WorldWideScience

Sample records for apple metabolite phloretin

  1. Controlling transgene expression in subcutaneous implants using a skin lotion containing the apple metabolite phloretin

    OpenAIRE

    Gitzinger, Marc; Kemmer, Christian; El-Baba, Marie Daoud; Weber, Wilfried; Fussenegger, Martin

    2009-01-01

    Adjustable control of therapeutic transgenes in engineered cell implants after transdermal and topical delivery of nontoxic trigger molecules would increase convenience, patient compliance, and elimination of hepatic first-pass effect in future therapies. Pseudomonas putida DOT-T1E has evolved the flavonoid-triggered TtgR operon, which controls expression of a multisubstrate-specific efflux pump (TtgABC) to resist plant-derived defense metabolites in its rhizosphere habitat. Taking advantage ...

  2. Same ammo, different weapons: enzymatic extracts from two apple genotypes with contrasted susceptibilities to fire blight (Erwinia amylovora) differentially convert phloridzin and phloretin in vitro.

    Science.gov (United States)

    Gaucher, Matthieu; Dugé de Bernonville, Thomas; Guyot, Sylvain; Dat, James F; Brisset, Marie-Noëlle

    2013-11-01

    The necrogenic bacterium Erwinia amylovora responsible for the fire blight disease causes cell death in apple tissues to enrich intercellular spaces with nutrients. Apple leaves contain large amounts of dihydrochalcones (DHCs), including phloridzin and its aglycone phloretin. Previous work showed an important decrease in the constitutive DHCs stock in infected leaves, probably caused by transformation reactions during the infection process. At least two flavonoid transformation pathways have been described so far: deglucosylation and oxidation. The aim of the present study was to determine whether DHCs are differentially converted in two apple genotypes displaying contrasted susceptibilities to the disease. Different analyses were performed: i) enzymatic activity assays in infected leaves, ii) identification/quantification of end-products obtained after in vitro enzymatic reactions with DHCs, iii) evaluation of the bactericidal activity of end-products. The results of the enzymatic assays showed that deglucosylation was dominant over oxidation in the susceptible genotype MM106 while the opposite was observed in the resistant genotype Evereste. These data were confirmed by LC-UV/Vis-MS analysis of in vitro reaction mixtures, especially because higher levels of o-quinoid oxidation products of phloretin were measured by using the enzymatic extracts of Evereste infected leaves. Their presence correlated well with a strong bactericidal activity of the reaction mixtures. Thus, our results suggest that a differential transformation of DHCs occur in apple genotypes with a potential involvement in the establishment of the susceptibility or the resistance to fire blight, through the release of glucose or of highly bactericidal compounds respectively. PMID:23561298

  3. Hepatotoxicity and endothelial dysfunction induced by high choline diet and the protective effects of phloretin in mice.

    Science.gov (United States)

    Ren, Daoyuan; Liu, Yafei; Zhao, Yan; Yang, Xingbin

    2016-08-01

    The involvement of choline and its metabolite trimethylamine-N-oxide (TMAO) in endothelial dysfunction and atherosclerosis has been repeatedly confirmed. Phloretin, a dihydrochalcone flavonoid usually present in apples, possesses a variety of biological activities including vascular nutrition. This study was designed to investigate whether phloretin could alleviate or prevent high choline-induced vascular endothelial dysfunction and liver injury in mice. Mice were provided with 3% high choline water and given phloretin orally daily for 10 weeks. The high choline-treated mice showed the significant dyslipidemia and hyperglycemia with the impaired liver and vascular endothelium (p < 0.01). Administration of phloretin at 200 and 400 mg/kg bw significantly reduced the choline-induced elevation of serum TC, TG, LDL-C, AST, ALT, ET-1 and TXA2 (p < 0.01), and markedly antagonized the choline-induced decrease of serum PGI2, HDL-C and NO levels. Furthermore, phloretin elevated hepatic SOD and GSH-Px activities and decreased hepatic MDA levels of the mice exposed to high choline water. Moreover, histopathological test with the H&E and Oil Red O staining of liver sections confirmed the high choline diet-caused liver steatosis and the hepatoprotective effect of phloretin. These findings suggest that high choline causes oxidative damage, and phloretin alleviate vascular endothelial dysfunction and liver injury. PMID:27316781

  4. Enzymatic Synthesis of Novel Phloretin Glucosides

    OpenAIRE

    Pandey, Ramesh Prasad; Li, Tai Feng; Kim, Eun-Hee; Yamaguchi, Tokutaro; Park, Yong Il; Kim, Joong Su; Sohng, Jae Kyung

    2013-01-01

    A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli.

  5. Consumption of both low and high (-)-epicatechin apple puree attenuates platelet reactivity and increases plasma concentrations of nitric oxide metabolites: A randomized controlled trial

    NARCIS (Netherlands)

    Gasper, A.; Hollands, W.; Casgrain, A.; Saha, S.; Teucher, B.; Dainty, J.R.; Venema, D.P.; Hollman, P.C.H.

    2014-01-01

    We hypothesised that consumption of flavanol-containing apple puree would modulate platelet activity and increase nitric oxide metabolite status, and that high flavanol apple puree would exert a greater effect than low flavanol apple puree. 25 subjects consumed 230 g of apple puree containing 25 and

  6. Inhibition of Oxidation of Aqueous Emulsions of Omega-3 Fatty Acids and Fish Oil by Phloretin and Phloridzin

    Directory of Open Access Journals (Sweden)

    Afsana Yasmin

    2010-01-01

    Full Text Available The antioxidant properties of two apple dihydrochalcones, namely phloretin and phloridzin, were evaluated and compared with those of α-tocopherol and butylated hydroxytoluene (BHT. The effects were studied in an oil-in-water emulsion system containing methyl linolenate (ML, methyl eicosapentaenoate (MEPA, and methyl docosahexaenoate (MDHA in which oxidation was initiated by the peroxyl radical generator 2,2-azobis(2-amidinopropane dihydrochloride (AAPH and in fish oil where oxidation was initiated thermally. In the emulsion system, phloretin (1 and 5 mM completely inhibited the oxidation of ML tested as evidenced by the thiobarbituric acid reactive substances (TBARS assay. Under the same conditions, phloridzin was less effective than phloretin, but still more effective than α-tocopherol. Both phloretin and phloridzin molecules had a marginal inhibitory effect against oxidation of fish oil induced by heating at 70 °C for 3 hours, when compared to BHT. These results indicate that phloretin and phloridzin have the potential to suppress lipid oxidation in polyunsaturated fatty acid (PUFA containing foods.

  7. Apple phenolics and their contribution to enzymatic browning reactions

    Directory of Open Access Journals (Sweden)

    Wiesław Oleszek

    2014-02-01

    Full Text Available Chlorogenic acid, epicatechin, procyanidin B2 and C1 were isolated from apple skin. These compounds as well as quercetine and phloretine glycosides isolated from apples were studied individually and as mixtures for their participation in the enzymatic browning reactions. The importance of quercetine glycosides and the synergistic effect of phloridzin and phloretine xyloglucoside with chlorogenic acid and flavans in the browning reaction are reported.

  8. Consumption of both low and high (-)-epicatechin apple puree attenuates platelet reactivity and increases plasma concentrations of nitric oxide metabolites: a randomized controlled trial.

    Science.gov (United States)

    Gasper, Amy; Hollands, Wendy; Casgrain, Amelie; Saha, Shikha; Teucher, Birgit; Dainty, Jack R; Venema, Dini P; Hollman, Peter C; Rein, Maarit J; Nelson, Rebecca; Williamson, Gary; Kroon, Paul A

    2014-10-01

    We hypothesised that consumption of flavanol-containing apple puree would modulate platelet activity and increase nitric oxide metabolite status, and that high flavanol apple puree would exert a greater effect than low flavanol apple puree. 25 subjects consumed 230 g of apple puree containing 25 and 100mg epicatechin (low and high flavanol apple puree, respectively) and aspirin (75 mg) in random order. Measurements were made at baseline, acutely after treatment (2, 6 and 24 h), and after 14 d of treatment. Low flavanol apple puree significantly attenuated ADP and epinephrine-induced integrin-β3 expression 2 h and 6 h after consumption and ADP and epinephrine-induced P-selectin expression within 2h of consumption. High flavanol apple puree attenuated epinephrine and ADP-induced integrin-β3 expression after 2 and 6h. ADP and epinephrine-induced integrin-β3 expression was significantly attenuated 2, 6 and 24 h after consumption of aspirin, whilst 14 d aspirin consumption attenuated collagen-induced P-selectin expression only. The plasma total nitric oxide metabolite conc. was significantly increased 6h after consumption of both low and high flavanol apple purees. In conclusion, consumption of apple purees containing ⩾25 or 100 mg flavanols transiently attenuated ex vivo integrin-β3 and P-selectin expression and increased plasma nitric oxide metabolite conc. in healthy subjects, but the effect was not enhanced for the high flavanol apple puree. PMID:24929184

  9. Analysis of Fusarium avenaceum Metabolites Produced during Wet Apple Core Rot

    DEFF Research Database (Denmark)

    Sørensen, Jens Laurids; Phipps, Richard Kerry; Nielsen, Kristian Fog;

    2009-01-01

    Wet apple core rot (wACR) is a well-known disease of susceptible apple cultivars such as Gloster, Jona Gold, and Fuji. Investigations in apple orchards in Slovenia identified Fusarium avenaceum, a known producer of several mycotoxins, as the predominant causal agent of this disease. A LC-MS/MS me......Wet apple core rot (wACR) is a well-known disease of susceptible apple cultivars such as Gloster, Jona Gold, and Fuji. Investigations in apple orchards in Slovenia identified Fusarium avenaceum, a known producer of several mycotoxins, as the predominant causal agent of this disease. A LC...

  10. Liquid chromatography tandem mass spectrometry identification of apple polyphenol metabolites in human urine and plasma

    OpenAIRE

    Gallant, Vicki Ann

    2010-01-01

    Apples are a major dietary source of polyphenols in the Western diet and contain procyanidins, hydroxycinnamic acids, flavanols, dihydrochalcones and flavonols. Despite their abundance and familiarity very little research into their metabolism has been performed; research is required to elucidate the metabolic products of these polyphenols and characterise their absorption and excretion pathways. A human intervention study was designed specifically to investigate the absorption, metabolism, e...

  11. Direct evidence of plant- pathogenic activity of fungal metabolites of Trichothecium roseum on apple

    Czech Academy of Sciences Publication Activity Database

    Soukupová, Julie

    2006-01-01

    Roč. 1, č. 162 (2006), s. 65-68. ISSN 0301-486X R&D Projects: GA ČR GA203/04/0799; GA MŠk(CZ) LN00A141; GA MŠk(CZ) LC545 Institutional research plan: CEZ:AV0Z60870520 Keywords : metabolites Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 0.915, year: 2006

  12. Direct evidence of plant-pathogenic activity of fungal metabolites of Trichothecium roseum on apple

    Czech Academy of Sciences Publication Activity Database

    Žabka, Martin; Drastichová, K.; Jegorov, A.; Soukupová, Julie; Nedbal, Ladislav

    2006-01-01

    Roč. 162, č. 1 (2006), s. 65-68. ISSN 0301-486X R&D Projects: GA ČR GA203/04/0799; GA MŠk LN00A141; GA MŠk LC545 Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z60870520 Keywords : fluorescence * plant-pathogenic activity * fungal metabolites Subject RIV: EE - Microbiology, Virology Impact factor: 0.915, year: 2006

  13. Real-time PCR quantification of the AM-toxin gene and HPLC qualification of toxigenic metabolites from Alternaria species from apples.

    Science.gov (United States)

    Andersen, Birgitte; Smedsgaard, Jørn; Jørring, Ida; Skouboe, Pernille; Pedersen, Lars Hagsholm

    2006-09-01

    Some Alternaria species are able to produce plant pathogenic as well as toxic metabolites. In both agriculture and the food industry it is important know if toxigenic Alternaria are present to rapidly employ the correct corrective actions. The purpose of this work was to establish a real-time PCR method, which can detect and quantify apple pathogenic and toxigenic Alternaria. An AM-toxin I primer set, which could recognize Alternaria DNA only, was designed by using primers complementary to the AM-toxin I gene. The method could detect small amounts of DNA (4 pg) and still obtain a large dynamic range (4 decades) without interference from apple material. Eight Alternaria isolates were analyzed for the presence of AM-toxin I gene and their production of secondary metabolites. Then analyses showed that all eight isolates contained the AM toxin gene and were able to produce the plant pathogenic tentoxin in addition to AM toxin I. The analyses also showed the production of tenuazonic acid, alternariols, Altenuene, altenusin and/or altertoxin I in pure culture. Analyses of inoculated apples showed that both the AM-toxin gene and alternariol monomethyl ether could be detected. Morphological analyses suggested that the eight Alternaria strains, though they all carried the AM toxin genes, probably belong to different but closely related un-described Alternaria taxa in the A. tenuissima species-group based on morphological and chemical differences. PMID:16890318

  14. Active urea transport by the skin of Bufo viridis: Amiloride- and phloretin-sensitive transport sites

    International Nuclear Information System (INIS)

    Urea is actively transported inwardly (Ji) across the skin of the green toad Bufo viridis. Ji is markedly enhanced in toads adapted to hypertonic saline. The authors studied urea transport across the skin of Bufo viridis under a variety of experimental conditions, including treatment with amiloride and phloretin, agents that inhibit urea permeability in the bladder of Bufo marinus. Amiloride (10-4 M) significantly inhibited Ji in both adapted and unadapted animals and was unaffected by removal of sodium from the external medium. Phloretin (10-4 M) significantly inhibited Ji in adapted animals by 23-46%; there was also a reduction in Ji in unadapted toads at 10-4 and 5 x 10-4 M phloretin. A dose-response study revealed that the concentration of phloretin causing half-maximal inhibition (K1/2) was 5 x 10-4 M for adapted animals. Ji was unaffected by the substitution of sucrose for Ringer solution or by ouabain. They conclude (1) the process of adaptation appears to involve an increase in the number of amiloride- and phloretin-inhibitable urea transport sites in the skin, with a possible increase in the affinity of the sites for phloretin; (2) the adapted skin resembles the Bufo marinus urinary bladder with respect to amiloride and phloretin-inhibitable sites; (3) they confirm earlier observations that Ji is independent of sodium transport

  15. Genetical metabolomics in apples (Malus x domestica Borkh)

    OpenAIRE

    Khan, S. A.

    2012-01-01

    The aim of this thesis was finding genes that control the production of potentially health beneficial metabolites in apple fruits. The approach was genetic mapping of secondary metabolites such as phenolic compounds in an F1 progeny, leading to the detection of genetic loci that controlled these metabolites. At these genetic loci candidate genes were identified, using the whole genome sequence of apple, and it was investigated whether the expression of these candidate genes in the F1 progeny ...

  16. Newton's Apple

    Science.gov (United States)

    Hendry, Archibald W.

    2007-01-01

    Isaac Newton may have seen an apple fall, but it was Robert Hooke who had a better idea of where it would land. No one really knows whether or not Isaac Newton actually saw an apple fall in his garden. Supposedly it took place in 1666, but it was a tale he told in his old age more than 60 years later, a time when his memory was failing and his…

  17. Phenylpropanoid metabolites and expression of key genes involved in anthocyanin biosynthesis in the shaded peel of apple fruit in response to sun exposure.

    Science.gov (United States)

    Feng, Fengjuan; Li, Mingjun; Ma, Fengwang; Cheng, Lailiang

    2013-08-01

    The shaded peel of 'Fortune' (a red cultivar) and 'Mutsu' (a yellow/green cultivar) apple (Malus domestica Borkh.) was exposed to full sun by turning fruit 180° at about one week before harvest to determine the expression of key genes involved in anthocyanin synthesis in response to sunlight exposure and their relationships with the levels of anthocyanins and other phenolics. For the unturned (control) fruit, the shaded peel had lower expression levels of MdMYB10 (a transcriptional factor in the regulation of anthocyanin biosynthesis) and seven structural genes in anthocyanin synthesis (MdPAL, MdCHS, MdCHI, MdF3H, MdDFR1, MdLDOX, and MdUFGT), and lower levels of anthocyanins and flavonols than the sun-exposed peel in both cultivars. Exposure of the shaded peel to full sun caused marked up-regulation of the expression of MdMYB10 and all seven structural genes, which peaked between 6 h and 30 h after fruit turning, consequently leading to higher levels of anthocyanins, flavonols, and total phenolics than in the shaded peel and even in the sun-exposed peel of control fruit. Interestingly, the levels of flavonols were higher in the shaded peel of turned fruit (the original sun-exposed peel) than in the sun-exposed peel of both control and turned fruit in both cultivars, suggesting that competition for substrates exists in different branches of the phenylpropanoid pathway. These results indicate that sunlight exposure stimulates the expression of MdMYB10 and structural genes in anthocyanin synthesis, thereby elevating the levels of anthocyanins and other phenolic compounds in both red and yellow/green cultivars. PMID:23727590

  18. Knowing Apples

    Directory of Open Access Journals (Sweden)

    Lorraine Shannon

    2013-05-01

    Full Text Available This essay employs a first-person fictional narrator to explore the nature of human-plant relations through the example of Thoreau’s Wild Apples and enacts the transformational process necessary to write in conjunction with non-conscious vegetal life by paying attention to the unthought known of the vegetative soul.

  19. Apple Must

    OpenAIRE

    Ehmsen, Amalie Schultz; Larsen, Mikael; Brandt, Mike; Villadsen, Niels; Vinther, Sine Maria

    2014-01-01

    Abstract This project contains an analysis of Apple’s aesthetics, as presented through advertisement and the general aesthetic expression of their products. Combining Marxist theory of commodity aesthetics with Anthony Giddens’ and Abraham Maslow’s sociological theory, the paper endeavors to present a connection between the valorization of Apple’s products and the needs and wants of the late-modern consumer. To this end, a semiotic analysis of certain Apple commercials, followed by a herme...

  20. What's an Adam's Apple?

    Science.gov (United States)

    ... Skating Crushes What's a Booger? What's an Adam's Apple? KidsHealth > For Kids > What's an Adam's Apple? Print A A A Text Size You're ... the throat. This is what's called an Adam's apple. Everyone's larynx grows during puberty, but a girl's ...

  1. Biomarker Identification in Metabolomics of Dietary Studies on Apple and Apple Products

    DEFF Research Database (Denmark)

    Rago, Daniela

    diet supplemented with two dosages (5 g/day and 10g/day) of whole fresh apple pieces through 16 weeks (PAPER I). Plasma samples were analysed by LC-MS. Due to the complexity of the design of the study, several chemometric analyses have been employed. Initially, ASCA was applied in order to isolate the...... with possible exposure markers, including phase 2 metabolites of flavonoid degradation products and chalcones. Furthermore, in urine data, a larger number of markers increased after the apple and juice intervention - compared with control - respect to plasma. Overall, the studies included in this...

  2. Genetic characteristics of diversity of apple resistance to apple scab

    OpenAIRE

    Sikorskaitė-Gudžiūnienė, Sidona

    2014-01-01

    The aim of the research. To identify genes involved in V. inaequalis induced resistance response in Malus sp. and to develop apple hybrids with pyramidic resistance. Specific aims: 1. To characterize the genetic diversity and resistance to apple scab in the collection of apple genetic resources; 2. To develop apple hybrids of pyramidic resistance for apple breeding; 3. To characterize apple nuclear proteome and to perform a comparative genomic analysis of V. inaequalis induced Malus response;...

  3. What Makes Apple Golden

    Institute of Scientific and Technical Information of China (English)

    Betsy Morrish

    2008-01-01

    @@ The mass market is supposed to be dead, but you would nover know it from Apple.In Felmuny the iTunes Store became the second-largest music retailer in the U.S., right behind Wal-Mart.The iPod is to music players what Kleenex is to tissue or Xerox is to copiers.Almost everything Apple makes transcends gender, geography, age,and race.An Apple Store is a demographic melting pot, with computer games for kids and a Genius Bar for their parents and so much cool stuff to touch that it's a magnet for teens and twentysomethings.

  4. Apples vs. Brownies

    DEFF Research Database (Denmark)

    Hansen, Pelle Guldborg; Skov, Katrine Lund; Skov, Laurits Rohden; Jespersen, Andreas Maaløe; Schmidt, Karsten

    2015-01-01

    A standard food setting served daily by caterers at thousands of conference venues, meetings and gatherings is the coffee break buffet featuring some high-energy snack (e.g. brownies) as well as what often seems like an obligatory healthy option (e.g. apples). Knowing how the display of these food......-energy choice (brownies), (b) convenience of consuming the healthy option (apples) and (c) their relative presentation order....

  5. Control of apple scab (Venturia inaequalis) in organic apple growing. StopScab: A Danish research programme for screening substitutes to copper fungicides

    OpenAIRE

    M. Bengtsson; Hockenhull, J.

    2002-01-01

    The Danish research project StopScab (2002-2004) has recently been initiated to identify and begin to develop some new approaches and materials for the control of apple scab in organic growing. Four linked objectives are included: 1) screening of materials for scab control 2) histopathological investigation of host-pathogen interactions 3) orchard testing of selected control compounds and 4) characterisation of metabolite profiles in apples

  6. Apple otsib kohta mobiiliturul / Lauri Matsulevitsh

    Index Scriptorium Estoniae

    Matsulevitsh, Lauri

    2005-01-01

    Apple tahab siseneda mobiiliturule, demonstreeriti Motorola mudelit Rokr, mis mängib lugusid Apple'i Tunes'i online-muusikapoest. Diagramm: Apple'i aktsia. Vt. samas: Apple on Eesti investorite meelisaktsiaid

  7. Determination of diafenthiuron and its metabolites residue in apple by QuEChERS-high performance liquid chromatography-tandem mass spectrometry%QuEChERS-高效液相色谱-串联质谱法测定苹果中丁醚脲及其代谢物残留量

    Institute of Scientific and Technical Information of China (English)

    王利强; 葛含光; 王永芳; 葛宝坤

    2015-01-01

    目的:建立基于 QuEChERS-高效液相色谱-串联质谱测定苹果中的丁醚脲及其代谢物丁醚脲-脲和丁醚脲-甲酰胺残留量的方法。方法样品经乙腈提取后用PSA作为固相分散萃取剂净化除杂,以0.1%甲酸乙腈溶液和0.1%甲酸水溶液(含10 mmol/L甲酸铵)为流动相进行梯度洗脱,高效液相色谱-串联质谱法正离子多反应监测模式(MRM)测定。结果丁醚脲及其代谢物在5~100μg/L的范围内有良好的线性,相关系数(R2)>0.999。定量限5μg/kg为以空白苹果为基质,在5、10、50μg/kg三个水平的加标回收率范围在78.3%~103.1%之间,精密度在2.1%~8.2%之间。结论该方法前处理快速简单,可用于对进出口的苹果中丁醚脲及其代谢物的检测。%ABSTRACT:ObjectiveTo establish a method to detect the diafenthiuron and its metabolites residue in apple by QuEChERS-high performance liquid chromatography-tandem mass spectrometry.Methods The samples were extracted with acetonitrile and cleaned up by PSA. The separation was carried out with a gradient elution using 0.1% formic acid acetonitrile solution and 0.1% formic acid solution (10 mmol/L ammonium formate) as mobile phases. The analysis of diafenthiuron and its metabolites was performed under electrospray positive ionization MRM mode.Results A good linearity (R2>0.999) was achieved for the target compounds in the range of 5~100μg/L. The limit of quantification was 5μg/kg. The recoveries at the three spiked levels (5, 10, and 20μg/kg) in the blank matrix were ranged from 78.3% to103.1%, with the relative standard deviations varied from 2.1% to 8.2%. ConclusionThe method is accurate and efficient, and can be applied to the determination of diafenthiuron and its metabolites residue in apple for import and export.

  8. About APPLE II Operation

    International Nuclear Information System (INIS)

    The operation of an APPLE II based undulator beamline with all its polarization states (linear horizontal and vertical, circular and elliptical, and continous variation of the linear vector) requires an effective description allowing an automated calculation of gap and shift parameter as function of energy and operation mode. The extension of the linear polarization range from 0 to 180 deg. requires 4 shiftable magnet arrrays, permitting use of the APU (adjustable phase undulator) concept. Studies for a pure fixed gap APPLE II for the SLS revealed surprising symmetries between circular and linear polarization modes allowing for simplified operation. A semi-analytical model covering all types of APPLE II and its implementation will be presented

  9. About APPLE II Operation

    Science.gov (United States)

    Schmidt, T.; Zimoch, D.

    2007-01-01

    The operation of an APPLE II based undulator beamline with all its polarization states (linear horizontal and vertical, circular and elliptical, and continous variation of the linear vector) requires an effective description allowing an automated calculation of gap and shift parameter as function of energy and operation mode. The extension of the linear polarization range from 0 to 180° requires 4 shiftable magnet arrrays, permitting use of the APU (adjustable phase undulator) concept. Studies for a pure fixed gap APPLE II for the SLS revealed surprising symmetries between circular and linear polarization modes allowing for simplified operation. A semi-analytical model covering all types of APPLE II and its implementation will be presented.

  10. Apple Watch for dummies

    CERN Document Server

    Saltzman, Marc

    2015-01-01

    Your all-encompassing guide to the Apple Watch Are you an Apple Watch enthusiast and want to master all of its features to impress friends and intimidate enemies? Or perhaps you're a less-than-tech-savvy newcomer to the ""wearable"" craze and want to get the most out of it? In Apple Watch For Dummies, you'll discover how this incredible device does way more than simply tell time. Through hands-on, easy-to-follow instruction, you'll find out how to send and receive text messages and emails, use Siri, find movie times, access your favorite apps and get directions]. Plus, you'll get a handle on

  11. AppleScript

    CERN Document Server

    Munro, Mark Conway

    2010-01-01

    Developers will advance their AppleScript skills easily with this guide. Part of the Developer Reference series, this book is packed with professional secrets for designing and building automated solutions with AppleScript, the powerful, system-level scripting language built into every Mac. Programmers will discover new ways to increase their professional efficiency and become more valuable in their jobs. With up to date coverage of Mac OS X 10.6 Snow Leopard and loaded with real-world tips and techniques, this guide includes best practices and conventions along with informative lessons. You'l

  12. Apple TV for dummies

    CERN Document Server

    Chambers, Mark L

    2013-01-01

    Watch your iTunes downloads on a television screen with help from Apple TV For Dummies. This comprehensive guide offers shopping tips; easy-to-understand installation and setup directions; and advanced material like content creation, troubleshooting, and optimizing network speeds. You get the ""download"" on: Apple TV setup and customizing High-Definition video hardware State-of-the-art audio hardware Connecting both computer and video equipment Using iTunes and the iTunes Store Cataloging your multimedia library Setting u

  13. Sensory and metabolic profiles of "Fuji" apples (Malus domestica Borkh.) grown without synthetic agrochemicals: the role of ethylene production.

    Science.gov (United States)

    Tanaka, Fukuyo; Miyazawa, Toshio; Okazaki, Keiki; Tatsuki, Miho; Ito, Tsutae

    2015-01-01

    Flavors of "Fuji" apple cultivated with or without synthetic agrochemicals were compared using quantitative descriptive analyses (QDA) and metabolite profiling for 3 seasons. Experimental plots included conventional crops (with agrochemicals) and organic crops (without agrochemicals) at our institute and organic and conventional farms. Additionally, mass market samples were analyzed. Organic apples were weak in sweetness and floral characteristics and had enhanced green and sour flavors. Most esters and sugars were present in lower concentrations in organic than in conventional apples. Close relation of principal component 1 of QDA and metabolite profiles, to ethylene production suggested that ethylene is considerably involved in flavor synthesis. Reduced ethylene associated with immaturity accounted for insufficient flavor synthesis and weak aroma and flavor attributes of organic apples. Furthermore, organic apples from the farm were more flavorsome than those from the institute in 2012, suggesting possible recovery of ethylene production after a long organic cultivation period. PMID:26140475

  14. Metabolic and gene expression analysis of apple (Malus × domestica) carotenogenesis

    OpenAIRE

    Ampomah-Dwamena, Charles; Dejnoprat, Supinya; Lewis, David; Sutherland, Paul; Volz, Richard K; Andrew C. Allan

    2012-01-01

    Carotenoid accumulation confers distinct colouration to plant tissues, with effects on plant response to light and as well as health benefits for consumers of plant products. The carotenoid pathway is controlled by flux of metabolites, rate-limiting enzyme steps, feed-back inhibition, and the strength of sink organelles, the plastids, in the cell. In apple (Malus × domestica Borkh), fruit carotenoid concentrations are low in comparison with those in other fruit species. The apple fruit flesh,...

  15. Apple Shuns Tracking Tool

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Apple Inc. is advising software de- velopers to stop using a feature in software for its iPhones and iPads .that has been linked to privacyconcerns, a move that would also take away a widely used tool for tracking users and their behavior. Developers who write programs for Apple's lOS operating system have been using a unique.

  16. APPLE PHYTOCHEMICALS FOR HUMAN BENEFITS

    Directory of Open Access Journals (Sweden)

    R. D. Chakole

    2012-04-01

    Full Text Available Evidence suggests that a diet high in fruits and vegetables may decrease the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Apples are a widely consumed, rich source of phytochemicals, and epidemiological studies have linked the consumption of apples with reduced risk of some cancers, cardiovascular disease, asthma, and diabetes. In the laboratory, apples have been found to have very strong antioxidant activity, inhibit cancer cell proliferation, decrease lipid oxidation, and lower cholesterol. Apples contain a variety of phytochemicals, including quercetin, catechin, phloridzin and chlorogenic acid, all of which are strong antioxidants. The phytochemical composition of apples varies greatly between different varieties of apples, and there are also small changes in phytochemicals during the maturation and ripening of the fruit. Storage has little to no effect on apple phytochemicals, but processing can greatly affect apple phytochemicals. While extensive research exists, a literature review of the health benefits of apples and their phytochemicals has not been compiled to summarize this work. The purpose of this paper is to review the most recent literature regarding the health benefits of apples and their phytochemicals, phytochemical bioavailability and antioxidant behavior, and the effects of variety, ripening, storage and processing on apple phytochemicals

  17. New Insights on the Apple and Health

    DEFF Research Database (Denmark)

    Bügel, Susanne; Ravn-Haren, Gitte; Poulsen, Morten;

    , flavonoids and phenolic acids and because of the high intakes of apples in northern parts of Europe. A series of 4-16 w rat feeding studies with fresh whole apples, dried apple, apple puree, clear and cloudy apple juices, apple pomace, and apple pectins have been conducted. A human cross-over dietary......Regular consumption of fruits and vegetables is associated with reduced risks of certain cancers, cardiovascular diseases, stroke, Alzheimer disease etc. In this project, we focused on apples as a model fruit for some of this research due to its high contents of soluble and insoluble fibers...... feeding with apple or apple pectin. This was also reflected in changed gut flora enzymatic activities, whereas caecum short chain fatty acid concentrations were unaffected by feeding with all apple products, except high doses of apple pectins. In the human study the whole apple had the strongest...

  18. Processed Apple Product Marketing Analysis: Hard Cider and Apple Wine

    OpenAIRE

    Rowles, Kristin

    2000-01-01

    Hard cider and apple wine offer new value-added marketing opportunities to the apple industry. Both products are situated in rapidly growing categories of the beverage industry. The development of effective marketing strategies for these products requires an understanding of the forces driving competition in these markets. This paper provides background information to support competitive analysis and strategy development. Development of these markets will be positive for the apple industry, b...

  19. Equity Research - Apple

    OpenAIRE

    Santos, João Carlos Portela Marques dos

    2013-01-01

    O presente Trabalho Final de Mestrado tem como objectivo a avaliação da empresa norteamericana Apple Inc. de forma a obter um preço-alvo para as suas acções com referência a 29 de Setembro de 2012, data de fim do ano fiscal da empresa. O processo de avaliação da empresa envolveu, numa primeira fase, uma análise detalhada ao negócio da Apple (e suas perspectivas de crescimento) e ao seu posicionamento estratégico. Em segundo lugar, foram aplicadas técnicas de avaliação de empresas de forma a d...

  20. Apple Image Processing Educator

    Science.gov (United States)

    Gunther, F. J.

    1981-01-01

    A software system design is proposed and demonstrated with pilot-project software. The system permits the Apple II microcomputer to be used for personalized computer-assisted instruction in the digital image processing of LANDSAT images. The programs provide data input, menu selection, graphic and hard-copy displays, and both general and detailed instructions. The pilot-project results are considered to be successful indicators of the capabilities and limits of microcomputers for digital image processing education.

  1. Cedar-apple Rust

    Directory of Open Access Journals (Sweden)

    Clark L. Ovrebo

    2006-12-01

    Full Text Available The photograph on the cover illustrates a phenomenon of nature that can be seen in the Oklahoma springtime at about the same time that the redbuds are in flower and the morels are fruiting. The orange-colored masses represent a stage in the life cycle of cedar-apple rust, Gymnosporangium juniperi-virginianae, and this stage is occurring on the eastern red cedar (Juniperus virginiana.

  2. Apple iPhone

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Apple iPhone千呼万唤始出来。它结合多种功能于一体,具有网络、桌面级的电子邮件、网页浏览及地图搜索等功能。全新的用户界面基于一个大型综合触摸显示屏。

  3. Apple Grading Using Fuzzy Logic

    OpenAIRE

    KAVDIR, İsmail

    2003-01-01

    Classification is vital for the evaluation of agricultural produce. However, the high costs, subjectivity, tediousness and inconsistency associated with manual sorting have been forcing the post harvest industry to apply automation in sorting operations. Fuzzy logic (FL) was applied as a decision making support to grade apples in this study. Quality features such as the color, size and defects of apples were measured through different equipment. The same set of apples was graded by both a hum...

  4. Teach yourself visually Apple Watch

    CERN Document Server

    Hart-Davis, Guy

    2015-01-01

    Master your new smartwatch quickly and easily with this highly visual guide Teach Yourself VISUALLY Apple Watch is a practical, accessible guide to mastering the powerful features and functionality of your new smartwatch. For Apple devotees and new users alike, this easy-to-follow guide features visually rich tutorials and step-by-step instructions that show you how to take advantage of all of the Apple watch's capabilities. You'll learn how to track your health, control household devices, download and install apps, sync your music, sync other Apple devices, and efficiently use the current O

  5. Transcriptome profiling to identify genes involved in pathogenicity of Valsa mali on apple tree.

    Science.gov (United States)

    Ke, Xiwang; Yin, Zhiyuan; Song, Na; Dai, Qingqing; Voegele, Ralf T; Liu, Yangyang; Wang, Haiying; Gao, Xiaoning; Kang, Zhensheng; Huang, Lili

    2014-07-01

    Apple Valsa canker, caused by the fungus Valsa mali (Vm), is one of the most destructive diseases of apple in China. A better understanding of this host-pathogen interaction is urgently needed to improve management strategies. In the current study we sequenced the transcriptomes of Vm during infection of apple bark and mycelium grown in axenic culture using Illumina RNA-Seq technology. We identified 437 genes that were differentially expressed during fungal infection compared to fungal mycelium grown in axenic culture. One hundred and thirty nine of these 437 genes showed more than two fold higher transcript abundance during infection. GO and KEGG enrichment analyses of the up-regulated genes suggest prevalence of genes associated with pectin catabolic, hydrolase activity and secondary metabolite biosynthesis during fungal infection. Some of the up-regulated genes associated with loss of pathogenicity and reduced virulence annotated by host-pathogen interaction databases may also be involved in cell wall hydrolysis and secondary metabolite transport, including a glycoside hydrolase family 28 protein, a peptidase and two major facilitator superfamily proteins. This highlights the importance of secondary metabolites and cell wall hydrolases during establishment of apple Valsa canker. Functional verification of the genes involved in pathogenicity of Vm will allow us to better understand how the fungus interferes with the host machinery and assists in apple canker establishment. PMID:24747070

  6. At PS170 (APPLE)

    CERN Multimedia

    1983-01-01

    APPLE stands for Antiproton-Proton to Pair of LEptons (an acronym of the ancestor experiment PAPLEP), the PS170 experiment setup at LEAR to study e+e-pair production in antiproton-proton annihilation by Padova-(CEN) Saclay- Torino Collaboration. It consisted of a liquid hydrogen target surrounded by several layers of proportional chambers in the vertical field of a C-magnet (this photo), a gas Cerenkov counter, wire chambers, hodoscopes, and an electromagnetic calorimeter (see photo 8302539X, 8302540X). See also photo 8301539X for the setup assembly at an early stage.

  7. The gravity apple tree

    Science.gov (United States)

    Espinosa Aldama, Mariana

    2015-04-01

    The gravity apple tree is a genealogical tree of the gravitation theories developed during the past century. The graphic representation is full of information such as guides in heuristic principles, names of main proponents, dates and references for original articles (See under Supplementary Data for the graphic representation). This visual presentation and its particular classification allows a quick synthetic view for a plurality of theories, many of them well validated in the Solar System domain. Its diachronic structure organizes information in a shape of a tree following similarities through a formal concept analysis. It can be used for educational purposes or as a tool for philosophical discussion.

  8. Apple vs. Android

    DEFF Research Database (Denmark)

    Bjelland, Johannes; Canright, Geoff; Engø-Monsen, Kenth;

    have been difficult to collect, but in recent years researchers have gained access to massive social network data from e.g. online instant messaging services [8][5] and phone log data [2][4][3][6][9]. Such data has made it possible to study e.g. social churn [3], service uptake [2] among telecom...... can be measured by studying this network. In this paper, we do a comparative study of social spreading effects for two competing types of smartphones - the Apple iPhone, and smartphones based on Google’s Android OS....

  9. Apple Inc. : equity valuation

    OpenAIRE

    Alexandre, Gonçalo Lopes

    2016-01-01

    This dissertation presents a valuation of Apple Inc., an American company that sits amongst the largest companies in the world, in market capitalization terms. Although it started as a computer company, back in 1976, nowadays it is best known for its smartphone flagship – the iPhone, introduced in 2007, it revolutionized the entire mobile phone industry. Today, the iPhone represents about 66% of total sales, however there are other products in Apple’s product line that are considered by a lar...

  10. Phytosterol conjugation in cold-stored apple fruit is linked to oxidative stress and ripening

    Science.gov (United States)

    Low temperature stress is implicated in a wide-range of apple fruit postharvest necrotic disorders. Previously, untargeted metabolic profiling identified alterations in multiple metabolic processes that precede superficial scald symptom development. Metabolites with free sterol (FS) –like mass spe...

  11. Phytosterol conjugation in cold-storage apple fruit is linked to oxidative stress and ripening

    Science.gov (United States)

    Low temperature stress is implicated in a wide-range of apple fruit postharvest necrotic disorders. Previously, untargeted metabolic profiling identified alterations in multiple metabolic processes that precede superficial scald symptom development. Metabolites with free sterol (FS) –like mass spe...

  12. Study of Cyclodextrin-Based Polymers to Extract Patulin from Apple Juice

    Science.gov (United States)

    Synthetic sorbents offer a means to develop more robust materials to detect analytes in complex matrices, including methods to detect naturally occurring contaminants in agricultural commodities. Patulin is a mold metabolite associated with rotting apples and poses health risks to humans and animal...

  13. Uptake of a fluorescent L-glucose derivative 2-NBDLG into three-dimensionally accumulating insulinoma cells in a phloretin-sensitive manner.

    Science.gov (United States)

    Sasaki, Ayako; Nagatomo, Katsuhiro; Ono, Koki; Yamamoto, Toshihiro; Otsuka, Yuji; Teshima, Tadashi; Yamada, Katsuya

    2016-01-01

    Of two stereoisomers of glucose, only D- and not L-glucose is abundantly found in nature, being utilized as an essential fuel by most organisms. The uptake of D-glucose into mammalian cells occurs through glucose transporters such as GLUTs, and this process has been effectively monitored by a fluorescent D-glucose derivative 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) at the single cell level. However, since fluorescence is an arbitrary measure, we have developed a fluorescent analog of L-glucose 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-L-glucose (2-NBDLG), as a negative control substrate for more accurately identifying the stereoselectivity of the uptake. Interestingly, a small portion of mouse insulinoma cells MIN6 abundantly took up 2-NBDLG at a late culture stage (≳ 10 days in vitro, DIV) when multi-cellular spheroids exhibiting heterogeneous nuclei were formed, whereas no such uptake was detected at an early culture stage (≲ 6 DIV). The 2-NBDLG uptake was persistently observed in the presence of a GLUT inhibitor cytochalasin B. Neither D- nor L-glucose in 50 mM abolished the uptake. No significant inhibition was detected by inactivating sodium/glucose cotransporters (SGLTs) with Na(+)-free condition. To our surprise, the 2-NBDLG uptake was totally inhibited by phloretin, a broad spectrum inhibitor against transporters/channels including GLUTs and aquaporins. From these, a question might be raised if non-GLUT/non-SGLT pathways participate in the 2-NBDLG uptake into spheroid-forming MIN6 insulinoma. It might also be worthwhile investigating whether 2-NBDLG can be used as a functional probe for detecting cancer, since the nuclear heterogeneity is among critical features of malignancy. PMID:26553070

  14. 7 CFR 33.5 - Apples.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Apples. 33.5 Section 33.5 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... ISSUED UNDER AUTHORITY OF THE EXPORT APPLE ACT Definitions § 33.5 Apples. Apples mean fresh whole...

  15. Volatile Metabolites

    Directory of Open Access Journals (Sweden)

    Daryl D. Rowan

    2011-11-01

    Full Text Available Volatile organic compounds (volatiles comprise a chemically diverse class of low molecular weight organic compounds having an appreciable vapor pressure under ambient conditions. Volatiles produced by plants attract pollinators and seed dispersers, and provide defense against pests and pathogens. For insects, volatiles may act as pheromones directing social behavior or as cues for finding hosts or prey. For humans, volatiles are important as flavorants and as possible disease biomarkers. The marine environment is also a major source of halogenated and sulfur-containing volatiles which participate in the global cycling of these elements. While volatile analysis commonly measures a rather restricted set of analytes, the diverse and extreme physical properties of volatiles provide unique analytical challenges. Volatiles constitute only a small proportion of the total number of metabolites produced by living organisms, however, because of their roles as signaling molecules (semiochemicals both within and between organisms, accurately measuring and determining the roles of these compounds is crucial to an integrated understanding of living systems. This review summarizes recent developments in volatile research from a metabolomics perspective with a focus on the role of recent technical innovation in developing new areas of volatile research and expanding the range of ecological interactions which may be mediated by volatile organic metabolites.

  16. APPLE PHYTOCHEMICALS FOR HUMAN BENEFITS

    OpenAIRE

    R. D. Chakole; Azhar Ahmed; Manoj S. Charde

    2012-01-01

    Evidence suggests that a diet high in fruits and vegetables may decrease the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Apples are a widely consumed, rich source of phytochemicals, and epidemiological studies have linked the consumption of apples with reduced risk of some cancers, cardiovascular disease, asthma, and diabe...

  17. The APPL "Learning Map"

    Science.gov (United States)

    2005-01-01

    Root Learning, a learning consulting organization with a background in strategic planning, recognizes the knowledge gap that frequently exists between a leadership team and the rest of an organization. Team members supposedly working toward the same goal don't always have the same vision as to where the organization is headed, and they may not understand how the piece they are accountable for fits into the big picture. To address these complex problems, Root Learning utilizes the age-old tools of sarcasm, metaphor and graphics (much in the same way that ASK uses a traditional storytelling format.) The company is best known for creating "Learning Maps" like this one: humorous drawings based on the inner workings of an organization. Their purpose is to put complex topics on the table, to stimulate discussion, and to ultimately give team members a common vision of where the organization is going and what role they personally play in getting there. APPL knows how effective it is to incorporate new and engaging techniques into its knowledge sharing programs. By collaborating with Root Learning, we were able to expand the knowledge of the organization and add one more of these techniques to our repertoire.

  18. Tests of biological activity of metabolites from Penicillium expansum (Link Thom various isolates

    Directory of Open Access Journals (Sweden)

    Halina Borecka

    2013-12-01

    Full Text Available Aqrobacterium tumefaciens and cucumber, mustard and linseeds were compared as test organisms for evaluation of the biological activity of patulin. It was found that the reaction of cucumber seeds and linseed to the patulin concentrations was more pronounced than that of mustard and Aqrobacterium tumefaciens. The activity of metabolites produced by Penicillium expansum was investigated with the use of cucumber seeds. As measure of activity served the percentage of radicule growth inhibition was compared with the growth in control seeds. The biological activity of the metabolites was specific for the isolates, those from apples being more active. Thirty two isolates from pears and 34 from apples were examined.

  19. Identification, characterization and mycotoxigenic ability of Alternaria spp. causing core rot of apple fruit in Greece.

    Science.gov (United States)

    Ntasiou, Panagiota; Myresiotis, Charalampos; Konstantinou, Sotiris; Papadopoulou-Mourkidou, Euphemia; Karaoglanidis, George S

    2015-03-16

    Alternaria core rot is a major postharvest disease of apple fruit in several countries of the world, including Greece. The study was conducted aiming to identify the disease causal agents at species level, investigate the aggressiveness of Alternaria spp. isolates and the susceptibility of different apple varieties and determine the mycotoxigenic potential of Alternaria spp. isolates from apple fruit. Seventy-five Alternaria spp. isolates obtained from apple fruit showing core rot symptoms were identified as either Alternaria tenuissima or Alternaria arborescens at frequencies of 89.3 and 11.7%, respectively, based on the sequence of endopolygalacturonase (EndoPG) gene. Artificial inoculations of fruit of 4 different varieties (Fuji, Golden Delicious, Granny Smith and Red Delicious) and incubation at two different temperatures (2 and 25°C) showed that fruit of Fuji variety were the most susceptible and fruit of Golden Delicious the most resistant to both pathogens. In addition, the production of 3 mycotoxins, alternariol (AOH), alternariol monomethyl ether (AME) and tentoxin (TEN) was investigated in 30 isolates of both species. Mycotoxin determination was conducted both in vitro, on artificial nutrient medium and in vivo on artificially inoculated apple fruit, using a high performance liquid chromatography with diode array detector (HPLC-DAD). The results showed that most of the isolates of both species were able to produce all the 3 metabolites both in vivo and in vitro. On apple fruit A. tenuissima isolates produced more AOH than A. arborescens isolates, whereas the latter produced more TEN than the former. Such results indicate that Alternaria core rot represents a major threat of apple fruit production not only due to quantitative yield losses but also for qualitative deterioration of apple by-products. PMID:25560914

  20. Biotechnology and apple breeding in Japan

    OpenAIRE

    Igarashi, Megumi; Hatsuyama, Yoshimichi; Harada, Takeo; Fukasawa-Akada, Tomoko

    2016-01-01

    Apple is a fruit crop of significant economic importance, and breeders world wide continue to develop novel cultivars with improved characteristics. The lengthy juvenile period and the large field space required to grow apple populations have imposed major limitations on breeding. Various molecular biological techniques have been employed to make apple breeding easier. Transgenic technology has facilitated the development of apples with resistance to fungal or bacterial diseases, improved fru...

  1. Big Book of Apple Hacks

    CERN Document Server

    Seibold, Chris

    2008-01-01

    Bigger in size, longer in length, broader in scope, and even more useful than our original Mac OS X Hacks, the new Big Book of Apple Hacks offers a grab bag of tips, tricks and hacks to get the most out of Mac OS X Leopard, as well as the new line of iPods, iPhone, and Apple TV. With 125 entirely new hacks presented in step-by-step fashion, this practical book is for serious Apple computer and gadget users who really want to take control of these systems. Many of the hacks take you under the hood and show you how to tweak system preferences, alter or add keyboard shortcuts, mount drives and

  2. Genetic Diversity of a Natural Population of Apple stem pitting virus Isolated from Apple in Korea

    OpenAIRE

    Yoon, Ju Yeon; Joa, Jae Ho; Choi, Kyung San; Do, Ki Seck; Lim, Han Cheol; Chung, Bong Nam

    2014-01-01

    Apple stem pitting virus (ASPV), of the Foveavirus genus in the family Betaflexiviridae, is one of the most common viruses of apple and pear trees. To examine variability of the coat protein (CP) gene from ASPV, eight isolates originating from 251 apple trees, which were collected from 22 apple orchards located in intensive apple growing areas of the North Gyeongsang and North Jeolla Provinces in Korea, were sequenced and compared. The nucleotide sequence identity of the CP gene of eight ASPV...

  3. Antioxidant activity of polyphenol-enriched apple juice

    OpenAIRE

    Šumić Zdravko M.; Tepić Aleksandra N.; Savatović Slađana M.; Nikolić Milan S.

    2009-01-01

    This paper shows that it is possible to improve antioxidant activity of apple juice by extraction of polyphenolic compounds from apple pomace, as waste, and their addition to the apple juice. Raw apple juice was prepared by pressing of apple mash. After thermal treatment of raw apple juice, depectinisation, additional clarification and filtration, the clarified juice was obtained. In raw and clarified apple juice soluble solids, acidity, reducing sugar, total sugars and brown component conten...

  4. Molecular characterization of genes encoding leucoanthocyanidin reductase involved in proanthocyanidin biosynthesis in apple

    Directory of Open Access Journals (Sweden)

    Yuepeng eHan

    2015-04-01

    Full Text Available Proanthocyanidins (PAs are the major component of phenolics in apple, but mechanisms involved in PA biosynthesis remain unclear. Here, the relationship between the PA biosynthesis and the expression of genes encoding leucoanthocyanidin reductase (LAR and anthocyanidin reductase (ANR was investigated in fruit skin of one apple cultivar and three crabapples. Transcript levels of LAR1 and ANR2 genes were significantly correlated with the contents of catechin and epicatechin, respectively, which suggests their active roles in PA synthesis. Surprisingly, transcript levels for both LAR1 and LAR2 genes were almost undetectable in two crabapples that accumulated both flavan-3-ols and PAs. This contradicts the previous finding that LAR1 gene is a strong candidate regulating the accumulation of metabolites such as epicatechin and PAs in apple. Ectopic expression of apple MdLAR1 gene in tobacco suppresses expression of the late genes in anthocyanin biosynthetic pathway, resulting in loss of anthocyanin in flowers. Interestingly, a decrease in PA biosynthesis was also observed in flowers of transgenic tobacco plants overexpressing the MdLAR1 gene, which could be attributed to decreased expression of both the NtANR1 and NtANR2 genes. Our study not only confirms the in vivo function of apple LAR1 gene, but it is also helpful for understanding the mechanism of PA biosynthesis.

  5. Resistance management in Vf apple scab resistant organic apple orchards

    OpenAIRE

    Trapman, Marc

    2006-01-01

    Modern Vf scab resistant apple varieties open the way for organic growers to lower fungicide input, higher yields, better skin quality, more biological control for mites and insect pests and better consumer acceptance of their management practices. Manny examples in the past years have shown however that the Vf resis-tance can be easily overcome by local scab populations in north-western Europe. Discussions during the meetings of the IOBC working group Diseases in Orchards in 2000 in Fontevr...

  6. FACTORS EFFECTING TO THE AMOUNT OF PATULIN IN APPLE AND APPLE PRODUCTS

    Directory of Open Access Journals (Sweden)

    Çetin KADAKAL

    2000-01-01

    Full Text Available Patulin is a mycotoxin produced by several species of Penicillium, Aspergillus and Byssochlamys. In nature, it is found diffusely at various products and also in apple and apple products. Because of the negative effects of patulin on human health, ıt ıs started to be used as an important quality parameter especially in apple juice and various apple products. On the other hand, having water solubility and stability to the heat treatment properties acquired another importance to the patulin in apple juice and apple juice concentrate. Removal of rotten parts of apples that will be processed to the apple juice and apple juice concentrate made the end product reliable in view of patulin. At apple juice and apple juice concentrate factories, activated charcoal is used diffusely for the removal of patulin passed to the apple juice. Exterior of activated charcoal, with the addition of ascorbic acid and/or sorbates, sulfhydryl (SH components or different food ingredients (cinnamon oil, potassium sorbate e.t.c and treatment of low dose radiation and modified atmosphere to the apple juice were being effective at the reduction of patulin. There are contradictory results about the inactivation of patulin with heat treatment and storage.

  7. Effects of apples and specific apple components on the cecal environment of conventional rats: Role of apple pectin

    DEFF Research Database (Denmark)

    Licht, Tine Rask; Hansen, Max; Bergström, Anders;

    2010-01-01

    Background: Our study was part of the large European project ISAFRUIT aiming to reveal the biological explanations for the epidemiologically well-established health effects of fruits. The objective was to identify effects of apple and apple product consumption on the composition of the cecal...... study (14 weeks), while no effects of apple juice, puree or pomace on microbial composition in cecum were observed. Administration of either 0.33 or 3.3% apple pectin in the diet resulted in considerable changes in the DGGE profiles. A 2-fold increase in the activity of beta-glucuronidase was observed......-glucuronidase producing Clostridiales, and decreases the population of specific species within the Bacteroidetes group in the rat gut. Similar changes were not caused by consumption of whole apples, apple juice, puree or pomace....

  8. �Sauron� Apple Cultivar

    Directory of Open Access Journals (Sweden)

    Radu SESTRAS

    2010-06-01

    Full Text Available The new apple cultivar �Sauron� was obtained at Fruit Research Station Cluj, Romania (FRS Cluj, and homologated in 2007. �Sauron� was identified in open pollinated population of �Cluj 3/83� selection [�Cluj III-VI-5-26� selection (�Parmain d�Or�, open pollinated x �NJ 46�]. The trees have moderate vigour, upright then spread, with moderate productivity. Fruits are medium to large, usually red, with a portion being greenish or yellow-green and purple red vertically striped. The fruit has good quality, being soft eating apple due to their lack of crispness. Quality indices include firmness, crispness and excellent flavour. Fruits become ripe between 15 August and 5 September and the fruits are good quality, being proper for dessert and industrialization (juice, applesauce, pies, and cider.

  9. Kaolin particle films suppress many apple pests, disrupt natural enemies and promote woolly apple aphid

    NARCIS (Netherlands)

    Markó, V.; Blommers, L.H.M.; Bogya, S.; Helsen, H.H.M.

    2008-01-01

    Multiple applications of hydrophobic kaolin particle film in apple orchards suppressed numbers of blossom weevil (Anthonomus pomorum), brown leaf weevil (Phyllobius oblongus), attelabid weevil (Caenorhinus pauxillus), leafhoppers (Empoasca vitis and Zygina flammigera) and green apple aphid (Aphis po

  10. More on apples and tomatoes

    International Nuclear Information System (INIS)

    The study of the context of different kinds of massive stars (apples and tomatoes) can provide insights on their evolutionary status. Useful constraints on the models can be obtained provided that visual and bolometric magnitudes of the different components are carefully determined. Here, evidence is reviewed that massive stars spend their short lives in tight clusters. Reddening determinations and bolometric corrections for these stars are briefly examined. 15 refs

  11. FACTORS EFFECTING TO THE AMOUNT OF PATULIN IN APPLE AND APPLE PRODUCTS

    OpenAIRE

    Çetin KADAKAL; Nas, Sebahattin

    2000-01-01

    Patulin is a mycotoxin produced by several species of Penicillium, Aspergillus and Byssochlamys. In nature, it is found diffusely at various products and also in apple and apple products. Because of the negative effects of patulin on human health, ıt ıs started to be used as an important quality parameter especially in apple juice and various apple products. On the other hand, having water solubility and stability to the heat treatment properties acquired another importance to the patulin in...

  12. Apple extracts as modulators of DNA methylation and inducers of Nrf2-ARE-associated antioxidant defense

    International Nuclear Information System (INIS)

    In the focus of the first part of this work was the modulation of the promoter methylation of the tumor suppressor gene p16INK4a by an extract of Bohnapfel (BAE) as well as its main constituent n-chlorogenic acid (CGA) in both, human colon carcinoma cells (HT29) and during a pilot intervention trial with type-II diabetic patients. BAE as well as CGA partially displayed a decrease in DNA methylation of the p16INK4a promoter after 48 h and 96 h incubation, however not in a time- and concentration-dependent manner. In addition, none of the two test compounds showed a re-activation of the tumor suppressor on both, transcription and protein levels. In a pilot trial with type-II diabetics 2 of 15 subjects reflected a methylation of the p16INK4a promoter in exfoliated colonocytes of human feaces. After four weeks of intervention with cloudy apple juice a reduction of the p16INK4a promoter methylation was observed in these two subjects. The focus of the second section of this thesis was the question whether polyphenol-rich apple pomace extracts (AT), obtained from depectinized pomace, are able to affect the intracellular redox state of HT29 cells allowing the classification of their potency compared to similarly prepared extracts of apple juice (AS). The studies showed that AT possesses a significantly higher bioactivity than AS regarding the reduction of intracellular ROS as well as the increase of the transcription of the enzyme glutamylcysteinligase (GCL). Further investigations indicated an activation of the Nrf2-ARE pathway and a downstream induction of selected enzymes of the phase-II metabolism. In the course of the activity-guided fractionation phloretin and quercetin could be identified as constituents, probably contributing to the total activity of the native AT. Nonetheless, a presence of yet unidentified constituents, possessing a high bioactivity seems to be likely, since the effect of the native extract cannot be explained by the effects reflected by the so

  13. Apples in the Alps aid atomic research

    International Nuclear Information System (INIS)

    The use of 2 Apple microcomputers by the radiation protection group working with CERN's Super Proton Synchrotron (SPS) is described. The first Apple is used primarily to process the data from the radiation monitoring system around the ring before the maintenance engineers can commence work on the SPS. The second Apple is being developed to help in the calibration of the ring's 1,100 monitors. (U.K.)

  14. AppleScript The Missing Manual

    CERN Document Server

    Goldstein, Adam

    2009-01-01

    AppleScript: The Missing Manual is every beginner's guide to learning the Macintosh's ultimate scripting tool: AppleScript. Through dozens of hands-on scripting examples, this comprehensive guide ensures that anyone including novices can learn how to control Mac applications in timesaving and innovative ways. Thanks to AppleScript: The Missing Manual, the path from regular Mac fan to seasoned scripter has never been easier.

  15. Analýza akcie Apple

    OpenAIRE

    Kereibayev, Alisher

    2014-01-01

    Bachelor thesis is dedicated to the analysis of Apple stock title. Analysis of stock title is provided on the basis of methods of fundamental and technical analysis, taking into account sectoral and fundamental analysis. The main objective of this thesis is to derive investment recommendations in relation to the stock Apple title, based on the evaluation of the related fundamental and technical factors on the price of the shares it operates. The hypothesis is the following statement: Apple sh...

  16. SELECTION AND CREATION OF APPLE VARIETIES FOR JUICE PRODUCTION

    OpenAIRE

    E.N. Sedov; N.S. Levgerova; E.S. Salina; Z.M. SEROVA

    2010-01-01

    The data of many years investigations on selection of apple varieties for juice production are presented. The new varieties, immune to apple scab, deserve the high label. The promising selection of apple for formation of rough gardens was shown

  17. New research to support the Danish organic apple production

    OpenAIRE

    Bertelsen, Marianne

    2012-01-01

    Experiments with new organic apple varieties, flower patches in the orchard, development of a new gas burner and apple storage conditions are new research initiatives designed to strengthen the production of organic apples.

  18. Seasonal variation in food allergy to apple

    DEFF Research Database (Denmark)

    Skamstrup Hansen, K; Vieths, S; Vestergaard, H;

    2001-01-01

    The aim of the study was to investigate the possibility of a seasonal variation in reactivity to apples in 27 birch pollen allergic patients. Before and during the birch pollen season 1998, the patients were subjected to double-blind, placebo-controlled food challenges (DBPCFCs) with grated fresh...... Golden Delicious apple followed by an open food challenge with whole fresh apple. The clinical reactions elicited during the challenges were evaluated both by the patients and the investigators. Moreover, the skin reactivity and the in vitro reactivity to apple were evaluated by skin prick test (SPT...

  19. What would Apple do? how you can learn from Apple and make money

    CERN Document Server

    Beckmann, Dirk

    2013-01-01

    In 2011, Apple officially became the most valuable company of all time. iPod, iPad, iTunes, App Store? the list goes on. Apple's must-have products add up to one giant success story. So what's their secret? What makes Apple the most innovative company on the planet? The answer: Apple does exactly the opposite of what any other company would do. Unlike the competition, Apple develops devices and programs by concentrating on a small number of functions. Forget complex market analyses. Forget asking customers to help develop products. And, unlike Google and other internet giants, it wants y

  20. Effect of apple varieties and irradiation on the quality of explosion puffed apple slices

    International Nuclear Information System (INIS)

    Many factors affect the quality of explosion puffed apple slices. Apple varieties of Guoguang, Fuji, Hongxiangjiao and Huangxiangjiao were used as materal, the quality of fresh apple and explosion puffed products were analyzed based on the data of their physical and chemical characteristics and flavor. The results showed that Guoguang apple had the optimal flavor and Huangxiangjiao apple had the optimal product quality. So the Guoguang apple was selected as the material for the following results showed that irradiation could soften apple tissue, improve the pre-drying rate, increase products crispness, and also reduce the products hardness, but the irradiation of 2 and 5 kGy could turn products brown seriously, which reduced the product quality. (authors)

  1. Instrumental measurements to investigate apple mealy texture

    Directory of Open Access Journals (Sweden)

    J. De Baerdemaeker

    1996-06-01

    Full Text Available Four apples varieties (Braebum, Elstar, Jonagold and Teser T219 were tested to study techniques for instrumental measurement of the mealiness of apple fruit. The measurements were done just after cold storage (2°C, 95% relative humidity and repeated one week of storage at 20 °C, 65% relative humidity later. The non-destructive acoustic response technique was used as a measure of fruit firmness. Texture profile analysis (TPA and tensile tests were applied to measure the hardness, cohe-siveness and adhesion of the apple tissue. The juiciness, soluble solids content (SSC, pH value and internal air space (IAS of the apples were determined. The results show that the TPA technique and tensile test may be derived as mechanical ways to measure apple mealiness. Apple fruit internal air space increases and juiciness decreases as the apple becomes mealy, and they may be good indications of mealiness. The mealiness criteria may be different for different varieties of apples.

  2. SED/Apple Computer, Inc., Partnership Program.

    Science.gov (United States)

    Stoll, Peter F.

    1991-01-01

    In 1990, the New York State Education Department (SED), Apple Computer, Inc., Boards of Cooperative Educational Services (BOCES), and school districts formed a partnership to explore the contribution technology can make to schools based on Apple Computer's Learning Society and SED's Long-Range Plan for Technology in Elementary and Secondary…

  3. Apple wine processing with different nitrogen contents

    Directory of Open Access Journals (Sweden)

    Aline Alberti

    2011-06-01

    Full Text Available The aim of this work was to evaluate the nitrogen content in different varieties of apple musts and to study the effect of different nitrogen concentrations in apple wine fermentation. The average total nitrogen content in 51 different apples juices was 155.81 mg/L, with 86.28 % of the values above 100 mg/L. The apple must with 59.0, 122.0 and 163.0 mg/L of total nitrogen content showed the maximum population of 2.05x 10(7; 4.42 x 10(7 and 8.66 x 10(7 cell/mL, respectively. Therefore, the maximum fermentation rates were dependent on the initial nitrogen level, corresponding to 1.4, 5.1 and 9.2 g/L.day, respectively. The nitrogen content in the apple musts was an important factor of growth and fermentation velocity.

  4. Metabolic and gene expression analysis of apple (Malus x domestica) carotenogenesis.

    Science.gov (United States)

    Ampomah-Dwamena, Charles; Dejnoprat, Supinya; Lewis, David; Sutherland, Paul; Volz, Richard K; Allan, Andrew C

    2012-07-01

    Carotenoid accumulation confers distinct colouration to plant tissues, with effects on plant response to light and as well as health benefits for consumers of plant products. The carotenoid pathway is controlled by flux of metabolites, rate-limiting enzyme steps, feed-back inhibition, and the strength of sink organelles, the plastids, in the cell. In apple (Malus × domestica Borkh), fruit carotenoid concentrations are low in comparison with those in other fruit species. The apple fruit flesh, in particular, begins development with high amounts of chlorophylls and carotenoids, but in all commercial cultivars a large proportion of this is lost by fruit maturity. To understand the control of carotenoid concentrations in apple fruit, metabolic and gene expression analysis of the carotenoid pathway were measured in genotypes with varying flesh and skin colour. Considerable variation in both carotenoid concentrations and compound profile was observed between tissues and genotypes, with carotenes and xanthophylls being found only in fruit accumulating high carotenoid concentrations. The study identified potential rate-limiting steps in carotenogenesis, which suggested that the expression of ZISO, CRTISO, and LCY-ε, in particular, were significant in predicting final carotenoid accumulation in mature apple fruit. PMID:22717407

  5. Utilization of pomace from apple processing industries: a review

    OpenAIRE

    Shalini, Rachana; Gupta, D K

    2010-01-01

    In large scale apple juice industry, about 75% of apple is utilized for juice and the remaining 25% is the by-product, apple pomace. In India, total production of apple pomace is about 1 million tons per annum and only approximately 10,000 tons of apple pomace is being utilized. Generally, apple pomace is thrown away, which causes environmental pollution. As the pomace is a part of fruit, it has potential for being converted into edible products. Apple pomace is a rich source of carbohydrate,...

  6. Double-blind, placebo-controlled food challenge with apple

    DEFF Research Database (Denmark)

    Skamstrup Hansen, K; Vestergaard, H; Stahl Skov, P;

    2001-01-01

    The aim of the study was to develop and evaluate different methods of double-blind, placebo-controlled food challenge (DBPCFC) with apple. Three different DBPCFC models were evaluated: fresh apple juice, freshly grated apple, and freeze-dried apple powder. All challenges were performed outside the...... high frequency of reactions to placebo, probably due to the ingredients used for blinding. The sensitivity of the models with freshly grated apple and freeze-dried apple powder was 0.74/0.60. An increase in sensitivity is desirable. The freeze-dried apple powder proved to be useful for SPT, HR, and...

  7. Advances in apple culture worldwide

    Directory of Open Access Journals (Sweden)

    Terence Robinson

    2011-10-01

    Full Text Available Over the last 60 years, planting densities for apple have increased as improved management systems have been developed. Dwarfing rootstocks have been the key to the dramatic changes in tree size, spacing and early production. The Malling series of dwarfing rootstocks (M.9 and M.26 have been the most important dwarfing rootstocks in the world but are poorly adapted in some areas of the world and they are susceptible to the bacterial disease fire blight and the soil disease complex, apple replant disease which limits their uses in some areas. Rootstock breeding programs in several parts of the world are developing improved rootstocks with resistance to fire blight, and replant disease, and improved cold hardiness and yield efficiency. A second important trend has been the increasing importance of new cultivars. New cultivars have provided opportunities for higher prices until they are over-produced. A new trend is the "variety club" in which variety owners manage the production and marketing of a new unique cultivar to bring higher prices to the growers and variety owners. This has led to many fruit growers being unable to plant or grow some new cultivars. Important rootstock and cultivar genes have been mapped and can be used in marker assisted selection of future rootstock and cultivar selections. Other important improvements in apple culture include the development of pre-formed trees, the development of minimal pruning strategies and limb angle bending which have also contributed to the dramatic changes in early production in the 2nd-5th years after planting. Studies on light interception and distribution have led to improved tree forms with better fruit quality. Simple pruning strategies and labor positioning platform machines have resulted in partial mechanization of pruning which has reduced management costs. Improved plant growth regulators for thinning and the development of a thinning prediction model based on tree carbohydrate balance

  8. Quanto Jobs c'è in Apple?

    OpenAIRE

    Franco Tommasi

    2011-01-01

    ItMolti vizi e virtù di Apple hanno le radici in vizi e virtù di Steve Jobs. Questi a loro volta derivano dalla spiccata originalità del suo percorso creativo e professionale e dai problemi piuttosto pressanti che questa ha creato. Ad essi egli ha reagito secondo la sua natura, determinando, in ultima analisi, la fisionomia caratteristica di Apple. Il testo seguente cercherà di illustrare alcuni punti di questa tesi.EnVices and virtues of Apple are rooted in vices and virtues of Steve Jobs. T...

  9. Experimental Study on Concentrating Apple Juice by Microwave

    OpenAIRE

    Ge Xinfeng

    2014-01-01

    In order to use microwave in concentrating apple juice and find the law of the relationship between the microwave and the concentrating effect and apply microwave to concentrate the apple juice, studied the apple juice concentrating process by microwave. The results show, microwave can effectively remove the moisture in apple juice and the concentrating quality and concentrating effect would not be affected. The process provides the basis that the microwave is used in apple juice concentrating.

  10. Vield and Quality of New Scab-Resistant Apple Varieties

    OpenAIRE

    Ruess, Franz

    2002-01-01

    125 scab-resistant or scab-tolerant apple varieties are currently being tested concerning their suitabiltity lor commerciai apple production at the research-station in Heuchlingen, Southern Germany. Apart Irom the scab-resistant apple varielies .Topaz' and .GoldRush' which are already used lor ecological apple production lurther varieties also seem lo be suitable. The aim 01 Ihe study was lo lind suilable scab-resistanl subslitutes lor not scab-resistanl standard apple-varielies lor ali perio...

  11. RESPONSE OF SEVERAL APPLE VARIETIES TO APPLE SCAB (VENTURIA INAEQUALIS) ATTACK IN CENTRAL TRANSYLVANIA CONDITIONS

    OpenAIRE

    R SESTRAS

    2003-01-01

    Among 75 varieties of apple, verified at the Fruit Research Station in Cluj-Napoca, Central Transylvania, Romania, during seven years (between 1990-1996), 38 registered with certain attack degree with apple scab on leaves and fruits. Susceptible on apple scab – Venturia inaequalis (Cke.) Wint. – were the varieties with Japanese origin (i.e. Fuji Aki-fu, Nebuta, Sekai ichi), and in certain years even well known cultivars, as Kaltherer Böhmer, Starkrimson, Red Delicious, Mutzu, Wellspur, Jersey...

  12. �Saruman� Apple Cultivar

    Directory of Open Access Journals (Sweden)

    Radu SESTRAS

    2010-06-01

    Full Text Available The apple cultivar �Saruman� was obtained at Fruit Research Station Cluj, Romania (FRS Cluj, and homologated in 2007. �Saruman� was released by hybridization between �Cluj III-VI-5-26� selection (�Parmain d�Or�, open pollinated and �NJ 46�. The trees are vigorous, spreading shape, and with medium crop yield. The fruits have large size, conic shape and mostly red (purple coloration; they have white flesh with a sweet, crisp, aromatic flavour and low acidity. Fruits become ripe in the last decade of August, first decade of September and the fruits are proper for dessert and well suited for cooking, applesauce, cider, pies.

  13. Enhanced metabolite generation

    Science.gov (United States)

    Chidambaram, Devicharan

    2012-03-27

    The present invention relates to the enhanced production of metabolites by a process whereby a carbon source is oxidized with a fermentative microbe in a compartment having a portal. An electron acceptor is added to the compartment to assist the microbe in the removal of excess electrons. The electron acceptor accepts electrons from the microbe after oxidation of the carbon source. Other transfers of electrons can take place to enhance the production of the metabolite, such as acids, biofuels or brewed beverages.

  14. Quanto Jobs c'è in Apple?

    Directory of Open Access Journals (Sweden)

    Franco Tommasi

    2011-12-01

    Full Text Available ItMolti vizi e virtù di Apple hanno le radici in vizi e virtù di Steve Jobs. Questi a loro volta derivano dalla spiccata originalità del suo percorso creativo e professionale e dai problemi piuttosto pressanti che questa ha creato. Ad essi egli ha reagito secondo la sua natura, determinando, in ultima analisi, la fisionomia caratteristica di Apple. Il testo seguente cercherà di illustrare alcuni punti di questa tesi.EnVices and virtues of Apple are rooted in vices and virtues of Steve Jobs. These, in turn, stem from the striking uniqueness of his creative path and from the severe problems it brought about. To them, he reacted following his own nature, eventually shaping the peculiar personality of Apple. The following paper will try to clarify such notion.

  15. Sedimentation measurements of apple pectins molecular weight

    International Nuclear Information System (INIS)

    The article is described the sedimentation measurements of a number of apple pectin's molecular weight using only sedimentation diagrams through computer program. This approach has good readability and some advantages over others

  16. Production of apple snail for space diet

    Science.gov (United States)

    Yamashita, Masamichi; Motoki, Shigeru; Space Agriculture Task Force, J.; Katayama, Naomi

    For food production in space at recycling bio-elements under closed environment, appropriate organisms should be chosen to drive the closed materials recycle loop. We propose a combination of green algae, photosynthetic protozoa, and aquatic plants such as Wolffia spp., for the primary producer fixing solar energy to chemical form in biomass, and apple snail, Pomacea bridgesii, which converts this biomass to animal meat. Because of high proliferation rate of green algae or protozoa compared to higher plants, and direct conversion of them to apple snail, the efficiency of food production in this combination is high, in terms of energy usage, space for rearing, and yield of edible biomass. Furthermore, green algae and apple snail can form a closed ecological system with exchanging bio-elements between two member, i.e. excreta of snail turn to fertilizer of algae, and grown algae become feed for snail. Since apple snail stays in water or on wet substrate, control of rearing is easy to make. Mass production technology of apple snail has been well established to utilize it as human food. Nutrients of apple snail are also listed in the standard tables of food composition in Japan. Nutrients for 100 g of apple snail canned in brine are energy 340 kJ, protein 16.5 g, lipid 1.0 g, cholesterol 240 mg, carbohydrate 0.8 g, Ca 400 mg, Fe 3.9 mg, Zn 1.5 mg. It is rich in minerals, especially Ca and Fe. Vitamin contents are quite low, but K 0.005 mg, B2 0.09 mg, B12 0.0006 mg, folate 0.001 mg, and E 0.6 mg. The amino acid score of apple snail could not be found in literature. Overall, apple snail provides rich protein and animal lipid such as cholesterol. It could be a good source of minerals. However, it does not give enough vitamin D and B12 , which are supposed to be supplemented by animal origin foods. In terms of acceptance in food culture, escargot is a gourmet menu in French dishes, and six to ten snail, roughly 50 g, are served for one person. Apple snail reaches to 30 g

  17. Determination of daminozide in apples and apple leaves by liquid chromatography-mass spectrometry

    NARCIS (Netherlands)

    Mol, H.G.J.; Dam, R.C.J. van; Vreeken, R.J.; Steijger, O.M.

    1999-01-01

    A straightforward and efficient method was developed for the determination of intact daminozide in apples and apple leaves. After extraction with methanol and a clean-up step using a graphitized carbon cartridge, the extract was analysed by ion-trap liquid chromatography-tandem mass spectrometry (LC

  18. Constructing and Using an Apple IIe Computer AppleWorks Document Library.

    Science.gov (United States)

    Schlenker, Richard M.

    This document presents a step-by-step procedure for setting up a document library of personal word processing, database, and spreadsheet files using the Apple IIe computer and the AppleWorks subprogram database. This library, which can serve both as a running record of files created and as a means for easy retrieval, uses 10 fields or categories…

  19. Constructing AppleWorks Word Processing Files for the Apple IIe Computer.

    Science.gov (United States)

    Schlenker, Richard M.

    This step-by-step guide to constructing word processing files using the AppleWorks software on the Apple IIe computer covers (1) loading the program; (2) adding files to the desktop; (3) selecting the word processor option; (4) naming the file; (5) setting tabs; (6) selecting print options; and (7) saving the file. Sixteen sample screen displays…

  20. Constructing AppleWorks Data Base Files for the Apple IIe Computer.

    Science.gov (United States)

    Schlenker, Richard M.

    This step-by-step guide to creating database files using the AppleWorks software on the Apple IIe computer covers (1) loading the program; (2) adding files to the desktop; (3) selecting the database option; (4) naming the file; (5) naming categories or fields; (6) inserting data; (7) changing database file formats; (8) altering the file layout;…

  1. Printing AppleWorks Data Base Files with the Apple IIe Computer.

    Science.gov (United States)

    Schlenker, Richard M.

    This step-by-step guide to printing AppleWorks database files using the Apple IIe computer covers (1) naming the report; (2) selecting a printer; and (3) printing the report for both the labels and the tables formats. Twenty-one sample screen displays which illustrate the steps and examples of the printed reports are included. (MES)

  2. Field testing of potential new compounds for apple scab control in organic apple production.

    OpenAIRE

    Pedersen, Seniorscientist Hanne Lindhard; Paaske, Klaus; Bengtsson, Marianne; Hockenhull, John

    2006-01-01

    Abstract - Improved prevention and control of apple scab caused by Venturia inaequalis without the use of copper is the aim of this project. Twenty potential new compounds were tested in a field trial in a single season. One compound was found to reduce apple scab infections significantly. The research continues in 2006 and 2007.

  3. Effects of apples and specific apple components on the cecal environment of conventional rats: role of apple pectin

    Directory of Open Access Journals (Sweden)

    Markowski Jaroslaw

    2010-01-01

    Full Text Available Abstract Background Our study was part of the large European project ISAFRUIT aiming to reveal the biological explanations for the epidemiologically well-established health effects of fruits. The objective was to identify effects of apple and apple product consumption on the composition of the cecal microbial community in rats, as well as on a number of cecal parameters, which may be influenced by a changed microbiota. Results Principal Component Analysis (PCA of cecal microbiota profiles obtained by PCR-DGGE targeting bacterial 16S rRNA genes showed an effect of whole apples in a long-term feeding study (14 weeks, while no effects of apple juice, purée or pomace on microbial composition in cecum were observed. Administration of either 0.33 or 3.3% apple pectin in the diet resulted in considerable changes in the DGGE profiles. A 2-fold increase in the activity of beta-glucuronidase was observed in animals fed with pectin (7% in the diet for four weeks, as compared to control animals (P Bacteroidetes, whereas bands that became more prominent represented mainly Gram-positive anaerobic rods belonging to the phylum Firmicutes, and specific species belonging to the Clostridium Cluster XIVa. Quantitative real-time PCR confirmed a lower amount of given Bacteroidetes species in the pectin-fed rats as well as in the apple-fed rats in the four-week study (P Clostridium coccoides (belonging to Cluster XIVa, as well as of genes encoding butyryl-coenzyme A CoA transferase, which is involved in butyrate production, was detected by quantitative PCR in fecal samples from the pectin-fed animals. Conclusions Our findings show that consumption of apple pectin (7% in the diet increases the population of butyrate- and β-glucuronidase producing Clostridiales, and decreases the population of specific species within the Bacteroidetes group in the rat gut. Similar changes were not caused by consumption of whole apples, apple juice, purée or pomace.

  4. Cisgenic apple trees; development, characterization, and performance.

    Science.gov (United States)

    Krens, Frans A; Schaart, Jan G; van der Burgh, Aranka M; Tinnenbroek-Capel, Iris E M; Groenwold, Remmelt; Kodde, Linda P; Broggini, Giovanni A L; Gessler, Cesare; Schouten, Henk J

    2015-01-01

    Two methods were developed for the generation of cisgenic apples. Both have been successfully applied producing trees. The first method avoids the use of any foreign selectable marker genes; only the gene-of-interest is integrated between the T-DNA border sequences. The second method makes use of recombinase-based marker excision. For the first method we used the MdMYB10 gene from a red-fleshed apple coding for a transcription factor involved in regulating anthocyanin biosynthesis. Red plantlets were obtained and presence of the cisgene was confirmed. Plantlets were grafted and grown in a greenhouse. After 3 years, the first flowers appeared, showing red petals. Pollination led to production of red-fleshed cisgenic apples. The second method used the pM(arker)F(ree) vector system, introducing the scab resistance gene Rvi6, derived from apple. Agrobacterium-mediated transformation, followed by selection on kanamycin, produced genetically modified apple lines. Next, leaves from in vitro material were treated to activate the recombinase leading to excision of selection genes. Subsequently, the leaf explants were subjected to negative selection for marker-free plantlets by inducing regeneration on medium containing 5-fluorocytosine. After verification of the marker-free nature, the obtained plants were grafted onto rootstocks. Young trees from four cisgenic lines and one intragenic line, all containing Rvi6, were planted in an orchard. Appropriate controls were incorporated in this trial. We scored scab incidence for three consecutive years on leaves after inoculations with Rvi6-avirulent strains. One cisgenic line and the intragenic line performed as well as the resistant control. In 2014 trees started to overcome their juvenile character and formed flowers and fruits. The first results of scoring scab symptoms on apple fruits were obtained. Apple fruits from susceptible controls showed scab symptoms, while fruits from cisgenic and intragenic lines were free of scab

  5. Cisgenic apple trees; development, characterization and performance

    Directory of Open Access Journals (Sweden)

    Frans A. Krens

    2015-04-01

    Full Text Available Two methods were developed for the generation of cisgenic apples. Both have been successfully applied producing trees. The first method avoids the use of any foreign selectable marker genes; only the gene-of-interest is integrated between the T-DNA border sequences. The second method makes use of recombinase-based marker excision. For the first method we used the MdMYB10 gene from a red-fleshed apple coding for a transcription factor involved in regulating anthocyanin biosynthesis. Red plantlets were obtained and presence of the cisgene was confirmed. Plantlets were grafted and grown in a greenhouse. After three years, the first flowers appeared, showing red petals. Pollination led to production of red-fleshed cisgenic apples. The second method used the pM(arkerF(ree vector system, introducing the scab resistance gene Rvi6, derived from apple. Agrobacterium-mediated transformation, followed by selection on kanamycin, produced genetically modified apple lines. Next, leaves from in vitro material were treated to activate the recombinase leading to excision of selection genes. Subsequently, the leaf explants were subjected to negative selection for marker-free plantlets by inducing regeneration on medium containing 5-fluorocytosine. After verification of the marker-free nature, the obtained plants were grafted onto rootstocks. Young trees from four cisgenic lines and one intragenic line, all containing Rvi6, were planted in an orchard. Appropriate controls were incorporated in this trial. We scored scab incidence for three consecutive years on leaves after inoculations with Rvi6-avirulent strains. One cisgenic line and the intragenic line performed as well as the resistant control. In 2014 trees started to overcome their juvenile character and formed flowers and fruits. The first results of scoring scab symptoms on apple fruits were obtained. Apple fruits from susceptible controls showed scab symptoms, while fruits from cisgenic and intragenic

  6. 40 CFR 407.10 - Applicability; description of the apple juice subcategory.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Applicability; description of the apple... SOURCE CATEGORY Apple Juice Subcategory § 407.10 Applicability; description of the apple juice... apples into apple juice or apple cider. When a plant is subject to effluent limitations covering...

  7. 40 CFR 407.20 - Applicability; description of the apple products subcategory.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Applicability; description of the apple... SOURCE CATEGORY Apple Products Subcategory § 407.20 Applicability; description of the apple products... apples into apple products. The processing of apples into caustic peeled or dehydrated products...

  8. Application of molecular markers in apple breeding

    Directory of Open Access Journals (Sweden)

    Marić Slađana

    2010-01-01

    Full Text Available Apple is economically the most important species of genus Malus Miller. In respect of production, trade and consumption, it ranks first among deciduous fruit and third on a global scale among all fruit species. Apple breeding is carried out on a large scale in several scientific institutes throughout the world. Due to this activity, apple is a fruit species with the highest number of described monogenic traits; 76 genes, encoding morphological traits, pest and disease resistance, as well as 69 genes encoding enzymes. The development of molecular markers (RFLPs, AFLPs, SCARs and SSRs has allowed the mapping of the apple genome and the development of several saturated genetic maps, to which genes controlling important traits are assigned. Markers flanking these genes not only play an important role in selecting parental combinations and seedlings with positive traits, but they are also particularly important in detecting recessive traits, such as seedless fruit. In addition they enable pre-selection for polygenic quantitative traits. In recent years, particular attention has been paid to biochemical and physiological processes involved in the pathway of important traits e.g., ripening and the storage capability of apple fruit.

  9. Biotechnology and apple breeding in Japan.

    Science.gov (United States)

    Igarashi, Megumi; Hatsuyama, Yoshimichi; Harada, Takeo; Fukasawa-Akada, Tomoko

    2016-01-01

    Apple is a fruit crop of significant economic importance, and breeders world wide continue to develop novel cultivars with improved characteristics. The lengthy juvenile period and the large field space required to grow apple populations have imposed major limitations on breeding. Various molecular biological techniques have been employed to make apple breeding easier. Transgenic technology has facilitated the development of apples with resistance to fungal or bacterial diseases, improved fruit quality, or root stocks with better rooting or dwarfing ability. DNA markers for disease resistance (scab, powdery mildew, fire-blight, Alternaria blotch) and fruit skin color have also been developed, and marker-assisted selection (MAS) has been employed in breeding programs. In the last decade, genomic sequences and chromosome maps of various cultivars have become available, allowing the development of large SNP arrays, enabling efficient QTL mapping and genomic selection (GS). In recent years, new technologies for genetic improvement, such as trans-grafting, virus vectors, and genome-editing, have emerged. Using these techniques, no foreign genes are present in the final product, and some of them show considerable promise for application to apple breeding. PMID:27069388

  10. Carbon isotope analysis in apple nectar beverages

    Directory of Open Access Journals (Sweden)

    Ricardo Figueira

    2013-03-01

    Full Text Available The aims of this study were to use the isotope analysis method to quantify the carbon of C3 photosynthetic cycle in commercial apple nectars and to determine the legal limit to identify the beverages that do not conform to the safety standards established by the Brazilian Ministry of Agriculture, Livestock and Food Supply. These beverages (apple nectars were produced in the laboratory according to the Brazilian legislation. Adulterated nectars were also produced with an amount of pulp juice below the permitted threshold limit value. The δ13C values of the apple nectars and their fractions (pulp and purified sugar were measured to quantify the C3 source percentage. In order to demonstrate the existence of adulteration, the values found were compared to the limit values established by the Brazilian Law. All commercial apple nectars analyzed were within the legal limits, which enabled to identify the nectars that were in conformity with the Brazilian Law. The isotopic methodology developed proved efficient to quantify the carbon of C3 origin in commercial apple nectars.

  11. Goldstone-Apple Valley Radio Telescope System Theory of Operation

    Science.gov (United States)

    Stephan, George R.

    1997-01-01

    The purpose of this learning module is to enable learners to describe how the Goldstone-Apple Valley Radio Telescope (GAVRT) system functions in support of Apple Valley Science and Technology Center's (AVSTC) client schools' radio astronomy activities.

  12. Consumer Preferences: A Guide to Connecticut Apple Marketing

    OpenAIRE

    Leonard, Robert L.; Wadsworth, James J.

    1989-01-01

    The primary objectives of this research are: (1) to identify the preferences of Connecticut apple consumers regarding product attributes and market characteristics; (2) to assess the impact of Connecticut promotion programs on consumer preference for Connecticut grown apples, and (3) to identify opportunities for more effective marketing of Connecticut grown apples. Information was obtained by interviewing a total of 374 persons purchasing apples at farm stores, pick-your-own orchards, superm...

  13. Towards durabale resistance to apple scab using cisgenes

    OpenAIRE

    Joshi, S. G.

    2010-01-01

    Apple (Malus x domestica) is one of the important fruit crops of the world. It is mainly cultivated in temperate regions. Apple fruit contains many health beneficial compounds which may play an important role in reducing cancer cell proliferation and lowering the level of cholesterol. Apple production can suffer from several pests and diseases and among them scab is very important. Apple scab is a fungal disease caused by Venturia inaequalis. The pathogen is a facultative saprophyte that grow...

  14. Consumer acceptance of irradiated food products: an apple marketing study

    International Nuclear Information System (INIS)

    This study was exploratory in nature, with emphasis on initial purchases and not repeat purchases or long-term loyalties to either irradiated or non-irradiated produce. The investigation involved the actual sale of irradiated and non-irradiated apples to consumers. Limited information about the process was provided, and apples were sold at roadside stands. Prices for the irradiated apples were varied while the price for the non-irradiated apples was held constant. Of these 228 West-Central Missouri shoppers, 101 (44%) bought no irradiated apples, 86 (38%) bought only irradiated apples, and 41 (18%) bought some of both types, Results of probit regressions indicated three significant independent variables. There was an inverse relationship between the price of irradiated apples and the probability of purchasing irradiated apples. There was a positive relationship between the purchasers’ educational level and the probability of purchasing irradiated apples. Predicted probabilities for belonging to categories in probit models were computed. Depending on particular equation specification, correctly placed were approximately 70 percent of the purchasers of the two categories--bought only non-irradiated apples, or bought some of both irradiated and non-irradiated apples or only irradiated apples. This study suggests that consumers may be interested in food irradiation as a possible alternative or supplement to current preservation techniques

  15. Apple'i kasum purustas ootusi / Virge Lahe

    Index Scriptorium Estoniae

    Lahe, Virge

    2007-01-01

    Ameerika arvutitootja Apple'i suur kasum tagas head eeldused tarkvarafirma mobiiltelefoni iPhone'i eduks. Kommenteerib Sander Paas. Diagramm: Apple'i aktsia rallis majandustulemuste peale üle 10 protsendi. Tabel: Apple'i kasum segmentide kaupa mln dollarites

  16. The Microwave Concentrator Design and Study on Concentrating Apple Juice

    OpenAIRE

    Geng Yuefeng; Ge Xinfeng

    2014-01-01

    Microwave concentrating device on fresh apple juice is designed in order to increase concentrating fresh apple juice efficiency and microwave concentrating process on fresh apple juice were studied. The designed microwave concentrator contains microwave generator, dehumidification system, electrical cabinet, parabolic waveguide, control system, microwave leakage inhibited mechanism and other components. The concentrating experiment is carried by the designed concentrator, from the setting-to-...

  17. 7 CFR 457.158 - Apple crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Apple crop insurance provisions. 457.158 Section 457... CORPORATION, DEPARTMENT OF AGRICULTURE COMMON CROP INSURANCE REGULATIONS § 457.158 Apple crop insurance provisions. The Apple Crop Insurance Provisions for the 2005 and succeeding crop years are as follows:...

  18. Towards durabale resistance to apple scab using cisgenes

    NARCIS (Netherlands)

    Joshi, S.G.

    2010-01-01

    Apple (Malus x domestica) is one of the important fruit crops of the world. It is mainly cultivated in temperate regions. Apple fruit contains many health beneficial compounds which may play an important role in reducing cancer cell proliferation and lowering the level of cholesterol. Apple product

  19. Stable-isotope composition of the water of apple juice

    International Nuclear Information System (INIS)

    By deuterium and oxygen 18 analysis, it was shown that apples' water is enriched in heavier isotopes as compared to rain water. The isotopic composition of the water of reconstituted apple juice is closed to the isotopic content of the rain water used for dilution. Thus, deuterium and oxyden 18 analysis allows a good analytical distinction between natural apple juice and reconstituted juices

  20. The Venturia Apple Pathosystem: Pathogenicity Mechanisms and Plant Defense Responses

    OpenAIRE

    Gopaljee Jha; Karnika Thakur; Priyanka Thakur

    2009-01-01

    Venturia inaequalis is the causal agent of apple scab, a devastating disease of apple. We outline several unique features of this pathogen which are useful for molecular genetics studies intended to understand plant-pathogen interactions. The pathogenicity mechanisms of the pathogen and overview of apple defense responses, monogenic and polygenic resistance, and their utilization in scab resistance breeding programs are also reviewed.

  1. Simultaneous ingestion of high-methoxy pectin from apple can enhance absorption of quercetin in human subjects.

    Science.gov (United States)

    Nishijima, Tomohiko; Takida, Yoshiki; Saito, Yasuo; Ikeda, Takayuki; Iwai, Kunihisa

    2015-05-28

    Chronic ingestion of apple pectin has been shown to increase the absorption of quercetin in rats. The present study was designed to elucidate whether the simultaneous ingestion of quercetin with apple pectin could enhance the absorption of quercetin in humans, and the effects of dose dependency and degree of pectin methylation on quercetin absorption were also investigated. Healthy volunteers (n 19) received 200 ml of 0.5 mg/ml of quercetin drinks with or without 10 mg/ml of pectin each in a randomised cross-over design study with over 1-week intervals; urine samples from all the subjects were collected within 24 h after ingestion of the test drinks, and urinary deconjugated quercetin and its metabolites were determined using HPLC. The sum of urinary quercetin and its metabolites excreted was increased by 2.5-fold by the simultaneous ingestion of pectin. The metabolism of methylated quercetin (isorhamnetin and tamarixetin) was not affected by pectin ingestion. In six volunteers, who received quercetin drinks containing 0, 3 and 10 mg/ml of pectin, the sum of urinary quercetin and its metabolites excreted also increased in a pectin dose-dependent manner. Furthermore, the simultaneous ingestion of quercetin with low-methoxy and high-methoxy pectin, respectively, increased the sum of urinary excretion of quercetin and its metabolites by 1.69-fold and significantly by 2.13-fold compared with the ingestion of quercetin without pectin. These results elucidated that apple pectin immediately enhanced quercetin absorption in human subjects, and that its enhancing effect was dependent on the dose and degree of pectin methylation. The results also suggested that the viscosity of pectin may play a role in the enhancement of quercetin absorption. PMID:25865751

  2. Proteome Analysis for Defense Response of Apple Leaves Induced by Alternaria Blotch ,Alternaria alternata Apple Pathotype

    Institute of Scientific and Technical Information of China (English)

    Caixia Zhang; Zhuang Li; Liyi Zhang; Yi Tian; Guodong Kang; Ying Chen; Peihua Cong

    2012-01-01

    Apple (Ma/us domestica) is considered a model fruit plant owing to its world-wide economic importance,and a large number of cultivars dominate world fruit production.To date,the cultivation of apple has been limited by many kinds of fungal diseases,and among the many fungal diseases affecting apple trees,Alternaria blotch,the disease caused by Alternaria alternata apple pathotype,is spreading worldwide and results in severe negative effect on apple production.Currently,management of a fungal disease such as Alternaria blotch mainly through traditional chemical control agents instead of using resistant cultivars.As in apple,the molecular mechanism of disease resistance against A.altemata apple pathotype has not been illustrated clearly,although most of the genes have been identified in apple,little is known about the biological roles for each protein in that plant.Considering that apple Altemaria blotch is the usual diseases influencing apple production,further studies about the interaction mechanism between the pathogen and its host,particularly some host self-defense response mechanisms which closely related to the anti-disease properties of apple should be performed.In this work we aimed to generate fundamental insights into the plant' s defense responses to infection with Altemaria blotch using a proteomics approach,to aid in the development and breeding of high-quality disease-resistant apple varieties.One seedlings which derived from the cross of ‘Huacui’ and ‘Golden Delicious’,with highly resistant,were chosen to be hosts for this study.The sample leaves were inoculated with spore suspension of A.altemata apple pathotype,and the control samples were treated with water instead of inoculums.Following inoculation,the samples were harvested 48 h after inoculation.The whole experiment was repeated three times in order to get a reliable result.The total soluble protein extracts prepared using modified phenol-precipitation procedures.Total protein extracts

  3. Marketingové strategie firmy APPLE

    OpenAIRE

    Preněk, Tomáš

    2008-01-01

    The main goal of this thesis is a definition of marketing goals and strategies of the Apple company on the basis of the complex positional analysis, whose output is the SWOT analysis. For purpose of using theoretical knowledge in praxis one part is devoted to the marketing instruments, which are used by the analyzed company. This part leads to analysis of the company's marketing mix of one of the product made by Apple. This thesis includes also results of the market research which was focused...

  4. Take control of Apple Mail in Leopard

    CERN Document Server

    Kissell, Joe

    2009-01-01

    Go under the hood with new (and old) features in Apple Mail in Leopard! Are you using Apple Mail in Leopard effectively? In this book, completely updated from its previous Panther and Tiger editions, author Joe Kissell provides comprehensive guidance, with a focus on new and updated features. You'll learn how to use and customize the Mail window, control the size and styling of incoming messages, and make rules to move messages into different mailboxes automatically. The book covers outgoing mail, showing you smart ways to address messages, send attachments, and send HTML-based messages. Bu

  5. APPL proteins FRET at the BAR: direct observation of APPL1 and APPL2 BAR domain-mediated interactions on cell membranes using FRET microscopy.

    Directory of Open Access Journals (Sweden)

    Heidi J Chial

    Full Text Available BACKGROUND: Human APPL1 and APPL2 are homologous RAB5 effectors whose binding partners include a diverse set of transmembrane receptors, signaling proteins, and phosphoinositides. APPL proteins associate dynamically with endosomal membranes and are proposed to function in endosome-mediated signaling pathways linking the cell surface to the cell nucleus. APPL proteins contain an N-terminal Bin/Amphiphysin/Rvs (BAR domain, a central pleckstrin homology (PH domain, and a C-terminal phosphotyrosine binding (PTB domain. Previous structural and biochemical studies have shown that the APPL BAR domains mediate homotypic and heterotypic APPL-APPL interactions and that the APPL1 BAR domain forms crescent-shaped dimers. Although previous studies have shown that APPL minimal BAR domains associate with curved cell membranes, direct interaction between APPL BAR domains on cell membranes in vivo has not been reported. METHODOLOGY: Herein, we used a laser-scanning confocal microscope equipped with a spectral detector to carry out fluorescence resonance energy transfer (FRET experiments with cyan fluorescent protein/yellow fluorescent protein (CFP/YFP FRET donor/acceptor pairs to examine interactions between APPL minimal BAR domains at the subcellular level. This comprehensive approach enabled us to evaluate FRET levels in a single cell using three methods: sensitized emission, standard acceptor photobleaching, and sequential acceptor photobleaching. We also analyzed emission spectra to address an outstanding controversy regarding the use of CFP donor/YFP acceptor pairs in FRET acceptor photobleaching experiments, based on reports that photobleaching of YFP converts it into a CFP-like species. CONCLUSIONS: All three methods consistently showed significant FRET between APPL minimal BAR domain FRET pairs, indicating that they interact directly in a homotypic (i.e., APPL1-APPL1 and APPL2-APPL2 and heterotypic (i.e., APPL1-APPL2 manner on curved cell membranes

  6. High-pressure washing treatments to remove obscure mealybug (Homoptera: Pseudococcidae) and lightbrown apple moth (Lepidoptera: Tortricidae) from harvested apples.

    Science.gov (United States)

    Whiting, D C; Hoy, L E; Maindonald, J H; Connolly, P G; McDonald, R M

    1998-12-01

    Mixed life stages of obscure mealybug, Pseudococcus viburni (Signoret), and late 1st-instar or early 2nd-instar lightbrown apple moth, Epiphyas postvittana (Walker), on 'Royal Gala' apples (Malus domestica Borkhausen) were exposed to standard packhouse processing with and without addition of high-pressure apple washer treatments. Insect removal and mortality were assessed. After standard packhouse processing approximately 60% of P. viburni remained on their host apples. The 2 high-pressure apple washer treatments (500 and 800 psi at 2.0 rods/s) were equally effective and significantly reduced the number of P. viburni on apples compared with the packhouse control. High-pressure apple washer removal by location decreased in the following order: calyx cavity outside the sepals > cheek approximately stem cavity > or = calyx beneath sepals. About half of the E. postvittana larvae infesting apples was removed by standard packhouse processing. Removal rates were similar for all locations on open-calyxed apples. However, no removal occurred from the calyx beneath the sepals if the apple calyx was closed. All 4 high-pressure apple washer treatments tested (500 and 800 psi at 1.0 and 2.0 rods/s) halved the number of larvae on the apple exterior relative to the packhouse control. The pattern of removal for larvae on open-calyxed apples was calyx outside sepals approximately stem cavity > calyx beneath the sepals approximately cheek. A similar pattern was evident for larvae on closed-calyxed apples, except insects beneath the sepals evaded removal. The persistence of insects on the apple cheek reflects the high proportion of larvae inside tunnels in this location compared with other apple locations. Removal of internally positioned insects was much lower than that of externally positioned insects. PMID:9887685

  7. Metabolomics for Secondary Metabolite Research

    Directory of Open Access Journals (Sweden)

    Eriko Takano

    2013-11-01

    Full Text Available Metabolomics, the global characterization of metabolite profiles, is becoming an increasingly powerful tool for research on secondary metabolite discovery and production. In this review we discuss examples of recent technological advances and biological applications of metabolomics in the search for chemical novelty and the engineered production of bioactive secondary metabolites.

  8. Towards sustainable intensiifcation of apple production in China-Yield gaps and nutrient use efifciency in apple farming systems

    Institute of Scientific and Technical Information of China (English)

    WANG Na; Joost Wolf; ZHANG Fu-suo

    2016-01-01

    China is in a dominant position in apple production globaly with both the largest apple growing area and the largest export of fresh apple fruits. However, the annual productivity of China’s apple is signiifcantly lower than that of other dominant apple producing countries. In addition, apple production is based on excessive application of chemical fertilizers and the nutrient use efifciency (especialy nitrogen) is therefore low and the nutrient emissions to the environment are high. Apple production in China is considerably contributes to farmers’ incomes and is important as export product. There is an urgent need to enhance apple productivity and improve nutrient use efifciencies in intensive apple production systems in the country. These can be attained by improved understanding of production potential, yield gaps, nutrient use and best management in apple orchards. To the end, priorities in research on apple production systems and required political support are described which may lead to more sustainable and environmental-friendly intensiifcation of apple production in China.

  9. Developing Simple Financial Records Using the AppleWorks Spreadsheet Subprogram, Apple IIe or GS Computers, and a Duodisk Drive.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for developing simple financial records using the AppleWorks spreadsheet subprogram with an Apple IIe or Apple IIGS Computer which has a Duodisk or two disk drives. The manual provides step-by-step directions, and includes 34 figures depicting the computer screen at the various stages of the spreadsheet…

  10. Developing Simple Budgets Using the AppleWorks Spreadsheet Subprogram, Apple IIe or GS Computers, and a Single Disk Drive.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for developing spreadsheet files in the AppleWorks program using an Apple IIe or Apple IIGS Computer with a single disk drive. The manual provides step-by-step directions, and includes 36 figures depicting the computer screen at the various stages of the spreadsheet development sequence. (EW)

  11. Developing Inventory Records Using the AppleWorks Data Base Subprogram and Apple IIe or GS Computers.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for developing inventory records in the AppleWorks program using an Apple IIe or Apple IIGS Computer with Duodisk or two disk drives and an 80-column card. The manual provides step-by-step directions, and includes 17 figures depicting the computer screen at the various stages of the inventory records…

  12. Building Parts Inventory Files Using the AppleWorks Data Base Subprogram and Apple IIe or GS Computers.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for building database files using the AppleWorks program with an Apple IIe or Apple IIGS Computer with Duodisk or two disk drives and an 80-column card. The manual provides step-by-step directions, and includes 25 figures depicting the computer screen at the various stages of the database file building…

  13. Setting Up Letters Using the AppleWorks Word Processor Subprogram and Apple IIe or GS Computers.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for setting up letter word processing files using the AppleWorks program with an Apple IIe or Apple IIGS computer which has a Duodisk or two disk drives and an 80-column card. The manual provides step-by-step directions, and includes 16 figures depicting the computer screen at the various stages of word…

  14. Biosynthesis of pectinolytic enzymes by Aspergillus on apple pulp

    OpenAIRE

    Mojsov, Kiro; Petreska, Meri; Ziberoski, Jugoslav

    2012-01-01

    The aim of this work was to develop a low cost process for apple pulp utilization. The apple pulp combined with corn flour and simple mineral salts by submerged production of pectinolytic enzymes by the fungus Aspergillus. Different concentration on apple pulp and different pH initial on the bases were studied, and all other process parameters were same. Results of different concentration on apple pulp gived maximal endo- PG with 1% apple pulp, during from 96 h, and the growth of the micr...

  15. Bioanalytical characterization of apple juice from 88 grafted and nongrafted apple varieties grown in Upper Austria.

    Science.gov (United States)

    Lanzerstorfer, Peter; Wruss, Jürgen; Huemer, Stefan; Steininger, Andrea; Müller, Ulrike; Himmelsbach, Markus; Borgmann, Daniela; Winkler, Stephan; Höglinger, Otmar; Weghuber, Julian

    2014-02-01

    The compositional characteristics of untreated pure juice prepared from 88 apple varieties grown in the region of Eferding/Upper Austria were determined. Many of the analyzed varieties are noncommercial, old varieties not present in the market. The aim of the study was to quantitate the mineral, phosphate, trace elements, and polyphenolic content in order to identify varieties that are of particular interest for a wider distribution. Great variations among the investigated varieties could be found. This holds especially true for the total polyphenolic content (TPC) ranging from 103.2 to 2,275.6 mg/L. A clear dependence of the antioxidant capacity on the TPC levels was detected. Bioinformatics was employed to find specific interrelationships, such as Mg²⁺/Mn²⁺ and PO₄³⁻/K⁺, between the analyzed bio- and phytochemical parameters. Furthermore, special attention was drawn on putative effects of grafting on the phytochemical composition of apple varieties. By grafting 27 different apple varieties on two trees grown close to each other, it could be shown that the apple fruits remain their characteristic phytochemical composition. Finally, apple juice prepared from selected varieties was further characterized by additional biochemical analysis including cytotoxicity, epidermal growth factor receptor (EGFR) inhibition, and α-amylase activity tests. Cytotoxicity and inhibition of EGFR activation were found to be dependent on the TPC, while α-amylase activity was reduced by the apple juices independent of the presence of polyphenolic substances. Taken together selected apple varieties investigated within this study might serve as preferable sources for the development of apple-based food with a strong focus on health beneficial effects. PMID:24410208

  16. Apple and pear rootstock research in Lithuania

    OpenAIRE

    Kviklys, Darius

    2006-01-01

    The paper presents ongoing apple and pear rootstock trials at the Lithuanian Institute of Horticulture. Rootstock research projects are established in following directions: rootstock and location interaction (Baltic fruit rootstock studies where Byelorussian, Estonian, Latvian, Lithuanian and Polish research institutions are involved); budding high effect on rootstock performance; interstock trials; rootstock effect on fruit quality, ripening time and fruit storage; rootstock and tree trainin...

  17. Cisgenic apple trees; development, characterization, and performance

    NARCIS (Netherlands)

    Krens, F.A.; Schaart, J.G.; Burgh, van der A.M.; Tinnenbroek-Capel, I.E.M.; Groenwold, R.; Kodde, L.P.; Broggini, G.A.L.; Gessler, C.; Schouten, H.J.

    2015-01-01

    Two methods were developed for the generation of cisgenic apples. Both have been successfully applied producing trees. The first method avoids the use of any foreign selectable marker genes; only the gene-of-interest is integrated between the T-DNA border sequences. The second method makes use of re

  18. POTENTIAL EXPOSURE OF APPLE THINNERS TO PHOSALONE

    Science.gov (United States)

    Apple thinning results in considerable human contact with foliage, fruit, and limbs. This work often occurs during that portion of the growing season when trees are most frequently being sprayed for insect conrol. Therefore, of all workers exposed to pesticide residues upon reent...

  19. Questions and Answers: Apple Juice and Arsenic

    Science.gov (United States)

    ... and monomethylarsonic acid (MMA), may also be a health concern. Are apple and other fruit juices safe to drink? The FDA has been ... this, the FDA is considering how any possible health risk from these two forms of ... arsenic in fruit juice? The FDA has proposed an “action level” ...

  20. Statement on the identity of apple snails

    DEFF Research Database (Denmark)

    Baker, R.; Candresse, T.; Dormannsné Simon, E.; Gilioli, G.; Grégoire, J.-C.; Jeger, M. J.; Karadjova, O. E.; Lövei, G.; Makowski, D.; Manceau, C.; Navajas, M.; Porta Puglia, A.; Rafoss, T.; Rossi, V.; Schans, J.; Schrader, G.; Urek, G.; van Lenteren, J. C.; Vloutoglou, I; Winter, S.; Zlotina, M.

    2012-01-01

    Following a request by the European Commission, EFSA’s Panel on Plant Health was asked to deliver a statement to clarify the current scientific knowledge regarding the identity of the apple snails in the context of the evaluation of the pest risk analysis prepared by the Spanish Ministry of Envir...

  1. Steven jobs and his apple computer

    Institute of Scientific and Technical Information of China (English)

    陈春枝

    2002-01-01

    The designer(设计者)of apple computer,steven jobs,was not quite successful(成功的)in his early years.he was not among the best students at school,and from time to time he got into truble with either his schoolmates or his teachers.but he was full of new ideas,

  2. A global conservation strategy for apple

    Science.gov (United States)

    Apple (Malus x domestica) production is #17 for agricultural products both in the U.S. and world with a value of more than $31 billion worldwide. This important perennial crop is expensive to produce, with high costs for land, labor and inputs. The industry is dominated by a relatively few number of...

  3. Making Apple Computers Accessible to Blind Children.

    Science.gov (United States)

    Renouf, Andrew; Phillips, Susan

    The study examined the feasibility of adapting commercially available educational software to a speech synthesizer compatible with the Apple II for use with 15 visually impaired students 8 to 12 years old. Ss were pre-tested on measures of auditory discrimination, computer literacy, keyboard proficiency, spelling, and language. Ss then received…

  4. Mom, Apple Pie, and the American Dream.

    Science.gov (United States)

    Grambs, Jean Dresden

    1982-01-01

    Discusses how the mom-and-apple-pie facet of the American dream no longer seems to be working. Ways to redefine that dream so that women, men, children, and families are comfortable with each other and are able to develop a mutual dependence which also allows for mutual independence are examined. (RM)

  5. Degradation of apple fruit xyloglucan by endoglucanase.

    NARCIS (Netherlands)

    Vincken, J.P.; Beldman, G.; Niessen, W.M.A.; Voragen, A.G.J.

    1996-01-01

    A purified, alkali-extractable apple fruit xyloglucan (APfxg) was treated by endoglucanase (endoIV) from Trichoderma viride. The degradation products were fractionated by size-exclusion chromatography on BioGel P-2; the pentamer to dodecamer fractions were further fractionated by semi-preparative hi

  6. Optical Properties of Bruised Apple Tissue

    Science.gov (United States)

    Understanding the optical properties of apple tissue, especially bruised tissue, can help us prevent or mitigate bruise occurrence during harvest and postharvest operations, and develop an effective method for detecting bruises during sorting and grading. This research was aimed at determining the o...

  7. Research on the Influencing Factors of China Apple Juice Trade

    Directory of Open Access Journals (Sweden)

    Du Juan

    2013-06-01

    Full Text Available China is the first country in the world in which apple juice is produced and exited and the main producing area is concentrated in the north of China. Some domestic companies which export apple juice are founded. China’s apple juice, mainly exported to USA, Japan and the Europe, has a strong international competitiveness. However, due to the breed and raw material, Chinese apple juice export faces some challenge, like the loss happening in the transport process. The objective of this study is to research China's apple juice export situation and problem using the comparative analysis method. To cut down the loss, this study is trying to offer a relative scientific research for fruit juice industry by analyzing how temperature and concentration influence on thermal conductivity of apple juice, affecting the whole juice industry. It is with great significance to solve the realistic problems and promote China apple juice industry and its international trade.

  8. Facilitated cellular uptake and suppression of inducible nitric oxide synthase by a metabolite of maritime pine bark extract (Pycnogenol).

    Science.gov (United States)

    Uhlenhut, Klaus; Högger, Petra

    2012-07-15

    Many natural products exhibit anti-inflammatory activity by suppressing excessive nitric oxide (NO) production by inducible NO synthase (iNOS). The maritime pine bark extract Pycnogenol has been formerly shown to decrease nitrite generation, taken as an index for NO, but so far it was not clear which constituent of the complex flavonoid mixture mediated this effect. The purpose of this study was to elucidate whether the in vivo generated Pycnogenol metabolite M1 (δ-(3,4-dihydroxyphenyl)-γ-valerolactone) displayed any activity in the context of induction of iNOS expression and excessive NO production. For the first time we show that M1 inhibited nitrite production (IC(50) 1.3 μg/ml, 95% CI 0.96-1.70) and iNOS expression (IC(50) 3.8 μg/ml, 95% CI 0.99-14.35) in a concentration-dependent fashion. This exemplifies bioactivation by metabolism because the M1 precursor molecule catechin is only weakly active. However, these effects required application of M1 in the low-micromolar range, which was not consistent with concentrations previously detected in human plasma samples after ingestion of maritime pine bark extract. Thus, we investigated a possible accumulation of M1 in cells and indeed observed high-capacity binding of this flavonoid metabolite to macrophages, monocytes, and endothelial cells. This binding was distinctly decreased in the presence of the influx inhibitor phloretin, suggesting the contribution of a facilitated M1 transport into cells. In fact, intracellular accumulation of M1 could explain why in vivo bioactivity can be observed with nanomolar plasma concentrations that typically fail to exhibit measurable activity in vitro. PMID:22569413

  9. Apples: content of phenolic compounds vs. variety, part of apple and cultivation model, extraction of phenolic compounds, biological properties.

    Science.gov (United States)

    Kalinowska, Monika; Bielawska, Aleksandra; Lewandowska-Siwkiewicz, Hanna; Priebe, Waldemar; Lewandowski, Włodzimierz

    2014-11-01

    Apples are among the most popular fruits in the world. They are rich in phenolic compounds, pectin, sugar, macro- and microelements. Applying different extraction techniques it is possible to isolate a particular group of compounds or individual chemicals and then test their biological properties. Many reports point to the antioxidant, antimicrobial, anticancer and many other beneficial effects of apple components that may have potential applications in food, pharmaceutical and cosmetic industries. This paper summarizes and compiles information about apple phenolic compounds, their biological properties with particular emphasis on health-related aspects. The data are reviewed with regard to different apple varieties, part of apple, cultivation model and methods of extraction. PMID:25282014

  10. Back-transmission of a virus associated with apple stem pitting and pear vein yellows from Nicotiana occidentalis to apple and pear indicators

    OpenAIRE

    Leone, G.; Lindner, J. L.; Jongedijk, G.; Meer, van der, D

    1995-01-01

    The successful back-transmission of the mechanically transmissible virus associated with apple stem pitting and pear vein yellows, from Nicotiana occidentalis to apple seedlings "Golden Delicious" under greenhouse conditions is reported. This result enabled a field experiment where isolates of apple stem pitting, originating from apple, that were back transmitted from N. occidentalis 37B and N. occidentalis subsp. obliqua to apple seedlings are indexed by double budding on apple and pear indi...

  11. A LC-MS metabolomics approach to investigate the effect of raw apple intake in the rat plasma metabolome

    DEFF Research Database (Denmark)

    Rago, Daniela; Kristensen, Mette; Gürdeniz, Gözde;

    2013-01-01

    the postprandial to the fasting state and affect steroid metabolism by suppressing the plasma level of stress corticosteroids, certain mineralocorticoids and oxidised bile acid metabolites. Several new hypotheses regarding the cause of health effects from apple intake can be generated from this study for further......Fruit and vegetable consumption has been associated with several health benefits; however the mechanisms are largely unknown at the biochemical level. Our research aims to investigate whether plasma metabolome profiling can reflect biological effects after feeding rats with raw apple by using...... dihydrochloride (DMH) once a week. Plasma samples were taken at the end of the intervention and among all groups, about half the animals were 12 h fasted. An initial ANOVA-simultaneous component analysis with a three-factor or two-factor design was employed in order to isolate potential metabolic variations...

  12. Phenotypic and Molecular Screening of Apple Genotypes to Woolly Apple Aphid Resistance

    Directory of Open Access Journals (Sweden)

    Saeid ABU-ROMMAN

    2014-06-01

    Full Text Available Woolly apple aphid (WAA is a major pest of apple orchards resulting in significant losses. In the present study, 22 apple (Malus domestica Borkh. cultivars were tested in the filed for their relative resistance/susceptibility to WAA. These apple cultivars were found to respond differentially to WAA infestation. Based on tree infestation rating, cultivars were ranked into 6 relative resistance/susceptibility groups, as follows: immune - ‘Golden Delicious’, ‘Delbarestivale’, ‘Golden Smoothie’, ‘Red Miracle’ and ‘Harmony’; resistant - ‘Stark Gold’, ‘Early Gold’ and ‘Argi Gala’; moderately resistant - ‘Evanee’ and ‘Black Stayman’; moderately susceptible - ‘Vista Bella’, ‘Jonagold’, ‘Royal Gala’, ‘Jersey Mac’, ‘Granny Spur Type’ and  ‘Summerred’; susceptible - ‘Jonathan’, ‘Nagava 6’, ‘Florina’, ‘Red Chief’ and ‘Gold Iralis’; highly susceptible - ‘Fuji 6’. Eight molecular markers linked to major WAA resistant genes (Er1, Er2, and Er3 were screened in apple cultivars using PCR. The markers NZms_EB145764, NZms_EB106753 and NZsc_E01 were ubiquitous in all cultivars under study, whereas, NZsn_O05 was absent. The results of other markers revealed distinct patterns of amplification among apple cultivars. No clear correlations can be made between the molecular data (marker presence and absence and the phenotypic results (cultivar ranking. The differences among cultivars regarding WAA infestation can potentially be utilized by apple breeders and commercial growers to achieve effective, environmental-friendly, and low-cost pest control.

  13. Production of Metabolites

    DEFF Research Database (Denmark)

    2011-01-01

    A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said...... micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid...

  14. [Antiviral properties of basidiomycetes metabolites].

    Science.gov (United States)

    Avtonomova, A V; Krasnopolskaya, L M

    2014-01-01

    The data on the antiviral action of the Ganoderma lucidum, Lentinus edodes, Grifola frondosa, Agaricus brasiliensis and other basidiomycetes metabolites are summurized. The metabolites of these species of basidiomycetes exhibit a direct antiviral effect on herpes simplex virus types I and II, human immunodeficiency virus (HIV), hepatitis B virus, vesicular stomatitis virus, influenza virus, Epstein-Barr virus, and others. Moreover, metabolites of basidiomycetes increased antiviral immunity. PMID:25975107

  15. Functional genomics reveals that a compact terpene synthase gene family can account for terpene volatile production in apple.

    Science.gov (United States)

    Nieuwenhuizen, Niels J; Green, Sol A; Chen, Xiuyin; Bailleul, Estelle J D; Matich, Adam J; Wang, Mindy Y; Atkinson, Ross G

    2013-02-01

    Terpenes are specialized plant metabolites that act as attractants to pollinators and as defensive compounds against pathogens and herbivores, but they also play an important role in determining the quality of horticultural food products. We show that the genome of cultivated apple (Malus domestica) contains 55 putative terpene synthase (TPS) genes, of which only 10 are predicted to be functional. This low number of predicted functional TPS genes compared with other plant species was supported by the identification of only eight potentially functional TPS enzymes in apple 'Royal Gala' expressed sequence tag databases, including the previously characterized apple (E,E)-α-farnesene synthase. In planta functional characterization of these TPS enzymes showed that they could account for the majority of terpene volatiles produced in cv Royal Gala, including the sesquiterpenes germacrene-D and (E)-β-caryophyllene, the monoterpenes linalool and α-pinene, and the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene. Relative expression analysis of the TPS genes indicated that floral and vegetative tissues were the primary sites of terpene production in cv Royal Gala. However, production of cv Royal Gala floral-specific terpenes and TPS genes was observed in the fruit of some heritage apple cultivars. Our results suggest that the apple TPS gene family has been shaped by a combination of ancestral and more recent genome-wide duplication events. The relatively small number of functional enzymes suggests that the remaining terpenes produced in floral and vegetative and fruit tissues are maintained under a positive selective pressure, while the small number of terpenes found in the fruit of modern cultivars may be related to commercial breeding strategies. PMID:23256150

  16. Functional Genomics Reveals That a Compact Terpene Synthase Gene Family Can Account for Terpene Volatile Production in Apple1[W

    Science.gov (United States)

    Nieuwenhuizen, Niels J.; Green, Sol A.; Chen, Xiuyin; Bailleul, Estelle J.D.; Matich, Adam J.; Wang, Mindy Y.; Atkinson, Ross G.

    2013-01-01

    Terpenes are specialized plant metabolites that act as attractants to pollinators and as defensive compounds against pathogens and herbivores, but they also play an important role in determining the quality of horticultural food products. We show that the genome of cultivated apple (Malus domestica) contains 55 putative terpene synthase (TPS) genes, of which only 10 are predicted to be functional. This low number of predicted functional TPS genes compared with other plant species was supported by the identification of only eight potentially functional TPS enzymes in apple ‘Royal Gala’ expressed sequence tag databases, including the previously characterized apple (E,E)-α-farnesene synthase. In planta functional characterization of these TPS enzymes showed that they could account for the majority of terpene volatiles produced in cv Royal Gala, including the sesquiterpenes germacrene-D and (E)-β-caryophyllene, the monoterpenes linalool and α-pinene, and the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene. Relative expression analysis of the TPS genes indicated that floral and vegetative tissues were the primary sites of terpene production in cv Royal Gala. However, production of cv Royal Gala floral-specific terpenes and TPS genes was observed in the fruit of some heritage apple cultivars. Our results suggest that the apple TPS gene family has been shaped by a combination of ancestral and more recent genome-wide duplication events. The relatively small number of functional enzymes suggests that the remaining terpenes produced in floral and vegetative and fruit tissues are maintained under a positive selective pressure, while the small number of terpenes found in the fruit of modern cultivars may be related to commercial breeding strategies. PMID:23256150

  17. Apple detection using infrared thermal image, 3: Real-time temperature measurement of apple tree

    International Nuclear Information System (INIS)

    In Part 1, we reported the thermal distribution characteristics and the identification methods of apples, leaves and branches by using the infrared thermal image at the specific time. This paper reports the temperature changing characteristics and the relationships among apples, leaves and air temperature based on the information measured by the infrared thermal image equipment in the real-time for 24 hours. As a result, it was confirmed that the average temperature of apples was 1 degree C or more higher than the one of the leaves, and the average temperature of the leaves was almost same as the air temperature within daytime and about 3 hours period after sunset. It was also clarified for a remarkable temperature difference not to exist for midnight and the early morning between the apples and the leaves, and both became almost as well as the air temperature. Moreover, a binary image was easily obtained and the apples could be detected by using this temperature difference informat

  18. Genetic Diversity of a Natural Population of Apple stem pitting virus Isolated from Apple in Korea.

    Science.gov (United States)

    Yoon, Ju Yeon; Joa, Jae Ho; Choi, Kyung San; Do, Ki Seck; Lim, Han Cheol; Chung, Bong Nam

    2014-06-01

    Apple stem pitting virus (ASPV), of the Foveavirus genus in the family Betaflexiviridae, is one of the most common viruses of apple and pear trees. To examine variability of the coat protein (CP) gene from ASPV, eight isolates originating from 251 apple trees, which were collected from 22 apple orchards located in intensive apple growing areas of the North Gyeongsang and North Jeolla Provinces in Korea, were sequenced and compared. The nucleotide sequence identity of the CP gene of eight ASPV isolates ranged from 77.0 to 97.0%, while the amino acid sequence identity ranged from 87.7 to 98.5%. The N-terminal region of the viral CP gene was highly variable, whereas the C-terminal region was conserved. Genetic algorithm recombination detection (GARD) and single breakpoint recombination (SBP) analyses identified base substitutions between eight ASPV isolates at positions 54 and 57 and position 771, respectively. GABranch analysis was used to determine whether the eight isolates evolved due to positive selection. All values in the GABranch analysis showed a ratio of substitution rates at non-synonymous and synonymous sites (dNS/dS) below 1, suggestive of strong negative selection forces during ASPV CP history. Although negative selection dominated CP evolution in the eight ASPV isolates, SLAC and FEL tests identified four possible positive selection sites at codons 10, 22, 102, and 158. This is the first study of the ASPV genome in Korea. PMID:25289003

  19. Genetic Diversity of a Natural Population of Apple stem pitting virus Isolated from Apple in Korea

    Directory of Open Access Journals (Sweden)

    Ju Yeon Yoon

    2014-06-01

    Full Text Available Apple stem pitting virus (ASPV, of the Foveavirus genus in the family Betaflexiviridae, is one of the most common viruses of apple and pear trees. To examine variability of the coat protein (CP gene from ASPV, eight isolates originating from 251 apple trees, which were collected from 22 apple orchards located in intensive apple growing areas of the North Gyeongsang and North Jeolla Provinces in Korea, were sequenced and compared. The nucleotide sequence identity of the CP gene of eight ASPV isolates ranged from 77.0 to 97.0%, while the amino acid sequence identity ranged from 87.7 to 98.5%. The N-terminal region of the viral CP gene was highly variable, whereas the C-terminal region was conserved. Genetic algorithm recombination detection (GARD and single breakpoint recombination (SBP analyses identified base substitutions between eight ASPV isolates at positions 54 and 57 and position 771, respectively. GABranch analysis was used to determine whether the eight isolates evolved due to positive selection. All values in the GABranch analysis showed a ratio of substitution rates at non-synonymous and synonymous sites (dNS/dS below 1, suggestive of strong negative selection forces during ASPV CP history. Although negative selection dominated CP evolution in the eight ASPV isolates, SLAC and FEL tests identified four possible positive selection sites at codons 10, 22, 102, and 158. This is the first study of the ASPV genome in Korea.

  20. Radiation mutagenesis in selection of apple trees

    International Nuclear Information System (INIS)

    After X-radiation of grafts of antonovka apple trees, three groups of morphological mutants, namely, weak-, average- and violently-growing, have been revealed. Although the mutation spectrum has some indefinite character a dose of 6 kR causes, more frequently and in a greater number, the weak-growing mutants, and a dose of 2 kR, the violently-growing ones. Mutants of each group differ in the precociousness (precocious and latefruiting), type of fruiting (nospur and spur) and yield (high- and low-yielding). Using the method of radiation mutagenesis it is possible to rise the frequency and spectrum of somatic mutability of antonovka apple trees and to induce forms having valuable features

  1. Camapple: CAMAC interface to the Apple computer

    International Nuclear Information System (INIS)

    The advent of the 'personal' microcomputer provides a new tool for the debugging, calibration and monitoring of small scale physics apparatus, e.g., a single detector being developed for a larger physics apparatus. With an appropriate interface these microcomputer systems provide a low cost (1/3 the cost of a comparable minicomputer system), convenient, dedicated, portable system which can be used in a fashion similar to that of portable oscilliscopes. Here we describe an interface between the Apple computer and CAMAC which is now being used to study the detector for a Cerenkov ring-imaging device. The Apple is particularly well-suited to this application because of its ease of use, hi-resolution graphics, peripheral bus and documentation support. (orig.)

  2. Apple iPhone拆解分析

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Apple iPhone是一款多功能高端手持设备,同时也具有移动媒体播放器、网络浏览器功能,其3.5英寸多触点触摸屏带16M色彩,160dpi时分辨率为480×320。其无线通信功能包括WiFi(802.11b/g)、EDGE、蓝牙2.0+EDR。该手机还带有2百万像素相机模块、SMS、网络浏览、照片编辑等功能,它采用Apple 0S X版本操作系统。

  3. Apple and Samsung Mobile Devices Comparison

    OpenAIRE

    Bureš, Jakub

    2013-01-01

    Bachelor thesis presents a comparison of mobile devices, Apple and Samsung, in particular the characteristics of the individual companies and the historical development of their mobile devices. It is focused on a comparison of selected mobile devices and finally discusses the major patent wars waged between the two companies. At the same time bachelor's thesis in his research points to the specific preferences of users of mobile devices.

  4. Analytical characterization of some pasteurized apple juices during storage

    Directory of Open Access Journals (Sweden)

    Damian Cristina

    2015-06-01

    Full Text Available The aim of the study was to assess the effect of three weeks of storage on the chemical and rheological properties of apple juices obtained from Idared and Jonatan apples variety. Total antioxidant activity, levels of bio-active compound groups and the viscosity were measured to characterize the investigated juices. The method applied for the determination of ascorbic acid concentration was with 2, 6-diclorophenolindophenol. Total phenols (TP in apple juices were determined using the Folin-Ciocalteau method and antioxidant activity by the use of DPPH free radical method. The viscosity of apple juices was investigated by a rotational viscometer, Brookfield viscometer (Brookfield Engineering Inc., Model RV-DV I Prime with RV spindles. During three weeks of storage, different rates of all measured properties have been observed decreasing for both studied apples varieties juices. The juices from Jonatan apples have higher antioxidant activities that are correlated with the higher content in polyphenols and lower values of viscosity.

  5. Multivariate analysis for selecting apple mutants

    International Nuclear Information System (INIS)

    The mutlivariate analysis of four year records on several vegetative and productive traits of twenty-one apple mutants (3 of 'Jonathan', 3 of 'Ozark Gold', 14 of 'Mollie's Delicious', 1 of 'Neipling's Early Stayman)' induced by gamma radiations showed that observation of some traits of one-year-old shoots is the most efficient way to reveal compact growing apple mutants. In particular, basal cross-section area, total length and leaf area resulted the most appropriate parameters, while internode length together with conopy height and width are less appropriate. The most interesting mutants we found are: one of 'Mollie's Delicious for the best balance among tree and fruit traits and for high skin color; one of 'Neipling's Early Stayman' with an earlier and more extensively red colored apple than the original clone. (author)

  6. Sustainability of three apple production systems.

    Science.gov (United States)

    Reganold, J P; Glover, J D; Andrews, P K; Hinman, H R

    2001-04-19

    Escalating production costs, heavy reliance on non-renewable resources, reduced biodiversity, water contamination, chemical residues in food, soil degradation and health risks to farm workers handling pesticides all bring into question the sustainability of conventional farming systems. It has been claimed, however, that organic farming systems are less efficient, pose greater health risks and produce half the yields of conventional farming systems. Nevertheless, organic farming became one of the fastest growing segments of US and European agriculture during the 1990s. Integrated farming, using a combination of organic and conventional techniques, has been successfully adopted on a wide scale in Europe. Here we report the sustainability of organic, conventional and integrated apple production systems in Washington State from 1994 to 1999. All three systems gave similar apple yields. The organic and integrated systems had higher soil quality and potentially lower negative environmental impact than the conventional system. When compared with the conventional and integrated systems, the organic system produced sweeter and less tart apples, higher profitability and greater energy efficiency. Our data indicate that the organic system ranked first in environmental and economic sustainability, the integrated system second and the conventional system last. PMID:11309616

  7. Sensory quality of scab-resistant apple cultivars

    OpenAIRE

    Kühn, Senior Scientist Birka Falk; Thybo, Senior Scientist Anette

    2001-01-01

    Twenty-two scab-resistant apple cultivars were harvested in autumn 1999 and evaluated for sensory quality the following October, November and December. Multivariate analysis was effective in describing the comblex relationships and variabillity among the numerous attributes used to characterise apple quality. Crispness, mealiness, skin toughness, apple flavour, sweetness, unripe flavour and overripe flavour were informative attributes describing the variation in the sensory quality. Texture a...

  8. Porovnání platformy Apple a PC

    OpenAIRE

    Bazhan, Alexander

    2014-01-01

    This bachelor work, Apple and PC Platform Comporasion is devided into four sections. First part shows evolution of Apple and gives a representation of development following operating systems: Mac OS and Windows. Second part contains definition of the main differences between Mac and PC platforms. Third part tells about comparaison of the most modern operation systems from Apple and Microsoft. In fourth part operation systems will be compared with respect to speed and performance. Comparais...

  9. Industry Analysis: Apple Processors in the Northeastern U.S.

    OpenAIRE

    Rowles, Kristin L.

    2001-01-01

    Apple processors are an important link in the marketing chain from apple growers to consumers, and their perspective is critical in understanding the industry?s situation and projecting the industry?s future. This paper reports the results of a survey of Northeastern U.S. apple processors. The survey was conducted to provide a snapshot of current strategic issues in the industry, to assess the industry?s strengths and weaknesses, to identify opportunities and threats, to forecast future trend...

  10. Theory of Regression Apple Professional Cooperation Organization Research

    OpenAIRE

    Ouyang Bin

    2013-01-01

    In view of the enterprise ecological apple manor a variety of problems of existence, put forward to the enterprise management transformation, achieve enterprise, collective, individual integrated operation management and the use of regression mathematical model on apple professional cooperation organization analysis. Through the example, Apple professional economic cooperation organization innovation model of the input output ratio than the rural economic cooperation organization is much high...

  11. The Venturia Apple Pathosystem: Pathogenicity Mechanisms and Plant Defense Responses

    Directory of Open Access Journals (Sweden)

    Gopaljee Jha

    2009-01-01

    Full Text Available Venturia inaequalis is the causal agent of apple scab, a devastating disease of apple. We outline several unique features of this pathogen which are useful for molecular genetics studies intended to understand plant-pathogen interactions. The pathogenicity mechanisms of the pathogen and overview of apple defense responses, monogenic and polygenic resistance, and their utilization in scab resistance breeding programs are also reviewed.

  12. Analytical characterization of some pasteurized apple juices during storage

    OpenAIRE

    Damian Cristina; Leahu Ana; Oroian Mircea; Ropciuc Sorina

    2015-01-01

    The aim of the study was to assess the effect of three weeks of storage on the chemical and rheological properties of apple juices obtained from Idared and Jonatan apples variety. Total antioxidant activity, levels of bio-active compound groups and the viscosity were measured to characterize the investigated juices. The method applied for the determination of ascorbic acid concentration was with 2, 6-diclorophenolindophenol. Total phenols (TP) in apple juices were determined using the Folin-C...

  13. Quantification Model for Estimating Temperature Field Distributions of Apple Fruit

    OpenAIRE

    Min ZHANG; Yang, Le; Zhao, Huizhong; Zhang, Leijie; Zhong, Zhiyou; Liu, Yanling; Chen, Jianhua

    2010-01-01

    A quantification model of transient heat conduction was provided to simulate apple fruit temperature distribution in the cooling process. The model was based on the energy variation of apple fruit of different points. It took into account, heat exchange of representative elemental volume, metabolism heat and external heat. The following conclusions could be obtained: first, the quantification model can satisfactorily describe the tendency of apple fruit temperature distribution in the cooling...

  14. New Products against Apple Scab and Powdery Mildew Attack in Organic Apple Production

    Directory of Open Access Journals (Sweden)

    Viorel MITRE

    2010-12-01

    Full Text Available Interspecific hybridizations represent one of the apple breeding methods by which a wide variability can be achieved, useful for creating new cultivars. The study of 2190 interspecific hybrids, obtained from 25 combinations among crab apple species (Malus coronaria, M. floribunda, M. niedzwetzkyana, M. zumi, M. prunifolia and different apple cultivars, points out a large variability of the F1 seedlings for several traits, with significant importance in apple breeding programs. The first year of fructification, as mean per hybrid combination, varied from 6 (M. zumi x ‘Jonathan’ to 9.3 years; the average hybrid’s age for fructification was 7.4 years. The size of fruits among families varied from 1.5 (‘Starkrimson’ x M. prunifolia to 4.0 (‘Reinette Baumann’ x M. zumi, the mean being settled at 2.8 (therefore below mark 3, meaning ‘small fruits’. The lowest infection rate both for apple scab and powdery mildew attack was noticed at hybrids from M. coronaria x ‘Reinette Baumann’. On the whole, the hybrids with genitors of ‘species x cultivars’ type have had an early fruiting and a better resistance to scab and powdery mildew, compared to the ones from ‘cultivars x species’ combinations. The large variability of the studied traits gave the possibility to identify offsprings with desirable characteristics on nine hybrid combinations. Among these, 53 elite plants were selected, with a strength of selection of 2.42%, therefore a relative low value, correlated with the peculiarities of the interspecific population and the selection criteria for dessert apple.

  15. Extraction of pectin from apple pomace

    Directory of Open Access Journals (Sweden)

    Maria Helene Canteri-Schemin

    2005-03-01

    Full Text Available As apple-processing units are now in expansion in Brazil, industrial by-products like pomace play an important role in pectin manufacture. The objective of this article was to determine a pratical follow-up to the extraction of pectin from apple pomace and to characterize it in a laboratory, on a small scale, aiming at establishing the optimum conditions for acid extraction. The highest yields were obtained when [1]apple pomace was dried and ground to obtain an apple flour to be used as raw material, [2] citric or nitric acids were used and [3] when the citric acid concentration was 6.2 g/100 ml and the time of reaction was 153 minutes. The apple variety in itself was not significant in pectin yield. The degree of esterification (DE = 68.84 % of the product obtained, as well as its physical looks, show the success of pectin extraction.No aproveitamento de resíduos das indústrias processadoras de maçã, em expansão no Brasil, a pectina alimentícia surge como importante subproduto. Os objetivos deste trabalho foram os seguintes: extração e caracterização da pectina de bagaço de maçã em bancada, em que as condições para maior rendimento de obtenção em relação ao tipo de ácido, ao tamanho das partículas e à variedade de maçã foram estabelecidas. Objetivou-se também a otimização da produção, levando-se em conta o tempo e a concentração do ácido cítrico. Foram considerados métodos de extração mais eficientes quanto ao rendimento: [1] acidificação com ácido cítrico ou ácido nítrico, [2] cominuição do bagaço, usando a farinha como matéria-prima, com partículas retidas no tamis de 106 µm, [3] concentração de ácido de 6,2 g% e tempo de extração de 153 minutos. A variedade da maçã não interferiu significativamente no rendimento. A alta metoxilação (DE= 68,84% das pectinas produzidas em bancada confirma o grau de maturidade das matérias-primas usadas nos experimentos.

  16. Studies on apple preservation by 60 -gamma irradiation

    International Nuclear Information System (INIS)

    Studies on '60'Co-gamma irradiation of Golden Delicious apple have been carried out. The results showed that the optimum irradiation dosage for stored apple was ranged from 0.3-0.5 k Gy, with this dosage, the contents of vitamin c and titratalbe acidity in these apples had no significant change compared with unirradiated apples. The respiratory rate and the amount of ethylene release were decreased after irradiation. The mortality of the verticillate pathogenic fungi was 97% at the dose of 0.5 k Gy

  17. Theory of Regression Apple Professional Cooperation Organization Research

    Directory of Open Access Journals (Sweden)

    Ouyang Bin

    2013-03-01

    Full Text Available In view of the enterprise ecological apple manor a variety of problems of existence, put forward to the enterprise management transformation, achieve enterprise, collective, individual integrated operation management and the use of regression mathematical model on apple professional cooperation organization analysis. Through the example, Apple professional economic cooperation organization innovation model of the input output ratio than the rural economic cooperation organization is much higher, the conclusion and the utility of the marginal analysis theory is contrary to. Conclusion the apple professional economic cooperation organization of research and development has a certain reference value.

  18. The time of infection of apples by Botrytis cinerea Pers.

    OpenAIRE

    Hanna Bryk

    2013-01-01

    The time of infection of apple fruits by Botrytis cinerea Pers. was studied. Artificial inoculations with conidial suspensions of B. cinerea were done at different stages of fruit developmment (flowers, sets, fruits). In autumn the apples were harvested and stored at a temperature of 2°C for 4 months after which rotting caused by B. cinerea was evaluated. B. cinerea presence in the calyx of apples was checked throughout the growing season. This was done by plating flowers, apple and set calyc...

  19. Sun, shade, and secondary metabolites

    Science.gov (United States)

    My research program focuses on understanding plant primary and secondary metabolites. Grape secondary metabolites, such as phenolics, have long been valuable for the organoleptic properties they impart to fruit and wine, and, more recently, for their possible health benefits. These compounds develop...

  20. Secondary metabolites from Eremostachys laciniata

    DEFF Research Database (Denmark)

    Calis, Ihsan; Güvenc, Aysegül; Armagan, Metin;

    2008-01-01

    metabolites were elucidated from spectroscopic (UV, IR, 1D- and 2D-NMR) and ESI-MS evidence, as well as from their specific optical rotation. The presence of these metabolites of three different classes strongly supports the close relationship of the genera Eremostachys and Phlomis....

  1. STUDY ON THE MACRONUTRIENT CONTENT OF APPLE LEAVES IN AN ORGANIC APPLE ORCHARD

    OpenAIRE

    Nagy, Péter; Imre HOLB

    2006-01-01

    Macronutrient contents of soil and apple leaves were investigated in an organic apple orchard in Eastern Hungary in 2002-2004. Soil samples were taken from 0-20 cm depth in April and October. The macronutrient content of leaves was measured on cvs. Jonagold, Mutsu, Idared, Red Elstar, Egri Piros, Reka, and Remo at six assessment dates (from April to September). The macronutrient contents of N, P, K, S, Ca and Mg were measured in both soil and leaf samples, then macronutrient ratios were ca...

  2. Developing Data Base Files Using the AppleWorks Data Base Subprogram and Apple IIe or GS Computers.

    Science.gov (United States)

    Schlenker, Richard M.

    Developed around the technology of the Apple duodisk drive, five and one-fourth inch floppy disks, and the 1.3 version of the AppleWorks program, this manual is designed for use as a "how to" training device in developing database files. The guide is meant to be used with Apple IIe or IIGS computers which have a duodisk or two disk drives and an…

  3. Assessing sustainability of organic apple orchards. The case of small scale apple production in Ningxia Province, PR China

    OpenAIRE

    Wong, Louisa

    2014-01-01

    China produces 43% of the world apple supply, but low fruit quality remains a problem for export of table fruit. Ningxia province is also facing challenges such as low soil fertility, poor orchard infrastructure and inadequate institutional support for small-scale apple producers. The study was carried out to assess a. the sustainability of organic apple orchards and b. the potential role of Organic and Fair Trade certification to contribute to farm sustainability and improve socio-economic o...

  4. Formulation of Apple Sauce Suitable for the Taste of Iranian Consumers

    OpenAIRE

    J. Jamalian; GH. Mesbahi

    2006-01-01

    Iran is a major apple producing country. A number of industrial apple products can be made from apple. In some developed countries about 25% of apples are processed into apple sauce. Apple sauce hasn’t so far been produced by Iranian food industry, but if a formulation suitable for the taste of Iranian consumers is prepared, it can commercially succeed as a consumer product. At the first step of this study, six apple sauce formulations were made from Golden Delicious apples. Then the sensory ...

  5. The Effect of Apple Feeding on Markers of Colon Carcinogenesis

    DEFF Research Database (Denmark)

    Poulsen, Morten; Mortensen, Alicja; Binderup, Mona-Lise;

    2011-01-01

    not 10 g had a significantly lower number of ACF (P = 0.009) compared to the initiated control. DNA damage evaluated by the comet assay was significantly increased in leucocytes of DMH-treated animals (P = 0.021). No antigenotoxic effect of apple feeding was apparent in the colon. Apple feeding tended...

  6. Nutritional enrichment of fresh apple (Royal Gala) by vacuum impregnation.

    Science.gov (United States)

    Xie, J; Zhao, Y

    2003-09-01

    This study evaluates the use of vacuum impregnation (VI) for developing nutritionally fortified fresh cut apples (Royal Gala). Cut apples were immersed in diluted high fructose corn syrup (20% w/w or 50% w/w) containing calcium or zinc. A vacuum pressure of 50 mmHg was applied for 15 min following atmospheric pressure restoration for 30 min while samples remained in the VI solution. Nutraceutical content and physicochemical properties of the apples immediately after VI were determined. Storability of VI apples at 6 degrees C and 90% relative humidity was also studied based on the color and texture of apples. Results indicated that 15-20% of the Daily Reference Intake of calcium and above 40% of the Daily Reference Intake of zinc could be obtained in 200 g fresh cut apples. VI treatments in 20% w/w high fructose corn syrup solutions had little effects on the physicochemical properties of apples. Storage study showed that VI with zinc significantly improved color stability, and calcium enhanced the firmness of the apples. PMID:12907403

  7. Sharing the Arts of the Blue Ridge Mountains. Apple Dolls.

    Science.gov (United States)

    Holman, Martha; Gailey, Lamar

    This module on apple dolls is one of eight modules designed to provide instruction on authentic Blue Ridge Mountain crafts to adult basic education students at a low cost. Contents include notes on the apple doll making; process used, including equipment and materials, as well as method described narratively and graphically; and the followup,…

  8. Apple IIe Computers and Appleworks Training Mini Course Materials.

    Science.gov (United States)

    Schlenker, Richard M.

    The instructional materials included in this document are designed to introduce students to the Apple IIe computer and to the word processing and database portions of the AppleWorks program. The materials are intended for small groups of students, each of whom has use of a computer during class and for short periods between classes. The course…

  9. Formatting Data Disks for Use with the Apple IIe Computer.

    Science.gov (United States)

    Schlenker, Richard M.

    This step-by-step guide to formatting disks using the AppleWorks software on the Apple IIe computer covers (1) loading the program; (2) formatting the disk; (3) volume naming; and (4) exiting the format options. Eleven sample screen displays illustrate the steps. (MES)

  10. Chapter 11. Quality evaluation of apple by computer vision

    Science.gov (United States)

    Apple is one of the most consumed fruits in the world, and there is a critical need for enhanced computer vision technology for quality assessment of apples. This chapter gives a comprehensive review on recent advances in various computer vision techniques for detecting surface and internal defects ...

  11. Reading Michael Apple--The Sociological Imagination at Work

    Science.gov (United States)

    Ball, Stephen J.

    2007-01-01

    This article discusses Michael Apple's contribution to the sociology of education and education policy analysis and the politics of education. It focuses on ways of "reading" Apple as an intellectual and an activist and looks at the trajectory of his work over a long and illustrious career.

  12. Transcriptional response in apple to fire blight disease

    Science.gov (United States)

    Fire blight, caused by the bacterium Erwinia amylovora, is a destructive disease of apple, pear, and other plants in the subfamily Maloideae of the Rosaceae. The goal of this study was to use a global analysis of gene expression to characterize the temporal response of apple to infection by E. amyl...

  13. RT-PCR-ELISA for detection of Apple stem grooving virus in apple trees

    Directory of Open Access Journals (Sweden)

    Marinho Vera Lúcia A.

    2003-01-01

    Full Text Available A method to detect Apple stem grooving virus (ASGV based on reverse transcription polymerase chain reaction (RT-PCR was developed using primers ASGV4F-ASGV4R targeting the viral replicase gene, followed by a sandwich hybridisation, in microtiter plates, for colorimetric detection of the PCR products. The RT-PCR was performed with the Titan™ RT-PCR system, using AMV and diluted crude extracts of apple (Malus domestica leaf or bark for the first strand synthesis and a mixture of Taq and PWO DNA polymerase for the PCR step. The RT-PCR products is hybridised with both a biotin-labelled capture probe linked to a streptavidin-coated microtiter plate and a digoxigenin (DIG-labelled detection probe. The complex was detected with an anti-DIG conjugate labelled with alkaline phosphatase. When purified ASGV was added to extracts of plant tissue, as little as 400 fg of the virus was detected with this method. The assay with ASGV4F-ASGV4R primers specifically detected the virus in ASGV-infected apple trees from different origins, whereas no signal was observed with amplification products obtained with primers targeting the coat protein region of the ASGV genome or with primers specific for Apple chlorotic leaf spot virus (ACLSV and Apple stem pitting virus (ASPV. The technique combines the power of PCR to increase the number of copies of the targeted gene, the specificity of DNA hybridization, and the ease of colorimetric detection and sample handling in microplates.

  14. Marketing Google, Microsoft a Apple

    OpenAIRE

    Hlavatá, Kristina

    2009-01-01

    The goal is to bring a comprehensive overview of the situation of these companies in the IT sector. The work will be carried out general analysis of the evolution of marketing companies Google, Apple and Mircosoft. At the same time there will be a preview provided by the companies in economic development, which will be analyzed by means of electronic information resources. In the chapter entitled as A survey of this work are presented results of a survey in the chosen target group, which asse...

  15. Cell Biology Apps for Apple Devices

    OpenAIRE

    Stark, Louisa A.

    2012-01-01

    Apps for touch-pad devices hold promise for guiding and supporting learning. Students may use them in the classroom or on their own for didactic instruction, just-in-time learning, or review. Since Apple touch-pad devices (i.e., iPad and iPhone) have a substantial share of the touch-pad device market (Campbell, 2012), this Feature will explore cell biology apps available from the App Store. My review includes iPad and iPhone apps available in June 2012, but does not include courses, lectures,...

  16. Apple Trees - A Source of Energy

    International Nuclear Information System (INIS)

    There is a large quantity of leftover (cut-off) branches after the trimming of fruit trees, both during in winter or during the vegetation period. In intensive production, trimming most often occurs as a combined cut, which means it is done both manually and with the help of machines. The leftover mass has to be taken care of in both cases. This paper deals with the apple tree biomass in the last 10 years expressed in tons, covering both the winter and vegetation period, and also about the possibility of using this biomass to replace fossil fuels. (author)

  17. Instant Apple Configurator how-to

    CERN Document Server

    Edge, Charles

    2013-01-01

    Filled with practical, step-by-step instructions and clear explanations for the most important and useful tasks. Get the job done and learn as you go. A how-To book with practical recipes accompanied with rich screenshots for easy comprehension.This book follows the Packt How-to approach, offering an informative yet practical guide that is easy to understand. The book takes a meticulous approach to providing quick and simple recipe-based solutions for security and deployment of iOS devices.""Instant Apple iOS Configurator Utility How-to"" book is ideal for anyone with a working knowledge in iO

  18. Evaluation of the 14C-prochloraz residue levels in irradiated mangoes (Mangifera indica) and apples (Malus domestica)

    International Nuclear Information System (INIS)

    The Brazilian crops mangoes and apples has expanding in the last years. However, tropical fruits crops are susceptible to infection that harms the crop yield. The control of these diseases is made through fungicides such as prochloraz which possesses protecting eradicating action by controlling anthracnose in mangoes and scabies in apples. Agrochemicals are of great importance in the agriculture considering the of relationship cost/benefit. However they may cause a series of problems in the ecosystem, being the levels of agrochemicals residues in fruits one of these factors. The aim of this work was to evaluate the prochloraz levels in mangoes treated in both stages of pre and post harvest, and in apples treated in the pre harvest. In the treatment of the mangoes, the interaction, fungicide with the gamma radiation with dose of 1,0 kGy, was used to induce prochloraz degradation. Treated post harvest mangoes were stored, at 12 deg C during 21 days. The results showed that the prochloraz did not present reduction in the residual levels of the mangoes after 21 day storage, that is the safe period established by the Brazilian legislation on agrochemicals, in treated mangoes. The refrigerated storage (12 deg C) and the gamma radiation also did not contribute to the degradation of the fungicide in mangoes. In average the concentration of the fungicide prochloraz in the peels (bar X = 1,64 mug/g) was higher than in the mangoes pulp (bar x = 0,06 mug/g), which allows the consumption of this fruit, since the peel is always discarded. The degradation product, formed in peels of mangoes in fruits treated in the pre and post harvest was the metabolite BIS 44596; for mangoes treated in the pre-post harvest the metabolite formed was the BIS 445186. Both metabolites were found in very low levels, confirming that occurs degradation of the fungicide in mangoes. In apples treated with the prochloraz, reduction of 500/0 in the levels. (author)

  19. Using AppleWorks V1.3 To Construct Data Base Files for the Apple IIe Computer. Second Edition.

    Science.gov (United States)

    Schlenker, Richard M.

    This step-by-step guide to using AppleWorks V1.3 to construct database files for the Apple IIe computer covers (1) program loading; (2) selecting the add files to the desktop option; (3) naming the file; (4) the blinking block cursor; (5) naming categories or fields; (6) moving to insert data; (7) inserting data; (8) changing database file…

  20. Using AppleWorks V1.3 To Construct Word Processing Files for the Apple IIe Computer. Second Edition.

    Science.gov (United States)

    Schlenker, Richard M.

    This step-by-step guide to using AppleWorks V1.3 to construct word processing files for the Apple IIe computer covers (1) program loading; (2) selecting the add files to the desktop option; (3) selecting a different disk; (4) selecting disk 2; (5) selecting the word processor option; (6) naming the file; (7) setting tabs; (8) selecting print…

  1. Identification of irradiated apples for phytosanitary purposes

    Science.gov (United States)

    Horak, Celina I.; Di Giorgio, Marina; Kairiyama, Eulogia

    2009-07-01

    The irradiation treatment of fresh fruits and vegetables for phytosanitary purposes is a satisfactory alternative method to others like fumigation and cold and hot treatments. Its use is increasing in several countries, and at present its approval is under revision by the National Regulatory Authorities. To verify the control process, apart from irradiation and dosimetry certificates, National Authorities require complementary evidence to show the efficacy of this treatment, especially when the documentation is not clear. The irradiation of fresh fruits produces single and double fragmentation in the DNA molecule, which can be measured using the microgel electrophoresis of individual cell (comet assay). The purpose of this work was to evaluate if it is possible to identify the irradiated apples for phytosanitary purposes from the others that were not treated. The possibility to estimate the absorbed dose was also evaluated. The methodology was carried out on the cell suspension obtained from irradiated seed cells with incremental doses (100, 200 and 300 Gy). The irradiation treatment for phytosanitary purposes to avoid emergency of codling moth ( Cydia pomonella) is 200 Gy. The fragmentation produced in the irradiated samples was proportional with the incremental doses applied. These results show that with this methodology it can be determined if the apple was irradiated or not. This comet assay is a simple, economical and interesting method that can be used, in case of necessity, by the National Authorities.

  2. Identification of irradiated apples for phytosanitary purposes

    International Nuclear Information System (INIS)

    The irradiation treatment of fresh fruits and vegetables for phytosanitary purposes is a satisfactory alternative method to others like fumigation and cold and hot treatments. Its use is increasing in several countries, and at present its approval is under revision by the National Regulatory Authorities. To verify the control process, apart from irradiation and dosimetry certificates, National Authorities require complementary evidence to show the efficacy of this treatment, especially when the documentation is not clear. The irradiation of fresh fruits produces single and double fragmentation in the DNA molecule, which can be measured using the microgel electrophoresis of individual cell (comet assay). The purpose of this work was to evaluate if it is possible to identify the irradiated apples for phytosanitary purposes from the others that were not treated. The possibility to estimate the absorbed dose was also evaluated. The methodology was carried out on the cell suspension obtained from irradiated seed cells with incremental doses (100, 200 and 300 Gy). The irradiation treatment for phytosanitary purposes to avoid emergency of codling moth (Cydia pomonella) is 200 Gy. The fragmentation produced in the irradiated samples was proportional with the incremental doses applied. These results show that with this methodology it can be determined if the apple was irradiated or not. This comet assay is a simple, economical and interesting method that can be used, in case of necessity, by the National Authorities.

  3. Secondary metabolites from marine microorganisms

    Directory of Open Access Journals (Sweden)

    KELECOM ALPHONSE

    2002-01-01

    Full Text Available After 40 years of intensive research, chemistry of marine natural products has become a mature field. Since 1995, there are signals of decreased interest in the search of new metabolites from traditional sources such as macroalgae and octocorals, and the number of annual reports on marine sponges stabilized. On the contrary, metabolites from microorganisms is a rapidly growing field, due, at least in part, to the suspicion that a number of metabolites obtained from algae and invertebrates may be produced by associated microorganisms. Studies are concerned with bacteria and fungi, isolated from seawater, sediments, algae, fish and mainly from marine invertebrates such as sponges, mollusks, tunicates, coelenterates and crustaceans. Although it is still to early to define tendencies, it may be stated that the metabolites from microorganisms are in most cases quite different from those produced by the invertebrate hosts. Nitrogenated metabolites predominate over acetate derivatives, and terpenes are uncommon. Among the latter, sesquiterpenes, diterpenes and carotenes have been isolated; among nitrogenated metabolites, amides, cyclic peptides and indole alkaloids predominate.

  4. Creating, Storing, and Dumping Low and High Resolution Graphics on the Apple IIe Microcomputer System.

    Science.gov (United States)

    Fletcher, Richard K., Jr.

    This description of procedures for dumping high and low resolution graphics using the Apple IIe microcomputer system focuses on two special hardware configurations that are commonly used in schools--the Apple Dot Matrix Printer with the Apple Parallel Interface Card, and the Imagewriter Printer with the Apple Super Serial Interface Card. Special…

  5. 75 FR 11071 - Removal of Varietal Restrictions on Apples from Japan

    Science.gov (United States)

    2010-03-10

    ... Varietal Restrictions on Apples from Japan AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION... variety apples from Japan to allow all varieties of Malus domestica apples into the United States under... of M. domestica apples from Japan to be imported into the United States while continuing to...

  6. The history of apple breeding in People’s Republic Of China

    Science.gov (United States)

    China is the leading apple production country in the world. Its apple area and production in 2006 was 1,898,000 hectares and 26,000,000 tons, accounting for more than 35% of the global apple area and production, respectively. New cultivars are very important to development of the apple industry. Chi...

  7. Antioxidant and antiproliferative activity of Granny Smith apple pomace

    Directory of Open Access Journals (Sweden)

    Savatović Slađana M.

    2008-01-01

    Full Text Available Granny Smith apple pomace was subjected to evaluation as valuable source of antioxidant and anticancer phytochemicals on the basis of its content in phenolic compounds, antioxidant and antiproliferative activity. The total cotent of phenolics, flavonoids and flavan-3-ols in apple pomace determined spectrophotometrically, was 7.02 mg/g, 0.51 mg/g and 8.80 mg/g. Major phenolics (phenolic acids, flavan-3-ols, flavonoids and dihydrochalcons in apple pomace were identified and quantified by HPLC. The antioxidant activity of apple pomace on stable 1,1-diphenyl-2-picrylhydrazyl (DPPH and reactive hydroxyl radicals, was investigated by electron spin resonance (ESR spectroscopy. The IC50 DPPH and IC50 OH values of Granny Smith apple pomace were 9.51 mg/ml and 29.17 mg/ml, respectively. The antiproliferative activities of apple pomace on cervix epitheloid carcinoma (HeLa, colon adenocarcinoma (HT-29 and breast adenocarcinoma (MCF7 cell lines were determined according to the MTT (3-(4,5-dimethylthiazol-2-yl- 2,5-diphenyltetrazolium bromide colorimetric assay. The IC50 HeLa , IC50 HT-29 and IC50 MCF7 values of Granny Smith apple pomace were 26.40 mg/ml, 22.47 mg/ml and 21.26 mg/ml, respectively. The significant correlations between antioxidant activities and antiproliferative activities were established (p<0.05.

  8. Carbendazim and metalaxyl residues in post-harvest treated apples.

    Science.gov (United States)

    Su, Yirong; Mitchell, S H; Mac AntSaoir, S

    2003-08-01

    Bramley apples were treated with Ridomil mbc 60 WP, containing carbendazim and metalaxyl, at half and full rate application at 10 and 20 degrees C, and with diphenylamine to control disease during storage. Carbendazim and metalaxyl residues were determined by HPLC and GC/MS, respectively, in apple core, flesh and peel at the initial time, 3 and 6 months after storage under controlled atmosphere conditions of 4.5 degrees C, 5% CO(2) and 1% O(2). The concentration of carbendazim residues in apple flesh was metalaxyl residues in apple flesh was metalaxyl in apple was 1.0 mg kg(-1). The temperature of the fungicide solution had little effect on the carbendazim residues but did have some effect on metalaxyl residues. Carbendazim residue content per apple was decreased when diphenylamine was included. A change in the ratio of carbendazim to metalaxyl was noted in the flesh, core and peel of the stored apple. The ratio of carbendazim to metalaxyl was 5:1 in the fungicide mix as applied. The ratio remained fairly constant in the core during storage. However, the ratio changed significantly in the peel and, to a lesser extent, and in a different direction, in the flesh. In the peel, the ratio was around 15:1 at 3 months of storage and 12:1 after 6 months of storage, whereas for flesh the ratios were 2:1 and 4:1, respectively. PMID:13129789

  9. Development of compact mutants in apple and sour cherry

    International Nuclear Information System (INIS)

    During the period 1973 - 79 studies were conducted with the aim of developing compact mutants in apple and cherry cultivars and in apple vegetative rootstocks. During the investigations the effect of the dose of gamma rays on frequency of the mutants was studied. Attempts were also made to evolve a micropropagation technique adapted to propagate P 2 and P 22 apple rootstocks, as an aid in mutation breeding. Several mutants were produced in all the material studied, but none of them have yet reached a sufficient developmental stage to enable their complete assessment. On the basis of the results obtained so far the following conclusions can be drawn: higher doses of irradiation resulted in higher frequency of mutants in most apple cultivars and apple rootstocks; in sour cherries the effect of dose depended on the cultivars. Among V1 shoots developed from sleeping buds on irradiated scion wood, compact mutants were found; their frequency, however, was about 60% lower than among V1 shoots developed directly from irradiated dormant buds. In apple rootstocks A 2 and M 26 several dwarfed mutants were found; some of these produced thorny plants and some had lower rooting ability; both these characteristics are inferior from the practical point of view. Multiplication and rooting media for in vitro propagation of apple rootstocks, worked out for M 26, were found unsuitable for the rootstocks P 2 and P 22; modifications made in the growth substance composition of the above media enabled satisfactory propagation to be obtained. (author)

  10. Yin-Yang Regulation of Adiponectin Signaling by APPL Isoforms in Muscle Cells*

    OpenAIRE

    Wang, Changhua; Xin, Xiaoban; Xiang, Ruihua; Ramos, Fresnida J.; Liu, Meilian; Lee, Hak Joo; Chen, Hongzhi; Mao, Xuming; Kikani, Chintan K.; Liu, Feng; Dong, Lily Q.

    2009-01-01

    APPL1 is a newly identified adiponectin receptor-binding protein that positively mediates adiponectin signaling in cells. Here we report that APPL2, an isoform of APPL1 that forms a dimer with APPL1, can interacts with both AdipoR1 and AdipoR2 and acts as a negative regulator of adiponectin signaling in muscle cells. Overexpression of APPL2 inhibits the interaction between APPL1 and AdipoR1, leading to down-regulation of adiponectin signaling in C2C12 myotubes. In contrast, suppressing APPL2 ...

  11. Production System of Virus-free Apple Plants Using Heat Treatment and Shoot Tip CultureShoot Tip Culture

    OpenAIRE

    Gunsup Lee; Il Sheob Shin; Kang Hee Cho; Se Hee Kim; Juhee Shin; Dae Hyun Kim; Jeong Hee Kim; Hyun Ran Kim

    2013-01-01

    In worldwide, viral diseases of apple plants has caused the serious problems like reduced production and malformation of fruits. Also, the damages of apple plants by virus and/or viroid infection (Apple chlorotic leaf spot virus, Apple stem grooving virus, Apple mosaic virus, and Apple scar skin viroid) were reported in Korea. However there is few report about the protection approach against the infection by apple viruses. Therefore, this paper introduced the experimental protocol...

  12. Apple iPhone%Apple iPhone拆解分析

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Apple iPhone是一款多功能高端手持设备,同时也具有移动媒体播放器、网络浏览器功能,其3.5英寸多触点触摸屏带16M色彩,160dpi时分辨率为480×320。其无线通信功能包括WiFi(802.11b/g)、EDGE、蓝牙2.0+EDR。该手机还带有2百万像素相机模块、SMS、网络浏览、照片编辑等功能,它采用Apple 0S X版本操作系统。

  13. The Apple of the mind's eye: Everyday attention, metamemory, and reconstructive memory for the Apple logo.

    Science.gov (United States)

    Blake, Adam B; Nazarian, Meenely; Castel, Alan D

    2015-01-01

    People are regularly bombarded with logos in an attempt to improve brand recognition, and logos are often designed with the central purpose of memorability. The ubiquitous Apple logo is a simple design and is often referred to as one of the most recognizable logos in the world. The present study examined recall and recognition for this simple and pervasive logo and to what degree metamemory (confidence judgements) match memory performance. Participants showed surprisingly poor memory for the details of the logo as measured through recall (drawings) and forced-choice recognition. Only 1 participant out of 85 correctly recalled the Apple logo, and fewer than half of all participants correctly identified the logo. Importantly, participants indicated higher levels of confidence for both recall and recognition, and this overconfidence was reduced if participants made the judgements after, rather than before, drawing the logo. The general findings did not differ between Apple and PC users. The results provide novel support for theories of attentional saturation, inattentional amnesia, and reconstructive memory; additionally they show how an availability heuristic can lead to overconfidence in memory for logos. PMID:25721103

  14. Tolerance to water deficit in young trees of jackfruit and sugar apple Tolerância ao déficit hídrico em plantas jovens de jaqueira e pinheira

    OpenAIRE

    Bruno Monteiro Rodrigues; Bruna Dias Souza; Rejane Mansur Nogueira; Mauro Guida Santos

    2010-01-01

    The predawn leaf water potential (Ψl), stomatal conductance (g s), CO2 assimilation (A), transpiration (E), chlorophyll a fluorescence and leaf metabolite contents (soluble sugars, proteins and free amino acids) of two tropical fruit species grown in a greenhouse were evaluated to determine the effect of induced water stress on young plants. Six month-old jackfruit (Artocarpus heterophyllus Lam.) and sugar apple (Annona squamosa L.) plants were evaluated in 10.0 L pots after eight days o...

  15. Warning: safety risk with some Apple AC Wall Plug Adapters

    CERN Multimedia

    CERN IT department

    2016-01-01

    Dear Mac and iOS Users, Apple has determined that some of its two prong Apple AC wall plug adapters may break and create a risk of electrical shock.   CERN users can now exchange their affected Apple wall plug adapters at the Service Desk. To find out if your adapter is affected and for any further information concerning the procedure to follow to exchange it, please check the following URL: https://cern.service-now.com/service-portal/view-outage.do?n=OTG0028639.

  16. Analysis of Ultrasonic Transmitted Signal for Apple using Wavelet Transform

    International Nuclear Information System (INIS)

    This study was conducted to analyze the ultrasonic transmitted signal for apple using wavelet transform. Fruit consists of nonlinear visco-elastic properties such as flesh, an ovary and rind and lienee most ultrasonic wave is attenuated and its frequency is shifted during passing the fruit. Thus it is not easy to evaluate the internal quality of the fruit using typical ultrasonic parameters such as wave velocity, attenuation, and frequency spectrum. The discrete wavelet transform was applied to the ultrasonic transmitted signal for apple. The magnitude of the first peak frequency of the wavelet basis from the ultrasonic transmitted signal showed a close correlation to the storage time of apple

  17. Traditional versus modern apple cultivars – a comparison of juice composition

    OpenAIRE

    Dobrowolska-Iwanek Justyna; Gąstoł Maciej; Adamska Agnieszka; Krośniak Mirosław; Zagrodzki Paweł

    2015-01-01

    Apples are rich in components that are important for human health, such as polyphenols, organic acids and microelements, but their chemical composition varies with different apple cultivars. We aimed to find out if there is any superiority in traditionally grown apple cultivars in terms of their fruits’ bioactive components content. Juice from two traditionally grown apple cultivars, ‘Mutsu’ and ‘Reinette Simirenko’, was compared to eight popular apple cultivars, ‘Jonagold’, ‘Jonagold Decosta...

  18. Biological Control of Apple Ring Rot on Fruit by Bacillus amyloliquefaciens 9001

    OpenAIRE

    LI Yan; Han, Li-Rong; Zhang, Yuanyuan; Fu, Xuechi; Chen, Xinyi; Zhang, Lixia; Mei, Ruhong; Wang, Qi

    2013-01-01

    Apple ring rot disease, caused by Botryosphaeria dothidea (Moug. ex. Fr) Ces. et de Not., is one of the most important diseases on apple fruits. In this study, strain 9001 isolated from healthy apple fruits from an infested orchard was evaluated for its biocontrol activity against apple ring rot in vitro and in vivo. Strain 9001 showed obvious antagonistic activity to B. dothidea YL-1 when plated on potato dextrose agar. Soaking healthy apples in the bacterial suspensions of strain 9001 prior...

  19. Demand Estimation for US Apple Juice Imports: A Restricted Source Differentiated AIDS Model

    OpenAIRE

    Mekonnen, Dawit Kelemework; Fonsah, Esendugue Greg

    2011-01-01

    Although this paper focuses on apple juice, a restricted version of source differentiated Almost Ideal Demand System (RSDAIDS) was used to examine U.S. import demand for fresh apple, apple juice and other processed apple. Apple imports were differentiated by type and source of origin and the RSDAIDS model was estimated after imposing the general demand restrictions of adding-up, homogeneity and slutsky symmetry. Seasonality and trend variables were also included on the model. The estimation r...

  20. Analysis of naltrexone urinary metabolites.

    Science.gov (United States)

    Ventura, R; de la Torre, R; Segura, J

    1988-01-01

    A reversed-phase HPLC method using ion-pair formation has been developed for the simultaneous determination of naltrexone and three urinary metabolites. The extraction of the free and conjugated metabolites was studied by liquid-solid procedures using styrene-divinylbenzene copolymers (Amberlite XAD-2) and bonded octadecyl silica supports (ODS-silica). Optimum recovery was obtained with ODS-silica extraction using 25% acetonitrile in a 5 mM diammonium phosphate buffer pH 2.1 as elution solvent. The chromatographic behaviour of naltrexone metabolites and naloxone (internal standard) was examined by varying the mobile phase composition. Increments of both the diammonium phosphate buffer concentration and the percentage of organic solvent in the eluent decreases the retention of compounds in a non-linear manner. Increments of the dodecyl sulphate (counter-ion) concentration, increases the retention time. The method was applied to determine the urinary levels of major naltrexone metabolites in a volunteer receiving a 50 mg oral dose. This is the first method reported which permits the simultaneous quantitative determination of naltrexone and its metabolites, 6beta-naltrexol, naltrexone glucuronide and 6beta-naltrexol glucuronide, in urine. PMID:16867358

  1. Cell biology apps for Apple devices.

    Science.gov (United States)

    Stark, Louisa A

    2012-01-01

    Apps for touch-pad devices hold promise for guiding and supporting learning. Students may use them in the classroom or on their own for didactic instruction, just-in-time learning, or review. Since Apple touch-pad devices (i.e., iPad and iPhone) have a substantial share of the touch-pad device market (Campbell, 2012), this Feature will explore cell biology apps available from the App Store. My review includes iPad and iPhone apps available in June 2012, but does not include courses, lectures, podcasts, audiobooks, texts, or other books. I rated each app on a five-point scale (1 star = lowest; 5 stars = highest) for educational and production values; I also provide an overall score. PMID:22949420

  2. Photoprotective effects of apple peel nanoparticles

    Directory of Open Access Journals (Sweden)

    Bennet D

    2013-12-01

    Full Text Available Devasier Bennet,1 Se Chan Kang,2 Jongback Gang,3 Sanghyo Kim1,4 1Department of Bionanotechnology, 2Department of Life Science, 3Department of Nano Chemistry, Gachon University, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do, Republic of Korea; 4Graduate Gachon Medical Research Institute, Gil Medical Center, Inchon, Republic of Korea Abstract: Plants contain enriched bioactive molecules that can protect against skin diseases. Bioactive molecules become unstable and ineffective due to unfavorable conditions. In the present study, to improve the therapeutic efficacy of phytodrugs and enhance photoprotective capability, we used poly(D,L-lactide-co-glycolide as a carrier of apple peel ethanolic extract (APETE on permeation-enhanced nanoparticles (nano-APETE. The in vitro toxicity of nano-APETE-treated dermal fibroblast cells were studied in a bioimpedance system, and the results coincided with the viability assay. In addition, the continuous real-time evaluations of photodamage and photoprotective effect of nano-APETE on cells were studied. Among three different preparations of nano-APETE, the lowest concentration provided small, spherical, monodispersed, uniform particles which show high encapsulation, enhanced uptake, effective scavenging, and sustained intracellular delivery. Also, the nano-APETE is more flexible, allowing it to permeate through skin lipid membrane and release the drug in a sustained manner, thus confirming its ability as a sustained transdermal delivery. In summary, 50 µM nano-APETE shows strong synergistic photoprotective effects, thus demonstrating its higher activity on target sites for the treatment of skin damage, and would be of broad interest in the field of skin therapeutics. Keywords: apple peel ethanolic extract, antioxidant, cellular uptake, electric cell-substrate impedance sensing, phyto-drugs, light-induced damage

  3. The association of Tarsonemus mites (Acari: Heterostigmata) with different apple developmental stages and apple core rot diseases

    OpenAIRE

    UECKERMANN, Edward Albert; Van der Walt, Lené; Spotts, Robert A.; Smit, Francois J.; Jensen, Tamaryn; McLeod, Adéle

    2011-01-01

    Information on the role of mites in the genus Tarsonemus Canestrini and Fanzago, 1876 in the epidemiology of apple core rots (wet and dry) is limited. The aims of this study were to (1) assess the effect of different apple developmental stages (buds, blossoms, 4-cm diameter fruit, mature fruit and mummies) on the relative abundance of Tasonemus mites, (2) determine if there is a tendency of Tarsonemus mites to be associated with wet core rot (WCR) and dry core rot (DCR) apples, and (3) evalua...

  4. Different Polar Metabolites and Protein Profiles between High- and Low-Quality Japanese Ginjo Sake.

    Directory of Open Access Journals (Sweden)

    Kei Takahashi

    Full Text Available Japanese ginjo sake is a premium refined sake characterized by a pleasant fruity apple-like flavor and a sophisticated taste. Because of technical difficulties inherent in brewing ginjo sake, off-flavors sometimes occur. However, the metabolites responsible for off-flavors as well as those present or absent in higher quality ginjo sake remain uncertain. Here, the relationship between 202 polar chemical compounds in sake identified using capillary electrophoresis coupled with time-of-flight mass spectrometry and its organoleptic properties, such as quality and off-flavor, was examined. First, we found that some off-flavored sakes contained higher total amounts of metabolites than other sake samples. The results also identified that levels of 2-oxoglutaric acid and fumaric acid, metabolites in the tricarboxylic acid cycle, were highly but oppositely correlated with ginjo sake quality. Similarly, pyridoxine and pyridoxamine, co-enzymes for amino transferase, were also highly but oppositely correlated with ginjo sake quality. Additionally, pyruvic acid levels were associated with good quality as well. Compounds involved in the methionine salvage cycle, oxidative glutathione derivatives, and amino acid catabolites were correlated with low quality. Among off-flavors, an inharmonious bitter taste appeared attributable to polyamines. Furthermore, protein analysis displayed that a diversity of protein components and yeast protein (triosephosphate isomerase, TPI leakage was linked to the overall metabolite intensity in ginjo sake. This research provides insight into the relationship between sake components and organoleptic properties.

  5. Storability evaluation of Golab apple with acoustic and penetration methods

    Directory of Open Access Journals (Sweden)

    M. R Bayati

    2016-04-01

    Full Text Available Introduction: Apple fruit (Mauls domestica Borkh, Rosaceae after citrus fruits, grape and banana, is the fourth important fruit in the world and is considered the most important fruit of temperate regions. In terms of trade volume, Iran is fourth producer and 17th exporter in the world. Among Iranian cultivars of apple fruit, known as “Golab apple”. Golab apple is one of the fragrant and tasty varieties and meanwhile is very sensitive and also its period of the postharvest shelf life is very short. In a study, the firmness of pear fruit during 4 weeks of storage was monitored using non-destructive impulse response (I-R and destructive Magness-Taylor (M-T puncture tests. The results of this study showed that the dominant frequency, stiffness coefficient and elasticity coefficient as a function of time could be expressed as a decreasing linear function (Gómez et al., 2005. Tiplica et al., (2010, showed that acoustic measurement can be a useful tool to discriminate different apple batches with a low error rate. Starting from the spectrum of the signal recorded by a microphone after the impact of a small hammer on the fruit, 18 key features were identified and used for the classification of apples belonging to 10 different varieties. The study aimed to evaluate apple firmness measured using both the penetrometer and acoustic methods. The methodologies were applied to Royal Gaya and Golden Smoothee apples harvested from 12 different orchards in Catalonia (Spain, on six different dates, and over three seasons. The results obtained showed a noticeable correlation between Magness Taylor firmness and acoustic measurements in Royal Gala, but no correlation was found for Golden Smoothee. In this study, also, acoustic measurements seemed to be a good tool for evaluating changes in tissue firmness during long-term storage (Molina-Delgado et al., 2009. In another study, it was presented a novel approach based on the simultaneous profiling of the

  6. Clarification Effects of Chitosan on Apple Fruit Juice

    Institute of Scientific and Technical Information of China (English)

    WANG Hong-fei; LI He-sheng; ZHANG Xiao-ping; HUANG Xiao-chun

    2003-01-01

    Chitosan is a good flocculant. The paper deals with the clarification of apple juice by means of chitosan. The results showed that the transmittance was over 97% and soluble solid content was stable basically, under the technological condition of chitosan of 0.5 - 1.2 g L-1 , temperature of 45 - 55℃ and pH 4.5. After the orthogonal trial, the optimum technological conditions of apple juice clarification by using chitosan were 0.3 g L-1 chitosan, 45℃C and pH 4.5. The research of the apple juice clarification with chitosan made a basis of the application of chitosan and provided the theoretical basis for the clarification of apple juice with chitosan.

  7. Apple tree production in Italy: rootstocks, cultivars, fertilization, and irrigation

    Directory of Open Access Journals (Sweden)

    Giovambattista Sorrenti

    2012-11-01

    Full Text Available Italy is one of the main apple producers in Europe, primarily intended for fresh consumption, both in the domestic and foreign markets. Fruit yield and quality depends on the cultivar, rootstock, and management practices, such as the fertilization and irrigation adopted in the orchard. This review aims at reporting the main apple cultivars and rootstocks, the management of fertilization and irrigation, as well as their adaptation to apple tree orchards in Italy. The programs for genetic improvement carried out in this country involved the selection of apple tree cultivars and rootstocks which enable a high fruit yield and quality, in order to meet the requirements from the consumer market. In the fertilization and irrigation management, nutrients and water are supplied in amounts next to the actual need of the plants, providing an adequate nutrition, a satisfactory yield, and high quality fruits, besides preventing, whenever possible, nutrients and water losses in the environment.

  8. Radiation measurement of apples produced in Aomori prefecture

    International Nuclear Information System (INIS)

    The activities of seven kinds of apples produced in Aomori prefecture were measured with a high pure Ge detector set in a 15 cm-thick lead shield. The activities of 40K were detected in all samples, but an artificial radioactive nuclide was not detected. The slices of an apple were contacted to an imaging plate for 15 days in a 15 cm-thick lead shield and cross sectional activity distributions were measured. By using the distribution map, the activities of skin, flesh and lead portion of an apple 'Ohrin' were calculated to 0.041, 0.032 and 0.046 Bq/g, respectively. This measuring system will be used for the activity estimations of the farm products, specially apples, because of that many atomic institutions are in operation and in the planning stage on Aomori prefecture. (author)

  9. Food allergy to apple and specific immunotherapy with birch pollen

    DEFF Research Database (Denmark)

    Hansen, Kirsten Skamstrup; Khinchi, Marianne Søndergaard; Skov, Per Stahl;

    2004-01-01

    Conflicting results concerning the effect of specific pollen immunotherapy (SIT) on allergy to plant foods have been reported. The aim of this study was to investigate the effect of SIT using a birch pollen extract on food allergy with focus on allergy to apple. Seventy-four birch pollen...... (SLIT), and 8 (placebo) patients after treatment compared to 10, 4, and 10 patients, respectively, before SIT. The symptom scores to apple during challenges decreased in all groups, but only significantly in the placebo group (p = 0.03). As evaluated by the questionnaire, the severity of food allergy in......-allergic patients were included in a double-blind, double-dummy, and placebo-controlled comparison of sublingual-swallow (SLIT) and subcutaneous (SCIT) administration of a birch pollen extract. Sixty-nine percent of these patients reported allergy to apple. The clinical reactivity to apple was evaluated by open...

  10. Apple Can Act as Anti-Aging on Yeast Cells

    Directory of Open Access Journals (Sweden)

    Vanessa Palermo

    2012-01-01

    Full Text Available In recent years, epidemiological and biochemical studies have shown that eating apples is associated with reduction of occurrence of cancer, degenerative, and cardiovascular diseases. This association is often attributed to the presence of antioxidants such as ascorbic acid (vitamin C and polyphenols. The substances that hinder the presence of free radicals are also able to protect cells from aging. In our laboratory we used yeast, a unicellular eukaryotic organism, to determine in vivo efficacy of entire apples and their components, such as flesh, skin and polyphenolic fraction, to influence aging and oxidative stress. Our results indicate that all the apple components increase lifespan, with the best result given by the whole fruit, indicating a cooperative role of all apple components.

  11. AppleTalk Routing: Phase-Out 30 September 2005

    CERN Multimedia

    Communications Systems Group / IT

    2005-01-01

    AppleTalk is a legacy MacOS protocol for discovering network file, print and other services. IP (Internet Protocol) has become a global standard for networking, replacing non-IP networks, like DECnet, IPX, AppleTalk and others. Following this evolution, support for AppleTalk on the CERN network infrastructure ceases on 30 September 2005. The replacement solutions are: SMB or HTTP for file services LPR for print services. We invite all users who haven't done so already to switch to the replacement solutions. Instructions on how to do so are on the Web at: http://cern.ch/it/gencomputing/mac-support/AppleTalk.htm IT/Communications Systems Group

  12. AppleTalk Routing: Phase-Out 30 September 2005

    CERN Multimedia

    IT/Communications Systems Group

    2005-01-01

    AppleTalk is a legacy MacOS protocol for discovering network file, print and other services. IP (Internet Protocol) has become a global standard for networking, replacing non-IP networks, like DECnet, IPX, AppleTalk and others. Following this evolution, support for AppleTalk on the CERN network infrastructure ceases on 30 September 2005. The replacement solutions are: * SMB or HTTP for file services * LPR for print services. We invite all users who haven't done so already to switch to the replacement solutions. Instructions on how to do so are on the Web at: http://cern.ch/it/gencomputing/mac-support/AppleTalk.htm IT/Communications Systems Group

  13. Protective effects of Egyptian cloudy apple juice and apple peel extract on lipid peroxidation, antioxidant enzymes and inflammatory status in diabetic rat pancreas

    OpenAIRE

    Fathy, Samah M.; Drees, Ehab A.

    2016-01-01

    Background Apples possess rich content of varied polyphenolic compounds showing a variety of biological activities that may ascribe to worthy effects against some chronic diseases. The present study was designed to assess the protective effects of the cloudy apple juice (CAJ) and apple peel extract (APE) of Egyptian Anna apple on the complications in experimental diabetes. Materials and methods Four groups were studied. Diabetes was induced by a single dose of streptozotocin (STZ) to only thr...

  14. Storage test on apple juice after ultrasound treatment

    OpenAIRE

    Filomena Montemurro; Luca Fasolato; Stefania Balzan; Roberta De Nardi; Giorgio Marchesini; Barbara Cardazzo; Enrico Novelli

    2014-01-01

    Apple juice, for its sensory and nutritional qualities, is consumed by people of all ages. Apples are an excellent source of several phenolic compounds and the presence of polyphenols is recognized for their health promoting antioxidant properties. Thermal pasteurization of fruit juices is the conventional method used for their preservation. Therefore, this constitutes the most extensively available methods for the inactivation of microorganisms in fruit juices but it causes side effects on t...

  15. Pectin methyl esterase activity in apple and orange pulps

    International Nuclear Information System (INIS)

    The results of pectin methyl esterase activity from apple, orange pulp and orange peel depending of ph and temperature are discussed. It's shown that the methyl esterase activity form apple and orange pulps higher in range of temperatures from +37...+60digC. The analysis of dependence of its activity from ph has shown that in both case the enzyme activity increase with increase of ph

  16. SPECIES COMPOSITION OF WEED VEGETATION IN DIFFERENT APPLE GROWING TECHNOLOGIES

    OpenAIRE

    Venera TASSEVA

    2005-01-01

    The investigation was carried out in the period 2001-2003 in an orchard of the Institute of Agriculture, Kyustendil, Bulgaria, created in the spring of 1996 on leached cinnamonic forest soil. The weed populations under four different farming technologies of growing of apple cultivar Florina were investigated. It was established, that the apple growing technologies influence the weed association composition. The highest weed diversity was found in the organic technology - 16 weed species were ...

  17. Current progress in trans- and cisgenic apple and strawberry Breeding

    OpenAIRE

    Krens, F.A.; Salentijn, E.M.J.; Schaart, J.; Schouten, H J; Jacobsen, E.

    2012-01-01

    A summary is presented of the state-of-the-art in apple and strawberry biotechnological research going on in the department of Plant Breeding at Wageningen University and Research Centre. In apple, the research directed towards the introduction of scab resistance by inserting a barley gene has reached the stage of a field trial where performance of transgenic lines could be tested in an orchard situation. The development of a marker-free system allowing the removal of undesired sequences when...

  18. Hydrolysis-extraction of apple proto pectins in dynamic mode

    International Nuclear Information System (INIS)

    The article describes a hydrolysis process of apple husks by using dynamics regime of hydrolysis. It's shown that application of dynamics method positively influences on the pectin yields and its main parameters. It was defined that by dynamics regime of hydrolysis-extraction of apple husks it is possible to obtain qualitative products with high yield at a mild ph value of medium of hydrolysing agent.

  19. Impact of Apple Pomace on the Property of French Bread

    OpenAIRE

    Yaqiang He; Qian Lu

    2015-01-01

    This study analyzed properties of wheat dough and French bread added with different kinds of apple pomace. In the analysis of wheat dough, both farinograph indexes and extenograph indexes were used as evaluation criteria. Specific volume, whiteness and bread core pores were employed to evaluate the property of French bread. Analysis on the nutrient profile of apple pomace indicated that the content of dietary fiber could reach 15.8%. The result of farinograph indexes and extenograph indexes s...

  20. Sustainable apple breedings needs sustainable marketing and management

    OpenAIRE

    Weber, M.

    2008-01-01

    Apple breeding programmes are currently in the middle of transition in terms of ownership and management. Until now most of them were funded by the public. Breeding took place by traditional methods since decades in a very sustainable way to develop better apple varieties. Today, increasing loss of national boundaries and globalisation, less interest by national bodies and institutions and rising cost levels for high tech breeding methods entire programmes are nowadays urged to...

  1. Characterization of polyphenoloxidase (PPO) extracted from "Jonagored" apple

    OpenAIRE

    Rocha, A. M. C. N.; Morais, A. M. M. B.

    2001-01-01

    Polyphenoloxidase (PPO) was extracted from apple (cv. Jonagored) with addition of 2% PVP and 0.25% Triton X100 to the extraction buffer containing phenolic adsorbents. Experiments were performed to evaluate the affinity and specificity towards several substrates. ‘Jonagored’ apple PPO was found to have higher specificity (lower Km) towards L-dopa, 4-methylcatechol and (+) catechin than other phenols tested, but the highest activity level was obtained with p-cresol. The ratio Vmax/Km indicates...

  2. Outsourcing and corporate social responsibility : Apple in China

    OpenAIRE

    Urakami, Kiyoshi

    2012-01-01

    Electronics industry has seen a tremendous industry shift from the developed countries to the emerging regions such as East and South Asia, particularly during the past four decades. And we can now see a huge industrial capability accumulation in Asia. This research note aims at, firstly, describing basic nature and structure of outsourcing business in Asia, and, secondly, we look into Apple Inc.'s supply chain to examine Apple supplier factory operations in Mainland China from, primarily,...

  3. Kui suudab Steve, suudab ka Apple / Tarvo Vaarmets

    Index Scriptorium Estoniae

    Vaarmets, Tarvo

    2008-01-01

    Ehkki Apple'i kolmanda kvartali kasum kasvas kolmandiku võrra ja oli oodatust kümnendiku jagu suurem, langes ettevõtte aktsia hind börsil. Investoreid teeb rahutuks nii ettevõtte juhi Steve Jobsi tervis kui ka Apple'i majandusprognoos neljandaks kvartaliks. Lisa: Analüütikud on aktsia suhtes üksmeelel. Diagramm: Aktsia hinnal taas suund alla

  4. Mealiness assessment in apples and peaches using MRI techniques

    OpenAIRE

    Barreiro Elorza, Pilar; Ortiz, Christopher; Ruiz-Altisent, Margarita; Ruiz Cabello Osuna, Jesus Maria; Fernandez Valle, M. Encarnacion; Recasens, I.; Asensio, M

    2000-01-01

    Mealiness (woolliness in peaches) is a negative attribute of sensory texture that combines the sensation of a desegregated tissue with the sensation of lack of juiciness. In this study, 24 apples cv. Top Red and 8 peaches cv. Maycrest, submitted to 3 and 2 different storage conditions respectively have been tested by mechanical and MRI techniques to assess mealiness. With this study, the results obtained on apples in a previous work have been validated using mathematical features from the his...

  5. Apple Puree-Alginate Edible Coating as Carrier of Antimicrobial Agents to Prolong Shelf-Life of Fresh-Cut Apples

    Science.gov (United States)

    Edible coatings with antimicrobial agents can extend the shelf-life of fresh-cut fruit. The effect of lemongrass, oregano oil and vanillin incorporated in apple puree-alginate edible coatings, on the shelf-life of fresh-cut Fuji apples, was investigated. Coated apples were packed in air-filled pol...

  6. Blank Computer Floppy Disk Formatting Using the AppleWorks Program, Apple IIe or GS Computers and a Duodisk or Two Disk Drives.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for formatting blank floppy disks in the AppleWorks program using an Apple IIe or Apple IIGS Computer with Duodisk or two disk drives. The manual provides step-by-step directions, and includes 11 figures depicting the computer screen at the various stages of the formatting sequence. (EW)

  7. Developing Simple Grade Books Using the AppleWorks Spreadsheet Subprogram, Apple IIe or GS Computers, and a Duodisk or Two Disk Drives.

    Science.gov (United States)

    Schlenker, Richard M.

    This manual is a "how to" training device for developing grade books using the AppleWorks spreadsheet subprogram with an Apple IIe or Apple IIGS Computer which has a Duodisk or two disk drives and an 80-column card. The manual provides step-by-step directions, and includes 41 figures depicting the computer screen at the various stages of the…

  8. Modeling apple surface temperature dynamics based on weather data.

    Science.gov (United States)

    Li, Lei; Peters, Troy; Zhang, Qin; Zhang, Jingjin; Huang, Danfeng

    2014-01-01

    The exposure of fruit surfaces to direct sunlight during the summer months can result in sunburn damage. Losses due to sunburn damage are a major economic problem when marketing fresh apples. The objective of this study was to develop and validate a model for simulating fruit surface temperature (FST) dynamics based on energy balance and measured weather data. A series of weather data (air temperature, humidity, solar radiation, and wind speed) was recorded for seven hours between 11:00-18:00 for two months at fifteen minute intervals. To validate the model, the FSTs of "Fuji" apples were monitored using an infrared camera in a natural orchard environment. The FST dynamics were measured using a series of thermal images. For the apples that were completely exposed to the sun, the RMSE of the model for estimating FST was less than 2.0 °C. A sensitivity analysis of the emissivity of the apple surface and the conductance of the fruit surface to water vapour showed that accurate estimations of the apple surface emissivity were important for the model. The validation results showed that the model was capable of accurately describing the thermal performances of apples under different solar radiation intensities. Thus, this model could be used to more accurately estimate the FST relative to estimates that only consider the air temperature. In addition, this model provides useful information for sunburn protection management. PMID:25350507

  9. Modeling Apple Surface Temperature Dynamics Based on Weather Data

    Directory of Open Access Journals (Sweden)

    Lei Li

    2014-10-01

    Full Text Available The exposure of fruit surfaces to direct sunlight during the summer months can result in sunburn damage. Losses due to sunburn damage are a major economic problem when marketing fresh apples. The objective of this study was to develop and validate a model for simulating fruit surface temperature (FST dynamics based on energy balance and measured weather data. A series of weather data (air temperature, humidity, solar radiation, and wind speed was recorded for seven hours between 11:00–18:00 for two months at fifteen minute intervals. To validate the model, the FSTs of “Fuji” apples were monitored using an infrared camera in a natural orchard environment. The FST dynamics were measured using a series of thermal images. For the apples that were completely exposed to the sun, the RMSE of the model for estimating FST was less than 2.0 °C. A sensitivity analysis of the emissivity of the apple surface and the conductance of the fruit surface to water vapour showed that accurate estimations of the apple surface emissivity were important for the model. The validation results showed that the model was capable of accurately describing the thermal performances of apples under different solar radiation intensities. Thus, this model could be used to more accurately estimate the FST relative to estimates that only consider the air temperature. In addition, this model provides useful information for sunburn protection management.

  10. Natural products: Hunting microbial metabolites

    Science.gov (United States)

    Schmidt, Eric W.

    2015-05-01

    Symbiotic bacteria synthesize many specialized small molecules; however, establishing the role these chemicals play in human health and disease has been difficult. Now, the chemical structure and mechanism of the Escherichia coli product colibactin provides insight into the link between this secondary metabolite and colorectal cancer.

  11. Primary expectations of secondary metabolites

    Science.gov (United States)

    Plant secondary metabolites (e.g., phenolics) are important for human health, in addition to the organoleptic properties they impart to fresh and processed foods. Consumer expectations such as appearance, taste, or texture influence their purchasing decisions. Thorough identification of phenolic com...

  12. The secondary metabolite bioinformatics portal

    DEFF Research Database (Denmark)

    Weber, Tilmann; Kim, Hyun Uk

    2016-01-01

    . In this context, this review gives a summary of tools and databases that currently are available to mine, identify and characterize natural product biosynthesis pathways and their producers based on ‘omics data. A web portal called Secondary Metabolite Bioinformatics Portal (SMBP at http...

  13. Effects of Apple Juice Concentrate, Blackcurrant Concentrate and Pectin Levels on Selected Qualities of Apple-Blackcurrant Fruit Leather

    OpenAIRE

    Diamante, Lemuel M.; Siwei Li; Qianqian Xu; Janette Busch

    2013-01-01

    A study was conducted to determine the effects of different levels of apple juice concentrate (AJC), blackcurrant concentrate (BCC) and pectin on the moisture content, water activity, color, texture and ascorbic acid content of apple-blackcurrant fruit leather using the response surface methodology. The results showed the moisture content increased with increasing pectin level and with greater increases at higher AJC and BCC levels while the water activity increased with increasing pectin lev...

  14. Effects of Microwave Pretreatment of Apple Raw Material on the Nutrients and Antioxidant Activities of Apple Juice

    OpenAIRE

    Shaoying Zhang; Rui Zhang

    2014-01-01

    Microwave technology has been widely applied in food processing. To investigate the effects of microwave pretreatment of raw material on the nutrients and antioxidant activities of apple juice, the apple materials were treated with 90, 270, 450, 720, and 900 W microwave at 25, 50, 75, 100, and 125 s, respectively. The results showed that after the raw materials were treated with microwave, the vitamin C, amino nitrogen, and anthocyanin content decreased, and the total flavonoids and polypheno...

  15. CLIPS, AppleEvents, and AppleScript: Integrating CLIPS with commercial software

    Science.gov (United States)

    Compton, Michael M.; Wolfe, Shawn R.

    1994-01-01

    Many of today's intelligent systems are comprised of several modules, perhaps written in different tools and languages, that together help solve the user's problem. These systems often employ a knowledge-based component that is not accessed directly by the user, but instead operates 'in the background' offering assistance to the user as necessary. In these types of modular systems, an efficient, flexible, and eady-to-use mechanism for sharing data between programs is crucial. To help permit transparent integration of CLIPS with other Macintosh applications, the AI Research Branch at NASA Ames Research Center has extended CLIPS to allow it to communicate transparently with other applications through two popular data-sharing mechanisms provided by the Macintosh operating system: Apple Events (a 'high-level' event mechanism for program-to-program communication), and AppleScript, a recently-released scripting language for the Macintosh. This capability permits other applications (running on either the same or a remote machine) to send a command to CLIPS, which then responds as if the command were typed into the CLIPS dialog window. Any result returned by the command is then automatically returned to the program that sent it. Likewise, CLIPS can send several types of Apple Events directly to other local or remote applications. This CLIPS system has been successfully integrated with a variety of commercial applications, including data collection programs, electronics forms packages, DBMS's, and email programs. These mechanisms can permit transparent user access to the knowledge base from within a commercial application, and allow a single copy of the knowledge base to service multiple users in a networked environment.

  16. Trace Elements in Apple Fruits of Several Regions in the Republic of Macedonia

    International Nuclear Information System (INIS)

    Determination of macro elements and trace elements in apple fruits was carried out by ICP-AES method. Apples were picked up fresh in December 2005. Seven different types of apples from different parts of the country were selected. Apples were sliced into large pieces, dried, and after grinding were dissolved in nitric acid (HNO3) and hydroperoxide (H2O2). After digestion, multi-element analysis was carried out. Data obtained were compared to published values for trace elements in apples in other regions of the world. Data obtained indicated that the concentration of trace elements in apples were within the values and data in other regions of the world. (Author)

  17. Identification of Epoxide-Derived Metabolite(s) of Benzbromarone.

    Science.gov (United States)

    Wang, Kai; Wang, Hui; Peng, Ying; Zheng, Jiang

    2016-04-01

    Benzbromarone (BBR) is a benzofuran derivative that has been quite useful for the treatment of gout; however, it was withdrawn from European markets in 2003 because of reported serious incidents of drug-induced liver injury. BBR-induced hepatotoxicity has been suggested to be associated with the formation of a quinone intermediate. The present study reported epoxide-derived intermediate(s) of BBR. An N-acetylcysteine (NAC) conjugate derived from epoxide metabolite(s) was detected in both microsomal incubations of BBR and urine samples of mice treated with BBR. The NAC conjugate was identified as 6-NAC BBR. Ketoconazole suppressed the bioactivation of BBR to the epoxide intermediate(s), and the CYP3A subfamily was the primary enzyme responsible for the formation of the epoxide(s). The present study provided new information on metabolic activation of BBR. PMID:26792818

  18. STUDY ON THE MACRONUTRIENT CONTENT OF APPLE LEAVES IN AN ORGANIC APPLE ORCHARD

    Directory of Open Access Journals (Sweden)

    Péter NAGY

    2006-12-01

    Full Text Available Macronutrient contents of soil and apple leaves were investigated in an organic apple orchard in Eastern Hungary in 2002-2004. Soil samples were taken from 0-20 cm depth in April and October. The macronutrient content of leaves was measured on cvs. Jonagold, Mutsu, Idared, Red Elstar, Egri Piros, Reka, and Remo at six assessment dates (from April to September. The macronutrient contents of N, P, K, S, Ca and Mg were measured in both soil and leaf samples, then macronutrient ratios were calculated from the obtained results. The results showed that younger leaves contained more N and P than older ones. K and Ca contents of leaves decreased until July, then increased slightly, and decreased again. A continuous decrease of the S content of leaves was observed until August. The Mg content of leaves increased until June, then decreased in July and then increased again. Macronutrient values were dependent on cultivar. Calculated macronutrient ratios showed that the nutrient supply of soil was not optimal in the orchard.

  19. Secondary Metabolites from Rubiaceae Species

    OpenAIRE

    Daiane Martins; Cecilia Veronica Nunez

    2015-01-01

    This study describes some characteristics of the Rubiaceae family pertaining to the occurrence and distribution of secondary metabolites in the main genera of this family. It reports the review of phytochemical studies addressing all species of Rubiaceae, published between 1990 and 2014. Iridoids, anthraquinones, triterpenes, indole alkaloids as well as other varying alkaloid subclasses, have shown to be the most common. These compounds have been mostly isolated from the genera Uncaria, Psych...

  20. Autism and Phthalate Metabolite Glucuronidation

    OpenAIRE

    Stein, T. Peter; Schluter, Margaret D.; Steer, Robert A.; Ming, Xue

    2013-01-01

    Exposure to environmental chemicals may precipitate autism spectrum disorders (ASD) in genetically susceptible children. Differences in the efficiency of the glucuronidation process may substantially modulate substrate concentrations and effects. To determine whether the efficiency of this pathway is compromised in children with ASD, we measured the efficiency of glucuronidation for a series of metabolites derived from the commonly used plasticizer, diethylhexyl phthalate. Spot urines were co...

  1. Epigenome targeting by probiotic metabolites

    OpenAIRE

    Licciardi Paul V; Wong Sook-San; Tang Mimi LK; Karagiannis Tom C

    2010-01-01

    Abstract Background The intestinal microbiota plays an important role in immune development and homeostasis. A disturbed microbiota during early infancy is associated with an increased risk of developing inflammatory and allergic diseases later in life. The mechanisms underlying these effects are poorly understood but are likely to involve alterations in microbial production of fermentation-derived metabolites, which have potent immune modulating properties and are required for maintenance of...

  2. MdMYB1 Regulates Anthocyanin and Malate Accumulation by Directly Facilitating Their Transport into Vacuoles in Apples.

    Science.gov (United States)

    Hu, Da-Gang; Sun, Cui-Hui; Ma, Qi-Jun; You, Chun-Xiang; Cheng, Lailiang; Hao, Yu-Jin

    2016-03-01

    Tonoplast transporters, including proton pumps and secondary transporters, are essential for plant cell function and for quality formation of fleshy fruits and ornamentals. Vacuolar transport of anthocyanins, malate, and other metabolites is directly or indirectly dependent on the H(+)-pumping activities of vacuolar H(+)-ATPase (VHA) and/or vacuolar H(+)-pyrophosphatase, but how these proton pumps are regulated in modulating vacuolar transport is largely unknown. Here, we report a transcription factor, MdMYB1, in apples that binds to the promoters of two genes encoding the B subunits of VHA, MdVHA-B1 and MdVHA-B2, to transcriptionally activate its expression, thereby enhancing VHA activity. A series of transgenic analyses in apples demonstrates that MdMYB1/10 controls cell pH and anthocyanin accumulation partially by regulating MdVHA-B1 and MdVHA-B2. Furthermore, several other direct target genes of MdMYB10 are identified, including MdVHA-E2, MdVHP1, MdMATE-LIKE1, and MdtDT, which are involved in H(+)-pumping or in the transport of anthocyanins and malates into vacuoles. Finally, we show that the mechanism by which MYB controls malate and anthocyanin accumulation in apples also operates in Arabidopsis (Arabidopsis thaliana). These findings provide novel insights into how MYB transcription factors directly modulate the vacuolar transport system in addition to anthocyanin biosynthesis, consequently controlling organ coloration and cell pH in plants. PMID:26637549

  3. Stingless bees further improve apple pollination and production

    Directory of Open Access Journals (Sweden)

    Blandina Felipe Viana

    2014-10-01

    Full Text Available The use of Africanised honeybee (Apis mellifera scutellata Lepeletier hives to increase pollination success in apple orchards is a widespread practice. However, this study is the first to investigate the number of honeybee hives ha-1 required to increase the production of fruits and seeds as well as the potential contribution of the stingless bee Mandaçaia (Melipona quadrifasciata anthidioides Lepeletier. We performed tests in a 43-ha apple orchard located in the municipality of Ibicoara (13º24’50.7’’S and 41º17’7.4’’W in Chapada Diamantina, State of Bahia, Brazil. In 2011, fruits from the Eva variety set six seeds on average, and neither a greater number of hives (from 7 to 11 hives ha-1 nor a greater number of pollen collectors at the honeybee hives displayed general effects on the seed number. Without wild pollinators, seven Africanised honeybee hives ha-1 with pollen collectors is currently the best option for apple producers because no further increase in the seed number was observed with higher hive densities. In 2012, supplementation with both stingless bees (12 hives ha-1 and Africanised honeybees (7 hives ha-1 provided higher seed and fruit production than supplementation with honeybees (7 hives ha-1 alone. Therefore, the stingless bee can improve the performance of honeybee as a pollinator of apple flowers, since the presence of both of these bees results in increases in apple fruit and seed number.

  4. BIOLOGICAL ACTIVITY OF APPLE JUICE ENRICHED BY HERBAL EXTRACTS

    Directory of Open Access Journals (Sweden)

    Eva Ivanišová

    2015-02-01

    Full Text Available Herbal phytochemicals have recently become an attractive subject for scientists in many different research areas. The aim of this study was to determine antioxidant activity, total polyphenol and flavonoid content of apple juice enriched by water herbal extracts. Secondary was to evaluate sensory characteristic of enriched apple juice. It was found that applications of water herbal extracts to apple juice increase antioxidant activities, and also total polyphenol and flavonoid content with compare to pure apple juice. The highest biological activities were detected in apple juice with addition of lemon balm (14.42 mg TEAC/L; 84.38 mg TEAC/L; 50.88 mg GAE/L; 36.26 μg QE/L, oregano (14.92 mg TEAC/L; 79.97 mg TEAC/L; 50.51 mg GAE/L; 31.02 μg QE/L and salvia (8.40 mg TEAC/L; 30.40 mg TEAC/L; 23.33 mg GAE/L; 27.67 μg QE/L water extract. Sensorial analysis of samples showed, that enriched juices had better properties for evaluators with compared to pure juice. The aim of this study was also to mention the potential use of medicinal herbs in food industry, because plant bioactive compounds can play an important role in preventing cardiovascular diseases, cancers and reduction inflammatory action.

  5. Multiple plant hormones and cell wall metabolism regulate apple fruit maturation patterns and texture attributes

    Science.gov (United States)

    Molecular events regulating apple fruit ripening and sensory quality are largely unknown. Such knowledge is essential for genomic-assisted apple breeding and postharvest quality management. In this study, a parallel transcriptome profile analysis, scanning electron microscopic (SEM) examination and...

  6. The Influence of Different Apple Based Supplements on the Intestinal Microbiota of Humans

    DEFF Research Database (Denmark)

    Bergström, Anders; Wilcks, Andrea; Ravn-Haren, Gitte;

    restriction diet during the control period, and in the four other periods it was supplied with four different apple based supplements. Between the diets there was a 2-week wash-out period still on the restriction diet. The four apple based supplements were: 1) whole apples, 2) clear apple juice (pectin......Background and objective: The present project is part of the large ISAFRUIT project, where one of the objectives is to identify effects of apple and apple product on parameters related to gut health. In a previous rat study we observed changes in the intestinal microbiota of rats fed whole apples......, pomace or apple pectin ([1], and we were interested in finding out if the same effect can be observed in humans. Method: The study was conducted as a randomized, controlled 5 x 28 days cross-over study with 24 healthy persons of both genders. The persons were following a pectin- and polyphenol free...

  7. Rootstock-regulated gene expression patterns associated with fire blight resistance in apple

    Science.gov (United States)

    Background: Desirable apple varieties are clonally propagated by grafting vegetative scions onto rootstocks. Rootstocks influence many phenotypic traits of the scion, including resistance to pathogens such as Erwinia amylovora, which causes fire blight, the most serious bacterial disease of apple....

  8. Scanning Electron Microscopy Structure and Firmness of Papain Treated Apple Slices

    OpenAIRE

    Luo, Yaguang; Patterson, Max E.; Swanson, Barry G.

    1992-01-01

    'Mcintosh' apple (Malus domesrica Borkh.) slices were treated with papain. Textural changes were recorded with an Instron Universal Testing Machine. Structural changes and distribution of microorganisms in apple tissues after treatment were observed with a scanning electron microscope (SEM). Apple slices submerg ed in a 1% papain solution were significantly firmer than apple slices submerged in the distilled water control for a 24 hour period (P < 0.05). Three and four days after slicing , a ...

  9. Identification of gamma irradiated apples by the half-embryo test

    International Nuclear Information System (INIS)

    The half-embryo test was applied to irradiated apples (var. Red delicious).The irradiation of apples caused obvious changes in the growth of the half-embryo. A dose of 100 Gy or more, inhibits the epicotyl development and with 50 Gy dose is possible to observe a great contrast with the non-irradiated apples. If the epicotyl development is less than 4 cm., the apples are identified as irradiated. The assessment can be made after 7 days. (author)

  10. Characterization of Cider Apples on the Basis of Their Fatty Acid Profiles

    OpenAIRE

    Blanco-Gomis, Domingo; Mangas, J.J. (Juan); Margolles, Inmaculada; Arias, Pilar

    2011-01-01

    In the current study, the fatty acids composition of 30 monovarietal apple juices from six cider apple varieties belonging to two categories was analyzed. The different apple juices were obtained from three consecutive harvests (1997, 1998, and 1999). The fatty acids concentration in apple juice together with chemometric techniques such as principal components analysis (PCA), soft independent modeling of class analogy (SIMCA), and linear discriminant analysis (LDA), allowed us to ...

  11. THE FINANCIAL EFFICIENCY OF THE APPLE PRODUCTION IN BOSNIA AND HERZEGOVINA

    OpenAIRE

    F ĆEJVANOVIĆ; ROVČANIN, A.; Č ROZMAN

    2005-01-01

    General trend of decreasing agricultural land area and global population increase sets new challenges for apple producers. This basically calls for more effi cient apple production. The integrated apple production is based on principles that combine fi nancially viable intensive production and simultaneously consider environment standards could be a solution to emerging problems in apple production. For implementation of this systems new technology standards and orchard management must be app...

  12. Thinking Afresh About Processing: An Exploration of New Market Opportunities for Apple Products

    OpenAIRE

    Rowles, Kristin L.; Henehan, Brian M.; White, Gerald B.

    2001-01-01

    This report compiles the results of several studies focused on the markets for processed apple products. These studies were conducted as part of a multidisciplinary project examining the processing apple industry in the Northeastern United States. The purpose of the studies was to explore the potential for new products and new marketing strategies that might bring innovation to the apple industry. In the global and highly competitive markets in which apple products fight for shelf space and c...

  13. Consumer preferences for organically and locally produced apples

    DEFF Research Database (Denmark)

    Denver, Sigrid; Jensen, Jørgen Dejgård

    2014-01-01

    This study investigates organic consumers’ preferences for local production of apples. The analysis is based on a choice experiment among 637 Danish consumers used in combination with a principal component analysis of a set of opinion questions. The principal component analysis identifies two...... components of questions. Component 1 concerns benefits related to organic products while component 2 relates to positive features of locally produced products. When the components are included in analysis of data from the choice experiment a random parameter error component model suggests that respondents...... who recognize the benefits of organic products have relatively high preferences for both organically and locally produced apples. Respondents who, on the other hand, recognize the benefits of locally produced products have high preferences for domestically and locally produced apples but not for...

  14. Fast Estimation of Dietary Fiber Content in Apple.

    Science.gov (United States)

    Le Gall, Sophie; Even, Sonia; Lahaye, Marc

    2016-02-17

    Dietary fibers (DF) are one of the nutritional benefits of fleshy fruit consumption that is becoming a quality criterion for genetic selection by breeders. However, the AOAC total DF content determination is not readily amenable for screening large fruit collections. A new screening method of DF content in an apple collection based on the automated preparation of cell wall material as an alcohol-insoluble residue (AIR) is proposed. The yield of AIR from 27 apple genotypes was compared with DF measured according to AOAC method 985.29. Although residual protein content in AIRs did not affect DF measurement, subtraction of starch content above 3% dry weight in AIRs was needed to agree with AOAC measured DF. A fast colorimetric screening of starch in AIR was developed to detect samples needing correction. The proposed method may prove useful for the rapid determination of DF in collections of other fleshy fruit besides apple. PMID:26813795

  15. Evaluation of winter temperatures on apple budbreak using grafted twigs

    Directory of Open Access Journals (Sweden)

    Fernando José Hawerroth

    2013-09-01

    Full Text Available Temperature is the main climate factor related to induction, maintenance and dormancy release in apple (Malus domestica Borkh.. The inadequate chilling exposure in apples causes budbreak problems, resulting in decrease in yield potential. Thus, the knowledge of physiological principles and environmental factors determining the dormancy phenomenon, especially winter temperature effects, it is necessary for the efficient selection of cultivars in a productive region. In addition, it is indispensable to adapt the orchard management aiming to decrease the problems caused by lack chilling during winter. The objective of this study was to evaluate the influence of different thermal conditions during the dormancy period on budbreak of apple cultivars. One-year-old twigs of 'Castel Gala' and 'Royal Gala' cultivars, grafted on M7 rootstock, were submitted to temperatures of 5, 10 and 15ºC for different exposure periods (168; 336; 672; 1,008 and 1,344 hours. After treatments execution, the plants were kept in a greenhouse at 25ºC. Budbreak was quantified when accumulated 3,444; 6,888; 10,332; 13,776; 17,220 and 20,664 GDHºC after temperature treatments. The cultivars responded differently to temperature effect during the winter period. The temperature of 15ºC during winter shows a greater effectiveness on 'Castel Gala' apple budbreak while in the 'Royal Gala' apples the temperatures of 5 and 10ºC show better performance. 'Castel Gala' cultivar (low chilling requirement may supply its physiological necessities, may be capable to budburst, even when subjected to higher temperatures in relation to 'Royal Gala' apples (high chilling requirement.

  16. Ethylene Emission of Apples Treated with 1-Methylcyclopropene During Storage

    Directory of Open Access Journals (Sweden)

    Melnyk Oleksandr

    2014-09-01

    Full Text Available The effect of post-harvest application of 1-methylcyclopropene (1-MCP on ethylene emission in early-winter apple (cv. ‘Calville’, winter (‘Gloster’, ‘Golden Delicious’ and ‘Jonagold’, and late-winter cultivars (‘Granny Smith’, ‘Idared’ and ‘Reinette Simirenko’ w as investigated. The fruits were picked at a commercial harvest time and half of them were treated with 1-MCP (‘SmartFresh™’ 0.068 gm-3 for 24 h at 5 °C. Then both groups of apples were placed in a common storage at the temperature of 2 °C. The intensity of fruit ethylene emission (rate of ethylene production was measured and 1 -MCP inhibition index was calculated. During storage, activity of ethylene emission by control apples of ‘Calville’ and ‘Golden Delicious’ showed typical climacteric pattern, while the intensity of ethylene emission of apples of other cultivars only increased gradually. The change of ethylene activity of early-winter apple cv. ‘Calville’ and winter cv. ‘Jonagold’, both treated with 1-MCP after harvest, had exponential character, especially actively increasing after 3 months of storage. At the same time ethylene emission of 1 -MCP treated fruits of other cultivars changed steadily to a low level and was effectively suppressed during the whole 6-month period of storage in normal atmosphere. The value of inhibition index of fruits treated by 1-MCP confirmed the consistent restoration of the ability to ethylene synthesis and thus to post-harvest ripening of some apples cultivars.

  17. Tulemas on Apple'i järjekordne müügihitt / Tõnis Oja

    Index Scriptorium Estoniae

    Oja, Tõnis, 1957-

    2008-01-01

    Suure tõuke Apple'i aktsiahinnale on andnud iPhone'i turuletulek 2007. aasta sügisel, nüüd on müügile tulemas kolmanda põlvkonna mobiiltelefon iPhone. Vt. samas: Apple'i majandusnäitajad; Pürib digikodu valitsejaks. Diagramm: Apple'i aktsia hind

  18. ONLINE HYPERSPECTRAL LINE-SCAN FLUORESCENCE IMAGING FOR SAFETY INSPECTION OF APPLES

    Science.gov (United States)

    A recently developed fast hyperspectral line-scan imaging system integrated with a commercial apple-sorting machine was evaluated for rapid detection of animal feces matter on apples online. Golden Delicious apples obtained from a local orchard were artificially contaminated with a thin smear of co...

  19. Dumping Low and High Resolution Graphics on the Apple IIe Microcomputer System.

    Science.gov (United States)

    Fletcher, Richard K., Jr.; Ruckman, Frank, Jr.

    This paper discusses and outlines procedures for obtaining a hard copy of the graphic output of a microcomputer or "dumping a graphic" using the Apple Dot Matrix Printer with the Apple Parallel Interface Card, and the Imagewriter Printer with the Apple Super Serial Interface Card. Hardware configurations and instructions for high resolution and…

  20. Apple suutis jälle turgu üllatada / Lauri Matsulevitsh

    Index Scriptorium Estoniae

    Matsulevitsh, Lauri

    2005-01-01

    iPodi pleierite ja Mac-arvutite kasvav müük aitas Apple'i kasumit aastaga viiekordistada 61 miljonilt 320 miljoni dollarini. Diagramm: Tulemused vedasid Apple'i tõusule. Vt. samas: Eesti investorid mängisid Apple'i tõusu peale

  1. Spontaneous radiation from relativistic electrons in a tapered Apple-II undulator

    International Nuclear Information System (INIS)

    This paper presents most properties of radiation from a tapered Apple-II undulator. The study demonstrates that tapering an Apple-II undulator can broaden the harmonic bandwidth and the performance of polarization is also excellent at the broadened energy range. So Apple-II undulator can be tapered to provide more convenience for energy scan experiment. (authors)

  2. Field Evaluation of Apple Rootstocks for Orchard Performance and Fire Blight Resistance

    Science.gov (United States)

    In 2002, apple rootstock trials using three scion cultivars were established at Geneva, NY to evaluate 64 apple (Malus X domestica) rootstocks for horticultural performance and fire blight resistance. Field trials compared several elite Geneva® apple rootstocks, which were bred for tolerance to fir...

  3. APPLE: MEDICINE OF ETERNAL LIFE / ÖLÜMSÜZLÜK ILÂCI ELMA

    OpenAIRE

    Prof. Dr. Esma ŞİMŞEK

    2008-01-01

    Apple which is a medicinal fruit has an importantplace in Turkish culture. It is a symbol of abundance,fertility, eternal life, youth, beauty, strength, health, loveand faith. Apple features in myths, stories, tales, customsand beliefs. This study surveys the place of apple inmarriage, funeral, passage rites, folk medicine, learning alanguage, eternal life.

  4. APPLE: MEDICINE OF ETERNAL LIFE / ÖLÜMSÜZLÜK ILÂCI ELMA

    Directory of Open Access Journals (Sweden)

    Prof. Dr. Esma ŞİMŞEK

    2008-10-01

    Full Text Available Apple which is a medicinal fruit has an importantplace in Turkish culture. It is a symbol of abundance,fertility, eternal life, youth, beauty, strength, health, loveand faith. Apple features in myths, stories, tales, customsand beliefs. This study surveys the place of apple inmarriage, funeral, passage rites, folk medicine, learning alanguage, eternal life.

  5. Choosing between an apple and a chocolate bar: the impact of health and taste labels.

    Directory of Open Access Journals (Sweden)

    Suzanna E Forwood

    Full Text Available Increasing the consumption of fruit and vegetables is a central component of improving population health. Reasons people give for choosing one food over another suggest health is of lower importance than taste. This study assesses the impact of using a simple descriptive label to highlight the taste as opposed to the health value of fruit on the likelihood of its selection. Participants (N=439 were randomly allocated to one of five groups that varied in the label added to an apple: apple; healthy apple; succulent apple; healthy and succulent apple; succulent and healthy apple. The primary outcome measure was selection of either an apple or a chocolate bar as a dessert. Measures of the perceived qualities of the apple (taste, health, value, quality, satiety and of participant characteristics (restraint, belief that tasty foods are unhealthy, BMI were also taken. When compared with apple selection without any descriptor (50%, the labels combining both health and taste descriptors significantly increased selection of the apple ('healthy & succulent' 65.9% and 'succulent & healthy' 62.4%, while the use of a single descriptor had no impact on the rate of apple selection ('healthy' 50.5% and 'succulent' 52%. The strongest predictors of individual dessert choice were the taste score given to the apple, and the lack of belief that healthy foods are not tasty. Interventions that emphasize the taste attributes of healthier foods are likely to be more effective at achieving healthier diets than those emphasizing health alone.

  6. Detection of drought tolerant genes within seedling apple rootstocks in Syria

    Science.gov (United States)

    This investigation was conducted to detect the drought tolerant genes (four genes) within seedling apple rootstocks derived from five apple genotypes, including Syrian apple cultivars. The results showed that the gene MdPepPro (a cyclophilin) was found in all studied genotypes and their progenies e...

  7. Identification of selected apple pests based on selected graphical parameters

    Science.gov (United States)

    Boniecki, P.; Koszela, K.; Piekarska-Boniecka, H.; Nowakowski, K.; Przybył, J.; Zaborowicz, M.; Raba, B.; Dach, J.

    2013-07-01

    The aim of this work was a neural identification of selected apple tree orchard pests. The classification was conducted on the basis of graphical information coded in the form of selected geometric characteristics of agrofags, presented on digital images. A neural classification model is presented in this paper, optimized using learning sets acquired on the basis of information contained in digital photographs of pests. In particular, the problem of identifying 6 selected apple pests, the most commonly encountered in Polish orchards, has been addressed. In order to classify the agrofags, neural modelling methods were utilized, supported by digital analysis of image techniques.

  8. Qualities of native apple cultivar juices characteristic of central Europe

    OpenAIRE

    Rop, Otakar; Martin POSOLDA; Mlcek, Jiri; Vojtech REZNICEK; Sochor, Jiri; Adam, Vojtech; Kizek, Rene; Sumczynski, Daniela

    2012-01-01

    During the last century due to the changes in landscape management, in country people’s lives and with intensive fruit-growing the native cultivars of apple fruit trees have been gradually disappearing. The aim of the study was to compare the juices made of native apple cider cultivars. The cultivars-‘Boikovo’, ‘Jadernicka moravska’, ‘Kardinal zihany’, ‘Panenske ceske’, ‘Parmena zlata zimni’, ‘Strymka’ growing in the locality of the Litencicke Hills in the eastern part of the Czech Republic w...

  9. The position of Apple Incorporated in the World Economy

    OpenAIRE

    Marahimi, Narmin

    2011-01-01

    My Diploma Thesis is aimed to clarify the position of the Apple Incorporated in the world economy, as well as expectations and anticipations for future. I decided to write about Apple not only because it's one of the leading company in the technology Market, but because interest to its production is increasing day by day, making it highly competitive. Coming to the content of my work: It consists of 3 chapters, with several subchapters being included into each chapter. The first chapter is an...

  10. Instant Apple iBooks how-to

    CERN Document Server

    Chawdhary, Zeeshan

    2013-01-01

    Filled with practical, step-by-step instructions and clear explanations for the most important and useful tasks. Get the job done and learn as you go. Written in a step-by-step tutorial style, this book shows you in simple steps how to get the most from your iBooks experience.This book is for literary geeks who want to explore the Apple iBooks universe. From buying books from the Apple iBookstore to organizing books and PDFs, this book goes forward by explaining how to write an interactive book with iBooks Author.

  11. Ethylene Emission of Apples Treated with 1-Methylcyclopropene During Storage

    OpenAIRE

    Melnyk Oleksandr; Drozd Olga; Boicheva Natalia; Zhmudenko Yuliia; Melnyk Igor; Khudik Liudmyla; Remeniuk Leonid; Vykhvatniuk Liubomyr

    2014-01-01

    The effect of post-harvest application of 1-methylcyclopropene (1-MCP) on ethylene emission in early-winter apple (cv. ‘Calville’), winter (‘Gloster’, ‘Golden Delicious’ and ‘Jonagold’), and late-winter cultivars (‘Granny Smith’, ‘Idared’ and ‘Reinette Simirenko’) w as investigated. The fruits were picked at a commercial harvest time and half of them were treated with 1-MCP (‘SmartFresh™’ 0.068 gm-3) for 24 h at 5 °C. Then both groups of apples were placed in a common storage at the temperatu...

  12. APPLE & GOOGLE: A COMPARATIVE ANALYSIS OF MARKETING APPROACHES AND STRATEGIES

    OpenAIRE

    Ashley Wouters

    2014-01-01

    This paper focuses on the marketing strategies of Google and Apple. By comparing literature on both Apple’s and Google’s marketing approaches and strategies, we try to discover what makes these companies different from each other and/or what makes them alike. Besides a great focus on their customers when developing their products, both their key value is product leadership. The paper looks at their most important product/service introductions. For Apple this is the introduction of iPod an...

  13. [Unhealthy weight loss. Erosion by apple cider vinegar].

    Science.gov (United States)

    Gambon, D L; Brand, H S; Veerman, E C I

    2012-12-01

    Erosive tooth wear was diagnosed in the dentition of a 15-year-old girl with a Moroccan background. After an anamnesis, extensive analysis of possible risk factors and a study of the pattern of erosion, it was concluded that the erosive tooth wear was induced by daily consumption of a glass of apple cider vinegar Further investigation revealed that in North-African culture, women have used apple cider vinegar to achieve weight loss for generations. Bodybuilders are also known to make use of this method of weight reduction. PMID:23373303

  14. Nuclear magnetic resonance characterization of apple juice containing enzyme preparations

    International Nuclear Information System (INIS)

    In this work, 1H nuclear magnetic resonance (1H NMR) was employed to evaluate changes in apple juice in response to the addition of Panzym Yieldmash and Ultrazym AFP-L enzymatic complexes and compare it with premium apple juice. The juice was processed at different temperatures and concentrations of enzymatic complexes. The differences in the results were attributed mainly to the enzyme concentrations, since temperature did not cause any variation. A quantitative analysis indicated that the concentration of fructose increased while the concentrations of sucrose and glucose decreased in response to increasing concentrations of the enzymatic complexes. (author)

  15. Effect of ion implantation on apple wine yeast

    International Nuclear Information System (INIS)

    The wild type apple wine yeast Y02 was treated by ion implantation with the dose of 8 x 1015 ion/cm2. As results, a special mutant strain, IONII-11 dry, was obtained. The morphology characters, partial biochemistry characters, mycelium protein of the mutant strain were distinctively changed compared with original strain Y02. After the fermentation test ,the apple wine producing rate of the mutant strain increased 22.4% compared with original strain. These results showed that ion implantation was an effective method for mutagenesis

  16. Regional somatic embryogenesis from in vitro apple leaf and artificial seed of apple

    International Nuclear Information System (INIS)

    First three open leaves of in vitro apple (Malus domastica Borkh.) shoots were used as explants. Each leaf was divided into three regions (upper, middle and lower) from tip to base and each region was composed of two sub-regions (leaf and right) along the main vain. Every explant was pricked with a dissector at the center of one sub-region before inoculated on MS + BA 1 mg/L + NAA 4 mg/L + 2,4-D 0.5 mg/L medium and incubated in darkness for 7 days, then transferred to MS + BA 1 mg/L medium. 85% of the explants regenerated somatic embryos directly around the prick after 40 days. The embryos were encapsulated in 4% sodium alginate and 2% CaCl2 as artificial seeds, which could germinate and grow into plantlets under aseptic condition

  17. Epigenome targeting by probiotic metabolites

    Directory of Open Access Journals (Sweden)

    Licciardi Paul V

    2010-12-01

    Full Text Available Abstract Background The intestinal microbiota plays an important role in immune development and homeostasis. A disturbed microbiota during early infancy is associated with an increased risk of developing inflammatory and allergic diseases later in life. The mechanisms underlying these effects are poorly understood but are likely to involve alterations in microbial production of fermentation-derived metabolites, which have potent immune modulating properties and are required for maintenance of healthy mucosal immune responses. Probiotics are beneficial bacteria that have the capacity to alter the composition of bacterial species in the intestine that can in turn influence the production of fermentation-derived metabolites. Principal among these metabolites are the short-chain fatty acids butyrate and acetate that have potent anti-inflammatory activities important in regulating immune function at the intestinal mucosal surface. Therefore strategies aimed at restoring the microbiota profile may be effective in the prevention or treatment of allergic and inflammatory diseases. Presentation of the hypothesis Probiotic bacteria have diverse effects including altering microbiota composition, regulating epithelial cell barrier function and modulating of immune responses. The precise molecular mechanisms mediating these probiotic effects are not well understood. Short-chain fatty acids such as butyrate are a class of histone deacetylase inhibitors important in the epigenetic control of host cell responses. It is hypothesized that the biological function of probiotics may be a result of epigenetic modifications that may explain the wide range of effects observed. Studies delineating the effects of probiotics on short-chain fatty acid production and the epigenetic actions of short-chain fatty acids will assist in understanding the association between microbiota and allergic or autoimmune disorders. Testing the hypothesis We propose that treatment with

  18. Loss of Body Weight and Fat and Improved Lipid Profiles in Obese Rats Fed Apple Pomace or Apple Juice Concentrate

    OpenAIRE

    Cho, Kyung-Dong; Han, Chan-Kyu; Lee, Bog-Hieu

    2013-01-01

    The purpose of this study was to investigate the influence of apple pomace (AP) and apple juice concentrate (AC) supplementation on body weight and fat loss as well as lipid metabolism in obese rats fed a high-fat diet. Diet-induced obese rats were assigned to three groups (n=8 for each group): high fat diet (HFD) control, HFD containing 10% (w/w) AP, and HFD containing 10% (w/w) AC. There was also a normal diet group (n=8). After 5 weeks, body weight gain, adipose tissue weight, serum and he...

  19. Antifungal Activity of Microbial Secondary Metabolites

    OpenAIRE

    Jeffrey J Coleman; Ghosh, Suman; Okoli, Ikechukwu; Mylonakis, Eleftherios

    2011-01-01

    Secondary metabolites are well known for their ability to impede other microorganisms. Reanalysis of a screen of natural products using the Caenorhabditis elegans-Candida albicans infection model identified twelve microbial secondary metabolites capable of conferring an increase in survival to infected nematodes. In this screen, the two compound treatments conferring the highest survival rates were members of the epipolythiodioxopiperazine (ETP) family of fungal secondary metabolites, acetylg...

  20. Secondary metabolites in fungus-plant interactions

    OpenAIRE

    Pusztahelyi, Tünde; Holb, Imre J.; Pócsi, István (1961-) (biológus)

    2015-01-01

    Fungi and plants are rich sources of thousands of secondary metabolites. The genetically coded possibilities for secondary metabolite production, the stimuli of the production, and the special phytotoxins basically determine the microscopic fungi-host plant interactions and the pathogenic lifestyle of fungi. The review introduces plant secondary metabolites usually with antifungal effect as well as the importance of signaling molecules in induced systemic resistance and systemic acquired resi...

  1. Probabilistic Approach for Evaluating Metabolite Sample Integrity

    OpenAIRE

    Slaff, Barry M.; Jensen, Shane T.; Weljie, Aalim M.

    2015-01-01

    The success of metabolomics studies depends upon the "fitness" of each biological sample used for analysis: it is critical that metabolite levels reported for a biological sample represent an accurate snapshot of the studied organism's metabolite profile at time of sample collection. Numerous factors may compromise metabolite sample fitness, including chemical and biological factors which intervene during sample collection, handling, storage, and preparation for analysis. We propose a probabi...

  2. Field Evaluation of Traditional Apple Cultivars to Induced Diseases and Pests

    OpenAIRE

    János BÁLINT; Rezső THIESZ; NYÁRÁDI, Imre-István; Károly-Attila SZABÓ

    2013-01-01

    During the survey we studied traditional old apple cultivars in order to test disease resistance, as part of an apple breeding program. Diseases and pests assessments were made throughout the two growing season in 2008 and 2009 in traditional apple cultivars (‘Batul’, ‘Pónyik’, and ‘Sóvári’) from stray and commercial (‘Golden Delicious’ and ‘Starkrimson’) apple orchards. Our data add to a body of evidence indicating that traditional apple cultivars under natural selection and without pesticid...

  3. Metabolites from Alternaria Fungi and Their Bioactivities

    Directory of Open Access Journals (Sweden)

    Ligang Zhou

    2013-05-01

    Full Text Available Alternaria is a cosmopolitan fungal genus widely distributing in soil and organic matter. It includes saprophytic, endophytic and pathogenic species. At least 268 metabolites from Alternaria fungi have been reported in the past few decades. They mainly include nitrogen-containing metabolites, steroids, terpenoids, pyranones, quinones, and phenolics. This review aims to briefly summarize the structurally different metabolites produced by Alternaria fungi, as well as their occurrences, biological activities and functions. Some considerations related to synthesis, biosynthesis, production and applications of the metabolites from Alternaria fungi are also discussed.

  4. Apple and peach consumption habits across European countries

    NARCIS (Netherlands)

    Konopacka, D.; Jesionkowska, K.; Kruczynska, D.; Schoorl, F.W.

    2010-01-01

    The aim of this study was to gain information concerning apple and peach consumption frequency within different European countries in relation to age and gender. The survey was a part of a complex experiment with the aim of evaluating consumers’ preferences towards new varieties, and the data is bas

  5. ECONOMIC EFFICIENCY OF DIFFERENT PROTECTION TREATMENTS IN APPLE PRODUCTION

    Directory of Open Access Journals (Sweden)

    Vesna Tomaš

    2015-06-01

    Full Text Available Apple is the most represented fruit species in Croatia. Codling moth, Cydia pomonella L, is one of the most important apple pests whose population is growing from year to year. The aim of this study was to determine the economic effectiveness of four treatments against codling moth (1 - based on baculovirus; 2 - based on the group of synthetic pyrethroid; 3 - based on kaolin, 4 - control treatment, on the three apple varieties. The experiment was performed at the Agricultural Institute Osijek, Croatia, during three years (2012-2014. In order to analyze the results of apple production it was necessary to calculate production efficiency, labor productivity, and profitability of production. The results of the research of economic efficiency according to market prices treatment 1 and treatment 2 had economic coefficient above 1 with tendency of significant growth, while treatment 3 and 4 were uneconomical. The treatment 1showed advantage over the treatment 2 because of its positive effects on human health and biodiversity, as well as satisfactory economic efficiency.

  6. Characterization and degradation of pectin derived from Budimka apple

    Directory of Open Access Journals (Sweden)

    MILOS V. NIKOLIC

    2008-02-01

    Full Text Available The characterization of apple pectin and its oligogalacturonic fractions derived from the autochthones apple variety Budimka, characteristic for central Serbia, is described in this paper. After extraction, the apple pectin was subjected to controlled enzymatic hydrolysis by polygalacturonase (PG and pectin lyase (PL from Aspergillus niger and then fractionated by ion-exchange column chromatography on Dowex 1X-8 (200–400 mesh. Saturated oligogalacturonic acids, obtained by controlled hydrolysis with PG, were efficiently separated by elution with a gradient of Na acetate buffer (pH 6.0, while unsaturated oligogalacturonic acids, obtained by controlled hydrolysis with PL, were separated on the same resin, using a gradient of Na formate buffer (pH 4.7 as the eluent. The yields of the fractions with the particular degree of polymerization (DP were also determined. The total content of neutral saccharides in the original Budimka apple pectin was detected by HPLC analysis of the 4-nitrobenzoyl derivatives of the sugar, and amounted to 5.31 %. Among the neutral saccharides, contents of galactose, glucose, rhamnose, arabinose, xylose and mannose were detected.

  7. Ultrastructure study of apple meristem cells during cryopreservation

    Science.gov (United States)

    The ultrastructure of apple (Malus x domestica Borkh.) meristem cells was studied before and after cold acclimation (CA) and during the steps of PVS2 vitrification. We compared cells of in vitro grown shoots of two cultivars, Grushovka Vernenskaya and Voskhod. Cells of the two cultivars were simila...

  8. Experimental investigation on light propagation through apple tissue structures

    Science.gov (United States)

    Askoura, Mohamed Lamine; Piron, Vianney; Vaudelle, Fabrice; L'Huillier, Jean-Pierre; Madieta, Emmanuel; Mehinagic, Emira

    2015-07-01

    The interaction of light with biological materials, such as fruits and vegetables, is a complex process which involves both absorption, and scattering events at different scales. Measuring the optical properties of a fruit allows understanding the physical and chemical characteristics. In this paper, an optical bench based on the use of a continuous laser source and a CCD camera was developed to study the light diffusion inside apple tissue structures. The method refers to the well-known steady-state spatially resolved method. First, the optoelectronics system was tested with a tissue phantom in order to show the optimal sensing range required to obtain the best estimated optical properties. Second, experimental results were obtained using peeled and unpeeled apples as interrogated tissues. The data were confronted with a diffusion model in order to extract the optical properties at two wavelengths of 633, and 852 nm. To better understand the effect of the apple tissue structures, investigations into the propagation of light through a half cut apple were also performed.

  9. A Computerized Weather Station for the Apple IIe.

    Science.gov (United States)

    Lorson, Mark V.

    Predicting weather conditions is a topic of interest for students who want to make plans for outside activities. This paper discusses the development of an inexpensive computer-interfaced classroom weather station using an Apple IIe computer that provides the viewer with up to the minute digital readings of inside and outside temperature,…

  10. Seasonality of nutrients in leaves and fruits of apple trees

    Directory of Open Access Journals (Sweden)

    Nachtigall Gilmar Ribeiro

    2006-01-01

    Full Text Available The nutrient accumulation curves of apple trees are good indicators of plant nutrient demand for each developmental stage. They are also a useful tool to evaluate orchard nutritional status and to estimate the amount of soil nutrient removal. This research aimed at evaluating the seasonality of nutrients in commercial apple orchards during the agricultural years of 1999, 2000, and 2001. Therefore, apple tree leaves and fruits of three cultivars 'Gala', 'Golden Delicious' and 'Fuji' were weekly collected and evaluated for fresh and dry matter, fruit diameter and macronutrient (N, P, K, Ca and Mg and micronutrient (B, Cu, Fe, Mn, and Zn concentrations. Leaf and fruit sampling started one or two weeks after full bloom, depending on the cultivar, and ended at fruit harvest or four weeks later (in the case of leaf sampling. In general, leaf concentrations of N, P, K, Cu, and B decreased; Ca increased; and Mg, Fe, Mn, and Zn did vary significantly along the plant vegetative cycle. In fruits, the initial nutrient concentrations decreased quickly, undergoing slow and continuous decreases and then remaining almost constant until the end of fruit maturation, indicating nutrient dilution, once the total nutrient accumulation increased gradually with fruit growth. Potassium was the nutrient present in highest quantities in apple tree fruits and thus, the most removed from the soil.

  11. Ripe Fuji Apple Detection Model Analysis in Natural Tree Canopy

    Directory of Open Access Journals (Sweden)

    Dongjian He

    2012-11-01

    Full Text Available In this work we develop a novel approach for the automatic recognition of red Fuji apples within a tree canopy using three distinguishable color models in order to achieve automated harvesting. How to select the recognition model is important for the certain intelligent harvester employed to perform in real orchards. The L*a*b color model, HSI (Hue, Saturation and Intensity color model and LCD color difference model, which are insensitive to light conditions, are analyzed and applied to detect the fruit under the different lighting conditions because the fruit has the highest red color among the objects in the image. The fuzzy 2-partition entropy, which could discriminate the object and the background in grayscale images and is obtained from the histogram, is applied to the segment the Fuji apples under complex backgrounds. A series of mathematical morphological operations are used to eliminate segmental fragments after segmentation. Finally, the proposed approach is validated on apple images taken in natural tree canopies. A contribution reported in this work, is the voting scheme added to the natural tree canopy which recognizes apples under different light influences.

  12. Apple Grade Judgment Based on the Neural Network

    Institute of Scientific and Technical Information of China (English)

    BAO Xiao-an; LUO Zhuo-lin; ZHANG Rui-lin

    2004-01-01

    A processing method on the basis of the technology of computer visual and digital image was introduced. The improved LVQ (learning vector quantization) neural network algorithm applied in the process to identify the grade of apples was proved effective in experiment.

  13. POSTPROCESSING DIP MAINTAINS QUALITY OF FRESH-CUT APPLE

    Science.gov (United States)

    An aqueous solution with hypochlorite as a sanitizer; sodium erythorbate (isoascorbate), N-acetylcysteine and 4-hexylresorcinol as reducing and anti-browning agents; and Ca propionate as a firming agent was developed for postprocessing dip of fresh-cut 'Gala' apple. The additional effect of edible ...

  14. Storage test on apple juice after ultrasound treatment

    Directory of Open Access Journals (Sweden)

    Filomena Montemurro

    2014-03-01

    Full Text Available Apple juice, for its sensory and nutritional qualities, is consumed by people of all ages. Apples are an excellent source of several phenolic compounds and the presence of polyphenols is recognized for their health promoting antioxidant properties. Thermal pasteurization of fruit juices is the conventional method used for their preservation. Therefore, this constitutes the most extensively available methods for the inactivation of microorganisms in fruit juices but it causes side effects on their flavour and nutritional quality. Consumers tend to prefer recently extracted juices with fresh taste and minimal flavor or vitamin losses. To meet consumers’ demand, among the novel technologies that involve non-thermal processes, power ultrasound have been investigated as an alternative to conventional heat treatments. Objective of this study was to evaluate the effectiveness of the use of ultrasound in an attempt to maintain the organoleptic characteristics typical of a natural apple juice. In particular, it was evaluated the action on the microflora residing and shelf life of the product through microbiological and sensory analyses. Juice treated with ultrasound highlighted a reduction of aerobic mesophilic counts and psychrophilic bacteria respectively about 3 and 5 log CFU/mL and an enhanced yeast growth. The general opinion expressed by the panelist was in favour of the sonicated juice. This preliminary study showed that non-thermal methods such as power ultrasound technology may give new opportunities to develop fresh-like apple juice.

  15. Newton's Apple: 15th Season. Free Educational Materials.

    Science.gov (United States)

    Twin Cities Public Television, St. Paul, MN.

    This guide helps teachers use the 15th season of the television program "Newton's Apple" in the classroom and lists show segments on asthma, car engines, glacier climbing, glass blowing, glaucoma, gliders, gold mine, greenhouse effect, kids on Mars, lightning, "Lost World" dinosaurs, mammoth dig, NASA robots, Novocain (TM), pack behavior, pet…

  16. Apple Stack Cake for Dessert: Appalachian Regional Foods

    Science.gov (United States)

    Shortridge, Barbara G.

    2005-01-01

    How is the culture of Appalachia conveyed through its foods? Local experts in Appalachian counties were asked to create a hypothetical menu for a meal that was representative of their home region. Fried chicken and ham were the preferred main dishes and dessert selections focused on apple pie and peach or blackberry cobbler. Virtually everyone…

  17. An explorative study of the effect of apple and apple products on the human plasma metabolome investigated by LC–MS profiling

    DEFF Research Database (Denmark)

    Rago, Daniela; Gürdeniz, Gözde; Ravn-Haren, Gitte;

    2015-01-01

    pomace (22 g/day), or no supplement. Plasma was collected for analysis after an overnight fast and analysed by UPLC-ESI-TOF-MS. Discriminative features revealed by Partial Least Square-Discriminant Analysis showed whole apple and apple pomace having generally a stronger modifying effect of the plasma...... metabolome than the other apple products. We observed an effect on branched-chain amino acids and aromatic amino acids degradation, and a decreased use of lipid fuels indicating an improvement in glucose utilisation. A reduced level of plasma bile acids after apple consumption may indicate less re...

  18. Technical Efficiency and Productivity of Apple Farming in Antalya Province of Turkey

    Directory of Open Access Journals (Sweden)

    Mevlut Gul

    2005-01-01

    Full Text Available This study analyzes social-economic characteristics of apple farms in Antalya province and reveals economic structure of apple farming. Technical efficiency of apple farming was also analyzed. Data were collected through personal interview from 60 agricultural enterprises during 2001 production year. Average size of the apple orchards in the study area is 12.46 da. Share of the total apple land in total farm land is 28.47%. Annual average gross margin of apple production consists of 67.91% of total gross margin of the farms surveyed. Traditional farming technique is prevailing in the region and farmers interviewed are growing traditional apple varieties. Unit cost of apple produced on the farms surveyed was calculated to be 161.269 TL kg-1. On the other hand, apple is sold at a price of 280.581 TL kg-1. Accordingly, profit margin was calculated to be 119.312 TL kg-1. Technical efficiencies of apple production were estimated with Data Envelopment Analysis (DEA and found to be 0.69 and 0.92 for constant and variable returns to scale assumptions, respectively. The greatest input slacks were in fertilizer use (K, N and P respectively. Therefore, to achieve a technically efficient apple production, appropriate soil tests should be conducted to determine fertilizer requirements of the soils.

  19. Study on the radiation preservation of apples and the radiation effects

    International Nuclear Information System (INIS)

    The radiation effects on respiration and ethylene production in apple and its radiation preservation were reported in this paper. It shows that, when irradiation was applied immediately after harvest, the respiratory rate and ethylene production increase with the increasing of irradiation dose. When irradiation was applied after 7-10 days of storage, the respiratory rate still rises with the dose, while there is a decrease in ethylene production with the increasing of dose. If the irradiation dose is less than 800 Gy the rise of respiratory rate of irraadiated apple returns to the level of the control within 5 days, but ethylene production of irradiated apple keeps a lower level. According to the above results, dose of 300-500 Gy may be recommended for radiation preseration of apple. Ascorbic acid is unstable and sensitive to radiation. The radiation effects on ascorbic acid in apple is very small because the saccharides and some organic acid in apple, especially malic acid, are able to protect ascorbic acid from radiation damage. From measuring the saccharides and acids in apple, it is obvious that there is no significant difference between irradiated and non-irradiated apple. Long-life free radical in apple was not detected after irradiation. The scald of apple is reduced from 60% to less than 15% after cold storage for 8 monthes. The taste results indicated, tasting them without knowing which is irradiated or not, that the irradiated apple is superior to controlled sample

  20. Portable programming on parallel/networked computers using the Application Portable Parallel Library (APPL)

    Science.gov (United States)

    Quealy, Angela; Cole, Gary L.; Blech, Richard A.

    1993-01-01

    The Application Portable Parallel Library (APPL) is a subroutine-based library of communication primitives that is callable from applications written in FORTRAN or C. APPL provides a consistent programmer interface to a variety of distributed and shared-memory multiprocessor MIMD machines. The objective of APPL is to minimize the effort required to move parallel applications from one machine to another, or to a network of homogeneous machines. APPL encompasses many of the message-passing primitives that are currently available on commercial multiprocessor systems. This paper describes APPL (version 2.3.1) and its usage, reports the status of the APPL project, and indicates possible directions for the future. Several applications using APPL are discussed, as well as performance and overhead results.

  1. Nondestructive evaluation of free acid content in apples using near-infrared spectroscopy

    International Nuclear Information System (INIS)

    In non-destructive evaluation of free acid content in apples by near- infrared spectroscopy(NIRS), browning and heat treatment of squeezed apple juice affected to the accuracy but titratable alkali concentration did not. The free acid content in apples after harvest was able to determine using different apples in harvest time for calibration making. The result of MLR, multiple correlation coefficient(R) was 0.77 and standard error of prediction(SEP) was 0.03%. The free acid content in apples during storage was able to determine using calibration equation established with stored apples, R was 0.90 and SEP was ca. 0.04%. The prediction accuracy by NIR was not sufficient for use of quantitative analysis of free acid content in apple, but classification of low and high level in acid content was supposed to be applicable

  2. Physical and sensory properties of ready to eat apple chips produced by osmo-convective drying.

    Science.gov (United States)

    Velickova, Elena; Winkelhausen, Eleonora; Kuzmanova, Slobodanka

    2014-12-01

    The low cost raw material, apple variety Idared, was turned into value-added product, apple chips. The apple chips were produced in a two-step process consisting of osmotic treatment and conventional drying. Osmotic treatment was carried out in 40 % glucose solution at room temperature, followed by convective drying at 105 °C, till reaching water activity of 0.3. Mechanical properties of the apple chips measured by compression and penetration tests were correlated with the sorption properties. The low browning index, 25.5 and high whitening index, 63.7, proved the good retention of the color of the apple chips. The instrumental characteristics of the apple chips were correlated with the sensory characteristics of the product. The new product was accepted by the 95 % of the panelists. The stored apple chips under modified atmosphere showed no significant changes in the quality parameters over 6 month period. PMID:25477635

  3. Integrating reflectance and fluorescence imaging for apple disorder classification

    Science.gov (United States)

    Ariana, Diwan P.; Guyer, Daniel E.; Shrestha, Bim P.

    2004-03-01

    Multispectral imaging in reflectance and fluorescence modes combined with neural network analysis was used to classify various types of apple disorder from three apple varieties (Honey Crisp, Red Cort, and Red Delicious). Eighteen images from a combination of filter sets and three different imaging modes (reflectance, visible light induced fluorescence, and UV induced fluorescence) were acquired for each apple sample as a base for pixel-level classification into normal or disorder tissue. Two classification schemes, a 2-class and a multiple class, were developed and tested in this study. In the 2-class scheme, pixels were categorized into normal or disorder tissue, whereas in the multiple class scheme, pixels were categorized into normal, bitter pit, black rot, decay, soft scald, and superficial scald tissues. Results indicate that single variety training under the 2-class scheme yielded highest accuracy with total accuracy of 95, 97, and 100 % for Honey Crisp, Red Cort, or Red Delicious respectively. In the multiple-class scheme, the classification accuracy of Honey Crisp apple for normal, bitter pit, black rot, decay, and soft scald tissue was 94, 93, 97, 97, and 94 % respectively. Through variable selection analysis, in the 2-class scheme, fluorescence models yielded higher total classification accuracy compared to reflection models. For Red Cort and Red Delicious, models with only FUV yield more than 95% classification accuracy, demonstrating a potential of fluorescence to detect superficial scald. Several important wavelengths, including 680, 740, 905 and 940 nm, were identified from the filter combination analysis. The results indicate the potential of this technique to accurately recognize different types of disorder on apple.

  4. Phenolic Composition and Sensory Properties of Ciders Produced from Latvian Apples

    Directory of Open Access Journals (Sweden)

    Riekstina-Dolge Rita

    2014-07-01

    Full Text Available Polyphenol compounds are very important components of cider – they are responsible for the colour and the bitterness and astringency balance of cider. The polyphenolic profile of apples and apple drinks is influenced by several factors: apple variety, climate, maturity, and technological processes applied. This research paper concerns the influence of apple variety on the phenolic compounds and sensory properties of cider. Fermentation of 12 varieties of apple juice with Saccharomyces cerevisiae yeast ‘71B-1122’ (Lalvin, Canada was performed in a laboratory of the Faculty of Food Technology of the Latvia University of Agriculture. The total phenol content (TPC was determined according to the Folin-Ciocalteu spectrophotometric method. Individual phenolic compounds were analysed using HPLC. Sensory properties (clarity, the apple, fruit and yeast aroma, the apple and yeast taste, sourness, astringency, and bitterness were evaluated by trained panelists using a line scale. Special attention was paid to the use of dessert apples for the production of cider. The most important sensory properties of cider are the aroma and taste of apples and fruit. All cider samples showed the intensity of apple aroma ranging from 5.3 to 7.6 points, and higher results were obtained for cider from the bvariety ‘Auksis’ apples. The TPC in cider samples varied from 792.68 to 3399.78 mg L-1: Among crab apples, the highest TPC was detected in ciders made from the ‘Hyslop’ and ’Riku’ varieties, whereas among dessert apples, the highest TPC was detected in ciders made from the ‘Antonovka’ variety. Among the twelve phenols identified in cider samples, chlorogenic acid and caffeic acid were the dominating ones. Variation in the sensory properties of ciders was dependent on the physicochemical composition of the apples used.

  5. Intake of whole apples or clear apple juice has contrasting effects on plasma lipids in healthy volunteers

    DEFF Research Database (Denmark)

    Ravn-Haren, Gitte; Dragsted, Lars Ove; Buch-Andersen, Tine;

    2013-01-01

    : Apples are rich in polyphenols and pectin, two potentially bioactive constituents; however, these constituents segregate differently during processing into juice products and clear juice is free of pectin and other cell wall components. We conclude that the fibre component is necessary for the...

  6. Would You Like that iPhone Locked or Unlocked?: Reconciling Apple's Anticircumvention Measures with the DMCA

    OpenAIRE

    Daniel J. Corbett

    2008-01-01

    When Apple's iPhone first hit the stores it was an epochal media event.2 Apple, long a leader in high-end computers and personal electronics, was poised to make its entry into ahighly-competitive market with a new mobile phone that promised groundbreaking technological capabilities in a sleek, ergonomic package. Apple's CEO, Steve Jobs, extolled the iPhone's virtues to an eager press, and, shortly thereafter, Apple's stock jumped dramatically.3 Apple's loyal devotees lined up in anticipation ...

  7. Effects of location within the tree canopy on carbohydrates, organic acids, amino acids and phenolic compounds in the fruit peel and flesh from three apple (Malus × domestica) cultivars.

    Science.gov (United States)

    Feng, Fengjuan; Li, Mingjun; Ma, Fengwang; Cheng, Lailiang

    2014-01-01

    Fruits from three cultivars of apple (Malus × domestica Borkh.)-'McIntosh', 'Gala' and 'Mutsu'-were harvested from the exterior and interior of the tree canopy. Peel and flesh tissues were sampled separately to determine how the position of the fruit on the tree might affect the levels of the primary and secondary metabolites in the fruit. Fruit from the outer-canopy had a higher fresh weight and a higher soluble solids content compared with inner-canopy fruit. Both the flesh and peel of the outer-canopy fruit had higher concentrations of soluble sugars and sugar alcohols, but lower starch concentrations than the inner-canopy fruit. Canopy position did not significantly affect malic acid concentrations, except in the peel of 'McIntosh' and the flesh of 'Mutsu'. Although levels of ascorbic and succinic acids were higher in the peel of the outer-canopy fruit, the responses of other organic acids to canopy position depended on tissue type and cultivar. Except for histidine, lysine, threonine and glycine, most amino acids accumulated at higher concentrations in the inner-canopy fruit. By contrast, levels of phenolic compounds from both the peel and flesh were significantly higher in the outer-canopy fruit. The significant effects of location within the canopy on both primary metabolites and secondary metabolites demonstrate the importance of light exposure on apple fruit quality. PMID:26504536

  8. Secondary metabolites from marine Penicillium brevicompactum

    OpenAIRE

    2006-01-01

    In a screening of Basidiomycete cultures isolated from marine invertebrates collected along the Chilean coastline for the production of antibiotics we identified a Penicillium brevicompactum strain as a producer of metabolites inhibiting the growth of bacteria and fungi. Bioactivity guided purification resulted in the isolation of four known metabolites. Their structures were elucidated by spectroscopic methods.

  9. A New Paradigm for Known Metabolite Identification in Metabonomics/Metabolomics: Metabolite Identification Efficiency

    Directory of Open Access Journals (Sweden)

    Jeremy R. Everett

    2015-01-01

    Full Text Available A new paradigm is proposed for assessing confidence in the identification of known metabolites in metabonomics studies using NMR spectroscopy approaches. This new paradigm is based upon the analysis of the amount of metabolite identification information retrieved from NMR spectra relative to the molecular size of the metabolite. Several new indices are proposed including: metabolite identification efficiency (MIE and metabolite identification carbon efficiency (MICE, both of which can be easily calculated. These indices, together with some guidelines, can be used to provide a better indication of known metabolite identification confidence in metabonomics studies than existing methods. Since known metabolite identification in untargeted metabonomics studies is one of the key bottlenecks facing the science currently, it is hoped that these concepts based on molecular spectroscopic informatics, will find utility in the field.

  10. The Effects of Drop Height and Padding Surface on Bruising of Exportable Apple

    Directory of Open Access Journals (Sweden)

    D Ghanbarian

    2016-04-01

    Full Text Available Unfortunately despite the great ranking of Iran for apple production around the world, the export potential is not suitable. It seems that one of the major causes of poor quality for Iranian apple varieties is bruising damage of this product. Therefore, in this study, some factors affecting the apple bruising were addressed. For this purpose, factorial experiment in a completely randomized design with 72 treatments, including variety factor in three levels (Golden Delicious, Red Delicious and Granny Smith, type of padding surface in four levels (Cardboard on plastic, wood, Rubber on steel and apple and the drop height in six levels (5, 15, 25, 35, 45 and 55 cm with four replications were considered. Moreover, the maximum allowable drop heights of apples along with bruising volume estimation models were studied. Analysis of variance (ANOVA showed that bruising area and volume were significantly affected by all experimental parameters at the 1% level. The comparison test revealed that Granny Smith has tougher tissues and is less prone to vulnerability. Based on the results of this study, the maximum allowable drop heights for the Red Delicious, Golden Delicious and Granny Smith varieties were found to be 12, 15 and 20 cm, respectively. In addition, the effect of apple variety on the dependent parameters was significant. Based on the findings of this study, the bruising due to the impact of apple and apple was lower for the moving apples compared with the stationary apples.

  11. Field Evaluation of Traditional Apple Cultivars to Induced Diseases and Pests

    Directory of Open Access Journals (Sweden)

    János BÁLINT

    2013-05-01

    Full Text Available During the survey we studied traditional old apple cultivars in order to test disease resistance, as part of an apple breeding program. Diseases and pests assessments were made throughout the two growing season in 2008 and 2009 in traditional apple cultivars (‘Batul’, ‘Pónyik’, and ‘Sóvári’ from stray and commercial (‘Golden Delicious’ and ‘Starkrimson’ apple orchards. Our data add to a body of evidence indicating that traditional apple cultivars under natural selection and without pesticide regime are highly and/or moderately resistant to fire blight, apple powdery mildew and apple scab. High resistance was observed for green apple aphid (‘Sóvári’ and rosy leaf-curling aphid (all cultivars, except ‘Sóvári’ throughout the assessment. Susceptibility from mediate to high level was observed for fruit peel moth and pear-leaf blister moth, while resistance and/or moderate resistance for spotted tentiform leafminer and codling moth. The codling moth damages were significantly correlated with seed number in fruits throughout the whole vegetation period. We can conclude that old apple cultivars sustained in their original regions could be a significant source of genes for apple breeding programs.

  12. APPLE-3: improvement of APPLE for neutron and gamma-ray flux, spectrum and reaction rate plotting code, and of its code manual

    International Nuclear Information System (INIS)

    A code APPLE was produced in 1976 for calculating and plotting tritium breeding ratio and tritium production rate distributions. That code was improved as 'APPLE-2' in 1982, to calculate and plot not only tritium breeding ratio but also distributions of neutron and gamma-ray fluxes, their spectra, nuclear heating rates and other reaction rates, and dose rate distributions during operation and after shutdown in 1982. The code APPLE-2 can calculate and plot these nuclear properties derived from neutron and gamma-ray fluxes by ANISN (one dimensional transport code), DOT3.5 (two dimensional transport code) and MORSE (three dimensional Monte Carlo code). We revised the code APPLE-2 as 'APPLE-3' by adding many functions to the APPLE-2 code in accordance with users' requirements proposed in recent progress of fusion reaction nuclear design. With minor modification of APPLE-2, a number of inconsistencies have been found between the code manual and the input data in the code. In the present report, the new functions added to APPLE-2 and improved users' manual are explained. (author)

  13. Application of mass spectrometry for metabolite identification.

    Science.gov (United States)

    Ma, Shuguang; Chowdhury, Swapan K; Alton, Kevin B

    2006-06-01

    Metabolism studies play a pivotal role in drug discovery and development. Characterization of metabolic "hot-spots" as well as reactive and pharmacologically active metabolites is critical to designing new drug candidates with improved metabolic stability, toxicological profile and efficacy. Metabolite identification in the preclinical species used for safety evaluation is required in order to determine whether human metabolites have been adequately tested during non-clinical safety assessment. From an instrumental standpoint, high performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) dominates all analytical tools used for metabolite identification. The general strategies employed for metabolite identification in both drug discovery and drug development settings together with sample preparation techniques are reviewed herein. These include a discussion of the various ionization methods, mass analyzers, and tandem mass spectrometry (MS/MS) techniques that are used for structural characterization in a modern drug metabolism laboratory. Mass spectrometry-based techniques, such as stable isotope labeling, on-line H/D exchange, accurate mass measurement to enhance metabolite identification and recent improvements in data acquisition and processing for accelerating metabolite identification are also described. Rounding out this review, we offer additional thoughts about the potential of alternative and less frequently used techniques such as LC-NMR/MS, CRIMS and ICPMS. PMID:16787159

  14. Design and performance of the APPLE-Knot undulator

    Energy Technology Data Exchange (ETDEWEB)

    Ji, Fuhao [Department of Physics, State Key Laboratory of Surface Physics, and Laboratory of Advanced Materials, Fudan University, 2005 Songhu Road, Shanghai 200438, People’s Republic of (China); Chang, Rui [State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, People’s Republic of (China); Zhou, Qiaogen; Zhang, Wei [Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 239 Zhangheng Road, Shanghai 201204, People’s Republic of (China); Ye, Mao [State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, People’s Republic of (China); Sasaki, Shigemi [Hiroshima Synchrotron Radiation Center, Hiroshima University, 2-313 Kagamiyama, Hiroshima 739-0046 (Japan); Qiao, Shan, E-mail: qiaoshan@mail.sim.ac.cn [State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, People’s Republic of (China); School of Physical Science and Technology, ShanghaiTech University, 319 Yueyang Road, Shanghai 200031, People’s Republic of (China)

    2015-06-09

    The design and performance of the Apple-Knot undulator which can generate photons with arbitrary polarization and low on-axis heat load are presented. Along with the development of accelerator technology, synchrotron emittance has continuously decreased. This results in increased brightness, but also causes a heavy heat load on beamline optics. Recently, optical surfaces with 0.1 nm micro-roughness and 0.05 µrad slope error (r.m.s.) have become commercially available and surface distortions due to heat load have become a key factor in determining beamline performance, and heat load has become a serious problem at modern synchrotron radiation facilities. Here, APPLE-Knot undulators which can generate photons with arbitrary polarization, with low on-axis heat load, are reported.

  15. Epiphytic lichens of apple orchards in Poland, Slovakia, and Italy

    Directory of Open Access Journals (Sweden)

    Daria Zarabska

    2013-12-01

    Full Text Available Following the study of epiphytic lichens in 30 apple orchards from Poland, Slovakia and Italy the list of 74 taxa was prepared. The most common are the meso- to xerophytic and heliophilous species. The highest number of taxa was observed in Slovak orchards. Moreover, lichens shared with at least one other country were also noted mainly in Slovakia. Bark of apple trees seems to create favourable habitats for Bacidia rubella, which together with Strangospora pinicola were valuable founds in Polish orchards. In Slovak orchards, special attention should be paid to Acrocordia gemmata, Melanelixia glabra and Usnea hirta. Among interesting records in Italian orchards, Phaeophyscia hispidula and Ph. kairamoi can be mentioned.

  16. Quality of apples following gamma irradiation and cold storage

    International Nuclear Information System (INIS)

    The physico-chemical and organoleptic quality changes in apple cvs ‘Golden Delicious’, ‘Royal Delicious’, ‘Red Delicious’ and ‘Rich-A-Red’ exposed to gamma radiation doses of 0.1, 0.2, 0.4 and 0.6 kGy for quarantine and preservation purposes, were studied up to 6 months of storage at 2–4°C. Among the four cvs, ‘Rich-A-Red’ treated with 0.1 kGy dose showed better retention of sensory attributes and minimal changes in texture, total soluble solids, acidity and vitamin C content during storage. Results indicate that irradiation has commercial potential for apples as an alternative quarantine treatment for export requirements

  17. SPECIES COMPOSITION OF WEED VEGETATION IN DIFFERENT APPLE GROWING TECHNOLOGIES

    Directory of Open Access Journals (Sweden)

    Venera TASSEVA

    2005-08-01

    Full Text Available The investigation was carried out in the period 2001-2003 in an orchard of the Institute of Agriculture, Kyustendil, Bulgaria, created in the spring of 1996 on leached cinnamonic forest soil. The weed populations under four different farming technologies of growing of apple cultivar Florina were investigated. It was established, that the apple growing technologies influence the weed association composition. The highest weed diversity was found in the organic technology - 16 weed species were found. In the application of resource economical and integrated technologies, the development of 13-14 weed species was established. The smallest weed diversity was observed in the conventional technology - eight species, which was due to the twofold herbicide application.

  18. A new antioxidant beverage produced with green tea and apple.

    Science.gov (United States)

    Rubio-Perez, Jose M; Vidal-Guevara, Maria L; Zafrilla, Pilar; Morillas-Ruiz, Juana M

    2014-08-01

    Green tea and apple are natural products with health benefits. These healthy properties are linked closely to the antioxidant compounds, mainly phenolic compounds. These antioxidant compounds have a potential for preventing and treating cancer, cardiovascular, inflammatory and neurodegenerative diseases in humans. The aim of the present work was to design a new beverage with high antioxidant power combining extracts of green tea and apple, studying the antioxidant composition and activity, organoleptic properties (colour) and stability status during storage at different temperatures. The majority compounds identified in the beverage were flavan-3-ols, being the (-)-epigallocatechin-3-gallate which had the highest concentration. After storage, floridzine was the compound with lower decrease of concentration. The new designed beverage had a good colour, and high antioxidant activity and stability at room temperature, so that the beverage needs no refrigeration, showing potential for the development of new healthy functional beverages. PMID:24601928

  19. Study on biologically active substances in irradiated apple juice

    International Nuclear Information System (INIS)

    The radiochemical changes proceeding by irradiation of foodstuffs rich in carbohydrates are studied. For the purpose pure solutions of D-glucose, D-fructose and sucrose and fresh apple juice, irradiated with 0,5 and 1,0 Mrad are investigated. Changes set in UV-spectra of the irradiated foodstuffs, the specific reaction of malonic dialdehyde formation with 2-thiobarbituric acid and the formation of carbonyl compounds reacting with 2,4-dinitro phenylhydrazine are studied. Results show that in the irradiated sample solutions of sugars and apple juice two peaks are formed. The malonic dialdehyde formation depends on the dose of irradiation applied. The newly formed carbonyl compounds both in the sample solutions and in the juice are 8 to 9 in number. (author)

  20. A study of the physiological changes and the nutritional qualities of irradiated apples and the effect of irradiation on apples stored at room temperature

    International Nuclear Information System (INIS)

    The effects of γ-irradiation on the metabolism and nutritional qualities of Golden Delicious apples and on the lethality of pathogenic fungi have been studied. The storage effect of irradiation on apples at room temperature has been observed. Results showed that the respiratory intensity of irradiated apple at 0.3-0.5 kGy was near or lower than that of unirradiated apple after 15 days irradiation. The amount of ethylene release was obviously inhibited when fruits were irradiated with 0.3-0.7 kGy. The flesh firmness of apples irradiated with 0.3-0.9 kGy was higher than that of unirradiated apple with increased storage time. The negative correlations between the flesh firmness and the activities of pectinesterase (PE), polygalacturonase (PG) were observed when the dosage was lower than 1.5 kGy. The 2.0 kGy irradiation damaged the ultrastructure of cells, induced the softening of apple. When apples were irradiated with 0.7-2.0 kGy, the contents of 4 important volatile components of apple would be decreased. However, this dose had no effects on the pure chemicals. Studies showed that there was no significant effect of irradiation with 0.3-2.0 kGy on the nutritional qualities of apples and this dose range could effectively control the verticillate pathogenic fungi. The result of storage experiment showed that rotting of fruits decreased by 0.3-0.9 kGy irradiation. (author)

  1. Slow softening of Kanzi apples (Malus×domestica L.) is associated with preservation of pectin integrity in middle lamella.

    Science.gov (United States)

    Gwanpua, Sunny George; Verlinden, Bert E; Hertog, Maarten L A T M; Nicolai, Bart M; Hendrickx, Marc; Geeraerd, Annemie

    2016-11-15

    Kanzi is a recently developed apple cultivar that has an extremely low ethylene production, and maintains its crispiness during ripening. To identify key determinants of the slow softening behaviour of Kanzi apples, a comparative analysis of pectin biochemistry and tissue fracture pattern during different ripening stages of Kanzi apples was performed against Golden Delicious, a rapid softening cultivar. While substantial pectin depolymerisation and solubilisation was observed during softening in Golden Delicious apples, no depolymerisation or increased solubilisation was observed in Kanzi apples. Moreover, tissue failure during ripening was mainly by cell breakage in Kanzi apples and, in contrast, by cell separation in Golden Delicious apples. Kanzi apples had lower activity of beta-galactosidase, with no decline in the extent of branching of the pectin chain. A sudden decrease in firmness observed during senescence in Kanzi apples was not due to middle lamella dissolution, as tissue failure still occurred by cell breakage. PMID:27283709

  2. Effects of location within the tree canopy on carbohydrates, organic acids, amino acids and phenolic compounds in the fruit peel and flesh from three apple (Malus × domestica) cultivars

    OpenAIRE

    Fengjuan Feng; Mingjun Li; Fengwang Ma; Lailiang Cheng

    2014-01-01

    Fruits from three cultivars of apple (Malus × domestica Borkh.)—‘McIntosh’, ‘Gala’ and ‘Mutsu’—were harvested from the exterior and interior of the tree canopy. Peel and flesh tissues were sampled separately to determine how the position of the fruit on the tree might affect the levels of the primary and secondary metabolites in the fruit. Fruit from the outer-canopy had a higher fresh weight and a higher soluble solids content compared with inner-canopy fruit. Both the flesh and peel of the ...

  3. A Tour of the Goldstone-Apple Valley Radio Telescope

    Science.gov (United States)

    Ardenski, Brooke; Stephan, George R.

    1997-01-01

    Goldstone-Apple Valley Radio Telescope (GAVRT) is located in a remote area of the Mojave Desert, 40 miles north of Barstow, California. The antenna, identified as DSS-12, is a 34-meter diameter dish, 11 times the diameter of a ten foot microwave dish used for satellite television. DSS-12 has been used by NASA to communicate with robotic space probes for more than thirty years.

  4. Apple can act as anti-aging on yeast cells.

    OpenAIRE

    Vanessa Palermo; Fulvio Mattivi; Romano Silvestri; Giuseppe La Regina; Claudio Falcone; Cristina Mazzoni

    2012-01-01

    International audience In recent years, epidemiological and biochemical studies have shown that eating apples is associated with reduction of occurrence of cancer, degenerative, and cardiovascular diseases. This association is often attributed to the presence of antioxidants such as ascorbic acid (vitamin C) and polyphenols. The substances that hinder the presence of free radicals are also able to protect cells from aging. In our laboratory we used yeast, a unicellular eukaryotic organism,...

  5. Beyond-root calcium fertilization of apple trees

    Directory of Open Access Journals (Sweden)

    Kazimierz Słowik

    2013-12-01

    Full Text Available Investigations were performed in the period 1977-1979 on the apple tree cultivar 'Fantazja', on rootstock A 2, M 7 and MM 106 on the effect of spraying with solution containing calcium on the incidence of bitter pit, breakdown, calcium content in the fruit flesh and other features of the fruits. Threefold spraying with calcium nitrate, calcium chloride or Anti-Stipp significantly limited the appearance of bitter pit and breakdown.

  6. Chemopreventive effect of apple and berry fruits against colon cancer

    OpenAIRE

    Jaganathan, Saravana Kumar; Vellayappan, Muthu Vignesh; Narasimhan, Gayathri; Supriyanto, Eko; Octorina Dewi, Dyah Ekashanti; Narayanan, Aqilah Leela T; Balaji, Arunpandian; Subramanian, Aruna Priyadarshini; Yusof, Mustafa

    2014-01-01

    Colon cancer arises due to the conversion of precancerous polyps (benign) found in the inner lining of the colon. Prevention is better than cure, and this is very true with respect to colon cancer. Various epidemiologic studies have linked colorectal cancer with food intake. Apple and berry juices are widely consumed among various ethnicities because of their nutritious values. In this review article, chemopreventive effects of these fruit juices against colon cancer are discussed. Studies de...

  7. It was easy, when apples and blackberries were only fruits

    OpenAIRE

    Yerva, Surender Reddy; Miklós, Zoltán; Aberer, Karl

    2010-01-01

    Ambiguities in company names are omnipresent. This is not accidental, companies deliberately chose ambiguous brand names, as part of their marketing and branding strategy. This procedure leads to new challenges, when it comes to finding information about the company on the Web. This paper is concerned with the task of classifying Twitter messages, whether they are related to a given company: for example, we classify a set of twitter messages containing a keyword apple, whether a message is re...

  8. Apple II software for M13 shotgun DNA sequencing.

    OpenAIRE

    Larson, R; Messing, J

    1982-01-01

    A set of programs is presented for the reconstruction of a DNA sequence from data generated by the M13 shotgun sequencing technique. Once the sequence has been established and stored other programs are used for its analysis. The programs have been written for the Apple II microcomputer. A minimum investment is required for the hardware and the software is easily interchangeable between the growing number of interested researchers. Copies are available in ready to use form.

  9. Determinants of Sustainable Relationships in the Albanian Apple Production Sector

    OpenAIRE

    Blendi Gërdoçi; Engjell Skreli; Drini Imami

    2016-01-01

    This paper analyses the behaviour of Albanian apple growers and their relations with buyers based on a structured survey. We develop a model of relational governance that highlights the importance of sustainable (lasting) relationships and draws upon different theoretical frameworks such as transaction cost economics and social network theory and focuses on determinants of relational exchange. The findings support the social network argument that the presence of verbal agreements between busi...

  10. Dinamiche del mercato televisivo e il caso Apple Tv

    OpenAIRE

    Gambaro Marco; Pilotti Luciano

    2014-01-01

    The history of Apple is quite clear in its strategies and trajectoriesabout competition in the global techno-media towards a radical evolution of multimedia devices as an eco-system with the transformation of a user's evolved, educated and socialized by the global community for sharing data, information and knowledge encapsulated in a proprietary model (almost) closed. The main focus is a large connecting trajectory of technology and content, with a global community of users. A story of succe...

  11. Sooty blotch of apple: Efficacy of different application strategies

    OpenAIRE

    Mayr, U.; Späth, S.

    2008-01-01

    Sooty blotch causes heavy losses in Lake Constance organic apple production. In the last five years research has been done at the research station for fruit growing (Kompetenzzentrum Obstbau – Bodensee) on strategies to control Sooty blotch on organic grown pome fruit. Different control strategies with lime sulphur, coconut soap and potassium bicarbonate with different application rates, application times were tested in several trials on the cultivar ‘Topaz’. Over the years the...

  12. Physico-chemical evaluation of radiation effects on apple juice

    International Nuclear Information System (INIS)

    Gala and Fuji varieties apple's juice were clarified with enzyme and irradiated aiming to extend the shelf-life without conservants and chemical additives. The juices were analysed for soluble solids, titrable acidity, pH and color. Results showed effect of storage periods in soluble solids, pH and color. The variety and storage period modified the titrable acidity. The pH was altered by irradiation dose and the storage period. (author). 9 refs., 6 figs

  13. Apple's iPad City: subcontracting exploitation to China

    OpenAIRE

    J. Chan; Pun, N; Selden, M; Kees, VDP; van der Pijl, Kees

    2015-01-01

    Many image-conscious technology companies, probably none more than Apple in our digital age, have professed ideals of corporate citizenship, environmental, labour and social responsibility in their supplier codes of conduct. This is in part a response to the growing anti-sweatshop movement in the electronics industry from within the United States, Europe, and more recently Greater China (Smith et al. 2006; Litzinger 2013). Violations of factory workers’ fundamental rights in export-oriented i...

  14. TV≠电视机——APPLE TV

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    苹果越来越不像是一家电脑公司了:层出不穷的iPod们俨然已经成了MP3的代名词,iTunes网上音乐商店生意兴隆,不久前推出的iPhone在手机市场上掀起了轩然大波,而今,苹果又带来了Apple TV。

  15. A wood preservative metabolite in river water.

    Science.gov (United States)

    Khoroshko, Larisa O; Petrova, Varvara N; Viktorovskii, Igor V; Lahtiperä, Mirja; Sinkkonen, Seija; Paasivirta, Jaakko

    2005-01-01

    A previously unknown pollutant in river water was identified to be 2-mercaptobenzothiazole (2-MBT) by interpretation and simulation of its GC/LRMS spectrum. Further GC/HRMS measurement of the isotope composition of the molecular ion verified this structure. 2-MBT is a well-known agent for corrosion inhibition and a stable metabolite of several other benzothiazoles. The present 2-MBT trace was most probably a metabolite of the wood preservative TCMTB which leaked from an upstream sawmill. The metabolite had been detected earlier in urine of the sawmill workers, but now was identified in the recipient water environment for the first time. PMID:15768735

  16. Vegetative Propagation of Five Local Cultivars of Malay Apple (Syzygium malaccense spp. in Ternate Island

    Directory of Open Access Journals (Sweden)

    A.R. Ryadin

    2014-01-01

    Full Text Available The characteristics of cuttings of five cultivars of local malay apple (Syzygium malaccense L. was studied in Ternate Island, Indonesia. Hardwood cuttings of malay apple were planted in two combinations of media and hormone treatments. During 5 months experiment, can be found that hardwood cutting of malay apple with top soil+ sand and NAA 1000 ppm had the best performance in producing shoots and roots for cuttings of malay apple in a short period. Although, each of these cultivars has different performance to rooting and shooting, but its can be propagated by cuttings. Therefore, the result of this research is expected to contribute for conservation of malay apple as a source of germplasm for further research and commercialization of malay apple as new potential fruit in the international market demand.

  17. Distribution of multiple pesticide residues in apple segments after home processing

    DEFF Research Database (Denmark)

    Rasmussen, Rie Romme; Poulsen, Mette Erecius; Hansen, H. C. B.

    2003-01-01

    The effects of washing, storing, boiling, peeling, coring and juicing on pesticide residue were investigated for field-sprayed Discovery and Jonagold apples. Residues of chlorpyrifos, cypermethrin, deltamethrin, diazinon, endosulfan, endosulfan sulfate, fenitrothion, fenpropathrin, iprodione......, kresoxim-methyl, lambda-cyhalothrin, quinalphos, tolylfluanid and vinclozolin in the processed apples were analysed by gas chromatography. Statistical analysis showed that reductions of 18-38% were required to obtain significant effects of processing practices, depending on pesticide and apple variety....... Juicing and peeling the apples significantly reduced all pesticide residues. In the case of detectable pesticide residues, 1-24% were distributed in the juice and in the peeled apple. None of the pesticide residues was significantly reduced when the apples were subject to simple washing or coring. Storing...

  18. Secondary Metabolites from Rubiaceae Species

    Directory of Open Access Journals (Sweden)

    Daiane Martins

    2015-07-01

    Full Text Available This study describes some characteristics of the Rubiaceae family pertaining to the occurrence and distribution of secondary metabolites in the main genera of this family. It reports the review of phytochemical studies addressing all species of Rubiaceae, published between 1990 and 2014. Iridoids, anthraquinones, triterpenes, indole alkaloids as well as other varying alkaloid subclasses, have shown to be the most common. These compounds have been mostly isolated from the genera Uncaria, Psychotria, Hedyotis, Ophiorrhiza and Morinda. The occurrence and distribution of iridoids, alkaloids and anthraquinones point out their chemotaxonomic correlation among tribes and subfamilies. From an evolutionary point of view, Rubioideae is the most ancient subfamily, followed by Ixoroideae and finally Cinchonoideae. The chemical biosynthetic pathway, which is not so specific in Rubioideae, can explain this and large amounts of both iridoids and indole alkaloids are produced. In Ixoroideae, the most active biosysthetic pathway is the one that produces iridoids; while in Cinchonoideae, it produces indole alkaloids together with other alkaloids. The chemical biosynthetic pathway now supports this botanical conclusion.

  19. Secondary metabolites from Rubiaceae species.

    Science.gov (United States)

    Martins, Daiane; Nunez, Cecilia Veronica

    2015-01-01

    This study describes some characteristics of the Rubiaceae family pertaining to the occurrence and distribution of secondary metabolites in the main genera of this family. It reports the review of phytochemical studies addressing all species of Rubiaceae, published between 1990 and 2014. Iridoids, anthraquinones, triterpenes, indole alkaloids as well as other varying alkaloid subclasses, have shown to be the most common. These compounds have been mostly isolated from the genera Uncaria, Psychotria, Hedyotis, Ophiorrhiza and Morinda. The occurrence and distribution of iridoids, alkaloids and anthraquinones point out their chemotaxonomic correlation among tribes and subfamilies. From an evolutionary point of view, Rubioideae is the most ancient subfamily, followed by Ixoroideae and finally Cinchonoideae. The chemical biosynthetic pathway, which is not so specific in Rubioideae, can explain this and large amounts of both iridoids and indole alkaloids are produced. In Ixoroideae, the most active biosysthetic pathway is the one that produces iridoids; while in Cinchonoideae, it produces indole alkaloids together with other alkaloids. The chemical biosynthetic pathway now supports this botanical conclusion. PMID:26205062

  20. PRELIMINARY CHARACTERIZATION OF ROMANIAN APPLE CULTIVARS AFTER COLD STORAGE

    Directory of Open Access Journals (Sweden)

    Butac Mădălina

    2011-01-01

    Full Text Available Eight Romanian apple (Malus domestica Borkh. cultivars (Rebra, Rustic, Bistriţean, Auriu de Bistriţa, Goldprim, Ciprian, Irisem, Redix have been studied for their post-harvest behavior and consumer acceptance in 2008. The main objectives of this study have been the characterization of some physical-chemical parameters (weight, size, firmness, and total soluble solids that influence fruit quality, before and after cold storage. Apples were submitted to normal air storage during 3 months, at 20C and 80% relative air humidity. Fruit weight varied between 182.5 g (Auriu de Bistriţa cv. and 122 g (Rustic cv.. Maximum diameter has been registered on the Auriu de Bistriţa and Rebra cultivars (85 and 80 mm, respectively, while Rustic fruit were the thinnest (65 mm. The highest level of refractometric index (18.2º Brix has been recorded in Redix fruit, moderate (16.4º Brix in Ciprian fruit and the lowest soluble solids (13.4º Brix has been acquired in apples from Rustic cultivar. Pulp firmness has been higher (5.9 kgf/cm2 in Redix fruit than in all the other cultivars under study. Ciprian cultivar seems to be of the high quality showing an attractive external appearance and promising storage behavior.

  1. Magnetic design for Knot-APPLE undulator and its performance

    International Nuclear Information System (INIS)

    In order to generate a low energy photon beam in a high energy storage ring with an insertion device, it is necessary to increase the undulator's deflection parameter, K. If this is the case, a high heat load on beamline elements is a serious problem because the on-axis radiation power increases drastically as increasing K parameter for a linear undulator. To overcome this problem, a Figure-8, a Pera, a Knot undulator were proposed. However, these undulators cannot change polarizations. On the other hand, APPLE undulator is capable for generating variable polarization. A novel Knot-APPLE undulator was proposed. It is capable for reducing on-axis high heat load and generating every polarization state. But we have some difficulties for displacing radiation power peak from higher harmonics off-axis due to differences in gap dependence between horizontal field and vertical one especially in vertical linear mode for a present magnet structure. In this paper, we looked for most appropriate magnet size and structure to obtain ideal magnetic field in every polarization state. In the presentation, we show radiation spectra and corresponding power density distributions expected from another possible magnet structure for Knot-APPLE undulator. (author)

  2. Modified atmosphere efficiency in the quality maintenance of Eva apples

    Directory of Open Access Journals (Sweden)

    Camila Argenta Fante

    2014-06-01

    Full Text Available Modified atmosphere is a method of food preservation that provides increased lifetime, decreases deterioration losses, and facilitates marketing. The objective of this study was to evaluate the efficiency of different plastic films in modifying the atmosphere around Eva apples to assure quality maintenance during postharvest storage. The fruits were cleaned and separated into three treatment groups: polypropylene, low density polyethylene, and high density polyethylene packing with a total of 5 fruits per package for each evaluation period. A group of control apples was not submitted to atmospheric modification. After the treatment, all fruits were stored at 0.5±0.5°C (cold storage for up to 225 days. The analyses were performed at 45, 135, and 225 days after cold storage. Respiration, ethylene production, firmness, mass loss, total pectin, soluble pectin, soluble solids, total acidity, and epidermis background color of each treatment group were evaluated. The high density polyethylene film treatment did not show a decrease in ethylene production during storage and allowed the fruits to maintain a greater firmness and smaller percentage of mass loss during the study period. Moreover, the storage of the Eva apple cultivar under modified atmosphere allowed the preservation of quality for up to seven months.

  3. Development of complete hydrolysis of pectins from apple pomace.

    Science.gov (United States)

    Wikiera, Agnieszka; Mika, Magdalena; Starzyńska-Janiszewska, Anna; Stodolak, Bożena

    2015-04-01

    Enzymatically extracted pectins have a more complex structure than those obtained by conventional methods. As a result, they are less susceptible to hydrolysis, which makes the precise determination of their composition difficult. The aim of the study was to develop a method of complete hydrolysis of enzymatically extracted apple pectins. Substrates were pectins isolated from apple pomace by the use of xylanase and multicatalytic preparation Celluclast and apple pomace. Hydrolysis was performed by a chemical method with 2M TFA at 100 °C and 120 °C and a combined acidic/enzymatic method. After hydrolysis, the contents of galacturonic acid and neutral sugars were measured by HPLC. Complete hydrolysis of polygalacturonic acid occurred after 2.5h incubation with 2M TFA at 120 °C. The efficient hydrolysis of neutral sugars in pectins was performed with 2M TFA at 100 °C for 2.5h. Monomers most susceptible to concentrated acid were rhamnose, mannose and arabinose. PMID:25442606

  4. DEVELOPMENT OF MICROFLORA IN APPLE ROOT RHIZOSPERE WHEN FERTILIZING

    Directory of Open Access Journals (Sweden)

    Kuzin A. I.

    2015-09-01

    Full Text Available The problem of environmental safety of agricultural products and agro-technical measures is very urgent now. Various agrochemicals are widely used in agricultural production: plant protection preparations, fertilizers, stimulants and plant growth regulators, etc. One possible solution of this problem may be a partial replacement of mineral fertilizers by bacterial ones. The aim of our research was to determine the effect of different fertilizers and methods of their application on the quantity of microorganisms in the apple root rhizosphere soil. The research was carried out according to conventional methods. In our experiment, we determined the number of microorganisms in the rhizosphere of the roots of apple trees, nitrogen, phosphorus and potassium in the leaves; hydrolysable nitrogen, available phosphorus and exchangeable potassium in soil and crop yield. The highest yield was obtained by fertigation in our research. Application bacterial fertilizer to the soil ensured yield increase at the level of mineral fertilizer application. Application of fertilizers in general, in the studied application rates provided optimum level of nitrogen, phosphorus and potassium content in the leaves and soil. By use of fertigation and bacterial fertilizers were high and relatively stable numbers of microorganisms in the rhizosphere of the apple trees. The number of microorganisms in the rhizosphere had a positive correlation with yield and the content of nutrients in soil and in leaves

  5. Effects of water extracts of apple and pomegranate peel on the quality of orange squash

    OpenAIRE

    Salah-ud Din; Kashif Akram; Umar Farooq; Afshan Shafi; Farkhandah Sarfraz; Hafeez ur Rehman; Muhammad Asim Ijaz Sidhu

    2015-01-01

    Punica granatum (pomegranate) and Malus domestica (apple) are rich source of bioactive compounds i.e. phenolics components, anthocyanins and tannins that can be potential preservative agents. The aim of the present research plan was to evaluate the quality characteristics of orange squash after addition of aqueous extracts of apple and pomegranate peels. For this purpose aqueous extracts of pomegranate and apple peels were added in orange squash and then the final product was examined for phy...

  6. Pesticide residues in individual versus composite samples of apples after fine or coarse spray quality application

    DEFF Research Database (Denmark)

    Poulsen, Mette E.; Wenneker, Marcel; Withagen, Jacques;

    2012-01-01

    versus coarse spray quality application on residue levels. The applications included boscalid, bupirimate, captan, fenoxycarb, indoxacarb, pirimicarb, pyraclostrobin and thiophanate-methyl. Apples were collected from four zones in the tree and pesticide residues were detected in the individual apples...... fine and coarse spray quality, except for carbendazim, the degradation product of thiophanate-methyl, where fine spray quality resulted in higher carbendazim residue levels than coarse spray quality. To examine the relationship between individual results and average results from ten apples, 20...

  7. Control of sooty blotch and black rot of apple through removal of fruit mummies

    OpenAIRE

    Beer Margarita; Brockamp Leona; Weber Roland W.S.

    2015-01-01

    Several popular apple cultivars retain their aborted fruits as mummies on the tree. In laboratory conditions, overwintered fruit mummies collected from a Northern German apple orchard under organic management released inoculum, which caused black rot due to Diplodia seriata and sooty blotch due to Peltaster cerophilus on ripe apples. In a field trial conducted over four years in another organic orchard, the manual removal of fruit mummies in winter and again in late June of each year signific...

  8. Changes in Phenolic Composition of Apple Juices by HPLC with Direct Injection.

    OpenAIRE

    Suárez, Belén; Picinelli, A.M. (Anna); Moreno, Javier; Mangas, J.J. (Juan)

    2011-01-01

    An analytical procedure for the quantitative determination of the major polyphenols in apple juices which successfully employs a RP-HPLC system without prior treatment of the sample is described. Apple juices were clarified by microfiltration and ultrafiltration without previous treatment. Polyphenols monitored were influenced by clarification technology employed for stabilising the apple juices. The p-coumaric acid derivative was significantly affected by membrane type and filtration time, w...

  9. Fluid intake and industrial processing in apple juice induced chronic non-specific diarrhoea.

    OpenAIRE

    Hoekstra, J H; van den Aker, J H; Ghoos, Y F; Hartemink, R.; Kneepkens, C M

    1995-01-01

    Dietary factors have been shown to contribute to the occurrence or persistence of chronic non-specific diarrhoea (CNSD). Among these are low dietary fat, high fluid consumption, and the consumption of apple juice. Prompted by the clinical impression that freshly pressed and unprocessed ('cloudy') apple juice was less likely to induce diarrhoea than normal, enzymatically processed ('clear') apple juice, both juices were compared in terms of carbohydrate malabsorption, gastric emptying, and eff...

  10. The Inventory of Phytoseiid Mite on Apple Orchards in Durrës, Albania

    OpenAIRE

    AURELA SUPARAKU; ARIS HUQI; NATASHA HAKA (DURAJ)

    2014-01-01

    A survey to determine the presence and abundance of phytoseiid mite on apple orchards has been conducted in Durres (Shena-Vlash), Albania. Leaf samples were taken from five apple varieties: Pink Lady, Golden, Starking, Fuji, Gala and the phytoseiid were then extracted. As the result of the survey, two species belonging to the Phytoseiidae family were identified: Amblyseius andersoni (Chant) and Typhlodromus pyri (Scheuten). Amblyseius andersoni was found in all apple varieties analyzed in thi...

  11. Determination of Major Viral and Sub Viral Pathogens Incidence in Apple Orchards in Himachal Pradesh

    OpenAIRE

    Kumar, Surender; Singh, Rahul Mohan; Ram, Raja; Badyal, J.; Hallan, Vipin; Zaidi, A. A.; Varma, Anupam

    2011-01-01

    Apple is the major commercial horticulture crop in Himachal Pradesh and other hill states of Jammu & Kashmir, Uttarakhand and some parts of Northeastern states of India. In order to gather data on health status and incidence of virus and virus-like pathogens in apple orchards, survey was conducted in the month of June and September, 2010 in Hatkoti, Rohru, Kuthara, Jubbal and Khadapathar areas of major apple producing Shimla district of Himachal Pradesh. A total of 250 samples were collected ...

  12. Influence of cultivar, storage time, and processing on the phenol content of cloudy apple juice

    OpenAIRE

    Begić-Akagić, Asima; Spaho, Nermina; Oručević, Sanja; Drkenda, Pakeza; Kurtović, M.; Gaši, F.; Kopjar, Mirela; Piližota, Vlasta

    2011-01-01

    The aim of this work was to investigate the influence of cultivar and storage time on the total phenol content (TPC) of three actual apple cultivars (Topaz, Pinova, Pink Lady) and three autochthonous apple cultivars (Ruzmarinka, Ljepocvjetka, Paradija) as well to determine their physical and chemical characteristics. Total phenol content has been determined by Folin-Ciocalteu reagent during cold storage at 1 °C, for 60 days. Changes of apple juice samples phenolics in relation to oxidation ti...

  13. APPLE-2: an improved version of APPLE code for plotting neutron and gamma ray spectra and reaction rates

    International Nuclear Information System (INIS)

    A computer code APPLE-2 which plots the spatial distribution of energy spectra of multi-group neutron and/or gamma ray fluxes, and reaction rates has been developed. This code is an improved version of the previously developed APPLE code and has the following features: (1) It plots energy spectra of neutron and/or gamma ray fluxes calculated by ANISN, DOT and MORSE. (2) It calculates and plots the spatial distribution of neutron and gamma ray fluxes and various types of reaction rates such as nuclear heating rates, operational dose rates, displacement damage rates. (3) Input data specification is greatly simplified by the use of standard, response libraries and by close coupling with radiation transport calculation codes. (4) Plotting outputs are given in camera ready form. (author)

  14. Ammonia activates pacC and patulin accumulation in an acidic environment during apple colonization by Penicillium expansum.

    Science.gov (United States)

    Barad, Shiri; Espeso, Eduardo A; Sherman, Amir; Prusky, Dov

    2016-06-01

    Penicillium expansum, the causal agent of blue mould rot, causes severe post-harvest fruit maceration simultaneously with the secretion of d-gluconic acid (GLA) and the mycotoxin patulin in colonized tissue. The factor(s) inducing patulin biosynthesis during colonization of the host acidic environment is unclear. During the colonization of apple fruit in vivo and growth in culture, P. expansum secretes pH-modulating GLA and ammonia. Although patulin and its possible opportunistic precursor GLA accumulate together during fungal development, ammonia is detected on the colonized tissue's leading edge and after extended culture, close to patulin accumulation. Here, we demonstrate ammonia-induced transcript activation of the global pH modulator PacC and patulin accumulation in the presence of GLA by: (i) direct exogenous treatment of P. expansum growing on solid medium; (ii) direct exogenous treatment on colonized apple tissue; (iii) growth under self-ammonia production conditions with limited carbon; and (iv) analysis of the transcriptional response to ammonia of the patulin biosynthesis cluster. Ammonia induced patulin accumulation concurrently with the transcript activation of pacC and patulin biosynthesis cluster genes, indicating the regulatory effect of ammonia on pacC transcript expression under acidic conditions. Electrophoretic mobility shift assays using P. expansum PacC and antibodies to the different cleaved proteins showed that PacC is not protected against proteolytic signalling at pH 4.5 relative to pH 7.0, but NH4 addition did not further enhance its proteolytic cleavage. Ammonia enhanced the activation of palF transcript in the Pal pathway under acidic conditions. Ammonia accumulation in the host environment by the pathogen under acidic pH may be a regulatory cue for pacC activation, towards the accumulation of secondary metabolites, such as patulin. PMID:26420024

  15. Phytophthora Root and Crown Rot on Apples in Bulgaria

    Directory of Open Access Journals (Sweden)

    Mariana Nakova

    2010-01-01

    Full Text Available Phytophthora is a genus of Oomycota responsible for some of the most serious diseases with great economic impact (Judelson and Blanco, 2005. While 54 species were found in the 20th century (Erwin and Ribeiro, 1996 another 51-54 new species have been identified(Brasier, 2008 since the year 2000. They are spread worldwide and have broad range of host plants – fruit trees, citrus, forest and park species. Phytophthora can cause serious damages in orchards and nurseries of apples, cherries, etc. In Bulgaria they have been found first on young apples and cherries (1998-1999 in Plovdiv region (Nakova, 2003. Surveys have been done for discovering disease symptoms in Plovdiv and Kjustendil regions. Isolates have been obtained from infected plant material (roots and stem bases applying baiting bioassay (green apples, variety Granny Smith and/or PARP 10 selective media. Phytophthora strains were identified based on standard morphology methods – types of colonies on PDA, CMA, V 8, type and size of sporangia, oogonia and antheridia, andoospores. Cardial temperatures for their growth were tested on CMA and PDA.For molecular studies, DNA was extracted from mycelium using the DNA extraction kit.DNA was amplified using universal primers ITS 6 and ITS 4. Amplification products concentrations were estimated by comparison with the standard DNA. Sequencing was done at the Scottish Crop Research Institute (SCRI, Dundee, Scotland. Phytophthora root and crown rot symptoms first appear in early spring. Infected trees show bud break delay, have small chlorotic leaves, and branches die all of a sudden. Later symptoms are found in August-September. Leaves of the infected trees show reddish discoloration and drop down. Both symptoms are connected with lesions (wet, necrotic in appearance at stem bases of the trees.Disease spread was 2-3% in most gardens, only in an apple orchard in Bjaga (Plovdiv region it was up to 8-10%. Morphologically, the isolates acquired from

  16. Cost-benefit trade-offs of bird activity in apple orchards

    Science.gov (United States)

    Saunders, Manu E.; Luck, Gary W.

    2016-01-01

    Birds active in apple orchards in south–eastern Australia can contribute positively (e.g., control crop pests) or negatively (e.g., crop damage) to crop yields. Our study is the first to identify net outcomes of these activities, using six apple orchards, varying in management intensity, in south–eastern Australia as a study system. We also conducted a predation experiment using real and artificial codling moth (Cydia pomonella) larvae (a major pest in apple crops). We found that: (1) excluding birds from branches of apple trees resulted in an average of 12.8% more apples damaged by insects; (2) bird damage to apples was low (1.9% of apples); and (3) when trading off the potential benefits (biological control) with costs (bird damage to apples), birds provided an overall net benefit to orchard growers. We found that predation of real codling moth larvae was higher than for plasticine larvae, suggesting that plasticine prey models are not useful for inferring actual predation levels. Our study shows how complex ecological interactions between birds and invertebrates affect crop yield in apples, and provides practical strategies for improving the sustainability of orchard systems. PMID:27413639

  17. NEW VARIETIES AND THE RETURNS TO COMMODITY PROMOTION: THE CASE OF FUJI APPLES

    OpenAIRE

    Richards, Timothy J.; Patterson, Paul M.

    2000-01-01

    The Fuji apple variety is relatively new in the U.S. As a new product, questions concern the relative impact of consumer learning by experience, by variety-specific promotion, or by generic apple promotion. A two-stage (LES/LAIDS) model incorporating both types of promotion is used to estimate the effect of generic and variety specific promotion, as well as consumer experience, on the demand for Fuji apples. Estimates show each to have a positive impact, and also show new or speciality apple ...

  18. NEW VARIETIES AND THE RETURNS TO COMMODITY PROMOTION: WASHINGTON FUJI APPLES

    OpenAIRE

    Richards, Timothy J.; Patterson, Paul M.

    1998-01-01

    The Fuji apple variety is relatively new in the U.S. As a new product, questions concern the relative impact of consumer learning by experience, by variety-specific promotion, or by generic apple promotion. A two-stage (LES/LAIDS) model incorporating both types of promotion is used to estimate the effect of generic and variety specific promotion, as well as consumer experience, on the demand for Fuji apples. Estimates show each to have a positive impact, and also show new or specialty apple v...

  19. Neutron activation analysis for study of distribution patterns of organo-halogen pollutants in apple

    International Nuclear Information System (INIS)

    The distribution characteristics of organo-halogens in apple and their sources were studied by neutron activation analysis combined with statistical analysis. The results indicated that concentrations of organo-halogens in apple were in the order of organo-chlorine >> organo-bromine > organo-iodine, and concentrations of the organo-chlorine in four parts of apple were in the order of seed >> peel >> endocarp ≥ pulp. Also, the organo-chlorine, -bromine and-iodine in apple were found to have different sources. The latter two were mainly from naturally synthetic products by plant itself, while the former was mainly from anthropogenic pollutants. (authors)

  20. Learn AppleScript The Comprehensive Guide to Scripting and Automation on MAC OS X

    CERN Document Server

    Rosenthal, Hanaan

    2009-01-01

    AppleScript is an English-like, easy-to-understand scripting language built into every Mac. AppleScript can automate hundreds of AppleScriptable applications, performing tasks both large and small, complex and simple. Learn AppleScript: The Comprehensive Guide to Scripting and Automation on Mac OS X, Third Edition has been completely updated for Mac OS X Snow Leopard. It's all here, with an emphasis on practical information that will help you solve any automation problem-from the most mundane repetitive tasks to highly integrated workflows of complex systems. * Friendly enough for beginners, d

  1. Apples inflytande på grafiska användargränssnitt

    OpenAIRE

    Ahlbom, Carl

    2011-01-01

    Syftet med detta arbete är att undersöka och presentera hur företaget Apple Inc. påverkat grafiska användargränssnitt. I detta arbete har det visat sig att Apple har haft – och har ett mycket stort inflytande på grafiska användargränssnitt. I början spreds Apples grafiska användargränssnitt till datorer och senare till internet och bl.a. musikspelare samt olika mobila apparater. Apple har haft och har fortfarande, en konsekvent formgivning i dess användargränssnitt och produkter överlag, vi...

  2. The studies on apple preservation by 60Co-γ irradiation

    International Nuclear Information System (INIS)

    Studies on 60Co-γ irradiation of Golden Delicious apple have been carried out. The results showed that the optimum irradiation dosage for stored apple was ranged from 0.3-0.5 kGy. With this dosage, the contents of vitamin C and titratable acidity in these apples had no significant change compared with unirradiated apples. The respiratory rate and the amount of ethylene release were decreased after irradiation. The mortality of the verticillate pathogenic fungi was 97% at the dose of 0.5 kGy

  3. Colour change of apple as a result of storage, shelf-life, and bruising

    OpenAIRE

    Rybczyński R.; Dobrzański B.

    2002-01-01

    Two apple cultivars (Champion and Jonagold) were used to determine the colour of fruit skin in display conditions. The apples were stored for five months and then kept at shelflife conditions for an additional 15 days. The colour of each apple was measured at six points around the fruit from blush to ground colour. The measurements were performed with the Braive 6016 colorimeter according to the L*a*b* system. After storage, the colour of the apples was found to be stable, however, some chan...

  4. Effect of apple pectin on gut microbiota - qPCR in applied microbiology

    DEFF Research Database (Denmark)

    Bergström, Anders; Wilcks, Andrea; Poulsen, Morten;

    This study was part of the large European project ISAFRUIT aiming to reveal the biological explanations for the epidemiologically well-established health effects of fruits. The objective was to identify effects of apple and apple product consumption on the composition of the cecal microbial......), while no effects of apple juice, purée or pomace on microbial composition in cecum were observed. Administration of pectin derived from apples resulted in considerable changes of these DGGE profiles. A 2-fold increase in the activity of beta-glucuronidase was observed in animals fed with pectin (7% in...

  5. The Influence of Different Apple Based Supplements on the Intestinal Microbiota of Humans

    DEFF Research Database (Denmark)

    Bergström, Anders; Wilcks, Andrea; Ravn-Haren, Gitte;

    2010-01-01

    Background and objective: The present project is part of the large ISAFRUIT project, where one of the objectives is to identify effects of apple and apple product on parameters related to gut health. In a previous rat study we observed changes in the intestinal microbiota of rats fed whole apples......-free), 3) cloudy juice (apple juice with pulp), and 4) pomace (press cake from the cloudy juice production process). Fecal samples were taken before and after each diet period. After DNA extraction, Denaturing Gradient Gel Electrophoresis (DGGE) with universal primers and specific primers for...

  6. Metabolite profiles of common Stemphylium species

    DEFF Research Database (Denmark)

    Andersen, Birgitte; Solfrizzo, Michelle; Visconti, Angelo

    1995-01-01

    identified by their chromatographic and spectroscopic data (Rf values, reflectance spectrum, retention index and ultraviolet spectrum). These metabolites have been used for the chemotaxonomical characterization of Stemphylium botryosum, S. herbarum, S. alfalfae, S. majusculum, S. sarciniforme, S. vesicarium...

  7. Secondary metabolites of slime molds (myxomycetes)

    Czech Academy of Sciences Publication Activity Database

    Dembitsky, V. M.; Řezanka, Tomáš; Spížek, Jaroslav; Hanuš, L. O.

    2005-01-01

    Roč. 66, - (2005), s. 747-769. ISSN 0031-9422 Institutional research plan: CEZ:AV0Z50200510 Keywords : slime molds * myxomycetes * metabolites Subject RIV: EE - Microbiology, Virology Impact factor: 2.780, year: 2005

  8. Effects of Apple Juice Concentrate, Blackcurrant Concentrate and Pectin Levels on Selected Qualities of Apple-Blackcurrant Fruit Leather

    Directory of Open Access Journals (Sweden)

    Lemuel M. Diamante

    2013-09-01

    Full Text Available A study was conducted to determine the effects of different levels of apple juice concentrate (AJC, blackcurrant concentrate (BCC and pectin on the moisture content, water activity, color, texture and ascorbic acid content of apple-blackcurrant fruit leather using the response surface methodology. The results showed the moisture content increased with increasing pectin level and with greater increases at higher AJC and BCC levels while the water activity increased with increasing pectin level and with increasing AJC level, at low pectin levels, but with decreasing AJC, at high pectin levels. The chroma decreased with increasing pectin level and with lower values at the middle AJC level. The puncturing force decreased with increasing AJC level but with a lower value at the middle pectin level. Lastly, the ascorbic acid content increased with increasing BCC level regardless of AJC and pectin levels. There is a need to reduce the drying temperature or time of apple-blackcurrant fruit leather just enough to bring the water activity closer to 0.60, thereby increasing the moisture content resulting in higher product yield.

  9. Metabolism and metabolites of polychlorinated biphenyls (PCBs)

    OpenAIRE

    Grimm, FA; Hu, D.; Kania-Korwel, I.; Lehmler, HJ; Ludewig, G; Hornbuckle, KC; Duffel, MW; Bergman, A; Robertson, LW

    2015-01-01

    The metabolism of polychlorinated biphenyls (PCBs) is complex and has an impact on toxicity and thereby assessment of PCB risks. A large number of reactive and stable metabolites are formed in the processes of biotransformation in biota in general and in humans in particular. The aim of this document is to provide an overview of PCB metabolism and to identify metabolites of concern and their occurrence. Emphasis is given to mammalian metabolism of PCBs and their hydroxyl, methylsulfonyl, and ...

  10. Biologically active secondary metabolites from marine cyanobacteria

    OpenAIRE

    Nunnery, Joshawna K.; Mevers, Emily; Gerwick, William H

    2010-01-01

    Marine cyanobacteria are a rich source of complex bioactive secondary metabolites which derive from mixed biosynthetic pathways. Recently, several marine cyanobacterial natural products have garnered much attention due to their intriguing structures and exciting anti-proliferative or cancer cell toxic activities. Several other recently discovered secondary metabolites exhibit insightful neurotoxic activities whereas others are showing pronounced anti-inflammatory activity. A number of anti-in...

  11. Low-molecular-weight metabolite systems chemistry

    OpenAIRE

    Hadacek, Franz; Bachmann, Gert

    2015-01-01

    Low-molecular-weight metabolites (LMWMs) comprise primary or central and a plethora of intermediary or secondary metabolites, all of which are characterized by a molecular weight below 900 Dalton. The latter are especially prominent in sessile higher organisms, such as plants, corals, sponges and fungi, but are produced by all types of microbial organisms too. Common to all of these carbon molecules are oxygen, nitrogen and, to a lesser extent, sulfur, as heteroatoms. The latter can contribut...

  12. Analytical strategies for identifying drug metabolites.

    Science.gov (United States)

    Prakash, Chandra; Shaffer, Christopher L; Nedderman, Angus

    2007-01-01

    With the dramatic increase in the number of new chemical entities (NCEs) arising from combinatorial chemistry and modern high-throughput bioassays, novel bioanalytical techniques are required for the rapid determination of the metabolic stability and metabolites of these NCEs. Knowledge of the metabolic site(s) of the NCEs in early drug discovery is essential for selecting compounds with favorable pharmacokinetic credentials and aiding medicinal chemists in modifying metabolic "soft spots". In development, elucidation of biotransformation pathways of a drug candidate by identifying its circulatory and excretory metabolites is vitally important to understand its physiological effects. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) have played an invaluable role in the structural characterization and quantification of drug metabolites. Indeed, liquid chromatography (LC) coupled with atmospheric pressure ionization (API) MS has now become the most powerful tool for the rapid detection, structure elucidation, and quantification of drug-derived material within various biological fluids. Often, however, MS alone is insufficient to identify the exact position of oxidation, to differentiate isomers, or to provide the precise structure of unusual and/or unstable metabolites. In addition, an excess of endogenous material in biological samples often suppress the ionization of drug-related material complicating metabolite identification by MS. In these cases, multiple analytical and wet chemistry techniques, such as LC-NMR, enzymatic hydrolysis, chemical derivatization, and hydrogen/deuterium-exchange (H/D-exchange) combined with MS are used to characterize the novel and isomeric metabolites of drug candidates. This review describes sample preparation and introduction strategies to minimize ion suppression by biological matrices for metabolite identification studies, the application of various LC-tandem MS (LC-MS/MS) techniques for the rapid quantification and

  13. Synthesis of the major metabolites of Tolvaptan

    Institute of Scientific and Technical Information of China (English)

    Wei Li Wan; Jian Bo Wu; Fan Lei; Xiao Long Li; Li Hai; Yong Wu

    2012-01-01

    Tolvaptan is a nonpeptide arginine vasopressin (AVP) V2-receptor antagonist and used in the treatment of heart failure,cirrhosis,syndrome of inappropriate antidiuretic hormone secretion or other high-volume capacity of hyponatremia.The metabolites of tolvaptan are mainly produced by CYP3A4,including two major compounds named DM-4103 and DM-4107.Herein,the chemical synthesis of those two metabolites is described in this article for further study.

  14. Influence of aeration in the fermentative activity of Kloeckera apiculata during fermentation of apple juice

    International Nuclear Information System (INIS)

    The influence of aeration on the fermentative activity of Kloeckera apiculata RIVE 9-2-1 was studied in order to evaluate the production of metabolites of the fermentation. To achieve this, the strain was cultured in Erlenmeyer flasks containing sterilized and aroma removed apple juice, and the chemical compounds produced during fermentation in shaken (200 min-1) and static (without agitation) cultivation were determined. The results showed that the agitation of the culture medium increases production of higher alcohols (till 591.0 mg/L) compared to static cultivation, whereas on the contrary, the production of acetic acid, ethyl acetate and glycerol (260.0 ± 11.0 mg/L, 196.0 ± 10.0 mg/L y 2.6±0.2 g/L) were higher compared to shaken cultivation (222.0 ± 8.0 mg/L, 96.0 ± 4.5 mg/L and 1.8 ± 0.2 g/L) respectively. Batch cultivations carried out in bioreactor with air flux of 25 l/h reported a growth rate μ of 0.17 h-1, production of ethanol (12.5 ± 2.0 g/L) and other compounds typically produced during alcoholic fermentation. The concentration of dissolved oxygen in the fermentation medium affects its metabolism thus; insufficient amounts of oxygen would provoke a respirofermentative metabolism. The best results in terms of organoleptic quality of the fermented beverage regarding to aroma, taste and flavor was obtained when fermented in static cultivation. The control of aeration during fermentation can be used to control the synthesis of chemical compounds of sensory impact in the production of fermented beverages.

  15. Influence of processing on the heavy metal content in fruit and vegetable products. I. Lead contamination of apples residual: lead in clear apple juice

    Energy Technology Data Exchange (ETDEWEB)

    Bielig, H.J.; Bolstorff, S.; Treptow, H.

    1972-09-01

    In 24 samples of apples of different variety and origin the lead content was determined. An average value of 0.266 ppm lead was found showing a variation of 0.05 to 0.61 ppm. Lead was more concentrated in peel than in pulp. It could be removed partly by rinsing with water, which can be important for apple juice production. During apple juice production a significant amount of lead goes with the pomace depending on the yield of juice: a smaller lead content in turbid juice is due to diminished pressing. Furthermore, the lead content is reduced by enzymatic clarification and fining.

  16. Climate change and apple farming in Indian Himalayas: a study of local perceptions and responses.

    Science.gov (United States)

    Basannagari, Basavaraj; Kala, Chandra Prakash

    2013-01-01

    Apple farming is an important activity and profession of farmer communities in the Himalayan states of India. At present, the traditional apple farming is under stress due to changes in climate. The present study was undertaken in an Indian Himalayan state, Himachal Pradesh, with the major aim of studying perceptions of farmers on the effects of climate change on apple farming along the altitudinal gradient. Through questionnaire survey, the perceptions of farmers were recorded at low hills (3000 m). At all elevation range the majority of farmers reported that there was increase in atmospheric temperature, and hence at low hills 72% farmers believed that this increase in temperature was responsible for decline in fruit size and so that the quality. Thirty five percent farmers at high hills and 30% at mid hills perceived frost as a major cause for damaging apple farming whereas at low hills 24% farmers perceived hailstorm as the major deterrent for apple farming. The majority of farmers, along the altitude (92% at high hills, 79% at mid hills and 83% at low hills), reported decrease in snowfall. The majority of farmers at low altitude and mid altitude reported decline in apple farming whereas 71% farmers at high hill areas refused decline in apple farming. About 73-83% farmers admitted delay in apple's harvesting period. At mid hills apple scab and at low hills pest attack on apple crops are considered as the indicators of climate change. The change in land use practices was attributed to climate change and in many areas the land under apple farming was replaced for production of coarse grains, seasonal vegetables and other horticulture species. Scientific investigation claiming changes in Indian Himalayan climate corroborates perceptions of farmers, as examined during the present study. PMID:24205051

  17. Thinning of Apple Fruits with Foliar Fertilizers Goëmar BM 86 E and Goëmar Folical

    OpenAIRE

    Milutin Misimović; Dragana Vukojević; Nada Zavišić; Jasmina Simić

    2014-01-01

    Thinning is a regular pomotechnical measure, which is performed by using different chemicals that cause fruit drop in intensive apple orchards. It is common that some apple cultivars overbear, giving small fruits of poor quality. Effects of chemical thinners of apple fruits are as follows: higher productiveness in the next vegetation period, higher percentage of first class apples and the lesser fruit drop before harvest and so on. Hormonal thinners that are currently being used in th...

  18. Transcriptional regulation of auxin metabolism and ethylene biosynthesis activation during apple (Malus × domestica) fruit maturation

    Science.gov (United States)

    Variation exists among apple genotypes in fruit maturation and ripening patterns that influences at13 harvest fruit firmness and postharvest storability. Based on the results from our previous large-scale 14 transcriptome profiling on apple fruit maturation and well-documented auxin-ethylene crossta...

  19. Effect of apple pectin on gut microbiota - qPCR in applied microbiology

    DEFF Research Database (Denmark)

    Bergström, Anders; Wilcks, Andrea; Poulsen, Morten;

    This study was part of the large European project ISAFRUIT aiming to reveal the biological explanations for the epidemiologically well-established health effects of fruits. The objective was to identify effects of apple and apple product consumption on the composition of the cecal microbial commu...

  20. Structured-illumination reflectance imaging (SIRI) for enhanced detection of fresh bruises in apples

    Science.gov (United States)

    A structured-illumination reflectance imaging technique was developed for the detection of fresh bruises in apples. Experiments were first conducted on a strongly scattering nylon sample embedded with foreign objects of different sizes at different depths, and then on apples of two different cultiva...

  1. Spectral Absorption and Scattering Properties of Normal and Bruised Apple Tissue

    Science.gov (United States)

    Knowledge of the spectral absorption and scattering properties of apple tissue, especially bruised tissue, can help us develop an effective method for detecting bruises during postharvest sorting and grading. This research was intended to determine the optical properties of normal and bruised apple ...

  2. 7 CFR 319.56-22 - Apples and pears from certain countries in Europe.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Apples and pears from certain countries in Europe. 319.56-22 Section 319.56-22 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL... Vegetables § 319.56-22 Apples and pears from certain countries in Europe. (a) Importations allowed....

  3. Control of speck rot in apple fruit caused by Phacidiopycnis washingtonensis with pre- and postharvest fungicides

    Science.gov (United States)

    Speck rot caused by Phacidiopycnis washingtonensis is a recently reported postharvest fruit rot disease of apple. Infection of apple fruit by the fungus occurs in the orchard, but symptoms develop during storage. In this study, selected pre- and postharvest fungicides were evaluated for control of s...

  4. Preharvest applications of fungicides for control of Sphaeropsis rot in stored apples

    Science.gov (United States)

    Sphaeropsis rot caused by Sphaeropsis pyriputrescens is a recently reported postharvest fruit rot disease of apple in Washington State and causes significant economic losses. Infection of apple fruit by the fungus occurs in the orchard, but decay symptoms develop during storage or in the market. The...

  5. Characterisation of Alternaria species-groups associated with core rot of apples in South Africa

    DEFF Research Database (Denmark)

    Serdani, M.; Kang, J.C.; Andersen, Birgitte; Crous, P.W.

    2002-01-01

    Alternaria core rot of red apple cultivars is a serious post-harvest disease in South Africa. Thirty isolates of Alternaria spp. previously isolated from apple, together with reference isolates of A. alternata and A. infectoria, were characterised and grouped according to their sporulation patterns...

  6. Managing quarantine-significant post harvest diseases in Pacific Northwest apple orchards

    Science.gov (United States)

    Phacidiopycnis washingtonensis and Sphaeropsis pyriputrescens are two recently reported quarantined pathogens that cause speck rot and sphaeropsis rot, respectively, in apple. Due to quarantine regulation, export of apple from Washington State to China was banned from 2012 through 2014. Previous st...

  7. Pre and post harvest interventions for preventing potential contamination of apples with human pathogens

    Science.gov (United States)

    The possible presence of pathogens on the surface and/or inaccessible sites (calyx, stem, and/or core) of apples has implications for the microbiological safety of supplies to the fresh and fresh-cut industry. Contamination of apples with human pathogen can occur during growth, harvesting, distribut...

  8. Letter from Hong Kong: A Report on Chinese Food, Fake Apples, and IBM's Asian Strategy.

    Science.gov (United States)

    Immel, A. Richard

    1984-01-01

    Notes that microcomputer use in Hong Kong's small business community does not reflect the growth of its high-tech electronics industry and discusses IBM's influence in Hong Kong and Asia, the counterfeiting of Apple microcomputers and software, and why Apple currently has no recourse. (MBR)

  9. Sensory evaluation of character impact components in an apple model mixture

    NARCIS (Netherlands)

    Bult, J.H.F.; Schifferstein, H.N.J.; Roozen, J.P.; Boronat, E.D.; Voragen, A.G.J.; Kroeze, J.H.A.

    2002-01-01

    Food aromas generally are complex mixtures of volatiles. In the present study, we investigated the joint effects of hexyl acetate, trans-2-hexenal and 1-hexanol on the multi-attribute perception of an apple aroma. The first two substances were identified earlier as positive contributors to the apple

  10. Efficacy of supercritical carbon dioxide for nonthermal inactivation of Escherichia coli K12 in apple cider

    Science.gov (United States)

    This study evaluated the efficacy of a supercritical carbon dioxide (SCCO2) system with a gas-liquid porous metal contactor for eliminating Escherichia coli K12 in apple cider. Pasteurized, preservative-free apple cider was inoculated with E. coli K12 and processed using the SCCO2 system at CO2 conc...

  11. 75 FR 76754 - Non-Frozen Apple Juice Concentrate From China

    Science.gov (United States)

    2010-12-09

    ... review * * *'' (75 FR 69628). Accordingly, pursuant to section 751(c) of the Tariff Act of 1930 (19 U.S.C... COMMISSION Non-Frozen Apple Juice Concentrate From China AGENCY: United States International Trade Commission... 2010 to determine whether revocation of the antidumping duty order on non- frozen apple...

  12. Molecular species identification of cryptic apple and snowberry maggots (Diptera: Tephritidae) in Western and Central Washington

    Science.gov (United States)

    In Washington state, identification of the quarantine apple pest Rhagoletis pomonella (Walsh) is complicated by the presence of the cryptic species R. zephyria Snow (Diptera: Tephritidae). Distinguishing the two flies is important because there is a zero tolerance policy for R. pomonella in apple p...

  13. Irradiation disinfestation of apple maggot (Diptera: Tephritidae) in hypoxic and low-temperature storage.

    Science.gov (United States)

    Hallman, Guy J

    2004-08-01

    Apple maggot, Rhagoletis pomonella (Walsh), is a quarantine pest of apples, Malus domestica Borkhausen, and pears, Pyrus communis L., shipped from much of the United States and Canada. As such, these fruits shipped from infested areas to uninfested areas must undergo a quarantine disinfestation treatment. The objective of this research was to develop irradiation quarantine treatments against apple maggot considering that fruit hosts may be stored under hypoxic or cold conditions when they are irradiated. Hypoxia increased from 30.5 to 35.7 Gy (17%) the estimated dose to achieve 99% prevention of the full pupal stage from irradiated third instars in apples compared with ambient atmospheres. However, 50 Gy completely prevented the full pupa in 22,360 and 15,530 third instars, respectively, irradiated in apples in ambient and hypoxic atmospheres. There was no difference in development to the full pupal stage in apple maggot third instars held at 1 or 24 degrees C when irradiated with 20 Gy. Because the maximum dose measured when 50 Gy was sought was 57 Gy, the latter should be the dose recommended for quarantine disinfestation of host fruits of the apple maggot. Apples and pears tolerate much higher doses. PMID:15384333

  14. Apple Classrooms of Tomorrow: Philosophy and Structure [and] What's Happening Where.

    Science.gov (United States)

    Apple Computer, Inc., Cupertino, CA.

    Apple Classrooms of Tomorrow (ACOT) is a long-term research project sponsored by Apple Computer, Inc., to explore how learning and teaching change when teachers and students have access to interactive computer technologies. ACOT adheres to a philosophy that instruction should be learner controlled; i.e., students take responsibility for their own…

  15. Ultrasound effects on the degradation kinetics, structure and rheological properties of apple pectin.

    Science.gov (United States)

    Zhang, Lifen; Ye, Xinqian; Ding, Tian; Sun, Xiaoyang; Xu, Yuting; Liu, Donghong

    2013-01-01

    The effects of ultrasound on the molecular weight of apple pectin were investigated. The structure and rheological properties of the degradation products were also tentatively identified by High Performance Liquid Chromatography-Photodiode Array Detector (HPLC-PAD), Infrared spectroscopy (IR), Nuclear Magnetic Resonance spectroscopy (NMR) and Rheometer. The results indicated that the weight-average molecular weight of apple pectin decreased obviously after ultrasound treatment. The molecular weight of degradation products had a uniform and narrow distribution. Ultrasound intensity and temperature play an important role in the degradation reaction. Degradation kinetics model of apple pectin fitted to 1/M(t) - 1/M(0) = kt from 5 to 45 °C. The degree of methylation of apple pectin reduced according to IR analysis when ultrasound was applied. Ultrasound treatment could not alter the primary structure of apple pectin according to the results determined by HPLC, IR and NMR. Meanwhile, the viscosity of apple pectin was 10(3) times as large as that of ultrasound-treated apple pectin. The ultrasound-treated apple pectin showed predominantly viscous responses (G' pectin. PMID:22982008

  16. How to Choose Criteria to Harvest Apples. The Dynamics of Maturity

    NARCIS (Netherlands)

    Tijskens, L.M.M.; Herold, B.; Zude, M.; Geyer, M.

    2008-01-01

    The storage behaviour of apples is simulated with a model based on the changes in green ground colour of three apple cultivars (`Elstar¿, `Topaz¿ and `Pinova¿) during ripening on the tree in the same orchard in three consecutive seasons, and also the effects of at-harvest maturity criteria. Taking t

  17. Printing Appleworks V1.3 Spreadsheet Files Using the Apple IIe Computer. First Edition.

    Science.gov (United States)

    Schlenker, Richard M.; Schlenker, Deborah S.

    This guide was developed as a "how to" training device for printing spreadsheet files using AppleWorks on the Apple IIe computer with a Duodisk or two disk drives. Step-by-step instructions are provided for selecting the print option; printing entire files; and for printing specific rows, columns, or blocks of the file. For each step, a diagram of…

  18. Printing Appleworks V1.3 Word Processor Files Using the Apple IIe Computer. First Edition.

    Science.gov (United States)

    Schlenker, Richard M.; Schlenker, Deborah S.

    This guide was developed as a "how to" training device for printing word processor files using AppleWorks on the Apple IIe computer with a Duodisk or two disk drives. Step-by-step instructions are provided for file loading, selecting the print option, and printing files. For each procedure, a diagram of the screen display is included. (GL)

  19. First report of Elsinoe leaf and fruit spot and Elsinoe pyri on apple in Denmark

    DEFF Research Database (Denmark)

    Glazowska, Sylwia Emilia; Schiller, Michaela; Lund, Ole Søgaard;

    2013-01-01

    An apple disease, known as “Topaz spot” in northern Europe (Trapman and Jansonius, 2008) has since year 2000 become widespread in Danish organic apple orchards (Malus domestica). Characteristic symptoms are small spots (black on fruits, brown on leaves) having a silvery-grey cen- tre. The associa...

  20. 7 CFR 319.56-20 - Apples and pears from Australia (including Tasmania) and New Zealand.

    Science.gov (United States)

    2010-01-01

    ...) and New Zealand. 319.56-20 Section 319.56-20 Agriculture Regulations of the Department of Agriculture... Fruits and Vegetables § 319.56-20 Apples and pears from Australia (including Tasmania) and New Zealand. Apples and pears from Australia (including Tasmania) and New Zealand may be imported only in...

  1. Carbon sequestration by fruit trees--Chinese apple orchards as an example.

    Directory of Open Access Journals (Sweden)

    Ting Wu

    Full Text Available Apple production systems are an important component in the Chinese agricultural sector with 1.99 million ha plantation. The orchards in China could play an important role in the carbon (C cycle of terrestrial ecosystems and contribute to C sequestration. The carbon sequestration capability in apple orchards was analyzed through identifying a set of potential assessment factors and their weighting factors determined by a field model study and literature. The dynamics of the net C sink in apple orchards in China was estimated based on the apple orchard inventory data from 1990s and the capability analysis. The field study showed that the trees reached the peak of C sequestration capability when they were 18 years old, and then the capability began to decline with age. Carbon emission derived from management practices would not be compensated through C storage in apple trees before reaching the mature stage. The net C sink in apple orchards in China ranged from 14 to 32 Tg C, and C storage in biomass from 230 to 475 Tg C between 1990 and 2010. The estimated net C sequestration in Chinese apple orchards from 1990 to 2010 was equal to 4.5% of the total net C sink in the terrestrial ecosystems in China. Therefore, apple production systems can be potentially considered as C sinks excluding the energy associated with fruit production in addition to provide fruits.

  2. 78 FR 11173 - Kasugamycin; Receipt of Application for Emergency Exemption for Use on Apples in Michigan...

    Science.gov (United States)

    2013-02-15

    ... kasugamycin (CAS No. 6980-18-3) to treat up to 10,000 acres of apples to control fire blight. The applicant... kasugamycin on apples to control fire blight. Information in accordance with 40 CFR part 166 was submitted as... control streptomycin-resistant strains of Erwinia amylovora, the causal pathogen of fire blight, due...

  3. 77 FR 791 - Kasugamycin; Receipt of Application for Emergency Exemption for Use on Apples in Michigan...

    Science.gov (United States)

    2012-01-06

    ... Kasugamycin (CAS No. 6980-18-3) to treat up to 10,000 acres of apples to control fire blight. The applicant... use of kasugamycin on apples to control fire blight. Information in accordance with 40 CFR part 166... is needed to control streptomycin-resistant strains of Erwinia amylovora, the causal pathogen of...

  4. Improvement of elderly annual ring measure equipment with an APPLE II micro computer

    OpenAIRE

    1984-01-01

    English: With the APPLE II micro computer elderly ADDO annual ring measure desks have been improved, at a low cost, to a modern, reliable and easy operated system. Also, the APPLE itself is a stand-alone computer with many possibilities.

  5. First report of Sphaeropsis rot of apple caused by Sphaeropsis pyriputrescens in New York.

    Science.gov (United States)

    In March 2012, decayed ‘Empire’ apple fruit (Malus × domestica Borkh.) were sampled from apples stored in bins for 6 months under controlled atmosphere at a commercial packinghouse in Orleans County in New York State. The fruit were completely rotten, spongy to firm, and light brown. The incidence o...

  6. A simple multispectral imaging algorithm for detection of defects on red delicious apples

    Science.gov (United States)

    Purpose: A multispectral algorithm for detection and differentiation of defect and normal Red Delicious apples was developed from analysis of a series of hyperspectral line-scan images. Methods: A fast line-scan hyperspectral imaging system mounted on a conventional apple sorting machine was used t...

  7. How Much Cranberry Juice Is in Cranberry-Apple Juice? A General Chemistry Spectrophotometric Experiment

    Science.gov (United States)

    Edionwe, Etinosa; Villarreal, John R.; Smith, K. Christopher

    2011-01-01

    A laboratory experiment that spectrophotometrically determines the percent of cranberry juice in cranberry-apple juice is described. The experiment involves recording an absorption spectrum of cranberry juice to determine the wavelength of maximum absorption, generating a calibration curve, and measuring the absorbance of cranberry-apple juice.…

  8. 75 FR 60817 - Non-Frozen Concentrated Apple Juice From China

    Science.gov (United States)

    2010-10-01

    ... antidumping duty order on imports of non-frozen concentrated apple juice from China (65 FR 35606). Following... continuation of the antidumping duty order on imports of non- frozen concentrated apple juice from China (70 FR...), and part 207, subparts A, D, E, and F (19 CFR part 207), as most recently amended at 74 FR...

  9. ALGORITHMS FOR PARAMETERIZATION OF DYNAMICS OF INERTIA-BASED APPLE ORIENTATION

    Science.gov (United States)

    Development of machine vision systems to inspect fruit for quality and contamination problems has been stalled due to lack of a commercially-viable method for appropriately orienting fruit for imaging. We recently discovered that apples could be oriented based-on inertial properties. Apples were rol...

  10. Determinants of Sustainable Relationships in the Albanian Apple Production Sector

    Directory of Open Access Journals (Sweden)

    Blendi Gërdoçi

    2016-02-01

    Full Text Available This paper analyses the behaviour of Albanian apple growers and their relations with buyers based on a structured survey. We develop a model of relational governance that highlights the importance of sustainable (lasting relationships and draws upon different theoretical frameworks such as transaction cost economics and social network theory and focuses on determinants of relational exchange. The findings support the social network argument that the presence of verbal agreements between business partners strongly and positively affects exchange relationships. Asset specificity and competition among buyers also affects such relationships. The results and their implications at the management and policy-making level are discussed in detail

  11. The occurrence of postharvest diseases on apples resistant to scab

    OpenAIRE

    Hanna Bryk; Dorota Kruczyńska

    2012-01-01

    The occurrence of storage diseases on fruit of seven scab resistant apple cultivars (Freedom, Rajka, Topaz, Rubinola, Enterprise, Goldstar, GoldRush) grafted on M.9 was investigated in 2001-2005. The trees were planted in 1995. It was found that after storage (4 and 6 months at 2°C, 85-90% RH) the most severe appeared to be bull's eye rot (Pezicula spp.). The most sensitive cultivars to this disease were: Topaz, Freedom, Goldstar, the least sensitive were Rubinola, Enterprise, Rajka. Other po...

  12. An Apple II -based bidimensional pulse height analyser

    International Nuclear Information System (INIS)

    The implementation of a pulse height analyser function in an Apple II microcomputer using minimal purpose built hardware is described. Except for a small interface module the system consists of two suites of software, one giving a conventional one dimensional analysis on a span of 1024 channels, and the other a two dimensional analysis on a 128 x 128 image format. Using the recently introduced ACCELERATOR coprocessor card the system performs with a dead time per event of less than 50 μS. Full software facilities are provided for display, storage and processing of the data using standard Applesoft BASIC. (author)

  13. Onshoring: An i-Opener for Apple, Inc.

    OpenAIRE

    Mr. Francisco Javier Salazar; Dr. Ryan Lunsford

    2014-01-01

    The December 2012 announcement from Apple, Inc.’s CEO Tim Cook that the company intends to move the production of its Mac computer lines to the United States is interesting on several levels. Cook acknowledged that the move is influenced, at least in part, by a desire to bolster a sluggish U.S. unemployment rate that continues to hover around 8% (Gross, 2012). There was no mention of where the company might be locating its facilities at the time of the announcement, “but bringing assembly-lin...

  14. RAČUNALNIŠTVO V OBLAKU PODJETJA APPLE INC. - ICLOUD

    OpenAIRE

    Pečenko, Jan

    2013-01-01

    V tem diplomskem seminarju je obravnavana tema računalništvo v oblaku in sicer na primeru storitve iCloud podjetja Apple Inc., ki je namenjena vsem uporabnikom Applovih produktov(računalnikov in mobilnih naprav). V prvem delu smo podrobneje predstavili teorijo računalništva v oblaku, ki predstavlja novo tehnologijo, s katero je uporabnikom (podjetjem in posameznikom) omogočeno shranjevanje podatkov v splet in dostopanje do njih kadarkoli in kjerkoli s katerokoli napravo enostavno s pomo...

  15. Modeling Apple Surface Temperature Dynamics Based on Weather Data

    OpenAIRE

    Lei Li; Troy Peters; Qin Zhang; Jingjin Zhang; Danfeng Huang

    2014-01-01

    The exposure of fruit surfaces to direct sunlight during the summer months can result in sunburn damage. Losses due to sunburn damage are a major economic problem when marketing fresh apples. The objective of this study was to develop and validate a model for simulating fruit surface temperature (FST) dynamics based on energy balance and measured weather data. A series of weather data (air temperature, humidity, solar radiation, and wind speed) was recorded for seven hours between 11:00–18:00...

  16. Management and Performance of APPLE Battery in High Temperature Environment

    Science.gov (United States)

    Suresh, M. S.; Subrahmanyam, A.; Agrawal, B. L.

    1984-01-01

    India's first experimental communication satellite, APPLE, carried a 12 AH Ni-Cd battery for supplying power during eclipse. Failure to deploy one of the two solar panels resulted in the battery operating in a high temperature environment, around 40 C. This also resulted in the battery being used in diurnal cycles rather than just half yearly eclipse seasons. The management and performance of the battery during its life of two years are described. An attempt to identify the probable degradation mechanisms is also made.

  17. Discovery of three woolly apple aphid Eriosoma lanigerum (Hemiptera: Aphididae) biotypes in Australia: the role of antixenosis and antibiosis in apple tree resistance

    Czech Academy of Sciences Publication Activity Database

    Costa, Arnaud; Williams, D. G.; Powell, K. S.

    2014-01-01

    Roč. 53, č. 3 (2014), s. 280-287. ISSN 2052-1758 Institutional support: RVO:60077344 Keywords : aphid * apple * biotype Subject RIV: EH - Ecology, Behaviour http://onlinelibrary.wiley.com/doi/10.1111/aen.12074/pdf

  18. Evaluation of the {sup 14}C-prochloraz residue levels in irradiated mangoes (Mangifera indica) and apples (Malus domestica).; Avaliacao dos niveis de residuos de {sup 14}C-prochloraz em mangas (Mangigera indica) irradiadas e em macas (Malus domestica)

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Maria Aparecida

    1998-07-01

    The Brazilian crops mangoes and apples has expanding in the last years. However, tropical fruits crops are susceptible to infection that harms the crop yield. The control of these diseases is made through fungicides such as prochloraz which possesses protecting eradicating action by controlling anthracnose in mangoes and scabies in apples. Agrochemicals are of great importance in the agriculture considering the of relationship cost/benefit. However they may cause a series of problems in the ecosystem, being the levels of agrochemicals residues in fruits one of these factors. The aim of this work was to evaluate the prochloraz levels in mangoes treated in both stages of pre and post harvest, and in apples treated in the pre harvest. In the treatment of the mangoes, the interaction, fungicide with the gamma radiation with dose of 1,0 kGy, was used to induce prochloraz degradation. Treated post harvest mangoes were stored, at 12 deg C during 21 days. The results showed that the prochloraz did not present reduction in the residual levels of the mangoes after 21 day storage, that is the safe period established by the Brazilian legislation on agrochemicals, in treated mangoes. The refrigerated storage (12 deg C) and the gamma radiation also did not contribute to the degradation of the fungicide in mangoes. In average the concentration of the fungicide prochloraz in the peels (bar X = 1,64 mug/g) was higher than in the mangoes pulp (bar x = 0,06 mug/g), which allows the consumption of this fruit, since the peel is always discarded. The degradation product, formed in peels of mangoes in fruits treated in the pre and post harvest was the metabolite BIS 44596; for mangoes treated in the pre-post harvest the metabolite formed was the BIS 445186. Both metabolites were found in very low levels, confirming that occurs degradation of the fungicide in mangoes. In apples treated with the prochloraz, reduction of 500/0 in the levels. (author)

  19. Edible Coating as Carrier of Antimicrobial Agents to Extend the Shelf Life of Fresh-Cut Apples

    Science.gov (United States)

    Edible coatings with antimicrobial agents provide a novel way to improve the safety and shelf-life of fresh-cut fruit. The effect of lemongrass, oregano oil and vanillin, incorporated in apple puree-alginate edible coatings, on the shelf-life of fresh-cut Fuji apples, was investigated. Coated appl...

  20. Phenolic Compounds in Apple (Malus x domestica Borkh.): Compounds Characterization and Stability during Postharvest and after Processing

    OpenAIRE

    Alessandra Francini; Luca Sebastiani

    2013-01-01

    This paper summarizes the information on the occurrence of phenolic compounds in apple (Malus x domestica Borkh.) fruit and juice, with special reference to their health related properties. As phytochemical molecules belonging to polyphenols are numerous, we will focus on the main apples phenolic compounds with special reference to changes induced by apple cultivar, breeding approaches, fruit postharvest and transformation into juice.

  1. Transcriptome changes in apple peel tissues during CO2 injury symptom development under controlled atmosphere storage regimens

    Science.gov (United States)

    Apple (Malus × domestica Borkh.) is one of the most widely cultivated tree crops, and fruit storability is vital to the profitability of the apple fruit industry. Fruit of many apple cultivars can be stored for an extended period due to the introduction of advanced storage technologies such as cont...

  2. Using AppleWorks V1.3 To Format Data Disks for Use with the Apple IIe Computer. Second Edition.

    Science.gov (United States)

    Schlenker, Richard M.

    This step-by-step guide to using AppleWorks V1.3 to format data disks for use with the Apple IIe computer covers (1) program loading; (2) selecting the add files menu; (3) selecting a different disk; (4) selecting disk 2; (5) returning to the main menu; (6) selecting other activities; (7) disk formatting; (8) volume naming; (9) entering the disk…

  3. Pectin modifications and the role of pectin-degrading enzymes during postharvest softening of Jonagold apples.

    Science.gov (United States)

    Gwanpua, Sunny George; Van Buggenhout, Sandy; Verlinden, Bert E; Christiaens, Stefanie; Shpigelman, Avi; Vicent, Victor; Kermani, Zahra Jamsazzadeh; Nicolai, Bart M; Hendrickx, Marc; Geeraerd, Annemie

    2014-09-01

    This study aimed at understanding softening in Jonagold apple (Malus×domestica Borkh.) fruits, by investigating pectin modifications and the evolution of pectin-modifying enzymes during postharvest storage and ripening. Jonagold apples were harvested at commercial maturity and stored at different temperatures and controlled atmosphere conditions for 6 months, followed by exposure to ambient shelf life conditions (20 °C under air) for 2 weeks. The composition of the pectic material was analysed. Furthermore, the firmness and the ethylene production of the apples were assessed. Generally, the main changes in pectin composition associated with the loss of firmness during ripening in Jonagold apples were a loss of side chains neutral sugars, increased water solubility and decreased molar mass. Also, the activities of four important enzymes possibly involved in apple softening, β-galactosidase, α-arabinofuranosidase, polygalacturonase and pectin methylesterase, were measured. Pectin-related enzyme activities highly correlated with ethylene production, but not always with pectin modifications. PMID:24731343

  4. Genome-wide analysis of the synonymous codon usage patterns in apple

    Institute of Scientific and Technical Information of China (English)

    LI Ning; SUN Mei-hong; JIANG Ze-sheng; SHU Huai-rui; ZHANG Shi-zhong

    2016-01-01

    Apple (Malus×domestica) has been proposed as an important woody plant and the major cultivated fruit trees in temperate regions. Apple whole genome sequencing has been completed, which provided an excelent opportunity for genome-wide analysis of the synonymous codon usage patterns. In this study, a multivariate bioinformatics analysis was performed to reveal the characteristics of synonymous codon usage and the main factors affecting codon bias in apple. The neutrality, correspondence, and correlation analyses were performed by CodonW and SPSS (Statistical Product and Service Solu-tions) programs, indicating that the apple genome codon usage patterns were affected by mutational pressure and selective constraint. Meanwhile, coding sequence length and the hydrophobicity of proteins could also inlfuence the codon usage patterns. In short, codon usage pattern analysis and determination of optimal codons has laid an important theoretical basis for genetic engineering, gene prediction and molecular evolution studies in apple.

  5. EVALUATION OF RESISTANCE TO PESTS AND DISEASES OF SOME OLD APPLE VARIETIES

    OpenAIRE

    Militaru Madalina; Butac Madalina; Sumedrea Mihaela; Călinescu Mirela; Marin F. C.

    2015-01-01

    Old apple varieties (ˈBotaneˈ, ˈCălugăreştiˈ, ˈDomneştiˈ, ˈCreţesc auriuˈ, ˈGustav durabilˈ, ˈPătulˈ, ˈRoşii de Geoagiuˈ, ˈŞovari, ˈVerzişoareˈ, ˈTare de ghindăˈ, ˈVerzi de Rădăşeniˈ, ˈPoinicˈ) were studied in order to assess pests and disease resistance during two growing season. During the experiment, the following disease and pest evaluations were made periodically: apple powdery mildew, apple scab, green apple aphid, apple codling moth, in a plot with low pesticide regime. High scab...

  6. Urinary estrogen metabolites and breast cancer

    DEFF Research Database (Denmark)

    Dallal, Cher M; Stone, Roslyn A; Cauley, Jane A;

    2013-01-01

    Background: Circulating estrogens are associated with increased breast cancer risk, yet the role of estrogen metabolites in breast carcinogenesis remains unclear. This combined analysis of 5 published studies evaluates urinary 2-hydroxyestrone (2-OHE1), 16a-hydroxyestrone (16a-OHE1), and their...... ratio (2:16a-OHE1) in relation to breast cancer risk. ¿Methods: Primary data on 726 premenopausal women (183 invasive breast cancer cases and 543 controls) and 1,108 postmenopausal women (385 invasive breast cancer cases and 723 controls) were analyzed. Urinary estrogen metabolites were measured using...... enzyme linked immunosorbent assays. Study-specific and combined multivariable adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were estimated based on tertiles of estrogen metabolites. Multinomial logistic regression models were fit according to hormone receptor status.¿Results: Higher...

  7. Isotope analysis (δ13C of pulpy whole apple juice

    Directory of Open Access Journals (Sweden)

    Ricardo Figueira

    2011-09-01

    Full Text Available The objectives of this study were to develop the method of isotope analysis to quantify the carbon of C3 photosynthetic cycle in pulpy whole apple juice and to measure the legal limits based on Brazilian legislation in order to identify the beverages that do not conform to the Ministry of Agriculture, Livestock and Food Supply (MAPA. This beverage was produced in a laboratory according to the Brazilian law. Pulpy juices adulterated by the addition of sugarcane were also produced. The isotope analyses measured the relative isotope enrichment of the juices, their pulpy fractions (internal standard and purified sugar. From those results, the quantity of C3 source was estimated by means of the isotope dilution equation. To determine the existence of adulteration in commercial juices, it was necessary to create a legal limit according to the Brazilian law. Three brands of commercial juices were analyzed. One was classified as adulterated. The legal limit enabled to clearly identify the juice that was not in conformity with the Brazilian law. The methodology developed proved efficient for quantifying the carbon of C3 origin in commercial pulpy apple juices.

  8. Apple shoot multiplication and plantlets reaction to in vitro culture

    Directory of Open Access Journals (Sweden)

    Anca BUTIUC-KEUL

    2010-05-01

    Full Text Available The present work aimed to evaluate the expression of several enzymatic systems in apple (Malus domestica Borkh., cvs. Florina, Romus3, Romus4, Colmar, Rebra, Goldrush, Idared plants grown in vitro in comparison with the in vivo donor plants. In vitro culture was established on Murashige and Skoog (1962 basal medium supplemented with Lee and Fossard (1977 (LF vitamins, 2 mg l-1 N6-benzyladenine, 0.01 mg l-1 N6-naphtyl-acetic acid, 30 g l-1 dextrose and 7 g l-1 agar. The highest shoot proliferation was obtained for all cultivars on medium supplemented with 1.0 mg/l N6-benzyladenine. Our study shows that in vivo plants have a distinct pattern of izoesterases in comparison with in vitro plantlets. Several izoesterases characteristic for in vitro or in vivo plants were identified. Izoperoxidases are inducible with culture conditions, physiological condition and developmental stage. The pattern of superoxid-dismutases is less variable with the culture conditions which demonstrate that in vitro culture does not occur oxidative stress. According to the pattern of peroxidases, estarases and superoxid-dismutases, there are not significant differences between in vivo and in vitro plants. Valuable apple cultivars could be preserved short or medium term by in vitro culture without genetically changes.

  9. Preparation of an acid butanol standard from fresh apples.

    Science.gov (United States)

    Li, Chunmei; Trombley, John D; Schmidt, Michael A; Hagerman, Ann E

    2010-05-01

    We have developed a simple method for preparing and verifying suitable standards for the acid butanol assay from a readily available source. Phenolics were extracted from fresh apples with methanol, and sugars were removed from the crude extract by treatment with Amberlite resin before fractionating the proanthocyanidins into ethyl acetate. The ethyl acetate fraction was chromatographed on Toyopearl TSK HW-50F to yield about 50 mg of procyanidin dimer and 35 mg of trimer from 1 kg fresh apple fruit. The purity and identity of the standards was easily confirmed by using ESI-MS. In the acid butanol assay, the pure dimer, trimer and purified Sorghum procyanidin had similar color yields on a mass basis, and produced about three times more color than purified quebracho tannin. This new standard overcomes problems associated with overestimation of tannin due to use of the unreactive quebracho tannin standard. Use of the new standard will enable accurate comparisons of tannin levels between laboratories and will standardize comparisons between species, thus promoting our understanding of the role of condensed tannins in plants. PMID:20379766

  10. Analysis of the Volatile Constituents of Irradiated Apple Juice

    International Nuclear Information System (INIS)

    The organoleptic studies and wholesomeness tests that are being carried out as part of the International Programme on the Irradiation of Fruit and Fruit Juices (Seibersdorf Project) entail analysis of the aromatic substances present in irradiated and non-irradiated juice. The volatile substances present in irradiated fruit juices were analysed by gas chromatography, with direct injection of the emitted vapours at ambient temperature and at 60°C (Weurman's Head Space Technique). The volatile constituents were identified by comparing the amounts retained in the column with those for pure substances and by removing certain constituents from the vapour with the help of chemical reagents. To simplify the analyses, the first tests were carried out on concentrated apple juice from which the volatile substances had been removed before irradiation. Irradiation gave rise to five aldehydes in the normal apple juice (acetaldehyde, isobutyraldehyde, butyraldehyde, isovaldehyde and capronaldehyde), but only three in the concentrated juice (acetaldehyde, isobutyraldehyde and isovaleraldehyde). In addition, 2-butanone appeared in the concentrated juice; however, the peak corresponding to it on the chromatogram was completely masked by the ethanol peak in the case of non-concentrated juice. Furan was also detected, together with traces of two compounds that have not yet been identified. Similar results have been obtained by pasteurization, such as in bottling by heat. (author)

  11. Fractal dimension and mechanism of aggregation of apple juice particles.

    Science.gov (United States)

    Benítez, E I; Lozano, J E; Genovese, D B

    2010-04-01

    Turbidity of freshly squeezed apple juice is produced by a polydisperse suspension of particles coming from the cellular tissue. After precipitation of coarse particles by gravity, only fine-colloidal particles remain in suspension. Aggregation of colloidal particles leads to the formation of fractal structures. The fractal dimension is a measure of the internal density of these aggregates and depends on their mechanism of aggregation. Digitized images of primary particles and aggregates of depectinized, diafiltered cloudy apple juice were obtained by scanning electron microscopy (SEM). Average radius of the primary particles was found to be a = 40 ± 11 nm. Maximum radius of the aggregates, R(L), ranged between 250 and 7750 nm. Fractal dimension of the aggregates was determined by analyzing SEM images with the variogram method, obtaining an average value of D(f) = 2.3 ± 0.1. This value is typical of aggregates formed by rapid flocculation or diffusion limited aggregation. Diafiltration process was found to reduce the average size and polydispersity of the aggregates, determined by photon correlation spectroscopy. Average gyration radius of the aggregates before juice diafiltration was found to be R(g) = 629 ± 87 nm. Average number of primary particles per aggregate was calculated to be N = 1174. PMID:21339133

  12. Transporter and its engineering for secondary metabolites.

    Science.gov (United States)

    Lv, Huajun; Li, Jianhua; Wu, Yingying; Garyali, Sanjog; Wang, Yong

    2016-07-01

    Secondary metabolites possess a lot of biological activities, and to achieve their functions, transmembrane transportation is crucial. Elucidation of their transport mechanisms in the cell is critical for discovering ways to improve the production. Here, we have summarized the recent progresses for representative secondary metabolite transporters and also the strategies for uncovering the transporter systems in plants and microbes. We have also discussed the transporter engineering strategies being utilized for improving the heterologous natural product production, which exhibits promising future under the guide of synthetic biology. PMID:27209041

  13. [Autism and Autism-associated Metabolites].

    Science.gov (United States)

    Watanabe, Kunitomo

    2016-06-01

    Gene-microbiota interactions are now proposed to be a special case of gene-environmental interaction. Preclinical and clinical data summarized in this article reveal that a specific serum metabolite, associated with alterations in gut microbiome composition, might have an emerging role in the onset and pathogenesis of autism. Altered level of this specified metabolite may induce perturbations in the epigenome and modulate the expression of key disease susceptible genes in neurons and their associated cells during critical periods of neurodevelopment. The gut microbiota itself is now regarded as a reservoir for environmental epigenetic factors. PMID:27279160

  14. Animal bioavailability of defined xenobiotic lignin metabolites

    International Nuclear Information System (INIS)

    Lignin has been recognized as a major component of bound pesticide residues in plants and is thought to be undigestible in animals. Two defined ring-U-14C-labeled chloroaniline/lignin metabolites have now been fed to rats, where a release of ∼66% of the bound xenobiotic occurred in the form of simple chloroaniline derivatives. The observed high degree of bioavailability indicates that bound pesticidal residues may possess ecotoxicological significance. In parallel studies, the white-rot fungus Phanerochaete chrysosporium was more efficient, and a soil system was much less efficient, in the degradation of the [ring-U-14C]chloroaniline/lignin metabolites

  15. Metabolites from mangrove endophytic fungus Dothiorella sp.

    Institute of Scientific and Technical Information of China (English)

    XUQingyan; WANGJianfeng; HUANGYaojian; ZHENGZhonghui; SONGSiyang; ZHANGYongmin; SUWenjin

    2004-01-01

    Mangroves are special woody plant communities in the intertidal zone of tropical and subtropical coasts. They prove to be a natural microorganisms and new metabolites storage. In the study of mangrove endophytic fungi metabolites, four new compounds, Compounds 1, 2, 3 and 4, as well as a known octaketide, cytosporone B (5), are isolated from an endophytic fungus, Dothiorella sp., HTF3. They all show cytotoxic activities. The elucidation of these structures is mainly based on 1D/2D NMR and ESI-MS spectral analyses.

  16. In vitro characterization of the impact of different substrates on metabolite production, energy extraction and composition of gut microbiota from lean and obese subjects.

    Directory of Open Access Journals (Sweden)

    Marisol Aguirre

    Full Text Available The aim of this study was to investigate the effect of galacto-oligosaccharides, lactulose, apple fiber and sugar beet pectin on the composition and activity of human colonic microbiota of lean and obese healthy subjects using an in vitro model of the proximal colon: TIM-2. Substrate fermentation was assessed by measuring the production of short-chain and branched-chain fatty acids, lactate and ammonia and by studying the composition of the bacterial communities over time. The results suggest that energy harvest (in terms of metabolites of lean and obese microbiotas is different and may depend on the fermentable substrate. For galacto-oligosaccharides and lactulose, the cumulative amount of short-chain fatty acids plus lactate produced in TIM-2 was lower in the fermentation experiments with the lean microbiota (123 and 155 mmol, respectively compared to the obese (162 and 173 mmol, respectively. This was reversed for the pectin and the fiber. The absolute amount produced of short-chain fatty acids including lactate was higher after 72 h in the fermentation experiments with apple fiber-L (108 mmol than with apple fiber-O (92 mmol. Sugar beet-L was also higher (130 mmol compared to sugar beet-O (103 mmol. Galacto-oligosaccharides and lactulose boosted the balance of health-promoting over toxic metabolites produced by the microbiota from obese subjects. Firmicutes were more predominant in the inoculum prepared from feces of obese subjects compared to lean subjects. The average abundance at time zero was 92% and 74%, respectively. On the other hand, Bacteroidetes were more dominant in the microbiota prepared with homogenates from lean subjects with an average abundance of 22% compared with the microbiota prepared with homogenates from obese subjects (3.6%. This study brings evidence that different fermentable carbohydrates are fermented differently by lean and obese microbiotas, which contributes to the understanding of the role of diet and the

  17. In vitro characterization of the impact of different substrates on metabolite production, energy extraction and composition of gut microbiota from lean and obese subjects.

    Science.gov (United States)

    Aguirre, Marisol; Jonkers, Daisy M A E; Troost, Freddy J; Roeselers, Guus; Venema, Koen

    2014-01-01

    The aim of this study was to investigate the effect of galacto-oligosaccharides, lactulose, apple fiber and sugar beet pectin on the composition and activity of human colonic microbiota of lean and obese healthy subjects using an in vitro model of the proximal colon: TIM-2. Substrate fermentation was assessed by measuring the production of short-chain and branched-chain fatty acids, lactate and ammonia and by studying the composition of the bacterial communities over time. The results suggest that energy harvest (in terms of metabolites) of lean and obese microbiotas is different and may depend on the fermentable substrate. For galacto-oligosaccharides and lactulose, the cumulative amount of short-chain fatty acids plus lactate produced in TIM-2 was lower in the fermentation experiments with the lean microbiota (123 and 155 mmol, respectively) compared to the obese (162 and 173 mmol, respectively). This was reversed for the pectin and the fiber. The absolute amount produced of short-chain fatty acids including lactate was higher after 72 h in the fermentation experiments with apple fiber-L (108 mmol) than with apple fiber-O (92 mmol). Sugar beet-L was also higher (130 mmol) compared to sugar beet-O (103 mmol). Galacto-oligosaccharides and lactulose boosted the balance of health-promoting over toxic metabolites produced by the microbiota from obese subjects. Firmicutes were more predominant in the inoculum prepared from feces of obese subjects compared to lean subjects. The average abundance at time zero was 92% and 74%, respectively. On the other hand, Bacteroidetes were more dominant in the microbiota prepared with homogenates from lean subjects with an average abundance of 22% compared with the microbiota prepared with homogenates from obese subjects (3.6%). This study brings evidence that different fermentable carbohydrates are fermented differently by lean and obese microbiotas, which contributes to the understanding of the role of diet and the microbiota in

  18. Ecohydrological interactions between soil and trees in Alpine apple orchards

    Science.gov (United States)

    Penna, Daniele; Scandellari, Francesca; Zanotelli, Damiano; Michael, Engel; Tagliavini, Massimo; Comiti, Francesco

    2016-04-01

    Tracer-based investigations of water exchanges between soil and trees in natural forested catchments are receiving relevant attention in modern ecohydrology. However, the interactions between tree water use and the hydrological cycle in agricultural environments are still poorly understood. In this work, we use stable isotopes of water (2H and 18O) and electric conductivity as tracers to improve our understanding of the functional interrelations between water generating surface runoff and recharging groundwater, and water taken up by apple trees (Malus domestica, cv. 'Pinova') in an Alpine valley in South Tyrol, Northern Italy. From April to October 2015 we monitored two orchards approximately of the same size (roughly 400 m2) and soil texture (silt loam) located in a flat area at different distance from the Adige/Etsch River (50 m vs. 450 m). We have addressed the following questions: i) at which soil depth do apple trees take up water? ii) do apple trees take up water from shallow groundwater? iii) are there differences in the isotopic composition of the water fluxes between the two sites? Samples for isotopic analysis were taken approximately fortnightly from the river, two groundwater wells close to each field, mobile soil water (from suction cups at 25 cm and 50 cm), open area precipitation, throughfall, irrigation and sap (through a portable pressure bomb). Tightly-bound soil water was also cryogenically extracted from samples taken every 10 cm from 60 cm-long soil cores taken at three locations for each field on one occasion in mid-summer. Ancillary measurements were electrical conductivity of all water sources except for sap. In addition to meteorological and discharge data, soil moisture was continuously measured at 10 cm and 50 cm in three locations, and sap flow on three trees, for each field. Preliminary results show that two water pools with distinct isotopic signature exist: i) river water, groundwater and irrigation water show values relatively

  19. Human pharmacokinetics of proguanil and its metabolites

    DEFF Research Database (Denmark)

    Bygbjerg, Ib Christian; Ravn, P; Rønn, A;

    1987-01-01

    The pharmacokinetics of proguanil and its metabolites cycloguanil and p-chlorophenylbiguanide were studied in five healthy volunteers taking 200 mg orally for 14 days. A highly sensitive and specific high-performance liquid chromatographic assay was applied, clearly identifying all three compounds...

  20. Secondary metabolites from Scorzonera latifolia roots

    Czech Academy of Sciences Publication Activity Database

    Acikara, O. B.; Šmejkal, K.; Cvačka, Josef; Buděšínský, Miloš; Dračínský, Martin; Saltan, G.

    2015-01-01

    Roč. 81, č. 16 (2015), PM167. ISSN 0032-0943. [GA 2015. International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research /63./. 23.08.2015-27.08.2015, Budapest] Institutional support: RVO:61388963 Keywords : medical plant * metabolites * Asteraceae Subject RIV: CB - Analytical Chemistry, Separation

  1. Analysis of 'Fuji' apple somatic variants from next-generation sequencing.

    Science.gov (United States)

    Lee, H S; Kim, G H; Kwon, S I; Kim, J H; Kwon, Y S; Choi, C

    2016-01-01

    The domesticated apple (Malus x domestica Borkh.) is a major fruit crop of temperate regions of the world. 'Fuji' apple (Ralls Genet x Delicious), a famous apple cultivar in Korea, has been very popular since its promotion in Japan in 1958. 'Fuji' and its bud mutant cultivars possess variable levels of genetic diversity. Nonetheless, the phenotypes of each group, which are classified into the bud mutation groups: early season, fruiting spur, and coloring, are similar. Despite attempts to identify these bud mutation cultivars, molecular markers, which were developed before the emergence of next-generation sequencing technology, have not been able to distinguish each cultivar easily. In this study, we adopted the resequencing technique using the 'Golden Delicious' (Grimes Golden x Unknown) apple genome as a reference. SNPs (single nucleotide polymorphisms) and InDels (insertions or deletions) of 'Fuji' apple and its bud mutant cultivar were detected and SNPs and unique InDels distinct to each cultivar were identified. Data from this study may be used to identify bud mutant cultivars of 'Fuji' apples and be useful for further breeding of apples. PMID:27525934

  2. Patulin distribution in Fuji and Golden apples contaminated with Penicillium expansum.

    Science.gov (United States)

    Marín, S; Morales, H; Hasan, H A H; Ramos, A J; Sanchis, V

    2006-12-01

    This work assesses the extent of patulin contamination in Penicillium expansum-infected apples stored at room temperature for short periods of time and its relationship with apple variety (Golden or Fuji), degree of ripeness and size of lesions. Inoculated apples were incubated at 20 degrees C. Patulin was determined in both sound and decayed tissue from cylindrical samples taken around the lesions and cut into 0.5-cm thick sections. Higher accumulation of patulin occurred in Golden apples, with less ripened apples showing higher concentrations. Total accumulated patulin was similar or higher in 4-cm compared to 2-cm lesioned apples, although a decrease in patulin concentration was observed in older lesion sections. Patulin accumulation occurred over a short period of time at room temperature, thus the stand-by period before processing should be minimised. Of total patulin, 2-6% migrated to the surrounding sound tissue, thus trimming tissue around the rotten part may be a good preventive practice for apple derivative production. PMID:17118875

  3. Biological Control of Apple Anthracnose by Paenibacillus polymyxa APEC128, an Antagonistic Rhizobacterium.

    Science.gov (United States)

    Kim, Young Soo; Balaraju, Kotnala; Jeon, Yongho

    2016-06-01

    The present study investigated the suppression of the disease development of anthracnose caused by Colletotrichum gloeosporioides and C. acutatum in harvested apples using an antagonistic rhizobacterium Paenibacillus polymyxa APEC128 (APEC128). Out of 30 bacterial isolates from apple rhizosphere screened for antagonistic activity, the most effective strain was APEC128 as inferred from the size of the inhibition zone. This strain showed a greater growth in brain-heart infusion (BHI) broth compared to other growth media. There was a reduction in anthracnose symptoms caused by the two fungal pathogens in harvested apples after their treatment with APEC128 in comparison with non-treated control. This effect is explained by the increased production of protease and amylase by APEC128, which might have inhibited mycelial growth. In apples treated with different APEC128 suspensions, the disease caused by C. gloeosporioides and C. acutatum was greatly suppressed (by 83.6% and 79%, respectively) in treatments with the concentration of 1 × 10(8) colony forming units (cfu)/ml compared to other lower dosages, suggesting that the suppression of anthracnose development on harvested apples is dose-dependent. These results indicated that APEC128 is one of the promising agents in the biocontrol of apple anthracnose, which might help to increase the shelf-life of apple fruit during the post-harvest period. PMID:27298600

  4. Analysis Method for Pesticides Residues by GC/MS in Lebanese Apple

    International Nuclear Information System (INIS)

    The apple's crop can be affected by many pests during the growing season, which requires careful monitoring. Both apple fruit and apple tree need to be treated by pesticides in order to protect them from pests. Such treatment often leads to the accumulation of stable pesticides inside the fruit. The local market provides a large variety of pesticides allowing farmers to use more than one active substance in order to protect their crop, often without proper advice. Monitoring pesticides on apples and other agricultural crops is the best way to protect consumers health from the hazards of pesticides residues. The development of new, rapid and effective method to analyze the multi pesticides residues at trace levels in apple samples is essential. This work describes the extraction procedure and the analytical method developed to detect the pesticide residues using the gas-chromatographic-mass spectrometric approach (GC-MS). The developed method was successfully applied to analyze apple samples collected from different Lebanese markets for a one year period in order to monitor the presence of pesticides and their stability in apple fruits during storage. (author)

  5. Determination of extractable organohalogens in apple by neutron activation analysis and gas chromatography

    International Nuclear Information System (INIS)

    In addition to compounds such as dichlorodiphenyl trichloroethanes (DDTs) and polychlorinated biphenyls (PCBs), which originate from by now well-known anthropogenic sources, there is increasing awareness of other halogenated compounds coming from a number of different processes, and probably also from natural source. To provide the reliable results and data for evaluation of hazard of extractable persistent organohalogen (EPOX) to food safety and to further characterize halogenated compounds in fruits, the concentrations of extractable organohalogen (EOX), EPOX, and identified organochlorine compounds including DDTs, hexachlorinated cyclohexanes (HCHs), chlordanes and PCBs in apple samples from Chinese homegrown and imports were determined by neutron activation analysis (NAA) and gas chromatography. The results indicated that the levels of EPOX in apples was in the order of EPOCl >> EPOBr > EPOI, which showed that EPOCl was the major fraction of the EPOX pollutions. Also, most chlorinated compounds in apple samples existed as inorganic species and water-soluble polar compounds, and about 1/10-4/5 of EOX was sulfuric acid-resistant EPOX contaminants. Meanwhile, about more than 94 % of EPOCl in detected specimens cannot be accounted for by DDTs, HCHs, chlordanes and PCBs. Furthermore, the T-test result indicated that the EPOCl levels in apple were significantly higher than those in peeled apple , which stated that people consuming peeled apple will get lesser intakes of EPOCl pollutants than they consume unpeeled apple.

  6. Variation in phenolic compounds and antioxidant activity in apple seeds of seven cultivars.

    Science.gov (United States)

    Xu, Ying; Fan, Mingtao; Ran, Junjian; Zhang, Tingjing; Sun, Huiye; Dong, Mei; Zhang, Zhe; Zheng, Haiyan

    2016-05-01

    Polyphenols are the predominant ingredients in apple seeds. However, few data are available on the phenolic profile or antioxidant activity in apple seeds in previous researches. In this study, low-molecular-weight phenolic compounds and antioxidant activity in seeds, peels, and flesh of seven apple cultivars grown in northwest China were measured and analyzed using HPLC and FRAP, DPPH, ABTS assays, respectively. HPLC analysis revealed phloridzin as the dominant phenolic compound in the seeds with its contents being 240.45-864.42 mg/100 gDW. Total phenolic content (TPC) measured by the Folin-Ciocalteu assay in apple seed extracts of seven cultivars ranged from 5.74 (Golden Delicious) to 17.44 (Honeycrisp) mgGAE/gDW. Apple seeds showed higher antioxidant activity than peels or flesh; antioxidant activity in seeds varied from 57.59 to 397.70 μM Trolox equivalents (TE)/g FW for FRAP, from 37.56 to 64.31 μM TE/g FW for DPPH, and from 220.52 to 708.02 μM TE/g FW for ABTS. TPC in apple seeds was significantly correlated with all three assays. Principal component analysis (PCA) indicated that Honeycrisp was characterized with high contents of total polyphenols and phloridzin. Our findings suggest that phenolic extracts from apple seeds have good commercial potential as a promising antioxidant for use in food or cosmetics. PMID:27081364

  7. Effect of boron fertilization of apple trees (Malus domestica Borth. on yield and fruit quality

    Directory of Open Access Journals (Sweden)

    Paweł Wójcik

    2013-12-01

    Full Text Available Aim of this work was to examine effect of boron fertilization on yield of apple trees and fruit quality. The experiment was carried out during 1994-1996 in Dąbrowice Experimental Orchard belonging to Research Institute of Pomology and Floriculture in Skiemiewice, on Š a m p i o n cultivar, grafted on M26 rootstock. Trees were planted in the autumn of 1991 at the distance 4 x 2,5 m, on sandy-loam soil. Before starting and during carrying out the study there were not visual symptoms of boron deficiency. In the experiment applied boron fertilization to the soil at the dose 2 g B per tree or three times boron sprays before or after bloom at the dose 0,67 g B per tree. Apple trees without any boron fertilization were served as a control. Results of experiment showed that only boron sprays after bloom increased fruit set and yield. There were no significant differences between treatments in fruit maturity at harvest, fruit weight losses dunng storage, apple number infected by Penicillium, Monilina and Botrytis cinerea. Boron sprays after bloom increased firmness of apples after storage and decreased sensibility to bitter pit, internal breakdown and Gloeosporium-rot. All boron treatments increased boron concentration in apples in comparison with control ones. However, the highest increase of boron concentration in apples was observed when boron sprays were done after bloom and soil boron application. Only boron sprays applied after bloom increased calcium concentration in apples.

  8. Metabolite coupling in genome-scale metabolic networks

    Directory of Open Access Journals (Sweden)

    Palsson Bernhard Ø

    2006-03-01

    Full Text Available Abstract Background Biochemically detailed stoichiometric matrices have now been reconstructed for various bacteria, yeast, and for the human cardiac mitochondrion based on genomic and proteomic data. These networks have been manually curated based on legacy data and elementally and charge balanced. Comparative analysis of these well curated networks is now possible. Pairs of metabolites often appear together in several network reactions, linking them topologically. This co-occurrence of pairs of metabolites in metabolic reactions is termed herein "metabolite coupling." These metabolite pairs can be directly computed from the stoichiometric matrix, S. Metabolite coupling is derived from the matrix ŜŜT, whose off-diagonal elements indicate the number of reactions in which any two metabolites participate together, where Ŝ is the binary form of S. Results Metabolite coupling in the studied networks was found to be dominated by a relatively small group of highly interacting pairs of metabolites. As would be expected, metabolites with high individual metabolite connectivity also tended to be those with the highest metabolite coupling, as the most connected metabolites couple more often. For metabolite pairs that are not highly coupled, we show that the number of reactions a pair of metabolites shares across a metabolic network closely approximates a line on a log-log scale. We also show that the preferential coupling of two metabolites with each other is spread across the spectrum of metabolites and is not unique to the most connected metabolites. We provide a measure for determining which metabolite pairs couple more often than would be expected based on their individual connectivity in the network and show that these metabolites often derive their principal biological functions from existing in pairs. Thus, analysis of metabolite coupling provides information beyond that which is found from studying the individual connectivity of individual

  9. Evaluation of the nutritive value of apple pulp mixed with different amounts of wheat straw

    OpenAIRE

    Rodrigues, M.A.M.; Guedes, C.M.; Rodrigues, A.; Cone, J.W.; Gelder, van, H.; Ferreira, L.M.M.

    2008-01-01

    Given the high amounts of apple rejected for commercialization its use as alternative feed for ruminants should be considered. This study was designed to investigate the nutritive value of apple pulp-wheat straw mixtures. Chemical composition, in vitro organic matter digestibility (IVOMD) and gas production profiles of ensiled mixtures containing 85 (M85), 70 (M70), and 50% (M50) of apple pulp were studied at 0, 15, 30 and 45 days. Fermentation quality was assessed by pH, lactic acid, ethanol...

  10. Apple-Design - Die Kunst der Produktgestaltung zwischen Userzentrierung und Ästhetik

    OpenAIRE

    Klinke, Harald

    2010-01-01

    Die Produkte der Firma Apple fallen durch die Schlichtheit ihres Designs auf. Apples umfassende Verständnis von Gestaltung und die damit zusammenhängende Integration von äußerer Gestaltung und technischer Umsetzung haben ihre Vorbilder unter anderem in Deutschland. Nach den Vorstellungen von Steve Jobs, dem Apple-Chef, sind Kunst und Technik keine Gegensätze mehr, sondern bedingen sich gegenseitig. Designer und Ingenieure werden vielmehr zu Künstlern, von denen die Impulse zur Produk...

  11. The apple pulp waste used as a nourishing base by Aspergillus niger

    OpenAIRE

    Mojsov, Kiro

    2011-01-01

    The aim of this work was to develop a low cost process for apple pulp utilization. This actual waste from food and agriculture industry was used as a nourishing base by Aspergillus niger MK-15. The apple pulp combined with corn flour and simple mineral salts was utilized as a nourishing base by submerged production of pectinolytic enzymes by the fungus Aspergillus niger MK-15. Different concentration on apple pulp (1%; 2%; and 3%, w/v) and different pH initial on the bases (4; 5 and 6) were s...

  12. Comparative morphological analysis of apple blister mite, Eriophyes mali Nal., a new pest in Serbia

    Directory of Open Access Journals (Sweden)

    Biljana Vidović

    2014-06-01

    Full Text Available The apple blister mite, Eriophyes mali Nalepa, 1926 (Acari: Prostigmata: Eriophyoidea, has been recently found in Serbia as a new pest of apple. The history of its research, the results of a morphological analysis and degree of infestation are presented. A comparison of the main morphological features of mites from different populations of remote geographical origin has shown that the apple blister mite from Serbia is most similar to another European population (Bulgarian [or Austrian?] while it differs from E. mali originating from the USA and New Zealand. The percentage of infestation varied from 1.6% to 87.6%, with an average of 22.4%.

  13. Screening of hypo virulence of Colletotrichum gloeosporioides and its control effect on apple anthracnose

    International Nuclear Information System (INIS)

    The Colletotrichum gloeosporioides is a nosogenesis important fungus that causes the serious economic loss on apple, however, inducing resistance of the host may reduce the loss. Colletotrichum gloeosporioides was treated by ion implantation and by alternating magnetic field to induce aberrance. It was screened through eyeballing first, and then screened through growth on apple. Two aberrance strains (C100-2-5 and C0.25-1-2 were selected. Both the strains have good protective function and good effect to control the apple disease. (authors)

  14. Leading Apple with Steve Jobs management lessons from a controversial genius

    CERN Document Server

    Elliot, Jay

    2012-01-01

    A former Senior VP of Apple shares how Steve Jobs motivated people to do the best work of their lives Jay Elliot was hired personally by Steve Jobs, just in time to accompany him on the last of his historic visits to Xerox's Palo Alto Research Center, the visits that changed the course of computing. As Senior VP of Apple, Jay served as Steve's right-hand man and trouble-shooter, overseeing all corporate operations and business planning, as well as software development and HR. In Leading Apple with Steve Jobs, Jay details how Steve managed and motivated his people-and what every

  15. Heat Transfer Convection in The Cooking of Apple Using a Solar Cooker Box-Type

    International Nuclear Information System (INIS)

    In this work, experimental results to determine the convection heat transfer coefficient in the cooking process of apple using a solar cooker box-type are presented. Experimental data of temperatures for water, surface and central point of the apple were used. To determine the convection coefficient, the apple was modelled as a sphere. The temperatures evolution was defined using thermocouples located at water, surface and central point in the vegetables. Using heat transfer convection equations in transitory state and the temperatures measured, the Biot number and the convection coefficient were determined

  16. Effective moisture diffusivity determination and mathematical modelling of drying curves of apple pomace

    Science.gov (United States)

    Kara, Cem; Doymaz, İbrahim

    2014-12-01

    Drying of apple pomace representing by-products from apple juice processing was studied. The results obtained show that moisture content of the pomace decreases with time and temperature. The Midilli et al. model was selected as the best mathematical model for describing the drying kinetics of the apple pomace. The effective moisture diffusivity varied from 1.73 × 10-10 to 4.40 × 10-10 m2/s and the activation energy was calculated to be 29.65 kJ/mol.

  17. Owning the consumer - getting to the core of the Apple business model

    OpenAIRE

    Montgomorie, Johnna; Roscoe, Samuel

    2013-01-01

    This paper uses a business model framework to analyze the main limitations of Apple Inc. post-2003, a significant turning point in the company's history. As such, we move beyond an exclusive focus on what makes Apple unique or different by evaluating the mundane and out-dated elements of its business model. To do so, we examine the end-to-end supply chain, from source to store, to present a more holistic evaluation of the Apple business model. Drawing on the existing literature, we argue that...

  18. Impact analysis of different chemical pre-treatments on colour of apple discs during drying process

    OpenAIRE

    Magdić, D.; Lukinac, Jasmina; Jokić, Stela; Čačić-Kenjerić, F.; Bilić, M.; Velić, D.

    2009-01-01

    The main purpose of this study was to compare colour changes of chemically pre-treated dried apple discs. Changes were observed by chromameter in L*a*b* colour model by using Minolta chromameter CR-400 and by image analysis system in RGB colour model. Apple discs variety "Gold Rush" were pre-treated and dried in laboratory tray drier at drying temperature 70 °C and at airflow velocity of 1.5 ms-1. Different chemical pre-treatments were applied on apple discs (dipping in 0.5% ascorbic acid sol...

  19. Effects of acidity of simulated rain on the fruiting of 'Summerred' apple trees

    International Nuclear Information System (INIS)

    The effects of rain acidity on field-grown Summered apple trees (Malus domestica Borkh) under natural conditions were investigated. One group of four trees was exposed to ambient rainfall. Four other groups were covered with rainshields and received water, pH 5.6, 4, and 3, respectively, as simulated rain. Simulated acid rain, particularly at pH 3, adversely affected fruit production in terms of individual fruit weight, fruit set, fruit appearance (necrosis and russetting of the peel) and dry weight. Ambient rain was not found to cause significant reductions in apple weight. Ambient rain was not found to cause significant reductions in apple fruit production in this study

  20. APPLE-II type quasi-periodic variably polarizing undulator at HiSOR

    International Nuclear Information System (INIS)

    A newly constructed quasi-periodic APPLE-II undulator was installed in the HiSOR ring at Hiroshima Synchrotron Radiation Center, Hiroshima University during the summer shutdown period in 2011. This 1.8 m-long undulator has a period length of 78 mm. In this article, the mechanism of magnetic field generation for various polarization modes of APPLE undulator, the principle of quasi-periodic undulator and the performance of HiSOR QP-APPLE-II undulator are described. (author)

  1. Apple Watch产业链看健康大数据

    Institute of Scientific and Technical Information of China (English)

    2014-01-01

    <正>Apple Watch已经送往美国医药管理局FDA医疗认证,代表设备未来可能介入医疗环节,替代部分检测仪器,配合医院提供医疗级别的检测服务,协助人们进行日常生活健康的管理。大数据支撑健康服务产业Apple Watch及其背后的庞大商业服务前景想象空间很大。Apple Watch+iPhone/iPad

  2. Owning the consumer: getting to the core of the Apple Business Model

    OpenAIRE

    Johnna Montgomerie and Samuel Roscoe

    2012-01-01

    This paper uses a business model framework to analyze the main limitations of Apple Inc. post-2003, a significant turning point in the company’s history. As such we move beyond an exclusive focus on what makes Apple unique or different by evaluating the mundane and out-dated elements of its business model. To do this we examine the end-to-end supply chain, from source to store, in order to put forward a more holistic evaluation of the Apple business model. Drawing on the existing business mod...

  3. QTL detection for physicochemical characteristics of cashew apple

    Directory of Open Access Journals (Sweden)

    Francisco Herbeth Costa dos Santos

    2011-08-01

    Full Text Available The identification of quantitative trait loci (QTL and marker-assisted selection have aroused great interest inbreeding programs aiming at fruit quality. The objective of this study was to detect QTL related to the quality of the cashew apple.The physicochemical characteristics oligomeric phenolics, total soluble solids, total titrable acidity and vitamin C contents wereanalyzed in the mapped cashew population. QTL were detected by QTL interval and multiple QTL mapping. The results showedhigh phenotypic variation in the segregating F1 generation for all traits. Eighteen QTL associated with cashew quality wereidentified: three for oligomeric phenolics, five for total soluble solids, six for total acidity and four for vitamin C. QTL are promisingfor marker-assisted selection since they have the greatest phenotypic effects and contribution to phenotypic variation.

  4. The occurrence of postharvest diseases on apples resistant to scab

    Directory of Open Access Journals (Sweden)

    Hanna Bryk

    2012-12-01

    Full Text Available The occurrence of storage diseases on fruit of seven scab resistant apple cultivars (Freedom, Rajka, Topaz, Rubinola, Enterprise, Goldstar, GoldRush grafted on M.9 was investigated in 2001-2005. The trees were planted in 1995. It was found that after storage (4 and 6 months at 2°C, 85-90% RH the most severe appeared to be bull's eye rot (Pezicula spp.. The most sensitive cultivars to this disease were: Topaz, Freedom, Goldstar, the least sensitive were Rubinola, Enterprise, Rajka. Other postharvest diseases like gray mold (Botrytis cinerea, blue mold (Penicillium expansum and brown rot (Monilinia fructigena were not common. 'Rajka' and 'Goldstar' were susceptible to bitter pit, and 'Freedom' to superficial scald.

  5. APPLE - An aeroelastic analysis system for turbomachines and propfans

    Science.gov (United States)

    Reddy, T. S. R.; Bakhle, Milind A.; Srivastava, R.; Mehmed, Oral

    1992-01-01

    This paper reviews aeroelastic analysis methods for propulsion elements (advanced propellers, compressors and turbines) being developed and used at NASA Lewis Research Center. These aeroelastic models include both structural and aerodynamic components. The structural models include the typical section model, the beam model with and without disk flexibility, and the finite element blade model with plate bending elements. The aerodynamic models are based on the solution of equations ranging from the two-dimensional linear potential equation for a cascade to the three-dimensional Euler equations for multi-blade configurations. Typical results are presented for each aeroelastic model. Suggestions for further research are indicated. All the available aeroelastic models and analysis methods are being incorporated into a unified computer program named APPLE (Aeroelasticity Program for Propulsion at LEwis).

  6. First evaluation of apple mutants induced by gamma ray treatments

    International Nuclear Information System (INIS)

    In 1972 and 1973 in cooperation with ENEA (Casaccia-Roma) 2758 dormant buds of nine apple varieties (Early Red Stayman, Jerseymac, Jonathan sel. 2 Cooper, Missouri A2071, Mollie's Delicious, Neipling Early Stayman, Ozark Gold, Smoothee, Vistabella) were irradiated with gamma rays 60Co 5–6kR. Mutants were selected in the second vegetative propagation (V2) of the irradiated buds. The most interesting mutants are: 6 for compact tree growth (4 of Mollie's Delicious, 1 of Early Red Stayman, and 1 of Missuori A2071) and 1 for high skin color (Neipling Early Stayman). Seven out of twelve mutants of Mollie's Delicious evaluated for red skin color resulted to be more colored than the control. (author)

  7. Gamma and MNNG mutants for bioconversion of apple distillery waste

    International Nuclear Information System (INIS)

    The mutation of Aspergillus niger by gamma irradiation and MNNG treatment for gaining hyperenzyme producing strains was continued. Potentially useful mutants were evaluated in fermentation tests for enhanced hydrolytic activities and also improved technological properties e.g. improved filtration. Enhanced cellulase activities (endoglucanase, FP activity, aryl-beta-glucosidase) were found. Amylolytic and pectolytic activities also increased. Trichoderma sp. was mutated by gamma rays and MNNG. Mutants were screened for their cellulolytic activities and more active mutants tested in fermentation tests. The selected A. niger and Trichoderma sp. mutants were used together in mixed culture and certain improvements of the bioconversion of apple distillery waste gained. (author). 17 refs, 3 figs, 6 tabs

  8. Concept of integral protection of apple and pear

    Directory of Open Access Journals (Sweden)

    Veličković Milovan M.

    2015-01-01

    Full Text Available This paper implies current and economically significant diseases and pests of an apple and pear, i.e. problems of integral protection of these species of fruits in our country. Measures for their prevention are recommended regarding an adequate period. Special consideration is given to the diseases such as Venturia pyrina, Venturia inaequalis, Monilinia fructigena, Podosphaera leucotricha and Chondrostereum purpureum, as well as to pests such as Cydia pomonella, Cacopsylla pyri, Cacopsilla pyrisuga, Eryiophidae, Panonychus ulmi and Aphididae. The demand and importance of mass implementation of the concept of integral production in terms of obtaining biologically more valuable and ecologically safer fruits, i.e. preservation of the environment and health have been implied.

  9. Secondary Metabolites from Three Florida Sponges with Antidepressant Activity

    OpenAIRE

    Kochanowska, Anna J.; Rao, Karumanchi V.; Childress, Suzanne; El-Alfy, Abir; Matsumoto, Rae R.; Kelly, Michelle; Stewart, Gina S.; Sufka, Kenneth J.; Mark T. Hamann

    2008-01-01

    Brominated indole alkaloids are a common class of metabolites reported from sponges of the order Verongida. Herein we report the isolation, structure determination, and activity of metabolites from three Florida sponges, namely, Verongula rigida (order Verongida, family Aplysinidae), Smenospongia aurea, and S. cerebriformis (order Dictyoceratida, family Thorectidae). All three species were investigated chemically, revealing similarities in secondary metabolites. Brominated compounds, as well ...

  10. SECONDARY METABOLITES OF OCIMUM GRATISSIMUM AND THEIR BIOLOGICAL ACTIVITIES

    OpenAIRE

    Deeptanjali Sahoo; Ajay Kumar

    2013-01-01

    Ocimum gratissimum synthesizes and accumulates a variety of secondary metabolites. Some of the biologically active secondary metabolites such as eugenol, thymol, methyl cinnamate and geraniol are responsible for the antimicrobial activity of the well-known plant of this species and substantiate the claim in traditional system of medicine. The present review summarizes the information available on the secondary metabolites isolated from Ocimum gratissimum.

  11. Antibacterial Secondary Metabolites from the Cave Sponge Xestospongia sp

    OpenAIRE

    Sridevi Ankisetty; Marc Slattery

    2012-01-01

    Chemical investigation of the cave sponge Xestospongia sp. resulted in the isolation of three new polyacetylenic long chain compounds along with two known metabolites. The structures of the new metabolites were established by NMR and MS analyses. The antibacterial activity of the new metabolites was also evaluated.

  12. 40 CFR 159.179 - Metabolites, degradates, contaminants, and impurities.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Metabolites, degradates, contaminants.../Benefit Information § 159.179 Metabolites, degradates, contaminants, and impurities. (a) Metabolites and... degradation of less than 10 percent in a 30-day period. (b) Contaminants and impurities. The presence in...

  13. Management strategies in apple orchards influence earwig community.

    Science.gov (United States)

    Malagnoux, Laure; Marliac, Gaëlle; Simon, Sylvaine; Rault, Magali; Capowiez, Yvan

    2015-04-01

    Our aim was to assess whether different apple orchard management strategies (low-input, organic, Integrated Pest Management (IPM)) would have an effect on earwigs, which are important natural enemies of apple pests. These commercial orchards were as well compared to abandoned orchards. The density of Forficula auricularia and Forficula pubescens was studied for three years in 74 orchards around Avignon. The pesticide usage, some orchard characteristics and two small-scale landscape parameters were characterized. Pesticide use was significantly different between low-input, organic and IPM orchards with particularly significant differences in the number of insecticide applications (2.2, 4.9 and 9.2 respectively). Pesticide use had a much stronger impact on earwig community than other characteristics. F. auricularia density was significantly lower in IPM orchards (0.47 individuals per tree) compared to organic, low-input and abandoned orchards (3.1, 4.5 and 1.6 individuals per tree, respectively). F. pubescens was almost absent from IPM orchards and its abundance was higher in abandoned or low-input orchards compared to organic orchards (1.5 and 2.8 vs 0.8 individuals per tree). The percentage of F. pubescens in the earwig community decreased from abandoned (52%) to low-input (40%), organic (15%) and IPM orchards (0.5%). These results were confirmed by LD50 assays showing that for the two pesticides causing mortality close to normal application rates (chlorpyrifos-ethyl and acetamiprid), F. pubescens was significantly more sensitive than F. auricularia. Since earwigs are also easy to capture and identify, they may be useful to estimate the effects of management strategies and their modification in pome fruit orchards. PMID:25577700

  14. Apple hypanthium firmness: New insights from comparative proteomics

    KAUST Repository

    Marondedze, Claudius

    2012-06-26

    Fruit firmness constitutes an important textural property and is one of the key parameters for estimating ripening and shelf life, which has a major impact on commercialization. In order to decipher the mechanisms related to firmness of apples (Malus × domestica Borkh.), two-dimensional gel electrophoresis (2-DE) was used to compare the total proteome of high and low firmness phenotypes from apple hypanthia of a \\'Golden Delicious\\' × \\'Dietrich\\' population. A total of 36 differentially regulated protein spots were positively identified by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and then validated against the Malus expressed sequence tags (EST) database. The findings of this study indicated a lower expression of ethylene biosynthesis related proteins in the high firmness phenotype, which could be linked to the slowing down of the ripening and softening processes. The reduced accumulation of proteins involved in ethylene biosynthesis juxtaposed to the upregulation of a transposase and a GTP-binding protein in the high firmness phenotype. The results also showed higher expression of cytoskeleton proteins in the high firmness phenotype compared to the low firmness phenotype, which play a role in maintaining cell structure and possibly fruit integrity. Finally, a number of proteins involved in detoxification and defense were expressed in fruit hypanthium. This proteomic study provides a contribution towards a better understanding of regulatory networks involved in fruit hypanthium firmness and/or softening, which could be instrumental in the development of improved fruit quality. © 2012 Springer Science+Business Media, LLC.

  15. Lysimeter experiments on root uptake of Co-60, Sr-90 and Cs-137 from soil into vine and apple trees and on the transfer into grapes and apples

    International Nuclear Information System (INIS)

    In lysimeters filled with two different soil types (Parabraunerde and Podzol) the transfer of 60Co, 90Sr and 137Cs from soil into vine and apple trees was investigated over a time period of 5 years (1988-1992). The soil was contaminated in 1978, so that at the beginning of the experiment the radionuclides were already aged. Due to the low availability for root uptake, the transfer of 60Co and 137Cs into vine and apple trees was very low. 90Sr was fairly available for root uptake which caused a considerable uptake and translocation into vegetative plant parts. The physiological behaviour of the radionuclides investigated determined generally a low transfer into must and apples. This was confirmed by the transfer factors variing between 0.001 and 0.029 for 60Co, 0.01 and 0.036 for 90Sr and 0.001 and 0.109 for 137Cs, respectively. The corresponding values in apples were in the same order of magnitude. The influence of the soil type is shown by the higher incorporation of 60Co, 90Sr and 137Cs into the single plant organs and by the higher transfer factors in must and apples grown on the podzolic soil. (orig.)

  16. Results of isotopic investigations of nitrogen nutrition of apples and cherries

    International Nuclear Information System (INIS)

    Results are reported of 15N study of absorption and dislocation of nitrogen fertilization in apple and cherry plantations and field trials localized nitrogen fertilization of apple plantations. It is found that there is a good functional relation between the individual roots and all skeletal branches of the crown. The apple- and cherry trees can satisfy their nitrogen needs by a part of their root system and hence the introduction of nitrogen into the whole nutrition area is not absolutely necessary. The localized nitrogen fertilization in every other row or only in the row band of apple plantations increases its biological, economic and ecological efficiency. The method is patented. 2 tabs, 7 figs, 5 refs

  17. Application of half-embryo test to irradiated apples and cherries

    International Nuclear Information System (INIS)

    The half-embryo test was applied to irradiated apples and cherries. The optimum incubation temperature for apples and cherries was 30oC and 25oC, respectively. Benzyladenine stimulated the shooting of cherry half-embryos, therefore, they were incubated with 10 μM benzyladenine. The irradiation of apples and cherries caused obvious changes in the growth of the half-embryos. A dose of 0.15 kGy or more almost totally retarded shoot elongation. If shooting is less than 50%, the apples and cherries are identified as ''irradiated''. An assessment could be made after 1 to 4 days and the detection limit of the irradiation dose is 0.15 kGy. (author)

  18. Diversity of soil macrofauna (Diplopoda, Chilopoda and Oniscidea) in apple orchards under different production systems

    Czech Academy of Sciences Publication Activity Database

    Tajovský, Karel

    Coimbra : University of Coimbra, 2012. s. 53. [International Colloquium on Soil Zoology /16./. 06.08.2012-10.08.2012, Coimbra] Institutional support: RVO:60077344 Keywords : diversity * soil macrofauna * apple orchards Subject RIV: EH - Ecology, Behaviour

  19. Life history studies and habitat requirements of the apple snail at Lake Woodruff National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This study discusses habitat requirements and life history of the apple snail at Lake Woodruff National Wildlife Refuge. A study was initiated during 1971 to gather...

  20. The effect of ultraviolet irradiation on shelf-life and ripening of peaches and apples

    International Nuclear Information System (INIS)

    ''Loring'' and ''Elberta'' peaches and ''Golden Delicious'' apples were irradiated with UV (254nm) to doses of 0.84 X 10(4) to 40 X 10(4) erg/mm2 then stored. ''Loring'' were stored 10 days and ''Elberta'' 20 days at 12 degrees C. ''Golden Delicious'' were stored 30 days at 20-25 degrees C in a dark room. Fruit were examined and differences in percentage rot and in physical-chemical properties determined. Percentage rot decreased with increasing UV dose. Fruit were firmer, pH and soluble solids lower and acidity higher for UV-treated than for nontreated peaches; pH was lower and acidity and ascorbic acid higher in UV treated than in nontreated apples. Percentage weight loss was less for UV-treated apples. The results indicated that UV treatment not only reduced storage rots but also delayed ripening of peaches and apples