protein. Using ultrastructural analysis, we show that these patient-derived lines consistently exhibit abundant multilamellar structures that are positive for markers of endolysosomes and are filled with aberrant storage material organized as exaggerated multilamellar whorls, striated belts and 'fingerprint bodies'. This phenotype can be replicated in a HeLa cell culture model by siRNA knockdown of AP-5 ζ. The cellular phenotype bears striking resemblance to features described in a number of lysosomal storage diseases (LSDs). Collectively, these findings reveal an emerging picture of the role of AP-5 in endosomal and lysosomal homeostasis, illuminates a potential pathomechanism that is relevant to the role of AP-5 in neurons and expands the understanding of recessive HSPs. Moreover, the resulting accumulation of storage material in endolysosomes leads us to propose that AP-5 deficiency represents a new type of LSDs.

  14. Characterization of rPEPT2-mediated Gly-Sar transport parameters in the rat kidney proximal tubule cell line SKPT-0193 cl.2 cultured in basic growth media

    DEFF Research Database (Denmark)

    Bravo, Silvina A; Nielsen, Carsten Uhd; Frokjaer, Sven;

    2005-01-01

    added. The aim of the study was thus to characterize Gly-Sar transport parameters in SKPT cells cultured in basic growth media (conventional media without added agonists). Morphology was studied using confocal laser scanning microscopy (CLSM) and immunohistochemistry. Monolayer integrity was evaluated......The rat proximal kidney tubule cell line SKPT-0193 cl.2 (SKPT) expresses the di-/tripeptide transporter PEPT2 (rPEPT2) and has been used to study PEPT2-mediated transport. Traditionally, SKPT cells have been cultured in growth media supplemented with epidermal growth factor (EGF), apotransferrin...

  15. Multiplicity of rapidly oscillating Ap stars

    CERN Document Server

    Schoeller, M; Hubrig, S; Kurtz, D W

    2012-01-01

    Rapidly oscillating Ap (roAp) stars have rarely been found in binary or higher order multiple systems. This might have implications for their origin. We intend to study the multiplicity of this type of chemically peculiar stars, looking for visual companions in the range of angular separation between 0.05" and 8". We carried out a survey of 28 roAp stars using diffraction-limited near-infrared imaging with NAOS-CONICA at the VLT. Additionally, we observed three non-oscillating magnetic Ap stars. We detected a total of six companion candidates with low chance projection probabilities. Four of these are new detections, the other two are confirmations. An additional 39 companion candidates are very likely chance projections. We also found one binary system among the non-oscillating magnetic Ap stars. The detected companion candidates have apparent K magnitudes between 6.8 and 19.5 and angular separations ranging from 0.23" to 8.9", corresponding to linear projected separations of 30-2400AU. While our study confi...

  16. Center for Geometrisk Metrologi CGM ApS, Årsberetning 2001 til DANAK

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2001. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, Erhvervsfremme Styrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler (Teknisk Forskrift Nr. TF4 af 2000...

  17. AP1000, a nuclear central of advanced design; AP1000, una central nuclear de diseno avanzado

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez M, N.; Viais J, J. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)]. e-mail: nhm@nuclear.inin.mx

    2005-07-01

    The AP1000 is a design of a nuclear reactor of pressurized water (PWR) of 1000 M We with characteristic of safety in a passive way; besides presenting simplifications in the systems of the plant, the construction, the maintenance and the safety, the AP1000 is a design that uses technology endorsed by those but of 30 years of operational experience of the PWR reactors. The program AP1000 of Westinghouse is focused to the implementation of the plant to provide improvements in the economy of the same one and it is a design that is derived directly of the AP600 designs. On September 13, 2004 the US-NRC (for their initials in United States- Nuclear Regulatory Commission) approved the final design of the AP1000, now Westinghouse and the US-NRC are working on the whole in a complete program for the certification. (Author)

  18. Regulation of Aggression by Obesity-Linked Genes TfAP-2 and Twz Through Octopamine Signaling in Drosophila

    Science.gov (United States)

    Williams, Michael J.; Goergen, Philip; Rajendran, Jayasimman; Klockars, Anica; Kasagiannis, Anna; Fredriksson, Robert; Schiöth, Helgi B.

    2014-01-01

    In Drosophila, the monoamine octopamine, through mechanisms that are not completely understood, regulates both aggression and mating behavior. Interestingly, our study demonstrates that the Drosophila obesity-linked homologs Transcription factor AP-2 (TfAP-2; TFAP2B in humans) and Tiwaz (Twz; KCTD15 in humans) interact to modify male behavior by controlling the expression of Tyramine β-hydroxylase and Vesicular monanime transporter, genes necessary for octopamine production and secretion. Furthermore, we reveal that octopamine in turn regulates aggression through the Drosophila cholecystokinin satiation hormone homolog Drosulfakinin (Dsk). Finally, we establish that TfAP-2 is expressed in octopaminergic neurons known to control aggressive behavior and that TfAP-2 requires functional Twz for its activity. We conclude that genetically manipulating the obesity-linked homologs TfAP-2 and Twz is sufficient to affect octopamine signaling, which in turn modulates Drosophila male behavior through the regulation of the satiation hormone Dsk. PMID:24142897

  19. Formalistic approaches to A&P

    Institute of Scientific and Technical Information of China (English)

    赵倩

    2014-01-01

    A&P is a famous short story. Its simple words, the unique point of view and skillful writing technique are expressed successfully. Besides, the first-person narrator is also a good point. From Sammy’s eyes, readers can learn the character’s characteristics. Another point is the usage of symbol, the name of the store, A&P, it shows that people’s life is rich, but their spirit is barren. This article tries to analyze it by formalistic approaches.

  20. An analysis of AP600 design features

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jong Kyoon; Jang, Moon Heui; Hwang, Yung Dong [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)] [and others

    1994-01-01

    In the aspect of engineering, passive safety system concept has improved the safety degree of nuclear power plant. Therefore, the objective of this study is to check on the possibility of the capacity upgrade of nuclear power plant in the case of adopting the passive safety system concept of AP 600. The characteristics of AP 600 are the advanced functions in ECCS, heat removal of containment building and residual heat removal under the passive safety system concept. The result of this study will become the basic data of capacity upgrade of nuclear power plant and will be widely used in second year project. (Author).

  1. Chlamydia trachomatis CT771 (nudH) is an asymmetric Ap4A hydrolase

    Science.gov (United States)

    Barta, Michael L.; Lovell, Scott; Sinclair, Amy N.; Battaile, Kevin P.; Hefty, P. Scott

    2014-01-01

    Asymmetric diadenosine 5′,5′″-P1,P4-tetraphosphate (Ap4A) hydrolases are members of the Nudix superfamily that asymmetrically cleave the metabolite Ap4A into ATP and AMP while facilitating homeostasis. The obligate intracellular mammalian pathogen Chlamydia trachomatis possesses a single Nudix family protein, CT771. As pathogens that rely on a host for replication and dissemination typically have one or zero Nudix family proteins, this suggests that CT771 could be critical for chlamydial biology and pathogenesis. We identified orthologs to CT771 within environmental Chlamydiales that share active site residues suggesting a common function. Crystal structures of both apo- and ligand-bound CT771 were determined to 2.6 Å and 1.9 Å resolution, respectively. The structure of CT771 shows a αβα-sandwich motif with many conserved elements lining the putative Nudix active site. Numerous aspects of the ligand-bound CT771 structure mirror those observed in the ligand-bound structure of the Ap4A hydrolase from Caenorhabditis elegans. These structures represent only the second Ap4A hydrolase enzyme member determined from eubacteria and suggest that mammalian and bacterial Ap4A hydrolases might be more similar than previously thought. The aforementioned structural similarities, in tandem with molecular docking, guided the enzymatic characterization of CT771. Together, these studies provide the molecular details for substrate binding and specificity, supporting the analysis that CT771 is an Ap4A hydrolase (nudH). PMID:24354275

  2. Comparison of a CCD and an APS for soft X-ray diffraction

    Science.gov (United States)

    Stewart, Graeme; Bates, R.; Blue, A.; Clark, A.; Dhesi, S. S.; Maneuski, D.; Marchal, J.; Steadman, P.; Tartoni, N.; Turchetta, R.

    2011-12-01

    We compare a new CMOS Active Pixel Sensor (APS) to a Princeton Instruments PIXIS-XO: 2048B Charge Coupled Device (CCD) with soft X-rays tested in a synchrotron beam line at the Diamond Light Source (DLS). Despite CCDs being established in the field of scientific imaging, APS are an innovative technology that offers advantages over CCDs. These include faster readout, higher operational temperature, in-pixel electronics for advanced image processing and reduced manufacturing cost. The APS employed was the Vanilla sensor designed by the MI3 collaboration and funded by an RCUK Basic technology grant. This sensor has 520 x 520 square pixels, of size 25 μm on each side. The sensor can operate at a full frame readout of up to 20 Hz. The sensor had been back-thinned, to the epitaxial layer. This was the first time that a back-thinned APS had been demonstrated at a beam line at DLS. In the synchrotron experiment soft X-rays with an energy of approximately 708 eV were used to produce a diffraction pattern from a permalloy sample. The pattern was imaged at a range of integration times with both sensors. The CCD had to be operated at a temperature of -55°C whereas the Vanilla was operated over a temperature range from 20°C to -10°C. We show that the APS detector can operate with frame rates up to two hundred times faster than the CCD, without excessive degradation of image quality. The signal to noise of the APS is shown to be the same as that of the CCD at identical integration times and the response is shown to be linear, with no charge blooming effects. The experiment has allowed a direct comparison of back thinned APS and CCDs in a real soft x-ray synchrotron experiment.

  3. 2000 Johnston Site 3A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 3A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on July 3, 2000. With a start point (meter 0) at...

  4. 2000 Johnston Site 1A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 1A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on June 29, 2000. With a start point (meter 0) at...

  5. 2000 Johnston Site 2A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 2A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on June 30, 2000. With a start point (meter 0) at...

  6. 2006 Johnston Site 1A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 1A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on June 29, 2000. With a start point (meter 0) at...

  7. Structuring the AP Art History Course

    Science.gov (United States)

    Herscher, Walter R.

    2013-01-01

    While AP (Advanced Placement) Art History may be taught within the art department in many schools, social studies teachers are equally capable of teaching the course well. They have the historical background to discuss the reasons for changes in art styles. A teacher's preparation is similar to teaching a course stressing political history,…

  8. Barron's AP English literature and composition

    CERN Document Server

    Ehrenhaft EdD, George

    2016-01-01

    Includes five full-length practice AP exams with all questions answered and explained. Also features additional reviews on poetry, fiction, and drama, definitions of 175 literary and rhetorical terms, and more. Can be purchased alone or with an optional CD-ROM with two additional practice tests.

  9. Two Successful Approaches to Teaching AP Government

    Science.gov (United States)

    Ladd, Brian; Stepp, Heidi

    2013-01-01

    Amador Valley High School, in Pleasanton, California, uses two unique approaches to teaching Advanced Placement Government and Politics. AP Government consists of six units: Constitutional Underpinnings; Political Behavior and Political Beliefs; Mass Media, Interest Groups, and Political Parties; Institutions of Government; Civil Liberties and…

  10. Project W-519 CDR supplement: Raw water and electrical services for privatization contractor, AP tank farm operations

    Energy Technology Data Exchange (ETDEWEB)

    Parazin, R.J.

    1998-07-31

    This supplement to the Project W-519 Conceptual Design will identify a means to provide RW and Electrical services to serve the needs of the TWRS Privatization Contractor (PC) at AP Tank Farm as directed by DOE-RL. The RW will serve the fire suppression and untreated process water requirements for the PC. The purpose of this CDR supplement is to identify Raw Water (RW) and Electrical service line routes to the TWRS Privatization Contractor (PC) feed delivery tanks, AP-106 and/or AP-108, and establish associated cost impacts to the Project W-519 baseline.

  11. Overexpression of the Lotus corniculatus Soloist Gene LcAP2/ERF107 Enhances Tolerance to Salt Stress.

    Science.gov (United States)

    Sun, Zhan-Min; Zhou, Mei-Liang; Dan-Wang; Tang, Yi-Xiong; Lin, Min; Wu, Yan-Min

    2016-01-01

    The AP2/ERF play a key role in multiple stress responses in plants. we here report a novel salt stress-related gene, LcAP2/ERF107 that encodes an AP2/ERF protein in Lotus corniculatus cultivar Leo. LcAP2/ERF107 was classified into the soloist subfamiliy based on phylogenetic relationship. The transcription of LcAP2/ERF107 were strongly induced by salt and other phytohormones (ABA, ACC, MeJA). A subcellular localization experiment indicated that LcAP2/ERF107 is a nuclear protein that activates transcription. LcAP2/ERF107 overexpression in Arabidopsis resulted in pleiotropic phenotypes, including higher seed germination rate and transgenic plants with enhanced tolerance to salt stress. Further, under salt tolerance the transgenic lines elevated the relative moisture content; however, the relative electrolyte leakage was lower than in control plants. The expression levels of indicative genes RD22, RD29A, LEA4-5, P5CS1 and P5CS2 were found to be increased in the transgenic plants compared with the WT plants. These results indicated that LcAP2/ERF107 play an important role in the responses of plant to salt stress.

  12. Luteolin, a flavonoid, inhibits AP-1 activation by basophils.

    Science.gov (United States)

    Hirano, Toru; Higa, Shinji; Arimitsu, Junsuke; Naka, Tetsuji; Ogata, Atsushi; Shima, Yoshihito; Fujimoto, Minoru; Yamadori, Tomoki; Ohkawara, Tomoharu; Kuwabara, Yusuke; Kawai, Mari; Matsuda, Hisashi; Yoshikawa, Masayuki; Maezaki, Naoyoshi; Tanaka, Tetsuaki; Kawase, Ichiro; Tanaka, Toshio

    2006-02-03

    Flavonoids including luteolin, apigenin, and fisetin are inhibitors of IL-4 synthesis and CD40 ligand expression by basophils. This study was done to search for compounds with greater inhibitory activity of IL-4 expression and to clarify the molecular mechanisms through which flavonoids inhibit their expression. Of the 37 flavonoids and related compounds examined, ayanin, luteolin, and apigenin were the strongest inhibitors of IL-4 production by purified basophils in response to anti-IgE antibody plus IL-3. Luteolin did not suppress Syk or Lyn phosphorylation in basophils, nor did suppress p54/46 SAPK/JNK, p38 MAPK, and p44/42 MAPK activation by a basophilic cell line, KU812 cells, stimulated with A23187 and PMA. However, luteolin did inhibit phosphorylation of c-Jun and DNA binding activity of AP-1 in nuclear lysates from stimulated KU812 cells. These results provide a fundamental structure of flavonoids for IL-4 inhibition and demonstrate a novel action of flavonoids that suppresses the activation of AP-1.

  13. Abnormal lithium abundance in several Ap-Bp stars

    CERN Document Server

    Shavrina, A; Drake, N A; Kudryavtsev, D O; Gopka, V F; Yushchenko, V A; Yushchenko, A V

    2013-01-01

    Using observations of rapidly rotating roAp star HD 12098, obtained with 6m telescope of SAO RAS in 2005-2008 in nine rotational phases (0.001-0.817), and code V. Tsymbal ROTATE we identified 3 lithium spots on the surface of star, one of them coincided with the position of one magnetic pole and two others are located near the other pole, symmetrically with respect to it. Perhaps magnetic field of the star has a more complex structure than the dipole (Shavrina 2008). Lithium abundance in the spots is greater than cosmic one. It can be explained by diffusion of lithium in the presence of a magnetic field or by production of lithium in the reactions of "spallation" on the surface of star near the magnetic poles (see, for example, Goriely 2007). Enhanced lithium abundances was also determined for other two slowly rotating Ap stars HR 465 and HD 965. The analysis is performed using a synthetic spectrum method taking into account magnetic splitting of lines by the SYNTHM code of Khan (2004).

  14. Analysis on the Track Quality Situation of Jiaoji Passenger Transport Line%胶济客运专线轨道质量状态分析

    Institute of Scientific and Technical Information of China (English)

    张紫菱

    2015-01-01

    目前有砟轨道在我国高速铁路中占有重要地位,研究有砟轨道质量状态的发展对高速铁路的养护维修工作具有重要意义。从轨道不平顺指数方面对胶济客运专线的轨道检查数据进行了分析,发现单项指数中高低、轨向、水平和三角坑的影响相对较大,维修周期内轨道TQI指数在上下波动的同时逐渐增大并呈线性增长趋势,TQI指数增加到7左右时安排大机维修作业较为合理,大机维修作业后轨道TQI指数可以降低到4.5左右。%At present, ballast track occupies an important place in the high-speed railway of China. It has important significance to study the quality development of ballast track for the maintenance and repair work of high-speed railway. The track inspection data of Jiaoji passenger transport line is analyzed from the track irregularity index. It finds that the effect of the single index such as discretion, track alignment, horizontal and twist warp is bigger. TQI index of track fluctuates, at the same time it gradually increases and has a linear growth trend in the maintenance cycle. When TQI index increases to about 7, the arrangement of big machine maintenance work is more reasonable. After the big machine maintenance work, TQI index can reduce to about 4.5.

  15. Transcriptomic analysis by RNA-seq reveals AP-1 pathway as key regulator that green tea may rely on to inhibit lung tumorigenesis.

    Science.gov (United States)

    Pan, Jing; Zhang, Qi; Xiong, Donghai; Vedell, Peter; Yan, Ying; Jiang, Hui; Cui, Peng; Ding, Feng; Tichelaar, Jay W; Wang, Yian; Lubet, Ronald A; You, Ming

    2014-01-01

    Green tea is a promising chemopreventive agent for lung cancer. Multiple signaling events have been reported, however, the relative importance of these mechanisms in mediating the chemopreventive function of green tea is unclear. In the present study, to examine the involvement of AP-1 in green tea polyphenols induced tumor inhibition, human NSCLC cell line H1299 and mouse SPON 10 cells were identified as AP-1 dependent, as these two lines exhibit high constitutive AP-1 activity, and when TAM67 expression was induced with doxycycline, cell growth was inhibited and correlated with suppressed AP-1 activity. RNA-seq was used to determine the global transcriptional effects of AP-1 inhibition and also uncover the possible involvement of AP-1 in tea polyphenols induced chemoprevention. TAM67 mediated changes in gene expression were identified, and within down-regulated genes, AP-1 was identified as a key transcription regulator. RNA-seq analysis revealed that Polyphenon E-treated cells shared 293 commonly down-regulated genes within TAM67 expressing H1299 cells, and by analysis of limited Chip-seq data, over 10% of the down-regulated genes contain a direct AP-1 binding site, indicating that Polyphenon E elicits chemopreventive activity by regulating AP-1 target genes. Conditional TAM67 expressing transgenic mice and NSCLC cell lines were used to further confirm that the chemopreventive activity of green tea is AP-1 dependent. Polyphenon E lost its chempreventive function both in vitro and in vivo when AP-1 was inhibited, indicating that AP-1 inhibition is a major pathway through which green tea exhibits chemopreventive effects.

  16. AE8/AP8 Implementations in AE9/AP9, IRBEM, and SPENVIS

    Science.gov (United States)

    2014-02-18

    period applies to orbit generation only; AE8/AP8 utilizes geomagnetic field models from other epochs as specified in the table below.) SHIELDOSE2 model...Cain, J. C., S. J. Hendricks, R. A. Langel, and W. V. Hudson (1967), A proposed model for the international geomagnetic reference field, 1965, J...trapped particle fluxes with the NASA models AP-8 and AE-8, Radiat. Meas., 26, pp. 947-952. International Association of Geomagnetism and Aeronomy

  17. Comparative analysis between radiographic views for knee osteoarthrosis (bipedal AP versus monopedal AP

    Directory of Open Access Journals (Sweden)

    Rodrigo Pires e Albuquerque

    2013-08-01

    Full Text Available OBJECTIVE: A comparative analysis by applying the criteria of the original classification Ahlbäck in the anteroposterior (AP bipedal knee in extension and anteroposterior (AP monopodal knee in symptomatic knee arthrosis. With this analysis we intend to observe the agreement, any advantage or difference between the incidence and degree of joint involvement between the orthopedic surgeons and radiologists with the referring physician. METHODS: From January 2012 to March 2012, was a prospective study of 60 symptomatic arthrosis knees (60 patients, clinically selected group of outpatient knee and radiographic proposals submitted to the search. Of the 60 patients, 39 were female and 21 male, mean age 64 years (ranging from 50 to 84 years. Of the 60 knees studied, 37 corresponded to the right side and 23 on the left side. Statistical analysis was performed by Kappa statistics, which evaluates the interobserver agreement for qualitative data. RESULTS: According to the scale of Ahlbäck, there was a significant agreement (p < 0.0001 intra-observer in the classification of knee osteoarthritis among the five evaluators. There was a significant agreement (p < 0.0001 with inter-observer referring physician in the incidence of AP monopodal and AP bipedal for the four raters. CONCLUSION: The study found no difference between the incidence in the AP monopodal versus AP bipedal in osteoarthritis of the knee.

  18. Final report for tank 241-AP-108, grab samples 8AP-96-1, 8AP-96-2 and 8AP-96-FB

    Energy Technology Data Exchange (ETDEWEB)

    Esch, R.A.

    1996-04-19

    This document is the final report deliverable for the tank 241-AP-108 grab samples. The samples were subsampled and analyzed in accordance with the TSAP. Included in this report are the results for the Waste Compatibility analyses, with the exception of DSC and thermogravimetric analysis (TGA) results which were presented in the 45 Day report (Part 2 of this document). The raw data for all analyses, with the exception of DSC and TGA, are also included in this report.

  19. An Updated AP2 Beamline TURTLE Model

    Energy Technology Data Exchange (ETDEWEB)

    Gormley, M.; O' Day, S.

    1991-08-23

    This note describes a TURTLE model of the AP2 beamline. This model was created by D. Johnson and improved by J. Hangst. The authors of this note have made additional improvements which reflect recent element and magnet setting changes. The magnet characteristics measurements and survey data compiled to update the model will be presented. A printout of the actual TURTLE deck may be found in appendix A.

  20. Using DCAT-AP for research data

    OpenAIRE

    PEREGO ANDREA; FRIIS-CHRISTENSEN Anders; VACCARI LORENZINO; TSINARAKI CHRYSI

    2016-01-01

    This paper outlines a set cross-domain requirements for the documentation of scientific data, identified during the development of the corporate data catalogue of the European Commission's Joint Research Centre (JRC). In particular, we illustrate how we have extended the DCAT application profile for European data portals (DCAT-AP) to accomodate requirements for scientific datasets, and we discuss a number of issues still to be addressed.

  1. Cultural Diversity in AP Art History

    Science.gov (United States)

    Bolte, Frances R.

    2006-01-01

    Teaching AP Art History is like running on a treadmill that is moving faster than a teacher can run. Many teachers are out of breath before the end of the term and wonder how in the world they can cover every chapter. Because time is short and art from pre-history through to the present, including the non-European traditions, must be covered, this…

  2. Task Force on Catastrophic Antiphospholipid Syndrome (APS) and Non-criteria APS Manifestations (II): thrombocytopenia and skin manifestations.

    Science.gov (United States)

    Cervera, R; Tektonidou, M G; Espinosa, G; Cabral, A R; González, E B; Erkan, D; Vadya, S; Adrogué, H E; Solomon, M; Zandman-Goddard, G; Shoenfeld, Y

    2011-02-01

    The objectives of the 'Task Force on Catastrophic Antiphospholipid Syndrome (APS) and Non-criteria APS Manifestations' were to assess the clinical utility of the international consensus statement on classification criteria and treatment guidelines for the catastrophic APS, to identify and grade the studies that analyze the relationship between the antiphospholipid antibodies and the non-criteria APS manifestations, and to present the current evidence regarding the accuracy of these non-criteria APS manifestations for the detection of patients with APS. This article summarizes the studies analyzed on thrombocytopenia and skin manifestations, and presents the recommendations elaborated by the Task Force after this analysis.

  3. AIRE variations in Addison's disease and autoimmune polyendocrine syndromes (APS)

    DEFF Research Database (Denmark)

    Bøe Wolff, A S; Oftedal, B; Johansson, S

    2008-01-01

    Autoimmune Addison's disease (AAD) is often associated with other components in autoimmune polyendocrine syndromes (APS). Whereas APS I is caused by mutations in the AIRE gene, the susceptibility genes for AAD and APS II are unclear. In the present study, we investigated whether polymorphisms...... or copy number variations in the AIRE gene were associated with AAD and APS II. First, nine SNPs in the AIRE gene were analyzed in 311 patients with AAD and APS II and 521 healthy controls, identifying no associated risk. Second, in a subgroup of 25 of these patients, AIRE sequencing revealed three novel...... polymorphisms. Finally, the AIRE copy number was determined by duplex quantitative PCR in 14 patients with APS I, 161 patients with AAD and APS II and in 39 healthy subjects. In two Scandinavian APS I patients previously reported to be homozygous for common AIRE mutations, we identified large deletions...

  4. Conserved Aspartic Acid Residues Lining the Extracellular Loop I of Sodium-coupled Bile Acid Transporter ASBT Interact with Na+ and 7α-OH Moieties on the Ligand Cholestane Skeleton*

    Science.gov (United States)

    Hussainzada, Naissan; Da Silva, Tatiana Claro; Zhang, Eric Y.; Swaan, Peter W.

    2008-01-01

    Functional contributions of residues Val-99—Ser-126 lining extracellular loop (EL) 1 of the apical sodium-dependent bile acid transporter were determined via cysteine-scanning mutagenesis, thiol modification, and in silico interpretation. Despite membrane expression for all but three constructs (S112C, Y117C, S126C), most EL1 mutants (64%) were inactivated by cysteine mutation, suggesting a functional role during sodium/bile acid co-transport. A negative charge at conserved residues Asp-120 and Asp-122 is required for transport function, whereas neutralization of charge at Asp-124 yields a functionally active transporter. D124A exerts low affinity for common bile acids except deoxycholic acid, which uniquely lacks a 7α-hydroxyl (OH) group. Overall, we conclude that (i) Asp-122 functions as a Na+ sensor, binding one of two co-transported Na+ ions, (ii) Asp-124 interacts with 7α-OH groups of bile acids, and (iii) apolar EL1 residues map to hydrophobic ligand pharmacophore features. Based on these data, we propose a comprehensive mechanistic model involving dynamic salt bridge pairs and hydrogen bonding involving multiple residues to describe sodium-dependent bile acid transporter-mediated bile acid and cation translocation. PMID:18508772

  5. Analysis list: CASP8AP2 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available CASP8AP2 Digestive tract + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/CAS...P8AP2.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/CASP8AP2.5.tsv http://dbarchive.bi...osciencedbc.jp/kyushu-u/hg19/target/CASP8AP2.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/CAS

  6. Testing the presence of lithium on the surfaces of cool Ap stars

    Science.gov (United States)

    Nesvacil, N.; Hubrig, S.; Mathys, G.

    2004-12-01

    The possibility of a quite high Li abundance in the Ap stars was first raised by Wallerstein & Merchant (1965). Since then many studies investigated the problem of Li. The more recent observations in the lithium region indicate that in some Ap stars the λ 6708 feature is variable and this variability can be explained by the existence of Li rich spots on the stellar surface. Atomic data for the Ce II λ 6708.099 were released by the D.R.E.A.M. database in 2002. The line was used to identify the prominent suspected Li-feature in post AGB stars and might as well be responsible for the absorption feature in Ap stars. Recent studies have mentioned this possibility, but it has yet to be investigated in more detail. Other physical phenomena, such as the occurrence of a partial Paschen-Back effect in the presence of magnetic fields, as well as possible hyperfine structure splitting of some Rare Earth transitions, must be taken into account to provide correct line identifications in the wavelength region around the Li-doublet at λ 6708. We discuss a possible strategy to clarify the presence of Li in Ap stars.

  7. AP600 containment purge radiological analysis

    Energy Technology Data Exchange (ETDEWEB)

    O`Connor, M.; Schulz, J.; Tan, C. [Bechtel Power Corporation (United States)] [and others

    1995-02-01

    The AP600 Project is a passive pressurized water reactor power plant which is part of the Design Certification and First-of-a-Kind Engineering effort under the Advanced Light Water Reactor program. Included in this process is the design of the containment air filtration system which will be the subject of this paper. We will compare the practice used by previous plants with the AP600 approach to meet the goals of industry standards in sizing the containment air filtration system. The radiological aspects of design are of primary significance and will be the focus of this paper. The AP600 Project optimized the design to combine the functions of the high volumetric flow rate, low volumetric flow rate, and containment cleanup and other filtration systems into one multi-functional system. This achieves a more simplified, standardized, and lower cost design. Studies were performed to determine the possible concentrations of radioactive material in the containment atmosphere and the effectiveness of the purge system to keep concentrations within 10CFR20 limits and within offsite dose objectives. The concentrations were determined for various reactor coolant system leakage rates and containment purge modes of operation. The resultant concentrations were used to determine the containment accessibility during various stages of normal plant operation including refueling. The results of the parametric studies indicate that a dual train purge system with a capacity of 4,000 cfm per train is more than adequate to control the airborne radioactivity levels inside containment during normal plant operation and refueling, and satisfies the goals of ANSI/ANS-56.6-1986 and limits the amount of radioactive material released to the environment per ANSI/ANS 59.2-1985 to provide a safe environment for plant personnel and offsite residents.

  8. Ozone mitigation tests at the APS

    Energy Technology Data Exchange (ETDEWEB)

    Kuzay, T.M.; Collins, J.T.; Pisharody, M.; Job, P.K.; Wang Zhibi

    1996-09-01

    Ozone is generated in the APS experimental stations whenever the x-ray beam has a chance to interact with air. Ozone concentrations in an experimental station have to be below a certain defined limit (current OSHA regulations specify 0.08 ppm as the maximum limit) before an experimenter can reenter the hutch. This limit is said to be currently under study for a downward adjustment. One method of depleting the ozone generated in an experimental station is mitigation through either adsorption or direct destruction. In recent tests, both methods were tried using commercially available units. Test results and some analytical predictions are presented.

  9. Wiley AP english language and composition

    CERN Document Server

    Woods, Geraldine

    2013-01-01

    Two full-length practice tests to help you score your highest Review questions with complete answers and explanations for the most popular themes on the test Study strategies and expert test-taking tips to keep you calm and focused on test day Review key exam topics and themes to maximize your score Preparing for the AP English Language and Composition test? This test-prep guide helps you perfect the skills you need to achieve your best possible score. You'll find winning test-taking tips, multiple-choice strategies, and essay guidelines, as well as great advice on getting the

  10. Thioredoxin reductase regulates AP-1 activity as well as thioredoxin nuclear localization via active cysteines in response to ionizing radiation.

    Science.gov (United States)

    Karimpour, Shervin; Lou, Junyang; Lin, Lilie L; Rene, Luis M; Lagunas, Lucio; Ma, Xinrong; Karra, Sreenivasu; Bradbury, C Matthew; Markovina, Stephanie; Goswami, Prabhat C; Spitz, Douglas R; Hirota, Kiichi; Kalvakolanu, Dhananjaya V; Yodoi, Junji; Gius, David

    2002-09-12

    A recently identified class of signaling factors uses critical cysteine motif(s) that act as redox-sensitive 'sulfhydryl switches' to reversibly modulate specific signal transduction cascades regulating downstream proteins with similar redox-sensitive sites. For example, signaling factors such as redox factor-1 (Ref-1) and transcription factors such as the AP-1 complex both contain redox-sensitive cysteine motifs that regulate activity in response to oxidative stress. The mammalian thioredoxin reductase-1 (TR) is an oxidoreductase selenocysteine-containing flavoprotein that also appears to regulate multiple downstream intracellular redox-sensitive proteins. Since ionizing radiation (IR) induces oxidative stress as well as increases AP-1 DNA-binding activity via the activation of Ref-1, the potential roles of TR and thioredoxin (TRX) in the regulation of AP-1 activity in response to IR were investigated. Permanently transfected cell lines that overexpress wild type TR demonstrated constitutive increases in AP-1 DNA-binding activity as well as AP-1-dependent reporter gene expression, relative to vector control cells. In contrast, permanently transfected cell lines expressing a TR gene with the active site cysteine motif deleted were unable to induce AP-1 activity or reporter gene expression in response to IR. Transient genetic overexpression of either the TR wild type or dominant-negative genes demonstrated similar results using a transient assay system. One mechanism through which TR regulates AP-1 activity appears to involve TRX sub-cellular localization, with no change in the total TRX content of the cell. These results identify a novel function of the TR enzyme as a signaling factor in the regulation of AP-1 activity via a cysteine motif located in the protein.

  11. The novel mitochondria-targeted hydrogen sulfide (H2S) donors AP123 and AP39 protect against hyperglycemic injury in microvascular endothelial cells in vitro.

    Science.gov (United States)

    Gerő, Domokos; Torregrossa, Roberta; Perry, Alexis; Waters, Alicia; Le-Trionnaire, Sophie; Whatmore, Jacqueline L; Wood, Mark; Whiteman, Matthew

    2016-11-01

    The development of diabetic vascular complications is initiated, at least in part, by mitochondrial reactive oxygen species (ROS) production in endothelial cells. Hyperglycemia induces superoxide production in the mitochondria and initiates changes in the mitochondrial membrane potential that leads to mitochondrial dysfunction. Hydrogen sulfide (H2S) supplementation has been shown to reduce the mitochondrial oxidant production and shows efficacy against diabetic vascular damage in vivo. However, the half-life of H2S is very short and it is not specific for the mitochondria. We have therefore evaluated two novel mitochondria-targeted anethole dithiolethione and hydroxythiobenzamide H2S donors (AP39 and AP123 respectively) at preventing hyperglycemia-induced oxidative stress and metabolic changes in microvascular endothelial cells in vitro. Hyperglycemia (HG) induced significant increase in the activity of the citric acid cycle and led to elevated mitochondrial membrane potential. Mitochondrial oxidant production was increased and the mitochondrial electron transport decreased in hyperglycemic cells. AP39 and AP123 (30-300nM) decreased HG-induced hyperpolarisation of the mitochondrial membrane and inhibited the mitochondrial oxidant production. Both H2S donors (30-300nM) increased the electron transport at respiratory complex III and improved the cellular metabolism. Targeting H2S to mitochondria retained the cytoprotective effect of H2S against glucose-induced damage in endothelial cells suggesting that the molecular target of H2S action is within the mitochondria. Mitochondrial targeting of H2S also induced >1000-fold increase in the potency of H2S against hyperglycemia-induced injury. The high potency and long-lasting effect elicited by these H2S donors strongly suggests that these compounds could be useful against diabetic vascular complications.

  12. Trichomonas vaginalis: the adhesins AP51 and AP65 bind heme and hemoglobin.

    Science.gov (United States)

    Ardalan, Shahed; Lee, B Craig; Garber, Gary E

    2009-04-01

    Trichomonas vaginalis is the cause of human trichomoniasis, the most common non-viral sexually transmitted disease worldwide. Although acquisition of iron by binding to host hemoglobin through distinct receptor(s) has been described, no specific heme- or hemoglobin-binding site has been reported in this parasite. To determine the presence of hemoglobin-binding protein(s), membrane proteins were subjected to hemoglobin-affinity chromatography. Eluted proteins were analysed by SDS-PAGE. Two protein bands of 48 and 63 kDa were detected. Competition assay with an excess amount of hemoglobin or hemin in hemoglobin-affinity chromatography could block the 63- and 48-kDa bands, respectively. Further analysis by mass spectrometry indicated that the 48- and 63-kDa proteins had identity with two T. vaginalis adhesins: AP51 and AP65, respectively. This study confirms the existence of multifunctional proteins in T. vaginalis, and suggested that AP51 and AP65, besides serving as adhesion molecules, could also act as heme- and hemoglobin-binding proteins.

  13. AP1000进度管理研究%The study on AP1000 Schedule management

    Institute of Scientific and Technical Information of China (English)

    李阳; 王飞鸣

    2015-01-01

    核电建设进度管理非常重要,本文针对核电站进度管理的内容、体系、管理方式等进行介绍,并结合AP1000核电技术的特点——模块化,分析其对进度管理的影响和目前存在的问题。并结合影响和问题提出了在后续AP1000建设进度管理中的几项建议。%Nuclear power plant construction progress management is very important. This paper addresses important topics including the content, system and manage methods of nuclear power plants construction. Combining the characteristics of AP1000 nuclear power technology-modular, the paper analyzed its impact on progress management and the existing problems. Depending on the impacts and problems, the paper also raised several suggestions in AP1000 modular construction.

  14. Transmission Lines or Poles, Electric, MDTA High Mast lighting, High Mast Lighting along I 95, Maryland Transportation Authority High Mast Lighting poles, Published in 2011, 1:1200 (1in=100ft) scale, Maryland Transportation Authority.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Transmission Lines or Poles, Electric dataset, published at 1:1200 (1in=100ft) scale, was produced all or in part from Orthoimagery information as of 2011. It...

  15. Proximal femoral canal shape is more accurately assessed on AP hip radiographs than AP pelvis radiographs in primary hip osteoarthritis.

    Science.gov (United States)

    Merle, Christian; Waldstein, Wenzel; Gregory, Jennifer S; Goodyear, Simon R; Aspden, Richard M; Aldinger, Peter R; Murray, David W; Gill, Harinderjit S

    2013-01-01

    The objectives of the present study were to determine whether differences in the radiographic appearance of the of the proximal femoral canal exist on corresponding AP pelvis and AP hip radiographs, and whether radiographic assessment of canal shape is accurate with reference to computed tomography (CT). In a retrospective study, corresponding radiographs and CT scans of 100 consecutive patients with primary hip OA were evaluated. Active shape modelling (ASM) was performed to assess the variation in proximal femoral canal shape and to identify differences between AP hip and AP pelvis views. Differences in the medial cortical flare between radiographs and CT were quantified using least squares curve fitting. ASM identified significant differences in the assessment of canal shape on corresponding AP hip and AP pelvis views. Curve fitting demonstrated a good agreement between AP hip radiographs and CT. Agreement between AP pelvis radiographs and CT was less good. In contrast to AP pelvis radiographs, AP hip radiographs allow a more accurate and reliable assessment of proximal femoral canal shape in the frontal plane in primary hip OA. Our findings may improve stem fit in total hip arthroplasty without the routine use of CT.

  16. Specific activation of human interleukin-5 depends on de novo synthesis of an AP-1 complex.

    Science.gov (United States)

    Schwenger, Gretchen T F; Kok, Chee Choy; Arthaningtyas, Estri; Thomas, Marc A; Sanderson, Colin J; Mordvinov, Viatcheslav A

    2002-12-06

    It is clear from the biology of eosinophilia that a specific regulatory mechanism must exist. Because interleukin-5 (IL5) is the key regulatory cytokine, it follows that a gene-specific control of IL5 expression must exist that differs even from closely related cytokines such as IL4. Two features of IL5 induction make it unique compared with other cytokines; first, induction by cyclic adenosine monophosphate (cAMP), which inhibits other T-cell-derived cytokines, and second, sensitivity to protein synthesis inhibitors, which have no effect on other cytokines. This study has utilized the activation of different transcription factors by different stimuli in a human T-cell line to study the role of conserved lymphokine element 0 (CLE0) in the specific induction of IL5. In unstimulated cells the ubiquitous Oct-1 binds to CLE0. Stimulation induces de novo synthesis of the AP-1 members JunD and Fra-2, which bind to CLE0. The amount of IL5 produced correlates with the production of the AP-1 complex, suggesting a key role in IL5 expression. The formation of the AP-1 complex is essential, but the rate-limiting step is the synthesis of AP-1, especially Fra-2. This provides an explanation for the sensitivity of IL5 to protein synthesis inhibitors and a mechanism for the specific induction of IL5 compared with other cytokines.

  17. Analyzing the Influence of Constructing Daxi Passenger Transport Railway Line on Water Environment of Lancun Spring-feeding Area%大西客运专线建设对兰村泉域水环境影响分析

    Institute of Scientific and Technical Information of China (English)

    陈珊

    2012-01-01

    从站点给排水、隧道建设、桥梁建设、路基建设、站场建设、电气化建设和铁路运营等几个方面分析大西客专施工与运营对兰村泉域水环境的影响。%The paper analyzes the influence of constructing Daxi passenger transport railway line on water environment of Lancun spring-feeding area from several aspects: railway station drainage,tunnel construction,bridge construction,railway base construction,station construction,electrification construction and railway operation.

  18. Project W-211, initial tank retrieval systems, description of operations for 241-AP-102 and 241-AP-104

    Energy Technology Data Exchange (ETDEWEB)

    RIECK, C.A.

    1999-02-25

    The primary purpose of the Initial Tank Retrieval Systems (ITRS) is to provide systems for retrieval of radioactive wastes stored in underground double-shell tanks (DSTS) for transfer to alternate storage, evaporation, pretreatment or treatment, while concurrently reducing risks associated with safety watch list and other DSTs. This Description of Operations (DOO) defines the control philosophy for the waste retrieval system for tanks 241-AP-102 (AP-102) and 241-AP-104 (AP-104). This DOO will provide a basis for the detailed design of the Retrieval Control System (RCS) for AP-102 and AP-104 and establishes test criteria for the RCS. The test criteria will be used during qualification testing and acceptance testing to verify operability.

  19. AP1000主系统先进性分析%The Progressiveness Analysis of the AP1000 Main System

    Institute of Scientific and Technical Information of China (English)

    李家杰

    2012-01-01

      本文总结了第三代先进非能动压水堆核电厂AP1000主系统的主要特点,对AP1000主系统的先进性进行了分析,核电厂主系统设计人员能够从该文中了解到 AP1000主系统与第二代核电厂主系统之间的差别。%  This paper summarizes the main features of the AP1000 main system of the third generation of advanced passive pressurized water reactor nuclear power plant , and analyzes the progressiveness of the AP1000 main system .From this paper, the main system designers of nuclear power factory can learn the difference between the second-generation nuclear power factory main system and AP1000 main system

  20. New Beam Position Monitor System Design for the APS Injector

    Science.gov (United States)

    Lill, R.; Singh, O.; Arnold, N.

    2002-12-01

    Demands on the APS injector have evolved over the last few years to the point that an upgrade to the existing beam position monitor (BPM) electronics is required. The injector is presently being used as a source for both the low-energy undulator test line (LEUTL) project and the top-up mode of operation. These new requirements and the fact that many new rf receiver components are available at reasonable cost make this upgrade very desirable at this time. The receiver topology selected is a logarithmic processor, which is designed around the Analog Devices AD8313 log amplifier demodulation chip. This receiver will become the universal replacement for all injector applications measuring positions signals from 352 to 2856 MHz with minimum changes in hardware and without the use of a downconverter. The receiver design features integrated front-end gain and built-in self test. The data acquisition being considered at this time is a 100-MHz, 12-bit transient recorder digitizer. The latest experimental and commissioning data and results will be presented.

  1. Top-up operation experience at APS.

    Energy Technology Data Exchange (ETDEWEB)

    Emery, L.

    1999-03-31

    The Advanced Photon Source (APS) is a 7-OeV, third-generation synchrotrons radiation source. To provide more stable beam for users, in September 1998 we began commissioning a new operating mode called ''top-up.'' In this mode, the beam current does not decay but is maintained at a high level using frequent injection, while photon shutters are open and photon beams are delivered to users. The hardware, software, and safety requirements for top-up will be reported. Safety issues related to injection with open photon shutters are covered in companion papers in this conference. Recent operational experience includes testing aspects of top-up injection and delivering beam to X-ray users for a few hours with fractional current stability of 10{sup {minus}3}. We expect to run several top-up operation shifts in Spring 1999. Issues of importance are orbit and emittance transients during the injection and scheduling of injection pulses for the convenience of users.

  2. AP233: An Information Model for Systems Engineering

    Science.gov (United States)

    Siebes, Georg

    2009-01-01

    In today's world, information is abundant. We have no problems generating it. But we are challenged to find, organize, and exchange information. center dot A standardized model of information can help. Such a model nearly completed its development for Systems Engineering. It is referred to as AP233 (AP = Application Protocol).

  3. APS extends open access to all its journals

    CERN Multimedia

    Thomas, Kim

    2006-01-01

    "Physics research promoter and publisher the American Physical Society (APS) is to extend open access to all its journals. Th APS previously made its five print journals available through subscriptions, and its two e-journals (Physical Review Special Topics and Physics Educatoin Research) on an open access basis." (1/2 page)

  4. Native Mobile Ap or Mobile Web Page?

    OpenAIRE

    2013-01-01

    This project is designed to greatly enhance the lines of communication between the pollinator industry and the pesticide applicator community. It builds on the DriftWatch.org Pesticide Sensitive Crop Registry (www.driftwatch.org), which currently covers nine states, with a customized map for each state (Colorado, Indiana, Illinois, Michigan, Missouri, Minnesota, Montana, Nebraska, and Wisconsin).

  5. Continuous on-line calibration of diffusive soil-atmosphere trace gas transport using vertical {sup 220}Rn- and {sup 222}Rn-activity profiles

    Energy Technology Data Exchange (ETDEWEB)

    Lehmann, B.E. [Bern Univ. (Switzerland). Physics Inst.; Neftel, A. [Inst. of Environmental Protection and Agriculture, Bern (Switzerland); Tarakanov, S.V. [Inst. of Silicate Chemistry, St. Petersburg (Russian Federation)

    2001-07-01

    Continuous monitoring of {sup 220}Rn- and {sup 222}Rn-activities above and below the soil surface combined with sporadic direct {sup 222}Rn-flux measurements is used to quantify diffusive trace gas transport in the air-filled pore space of soil, through the soil-atmosphere interface and in the lowest layers of the atmosphere. In a calm night, {sup 222}Rn-activities above the surface first build-up near the ground (z < 10 cm) and subsequently with a delay of 2-3 hours at higher altitudes (z < 5 m). Knowing (1) the {sup 222}Rn-flux from activity profiles measured in soil gas, (2) from direct flux determinations and (3) using information about atmospheric diffusion parameters from {sup 220}Rn-activities measured near the surface it is possible to model the temporal evolution of the vertical {sup 222}Rn-profiles in a night with stable weather and constant soil conditions. The system operates automatically for extended periods of time in the field enabling a better understanding of transport processes in response to changing environmental conditions (wind, rain, soil humidity). (orig.)

  6. Different involvement of promoter methylation in the expression of organic cation/carnitine transporter 2 (OCTN2 in cancer cell lines.

    Directory of Open Access Journals (Sweden)

    Qiang Qu

    Full Text Available Organic cation/carnitine transporter 2 (OCTN2 is responsible for the cellular uptake of the antineoplastic agent, oxaliplatin. Epigenetic modification is a possible mechanism of altered drug-transporter expression in cancers, leading to altered efficacy of chemotherapeutic drugs. However, the mechanisms governing OCTN2 regulation are not completely understood. In this study, the low levels of OCTN2 in HepG2 and LS174T cells were elevated by the demethylating reagent, decitabine (DCA. To further reveal the epigenetic mechanism of down-regulation of OCTN2, we found that Region-1 within the OCTN2 promoter (spanning -354 to +85 was a determinant of OCTN2 expression in a luciferase reporter assay. Moreover, methylation-specific PCR (MSP and bisulfite genomic sequencing showed that the degree of individual methylated CpG sites within this region was inversely correlated with the levels of OCTN2 in different cancer cells. Application of DCA to HepG2 and LS174T cells reversed the hypermethylation status of the OCTN2 promoter and increased OCTN2 expression, enhancing cellular uptake of oxaliplatin. Thus, we identified that promoter methylation is responsible for epigenetic down-regulation of OCTN2 in HepG2 and LS174T cells. Given the essential role of OCTN2 in cancer cell uptake of chemotherapeutics, and thus treatment efficacy, pretreatment with a demethylating reagent is a possible strategy for optimizing pharmacotherapies against cancers.

  7. Evaluation and Modification of NOTRUMP-AP600 Code With Neglecting Momentum Flux Terms%对 NOTRUMP-AP600程序缺少动量通量项的评估与修正

    Institute of Scientific and Technical Information of China (English)

    樊普; 郑尧瑶

    2014-01-01

    The momentum flux terms are not included in the momentum equations for NOTRUMP-AP600 code .The predicted injection mass flow rate of IRWST and pres-surizer level deviate test results largely when NOTRUMP-AP600 code is used to analyze SBLOCA for APEX-600 test facility . The impact of neglecting the momentum flux terms in the NOTRUMP-AP600 was evaluated ,including the following contents :The impact of neglecting momentum flux terms for the pressure drop of ADS line was calcu-lated using homogenous model and separated flow model ;the detailed two-phase pres-sure drop was calculated using FLOAD4 code for the ADS4 line ,and the result was used to benchmark the ADS4 pressure drop for NOTRUMP-AP600 .The results show that the input resistance coefficient should be increased by 60% for AP600 ADS4 line .%由于NOTRUMP-AP600程序的动量守恒方程缺少动量通量项,在分析用于模拟AP600核电厂的A PEX试验台架小破口事故时,安全壳内置换料水箱注射流量和稳压器混合水位等参数的预测值和实验值有较大偏离。本文对此进行评估:1)采用均相流和分相流模型计算动量通量项对A P600核电厂自动卸压系统(ADS)管路压降的影响;2)采用FLOAD4程序对需修正的第4级ADS(ADS4)管路的两相流压降进行计算,预测ADS4管路内的压力分布,并用作修正NOTRUMP-AP600程序 ADS4管路压降的基准。结果表明,对于A P600核电厂ADS4管路,输入阻力系数需增加60%。

  8. The effect of the plasticizer diethylhexyl phthalate on transport activity and expression of P-glycoprotein in parental and doxo-resistant human sarcoma cell lines.

    Science.gov (United States)

    Angelini, A; Centurione, L; Sancilio, S; Castellani, M L; Conti, P; Di Ilio, C; Porreca, E; Cuccurullo, F; Di Pietro, R

    2011-01-01

    Multidrug resistance (MDR) to cancer therapy is frequently associated with the over-expression of the multidrug transporter MDR1 gene product P-glycoprotein (Pgp) in several types of human tumours. Various chemosensitizers have been used to inhibit Pgp activity but toxicity limits their clinical application. Di(2-ethylhexyl)phthalate (DEHP) is a plasticizer that is released from polyvinyl chloride (PVC) medical devices. Therefore, cancer patients undertaking chemotherapy are exposed to a clinically important amount of DEHP through blood and blood component transfusions, apheresis products, intravenous chemotherapy, parenteral nutrition and other medical treatments. The present study was designed to investigate the effects of DEHP on transport activity and expression of Pgp in order to evaluate its potential use as a chemosensitizer in cancer therapy. Human doxorubicin (doxo) resistant sarcoma cells (MES-SA/Dx5) that over-express Pgp were treated with different doses of doxo (2, 4 and 8 μM) in the presence or absence of various concentrations of DEHP (3, 6 and 12 μM) that were clinically achievable in vivo. Our results show that co-treatment with 2, 4 and 8 μM doxo in the presence of the lowest concentration of DEHP (3 μM) enhanced significantly doxo accumulation in MES-SA/Dx5 cells and, consistently increased the sensitivity to doxo, when compared to controls receiving only doxo. In contrast, higher DEHP concentrations (6 and 12 μM) induced MES-SA/Dx5 to extrude doxo decreasing doxo cytotoxicity toward resistant cells below control values. These results are consistent with the increase in Pgp expression levels in parental MES-SA cells treated with 3, 6 and 12 μM DEHP for 24 h and compared to untreated controls. All in all, these findings suggest a potential clinical application of DEHP as a chemosensitizer to improve effectiveness of the antineoplastic drugs in MDR human tumours.

  9. Comparative evaluation of transport mechanisms of trans-1-amino-3-[¹⁸F]fluorocyclobutanecarboxylic acid and L-[methyl-¹¹C]methionine in human glioma cell lines.

    Science.gov (United States)

    Ono, Masahiro; Oka, Shuntaro; Okudaira, Hiroyuki; Schuster, David M; Goodman, Mark M; Kawai, Keiichi; Shirakami, Yoshifumi

    2013-10-16

    Positron emission tomography (PET) with amino acid tracers is useful for the visualization and assessment of therapeutic effects on gliomas. Our purpose is to elucidate the transport mechanisms of trans-1-amino-3-[¹⁸F]fluorocyclobutanecarboxylic acid (anti-[¹⁸F]FACBC) and L-[methyl-¹¹C]methionine ([¹¹C]Met) in normal human astrocytes (NHA), low-grade (Hs683, SW1088), and high-grade (U87MG, T98G) human glioma cell lines. Because the short half-lives of fluorine-18 and carbon-11 are inconvenient for in vitro experiments, trans-1-amino-3-fluoro[1-¹⁴C]cyclobutanecarboxylic acid (anti-[¹⁴C]FACBC) and L-[methyl-¹⁴C]methionine ([¹⁴C]Met) were used instead of the PET tracers. Time-course uptake experiments showed that uptake of anti-[¹⁴C]FACBC was 1.4-2.6 times higher than that of [¹⁴C]Met in NHA and low-grade glioma cells, and was almost equal to that of [¹⁴C]Met in high-grade glioma cells. To identify the amino acid transporters (AATs) involved in the transport of anti-[¹⁴C]FACBC and [¹⁴C]Met, we carried out competitive inhibition experiments using synthetic/naturally-occurring amino acids as inhibitors. We found that anti-[¹⁴C]FACBC uptake in the presence of Na⁺ was strongly inhibited by L-glutamine and L-serine (the substrates for ASC system AATs), whereas L-phenylalanine and 2-amino-bicyclo[2,2,1]heptane-2-carboxylic acid (BCH, the substrates for L system AATs) robustly inhibited Na⁺-independent anti-[¹⁴C]FACBC uptake. Regardless of Na⁺, [¹⁴C]Met uptake was inhibited strongly by L-phenylalanine and BCH. Moreover, the exchange transport activity of L-glutamine for anti-[¹⁴C]FACBC was stronger than that of BCH in the presence of Na⁺, whereas that for [¹⁴C]Met was almost equal to BCH. These results demonstrate that ASC and L are important transport systems for anti-[¹⁸F]FACBC uptake, while system L is predominantly involved in [¹¹C]Met transport in human astrocytes and glioma cells.

  10. Total on-line monitoring system of Tokyo gas transmission pipelines; Systeme global de controle et de surveillance des canalisations de transport du gaz developpe par Tokyo gas

    Energy Technology Data Exchange (ETDEWEB)

    Shibata, M. [Tokyo Gas Co., Ltd (Japan)

    2000-07-01

    As Tokyo Gas transmission pipeline is located in residential areas of Metropolitan Tokyo, more precise and advanced maintenance and inspection methods become necessary. A more efficient maintenance and inspection management system is being sought in line with the extension of gas transmission pipelines. Research and development is underway for various types of maintenance /monitoring systems that predict or detect pipeline damage or failure. Some systems have already been put to practical use. Tokyo Gas has developed a total online monitoring system featuring upgraded performance and centralized data processing. This system carries out 24-hour monitoring for damage and failure, and sends warnings to operators at the Pipeline Regional Network Office. This paper introduces the functions of the system, as well as the functions which are currently in the R and D stage. (author)

  11. Tobacco carcinogen mediated up-regulation of AP-1 dependent pro-angiogenic cytokines in head and neck carcinogenesis.

    Science.gov (United States)

    Swenson, Wade G; Wuertz, Beverly R K; Ondrey, Frank G

    2011-09-01

    Tobacco is notably genotoxic and associated with head and neck carcinogenesis. Cigarette carcinogens have the capacity to alter early response gene expression in tobacco-related malignancies via genes such as nuclear factor kappa B (NFκB). A number of early response gene activation events are also facilitated by fos/jun activator protein 1 (AP-1) associated pathways. In the present study, we hypothesize that tobacco products may induce microenvironment alterations, promoting angiogenesis and providing a permissive environment for head and neck cancer progression. In an in vitro analysis, we employed immortalized oral keratinocyte (HOK-16B) and laryngeal squamous carcinoma (UM-SCC-11A) cells to investigate interleukin (IL)-8 and vascular endothelial growth factor (VEGF) induction by cigarette smoke condensate (CSC). IL-8 and VEGF expression is based on interactions between NFκB, AP-1, and NF-IL6. We identified at least 1.5-fold dose-dependent induction of AP-1, VEGF, and IL-8 promoter/reporter gene activity after 24 h exposure to CSC. Next, we stably transfected UM-SCC-11A cells with A-Fos, a dominant negative AP-1 protein. Treatment with CSC of the A-Fos cell lines compared to empty vector controls significantly down-regulated AP-1, VEGF, and IL-8 promoter/reporter gene expression. We also performed ELISAs and discovered significant up-regulation of IL-8 and VEGF secretion by UMSCC 11A after treatment with phorbol 12-myristate 13-acetate, tumor necrosis factor alpha, and CSC, which was down-regulated by the A-Fos dominant negative protein. We conclude tobacco carcinogens up-regulate AP-1 activity and AP-1 dependent IL-8 and VEGF gene expression in head and neck cancer. This up-regulation may promote an angiogenic phenotype favoring invasion in both premalignant and squamous cancer cells of the head and neck.

  12. AP600 design certification thermal hydraulics testing and analysis

    Energy Technology Data Exchange (ETDEWEB)

    Hochreiter, L.E.; Piplica, E.J.

    1995-09-01

    Westinghouse Electric Corporation, in conjunction with the Department of Energy and the Electric Power Research Institute, have been developing an advanced light water reactor design; the AP600. The AP600 is a 1940 Mwt, 600Mwe unit which is similar to a Westinghouse two-loop Pressurized Water Reactor. The accumulated knowledge on reactor design to reduce the capital costs, construction time, and the operational and maintenance cost of the unit once it begins to generate electrical power. The AP600 design goal is to maintain an overall cost advantage over fossil generated electrical power.

  13. A new gap separation mechanism for APS insertion devices.

    Energy Technology Data Exchange (ETDEWEB)

    Trakhtenberg, E. M.; Tcheskidov, V.; Den Hartog, P. K.; Deriy, B.; Erdmann, M.; Makarov, O.; Moog, E. R.

    1999-10-25

    A new gap separation mechanism for use with the standard Advanced Photon Source (APS) 3.3-cm-period undulator magnetic structures has been designed and built and the first system has been installed in the APS storage ring. The system allows a minimum magnetic gap of 10 mm for use with the APS 8-mm insertion device vacuum chambers. The mechanism is a bolted steel frame structure with a simple 4-motor mechanical drive train. The control system uses servomotors with incremental rotary encoders and virtual absolute linear encoders.

  14. C. Petrone et al.: "Magnetic measurement of the model magnet QD0 designed for the CLIC final focus beam transport line." CERN TE-MSC Internal Note, EDMS Nr: 1184196

    CERN Document Server

    Arpaia, Pasquale; Petrone, Carlo; Russenschuck, Stephan; Walckiers, Louis

    2012-01-01

    This note presents the results of the magnetic measurements performed on QD0, model magnet for the final focus transport line for CLIC (Fig. 1). This high-gradient, hybrid quadrupole has a yoke length of 0.1 m and an aperture of 8.3 mm. ND2Fe14B Permanent magnet blocks provide a gradient of 150 T/m, which can be further increased to 530 T/m when the four coils are excited to 18.3 A. The request was to measure the strength of the field and the multipole coefficients at different currents. The measurement of the field strength, by means of the single stretched wire system, was done in December 2011 in the I8 laboratory. The measurement of the multipole was done by means of the oscillating wire system [1][2].

  15. 上海轨道交通8号线双休日运营组织模式优化%Optimization of Weekend Passenger Transport Organization on Shanghai Rail Transit Line 8

    Institute of Scientific and Technical Information of China (English)

    胡威; 陆志坚

    2016-01-01

    Shanghai metro Line 8 is a rapid N-S traffic aisle,it undertakes heavy passenger transport task every day.Because of the design deficiencies in early stage and rapid urban development,requests for present transport or-ganization mode keep on increasing. So, the operating company should consider the special and timely demands of passengers at different time periods to promote the opera-tion quality and service level after meeting the needs of dai-ly work.In this paper,some optimization plans to enhance operation quality based on weekend transit and passenger features,also on the space-time distribution analysis of Line 8 are proposed.%上海轨道交通8号线作为一条南北走向的城市快速通道,每天承担着繁重的客运任务。但由于先期设计缺陷及后期城市发展,给当下的日常运营组织提出了多方面的运营需求。作为运营企业在满足日常运量需求的基础上,更应积极考虑不同时段客运需求的特殊性与时效性,从而提升运营企业的运营质量及服务品质。针对当下8号线节假日运营客流出行特点及分布时空提出了运营组织优化方案,以提升运营质量。

  16. Modelling the incomplete Paschen-Back effect in the spectra of magnetic Ap stars

    OpenAIRE

    Stift, M. J.; Leone, F.; Degl'Innocenti, E. Landi

    2008-01-01

    We present first results of a systematic investigation of the incomplete Paschen-Back effect in magnetic Ap stars. A short overview of the theory is followed by a demonstration of how level splittings and component strengths change with magnetic field strength for some lines of special astrophysical interest. Requirements are set out for a code which allows the calculation of full Stokes spectra in the Paschen-Back regime and the behaviour of Stokes I and V profiles of transitions in the mult...

  17. Analysis of an AP600 intermediate-size loss-of-coolant accident

    Energy Technology Data Exchange (ETDEWEB)

    Boyack, B.E.; Lime, J.F. [Los Alamos National Lab., NM (United States)

    1995-09-01

    A postulated double-ended guillotine break of an AP600 direct-vessel-injection line has been analyzed. This event is characterized as an intermediate-break loss-of-coolant accident. Most of the insights regarding the response of the AP600 safety systems to the postulated accident are derived from calculations preformed with the TRAC-PF1/MOD2 code. However, complementary insights derived from a scaled experiment conducted in the ROSA facility, as well as insights based upon calculations by other codes, are also presented. Based upon the calculated and experimental results, the AP600 will not experience a core heat up and will reach a safe shutdown state using only safety-class equipment. Only the early part of the long-term cooling period initiated by In-containment Refueling Water Storage Tank injection was evaluated. Thus, the observation that the core is continuously cooled should be verified for the later phase of the long-term cooling period when sump injection and containment cooling processes are important.

  18. Distinct Temporal Regulation of RET Isoform Internalization: Roles of Clathrin and AP2.

    Science.gov (United States)

    Crupi, Mathieu J F; Yoganathan, Piriya; Bone, Leslie N; Lian, Eric; Fetz, Andrew; Antonescu, Costin N; Mulligan, Lois M

    2015-11-01

    The RET receptor tyrosine kinase (RTK) contributes to kidney and nervous system development, and is implicated in a number of human cancers. RET is expressed as two protein isoforms, RET9 and RET51, with distinct interactions and signaling properties that contribute to these processes. RET isoforms are internalized from the cell surface into endosomal compartments in response to glial cell line-derived neurotropic factor (GDNF) ligand stimulation but the specific mechanisms of RET trafficking remain to be elucidated. Here, we used total internal reflection fluorescence (TIRF) microscopy to demonstrate that RET internalization occurs primarily through clathrin coated pits (CCPs). Activated RET receptors colocalize with clathrin, but not caveolin. The RET51 isoform is rapidly and robustly recruited to CCPs upon GDNF stimulation, while RET9 recruitment occurs more slowly and is less pronounced. We showed that the clathrin-associated adaptor protein complex 2 (AP2) interacts directly with each RET isoform through its AP2 μ subunit, and is important for RET internalization. Our data establish that interactions with the AP2 complex promote RET receptor internalization via clathrin-mediated endocytosis but that RET9 and RET51 have distinct internalization kinetics that may contribute to differences in their biological functions.

  19. Magnetic Doppler imaging of the roAp star HD 24712

    CERN Document Server

    Lüftinger, T; Ryabchikova, T; Piskunov, N; Weiss, W W; Ilyin, I

    2009-01-01

    We present the first magnetic Doppler images of a rapidly oscillating Ap (roAp) star. We deduce information about magnetic field geometry and abundance distributions of a number of chemical elements on the surface of the hitherto best studied roAp star, HD 24712, using the magnetic Doppler imaging (MDI) code, INVERS10, which allows us to reconstruct simultaneously and consistently the magnetic field geometry and elemental abundance distributions on a stellar surface. For this purpose we analyse time series spectra obtained in Stokes I and V parameters with the SOFIN polarimeter at the Nordic Optical Telescope and recover surface abundance structures of sixteen different chemical elements, respectively ions, including Mg, Ca, Sc, Ti, Cr, Fe, Co, Ni, Y, La, Ce, Pr, Nd, Gd, Tb, and Dy. For the rare earth elements (REE) Pr and Nd separate maps were obtained using lines of the first and the second ionization stage. We find and confirm a clear dipolar structure of the surface magnetic field and an unexpected correl...

  20. Effect of multifunctional protein YB-1 on the AP site cleavage by AP endonuclease 1 and tyrosyl phosphodiesterase 1

    Directory of Open Access Journals (Sweden)

    Ovchinnikov L. P.

    2012-07-01

    Full Text Available Apurinic/apyrimidinic sites (AP sites which represent one of the most abundantly generated DNA lesions in the cell are generally repaired by base excision repair (BER pathway. Multifunctional protein YB-1 is known to participate in cellular response to genotoxic stress and was shown to interact with several components of BER – DNA glycosylases NTH1, NEIL2, DNA polymerase and DNA ligase III. Therefore, it is of great interest to investigate the influence of YB-1 on one of the major BER enzymes, responsible for AP site cleavage, AP endonuclease APE1, and on tyrosyl phosphodiesterase Tdp1, participating in APE1 independent pathway of AP site repair. Aim. Effect of multifunctional protein YB-1 on the AP site cleavage by the activities of APE1 and Tdp1 was studied. Methods. Gel-mobility shift assays and enzyme activity tests. Results. YB-1 was shown to inhibit the cleavage of AP site located in single-stranded DNA by both APE1 and Tdp1. Stimulation of APE1 activity on protruding double-stranded DNA in the presence of YB-1 was observed, whereas no effect on Tdp1-mediated cleavage of AP site in double-stranded DNA was found. Conclusions. YB-1 can modulate the repair of AP sites in DNA by both positively stimulating APE1 during the classic BER of AP sites and avoiding a possible generation of doublestrand breaks, arising from the cleavage of single-stranded portion of DNA substrate already used by different DNA-processing pathway

  1. 49 CFR 192.707 - Line markers for mains and transmission lines.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Line markers for mains and transmission lines. 192... § 192.707 Line markers for mains and transmission lines. (a) Buried pipelines. Except as provided in... buried main and transmission line: (1) At each crossing of a public road and railroad; and (2)...

  2. 75 FR 14259 - Transportation Conformity Rule PM2.5

    Science.gov (United States)

    2010-03-24

    ... Protection Agency 40 CFR Part 93 Transportation Conformity Rule PM2.5 and PM10 Amendments; Final Rule #0;#0...; ] ENVIRONMENTAL PROTECTION AGENCY 40 CFR Part 93 RIN 2060-AP29 Transportation Conformity Rule PM 2.5 and PM 10... amending the transportation conformity rule to finalize provisions that were proposed on May 15,...

  3. 75 FR 49435 - Transportation Conformity Rule Restructuring Amendments

    Science.gov (United States)

    2010-08-13

    ... AGENCY 40 CFR Part 93 RIN 2060-AP57 Transportation Conformity Rule Restructuring Amendments AGENCY... restructure several sections of the transportation conformity rule so that they would apply to any new or... in implementing the transportation conformity regulation. EPA has consulted with DOT, and they...

  4. Tank characterization report for double shell tank 241-AP-104

    Energy Technology Data Exchange (ETDEWEB)

    Winkelman, W.D., Westinghouse Hanford

    1996-08-07

    This document summarizes the information on the historical uses, present status, and the sampling and analysis results of waste stored in Tank 241-AP-104. This report supports the requirements of Tri-Party Agreement Milestone M-44-09.

  5. A hot-spare injector for the APS linac.

    Energy Technology Data Exchange (ETDEWEB)

    Lewellen, J. W.

    1999-04-13

    Last year a second-generation SSRL-type thermionic cathode rf gun was installed in the Advanced Photon Source (APS) linac. This gun (referred to as ''gun2'') has been successfully commissioned and now serves as the main injector for the APS linac, essentially replacing the Koontz-type DC gun. To help ensure injector availability, particularly with the advent of top-up mode operation at the APS, a second thermionic-cathode rf gun will be installed in the APS linac to act as a hot-spare beam source. The hot-spare installation includes several unique design features, including a deep-orbit Panofsky-style alpha magnet. Details of the hot-spare beamline design and projected performance are presented, along with some plans for future performance upgrades.

  6. Lumbar pedicle screw placement: Using only AP plane imaging

    Directory of Open Access Journals (Sweden)

    Anil Sethi

    2012-01-01

    Conclusion: Placement of pedicle screws under fluoroscopic guidance using AP plane imaging alone with tactile guidance is safe, fast, and reliable. However, a good understanding of the radiographic landmarks is a prerequisite.

  7. Roles of AP-2 in clathrin-mediated endocytosis.

    Directory of Open Access Journals (Sweden)

    Emmanuel Boucrot

    Full Text Available BACKGROUND: The notion that AP-2 clathrin adaptor is an essential component of an endocytic clathrin coat appears to conflict with recent observations that substantial AP-2 depletion, using RNA interference with synthesis of AP-2 subunits, fails to block uptake of certain ligands known to internalize through a clathrin-based pathway. METHODOLOGY/PRINCIPAL FINDINGS: We report here the use of in vivo imaging data obtained by spinning-disk confocal microscopy to study the formation of clathrin-coated structures at the plasma membranes of BSC1 and HeLa cells depleted by RNAi of the clathrin adaptor, AP-2. Very few clathrin coats continue to assemble after AP-2 knockdown. Moreover, there is a total absence of clathrin-containing structures completely lacking AP-2 while all the remaining coats still contain a small amount of AP-2. These observations suggest that AP-2 is essential for endocytic coated-pit and coated-vesicle formation. We also find that AP-2 knockdown strongly inhibits light-density lipoprotein (LDL receptor-mediated endocytosis, as long as cells are maintained in complete serum and at 37 degrees C. If cells are first incubated with LDL at 4 degrees C, followed by warming, there is little or no decrease in LDL uptake with respect to control cells. LDL uptake at 37 degrees C is also not affected in AP-2 depleted cells first deprived of LDL by incubation with either serum-starved or LDL-starved cells for 24 hr. The LDL-deprived cells display a significant increase in endocytic structures enriched on deeply invaginated tubes that contain LDL and we suggest that under this condition of stress, LDL might enter through this alternative pathway. CONCLUSIONS/SIGNIFICANCE: These results suggest that AP-2 is essential for endocytic clathrin coated-pit and coated-vesicle formation. They also indicate that under normal conditions, functional endocytic clathrin coated pits are required for LDL internalization. We also show that under certain

  8. Army and Marine Corps Active Protection System (APS) Efforts

    Science.gov (United States)

    2016-08-30

    Summary Active Protection Systems (APSs) are subsystems integrated into or installed on a combat vehicle to automatically acquire, track, and respond...Protection System (APS) Efforts Congressional Research Service 1 Why This Issue Is Important for Congress Active Protection Systems (APSs) are...of these systems are highly susceptible to “soft kill” APS, which underlies the importance of both soft and hard kill capabilities. 20 In addition

  9. The Dushak–Erekdag Survey of roAp Stars

    Indian Academy of Sciences (India)

    Tatyana Dorokhova; Nikolay Dorokhov

    2005-06-01

    The search of roAp stars at Mt. Dushak–Erekdag Observatory was started in 1992 using the 0.8m Odessa telescope equipped with a two-star high-speed photometer. We have observed more than a dozen stars so far and discovered HD 99563 as roAp star while BD+8087 is suspected to have rapid oscillations. Negative results of our observations for the search of rapid oscillations in four stars in NGC 752 are also discussed.

  10. GeoDCAT-AP: Use cases and open issues

    OpenAIRE

    PEREGO ANDREA; FRIIS-CHRISTENSEN Anders; Lutz, Michael

    2016-01-01

    This paper illustrates some issues and use cases identified during the design and implementation of GeoDCAT-AP, a metadata profile aiming to provide a representation of geospatial metadata compliant with the DCAT application profile for European data portals (DCAT-AP). In particular, the paper focuses on those issues that may have a possible relevance also outside the geospatial domain, covering topics concerning metadata profile-based negotiation, publishing metadata on the Web, represen...

  11. AP-102/104 Retrieval control system qualification test procedure

    Energy Technology Data Exchange (ETDEWEB)

    RIECK, C.A.

    1999-05-18

    This Qualification Test Procedure documents the results of the qualification testing that was performed on the Project W-211, ''Initial Tank Retrieval Systems,'' retrieval control system (RCS) for tanks 241-AP-102 and 241-AP-104. The results confirm that the RCS has been programmed correctly and that the two related hardware enclosures have been assembled in accordance with the design documents.

  12. AP1000模块化施工分析%Analysis of the AP1000 Modular Construction

    Institute of Scientific and Technical Information of China (English)

    邹玮

    2014-01-01

    文章针对AP1000机组模块化施工的技术和进度特点进行了分析,特别是对AP1000模块化施工面临的挑战和问题进行了深入的分析.这些挑战和问题有些是首台AP1000机组所特有的,有些将在后续AP1000机组建设过程中继续存在,文章分别阐述并提出了几项建议措施.

  13. Data of evolutionary structure change: 1AP4A-1CLMA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1AP4A-1CLMA 1AP4 1CLM A A ---------------------------------------...DDS LTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLSLMARKMKEQDSEEELIEAFKVFDR...36 1CLM A 1CLMA

  14. APS DFD 2011 video submission V045

    CERN Document Server

    Swan, David; Ward, Thomas

    2011-01-01

    Inhomogeneous uid mixing in a tilted-rotating cylindrical tank (radius a = 3:5 cm) is shown at Re(17-40) and low capillary numbers. A water and surfactant solu- tion (1% by mass sodium oleate) is dispersed in soybean oil (95% by volume), through varying the rotation rate, and angle of inclination, the rate of mixing is observed. A planar laser is directed down the tank axis to highlight a cross-sectional area of the fluid volume and as the water droplets begin to break up to sizes on the order of the beam width and less, more light is refracted and the mixture is illuminated. Initially, the water breaks up into large droplets that exhibit approximate solid-body rotation about the bottom of the tank. When the total combined volume is below the critical volume of the tank Vcrit = a^3 tan vortex transport of the water occurs more rapidly, breaking up the water into continually smaller droplets in a process that resembles periodic shearing. When the fluid volume is above critical the water will break up and rotat...

  15. Authenticated, private, and secured smart cards (APS-SC)

    Science.gov (United States)

    Szu, Harold; Mehmood, Amir

    2006-04-01

    From historical perspective, the recent advancements in better antenna designs, low power circuitry integrations and inexpensive fabrication materials have made possible a miniature counter-measure against Radar, a clutter behaving like a fake target return called Digital Reflection Frequency Modulation (DRFM). Such a military counter-measure have found its way in the commerce as a near field communication known as Radio Frequency Identification (RFID), a passive or active item tag T attached to every readable-writable Smart Card (SC): Passports ID, medical patient ID, biometric ID, driver licenses, book ID, library ID, etc. These avalanche phenomena may be due to the 3 rd Gen phones seeking much more versatile & inexpensive interfaces, than the line-of-sight bar-code optical scan. Despite of the popularity of RFID, the lacking of Authenticity, Privacy and Security (APS) protection restricted somewhat the wide spread commercial, financial, medical, legal, and militarily applications. Conventional APS approach can obfuscate a private passkey K of SC with the tag number T or the reader number R, or both, i.e. only T*K or R*K or both will appear on them, where * denotes an invertible operation, e.g. EXOR, but not limited to it. Then, only the authentic owner, knowing all, can inverse the operation, e.g. EXOR*EXOR= I to find K. However, such an encryption could be easily compromised by a hacker seeking exhaustively by comparison based on those frequently used words. Nevertheless, knowing biological wetware lesson for power of pairs sensors and Radar hardware counter-measure history, we can counter the counter-measure DRFM, instead using one RFID tag per SD, we follow the Nature adopting two ears/tags, e.g. each one holding portions of the ID or simply two different ID's readable only by different modes of the interrogating reader, followed by brain central processor in terms of nonlinear invertible shufflers mixing two ID bits. We prefer to adopt such a hardware

  16. DNA cleavage at the AP site via β-elimination mediated by the AP site-binding ligands.

    Science.gov (United States)

    Abe, Yukiko S; Sasaki, Shigeki

    2016-02-15

    DNA is continuously damaged by endogenous and exogenous factors such as oxidation and alkylation. In the base excision repair pathway, the damaged nucleobases are removed by DNA N-glycosylase to form the abasic sites (AP sites). The alkylating antitumor agent exhibits cytotoxicity through the formation of the AP site. Therefore blockage or modulation of the AP site repair pathway may enhance the antitumor efficacy of DNA alkylating agents. In this study, we have examined the effects of the nucleobase-polyamine conjugated ligands (G-, A-, C- and T-ligands) on the cleavage of the AP site. The G- and A-ligands cleaved DNA at the AP site by promoting β-elimination in a non-selective manner by the G-ligand, and in a selective manner for the opposing dT by the A-ligand. These results suggest that the nucleobase-polyamine conjugate ligands may have the potential for enhancement of the cytotoxicities of the AP site.

  17. 49 CFR 192.371 - Service lines: Steel.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Service lines: Steel. 192.371 Section 192.371 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY... § 192.371 Service lines: Steel. Each steel service line to be operated at less than 100 p.s.i. (689...

  18. 49 CFR 192.377 - Service lines: Copper.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Service lines: Copper. 192.377 Section 192.377 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY... § 192.377 Service lines: Copper. Each copper service line installed within a building must be...

  19. Evaluation of transit lines plan

    OpenAIRE

    Cenek, Juraj

    2012-01-01

    The plan of mass transport or transit lines is a vital part of an organisation of public transportation services. An optimisation of the plan can improve the service either by reducing the operational costs or by improving of service quality. Three subjects interact in the problem namely state or local administration, transport companies and citizens. The plan optimisation should find the best compromise to satisfy interests of all actors. A relevant criterion must be defined to find the b...

  20. An AP4B1 frameshift mutation in siblings with intellectual disability and spastic tetraplegia further delineates the AP-4 deficiency syndrome.

    Science.gov (United States)

    Abdollahpour, Hengameh; Alawi, Malik; Kortüm, Fanny; Beckstette, Michael; Seemanova, Eva; Komárek, Vladimír; Rosenberger, Georg; Kutsche, Kerstin

    2015-02-01

    The recently proposed adaptor protein 4 (AP-4) deficiency syndrome comprises a group of congenital neurological disorders characterized by severe intellectual disability (ID), delayed or absent speech, hereditary spastic paraplegia, and growth retardation. AP-4 is a heterotetrameric protein complex with important functions in vesicle trafficking. Mutations in genes affecting different subunits of AP-4, including AP4B1, AP4E1, AP4S1, and AP4M1, have been reported in patients with the AP-4 deficiency phenotype. We describe two siblings from a non-consanguineous couple who presented with severe ID, absent speech, microcephaly, growth retardation, and progressive spastic tetraplegia. Whole-exome sequencing in the two patients identified the novel homozygous 2-bp deletion c.1160_1161delCA (p.(Thr387Argfs*30)) in AP4B1. Sanger sequencing confirmed the mutation in the siblings and revealed it in the heterozygous state in both parents. The AP4B1-associated phenotype has previously been assigned to spastic paraplegia-47. Identification of a novel AP4B1 alteration in two patients with clinical manifestations highly similar to other individuals with mutations affecting one of the four AP-4 subunits further supports the observation that loss of AP-4 assembly or functionality underlies the common clinical features in these patients and underscores the existence of the clinically recognizable AP-4 deficiency syndrome.

  1. AP1000灰棒燃料组件物理特性分析%Analysis of Physical Characteristics for AP 1000 Gray Rod Assembly

    Institute of Scientific and Technical Information of China (English)

    李晓静; 陈义学; 张斌; 张顺; 曹博; 余慧

    2013-01-01

    The AP1000 uses gray rods whose neutron absorption capability is lower than that of black rods to achieve load follow without requiring changes of the boron concen-tration .The use of gray rods results in reducing the amount of wastewater .Taking gray rod assembly in the AP1000 reactor as calculation model , transport-burnups were calculated by using the TRITON code of the SCALE5.1 code system .The results show that in the gray rod assemblies with different enrichments ,there is large difference in the eigenvalues at the beginning of fuel cycle between gray rod assemblies composed of different absorption materials .With the deepening of burnup ,the difference between them becomes smaller and the relative deviation is less than 1% ,the neutron absorption cross section of tungsten rods changes gentler relatively . T he results provide the reference for the actual operation of AP1000 reactor .%A P1000采用中子吸收能力低于黑棒的灰棒,在不改变冷却水中硼浓度的情况下完成反应堆中的负荷跟踪,从而减少了废水量。以AP1000压水堆中的灰棒燃料组件为计算模型,采用SCALE5.1程序系统中的TRITON模块对灰棒燃料组件进行输运燃耗计算。计算结果表明:在不同富集度的灰棒燃料组件中,两种不同材料组成的灰棒组件在最初燃耗的一段时间内特征值均相差较大,随着燃耗加深,其差别变小,相对偏差均在1%以内;钨棒的中子吸收截面相对变化较小。本工作的结果为A P1000反应堆的实际运行提供了参考。

  2. The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice.

    Directory of Open Access Journals (Sweden)

    Mahmoud W Yaish

    2010-09-01

    Full Text Available The interaction between phytohormones is an important mechanism which controls growth and developmental processes in plants. Deciphering these interactions is a crucial step in helping to develop crops with enhanced yield and resistance to environmental stresses. Controlling the expression level of OsAP2-39 which includes an APETALA 2 (AP2 domain leads to phenotypic changes in rice. Overexpression of OsAP2-39 leads to a reduction in yield by decreasing the biomass and the number of seeds in the transgenic rice lines. Global transcriptome analysis of the OsAP2-39 overexpression transgenic rice revealed the upregulation of a key abscisic acid (ABA biosynthetic gene OsNCED-I which codes for 9-cis-epoxycarotenoid dioxygenase and leads to an increase in the endogenous ABA level. In addition to OsNCED-1, the gene expression analysis revealed the upregulation of a gene that codes for the Elongation of Upper most Internode (EUI protein, an enzyme that catalyzes 16α, 17-epoxidation of non-13-hydroxylated GAs, which has been shown to deactivate gibberellins (GAs in rice. The exogenous application of GA restores the wild-type phenotype in the transgenic line and ABA application induces the expression of EUI and suppresses the expression of OsAP2-39 in the wild-type line. These observations clarify the antagonistic relationship between ABA and GA and illustrate a mechanism that leads to homeostasis of these hormones. In vivo and in vitro analysis showed that the expression of both OsNCED-1 and EUI are directly controlled by OsAP2-39. Together, these results reveal a novel mechanism for the control of the ABA/GA balance in rice which is regulated by OsAP2-39 that in turn regulates plant growth and seed production.

  3. Parthenolide inhibits ERK and AP-1 which are dysregulated and contribute to excessive IL-8 expression and secretion in cystic fibrosis cells

    Directory of Open Access Journals (Sweden)

    Saadane Aicha

    2011-10-01

    Full Text Available Abstract Background Excessive secretion of IL-8 characterizes cystic fibrosis (CF. This has been attributed to excessive activation of epithelial cell I-κB Kinase and/or NFκB. Maximum IL-8 production requires 3 cooperative mechanisms: 1 release of the promoter from repression; 2 activation of transcription by NFκB and AP-1; 3 stabilization of mRNA by p38-MAPK. Little is known about regulation of IL-8 by MAPKs or AP-1 in CF. Methods We studied our hypothesis in vitro using 3-cellular models. Two of these models are transformed cell lines with defective versus normal cystic fibrosis transmembrane conductance regulator (CFTR expression: an antisense/sense transfected cell line and the patient derived IB3-1/S9. In the third series of studies, we studied primary necropsy human tracheal epithelial cells treated with an inhibitor of CFTR function. All cell lines were pretreated with parthenolide and then stimulated with TNFα and/or IL-1β. Results In response to stimulation with TNFα and/or IL-1β, IL-8 production and mRNA expression was greater in CF-type cells than in non-CF controls. This was associated with enhanced phosphorylation of p38, ERK1/2 and JNK and increased activation of AP-1. Since we previously showed that parthenolide inhibits excessive IL-8 production by CF cells, we evaluated its effects on MAPK and AP-1 activation and showed that parthenolide inhibited ERK and AP-1 activation. Using a luciferase promoter assay, our studies showed that parthenolide decreased activation of the IL-8 promoter in CF cells stimulated with TNFα/IL-1β. Conclusions In addition to NFκB MAPKs ERK, JNK and p38 and the transcription factor AP-1 are also dysregulated in CF epithelial cells. Parthenolide inhibited both NFκB and MAPK/AP-1 pathways contributing to the inhibition of IL-8 production.

  4. Validation of the new trapped environment AE9/AP9/SPM at low Earth orbit

    Science.gov (United States)

    Badavi, Francis F.

    2014-09-01

    The completion of the international space station (ISS) in 2011 has provided the space research community an ideal proving ground for future long duration human activities in space. Ionizing radiation measurements in ISS form the ideal tool for the validation of radiation environmental models, nuclear transport codes and nuclear reaction cross sections. Indeed, prior measurements on the space transportation system (STS; shuttle) provided vital information impacting both the environmental models and the nuclear transport code developments by indicating the need for an improved dynamic model of the low Earth orbit (LEO) trapped environment. Additional studies using thermo-luminescent detector (TLD), tissue equivalent proportional counter (TEPC) area monitors, and computer aided design (CAD) model of earlier ISS configurations, confirmed STS observations that, as input, computational dosimetry requires an environmental model with dynamic and directional (anisotropic) behavior, as well as an accurate six degree of freedom (DOF) definition of the vehicle attitude and orientation along the orbit of ISS. At LEO, a vehicle encounters exposure from trapped particles and attenuated galactic cosmic rays (GCR). Within the trapped field, a challenge arises from properly estimating the amount of exposure acquired. There exist a number of models to define the intensities of the trapped particles during the solar quiet and active times. At active times, solar energetic particles (SEP) generated by solar flare or coronal mass ejection (CME) also contribute to the exposure at high northern and southern latitudes. Among the more established trapped models are the historic and popular AE8/AP8, dating back to the 1980s, the historic and less popular CRRES electron/proton, dating back to 1990s and the recently released AE9/AP9/SPM. The AE9/AP9/SPM model is a major improvement over the older AE8/AP8 and CRRES models. This model is derived from numerous measurements acquired over four

  5. Synthesis of an inositol hexakisphosphate (IP6) affinity probe to study the interactome from a colon cancer cell line.

    Science.gov (United States)

    Yin, Meng-Xin; Catimel, Bruno; Gregory, Mark; Condron, Melanie; Kapp, Eugene; Holmes, Andrew B; Burgess, Antony W

    2016-03-14

    Inositol hexakisphosphate (InsP6 or IP6) is an important signalling molecule in vesicular trafficking, neurotransmission, immune responses, regulation of protein kinases and phosphatases, activation of ion channels, antioxidant functions and anticancer activities. An IP6 probe was synthesised from myo-inositol via a derivatised analogue, which was immobilised through a terminal amino group onto Dynabeads. Systematic analysis of the IP6 interactome has been performed using the IP6 affinity probe using cytosolic extracts from the LIM1215 colonic carcinoma cell line. LC/MS/MS analysis identified 77 proteins or protein complexes that bind to IP6 specifically, including AP-2 complex proteins and β-arrestins as well as a number of novel potential IP6 interacting proteins. Bioinformatic enrichment analysis of the IP6 interactome reinforced the concept that IP6 regulates a number of biological processes including cell cycle and division, signal transduction, intracellular protein transport, vesicle-mediated transport and RNA splicing.

  6. Design of automatic transportation system of negative film production line based on master-slave PLC%基于PLC控制的负极片生产线自动输送系统的设计

    Institute of Scientific and Technical Information of China (English)

    孙贵政; 杨建国; 王庆霞

    2012-01-01

    负极片是高性能能量供给部件之一,负极片生产又是空间电源制造的关键环节.通过对负极片生产线的工作原理和工艺流程的分析,设计了以主-从站PLC和触摸屏为控制核心的负极片生产线自动输送系统.上位机以触摸屏为载体,设计了手动操作及自动运行的界面,下位机的软件系统设计了手动工作方式和自动工作方式的控制系统.该输送系统用四个电机驱动的机械手、两条自动输送线和一个银网放置转盘实现了自动放纸、放模、放网、脱模等物料输送的自动化,有效地提高了负极片生产的效率,避免了有毒物质对生产人员健康的损害.%Negative film is kind of high-performance power supply component negative film production is the key step of space power manufacturing.Through the negative film production line working principle and process analysis , designed a negative film production line automated delivery system based on master-slave PLC and touching screen,achieved materials automatic transportation including paper,mold,silver nets,etc.Effectively improve the efficiency of the negative film production and avoid the toxic substances damaging the health of production personnel.

  7. Target and peripheral dose during patient repositioning with the Gamma Knife automatic positioning system (APS) device.

    Science.gov (United States)

    Tran, Tuan-Anh; Stanley, Thomas R; Malhotra, Harish K; De Boer, Steven F; Prasad, Dheerendra; Podgorsak, Matthew B

    2010-01-28

    The GammaPlan treatment planning system does not account for the leakage and scatter dose during APS repositioning. In this study, the dose delivered to the target site and its periphery from the defocus stage and intershot couch transit (couch motion from the focus to defocus position and back) associated with APS repositioning are measured for the Gamma Knife model 4C. A stereotactic head-frame was attached to a Leksell 16 cm diameter spherical phantom with a calibrated ion chamber at its center. Using a fiducial box, CT images of the phantom were acquired and registered in the GammaPlan treatment planning system to determine the coordinates of the target (center of the phantom). An absorbed dose of 10 Gy to the 50% isodose line was prescribed to the target site for all measurements. Plans were generated for the 8, 14 and 18 mm collimator helmets to determine the relationship of measured dose to the number of repositions of the APS system and to the helmet size. The target coordinate was identical throughout entire study and there was no movement of the APS between various shots. This allowed for measurement of intershot transit dose at the target site and its periphery. The couch was paused in the defocus position, allowing defocus dose measurements at the intracranial target and periphery. Measured dose increases with frequency of repositioning and with helmet collimator size. During couch transit, the target receives more dose than peripheral regions; however, in the defocus position, the greatest dose is superior to the target site. The automatic positioning system for the Leksell Gamma Knife model 4C results in an additional dose of up to 3.87 +/- 0.07%, 4.97 +/- 0.04%, and 5.71 +/- 0.07% to the target site; its periphery receives additional dose that varies depending on its position relative to the target. There is also dose contribution to the patient in the defocus position, where the APS repositions the patient from one treatment coordinate to another

  8. Exploring Mars: The Ares Payload Service (APS)

    Science.gov (United States)

    Bowen, Justin; Lusignan, Bruce

    1999-08-01

    design notably an improved re-entry cooling system and fueling stability analysis were done this year. More technical detail and a proposed international consortium to develop the SSTO is presented in another session of this year's Mars convention. We believe that there will be no human exploration of Mars based on the Shuttle or Expendable launch vehicles, and no resources available except for a cooperative international program. However, just as the world is learning to cooperate in peacekeeping, we hold out the hope that similar cooperation will develop for Mars exploration. With that in mind, this year we asked the question- "How will the human mission get to Mars if it has to use the SsTO for transportation?"

  9. Control of machine functions or transport systems

    Energy Technology Data Exchange (ETDEWEB)

    Woodley, M.D.; Lee, M.J.; Jaeger, J.; King, A.S.

    1983-01-01

    A computer code, COMFORT, has been developed at SLAC for on-line calculation of the strengths of magnetic elements in an electron storage ring or transport beam line, subject to first order fitting constraints on the ring or beam line parameters. This code can also be used off-line as an interactive lattice or beam line design tool.

  10. Prevention of aspiration pneumonia (AP) with oral care.

    Science.gov (United States)

    Tada, Akio; Miura, Hiroko

    2012-01-01

    AP is a major cause of morbidity and mortality in elderly patients, especially frail elderly patients. The aim of this article is to review effect of oral care, including oral hygiene and improvement of oral function, on the prevention of AP among elderly people in hospitals and nursing homes. There is now a substantial body of work studying the effect of oral care on the prevention of respiratory diseases. Oral hygiene, consisting of oral decontamination and mechanical cleaning by dental professionals, has resulted in significant clinical effects (decreased incidence of pneumonia and decreased mortality from respiratory diseases) in clinical randomized trials. Moreover, studies examining oral colonization by pneumonia pathogens have shown the effect of oral hygiene on eliminating these pathogens. In addition, swallowing training has been shown to improve the movement and function of swallowing-related muscles, also resulting in decreased incidence of pneumonia. These findings support the contention that oral care is effective in the prevention of AP.

  11. Westinghouse plans global new builds for AP1000

    Energy Technology Data Exchange (ETDEWEB)

    Mitev, Lubomir [NucNet, Brussels (Belgium)

    2014-10-15

    Interview with Danny Roderick, Westinghouse Electric Company, President and Chief Executive Officer since September 2012, about perspectives and future plans for AP1000 new build worldwide. Within three to four years there wille be 'shovels in the ground' for three new AP1000 reactors in the UK, as well as new units in China and Bulgaria. Four AP1000 reactors are under construction in the United States at Vogtle and VC Summer, and soon at Turkey Point. Additionally Danny Roderick spoke about the acquisition of NuGen, technology transfer, the influence of the Ukraine crises on the nuclear market in East Europe and the future need for more nuclear worldwide and in the UK and Bulgaria.

  12. Quercetin modulates NF-kappa B and AP-1/JNK pathways to induce cell death in human hepatoma cells.

    Science.gov (United States)

    Granado-Serrano, Ana Belén; Martín, María Angeles; Bravo, Laura; Goya, Luis; Ramos, Sonia

    2010-01-01

    Quercetin, a dietary flavonoid, has been shown to possess anticarcinogenic properties, but the precise molecular mechanisms of action are not thoroughly elucidated. The aim of this study was to investigate the regulatory effect of quercetin (50 microM) on two main transcription factors (NF-kappa B and AP-1) related to survival/proliferation pathways in a human hepatoma cell line (HepG2) over time. Quercetin induced a significant time-dependent inactivation of the NF-kappa B pathway consistent with a downregulation of the NF-kappa B binding activity (from 15 min onward). These features were in concert with a time-dependent activation (starting at 15 min and maintained up to 18 h) of the AP-1/JNK pathway, which played an important role in the control of the cell death induced by the flavonoid and contributed to the regulation of survival/proliferation (AKT, ERK) and death (caspase-3, p38, unbalance of Bcl-2 proapoptotic and antiapoptotic proteins) signals. These data suggest that NF-kappa B and AP-1 play a main role in the tight regulation of survival/proliferation pathways exerted by quercetin and that the sustained JNK/AP-1 activation and inhibition of NF-kappa B provoked by the flavonoid induced HepG2 death.

  13. First Magnetic Doppler Images of a roAp star

    Science.gov (United States)

    Lüftinger, T.; Kochukhov, O.; Ryabchikova, T.; Weiss, W. W.; Ilyin, I.

    2007-06-01

    We present the first analysis of the magnetic field geometry and elemental abundance distributions on the surface of a rapidly oscillating Ap (roAp) star, using an elaborate magnetic Doppler Imaging (MDI) code (Piskunov et al. 2002, Kochukhov et al. 2002), inv, which allows to reconstruct simultaneously and consistently the magnetic field geometry and abundance distributions on a stellar surface without any a priori assumptions. We analysed Stokes I and V time series obtained with the SOFIN polarimeter and recovered the magnetic field and surface abundance structures of Fe and Nd (among others). These two elements are found to be anticorrelated.

  14. THE PARALLEL RECURSIVE AP ADAPTIVE ALGORITHM BASED ON VOLTERRA SERIES

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Aiming at the nonlinear system identification problem, a parallel recursive affine projection (AP) adaptive algorithm for the nonlinear system based on Volterra series is presented in this paper. The algorithm identifies in parallel the Volterra kernel of each order, recursively estimate the inverse of the autocorrelation matrix for the Volterra input of each order, and remarkably improve the convergence speed of the identification process compared with the NLMS and conventional AP adaptive algorithm based on Volterra series. Simulation results indicate that the proposed method in this paper is efficient.

  15. Calibration of Strömgren-Crawford photometry for Ap-stars compared to Hipparcos results

    Science.gov (United States)

    Vogt, Nikolaus; Paunzen, Ernst; Maitzen, Hans M.

    structure in the distribution around the 45-line. Only in the case of normal late type stars one does note that part of the stars have obviously fainter photometric absolute magnitudes as compared to their Hipparcos based values. Turning now to our Ap-sample - bearing in mind that assigning the proper calibration group is not always trivial - we do in fact notice systematics: The photometric calibration for the early group stars yields too bright absolute magnitudes which may be related to the well known fact that Bp stars have too small Balmer jumps as well as Balmer lines. Statistically, this effect would lead to an overestimate of the distances by about 25 percent. The situation is reversed for the intermediate and late group of Ap stars: the lower the temperature, the fainter becomes the photometrically derived absolute magnitude in comparison to the value calculated from the Hipparcos parallaxes. While in the case of intermediate Ap-stars the apparent luminosity difference only rarely surpasses one magnitude, the late type peculiars are photometrically underluminous by roughly 1.5 magnitudes on the average. The outstanding systematic behaviour of the late type Ap-stars is reminiscent of the nonsymmetric distribution of normal late A-type stars (mentioned before) and could be interpreted by the presence of non-detected peculiar stars in the sample of normal stars. In the light of these findings the Strömgren-Crawford calibrations will be approximately reliable only if applied to CP2 stars at the transition from the early to the intermediate group. Endeavours should concentrate on appropriate peculiarity corrections for stars outside this domain.

  16. Integrating Particulate Representations into AP Chemistry and Introductory Chemistry Courses

    Science.gov (United States)

    Prilliman, Stephen G.

    2014-01-01

    The College Board's recently revised curriculum for advanced placement (AP) chemistry places a strong emphasis on conceptual understanding, including representations of particle phenomena. This change in emphasis is informed by years of research showing that students could perform algorithmic calculations but not explain those calculations…

  17. Penetration dynamics of AP8 in thin ceramic tiles

    NARCIS (Netherlands)

    Abadjieva, E.; Khoe, Y.S.

    2013-01-01

    The interaction of thin ceramic tiles with AP8 (WC core, 7,62 mm) at 1000 m/s velocity has been studied experimentally and numerically. “Thin” ceramic tiles refers here to ratio of the tile thickness (t) to the projectile diameter, (d), t/d@ 1, as they are both in the same order. The method applied

  18. Research on CoAP%CoAP协议研究

    Institute of Scientific and Technical Information of China (English)

    刘紫青; 程燕; 关联; 桑梓勤; 郝俊瑞

    2013-01-01

    For some M2M applications, there exists problems of constrained networks and nodes, and the HTTP isn't applicable. The Constrained Application Protocol (CoAP) is described and analyzed in this paper, and the mapping between CoAP and HTTP is also included. Then, the comparison of the two protocols is researched. Finally, it can be concluded that CoAP is simple and its overhead is very low, which can well meet the application layer' s communications requirements in the constrained environment.%针对某些M2M应用中存在网络和节点资源受限的情况,不适用HTTP,介绍和分析了受限应用协议CoAP(Constrained Application Protocol)的特点及其与HTTP的映射,并将CoAP和HTTP进行对比研究,发现CoAP简单、开销小,能很好地满足资源受限环境的应用层通信需求.

  19. RELATIVISTIC SUPERNOVAE HAVE SHORTER-LIVED CENTRAL ENGINES OR MORE EXTENDED PROGENITORS: THE CASE OF SN 2012ap

    Energy Technology Data Exchange (ETDEWEB)

    Margutti, R.; Milisavljevic, D.; Soderberg, A. M.; Sanders, N.; Chakraborti, S.; Kamble, A.; Drout, M.; Parrent, J.; Zauderer, A. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Guidorzi, C. [Department of Physics and Earth Sciences, University of Ferrara, via Saragat 1, I-44122 Ferrara (Italy); Morsony, B. J. [Department of Astronomy, University of Wisconsin-Madison, 2535 Sterling Hall, 475 North Charter Street, Madison, WI 53706-1582 (United States); Ray, A. [Department of Astronomy and Astrophysics, Tata Institute of Fundamental Research, 1 Homi Bhabha Road, Mumbai 400 005 (India); Chomiuk, L. [Department of Physics and Astronomy, Michigan State University, East Lansing, MI 48824 (United States)

    2014-12-20

    Deep, late-time X-ray observations of the relativistic, engine-driven, type Ic SN 2012ap allow us to probe the nearby environment of the explosion and reveal the unique properties of relativistic supernova explosions (SNe). We find that on a local scale of ∼0.01 pc the environment was shaped directly by the evolution of the progenitor star with a pre-explosion mass-loss rate of M-dot <5×10{sup −6} M{sub ⊙} yr{sup −1}, in line with gamma-ray bursts (GRBs) and the other relativistic SN 2009bb. Like sub-energetic GRBs, SN 2012ap is characterized by a bright radio emission and evidence for mildly relativistic ejecta. However, its late-time (δt ≈ 20 days) X-ray emission is ∼100 times fainter than the faintest sub-energetic GRB at the same epoch, with no evidence for late-time central engine activity. These results support theoretical proposals that link relativistic SNe like 2009bb and 2012ap with the weakest observed engine-driven explosions, where the jet barely fails to break out. Furthermore, our observations demonstrate that the difference between relativistic SNe and sub-energetic GRBs is intrinsic and not due to line-of-sight effects. This phenomenology can either be due to an intrinsically shorter-lived engine or to a more extended progenitor in relativistic SNe.

  20. Cinnamon extract induces tumor cell death through inhibition of NFκB and AP1

    Directory of Open Access Journals (Sweden)

    Lee Sung

    2010-07-01

    Full Text Available Abstract Background Cinnamomum cassia bark is the outer skin of an evergreen tall tree belonging to the family Lauraceae containing several active components such as essential oils (cinnamic aldehyde and cinnamyl aldehyde, tannin, mucus and carbohydrate. They have various biological functions including anti-oxidant, anti-microbial, anti-inflammation, anti-diabetic and anti-tumor activity. Previously, we have reported that anti-cancer effect of cinnamon extracts is associated with modulation of angiogenesis and effector function of CD8+ T cells. In this study, we further identified that anti-tumor effect of cinnamon extracts is also link with enhanced pro-apoptotic activity by inhibiting the activities NFκB and AP1 in mouse melanoma model. Methods Water soluble cinnamon extract was obtained and quality of cinnamon extract was evaluated by HPLC (High Performance Liquid Chromatography analysis. In this study, we tested anti-tumor activity and elucidated action mechanism of cinnamon extract using various types of tumor cell lines including lymphoma, melanoma, cervix cancer and colorectal cancer in vitro and in vivo mouse melanoma model. Results Cinnamon extract strongly inhibited tumor cell proliferation in vitro and induced active cell death of tumor cells by up-regulating pro-apoptotic molecules while inhibiting NFκB and AP1 activity and their target genes such as Bcl-2, BcL-xL and survivin. Oral administration of cinnamon extract in melanoma transplantation model significantly inhibited tumor growth with the same mechanism of action observed in vitro. Conclusion Our study suggests that anti-tumor effect of cinnamon extracts is directly linked with enhanced pro-apoptotic activity and inhibition of NFκB and AP1 activities and their target genes in vitro and in vivo mouse melanoma model. Hence, further elucidation of active components of cinnamon extract could lead to development of potent anti-tumor agent or complementary and alternative

  1. 100% N2 atmospheric-pressure microwave-line-plasma production with a modified waveguide structure

    Science.gov (United States)

    Suzuki, Haruka; Tamura, Yuto; Itoh, Hitoshi; Sekine, Makoto; Hori, Masaru; Toyoda, Hirotaka

    2016-09-01

    Large-scale atmospheric pressure (AP) plasmas have been given much attention because of its high cost benefit and a variety of possibilities for industrial applications. Microwave discharge plasma using slot antenna is attractive due to its ability of high-density and stable plasma production, and we have developed a long-scale AP microwave plasma (AP microwave line plasma: AP-MLP) source using loop-structured waveguide and travelling wave and have reported spatially-uniform AP-MLP of 40 cm in length using Ar or He gas discharge. However, rare gas discharge is not always suitable for industrial applications because usage of large volume rare gas degrades the AP cost benefit. Furthermore, many industrial applications require chemically-reactive species and the AP-MLP using molecular gas will drastically increase the applications of the AP-MLP. In this study, we demonstrate 100% N2 discharge of the AP-MLP with a modified waveguide structure. Cross-sectional structure of the waveguide is improved to enhance the microwave electric field in the slot. 100% N2 plasma of 15 cm-long is successfully produced using CW microwave power of 2 kW. Low gas temperature of 1000 K is confirmed by optical emission spectroscopy, suggesting applications of the AP-MLP to low temperature processes. Part of this work is supported by JSPS KAKENHI Grant Number 25286079.

  2. High Line

    DEFF Research Database (Denmark)

    Kiib, Hans

    2015-01-01

    At just over 10 meters above street level, the High Line extends three kilometers through three districts of Southwestern Manhattan in New York. It consists of simple steel construction, and previously served as an elevated rail line connection between Penn Station on 34th Street and the many....... The High Line project has been carried out as part of an open conversion strategy. The result is a remarkable urban architectural project, which works as a catalyst for the urban development of Western Manhattan. The greater project includes the restoration and reuse of many old industrial buildings...... in close proximity to the park bridge and new projects being added to fit the context. The outcome is a conglomeration of non-contemporary urban activities along the High Line, where mechanical workshops, small wholesale stores. etc. mix with new exclusive residential buildings, eminent cafés...

  3. High Line

    DEFF Research Database (Denmark)

    Kiib, Hans

    2015-01-01

    in close proximity to the park bridge and new projects being added to fit the context. The outcome is a conglomeration of non-contemporary urban activities along the High Line, where mechanical workshops, small wholesale stores. etc. mix with new exclusive residential buildings, eminent cafés......At just over 10 meters above street level, the High Line extends three kilometers through three districts of Southwestern Manhattan in New York. It consists of simple steel construction, and previously served as an elevated rail line connection between Penn Station on 34th Street and the many...... factories and warehouses on Gansevoort Street. Today the High Line is a beautiful park covered with new tiles, viewing platforms and smaller recreational areas. The park bridge has simple, uniform, urban fittings and features a variety of flowering plants, grasses, shrubs and trees from around the world...

  4. Differential Roles of Grb2 and AP-2 in p38 MAPK- and EGF-Induced EGFR Internalization

    DEFF Research Database (Denmark)

    Grandal, Michael V; Grøvdal, Lene M; Henriksen, Lasse;

    2012-01-01

    also can induce EGFR endocytosis. This endocytosis lacks many of the characteristics of ligand-induced EGFR endocytosis. We compared the two types of endocytosis with regard to the requirements for proteins in the internalization machinery. Both types of endocytosis require clathrin, but while...... epidermal growth factor (EGF)-induced EGFR internalization also required Grb2, p38 MAPK-induced internalization did not. Interestingly, AP-2 knock down blocked p38 MAPK-induced EGFR internalization, but only mildly affected EGF-induced internalization. In line with this, simultaneously mutating two AP-2...... interaction sites in EGFR affected p38 MAPK-induced internalization much more than EGF-induced EGFR internalization. Thus, it seems that EGFR in the two situations uses different sets of internalization mechanisms....

  5. Alternating group covers of the affine line

    CERN Document Server

    Muskat, Jeremy

    2009-01-01

    We prove Abhyankar's Inertia Conjecture for the alternating group A_{p+2} on p+2 letters when p = 2 mod 3, by showing that every possible inertia group occurs for a (wildly ramified) A_{p+2}-Galois cover of the projective k-line branched only at infinity where k is an algebraically closed field of characteristic p > 0. More generally, when 1 < s < p and gcd(p-1, s+1)=1, we prove that all but finitely many rational numbers which satisfy the obvious necessary conditions occur as the upper jump in the filtration of higher ramification groups of an A_{p+s}-Galois cover of the projective line branched only at infinity.

  6. KIC 7582608: a new Kepler roAp star with frequency variability

    Directory of Open Access Journals (Sweden)

    Holdsworth D.L.

    2015-01-01

    Full Text Available We analyse the fifth roAp star reported in the Kepler field, KIC 7582608, discovered with the SuperWASP project. The object shows a high frequency pulsation at 181.7324d−1 (P = 7.9 min with an amplitude of 1.45 mmag, and low frequency rotational modulation corresponding to a period of 20.4339 d. Spectral analysis confirms the Ap nature of the target, with characteristic lines of rare earth elements present. From our spectral observations we derive a lower limit on the mean magnetic field modulus of 〈B〉 =3.05 ± 0.23 kG. Long Cadence Kepler observations show a frequency quintuplet split by the rotational period of the star, typical for an oblique pulsator. We suggest the star is a quadrupole pulsator with a geometry such that i ~ 66° and β ~ 33°. We detect frequency variations of the pulsation in both the WASP and Kepler data sets on many time scales. Linear, non-adiabatic stability modelling allows us to constrain a region on the HR diagram where the pulsations are unstable, an area consistent with observations.

  7. Analysis of N-16 concentration in primary cooling system of AP1000 power reactor

    Energy Technology Data Exchange (ETDEWEB)

    Rohanda, Anis [Center for Reactor Technology and Nuclear Safety – BATAN Kawasan PUSPIPTEK Gd. No. 80 Serpong, Tangerang Selatan 15310 (Indonesia); Waris, Abdul [Physics Department of ITB, Indonesia anis-rohanda@yahoo.com (Indonesia)

    2015-04-16

    Nitrogen-16 (N-16) is one of the radiation safety parameter on the primary reactor system. The activation product, N-16, is the predominant contributor to the activity in the reactor coolant system during reactor operation. N-16 is activation product derived from activation of O-16 with fast neutron based on {sup 16}O(n,p){sup 16}N reaction. Thus study is needed and it performs to determine N-16 concentration in reactor coolant (primary coolant) in supporting radiation safety. One of the way is using analytical methode based on activation and redecay princip to obtain N-16 concentration. The analysis was performed on the configuration basis and operational of Westinghouse AP1000 power reactor in several monitoring points at coolant reactor system. The results of the calculation of N-16 concentration at the core outlet, reactor vessel outlet, pressurizer line, inlet and outlet of steam generators, primary pumps, reactor vessels inlet and core inlet are: 281, 257, 255, 250, 145, 142, 129 and 112 µCi/gram respectively. The results of analysis compared with AP1000 design control document as standard values. The verification showed very high accuracy comparation between analytical results and standard values.

  8. Orbital parameters, chemical composition, and magnetic field of the Ap binary HD 98088

    CERN Document Server

    Folsom, C P; Wade, G A; Kochukhov, O; Alecian, E; Shulyak, D

    2013-01-01

    HD 98088 is a synchronised, double-lined spectroscopic binary system with a magnetic Ap primary component and an Am secondary component. We study this rare system using high-resolution MuSiCoS spectropolarimetric data, to gain insight into the effect of binarity on the origin of stellar magnetism and the formation of chemical peculiarities in A-type stars. Using a new collection of 29 high-resolution Stokes VQU spectra we re-derive the orbital and stellar physical parameters and conduct the first disentangling of spectroscopic observations of the system to conduct spectral analysis of the individual stellar components. From this analysis we determine the projected rotational velocities of the stars and conduct a detailed chemical abundance analysis of each component using both the SYNTH3 and ZEEMAN spectrum synthesis codes. The surface abundances of the primary component are typical of a cool Ap star, while those of the secondary component are typical of an Am star. We present the first magnetic analysis of b...

  9. 77 FR 14979 - Transportation Conformity Rule Restructuring Amendments

    Science.gov (United States)

    2012-03-14

    ... AGENCY 40 CFR Part 93 RIN 2060-AP57 Transportation Conformity Rule Restructuring Amendments AGENCY... conformity rule to finalize provisions that were proposed on August 13, 2010. These amendments restructure several sections of the transportation conformity rule so that they apply to any new or revised...

  10. Heparin (GAG-hed inhibits LCR activity of Human Papillomavirus type 18 by decreasing AP1 binding

    Directory of Open Access Journals (Sweden)

    López-Bayghen Esther

    2006-08-01

    Full Text Available Abstract Background High risk HPVs are causative agents of anogenital cancers. Viral E6 and E7 genes are continuously expressed and are largely responsible for the oncogenic activity of these viruses. Transcription of the E6 and E7 genes is controlled by the viral Long Control Region (LCR, plus several cellular transcription factors including AP1 and the viral protein E2. Within the LCR, the binding and activity of the transcription factor AP1 represents a key regulatory event in maintaining E6/E7 gene expression and uncontrolled cell proliferation. Glycosaminoglycans (GAGs, such as heparin, can inhibit tumour growth; they have also shown antiviral effects and inhibition of AP1 transcriptional activity. The purpose of this study was to test the heparinoid GAG-hed, as a possible antiviral and antitumoral agent in an HPV18 positive HeLa cell line. Methods Using in vivo and in vitro approaches we tested GAG-hed effects on HeLa tumour cell growth, cell proliferation and on the expression of HPV18 E6/E7 oncogenes. GAG-hed effects on AP1 binding to HPV18-LCR-DNA were tested by EMSA. Results We were able to record the antitumoral effect of GAG-hed in vivo by using as a model tumours induced by injection of HeLa cells into athymic female mice. The antiviral effect of GAG-hed resulted in the inhibition of LCR activity and, consequently, the inhibition of E6 and E7 transcription. A specific diminishing of cell proliferation rates was observed in HeLa but not in HPV-free colorectal adenocarcinoma cells. Treated HeLa cells did not undergo apoptosis but the percentage of cells in G2/M phase of the cell cycle was increased. We also detected that GAG-hed prevents the binding of the transcription factor AP1 to the LCR. Conclusion Direct interaction of GAG-hed with the components of the AP1 complex and subsequent interference with its ability to correctly bind specific sites within the viral LCR may contribute to the inhibition of E6/E7 transcription and cell

  11. AP24534, a Pan-BCR-ABL Inhibitor for Chronic Myeloid Leukemia, Potently Inhibits the T315I Mutant and Overcomes Mutation-Based Resistance

    Energy Technology Data Exchange (ETDEWEB)

    O’Hare, Thomas; Shakespeare, William C.; Zhu, Xiaotian; Eide, Christopher A.; Rivera, Victor M.; Wang, Frank; Adrian, Lauren T.; Zhou, Tianjun; Huang, Wei-Sheng; Xu, Qihong; Metcalf, III, Chester A.; Tyner, Jeffrey W.; Loriaux, Marc M.; Corbin, Amie S.; Wardwell, Scott; Ning, Yaoyu; Keats, Jeffrey A.; Wang, Yihan; Sundaramoorthi, Raji; Thomas, Mathew; Zhou, Dong; Snodgrass, Joseph; Commodore, Lois; Sawyer, Tomi K.; Dalgarno, David C.; Deininger, Michael W.N.; Druker, Brian J.; Clackson, Tim; (OHSU- Cancer Instit.); (ARIAD)

    2010-09-07

    Inhibition of BCR-ABL by imatinib induces durable responses in many patients with chronic myeloid leukemia (CML), but resistance attributable to kinase domain mutations can lead to relapse and a switch to second-line therapy with nilotinib or dasatinib. Despite three approved therapeutic options, the cross-resistant BCR-ABL{sup T315I} mutation and compound mutants selected on sequential inhibitor therapy remain major clinical challenges. We report design and preclinical evaluation of AP24534, a potent, orally available multitargeted kinase inhibitor active against T315I and other BCR-ABL mutants. AP24534 inhibited all tested BCR-ABL mutants in cellular and biochemical assays, suppressed BCR-ABL{sup T315I}-driven tumor growth in mice, and completely abrogated resistance in cell-based mutagenesis screens. Our work supports clinical evaluation of AP24534 as a pan-BCR-ABL inhibitor for treatment of CML.

  12. Modelling the incomplete Paschen-Back effect in the spectra of magnetic Ap stars

    Science.gov (United States)

    Stift, M. J.; Leone, F.; Landi Degl'Innocenti, E.

    2008-04-01

    We present preliminary results of a systematic investigation of the incomplete Paschen-Back effect in magnetic Ap stars. A short overview of the theory is followed by a demonstration of how level splittings and component strengths change with magnetic field strength for some lines of special astrophysical interest. Requirements are set out for a code which allows the calculation of full Stokes spectra in the Paschen-Back regime, and the behaviour of Stokes I and V profiles of transitions in the multiplet 74 of FeII is discussed in some detail. It is shown that the incomplete Paschen-Back effect can lead to notable line shifts which strongly depend on total multiplet strength, magnetic field strength and field direction. Ghost components (which violate the normal selection rule on J) show up in strong magnetic fields but are probably unobservable. Finally it is shown that measurements of the integrated magnetic field modulus Hs are not adversely affected by the Paschen-Back effect, and that there is a potential problem in (magnetic) Doppler mapping if lines in the Paschen-Back regime are treated in the Zeeman approximation.

  13. Diffusion and Settling in Ap/Bp Stars

    Energy Technology Data Exchange (ETDEWEB)

    Turcotte, S

    2003-04-09

    Ap/Bp stars are magnetic chemically peculiar early A and late B type stars of the main sequence. They exhibit peculiar surface abundance anomalies that are thought to be the result of gravitational settling and radiative levitation. The physics of diffusion in these stars are reviewed briefly and some model predictions are discussed. While models reproduce some observations reasonably well, more work is needed before the behavior of diffusing elements in a complex magnetic field is fully understood.

  14. On the progenitor of the Type Ic supernova 2002ap

    NARCIS (Netherlands)

    Smartt, SJ; Ramirez-Ruiz, E; Gilmore, GF; Meikle, WPS; Ferguson, AMN; Knapen, JH

    2002-01-01

    This Letter presents wide-field optical and near-IR (UBVRIHalphaK') images of the galaxy M74 that were taken between 0.6 and 8.3 yr before the discovery of the Type Ic supernova 2002ap. We have located the position of the supernova on these images with an accuracy of 0."3. We find no sign of a proge

  15. A Low-Power CoAP for Contiki

    OpenAIRE

    Kovatsch M.; Duquennoy S.; Dunkels A.

    2011-01-01

    Internet of Things devices will by and large be battery-operated, but existing application protocols have typically not been designed with power-efficiency in mind. In low-power wireless systems, power-efficiency is determined by the ability to maintain a low radio duty cycle: keeping the radio off as much as possible. We present an implementation of the IETF Constrained Application Protocol (CoAP) for the Contiki operating system that leverages the ContikiMAC low-powe...

  16. Characteristics of shigellosis outbreaks in the AP of Vojvodina

    OpenAIRE

    2009-01-01

    Introduction. Shigellosis causes around 165 million infections and around 1 million deaths in the world every year. Two thirds of both infections and deaths are among children younger than 10. Shigellosis mainly spreads by direct or indirect contact, but water- and food-borne outbreaks are not rare. Material and methods. A descriptive epidemiological method was used to analyse characteristics of shigellosis outbreaks in the AP of Vojvodina in the period 1979-2005. Results. During this period ...

  17. Diffusion and Settling in Ap/Bp Stars

    CERN Document Server

    Turcotte, S

    2003-01-01

    Ap/Bp stars are magnetic chemically peculiar early A and late B type stars of the main sequence. They exhibit peculiar surface abundance anomalies that are thought to be the result of gravitational settling and radiative levitation. The physics of diffusion in these stars are reviewed briefly and some model predictions are discussed. While models reproduce some observations reasonably well, more work is needed before the behavior of diffusing elements in a complex magnetic field is fully understood.

  18. Essay: Computers in the APS Editorial Office: The Early Years

    Science.gov (United States)

    Adams, Peter

    2009-03-01

    APS journals in the late 1960s began to move from hot-metal print shops to tailor-made typewriters, next to special keyboards and a small computer, then to completely photocomposed journal pages. At the same time, internal editorial operations were being converted from paper handling and data records to completely electronic procedures. By 1990, it became feasible for authors to submit manuscripts to the journal electronically.

  19. Heterologous expression of ApGSMT2 and ApDMT2 genes from Aphanothece halophytica enhanced drought tolerance in transgenic tobacco.

    Science.gov (United States)

    He, Ying; He, Chunmei; Li, Lihua; Liu, Zhili; Yang, Aifang; Zhang, Juren

    2011-01-01

    The glycine-methylation biosynthetic pathway of glycinebetaine (GB) has been investigated, but only a few studies on GB accumulation in transgenic higher plants have utilized this pathway. In this study, two methyltransferase genes named ApGSMT2 and ApDMT2, encoding proteins catalyzing GB biosynthesis from glycine, were cloned from a relative strain of Aphanothece halophytica. The potential roles of ApGSMT2 and ApDMT2 in GB synthesis were first examined in transgenic Escherichia coli, which had increased levels of GB and improved salt tolerance. Then ApGSMT2 and ApDMT2 were transferred into tobacco. Compared with transgenic tobacco expressing betA, transgenic tobacco co-expressing ApGSMT2 and ApDMT2 accumulated more GB and exhibited enhanced drought resistance with better germination performance, higher relative water content, less cell membrane damage and better photosynthetic capacity under drought stress. We concluded that the ApGSMT2 and ApDMT2 genes cloned in this study will be very useful for engineering GB-accumulating transgenic plants with enhanced drought resistance.

  20. Long-term stability of the APS storage ring

    Energy Technology Data Exchange (ETDEWEB)

    Friedsam, H.; Penicka, M.; Error, J.

    1999-10-26

    The Advanced Photon Source (APS), a third-generation synchrotrons light source, was commissioned in 1995 at Argonne ''National Laboratory and has been fully-operational for beamline users since 1997. The APS storage ring can accommodate up to 68 user beamlines; about 70% of the available beamlines are currently in use by various collaborative access teams (CATS). The 7-GeV synchrotrons light source produces light in the soft to hard x-ray range that is used for research in such areas as x-ray instrumentation; material, chemical and atomic sciences; biology; and geo/soil/ environmental sciences. For the successful operation of an x-ray light source such as the Advanced Photon Source, the long-term stability of the concrete floor supporting the beam components and user beamlines is crucial. Settlements impact the orbit and location of the x-ray source points as well as the position of the x-ray beamlines. This paper compares the results of two successive resurveys of the APS accelerator components performed in 1995 and 1998.

  1. AP Theory IV: Intrinsic Topological Quantum Langlands Theory

    CERN Document Server

    Winkelnkemper, H E

    2010-01-01

    Without using any moduli, sheaves, stacks, nor any analytic, nor category-type arguments, we exhibit an analogue to Geometric Langlands Theory in an entirely model-independent, non-perturbative,purely smooth topological context in Artin Presentation Theory. A basic initial feature is that AP Theory, as a whole, is already, ab initio, a universal canonical 2D sigma-model, targeting smooth, compact, simply-connected 4-manifolds with a connected boundary, and its topological Planckian quantum starting point, as well as its cone-like, infinitely-generated at each stage, graded group of homology-preserving, but topology-changing transitions/interactions, exhibit the most general qualitative S-duality. We first point out the numerous mathematically rigorous, model-free, (i.e., intrinsic), topological AP analogues with the heuristic Kapustin-Witten version of Geometric Langlands theory, as well as the crucial differences between the two theories. The latter have to exist since AP Theory deals, a priori, essentially ...

  2. 49 CFR 192.719 - Transmission lines: Testing of repairs.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Transmission lines: Testing of repairs. 192.719... TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Maintenance § 192.719 Transmission lines: Testing of repairs. (a) Testing of replacement pipe. If a segment of transmission line...

  3. 49 CFR 192.745 - Valve maintenance: Transmission lines.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Valve maintenance: Transmission lines. 192.745... TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Maintenance § 192.745 Valve maintenance: Transmission lines. (a) Each transmission line valve that might be required during any...

  4. Doppler imaging of chemical spots on magnetic Ap/Bp stars. Numerical tests and assessment of systematic errors

    CERN Document Server

    Kochukhov, O

    2016-01-01

    Doppler imaging (DI) is a powerful spectroscopic inversion technique that enables conversion of a line profile time series into a two-dimensional map of the stellar surface inhomogeneities. In this paper we investigate the accuracy of chemical abundance DI of Ap/Bp stars and assess the impact of several different systematic errors on the reconstructed spot maps. We simulate spectroscopic observational data for different spot distributions in the presence of a moderately strong dipolar magnetic field. We then reconstruct chemical maps using different sets of spectral lines and making different assumptions about line formation in the inversion calculations. Our numerical experiments demonstrate that a modern DI code successfully recovers the input chemical spot distributions comprised of multiple circular spots at different latitudes or an element overabundance belt at the magnetic equator. For the optimal reconstruction the average reconstruction errors do not exceed ~0.10 dex. The errors increase to about 0.1...

  5. Radiation Transport

    Energy Technology Data Exchange (ETDEWEB)

    Urbatsch, Todd James [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-06-15

    We present an overview of radiation transport, covering terminology, blackbody raditation, opacities, Boltzmann transport theory, approximations to the transport equation. Next we introduce several transport methods. We present a section on Caseology, observing transport boundary layers. We briefly broach topics of software development, including verification and validation, and we close with a section on high energy-density experiments that highlight and support radiation transport.

  6. The appearance of beam lines

    Energy Technology Data Exchange (ETDEWEB)

    Carey, D.C.

    1993-05-01

    The combination of an existing graphics package with a large program like TRANSPORT has often resulted in considerable modification to the large program. Use of other graphics package has resulted in essentially having to repeat the work. This difficulty has been avoided in a modification of TRANSPORT which produce layouts of beam lines. Drawings of the reference trajectory and three-dimensional images of all magnets are made by the graphics package TOP DRAWER. Nothing specific to TOP DRAWER or any other graphics has been incorporated into TRANSPORT. If a user is with a different graphics package he or she can then begin usage of this alternate package essentially immediately.

  7. The Adolescent Novel in AP English: A Response to Patricia Spencer.

    Science.gov (United States)

    McGee, Tim

    1992-01-01

    Discusses the absence of adolescent literature in Advanced Placement (AP) English classes. Describes how a class of AP students and their teacher benefited from reading Robert Cormier's "The Chocolate War." (PRA)

  8. Recombinant thermostable AP exonuclease from Thermoanaerobacter tengcongensis: cloning, expression, purification, properties and PCR application

    DEFF Research Database (Denmark)

    Dabrowski, Slawomir; Brillowska-Dabrowska, Anna; Ahring, Birgitte Kiær

    2013-01-01

    Apurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. The gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis...... (14000 kU) of pure His6-tagged Tte AP (153 kU/mg) from 1 liter of culture. The optimal conditions of Tte AP endo-, exonuclease and 3'-nuclease activity were investigated using fluorescein labeled dsDNA with inserted AP sites and ssDNA. Optimal Tte AP endonuclease activity was observed at 70-75 degrees C...... DNA templates. Tte AP exonuclease eliminated AP-sites in DNA template and improved the efficiency of DNA amplification....

  9. NRC confirmatory AP600 safety system phase I testing in the ROSA/AP600 test facility

    Energy Technology Data Exchange (ETDEWEB)

    Rhee, G.S.; Kukita, Yutaka; Schultz, R.R.

    1996-03-01

    The NRC confirmatory phase I testing for the AP600 safety systems has been completed in the modified ROSA (Rig of Safety Assessment) test facility located at the Japan Atomic Energy Research Institute (JAERI) campus in Tokai, Japan. The test matrix included a variety of accident scenarios covering both design and beyond-design basis accidents. The test results indicate the AP600 safety systems as reflected in ROSA appear to perform as designed and there is no danger of core heatup for the accident scenarios investigated. In addition, no detrimental system interactions nor adverse effects of non-safety systems on the safety system functions were identified. However, three phenomena of interest have been identified for further examination to determine whether they are relevant to the AP600 plant. Those three phenomena are: (1) a potential for water hammer caused by rapid condensation which may occur following the actuation of the automatic depressurization system (ADS), (2) a large thermal gradient in the cold leg pipe where cooled water returns from the passive residual heat removal system and forms a thermally stratified layer, and (3) system-wide oscillations initiating following the ADS stage 4 actuation and persisting until the liquid in the pressurizer drains and steam generation in the core becomes insignificant.

  10. Comparative sensitivity of three mosquito cell lines for isolation of dengue viruses.

    Science.gov (United States)

    Kuno, G; Gubler, D J; Vélez, M; Oliver, A

    1985-01-01

    Comparative studies were carried out on three mosquito cell lines (C6/36 clone of Aedes albopictus, AP-61 from A. pseudoscutellaris, and TRA-284 from Toxorhynchites amboinensis) to determine their sensitivity to dengue virus isolation, growth, and handling characteristics for immunofluorescent testing. Virus isolation rates from human sera were the highest in the TRA-284-SF (a line adapted to serum-free medium), followed by the TRA-284 parental line and AP-61. Virus isolation was the lowest in the C6/36 line. All 3 cell lines were comparable in terms of ease of handling, but C6/36 cells were preferable for detecting infected cells by the direct fluorescent antibody test (DFAT) because of frequent cell clumping in the AP-61 and TRA-284 lines. Early detection of viral antigen of all 4 serotypes in the infected cells by DFAT was dependent upon the virus titre in the serum. The AP-61 and TRA-284-SF cells were the best for early detection and identification of viral antigen. Similarly, both AP-61 and TRA-284 cells were more resistant than C6/36 cells to toxic effects of human sera. Based on the economy of using the serum-free medium, their higher sensitivity for dengue virus isolation, and their ease of handling, it is recommended that the TRA-284-SF cell line be used for routine dengue virus isolation in laboratories with cell culture capability.

  11. Comparative sensitivity of three mosquito cell lines for isolation of dengue viruses*

    Science.gov (United States)

    Kuno, G.; Gubler, D. J.; Vélez, M.; Oliver, A.

    1985-01-01

    Comparative studies were carried out on three mosquito cell lines (C6/36 clone of Aedes albopictus, AP-61 from A. pseudoscutellaris, and TRA-284 from Toxorhynchites amboinensis) to determine their sensitivity to dengue virus isolation, growth, and handling characteristics for immunofluorescent testing. Virus isolation rates from human sera were the highest in the TRA-284-SF (a line adapted to serum-free medium), followed by the TRA-284 parental line and AP-61. Virus isolation was the lowest in the C6/36 line. All 3 cell lines were comparable in terms of ease of handling, but C6/36 cells were preferable for detecting infected cells by the direct fluorescent antibody test (DFAT) because of frequent cell clumping in the AP-61 and TRA-284 lines. Early detection of viral antigen of all 4 serotypes in the infected cells by DFAT was dependent upon the virus titre in the serum. The AP-61 and TRA-284-SF cells were the best for early detection and identification of viral antigen. Similarly, both AP-61 and TRA-284 cells were more resistant than C6/36 cells to toxic effects of human sera. Based on the economy of using the serum-free medium, their higher sensitivity for dengue virus isolation, and their ease of handling, it is recommended that the TRA-284-SF cell line be used for routine dengue virus isolation in laboratories with cell culture capability. PMID:2861916

  12. Chamber transport

    Energy Technology Data Exchange (ETDEWEB)

    OLSON,CRAIG L.

    2000-05-17

    Heavy ion beam transport through the containment chamber plays a crucial role in all heavy ion fusion (HIF) scenarios. Here, several parameters are used to characterize the operating space for HIF beams; transport modes are assessed in relation to evolving target/accelerator requirements; results of recent relevant experiments and simulations of HIF transport are summarized; and relevant instabilities are reviewed. All transport options still exist, including (1) vacuum ballistic transport, (2) neutralized ballistic transport, and (3) channel-like transport. Presently, the European HIF program favors vacuum ballistic transport, while the US HIF program favors neutralized ballistic transport with channel-like transport as an alternate approach. Further transport research is needed to clearly guide selection of the most attractive, integrated HIF system.

  13. FRANX. Application for analysis and quantification of the APS fire; FRANK. Aplicacion para el analisis y cuantificacion de los APS de incendios

    Energy Technology Data Exchange (ETDEWEB)

    Snchez, A.; Osorio, F.; Ontoso, N.

    2014-07-01

    The FRANX application has been developed by EPRI within the Risk and Reliability User Group in order to facilitate the process of quantification and updating APS Fire (also covers floods and earthquakes). By applying fire scenarios are quantified in the central integrating the tasks performed during the APS fire. This paper describes the main features of the program to allow quantification of an APS Fire. (Author)

  14. AP Studio Art as an Enabling Constraint for Secondary Art Education

    Science.gov (United States)

    Graham, Mark A.

    2009-01-01

    Advanced Placement (AP) Studio Art is an influential force in secondary art education as is evident in the 31,800 portfolios submitted for review in 2008. From the perspectives of a high school educator and AP Reader, this author has observed how the constraints of the AP program can be used to generate support for high school art programs and…

  15. Using a Classroom Response System to Improve Multiple-Choice Performance in AP[R] Physics

    Science.gov (United States)

    Bertrand, Peggy

    2009-01-01

    Participation in rigorous high school courses such as Advanced Placement (AP[R]) Physics increases the likelihood of college success, especially for students who are traditionally underserved. Tackling difficult multiple-choice exams should be part of any AP program because well-constructed multiple-choice questions, such as those on AP exams and…

  16. Differentiation of zebrafish melanophores depends on transcription factors AP2 alpha and AP2 epsilon.

    Directory of Open Access Journals (Sweden)

    Eric Van Otterloo

    2010-09-01

    Full Text Available A model of the gene-regulatory-network (GRN, governing growth, survival, and differentiation of melanocytes, has emerged from studies of mouse coat color mutants and melanoma cell lines. In this model, Transcription Factor Activator Protein 2 alpha (TFAP2A contributes to melanocyte development by activating expression of the gene encoding the receptor tyrosine kinase Kit. Next, ligand-bound Kit stimulates a pathway activating transcription factor Microphthalmia (Mitf, which promotes differentiation and survival of melanocytes by activating expression of Tyrosinase family members, Bcl2, and other genes. The model predicts that in both Tfap2a and Kit null mutants there will be a phenotype of reduced melanocytes and that, because Tfap2a acts upstream of Kit, this phenotype will be more severe, or at least as severe as, in Tfap2a null mutants in comparison to Kit null mutants. Unexpectedly, this is not the case in zebrafish or mouse. Because many Tfap2 family members have identical DNA-binding specificity, we reasoned that another Tfap2 family member may work redundantly with Tfap2a in promoting Kit expression. We report that tfap2e is expressed in melanoblasts and melanophores in zebrafish embryos and that its orthologue, TFAP2E, is expressed in human melanocytes. We provide evidence that Tfap2e functions redundantly with Tfap2a to maintain kita expression in zebrafish embryonic melanophores. Further, we show that, in contrast to in kita mutants where embryonic melanophores appear to differentiate normally, in tfap2a/e doubly-deficient embryonic melanophores are small and under-melanized, although they retain expression of mitfa. Interestingly, forcing expression of mitfa in tfap2a/e doubly-deficient embryos partially restores melanophore differentiation. These findings reveal that Tfap2 activity, mediated redundantly by Tfap2a and Tfap2e, promotes melanophore differentiation in parallel with Mitf by an effector other than Kit. This work

  17. Structural characterization of the N-terminal mineral modification domains from the molluscan crystal-modulating biomineralization proteins, AP7 and AP24.

    Science.gov (United States)

    Wustman, Brandon A; Morse, Daniel E; Evans, John Spencer

    2004-08-05

    The AP7 and AP24 proteins represent a class of mineral-interaction polypeptides that are found in the aragonite-containing nacre layer of mollusk shell (H. rufescens). These proteins have been shown to preferentially interfere with calcium carbonate mineral growth in vitro. It is believed that both proteins play an important role in aragonite polymorph selection in the mollusk shell. Previously, we demonstrated the 1-30 amino acid (AA) N-terminal sequences of AP7 and AP24 represent mineral interaction/modification domains in both proteins, as evidenced by their ability to frustrate calcium carbonate crystal growth at step edge regions. In this present report, using free N-terminal, C(alpha)-amide "capped" synthetic polypeptides representing the 1-30 AA regions of AP7 (AP7-1 polypeptide) and AP24 (AP24-1 polypeptide) and NMR spectroscopy, we confirm that both N-terminal sequences possess putative Ca (II) interaction polyanionic sequence regions (2 x -DD- in AP7-1, -DDDED- in AP24-1) that are random coil-like in structure. However, with regard to the remaining sequences regions, each polypeptide features unique structural differences. AP7-1 possesses an extended beta-strand or polyproline type II-like structure within the A11-M10, S12-V13, and S28-I27 sequence regions, with the remaining sequence regions adopting a random-coil-like structure, a trait common to other polyelectrolyte mineral-associated polypeptide sequences. Conversely, AP24-1 possesses random coil-like structure within A1-S9 and Q14-N16 sequence regions, and evidence for turn-like, bend, or loop conformation within the G10-N13, Q17-N24, and M29-F30 sequence regions, similar to the structures identified within the putative elastomeric proteins Lustrin A and sea urchin spicule matrix proteins. The similarities and differences in AP7 and AP24 N-terminal domain structure are discussed with regard to joint AP7-AP24 protein modification of calcium carbonate growth.

  18. DELTA : a computer code for determination of efficiency of particulate matter and aerosol transport; DELTA : un codice di calcolo per la determinazione dell`efficienza delle linee di prelievo e trasporto dell`aerosol

    Energy Technology Data Exchange (ETDEWEB)

    Picini, P.; Caropreso, G.; Antonini, A.; Galuppi, G.; Sbrana, M. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dip. Ambiente; Bardone, G. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dip. Energia; Malvestuto, V.; Ricotta, A. [CNR, Rome (Italy). Ist. di Fisica dell`Atmosfera

    1996-04-01

    In the Part I of this paper a mathematical model to calculate the sampling and transport efficiencies (both in laminar and turbulent condition) of any sampling and transport system decomposable in several cylindrical elemental component is presented. In the Part II an experimental facility built in Casaccia ENEA laboratory is described and the measures carried out to validate the model are reported.

  19. Operation of the APS photoinjector drive laser system.

    Energy Technology Data Exchange (ETDEWEB)

    Li, Y.; Accelerator Systems Division (APS)

    2008-08-04

    The APS photoinjector drive laser system has been in operation since 1999 and is achieving a performance level exceeding the requirement of stable operation of the LEUTL FEL system. One remarkable number is the UV energy stability of better than 2% rms, sometimes less than 1% rms. This report summarizes the operation experience of the laser system and the improvements made along the way. We also outline the route of upgrade of the system and some frontier laser research and development opportunities in ultrabright electron beam generation.

  20. Desarrollo y Estudio del protocolo Observe para CoAP

    OpenAIRE

    Gimeno Gimenez, Javier

    2013-01-01

    Premi Accenture al millor Projecte Final de Carrera, Treball Fi de Grau, Master Thesis, en l'Àrea de Serveis Telemàtics (curs 2012-2013) [ANGLÈS] In the context of the wireless sensor networks, an implementation of the observer pattern for CoAP is done. The implementation is done both for the nodes of the network and for a proxy that will allow to establish object-observer relationships between sensors inside the WSN and elements outside the network. [CASTELLÀ] En el contexto de las red...

  1. Ethernet ring protection with managed FDB using APS payload

    Science.gov (United States)

    Im, Jinsung; Ryoo, Jeong-dong; Joo, Bheom Soon; Rhee, J.-K. Kevin

    2007-11-01

    Ethernet ring protection (ERP) is a new technology based on OAM (operations, administration, and maintenance) being standardized by the ITU-T G.8032 working group. In this paper, we present the recent development of Ethernet ring protection which is called FDB (filtering database) flush scheme and propose a new Ethernet ring protection technique introducing a managed FDB using APS to deliver information how to fix FDB selectively. We discuss the current development of the ERP technology at ITU-T and performance comparisons between different proposals.

  2. AP/二茂铁衍生物混合体系的热感度研究%Study on thermal sensitivity of AP and ferrocene derivative mixtures

    Institute of Scientific and Technical Information of China (English)

    王宁; 赵孝彬; 罗岚; 李彦荣; 段卫毅

    2011-01-01

    通过局部热感度试验和DSC-TG联用仪,研究了高氯酸铵(AP)和一种二茂铁衍生物(EMT)混合体系的热感度.结果表明,减小AP粒度或增加EMT含量,能缩小AP高低温分解峰之间的差距,提高AP低温分解量,混合物的局部热感度增加.AP存在下,EMT的侧链容易发生断裂,断裂后的二茂铁或二茂铁离子活性体对AP分解起催化作用.此外,EMT 不利于AP的质子转移过程,但对HClO4的分解有较强催化作用.%Thermal sensitivity of AP and ferrocene derivative (EMT) mixtures was studied by partial thermal sensitivity and DSC-TG coupling. The results indicate that reducing AP particle size or increasing the content of EMT can shorten the gap between high-temperature thermal decomposition peak of AP and low-temperature thermal decomposition peak of AP, enhance the low-temperature thermad decomposition amount of AP,and increase partial thermal sensitivity about mixtures of AP and EMT. In the presence of AP, side-chain of EMT is easily ruptured. Residual main skeleton, ferrocene or ferrocene ion, is the real catalyst to thermal decomposition of AP. Furthermore, EMT inhabits proton transfer process of AP, but it is a strong catalysis of EMT to decomposition of HCIO4, one of initial decomposition product of AP.

  3. NF-κB/AP-1-Targeted Inhibition of Macrophage-Mediated Inflammatory Responses by Depigmenting Compound AP736 Derived from Natural 1,3-Diphenylpropane Skeleton

    Directory of Open Access Journals (Sweden)

    Van Thai Ha

    2014-01-01

    Full Text Available AP736 was identified as an antimelanogenic drug that can be used for the prevention of melasma, freckles, and dark spots in skin by acting as a suppressor of melanin synthesis and tyrosinase expression. Since macrophage-mediated inflammatory responses are critical for skin health, here we investigated the potential anti-inflammatory activity of AP736. The effects of AP736 on various inflammatory events such as nitric oxide (NO/prostaglandin (PG E2 production, inflammatory gene expression, phagocytic uptake, and morphological changes were examined in RAW264.7 cells. AP736 was found to strongly inhibit the production of both NO and PGE2 in lipopolysaccharide- (LPS- treated RAW264.7 cells. In addition, AP736 strongly inhibited both LPS-induced morphological changes and FITC-dextran-induced phagocytic uptake. Furthermore, AP736 also downregulated the expression of multiple inflammatory genes, such as inducible NO synthase (iNOS, cyclooxygenase- (COX- 2, and interleukin- (IL- 1β in LPS-treated RAW264.7 cells. Transcription factor analysis, including upstream signalling events, revealed that both NF-κB and AP-1 were targeted by AP736 via inhibition of the IKK/IκBα and IRAK1/TAK1 pathways. Therefore, our results strongly suggest that AP736 is a potential anti-inflammatory drug due to its suppression of NF-κB-IKK/IκBα and AP-1-IRAK1/TAK1 signalling, which may make AP736 useful for the treatment of macrophage-mediated skin inflammation.

  4. Modelling the incomplete Paschen-Back effect in the spectra of magnetic Ap stars

    CERN Document Server

    Stift, M J; Degl'Innocenti, E Landi

    2008-01-01

    We present first results of a systematic investigation of the incomplete Paschen-Back effect in magnetic Ap stars. A short overview of the theory is followed by a demonstration of how level splittings and component strengths change with magnetic field strength for some lines of special astrophysical interest. Requirements are set out for a code which allows the calculation of full Stokes spectra in the Paschen-Back regime and the behaviour of Stokes I and V profiles of transitions in the multiplet 74 of FeII is discussed in some detail. It is shown that the incomplete Paschen-Back effect can lead to noticeable line shifts which strongly depend on total multiplet strength, magnetic field strength and field direction. Ghost components (which violate the normal selection rule on J) show up in strong magnetic fields but are probably unobservable. Finally it is shown that measurements of the integrated magnetic field modulus $H_s$ are not adversely affected by the Paschen-Back effect, and that there is a potential...

  5. KIC 4768731: a bright long-period roAp star in the Kepler Field

    CERN Document Server

    Smalley, B; Murphy, S J; Lehmann, H; Kurtz, D W; Holdsworth, D L; Cunha, M S; Balona, L A; Briquet, M; Bruntt, H; de Cat, P; Lampens, P; Thygesen, A O; Uytterhoeven, K

    2015-01-01

    We report the identification of 61.45 d^-1 (711.2 mu Hz) oscillations, with amplitudes of 62.6-mu mag, in KIC 4768731 (HD 225914) using Kepler photometry. This relatively bright (V=9.17) chemically peculiar star with spectral type A5 Vp SrCr(Eu) has previously been found to exhibit rotational modulation with a period of 5.21 d. Fourier analysis reveals a simple dipole pulsator with an amplitude that has remained stable over a 4-yr time span, but with a frequency that is variable. Analysis of high-resolution spectra yields stellar parameters of T_eff = 8100 +/- 200 K, log g = 4.0 +/- 0.2, [Fe/H] = +0.31 +/- 0.24 and v sin i = 14.8 +/- 1.6 km/s. Line profile variations caused by rotation are also evident. Lines of Sr, Cr, Eu, Mg and Si are strongest when the star is brightest, while Y and Ba vary in anti-phase with the other elements. The abundances of rare earth elements are only modestly enhanced compared to other roAp stars of similar T_eff and log g. Radial velocities in the literature suggest a significant...

  6. production lines

    Directory of Open Access Journals (Sweden)

    Jingshan Li

    2000-01-01

    Full Text Available In this work, serial production lines with finished goods buffers operating in the pull regime are considered. The machines are assumed to obey Bernoulli reliability model. The problem of satisfying customers demand is addressed. The level of demand satisfaction is quantified by the due-time performance (DTP, which is defined as the probability to ship to the customer a required number of parts during a fixed time interval. Within this scenario, the definitions of DTP bottlenecks are introduced and a method for their identification is developed.

  7. Molecular Basis for Enhancement of the Meiotic DMCI Recombinase by RAD51AP1

    Energy Technology Data Exchange (ETDEWEB)

    Dray, Eloise; Dunlop, Myun Hwa; Kauppi, Liisa; San Filippo, Joseph San; Wiese, Claudia; Tsai, Miaw-Sheue; Begovic, Sead; Schild, David; Jasin, Maria; Keeney, Scott; Sung, Patrick

    2010-11-05

    Homologous recombination is needed for meiotic chromosome segregation, genome maintenance, and tumor suppression. RAD51AP1 (RAD51 Associated Protein 1) has been shown to interact with and enhance the recombinase activity of RAD51. Accordingly, genetic ablation of RAD51AP1 leads to enhanced sensitivity to and also chromosome aberrations upon DNA damage, demonstrating a role for RAD51AP1 in mitotic homologous recombination. Here we show physical association of RAD51AP1 with the meiosis-specific recombinase DMC1 and a stimulatory effect of RAD51AP1 on the DMC1-mediated D-loop reaction. Mechanistic studies have revealed that RAD51AP1 enhances the ability of the DMC1 presynaptic filament to capture the duplex DNA partner and to assemble the synaptic complex, in which the recombining DNA strands are homologously aligned. We also provide evidence that functional co-operation is dependent on complex formation between DMC1 and RAD51AP1, and that distinct epitopes in RAD51AP1 mediate interactions with RAD51 and DMC1. Finally, we show that RAD51AP1 is expressed in mouse testes, and that RAD51AP1 foci co-localize with a subset of DMC1 foci in spermatocytes. These results suggest that RAD51AP1 also serves an important role in meiotic homologous recombination.

  8. 49 CFR 192.365 - Service lines: Location of valves.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Service lines: Location of valves. 192.365 Section..., and Service Lines § 192.365 Service lines: Location of valves. (a) Relation to regulator or meter. Each service-line valve must be installed upstream of the regulator or, if there is no...

  9. 49 CFR 192.706 - Transmission lines: Leakage surveys.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Transmission lines: Leakage surveys. 192.706... Transmission lines: Leakage surveys. Leakage surveys of a transmission line must be conducted at intervals not exceeding 15 months, but at least once each calendar year. However, in the case of a transmission line...

  10. Data Aggregation Gateway Framework for CoAP Group Communications

    Directory of Open Access Journals (Sweden)

    Minki Cha

    2016-11-01

    Full Text Available In this paper, a data aggregation gateway framework (DA-GW for constrained application protocol (CoAP group communications is proposed. The DA-GW framework is designed to improve the throughput performance and energy efficiency of group communication to monitor and control multiple sensor devices collectively with a single user terminal. The DA-GW consists of four function blocks—the message analyzer, group manager, message scheduler and data handler—and three informative databases—the client database, resource database and information database. The DA-GW performs group management and group communication through each functional block and stores resources in the informative databases. The DA-GW employs international standard-based data structures and provides the interoperability of heterogeneous devices used in various applications. The DA-GW is implemented using a Java-based open source framework called jCoAP to evaluate the functions and performance of the DA-GW. The experiment results showed that the DA-GW framework revealed better performance than existing group communication methods in terms of throughput and energy consumption.

  11. Pulsation models for the roAp star HD 134214

    CERN Document Server

    Saio, H; Weiss, W W; Matthews, J M; Ryabchikova, T

    2011-01-01

    Precise time-series photometry with the MOST satellite has led to identification of 10 pulsation frequencies in the rapidly oscillating Ap (roAp) star HD 134214. We have fitted the observed frequencies with theoretical frequencies of axisymmetric modes in a grid of stellar models with dipole magnetic fields. We find that, among models with a standard composition of $(X,Z) = (0.70,0.02)$ and with suppressed convection, eigenfrequencies of a $1.65\\,{\\rm M}_\\odot$ model with $\\log T_{\\rm eff} = 3.858$ and a polar magnetic field strength of 4.1kG agree best with the observed frequencies. We identify the observed pulsation frequency with the largest amplitude as a deformed dipole ($\\ell = 1$) mode, and the four next-largest-amplitude frequencies as deformed $\\ell = 2$ modes. These modes have a radial quasi-node in the outermost atmospheric layers ($\\tau \\sim 10^{-3}$). Although the model frequencies agree roughly with observed ones, they are all above the acoustic cut-off frequency for the model atmosphere and hen...

  12. The new AP Physics exams: Integrating qualitative and quantitative reasoning

    Science.gov (United States)

    Elby, Andrew

    2015-04-01

    When physics instructors and education researchers emphasize the importance of integrating qualitative and quantitative reasoning in problem solving, they usually mean using those types of reasoning serially and separately: first students should analyze the physical situation qualitatively/conceptually to figure out the relevant equations, then they should process those equations quantitatively to generate a solution, and finally they should use qualitative reasoning to check that answer for plausibility (Heller, Keith, & Anderson, 1992). The new AP Physics 1 and 2 exams will, of course, reward this approach to problem solving. But one kind of free response question will demand and reward a further integration of qualitative and quantitative reasoning, namely mathematical modeling and sense-making--inventing new equations to capture a physical situation and focusing on proportionalities, inverse proportionalities, and other functional relations to infer what the equation ``says'' about the physical world. In this talk, I discuss examples of these qualitative-quantitative translation questions, highlighting how they differ from both standard quantitative and standard qualitative questions. I then discuss the kinds of modeling activities that can help AP and college students develop these skills and habits of mind.

  13. A Fe K Line in GRB 970508

    Science.gov (United States)

    Protassov, R.; van Dyk, D.; Connors, A.; Kashyap, V.; Siemiginowska, A.

    2000-12-01

    We examine the x-ray spectrum of the afterglow of GRB 970508, analyzed for Fe line emission by Piro et al (1999, ApJL, 514, L73). This is a difficult and extremely important measurement: the detection of x-ray afterglows from γ -ray bursts is at best a tricky business, relying on near-real satellite time response to unpredictable events; and a great deal of luck in catching a burst bright enough for a useful spectral analysis. Detecting a clear atomic (or cyclotron) line in the generally smooth and featureless afterglow (or burst) emission not only gives one of the few very specific keys to the physics local to the emission region, but also provides clues or confirmation of its distance (via redshift). Unfortunately, neither the likelihood ratio test or the related F-statistic commonly used to detect spectral lines adhere to their nominal Chi square and F-distributions. Thus we begin by calibrating the F-statistic used in Piro et al (1999, ApJL, 514, L73) via a simulation study. The simulation study relies on a completely specified source model, i.e. we do Monte Carlo simulations with all model parameters fixed (so--called ``parametric bootstrapping''). Second, we employ the method of posterior predictive p-values to calibrate a LRT statistic while accounting for the uncertainty in the parameters of the source model. Our analysis reveals evidence for the Fe K line.

  14. RAD51AP1-deficiency in vertebrate cells impairs DNA replication.

    Science.gov (United States)

    Parplys, Ann C; Kratz, Katja; Speed, Michael C; Leung, Stanley G; Schild, David; Wiese, Claudia

    2014-12-01

    RAD51-associated protein 1 (RAD51AP1) is critical for homologous recombination (HR) by interacting with and stimulating the activities of the RAD51 and DMC1 recombinases. In human somatic cells, knockdown of RAD51AP1 results in increased sensitivity to DNA damaging agents and to impaired HR, but the formation of DNA damage-induced RAD51 foci is unaffected. Here, we generated a genetic model system, based on chicken DT40 cells, to assess the phenotype of fully inactivated RAD51AP1 in vertebrate cells. Targeted inactivation of both RAD51AP1 alleles has no effect on either viability or doubling-time in undamaged cells, but leads to increased levels of cytotoxicity after exposure to cisplatin or to ionizing radiation. Interestingly, ectopic expression of GgRAD51AP1, but not of HsRAD51AP1 is able to fully complement in cell survival assays. Notably, in RAD51AP1-deficient DT40 cells the resolution of DNA damage-induced RAD51 foci is greatly slowed down, while their formation is not impaired. We also identify, for the first time, an important role for RAD51AP1 in counteracting both spontaneous and DNA damage-induced replication stress. In human and in chicken cells, RAD51AP1 is required to maintain wild type speed of replication fork progression, and both RAD51AP1-depleted human cells and RAD51AP1-deficient DT40 cells respond to replication stress by a slow-down of replication fork elongation rates. However, increased firing of replication origins occurs in RAD51AP1-/- DT40 cells, likely to ensure the timely duplication of the entire genome. Taken together, our results may explain why RAD51AP1 commonly is overexpressed in tumor cells and tissues, and we speculate that the disruption of RAD51AP1 function could be a promising approach in targeted tumor therapy.

  15. 储氢合金/AP/HTPB推进剂燃烧性能研究%Investigation on Combustion Properties of Hydrogen Storage Alloy/AP/HTPB Propellant

    Institute of Scientific and Technical Information of China (English)

    窦燕蒙; 罗运军; 李国平; 葛震

    2013-01-01

    AI/AP/HTPB propellant and hydrogen storage alloy/AP/HTPB propellant were prepared and their properties were characterized by DSC,PDSC,explosion heat and burning rate test. DSC and PDSC test results show that hydrogen storage alloy has catalysis on condensed phase reactions of AP/HTPB propellant in which main decomposition temperature is decreased by 21.67℃,and 15. 67 ℃ at 0. 1MPa and 5MPa,respectively while condensed phase reaction heat is increased by 105. 97% and 21. 87% separately. Explosion heat test results show that hydrogen storage alloy has catalysis on gas phase reactions of AP/HTPB propellant in which gas phase reaction heat is increased by 4. 74% . Burning rate test results show that hydrogen storage alloy can increase burning rate of AP/HTPB propellant. Further studies indicate that possible correlation exist between burning rate and explosion heat of Al/AP/HTPB propellant and hydrogen storage alloy/AP/HTPB propellant. The burning rate-explosion heat formula is summed up.%制备了Al/AP/HTPB推进剂和储氢合金/AP/HTPB推进剂,并对它们进行了DSC,PDSC,爆热和燃速测试.DSC和PDSC测试结果表明,储氢合金对AP/HTPB推进剂的凝聚相反应有催化作用,在0.1MPa和5MPa下,使其凝聚相主分解温度分别降低21.67℃和15.67℃,凝聚相反应热分别提高105.97%和21.87%;爆热测试结果表明,储氢合金对AP/HTPB推进剂的气相反应有催化作用,使气相反应热提高4.74%;燃速结果表明,储氢合金可以提高AP/HTPB推进剂的燃速.进一步研究表明,A1/AP/HTPB推进剂和储氢合金/AP/HTPB推进剂的燃速与爆热存在一定的相关性.总结出了燃速-爆热关系式.

  16. Expansion and Functional Divergence of AP2 Group Genes in Spermatophytes Determined by Molecular Evolution and Arabidopsis Mutant Analysis

    Directory of Open Access Journals (Sweden)

    Pengkai Wang

    2016-09-01

    Full Text Available The APETALA2 (AP2 genes represent the AP2 group within a large group of DNA-binding proteins called AP2/EREBP. The AP2 gene is functional and necessary for flower development, stem cell maintenance, and seed development, whereas the other members of AP2 group redundantly affect flowering time. Here we study the phylogeny of AP2 group genes in spermatophytes. Spermatophyte AP2 group genes can be classified into AP2 and TOE types, six clades, and we found that the AP2 group homologs in gymnosperms belong to the AP2 type, whereas TOE types are absent, which indicates the AP2 type gene are more ancient and TOE type was split out of AP2 type and losing the major function. In Brassicaceae, the expansion of AP2 and TOE type lead to the gene number of AP2 group were up to six. Purifying selection appears to have been the primary driving force of spermatophyte AP2 group evolution, although positive selection occurred in the AP2 clade. The transition from exon to intron of AtAP2 in Arabidopsis mutant leads to the loss of gene function and the same situation was found in AtTOE2. Combining this evolutionary analysis and published research, the results suggest that typical AP2 group genes may first appear in gymnosperms and diverged in angiosperms, following expansion of group members and functional differentiation. In angiosperms, AP2 genes (AP2 clade inherited key functions from ancestors and other genes of AP2 group lost most function but just remained flowering time controlling in gene formation. In this study, the phylogenies of AP2 group genes in spermatophytes was analyzed, which supported the evidence for the research of gene functional evolution of AP2 group.

  17. Constitutive Expression of Sense & Antisense PtAP3, an AP3 Homologue Gene of Populus tomentosa, Affects Growth and Flowering Time in Transgenic Tobacco

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    To analyze the function of PtAP3, an APETALA3 (AP3) homologue gene isolated from Populus tomentosa Carr., the full length sequence (1 797 bp) and a fragment (870 bp) of PtAP3 were fused to a CaMV 35S promoter of pBI121 to generate the sense and antisense constructs of PtAP3. These constructs were transformed into tobacco by Agrobacterium infection of leaf disks and selection on kanamycin medium. Some sense and antisense transgenic tobacco plants were obtained by PCR and Southern blot analysis. Great phenotypic differences in transgenic tobacco plants were observed. Almost all of sense PtAP3 to transgenic tobaccos showed a higher growth rate than those of antisense transformants and a few developed pregnancy earlier than wild type seedlings and antisense transformants under the same conditions.

  18. RAD51AP2, a novel vertebrate- and meiotic-specific protein, sharesa conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51

    Energy Technology Data Exchange (ETDEWEB)

    Kovalenko, Oleg V.; Wiese, Claudia; Schild, David

    2006-07-25

    Many interacting proteins regulate and/or assist the activities of RAD51, a recombinase which plays a critical role in both DNA repair and meiotic recombination. Yeast two-hybrid screening of a human testis cDNA library revealed a new protein, RAD51AP2 (RAD51 Associated Protein 2), that interacts strongly with RAD51. A full-length cDNA clone predicts a novel vertebrate specific protein of 1159 residues, and the RAD51AP2 transcript was observed only in meiotic tissue (i.e. adult testis and fetal ovary), suggesting a meiotic-specific function for RAD51AP2. In HEK293 cells the interaction of RAD51 with an ectopically-expressed recombinant large fragment of RAD51AP2 requires the C-terminal 57 residues of RAD51AP2. This RAD51-binding region shows 81% homology to the C-terminus of RAD51AP1/PIR51, an otherwise totally unrelated RAD51-binding partner that is ubiquitously expressed. Analyses using truncations and point mutations in both RAD51AP1 and RAD51AP2 demonstrate that these proteins use the same structural motif for RAD51 binding. RAD54 shares some homology with this RAD51-binding motif, but this homologous region plays only an accessory role to the adjacent main RAD51-interacting region, which has been narrowed here to 40 amino acids. A novel protein, RAD51AP2, has been discovered that interacts with RAD51 through a C-terminal motif also present in RAD51AP1.

  19. Duplication of AP1 within the Spinacia oleracea L. AP1/FUL clade is followed by rapid amino acid and regulatory evolution.

    Science.gov (United States)

    Sather, D Noah; Golenberg, Edward M

    2009-02-01

    The AP1/FUL clade of MADS box genes have undergone multiple duplication events among angiosperm species. While initially identified as having floral meristem identity and floral organ identity function in Arabidopsis, the role of AP1 homologs does not appear to be universally conserved even among eudicots. In comparison, the role of FRUITFULL has not been extensively explored in non-model species. We report on the isolation of three AP1/FUL genes from cultivated spinach, Spinacia oleracea L. Two genes, designated SpAPETALA1-1 (SpAP1-1) and SpAPETALA1-2 (SpAP1-2), cluster as paralogous genes within the Caryophyllales AP1 clade. They are highly differentiated in the 3', carboxyl-end encoding region of the gene following the third amphipathic alpha-helix region, while still retaining some elements of a signature AP1 carboxyl motifs. In situ hybridization studies also demonstrate that the two paralogs have evolved different temporal and spatial expression patterns, and that neither gene is expressed in the developing sepal whorl, suggesting that the AP1 floral organ identity function is not conserved in spinach. The spinach FRUITFULL homolog, SpFRUITFULL (SpFUL), has retained the conserved motif and groups with Caryophyllales FRUITFULL homologs. SpFUL is expressed in leaf as well as in floral tissue, and shows strong expression late in flower development, particularly in the tapetal layer in males, and in the endothecium layer and stigma, in the females. The combined evidence of high rates of non-synonymous substitutions and differential expression patterns supports a scenario in which the AP1 homologs in the spinach AP1/FUL gene family have experienced rapid evolution following duplication.

  20. Masas de agujeros negros en Narrow Line Seyfert 1

    Science.gov (United States)

    Schmidt, E.; Ferreiro, D.; Oio, G.; Vega, L.; Donoso, L.

    We describe two of the ways to estimate black hole masses in AGN, and then we estimate the black hole masses of 13 Narrow Line Seyfert 1 galaxies with the two methods: virial masses, using the correlation found by Greene & Ho (2005, ApJ, 630, 122); and the correlation found by Tremaine et al. (2002, ApJ, 574, 740). For this work we analyzed the optical spectroscopy data we obtained from CASLEO (San Juan). We compare the results obtained through both methods. FULL TEXT IN SPANISH

  1. Traffic Analysis Zones - TRAFFIC_COUNTS_INDOTMODEL_IN: Traffic Counts on Roadways (from INDOT Statewide Travel Demand MODEL version 4, ISTDM4) in Indiana (Indiana Department of Transportation, Line Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — TRAFFIC_COUNTS_INDOTMODEL_IN is a line shapefile that shows traffic counts (factored to year 2000) for the roadways in the Indiana Statewide Travel Demand Model,...

  2. Loss of p12CDK2-AP1 Expression in Human Oral Squamous Cell Carcinoma with Disrupted Transforming Growth Factor-β-Smad Signaling Pathway

    Directory of Open Access Journals (Sweden)

    Hui Peng

    2006-12-01

    Full Text Available We examined correlations between TGF-β1, TβR-I and TβR-II, p12CDK2-AP1 p21WAF1 p27KIP1 Smad2, and p-Smad2 in 125 cases of human oral squamous cell carcinoma (OSCC to test the hypothesis that resistance to TGF-β1-induced growth suppression is due to the disruption of its signaling pathway as a consequence of reduced or lost p12CDK2-AP1. Immunoreactivity for TβR-II decreased in OSCC with increasing disease aggressiveness; however, no differences were observed for TβR-I and TGF-β1. The expression of TβR-II significantly correlated with p12CDK2-AP1 and p27KIP1 (P<.001 and P<.01, respectively. Furthermore, there was a significant relationship between TβR-II expression and p-Smad2 (P < .001. The in vivo correlation of the levels of TβR-II, p12CDK2-AP1 and p27 KIP1 was confirmed in normal and OSCC cell lines. Additionally, in vitro analysis of TGF-β-treated cells showed that TGF-β1 treatment of normal keratinocytes suppressed cell growth with upregulation of p-Smad2, p12CDK2-API and p21WAF1 expression, whereas there was no effect on OSCC cell lines. These results provide evidence of a link between a disrupted TGF-β-Smad signaling pathway and loss of induction of cell cycle-inhibitory proteins, especially p12CDK2-AP1 in OSCC, which may lead to the resistance of TGF-β1 growth-inhibitory effect on OSCC.

  3. Neurotransmitter transporters

    DEFF Research Database (Denmark)

    Gether, Ulrik; Andersen, Peter H; Larsson, Orla M

    2006-01-01

    The concentration of neurotransmitters in the extracellular space is tightly controlled by distinct classes of membrane transport proteins. This review focuses on the molecular function of two major classes of neurotransmitter transporter that are present in the cell membrane of neurons and....../or glial cells: the solute carrier (SLC)1 transporter family, which includes the transporters that mediate the Na(+)-dependent uptake of glutamate, and the SLC6 transporter family, which includes the transporters that mediate the Na(+)-dependent uptake of dopamine, 5-HT, norepinephrine, glycine and GABA....... Recent research has provided substantial insight into the structure and function of these transporters. In particular, the recent crystallizations of bacterial homologs are of the utmost importance, enabling the first reliable structural models of the mammalian neurotransmitter transporters...

  4. 转基因白桦杂种T1代的生长发育及AP1基因的遗传分析%Growth and developmental analysis of T1 generation from BpAP1 transgenic birch

    Institute of Scientific and Technical Information of China (English)

    王朔; 黄海娇; 杨光; 姜静; 刘桂丰

    2016-01-01

    Woody plants are characterized by long developing period and high heterozygosity. It is important to shorten the life cycle of trees in tree breeding. APETALA1 (AP1) is a member of MADS-box gene family involved in flower development in plants. Overexpression of AP1 genes induces early flowering in transgenic plants. In order to investigate the genetic stability and regularity of foreign BpAP1 gene in BpAP1 transgenic birch, we generated T1 generation seedlings using three 35S BpAP1 transgenic plants and one wild-type plant as male parents, and three wild-type birch, Betula platyphylla í Betula pendula, as female parents. The growth, development and flowering characteristics of the T1 generation seedlings were analyzed. The results indicated that the foreign BpAP1 slightly influenced the pollen vitality of transgenic plants. About 36% -58% of T1 generation inherited the foreign BpAP1 gene from their parents. Chi-square test of BpAP1 gene segregation ratios revealed that BpAP1 was inherited in accordance with Mendelian inheritance. T1 generation seedlings carrying BpAP1 gene inherited the characteristics of early flowering and dwarfism from their male parent. The average heights of 1-and 2-year-old T1 generation seedlings carrying BpAP1 gene were significantly shorter than the progeny from hybridization of wild-type birch with the percentages of 44. 19% and 18. 92%, respectively. The phenotypes of T1 generation birch carrying foreign BpAP1 gene were quite different from the ones that were lack of foreign BpAP1 gene. According to the different phenotypes, we can infer whether the T1 generation seedlings carry BpAP1 gene or not. Our study proved that the exogenous AP1 gene can be stably inherited by sexual reproduction, and the acquired transgenic birch lines exhibited accelerated flowering time and a shortened juvenile phase, indicating that it can be used as parent materials for genetic studies on birch traits.%本文以3株野生型白桦为母本、3株35S宜BpAP

  5. School Transportation.

    Science.gov (United States)

    Executive Educator, 1990

    1990-01-01

    This special section on student transportation offers a case study of a school system that recycles buses for safety drills; articles on fuel-saving strategies, the pros and cons of contracting for transportation services or operating a publicly owned bus fleet, and advice on full cost accounting for transportation costs; and a transportation…

  6. Study on the Transportation Organization Plan of Shenyang Metro Line 2 During the 12th National Games%沈阳地铁二号线运输组织方案优化研究--以第十二届全运会期间为例

    Institute of Scientific and Technical Information of China (English)

    刘小玲; 薛亮

    2014-01-01

    分析了沈阳地铁二号线断面客流的时空分布特征和运力匹配情况,提出了二号线在第十二届全运会期间存在的高峰期运力不足、上下行不均衡程度大等问题。根据全运会期间的客流特征,提出了全运会期间地铁二号线运输组织方案的优化研究。%With an analysis of the sectio n passenger flow distribution features of time space continuum, the matching condition between planning capacity and the actual transport volume of Shenyang metro Line 2, this article brings forward some potential problems of Line 2 during the 12th national games, such as the deficiency of train capacity in peak times, the serious imbalance of up and town traffic, and so on. The op-timization research on transportation organization plan of Line 2 during 2013 national games is presented according to the passenger character during the national games.

  7. AP fuels and the potential of renewable diesel

    Energy Technology Data Exchange (ETDEWEB)

    Berkley, Mark; Seifkar, Navid; O' Shea, Michael; Peters, Christopher

    2010-09-15

    The decrease in demand for forestry products has been detrimental to the Province of Quebec's industrial base. With increasing energy security and environmental concerns the promotion of innovative technologies is adamant. AP Fuels Inc. has undertaken the development of a biomass-to-liquids facility proposed herein as a hybrid design, combining biomass and natural gas capable of producing diesel and other liquid fuels. The facility would consume 2,200,000 tonnewet per year of biomass and produce 10,600 bbl/day of liquid fuels. Forestry-derived F-T fuels have notable advantages including: improved performance; ultra-low sulphur content; reduced emissions, particulates and fouling; and production of fewer by-products.

  8. Aerosol Polarimetry Sensor (APS): Design Summary, Performance and Potential Modifications

    Science.gov (United States)

    Cairns, Brian

    2014-01-01

    APS is a mature design that has already been built and has a TRL of 7. Algorithmic and retrieval capabilities continue to improve and make better and more sophisticated used of the data. Adjoint solutions, both in one dimensional and three dimensional are computationally efficient and should be the preferred implementation for the calculation of Jacobians (one dimensional), or cost-function gradients (three dimensional). Adjoint solutions necessarily provide resolution of internal fields and simplify incorporation of active measurements in retrievals, which will be necessary for a future ACE mission. Its best to test these capabilities when you know the answer: OSSEs that are well constrained observationally provide the best place to test future multi-instrument platform capabilities and ensure capabilities will meet scientific needs.

  9. Towards a theory of rapidly oscillating Ap stars

    CERN Document Server

    Gough, Douglas

    2011-01-01

    Peculiar A stars are so named because they exhibit abundance peculiarities in their atmospheres. It is believed that these arise as a result of differentiation of chemical species in large magnetic spots in which convective mixing is inhibited: there might be just two antipodal spots, whose axis is inclined to the axis of rotation. Many of the Ap stars that are rotating slowly also pulsate, with periods substantially shorter than the period of the fundamental radial mode. The pulsations appear to be nonradial, but axisymmetric, with their common axis usually aligned with the axis of the spots. In this lecture I shall first discuss the magnetic suppression of convection in the spots, and then I shall try to explain the pulsation phenomenon, reviewing some of the suggestions that have been made to explain the alignment and the excitation mechanism, and finally raising some issues that need to be addressed.

  10. Fabrication and Testing of Deflecting Cavities for APS

    Energy Technology Data Exchange (ETDEWEB)

    Mammosser, John; Wang, Haipeng; Rimmer, Robert; Jim, Henry; Katherine, Wilson; Dhakal, Pashupati; Ali, Nassiri; Jim, Kerby; Jeremiah, Holzbauer; Genfa, Wu; Joel, Fuerst; Yawei, Yang; Zenghai, Li

    2013-09-01

    Jefferson Lab (Newport News, Virginia) in collaboration with Argonne National Laboratory (Argonne, IL) has fabricated and tested four first article, 2.8 GHz, deflecting SRF cavities, for Argonne's Short-Pulse X-ray (SPX) project. These cavities are unique in many ways including the fabrication techniques in which the cavity cell and waveguides were fabricated. These cavity subcomponents were milled from bulk large grain niobium ingot material directly from 3D CAD files. No forming of sub components was used with the exception of the beam-pipes. The challenging cavity and helium vessel design and fabrication results from the stringent RF performance requirements required by the project and operation in the APS ring. Production challenges and fabrication techniques as well as testing results will be discussed in this paper.

  11. Aneurisma gigante del apéndice auricular izquierdo

    OpenAIRE

    Francisco L. Moreno-Martínez; González Alfonso,Osvaldo; Lagomasino Hidalgo,Álvaro L; González Díaz,Alejandro; Oliva Céspedes,Carlos; López Bernal,Omaida J

    2006-01-01

    Los aneurismas de la aurícula izquierda son raros y pueden ser congénitos o adquiridos. Los que interesan la pared libre o el apéndice auricular son entidades más raras aún, hasta 2002 sólo existían 49 casos reportados en la aurícula izquierda y 8 en la derecha. La manifestación clínica más frecuente es la aparición de arritmias auriculares incesantes o recurrentes y pueden presentarse embolias sistémicas que pueden dar al traste con la vida del paciente. Presentamos el caso de una paciente a...

  12. Measurement of sextupole orbit offsets in the APS storage ring.

    Energy Technology Data Exchange (ETDEWEB)

    Borland, M.

    1999-04-16

    Horizontal orbit errors at the sextuples in the Advanced Photon Source (APS) storage ring can cause changes in tune and modulation of the beta functions around the ring. To determine the significance of these effects requires knowing the orbit relative to the magnetic center of the sextuples. The method considered here to determine the horizontal beam position in a given sextupole is to measure the tune shift caused by a change in the sextupole strength. The tune shift and a beta function for the same plane uniquely determine the horizontal beam position in the sextupole. The beta function at the sextupole was determined by propagating the beta functions measured at nearby quadrupoles to the sextupole location. This method was used to measure the sextupole magnetic center offset relative to an adjacent beam position monitor (BPM) at a number of sextupole locations. We report on the successes and problems of the method as well as an improved method.

  13. Enhanced Antimicrobial Activity of AamAP1-Lysine, a Novel Synthetic Peptide Analog Derived from the Scorpion Venom Peptide AamAP1

    Directory of Open Access Journals (Sweden)

    Ammar Almaaytah

    2014-04-01

    Full Text Available There is great interest in the development of antimicrobial peptides as a potentially novel class of antimicrobial agents. Several structural determinants are responsible for the antimicrobial and cytolytic activity of antimicrobial peptides. In our study, a new synthetic peptide analog, AamAP1-Lysine from the naturally occurring scorpion venom antimicrobial peptide AamAP1, was designed by modifying the parent peptide in order to increase the positive charge and optimize other physico-chemical parameters involved in antimicrobial activity. AamAP1-Lysine displayed potent antibacterial activity against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration was in the range of 5 to 15 µM with a 10 fold increase in potency over the parent peptide. The hemolytic and antiproliferative activity of AamAP1-Lysine against eukaryotic mammalian cells was minimal at the concentration range needed to inhibit bacterial growth. The antibacterial mechanism analysis indicated that AamAP1-Lysine is probably inducing bacterial cell death through membrane damage and permeabilization determined by the release of β-galactosidase enzyme from peptide treated E. coli cells. DNA binding studies revealed that AamAP1-Lysine caused complete retardation of DNA migration and could display intracellular activities in addition to the membrane permeabilization mode of action reported earlier. In conclusion, AamAP1-Lysine could prove to be a potential candidate for antimicrobial drug development in future studies.

  14. Sustainable Transportation

    DEFF Research Database (Denmark)

    Hall, Ralph P.; Gudmundsson, Henrik; Marsden, Greg

    2014-01-01

    The transportation system is the backbone of economic and social progress and the means by which humans access goods and services and connect with one another. Yet, as the scale of transportation activities has grown worldwide, so too have the negative environmental, social, and economic impacts...... that relate to the construction and maintenance of transportation infrastructure and the operation or use of the different transportation modes. The concept of sustainable transportation emerged in response to these concerns as part of the broader notion of sustainable development. Given the transportation...... sector’s significant contribution to global challenges such as climate change, it is often said that sustainable development cannot be achieved without sustainable transportation....

  15. 无线网络钓鱼 AP 攻击检测技术研究%RESEARCH ON DETECTION TECHNOLOGY OF FISHING AP ATTACK IN WIRELESS NETWORK

    Institute of Scientific and Technical Information of China (English)

    金双齐; 凌捷

    2016-01-01

    With the popularisation of WLAN,the attacks against wireless network are increasingly growing.Wireless fishing AP attack, through passive or active way,induces users to connect fishing AP,and then catches users’sensitive information,it is currently one of the abused attack modes.In light of this,we proposed an improved fishing AP attack detection method,by using diminishing variation of TTL value as well as comprehensively analysing the gateway and routing information,it realises validity detection on AP.Experimental results showed that this method can effectively detect the attacks including wireless fishing AP and wireless man-in-the-middle.%随着无线局域网应用的普及,针对无线网络的攻击方式也逐渐增多。无线钓鱼 AP 攻击通过被动或主动方式诱使用户连接钓鱼 AP,进而获取用户的敏感信息,是当前被滥用的攻击方式之一。针对这种情况,提出一种改进的钓鱼 AP 攻击检测方法,通过利用 TTL 值的递减变化,以及综合分析网关与路由信息,实现对 AP 的合法性检测。实验结果表明,该方法能够有效地检测无线钓鱼 AP 和无线中间人等攻击。

  16. Molecular characterization and specific detection of Anaplasma species (AP-sd) in sika deer and its first detection in wild brown bears and rodents in Hokkaido, Japan.

    Science.gov (United States)

    Moustafa, Mohamed Abdallah Mohamed; Lee, Kyunglee; Taylor, Kyle; Nakao, Ryo; Sashika, Mariko; Shimozuru, Michito; Tsubota, Toshio

    2015-12-01

    A previously undescribed Anaplasma species (herein referred to as AP-sd) has been detected in sika deer, cattle and ticks in Japan. Despite being highly similar to some strains of A. phagocytophilum, AP-sd has never been detected in humans. Its ambiguous epidemiology and the lack of tools for its specific detection make it difficult to understand and interpret the prevalence of this Anaplasma species. We developed a method for specific detection, and examined AP-sd prevalence in Hokkaido wildlife. Our study included 250 sika deer (Cervus nippon yesoensis), 13 brown bears (Ursus arctos yesoensis) and 252 rodents including 138 (Apodemus speciosus), 45 (Apodemus argenteus), 42 (Myodes rufocanus) and 27 (Myodes rutilus) were collected from Hokkaido island, northern Japan, collected during 2010 to 2015. A 770 bp and 382 bp segment of the 16S rRNA and gltA genes, respectively, were amplified by nested PCR. Results were confirmed by cloning and sequencing of the positive PCR products. A reverse line blot hybridization (RLB) based on the 16S rRNA gene was then developed for the specific detection of AP-sd. The prevalence of AP-sd by nested PCR in sika deer was 51% (128/250). We detected this Anaplasma sp. for the first time in wild brown bears and rodents with a prevalence of 15% (2/13) and 2.4% (6/252), respectively. The sequencing results of the 16S rRNA and gltA gene amplicons were divergent from the selected A. phagocytophilum sequences in GenBank. Using a newly designed AP-sd specific probe for RLB has enabled us to specifically detect this Anaplasma species. Besides sika deer and cattle, wild brown bears and rodents were identified as potential reservoir hosts for AP-sd. This study provided a high throughput molecular method that specifically detects AP-sd, and which can be used to investigate its ecology and its potential as a threat to humans in Japan.

  17. The Role of JNK and p38 MAPK Activities in UVA-Induced Signaling Pathways Leading to AP-1 Activation and c-Fos Expression

    Directory of Open Access Journals (Sweden)

    Amy L. Silvers

    2003-07-01

    Full Text Available To further delineate ultraviolet A (UVA signaling pathways in the human keratinocyte cell line HaCaT, we examined the potential role of mitogen-activated protein kinases (MAPKs in UVA-induced activator protein-1 (AP-1 transactivation and c-Fos expression. UVA-induced phosphorylation of p38 and c-Jun N-terminal kinase (JNK proteins was detected immediately after irradiation and disappeared after approximately 2 hours. Conversely, phosphorylation of extracellular signal-regulated kinase was significantly inhibited for up to 1 hour post-UVA irradiation. To examine the role of p38 and JNK MAPKs in UVA-induced AP-1 and c-fos transactivations, the selective pharmacologic MAPK inhibitors, SB202190 (p38 inhibitor and SP600125 (JNK inhibitor, were used to independently treat stably transfected HaCaT cells in luciferase reporter assays. Both SB202190 and SP600125 dose-dependently inhibited UVA-induced AP-1 and c-fos transactivations. SB202190 (0.25–0.5 MM and SP600125 (62-125 nM treatments also primarily inhibited UVA-induced c-Fos expression. These results demonstrated that activation of both JNK and p38 play critical role in UVA-mediated AP-1 transactivation and c-Fos expression in these human keratinocyte cells. Targeted inhibition of these MAPKs with their selective pharmacologic inhibitors may be effective chemopreventive strategies for UVA-induced nonmelanoma skin cancer.

  18. Study on the thermal decomposition behavior of RDX/AP/HTPB propellant%RDX/AP/HTPB推进剂热分解特性研究

    Institute of Scientific and Technical Information of China (English)

    陈沛; 赵凤起; 阴翠梅

    2002-01-01

    利用高压差示扫描量热仪(PDSC)研究了RDX/AP/HTPB推进剂系列配方的热分解性能,发现配方组分的改变对RDX/AP/HTPB推进剂的热分解性能有影响,突出表现在RDX/AP/HTPB推进剂中RDX分解峰变宽,AP放热分解效应增强.推进剂中添加Al粉后,RDX的分解受到抑制,而AP的分解却得到增强.

  19. HPV16 E6 and E6AP differentially cooperate to stimulate or augment Wnt signaling

    Energy Technology Data Exchange (ETDEWEB)

    Sominsky, Sophia, E-mail: sophia.tab@gmail.com [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Kuslansky, Yael, E-mail: ykuslansky@gmail.com [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Shapiro, Beny, E-mail: benyshap@gmail.com [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Jackman, Anna, E-mail: jackman@post.tau.ac.il [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Haupt, Ygal, E-mail: ygal.haupt@petermac.org [Research Division, The Peter MacCallum Cancer Centre, East Melbourne (Australia); Rosin-Arbesfeld, Rina, E-mail: arina@post.tau.ac.il [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Sherman, Levana, E-mail: lsherman@post.tau.ac.il [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel)

    2014-11-15

    The present study investigated the roles of E6 and E6AP in the Wnt pathway. We showed that E6 levels are markedly reduced in cells in which Wnt signaling is activated. Coexpression of wild-type or mutant E6AP (C820A) in Wnt-activated cells stabilized E6 and enhanced Wnt/β-catenin/TCF transcription. Expression of E6AP alone in nonstimulated cells elevated β-catenin level, promoted its nuclear accumulation, and activated β-catenin/TCF transcription. A knockdown of E6AP lowered β-catenin levels. Coexpression with E6 intensified the activities of E6AP. Further experiments proved that E6AP/E6 stabilize β-catenin by protecting it from proteasomal degradation. This function was dependent on the catalytic activity of E6AP, the kinase activity of GSK3β and the susceptibility of β-catenin to GSK3β phosphorylation. Thus, this study identified E6AP as a novel regulator of the Wnt signaling pathway, capable of cooperating with E6 in stimulating or augmenting Wnt/β-catenin signaling, thereby possibly contributing to HPV carcinogenesis. - Highlights: • The roles of E6 and E6AP in the Wnt pathway were investigated. • E6AP stabilizes E6 and enhances E6 activity in augmentation of Wnt signaling. • E6AP cooperates with E6 to stabilize β-catenin and stimulate Wnt/β-catenin signaling. • E6AP and E6 act through different mechanisms to augment or stimulate Wnt signaling.

  20. On the evolution of the magnetic field of Ap star $\\alpha^2$ CVn

    CERN Document Server

    Bychkov, V D; Madej, J; Topilskaya, G P

    2016-01-01

    New high-precision measurements of the longitudinal magnetic field of Ap stars suggest the existence of secular intrinsic variations of the global magnetic field in some stars. We argue that such changes are apparent in the Ap star $\\alpha^2$ CVn in the time scale of $\\sim$ 10 years, which results from the analysis of literature data. Therefore, such an observation implies, that the rate of magnetic field evolution of Ap stars is much higher than was previously thought.

  1. Coupling sequential injection on-line preconcentration by means of a renewable microcolumn with ion-exchange beads with detection by electrothermal atomic absorption spectrometry. Comparing the performance of eluting the loaded beads with transporting them directly into the graphite tube

    DEFF Research Database (Denmark)

    Wang, Jianhua; Hansen, Elo Harald

    2001-01-01

    The design of a flow injection/sequential injection (FIA/SIA) on-line preconcentration system incorporating a renewable microcolumn with ion-exchange beads and interfaced with an electrothermal atomic absorption spectrometry (ETAAS) detector is described, and its practical applicability.......4% for the procedure in which the loaded beads are transported directly to the graphite furnace for pyrolysis and atomization, and even improved in comparison to the traditional unidirectional and bidirectional repetitive elution procedures which under comparable conditions yield R.S.D.-values of 5.8 and 4...

  2. LBB在AP1000技术中的应用%The Application of LBB in AP1000 Technology

    Institute of Scientific and Technical Information of China (English)

    蒋冬梅; 杜颖; 袁小兰

    2015-01-01

    压水堆核电厂的设计基准事故定义为主管道双端断裂,这一假设过于保守.管道的失效方式往往是先出现破口而后再泄漏,即所谓的先漏后破( Leak Before Break,简称LBB) ,而不是双端断裂. LBB准则是防止核电厂压力管道发生灾难性破裂事故的重要评定准则.本文介绍了LBB评定技术在国外的发展情况和意义,阐述了LBB在AP1000核电厂中的应用情况及LBB评价的理论基础.%Design basis accident of pressurized water reactor nuclear power plant was de-fined as main pipeline double-end fracture, this assumption is too conservative. Pipeline failure modes often appear as first Break then Leak,that is so-called the Leak Before Break ( Leak Before Break,LBB) ,rather than double end fracture. LBB is an important evalua-tion criteria of preventing pressure piping catastrophic rupture accident of nuclear power plant . This paper briefly introduces the development situation and the significance of the o-verseas LBB assessment technology and expounds the application of LBB in AP1000 nucle-ar power plant and the theoretical basis of LBB assessment.

  3. 49 CFR 192.709 - Transmission lines: Record keeping.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Transmission lines: Record keeping. 192.709... TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Maintenance § 192.709 Transmission lines: Record keeping. Each operator shall maintain the following records for transmission...

  4. 49 CFR 179.201-3 - Lined tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Lined tanks. 179.201-3 Section 179.201-3... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.201-3 Lined tanks. (a)...

  5. K-AP: Generating specified K clusters by efficient Affinity Propagation

    KAUST Repository

    Zhang, Xiangliang

    2010-12-01

    The Affinity Propagation (AP) clustering algorithm proposed by Frey and Dueck (2007) provides an understandable, nearly optimal summary of a data set. However, it suffers two major shortcomings: i) the number of clusters is vague with the user-defined parameter called self-confidence, and ii) the quadratic computational complexity. When aiming at a given number of clusters due to prior knowledge, AP has to be launched many times until an appropriate setting of self-confidence is found. The re-launched AP increases the computational cost by one order of magnitude. In this paper, we propose an algorithm, called K-AP, to exploit the immediate results of K clusters by introducing a constraint in the process of message passing. Through theoretical analysis and experimental validation, K-AP was shown to be able to directly generate K clusters as user defined, with a negligible increase of computational cost compared to AP. In the meanwhile, K-AP preserves the clustering quality as AP in terms of the distortion. K-AP is more effective than k-medoids w.r.t. the distortion minimization and higher clustering purity. © 2010 IEEE.

  6. A Weighted K-AP Query Method for RSSI based Indoor Positioning

    DEFF Research Database (Denmark)

    Huo, Huan; Liu, Xiufeng; Li, Jifeng

    2016-01-01

    The paper studies the establishment of offline fingerprint library based on RSSI (Received Signal Strength Indication), and proposes WF-SKL algorithm by introducing the correlation between RSSIs. The correlations can be transformed as AP fingerprint sequence to build the offline fingerprint library....... To eliminate the positioning error caused by instable RSSI value, WF-SKL can filter the noise AP via online AP selection, meanwhile it also reduces the computation load. WF-SKL utilizes LCS algorithm to find out the measurement between the nearest neighbors, and it proposes K-AP (P,Q) nearest neighbor queries...

  7. Isolation, classification and transcription profiles of the AP2/ERF transcription factor superfamily in citrus.

    Science.gov (United States)

    Xie, Xiu-lan; Shen, Shu-ling; Yin, Xue-ren; Xu, Qian; Sun, Chong-de; Grierson, Donald; Ferguson, Ian; Chen, Kun-song

    2014-07-01

    The AP2/ERF gene family encodes plant-specific transcription factors. In model plants, AP2/ERF genes have been shown to be expressed in response to developmental and environmental stimuli, and many function downstream of the ethylene, biotic, and abiotic stress signaling pathways. In citrus, ethylene is effective in regulation citrus fruit quality, such as degreening and aroma. However, information about the citrus AP2/ERF family is limited, and would enhance our understanding of fruit responses to environmental stress, fruit development and quality. CitAP2/ERF genes were isolated using the citrus genome database, and their expression patterns analyzed by real-time PCR using various orange organs and samples from a fruit developmental series. 126 sequences with homologies to AP2/ERF proteins were identified from the citrus genome, and, on the basis of their structure and sequence, assigned to the ERF family (102), AP2 family (18), RAV family (4) and Soloist (2). MEME motif analysis predicted the defining AP2/ERF domain and EAR repressor domains. Analysis of transcript accumulation in Citrus sinensis cv. 'Newhall' indicated that CitAP2/ERF genes show organ-specific and temporal expression, and provided a framework for understanding the transcriptional regulatory roles of AP2/ERF gene family members in citrus. Hierarchical cluster analysis and t tests identified regulators that potentially function during orange fruit growth and development.

  8. Heterologous expression in Tritrichomonas foetus of functional Trichomonas vaginalis AP65 adhesin

    Directory of Open Access Journals (Sweden)

    Alderete JF

    2005-03-01

    Full Text Available Abstract Background Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs, a step preparatory to infection, is mediated in part by the prominent surface protein AP65. The bovine trichomonad, Tritrichomonas foetus, adheres poorly to human VECs. Thus, we established a transfection system for heterologous expression of the T. vaginalis AP65 in T. foetus, as an alternative approach to confirm adhesin function for this virulence factor. Results In this study, we show stable transfection and expression of the T. vaginalis ap65 gene in T. foetus from an episomal pBS-ap65-neo plasmid. Expression of the gene and protein was confirmed by RT-PCR and immunoblots, respectively. AP65 in transformed T. foetus bound to host cells. Specific mAbs revealed episomally-expressed AP65 targeted to the parasite surface and hydrogenosome organelles. Importantly, surface-expression of AP65 in T. foetus paralleled increased levels of adherence of transfected bovine trichomonads to human VECs. Conclusion The T. vaginalis AP65 adhesin was stably expressed in T. foetus, and the data obtained using this heterologous system strongly supports the role of AP65 as a prominent adhesin for T. vaginalis. In addition, the heterologous expression in T. foetus of a T. vaginalis gene offers an important, new approach for confirming and characterizing virulence factors.

  9. Differential role of SH2-B and APS in regulating energy and glucose homeostasis.

    Science.gov (United States)

    Li, Minghua; Ren, Decheng; Iseki, Masanori; Takaki, Satoshi; Rui, Liangyou

    2006-05-01

    SH2-B and APS, two members of a pleckstrin homology and SH2 domain-containing adaptor family, promote both insulin and leptin signaling in a similar fashion in cultured cells. In addition, APS mediates insulin-stimulated activation of the c-Cbl/CAP/TC10 pathway in cultured adipocytes. Here we characterized genetically modified mice lacking SH2-B, APS, or both to determine the physiological roles of these two proteins in animals. Disruption of the SH2-B gene resulted in obesity, hyperglycemia, hyperinsulinemia, and glucose intolerance. Conversely, deletion of the APS gene did not alter adiposity, energy balance, and glucose metabolism. Energy intake, energy expenditure, fat content, body weight, and plasma insulin, leptin, glucose, and lipid levels were similar between APS(-/-) and WT littermates fed either normal chow or a high-fat diet. Moreover, deletion of APS failed to alter insulin and glucose tolerance. APS(-/-)/SH2-B(-/-) double knockout mice also developed energy imbalance, obesity, hyperleptinemia, hyperinsulinemia, hyperglycemia, and glucose intolerance; however, plasma leptin and insulin levels were significantly lower in APS(-/-)/SH2-B(-/-) than in SH2-B(-/-) mice. These results suggest that SH2-B, but not APS, is a key positive regulator of energy and glucose metabolism in mice.

  10. APS-U Definitions of Signs and Conventions Related to Magnets

    Energy Technology Data Exchange (ETDEWEB)

    Doose, Charles [Argonne National Lab. (ANL), Argonne, IL (United States). Accelerator Systems Division; Jain, Animesh [Brookhaven National Lab. (BNL), Upton, NY (United States). Superconducting Magnet Division

    2014-08-21

    The APS-U is planned to be a 4th generation hard X-ray light source utilizing a multi-bend achromat (MBA) magnet lattice. The MBA lattice will be installed in the existing APS storage ring enclosure. The stored electron beam will circulate clockwise when viewed from above. The X-ray beamlines will for the most part exit at the same source points as the present APS. This document defines the signs and conventions related to the APS-U MBA magnets. Included in this document are: the local magnet coordinate system, definitions of mechanical and magnetic centers, definitions of multipole field errors, magnetic roll angle, and magnet polarities.

  11. Selection of first-line i.v. antibiotics for acute pyelonephritis in patients requiring emergency hospital admission.

    Science.gov (United States)

    Yasufuku, Tomihiko; Shigemura, Katsumi; Yamashita, Masuo; Arakawa, Soichi; Fujisawa, Masato

    2011-04-01

    Febrile urinary tract infections (UTIs) often require the intravenous infusion of antibiotics and/or hospitalization. Acute pyelonephritis (AP) is one of the most severe forms of UTI, and the antibiotics we should use as the first line and the risk factors for treatment failure remain controversial. The objective of this study was to investigate the efficacy of i.v. antibiotics selected for the treatment of febrile AP and to examine the risk factors for antibiotic resistance. We set risk factors for antibiotic treatment failure such as age, sex, and the presence of underlying urinary tract disease. We classified all cases into 49 cases of complicated AP and 24 cases of uncomplicated AP according to the presence of underlying urinary tract diseases, and examined the characteristics of the patients and the efficacy of the antibiotics used in this study. We investigated risk factors which relate to initial treatment failure and the duration of antibiotic treatment. Initial antibiotic treatment failure was significantly correlated to C-reactive protein in complicated AP and to positive blood culture in uncomplicated AP. We revealed a significant correlation between the duration of the given antibiotics and diabetes mellitus or positive blood culture in uncomplicated AP, and tazobactam/piperacillin was significantly related to prolongation of antibiotic treatment in complicated AP. In conclusion, in this study, a positive blood culture was the representative risk factor that related to both initial treatment failure and longer duration of the given antibiotics in uncomplicated AP.

  12. Moisture Transport for Reaction Enhancement in Fabrics

    Science.gov (United States)

    2013-01-01

    Figure 1. Equal gas flows were 4 Journal of Textiles Wicking glass fiber filter Reactive polymer membrane laminate W at er v ap or fl ux (g /s...quickly through the laminate , and a higher total amount is transported (area under the curve) 6 Journal of Textiles (a) eVent ePTFE film (b) Gore-Tex...transported through the membrane is equivalent for the two laminates . It just takes a longer time for the same amount of water to Journal of Textiles 7 0 Time

  13. Establishment of a robust hepatitis C virus replicon cell line over-expressing P-glycoprotein that facilitates analysis of P-gp drug transporter effects on inhibitor antiviral activity.

    Science.gov (United States)

    Hernandez, Dennis; Falk, Paul; Yu, Fei; Zhai, Guangzhi; Quan, Yong; Faria, Teresa; Cao, Kai; Scola, Paul; McPhee, Fiona

    2013-01-01

    P-glycoprotein (P-gp) is an active efflux pump affecting the pharmacokinetic (PK) profiles of drugs that are P-gp substrates. The Caco-2 bi-directional assay is widely used to identify drug-P-gp interactions in vitro. For molecules exhibiting non-classical drug properties however, ambiguous results limit its use in lead optimization. The goal of this study was to develop a robust cell-based assay system to directly measure the role of P-gp-driven efflux in reducing the potency of hepatitis C virus (HCV) replication inhibitors. Vinblastine (Vin) was employed to select for a Vin-resistant HCV replicon (313-11) from the parental cell line (377-2). The 313-11 cell line was >50-fold resistant to Vin and over-expressed P-gp, as determined by Western immunoblots. Increased expression of P-gp was mediated by up-regulation of the MDR1 transcript. The reduced potency of different classes of HCV replication inhibitors in the 313-11 P-gp cell line was restored in the presence of known P-gp inhibitors. Addition of the P-gp inhibitor, tariquidar, increased the uptake of a radiolabeled HCV replication inhibitor by 14-fold in the 313-11 replicon cell line. Finally, a positive correlation was demonstrated between potency in the 313-11 replicon and the bi-directional Caco-2 efflux ratio for a panel of HCV protease inhibitors. In conclusion, a robust P-gp HCV replicon cell-based assay has been developed to measure the effect of the P-gp efflux pump on the potency of different classes of HCV replication inhibitors. This system establishes a direct correlation between antiviral activity and the effect of P-gp efflux in a single cell line.

  14. Preface: 18th Aps-Sccm and 24th Airapt

    Science.gov (United States)

    Collins, Gilbert; Moore, David S.; Yoo, Choong-Shik; Buttler, William; Furlanetto, Michael; Evans, William

    2014-05-01

    The 18th Biennial International Conference of the APS Topical Group on Shock Compression of Condensed Matter in conjunction with the 24th Biennial International Conference of the International Association for the Advancement of High Pressure Science & Technology (AIRAPT) was held at the Westin Hotel in Seattle, Washington from 7-12 July, 2013. This is only the second time that these two organizations have held a Joint Conference — the first was 20 years previous (1993) in Colorado Springs, Colorado. Seattle was chosen for this joint conference because of its central location for the world-wide attendees as well as its metropolitan vibrancy. The scientific program consisted of 858 scheduled presentations organized into 23 topical areas and included contributed (537), invited (95), and plenary (6) lectures, as well as two poster sessions with 110 posters each. The scientific focus of the Joint Conference was on fundamental and applied research topics related to the static or dynamic compression of condensed matter. This multidisciplinary field of research encompasses areas of physics, chemistry, materials science, mechanics, geophysics and planetary physics, and applied mathematics. Experimental, computational and theoretical studies all play important roles. The organizers endeavored to intertwine static and dynamic experimental alongside computational and theoretical studies of similar materials in the organization of the sessions. This goal was aided by the addition of three special focus sessions on deep carbon budget, high energy density materials, and dynamic response of materials. 722 scientists and engineers from 25 countries registered at the conference, including 132 students from 12 countries. The attendee countries represented included: Argentina (2), Australia (2), Brazil (3), Canada (25), China (22), Czech Republic (2), France (35), Germany (19), India (6), Israel (21), Italy (10), Japan (49), Netherlands (1), Poland (1), Portugal (2), Russia (26

  15. Analysis of human reliability in the APS of fire. Application of NUREG-1921; Analisis de Fiabilidad Humana en el APS de Incendios. Aplicacion del NUREG-1921

    Energy Technology Data Exchange (ETDEWEB)

    Perez Torres, J. L.; Celaya Meler, M. A.

    2014-07-01

    An analysis of human reliability in a probabilistic safety analysis (APS) of fire aims to identify, describe, analyze and quantify, in a manner traceable, human actions that can affect the mitigation of an initiating event produced by a fire. (Author)

  16. Sediment Transport

    DEFF Research Database (Denmark)

    Liu, Zhou

    Flow and sediment transport are important in relation to several engineering topics, e.g. erosion around structures, backfilling of dredged channels and nearshore morphological change. The purpose of the present book is to describe both the basic hydrodynamics and the basic sediment transport...... mechanics. Chapter 1 deals with fundamentals in fluid mechanics with emphasis on bed shear stress by currents, while chapter 3 discusses wave boundary layer theory. They are both written with a view to sediment transport. Sediment transport in rivers, cross-shore and longshore are dealt with in chapters 2...

  17. Magnetic AP Stars in the Hertzsprung-Russell Diagram

    Science.gov (United States)

    Hubrig, S.; North, P.; Mathys, G.

    2000-08-01

    The evolutionary state of magnetic Ap stars is rediscussed using the recently released Hipparcos data. The distribution of the magnetic Ap stars of mass below 3 Msolar in the H-R diagram differs from that of the normal stars in the same temperature range at a high level of significance. Magnetic stars are concentrated toward the center of the main-sequence band. This is shown in two forms of the H-R diagram: one where logL is plotted against logTeff and a version more directly tied to the observed quantities, showing the astrometry-based luminosity (Arenou & Luri) against the (B2-G)0 index of Geneva photometry. In particular, it is found that magnetic fields appear only in stars that have already completed at least approximately 30% of their main-sequence lifetime. No clear picture emerges as to the possible evolution of the magnetic field across the main sequence. Hints of some (loose) relations between magnetic field strength and other stellar parameters are found: stars with shorter periods tend to have stronger fields, as do higher temperature and higher mass stars. A marginal trend of the magnetic flux to be lower in more slowly rotating stars may possibly be seen as suggesting a dynamo origin for the field. No correlation between the rotation period and the fraction of the main-sequence lifetime completed is observed, indicating that the slow rotation in these stars must already have been achieved before they became observably magnetic. Based on data from the ESA Hipparcos satellite and on observations collected at the European Southern Observatory (La Silla, Chile; ESO programs Nos. 43.7-004, 44.7-012, 49.7-030, 50.7-067, 51.7-041, 52.7-063, 53.7-028, 54.E-0416, and 55.E-0751), at the Observatoire de Haute-Provence (Saint-Michel l'Observatoire, France), at Kitt Peak National Observatory, and at the Canada-France-Hawaii Telescope.

  18. Radioactive air emissions notice of construction for installation and operation of a waste retrieval system and tanks 241-AP-102 and 241-AP-104 project

    Energy Technology Data Exchange (ETDEWEB)

    DEXTER, M.L.

    1999-11-15

    This document serves as a notice of construction (NOC) pursuant to the requirements of Washington Administrative Code (WAC) 246 247-060, and as a request for approval to modify pursuant to 40 Code of Federal Regulations (CFR) 61 07 for the installation and operation of one waste retrieval system in the 24 1 AP-102 Tank and one waste retrieval system in the 241 AP 104 Tank Pursuant to 40 CFR 61 09 (a)( 1) this application is also intended to provide anticipated initial start up notification Its is requested that EPA approval of this application will also constitute EPA acceptance of the initial start up notification Project W 211 Initial Tank Retrieval Systems (ITRS) is scoped to install a waste retrieval system in the following double-shell tanks 241-AP 102-AP 104 AN 102, AN 103, AN-104, AN 105, AY 102 AZ 102 and SY-102 between now and the year 2011. Because of the extended installation schedules and unknowns about specific activities/designs at each tank, it was decided to submit NOCs as that information became available This NOC covers the installation and operation of a waste retrieval system in tanks 241 AP-102 and 241 AP 104 Generally this includes removal of existing equipment installation of new equipment and construction of new ancillary equipment and buildings Tanks 241 AP 102 and 241 AP 104 will provide waste feed for immobilization into a low activity waste (LAW) product (i.e. glass logs) The total effective dose equivalent (TEDE) to the offsite maximally exposed individual (MEI) from the construction activities is 0 045 millirem per year The unabated TEDE to the offsite ME1 from operation of the mixer pumps is 0 042 millirem per year.

  19. Toxic epidermal necrolysis related to AP (pemetrexed plus cisplatin) and gefitinib combination therapy in a patient with metastatic non-small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    Ji-Jie Huang; Shu-Xiang Ma; Xue Hou; Zhao Wang; Yin-Duo Zeng; Tao Qin; Xiao-Xiao Dinglin; Li-Kun Chen

    2015-01-01

    Toxic epidermal necrolysis (TEN) is a rare acute life-threatening mucocutaneous disorder that is mostly drug-related (80%-95%). It is clinical y characterized as a widespread sloughing of the skin and mucosa. AP regimen (pemetrexed plus cisplatin) has been the preferred first-line chemotherapy for metastatic non-squamous non-small cell lung cancer (NSCLC). Gefitinib, a small-molecule epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), has already been recommended as a first-line treatment in EGFR-mutant metastatic NSCLC. We report rare presentation of TEN involving adverse effects of AP and gefitinib combination treatment in a 42-year-old woman diagnosed with metastatic NSCLC harboring an EGFR mutation. On the 21st day after administration of the first cycle of AP regimen and the 8th day after the initiation of gefitinib treatment, she developed an acne-like rash, oral ulcer, and conjunctivitis, which later became blisters and ultimately denuded. The characteristic clinical courses were decisive for the diagnosis of TEN. Treatment with systemic steroids and immunoglobulin as well as supportive treatment led to an improvement of her general condition and a remarkable recovery.

  20. Pseudoephedrine inhibits T-cell activation by targeting NF-κB, NFAT and AP-1 signaling pathways.

    Science.gov (United States)

    Fiebich, Bernd L; Collado, Juan A; Stratz, Cristian; Valina, Christian; Hochholzer, Willibald; Muñoz, Eduardo; Bellido, Luz M

    2012-02-01

    Pseudoephedrine (PSE) is a stereoisomer of ephedrine that is commonly used as a nasal decongestant in combination with other anti-inflammatory drugs for the symptomatic treatment of some common pathologies such as common cold. Herein, we describe for the first time the effects of PSE on T-cell activation events. We found that PSE inhibits interleukin-2 (IL-2) and tumor necrosis factor (TNF) alpha-gene transcription in stimulated Jurkat cells, a human T-cell leukemia cell line. To further characterize the inhibitory mechanisms of PSE at the transcriptional level, we examined the transcriptional activities of nuclear factor kappa B (NF-κB), nuclear factor of activated T cells (NFAT), and activator protein-1 (AP-1) transcription factors and found that PSE inhibited NF-κB-dependent transcriptional activity without affecting either the phosphorylation, the degradation of the cytoplasmic NF-κB inhibitory protein, IκBα or the DNA-binding activity. However, phosphorylation of the p65/RelA subunit was clearly inhibited by PSE in stimulated cells. In addition, PSE inhibited the transcriptional activity of NFAT without interfering with the calcium-induced NFAT dephosphorylation event, which represents the major signaling pathway for its activation. NFAT cooperates with c-Jun, a compound of the AP-1 complex, to activate target genes, and we also found that PSE inhibited both JNK activation and AP-1 transcriptional activity. These findings provide new mechanistic insights into the potential immunomodulatory activities of PSE and highlight their potential in designing novel therapeutic strategies to manage inflammatory diseases.

  1. A novel cell line derived from pleomorphic adenoma expresses MMP2, MMP9, TIMP1, TIMP2, and shows numeric chromosomal anomalies.

    Directory of Open Access Journals (Sweden)

    Aline Semblano Carreira Falcão

    Full Text Available Pleomorphic adenoma is the most common salivary gland neoplasm, and it can be locally invasive, despite its slow growth. This study aimed to establish a novel cell line (AP-1 derived from a human pleomorphic adenoma sample to better understand local invasiveness of this tumor. AP-1 cell line was characterized by cell growth analysis, expression of epithelial and myoepithelial markers by immunofluorescence, electron microscopy, 3D cell culture assays, cytogenetic features and transcriptomic study. Expression of matrix metalloproteinases (MMPs and their tissue inhibitors (TIMPs was also analyzed by immunofluorescence and zymography. Furthermore, epithelial and myoepithelial markers, MMPs and TIMPs were studied in the tumor that originated the cell line. AP-1 cells showed neoplastic epithelial and myoepithelial markers, such as cytokeratins, vimentin, S100 protein and smooth-muscle actin. These molecules were also found in vivo, in the tumor that originated the cell line. MMPs and TIMPs were observed in vivo and in AP-1 cells. Growth curve showed that AP-1 exhibited a doubling time of 3.342 days. AP-1 cells grown inside Matrigel recapitulated tumor architecture. Different numerical and structural chromosomal anomalies were visualized in cytogenetic analysis. Transcriptomic analysis addressed expression of 7 target genes (VIM, TIMP2, MMP2, MMP9, TIMP1, ACTA2 e PLAG1. Results were compared to transcriptomic profile of non-neoplastic salivary gland cells (HSG. Only MMP9 was not expressed in both libraries, and VIM was expressed solely in AP-1 library. The major difference regarding gene expression level between AP-1 and HSG samples occurred for MMP2. This gene was 184 times more expressed in AP-1 cells. Our findings suggest that AP-1 cell line could be a useful model for further studies on pleomorphic adenoma biology.

  2. A novel cell line derived from pleomorphic adenoma expresses MMP2, MMP9, TIMP1, TIMP2, and shows numeric chromosomal anomalies.

    Science.gov (United States)

    Falcão, Aline Semblano Carreira; Kataoka, Maria Sueli da Silva; Ribeiro, Nélson Antonio Bailão; Diniz, José Antonio Picanço; Alves, Sérgio Melo; Ribeiro, André L Ribeiro; de Siqueira, Adriane Sousa; da Silva, Artur Luiz; Ramos, Rommel Thiago Jucá; Freitas, Vanessa M; Jaeger, Ruy G; Pinheiro, João J V

    2014-01-01

    Pleomorphic adenoma is the most common salivary gland neoplasm, and it can be locally invasive, despite its slow growth. This study aimed to establish a novel cell line (AP-1) derived from a human pleomorphic adenoma sample to better understand local invasiveness of this tumor. AP-1 cell line was characterized by cell growth analysis, expression of epithelial and myoepithelial markers by immunofluorescence, electron microscopy, 3D cell culture assays, cytogenetic features and transcriptomic study. Expression of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) was also analyzed by immunofluorescence and zymography. Furthermore, epithelial and myoepithelial markers, MMPs and TIMPs were studied in the tumor that originated the cell line. AP-1 cells showed neoplastic epithelial and myoepithelial markers, such as cytokeratins, vimentin, S100 protein and smooth-muscle actin. These molecules were also found in vivo, in the tumor that originated the cell line. MMPs and TIMPs were observed in vivo and in AP-1 cells. Growth curve showed that AP-1 exhibited a doubling time of 3.342 days. AP-1 cells grown inside Matrigel recapitulated tumor architecture. Different numerical and structural chromosomal anomalies were visualized in cytogenetic analysis. Transcriptomic analysis addressed expression of 7 target genes (VIM, TIMP2, MMP2, MMP9, TIMP1, ACTA2 e PLAG1). Results were compared to transcriptomic profile of non-neoplastic salivary gland cells (HSG). Only MMP9 was not expressed in both libraries, and VIM was expressed solely in AP-1 library. The major difference regarding gene expression level between AP-1 and HSG samples occurred for MMP2. This gene was 184 times more expressed in AP-1 cells. Our findings suggest that AP-1 cell line could be a useful model for further studies on pleomorphic adenoma biology.

  3. TinyCoAP: A Novel Constrained Application Protocol (CoAP Implementation for Embedding RESTful Web Services in Wireless Sensor Networks Based on TinyOS

    Directory of Open Access Journals (Sweden)

    Anna Calveras

    2013-05-01

    Full Text Available In this paper we present the design and implementation of the Constrained Application Protocol (CoAP for TinyOS, which we refer to as TinyCoAP. CoAP seeks to apply the same application transfer paradigm and basic features of HTTP to constrained networks, while maintaining a simple design and low overhead. The design constraints of Wireless Sensor Networks (WSNs require special attention in the design process of the CoAP implementation. We argue that better performance and minimal resource consumption can be achieved developing a native library for the operating system embedded in the network. TinyOS already includes in its distribution an implementation of CoAP called CoapBlip. However, this is based on a library not originally designed to meet the requirements of TinyOS. We demonstrate the effectiveness of our approach by a comprehensive performance evaluation. In particular, we test and evaluate TinyCoAP and CoapBlip in a real scenario, as well as solutions based on HTTP. The evaluation is performed in terms of latency, memory occupation, and energy consumption. Furthermore, we evaluate the reliability of each solution by measuring the goodput obtained in a channel affected by Rayleigh fading. We also include a study on the effects that high workloads have on a server.

  4. Third-order TRANSPORT: A computer program for designing charged particle beam transport systems

    Energy Technology Data Exchange (ETDEWEB)

    Carey, D.C. [Fermi National Accelerator Lab., Batavia, IL (United States); Brown, K.L.; Rothacker, F. [Stanford Linear Accelerator Center, Menlo Park, CA (United States)

    1995-05-01

    TRANSPORT has been in existence in various evolutionary versions since 1963. The present version of TRANSPORT is a first-, second-, and third-order matrix multiplication computer program intended for the design of static-magnetic beam transport systems. This report discusses the following topics on TRANSPORT: Mathematical formulation of TRANSPORT; input format for TRANSPORT; summaries of TRANSPORT elements; preliminary specifications; description of the beam; physical elements; other transformations; assembling beam lines; operations; variation of parameters for fitting; and available constraints -- the FIT command.

  5. Performance evaluation of rotating pump jet mixing of radioactive wastes in Hanford Tanks 241-AP-102 and -104

    Energy Technology Data Exchange (ETDEWEB)

    Onishi, Y.; Recknagle, K.P.

    1998-07-01

    The purpose of this study was to confirm the adequacy of a single mixer pump to fully mix the wastes that will be stored in Tanks 241-AP-102 and -104. These Hanford double-shell tanks (DSTs) will be used as staging tanks to receive low-activity wastes from other Hanford storage tanks and, in turn, will supply the wastes to private waste vitrification facilities for eventual solidification. The TEMPEST computer code was applied to Tanks AP-102 and -104 to simulate waste mixing generated by the 60-ft/s rotating jets and to determine the effectiveness of the single rotating pump to mix the waste. TEMPEST simulates flow and mass/heat transport and chemical reactions (equilibrium and kinetic reactions) coupled together. Section 2 describes the pump jet mixing conditions the authors evaluated, the modeling cases, and their parameters. Section 3 reports model applications and assessment results. The summary and conclusions are presented in Section 4, and cited references are listed in Section 5.

  6. Scribble1/AP2 Complex Coordinates NMDA Receptor Endocytic Recycling

    Directory of Open Access Journals (Sweden)

    Nicolas H. Piguel

    2014-10-01

    Full Text Available The appropriate trafficking of glutamate receptors to synapses is crucial for basic synaptic function and synaptic plasticity. It is now accepted that NMDA receptors (NMDARs internalize and are recycled at the plasma membrane but also exchange between synaptic and extrasynaptic pools; these NMDAR properties are also key to governing synaptic plasticity. Scribble1 is a large PDZ protein required for synaptogenesis and synaptic plasticity. Herein, we show that the level of Scribble1 is regulated in an activity-dependent manner and that Scribble1 controls the number of NMDARs at the plasma membrane. Notably, Scribble1 prevents GluN2A subunits from undergoing lysosomal trafficking and degradation by increasing their recycling to the plasma membrane following NMDAR activation. Finally, we show that a specific YxxR motif on Scribble1 controls these mechanisms through a direct interaction with AP2. Altogether, our findings define a molecular mechanism to control the levels of synaptic NMDARs via Scribble1 complex signaling.

  7. Initial commissioning results from the APS loss monitor system

    Science.gov (United States)

    Patterson, Donald R.

    1997-01-01

    The design of the beam loss monitor system for the Argonne National Laboratory Advanced Photon Source is based on using a number of air dielectric coaxial cables as long ionization chambers. Results to date show that the loss monitor is useful in helping to determine the cause of injection losses and losses large enough to limit circulating currents in the storage ring to short lifetimes. Sensitivities ranging from 13 to 240 pC of charge collected in the injector BTS (booster-to-storage-ring) loss monitor per picocoulomb of loss have been measured, depending on the loss location. These results have been used to predict that the storage ring loss monitor leakage current limit of 10 pA per cable should allow detection of losses resulting in beam lifetimes of 100 hours or less with 100 mA stored beam. Significant DC bias levels associated with the presence of stored beam have been observed. These large bias levels are most likely caused by the loss monitor responding to hard x-ray synchrotron radiation. No such response to synchrotron radiation was observed during earlier tests at SSRL. However, the loss monitor response to average stored beam current in APS has provided a reasonable alternative to the DC current transformer (DCCT) for measuring beam lifetimes.

  8. Transport Phenomena.

    Science.gov (United States)

    McCready, Mark J.; Leighton, David T.

    1987-01-01

    Discusses the problems created in graduate chemical engineering programs when students enter with a wide diversity of understandings of transport phenomena. Describes a two-semester graduate transport course sequence at the University of Notre Dame which focuses on fluid mechanics and heat and mass transfer. (TW)

  9. SCF/C-Kit/JNK/AP-1 Signaling Pathway Promotes Claudin-3 Expression in Colonic Epithelium and Colorectal Carcinoma.

    Science.gov (United States)

    Wang, Yaxi; Sun, Tingyi; Sun, Haimei; Yang, Shu; Li, Dandan; Zhou, Deshan

    2017-04-06

    Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. In the present study, colorectal cancer (CRC) tissues, HT-29 and DLD-1 CRC cell lines, CRC murine model (C57BL/6 mice) and c-kit loss-of-function mutant mice were used. We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) inhibitors. Chromatin immunoprecipitation (ChIP) and luciferase reporter assay showed that SCF/c-kit signaling significantly promoted activator protein-1 (AP-1) binding with CLDN-3 promoter and enhanced its transcription activity. Furthermore, decreased expression of claudin-3 was obtained in the colonic epithelium from the c-Kit loss-of-function mutant mice. In conclusion, SCF/c-kit-JNK/AP-1 signaling pathway significantly promoted claudin-3 expression in colonic epithelium and CRC, which could contribute to epithelial barrier function maintenance and to CRC development.

  10. Reiterative AP2a activity controls sequential steps in the neural crest gene regulatory network.

    Science.gov (United States)

    de Crozé, Noémie; Maczkowiak, Frédérique; Monsoro-Burq, Anne H

    2011-01-04

    The neural crest (NC) emerges from combinatorial inductive events occurring within its progenitor domain, the neural border (NB). Several transcription factors act early at the NB, but the initiating molecular events remain elusive. Recent data from basal vertebrates suggest that ap2 might have been critical for NC emergence; however, the role of AP2 factors at the NB remains unclear. We show here that AP2a initiates NB patterning and is sufficient to elicit a NB-like pattern in neuralized ectoderm. In contrast, the other early regulators do not participate in ap2a initiation at the NB, but cooperate to further establish a robust NB pattern. The NC regulatory network uses a multistep cascade of secreted inducers and transcription factors, first at the NB and then within the NC progenitors. Here we report that AP2a acts at two distinct steps of this cascade. As the earliest known NB specifier, AP2a mediates Wnt signals to initiate the NB and activate pax3; as a NC specifier, AP2a regulates further NC development independent of and downstream of NB patterning. Our findings reconcile conflicting observations from various vertebrate organisms. AP2a provides a paradigm for the reiterated use of multifunctional molecules, thereby facilitating emergence of the NC in vertebrates.

  11. The History of the APS Shock Compression of Condensed Matter Topical Group

    Energy Technology Data Exchange (ETDEWEB)

    Forbes, J W

    2001-05-02

    In order to provide broader scientific recognition and to advance the science of shock compressed condensed matter, a group of American Physical Society (APS) members worked within the Society to make this field an active part of the APS. Individual papers were presented at APS meetings starting in the 1940's and shock wave sessions were organized starting with the 1967 Pasadena meeting. Shock wave topical conferences began in 1979 in Pullman, WA. Signatures were obtained on a petition in 1984 from a balanced cross-section of the shock wave community to form an APS Topical Group (TG). The APS Council officially accepted the formation of the Shock Compression of Condensed Matter (SCCM) TG at its October 1984 meeting. This action firmly aligned the shock wave field with a major physical science organization. Most early topical conferences were sanctioned by the APS while those held after 1992 were official APS meetings. The topical group organizes a shock wave topical conference in odd numbered years while participating in shock wave/high pressure sessions at APS general meetings in even numbered years.

  12. History of the APS Topical Group on Shock Compression of Condensed Matter

    Energy Technology Data Exchange (ETDEWEB)

    Forbes, J W

    2001-10-19

    In order to provide broader scientific recognition and to advance the science of shock compressed condensed matter, a group of American Physical Society (APS) members worked within the Society to make this field an active part of the APS. Individual papers were presented at APS meetings starting in the 1940's and shock wave sessions were organized starting with the 1967 Pasadena meeting. Shock wave topical conferences began in 1979 in Pullman, WA. Signatures were obtained on a petition in 1984 from a balanced cross-section of the shock wave community to form an APS Topical Group (TG). The APS Council officially accepted the formation of the Shock Compression of Condensed Matter (SCCM) TG at its October 1984 meeting. This action firmly aligned the shock wave field with a major physical science organization. Most early topical conferences were sanctioned by the APS while those held after 1992 were official APS meetings. The topical group organizes a shock wave topical conference in odd numbered years while participating in shock wavehigh pressure sessions at APS general meetings in even numbered years.

  13. The interaction between the adaptor protein APS and Enigma is involved in actin organisation

    DEFF Research Database (Denmark)

    Barres, Romain; Gonzalez, Teresa; Le Marchand-Brustel, Yannick

    2005-01-01

    APS (adaptor protein with PH and SH2 domains) is an adaptor protein phosphorylated by several tyrosine kinase receptors including the insulin receptor. To identify novel binding partners of APS, we performed yeast two-hybrid screening. We identified Enigma, a PDZ and LIM domain-containing protein...... that was previously shown to be associated with the actin cytoskeleton. In HEK 293 cells, Enigma interacted specifically with APS, but not with the APS-related protein SH2-B. This interaction required the NPTY motif of APS and the LIM domains of Enigma. In NIH-3T3 cells that express the insulin receptor, Enigma...... and APS were partially co-localised with F-actin in small ruffling structures. Insulin increased the complex formation between APS and Enigma and their co-localisation in large F-actin containing ruffles. While in NIH-3T3 and HeLa cells the co-expression of both Enigma and APS did not modify the actin...

  14. 75 FR 20774 - Establishment of Class E Airspace; Fort A.P. Hill, VA

    Science.gov (United States)

    2010-04-21

    ... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Fort A.P. Hill, VA... Register December 7, 2009 that establishes Class E airspace at Fort A.P. Hill, VA. DATES: Effective Date..., Eastern Service Center, Federal Aviation Administration, P.O. Box 20636, Atlanta, Georgia 30320;...

  15. Coming Soon: CADRE (Career Advancement and Development Resources and Education) website for all APS members

    Science.gov (United States)

    The Council of the American Phytopathological Society (APS) approved an initiative in February 2013 to create a web resource called CADRE (Career Advancement and Development Resources and Education). CADRE is to provide APS members an archive of articles, videos, and webinars about a variety of prof...

  16. Amphioxus and lamprey AP-2 genes: implications for neural crest evolution and migration patterns

    Science.gov (United States)

    Meulemans, Daniel; Bronner-Fraser, Marianne

    2002-01-01

    The neural crest is a uniquely vertebrate cell type present in the most basal vertebrates, but not in cephalochordates. We have studied differences in regulation of the neural crest marker AP-2 across two evolutionary transitions: invertebrate to vertebrate, and agnathan to gnathostome. Isolation and comparison of amphioxus, lamprey and axolotl AP-2 reveals its extensive expansion in the vertebrate dorsal neural tube and pharyngeal arches, implying co-option of AP-2 genes by neural crest cells early in vertebrate evolution. Expression in non-neural ectoderm is a conserved feature in amphioxus and vertebrates, suggesting an ancient role for AP-2 genes in this tissue. There is also common expression in subsets of ventrolateral neurons in the anterior neural tube, consistent with a primitive role in brain development. Comparison of AP-2 expression in axolotl and lamprey suggests an elaboration of cranial neural crest patterning in gnathostomes. However, migration of AP-2-expressing neural crest cells medial to the pharyngeal arch mesoderm appears to be a primitive feature retained in all vertebrates. Because AP-2 has essential roles in cranial neural crest differentiation and proliferation, the co-option of AP-2 by neural crest cells in the vertebrate lineage was a potentially crucial event in vertebrate evolution.

  17. Foundations for College and Beyond: Looking Back on AP Art History

    Science.gov (United States)

    Schoenbohm, Laurel

    2013-01-01

    It was years after this author's AP Art History course in high school, and two years after college. She and some friends decided to fill a day during the Thanksgiving visits appreciating fine art. Prior to that AP course her senior year of high school, touring an art museum had seemed like the equivalent of going to the dentist. But after…

  18. Understanding and Using the New Guided-Inquiry AP Chemistry Laboratory Manual

    Science.gov (United States)

    Cacciatore, Kristen L.

    2014-01-01

    To support teaching and learning in the advanced placement (AP) chemistry laboratory, the College Board published a laboratory manual, "AP Chemistry Guided-Inquiry Experiments: Applying the Science Practices," in 2013 as part of the redesigned course. This article provides a discussion of the rationale for the existence of the manual as…

  19. NACS: non-overlapping AP's caching scheme to reduce handoff in 802.11 wireless LAN

    CERN Document Server

    Tariq, Usman; Hong, Man-Pyo

    2011-01-01

    With the escalation of the IEEE 802.11 based wireless networks, voice over IP and analogous applications are also used over wireless networks. Recently, the wireless LAN systems are spaciously deployed for public Internet services. In public wireless LAN systems, reliable user authentication and mobility support are indispensable issues. When a mobile device budges out the range of one access point (AP) and endeavor to connect to new AP, it performs handoff. Contemporarily, PNC and SNC were proposed to propagate the MN context to the entire neighboring AP's on the wireless network with the help of neighbor graph. In this paper, we proposed a non-overlapping AP's caching scheme (NACS), which propagates the mobile node context to those AP's which do not overlap with the current AP. To capture the topology of non-overlapping AP's in the wireless network, non-overlapping graph (NOG) is generated at each AP. Simulation results shows that NACS reduces the signaling cost of propagating the MN context to the neighbor...

  20. 76 FR 78007 - Ocean Transportation Intermediary License; Revocation

    Science.gov (United States)

    2011-12-15

    ... Ocean Transportation Intermediary License; Revocation The Federal Maritime Commission hereby gives notice that the following Ocean Transportation Intermediary license has been revoked pursuant to section... corresponding date shown below: License Number: 018407N. Name: Pacific Atlantic Lines, Inc. Address:...

  1. [Effect of astragalus polysaccharide on sensitivity of leukemic cell line HL-60 to NK cell cytotoxicity and its mechanism].

    Science.gov (United States)

    Zeng, Peng-Yun; Deng, Li-Li; Yue, Ling-Ling; Zhang, Lian-Sheng

    2012-08-01

    The objective of this study was to explore the effect of astragalus polysaccharide (APS) on sensitivity of leukemic cell line HL-60 to NK cell cytotoxicity and its mechanism. The cytotoxicities of NK cells against HL-60 cells were analyzed by LDH releasing assay at different effect-to-target cell ratios (E:T) before and after treated with APS. The gene expression of MHC class I chain-related (MICA) in HL-60 cells before and after APS treatment was assayed with RT-PCR. Protein expression of MICA in HL-60 cells was assayed by flow cytometry before and after treated by APS. The results showed that after treated with APS 15 mg/ml for 48 h, the cytotoxicities of NK cells against HL-60 cells enhanced at different effect-to-target (P HL-60 cells were up-regulated (P HL-60 cells, thus enhance sensitivity of HL-60 cells to cytotoxicity of NK cells.

  2. Effect of External Photoevaporation on the Radial Transport of Volatiles and the Water Snowline in the Solar Nebula

    Science.gov (United States)

    Kalyaan, Anusha; Desch, Steven

    2017-01-01

    The Sun was likely born in a high mass star forming region [1]. Such a birth environment with a proximity to a nearby O or B star would photoevaporate the sun’s protoplanetary disk and cause an outward mass flow from the outer edge, as well as truncation of the disk, as seen in the Orion proplyds (although not as intensely)[2]. Photoevaporation likely explains the currently observed ~47 AU edge of the Kuiper Belt in our solar system [3], and more compellingly, the origin of certain short-lived radionuclides (such as Fe60), which cannot be successfully explained by a nebular origin [4][5]. Such a mass loss mechanism should affect the radial transport processes in the snowline region and along with temperature, has the potential to alter the location of the snowline.In this context, and in the light of recent ALMA observational results indicative of non-traditional behavior of snowlines and volatile transport in disks [6][7], this work studies what effect a photoevaporative mass loss from the outer disk may have on the volatile transport around the snowline region between ~1-10 AU in the disk. We build on the model of [8] and explore the effects of a steep photoevaporated non-uniform $\\alpha$ disk on radial transport of volatiles and small icy solids by incorporating the advection-diffusion equations as in [9] and condensation/evaporation of volatiles. We present results of these simulations, including volatile mass fluxes, ice/rock ratios, and snow line locations, in protoplanetary disks like the solar nebula.References: [1] Adams, F.C., 2010, ARAA 48,47 [2] Henney, W.J., & O’Dell, C.R., 1999, AJ, 118, 2350 [3] Trujillo,C.A. & Brown,M.E., 2001, ApJL,554,L95 [4] Hester, J.J., & Desch, S.J., 2005,ASPC, 341,107 [5] Wadhwa, M. et al. , 2007, Protostars & Planets V, 835 [5 [6] Cieza, L.A., et al., 2016, Nature,535,258 [7] Huang, J, et al. et al., 2016, ApJL, 823, L18 [8] Kalyaan, A., et al., 2015, ApJ, 815, 112 [9] Desch, S.J., et al., (in review).

  3. The Westinghouse AP1000 plant design: a generation III+ reactor with unique proven passive safety technology

    Energy Technology Data Exchange (ETDEWEB)

    Demetri, K. J.; Leipner, C. I.; Marshall, M. L., E-mail: demetrkj@westinghouse.com [Westinghouse Electric Company, 1000 Westinghouse Drive, Cranberry Township, PA 16066 (United States)

    2015-09-15

    The AP1000 plant is an 1100-M We pressurized water reactor with passive safety features and extensive plant simplifications and standardization that simplify construction, operation, maintenance, safety, and cost. The AP1000 plant is based on proven pressurized water reactor (PWR) technology, with an emphasis on safety features that rely solely on natural forces. These passive safety features are combined with simple, active, defense-in-depth systems used during normal plant operations which also provide the first level of defense against more probable events. This paper focuses on specific safety and licensing topics: the AP1000 plant robustness to be prepared for extreme events that may lead to catastrophic loss of infrastructure, such as the Fukushima Dai-ichi event, and the AP1000 plant compliance with the safety objectives for new plants. The first deployment of the AP1000 plant formally began in July 2007 when Westinghouse Electric Company and its consortium partner, the Shaw Group, signed contracts for four AP1000 units on coastal sites of Sanmen and Haiyang, China. Both sites have the planned ability to accommodate at least six AP1000 units; construction is largely concurrent for all four units. Additionally, the United States (U.S.) Nuclear Regulatory Commission (NRC) issued combined licenses (COLs) to allow Southern Nuclear Operating Company (SNC) and South Carolina Electric and Gas Company (SCE and G) to construct and operate AP1000 plants. Within this paper, the various factors that contribute to an unparalleled level of design, construction, delivery, and licensing certainty for any new AP1000 plant projects are described. These include: 1) How the AP1000 plant design development and reviews undertaken in the United States, China and Europe increase licensing certainty. 2) How the AP1000 passive plant robustness against extreme events that result in large loss of infrastructure further contributes to the licensing certainty in a post

  4. Transport Efficiency of Gas-jet Tape Transport System

    Institute of Scientific and Technical Information of China (English)

    WandXudong; MaRuichang; XieYuanxiang; XingYebin; XuShuwei; LiZhankui

    2003-01-01

    The Gas-jet Tape Transport System (GTTS) was used in several on-line experiments. The transport efficiency of GTTS was studied with three different inorganic salts, PbI2, PbC12 and NaC1, by using a 252 Cf source. The fission fragments of 252Cf were transported and collected by the GTTS before measurement. Nitrogen gas was used as a substitute of cxpensive Helium gas, in consideration of longer stopping range of fragments. The fragments were also directly collected on Aluminum foils with thickness of 50μm for original yield measurement.

  5. Glossary for the implementation of Health in All Policies (HiAP).

    Science.gov (United States)

    Freiler, Alix; Muntaner, Carles; Shankardass, Ketan; Mah, Catherine L; Molnar, Agnes; Renahy, Emilie; O'Campo, Patricia

    2013-12-01

    Health in All Policies (HiAP) is becoming increasingly popular as a governmental strategy to improve population health by coordinating action across health and non-health sectors. A variety of intersectoral initiatives may be used in HiAP that frame health determinants as the bridge between policies and health outcomes. The purpose of this glossary is to present concepts and terms useful in understanding the implementation of HiAP as a cross-sectoral policy. The concepts presented here were applied and elaborated over the course of case studies of HiAP in multiple jurisdictions, which used key informant interviews and the systematic review of literature to study the implementation of specific HiAP initiatives.

  6. New Protein Vector ApE1 for Targeted Delivery of Anticancer Drugs

    Directory of Open Access Journals (Sweden)

    N. V. Pozdniakova

    2012-01-01

    Full Text Available A new chimeric gene ApE1 encoding the receptor-binding domain of the human alpha-fetoprotein fused to a sequence of 22 glutamic acid residues was constructed. A new bacterial producer strain E. coli SHExT7 ApE1 was selected for ApE1 production in a soluble state. A simplified method was developed to purify ApE1 from bacterial biomass. It was shown that the new vector protein selectively interacts with AFP receptors on the tumor cell surface and can be efficiently accumulated in tumor cells. In addition, ApE1 was shown to be stable in storage and during its chemical modification. An increased number of carboxyl groups in the molecule allows the production of cytotoxic compound conjugates with higher drug-loading capacity and enhanced tumor targeting potential.

  7. Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench

    Directory of Open Access Journals (Sweden)

    Timotijević Gordana S.

    2010-01-01

    Full Text Available Aspartic proteinase gene (FeAP12 has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP characterized by the presence of a plant-specific insert (PSI, unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.

  8. 49 CFR 192.179 - Transmission line valves.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Transmission line valves. 192.179 Section 192.179... line valves. (a) Each transmission line, other than offshore segments, must have sectionalizing block valves spaced as follows, unless in a particular case the Administrator finds that alternative...

  9. 49 CFR 192.375 - Service lines: Plastic.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Service lines: Plastic. 192.375 Section 192.375... § 192.375 Service lines: Plastic. (a) Each plastic service line outside a building must be installed... terminate above ground level and outside the building, if— (i) The above ground level part of the...

  10. 14 CFR 23.993 - Fuel system lines and fittings.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Fuel system lines and fittings. 23.993 Section 23.993 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... Fuel System Components § 23.993 Fuel system lines and fittings. (a) Each fuel line must be...

  11. Transport in gyrokinetic tokamaks

    Energy Technology Data Exchange (ETDEWEB)

    Mynick, H.E.; Parker, S.E.

    1995-01-01

    A comprehensive study of transport in full-volume gyrokinetic (gk) simulations of ion temperature gradient driven turbulence in core tokamak plasmas is presented. Though this ``gyrokinetic tokamak`` is much simpler than experimental tokamaks, such simplicity is an asset, because a dependable nonlinear transport theory for such systems should be more attainable. Toward this end, we pursue two related lines of inquiry. (1) We study the scalings of gk tokamaks with respect to important system parameters. In contrast to real machines, the scalings of larger gk systems (a/{rho}{sub s} {approx_gt} 64) with minor radius, with current, and with a/{rho}{sub s} are roughly consistent with the approximate theoretical expectations for electrostatic turbulent transport which exist as yet. Smaller systems manifest quite different scalings, which aids in interpreting differing mass-scaling results in other work. (2) With the goal of developing a first-principles theory of gk transport, we use the gk data to infer the underlying transport physics. The data indicate that, of the many modes k present in the simulation, only a modest number (N{sub k} {approximately} 10) of k dominate the transport, and for each, only a handful (N{sub p} {approximately} 5) of couplings to other modes p appear to be significant, implying that the essential transport physics may be described by a far simpler system than would have been expected on the basis of earlier nonlinear theory alone. Part of this analysis is the inference of the coupling coefficients M{sub kpq} governing the nonlinear mode interactions, whose measurement from tokamak simulation data is presented here for the first time.

  12. Doppler imaging of chemical spots on magnetic Ap/Bp stars. Numerical tests and assessment of systematic errors

    Science.gov (United States)

    Kochukhov, O.

    2017-01-01

    Context. Doppler imaging (DI) is a powerful spectroscopic inversion technique that enables conversion of a line profile time series into a two-dimensional map of the stellar surface inhomogeneities. DI has been repeatedly applied to reconstruct chemical spot topologies of magnetic Ap/Bp stars with the goal of understanding variability of these objects and gaining an insight into the physical processes responsible for spot formation. Aims: In this paper we investigate the accuracy of chemical abundance DI and assess the impact of several different systematic errors on the reconstructed spot maps. Methods: We have simulated spectroscopic observational data for two different Fe spot distributions with a surface abundance contrast of 1.5 dex in the presence of a moderately strong dipolar magnetic field. We then reconstructed chemical maps using different sets of spectral lines and making different assumptions about line formation in the inversion calculations. Results: Our numerical experiments demonstrate that a modern DI code successfully recovers the input chemical spot distributions comprised of multiple circular spots at different latitudes or an element overabundance belt at the magnetic equator. For the optimal reconstruction based on half a dozen spectral intervals, the average reconstruction errors do not exceed 0.10 dex. The errors increase to about 0.15 dex when abundance distributions are recovered from a few and/or blended spectral lines. Ignoring a 2.5 kG dipolar magnetic field in chemical abundance DI leads to an average relative error of 0.2 dex and maximum errors of 0.3 dex. Similar errors are encountered if a DI inversion is carried out neglecting a non-uniform continuum brightness distribution and variation of the local atmospheric structure. None of the considered systematic effects lead to major spurious features in the recovered abundance maps. Conclusions: This series of numerical DI simulations proves that inversions based on one or two spectral

  13. Foreword: 18th Aps-Sccm and 24th Airapt

    Science.gov (United States)

    Collins, Gilbert; Moore, David S.; Yoo, Choong-Shik

    2014-05-01

    This second joint conference between the APS Topical Group on Shock Compression of Condensed Matter and the International Association for the Advancement of High Pressure Science and Technology (AIRAPT) demonstrates that static and dynamic compression of condensed matter continues to be a vibrant field of science and engineering. It is also by its nature an interdisciplinary field, incorporating chemistry, materials science, solid mechanics, plasma physics, and condensed matter physics, and utilizes theoretical, computational, and experimental tools. Recent years have brought about many advances in loading platforms, diagnostics, and computations that are leading to the emergence of many new avenues of research. These advances are also breathing new life into traditional topics such as equations of state, phase transformations, and chemistry at extreme conditions. The plenary lectures by Gennady Kanel, Karl Syassen, David Ceperley, Jon Eggert, Duck Young Kim, and Richard Kraus spanned the disciplines of static and dynamic high pressure physics and illustrated the breadth of the field. They also showed that interesting and important problems remain for researchers of the future to solve. The main guiding principal in the organization of this conference was to intertwine static and dynamical experimental alongside computational and theoretical studies of similar materials. To achieve this goal, we arranged the conference to include static, dynamic, and computational components in the same sessions, quite often taking presenters out of their comfort zone. The three special sessions on Deep Carbon Budget (organized by Giulia Galli and Rus Hemley), High Energy Density Materials (organized by Raymond Jeanloz and Jon Eggert), and Dynamic Response of Materials (organized by Yogendra Gupta and John Sarrao) furthered this guiding principal. We also endeavored to represent the breadth of static and dynamic high pressure science and technology, notably beyond that done at

  14. Renal effects of atriopeptin (AP) II in conscious SHR

    Energy Technology Data Exchange (ETDEWEB)

    Smits, J.F.; Debets, J.M.; Struyker-Boudier, H.A.; Daemen, M.J.

    1986-03-05

    Diuresis and natriuresis following APs has been suggested to depend upon increased renal blood flow (RBF). In a previous study, they found that APII infusions in conscious SHR decrease RBF. In this study, they report effects of APII on glomerular filtration rate (GFR) and renal excretory function in conscious SHR. Male SHR were equipped with arterial and venous catheters. Furthermore, a special catheter was implanted into the bladder to allow continuous urine sampling from conscious, freely moving rats. Animals were allowed at least 4 days to recover. In one group of SHR, APII (N=7) or saline (N=7) was infused to quantitate excretion of water, Na/sup +/, and K/sup +/. Two other groups of SHR (N=8 each) were infused with /sup 51/Cr-EDTA and /sup 125/I-PAH. GFR and renal plasma flow (ERPF) were calculated from urine and a mid-time plasma sample during APII or saline infusion. Following changes in infusion rates, urine from the first 5 min was discarded, after which a 10-min collection was made. APII (0.5-4 ..mu..g/kg/min) increased water, Na/sup +/ and K/sup +/ output maximally during 2 ..mu..g/kg/min. ERPF decreased from 10.7 +/- 1.1 ml/min to 7.9 +/- 0.5 ml/min. GFR did not change significantly. Filtration fraction increased from 24 +/- 1% to 33 +/- 2%. Fractional water excretion increased from 0.8 +/- 0.2% to 2.5 +/- 0.7%. Saline infusions did not affect renal function. The results indicate that in conscious SHR, APII causes diuresis and natriuresis which does not depend upon increased GFR, but, rather, upon a primary tubular effect.

  15. Hidrocefalia após meningite - relato de caso

    Directory of Open Access Journals (Sweden)

    Tássia Sabbadin Mancilha

    2015-10-01

    Full Text Available A malformação do sistema nervoso central é um dos fatores de risco para meningite no período neonatal, a qual se caracteriza pela ocorrência de um processo infeccioso nas meninges, ocorrendo entre o nascimento e o 28º dia de vida. As complicações mais frequentemente associadas á meningite neonatal são a ventriculite, síndrome convulsiva, síndrome da secreção impropria do hormônio antidiurético, hidrocefalia, abcesso cerebral, hemorragia intracraniana, leucomalácia e coleção subdural. O objetivo do presente relato é descrever um caso de um paciente portador de uma má formação do sistema nervoso central, que apresentou aos 28 dias de vida quadro de meningite bacteriana que evoluiu para uma hidrocefalia. Após abordagem cirúrgica para colocação de DVE, seguida de complicações e troca de DVP, paciente apresenta suspeita de hipertensão intracraniana, quadro de crises convulsivas e atraso no desenvolvimento neuropsicomotor. A meningite no período neonatal é considerada uma patologia grave, cuja mortalidade varia de 15 a 50%. Sua incidência varia de 0,3 a 1 caso por 1000 nascidos vivos. Dentre os sobreviventes cerca de 50 a 75% evoluem com deficiência auditiva, deficiência visual, atraso no desenvolvimento neuropsicomotor, retardo mental, convulsões e hidrocefalia. Desta forma fica evidente, devido ao diagnóstico clinico ser difícil, a necessidade de manejo e tratamento precoce a fim de minimizar o prognóstico e as dificuldades que poderá apresentar o paciente no futuro.

  16. AP-2α Inhibits c-MYC Induced Oxidative Stress and Apoptosis in HaCaT Human Keratinocytes

    Directory of Open Access Journals (Sweden)

    Lei Yu

    2009-01-01

    AP-2 may have a direct effect on the c-myc gene. Chromatin immunoprecipitation assays demonstrated that AP-2 proteins bound to a cluster of AP-2 binding sites located within a 2 kb upstream regulatory region of c-myc These results suggest that the negative regulation of AP-2 on c-MYC activity was achieved through binding of AP-2 protein to the c-myc gene. The effects of AP-2 on c-MYC induced ROS accumulation and apoptosis in epidermal keratinocytes are likely to play an important role in cell growth, differentiation and carcinogenesis of the skin.

  17. In situ gel-forming AP-57 peptide delivery system for cutaneous wound healing.

    Science.gov (United States)

    Li, Xiaoling; Fan, Rangrang; Tong, Aiping; Yang, Meijia; Deng, Jiaojiao; Zhou, Liangxue; Zhang, Xiaoning; Guo, Gang

    2015-11-10

    In situ gel-forming system as local drug delivery system in dermal traumas has generated a great interest. Accumulating evidence shows that antimicrobial peptides play pivotal roles in the process of wound healing. Here in this study, to explore the potential application of antimicrobial peptide in wound healing, biodegradable poly(L-lactic acid)-Pluronic L35-poly(L-lactic acid) (PLLA-L35-PLLA) was developed at first. Then based on this polymer, an injectable in situ gel-forming system composed of human antimicrobial peptides 57 (AP-57) loaded nanoparticles and thermosensitive hydrogel was prepared and applied for cutaneous wound healing. AP-57 peptides were enclosed with biocompatible nanoparticles (AP-57-NPs) with high drug loading and encapsulation efficiency. AP-57-NPs were further encapsulated in a thermosensitive hydrogel (AP-57-NPs-H) to facilitate its application in cutaneous wound repair. As a result, AP-57-NPs-H released AP-57 in an extended period and exhibited quite low cytotoxicity and high anti-oxidant activity in vitro. Moreover, AP-57-NPs-H was free-flowing liquid at room temperature, and can form non-flowing gel without any crosslink agent upon applied on the wounds. In vivo wound healing assay using full-thickness dermal defect model of SD rats indicated that AP-57-NPs-H could significantly promote wound healing. At day 14 after operation, AP-57-NPs-H treated group showed nearly complete wound closure of 96.78 ± 3.12%, whereas NS, NPs-H and AP-57-NPs group recovered by about 68.78 ± 4.93%, 81.96 ± 3.26% and 87.80 ± 4.62%, respectively. Histopathological examination suggested that AP-57-NPs-H could promote cutaneous wound healing through enhancing granulation tissue formation, increasing collagen deposition and promoting angiogenesis in the wound tissue. Therefore, AP-57-NPs-H might have potential application in wound healing.

  18. 76 FR 43374 - CSX Transportation, Inc.; Abandonment Exemption; in Hardin County, OH

    Science.gov (United States)

    2011-07-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Surface Transportation Board CSX Transportation, Inc.; Abandonment Exemption; in Hardin County, OH CSX... Street and Champion Court, in Kenton, Hardin County, Ohio. The line traverses United States...

  19. Analysis of AP 1000 ReactorPower Control System%AP1000反应堆控制系统特点分析

    Institute of Scientific and Technical Information of China (English)

    张小冬; 刘琳

    2011-01-01

    通过对核电机组常见的控制模式以及AP1000采用的控制模式的介绍,总结出各种模式的优缺点,并分析AP1000所采取的控制模式的先进性,对三门核电厂首台机组及后续机组的运行控制模式提出建议;还结合AP1000反应堆功率控制系统的特点,对在正常运行期间可能遇到的问题加以分析,并提出相应的对策.%The reactor power control modes applied by common nuclear power plants and AP1000 is introduced briefly. The advantages and shortcomings are compared and summarized. Advice is also made to the control strategy of the first and continued SMNPC plants. Additionally,the characteristics of the AP1000 reactor control system are summarized and problems which the operators will probably encounter are also analyzed.

  20. PfAP2Tel, harbouring a non-canonical DNA-binding AP2 domain, binds to Plasmodium falciparum telomeres.

    Science.gov (United States)

    Sierra-Miranda, Miguel; Vembar, Shruthi S; Delgadillo, Dulce María; Ávila-López, P A; Vargas, Miguel; Hernandez-Rivas, Rosaura

    2017-04-04

    The telomeres of the malaria parasite Plasmodium falciparum are essential not only for chromosome end maintenance during blood stage development in humans but also to generate genetic diversity by facilitating homologous recombination of subtelomeric, multigene virulence families such as var and rifin. However, other than the telomerase PfTERT, proteins that act at P. falciparum telomeres are poorly characterized. To isolate components that bind to telomeres, we performed oligonucleotide pulldowns and electromobility shift assays with a telomeric DNA probe and identified a non-canonical member of the ApiAP2 family of transcription factors, PfAP2Tel (encoded by PF3D7_0622900), as a component of the P. falciparum telomere-binding protein complex. PfAP2Tel is expressed throughout the intra-erythrocytic life cycle and localizes to the nuclear periphery, co-localizing with telomeric clusters. Furthermore, EMSAs using the recombinant protein demonstrated direct binding of PfAP2Tel to telomeric repeats in vitro, while genome-wide chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) corroborated the high specificity of this protein to telomeric ends of all 14 chromosomes in vivo. Taken together, our data describe a novel function for ApiAP2 proteins at chromosome ends and open new avenues to study the molecular machinery that regulates telomere function in P. falciparum.

  1. Transport Phenomena.

    Science.gov (United States)

    Shah, D. B.

    1984-01-01

    Describes a course designed to achieve a balance between exposing students to (1) advanced topics in transport phenomena, pointing out similarities and differences between three transfer processes and (2) common methods of solving differential equations. (JN)

  2. Nicaragua - Transportation

    Data.gov (United States)

    Millennium Challenge Corporation — The evaluation examines impacts of the Transportation Project in three ways. First, we calculate economic rates of return associated with reduced user costs for each...

  3. Medidas orofaciais em nipobrasileiros após crescimento puberal

    Directory of Open Access Journals (Sweden)

    Tassiana Barbeiro Fragoso de Sá

    2013-04-01

    Full Text Available OBJETIVO: descrever medidas orofaciais em nipobrasileiros após crescimento puberal e verificar diferenças entre medidas de mulheres negras, brancas e nipobrasileiras. MÉTODO: a casuística foi composta de 90 sujeitos japoneses ou descendentes de japoneses, sem histórico de miscigenação, ambos os sexos, entre 20 e 50 anos, sem queixas ou atendimentos fonoaudiológicos atuais ou prévios em motricidade orofacial, sem tratamento ortodôntico atual e sem alteração cognitiva. As medidas avaliadas foram: lábio superior, lábio inferior, filtro, terço superior da face, terço médio da face, terço inferior da face, lados da face, abertura máxima de boca e abertura máxima de boca com a língua na papila. O instrumento utilizado foi o paquímetro eletrônico digital da marca Jomarca. Os dados foram comparados com os achados na literatura referentes às mulheres negras e brancas. RESULTADOS: os dados coletados mostraram homogeneidade entre as medidas; os valores para todas as medidas foram maiores nos homens, com relevância estatística positiva. As diferenças mais significantes entre as negras, brancas e nipobrasileiras foram: terço superior e filtro das nipobrasileiras menor do que de negras e brancas. Para o terço médio não houve diferença estatística significante. O lábio superior das nipobrasileiras é maior do que das brancas e o lábio inferior das nipobrasileiras menor do que das negras. Lados da face sem diferenças estatísticas. CONCLUSÃO: foi possível verificar que as médias das medidas foram sempre maiores nos homens, que houve relações significantes entre algumas medidas orofaciais e que houve diferença estatisticamente significante entre as estruturas das brancas, negras e nipobrasileiras.

  4. Understanding Slag Freeze Linings

    Science.gov (United States)

    Fallah-Mehrjardi, Ata; Hayes, Peter C.; Jak, Evgueni

    2014-09-01

    Slag freeze linings, the formation of protective deposit layers on the inner walls of furnaces and reactors, are increasingly used in industrial pyrometallurgical processes to ensure that furnace integrity is maintained in these aggressive, high-temperature environments. Most previous studies of freeze-linings have analyzed the formation of slag deposits based solely on heat transfer considerations. These thermal models have assumed that the interface between the stationary frozen layer and the agitated molten bath at steady-state deposit thickness consists of the primary phase, which stays in contact with the bulk liquid at the liquidus temperature. Recent experimental studies, however, have clearly demonstrated that the temperature of the deposit/liquid bath interface can be lower than the liquidus temperature of the bulk liquid. A conceptual framework has been proposed to explain the observations and the factors influencing the microstructure and the temperature of the interface at steady-state conditions. The observations are consistent with a dynamic steady state that is a balance between (I) the rate of nucleation and growth of solids on detached crystals in a subliquidus layer as this fluid material moves toward the stagnant deposit interface and (II) the dissolution of these detached crystals as they are transported away from the interface by turbulent eddies. It is argued that the assumption that the interface temperature is the liquidus of the bulk material represents only a limiting condition, and that the interface temperature can be between T liquidus and T solidus depending on the process conditions and bath chemistry. These findings have implications for the modeling approach and boundary conditions required to accurately describe these systems. They also indicate the opportunity to integrate considerations of heat and mass flows with the selection of melt chemistries in the design of future high temperature industrial reactors.

  5. Technical Steps to Support Nuclear Arsenal Downsizing: A Report by the APS Panel on Public Affairs

    Science.gov (United States)

    2010-02-01

    Technical Steps to Support Nuclear Arsenal Downsizing A RepoRt by the ApS pAnel on public AffAiRS TM Report Documentation Page Form ApprovedOMB No...00-2010 to 00-00-2010 4. TITLE AND SUBTITLE Technical Steps to Support Nuclear Arsenal Downsizing : A Report by the APS Panel on Public Affairs 5a...Contents Executive Summary 2 Introduction 3 Verifying Downsizing and Dismantlement 5 Sustaining Capability and Expertise 15 Ensuring Peaceful Uses of

  6. Glossary for the implementation of Health in All Policies (HiAP)

    OpenAIRE

    Freiler, Alix; Muntaner, Carles; Shankardass, Ketan; Mah, Catherine L.; Molnar, Agnes; Renahy, Emilie; O'Campo, Patricia

    2013-01-01

    Health in All Policies (HiAP) is becoming increasingly popular as a governmental strategy to improve population health by coordinating action across health and non-health sectors. A variety of intersectoral initiatives may be used in HiAP that frame health determinants as the bridge between policies and health outcomes. The purpose of this glossary is to present concepts and terms useful in understanding the implementation of HiAP as a cross-sectoral policy. The concepts presented here were a...

  7. Osiguravanje međuoperabilnosti za Internet stvari: iskustvo s testiranjem CoAP protokola

    OpenAIRE

    Chen, Nanxing; Viho, César; Baire, Anthony; Huang, Xiaohong; Zha, Jiexi

    2014-01-01

    Constrained Application Protocol (CoAP) je specijalizirani prijenosni protokol, dizajniran za realizaciju međuoperabilnosti uz ograničene mrežame i čvorove za primjene poput pametne energije, automatizacije u zgradarstvu itd. Kao važan i sveprisutan protokol za Internet stvari CoAP bi mogao biti implementiran kod velikog broja pametnih uređaja kako bi se ostvarile kooperativne usluge. Zbog toga je od velike važnosti postic´i visoku razinu međuoperabilnosti CoAP implementacija. U tom kontekstu...

  8. CoCoA+: an advanced congestion control mechanism for CoAP

    OpenAIRE

    Betzler, August; Gómez Montenegro, Carlos; Seyfettin Demirkol, Ilker; Paradells Aspas, Josep

    2015-01-01

    The Constrained Application Protocol (CoAP) has been designed by the Internet Engineering Task Force (IETF) for Internet of Things (IoT) devices. Due to the limited radio channel capacities and hardware resources of such devices, congestion can be a serious problem. CoAP addresses this important issue with a basic congestion control mechanism. CoCoA, an Internet-Draft proposal, introduced alternative congestion control mechanisms for CoAP. Yet, there has been limited evaluation of these conge...

  9. Panel Discussion : Report of the APS Ad-Hoc Committee on LGBT Issues

    Science.gov (United States)

    Atherton, Tim; Barthelemy, Ramon; Garmon, Savannah; Reeves, Kyle; APS Ad-Hoc Committee on LGBT Issues Team

    Following the presentation of the findings and recommendations of the APS Ad-Hoc Committee on LGBT Issues (C-LGBT) by Committee Chair Michael Falk, a panel discussion will be held featuring several members of the committee. The discussion will focus on how APS can best ensure the recommendations of the committee are carried out in a timely fashion and other ideas on future APS efforts toward LGBT inclusion in physics. Discussion topics will also include the research and other input that shaped the committee's findings and recommendations.

  10. On the structure of AP-4 responsive element in the LTR of Jembrana disease virus

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Previous studies with deletion and sequence analysis of JDV LTR showed that there is a putative AP-4 responsive element in LTR. By antisense transient assay and gel shifting assay, we for the first time demonstrated that AP-4 modulated JDV gene expression by binding DNA directly to bovine cells. The results, derived from site-directed mutagenesis experiments, suggest that the six base pairs of AP-4 binding site (CAGCTG) have different effects on JDV gene expression. When the first two base pairs changed to GC, JDV gene expression is severely decreased.

  11. 241-AP Tank Farm Construction Extent of Condition Review for Tank Integrity

    Energy Technology Data Exchange (ETDEWEB)

    Barnes, Travis J.; Gunter, Jason R.; Reeploeg, Gretchen E.

    2014-04-04

    This report provides the results of an extent of condition construction history review for the 241-AP tank farm. The construction history of the 241-AP tank farm has been reviewed to identify issues similar to those experienced during tank AY-102 construction. Those issues and others impacting integrity are discussed based on information found in available construction records, using tank AY-102 as the comparison benchmark. In the 241-AP tank farm, the sixth double-shell tank farm constructed, tank bottom flatness, refractory material quality, post-weld stress relieving, and primary tank bottom weld rejection were improved.

  12. Overexpression of transcription factor AP-2 stimulates the PA promoter of the human uracil-DNA glycosylase (UNG) gene through a mechanism involving derepression

    DEFF Research Database (Denmark)

    Aas, Per Arne; Pena Diaz, Javier; Liabakk, Nina Beate;

    2009-01-01

    within the region of DNA marked by PA. Footprinting analysis and electrophoretic mobility shift assays of PA and putative AP-2 binding regions with HeLa cell nuclear extract and recombinant AP-2alpha protein indicate that AP-2 transcription factors are central in the regulated expression of UNG2 m...... an inhibitory effect of endogenous AP-2 or AP-2-like factors....

  13. 49 CFR 192.369 - Service lines: Connections to cast iron or ductile iron mains.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Service lines: Connections to cast iron or ductile...) PIPELINE SAFETY TRANSPORTATION OF NATURAL AND OTHER GAS BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Customer Meters, Service Regulators, and Service Lines § 192.369 Service lines: Connections to cast iron...

  14. Central line infections - hospitals

    Science.gov (United States)

    ... infection; Central venous catheter - infection; CVC - infection; Central venous device - infection; Infection control - central line infection; Nosocomial infection - central line infection; Hospital acquired ...

  15. Slurry transport medium

    Energy Technology Data Exchange (ETDEWEB)

    Rosenthal, W.; Schiffman, L.

    1980-06-03

    This invention provides for an improvement in slurry transport systems, especially coal slurry lines. Instead of the usual use of fresh water resources which, in some geographic areas, are scarce for slurry transport, concentrated brine is used which is prepared from abundant salt water resources. Because of the higher density of this concentrated brine, it is a superior carrier of pulverized material. It diminishes the separation and settling tendency of slurry components during transport and particularly during shutdown. Other advantages in the use of concentrated brine include: freezing point depression which permits ease of transport during winter and at lower temperatures; dust suppression of stored coal; avoidance of spontaneous combustion of stored coal; inhibit freeze packing of dewatered pipeline coal; and diminished extent of corrosion in ferrous metal pipelines as compared to that which might occur with lower concentration brines. Important in the economy of the process is that the concentrated brine can be recycled. An inexpensive method for producing the concentrated brine is given.

  16. Ap( Bn,dV)上具有BT符号的Toeplitz算子%Toeplitz operators on Ap (Bn, dV) with symbol in BT

    Institute of Scientific and Technical Information of China (English)

    何莉; 曹广福

    2011-01-01

    研究了高维Bergman空间Ap(Bn,dV)(1 <p<∞)上具有BT符号的Toeplitz算子,利用Toeplitz算子的Berezin变换讨论了Toeplitz算子的有界性,得到了Ap(Bn,dV)上具有BT符号的Toeplitz算子的范数和本性范数的估计,推广了MIAO和ZHENG在Bergman空间LP(D,dA)上对具有BT符号的Toeplitz算子的范数和本性范数进行估计的结论.

  17. The null result of a search for pulsational variations of the surface magnetic field in the roAp star gamma Equulei

    CERN Document Server

    Kochukhov, O P; Landstreet, J D; Weiss, W W

    2004-01-01

    We describe an analysis of the time-resolved measurements of the surface magnetic field in the roAp star gamma Equ. We have obtained a high-resolution and high S/N spectroscopic time-series, and the magnetic field was determined using Zeeman resolved profiles of the Fe II 6149.25 A and Fe I 6173.34 A lines. Contrary to recent reports we do not find any evidence of magnetic variability with pulsation phase, and derive an upper limit of 5-10 G for pulsational modulation of the surface magnetic field in gamma Equ.

  18. Optimizing sales areas of combined transport chains

    Directory of Open Access Journals (Sweden)

    Philip Michalk

    2013-12-01

    Full Text Available Background: Combined transport chains (such as intermodal transport, have certain advantages. The main advantage from customer points of view is the possibility to bundle freight and thereby decrease transport costs. On the other hand, a combined transport chain can cause longer transport times, due to the necessary transshipment processes. Methods: The area around a terminal, in which a combined service has favourable properties to a customer in comparison to a direct transport, can be understood as a sales-area, in which a combined transport product is marketable. The aim of this paper was to find a method to determine the best shape and size of this area. Results and conclusions: The paper at hand lined out a method in order to calculate such a sales area and determine which geographical points around a terminal have an advantage in comparison to a direct transport service.

  19. Inflorescence meristem identity in rice is specified by overlapping functions of three AP1/FUL-like MADS box genes and PAP2, a SEPALLATA MADS box gene.

    Science.gov (United States)

    Kobayashi, Kaoru; Yasuno, Naoko; Sato, Yutaka; Yoda, Masahiro; Yamazaki, Ryo; Kimizu, Mayumi; Yoshida, Hitoshi; Nagamura, Yoshiaki; Kyozuka, Junko

    2012-05-01

    In plants, the transition to reproductive growth is of particular importance for successful seed production. Transformation of the shoot apical meristem (SAM) to the inflorescence meristem (IM) is the crucial first step in this transition. Using laser microdissection and microarrays, we found that expression of PANICLE PHYTOMER2 (PAP2) and three APETALA1 (AP1)/FRUITFULL (FUL)-like genes (MADS14, MADS15, and MADS18) is induced in the SAM during meristem phase transition in rice (Oryza sativa). PAP2 is a MADS box gene belonging to a grass-specific subclade of the SEPALLATA subfamily. Suppression of these three AP1/FUL-like genes by RNA interference caused a slight delay in reproductive transition. Further depletion of PAP2 function from these triple knockdown plants inhibited the transition of the meristem to the IM. In the quadruple knockdown lines, the meristem continued to generate leaves, rather than becoming an IM. Consequently, multiple shoots were formed instead of an inflorescence. PAP2 physically interacts with MAD14 and MADS15 in vivo. Furthermore, the precocious flowering phenotype caused by the overexpression of Hd3a, a rice florigen gene, was weakened in pap2-1 mutants. Based on these results, we propose that PAP2 and the three AP1/FUL-like genes coordinately act in the meristem to specify the identity of the IM downstream of the florigen signal.

  20. A phosphorylation defective retinoic acid receptor mutant mimics the effects of retinoic acid on EGFR mediated AP-1 expression and cancer cell proliferation

    Directory of Open Access Journals (Sweden)

    Kim Randie

    2002-10-01

    Full Text Available Abstract Background The effects of the vitamin A metabolite retinoic acid (RA are mediated at the transcriptional level by retinoic acid receptors (RAR. These proteins are part of a superfamily of transcription factors which activate target gene expression when bound to their respective ligands. In addition to ligand binding, heterodimerization with transcriptional cofactors and posttranslational modification such as phosphorylation are also critical for transactivation function. Previous studies have shown that phosphorylation of a serine residue at amino acid 77 in the RARα amino terminus was required for basal activation function of the transcription factor. Results We have determined that RA inhibits cyclin H and cdk7 expression thereby decreasing levels of phosphorylated RARα in human cancer cell lines. To determine the effects of decreased RARα phosphorylation in human cancer cells, we stably transfected a phosphorylation defective mutant RARα expression construct into SCC25 cultures. Cells expressing the mutant RARα proliferated more slowly than control clones. This decreased proliferation was associated with increased cyclin dependent kinase inhibitor expression and decreased S phase entry. In the absence of ligand, the RARα mutant inhibited AP-1 activity to an extent similar to that of RA treated control clones. Levels of some AP-1 proteins were inhibited due to decreased EGFR expression upstream in the signaling pathway. Conclusions These results indicate that hypophosphorylated RARα can mimic the anti-AP-1 effects of RA in the absence of ligand.

  1. Providing an efficient intelligent transportation system through detection, tracking and recognition of the region of interest in traffic signs by using non-linear SVM classifier in line with histogram oriented gradient and Kalman filter approach

    Indian Academy of Sciences (India)

    A H Mazinan; M Sarikhani

    2014-02-01

    With a focus on new researches in the area of intelligent transportation systems (ITS), an efficient approach has been investigated here. Based on the present view point, analysis of traffic signs are first considered via intelligence based approach, which is carried out through three main stages including detection, tracking and recognition, respectively, in this research. The key role of detection is to identify traffic signs by classification of road sign shapes in accordance with their signatures. This classification consists of four different shapes of circle, semicircle, triangle and square, as well. The linear classification of traffic sign is also carried out via support vector machine (SVM) by using one against all (OAA), since the present SVMs classifiers realized via linear kernel. The next step is to track traffic sign. It should be noted that this technique is now developed to reduce the searching mode in case of the whole area to be optimized its computational processing, consequently. This research work is investigated by realizing Kalman filter approach, where, finally, in recognition step, a feature of the region of interest (ROI) has been extracted for SVM classification. Histogram of oriented gradient (HOG) is realized in organizing the approach, as long as Gaussian kernel is also developed for non-linear SVM classifier.

  2. FFAG Beam Line for nuPIL - Neutrinos from PIon Beam Line

    Energy Technology Data Exchange (ETDEWEB)

    Lagrange, Jean-Baptiste [Fermilab; Pasternak, Jaroslaw [Rutherford; Bross, Alan [Fermilab; Liu, Ao [Fermilab; Appleby, Robert [Cockcroft Inst. Accel. Sci. Tech.; Tygier, Sam [Cockcroft Inst. Accel. Sci. Tech.

    2016-06-01

    The Long Baseline Neutrino Facilities (LBNF) program aims to deliver a neutrino beam for the Deep Underground Neutrino Experiment (DUNE). The current baseline for LBNF is a conventional magnetic horn and decay pipe system. Neutrinos from PIon beam Line (nuPIL) is a part of the optimization effort to optimize the LBNF. It consists of a pion beam line after the horn to clean the beam of high energy protons and wrong-sign pions before transporting them into a decay beam line, where instrumentation could be implemented. This paper focuses on the FFAG solution for this pion beam line. The resulting neutrino flux is also presented.

  3. La netnografía como herramienta de investigación en contextos on-line: una aplicación al análisis de la imagen de los servicios públicos de transporte

    Directory of Open Access Journals (Sweden)

    Agustí Casas-Romeo

    2014-02-01

    Full Text Available La netnografía es una nueva metodología de investigación cualitativa que adapta técnicas de investigación etnográficas al estudio de culturas y comunidades emergentes a través de la comunicación mediada por ordenador, y surge como respuesta al fenómeno cultural del crecimiento de Internet y al creciente número de consumidores que utilizan la Red para mantenerse informados y para crear relaciones y comunidades con otros consumidores que tienen similares estilos de vida y que consumen productos y/o servicios similares. Si bien, desde finales de los noventa la netnografía está tomando mayor protagonismo, se hace necesario un mayor número de trabajos que desarrollen aspectos relacionados con esta metodología; especialmente, en el contexto de la literatura de marketing, donde existen pocos trabajos centrados en la netnografía como herramienta de investigación comercial. Dada la escasez de trabajos, este estudio pretende analizar a nivel teórico la utilidad de la netnografía como herramienta de investigación en contextos on-line. Adicionalmente, se ilustra dicha utilidad a partir de un estudio desarrollado para el análisis de la imagen del servicio de autobuses de la ciudad de Madrid.

  4. Newly Identified Rydberg Emission Lines in Novae

    Science.gov (United States)

    Lynch, David K.; Rudy, R. J.; Bernstein, L. S.

    2008-09-01

    Newly Identified Rydberg Emission Lines in Novae David K. Lynch, Richard. J. Rudy (The Aerospace Corporation) & Lawrence S. Bernstein (Spectral Sciences, Inc.) Novae spectra in the near infrared frequently show a set of six emission lines that have not been positively identified (Williams, Longmore, & Geballe 1996, MNRAS, 279, 804; Lynch et al. 2001, AJ, 122, 2013; Rudy et al. 2002 ApJ, 573, 794; Lynch et al. 2004 Astron. J. 127, 1089-1097). These lines are at 0.8926, 1.1114, 1.1901, 1.5545, 2.0996 and 2.425 µm ± 0.005 µm. Krautter et al. (1984 A&A 137, 304) suggested that three of the lines were due to rydberg (hydrogenic) transitions in an unspecified atomic species that was in the 4th or 5th ionization stage (core charge = 4 & 5). We believe that Krautter et al.'s explanation is correct based on 4 additional lines that we have identified in the visible and near infrared spectrum of V723 Cassiopeiae. The observed Rydberg lines appear to originate from high angular momentum states with negligible quantum defects. The species cannot be determined with any certainty because in rydberg states, the outer electron sees a nucleus shielded by the inner electrons and together the inner atom appears to have a charge of +1, like hydrogen. As a result, the atom looks hydrogenic and species such as CV, NV, OV, MgV, SiV, etc. have their rydberg transitions at very similar wavelengths. All the lines represent permitted transitions, most likely formed by recombination. Atoms with core charges 4, 5 & 6 are rarely seen in the astrophysical environment because an extremely hot radiation field is necessary to ionize them. Thermonuclear runaways on the surface of a white dwarf can reach millions of degrees K, and thus there are enough X-ray photons available to achieve the necessary high ionization levels.

  5. The prediction of AE, ap, and Dst at time lags between 0 and 30 hours

    Science.gov (United States)

    Smart, D. F.; Garrett, H. B.; Shea, M. A.

    1979-01-01

    The magnetic activity indexes AE, ap, and Dst are correlated with approximately 35,000 hours of interplanetary plasma and magnetic field measurements acquired near the Earth (assembled by NASA/NSSDC into a composite data sat). Lag times between the indexes and solar wind parameters ranged from 0 to 30 hours. Correlations at lags less than 6 hours yield results in agreement with previous studies. At greater lags, the correlation coefficients between the solar wind parameters and AE and ap approach these parameters' autocorrelation (persistence) values. For Dst the correlation with solar wind parameters is lower than that with AE and ap in the 3 to 4 hour lag range whereas the autocorrelation of Dst is significantly higher over the entire 0 to 30 hour lag range. The implications of these differences between AE, ap, and Dst are discussed in terms of persistence of solar wind structure.

  6. Mapping of the molecular determinants involved in the interaction between eps15 and AP-2

    DEFF Research Database (Denmark)

    Iannolo, G; Salcini, A E; Gaidarov, I

    1997-01-01

    domain, located in its NH2-terminal region. In addition, a proline-rich region, located in the COOH-terminal portion of eps15, can bind to the Src homology 3 domain of the crk proto-oncogene product in vitro. Recently, coimmunoprecipitation between eps15 and AP-2, a major component of coated pits......, was reported. Here, we characterize the molecular determinants of the eps15/AP-2 interaction. The AP-2 binding region of eps15 is localized in its COOH-terminal region and spans approximately 80 amino acids. At least three molecular determinants, located at residues 650-660, 680-690, and 720-730, are involved...... in the binding. AP-2 binds to eps15 through its alpha subunit (alpha-adaptin); in particular, the COOH-terminal region of alpha-adaptin, the so-called alpha-ear, contains the eps15 binding region....

  7. Effects of podocin transfection on CD2AP distribution in HEK293 cells

    Institute of Scientific and Technical Information of China (English)

    Yugen SHA; Songming HUANG; Aihua ZHANG; Fei ZHAO; Ronghua CHEN

    2008-01-01

    The aim of this paper is to construct a podocin fluorescence expression vector and observe the effects of podocin transfection on CD2AP distribution in HEK293 cells. The pGEMT-easy vector containing the full-length cDNA encoding human podocin was cloned and digested with BamHI and XhoI. The digested full-length podocin was subcloned into pEGFP-C2. The constructed plas-mids were transfected into HEK293 cells and its effects on CD2AP distribution were observed by immunofluo-rescence. The pEGFP-NPHS2 expression vector was successfully constructed and podocin exclusively located on the HEK293 cell membrane. After podocin transfec-tion, CD2AP redistributed from the perinucleus to the cytoplasm in HEK293 cells. It can be concluded that podocin can recruit CD2AP to redistribute from the perinucleus to the cytoplasm in HEK293 cells.

  8. Karakterisasi ekstrak kasar fitase termofilik dari bakteri kawah Ijen Banyuwangi, isolat AP-17

    Directory of Open Access Journals (Sweden)

    Aline Puspita Kusumadjaja

    2010-12-01

    Full Text Available Crude thermophilic phytase was produced by isolate AP-17 that has been isolated from Ijen Crater Banyuwangi. Based on Gram test, isolate AP-17 was gram positive spore forming rod shape bacteria so that it was identified as Bacillus sp. AP-17. Crude thermophilic phytase isolated from Bacillus sp. AP-17 had the optimum temperature at 75 ° C with activity of 0.1413 U/ml, and its optimum pH was at pH 6 with activity of 0.0875 U/ml. The enzyme was stable when heated at 75 ° C for three hours and still had 90% activity when it was exposed at pH 5 €“8, optimum temperature, for one hour.

  9. An Analysis of Media’s Role: Case Study of Army Public School (APS Peshawar Attack

    Directory of Open Access Journals (Sweden)

    Qureshi Rameesha

    2016-12-01

    Full Text Available The study aimed at analyzing the role of media during and after terrorist attacks by examining the media handling of APS Peshawar attack. The sample consisted of males and females selected on convenience basis from universities of Rawalpindi and Islamabad. It was hypothesized that (1 Extensive media coverage of terrorist attacks leads to greater publicity/recognition of terrorist groups (2 Media coverage of APS Peshawar attack increased fear and anxiety in public (3 Positive media handling/coverage of APS Peshawar attack led to public solidarity and peace. The results indicate that i Media coverage of terrorist attacks does help terrorist groups to gain publicity and recognition amongst public ii Media coverage of Aps Peshawar attack did not increase fear/anxiety in fact it directed the Pakistani nation towards public solidarity and peace.

  10. Determination of Heats of Fusion: Using Differential Scanning Calorimetry for the AP Chemistry Courses.

    Science.gov (United States)

    Temme, Susan M.

    1995-01-01

    Describes an exercise designed to be used in an Advanced Placement (AP) chemistry course to accompany the study of thermodynamics. Uses Differential Scanning Calorimetry in teaching the concepts of thermochemistry and thermodynamics. (JRH)

  11. A self consistent chemically stratified atmosphere model for the roAp star 10 Aquilae

    CERN Document Server

    Nesvacil, Nicole; Ryabchikova, Tanya A; Kochukhov, Oleg; Akberov, Artur; Weiss, Werner W

    2012-01-01

    Context: Chemically peculiar A type (Ap) stars are a subgroup of the CP2 stars which exhibit anomalous overabundances of numerous elements, e.g. Fe, Cr, Sr and rare earth elements. The pulsating subgroup of the Ap stars, the roAp stars, present ideal laboratories to observe and model pulsational signatures as well as the interplay of the pulsations with strong magnetic fields and vertical abundance gradients. Aims: Based on high resolution spectroscopic observations and observed stellar energy distributions we construct a self consistent model atmosphere, that accounts for modulations of the temperature-pressure structure caused by vertical abundance gradients, for the roAp star 10 Aquilae (HD 176232). We demonstrate that such an analysis can be used to determine precisely the fundamental atmospheric parameters required for pulsation modelling. Methods: Average abundances were derived for 56 species. For Mg, Si, Ca, Cr, Fe, Co, Sr, Pr, and Nd vertical stratification profiles were empirically derived using the...

  12. 76 FR 73720 - Knowledge and Abilities Catalog for Nuclear Power Plant Operators: Westinghouse AP1000...

    Science.gov (United States)

    2011-11-29

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Knowledge and Abilities Catalog for Nuclear Power Plant Operators: Westinghouse AP1000 Pressurized..., NUREG-2103, Revision 0, ``Knowledge and Abilities Catalog for Nuclear Power Plant...

  13. Functions and Application of the AP2/ERF Transcription Factor Family in Crop Improvement

    Institute of Scientific and Technical Information of China (English)

    Zhao-Shi Xu; Ming Chen; Lian-Cheng Li; You-Zhi Ma

    2011-01-01

    Plants have acquired sophisticated stress response systems to adapt to changing environments. It is important to understand plants' stress response mechanisms in the effort to improve crop productivity under stressful conditions. The AP2/ERF transcription factors are known to regulate diverse processes of plant development and stress responses.In this study, the molecular characteristics and biological functions of AP2/ERFs in a variety of plant species were analyzed. AP2/ERFs,especially those in DREB and ERF subfamilies, are ideal candidates for crop improvement because their overexpression enhances tolerances to drought, salt, freezing, as well as resistances to multiple diseases in the transgenic plants. The comprehensive analysis of physiological functions is useful in elucidating the biological roles of AP2/ERF family genes in gene interaction, pathway regulation, and defense response under stress environments, which should provide new opportunities for the crop tolerance engineering.

  14. Microwave line of sight link engineering

    CERN Document Server

    Angueira, Pablo

    2012-01-01

    A comprehensive guide to the design, implementation, and operation of line of sight microwave link systems The microwave Line of Sight (LOS) transport network of any cellular operator requires at least as much planning effort as the cellular infrastructure itself. The knowledge behind this design has been kept private by most companies and has not been easy to find. Microwave Line of Sight Link Engineering solves this dilemma. It provides the latest revisions to ITU reports and recommendations, which are not only key to successful design but have changed dramatically in

  15. The Language of Lines.

    Science.gov (United States)

    Breig-Allen, Cheryl; Hill, Janet; Geismar-Ryan, Lori; Cadwell, Louise Boyd

    1998-01-01

    Describes a project about lines in the environment used with 2- and 3-year olds and based on the Reggio Emilia approach. Activities included making tracks with riding toys, drawing lines on papers, seeing cloud lines, and making lines with yarn and Cuisenaire rods. Shows how young children's observations and ongoing discoveries can uncover their…

  16. The Last Line Effect

    NARCIS (Netherlands)

    Beller, M.; Zaidman, A.E.; Karpov, A.

    2015-01-01

    Micro-clones are tiny duplicated pieces of code; they typically comprise only a few statements or lines. In this paper, we expose the “last line effect,” the phenomenon that the last line or statement in a micro-clone is much more likely to contain an error than the previous lines or statements. We

  17. Chemogenomic identification of Ref-1/AP-1 as a therapeutic target for asthma

    OpenAIRE

    Nguyen, Cu; Teo, Jia-Ling; Matsuda, Akihisa; Eguchi, Masakatsu; Emil Y Chi; William R Henderson; Kahn, Michael

    2003-01-01

    Asthma is characterized by an oxidant/antioxidant imbalance in the lungs leading to activation of redox-sensitive transcription factors, nuclear factor κB (NF-κB), and activator protein-1 (AP-1). To develop therapeutic strategies for asthma, we used a chemogenomics approach to screen for small molecule inhibitor(s) of AP-1 transcription. We developed a β-strand mimetic template that acts as a reversible inhibitor (pseudosubstrate) of redox proteins. This template incorporates an enedione moie...

  18. Antiphospholipid syndrome (APS) revisited: Would migraine headaches be included in future classification criteria?

    Science.gov (United States)

    Noureldine, Mohammad Hassan A; Haydar, Ali A; Berjawi, Ahmad; Elnawar, Rody; Sweid, Ahmad; Khamashta, Munther A; Hughes, Graham R V; Uthman, Imad

    2016-07-16

    Headaches have been extensively reported in Antiphospholipid syndrome (APS)/Antiphospholipid antibodies (aPL)-positive patients. The aim of this study was to highlight the prevalence of headaches among APS/aPL-positive patients and discuss its association with laboratory, clinical and imaging findings. We searched the literature through Google Scholar and PubMed for publications on the epidemiology, pathogenesis, laboratory, imaging and clinical findings, and management of headaches in APS/aPL-positive patients. The following keywords were used: Antiphospholipid, Hughes syndrome, anticardiolipin, lupus anticoagulant, anti-β2 glycoprotein I, headache, migraine, tension, and cluster. All reports published between 1969 and 2015 were included. Migraine is the most commonly reported type of headache in APS/aPL-positive patients. Thrombotic and platelet dysfunction hypotheses have been studied to uncover the pathogenic role of aPL in the development of headaches. Several studies are reporting higher levels of aPL in primary and secondary APS migraineurs, but only few reached statistical significance. Migraine patients without clinical signs/symptoms of cerebral infarction rarely show positive imaging findings. Digital subtraction angiography shows promise in demonstrating small vascular lesions otherwise not detected on computed tomography, magnetic resonance imaging, or cerebral angiograms. Although it may be solitary and harmless in many cases, the deleterious effect of migraine on the quality of life of APS patients prompts rapid diagnosis and proper management. An anticoagulation trial is advisable in APS patients with migraine as many cases of severe, refractory migraine resolved with anticoagulation therapy. The profile of migraine headaches discussed in this study permits its candidacy for inclusion in future APS classification criteria.

  19. Structural Determinants of DNA Binding by a P. falciparum ApiAP2 Transcriptional Regulator

    Energy Technology Data Exchange (ETDEWEB)

    Lindner, Scott E.; De Silva, Erandi K.; Keck, James L.; Llinás, Manuel (Princeton); (UW-MED)

    2010-11-05

    Putative transcription factors have only recently been identified in the Plasmodium spp., with the major family of regulators comprising the Apicomplexan Apetala2 (AP2) proteins. To better understand the DNA-binding mechanisms of these transcriptional regulators, we characterized the structure and in vitro function of an AP2 DNA-binding domain from a prototypical Apicomplexan AP2 protein, PF14{_}0633 from Plasmodium falciparum. The X-ray crystal structure of the PF14{_}0633 AP2 domain bound to DNA reveals a {beta}-sheet fold that binds the DNA major groove through base-specific and backbone contacts; a prominent {alpha}-helix supports the {beta}-sheet structure. Substitution of predicted DNA-binding residues with alanine weakened or eliminated DNA binding in solution. In contrast to plant AP2 domains, the PF14{_}0633 AP2 domain dimerizes upon binding to DNA through a domain-swapping mechanism in which the {alpha}-helices of the AP2 domains pack against the {beta}-sheets of the dimer mates. DNA-induced dimerization of PF14{_}0633 may be important for tethering two distal DNA loci together in the nucleus and/or for inducing functional rearrangements of its domains to facilitate transcriptional regulation. Consistent with a multisite binding mode, at least two copies of the consensus sequence recognized by PF14{_}0633 are present upstream of a previously identified group of sporozoite-stage genes. Taken together, these findings illustrate how Plasmodium has adapted the AP2 DNA-binding domain for genome-wide transcriptional regulation.

  20. Catalysis of Nanometer α-Fe2O3 on the Thermal Decomposition of AP

    Institute of Scientific and Technical Information of China (English)

    YANG Yi; LI Feng-sheng; LIU Hong-ying; JIANG Wei; CUI Ping

    2005-01-01

    Nanometer α-Fe2O3 catalysts were prepared by hydrolyzation in high temperature. Three kinds of precipitators, NaOH, (NH4)2CO3 and urea were used to compare the effect in the process of hydrolyzation. Nanometer sizer, transmission electron microscopy (TEM) and X-ray diffraction (XRD) were employed to test the profiles and diameters of the product particles. The test results indicate that the production is nanometer α-Fe2O3 with narrow particle size distribution (PSD) and good dispersibility. The catalysts are mixed with ammonia perchlorate (AP) in 1.0 wt.%. And the composite particles of catalysts with AP are prepared using a new solvent-nonsolvent method. Differential thermal analyzer (DTA) is employed to analysis the thermal decomposition of the composite particles and pure AP sample. The results imply that the thermal decomposition curve peaks of the samples in which nanometer α-Fe2O3 catalysts are added appear comparatively more ahead than that of pure AP sample. Among these mixtures added nanometer material, the smaller the particle diameter of catalyst is, the more ahead the thermal decomposition curve peaks of AP appear. The high and low temperature thermal decomposition curve peaks of AP mixed with the catalyst deposed by urea are more ahead of 77.8℃ and 9.7℃ than that of pure AP, respectively. The mechanism of the catalyst deposed by urea with smaller diameter and the distinct catalysis of the particles on the thermal decomposition of AP are discussed.