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Sample records for appendix g1 papers

  1. Control and monitor instrumentation for the 400 kV heavy ion accelerator at IGCAR Kalpakkam [Paper No.:G1

    International Nuclear Information System (INIS)

    A multichannel remote control fibre optic telemetry system has been developed and installed for the 400 kV Heavy Ion Accelerator at Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam, to control and monitor different parameters of the subsystems of the accelerator, housed at the high voltage terminal. Such a system is necessary in this case, since it is not possible to take the signals through electrical leads from the 400 kV terminal to the control console at the ground potential. Signals have to be transmitted through a proper insulating medium. A fibre optic telemetry system is chosen for this purpose. The telemetry system works on the following principle: The analog electrical signal is converted into digital light signal and transmitted through a light pipe. At the other end of the light pipe, the light signal is converted back into electrical analog signal. The control and monitoring signals are transmitted from the control console at ground potential to the equipment located at the high voltage terminal and vice versa using the optical telemetry system. The circuit description and operation details of the multichannel fibre optic telemetry system are presented in this paper. (author). 7 figs., 1 tab

  2. Papers

    DEFF Research Database (Denmark)

    Thoft-Christensen, Palle

    The chapters in this Volume 7 of a series of PAPERS are based on papers published in the period 2004 - 2008 authored/co-authored by Palle Thoft-Christensen......The chapters in this Volume 7 of a series of PAPERS are based on papers published in the period 2004 - 2008 authored/co-authored by Palle Thoft-Christensen...

  3. G1 Continuity Conditions of B-spline Surfaces

    Institute of Scientific and Technical Information of China (English)

    车翔玖; 梁学章

    2002-01-01

    According to the B-spline theory and Boehm algorithm,this paper presents several necessary and sufficient G1 continuity conditions betwwen two adjacent B-spline surfaces,In Order to meet the need of application,a kind of sufficient conditions of G1 continuity are developed,and a kind of sufficient conditions of G1 continuity among N(N>2) patch B-shline surfaces meeting at a common corner are given at the end.

  4. Acute appendicitis with a neuroendocrine tumor G1 (carcinoid): pitfalls of conservative treatment.

    Science.gov (United States)

    Watanabe, Hiroyuki A; Fujimoto, Taketoshi; Kato, Yo; Sasaki, Mayumi; Ikusue, Toshikazu

    2016-08-01

    A man in his early thirties presented to our clinic with right lower abdominal pain. Computed tomography (CT) and ultrasonography (US) revealed a swollen appendix and an appendicolith. Abscess formation was not observed but ongoing appendiceal rupture was not ruled out. Three months after successful conservative therapy, the lumen of the apical portion was kept dilated and laparoscopic interval appendectomy was performed. No tumorous findings were observed macroscopically. However, histology revealed many tiny nests infiltrating the submucosa, muscular layer, and subserosa at the root of the appendix. An appendiceal neuroendocrine tumor G1 (NET G1; carcinoid) was diagnosed immunohistologically. Neither CT nor US visualized the tumor because of its non-tumor-forming but infiltrative growth. In conclusion, after successful conservative treatment, interval appendectomy should be considered to uncover a possible appendiceal NET G1 (carcinoid), particularly when dilatation of the distal lumen is kept under observation. PMID:27311320

  5. 12 CFR 563g.1 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... same as in Commission Rule 501(a) (17 CFR 230.501(a)) under the Securities Act, and includes any... Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY SECURITIES OFFERINGS § 563g.1 Definitions..., Business Transactions Division, Office of Thrift Supervision, 1700 G Street, NW., Washington, DC...

  6. Identification of waiting time distribution of M/G/1, Mx/G/1, GIr/M/1 queueing systems

    Directory of Open Access Journals (Sweden)

    A. Ghosal

    1988-01-01

    Full Text Available This paper brings out relations among the moments of various orders of the waiting time of the 1st customer and a randomly selected customer of an arrival group for bulk arrivals queueing models, and as well as moments of the waiting time (in queue for M/G/1 queueing system. A numerical study of these relations has been developed in order to find the (β1,β2 measures of waiting time distribution in a comutable form. On the basis of these measures one can look into the nature of waiting time distribution of bulk arrival queues and the single server M/G/1 queue.

  7. G1 Continuity Conditions of B-spline Surfaces%B样条曲面间的G1连续条件

    Institute of Scientific and Technical Information of China (English)

    车翔玖; 梁学章

    2002-01-01

    According to the B-spline theory and Boehm algorithm, this paper presents severalnecessary and sufficient G1 continuity conditions between two adjacent B-spline surfaces. In orderto meet the need of application, a kind of sufficient conditions of G1 continuity are developed, anda kind of sufficient conditions of G1 continuity among N(N > 2) patch B-spline surfaces meetingat a common corner are given at the end.

  8. Trisomía g1 (mongolismo)

    OpenAIRE

    Yunis, Emilio; Federicci de la Cruz, Emilia; Salamanca, Fabio; Abuabara, Sabas

    2011-01-01

    La trisomía G1 conocida más comúnmente como Síndrome de Down y Mongolismo fue la primera aberración cromosómica numérica descrita en el humano, en 1959 por Lejeune y Col. Quizás quien primero llamó la atención sobre esta entidad fue Esquirol en 1838, refiriéndose a los pacientes afectados como "esos sujetos cuya talla es pequeña, la cabeza poco voluminosa, la comisura palpebral externa más elevada que la interna, la nariz deprimida en su base". Posteriormente, en 1846, F. Según agregó a la an...

  9. 26 CFR 1.149(g)-1 - Hedge bonds.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Hedge bonds. 1.149(g)-1 Section 1.149(g)-1...) INCOME TAXES (CONTINUED) Tax Exemption Requirements for State and Local Bonds § 1.149(g)-1 Hedge bonds... of replacement proceeds (other than amounts in a bona fide debt service fund or a reasonably...

  10. Rapid biodegradation of organophosphorus pesticides by Stenotrophomonas sp. G1

    Energy Technology Data Exchange (ETDEWEB)

    Deng, Shuyan; Chen, Yao [Key Laboratory of Agri-food Safety of Anhui Province, Lab of Quality & Safety and Risk Assessment for Agro-products on Storage and Preservation (Hefei), Ministry of Agriculture, School of Resource and Environment, Anhui Agricultural University, Hefei 230036 (China); Wang, Daosheng [School of Life Science, Anhui Agricultural University, Hefei 230036 (China); Shi, Taozhong; Wu, Xiangwei; Ma, Xin; Li, Xiangqiong [Key Laboratory of Agri-food Safety of Anhui Province, Lab of Quality & Safety and Risk Assessment for Agro-products on Storage and Preservation (Hefei), Ministry of Agriculture, School of Resource and Environment, Anhui Agricultural University, Hefei 230036 (China); Hua, Rimao, E-mail: rimaohua@ahau.edu.cn [Key Laboratory of Agri-food Safety of Anhui Province, Lab of Quality & Safety and Risk Assessment for Agro-products on Storage and Preservation (Hefei), Ministry of Agriculture, School of Resource and Environment, Anhui Agricultural University, Hefei 230036 (China); Tang, Xinyun [School of Life Science, Anhui Agricultural University, Hefei 230036 (China); Li, Qing X. [Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East–West Road, Honolulu, HI 957822 (United States)

    2015-10-30

    Highlights: • Stenotrophomonas sp. G1 was isolated from chlorpyrifos contaminated sludge. • Strain G1 is closest to Stenotrophomonas acidaminiphila. • Strain G1 can efficiently degrade 8 organophosphorus pesticides (OPs). • Intracellular methyl parathion hydrolase is responsible for the OP degradation. • Three factors were orthogonally optimized for degradation of methyl parathion. - Abstract: Organophosphorus insecticides have been widely used, which are highly poisonous and cause serious concerns over food safety and environmental pollution. A bacterial strain being capable of degrading O,O-dialkyl phosphorothioate and O,O-dialkyl phosphate insecticides, designated as G1, was isolated from sludge collected at the drain outlet of a chlorpyrifos manufacture plant. Physiological and biochemical characteristics and 16S rDNA gene sequence analysis suggested that strain G1 belongs to the genus Stenotrophomonas. At an initial concentration of 50 mg/L, strain G1 degraded 100% of methyl parathion, methyl paraoxon, diazinon, and phoxim, 95% of parathion, 63% of chlorpyrifos, 38% of profenofos, and 34% of triazophos in 24 h. Orthogonal experiments showed that the optimum conditions were an inoculum volume of 20% (v/v), a substrate concentration of 50 mg/L, and an incubation temperature in 40 °C. p-Nitrophenol was detected as the metabolite of methyl parathion, for which intracellular methyl parathion hydrolase was responsible. Strain G1 can efficiently degrade eight organophosphorus pesticides (OPs) and is a very excellent candidate for applications in OP pollution remediation.

  11. Rapid biodegradation of organophosphorus pesticides by Stenotrophomonas sp. G1

    International Nuclear Information System (INIS)

    Highlights: • Stenotrophomonas sp. G1 was isolated from chlorpyrifos contaminated sludge. • Strain G1 is closest to Stenotrophomonas acidaminiphila. • Strain G1 can efficiently degrade 8 organophosphorus pesticides (OPs). • Intracellular methyl parathion hydrolase is responsible for the OP degradation. • Three factors were orthogonally optimized for degradation of methyl parathion. - Abstract: Organophosphorus insecticides have been widely used, which are highly poisonous and cause serious concerns over food safety and environmental pollution. A bacterial strain being capable of degrading O,O-dialkyl phosphorothioate and O,O-dialkyl phosphate insecticides, designated as G1, was isolated from sludge collected at the drain outlet of a chlorpyrifos manufacture plant. Physiological and biochemical characteristics and 16S rDNA gene sequence analysis suggested that strain G1 belongs to the genus Stenotrophomonas. At an initial concentration of 50 mg/L, strain G1 degraded 100% of methyl parathion, methyl paraoxon, diazinon, and phoxim, 95% of parathion, 63% of chlorpyrifos, 38% of profenofos, and 34% of triazophos in 24 h. Orthogonal experiments showed that the optimum conditions were an inoculum volume of 20% (v/v), a substrate concentration of 50 mg/L, and an incubation temperature in 40 °C. p-Nitrophenol was detected as the metabolite of methyl parathion, for which intracellular methyl parathion hydrolase was responsible. Strain G1 can efficiently degrade eight organophosphorus pesticides (OPs) and is a very excellent candidate for applications in OP pollution remediation

  12. HERMES Precision Results on g1p, g1d and g1n and the First Measurement of the Tensor Structure Function b1d

    CERN Document Server

    Riedl, C; Akopov, Z; Amarian, M; Ammosov, V V; Andrus, A; Aschenauer, E C; Augustyniak, W; Avakian, R; Avetisian, A; Avetissian, E; Bailey, P; Baturin, V; Baumgarten, C; Beckmann, M; Belostotskii, S; Bernreuther, S; Bianchi, N; Blok, H P; Böttcher, Helmut B; Borisov, A; Bouwhuis, M; Brack, J; Brüll, A; Bryzgalov, V V; Capitani, G P; Chiang, H C; Ciullo, G; Contalbrigo, M; Dalpiaz, P F; De Leo, R; De Nardo, L; De Sanctis, E; Devitsin, E G; Di Nezza, P; Düren, M; Ehrenfried, M; Elalaoui-Moulay, A; Elbakian, G M; Ellinghaus, F; Elschenbroich, U; Ely, J; Fabbri, R; Fantoni, A; Feshchenko, A; Felawka, L; Fox, B; Franz, J; Frullani, S; Gärber, Y; Gapienko, G; Gapienko, V; Garibaldi, F; Garrow, K; Garutti, E; Gaskell, D; Gavrilov, G E; Karibian, V; Graw, G; Grebenyuk, O; Greeniaus, L G; Hafidi, K; Hartig, M; Hasch, D; Heesbeen, D; Henoch, M; Hertenberger, R; Hesselink, W H A; Hillenbrand, A; Hoek, M; Holler, Y; Hommez, B; Iarygin, G; Ivanilov, A; Izotov, A; Jackson, H E; Jgoun, A; Kaiser, R; Kinney, E; Kiselev, A; Königsmann, K C; Kopytin, M; Korotkov, V A; Kozlov, V; Krauss, B; Krivokhizhin, V G; Lagamba, L; Lapikas, L; Laziev, A; Lenisa, P; Liebing, P; Lindemann, T; Lipka, K; Lorenzon, W; Lü, J; Maiheu, B; Makins, N C R; Marianski, B; Marukyan, H O; Masoli, F; Mexner, V; Meyners, N; Miklukho, O; Miller, C A; Miyachi, Y; Muccifora, V; Nagaitsev, A; Nappi, E; Naryshkin, Yu; Nass, A; Negodaev, M A; Nowak, Wolf-Dieter; Oganessyan, K; Ohsuga, H; Orlandi, G; Pickert, N; Potashov, S Yu; Potterveld, D H; Raithel, M; Reggiani, D; Reimer, P E; Reischl, A; Reolon, A R; Rith, K; Airapetian, A; Rosner, G; Rostomyan, A; Rubacek, L; Ryckbosch, D; Salomatin, Yu I; Sanjiev, I; Savin, I; Scarlett, C; Schäfer, A; Schill, C; Schnell, G; Schüler, K P; Schwind, A; Seele, J; Seidl, R; Seitz, B; Shanidze, R G; Shearer, C; Shibata, T A; Shutov, V B; Simani, M C; Sinram, K; Stancari, M D; Statera, M; Steffens, E; Steijger, J J M; Stewart, J; Stösslein, U; Tait, P; Tanaka, H; Taroian, S P; Tchuiko, B; Terkulov, A R; Tkabladze, A V; Trzcinski, A; Tytgat, M; Vandenbroucke, A; Van der Nat, P B; van der Steenhoven, G; Vetterli, Martin C; Vikhrov, V; Vincter, M G; Visser, J; Vogel, C; Vogt, M; Volmer, J; Weiskopf, C; Wendland, J; Wilbert, J; Ybeles-Smit, G V; Yen, S; Zihlmann, B; Zohrabyan, H G; Zupranski, P; Riedl, Caroline

    2005-01-01

    Final HERMES results on the proton, deuteron and neutron structure function g1 are presented in the kinematic range 0.0021

  13. Tolerance of Deregulated G1/S Transcription Depends on Critical G1/S Regulon Genes to Prevent Catastrophic Genome Instability

    Directory of Open Access Journals (Sweden)

    Catia Caetano

    2014-12-01

    Full Text Available Expression of a G1/S regulon of genes that are required for DNA replication is a ubiquitous mechanism for controlling cell proliferation; moreover, the pathological deregulated expression of E2F-regulated G1/S genes is found in every type of cancer. Cellular tolerance of deregulated G1/S transcription is surprising because this regulon includes many dosage-sensitive proteins. Here, we used the fission yeast Schizosaccharomyces pombe to investigate this issue. We report that deregulating the MBF G1/S regulon by eliminating the Nrm1 corepressor increases replication errors. Homology-directed repair proteins, including MBF-regulated Ctp1CtIP, are essential to prevent catastrophic genome instability. Surprisingly, the normally inconsequential MBF-regulated S-phase cyclin Cig2 also becomes essential in the absence of Nrm1. This requirement was traced to cyclin-dependent kinase inhibition of the MBF-regulated Cdc18Cdc6 replication origin-licensing factor. Collectively, these results establish that, although deregulation of G1/S transcription is well tolerated by cells, nonessential G1/S target genes become crucial for preventing catastrophic genome instability.

  14. Strategic cell-cycle regulatory features that provide mammalian cells with tunable G1 length and reversible G1 arrest.

    Directory of Open Access Journals (Sweden)

    Benjamin Pfeuty

    Full Text Available Transitions between consecutive phases of the eukaryotic cell cycle are driven by the catalytic activity of selected sets of cyclin-dependent kinases (Cdks. Yet, their occurrence and precise timing is tightly scheduled by a variety of means including Cdk association with inhibitory/adaptor proteins (CKIs. Here we focus on the regulation of G1-phase duration by the end of which cells of multicelled organisms must decide whether to enter S phase or halt, and eventually then, differentiate, senesce or die to obey the homeostatic rules of their host. In mammalian cells, entry in and progression through G1 phase involve sequential phosphorylation and inactivation of the retinoblastoma Rb proteins, first, by cyclin D-Cdk4,6 with the help of CKIs of the Cip/Kip family and, next, by the cyclin E-Cdk2 complexes that are negatively regulated by Cip/Kip proteins. Using a dynamical modeling approach, we show that the very way how the Rb and Cip/Kip regulatory modules interact differentially with cyclin D-Cdk4,6 and cyclin E-Cdk2 provides to mammalian cells a powerful means to achieve an exquisitely-sensitive control of G1-phase duration and fully reversible G1 arrests. Consistently, corruption of either one of these two modules precludes G1 phase elongation and is able to convert G1 arrests from reversible to irreversible. This study unveils fundamental design principles of mammalian G1-phase regulation that are likely to confer to mammalian cells the ability to faithfully control the occurrence and timing of their division process in various conditions.

  15. 26 CFR 1.514(g)-1 - Business lease indebtedness.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Business lease indebtedness. 1.514(g)-1 Section 1... Business lease indebtedness. (a) Definition. The term business lease indebtedness means, with respect to... subsidiary corporations. (b) Examples. The rules of section 514(g) respecting business leases also...

  16. 26 CFR 1.167(g)-1 - Basis for depreciation.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Basis for depreciation. 1.167(g)-1 Section 1.167... for depreciation. The basis upon which the allowance for depreciation is to be computed with respect... property at that time, is the basis for computing depreciation....

  17. Rapid biodegradation of organophosphorus pesticides by Stenotrophomonas sp. G1.

    Science.gov (United States)

    Deng, Shuyan; Chen, Yao; Wang, Daosheng; Shi, Taozhong; Wu, Xiangwei; Ma, Xin; Li, Xiangqiong; Hua, Rimao; Tang, Xinyun; Li, Qing X

    2015-10-30

    Organophosphorus insecticides have been widely used, which are highly poisonous and cause serious concerns over food safety and environmental pollution. A bacterial strain being capable of degrading O,O-dialkyl phosphorothioate and O,O-dialkyl phosphate insecticides, designated as G1, was isolated from sludge collected at the drain outlet of a chlorpyrifos manufacture plant. Physiological and biochemical characteristics and 16S rDNA gene sequence analysis suggested that strain G1 belongs to the genus Stenotrophomonas. At an initial concentration of 50 mg/L, strain G1 degraded 100% of methyl parathion, methyl paraoxon, diazinon, and phoxim, 95% of parathion, 63% of chlorpyrifos, 38% of profenofos, and 34% of triazophos in 24 h. Orthogonal experiments showed that the optimum conditions were an inoculum volume of 20% (v/v), a substrate concentration of 50 mg/L, and an incubation temperature in 40 °C. p-Nitrophenol was detected as the metabolite of methyl parathion, for which intracellular methyl parathion hydrolase was responsible. Strain G1 can efficiently degrade eight organophosphorus pesticides (OPs) and is a very excellent candidate for applications in OP pollution remediation.

  18. Nonparametric estimation of the stationary M/G/1 workload distribution function

    DEFF Research Database (Denmark)

    Hansen, Martin Bøgsted

    2005-01-01

    In this paper it is demonstrated how a nonparametric estimator of the stationary workload distribution function of the M/G/1-queue can be obtained by systematic sampling the workload process. Weak convergence results and bootstrap methods for empirical distribution functions for stationary associ...

  19. ON THE TRANSIENT DEPARTURE PROCESS OF Mx/G/1 QUEUEING SYSTEM WITH SINGLE SERVER VACATION

    Institute of Scientific and Technical Information of China (English)

    Yinghui TANG

    2007-01-01

    This paper studies the transient departure process of Mx/G/1 queueing system with single server vacation. We present a simple probability decomposition method to derive the expected number of departures occurring in finite time interval from any initial state and the asymptotic expansion of the expected number. Especially, we derive some more practical results for some special cases.

  20. Brill-Noether locus of rank 1 and degree g-1 on a nodal curve

    CERN Document Server

    Coelho, Juliana

    2011-01-01

    In this paper we consider the Brill-Noether locus $W_{\\underline d}(C)$ of line bundles of multidegree $\\underline d$ of total degree $g-1$ having a nonzero section on a nodal reducible curve $C$ of genus $g\\geq2$. We give an explicit description of the irreducible components of $W_{\\underline d}(C)$ for a semistable multidegre $\\underline d$. As a consequence we show that, if two semistable multidegrees of total degre $g-1$ on a curve with no rational components differ by a twister, then the respective Brill-Noether loci have isomorphic components.

  1. Design principles of the yeast G1/S switch.

    Directory of Open Access Journals (Sweden)

    Xiaojing Yang

    2013-10-01

    Full Text Available A hallmark of the G1/S transition in budding yeast cell cycle is the proteolytic degradation of the B-type cyclin-Cdk stoichiometric inhibitor Sic1. Deleting SIC1 or altering Sic1 degradation dynamics increases genomic instability. Certain key facts about the parts of the G1/S circuitry are established: phosphorylation of Sic1 on multiple sites is necessary for its destruction, and both the upstream kinase Cln1/2-Cdk1 and the downstream kinase Clb5/6-Cdk1 can phosphorylate Sic1 in vitro with varied specificity, cooperativity, and processivity. However, how the system works as a whole is still controversial due to discrepancies between in vitro, in vivo, and theoretical studies. Here, by monitoring Sic1 destruction in real time in individual cells under various perturbations to the system, we provide a clear picture of how the circuitry functions as a switch in vivo. We show that Cln1/2-Cdk1 sets the proper timing of Sic1 destruction, but does not contribute to its destruction speed; thus, it acts only as a trigger. Sic1's inhibition target Clb5/6-Cdk1 controls the speed of Sic1 destruction through a double-negative feedback loop, ensuring a robust all-or-none transition for Clb5/6-Cdk1 activity. Furthermore, we demonstrate that the degradation of a single-phosphosite mutant of Sic1 is rapid and switch-like, just as the wild-type form. Our mathematical model confirms our understanding of the circuit and demonstrates that the substrate sharing between the two kinases is not a redundancy but a part of the design to overcome the trade-off between the timing and sharpness of Sic1 degradation. Our study provides direct mechanistic insight into the design features underlying the yeast G1/S switch.

  2. Appendix A

    DEFF Research Database (Denmark)

    Henriksen, M. S.; Brincker, Rune; Heshe, Gert

    1999-01-01

    In this appendix a brief summary of experiments on reinforced concrete beams in three-point bending performed at Aalborg University is given. The aim of the investigation is to determine the full load-deflection curves for different beam sizes, different types of concrete and different amounts an...

  3. Appendix C

    DEFF Research Database (Denmark)

    Christensen, F. A.; Henriksen, M. S.; Brincker, Rune

    1999-01-01

    In this appendix a model is formulated for the rotational capacity of reinforced concrete beams assuming rebar tension failure. The model is based on a classical approach and establishes the load-deflection curve of a reinforced concrete beam. The rotational capacity is then obtained as the area ...

  4. Appendix B

    DEFF Research Database (Denmark)

    Christensen, F. A.; Brincker, Rune

    1999-01-01

    In this appendix the failure behaviour of lightly reinforced concrete beams is investigated. A numerical model based on the fictitious crack approach according to Hillerborg [1] is established in order to estimate the load-deflection curve for lightly reinforced concrete beams. The debonding betw...

  5. Early evolution of disrupted asteroid P/2016 G1 (PANSTARRS)

    CERN Document Server

    Moreno, Fernando; Cabrera-Lavers, Antonio; Pozuelos, Francisco J

    2016-01-01

    We present deep imaging observations of activated asteroid P/2016 G1 (PANSTARRS) using the 10.4m Gran Telescopio Canarias (GTC) from late April to early June 2016. The images are best interpreted as the result of a relatively short-duration event with onset about $\\mathop{350}_{-30}^{+10}$ days before perihelion (i.e., around 10th February, 2016), starting sharply and decreasing with a $\\mathop{24}_{-7}^{+10}$ days (Half-width at half-maximum, HWHM). The results of the modeling imply the emission of $\\sim$1.7$\\times$10$^7$ kg of dust, if composed of particles of 1 micrometer to 1 cm in radius, distributed following a power-law of index --3, and having a geometric albedo of 0.15. A detailed fitting of a conspicuous westward feature in the head of the comet-like object indicates that a significant fraction of the dust was ejected along a privileged direction right at the beginning of the event, which suggests that the parent body has possibly suffered an impact followed by a partial or total disruption. From th...

  6. Early Evolution of Disrupted Asteroid P/2016 G1 (PANSTARRS)

    Science.gov (United States)

    Moreno, F.; Licandro, J.; Cabrera-Lavers, A.; Pozuelos, F. J.

    2016-08-01

    We present deep imaging observations of activated asteroid P/2016 G1 (PANSTARRS) using the 10.4 m Gran Telescopio Canarias (GTC) from 2016 late April to early June. The images are best interpreted as the result of a relatively short-duration event with an onset of about {350}-30+10 days before perihelion (i.e., around 2016 February 10), starting sharply and decreasing with {24}-7+10 days (HWHM). The results of the modeling imply that the emission of ˜1.7 × 107 kg of dust, if composed of particles of 1 μm to 1 cm in radius, is distributed following a power law of index ‑3 and having a geometric albedo of 0.15. A detailed fitting of a conspicuous westward feature in the head of the comet-like object indicates that a significant fraction of the dust was ejected along a privileged direction right at the beginning of the event, which suggests that the parent body has possibly suffered an impact followed by a partial or total disruption. From the limiting magnitude reachable with the instrumental setup, and assuming a geometric albedo of 0.15 for the parent body, an upper limit for the size of possible fragment debris of ˜50 m in radius is derived.

  7. Insensitive bounds for the moments of the sojourn time distribution in the M/G/1 processor-sharing queue

    NARCIS (Netherlands)

    Cheung, S.K.; Berg, van den J.L.; Boucherie, R.J.

    2005-01-01

    This paper studies the M/G/1 processor-sharing (PS) queue and the sojourn time distribution conditioned on the initial job size. Although several expressions for the Laplace-Stieltjes transform (LST) are known, these expressions are not applicable for computational purposes. This paper derives readi

  8. Insensitive bounds for the moments of the sojourn time distribution in the M/G/1 processor-sharing queue

    NARCIS (Netherlands)

    Cheung, S.K.; Berg, J.L. van den; Boucherie, R.J.

    2006-01-01

    This paper studies the M/G/1 processor-sharing (PS) queue, in particular the sojourn time distribution conditioned on the initial job size. Although several expressions for the Laplace-Stieltjes transform (LST) are known, these expressions are not suitable for computational purposes. This paper deri

  9. 26 CFR 1.904(g)-1 - Overall domestic loss and the overall domestic loss account.

    Science.gov (United States)

    2010-04-01

    ... loss account. 1.904(g)-1 Section 1.904(g)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF... States § 1.904(g)-1 Overall domestic loss and the overall domestic loss account. For further guidance, see § 1.904(g)-1T....

  10. The kernel G1(x,x') and the quantum equivalence principle

    International Nuclear Information System (INIS)

    In this paper, it is re-examined the formulation of the quantum equivalence principle (QEP) and its compatibility with the conditions which must be fulfilled by the kernel G1(x,x') is discussed. It is also determined the base of solutions which give the particle model in a curved space-time in terms of Cauchy's data for such a kernel. Finally, it is analyzed the creation of particles in this model by studying the time evolution of creation and annihilation operators. This method is an alternative to one that uses Bogoliubov's transformation as a mechanism of creation. (author)

  11. SOME NEW RESULTS ON WAITING TIME AND BUSY TIME IN M/G/1 QUEUE

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    This paper considers an M/G/1 queue with Poisson rate λ > 0 and servicetime distribution G(t) which is supposed to have finite mean 1/μ. The following questions are first studied: (a) The closed bounds of the probability that waiting time is more than a fixed value; (b)The total busy time of the server, which including the distribution,probability that are more than a fixed value during a given time interval (0, t], and the expected value. Some new and important results are obtained by theories of the classes of life distributions and renewal process.

  12. 26 CFR 301.6511(g)-1 - Special rule for partnership items of federally registered partnerships.

    Science.gov (United States)

    2010-04-01

    ... registered partnerships. 301.6511(g)-1 Section 301.6511(g)-1 Internal Revenue INTERNAL REVENUE SERVICE... Limitations on Assessment and Collection § 301.6511(g)-1 Special rule for partnership items of federally...(g) must also be taken into account in applying the various special periods of limitation...

  13. 26 CFR 1.642(g)-1 - Disallowance of double deductions; in general.

    Science.gov (United States)

    2010-04-01

    ....642(g)-1 Section 1.642(g)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Estates, Trusts, and Beneficiaries § 1.642(g)-1 Disallowance..., after a statement is filed under section 642(g) with respect to a particular item or portion of an...

  14. Effect of PKC pathway on G1/S progression control in HeLa cells

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The effect of PKC activity on G1/S progression in HeLa cells has been studied.The result shows that (ⅰ) PKC activity alteration in G1 phase affects G1/S progression in HeLa cells.It has been observed that G1/S progression is stimulated by PKC agonist TPA and inhibited by PKC inhibitor GF-109203X.(ⅱ) The expression of c-myc and c-jun is stimulated by TPA and inhibited by GF-109203X treatment in early G1 phase.(ⅲ) During G1/S progression,the expression of CyclinD1 is stimulated by TPA treatment and inhibited by GF-109203X treatment.There is no effect on the expression of CDK4.It is likely that PKC pathway regulates G1/S progression through regulating the expression of some early response genes and engine molecules in HeLa cells.

  15. Fluid Approximation and Its Convergence Rate for GI/G/1 Queue with Vacations

    Institute of Scientific and Technical Information of China (English)

    Yong-jiang Guo

    2011-01-01

    A GI/G/1 queue with vacations is considered in this paper. We develop an approximating technique on max function of independent and identically distributed (i.i.d.) random variables, that is max{ηi, 1 ≤ i ≤ n}.The approximating technique is used to obtain the fluid approximation for the queue length, workload and busy time processes. Furthermore, under uniform topology, if the scaled arrival process and the scaled service process converge to the corresponding fluid processes with an exponential rate, we prove by the approximating technique that the scaled processes characterizing the queue converge to the corresponding fluid limits with the exponential rate only for large N. Here the scaled processes include the queue length process, workload process and busy time process.

  16. On a BMAP/G/1 G-queue with Setup Times andMultiple Vacations

    Institute of Scientific and Technical Information of China (English)

    Yi PENG; Xiang-qun YANG

    2011-01-01

    In this paper,we consider a BMAP/G/1 G-queue with setup times and multiple vacations.Arrivals of positive customers and negative customers follow a batch Markovian arrival process (BMAP) and Markovian arrival process (MAP) respectively.The arrival of a negative customer removes all the customers in the system when the server is working.The server leaves for a vacation as soon as the system empties and is allowed to take repeated (multiple) vacations.By using the supplementary variables method and the censoring technique,we obtain the queue length distributions.We also obtain the mean of the busy period based on the renewal theory.

  17. TRANSIENT SOLUTION FOR QUEUE-LENGTH DISTRIBUTION OF Geometry/G/1 QUEUEING MODEL

    Institute of Scientific and Technical Information of China (English)

    Luo Chuanyi; Tang Yinghui; Liu Renbin

    2007-01-01

    In this paper, the Geometry/G/1 queueing model with inter-arrival times generated by a geometric(parameter p) distribution according to a late arrival system with delayed access and service times independently distributed with distribution {gj}, j ≥ 1 is studied. By a simple method (techniques of probability decomposition, renewal process theory) that is different from the techniques used by Hunter(1983), the transient property of the queue with initial state i(i ≥ 0) is discussed. The recursion expression for u -transform of transient queue-length distribution at any time point n+ is obtained, and the recursion expression of the limiting queue length distribution is also obtained.

  18. Reliability analysis of M/G/1 queues with general retrial times and server breakdowns

    Institute of Scientific and Technical Information of China (English)

    WANG Jinting

    2006-01-01

    This paper concerns the reliability issues as well as queueing analysis of M/G/1 retrial queues with general retrial times and server subject to breakdowns and repairs. We assume that the server is unreliable and customers who find the server busy or down are queued in the retrial orbit in accordance with a first-come-first-served discipline. Only the customer at the head of the orbit queue is allowed for access to the server. The necessary and sufficient condition for the system to be stable is given. Using a supplementary variable method, we obtain the Laplace-Stieltjes transform of the reliability function of the server and a steady state solution for both queueing and reliability measures of interest. Some main reliability indexes, such as the availability, failure frequency, and the reliability function of the server, are obtained.

  19. TRANSIENT QUEUE SIZE DISTRIBUTION SOLUTION OF GEOM/G/1 QUEUE WITH FEEDBACK-A RECURSIVE METHOD

    Institute of Scientific and Technical Information of China (English)

    Chuanyi LUO; Yinghui TANG; Cailiang LI

    2009-01-01

    This paper considers the Geom/G/1 queueing model with feedback according to a late arrival system with delayed access (LASDA). Using recursive method, this paper studies the transient property of the queue size from the initial state N(0+) = I. Some new results about the recursive expression of the transient queue size distribution at any epoch n+ and the recursive formulae of the equilibrium distribution are obtained, Furthermore, the recursive formulae of the equilibrium queue size distribution at epoch n-, and n are obtained, too. The important relations between stationary queue size distributions at different epochs are discovered (being different from the relations given in M/G/1 queueing system). The model discussed in this paper can be widely applied in all kinds of communications and computer network.

  20. Functional role of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in NSCLC

    Science.gov (United States)

    Cao, Yueyu; Wei, Mengdan; Li, Bing; Liu, Yali; Lu, Ying; Tang, Zhipeng; Lu, Tianbao; Yin, Yujiao; Qin, Zhiqiang; Xu, Zengguang

    2016-01-01

    Eukaryotic translation initiation factor 4 gamma 1(EIF4G1) is related to tumorigenesis and tumor progression. However, its role and the underlying mechanisms in the regulation of tumor development in non–small cell lung cancers (NSCLC) remain largely unknown. Here we report that the levels of EIF4G1 expression are much higher in NSCLC cell lines and tumor tissues than those in the normal lung cells and adjacent normal tissues from the same patients. Using shRNA to knock down EIF4G1 expression stably, we found EIF4G1 required for NSCLC cell proliferation, anchorage-independent growth, migration and invasion. Furthermore, silencing of EIF4G1 induces NSCLC cell apoptosis and causes G0/G1 cell cycle arrest. To identify the partner protein network of EIF4G1 in NSCLC cells, we found that Ubiquitin-specific protease 10 (USP10) can directly interacts with EIF4G1, while acting as a negative regulator for EIF4G1-mediated functions. Together, our results indicate that EIF4G1 functions as an oncoprotein during NSCLC development, which may represent a novel and promising therapeutic target in lung cancer. PMID:27003362

  1. Diversity of VP7 genes of G1 rotaviruses isolated in Iran, 2009-2013.

    Science.gov (United States)

    Jalilvand, Somayeh; Afchangi, Atefeh; Mohajel, Nasir; Roohvand, Farzin; Shoja, Zabihollah

    2016-01-01

    Genotype G1 of rotaviruses (RVs) is the most prevalent strain in human RV infections around the world. The present study evaluated genetic variations in the VP7 gene of RV G1 genotype isolates from Iran. Genetic and phylogenetic analyses indicated that RV strains from Iran clustered with G1 lineages IA, IC, and IIC, showing highest average of similarity versus reference sequences of the G1 lineages I and II. This study highlights the genetic pattern of G1 RV on the basis of distinct lineages and sublineages and indicates the importance of continuous monitoring on genetic variation and evolution pattern of G1 RV strains across the Iranian population for the final aim of RV vaccine introduction.

  2. ACE2 is required for daughter cell-specific G1 delay in Saccharomyces cerevisiae

    OpenAIRE

    Laabs, Tracy L.; Markwardt, David D.; Slattery, Matthew G.; Newcomb, Laura L.; Stillman, David J.; Heideman, Warren

    2003-01-01

    Saccharomyces cerevisiae cells reproduce by budding to yield a mother cell and a smaller daughter cell. Although both mother and daughter begin G1 simultaneously, the mother cell progresses through G1 more rapidly. Daughter cell G1 delay has long been thought to be due to a requirement for attaining a certain critical cell size before passing the commitment point in the cell cycle known as START. We present an alternative model in which the daughter cell-specific Ace2 ...

  3. A two-stage approach in solving the state probabilities of the multi-queue M/G/1 model

    Science.gov (United States)

    Chen, Mu-Song; Yen, Hao-Wei

    2016-04-01

    The M/G/1 model is the fundamental basis of the queueing system in many network systems. Usually, the study of the M/G/1 is limited by the assumption of single queue and infinite capacity. In practice, however, these postulations may not be valid, particularly when dealing with many real-world problems. In this paper, a two-stage state-space approach is devoted to solving the state probabilities for the multi-queue finite-capacity M/G/1 model, i.e. q-M/G/1/Ki with Ki buffers in the ith queue. The state probabilities at departure instants are determined by solving a set of state transition equations. Afterward, an embedded Markov chain analysis is applied to derive the state probabilities with another set of state balance equations at arbitrary time instants. The closed forms of the state probabilities are also presented with theorems for reference. Applications of Little's theorem further present the corresponding results for queue lengths and average waiting times. Simulation experiments have demonstrated the correctness of the proposed approaches.

  4. A quadratic programming method for optimal degree reduction of Bézier curves with G1-conitnuity

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This paper presents a quadratic programming method for optimal multi-degree reduction of Bézier curves with G1-continuity. The L2 and l2 measures of distances between the two curves are used as the objective functions. The two additional parameters, available from the coincidence of the oriented tangents, are constrained to be positive so as to satisfy the solvability condition. Finally, degree reduction is changed to solve a quadratic problem of two parameters with linear constraints. Applica

  5. 26 CFR 1.402(g)-1 - Limitation on exclusion for elective deferrals.

    Science.gov (United States)

    2010-04-01

    ... 1, 2006 edition of 26 CFR Part 1). (ii) Method of allocating income. A plan may use any reasonable.... 1.402(g)-1 Section 1.402(g)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Pension, Profit-Sharing, Stock Bonus Plans, Etc. §...

  6. A hyperactive transcriptional state marks genome reactivation at the mitosis-G1 transition.

    Science.gov (United States)

    Hsiung, Chris C-S; Bartman, Caroline R; Huang, Peng; Ginart, Paul; Stonestrom, Aaron J; Keller, Cheryl A; Face, Carolyne; Jahn, Kristen S; Evans, Perry; Sankaranarayanan, Laavanya; Giardine, Belinda; Hardison, Ross C; Raj, Arjun; Blobel, Gerd A

    2016-06-15

    During mitosis, RNA polymerase II (Pol II) and many transcription factors dissociate from chromatin, and transcription ceases globally. Transcription is known to restart in bulk by telophase, but whether de novo transcription at the mitosis-G1 transition is in any way distinct from later in interphase remains unknown. We tracked Pol II occupancy genome-wide in mammalian cells progressing from mitosis through late G1. Unexpectedly, during the earliest rounds of transcription at the mitosis-G1 transition, ∼50% of active genes and distal enhancers exhibit a spike in transcription, exceeding levels observed later in G1 phase. Enhancer-promoter chromatin contacts are depleted during mitosis and restored rapidly upon G1 entry but do not spike. Of the chromatin-associated features examined, histone H3 Lys27 acetylation levels at individual loci in mitosis best predict the mitosis-G1 transcriptional spike. Single-molecule RNA imaging supports that the mitosis-G1 transcriptional spike can constitute the maximum transcriptional activity per DNA copy throughout the cell division cycle. The transcriptional spike occurs heterogeneously and propagates to cell-to-cell differences in mature mRNA expression. Our results raise the possibility that passage through the mitosis-G1 transition might predispose cells to diverge in gene expression states.

  7. Excretion of serotype G1 rotavirus strains by asymptomatic staff: a possible source of nosocomial infection.

    Science.gov (United States)

    Barnes, Graeme L; Callaghan, Sarah L; Kirkwood, Carl D; Bogdanovic-Sakran, Nada; Johnston, Linda J; Bishop, Ruth F

    2003-06-01

    This study supports the hypothesis that feces from asymptomatic adults may provide a vehicle for the transmission of rotavirus, in addition to aerosols, hands, and fomites. The observed preferential carriage of serotype G1 strains in the adult gastrointestinal tract may explain G1 predominance and persistence in epidemiologic studies worldwide.

  8. Appendix to Power Dissipation in Division

    DEFF Research Database (Denmark)

    Liu, Wei; Nannarelli, Alberto

    This document is an appendix to the paper: Wei Liu and Alberto Nannarelli, ”Power Dissipation in Division”, Proc. of 42nd Asilomar Conference on Signals, Systems, and Computers, October 2008. The purpose of the document is to provide the necessary information for the implementation of the archite......This document is an appendix to the paper: Wei Liu and Alberto Nannarelli, ”Power Dissipation in Division”, Proc. of 42nd Asilomar Conference on Signals, Systems, and Computers, October 2008. The purpose of the document is to provide the necessary information for the implementation...

  9. Characterization of IgG1 Conformation and Conformational Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Houde, Damian; Arndt, Joseph; Domeier, Wayne; Berkowitz, Steven; Engen, John R.; (NEU); (Biogen)

    2009-04-22

    Protein function is dictated by protein conformation. For the protein biopharmaceutical industry, therefore, it is important to have analytical tools that can detect changes in protein conformation rapidly, accurately, and with high sensitivity. In this paper we show that hydrogen/deuterium exchange mass spectrometry (H/DX-MS) can play an important role in fulfilling this need within the industry. H/DX-MS was used to assess both global and local conformational behavior of a recombinant monoclonal IgG1 antibody, a major class of biopharmaceuticals. Analysis of exchange into the intact, glycosylated IgG1 (and the Fab and Fc regions thereof) showed that the molecule was folded, highly stable, and highly amenable to analysis by this method using less than a nanomole of material. With improved chromatographic methods, peptide identification algorithms and data-processing steps, the analysis of deuterium levels in peptic peptides produced after labeling was accomplished in 1--2 days. On the basis of peptic peptide data, exchange was localized to specific regions of the antibody. Changes to IgG1 conformation as a result of deglycosylation were determined by comparing exchange into the glycosylated and deglycosylated forms of the antibody. Two regions of the IgG1 (residues 236-253 and 292-308) were found to have altered exchange properties upon deglycosylation. These results are consistent with previous findings concerning the role of glycosylation in the interaction of IgG1 with Fc receptors. Moreover, the data clearly illustrate how H/DX-MS can provide important characterization information on the higher order structure of antibodies and conformational changes that these molecules may experience upon modification.

  10. Role of HLA-G1 in trophoblast cell proliferation, adhesion and invasion

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Feng, E-mail: jiangfeng1161@163.com [Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi' an 710038 (China); Zhao, Hongxi [Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi' an 710038 (China); Wang, Li [Department of Gynecology and Obstetrics, The Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853 (China); Guo, Xinyu [Assisted Reproductive Center, General Hospital of Guangzhou Military Command, Guangzhou 510010 (China); Wang, Xiaohong; Yin, Guowu [Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi' an 710038 (China); Hu, Yunsheng [Department of Orthopedics, Tangdu Hospital, The Fourth Military Medical University, Xi' an 710038 (China); Li, Yi [Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Baqiao District, Xi' an 710038 (China); Yao, Yuanqing, E-mail: yuanqingyaoxa@163.com [Department of Gynecology and Obstetrics, The Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853 (China)

    2015-02-27

    Trophoblast cells are important in embryo implantation and fetomaternal tolerance. HLA-G is specifically expressed at the maternal–fetal interface and is a regulator in pregnancy. The aim of the present study was to detect the effect of HLA-G1 on trophoblast cell proliferation, adhesion, and invasion. Human trophoblast cell lines (JAR and HTR-8/SVneo cells) were infected with HLA-G1-expressing lentivirus. After infection, HLA-G1 expression of the cells was detected by western blotting. Cell proliferation was detected by the BrdU assay. The cell cycle and apoptosis of JAR and HTR-8/SVneo cells was measured by flow cytometry (FCM). The invasion of the cells under different conditions was detected by the transwell invasion chamber assay. HLA-G1 didn't show any significant influence on the proliferation, apoptosis, adhesion, and invasion of trophocytes in normal culture conditions. However, HLA-G1 inhibited JAR and HTR-8/SVneo cells invasion induced by hepatocyte growth factor (HGF) under normal oxygen conditions. In conditions of hypoxia, HLA-G1 couldn't inhibit the induction of cell invasion by HGF. HLA-G1 is not an independent factor for regulating the trophocytes. It may play an indirect role in embryo implantation and formation of the placenta. - Highlights: • HLA-G1 could not influence trophocytes under normal conditions. • HLA-G1 inhibited cell invasion induced by HGF under normal oxygen condition. • HLA-G1 could not influence cell invasion under hypoxia conditions.

  11. Msa1 and Msa2 Modulate G1-Specific Transcription to Promote G1 Arrest and the Transition to Quiescence in Budding Yeast.

    Science.gov (United States)

    Miles, Shawna; Croxford, Matthew W; Abeysinghe, Amali P; Breeden, Linda L

    2016-06-01

    Yeast that naturally exhaust their glucose source can enter a quiescent state that is characterized by reduced cell size, and high cell density, stress tolerance and longevity. The transition to quiescence involves highly asymmetric cell divisions, dramatic reprogramming of transcription and global changes in chromatin structure and chromosome topology. Cells enter quiescence from G1 and we find that there is a positive correlation between the length of G1 and the yield of quiescent cells. The Swi4 and Swi6 transcription factors, which form the SBF transcription complex and promote the G1 to S transition in cycling cells, are also critical for the transition to quiescence. Swi6 forms a second complex with Mbp1 (MBF), which is not required for quiescence. These are the functional analogues of the E2F complexes of higher eukaryotes. Loss of the RB analogue, Whi5, and the related protein Srl3/Whi7, delays G1 arrest, but it also delays recovery from quiescence. Two MBF- and SBF-Associated proteins have been identified that have little effect on SBF or MBF activity in cycling cells. We show that these two related proteins, Msa1 and Msa2, are specifically required for the transition to quiescence. Like the E2F complexes that are quiescence-specific, Msa1 and Msa2 are required to repress the transcription of many SBF target genes, including SWI4, the CLN2 cyclin and histones, specifically after glucose is exhausted from the media. They also activate transcription of many MBF target genes. msa1msa2 cells fail to G1 arrest and rapidly lose viability upon glucose exhaustion. msa1msa2 mutants that survive this transition are very large, but they attain the same thermo-tolerance and longevity of wild type quiescent cells. This indicates that Msa1 and Msa2 are required for successful transition to quiescence, but not for the maintenance of that state. PMID:27272642

  12. Msa1 and Msa2 Modulate G1-Specific Transcription to Promote G1 Arrest and the Transition to Quiescence in Budding Yeast.

    Directory of Open Access Journals (Sweden)

    Shawna Miles

    2016-06-01

    Full Text Available Yeast that naturally exhaust their glucose source can enter a quiescent state that is characterized by reduced cell size, and high cell density, stress tolerance and longevity. The transition to quiescence involves highly asymmetric cell divisions, dramatic reprogramming of transcription and global changes in chromatin structure and chromosome topology. Cells enter quiescence from G1 and we find that there is a positive correlation between the length of G1 and the yield of quiescent cells. The Swi4 and Swi6 transcription factors, which form the SBF transcription complex and promote the G1 to S transition in cycling cells, are also critical for the transition to quiescence. Swi6 forms a second complex with Mbp1 (MBF, which is not required for quiescence. These are the functional analogues of the E2F complexes of higher eukaryotes. Loss of the RB analogue, Whi5, and the related protein Srl3/Whi7, delays G1 arrest, but it also delays recovery from quiescence. Two MBF- and SBF-Associated proteins have been identified that have little effect on SBF or MBF activity in cycling cells. We show that these two related proteins, Msa1 and Msa2, are specifically required for the transition to quiescence. Like the E2F complexes that are quiescence-specific, Msa1 and Msa2 are required to repress the transcription of many SBF target genes, including SWI4, the CLN2 cyclin and histones, specifically after glucose is exhausted from the media. They also activate transcription of many MBF target genes. msa1msa2 cells fail to G1 arrest and rapidly lose viability upon glucose exhaustion. msa1msa2 mutants that survive this transition are very large, but they attain the same thermo-tolerance and longevity of wild type quiescent cells. This indicates that Msa1 and Msa2 are required for successful transition to quiescence, but not for the maintenance of that state.

  13. Appendix - A small scale research review

    DEFF Research Database (Denmark)

    Larsen, Lea Lund

     training? This will be illustrated by describing a research design in progress at the National Centre of Competence Development, DK, regarding a program where teachers are taught Cooperative Learning as a pedagogical and didactical method. This appendix concerns the first question. In search for empiric results concerning: What do......A small scale research review This appendix provides an analysis of a small scale search for empirical studies regarding the efficacy of adult teacher training. The appendix is a part of a paper delivered at the ASEM conference, June 2009, Bergisch Gladbach, Germany. The paper tries to shed light...... for systematic reviews in the international This will be identified by a search for systematic reviews in the international clearinghouses and afterwards by a small scale mapping done in the pedagogic databaseERIC. The second question sounds: which methods can be used to measure the effects of teacher...

  14. Supplementary Appendix for: Constrained Perturbation Regularization Approach for Signal Estimation Using Random Matrix Theory

    KAUST Repository

    Suliman, Mohamed

    2016-01-01

    In this supplementary appendix we provide proofs and additional simulation results that complement the paper (constrained perturbation regularization approach for signal estimation using random matrix theory).

  15. Quarter variation and correlations of colostrum albumin, immunoglobulin G1 and G2 in dairy cows.

    Science.gov (United States)

    Samarütel, Jaak; Baumrucker, Craig R; Gross, Josef J; Dechow, Chad D; Bruckmaier, Rupert M

    2016-05-01

    A high variation in immunoglobulin G1 (IgG1) concentration in first milked quarter colostrum has been reported, but BSA quarter colostrum variation is not known. The occurrence of serum albumin in milk has been attributed to increased blood-milk barrier penetration. Reports of serum albumin binding to the Fc Receptor of the neonate, the receptor thought to be responsible for IgG1 transcytosis, suggested that a correlation with the appearance of IgG1 in colostrum of dairy cows was likely. The objective of the study was to establish the quarter colostrum concentration and mass of immunoglobulins and serum albumin. First colostrum was quarter collected within 4 h of parturition from healthy udders of 31 multiparous dairy cows. Individual quarter colostrum weight was determined and a sample of each was frozen for subsequent analysis. Concentrations of immunoglobulin G1, G2, and BSA were measured by ELISA and total mass of components was calculated. In addition, colostrum was also analysed for L-lactate dehydrogenase activity. Analysis of concentration and mass of BSA, immunoglobulin G1, G2 established that the quarter variations were different by cow, quarter and quarter within cow. Partial correlations corrected for colostrum weight indicated that BSA and IgG2 concentration and mass are closely correlated while that of BSA and IgG1 concentration and mass exhibited no correlation suggesting that BSA and IgG1 may have different transport mechanisms. Interestingly, immunoglobulin G1 and G2 concentration and mass exhibited strong correlations suggesting that also some unknown mechanism of immunoglobulin G2 appearance in colostrum is occurring. Finally, no measured protein exhibited any correlation with the activity of lactate dehydrogenase in colostrum.

  16. The Recursive Solution for Geom/G/1(E,SV) Queue with Feedback and Single Server Vacation

    Institute of Scientific and Technical Information of China (English)

    Chuan-yi Luo; Ying-hui Tang

    2011-01-01

    Using recursive method, this paper studies the queue size properties at any epoch n+ in Geom/G/i(E, SV) queueing model with feedback under LASDA (late arrival system with delayed access) setup. Some new results about the recursive expressions of queue size distribution at different epoch (n+, n, n-) are obtained.Furthermore the important relations between stationary queue size distribution at different epochs are discovered.The results are different from the relations given in M/G/1 queueing system. The model discussed in this paper can be widely applied in many kinds of communications and computer network.

  17. Cdk phosphorylation of the Ste11 transcription factor constrains differentiation-specific transcription to G1

    DEFF Research Database (Denmark)

    Kjaerulff, Søren; Andersen, Nicoline Resen; Borup, Mia Trolle;

    2007-01-01

    Eukaryotic cells normally differentiate from G(1); here we investigate the mechanism preventing expression of differentiation-specific genes outside G(1). In fission yeast, induction of the transcription factor Ste11 triggers sexual differentiation. We find that Ste11 is only active in G(1) when...... S phase. When we mutated T82 to aspartic acid, mimicking constant phosphorylation, cells no longer underwent differentiation. Conversely, changing T82 to alanine rendered Ste11-controlled transcription constitutive through the cell cycle, and allowed mating from S phase with increased frequency...

  18. Synthesis and characterization of supramolecule self-assembly polyami-doamine (PAMAM G1-G1 NH2, CO2H end group Megamer

    Directory of Open Access Journals (Sweden)

    Omid Louie

    2014-10-01

    Full Text Available Supramolecule self-assembly polyamidoamine (PAMAM dendrimer refers to the chemical sys-tems made up of a discrete number of assembled molecular subunits or components. These strat-egies involve the covalent assembly of hierarchical components reactive monomers, branch cells or dendrons around atomic or molecular cores according to divergent/convergent dendritic branching principles, systematic filling of space around a core with shells (layers of branch cells. The polydispersity index (PDI for the supramolecule megamer are pretty closed to one, are in agreement with the Poisson probability distribution. Polyamidoamine (PAMAM den-drimer G1-G1 that it was PAMAM Megamer NH2, COOH end groupsynthesized and character-ized by FT-IR, 1H NMR, 13C NMRspectra and GelPermeation Chromatography (GPC.

  19. Non-singlet spin structure function in the valon model and low-x-scaling behavior of g1NS and g1p

    Science.gov (United States)

    Taghavi-Shahri, Fatemeh; Arash, Firooz

    2010-09-01

    A next-to-leading order QCD calculation of non-singlet spin structure function, g1NS is presented within the valon representation of hadrons. In the valon model, it is assumed that a nucleon is composed of three dressed valence quarks: the valons. Each valon has its own internal structure, the valence quark with its associated sea quarks and gluons. The results are in good agreement with all available data from SMC, E143, HERMES, and with the newly released data from COMPASS experiments. It appears that the small-x tail of g1NS can be described by a single Regge-type exchange. The relevant parameter of this exchange is given. Finally we show that the polarized proton structure function has a scaling behavior at small x. The relevant parameters of this behavior are given, too.

  20. Moments of the Spin Structure Functions g_1^p and g_1^d for 0.05 < Q^2 < 3.0 GeV^2

    CERN Document Server

    Prok, Y; Burkert, V D; Deur, A; Dharmawardane, K V; Dodge, G E; Griffioen, K A; Kuhn, S E; Minehart, R; Adams, G; Amaryan, M J; Anghinolfi, M; Asryan, G; Audit, G; Avakian, H; Bagdasaryan, H; Baillie, N; Ball, J P; Baltzell, N A; Barrow, S; Battaglieri, M; Beard, K; Bedlinskiy, I; Bektasoglu, M; Bellis, M; Benmouna, N; Berman, B L; Biselli, A S; Blaszczyk, L; Boiarinov, S; Bonner, B E; Bouchigny, S; Bradford, R; Branford, D; Briscoe, W J; Brooks, W K; Bültmann, S; Butuceanu, C; Calarco, J R; Careccia, S L; Carman, D S; Casey, L; Cazes, A; Chen, S; Cheng, L; Cole, P L; Collins, P; Coltharp, P; Cords, D; Corvisiero, P; Crabb, D; Credé, V; Cummings, J P; Dale, D; Dashyan, N; De Masi, R; De Vita, R; De Sanctis, E; Degtyarenko, P V; Denizli, H; Dennis, L; Dhuga, K S; Dickson, R; Djalali, C; Doughty, D; Dugger, M; Dytman, S; Dzyubak, O P; Egiyan, H; Egiyan, K S; El Fassi, L; Elouadrhiri, L; Eugenio, P; Fatemi, R; Fedotov, G; Feldman, G; Fersh, R G; Feuerbach, R J; Forest, T A; Fradi, A; Funsten, H; Garçon, M; Gavalian, G; Gevorgyan, N; Gilfoyle, G P; Giovanetti, K L; Girod, F X; Goetz, J T; Golovatch, E; Gothe, R W; Guidal, M; Guillo, M; Guler, N; Guo, L; Gyurjyan, V; Hadjidakis, C; Hafidi, K; Hakobyan, H; Hanretty, C; Hardie, J; Hassall, N; Heddle, D; Hersman, F W; Hicks, K; Hleiqawi, I; Holtrop, M; Huertas, M; Hyde-Wright, C E; Ilieva, Y; Ireland, D G; Ishkhanov, B S; Isupov, E L; Ito, M M; Jenkins, D; Jo, H S; Johnstone, J R; Joo, K; Jüngst, H G; Kalantarians, N; Keith, C D; Kellie, J D; Khandaker, M; Kim, K Y; Kim, K; Kim, W; Klein, A; Klein, F J; Klusman, M; Kossov, M; Krahn, Z; Kramer, L H; Kubarovski, V; Kühn, J; Kuleshov, S V; Kuznetsov, V; Lachniet, J; Laget, J M; Langheinrich, J; Lawrence, D; Ji Li; Lima, A C S; Livingston, K; Lu, H Y; Lukashin, K; MacCormick, M; Marchand, C; Markov, N; Mattione, P; McAleer, S; McKinnon, B; McNabb, J W C; Mecking, B A; Mestayer, M D; Meyer, C A; Mibe, T; Mikhailov, K; Mirazita, M; Miskimen, R; Mokeev, V; Morand, L; Moreno, B; Moriya, K; Morrow, S A; Moteabbed, M; Müller, J; Munevar, E; Mutchler, G S; Nadel-Turonski, P; Nasseripour, R; Niccolai, S; Niculescu, G; Niculescu, I; Niczyporuk, B B; Niroula, M R; Niyazov, R A; Nozar, M; O'Rielly, G V; Osipenko, M; Ostrovidov, A I; Park, K; Pasyuk, E; Paterson, C; Anefalos Pereira, S; Philips, S A; Pierce, J; Pivnyuk, N; Pocanic, D; Pogorelko, O; Popa, I; Pozdniakov, S; Preedom, B M; Price, J W; Procureur, S; Protopopescu, D; Qin, L M; Raue, B A; Riccardi, G; Ricco, G; Ripani, M; Ritchie, B G; Rosner, G; Rossi, P; Rowntree, D; Rubin, P D; Sabati, F; Salamanca, J; Salgado, C; Santoro, e J P; Sapunenko, V; Schumacher, R A; Seely, M L; Serov, V S; Sharabyan, Yu G; Sharov, D; Shaw, J; Shvedunov, N V; Skabelin, A V; Smith, E S; Smith, L C; Sober, D I; Sokhan, D; Stavinsky, A; Stepanyan, S S; Stepanyan, S; Stokes, B E; Stoler, P; Strakovsky, I I; Strauch, S; Suleiman, R; Taiuti, M; Tedeschi, D J; Tkabladze, A; Tkachenko, S; Todor, L; Ungaro, M; Vineyard, M F; Vlassov, A V; Watts, D P; Weinstein, L B; Weygand, D P; Williams, M; Wolin, E; Wood, M H; Yegneswaran, A; Yun, J; Zana, L; Zhang, J; Zhao, B; Zhao, Z W

    2008-01-01

    The spin structure functions g_1 for the proton and the deuteron have been measured over a wide kinematic range in x and Q2 using 1.6 and 5.7 GeV longitudinally polarized electrons incident upon polarized NH_3 and ND_3 targets at Jefferson Lab. Scattered electrons were detected in the CEBAF Large Acceptance Spectrometer, for 0.05 < Q^2 < 5 GeV^2 and W < 3 GeV. The first moments of g_1 for the proton and deuteron are presented -- both have a negative slope at low Q2, as predicted by the extended Gerasimov-Drell-Hearn sum rule. The first result for the generalized forward spin polarizability of the proton gamma_0^p is also reported, and shows evidence of scaling above Q^2 = 1.5 GeV^2. Although the first moments of g_1 are consistent with Chiral Perturbation Theory (ChPT) calculations up to approximately Q^2 = 0.06 GeV^2, a significant discrepancy is observed between the \\gamma_0^p data and ChPT for gamma_0^p,even at the lowest Q2.

  1. Moments of the Spin Structure Functions g1p and g1d for 0.05 < Q2 < 3.0 GeV2

    Energy Technology Data Exchange (ETDEWEB)

    Prok, Yelena; Bosted, Peter; Burkert, Volker; Deur, Alexandre; Dharmawardane, Kahanawita; Dodge, Gail; Griffioen, Keith; Kuhn, Sebastian; Minehart, Ralph; Adams, Gary; Amaryan, Moscov; Amaryan, Moskov; Anghinolfi, Marco; Asryan, G.; Audit, Gerard; Avagyan, Harutyun; Baghdasaryan, Hovhannes; Baillie, Nathan; Ball, J.P.; Ball, Jacques; Baltzell, Nathan; Barrow, Steve; Battaglieri, Marco; Beard, Kevin; Bedlinskiy, Ivan; Bektasoglu, Mehmet; Bellis, Matthew; Benmouna, Nawal; Berman, Barry; Biselli, Angela; Blaszczyk, Lukasz; Boyarinov, Sergey; Bonner, Billy; Bouchigny, Sylvain; Bradford, Robert; Branford, Derek; Briscoe, William; Brooks, William; Bultmann, S.; Bueltmann, Stephen; Butuceanu, Cornel; Calarco, John; Careccia, Sharon; Carman, Daniel; Casey, Liam; Cazes, Antoine; Chen, Shifeng; Cheng, Lu; Cole, Philip; Collins, Patrick; Coltharp, Philip; Cords, Dieter; Corvisiero, Pietro; Crabb, Donald; Crede, Volker; Cummings, John; Dale, Daniel; Dashyan, Natalya; De Masi, Rita; De Vita, Raffaella; De Sanctis, Enzo; Degtiarenko, Pavel; Denizli, Haluk; Dennis, Lawrence; Dhuga, Kalvir; Dickson, Richard; Djalali, Chaden; Doughty, David; Dugger, Michael; Dytman, Steven; Dzyubak, Oleksandr; Egiyan, Hovanes; Egiyan, Kim; Elfassi, Lamiaa; Elouadrhiri, Latifa; Eugenio, Paul; Fatemi, Renee; Fedotov, Gleb; Feldman, Gerald; Fersch, Robert; Feuerbach, Robert; Forest, Tony; Fradi, Ahmed; Funsten, Herbert; Garcon, Michel; Gavalian, Gagik; Gevorgyan, Nerses; Gilfoyle, Gerard; Giovanetti, Kevin; Girod, Francois-Xavier; Goetz, John; Golovach, Evgeny; Gothe, Ralf; Guidal, Michel; Guillo, Matthieu; Guler, Nevzat; Guo, Lei; Gyurjyan, Vardan; Hadjidakis, Cynthia; Hafidi, Kawtar; Hakobyan, Hayk; Hanretty, Charles; Hardie, John; Hassall, Neil; Heddle, David; Hersman, F.; Hicks, Kenneth; Hleiqawi, Ishaq; Holtrop, Maurik; Huertas, Marco; Hyde, Charles; Ilieva, Yordanka; Ireland, David; Ishkhanov, Boris; Isupov, Evgeny; Ito, Mark; Jenkins, David; Jo, Hyon-Suk; Johnstone, John; Joo, Kyungseon; Juengst, Henry; Kalantarians, Narbe; Keith, Christopher; Kellie, James; Khandaker, Mahbubul; Kim, Kui; Kim, Kyungmo; Kim, Wooyoung; Klein, Andreas; Klein, Franz; Klusman, Mike; Kossov, Mikhail; Krahn, Zebulun; Kramer, Laird; Kubarovsky, Valery; Kuhn, Joachim; Kuleshov, Sergey; Kuznetsov, Viacheslav; Lachniet, Jeff; Laget, Jean; Langheinrich, Jorn; Lawrence, Dave; Lima, Ana; Livingston, Kenneth; Lu, Haiyun; Lukashin, K.; MacCormick, Marion; Marchand, Claude; Markov, Nikolai; Mattione, Paul; McAleer, Simeon; McKinnon, Bryan; McNabb, John; Mecking, Bernhard; Mestayer, Mac; Meyer, Curtis; Mibe, Tsutomu; Mikhaylov, Konstantin; Mirazita, Marco; Miskimen, Rory; Mokeev, Viktor; Morand, Ludyvine; Moreno, Brahim; Moriya, Kei; Morrow, Steven; Moteabbed, Maryam; Mueller, James; Munevar Espitia, Edwin; Mutchler, Gordon; Nadel-Turonski, Pawel; Nasseripour, Rakhsha; Niccolai, Silvia; Niculescu, Gabriel; Niculescu, Maria-Ioana; Niczyporuk, Bogdan; Niroula, Megh; Niyazov, Rustam; Nozar, Mina; O' Rielly, Grant; Osipenko, Mikhail; Ostrovidov, Alexander; Park, Kijun; Pasyuk, Evgueni; Paterson, Craig; Anefalos Pereira, S.; Philips, Sasha; Pierce, J.; Pivnyuk, Nikolay; Pocanic, Dinko; Pogorelko, Oleg; Popa, Iulian; Pozdnyakov, Sergey; Preedom, Barry; Price, John; Procureur, Sebastien; Protopopescu, Dan; Qin, Liming; Raue, Brian; Riccardi, Gregory; Ricco, Giovanni; Ripani, Marco; Ritchie, Barry; Rosner, Guenther; Rossi, Patrizia; Rowntree, David; Rubin, Philip; Sabatie, Franck; Salamanca, Julian; Salgado, Carlos; Santoro, Joseph; Sapunenko, Vladimir; Schumacher, Reinhard; Seely, Mikell; Serov, Vladimir; Sharabian, Youri; Sharov, Dmitri; Shaw, Jeffrey; Shvedunov, Nikolay; Skabelin, Alexander; Smith, Elton; Smith, Lee; Sober, Daniel; Sokhan, Daria; Stavinskiy, Aleksey; Stepanyan, Samuel; Stepanyan, Stepan; Stokes, Burnham; Stoler, Paul; Strakovski, Igor; Strauch, Steffen; Suleiman, Riad; Taiuti, Mauro; Tedeschi, David; Tkabladze, Avtandil; Tkachenko, Svyatoslav; Todor, Luminita; Ungaro, Maurizio; V

    2009-02-01

    The spin structure functions $g_1$ for the proton and the deuteron have been measured over a wide kinematic range in $x$ and \\Q2 using 1.6 and 5.7 GeV longitudinally polarized electrons incident upon polarized NH$_3$ and ND$_3$ targets at Jefferson Lab. Scattered electrons were detected in the CEBAF Large Acceptance Spectrometer, for $0.05 < Q^2 < 5 $\\ GeV$^2$ and $W < 3$ GeV. The first moments of $g_1$ for the proton and deuteron are presented -- both have a negative slope at low \\Q2, as predicted by the extended Gerasimov-Drell-Hearn sum rule. The first result for the generalized forward spin polarizability of the proton $\\gamma_0^p$ is also reported, and shows evidence of scaling above $Q^2$ = 1.5 GeV$^2$. Although the first moments of $g_1$ are consistent with Chiral Perturbation Theory (\\ChPT) calculations up to approximately $Q^2 = 0.06$ GeV$^2$, a significant discrepancy is observed between the $\\gamma_0^p$ data and \\ChPT\\ for $\\gamma_0^p$,even at the lowest \\Q2.

  2. 新生佳能PowerShot G1 X Mark Ⅱ

    Institute of Scientific and Technical Information of China (English)

    2014-01-01

    佳能推出大底化的PowerShot G1X后,市场反响不错。最近登场的升级版PowerShot G1 X Mark Ⅱ,改变由内至外。在外形上,G1 X Mark Ⅱ取消光学取景器,身段更加秀气,造型类似PowerShotS系列。整机重量为553g,质感良好。同时G1 X Mark Ⅱ的翻转屏幕有所改进,为更常见的上下翻转式。

  3. Redox-mediated bypass of restriction point via skipping of G1pm

    Directory of Open Access Journals (Sweden)

    Greene James J

    2006-07-01

    Full Text Available Abstract Background It is well known that cancer cells bypass the restriction point, R, and undergo uncontrolled cell proliferation. Hypothesis and evidence We suggest here that fibrosarcoma cells enter G1ps directly from M, skipping G1pm, hence bypassing R, in response to redox modulation. Evidence is presented from the published literature that demonstrate a shortening of the cycle period of transformed fibroblasts (SV-3T3 compared to the nontransformed 3T3 fibroblasts, corresponding to the duration of G1pm in the 3T3 fibroblasts. Evidence is also presented that demonstrate that redox modulation can induce the CUA-4 fibroblasts to bypass R, resulting in a cycle period closely corresponding to the cycle period of fibrosarcoma cells (HT1080. Conclusion The evidence supports our hypothesis that a low internal redox potential can cause fibrosarcoma cells to skip the G1pm phase of the cell cycle.

  4. Evaluation of Overseas Mining Investment Risk Based on G1-Grey Correlation Method%基于G1-灰色关联法的矿业企业对外投资风险评价研究

    Institute of Scientific and Technical Information of China (English)

    吴旺; 杨仕教

    2016-01-01

    针对全球矿业经济转型期我国矿业企业“走出去”投资所面临的新机遇,在对矿业企业对外投资风险影响因素进行全面辨识、分析和筛选的基础上,建立了同时考虑外部和内部因素的矿业企业对外投资风险评价三级指标体系。本文采用G1法对评价指标进行赋权,并结合灰色关联分析法,构建了基于G1-灰色关联分析法的矿业企业对外投资风险评价模型。最后通过算例分析,验证了所构建的投资风险评价模型的有效性和适用性。%With the new opportunities in global mining economic transformation faced by the mining enterprises of our country“going out”,a set of overseas mining investment risk evaluation index system considering external and internal factors of mining enterprises is established on the basis of identification,analysis and selection of the risk factors for over-seas mining investment.This paper adopts the G1 method to determine the weight of each evaluation index,and an overseas investment risk evaluation model of mining enterprises based on G1 method and grey relational analysis method is constructed.Finally,an instance analysis is studied to prove the validity and applicability of this risk evaluation model.

  5. The amrG1 gene is involved in the activation of acetate in Corynebacterium glutamicum

    Institute of Scientific and Technical Information of China (English)

    RUAN Hong; R. Gerstmeir; S. Schnicke; B.J. Eikmanns

    2005-01-01

    During growth of Corynebacterium glutamicum on acetate as its carbon and energy source, the expression of the pta-ack operon is induced, coding for the acetate-activating enzymes, which are phosphotransacetylase (PTA) and acetate kinase (AK). By transposon rescue, we identified the two genes amrG1 and amrG2 found in the deregulated transposon mutant C. glutamicum G25. The amrG1 gene (NCBI-accession: AF532964) has a size of 732 bp, encoding a polypeptide of 243 amino acids and apparently is partially responsible for the regulation of acetate metabolism in C. glutamicum. We constructed an in-frame deletion mutant and an overexpressing strain of amrG1 in the C. glutamicum ATCC13032 wildtype. The strains were then analyzed with respect to their enzyme activities of PTA and AK during growth on glucose, acetate and glucose or acetate alone as carbon sources. Compared to the parental strain, the amrG1 deletion mutant showed higher specific AK and PTA activities during growth on glucose but showed the same high specific activities of AK and PTA on medium containing acetate plus glucose and on medium containing acetate. In contrast to the gene deletion, overexpression of the amrG1 gene in C. glutamicum 13032 had the adverse regulatory effect. These results indicate that the amrG1 gene encodes a repressor or co-repressor of the pta-ack operon.

  6. IgG1 Fc N-glycan galactosylation as a biomarker for immune activation.

    Science.gov (United States)

    de Jong, Sanne E; Selman, Maurice H J; Adegnika, Ayola A; Amoah, Abena S; van Riet, Elly; Kruize, Yvonne C M; Raynes, John G; Rodriguez, Alejandro; Boakye, Daniel; von Mutius, Erika; Knulst, André C; Genuneit, Jon; Cooper, Philip J; Hokke, Cornelis H; Wuhrer, Manfred; Yazdanbakhsh, Maria

    2016-01-01

    Immunoglobulin G (IgG) Fc N-glycosylation affects antibody-mediated effector functions and varies with inflammation rooted in both communicable and non-communicable diseases. Worldwide, communicable and non-communicable diseases tend to segregate geographically. Therefore, we studied whether IgG Fc N-glycosylation varies in populations with different environmental exposures in different parts of the world. IgG Fc N-glycosylation was analysed in serum/plasma of 700 school-age children from different communities of Gabon, Ghana, Ecuador, the Netherlands and Germany. IgG1 galactosylation levels were generally higher in more affluent countries and in more urban communities. High IgG1 galactosylation levels correlated with low total IgE levels, low C-reactive protein levels and low prevalence of parasitic infections. Linear mixed modelling showed that only positivity for parasitic infections was a significant predictor of reduced IgG1 galactosylation levels. That IgG1 galactosylation is a predictor of immune activation is supported by the observation that asthmatic children seemed to have reduced IgG1 galactosylation levels as well. This indicates that IgG1 galactosylation levels could be used as a biomarker for immune activation of populations, providing a valuable tool for studies examining the epidemiological transition from communicable to non-communicable diseases. PMID:27306703

  7. IgG1 Fc N-glycan galactosylation as a biomarker for immune activation

    Science.gov (United States)

    de Jong, Sanne E.; Selman, Maurice H. J.; Adegnika, Ayola A.; Amoah, Abena S.; van Riet, Elly; Kruize, Yvonne C. M.; Raynes, John G.; Rodriguez, Alejandro; Boakye, Daniel; von Mutius, Erika; Knulst, André C.; Genuneit, Jon; Cooper, Philip J.; Hokke, Cornelis H.; Wuhrer, Manfred; Yazdanbakhsh, Maria

    2016-01-01

    Immunoglobulin G (IgG) Fc N-glycosylation affects antibody-mediated effector functions and varies with inflammation rooted in both communicable and non-communicable diseases. Worldwide, communicable and non-communicable diseases tend to segregate geographically. Therefore, we studied whether IgG Fc N-glycosylation varies in populations with different environmental exposures in different parts of the world. IgG Fc N-glycosylation was analysed in serum/plasma of 700 school-age children from different communities of Gabon, Ghana, Ecuador, the Netherlands and Germany. IgG1 galactosylation levels were generally higher in more affluent countries and in more urban communities. High IgG1 galactosylation levels correlated with low total IgE levels, low C-reactive protein levels and low prevalence of parasitic infections. Linear mixed modelling showed that only positivity for parasitic infections was a significant predictor of reduced IgG1 galactosylation levels. That IgG1 galactosylation is a predictor of immune activation is supported by the observation that asthmatic children seemed to have reduced IgG1 galactosylation levels as well. This indicates that IgG1 galactosylation levels could be used as a biomarker for immune activation of populations, providing a valuable tool for studies examining the epidemiological transition from communicable to non-communicable diseases. PMID:27306703

  8. 研究M/G/1排队的一个新的向量马氏过程%A New Vector Markov Process for M/G/1 Queue

    Institute of Scientific and Technical Information of China (English)

    严庆强; 史定华; 郭兴国

    2005-01-01

    In this paper, by considering the stochastic process of the busy period and the idle period, and introducing the unfinished work as a supplementary variable, a new vector Markov process was presented to study the M/G/1 queue again. Through establishing and solving the density evolution equations, the busy-period distribution, and the stationary distributions of waiting time and queue length were obtained. In addition, the stability condition of this queue system was given by means of an imbedded renewal process.

  9. 26 CFR 6a.6652(g)-1 - Failure to make return or furnish statement required under section 6039C.

    Science.gov (United States)

    2010-04-01

    ... required under section 6039C. 6a.6652(g)-1 Section 6a.6652(g)-1 Internal Revenue INTERNAL REVENUE SERVICE... OMNIBUS RECONCILIATION ACT OF 1980 § 6a.6652(g)-1 Failure to make return or furnish statement required... limitation under § 6a.6652(g)-1(b)(3) with respect to failure to meet the requirements of section 6039C(c),...

  10. Binomial Schedule for an M/G/1 Type Queueing System with an Unreliable Server under N-Policy

    Directory of Open Access Journals (Sweden)

    Lotfi Tadj

    2014-01-01

    Full Text Available We consider in this paper an M/G/1 type queueing system with the following extensions. First, the server is unreliable and is subject to random breakdowns. Second, the server also implements the well-known N-policy. Third, instead of a Bernoulli vacation schedule, the more general notion of binomial schedule with K vacations is applied. A cost function with two decision variables is developed. A numerical example shows the effect of the system parameters on the optimal management policy.

  11. Transient Behaviour of Batch Arrival Queue with N-Policy and Single Vacation (Mx/G/1/N-POLICY)

    Science.gov (United States)

    Solanki, Anjana

    2009-07-01

    In this paper Mx/G/1 queuing system with N-policy and single vacation is considered. As soon as the system becomes empty, the server leaves the system for a vacation of random length V. When he returns from the vacation, if the system size is greater then or equal to predetermined value N (threshold), he begins to serve the customers. If not, the server waits in the system until the system size reaches or exceeds N. Here the time dependent system size distribution is obtained.

  12. Paper electronics.

    Science.gov (United States)

    Tobjörk, Daniel; Österbacka, Ronald

    2011-05-01

    Paper is ubiquitous in everyday life and a truly low-cost substrate. The use of paper substrates could be extended even further, if electronic applications would be applied next to or below the printed graphics. However, applying electronics on paper is challenging. The paper surface is not only very rough compared to plastics, but is also porous. While this is detrimental for most electronic devices manufactured directly onto paper substrates, there are also approaches that are compatible with the rough and absorptive paper surface. In this review, recent advances and possibilities of these approaches are evaluated and the limitations of paper electronics are discussed.

  13. Natural uranium-graphite system. Critial experiments on the G1 reactor

    International Nuclear Information System (INIS)

    A number of experiments have been performed during the start up period of the G1 (1956) and G2 (1958) reactors in Marcoule, both on their lattices and on different lattices (hollow rods, clusters, under moderated lattices). The first chapter gives a thorough description of the two reactors. The second chapter deals with buckling measurements, both absolute (flux plots) and relative by the method of progressive substitution. The experimental results are summarised in Table VI. The third chapter contains a number of other measurements performed on G1. (author)

  14. On the first $G_1$ stiff fluid spike solution in General Relativity

    CERN Document Server

    Coley, Alan; Lim, Woei Chet

    2016-01-01

    Using the Geroch transformation we obtain the first example of a stiff fluid solution to the Einstein field equations in a closed form exhibiting a spacelike $G_1$ group of symmetries (i.e., with a single isometry). This new solution can be interpreted as an exact example of a close-to-Friedmann-Lemaitre (perturbative) solution. The exact solution is the first (non-null) $G_1$ solution found, and exhibits a spike crossing which persists to the past, which allows us to better understand spike crossings in the context of structure formation.

  15. TOR controls translation initiation and early G1 progression in yeast.

    OpenAIRE

    Barbet, N.C.; Schneider, U.; Helliwell, S B; Stansfield, I; Tuite, M F; Hall, M N

    1996-01-01

    Saccharomyces cerevisiae cells treated with the immunosuppressant rapamycin or depleted for the targets of rapamycin TOR1 and TOR2 arrest growth in the early G1 phase of the cell cycle. Loss of TOR function also causes an early inhibition of translation initiation and induces several other physiological changes characteristic of starved cells entering stationary phase (G0). A G1 cyclin mRNA whose translational control is altered by substitution of the UBI4 5' leader region (UBI4 is normally t...

  16. Kernel Density Estimation for Interdeparture Time of GI/G/1 Queues

    Directory of Open Access Journals (Sweden)

    Hsing Luh

    2005-01-01

    Full Text Available The departure process of a single queue has been studied since the 1960s. Due to its inherent complexity, closed form solutions for the distribution of the departure process are nearly intractable. In this study, kernel type estimators of the density of interdeparture time in a GI/G/1 queue are studied. Uniform strong consistency of the estimators in a GI/G/1 queue and their rates of convergence are obtained. The stochastic processes are shown to satisfy the strong mixing condition with random instants of sampling. With the analysis presented, we provide a novel analytic tool for studying the departure process in a general queueing model.

  17. Bayesian Inference and Prediction in an M/G/1 with Optional Second Service

    NARCIS (Netherlands)

    Mohammadi, A.; Salehi-Rad, M. R.

    2012-01-01

    In this article, we exploit the Bayesian inference and prediction for an M/G/1 queuing model with optional second re-service. In this model, a service unit attends customers arriving following a Poisson process and demanding service according to a general distribution and some of customers need to r

  18. 26 CFR 1.414(g)-1 - Definition of plan administrator.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 5 2010-04-01 2010-04-01 false Definition of plan administrator. 1.414(g)-1... Definition of plan administrator. (a) In general. For purposes of part I of subchapter D of chapter 1 of the... specifically designates a person or a group of persons as plan administrator, the person or group of...

  19. Learning Progression of Ecological System Reasoning for Lower Elementary (G1-4) Students

    Science.gov (United States)

    Hokayem, Hayat Al

    2012-01-01

    In this study, I utilized a learning progression framework to investigate lower elementary students (G1-4) systemic reasoning in ecology and I related students reasoning to their sources of knowledge. I used semi-structured interviews with 44 students from first through fourth grade, four teachers, and eight parents. The results revealed that a…

  20. Differential regulation of the cellular response to DNA double-strand breaks in G1

    DEFF Research Database (Denmark)

    Barlow, Jacqueline H; Lisby, Michael; Rothstein, Rodney

    2008-01-01

    Double-strand breaks (DSBs) are potentially lethal DNA lesions that can be repaired by either homologous recombination (HR) or nonhomologous end-joining (NHEJ). We show that DSBs induced by ionizing radiation (IR) are efficiently processed for HR and bound by Rfa1 during G1, while endonuclease...

  1. 17 CFR 240.12g-1 - Exemption from section 12(g).

    Science.gov (United States)

    2010-04-01

    ... pursuant to section 12(g)(1) if on the last day of its most recent fiscal year the issuer had total assets... quoted in an automated inter-dealer quotation system. (Secs. 6, 7, 8, 10, 19(a), 48 Stat. 78, 79, 81,...

  2. Data of evolutionary structure change: 1N32G-1IQVA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available ain>G 1N32G INKIM---RDGKK e>HHHH --- H...Chain> 1IQVA INKVMRSGGSSKK ...>HHHHH H> ATOM 293 CA ILE A 71 40.019 9.985 12....in> 1N32G QRPER---RAAVR > --- HHHH... 1IQVA AKCYRTKMSFAEA >HHHHH HHHH

  3. 26 CFR 1.430(g)-1 - Valuation date and valuation of plan assets.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 5 2010-04-01 2010-04-01 false Valuation date and valuation of plan assets. 1... (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Certain Stock Options § 1.430(g)-1 Valuation date and valuation of plan assets. (a) In general—(1) Overview. This section provides rules relating to a...

  4. Old stellar population synthesis: new age and mass estimates for Mayall Ⅱ = G1

    Institute of Scientific and Technical Information of China (English)

    Jun Ma; Richard de Grijs; Zhou Fan; Soo-Chang Rey; Zhen-Yu Wu; Xu Zhou; Jiang-Hua Wu; Zhao-Ji Jiang; Jian-Sheng Chen; Kyungsook Lee; Sangmo Tony Sohn

    2009-01-01

    Mayall Ⅱ = G1 is one of the most luminous globular clusters (GCs) in M31. Here, we determine its age and mass by comparing multicolor photometry with theo-retical stellar population synthesis models. Based on far- and near-ultraviolet GALEX photometry, broad-band UBV RI, and infrared JHK8 2MASS data, we construct the most extensive spectral energy distribution of G1 to date, spanning the wavelength range from 1538 to 20000A. A quantitative comparison with a variety of simple stellar pop-ulation (SSP) models yields a mean age which is consistent with G1 being among the oldest building blocks of M31 and having formed within ~1.7Gyr after the Big Bang. Irrespective of the SSP model or stellar initial mass function adopted, the resulting mass estimates (of order 107M⊙) indicate that G1 is one of the most massive GCs in the Local Group. However, we speculate that the cluster's exceptionally high mass suggests that it may not be a genuine GC. Our results also suggest that G1 may contain, on average, (1.65±0.63) × 102L⊙ far-ultraviolet-bright, hot, extreme horizontal-branch stars, depend-ing on the adopted SSP model. In addition, we demonstrate that extensive multi-passband photometry coupled with SSP analysis enables one to obtain age estimates for old SSPs that have similar accuracies as those from integrated spectroscopy or resolved stellar pho-tometry, provided that some of the free parameters can be constrained independently.

  5. Identification of G1-regulated genes in normally cycling human cells.

    Directory of Open Access Journals (Sweden)

    Maroun J Beyrouthy

    Full Text Available BACKGROUND: Obtaining synchronous cell populations is essential for cell-cycle studies. Methods such as serum withdrawal or use of drugs which block cells at specific points in the cell cycle alter cellular events upon re-entry into the cell cycle. Regulatory events occurring in early G1 phase of a new cell cycle could have been overlooked. METHODOLOGY AND FINDINGS: We used a robotic mitotic shake-off apparatus to select cells in late mitosis for genome-wide gene expression studies. Two separate microarray experiments were conducted, one which involved isolation of RNA hourly for several hours from synchronous cell populations, and one experiment which examined gene activity every 15 minutes from late telophase of mitosis into G1 phase. To verify synchrony of the cell populations under study, we utilized methods including BrdU uptake, FACS, and microarray analyses of histone gene activity. We also examined stress response gene activity. Our analysis enabled identification of 200 early G1-regulated genes, many of which currently have unknown functions. We also confirmed the expression of a set of genes candidates (fos, atf3 and tceb by qPCR to further validate the newly identified genes. CONCLUSION AND SIGNIFICANCE: Genome-scale expression analyses of the first two hours of G1 in naturally cycling cells enabled the discovery of a unique set of G1-regulated genes, many of which currently have unknown functions, in cells progressing normally through the cell division cycle. This group of genes may contain future targets for drug development and treatment of human disease.

  6. An M[x]/G/1 Retrial G-queue with Single Vacation Subject to the Server Breakdown and Repair

    Institute of Scientific and Technical Information of China (English)

    Shu-ping YANG; Jin-biao WU; Zai-ming LIU

    2013-01-01

    An M[X]/G/1 retrial G-queue with single vacation and unreliable server is investigated in this paper.Arrivals of positive customers form a compound Poisson process,and positive customers receive service immediately if the server is free upon their arrivals; Otherwise,they may enter a retrial orbit and try their luck after a random time interval.The arrivals of negative customers form a Poisson process.Negative customers not only remove the customer being in service,but also make the server under repair.The server leaves for a single vacation as soon as the system empties.In this paper,we analyze the ergodical condition of this model.By applying the supplementary variables method,we obtain the steady-state solutions for both queueing measures and reliability quantities.

  7. Study of the extraction of residual heat for a steam generator in the presence of incondensibles modeling with TRACE: PKL experiment III G1.1

    International Nuclear Information System (INIS)

    This paper made the simulation of the PKL III G1.1 experiment using SNAP interface and the TRACE code. This experiment aims to essentially the study of the extraction of the residual heat of the steam generator in the presence of gases incondensibles.

  8. Situation analysis of physical independence of the equipment and safety circuits of Almaraz NPP regarding R.G. 1.75 rev.3 (2005)

    International Nuclear Information System (INIS)

    Situation analysis of physical independence of the electrical equipment and circuits CN safety Almaraz about R.G. 1.75 rev. 3. (2005) The aim of this paper is to present the work done in the analysis of the physical separation of redundant safety electrical equipment (emergency diesel generators, medium voltage, electrical cabinets, etc.) and physical separation of circuits and electrical conduits.

  9. 26 CFR 1.904(g)-1T - Overall domestic loss and the overall domestic loss account (temporary).

    Science.gov (United States)

    2010-04-01

    ... loss account (temporary). 1.904(g)-1T Section 1.904(g)-1T Internal Revenue INTERNAL REVENUE SERVICE... United States § 1.904(g)-1T Overall domestic loss and the overall domestic loss account (temporary). (a... accounts for purposes of section 904(g). Section 1.904(g)-2T provides rules for recapturing the balance...

  10. 26 CFR 1.1402(g)-1 - Treatment of certain remuneration erroneously reported as net earnings from self-employment.

    Science.gov (United States)

    2010-04-01

    ... reported as net earnings from self-employment. 1.1402(g)-1 Section 1.1402(g)-1 Internal Revenue INTERNAL...-Employment Income § 1.1402(g)-1 Treatment of certain remuneration erroneously reported as net earnings from... request, and should indicate clearly that it is a request that, pursuant to section 1402(g) of the...

  11. PAPER LANDSCAPES

    Institute of Scientific and Technical Information of China (English)

    Lorna Mansley

    2008-01-01

    <正>Take a sheet of paper, a pair of scissors or a knife, a vivid imagination and see what you can create. Synonymous with Chinese folkart, paper cutting no longer seems to be a flourishing craft amongst China’s younger generations of creatives, though its motifs can

  12. Regulation of the G1 phase of the mammalian cell cycle

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    In any multi-cellular organism, the balance between cell division and cell death maintains a constant cell num ber. Both cell division cycle and cell death are highly regulated events. Whether the cell will proceed through the cycle or not, depends upon whether the conditions re quired at the checkpoints during the cycle are filfilled. In higher eucaryotic cells, such as mammalian cells, signals that arrest the cycle usually act at a G1 checkpoint. Cells that pass this restriction point are committed to complete the cycle. Regulation of the G1 phase of the cell cycle is extremely complex and involves many different families of proteins such as retinoblastoma family, cyclin dependent kinases, cyclins, and cyclin kinase inhibitors.

  13. OPTIMAL CONTROL OF AN M/G/1 RETRIAL QUEUE WITH VACATIONS

    Institute of Scientific and Technical Information of China (English)

    Arnar AISSANI

    2008-01-01

    In this note, we consider an M/G/1 retrial queue with server vacations, when retrial times, servicetimes and vacation times are arbitrary distributed. The distribution of the number of customers in the system in stationary regime is obtained in terms of generating function. Next, we give heavy traffic approximation of such distribution. We show that the system size can be decomposed into two random variables, one of which corresponds to the system size of the ordinary M/G/1 FIFO queue without vacation. Such a stochastic decomposition property is useful for the computation of performance measures of interest. Finally, we solve simple problems of optimal control of vacation and retrial policies.

  14. The MAP, M/G1,G2/1 queue with preemptive priority

    Directory of Open Access Journals (Sweden)

    Bong Dae Choi

    1997-01-01

    Full Text Available We consider the MAP, M/G1,G2/1 queue with preemptive resume priority, where low priority customers arrive to the system according to a Markovian arrival process (MAP and high priority customers according to a Poisson process. The service time density function of low (respectively: high priority customers is g1(x (respectively: g2(x. We use the supplementary variable method with Extended Laplace Transforms to obtain the joint transform of the number of customers in each priority queue, as well as the remaining service time for the customer in service in the steady state. We also derive the probability generating function for the number of customers of low (respectively, high priority in the system just after the service completion epochs for customers of low (respectively, high priority.

  15. Expansion of the Youngest Galactic Supernova Remnant G1.9+0.3

    CERN Document Server

    Carlton, A K; Reynolds, S P; Hwang, U; Petre, R; Green, D A; Krishnamurthy, K; Willett, R

    2011-01-01

    We present a measurement of the expansion and brightening of G1.9+0.3, the youngest Galactic supernova remnant, comparing Chandra X-ray images obtained in 2007 and 2009. A simple uniform expansion model describes the data well, giving an expansion rate of 0.642 +/- 0.049 % yr^-1, and a flux increase of 1.7 +/- 1.0 % yr^-1. Without deceleration, the remnant age would then be 156 +/- 11 yr, consistent with earlier results. Since deceleration must have occurred, this age is an upper limit; we estimate an age of about 110 yr, or an explosion date of about 1900. The flux increase is comparable to reported increases at radio wavelengths. G1.9+0.3 is the only Galactic supernova remnant increasing in flux, with implications for the physics of electron acceleration in shock waves

  16. Developing an Intelligent Automatic Appendix Extraction Method from Ultrasonography Based on Fuzzy ART and Image Processing

    Directory of Open Access Journals (Sweden)

    Kwang Baek Kim

    2015-01-01

    Full Text Available Ultrasound examination (US does a key role in the diagnosis and management of the patients with clinically suspected appendicitis which is the most common abdominal surgical emergency. Among the various sonographic findings of appendicitis, outer diameter of the appendix is most important. Therefore, clear delineation of the appendix on US images is essential. In this paper, we propose a new intelligent method to extract appendix automatically from abdominal sonographic images as a basic building block of developing such an intelligent tool for medical practitioners. Knowing that the appendix is located at the lower organ area below the bottom fascia line, we conduct a series of image processing techniques to find the fascia line correctly. And then we apply fuzzy ART learning algorithm to the organ area in order to extract appendix accurately. The experiment verifies that the proposed method is highly accurate (successful in 38 out of 40 cases in extracting appendix.

  17. Aircraft measurements of aerosol properties during GoAmazon - G1 and HALO inter-comparison

    Science.gov (United States)

    Mei, F.; Cecchini, M. A.; Wang, J.; Tomlinson, J. M.; Comstock, J. M.; Hubbe, J. M.; Pekour, M. S.; Machado, L.; Wendisch, M.; Longo, K.; Martin, S. T.; Schmid, B.; Weinzierl, B.; Krüger, M. L.; Zöger, M.

    2015-12-01

    Currently, the indirect effects of atmospheric aerosols remain the most uncertain components in forcing of climate change over the industrial period (IPCC, 2013). This large uncertainty is partially a result of our incomplete understanding of the ability of particles to form cloud droplets under atmospherically relevant supersaturations. One objective of the US Department of Energy (DOE) Green Ocean Amazon Project (GoAmazon2014/5) is to understand the influence of the emission from Manaus, a tropical megacity, on aerosol size, concentration, and chemical composition, and their impact on cloud condensation nuclei (CCN) spectrum. The GoAmazon2014/5 study was an international campaign with the collaboration efforts from US, Brazil and Germany. During the intensive operation period, in the dry season (Sep. 1st - Oct. 10th, 2014), aerosol concentration, size distributions, and CCN spectra, both under pristine conditions and inside the Manaus plume, were characterized in-situ from the DOE Gulfstream-1 (G-1) research aircraft and German HALO aircraft during 4 coordinated flights on Sep. 9th, Sep. 16th, Sep 21st and Oct. 1st, 2014. During those four flights, aerosol number concentrations and CCN concentrations at two supersaturations (0.25% and 0.5%) were measured by condensation particle counters (CPCs) and a DMT dual column CCN counter onboard both G-1 and HALO. Aerosol size distribution was also measured by a Fast Integrated Mobility Spectrometer (FIMS) aboard the G-1 and is compared with the size distribution from Ultra High Sensitivity Aerosol Spectrometer - Airborne (UHSAS-A, DMT), which were deployed both on the G-1 and the HALO. Good agreement between the aerosol properties measured from the two aircraft has been achieved. The vertical profiles of aerosol size distribution and CCN spectrum will be discussed.

  18. Cell size at S phase initiation: an emergent property of the G1/S network.

    Directory of Open Access Journals (Sweden)

    Matteo Barberis

    2007-04-01

    Full Text Available The eukaryotic cell cycle is the repeated sequence of events that enable the division of a cell into two daughter cells. It is divided into four phases: G1, S, G2, and M. Passage through the cell cycle is strictly regulated by a molecular interaction network, which involves the periodic synthesis and destruction of cyclins that bind and activate cyclin-dependent kinases that are present in nonlimiting amounts. Cyclin-dependent kinase inhibitors contribute to cell cycle control. Budding yeast is an established model organism for cell cycle studies, and several mathematical models have been proposed for its cell cycle. An area of major relevance in cell cycle control is the G1 to S transition. In any given growth condition, it is characterized by the requirement of a specific, critical cell size, PS, to enter S phase. The molecular basis of this control is still under discussion. The authors report a mathematical model of the G1 to S network that newly takes into account nucleo/cytoplasmic localization, the role of the cyclin-dependent kinase Sic1 in facilitating nuclear import of its cognate Cdk1-Clb5, Whi5 control, and carbon source regulation of Sic1 and Sic1-containing complexes. The model was implemented by a set of ordinary differential equations that describe the temporal change of the concentration of the involved proteins and protein complexes. The model was tested by simulation in several genetic and nutritional setups and was found to be neatly consistent with experimental data. To estimate PS, the authors developed a hybrid model including a probabilistic component for firing of DNA replication origins. Sensitivity analysis of PS provides a novel relevant conclusion: PS is an emergent property of the G1 to S network that strongly depends on growth rate.

  19. Analysis of aflatoxins B1 and G1 in maize by quechers

    OpenAIRE

    Bursić Vojislava P.; Vuković Gorica Lj.; Jajić Igor M.; Lazić Sanja D.; Kara Magdalena H.; Čolović Radmilo R.; Vukmirović Đuro M.

    2013-01-01

    A reliable and easy method has been developed for the determination of aflatoxins B1 and G2 in maize samples. High performance liquid chromatography coupled with FLD (HPLC-FLD) with photochemical derivatization was used. Mycotoxins were extracted from maize using a QuEChERS-based extraction procedure. The optimized analytical conditions were evaluated in terms of recoveries, reproducibility, LOD, LOQ and linearity for aflatoxin B1 and aflatoxin G1 in maize. Extraction, chromatographic a...

  20. FUNCTIONAL ANALYSIS METHOD FOR THE M/G/1 QUEUEING MODEL WITH OPTIONAL SECOND SERVICE

    Institute of Scientific and Technical Information of China (English)

    艾尼·吾甫尔; 艾合买提·卡斯木

    2014-01-01

    By studying the spectral properties of the underlying operator corresponding to the M/G/1 queueing model with optional second service we obtain that the time-dependent solution of the model strongly converges to its steady-state solution. We also show that the time-dependent queueing size at the departure point converges to the corresponding steady-state queueing size at the departure point.

  1. Nonperturbative renormalization of the Delta-S=1 weak Hamiltonian including the G_1 operator

    CERN Document Server

    McGlynn, Greg

    2016-01-01

    Under renormalization, physical operators can mix with operators which vanish by the equations of motion. Such operators cannot contribute to matrix elements between physical states, but they contribute to operator mixing in renormalization schemes which are defined at an off-shell momentum point, such as the popular regularization-invariant schemes. For the first time, we renormalize the lattice $\\Delta S=1$ effective weak Hamiltonian taking into account the most important such operator, $G_1 \\propto \\overline s \\gamma_\

  2. Quantitative determination of G-1 in an emulsifiable concentrate by gas chromatography capillary

    International Nuclear Information System (INIS)

    G-1 is a bioactive obtained from furfural (a sugarcane derivative) which has demonstrated fungicide and bactericide effects on a broad spectrum. In this project a new capillary gas chromatography technique was developed and validated for the quantitative determination of G-1 in an emulsifiable concentrate (used in treatment of an affected skin) with view of future quality control. A capillary column for the separation, nitrogen as the carrier gas (mobile phase) and a flame ionization detector were used. Chromatographic conditions were optimized until high efficiency separation was achieved. Validation was done following the norms described by ICH. G-1 retention time under the created conditions was 4.7 minutes. This technique accomplished the principal validation parameters: linear (in the interval between 0.1 and 0.5 mg/mL), precision [repeatability (variation coefficient= 1.11%)] and intermediate precision (variation coefficient=1.17%), accuracy (covering 100%), sensitivity (the limit of detection is 0.0087mg/mL and the quantification limit is 0.0092mg/mL) and specificity (the width of the half height and symmetry factors did not show significant variations). The technique is hence rendered capable to produce satisfactory results, justifiable by the actual validation parameters.

  3. Optimized culture condition for enhancing lytic performance of waste activated sludge by Geobacillus sp. G1.

    Science.gov (United States)

    Yang, Chunxue; Zhou, Aijuan; Hou, Yanan; Zhang, Xu; Guo, Zechong; Wang, Aijie; Liu, Wenzong

    2014-01-01

    Hydrolysis is known as the rate-limiting step during waste activated sludge (WAS) digestion. The optimization of the culture conditions of Geobacillus sp. G1 for enhancing WAS hydrolysis was conducted in this study with uniform design and response surface methodology. Taking the lysis rate of Escherichia coli as the response, the Plackett-Burman design was used to screen the most important variables. Experimental results showed that the maximum predicted lysis rate of E. coli was 50.9% for 4 h treatment time with concentrations of skim milk, NaCl and NH4SO4 at 10.78, 4.36 and 11.28 g/L, respectively. The optimized dosage ratio of Geobacillus sp. G1 to WAS was 35%:65% (VG1:VWAS). Under this condition, soluble protein was increased to 695 mg chemical oxygen demand (COD)/L, which was 5.0 times higher than that obtained in the control (140 mg COD/L). The corresponding protease activity reached 1.1 Eu/mL. Scanning electron microscopy showed that abundant cells were apparently lysed with treatment of Geobacillus sp. G1.

  4. Analysis of aflatoxins B1 and G1 in maize by quechers

    Directory of Open Access Journals (Sweden)

    Bursić Vojislava P.

    2013-01-01

    Full Text Available A reliable and easy method has been developed for the determination of aflatoxins B1 and G2 in maize samples. High performance liquid chromatography coupled with FLD (HPLC-FLD with photochemical derivatization was used. Mycotoxins were extracted from maize using a QuEChERS-based extraction procedure. The optimized analytical conditions were evaluated in terms of recoveries, reproducibility, LOD, LOQ and linearity for aflatoxin B1 and aflatoxin G1 in maize. Extraction, chromatographic and detection conditions were optimized in order to increase sample sensitivity. The linearity was analyzed in the range of 0.4-20 μg/kg and the correlation coefficients (R2 were higher than 0.99 for aflatoxins B1 and G1. Blank samples were spiked at 1.0, 2.0 and 4.0 μg/kg, and the average recovery for aflatoxin G1 was 96.96±1.72% and for aflatoxin B1 it was 86.80±1.24%. RSDs were lower than 25% for both mycotoxins. LOD for both aflatoxins was 0.5 μg/kg and LOQ was 1.0 μg/kg, respectively.

  5. Molecular cloning of a gene that is necessary for G1 progression in mammalian cells

    International Nuclear Information System (INIS)

    The authors have cloned a human cDNA that complements the mutation of ts11, a temperature-sensitive (ts) mutant of the BHK hamster cell line that at the nonpermissive temperature is blocked in progression through the G1 phase of the cell growth cycle. After transfecting human chromosomal DNA into ts11 cells and selecting for cells that had acquired a non-ts phenotype, the authors screened a geonomic library constructed in the EMBL3 λ vector from a secondary non-ts transformant and isolated a recombinant phage containing human DNA sequences that were uniformly present in primary and secondary non-ts transformants. Genomic probes that recognized an mRNA of about 2 kilobases in human cells were used to isolate from a cDNA expression library two cDNA plasmids that could efficiently transform ts11 cells to a non-ts phenotype. Sequencing of one of these cDNAs revealed a single open reading frame, which could encode a 540 amino acid protein. The ts11 gene has at least two other homologs in human DNA and thus it appears to be part of a small gene/pseudogene family. Experiments with serum-synchronized cells indicate that the expression of the ts11 gene, which is necessary for G1 progression, is itself cell-cycle regulated, being induced in approximately mid-G1

  6. Dux4 induces cell cycle arrest at G1 phase through upregulation of p21 expression

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Hongliang; Wang, Zhaoxia; Jin, Suqin; Hao, Hongjun [Department of Neurology, Peking University First Hospital, Beijing 100034 (China); Zheng, Lemin [The Institute of Cardiovascular Sciences, Peking University Health Science Center, Key Laboratory of Molecular Cardiovascular Sciences of Education Ministry, Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides of Health Ministry, Beijing 100191 (China); Zhou, Boda [The Department of Cardiology, Peking University Third Hospital, Beijing 100191 (China); Zhang, Wei; Lv, He [Department of Neurology, Peking University First Hospital, Beijing 100034 (China); Yuan, Yun, E-mail: yuanyun2002@sohu.com [Department of Neurology, Peking University First Hospital, Beijing 100034 (China)

    2014-03-28

    Highlights: • Dux4 induced TE671 cell proliferation defect and G1 phase arrest. • Dux4 upregulated p21 expression without activating p53. • Silencing p21 rescued Dux4 mediated proliferation defect and cell cycle arrest. • Sp1 binding site was required for Dux4-induced p21 promoter activation. - Abstract: It has been implicated that Dux4 plays crucial roles in development of facioscapulohumeral dystrophy. But the underlying myopathic mechanisms and related down-stream events of this retrogene were far from clear. Here, we reported that overexpression of Dux4 in a cell model TE671 reduced cell proliferation rate, and increased G1 phase accumulation. We also determined the impact of Dux4 on p53/p21 signal pathway, which controls the checkpoint in cell cycle progression. Overexpression of Dux4 increased p21 mRNA and protein level, while expression of p53, phospho-p53 remained unchanged. Silencing p21 rescued Dux4 mediated proliferation defect and cell cycle arrest. Furthermore, we demonstrated that enhanced Dux4 expression increased p21 promoter activity and elevated expression of Sp1 transcription factor. Mutation of Sp1 binding site decreased dux4 induced p21 promoter activation. Chromatin immunoprecipitation (ChIP) assays confirmed the Dux4-induced binding of Sp1 to p21 promoter in vivo. These results suggest that Dux4 might induce proliferation inhibition and G1 phase arrest through upregulation of p21.

  7. SUMOylation of Rb enhances its binding with CDK2 and phosphorylation at early G1 phase.

    Science.gov (United States)

    Meng, Fengxi; Qian, Jiang; Yue, Han; Li, Xiaofeng; Xue, Kang

    2016-07-01

    Retinoblastoma protein (Rb) is a prototypical tumor suppressor that is vital to the negative regulation of the cell cycle and tumor progression. Hypo-phosphorylated Rb is associated with G0/G1 arrest by suppressing E2F transcription factor activity, whereas Rb hyper-phosphorylation allows E2F release and cell cycle progression from G0/G1 to S phase. However, the factors that regulate cyclin-dependent protein kinase (CDK)-dependent hyper-phosphorylation of Rb during the cell cycle remain obscure. In this study, we show that throughout the cell cycle, Rb is specifically small ubiquitin-like modifier (SUMO)ylated at early G1 phase. SUMOylation of Rb stimulates its phosphorylation level by recruiting a SUMO-interaction motif (SIM)-containing kinase CDK2, leading to Rb hyper-phosphorylation and E2F-1 release. In contrast, a SUMO-deficient Rb mutant results in reduced SUMOylation and phosphorylation, weakened CDK2 binding, and attenuated E2F-1 sequestration. Furthermore, we reveal that Rb SUMOylation is required for cell proliferation. Therefore, our study describes a novel mechanism that regulates Rb phosphorylation during cell cycle progression. PMID:27163259

  8. Modeling SNR G1.9+0.3 as a Supernova Inside a Planetary Nebula

    CERN Document Server

    Tsebrenko, Danny

    2014-01-01

    Using 3D numerical hydrodynamical simulations we show that a type Ia supernova (SN Ia) explosion inside a planetary nebula (PN) can explain the observed shape of the G1.9+0.3 supernova remnant (SNR), and its X-ray morphology. The SNR G1.9+0.3 morphology can be generally described as a sphere with two small and incomplete lobes protruding on opposite sides of the SNR, termed "ears", a structure resembling many elliptical PNe. Observations show the synchrotron X-ray emission to be much stronger inside the two ears than in the rest of the SNR. We numerically show that a spherical SN Ia explosion into a circumstellar matter (CSM) with the structure of an elliptical PN with ears can explain the X-ray properties of SNR G1.9+0.3. While the ejecta has already collided with the PN shell in most of the SNR and its forward shock has been slowed down, the ejecta is still advancing inside the ears. The fast forward shock inside the ears explains the stronger X-ray emission there. SN Ia inside PNe (SNIPs) seem to comprise ...

  9. Performance Factors of Cloud Computing Data Centers Using [(M/G/1 : (∞/Gdmodel] Queuing Systems

    Directory of Open Access Journals (Sweden)

    N.Ani Brown Mary

    2013-07-01

    Full Text Available The ever-increasing status of the cloud computing hypothesis and the budding concept of federated cloudcomputing have enthused research efforts towards intellectual cloud service selection aimed at developingtechniques for enabling the cloud users to gain maximum benefit from cloud computing by selectingservices which provide optimal performance at lowest possible cost. Cloud computing is a novel paradigmfor the provision of computing infrastructure, which aims to shift the location of the computinginfrastructure to the network in order to reduce the maintenance costs of hardware and software resources.Cloud computing systems vitally provide access to large pools of resources. Resources provided by cloudcomputing systems hide a great deal of services from the user through virtualization. In this paper, thecloud data center is modelled as [(M/G/1 : (/GD MODEL] queuing system with a single task arrivalsand a task request buffer of infinite capacity.

  10. Appendix E: Geology

    Energy Technology Data Exchange (ETDEWEB)

    Reidel, Steve; Chamness, Mickie A.

    2008-01-17

    This appendix provides a detailed description of geology under the Central Plateau of the Hanford Site, emphasizing the areas around tank farms. It is to be published by client CH2M HILL Hanford Group, Inc., as part of a larger, multi-contractor technical report.

  11. Panama Papers

    DEFF Research Database (Denmark)

    Harrington, Brooke

    2016-01-01

    Mossack Fonseca kept its clients largely on the right side of the law. Indeed, that’s entirely the point. This article for The Atlantic draws on my wealth management research to explain why most of what is revealed in the Panama Papers leak will not result in criminal prosecution for anyone...

  12. Collected Papers

    CERN Document Server

    Kostant, Bertram; Kumar, Shrawan; Vergne, Michele

    2009-01-01

    The author has been one of the major architects of modern Lie theory. His interests span a tremendous range of Lie theory, from differential geometry to representation theory, abstract algebra, and mathematical physics. This title features commentaries and summaries of his papers in his own words.

  13. Stabilization of IgG1 in spray-dried powders for inhalation.

    Science.gov (United States)

    Schüle, S; Schulz-Fademrecht, T; Garidel, P; Bechtold-Peters, K; Frieb, W

    2008-08-01

    The protein stabilizing capabilities of spray-dried IgG1/mannitol formulations were evaluated. The storage stability was tested at different residual moisture levels prepared by vacuum-drying or equilibration prior to storage. Vacuum-drying at 32 degrees C/0.1mbar for 24h reduced the moisture level below 1%, constituting an optimal basis for improved storage stability. The crystalline IgG1/mannitol powders with a weight ratio of 20/80 up to 40/60 failed to prevent the antibody aggregation as assessed by size exclusion chromatography during storage. Ratios of 60/40 up to 80/20 IgG1/mannitol provided superior stability of the antibody and the powders could be produced with high yields. The lower the residual moisture, the better was the stabilizing capability. An amount of 20% mannitol provided the best stabilization. Storage stability of 60/40, 70/30, and 80/20 IgG1/mannitol formulations over one year was adequate at 2-8 degrees C and 25 degrees C. Closed storage (sealed in vials) at 40 degrees C/75% RH and open storage at 25 degrees C/60% RH revealed that the stability still required optimization. The lower the protein content, the better was the powder flowability. The aerodynamic properties of powders spray-dried with 10% solids content were inadequate, as the particle size ranged between 5.1 and 7.2 microm and the fine particle fraction accounted for only 4-11%. Reduction of the solids content to 2.5% did improve the aerodynamic properties as the mass mean aerodynamic diameter was reduced to 3.6 microm and the fine particle fraction was increased to about 14%. The reduction of the solids content did not influence the storage stability significantly. Also spray-drying at higher temperatures had no significant impact on the storage stability, despite a higher tendency to form amorphous systems. In order to improve the storage stability and to maintain the good flowability of 70/30 IgG1/mannitol powder or to keep the storage stability but to improve the flowability

  14. ALDH expression characterizes G1-phase proliferating beta cells during pregnancy.

    Directory of Open Access Journals (Sweden)

    Lijuan Zhang

    Full Text Available High levels of aldehyde dehydrogenase (ALDH activity have been detected in various progenitor and stem cells. Thus, Aldefluor fluorescence, which represents precisely the ALDH activity, has been widely used for the identification, evaluation, and isolation of stem and progenitor cells. Recently, ALDH activity was detected in embryonic and adult mouse pancreas, specifically in adult centroacinar and terminal duct cells supposed to harbor endocrine and exocrine progenitor cells in the adult pancreas. Nevertheless, ALDH activity and aldeflour fluorescence have not been examined in beta cells. Here, we report a dynamic increase in the number of aldeflour+ beta cells during pregnancy. Interestingly, nearly all these aldeflour+ beta cells are positive for Ki-67, suggesting that they are in an active cell cycle (G1, S and M phases. To determine precisely at which phase beta cells activate ALDH activity and thus become aldeflour+, we co-stained insulin with additional proliferation markers, phosphohistone3 (PHH3, a marker for M-phase proliferating cells and Bromodeoxyuridine (BrdU, a marker for S-phase proliferating cells. Our data show little aldeflour+ beta cells that were positive for either PHH3, or BrdU, suggesting that beta cells activate ALDH and become Aldefluor+ when they enter G1-phase of active cell cycle, but may downregulate ALDH when they leave G1-phase and enter S phase. Our data thus reveal a potential change in ALDH activity of proliferating beta cells during pregnancy, which provides a novel method for isolation and analysis of proliferating beta cells. Moreover, our data also suggest that caution needs to be taken on interpretation of Aldefluor lineage-tracing data in pancreas.

  15. ALDH Expression Characterizes G1-Phase Proliferating Beta Cells during Pregnancy

    Science.gov (United States)

    Zhang, Lijuan; Wang, Lin; Liu, Xiaoliang; Zheng, Dongming; Liu, Sishi; Liu, Caixia

    2014-01-01

    High levels of aldehyde dehydrogenase (ALDH) activity have been detected in various progenitor and stem cells. Thus, Aldefluor fluorescence, which represents precisely the ALDH activity, has been widely used for the identification, evaluation, and isolation of stem and progenitor cells. Recently, ALDH activity was detected in embryonic and adult mouse pancreas, specifically in adult centroacinar and terminal duct cells supposed to harbor endocrine and exocrine progenitor cells in the adult pancreas. Nevertheless, ALDH activity and aldeflour fluorescence have not been examined in beta cells. Here, we report a dynamic increase in the number of aldeflour+ beta cells during pregnancy. Interestingly, nearly all these aldeflour+ beta cells are positive for Ki-67, suggesting that they are in an active cell cycle (G1, S and M phases). To determine precisely at which phase beta cells activate ALDH activity and thus become aldeflour+, we co-stained insulin with additional proliferation markers, phosphohistone3 (PHH3, a marker for M-phase proliferating cells) and Bromodeoxyuridine (BrdU, a marker for S-phase proliferating cells). Our data show little aldeflour+ beta cells that were positive for either PHH3, or BrdU, suggesting that beta cells activate ALDH and become Aldefluor+ when they enter G1-phase of active cell cycle, but may downregulate ALDH when they leave G1-phase and enter S phase. Our data thus reveal a potential change in ALDH activity of proliferating beta cells during pregnancy, which provides a novel method for isolation and analysis of proliferating beta cells. Moreover, our data also suggest that caution needs to be taken on interpretation of Aldefluor lineage-tracing data in pancreas. PMID:24787690

  16. Batch arrival M/G/1 Processor Sharing with application to Multilevel Processor Sharing scheduling

    OpenAIRE

    Avrachenkov, Konstantin; Ayesta, Urtzi; Brown, Patrick

    2003-01-01

    We analyze an M/G/1 Processor-Sharing queue with Batch arrivals. Our analysis is based on the integral equation derived by Kleinrock, Muntz and Rodemich. Using the contraction mapping principle, we demonstrate the existence and uniqueness of a solution to the integral equation. Then we provide asymptotical analysis as well as tight bounds for the expected response time conditioned on the job size. In particular, the asymptotics for large size jobs depends only on the first moment of the job s...

  17. PRODUKSI BIBIT KENTANG (Solanum tuberosum L.) G1 DARI STEK BATANG

    OpenAIRE

    Putu Wina Andriani Lestari; Made Ria Defiani; Ida Ayu Astarini

    2014-01-01

    This research was conducted to find out the best media and auxin for plantingpotato cutting to produce G1 seed potato. Research was done at a shade house ofKebun Bibit Hortikultura Kembang Merta, Candi Kuning Village, Baturiti, TabananRegency, Bali. Were planted at the screen house for sprouting. Four weeks old ofshoots were used as cutting. Each cutting measured 10 cm. Stem cutting of G0 seedpotatoes was dipped in 2 different auxin types (Rootone F and auxin paste) and plantedin 2 different ...

  18. Analysis of Repairable Geo/G/1 Queues with Negative Customers

    Directory of Open Access Journals (Sweden)

    Doo Ho Lee

    2014-01-01

    Full Text Available We consider discrete-time Geo/G/1 queues with negative customers and a repairable server. The server is subject to failure due to a negative customer arrival. As soon as a negative customer arrives at a system, the server fails and one positive (ordinary customer is forced to leave. At a failure instant, the server is turned off and the repair process immediately begins. We construct the mathematical model and present the probability generating functions of the system size distribution and the FCFS sojourn time distribution. Finally, some numerical examples are given to show the influence of negative customer arrival on the performance measures of the system.

  19. Human anti-rhesus D IgG1 antibody produced in transgenic plants

    DEFF Research Database (Denmark)

    Bouquin, Thomas; Thomsen, Mads Jonas Birkbak; Nielsen, Leif Kofoed;

    2002-01-01

    Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D...... antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent...

  20. On the M/G/1 queueing system with multiclass customers and fixed feedback

    Institute of Scientific and Technical Information of China (English)

    ZHANG Qi-zhi

    2008-01-01

    The M/G/1 queueing system with multiclass customer arrivals, fixed feedback, and first come first served policy is considered, where different classes of customers have different arrival rates, service-time distributions, and feedback numbers. The joint probability generation function of queue size of each class and the Laplace-Stieltjes transform of the total sojourn time of a customer in each class are presented, which extended the results obtained by Choi B D. The mean queue size of each class and mean total sojourn time of a customer in each class are obtained with this result. The results can be used in computer and communication networks for their performance analysis.

  1. Reservicing some customers in M/G/1 queues under three disciplines

    Directory of Open Access Journals (Sweden)

    M. R. Salehi-Rad

    2004-01-01

    Full Text Available Consider an M/G/1 production line in which a production item is failed with some probability and is then repaired. We consider three repair disciplines depending on whether the failed item is repaired immediately or first stockpiled and repaired after all customers in the main queue are served or the stockpile reaches a specified threshold. For each discipline, we find the probability generating function (p.g.f. of the steady-state size of the system at the moment of departure of the customer in the main queue, the mean busy period, and the probability of the idle period.

  2. Nonuniform Expansion of the Youngest Galactic Supernova Remnant G1.9+0.3

    Science.gov (United States)

    Reynolds, Stephen P.; Borkowski, Kazimierz J.; Green, David; Hwang, Una; Petre, Robert

    2014-08-01

    G1.9+0.3 is the youngest known Galactic supernova remnant (SNR), about 100 yr old from global expansion measurements, and most likely the result of an asymmetric Type Ia supernova explosion. We smoothed a Chandra image from a 1 Ms observation in 2011 and fit the resulting model to unsmoothed images from 2007 and 2009, allowing for expansion and image shifts. The measured expansion rates strongly deviate from uniform expansion, increasing inward by about 60% along the X-ray bright SE-NW axis, from 0.52% +- 0.03% per yr to 0.84% +- 0.06% per yr. This corresponds to undecelerated ages of 120 - 190 yr, confirming the young age of G1.9 +0.3, and implying a significant (deceleration parameter m blast wave. The spatially-integrated X-ray flux, strongly dominated by synchrotron emission, increases at a rate of 1.9% +- 0.7% per year, in agreement with previous measurements. G1.9+0.3 is the only Galactic SNR brightening at X-ray and radio wavelengths. We identify the inner rims with the reverse shock and more slowly-expanding rims farther out with the blast wave. The large spread in expansion ages between the reverse shock and the blast wave requires abrupt density gradients in either the ejecta or the ambient medium, to suddenly decelerate the reverse shock or the blast wave. The blast wave could have been decelerated recently by an encounter with a modest (factor of several) density discontinuity in the ambient medium, such as found at a wind termination shock, implying a strong presupernova wind from the progenitor system. Alternatively, the reverse shock might have encountered a larger (factor of 10 or more) density discontinuity within the SN ejecta, such as found in pulsating delayed-detonation Type Ia SN models. Through 1D hydrodynamical simulations, we demonstrate that the blast wave is much more decelerated than the reverse shock in these models for remnants at ages similar to G1.9+0.3. The presence of strong density gradients in the outer ejecta of Type Ia SNe has

  3. Asymmetric expansion of the youngest Galactic supernova remnant G1.9+0.3

    Science.gov (United States)

    Reynolds, Stephen P.

    2016-06-01

    The youngest Galactic supernova remnant (SNR) G1.9+0.3, produced by a (probable) Type Ia SN that exploded around CE 1900, is strongly asymmetric at radio wavelengths, with a single bright maximum in its shell, but exhibits a bilaterally symmetric morphology in X-rays. It has been difficult to understand the origin of these contrasting morphologies. We present the results of expansion measurements of G1.9+0.3 that illuminate the origin of the radio asymmetry. These measurements are based on a comparison of our 2015 400-ks Chandra observation with earlier Chandra observations, including a 1-Ms observation in 2011. The mean expansion rate from 2011 to 2015 is 0.58% per yr, in agreement with previous measurements. We also confirm that the expansion decreases radially away from the remnant's center along the major E-W axis, from 0.77% per yr to 0.53% per yr. Large variations in expansion are also present along the minor N-S axis, but expansion there is strongly asymmetric and varies on small spatial scales. We use the “Demons” method to study the complex motions within G1.9+0.3. This method provides a nonparametric way for measuring these motions globally. We find motions varying by a factor of 5, from 0.09" to 0.44" per year. The slowest shocks are in the north, at the outer boundary of the bright radio emission, with speeds there as low as 3,600 km/s (for an assumed distance of 8.5 kpc), much less than the average shock speed of 12,000 km/s. Such strong deceleration of the northern blast wave most likely arises from the collision of SN ejecta with a much denser than average ambient medium there. The presence of this asymmetric ambient medium naturally explains the radio asymmetry. The SN ejecta have also been strongly decelerated in the N, but they expand faster than the blast wave. In several locations, significant morphological changes and strongly nonradial motions are apparent. The spatially-integrated X-ray flux continues to increase with time. As with Kepler

  4. Modeling and Optimization of M/G/1-Type Queueing Networks: An Efficient Sensitivity Analysis Approach

    Directory of Open Access Journals (Sweden)

    Liang Tang

    2010-01-01

    Full Text Available A mathematical model for M/G/1-type queueing networks with multiple user applications and limited resources is established. The goal is to develop a dynamic distributed algorithm for this model, which supports all data traffic as efficiently as possible and makes optimally fair decisions about how to minimize the network performance cost. An online policy gradient optimization algorithm based on a single sample path is provided to avoid suffering from a “curse of dimensionality”. The asymptotic convergence properties of this algorithm are proved. Numerical examples provide valuable insights for bridging mathematical theory with engineering practice.

  5. 带启动时间的N-策略M/G/1排队系统的队长%Queue size of M/G/1 queueing system with N-policy and set-up times

    Institute of Scientific and Technical Information of China (English)

    唐应辉; 蒲会; 余玅妙

    2011-01-01

    This paper considered the M/G/1 queue with N-policy and set-up times, and directly studied both the tranaient distribution and equilibrium distribution of the queue length. By introducing the server busy period, and u8ing the total probability decomposition technique and Laplace transform, the recursion expressions of the Laplace transform of the transient queue length diatribution at any time t are obtained.Furthermore, the recursion expressions 0f the distribution and stochastic decomposition of the queue length at a random point in equilibrium are also obtained, which have important value on application. Especially,some corresponding results which have more really value in some special queueing models are obtained directly.%考虑带启动时间的N-策略M/G/1排队系统,从任意初始状态出发,直接研究了系统队长的瞬态分布和稳态分布.通过引进的"服务员忙期",使用全概率分解技术和拉普拉斯变换,导出了在任意时刻t队长的瞬态分布的拉普拉斯变换的表达式,进一步获得了有重要应用价值的稳态分布的具体的递推式子,以及稳态队长的随机分解结果.特别地,还直接获得了一些特殊排队系统的更实用的稳态队长分布的递推表达式.

  6. Bancroftian filariasis: the patterns of filarial-specific immunoglobulin G1 (IgG1), IgG4, and circulating antigens in an endemic community of northeastern Tanzania

    DEFF Research Database (Denmark)

    Simonsen, P E; Lemnge, M M; Msangeni, H A;

    1996-01-01

    The profile of filarial-specific immunoglobulin G1 (IgG1), IgG4, and Wuchereria bancrofti-specific circulating antigen (Og4C3) was analyzed in individuals one year of age and older in a community with high endemicity for Bancroftian filariasis. The overall microfilarial (mf) prevalence in the exa......The profile of filarial-specific immunoglobulin G1 (IgG1), IgG4, and Wuchereria bancrofti-specific circulating antigen (Og4C3) was analyzed in individuals one year of age and older in a community with high endemicity for Bancroftian filariasis. The overall microfilarial (mf) prevalence...

  7. Berberine inhibits growth and induces G1 arrest and apoptosis in human cholangiocarcinoma QBC939 cells.

    Science.gov (United States)

    He, Wei; Wang, Bin; Zhuang, Yun; Shao, Dong; Sun, Kewen; Chen, Jianping

    2012-01-01

    The chemotherapeutic approach using non-toxic natural products may be one of the strategies for the management of the cholangiocarcinoma. Here we report that in vitro treatment of human cholangiocarcinoma QBC939 cells with berberine, a naturally occurring isoquinoline alkaloid, decreased cell viability and induced cell death in a dose-dependent manner, which was associated with an increase in G1 arrest. Our western blot analysis showed that berberine-induced G1 cell cycle arrest was mediated through the increased expression of cyclin-dependent kinase inhibitors (Cdki) proteins (Cip1/p21 and Kip1/p27); a simultaneous decrease in Cdk2 and Cdk4 and cyclins D1, and reduced activity of the Cyclins-Cdk complex. In additional studies, treatment of QBC939 cells with different concentrations (10, 40, 80 μM) of berberine for 48 h resulted in a significant dose-dependent increase in apoptosis compared to the non-berberine-treated control, which was associated with an increased expression of pro-apoptotic protein Bax and decreased expression of anti-apoptotic proteins Bcl-2 and Bcl-xL. Together, this study for the first time identified berberine as a chemotherapeutic agent against human cholangiocarcinoma cells QBC939 cells in vitro. Further in vivo studies are required to determine whether berberine could be an effective chemotherapeutic agent for the management of cholangiocarcinoma.

  8. The extraction of the spin structure function, g2 (and g1) at low Bjorken x

    International Nuclear Information System (INIS)

    The Spin Asymmetries of the Nucleon Experiment (SANE) used the Continuous Electron Beam Accelerator Facility at Jefferson Laboratory in Newport News, VA to investigate the spin structure of the proton. The experiment measured inclusive double polarization electron asymmetries using a polarized electron beam, scattered off a solid polarized ammonia target with target polarization aligned longitudinal and near transverse to the electron beam, allowing the extraction of the spin asymmetries A1 and A2, and spin structure functions g1 and g2. Polarized electrons of energies of 4.7 and 5.9 GeV were used. The scattered electrons were detected by a novel, non-magnetic array of detectors observing a four-momentum transfer range of 2.5 to 6.5 GeV*V. This document addresses the extraction of the spin asymmetries and spin structure functions, with a focus on spin structure function, g2 (and g1) at low Bjorken x. The spin structure functions were measured as a function of x and W in four Q square bins. A full understanding of the low x region is necessary to get clean results for SANE and extend our understanding of the kinematic region at low x.

  9. Nonuniform Expansion of the Youngest Galactic Supernova Remnant G1.9+0.3

    Science.gov (United States)

    Borkowski, Kazimierz J.; Reynolds, Stephen P.; Green, David A.; Hwang, Una; Petre, Robert; Krishnamurthy, Kalyani; Willett, Rebecca

    2014-01-01

    We report measurements of the X-ray expansion of the youngest Galactic supernova remnant, G1.9+0.3, using Chandra observations in 2007, 2009, and 2011. The measured rates strongly deviate from uniform expansion, decreasing radially by about 60 along the X-ray bright SE-NW axis from 0.84 plus or minus 0.06% yr(exp -1) to 0.52% plus or minus 0.03 yr(exp -1). This corresponds to undecelerated ages of 120-190 yr, confirming the young age of G1.9+0.3 and implying a significant deceleration of the blast wave. The synchrotron-dominated X-ray emission brightens at a rate of 1.9% plus or minus 0.4% yr(exp -1). We identify bright outer and inner rims with the blast wave and reverse shock, respectively. Sharp density gradients in either the ejecta or ambient medium are required to produce the sudden deceleration of the reverse shock or the blast wave implied by the large spread in expansion ages. The blast wave could have been decelerated recently by an encounter with a modest density discontinuity in the ambient medium, such as may be found at a wind termination shock, requiring strong mass loss in the progenitor.

  10. Involvement of ATM/ATR-p38 MAPK cascade in MNNG induced G1-S arrest

    Institute of Scientific and Technical Information of China (English)

    Ke-Qing Zhu; Suo-Jiang Zhang

    2003-01-01

    AIM: To understand the effect of low concentration of Nmethyl-N′-nitro-nitrosoguanidine (MNNG), which is a widely distributed environmental mutagen and carcinogen especially for human gastric cancer, on DNA damage and to study its possible pathway in regulating cell cycle arrest.METHODS: The DNA damage effect was measured by Comet assay. A specific phospho-(Ser/Thr) ATM/ATR substrate antibody was used to detect the damage sensor by Western blot. p38 kinase activity was measured by direct kinase assay,and immunoprecipitation for the possible connection between ATM/ATR and p38 MAPK. Flow cytometry analysis and p38MAPK specific inhibitor SB203580 were combined to detect the possible cell cycle arrest by p38 MAPK.RESULTS: With the same low concentration MNNG exposure (0.2μM 2.5 h), Comet assays indicated that strand breaks accumulated, Western blot and kinase assay showed ATM/ATR and p38 kinase activated, immunoprecipitation showed phospho-ATM/ATR substrate antibody combined with both p38 MAPK antibody and phospho-p38 MAPK antibody, p38MAPK pathway was involved in the G1-S arrest.CONCLUSION: Activation of ATM/ATR by MNNG induced DNA damage leads to activation of p38 MAPK, which involves in the G1 checkpoint in mammalian cells.

  11. Permanent draft genome sequence of the gliding predator Saprospira grandis strain Sa g1 (= HR1)

    Energy Technology Data Exchange (ETDEWEB)

    Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Chertkov, Olga [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute

    2012-01-01

    Saprospira grandis Gross et al. 1911 is a member of the Saprospiraceae, a family in the class 'Sphingobacteria' that remains poorly characterized at the genomic level. The species is known for preying on other marine bacteria via 'ixotrophy'. S. grandis strain Sa g1 was isolated from decaying crab carapace in France and was selected for genome sequencing because of its isolated location in the tree of life. Only one type strain genome has been published so far from the Saprospiraceae, while the sequence of strain Sa g1 represents the second genome to be published from a non-type strain of S. grandis. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,495,250 bp long Improved-High-Quality draft of the genome with its 3,536 protein-coding and 62 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  12. Nonuniform Expansion of the Youngest Galactic Supernova Remnant G1.9+0.3

    CERN Document Server

    Borkowski, K J; Green, D A; Hwang, U; Petre, R; Krishnamurthy, K; Willett, R

    2014-01-01

    We report measurements of X-ray expansion of the youngest Galactic supernova remnant (SNR), G1.9+0.3, using Chandra observations in 2007, 2009, and 2011. The measured rates strongly deviate from uniform expansion, decreasing radially by about 60% along the X-ray bright SE-NW axis from 0."84% +/- 0."06% per yr to 0."52% +/- 0."03% per yr. This corresponds to undecelerated ages of 120-190 yr, confirming the young age of G1.9+0.3, and implying a significant deceleration of the blast wave. The spatially-integrated dominantly synchrotron X-ray flux increases at 1.9% +/- 0.4% per yr. We identify the outer and inner rims with the blast wave and reverse shock, respectively. Sudden large density gradients in either ejecta or ambient medium are required to produce the sudden deceleration of the reverse shock or the blast wave implied by the large spread in expansion ages. The blast wave could have been decelerated recently by an encounter with a modest density discontinuity in the ambient medium, such as found at a win...

  13. Engineering upper hinge improves stability and effector function of a human IgG1.

    Science.gov (United States)

    Yan, Boxu; Boyd, Daniel; Kaschak, Timothy; Tsukuda, Joni; Shen, Amy; Lin, Yuwen; Chung, Shan; Gupta, Priyanka; Kamath, Amrita; Wong, Anne; Vernes, Jean-Michel; Meng, Gloria Y; Totpal, Klara; Schaefer, Gabriele; Jiang, Guoying; Nogal, Bartek; Emery, Craig; Vanderlaan, Martin; Carter, Paul; Harris, Reed; Amanullah, Ashraf

    2012-02-17

    Upper hinge is vulnerable to radical attacks that result in breakage of the heavy-light chain linkage and cleavage of the hinge of an IgG1. To further explore mechanisms responsible for the radical induced hinge degradation, nine mutants were designed to determine the roles that the upper hinge Asp and His play in the radical reactions. The observation that none of these substitutions could inhibit the breakage of the heavy-light chain linkage suggests that the breakage may result from electron transfer from Cys(231) directly to the heavy-light chain linkage upon radical attacks, and implies a pathway separate from His(229)-mediated hinge cleavage. On the other hand, the substitution of His(229) with Tyr showed promising advantages over the native antibody and other substitutions in improving the stability and function of the IgG1. This substitution inhibited the hinge cleavage by 98% and suggests that the redox active nature of Tyr did not enable it to replicate the ability of His to facilitate radical induced degradation. We propose that the lower redox potential of Tyr, a residue that may be the ultimate sink for oxidizing equivalents in proteins, is responsible for the inhibition. More importantly, the substitution increased the antibody's binding to FcγRIII receptors by 2-3-fold, and improved ADCC activity by 2-fold, while maintaining a similar pharmacokinetic profile with respect to the wild type. Implications of these observations for antibody engineering and development are discussed.

  14. The absence of radio emission from the globular cluster G1

    CERN Document Server

    Miller-Jones, J C A; Sivakoff, G R; Heinke, C O; Miller, R E; Plotkin, R M; Di Stefano, R; Greene, J E; Ho, L C; Joseph, T D; Kong, A K H; Maccarone, T J

    2012-01-01

    The detections of both X-ray and radio emission from the cluster G1 in M31 have provided strong support for existing dynamical evidence for an intermediate mass black hole (IMBH) of mass 1.8 +/- 0.5 x 10^4 solar masses at the cluster center. However, given the relatively low significance and astrometric accuracy of the radio detection, and the non-simultaneity of the X-ray and radio measurements, this identification required further confirmation. Here we present deep, high angular resolution, strictly simultaneous X-ray and radio observations of G1. While the X-ray emission (L_X = 1.74^{+0.53}_{-0.44} x 10^{36} (d/750 kpc)^2 erg/s in the 0.5-10 keV band) remained fully consistent with previous observations, we detected no radio emission from the cluster center down to a 3-sigma upper limit of 4.7 microJy/beam. Our favored explanation for the previous radio detection is flaring activity from a black hole low mass X-ray binary (LMXB). We performed a new regression of the Fundamental Plane of black hole activity...

  15. O uso do Twitter pelos portais de notícia UOL, Terra e G1

    Directory of Open Access Journals (Sweden)

    Tellaroli, Taís Marina

    2010-01-01

    Full Text Available Como os portais de notícia G1, Portal Terra e UOL notícias usam o Twitter na publicação de notícias? Este é o ponto de partida desta pesquisa empírica envolvendo Twitter e jornalismo. Com a popularização do microblog Twitter no Brasil, procura-se compreender a maneira como os grandes portais de notícia brasileiros estão buscando a atenção do público na Internet, suas estratégias e formas de publicação de conteúdo jornalístico on-line. Para esta pesquisa foi criado um perfil no Twitter e adicionado aos seguidos os três maiores portais de notícia do Brasil: Portal Terra, UOL notícias e G1, em seguida foram coletadas 24 horas de publicações seguidas de notícias no Twitter e analisadas com o método de pesquisa Análise de Conteúdo. Para a análise foram formuladas três perguntas: Como os portais utilizam o Twitter na publicação de notícias? Qual a frequência de participação dos portais no Twitter? Quais assuntos os portais publicam no Twitter?

  16. 32 CFR Appendix D to Part 290 - Audit Working Papers

    Science.gov (United States)

    2010-07-01

    ... assignments include: (1) Internal control questionnaire. (2) Internal control review update control log. (3... normally reflect the progress of the audit and are designed to ensure continuity of the audit effort....

  17. Selected papers

    CERN Document Server

    Elgot, Calvin C

    1982-01-01

    Cal Elgot was a very serious and thoughtful researcher, who with great determi­ nation attempted to find basic explanations for certain mathematical phenomena­ as the selection of papers in this volume well illustrate. His approach was, for the most part, rather finitist and constructivist, and he was inevitably drawn to studies of the process of computation. It seems to me that his early work on decision problems relating automata and logic, starting with his thesis under Roger Lyndon and continuing with joint work with Biichi, Wright, Copi, Rutledge, Mezei, and then later with Rabin, set the stage for his attack on the theory of computation through the abstract treatment of the notion of a machine. This is also apparent in his joint work with A. Robinson reproduced here and in his joint papers with John Shepherdson. Of course in the light of subsequent work on decision problems by Biichi, Rabin, Shelah, and many, many others, the subject has been placed on a completely different plane from what it was whe...

  18. Dectin-1 agonist selectively induces IgG1 class switching by LPS-activated mouse B cells.

    Science.gov (United States)

    Seo, Beom-Seok; Park, Ha-Yan; Yoon, Hee-Kyung; Yoo, Yung-Choon; Lee, Junglim; Park, Seok-Rae

    2016-10-01

    Heat-killed Saccharomyces cerevisiae (HKSC) is an agonist for Dectin-1, a major fungal cell wall β-glucan receptor. We previously reported that HKSC selectively enhances IgG1 production by LPS-activated mouse B cells. To determine if this IgG1 selectivity is caused by selective IgG1 class switching, we performed RT-PCRs for measuring germline transcripts (GLTs), flow cytometric analyses for detecting Ig-expressing cells, and ELISPOT assays for measuring the number of Ig-secreting cells in HKSC/LPS-stimulated mouse B cell cultures. HKSC selectively enhanced expression of GLTγ1, the number of IgG1-expressing cells, and the number of IgG1-secreting B cells in the presence of LPS stimulation. In addition, HKSC induced the expression of CD69, an activation marker for B lymphocytes, and the expression of surface Dectin-1. Two Dectin-1 antagonists, laminarin and a neutralizing Dectin-1 antibody, selectively diminished HKSC-reinforced IgG1 production by LPS-stimulated B cells. Furthermore, depleted zymosan (dzn), a Dectin-1 agonist with increased selectivity, also selectively enhanced GLTγ1 transcription. The Dectin-1 antagonists blocked dzn-induced IgG1 production by LPS-activated B cells. Collectively, these results suggest that Dectin-1 agonists selectively induce IgG1 class switching by direct stimulation of Dectin-1 on LPS-activated B cells resulting in selective production of IgG1.

  19. Situation analysis of physical independence of the equipment and safety circuits of Almaraz NPP regarding R.G. 1.75 rev.3 (2005); Analisis de la situacion de la independencia fisica de los equipos y circuitos electricos de seguridad de C. N. Almaraz respecto a la R. G. 1.75 rev 3 (2005)

    Energy Technology Data Exchange (ETDEWEB)

    Seijas Portela, S.

    2010-07-01

    Situation analysis of physical independence of the electrical equipment and circuits CN safety Almaraz about R.G. 1.75 rev. 3. (2005) The aim of this paper is to present the work done in the analysis of the physical separation of redundant safety electrical equipment (emergency diesel generators, medium voltage, electrical cabinets, etc.) and physical separation of circuits and electrical conduits.

  20. Study of the extraction of residual heat for a steam generator in the presence of incondensables modeling with TRACE: PKL experiment III G1.1; Estudio de la extraccion del calor residual por un generador de vapor en presencia de incondensables modelado con TRACE: experimento PKL III G1.1

    Energy Technology Data Exchange (ETDEWEB)

    Berna, C.; Escriva, A.; Munuz-Cobo, J. L.; Romero, A.

    2012-07-01

    This paper made the simulation of the PKL III G1.1 experiment using SNAP interface and the TRACE code. This experiment aims to essentially the study of the extraction of the residual heat of the steam generator in the presence of gases incondensables.

  1. Asymmetric Expansion of the Youngest Galactic Supernova Remnant G1.9+0.3

    Science.gov (United States)

    Borkowski, Kazimierz J.; Green, David; Gwynne, Peter; Hwang, Una; Petre, Robert; Reynolds, Stephen P.; Willett, Rebecca

    2016-04-01

    The youngest Galactic supernova remnant (SNR) G1.9+0.3, produced by a (likely) Type Ia SN that exploded around CE 1900, is strongly asymmetric at radio wavelengths but exhibits a bilaterally symmetric morphology in X-rays. It has been difficult to understand the origin of these contrasting morphologies. We present results of X-ray expansion measurements of G1.9+0.3 that illuminate the origin of the radio asymmetry. These measurements are based on comparing recent (2015), 400 ks-long Chandra observations with earlier Chandra observations that include 1 Ms-long 2011 observations. The mean expansion rate from 2011 to 2015 is 0.58% yr-1, in agreement with previous measurements. We also confirm that expansion decreases radially away from the remnant's center along the major E-W axis, from 0.77% yr-1 to 0.53% yr-1. Large variations in expansion are also present along the minor N-S axis. Expansion of the faint S rim and the outermost faint N rim is comparable to the mean expansion. But the prominent X-ray rim in the N, coincident with the outer edge of the bright radio rim that marks the primary blast wave there, is expanding more slowly. Its expansion relative to the S rim is only 0.47% yr-1. At 8.5 kpc, this corresponds to a speed of about 5000 km/s, less than half of the overall blast wave speed of 12,000 km/s. Such strong deceleration of the northern blast wave most likely arises from the collision of SN ejecta with a much denser than average ambient medium there. The presence of the asymmetric ambient medium naturally explains the radio asymmetry. The SN ejecta have also been strongly decelerated in the N, but they expand faster than the blast wave. In several locations, significant morphological changes and strongly nonradial motions are apparent. The spatially-integrated X-ray flux continues to increase with time. As with Kepler's SN - the most recent historical SN in the Galaxy - the SN ejecta are likely colliding with the asymmetric circumstellar medium (CSM

  2. Birth of Archaeal Cells: Molecular Phylogenetic Analyses of G1P Dehydrogenase, G3P Dehydrogenases, and Glycerol Kinase Suggest Derived Features of Archaeal Membranes Having G1P Polar Lipids

    Science.gov (United States)

    2016-01-01

    Bacteria and Eukarya have cell membranes with sn-glycerol-3-phosphate (G3P), whereas archaeal membranes contain sn-glycerol-1-phosphate (G1P). Determining the time at which cells with either G3P-lipid membranes or G1P-lipid membranes appeared is important for understanding the early evolution of terrestrial life. To clarify this issue, we reconstructed molecular phylogenetic trees of G1PDH (G1P dehydrogenase; EgsA/AraM) which is responsible for G1P synthesis and G3PDHs (G3P dehydrogenase; GpsA and GlpA/GlpD) and glycerol kinase (GlpK) which is responsible for G3P synthesis. Together with the distribution of these protein-encoding genes among archaeal and bacterial groups, our phylogenetic analyses suggested that GlpA/GlpD in the Commonote (the last universal common ancestor of all extant life with a cellular form, Commonote commonote) acquired EgsA (G1PDH) from the archaeal common ancestor (Commonote archaea) and acquired GpsA and GlpK from a bacterial common ancestor (Commonote bacteria). In our scenario based on this study, the Commonote probably possessed a G3P-lipid membrane synthesized enzymatically, after which the archaeal lineage acquired G1PDH followed by the replacement of a G3P-lipid membrane with a G1P-lipid membrane.

  3. Optimization on bicriterion policies for M/G/1 system with second optional service

    Institute of Scientific and Technical Information of China (English)

    Jau-chuan KE; Yunn-kuang CHU

    2008-01-01

    We compare the optimal operating cost of the two bicriterion policies, p,T and p,N, for an M/G/1 queueing system with second optional service, in which the length of the vacation period is randomly controlled either by the number of arrivals during the idle period or by a timer. After all the customers are served in the queue exhaustively, the server immediately takes a vacation and may operate p, T policy or p,N policy. For the two bicriterion policies, the total average cost function per unit time is developed to search the optimal stationary operating policies at a minimum cost. Based upon the optimal cost the explicit forms for joint optimum threshold values of (p,T) and (p,N) are obtained.

  4. 游戏狙击手技嘉G1.Sniper2主板

    Institute of Scientific and Technical Information of China (English)

    马宇川

    2011-01-01

    在今年3月,技嘉科技推出了专为游戏玩家设计的G1-Killer杀手系列游戏主板产品.其最大的特点在于拥有优秀的散热与供电设计,并集成高端的创新Sound Blaster X-Fi声卡、BigFoot Killer E2100网卡,从而带给用户更好的游戏体验.该系列产品一经推出之后,就得到了高端游戏玩家的好评.

  5. Nonsinglet contributions to the structure function g$_{1}$ at small-x

    CERN Document Server

    Bartels, Julius; Ryskin, M G

    1995-01-01

    Nonsinglet contributions to the g_1(x,Q^2) structure function are calculated in the double-logarithmic approximation of perturbative QCD in the region x \\ll 1. Double logarithmic contributions of the type (\\alpha_s \\ln ^2 (1/x))^k which are not included in the GLAP evolution equations are shown to give a stronger rise at small-x than the extrapolation of the GLAP expressions. Further enhancement in the small-x region is due to non-ladder Feynman graphs which in the DLA of the unpolarized structure functions do not contribute. Compared to the conventional GLAP method (where neither the whole kinematical region which gives the double logs nor the non-ladder graphs are taken into account) our results lead to a growth at small-x which, for HERA parameters, can be larger by up to factor of 10 or more.

  6. SUBGEOMETRIC RATES OF CONVERGENCE OF THE GI/G/1 QUEUEING SYSTEM

    Institute of Scientific and Technical Information of China (English)

    Li Xiaohua; Hou Zhenting

    2012-01-01

    The article deals with the waiting time process of the GI/G/1 queueing system.We shall give that the rate of convergence to the stationary distribution and the decay of the stationary tail only depend on the tail of the service distribution,but not on the interarrival distribution.We shall also give explicit criteria for the rate of convergence and decay of stationary tail for three specific types of subgeometric cases (Case 1:the rate function r(n) =exp(sn1/1+α),α > 0,s > 0; Case 2:polynomial rate function r(n) =nα,α > 0; Case 3:logarithmic rate function r(n) =logα n,α > 0).

  7. Phytotoxic eremophilane sesquiterpenes from the coprophilous fungus Penicillium sp. G1-a14.

    Science.gov (United States)

    Del Valle, Paulina; Figueroa, Mario; Mata, Rachel

    2015-02-27

    Bioassay-directed fractionation of an extract from the grain-based culture of the coprophilous fungus Penicillium sp. G1-a14 led to the isolation of a new eremophilane-type sesquiterpene, 3R,6R-dihydroxy-9,7(11)-dien-8-oxoeremophilane (1), along with three known analogues, namely, isopetasol (2), sporogen AO-1 (3), and dihydrosporogen AO-1 (4). The structure of 1 was elucidated using 1D and 2D NMR and single-crystal X-ray diffraction. Assignment of absolute configuration at the stereogenic centers of 1 was achieved using ECD spectroscopy combined with time-dependent density functional theory calculations. Sporogen AO-1 (3) and dihydrosporogen AO-1 (4) caused significant inhibition of radicle growth against Amaranthus hypochondriacus (IC50 = 0.17 mM for both compounds) and Echinochloa crus-galli (IC50 = 0.17 and 0.30 mM, respectively). PMID:25603174

  8. Echinococcus canadensis (G7) and Echinococcus granulosus sensu stricto (G1) in swine of southern Brazil.

    Science.gov (United States)

    Monteiro, D U; Botton, S A; Tonin, A A; Azevedo, M I; Graichen, D A S; Noal, C B; de la Rue, M L

    2014-05-28

    The cystic echinococcosis (CE) is an important zoonotic disease caused by the parasite Echinococcus spp. In Brazil, this parasite is present in Rio Grande do Sul (RS) state, border with Argentina and Uruguay, causing several damages to human and animal health. This study aimed to identify Echinococcus spp. in hydatid cysts of swine and evaluate the similarity of the genotypes through the phylogenetic analysis. A total of 3,101,992 swine were slaughtered in the central/northern region of RS/Brazil, during 2008-2012. Five isolates were characterized as hydatid cyst by molecular analysis, based on the mitochondrial gene cytochrome c oxidase subunit I (cox-I). The genotypes E. granulosus sensu stricto (G1) (n=2) and E. canadensis (G7) (n=3) were identified in the hydatid cysts. The swine represents a potential intermediate host for different genotypes of Echinococcus spp., besides it can contribute to the perpetuation of the parasite's life cycle in rural areas.

  9. Human anti-rhesus D IgG1 antibody produced in transgenic plants

    DEFF Research Database (Denmark)

    Bouquin, Thomas; Thomsen, Mads; Nielsen, Leif Kofoed;

    2002-01-01

    antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent......Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D...... properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs....

  10. O uso do Twitter pelos portais de notícia UOL, Terra e G1

    OpenAIRE

    Tellaroli, Taís Marina

    2010-01-01

    Como os portais de notícia G1, Portal Terra e UOL notícias usam o Twitter na publicação de notícias? Este é o ponto de partida desta pesquisa empírica envolvendo Twitter e jornalismo. Com a popularização do microblog Twitter no Brasil, procura-se compreender a maneira como os grandes portais de notícia brasileiros estão buscando a atenção do público na Internet, suas estratégias e formas de publicação de conteúdo jornalístico on-line. Para esta pesquisa foi criado um perfil no Twitter e adici...

  11. Retinoic acid receptor agonists regulate expression of ATP-binding cassette transporter G1 in macrophages.

    Science.gov (United States)

    Ayaori, Makoto; Yakushiji, Emi; Ogura, Masatsune; Nakaya, Kazuhiro; Hisada, Tetsuya; Uto-Kondo, Harumi; Takiguchi, Shunichi; Terao, Yoshio; Sasaki, Makoto; Komatsu, Tomohiro; Iizuka, Maki; Yogo, Makiko; Uehara, Yoshinari; Kagechika, Hiroyuki; Nakanishi, Tsuyoshi; Ikewaki, Katsunori

    2012-04-01

    ABC transporter G1 (ABCG1) plays a pivotal role in HDL-mediated cholesterol efflux and atherogenesis. We investigated whether, and how, retinoic acid receptors (RARs) regulate ABCG1 expression in macrophages. All-trans retinoic acid (ATRA), an RAR ligand, increased ABCG1 protein levels and apoA-I/HDL-mediated cholesterol efflux from the macrophages. Both ATRA and other RAR agonists, TTNPB and Am580, increased major transcripts driven by promoter B upstream of exon 5, though minor transcripts driven by promoter A upstream of exon 1 were only increased by ATRA. The stimulatory effects of ATRA on ABCG1 expression were completely abolished in the presence of RAR/RXR antagonists but were only partially canceled in the presence of an LXR antagonist. Adenovirus with overexpressed oxysterol sulfotransferase abolished the LXR pathway, as previously reported, and ATRA-responsiveness in ABCA1/ABCG1 expressions were respectively attenuated by 38 and 22% compared to the control virus. Promoter assays revealed that ABCG1 levels were regulated more by promoter B than promoter A, and ATRA activated promoter B in a liver X receptor-responsive element (LXRE)-dependent manner. Further, LXRE-B in intron 7, but not LXRE-A in intron 5, enhanced ATRA responsiveness under overexpression of all RAR isoforms-RARα/β/γ. In contrast, the activation of promoter B by TTNPB depended on LXRE-B and RARα, but not on RARβ/γ. Finally, chromatin immunoprecipitation and gel-shift assays revealed a specific and direct repeat 4-dependent binding of RARα to LXRE-B. In conclusion, RAR ligands increase ABCA1/G1 expression and apoA-I/HDL-mediated cholesterol efflux from macrophages, and modulate ABCG1 promoter activity via LXRE-dependent mechanisms.

  12. Induction of apoptosis by homoharringtonine in G1 phase human chronic myeloid leukemic cells

    Institute of Scientific and Technical Information of China (English)

    MAI Wen-yuan; LIN Mao-fang

    2005-01-01

    Background Homoharringtonine (HHT) is a cephalotaxine ester derived from an evergreen tree found wildely throughout southern China, which has antileukemic activities against a variety of acute myeloid leukemic cells. For the sake of illustrating the mechanisms of HHT in the treatment of leukemia, we assessed the effect of HHT on the apoptosis of human chronic myeloid leukemic cell line K562.Methods The apoptosis of K562 cells induced by HHT was analyzed by transmission electron microscopy, agarose gel electrophoresis of DNA, flow cytometry and terminal deoxyribonucleotidyl transferase-mediated dUTP-biotin nick labeling.Results Characteristic apoptosis-related features emerged in K562 cells after exposed to HHT at a concentration 0.05-100 μg/ml. Transmission electron microscopy of HHT treated K562 cells displayed chromatin condensation and aggregation under the nuclear membrane, nuclear fragmentation and apoptosis body formation. Typical DNA ladder in agarose gel electrophoresis was observed in the cells exposed to HHT. The cell cycle analysis measured by flow cytometry showed G1 phase cells decreased with the increase of S phase cells while apoptosis was induced by HHT in K562 cells. The percentage of apoptotic cells in K562 cells treated with 50 μg/ml of HHT decreased significantly when pretreated with 1 μg/ml of cycloheximide, 0.05 μg/ml of Actinomycin D respectively.Conclusions HHT has apoptotic effects on K562 cells. The HHT induced apoptosis mainly of the cells in G1 phase and this process required RNA transcription and protein synthesis.

  13. Preliminary stratigraphic and petrologic characterization of core samples from USW-G1, Yucca Mountain, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Waters, A.C.; Carroll, P.R. (eds.)

    1981-11-01

    Tuffs of the Nevada Test Site are currently under investigation to determine their potential for long-term storage of radioactive waste. As part of this program, hole USW-G1 was drilled to a depth of 6000 ft below the surface, in the central part of the Yucca Mountain area, Nevada Test Site, Nevada. Petrographic study of the USW-G1 core is presented in this report and shows the tuffs (which generally were variably welded ash flows) are partly recrystallized to a variety of secondary minerals. The important alteration products are zeolites (heulandite, clinoptilolite, mordenite and analcime), smectite clays with minor interstratified illite, albite, micas, potassium feldspar, and various forms of silica. Iijima`s zeolite zones I through IV of burial metamorphism can be recognized in the core. Zeolites are first observed at about the 1300-ft depth, and the high-temperature boundary of zeolite stability in this core occurs at about 4350 ft. Analcime persists, either metastably or as a retrograde mineral, deeper in the core. The oxidation state of Fe-Ti oxide minerals, through most of the core, increases as the degree of welding decreases, but towards the bottom of the hole, reducing conditions generally prevail. Four stratigraphic units transected by the core may be potentially favorable sites for a waste repository. These four units, in order of increasing depth in the core, are (1) the lower cooling unit of the Topopah Spring Member, (2) cooling unit II of the Bullfrog Member, (3) the upper part of the Tram tuff, and (4) the Lithic-rich tuff.

  14. The impact of geoengineering on vegetation in experiment G1 of the GeoMIP

    Science.gov (United States)

    Glienke, Susanne; Irvine, Peter J.; Lawrence, Mark G.

    2015-10-01

    Solar Radiation Management (SRM) has been proposed as a mean to partly counteract global warming. The Geoengineering Model Intercomparison Project (GeoMIP) has simulated the climate consequences of a number of SRM techniques. Thus far, the effects on vegetation have not yet been thoroughly analyzed. Here the vegetation response to the idealized GeoMIP G1 experiment from eight fully coupled Earth system models (ESMs) is analyzed, in which a reduction of the solar constant counterbalances the radiative effects of quadrupled atmospheric CO2 concentrations (abrupt4 × CO2). For most models and regions, changes in net primary productivity (NPP) are dominated by the increase in CO2, via the CO2 fertilization effect. As SRM will reduce temperatures relative to abrupt4 × CO2, in high latitudes this will offset increases in NPP. In low latitudes, this cooling relative to the abrupt4 × CO2 simulation decreases plant respiration while having little effect on gross primary productivity, thus increasing NPP. In Central America and the Mediterranean, generally dry regions which are expected to experience increased water stress with global warming, NPP is highest in the G1 experiment for all models due to the easing of water limitations from increased water use efficiency at high-CO2 concentrations and the reduced evaporative demand in a geoengineered climate. The largest differences in the vegetation response are between models with and without a nitrogen cycle, with a much smaller CO2 fertilization effect for the former. These results suggest that until key vegetation processes are integrated into ESM predictions, the vegetation response to SRM will remain highly uncertain.

  15. Preliminary stratigraphic and petrologic characterization of core samples from USW-G1, Yucca Mountain, Nevada

    International Nuclear Information System (INIS)

    Tuffs of the Nevada Test Site are currently under investigation to determine their potential for long-term storage of radioactive waste. As part of this program, hole USW-G1 was drilled to a depth of 6000 ft below the surface, in the central part of the Yucca Mountain area, Nevada Test Site, Nevada. Petrographic study of the USW-G1 core is presented in this report and shows the tuffs (which generally were variably welded ash flows) are partly recrystallized to a variety of secondary minerals. The important alteration products are zeolites (heulandite, clinoptilolite, mordenite and analcime), smectite clays with minor interstratified illite, albite, micas, potassium feldspar, and various forms of silica. Iijima's zeolite zones I through IV of burial metamorphism can be recognized in the core. Zeolites are first observed at about the 1300-ft depth, and the high-temperature boundary of zeolite stability in this core occurs at about 4350 ft. Analcime persists, either metastably or as a retrograde mineral, deeper in the core. The oxidation state of Fe-Ti oxide minerals, through most of the core, increases as the degree of welding decreases, but towards the bottom of the hole, reducing conditions generally prevail. Four stratigraphic units transected by the core may be potentially favorable sites for a waste repository. These four units, in order of increasing depth in the core, are (1) the lower cooling unit of the Topopah Spring Member, (2) cooling unit II of the Bullfrog Member, (3) the upper part of the Tram tuff, and (4) the Lithic-rich tuff

  16. Nuclear vasohibin-2 promotes cell proliferation by inducing G0/G1 to S phase progression.

    Science.gov (United States)

    Ge, Qianqian; Zhou, Jia; Tu, Min; Xue, Xiaofeng; Li, Zhanjun; Lu, Zipeng; Wei, Jishu; Song, Guoxin; Chen, Jianmin; Guo, Feng; Jiang, Kuirong; Miao, Yi; Gao, Wentao

    2015-09-01

    As a member of the vasohibin (VASH2) family, VASH2 is localized intracellularly as a nuclear and cytoplasmic type. Cytoplasmic VASH2 is associated with carcinoma angiogenesis and malignant transformation and promotes cancer growth. However, the function of nuclear VASH2 has yet to be investigated. The aim of the present study was to detect the nuclear VASH2 expression profile in human organs and tissues by protein microarray technique. To examine the function of nuclear VASH2, we analyzed the relationship between nuclear VASH2 and Ki-67, and stably constructed VASH2 overexpression and knockdown in LO2 and HepG2 cell lines, based on a previous study in hepatic cells. The study was conducted using bromodeoxyuridine, immunofluorescent staining, western blot analysis and flow cytometry. Nuclear VASH2 was highly expressed in actively dividing cells in normal and cancer tissues. There was a significant positive correlation between nuclear VASH2 and Ki-67, indicating that nuclear VASH2 positively correlated with cell proliferation in normal and cancer tissues. The bromodeoxyuridine (BrdU) proliferation test showed that nuclear VASH2 increased the S-phase population and promoted cell proliferation, while VASH2 knockdown reduced BrdU absorbance. Cell cycle analysis revealed that nuclear VASH2 overexpression increased the S-phase population in LO2 and HepG2 cells, while nuclear VASH2 knockdown reduced the S-phase population and increased the G0/G1 population. The findings of this study challenge the classic view of VASH2, which was previously reported as an angiogenesis factor. Furthermore, to the best of our knowledge, these results are the first clinical data indicating that nuclear VASH2, but not cytoplasmic VASH2, promotes cell proliferation by driving the cell cycle from the G0/G1 to S phase.

  17. Supernova Ejecta in the Youngest Galactic Supernova Remnant G1.9+0.3

    Science.gov (United States)

    Borkowski, Kazimierz J.; Reynolds, Stephen P.; Hwang, Una; Green, David A.; Petre, Robert; Krishnamurthy, Kalyani; Willett, Rebecca

    2013-01-01

    G1.9+0.3 is the youngest known Galactic supernova remnant (SNR), with an estimated supernova (SN) explosion date of approximately 1900, and most likely located near the Galactic Center. Only the outermost ejecta layers with free-expansion velocities (is) approximately greater than 18,000 km s-1 have been shocked so far in this dynamically young, likely Type Ia SNR. A long (980 ks) Chandra observation in 2011 allowed spatially-resolved spectroscopy of heavy-element ejecta. We denoised Chandra data with the spatio-spectral method of Krishnamurthy et al., and used a wavelet based technique to spatially localize thermal emission produced by intermediate-mass elements (IMEs: Si and S) and iron. The spatial distribution of both IMEs and Fe is extremely asymmetric, with the strongest ejecta emission in the northern rim. Fe K alpha emission is particularly prominent there, and fits with thermal models indicate strongly oversolar Fe abundances. In a localized, outlying region in the northern rim, IMEs are less abundant than Fe, indicating that undiluted Fe-group elements (including 56Ni) with velocities greater than 18,000 km s-1 were ejected by this SN. But in the inner west rim, we find Si- and S-rich ejecta without any traces of Fe, so high-velocity products of O-burning were also ejected. G1.9+0.3 appears similar to energetic Type Ia SNe such as SN 2010jn where iron-group elements at such high free-expansion velocities have been recently detected. The pronounced asymmetry in the ejecta distribution and abundance inhomogeneities are best explained by a strongly asymmetric SN explosion, similar to those produced in some recent 3D delayed-detonation Type Ia models.

  18. A prominent lack of IgG1-Fc fucosylation of platelet alloantibodies in pregnancy.

    Science.gov (United States)

    Kapur, Rick; Kustiawan, Iwan; Vestrheim, Anne; Koeleman, Carolien A M; Visser, Remco; Einarsdottir, Helga K; Porcelijn, Leendert; Jackson, Dave; Kumpel, Belinda; Deelder, André M; Blank, Dennis; Skogen, Björn; Killie, Mette Kjaer; Michaelsen, Terje E; de Haas, Masja; Rispens, Theo; van der Schoot, C Ellen; Wuhrer, Manfred; Vidarsson, Gestur

    2014-01-23

    Immunoglobulin G (IgG) formed during pregnancy against human platelet antigens (HPAs) of the fetus mediates fetal or neonatal alloimmune thrombocytopenia (FNAIT). Because antibody titer or isotype does not strictly correlate with disease severity, we investigated by mass spectrometry variations in the glycosylation at Asn297 in the IgG Fc because the composition of this glycan can be highly variable, affecting binding to phagocyte IgG-Fc receptors (FcγR). We found markedly decreased levels of core fucosylation of anti-HPA-1a-specific IgG1 from FNAIT patients (n = 48), but not in total serum IgG1. Antibodies with a low amount of fucose displayed higher binding affinity to FcγRIIIa and FcγRIIIb, but not to FcγRIIa, compared with antibodies with a high amount of Fc fucose. Consequently, these antibodies with a low amount of Fc fucose showed enhanced phagocytosis of platelets using FcγRIIIb(+) polymorphonuclear cells or FcγRIIIa(+) monocytes as effector cells, but not with FcγRIIIa(-) monocytes. In addition, the degree of anti-HPA-1a fucosylation correlated positively with the neonatal platelet counts in FNAIT, and negatively to the clinical disease severity. In contrast to the FNAIT patients, no changes in core fucosylation were observed for anti-HLA antibodies in refractory thrombocytopenia (post platelet transfusion), indicating that the level of fucosylation may be antigen dependent and/or related to the immune milieu defined by pregnancy.

  19. Cost-effectiveness of antiviral therapy in chronic hepatitis C (G1

    Directory of Open Access Journals (Sweden)

    A. V. Rudakova

    2015-01-01

    Full Text Available Updated HCV clinical guidelines placed direct acting agents (DAAs as the preferable the first line regimens.The objective of the study was PE assessment of HCV therapy among G1 naïve patientsMethods: Analysis is based on data of randomized clinical trials and average price of HCV medicines from state auctions placed in state procurement system in 2015.Results: PTV/OBV/DSV/r cost is 30,5% lower vs PegIFN/RBV/SMV. In comparison with PegIFN/RBV/BCV combination PTV/OBV/DSV/r is cost saving by 10,6% at patients without cirrhosis and 36,2% at patients with cirrhosis. DCV/ASV combination is chipper PTV/OBV/DSV/r and it would be used for G1 naïve patient (cost saving is 9,4-10,4%. DCV/ASV and PTV/OBV/DSV/r SVR12 costs are comparable and significantly lower than PegIFN-based regimen: PegIFN/RBV/SMV and PegIFN/RBV/BCV. 4 weeks stop rules due to therapy inefficiency for PegIFN/RBV/SMV regimen could cut cost by 12,6% и 28,0% among patients without and cirrhosis accordingly. By way PTV/OBV/DSV/r is the most cost effective versus PegIFN/RBV/SMV. PTV/OBV/DSV/r as the first line therapy for PegIFN experienced patients provides budget saving 118,2 thousand RUB or 12,2% of budget.Conclusion: Right now PTV/OBV/DSV/r regimen is the most cost effective the first line therapy for naïve patients.

  20. Distinct Initiation and Maintenance Mechanisms Cooperate to Induce G1 Cell Cycle Arrest in Response to DNA Damage

    NARCIS (Netherlands)

    Agami, R.; Bernards, R.A.

    2000-01-01

    DNA damage causes stabilization of p53, leading to G1 arrest through induction of p21cip1. As this process requires transcription, several hours are needed to exert this response. We show that DNA damage causes an immediate and p53-independent G1 arrest, caused by rapid proteolysis of cyclin D1. Deg

  1. Phosphorylation-triggered CUEDC2 degradation promotes UV-induced G1 arrest through APC/C(Cdh1) regulation.

    Science.gov (United States)

    Zhang, Wei-Na; Zhou, Jie; Zhou, Tao; Li, Ai-Ling; Wang, Na; Xu, Jin-Jing; Chang, Yan; Man, Jiang-Hong; Pan, Xin; Li, Tao; Li, Wei-Hua; Mu, Rui; Liang, Bing; Chen, Liang; Jin, Bao-Feng; Xia, Qing; Gong, Wei-Li; Zhang, Xue-Min; Wang, Li; Li, Hui-Yan

    2013-07-01

    DNA damage triggers cell cycle arrest to provide a time window for DNA repair. Failure of arrest could lead to genomic instability and tumorigenesis. DNA damage-induced G1 arrest is generally achieved by the accumulation of Cyclin-dependent kinase inhibitor 1 (p21). However, p21 is degraded and does not play a role in UV-induced G1 arrest. The mechanism of UV-induced G1 arrest thus remains elusive. Here, we have identified a critical role for CUE domain-containing protein 2 (CUEDC2) in this process. CUEDC2 binds to and inhibits anaphase-promoting complex/cyclosome-Cdh1 (APC/C(Cdh1)), a critical ubiquitin ligase in G1 phase, thereby stabilizing Cyclin A and promoting G1-S transition. In response to UV irradiation, CUEDC2 undergoes ERK1/2-dependent phosphorylation and ubiquitin-dependent degradation, leading to APC/C(Cdh1)-mediated Cyclin A destruction, Cyclin-dependent kinase 2 inactivation, and G1 arrest. A nonphosphorylatable CUEDC2 mutant is resistant to UV-induced degradation. Expression of this stable mutant effectively overrides UV-induced G1-S block. These results establish CUEDC2 as an APC/C(Cdh1) inhibitor and indicate that regulated CUEDC2 degradation is critical for UV-induced G1 arrest.

  2. Antitumor Efficacy of Anti-GD2 IgG1 Is Enhanced by Fc Glyco-Engineering.

    Science.gov (United States)

    Xu, Hong; Guo, Hongfen; Cheung, Irene Y; Cheung, Nai-Kong V

    2016-07-01

    The affinity of therapeutic antibodies for Fcγ receptors (FcγRs) strongly influences their antitumor potency. To generate antibodies with optimal binding and immunologic efficacy, we compared the affinities of different versions of an IgG1 Fc region that had an altered peptide backbone, altered glycans, or both. To produce IgG1 with glycans that lacked α1,6-fucose, we used CHO cells that were deficient in the enzyme UDP-N-acetylglucosamine: α-3-d-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT1), encoded by the MGAT1 gene. Mature N-linked glycans require this enzyme, and without it, CHO cells synthesize antibodies carrying only Man5-GlcNAc2, which were more effective in antibody-dependent cell-mediated cytotoxicity (ADCC). Our engineered IgG1, hu3F8-IgG1, is specific for GD2, a neuroendocrine tumor ganglioside. Its peptide mutant is IgG1-DEL (S239D/I332E/A330L), both produced in wild-type CHO cells. When produced in GnT1-deficient CHO cells, we refer to them as IgG1n and IgG1n-DEL, respectively. Affinities for human FcγRs were measured using Biacore T-100 (on CD16 and CD32 polymorphic alleles), their immunologic properties compared for ADCC and complement-mediated cytotoxicity (CMC) in vitro, and pharmacokinetics and antitumor effects were compared in vivo in humanized mice. IgG1n and IgG1n-DEL contained only mannose and acetylglucosamine and had preferential affinity for activating CD16s, over inhibitory CD32B, receptors. In vivo, the antitumor effects of IgG1, IgG1-DEL, and IgG1n-DEL were similar but modest, whereas IgG1n was significantly more effective (P < 0.05). Thus, IgG1n antibodies produced in GnT1-deficient CHO cells may have potential as improved anticancer therapeutics. Cancer Immunol Res; 4(7); 631-8. ©2016 AACR. PMID:27197064

  3. RELATIONSHIP BETWEEN CYCLIN G1 AND HUMAN PAPILLOMA VIRUS INFECTION IN CERVICAL INTRAEPITHELIAL NEOPLASIA AND CERVICAL CARCINOMA

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Objective To evaluate the overexpression of cyclin G1 in cervical intraepithelial neoplasia (CIN) and cervical carcinoma, and the correlation between cyclin G1 and high-risk human papilloma virus (HPV) infection.Methods All of the specimens were obtained from the Department of Pathology of China-Japan Friendship Hospital from January 2000 to August 2004. We detected the expression of cyclin G1 with immunohistochemistry, HPV16/18infection with in situ hybridization, and high-risk HPV infection with Hybrid capture system Ⅱ (HC-Ⅱ) in normal group (25 cases), CIN Ⅰ (48 cases), CIN Ⅱ (56 cases), CIN Ⅲ (54 cases), and invasive cervical squamous-cell carcinoma (SCC, 31 cases).Results The positive rates of cyclin G1 expression in CIN (77. 85%) and SCC cervical tissues (87.10%) were significantly higher than normal (8.00%,P<0.01), and the intensities of cyclin G1 expression in CIN (40.60%)and SCC cervical tissues (61.51%) were significantly higher than normal (2.72%,P<0.05). The positive rates and intensities of cyclin G1 expression increased gradually with the grade of cervical lesions. High-risk HPV infection rates were higher in CIN and SCC than normal groups (P<0.05). There was a positive correlation between cyclin G1 expression and high-risk HPV infection detected with HC-Ⅱ (Kendall's tau-b =0.316, 0.269, 0.352, and 0. 474 in CIN Ⅰ, CINⅡ, CIN Ⅲ, and SCC, respectively, P<0.05).Conclusions Cyclin G1 is overexpressed in CIN and SCC. Cyclin G1 may be a biomarker for detecting CIN and SCC. Cyclin G1 may play an important role in the oncogenesis of CIN and SCC by high-risk HPV infection.

  4. G1/ELE Functions in the Development of Rice Lemmas in Addition to Determining Identities of Empty Glumes.

    Science.gov (United States)

    Liu, Mengjia; Li, Haifeng; Su, Yali; Li, Wenqiang; Shi, Chunhai

    2016-01-01

    Rice empty glumes, also named sterile lemmas or rudimentary lemmas according to different interpretations, are distinct from lemmas in morphology and cellular pattern. Consistently, the molecular mechanism to control the development of lemmas is different from that of empty glumes. Rice LEAFY HULL STERILE1(OsLHS1) and DROOPING LEAF(DL) regulate the cellular pattern and the number of vascular bundles of lemmas respectively, while LONG STERILE LEMMA1 (G1)/ELONGATED EMPTY GLUME (ELE) and PANICLE PHYTOMER2 (PAP2)/OsMADS34 determine identities of empty glumes. Though some progress has been made, identities of empty glumes remain unclear, and genetic interactions between lemma genes and glume genes have been rarely elucidated. In this research, a new G1/ELE mutant g1-6 was identified and the phenotype was analyzed. Similar to previously reported mutant lines of G1/ELE, empty glumes of g1-6 plants transform into lemma-like organs. Furthermore, Phenotypes of single and double mutant plants suggest that, in addition to their previously described gene-specific functions, G1/ELE and OsLHS1 play redundant roles in controlling vascular bundle number, cell volume, and cell layer number of empty glumes and lemmas. Meanwhile, expression patterns of G1/ELE in osmads1-z flowers and OsLHS1 in g1-6 flowers indicate they do not regulate each other at the level of transcription. Finally, down-regulation of the empty glume gene OsMADS34/PAP2 and ectopic expression of the lemma gene DL, in the g1-6 plants provide further evidence that empty glumes are sterile lemmas. Generally, our findings provided valuable information for better understanding functions of G1 and OsLHS1 in flower development and identities of empty glumes. PMID:27462334

  5. Scientific research attitude and paper writing

    International Nuclear Information System (INIS)

    This book introduces meanings and kinds of paper, structures and characteristics of paper, preparation of writing paper, writing paper, comments and footnotes, how to collect materials, and list of reference and appendix. It explains composition of chapters and paragraphs and development of contents, how to use library materials, writing draft, usage of quotations and comments, mathematical tables and charts, completing draft, basic principle of footnotes and how to fill up them, survey method, survey design, experimental methods, and real examples of comments and references.

  6. Heavy-Element Ejecta in G1.9+0.3

    Science.gov (United States)

    Borkowski, Kazimierz J.; Reynolds, S. P.; Green, D.; Hwang, U.; Petre, R.; Krishnamurthy, K.; Willett, R.

    2013-04-01

    G1.9+0.3 is the youngest known Galactic supernova remnant (SNR), with an estimated supernova (SN) explosion date of about 1900, most likely located near the Galactic center. Only the outermost ejecta layers with free-expansion velocities in excess of 18,000 km/s have been shocked so far in this dynamically-young, likely Type Ia SNR. A long (980 ks) Chandra observation in 2011 allowed for spatially-resolved spectroscopy of heavy-element ejecta. We denoised Chandra data with the spatio-spectral method of Krishnamurthy, Raginsky, & Willett, and then used a wavelet-based technique to spatially localize thermal emission produced by intermediate-mass elements (IMEs: Si, S, Ar, and Ca) and by iron. The spatial distribution of both IMEs and Fe is extremely asymmetric and inhomogeneous, with the strongest ejecta emission in the northern limb. Fe K emission is particularly prominent there, and fits with a thermal plane-shock model indicate strongly oversolar Fe abundances. In a localized, outlying region in the northern shell, IMEs are at least 5 times less abundant than Fe (by mass), indicating that undiluted Fe-group elements (including radioactive Ni) with velocities > 18,000 km/s were ejected by this SN. More modest (up to a factor of 2) Fe overabundances with respect to IMEs are present in other locations within the northern limb. There are several thousandths of a solar mass of shocked Fe in G1.9+0.3. In several locations within the remnant, including the (inner) west limb, we also find Si- and S-rich ejecta without any traces of Fe, so high-velocity, presumably undiluted products of O-burning were also ejected by the SN. If the underlying continuum is thermal, with plasma temperatures of 3-4 keV, then it must be produced by lighter elements such as O that comprise the bulk of the shocked gas. We discuss these findings in the context of Type Ia SNe such as SN 2010jn where iron-group elements at such high free-expansion velocities have been recently detected. We also

  7. Emergence and Characterization of Unusual DS-1-Like G1P[8] Rotavirus Strains in Children with Diarrhea in Thailand.

    Directory of Open Access Journals (Sweden)

    Satoshi Komoto

    Full Text Available The emergence and rapid spread of unusual DS-1-like G1P[8] rotaviruses in Japan have been recently reported. During rotavirus surveillance in Thailand, three DS-1-like G1P[8] strains (RVA/Human-wt/THA/PCB-180/2013/G1P[8], RVA/Human-wt/THA/SKT-109/2013/G1P[8], and RVA/Human-wt/THA/SSKT-41/2013/G1P[8] were identified in stool specimens from hospitalized children with severe diarrhea. In this study, we sequenced and characterized the complete genomes of strains PCB-180, SKT-109, and SSKT-41. On whole genomic analysis, all three strains exhibited a unique genotype constellation including both genogroup 1 and 2 genes: G1-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. This novel genotype constellation is shared with Japanese DS-1-like G1P[8] strains. Phylogenetic analysis revealed that the G/P genes of strains PCB-180, SKT-109, and SSKT-41 appeared to have originated from human Wa-like G1P[8] strains. On the other hand, the non-G/P genes of the three strains were assumed to have originated from human DS-1-like strains. Thus, strains PCB-180, SKT-109, and SSKT-41 appeared to be derived through reassortment event(s between Wa-like G1P[8] and DS-1-like human rotaviruses. Furthermore, strains PCB-180, SKT-109, and SSKT-41 were found to have the 11-segment genome almost indistinguishable from one another in their nucleotide sequences and phylogenetic lineages, indicating the derivation of the three strains from a common origin. Moreover, all the 11 genes of the three strains were closely related to those of Japanese DS-1-like G1P[8] strains. Therefore, DS-1-like G1P[8] strains that have emerged in Thailand and Japan were assumed to have originated from a recent common ancestor. To our knowledge, this is the first report on whole genome-based characterization of DS-1-like G1P[8] strains that have emerged in an area other than Japan. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G1P[8] rotaviruses.

  8. Radioactive Scandium in the Youngest Galactic Supernova Remnant G1.9+0.3

    CERN Document Server

    Borkowski, Kazimierz J; Green, David A; Hwang, Una; Petre, Robert; Krishnamurthy, Kalyani; Willett, Rebecca

    2010-01-01

    We report the discovery of thermal X-ray emission from the youngest Galactic supernova remnant (SNR) G1.9+0.3, from a 237-ks Chandra observation. We detect strong K-shell lines of Si, S, Ar, Ca, and Fe. In addition, we detect a 4.1 keV line with 99.971% confidence which we attribute to 44Sc, produced by electron capture from 44Ti. Combining the data with our earlier Chandra observation allows us to detect the line in two regions independently. For a remnant age of 100 yr, our measured total line strength indicates synthesis of $(1 - 7) \\times 10^{-5}$ solar masses of 44Ti, in the range predicted for both Type Ia and core-collapse (CC) supernovae, but somewhat smaller than the $2 \\times 10^{-4}$ solar masses reported for Cas A. The line spectrum indicates supersolar abundances. The Fe emission has a width of about 26,000 km/s, consistent with an age of about 100 yr and with the inferred mean shock velocity of 14,000 km/s deduced assuming a distance of 8.5 kpc. Most thermal emission comes from regions of lower ...

  9. A case of neuroendocrine tumor G1 with unique histopathological growth progress

    Institute of Scientific and Technical Information of China (English)

    Misuzu; Hirai; Kenshi; Matsumoto; Hiroya; Ueyama; Hirohumi; Fukushima; Takashi; Murakami; Hitoshi; Sasaki; Akihito; Nagahara; Takashi; Yao; Sumio; Watanabe

    2013-01-01

    A gastric neuroendocrine tumor(NET)is generated from deep within the tissue mucosal layers.In many cases,NETs are discovered as submucosal tumor(SMT)-like structures by forming a tumor mass.This case has a clear mucosal demarcation line and developed like a polyp.A dilated blood vessel was found on the surface.The mass lacked the yellow color characteristic of NETs,and a SMT-like form was evident.Therefore,a nonspecific epithelial lesion was suspected and we performed endoscopy with magnifying narrowband imaging(M-NBI).However,this approach did not lead to the diagnosis,as we diagnosed the lesion as a NET by biopsy examination.The lesion was excised by endoscopic submucosal dissection.The histopathological examination proved that the lesion was a polypoid lesion although it was also a NET because the tumorcells extended upward through the normal gland ducts scatteredly.To our knowledge,there is no previous report of NET G1 with such unique histopathological growth progress and macroscopic appearance shown by detailed examination using endoscopy with M-NBI.

  10. Bioreducible cross-linked polymers based on G1 peptide dendrimer as potential gene delivery vectors.

    Science.gov (United States)

    Li, Chun-Yan; Wang, Hai-Jiao; Cao, Jing-Ming; Zhang, Ji; Yu, Xiao-Qi

    2014-11-24

    A series of cationic polymers based on low generation (G1) peptide dendrimer were synthesized with disulfide-containing linkages. The DNA binding abilities of the target polymers were studied by gel electrophoresis and fluorescence quenching assay. The bioreducible property of the disulfide-containing polymers P2 and P3 was also investigated in the presence of dithiothreitol (DTT). Results from dynamic light scattering (DLS) and transmission electron microscopy (TEM) assays reveal that these materials may condense DNA into nanoparticles with proper sizes and zeta-potentials. In vitro cell experiments show that compared to branched 25 KDa PEI, P2 and P3 may exhibit much higher gene transfection efficiency and lower cytotoxicity in both HEK293 and U-2OS cells. Additionally, polymer prepared from Michael addition gives better gene transfection ability, while polymer prepared from ring-opening reaction has better serum tolerance. Results indicate that these polymers might be promising non-viral gene vectors for their easy preparation, very low cytotoxicity, and good transfection efficiency. PMID:25282264

  11. First passage times in M2[X ]|G |1 |R queue with hysteretic overload control policy

    Science.gov (United States)

    Pechinkin, Alexander V.; Razumchik, Rostislav R.; Zaryadov, Ivan S.

    2016-06-01

    One of the reported approaches towards the solution of overload problem in networks of SIP servers is the implementation of multi-level hysteretic control of arrivals in SIP servers. Each level, being the parameter of the policy, specifies operation mode of SIP server i.e. it implicitly indicates what SIP server must do with the arriving packets. The choice of parameters' values is not guided by standards and is usually left for the network owner. In general, all operation modes of the considered policy can be grouped into two groups: normal mode (when all arriving packets are accepted) and congested mode (when part or all arriving packets are being dropped). Such grouping may serve as the criteria for choosing parameters' values of the policy: pick those values which minimize SIP server sojourn time in congested mode. In this short note we propose some analytical results which facilitate the solution of stated minimization problem. The considered mathematical model of SIP server is the queueing system M2[X ]|G |1 |R with batch arrivals and bi-level hysteretic control policy, which specifies three operation modes: normal (customers both flows are accepted), overload (only customers from one flow are accepted), discard (customers from both flows are blocked/lost)). The switching between modes can occur only on service completions. Analytical method allowing computation of stationary sojourn times in different operation modes (as well as first passage times between modes) is presented in brief. Numerical example is given.

  12. Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1.

    Science.gov (United States)

    Dewitte, Griet; Walmagh, Maarten; Diricks, Margo; Lepak, Alexander; Gutmann, Alexander; Nidetzky, Bernd; Desmet, Tom

    2016-09-10

    UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their potential application in glycosylation processes. Recombinant expression in E. coli provided sufficient amounts of enzyme for the in-depth characterization of the salicylic acid UGT from Capsella rubella (UGT-SACr) and the stevia UGT from Stevia rebaudiana (UGT-76G1Sr). The latter was found to have a remarkably broad specificity with activities on a wide diversity of structures, from aliphatic and branched alcohols, over small phenolics to larger flavonoids, terpenoids and even higher glycoside compounds. As an example for its industrial potential, the glycosylation of curcumin was thoroughly evaluated. Under optimized conditions, 96% of curcumin was converted within 24h into the corresponding curcumin β-glycosides. In addition, the reaction was performed in a coupled system with sucrose synthase from Glycine max, to enable the cost-efficient (re)generation of UDP-Glc from sucrose as abundant and renewable resource.

  13. Supernova Ejecta in the Youngest Galactic Supernova Remnant G1.9+0.3

    CERN Document Server

    Borkowski, K J; Hwang, U; Green, D A; Petre, R; Krishnamurthy, K; Willett, R

    2013-01-01

    G1.9+0.3 is the youngest known Galactic supernova remnant (SNR), with an estimated supernova (SN) explosion date of about 1900, and most likely located near the Galactic Center. Only the outermost ejecta layers with free-expansion velocities larger than about 18,000 km/s have been shocked so far in this dynamically young, likely Type Ia SNR. A long (980 ks) Chandra observation in 2011 allowed spatially-resolved spectroscopy of heavy-element ejecta. We denoised Chandra data with the spatio-spectral method of Krishnamurthy et al., and used a wavelet-based technique to spatially localize thermal emission produced by intermediate-mass elements (IMEs: Si and S) and iron. The spatial distribution of both IMEs and Fe is extremely asymmetric, with the strongest ejecta emission in the northern rim. Fe Kalpha emission is particularly prominent there, and fits with thermal models indicate strongly oversolar Fe abundances. In a localized, outlying region in the northern rim, IMEs are less abundant than Fe, indicating tha...

  14. Site-specific conjugation of bifunctional chelator BAT to mouse IgG1 Fab' fragment

    Institute of Scientific and Technical Information of China (English)

    Jun LI; Xue-hao WANG; Xiao-ming WANG; Zhao-lai CHEN

    2006-01-01

    Aim: To perform a site-specific conjugation of Fab' fragments of a mouse monoclonal antibody(MoAb) B43(of IgG1 subtype) to a bifunctional chelator 6-[p-(bromoacetamido) benzyl]-l,4,8,11-tetraazacyclotetradecane-N,N',N",N'"-tetraacetic acid (BAT) via the thiol groups in the hinge distal to the antigenbinding site of the Fab'. Methods: B43 was cleaved using a simple 2-step method.First, stable F(ab')2 was produced by pepsin treatment. Fab' with free thiol in the hinge region was then obtained by cysteine reduction of F(ab')2. Second, a sitespecific conjugation of Fab' to thiol-specific BAT was performed in a one-step reaction. Results: The Fab' fragment had approximately 1.8 free thiol groups per molecule after cysteine reduction. The conjugation efficiency and the chemical yield were approximately 1.28 moles chelator/Fab' and 74% of the initial concentration of Fab', respectively. The F(ab')2, Fab' and Fab'-BAT all maintained reasonable antigen-binding properties. 67Cu labeling of the conjugate under standard conditions did not impair the immunoreactivity of Fab'-BAT. Conclusion: This is a simple and efficient method for producing immunoreactive conjugates of Fab'-BAT, which can be used to make radiometal-labeled conjugates for further diagnostic and therapeutic applications.

  15. Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1.

    Science.gov (United States)

    Dewitte, Griet; Walmagh, Maarten; Diricks, Margo; Lepak, Alexander; Gutmann, Alexander; Nidetzky, Bernd; Desmet, Tom

    2016-09-10

    UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their potential application in glycosylation processes. Recombinant expression in E. coli provided sufficient amounts of enzyme for the in-depth characterization of the salicylic acid UGT from Capsella rubella (UGT-SACr) and the stevia UGT from Stevia rebaudiana (UGT-76G1Sr). The latter was found to have a remarkably broad specificity with activities on a wide diversity of structures, from aliphatic and branched alcohols, over small phenolics to larger flavonoids, terpenoids and even higher glycoside compounds. As an example for its industrial potential, the glycosylation of curcumin was thoroughly evaluated. Under optimized conditions, 96% of curcumin was converted within 24h into the corresponding curcumin β-glycosides. In addition, the reaction was performed in a coupled system with sucrose synthase from Glycine max, to enable the cost-efficient (re)generation of UDP-Glc from sucrose as abundant and renewable resource. PMID:27378621

  16. Antiviral therapy in chronic hepatitis C (G1 in Russia: cost and effectiveness

    Directory of Open Access Journals (Sweden)

    A. V. Rudakova

    2015-01-01

    Full Text Available Genotype 1 HCV treatment in Russia assume as bitherapy (pegylated interferon – PG plus ribavirin – RBV as three therapy based on HCV protease inhibitor such as telaprevir (TLV, boceprevir (BCV or simeprevir (SMV plus PG/RBV. Medical technologies characterize neither clinical effectiveness, safety profile nor cost-effectiveness so it’s crucial to assess different costs related antiviral regimens. Three therapy costs for naïve patients including TLV, BCV, SMV are higher bitherapy 2,6; 2,5; 3,1 times accordingly. Similar TLV and BCV effectiveness for naïve patients defines TLV or BCV as the preferable 1-st line regimen, depending on regional features of pricing. SMV and TLV efficacy is similar among naïve patients and ralapsers but SMV is affordable for partially responders and non-responders after previous bitherapy. SMV cost is 1,4 times higher vs TLV but SMV has improved tolerability, less drug-drug interactions and shorter course. Insufficient bitherapy effectiveness for G1 HCV (SVR 24 – 39%-55% is required repeated course of three therapy for half of patient population. The first line regimen based on innovation will improve clinical outcomes for more patients and provide cost saving vs previous bitherapy based on PG/RBV. 

  17. Goblet cell carcinoid of the appendix

    Directory of Open Access Journals (Sweden)

    Pahlavan Payam S

    2005-06-01

    Full Text Available Abstract Background Goblet cell carcinoid (GCC of the appendix is a rare neoplasm that share histological features of both adenocarcinoma and carcinoid tumor. While its malignant potential remains unclear, GCC's are more aggressive than conventional carcinoid. The clinical presentations of this neoplasm are also varied. This review summarizes the published literature on GCC of the appendix. The focus is on its diagnosis, histopathological aspects, clinical manifestations, and management. Methods Published studies in the English language between 1966 to 2004 were identified through Medline keyword search utilizing terms "goblet cell carcinoid," "adenocarcinoid", "mucinous carcinoid" and "crypt cell carcinoma" of the appendix. Results Based on the review of 57 published papers encompassing nearly 600 diagnosed patients, the mean age of presentation for GCC of the appendix was 58.89 years with equal representation in both males and females. Accurate diagnosis of this neoplasm requires astute observations within an acutely inflamed appendix as this neoplasm has a prominent pattern of submucosal growth and usually lacks the formation of a well-defined tumor mass. The mesoappendix was involved in 21.64% followed by perineural involvement in 2.06%. The most common clinical presentations in order of frequency were acute appendicitis in 22.5%; asymptomatic in 5.4%; non-localized abdominal pain in 5.15% and an appendicular mass in 3.09%. The most common surgical treatment of choice was appendectomy with right hemicolectomy in 34.70% followed by simple appendectomy in 24.57%. Concomitant distant metastasis at diagnosis was present in 11.16% of patients with the ovaries being the most common site in 3.60% followed by disseminated abdominal carcinomatosis in 1.03%. Local lymph node involvement was seen in 8.76% of patients at the time of diagnosis. The reported 5-year survival ranges from 60 % to 84%. GCC's of the appendix remains a neoplasm of unpredictable

  18. The G-protein-coupled estrogen receptor agonist G-1 suppresses proliferation of ovarian cancer cells by blocking tubulin polymerization

    OpenAIRE

    Wang, C; Lv, X.; C. He; Hua, G; Tsai, M-Y; Davis, J S

    2013-01-01

    The G-protein-coupled estrogen receptor 1 (GPER) has recently been reported to mediate the non-genomic action of estrogen in different types of cells and tissues. G-1 (1-[4-(6-bromobenzo[1,3] dioxol-5yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinolin-8-yl]-ethanone) was developed as a potent and selective agonist for GPER. G-1 has been shown to induce the expression of genes and activate pathways that facilitate cancer cell proliferation by activating GPER. Here we demonstrate that G-1 has an ...

  19. Restricted motion of the conserved immunoglobulin G1 N-glycan is essential for efficient FcγRIIIa binding

    OpenAIRE

    Subedi, Ganesh P.; Hanson, Quinlin M.; Barb, Adam W

    2014-01-01

    Immunoglobulin G1(IgG1)-based therapies are widespread and many function through interactions with low-affinity Fc γ receptors (FcγR). N-glycosylation of the IgG1 Fc domain is required for FcγR binding, though it is unclear why. Structures of the FcγR:Fc complex fail to explain this because the FcγR polypeptide does not bind the N-glycan. Here we identify a link between motion of the N-glycan and Fc:FcγRIIIa affinity that explains the N-glycan requirement. Fc F241 and F243 mutations decreased...

  20. Downregulation of CREB Promotes Cell Proliferation by Mediating G1/S Phase Transition in Hodgkin Lymphoma.

    Science.gov (United States)

    Lu, Fangjin; Zheng, Ying; Donkor, Paul Owusu; Zou, Peng; Mu, Ping

    2016-01-01

    The cyclic-AMP response element-binding protein (CREB), a well-known nuclear transcription factor, has been shown to play an essential role in many cellular processes, including differentiation, cell survival, and cell proliferation, by regulating the expression of downstream genes. Recently, increased expression of CREB was frequently found in various tumors, indicating that CREB is implicated in the process of tumorigenesis. However, the effects of CREB on Hodgkin lymphoma (HL) remain unknown. To clarify the role of CREB in HL, we performed knockdown experiments in HL. We found that downregulation of CREB by short hairpin RNA (shRNA) resulted in enhancement of cell proliferation and promotion of G1/S phase transition, and these effects can be rescued by expression of shRNA-resistant CREB. Meanwhile, the expression level of cell cycle-related proteins, such as cyclin D1, cyclin E1, cyclin-dependent kinase 2 (CDK2), and CDK4, was elevated in response to depletion of CREB. Furthermore, we performed chromatin immunoprecipitation (ChIP) assay and confirmed that CREB directly bound to the promoter regions of these genes, which consequently contributed to the regulation of cell cycle. Consistent with our results, a clinical database showed that high expression of CREB correlates with favorable prognosis in B-cell lymphoma patients, which is totally different from the function of CREB in other cancers such as colorectal cancer, acute myeloid leukemia, and some endocrine cancers. Taken together, all of these features of CREB in HL strongly support its role as a tumor suppressor gene that can decelerate cell proliferation by inhibiting the expression of several cell cycle-related genes. Our results provide new evidence for prognosis prediction of HL and a promising therapeutic strategy for HL patients. PMID:27458098

  1. Induction of G1 cell cycle arrest and apoptosis by berberine in bladder cancer cells.

    Science.gov (United States)

    Yan, Keqiang; Zhang, Cheng; Feng, Jinbo; Hou, Lifang; Yan, Lei; Zhou, Zunlin; Liu, Zhaoxu; Liu, Cheng; Fan, Yidon; Zheng, Baozhong; Xu, Zhonghua

    2011-07-01

    Bladder cancer is the ninth most common type of cancer, and its surgery is always followed by chemotherapy to prevent recurrence. Berberine is non-toxic to normal cells but has anti-cancer effects in many cancer cell lines. This study was aimed to determine whether berberine inhibits the cell proliferation and induces cell cycle arrest and apoptosis in BIU-87 and T24 bladder cancer cell line. The superficial bladder cancer cell line BIU-87 and invasive T24 bladder cancer cells were treated with different concentrations of berberine. MTT assay was used to determine the effects of berberine on the viability of these cells. The cell cycle arrest was detected through propidium iodide (PI) staining. The induction of apoptosis was determined through Annexin V-conjugated Alexa Fluor 488 (Alexa488) staining. Berberine inhibited the viability of BIU-87 and T24 cells in a dose- and time-dependent manner. It also promoted cell cycle arrest at G0/G1 in a dose-dependent manner and induced apoptosis. We observed that H-Ras and c-fos mRNA and protein expressionswere dose-dependently and time-dependently decreased by berberine treatment. Also, we investigated the cleaved caspase-3 and caspase-9 protein expressions increased in a dose-dependent manner. Berberine inhibits the cell proliferation and induces cell cycle arrest and apoptosis in BIU-87, bladder cancer cell line and T24, invasive bladder cancer cell line. Berberine can inhibit the oncogentic H-Ras and c-fos in T24 cells, and can induce the activation of the caspase-3 and caspase-9 apoptosis. Therefore, berberine has the potential to be a novel chemotherapy drug to treat the bladder cancer by suppressing tumor growth.

  2. The impact of geoengineering on vegetation in experiment G1 of the Geoengineering Model Intercomparison Project

    Science.gov (United States)

    Irvine, Peter; Glienke, Susanne; Lawrence, Mark

    2015-04-01

    Solar Radiation Management (SRM) has been proposed as a means to partly counteract global warming. The Geoengineering Model Intercomparison Project (GeoMIP) simulated the climate consequences of a number of SRM techniques, but the effects on vegetation have not yet been thoroughly studied. Here, the vegetation response to the idealized GeoMIP G1 experiment is analyzed, in which a reduction of the solar constant counterbalances the radiative effects of quadrupled atmospheric CO2 concentrations; the results from eight fully coupled earth system models (ESMs) are included. For most models and regions, changes in net primary productivity (NPP) are dominated by the increase in CO2, via the CO2 fertilization effect. As SRM will lower temperatures, in high latitudes this will reverse gains in NPP from the lifting of temperature limitations. In low latitudes this cooling relative to the 4xCO2 simulation decreases plant respiration whilst having little effect on gross primary productivity, increasing NPP. Despite reductions in precipitation in most regions in response to SRM, runoff and NPP increase in many regions including those previously highlighted as potentially being at risk of drought under SRM. This is due to simultaneous reductions in evaporation and increases in water use efficiency by plants due to higher CO2 concentrations. The relative differences between models in the vegetation response are substantially larger than the differences in their climate responses. The largest differences between models are for those with and without a nitrogen-cycle, with a much smaller CO2 fertilization effect for the former. These results suggest that until key vegetation processes are integrated into ESM predictions, the vegetation response to SRM will remain highly uncertain.

  3. Endothelial ATP-binding cassette G1 in mouse endothelium protects against hemodynamic-induced atherosclerosis.

    Science.gov (United States)

    Xue, Shanshan; Wang, Jiaxing; Zhang, Xu; Shi, Ying; Li, Bochuan; Bao, Qiankun; Pang, Wei; Ai, Ding; Zhu, Yi; He, Jinlong

    2016-08-19

    Activated vascular endothelium inflammation under persistent hyperlipidemia is the initial step of atherogenesis. ATP-binding cassette G1 (ABCG1) is a crucial factor maintaining sterol and lipid homeostasis by transporting cholesterol efflux to high-density lipoprotein. In this study, we investigated the protective effects of ABCG1 in endothelial inflammation activation during early-stage atherogenesis in mice and the underlying mechanisms. Endothelial cell (EC)-specific ABCG1 transgenic (EC-ABCG1-Tg) mice were generated and cross-bred with low-density lipoprotein receptor-deficient (Ldlr(-/-)) mice. After a 4-week Western-type diet, the mice were sacrificed for assessing atherosclerosis. Human umbilical vein ECs were treated with different flows, and ABCG1 was adenovirally overexpressed to investigate the mechanism in vitro. Compared with Ldlr(-/-) mouse aortas, EC-ABCG1-Tg/Ldlr(-/-) aortas showed decreased early-stage lesions. Furthermore, the lesion area in the EC-ABCG1-Tg/Ldlr(-/-) mouse aortic arch but not thoracic aorta was significantly reduced, which suggests a protective role of ABCG1 under atheroprone flow. In vitro, overexpression of ABCG1 attenuated EC activation caused by oscillatory shear stress. Overexpression of ABCG1 blunted cholesterol-activated ECs in vitro. In exploring the mechanisms of ABCG1 attenuating endothelial inflammation, we found that ABCG1 inhibited oscillatory flow-activated nuclear factor kappa B and NLRP3 inflammasome in ECs. ABCG1 may play a protective role in early-stage atherosclerosis by reducing endothelial activation induced by oscillatory shear stress via suppressing the inflammatory response. PMID:27297110

  4. Biosensor-based screening method for the detection of aflatoxins B1-G1.

    Science.gov (United States)

    Cuccioloni, Massimiliano; Mozzicafreddo, Matteo; Barocci, Simone; Ciuti, Francesca; Pecorelli, Ivan; Eleuteri, Anna Maria; Spina, Michele; Fioretti, Evandro; Angeletti, Mauro

    2008-12-01

    Aflatoxins are extremely toxic metabolites from Aspergillus species that can adulterate a wide range of human foodstuff. Herein, we propose a novel assay designed as an analytical test for aflatoxin B1 and G1 (AFB1 and AFG1, respectively) that could represent an alternative screening technique for this class of mycotoxins. The approach for the determination of these toxins is based on surface plasmon resonance using neutrophil porcine elastase as a "bait" for these aflatoxins. The selection and optimization of the analytical procedure involved a preliminary investigation on the type of inhibition by AFB1: the level of the protease inhibition exerted by AFB1 depended upon the incubation time and the concentration of the binding partners, showing the competitiveness and the reversibility of the inhibition. A posteriori, the nature of the interaction granted a rapid analysis, a single detection test requiring only a few minutes. For the development of the assay, the experimental conditions were evaluated and optimized with both calibration solution and aflatoxin-spiked samples. To apply this method to aflatoxin-contaminated maize, a rapid solid-phase extraction treatment was developed. The proposed assay for AFB1 and AFG1 was validated by comparison with both a chromatographic reference method and a standard enzyme linked immunosorbent assay procedure. This enzyme-based biosensor represents a new approach for the detection of aflatoxins based on the reversible interaction between a blocked macromolecule and a soluble ligand, having the major advantages in the relative rapidity, the reusability of the capturing surface, and low cost per single test. PMID:19551989

  5. Increased expression of bcl11b leads to chemoresistance accompanied by G1 accumulation.

    Directory of Open Access Journals (Sweden)

    Piotr Grabarczyk

    Full Text Available BACKGROUND: The expression of BCL11B was reported in T-cells, neurons and keratinocytes. Aberrations of BCL11B locus leading to abnormal gene transcription were identified in human hematological disorders and corresponding animal models. Recently, the elevated levels of Bcl11b protein have been described in a subset of squameous cell carcinoma cases. Despite the rapidly accumulating knowledge concerning Bcl11b biology, the contribution of this protein to normal or transformed cell homeostasis remains open. METHODOLOGY/PRINCIPAL FINDINGS: Here, by employing an overexpression strategy we revealed formerly unidentified features of Bcl11b. Two different T-cell lines were forced to express BCL11B at levels similar to those observed in primary T-cell leukemias. This resulted in markedly increased resistance to radiomimetic drugs while no influence on death-receptor apoptotic pathway was observed. Apoptosis resistance triggered by BCL11B overexpression was accompanied by a cell cycle delay caused by accumulation of cells at G1. This cell cycle restriction was associated with upregulation of CDKN1C (p57 and CDKN2C (p18 cyclin dependent kinase inhibitors. Moreover, p27 and p130 proteins accumulated and the SKP2 gene encoding a protein of the ubiquitin-binding complex responsible for their degradation was repressed. Furthermore, the expression of the MYCN oncogene was silenced which resulted in significant depletion of the protein in cells expressing high BCL11B levels. Both cell cycle restriction and resistance to DNA-damage-induced apoptosis coincided and required the histone deacetylase binding N-terminal domain of Bcl11b. The sensitivity to genotoxic stress could be restored by the histone deacetylase inhibitor trichostatine A. CONCLUSIONS: The data presented here suggest a potential role of BCL11B in tumor survival and encourage developing Bcl11b-inhibitory approaches as a potential tool to specifically target chemoresistant tumor cells.

  6. Nicotine overrides DNA damage-induced G1/S restriction in lung cells.

    Directory of Open Access Journals (Sweden)

    Takashi Nishioka

    Full Text Available As an addictive substance, nicotine has been suggested to facilitate pro-survival activities (such as anchorage-independent growth or angiogenesis and the establishment of drug resistance to anticancer therapy. Tobacco smoking consists of a variety of carcinogens [such as benzopyrene (BP and nitrosamine derivatives] that are able to cause DNA double strand breaks. However, the effect of nicotine on DNA damage-induced checkpoint response induced by genotoxins remains unknown. In this study, we investigated the events occurred during G(1 arrest induced by γ-radiation or BP in nicotine-treated murine or human lung epithelial cells. DNA synthesis was rapidly inhibited after exposure to γ-radiation or BP treatment, accompanied with the activation of DNA damage checkpoint. When these cells were co-treated with nicotine, the growth restriction was compromised, manifested by upregulation of cyclin D and A, and attenuation of Chk2 phosphorylation. Knockdown of cyclin D or Chk2 by the siRNAs blocked nicotine-mediated effect on DNA damage checkpoint activation. However, nicotine treatment appeared to play no role in nocodazole-induced mitotic checkpoint activation. Overall, our study presented a novel observation, in which nicotine is able to override DNA damage checkpoint activated by tobacco-related carcinogen BP or γ-irradiation. The results not only indicates the potentially important role of nicotine in facilitating the establishment of genetic instability to promote lung tumorigenesis, but also warrants a dismal prognosis for cancer patients who are smokers, heavily exposed second-hand smokers or nicotine users.

  7. Republic of Congo; Selected Issues and Statistical Appendix

    OpenAIRE

    International Monetary Fund

    2004-01-01

    This Selected Issues and Statistical Appendix paper outlines the recent developments in the political and security situation in Congo. It reviews economic performance during 1970–2003, including in the context of IMF-supported programs. The paper also reviews recent developments in public finance management, and examines the constraints on growth and poverty reduction. The sources of economic growth during 1970–2003 are analyzed. The paper also discusses the feasibility of an oil fiscal r...

  8. Activity in mice of recombinant BCG-EgG1Y162 vaccine for Echinococcus granulosus infection.

    Science.gov (United States)

    Ma, Xiumin; Zhao, Hui; Zhang, Fengbo; Zhu, Yuejie; Peng, Shanshan; Ma, Haimei; Cao, Chunbao; Xin, Yan; Yimiti, Delixiati; Wen, Hao; Ding, Jianbing

    2016-01-01

    Cystic hydatid disease is a zoonotic parasitic disease caused by Echinococcus granulosus which is distributed worldwide. The disease is difficult to treat with surgery removal is the only cure treatment. In the high endemic areas, vaccination of humans is believed a way to protect communities from the disease. In this study we vaccinated BALB/c mice with rBCG-EgG1Y162, and then detected the level of IgG and IgE specifically against the recombinant protein by ELISA, rBCG-EgG1Y162 induced strong and specific cellular and humoral immune responses. In vitro study showed that rBCG-EgG1Y162 vaccine not only promote splenocytes proliferation but also active T cell. In addition, the rBCG-EgG1Y162 induced a protection in the mice against secondary infection of Echinococcus granulosus.

  9. Appendix: Marketing and Student Recruitment Practices for Master's-Level Graduate Programs, 2012. Trends in Enrollment Management

    Science.gov (United States)

    Noel-Levitz, Inc, 2012

    2012-01-01

    This paper presents the appendix to "Marketing and Student Recruitment Practices for Master's-Level Graduate Programs, 2012" report. Included in this appendix are: (1) Ratings of the primary practices measured in this study, displayed by institution type; (2) The practice of purchasing prospective student names and addresses; (3) Ratings of…

  10. 12 CFR Appendix B to Part 748 - Guidance on Response Programs for Unauthorized Access to Member Information and Member Notice

    Science.gov (United States)

    2010-01-01

    ... See 12 CFR Part 748, appendix A, Paragraph III.B. 2. Following the assessment of these risks, appendix... Safeguards Rule promulgated by the Federal Trade Commission (“FTC”), 12 CFR Part 314. II. Response Program i... containing nonpublic personal information about a member, whether in paper, electronic, or other...

  11. Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator

    OpenAIRE

    Steffensen, Knut R; Bouzga, Mariam; Skjeldal, Frode; Kasi, Cecilie; Karahasan, Almira; Matre, Vilborg; Bakke, Oddmund; Guérin, Sylvain; Eskild, Winnie

    2007-01-01

    Background Novel, uncharacterised proteins represent a challenge in biochemistry and molecular biology. In this report we present an initial functional characterization of human kidney predominant protein, NCU-G1. Results NCU-G1 was found to be a highly conserved nuclear protein rich in proline with a molecular weight of approximately 44 kDa. It is localized on chromosome 1 and consists of 6 exons. An...

  12. Characterization of a G1 inhibitor from old JB-1 ascites tumor fluid. Interaction with polyions and ion exchangers.

    Science.gov (United States)

    Barfod, N M; Bichel, P

    1976-09-17

    In most experimental ascites tumors the growth rate decreases with increasing age and cell number. This decrease is caused by a prolongation of the cell cycle and an increasing accumulation of noncycling cells in resting (or quiescent) G1 and G2 compartments. In cell-free ascitic fluid from the JB-1 ascites tumor in the plateau phase of growth, low molecular weight substances have been found which reversibly and specifically arrest JB-1 cells in G1 and G2. In order to characterize the JB-1 G1 inhibitor we have investigated the effect of ion exchangers and polyions on the activity of this inhibitor assayed in vitro by means of a partially synchronized JB-1 cell population analyzed by flow microfluorometry. The results indicate that polyanions and cation exchangers (immobilized polyanions) bind and abolish the G1-inhibitory activity. From this it is suggested that the G1 inhibitor is of a basic or polycationic nature. Since anion exchangers (immobilized polycations) are without effect on this activity it was surprising to find that polycations also neutralize the activity. The results indicate that this occurs by blocking an anionic G2-inhibitor receptor on the cell, thus preventing the polycationic G1 inhibitor from being bound to this receptor.

  13. Whi5 phosphorylation embedded in the G1/S network dynamically controls critical cell size and cell fate.

    Science.gov (United States)

    Palumbo, Pasquale; Vanoni, Marco; Cusimano, Valerio; Busti, Stefano; Marano, Francesca; Manes, Costanzo; Alberghina, Lilia

    2016-01-01

    In budding yeast, overcoming of a critical size to enter S phase and the mitosis/mating switch--two central cell fate events--take place in the G1 phase of the cell cycle. Here we present a mathematical model of the basic molecular mechanism controlling the G1/S transition, whose major regulatory feature is multisite phosphorylation of nuclear Whi5. Cln3-Cdk1, whose nuclear amount is proportional to cell size, and then Cln1,2-Cdk1, randomly phosphorylate both decoy and functional Whi5 sites. Full phosphorylation of functional sites releases Whi5 inhibitory activity, activating G1/S transcription. Simulation analysis shows that this mechanism ensures coherent release of Whi5 inhibitory action and accounts for many experimentally observed properties of mitotically growing or conjugating G1 cells. Cell cycle progression and transcriptional analyses of a Whi5 phosphomimetic mutant verify the model prediction that coherent transcription of the G1/S regulon and ensuing G1/S transition requires full phosphorylation of Whi5 functional sites. PMID:27094800

  14. FACT prevents the accumulation of free histones evicted from transcribed chromatin and a subsequent cell cycle delay in G1.

    Directory of Open Access Journals (Sweden)

    Macarena Morillo-Huesca

    2010-05-01

    Full Text Available The FACT complex participates in chromatin assembly and disassembly during transcription elongation. The yeast mutants affected in the SPT16 gene, which encodes one of the FACT subunits, alter the expression of G1 cyclins and exhibit defects in the G1/S transition. Here we show that the dysfunction of chromatin reassembly factors, like FACT or Spt6, down-regulates the expression of the gene encoding the cyclin that modulates the G1 length (CLN3 in START by specifically triggering the repression of its promoter. The G1 delay undergone by spt16 mutants is not mediated by the DNA-damage checkpoint, although the mutation of RAD53, which is otherwise involved in histone degradation, enhances the cell-cycle defects of spt16-197. We reveal how FACT dysfunction triggers an accumulation of free histones evicted from transcribed chromatin. This accumulation is enhanced in a rad53 background and leads to a delay in G1. Consistently, we show that the overexpression of histones in wild-type cells down-regulates CLN3 in START and causes a delay in G1. Our work shows that chromatin reassembly factors are essential players in controlling the free histones potentially released from transcribed chromatin and describes a new cell cycle phenomenon that allows cells to respond to excess histones before starting DNA replication.

  15. Canonical Wnt activity regulates trunk neural crest delamination linking BMP/noggin signaling with G1/S transition.

    Science.gov (United States)

    Burstyn-Cohen, Tal; Stanleigh, Jonathan; Sela-Donenfeld, Dalit; Kalcheim, Chaya

    2004-11-01

    Delamination of premigratory neural crest cells depends on a balance between BMP/noggin and on successful G1/S transition. Here, we report that BMP regulates G1/S transition and consequent crest delamination through canonical Wnt signaling. Noggin overexpression inhibits G1/S transition and blocking G1/S abrogates BMP-induced delamination; moreover, transcription of Wnt1 is stimulated by BMP and by the developing somites, which concomitantly inhibit noggin production. Interfering with beta-catenin and LEF/TCF inhibits G1/S transition, neural crest delamination and transcription of various BMP-dependent genes, which include Cad6B, Pax3 and Msx1, but not that of Slug, Sox9 or FoxD3. Hence, we propose that developing somites inhibit noggin transcription in the dorsal tube, resulting in activation of BMP and consequent Wnt1 production. Canonical Wnt signaling in turn stimulates G1/S transition and generation of neural crest cell motility independently of its proposed role in earlier neural crest specification. PMID:15456730

  16. Binding of fusion protein FLSC IgG1 to CCR5 is enhanced by CCR5 antagonist Maraviroc.

    Science.gov (United States)

    Latinovic, Olga; Schneider, Kate; Szmacinski, Henryk; Lakowicz, Joseph R; Heredia, Alonso; Redfield, Robert R

    2014-12-01

    The CCR5 chemokine receptor is crucial for human immunodeficiency virus type 1 (HIV-1) infection, acting as the principal coreceptor for HIV-1 entry and transmission and is thus an attractive target for antiviral therapy. Studies have suggested that CCR5 surface density and its conformational changes subsequent to virion engagement are rate limiting for entry, and consequently, infection. Not all CCR5 antibodies inhibit HIV-1 infection, suggesting a need for more potent reagents. Here we evaluated full length single chain (FLSC) IgG1, a novel IgG-CD4-gp120(BAL) fusion protein with several characteristics that make it an attractive candidate for treatment of HIV-1 infections, including bivalency and a potentially increased serum half-life over FLSC, the parental molecule. FLSC IgG1 binds two domains on CCR5, the N-terminus and the second extracellular loop, lowering the levels of available CCR5 viral attachment sites. Furthermore, FLSC IgG1 synergizes with Maraviroc (MVC), the only licensed CCR5 antagonist. In this study, we used both microscopy and functional assays to address the mechanistic aspects of the interactions of FLSC IgG1 and MVC in the context of CCR5 conformational changes and viral infection. We used a novel stochastic optical reconstruction microscopy (STORM), based on high resolution localization of photoswitchable dyes to visualize direct contacts between FLSC IgG1 and CCR5. We compared viral entry inhibition by FLSC IgG1 with that of other CCR5 blockers and showed FLSC IgG1 to be the most potent. We also showed that lower CCR5 surface densities in HIV-1 infected primary cells result in lower FLSC IgG1 EC50 values. In addition, CCR5 binding by FLSC IgG1, but not CCR5 Ab 2D7, was significantly increased when cells were treated with MVC, suggesting MVC allosterically increases exposure of the FLSC IgG1 binding site. These data have implications for future antiviral therapy development.

  17. The Queue Mξ/G/1 with Different Arrival Rates on the Multiple Adaptive Vacations and Setup Times%具有不同到达率的带有启动时间的多级适应性休假Mξ/G/1排队模型

    Institute of Scientific and Technical Information of China (English)

    孙微; 李世勇; 田乃硕

    2007-01-01

    In this paper, we discuss the queue Mξ/G/1 with different arrival rates on the multiple adaptive vacations and setup times. We derive the probability generating function (p.g.f.)of the steady-state queue length by the method of embedded Markov chain and the Laplace-Stieltjes transform (LST)of the steady-state waiting time (FCFS). From the results, we obtain the conclusion that the steady-state queue length and waiting time have the property of stochastic decomposition. And we also get the LST of busy period and give several special cases. Many discussed models as to Mξ/G/1 are special cases of the model.%本文研究具有不同到达率的带有启动时间的多级适应性休假Mξ/G/1排队模型,应用嵌入马尔可夫链方法推导出了稳态队长和等待时间(先到先服务规则)分布,并验证了稳态队长和稳态等待时间具有随机分解性,而且给出了忙期分布.许多关于Mξ/G/1的排队模型都可以看作是此模型的特例.

  18. Staurosporine is chemoprotective by inducing G1 arrest in a Chk1- and pRb-dependent manner.

    Science.gov (United States)

    Murray, Mollianne McGahren; Bui, Tuyen; Smith, Michelle; Bagheri-Yarmand, Rozita; Wingate, Hannah; Hunt, Kelly K; Keyomarsi, Khandan

    2013-10-01

    Chemotherapeutic agents have been the mainstay of cancer therapy for years. However, their effectiveness has been limited by toxicities they impart on normal cells. Staurosporine (ST) has been shown to arrest normal, but not breast cancer, cells in G1. Therefore, ST may become a chemoprotective agent, arresting normal cells while allowing tumor cells to enter cell cycle phases where they are sensitive to chemotherapeutic agents. Understanding the mechanism of ST-mediated G1 arrest may allow for a beneficial chemoprotective treatment strategy for patients. We utilized 76NE6 (pRb+/p53-), 76NF2V (pRb+/p53+) and 76NE7 (pRb-/P53+) non-tumorigenic human mammary epithelial cell lines to understand the role of the Rb and p53 pathways in ST-directed G1 arrest. CDK4 was downregulated by ST in Rb+ cells, but its presence could not reverse the arrest, neither did its stable downregulation alter ST-mediated cellular response. ST-mediated G1 arrest required pRb, which in turn initiated a cascade of events leading to inhibition of CDK4. Further assessment of this pathway revealed that Chk1 expression and activity were required for the Rb-dependent arrest. For example, pRb+ cells with small interfering RNA to Chk1 had approximately 60% less cells in G1 phase compared with controls and pRb- cells do not arrest upon ST. Furthermore, Chk1 expression facilitates the release of the Rb+ cells from G1 arrest. Collectively, our data suggest that pRb cooperates with Chk1 to mediate a G1 arrest only in pRb+ cells. The elucidation of this pathway can help identify novel agents to protect cancer patients against the debilitating effects of chemotherapy.

  19. Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator

    Directory of Open Access Journals (Sweden)

    Bakke Oddmund

    2007-11-01

    Full Text Available Abstract Background Novel, uncharacterised proteins represent a challenge in biochemistry and molecular biology. In this report we present an initial functional characterization of human kidney predominant protein, NCU-G1. Results NCU-G1 was found to be a highly conserved nuclear protein rich in proline with a molecular weight of approximately 44 kDa. It is localized on chromosome 1 and consists of 6 exons. Analysis of the amino acid sequence revealed no known transcription activation domains or DNA binding regions, however, four nuclear receptor boxes (LXXLL, and four SH3-interaction motives in addition to numerous potential phosphorylation sites were found. Two nuclear export signals were identified, but no nuclear localization signal. In man, NCU-G1 was found to be widely expressed at the mRNA level with especially high levels detected in prostate, liver and kidney. Electrophoretic mobility shift analysis showed specific binding of NCU-G1 to an oligonucleotide representing the footprint 1 element of the human cellular retinol-binding protein 1 gene promoter. NCU-G1 was found to activate transcription from this promoter and required presence of the footprint 1 element. In transiently transfected Drosophila Schneider S2 cells, we demonstrated that NCU-G1 functions as a co-activator for ligand-activated PPAR-alpha, resulting in an increased expression of a CAT reporter gene under control of the peroxisome proliferator-activated receptor-alpha responsive acyl-CoA oxidase promoter. Conclusion We propose that NCU-G1 is a dual-function protein capable of functioning as a transcription factor as well as a nuclear receptor co-activator.

  20. Isolation and characteristics of autoreactive T cells spe cific to aggrecan G1 domain from rheumatoid arthritis patients

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Our previous work showed that the cartilage proteo glycan aggrecan could induce an erosive polyarthritis and spondylitis in BALB/c mice and the G1 globular domain of the aggrecan (G1) contained the arthritogenic region. To elucidate whether autoreactive T cells to G1 are ex pressed in rheumatoid arthritis patients, we analyzed the frequency of human G1-specific T cells in the peripheral blood of five rheumatoid arthritis patients and tried to establish G1-reactive T cell lines from these rheumatoid arthritis patients. The results showed that the Gl-specific T cells in PBL were detectable at the range of 4.97 + 0.5 × 10-6 in peripheral blood lymphocytes. We have also generated 15 G1-specific T lymphocyte lines from these pateints with a standard split-well method. All these cells expressed fine specificity to human recombinant G1, but not to unrelated antigen. All the 15 lines expressed a pan T cell marker and 13 of them selectively used the αβ T cell receptor. Two of them used γδ T cell receptor. The 13 of these T cell lines was CD4 positive. One line expressed CD8. One line expressed both CD4 and CD8. More over, 14 out of 15 lines expressed the Th-1 cytokine profile, characterized by interferon-γ positivity and IL-4 negativ ity. No Th-2 type cell line was generated. These data provide strong evidence in favor of the presence of autore active T cells in the rheumatoid arthritis pateints. What is the mechanism(s) that these autoreactive T cells attack self-target and whether these Gl-specific, Th-1 type T cell lines can induce arthritis in immune deficiency mice are currently under investigation.

  1. Determination of aflatoxins B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil.

    Science.gov (United States)

    Bao, Lei; Trucksess, Mary W; White, Kevin D

    2010-01-01

    Edible oils are consumed directly, and used as ingredients in food, soaps, and skin products. However, oils such as olive oil, peanut oil, and sesame oil could be contaminated with aflatoxins, which are detrimental to human and animal health. A method using immunoaffinity column cleanup with RPLC separation and fluorescence detection (FLD) for determination of aflatoxins (AF) B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil was developed and validated. Test samples were extracted with methanol-water (55 + 45, v/v). After shaking and centrifuging, the lower layer was filtered, diluted with water, and filtered through glass microfiber filter paper. The filtrate was then passed through an immunoaffinity column, and the toxins were eluted with methanol. The toxins were then subjected to RPLC/FLD analysis after postcolumn UV photochemical derivatization. The accuracy and repeatability characteristics of the method were determined. Recoveries of AFB1 spiked at levels from 1.0 to 10.0 microg/kg in olive oil, peanut oil, and sesame oil ranged from 82.9 to 98.6%. RSDs ranged from 0.6 to 8.9%. HorRat values were < 0.2 for all of the matrixes tested. Recoveries of AF spiked at levels from 2.0 to 20.0 microg/kg ranged from 87.7 to 102.2%. RSDs ranged from 1.3 to 12.6%. HorRat values were < 0.4 for all of the matrixes tested. LC/MS/MS with multiple-reaction monitoring was used to confirm the identities of aflatoxins in a naturally contaminated peanut oil. PMID:20629398

  2. Impacts, Effectiveness and Regional Inequalities of the GeoMIP G1 to G4 Solar Radiation Management Scenarios

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Xiaoyong; Moore, John; Cui, Xuefeng; Rinke, Annette; Ji, Duoying; Kravitz, Benjamin S.; Yoon, Jin-Ho

    2015-06-01

    We evaluate the regional effectiveness of solar radiation management (SRM) to compensate for simultaneous changes in temperature and precipitation induced by increased greenhouse gas concentrations. We analyze results from multiple earth system models under four Geoengineering Model Intercomparison Project(GeoMIP) experiments with a modified form of the Residual Climate Response approach. Under the solar dimming geoengineering experiments G1(4xCO2) and G2(increasing CO2 by 1% per year), global average temperature is successfully restored to pre-industrial level over 50 years simulations. However, these two SRM experiments also produce a robust global precipitation decrease. The stratospheric aerosol GeoMIP geoengineering experiment, G4 has significantly greater regional inequality and lower effectiveness for compensating temperature change than G1 and G2. G4 also has significantly larger regional inequality for compensating precipitation change than G1and G2. However, there is no significant difference between precipitation change compensation effectiveness of G4 and G2, though there is much larger across model variability in G4 results. G3 has significant greater regional inequality for compensating temperature change than G1 and G2, and has significant lower effectiveness than G1. The effectiveness of four SRMs to compensate for temperature change is much higher than for precipitation. The large cross-model variation in adjustment percentage of compensated SAT and precipitation change by SRM to achieve optimal compensation effectiveness shed a light on the uncertainty accumulation effect in optimizing compensation effectiveness of SRM.

  3. Precise determination of the spin structure function $\\mathbf{g_1}$ of the proton, deuteron and neutron

    CERN Document Server

    Airapetian, A; Akopov, Z; Andrus, A; Aschenauer, E C; Augustyniak, W; Avakian, R; Avetisian, A; Avetissian, E; Belostotskii, S; Bianchi, N; Blok, H P; Böttcher, H; Borisov, A; Borysenko, A; Brüll, A; Bryzgalov, V; Capiluppi, M; Capitani, G P; Ciullo, G; Contalbrigo, M; Dalpiaz, P F; Deconinck, W; De Leo, R; Demey, M; De Nardo, L; De Sanctis, E; Devitsin, E; Diefenthaler, M; Di Nezza, P; Dreschler, J; Düren, M; Ehrenfried, M; Elalaoui-Moulay, A; Elbakian, G; Ellinghaus, F; Elschenbroich, U; Fabbri, R; Fantoni, A; Felawka, L; Frullani, S; Funel, A; Gabbert, D; Gärber, Y; Gapienko, G; Gapienko, V; Garibaldi, F; Garrow, K; Gavrilov, G; Karibian, V; Giordano, F; Grebenyuk, O; Gregor, I M; Guler, H; Gute, A; Hadjidakis, C; Hartig, M; Hasch, D; Hasegawa, T; Hesselink, W H A; Hillenbrand, A; Hoek, M; Holler, Y; Hommez, B; Hristova, I; Iarygin, G; Ivanilov, A; Izotov, A; Jackson, H E; Jgoun, A; Kaiser, R; Keri, T; Kinney, E; Kiselev, A; Kobayashi, T; Kopytin, M; Korotkov, V; Kozlov, V; Krauss, B; Kravchenko, P; Krivokhizhin, V G; Lagamba, L; Lapikas, L; Lenisa, P; Liebing, P; Linden-Levy, L A; Lorenzon, W; Lü, J; Lu, S; Ma, B Q; Maiheu, B; Makins, N C R; Mao, Y; Marianski, B; Marukyan, H; Masoli, F; Mexner, V; Meyners, N; Michler, T; Miklukho, O; Miller, C A; Miyachi, Y; Muccifora, V; Murray, M; Nagaitsev, A; Nappi, E; Naryshkin, Yu; Negodaev, M; Nowak, Wolf-Dieter; Ohsuga, H; Osborne, A; Perez-Benito, R; Pickert, N; Raithel, M; Reggiani, D; Reimer, P E; Reischl, A; Reolon, A R; Riedl, C; Rith, K; Rosner, G; Rostomyan, A; Rubacek, L; Rubin, J; Ryckbosch, D; Salomatin, Y; Sanjiev, I; Savin, I; Schäfer, A; Schnell, G; Schüler, K P; Seele, J; Seitz, B; Shearer, C; Shibata, T A; Shutov, V; Sinram, K; Stancari, M; Statera, M; Steffens, E; Steijger, J J M; Stenzel, H; Stewart, J; Stinzing, F; Stösslein, U; Streit, J; Tait, P; Tanaka, H; Taroian, S P; Tchuiko, B; Terkulov, A R; Trzcinski, A; Tytgat, M; Vandenbroucke, A; Van der Nat, P B; van der Steenhoven, G; Van Haarlem, Y; Veretennikov, D; Vikhrov, V; Vogel, C; Wang, S; Weiskopf, C; Ye, Y; Ye, Z; Yen, S; Zihlmann, B; Zupranski, P

    2006-01-01

    Precise measurements of the spin structure functions of the proton $g_1^p(x,Q^2)$ and deuteron $g_1^d(x,Q^2)$ are presented over the kinematic range $0.0041 \\leq x \\leq 0.9$ and $0.18 $ GeV$^2$ $\\leq Q^2 \\leq 20$ GeV$^2$. The data were collected at the HERMES experiment at DESY, in deep-inelastic scattering of 27.6 GeV longitudinally polarized positrons off longitudinally polarized hydrogen and deuterium gas targets internal to the HERA storage ring. The neutron spin structure function $g_1^n$ is extracted by combining proton and deuteron data. The integrals of $g_1^{p,d}$ at $Q^2=5$ GeV$^2$ are evaluated over the measured $x$ range. Neglecting any possible contribution to the $g_1^d$ integral from the region $x \\leq 0.021$, a value of $0.330 \\pm 0.011\\mathrm{(theo.)}\\pm0.025\\mathrm{(exp.)}\\pm 0.028$(evol.) is obtained for the flavor-singlet axial charge $a_0$ in a leading-twist NNLO analysis.

  4. Cloning, sequence analysis, and expression of the genes encoding lytic functions of Bacteriophage phi g1e.

    Science.gov (United States)

    Oki, M; Kakikawa, M; Yamada, K; Taketo, A; Kodaira, K I

    1996-10-17

    The lysis genes of a Lactobacillus phage phi g1e were cloned, sequenced, and expressed in Escherichia coli. Nucleotide sequencing of a 3813-bp phi g1e DNA revealed five successive open reading frames (ORF), Rorf50, Rorf118, hol, and lys and Rorf175, in the same DNA strand. By comparative analysis of the DNA sequence, the putative hol product (holin) has an estimated molecular weight is 14.2 kDa, and contains two potential transmembrane helices and highly charged N- and C-termini, resembling predicted holins (which are thought to be a cytoplasmic membrane-disrupting protein) encoded by other phages such as mv1 from Lactobacillus bulgaricus, phi adh from Lactobacillus gasseri, as well as monocins from Listeria. On the other hand, the putative phi g1e lys product (lysin) of 48.4 kDa shows significant similarity with presumed muramidase, known as a cell wall peptidoglycandegrading enzyme, encoded by the Lactobacillus phage mv1 and phi adh, the Lactococcus lactis phage phi LC3, and the Streptococcus pneumoniae phages Cp-1, Cp-7 and Cp-9. When expressed in E. coli, the phi g1e lysin and/or holin decreased the cell turbidity significantly, suggesting that the phi g1e hol-lys system is involved in cytolytic process. PMID:8918256

  5. Stable Expression of Hantavirus H8205 Strain G1/IL-2 Gene and Immune Protection of the Fusion Gene

    Institute of Scientific and Technical Information of China (English)

    XIONG Ying; YUAN Yuan; JIA Min; YU Bing; HUANG Hanju

    2007-01-01

    To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant eukaryotic expression vector containing Hantavirus G1 gene and IL-2 gene. With the help of lipofectamine, the Vero cells were transfected with pcDNA3.1/HisB-IL-2-G1 and the positive cells were selected by G418. IFAT and SDS-PAGE electrophoresis were used to determine the stable transfection and expression of recombinant protein.Each mouse was inoculated with plasmids intramuscularly (i.m.) three times, 2 boosts were given at 2-week intervals, serum anti-hantavirus antibodies were detected by ELISA and neutralizing antibodies (NAb) were detected by Plaque Reduction Neutralization Test. The fusion protein expressed in Vero cells was 78 kD, corresponding to the estimated molecular size. The neutralizing antibody titers of mice with pcDNA3.1/HisB-IL-2-G1 were 1:20-1:80. IL-2/G1 fusion gene could be transferred in Vero cells and stably express the fusion protein. Specific humeral immune responses in mice can be induced with the recombinant eukaryotic expression vector containing the fusion gene, which lays the foundation for further development of therapeutic HTNV vaccine.

  6. Expression and Antigenic Characterization of the Epitope-G1 of the Bovine Ephemeral Fever Virus Glycoprotein in Pichia pastoris

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The epitope-G1 gene of Bovine ephemeral fever virus (BEFV) glycoprotein was synthesised by PCR and cloned into expression vector pPIC9K to construct recombinant plasmid pPIC9K-G1. Then the pPIC9K-G1 was linearized and transformed into Pichia pastoris GS 115. The recombinant P. pastoris strains were selected by a G418 transformation screen and confirmed by PCR. After being induced with methanol, an expressed protein with 26 kDa molecular weight was obtained, which was much bigger than the predicted size (15.54 kDa). Deglycosylation analysis indicated the recombinant G1 was glycosylated. Western blot and ELISA tests, as well as rabbit immunization and specificity experiments indicated that the target protein had both higher reaction activity and higher immunocompetence and specificity. The recombinant G1 protein could be used as a coating antigen to develop an ELISA kit for bovine ephemeral fever diagnosis.

  7. The spin structure function g1p of the proton and a test of the Bjorken sum rule

    Directory of Open Access Journals (Sweden)

    C. Adolph

    2016-02-01

    Full Text Available New results for the double spin asymmetry A1p and the proton longitudinal spin structure function g1p are presented. They were obtained by the COMPASS Collaboration using polarised 200 GeV muons scattered off a longitudinally polarised NH3 target. The data were collected in 2011 and complement those recorded in 2007 at 160 GeV, in particular at lower values of x. They improve the statistical precision of g1p(x by about a factor of two in the region x≲0.02. A next-to-leading order QCD fit to the g1 world data is performed. It leads to a new determination of the quark spin contribution to the nucleon spin, ΔΣ, ranging from 0.26 to 0.36, and to a re-evaluation of the first moment of g1p. The uncertainty of ΔΣ is mostly due to the large uncertainty in the present determinations of the gluon helicity distribution. A new evaluation of the Bjorken sum rule based on the COMPASS results for the non-singlet structure function g1NS(x,Q2 yields as ratio of the axial and vector coupling constants |gA/gV|=1.22±0.05 (stat.±0.10 (syst., which validates the sum rule to an accuracy of about 9%.

  8. The Spin Structure Function $g_1^{\\rm p}$ of the Proton and a Test of the Bjorken Sum Rule

    CERN Document Server

    Adolph, C.; Alexeev, M.G.; Alexeev, G.D.; Amoroso, A.; Andrieux, V.; Anosov, V.; Austregesilo, A.; Azevedo, C.; Badelek, B.; Balestra, F.; Barth, J.; Baum, G.; Beck, R.; Bedfer, Y.; Bernhard, J.; Bicker, K.; Bielert, E.R.; Birsa, R.; Bisplinghoff, J.; Bodlak, M.; Boer, M.; Bordalo, P.; Bradamante, F.; Braun, C.; Bressan, A.; Buchele, M.; Burtin, E.; Capozza, L.; Chang, W.C.; Chiosso, M.; Choi, I.; Chung, S.U.; Cicuttin, A.; Crespo, M.L.; Curiel, Q.; Dalla Torre, S.; Dasgupta, S.S.; Dasgupta, S.; Denisov, O.Yu.; Dhara, L.; Donskov, S.V.; Doshita, N.; Duic, V.; Dziewiecki, M.; Efremov, A.; Eversheim, P.D.; Eyrich, W.; Ferrero, A.; Finger, M.; M. Finger jr; Fischer, H.; Franco, C.; von Hohenesche, N. du Fresne; Friedrich, J.M.; Frolov, V.; Fuchey, E.; Gautheron, F.; Gavrichtchouk, O.P.; Gerassimov, S.; Giordano, F.; Gnesi, I.; Gorzellik, M.; Grabmuller, S.; Grasso, A.; Grosse-Perdekamp, M.; Grube, B.; Grussenmeyer, T.; Guskov, A.; Haas, F.; Hahne, D.; von Harrach, D.; Hashimoto, R.; Heinsius, F.H.; Herrmann, F.; Hinterberger, F.; Horikawa, N.; d'Hose, N.; Hsieh, C.Yu; Huber, S.; Ishimoto, S.; Ivanov, A.; Ivanshin, Yu.; Iwata, T.; Jahn, R.; Jary, V.; Jorg, P.; Joosten, R.; Kabuss, E.; Ketzer, B.; Khaustov, G.V.; Khokhlov, Yu. A.; Kisselev, Yu.; Klein, F.; Klimaszewski, K.; Koivuniemi, J.H.; Kolosov, V.N.; Kondo, K.; Konigsmann, K.; Konorov, I.; Konstantinov, V.F.; Kotzinian, A.M.; Kouznetsov, O.; Kramer, M.; Kremser, P.; Krinner, F.; Kroumchtein, Z.V.; Kuchinski, N.; Kunne, F.; Kurek, K.; Kurjata, R.P.; Lednev, A.A.; Lehmann, A.; Levillain, M.; Levorato, S.; Lichtenstadt, J.; Longo, R.; Maggiora, A.; Magnon, A.; Makins, N.; Makke, N.; Mallot, G.K.; Marchand, C.; Martin, A.; Marzec, J.; Matousek, J.; Matsuda, H.; Matsuda, T.; Meshcheryakov, G.; Meyer, W.; Michigami, T.; Mikhailov, Yu. V.; Miyachi, Y.; Nagaytsev, A.; Nagel, T.; Nerling, F.; Neyret, D.; Nikolaenko, V.I.; Novy, J.; Nowak, W.D.; Nunes, A.S.; Olshevsky, A.G.; Orlov, I.; Ostrick, M.; Panzieri, D.; Parsamyan, B.; Paul, S.; Peng, J.C.; Pereira, F.; Pesek, M.; Peshekhonov, D.V.; Platchkov, S.; Pochodzalla, J.; Polyakov, V.A.; Pretz, J.; Quaresma, M.; Quintans, C.; Ramos, S.; Regali, C.; Reicherz, G.; Riedl, C.; Rocco, E.; Rossiyskaya, N.S.; Ryabchikov, D.I.; Rychter, A.; Samoylenko, V.D.; Sandacz, A.; Santos, C.; Sarkar, S.; Savin, I.A.; Sbrizzai, G.; Schiavon, P.; Schmidt, K.; Schmieden, H.; Schonning, K.; Schopferer, S.; Selyunin, A.; Shevchenko, O.Yu.; Silva, L.; Sinha, L.; Sirtl, S.; Slunecka, M.; Sozzi, F.; Srnka, A.; Stolarski, M.; Sulc, M.; Suzuki, H.; Szabelski, A.; Szameitat, T.; Sznajder, P.; Takekawa, S.; Wolbeek, J. ter; Tessaro, S.; Tessarotto, F.; Thibaud, F.; Tosello, F.; Tskhay, V.; Uhl, S.; Veloso, J.; Virius, M.; Weisrock, T.; Wilfert, M.; Windmolders, R.; Zaremba, K.; Zavertyaev, M.; Zemlyanichkina, E.; Ziembicki, M.; Zink, A.

    2016-01-01

    New results for the double spin asymmetry $A_1^{\\rm p}$ and the proton longitudinal spin structure function $g_1^{\\rm p}$ are presented. They were obtained by the COMPASS collaboration using polarised 200 GeV muons scattered off a longitudinally polarised NH$_3$ target. The data were collected in 2011 and complement those recorded in 2007 at 160\\,GeV, in particular at lower values of $x$. They improve the statistical precision of $g_1^{\\rm p}(x)$ by about a factor of two in the region $x\\lesssim 0.02$. A next-to-leading order QCD fit to the $g_1$ world data is performed. It leads to a new determination of the quark spin contribution to the nucleon spin, $\\Delta \\Sigma$ ranging from 0.26 to 0.36, and to a re-evaluation of the first moment of $g_1^{\\rm p}$. The uncertainty of $\\Delta \\Sigma$ is mostly due to the large uncertainty in the present determinations of the gluon helicity distribution. A new evaluation of the Bjorken sum rule based on the COMPASS results for the non-singlet structure function $g_1^{\\rm...

  9. The spin-dependent structure function $g_1(x)$ of the proton from polarized deep-inelastic muon scattering

    CERN Document Server

    Adeva, B; Arvidson, A; Badelek, B; Bardin, G; Baum, G; Berglund, P; Betev, L; Birsa, R; De Botton, N R; Bradamante, Franco; Bravar, A; Bressan, A; Bültmann, S; Burtin, E; Crabb, D; Cranshaw, J; Çuhadar-Dönszelmann, T; Dalla Torre, S; Van Dantzig, R; Derro, B R; Deshpande, A A; Dhawan, S K; Dulya, C M; Eichblatt, S; Fasching, D; Feinstein, F; Fernández, C; Forthmann, S; Frois, Bernard; Gallas, A; Garzón, J A; Gilly, H; Giorgi, M A; Görtz, S; Gracia, G; De Groot, N; Haft, K; Von Harrach, D; Hasegawa, T; Hautle, P; Hayashi, N; Heusch, C A; Horikawa, N; Hughes, V W; Igo, G; Ishimoto, S; Iwata, T; Kabuss, E M; Kageya, T; Karev, A G; Ketel, T; Kiryluk, J; Kiselev, Yu F; Krivokhizhin, V G; Kröger, W; Kukhtin, V V; Kurek, K; Kyynäräinen, J; Lamanna, M; Landgraf, U; Le Goff, J M; Lehár, F; de Lesquen, A; Lichtenstadt, J; Litmaath, M; Magnon, A; Mallot, G K; Marie, F; Martin, A; Martino, J; Matsuda, T; Mayes, B W; McCarthy, J S; Medved, K S; Meyer, W T; Van Middelkoop, G; Miller, D; Miyachi, Y; Mori, K; Moromisato, J H; Nassalski, J P; Naumann, Lutz; Niinikoski, T O; Oberski, J; Ogawa, A; Grosse-Perdekamp, M; Pereira, H; Perrot-Kunne, F; Peshekhonov, V D; Pinsky, L; Platchkov, S K; Pló, M; Pose, D; Postma, H; Pretz, J; Puntaferro, R; Rädel, G; Rijllart, A; Reicherz, G; Rodríguez, M; Rondio, Ewa; Roscherr, B; Sabo, I; Saborido, J; Sandacz, A; Savin, I A; Schiavon, R P; Schiller, A; Sichtermann, E P; Simeoni, F; Smirnov, G I; Staude, A; Steinmetz, A; Stiegler, U; Stuhrmann, H B; Szleper, M; Tessarotto, F; Thers, D; Tlaczala, W; Tripet, A; Ünel, G; Velasco, M; Vogt, J; Voss, Rüdiger; Whitten, C; Windmolders, R; Wislicki, W; Witzmann, A; Ylöstalo, J; Zanetti, A M; Zaremba, K

    1997-01-01

    We present a new measurement of the virtual photon proton asymmetry $A_1^{\\rm p}$ from deep inelastic scattering of polarized muons on polarized protons in the kinematic range $0.0008 1$ GeV$^{2}$. A perturbative QCD evolution in next-to-leading order is used to determine $g_1^{\\rm p}(x)$ at a constant $Q^2$. At $Q^{2} = 10$ GeV$^{2}$ we find, in the measured range, $\\int_{0.003}^{0.7} g_{1}^{\\rm p}(x){\\rm d}x = 0.139 \\pm 0.006~({\\rm stat})\\pm 0.008~({\\rm syst)} \\pm 0.006~({\\rm evol})$. The value of the first moment $\\Gamma_{1}^{\\rm p} = \\int_{0}^{1} g_{1}^{\\rm p}(x){\\rm d}x$ of $g_{1}^{\\rm p}$ depends on the approach used to describe the behaviour of $g_{1}^{\\rm p}$ at low $x$. We find that the Ellis-Jaffe sum rule is violated. With our published result for $\\Gamma_{1}^{\\rm d}$ we confirm the Bjorken sum rule with an accuracy of $\\approx 15\\%$ at the one standard deviation level.

  10. Horseshoe Appendix: An Extremely Rare Appendiceal Anomaly.

    Science.gov (United States)

    Singh, Ch Gyan; Nyuwi, Kuotho T; Rangaswamy, Raju; Ezung, Yibenthung S; Singh, H Manihar

    2016-03-01

    Appendiceal anomalies are extremely rare malformations that are usually found incidentally. Agenesis and duplication of the appendix has been well documented however, the cases of horseshoe appendix reported is very limited, only four cases reported so far. Here, we report a four and half-year-old who underwent interval appendectomy. Intraoperatively both the ends of the appendix were found to be communicating with the cecum with two separate base or stump located at a sagital disposal- the so called "horseshoe appendix".

  11. Democratic Republic of the Congo; Selected Issues and Statistical Appendix

    OpenAIRE

    International Monetary Fund

    2003-01-01

    This Selected Issues paper and Statistical Appendix investigates the sources of growth in the Democratic Republic of the Congo (DRC) and evaluates the relative importance of productivity growth and factor accumulation. The analysis is extended to the key sectors of the economy: agriculture, mining, and transport. The paper assesses the DRC’s medium-term growth prospects and compares them with both the post-conflict growth experience to date and the growth objectives of the Poverty Reduction...

  12. Radiation-induced chromosomal hot spots at G 1 and G 2 stages of human lymphocytes in culture

    Directory of Open Access Journals (Sweden)

    Murugesan R

    2003-01-01

    Full Text Available Radiation-induced chromosomal break points in cultured lymphocytes of normal healthy individuals as well as of those with certain genetic disorders are reported to be localized at certain specific loci (hot spots. These reports are based on studies carried out in lymphocytes irradiated at G 1 stage. The present study examines whether the location of hot spots and the frequency seen in cells irradiated at G 1 are similar to those irradiated at G 2 stage of the cell cycle and also tests whether cells of patients exhibit hot spots on irradiation.The results showed that the radiation induced chromosomal break points to be similar in those irradiated are G 1 and G 2 stages of the cell cycle and also that cells of patients exhibited chromosomal hot spots.

  13. Precision measurements of g1 of the proton and the deuteron with 6 GeV electrons

    Energy Technology Data Exchange (ETDEWEB)

    Prok, Yelena; Bosted, Peter; Kvaltine, Nicholas; Adhikari, Krishna; Adikaram-Mudiyanselage, Dasuni; Aghasyan, Mher; Amaryan, Moskov; Anderson, Mark; Anefalos Pereira, Sergio; Avagyan, Harutyun; Baghdasaryan, Hovhannes; Ball, Jacques; Baltzell, Nathan; Battaglieri, Marco; Biselli, Angela; Bono, Jason; Briscoe, William; Brock, Joseph; Brooks, William; Bueltmann, Stephen; Burkert, Volker; Carlin, Christopher; Carman, Daniel; Celentano, Andrea; Chandavar, Shloka; Colaneri, Luca; Cole, Philip; Contalbrigo, Marco; Cortes, Olga; Crabb, Donald; Crede, Volker; D' Angelo, Annalisa; Dashyan, Natalya; De Vita, Raffaella; De Sanctis, Enzo; Deur, Alexandre; Djalali, Chaden; Dodge, Gail; Doughty, David; Dupre, Raphael; El Alaoui, Ahmed; El Fassi, Lamiaa; Elouadrhiri, Latifa; Fedotov, Gleb; Fegan, Stuart; Fersch, Robert; Fleming, Jamie; Forest, Tony; Garcon, Michel; Gevorgyan, Nerses; Ghandilyan, Yeranuhi; Gilfoyle, Gerard; Girod-Gard, Francois-Xavier; Giovanetti, Kevin; Goetz, John; Gohn, Wesley; Gothe, Ralf; Griffioen, Keith; Guegan, Baptiste; Guler, Nevzat; Hafidi, Kawtar; Hanretty, Charles; Harrison, Nathan; Hattawy, Mohammad; Hicks, Kenneth; Ho, Dao; Holtrop, Maurik; Ilieva, Yordanka; Ireland, David; Ishkhanov, Boris; Isupov, Evgeny; Jawalkar, Sucheta; Jiang, Xiaodong; Jo, Hyon-Suk; Joo, Kyungseon; Kalantarians, Narbe; Keith, Christopher; Keller, Daniel; Khandaker, Mahbubul; Kim, Andrey; Kim, Wooyoung; Klein, Andreas; Klein, Franz; Koirala, Suman; Kubarovsky, Valery; Kuhn, Sebastian; Kuleshov, Sergey; Lenisa, Paolo; Livingston, Kenneth; Lu, Haiyun; MacGregor, Ian; Markov, Nikolai; Mayer, Michael; McKinnon, Bryan; Meekins, David; Mineeva, Taisiya; Mirazita, Marco; Mokeev, Viktor; Montgomery, Rachel; MOUTARDE, Herve; Movsisyan, Aram; Munevar Espitia, Edwin; Munoz Camacho, Carlos; Nadel-Turonski, Pawel; Niccolai, Silvia; Niculescu, Gabriel; Niculescu, Maria; Osipenko, Mikhail; Ostrovidov, Alexander; Pappalardo, Luciano; Paremuzyan, Rafayel; Park, K; Peng, Peng; Phillips, J J; Pierce, Joshua; Pisano, Silvia; Pogorelko, Oleg; Pozdniakov, Serguei; Price, John; Procureur, Sebastien; Protopopescu, Dan; Puckett, Andrew; Raue, Brian; Rimal, Dipak; Ripani, Marco; Rizzo, Alessandro; Rosner, Guenther; Rossi, Patrizia; Roy, Priyashree; Sabatie, Franck; Saini, Mukesh; Salgado, Carlos; Schott, Diane; Schumacher, Reinhard; Seder, Erin; Sharabian, Youri; Simonyan, Ani; Smith, Claude; Smith, Gregory; Sober, Daniel; Sokhan, Daria; Stepanyan, Stepan; Stepanyan, Samuel; Strakovski, Igor; Strauch, Steffen; Sytnik, Valeriy; Taiuti, Mauro; Tang, Wei; Tkachenko, Svyatoslav; Ungaro, Maurizio; Vernarsky, Brian; Vlasov, Alexander; Voskanyan, Hakob; Voutier, Eric; Walford, Natalie; Watts, Daniel; Weinstein, Lawrence; Zachariou, Nicholas; Zana, Lorenzo; Zhang, Jixie; Zhao, Bo; Zhao, Zhiwen; Zonta, Irene

    2014-08-01

    The inclusive polarized structure functions of the proton and deuteron, g1p and g1d, were measured with high statistical precision using polarized 6 GeV electrons incident on a polarized ammonia target in Hall B at Jefferson Laboratory. Electrons scattered at lab angles between 18 and 45 degrees were detected using the CEBAF Large Acceptance Spectrometer (CLAS). For the usual DIS kinematics, Q^2>1 GeV^2 and the final-state invariant mass W>2 GeV, the ratio of polarized to unpolarized structure functions g1/F1 is found to be nearly independent of Q^2 at fixed x. Significant resonant structure is apparent at values of W up to 2.3 GeV. In the framework of perturbative QCD, the high-W results can be used to better constrain the polarization of quarks and gluons in the nucleon, as well as high-twist contributions.

  14. Precision measurements of $g_1$ of the proton and the deuteron with 6 GeV electrons

    CERN Document Server

    Prok, Y; Kvaltine, N; Adhikari, K P; Adikaram, D; Aghasyan, M; Amaryan, M J; Anderson, M D; Pereira, S Anefalos; Avakian, H; Baghdasaryan, H; Ball, J; Baltzell, N A; Battaglieri, M; Biselli, A S; Bono, J; Briscoe, W J; Brock, J; Brooks, W K; Bültmann, S; Burkert, V D; Carlin, C; Carman, D S; Celentano, A; Chandavar, S; Colaneri, L; Cole, P L; Contalbrigo, M; Cortes, O; Crabb, D; Crede, V; D'Angelo, A; Dashyan, N; De Vita, R; De Sanctis, E; Deur, A; Djalali, C; Dodge, G E; Doughty, D; Dupre, R; Alaoui, A El; Fassi, L El; Elouadrhiri, L; Fedotov, G; Fegan, S; Fersch, R; Fleming, J A; Forest, T A; Garcon, M; Gevorgyan, N; Ghandilyan, Y; Gilfoyle, G P; Girod, F X; Giovanetti, K L; Goetz, J T; Gohn, W; Gothe, R W; Griffioen, K A; Guegan, B; Guler, N; Haffidi, K; Hanretty, C; Harrison, N; Hattawy, M; Hicks, K; Ho, D; Holtrop, M; Ilieva, Y; Ireland, D G; Ishkhanov, B S; Isupov, E L; Jawalkar, S; Jiang, X; Jo, H S; Joo, K; Kalantarians, N; Keith, C; Keller, D; Khandaker, M; Kim, A; Kim, W; Klein, A; Klein, F J; Koirala, S; Kubarovsky, V; Kuhn, S E; Kuleshov, S V; Lenisa, P; Livingston, K; Lu, H Y; MacGregor, I J D; Markov, N; Mayee, M; McKinnon, B; Meekins, D; Mineeva, T; Mirazita, M; Mokeev, V; Montgomery, R A; Moutarde, H; Movsisyan, A; Munevar, E; Camacho, C Munoz; Nadel-Turonski, P; Niccolai, S; Niculescu, G; Niculescu, I; Osipenko, M; Ostrovidov, A I; Pappalardo, L L; Paremuzyan, R; Park, K; Peng, P; Phillips, J J; Pierce, J; Pisano, S; Pogorelko, O; Pozdniakov, S; Price, J W; Procureur, S; Protopopescu, D; Puckett, A J R; Raue, B A; Rimal, D; Ripani, M; Rizzo, A; Rosner, G; Rossi, P; Roy, P; Sabatié, F; Saini, M S; Salgado, C; Schott, D; Schumacher, R A; Seder, E; Sharabian, Y G; Simonyan, A; Smith, C; Smith, G; Sober, D I; Sokhan, D; Stepanyan, S S; Stepanyan, S; Strakovsky, I I; Strauch, S; Sytnik, V; Taiuti, M; Tang, W; Tkachenko, S; Ungaro, M; Vernarsky, B; Vlassov, A V; Voskanyan, H; Voutier, E; Walford, N K; Watts, D P; Weinstein, L B; Zachariou, N; Zana, L; Zhang, J; Zhao, B; Zhao, Z W; Zonta, I

    2014-01-01

    The inclusive polarized structure functions of the proton and deuteron, g1p and g1d, were measured with high statistical precision using polarized 6 GeV electrons incident on a polarized ammonia target in Hall B at Jefferson Laboratory. Electrons scattered at lab angles between 18 and 45 degrees were detected using the CEBAF Large Acceptance Spectrometer (CLAS). For the usual DIS kinematics, Q^2>1 GeV^2 and the final-state invariant mass W>2 GeV, the ratio of polarized to unpolarized structure functions g1/F1 is found to be nearly independent of Q^2 at fixed x. Significant resonant structure is apparent at values of W up to 2.3 GeV. In the framework of perturbative QCD, the high-W results can be used to better constrain the polarization of quarks and gluons in the nucleon, as well as high-twist contributions.

  15. Improvement of vascular function by acute and chronic treatment with the GPR30 agonist G1 in experimental diabetes mellitus.

    Directory of Open Access Journals (Sweden)

    Zi-lin Li

    Full Text Available The G-protein coupled estrogen receptor 30 (GPR30 is a seven-transmembrane domain receptor that mediates rapid estrogen responses in a wide variety of cell types. This receptor is highly expressed in the cardiovascular system, and exerts vasodilatory effects. The objective of the present study was to investigate the effects of GPR30 on vascular responsiveness in diabetic ovariectomized (OVX rats and elucidate the possible mechanism involved. The roles of GPR30 were evaluated in the thoracic aorta and cultured endothelial cells. The GPR30 agonist G1 induced a dose-dependent vasodilation in the thoracic aorta of the diabetic OVX rats, which was partially attenuated by the nitric oxide synthase (NOS inhibitor, nitro-L-arginine methylester (L-NAME and the GPR30-selective antagonist G15. Dose-dependent vasoconstrictive responses to phenylephrine were attenuated significantly in the rings of the thoracic aorta following the acute G1 administration in the diabetic OVX rats. This effect of G1 was abolished partially by L-NAME and G15. The acute administration of G1 increased significantly the eNOS activity and the concentration of NO in the endothelial cells exposed to high glucose. G1 treatment induced an enhanced endothelium-dependent relaxation to acetylcholine (Ach in the diabetic OVX rats. Further examination revealed that G1 induced vasodilation in the diabetic OVX rats by increasing the phosphorylation of eNOS. These findings provide preliminary evidence that GPR30 activation leads to eNOS activation, as well as vasodilation, to a certain degree and has beneficial effects on vascular function in diabetic OVX rats.

  16. Evaluation of Palm PCRTM G1-12 System: a portable battery-operated PCR thermal cycler

    Directory of Open Access Journals (Sweden)

    Siti Aminah Ahmed

    2016-08-01

    Full Text Available Polymerase chain reaction (PCR is the basis of recombinant and other molecular biological techniques. Availability of cheap and robust PCR platforms enables the tests to be performed easily, even in resource constrained settings. Herein we compared the efficacy of a portable thermal cycler ( Palm PCRTM G1-12 System for rapid DNA amplification against the standard Peltier-based thermal cycler using plasmid DNA and genomic DNA in single and multiplex PCR experiments. Our study revealed that the Palm PCRTM G1-12 System could be a portable DNA amplification system to conduct various molecular techniques, especially in places where resources are limited.

  17. Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system.

    OpenAIRE

    Resnitzky, D; Gossen, M; Bujard, H; Reed, S I

    1994-01-01

    Conditional overexpression of human cyclins B1, D1, and E was accomplished by using a synthetic cDNA expression system based on the Escherichia coli tetracycline repressor. After induction of these cyclins in asynchronous Rat-1 fibroblasts, a decrease in the length of the G1 interval was observed for cyclins D1 and E, consistent with an acceleration of the G1/S phase transition. We observed, in addition, a compensatory lengthening of S phase and G2 so that the mean cell cycle length in popula...

  18. Study of the nucleon spin-dependent structure function $g_1$. A comparison with recent HERMES and COMPASS data

    CERN Document Server

    Kotlorz, Dorota

    2008-01-01

    Predictions for the spin dependent structure function $g_1$ of the nucleon are presented. We use an unified approach incorporating the LO DGLAP evolution and the resummation of double logarithmic terms $ln^2(x)$. We show, that the singular input parametrisation as $x\\to 0$ can be a substitute of the $ln^2(x)$ resummation. An impact of the `more running' coupling is discussed. We determine the contribution to the Bjorken sum rule solving the evolution equation for the truncated moment of $g_1^{NS}$. A comparison with the re-analysed HERMES and COMPASS data is given.

  19. Thermodynamic characterization of the PR-10 allergens Bet v 1, Api g 1 and Dau c 1 and pH-dependence of nApi g 1 and nDau c 1

    NARCIS (Netherlands)

    Bollen, M.A.; Wichers, H.J.; Helsper, J.P.F.G.; Savelkoul, H.F.J.; Boekel, van M.A.J.S.

    2010-01-01

    Natural and recombinant Bet v 1, the major birch pollen allergen, and homologous allergens, Api g 1 and Dau c 1, from celery and carrot, respectively, were studied by CD spectroscopy under conditions of varying denaturant concentration, pH and temperature to determine fundamental thermodynamic param

  20. Base substitution mutations in uridinediphosphate-dependent glycosyltransferase 76G1 gene of Stevia rebaudiana causes the low levels of rebaudioside A: mutations in UGT76G1, a key gene of steviol glycosides synthesis.

    Science.gov (United States)

    Yang, Yong-Heng; Huang, Su-Zhen; Han, Yu-Lin; Yuan, Hai-Yan; Gu, Chun-Sun; Zhao, Yan-Hai

    2014-07-01

    Steviol glycosides, extracted from the leaves of Stevia rebaudiana (Bert) Bertoni, are calorie-free sugar substitute of natural origin with intensely sweet (Boileau et al., 2012). Stevioside and rebaudioside A are the two main kinds of the diterpenic glycosides. We analyzed the concentration of stevioside and rebaudioside A in Stevia leaves of about 500 samples (hybrid progenies) and discovered a mutation plant "Z05" with very low levels of rebaudioside A. Because UGT76G1, a uridinediphosphate-dependent glycosyltransferases, is responsible for the conversion from stevioside to rebaudioside A (Richman et al., 2005), so mutation identification was done by sequencing the candidate gene, UGT76G1. In this study molecular analysis of two strains revealed a heterozygotic nonsense mutation of c.389T > G (p.L121X) in UGT76G1. Meanwhile, we found some amino acid substitutions significant change the protein structure. And the difference of enzyme activity between two strains proved the lack of functionality of UGT76G1 of the mutation "Z05". So the nonsense mutation and amino acid substitution mutation resulted in the low levels of rebaudioside A. PMID:24811677

  1. 考虑驾驶员行为偏好的车辆最优路径选择的G1-TOPSIS法%Vehicle Optimal Path Selection Based on G1-TOPSIS method Considering the Behavior Preference of Driver

    Institute of Scientific and Technical Information of China (English)

    邢建平

    2015-01-01

    The previous optimal path selection of vehicles is mostly considered the shortest path or the least time. However, in practice, there is often a different behavior preference. The different requirements of the driver in the path selection are considered in this article, and the G1 method is used to determine the weight of the influence factors on the path selection. Further the optimal path method based on TOPSIS combining with G1 method is developed, which considers the driver's behavior preference. An example is used to show that the proposed method is effective and practical.%以前的车辆最优路径选择大多是考虑路程最短或时间最少。然而在实际的情形往往是伴随着驾驶员不同的行为偏好。充分考虑了驾驶员在路径选择中的不同要求,将G1法引入到驾驶员路径选择影响因素的权重确定,同时发展出基于G1-TOPSIS法车辆最优路径方法,并通过应用例子说明了方法的有效性和实用性。

  2. Base substitution mutations in uridinediphosphate-dependent glycosyltransferase 76G1 gene of Stevia rebaudiana causes the low levels of rebaudioside A: mutations in UGT76G1, a key gene of steviol glycosides synthesis.

    Science.gov (United States)

    Yang, Yong-Heng; Huang, Su-Zhen; Han, Yu-Lin; Yuan, Hai-Yan; Gu, Chun-Sun; Zhao, Yan-Hai

    2014-07-01

    Steviol glycosides, extracted from the leaves of Stevia rebaudiana (Bert) Bertoni, are calorie-free sugar substitute of natural origin with intensely sweet (Boileau et al., 2012). Stevioside and rebaudioside A are the two main kinds of the diterpenic glycosides. We analyzed the concentration of stevioside and rebaudioside A in Stevia leaves of about 500 samples (hybrid progenies) and discovered a mutation plant "Z05" with very low levels of rebaudioside A. Because UGT76G1, a uridinediphosphate-dependent glycosyltransferases, is responsible for the conversion from stevioside to rebaudioside A (Richman et al., 2005), so mutation identification was done by sequencing the candidate gene, UGT76G1. In this study molecular analysis of two strains revealed a heterozygotic nonsense mutation of c.389T > G (p.L121X) in UGT76G1. Meanwhile, we found some amino acid substitutions significant change the protein structure. And the difference of enzyme activity between two strains proved the lack of functionality of UGT76G1 of the mutation "Z05". So the nonsense mutation and amino acid substitution mutation resulted in the low levels of rebaudioside A.

  3. The MX/G/1 queue with different arrival rates,single vacation and set-up time%具有不同到达率且带有启动时间的单重休假MX/G/1排队

    Institute of Scientific and Technical Information of China (English)

    王莉

    2013-01-01

    研究具有不同到达率且带有启动时间的单重休假Mx/G/1排队模型,给出稳态队长的母函数、等待时间的LST(拉普拉斯-斯蒂尔切斯变换)及其随机分解结果,并推导出忙期、忙循环及在线期均值.%In this paper,a continuous time queue Mx/G/1 with different arrival rates,single vacation and set-up time is considered.The generating function of the steady state queue length,the Laplace-Stieltjes transform(LST) of the waiting time distribution and stochastic decomposition are derived.Furthermore,the mean formulas of the busy period,the whole vacation and on-line period are obtained.

  4. Protective role of mouse IgG1 in cryoglobulinaemia; insights from an animal model and relevance to human pathology.

    Science.gov (United States)

    Chemouny, Jonathan Maurice; Hurtado-Nedelec, Margarita; Flament, Héloïse; Ben Mkaddem, Sanae; Daugas, Eric; Vrtovsnik, François; Berthelot, Laureline; Monteiro, Renato C

    2016-08-01

    Strait et al. described a novel mouse model of cryoglobulinaemia by challenging mice deficient in the immunoglobulin (Ig)G1 subclass (γ1(-) mice) with goat anti-mouse IgD [5]. The phenotype of wild-type mice was not remarkable, whereas γ1(-) mice developed IgG3 anti-goat IgG cryoglobulins as well as severe and lethal glomerulonephritis. Renal phenotype could not be rescued in γ1(-) mice by the deletion of C3, fragment crystalline γ receptor (FcγR) or J chain. On the other hand, early injection of IgG1, IgG2a or IgG2b inhibited the pathogenic effects of IgG3 in an antigen-dependent manner even in the absence of the FcγRIIb, an anti-inflammatory receptor. The authors concluded that the pathogenic role of IgG3 and the protective characteristic of IgG1 in this model were not explained by their abilities to bind to FcRs or effector molecules but are rather due to structural discrepancies enhancing the precipitation properties/solubility of IgG3/IgG1-containing immune complexes. The present article aims to discuss the current knowledge on IgG biology and the properties of IgGs explaining their differential propensity to acquire cryoglobulin activity. PMID:26410885

  5. Experimental infection with Cryptosporidium parvum IIaA21G1R1 subtype in immunosuppressed mice

    Science.gov (United States)

    Cryptosporidium parvum subtype IIaA21G1R1 oocysts were used to infect dexamethasone immunosuppressed N: NIH Swiss mice. Histology showed developmental stages in the duodenum, proximal and distal jejunum, ileum, cecum and colon, with the small intestine remaining infected until day 35 post infection....

  6. The transient M/G/1/0 queue: some bounds and approximations for light traffic with application to reliability

    Directory of Open Access Journals (Sweden)

    J. Ben Atkinson

    1995-01-01

    Full Text Available We consider the transient analysis of the M/G/1/0 queue, for which Pn(t denotes the probability that there are no customers in the system at time t, given that there are n(n=0,1 customers in the system at time 0. The analysis, which is based upon coupling theory, leads to simple bounds on Pn(t for the M/G/1/0 and M/PH/1/0 queues and improved bounds for the special case M/Er/1/0. Numerical results are presented for various values of the mean arrival rate λ to demonstrate the increasing accuracy of approximations based upon the above bounds in light traffic, i.e., as λ→0. An important area of application for the M/G/1/0 queue is as a reliability model for a single repairable component. Since most practical reliability problems have λ values that are small relative to the mean service rate, the approximations are potentially useful in that context. A duality relation between the M/G/1/0 and GI/M/1/0 queues is also described.

  7. Antigenic differences between the EG95-related proteins from Echinococcus granulosus G1 and G6 genotypes: implications for vaccination.

    Science.gov (United States)

    Alvarez Rojas, C A; Gauci, C G; Lightowlers, M W

    2013-02-01

    Cystic echinococcosis caused by Echinococcus granulosus remains an important and neglected issue in public health. The study of the likely efficacy of the currently available EG95 vaccine against other genotypes of the parasite is important to improve the vaccine as a potential tool to be used in control programmes. The recombinant vaccine EG95-1G1 was developed based on the G1 genotype of E. granulosus. Characterization of the eg95 gene family in the G6 genotype by genomic DNA cloning previously produced the first unequivocal information about the composition of the gene family in a different genotype. The information was used in this study to predict and express two EG95-related proteins from the G6 genotype as recombinants, for assessment of their capacity to bind antibodies raised in sheep vaccinated with the EG95-1G1 vaccine. The proteins (EG95-1G6 and EG95-5G6) from the G6 genotype of E. granulosus were unable to bind all the antibodies raised by sheep vaccinated with EG95-1G1. Differences in the amino acid sequence of EG95-related proteins from G6 and likely the differences in the encoded FnIII domain may be responsible for changes in the conformation of these epitopes.

  8. Isolation and characterization of a monoclonal anti CK-2 alpha subunit antibody of the IgG1 subclass

    DEFF Research Database (Denmark)

    Schmidt-Spaniol, I; Boldyreff, B; Issinger, O G

    1992-01-01

    A monoclonal antibody was produced against the recombinant human alpha subunit of CK-2. The antibody was of the IgG1 subclass and it was isolated from serum-free cell culture media and purified by affinity chromatography on Protein G Sepharose. The antibody can be used to detect specifically the ...

  9. Restricted motion of the conserved immunoglobulin G1 N-glycan is essential for efficient FcγRIIIa binding

    Science.gov (United States)

    Subedi, Ganesh P.; Hanson, Quinlin M.; Barb, Adam W.

    2014-01-01

    Summary Immunoglobulin G1(IgG1)-based therapies are widespread and many function through interactions with low-affinity Fc γ receptors (FcγR). N-glycosylation of the IgG1 Fc domain is required for FcγR binding, though it is unclear why. Structures of the FcγR:Fc complex fail to explain this because the FcγR polypeptide does not bind the N-glycan. Here we identify a link between motion of the N-glycan and Fc:FcγRIIIa affinity that explains the N-glycan requirement. Fc F241 and F243 mutations decreased the N-glycan/polypeptide interaction and increased N-glycan mobility. The affinity of the Fc mutants for FcγRIIIa was directly proportional to the degree of glycan restriction (R2=0.82). The IgG1 Fc K246F mutation stabilized the N-glycan and enhanced affinity for FcγRIIIa. Allosteric modulation of a protein/protein interaction represents a previously undescribed role for N-glycans in biology. Conserved features suggesting a similar N-glycan/aromatic interaction were also found in IgD, E and M, but not A. PMID:25199692

  10. G0/G1 switch gene-2 regulates human adipocyte lipolysis by affecting activity and localization of adipose triglyceride lipase

    NARCIS (Netherlands)

    Schweiger, M.; Paar, M.; Eder, C.; Brandis, J.; Moser, E.; Gorkiewisz, G.; Grond, S.; Radner, F.P.W.; Cerk, I.; Cornaciu, I.; Oberer, M.; Kersten, A.H.; Zechner, R.; Zimmermann, M.B.; Lass, A.

    2012-01-01

    The hydrolysis of triglycerides in adipocytes, termed lipolysis, provides free fatty acids as energy fuel. Murine lipolysis largely depends on the activity of adipose triglyceride lipase (ATGL)5, which is regulated by two proteins annotated as comparative gene identification-58 (CGI-58) and G0/G1 sw

  11. Waste-Management Education and Research Consortium (WERC) annual progress report, 1991--1992. Appendixes

    Energy Technology Data Exchange (ETDEWEB)

    1992-04-07

    This report contains the following appendices: Appendix A - Requirements for Undergraduate Level; Appendix B - Requirements for Graduate Level; Appendix C - Graduate Degree In Environmental Engineering; Appendix D - Non-degree Certificate Program; Appendix E - Curriculum for Associate Degree Program; Appendix F - Curriculum for NCC Program; Appendix G - Information 1991 Teleconference Series; Appendix H - Information on 1992 Teleconference Series; Appendix I - WERC interactive Television Courses; Appendix J - WERC Research Seminar Series; Appendix K - Sites for Hazardous/Radioactive Waste Management Series; Appendix L- Summary of Technology Development of the Second Year; Appendix M - List of Major Publications Resulting from WERC; Appendix N - Types of Equipment at WERC Laboratories.

  12. Selected working papers

    International Nuclear Information System (INIS)

    The following working papers are included in this report: WP 2002-2, Dictionary Restructuring; WP-2002-4, Page numbers for REFERENCE (CP-C/285); WP-2002-5, Correlation quantities; WP 2002-8, Proposed data heading EN-CM-TOT (memo CP-A/121); WP 2002-9, Proposed high energy quantities (memo CP-A/123); WP 2002-11, Use of nuclide codes in SF 7 (memo CP-C/302); WP 2002-12, Redundant coding, new data heading PART-OUT; WP 2002-16, Zeros in error field (CP-C/306); WP 2002-17, Multiple appearance of the first Reference in EXFOR; WP 2002-18 + Add., EXFOR master file comparisons; WP 2002-19, Measures of Security at the NDS Open Area for EXFOR; WP 2002-20, New and revised entries received at NDS; WP 2002-21, EXFOR transmissions (NNDC); WP 2002-22, CINDA statistics (NNDC); WP 2002-24, CINDA batch exchange information (NDS); WP 2002-25, Journal coverage for CINDA; WP 2002-26, EXFOR-relational as multi-platform database (V. Zerkin); WP 2002-27, Completeness of EXFOR compil. as indexed by CINDA; WP 2002-28, Future NRDC Cooperation on CINDA: see Appendix 9; WP 2002-31, Development of Web Editor for Charged-Particle Nuclear Reaction Data (N. Otuka, H. Noto, A. Ohnishi, K. Kato). The following other papers, or the memos of which they consist, are available from the IAEA Nuclear Data Section: WP 2002-1, Actions of previous meetings (2002, 2001) see INDC(NDS)-427, pp.20-26, and INDC(NDS)-418, pp.26-31; WP 2002-3, Units for particle and product yields: see memos CP-C/294, 286; WP 2002-6, 4-momentum transfer and mom.distr.data: see memos CP-C/295 and CP-D/330; WP 2002-7, Several 'straightforward' new quantities: see memos CP-/C-291, 298 and CP-A/118; WP 2002-10, Quantities proposed by JCPRG (see memos DP-D/337, CP-E/004, 003); WP 2002-13, Clarifications on Product Yields and Thick Target Yields (see memo CP-D/332); WP 2002-14, Clarifications on Polarization quantities (see memo CP-D/320); WP 2002-15, New Legendre polynomial modifier proposed (see CP-C/305); WP 2002-23, see memo 4C-4

  13. Genome-Wide Evolutionary Analyses of G1P[8] Strains Isolated Before and After Rotavirus Vaccine Introduction

    Science.gov (United States)

    Zeller, Mark; Donato, Celeste; Trovão, Nídia Sequeira; Cowley, Daniel; Heylen, Elisabeth; Donker, Nicole C.; McAllen, John K.; Akopov, Asmik; Kirkness, Ewen F.; Lemey, Philippe; Van Ranst, Marc; Matthijnssens, Jelle; Kirkwood, Carl D.

    2015-01-01

    Rotaviruses are the most important etiological agent of acute gastroenteritis in young children worldwide. Among the first countries to introduce rotavirus vaccines into their national immunization programs were Belgium (November 2006) and Australia (July 2007). Surveillance programs in Belgium (since 1999) and Australia (since 1989) offer the opportunity to perform a detailed comparison of rotavirus strains circulating pre- and postvaccine introduction. G1P[8] rotaviruses are the most prominent genotype in humans, and a total of 157 G1P[8] rotaviruses isolated between 1999 and 2011 were selected from Belgium and Australia and their complete genomes were sequenced. Phylogenetic analysis showed evidence of frequent reassortment among Belgian and Australian G1P[8] rotaviruses. Although many different phylogenetic subclusters were present before and after vaccine introduction, some unique clusters were only identified after vaccine introduction, which could be due to natural fluctuation or the first signs of vaccine-driven evolution. The times to the most recent common ancestors for the Belgian and Australian G1P[8] rotaviruses ranged from 1846 to 1955 depending on the gene segment, with VP7 and NSP4 resulting in the most recent estimates. We found no evidence that rotavirus population size was affected after vaccine introduction and only six amino acid sites in VP2, VP3, VP7, and NSP1 were identified to be under positive selective pressure. Continued surveillance of G1P[8] strains is needed to determine long-term effects of vaccine introductions, particularly now rotavirus vaccines are implemented in the national immunization programs of an increasing number of countries worldwide. PMID:26254487

  14. Genome-Wide Evolutionary Analyses of G1P[8] Strains Isolated Before and After Rotavirus Vaccine Introduction.

    Science.gov (United States)

    Zeller, Mark; Donato, Celeste; Trovão, Nídia Sequeira; Cowley, Daniel; Heylen, Elisabeth; Donker, Nicole C; McAllen, John K; Akopov, Asmik; Kirkness, Ewen F; Lemey, Philippe; Van Ranst, Marc; Matthijnssens, Jelle; Kirkwood, Carl D

    2015-09-01

    Rotaviruses are the most important etiological agent of acute gastroenteritis in young children worldwide. Among the first countries to introduce rotavirus vaccines into their national immunization programs were Belgium (November 2006) and Australia (July 2007). Surveillance programs in Belgium (since 1999) and Australia (since 1989) offer the opportunity to perform a detailed comparison of rotavirus strains circulating pre- and postvaccine introduction. G1P[8] rotaviruses are the most prominent genotype in humans, and a total of 157 G1P[8] rotaviruses isolated between 1999 and 2011 were selected from Belgium and Australia and their complete genomes were sequenced. Phylogenetic analysis showed evidence of frequent reassortment among Belgian and Australian G1P[8] rotaviruses. Although many different phylogenetic subclusters were present before and after vaccine introduction, some unique clusters were only identified after vaccine introduction, which could be due to natural fluctuation or the first signs of vaccine-driven evolution. The times to the most recent common ancestors for the Belgian and Australian G1P[8] rotaviruses ranged from 1846 to 1955 depending on the gene segment, with VP7 and NSP4 resulting in the most recent estimates. We found no evidence that rotavirus population size was affected after vaccine introduction and only six amino acid sites in VP2, VP3, VP7, and NSP1 were identified to be under positive selective pressure. Continued surveillance of G1P[8] strains is needed to determine long-term effects of vaccine introductions, particularly now rotavirus vaccines are implemented in the national immunization programs of an increasing number of countries worldwide. PMID:26254487

  15. Improved ENSO simulation from climate system model FGOALS-g1.0 to FGOALS-g2

    Science.gov (United States)

    Chen, Lin; Yu, Yongqiang; Zheng, Weipeng

    2016-10-01

    This study presents an overview of the improvement in the simulation of El Niño-Southern Oscillation (ENSO) in the latest generation of the Institute of Atmospheric Physics' coupled general circulation model (CGCM), the Flexible Global Ocean-Atmosphere-Land System model Grid-point Version 2 (FGOALS-g2; hereafter referred to as "g2") from its predecessor FGOALS-g1.0 (referred to as "g1"), including the more realistic amplitude, irregularity, and ENSO cycle. The changes have been analyzed quantitatively based on the Bjerknes stability index, which serves as a measure of ENSO growth rate. The improved simulation of ENSO amplitude is mainly due to the reasonable representation of the thermocline and thermodynamic feedbacks: On the one hand, the deeper mean thermocline results in a weakened thermocline response to the zonal wind stress anomaly, and the looser vertical stratification of mean temperature leads to a weakened response of anomalous subsurface temperature to anomalous thermocline depth, both of which cause the reduced thermocline feedback in g2; on the other hand, the alleviated cold bias of mean sea surface temperature leads to more reasonable thermodynamic feedback in g2. The regular oscillation of ENSO in g1 is associated with its unsuccessful representation of the role of atmospheric noise over the western-central equatorial Pacific (WCEP) in triggering ENSO events, which arises from the weak synoptic-intraseasonal variability of zonal winds over the WCEP in g1. The asymmetric transition of ENSO in g1 is attributed to the asymmetric effect of thermocline feedback, which is due to the annual cycle of mean upwelling in the eastern Pacific. This study highlights the great impact of improving the representation of mean states on the improved simulation of air-sea feedback processes and ultimately more reasonable depiction of ENSO behaviors in CGCMs.

  16. Overestimated Oncologic Significance of Lymph Node Metastasis in G1 Nonfunctioning Neuroendocrine Tumor in the Left Side of the Pancreas.

    Science.gov (United States)

    Yoo, Young Jin; Yang, Seok Jeong; Hwang, Ho Kyoung; Kang, Chang Moo; Kim, Hogeun; Lee, Woo Jung

    2015-09-01

    Recent studies have expounded on the oncologic significance of lymph node metastasis in nonfunctioning (NF) neuroendocrine tumors (NETs) of the pancreas and suggest regional lymph node dissection for treating pancreatic NET. We tested this recommendation in NF pancreatic NET-G1, as these tumors are generally small and suitable for function-preserving minimally invasive pancreatectomy.From January 2005 to December 2014, medical records of patients who underwent pancreatectomy for pathologically confirmed NF NET-G1 of the left side of the pancreas were retrospectively reviewed. Oncologic outcomes were compared between limited pancreatectomy and distal pancreatosplenectomy.Thirty-five patients (14 males and 21 females) with a mean age of 55.9 ± 11.4 years were enrolled in this study. Six patients (17.1%) underwent distal pancreatosplenectomy. Limited pancreatectomies comprised 15 spleen-preserving distal pancreatectomies (42.8%), 10 enucleations (28.6%), and 4 central pancreatectomies (11.4%). Lymph node metastasis was not found in 6 patients who underwent distal pancreatectomy with a splenectomy; meanwhile, the others were regarded as pNx since no lymph node retrieval was attempted during the limited pancreatectomy. Overall disease-free survival was 36.5 months (95% confidence interval [CI]: 25.9-47.1) and no tumor-related mortality was noted. Minimally invasive pancreatectomy (P = 0.557) and limited pancreatectomy (P = 0.758) showed no adverse impact in treating NF NET-G1 of the left side of the pancreas.The oncologic significance of lymph node metastasis is overestimated in NF NET-G1 of the left side of the pancreas. Routine conventional distal pancreatosplenectomy to retrieve regional lymph nodes may be too excessive in treating NF NET-G1 of the distal pancreas.

  17. Piperine causes G1 phase cell cycle arrest and apoptosis in melanoma cells through checkpoint kinase-1 activation.

    Directory of Open Access Journals (Sweden)

    Neel M Fofaria

    Full Text Available In this study, we determined the cytotoxic effects of piperine, a major constituent of black and long pepper in melanoma cells. Piperine treatment inhibited the growth of SK MEL 28 and B16 F0 cells in a dose and time-dependent manner. The growth inhibitory effects of piperine were mediated by cell cycle arrest of both the cell lines in G1 phase. The G1 arrest by piperine correlated with the down-regulation of cyclin D1 and induction of p21. Furthermore, this growth arrest by piperine treatment was associated with DNA damage as indicated by phosphorylation of H2AX at Ser139, activation of ataxia telangiectasia and rad3-related protein (ATR and checkpoint kinase 1 (Chk1. Pretreatment with AZD 7762, a Chk1 inhibitor not only abrogated the activation of Chk1 but also piperine mediated G1 arrest. Similarly, transfection of cells with Chk1 siRNA completely protected the cells from G1 arrest induced by piperine. Piperine treatment caused down-regulation of E2F1 and phosphorylation of retinoblastoma protein (Rb. Apoptosis induced by piperine was associated with down-regulation of XIAP, Bid (full length and cleavage of Caspase-3 and PARP. Furthermore, our results showed that piperine treatment generated ROS in melanoma cells. Blocking ROS by tiron protected the cells from piperine mediated cell cycle arrest and apoptosis. These results suggest that piperine mediated ROS played a critical role in inducing DNA damage and activation of Chk1 leading to G1 cell cycle arrest and apoptosis.

  18. Appendix: proof of the Uniformity Conjecture

    CERN Document Server

    Baldassarri, Francesco

    2011-01-01

    This paper originated as an appendix to the paper "Topology and Geometry of the Berkovich Ramification Locus for Rational Functions, II" by Xander Faber arXiv:1104.0943v2 [math.NT]. It may however be read independently. We prove a variant of the p-adic Rolle theorem, via the theory of the radius of convergence of p-adic connections and the theory of semistable reduction of p-adic curves. We carefully compare the present author's notion [Inv. Math. 182 (2010)] of radius of convergence, of a connection on a p-adic curve X, normalized by the choice of a semistable model of X, with Kedlaya's intrinsic generic radius of convergence of a differential module [Def. 9.4.7 in p-adic Differential Equations, Cambridge Studies in Adv. Math., vol. 125 (2010)].

  19. G1 condition for C-curves and application in surface modeling%C-曲线间G1拼接条件及在曲面造型中的应用

    Institute of Scientific and Technical Information of China (English)

    王刘强; 刘旭敏

    2007-01-01

    C-B样条无法精确表示半圆弧和半椭圆弧,在对C-B样条曲线和C-Bézier曲线基函数及端点特性分析的基础上,通过增加控制顶点使C-B样条曲线通过控制多边形的首末顶点并与首末边相切,给出了C-B样条曲线和C-Bézier曲线间G1拼接条件;利用C-Bézier曲线表示半圆弧和半椭圆弧,并与C-B样条曲线进行G1拼接,从而解决了C-B样条曲面造型中半圆弧和半椭圆弧的表示问题.

  20. 批量到达带启动时间的单重休假M/G/1排队系统%Single Vacation and Set-Up Time of M/G/1 Queueing System with Batch Arrival

    Institute of Scientific and Technical Information of China (English)

    高娃

    2005-01-01

    本文研究批量到达带启动时间的单重休假的M/G/1排队系统,给出稳态队长的母函数和等待时间分布的LST及其它们的随机分解结果,推导出忙期、闲期和线期母函数和均值.

  1. Streptococcus milleri in the appendix.

    Science.gov (United States)

    Poole, P M; Wilson, G

    1977-10-01

    The appendix was investigated as a possible habitat of Streptococcus milleri. Both normal and inflamed appendices were examined and the isolation rates compared. S. milleri was present in a quarter of the normal appendices and more than half of those associated with apendicitis--a difference that was statistically highly significant. The isolation rates throughout were indepencent of age. There was a pronounced connection between the presence of S. milleri in the appendix and the purulent manifestations of appendicitis. S. milleri was isolated from other abdominal sites associated with appendicitis. The frequency of isolation was increased by culture in an enrichment broth containing nalidixic acid and sulphadimidine.

  2. 26 CFR 1.1033(g)-1 - Condemnation of real property held for productive use in trade or business or for investment.

    Science.gov (United States)

    2010-04-01

    ... productive use in trade or business or for investment. 1.1033(g)-1 Section 1.1033(g)-1 Internal Revenue... Nontaxable Exchanges § 1.1033(g)-1 Condemnation of real property held for productive use in trade or business... advertising displays as real property—(1) In general. Under section 1033(g)(3) of the Code, a taxpayer...

  3. The expression and functional activity of membrane-bound human leukocyte antigen-G1 are influenced by the 3'-untranslated region

    DEFF Research Database (Denmark)

    Svendsen, Signe Goul; Hantash, Basil M; Zhao, Longmei;

    2013-01-01

    , and graft rejection after organ transplantation. We have investigated the effect of HLA-G polymorphism in the 3'UTR on the processing and stability of the membrane-bound HLA-G1 (mHLA-G1) isoform, as well as its functional significance. Different HLA-G1 cDNA sequences were transduced into the human K562 cell...

  4. Future-oriented power distribution grid. Handling of up to 50% fluctuating power generation in 2020. Issue paper - working group G1; Denmark. Smart Grid Network; Fremtidssikring af elnettet. Haandtering af op til 50 pct. fluktuerende elproduktion i 2020. Issue paper, arbejdsgruppe G1

    Energy Technology Data Exchange (ETDEWEB)

    Soendergren, C.; Norsk Jensen, A. (Dansk Energi, Frederiksberg (Denmark)); OEstergaard, J. (Danmarks Tekniske Univ.. DTU Elektro, Kgs. Lyngby (Denmark)) (and others)

    2011-07-01

    The Smart Grid Network was established in 2010 by the Danish climate and energy minister tasked with developing recommendations for future actions and initiatives that make it possible to handle up to 50% electricity from wind energy in the power system in 2020. The task of working group 1 of the Smart Grid Network is stated as: ''The group will, building on existing surveys and analyses of structures and constraints in the Danish electricity system 2020, define concrete ideas on the technical and system development needs for the electricity supply system when the goal of intelligent integration of 50 percent power generated by renewable energy must be met. The group should come up with concrete suggestions on how to make the power system future-oriented, such as new network and management architectures, more measuring equipment, optical sensors, and new computer and communications systems that can monitor, control and operate the power system with the aim that the new intelligence can contribute to a reliable and cost-effective power system that can handle the deployment of renewable energy''. The working group has focused on a general description of the whole overall system needs and more specifically on the power grid and its management and control. (LN)

  5. Isolation and partial identification of eight endogenous G1 inhibitors of JB-1 ascites tumor cell proliferation.

    Science.gov (United States)

    Barfod, N M

    1982-06-01

    Eight endogenous G1 inhibitors of the proliferation of JB-1 ascites tumor cells have been isolated and characterized. The activity of the inhibitors has been analyzed on synchronized JB-1 (murine plasmacytoma) and L1A2 (murine sarcoma) cells in vitro using flow cytometry. The purified inhibitors have been tested for in vivo activity on partially synchronized JB-1 and L1A2 ascites tumors in situ. Four of the inhibitors exhibited a high degree of cell specificity (chalone-like inhibitors) and were chemically related, whereas the other four showed no cell specificity. In most extractions, the amount of cell-specific activity is more than 50% of the total G1-inhibitory activity. Most of the inhibitors are low-molecular-weight peptides and glycopeptides.

  6. Reassortment of Human and Animal Rotavirus Gene Segments in Emerging DS-1-Like G1P[8] Rotavirus Strains

    Science.gov (United States)

    Komoto, Satoshi; Tacharoenmuang, Ratana; Guntapong, Ratigorn; Ide, Tomihiko; Tsuji, Takao; Yoshikawa, Tetsushi; Tharmaphornpilas, Piyanit; Sangkitporn, Somchai; Taniguchi, Koki

    2016-01-01

    The emergence and rapid spread of novel DS-1-like G1P[8] human rotaviruses in Japan were recently reported. More recently, such intergenogroup reassortant strains were identified in Thailand, implying the ongoing spread of unusual rotavirus strains in Asia. During rotavirus surveillance in Thailand, three DS-1-like intergenogroup reassortant strains having G3P[8] (RVA/Human-wt/THA/SKT-281/2013/G3P[8] and RVA/Human-wt/THA/SKT-289/2013/G3P[8]) and G2P[8] (RVA/Human-wt/THA/LS-04/2013/G2P[8]) genotypes were identified in fecal samples from hospitalized children with acute gastroenteritis. In this study, we sequenced and characterized the complete genomes of strains SKT-281, SKT-289, and LS-04. On whole genomic analysis, all three strains exhibited unique genotype constellations including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 for strains SKT-281 and SKT-289, and G2-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 for strain LS-04. Except for the G genotype, the unique genotype constellation of the three strains (P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2) is commonly shared with DS-1-like G1P[8] strains. On phylogenetic analysis, nine of the 11 genes of strains SKT-281 and SKT-289 (VP4, VP6, VP1-3, NSP1-3, and NSP5) appeared to have originated from DS-1-like G1P[8] strains, while the remaining VP7 and NSP4 genes appeared to be of equine and bovine origin, respectively. Thus, strains SKT-281 and SKT-289 appeared to be reassortant strains as to DS-1-like G1P[8], animal-derived human, and/or animal rotaviruses. On the other hand, seven of the 11 genes of strain LS-04 (VP7, VP6, VP1, VP3, and NSP3-5) appeared to have originated from locally circulating DS-1-like G2P[4] human rotaviruses, while three genes (VP4, VP2, and NSP1) were assumed to be derived from DS-1-like G1P[8] strains. Notably, the remaining NSP2 gene of strain LS-04 appeared to be of bovine origin. Thus, strain LS-04 was assumed to be a multiple reassortment strain as to DS-1-like G1P[8], locally circulating

  7. sHLA-G1 and HLA-G5 levels are decreased in Tunisian women with multiple abortion.

    Science.gov (United States)

    Zidi, Inès; Rizzo, Roberta; Bouaziz, Aicha; Laaribi, Ahmed Baligh; Zidi, Nour; Di Luca, Dario; Tlili, Henda; Bortolotti, Daria

    2016-04-01

    Pregnancy is associated with increased levels of soluble (s) human leukocyte antigen (HLA)-G molecules, while during abortion these molecules are decreased. To date, little is known about the role of sHLA-G isoforms during abortion. In this study, we investigated the levels of total sHLA-G and its isoforms: HLA-G1 (membrane shedded isoform) and alternative spliced HLA-G5 in plasma samples obtained from 55 women who had experienced spontaneous abortion, 108 pregnant healthy women and 56 non pregnant healthy women. We found that pregnant women exhibited higher amounts of sHLA-G compared to either non pregnant women or women with abortion. Among women who had experienced spontaneous abortion, women with recurrent abortions (RSA) had lower sHLA-G than women with only one abortion. In particular, RSA women were characterized by the absence of sHLA-G1 isoform, suggesting a possible implication in abortion event. PMID:26812178

  8. X-ray Spectral Variations in the Youngest Galactic Supernova Remnant G1.9+0.3

    CERN Document Server

    Reynolds, S P; Green, D A; Hwang, U; Harrus, I; Petre, R

    2009-01-01

    The discovery of the youngest Galactic supernova remnant (SNR) G1.9+0.3 has allowed a look at a stage of SNR evolution never before observed. We analyze the 50 ks Chandra observation with particular regard to spectral variations. The very high column density ($N_H \\sim 6 \\times 10^{22}$ cm$^{-2}$) implies that dust scattering is important, and we use a simple scattering model in our spectral analysis. The integrated X-ray spectrum of G1.9+0.3 is well described by synchrotron emission from a power-law electron distribution with an exponential cutoff. Using our measured radio flux and including scattering effects, we find a rolloff frequency of $5.4 (3.0, 10.2) \\times 10^{17}$ Hz ($h \

  9. Crystal Structures of Glycosyltransferase UGT78G1 Reveal the Molecular Basis for Glycosylation and Deglycosylation of (Iso)flavonoids

    Energy Technology Data Exchange (ETDEWEB)

    Modolo, Luzia V.; Li, Lenong; Pan, Haiyun; Blount, Jack W.; Dixon, Richard A.; Wang, Xiaoqiang; (SRNF)

    2010-09-21

    The glycosyltransferase UGT78G1 from Medicago truncatula catalyzes the glycosylation of various (iso)flavonoids such as the flavonols kaempferol and myricetin, the isoflavone formononetin, and the anthocyanidins pelargonidin and cyanidin. It also catalyzes a reverse reaction to remove the sugar moiety from glycosides. The structures of UGT78G1 bound with uridine diphosphate or with both uridine diphosphate and myricetin were determined at 2.1 {angstrom} resolution, revealing detailed interactions between the enzyme and substrates/products and suggesting a distinct binding mode for the acceptor/product. Comparative structural analysis and mutagenesis identify glutamate 192 as a key amino acid for the reverse reaction. This information provides a basis for enzyme engineering to manipulate substrate specificity and to design effective biocatalysts with glycosylation and/or deglycosylation activity.

  10. Reassortment of Human and Animal Rotavirus Gene Segments in Emerging DS-1-Like G1P[8] Rotavirus Strains.

    Directory of Open Access Journals (Sweden)

    Satoshi Komoto

    Full Text Available The emergence and rapid spread of novel DS-1-like G1P[8] human rotaviruses in Japan were recently reported. More recently, such intergenogroup reassortant strains were identified in Thailand, implying the ongoing spread of unusual rotavirus strains in Asia. During rotavirus surveillance in Thailand, three DS-1-like intergenogroup reassortant strains having G3P[8] (RVA/Human-wt/THA/SKT-281/2013/G3P[8] and RVA/Human-wt/THA/SKT-289/2013/G3P[8] and G2P[8] (RVA/Human-wt/THA/LS-04/2013/G2P[8] genotypes were identified in fecal samples from hospitalized children with acute gastroenteritis. In this study, we sequenced and characterized the complete genomes of strains SKT-281, SKT-289, and LS-04. On whole genomic analysis, all three strains exhibited unique genotype constellations including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 for strains SKT-281 and SKT-289, and G2-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 for strain LS-04. Except for the G genotype, the unique genotype constellation of the three strains (P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 is commonly shared with DS-1-like G1P[8] strains. On phylogenetic analysis, nine of the 11 genes of strains SKT-281 and SKT-289 (VP4, VP6, VP1-3, NSP1-3, and NSP5 appeared to have originated from DS-1-like G1P[8] strains, while the remaining VP7 and NSP4 genes appeared to be of equine and bovine origin, respectively. Thus, strains SKT-281 and SKT-289 appeared to be reassortant strains as to DS-1-like G1P[8], animal-derived human, and/or animal rotaviruses. On the other hand, seven of the 11 genes of strain LS-04 (VP7, VP6, VP1, VP3, and NSP3-5 appeared to have originated from locally circulating DS-1-like G2P[4] human rotaviruses, while three genes (VP4, VP2, and NSP1 were assumed to be derived from DS-1-like G1P[8] strains. Notably, the remaining NSP2 gene of strain LS-04 appeared to be of bovine origin. Thus, strain LS-04 was assumed to be a multiple reassortment strain as to DS-1-like G1P[8], locally

  11. The ($G' /G, 1/G$)-expansion method for solving nonlinear space–time fractional differential equations

    Indian Academy of Sciences (India)

    EMRULLAH YA¸SAR; ILKER BURAK GIRESUNLU

    2016-08-01

    In this work, we present ($G' /G, 1/G$)-expansion method for solving fractional differential equations based on a fractional complex transform. We apply this method for solving space–time fractional Cahn--Allen equation and space--time fractional Klein–Gordon equation. The fractional derivatives are described in the sense of modified Riemann--Lioville. As a result of some exact solution in the form of hyperbolic, trigonometric and rational solutions are deduced. The obtained solutions may be used for explaining of some physical problems.The($G' /G, 1/G$)-expansion method has a wider applicability for nonlinear equations. We have verified all the obtained solutions with the aid of Maple.

  12. Mainstream cigarette smoke exposure alters cytochrome P4502G1 expression in F344 rat olfactory mucosa

    International Nuclear Information System (INIS)

    Inhalation of mainstream cigarette smoke (MCS) by rats results in multifocal rhinitis, mucous hypersecretion, nasal epithelial hyperplasia and metaplasia, and focal olfactory mucosal atrophy. In humans, cigarette smoking causes long-term, dose-related alterations in olfactory function in both current and former smokers. An olfactory-specific cytochrome P450 has been identified in rabbits and rats. The presence of olfactory-specific P450s, as well as relatively high levels of other biotransformation enzymes, such as NADPH-cytochrome P450 reductase and UDP-glucuronosyl transferase, in the olfactory neuroepithelium suggest that these enzyme systems may play a role in olfaction. This hypothesis is strengthened by the observation that, in rats, the temporal gene activation of P4502G1 coincides with the postnatal increase in the sensitivity of olfactory response to odorants. The purpose of this investigation was to examine the effect of MCS exposure on P4502G1 protein expression

  13. A novel peptide sansalvamide analogue inhibits pancreatic cancer cell growth through G0/G1 cell-cycle arrest

    International Nuclear Information System (INIS)

    Patients with pancreatic cancer have little hope for cure because no effective therapies are available. Sansalvamide A is a cyclic depsipeptide produced by a marine fungus. We investigated the effect of a novel sansalvamide A analogue on growth, cell-cycle phases, and induction of apoptosis in human pancreatic cancer cells in vitro. The sansalvamide analogue caused marked time- and concentration-dependent inhibition of DNA synthesis and cell proliferation of two human pancreatic cancer cell lines (AsPC-1 and S2-013). The analogue induced G0/G1 phase cell-cycle arrest and morphological changes suggesting induction of apoptosis. Apoptosis was confirmed by annexin V binding. This novel sansalvamide analogue inhibits growth of pancreatic cancer cells through G0/G1 arrest and induces apoptosis. Sansalvamide analogues may be valuable for the treatment of pancreatic cancer

  14. Evolutionarily conserved transcription factor Apontic controls the G1/S progression by inducing cyclin e during eye development

    KAUST Repository

    Liu, Qingxin

    2014-06-16

    During Drosophila eye development, differentiation initiates in the posterior region of the eye disk and progresses anteriorly as a wave marked by the morphogenetic furrow (MF), which demarcates the boundary between anterior undifferentiated cells and posterior differentiated photoreceptors. However, the mechanism underlying the regulation of gene expression immediately before the onset of differentiation remains unclear. Here, we show that Apontic (Apt), which is an evolutionarily conserved transcription factor, is expressed in the differentiating cells posterior to the MF. Moreover, it directly induces the expression of cyclin E and is also required for the G1-to-S phase transition, which is known to be essential for the initiation of cell differentiation at the MF. These observations identify a pathway crucial for eye development, governed by a mechanism in which Cyclin E promotes the G1-to-S phase transition when regulated by Apt.

  15. Toxocara canis glycans influence antigen recognition by mouse IgG1 and IgM antibodies.

    Science.gov (United States)

    Długosz, Ewa; Wiśniewski, Marcin

    2016-01-01

    The impact of sugar moieties of Toxocara canis glycoprotein antigens on their recognition by infected mouse antibodies was investigated in this study. Native TES and recombinant Toxocara mucins generated in Pichia pastoris yeast as well as their deglycosylated forms were used in ELISA. TES and recombinant mucins were equally recognized by T. canis infected mouse IgG1 antibodies. IgM immunoglobulins predominantly recognized TES antigens. Among mucins recognition of Tc-MUC-4 was the most significant. Deglycosylation of antigens resulted in significant loss of IgM and IgG1 reactivity to TES, mucins, Tc-MUC-3 and Tc-MUC-4. The presence of sugar moieties had no influence on IgE binding to native or recombinant T. canis antigens. Our results suggest that glycans are involved in epitope formation what should be taken into consideration in production of recombinant helminth antigens for diagnostic purposes. PMID:26751891

  16. Detailed petrographic descriptions and microprobe data for tertiary silicic volcanic rocks in drill hole USW G-1, Yucca Mountain, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Caporuscio, F.A.; Warren, R.G.; Broxton, D.E.

    1985-12-01

    This report contains detailed petrographic descriptions of 74 thin sections from drill hole USW G-1 at Yucca Mountain, Nevada. These descriptions are keyed to the distinctions between devitrified, vitrophyre, vitric, and zeolitized intervals below the Topopah Spring Member repository horizon. The petrographic features of the zeolitized intervals down through the Crater Flat tuff, as well as the sorption properties determined from these intervals, suggest that these zeolite occurrences may each have comparable sorptive capability.

  17. Sequence analysis of VP4 genes of wild type and culture adapted human rotavirus G1P[8] strains

    Institute of Scientific and Technical Information of China (English)

    Ritu Arora; Ganesh S Dhale; Pooja R Patil; Shobha D Chitambar

    2011-01-01

    Objective:To conduct a comparative analysis of the VP4gene sequences of Indian wild type (06361,0613158, 061060and0715880) and cell culture adapted (06361-CA, 0613158-CA, 061060-CAand0715880-CA) G1P[8] rotavirus strains.Methods: Full-length VP4 genes of each of the four wild type G1P[8] rotavirus strains and their cell culture adapted counterparts displaying consistent cytopathic effect were subjected toRT-PCRamplification and nucleotide sequencing. Results: All four cell culture adaptedG1P[8]rotavirus strains showed nucleotide and amino acid substitutions in theVP4 gene as compared to their wild type strains. The number of substitutions however, varied from1-64and 1-13 respectively. The substitutions were distributed in both VP5*andVP8* subunits ofVP4gene respectively of permeabilization and hemagglutinating activity. The presence of unique amino acid substitutions was identified in two of the four wild type (V377G, S387N in 061060and I644Lin0715880) and all four cell culture adapted (A46Vin0613158-CA, T60R in06361-CA, L237V, G389V andQ480H in061060-CA andS615G andT625Pin0715880-CA) strains for the first time in theVP4 gene ofP[8]specificity. Amino acid substitutions generated increase in the hydrophilicity in the cell culture adapted rotavirus strains as compared to their corresponding wild type strains.Conclusions: Amino acid substitutions detected in the VP4 genes ofG1P[8]rotavirus strains from this study together with those from other studies highlight occurrence of only strain and/or host specific substitutions during cell culture adaptation. Further evaluation of such substitutions for their role in attenuation, immunogenicity and conformation is needed for the development of newer rotavirus vaccines.

  18. G-1 exerts neuroprotective effects through G protein-coupled estrogen receptor 1 following spinal cord injury in mice

    Science.gov (United States)

    Cheng, Qiang; Meng, Jia; Wang, Xin-shang; Kang, Wen-bo; Tian, Zhen; Zhang, Kun; Liu, Gang; Zhao, Jian-ning

    2016-01-01

    Spinal cord injury (SCI) always occurs accidently and leads to motor dysfunction because of biochemical and pathological events. Estrogen has been shown to be neuroprotective against SCI through estrogen receptors (ERs), but the underlying mechanisms have not been fully elucidated. In the present study, we investigated the role of a newly found membrane ER, G protein-coupled estrogen receptor 1 (GPR30 or GPER1), and discussed the feasibility of a GPR30 agonist as an estrogen replacement. Forty adult female C57BL/6J mice (10–12 weeks old) were divided randomly into vehicle, G-1, E2, G-1 + G-15 and E2 + G-15 groups. All mice were subjected to SCI using a crushing injury approach. The specific GPR30 agonist, G-1, mimicked the effects of E2 treatment by preventing SCI-induced apoptotic cell death and enhancing motor functional recovery after injury. GPR30 activation regulated phosphatidylinositol 3-kinase (PI3K)/Akt and MAPK/extracellular signal-regulated kinase (ERK) signalling pathways, increased GPR30 and anti-apoptosis proteins Bcl-2 and brain derived neurotrophic factor (BDNF), but decreased the pro-apoptosis factor Bax and cleaved caspase-3. However, the neuroprotective effects of G-1 and E2 were blocked by the specific GPR30 antagonist, G-15. Thus, GPR30 rather than classic ERs is required to induce estrogenic neuroprotective effects. Given that estrogen replacement therapy may cause unexpected side effects, especially on the reproductive system, GPR30 agonists may represent a potential therapeutic approach for treating SCI. PMID:27407175

  19. Echinococcus ortleppi (G5) and Echinococcus granulosus sensu stricto (G1) loads in cattle from Southern Brazil.

    Science.gov (United States)

    Balbinotti, Helier; Santos, Guilherme B; Badaraco, Jeferson; Arend, Ana C; Graichen, Daniel Ângelo S; Haag, Karen L; Zaha, Arnaldo

    2012-09-10

    Echinococcus granulosus sensu stricto (G1) and Echinococcus ortleppi (G5) are haplotypes of the parasite formerly known as Echinococcus granulosus sensu lato, which in its larval stage causes cystic hydatid disease, endemic in Southern Brazil. Epidemiological and molecular knowledge about the haplotypes occurring in a region is essential to control the spread of the disease. The aim of this work was to analyze the haplotype frequency and fertility of hydatid cysts in cattle from the state of Rio Grande do Sul. Cysts were collected and classified according to their fertility status. DNA was extracted from protoscoleces and germinal layers and then used as template for the amplification of the cytochrome c oxidase subunit 1 gene by PCR. Amplicons were purified and sequenced, and the sequences were analyzed for haplotype identification. A total of 638 fertile cysts collected in the last ten years were genotyped. On average, G1 (56.6%) was more frequent than G5 (43.4%). In lungs, the G5 haplotype exhibited a higher parasite load (52.8%), whereas in the liver, G1 was more frequent (90.4%). The analysis revealed an increase in the frequency of G5 haplotype cysts during the period of sampling, and an increase in the abundance of fertile cysts has also been observed in the last several years. Most infertile cysts were genotyped as G1. The possible factors involved in the increase in the proportion of E. ortleppi (G5) and the consequences of this increase are discussed. This study suggests that the proportion of E. ortleppi (G5) loads in cattle may be increasing overtime.

  20. MKP1 phosphatase mediates G1-specific dephosphorylation of H3Serine10P in response to DNA damage

    International Nuclear Information System (INIS)

    Highlights: • Reversible reduction of H3S10 phosphorylation after DNA damage is G1 phase specific. • Dynamic balance between MAP kinases, MKP1 and MSK1 regulate H3S10P during DDR. • MKP1 associates with chromatin bearing γH2AX in response to DNA damage. • Inhibition of MKP1 activity with specific inhibitor promotes radiation-induced cell death. - Abstract: Histone mark, H3S10 phosphorylation plays a dual role in a cell by maintaining relaxed chromatin for active transcription in interphase and condensed chromatin state in mitosis. The level of H3S10P has also been shown to alter on DNA damage; however, its cell cycle specific behavior and regulation during DNA damage response is largely unexplored. In the present study, we demonstrate G1 cell cycle phase specific reversible loss of H3S10P in response to IR-induced DNA damage is mediated by opposing activities of phosphatase, MKP1 and kinase, MSK1 of the MAP kinase pathway. We also show that the MKP1 recruits to the chromatin in response to DNA damage and correlates with the decrease of H3S10P, whereas MKP1 is released from chromatin during recovery phase of DDR. Furthermore, blocking of H3S10 dephosphorylation by MKP1 inhibition impairs DNA repair process and results in poor survival of WRL68 cells. Collectively, our data proposes a pathway regulating G1 cell cycle phase specific reversible reduction of H3S10P on IR induced DNA damage and also raises the possibility of combinatorial modulation of H3S10P with specific inhibitors to target the cancer cells in G1-phase of cell cycle

  1. The hard X-ray view of the young supernova remnant G1.9+0.3

    DEFF Research Database (Denmark)

    Zoglauer, Andreas; Reynolds, Stephen P.; An, Hongjun;

    2015-01-01

    NuSTAR observed G1.9+0.3, the youngest known supernova remnant in the Milky Way, for 350 ks and detected emission up to ~30 keV. The remnant's X-ray morphology does not change significantly across the energy range from 3 to 20 keV. A combined fit between NuSTAR and Chandra shows that the spectrum...

  2. MKP1 phosphatase mediates G1-specific dephosphorylation of H3Serine10P in response to DNA damage

    Energy Technology Data Exchange (ETDEWEB)

    Sharma, Ajit K.; Khan, Shafqat A.; Sharda, Asmita; Reddy, Divya V; Gupta, Sanjay, E-mail: sgupta@actrec.gov.in

    2015-08-15

    Highlights: • Reversible reduction of H3S10 phosphorylation after DNA damage is G1 phase specific. • Dynamic balance between MAP kinases, MKP1 and MSK1 regulate H3S10P during DDR. • MKP1 associates with chromatin bearing γH2AX in response to DNA damage. • Inhibition of MKP1 activity with specific inhibitor promotes radiation-induced cell death. - Abstract: Histone mark, H3S10 phosphorylation plays a dual role in a cell by maintaining relaxed chromatin for active transcription in interphase and condensed chromatin state in mitosis. The level of H3S10P has also been shown to alter on DNA damage; however, its cell cycle specific behavior and regulation during DNA damage response is largely unexplored. In the present study, we demonstrate G1 cell cycle phase specific reversible loss of H3S10P in response to IR-induced DNA damage is mediated by opposing activities of phosphatase, MKP1 and kinase, MSK1 of the MAP kinase pathway. We also show that the MKP1 recruits to the chromatin in response to DNA damage and correlates with the decrease of H3S10P, whereas MKP1 is released from chromatin during recovery phase of DDR. Furthermore, blocking of H3S10 dephosphorylation by MKP1 inhibition impairs DNA repair process and results in poor survival of WRL68 cells. Collectively, our data proposes a pathway regulating G1 cell cycle phase specific reversible reduction of H3S10P on IR induced DNA damage and also raises the possibility of combinatorial modulation of H3S10P with specific inhibitors to target the cancer cells in G1-phase of cell cycle.

  3. Detailed petrographic descriptions and microprobe data for tertiary silicic volcanic rocks in drill hole USW G-1, Yucca Mountain, Nevada

    International Nuclear Information System (INIS)

    This report contains detailed petrographic descriptions of 74 thin sections from drill hole USW G-1 at Yucca Mountain, Nevada. These descriptions are keyed to the distinctions between devitrified, vitrophyre, vitric, and zeolitized intervals below the Topopah Spring Member repository horizon. The petrographic features of the zeolitized intervals down through the Crater Flat tuff, as well as the sorption properties determined from these intervals, suggest that these zeolite occurrences may each have comparable sorptive capability

  4. SiBCN-CNT/Graphene Paper Electrode

    Science.gov (United States)

    David, Lamuel; Singh, Gurpreet

    2014-03-01

    We demonstrate synthesis and electrochemical performance of novel molecular precursor-derived ceramic (PDC)/carbon nanotube embedded graphene self-supporting composite papers as Li-ion battery electrode. The papers were prepared through vacuum filtration of various PDC-graphene oxide (GO) dispersions in DI water followed by thermal reduction at elevated temperatures that resulted in a homogenous PDC/reduced GO papers that were highly crumpled, mechanically robust and consisted of a 3-D electrically conducting network. These electrodes showed electrochemical capacities as much as approx. 300 mAh.g-1 with respect to total weight of the electrode (approx. 500 mAh.g-1 w.r.t. active material), with negligible capacity loss for more than 1000 cycles. Boron-doped silicon carbon nitride (Si(B)CN/graphene) outperformed its un-doped counterparts (SiCN/graphene), both in terms of electrochemical capacity, cycling stability and coulombic efficiency.

  5. G1/S-regulated E2F-containing protein complexes bind to the mouse thymidine kinase gene promoter

    DEFF Research Database (Denmark)

    Dou, Q P; Zhao, S; Levin, A H;

    1994-01-01

    By performing DNase I footprint analysis, we had identified three distinct protein binding sequences (MT1, MT2, and MT3) located on the mouse thymidine kinase (TK) upstream promoter (Dou, Q.-P., Fridovich-Keil, J. L., and Pardee, A.B. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 1157-1161). Here we...... report that MT2 includes an E2F-like binding site (GTTCGCGGGCAAA), as shown by the following evidence. (i) MT2 bound specifically to an affinity-purified fusion human E2F protein. (ii) Both MT2 and an authentic E2F site (TTTCGCGCGCTTT) bound specifically to similar or identical nuclear protein complexes....... (iii) Formation of both these DNA-protein complexes were cell cycle-dependent: a G0/G1 phase-specific complex (E2F.G0/G1) was replaced by an S phase-specific complex(es) (E2F.S), whereas "free" E2F increased after the G1/S transition. (iv) Pulse inhibition of protein synthesis with cycloheximide...

  6. Asteroid phase curve analysis with the H, G 1, G 2 photometric phase function: application to the PTF survey observations

    Science.gov (United States)

    Penttilä, Antti; Cellino, Alberto; Lu, Xiaoping; Shevchenko, Vasilij G.; Muinonen, Karri

    2016-10-01

    Estimation of an asteroid's absolute magnitude H from its photometry is extremely important. The absolute magnitude relates the brightness of the asteroid to its size, if the geometric albedo is known. The shape of the phase curve can serve as a proxy for the taxonomic type of the asteroid in cases with no spectral information available [1,2].In 2012, the IAU adopted the H,G1,G2 function to replace the H,G function for phase curve analysis [3]. This new function improves the backscattering behavior of the curve with high- and low-albedo asteroids. The phase function (PF) can be applied to asteroids with multiple high-quality observations. If the number of observations is small, or their accuracy is low, problems may arise. The most apparent problem is that the parameter G or the parameters G1, G2 might be poorly estimated. The solution has been to fix to value of G or values of G1, G2 and estimate only the H. In our recent work [4], we offer a solution that can improve the current situation with the photometric fits with a small number of low-accuracy observations. We present a constrained nonlinear least-squares method for fitting the H,G1,G2 function that can improve the possible bias with low-accuracy data. Then, we revisit the two-parameter PF with new data and offer a new version, the H,G12* PF. Finally, we assess the problem with fixed G or G1, G2 parameters by introducing one-parameter models that relate to five taxonomic asteroid groups. We tie all the models together with three or two parameters, or a single parameter, with a statistical model selection procedure to select the best version for a particular data set.We have developed practical tools for the abovementioned algorithms. We apply the tools to a dataset of 8,900 asteroids with almost 500,000 photometric observations from the Palomar Transient Factory survey [5]. We report the effect of the revised H estimates on the geometric albedos in cases where WISE-mission size estimates are available.[1] D

  7. Fatigue risk fuzzy evaluation for high-risk operations based on G1 method%基于 G1法的高危作业疲劳风险模糊综合评判

    Institute of Scientific and Technical Information of China (English)

    陈述; 余迪; 吴黎明

    2014-01-01

    Fatigue is the direct or indirect cause of many accidents and is a serious threat to work safety .For fa-tigue risk evaluation of high-risk operations , the causes of operations fatigue were considered and a fuzzy compre-hensive evaluation method was proposed according to comprehensive application of G 1.First of all, a systematical analysis of the influencing factor was conducted based on the performance of fatigue , and an evaluation index sys-tem was constructed with criterion of management , job, environment, physiological and psychological factors . Then, the weights of indexes were determined by applying the method of G 1 and the multilevel fuzzy comprehensive evaluation model of high-risk operations fatigue risk was built to judge fatigue .Finally , the feasibility and effective-ness of the method were illustrated by an example .It provides a new perspective and tool for the comprehensive e-valuation on the likelihood of high-risk operations fatigue .%疲劳严重威胁作业安全,许多事故都直接或间接由疲劳引发。针对高危作业疲劳风险评价问题,考虑作业疲劳致因,综合运用G1法,提出高危作业疲劳风险的模糊综合评判方法。首先,根据疲劳的表现,系统分析影响高危作业疲劳的因素,以管理因素、岗位因素、作业环境、生理、心理等5个因素为准则,构建作业疲劳风险的评价指标体系。然后,应用G1法,确定指标权重,建立高危作业疲劳风险多级模糊综合评价模型,评判疲劳状况。最后,通过一个案例验证了模型方法的可行性与有效性,为综合评判高危作业疲劳发生可能性提供了一个新视角与方法。

  8. 24 CFR Appendix Ms-2 to Part 3500 - Appendix MS-2 to Part 3500

    Science.gov (United States)

    2010-04-01

    ... HOUSING AND URBAN DEVELOPMENT REAL ESTATE SETTLEMENT PROCEDURES ACT Pt. 3500, App. MS-2 Appendix MS-2 to... 24 Housing and Urban Development 5 2010-04-01 2010-04-01 false Appendix MS-2 to Part 3500 MS Appendix MS-2 to Part 3500 Housing and Urban Development Regulations Relating to Housing and...

  9. 24 CFR Appendix Ms-1 to Part 3500 - Appendix MS-1 to Part 3500

    Science.gov (United States)

    2010-04-01

    ... HOUSING AND URBAN DEVELOPMENT REAL ESTATE SETTLEMENT PROCEDURES ACT Pt. 3500, App. MS-1 Appendix MS-1 to... 24 Housing and Urban Development 5 2010-04-01 2010-04-01 false Appendix MS-1 to Part 3500 MS Appendix MS-1 to Part 3500 Housing and Urban Development Regulations Relating to Housing and...

  10. 13 CFR Appendix A to Subpart A of... - Appendix A to Subpart A of Part 113

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Appendix A to Subpart A of Part 113 A Appendix A to Subpart A of Part 113 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION... ADMINISTRATOR General Provisions Pt. 113, Subpart A, App. A Appendix A to Subpart A of Part 113 Name of...

  11. 43 CFR Appendix A to Subpart A of... - Appendix A to Subpart A of Part 17

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Appendix A to Subpart A of Part 17 A Appendix A to Subpart A of Part 17 Public Lands: Interior Office of the Secretary of the Interior... Race, Color, or National Origin Pt. 17, Subpt. A, App. A Appendix A to Subpart A of Part 17...

  12. Analysis of an M/G/1 Queue with Multiple Vacations, N-policy, Unreliable Service Station and Repair Facility Failures

    Directory of Open Access Journals (Sweden)

    Wenqing Wu

    2014-05-01

    Full Text Available This paper studies an M/G/1 repairable queueing system with multiple vacations and N-policy, in which the service station is subject to occasional random breakdowns. When the service station breaks down, it is repaired by a repair facility. Moreover, the repair facility may fail during the repair period of the service station. The failed repair facility resumes repair after completion of its replacement. Under these assumptions, applying a simple method, the probability that the service station is broken, the rate of occurrence of breakdowns of the service station, the probability that the repair facility is being replaced and the rate of occurrence of failures of the repair facility along with other performance measures are obtained. Following the construction of the long-run expected cost function per unit time, the direct search method is implemented for determining the optimum threshold N* that minimises the cost function.

  13. The Departure Process for the Mx/G/1 Queueing System with Server Vacations%多重休假Mx/G/1排队系统的输出过程

    Institute of Scientific and Technical Information of China (English)

    唐应辉

    2007-01-01

    We firstly study the departure process of Mx/G/1 queueing system with multiple server vacations.By using direct probability decomposition,renewal theory and the Laplace-Stieltjes transform,we discuss the expected number of departures occurring in the time interval (0,t] from the beginning of the any initial state i(i=0,1,2,…) and its asymptotic expansion.%本文首次研究服务员具有多重休假规则的成批到达Mx/G/1排队系统的输出过程.应用更新过程理论、拉普拉斯-司梯阶变换和本文提出的直接概率分解分析法,讨论了从任意初始状态出发,系统在(0,t]时间内输出顾客的平均数,以及其渐近展开,得到一些重要结果.

  14. Impacts, effectiveness and regional inequalities of the GeoMIP G1 to G4 solar radiation management scenarios

    Science.gov (United States)

    Yu, Xiaoyong; Moore, John C.; Cui, Xuefeng; Rinke, Annette; Ji, Duoying; Kravitz, Ben; Yoon, Jin-Ho

    2015-06-01

    We evaluate the effectiveness and the regional inequalities of solar radiation management (SRM) in compensating for simultaneous changes in temperature and precipitation caused by increased greenhouse gas concentrations. We analyze the results from Earth System Models under four Geoengineering Model Intercomparison Project (GeoMIP) experiments with a modified form of the Residual Climate Response approach. Each experiment produces 50 model yrs of simulations: 13 models completed experiment G1 (offsetting 4 × CO2 via solar reduction); 12 models completed experiment G2 (offsetting CO2 that increased by 1% per year); 3 models completed experiment G3 (offsetting increasing radiative forcing under RCP4.5 with increasing stratospheric aerosol); and 7 models completed experiment G4 (injection of 5 Tg SO2 a- 1 into the stratosphere). The regional inequalities in temperature and precipitation compensation for experiments G1, G3 and G4 are significantly different from their corresponding noise backgrounds for most models, but for G2 they are not significantly different from noise. Differences in the regional inequalities and the actual effectiveness among the four SRM scenarios are not significant for many models. However, in more than half of the models, the effectiveness for temperature in the solar dimming geoengineering scenarios (G1 and G2) is significantly higher than that in the SO2 geoengineering scenarios (G3 and G4). The effectiveness of the four SRM experiments in compensating for temperature change is considerably higher than for precipitation. The methodology used highlights that a large across-model variation in the treatment of key geoengineering processes (such as stratospheric aerosols) and the quantification of damage caused by climate change creates significant uncertainties in any strategies to achieve optimal compensation effectiveness across different regions.

  15. G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells

    Directory of Open Access Journals (Sweden)

    Chen Jiapei

    2005-08-01

    Full Text Available Abstract Severe Acute Respiratory Syndrome coronavirus (SARS-CoV, cause of the life-threatening atypical pneumonia, infects many organs, such as lung, liver and immune organ, and induces parenchyma cells apoptosis and necrosis. The genome of SARS-CoV, not closely related to any of the previously characterized coronavirus, encodes replicase and four major structural proteins and a number of non-structural proteins. Published studies suggest that some non-structural proteins may play important roles in the replication, virulence and pathogenesis of viruses. Among the potential SARS-CoV non-structural proteins, 3b protein (ORF4 is predicted encoding 154 amino acids, lacking significant similarities to any known proteins. Till now, there is no report about the function of 3b protein. In this study, 3b gene was linked with the EGFP tag at the C- terminus. Through cell cycle analysis, it was found that over-expression of 3b-EGFP protein in Vero, 293 and COS-7 cells could induce cell cycle arrest at G0/G1 phase, and that especially in COS-7 cells, expression of 3b-EGFP was able to induce the increase of sub-G1 phase from 24 h after transfection, which was most obvious at 48 h. The apoptosis induction of 3b fusion protein in COS-7 cells was further confirmed by double cell labeling with 7-AAD and Annexin V, the function of 3b protein inducing cell G0/G1 arrest and apoptosis may provide a new insight for further study on the mechanism of SARS pathogenesis.

  16. Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe

    DEFF Research Database (Denmark)

    Davey, William John; Nielsen, O; Nielsen, Olaf

    1994-01-01

    a transient G1 arrest of cell division, transcription of mat1-Pm, and elongation of the cells to form shmoos. The second mutant contains the temperature-sensitive pat1-114 allele. At 30 degrees C this mutant was previously shown not only to bypass the nutritional signal but also to stop growing in a state...... derepressed for pheromone-controlled functions. We now report that an h+ pat1-114 strain growing mitotically at 23 degrees C responds to M-factor. This shows that the pat1 protein kinase can be tuned to derepress nutritional signalling while repressing the other stages in the differentiation process....

  17. Molecular interplay between cdk4 and p21 dictates G0/G1 cell cycle arrest in prostate cancer cells

    OpenAIRE

    Gulappa, Thippeswamy; Reddy, Ramadevi Subramani; Suman, Suman; Nyakeriga, Alice M; Damodaran, Chendil

    2013-01-01

    This study examined the effect of 3, 9-dihydroxy-2-prenylcoumestan (pso), a furanocoumarin, on PC-3 and C4-2B castration-resistant prostate cancer (CRPC) cell lines. Pso caused significant G0/G1 cell cycle arrest and inhibition of cell growth. Molecular analysis of cyclin (D1, D2, D3, and E), cyclin-dependent kinase (cdk) (cdks 2, 4, and 6), and cdk inhibitor (p21 and p27) expression suggested transcriptional regulation of the cdk inhibitors and more significant downregulation of cdk4 than of...

  18. Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution

    OpenAIRE

    Traxlmayr, Michael W.; Lobner, Elisabeth; Hasenhindl, Christoph; Stadlmayr, Gerhard; Oostenbrink, Chris; Rüker, Florian; Obinger, Christian

    2014-01-01

    For most therapeutic proteins, a long serum half-life is desired. Studies have shown that decreased antigen binding at acidic pH can increase serum half-life. In this study, we aimed to investigate whether pH-dependent binding sites can be introduced into antigen binding crystallizable fragments of immunoglobulin G1 (Fcab). The C-terminal structural loops of an Fcab were engineered for reduced binding to the extracellular domain of human epidermal growth factor receptor 2 (Her2-ECD) at pH 6 c...

  19. On the Discrete-Time GeoX/G/1 Queues under N-Policy with Single and Multiple Vacations

    Directory of Open Access Journals (Sweden)

    Sung J. Kim

    2013-01-01

    Full Text Available We consider the discrete-time GeoX/G/1 queue under N-policy with single and multiple vacations. In this queueing system, the server takes multiple vacations and a single vacation whenever the system becomes empty and begins to serve customers only if the queue length is at least a predetermined threshold value N. Using the well-known property of stochastic decomposition, we derive the stationary queue-length distributions for both vacation models in a simple and unified manner. In addition, we derive their busy as well as idle-period distributions. Some classical vacation models are considered as special cases.

  20. ANALYSIS AND COMPUTATIONAL ALGORITHM FOR QUEUES WITH STATE-DEPENDENT VACATIONS Ⅱ: M(n)/G/1/K

    Institute of Scientific and Technical Information of China (English)

    Xiuli CHAO; Ayyar RAHMAN

    2006-01-01

    We study a single-server queueing system with state-dependent arrivals and general service distribution, or simply M(n)/G/1/K, where the server follows an N policy and takes multiple vacations when the system is empty. We provide a recursive algorithm using the supplementary variable technique to numerically compute the stationary queue length distribution of the system. The only input requirements are the Laplace-Stieltjes transforms of the service time distribution and the vacation time distribution, and the state-dependent arrival rate. The computational complexity of the algorithm is O(K3).

  1. Approximation of the steady state system state distribution of the M/G/1 retrial queue with impatient customers

    Directory of Open Access Journals (Sweden)

    Stihi Nadjet

    2012-01-01

    Full Text Available For M/G/1 retrial queues with impatient customers, we review the results, concerning the steady state distribution of the system state, presented in the literature. Since the existing formulas are cumbersome (so their utilization in practice becomes delicate or the obtaining of these formulas is impossible, we apply the information theoretic techniques for estimating the above mentioned distribution. More concretely, we use the principle of maximum entropy which provides an adequate methodology for computing a unique estimate for an unknown probability distribution based on information expressed in terms of some given mean value constraints.

  2. Molecular basis for vulnerability to mitochondrial and oxidative stress in a neuroendocrine CRI-G1 cell line.

    Directory of Open Access Journals (Sweden)

    Natasha Chandiramani

    Full Text Available BACKGROUND: Many age-associated disorders (including diabetes, cancer, and neurodegenerative diseases are linked to mitochondrial dysfunction, which leads to impaired cellular bioenergetics and increased oxidative stress. However, it is not known what genetic and molecular pathways underlie differential vulnerability to mitochondrial dysfunction observed among different cell types. METHODOLOGY/PRINCIPAL FINDINGS: Starting with an insulinoma cell line as a model for a neuronal/endocrine cell type, we isolated a novel subclonal line (named CRI-G1-RS that was more susceptible to cell death induced by mitochondrial respiratory chain inhibitors than the parental CRI-G1 line (renamed CRI-G1-RR for clarity. Compared to parental RR cells, RS cells were also more vulnerable to direct oxidative stress, but equally vulnerable to mitochondrial uncoupling and less vulnerable to protein kinase inhibition-induced apoptosis. Thus, differential vulnerability to mitochondrial toxins between these two cell types likely reflects differences in their ability to handle metabolically generated reactive oxygen species rather than differences in ATP production/utilization or in downstream apoptotic machinery. Genome-wide gene expression analysis and follow-up biochemical studies revealed that, in this experimental system, increased vulnerability to mitochondrial and oxidative stress was associated with (1 inhibition of ARE/Nrf2/Keap1 antioxidant pathway; (2 decreased expression of antioxidant and phase I/II conjugation enzymes, most of which are Nrf2 transcriptional targets; (3 increased expression of molecular chaperones, many of which are also considered Nrf2 transcriptional targets; (4 increased expression of β cell-specific genes and transcription factors that specify/maintain β cell fate; and (5 reconstitution of glucose-stimulated insulin secretion. CONCLUSIONS/SIGNIFICANCE: The molecular profile presented here will enable identification of individual genes or

  3. Some Reliability Problems Arising in the Mx/G(M/G)/1 Repairable Queueing System with Single Delay Vacation

    Institute of Scientific and Technical Information of China (English)

    TANG Ying-hui

    2001-01-01

    On the basis of Ref. [1], the Mx/G(M/G)/1 repairable queueing system with single delay vacation is discussed again. The following reliability problems of the service station are studied: (a) The probability that it fails at time t, i.e. its unavailability; (b) The expected failure number during the time interval (0,t]; (c) The expected failure number during the "server busy period"; (d) The expected downtime during the time interval (0,t]. Some reliability results of the service station are obtained. These results would be interest to reliability analysts.

  4. G0/G1 switch gene-2 regulates human adipocyte lipolysis by affecting activity and localization of adipose triglyceride lipase

    OpenAIRE

    Schweiger, Martina; Paar, Margret; Eder, Christina; Brandis, Janina; Moser, Elena; Gorkiewicz, Gregor; Grond, Susanne; Radner, Franz P. W.; Cerk, Ines; Cornaciu, Irina; Oberer, Monika; Kersten, Sander; Zechner, Rudolf; Zimmermann, Robert; Lass, Achim

    2012-01-01

    The hydrolysis of triglycerides in adipocytes, termed lipolysis, provides free fatty acids as energy fuel. Murine lipolysis largely depends on the activity of adipose triglyceride lipase (ATGL), which is regulated by two proteins annotated as comparative gene identification-58 (CGI-58) and G0/G1 switch gene-2 (G0S2). CGI-58 activates and G0S2 inhibits ATGL activity. In contrast to mice, the functional role of G0S2 in human adipocyte lipolysis is poorly characterized. Here we show that overexp...

  5. Stanford Occasional Papers in Linguistics, No. 3. Papers from the Annual California Linguistics Conference (3rd, May 5-6, 1973).

    Science.gov (United States)

    Gee, James Paul, Ed.; And Others

    This volume includes 12 of the 24 papers presented at the Third Annual California Linguistics Conference. Selections are drawn from each of the four sessions, covering semantic and lexical structure, phonology, syntax, and language in context. Each of the papers includes a bibliography, as well as diagrams, charts, and appendixes when necessary.…

  6. TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells.

    Science.gov (United States)

    Pedersen, Rune Troelsgaard; Kruse, Thomas; Nilsson, Jakob; Oestergaard, Vibe H; Lisby, Michael

    2015-08-17

    Genome integrity is critically dependent on timely DNA replication and accurate chromosome segregation. Replication stress delays replication into G2/M, which in turn impairs proper chromosome segregation and inflicts DNA damage on the daughter cells. Here we show that TopBP1 forms foci upon mitotic entry. In early mitosis, TopBP1 marks sites of and promotes unscheduled DNA synthesis. Moreover, TopBP1 is required for focus formation of the structure-selective nuclease and scaffold protein SLX4 in mitosis. Persistent TopBP1 foci transition into 53BP1 nuclear bodies (NBs) in G1 and precise temporal depletion of TopBP1 just before mitotic entry induced formation of 53BP1 NBs in the next cell cycle, showing that TopBP1 acts to reduce transmission of DNA damage to G1 daughter cells. Based on these results, we propose that TopBP1 maintains genome integrity in mitosis by controlling chromatin recruitment of SLX4 and by facilitating unscheduled DNA synthesis.

  7. An APC/C-Cdh1 Biosensor Reveals the Dynamics of Cdh1 Inactivation at the G1/S Transition.

    Science.gov (United States)

    Ondracka, Andrej; Robbins, Jonathan A; Cross, Frederick R

    2016-01-01

    B-type cyclin-dependent kinase activity must be turned off for mitotic exit and G1 stabilization. B-type cyclin degradation is mediated by the anaphase-promoting complex/cyclosome (APC/C); during and after mitotic exit, APC/C is dependent on Cdh1. Cdh1 is in turn phosphorylated and inactivated by cyclin-CDK at the Start transition of the new cell cycle. We developed a biosensor to assess the cell cycle dynamics of APC/C-Cdh1. Nuclear exit of the G1 transcriptional repressor Whi5 is a known marker of Start; APC/C-Cdh1 is inactivated 12 min after Whi5 nuclear exit with little measurable cell-to-cell timing variability. Multiple phosphorylation sites on Cdh1 act in a redundant manner to repress its activity. Reducing the number of phosphorylation sites on Cdh1 can to some extent be tolerated for cell viability, but it increases variability in timing of APC/C-Cdh1 inactivation. Mutants with minimal subsets of phosphorylation sites required for viability exhibit striking stochasticity in multiple responses including budding, nuclear division, and APC/C-Cdh1 activity itself. Multiple cyclin-CDK complexes, as well as the stoichiometric inhibitor Acm1, contribute to APC/C-Cdh1 inactivation; this redundant control is likely to promote rapid and reliable APC/C-Cdh1 inactivation immediately following the Start transition.

  8. Effects of furanodiene on 95-D lung cancer cells: apoptosis, autophagy and G1 phase cell cycle arrest.

    Science.gov (United States)

    Xu, Wen-Shan; Li, Ting; Wu, Guo-Sheng; Dang, Yuan-Ye; Hao, Wen-Hui; Chen, Xiu-Ping; Lu, Jin-Jian; Wang, Yi-Tao

    2014-01-01

    Furanodiene (FUR) is a natural terpenoid isolated from Rhizoma curcumae, a well-known Chinese medicinal herb that presents anti-proliferative activities in several cancer cell lines. Herein, we systematically investigated the effects of FUR on the significant processes of tumor progression with the relatively low concentrations in 95-D lung cancer cells. FUR concentration-dependently inhibited cell proliferation and blocked the cell cycle progressions in G1 phase by down-regulating the protein levels of cyclin D1 and CDK6, and up-regulating those of p21 and p27 in 95-D cells. FUR also affected the signaling molecules that regulate apoptosis in 95-D cells revealed by the down-regulation of the protein levels of full PARP, pro-caspase-7, survivin, and Bcl-2, and the up-regulation of cleaved PARP. Further studies showed that FUR enhanced the expression of light chain 3-II (LC3-II) in the protein level, indicating that autophagy is involved in this process. Besides, the adhesion ability of 95-D cells to matrigel and fibronectin was slightly inhibited after FUR treatment for 1 h in our experimental condition. FUR also slightly suppressed cell migration and invasion in 95-D cells according to the data from wound healing and Transwell assays, respectively. Taken together, FUR activated the signal molecules regulating G1 cell cycle arrest, apoptosis and autophagy, while slightly affecting the key steps of cell metastasis in 95-D lung cancer cells in the relatively low concentrations.

  9. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico.

    Science.gov (United States)

    Villa-Mancera, Abel; Molina-Mendoza, Pedro; Hernández-Guzmán, Karina; Olivares-Pérez, Jaime; Sarracent-Pérez, Jorge; Zumaquero-Ríos, José

    2016-01-01

    The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P < 0.01) for low seropositivity (r = 0.93) and medium seropositivity (r = 0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans. PMID:27563665

  10. Betulinic Acid Inhibits Growth of Cultured Vascular Smooth Muscle Cells In Vitro by Inducing G1 Arrest and Apoptosis

    Directory of Open Access Journals (Sweden)

    Raja Kumar Vadivelu

    2012-01-01

    Full Text Available Betulinic acid is a widely available plant-derived triterpene which is reported to possess selective cytotoxic activity against cancer cells of neuroectodermal origin and leukemia. However, the potential of betulinic acid as an antiproliferative and cytotoxic agent on vascular smooth muscle (VSMC is still unclear. This study was carried out to demonstrate the antiproliferative and cytotoxic effect of betulinic acid on VSMCs using 3-[4,5-dimethylthizol-2-yl]-2,5-diphenyltetrazolium bromide (MTT assay, flow cytometry cell cycle assay, BrdU proliferation assay, acridine orange/propidium iodide staining, and comet assay. Result from MTT and BrdU assays indicated that betulinic acid was able to inhibit the growth and proliferation of VSMCs in a dose-dependent manner with IC50 of 3.8 μg/mL significantly (P<0.05. Nevertheless, betulinic acid exhibited G1 cell cycle arrest in flow cytometry cell cycle profiling and low level of DNA damage against VSMC in acridine orange/propidium iodide and comet assay after 24 h of treatment. In conclusion, betulinic acid induced G1 cell cycle arrest and dose-dependent DNA damage on VSMC.

  11. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico.

    Science.gov (United States)

    Villa-Mancera, Abel; Molina-Mendoza, Pedro; Hernández-Guzmán, Karina; Olivares-Pérez, Jaime; Sarracent-Pérez, Jorge; Zumaquero-Ríos, José

    2016-01-01

    The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P < 0.01) for low seropositivity (r = 0.93) and medium seropositivity (r = 0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans.

  12. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

    Directory of Open Access Journals (Sweden)

    Abel Villa-Mancera

    2016-01-01

    Full Text Available The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P<0.01 for low seropositivity (r=0.93 and medium seropositivity (r=0.84. The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans.

  13. TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells

    DEFF Research Database (Denmark)

    Pedersen, Rune Troelsgaard; Kruse, Thomas; Nilsson, Jakob;

    2015-01-01

    Genome integrity is critically dependent on timely DNA replication and accurate chromosome segregation. Replication stress delays replication into G2/M, which in turn impairs proper chromosome segregation and inflicts DNA damage on the daughter cells. Here we show that TopBP1 forms foci upon...... mitotic entry. In early mitosis, TopBP1 marks sites of and promotes unscheduled DNA synthesis. Moreover, TopBP1 is required for focus formation of the structure-selective nuclease and scaffold protein SLX4 in mitosis. Persistent TopBP1 foci transition into 53BP1 nuclear bodies (NBs) in G1 and precise...... temporal depletion of TopBP1 just before mitotic entry induced formation of 53BP1 NBs in the next cell cycle, showing that TopBP1 acts to reduce transmission of DNA damage to G1 daughter cells. Based on these results, we propose that TopBP1 maintains genome integrity in mitosis by controlling chromatin...

  14. An APC/C-Cdh1 Biosensor Reveals the Dynamics of Cdh1 Inactivation at the G1/S Transition

    Science.gov (United States)

    Ondracka, Andrej; Robbins, Jonathan A.; Cross, Frederick R.

    2016-01-01

    B-type cyclin-dependent kinase activity must be turned off for mitotic exit and G1 stabilization. B-type cyclin degradation is mediated by the anaphase-promoting complex/cyclosome (APC/C); during and after mitotic exit, APC/C is dependent on Cdh1. Cdh1 is in turn phosphorylated and inactivated by cyclin-CDK at the Start transition of the new cell cycle. We developed a biosensor to assess the cell cycle dynamics of APC/C-Cdh1. Nuclear exit of the G1 transcriptional repressor Whi5 is a known marker of Start; APC/C-Cdh1 is inactivated 12 min after Whi5 nuclear exit with little measurable cell-to-cell timing variability. Multiple phosphorylation sites on Cdh1 act in a redundant manner to repress its activity. Reducing the number of phosphorylation sites on Cdh1 can to some extent be tolerated for cell viability, but it increases variability in timing of APC/C-Cdh1 inactivation. Mutants with minimal subsets of phosphorylation sites required for viability exhibit striking stochasticity in multiple responses including budding, nuclear division, and APC/C-Cdh1 activity itself. Multiple cyclin-CDK complexes, as well as the stoichiometric inhibitor Acm1, contribute to APC/C-Cdh1 inactivation; this redundant control is likely to promote rapid and reliable APC/C-Cdh1 inactivation immediately following the Start transition. PMID:27410035

  15. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

    Science.gov (United States)

    Molina-Mendoza, Pedro; Hernández-Guzmán, Karina; Olivares-Pérez, Jaime; Sarracent-Pérez, Jorge; Zumaquero-Ríos, José

    2016-01-01

    The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P < 0.01) for low seropositivity (r = 0.93) and medium seropositivity (r = 0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans. PMID:27563665

  16. Human IgG1 Responses to Surface Localised Schistosoma mansoni Ly6 Family Members Drop following Praziquantel Treatment.

    Directory of Open Access Journals (Sweden)

    Iain W Chalmers

    Full Text Available The heptalaminate-covered, syncytial tegument is an important anatomical adaptation that enables schistosome parasites to maintain long-term, intravascular residence in definitive hosts. Investigation of the proteins present in this surface layer and the immune responses elicited by them during infection is crucial to our understanding of host/parasite interactions. Recent studies have revealed a number of novel tegumental surface proteins including three (SmCD59a, SmCD59b and Sm29 containing uPAR/Ly6 domains (renamed SmLy6A SmLy6B and SmLy6D in this study. While vaccination with SmLy6A (SmCD59a and SmLy6D (Sm29 induces protective immunity in experimental models, human immunoglobulin responses to representative SmLy6 family members have yet to be thoroughly explored.Using a PSI-BLAST-based search, we present a comprehensive reanalysis of the Schistosoma mansoni Ly6 family (SmLy6A-K. Our examination extends the number of members to eleven (including three novel proteins and provides strong evidence that the previously identified vaccine candidate Sm29 (renamed SmLy6D is a unique double uPAR/Ly6 domain-containing representative. Presence of canonical cysteine residues, signal peptides and GPI-anchor sites strongly suggest that all SmLy6 proteins are cell surface-bound. To provide evidence that SmLy6 members are immunogenic in human populations, we report IgG1 (as well as IgG4 and IgE responses against two surface-bound representatives (SmLy6A and SmLy6B within a cohort of S. mansoni-infected Ugandan males before and after praziquantel treatment. While pre-treatment IgG1 prevalence for SmLy6A and SmLy6B differs amongst the studied population (7.4% and 25.3% of the cohort, respectively, these values are both higher than IgG1 prevalence (2.7% for a sub-surface tegumental antigen, SmTAL1. Further, post-treatment IgG1 levels against surface-associated SmLy6A and SmLy6B significantly drop (p = 0.020 and p < 0.001, respectively when compared to rising IgG

  17. A Discrete-Time Geo/G/1 Retrial Queue with Bernoulli Vacation and Second Optional Service%有Bernoulli休假和可选服务的Geo/G/1重试排队

    Institute of Scientific and Technical Information of China (English)

    陈佩树; 朱翼隽; 陈燕

    2011-01-01

    讨论了有Bernoulli休假策略和可选服务的离散时间Geo/G/1重试排队系统.假定一旦顾客发现服务台忙或在休假就进入重试区域,重试时间服从几何分布.顾客在进行第一阶段服务结束后可以离开系统或进一步要求可选服务.服务台在每次服务完毕后,可以进行休假,或者等待服务下一个顾客.还研究了在此模型下的马尔可夫链,并计算了在稳态条件下的系统的各种性能指标以及给出一些特例和系统的随机分解.%We analyze a discrete-time Geo/G/1retrial queue with Bernoulli vacation where all the arriving customers require a first essential service while only some of them demand a second optional service. If upon arrival, the server is busy or vacation, the CU8tomer is obliged to leave the service area and to orbit. Each customer in the orbit forms an independent retrial source and the retrial time follows a geometrical law. Just after completion of a customer's service the server may take a vacation of random length or may opt to continue staying in the system to serve the next customer. We study the Markov chain underlying the considered queuing system and some performance measures of the system in steady-state. Further, we give two stochastic decomposition laws and some examples.

  18. Double logarithms, $ln^2(1/x)$, and the NLO DGLAP evolution for the non-singlet component of the nucleon spin structure function, $g_1$

    OpenAIRE

    Ziaja, Beata

    2002-01-01

    Theoretical predictions show that at low values of Bjorken $x$ the spin structure function, $g_1$ is influenced by large logarithmic corrections, $ln^2(1/x)$, which may be predominant in this region. These corrections are also partially contained in the NLO part of the standard DGLAP evolution. Here we calculate the non-singlet component of the nucleon structure function, $g_1^{NS}=g_1^p-g_1^n$, and its first moment, using a unified evolution equation. This equation incorporates the terms des...

  19. Acute appendicitis: position paper, WSES, 2013.

    Science.gov (United States)

    Agresta, Ferdinando; Ansaloni, Luca; Catena, Fausto; Verza, Luca Andrea; Prando, Daniela

    2014-01-01

    Appendectomy is one of the most frequently performed operative procedures in general surgery departments of every size and category. Laparoscopic Appendectomy - LA - as compared to Open Appendectomy - OA - was very controversial at first but has found increasing acceptance all over the World, although the percentage of its acceptance is different in the various single National setting. Various meta-analyses and Cochrane reviews have compared LA with OA and different technical details. Furthermore, new surgical methods have recently emerged, namely, the single-port/incision laparoscopic appendectomy and NOTES technique. Their distribution among the hospitals, however, is unclear. Using laparoscopic mini-instruments with trocars of 2-3.5 mm diameter is proposed as a reliable alternative due to less postoperative pain and improved aesthetics. How to proceed in case of an inconspicuous appendix during a procedure planned as an appendectomy remains controversial despite existing study results. But the main question still is: operate or not operate an acute appendicitis, in the meaning of an attempt of a conservative antibiotic therapy. Therefore, we have done a literature survey on the performance of appendectomies and their technical details as well as the management of the intraoperative finding of an inconspicuous appendix in order to write down - under the light of the latest evidence - a position paper. PMID:24708651

  20. Api5 contributes to E2F1 control of the G1/S cell cycle phase transition.

    Directory of Open Access Journals (Sweden)

    Marina Garcia-Jove Navarro

    Full Text Available BACKGROUND: The E2f transcription factor family has a pivotal role in controlling the cell fate in general, and in particular cancer development, by regulating the expression of several genes required for S phase entry and progression through the cell cycle. It has become clear that the transcriptional activation of at least one member of the family, E2F1, can also induce apoptosis. An appropriate balance of positive and negative regulators appears to be necessary to modulate E2F1 transcriptional activity, and thus cell fate. METHODOLOGY/PRINCIPAL FINDINGS: In this report, we show that Api5, already known as a regulator of E2F1 induced-apoptosis, is required for the E2F1 transcriptional activation of G1/S transition genes, and consequently, for cell cycle progression and cell proliferation. Api5 appears to be a cell cycle regulated protein. Removal of Api5 reduces cyclin E, cyclin A, cyclin D1 and Cdk2 levels, causing G1 cell cycle arrest and cell cycle delay. Luciferase assays established that Api5 directly regulates the expression of several G1/S genes under E2F1 control. Using protein/protein and protein/DNA immunoprecipitation studies, we demonstrate that Api5, even if not physically interacting with E2F1, contributes positively to E2F1 transcriptional activity by increasing E2F1 binding to its target promoters, through an indirect mechanism. CONCLUSION/SIGNIFICANCE: The results described here support the pivotal role of cell cycle related proteins, that like E2F1, may act as tumor suppressors or as proto-oncogenes during cancer development, depending on the behavior of their positive and negative regulators. According to our findings, Api5 contributes to E2F1 transcriptional activation of cell cycle-associated genes by facilitating E2F1 recruitment onto its target promoters and thus E2F1 target gene transcription.

  1. 31 CFR Appendixes to Chapter V - Note

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Note Appendixes to Chapter V Money... CONTROL, DEPARTMENT OF THE TREASURY Ch. V, Nt. Appendixes to Chapter V—Note Notes: The alphabetical lists.... References to regulatory parts in chapter V or other authorities: : Western Balkans Stabilization...

  2. Do comets C/1861 G1 (Thatcher) and C/1861 J1 (Great comet) have a common origin?

    Science.gov (United States)

    Branham, R. L.

    2015-10-01

    A new orbit is calculated for Comet C/1861 G1 (Thatcher), associated with the Lyrid meteor shower, to replace Oppolzer's orbit of 1864. The new orbit is based upon 649 observations, 326 in right ascension and 323 in declination, made between 11 April 1861 and 7 Sept. 1861. The final orbit uses residuals calculated with the Welsch weighting function. The comet's period of 416.87 ± 0.56 yr agrees with Oppolzer's period of 415 yr athough other elements such as the inclination differ. Although the post-perihelion residuals are relatively random, 52.1% probability of randomness, pre-perihelion residuals lack randomness indicating possible deviations from Keplerian motion caused by ejection of meteoritic material. Comet Thatcher is unrelated to the Great comet of 1861.

  3. Phylogenetic relationships among Brazilian howler monkeys, genus Alouatta (Platyrrhini, Atelidae, based on g1-globin pseudogene sequences

    Directory of Open Access Journals (Sweden)

    Carla Maria Meireles

    1999-09-01

    Full Text Available The genus Alouatta (howler monkeys is the most widely distributed of New World primates, and has been arranged in three species groups: the Central American Alouatta palliata group and the South American Alouatta seniculus and Alouatta caraya groups. While the latter is monotypic, the A. seniculus group encompasses at least three species (A. seniculus, A. belzebul and A. fusca. In the present study, approximately 600 base pairs of the g1-globin pseudogene were sequenced in the four Brazilian species (A. seniculus, A. belzebul, A. fusca and A. caraya. Maximum parsimony and maximum likelihood methods yielded phylogenetic trees with the same arrangement: {A. caraya [A. seniculus (A. fusca, A. belzebul]}. The most parsimonious tree had bootstrap values greater than 82% for all groupings, and strength of grouping values of at least 2, supporting the sister clade of A. fusca and A. belzebul. The study also confirmed the presence of a 150-base pair Alu insertion element and a 1.8-kb deletion in the g1-globin pseudogene in A. fusca, features found previously in the remaining three species. The cladistic classification based on molecular data agrees with those of morphological studies, with the monospecific A. caraya group being clearly differentiated from the A. seniculus group.Os guaribas, do gênero Alouatta, que são os primatas do Novo Mundo com maior distribuição geográfica, têm sido colocados em três grupos de espécies: o grupo Alouatta palliata da América central, e os grupos sulamericanos Alouatta seniculus e Alouatta caraya. Este último é monotípico, mas o grupo A. seniculus inclui pelo menos três espécies (A. seniculus, A. belzebul e A. fusca. Neste estudo, foram seqüenciados aproximadamente 600 pares de base do pseudogene globina g1 nas quatro espécies brasileiras (A. seniculus, A. belzebul, A. fusca e A. caraya. Os métodos de máxima parcimônia e máxima verossimilhança produziram árvores filogenéticas com o mesmo arranjo

  4. mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C.elegans

    Institute of Scientific and Technical Information of China (English)

    Min Liu; Pengpeng Liu; Li Zhang; Qingchun Cai; Ge Gao; Wenxia Zhang; Dong Liu; Qichang Fan; Zuoyan Zhu

    2011-01-01

    MicroRNA (miRNA) regulates gene expression in many cellular events,yet functions of only a few miRNAs are known in C.elegans.We analyzed the function of mir-35-41 unique to the worm,and show here that mir-35 regulates the G1/S transition of intestinal cells and germ cell proliferation.Loss of mir-35 leads to a decrease of nuclei numbers in intestine and distal mitotic gonad,while re-introduction of mir-35 rescues the mutant phenotypes.Genetic analysis indicates that mir-35 may act through Rb/E2F and SCF pathways.Further bioinformatic and functional analyses demonstrate that mir-35 targets evolutionaily conserved lin-23 and gld-1.Together,our study reveals a novel function of mir-35 family in cell division regulation.

  5. In silico Sequence Analysis, Homology Modeling and Function Annotation of Ocimum basilicum Hypothetical Protein G1CT28_OCIBA

    Directory of Open Access Journals (Sweden)

    Sobia Idrees

    2012-07-01

    Full Text Available Ocimum basilicum is commonly known as sweet basil and belongs to the Lamiaceae Family. Ocimum basilicum has great therapeutic benefits and can be used for lowering blood pressure, as an antispasmodic as well as cleansing the blood. In the present study, subcellular localization prediction suggested that it is a cytoplasmic protein. We predicted the 3D structure of protein using homology modeling as 3D structure prediction approach. 3D structure of the protein was determined using Protein Structure Prediction Server (PS2 selecting MODELLER as 3D structure prediction method. Quality analysis of the model indicated that it is a reliable model. Furthermore, it was discovered that Ocimum basilicum hypothetical protein G1CT28_OCIBA is involved in two biological processes, oxidation reduction and metabolic process and the biochemical function of the protein is acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor, catalytic activity and oxidoreductase.

  6. Optimal management for infinite capacity N-policy M/G/1 queue with a removable service station

    Science.gov (United States)

    Chang, Y. C.; Pearn, W. L.

    2011-07-01

    In this article, we consider an infinite capacity N-policy M/G/1 queueing system with a single removable server. Poisson arrivals and general distribution service times are assumed. The server is controllable that may be turned on at arrival epochs or off at service completion epochs. We apply a differential technique to study system sensitivity, which examines the effect of different system input parameters on the system. A cost model for infinite capacity queueing system under steady-state condition is developed, to determine the optimal management policy at minimum cost. Analytical results for sensitivity analysis are derived. We also provide extensive numerical computations to illustrate the analytical sensitivity properties obtained. Finally, an application example is presented to demonstrate how the model could be used in real applications to obtain the optimal management policy.

  7. A rare case of cerebral hydatidosis caused by a G1 genotype of Echinococcus granulosus in a cow from Iran.

    Science.gov (United States)

    Moazeni, M; Oryan, A; Sharifiyazdi, H; Amrabadi, O; Akbari, M

    2016-09-01

    Hydatidosis is a medically and veterinary important parasitic disease that is endemic in many parts of the world. Unilocular hydatid cysts may develop in almost any part of the body. Up to 70% of hydatid cysts are located in the liver, followed by 25% in the lungs. Cerebral hydatidosis is an uncommon manifestation of the disease, occurring in less than 1/1000 infected hosts, yet diagnosis does pose a problem. We have reported an exceptionally rare case of cerebral hydatidosis in cattle. This is the first report to describe the characteristic pathological features of the cerebral hydatidosis in cattle caused by the G1 genotype of Echinococcus granulosus. Genotypic analysis was performed on a hydatid cyst from a cow originating from southern Iran, based on the sequence analysis of the cox1 mitochondrial gene.

  8. A VLA ARCHIVE OBSERVATION OF THE YOUNGEST KNOWN GALACTIC SUPERNOVA REMNANT G1.9+0.3

    Directory of Open Access Journals (Sweden)

    Yolanda Gómez

    2009-01-01

    Full Text Available Presentamos el análisis de una observación de archivo sin publicar hecha con el Very Large Array a 1.49 GHz en 1989 hacia la remanente de supernova G1.9+0.3, la más joven conocida en la Galaxia. Esta observación concuerda con la evolución temporal del tama~no angular reportada anteriormente. Derivamos una tasa de expansión angular de 0:46 +- 0:11% por año y estimamos una edad de 220+-70/45 años comparando las imágenes de 1985 y 1989.

  9. Young Remnants of Type Ia Supernovae and Their Progenitors: A Study of SNR G1.9+0.3

    CERN Document Server

    Chakraborti, Sayan; Soderberg, Alicia

    2015-01-01

    Type Ia supernovae, with their remarkably homogeneous light curves and spectra, have been used as standardizable candles to measure the accelerating expansion of the Universe. Yet, their progenitors remain elusive. Common explanations invoke a degenerate star (white dwarf) which explodes upon reaching close to the Chandrasekhar limit, by either steadily accreting mass from a companion star or violently merging with another degenerate star. We show that circumstellar interaction in young Galactic supernova remnants can be used to distinguish between these single and double degenerate progenitor scenarios. Here we propose a new diagnostic, the Surface Brightness Index, which can be computed from theory and compared with Chandra and VLA observations. We use this method to demonstrate that a double degenerate progenitor can explain the decades-long flux rise and size increase of the youngest known Galactic SNR G1.9+0.3. We disfavor a single degenerate scenario. We attribute the observed properties to the interact...

  10. First results on the longitudinal double spin asymmetry A1p and g1p from the 2011 COMPASS data

    International Nuclear Information System (INIS)

    The COMPASS experiment at the M2 beamline of the CERN SPS has taken data with a polarized muon beam scattering of a polarized NH3 target in 2011. The beam energy has been increased to 200 GeV compared to 160 GeV in 2007. With the increased beam energy it is possible to reach higher values of Q2 and lower values of xBj, compared to our first measurement in 2007. We present our results on the longitudinal double spin asymmetry A1p and the spin-dependent structure function g1p from the 2011 data taking. This results are compared to our previously released results from 2007 and SMC.

  11. Results on the longitudinal double spin asymmetry A1p and g1p from the 2011 COMPASS data

    International Nuclear Information System (INIS)

    The COMPASS experiment at the M2 beamline of the CERN SPS has taken data with a polarized muon beam scattering of a polarized NH3 target in 2011. The beam energy has been increased to 200 GeV compared to 160 GeV in 2007. With the increased beam energy it is possible to reach higher values of Q2 and lower values of xBj, compared to our first measurement in 2007. We present our results on the longitudinal double spin asymmetry A1p and the spin-dependent structure function g1p from the 2011 data taking. These results are used in a NLO QCD fit to the world's data to obtain the polarized parton distributions and also to update our results on the validation of the Bjorken sum rule, connecting the integral of the non-singlet structure function with the ratio of the weak coupling constants.

  12. Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance

    Directory of Open Access Journals (Sweden)

    Thieffry Denis

    2009-01-01

    Full Text Available Abstract Background In breast cancer, overexpression of the transmembrane tyrosine kinase ERBB2 is an adverse prognostic marker, and occurs in almost 30% of the patients. For therapeutic intervention, ERBB2 is targeted by monoclonal antibody trastuzumab in adjuvant settings; however, de novo resistance to this antibody is still a serious issue, requiring the identification of additional targets to overcome resistance. In this study, we have combined computational simulations, experimental testing of simulation results, and finally reverse engineering of a protein interaction network to define potential therapeutic strategies for de novo trastuzumab resistant breast cancer. Results First, we employed Boolean logic to model regulatory interactions and simulated single and multiple protein loss-of-functions. Then, our simulation results were tested experimentally by producing single and double knockdowns of the network components and measuring their effects on G1/S transition during cell cycle progression. Combinatorial targeting of ERBB2 and EGFR did not affect the response to trastuzumab in de novo resistant cells, which might be due to decoupling of receptor activation and cell cycle progression. Furthermore, examination of c-MYC in resistant as well as in sensitive cell lines, using a specific chemical inhibitor of c-MYC (alone or in combination with trastuzumab, demonstrated that both trastuzumab sensitive and resistant cells responded to c-MYC perturbation. Conclusion In this study, we connected ERBB signaling with G1/S transition of the cell cycle via two major cell signaling pathways and two key transcription factors, to model an interaction network that allows for the identification of novel targets in the treatment of trastuzumab resistant breast cancer. Applying this new strategy, we found that, in contrast to trastuzumab sensitive breast cancer cells, combinatorial targeting of ERBB receptors or of key signaling intermediates does not

  13. Matrine promotes G0/G1 arrest and down-regulates cyclin D1 expression in human rhabdomyosarcoma cells.

    Science.gov (United States)

    Guo, L; Xue, T Y; Xu, W; Gao, J Z

    2013-09-01

    Matrine has a broad-spectrum of anti-cancer effects and is efficient in the inhibition of proliferation of hepatoma cells, leukemia cells and neuroblastoma cell. However, its efficacy and tentative mechanisms in rhabdomyosarcoma have not been addressed before. This study aimed to investigate the effects of Matrine on cell cycle and expression of cyclin D1 in human rhabdomyosarcoma cells (RD cell line). RD cell line was treated with different concentrations (0, 0.5, 1.0, and 1.5 mg/mL) of Matrine, and cell proliferation and cell cycle were evaluated using, respectively, MTT assay and flow cytometry. The effect of Matrine on cyclin D1 mRNA levels was measured by RT-PCR. There was a dose-dependent inhibition of proliferation in the matrine-treated group (inhibition of proliferation rate in control cells 12.70 ± 0.35%; Matrine-treated cells [0.5, 1.0, and 1.5 mg/mL]: 31.16 ± 0.11%, 42.96 ± 0.9%, and 57.26 ± 0.8%). The G0 / G1 ratio in study groups were, respectively, 58.44 ± 3.57%, 64.79 ± 2.03%, 69.97 ± 2.89% and 75.03 ± 1.23%.Cyclin D1 mRNA levels progressively diminished (control group ratio of cyclin D1 / β-actin: 0.59 ± 0.06; Matrine: 0.35 ± 0.05, 0.27 ± 0.02 and 0.04 ± 0.03). All aforementioned changes were significant (PMatrine markedly suppresses cell proliferation in RD cells by decreasing expression of cyclin D1 mRNA and blocking the cell cycle at the G0 / G1 stage.

  14. Accurate Analytic Potential Energy Function and Spectroscopic Study for G1 ∏g State of Dimer 7Li2

    Institute of Scientific and Technical Information of China (English)

    SHI De-Heng; MA Heng; SUN Jin-Feng; ZHU Zun-Lue

    2007-01-01

    The reasonable dissociation limit for the G1∏g state of dimer 7Li2 is determined. The equilibrium internuclear distance, dissociation energy, harmonic frequency, vibrational zero energy, and adiabatic excitation energy are calculated using a symmetry-adapted-cluster configuration-interaction method in complete active space in Gaussian03 program package at such numerous basis sets as 6-311++G, 6-311++G(2df, 2pd), 6-311++G(2df, p), cc-PVTZ, 6-311++G(3df, 3pd), CEP-121G, 6-311++G(2df, pd), 6-311++G(d,p),6-311G(3df,3pd), D95(3df,3pd), 6-311++G(3df, 2p),6-311++G(2df), 6-311++G(df, pd) D95V++, and DGDZVP. The complete potential energy curves are obtained at these sets over a wide internuclear distance range and have least squares fitted to Murrell-Sorbie function. The conclusion shows that the basis set 6-311++G(2df, p) is a most suitable one for the G1∏g state. At this basis set, the calculated spectroscopic constants Te, De, Eo, Re, ωe, ωeXe, αe, and Be are of 3.9523 eV, 0.813 06 eV, 113.56 cm-1, 0.320 15 nm,227.96 cm-1, 1.6928 cm-1, 0.004 436 cm-1, and 0.4689 cm-1, respectively, which are in good agreement with measurements whenever available. The total 50 vibrational levels and corresponding inertial rotation constants are for the first time calculated and compared with available RKR data. And good agreement with measurements is obtained.

  15. Identificación de los polimorfismos G1 y G8 del gen GDF9 en ovinos criollos Araucanos

    Directory of Open Access Journals (Sweden)

    E Paz

    2014-01-01

    Full Text Available En la especie ovina se han descrito una serie de polimorfismos en genes de efecto mayor relacionados con la actividad reproductiva. Las mutaciones ubicadas en el gen de efecto mayor GDF9 se han asociado con el incremento de la tasa ovulatoria y el tamaño de la camada. GDF9 es un factor celular secretado por el ovocito y es miembro de la familia de factores de crecimiento transformante (TGF-β localizado en el cromosoma 5 ovino. El objetivo de este trabajo fue identificar la presencia de los polimorfismos en los sitios G1 y G8 en el gen GDF9 en ovinos criollos Araucanos. Se extrajo el ADN de 100 muestras sanguíneas para posteriormente determinar la presencia de las mutaciones utilizando la técnica PCR-RFLP. Fue amplificada una región de 462 pb correspondiente al exón 1, la cuál fue digerida con la enzima de restricción HhaI, el siguiente fragmento amplificado fue de 139 pb correspondiente al exón 2, siendo digerido con la enzima Ddel. Se identificó la presencia del polimorfismo G1 con una frecuencia genotípica del 0,56 (genotipo GG, 0,44 (genotipo GA y una frecuencia alélica de 0,78 para el alelo (G y 0,22 para el alelo (A. No se detectó la presencia de polimorfismo G8. Este es el primer reporte de este polimorfismo en ovinos en Chile que podría servir como un marcador genético de prolificidad para la selección de ovinos criollos Araucanos.

  16. The measurement of g1n polarized structure of the neutron by the E154 experiment at SLAC

    International Nuclear Information System (INIS)

    This thesis presents the precision measurement of the neutron polarized structure g1n performed by the E154 collaboration at the Standford Linear Accelerator Center, USA, in autumn 1995, using a 48.3 GeV polarized electron beam scattered off a polarized Helium 3 target. The scattered electrons were detected using two spectrometer arms, covering the deep inelastic scattering range: 0.0134 2 2 2 at an average value of Q2 = 5 GeV2. Two electromagnetic calorimeters have been designed by the LPC in Clermont-Ferrand and the SphN-CEA in Saclay to measure the scattered electron energy and to eject the contaminating hadron background using, a cellular automaton and a neural network, widely described in this thesis. The analysis performed in Clermont-Ferrand and presented in this document led us to the integral on the measurement region of g1n equaling: - 0.34 ± 0.003STAT ± 0.004SYST ± 0.001EVOL at Q2 = 5 GeV2, where our data have been evolved to Q2 = 5 GeV2 using the next-to-leading order DGLAP evolution equations and a world parametrization of the polarized parton distributions. The Ellis and Jaffe sum rule is clearly violated. Using different low x extrapolations, our integral is compatible with the Bjorken sum rule. The quark contribution to the nucleon spin is ΔΣ = 29 ± 6 % in the M S-bar scheme and ΔΣ = 37 ± 7% in the AB scheme, at Q2 = 5 GeV2. The gluon contribution seems to be positive and within the range: 0 < ΔG < 2. (author)

  17. 带启动-关闭期的多重休假M/G/1排队系统的队长分布%The Queue-Length Distribution For M/G/1 Queueing System with Startup -Close Periods and Multiple Vacations

    Institute of Scientific and Technical Information of China (English)

    蒲会; 唐应辉

    2011-01-01

    研究带启动-关闭期的多重休假M/G/1排队系统,讨论了队长的瞬态和稳态性质.通过引进的"服务员忙期"和使用全概率分解技术,导出了在任意时刻t队长的瞬态分布的L变换的递推表达式和稳态队长分布的递推表达式,以及稳态队长的随机分解结果.%In this paper the M/G/1 queueing system with startup-close periods and multiple vacations is considered. We study the transient and equilibrium properties of the queuelength. By introducing the server busy period and using the total probability decomposition technique, we derive the recursion expression of the L-transformation of the transient queuelength distribution at any time t, and also the recursion expressions of the distribution and stochastic decomposition of the queue-length at a random point in equilibrium.

  18. 基于G1-DEMATEL的飞行员心理健康评价指标体系研究%Research of Pilot Mental Health Evaluation Index System Based on G1-DEMATEL Method

    Institute of Scientific and Technical Information of China (English)

    李敬强; 赵宁

    2014-01-01

    目的 构建民航飞行员心理健康评价指标体系,并确定其权重分布.方法 从心理健康的理论出发,构建心理健康评价体系,结合心理健康标准与飞行员的实际情况,建立民航飞行员的心理健康评价指标体系,结合G1-DEMATEL法计算指标权重分布.结果 民航飞行员的心理健康评价指标体系由发展性与适应性两部分组成,共14个指标,得到了相应的权重分布,以数学表达式表示了发展性、适应性与二者协调性指数三个参数的心理健康判断依据,并根据DEMATEL的关联度得到了指标间相互影响关系.结论 民航飞行员心理健康评价指标体系的建立将为飞行员心理健康测评提供指标与权重上的支撑.%Objective To establish systematically mental health evaluation index system for civil aviation pilots and determine its weight distribution.Methods Proceeded from mental health theory,the evaluation system about mental health was established.Then,combined with the standards about mental health and the actual situation of pilots,mental health evaluation index system of civil pilots was built.Finally,its weight distribution was calculated with G1-DEMATEL methods.Results The evaluation index system of civil pilot mental health was consisted of development and adaptability,including 14 indicators.Its weight distribution was obtained.The criteria of mental health were represented by mathematical expressions as three parameters about development (D),adaptability(A) and coordination of both(C).In addition,the interaction relationship among indicators was shown with correlation degree of DEMATEL method.Conclusion The establishment of mental health evaluation index system for civil pilots will provide supports on the index and weight for assessment of mental health of pilots.

  19. [Comparative visual field study using the Octopus 2,000R with the global analysis program G1 with the grid pattern].

    Science.gov (United States)

    Kieselbach, G F; Juen, S

    1988-01-01

    At the Universitäts-Augenklinik Innsbruck patients with diabetic macular edema are treated with grid pattern since February 1986. Thirty-two eyes from 18 patients were tested with the program G1 on perimeter Octopus 2000R before and after grid pattern. The evaluated parameters presented by the program G1 are discussed. PMID:3362513

  20. Bangladesh; Statistical Appendix

    OpenAIRE

    International Monetary Fund

    1995-01-01

    The paper presents statistical data on gross domestic product, agricultural production, manufacturing production, energy statistics, retail prices of petroleum products, consumer price index, central government operations, revenues, and central expenditure in Bangladesh. It also presents the central government food account, consolidated accounts and profits of nonfinancial public enterprises, government domestic securities, monetary survey, balance sheet of the monetary authorities, interest ...

  1. Structure verification of a recombinant chimeric anti-CD20 IgG1 monoclonal antibody%重组嵌合抗CD20 IgG1型单克隆抗体的结构验证

    Institute of Scientific and Technical Information of China (English)

    陶磊; 饶春明; 高凯; 史新昌; 赵阳; 王军志

    2010-01-01

    本文选择一种重组嵌合抗CD20 IgG1型单抗.应用液质联用仪及N-末端测序仪对其进行结构验证.对该单抗进行还原、烷基化、酶解等处理后,对其氨基酸序列、二硫键配对方式、糖链类型及糖基化位点进行分析测定.结果显示,该单抗轻、重链氨基酸序列与理论一致.通过液质肽图的解析,对单抗10条二硫键的配对方式进行了验证;通过比较单抗重链切糖前、后的相对分子质量,预测单抗所含糖链类型为岩藻糖化的双触角复杂型N-糖,糖基化位点位于重链的Asn301上.本方法可为该类重组单抗制品的质量控制及其参考品的结构确证提供参考.

  2. Indonesia; Statistical Appendix

    OpenAIRE

    International Monetary Fund

    1999-01-01

    The statistical data on developments in gross domestic product by sector of origin and by expenditure, agricultural production, production, domestic use, and exports of petroleum, approvals of foreign investment projects by economic sector, indices of inflation, consumer price index, summary of central government operations, and central government revenue of Indonesia are presented in the paper. The data on details of nontax receipts, Bank Indonesia liquidity support, foreign exchange and equ...

  3. Efficient paper recycling

    OpenAIRE

    Gregor-Svetec, Diana; Možina, Klemen; Blaznik, Barbara; Urbas, Raša; Vrabič Brodnjak, Urška; Golob, Gorazd

    2013-01-01

    Used paper and paper products are important raw material for paper and board industry. Paper recycling increases the material lifespan and is a key strategy that contributes to savings of primary raw material, reduction of energy and chemicals consumption, reduction of the impact on fresh water and improvement of waste management strategies. The paper recycling rate is still highly inhomogeneous among the countries of Central Europe. Since recovered paper is not only recycled in the country w...

  4. 40 CFR Appendix W to Part 51 - Guideline on Air Quality Models

    Science.gov (United States)

    2010-07-01

    ....0Bibliography 12.0References Appendix A to Appendix W of 40 CFR Part 51—Summaries of Preferred Air Quality... assessing source impact and in evaluating control strategies. i. Appendix W to 40 CFR Part 51 itself... to Appendix A to Appendix W to 40 CFR Part 51. Appendix A contains summaries of refined air...

  5. 具有反馈、不可靠服务台和二次多选择服务M/G/1重试排队系统%An M/G/1 Retrial Queue with Second Muilti-Optional Service Feedback and Unreliable Server

    Institute of Scientific and Technical Information of China (English)

    李江华; 王金亭

    2006-01-01

    An M/G/1 retrial queue with two-phase service and feedback is studied in this paper, where the server is subject to starting failures and breakdowns during service. Primary customers get in the system according to a Poisson process, and they will receive service immediately if the server is available upon arrival. Otherwise, they will enter a retrial orbit and are queued in the orbit in accordance with a first-come-first-served (FCFS) discipline. Customers are allowed to balk and renege at particular times. All customers demand the first "essential"service, whereas only some of them demand the second "multi-optional" service. It is assumed that the retrial time, service time and repair time of the server are all arbitrarily distributed.The necessary and sufficient condition for the system stability is derived. Using a supplementary variable method, the steady-state solutions for some queueing and reliability measures of the system are obtained.

  6. Regulation of G0/G1 switch gene 2 (G0S2) expression in human adipose tissue.

    Science.gov (United States)

    Skopp, Alexander; May, Marcus; Janke, Juergen; Kielstein, Heike; Wunder, Ruth; Flade-Kuthe, Ricarda; Kuthe, Andreas; Jordan, Jens; Engeli, Stefan

    2016-05-01

    The G0/G1 switch gene 2 (G0S2) protein attenuated adipose triglyceride lipase (ATGL) activity and decreased lipolysis in rodent and human adipocytes. We hypothesized that G0S2 mRNA expression in human adipose tissue is influenced by depot, adipocyte size, body weight and caloric intake. Adipose tissue samples were obtained during abdominal surgery and by needle biopsy before and 3 h after an extended glucose load in lean subjects. G0S2 mRNA was 7× higher expressed in mature human adipocytes compared to the stromavascular fraction. Cell size inversely correlated with G0S2 mRNA expression in both, subcutaneous and omental adipose depots. G0S2 mRNA expression was 75% higher in subcutaneous compared to omental adipose tissue. Obesity was associated with lower G0S2 mRNA expression in subcutaneous adipose tissue. Acute glucose ingestion after an overnight fast did not significantly increase G0S2 expression in subcutaneous adipose tissue. In conclusion, differences in G0S2 expression may explain depot-specific and obesity-associated differences in lipolysis on the molecular level. PMID:26707160

  7. DO COMETS C/1861 G1 (THATCHER AND C/1861 J1 (GREAT COMET HAVE A COMMON ORIGIN?

    Directory of Open Access Journals (Sweden)

    Richard L. Branham

    2015-01-01

    Full Text Available Con el objeto de mejorar la ́orbita de Oppolzer de 1864, se cal cula una nueva ́orbita para el Cometa C/1861 G1 (Thatcher, el cual es t ́a asociado a la lluvia de estrellas de las L ́ıridas. La nueva ́orbita se basa en 649 observaciones hechas entre el 11 de abril y el 7 de septiembre de 1861, 326 en a scensi ́on recta y 323 en declinaci ́on. La ́orbita final utiliza los residuos ca lculados con la funci ́on de ponderaci ́on de Welsch. El per ́ıodo del cometa, 416 ± 0.56 a ̃nos, concuerda con el de Oppolzer, 415 a ̃nos, pero otros elementos orbitale s, como la inclinaci ́on, discrepan. Si bien los residuos post-perihelio se presenta n relativamente al azar, con una probabilidad del 52.1% de serlo, los residuos pre-perih elio no son azarosos, lo cual indica posibles desviaciones del movimiento kepleria no debidas a la expulsi ́on de material meteor ́ıtico. El Cometa Thatcher no est ́a relac ionado con el Gran Cometa de 1861.

  8. CRIF1 interacting with CDK2 regulates bone marrow microenvironment-induced G0/G1 arrest of leukemia cells.

    Directory of Open Access Journals (Sweden)

    Qian Ran

    Full Text Available BACKGROUND: To assess the level of CR6-interacting factor 1 (CRIF1, a cell cycle negative regulator, in patients with leukemia and investigate the role of CRIF1 in regulating leukemia cell cycle. METHODS: We compared the CRIF1 level in bone marrow (BM samples from healthy and acute myeloid leukemia (AML, iron deficiency anemia (IDA and AML-complete remission (AML-CR subjects. We also manipulated CRIF1 level in the Jurkat cells using lentivirus-mediated overexpression or siRNA-mediated depletion. Co-culture with the BM stromal cells (BMSCs was used to induce leukemia cell cycle arrest and mimic the BM microenvironment. RESULTS: We found significant decreases of CRIF1 mRNA and protein in the AML group. CRIF1 overexpression increased the proportion of Jurkat cells arrested in G0/G1, while depletion of endogenous CRIF1 decreased cell cycle arrest. Depletion of CRIF1 reversed BMSCs induced cell cycle arrest in leukemia cells. Co-immunoprecipitation showed a specific binding of CDK2 to CRIF1 in Jurkat cells during cell cycle arrest. Co-localization of two proteins in both nucleus and cytoplasm was also observed with immunofluorescent staining. CONCLUSION: CRIF1 may play a regulatory role in the BM microenvironment-induced leukemia cell cycle arrest possibly through interacting with CDK2 and acting as a cyclin-dependent kinase inhibitor.

  9. Studies on Middle-Phase Microemulsions of Green Surfactant n -Dodecyl Polyglucoside C12G1.46

    Institute of Scientific and Technical Information of China (English)

    CHAI,Jin-Ling; WU,Chang-Ju; LI,Gan-Zuo; ZHANG,Gao-Yong

    2003-01-01

    The three-phase behavior in the quaternary system ot n-dodecyl polyglucoside C12G1.46/1-butanol/cyclohexane/water has been studied at 40 ℃ in terms of the variables γ and δ. Increasing δ at constant γ causes a phase inversion from an oil-in-water microemulsion in contact with excess oil ( winsor Ⅰ or 2) to a water-in-oil microemulsion in contact with excess water (winsor Ⅱ or 2) via a middle-phase microemulsion in contact with excess oil and water (winsor Ⅲ or 3). By taking into account the different solubilities of alkyl polyglucoside and 1-butanol in the oil phase, the composition of the hydrophile-lipophile balanced interfacial film in the middle of the three-phase body can be calculated. The effects of different oils and aqueous media on the phase behavior and on the composition of the interfacial film and the efficiency for alkyl polyglucoside to make equal weights of water and oil to a single phase were investigated.It was found that the oil molecules with small molecular volumes can improve the solubilizing efficiency of the surfactant to form single-phase microemulsion. In inorganic salt (NaCl) and acid (HCl)solutions, less 1-butanol is needed than that in alkali (NaOH) solution to form middle-phase microemulsion.

  10. MicroRNA-206 induces G1 arrest in melanoma by inhibition of CDK4 and Cyclin D.

    Science.gov (United States)

    Georgantas, Robert W; Streicher, Katie; Luo, Xiaobing; Greenlees, Lydia; Zhu, Wei; Liu, Zheng; Brohawn, Philip; Morehouse, Christopher; Higgs, Brandon W; Richman, Laura; Jallal, Bahija; Yao, Yihong; Ranade, Koustubh

    2014-03-01

    Expression profiling of microRNAs in melanoma lesional skin biopsies compared with normal donor skin biopsies, as well as melanoma cell lines compared with normal melanocytes, revealed that hsa-miR-206 was down-regulated in melanoma (-75.4-fold, P = 1.7 × 10(-4)). MiR-206 has been implicated in a large number of cancers, including breast, lung, colorectal, ovarian, and prostate cancers; however, its role in tumor development remains largely unknown, its biologic function is poorly characterized, and its targets affecting cancer cells are largely unknown. MiR-206 reduced growth and migration/invasion of multiple melanoma cell lines. Bioinformatics identified cell cycle genes CDK2, CDK4, Cyclin C, and Cyclin D1 as strong candidate targets. Western blots and 3'UTR reporter gene assays revealed that miR-206 inhibited translation of CDK4, Cyclin D1, and Cyclin C. Additionally, hsa-miR-206 transfection induced G1 arrest in multiple melanoma cell lines. These observations support hsa-miR-206 as a tumor suppressor in melanoma and identify Cyclin C, Cyclin D1, and CDK4 as miR-206 targets. PMID:24289491

  11. Inhibition of G1P3 expression found in the differential display study on respiratory syncytial virus infection

    Directory of Open Access Journals (Sweden)

    Li Lei

    2008-10-01

    Full Text Available Abstract Background Respiratory syncytial virus (RSV is the leading viral pathogen associated with bronchiolitis and lower respiratory tract disease in infants and young children worldwide. The respiratory epithelium is the primary initiator of pulmonary inflammation in RSV infections, which cause significant perturbations of global gene expression controlling multiple cellular processes. In this study, differential display reverse transcription polymerase chain reaction amplification was performed to examine mRNA expression in a human alveolar cell line (SPC-A1 infected with RSV. Results Of the 2,500 interpretable bands on denaturing polyacrylamide gels, 40 (1.6% cDNA bands were differentially regulated by RSV, in which 28 (70% appeared to be upregulated and another 12 (30% appeared to be downregulated. Forty of the expressed sequence tags (EST were isolated, and 20 matched homologs in GenBank. RSV infection upregulated the mRNA expression of chemokines CC and CXC and interfered with type α/β interferon-inducible gene expression by upregulation of MG11 and downregulation of G1P3. Conclusion RSV replication could induce widespread changes in gene expression including both positive and negative regulation and play a different role in the down-regulation of IFN-α and up-regulation of IFN-γ inducible gene expression, which suggests that RSV interferes with the innate antiviral response of epithelial cells by multiple mechanisms.

  12. The G1/S Specific Cyclin D2 Is a Regulator of HIV-1 Restriction in Non-proliferating Cells.

    Science.gov (United States)

    Badia, Roger; Pujantell, Maria; Riveira-Muñoz, Eva; Puig, Teresa; Torres-Torronteras, Javier; Martí, Ramón; Clotet, Bonaventura; Ampudia, Rosa M; Vives-Pi, Marta; Esté, José A; Ballana, Ester

    2016-08-01

    Macrophages are a heterogeneous cell population strongly influenced by differentiation stimuli that become susceptible to HIV-1 infection after inactivation of the restriction factor SAMHD1 by cyclin-dependent kinases (CDK). Here, we have used primary human monocyte-derived macrophages differentiated through different stimuli to evaluate macrophage heterogeneity on cell activation and proliferation and susceptibility to HIV-1 infection. Stimulation of monocytes with GM-CSF induces a non-proliferating macrophage population highly restrictive to HIV-1 infection, characterized by the upregulation of the G1/S-specific cyclin D2, known to control early steps of cell cycle progression. Knockdown of cyclin D2, enhances HIV-1 replication in GM-CSF macrophages through inactivation of SAMHD1 restriction factor by phosphorylation. Co-immunoprecipitation experiments show that cyclin D2 forms a complex with CDK4 and p21, a factor known to restrict HIV-1 replication by affecting the function of the downstream cascade that leads to SAMHD1 deactivation. Thus, we demonstrate that cyclin D2 acts as regulator of cell cycle proteins affecting SAMHD1-mediated HIV-1 restriction in non-proliferating macrophages. PMID:27541004

  13. A detailed analysis of cyclin A accumulation at the G(1)/S border in normal and transformed cells.

    Science.gov (United States)

    Erlandsson, F; Linnman, C; Ekholm, S; Bengtsson, E; Zetterberg, A

    2000-08-25

    The temporal relationship between cyclin A accumulation and the onset of DNA replication was analyzed in detail. Five untransformed and nine transformed asynchronously growing cell cultures were investigated using a triple immunofluorescence staining protocol combined with computerized evaluation of staining intensities in individual cells. The simultaneous staining of BrdU, cyclin A, and cyclin E made it possible to determine the cell cycle position of each cell investigated. Cells at the G(1)/S border were identified on the basis of cyclin E content and were further analyzed with respect to cyclin A and BrdU content. A method was developed to calculate objective thresholds defining the highest staining intensity found in the negative cells in the population. Using the thresholds we could distinguish cells with minute amounts of cyclin A and BrdU from truly negative cells. We show that the onset of cyclin A accumulation and the start of DNA replication occurs at the same time, or deviating by a few minutes at the most. We also show that cyclin A accumulates continuously during S. This study clearly demonstrates that nuclear cyclin A can be used as a reliable marker for the S and G(2) phases in both normal and transformed interphase cells. PMID:10942581

  14. CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest.

    Science.gov (United States)

    Pan, Wen; Cheng, Yingying; Zhang, Heyu; Liu, Baocai; Mo, Xiaoning; Li, Ting; Li, Lin; Cheng, Xiaojing; Zhang, Lianhai; Ji, Jiafu; Wang, Pingzhang; Han, Wenling

    2014-01-01

    Cytokines are soluble proteins that exert their functions by binding specific receptors. Many cytokines play essential roles in carcinogenesis and have been developed for the treatment of cancer. In this study, we identified a novel potential cytokine using immunogenomics designated colon-derived SUSD2 binding factor (CSBF), also known as chromosome 10 open reading frame 99 (C10orf99). CSBF/C10orf99 is a classical secreted protein with predicted molecular mass of 6.5 kDa, and a functional ligand of Sushi Domain Containing 2 (SUSD2). CSBF/C10orf99 has the highest expression level in colon tissue. Both CSBF/C10orf99 and SUSD2 are down-regulated in colon cancer tissues and cell lines with different regulation mechanisms. CSBF/C10orf99 interacts with SUSD2 to inhibit colon cancer cell growth and induce G1 cell cycle arrest by down-regulating cyclin D and cyclin-dependent kinase 6 (CDK6). CSBF/C10orf99 displays a bell-shaped activity curve with the optimal effect at ~10 ng/ml. Its growth inhibitory effects can be blocked by sSUSD2-Fc soluble protein. Our results suggest that CSBF/C10orf99 is a novel potential cytokine with tumor suppressor functions. PMID:25351403

  15. Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution.

    Science.gov (United States)

    Traxlmayr, Michael W; Lobner, Elisabeth; Hasenhindl, Christoph; Stadlmayr, Gerhard; Oostenbrink, Chris; Rüker, Florian; Obinger, Christian

    2014-08-01

    For most therapeutic proteins, a long serum half-life is desired. Studies have shown that decreased antigen binding at acidic pH can increase serum half-life. In this study, we aimed to investigate whether pH-dependent binding sites can be introduced into antigen binding crystallizable fragments of immunoglobulin G1 (Fcab). The C-terminal structural loops of an Fcab were engineered for reduced binding to the extracellular domain of human epidermal growth factor receptor 2 (Her2-ECD) at pH 6 compared to pH 7.4. A yeast-displayed Fcab-library was alternately selected for binding at pH 7.4 and non-binding at pH 6.0. Selected Fcab variants showed clear pH-dependent binding to soluble Her2-ECD (decrease in affinity at pH 6.0 compared to pH 7.4) when displayed on yeast. Additionally, some solubly expressed variants exhibited pH-dependent interactions with Her2-positive cells whereas their conformational and thermal stability was pH-independent. Interestingly, two of the three Fcabs did not contain a single histidine mutation but all of them contained variations next to histidines that already occurred in loops of the lead Fcab. The study demonstrates that yeast surface display is a valuable tool for directed evolution of pH-dependent binding sites in proteins. PMID:24964247

  16. Resibufogenin Induces G1-Phase Arrest through the Proteasomal Degradation of Cyclin D1 in Human Malignant Tumor Cells.

    Directory of Open Access Journals (Sweden)

    Masami Ichikawa

    Full Text Available Huachansu, a traditional Chinese medicine prepared from the dried toad skin, has been used in clinical studies for various cancers in China. Resibufogenin is a component of huachansu and classified as bufadienolides. Resibufogenin has been shown to exhibit the anti-proliferative effect against cancer cells. However, the molecular mechanism of resibufogenin remains unknown. Here we report that resibufogenin induces G1-phase arrest with hypophosphorylation of retinoblastoma (RB protein and down-regulation of cyclin D1 expression in human colon cancer HT-29 cells. Since the down-regulation of cyclin D1 was completely blocked by a proteasome inhibitor MG132, the suppression of cyclin D1 expression by resibufogenin was considered to be in a proteasome-dependent manner. It is known that glycogen synthase kinase-3β (GSK-3β induces the proteasomal degradation of cyclin D1. The addition of GSK-3β inhibitor SB216763 inhibited the reduction of cyclin D1 caused by resibufogenin. These effects on cyclin D1 by resibufogenin were also observed in human lung cancer A549 cells. These findings suggest that the anti-proliferative effect of resibufogenin may be attributed to the degradation of cyclin D1 caused by the activation of GSK-3β.

  17. High glucose decreases the expression of ATP-binding cassette transporter G1 in human vascular smooth muscle cells

    Institute of Scientific and Technical Information of China (English)

    Jiahong Xue; Zuyi Yuan; Yue Wu; Yan Zhao; Zhaofei Wan

    2008-01-01

    Objective:ATP-binding cassette transporters(ABC) A1 and G1 play an important role in mediating cholesterol efflux and preventing macrophage foam cell formation. In this study, we examined the regulation of ABC transporters by high glucose in human vascular smooth muscle cells(VSMCs), the other precursor of foam cells. Methods:Incubation of human VSMCs with D-ghicose(5 to 30 mM) for 1 to 7 days in the presence or absence of antioxidant and nuclear factor(NF)-kB inhibitors, the expressions of ABCA1 and ABCG1 were analyzed by real time PCR and Western blotting. Results:High glucose decreased ABCG1 mRNA and protein expression in cultured VSMCs, whereas the expression of ABCA1 was not significantly decreased. Down-regulation of ABCG1 mRNA expression by high glucose was abolished by antioxidant N-acetyl-L-cysteine(NAC) and NF-kB inhibitors, BAY 11-7085 and tosyl-phenylalanine chloromethyl-ketone(TPCK). Conclusion:High glucose suppresses the expression of ABCG1 in VSMCs, which is the possible mechanism of VSMC derived foam cell transformation.

  18. MicroRNA-206 induces G1 arrest in melanoma by inhibition of CDK4 and Cyclin D.

    Science.gov (United States)

    Georgantas, Robert W; Streicher, Katie; Luo, Xiaobing; Greenlees, Lydia; Zhu, Wei; Liu, Zheng; Brohawn, Philip; Morehouse, Christopher; Higgs, Brandon W; Richman, Laura; Jallal, Bahija; Yao, Yihong; Ranade, Koustubh

    2014-03-01

    Expression profiling of microRNAs in melanoma lesional skin biopsies compared with normal donor skin biopsies, as well as melanoma cell lines compared with normal melanocytes, revealed that hsa-miR-206 was down-regulated in melanoma (-75.4-fold, P = 1.7 × 10(-4)). MiR-206 has been implicated in a large number of cancers, including breast, lung, colorectal, ovarian, and prostate cancers; however, its role in tumor development remains largely unknown, its biologic function is poorly characterized, and its targets affecting cancer cells are largely unknown. MiR-206 reduced growth and migration/invasion of multiple melanoma cell lines. Bioinformatics identified cell cycle genes CDK2, CDK4, Cyclin C, and Cyclin D1 as strong candidate targets. Western blots and 3'UTR reporter gene assays revealed that miR-206 inhibited translation of CDK4, Cyclin D1, and Cyclin C. Additionally, hsa-miR-206 transfection induced G1 arrest in multiple melanoma cell lines. These observations support hsa-miR-206 as a tumor suppressor in melanoma and identify Cyclin C, Cyclin D1, and CDK4 as miR-206 targets.

  19. CDK1-Cyclin B1 Activates RNMT, Coordinating mRNA Cap Methylation with G1 Phase Transcription.

    Science.gov (United States)

    Aregger, Michael; Kaskar, Aneesa; Varshney, Dhaval; Fernandez-Sanchez, Maria Elena; Inesta-Vaquera, Francisco A; Weidlich, Simone; Cowling, Victoria H

    2016-03-01

    The creation of translation-competent mRNA is dependent on RNA polymerase II transcripts being modified by addition of the 7-methylguanosine (m7G) cap. The factors that mediate splicing, nuclear export, and translation initiation are recruited to the transcript via the cap. The cap structure is formed by several activities and completed by RNMT (RNA guanine-7 methyltransferase), which catalyzes N7 methylation of the cap guanosine. We report that CDK1-cyclin B1 phosphorylates the RNMT regulatory domain on T77 during G2/M phase of the cell cycle. RNMT T77 phosphorylation activates the enzyme both directly and indirectly by inhibiting interaction with KPNA2, an RNMT inhibitor. RNMT T77 phosphorylation results in elevated m7G cap methyltransferase activity at the beginning of G1 phase, coordinating mRNA capping with the burst of transcription that occurs following nuclear envelope reformation. RNMT T77 phosphorylation is required for the production of cohort of proteins, and inhibiting T77 phosphorylation reduces the cell proliferation rate. PMID:26942677

  20. Effects of Sterigmatocystin, Deoxynivalenol and Aflatoxin G1 on Apoptosis of Human Peripheral Blood Lymphocytes in vitro

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Objective To explore the effects of Sterigmatocystin (ST), Deoxynivalenol (DON) and Aflatoxin G1 (AFG1) on apoptosis of human peripheral blood lymphocytes (HPBLs) in vitro and thus to further elucidate the putative roles of these three mycotoxins on human immunosystem. Methods The effects of ST, DON and AFG1 on apoptosis of HPBLs were studied with cell culture, flow cytometric (FCM) DNA analysis and DNA agarose gel electrophoresis. Results DNA agarose gel electrophoresis results showed the characteristic "ladder" pattern of apoptosis in HPBLs treated with ST, DON and AFG1. Flow cytometric DNA analysis revealed that typical subdiploid peaks of apoptosis in DNA histogram could be seen in all groups treated with the three mycotoxins. Significant time-effect and dose-effect relationships were found between the apoptosis rates and treatment time as well as concentrations of the three mycotoxins. Conclusion ST, DON and AFG1 can induce apoptosis of HPBLs in vitro and may have some negative effects on human immunosystem.

  1. Loss of lysosomal membrane protein NCU-G1 in mice results in spontaneous liver fibrosis with accumulation of lipofuscin and iron in Kupffer cells

    Directory of Open Access Journals (Sweden)

    Xiang Y. Kong

    2014-03-01

    Full Text Available Human kidney predominant protein, NCU-G1, is a highly conserved protein with an unknown biological function. Initially described as a nuclear protein, it was later shown to be a bona fide lysosomal integral membrane protein. To gain insight into the physiological function of NCU-G1, mice with no detectable expression of this gene were created using a gene-trap strategy, and Ncu-g1gt/gt mice were successfully characterized. Lysosomal disorders are mainly caused by lack of or malfunctioning of proteins in the endosomal-lysosomal pathway. The clinical symptoms vary, but often include liver dysfunction. Persistent liver damage activates fibrogenesis and, if unremedied, eventually leads to liver fibrosis/cirrhosis and death. We demonstrate that the disruption of Ncu-g1 results in spontaneous liver fibrosis in mice as the predominant phenotype. Evidence for an increased rate of hepatic cell death, oxidative stress and active fibrogenesis were detected in Ncu-g1gt/gt liver. In addition to collagen deposition, microscopic examination of liver sections revealed accumulation of autofluorescent lipofuscin and iron in Ncu-g1gt/gt Kupffer cells. Because only a few transgenic mouse models have been identified with chronic liver injury and spontaneous liver fibrosis development, we propose that the Ncu-g1gt/gt mouse could be a valuable new tool in the development of novel treatments for the attenuation of fibrosis due to chronic liver damage.

  2. Metabolism of methoxychlor by the P450-monooxygenase CYP6G1 involved in insecticide resistance of Drosophila melanogaster after expression in cell cultures of Nicotiana tabacum.

    Science.gov (United States)

    Joussen, Nicole; Schuphan, Ingolf; Schmidt, Burkhard

    2010-03-01

    Cytochrome P450 monooxygenase CYP6G1 of Drosophila melanogaster was heterologously expressed in a cell suspension culture of Nicotiana tabacum. This in vitro system was used to study the capability of CYP6G1 to metabolize the insecticide methoxychlor (=1,1,1-trichloro-2,2-bis(4-methoxyphenyl)ethane, 1) against the background of endogenous enzymes of the corresponding non-transgenic culture. The Cyp6g1-transgenic cell culture metabolized 96% of applied methoxychlor (45.8 microg per assay) within 24 h by demethylation and hydroxylation mainly to trishydroxy and catechol methoxychlor (16 and 17%, resp.). About 34% of the metabolism and the distinct formation of trishydroxy and catechol methoxychlor were due to foreign enzyme CYP6G1. Furthermore, methoxychlor metabolism was inhibited by 43% after simultaneous addition of piperonyl butoxide (458 microg), whereas inhibition in the non-transgenic culture amounted to 92%. Additionally, the rate of glycosylation was reduced in both cultures. These results were supported by the inhibition of the metabolism of the insecticide imidacloprid (6; 20 microg, 24 h) in the Cyp6g1-transgenic culture by 82% in the presence of piperonyl butoxide (200 microg). Due to CYP6G1 being responsible for imidacloprid resistance of Drosophila or being involved in DDT resistance, it is likely that CYP6G1 conveys resistance to methoxychlor (1). Furthermore, treating Drosophila with piperonyl butoxide could weaken the observed resistance phenomena.

  3. Evolutionary changes in gene expression, coding sequence and copy-number at the Cyp6g1 locus contribute to resistance to multiple insecticides in Drosophila.

    Directory of Open Access Journals (Sweden)

    Thomas W R Harrop

    Full Text Available Widespread use of insecticides has led to insecticide resistance in many populations of insects. In some populations, resistance has evolved to multiple pesticides. In Drosophila melanogaster, resistance to multiple classes of insecticide is due to the overexpression of a single cytochrome P450 gene, Cyp6g1. Overexpression of Cyp6g1 appears to have evolved in parallel in Drosophila simulans, a sibling species of D. melanogaster, where it is also associated with insecticide resistance. However, it is not known whether the ability of the CYP6G1 enzyme to provide resistance to multiple insecticides evolved recently in D. melanogaster or if this function is present in all Drosophila species. Here we show that duplication of the Cyp6g1 gene occurred at least four times during the evolution of different Drosophila species, and the ability of CYP6G1 to confer resistance to multiple insecticides exists in D. melanogaster and D. simulans but not in Drosophila willistoni or Drosophila virilis. In D. virilis, which has multiple copies of Cyp6g1, one copy confers resistance to DDT and another to nitenpyram, suggesting that the divergence of protein sequence between copies subsequent to the duplication affected the activity of the enzyme. All orthologs tested conferred resistance to one or more insecticides, suggesting that CYP6G1 had the capacity to provide resistance to anthropogenic chemicals before they existed. Finally, we show that expression of Cyp6g1 in the Malpighian tubules, which contributes to DDT resistance in D. melanogaster, is specific to the D. melanogaster-D. simulans lineage. Our results suggest that a combination of gene duplication, regulatory changes and protein coding changes has taken place at the Cyp6g1 locus during evolution and this locus may play a role in providing resistance to different environmental toxins in different Drosophila species.

  4. Alkaline "Permanent" Paper.

    Science.gov (United States)

    Pacey, Antony

    1991-01-01

    Discussion of paper manufacturing processes and their effects on library materials focuses on the promotion of alkaline "permanent" paper, with less acid, by Canadian library preservation specialists. Standards for paper acidity are explained; advantages of alkaline paper are described, including decreased manufacturing costs; and recyclability is…

  5. Brucine, an effective natural compound derived from nux-vomica, induces G1 phase arrest and apoptosis in LoVo cells.

    Science.gov (United States)

    Zheng, Lei; Wang, Xiaoli; Luo, Wenjuan; Zhan, Yingzhuan; Zhang, Yanmin

    2013-08-01

    Brucine is an alkaloid from nux vomica, has been shown various pharmacological actions. To study the possible anti-cancer mechanisms on LoVo cells, effects of Brucine on cell viability, cell cycle and apoptosis were investigated. The results showed that Brucine revealed strong growth inhibitory effect on LoVo cells, and caused LoVo cell shrinkage and membrane blobbing, induced cellular and DNA morphological changes. Cell cycle and apoptosis analysis documented that Brucine could change cell cycle and induce cell apoptosis. Brucine-mediated cell cycle arrest in G1 phase was associated with a marked increase of protein levels of CCND1 and decrease in CCNB1, cyclin E and CDC2. In addition, Brucine dose-dependently caused LoVo cells apoptosis evidenced by Annexin V/PI staining Brucine-induced apoptosis was mediated via up-regulation of Bax and down-regulation of Bcl-2. Furthermore, proteins Erk1/2, p38 and Akt phosphorylation were down regulated by Brucine in a dose-dependent manner. In summary, this paper indicates Brucine is effective against LoVo cells proliferation, and promotes LoVo cells death via apoptosis. These results reveal functional interplay among a series of pathway that are deregulated in cancer and suggest that their simultaneous targeting by Brucine could result in efficacious inhibition on cancer cells.

  6. p16INK4a, but not constitutively active pRb, can impose a sustained G1 arrest: molecular mechanisms and implications for oncogenesis

    DEFF Research Database (Denmark)

    Lukas, J; Sørensen, Claus Storgaard; Lukas, C;

    1999-01-01

    p16ink4 and pRb, two components of a key G1/S regulatory pathway, and tumor suppressors commonly targeted in oncogenesis, are among the candidates for gene therapy of cancer. Wild-type p16 and a constitutively active pRb(delta cdk) mutant both blocked G1 in short-term experiments, but only p16......)-expressing cells, became rapidly inhibited through restructuring diverse cyclin/CDK/p21 complexes by p16. These results provide novel insights into the roles of p16, pRb and cyclin E in G1/S control and multistep oncogenesis, with implications for gene therapy strategies....

  7. Suppression of polyclonal and antigen-specific murine IgG1 but not IgE responses by neutralizing interleukin-6 in vivo

    OpenAIRE

    Ommen, R.; Vredendaal, Astrid; Savelkoul, Huub

    1994-01-01

    textabstractThe crucial role of interleukin (IL)-4 in the induction of murine IgG1 and IgE responses, which are coupled through the process of sequential isotype switching, has been well documented. Whereas IL-4 is obligatory for the induction of IgE responses, it enhances IgG1 responses. In this study, using neutralizing antibodies, we provide evidence that, besides IL-4, also IL-6 is required for obtaining peak IgG1 responses. The mRNA levels of these two cytokines are coordinately expresse...

  8. Squamocin modulates histone H3 phosphorylation levels and induces G1 phase arrest and apoptosis in cancer cells

    Directory of Open Access Journals (Sweden)

    Wu Yang-Chang

    2011-02-01

    Full Text Available Abstract Background Histone modifications in tumorigenesis are increasingly recognized as important epigenetic factors leading to cancer. Increased phosphorylation levels of histone H3 as a result of aurora B and pMSK1 overexpression were observed in various tumors. We selected aurora B and MSK1 as representatives for testing various compounds and drugs, and found that squamocin, a bis-tetrahydrofuran annonaceous acetogenin, exerted a potent effect on histone H3 phosphorylation. Methods GBM8401, Huh-7, and SW620 cells were incubated with 15, 30, and 60 μM squamocin for 24 h. The expressions of mRNA and proteins were analyzed by qRT-PCR and Western blotting, respectively. The cell viability was determined by an MTT assay. Cell cycle distribution and apoptotic cells were analyzed by flow cytometry. Results Our results showed that squamocin inhibited the proliferation of GBM8401, Huh-7, and SW620 cells, arrested the cell cycle at the G1 phase, and activated both intrinsic and extrinsic pathways to apoptosis. In addition, we demonstrated that squamocin had the ability to modulate the phosphorylation levels of H3S10 (H3S10p and H3S28 (H3S28p in association with the downregulation of aurora B and pMSK1 expressions. Conclusions This study is the first to show that squamocin affects epigenetic alterations by modulating histone H3 phosphorylation at S10 and S28, providing a novel view of the antitumor mechanism of squamocin.

  9. UVB-induced cell death signaling is associated with G1-S progression and transcription inhibition in primary human fibroblasts.

    Directory of Open Access Journals (Sweden)

    Tatiana Grohmann Ortolan

    Full Text Available DNA damage induced by ultraviolet (UV radiation can be removed by nucleotide excision repair through two sub-pathways, one general (GGR and the other specific for transcribed DNA (TCR, and the processing of unrepaired lesions trigger signals that may lead to cell death. These signals involve the tumor suppressor p53 protein, a central regulator of cell responses to DNA damage, and the E3 ubiquitin ligase Mdm2, that forms a feedback regulatory loop with p53. The involvement of cell cycle and transcription on the signaling to apoptosis was investigated in UVB-irradiated synchronized, DNA repair proficient, CS-B (TCR-deficient and XP-C (GGR-deficient primary human fibroblasts. Cells were irradiated in the G1 phase of the cell cycle, with two doses with equivalent levels of apoptosis (low and high, defined for each cell line. In the three cell lines, the low doses of UVB caused only a transient delay in progression to the S phase, whereas the high doses induced permanent cell cycle arrest. However, while accumulation of Mdm2 correlated well with the recovery from transcription inhibition at the low doses for normal and CS-B fibroblasts, for XP-C cells this protein was shown to be accumulated even at UVB doses that induced high levels of apoptosis. Thus, UVB-induced accumulation of Mdm2 is critical for counteracting p53 activation and apoptosis avoidance, but its effect is limited due to transcription inhibition. However, in the case of XP-C cells, an excess of unrepaired DNA damage would be sufficient to block S phase progression, which would signal to apoptosis, independent of Mdm2 accumulation. The data clearly discriminate DNA damage signals that lead to cell death, depending on the presence of UVB-induced DNA damage in replicating or transcribing regions.

  10. Pristimerin causes G1 arrest, induces apoptosis, and enhances the chemosensitivity to gemcitabine in pancreatic cancer cells.

    Directory of Open Access Journals (Sweden)

    Yongwei Wang

    Full Text Available Despite rapid advances in chemotherapy and surgical resection strategies, pancreatic cancer remains the fourth leading cause of cancer related deaths in the United States with a 5-year survival rate of less than 5%. Therefore, novel therapeutic agents for the prevention and treatment of pancreatic cancer are urgently needed. The aim of this study was to investigate the effect of pristimerin, a quinonemethide triterpenoid compound isolated from Celastraceae and Hippocrateaceae, on inhibition of cell proliferation and induction of apoptosis in three pancreatic cancer cells, BxPC-3, PANC-1 and AsPC-1, in both monotherapy and in combination with gemcitabine. Treatment with pristimerin decreased the cell proliferation of all three pancreatic cancer cells in a dose- and time-dependent manner. Treatment of pancreatic cancer cells with pristimerin also resulted in G1-phase arrest which was strongly associated with a marked decrease in the level of cyclins (D1 and E and cyclin-dependent kinases (cdk2, cdk4 and cdk6 with concomitant induction of WAF1/p21 and KIP1/p27. Pristimerin treatment also resulted in apoptotic cell death, cleavage of caspase-3, modulation in the expressions of Bcl-2 family proteins, inhibition of the translocation and DNA-binding activity of NF-κB. In addition, pristimerin potentiated the growth inhibition and apoptosis inducing effects of gemcitabine in all three pancreatic cancer cells, at least in part, by inhibiting constitutive as well as gemcitabine-induced activation of NF-κB in both its DNA-binding activity and transcriptional activity. Taken together, these data provide the first evidence that pristimerin has strong potential for development as a novel agent against pancreatic cancer.

  11. PKCeta enhances cell cycle progression, the expression of G1 cyclins and p21 in MCF-7 cells.

    Science.gov (United States)

    Fima, E; Shtutman, M; Libros, P; Missel, A; Shahaf, G; Kahana, G; Livneh, E

    2001-10-11

    Protein kinase C encodes a family of enzymes implicated in cellular differentiation, growth control and tumor promotion. However, not much is known with respect to the molecular mechanisms that link protein kinase C to cell cycle control. Here we report that the expression of PKCeta in MCF-7 cells, under the control of a tetracycline-responsive inducible promoter, enhanced cell growth and affected the cell cycle at several points. The induced expression of another PKC isoform, PKCdelta, in MCF-7 cells had opposite effects and inhibited their growth. PKCeta expression activated cellular pathways in these cells that resulted in the increased expression of the G1 phase cyclins, cyclin D and cyclin E. Expression of the cyclin-dependent kinase inhibitor p21(WAF1) was also specifically elevated in PKCeta expressing cells, but its overall effects were not inhibitory. Although, the protein levels of the cyclin-dependent kinase inhibitor p27(KIP1) were not altered by the induced expression of PKCeta, the cyclin E associated Cdk2 kinase activity was in correlation with the p27(KIP1) bound to the cyclin E complex and not by p21(WAF1) binding. PKCeta expression enhanced the removal of p27(KIP1) from this complex, and its re-association with the cyclin D/Cdk4 complex. Reduced binding of p27(KIP1) to the cyclin D/Cdk4 complex at early time points of the cell cycle also enhanced the activity of this complex, while at later time points the decrease in bound p21(WAF1) correlated with its increased activity in PKCeta-expressing cells. Thus, PKCeta induces altered expression of several cell cycle functions, which may contribute to its ability to affect cell growth.

  12. New analytical techniques for mycotoxins in complex organic matrices. [Aflatoxins B1, B2, G1, and G2

    Energy Technology Data Exchange (ETDEWEB)

    Bicking, M.K.L.

    1982-07-01

    Air samples are collected for analysis from the Ames Solid Waste Recovery System. The high level of airborne fungi within the processing area is of concern due to the possible presence of toxic mycotoxins, and carcinogenic fungal metabolites. An analytical method has been developed to determine the concentration of aflatoxins B1, B2, G1, and G2 in the air of the plant which produces Refuse Derived Fuel (RDF). After extraction with methanol, some components in the matrix are precipitated by dissolving the sample in 30% acetonitrile/chloroform. An aliquot of this solution is injected onto a Styragel column where the sample components undergo simultaneous size exclusion and reverse phase partitioning. Additional studies have provided a more thorough understanding of solvent related non-exclusion effects on size exclusion gels. The Styragel column appears to have a useable lifetime of more than six months. After elution from Styragel, the sample is diverted to a second column containing Florisil which has been modified with oxalic acid and deactivated with water. Aflatoxins are eluted with 5% water/acetone. After removal of this solvent, the sample is dissolved in 150 ..mu..L of a spotting solvent and the entire sample applied to a thin layer chromatography (TLC) plate using a unique sample applicator developed here. The aflatoxins on the TLC plate are analyzed by laser fluorescence. A detection limit of 10 pg is possible for aflatoxin standards using a nitrogen laser as the excitation source. Sample concentrations are determined by comparing with an internal standard, a specially synthesized aflatoxin derivative. In two separate RDF samples, aflatoxin B1 was found at levels of 6.5 and 17.0 ppB. The analytical method has also proven useful in the analysis of contaminated corn and peanut meal samples. 42 figures, 8 tables.

  13. 3,3'-Diindolylmethane induces G1 arrest and apoptosis in human acute T-cell lymphoblastic leukemia cells.

    Directory of Open Access Journals (Sweden)

    Lyndsey E Shorey

    Full Text Available Certain bioactive food components, including indole-3-carbinol (I3C and 3,3'-diindolylmethane (DIM from cruciferous vegetables, have been shown to target cellular pathways regulating carcinogenesis. Previously, our laboratory showed that dietary I3C is an effective transplacental chemopreventive agent in a dibenzo[def,p]chrysene (DBC-dependent model of murine T-cell lymphoblastic lymphoma. The primary objective of the present study was to extend our chemoprevention studies in mice to an analogous human neoplasm in cell culture. Therefore, we tested the hypothesis that I3C or DIM may be chemotherapeutic in human T-cell acute lymphoblastic leukemia (T-ALL cells. Treatment of the T-ALL cell lines CCRF-CEM, CCRF-HSB2, SUP-T1 and Jurkat with DIM in vitro significantly reduced cell proliferation and viability at concentrations 8- to 25-fold lower than the parent compound I3C. DIM (7.5 µM arrested CEM and HSB2 cells at the G(1 phase of the cell cycle and 15 µM DIM significantly increased the percentage of apoptotic cells in all T-ALL lines. In CEM cells, DIM reduced protein expression of cyclin dependent kinases 4 and 6 (CDK4, CDK6 and D-type cyclin 3 (CCND3; DIM also significantly altered expression of eight transcripts related to human apoptosis (BCL2L10, CD40LG, HRK, TNF, TNFRSF1A, TNFRSF25, TNFSF8, TRAF4. Similar anticancer effects of DIM were observed in vivo. Dietary exposure to 100 ppm DIM significantly decreased the rate of growth of human CEM xenografts in immunodeficient SCID mice, reduced final tumor size by 44% and increased the apoptotic index compared to control-fed mice. Taken together, our results demonstrate a potential for therapeutic application of DIM in T-ALL.

  14. A haploid genetic screen identifies the G1/S regulatory machinery as a determinant of Wee1 inhibitor sensitivity.

    Science.gov (United States)

    Heijink, Anne Margriet; Blomen, Vincent A; Bisteau, Xavier; Degener, Fabian; Matsushita, Felipe Yu; Kaldis, Philipp; Foijer, Floris; van Vugt, Marcel A T M

    2015-12-01

    The Wee1 cell cycle checkpoint kinase prevents premature mitotic entry by inhibiting cyclin-dependent kinases. Chemical inhibitors of Wee1 are currently being tested clinically as targeted anticancer drugs. Wee1 inhibition is thought to be preferentially cytotoxic in p53-defective cancer cells. However, TP53 mutant cancers do not respond consistently to Wee1 inhibitor treatment, indicating the existence of genetic determinants of Wee1 inhibitor sensitivity other than TP53 status. To optimally facilitate patient selection for Wee1 inhibition and uncover potential resistance mechanisms, identification of these currently unknown genes is necessary. The aim of this study was therefore to identify gene mutations that determine Wee1 inhibitor sensitivity. We performed a genome-wide unbiased functional genetic screen in TP53 mutant near-haploid KBM-7 cells using gene-trap insertional mutagenesis. Insertion site mapping of cells that survived long-term Wee1 inhibition revealed enrichment of G1/S regulatory genes, including SKP2, CUL1, and CDK2. Stable depletion of SKP2, CUL1, or CDK2 or chemical Cdk2 inhibition rescued the γ-H2AX induction and abrogation of G2 phase as induced by Wee1 inhibition in breast and ovarian cancer cell lines. Remarkably, live cell imaging showed that depletion of SKP2, CUL1, or CDK2 did not rescue the Wee1 inhibition-induced karyokinesis and cytokinesis defects. These data indicate that the activity of the DNA replication machinery, beyond TP53 mutation status, determines Wee1 inhibitor sensitivity, and could serve as a selection criterion for Wee1-inhibitor eligible patients. Conversely, loss of the identified S-phase genes could serve as a mechanism of acquired resistance, which goes along with development of severe genomic instability. PMID:26598692

  15. Stratigraphy and structure of volcanic rocks in drill hole USW-G1, Yucca Mountain, Nye County, Nevada

    International Nuclear Information System (INIS)

    Detailed subsurface studies in connection with the Nevada Nuclear Waste Storage Investigations program are being conducted to investigate the stratigraphic and structural features of volcanic rocks underlying Yucca Mountain, a volcanic highland situated along the western boundary of the Nevada Test Site in southern Nevada. As part of this continuing effort, drill hole USW-G1 was cored from 292 ft to a depth of 6000 ft from March to August 1980. The stratigraphic section is composed of thick sequences of ash-flow tuff and volcanic breccia interbedded with subordinate amounts of fine- to coarse-grained volcaniclastic rocks. All rocks are of Tertiary age and vary in composition from rhyolite to dacite. The 3005-ft level in the drill hole represents a significant demarcation between unaltered and altered volcanic rocks. For the most part, tuff units above 3005 ft appear devitrified and show little secondary alteration except within tuffaceous beds of Calico Hills, where the rock contains 60 to 80% zeolites. Below 3005 ft, most rocks show intermittent to pervasive alteration to clay minerals and zeolites. Examination of core for structural features revealed the presence of 61 shear fractures, 528 joints, and 4 conspicuous fault zones. Shear fractures mainly occurred in the Topopah Spring Member of the Paintbrush Tuff, flow breccia, and near fault zones. Nearly 88% of shear and joint surfaces show evidence of coatings. Approximately 40% of the fractures were categorized as completely healed. Rock quality characteristics as defined by the core index indicate that greater amounts of broken and lost core are commonly associated with (1) the densely welded zone of the Topopah Spring, (2) highly silicified zones, and (3) fault zones

  16. Villous adenoma of the distal appendix.

    Science.gov (United States)

    Taylor, J V; Thomas, M G; Kelly, S; Sutton, R

    1997-04-01

    Villous adenoma confined to the distal appendix has not been previously reported in conjunction with acute apendicitis. The presence of an adenoma indicates a need for further investigation due to an association with neoplasia elsewhere.

  17. Russian Geologic Repository Technical Papers and Reports

    Energy Technology Data Exchange (ETDEWEB)

    Jardine, L

    2002-02-18

    developing various aspects of geologic repository sites in Russia. Most, but not all, of these have been prepared by LLNL staff, by Russian experts in collaboration with LLNL staff, or by Russian organizations under an LLNL contract using DOE funds. The purpose of this report is to put into one LLNL document the collection of current papers and reports in order to summarize and document the status of the Russian geologic repository programs for which LLNL has been directly and continuously involved since 1995. This report will be useful to many interested in the RF geologic disposal activities. The collection demonstrates the seriousness and depth of the RF institutes and LLNL activities in recent years to develop a RF geologic repository program, even with very limited funds. This report is organized into five sections. After the Introduction, Section II provides a listing of technical papers and reports involving RF geologic repository topics of an overview, policy, or planning nature. The next two sections, III and IV, provide a listing of technical papers and reports for the specific sites near K-26 and Mayak, respectively. A brief summary and background is provided in Sections II, III, and IV preceding the list of papers and reports that pertain to each section. Finally, Section V provides some final comments regarding the LLNL perspective of the RF geologic disposal activities and prospects. The appendices to this document contain the full text of all the RF geologic disposal papers and reports listed in Sections II-IV. The documents in Appendix A are overview, policy, and planning documents. Appendix B contains the K-26 site-specific documents, and Appendix C contains the Mayak site-specific documents.

  18. Production, Characterization, and Biological Evaluation of Well-Defined IgG1 Fc Glycoforms as a Model System for Biosimilarity Analysis.

    Science.gov (United States)

    Okbazghi, Solomon Z; More, Apurva S; White, Derek R; Duan, Shaofeng; Shah, Ishan S; Joshi, Sangeeta B; Middaugh, C Russell; Volkin, David B; Tolbert, Thomas J

    2016-02-01

    Four different well-defined IgG1 Fc glycoforms are proposed as a model system to examine important biological and physicochemical features for protein drug biosimilar analyses. The IgG1 Fc glycoforms were produced by yeast expression combined with in vitro enzymatic synthesis as a series of sequentially truncated high-mannose IgG1 Fc glycoforms with an anticipated range of biological activity and structural stability. Initial characterization with mass spectrometry, SDS-PAGE, size exclusion HPLC, and capillary isoelectric focusing confirmed that the glycoproteins are overall highly similar with the only major difference being glycosylation state. Binding to the activating Fc receptor, FcγRIIIa was used to evaluate the potential biological activity of the IgG1 Fc glycoproteins. Two complementary methods using biolayer interferometry, 1 with protein G-immobilized IgG1 Fc and the other with streptavidin-immobilized FcγRIIIa, were developed to assess FcγRIIIa affinity in kinetic binding studies. The high-mannose IgG1 Fc and Man5-IgG1 Fc glycoforms were highly similar to one another with high affinity for FcγRIIIa, whereas GlcNAc-Fc had weak affinity, and the nonglycosylated N297Q-Fc had no measurable affinity for FcγRIIIa. These 4 IgG1 Fc glycoforms were also evaluated in terms of physical and chemical stability profiles and then used as a model system to mathematically assess overall biosimilarity, as described in a series of companion articles. PMID:26869419

  19. G1-4A, a Polysaccharide from Tinospora cordifolia Inhibits the Survival of Mycobacterium tuberculosis by Modulating Host Immune Responses in TLR4 Dependent Manner

    OpenAIRE

    Pramod Kumar Gupta; Pampi Chakraborty; Santosh Kumar; Prafull Kumar Singh; Rajan, M. G. R.; Krishna B Sainis; Savita Kulkarni

    2016-01-01

    Rapid emergence of drug resistance in Mycobacterium tuberculosis (MTB) is a major health concern and demands the development of novel adjunct immunotherapeutic agents capable of modulating the host immune responses in order to control the pathogen. In the present study, we sought to investigate the immunomodulatory effects of G1-4A, a polysaccharide derived from the Indian medicinal plant Tinospora cordifolia, in in-vitro and aerosol mouse models of MTB infection. G1-4A treatment of MTB infec...

  20. Evolutionary Changes in Gene Expression, Coding Sequence and Copy-Number at the Cyp6g1 Locus Contribute to Resistance to Multiple Insecticides in Drosophila

    OpenAIRE

    Harrop, Thomas W. R.; Tamar Sztal; Christopher Lumb; Good, Robert T.; Daborn, Phillip J.; Philip Batterham; Henry Chung

    2014-01-01

    Widespread use of insecticides has led to insecticide resistance in many populations of insects. In some populations, resistance has evolved to multiple pesticides. In Drosophila melanogaster, resistance to multiple classes of insecticide is due to the overexpression of a single cytochrome P450 gene, Cyp6g1. Overexpression of Cyp6g1 appears to have evolved in parallel in Drosophila simulans, a sibling species of D. melanogaster, where it is also associated with insecticide resistance. However...

  1. Audio Papers - A Manifesto

    DEFF Research Database (Denmark)

    Krogh Groth, Sanne; Samson, Kristine

    2016-01-01

    Audio papers resemble the regular essay or the academic text in that they deal with a certain topic of interest, but presented in the form of an audio production. The audio paper is an extension of the written paper through its specific use of media, a sonic awareness of aesthetics and materialit...

  2. Nucleotide mismatches between the VP7 gene and the primer are associated with genotyping failure of a specific lineage from G1 rotavirus strains

    Directory of Open Access Journals (Sweden)

    Espinola Emilio E

    2006-05-01

    Full Text Available Abstract In recent years it was reported that the accumulation of point mutations in VP4 and VP7 genes of rotavirus strains was the main cause of the failure of the G or P-typing. Failures in the correct genotyping of G1, G2, G8, G9 and G10 rotavirus strains were reported in the most commonly used reverse transcription (RT-PCR strategies. Collecting VP7 gene sequences of G1 rotavirus strains from databases we found that 74 (61.2 % out of 121 G1 strains from lineage I showed the four specific mismatches at the 5' end of the 9T1-1 primer, previously associated with the failure of G1-typing. Thus, a great percentage of the G1 strains from lineage I worldwide reported could not have been typed if the Das's RT-PCR strategy were used. This analysis shows that the failure on the detection of the G1 strains could be due to the diversification of rotavirus strains in phylogenetic lineages. Therefore, the use of different RT-PCR strategies with different primer binding locations on the VP7 gene or new typing methodologies -like microarrays procedures- could be a better option to avoid the failure of the G-typing of rotavirus strains detected during surveillance programs.

  3. G1-4A, a polysaccharide from Tinospora cordifolia induces peroxynitrite dependent killer dendritic cell (KDC) activity against tumor cells.

    Science.gov (United States)

    Pandey, Vipul K; Amin, Prayag J; Shankar, Bhavani S

    2014-12-01

    Dendritic cells (DC) play a central role in the development of an adaptive immune response against tumor. In addition to its role in antigen presentation, DC also possesses cytotoxic activity against tumor cells. We have earlier shown phenotypic and functional maturation of bone marrow derived dendritic cells (BMDC) by G1-4A, an arabinogalactan derived from Tinospora cordifolia. In this study, we have investigated the killer phenotype of BMDC matured in the presence of G1-4A, [mBMDC (G1-4A)] on tumor cells. We have observed several fold increase in killing of tumor cells by mBMDC (G1-4A). The tumoricidal activity was not specific to syngeneic tumors cells but could kill xenogenic tumors also. Nitric oxide released by mBMDC (G1-4A) generates peroxynitrite in tumor cells and is responsible for killing of target cells. This killing was completely abrogated by inducible nitric oxide synthase (iNOS) inhibitor 1400W and NADPH oxidase inhibitor apocyanin. The killed target cells are phagocytosed by BMDC which further activate syngeneic cytotoxic T cells. These results thus show that G1-4A treated mBMDC acquire killer phenotype along with maturation which plays an important role in activation of cytotoxic T cells. PMID:25278461

  4. A Discrete-Time Geo/G/1 Retrial Queue with Second Optional Vacation and General Retrial Times%带有二次可选休假和一般重试时间的Geo/G/1重试排队

    Institute of Scientific and Technical Information of China (English)

    蔡梨; 韦才敏; 覃毅延

    2014-01-01

    Discrete time queues are more appropriate to modeling slotted systems than continuous time queues,such as computer and communication systems. In this paper,a discrete-time Geo/G/1 retrial queue with second optional vacation where the retrial times follow a general distribution was studied. The probability generating functions of the orbit size,system size and stochastic decomposition property in steady-state were studied using supplementary variable technique and probability generating functions method. In addition, this model is verified by special examples. Finally, numerical examples are presented to illustrate the effects of different parameters on the system performances.%离散时间排队比连续时间排队在有时间间隔的系统中更适合建立模型,例如计算机和通讯系统。考虑一个具有二次可选休假和一般重试时间的Geo/G/1离散重试排队模型。采用补充变量法和构造母函数等方法研究该系统在稳态条件下轨道顾客数、系统顾客数的概率母函数和随机分解等性质,通过模型的特例来验证模型的正确性。最后给出一些数值例子分析不同的参数对系统性能指标的影响。

  5. Queue length distribution and numerical calculation for Geo/G/1 queueing system with delayed N-policy%延迟N-策略Geo/G/1排队系统的队长分布及数值计算

    Institute of Scientific and Technical Information of China (English)

    魏瑛源; 唐应辉; 顾建雄

    2011-01-01

    This paper considers the discrete-time Geo/G/1 queueing system with delayed iV-policy. By using the total probability decomposition technique, we study the transient and equilibrium properties of the queue length from the beginning of the any initial state, obtain both the recursion expressions of the z-transformation of the transient queue length distribution at any time n and the recursion expressions of the steady state queue length distribution, and the stochastic decomposition of the queue length at a random point in equilibrium. Finally, by numerical examples, we discuss the sensitivity of the steady state queue length distribution towards system parameters, and illustrate the important value of the expressions of the steady state queue length distribution for calculating conveniently in the system capacity design.%考虑延迟N-策略离散时间Geo/G/1排队系统,使用全概率分解技术,从任意初始状态出发,研究了队长的瞬态和稳态性质,导出了在任意时刻n瞬态队长分布的z-变换的递推表达式和稳态队长分布的递推表达式,以及稳态队长的随机分解.最后,通过数值实例,讨论了稳态队长分布对系统参数的敏感性,并阐述了获得便于计算的稳态队长分布的表达式在系统容量设计中有重要的价值.

  6. 40 CFR Appendix A to Subpart A of... - Appendix A to Subpart A of Part 750

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Appendix A to Subpart A of Part 750 A Appendix A to Subpart A of Part 750 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... ACT Procedures for Rulemaking Under Section 6 of the Toxic Substances Control Act Pt. 750, Subpt....

  7. 40 CFR Appendix I to Subpart B of... - Appendix I to Subpart B of Part 205

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Appendix I to Subpart B of Part 205 I Appendix I to Subpart B of Part 205 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS Medium and Heavy Trucks...

  8. 13 CFR Appendix A to Part 112 - Appendix A to Part 112

    Science.gov (United States)

    2010-01-01

    ... development centers Small Business Act, sec. 21 and Pub. L. 96-302. International Trade Program Small Business... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Appendix A to Part 112 A Appendix A to Part 112 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NONDISCRIMINATION...

  9. 13 CFR Appendix A to Part 1171 - Appendix A to Part 1171

    Science.gov (United States)

    2010-01-01

    .... International Trade Program Small Business Act, section 22. Technical and Management Assistance Small Business... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Appendix A to Part 1171 A Appendix A to Part 1171 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NONDISCRIMINATION...

  10. Aerosol concentration and size distribution measured below, in, and above cloud from the DOE G-1 during VOCALS-REx

    Science.gov (United States)

    Kleinman, L. I.; Daum, P. H.; Lee, Y.-N.; Lewis, E. R.; Sedlacek, A. J., III; Senum, G. I.; Springston, S. R.; Wang, J.; Hubbe, J.; Jayne, J.; Min, Q.; Yum, S. S.; Allen, G.

    2011-06-01

    During the VOCALS Regional Experiment, the DOE G-1 aircraft was used to sample a varying aerosol environment pertinent to properties of stratocumulus clouds over a longitude band extending 800 km west from the Chilean coast at Arica. Trace gas and aerosol measurements are presented as a function of longitude, altitude, and dew point in this study. Spatial distributions are consistent with an upper atmospheric source for O3 and South American coastal sources for marine boundary layer (MBL) CO and aerosol, most of which is acidic sulfate in agreement with the dominant pollution source being SO2 from Cu smelters and power plants. Pollutant layers in the free troposphere (FT) can be a result of emissions to the north in Peru or long range transport from the west. At a given altitude in the FT (up to 3 km), dew point varies by 40 °C with dry air descending from the upper atmospheric and moist air having a BL contribution. Ascent of BL air to a cold high altitude results in the condensation and precipitation removal of all but a few percent of BL water along with aerosol that served as CCN. Thus, aerosol volume decreases with dew point in the FT. Aerosol size spectra have a bimodal structure in the MBL and an intermediate diameter unimodal distribution in the FT. Comparing cloud droplet number concentration (CDNC) and pre-cloud aerosol (Dp > 100 nm) gives a linear relation up to a number concentration of ~150 cm-3, followed by a less than proportional increase in CDNC at higher aerosol number concentration. A number balance between below cloud aerosol and cloud droplets indicates that ~25 % of aerosol in the PCASP size range are interstitial (not activated). One hundred and two constant altitude cloud transects were identified and used to determine properties of interstitial aerosol. One transect is examined in detail as a case study. Approximately 25 to 50 % of aerosol with Dp > 110 nm were not activated, the difference between the two approaches possibly representing

  11. Oleanolic acid induces mitochondrial-dependent apoptosis and G0/G1 phase arrest in gallbladder cancer cells

    Directory of Open Access Journals (Sweden)

    Li HF

    2015-06-01

    Full Text Available Huai-Feng Li,1–3,* Xu-An Wang,1–3,* Shan-Shan Xiang,1–3,* Yun-Ping Hu,1–3 Lin Jiang,1–3 Yi-Jun Shu,1–3 Mao-Lan Li,1–3 Xiang-Song Wu,1–3 Fei Zhang,1–3 Yuan-Yuan Ye,1–3 Hao Weng,1–3 Run-Fa Bao,1–3 Yang Cao,1–3 Wei Lu,1–3 Qian Dong,1–3 Ying-Bin Liu1–3 1Department of General Surgery, 2Laboratory of General Surgery, 3Institute of Biliary Tract Disease, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Oleanolic acid (OA, a naturally occurring triterpenoid, exhibits potential antitumor activity in many tumor cell lines. Gallbladder carcinoma is the most common malignancy of the biliary tract, and is a highly aggressive tumor with an extremely poor prognosis. Unfortunately, the effects of OA on gallbladder carcinoma are unknown. In this study, we investigated the effects of OA on gallbladder cancer cells and the underlying mechanism. The results showed that OA inhibits proliferation of gallbladder cancer cells in a dose-dependent and time-dependent manner on MTT and colony formation assay. A flow cytometry assay revealed apoptosis and G0/G1 phase arrest in GBC-SD and NOZ cells. Western blot analysis and a mitochondrial membrane potential assay demonstrated that OA functions through the mitochondrial apoptosis pathway. Moreover, this drug inhibited tumor growth in nude mice carrying subcutaneous NOZ tumor xenografts. These data suggest that OA inhibits proliferation of gallbladder cancer cells by regulating apoptosis and the cell cycle process. Thus, OA may be a promising drug for adjuvant chemotherapy in gallbladder carcinoma. Keywords: oleanolic acid, gallbladder carcinoma, apoptosis, cell cycle arrest, mitochondrial pathway

  12. Aerosol concentration and size distribution measured below, in, and above cloud from the DOE G-1 during VOCALS-REx

    Directory of Open Access Journals (Sweden)

    L. I. Kleinman

    2011-06-01

    Full Text Available During the VOCALS Regional Experiment, the DOE G-1 aircraft was used to sample a varying aerosol environment pertinent to properties of stratocumulus clouds over a longitude band extending 800 km west from the Chilean coast at Arica. Trace gas and aerosol measurements are presented as a function of longitude, altitude, and dew point in this study. Spatial distributions are consistent with an upper atmospheric source for O3 and South American coastal sources for marine boundary layer (MBL CO and aerosol, most of which is acidic sulfate in agreement with the dominant pollution source being SO2 from Cu smelters and power plants. Pollutant layers in the free troposphere (FT can be a result of emissions to the north in Peru or long range transport from the west. At a given altitude in the FT (up to 3 km, dew point varies by 40 °C with dry air descending from the upper atmospheric and moist air having a BL contribution. Ascent of BL air to a cold high altitude results in the condensation and precipitation removal of all but a few percent of BL water along with aerosol that served as CCN. Thus, aerosol volume decreases with dew point in the FT. Aerosol size spectra have a bimodal structure in the MBL and an intermediate diameter unimodal distribution in the FT. Comparing cloud droplet number concentration (CDNC and pre-cloud aerosol (Dp > 100 nm gives a linear relation up to a number concentration of ~150 cm−3, followed by a less than proportional increase in CDNC at higher aerosol number concentration. A number balance between below cloud aerosol and cloud droplets indicates that ~25 % of aerosol in the PCASP size range are interstitial (not activated. One hundred and two constant altitude cloud transects were identified and used to determine properties of interstitial aerosol. One transect is examined in detail as a case study. Approximately 25 to 50 % of aerosol with Dp > 110 nm

  13. THE HARD X-RAY VIEW OF THE YOUNG SUPERNOVA REMNANT G1.9+0.3

    Energy Technology Data Exchange (ETDEWEB)

    Zoglauer, Andreas; Boggs, Steven E.; Craig, William W.; Krivonos, Roman A. [Space Sciences Laboratory, University of California, Berkeley, CA 94720 (United States); Reynolds, Stephen P. [Physics Department, North Carolina State University, Raleigh, NC 27695 (United States); An, Hongjun [Department of Physics, McGill University, Montreal, Quebec, H3A 2T8 (Canada); Christensen, Finn E. [DTU Space, National Space Institute, Technical University of Denmark, Elektrovej 327, DK-2800 Lyngby (Denmark); Fryer, Chris L. [CCS-2, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Grefenstette, Brian W.; Harrison, Fiona A.; Madsen, Kristin K.; Miyasaka, Hiromasa [Cahill Center for Astronomy and Astrophysics, California Institute of Technology, Pasadena, CA 91125 (United States); Hailey, Charles J. [Columbia Astrophysics Laboratory, Columbia University, New York, NY 10027 (United States); Stern, Daniel [Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109 (United States); Zhang, William W., E-mail: zog@ssl.berkeley.edu [Goddard Space Flight Center, Greenbelt, MD 20771 (United States)

    2015-01-10

    NuSTAR observed G1.9+0.3, the youngest known supernova remnant in the Milky Way, for 350 ks and detected emission up to ∼30 keV. The remnant's X-ray morphology does not change significantly across the energy range from 3 to 20 keV. A combined fit between NuSTAR and Chandra shows that the spectrum steepens with energy. The spectral shape can be well fitted with synchrotron emission from a power-law electron energy distribution with an exponential cutoff with no additional features. It can also be described by a purely phenomenological model such as a broken power law or a power law with an exponential cutoff, though these descriptions lack physical motivation. Using a fixed radio flux at 1 GHz of 1.17 Jy for the synchrotron model, we get a column density of N {sub H} = (7.23 ± 0.07) × 10{sup 22} cm{sup –2}, a spectral index of α = 0.633 ± 0.003, and a roll-off frequency of ν{sub rolloff} = (3.07 ± 0.18) × 10{sup 17} Hz. This can be explained by particle acceleration, to a maximum energy set by the finite remnant age, in a magnetic field of about 10 μG, for which our roll-off implies a maximum energy of about 100 TeV for both electrons and ions. Much higher magnetic-field strengths would produce an electron spectrum that was cut off by radiative losses, giving a much higher roll-off frequency that is independent of magnetic-field strength. In this case, ions could be accelerated to much higher energies. A search for {sup 44}Ti emission in the 67.9 keV line results in an upper limit of 1.5 × 10{sup –5} photons cm{sup –2} s{sup –1} assuming a line width of 4.0 keV (1 sigma)

  14. The physics of paper

    Energy Technology Data Exchange (ETDEWEB)

    Alava, Mikko [Helsinki University of Technology, Laboratory of Physics, PO Box 1100, 02015 HUT (Finland); Niskanen, Kaarlo [KCL Science and Consulting, PO Box 70, 02150 Espoo (Finland)

    2006-03-01

    Paper is a material known to everybody. It has a network structure consisting of wood fibres that can be mimicked by cooking a portion of spaghetti and pouring it on a plate, to form a planar assembly of fibres that lie roughly horizontal. Real paper also contains other constituents added for technical purposes. This review has two main lines of thought. First, in the introductory part, we consider the physics that one encounters when 'using' paper, an everyday material that exhibits the presence of disorder. Questions arise, for instance, as to why some papers are opaque and others translucent, some are sturdy and others sloppy, some readily absorb drops of liquid while others resist the penetration of water. The mechanical and rheological properties of paper and paperboard are also interesting. They are inherently dependent on moisture content. In humid conditions paper is ductile and soft, in dry conditions brittle and hard. In the second part we explain in more detail research problems concerned with paper. We start with paper structure. Paper is made by dewatering a suspension of fibres starting from very low content of solids. The processes of aggregation, sedimentation and clustering are familiar from statistical mechanics. Statistical growth models or packing models can simulate paper formation well and teach a lot about its structure. The second research area that we consider is the elastic and viscoelastic properties and fracture of paper and paperboard. This has traditionally been the strongest area of paper physics. There are many similarities to, but also important differences from, composite materials. Paper has proved to be convenient test material for new theories in statistical fracture mechanics. Polymer physics and memory effects are encountered when studying creep and stress relaxation in paper. Water is a 'softener' of paper. In humid conditions, the creep rate of paper is much higher than in dry conditions. The third among

  15. Paper Machine Energy Fingerprint

    OpenAIRE

    Lindberg, Carl-Fredrik; Starr, Kevin; Horton, Robert; Bhuthani, Naveen

    2010-01-01

    There is a large value in making the Pulp & Paper mills more energy efficient. ABB has developed an energy assessment service where opportunities to save energy in the paper machine are identified. The assessment is done by both quantifying energy flows and benchmarking energy users in the paper machine. After the assessment is done an action plan is presented to the mill of what could be done to improve energy efficiency together with a return on investment.

  16. Malaysia; Background Paper

    OpenAIRE

    International Monetary Fund

    1996-01-01

    This Background Paper on Malaysia examines developments and trends in the labor market since the mid-1980s. The paper describes the changes in the employment structure and the labor force. It reviews wages and productivity trends and their effects on unit labor cost. The paper highlights that Malaysia’s rapid growth, sustained since 1987, has had a major impact on the labor market. The paper outlines the major policy measures to address the labor constraints. It also analyzes Malaysia’s r...

  17. How wet paper curls

    Science.gov (United States)

    Reyssat, E.; Mahadevan, L.

    2011-03-01

    When a piece of tracing paper is placed gently on the surface of a bath of water, it rapidly curls up from one edge and rolls up due to the swelling of the side in contact with water. With time, as the swelling front propagates through the thickness of the paper, the paper gradually uncurls itself and eventually straightens out. We analyze the experimental dynamics of rolling and unrolling of the paper and complement these with a minimal theory that explains the basic observations. Our study might be useful in the context of designing biomimetic devices that work as actuators or harness energy from humidity variations.

  18. Paper or Gold

    OpenAIRE

    Mukund Raj

    2003-01-01

    In our society today, money's value is measured by what it can buy—its purchasing power—not by its material worth, but it hasn't always been so. · My previous papers Impact of agriculture output on exchange rates and Currency competition-Survival of the fittest dealt with issues surrounding exchange rate and currency competitions. This paper- Paper or Gold discusses the validity of human society giving importance to paper money. · We all know that the human race always believes in experimenti...

  19. High concentrations of therapeutic IgG1 antibodies are needed to compensate for inhibition of antibody-dependent cellular cytotoxicity by excess endogenous immunoglobulin G.

    Science.gov (United States)

    Preithner, Susanne; Elm, Stefanie; Lippold, Sandra; Locher, Mathias; Wolf, Andreas; da Silva, Antonio J; Baeuerle, Patrick A; Prang, Nadja S

    2006-03-01

    A common feature of human IgG1 antibodies used for cancer treatment is that their anti-tumour efficacy requires high serum trough levels and continued therapy for several months. Treatment cycles, thereby, consume several grams of IgG1 translating into significant drug needs and costs. The basis for the low in vivo efficacy, which is in contrast to high in vitro antibody-dependent cellular cytotoxicity (ADCC), is not well understood. Here, we have explored factors contributing to this discrepancy using adecatumumab (MT201), a fully human monoclonal IgG1 against epithelial cell adhesion molecule (Ep-CAM) and trastuzumab (Herceptin), a humanized IgG1 with specificity for the human epithelial growth factor receptor type 2 (HER-2) antigen. We found that physiological levels of human sera strongly inhibited ADCC of both IgG1 antibodies. Effects showed some dependence on the density of Ep-CAM and HER-2 targets, the tumour cell line tested and on effector cell and serum donors. Removal of IgG by affinity chromatography abolished the inhibitory effect of a serum pool. Inhibition of ADCC was fully restored by adding back the IgG fraction or by an equal amount of IgG from a commercial source. We further demonstrate that CD56-positive lymphocytes within human PBMC contributed >90% to ADCC and that normal serum levels of IgG effectively competed for in vitro binding of an IgG1 antibody to low-affinity Fcgamma receptor type III (CD16), as is present on natural killer (NK) cells. Competition of serum IgG for binding of therapeutic IgG1 to NK cell may be one important reason why high antibody doses are required in the clinic for treatment of cancer by an ADCC-based mechanism. PMID:16102830

  20. Inactivation of normal human fibroblasts by ionising irradiation results to a similar extent from chromosomal damage and p53-dependent G1-arrest

    International Nuclear Information System (INIS)

    After ionizing irradiation, fibroblasts lose clonogenicity (1) by non-repaired DNA double-strand breaks leading to lethal chromosome aberrations and (2) by permanent G1 arrest. The aim of this study was to determine the relative contribution of these two processes. 13 normal human fibroblast strains and 3 cell lines with non-functional p53 (LFS2800, FaDu, CHO). Cells were irradiated in plateau phase followed by immediate or delayed (14 h) plating. Lethal chromosome aberrations (CA) were measured by metaphase technique, the fraction of cells permanently arrested in G1 (fG1arr) by flow cytometry and cell survival by colony assay. For normal human fibroblasts, the number of lethal chromosome aberrations increased with dose but varied substantially among the strains studied. Only for delayed but not immediate plating the surviving fraction was correlated with the number of lethal aberrations (r2 =0.69, p2 =0.19, p=0.16). When survival was converted into lethal events the ratio between these events and the number of lethal aberrations amounted to 2.00±0.05:1, indicating that chromosomal damage accounted on average for only 50% of cell killing. The remainder was attributed to cell inactivation by the p53-dependent permanent G1-arrest, since cells lacking in functional p53 (LFS2800, FaDu, CHO) were characterised by a ratio of 1.01±0.02:1. In addition, there was a negative correlation between the extent of G1-arrest and the number of CA with those cell lines showing the highest G1-arrest having the lowest number of CA indicating that there is an interaction between these two processes. For normal human fibroblasts, cell inactivation results from chromosomal damage and permanent G1 arrest to a similar extent

  1. G1-4A, a Polysaccharide from Tinospora cordifolia Inhibits the Survival of Mycobacterium tuberculosis by Modulating Host Immune Responses in TLR4 Dependent Manner.

    Directory of Open Access Journals (Sweden)

    Pramod Kumar Gupta

    Full Text Available Rapid emergence of drug resistance in Mycobacterium tuberculosis (MTB is a major health concern and demands the development of novel adjunct immunotherapeutic agents capable of modulating the host immune responses in order to control the pathogen. In the present study, we sought to investigate the immunomodulatory effects of G1-4A, a polysaccharide derived from the Indian medicinal plant Tinospora cordifolia, in in-vitro and aerosol mouse models of MTB infection. G1-4A treatment of MTB infected RAW264.7 macrophages significantly induced the surface expression of MHC-II and CD-86 molecules, secretion of proinflammatory cytokines (TNF-α, IL-β, IL-6, IL-12, IFN-γ and nitric oxide leading to reduced intracellular survival of both drug sensitive (H37Rv as well as multi drug resistant strains (Beijing and LAM of MTB, which was partially attributed to G1-4A induced NO production in TLR4-MyD88 dependent manner. Similarly, bacillary burden was significantly reduced in the lungs of MTB infected BALB/c mice treated with G1-4A, with simultaneous up-regulation of the expression of TNF-α, INF-γ and NOS2 in the mouse lung along with increased levels of Th1 cytokines like IFN-γ, IL-12 and decreased levels of Th2 cytokine like IL-4 in the serum. Furthermore, combination of G1-4A with Isoniazid (INH exhibited better protection against MTB compared to that due to INH or G1-4A alone, suggesting its potential as adjunct therapy. Our results demonstrate that modulation of host immune responses by G1-4A might improve the therapeutic efficacy of existing anti-tubercular drugs and provide an attractive strategy for the development of alternative therapies to control tuberculosis.

  2. G1-4A, a Polysaccharide from Tinospora cordifolia Inhibits the Survival of Mycobacterium tuberculosis by Modulating Host Immune Responses in TLR4 Dependent Manner.

    Science.gov (United States)

    Gupta, Pramod Kumar; Chakraborty, Pampi; Kumar, Santosh; Singh, Prafull Kumar; Rajan, M G R; Sainis, Krishna B; Kulkarni, Savita

    2016-01-01

    Rapid emergence of drug resistance in Mycobacterium tuberculosis (MTB) is a major health concern and demands the development of novel adjunct immunotherapeutic agents capable of modulating the host immune responses in order to control the pathogen. In the present study, we sought to investigate the immunomodulatory effects of G1-4A, a polysaccharide derived from the Indian medicinal plant Tinospora cordifolia, in in-vitro and aerosol mouse models of MTB infection. G1-4A treatment of MTB infected RAW264.7 macrophages significantly induced the surface expression of MHC-II and CD-86 molecules, secretion of proinflammatory cytokines (TNF-α, IL-β, IL-6, IL-12, IFN-γ) and nitric oxide leading to reduced intracellular survival of both drug sensitive (H37Rv) as well as multi drug resistant strains (Beijing and LAM) of MTB, which was partially attributed to G1-4A induced NO production in TLR4-MyD88 dependent manner. Similarly, bacillary burden was significantly reduced in the lungs of MTB infected BALB/c mice treated with G1-4A, with simultaneous up-regulation of the expression of TNF-α, INF-γ and NOS2 in the mouse lung along with increased levels of Th1 cytokines like IFN-γ, IL-12 and decreased levels of Th2 cytokine like IL-4 in the serum. Furthermore, combination of G1-4A with Isoniazid (INH) exhibited better protection against MTB compared to that due to INH or G1-4A alone, suggesting its potential as adjunct therapy. Our results demonstrate that modulation of host immune responses by G1-4A might improve the therapeutic efficacy of existing anti-tubercular drugs and provide an attractive strategy for the development of alternative therapies to control tuberculosis. PMID:27148868

  3. INTRODUCTION Summary of Papers Summary of Papers

    Science.gov (United States)

    Gauthier, Serge; Abarzhi, Snezhana I.; Sreenivasan, Katepalli R.

    2010-12-01

    review various aspects of Turbulent Mixing that were discussed at the Second International Conference and Advanced School 'Turbulent Mixing and Beyond', TMB-2009, held in summer 2009 at the Abdus Salam International Centre for Theoretical Physics (ICTP), Trieste, Italy. The papers are arranged by TMB themes and within each theme they are ordered alphabetically by the last name of the first author, with tutorials following research contributions. Canonical turbulence and turbulent mixing. The theme of canonical turbulence and turbulent mixing is considered by several authors. Casciola et al investigate the dynamics of inertial particles dispersed in a turbulent jet and compare their numerical modeling results with the classical similarity theory of the jet far-field. Remarkable agreement is found between the theory and the direct numerical simulations (DNS), including decay of Stokes numbers with the distance from the origin, self-similarity of the mean axial particle velocity profile, etc. Nagata considers complex turbulent flows, which are known to exhibit no linear critical point for the laminar states, and which are linearly stable at finite Reynolds numbers. Square duct flow and sliding Couette flow in an annulus are considered and nonlinear traveling-wave states are found for the flows with the use of the homotopy approach developed by the author. These states may constitute a skeleton around which a time-dependent trajectory in the phase space is organized. Teitelbaum and Mininni study a decaying 3D incompressible turbulence, which mimicks turbulent mixing in geophysical flows, with rotation rendering the flow anisotropic at large scales. The authors analyze three DNS results (without and with rotation, and with helicity), observe a decoupling of the modes normal to the rotation axis, and show that the helicity decreases the decay rate of turbulence. Wang and Peters investigate the structure of turbulence by studying strain rates of various scalars, including a

  4. Simultaneous determination of aflatoxin B(1), B(2), G(1), G(2), ochratoxin A, and sterigmatocystin in traditional Chinese medicines by LC-MS-MS.

    Science.gov (United States)

    Zheng, Runsheng; Xu, Hui; Wang, Wenli; Zhan, Ruoting; Chen, Weiwen

    2014-05-01

    In this paper we describe a rapid, simple, and costeffective liquid chromatography–tandem mass spectrometric (LC–MS–MS) method for simultaneous analysis of aflatoxin B1, B2, G1, and G2, ochratoxin A, and sterigmatocystin in 25 traditional Chinese medicines (TCMs). The method is based on single extraction with 84:16 (v/v) acetonitrile–water then analysis of the diluted crude extract without further clean-up. Chromatographic separation was achieved on a C18 column, with a mobile phase gradient prepared from aqueous 4 mmol L−1 ammonium acetate–0.1 % formic acid and methanol. Quantification of the analytes was by selective reaction monitoring (SRM) on a triple-quadrupole mass spectrometer in positive-ionization mode. Special focus was on investigating and reducing matrix effects to improve accuracy. The established method was validated by determination of linearity (r>0.995), sensitivity (limits of quantification 1.6–25.0 ng L−1), apparent recovery (84.8–110.6 %), extraction recovery (83.6–106.1 %), and precision (relative standard deviation ≤9.9 %) for two representative TCMs, Semen Armeniacae Amarae and Radix Pseudostellariae. The applicability of the method to TCMs other than these was further investigated, and 23 other TCMs with acceptable matrix effects (80.2–118.6 %) were screened. The validated method was finally used to assess mycotoxin contamination of 244 samples of 25 TCMs collected from local hospitals and TCM pharmacies. Aflatoxin B1 and ochratoxin A were detected in 5.3 % of the samples. Sterigmatocystin, the most prevalent mycotoxin contaminant, was present in 26.2 % of the samples tested; this has not been reported previously. The results of this work imply greater attention should be devoted to evaluation of the potential hazard caused by sterigmatocystin in TCMs.

  5. Simultaneous determination of aflatoxin B(1), B(2), G(1), G(2), ochratoxin A, and sterigmatocystin in traditional Chinese medicines by LC-MS-MS.

    Science.gov (United States)

    Zheng, Runsheng; Xu, Hui; Wang, Wenli; Zhan, Ruoting; Chen, Weiwen

    2014-05-01

    In this paper we describe a rapid, simple, and costeffective liquid chromatography–tandem mass spectrometric (LC–MS–MS) method for simultaneous analysis of aflatoxin B1, B2, G1, and G2, ochratoxin A, and sterigmatocystin in 25 traditional Chinese medicines (TCMs). The method is based on single extraction with 84:16 (v/v) acetonitrile–water then analysis of the diluted crude extract without further clean-up. Chromatographic separation was achieved on a C18 column, with a mobile phase gradient prepared from aqueous 4 mmol L−1 ammonium acetate–0.1 % formic acid and methanol. Quantification of the analytes was by selective reaction monitoring (SRM) on a triple-quadrupole mass spectrometer in positive-ionization mode. Special focus was on investigating and reducing matrix effects to improve accuracy. The established method was validated by determination of linearity (r>0.995), sensitivity (limits of quantification 1.6–25.0 ng L−1), apparent recovery (84.8–110.6 %), extraction recovery (83.6–106.1 %), and precision (relative standard deviation ≤9.9 %) for two representative TCMs, Semen Armeniacae Amarae and Radix Pseudostellariae. The applicability of the method to TCMs other than these was further investigated, and 23 other TCMs with acceptable matrix effects (80.2–118.6 %) were screened. The validated method was finally used to assess mycotoxin contamination of 244 samples of 25 TCMs collected from local hospitals and TCM pharmacies. Aflatoxin B1 and ochratoxin A were detected in 5.3 % of the samples. Sterigmatocystin, the most prevalent mycotoxin contaminant, was present in 26.2 % of the samples tested; this has not been reported previously. The results of this work imply greater attention should be devoted to evaluation of the potential hazard caused by sterigmatocystin in TCMs. PMID:24658469

  6. 49 CFR Appendix to Part 38 - Guidance Material

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Guidance Material Appendix to Part 38... SPECIFICATIONS FOR TRANSPORTATION VEHICLES Pt. 38, App. Appendix to Part 38—Guidance Material This appendix....5. A research project sponsored by the Architectural and Transportation Barriers Compliance...

  7. 40 CFR Appendix B to Part 66 - Instruction Manual

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 15 2010-07-01 2010-07-01 false Instruction Manual B Appendix B to Part 66 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED... Manual Note: For text of appendix B see appendix B to part 67....

  8. CATV Technical Papers.

    Science.gov (United States)

    National Cable Television Association, Inc., Washington, DC.

    Complete technical papers of the 1977 National Cable Television Association Convention are included in this report. Twenty-two of 32 papers and two abstracts cover the topics of advanced cable television techniques, human reactions to television picture impairment, special displays and services, protection from theft of service, everyday…

  9. Transportation: Topic Paper E.

    Science.gov (United States)

    National Council on the Handicapped, Washington, DC.

    As one of a series of topic papers assessing federal laws and programs affecting persons with disabilities, this paper reviews the issue of transportation services. In the area of urban mass transit, four relevant pieces of legislation and public transportation accessibility regulations are cited, and cost issues are explored. Paratransit systems,…

  10. DISSERTATION AS QUALIFICATION PAPER

    Directory of Open Access Journals (Sweden)

    A. P. Nesenchuk

    2008-01-01

    Full Text Available The paper considers problems pertaining to methodology and structure for dissertation preparation. All the problems have been solved on the basis of the analysis of questionnaire complexes. The paper presents concrete examples taken from the dissertations that have passed expertise of the Highest Certifying Commission.

  11. Printed paper photovoltaic cells

    Energy Technology Data Exchange (ETDEWEB)

    Huebler, Arved; Trnovec, Bystrik; Zillger, Tino; Ali, Moazzam; Wetzold, Nora [Inistitute for Print and Media Technology, Chemnitz University of Technology, Chemnitz (Germany); Mingebach, Markus; Wagenpfahl, Alexander; Deibel, Carsten [Experimental Physics VI, Julius-Maximilians-University of Wuerzburg (Germany); Dyakonov, Vladimir [Experimental Physics VI, Julius-Maximilians-University of Wuerzburg (Germany); Bavarian Center for Applied Energy Research e.V. (ZAE Bayern), Wuerzburg (Germany)

    2011-11-15

    Polymer/fullerene solar cells are printed on paper using a combination of gravure and flexographic printing techniques. The printed paper photovoltaic cells are free from expensive electrodes made with indium-tin oxide, silver, or gold. Oxidized zinc film is used as the electron-collecting layer. (Copyright copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  12. Paper Highlight — Site

    Science.gov (United States)

    In a recent paper published in Cancer Cell, ICBP investigators at the University of Texas Health Science Center, want to provide the potential mechanistic explanation how the known 20q13 and 17q23 amplicons transcriptionally control gene expression through long-range chromatin proximity. This paper describes how different large sets of data can be integrated for finding new biological mechanisms.

  13. Wrinkling of wet paper

    Science.gov (United States)

    Kim, Ho-Young; Kim, Jungchul; Mahadevan, L.

    2011-11-01

    It is a mundane experience that paper stained with water wrinkles. It is because a wetted portion of paper, which swells due to the hygroexpansive nature of the cellulose fiber network, deforms out of its original plane. Here we quantify the dynamics of wrinkling of wet paper coupled to the capillary imbibition of water into paper using a combination of experiment and theory. While supplying water from a capillary tube that touches the center of a paper strip, we measure the spreading rate of the wet area, wait time for the out-of-plane buckling, and temporal growth of a wrinkling magnitude. Using a theoretical model assuming a linear increase of the strain and an exponential decay of the elastic modulus with the water concentration, we construct scaling laws to predict the simultaneous capillary imbibition and wrinkling rates. This work was supported by the Wyss Institute of Harvard University.

  14. Overexpression of a glycosyltransferase gene SrUGT74G1 from Stevia improved growth and yield of transgenic Arabidopsis by catechin accumulation.

    Science.gov (United States)

    Guleria, Praveen; Yadav, Sudesh Kumar

    2014-03-01

    Steviol glycoside and gibberellin biosynthetic routes are known as divergent branches of a common origin in Stevia. A UDP-glycosyltransferase encoded by SrUGT74G1 catalyses the conversion of steviolbioside into stevioside in Stevia rebaudiana leaves. In the present study, transgenic Arabidopsis thaliana overexpressing SrUGT74G1 cDNA from Stevia were developed to check the probability of stevioside biosynthesis in them. However, stevioside accumulation was not evident in transgenics. Also, the transgenic Arabidopsis showed no change in GA3 content on SrUGT74G1 overexpression. Surprisingly, significant accumulation of catechin was noticed in transgenics. The transgenics showed a considerable increase in shoot length, root length and rosette area. An increase in free radical scavenging activity of transgenics was noticed. Moreover, the seed yield of transgenics was also increased by 6-15% than control. Additionally, variation in trichome branching pattern on leaf surface of transgenics was observed. The trichome branching pattern was also validated by exogenous catechin exposure (10, 50, 100 ng ml(-1)) to control plants. Hence, present study reports the probable role of SrUGT74G1 from Stevia in catechin accumulation of transgenic Arabidopsis thaliana. Thus, detailed study in present perspective has revealed the role of Stevia SrUGT74G1 gene in trichome branching pattern, improved vegetative growth, scavenging potential and seed yield by catechin accumulation in transgenic Arabidopsis.

  15. Murine germinal center B cells require functional Fms-like tyrosine kinase 3 signaling for IgG1 class-switch recombination.

    Science.gov (United States)

    Svensson, Mattias N D; Andersson, Karin M E; Wasén, Caroline; Erlandsson, Malin C; Nurkkala-Karlsson, Merja; Jonsson, Ing-Marie; Brisslert, Mikael; Bemark, Mats; Bokarewa, Maria I

    2015-12-01

    Switched antibody classes are important for efficient immune responses. Aberrant antibody production to otherwise harmless antigens may result in autoimmunity. The protein kinase fms-like tyrosine kinase 3 receptor (Flt3) has an important role during early B-cell development, but the role of Flt3 in peripheral B cells has not been assessed before. Herein we describe a previously unappreciated role for Flt3 in IgG1 class-switch recombination (CSR) and production. We show that Flt3 is reexpressed on B-cell lymphoma 6(+) germinal center B cells in vivo and following LPS activation of peripheral B cells in vitro. Absence of Flt3 signaling in Flt3 ligand-deficient mice results in impaired IgG1 CSR and accumulation of IgM-secreting plasma cells. On activated B cells, Flt3 is coexpressed and functions in synergy with the common-gamma chain receptor family. B cells from Flt3 ligand-deficient mice have impaired IL-4R signaling, with reduced phosphorylation of signal transducer and activator of transcription (Stat) 6, and demonstrate a failure to initiate CSR to IgG1 with low expression of γ1 germ-line transcripts, resulting in impaired IgG1 production. Thus, functional synergy between Flt3 and IL-4R signaling is critical for Stat-mediated regulation of sterile γ1 germ-line transcripts and CSR to IgG1. PMID:26627255

  16. 12 CFR Appendixes A-H to Subpart A... - Appendixes A-H to Subpart A of Part 702

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Appendixes A-H to Subpart A of Part 702 A Appendixes A-H to Subpart A of Part 702 Banks and Banking NATIONAL CREDIT UNION ADMINISTRATION REGULATIONS AFFECTING CREDIT UNIONS PROMPT CORRECTIVE ACTION Net Worth Classification Pt. 702, Apps. Appendixes A-H...

  17. Appendix

    DEFF Research Database (Denmark)

    Friis, Ib; Thulin, Mats

    2009-01-01

    A taxonomic and floristic account of all the additional or new species in Etyhiopia and/or Eritrea that have been discovered while Vols 2 - 7 of the Flora of Ethiopia and Eritrea were being published....

  18. Sri Lanka; Background Papers

    OpenAIRE

    International Monetary Fund

    1995-01-01

    This Background Paper on Sri Lanka provides information on the economic developments during 1992–95. Developments in the domestic and external sectors are discussed. The deficiencies of the official consumer price index that resulted in a substantial understatement of inflation performance in 1994 and alternative estimates of underlying inflation are described. The structural rigidities in the labor market that perpetuate high unemployment and limit job growth are also described. The paper ...

  19. Peru; Selected Issues Paper

    OpenAIRE

    International Monetary Fund

    2012-01-01

    This Selected Issues paper examines application of fiscal framework alternatives to the case of Peru. Although growth has been at historical highs over the last decade, Peru still has an important infrastructure gap and a quarter of its population still lives in poverty. This paper applies illustratively new modified frameworks recently developed by the IMF to the case of Peru. It takes stock of analytical considerations to resource management, and analyzes Peru’s natural resource wealth an...

  20. Nanotechnology in paper electronics

    Science.gov (United States)

    Demming, Anna; Österbacka, Professor Ronald; Han, Jin-Woo, Dr

    2014-03-01

    The ability to put cutting edge technology on paper—not in words but in a working physical form—has been attracting an increasing number of researchers over the past decade. Paper has many advantages that make it attractive for flexible electronics: it is relatively environmentally benign; it is renewable; it can be recycled; it is light weight; production processes for paper are well advanced; and it is inexpensive. This special issue, with guest editors Ronald Österbacka from Åbo Akademi University in Finland and Jin-Woo Han from the NASA AMES Research Center, features some of the latest in paper electronics research, including developments towards applications in displays, sensing and alternative energy sources, as well as fundamental studies to further our understanding of how paper can be most effectively used in electronics. As Andrew Steckl and colleagues in the US point out, 'Cellulose-based paper substrates were implemented as an electronic substrate as early as 1969, with most advancement occurring in the past decade largely due to technology improvements in thin film deposition and organic materials' [1, 2]. They report a detailed comparison between paper, standard liquid crystal display rigid glass and flexible glass for hosting pentacene organic thin film transistors, and obtain promising results for future paper-based devices. As most meaningful electronic devices rely on transistors to function, transistors feature quite prominently in this special issue. Rodrigo Martins and colleagues in France and Portugal study the effect of fibre type, structure and dimension on paper-based transistors and reveal further insights into how paper properties affect device performance [3]. Qing Wan and colleagues in China bring the state of the art in transistor technology to paper substrates [4], fabricating indium-zinc-oxide (IZO)-based protonic/electronic hybrid thin film transistors on paper and showing that they can be used as artificial synapses. Like the

  1. Paper Gains and Losses

    Institute of Scientific and Technical Information of China (English)

    ADAMMINTER

    2004-01-01

    Wet piles of old cardboard are stacked on both sides of a loading platform, leaving just enough room for a truck to stop between them. Zhang Guanan, the stocky general manager of the Liudian General Paper Mill Factory, stands in the middle of the space. Behind him is the roar of a paper mill and a long flatbed truck leaving a warehouse loaded with twenty-eight giant rolls of carton board destined for a box

  2. Quantum interaction. Selected papers

    Energy Technology Data Exchange (ETDEWEB)

    Atmanspacher, Harald [Eidgenoessische Technische Hochschule, Zurich (Switzerland); Haven, Emmanuel [Leicester Univ. (United Kingdom). School of Management; Kitto, Kirsty [Queensland Univ. of Technology, Brisbane, QLD (Australia); Raine, Derek (ed.) [Leicester Univ. (United Kingdom). Centre for Interdisciplinary Science

    2014-07-01

    This book constitutes the refereed proceedings of the 7th International Conference on Quantum Interaction, QI 2013, held in Leicester, UK, in July 2013. The 31 papers presented in this book were carefully selected from numerous submissions. The papers cover various topics on quantum interaction and revolve around four themes: information processing/retrieval/semantic representation and logic; cognition and decision making; finance/economics and social structures and biological systems.

  3. Abstracts of contributed papers

    Energy Technology Data Exchange (ETDEWEB)

    1994-08-01

    This volume contains 571 abstracts of contributed papers to be presented during the Twelfth US National Congress of Applied Mechanics. Abstracts are arranged in the order in which they fall in the program -- the main sessions are listed chronologically in the Table of Contents. The Author Index is in alphabetical order and lists each paper number (matching the schedule in the Final Program) with its corresponding page number in the book.

  4. Mexico; Selected Issues Paper

    OpenAIRE

    International Monetary Fund

    2010-01-01

    This Selected Issues paper analyzes the global crisis and potential growth in Mexico. The paper uses two methodologies to assess to what extent the global crisis is likely to weigh on Mexico’s growth potential. The first approach is sectoral, examining the historical relationship between financial stress and growth in manufacturing industries. The second approach uses a growth-accounting framework to take a closer look at likely developments in the factors that drive potential growth. The p...

  5. Online Paper Review Analysis

    Directory of Open Access Journals (Sweden)

    Doaa Mohey El-Din

    2015-09-01

    Full Text Available Sentiment analysis or opinion mining is used to automate the detection of subjective information such as opinions, attitudes, emotions, and feelings. Hundreds of thousands care about scientific research and take a long time to select suitable papers for their research. Online reviews on papers are the essential source to help them. The reviews save reading time and save papers cost. This paper proposes a new technique to analyze online reviews. It is called sentiment analysis of online papers (SAOOP. SAOOP is a new technique used for enhancing bag-of-words model, improving the accuracy and performance. SAOOP is useful in increasing the understanding rate of review's sentences through higher language coverage cases. SAOOP introduces solutions for some sentiment analysis challenges and uses them to achieve higher accuracy. This paper also presents a measure of topic domain attributes, which provides a ranking of total judging on each text review for assessing and comparing results across different sentiment techniques for a given text review. Finally, showing the efficiency of the proposed approach by comparing the proposed technique with two sentiment analysis techniques. The comparison terms are based on measuring accuracy, performance and understanding rate of sentences.

  6. Post-transcriptional regulation of cyclins D1, D3 and G1 and proliferation of human cancer cells depend on IMP-3 nuclear localization.

    Science.gov (United States)

    Rivera Vargas, T; Boudoukha, S; Simon, A; Souidi, M; Cuvellier, S; Pinna, G; Polesskaya, A

    2014-05-29

    RNA-binding proteins of the IMP family (insulin-like growth factor 2 (IGF2) mRNA-binding proteins 1-3) are important post-transcriptional regulators of gene expression. Multiple studies have linked high expression of IMP proteins, and especially of IMP-3, to an unfavorable prognosis in numerous types of cancer. The specific importance of IMP-3 for cancer transformation remains poorly understood. We here show that all three IMPs can directly bind the mRNAs of cyclins D1, D3 and G1 (CCND1, D3 and G1) in vivo and in vitro, and yet only IMP-3 regulates the expression of these cyclins in a significant manner in six human cancer cell lines of different origins. In the absence of IMP-3, the levels of CCND1, D3 and G1 proteins fall dramatically, and the cells accumulate in the G1 phase of the cell cycle, leading to almost complete proliferation arrest. Our results show that, compared with IMP-1 and IMP-2, IMP-3 is enriched in the nucleus, where it binds the transcripts of CCND1, D3 and G1. The nuclear localization of IMP-3 depends on its protein partner HNRNPM and is indispensable for the post-transcriptional regulation of expression of the cyclins. Cytoplasmic retention of IMP-3 and HNRNPM in human cancer cells leads to significant drop in proliferation. In conclusion, a nuclear IMP-3-HNRNPM complex is important for the efficient synthesis of CCND1, D3 and G1 and for the proliferation of human cancer cells.

  7. PP2A-mediated regulation of Ras signaling in G2 is essential for stable quiescence and normal G1 length

    OpenAIRE

    Naetar, Nana; Soundarapandian, Velmurugan; Litovchick, Larisa; Goguen, Kelsey L.; Sablina, Anna A; Bowman-Colin, Christian; Sicinski, Piotr; Hahn, William C.; DeCaprio, James A.; Livingston, David M.

    2014-01-01

    Quiescence (G0) allows cycling cells to reversibly cease proliferation. A decision to enter quiescence is suspected of occurring early in G1, before the restriction point, R. Surprisingly, we have identified G2 as an interval during which inhibition of the protein phosphatase, PP2A, results in failure to exhibit stable quiescence. This effect is accompanied by shortening of the ensuing G1. The PP2A subcomplex required for stable G0 contains the B56γ B subunit. Following PP2A inhibition in G2,...

  8. Draft genome sequences of two Kocuria isolates, K. salsicia G1 and K. rhizophila G2, isolated from a slaughterhouse in Denmark

    DEFF Research Database (Denmark)

    Herschend, Jakob; Raghupathi, Prem Krishnan; Røder, Henriette Lyng;

    2016-01-01

    We report here the draft genome sequences ofKocuria salsiciaG1 andKocuria rhizophilaG2, which were isolated from a meat chopper at a small slaughterhouse in Denmark. The two annotated genomes are 2.99 Mb and 2.88 Mb in size, respectively.......We report here the draft genome sequences ofKocuria salsiciaG1 andKocuria rhizophilaG2, which were isolated from a meat chopper at a small slaughterhouse in Denmark. The two annotated genomes are 2.99 Mb and 2.88 Mb in size, respectively....

  9. Call for Papers

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    <正>Internet Law Review is a scholarly monographic series focused on the research of internet law.It is sponsored by School of Intellectual Property,Peking University,and Internet Law Center of Peking University,edited by Professor Zhang Ping,and published twice a year. We are now preparing for the forthcoming issue of the Review,Volume 13,and prospective authors with new ideas,comprehensive and sound research methods and theories,and great creativity,are invited to submit your articles.Also,we strongly welcome excellent papers with high practicality and supported by detailed examples,and critical review papers to

  10. Extending Paper Chromatography Inquiry

    Science.gov (United States)

    Finson, Kevin

    2004-01-01

    One of the "good old" standard activities middle school students seem to enjoy is paper chromatography. The procedures and materials needed are relatively simple and the results can be colorful. All too often, the activity ends just after these colorful results are obtained, cutting short the potential it holds for some further inquiry. With some…

  11. Working Paper No.1

    Science.gov (United States)

    Graff, Harvey J.; Street, Brian V.; Jones, Stan

    1998-01-01

    This document is a collection of three parts: two extended papers and one shorter commentary on the International Adult Literacy Survey (IALS). These originally appeared in "Literacy Across the Curriculum" in response to an invitation for comment to several prominent international researchers. The first of these entitled "The…

  12. From Paper to PDA

    DEFF Research Database (Denmark)

    Kanstrup, Anne Marie; Stage, Jan

    2009-01-01

    Mobile devices with small screens and minimal facilities for interaction are increasingly being used in complex human activities for accessing and processing information, while the user is moving. This paper presents a case study of the design and evaluation of a mobile system, which involved...

  13. Pencil and paper

    DEFF Research Database (Denmark)

    Wong, Bang; Kjærgaard, Rikke Schmidt

    2012-01-01

    Creating pictures is integral to scientific thinking. In the visualization process, putting pencil to paper is an essential act of inward reflec- tion and outward expression. It is a constructive activity that makes our thinking specific and explicit. Compared to other constructive approaches...

  14. A Position Paper.

    Science.gov (United States)

    Hixson, Richard A.

    This position paper covers possible problems of collective bargaining. (1) The two sides should not bring prejudgments of good or bad to the negotiating table. (2) Neither side should exaggerate its strength or minimize its weakness. (3) Neither side should confuse intransigence with firmness. (4) The composition of each team must be carefully…

  15. Library Services. Miscellaneous Papers.

    Science.gov (United States)

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library journal cooperation, interlibrary lending, library services to minorities, and school library media centers, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Co-operation between Editors of Library Journals in Socialist Countries," in which Wolfgang Korluss…

  16. Abstracts of Selected Papers

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    The present inflation is compound inflation influenced by internal and external factors. The paper brings forward three stages of supply changes of working-age population: 1) Not until 2010, the first stage can be described as a buyer's market of labor, i

  17. Regional Activities Division. Papers.

    Science.gov (United States)

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library network activities in Canada, the Third World, Japan, Malaysia, Brazil, and Sweden which were presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Canada: A Voluntary and Flexible Network," a review by Guy Sylvestre of the political, social, and economic structures affecting…

  18. Abstracts of submitted papers

    International Nuclear Information System (INIS)

    The conference proceedings contain 152 abstracts of presented papers relating to various aspects of personnel dosimetry, the dosimetry of the working and living environment, various types of dosemeters and spectrometers, the use of radionuclides in various industrial fields, the migration of radionuclides on Czechoslovak territory after the Chernobyl accident, theoretical studies of some parameters of ionizing radiation detectors, and their calibration. (M.D.)

  19. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National

  20. Two three pages papers

    NARCIS (Netherlands)

    K.R. Apt

    2012-01-01

    Dexter was my manager during the first 8 months or so of my one year stay at the IBM Research Center at Yorktown Heights in the mid eighties. Our interaction in terms of scientific output was very slim — it consisted of just a single paper, titled "Limits for automatic verification of finite-state c

  1. Intrinsic Properties of immunoglobulin IgG1 Isotype-Switched B Cell Receptors Promote Microclustering and the Initiation of Signaling

    Science.gov (United States)

    Liu, Wanli; Meckel, Tobias; Tolar, Pavel; Sohn, Hae Won; Pierce, Susan K.

    2010-01-01

    Summary Memory B cells express high affinity, immunoglobulin B cell receptors (IgG-BCRs) that enhance B cell responses giving rise to the rapid production of high affinity, IgG antibodies. Despite the central role of IgG-BCRs in memory responses, the mechanisms by which the IgG-BCRs function to enhance B cell responses are not fully understood. Using high-resolution live-cell imaging we showed that independent of affinity, IgG1-BCRs dramatically enhanced the earliest BCR-intrinsic events that followed within seconds of B cells’ encounter with membrane bound antigen including BCR oligomerization and BCR microcluster growth, leading to Syk kinase recruitment and calcium responses. The enhancement of these early events was dependent on a membrane proximal region of the IgG1 cytoplasmic tail not previously appreciated to play a role in IgG1-BCR signaling. Thus, intrinsic properties of the IgG1-BCR enhance early antigen-driven events that ultimately translate into heightened signaling. PMID:20620943

  2. Comparison of Kil Goat and Saanen x Kil Goat Crosbred (F1, G1 Raised At The Farm Conditions In Terms of Fertility Characteristics

    Directory of Open Access Journals (Sweden)

    Hilal Tozlu Çelik

    2015-01-01

    Full Text Available This research Amasya Sarilar (40°54'23"N, 35°08'min5"E, a private business in the years 2011-2012 between the grown Saanen x Kil goat crosbred (F1, G1 and Kil goat in the fertility characteristics to detect and genotype on these features, year was conducted to determine the effects of such factors. In the study of reproductive traits for the year 2011 only twin goats giving birth rate among genotypes were found statistically difference. The twin birth rate of Kil goat was similar in F1 crosbred and G1 is different from both genotypes were found. In 2012, the remaining infertile among genotypes goat, dead goat giving birth and twin goats rate has been determined that there are statistical differences. Infertility rate, Saanen x Kil goat F1, G1 crosbred was found to be higher than in the Kil goat. Twin goat giving birth rate F1 genotypes in high, genotypes G1 and Kil goats find similar.

  3. Characterization of a G1P[8] rotavirus causing an outbreak of gastroenteritis in the Northern Territory, Australia, in the vaccine era

    Science.gov (United States)

    Donato, Celeste M; Cowley, Daniel; Snelling, Thomas L; Akopov, Asmik; Kirkness, Ewen F; Kirkwood, Carl D

    2014-01-01

    In 2010, a large outbreak of rotavirus gastroenteritis occurred in the Alice Springs region of the Northern Territory, Australia. The outbreak occurred 43 months after the introduction of the G1P[8] rotavirus vaccine Rotarix®. Forty-three infants were hospitalized during the outbreak and analysis of fecal samples from each infant revealed a G1P[8] rotavirus strain. The outbreak strain was adapted to cell culture and neutralization assays were performed using VP7 and VP4 neutralizing monoclonal antibodies. The outbreak strain exhibited a distinct neutralization resistance pattern compared to the Rotarix® vaccine strain. Whole genome sequencing of the 2010 outbreak virus strain demonstrated numerous amino acid differences compared to the Rotarix® vaccine strain in the characterized neutralization epitopes of the VP7 and VP4 proteins. Phylogenetic analysis of the outbreak strain revealed a close genetic relationship to global strains, in particular RVA/Human-wt/BEL/BE0098/2009/G1P[8] and RVA/Human-wt/BEL/BE00038/2008/G1P[8] for numerous genes. The 2010 outbreak strain was likely introduced from a globally circulating population of strains rather than evolving from an endemic Australian strain. The outbreak strain possessed antigenic differences in the VP7 and VP4 proteins compared to the Rotarix® vaccine strain. The outbreak was associated with moderate vaccine coverage and possibly low vaccine take in the population. PMID:26038746

  4. Characterization of a G1P[8] rotavirus causing an outbreak of gastroenteritis in the Northern Territory, Australia, in the vaccine era.

    Science.gov (United States)

    Donato, Celeste M; Cowley, Daniel; Snelling, Thomas L; Akopov, Asmik; Kirkness, Ewen F; Kirkwood, Carl D

    2014-07-01

    In 2010, a large outbreak of rotavirus gastroenteritis occurred in the Alice Springs region of the Northern Territory, Australia. The outbreak occurred 43 months after the introduction of the G1P[8] rotavirus vaccine Rotarix(®). Forty-three infants were hospitalized during the outbreak and analysis of fecal samples from each infant revealed a G1P[8] rotavirus strain. The outbreak strain was adapted to cell culture and neutralization assays were performed using VP7 and VP4 neutralizing monoclonal antibodies. The outbreak strain exhibited a distinct neutralization resistance pattern compared to the Rotarix(®) vaccine strain. Whole genome sequencing of the 2010 outbreak virus strain demonstrated numerous amino acid differences compared to the Rotarix(®) vaccine strain in the characterized neutralization epitopes of the VP7 and VP4 proteins. Phylogenetic analysis of the outbreak strain revealed a close genetic relationship to global strains, in particular RVA/Human-wt/BEL/BE0098/2009/G1P[8] and RVA/Human-wt/BEL/BE00038/2008/G1P[8] for numerous genes. The 2010 outbreak strain was likely introduced from a globally circulating population of strains rather than evolving from an endemic Australian strain. The outbreak strain possessed antigenic differences in the VP7 and VP4 proteins compared to the Rotarix(®) vaccine strain. The outbreak was associated with moderate vaccine coverage and possibly low vaccine take in the population. PMID:26038746

  5. Susceptibility and intra-species transmission of the H9N2 G1 prototype lineage virus in Japanese quail and turkeys.

    Science.gov (United States)

    Bonfante, Francesco; Patrono, Livia Victoria; Aiello, Roberta; Beato, Maria Serena; Terregino, Calogero; Capua, Ilaria

    2013-07-26

    Avian influenza viruses of the H9N2 subtype have circulated in the poultry population in Asia, Far and Middle East since the mid-1990 s. One of the most widespread lineages established in poultry is the G1 lineage. This lineage has undergone further evolution and reassortment since its first detection in 1997 and G1-like H9N2 viruses still circulate. In this study we have investigated the susceptibility of quail and turkeys to the H9N2 G1-lineage prototype strain (A/quail/Hong Kong/G1/97). Contact transmission experiments were carried out in both avian species. Animals were infected oro-nasally with increasing doses of the virus (10(3)-10(6) EID 50/0.1 ml) and sentinel birds were introduced 4 days post infection (pi) in each experimental group. Quail were more susceptible than turkeys, as they were readily infected with lower challenge doses. Interestingly, infection of turkeys was associated with worse clinical condition. Transmission was detected in both species. Quail infected with a dose less than or equal to 10(4) EID50 transmitted the virus to the sentinels without showing any signs of disease. These findings reinforce the hypothesis that quail may ensure the perpetuation of H9N2 viruses in poultry, acting as a silent reservoir. PMID:23597652

  6. G protein-coupled receptor 30 ligand G-1 increases aryl hydrocarbon receptor signalling by inhibition of tubulin assembly and cell cycle arrest in human MCF-7 cells.

    Science.gov (United States)

    Tarnow, Patrick; Tralau, Tewes; Luch, Andreas

    2016-08-01

    Regulatory crosstalk between the aryl hydrocarbon receptor (AHR) and oestrogen receptor α (ERα) is well established. Apart from the nuclear receptors ERα and ERβ, oestrogen signalling further involves an unrelated G protein-coupled receptor termed GPR30. In order to investigate potential regulatory crosstalk, this study investigated the influence of G-1 as one of the few GPR30-specific ligands on the AHR regulon in MCF-7 cells. As a well-characterised model system, these human mammary carcinoma cells co-express all three receptors (AHR, ERα and GPR30) and are thus ideally suited to study corresponding regulatory pathway interactions on transcript level. Indeed, treatment with micromolar concentrations of the GPR30-specific agonist G-1 resulted in up-regulation of AHR as well as the transcripts for cytochromes P450 1A1 and 1B1, two well-known targets of the AHR regulon. While this was partly attributable to G-1-mediated inhibition of tubulin assembly and subsequent cell cycle arrest in the G2/M phase, the effects nevertheless required functional AHR. However, G-1-induced up-regulation of CYP 1A1 was not mediated by GPR30, as G15 antagonist treatment as well as a knockdown of GPR30 and AHR failed to inhibit this effect. PMID:26475489

  7. Regulation of the G1/S Transition in Hepatocytes: Involvement of the Cyclin-Dependent Kinase Cdk1 in the DNA Replication

    Directory of Open Access Journals (Sweden)

    Anne Corlu

    2012-01-01

    Full Text Available A singular feature of adult differentiated hepatocytes is their capacity to proliferate allowing liver regeneration. This review emphasizes the literature published over the last 20 years that established the most important pathways regulating the hepatocyte cell cycle. Our article also aimed at illustrating that many discoveries in this field benefited from the combined use of in vivo models of liver regeneration and in vitro models of primary cultures of human and rodent hepatocytes. Using these models, our laboratory has contributed to decipher the different steps of the progression into the G1 phase and the commitment to S phase of proliferating hepatocytes. We identified the mitogen dependent restriction point located at the two-thirds of the G1 phase and the concomitant expression and activation of both Cdk1 and Cdk2 at the G1/S transition. Furthermore, we demonstrated that these two Cdks contribute to the DNA replication. Finally, we provided strong evidences that Cdk1 expression and activation is correlated to extracellular matrix degradation upon stimulation by the pro-inflammatory cytokine TNFα leading to the identification of a new signaling pathway regulating Cdk1 expression at the G1/S transition. It also further confirms the well-orchestrated regulation of liver regeneration via multiple extracellular signals and pathways.

  8. Repairability during G 1 phase of inducting lesions of sister chromatid exchange produced by mitomycin C in salivary gland cells of mice In Vivo

    International Nuclear Information System (INIS)

    The repairability of the injuries that lead to the formation of sister chromatid exchange (SCE) produced by mitomycin C (MMC) with a dose of 2.1 mg/g in vivo, during the G 1 phase in the first cycle of cellular division (before the incorporation of BrdU [5-bromo-2 deoxyurine] to the DNA), as well as during the G 1 phase of the second cycle of cellular division (after the incorporation of BrdU) were analyzed. A 35.1% decrease in the frequency of SCE produced by Mitomycin C was observed, in the early G 1 phase of the first division, with respect to the frequency of SCE induced in the later G 1 phase. When Mitomycin C is given to cells whose DNA is substituted with BrdU in only one of the chain's filaments such decrease is not observed. The results suggest that the injuries caused by MMC, which give place to the SCE, in cells of the salivary glands of the mouse in vivo, are partially repaired only when induced in DNA which has not been substituted with BrdU. (Author)

  9. Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.

    Science.gov (United States)

    Assad, Aryan; Amann, Barbara; Friedrich, Annette; Deeg, Cornelia Annette

    2012-06-15

    Bovine neonatal pancytopenia (BNP) is mainly characterized by multiple haemorrhages, thrombocytopenia and leukocytopenia as a result of bone marrow depletion. BNP can be induced in healthy calves through application of colostrum from BNP donors, proofing that BNP is mediated to maternal alloantibodies. Alloantibody binding to bovine blood cells is present in sera and colostra of BNP donors and is probably initialized by vaccination with a certain BVD vaccine. To understand etiology and pathomechanisms of BNP, we closely characterized disease inducing antibodies regarding immunoglobulin subclass and binding specificities to peripheral blood derived leukocytes and platelets. By exact phenotyping the targeted blood cell subsets, including platelets for the first time, we investigated that BNP alloantibodies are exclusively of IgG1 subclass. Interestingly, IgG1 of BNP colostra bound to 70% leukocytes and 100% platelets irrespective of different bovine breeds and cellular maturity of all specimens tested. Furthermore, staining pattern on platelets as well as leukocyte subsets by BNP-IgG1 alloantibody exposed 100% reactivity to platelets, granulocytes and monocytes. Interestingly, the main part of T-helper cells was not bound by colostral alloantibodies. Our results point to a crucial role of IgG1 antibodies in BNP and to a target antigen that is expressed by all cells of myeloid lineage, but only partially by the lymphoid lineage. PMID:22554492

  10. ALTERATION OF G1-CYCLINS (D1 AND E) IN TRANSITIONAL CELLCARCINOMA OF HUMAN URINARY BLADDER WITH INFECTION OF HPV-18

    Institute of Scientific and Technical Information of China (English)

    郑闪; 何祖根; 刘海涛; 王顺宝

    2002-01-01

    Objective: This study was designed to investigate differential pattern of G1-cyclins (D1 and E) in transitional cell carcinoma (TCC) of human urinary bladder with or without human papillomavirus-18 (HPV-18) infection. Methods: Immunohistochemistry method was used in the detection of the expression of G1-cyclins in 57 cases of TCC (7 normal bladders as control), and HPV-18 DNA was found in 29 cases by polymerases chain reaction (PCR). Results: Cyclin D1 expression was found in 41 of 57 (71.93%) TCCs and it was reverse associated with HPV (x2=8.21, P0.05). Cyclin E expression was found in 17 of 29 (56.82%) in HPV-18 infection group and 19 of 28 (67.86%) in non-HPV infection group. There was obvious difference in the cyclin D1 and cyclin E expression between the TCC and normal tissue (x2=7.46, P<0.05; x2=7.45, P<0.05, respectively). Conclusion: These data demonstrated that HPV infection altered the control of G1 cell cycle. And changes of G1 cell cycle regulatory proteins, either by interaction of cellular protein with viral oncoproteins or by changes in the cellular proteins themselves, may be critical for carcinogenesis of TCC of urinary bladder.

  11. The Cln3 cyclin is down-regulated by translational repression and degradation during the G1 arrest caused by nitrogen deprivation in budding yeast.

    Science.gov (United States)

    Gallego, C; Garí, E; Colomina, N; Herrero, E; Aldea, M

    1997-12-01

    Nutrients are among the most important trophic factors in all organisms. When deprived of essential nutrients, yeast cells use accumulated reserves to complete the current cycle and arrest in the following G1 phase. We show here that the Cln3 cyclin, which has a key role in the timely activation of SBF (Swi4-Swi6)- and MBF (Mbp1-Swi6)-dependent promoters in late G1, is down-regulated rapidly at a post-transcriptional level in cells deprived of the nitrogen source. In addition to the fact that Cln3 is degraded faster by ubiquitin-dependent mechanisms, we have found that translation of the CLN3 mRNA is repressed approximately 8-fold under nitrogen deprivation conditions. As a consequence, both SBF- and MBF-dependent expression is strongly down-regulated. Mainly because of their transcriptional dependence on SBF, and perhaps with the contribution of similar post-transcriptional mechanisms to those found for Cln3, the G1 cyclins Cln1 and 2 become undetectable in starved cells. The complete loss of Cln cyclins and the sustained presence of the Clb-cyclin kinase inhibitor Sic1 in starved cells may provide the molecular basis for the G1 arrest caused by nitrogen deprivation.

  12. The Pseudomonas syringae Type III Effector HopG1 Induces Actin Remodeling to Promote Symptom Development and Susceptibility during Infection.

    Science.gov (United States)

    Shimono, Masaki; Lu, Yi-Ju; Porter, Katie; Kvitko, Brian H; Henty-Ridilla, Jessica; Creason, Allison; He, Sheng Yang; Chang, Jeff H; Staiger, Christopher J; Day, Brad

    2016-07-01

    The plant cytoskeleton underpins the function of a multitude of cellular mechanisms, including those associated with developmental- and stress-associated signaling processes. In recent years, the actin cytoskeleton has been demonstrated to play a key role in plant immune signaling, including a recent demonstration that pathogens target actin filaments to block plant defense and immunity. Herein, we quantified spatial changes in host actin filament organization after infection with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), demonstrating that the type-III effector HopG1 is required for pathogen-induced changes to actin filament architecture and host disease symptom development during infection. Using a suite of pathogen effector deletion constructs, coupled with high-resolution microscopy, we found that deletion of hopG1 from Pst DC3000 resulted in a reduction in actin bundling and a concomitant increase in the density of filament arrays in Arabidopsis, both of which correlate with host disease symptom development. As a mechanism underpinning this activity, we further show that the HopG1 effector interacts with an Arabidopsis mitochondrial-localized kinesin motor protein. Kinesin mutant plants show reduced disease symptoms after pathogen infection, which can be complemented by actin-modifying agents. In total, our results support a model in which HopG1 induces changes in the organization of the actin cytoskeleton as part of its virulence function in promoting disease symptom development. PMID:27217495

  13. First detection of lead in black paper from intraoral film

    International Nuclear Information System (INIS)

    Lead (Pb) contamination in the black paper that recovers intraoral films (BKP) has been investigated. BKP samples were collected from the Radiology Clinics of the Dental School of Ribeirao Preto, University of Sao Paulo, Brazil. For sake of comparison, four different methods were used. The results revealed the presence of high lead levels, well above the maximum limit allowed by the legislation. Pb contamination levels achieved after the following treatments: paper digestion in nitric acid, microwave treatment, DIN38414-54 method and TCLP method were 997 μg g-1, 189 μg g-1, 20.8 μg g-1, and 54.0 μg g-1, respectively. Flame atomic absorption spectrometry (FAAS) and inductively coupled plasma mass spectrometry (ICP-MS) were employed for lead determination according to the protocols of the applied methods. Lead contamination in used BKP was confirmed by scanning electron microscopy coupled with energy dispersive X-ray microanalysis (SEM-EDS). All the SEM imaging was carried out in the secondary electron mode (SE) and backscattered-electron mode (QBSD) following punctual X-ray fluorescence spectra. Soil contamination derived from this product revealed the urgent need of addressing this problem. These elevated Pb levels, show that a preliminary treatment of BKP is mandatory before it is disposed into the common trash. The high lead content of this material makes its direct dumping into the environment unwise.

  14. 14 CFR Appendix F to Part 151 - Appendix F to Part 151

    Science.gov (United States)

    2010-01-01

    ...) AIRPORTS FEDERAL AID TO AIRPORTS Pt. 151, App. F Appendix F to Part 151 There is set forth below an.... Transformer or generator vaults. 11. Control panels for field lighting. 12. Control equipment for...

  15. Calculation of the heavy-hadron axial couplings g1, g2, and g3 using lattice QCD

    Energy Technology Data Exchange (ETDEWEB)

    Will Detmold, David Lin, Stefan Meinel

    2012-06-01

    In a recent paper [arXiv:1109.2480] we have reported on a lattice QCD calculation of the heavy-hadron axial couplings g{sub 1}, g{sub 2}, and g{sub 3}. These quantities are low-energy constants of heavy-hadron chiral perturbation theory (HH{chi}PT) and are related to the B*B{pi}, {Sigma}{sub b}*{Sigma}{sub b}{pi}, and {Sigma}{sub b}{sup (*)}{Lambda}{sub b}{pi} couplings. In the following, we discuss important details of the calculation and give further results. To determine the axial couplings, we explicitly match the matrix elements of the axial current in QCD with the corresponding matrix elements in HH{chi}PT. We construct the ratios of correlation functions used to calculate the matrix elements in lattice QCD, and study the contributions from excited states. We present the complete numerical results and discuss the data analysis in depth. In particular, we demonstrate the convergence of SU(4|2) HH{chi}PT for the axial current matrix elements at pion masses up to about 400 MeV and show the impact of the nonanalytic loop contributions. Finally, we present additional predictions for strong and radiative decay widths of charm and bottom baryons.

  16. PAXX and XLF DNA repair factors are functionally redundant in joining DNA breaks in a G1-arrested progenitor B-cell line.

    Science.gov (United States)

    Kumar, Vipul; Alt, Frederick W; Frock, Richard L

    2016-09-20

    Classical nonhomologous end joining (C-NHEJ) is a major mammalian DNA double-strand break (DSB) repair pathway. Core C-NHEJ factors, such as XRCC4, are required for joining DSB intermediates of the G1 phase-specific V(D)J recombination reaction in progenitor lymphocytes. Core factors also contribute to joining DSBs in cycling mature B-lineage cells, including DSBs generated during antibody class switch recombination (CSR) and DSBs generated by ionizing radiation. The XRCC4-like-factor (XLF) C-NHEJ protein is dispensable for V(D)J recombination in normal cells, but because of functional redundancy, it is absolutely required for this process in cells deficient for the ataxia telangiectasia-mutated (ATM) DSB response factor. The recently identified paralogue of XRCC4 and XLF (PAXX) factor has homology to these two proteins and variably contributes to ionizing radiation-induced DSB repair in human and chicken cells. We now report that PAXX is dispensable for joining V(D)J recombination DSBs in G1-arrested mouse pro-B-cell lines, dispensable for joining CSR-associated DSBs in a cycling mouse B-cell line, and dispensable for normal ionizing radiation resistance in both G1-arrested and cycling pro-B lines. However, we find that combined deficiency for PAXX and XLF in G1-arrested pro-B lines abrogates DSB joining during V(D)J recombination and sensitizes the cells to ionizing radiation exposure. Thus, PAXX provides core C-NHEJ factor-associated functions in the absence of XLF and vice versa in G1-arrested pro-B-cell lines. Finally, we also find that PAXX deficiency has no impact on V(D)J recombination DSB joining in ATM-deficient pro-B lines. We discuss implications of these findings with respect to potential PAXX and XLF functions in C-NHEJ. PMID:27601633

  17. Grays Harbor Paper

    Energy Technology Data Exchange (ETDEWEB)

    Quigg, B. [Grays Harbor Paper, Hoquiam, WA (United States)

    2009-07-01

    Wood waste biomass boilers are used at Grays Harbor Paper in Hoquiam, Washington. This presentation showed that large volumes of biomass are left after a traditional clearcut. The opportunities and challenges of collecting branches, tops and stumps from this wet coastal climate were outlined. The paper described some of the low-tech methods for picking up branches, stumps and woody debris. It included several photographs of custom logging machines for timber harvest, including a brush grapple slasher, a shearer shovel, chippers, grinders, slicesaws, trucks, trailers and caterpillar log loaders for handling slash. The slash recovery program relies on innovative harvesting machines that convert scattered logging slash into bundles that can be easily collected, transported, and stored for use in existing facilities that utilize wood fiber for fuel. figs.

  18. Abstracts of Selected Papers

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    On the Social Solidarity of Organization An Empirical Analysis Li Hanlin Abstract: Based on the 2002 survey data, this paper tries to measure solidarity in organization. The operationalization for this measurement goes from two points of view. One is from the degree of cohesion and another one is from the degree of vulnerability. To observe and measure the degree of cohesion three subscales like social support, vertical integration and organizational identity have been used. To observe and measure the degree of vulnerability other three subscales like dissatisfaction, relative deprivation and anomie have been used. The paper tries to explore finally under which condition the organization behavior and behavior orientation could go to the similarity or make some difference. Key words: Organization Cohesion Vulnerability Organization Behavior

  19. Working Paper 114

    OpenAIRE

    Battilossi, Stefano

    2006-01-01

    What determined the multinational expansion of European banks in the pre-1914 era of globalization? And how were banks’ foreign investments related to other facets of the globalizing world economy such as trade and capital flows? The paper reviews both the contemporary and historical literature, and empirically investigates these issues by using an original panel data based on a sample of more than 50 countries. The dependent variable, aiming at measuring the intensity of cross-border activ...

  20. Solar '94: Technical papers

    International Nuclear Information System (INIS)

    The Solar 94 Conference of the American Solar Energy Society met in San Jose, California to provide a forum for state-of-the-art work in all the solar technologies. The following topics were included in the proceedings: Photovoltaic Modules and Systems; Wind Energy; Solar Thermal Systems; Utility Programs; Solar Water Heating; Solar Fuels; Resource Assessment; Economics and Education. A total of 83 papers were indexed separately for the data base

  1. IDEA papers no 7

    International Nuclear Information System (INIS)

    The Information network on the Economic Development in Aquitaine (IDEA) aims to collect and spread the environmental information concerning the Aquitaine, in order to implement an observatory of the regional environment and of the sustainable development. The IDEA paper no. 7 is devoted to the water quality and management in Gironde. The european framework directive on water and the humid zones are discussed. (A.L.B.)

  2. Working Paper 79

    OpenAIRE

    Elsinger, Helmut; Lehar, Alfred; Summer, Martin

    2002-01-01

    In this paper we suggest a new approach to risk assessment for banks. Rather than looking at them individually we try to undertake an analysis at the level of the banking system. Such a perspective is necessary because the complicated network of mutual credit obligations can make the actual risk exposure of banks invisible at the level of individual institutions. We apply our framework to a cross section of individual bank data as they are usually collected at the central bank. Using standard...

  3. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in

  4. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in mainland China that has an international readership in mind.

  5. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in Mainland China that has an international

  6. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in mainland

  7. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in mainland

  8. Iceland; Selected Issues Paper

    OpenAIRE

    International Monetary Fund

    2012-01-01

    This Selected Issues paper examines implications of capital account liberalization in Iceland. Capital controls were critical in 2008 to avoid a more severe collapse of the Icelandic economy. Six years later, capital inflows have been liberalized, but most outflows remain restricted. Iceland has used the breathing room to reduce flow and stock vulnerabilities, strengthen institutions, and prepare for the lifting of capital controls. Simulations using the central bank’s Quarterly Macroeconom...

  9. Corrosion/94 conference papers

    International Nuclear Information System (INIS)

    The approximately 500 papers from this conference are divided into the following sections: Rail transit systems--stray current corrosion problems and control; Total quality in the coatings industry; Deterioration mechanisms of alloys at high temperatures--prevention and remediation; Research needs and new developments in oxygen scavengers; Computers in corrosion control--knowledge based system; Corrosion and corrosivity sensors; Corrosion and corrosion control of steel reinforced concrete structures; Microbiologically influenced corrosion; Practical applications in mitigating CO2 corrosion; Mineral scale deposit control in oilfield-related operations; Corrosion of materials in nuclear systems; Testing nonmetallics for life prediction; Refinery industry corrosion; Underground corrosion control; Mechanisms and applications of deposit and scale control additives; Corrosion in power transmission and distribution systems; Corrosion inhibitor testing and field application in oil and gas systems; Decontamination technology; Ozone in cooling water applications, testing, and mechanisms; Corrosion of water and sewage treatment, collection, and distribution systems; Environmental cracking of materials; Metallurgy of oil and gas field equipment; Corrosion measurement technology; Duplex stainless steels in the chemical process industries; Corrosion in the pulp and paper industry; Advances in cooling water treatment; Marine corrosion; Performance of materials in environments applicable to fossil energy systems; Environmental degradation of and methods of protection for military and aerospace materials; Rail equipment corrosion; Cathodic protection in natural waters; Characterization of air pollution control system environments; and Deposit-related problems in industrial boilers. Papers have been processed separately for inclusion on the data base

  10. Variation of Surface Temperature during the Last Millennium in a Simulation with the FGOALS-g1 Climate System Model

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jie; Laurent LI; ZHOU Tianjun; XIN Xiaoge

    2013-01-01

    A reasonable past millennial climate simulation relies heavily on the specified external forcings,including both natural and anthropogenic forcing agents.In this paper,we examine the surface temperature responses to specified external forcing agents in a millennium-scale transient climate simulation with the fast version of LASG IAP Flexible Global Ocean-Atmosphere-Land System model (FGOALS-gl) developed in the State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics,Institute of Atmospheric Physics (LASG/IAP).The model presents a reasonable performance in comparison with reconstructions of surface temperature.Differentiated from significant changes in the 20th century at the global scale,changes during the natural-forcing-dominant period are mainly manifested in the Northern Hemisphere.Seasonally,modeled significant changes are more pronounced during the wintertime at higher latitudes.This may be a manifestation of polar amplification associated with sea-ice-temperature positive feedback.The climate responses to total external forcings can explain about half of the climate variance during the whole millennium period,especially at decadal timescales.Surface temperature in the Antarctic shows heterogeneous and insignificant changes during the preindustrial period and the climate response to external forcings is undetectable due to the strong internal variability.The model response to specified external forcings is modulated by cloud radiative forcing (CRF).The CRF acts against the fluctuations of external forcings.Effects of clouds are manifested in shortwave radiation by changes in cloud water during the natural-forcing-dominant period,but mainly in longwave radiation by a decrease in cloud amount in the anthropogenic-forcing-dominant period.

  11. Analysis of Gene Evolution, Protein Expression and Identification of Echinococcus granulosus EgG1Y162%细粒棘球蚴EgG1Y162基因进化分析、表达及鉴定

    Institute of Scientific and Technical Information of China (English)

    祖力皮也·吐尔逊; 曹春宝; 温浩; 丁剑冰; 德力夏提·依米提

    2016-01-01

    目的:克隆和鉴定细粒棘球蚴的EgG1 Y162基因,分析其蛋白表达和适应性进化并鉴定抗原性.方法:根据emY162基因序列设计引物,分别从细粒棘球绦虫原头蚴、囊壁生发层、成虫和虫卵四个发育阶段,提取基因组DNA和总RNA,mRNA反转录为cDNA,利用PCR的方法以基因组DNA和cDNA为模板扩增EgG1Y162基因;构建PUCm-T/EgG1Y162重组质粒,经PCR、酶切及测序鉴定后,测序确定其正确性.利用DNAman软件与MEGA4软件对EgG1Y162基因特点进行分析并构建EgG1Y162核酸序列的进化树进一步探讨其同源性.荧光定量PCR检测EgG1Y162基因在细粒棘球绦虫原头蚴、囊壁生发层、成虫和虫卵四个不同发育阶段的表达情况.利用定向克隆技术将EgG1Y162抗原基因片段克隆至原核表达质粒PET-41a上,通过酶切分析和PCR鉴定筛选出阳性克隆,测序确定序列.IPTG初步诱导和表达EgG1Y162-GST重组蛋白,通过SDS-PAGE电泳和Westernblot试验分析鉴定.结果:从细粒棘球绦虫的两个不同发育阶段均克隆出EgG1Y162基因,从总DNA克隆得到片段长度1 680bp,从cDNA克隆得到片段长度459bp.相似性比较表明,EgG1Y162基因序列与emY162相似性为91%,而EgG1Y162基因cDNA与emY162相似性为95%.进一步分析显示,EgG1Y162基因序列由3个外显子和2个内含子组成,外显子区域分别为1~70,1064~1 380和1 577 ~1 648.位于疏水端1~16位氨基酸构成EgG1Y162信号肽序列,35~ 115位氨基酸形成一个大的纤黏连蛋白剪接体FN3,133 ~ 152位氨基酸构成羧基端跨膜区域.测序结果显示EgG1Y162抗原基因长度为360bp,编码120个氨基酸.通过荧光定量PCR检测,发现EgG1Y162在成虫、生发层阶段、原头蚴和虫卵阶段均有不同程度的表达.但是EgG1Y162在成虫中的表达量最多,相对值为19.526,差异有统计学意义(P<0.01),其次生发层阶段,为5.122,再次在原头蚴阶段,相对值为5.083,

  12. Appendix: Business Cycle Implications of Internal Consumption Habit for New Keynesian Models

    OpenAIRE

    Kano, Takashi; James M. Nason

    2012-01-01

    The appendix discusses computational aspects of the paper “Business Cycle Implications of Internal Consumption Habit for New Keynesian Models.” These topics range from solving the baseline new Keynesian dynamic stochastic general equilibrium (NKDSGE) model, estimating the structural infinite-order vector moving averages, checking whether these models recover the fundamental shocks, to computing the permanent and transitory output and consumption growth spectral densities. More evidence about ...

  13. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in Mainland China that has an international readership in mind.This journal is aimed to establish a platform for LIS students,educators,researchers and library practitioners all over the world to engage in

  14. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in mainland China that has an international readership in mind.This journal is aimed to establish a platform for LIS students,educators,researchers and library practitioners all over the world to engage in

  15. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in mainland China that has an international readership in mind.This journal is aimed to establish a platform for LIS students,educators,researchers and library practitioners

  16. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    <正>Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in mainland China that has an international readership in mind.This journal is aimed to establish a platform for LIS students,educators,researchers and library practitioners all over the world to engage in

  17. Corrosion/95 conference papers

    International Nuclear Information System (INIS)

    The papers in this conference represent the latest technological advances in corrosion control and prevention. The following subject areas are covered: cathodic protection in natural waters; materials for fossil fuel combustion and conversion systems; modern problems in atmospheric corrosion; innovative ideas for controlling the decaying infrastructure; deposits and their effects on corrosion in industry; volatile high temperature and non aqueous corrosion inhibitors; corrosion of light-weight and precoated metals for automotive application; refining industry corrosion; corrosion in pulp and paper industry; arctic/cold weather corrosion; materials selection for waste incinerators and associated equipment; corrosion measurement technology; environmental cracking of materials; advancing technology in the coating industry; corrosion in gas treating; green inhibition; recent advances in corrosion control of rail equipment; velocity effects and erosion corrosion in oil and gas production; marine corrosion; corrosion of materials in nuclear systems; underground corrosion control; corrosion in potable and industrial water systems in buildings and its impact on environmental compliance; deposit related boiler tube failures; boiler systems monitoring and control; recent developments and experiences in reactive metals; microbiologically influenced corrosion; corrosion and corrosion control for steel reinforced concrete; international symposium on the use of 12 and 13 Cr stainless steels in oil and gas production environments; subsea corrosion /erosion monitoring in production facilities; fiberglass reinforced pipe and tubulars in oilfield service; corrosion control technology in power transmission and distribution; mechanisms and methods of scale and deposit control; closing the loop -- results oriented cooling system monitoring and control; and minimization of aqueous discharge

  18. List of working papers

    International Nuclear Information System (INIS)

    The following working papers are included in this report. The other papers, or the memos of which they consist, are available from the IAEA Nuclear Data Section. WP2001-2 Inventory of TRANS files exchanged since the last meeting; WP2001-4 Data headings and units for wavelength and kT; WP2001-6 Proposed Polarization Quantities; WP2001-7 Correlation / Angular correlation: Clarifications and dictionary cleanup; WP2001-8 Proposed quantity PAR/M-,DA,G; WP2001-14 Units N/PART/SR etc. for Dict. 25; WP2001-15 Coding of differential neutron multiplicity distributions; WP2001-16 Headings E-LVL-INI, E-LVL-FIN as 'additional information'; WP2001-17 Dictionary sorting flags and wildcards; WP2001-18 IAEA/NDS priorities in the EXFOR compilation; WP2001-21 CSISRS Library Statistics (NNDC); WP2001-23 CINDA-2001 Manual (24 May 2001); WP2001-24 Correspondence of quantity codes for CINDA-2001; WP2001-25 EXFOR as a multi-platform relational database: current status of development; WP2001-26 Compilation and Evaluation of Alpha-Induced Nuclear Reaction Cross Sections for Astrophysics; WP2001-28 Journal coverage for CINDA

  19. The environmental survey manual: Appendix D

    International Nuclear Information System (INIS)

    The purpose of this manual is to provide guidance to the Survey and Sampling and Analysis teams that conduct the one-time Environmental Survey of the major US Department of Energy operating facilities. This appendix contains procedures for chemical analysis of organics, inorganics, and radioisotopes

  20. Energy 21. Preconditions and results. Appendix

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    This appendix comprises the following 5 scenarios: Reference scenario for electricity and district heating supply; Action plan scenario for electricity and district heat supply; Energy balances for reference and action plan scenarios; Investments and operating costs for user installations; Projection of CO{sub 2} emissions from the transport sector. (EG)

  1. Characterization of Paper Heterogeneity

    Science.gov (United States)

    Considine, John M.

    Paper and paperboard are the most widely-used green materials in the world because they are renewable, recyclable, reusable, and compostable. Continued and expanded use of these materials and their potential use in new products requires a comprehensive understanding of the variability of their mechanical properties. This work develops new methods to characterize the mechanical properties of heterogeneous materials through a combination of techniques in experimental mechanics, materials science and numerical analysis. Current methods to analyze heterogeneous materials focus on crystalline materials or polymer-crystalline composites, where material boundaries are usually distinct. This work creates a methodology to analyze small, continuously-varying stiffness gradients in 100% polymer systems and is especially relevant to paper materials where factors influencing heterogeneity include local mass, fiber orientation, individual pulp fiber properties, local density, and drying restraint. A unique approach was used to understand the effect of heterogeneity on paper tensile strength. Additional variation was intentionally introduced, in the form of different size holes, and their effect on strength was measured. By modifying two strength criteria, an estimate of strength in the absence of heterogeneity was determined. In order to characterize stiffness heterogeneity, a novel load fixture was developed to excite full-field normal and shear strains for anisotropic stiffness determination. Surface strains were measured with digital image correlation and were analyzed with the VFM (Virtual Fields Method). This approach led to VFM-identified stiffnesses that were similar to values determined by conventional tests. The load fixture and VFM analyses were used to measure local stiffness and local stiffness variation on heterogeneous anisotropic materials. The approach was validated on simulated heterogeneous materials and was applied experimentally to three different paperboards

  2. Call for papers

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Chinese Journal of Library and Information Science invites submission of academic papers from library and information science practitioners,educators and researchers.This peer-reviewed academic journal in the subject field of Library and Information Science(LIS)is published quarterly under the aegis of the National Science Library,Chinese Academy of Sciences.The Journal is the first English-language academic journal in LIS ever published in mainland China that has an international readership in mind.This journal is aimed to establish a platform for LIS students,educators,researchers and library practitioners all over the world to engage in intellectual dialogue so as to upgrade continuously library practice around the world in general and that of China in particular.

  3. 一类N-策略M/G/1排队系统队长分布%The Queue Length Distribution of Queueing System under N-Policy

    Institute of Scientific and Technical Information of China (English)

    刘晓燕; 孙玺菁; 刘丹

    2013-01-01

    In this paper, a new method was developedfor calculating the queue length distribution on one M/G/1 queueing system under N-policy with general startup/closedown. Firstly, the stochastic decomposition property was used to derive the p.g.f. of the queue length distribution. By the Leibniz formula of derivation, the addition⁃al queue length distribution was investigated, and then, the expression of queue length distribution was derived. Finally, an example was given for illustrating the application of the expression.%  引入一种新的分析方法,研究了一类具有启动/关闭时间的N-策略的M/G/1排队系统的队长分布。首先,使用随机分解技术导出了系统的队长分布的概率母函数;再根据Leibniz公式结合随机分解结果直接得到附加队长分布,进一步得到该排队系统队长分布的表达式;最后,举例说明队长分布表达式的分析应用。

  4. Detection of IgG1 antibodies against Mycobacterium tuberculosis DosR and Rpf antigens in tuberculosis patients before and after chemotherapy.

    Science.gov (United States)

    Mattos, Ana Márcia Menezes; Chaves, Alexandre Silva; Franken, Kees L M C; Figueiredo, Bárbara Bruna Muniz; Ferreira, Ana Paula; Ottenhoff, Tom H M; Teixeira, Henrique Couto

    2016-01-01

    Diagnosis of tuberculosis (TB) remains challenging. Serum IgG1 antibodies against Mycobacterium tuberculosis active growth phase antigens (ESAT-6/CFP-10, Rv0717 and Rv3353), DosR regulon-encoded proteins (Rv1733, Rv1737, Rv2628 and Rv2029), and resuscitation-promoting factors (Rv0867 and Rv2389) were evaluated in TB patients using ELISA. Active TB patients showed elevated levels of IgG1 antibodies against ESAT-6/CFP-10, Rv0717, Rv3353, Rv1733, Rv2628, Rv2029 and Rv0867 in comparison to healthy controls (p tuberculosis antigens, including DosR and Rpf proteins, may represent an additional tool in the diagnosis of tuberculosis.

  5. Generation of Discrete Bicubic G1 B-Spline Ship Hullform Surfaces from a Given Curve Network Using Virtual Iso-Parametric Curves

    Institute of Scientific and Technical Information of China (English)

    Joong-Hyun Rhim; Doo-Yeoun Cho; Kyu-Yeul Lee; Tae-Wan Kim

    2006-01-01

    We propose a method that automatically generates discrete bicubic G1 continuous B-spline surfaces that interpolate the curve network of a ship hullform. First, the curves in the network are classified into two types: boundary curves and "reference curves". The boundary curves correspond to a set of rectangular (or triangular) topological type that can be represented with tensor-product (or degenerate) B-spline surface patches. Next, in the interior of the patches,surface fitting points and cross boundary derivatives are estimated from the reference curves by constructing "virtual" isoparametric curves. Finally, a discrete G1 continuous B-spline surface is generated by a surface fitting algorithm. Several smooth ship hullform surfaces generated from curve networks corresponding to actual ship hullforms demonstrate the quality of the method.

  6. Suppression of the p53- or pRB-mediated G1 checkpoint is required for E2F-induced S-phase entry

    DEFF Research Database (Denmark)

    Lomazzi, Marina; Moroni, M Cristina; Jensen, Michael R;

    2002-01-01

    Deregulation of the retinoblastoma protein (pRB) pathway is a hallmark of cancer. In the absence of other genetic alterations, this deregulation results in lack of differentiation, hyperproliferation and apoptosis. The pRB protein acts as a transcriptional repressor by targeting the E2F...... fibroblasts, increased E2F1 activity can result in S-phase entry in diploid fibroblasts in which the p53-mediated G1 checkpoint is suppressed. In addition, we show that E2F1 can induce S phase in primary mouse fibroblasts lacking pRB. These results indicate that, in addition to acting as an E2F......-dependent transcriptional repressor, pRB is also required for the cells to retain the G1 checkpoint in response to unprogrammed proliferative signals....

  7. Analysis of the APX, PGD1 and R1G1B constitutive gene promoters in various organs over three homozygous generations of transgenic rice plants.

    Science.gov (United States)

    Park, Su-Hyun; Bang, Seung Woon; Jeong, Jin Seo; Jung, Harin; Redillas, Mark Christian Felipe Reveche; Kim, Hyung Il; Lee, Kang Hyun; Kim, Youn Shic; Kim, Ju-Kon

    2012-06-01

    We have previously characterized the constitutively active promoters of the APX, PGD1 and R1G1B genes in rice (Park et al. 2010 in J Exp Bot 61:2459-2467). To have potential crop biotechnology applications, gene promoters must be stably active over many generations. In our current study, we report our further detailed analysis of the APX, PGD1 and R1G1B gene promoters in various organs and tissues of transgenic rice plants for three (T₃₋₅) homozygous generations. The copy numbers in 37 transgenic lines that harbor promoter:gfp constructs were determined and promoter activities were measured by real-time qPCR. Analysis of the 37 lines revealed that 15 contained a single copy of one of the three promoter:gfp chimeric constructs. The promoter activity levels were generally higher in multi-copy lines, whereas variations in these levels over the T₃₋₅ generations studied were observed to be smaller in single-copy than in multi-copy lines. The three promoters were further found to be highly active in the whole plant body at both the vegetative and reproductive stages of plant growth, with the exception of the APX in the ovary and R1G1B in the pistil and filaments where zero or very low levels of activity were detected. Of note, the spatial activities of the PGD1 promoter were found to be strikingly similar to those of the ZmUbi1, a widely used constitutive promoter. Our comparison of promoter activities between T₃, T₄ and T₅ plants revealed that the APX, PGD1 and R1G1B promoters maintained their activities at comparable levels in leaves and roots over three homozygous generations and are therefore potentially viable alternative promoters for crop biotechnology applications.

  8. Draft Genome Sequence of Geobacillus icigianus Strain G1w1T Isolated from Hot Springs in the Valley of Geysers, Kamchatka (Russian Federation).

    Science.gov (United States)

    Bryanskaya, Alla V; Rozanov, Aleksey S; Logacheva, Maria D; Kotenko, Anastasia V; Peltek, Sergey E

    2014-10-23

    The Geobacillus icigianus G1w1(T) strain was isolated from sludge samples of unnamed vaporing hydrothermal (97°С) outlets situated in a geyser in the Troinoy region (Valley of Geysers, Kronotsky Nature Reserve, Kamchatka, Russian Federation; 54°25'51.40″N, 160°7'41.40″E). The sequenced and annotated genome is 3,457,810 bp and encodes 3,342 genes.

  9. Characterization of pH dependent Mn(II) oxidation strategies and formation of a bixbyite-like phase by Mesorhizobium australicum T-G1

    Science.gov (United States)

    Bohu, Tsing; Santelli, Cara M; Akob, Denise M.; Neu, Thomas R; Ciobota, Valerian; Rösch, Petra; Popp, Jürgen; Nietzsche, Sándor; Küsel, Kirsten

    2015-01-01

    Despite the ubiquity of Mn oxides in natural environments, there are only a few observations of biological Mn(II) oxidation at pH < 6. The lack of low pH Mn-oxidizing bacteria (MOB) isolates limits our understanding of how pH influences biological Mn(II) oxidation in extreme environments. Here, we report that a novel MOB isolate, Mesorhizobium australicum strain T-G1, isolated from an acidic and metalliferous uranium mining area, can oxidize Mn(II) at both acidic and neutral pH using different enzymatic pathways. X-ray diffraction, Raman spectroscopy, and scanning electron microscopy with energy dispersive X-ray spectroscopy revealed that T-G1 initiated bixbyite-like Mn oxide formation at pH 5.5 which coincided with multi-copper oxidase expression from early exponential phase to late stationary phase. In contrast, reactive oxygen species (ROS), particularly superoxide, appeared to be more important for T-G1 mediated Mn(II) oxidation at neutral pH. ROS was produced in parallel with the occurrence of Mn(II) oxidation at pH 7.2 from early stationary phase. Solid phase Mn oxides did not precipitate, which is consistent with the presence of a high amount of H2O2 and lower activity of catalase in the liquid culture at pH 7.2. Our results show that M. australicum T-G1, an acid tolerant MOB, can initiate Mn(II) oxidation by varying its oxidation mechanisms depending on the pH and may play an important role in low pH manganese biogeochemical cycling.

  10. Characterization of pH dependent Mn(II) oxidation strategies and formation of a bixbyite-like phase by Mesorhizobium australicum T-G1

    Science.gov (United States)

    Bohu, Tsing; Santelli, Cara M.; Akob, Denise M.; Neu, Thomas R.; Ciobota, Valerian; Rösch, Petra; Popp, Jürgen; Nietzsche, Sándor; Küsel, Kirsten

    2015-01-01

    Despite the ubiquity of Mn oxides in natural environments, there are only a few observations of biological Mn(II) oxidation at pH < 6. The lack of low pH Mn-oxidizing bacteria (MOB) isolates limits our understanding of how pH influences biological Mn(II) oxidation in extreme environments. Here, we report that a novel MOB isolate, Mesorhizobium australicum strain T-G1, isolated from an acidic and metalliferous uranium mining area, can oxidize Mn(II) at both acidic and neutral pH using different enzymatic pathways. X-ray diffraction, Raman spectroscopy, and scanning electron microscopy with energy dispersive X-ray spectroscopy revealed that T-G1 initiated bixbyite-like Mn oxide formation at pH 5.5 which coincided with multi-copper oxidase expression from early exponential phase to late stationary phase. In contrast, reactive oxygen species (ROS), particularly superoxide, appeared to be more important for T-G1 mediated Mn(II) oxidation at neutral pH. ROS was produced in parallel with the occurrence of Mn(II) oxidation at pH 7.2 from early stationary phase. Solid phase Mn oxides did not precipitate, which is consistent with the presence of a high amount of H2O2 and lower activity of catalase in the liquid culture at pH 7.2. Our results show that M. australicum T-G1, an acid tolerant MOB, can initiate Mn(II) oxidation by varying its oxidation mechanisms depending on the pH and may play an important role in low pH manganese biogeochemical cycling. PMID:26236307

  11. Extremely low-frequency electromagnetic fields cause G1 phase arrest through the activation of the ATM-Chk2-p21 pathway.

    Directory of Open Access Journals (Sweden)

    Chao-Ying Huang

    Full Text Available In daily life, humans are exposed to the extremely low-frequency electromagnetic fields (ELF-EMFs generated by electric appliances, and public concern is increasing regarding the biological effects of such exposure. Numerous studies have yielded inconsistent results regarding the biological effects of ELF-EMF exposure. Here we show that ELF-EMFs activate the ATM-Chk2-p21 pathway in HaCaT cells, inhibiting cell proliferation. To present well-founded results, we comprehensively evaluated the biological effects of ELF-EMFs at the transcriptional, protein, and cellular levels. Human HaCaT cells from an immortalized epidermal keratinocyte cell line were exposed to a 1.5 mT, 60 Hz ELF-EMF for 144 h. The ELF-EMF could cause G1 arrest and decrease colony formation. Protein expression experiments revealed that ELF-EMFs induced the activation of the ATM/Chk2 signaling cascades. In addition, the p21 protein, a regulator of cell cycle progression at G1 and G2/M, exhibited a higher level of expression in exposed HaCaT cells compared with the expression of sham-exposed cells. The ELF-EMF-induced G1 arrest was diminished when the CHK2 gene expression (which encodes checkpoint kinase 2; Chk2 was suppressed by specific small interfering RNA (siRNA. These findings indicate that ELF-EMFs activate the ATM-Chk2-p21 pathway in HaCaT cells, resulting in cell cycle arrest at the G1 phase. Based on the precise control of the ELF-EMF exposure and rigorous sham-exposure experiments, all transcriptional, protein, and cellular level experiments consistently supported the conclusion. This is the first study to confirm that a specific pathway is triggered by ELF-EMF exposure.

  12. Synchronization in G0/G1 enhances the mitogenic response of cells overexpressing the human insulin receptor A isoform to insulin

    Science.gov (United States)

    Nelander, Gitte-Mai; Hansen, Bo Falck; Jensen, Pia; Krabbe, Jonas S.; Jensen, Marianne B.; Hegelund, Anne Charlotte; Svendsen, Jette E.; Oleksiewicz, Martin B.

    2009-01-01

    Evaluating mitogenic signaling specifically through the human insulin receptor (IR) is relevant for the preclinical safety assessment of developmental insulin analogs. It is known that overexpression of IR sensitizes cells to the mitogenic effects of insulin, but it is essentially unknown how mitogenic responses can be optimized to allow practical use of such recombinant cell lines for preclinical safety testing. We constitutively overexpressed the short isoform of the human insulin receptor (hIR-A, exon 11-negative) in L6 rat skeletal myoblasts. Because the mitogenic effect of growth factors such as insulin is expected to act in G0/G1, promoting S-phase entry, we developed a combined topoinhibition + serum deprivation strategy to explore the effect of G0/G1 synchronization as an independent parameter in the context of serum deprivation, the latter being routinely used to reduce background in mitogenicity assays. G0/G1 synchronization significantly improved the mitogenic responses of L6-hIR cells to insulin, measured by 3H-thymidine incorporation. Comparison with the parental L6 cells using phospho-mitogen-activated protein kinase, phospho-AKT, as well as 3H-thymidine incorporation end points supported that the majority of the mitogenic effect of insulin in L6-hIR cells was mediated by the overexpressed hIR-A. Using the optimized L6-hIR assay, we found that the X-10 insulin analog was more mitogenic than native human insulin, supporting that X-10 exhibits increased mitogenic signaling through the hIR-A. In summary, this study provides the first demonstration that serum deprivation may not be sufficient, and G0/G1 synchronization may be required to obtain optimal responsiveness of hIR-overexpressing cell lines for preclinical safety testing. PMID:19898946

  13. Understanding the Relationship Between Biotherapeutic Protein Stability and Solid–Liquid Interfacial Shear in Constant Region Mutants of IgG1 and IgG4

    OpenAIRE

    Tavakoli-Keshe, R.; Phillips, J.J.; Turner, R.; Bracewell, D. G.

    2013-01-01

    Relative stability of therapeutic antibody candidates is currently evaluated primarily through their response to thermal degradation, yet this technique is not always predictive of stability in manufacture, shipping, and storage. A rotating disk shear device is proposed that produces defined shear conditions at a known solid–liquid interface to measure stability in this environment. Five variants of IgG1 and IgG4 antibodies were created using combinations of two discrete triple amino acid seq...

  14. Characterization of pH dependent Mn(II oxidation strategies and formation of a bixbyite-like phase by Mesorhizobium australicum T-G1

    Directory of Open Access Journals (Sweden)

    Tsing eBohu

    2015-07-01

    Full Text Available Despite the ubiquity of Mn oxides in natural environments, there are only a few observations of biological Mn(II oxidation at pH < 6. The lack of low pH Mn-oxidizing bacteria (MOB isolates limits our understanding of how pH influences biological Mn(II oxidation in extreme environments. Here, we report that a novel MOB isolate, Mesorhizobium australicum strain T-G1, isolated from an acidic and metalliferous uranium mining area, can oxidize Mn(II at both acidic and neutral pH using different enzymatic pathways. X-ray diffraction (XRD, Raman spectroscopy, and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDS revealed that T-G1 initiated bixbyite-like Mn oxide formation at pH 5.5 which coincided with multi-copper oxidase (MCO expression from early exponential phase to late stationary phase. In contrast, reactive oxygen species (ROS, particularly superoxide, appeared to be more important for T-G1 mediated Mn(II oxidation at neutral pH. ROS was produced in parallel with the occurrence of Mn(II oxidation at pH 7.2 from early stationary phase. Solid phase Mn oxides did not precipitate, which is consistent with the presence of a high amount of H2O2 and lower activity of catalase in the liquid culture at pH 7.2. Our results show that M. australicum T-G1, an acid tolerant MOB, can initiate Mn(II oxidation by varying its oxidation mechanisms depending on the pH and may play an important role in low pH manganese biogeochemical cycling.

  15. Genomic and Transcriptomic Associations Identify a New Insecticide Resistance Phenotype for the Selective Sweep at the Cyp6g1 Locus of Drosophila melanogaster.

    Science.gov (United States)

    Battlay, Paul; Schmidt, Joshua M; Fournier-Level, Alexandre; Robin, Charles

    2016-01-01

    Scans of the Drosophila melanogaster genome have identified organophosphate resistance loci among those with the most pronounced signature of positive selection. In this study, the molecular basis of resistance to the organophosphate insecticide azinphos-methyl was investigated using the Drosophila Genetic Reference Panel, and genome-wide association. Recently released full transcriptome data were used to extend the utility of the Drosophila Genetic Reference Panel resource beyond traditional genome-wide association studies to allow systems genetics analyses of phenotypes. We found that both genomic and transcriptomic associations independently identified Cyp6g1, a gene involved in resistance to DDT and neonicotinoid insecticides, as the top candidate for azinphos-methyl resistance. This was verified by transgenically overexpressing Cyp6g1 using natural regulatory elements from a resistant allele, resulting in a 6.5-fold increase in resistance. We also identified four novel candidate genes associated with azinphos-methyl resistance, all of which are involved in either regulation of fat storage, or nervous system development. In Cyp6g1, we find a demonstrable resistance locus, a verification that transcriptome data can be used to identify variants associated with insecticide resistance, and an overlap between peaks of a genome-wide association study, and a genome-wide selective sweep analysis. PMID:27317781

  16. Importance of trivalency and the e(g)(1) configuration in the photocatalytic oxidation of water by Mn and Co oxides.

    Science.gov (United States)

    Maitra, Urmimala; Naidu, B S; Govindaraj, A; Rao, C N R

    2013-07-16

    Prompted by the early results on the catalytic activity of LiMn2O4 and related oxides in the photochemical oxidation of water, our detailed study of several manganese oxides has shown that trivalency of Mn is an important factor in determining the catalytic activity. Thus, Mn2O3, LaMnO3, and MgMn2O4 are found to be very good catalysts with turnover frequencies of 5 × 10(-4) s(-1), 4.8 × 10(-4) s(-1), and 0.8 × 10(-4) s(-1), respectively. Among the cobalt oxides, Li2Co2O4 and LaCoO3--especially the latter--exhibit excellent catalytic activity, with the turnover frequencies being 9 × 10(-4) s(-1) and 1.4 × 10(-3) s(-1), respectively. The common feature among the catalytic Mn and Co oxides is not only that Mn and Co are in the trivalent state, but Co(3+) in the Co oxides is in the intermediate t2g(5)e(g)(1) state whereas Mn(3+) is in the t2g(3e(g)(1) state. The presence of the e(g)(1) electron in these Mn and Co oxides is considered to play a crucial role in the photocatalytic properties of the oxides.

  17. PP2A-mediated regulation of Ras signaling in G2 is essential for stable quiescence and normal G1 length.

    Science.gov (United States)

    Naetar, Nana; Soundarapandian, Velmurugan; Litovchick, Larisa; Goguen, Kelsey L; Sablina, Anna A; Bowman-Colin, Christian; Sicinski, Piotr; Hahn, William C; DeCaprio, James A; Livingston, David M

    2014-06-19

    Quiescence (G0) allows cycling cells to reversibly cease proliferation. A decision to enter quiescence is suspected of occurring early in G1, before the restriction point (R). Surprisingly, we have identified G2 as an interval during which inhibition of the protein phosphatase PP2A results in failure to exhibit stable quiescence. This effect is accompanied by shortening of the ensuing G1. The PP2A subcomplex required for stable G0 contains the B56γ B subunit. After PP2A inhibition in G2, aberrant overexpression of cyclin E occurs during mitosis and is responsible for overriding quiescence. Strikingly, suppression of Ras signaling re-establishes normal cyclin E levels during M and restores G0. These data point to PP2A-B56γ-driven Ras signaling modulation in G2 as essential for suppressing aberrant cyclin E expression during mitosis and thereby achieving normal G0 control. Thus, G2 is an interval during which the length and growth factor dependence of the next G1 interval are established. PMID:24857551

  18. PP2A-mediated regulation of Ras signaling in G2 is essential for stable quiescence and normal G1 length

    Science.gov (United States)

    Naetar, Nana; Soundarapandian, Velmurugan; Litovchick, Larisa; Goguen, Kelsey L.; Sablina, Anna A.; Bowman-Colin, Christian; Sicinski, Piotr; Hahn, William C.; DeCaprio, James A.; Livingston, David M.

    2014-01-01

    SUMMARY Quiescence (G0) allows cycling cells to reversibly cease proliferation. A decision to enter quiescence is suspected of occurring early in G1, before the restriction point, R. Surprisingly, we have identified G2 as an interval during which inhibition of the protein phosphatase, PP2A, results in failure to exhibit stable quiescence. This effect is accompanied by shortening of the ensuing G1. The PP2A subcomplex required for stable G0 contains the B56γ B subunit. Following PP2A inhibition in G2, aberrant overexpression of cyclin E occurs during mitosis and is responsible for overriding quiescence. Strikingly, suppression of Ras signaling re-establishes normal cyclin E levels during M and restores G0. These data point to PP2A-B56γ-driven Ras signaling-modulation in G2 as essential for suppressing aberrant cyclin E expression during mitosis and, thereby, achieving normal G0 control. Thus, G2 is an interval during which the length and growth factor dependence of the next G1 interval are established. PMID:24857551

  19. Effects of Protein Conformation, Apparent Solubility, and Protein-Protein Interactions on the Rates and Mechanisms of Aggregation for an IgG1Monoclonal Antibody.

    Science.gov (United States)

    Kalonia, Cavan; Toprani, Vishal; Toth, Ronald; Wahome, Newton; Gabel, Ian; Middaugh, C Russell; Volkin, David B

    2016-07-28

    Non-native protein aggregation is a key degradation pathway of immunoglobulins. In this work, the aggregation kinetics of an immunoglobulin gamma-1 monoclonal antibody (IgG1 mAb) in different solution environments was monitored over a range of incubation temperatures for up to seven months using size exclusion chromatography. Histidine and citrate buffers with/without sodium chloride were employed to modulate the mAb's conformational stability, solubility (in the presence of polyethylene glycol, PEG), and protein-protein interactions as measured by differential scanning calorimetry, PEG precipitation, and static light scattering, respectively. The effect of these parameters on the mechanism(s) of mAb aggregation during storage at different temperatures was determined using kinetic models, which were used to fit aggregation data to determine rate constants for aggregate nucleation and growth processes. This approach was used to investigate the effects of colloidal protein-protein interactions and solubility values (in PEG solutions) on the mechanisms and rates of IgG1 mAb aggregation as a function of temperature-induced structural perturbations. Aggregate nucleation and growth pathways for this IgG1 mAb were sensitive to temperature and overall conformational stability. Aggregate growth, on the other hand, was also sensitive to conditions affecting the solubility of the mAb, particularly at elevated temperatures. PMID:27380437

  20. Orc1 Binding to Mitotic Chromosomes Precedes Spatial Patterning during G1 Phase and Assembly of the Origin Recognition Complex in Human Cells.

    Science.gov (United States)

    Kara, Nihan; Hossain, Manzar; Prasanth, Supriya G; Stillman, Bruce

    2015-05-01

    Replication of eukaryotic chromosomes occurs once every cell division cycle in normal cells and is a tightly controlled process that ensures complete genome duplication. The origin recognition complex (ORC) plays a key role during the initiation of DNA replication. In human cells, the level of Orc1, the largest subunit of ORC, is regulated during the cell division cycle, and thus ORC is a dynamic complex. Upon S phase entry, Orc1 is ubiquitinated and targeted for destruction, with subsequent dissociation of ORC from chromosomes. Time lapse and live cell images of human cells expressing fluorescently tagged Orc1 show that Orc1 re-localizes to condensing chromatin during early mitosis and then displays different nuclear localization patterns at different times during G1 phase, remaining associated with late replicating regions of the genome in late G1 phase. The initial binding of Orc1 to mitotic chromosomes requires C-terminal amino acid sequences that are similar to mitotic chromosome-binding sequences in the transcriptional pioneer protein FOXA1. Depletion of Orc1 causes concomitant loss of the mini-chromosome maintenance (Mcm2-7) helicase proteins on chromatin. The data suggest that Orc1 acts as a nucleating center for ORC assembly and then pre-replication complex assembly by binding to mitotic chromosomes, followed by gradual removal from chromatin during the G1 phase. PMID:25784553

  1. NBM-T-BBX-OS01, Semisynthesized from Osthole, Induced G1 Growth Arrest through HDAC6 Inhibition in Lung Cancer Cells.

    Science.gov (United States)

    Pai, Jih-Tung; Hsu, Chia-Yun; Hua, Kuo-Tai; Yu, Sheng-Yung; Huang, Chung-Yang; Chen, Chia-Nan; Liao, Chiung-Ho; Weng, Meng-Shih

    2015-01-01

    Disrupting lung tumor growth via histone deacetylases (HDACs) inhibition is a strategy for cancer therapy or prevention. Targeting HDAC6 may disturb the maturation of heat shock protein 90 (Hsp90) mediated cell cycle regulation. In this study, we demonstrated the effects of semisynthesized NBM-T-BBX-OS01 (TBBX) from osthole on HDAC6-mediated growth arrest in lung cancer cells. The results exhibited that the anti-proliferative activity of TBBX in numerous lung cancer cells was more potent than suberoylanilide hydroxamic acid (SAHA), a clinically approved pan-HDAC inhibitor, and the growth inhibitory effect has been mediated through G1 growth arrest. Furthermore, the protein levels of cyclin D1, CDK2 and CDK4 were reduced while cyclin E and CDK inhibitor, p21Waf1/Cip1, were up-regulated in TBBX-treated H1299 cells. The results also displayed that TBBX inhibited HDAC6 activity via down-regulation HDAC6 protein expression. TBBX induced Hsp90 hyper-acetylation and led to the disruption of cyclin D1/Hsp90 and CDK4/Hsp90 association following the degradation of cyclin D1 and CDK4 proteins through proteasome. Ectopic expression of HDAC6 rescued TBBX-induced G1 arrest in H1299 cells. Conclusively, the data suggested that TBBX induced G1 growth arrest may mediate HDAC6-caused Hsp90 hyper-acetylation and consequently increased the degradation of cyclin D1 and CDK4. PMID:25946558

  2. Single cell analysis of G1 check points-the relationship between the restriction point and phosphorylation of pRb

    International Nuclear Information System (INIS)

    Single cell analysis allows high resolution investigation of temporal relationships between transition events in G1. It has been suggested that phosphorylation of the retinoblastoma tumor suppressor protein (pRb) is the molecular mechanism behind passage through the restriction point (R). We performed a detailed single cell study of the temporal relationship between R and pRb phosphorylation in human fibroblasts using time lapse video-microscopy combined with immunocytochemistry. Four principally different criteria for pRb phosphorylation were used, namely (i) phosphorylation of residues Ser795 and Ser780 (ii) degree of pRb-association with the nuclear structure, a property that is closely related with pRb phosphorylation status, (iii) release of the transcription factor E2F-1 from pRb, and (iv) accumulation of cyclin E, which is dependent on phosphorylation of pRb. The analyses of individual cells revealed that passage through R preceded phosphorylation of pRb, which occurs in a gradually increasing proportion of cells in late G1. Our data clearly suggest that pRb phosphorylation is not the molecular mechanism behind the passage through R. The restriction point and phosphorylation of pRb thus seem to represent two separate check point in G1

  3. Robustness and backbone motif of a cancer network regulated by miR-17-92 cluster during the G1/S transition.

    Directory of Open Access Journals (Sweden)

    Lijian Yang

    Full Text Available Based on interactions among transcription factors, oncogenes, tumor suppressors and microRNAs, a Boolean model of cancer network regulated by miR-17-92 cluster is constructed, and the network is associated with the control of G1/S transition in the mammalian cell cycle. The robustness properties of this regulatory network are investigated by virtue of the Boolean network theory. It is found that, during G1/S transition in the cell cycle process, the regulatory networks are robustly constructed, and the robustness property is largely preserved with respect to small perturbations to the network. By using the unique process-based approach, the structure of this network is analyzed. It is shown that the network can be decomposed into a backbone motif which provides the main biological functions, and a remaining motif which makes the regulatory system more stable. The critical role of miR-17-92 in suppressing the G1/S cell cycle checkpoint and increasing the uncontrolled proliferation of the cancer cells by targeting a genetic network of interacting proteins is displayed with our model.

  4. Preclinical Characterization of G1T28: A Novel CDK4/6 Inhibitor for Reduction of Chemotherapy-Induced Myelosuppression.

    Science.gov (United States)

    Bisi, John E; Sorrentino, Jessica A; Roberts, Patrick J; Tavares, Francis X; Strum, Jay C

    2016-05-01

    Chemotherapy-induced myelosuppression continues to represent the major dose-limiting toxicity of cytotoxic chemotherapy, which can be manifested as neutropenia, lymphopenia, anemia, and thrombocytopenia. As such, myelosuppression is the source of many of the adverse side effects of cancer treatment including infection, sepsis, bleeding, and fatigue, thus resulting in the need for hospitalizations, hematopoietic growth factor support, and transfusions (red blood cells and/or platelets). Moreover, clinical concerns raised by myelosuppression commonly lead to chemotherapy dose reductions, therefore limiting therapeutic dose intensity, and reducing the antitumor effectiveness of the treatment. Currently, the only course of treatment for myelosuppression is growth factor support which is suboptimal. These treatments are lineage specific, do not protect the bone marrow from the chemotherapy-inducing cytotoxic effects, and the safety and toxicity of each agent is extremely specific. Here, we describe the preclinical development of G1T28, a novel potent and selective CDK4/6 inhibitor that transiently and reversibly regulates the proliferation of murine and canine bone marrow hematopoietic stem and progenitor cells and provides multilineage protection from the hematologic toxicity of chemotherapy. Furthermore, G1T28 does not decrease the efficacy of cytotoxic chemotherapy on RB1-deficient tumors. G1T28 is currently in clinical development for the reduction of chemotherapy-induced myelosuppression in first- and second-line treatment of small-cell lung cancer. Mol Cancer Ther; 15(5); 783-93. ©2016 AACR. PMID:26826116

  5. The new evidence of nucleolar ultrastructural dynamic change: fibrillar centre (FC) fusion in G1 phase and regeneration in S phase.

    Science.gov (United States)

    Wang, Fengcai; Ying, Chen; Shang, Guangbin; Jiao, Mingda; Hongfang, Zhang

    2013-06-01

    In higher eukaryotes ribosome production starts at the end of mitosis, increases during G1, is maximal in G2 (Sirri et al., 2000) and stops during prophase (Gébrane-Younès et al., 1997). But the mechanism of the change is still uncovered. Especially in the actively growing mammalian somatic cells usually contain one or several giant fibrillar centres (GFCs) with many tiny fibrillar centre (FCs) (Koberna et al., 2002; Raška et al., 2004; Casafont et al., 2007). The process how the giant fibrillar centre (GFC) and the many tiny fibrillar centres (FCs) were formed is unknown. The present results showed there were processes of FCs fusion in G1 phase and FCs regeneration in S phase respectively in the nucleoli of A 375 cells. A few FCs fused each other in late G1 phase when the process of nucleoli fusion was completed. In S phase, a lot of tiny FCs were regenerated from the periphery of GFC, separated and scattered into nucleolar matrix in late S phase and early G2 phase. The GFC was found to be coexisted with numerous tiny FCs in the nucleolus in G2 phase. The present study provided a new evidence of nucleolar dynamic change during interphase: fibrillar centre (FC) was not to be a stable state subunit of nucleolar compartment but a highly dynamic process that may be the bases of nucleolar morphological architecture organization and its function taking place. PMID:23602556

  6. Indirect semiquantitative determination of p34cdc2 levels in G1 and G2 cells of the carbohydrate-starved root meristems in Vicia faba var. minor

    Directory of Open Access Journals (Sweden)

    Justyna Polit

    2014-02-01

    Full Text Available In eukaryotes, the 34kDa kinase (p34 encoded by the cdc2 gene is a key regulator of both the onset of DNA synthesis (G1 to S phase transition and the onset of mitosis (G1 to M phase transition. Using mouse anti-human PSTAIRE and FITClabelled goat antibodies, indirect semiquantitative determination of p34cdc2 levels was performed in meristematic cells from the control (intact and excised, carbohydrate-starved main roots of Vicia faba var. minor. No evident differences in the intensity of fluorescence was found either between the G1 and G2 cells or between the control cells and the cells arrested at both Principal Control Points by carbohydrate starvation. It seems thus, that the cell cycle block induced in meristematic cells of V. faba var. minor is not correlated with the absolute level of the key cell cycle enzyme responsible for phosphory-lution of cellular proteins, but primarily with the altered activity of p34cdc2.

  7. Classic papers in combinatorics

    CERN Document Server

    Rota, Gian-Carlo

    1987-01-01

    This volume surveys the development of combinatorics since 1930 by presenting in chronological order the fundamental results of the subject proved in over five decades of original papers by:.-T. van Aardenne-Ehrenfest.-R.L. Brooks.-N.G. de Bruijn.-G.F. Clements.-H.H. Crapo.-R.P. Dilworth.-J. Edmonds.-P.Erdös.-L.R. Ford, Jr.-D.R. Fulkerson.-D. Gale.-L. Geissinger.-I.J. Good.-R.L. Graham.-A.W. Hales.-P. Hall.-P.R. Halmos.-R.I. Jewett.-I. Kaplansky.-P.W. Kasteleyn.-G. Katona.-D.J. Kleitman.-K. Leeb.-B. Lindström.-L. Lovász.-D. Lubell.-C. St. J.A. Nash-Williams.-G. Pólya.-F.P. Ramsey.-G.C. Rota.-B.L. Rothschild.-H.J. Ryser.-C. Schensted.-M.P. Schützenberger.-R.P. Stanley.-G. Szekeres.-W.T. Tutte.-H.E. Vaughan.-H. Whitney.

  8. TORSION OF THE VERMIFORM APPENDIX: A CASE REPORT

    Directory of Open Access Journals (Sweden)

    Dr. Imtiaz Wani

    2009-07-01

    Full Text Available Torsion of the vermiform appendix is a rare condition with few cases reported in the literature. Various factors predispose to torsion. Various factors predispose to torsion. We report a case of primary torsion of the vermiform appendix. The clinical presentation was indistinguishable from acute appendicitis and the diagnosis was made at operation. Appendix was preileal in position and the direction of torsion was anticlockwise. There was intrinsic torsion with no obvious factor for torsion identified. Appendectomy was performed.

  9. 40 CFR 247.10 - Paper and paper products.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Paper and paper products. 247.10... COMPREHENSIVE PROCUREMENT GUIDELINE FOR PRODUCTS CONTAINING RECOVERED MATERIALS Item Designations § 247.10 Paper and paper products. Paper and paper products, excluding building and construction paper grades....

  10. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p.

    OpenAIRE

    Cherkasova, V; Lyons, D M; Elion, E A

    1999-01-01

    In Saccharomyces cerevisiae, mating pheromones activate two MAP kinases (MAPKs), Fus3p and Kss1p, to induce G1 arrest prior to mating. Fus3p is known to promote G1 arrest by activating Far1p, which inhibits three Clnp/Cdc28p kinases. To analyze the contribution of Fus3p and Kss1p to G1 arrest that is independent of Far1p, we constructed far1 CLN strains that undergo G1 arrest from increased activation of the mating MAP kinase pathway. We find that Fus3p and Kss1p both control G1 arrest throug...

  11. CT appearance of the normal appendix in adults

    Energy Technology Data Exchange (ETDEWEB)

    Tamburrini, Stefania [Universita' di Napoli Federico II, Dip. Sc. Biomorfologiche e Funzionali, Naples (Italy); UCSD Medical Center, Department of Radiology, San Diego, CA (United States); Brunetti, Arturo [Universita' di Napoli Federico II, Dip. Sc. Biomorfologiche e Funzionali, Naples (Italy); Brown, Michele; Sirlin, Claude B.; Casola, Giovanna [UCSD Medical Center, Department of Radiology, San Diego, CA (United States)

    2005-10-01

    The aims of this study were to identify (1) the normal range of the appendix on computed tomography (CT), (2) the correlation of patient age and sex with the visibility and appearance of the appendix on CT, and (3) the normal variations in wall thickness, intraluminal content, and location of the appendix. Three hundred seventy-two outpatients underwent abdominopelvic CT. The scans were reviewed on the picture archiving and communication system and appendiceal outer-to-outer wall diameter, wall thickness, location, content and its correlation with appendix diameter were analyzed. The appendix was visualized in 305/372 patients. Its location relative to the cecum was highly variable. The diameter range was 3-10 mm; in 42% of cases the diameter was greater than 6 mm. When the intraluminal content (185/305) was visualized, the diameter was slightly superior to the mean (p=0.0156). In 329 CT scans in which oral contrast material was given, the appendix was filled by contrast material in 74/329 patients. The appendix wall thickness was measurable in 22/305 patients (average 0.15 cm). There is significant overlap between the normal and abnormal CT appearance of the appendix. Consequently the diagnosis of acute appendicitis should be based not only on the appearance of the appendix but also on the presence of secondary signs. (orig.)

  12. CT appearance of the normal appendix in adults

    International Nuclear Information System (INIS)

    The aims of this study were to identify (1) the normal range of the appendix on computed tomography (CT), (2) the correlation of patient age and sex with the visibility and appearance of the appendix on CT, and (3) the normal variations in wall thickness, intraluminal content, and location of the appendix. Three hundred seventy-two outpatients underwent abdominopelvic CT. The scans were reviewed on the picture archiving and communication system and appendiceal outer-to-outer wall diameter, wall thickness, location, content and its correlation with appendix diameter were analyzed. The appendix was visualized in 305/372 patients. Its location relative to the cecum was highly variable. The diameter range was 3-10 mm; in 42% of cases the diameter was greater than 6 mm. When the intraluminal content (185/305) was visualized, the diameter was slightly superior to the mean (p=0.0156). In 329 CT scans in which oral contrast material was given, the appendix was filled by contrast material in 74/329 patients. The appendix wall thickness was measurable in 22/305 patients (average 0.15 cm). There is significant overlap between the normal and abnormal CT appearance of the appendix. Consequently the diagnosis of acute appendicitis should be based not only on the appearance of the appendix but also on the presence of secondary signs. (orig.)

  13. Carcinoid of the Appendix During Laparoscopic Cholecystectomy: Unexpected Benefits

    Science.gov (United States)

    Haluck, Randy; Cooney, Robert N.; Minnick, Kathleen E.; Ruggiero, Francesco; Smith, J. Stanley

    1999-01-01

    Carcinoid tumors of the midgut arise from the distal duodenum, jejunum, ileum, appendix, ascending and right transverse colon. The appendix and terminal ileum are the most common location. The majority of carcinoid tumors originate from neuroendocrine cells along the gastrointestinal tract, but they are also found in the lung, ovary, and biliary tracts. We report the first case of elective laparoscopic cholecystectomy in which we found a suspicious lesion at the tip of the appendix and proceeded to perform a laparoscopic appendectomy. The lesion revealed a carcinoid tumor of the appendix. PMID:10323177

  14. Ferrofluid-Impregnated Paper Actuators

    OpenAIRE

    Ding, Zhenwen; Wei, Pinghung; Chitnis, Girish; Ziaie, Babak

    2011-01-01

    In this paper, we report on an inexpensive method of fabricating miniature magnetic actuators using ferrofluid-impregnated paper. Different types of papers (including soft tissue paper, cleanroom paper, Whatman-1 filter paper, printer paper, and newspaper) were loaded with oil-based ferrofluid, micromachined by a CO(2) laser and coated with a thin layer of parylene-C. The soaking capability of the different papers was investigated, with the soft tissue paper having the highest loading capacit...

  15. HPLC法测定150种中药材中黄曲霉毒素G2、G1、B2、B1的含量%Determination of Aflatoxin G2,G1,B2 and B1 in 150 Chinese Herbs by HPLC

    Institute of Scientific and Technical Information of China (English)

    郭巧技; 高咏莉; 王淑红

    2012-01-01

    Objective: To establish an HPLC method for the determination of aflatoxin G2 , G1 , B2 and B1 in 150 Chinese herbs. Method: After extracted by 70% methanol and purified by immunoafinity column, the aflatoxins were determined by fluorescence detection. Result: The calibration curves for aflatoxin G2 and B2 were linear within the range of 0. 15-6.00 ng · ml-1 , and those for aflatoxin G1 and B1 were linear within the range of 0. 5-20.00 ng · ml-1 . The recoveries were within the range of 85. 6% -92. 0% . Conclusion : The method is reliable, accurate and specific, and can be used in the determination of aflatoxin G2 , G1 , B2 and B1.%目的:建立了HPLC法测定150种中药材中的黄曲霉毒素G2、G1、B2、B1含量.方法:样品经70% 甲醇提取、免疫亲和色谱柱净化后,用HPLC-柱后衍生-荧光检测器测定.结果:黄曲霉毒素G2、B2在0.15~6.00 ng·ml-1范围内,黄曲霉毒素G1、B1在0.5~20.00 ng·ml-1范围内线性关系良好.回收率为85.6%~92.0%.结论:本法操作简便,结果准确、重复性好,可用于中药材中黄曲霉毒素G2、G1、B2、B1的测定.

  16. Correlation between Progesterone Dependent Cyclin G1 and PRA/PRB Expression in Endometrial Adenocarcinoma and the Related Clinical Significance%子宫内膜腺癌中孕激素依赖的cyclin G1与PRA/PRB表达的相关性及临床意义

    Institute of Scientific and Technical Information of China (English)

    方明; 于海礼; 袁东智; 徐倩; 张金虎; 何亚平; 岳利民

    2011-01-01

    Objective : To study the correlation hetween cyclin G1 and progesterone receptor isoforms PRA / PRB in endometrial adenocarcinoma in order to explore the reason for the low expression of cyclin G1 in this kind of disease. Methods : Immunohistochemistry was used to test the expression of cyclin G1 and progesterone receptor PRA / PRB in 48 cases of adenocarcinoma of endometrium including 17 cases of well-differentiated carcinoma, 19 cases of mid-differentiated carcinoma and 12 cases of poor-differentiated carcinoma. The correlation between cyclin G1 protein expression and PRA or PRB expression was analyzed. Results: In well. mid and poor differentiated adenocarcinoma of endometrium, PRA positive rates were 88. 2% ( 15/17 ), 79. 0% ( 15/19 ) and 66. 7%( 8/12 )respectively. Statistical analysis showed that there was no correlation between PRA expression and histological grade of endometrial adenocarcinoma( P>0. 05 ); PRB positive rates were 47. 1%( 8/17 ), 15. 8%( 3/19 )and 8. 3%( 1/12 ) respectively and cyclin G1 positive rates were 35. 3% ( 6/17 ), 10. 5% ( 2/19 ) and 0% ( 0/12 )respectively. The expression of both PRB and cyclin Gl were positively correlated with histological grade of endometrialadenocarcinoma( P < 0. 05 ). There was no correlation hetweencyclin G1 expression and PRA while relationship was found between cyclin G1 expression and PRB( P < 0. 05 ). Conclusion : In endometrial adenocarcinoma, the expressions of PRB and cyclin Gl protein decrease with the descending of tumor histological grade , and the expressions of both proteins are positively correlated. Thus it can be deduced that low expression of progesterone dependent cyclin G1 may he related to low expression of PRB in endometrial adenocarcinoma.%目的:探讨孕激素依赖的cyclin G1与孕激素受体亚型PRA和PRB在子宫内膜腺癌中表达的相关性及cyclin G1在子宫内膜腺癌中低表达的原因.方法:采用免疫组化SP法分别检测48例子宫内

  17. 牛初乳中IgG1、IgG2的制备色谱分离与纯化%Isolation and purification of IgG1 and IgG2 from bovine colostrums by prepare chromatography

    Institute of Scientific and Technical Information of China (English)

    王晓工; 薄金岭

    2004-01-01

    以二甲基十二烷基苄基溴化胺为预处理物质,采用DEAE Sepharose F.F离子交换色谱和Sephacryl S-200HR凝胶过滤色谱分离纯化了牛初乳中的IgG1和IgG2.用亲和高效液相色谱法测定了IgG1、IgG2的含量.

  18. The ecosystem evaluation model based on improved group-G1 and the empirical study on provinces%基于改进群组G1赋权的生态评价模型及省份实证

    Institute of Scientific and Technical Information of China (English)

    迟国泰; 闫达文

    2012-01-01

    This paper constructed the ecosystem comprehensive evaluation index system framework using driver-pressure-state-impacts-response (DPSIR) conceptual model. The comprehensive evaluation index system is formed through the selection, screening and rational analysis of ecological-system indicators. The evaluation model is established using the improved group Gl method to determine the index weight. At the same time, the paper uses empirical analysis to analyze the ecological conditions of 14 typical Chinese provinces. The contributions of this paper come from three aspects. Firstly, the paper constructed index system using DPSIR conceptual framework, and bring human demand, economic development and social progress into the system. Secondly, use the similarity degree of the ratio vectors or similarity degree of the order relation after equivalent formulation to empower the expert's knowledge and experience. Thirdly, through adding natural disaster index into the pressure layer of the index system, the paper described the effect of natural disaster on ecosystem. This improved 'he existing DPSIR model which only considered the effect of human activity on ecosystem.%利用“驱动力-压力-状态-影响-响应(DPSIR)”模型构建了生态系统综合评价指标体系框架,通过对指标海选,筛选和理性分析最终确立了生态系统的综合评价体系,应用改进群组G1方法确定指标权重,对我国14个省区的生态进行实证分析.首先,通过采用DPSIR概念框架构建指标体系,将人的需求、经济发展和社会进步等要素纳入生态系统评价中,克服现有生态评价片面关注环境与资源状况或只关注环境保护与治理状况的不足;其次,通过斌值向量相似度或等价变形后的序关系相似度给专家赋权的方式来反映专家的知识和经验;最后,通过在压力准则层中加入自然灾害指数反映自然灾害对生态的影响,改进了DPSIR模型只考虑到人类的活动

  19. Crohn's disease limited to the vermiform appendix

    DEFF Research Database (Denmark)

    Bak, Martin; Andersen, J C

    1987-01-01

    Thirteen cases of Crohn's disease confined to the vermiform appendix were seen during a 12-year period. They constituted 16.9% of patients with primary resection of the bowel for Crohn's disease in the same period, but only 0.4% of the cases of acute appendicitis. In 10 of the 13 cases...... to approach that of recurrence after resection in other parts of the intestines. Collective review of this and three other relatively large case series gave an estimated recurrence rate of 3.5%. We conclude that in Crohn's disease initially confined to the appendix the course appears to be indolent....... there was marked fibrous thickening of the appendiceal wall, and in 11 there were epithelioid cell granulomas. Appendectomy was performed in all cases. None had postoperative fistula or later manifestations of the disease within the observation time averaging 6.3 years. The recurrence rate was previously believed...

  20. FC-TRIPLEX Chagas/Leish IgG1: a multiplexed flow cytometry method for differential serological diagnosis of chagas disease and leishmaniasis.

    Directory of Open Access Journals (Sweden)

    Andréa Teixeira-Carvalho

    Full Text Available Differential serological diagnosis of Chagas disease and leishmaniasis is difficult owing to cross-reactivity resulting from the fact that the parasites that cause these pathologies share antigenic epitopes. Even with optimized serological assays that use parasite-specific recombinant antigens, inconclusive test results continue to be a problem. Therefore, new serological tests with high sensitivity and specificity are needed. In the present work, we developed and evaluated the performance of a new flow cytometric serological method, referred to as FC-TRIPLEX Chagas/Leish IgG1, for the all-in-one classification of inconclusive tests. The method uses antigens for the detection of visceral leishmaniasis, localized cutaneous leishmaniasis, and Chagas disease and is based on an inverted detuned algorithm for analysis of anti-Trypanosomatidae IgG1 reactivity. First, parasites were label with fluorescein isothiocyanate or Alexa Fluor 647 at various concentrations. Then serum samples were serially diluted, the dilutions were incubated with suspensions of mixed labeled parasites, and flow cytometric measurements were performed to determine percentages of positive fluorescent parasites. Using the new method, we obtained correct results for 76 of 80 analyzed serum samples (95% overall performance, underscoring the outstanding performance of the method. Moreover, we found that the fluorescently labeled parasite suspensions were stable during storage at room temperature, 4 °C, and -20 °C for 1 year. In addition, two different lots of parasite suspensions showed equivalent antigen recognition; that is, the two lots showed equivalent categorical segregation of anti-Trypanosomatidae IgG1 reactivity at selected serum dilutions. In conclusion, we have developed a sensitive and selective method for differential diagnosis of Chagas disease, visceral leishmaniasis, and localized cutaneous leishmaniasis.