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Sample records for aplicada ao retinoblastoma

  1. Dosimetric intercomparison between protons and electrons therapies applied to retinoblastoma; Intercomparacao dosimetrica entre terapias de protons e eletrons aplicada ao retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Braga, Flavia Vieira

    2008-07-01

    In this work we propose a construction of a simple human eye model in order to simulate the dosimetric response for a treatment with protons and electrons in a retinoblastoma cancer. The computational tool used in this simulation was the Geant4 code, in the version 4.9.1, all these package are free and permit simulate the interaction of radiation with matter. In our simulation we use a box with 4 cm side, with water, for represent the human eye. The simulation was performed considering mono energetics beams of protons and electrons with energy range between 50 and 70 MeV for protons and 2 and 10 MeV for electrons. The simulation was based on the advanced hadron therapy example of the Geant 4 code. In these example the phantom is divided in voxels with 0.2 mm side and it is generated the energy deposited in each voxel. The simulation results show the energy deliver in each voxel, with these energie we can calculate the dose deposited in that region. We can see the dose profile of, proton and electron, and we can see in both cases that for protons the position of delivered dose is well know, that happen in the position where the proton stop, for electrons the energies is delivered along the way and pass the desired position for high dose deposition. (author)

  2. Biotecnologia aplicada ao desenvolvimento de vacinas

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    Mariana de Oliveira Diniz

    2010-01-01

    Full Text Available As vacinas representam a estratégia de intervenção com a melhor relação custo-benefício até hoje aplicada em saúde pública. Avanços biotecnológicos em diversas áreas de pesquisa têm contribuído para o desenvolvimento de formulações mais seguras e eficazes. Além disso, a aplicação de ferramentas biotecnológicas no desenvolvimento de vacinas tem provocado mudanças na maneira como pensamos e produzimos esses reagentes tanto para uso em humanos como em animais. Essas tecnologias trazem perspectivas de que, em futuro próximo, vacinas para o controle de doenças infecciosas e degenerativas ainda não passíveis de prevenção possam estar disponíveis. Em particular, vacinas com efeitos terapêuticos, embora representem um enorme desafio a ser vencido, tornam-se cada vez próximas da realidade e, certamente, terão um impacto enorme no tratamento de diversas doenças, como em algumas formas de câncer.Vaccines represent the intervention strategy with the best cost-benefit ratio so far applied in public health. Biotechnological advances in various areas of vaccine research have contributed to the development of safer and more effective formulations. Moreover, application of biotechnology tools to vaccine development has caused changes in the way we think and produce these reagents both for use in humans and animals. Such technologies bring renewed perspectives that, in the near future, vaccines for the control of several non-preventable infectious and degenerative diseases will be available. In particular, the development of vaccines with therapeutic effects, although representing a huge challenge, are getting closer to reality and will have a tremendous impact in the treatment of several diseases such as some cancer forms.

  3. Diferentes Metodologias Aplicadas ao Ensino de Astronomia

    Science.gov (United States)

    Albrecht, E.; Voelzke, M. R.

    2007-08-01

    Espera-se que o educando ao final da educação básica, adquira uma compreensão atualizada das hipóteses, modelos e formas de investigação sobre a origem e evolução do Universo em que vive. O presente trabalho tem como principal objetivo compreender dentre três práticas pedagógicas adotadas no Ensino de Astronomia, na terceira série do Ensino Médio, da Escola Estadual Colônia dos Pescadores, qual melhor cumpre o papel de formação e aprendizagem para vida. A pesquisa preliminar foi através de um questionário onde o intuito foi diagnosticar o conhecimento já existente acerca do tema em questão. O questionário é composto de vinte questões dissertativas e objetivas, onde os educandos das três turmas envolvidas o responderam. Este trabalho utiliza as seguintes metodologias: a tradicional, onde o professor é um repassador de informações, fazendo uso exclusivo de lousa e giz; a segunda também de forma tradicional, porém com auxílio de multimídia para desenvolvimento das aulas e aterceira sob forma de seminários, elaborados e apresentados pelos educandos, no qual o educador faz apenas as intervenções necessárias. Ao final do trabalho os alunos responderão novamente o questionário inicial para diagnosticar dentre as três metodologias utilizadas qual apresentou melhor resultado. Os resultados preliminares obtidos, já podem ser observados e, dos 119 alunos entrevistados, as respostas obtidas são as mais diversas e evidenciam que a grande maioria nunca teve em sua vida escolar o tema Astronomia. Ao serem questionados se já haviam estudado Astronomia as respostas foram: turma A: sim 43%; turma B: sim: 21%; turma C: sim: 24%. Porém quando questionados a respeito do significado de Astronomia observou-se que: turma A: 100% de acertos; turma B: 64% acertos; turma C: 84% de acertos, demonstrando claramente a aprendizagem em diferentes esferas, não dependendo unicamente da escola. Até o presente momento, verificou-se que há interesse em

  4. Retinoblastoma

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    Aerts Isabelle

    2006-08-01

    Full Text Available Abstract Retinoblastoma is a rare eye tumor of childhood that arises in the retina. It is the most common intraocular malignancy of infancy and childhood; with an incidence of 1/15,000–20,000 live births. The two most frequent symptoms revealing retinoblastoma are leukocoria and strabismus. Iris rubeosis, hypopyon, hyphema, buphthalmia, orbital cellulites and exophthalmia may also be observed. Sixty per cent of retinoblastomas are unilateral and most of these forms are not hereditary (median age at diagnosis two years. Retinoblastoma is bilateral in 40% of cases (median age at diagnosis one year. All bilateral and multifocal unilateral forms are hereditary. Hereditary retinoblastoma constitutes a cancer predisposition syndrome: a subject constitutionally carrying an RB1 gene mutation has a greater than 90% risk of developing retinoblastoma but is also at increased risk of developing other types of cancers. Diagnosis is made by fundoscopy. Ultrasound, magnetic resonance imaging (MRI and computed tomography (CT scans may contribute to diagnosis. Management of patients with retinoblastoma must take into account the various aspects of the disease: the visual risk, the possibly hereditary nature of the disease, the life-threatening risk. Enucleation is still often necessary in unilateral disease; the decision for adjuvant treatment is taken according to the histological risk factors. Conservative treatment for at least one eye is possible in most of the bilateral cases. It includes laser alone or combined with chemotherapy, cryotherapy and brachytherapy. The indication for external beam radiotherapy should be restricted to large ocular tumors and diffuse vitreous seeding because of the risk of late effects, including secondary sarcoma. Vital prognosis, related to retinoblastoma alone, is now excellent in patients with unilateral or bilateral forms of retinoblastoma. Long term follow-up and early counseling regarding the risk of second primary

  5. Diferentes metodologias aplicadas ao ensino de astronomia no Ensino Médio

    Science.gov (United States)

    Albrecht, E.; Voelzke, M. R.

    2009-03-01

    O presente trabalho de intervenção foi realizado junto à Escola Estadual Colònia dos Pescadores na cidade de Caraguatatuba, com très turmas do terceiro ano do Ensino Médio, envolvendo 119 alunos com idades entre 16 e 19 anos. A fase inicial foi composta de um questionário de vinte questíes dissertativas e objetivas, aplicado pelo professor titular da sala, que era o mesmo nas très turmas, para diagnosticar nos educandos os conceitos prévios sobre Astronomia e, partindo destes realizar um trabalho de intervenção nas classes envolvidas utilizando, em cada uma, metodologias diferentes: (A) sob forma de seminários, elaborados e apresentados pelos educandos, no qual o educador faz apenas as intervençíes necessárias; (B) de forma tradicional, com auxílio de multimídias para desenvolvimento das aulas e a terceira (C) tradicional, fazendo uso exclusivo de lousa e giz. Ao final do trabalho os alunos responderam novamente o questionário inicial para diagnosticar dentre as très metodologias utilizadas qual apresentou melhores aplicaçíes, os resultados iniciais foram comparados com os finais. Quando questionados a respeito do significado de Astronomia observou-se inicialmente que os acertos na turma A foram de 100%, turma B: 64%, turma C: 84%, após a intervenção os acertos foram: 100%, 97% e 85% respectivamente, demonstrando que houve um avanço significativo na turma B, a turma A manteve seu índice e a turma C evoluiu, porém não tanto quanto a B. Quando interrogados sobre quantos planetas vocè acha que existem em nosso Sistema Solar? os acertos foram: turma A: 39%, turma B: 48% e turma C: 46%, após o desenvolvimento do trabalho os acertos foram 94%, 97% e 90% respectivamente. Dentro das respostas obtidas observa-se que a metodologia tradicional com o auxílio de multimeios, aplicada na turma B, demonstrou melhores resultados, sendo a mais significativa. Outra conclusão muito importante é que apesar de o tema Astronomia ser amplamente

  6. Análise do conceito de tecnologia educacional em enfermagem aplicada ao paciente

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    Aline Cruz Esmeraldo Áfio

    2014-01-01

    Full Text Available El objetivo fue analizar el concepto de tecnología educacional, producido por la Enfermería, aplicada al paciente. Se utilizó el Modelo de Evolución de Análisis del Concepto, identificándose antecedentes, atributos, y consecuentes. Se seleccionaron 13 artículos para análisis, donde se identificaron los antecedentes: deficiencia de conocimiento, escasez de tiempo de los profesionales de enfermería, optimización del trabajo da enfermería, necesidad de alcanzar metas de pacientes; atributos: herramienta, estrategia, abordaje innovador, enfoque pedagógico, mediadora de conocimientos, forma creativa de estimular la adquisición de competencias, instrumento de producción de salud; consecuentes: mejora de calidad de vida, estímulo a comportamientos saludables, empoderamiento, reflexión y vínculo. Se enfatiza la importancia de las tecnologías educacionales para la atención de enfermería, al dinamizar actividades de educación en salud.

  7. Escala multidimensional aplicada aos estudos de apreciação musical

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    Raquel Cocenas da Silva

    2009-08-01

    Full Text Available A escala multidimensional (MDS provém de uma família de técnicas de análise de proximidade de dados, obtida por meio do julgamento do participante que compara vários estímulos em vários traços, concomitantemente. A análise dos julgamentos de proximidade produz um espaço em que pontos representam a relação entre os estímulos em um espaço euclidiano. O ordenamento dos estímulos ao longo das dimensões neste espaço permite que inferências possam ser feitas sobre o universo perceptual subjacente ao grupo de pesquisa. A literatura mostra que a MDS vem ampliando o estudo da música e a mensuração de suas propriedades estruturais.

  8. UM ESTUDO DA METODOLOGIA DE ROBERT GAGNÉ APLICADA AO ENSINO DE BIOFÍSICA

    OpenAIRE

    2014-01-01

    Cada vez mais se discute a importância de que profissionais de todas as áreas desempenhem seu trabalho de forma mais efetiva com uma capacidade de resolver problemas interdisciplinarmente. A Biofísica é uma dessas áreas que envolvem conhecimentos relacionados e contextualizados de Biologia, Física, Anatomia, Fisiologia, Cinesiologia; e que proporcionam aos profissionais da área da saúde um entendimento mais completo e dinâmico sobre os processos dos sistemas do corpo humano e o funcionamento ...

  9. Escala multidimensional aplicada aos estudos de apreciação musical

    OpenAIRE

    Raquel Cocenas da Silva; José Lino Oliveira Bueno; Emmanuel Bigand; Paul Molin

    2009-01-01

    A escala multidimensional (MDS) provém de uma família de técnicas de análise de proximidade de dados, obtida por meio do julgamento do participante que compara vários estímulos em vários traços, concomitantemente. A análise dos julgamentos de proximidade produz um espaço em que pontos representam a relação entre os estímulos em um espaço euclidiano. O ordenamento dos estímulos ao longo das dimensões neste espaço permite que inferências possam ser feitas sobre o universo perceptual subjacente ...

  10. Redes Colaborativas e Cadeia de Abastecimento: Uma Análise Regional Aplicada ao Setor Vitivinicula

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    Luísa Margarida Cagica Carvalho

    2014-11-01

    Full Text Available O aumento da complexidade e da competitividade dos mercados, assim como a imprevisibilidade gerada no actual contexto económico passaram a constituir factores envolventes de acrescida exigência para as empresas. Neste cenário, a colaboração entre empresas formando redes colaborativas assumiu-se como um tema dominante na gestão da cadeia de abastecimento e tem vindo a ser reconhecida como um factor determinante para melhorar a sua força competitiva. Este artigo tem por objectivo apresentar uma visão geral da evolução da investigação relacionada com a colaboração na cadeia de abastecimento, com particular aplicação ao setor vinícola, numa perspetiva de desenvolvimento regional. Este artigo pode dar contributos que permitem servir de referencial para o desenvolvimento de metodologias e de instrumentos que permitam monitorizar o desempenho da colaboração, bem como avaliar boas práticas que resultem da sinergia da colaboração inter-empresas e que sejam passíveis de disseminação e adopção, quer em termos de políticas públicas regionais, quer em termos empresariais.

  11. DESAPROPRIAÇÃO NA PROPRIEDADE INTELECTUAL APLICADA AO DIREITO AUTORAL

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    Nivaldo dos Santos

    2013-09-01

    Full Text Available O presente artigo tem por objetivo abordar aspectos controversos que tangem à propriedade autoral. Após breve retrospecto da evolução histórica do instituto da propriedade, discute-se uma de suas maiores inovações, a Função Social da Propriedade, trazida pela nova constituição, prevista no art. 5º, XXIII: "a propriedade atenderá sua função social"; aplicando-a ao direito autoral. Aproveita-se para discutir a questão da desapropriação no que diz respeito a este tipo de propriedade intelectual, caso sua função social não seja cumprida. Tendo em vista toda a evolução de dado princípio, bem como a "mens legis" da Constituição Cidadã de 1988, fundamentada no princípio da dignidade da pessoa humana; cujos objetivos primordiais são a promoção do bem-estar de todos e o desenvolvimento nacional.

  12. ANÁLISE DAS NORMAS BRASILEIRAS DE CONTABILIDADE APLICADAS AO SETOR PÚBLICO (NBCASP: UM ESTUDO NAS UNIDADES DA FEDERAÇÃO DO BRASIL

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    Francisca Francivânia Rodrigues Ribeiro Macêdo

    2014-06-01

    Full Text Available O objetivo deste estudo é descrever a aderência das Unidades da Federação do Brasil às Normas Brasileiras de Contabilidade Aplicadas ao Setor Público. A metodologia utilizada na pesquisa configura–se como descritiva, com abordagem qualitativa, realizada por meio de pesquisa documental. Os dados da pesquisa revelam que no exercício de 2010, 2011 e 2012 o disclosure dos órgãos públicos do Estado no que concerne à aderência às Normas Brasileiras de Contabilidade Aplicadas ao Setor Público (NBCASP que convergem com as International Public Sector Accounting Standard (IPSAS ainda é incipiente. Ressalta-se que pontos importantes constantes nas normas NBCT 16.3 e NBCT 16.8, que versam, respectivamente, sobre a divulgação do PPA, LDO e Controle Interno ainda não são respeitados, mesmo que tais itens tenham sua divulgação obrigatória exigida na Constituição Federal e pela Lei de Responsabilidade Fiscal. Observou-se também que das 27 unidades federativas, apenas os Estados de Minas Gerais, Maranhão, Rio Grande do Norte, Sergipe, Bahia, Acre, Amazonas, Roraima, Tocantins, Goiás, Mato Grosso e Mato Grosso do Sul não evidenciaram a conta intitulada intangível. Nenhum dos Estados evidenciou a Demonstração do Fluxo de Caixa e a Demonstração do Resultado Econômico ou se mencionou algo sobre o sistema de custos ou a avaliação das disponibilidades e direitos e títulos de crédito. Conclui-se que os Estados brasileiros ainda não estão preparados para iniciar a divulgação obrigatória segundo as NBCASP, dado o nível de disclosure evidenciado até o momento.

  13. IVF and retinoblastoma revisited.

    NARCIS (Netherlands)

    Dommering, C.J.; Hout, A.H. van der; Meijers-Heijboer, H.; Marees, T.; Moll, A.C.

    2012-01-01

    OBJECTIVE: To evaluate the suggested association between IVF, retinoblastoma, and tumor methylation characteristics. DESIGN: Laboratory analysis. SETTING: National Retinoblastoma Center in the Netherlands. PATIENT(S): Retinoblastoma tumors from seven children conceived by IVF or intracytoplasmic spe

  14. IVF and retinoblastoma revisited

    NARCIS (Netherlands)

    Dommering, Charlotte J.; van der Hout, Annemarie H.; Meijers-Heijboer, Hanne; Marees, Tamara; Moll, Annette C.

    2012-01-01

    Objective: To evaluate the suggested association between IVF, retinoblastoma, and tumor methylation characteristics. Design: Laboratory analysis. Setting: National Retinoblastoma Center in the Netherlands. Patient(s): Retinoblastoma tumors from seven children conceived by IVF or intracytoplasmic spe

  15. Genetics Home Reference: retinoblastoma

    Science.gov (United States)

    ... of the body's cells, it is known as germinal retinoblastoma . People with this form of retinoblastoma also ... estimate that 40 percent of all retinoblastomas are germinal, which means that RB1 mutations occur in all ...

  16. Antropometria aplicada aos operadores de tratores agrícolas Anthropometrics applied to the agricultural tractors operators

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    José Fernando Schlosser

    2002-12-01

    Full Text Available O objetivo deste trabalho foi determinar o padrão antropométrico dos operadores de tratores agrícolas da Depressão Central do Rio Grande do Sul. Os operadores de tratores agrícolas foram medidos por meio de um painel constituído por duas chapas metálicas, permanecendo durante as medições em pé, eretos, com o mínimo de roupa possível e em contato com o painel. As variáveis medidas foram as seguintes: altura do corpo; altura ao nível dos olhos; altura ao nível dos olhos sentado; altura do cotovelo; alcance do braço; alcance da mão; distância pé-patela; apoio do assento. Os dados obtidos demonstram que há diferenças entre o biótipo do operador utilizado pela indústria de tratores agrícolas e o do operador da região, de forma que este último apresentou, para todas as medidas, à exceção do apoio do assento, uma média maior. Observa-se também que, para cada medida, os limites inferior e superior do intervalo onde se encontram 90% dos operadores avaliados foram, respectivamente, menores e maiores que o padrão utilizado pela indústria, caracterizando uma maior variação. Levando-se em consideração as diferenças existentes entre o perfil antropométrico dos operadores de tratores agrícolas da Depressão Central do Rio Grande do Sul e os parâmetros utilizados pela indústria, pode-se confirmar a hipótese de que os tratores agrícolas que se encontram atualmente em comercialização no Brasil podem não oferecer o conforto necessário ao operador desta região .The objective of this work was to evaluate the anthropometric measures of the agricultural tractor operators of the Central Region of Rio Grande do Sul State. The agricultural tractor operators were measured through a panel that was made of two metallic plates. During the measurement, they were standing, wearing the minimum amount clothes possible, and touching the panel. The measures body height, eyes level height (standing, eyes level height (sitting, elbow

  17. Eletromiografia aplicada aos músculos da mastigação Electromyography applied to chewing muscles

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    Juliana Malta

    2006-01-01

    Full Text Available A eletromiografia tem se mostrado um método eficaz no auxilio de diagnósticos e tratamentos de desordens musculares. Na odontologia sua principal aplicação é no tratamento das Disfunções Temporomandibulares. Os profissionais de odontologia não utilizam esta técnica por desconhecerem seus benefícios ou mesmo não terem conhecimento sobre o assunto. Um dos principais objetivos do tratamento utilizando eletromiografia é mostrar ao paciente o estado atual do músculo analisado e sua evolução durante o tratamento, até chegar a uma função satisfatória.The electromyography has if shown an effective method in the aid of diagnoses and treatments of muscular disorders. In dentistry your main application it is in Dysfunction Temporomandibular treatment. The dentistry professionals don't use this technique for they ignore their benefits or even they have not knowledge on the subject. One of the main objectives of the treatment using electromyography is to show to the patient the current state of the analyzed muscle and your evolution during the treatment, until arriving to a satisfactory function.

  18. [Retinoblastoma update].

    Science.gov (United States)

    Aerts, I; Lumbroso-Le Rouic, L; Gauthier-Villars, M; Brisse, H; Doz, F

    2016-01-01

    Retinoblastoma is the most common intraocular malignancy of infancy with an incidence of 1/15,000 births. Sixty percent of retinoblastomas are unilateral, with a median age at diagnosis of 2 years, and in most cases they are not hereditary. Retinoblastoma is bilateral in 40% of cases, with an earlier median age at diagnosis of 1 year. All bilateral and multifocal unilateral forms are hereditary and are part of a genetic cancer predisposition syndrome. All children with a bilateral or familial form, and 10-15% of children with a unilateral form, constitutionally carry an RB1 gene mutation. The two most frequent symptoms at diagnosis are leukocoria and strabismus. Diagnosis is made by fundoscopy, with ultrasound and magnetic resonance imaging (MRI) contributing both to diagnosis and assessment of the extension of the disease. Treatment of patients with retinoblastoma must take into account the various aspects of the disease (unilateral/bilateral, size, location), the risks for vision, and the possible hereditary nature of the disease. The main prognostic aspects are still early detection and adapted coverage by a multidisciplinary, highly specialized team. Enucleation is still often necessary in unilateral disease; the decision for adjuvant treatment is made according to the histological risk factors. The most important recent therapeutic advances concern conservative treatment, which is proposed for at least one of the two eyes in most bilateral cases: laser alone or in combination with chemotherapy, cryotherapy, or brachytherapy. Recently, the development of new conservative techniques of treatment, such as intra-arterial selective chemotherapy perfusion and intravitreal injections, aims at preserving visual function in these children and decreasing the number of enucleations and the need for external beam radiotherapy. The vital prognosis related to retinoblastoma is now excellent in industrialized countries, but long-term survival is still related to the

  19. Teoria da resposta ao item aplicada ao Inventário de Depressão Beck Item response theory applied to the Beck Depression Inventory

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    Stela Maris de Jezus Castro

    2010-09-01

    Full Text Available O Inventário de Depressão Beck (BDI, uma escala que mede o traço latente de intensidade de sintomas depressivos, pode ser avaliado através da Teoria da Resposta ao Item (TRI. Este estudo utilizou o modelo TRI de Resposta Gradual na avaliação da intensidade de sintomas depressivos de 4.025 indivíduos que responderam ao BDI, de modo a explorar eficientemente a informação disponível nos diferentes aspectos possibilitados pelo uso desta metodologia. O ajuste foi efetuado no software PARSCALE. Foram identificados 13 itens do BDI nos quais pelo menos uma categoria de resposta não tinha chance maior que as demais de ser escolhida, de modo que estes itens tiveram de ser recategorizados. Os itens com maior capacidade de discriminação são relativos à tristeza, pessimismo, sentimento de fracasso, insatisfação, auto-aversão, indecisão e dificuldade para trabalhar. Os itens mais graves são aqueles relacionados com perda de peso, retraimento social e idéias suicidas. O grupo dos 202 indivíduos com as maiores intensidades de sintomas depressivos foi composto por 74% de mulheres, e praticamente 84% possuíam diagnóstico de algum transtorno psiquiátrico. Os resultados evidenciam alguns dos inúmeros ganhos advindos da utilização da TRI na análise de traços latentes.The Beck Depression Inventory (BDI, a scale that measures the latent trait intensity of depression symptoms, can be assessed by the Item Response Theory (IRT. This study used the Graded-Response model (GRM to assess the intensity of depressive symptoms in 4,025 individuals who responded to the BDI, in order to efficiently use the information available on different aspects enabled by the use of this methodology. The fit of this model was done in PARSCALE software. We identified 13 items of the BDI in which at least one response category was not more likely than others to be chosen, so that these items had to be categorized again. The items with greater power of

  20. Análise de Risco aplicada aos alimentos no Brasil: perspectivas e desafios Risk Analysis applied to food safety in Brazil: prospects and challenges

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    Ana Virgínia de Almeida Figueiredo

    2011-04-01

    Full Text Available Trata-se de um estudo de caso no qual se discutiram as ideias do Comitê do Codex Alimentarius do Brasil (CCAB, coordenado pelo Instituto Nacional de Metrologia, Normalização e Qualidade Industrial (Inmetro sobre a norma Codex Alimentarius de Análise de Risco aplicada aos alimentos (AR. Os objetivos deste trabalho foram identificar e analisar a opinião dos membros do CCAB sobre AR e levantar as propostas para a adoção desta norma no país, destacando as limitações e as potencialidades locais apontadas. Os membros do CCAB mostraram-se favoráveis à iniciativa do Codex em instituir a norma AR para promover a segurança sanitária dos alimentos que transitam no mercado internacional. Houve concordância no sentido de que o governo brasileiro deva incorporar a AR como política oficial para melhorar o sistema de controle de alimentos do país e para fortalecer as exportações brasileiras de alimentos. Reconheceram que o Brasil dispõe de capacidade técnico-científica para aplicar essa norma, porém destacaram várias limitações político-institucionais. Os membros reconhecem a AR como uma iniciativa valiosa para enfrentar os riscos dos alimentos, devido a sua capacidade de aprimorar as ações de controle sanitário de alimentos realizadas pelo governo.The scope of this case study is to discuss the ideas of the Brazilian Codex Alimentarius Committee (CCAB coordinated by National Institute of Metrology, Standardization and Industrial Quality (Inmetro, with respect to the Codex Alimentarius norm on Risk Analysis (RA applied to Food Safety. The objectives of this investigation were to identify and analyze the opinion of CCAB members on RA and to register their proposals for the application of this norm in Brazil, highlighting the local limitations and potential detected. CCAB members were found to be in favor of the Codex Alimentarius initiative of instituting an RA norm to promote the health safety of foods that circulate on the

  1. Lexicologia aplicada ao ensino do léxico em português língua não materna - estudo de caso: escola do segundo ciclo da cidade do Wizi

    OpenAIRE

    2013-01-01

    Tese apresentada para cumprimento dos requisitos necessários à obtenção do grau de Doutor em Linguística – Lexicologia, Lexicografia e Terminologia O presente trabalho tem por objecto a problemática da aprendizagem e do desenvolvimento da competência lexical em PLNM. Dada a complexidade da área da Lexicologia, articulada à Lexicografia e à Terminologia, o assunto envolveria longas abordagens pelo que nos limitamos à perspectiva da Lexicologia Aplicada ao Ensino; isto é, uma Lexicologia na ...

  2. Modelagem aplicada aos processos digestivos e metabólicos do suíno Applied modeling to the digestive and metabolic processes of the pig

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    Paulo Alberto Lovatto

    2001-08-01

    Full Text Available Esse estudo bibliográfico descreve os princípios mais importantes da modelagem aplicada aos processos digestivos e metabólicos do suíno. Essa forma de modelagem se apóia, sobretudo, em dois princípios matemáticos: a linearidade e a não-linearidade, sendo representada essencialmente pelas leis de ação de massa e de Michaelis-Menten, respectivamente. Os modelos foram classificados em explicativos, temporais e paramétricos. Os explicativos descrevem a forma de integração do conhecimento, podendo ser empíricos ou mecânicos. Os temporais definem a forma de integração do conhecimento em função do tempo, podendo ser estáticos ou dinâmicos. Os paramétricos associam os parâmetros temporais e explicativos, podendo ser deterministas ou estocásticos. Os modelos de crescimento foram integrados em níveis mais subjacentes que os de digestão. Os princípios metabólicos que envolvem o anabolismo e o catabolismo tissulares, bem como as vias de utilização dos nutrientes foram melhor estudados nos tecidos proteicos. A presente revisão mostra que os modelos de digestão e de metabolismo já desenvolvidos são dinâmicos, deterministas e empíricos. Na maioria dos casos, esses modelos representam parcialmente os fenômenos digestivos e metabólicos do suíno. Os novos modelos devem integrar as formas paramétricas estocásticas, o que os aproxima mais d realidade da cinética celular nos seres vivos, considerando os nutrientes desde a ingestão até sua utilização em nível molecular.This paper reviews the main principles and characterizes the evolution of modeling applied to the swine digestive and metabolic processes. Such modeling is largely based on two mathematical principles: linearity and non-linearity, which were represented essentially by action mass and Michaelis-Menten laws. The digestive and metabolic models were classified in explanatory, temporal and parametric. Explanatory ones describe the knowledge integration, and

  3. O Sistema Nacional de Combate ao Abuso e à Exploração Sexual Infantojuvenil e o Plano Nacional: um exemplo de política pública aplicada

    Directory of Open Access Journals (Sweden)

    Thaísa Veras

    Full Text Available Este artigo tem por objetivo principal tratar de uma política pública aplicada, qual seja, o Sistema Nacional de Combate ao Abuso e à Exploração Sexual Infantojuvenil. Para tanto, inicialmente, é apresentada uma breve definição dos termos, com o intuito de esclarecer a forma como foram interpretados e utilizados no texto. Além disso, é definido o significado de políticas públicas, para que se tenha maior clareza sobre como estas podem auxiliar no entendimento do tema. Por fim, são apresentados os impactos sociais do Sistema Nacional e feito um breve relato sobre o Plano Nacional.

  4. Retinoblastoma in transgenic mice

    NARCIS (Netherlands)

    Windle, J.J.; Albert, D.M.; O'Brien, J.M.; Marcus, D.M.; Disteche, Ch.M.; Bernards, R.A.; Mellon, P.L.

    1990-01-01

    Retinoblastoma, a malignancy of the eye occurring in young children, has been widely studied as a model for genetic predisposition to cancer. This disease is caused by mutations in both alleles of an anti-oncogene (the retinoblastoma gene, Rb) that inactivate or eliminate the Rb encoded protein, pl0

  5. Treatment Options for Retinoblastoma

    Science.gov (United States)

    ... an increased risk of trilateral retinoblastoma and other cancers. A child with heritable retinoblastoma has an increased risk of ... talk with your child's doctors about the effects cancer treatment can have on your child. Regular follow-up by health professionals who are ...

  6. Treatment Option Overview (Retinoblastoma)

    Science.gov (United States)

    ... an increased risk of trilateral retinoblastoma and other cancers. A child with heritable retinoblastoma has an increased risk of ... talk with your child's doctors about the effects cancer treatment can have on your child. Regular follow-up by health professionals who are ...

  7. Stages of Retinoblastoma

    Science.gov (United States)

    ... an increased risk of trilateral retinoblastoma and other cancers. A child with heritable retinoblastoma has an increased risk of ... talk with your child's doctors about the effects cancer treatment can have on your child. Regular follow-up by health professionals who are ...

  8. General Information about Retinoblastoma

    Science.gov (United States)

    ... an increased risk of trilateral retinoblastoma and other cancers. A child with heritable retinoblastoma has an increased risk of ... talk with your child's doctors about the effects cancer treatment can have on your child. Regular follow-up by health professionals who are ...

  9. A psicanálise aplicada ao sintoma: uma resposta ética aos impasses enfrentados pelos psicanalistas na atualidade Applied psychoanalysis to the symptom: an ethical response to the challenges faced by the psychoanalysts nowadays

    Directory of Open Access Journals (Sweden)

    Alexandre Dutra Gomes da Cruz

    2011-01-01

    Full Text Available O presente artigo aborda a psicanálise aplicada em uma época de avanço do discurso capitalista, favorecedor da crescente especialização do conhecimento e promotor, no meio clínico, de uma fragmentação das grandes categorias diagnósticas. Disso resulta uma predominância de instituições cada vez mais especializadas e segregativas. Diante da massificação da terapêutica e da crescente burocratização dos procedimentos técnicos colocados em jogo, repletos de medidas avaliativas, os autores propõem a psicanálise aplicada como prática que viabiliza a inserção da psicanálise em instituições de saúde sem dissolvê-la no variado campo das psicoterapias. A formação do analista e o sintoma são considerados eixos articuladores das dimensões epistemológica, ética e política da psicanálise aplicada. Esta é considerada, a partir da orientação lacaniana, uma saída ética para os psicanalistas, confrontados com as novas demandas do mundo contemporâneo.This paper is about the applied Psychoanalysis in our time when the capitalist discourse takes place, which enhances the increasing specialization of clinical knowledge and promoter, in the clinical area, of the fragmentation of the major diagnostic categories. This carries out the development of even more specialized and segregative institutions. As far as massification of therapeutic and the increasing bureaucratization of technical procedures - full of evaluative measures - are concerned as being introduced, the authors propose the introduction of applied Psychoanalysis in institutions of health, without allowing it to be dissolved in the several fields of psychotherapies. The psychoanalyst's formation and the symptom are considered articulator axes of epistemological dimensions, ethics and politics of applied Psychoanalysis as far as lacanian orientation is concerned, which means an ethic accomplishment for the psychoanalysts when confronted to the new demands of the

  10. Uma abordagem multicritério aplicada ao problema de seleção de portfólio de projetos

    OpenAIRE

    Everton Gomede

    2014-01-01

    O Problema de Seleção de Portfólio de Projetos (PSPP) objetiva combinar os projetos disponíveis para aumentar o retorno e diminuir o risco da carteira resultante, ao mesmo tempo que respeita um conjunto de restrições. Este problema é considerado, portanto, um problema de otimização multiobjetivo. A literatura especializada discute o PSPP somente no contexto de disciplina, sendo que o método fica sob a responsabilidade da implementação. Alguns autores abordam o problema sem uma formulação mate...

  11. Drugs Approved for Retinoblastoma

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for retinoblastoma. The list includes generic names and brand names. The drug names link to NCI’s Cancer Drug Information summaries.

  12. What is Retinoblastoma?

    Science.gov (United States)

    ... cortex , allowing us to see. How does retinoblastoma develop? The eyes develop very early as babies grow in the womb. ... a certain point, these cells stop dividing and develop into mature retinal cells. Rarely, something goes wrong ...

  13. Sistemas deliberativos em perspectiva meso: a abordagem dos subsistemas aplicada aos conselhos de políticas públicas em Belo Horizonte

    Directory of Open Access Journals (Sweden)

    Eduardo Moreira da Silva

    2016-04-01

    Full Text Available Resumo Este artigo realiza um diálogo crítico com a literatura sobre sistemas deliberativos. Propomos uma abordagem em nível meso, nomeada aqui de "subsistema deliberativo", a fim de orientar as pesquisas empíricas nesse campo. Utilizamos técnicas de análise de redes sociais (ARS para aplicarmos essa abordagem ao subsistema de conselhos de políticas públicas da cidade de Belo Horizonte, Minas Gerais, Brasil. Partimos da hipótese de que os principais elos de conexão entre os conselhos seriam mecanismos informais que conectam os conselhos. Os resultados indicam que os conselheiros governamentais fazem parte de vários conselhos simultaneamente, portanto, atuam como elos de conexões no subsistema analisado. Apontamos a necessidade de realizar pesquisas futuras capazes de indicar a conexão dos conselhos com outros subsistemas.

  14. Seleção combinada univariada e multivariada aplicada ao melhoramento genético da seringueira Univariate and multivariate combined selection applied to Hevea breeding

    Directory of Open Access Journals (Sweden)

    REGINALDO BRITO DA COSTA

    2000-02-01

    Full Text Available Este trabalho objetivou estimar os coeficientes de herdabilidade e de predição genética, pelo método multivariado, e comparar os ganhos genéticos obtidos por meio da seleção entre e dentro de progênies, individual e combinada, em seringueira (Hevea brasiliensis (Willd. ex Adr. de Juss. Müell. Arg.. Vinte e duas progênies de meios-irmãos foram plantadas na Estação Experimental de Votuporanga, SP, no delineamento de blocos ao acaso, com cinco repetições e dez plantas por parcela. Aos três anos de idade foram avaliados os caracteres: número de anéis de vasos laticíferos (NA, produção de borracha seca (PB, espessura de casca (EC e circunferência do caule (CC. Os resultados demonstraram haver variabilidade significativa entre progênies quanto aos caracteres PB, EC e CC. As estimativas de coeficientes de herdabilidade e de predição genética foram mais elevadas nas progênies, com valores mais expressivos quanto aos coeficientes de predição genética. Ganhos genéticos adicionais foram obtidos pela seleção combinada. Embora os referidos ganhos tenham sido de pequena magnitude, recomenda-se o uso do método, por propiciar maior acurácia e ganho genético. Os valores mais expressivos de acurácia e ganho genético foram estimados pelo método multivariado.The objective of this paper was to estimate the heritability coefficients by the univariate method and genetic prediction by the multivariate method, as well as to compare the genetic gain obtained by individual and combined selection between and within progenies, in rubber tree (Hevea brasiliensis Willd. ex Adr. de Juss. Müell. Arg.. Twenty two half-sib progenies were established at the Experimental Station, in Votuporanga, São Paulo State, Brazil, following the randomized block design, with five replications and ten plants per plot. Three years after planting, number of latex vessel rings (NR, yield of dry rubber (YB, bark thickness (BT and stalk circunference (SC were

  15. Nanotecnologia farmacêutica aplicada ao tratamento da malária Application of pharmaceutical nanotechnology to the treatment of malaria

    Directory of Open Access Journals (Sweden)

    Lúcio Figueira Pimentel

    2007-12-01

    Full Text Available Apesar do desenvolvimento tecnológico e científico, a malária permanece como um dos maiores problemas de saúde a serem combatidos. As estratégias modernas para o controle da doença prevêem ações conjuntas, como o combate do inseto vetor, diagnóstico rápido e preciso, garantia de terapêutica adequada, redução dos casos de resistência, além do desenvolvimento de novos agentes terapêuticos e vacina e através da otimização da ação de fármacos utilizados na atualidade. Os sistemas de liberação controlada de fármacos vêm recebendo atenção especial nesta área de pesquisa, com o desenvolvimento de estratégias para a veiculação de agentes bioativos e vacinas na forma de nanodispositivos tais como lipossomas, nanopartículas e micropartículas. Diversos nanossistemas já demonstraram eficácia na otimização de vacinas e quimioterápicos destinados ao controle da malária. Este artigo de revisão tem por objetivo avaliar o estado da arte na terapêutica da malária e demonstrar o potencial da nanotecnologia farmacêutica como ferramenta destinada ao combate da doença.In spite of living in a scientific and technological era, malaria continues to be one of the worldwide greatest health challenges. The state-of-the-art policy to keep malaria under control is expected to comprise joint-strategies, such as the vector control, fast diagnosis, therapeutic guarantee, resistance cutback, drug optimization and development of new therapeutic agents and vaccines. Nano and microcarrier systems have been receiving a special attention, including the development of strategies for carrying bioactive agents, vaccines through nanodevices, such as liposomes and nanoparticles, and microdevices, such as microparticles and microemulsions. Numerous nanosystems have already substantiated their effectiveness to optimize vaccines, insecticides, and chemotherapeutic agents applied to the control of malaria. This review is intended to explain the

  16. A Análise Ergonômica do Trabalho (AET aplicada ao trabalho na agricultura: experiências e reflexões

    Directory of Open Access Journals (Sweden)

    Roberto Funes Abrahão

    Full Text Available Objetivo: este trabalho resume a experiência do Grupo de Pesquisa em Ergonomia, Trabalho e Agricultura, do Programa de Pós-Graduação da Faculdade de Engenharia Agrícola da Universidade Estadual de Campinas (UNICAMP, sobre a análise do trabalho agrícola com a utilização da Análise Ergonômica do Trabalho (AET. Método: reflexão circunstanciada com base no referencial teórico-metodológico da AET. Resultados: apresentam-se reflexões sobre a utilização da AET em um contexto de pesquisa acadêmica, focado no estudo do trabalho na agricultura, por meio de estudos sobre o trabalho na fruticultura e horticultura orgânica, na colheita manual da cana-de-açúcar, na avicultura de corte, em unidades de beneficiamento de tomates e na colheita florestal. Conclusões: a AET mostrou-se apropriada como um meio de aproximação ao objeto da pesquisa e sua utilização auxiliou na lapidação das hipóteses iniciais e no detalhamento dos procedimentos metodológicos.

  17. Protocolo de cuidados ao cliente com distúrbio respiratório: ferramenta para tomada de decisão aplicada à enfermagem

    Directory of Open Access Journals (Sweden)

    Graciele Oroski Paes

    2014-04-01

    Full Text Available Este estudo objetivou elaborar um protocolo de enfermagem voltado para a assistência a clientes em situações de distúrbio respiratório agudo e testar sua aplicabilidade. Métodos: A metodologia empregada foi quase experimental, do tipo antes-depois, que se utilizou da abordagem quantitativa, por meio da testagem do protocolo em momentos distintos. O grupo pesquisado contou com 22 enfermeiros, que atendiam aos critérios preestabelecidos para participar da pesquisa. Para o processo de validação participaram três consultores. Resultados: Os resultados apontaram para a melhora das respostas dos enfermeiros com o auxílio do protocolo, e os pareceres dos consultores avaliaram que o protocolo encontrava-se adequado considerando as variáveis avaliadas. Conclusão: Acreditamos que o protocolo para a assistência do enfermeiro a clientes com distúrbios respiratórios agudos contribui para otimização do seu cuidado, dinamizando e agilizando as intervenções adequadas para os clientes portadores do agravo.

  18. Criação e Acesso a Informação Semântica Aplicada ao Governo Eletrónico

    Directory of Open Access Journals (Sweden)

    Mário Rodrigues

    2012-01-01

    Full Text Available Os cidadãos, empresas ou serviços públicos - os clientes - que procuram informações no contexto do Governo Eletrónico visam obter respostas objetivas às suas questões. Para isso é necessário que os sistemas de pesquisa consigam manipular a informação de modo a que seja disponibilizada de uma forma eficaz e adequada às necessidades de cada cliente. Uma vez que grande parte dos documentos do governo estão escritos em formatos não estruturados e em linguagem natural, é necessário desenvolver métodos para obter e estruturar este tipo de informação. A alternativa seria indexar pelo seu texto a grande quantidade de documentos existente, uma solução desadequada no contexto do Governo Eletrónico, uma vez que assim seriam retornados frequentemente muitos resultados a cada pesquisa.Este artigo apresenta um primeiro protótipo de uma aplicação que gera informação semântica a partir de textos escritos em Português. A informação semântica gerada corresponde a um domínio de conhecimento definido por um operador humano através de uma interface gráfica, de modo a que o sistema seja adaptável às diferentes áreas de atuação do Governo Eletrónico. O conteúdo é acessível através de uma interface em linguagem natural e através de uma interface de pesquisa que aceita entradas SPARQL. Deste modo é possível aos clientes aceder diretamente ou integrar este sistema com os seus próprios sistemas de informação. A aplicação está organizada em três grandes módulos: Representação do Conhecimento que permite definir domínio de conhecimento e sua semântica e criar exemplos semente, nos textos, de conceitos do domínio de conhecimento; Processamento de Linguagem Natural que permite obter estruturas sintáticas associadas às frases em linguagem natural; e Extração e Integração Semântica que utiliza os exemplos semente para treinar classificadores estatísticos a identificar nas estruturas sintáticas os conceitos

  19. Criação e Acesso a Informação Semântica Aplicada ao Governo Eletrónico

    Directory of Open Access Journals (Sweden)

    Mário Rodrigues

    2012-01-01

    Full Text Available

    Os cidadãos, empresas ou serviços públicos - os clientes - que procuram informações no contexto do Governo Eletrónico visam obter respostas objetivas às suas questões. Para isso é necessário que os sistemas de pesquisa consigam manipular a informação de modo a que seja disponibilizada de uma forma eficaz e adequada às necessidades de cada cliente. Uma vez que grande parte dos documentos do governo estão escritos em formatos não estruturados e em linguagem natural, é necessário desenvolver métodos para obter e estruturar este tipo de informação. A alternativa seria indexar pelo seu texto a grande quantidade de documentos existente, uma solução desadequada no contexto do Governo Eletrónico, uma vez que assim seriam retornados frequentemente muitos resultados a cada pesquisa.


    Este artigo apresenta um primeiro protótipo de uma aplicação que gera informação semântica a partir de textos escritos em Português. A informação semântica gerada corresponde a um domínio de conhecimento definido por um operador humano através de uma interface gráfica, de modo a que o sistema seja adaptável às diferentes áreas de atuação do Governo Eletrónico. O conteúdo é acessível através de uma interface em linguagem natural e através de uma interface de pesquisa que aceita entradas SPARQL. Deste modo é possível aos clientes aceder diretamente ou integrar este sistema com os seus próprios sistemas de informação. A aplicação está organizada em três grandes módulos: Representação do Conhecimento que permite definir domínio de conhecimento e sua semântica e criar exemplos semente, nos textos, de conceitos do domínio de conhecimento; Processamento de Linguagem Natural que permite obter estruturas sintáticas associadas às frases em linguagem natural; e Extração e Integração Semântica que utiliza os exemplos semente para treinar classificadores estatísticos a identificar nas estruturas sint

  20. Retinoblastoma; Le retinoblastome

    Energy Technology Data Exchange (ETDEWEB)

    Desjardins, L.; Doz, F.; Schlienger, P.; Validire, P.; Quintana, E.; Zucker, J.M. [Institut Curie, 75 - Paris (France)

    1996-05-01

    Early symptoms of retinoblastoma (leukocoria, strabismus) and the various steps of the diagnosis and differential diagnosis are reviewed. Retrolental fibroplasia, larva migrans, Coats disease, and above all uveitis are the main differential diagnoses. Pathologic features that allow the diagnosis and have a bearing on the prognosis are described. Genetic factors involved in the genesis of retinoblastoma are reviewed, including recent data provides by molecular biology studies of chromosome 13. Currently available treatments include enucleation, external beam radiation, iodine-125 disks, xenon photo-coagulation, cryo-application, chemotherapy, and carbo-platinum combined with diode laser hyperthermia. The indications of each of these methods in intra- and extra-ocular retinoblastomas are discussed, as well as results and complications. Emphasis is put on the high risk of a second cancer. 47 refs., 4 figs.

  1. Genetics and management of retinoblastoma

    Directory of Open Access Journals (Sweden)

    Bakhshi Sameer

    2007-01-01

    Full Text Available Retinoblastoma is the most common intraocular tumor in childhood. In majority of early stage retinoblastoma, the eyeballs as well as vision can be preserved with chemotherapy and local intraocular therapy with laser or photocoagulation. However, more than half the patients in India and other developing nations present in advanced stage of the disease. This article reviews the genetics, clinical approach, and treatment options for retinoblastoma focussing on advances in chemotherapy for intraocular retinoblastoma (chemoreduction, as well as improvement in survival in advanced retinoblastoma with surgery, chemotherapy, radiotherapy, and bone marrow transplantation.

  2. Tabular cusum control charts of chemical variables applied to the control of surface water quality Cartas de controle cusum tabular de variáveis químicas aplicadas ao controle de qualidade de águas superficiais

    Directory of Open Access Journals (Sweden)

    Franciele A. C. Follador

    2012-10-01

    Full Text Available The aim of this study was to evaluate the application possibility of tabular CUSUM control charts in the quality control of chemical variables in surface water. It was performed bibliographic and field research to collect water samples from 2003 to 2009, totaling 30 samples, some monthly and others semi-annual in order to observe the variables that regulate water quality. It was found that these charts may be applied to control the quality of river water; showing to be effective in the perception of changes during the process, especially for small samples (n=1 which there is no repetition as in this research. It was also concluded that the Mandurim River does not presents significant levels of pollution.O objetivo do trabalho foi avaliar a possibilidade de aplicação das cartas de controle do tipo CUSUM tabular no controle de qualidade de variáveis químicas em água superficial. Foi realizada pesquisa bibliográfica e de campo com coleta de amostras de água, no período de 2003 até 2009, perfazendo 30 coletas, algumas mensais e outras semestrais, com o intuito de observar as variáveis que regulam a qualidade da água. Verificou-se que estas cartas podem ser aplicadas ao controle de qualidade de água de rio, mostrando ser eficazes na percepção de alterações durante o processo, principalmente para amostras pequenas (n=1, para as quais não há repetição, como é o caso desta pesquisa. Também se concluiu que o Rio Mandurim não apresenta poluição em níveis consideráveis.

  3. A psicologia positiva aplicada ao contexto escolar

    OpenAIRE

    Ramôa, Maria Manuel Valente Braz Sepúlveda

    2016-01-01

    Relatório de estágio apresentado à Escola Superior de Educação de Paula Frassinetti para a obtenção de grau de mestre em educação pré-escolar e ensino do 1º ciclo do ensino básico A Psicologia positiva é considerada uma nova perspetiva de investigação e intervenção, promovendo o estudo das condições e processos que contribuem para o florescimento ou funcionamento ótimo das pessoas, grupos ou instituições, pretendendo debruçar-se sobre as experiências positivas tais como as emoções positiva...

  4. SLOW-RELEASE UREA APPLIED TO SURFACE AND REGULAR UREA INCORPORATED TO SOIL ON MAIZE YIELD UREIA DE LIBERAÇÃO LENTA APLICADA SUPERFICIALMENTE E UREIA COMUM INCORPORADA AO SOLO NO RENDIMENTO DO MILHO

    Directory of Open Access Journals (Sweden)

    Eloene Rodriges Godoy

    2011-01-01

    ="JUSTIFY">A ureia aplicada sobre a superfície do solo apresenta elevadas perdas de nitrogênio (N, por volatilização de NH3. O uso de polímeros recobrindo grânulos de ureia é uma alternativa de manejo para reduzir tais perdas. O trabalho, desenvolvido em Neossolo Quartzarênico, com 6% de argila, na safra 2007/2008, no município de Jataí (GO, teve por objetivo avaliar a eficiência dos seguintes manejos: ureia incorporada ao solo e ureia revestida com polímeros, aplicada em superfície, nos aspectos agronômicos e econômicos da cultura do milho. Adotou-se o delineamento experimental em blocos casualizados, com oito repetições, totalizando 24 parcelas. A adubação nitrogenada de cobertura foi realizada em uma única aplicação, no estádio V5. A ureia incorporada ao solo proporcionou maiores valores de comprimento da espiga, diâmetro da espiga, número de grãos por espiga, massa de cem grãos, produtividade de grãos e da relação entre a receita obtida e o valor investido. O maior rendimento de grãos e a maior lucratividade foram obtidos com a aplicação da ureia incorporada ao solo. Para cada real (R$ 1,00 investido na cultura, a ureia incorporada ao solo promoveu retorno de R$ 1,37.

    PALAVRAS-CHAVE: Zea mays L.; fertilizante de liberação lenta; nitrogênio; polímeros; sistema plantio direto.

  5. [Retinoblastoma: recent advances].

    Science.gov (United States)

    Jehanne, Mathilde; Brisse, Hervé; Gauthier-Villars, Marion; Lumbroso-le Rouic, Livia; Freneaux, Paul; Aerts, Isabelle

    2014-04-01

    Retinoblastoma is the most common intraocular malignancy of infancy with an incidence of 1/15,000 to 1/20,000 births. Sixty percent of retinoblastomas are unilateral, with a median age at diagnosis of two years, and in most cases are not hereditary. Retinoblastoma is bilateral in 40% of cases, with an earlier median age at diagnosis of one year. All bilateral and multifocal unilateral forms are hereditary and are part of a genetic cancer predisposition syndrome. All children with a bilateral or familial form, and 10 to 15% of children with an unilateral form, constitutionally carry an RB1 gene mutation. The two most frequent symptoms revealing retinoblastoma are leukocoria and strabismus. Diagnosis is made by fundoscopy, with ultrasound and magnetic resonance imaging (MRI) contributing both to diagnosis and assessment of the extension of the disease. Treatment of patients with retinoblastoma must take into account the various aspects of the disease (unilateral/bilateral, size, localization…), the risk to vision and the possible hereditary nature of the disease. The main prognostic aspects are still premature detection and adapted coverage by a multi-disciplinary specialized team. Enucleation is still often necessary in unilateral disease; the decision for adjuvant treatment is taken according to the histological risk factors. The most important recent therapeutic advances concern the conservative treatment which is proposed for at least one of the two eyes in most bilateral cases: laser alone or in combination with chemotherapy, cryotherapy or brachytherapy. Recently, the development of new conservative techniques of treatment, such as intra-arterial selective chemotherapy perfusion, aims at preserving visual function in these children and decreasing the number of enucleations and the need for external beam radiotherapy. The vital prognosis related to retinoblatoma is now excellent in industrialized countries, but long-term survival is still

  6. Radiation management of retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Takemasa, Kazuhiko; Ito, Hisao; Nishiguchi, Iku; Hashimoto, Shozo; Tanaka, Yasuhiko; Oguchi, Yoshihisa (Keio Univ., Tokyo (Japan). School of Medicine)

    1992-06-01

    Forty-five patients with retinoblastoma were treated at Keio University Hospital from 1970 to 1990. Thirty-two patients had unilateral lesions and 13 had bilateral lesions. Twenty-nine patients with unilateral and 12 with bilateral lesions underwent enucleation for advanced tumor. As a result, 3 patients with unilateral retinoblastoma and all patients with bilateral disease were treated with radiotherapy (40-50 Gy) combined with or without cryotherapy and/or photocoagulation. One patient with unilateral lesion treated with radiotherapy and chemotherapy had metastases at the first visit to our clinic and was excluded from this analysis. Among 16 eyes (15 patients) treated with radiotherapy, 6 eyes had recurrence and needed retreatment. Cataract occurred in 6 of 12 eyes and good vision was preserved in 5 of 10 eyes in which function could be evaluated. (author).

  7. A música terapêutica como uma tecnologia aplicada ao cuidado e ao ensino de enfermagem La música terapéutica como una tecnología aplicada al cuidado y a la enseñanza de la enfermería Therapeutic music as a technology applied to healthcare and to the nursing teaching

    Directory of Open Access Journals (Sweden)

    Leila Brito Bergold

    2009-09-01

    Full Text Available A predominância das técnicas na assistência muitas vezes inviabiliza o estabelecimento de um cuidado expressivo que promova a humanização da prática assistencial. Este estudo abordou duas pesquisas que investigaram o uso da música como um recurso terapêutico aplicado ao cuidado e ao ensino de enfermagem: a visita musical e a dinâmica musical. Ancorou-se nas contribuições de Even Ruud sobre o contexto cultural relacionado à música e à saúde. Utilizou-se o Método Criativo-Sensível na produção de dados das duas pesquisas, realizadas respectivamente com clientes hospitalizados e enfermeiros. A visita musical constituiu-se como tecnologia para o cuidado expressivo, pois promoveu conforto, ludicidade, expressão emocional e integração entre os clientes e destes com o ambiente hospitalar. A dinâmica musical mobilizou a produção de subjetividades nos enfermeiros, que perceberam as possibilidades do uso criativo da música como um recurso tanto no âmbito do cuidado quanto no ensino de enfermagem.El predominio de la técnica en los cuidados y asistencia muchas veces dificulta el establecimiento de un cuidado expresivo que promueva la humanización de la práctica asistencial. Este estudio abordó dos pesquisas que investigaron el uso de la música como un recurso terapéutico aplicado al cuidado y a la enseñanza de la enfermería: la visita musical y la dinámica musical. Se basó en las contribuciones de Even Ruud sobre el contexto cultural relacionado a la música y a la salud. Se utilizó el Método Creativo-Sensible en la producción de datos de las dos pesquisas, realizadas respectivamente con clientes hospitalizados y enfermeros. La visita musical se constituyó como una tecnología para el cuidado expresivo, al promover bienestar, entretenimiento, expresión emocional y la integración entre los pacientes con el ambiente de hospital. La dinámica musical movilizó la producción de subjetividades en los enfermeros, quienes

  8. Retinoblastoma , our experience

    OpenAIRE

    2013-01-01

    Introduction: The retinoblastoma is the most common malignant ocular tumor of childhood with an incidence of 1: 15000 – 20000 births and it is due to a mutation on RB1 gene, a tumoral supressor gene. The average age of diagnosis is 12 months in bilateral and 24 months in unilateral cases. Late diagnosis usually means lost of eye function or even death, that’s why the early screening in every child is so critical. Goals: Review and approach the diagnosis and current treatment o...

  9. Diffuse anterior retinoblastoma: current concepts

    Directory of Open Access Journals (Sweden)

    Yang J

    2015-07-01

    Full Text Available Jing Yang,1–3 Yalong Dang,1–3 Yu Zhu,1 Chun Zhang2,3 1Department of Ophthalmology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou City, Henan Province, 2Department of Ophthalmology, Peking University Third Hospital, 3Clinical Stem Cell Research Center, Peking University Third Hospital, Beijing, People’s Republic of China Abstract: Diffuse anterior retinoblastoma is a rare variant of retinoblastoma seeding in the area of the vitreous base and anterior chamber. Patients with diffuse anterior retinoblastoma are older than those with the classical types, with the mean age being 6.1 years. The original cells of diffuse anterior retinoblastoma are supposed to be cone precursor. Patients most commonly present with pseudouveitis, pseudohypopyon, and increased intraocular pressure. The retina under fundus examination is likely to be normal, and the clinical features mimic the inflammation progress, which can often lead to misdiagnosis. The published diffuse anterior retinoblastoma cases were diagnosed after fine-needle aspiration biopsy running the potential risk of inducing metastasis. The most common treatment for diffuse anterior retinoblastoma is enucleation followed by systematic chemotherapy according to the patient’s presentation and clinical course. This review summarizes the recent advances in etiology (including tumorigenesis and cell origin, pathology, diagnosis, differential diagnosis, and new treatment. The challenges of early diagnosis and prospects are also discussed. Keywords: pathology, microenvironment, treatment, diagnosis 

  10. Molecular pathology of retinoblastoma

    Directory of Open Access Journals (Sweden)

    Kandalam Mallikarjuna

    2010-01-01

    Full Text Available Retinoblastoma (RB is an embryonic neoplasm of retinal origin. For many years, scientists have sought the fundamental origins of tumorigenesis, with the ultimate hope of discovering a cure. Indeed, these efforts have led to a significant understanding that multiple molecular and genetic aberrations, such as uncontrolled proliferation and the inhibition of apoptosis that contribute to the canonical characteristics of tumor biology. Despite these advances, a thorough understanding, such as the precise cells, which are the targets of neoplastic transformation, especially in solid tumors, is currently lacking. The focus of this review is to emphasize the molecular defects involved in the RB tumor progression and mechanisms associated with inhibition of tumor cell apoptotic processes. This review also discusses the importance of target molecules characterization and their potential therapeutic or prognostic use in RB disease.

  11. Genetic and Epigenetic Discoveries in Human Retinoblastoma.

    Science.gov (United States)

    McEvoy, Justina D; Dyer, Michael A

    2015-01-01

    Retinoblastoma is a rare pediatric cancer of the retina. Nearly all retinoblastomas are initiated through the biallelic inactivation of the retinoblastoma tumor susceptibility gene (RB1). Whole-genome sequencing has made it possible to identify secondary genetic lesions following RB1 inactivation. One of the major discoveries from retinoblastoma sequencing studies is that some retinoblastoma tumors have stable genomes. Subsequent epigenetic studies showed that changes in the epigenome contribute to the rapid progression of retinoblastoma following RB1 gene inactivation. In addition, gene amplification and elevated expression of p53 antagonists, MDM2 and MDM4, may also play an important role in retinoblastoma tumorigenesis. The knowledge gained from these recent molecular, cellular, genomic, and epigenomic analyses are now being integrated to identify new therapeutic approaches that can help save lives and vision in children with retinoblastoma, with fewer long-term side effects.

  12. A transgenic mouse model for trilateral retinoblastoma

    NARCIS (Netherlands)

    O'Brien, J.M.; Marcus, D.M.; Bernards, R.A.; Carpenter, J.L.; Windle, J.J.; Mellon, P.; Albert, D.M.

    1990-01-01

    We present a murine model of trilateral retinoblastoma. Ocular retinoblastoma and central nervous system tumors are observed in a line of mice formed by the transgenic expression of SV40 T-antigen. An oncogenic protein known to bind to the retinoblastoma gene product (p105-Rb) is specifically expres

  13. Trilateral retinoblastoma in transgenic mice

    NARCIS (Netherlands)

    Bernards, R.A.; O'Brien, J.M.; Marcus, D.M.; Niffenegger, A.S; Carpenter, J.L.; Windle, J.J.; Mellon, P.; Albert, D.M.

    1989-01-01

    Retinoblastoma, a heritable malignancy, occurs in the eyes of children at a median age of 2 years. It is a malignancy which has been widely studied as a model for genetic predisposition to cancer. The disease occurs in both heritable and nonheritable forms. Thirty to 40% of children with retinoblas

  14. Suprasellar retinoblastoma in a 5-month-old girl

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Yun Woo; Yoon, Hye-Kyung; Han, Bokyung Kim [Department of Radiology, Samsung Medical Centre, Sungkyunkwan University School of Medicine, 50 Irwon-dong, Kangnam-gu, Seoul 135-710 (Korea); Shin, Hyung-Jin [Department of Neurosurgery, Samsung Medical Centre, Sungkyunkwan University School of Medicine, Seoul (Korea)

    2002-12-01

    Trilateral retinoblastoma is the association of bilateral retinoblastomas and an intracranial primitive neuroectodermal tumour. Bilateral retinoblastomas with a pineal tumour are typical manifestations of this association. However, there may be variants, including unilateral retinoblastoma with intracranial tumour. The intracranial tumour can occur in the suprasellar or parasellar regions even before presentation of the retinal mass. We report a female infant with a suprasellar retinoblastoma and unilateral intraocular retinoblastoma. (orig.)

  15. Terapia fotodinâmica combinada com oxigenoterapia: uma abordagem no retinoblastoma?

    OpenAIRE

    Neves, Ana Rita Marques

    2016-01-01

    O retinoblastoma é o tumor intraocular maligno mais comum nas crianças e apresenta uma incidência de 1 em cada 15 000 a 20 000 nascimentos. A terapia fotodinâmica (PDT) é um tratamento promissor no que diz respeito ao retinoblastoma e apresenta-se como uma abordagem terapêutica emergente no tratamento de outras neoplasias malignas, podendo induzir morte celular através de um mecanismo que envolve o stresse oxidativo, depois da ativação luminosa das moléculas fotossensibilizadoras. A oxigenote...

  16. Lactate dehydrogenase (LDH activity in retinoblastoma

    Directory of Open Access Journals (Sweden)

    Maurya OPS

    1987-01-01

    Full Text Available LDH estimation in aqueous and serum was carried out in 10 cases of retinoblastoma and 10 control cases (normal. LDH estimation was done by calorimetric method devised by King-Wooten (1964 (1. LDH levels were quite high in serum and aqueous in retinoblastoma, as compared to control cases LDH aqueous/serum ratio was significantly higher in retinoblastoma as compared to control

  17. Análise do Ciclo de Vida (ACV aplicada ao agronegócio: uma revisão de literatura Life Cycle Assessment (LCA applied to agribusiness: a review

    Directory of Open Access Journals (Sweden)

    Edison S. Claudino

    2013-01-01

    Full Text Available A preocupação com a dimensão ambiental da sustentabilidade tem ganhado relevância em pesquisas científicas aplicadas no agronegócio. A necessidade de um framework robusto e confiável para mensuração dos impactos ambientais no agronegócio apresenta-se como tendência em países líderes na produção mundial de alimentos, como o Brasil. Neste contexto o presente artigo descreve uma revisão de literatura acerca do conceito de Análise de Ciclo de Vida - ACV, o qual está sendo amplamente utilizado e reconhecido por técnicos e pesquisadores mundiais para avaliação ambiental das cadeias de produção, permitindo várias aplicações nos sistemas produtivos. Este artigo foi elaborado a partir de uma pesquisa bibliográfica em artigos publicados em periódicos nacionais e internacionais, sítios da internet e anais de congressos cujo objetivo é descrever a importância da aplicação e difusão do framework da ACV e suas possíveis aplicações no agronegócio brasileiro. O estudo conclui que este framework ainda é pouco difundido no agronegócio interno e o número de pesquisas com esta temática é reduzido.The concern with the environmental dimension of sustainability has become more and more relevant in studies related to agribusiness. The need of a robust and trusty framework to measure the environmental impacts in the agribusiness activities is being presented as a trend in countries leaders in food production worldwide, like Brazil. In this context, this paper describes a review related to the concept of Life Cycle Assessment - LCA which is being widely recognized and used by technicians and researchers all over the world to the environmental impacts of production chains and allowing various applications on the production systems. The paper was elaborated based on a bibliographical research carried out by accessing national and international publications, websites and proceedings of scientific events. Its main goal is to

  18. GESTÃO E CONHECIMENTO NAS CIÊNCIAS SOCIAIS APLICADAS: UMA EXPERIÊNCIA DIDÁTICA RELACIONADA AO ENSINO-APRENDIZAGEM DA DISCIPLINA FILOSOFIA DA ADMINISTRAÇÃO

    OpenAIRE

    Bazanini, Roberto; Santana, Nathalia Caroline

    2015-01-01

    O artigo discute alternativas para a construção de alternativas pedagógicas para o tratamento de temas transversais no ensino-aprendizagem de filosofia nos cursos de administração. Desde 1993, a Filosofia é uma disciplina recomendada nos cursos de Administração em todo o Brasil, porém, o ensino-aprendizagem de filosofia relacionada aos negócios encontra barreiras para se firmar como disciplina relevante do currículo escolar. O problema central da pesquisa está em identificar alternativas di...

  19. Conservative treatment modalities in retinoblastoma

    Directory of Open Access Journals (Sweden)

    Bhavna Chawla

    2013-01-01

    Full Text Available Retinoblastoma is the most common primary intraocular malignancy of childhood. A potentially curable cancer, its treatment has improved significantly over the last few decades. The purpose of this article is to review the literature on various conservative treatment modalities available for the treatment of retinoblastoma and their effectiveness, when used alone or in combination. Pubmed, Medline, Embase, and the Cochrane library were searched through 2012 for published peer reviewed data on conservative treatment modalities for retinoblastoma. Various studies show that while enucleation remains the standard of care for advanced intraocular tumors, conservative modalities that can result in globe salvage and preservation of useful vision are being increasingly employed. Such modalities include systemic chemotherapy, focal consolidation with transpupillary thermotherapy, laser photocoagulation and cryotherapy, plaque brachytherapy, and delivery of local chemotherapy using subconjunctival, sub-tenon, or intra-arterial routes. When used alone or in combination, these treatment modalities can help in avoidance of external beam radiotherapy or enucleation, thus reducing the potential for long-term side effects, while salvaging useful vision. Radioactive plaque brachytherapy has an established role in selected patients with intraocular retinoblastoma. Local injections of chemotherapeutic agents via the sub-tenon or sub-conjunctival route have been used with varying degrees of success, usually as an adjunct to systemic chemotherapy. Intra-arterial ophthalmic artery delivery of melphalan has shown promising results. It is important to recognize that today, several treatment options are available that can obviate the need for enucleation, and cure the cancer with preservation of functional vision. A thorough knowledge and understanding of these conservative treatment modalities is essential for appropriate management.

  20. A clinical study of retinoblastoma

    Directory of Open Access Journals (Sweden)

    L Subha

    2015-01-01

    Full Text Available Aim: The aim was to analyze general incidence, age incidence, laterality, common mode of presentation, staging of the tumor, radiological evidence, histopathological confirmation, management and follow-up of cases, which were diagnosed as retinoblastoma. Design: Interventional case series study from April 1997 to March 2000. Materials and Methods: Detailed history regarding the symptoms such as white reflex, watering, pain, redness, protrusion of eyeball, squint, hyphema, and defective vision were obtained. Family history regarding consanguinity between parents, health of the siblings and other relatives were recorded. Ocular examination included vision, pupillary reaction, detailed fundus examination, ocular tension, and corneal diameter. Investigations included X-ray orbit and skull, computed tomography scan orbit and brain, B-scan orbit, serum and aqueous lactate dehydrogenase; enucleated eyes were subjected to holoprosencephaly (HPE. Enucleation, radiotherapy, cryotherapy and chemotherapy were modalities of treatment. The empty socket and the other apparently normal eye were examined carefully at each visit. Results: The incidence of retinoblastoma is less when compared to other diseases of the eye. There was no sex predilection. Most of the cases diagnosed were sporadic and unilateral. Age of onset is earlier for bilateral cases than unilateral cases. Consanguinity bears close relationship with bilateral involvement. Predominant clinical sign is white reflex in the pupillary area. Majority of cases presented in the second stage of the disease. Enucleation plays a greater role in the management of retinoblastoma. HPE should include several sections of the optic nerve to find out skip lesions.

  1. GESTÃO E CONHECIMENTO NAS CIÊNCIAS SOCIAIS APLICADAS: UMA EXPERIÊNCIA DIDÁTICA RELACIONADA AO ENSINO-APRENDIZAGEM DA DISCIPLINA FILOSOFIA DA ADMINISTRAÇÃO

    Directory of Open Access Journals (Sweden)

    Roberto Bazanini

    2015-06-01

    Full Text Available O artigo discute alternativas para a construção de alternativas pedagógicas para o tratamento de temas transversais no ensino-aprendizagem de filosofia nos cursos de administração. Desde 1993, a Filosofia é uma disciplina recomendada nos cursos de Administração em todo o Brasil, porém, o ensino-aprendizagem de filosofia relacionada aos negócios encontra barreiras para se firmar como disciplina relevante do currículo escolar. O problema central da pesquisa está em identificar alternativas didáticas para tratar temas atuais, buscando nos pensadores da administração conteúdos e métodos que possam ser aplicados para que a aprendizagem da disciplina se torne significativa. Por meio de pesquisa exploratória, de natureza qualitativa, análise pós-factum, o objetivo da pesquisa, inicialmente, buscou detectar as dificuldades encontradas na percepção dos alunos e professores do curso de administração pertencentes a uma universidade localizada no Grande ABC, para posteriormente, submeter os pesquisados ao ensino de filosofia com o emprego da Aprendizagem Baseada em Problemas (ABP na perspectiva da visão empreendedora. Os resultados da pesquisa, acompanhados de dados primários e secundários apontam a importância do emprego da ABP para dinamizar o processo de interatividade entre a filosofia e as demais disciplinas do curso.

  2. Atraso diagnóstico do retinoblastoma Delayed diagnosis in retinoblastoma

    Directory of Open Access Journals (Sweden)

    Karla E. S. Rodrigues

    2004-12-01

    Full Text Available OBJETIVOS: Identificar os principais sinais e sintomas do retinoblastoma e determinar o tempo médio entre o início dos sintomas e o diagnóstico. MÉTODOS: Estudo retrospectivo, com revisão dos prontuários das crianças diagnosticadas com retinoblastoma entre janeiro de 1991 e junho de 2000 no Departamento de Pediatria do Hospital do Câncer de São Paulo. Análise estatística: teste t de Student, análise e variância, teste de Tukey-HSD (honest significant differences, teste de Levene, análise de regressão linear, curva ROC, regressão logística e análise de sobrevida pelo método de Kaplan-Meier. RESULTADOS: Foram revisados 327 prontuários, sendo 171 pacientes do sexo masculino. A idade média foi de 25 meses. Doença localizada foi verificada em 269 pacientes. Os sintomas mais freqüentes foram leucocoria (79%, estrabismo (10,7% e tumoração (3,4%. O tempo médio de queixa foi de 5,8 meses. Pacientes maiores de 2 anos de idade apresentaram maior tempo de queixa em relação aos lactentes (7,2 meses versus 4,7 meses; p = 0,001. Pacientes com estrabismo tiveram maior tempo de queixa (8,8 meses em comparação com pacientes com tumoração (2,3 meses ou leucocoria (5,6 meses (p = 0,014. Pacientes com doença metastática apresentaram maior tempo de queixa (10,6 meses; p OBJECTIVES: To identify the main symptoms of retinoblastoma and to determine the mean time between symptom onset and diagnosis (lag time. PATIENTS AND METHODS: We carried out a retrospective analysis of the patients diagnosed with retinoblastoma between January 1991 and June 2000, at the Pediatric Department of the Hospital do Câncer, São Paulo, Brazil. Statistical analyses performed were: Student's t test, ANOVA, Tukey-HSD test (honest significant differences, Levene's test, multiple regression, ROC curve, logistic regression, Kaplan-Meier, and log rank. RESULTS: 327 medical records (171 males were reviewed. The mean age was 25 months. Localized disease was

  3. Desenvolvimento de uma arquitetura embarcada reativa para agentes autônomos inteligentes, aplicada ao futebol de robôs - DOI: 10.4025/actascitechnol.v31i2.371

    Directory of Open Access Journals (Sweden)

    Claudio Jose Biazus

    2009-06-01

    Full Text Available Dentre as principais dificuldades encontradas para a construção de sistemas multiagentes em que existe a disponibilidade de um sistema de visão local, como é o caso de algumas categorias de futebol de robôs, destacam-se: necessidade de resposta em tempo real para identificação dos objetos em cena, conhecimento do ambiente, distribuição das competências de controle entre os comportamentos reativos a cargo de cada agente. O presente artigo descreve a implementação de uma arquitetura embarcada reativa para controle de Sistemas Multiagentes equipados com sistema de visão local e dotados de sensores, e sua aplicação em ambientes de futebol de robôs. Neste trabalho, foram descritas as técnicas de processamento digital de imagens e a arquitetura proposta para satisfazer às restrições relacionadas ao problema.

  4. ELETROCOAGULATION-FLOTATION APPLIED TO THE TREATMENT OF WASTEWATER GERATED IN PLASTIC RECYCLING INDUSTRY = ELETROCOAGULAÇÃO-FLOTAÇÃO APLICADA AO TRATAMENTO DE EFLUENTES GERADOS NA INDÚSTRIA DE RECICLAGEM DE PLÁSTICOS

    Directory of Open Access Journals (Sweden)

    Francisco Javier Cuba Teran

    2009-01-01

    ínio seguida da formação de flocos de impurezas, os quais foram separados da fase líquida por flotação devido à formação de microbolhas de hidrogênio, geradas na eletrólise da água. A eficiência do tratamento foi verificada em termos de remoção de Demanda Química de Oxigênio-DQO, nitrogênio amoniacal e turbidez alcançando valores de 80%, 80% e 90% respectivamente. Também houve desinfecção, sendo esta uma característica favorável adicional do processo. Os fatores responsáveis pelo desempenho estão relacionados ao tempo de detenção do efluente sob passagem de corrente, à distância entre os eletrodos, ao pH e a intensidade da corrente elétrica.

  5. Desenvolvimento de uma arquitetura embarcada reativa para agentes autônomos inteligentes, aplicada ao futebol de robôs = Development of an onboard reactive architecture for intelligent autonomous agents, applied to robot soccer

    Directory of Open Access Journals (Sweden)

    Claudio Jose Biazus

    2009-01-01

    Full Text Available Dentre as principais dificuldades encontradas para a construção de sistemas multiagentes em que existe a disponibilidade de um sistema de visão local, como é o caso de algumas categorias de futebol de robôs, destacam-se: necessidade de resposta em tempo realpara identificação dos objetos em cena, conhecimento do ambiente, distribuição das competências de controle entre os comportamentos reativos a cargo de cada agente. O presente artigo descreve a implementação de uma arquitetura embarcada reativa paracontrole de Sistemas Multiagentes equipados com sistema de visão local e dotados de sensores, e sua aplicação em ambientes de futebol de robôs. Neste trabalho, foram descritas as técnicas de processamento digital de imagens e a arquitetura proposta para satisfazer às restrições relacionadas ao problema.There are a lot of difficulties that must be faced during the development of multi-agent systems equipped with local vision, as is the case of some robotic soccer leagues, such as: the real-time constraints for recognition of scene objects, acquisition of environment knowledge, and the distribution and allocation ofcontrol competencies among reactive behaviors under the responsibility of each agent. This article describes the implementation of an onboard reactive control architecture for a multiagent system, equipped with local vision camera and local sensors. This multiple agent system is applied in robot soccer environments. The work describes the digital image processing techniques as well as the proposed control architecture that satisfy the constraints related with this kind of application.

  6. Probabilidade e Estatística Aplicada à Hidrologia

    OpenAIRE

    Guimarães, Rita Cabral

    2011-01-01

    Esta pubilicação pretende ser um manual de auxilio aos alunos na disciplinas de Hidrologia, Hidrologia Agrícola e Interpretação da Paisagem na área da probabilidade e estatistica aplicada à Hidrologia. Abordam-se temas básicos da teoria da estatística e da probabilidade para depois estes serema aplicados na resolução de problemas ligados à Hidrologia

  7. Bordieu e a lingüística aplicada

    OpenAIRE

    Maria de Lourdes Otero Brabo Cruz

    1991-01-01

    Procura-se no presente trabalho: (a) analisar as concepções de Bourdieu no que diz respeito à competência lingüística e ao tratamento dos fatos lingüísticos; (b) traçar um paralelo entre essas concepções e a lingüística aplicada; e (c) definir a sua postura como sendo a de um lingüista aplicado.

  8. Bordieu e a lingüística aplicada

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Otero Brabo Cruz

    2001-02-01

    Full Text Available

    Procura-se no presente trabalho: (a analisar as concepções de Bourdieu no que diz respeito à competência lingüística e ao tratamento dos fatos lingüísticos; (b traçar um paralelo entre essas concepções e a lingüística aplicada; e (c definir a sua postura como sendo a de um lingüista aplicado.

  9. Nested-PCR do gene que codifica o antígeno b aplicada ao diagnóstico da tuberculose pulmonar Nested-PCR for gene that encodes the antigen b applied to the diagnosis of pulmonary tuberculosis

    Directory of Open Access Journals (Sweden)

    Karla Valéria Batista Lima

    2007-04-01

    Full Text Available A reação em cadeia da polimerase usada para amplificação de uma seqüência interna de um fragmento previamente amplificado (nested-PCR foi investigada como uma alternativa complementar a pesquisa de bacilos álcool ácido resistentes e a cultura do Mycobacterium tuberculosis em meio de Lowenstein-Jensen. Foram investigadas 144 amostras de escarro de pacientes suspeitos de tuberculose encaminhados ao Laboratório de Tuberculose do Instituto Evandro Chagas em Belém, no período de junho de 2002 a dezembro de 2003. Das 144 amostras, 121 foram caracterizadas como tuberculose, 119 foram positivas na cultura, 95 na baciloscopia e 128 na nested-PCR. A sensibilidade da nested-PCR foi 96% (116/121, enquanto a especificidade foi 48% (11/23. A nested-PCR poderá ser uma ferramenta complementar para o diagnóstico da tuberculose, pois apresenta sensibilidade equivalente à cultura, no entanto, necessita de maiores avaliações visando minimizar o número de resultados falso-positivos.The polymerase chain reaction used for amplifying an internal sequence of a previously amplified fragment (nested-PCR was investigated as a complementary alternative for searching for alcohol-acid resistant bacilli and Mycobacterium tuberculosis cultures in Lowenstein-Jensen medium. 144 sputum samples were investigated from patients with suspected tuberculosis that were sent to the Tuberculosis Laboratory of the Evandro Chagas Institute in Belém, between June 2002 and December 2003. From the 144 samples, 121 were characterized as tuberculosis: 119 were positive in cultures, 95 under bacilloscopy and 128 using nested-PCR. The sensibility of the nested-PCR was 96% (116/121, while the specificity was 48% (11/23. Nested-PCR may be a complementary tool for diagnosing tuberculosis, since it presents sensitivity equivalent to that of cultures. However, further evaluations are needed with the aim of minimizing the number of false-positive results.

  10. Análise de imagem aplicada ao estudo do comportamento de leitões em abrigo escamoteador Analysis of image applied to the study of the behaviour of piglets in the creep

    Directory of Open Access Journals (Sweden)

    Héliton Pandorfi

    2004-08-01

    Full Text Available O objetivo geral desta pesquisa foi avaliar o comportamento de leitões em diferentes sistemas de aquecimento por meio da análise de imagem. A pesquisa foi desenvolvida na sala da maternidade, com 80 animais entre o nascimento e o desmame. Os tratamentos adotados para a avaliação da eficiência dos diferentes sistemas de aquecimento do abrigo escamoteador foram: piso térmico, lâmpada incandescente, resistência elétrica e lâmpada de infravermelho. Os dados ambientais foram registrados no ambiente externo, na sala da maternidade e no interior de cada abrigo escamoteador avaliado, ao longo de 19 dias. O delineamento experimental foi em blocos casualizados, com quatro tratamentos e 18 blocos, e as médias foram comparadas pelo teste de Tukey (P The objective of this research was to evaluate the behavior of piglets in different heating systems using the image analysis and geostatistics. This research was developed in a farrowing house, with 80 piglets between birth and weaning. The treatments were different heating systems: heat mat, standard heat lamp, electric resistance and infrared lamp. The climatic data were taken inside and outside the farrowing house and inside each studied creep, during two periods of 19 days long. The statistical design of the experiment was a randomized block with four treatments and 18 blocks. A Tukey test with 1% of probability was performed to compare the results averages. The average values of dry bulb temperature showed that the environment with heat mat presented a lesser temperature in the generated microclimate, followed by the standard heat lamp, electric resistance and infrared lamp. The three first treatments were recommended for better comforting for the piglets in the farrowing house. The behavior of the piglets was evaluated using the image analysis, and indicated that the best treatment was the heat mat as in the exchanges of sensible heat for conduction (contact, promoting better condition of

  11. Influência do capital intelectual na avaliação de desempenho aplicada ao setor hospitalar The influence of intellectual capital in performance evaluation: a case-study in the hospital sector

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Grespan Bonacim

    2010-06-01

    Full Text Available Este estudo contribui com a adaptação, para as entidades públicas, de uma ferramenta de avaliação de desempenho sedimentada nas empresas privadas. O objetivo é demonstrar como pode ser mensurado o impacto da atividade de ensino no valor econômico agregado por um hospital universitário público à sociedade. Para tanto, o texto foi dividido em quatro partes, além dos aspectos introdutórios, metodológicos e das considerações finais. Inicialmente, será contextualizado o setor hospitalar, mais especificamente os hospitais universitários públicos. Logo após, serão apresentadas as definições, a natureza e mensuração do capital intelectual, seguidas da demonstração dos principais modelos avaliação de desempenho econômico. Finalmente, será apresentado um modelo adaptado, sob o enfoque da gestão baseada no valor, propondo ajuste nos indicadores de retorno e do respectivo investimento, demonstrando os impactos da gestão do capital intelectual e da atividade de ensino no resultado econômico de tais instituições. O estudo foi elaborado por intermédio de uma metodologia consubstanciada em uma pesquisa bibliográfica, sob o prisma do método de procedimento comparativo descritivo. Ressalta-se, por fim, a importância da prestação de contas para a sociedade quanto ao uso do recurso público.This paper contributes to public institutions with the adaptation of a performance evaluation tool based on private companies. The objective is to demonstrate how the impact of an educational activity might be measured in the economic value added for the society of a public university hospital. The paper was divided in four parts, despite the introductory and methodological aspects and the final remarks. First, the hospital sector is explained, specifically in the context of the public university hospitals. Then, the definitions, the nature and measure of the intellectual capital are presented, followed by the disclosure of the main

  12. Prática baseada em evidências, aplicada ao raciocínio diagnóstico Práctica basada en evidencia aplicada en el raciocinio diagnóstico Evidence-based practice applied to diagnostic reasoning

    Directory of Open Access Journals (Sweden)

    Diná de Almeida Lopes Monteiro da Cruz

    2005-06-01

    Full Text Available As enfermeiras aplicam conhecimento, habilidades perceptuais e cognitivas para analisar dados dos pacientes e propor intervenções de enfermagem. Os resultados dessas intervenções dependem da adequação de sua proposição, o que, por sua vez, depende da acurácia das interpretações dos dados dos pacientes. O objetivo deste artigo é discutir a aplicação de princípios da prática baseada em evidência (PBE às decisões diagnósticas no cuidado de enfermagem, utilizando estudo de caso escrito. Os princípios da prática baseada em evidências, na definição do diagnóstico, são: a validade de um teste diagnóstico; a capacidade de o teste discriminar aqueles que apresentam uma resposta específica; a capacidade de o teste estimar a magnitude da resposta; e a adequação do teste diagnóstico ao contexto clínico. Lacunas no conhecimento de enfermagem limitam a aplicação dos princípios da PBE na avaliação clínica e no diagnóstico de enfermagem. As autoras apresentam uma estrutura para exemplificar o uso da PBE no raciocínio diagnóstico.Las enfermeras aplican conocimientos, habilidades preceptivas y cognoscitivas para analizar los datos de los pacientes y proponer intervenciones de enfermería. Los resultados de esas intervenciones dependen del refinamiento de las preposiciones que, a su vez, depende del discernimiento de las interpretaciones de los datos de los pacientes. El propósito de este artículo es aplicar el modelo de la práctica basada en la evidencia (PBE en el raciocinio diagnóstico, utilizando estudio de caso escrito. Los principios de la PBE en la definición del diagnóstico son: validad del test diagnóstico; la capacidad del test en estimar la magnitud de la respuesta; y la adecuación del test diagnóstico al contexto clínico. Lagunas en el conocimiento de enfermería limitan la aplicación de los principios de la PBE en la evaluación clínica y en el diagnóstico de enfermería. Las autoras presentan una

  13. Recent Trends in the Management of Retinoblastoma

    Directory of Open Access Journals (Sweden)

    Samuray Tuncer

    2015-04-01

    Full Text Available Retinoblastoma is the most common intraocular malignancy of childhood. Significant progress has been made in the past few years in the management of retinoblastoma. Thus, the patients’ survival and ocular salvage rates have shown marked improvement. The current treatment strategies for retinoblastoma include enucleation, intravenous chemoreduction, and intra-arterial chemotherapy. Enucleation is generally reserved for eyes with extensive retinoblastoma, in which there is no hope for useful vision. From mid-1990s, intravenous chemoreduction has been used efficiently in the management of group A, B, or C, and some D eyes according to the International Classification of Retinoblastoma. Intravenous chemoreduction combined with focal treatments (cryotherapy, transpupillary thermotherapy, and brachytherapy have provided favorable tumor control for most of these eyes. In 2008, superselective intra-arterial chemotherapy has been popularized. This treatment involves injection of a chemotherapeutic agent (mostly melphalan after selective catheterization of the ophthalmic artery under neuroradiologic intervention. This provides higher success in advanced group D and Group E eyes that have relatively lower rate of tumor control with conventional intravenous chemoreduction. In conclusion, significant advances that have emerged recently in the management of retinoblastoma led to an increase in ocular salvage rates. Recently, popularized modality of intra-arterial chemotherapy offers hope in the control of eyes with advanced retinoblastoma. (Turk J Ophthalmol 2015; 45: 71-6

  14. Late onset retinoblastoma presenting with vitreous haemorrhage

    DEFF Research Database (Denmark)

    Bagger, Mette; Prause, Jan Ulrik; Heegard, Steffen;

    2012-01-01

    in the retina. A vascularized gelatinous mass was revealed after vitrectomy. Later the patient developed white cysts in the anterior chamber and histological findings were indicative of a retinoblastoma. The patient was enucleated and the diagnosis of retinoblastoma was confirmed. Intraocular surgery in young...... people with unknown retinoblastoma enhances the risk of metastasis development, orbital recurrence and death. Unexplained vitreous haemorrhage can obscure the view of a tumour but ultrasonic findings of a retinal mass calls for further imaging e.g. through MRI. The case illustrates the importance...

  15. Evolução da terapêutica do retinoblastoma Development in the treatment of retinoblastoma

    Directory of Open Access Journals (Sweden)

    Célia Beatriz Gianotti Antoneli

    2003-08-01

    Full Text Available OBJETIVO: Mostrar a evolução do tratamento do retinoblastoma ao longo de uma década e suas implicações na sobrevida global e na preservação da visão. MÉTODOS: Trezentos e dezenove pacientes foram avaliados no estudo. Analisamos 257 pacientes portadores de retinoblastoma admitidos no Hospital do Câncer, entre janeiro de 1986 a dezembro de 1996, divididos em dois grupos de acordo com tratamento a que foram submetidos (1986 a 1990 e 1991 a 1996. Analisamos ainda 62 pacientes portadores de retinoblastoma intra-ocular entre janeiro de 1996 e maio de 2000 que foram submetidos a quimiorredução, aliada a medidas oftalmológicas, sem enucleação ou radioterapia externa. RESULTADOS: Em pacientes com doença intra-ocular, nos dois períodos, não houve significância estatística com relação à sobrevida. Analisamos 59 pacientes com retinoblastoma extra-ocular, de acordo com a classificação do CCG (Children´s Cancer Group, e a sobrevida global em 5 anos foi maior no 2º período, (63,7% vs. 41%; p= 0,059. Entre 1996 e 2000 analisamos 62 pacientes com retinoblastoma intra-ocular com visão preservada, sendo 47 (75,8% com doença bilateral e 15 (24,2% com tumor unilateral. Os portadores de tumor bilateral tiveram uma taxa de preservação de visão de 49,8%, versus 26,7% para os pacientes com tumor unilateral. CONCLUSÕES: Tumores intra-oculares mantêm altas taxas de cura e preservação da visão. Novas combinações de drogas poderão melhorar a sobrevida dos extra-oculares. Quanto às perspectivas futuras, há pesquisas envolvendo terapia gênica/molecular e terapêutica anti-angiogênese. Apesar de toda a evolução, o diagnóstico precoce é a arma mais poderosa, na cura dos portadores deste tumor.PURPOSE: The management of retinoblastoma (Rb has gradually changed over the past years achieving a satisfactory result (increasing overall survival and avoiding enucleations. METHODS: Three hundred and nineteen patients with retinoblastoma

  16. Heritable retinoblastoma and accelerated aortic valve disease

    Science.gov (United States)

    Abeyratne, L R; Kingston, J E; Onadim, Z; Dubrey, S W

    2013-01-01

    Heritable retinoblastoma is associated with a germline mutation in the tumour suppressor gene RBI. The Rb protein (pRb) arises from the RB1 gene, which was the first demonstrated cancer susceptibility gene in humans. 1 Second primary malignancies are recognised complications of retinoblastoma. Furthermore, pRb is implicated in valve remodelling in calcific aortic valve disease. 2 3 We report a family with hereditary retinoblastoma and associated secondary primary malignancies. There are two interesting aspects to this family. The first is the concept of ‘cancer susceptibility genes’; the RBI gene being the first reported in humans. A further feature of note is that two family members also have bicuspid aortic valves. We discuss a potential association between the gene defect responsible for retinoblastoma (with its associated propensity for further malignancies) and accelerated deterioration of the bicuspid aortic valve in the proband carrying this gene defect. PMID:23595191

  17. Reflexões Sobre a Pesquisa Qualitativa Aplicada ao Turismo

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    Maria Lucia Bastos Alves

    2011-12-01

    Full Text Available ResumoConsiderando que os métodos qualitativos têm por princípio o processo indutivo baseado na descoberta e na compreensão das ações humanas em suas diferentes perspectivas culturais, o artigo tem como objetivo contribuir com reflexões de ordem metodológica no campo da pesquisa qualitativa com o intuito de mostrar como diferentes procedimentos metodológicos, com suas respectivas técnicas de investigação, possibilitam maiores aprofundamentos e novas sensibilidades para os estudos do turismo.

    Palavras-chave: métodos qualitativos; técnicas; turismo.

     AbstractWhereas qualitative methods are in principle the inductive process based on the discovery and understanding of human actions in their different cultural perspectives, the article aims to contribute to reflections on methodological issues in the field of qualitative research in order to show how different procedures methodologies, with their investigative techniques, and new insights allow greater sensitivity for studies of tourism.Keywords: qualitative methods; techniques; tourism. ResumenMientras que los métodos cualitativos son, en principio, el proceso inductivo basado en el descubrimiento y la comprensión de las acciones humanas en sus diferentes perspectivas culturales, el artículo tiene como objetivo contribuir a la reflexión sobre cuestiones metodológicas en el campo de la investigación cualitativa con el fin de demostrar cómo diferentes procedimientos metodologías, con sus técnicas de investigación y nuevos conocimientos permiten una mayor sensibilidad para los estudios de turismo.

    Palabras clave: métodos cualitativos; las técnicas; el turismo.

  18. Entropia aplicada ao reconhecimento de padrões em imagens

    OpenAIRE

    Lucas Assirati

    2014-01-01

    Este trabalho faz um estudo do uso da entropia como ferramenta para o reconhecimento de padrões em imagens. A entropia é um conceito utilizado em termodinâmica para medir o grau de organização de um meio. Entretanto, este conceito pode ser ampliado para outras áreas do conhecimento. A adoção do conceito em Teoria da Informação e, por consequência, em reconhecimento de padrões foi introduzida por Shannon no trabalho intitulado \\"A Mathematical Theory of Communication\\", publicado no ano de 194...

  19. Biotecnologia aplicada ao melhoramento genético do cafeeiro

    OpenAIRE

    2011-01-01

    O melhoramento genético do cafeeiro mediante técnicas convencionais é trabalhoso e demorado. A biotecnologia oferece estratégias alternativas para auxiliar na multiplicação e no desenvolvimento de novas variedades com resistência a estresses bióticos e abióticos, melhor qualidade de bebida e maturação mais uniforme dos frutos. As técnicas de cultura de tecidos têm possibilitado a obtenção de grande número de plantas e a garantia da uniformidade genética do material. O emprego de marcadores mo...

  20. Ondas localizadas aplicadas aos meios difrativos/dispersivos

    OpenAIRE

    2004-01-01

    Resumo: Neste trabalho de tese, realizamos um estudo teorico sistematico das chamadas Ondas Localizadas (ou Ondas Não Difrativas), bem como suas possiveis aplicações em optica, abrangendo feixes e pulsos. Os resultados são enumerados a seguir: (I) Um metodo matematico simples foi desenvolvido, unificando os diferentes tipos de ondas - subluminais, luminais e superluminais - possibilitando a obtenção de novas soluções localizadas para a equação de onda, em particular para as equações de Max...

  1. Retinoblastoma

    Science.gov (United States)

    ... Uveitis Focus On Pediatric Ophthalmology Education Center Oculofacial Plastic Surgery Center Laser Surgery Education Center Redmond Ethics Center ... Uveitis Focus On Pediatric Ophthalmology Education Center Oculofacial Plastic Surgery Center Laser Surgery Education Center Redmond Ethics Center ...

  2. Retinoblastoma: genetic considerations and report of a new animal model

    NARCIS (Netherlands)

    Albert, D.M.; O'Brien, J.M.; Marcus, D.M.; Bernards, R.A.

    1990-01-01

    Retinoblastoma is the most common primary, intraocular neoplasm of childhood. Histologically, retinoblastoma resembles, in many respects, other pediatric malignancies such as medulloblastoma and neuroblastoma. These tumors are composed of small, basophilic cells with scanty cytoplasm and often form

  3. Preliminary Report of Molecular Detection of Retinoblastoma Gene Mutations

    Institute of Scientific and Technical Information of China (English)

    1994-01-01

    To develop gene diagnosis for retinoblastoma predisposition, it is necessary to disclose the retinoblastoma gene mutations or deletions in detail. Genomic DNA from tumor and peripheral white blood cells in 33 patients with retinoblastoma was detected with 3.8kb probe derived from 3' end of retinoblastoma gene cDNA. The gene abnormalities, including deletion, partial deletion and rearrangement, were found in 18 patients. Further research will be aimed at microdeletions or mutations for those patients wti...

  4. Retinoblastoma Registry report--Hospital Kuala Lumpur experience.

    Science.gov (United States)

    Jamalia, R; Sunder, R; Alagaratnam, J; Goh, P P

    2010-06-01

    Retinoblastoma is a childhood ocular cancer. The aim of this paper is to describe the clinical and epidemiological characteristics of patients with retinoblastoma in a major paediatric ophthalmology center in the country. Retrospective information was collected through the retinoblastoma registry. Late presentation with advanced staging is a major problem.

  5. Achados histopatológicos em retinoblastoma Histopathologic findings in retinoblastoma

    Directory of Open Access Journals (Sweden)

    João Pessoa de Souza Filho

    2005-06-01

    Full Text Available OBJETIVO: Descrever e analisar os principais achados histopatológicos no retinoblastoma com ênfase na invasão de nervo óptico e túnicas oculares, correlacionando-os ao tratamento realizado. MÉTODOS: Vinte e oito olhos com diagnóstico de retinoblastoma tratados por enucleação na Universidade Federal de São Paulo entre dezembro de 2000 e outubro de 2002 foram submetidos à revisão histopatológica. Os dados clínicos incluiram idade, sexo, raça, lateralidade e tratamento realizado. Na revisão histopatológica foram avaliados neovascularização da íris e seio camerular, grau de diferenciação do tumor e invasão do nervo óptico e coróide de acordo com a classificação de Khelfaoui. RESULTADOS: De 27 pacientes, 13 (48,1% eram do sexo masculino e 14 (51,9% eram do sexo feminino, 16 (59,3% eram da raça branca, 5 (18,5% eram da raça amarela, 4 (14,8% eram pardos e 2 (7,4% eram negros. A média da idade dos pacientes foi de 22,7 meses, sendo 13 casos bilaterais e 14 casos unilaterais. Com relação ao tratamento utilizado, 13 (46,4% olhos foram submetidos a enucleação primária e 15 (53,6% tiveram tratamento prévio. Na avaliação histopatológica, 15 (53,6% tumores eram bem diferenciados. Neovascularização da íris e/ou seio camerular foram observados em 60,6% e necrose e calcificação em 89,2% dos casos. O envolvimento da coróide foi observado em 18 (64,2% dos casos (grau II e III e a invasão de nervo óptico em 8 (28,5% (graus II, III e IV. Dos casos submetidos ao tratamento prévio 40% tinham algum grau de invasão das túnicas oculares e apenas 6,67% apresentavam simultaneamente invasão do nervo óptico e das túnicas oculares. Entretanto nos olhos que foram submetidos à enucleação primária 30,7% apresentavam invasão das túnicas oculares e 53,8% apresentavam simultaneamente invasão do nervo óptico e das túnicas oculares. CONCLUSÃO: Neovascularização, necrose e calcificação foram achados mais comumente

  6. Anti-tumor activity of arginine deiminase via arginine deprivation in retinoblastoma.

    Science.gov (United States)

    Kim, Jeong Hun; Kim, Jin Hyoung; Yu, Young Suk; Kim, Dong Hun; Min, Bon-Hong; Kim, Kyu-Won

    2007-12-01

    In spite of recent advances in the treatment of retinoblastoma, chemotherapy is still challenging in high-stage intraocular retinoblastoma or metastatic retinoblastoma. Here, we investigated whether arginine deprivation via arginine deiminase (ADI) could be a new anti-tumor therapy in retinoblastoma cells. Expression of argininosuccinate synthetase (ASS) was detected in human retinoblastoma tissues. Even with a high expression of ASS, ADI effectively inhibited the proliferation of retinoblastoma cells and induced retinoblastoma cell death in a dose-dependent manner. These results indicate that arginine deprivation via ADI could be another treatment option for retinoblastoma due to low ASS activity in retinoblastoma cells.

  7. La neuroplasticidad aplicada al deporte

    OpenAIRE

    Fernández, Hernán

    2015-01-01

    Me decidí a realizar la siguiente ponencia, para analizar y reconocer la importancia que implica el trabajo cognitivo en el entrenamiento deportivo. El abordaje que efectuaré, está enfocado en el desarrollo del entrenamiento cognitivo, su fundamento y el desarrollo de la neuroplasticidad. Analizaré el concepto de neuroplasticidad y neurogénesis como base al sustento teórico de la neurociencia aplicada al deporte. También remarcaré diversas metodologías prácticas, con su fundamento teóri...

  8. Review of Retinoblastoma and Report of Three Cases of Bilateral Retinoblastoma

    Directory of Open Access Journals (Sweden)

    M Kheirandish

    2009-01-01

    Full Text Available Retinoblastoma is a retinal malignant tumor that occurs in early childhood. The annual incidence of retinoblastoma is approximately one per 14 000 live births. 250 to 300 new cases are reported in the United States each year. It develops due to inactivation of both alleles of the retinoblastoma susceptibility (RB1 gene. All hereditary cases (55% are with bilateral and multifocal lesions, but in non hereditary cases (45%, retinoblastoma is always unilateral and unifocal. Leukocoria (white reflection of pupil and strabismus are the most frequent clinical manifestations of retinoblastoma. In funduscopy under general anesthesia, the lesion appears as a white tumor with angiomatous dilatation of the vessels. Treatment approaches include chemotherapy, focal laser, enucleation and radiotherapy. The goals of treatment are eye preservation and improvement in patient survival rate; reduction of local tumor relapse and recurrence and development of new tumors. Follow up is by general and complete eye examination in order to diagnose related malignancies, especially in cases of radiotherapy. We report three bilateral retinoblastoma cases (10 months, 5 and6 years old with strabismus and visual loss. Imaging showed involvement of both globes without CNS involvement. The tumor was controlled by providing adequate treatment for the cases

  9. Website: material de apoio para professores de biofísica aplicada a enfermagem

    OpenAIRE

    Guimarães,Fábio Sander Prado; Dickman,Adriana Gomes; Chaves, Andréa Carla Leite

    2014-01-01

    Esse trabalho relata o processo de elaboração de um website relacionando a física com a biologia com o objetivo de instrumentalizar o professor para o ensino de biofísica aplicada ao curso de enfermagem. O material inclui compilações de textos, exercícios, vídeos, animações, situações problematizadoras e artigos científicos sobre biofísica aplicada à enfermagem. Os temas abordados foram definidos a partir de uma pesquisa realizada junto a enfermeiros, professores e alunos do curso de enfermag...

  10. Guidelines for imaging retinoblastoma: imaging principles and MRI standardization

    Energy Technology Data Exchange (ETDEWEB)

    Graaf, Pim de; Rodjan, Firazia; Castelijns, Jonas A. [VU University Medical Center, Department of Radiology, Amsterdam (Netherlands); Goericke, Sophia [University Hospital, Department of Diagnostic and Interventional Radiology and Neuroradiology, Essen (Germany); Galluzzi, Paolo [Azienda Ospedaliera e Universitaria Senese, Policlinico ' ' Le Scotte' ' , Unit of Diagnostic and Therapeutic Neuroradiology, Siena (Italy); Maeder, Philippe [CHUV, Service de Radiodiagnostic et Radiologie Interventionelle, Lausanne (Switzerland); Brisse, Herve J. [Institut Curie, Departement d' Imagerie, Paris (France)

    2012-01-15

    Retinoblastoma is the most common intraocular tumor in children. The diagnosis is usually established by the ophthalmologist on the basis of fundoscopy and US. Together with US, high-resolution MRI has emerged as an important imaging modality for pretreatment assessment, i.e. for diagnostic confirmation, detection of local tumor extent, detection of associated developmental malformation of the brain and detection of associated intracranial primitive neuroectodermal tumor (trilateral retinoblastoma). Minimum requirements for pretreatment diagnostic evaluation of retinoblastoma or mimicking lesions are presented, based on consensus among members of the European Retinoblastoma Imaging Collaboration (ERIC). The most appropriate techniques for imaging in a child with leukocoria are reviewed. CT is no longer recommended. Implementation of a standardized MRI protocol for retinoblastoma in clinical practice may benefit children worldwide, especially those with hereditary retinoblastoma, since a decreased use of CT reduces the exposure to ionizing radiation. (orig.)

  11. Atraso diagnóstico do retinoblastoma

    OpenAIRE

    2004-01-01

    OBJETIVOS: Identificar os principais sinais e sintomas do retinoblastoma e determinar o tempo médio entre o início dos sintomas e o diagnóstico. MÉTODOS: Estudo retrospectivo, com revisão dos prontuários das crianças diagnosticadas com retinoblastoma entre janeiro de 1991 e junho de 2000 no Departamento de Pediatria do Hospital do Câncer de São Paulo. Análise estatística: teste t de Student, análise e variância, teste de Tukey-HSD (honest significant differences), teste de Levene, análise de ...

  12. Imaging in the trilateral retinoblastoma syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Bagley, L.J. [Department of Radiology, Hospital of the University of Pennsylvania, 3400 Spruce St., Philadelphia, PA 19104 (United States); Hurst, R.W. [Department of Radiology, Hospital of the University of Pennsylvania, 3400 Spruce St., Philadelphia, PA 19104 (United States); Zimmerman, R.A. [Department of Radiology, Children`s Hospital of Philadelphia, 34 th St. and Civic Center Blvd., Philadelphia, PA 19104 (United States); Shields, J.A. [Ocular Oncology Service, Wills Eye Hospital, 900 Walnut St., Philadelphia, PA 19107 (United States); Shields, C.L. [Ocular Oncology Service, Wills Eye Hospital, 900 Walnut St., Philadelphia, PA 19107 (United States); De Potter, P. [Ocular Oncology Service, Wills Eye Hospital, 900 Walnut St., Philadelphia, PA 19107 (United States)

    1996-02-01

    The medical records, CT, and MRI of ten children with trilateral retinoblastoma were reviewed. The intracranial pathology consisted of eight pineal neoplasms and two parasellar lesions, at least seven of the which were calcified. Two lesions demonstrated calcification only (no soft tissue mass) at initial presentation. Hydrocephalus was seen in eight cases, and concurrent or subsequent subarachnoid dissemination was documented in seven. Only one patient is known to be alive at the present time. The imaging features of the midline intracranial tumors mirror those of the ocular neoplasm. As calcification may be the only clue to the presence of the intracranial malignancy, close surveillance of high-risk patients with retinoblastoma with initial CT and follow-up MRI is suggested. (orig.). With 3 figs., 1 tab.

  13. Radiation management of retinoblastoma; An analysis

    Energy Technology Data Exchange (ETDEWEB)

    Takemasa, Kazuhiko; Ito, Hisao; Hashimoto, Shozo; Tanaka, Yasuhiko; Oguchi, Yoshihisa (Keio Univ., Tokyo (Japan). School of Medicine)

    1991-12-01

    An analysis has been conducted of 45 patients treated for retinoblastoma at Keio University Hospital between 1970 and 1990. Of these patients, 32 had unilateral lesion and 13 had bilateral lesion. Further, since their disease was far advanced, 29 patients with unilateral lesion and 12 patients with bilateral lesion underwent enucleation. As a result, 3 patients with unilateral retinoblastoma and all patients with bilateral manifestation of the disease were treated with radiotherapy (45-50 Gy) with or without cryotherapy and photocoagulation. One patient with unilateral lesion, who had received both radiotherapy and chemotherapy, showed metastases at the first presentation at our clinic and thus was excluded from this analysis. Among 16 eyes of 15 patients who were given radiotherapy, 6 eyes developed recurrence and needed to have further treatment. In 6 eyes out of 12, cataract developed, and out of 10 eyes in which eye function was evaluable, good vision was able to be preserved in 5 eyes. (author).

  14. Immunohistochemical demonstration of glial markers in retinoblastomas

    DEFF Research Database (Denmark)

    Schrøder, H D

    1987-01-01

    Twenty retinoblastomas were studied immunohistochemically in order to visualize glial cells. In the retina, the glial cells in the ganglion cell layer and the Müller cells were GFAP positive, while only the glial cells of the ganglion cell layer expressed S-100 reactivity. In the tumours S-100/GFAP...... positive glial cells were found in areas near the retina and along many tumour vessels. Some S-100 reactive cells previously interpreted as tumour cells were refound in a few tumours. In areas with Flexner-Winterstein rosettes and in areas with light cells showing photoreceptor-like differentiation, glial...... cells reactive for both S-100 and GFAP were demonstrated. The latter findings may represent differentiation in a glial direction in the more mature parts of retinoblastoma....

  15. Diffuse infiltrating retinoblastoma invading subarachnoid space

    Directory of Open Access Journals (Sweden)

    Kase S

    2011-06-01

    Full Text Available Satoru Kase1, Kazuhiko Yoshida1, Shigenobu Suzuki2, Koh-ichi Ohshima3, Shigeaki Ohno4, Susumu Ishida11Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo; 2Department of Ophthalmic Oncology, National Cancer Center Hospital, Tokyo; 3Section of Ophthalmology, Okayama Medical Center, Okayama; 4Department of Ocular Inflammation and Immunology, Hokkaido University Graduate School of Medicine, Sapporo, JapanAbstract: We report herein an unusual case of diffuse infiltrating retinoblastoma involving the brain, which caused a patient’s death 27 months after enucleation. An eight-year-old boy complained of blurred vision in his right eye (OD in October 2006. Funduscopic examination showed optic disc swelling, dense whitish vitreous opacity, and an orange-colored subretinal elevated lesion adjacent to the optic disc. Fluorescein angiography revealed hyperfluorescence in the peripapillary region at an early-phase OD. Because the size of the subretinal lesion and vitreous opacity gradually increased, he was referred to us. His visual acuity was 20/1000 OD on June 20, 2007. Slit-lamp biomicroscopy showed a dense anterior vitreous opacity. Ophthalmoscopically, the subretinal orange-colored area spread out until reaching the mid peripheral region. A B-mode sonogram and computed tomography showed a thick homogeneous lesion without calcification. Gadolinium-enhanced magnetic resonance imaging showed a markedly enhanced appearance of the underlying posterior retina. Enucleation of the right eye was performed nine months after the initial presentation. Histopathology demonstrated retinal detachment and a huge choroidal mass invading the optic nerve head. The tumor was consistent with diffuse infiltrating retinoblastoma. The patient died due to brain involvement 27 months after enucleation. Ophthalmologists should be aware that diffuse infiltrating retinoblastoma may show an unfavorable course if its diagnosis is delayed

  16. MicroRNAs horizon in retinoblastoma.

    Directory of Open Access Journals (Sweden)

    Mojgan Mirakholi

    2013-12-01

    Full Text Available In the retinoblastoma research, it is of great interest to identify molecular markers associated with the genetics of tumorigenesis. microRNAs (miRNAs are small non-coding RNA molecules that play a regulatory role in many crucial cellular pathways such as differentiation, cell cycle progression, and apoptosis. A body of evidences showed dysregulation of miRNAs in tumor biology and many diseases. They potentially play a significant role in tumorigenesis processes and have been the subject of research in many types of cancers including retinal tumorigenesis. miRNA expression profiling was found to be associated with tumor development, progression and treatment. These associations demonstrate the putative applications of miRNAs in monitoring of different aspect of tumors consisting diagnostic, prognostic and therapeutic. Herein, we review the current literature concerning to the study of miRNA target recognition, function to tumorigenesis and treatment in retinoblastoma. Identification the specific miRNA biomarkers associated with retinoblastoma cancer may help to establish new therapeutic approaches for salvage affected eyes in patients.

  17. Transcriptional inhibition by the retinoblastoma protein

    DEFF Research Database (Denmark)

    Fattaey, A; Helin, K; Harlow, E

    1993-01-01

    The retinoblastoma protein, pRB, appears to play a key role in coordinating the regulation of cell cycle position and transcriptional events. pRB undergoes specific cell-cycle-dependent phosphorylation, being underphosphorylated in G1 and heavily phosphorylated in S, G2, and M. The underphosphory......The retinoblastoma protein, pRB, appears to play a key role in coordinating the regulation of cell cycle position and transcriptional events. pRB undergoes specific cell-cycle-dependent phosphorylation, being underphosphorylated in G1 and heavily phosphorylated in S, G2, and M....... The underphosphorylated form is able to interact with the E2F transcription factor. Recently, we have cloned a cDNA for E2F-1. By using this clone and a series of non-pRB binding mutants, we have been able to show that the binding of pRB to E2F-1 causes inhibition of E2F-mediated transactivation. pRB's inhibition of E2F......-mediated transcription would be lost by mutation in the retinoblastoma gene in human tumours, by pRB's interaction with DNA tumour virus oncoproteins, or by phosphorylation during the cell cycle....

  18. Toxicología General y Aplicada

    OpenAIRE

    García Fernández, Antonio Juan

    2011-01-01

    Presentaciones de los temas de Toxicología General y Aplicada: clínica, alimentaria y ambiental impartidos en la asignatura de Toxicología de la licenciatura de Veterinaria en la Universidad de Murcia en el curso 2011/12. Presentaciones de los temas de Toxicología General y Aplicada: clínica, alimentaria y ambiental impartidos en la Facultad de Veterinaria en el curso 2011/12.

  19. Tratamiento conservador en pacientes con retinoblastoma bilateral

    Directory of Open Access Journals (Sweden)

    Juan C. Suárez

    2008-11-01

    Full Text Available OBJETIVO: comparar el tratamiento convencional del retinoblastoma bilateral, usado hasta hace algunos años, consistente en radioterapia o enucleación bilateral, con el tratamiento conservador actual que incluye termoterapia transpupilar (TTT o TTT/quimioterapia al menos en un ojo, en niños con diagnóstico de retinoblastoma bilateral. DISEÑO: estudio retrospectivo descriptivo. MUESTRA: 20 pacientes con diagnóstico de retinoblastoma bilateral que consultaron al Hospital Universitario San Vicente de Paúl, de Medellín, Colombia, entre 1997 y 2007. MÉTODO: se hizo enucleación del ojo con el tumor de mayor tamaño. En el otro ojo se hizo tratamiento con TTT, con el láser diodo (810 nm, spot amplio, solo o combinado con otras terapias. RESULTADOS: se dividió a los pacientes en dos grupos: 16 pacientes (32 ojos en el grupo 1 tratados conservadoramente y 4 pacientes (8 ojos en el grupo 2 con tratamiento convencional. El rango de edad fue de 1-72 meses en el grupo 1 y de 1-12 meses en el grupo 2. El tiempo de seguimiento fue de 7-67 meses para el grupo 1 y de 13-73 meses para el grupo 2. En el grupo 1 se hizo enucleación de 16 ojos (50%, radioterapia externa de uno (3,1%, quimioterapia más termoterapia de 5 (15,6% y quimioterapia más termoterapia más crioterapia de 10 (31,3%. En todos los pacientes se logró preservar al menos un ojo. En el grupo 2, se enuclearon 7 ojos (87,5% y se hizo radioterapia externa más enucleación en un paciente (12.5%. Además, todos los pacientes recibieron quimioterapia. CONCLUSIÓN: la terapia conservadora actual consistente en tratamiento local (termoterapia, crioterapia o braquiterapia y quimiorreducción permite preservar al menos un ojo y en algunos casos de los dos, muchas veces con buena agudeza visual, en niños con retinoblastoma bilateral; se evitan así la enucleación bilateral y la radioterapia externa usada en el tratamiento convencional con todos sus efectos secundarios. La enucleación contin

  20. Cancer incidence after retinoblastoma - Radiation dose and sarcoma risk

    NARCIS (Netherlands)

    Wong, FL; Boice, JD; Abramson, DH; Tarone, RE; Kleinerman, RA; Stovall, M; Goldman, MB; Seddon, JM; Tarbell, N; Fraumeni, JF; Li, FP

    1997-01-01

    Context.-There is a substantial risk of a second cancer for persons with hereditary retinoblastoma, which is enhanced by radiotherapy. Objective.-To examine long-term risk of new primary cancers in survivors of childhood retinoblastoma and quantify the role of radiotherapy in sarcoma development. De

  1. Retinoblastoma bilateral de aparecimento tardio: relato de caso Late presentation of bilateral retinoblastoma: case report

    Directory of Open Access Journals (Sweden)

    Maria Cecília Santos Cavalcanti Melo

    2008-06-01

    Full Text Available É relatado um caso de retinoblastoma de aparecimento tardio, com características pouco freqüentes, com o objetivo de melhorar o controle da afecção. Em 1993, SJMMF, nove meses, masculino, leucodermo, apresentou tumor na retina esquerda. O paciente tinha história familiar de retinoblastoma. A enucleação foi realizada, comprovando-se retinoblastoma. Os exames para estadiamento sistêmico foram normais. O olho direito evoluiu normal por dez anos. Em exame de rotina, foram constatadas três lesões de retinoblastoma na retina nasal. Foi feita termoterapia transpupilar, com laser de diodo de 810 nm. Após 30 dias, as lesões regrediram. Após 60 dias houve recidiva na borda da lesão, onde foi realizada crioterapia transescleral, com regressão do tumor por seis meses. Durante o controle, observaram-se condensações próximas à lesão tumoral atrófica (sementes vítreas. Foi feito braquiterapia com Iodo125, havendo desaparecimento das mesmas após 30 dias. Novas sementes surgiram três meses pós-braquiterapia, depositadas na superfície retiniana, sendo tratadas com crioterapia transescleral e termoterapia transpupilar, havendo regressão. O paciente evoluiu com nova semente vítrea após seis meses, a qual, após depositar-se na superfície da retina, foi tratada com termoterapia transpupilar. Está em seguimento há 38 meses desde o aparecimento do tumor bilateral, mantendo acuidade visual de 20/20 e exames clínicos normais. Considera-se importante este caso pela pouca freqüência de aparecimento da doença nesta idade. Julga-se necessário o alerta para os casos de retinoblastoma já considerados curados.A case of retinoblastoma with uncommon features is reported, aiming at improving follow-up. In 1993, SJMMF, 9-month-old white boy, presented a squint in the left eye. A retinal tumor was detected. The patient had a family history of retinoblastoma. Enucleation was performed and retinoblastoma was proved. The patient underwent

  2. Trilateral retinoblastoma with unilateral eye involvement

    Directory of Open Access Journals (Sweden)

    Gabriel Costa de Andrade

    2015-08-01

    Full Text Available SummaryRetinoblastomas (RB are the main forms of intraocular tumor in childhood, with a worldwide incidence of 1 case per 15,000 to 20,000 live births. Trilateral RB (RBT is a rare combination of unilateral or bilateral RB with a midline intracranial neoplasm of neuroblastic origin, usually found in the pineal region or the suprasellar region, presenting variable incidence of 0.5% up to 6% among patients with RB. The article reports a case of unilateral RBT in a patient treated at Hospital A.C.Camargo.

  3. The retinoblastoma protein as a transcriptional repressor

    DEFF Research Database (Denmark)

    Helin, K; Ed, H

    1993-01-01

    The retinoblastoma protein (pRB) is one of the best-studied tumour suppressor gene products. Its loss during the genesis of many human tumours, its inactivation by several DNA tumour virus oncoproteins, and its ability to inhibit cell growth when introduced into dividing cells all suggest that pRB...... negatively regulates some aspect of normal cell growth. The discovery that pRB associates with transcription factors such as E2F has provided the first model for pRB function. In this review, we discuss how pRB may regulate cell growth by repressing transcription of genes essential for cell proliferation....

  4. Clinical Value of CD24 Expression in Retinoblastoma

    Directory of Open Access Journals (Sweden)

    Jia Li

    2012-01-01

    Full Text Available Background. The expression of CD24 has been detected in a wide variety of human malignancies. Downregulation of CD24 inhibits proliferation and induces apoptosis in tumor cells, whereas its upregulation increases tumor growth and metastasis. However, no data on CD24 protein levels in retinoblastoma are available, and the mechanism of CD24 involvement in retinoblastoma progress has not been elucidated. The aim of this study was to explore the expression profile of CD24 in the retinoblastoma tumor samples and to correlate with clinicopathological parameters. Methods. Immunohistochemistry was performed for CD24 on the archival paraffin sections of retinoblastoma and correlated with clinicopathological features. Western blotting was performed to confirm immunoreactivity results. Results. CD24 immunoreactivity was observed in 72.0% (36/50 of the retinoblastoma specimens. Among the 35 low-risk tumors, CD24 was expressed in 62.9% (22/35 tumors and among the 15 high-risk tumors, CD24 was expressed in 93.3% (14/15 tumors. High-risk tumors showed significantly increased expression of CD24 compared to tumors with low-risk (<0.05. Conclusions. This is the first correlation between CD24 expression and histopathology in human retinoblastoma. Our study showed increased expression of CD24 in high risk tumors compared to low risk tumors. Further functional studies are required to explore the role of CD24 in retinoblastoma.

  5. Notas sobre a segunda avaliação externa do programa de treinamento em epidemiologia aplicada aos serviços do sistema único de saúde do Brasil - EPISUS: potencialidades do enfoque qualitativo-participativo Notes on the second external evaluation of the training program in epidemiology applied to the services of brazil's national health system - EPISUS: potentialities of the qualitative-participatory approach

    Directory of Open Access Journals (Sweden)

    Maria Lúcia Magalhães Bosi

    2009-09-01

    Full Text Available Este trabalho objetiva relatar o emprego do enfoque qualitativo-participativo, bem como discutir seus fundamentos e potencialidades, tomando como base a participação dos autores na segunda avaliação externa do Programa de Treinamento em Epidemiologia Aplicada aos Serviços do Sistema Único de Saúde - EPISUS. Finalizada em outubro de 2007, em parceria com a Secretaria de Vigilância em Saúde e o Center for Diseases Control (CDC/Atlanta, a referida avaliação incorporou o enfoque da chamada quarta geração (fourth generation evaluation, no qual os avaliadores operam como mediadores, substituindo, assim, os fundamentos do enfoque tradicional antes utilizado. Para tanto, tornou-se necessária uma demarcação conceitual referente aos conceitos avaliação, qualitativo e participativo que orientariam o processo avaliativo focalizado. Quanto ao conceito avaliação, sua natureza conflui para as propostas de quarta geração e, portanto, transita de um caráter punitivo para um caráter construtivo. A dimensão participativa aponta para diferentes sentidos do que seja participar e, na experiência aqui relatada, adotou-se o sentido decisório, onde se busca reverter assimetrias de poder. O qualitativo é concebido na interface com a subjetividade, referindo-se a informações que não se submetem à quantificação. Tal modelo permitiu desvelar aspectos que, muitas vezes, se ocultam nos números e nas generalizações abstratas, tornando possível focalizar as relações que constituem o cotidiano dos programas e práticas em saúde, subsidiando, assim, sua transformação.This paper aims to present the authors' experience concerning the use of the qualitative-participatory approach in the second external evaluation of the Training Program in Epidemiology Applied to the Services of the National Health System - EPISUS. Completed in October 2007, in partnership with the Health Surveillance Department and the Center for Diseases Control (CDC

  6. Distant metastatic retinoblastoma without central nervous system involvement

    Directory of Open Access Journals (Sweden)

    Mohammad Javed Ali

    2013-01-01

    Full Text Available Retinoblastoma is the most common intraocular malignancy in children, with a reported incidence ranging from 1 in 15,000 to 1 in 18,000 live births. Metastatic retinoblastoma is rare in developed countries, with a reported range from 4.8% in the United States to 5.8% in the United Kingdom. However, the frequency reported from developing countries varies from 9 to 11% at presentation. The mortality is very high owing to late presentations, delayed diagnosis compounded by socio-economic factors. The management of metastatic retinoblastoma is evolving, but it is still a challenge in pediatric oncology. We present a case of an extensive skeletal metastasis that initially presented as a massive orbital retinoblastoma.

  7. Array comparative genomic hybridization in retinoma and retinoblastoma tissues.

    Science.gov (United States)

    Sampieri, Katia; Amenduni, Mariangela; Papa, Filomena Tiziana; Katzaki, Eleni; Mencarelli, Maria Antonietta; Marozza, Annabella; Epistolato, Maria Carmela; Toti, Paolo; Lazzi, Stefano; Bruttini, Mirella; De Filippis, Roberta; De Francesco, Sonia; Longo, Ilaria; Meloni, Ilaria; Mari, Francesca; Acquaviva, Antonio; Hadjistilianou, Theodora; Renieri, Alessandra; Ariani, Francesca

    2009-03-01

    In retinoblastoma, two RB1 mutations are necessary for tumor development. Recurrent genomic rearrangements may represent subsequent events required for retinoblastoma progression. Array-comparative genomic hybridization was carried out in 18 eye samples, 10 from bilateral and eight from unilateral retinoblastoma patients. Two unilateral cases also showed areas of retinoma. The most frequent imbalance in retinoblastomas was 6p gain (40%), followed by gains at 1q12-q25.3, 2p24.3-p24.2, 9q22.2, and 9q33.1 and losses at 11q24.3, 13q13.2-q22.3, and 16q12.1-q21. Bilateral cases showed a lower number of imbalances than unilateral cases (P = 0.002). Unilateral cases were divided into low-level ( or = 7) chromosomal instability groups. The first group presented with younger age at diagnosis (mean 511 days) compared with the second group (mean 1606 days). In one retinoma case ophthalmoscopically diagnosed as a benign lesion no rearrangements were detected, whereas the adjacent retinoblastoma displayed seven aberrations. The other retinoma case identified by retrospective histopathological examination shared three rearrangements with the adjacent retinoblastoma. Two other gene-free rearrangements were retinoma specific. One rearrangement, dup5p, was retinoblastoma specific and included the SKP2 gene. Genomic profiling indicated that the first retinoma was a pretumoral lesion, whereas the other represents a subclone of cells bearing 'benign' rearrangements overwhelmed by another subclone presenting aberrations with higher 'oncogenic' potential. In summary, the present study shows that bilateral and unilateral retinoblastoma have different chromosomal instability that correlates with the age of tumor onset in unilateral cases. This is the first report of genomic profiling in retinoma tissue, shedding light on the different nature of lesions named 'retinoma'.

  8. Scleral buckling for retinal detachment in patients with retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Buzney, S.M.; Pruett, R.C.; Regan, C.D.; Walton, D.S.; Smith, T.R.

    1984-10-15

    Three children (two girls and one boy) with bilateral retinoblastoma each developed a presumed rhegmatogenous retinal detachment in one eye. All three eyes had previously received radiation and cryotherapy. In each case the retinal detachment responded promptly to conventional surgical methods via scleral buckling in the area of treated retinoblastoma and presumed retinal break. All three eyes have retained useful vision for follow-up periods of 3.5 to 12 years.

  9. Retinoblastoma protein: a central processing unit.

    Science.gov (United States)

    Poznic, M

    2009-06-01

    The retinoblastoma protein (pRb) is one of the key cell-cycle regulating proteins and its inactivation leads to neoplastic transformation and carcinogenesis. This protein regulates critical G1 -to-S phase transition through interaction with the E2F family of cell-cycle transcription factors repressing transcription of genes required for this cell-cycle check-point transition. Its activity is regulated through network sensing intracellular and extracellular signals which block or permit phosphorylation (inactivation) of the Rb protein. Mechanisms of Rb-dependent cell-cycle control have been widely studied over the past couple of decades. However, recently it was found that pRb also regulates apoptosis through the same interaction with E2F transcription factors and that Rb-E2F complexes play a role in regulating the transcription of genes involved in differentiation and development.

  10. CT diagnosis of retinoblastoma with histopathologic correlations

    Energy Technology Data Exchange (ETDEWEB)

    Olivecrona, H. [Dept. of Radiology, King Khaled Eye Specialist Hospital, Riyadh (Saudi Arabia); Agerberg, P.A. [Dept. of Radiology, King Khaled Eye Specialist Hospital, Riyadh (Saudi Arabia); Huaman, A. [Dept. of Pathology, King Khaled Eye Specialist Hospital, Riyadh (Saudi Arabia)

    1994-08-01

    The authors correlated the radiologic and histopathologic characteristics of 108 retinoblastomas in 98 patients. Calcifications were demonstrable by CT scans in 92.6% of the cases, whereas only 82% were evident upon routine histologic study. Cases of severe invasion of the optic nerve beyond the lamina cribrosa and up to the surgical margin of the nerve were detected by CT scans. Lesser degrees of invasion could not be determined by the same method. Orbital extension was also demonstrable, whereas invasion limited to the sclera was rarely accessible to CT diagnosis. Choroidal invasion was not detectable. Computerized tomography detected enlargement of the ipsilateral lacrimal gland in 17.5% of patients. This phenomenon correlates with invasion of the choroid and the optic nerve. (orig.)

  11. Retinoblastoma protein: a central processing unit

    Indian Academy of Sciences (India)

    M Poznic

    2009-06-01

    The retinoblastoma protein (pRb) is one of the key cell-cycle regulating proteins and its inactivation leads to neoplastic transformation and carcinogenesis. This protein regulates critical G1-to-S phase transition through interaction with the E2F family of cell-cycle transcription factors repressing transcription of genes required for this cell-cycle check-point transition. Its activity is regulated through network sensing intracellular and extracellular signals which block or permit phosphorylation (inactivation) of the Rb protein. Mechanisms of Rb-dependent cell-cycle control have been widely studied over the past couple of decades. However, recently it was found that pRb also regulates apoptosis through the same interaction with E2F transcription factors and that Rb–E2F complexes play a role in regulating the transcription of genes involved in differentiation and development.

  12. Cobalt60 plaques in recurrent retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Fass, D.; McCormick, B.; Abramson, D.; Ellsworth, R. (Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, NY, NY (USA))

    1991-08-01

    Cobalt60 plaque irradiation is one treatment option for patients with recurrent retinoblastoma following conventional external beam irradiation (ERT). Tumorocidal doses can be delivered without excessive risk of normal tissue injury. In patients not considered candidates for xenon arc or cryotherapy, 60Co is an alternative to enucleation. Between 1968 and 1987, 85 patients were treated with 60Co plaques, 72 of whom had failed prior ERT. Age at diagnosis ranged from 1 week to 4 years. There are 37 males and 35 females. Seventy-one patients had bilateral disease and one had unilateral. Three patients had both eyes plaqued. Prior ERT ranged from 30 to 70 Gy (mean 4200 Gy). Time from initial therapy to failure ranged from 13 to 60 months. Cobalt plaques of 10 mm, 15 mm, or 10 {times} 15 mm were used depending on tumor size and location. Dose prescribed to the apex of the tumor ranged from 30 to 50 Gy (median 40 Gy) given over 3 to 8 days. Twelve patients had two plaque applications; three patients had three plaque applications. All patients were followed with routine ophthalmoscopic examinations. Follow-up ranged from 2 to 22 years (mean 8.7). Seven patients died of metastatic disease; 10 patients developed non-ocular second tumors. Thirty patients required enucleation. Twenty-two patients had clear tumor progression, two patients had radiation complications, and six patients had a combination of tumor growth and complications. Cobalt60 can salvage eyes in retinoblastoma patients failing ERT. Currently, the authors are using I125 in an attempt to spare normal ocular tissue and reduce subsequent complications.

  13. Retinoma and Phthisis Bulbi of Retinoblastoma 1.Clinical and Genetic Analysis

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    Retinoma and phthisis bulbi of retinoblastoma are rare entitiesfound in retinoblastoma patients and their relatives.Eleven cases of phthisisbulbi of retinoblastoma and 9 cases of retinoma were identified from 1966 to1991 in our center.The clinic data show that retinoma and phthisis bulbi areclosely related to the retinoblastoma gene.Enucleation should be carried outas soon as possible without hesitation for the phthisis of eyes with retinoblas-toma.Genetic counseling and frequent observation should be p...

  14. Eficiência térmica de telhas onduladas de fibrocimento aplicadas em abrigos individuais para bezerros expostos ao sol e à sombra Thermal efficiency of fiber cement corrugated sheets applied to individual housing for calves exposed to sun and shade

    Directory of Open Access Journals (Sweden)

    Juliano Fiorelli

    2012-01-01

    Full Text Available Este trabalho apresenta um estudo da eficiência térmica de coberturas de bezerreiros individuais expostas ao sol e à sombra, por meio de termografia infravermelha, temperatura interna e índices de conforto térmico. Foram avaliados quatro bezerreiros, três expostos ao sol, a saber: (i cobertos com telhas de fibrocimento sem amianto - pintadas de branco, (ii sem pintura e (iii com sombrite posicionado internamente aos bezerreiros, à distância de 0,10m da face inferior da telha. O quarto bezerreiro foi instalado em área sombreada e coberto com telhas de fibrocimento sem pintura. As coletas de dados foram realizadas durante 21 dias, nos horários das 11h00min, 14h00min e 17h00min. Os resultados mostraram variações significativas na temperatura de superfície das coberturas e nos índices de conforto térmico, entre os tratamentos expostos ao sol e à sombra, para todos os horários avaliados. As imagens termográficas infravermelhas mostraram-se eficientes para melhor compreensão dos processos de transferência de calor da cobertura para o interior das instalações.This research presents a study of roof thermal efficiency in individual housing for calves exposed to sun and shade through infrared thermography, internal temperature and thermal comfort indexes. Four different individual housing for calves covered with asbestos-free fiber-cement corrugated sheets were evaluated. Three of them were directly exposed to the sun: (i corrugated sheets painted white in the external surface, (ii corrugated sheets without painting and (iii with screen shade fabric installed 0.10m under de internal surface of the corrugated sheet. The fourth individual housing was installed in the shade area and covered with unpainted corrugated fiber-cement sheets. The analysis was taken for 21 days at 11h00min, 14h00min and 17h00min. The results indicate significant variations in the roofing surface temperature and thermal comfort indexes among the treatments

  15. Design and Implementation of the Retinoblastoma Collaborative Laboratory

    Science.gov (United States)

    Qaiser, Seemi; Limo, Alice; Gichana, Josiah; Kimani, Kahaki; Githanga, Jessie; Waweru, Wairimu; Dimba, Elizabeth A.O.; Dimaras, Helen

    2017-01-01

    Purpose The purpose of this work was to describe the design and implementation of a digital pathology laboratory, the Retinoblastoma Collaborative Laboratory (RbCoLab) in Kenya. Method The RbCoLab is a central lab in Nairobi that receives retinoblastoma specimens from all over Kenya. Specimens were processed using evidence-based standard operating procedures. Images were produced by a digital scanner, and pathology reports were disseminated online. Results The lab implemented standard operating procedures aimed at improving the accuracy, completeness, and timeliness of pathology reports, enhancing the care of Kenyan retinoblastoma patients. Integration of digital technology to support pathology services supported knowledge transfer and skills transfer. A bidirectional educational network of local pathologists and other clinicians in the circle of care of the patients emerged and served to emphasize the clinical importance of cancer pathology at multiple levels of care. A ‘Robin Hood’ business model of health care service delivery was developed to support sustainability and scale-up of cancer pathology services. Discussion The application of evidence-based protocols, comprehensive training, and collaboration were essential to bring improvements to the care of retinoblastoma patients in Kenya. When embraced as an integrated component of retinoblastoma care, digital pathology offers the opportunity for frequent connection and consultation for development of expertise over time. PMID:28275608

  16. Outcomes of Cataract Surgery Following Treatment for Retinoblastoma

    Science.gov (United States)

    Kim, Hyeong Min; Lee, Byung Joo; Kim, Jeong Hun

    2017-01-01

    Purpose To evaluate the long-term visual outcomes and complications of cataract surgery in eyes previously treated for retinoblastoma. Methods We reviewed the medical records of patients who underwent cataract extraction and intraocular lens implantation at Seoul National University Children's Hospital for a secondary cataract that developed after retinoblastoma treatment. Results During the period between 1990 and 2014, 208 eyes of 147 patients received eye-salvaging treatment (radiotherapy, chemotherapy, and local therapy) for retinoblastoma at Seoul National University Children's Hospital. Among these eyes, a secondary cataract was detected in 17 eyes of 14 patients, and five eyes of five patients underwent cataract surgery. The median age of cataract formation was 97 months (range, 38 to 153 months). The medial interval between the diagnosis of retinoblastoma and cataract formation was 79 months (range, 29 to 140 months). All patients received posterior chamber intraocular lens insertion after irrigation and aspiration of the lens through a scleral tunnel incision. Anterior vitrectomy and posterior capsulotomy were performed in two eyes and a laser capsulotomy was subsequently performed in one eye. No intraoperative and postoperative complications occurred. The median follow-up after surgery was 36 months (range, 14 to 47 months). The final best corrected visual acuities were improved in all five eyes. No intraocular tumor recurrences or metastases occurred. Conclusions After retinoblastoma regression, cataract extraction in our series was not associated with tumor recurrence or metastasis. Visual improvement was noted in every patient. PMID:28243024

  17. The role of intravitreal chemotherapy for retinoblastoma

    Directory of Open Access Journals (Sweden)

    Fairooz P Manjandavida

    2015-01-01

    Full Text Available Targeted therapy in retinoblastoma (RB is widely accepted as the current management tool with an aim of increasing drug availability at the tumor location. Inevitably the effect is several times higher compared to systemic delivery of chemotherapeutic drugs and carries less systemic toxicity. Despite tremendous advancement in saving life, eye salvage in advanced RB especially with active vitreous seeds remains a challenge. The hypoxic environment of the vitreous and reduced vitreous concentration of the drugs delivered makes these tumor seeds resistant to chemotherapy. Direct delivery of chemotherapeutic drugs into the vitreous cavity aids to overcome these challenges and is progressively being accepted worldwide. However, intraocular procedure in RB was abandoned due to high risk of extraocular tumor dissemination. Recently, the forbidden therapeutic technique was re-explored and modified for safe use. Although eye salvage rate has tremendously improved after intravitreal chemotherapy (IVitC, retinal toxicity, and vision salvage are yet to be validated. In our preliminary report of intravitreal melphalan in 11 eyes, we reported 100% eye salvage and 0% recurrence with an extended 15 months mean follow-up. In this review, we analyzed published reports on IVitC in RB via PubMed, Medline, and conference proceedings citation index, electronic database search, without language restriction that included case series and reports of humans and experimental animal eyes with RB receiving IVitC.

  18. Current Trends in the Management of Retinoblastoma

    Directory of Open Access Journals (Sweden)

    Hayyam Kıratlı

    2014-09-01

    Full Text Available Retinoblastoma is the most common primary intraocular malignant tumor of early childhood, and 95% of all cases are diagnosed before the age of 5 years. Tumors are evaluated and classified based on laterality, genetic features, the number, location and size of foci. Currently, systemic and intaarterial chemotherapy are the most commonly used management options worldwide. Group A tumors are treated with focal techniques including transpupillary thermotherapy, cryotherapy, or laser photocoagulation. Groups B and C tumors are managed with systemic chemotherapy and focal consolidation techniques. Group D lesions are best treated with systemic chemotherapy, subtenon carboplatin injection or external beam radiotherapy. Alternatively, groups B, C and D tumors can be primarily treated with intraarterial chemotherapy. Intravitreal melphalan injection can be an alternative treatment for eyes with recurrent or persistent vitreous seeding. Group E eyes are often enucleated, however, depending on the fellow eye, systemic or intraarterial chemotherapy may be administered. Systemic chemotherapy is also mandatory if high-risk histopathological features are found after enucleation. (Turk J Ophthalmol 2014; 44: Supplement 22-8

  19. Masquerade syndrome:Retinoblastoma presenting as phacolytic glaucoma

    Institute of Scientific and Technical Information of China (English)

    Li-Jing Peng; Syed Shoeb Ahmad; Nor Haizura Bt Abd Rani; May May Choo; Shuaibah Abdul Ghani

    2016-01-01

    We aim to report an uncommon presentation of retinoblastoma and its management. A 4-year-old boy presented with left eye pain for 1 week with eye redness after being hit by a ball. On examination, the visual acuity in his right eye was 6/6 and left eye was no perception to light. The conjunctiva in his left eye was injected and cornea was edematous. There was pseudohypopyon inferiorly. Lens was opaque and there was no fundus view. Intraocular pressure was 50 mmHg. Patient was initially treated as phacolytic glaucoma with severe inflammation. B scan showed intralesional calcification with retinal detachment. CT orbit showed left intraocular mass with calcification and optic nerve involvement. Thus, our final diagnosis was left eye retinoblastoma. Patient was performed a enucleation with chemotherapy in left eye. One year after the treatment, there was no local and distant invasion of the tumor. Anterior inflammation is a rare form of masquerade syndrome associated with retinoblastoma.

  20. Estrategias de crecimiento empresarial aplicadas por hipermercados

    OpenAIRE

    Miraidy Leal Morantes; Yilian Cefalá Chirinos

    2003-01-01

    El presente estudio tiene como objetivo caracterizar las estrategias de crecimiento empresarial, representadas por alianzas y adquisiciones, que han sido aplicadas en el sector de distribución de bienes de consumo masivo, específicamente en los hipermercados ubicados en Maracaibo, Estado Zulia, como manera de responder al entorno competitivo y cambiante en el cual se desenvuelven. La investigación se desarrolló a través de un estudio de carácter descriptivo; para la recolección ...

  1. Inter-relacionamento de dados ambientais e de saúde: análise de risco à saúde aplicada ao abastecimento de água no Rio de Janeiro utilizando Sistemas de Informações Geográficas

    Directory of Open Access Journals (Sweden)

    Christovam Barcellos

    1998-07-01

    Full Text Available O inter-relacionamento de dados ambientais e sanitários, necessário na avaliação da exposição de grupos populacionais a fatores de risco, é dificultado pela defasagem no tempo e espaço destes conjuntos de dados. Neste trabalho utilizaram-se como bases de dados georreferenciadas: os setores censitários, que contêm informações sobre a forma com que são abastecidos os domicílios; a rede de abastecimento de água, seus principais mananciais e reservatórios; a qualidade da água, segundo programa de monitoramento. Mediante operações espaciais entre estas camadas, foram localizados e quantificados grupos populacionais submetidos a risco, de acordo com diferentes critérios. Grande parte dos riscos associados ao abastecimento de água encontram-se localizados na encosta norte do Maciço da Tijuca, e na Zona Oeste, onde a população procura formas alternativas de abastecimento. Em razão das diferentes origens, objetivos e estruturas dos dados, os Sistemas de Informações Geográficas (SIG podem ser utilizados como instrumento de organização, de validação destes dados e de verificação de possíveis inconsistências.

  2. Inter-relacionamento de dados ambientais e de saúde: análise de risco à saúde aplicada ao abastecimento de água no Rio de Janeiro utilizando Sistemas de Informações Geográficas

    Directory of Open Access Journals (Sweden)

    Barcellos Christovam

    1998-01-01

    Full Text Available O inter-relacionamento de dados ambientais e sanitários, necessário na avaliação da exposição de grupos populacionais a fatores de risco, é dificultado pela defasagem no tempo e espaço destes conjuntos de dados. Neste trabalho utilizaram-se como bases de dados georreferenciadas: os setores censitários, que contêm informações sobre a forma com que são abastecidos os domicílios; a rede de abastecimento de água, seus principais mananciais e reservatórios; a qualidade da água, segundo programa de monitoramento. Mediante operações espaciais entre estas camadas, foram localizados e quantificados grupos populacionais submetidos a risco, de acordo com diferentes critérios. Grande parte dos riscos associados ao abastecimento de água encontram-se localizados na encosta norte do Maciço da Tijuca, e na Zona Oeste, onde a população procura formas alternativas de abastecimento. Em razão das diferentes origens, objetivos e estruturas dos dados, os Sistemas de Informações Geográficas (SIG podem ser utilizados como instrumento de organização, de validação destes dados e de verificação de possíveis inconsistências.

  3. Caos, complexidade e Lingüística Aplicada: diálogos transdisciplinares

    Directory of Open Access Journals (Sweden)

    Antônio Carlos Soares Martins

    2007-01-01

    Full Text Available Neste texto, apresentamos um panorama de estudos na área da Lingüística Aplicada que discutem questões relativas ao processo de desenvolvimento de segunda língua, bem como aspectos relacionados aos ambientes interacionais de aprendizagem na perspectiva das teorias do Caos e Complexidade. Para isso, discutimos as principais noções da perspectiva da complexidade, retomando alguns aspectos da física clássica e contemporânea.In this paper, we present a panorama of studies in the area of Applied Linguistics that discuss questions regarding second language development as well as aspects related to interactional learning environments in the light of Chaos and Complexity theories. To accomplish this, we discuss the main notions of complexity through the rethinking of some aspects of classic and contemporary physics.

  4. Next generation sequencing in sporadic retinoblastoma patients reveals somatic mosaicism.

    Science.gov (United States)

    Amitrano, Sara; Marozza, Annabella; Somma, Serena; Imperatore, Valentina; Hadjistilianou, Theodora; De Francesco, Sonia; Toti, Paolo; Galimberti, Daniela; Meloni, Ilaria; Cetta, Francesco; Piu, Pietro; Di Marco, Chiara; Dosa, Laura; Lo Rizzo, Caterina; Carignani, Giulia; Mencarelli, Maria Antonietta; Mari, Francesca; Renieri, Alessandra; Ariani, Francesca

    2015-11-01

    In about 50% of sporadic cases of retinoblastoma, no constitutive RB1 mutations are detected by conventional methods. However, recent research suggests that, at least in some of these cases, there is somatic mosaicism with respect to RB1 normal and mutant alleles. The increased availability of next generation sequencing improves our ability to detect the exact percentage of patients with mosaicism. Using this technology, we re-tested a series of 40 patients with sporadic retinoblastoma: 10 of them had been previously classified as constitutional heterozygotes, whereas in 30 no RB1 mutations had been found in lymphocytes. In 3 of these 30 patients, we have now identified low-level mosaic variants, varying in frequency between 8 and 24%. In 7 out of the 10 cases previously classified as heterozygous from testing blood cells, we were able to test additional tissues (ocular tissues, urine and/or oral mucosa): in three of them, next generation sequencing has revealed mosaicism. Present results thus confirm that a significant fraction (6/40; 15%) of sporadic retinoblastoma cases are due to postzygotic events and that deep sequencing is an efficient method to unambiguously distinguish mosaics. Re-testing of retinoblastoma patients through next generation sequencing can thus provide new information that may have important implications with respect to genetic counseling and family care.

  5. Retinoblastoma in Adolescence: Report of an Unusual Presentation

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Khalili

    2010-04-01

    Full Text Available Herein, we report the clinical, pathologic and photographic findings of a 16year-old girl who presented with gradual enlargement of a vascularized, hemorrhagicperipapillary mass that eventually led to vitreous hemorrhage and rubeosis iridis.Histopathological findings after enucleation were positive for retinoblastoma. Thepatient did not have any metastasis during her 20 months of follow up.

  6. Photoacoustic imaging features of intraocular tumors: Retinoblastoma and uveal melanoma

    Science.gov (United States)

    Xu, Guan; Xue, Yafang; Özkurt, Zeynep Gürsel; Slimani, Naziha; Hu, Zizhong; Wang, Xueding; Xia, Kewen; Ma, Teng; Zhou, Qifa; Demirci, Hakan

    2017-01-01

    The purpose of this study is to examine the capability of photoacoustic (PA) imaging (PAI) in assessing the unique molecular and architectural features in ocular tumors. A real-time PA and ultrasonography (US) parallel imaging system based on a research US platform was developed to examine retinoblastoma in mice in vivo and human retinoblastoma and uveal melanoma ex vivo. PA signals were generated by optical illumination at 720, 750, 800, 850, 900 and 950 nm delivered through a fiber optical bundle. The optical absorption spectra of the tumors were derived from the PA images. The optical absorption spectrum of each tumor was quantified by fitting to a polynomial model. The microscopic architectures of the tumors were quantified by frequency domain analysis of the PA signals. Both the optical spectral and architectural features agree with the histological findings of the tumors. The mouse and human retinoblastoma showed comparable total optical absorption spectra at a correlation of 0.95 (p<0.005). The quantitative PAI features of human retinoblastoma and uveal melanoma have shown statistically significant difference in two tailed t-tests (p<0.05). Fully compatible with the concurrent procedures, PAI could be a potential tool complementary to other diagnostic modalities for characterizing intraocular tumors. PMID:28231293

  7. Inter-relacionamento de dados ambientais e de saúde: análise de risco à saúde aplicada ao abastecimento de água no Rio de Janeiro utilizando Sistemas de Informações Geográficas Linkage of environmental and health data: health risk analysis of the Rio de Janeiro water supply using Geographical Information Systems

    Directory of Open Access Journals (Sweden)

    Christovam Barcellos

    1998-07-01

    Full Text Available O inter-relacionamento de dados ambientais e sanitários, necessário na avaliação da exposição de grupos populacionais a fatores de risco, é dificultado pela defasagem no tempo e espaço destes conjuntos de dados. Neste trabalho utilizaram-se como bases de dados georreferenciadas: os setores censitários, que contêm informações sobre a forma com que são abastecidos os domicílios; a rede de abastecimento de água, seus principais mananciais e reservatórios; a qualidade da água, segundo programa de monitoramento. Mediante operações espaciais entre estas camadas, foram localizados e quantificados grupos populacionais submetidos a risco, de acordo com diferentes critérios. Grande parte dos riscos associados ao abastecimento de água encontram-se localizados na encosta norte do Maciço da Tijuca, e na Zona Oeste, onde a população procura formas alternativas de abastecimento. Em razão das diferentes origens, objetivos e estruturas dos dados, os Sistemas de Informações Geográficas (SIG podem ser utilizados como instrumento de organização, de validação destes dados e de verificação de possíveis inconsistências.Exposure assessment of population groups is based on linkage of environmental and health data. This relationship can be hard to establish due to spatial and temporal lags in data sets. GIS can be used as a basis for organizing health-related and environmental data sets. We examined potential health risk in the Rio de Janeiro city water supply based on the overlay of information layers containing data on the presence and quality of water supply services. We used census tracts as the primary georeferenced data, since they contain information on how households are supplied, water supply pipes, sources, and reservoirs, and water quality according to the monitoring program. Population groups exposed to risks were located and quantified using spatial operations among these layers and adopting different risk criteria. The

  8. Can preoperative MR imaging predict optic nerve invasion of retinoblastoma?

    Energy Technology Data Exchange (ETDEWEB)

    Song, Kyoung Doo, E-mail: kdsong0308@gmail.com [Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50, Ilwon-Dong, Kangnam-Ku, Seoul 135-710 (Korea, Republic of); Eo, Hong, E-mail: rtombow@gmail.com [Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50, Ilwon-Dong, Kangnam-Ku, Seoul 135-710 (Korea, Republic of); Kim, Ji Hye, E-mail: jhkate.kim@samsung.com [Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50, Ilwon-Dong, Kangnam-Ku, Seoul 135-710 (Korea, Republic of); Yoo, So-Young, E-mail: sy1131.yoo@samsung.com [Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50, Ilwon-Dong, Kangnam-Ku, Seoul 135-710 (Korea, Republic of); Jeon, Tae Yeon, E-mail: hathor97.jeon@samsung.com [Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50, Ilwon-Dong, Kangnam-Ku, Seoul 135-710 (Korea, Republic of)

    2012-12-15

    Purpose: To evaluate the accuracy of pre-operative MRI for the detection of optic nerve invasion in retinoblastoma. Materials and methods: Institutional review board approval and informed consent were waived for this retrospective study. A total of 41 patients were included. Inclusion criteria were histologically proven retinoblastoma, availability of diagnostic-quality preoperative MR images acquired during the 4 weeks before surgery, unilateral retinoblastoma, and normal-sized optic nerve. Two radiologists retrospectively reviewed the MR images independently. Five imaging findings (diffuse mild optic nerve enhancement, focal strong optic nerve enhancement, optic sheath enhancement, tumor location, and tumor size) were evaluated against optic nerve invasion of retinoblastoma. The predictive performance of all MR imaging findings for optic nerve invasion was also evaluated by the receiver operating characteristic curve analysis. Results: Optic nerve invasion was histopathologically confirmed in 24% of study population (10/41). The differences in diffuse mild enhancement, focal strong enhancement, optic sheath enhancement, and tumor location between patients with optic nerve invasion and patients without optic nerve invasion were not significant. Tumor sizes were 16.1 mm (SD: 2.2 mm) and 14.9 mm (SD: 3.6 mm) in patients with and without optic nerve involvement, respectively (P = 0.444). P-Values from binary logistic regression indicated that all five imaging findings were not significant predictors of tumor invasion of optic nerve. The AUC values of all MR imaging findings for the prediction of optic nerve invasion were 0.689 (95% confidence interval: 0.499–0.879) and 0.653 (95% confidence interval: 0.445–0.861) for observer 1 and observer 2, respectively. Conclusion: Findings of MRI in patients with normal-sized optic nerves have limited usefulness in preoperatively predicting the presence of optic nerve invasion in retinoblastoma.

  9. Proton Radiation Therapy for the Treatment of Retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Mouw, Kent W. [Harvard Radiation Oncology Program, Boston, Massachusetts (United States); Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts (United States); Sethi, Roshan V.; Yeap, Beow Y.; MacDonald, Shannon M.; Chen, Yen-Lin E.; Tarbell, Nancy J.; Yock, Torunn I.; Munzenrider, John E.; Adams, Judith [Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts (United States); Grabowski, Eric [Department of Pediatrics, Massachusetts General Hospital, Boston, Massachusetts (United States); Mukai, Shizuo [Retina Service, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts (United States); Shih, Helen A., E-mail: hshih@partners.org [Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts (United States)

    2014-11-15

    Purpose: To investigate long-term disease and toxicity outcomes for pediatric retinoblastoma patients treated with proton radiation therapy (PRT). Methods and Materials: This is a retrospective analysis of 49 retinoblastoma patients (60 eyes) treated with PRT between 1986 and 2012. Results: The majority (84%) of patients had bilateral disease, and nearly half (45%) had received prior chemotherapy. At a median follow-up of 8 years (range, 1-24 years), no patients died of retinoblastoma or developed metastatic disease. The post-PRT enucleation rate was low (18%), especially in patients with early-stage disease (11% for patients with International Classification for Intraocular Retinoblastoma [ICIR] stage A-B disease vs 23% for patients with ICIR stage C-D disease). Post-PRT ophthalmologic follow-up was available for 61% of the preserved eyes (30 of 49): 14 of 30 eyes (47%) had 20/40 visual acuity or better, 7 of 30 (23%) had moderate visual acuity (20/40-20/600), and 9 of 30 (30%) had little or no useful vision (worse than 20/600). Twelve of 60 treated eyes (20%) experienced a post-PRT event requiring intervention, with cataracts the most common (4 eyes). No patients developed an in-field second malignancy. Conclusions: Long-term follow-up of retinoblastoma patients treated with PRT demonstrates that PRT can achieve high local control rates, even in advanced cases, and many patients retain useful vision in the treated eye. Treatment-related ocular side effects were uncommon, and no radiation-associated malignancies were observed.

  10. Análise de risco aplicada ao manejo precaucionário das pescarias artesanais na região do reservatório da UHE-Tucuruí (Pará, Brasil Risk analysis applied to the precautionary management of artisanal fisheries in the region of Tucuruí reservoir (Pará, Brazil

    Directory of Open Access Journals (Sweden)

    Serguei Aily Franco de Camargo

    2004-09-01

    Full Text Available As pescarias no reservatório da UHE-Tucuruí no rio Tocantins, Pará, envolvem cerca de 6.000 pescadores e movimentam cerca de R$ 4,2 milhões/ano. A atividade se concentra em três espécies principais: tucunaré Cichla monoculus (capturado com anzol, pescada Plagioscion squamosissimus (capturado com rede e/ou anzol e mapará Hypophthalmus marginatus (capturado com rede. Com o objetivo de caracterizar os pescadores e as pescarias do reservatório, criar cenários de aumento do esforço pesqueiro e prever os momentos de conflito pela escassez de recursos, foram levantadas informações da literatura e realizadas duas campanhas de coleta de dados nos anos de 1999 e 2000, envolvendo entrevistas com líderes comunitários e pescadores. As seguintes variáveis foram consideradas: desembarque por espécie-alvo (de acordo com os registros fornecidos pelas colônias de pescadores, artes de pesca, estratégias dos pescadores, conflitos e formas de apropriação do espaço e rendimentos da atividade. Estas variáveis foram inseridas em um modelo dinâmico, simulado no software Vensim PLE para um período de 10 anos a partir de 1999. Os resultados indicam que a pesca de anzol é a estratégia mais rentável, e que possíveis momentos de conflito devido à escassez de recursos podem acontecer em curto prazo (2005. A metodologia utilizada para as simulações e análises de risco também se revelou adequada à realidade local e ao conjunto de dados disponíveis.The fisheries in the reservoir of UHE-Tucuruí employ about 6.000 fishermen generating approximately R$ 4.2 million/year. There are three main target-species: Tucunaré Cichla monoculus (caught with hook and line, Pescada Plagioscion squamosissimus (caught with gill net and/or hook and line and Mapará Hypophthalmus marginatus (caught with gill net. Published information was reviewed and data collected during two field trips in 1999 and 2000 that included interviews with community leaders and

  11. Neutrongraphy technique applied to the narcotics and terrorism enforcement; Neutrongrafia aplicada no combate ao narcotrafico e ao terrorismo

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Ademir X. da; Hacidume, Leo R.; Crispim, Verginia R. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia. Programa de Engenharia Nuclear

    1999-03-01

    Among the several methods of non-destructive essays that may be used for the detection of both drugs and explosives, the ones that utilize nuclear techniques have demonstrated to possess essential qualities for an efficient detection system. These techniques allow the inspection of a large quantity of samples fast, sensibly, specifically and with automatic decision, for they utilize radiation of great power of penetration. This work aims to show the neutron radiography and computed tomography potentiality for the detection of the drugs and explosives even when they are concealed by heavy materials. In the radiographic essays with thermal neutrons, samples of powder cocaine and explosives were inspected, concealed by several materials or not. The samples were irradiated during 30 minutes in the J-9 channel of the Argonauta research reactor of the IEN/CNEN in a neutron flux of 2:5 10{sup 5} n/cm{sup 2}.s. We used two sheets of gadolinium converter with a thickness of 25 {mu}m each one and a Kodak Industrex A5 photographic plaque. A comparative analysis among the tomographic images experimental and simulated obtained by X-ray, fast and thermal neutron is presented. The thermal neutron tomography demonstrate to be the best. (author)

  12. MicroRNAs-449a and -449b exhibit tumor suppressive effects in retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Martin, Alissa [Division of Hematology, Oncology, and Stem Cell Transplantation, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611 (United States); Jones, Aunica [Cancer Biology and Epigenomics Program, Ann and Robert H. Lurie Children’s Hospital of Chicago Research Center, Chicago, IL 60611 (United States); Bryar, Paul J. [Departments of Ophthalmology and Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611 (United States); Mets, Marilyn [Division of Ophthalmology, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611 (United States); Department of Ophthalmology, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 (United States); Weinstein, Joanna [Department of Pediatrics, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 (United States); Division of Hematology, Oncology, and Stem Cell Transplantation, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611 (United States); Zhang, Gang [Biostatistics Research Core, Ann and Robert H. Lurie Children’s Hospital of Chicago Research Center, Chicago, IL 60611 (United States); Laurie, Nikia A., E-mail: n-laurie@northwestern.edu [Cancer Biology and Epigenomics Program, Ann and Robert H. Lurie Children’s Hospital of Chicago Research Center, Chicago, IL 60611 (United States); Department of Pediatrics, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 (United States)

    2013-11-01

    Highlights: •We validate miR-449a/b expression in primary human retinoblastomas and cell lines. •Exogenous miRs-449a/b inhibited proliferation in retinoblastoma cell lines. •Exogenous miRs-449a/b increased apoptosis in retinoblastoma cell lines. •miRs-449a/b could serve as viable therapeutic targets for retinoblastoma treatment. -- Abstract: Retinoblastoma is the most common pediatric cancer of the eye. Currently, the chemotherapeutic treatments for retinoblastoma are broad-based drugs such as vincristine, carboplatin, or etoposide. However, therapies targeted directly to aberrant signaling pathways may provide more effective therapy for this disease. The purpose of our study is to illustrate the relationship between the expressions of miRs-449a and -449b to retinoblastoma proliferation and apoptosis. We are the first to confirm an inhibitory effect of miR-449a and -449b in retinoblastoma by demonstrating significantly impaired proliferation and increased apoptosis of tumor cells when these miRNAs are overexpressed. This study suggests that these miRNAs could serve as viable therapeutic targets for retinoblastoma treatment.

  13. Risk of retinoblastoma is associated with a maternal polymorphism in dihydrofolatereductase (DHFR) and prenatal folic acid intake

    Science.gov (United States)

    The incidence of unilateral retinoblastoma varies globally, suggesting possible environmental contributors to disease incidence. Maternal intake of naturally occurring folate from vegetables during pregnancy is associated inversely with the risk of retinoblastoma in offspring. The authors used a ca...

  14. Genetic perspective of retinoblastoma: From present to future

    OpenAIRE

    2016-01-01

    Retinoblastoma (RB) is the most common malignant intraocular tumor in children. In the last decade, basic research has led to a better understanding of events after two hits in RB susceptibility gene (RB1), molecular mechanism of tumor growth, the cell of origin of RB, etc. This would pave way to identify biomarkers and molecular targeted therapy for better treatment option in the future. Furthermore, improvement in molecular techniques has led to enhanced diagnostic methods for early diagnos...

  15. Efficient photodynamic therapy on human retinoblastoma cell lines.

    Directory of Open Access Journals (Sweden)

    Jan Walther

    Full Text Available Photodynamic therapy (PDT has shown to be a promising technique to treat various forms of malignant neoplasia. The photodynamic eradication of the tumor cells is achieved by applying a photosensitizer either locally or systemically and following local activation through irradiation of the tumor mass with light of a specific wavelength after a certain time of incubation. Due to preferential accumulation of the photosensitizer in tumor cells, this procedure allows a selective inactivation of the malignant tumor while sparing the surrounding tissue to the greatest extent. These features and requirements make the PDT an attractive therapeutic option for the treatment of retinoblastoma, especially when surgical enucleation is a curative option. This extreme solution is still in use in case of tumours that are resistant to conventional chemotherapy or handled too late due to poor access to medical care in less advanced country. In this study we initially conducted in-vitro investigations of the new cationic water-soluble photo sensitizer tetrahydroporphyrin-tetratosylat (THPTS regarding its photodynamic effect on human Rb-1 and Y79 retinoblastoma cells. We were able to show, that neither the incubation with THPTS without following illumination, nor the sole illumination showed a considerable effect on the proliferation of the retinoblastoma cells, whereas the incubation with THPTS combined with following illumination led to a maximal cytotoxic effect on the tumor cells. Moreover the phototoxicity was lower in normal primary cells from retinal pigmented epithelium demonstrating a higher phototoxic effect of THPTS in cancer cells than in this normal retinal cell type. The results at hand form an encouraging foundation for further in-vivo studies on the therapeutic potential of this promising photosensitizer for the eyeball and vision preserving as well as potentially curative therapy of retinoblastoma.

  16. Efficient Photodynamic Therapy on Human Retinoblastoma Cell Lines

    Science.gov (United States)

    Walther, Jan; Schastak, Stanislas; Dukic-Stefanovic, Sladjana; Wiedemann, Peter; Neuhaus, Jochen; Claudepierre, Thomas

    2014-01-01

    Photodynamic therapy (PDT) has shown to be a promising technique to treat various forms of malignant neoplasia. The photodynamic eradication of the tumor cells is achieved by applying a photosensitizer either locally or systemically and following local activation through irradiation of the tumor mass with light of a specific wavelength after a certain time of incubation. Due to preferential accumulation of the photosensitizer in tumor cells, this procedure allows a selective inactivation of the malignant tumor while sparing the surrounding tissue to the greatest extent. These features and requirements make the PDT an attractive therapeutic option for the treatment of retinoblastoma, especially when surgical enucleation is a curative option. This extreme solution is still in use in case of tumours that are resistant to conventional chemotherapy or handled too late due to poor access to medical care in less advanced country. In this study we initially conducted in-vitro investigations of the new cationic water-soluble photo sensitizer tetrahydroporphyrin-tetratosylat (THPTS) regarding its photodynamic effect on human Rb-1 and Y79 retinoblastoma cells. We were able to show, that neither the incubation with THPTS without following illumination, nor the sole illumination showed a considerable effect on the proliferation of the retinoblastoma cells, whereas the incubation with THPTS combined with following illumination led to a maximal cytotoxic effect on the tumor cells. Moreover the phototoxicity was lower in normal primary cells from retinal pigmented epithelium demonstrating a higher phototoxic effect of THPTS in cancer cells than in this normal retinal cell type. The results at hand form an encouraging foundation for further in-vivo studies on the therapeutic potential of this promising photosensitizer for the eyeball and vision preserving as well as potentially curative therapy of retinoblastoma. PMID:24498108

  17. {sup 106}Ruthenium Plaque Therapy (RPT) for Retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Murakami, Naoya, E-mail: namuraka@ncc.go.jp [Department of Radiation Oncology, National Cancer Center Hospital, Tokyo (Japan); Suzuki, Shigenobu [Department of Ophthalmic Oncology, National Cancer Center Hospital, Tokyo (Japan); Ito, Yoshinori [Department of Radiation Oncology, National Cancer Center Hospital, Tokyo (Japan); Yoshimura, Ryoichi [Department of Diagnostic Radiology and Oncology, Head and Neck Reconstruction Division, Graduate School, Tokyo Medical and Dental University, Tokyo (Japan); Inaba, Koji; Kuroda, Yuki; Morota, Madoka; Mayahara, Hiroshi; Sakudo, Mototake; Wakita, Akihisa; Okamoto, Hiroyuki; Sumi, Minako; Kagami, Yoshikazu [Department of Radiation Oncology, National Cancer Center Hospital, Tokyo (Japan); Nakagawa, Keiichi; Ohtomo, Kuni [Department of Radiology, University of Tokyo Hospital, Tokyo (Japan); Itami, Jun [Department of Radiation Oncology, National Cancer Center Hospital, Tokyo (Japan)

    2012-09-01

    Purpose: To evaluate the effectiveness of episcleral {sup 106}ruthenium plaque therapy (RPT) in the management of retinoblastoma. Methods and Materials: One hundred one RPTs were retrospectively analyzed that were performed in 90 eyes of 85 patients with retinoblastoma at National Cancer Center Hospital between 1998 and 2008. Each RPT had a corresponding tumor and 101 tumors were considered in the analysis of local control. Median follow-up length was 72.8 months. Median patient age at the RPT was 28 months. Median prescribed doses at reference depth and outer surface of the sclera were 47.4 Gy and 162.3 Gy, respectively. Results: Local control rate (LCR) and ocular retention rate (ORR) at 2 years were 33.7% and 58.7%, respectively. Unilateral disease, International Classification of Retinoblastoma group C or more advanced at the first presentation or at the time of RPT, vitreous and/or subretinal seeding, tumor size greater than 5 disc diameter (DD), reference depth greater than 5 mm, dose rate at reference depth lower than 0.7 Gy/hour, dose at the reference depth lower than 35 Gy, and (biologically effective dose with an {alpha}/{beta} ratio of 10 Gy) at the reference depth lower than 40 Gy{sub 10} were associated with unfavorable LCR. Two patients died of metastatic disease. Radiation complications included retinal detachment in 12 eyes (13.3%), proliferative retinopathy in 6 (6.7%), rubeosis iris in 2 (2.2%), and posterior subcapsular cataract in 23 (25.6%). Conclusion: RPT is an effective eye-preserving treatment for retinoblastoma.

  18. Estrategias de crecimiento empresarial aplicadas por hipermercados

    Directory of Open Access Journals (Sweden)

    Miraidy Leal Morantes

    2003-01-01

    Full Text Available El presente estudio tiene como objetivo caracterizar las estrategias de crecimiento empresarial, representadas por alianzas y adquisiciones, que han sido aplicadas en el sector de distribución de bienes de consumo masivo, específicamente en los hipermercados ubicados en Maracaibo, Estado Zulia, como manera de responder al entorno competitivo y cambiante en el cual se desenvuelven. La investigación se desarrolló a través de un estudio de carácter descriptivo; para la recolección de datos se aplicó un cuestionario estructurado, bajo la modalidad de entrevista, a gerentes generales y financieros. Los resultados evidencian que las estrategias de crecimiento empresarial utilizadas son la apertura de nuevos establecimientos comerciales, adquisición de empresas y participación accionaria en grandes cadenas, las cuales propician la concentración del mercado de distribución comercial, afectando a algunas empresas venezolanas del sector cuya oferta no puede competir; asimismo, los proveedores se enfrentan a cadenas con gran poder de negociación.

  19. ANÁLISE ONTOLOLÓGICA APLICADA AO DESENVOLVIMENTO DE FRAMES

    Directory of Open Access Journals (Sweden)

    Maria Margarida Martins Salomão

    2012-01-01

    Full Text Available Semântica de Frames é uma teoria que busca estabelecer a semântica de um item lexical pela evocação da cena conceitual a ele associada. A FrameNet é uma base léxicosemântica fundamentada na Semântica de Frames, constituída por um conjunto de frames relacionados por ligações semânticas. A metodologia de descoberta e incorporação de frames à base obedece a critérios semiformais e incorporam alguns critérios ontológicos. Neste artigo, buscamos o aprofundamento dessa análise ontológica de forma a permitir maior formalização do processo. Para isso, analisamos sentenças do Corpus do Português contendo o lexema viagem e alinhamos os elementos de frames com os conceitos pertencentes a ontologias de topo. Em seguida, detectamos as sentenças que incorporavam o sentido prototípico apresentado na FrameNet. Além disso, identificamos o uso metafórico do lexema e seu uso com o sentido de transporte a partir da distinção do tipo ontológico do agente. Assim, a partir da análise ontológica foi possível encontrar um novo frame ainda não definido na base FrameNet. Essas evidências sugerem possíveis benefícios da metodologia proposta, podendo facilitar, futuramente, uma formalização na ligação de frames distintos motivados pela polissemia de um lexema. Acreditamos, dessa forma, favorecer a inferência textual automática e o uso da rede por pessoas.

  20. Projeto Fidelização de clientes aplicada ao restaurante Tribeca

    OpenAIRE

    Monteiro, Ana Luísa Ferreira

    2012-01-01

    Dissertação de Mestrado em Marketing e Promoção Turística apresentada à ESTM - Escola Superior de Turismo e Tecnologia do Mar do Instituto Politécnico de Leiria Atualmente, para fazer face às exigências, necessidades e expetativas dos clientes muitas empresas recorrem à utilização de diversas ações, no sentido de manter os seus clientes e conquistar novos, mas a concorrência entre empresas é uma constante e nenhuma tem a capacidade de saber qual a melhor forma de conseguir alcançar os seus...

  1. Metapsicologia aplicada ao direito inconsciente freudiano: uma introdução

    OpenAIRE

    Ilder Miranda Costa

    2010-01-01

    Pesquisas sobre relações do Direito com outras áreas do saber deverão, cada vez mais, ocupar as preocupações do mundo jurídico acadêmico. O pressentimento, contido na asserção anterior, possui um único motivo: em 29 de setembro de 2004, o Conselho Nacional de Educação do Ministério da Educação, conforme Resolução CNE/CSE nº 9, determinou que: Art. 5º O curso de graduação em Direito deverá contemplar, em seu Projeto Pedagógico e em sua Organização Curricular, conteúdos e atividades que atendam...

  2. Espectroscopia Raman aplicada ao estudo da resposta bioquímica de tecidos a compostos lamelares

    OpenAIRE

    Otávio Mendes Gil

    2015-01-01

    Nesta Dissertação de Mestrado foram estudadas as respostas inflamatórias e de reparo de tecido muscular de ratos a implantes de hidróxidos duplos lamelares (HDL) contendo íon cloreto e sulindaco (fármaco anti-inflamatório não-esteroidal - FAINE) em seu espaço interlamelar. As técnicas empregadas nesta investigação foram as espectroscopias Raman e de absorção no infravermelho (FTIR), a difratometria de raios X (XRD) e a análise histomorfológica dos tecidos corados pelas técnicas de Hematoxilin...

  3. Nanotecnologia aplicada ao sistema cardiovascular: efeito do fulerol no estresse oxidativo

    OpenAIRE

    Marina de Souza Ladeira

    2013-01-01

    O estresse oxidativo decorrente do aumento da concentração de espécies reativas de oxigênio (ERO) está associado à progressão de diversas disfunções cardiovasculares. No coração, o estresse oxidativo pode afetar a estrutura e função do miocárdio, estimulando o processo de hipertrofia e apoptose dos miócitos. Além do efeito no coração, o estresse oxidativo está associado à disfunção endotelial, podendo promover alterações no tônus vascular. Diante disso, o tratamento com agentes antioxidant...

  4. Análise ontolológica aplicada ao desenvolvimento de Frames

    Directory of Open Access Journals (Sweden)

    Alexandra Moreira

    2012-12-01

    Full Text Available Semântica de Frames é uma teoria que busca estabelecer a semântica de um item lexical pela evocação da cena conceitual a ele associada. A FrameNet é uma base léxico-semântica fundamentada na Semântica de Frames, constituída por um conjunto de frames relacionados por ligações semânticas. A metodologia de descoberta e incorporação de frames à base obedece a critérios semiformais e incorporam alguns critérios ontológicos. Neste artigo, buscamos o aprofundamento dessa análise ontológica de forma a permitir maior formalização do processo. Para isso, analisamos sentenças do Corpus do Português contendo o lexema viagem e alinhamos os elementos de frames com os conceitos pertencentes a ontologias de topo. Em seguida, detectamos as sentenças que incorporavam o sentido prototípico apresentado na FrameNet. Além disso, identificamos o uso metafórico do lexema e seu uso com o sentido de transporte a partir da distinção do tipo ontológico do agente. Assim, a partir da análise ontológica foi possível encontrar um novo frame ainda não definido na base FrameNet. Essas evidências sugerem possíveis benefícios da metodologia proposta, podendo facilitar, futuramente, uma formalização na ligação de frames distintos motivados pela polissemia de um lexema. Acreditamos, dessa forma, favorecer a inferência textual automática e o uso da rede por pessoas.

  5. A METODOLOGIA DE CALLISTA ROY APLICADA EM CLIENTES SUBMETIDOS À INTERVENÇAO HEMODINÂMICA

    Directory of Open Access Journals (Sweden)

    Ivete Maroso Krauzer

    2011-01-01

    Full Text Available RESUMO: Trata-se de uma pesquisa qualitativa, do tipo exploratório descritiva, que identificou o perfil de saúde dos clientes em tratamento hemodinâmico e desenvolveu o Processo de Enfermagem (PE, fundamentado em Callista Roy. Realizou-se com 52 clientes, no Centro de Hemodinâmica de um hospital privado, de fevereiro a maio de 2010. Utilizouse um questionário semiestruturado para coleta de dados. A maioria tinha mais de 60 anos, aposentados, casados, moradores de Chapecó, com ensino fundamental. Os fatores de risco principais foram: idade acima de 60 anos, a Hipertensão Arterial Sistêmica (HAS,obesidade e vida sedentária. Os principais diagnósticos de enfermagem foram: ansiedade relacionada à morte (100%; risco de dor aguda (85% e integridade da pele prejudicada (75%. Os resultados obtidos podem contribuir para a enfermagem embasar o cuidado no PE, identificar os fatores de risco, atuar na prevenção e num serviço de alta complexidade que exige conhecimentos especializados.

  6. Cariometria aplicada a avaliaçao da anaplasia em tumores ependimários

    OpenAIRE

    Silva, Antonio de Padua Gomes da

    1987-01-01

    Resumo: Foram realizadas 1.500 medições de volumes de núcleos, através de cariometria com ocular micrométrica simples, para comparação entre células ependimárias normais e neoplásicas, determinando-se, matematicamente, o aumento de volume que existe quando a célula normal se dediferencia em célula neoplásica.

  7. Peso y talla en niños con retinoblastoma

    Directory of Open Access Journals (Sweden)

    Cecilia Ridaura-Sanz

    2015-03-01

    Full Text Available Introducción: la talla baja se ha descrito como característica clínica de niños con retinoblastoma. Esta particularidad puede estar relacionada directamente con la enfermedad de base o con factores externos.  Objetivo: el propósito de esta investigación fue conocer la frecuencia de talla y peso bajos en niños mexicanos con retinoblastoma y correlacionar los valores antropométricos con variables de la enfermedad y ambientales. Materiales y métodos: se analizaron expedientes clínicos de 346 pacientes con retinoblastoma. Se obtuvieron los datos de peso y talla referidos al ingreso; antes del tratamiento. Se comparó el valor de Z con las referencias de la distribución normal de la población mexicana. La asociación de las medidas antropométricas con las variables clínicas, sociales y genéticas se estableció con la prueba de c2. Resultados: la talla y el peso promedio de los niños con retinoblastoma fueron significativamente más bajos que los de la población general (p < 0.001 y p = 0.013, respectivamente. La talla y peso bajos fueron más frecuentes en niños con enfermedad avanzada, provenientes de medio rural y de bajo nivel socioeconómico. Conclusiones: los déficits en peso y talla en niños con retinoblastoma se deben a factores relacionados con enfermedad avanzada al momento del diagnóstico, lo cual a su vez es consecuencia de la situación de marginación de la población rural mexicana. Dado que la mayoría de las variables sociales y ambientales están muy relacionadas y que no podemos descartar posibles factores genéticos, las conclusiones de este estudio deben verificarse analizando las medidas de los padres y hermanos y establecer grupos comparativos para controlar las variables confusas.

  8. Incidence of retinoblastoma in Dutch children conceived by IVF: an expanded study.

    NARCIS (Netherlands)

    Marees, T.; Dommering, C.J.; Imhof, S.M.; Kors, W.A.; Ringens, P.J.; Leeuwen, F.E. van; Moll, A.C.

    2009-01-01

    BACKGROUND: In 2003, we reported an increased risk of retinoblastoma in children conceived by IVF between 1995 and 2002. However, population-based studies among children conceived by IVF did not find an elevated risk of retinoblastoma. METHODS: From nationwide estimates of numbers of live births con

  9. Naturally death-resistant precursor cells revealed as the origin of retinoblastoma

    DEFF Research Database (Denmark)

    Trinh, Emmanuelle; Lazzerini Denchi, Eros; Helin, Kristian

    2004-01-01

    The molecular mechanisms and the cell-of-origin leading to retinoblastoma are not well defined. In this issue of Cancer Cell, Bremner and colleagues describe the first inheritable model of retinoblastoma, revealing that loss of the pocket proteins pRb and p107 deregulates cell cycle exit in retin...

  10. Ensaios sobre desenvolvimento econômico: uma abordagem neo-schumpeteriana aplicada à economia brasileira

    OpenAIRE

    Daniel de Souza Coelho

    2010-01-01

    A análise de contabilidade do crescimento aplicada ao Brasil, no período de 1950 a 2007, indica que o crescimento brasileiro não é explicado de forma satisfatória pelas teorias que tratam o progresso técnico como exógeno. Usando o modelo neo-schumpeteriano, explorou-se o papel da competição de mercado no crescimento da produtividade e do esforço inovativo das firmas usando as bases de dados da PAEP/SEADE e da PIA/IBGE. A importância do tamanho da firma nessas análises estimulou a investigação...

  11. The Drug Sensitivity of Cyclosporine A Combined with Antineoplastic Drugs in Retinoblastoma in Vitro

    Institute of Scientific and Technical Information of China (English)

    Wanli Liu; Zhongyao Wu

    2005-01-01

    Purpose: To study cyclosporine A inhibition on the fresh retinoblastoma cells in vitro and increasing the drug sensitivity after combined with different antineoplastic drugs.Methods: To study the growth curve of cyclosporine A on 27 samples of primary retinoblastoma cells by MTT assay and to study the change of the drug sensitivity by cyclosporine A combined with seven antineoplastic drugs.Results: The average IC50 of cyclosporine A on the 27 retinoblastoma cells is 67.81μg/ml and the average inhibitive rate of these samples is 26.1% when cyclosporine A is in the concentration of 2μg/ml. The inhibitive rates all got improved after the seven antineoplastic drugs combined with cyclosporine A and the increasing average inhibitive rate is more than 5.Conclusion: Cyclosporine A can inhibit retinoblastoma cells in vitro and its inhibitive effect is dose dependent. Moreover it can enhance the inhibition of multiple antineoplastic drugs on retinoblastoma cells.

  12. Molecular-genetic analysis of two cases with retinoblastoma: benefits for disease management

    Indian Academy of Sciences (India)

    Govindasamy Kumaramanickavel; Biju Joseph; Komaravelly Narayana; Sribhargava Natesh; Gandra Mamatha; Mahesh P. Shanmugam; Anuradha Elamparathi; Jyotirmay Biswas

    2003-04-01

    Effective counselling and management of retinoblastoma families using genetic information is presently practised in many parts of the world. We studied histopathological, chromosomal and molecular-genetic data of two retinoblastoma patients from India. The two patients, one with bilateral and the other with unilateral retinoblastoma, underwent complete ophthalmic examination, cytogenetic study, retinoblastoma gene (RB1) mutational analysis and RB1 promoter region methylation screening. In the bilateral retinoblastoma patient deletion of chromosome region 13q14 in peripheral blood lymphocytes and a hemizygous novel 8-bp deletion in exon 4 of RB1 in tumour sample were observed. In the unilaterally affected patient CGA to TGA transition protein truncation mutations were observed in exons 8 and 14 of RB1.

  13. Uma breve História da Computação Aplicada no Brasil

    Directory of Open Access Journals (Sweden)

    Adelaine Gelain

    2014-11-01

    Full Text Available A área de pesquisa da Computação Aplicada (CA no Brasil, em fase de consolidação, tem crescido em importância graças à sua relação com outras áreas. Por sua “jovialidade”, pouco se tem analisado sobre a forma como se faz pesquisas nessa área. A Revista Brasileira de Computação Aplicada (RBCA é uma publicação representativa desse foco que foi criada há pouco tempo. Então, para se ter uma visão das pesquisas na área de CA analisou-se a produção registrada na RBCA. Três análises foram feitas: quanto aos paradigmas científicos predominantes, estilo de maturidade e abordagem de pesquisa das publicações dos últimos cinco anos. Um checklist foi concebido para facilitar a análise mais subjetiva – do paradigma. Todos os artigos foram avaliados ao menos duas vezes, sendo que a maioria sofreu três avaliações distintas. As 501 avaliações em três rodadas permitiu caracterizar a produção como majoritariamente tecnocrática, usando estilos de maturidade iniciais e abordagens desenvolvimentista e descritiva. Esses elementos sugerem que os pesquisadores da área adotam o viés das Engenharias, o que é razoável considerando o aspecto “aplicado”. Entretanto, para a CA consolidar-se como uma ramificação da Ciência da Computação (CC, vê-se importante que os pesquisadores da CA utilizem metodologias científicas de maior credibilidade.

  14. Update on Intra-Arterial Chemotherapy for Retinoblastoma

    Directory of Open Access Journals (Sweden)

    Mario Zanaty

    2014-01-01

    Full Text Available The tools for managing retinoblastoma have been increasing in the past decade. While globe-salvage still relies heavily on intravenous chemotherapy, tumors in advanced stage that failed chemotherapy are now referred for intra-arterial chemotherapy (IAC to avoid enucleation. However, IAC still has many obstacles to overcome. We present an update on the indications, complications, limitations, success, and technical aspects of IAC. Given its safety and high efficacy, it is expected that IAC will replace conventional strategies and will become a first-line option even for tumors that are amenable for other strategies.

  15. Diagnóstico y tratamiento de retinoblastoma

    OpenAIRE

    Nie Meiting

    2012-01-01

    El retinoblastoma es el tumor maligno más frecuente en la infancia, sin embargo sigue siendo un tumor raro en niños. Se asocia a una inactivación de ambos alelos del gen supresor tumoral. Pueden aparecer esporádicamente o pueden ser familiares y existen varios patrones de diseminación tumoral que pueden variar el pronóstico tanto visual como del globo ocular. El signo más frecuente es la leucocoria seguido por estrabismo, con una edad promedio de presentación a los 2 años en casos unilaterale...

  16. Association of Electroencephalography (EEG) Power Spectra with Corneal Nerve Fiber Injury in Retinoblastoma Patients.

    Science.gov (United States)

    Liu, Jianliang; Sun, Juanjuan; Diao, Yumei; Deng, Aijun

    2016-09-04

    BACKGROUND In our clinical experience we discovered that EEG band power may be correlated with corneal nerve injury in retinoblastoma patients. This study aimed to investigate biomarkers obtained from electroencephalography (EEG) recordings to reflect corneal nerve injury in retinoblastoma patients. MATERIAL AND METHODS Our study included 20 retinoblastoma patients treated at the Department of Ophthalmology, Affiliated Hospital of Weifang Medical University between 2010 and 2014. Twenty normal individuals were included in the control group. EEG activity was recorded continuously with 32 electrodes using standard EEG electrode placement for detecting EEG power. A cornea confocal microscope was used to examine corneal nerve injury in retinoblastoma patients and normal individuals. Spearman rank correlation analysis was used to analyze the correlation between corneal nerve injury and EEG power changes. The sensitivity and specificity of changed EEG power in diagnosis of corneal nerve injury were also analyzed. RESULTS The predominantly slow EEG oscillations changed gradually into faster waves in retinoblastoma patients. The EEG pattern in retinoblastoma patients was characterized by a distinct increase of delta (PEEG spectra power and negatively correlated with theta EEG spectra power. The diagnostic sensitivity and specificity by compounding in the series were 60% and 67%, respectively. CONCLUSIONS Changes in delta and theta of EEG appear to be associated with occurrence of corneal nerve injury. Useful information can be provided for evaluating corneal nerve damage in retinoblastoma patients through analyzing EEG power bands.

  17. Genetic Profile of Retinoblastoma Patients from a Referral Hospital in Gujarat

    Directory of Open Access Journals (Sweden)

    Sunil G Oza

    2012-06-01

    Full Text Available Background & objectives: Retinoblastoma is the most common intraocular malignancy of infant and childhood. It is the first disease for which a genetic etiology of cancer has been described and the first suppressor gene identified. The chromosomal abnormality found in retinoblastoma cases is deletion of 13q14. The aim of this study was to carry out karyotypic study in Retinoblastoma cases and identifying nature of chromosomal abnormalities in these patients to determine recurrence risks to assist genetic counseling. Materials and Methods: Karyotypic study was perform by Trypsin- Giemsa Banding for 17 unilateral and 8 bilateral retinoblastoma patients (total 25 for that their blood samples were taken. A prior written consent was taken from the parents of these patients. Results: Out of 25 cases of Retinoblastoma, one female (4% had 13q14 deletion and 24 (96% showed normal chromosomal constitution and not a single case of translocation and mosaicism were found. Conclusion: Karyotyping is the simplest and affordable genetic test for most of the retinoblastoma families especially in a developing country like India. Genetic testing is crucial for accurate risk prediction for retinoblastoma in close relatives of probands and provides a basis for genetic counseling. [National J of Med Res 2012; 2(3.000: 322-324

  18. Dosimetric Study of Current Treatment Options for Radiotherapy in Retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Eldebawy, Eman [Department of Radiation Oncology, McGill University Health Centre, Montreal, Quebec (Canada); Department of Radiation Oncology, Children' s Cancer Hospital, Cairo (Egypt); Parker, William, E-mail: william.parker@mcgill.ca [Department of Medical Physics, McGill University Health Centre, Montreal, Quebec (Canada); Abdel Rahman, Wamied [Department of Medical Physics, McGill University Health Centre, Montreal, Quebec (Canada); Freeman, Carolyn R. [Department of Radiation Oncology, McGill University Health Centre, Montreal, Quebec (Canada)

    2012-03-01

    Purpose: To determine the best treatment technique for patients with retinoblastoma requiring radiotherapy to the whole eye. Methods and Materials: Treatment plans for 3 patients with retinoblastoma were developed using 10 radiotherapy techniques including electron beams, photon beam wedge pair (WP), photon beam three-dimensional conformal radiotherapy (3D-CRT), fixed gantry intensity-modulated radiotherapy (IMRT), photon volumetric arc therapy (VMAT), fractionated stereotactic radiotherapy, and helical tomotherapy (HT). Dose-volume analyses were carried out for each technique. Results: All techniques provided similar target coverage; conformity was highest for VMAT, nine-field (9F) IMRT, and HT (conformity index [CI] = 1.3) and lowest for the WP and two electron techniques (CI = 1.8). The electron techniques had the highest planning target volume dose gradient (131% of maximum dose received [D{sub max}]), and the CRT techniques had the lowest (103% D{sub max}) gradient. The volume receiving at least 20 Gy (V{sub 20Gy}) for the ipsilateral bony orbit was lowest for the VMAT and HT techniques (56%) and highest for the CRT techniques (90%). Generally, the electron beam techniques were superior in terms of brain sparing and delivered approximately one-third of the integral dose of the photon techniques. Conclusions: Inverse planned image-guided radiotherapy delivered using HT or VMAT gives better conformity index, improved orbital bone and brain sparing, and a lower integral dose than other techniques.

  19. UNILATERAL SPORADIC RETINOBLASTOMA IN AN 11-YEAR-OLD CHILD: A RARE CASE REPORT

    Directory of Open Access Journals (Sweden)

    Nisha Jha

    2016-06-01

    Full Text Available BACKGROUND Retinoblastoma is the commonest intraocular tumour of childhood. Majority of cases are diagnosed before 5 years of age, with late presentation being a rarity. CASE REPORT An 11-year-old boy presented to our OPD with diminution of vision and white pupillary reflex in the left eye since 1 month. Right eye was normal. Fundus examination of left eye revealed a mass in the inferior quadrant with vitreous haemorrhage. Bscan and MRI were suggestive of retinoblastoma and showed no signs of optic nerve involvement. Subsequently, the patient underwent enucleation of left eye. Primary orbital implant was given. Diagnosis was confirmed histopathologically. CONCLUSION Late presentation of retinoblastoma though uncommon can cause acute visual impairment. Retinoblastoma should be considered in the differential diagnosis of leukocoria in any age group.

  20. Inteligência Competitiva: metodologias aplicadas em empresas brasileirasInteligencia Competitiva: metodologías aplicadas en las empresas brasileñas

    Directory of Open Access Journals (Sweden)

    Mônica Erichsen Nassif

    2012-01-01

    Full Text Available Introdução: Apresenta um panorama acerca da Inteligência Competitiva no contexto das organizações brasileiras. Objetivo: Investiga a existência de metodologias de Inteligência Competitiva aplicadas a organizações nacionais e multinacionais de diferentes segmentos de mercado. Metodologia: Revisão de literatura nacional e internacional – envolvendo o status quo da atividade de Inteligência Competitiva no mundo – e cujo conteúdo foi confrontado com os resultados obtidos em entrevistas realizadas em 13 organizações nacionais e multinacionais com atuação no Brasil, no período de dezembro de 2010 a abril de 2011. Resultados: No artigo foram analisados os diferentes objetivos e experiências de Inteligência Competitiva em organizações envolvendo diferentes práticas para a projeção de cenários, análise de conteúdos provenientes de mídias e monitoramento do ambiente geral e setorial das organizações. A pesquisa apontou ainda as principais fontes e produtos de informação, perfil dos participantes e ferramentas utilizadas para a prática de Inteligência Competitiva no ambiente organizacional brasileiro. Conclusões: Embora as organizações adotem metodologias relativamente similares de Inteligência Competitiva, essa atividade apresenta objetivos distintos, afeitos tanto ao enfoque estratégico, quanto aos enfoques mercadológico, comercial e de vendas, prevalecendo o primeiro. O aspecto estratégico também está refletido nos modelos de subordinação hierárquica dominante das áreas de Inteligência Competitiva.

  1. Tratamento conservador em retinoblastoma intra-ocular Conservative treatment for intraocular retinoblastoma

    Directory of Open Access Journals (Sweden)

    Clélia Maria Erwenne

    2003-12-01

    bilaterais foram conservados 48,9% dos olhos tratados. Entre os unilaterais, para os mesmos estádios obteve-se: I 0/0; II 2/2 (100,0%; III 1/3 (33,3%; IV 1/3 (33,3%; V 0/7 (0%. Considerando-se o total de unilaterais foram conservados 26,6% dos olhos. Não se observou nefro ou ototoxicidade às drogas neste grupo.PURPOSE: To report the results of conservation of the ocular globe after treatment with carboplatin and laser; with or without the use of other forms of local treatment or radiotherapy, if necessary. METHODS: All eyes, initially untreated, were evaluated using indirect ophthalmology under inhalation anesthesia in an outpatient clinic treatment setting. They were categorized according to the Reese-Ellsworth system; thereafter, the patients were evaluated clinically by a pediatric oncologist. The patients received the first cycle of chemotherapy consisting of intravenous carboplatin 200 mg/m², vincristine 1.5 mg/m² and etoposide 150 mg/m², for three days. This was followed by other cycles with intervals of between 21 and 28 days. From 7 to 15 days after the end of the chemotherapy cycle, another indirect ophthalmoscopy under inhalation anesthesia was performed, with documentation of the findings and laser hyperthermia application, using a Diode laser, 810 nm, continuous pulse with a large spot size. The procedure (chemotherapy and laser was repeated until complete cicatrization of all lesions was observed, or until the need for another treatment modality such as radiotherapy or enucleation became apparent. Follow-up examinations every two months during the first year and three months thereafter were done for review and for treatment of recurrent or new lesions. Pediatric oncology follow-up was also done throughout the entire initial assessment and follow-up period. RESULTS: We treated a total of 62 patients with retinoblastoma, of whom 32 were male and 30 female. Fifteen were unilateral and 47 were bilateral with a total of 107 eyes. The mean age was 16.9 months (2

  2. Establishment of Retinoblastoma Model in NOD-SCID Mice and Study of Metastasis

    Institute of Scientific and Technical Information of China (English)

    Bo Zhang; Yongping Li; Xiufeng Zhong; Wenge Huang; Li Nie; Wenxin Zhang

    2005-01-01

    Purpose: To establish model of retinoblastoma subcutaneously in NOD-SCID mice and study rules of formation and distribution of retinoblastoma metastasis.Methods: Retinoblastoma cells SO-RB50 were inoculated subcutaneously in NOD-SCID mice. Animal acts and tumor formation, growth and metastasis in NOD-SCID mice were observed. Primary and metastatic tumors were studied pathohistologically by HE and immunohistochemical staining.Results: The latent periods of tumor growth were 12~19 days and the taken rate of tumor was 100%. 32 days later, 5 NOD-SCID mice were found with tumors that had metastasized to areas mainly located in the abdominal cavity and the side of the kidney; the metastatic time of tumors in the mice also differed. The tumor cells of the primary nodules and the metastasis were similar with human retinoblastoma cells and positive in immunohistochemical staining of NSE.Conclusion: The subcutaneous model of retinoblastoma in NOD-SCID mice showed a high taken rate and a short latent period of tumor, which had a high metastatic rate and was the best model in research of behaviors of retinoblastoma at present.

  3. A Study of Immunoactivity of Retinal S—Antigen in Retinoblastoma

    Institute of Scientific and Technical Information of China (English)

    YueSong; GuangdaYao

    1995-01-01

    Purposese:To study retinal S-antigen expression in human retinoblastoma and as-sess if there is a correlation between S-antigen immunoactivity and degree of retinoblastoma cell differentiations.Methods:Ten cases of Chinese retinoblastoma parafin-embedded tissues were ap-plied for this thudy.A strain of monoclonal antibody,MabA9C6,Which defines an epitope in S-antigen retained in fixed-tissue sections,was used to study S-antigen expression in 10 cases of retinoblastomas.S-antigen was localized by the biotina-vidin indirect immunoperoxidase technique and purified MabA9C6 ascites fluid was used with1100dilution.The whole procedure could be finished within a few hours.Results:The S-antigen immunosctivity was observed in different pattterns:the“normal”photorecepto elements incorporated in 3cases of growing tumors;3of 4Fleurettes and E-W rosettes;and scattered tumor cells in50%of the cases.Conclusions:The result suggests that the expression of S-antigen in retinoblas-toma may be used to assess the degree of tumor differentiation as anothe tumor marker in retinoblastoma.

  4. Genetic perspective of retinoblastoma: From present to future

    Directory of Open Access Journals (Sweden)

    Madhavan Jagadeesan

    2016-01-01

    Full Text Available Retinoblastoma (RB is the most common malignant intraocular tumor in children. In the last decade, basic research has led to a better understanding of events after two hits in RB susceptibility gene (RB1, molecular mechanism of tumor growth, the cell of origin of RB, etc. This would pave way to identify biomarkers and molecular targeted therapy for better treatment option in the future. Furthermore, improvement in molecular techniques has led to enhanced diagnostic methods for early diagnosis, genetic counseling, and prevention of the disease. This review will help to understand the essence of basic research work conducted in recent times and its implication in the management of RB in the future.

  5. Radiation regression patterns after cobalt plaque insertion for retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Buys, R.J.; Abramson, D.H.; Ellsworth, R.M.; Haik, B.

    1983-08-01

    An analysis of 31 eyes of 30 patients who had been treated with cobalt plaques for retinoblastoma disclosed that a type I radiation regression pattern developed in 15 patients; type II, in one patient, and type III, in five patients. Nine patients had a regression pattern characterized by complete destruction of the tumor, the surrounding choroid, and all of the vessels in the area into which the plaque was inserted. This resulting white scar, corresponding to the sclerae only, was classified as a type IV radiation regression pattern. There was no evidence of tumor recurrence in patients with type IV regression patterns, with an average follow-up of 6.5 years, after receiving cobalt plaque therapy. Twenty-nine of these 30 patients had been unsuccessfully treated with at least one other modality (ie, light coagulation, cryotherapy, external beam radiation, or chemotherapy).

  6. A multicentre report from the Mexican Retinoblastoma Group

    Science.gov (United States)

    Leal-Leal, C; Flores-Rojo, M; Medina-Sansón, A; Cerecedo-Díaz, F; Sánchez-Félix, S; González-Ramella, O; Pérez-Pérez, F; Gómez-Martínez, R; Quero-Hernández, A; Altamirano-Álvarez, E; Alejo-González, F; Figueroa-Carbajal, J; Ellis-Irigoyen, A; Tejocote-Romero, I; Cervantes-Paz, R; Pantoja-Guillén, F; Vega-Vega, L; Carrete-Ramírez, F

    2004-01-01

    Background: Retinoblastoma (RB) is a relatively uncommon tumour in childhood. The incidence of retinoblastoma in Mexico is probably higher than the incidence reported worldwide, however there is not enough information about the characteristics of this illness in Mexico. This report aims to present the results of a multicentre clinical survey of RB in Mexico. Methods: A retrospective study was carried out on all RB cases treated in 16 institutions during the last six years. The variables analysed were age at diagnosis, sex, affected eyes, treatment modalities, and pathological staging. Overall survival was obtained. Results: The authors analysed 500 cases; age range was 0–182 months. There were 364 unilateral cases (72.8%). Enucleation was performed in 84.9% of the patients. The St Jude’s staging was: 7.4% stage I, 52.8% stage II, 18.0% stage III, 11.4% stage IV, 7.2% not evaluated, and 3.2% missing data. Chemotherapy was used in 74.4% of the patients. Disease free survival was 89% at 73 months follow up. Conclusions: The paper presents a great number of cases and pioneers multicentre studies in paediatric ophthalmology and oncology in this country. Given the great number of patients in advanced stages and the variability on treatment schemes, it is evident that it is mandatory to work in a cooperative group and develop a national early detection programme as well as a treatment protocol which include all specialists involved in the care of patients with RB. PMID:15258028

  7. Treatment of retinoblastoma by precision megavoltage radiation therapy

    Energy Technology Data Exchange (ETDEWEB)

    Schipper, J.; Peperzeel, H.A. van (Rijksuniversiteit Utrecht (Netherlands). Academisch Ziekenhuis); Tan, K.E.W.P. (Royal Dutch Eye Hospital, Utrecht, Netherlands)

    1985-02-01

    The principal treatment concept in the Utrecht Retinoblastoma Centre is megavoltage irradiation, followed by light coagulation and/or cryotherapy if there is any doubt as to whether the residual tumour is still active. Radiation therapy is administered by means of a simple but highly accurate temporal beam technique. A standardized dose of 45 Gy is given in 15 fractions of 3 Gy at 3 fractions per week. From 1971 to 1982, 39 children with retinoblastoma have been irradiated in at least one eye. Of the 73 affected eyes, 18 were primarily enucleated, one received light coagulation only, and 54 received radiation therapy. Of the 54 irradiated eyes, 32 were additionally treated by light coagulation and/or cryotherapy for suspicious residual tumour (in 29 eyes), recurrent tumour (in 1 eye), and/or new tumour (in 3 eyes) and 10 were ultimately enucleated. Two eyes also received hyperthermia. The percentages of cure of the irradiated eyes with a minimum follow-up of 2 years were 100% (14/14), 100% (9/9), 83% (10/12), 79% (11/14) and 0% (0/5) in the Reese-Ellsworth groups I to V-A, respectively. Of the saved eyes 95% achieved useful vision. Eighteen eyes developed a clinically detectable radiation cataract; in five of these the lens was aspirated. Cataracts developed exclusively in those lenses of which a posterior portion of more than 1 mm had to be included in the treatment field. The likelihood and the degree of cataract formation was found to be directly related to the dose of radiation to the germinative zone of the lens epithelium. The minimum cataractogenic dose found in this series was 8 Gy.

  8. Treatment of retinoblastoma: current status and future perspectives.

    Science.gov (United States)

    Rodriguez-Galindo, Carlos; Chantada, Guillermo L; Haik, Barrett G; Wilson, Matthew W

    2007-07-01

    Treatment of retinoblastoma must be individualized. Most patients with unilateral, non-metastatic retinoblastoma can be cured with enucleation alone. In patients with histologic risk factors, adjuvant chemotherapy is recommended, with the addition of orbital radiation for patients with trans-scleral involvement or tumor present at the level of the cut end of the optic nerve. Patients with metastases require intensive chemotherapy and consolidation with autologous hematopoietic stem cell rescue. Patients with bilateral or multifocal disease represent a major challenge. Cure of the disease is the first priority, but the therapeutic approach also has to consider eye and vision preservation. The approach is conservative, and only eyes with very advanced disease are enucleated upfront. Patients are treated with chemotherapy and intensive focal treatments, with the aim of delaying or avoiding radiation therapy and enucleation. For patients with early intraocular stage (Reese-Ellsworth groups I-III and International Groups A-B), the two-drug combination of vincristine and carboplatin is recommended. Patients with more advanced intraocular disease (Reese-Ellsworth groups IV-V and International Groups C-D) require more intensive chemotherapy. Standard of care for these patients incorporates etoposide into the regimen. Effective agents with good intraocular penetration, such as topotecan, are being investigated. Because most failures are secondary to progression of the vitreous seeds, subconjunctival carboplatin is added in cases with poor response of the vitreous tumors. Patients must be monitored very closely, with examinations under anesthesia every 4 to 6 weeks, and focal treatments are applied during the procedure. These include cryotherapy for small anterior tumors, thermotherapy and laser photocoagulation for small posterior tumors, and brachytherapy for larger tumors. New treatment approaches under development include the refinement of periocular chemotherapy

  9. Información sobre las secuelas y futuros riesgos para los sobrevivientes de retinoblastoma Information on late effects and future risks for retinoblastoma survivors

    OpenAIRE

    Laura Schwartz

    2005-01-01

    Los índices de sobrevida de los pacientes con cáncer en la infancia han aumentado en las últimas décadas. Se observan secuelas como consecuencia de esta enfermedad y su tratamiento. Los sobrevivientes de retinoblastoma presentan efectos adversos como consecuencia de la cirugía, la radioterapia y la quimioterapia. Las formas bilaterales irradiadas presentan un riesgo aumentado de desarrollar un segundo cáncer. Tanto los casos que presentaron retinoblastoma unilateral o bilateral pueden transmi...

  10. Estadística aplicada a Psicología

    OpenAIRE

    2012-01-01

    Este documento es un curso de introducción a Estadística aplicada al análisis de datos en Psicología. Está estructurado en dos bloques de temas de Estadística Descriptiva e Inferencial, que incluyen contenidos teóricos y ejercicios. La página de inicio da acceso a cada bloque, y una vez dentro se accede a los contenidos a través de un menú desplegable. Curs d’introducció a Estadística aplicada a l’anàlisi de dades en Psicologia. Els temes estan agrupats en dos blocs, un per Estadística Des...

  11. Información sobre las secuelas y futuros riesgos para los sobrevivientes de retinoblastoma Information on late effects and future risks for retinoblastoma survivors

    Directory of Open Access Journals (Sweden)

    Laura Schwartz

    2005-04-01

    Full Text Available Los índices de sobrevida de los pacientes con cáncer en la infancia han aumentado en las últimas décadas. Se observan secuelas como consecuencia de esta enfermedad y su tratamiento. Los sobrevivientes de retinoblastoma presentan efectos adversos como consecuencia de la cirugía, la radioterapia y la quimioterapia. Las formas bilaterales irradiadas presentan un riesgo aumentado de desarrollar un segundo cáncer. Tanto los casos que presentaron retinoblastoma unilateral o bilateral pueden transmitir esta enfermedad a su descendencia. El diagnóstico de este tumor es excepcional después de los 4 años de edad y no se sabe claramente qué conocimiento tienen estos sobrevivientes del diagnóstico, del tratamiento y de los futuros riesgos. El consentimiento informado y el posterior resumen de historia clínica no aclaran demasiado a los padres. Los pacientes curados de retinoblastoma se verían beneficiados con un seguimiento prolongado en las instituciones en que fueron tratados, ya que les permitiría recibir información acerca de lo padecido, del tratamiento, de los riesgos y se podrían identificar las consecuencias posteriores de la enfermedad y su tratamiento.The survival rates of childhood cancer have increased in the past few decades. Late consequences related to the cancer and the treatment are observed. The late effects in retinoblastoma survivors are related to the surgery, the radiotherapy and the chemotherapy. Patients with irradiated bilateral retinoblastoma are at high risk to develop a second cancer. Survivors of bilateral or unilateral forms could transmit this disease to their offspring. The diagnosis of retinoblastoma is exceptional after 4 years of age, and it is not clear whether these survivors have knowledge of their diagnosis, treatment and future risks. The informed consent and the summary of the clinical histories do not help to clarify the situation. It will be of great benefit for the patients cured of retinoblastoma

  12. AO operations at Gemini South

    Science.gov (United States)

    Marin, Eduardo; Cardwell, Andrew; Pessev, Peter

    2014-08-01

    The 8m Gemini South telescope is entering an exciting new era of AO operations, which put it at the forefront of astronomical AO in terms of both wide field AO, and extreme-AO systems. Major milestones achieved were the successful commissioning of GeMS, in 2012, and GPI, in late 2013 and early 2014. Currently we are operating two of the worlds most advanced astronomical AO systems. Gemini, running primarily in queue, must balance the promise of AO with the demands of the community to use non-AO instruments. We discuss the current state of the two AO systems, and their operational models. The preparations that go into planning each AO run, the difficulties in scheduling around non-AO instruments, and the differences between scheduling LGS AO and non-LGS AO are discussed.

  13. Crystal Structure of Human Retinoblastoma Binding Protein 9

    Energy Technology Data Exchange (ETDEWEB)

    Vorobiev, S.; Su, M; Seetharaman, J; Huang, Y; Chen, C; Maglaqui, M; Janjua, H; Montelione, G; Tong, L; et. al.

    2009-01-01

    As a step towards better integrating protein three-dimensional (3D) structural information in cancer systems biology, the Northeast Structural Genomics Consortium (NESG) (www.nesg.org) has constructed a Human Cancer Pathway Protein Interaction Network (HCPIN) by analysis of several classical cancer-associated signaling pathways and their physical protein-protein interactions. Many well-known cancer-associated proteins play central roles as hubs or bottlenecks in the HCPIN (http://nmr.cabm.rutgers.edu/hcpin). NESG has selected more than 1000 human proteins and protein domains from the HCPIN for sample production and 3D structure determination. The long-range goal of this effort is to provide a comprehensive 3D structure-function database for human cancer-associated proteins and protein complexes, in the context of their interaction networks. Human retinoblastoma binding protein 9 (RBBP9) is one of the HCPIN proteins targeted by NESG. RBBP9 was initially identified as the product of a new gene, Bog (for B5T over-expressed gene), in several transformed rat liver epithelial cell lines resistant to the growth-inhibitory effect of TGF-1 as well as in primary human liver tumors. RBBP9 contains the retinoblastoma (Rb) binding motif LxCxE in its sequence, and was shown to interact with Rb by yeast two-hybrid and coimmunoprecipitation experiments. Mutation of the Leu residue in this motif to Gln blocked the binding to Rb. RBBP9 can displace E2F1 from E2F1-Rb complexes, and over expression of RBBP9 overcomes TGF-1 induced growth arrest and results in transformation of rat liver epithelial cells leading to hepatoblastoma-like tumors in nude mice. RBBP9 may also play a role in cellular responses to chronic low dose radiation. A close homolog of RBBP9, sharing 93% amino acid sequence identity and also known as RBBP10, interacts with a protein with sua5-yciO-yrdC domains.

  14. Characteristics of an Established Retinoblastoma Cell Line HXO—Rb44

    Institute of Scientific and Technical Information of China (English)

    HepingXu; HechengZhu

    1995-01-01

    Purpose:To study the retinoblastoma cell culture and to establish a new retinoblastoma cellline.Methods:22 retinoblastomes were cultured by using the method of single cellsus-pension.Characteristics of the cultured cells were studied in the following pro-grams:tumor cell morphology in vitro,electron microscopic,growth curve,cloning in soft agar,immunohistochemistry,karyotype and tumorigenicity.Results.22 retinoblastoas were cultured successfully in ivtro,only a cotinued cell line HXO-Rb44was established(more than3years).The characteristics of this cell line are that it grew as a suspension of round cell in graps like clusters in vitro,its population doubling time was 44hours,and it could be cloned in softa-gar.Histopathologic and ulatastructured pictures showed the characteristics of Rb.HXO-Rb44cell was positive to NSE and negative to GFAP in immunohis-tochemical staining.A subcutaneous injection of HXO-Rb44cells produced a retinoblastoma in BALB/C athumic nude mice.Conclusions:HXO-Rb44 has the characteristice of retinoblastoma and is a new retinoblastoma cell line.It is a useflu material for study this tumor both in basic and clinical fields.

  15. Forced Trefoil Factor Family Peptide 3 (TFF3) Expression Reduces Growth, Viability, and Tumorigenicity of Human Retinoblastoma Cell Lines.

    Science.gov (United States)

    Große-Kreul, Jan; Busch, Maike; Winter, Claudia; Pikos, Stefanie; Stephan, Harald; Dünker, Nicole

    2016-01-01

    Trefoil factor family (TFF) peptides have been shown to effect cell proliferation, apoptosis, migration and invasion of normal cells and various cancer cell lines. In the literature TFF peptides are controversially discussed as tumor suppressors and potential tumor progression factors. In the study presented, we investigated the effect of TFF3 overexpression on growth, viability, migration and tumorigenicity of the human retinoblastoma cell lines Y-79, WERI-Rb1, RBL-13 and RBL-15. As revealed by WST-1 and TUNEL assays as well as DAPI and BrdU cell counts, recombinant human TFF3 significantly lowers retinoblastoma cell viability and increases apoptosis levels. Transient TFF3 overexpression likewise significantly increases RB cell apoptosis. Stable, lentiviral TFF3 overexpression lowers retinoblastoma cell viability, proliferation and growth and significantly increases cell death in retinoblastoma cells. Blockage experiments using a broad-spectrum caspase inhibitor and capase-3 immunocytochemistry revealed the involvement of caspases in general and of caspase-3 in particular in TFF3 induced apoptosis in retinoblastoma cell lines. Soft agarose and in ovo chicken chorioallantoic membrane (CAM) assays revealed that TFF3 overexpression influences anchorage independent growth and significantly decreases the size of tumors forming from retinoblastoma cells. Our study demonstrates that forced TFF3 expression exerts a significant pro-apoptotic, anti-proliferative, and tumor suppressive effect in retinoblastoma cells, setting a starting point for new additive chemotherapeutic approaches in the treatment of retinoblastoma.

  16. Forced Trefoil Factor Family Peptide 3 (TFF3) Expression Reduces Growth, Viability, and Tumorigenicity of Human Retinoblastoma Cell Lines

    Science.gov (United States)

    Winter, Claudia; Pikos, Stefanie; Stephan, Harald; Dünker, Nicole

    2016-01-01

    Trefoil factor family (TFF) peptides have been shown to effect cell proliferation, apoptosis, migration and invasion of normal cells and various cancer cell lines. In the literature TFF peptides are controversially discussed as tumor suppressors and potential tumor progression factors. In the study presented, we investigated the effect of TFF3 overexpression on growth, viability, migration and tumorigenicity of the human retinoblastoma cell lines Y-79, WERI-Rb1, RBL-13 and RBL-15. As revealed by WST-1 and TUNEL assays as well as DAPI and BrdU cell counts, recombinant human TFF3 significantly lowers retinoblastoma cell viability and increases apoptosis levels. Transient TFF3 overexpression likewise significantly increases RB cell apoptosis. Stable, lentiviral TFF3 overexpression lowers retinoblastoma cell viability, proliferation and growth and significantly increases cell death in retinoblastoma cells. Blockage experiments using a broad-spectrum caspase inhibitor and capase-3 immunocytochemistry revealed the involvement of caspases in general and of caspase-3 in particular in TFF3 induced apoptosis in retinoblastoma cell lines. Soft agarose and in ovo chicken chorioallantoic membrane (CAM) assays revealed that TFF3 overexpression influences anchorage independent growth and significantly decreases the size of tumors forming from retinoblastoma cells. Our study demonstrates that forced TFF3 expression exerts a significant pro-apoptotic, anti-proliferative, and tumor suppressive effect in retinoblastoma cells, setting a starting point for new additive chemotherapeutic approaches in the treatment of retinoblastoma. PMID:27626280

  17. Retinoblastoma: Achieving new standards with methods of chemotherapy

    Directory of Open Access Journals (Sweden)

    Swathi Kaliki

    2015-01-01

    Full Text Available The management of retinoblastoma (RB has dramatically changed over the past two decades from previous radiotherapy methods to current chemotherapy strategies. RB is a remarkably chemotherapy-sensitive tumor. Chemotherapy is currently used as a first-line approach for children with this malignancy and can be delivered by intravenous, intra-arterial, periocular, and intravitreal routes. The choice of route for chemotherapy administration depends upon the tumor laterality and tumor staging. Intravenous chemotherapy (IVC is used most often in bilateral cases, orbital RB, and as an adjuvant treatment in high-risk RB. Intra-arterial chemotherapy (IAC is used in cases with group C or D RB and selected cases of group E tumor. Periocular chemotherapy is used as an adjunct treatment in eyes with group D and E RB and those with persistent/recurrent vitreous seeds. Intravitreal chemotherapy is reserved for eyes with persistent/recurrent vitreous seeds. In this review, we describe the various forms of chemotherapy used in the management of RB. A database search was performed on PubMed, using the terms "RB," and "treatment," "chemotherapy," "systemic chemotherapy," "IVC," "IAC," "periocular chemotherapy," or "intravitreal chemotherapy." Relevant English language articles were extracted, reviewed, and referenced appropriately.

  18. Regulation of the retinoblastoma proteins by the human herpesviruses

    Directory of Open Access Journals (Sweden)

    Kalejta Robert F

    2009-01-01

    Full Text Available Abstract Viruses are obligate intracellular parasites that alter the environment of infected cells in order to replicate more efficiently. One way viruses achieve this is by modulating cell cycle progression. The main regulators of progression out of G0, through G1, and into S phase are the members of the retinoblastoma (Rb family of tumor suppressors. Rb proteins repress the transcription of genes controlled by the E2F transcription factors. Because the expression of E2F-responsive genes is required for cell cycle progression into the S phase, Rb arrests the cell cycle in G0/G1. A number of viral proteins directly target Rb family members for inactivation, presumably to create an environment more hospitable for viral replication. Such viral proteins include the extensively studied oncoproteins E7 (from human papillomavirus, E1A (from adenovirus, and the large T (tumor antigen (from simian virus 40. Elucidating how these three viral proteins target and inactivate Rb has proven to be an invaluable approach to augment our understanding of both normal cell cycle progression and carcinogenesis. In addition to these proteins, a number of other virally-encoded inactivators of the Rb family have subsequently been identified including a surprising number encoded by human herpesviruses. Here we review how the human herpesviruses modulate Rb function during infection, introduce the individual viral proteins that directly or indirectly target Rb, and speculate about what roles Rb modulation by these proteins may play in viral replication, pathogenesis, and oncogenesis.

  19. The genetic alteration of retinoblastoma gene in esophageal cancer

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Jae Il; Shim, Yung Mok; Kim, Chang Min [Korea Cancer Center Hospital of Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1994-12-01

    Retinoblastoma(RB) gene is the prototype of tumor suppressor gene and it`s alteration have been frequently observed in a large number of human tumors. To investigate the role of RB in esophageal cancer, we studied 36 esophageal cancer tissues with Southern blot analysis to detect gross LOH and PCR-SSCP method to find minute LOH and mutation, if any. In the cases with abnormalities, the nucleotide sequence analysis was performed. Allelic loss of chromosome 13q14 occurred in 20 out of 32 informative cases (62.5%) by Southern analysis. Furthermore, PCR-LOH added three positive cases. Mobility shift by PCR-SSCP was observed in one case at exon 22, which showed 1 bp deletion in codon 771 of RB gene resulting in frame shift mutation. Besides, nine PCR-band alteration in tumor tissue compared with normal tissue were observed in exon 14 and 22, but mutation was not found on sequencing analysis suggesting the epigenetic alteration in tumor tissue. Analysis of the clinical data did not show any difference depending upon RB alteration. However, the total incidence of RB gene may play an important role in the development of esophageal cancer. The main genetic alteration of RB gene was deletion detected by Southern blot and one bp deletion leading to frame shift was also observed. 8 figs, 5 tabs. (Author).

  20. Retinoblastoma loss modulates DNA damage response favoring tumor progression.

    Directory of Open Access Journals (Sweden)

    Marcos Seoane

    Full Text Available Senescence is one of the main barriers against tumor progression. Oncogenic signals in primary cells result in oncogene-induced senescence (OIS, crucial for protection against cancer development. It has been described in premalignant lesions that OIS requires DNA damage response (DDR activation, safeguard of the integrity of the genome. Here we demonstrate how the cellular mechanisms involved in oncogenic transformation in a model of glioma uncouple OIS and DDR. We use this tumor type as a paradigm of oncogenic transformation. In human gliomas most of the genetic alterations that have been previously identified result in abnormal activation of cell growth signaling pathways and deregulation of cell cycle, features recapitulated in our model by oncogenic Ras expression and retinoblastoma (Rb inactivation respectively. In this scenario, the absence of pRb confers a proliferative advantage and activates DDR to a greater extent in a DNA lesion-independent fashion than cells that express only HRas(V12. Moreover, Rb loss inactivates the stress kinase DDR-associated p38MAPK by specific Wip1-dependent dephosphorylation. Thus, Rb loss acts as a switch mediating the transition between premalignant lesions and cancer through DDR modulation. These findings may have important implications for the understanding the biology of gliomas and anticipate a new target, Wip1 phosphatase, for novel therapeutic strategies.

  1. Neurociencia aplicada: el cerebro al servicio de la humanidad

    Directory of Open Access Journals (Sweden)

    Ángel Correa

    2008-04-01

    Full Text Available La neurociencia aplicada utiliza tecnología y conocimiento sobre el cerebro para resolver problemas prácticos, principalmente en la clínica y el trabajo. La cura de lesiones neurológicas, Parkinson y Alzheimer, podría depender de terapias génicas, neurofeedback, neuroestimulación magnética o implantes neurales. En neuroergonomía, la aplicación de la realidad virtual mejora la productividad y la seguridad laboral.

  2. La Antropología Aplicada en una Sociedad Compleja

    Directory of Open Access Journals (Sweden)

    Hugo Cadenas Ramos

    2005-01-01

    Full Text Available El presente artículo reflexiona sobre la definición del término antropología aplicada a la luz de los desafíos que enfrente la disciplina antropológica en el Siglo XXI, desde el punto de vista teórico, metodológico y de aplicación práctica en el más amplio sentido. Si bien el tema está abierto a debate y no se puede llegar a conclusiones taxativas, sí es posible proponer algunas ideas al debate.

  3. Analysis of MDM2 and MDM4 single nucleotide polymorphisms, mRNA splicing and protein expression in retinoblastoma.

    Directory of Open Access Journals (Sweden)

    Justina McEvoy

    Full Text Available Retinoblastoma is a childhood cancer of the developing retina that begins in utero and is diagnosed in the first years of life. Biallelic RB1 gene inactivation is the initiating genetic lesion in retinoblastoma. The p53 gene is intact in human retinoblastoma but the pathway is believed to be suppressed by increased expression of MDM4 (MDMX and MDM2. Here we quantify the expression of MDM4 and MDM2 mRNA and protein in human fetal retinae, primary retinoblastomas, retinoblastoma cell lines and several independent orthotopic retinoblastoma xenografts. We found that MDM4 is the major p53 antagonist expressed in retinoblastoma and in the developing human retina. We also discovered that MDM4 protein steady state levels are much higher in retinoblastoma than in human fetal retinae. This increase would not have been predicted based on the mRNA levels. We explored several possible post-transcriptional mechanisms that may contribute to the elevated levels of MDM4 protein. A proportion of MDM4 transcripts are alternatively spliced to produce protein products that are reported to be more stable and oncogenic. We also discovered that a microRNA predicted to target MDM4 (miR191 was downregulated in retinoblastoma relative to human fetal retinae and a subset of samples had somatic mutations that eliminated the miR-191 binding site in the MDM4 mRNA. Taken together, these data suggest that post-transcriptional mechanisms may contribute to stabilization of the MDM4 protein in retinoblastoma.

  4. Danish retinoblastoma patients 1943-2013 - genetic testing and clinical implications

    DEFF Research Database (Denmark)

    Gregersen, Pernille A; Urbak, Steen F; Funding, Mikkel

    2015-01-01

    of patients diagnosed before DNA testing was offered. Knowledge of heredity increases the chance of early diagnosis in offspring, leading to improved prognosis. We present data from the Danish retinoblastoma patients that emphasize the need for genetic counseling and RB1 screening in all untested......, the rate has been stable around 1 per 14 000 live births with 95% of the patients surviving their retinoblastoma. Stratifying data on the time of diagnosis and status of genetic testing, the number of screened patients gradually increased from 5% in the beginning of the period to 96% in the last five......-year period. A cohort of 181 retinoblastoma survivors with sporadic disease (15% heritable) did not receive genetic testing. Since the introduction of routine testing, one of 14 sporadic unilateral patients tested (7%) has been identified with a germline mutation. Before routine testing, five additional...

  5. Novel function of the retinoblastoma protein in fat: regulation of white versus brown adipocyte differentiation

    DEFF Research Database (Denmark)

    Hansen, Jacob B; te Riele, Hein; Kristiansen, Karsten

    2004-01-01

    The differentiation of white and brown fat cells is controlled by a similar set of transcription factors, including PPARgamma and C/EBPalpha. However, despite many similarities between the two types of fat cells, they carry out essentially opposite functions in vivo, with white adipocytes being...... the major energy store and brown adipocytes being potent energy-dissipaters through thermogenesis. Yet, little is known about factors differentially regulating the formation of white and brown fat cells. Members of the retinoblastoma protein family (pRB, p107, p130) have been implicated in the regulation...... of adipocyte differentiation, and expression and phosphorylation of the three retinoblastoma family proteins oscillate in a characteristic manner during differentiation of the white preadipocyte cell line 3T3-L1. We have recently demonstrated a surprising function of the retinoblastoma protein...

  6. APC/C and retinoblastoma interaction: cross-talk of retinoblastoma protein with the ubiquitin proteasome pathway

    Science.gov (United States)

    Ramanujan, Ajeena; Tiwari, Swati

    2016-01-01

    The ubiquitin (Ub) ligase anaphase promoting complex/cyclosome (APC/C) and the tumour suppressor retinoblastoma protein (pRB) play key roles in cell cycle regulation. APC/C is a critical regulator of mitosis and G1-phase of the cell cycle whereas pRB keeps a check on proliferation by inhibiting transition to the S-phase. APC/C and pRB interact with each other via the co-activator of APC/C, FZR1, providing an alternative pathway of regulation of G1 to S transition by pRB using a post-translational mechanism. Both pRB and FZR1 have complex roles and are implicated not only in regulation of cell proliferation but also in differentiation, quiescence, apoptosis, maintenance of chromosomal integrity and metabolism. Both are also targeted by transforming viruses. We discuss recent advances in our understanding of the involvement of APC/C and pRB in cell cycle based decisions and how these insights will be useful for development of anti-cancer and anti-viral drugs. PMID:27402801

  7. AO Psc time keeping

    Science.gov (United States)

    Bonnardeau, Michel

    2015-07-01

    Eleven seasons, from 2004 to 2014, of photometric monitoring of the intermediate polar AO Psc are presented and are compared with previous observations. The spin up of the white dwarf is found to be slowing down. The amplitudes of the modulated and non-modulated components of the brightness are found to have undergone a major change in 2007.

  8. Meningioma 40 years after radiation therapy for retinoblastoma: genetic and phenotypic analysis, and minireview of literature.

    Science.gov (United States)

    Balik, V; Sarissky, M; Lohmann, D; Sulla, I

    2008-11-01

    The authors present a case of 44-year-old Caucasian female diagnosed with meningothelial meningioma 40 years after radiotherapy for sporadic unilateral retinoblastoma. The genetic analysis of DNA from the meningioma revealed no oncogenic mutation in the RB1 gene. The analysis of meningioma cells by flow cytometry revealed the following immunophenotype: vimentin++ CD56+ GFAP- EGFR-. Intermediate intensities of Her-2/neu and Pgp expression were detected in a small percentage of tumour cells. Data suggest that the tumour was most likely induced by radiotherapy and did not arise as a second tumour as there was no hereditary predisposition to retinoblastoma.

  9. Retinoblastoma: experience of a referral center in the North Region of Portugal

    Directory of Open Access Journals (Sweden)

    Rocha-Bastos R

    2014-05-01

    Full Text Available RA da Rocha-Bastos,1 JR Araújo,1 RS Silva,2 MJ Gil-da-Costa,2 E Brandão,1 NJ Farinha,2,3 F Falcão-Reis,1,4 T Dinah-Bragança1 1Department of Ophthalmology, Hospital São João, 2Hematology and Oncology Unit, Pediatric Hospital, Hospital São João, 3Pediatrics Department, Faculty of Medicine, University of Porto, 4Department of Sense Organs, Faculty of Medicine, University of Porto, Porto, Portugal Purpose: To describe the experience of the Ophthalmology Department of Hospital São João (HSJ, a tertiary health care center in North Region, Portugal, in terms of the diagnosis, treatment, and follow-up of retinoblastoma. Methods: This was a retrospective study of patients diagnosed with retinoblastoma in Hospital São João, between 1978 and 2012. Results: Fifty patients with retinoblastoma were evaluated in our institution between 1978 and 2012. Four patients were excluded due to loss of follow-up. Among the 46 retinoblastoma cases, 33 (71.7% were unilateral and 13 (28.3% bilateral, with a mean age at diagnosis of 22.19 months and 6.92 months, respectively (P<0.001. Leukocoria was the most common presenting sign (36.9%, followed by strabismus (19.6%, a combination of leukocoria and strabismus (8.7%, and buphthalmia (2.2%. Between 1978 and 1992, nine retinoblastoma cases were referred to our hospital, all of them unilateral, and, in each case, enucleation was performed, with or without salvage therapy. Between 1993 and 2012, 18 eyes with retinoblastoma were successfully managed with conservative treatment. Conclusion: Demographic results were generally coincident with previous reports. It is crucial to screen leukocoria in pediatric practice, even in asymptomatic children. The outcome of retinoblastoma treatment in our hospital is similar to other series in developed countries. Keywords: retinoblastoma, leukocoria, strabismus, enucleation, pediatric cancer

  10. Rhegmatogenous retinal detachment in an eye of retinoblastoma treated by radiation and cryocoagulation

    Energy Technology Data Exchange (ETDEWEB)

    Inoue, Makoto; Tanaka, Yasuhiko; Kawai, Masataka; Nii, Seiji [Keio Univ., Tokyo (Japan). School of Medicine; Katsura, Hiroshi; Kaneko, Akihiro

    1999-08-01

    A 4-month-old female infant was diagnosed as bilateral retinoblastoma. The left eye was treated by enucleation and the right by radiation. The right eye received repeated thermochemotherapy, cryocoagulation and photocoagulation for recurrence. Total retinal detachment was detected after surgery for complicated cataract at the age of 2 years 9 months. Vitreous surgery led to the detection of posterior vitreous detachment and retinal breaks in the coagulated scar areas with vitreoretinal adhesion around the calcified tumor. During the following one year, the retina has been attached with no reoccurrence of the tumor. This case illustrates that rhegmatogenous retinal detachment is a possibility in retinoblastoma treated by radiation or retinal coagulations. (author)

  11. Microarray-based analysis: identification of hypoxia-regulated microRNAs in retinoblastoma cells.

    Science.gov (United States)

    Xu, Xiaofang; Jia, Renbing; Zhou, Yixiong; Song, Xin; Wang, Jing; Qian, Guanxiang; Ge, Shengfang; Fan, Xianqun

    2011-05-01

    Hypoxia is an essential feature of retinoblastoma and contributes to poor prognosis and resistance to conventional therapy. MicroRNAs (miRNAs) are small non-coding RNAs involved in a wide variety of biological processes, including cell differentiation, proliferation, death and metabolism. However, the relationship between hypoxia and the expression of miRNAs in retinoblastoma is not well understood. In this study, we aimed to analyze the pattern of miRNA expression in a retinoblastoma cell line under hypoxic conditions and to identify the miRNAs regulated by hypoxia, as well as their possible functions. miRNA expression profiling in retinoblastoma cells (HXO-RB44) under normal and hypoxic conditions was assessed by microarray techniques. The differentially expressed miRNAs were subjected to bioinformatic analyses to predict and categorise the key miRNAs and their target genes. A quantitative real-time RT-PCR approach was used to validate their expression. A Cell Counting kit was used to evaluate the functional significance of miR-181b in RB cell proliferation. There were 46 miRNAs that changed expression more than 2-fold in response to hypoxia (34 up-regulated and 12 down-regulated). We identified a cluster of miRNAs that includes miR-181b, miR-125a-3p, miR-30c-2, miR-497 and miR-491-3p as hypoxia-regulated miRNAs (HRMs) in retinoblastoma cells, of which miR-181b was the most typically differentially expressed miRNA under hypoxic conditions. Functionally, these HRMs are involved in apoptosis, cell adhesion, cell proliferation and mRNA processing, all processes that associate closely with the hypoxia response of cancer cells. Additionally, we found that administration of miR-181b inhibitor can suppress proliferation of retinoblastoma cells. These findings provide the first evidence that miRNAs play an important role in the hypoxia response of retinoblastoma cells. MiR-181b, the most typically up-regulated miRNA may aid in future clinical intervention of

  12. Las Perspectivas y Retos de la Antropología Aplicada en el Siglo XXI

    Directory of Open Access Journals (Sweden)

    Marisol Pérez L.

    2007-01-01

    Full Text Available El presente artículo reflexiona acerca del rol de la Antropología aplicada en el siglo XXI. Analiza las posibilidades de la antropología aplicada en el contexto actual, sus fortalezas y debilidades frente a los problemas sociales contemporáneos.

  13. OTIMIZAÇÃO DO RENDIMENTO DO CONVERSOR DAB APLICADO AO TRANSFORMADOR ELETRÔNICO

    OpenAIRE

    Renan Piveta

    2015-01-01

    Este trabalho realiza uma investigação aprofundada sobre os diferentes padrões de modulação três níveis três níveis que o conversor DAB pode assumir, e o impacto que esses diferentes padrões provocam sobre a energia circulante na estrutura e sobre o rendimento. O conversor DAB pode ser controlado por três variáveis de projeto, aqui definidos como trio de controle (D1, D2, φ). D1 é a razão cíclica aplicada ao Full Bridge do lado de alta tensão, D2 a razão cíclica aplicada ...

  14. Intra-arterial chemotherapy for retinoblastoma: First Indian report

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    Pukhraj Rishi

    2015-01-01

    Full Text Available Aim: To describe treatment outcomes and complications of selective intra-arterial chemotherapy (IAC for retinoblastoma (RB in Indian eyes. Materials and Methods: Single center, retrospective interventional case series of 6 eyes with RB who underwent IAC using Melphalan (3 mg/5 mg/7.5 mg and topetecan (1 mg (n = 4 or melphalan (3 mg/5 mg/7.5 mg alone (n = 2 between December 2013 and June 2014. In all, 17 IAC procedures were performed using selective ophthalmic artery cannulation. Treatment outcomes were evaluated in terms of tumor control, vitreous and subretinal seeds control and globe salvage rates. Results: IAC was employed as primary (n = 1 or secondary (n = 5 modality of treatment. Each eye received mean 3 IAC sessions (median: 3; range: 1-4 sessions. Eyes were classified according to international classification of RB as Group B (n = 1, C (n = 1, D (n = 2 and E (n = 2. Following IAC, complete regression of the main tumor was seen in 3 cases (50%, partial regression in 2 (33%, while 1 case (15% showed no response. Of 4 eyes with subretinal seeds, 1 (25% eye had complete regression while 3 (75% eyes had partial regression. Of 5 eyes with vitreous seeds, 2 (40% eyes had complete regression while 3 (60% eyes had a partial response. Globe salvage was achieved in 5 of 6 eyes (83%. Diffuse choroidal atrophy and vitreous hemorrhage were observed in 1 (17% eye, each. No hematologic toxicity or cerebro-vascular events were observed. Mean follow-up period was 5.5 months (median: 6 months, range: 1-6 months. Conclusion: IAC is an effective therapy for globe preservation in eyes with RB. Larger studies with longer follow-up are required to validate these results.

  15. Quality of life of adult retinoblastoma survivors in the Netherlands

    Directory of Open Access Journals (Sweden)

    Cohen-Kettenis Peggy T

    2007-06-01

    Full Text Available Abstract Background To assess the quality of life (QoL and predictors thereof in Dutch adult hereditary and non-hereditary retinoblastoma (RB survivors. Methods In this population-based cross-sectional study, a generic QoL questionnaire (SF-36 and a disease-specific interview were administered to 87 adult RB survivors aged 18 to 35 years. Their QoL data were compared with those of a Dutch healthy reference group. Among the RB hereditary/non-hereditary survivors, the QoL was compared and predictors for QoL were identified by linear multiple regression analyses. Results As a group, RB survivors scored significantly lower than the reference group on the SF-36 subscale 'mental health' (t = -27, df = 86, p Conclusion In this exploratory study, it appears that the group of adult RB survivors experience a relatively good overall but slightly decreased QoL compared with the reference group. However, they report more problems with regard to their mental health (anxiety, feelings of depression, and loss of control. Hereditary RB survivors differ significantly from non-hereditary RB survivors only in 'general health'. Bullying in childhood and subjective experience of impairment are the main predictors of a worse QoL. In order to prevent worsening of QoL, or perhaps to improve it, clinicians should make an inventory of these issues at an early stage. We recommend further research to assess the specific psychological factors that may lead to mental health problems in this population.

  16. The Retinoblastoma pathway regulates stem cell proliferation in freshwater planarians.

    Science.gov (United States)

    Zhu, Shu Jun; Pearson, Bret J

    2013-01-15

    Freshwater planarians are flatworms of the Lophotrochozoan superphylum and are well known for their regenerative abilities, which rely on a large population of pluripotent adult stem cells. However, the mechanisms by which planarians maintain a precise population of adult stem cells while balancing proliferation and cell death, remain to be elucidated. Here we have identified, characterized, and functionally tested the core Retinoblastoma (Rb) pathway components in planarian adult stem cell biology. The Rb pathway is an ancient and conserved mechanism of proliferation control from plants to animals and is composed of three core components: an Rb protein, and a transcription factor heterodimer of E2F and DP proteins. Although the planarian genome contains all components of the Rb pathway, we found that they have undergone gene loss from the ancestral state, similar to other species in their phylum. The single Rb homolog (Smed-Rb) was highly expressed in planarian stem cells and was required for stem cell maintenance, similar to the Rb-homologs p107 and p130 in vertebrates. We show that planarians and their phylum have undergone the most severe reduction in E2F genes observed thus far, and the single remaining E2F was predicted to be a repressive-type E2F (Smed-E2F4-1). Knockdown of either Smed-E2F4-1 or its dimerization partner Dp (Smed-Dp) by RNAi resulted in temporary hyper-proliferation. Finally, we showed that known Rb-interacting genes in other systems, histone deacetylase 1 and cyclinD (Smed-HDAC1; Smed-cycD), were similar to Rb in expression and phenotypes when knocked down by RNAi, suggesting that these established interactions with Rb may also be conserved in planarians. Together, these results showed that planarians use the conserved components of the Rb tumor suppressor pathway to control proliferation and cell survival.

  17. Discharge and infection in retinoblastoma post-enucleation sockets

    Science.gov (United States)

    Mourits, Daphne L; Hartong, Dyonne T; Budding, Andries E; Bosscha, Machteld I; Tan, H Stevie; Moll, Annette C

    2017-01-01

    Purpose To investigate the causes and treatment options for socket discharge and infection in patients enucleated for retinoblastoma (Rb). Methods A questionnaire was filled out by (parents of) ocular prosthesis-wearing patients with a history of enucleation as treatment for Rb. We collected data on patients’ characteristics, cleaning habits of the prosthesis, frequency of socket irritation, discharge, and infection, and use of antibiotics. With ordinal logistic regression analysis, factors related to the outcome parameters (frequency of irritation, mucoid and purulent discharge) were identified. In a subset of young asymptomatic and symptomatic patients, a swab culture of the socket was performed to determine the presence of microorganisms. Results A total of 186 patients or their parents (mean age of the patients: 17.3 years, ranging from 0.8 to 88.3 years) filled out the questionnaire. Irritation, mucoid discharge, and purulent discharge were frequently (once a month or more often) experienced in 75 (39.5%), 127 (66.8%), and 15 (13.2%) sockets, respectively. Younger age was associated with a higher frequency of mucoid and purulent discharge. Radiation therapy, chemotherapy, gender, age at surgery, cleaning frequency, and nocturnal wear were not associated with the outcome parameters. In a subgroup of 26 patients, the sockets were swabbed and cultured. All symptomatic patients had a positive bacterial culture versus 15% (2/13) of the asymptomatic patients (Pprosthesis-wearing patients often experienced mucoid discharge, and less often irritation and socket infection. These complaints were found to decrease with increasing age, but did not seem to be influenced by cleaning or wearing habits. Symptomatic sockets, with and without discharge, were correlated with the presence of pathogenic bacteria for which local antibiotic treatment seemed effective in most cases. PMID:28280296

  18. Effects of PPARγ agonistrosiglitazone on human retinoblastoma cell in vitro and in vivo.

    Science.gov (United States)

    Cao, Xianyong; He, Lin; Li, Yanhua

    2015-01-01

    The aim of the study was to evaluate the antitumor effects of the PPARγ agonist rosiglitazone on the human retinoblastoma. The cell biological behavior was detected, specifically, the effects of rosiglitazone on cell viability and apoptosis of the human retinoblastoma Y79 cells were investigated by MTT assay and Hochest 33258 staining and the migration assay showed that rosiglitazone blocked the invasion and migration of the carcinoma cells through the reconstituted extracellular matrix (Matrigel). The effect of rosiglitazone on NF-κB-dependent reporter gene transcription induced by LPS was analyzed by NF-κB-luciferase assay. Then human retinoblastoma Y79 cells were subcutaneously transplanted in BALB/c nude mice, and the animals were treated with rosiglitazone (20 mg/kg, 40 mg/kg, and 80 mg/kg) to verify its anti-tumor effect in vivo. Rosiglitazone suppressed the viability of Y79 cells dose- and time-dependently and induced apoptosis in Y79 cells in vitro. Molecular biology analysis found that rosiglitazone could modulate the proliferative and apoptosis related signal, reduce NF-κB-dependent reporter gene transcription induced by LPS. Rosiglitazone markedly reduced the growth of Y79 cells transplanted into the mice without causing significant side effects. Our results suggested that rosiglitazone demonstrated antitumor activity against the human retinoblastoma Y79 cells by inhibiting cell growth, inducing apoptosis and inhibiting metastasis and invasion in vitro and delaying tumor growth in vivo.

  19. Crystal structure of human esterase D: a potential genetic marker of retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Dong; Li, Yang; Song, Gaojie; Zhang, David; Shaw, Neil; Liu, Zhi-Jie; (Chinese Aca. Sci.)

    2009-07-10

    Retinoblastoma (RB), a carcinoma of the retina, is caused by mutations in the long arm of chromosome 13, band 13q14. The esterase D (ESD) gene maps at a similar location as the RB gene locus and therefore serves as a potential marker for the prognosis of retinoblastoma. Because very little is known about the structure and function of ESD, we determined the 3-dimensional structure of the enzyme at 1.5 {angstrom} resolution using X-ray crystallography. ESD shows a single domain with an {alpha}/{beta}-hydrolase fold. A number of insertions are observed in the canonical {alpha}/{beta}-hydrolase fold. The active site is located in a positively charged, shallow cleft on the surface lined by a number of aromatic residues. Superimposition studies helped identify the typical catalytic triad residues -- Ser-153, His264, and Asp230 -- involved in catalysis. Mutagenesis of any of the catalytic triad residues to alanine abolished the enzyme activity. Backbone amides of Leu54 and Met150 are involved in the formation of the oxyanion hole. Interestingly, a M150A mutation increased the enzyme activity by 62%. The structure of human ESD determined in this study will aid the elucidation of the physiological role of the enzyme in the human body and will assist in the early diagnosis of retinoblastoma. Wu, D., Li, Y., Song, G., Zhang, D., Shaw, N., Liu, Z. J. Crystal structure of human esterase D: a potential genetic marker of retinoblastoma.

  20. RB1 mutation spectrum in a comprehensive nationwide cohort of retinoblastoma patients

    NARCIS (Netherlands)

    Dommering, Charlotte J.; Mol, Berber M.; Moll, Annette C.; Burton, Margaret; Cloos, Jacqueline; Dorsman, Josephine C.; Meijers-Heijboer, Hanne; van der Hout, Annemarie H.

    2014-01-01

    Background Retinoblastoma (Rb) is a childhood cancer of the retina, commonly initiated by biallelic inactivation of the RB1 gene. Knowledge of the presence of a heritable RB1 mutation can help in risk management and reproductive decision making. We report here on RB1 mutation scanning in a unique na

  1. Delayed mutation as a cause of retinoblastoma: application to genetic counseling.

    Science.gov (United States)

    Herrmann, J

    1976-01-01

    The genealogic and genetic data on retinoblastoma were reviewed and interpreted according to the model of delayed mutation; then applications of the model to specific situations in genetic counseling were considered. Patients with multiple congenital abnormalities and systemic chromosome aberrations are regarded as belonging to a different category of retinoblastoma cases than the more common patients without such abnormalities. The model of delayed mutation is considered for the latter group of patients. According to the model, mutation at the retinoblastoma locus can be delayed or complete and can occur during meiotic or mitotic cell division. Genotypically, three clases of individuals can be identified in retinoblastoma families: homozygous normal, heterozygous for the premutated allele, and heterozygous for the (fully) mutated allele; the other possible combinations of individuals have apparently not been observed. There is to date no evidence to suggest incomplete penetrance of the mutant allele, but 14% of individuals who have the mutant gene are "only" unilaterally affected. Carriers produce normal, affected and carrier offspring in the empiric proportion of, respectively, 54.5%, 36.4% and 9.1%. Most difficulties in genetic counseling arise because affected individuals may have inherited the premutated or the mutated allele and because unaffected individuals may have inherited the normal or the premutated allele. These aspects were considered for individuals presenting as sporadic-unilateral, sporadic-bilateral, familial-unilateral and familial-bilateral cases, and the empiric risk figures for various situations were quoted from the literature.

  2. Loss of the retinoblastoma protein-related p130 protein in small cell lung carcinoma

    DEFF Research Database (Denmark)

    Helin, K; Holm, K; Niebuhr, A;

    1997-01-01

    been described in a variety of human tumors, including retinoblastomas, osteosarcomas, and small cell lung carcinomas. Despite the structural and functional similarity between pRB, p107, and p130, alterations in the latter two proteins have not been identified in human tumors. We have screened a panel...

  3. A cancer derived mutation in the Retinoblastoma gene with a distinct defect for LXCXE dependent interactions

    Directory of Open Access Journals (Sweden)

    Demone Jordan

    2010-03-01

    Full Text Available Abstract Background The interaction between viral oncoproteins such as Simian virus 40 TAg, adenovirus E1A, and human papilloma virus E7, and the retinoblastoma protein (pRB occurs through a well characterized peptide sequence, LXCXE, on the viral protein and a well conserved groove in the pocket domain of pRB. Cellular proteins, such as histone deacetylases, also use this mechanism to interact with the retinoblastoma protein to repress transcription at cell cycle regulated genes. For these reasons this region of the pRB pocket domain is thought to play a critical role in growth suppression. Results In this study, we identify and characterize a tumor derived allele of the retinoblastoma gene (RB1 that possesses a discrete defect in its ability to interact with LXCXE motif containing proteins that compromises proliferative control. To assess the frequency of similar mutations in the RB1 gene in human cancer, we screened blood and tumor samples for similar alleles. We screened almost 700 samples and did not detect additional mutations, indicating that this class of mutation is rare. Conclusions Our work provides proof of principal that alleles encoding distinct, partial loss of function mutations in the retinoblastoma gene that specifically lose LXCXE dependent interactions, are found in human cancer.

  4. Growth suppression by p16ink4 requires functional retinoblastoma protein

    NARCIS (Netherlands)

    Medema, R.H.; Herrera, R.E.; Lam, F.; Weinberg, R.A.

    1995-01-01

    p16ink4 has been implicated as a tumor suppressor that is lost from a variety of human tumors and human cell lines. p16ink4 specifically binds and inhibits the cyclin-dependent kinases 4 and 6. In vitro, these kinases can phosphorylate the product of the retinoblastoma tumor suppressor gene. Thus, p

  5. Cell-nonautonomous function of the retinoblastoma tumour suppressor protein: new interpretations of old phenotypes.

    NARCIS (Netherlands)

    D. Whyatt (David); F.G. Grosveld (Frank)

    2002-01-01

    textabstractLoss of the retinoblastoma protein (pRb) induces a cell-nonautonomous defect in both erythroid and neuronal differentiation. It has previously been thought that this reflects a requirement for pRb function in cells that normally support erythropoiesis and neurogenesis, rather than in the

  6. Regulation of the retinoblastoma protein-related p107 by G1 cyclin complexes

    NARCIS (Netherlands)

    Beijersbergen, R.L.; Carlée, L.; Kerkhoven, R.M.; Bernards, R.A.

    1995-01-01

    The orderly progression through the cell cycle is mediated by the sequential activation of several cyclin/cyclin-dependent kinase (cdk) complexes. These kinases phosphorylate a number of cellular substrates, among which is the product of the retinoblastoma gene, pRb. Phosphorylation of pRb in late G

  7. Expression of FasL in Proliferation of Retinoblastoma Cells: A Mechanism Fas Counterattack

    Directory of Open Access Journals (Sweden)

    Hendrian Dwikoloso Soebagjo

    2014-08-01

    Full Text Available Purpose: The aim of this study were to determine the association of increasing of FasL with increasing proliferation of retinoblastoma cells. Materials and Methods: The protein expression was analyzed in 30 retinoblastoma samples from paraffin block using immunohistochemical method for evaluation of FasL, CDK4, and Ki-67 expression. Results: Among 30 retinoblastoma samples, FasL expression majority was negative in 33.3 % (10 samples and strong in 36,8 % (11 samples. CDK4 majority 53,3% was weak expression and Ki-67 was high expression also in 53,3% (16 samples. The expression of FasL was significantly related to CDK4 (r: 0.363; p: 0.048. The CDK4 was also significantly related to Ki-67 expression (r: 0.601; p: 0.000. Conclusion: The increasing of FasL on the mechanism Fas counterattack induces proliferation of retinoblastoma cells. [Cukurova Med J 2014; 39(4.000: 788-794

  8. Alteration of plant meristem function by manipulation of the Retinoblastoma-like plant RRB gene

    Science.gov (United States)

    Durfee, Tim; Feiler, Heidi; Gruissem, Wilhelm; Jenkins, Susan; Roe, Judith; Zambryski, Patricia

    2007-01-16

    This invention provides methods and compositions for altering the growth, organization, and differentiation of plant tissues. The invention is based on the discovery that, in plants, genetically altering the levels of Retinoblastoma-related gene (RRB) activity produces dramatic effects on the growth, proliferation, organization, and differentiation of plant meristem.

  9. The retinoblastoma-histone deacetylase 3 complex inhibits PPARgamma and adipocyte differentiation

    DEFF Research Database (Denmark)

    Fajas, Lluis; Egler, Viviane; Reiter, Raphael

    2002-01-01

    The retinoblastoma protein (RB) has previously been shown to facilitate adipocyte differentiation by inducing cell cycle arrest and enhancing the transactivation by the adipogenic CCAAT/enhancer binding proteins (C/EBP). We show here that the peroxisome proliferator-activated receptor gamma...

  10. Characterization of the retinoblastoma binding proteins RBP1 and RBP2

    DEFF Research Database (Denmark)

    Fattaey, A R; Helin, K; Dembski, M S;

    1993-01-01

    The retinoblastoma gene product, pRB, regulates cell proliferation by binding to and inhibiting the activity of key growth promoting proteins. Several cellular proteins have been shown to bind directly to pRB and the genes encoding a number of them have been isolated. The protein product of one...

  11. Perspectivas para a regulação das nanotecnologias aplicadas a alimentos e biocombustíveis

    Directory of Open Access Journals (Sweden)

    Wilson Engelmann

    2013-11-01

    Full Text Available O artigo aborda as possibilidades de regulação das nanotecnologias aplicadas aos alimentos e aos biocombustíveis. Nesse sentido, busca-se estudar os riscos e perigos advindos dessa junção, bem como algumas alternativas regulatórias, tomando-se como referência fórmulas alternativas não derivadas do Estado e do Poder Legislativo, mas oriundas de órgãos internacionais, das empresas envolvidas e de programas de cumprimento voluntário das regras e princípios, já vigentes, mas não diretamente relacionadas às nanotecnologias. Com isso, o estudo aponta possibilidades de o Direito ingressar nas perspectivas abertas pela Revolução Nanotecnológica, fomentando o cumprimento de normas que tenham como principal foco a saúde e a segurança do ser humano e a preservação do meio ambiente.

  12. Discharge and infection in retinoblastoma post-enucleation sockets

    Directory of Open Access Journals (Sweden)

    Mourits DL

    2017-03-01

    Full Text Available Daphne L Mourits,1 Dyonne T Hartong,1 Andries E Budding,2 Machteld I Bosscha,1 H Stevie Tan,1 Annette C Moll1 1Department of Ophthalmology, VU University Medical Center, 2Department of Microbiology, Academic Medical Center, Amsterdam, the Netherlands Purpose: To investigate the causes and treatment options for socket discharge and infection in patients enucleated for retinoblastoma (Rb. Methods: A questionnaire was filled out by (parents of ocular prosthesis-wearing patients with a history of enucleation as treatment for Rb. We collected data on patients’ characteristics, cleaning habits of the prosthesis, frequency of socket irritation, discharge, and infection, and use of antibiotics. With ordinal logistic regression analysis, factors related to the outcome parameters (frequency of irritation, mucoid and purulent discharge were identified. In a subset of young asymptomatic and symptomatic patients, a swab culture of the socket was performed to determine the presence of microorganisms. Results: A total of 186 patients or their parents (mean age of the patients: 17.3 years, ranging from 0.8 to 88.3 years filled out the questionnaire. Irritation, mucoid discharge, and purulent discharge were frequently (once a month or more often experienced in 75 (39.5%, 127 (66.8%, and 15 (13.2% sockets, respectively. Younger age was associated with a higher frequency of mucoid and purulent discharge. Radiation therapy, chemotherapy, gender, age at surgery, cleaning frequency, and nocturnal wear were not associated with the outcome parameters. In a subgroup of 26 patients, the sockets were swabbed and cultured. All symptomatic patients had a positive bacterial culture versus 15% (2/13 of the asymptomatic patients (P<0.001. Common cold was correlated with both symptoms and presence of bacteria. Haemophilus influenzae and Staphylococcus aureus were the species most frequently cultured. Conclusion: Ocular prosthesis-wearing patients often experienced mucoid

  13. Infrared thermography applied to monitoring of radioactive waste drums; Termografia infravermelha aplicada ao monitoramento de tambores de rejeitos radioativos

    Energy Technology Data Exchange (ETDEWEB)

    Kelmer, P.; Camarano, D.M. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Calado, F.; Phillip, B.; Viana, C.; Andrade, R.M., E-mail: paulafuziki@yahoo.com.br, E-mail: flavio.arcalado@gmail.com, E-mail: bruno.phil@gmail.com, E-mail: criisviana@hotmail.com, E-mail: rma@ufmg.br, E-mail: dmc@cdtn.br [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Departamento de Engenharia Eletrica

    2013-07-01

    The use of thermography in the inspection of drums containing radioactive waste is being stimulated by the absence of physical contact. In Brazil the majority of radioactive wastes are compacted solids packed in metal drums stored temporarily for decades and requires special attention. These drums have only one qualitative indication of the radionuclides present. However, its structural condition is not followed systematically. The aim of this work is presents a methodology by applying thermography for monitoring the structural condition of drums containing radioactive waste in order to detect degraded regions of the drums. (author)

  14. Otimização aplicada ao problema de alocação de equipes em uma panificadora

    Directory of Open Access Journals (Sweden)

    Juliana Triches

    2015-01-01

    Full Text Available To allocate a team is to assign the responsibilities for a task to a group of human resources. Developers may have different technical capabilities, and the tasks may have different needs. Due to the complexity of solving this kind of problem, the ideal solution requires the application of optimization techniques. Thus, the aim of this study was to provide a tool to support and guide decisions in the allocation of teams at a bakery, seeking to minimize idle time. The mathematical resolution of the problem is given through the formulation of a linear optimization model, using as a basis the resolution of the Transport Problem. This was accomplished using Lingo, a software program suitable for solving this type of problem. It was observed that formulating and solving the case as an optimization problem led to a more uniform distribution of activities, respecting employees´ skills and avoiding the concentration of tasks on a single individual.

  15. Biotecnologia aplicada ao melhoramento genético do cafeeiro Biotechnology applied to the genetic improvement of coffee plant

    Directory of Open Access Journals (Sweden)

    Tâmara Prado de Morais

    2011-05-01

    Full Text Available O melhoramento genético do cafeeiro mediante técnicas convencionais é trabalhoso e demorado. A biotecnologia oferece estratégias alternativas para auxiliar na multiplicação e no desenvolvimento de novas variedades com resistência a estresses bióticos e abióticos, melhor qualidade de bebida e maturação mais uniforme dos frutos. As técnicas de cultura de tecidos têm possibilitado a obtenção de grande número de plantas e a garantia da uniformidade genética do material. O emprego de marcadores moleculares, principalmente através da seleção assistida, facilitou o rápido progresso do melhoramento genético da cultura, assim como a transformação genética, via cultura e fusão de protoplastos, biobalística ou mediada por Agrobacterium sp. Esta revisão objetiva sumarizar o histórico, situação atual e perspectivas da biotecnologia no melhoramento genético do cafeeiro.Genetic improvement of coffee through classical breeding is laborious and time consuming. Biotechnology offers alternative strategies to assist multiplication and development of new and improved coffee varieties, including those resistant to biotic and abiotic stresses, with better cup quality, and with uniform fruit maturation. Tissue culture techniques have enabled the production of a large number of plants with genetic uniformity. The use of molecular markers, especially through assisted selection, led to rapid progress of coffee plant breeding, as well as the use of genetic transformation by protoplasts culture and fusion, biobalistics, or Agrobacterium-mediated. This review provides a summary of biotechnology history, current situation and directions applied to the genetic improvement of coffee plant.

  16. EPIDEMIOLOGIA MOLECULAR APLICADA AO MONITORAMENTO DE ESTIRPES DE Staphylococcus aureus NA PRODUÇÃO DE QUEIJO MINAS FRESCAL

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    Maria Izabel Merino de Medeiros

    2013-03-01

    Full Text Available We studied the molecular epidemiology of Staphylococcus aureus strains potentially toxigenic, isolated from the production process of Minas frescal cheese in a small dairy plant in the state of São Paulo. For this, samples were taken during the period from June 2008 to July 2009. Samples were collected from the surface of the receiving and storage tanks of raw milk, the surface of the balance tank of pasteurized milk, the water supply system, the pipes and equipments, the hands of the handler and from the packaged cheese, totaling 140 samples. The colonies isolated on Baird-Parker Agar confirmed as Gram positive and positive for catalase, coagulase and acetoin production, were submitted to extraction of bacterial DNA using the Invitek - Uniscience® kit. Confirmation of the isolated species and enterotoxins SEA, SEB, SEC, SED and TSST-1 toxin was carried out through the amplification of specific fragments of chromosomal DNA. Among the 74 strains of isolated coagulase-positive staphylococci, only 41 (55.4% strains were confirmed as Staphylococcus aureus, of which 25 (61.0% were positive to the presence of staphylococcal toxins. The most frequently identified enterotoxin was SEA. The toxigenic strains of Staphylococcus aureus were more frequently isolated from hands of the handler (16.0%, raw milk receiving tank (12.0%, pasteurized milk for cheese making (12.0% and fresh white cheese ready for consumption (12.0%.

  17. Inferências pedológicas aplicadas ao perímetro irrigado de Custódia, PE

    Directory of Open Access Journals (Sweden)

    Oliveira Lindomário Barros de

    2002-01-01

    Full Text Available O objetivo deste trabalho foi avaliar as propriedades e o comportamento de solos aluviais do perímetro irrigado de Custódia, PE, cujo uso agrícola foi interrompido em virtude de problemas de salinidade/sodicidade. Foram realizadas caracterizações morfológica, física, química e mineralógica de três perfis de solos afetados por sais do referido perímetro irrigado. Os solos foram classificados, de acordo com o Sistema Brasileiro de Classificação de Solos, como Neossolo Flúvico Ta eutrófico solódico, Neossolo Flúvico Sódico salino e Neossolo Flúvico Sódico típico. A estratificação, típica de solos de natureza aluvial, rege o comportamento físico e químico dos solos estudados no sentido vertical. Apesar da presença de minerais que podem contribuir com o processo de salinização/sodificação, tal contribuição deve ser irrisória quando comparada à das águas de rega de baixa qualidade. A reutilização destes solos com agricultura irrigada deve ser precedida do uso de corretivos e da introdução de um eficiente sistema de drenagem que impeça a ascensão do lençol freático e promova a lixiviação do sódio.

  18. Cokrigagem colocada aplicada ao mapeamento do nível de água subterrânea

    OpenAIRE

    Maria Noel Morales Boezio; João Felipe C. L. Costa; Koppe,Jair C.

    2006-01-01

    A mineração subterrânea pode afetar o ciclo hidrológico, especialmente a circulação da água de infiltração de uma dada região. Em particular, o nível de água pode ser modificado, refletindo-se no meio ambiente circundante. Em programas de monitoramento de água subterrânea, o mapeamento do nível de água é parte fundamental. O mesmo ajuda na determinação da direção de fluxo da água subterrânea, identificação de zonas de recarga e avaliação da dispersão de contaminantes. A posição do nível de ág...

  19. A biotecnologia aplicada ao melhoramento genético vegetal: controvérsias e discussões

    Directory of Open Access Journals (Sweden)

    Andréia Morais Xavier de OLIVEIRA

    2012-01-01

    Full Text Available This issue concerns plant breeding, with biotechnology at its beginning and progress, if it is seen as the "newgreen revolution," emphasizing the brazilian agricultural biotechnology and its development related to the leadership ofthe Brazilian Agricultural Research (EMBRAPA, considering the possible risks to both the environment and humanhealth, respecting and practicing the laws and rules of biosafety. Also reports issues of Microbial Genetics,Microbiology and Molecular Biology in order to clarify various techniques of gene cloning, ranging from the choice offeatures aimed at improving a particular product by the process of isolation and transformation of these genes, using themost modern technologies.

  20. Análise de minerais pesados aplicada ao mapeamento geológico na Bacia Paraíba

    OpenAIRE

    Felipe Lamus Ochoa

    2010-01-01

    O mapeamento geológico dos depósitos siliciclásticos aflorantes nas bacias marginais do nordeste brasileiro tem gerado um alto volume de designações estratigráficas ainda abertas às discussões. No intuito de subsidiar a resolução deste problema cartográfico foi escolhida a parte emersa da Bacia Paraíba, mais especificamente as sub-bacias Alhandra e Mirirí. Para isto foi realizada a caracterização mineralógica e petrográfica dos arenitos cenozóicos da Bacia Paraíba, com vistas a identificação ...

  1. Technologies applied to wells producing gas in Bolivia; Tecnologias aplicadas aos pocos produtores de gas em Bolivia

    Energy Technology Data Exchange (ETDEWEB)

    Nobrega, Fernando R.B. [Petroleo Brasileiro S.A. (PETROBRAS), Rio de Janeiro, RJ (Brazil); Carrillo, Marco A.; Barrerro, Dennys A. [PETROBRAS Bolivia S.A., La Paz (Bolivia)

    2012-07-01

    The This paper seeks to highlight the engineering, lessons learned and topics for improvement of the technologies used in gas wells drilled between November 2008 and October 2011 in an environment of Bolivian' subandino. Among technologies employed and commented hereunder has the drilling gas reservoirs in near balance using a dual phase fluid, with nitrogen; carrying out multilateral wells equipped with intelligent completion in such environment; as well as other technologies presented herein. This document was prepared on drilling operations performed in SAL-15, SAL-17, SBLSBL- 7 and SBL-8, comprising the period from November 2008 to October 2011. (author)

  2. Gestão Multidimensional da Informação Aplicada ao Desenvolvimento Turístico

    Directory of Open Access Journals (Sweden)

    Adolfo Alberto Vanti

    2003-05-01

    Full Text Available Iniciativas empresariais e acadêmicas no contexto do setor de turismo são verificadas diariamente no sentido de tornar este setor cada vez mais estratégico para a economia brasileira. Estas iniciativas auxiliam no aprimoramento dos negócios, tornando-os cada vez mais científicos e profissionalizados. O presente trabalho colabora com esta profissionalização através do desenvolvimento de ações sustentadas na gestão da informação. Os consumidores buscam produtos e serviços cada vez mais customizadas (adaptados às suas necessidades em um mercado de alta complexidade. Porém, os empresários do setor de turismo necessitam adaptar esta demanda para uma customização em massa (mass customization através do desenvolvimento de estratégias baseadas na informação. Este trabalho propõe a utilização da técnica de gestão multidimensional da informação chamada Business Intelligence (BI para identificar algumas tendências de mercado, as quais posteriormente podem ser exploradas por pequenos empreendedores do setor turístico.

  3. MaTUTO: adaptação da metodologia de aprendizagem baseada em problemas aplicada ao ensino de ontologias

    Directory of Open Access Journals (Sweden)

    Ricardo Alexandre Afonso

    2013-08-01

    Full Text Available Introduction: The introduction of new teaching methodologies brings a proposal for the evolution of traditional teaching models, which have been shown to be less efficient due to the evolution of society and the use of technology. This inadequacy educational encouraged the construction of a proposal that condenses the steps of the Problem-Based Learning methodology, which was tested in the creation of a domain ontology defined by a tutor responsible for the teaching/learning activity. The article aims to present a methodology compatible with a dynamic reality that takes place at information systems disciplines and classes. The students involved in the experiment were also invited to evaluate the experience. Method: It presents the main features and steps of the Problem-Based Learning methodology, adjusted in the proposed MaTUTO (Map, TUtoring and Translate in Ontology, which was applied to undergraduate students. Results: It was possible to apply a methodology that adapts and condenses the original steps of the Problem-Based Learning approach as a support to subjects taught at Information Systems courses. The ontology was built and found satisfactory, as well as the results, coming from the students’ evaluation, were encouraging. Conclusion: Concerning the theme - the domain ontology - the alternative methodological proposal allowed the students not only to grasp the concepts involved in the Problem-Based Learning approach, but also had an information product outcome: the ontology itself.

  4. Simulação Monte Carlo com Repesagem Aplicada ao Calor Específico de Sólidos

    Directory of Open Access Journals (Sweden)

    Silva Roberto da

    2002-01-01

    Full Text Available Apresenta-se uma estimativa numérica para a contribuição das vibrações da rede para o calor específico de um sólido cristalino. O método Monte Carlo é utilizado tanto em sua forma original quanto em sua versão mais moderna, que emprega a técnica da repesagem para extrair mais informações de cada simulação. Mostra-se como obter curvas contínuas para grandezas como a energia e o calor específico em função da temperatura a partir de simulação feita em apenas uma temperatura. Os resultados analíticos são incluídos para facilitar a comparação com os resultados da simulação.

  5. AO Group Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Olivier, S

    2005-10-04

    The Adaptive Optics (AO) Group in I Division develops and tests a broad range of advanced wavefront control technologies. Current applications focus on: Remote sensing, High power lasers, Astronomy, and Human vision. In the area of remote sensing, the AO Group leads a collaborative effort with LLNL's Nonproliferation, Arms Control & International Security (NAI) Directorate on Enhanced Surveillance Imaging. The ability to detect and identify individual people or vehicles from long-range is an important requirement for proliferation detection and homeland security. High-resolution imaging along horizontal paths through the atmosphere is limited by turbulence, which blurs and distorts the image. For ranges over {approx}one km, visible image resolution can be reduced by over an order of magnitude. We have developed an approach based on speckle imaging that can correct the turbulence-induced blurring and provide high resolution imagery. The system records a series of short exposure images which freeze the atmospheric effects. We can then estimate the image magnitude and phase using a bispectral estimation algorithm which cancels the atmospheric effects while maintaining object information at the diffraction limit of the imaging system.

  6. Hypoxia-induced miR-181b enhances angiogenesis of retinoblastoma cells by targeting PDCD10 and GATA6.

    Science.gov (United States)

    Xu, Xiaofang; Ge, Shengfang; Jia, Renbing; Zhou, Yixiong; Song, Xin; Zhang, He; Fan, Xianqun

    2015-06-01

    Previous findings showed that miR-181b is upregulated under hypoxic conditions in retinoblastoma cells. Since hypoxia is a common feature of retinoblastoma that affects tumor progression as well as tumor therapy, in the present study, we investigated the regulatory mechanism of miR-181b under hypoxic conditions, and examined the role of miR-181b in retinoblastoma responses to hypoxia (chemoresistance and angiogenesis) and possible downstream genes. The level of hypoxia-inducible factor-1α (HIF-1α) and miR-181b was detected to examine the link between them. Tube formation and cell cytotoxicity assays were used to clarify the effects of miR-181b on hypoxic responses of retinoblastoma cells. Bioinformatics analysis was performed to predict potential targets of miR-181b and western blotting was used to verify these targets. The results showed a significantly increased expression of HIF-1α in hypoxia-treated retinoblastoma cells. Downregulation of HIF-1α using a small-interfering RNA (siRNA) knockdown technology did not decrease the expression of miR-181b. Through gain- and loss-of-function studies, miR-181b was demonstrated to significantly stimulate the ability of capillary tube formation of endothelial cells. Programmed cell death-10 (PDCD10) and GATA binding protein 6 (GATA6) were identified as the target genes of miR‑181b. To the best of our knowledge, results of the present study provide the first evidence that miR-181b was upregulated by hypoxia in retinoblastoma in an HIF-1α-independent manner. miR-181b increased tumor angiogenesis of retinoblastoma cells. Additionally, miR-181b exerts its angiogenic function, at least in part, by inhibiting PDCD10 and GATA6. Thus, it is a new potentially useful therapeutic target for retinoblastoma.

  7. Antitumor activity of celastrol nanoparticles in a xenograft retinoblastoma tumor model

    Directory of Open Access Journals (Sweden)

    Li ZR

    2012-05-01

    Full Text Available Zhanrong Li,1,* Xianghua Wu,1,* Jingguo Li,2 Lin Yao,1 Limei Sun,1 Yingying Shi,1 Wenxin Zhang,1 Jianxian Lin,1 Dan Liang,1 Yongping Li1 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, 2School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, People's Republic of China*These authors contributed equally to this workBackground: Celastrol, a Chinese herbal medicine, has shown antitumor activity against various tumor cell lines. However, the effect of celastrol on retinoblastoma has not yet been analyzed. Additionally, the poor water solubility of celastrol restricts further therapeutic applications. The goal of this study was to evaluate the effect of celastrol nanoparticles (CNPs on retinoblastoma and to investigate the potential mechanisms involved.Methods: Celastrol-loaded poly(ethylene glycol-block-poly(ε-caprolactone nanopolymeric micelles were developed to improve the hydrophilicity of celastrol. The 2-(2-methoxy-4-nitrophenyl-3-(4-nitrophenyl-5-(2,4-disulf-ophenyl-2H tetrazolium monosodium salt (WST-8 assay was used to determine the inhibitory effect of CNPs on SO-Rb 50 cell proliferation in vitro. Immunofluorescence was used to evaluate the apoptotic effect of CNPs on nuclear morphology, and flow cytometry was used to quantify cellular apoptosis. The expression of Bcl-2, Bax, NF-κB p65, and phospo-NF-κB p65 proteins was assessed by Western blotting. A human retinoblastoma xenograft model was used to evaluate the inhibitory effects of CNPs on retinoblastoma in NOD-SCID mice. Hematoxylin and eosin staining was used to assess the apoptotic effects of CNPs on retinoblastoma.Results: CNPs inhibit the proliferation of SO-Rb 50 cells in a dose- and time-dependent manner with an IC50 of 17.733 µg/mL (celastrol-loading content: 7.36% after exposure to CNPs for 48 hours. CNPs induce apoptosis in SO-Rb 50 cells in a dose-dependent manner. The expression of Bcl-2, NF-κB p65, and phospo-NF-κB p65

  8. Long non-coding RNA HOTAIR regulates proliferation and invasion via activating Notch signalling pathway in retinoblastoma

    Indian Academy of Sciences (India)

    CHANGXIA DONG; SHAOYI LIU; YONGBIN LV; CHUNPING ZHANG; HEYING GAO; LIXIA TAN; HONG WANG

    2016-12-01

    Retinoblastoma is the most frequently occurring tumour in the eyes in early childhood. Novel targets that areimportant for the diagnosis or treatment of retinoblastoma could be valuable in increasing the survival rate of patientsaffected by this disease. Long non-coding RNAs (lncRNAs) are a recently discovered type of RNAs with no proteincodingfunction; yet it has become increasingly clear that lncRNAs are responsible for important gene regulatoryfunctions in various diseases. In this study, the expression of lncRNA HOTAIR was measured by qRT-PCR, andHOTAIR expression was found to be significantly upregulated in human retinoblastomas tissues as compared with thatin paracancerous tissues. Knockdown of HOTAIR restricted the proliferation and invasion of the more invasiveretinoblastoma Y79 cells, and led to G0/G1 arrest, possibly through inhibiting phospho-RB1, RB1 and CCNE.Furthermore, we found that the Notch signalling pathway was activated abnormally in retinoblastoma cell lines, whileknockdown of HOTAIR attenuated the endogenous Notch signalling pathway in vitro and in vivo. In addition,knockdown of HOTAIR could inhibit the tumour progression in a xenograft model of retinoblastoma. In summary,our findings indicate that HOTAIR may play important roles in retinoblastoma progression via Notch pathway.HOTAIR has the potential to enhance the development of novel targeted diagnostic and therapeutic approaches forretinoblastoma.

  9. Carta ao Leitor

    Directory of Open Access Journals (Sweden)

    Equipe Editorial RMCEAD

    2015-06-01

    Full Text Available Aos Leitores Caríssimos leitores, Com grande entusiasmo, apresentamos a mais nova edição da Revista Multitexto do CEAD/Unimontes. Esta edição conta com 12 novos trabalhos que, sob um prisma transdisciplinar de reflexões, tecem suas leituras e arranjos metodológicos incidindo sobre as ciências e a realidade, seu teor e proposta. Trata-se de artigos originais e de revisão que promovem um diálogo aberto com o mundo contemporâneo. O estado da arte de diversas linhas temáticas foram selecionados para que a comunidade científica possa celebrar o conhecimento dispensado nas páginas materiais e virtuais desse periódico em crescimento. Em seu terceiro ano de experiências em coligir e abrigar estudos de recorte interdisciplinar, com enfoque especial ao rol de temáticas da Educação a Distância, a Revista Multitexto adquire mais entusiasmo. Entusiasmo para se debruçar sobre textos que se expandem sobre o cotidiano e integram os caminhos acadêmicos aos caminhos da vida. Você será capaz de imergir em pesquisas e perspectivas que celebram o conhecimento como ferramenta de transformação da realidade. E, como costumeiro deste periódico, equipe editorial e instituição, trata-se da celebração de uma paixão inveterada pelo conhecimento. A jornada deste periódico caminha para a maturação de seus autos e de motivações originárias. É cada vez mais seguro o ensejo pela pesquisa nesta instituição de salvaguarda e é cada vez mais expressivo o esforço conjunto para que o conhecimento através de múltiplos textos – como propõe a denominação deste periódico – transpasse a realidade com frutos e benefícios amplos. Aprecie as colaborações da presente edição e junte-se neste tráfego do conhecimento, no qual o mundo hoje se move. Com grande estima, Equipe Editorial

  10. Ciencia básica y ciencia aplicada

    Directory of Open Access Journals (Sweden)

    Pérez-Tamayo Ruy

    2001-01-01

    Full Text Available En el contexto de una intervención en el Foro de Consulta Democrática de la Coordinación de los Institutos Nacionales de Salud sobre Enseñanza e Investigación en Salud, realizado en el Instituto Nacional de Cardiología Ignacio Chávez, se hace una revisión crítica de la clasificación convencional de la ciencia en básica y aplicada; además, se analiza lo que deberían ser la enseñanza y la investigación en salud. Para apoyar su propia clasificación de la ciencia en "bien hecha", la cual "genera conocimientos verificables sobre la realidad" y la "mal hecha", improductiva o productora de "puras mentiras" y "no debe patrocinarse", el autor revisa las definiciones utilitaristas y peyorativas como las que establecen ciencia comprometida y ciencia pura, ciencia útil y ciencia inútil, y ciencia práctica y ciencia esotérica, como sinónimos de ciencia aplicada y ciencia básica y afirma que, en México, esta diferenciación "para lo único que ha servido en el pasado es para justificar la reducción en el apoyo oficial a la ciencia básica, porque no estaba dirigida a resolver ´los problemas nacionales´o porque no caía en las prioridades establecidas en ese sexenio". En cuanto a educación e investigación en salud reconoce que el programa actual de formación de investigadores tiene una eficiencia muy baja y propone un estudio científico crítico, realizado por un equipo de especialistas, interdisciplinario, "para integrar la carrera del investigador científico desde la captura de la juventud inteligente hasta la jubilación o muerte del investigador" y para el cual la evaluación de la eficiencia del apoyo a sus proyectos de investigación no se restrinja a si se publicó o no un artículo "pues la calidad del trabajo científico y la contribución de un investigador al desarrollo de la ciencia no es nada más el número de sus publicaciones". El texto completo en inglés de este artículo está disponible en: http://www.insp.mx/salud/index.html

  11. Sistema experto de ergonomía aplicada

    Directory of Open Access Journals (Sweden)

    Jesús Mora Ruiz

    2004-01-01

    Full Text Available Son muchos los países que están utilizando las modernas tecnologías de la información aplicadas a la educación. Este fenómeno es parte de la nueva revolución industrial o era de la información que está afectando a casi todas las actividades humanas. La investigación, diseño e implementación de tecnologías que suplan las carencias de recursos económicos para la adquisición de laboratorios que cuenten con todo lo necesario para la realización de prácticas que reafirmen lo teórico. En el desarrollo del proceso de enseñanza-aprendizaje, debe tenerse en cuenta un modelo pedagógico, donde participen activamente tanto el catedrático como los estudiantes. El desarrollo de Sistemas Expertos, basados en Inteligencia Artificial, para realizar prácticas aplicadas a la Ingeniería Industrial, ofrece una posibilidad diferente de modelo educativo, con la que el alumno reafirmará una y otra vez la práctica, en la que aplicará los conocimientos teóricos adquiridos en las aulas; lo que dará un valor agregado a su profesión, para lograr un mejor desempeño en aplicaciones en áreas de la Ingeniería Industrial. Los Sistemas Expertos basados en la Inteligencia Artificial utilizados como emuladores basados en el conocimiento, diseñados y desarrollados por las propias instituciones con recursos propios, son una solución factible y económica, que resuelven las necesidades fundamentales en el proceso de enseñanza- aprendizaje; ya que con ellos se efectúan prácticas de diseños ergonómicos, al mismo tiempo que dan respuestas expertas para la aplicación de la materia de Ergonomía; factor imprescindible en la formación del Ingeniero Industrial

  12. PSICOLOGIA APLICADA À ODONTOPEDIATRIA: UMA INTRODUÇÃO

    Directory of Open Access Journals (Sweden)

    Áderson Luiz Costa Junior

    2013-10-01

    Full Text Available Apresenta-se, aqui, a psicologia aplicada à odontologia como um corpo de conhecimentos da psicologia clínica da saúde útil à avaliação e manipulação do repertório de comportamentos de indivíduos, especialmente crianças, expostos a tratamento odontológico, cuja situação é percebida como ameaçadora e/ou potencialmente aversiva. Discutem-se a aquisição e a manutenção de respostas de medo de dentista em crianças e as variáveis psicossociais que medeiam os processos de diagnóstico, tratamento e reabilitação em odontopediatria. Apresentam-se alguns procedimentos psicológicos de manipulação de repertório de comportamentos de pacientes aplicados à situação de consultório de odontologia. Pretende-se, assim, estimular a formação acadêmica e profissional do psicólogo para atuação em contextos de odontologia e de saúde de modo geral.

  13. Estrategias de financiamiento aplicadas por hipermercados en un entorno competitivo

    Directory of Open Access Journals (Sweden)

    Miraidy Leal Morantes

    2003-01-01

    Full Text Available El presente estudio tiene como objetivo analizar las estrategias de financiamiento aplicadas en el sector de la distribución de bienes de consumo masivo, tomando como referencia la experiencia de los hipermercados ubicados en Maracaibo, Estado Zulia. Se desarrolló una investigación de carácter descriptivo; para la recolección de datos se aplicó un cuestionario estructurado a gerentes generales y financieros, así como entrevistas no estructuradas a personal administrativo clave de las organizaciones. Los resultados evidencian que las estrategias de financiamiento utilizadas son la integración vertical en redes de proveedores, el endeudamiento bancario y la reinversión de utilidades, como manera de responder al nuevo escenario que plantea la economía mundial. Se concluye que la creciente penetración de las grandes cadenas en el país produce efectos que inciden directamente en proveedores, consumidores y empresas venezolanas del sector.

  14. Retinoblastoma tumor suppressor functions shared by stem cell and cancer cell strategies

    Institute of Scientific and Technical Information of China (English)

    Susumu; Kohno; Shunsuke; Kitajima; Nobunari; Sasaki; Chiaki; Takahashi

    2016-01-01

    Carcinogenic transformation of somatic cells resembles nuclear reprogramming toward the generation of pluripotent stem cells.These events share eternal escape from cellular senescence,continuous self-renewal in limited but certain population of cells,and refractoriness to terminal differentiation while maintaining the potential to differentiate into cells of one or multiple lineages.As represented by several oncogenes those appeared to be first keys to pluripotency,carcinogenesis and nuclear reprogramming seem to share a number of core mechanisms.The retinoblastoma tumor suppressor product retinoblastoma(RB)seems to be critically involved in both events in highly complicated manners.However,disentangling such complicated interactions has enabled us to better understand how stem cell strategies are shared by cancer cells.This review covers recent findings on RB functions related to stem cells and stem cell-like behaviors of cancer cells.

  15. Retinoblastoma protein functions as a molecular switch determining white versus brown adipocyte differentiation

    DEFF Research Database (Denmark)

    Hansen, Jacob B; Jørgensen, Claus; Petersen, Rasmus K;

    2004-01-01

    AMP sensitivity. Suppression of cAMP-dependent protein kinase activity in Rb(-/-)MEFs blocked the brown adipocyte-like gene expression pattern without affecting differentiation per se. Immunohistochemical studies revealed that pRB is present in the nuclei of white but not brown adipocyte precursor cells......Adipocyte precursor cells give raise to two major cell populations with different physiological roles: white and brown adipocytes. Here we demonstrate that the retinoblastoma protein (pRB) regulates white vs. brown adipocyte differentiation. Functional inactivation of pRB in wild-type mouse embryo...... fibroblasts (MEFs) and white preadipocytes by expression of simian virus 40 large T antigen results in the expression of the brown fat-specific uncoupling protein 1 (UCP-1) in the adipose state. Retinoblastoma gene-deficient (Rb-/-) MEFs and stem cells, but not the corresponding wild-type cells, differentiate...

  16. Immunohistochemical observation of anterior subcapsular cataract in eye with spontaneously regressed retinoblastoma.

    Science.gov (United States)

    Shirai, Kumi; Okada, Yuka; Saika, Shizuya

    2010-03-01

    We report the histological findings of secondary cataract in an eye with a spontaneously regressed retinoblastoma to obtain keys to clarify the mechanism of this phenomenon. During phacoemulsification, opacified anterior capsule was obtained, fixed in formalin, and embedded in paraffin. Paraffin sections of the specimen were histologically observed. Hematoxylin-eosin staining showed extracellular matrix accumulation in the extracted fibrous anterior subcapsular opacification. Immunohistochemistry revealed the presence of fibrous collagen types and cellular fibronectin. Presumed lens cells amid matrix were positively labeled for vimentin, alpha-smooth muscle actin, and phospho-Smad2. Histology of the fibrous anterior subcapsular opacification tissue showed the possibility of epithelial-mesenchymal transition of the lens epithelial cells in the secondary cataract following a spontaneously regressed retinoblastoma.

  17. Increased incidence and disparity of diagnosis of retinoblastoma patients in Guatemala.

    Science.gov (United States)

    Dean, Michael; Bendfeldt, Giovana; Lou, Hong; Giron, Veronica; Garrido, Claudia; Valverde, Patricia; Barnoya, Margarita; Castellanos, Mauricio; Jiménez-Morales, Silvia; Luna-Fineman, Sandra

    2014-08-28

    Analysis of 327 consecutive cases at a pediatric referral hospital of Guatemala reveals that retinoblastoma accounts for 9.4% of all cancers and the estimated incidence is 7.0 cases/million children, higher than the United States or Europe. The number of familial cases is low, and there is a striking disparity in indigenous children due to late diagnosis, advanced disease, rapid progression and elevated mortality. Nine germline mutations in 18 patients were found; two known and five new mutations. Hypermethylation of RB1 was identified in 13% of the tumors. An early diagnosis program could identify cases at an earlier age and improve outcome of retinoblastoma in this diverse population.

  18. A palliative approach for rehabilitation of a pediatric patient with retinoblastoma

    Directory of Open Access Journals (Sweden)

    Himanshi Aggarwal

    2013-01-01

    Full Text Available Retinoblastoma is a highly malignant neoplasm. Most of the cases are usually far advanced at the time of detection, requiring enucleation to salvage the child′s life. However, the cosmetic rehabilitation of these patients should always be an integral part of their treatment, which helps in their re-integration in the society. This paper presents a case of 5-year-old patient who had undergone enucleation of her left eye due to retinoblastoma. A multidisciplinary approach, including ophthalmologist, psychotherapist, and a prosthodontist, was adopted to meet her physical, psychological, functional, emotional, social, and cosmetic demands. The patient was rehabilitated cosmetically with precisely fabricated ocular prosthesis. An ocular prosthesis is a highly positive and non-invasive approach to improve the cosmetic appearance and psychological well-being of patient.

  19. O combate ao escorpionismo

    Directory of Open Access Journals (Sweden)

    Octavio de Magalhães

    1946-09-01

    Full Text Available O outor estuda os processos de combate aos acidentes pelas picadas dos escorpiões, visando: a Profilaxia e b Terapêutica. Refere-se à luta nos campos e nas cidades e aqui, dentro e fora dos domicílios. Assinala os diferentes métodos para o combate a êsses artrópodos peçonhentos. Fala na luta direta, indireta, na vacinação pelo anaveneno, na propaganda pela educação racional da população contra o perigo dos acidentes. Refere-se à "cata" dos escorpiões, à luta química, ao emprêgo de animais escorpiófagos, á feitura de casas e jardins anti-escorpiões. Na primeira parte do presente trabalho o autor trata da luta química pelo d. D. T. contra os tityus bahiensis e serrulatus, concluindo que êste corpo químico é um poderoso elemento de luta contra êstes escorpiões. Na segunda parte do trabalho, o autor trata da terapêutica dos acidentes. Mostra a necessidade do emprêgo convenientemente da única terapêutica racional e eficaz contra a intoxicação escorpiônica: a soroterapia específica. Assinala a necessidade da injeção de doses maciças de um sôro de alta valência, preparado em bovídeos, para evitar o mais possível o choque anafilático, no menor tempo possível após as picadas. Aconselha o empêgo de anaveneno escorpiônico, para vacinação principalmente de crianças de baixa idade, nos lugares fortemente infestados pelos escorpiões (principalmente Tityus serrulatus, maximé quando no local não houver sôro anti-escorpiônico específico, como meio preventivo contra a gravidade das intoxicações. Cita finalmente os trabalhos recentes de Grasset, Shaasfsma e Hodgson, na África do Sul, que confirmam muitas idéias do autor e mostram a unidade universal do síndromo escorpiônico descrito no Brasil.

  20. The retinoblastoma protein binds to a family of E2F transcription factors

    DEFF Research Database (Denmark)

    Lees, J A; Saito, M; Vidal, M;

    1993-01-01

    for E2F-2 and E2F-3 were mapped to 1p36 and 6q22, respectfully, confirming their independence from E2F-1. However, the E2F-2 and E2F-3 proteins are closely related to E2F-1. Both E2F-2 and E2F-3 bound to wild-type but not mutant E2F recognition sites, and they bound specifically to the retinoblastoma...

  1. Managing the consequences of aggressive conservative treatment for refractory retinoblastoma with vitreous seeding

    OpenAIRE

    2014-01-01

    A 4 year-old girl with bilateral, non-familial retinoblastoma (RB) was referred to our care after primary enucleation OS and active tumor OD refractory to multiple therapies (intravenous chemotherapy, laser/cryotherapy, and I-125 plaque radiotherapy). Vitreous seeding OD, initially controlled by several sessions of Ophthalmic Artery Infusion Chemotherapy (OAIC) and periocular chemotherapy, recurred shortly thereafter. The patient underwent intravitreal (IVit) Melphalan injections achieving tu...

  2. Illumina next generation sequencing data and expression microarrays data from retinoblastoma and medulloblastoma tissues

    Science.gov (United States)

    García-Chequer, A.J.; Méndez-Tenorio, A.; Olguín-López, G.; Sánchez-Vallejo, C.; Isa, P.; Arias, C.F.; Torres, J.; Hernández-Angeles, A.; Ramírez-Ortiz, M.A.; Lara, C.; Cabrera-Muñoz, Ma.de.L.; Sadowinski-Pine, S.; Bravo-Ortiz, J.C.; Ramón-García, G.; Diegopérez-Ramírez, J.; Ramírez-Reyes, G.; Casarrubias-Islas, R.; Ramírez, J.; Orjuela, M.; Ponce-Castañeda, M.V.

    2016-01-01

    Retinoblastoma (Rb) is a pediatric intraocular malignancy and probably the most robust clinical model on which genetic predisposition to develop cancer has been demonstrated. Since deletions in chromosome 13 have been described in this tumor, we performed next generation sequencing to test whether recurrent losses could be detected in low coverage data. We used Illumina platform for 13 tumor tissue samples: two pools of 4 retinoblastoma cases each and one pool of 5 medulloblastoma cases (raw data can be found at http://www.ebi.ac.uk/ena/data/view/PRJEB6630). We first created an in silico reference profile generated from a human sequenced genome (GRCh37p5). From this data we calculated an integrity score to get an overview of gains and losses in all chromosomes; we next analyzed each chromosome in windows of 40 kb length, calculating for each window the log2 ratio between reads from tumor pool and in silico reference. Finally we generated panoramic maps with all the windows whether lost or gained along each chromosome associated to its cytogenetic bands to facilitate interpretation. Expression microarrays was done for the same samples and a list of over and under expressed genes is presented here. For this detection a significance analysis was done and a log2 fold change was chosen as significant (raw data can be found at http://www.ncbi.nlm.nih.gov/geo/accession number GSE11488). The complete research article can be found at Cancer Genetics journal (Garcia-Chequer et al., in press) [1]. In summary here we provide an overview with visual graphics of gains and losses chromosome by chromosome in retinoblastoma and medulloblastoma, also the integrity score analysis and a list of genes with relevant expression associated. This material can be useful to researchers that may want to explore gains and losses in other malignant tumors with this approach or compare their data with retinoblastoma. PMID:26937470

  3. Cloning and Sequence Analysis of Light Variable Region Gene of Anti-human Retinoblastoma Monoclonal Antibody

    Institute of Scientific and Technical Information of China (English)

    Xiufeng Zhong; Yongping Li; Shuqi Huang; Bo Ning; Chunyan Zhang; Jianliang Zheng; Guanguang Feng

    2002-01-01

    Purpose: To clone the variable region gene of light chain of monoclonal antibody against human retinoblastoma and to analyze the characterization of its nucleotide sequence as well as amino acid sequence.Methods: Total RNA was extracted from 3C6 hybridoma cells secreting specific monoclonal antibody(McAb)against human retinoblastoma(RB), then transcripted reversely into cDNA with olig-dT primers.The variable region of the light chain (VL) gene fragments was amplified using polymeerase chain reaction(PCR) and further cloned into pGEM(R) -T Easy vector. Then, 3C6 VL cDNA was sequenced by Sanger's method.Homologous analysis was done by NCBI BLAST.Results: The complete nucleotide sequence of 3C6 VL cDNA consisted of 321 bp encoding 107 amino acid residues, containing four workframe regions(FRs)and three complementarity-determining regions (CDRs) as well as the typical structure of two cys residues. The sequence is most homological to a member of the Vk9 gene family, and its chain utilizes the Jkl gene segment.Conclusion: The light chain variable region gene of the McAb against human RB was amplified successfully , which belongs to the Vk9 gene family and utilizes Vk-Jk1 gene rearrangement. This study lays a good basis for constructing a recombinant antibody and for making a new targeted therapeutic agents against retinoblastoma.

  4. Experimental research of RB94 gene transfection into retinoblastoma cells using ultrasound-targeted microbubble destruction.

    Science.gov (United States)

    Zheng, Min-Ming; Zhou, Xi-Yuan; Wang, Li-Ping; Wang, Zhi-Gang

    2012-06-01

    The purpose of this study was to explore the transfection of the recombinant expression plasmid pEGFP-C1/RB94 into human retinoblastoma cells (HXO-Rb44) using ultrasound-targeted microbubble destruction (UTMD). pEGFP-C1/RB94 was transfected into HXO-Rb44 in vitro by UTMD, with liposome as the positive control. After 24 to 72 h, the expression of the reporter gene enhanced green fluorescent protein (EGFP) was observed using fluorescent microscopy and flow cytometry. The cell viability of HXO-Rb44 was measured by a MTT assay. The mRNA and proteins of RB94, caspase-3 and Bax were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Moreover, the apoptosis rate and cell cycle progression of the cells were detected by flow cytometry. This study demonstrated that UTMD can enhance the transfection efficiency of RB94, which has an obvious impact on the inhibition of the growth process of retinoblastoma cells, suggesting that the combination of UTMD and RB94 compounds might be a useful tool for use in the gene therapy of retinoblastoma.

  5. The tumor suppressor gene retinoblastoma-1 is required for retinotectal development and visual function in zebrafish.

    Directory of Open Access Journals (Sweden)

    Michael Gyda

    Full Text Available Mutations in the retinoblastoma tumor suppressor gene (rb1 cause both sporadic and familial forms of childhood retinoblastoma. Despite its clinical relevance, the roles of rb1 during normal retinotectal development and function are not well understood. We have identified mutations in the zebrafish space cadet locus that lead to a premature truncation of the rb1 gene, identical to known mutations in sporadic and familial forms of retinoblastoma. In wild-type embryos, axons of early born retinal ganglion cells (RGC pioneer the retinotectal tract to guide later born RGC axons. In rb1 deficient embryos, these early born RGCs show a delay in cell cycle exit, causing a transient deficit of differentiated RGCs. As a result, later born mutant RGC axons initially fail to exit the retina, resulting in optic nerve hypoplasia. A significant fraction of mutant RGC axons eventually exit the retina, but then frequently project to the incorrect optic tectum. Although rb1 mutants eventually establish basic retinotectal connectivity, behavioral analysis reveals that mutants exhibit deficits in distinct, visually guided behaviors. Thus, our analysis of zebrafish rb1 mutants reveals a previously unknown yet critical role for rb1 during retinotectal tract development and visual function.

  6. Nutrição aplicada à atividade motora Nutrition applied to motor activity

    Directory of Open Access Journals (Sweden)

    Antonio Herbert Lancha Junior

    2011-12-01

    Full Text Available A Nutrição aplicada a atividade motora se divide em quatro áreas do movimento humano, a saber: esporte, educação física, dança, recreação/lazer. Essa definição conceitual diferencia a população alvo da intervenção nutricional. O organismo humano sempre apresentou o movimento como parte de sua atividade cotidiana e selecionou evolutivamente os organismos mais econômicos. Em contrapartida por conta de demandas, sociais, financeiras dentre outras a vida moderna impôs o sedentarismo como padrão de comportamento motor que aliado ao padrão genético de economia resultaram nas doenças modernas como obesidade, diabetes, etc. Assim a sociedade institucionalizou o movimento humano criando manifestações distintas descritas acima e suas necessidades específicas passaram a ser de interesse acadêmico/cientifico. Nutricionalmente os estudos se concentram no balanço energético, na necessidade de carboidratos, proteínas, lipídios assim como dos micronutrientes e outros compostos biologicamente ativos. Estes estudos definem estas substancias sob critérios de essencialidade ou efeito ergogênico superior a capacidade fisiológica. O primeiro determina mudanças nas necessidades nutricionais e o segundo substâncias consideradas ilícitas. No presente momento grande parte da comunidade cientifica dedicada à nutrição aplicada à atividade motora, dirige sua vocação na tentativa de descobrir as necessidades específicas provocadas pela pratica regular da atividade motora permitindo seu exercício regular para que a mesma propicie os benefícios na manutenção da saúde de forma plena nas quatro áreas descritas acima.Nutrition applied to motor activity is divided in four areas of human movement, namely: sports, physical education, dance, recreation/leisure. This conceptual definition differentiates the target population of nutritional intervention. The human body has always presented the movement as part of their daily activity and

  7. Supraselective intra-arterial chemotherapy: evaluation of treatment-related complications in advanced retinoblastoma

    Directory of Open Access Journals (Sweden)

    Lejla Mutapcic Vajzovic

    2011-02-01

    Full Text Available Lejla Mutapcic Vajzovic1, Timothy G Murray1, Mohammad A Aziz-Sultan2, Amy C Schefler1, Stacey Quintero Wolfe2, Ditte Hess1, Cristina E Fernandes3, Sander R Dubovy1,41Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA; 2Department of Neurological Surgery, University of Miami/Jackson Memorial Hospital, Miami, FL, USA; 3Department of Pediatrics, University of Miami Miller School of Medicine, Miami, FL, USA; 4Florida Lions Oculopathology Laboratory, Miami, FL, USA Purpose: The purpose of this study is to report the complication profile and safety evaluation of supraselective intra-arterial melphalan chemotherapy in children undergoing treatment with advanced retinoblastoma.Methods: Twelve eyes of 10 children with advanced retinoblastoma (Reese-Ellsworth Group Vb or International Classification Group D were treated with supraselective intra-ophthalmic artery infusion of melphalan. Eleven eyes of nine children had previously failed traditional management with systemic chemotherapy and laser ablation and underwent intra-ophthalmic artery infusion of melphalan as an alternative to enucleation. Serial ophthalmic examinations, retinal photography, and ultrasonographic imaging were used to evaluate treatment regime.Results: Ophthalmic artery cannulation was successfully performed in 12 eyes of 10 patients (total 16 times. Striking regression of tumor, subretinal and vitreous seeds were seen early in each case. No severe systemic side effects occurred. Grade III neutropenia was seen in one patient. No transfusions were required. Three patients developed a vitreous hemorrhage obscuring tumor visualization. One patient developed periocular edema associated with inferior rectus muscle inflammation per orbital MRI. This same patient had scattered intraretinal hemorrhages and peripapillary cotton wool spots consistent with a Purtscher’s-like retinopathy that resolved spontaneously. At the 6-month follow-up examination

  8. A visão lakatosiana da ciência, aplicada à tecnologia e à engenharia: o caso do telex nacional

    OpenAIRE

    Jorge Eugenio Renner

    2011-01-01

    Imre Lakatos tornou-se conhecido por sua metodologia para a condução das atividades científicas. Trata-se aqui de analisar como também o progresso tecnológico pode ser enfocado pela metodologia lakatosiana e se tal extensão de conceitos pode ser aplicada aos estudos do progresso da engenharia na produção de bens e serviços. Observa-se que Lakatos fundamentou seus critérios de progresso da ciência em características comuns a todas as atividades humanas, tendo-as aplicado em particular à matemá...

  9. Abnormalities in structure and expression of the retinoblastoma gene in small cell lung cancer cell lines and xenografts in nude mice

    DEFF Research Database (Denmark)

    Rygaard, K; Sorenson, G D; Pettengill, O S;

    1990-01-01

    The putative retinoblastoma gene (Rb) is a tumor suppressor gene which is believed to cause retinoblastomas when both alleles are inactivated, leading to lack of the encoded Mr 110,000-116,000 phosphoprotein. Inactivation of the Rb gene has also been found in several other tumor types, including...

  10. O termo estratégia: um conceito útil para a lingüística aplicada?

    Directory of Open Access Journals (Sweden)

    John Robert Schmitz

    2001-02-01

    Full Text Available

    O termo estratégia é usado em certos casos na literatura especializada de lingüística aplicada sem muita precisão, pois, para alguns pesquisadores, ele é uma técnica; para outros, é método, de ensino e, para outros ainda, refere-se aos hábitos de estudo ou estilo cognitivo dos aprendizes. Outro problema é a pletora de estratégias propostas sem rigor na demarcação de diferenças: estratégias de comunicação (communication strategies, estratégias de aprendizagem (learning strategies, estratégias de aquisição (acquisition strategies e estratégias de ensino (teaching strategies. Apesar desses problemas, as pesquisas na área de estratégias são promissoras, já que o treinamento explícito em sua utilização ensina os aprendizes a: (i saber usar novas estratégias, (ii avaliar a eficiência de diferentes tipos; e (iii decidir quando é procedente transferir uma determinada estratégia para uma nova situação.

  11. Linguística Aplicada: uma identidade construida nos CBLA Applied Linguistics: an identity constructed in the Brazilian Congresses of Applied Linguistics

    Directory of Open Access Journals (Sweden)

    Renata Archanjo

    2011-01-01

    Full Text Available Este artigo objetiva investigar a Linguística Aplicada (LA como um campo de estudos produtor de conhecimento que tem sofrido muitas transformações ao longo de sua trajetória de busca de uma identidade própria. Tais transformações são recuperadas nas vozes de suas pesquisas ao longo dos anos. Assim, esse estudo analisa o percurso histórico da LA, com base na produção científica apresentada nos Congressos Brasileiros de Linguística Aplicada (CBLA, buscando compreender os significados revelados pelas vozes sociais que constituem o discurso sobre seu fazer científico. A análise da transformação das áreas e subáreas temáticas de estudo nesse recorte temporal apontam para a diversidade e a riqueza dos caminhos desse campo de saber que lhe configuram uma identidade própria e única.This article aims at investigating the field of Applied Linguistics as an area which produces knowledge and which has been under many changes. These changes can be represented by the different voices of its research over the past years. Thus, this study analyzes the historical path of Applied Linguistics within the range of the Brazilian Congresses of Applied Linguistics, trying to understand the different meanings of the social voices of the scientific discourse on this field. The analyses of the evolution of these areas and subareas of study in such aforementioned congresses point towards the diversity and richness of the path of this researching field which constitute its own and unique identity.

  12. O conceito de lucro econômico no âmbito da contabilidade aplicada

    Directory of Open Access Journals (Sweden)

    Alessandra Hirano Fuji

    2004-12-01

    Full Text Available O estudo da Ciência Contábil e as definições de elementos financeiros, notadamente o lucro, são de grande relevância não somente para os estudiosos do meio acadêmico, mas também para aqueles que atuam no mercado financeiro e trabalham com as questões práticas da Contabilidade. O lucro contábil, que constitui basicamente o confronto entre receita realizada e custo consumido, é respaldado pelo conservadorismo, convenção da objetividade e Princípios Contábeis Geralmente Aceitos. O lucro econômico, que é o incremento do valor presente do patrimônio líquido, envolve aspectos subjetivos, mas é superior ao lucro contábil, mormente no processo decisório dos usuários internos e externos. A questão de pesquisa é se o conceito de lucro econômico encontra-se realmente difundido entre os profissionais da área contábil. O objetivo do trabalho é enfatizar a importância do conceito de lucro econômico e verificar qual o grau de assimilação, utilização e divulgação do conceito no âmbito da Contabilidade Aplicada. O trabalho, baseado em revisão da literatura e estudo exploratório mostra que o conceito de lucro econômico não é plenamente conhecido e utilizado pelos usuários da contabilidade.The study of accounting theory and the definitions of financial elements, especially profit, is highly relevant not only for academics, but also for those directly involved in practical activities related to applied accounting. Accounting profit is the confrontation between revenue and cost and is based on conservatism, objectivity and Generally Accepted Accounting Principles. Economic profit is the increase in the equity's present value and involves subjective aspects, but it is better than accounting profit, mainly in the context of internal and external users' decisionmaking process. The research question is whether the concept of economic profit is really widespread among accounting professionals. This study aims to emphasize the

  13. Novel retinoblastoma treatment avoids chemotherapy: the effect of optimally timed combination therapy with angiogenic and glycolytic inhibitors on LHBETATAG retinoblastoma tumors

    Directory of Open Access Journals (Sweden)

    Samuel K Houston

    2011-01-01

    Full Text Available Samuel K Houston1, Yolanda Piña1, Timothy G Murray1, Hinda Boutrid1, Colleen Cebulla2, Amy C Schefler1, Wei Shi1, Magda Celdran1, William Feuer1, Jaime Merchan3, Ted J Lampidis41Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA; 2Department of Ophthalmology, The Ohio State University, Columbus, OH, USA; 3Division of Hematology/Oncology, Department of Medicine, 4Department of Cell Biology and Anatomy, University of Miami Miller School of Medicine and Sylvester Comprehensive Cancer Center, Miami, FL, USAPurpose: The purpose of this study was to evaluate the effect of optimally timed combination treatment with angiogenic and glycolytic inhibitors on tumor burden, hypoxia, and angiogenesis in advanced retinoblastoma tumors.Methods: LHBETATAG mice (n = 30 were evaluated. Mice were divided into 5 groups (n = 6 and received injections at 16 weeks of age (advanced tumors with a saline, b anecortave acetate (AA, c 2-deoxyglucose (2-DG, d AA + 2-DG (1 day post-AA treatment, or e AA + 2-DG (1 week post-AA treatment. Eyes were enucleated at 21 weeks and tumor sections were analyzed for hypoxia, angiogenesis, and tumor burden.Results: Eyes treated with 2-DG 1 day post-AA injection showed a 23% (P = 0.03 reduction in tumor burden compared with 2-DG alone and a 61% (P < 0.001 reduction compared with saline-treated eyes. Eyes treated with 2-DG 1 week post-AA injection showed no significant decrease in tumor burden compared with 2-DG alone (P = 0.21 and a 56% (P < 0.001 decrease in comparison with saline-treated eyes. 2-DG significantly reduced the total density of new blood vessels in tumors by 44% compared to saline controls (P < 0.001, but did not affect the density of mature vasculature.Conclusions: Combination therapy with angiogenic and glycolytic inhibitors significantly enhanced tumor control. Synergistic effects were shown to be dependent on the temporal course of treatment

  14. Postguerra y contracultura. Identidades en la ética aplicada

    Directory of Open Access Journals (Sweden)

    López de la Vieja de la Torre, M. Teresa

    2006-12-01

    Full Text Available The article analyses the role of cultural identities in Applied Ethics. Biotechnologies have been actually valued from different perspectives in Europe and in the United States. Since Nazi research and the politics of racial hygiene have shown the role of science under dictatorship. Nazi Doctors acted as soldiers. Therefore The Nürnberg Code claimed respect for human rights in Medicine, to protect individuals in clinical research. However American Doctors considered the Code suitable for a different context. Norms for a “barbaric” research, far away from the American standards. But in the United States radiations experiments have ignored laws and moral standards. Decades later, the Constitutional Treaty for the European Union emphasizes the value of dignity and personal integrity in the fields of Medicine and Biology. The informed consent and the prohibition of Eugenics connect then the future and the past.El artículo analiza el papel de las identidades culturales en la Ética aplicada. Las biotecnologías han sido valoradas efectivamente desde distintas perspectivas, en Europa y en Estados Unidos. Pues la investigación y la política de higiene racial durante el Nacionalsocialismo mostraron el papel desempeñado por la ciencia en la dictadura. Los médicos nazis actuaron como soldados. Por ello, el Código de Nürnberg reclamó respecto por los derechos humanos en Medicina, a fin de proteger a los ciudadanos en la investigación. Los médicos norteamericanos consideraron que el Código era adecuado para otro contexto, sin embargo. Normas para una investigación “bárbara”, alejada de los estándares americanos. Pero en Estados Unidos los experimentos con radiación ignoraron leyes y estándares morales. Décadas mas tarde, el Tratado constitucional para la Unión Europea insiste en el valor de la dignidad y de la integridad personal en Medicina y Biología. El consentimiento informado y la prohibición de practicas eugen

  15. Transcriptional regulatory program in wild-type and retinoblastoma gene-deficient mouse embryonic fibroblasts during adipocyte differentiation

    DEFF Research Database (Denmark)

    Hakim-Weber, Robab; Krogsdam, Anne-M; Jørgensen, Claus;

    2011-01-01

    this dual role of pRb in the early and late stages of adipogenesis we used microarrays to perform a comprehensive systems-level analysis of the common transcriptional program of the classic 3T3-L1 preadipocyte cell line, wild-type mouse embryonic fibroblasts (MEFs), and retinoblastoma gene-deficient MEFs...... of experimental data and computational analyses pinpointed a feedback-loop between Pparg and Foxo1.To analyze the effects of the retinoblastoma protein at the transcriptional level we chose a perturbated system (Rb-/- MEFs) for comparison to the transcriptional program of wild-type MEFs. Gene ontology analysis......Although many molecular regulators of adipogenesis have been identified a comprehensive catalogue of components is still missing. Recent studies showed that the retinoblastoma protein (pRb) was expressed in the cell cycle and late cellular differentiation phase during adipogenesis. To investigate...

  16. Notificações aos acordos TBT e SPS: diferentes objetivos e resultados sobre o comércio internacional de agroalimentos

    OpenAIRE

    Fernanda Maria de Almeida; Marília Fernandes Maciel Gomes; Orlando Monteiro da Silva

    2014-01-01

    O objetivo deste trabalho é identificar os efeitos que os diferentes objetivos contidos nas notificações aplicadas aos acordos TBT e SPS da Organização Mundial do Comércio tiveram sobre o comércio internacional de diferentes produtos agroalimentares, entre 1996 e 2010. Para tanto, utilizou-se um modelo de gravidade que foi estimado pelo método de Seleção Amostral. Os resultados apontaram que as notificações com objetivos de aumento de informações aos consumidores contribuíram para o aumento d...

  17. REDES TECNOLÓGICAS APLICADAS EM EaD

    Directory of Open Access Journals (Sweden)

    Suélly Lima dos Santos

    2011-08-01

    Full Text Available Com o advento da internet e o aparecimento das novas tecnologias educacionais, conjunto de informações e comunicações (telefone, internet, rádio, vídeo, computador e televisão, ultrapassaram as fronteiras das quatro paredes da sala de aula, surgindo ambientes virtuais direcionadas à ampliação da participação dos serviços públicos relacionados ao ensino. À educação e à formação técnica e profissional permitiu-se o aumento da interatividade através da tecnologia, promovendo maneiras de acesso ao aluno utilizando computadores e/ou dispositivos móveis que viabiliza a Educação à Distância (EaD. Devido o grande crescimento do ensino à distância, as instituições já utilizam redes de dados para ampliar o acesso de aprendizagem. Com este espantoso aumento das redes, e da acelerada transmissão de informações aprender como elas funcionam é muito importante, além do mais usando a própria tecnologia para este fim. Como conclusão deste trabalho as mudanças relâmpadas da grande disponibilidade de dados no ambiente virtual é de enorme valia para o processo ensino-aprendizado.

  18. A retrospective review of visual outcome and complications in the treatment of retinoblastoma.

    LENUS (Irish Health Repository)

    O'Doherty, M

    2005-01-01

    The aim of this study was to look at the visual outcome and treatment complications of children diagnosed with Retinoblastoma during the years 1985-2003 inclusive. A retrospective review of all patients records was performed. Patient characteristics, treatment methods and complications were recorded. Twenty eight children presented to Temple street Hospital between 1985-2003. Six of these infants had bilateral tumours. The mean age at presentation was 23.7 months. Sixty-nine percent presented with Leucocoria, of these 33% also had a squint. The mean duration of symptoms was only known in 58% and this figure was approximately 19.8 months. Enucleation was performed in 24 eyes of 24 patients. Three patients required adjuvant chemotherapy post enucleation. Two eyes was treated with external beam radiation and one eye with plaque radiotherapy. One eye (second eye) was treated with systemic chemotherapy and radiation. Five eyes of three patients were treated with systemic chemotherapy followed by adjuvant Argon laser, cryotherapy and diode laser to each eye.The complications of each treatment group was recorded. The visual outcome in the salvaged eyes was favourable. There were no deaths recorded. Though chemotherapy with adjuvant local treatments provide adequate treatment for early tumours, enucleation still plays a major role in the treatment of Retinoblastoma. The total eye salvage rate in this study was 29% with an enucleation rate of 90% in unilateral cases and 33% in bilateral cases. Sixty-six percent of bilateral eyes affected were salvaged. Seventy-one percent of tumours were diagnosed after a parent noticed a gross abnormality of the eye. This highlights the possible need for screening for retinoblastoma in the infant population.

  19. Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes.

    Directory of Open Access Journals (Sweden)

    Xiaoyun Wu

    Full Text Available The retinoblastoma (Rb tumor suppressor acts with a number of chromatin cofactors in a wide range of species to suppress cell proliferation. The Caenorhabditis elegans retinoblastoma gene and many of these cofactors, called synMuv B genes, were identified in genetic screens for cell lineage defects caused by growth factor misexpression. Mutations in many synMuv B genes, including lin-35/Rb, also cause somatic misexpression of the germline RNA processing P granules and enhanced RNAi. We show here that multiple small RNA components, including a set of germline-specific Argonaute genes, are misexpressed in the soma of many synMuv B mutant animals, revealing one node for enhanced RNAi. Distinct classes of synMuv B mutants differ in the subcellular architecture of their misexpressed P granules, their profile of misexpressed small RNA and P granule genes, as well as their enhancement of RNAi and the related silencing of transgenes. These differences define three classes of synMuv B genes, representing three chromatin complexes: a LIN-35/Rb-containing DRM core complex, a SUMO-recruited Mec complex, and a synMuv B heterochromatin complex, suggesting that intersecting chromatin pathways regulate the repression of small RNA and P granule genes in the soma and the potency of RNAi. Consistent with this, the DRM complex and the synMuv B heterochromatin complex were genetically additive and displayed distinct antagonistic interactions with the MES-4 histone methyltransferase and the MRG-1 chromodomain protein, two germline chromatin regulators required for the synMuv phenotype and the somatic misexpression of P granule components. Thus intersecting synMuv B chromatin pathways conspire with synMuv B suppressor chromatin factors to regulate the expression of small RNA pathway genes, which enables heightened RNAi response. Regulation of small RNA pathway genes by human retinoblastoma may also underlie its role as a tumor suppressor gene.

  20. Detection of Tumor Suppressor Gene and Oncogene in SO-Rb_(50) Human Retinoblastoma Cell Line

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    Retinoblastoma (Rb) is the most common malignant'cancer of eye. So-Rb_(50) is the first Rb cell line established in China in 1988. It has passed to the 387th passage now. We collected cells of the 327th passage of SO-Rb_(50), purified its genomic DNA and detected it with Rb and c-myc cDNA probes respectively (normal human white blood cells DNA was the control). We found the Rb gene was deleted while c-myc gene was amplified three times. This provides a basis for further study of the regulation of tumor ...

  1. Ocular Salvage and Vision Preservation Using a Topotecan-Based Regimen for Advanced Intraocular Retinoblastoma.

    Science.gov (United States)

    Brennan, Rachel C; Qaddoumi, Ibrahim; Mao, Shenghua; Wu, Jianrong; Billups, Catherine A; Stewart, Clinton F; Hoehn, Mary Ellen; Rodriguez-Galindo, Carlos; Wilson, Matthew W

    2017-01-01

    Purpose To evaluate topotecan-based therapy for advanced intraocular retinoblastoma. Patients and Methods Twenty-seven patients with bilateral retinoblastoma (male patients, n = 14; median age, 8.4 months) received chemotherapy: two courses of topotecan plus vincristine followed by three alternating administrations of carboplatin and vincristine for two courses and topotecan plus vincristine for one course, with optional periocular carboplatin. Focal therapy was applied after cycle 2. Event-free survival was defined as avoidance of external-beam radiotherapy (EBRT) and enucleation. Results Of 54 eyes, 42 were Reese-Ellsworth group IV to V, and 37 were International Classification of Retinoblastoma group C to E. Eleven eyes were enucleated: one at diagnosis, nine with progressive disease including three eyes treated with EBRT, and one that developed neovascular glaucoma after completion of therapy. One patient was removed from protocol with prolonged infection in course 1; 26 patients (51 eyes) were analyzed. At 10 years, cumulative incidence of EBRT was 5.9% (SE ± 3), and event-free survival was 69.2% (SE ± 27.2). Ocular survival for Reese-Ellsworth group IV to V eyes was 77.5% (SE ± 21.2); it was 74.3% (SE ≥ 18.8) for International Classification group C to E eyes. Vision testing (median age, 7 years; range, 5 to 10 years) documented 20/70 vision or better in one eye of 23 patients; 19 (76%) of 25 patients demonstrated 20/40 vision or better in one eye. All patients experienced thrombocytopenia (41 episodes in 275 courses; 15%). There were 29 episodes of febrile neutropenia (10%). Grade 3 diarrhea was present in nine of 27 patients, and one patient had an allergic reaction to carboplatin. All patients are alive at median follow-up 7.4 years (range, 2.7 to 10 years). Conclusion Topotecan combined with vincristine, carboplatin, and aggressive focal therapies is an effective regimen for the treatment of advanced retinoblastoma and results in globe salvage with

  2. Efeito de cargas aplicadas e profundidades de semeadura no desenvolvimento da cultura do feijão em sistema plantio direto Effect of loads applied and depths of sowing in the development of bean culture in no-tillage system

    Directory of Open Access Journals (Sweden)

    Alcir José Modolo

    2010-06-01

    Full Text Available Dentre os fatores primordiais para a germinação estão a umidade, a temperatura e a aeração do solo. É importante citar que esses fatores são diretamente influenciados pelo estado de compactação do solo ao redor da semente. Objetivou-se, neste trabalho, estudar os efeitos da combinação entre profundidades de semeadura e cargas aplicadas pela roda compactadora da semeadora-adubadora sobre o desenvolvimento da cultura do feijão, em sistema plantio direto. Foi utilizado o esquema fatorial no delineamento em blocos ao acaso, composto por doze tratamentos (três profundidades de semeadura e quatro níveis de cargas aplicadas pela roda compactadora e quatro repetições. Os resultados mostraram que a profundidade de semeadura influenciou, significativamente, o índice de velocidade de emergência, os estandes inicial e final de plantas e o rendimento da cultura. As diferentes cargas aplicadas pela roda compactadora não influenciaram significativamente as variáveis avaliadas.Among the primordial factors for the germination are humidity, temperature, and aeration of the soil. It is important to mention that these factors are directly influenced by the soil compaction state around the seed. The present paper had the aim of studying the effects of the combination between depths of sowing and loads applied by press wheel of the seeder upon the development of bean culture, in a no-tillage system. Factor schedule was applied to the delineation in random blocks composed by twelve treatments (three depths of sowing and four levels of loads applied by the press wheel and four replications. The results showed that depth of sowing significantly influenced the index of emergency speed, the initial and final stands of plants and yield of culture. The different loads applied for the press wheel did not significantly influence the evaluated variables.

  3. Modelo An\\'alogo Ac\\'ustico ao Buraco Negro de Schwarzschild

    CERN Document Server

    Toniato, Júnior Diniz

    2011-01-01

    Um fluido em movimento pode agir sobre o som da mesma forma que os espa\\c{c}o-tempos curvos podem influenciar na trajet\\'oria da luz na relatividade geral. Com isso, pode-se descrever a propaga\\c{c}\\~ao dessas ondas sonoras atrav\\'es de uma m\\'etrica efetiva, sob a qual elas seguir\\~ao geod\\'esicas nulas. Esta disserta\\c{c}\\~ao faz uma revis\\~ao destes estudos concentrando-se em uma analogia ac\\'ustica para um buraco negro de Schwarzschild, demonstrando suas vantagens e limita\\c{c}\\~oes quando aplicada para o estudo da teoria de Hawking. A fluid in moviment can act on the sound the same way that curved space-time can influence on light trajectory in the general relativity. So, one can describe the propagation these sound waves through an effective metric, under wich they will follow null geodesics. This thesis makes a review of these studies focusing in an analogy to Schwarzschild black hole using an acoustic system, showing its advantages and limitations when applied in the study of Hawking's theory.

  4. Dimensão aplicada na análise do comportamento

    Directory of Open Access Journals (Sweden)

    Canaan-Oliveira Silvia

    2003-01-01

    Full Text Available O presente estudo teve como objetivo examinar a lógica do uso do termo "aplicada" na Análise do Comportamento a partir de elementos identificados na literatura desta área e na linguagem cotidiana. As concepções de aplicação encontradas foram categorizadas em três grupos: a aplicação enquanto prestação de serviço que leva a discussão para o continuum prestação de serviço X pesquisa, assemelhando-se à forma como o termo "aplicada" é utilizado na linguagem cotidiana, b aplicação enquanto uma forma de pesquisa que revela uma revisão do conceito de aplicação na linguagem cotidiana e sua estensão para atividades científicas, direcionando a discussão para o continuum pesquisa básica x pesquisa aplicada e c aplicação enquanto atividade simultânea de pesquisa e prestação de serviço que sinaliza o caráter complementar destas atividades. Portanto, existem tradições coexistentes na Análise do Comportamento que apoiam diferentes usos do termo "aplicada".

  5. Comparison of the diagnostic value of MR imaging and ophthalmoscopy for the staging of retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Khurana, Aman; Wan, Wenshuai; Yeom, Kristen [Stanford University, Department of Radiology, Stanford, CA (United States); Eisenhut, Christina A. [Technical University of Munich, Department of Radiology, Munich (Germany); Ebrahimi, Katayoon B. [Johns Hopkins University, Department of Ocular Oncology, Baltimore, MD (United States); Patel, Chirag [University of California Davis, Department of Radiology, Davis, CA (United States); O' Brien, Joan M. [University of Pennsylvania, Department of Ophthalmology, Philadelphia, PA (United States); Daldrup-Link, Heike E. [Stanford University, Department of Radiology, Stanford, CA (United States); Stanford University, Department of Radiology, Pediatric Radiology Section, Lucile Packard Children' s Hospital, Stanford, CA (United States)

    2013-05-15

    To compare the diagnostic value of magnetic resonance (MR) imaging and ophthalmoscopy for staging of retinoblastoma. MR and ophthalmoscopic images of 36 patients who underwent enucleation were evaluated retrospectively following institutional review board approval. Histopathology being the standard of reference, the sensitivity and specificity of both diagnostic modalities were compared regarding growth pattern, iris neoangiogenesis, retinal detachment, vitreous seeds and optic nerve invasion. Data were analysed via McNemar's test. Both investigations showed no significant difference in accuracy for the detection of different tumour growth patterns (P = 0.80). Vitreous seeding detection was superior by ophthalmoscopy (P < 0.001). For prelaminar optic nerve invasion, MR imaging showed similar sensitivity as ophthalmoscopy but increased specificity of 40 % (CI 0.12-0.74) vs. 20 % (0.03-0.56). MR detected optic nerve involvement past the lamina cribrosa with a sensitivity of 80 % (0.28-0.99) and a specificity of 74 % (0.55-0.88). The absence of optic nerve enhancement excluded histopathological infiltration, but the presence of optic nerve enhancement included a high number of false positives (22-24 %). Ophthalmoscopy remains the method of choice for determining extent within the globe while MR imaging is useful for evaluating extraocular tumour extension. Thus, both have their own strengths and contribute uniquely to the staging of retinoblastoma. (orig.)

  6. Managing the consequences of aggressive conservative treatment for refractory retinoblastoma with vitreous seeding

    Directory of Open Access Journals (Sweden)

    Aubrey Brink

    2014-08-01

    Full Text Available A 4 year-old girl with bilateral, non-familial retinoblastoma (RB was referred to our care after primary enucleation OS and active tumor OD refractory to multiple therapies (intravenous chemotherapy, laser/cryotherapy, and I-125 plaque radiotherapy. Vitreous seeding OD, initially controlled by several sessions of Ophthalmic Artery Infusion Chemotherapy (OAIC and periocular chemotherapy, recurred shortly thereafter. The patient underwent intravitreal (IVit Melphalan injections achieving tumor control despite the concurrent development of keratopathy, pupillary synechiae, cataract, and necrosis of the inferior fornix and the adjacent orbital fat, all secondary to the treatments administered. Repeated amniotic membrane implants and tarsorrhaphy were performed to alleviate the symptoms. Despite being tumor free for 6 months, a poor fundus view and treatment-related complications prompted us to consider enucleation, but parents declined. Following recent negative magnetic resonance imaging (MRI, her cataract was removed. She was then found to have tumor recurrence. Her eye was enucleated 12 months ago and she recovered well from the surgery. As ocular oncology embarks in eye-preserving treatments for retinoblastoma, it is important to address the cumulative effects and associated impact of such treatments and the possibility of failure.

  7. Salvage/Adjuvant Brachytherapy After Ophthalmic Artery Chemosurgery for Intraocular Retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Francis, Jasmine H., E-mail: francij1@mskcc.org [Memorial Sloan-Kettering Cancer Center, New York, New York (United States); Barker, Christopher A.; Wolden, Suzanne L.; McCormick, Beryl; Segal, Kira; Cohen, Gil [Memorial Sloan-Kettering Cancer Center, New York, New York (United States); Gobin, Y. Pierre; Marr, Brian P. [Memorial Sloan-Kettering Cancer Center, New York, New York (United States); Weill-Cornell Medical College, New York-Presbyterian Hospital, New York, New York (United States); Brodie, Scott E. [Memorial Sloan-Kettering Cancer Center, New York, New York (United States); Mount Sinai School of Medicine, New York, New York (United States); Dunkel, Ira J.; Abramson, David H. [Memorial Sloan-Kettering Cancer Center, New York, New York (United States); Weill-Cornell Medical College, New York-Presbyterian Hospital, New York, New York (United States)

    2013-11-01

    Purpose: To evaluate the efficacy and toxicity of brachytherapy after ophthalmic artery chemosurgery (OAC) for retinoblastoma. Methods and Materials: This was a single-arm, retrospective study of 15 eyes in 15 patients treated with OAC followed by brachytherapy at (blinded institution) between May 1, 2006, and December 31, 2012, with a median 19 months' follow-up from plaque insertion. Outcome measurements included patient and ocular survival, visual function, and retinal toxicity measured by electroretinogram (ERG). Results: Brachytherapy was used as adjuvant treatment in 2 eyes and as salvage therapy in 13 eyes of which 12 had localized vitreous seeding. No patients developed metastasis or died of retinoblastoma. The Kaplan-Meier estimate of ocular survival was 79.4% (95% confidence interval 48.7%-92.8%) at 18 months. Three eyes were enucleated, and an additional 6 eyes developed out-of-target volume recurrences, which were controlled with additional treatments. Patients with an ocular complication had a mean interval between last OAC and plaque of 2.5 months (SD 2.3 months), which was statistically less (P=.045) than patients without ocular complication who had a mean interval between last OAC and plaque of 6.5 months (SD 4.4 months). ERG responses from pre- versus postplaque were unchanged or improved in more than half the eyes. Conclusions: Brachytherapy following OAC is effective, even in the presence of vitreous seeding; the majority of eyes maintained stable or improved retinal function following treatment, as assessed by ERG.

  8. Illumina next generation sequencing data and expression microarrays data from retinoblastoma and medulloblastoma tissues

    Directory of Open Access Journals (Sweden)

    A.J. García-Chequer

    2016-03-01

    We first created an in silico reference profile generated from a human sequenced genome (GRCh37p5. From this data we calculated an integrity score to get an overview of gains and losses in all chromosomes; we next analyzed each chromosome in windows of 40 kb length, calculating for each window the log2 ratio between reads from tumor pool and in silico reference. Finally we generated panoramic maps with all the windows whether lost or gained along each chromosome associated to its cytogenetic bands to facilitate interpretation. Expression microarrays was done for the same samples and a list of over and under expressed genes is presented here. For this detection a significance analysis was done and a log2 fold change was chosen as significant (raw data can be found at http://www.ncbi.nlm.nih.gov/geo/accession number GSE11488. The complete research article can be found at Cancer Genetics journal (Garcia-Chequer et al., in press [1]. In summary here we provide an overview with visual graphics of gains and losses chromosome by chromosome in retinoblastoma and medulloblastoma, also the integrity score analysis and a list of genes with relevant expression associated. This material can be useful to researchers that may want to explore gains and losses in other malignant tumors with this approach or compare their data with retinoblastoma.

  9. Novel roles of plant RETINOBLASTOMA-RELATED (RBR) protein in cell proliferation and asymmetric cell division.

    Science.gov (United States)

    Desvoyes, Bénédicte; de Mendoza, Alex; Ruiz-Trillo, Iñaki; Gutierrez, Crisanto

    2014-06-01

    The retinoblastoma (Rb) protein was identified as a human tumour suppressor protein that controls various stages of cell proliferation through the interaction with members of the E2F family of transcription factors. It was originally thought to be specific to animals but plants contain homologues of Rb, called RETINOBLASTOMA-RELATED (RBR). In fact, the Rb-E2F module seems to be a very early acquisition of eukaryotes. The activity of RBR depends on phosphorylation of certain amino acid residues, which in most cases are well conserved between plant and animal proteins. In addition to its role in cell-cycle progression, RBR has been shown to participate in various cellular processes such as endoreplication, transcriptional regulation, chromatin remodelling, cell growth, stem cell biology, and differentiation. Here, we discuss the most recent advances to define the role of RBR in cell proliferation and asymmetric cell division. These and other reports clearly support the idea that RBR is used as a landing platform of a plethora of cellular proteins and complexes to control various aspects of cell physiology and plant development.

  10. Sonic hedgehog initiates cochlear hair cell regeneration through downregulation of retinoblastoma protein

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Na [Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai 200031 (China); Department of Otolaryngology and Program in Neuroscience, Harvard Medical School and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA 02114 (United States); Chen, Yan [Central Laboratory, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai 200031 (China); Wang, Zhengmin [Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai 200031 (China); Institute of Biomedical Sciences, Fudan University, Shanghai 200032 (China); Chen, Guoling [Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai 200031 (China); Lin, Qin [Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai 200031 (China); Department of Otolaryngology, First Affiliated Hospital of Fujian Medical University, Otolaryngology Institute of Fujian Province, Fuzhou (China); Chen, Zheng-Yi, E-mail: Zheng-yi_chen@meei.harvard.edu [Department of Otolaryngology and Program in Neuroscience, Harvard Medical School and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA 02114 (United States); Li, Huawei, E-mail: hwli@shmu.edu.cn [Otology Skull Base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai 200031 (China); Institute of Biomedical Sciences, Fudan University, Shanghai 200032 (China)

    2013-01-11

    Highlights: Black-Right-Pointing-Pointer Shh activation in neonatal cochleae enhances sensory cell proliferation. Black-Right-Pointing-Pointer Proliferating supporting cells can transdifferentiate into hair cells. Black-Right-Pointing-Pointer Shh promotes proliferation by transiently modulating pRb activity. Black-Right-Pointing-Pointer Shh inhibits pRb by inhibiting transcription and increasing phosphorylation of pRb. -- Abstract: Cell cycle re-entry by cochlear supporting cells and/or hair cells is considered one of the best approaches for restoring hearing loss as a result of hair cell damage. To identify mechanisms that can be modulated to initiate cell cycle re-entry and hair cell regeneration, we studied the effect of activating the sonic hedgehog (Shh) pathway. We show that Shh signaling in postnatal rat cochleae damaged by neomycin leads to renewed proliferation of supporting cells and hair cells. Further, proliferating supporting cells are likely to transdifferentiate into hair cells. Shh treatment leads to inhibition of retinoblastoma protein (pRb) by increasing phosphorylated pRb and reducing retinoblastoma gene transcription. This results in upregulation of cyclins B1, D2, and D3, and CDK1. These results suggest that Shh signaling induces cell cycle re-entry in cochlear sensory epithelium and the production of new hair cells, in part by attenuating pRb function. This study provides an additional route to modulate pRb function with important implications in mammalian hair cell regeneration.

  11. Splicing aberrations caused by constitutional RB1 gene mutations in retinoblastoma

    Indian Academy of Sciences (India)

    Vidya Latha Parsam; Mohammed Javed Ali; Santosh G Honavar; Geeta K Vemuganti; Chitra Kannabiran

    2011-06-01

    Analysis of RB1 mRNA from blood leukocytes of patients with retinoblastoma identified the effects of mutations involving consensus splice site, exonic substitution and whole-exon deletions identified in genomic DNA of these patients. In addition, this study identified mutations in cases in which no mutations were detectable in the genomic DNA. One proband had mutation at the canonical splice site at +5 position of IVS22, and analysis of the transcripts in this family revealed skipping of exon 22 in three members of this family. In one proband, a missense substitution of c.652T > G (g.56897T > G; Leu218Val) in exon 7 led to splicing aberrations involving deletions of exons 7 and 8, suggesting the formation of a cryptic splice site. In two probands with no detectable changes in the genomic DNA upon screening of RB1 exons and flanking intronic sequences, transcripts were found to have deletions of exon 6 in one, and exons 21 and 22 in another family. In two probands, RNA analysis confirmed genomic deletions involving one or more exons. This study reveals novel effects of RB1 mutations on splicing and suggests the utility of RNA analysis as an adjunct to mutational screening of genomic DNA in retinoblastoma.

  12. Transcriptional regulation of Sox2 by the retinoblastoma family of pocket proteins.

    Science.gov (United States)

    Vilas, Jéssica M; Ferreirós, Alba; Carneiro, Carmen; Morey, Lluis; Da Silva-Álvarez, Sabela; Fernandes, Tânia; Abad, María; Di Croce, Luciano; García-Caballero, Tomás; Serrano, Manuel; Rivas, Carmen; Vidal, Anxo; Collado, Manuel

    2015-02-20

    Cellular reprogramming to iPSCs has uncovered unsuspected links between tumor suppressors and pluripotency factors. Using this system, it was possible to identify tumor suppressor p27 as a repressor of Sox2 during differentiation. This led to the demonstration that defects in the repression of Sox2 can contribute to tumor development. The members of the retinoblastoma family of pocket proteins, pRb, p107 and p130, are negative regulators of the cell cycle with tumor suppressor activity and with roles in differentiation. In this work we studied the relative contribution of the retinoblastoma family members to the regulation of Sox2 expression. We found that deletion of Rb or p130 leads to impaired repression of Sox2, a deffect amplified by inactivation of p53. We also identified binding of pRb and p130 to an enhancer with crucial regulatory activity on Sox2 expression. Using cellular reprogramming we tested the impact of the defective repression of Sox2 and confirmed that Rb deficiency allows the generation of iPSCs in the absence of exogenous Sox2. Finally, partial depletion of Sox2 positive cells reduced the pituitary tumor development initiated by Rb loss in vivo. In summary, our results show that Sox2 repression by pRb is a relevant mechanism of tumor suppression.

  13. Ethnic variations of a retinoblastoma susceptibility gene (RB1) polymorphism in eight Asian populations

    Indian Academy of Sciences (India)

    Priya Kadam-Pai; Xin-Yi Su; Jasmin Jiji Miranda; Agustinus Soemantri; Nilmani Saha; Chew-Kiat Heng; Poh-San Lai

    2003-04-01

    An A → G single nucleotide polymorphism (SNP) at nucleotide 153,104 in the retinoblastoma susceptibility locus (RB1) at 13q14 was previously reported to be present only in Asians. In this study, we determined the distribution of this SNP in normal Southeast Asian populations (Chinese, Malay, Javanese, Thai, Filipino), in South Asian populations (Bangladeshi, Pakistani Pushtun and Indian) and in Chinese retinoblastoma cases and control subjects. The RB1 SNP was present in all populations at an overall frequency of ≤ 0.18. Heterozygosity was higher in the Southeast Asian groups (0.14–0.34) than in the South Asian groups (Bangladeshi and Indian) (0.04–0.06). Significant differences in allele frequencies were found between the two population groups. Interestingly, our Pakistani population comprised of ethnic Pushtuns from northwest Pakistan was significantly different from the neighbouring Bangladeshi and Indian populations. No significant difference was found between Chinese case patients and control subjects. This RB1 SNP appears to be an ethnic variant prevalent in Southeast Asian populations and may be useful for studying RB1 inheritance by pedigree analysis.

  14. Transcriptional regulation of Sox2 by the retinoblastoma family of pocket proteins

    Science.gov (United States)

    Carneiro, Carmen; Morey, Lluis; Silva-Álvarez, Sabela Da; Fernandes, Tânia; Abad, María; Croce, Luciano Di; García-Caballero, Tomás; Serrano, Manuel; Rivas, Carmen; Vidal, Anxo; Collado, Manuel

    2015-01-01

    Cellular reprogramming to iPSCs has uncovered unsuspected links between tumor suppressors and pluripotency factors. Using this system, it was possible to identify tumor suppressor p27 as a repressor of Sox2 during differentiation. This led to the demonstration that defects in the repression of Sox2 can contribute to tumor development. The members of the retinoblastoma family of pocket proteins, pRb, p107 and p130, are negative regulators of the cell cycle with tumor suppressor activity and with roles in differentiation. In this work we studied the relative contribution of the retinoblastoma family members to the regulation of Sox2 expression. We found that deletion of Rb or p130 leads to impaired repression of Sox2, a deffect amplified by inactivation of p53. We also identified binding of pRb and p130 to an enhancer with crucial regulatory activity on Sox2 expression. Using cellular reprogramming we tested the impact of the defective repression of Sox2 and confirmed that Rb deficiency allows the generation of iPSCs in the absence of exogenous Sox2. Finally, partial depletion of Sox2 positive cells reduced the pituitary tumor development initiated by Rb loss in vivo. In summary, our results show that Sox2 repression by pRb is a relevant mechanism of tumor suppression. PMID:25576924

  15. Do Desenho ao Design: um percurso semiótico?

    Directory of Open Access Journals (Sweden)

    Lucrécia d'Aléssio Ferrara

    2008-11-01

    Full Text Available Estudo do confronto entre as caracterí­sticas do desenho industrial como linguagem e sua possí­vel semiose no percurso que o leva a atingir o design como dimensão cognitiva que qualifica o desenho e o designer transformando-os em manifestações que interferem na dinâmica cultural do mundo urbano industrial e pós-industrial. Nesse confronto, estuda-se a ambigüidade que se pode encontrar entre estudos que utilizam a semiótica como lógica da linguagem inerente aos processos de semiose ou aqueles que a consideram um método aplicativo linear e útil í prática do projeto. Procura-se, portanto, estudar duas questões: trata-se de uma semiótica aplicada ao design ou de uma semiótica do design? Palavras-chave design, conhecimento, informação, semiótica, comunicação, desenho industrial Abstract This paper attempts at showing the confront between the characteristics of industrial design as a language and its possible semiosis that leads it to reach the design as a cognitive process. This semiosis qualifies both the drawing and the designer as cultural interferences in an industrial and postindustrial world. In this confront we examine the ambiguity that may be found between studies that makes use of semiotics as logic of the language inherent to the processes of semiosis, and those which consider it a linear applicative method, useful to the practice of the project. Thus, we aim considering two issues: is it a semiotics applied to the design or else a semiotics of the design? Key words design, knowledge, information, semiotic,communication, industrial design

  16. 视网膜母细胞瘤的治疗进展%Recent advances in retinoblastoma treatment

    Institute of Scientific and Technical Information of China (English)

    刘瑾; 朱豫

    2013-01-01

    随着人们对视觉功能、面部外观及生活质量的重视,手术摘除患眼治疗视网膜母细胞瘤的传统治疗方法已受到挑战.视网膜母细胞瘤的早期诊断、早期治疗、保留眼球和有用视力,现已成为新的追求.光凝、超低温冷冻、经瞳孔温热治疗等局部治疗方法逐渐受到重视,化疗联合局部治疗成为趋势.现将其治疗方法及研究进展综述如下.%As a traditional method of retinoblastoma treatment, simple eyeball enu-cleation has been challenged since people pay more attention to visual function, facial appearance and quality of life. Early diagnosis and treatment, preserving eyeball and useful vision have been as the new goal for retinoblastoma treatment. Currently, focal therapies including photocoagulation, cryotherapy, transpupillary thermotherapy play an important role in retinoblastoma treatment. This article reviews the recent advances in retinoblastoma treatment.

  17. Activation of peroxisome proliferator-activated receptor gamma bypasses the function of the retinoblastoma protein in adipocyte differentiation

    DEFF Research Database (Denmark)

    Hansen, Jacob B.; Petersen, R K; Larsen, B M;

    1999-01-01

    The retinoblastoma protein (pRB) is an important regulator of development, proliferation, and cellular differentiation. pRB was recently shown to play a pivotal role in adipocyte differentiation, to interact physically with adipogenic CCAAT/enhancer-binding proteins (C/EBPs), and to positively...

  18. MEMS AO for Planet Finding

    Science.gov (United States)

    Rao, Shanti; Wallace, J. Kent; Shao, Mike; Schmidtlin, Edouard; Levine, B. Martin; Samuele, Rocco; Lane, Benjamin; Chakrabarti, Supriya; Cook, Timothy; Hicks, Brian; Jung, Paul

    2008-01-01

    This slide presentation reviews a method for planet finding using microelectromechanical systems (MEMS) Adaptive Optics (AO). The use of a deformable mirror (DM) is described as a part of the instrument that was designed with a nulling interferometer. The strategy that is used is described in detail.

  19. A inserção do ensino de custos na disciplina administração aplicada à enfermagem La inserción de la enseñanza de costos en la disciplina administración aplicada a la enfermería Insertion of cost teaching in the discipline of administration applied to nursing

    Directory of Open Access Journals (Sweden)

    Ivone Maria Fonseca Francisco

    2006-03-01

    Full Text Available O objetivo deste texto foi realizar um resgate teórico sobre a traje-tória da disciplina de Admi-nistração Aplicada à Enfermagem, na graduação, ao longo da história do ensino da Enfermagem Brasi-leira, relacionando-a ao ensino de Custos. Percebemos, inseridas no ensino dessa disciplina, diversas citações diretas referentes ao tema Custos ou Economia em Saúde, desde o primeiro currículo de Enfermagem de 1890 até as Diretrizes Curriculares Nacionais do Curso de Graduação em Enfer-magem de 2001, além de citações indiretas relacionadas ao geren-ciamento, tomada de decisão, assessorias em projetos de saúde, entre outras, mas sem haver uma continuidade.Este texto tuvo como objetivo realizar un rescate teórico sobre la trayectoria de la disciplina de Administración Aplicada a la enfermería, en el pregrado, a lo largo de la historia de la enseñanza de la Enfermería Brasileña, relacionándola a la enseñanza de Costos. Percibimos, insertadas en la enseñanza de esa disciplina, diversas citaciones directas referentes al tema Costos o Economía en Salud, desde el primer currículo de Enfermería de 1890 hasta las Directivas Curriculares Nacionales del Pregrado en Enfermería del 2001, además de citaciones indirectas relacionadas al gerenciamiento, toma de decisión, asesorías en proyectos de salud, entre otras, pero sin ninguna continuidad.The objective of this text was to make theoretical rescue of the course of the undergraduate disci-pline Administration Applied to Nursing throughout the history of teaching of Nursing in Brazil, connecting it to the teaching of Costs. We noticed, inserted in the teaching of this discipline, many direct quotes related to the themes of Costs or Economics in Health, from the earliest curriculum in Nursing, dating back to 1890, to the National Curricular Policies of the Nursing Undergraduate Course, of 2001, in addition to indirect quotes related to mana-gement, decision taking, and advising in

  20. La teoría de los conjuntos-T aplicada al desarrollo de la competencia de modelado matemático

    Directory of Open Access Journals (Sweden)

    RICARDO JOAQUÍN DE ARMAS COSTA

    2016-06-01

    Full Text Available En este artículo queremos dar a conocer apartes de la teoría de los conjuntos-T y cómo se aplica en la educación superior para desarrollar la competencia de modelado matemático de los sujetos. Esta teoría surge como respuesta a los problemas de enseñanza aprendizaje de las matemáticas que se originan en los programas curriculares diseñados para adquirir exclusivamente información y no para desarrollar las competencias matemáticas de los estudiantes. The theory of the Conjuntos-T applied to the development of the competition of mathematical modeling In this article we want to present parts of the theory of joint-T and how it is applied in higher education to develop the competence of mathematical modeling subjects. We explain how the theory can develop the skills of students. This theory is a response to the problems of learning of mathematics that originate in the curriculum designed to acquire information and not only to develop the math skills of students. A teoria de Conjuntos-T aplicada ao desenvolvimento da concorrência  modelagem matemática Neste artigo, nos queremos apresentar algumas noções da teoria dos conjuntos-T e como ela é aplicada na docência, de ensino superior, para desenvolver a competência da modelagem matemática. Nos vamos explicar como a teoria pode desenvolver as habilidades dos alunos. Esta teoria é uma resposta para os problemas de ensino e aprendizagem da matemática que se originam nos programas curriculares destinados a adquirir somente informação e não para desenvolver as habilidades matemáticas dos alunos.

  1. Biotechnology applied to salmoniculture Biotecnologia aplicada à salmonicultura

    Directory of Open Access Journals (Sweden)

    Iván Valdebenito Isler

    2009-07-01

    machos. Essas ações são realizadas com GnRHa em doses próximas de 10ìg/kg de peixe. Após a maturidade sexual, a manipulação de gametas "in vitro" deve ser feita, quer seja devido ao armazenamento prolongado (particularmente "milt", pois têm que ser transportado ao local em que ocorrerá a fecundação, ou para aguardar os resultados ictiopatológicos, geralmente tomada dos reprodutores. A produção de população "todas fêmeas" também é comum. Frequentemente, estas populações em truta arcoíris são triplóides (por meio de choque temperatura próxima a 28 ºC ou pressão próxima de 8.000 psi para obter espécies estéreis que melhoram desempenho produtivo das populações cultivadas sem sinais de maturidade sexual. Além disso, as perspectivas de utilização industrial de organismos transgênicos na criação de salmonídeos são analisadas.

  2. TERMOGRAFIA MÉDICA INFRAVERMELHA APLICADA À MEDICINA DO ESPORTE

    Directory of Open Access Journals (Sweden)

    Ana Carolina Ramos e Côrte

    Full Text Available RESUMO A termografia médica infravermelha é um instrumento de análise não invasiva e não radioativa, capaz de analisar funções fisiológicas relacionadas com o controle da temperatura da pele. A termografia detecta a luz infravermelha emitida pelo corpo e visualiza mudanças de temperatura corporal relacionadas à alteração no fluxo sanguíneo. Não é um método que mostra anormalidades anatômicas, porém é capaz de mostrar mudanças fisiológicas. Existem várias aplicações da termografia no campo da medicina: desordens neurológicas, reumatológicas, musculares, doenças vasculares, patologias urológicas, ginecológicas, ortopédicas e na medicina esportiva. Para todas as áreas médicas, a termografia está estabelecida como uma medida que proporciona um mapeamento visual da distribuição da temperatura da pele. A termografia não deve ser usada como ferramenta diagnóstica única. Exames clínicos devem ser realizados para interpretação dos termogramas. Nas aplicações médicas, esta técnica proporciona, somente, uma imagem da distribuição da temperatura da pele; não é capaz de mostrar dados de uma superfície profunda do corpo, como é possível por outros exames de imagem. Entretanto, é um método não-invasivo e objetivo, além de seguro e inofensivo. Na medicina do esporte, o uso da termografia pode proporcionar melhores resultados aos atletas por ser um instrumento na identificação de riscos e na prevenção de lesões, além de ser uma importante ferramenta no acompanhamento do treinamento esportivo, a partir da avaliação da quantificação da carga de trabalho.

  3. Cometas: Das Lendas aos Fatos

    Science.gov (United States)

    Voelzke, M. R.

    O descobrimento de cometas, devido ao seu aparecimento espetacular, tem registro nas mais antigas culturas humanas. A primeira referência situa-se no ano de 1095 antes de Cristo [a.C.; HO; HO, 1962]. A quantidade de registros de descobrimentos cometários, principalmente provenientes do território chinês em particular e do oriente em geral, aumentou gradualmente a partir do quarto século depois de Cristo (d.C.). É de origem chinesa a primeira referência ao cometa P/Halley no ano de 240 a.C. [VOELZKE, 1993]. Com o desenvolvimento da astronomia relativamente às técnicas observacionais os descobrimentos bem como as observações cometárias aumentaram sensivelmente a partir do século XVII, sendo que a partir do século XIX um novo incremento ocorreu devido ao emprego da fotografia e a resultante melhora de sensibilidade na observação.

  4. Spontaneously resolving periocular erythema and ciliary madarosis following intra-arterial chemotherapy for retinoblastoma

    Directory of Open Access Journals (Sweden)

    Marr Brian

    2010-01-01

    Full Text Available Purpose and Design: To describe an unusual clinical finding seen in children undergoing intra-arterial chemotherapy for retinoblastoma. Materials and Methods: A retrospective review of 69 eyes of 63 patients receiving intra-arterial chemotherapy over a 3-year period. Charts and photographs of 69 consecutive cases were reviewed, and data were collected on patients with clinical evidence of a hyperemic cutaneous periocular abnormality following the procedure. Results: A blanching erythematous and edematous patch was noted in the periocular region in 16% (11 of 69 of the children who received intraarterial chemotherapy. The plaque extended into the region of the supertrochlear and medial marginal artery distribution on the ipsilateral side of the intra-arterial chemotherapy. All patches of erythema spontaneously resolved within 3 months following completion of the intra-arterial chemotherapy. Conclusion: Periocular erythema and swelling is a self-limited clinical finding associated with intra-arterial chemotherapy in a small number of patients.

  5. Retinoblastoma protein co-purifies with proteasomal insulin-degrading enzyme: Implications for cell proliferation control

    Energy Technology Data Exchange (ETDEWEB)

    Radulescu, Razvan T., E-mail: ratura@gmx.net [Molecular Concepts Research (MCR), Muenster (Germany); Duckworth, William C. [Department of Medicine, Phoenix VA Health Care System, Phoenix, AZ (United States); Levy, Jennifer L. [Research Service, Phoenix VA Health Care System, Phoenix, AZ (United States); Fawcett, Janet, E-mail: janet.fawcett@va.gov [Research Service, Phoenix VA Health Care System, Phoenix, AZ (United States)

    2010-04-30

    Previous investigations on proteasomal preparations containing insulin-degrading enzyme (IDE; EC 3.4.24.56) have invariably yielded a co-purifying protein with a molecular weight of about 110 kDa. We have now found both in MCF-7 breast cancer and HepG2 hepatoma cells that this associated molecule is the retinoblastoma tumor suppressor protein (RB). Interestingly, the amount of RB in this protein complex seemed to be lower in HepG2 vs. MCF-7 cells, indicating a higher (cytoplasmic) protein turnover in the former vs. the latter cells. Moreover, immunofluorescence showed increased nuclear localization of RB in HepG2 vs. MCF-7 cells. Beyond these subtle differences between these distinct tumor cell types, our present study more generally suggests an interplay between RB and IDE within the proteasome that may have important growth-regulatory consequences.

  6. Retinoblastoma pathway defects show differential ability to activate the constitutive DNA damage response in human tumorigenesis

    DEFF Research Database (Denmark)

    Tort, F.; Bartkova, J.; Sehested, M.

    2006-01-01

    Loss of G(1)-S control and aberrations of the p16(Ink4a)-cyclin D1/cyclin-dependent kinase (CDK) 4(6)-pRb-E2F-cyclin E/CDK2 pathway are common in human cancer. Previous studies showed that oncogene-induced aberrant proliferation, such as on cyclin E overexpression, causes DNA damage and checkpoint...... culture models with differential defects of retinoblastoma pathway components, as overexpression of cyclin D1 or lack of p16(Ink4a), either alone or combined, did not elicit detectable DDR. In contrast, inactivation of pRb, the key component of the pathway, activated the DDR in cultured human or mouse...... cells, analogous to elevated cyclin E. These results highlight differential effect of diverse oncogenic events on driving the 'cancer cell cycles' and their ability to deregulate the replication-driving CDK2 kinase and to alarm the DDR as a potential anticancer barrier in accordance...

  7. Deregulated MAPK activity prevents adipocyte differentiation of fibroblasts lacking the retinoblastoma protein

    DEFF Research Database (Denmark)

    Hansen, Jacob B; Petersen, Rasmus K; Jørgensen, Claus;

    2002-01-01

    A functional retinoblastoma protein (pRB) is required for adipose conversion of preadipocyte cell lines and primary mouse embryo fibroblasts (MEFs) in response to treatment with standard adipogenic inducers. Interestingly, lack of functional pRB in MEFs was recently linked to elevated Ras activity....../Akt are significantly increased in pRB-deficient MEFs both before and after the addition of adipogenic inducers. Consistently, we detected higher levels of activated Ras in MEFs lacking pRB. Suppression of ERK1/2 activation by the MEK inhibitor UO126 restored the ability of pRB-deficient MEFs to undergo adipocyte...... differentiation, as manifested by expression of adipocyte marker genes and lipid accumulation. Furthermore and reflecting the elevated levels of activated PKB/Akt in the pRB-deficient MEFs, differentiation proceeded in an insulin-independent manner. In conclusion, we suggest that pRB plays a pivotal role...

  8. Attenuation of disease phenotype through alternative translation initiation in low-penetrance retinoblastoma.

    Science.gov (United States)

    Sánchez-Sánchez, Francisco; Ramírez-Castillejo, Carmen; Weekes, Daniel B; Beneyto, Magdalena; Prieto, Félix; Nájera, Carmen; Mittnacht, Sibylle

    2007-02-01

    Hereditary predisposition to retinoblastoma (RB) is caused by germline mutations in the retinoblastoma 1 (RB1) gene and transmits as an autosomal dominant trait. In the majority of cases disease develops in greater than 90% of carriers. However, reduced penetrance with a large portion of disease-free carrier is seen in some families. Unambiguous identification of the predisposing mutation in these families is important for accurate risk prediction in relatives and their genetic counseling but also provides conceptual information regarding the relationship between the RB1 genotype and the disease phenotype. In this study we report a novel mutation detected in 10 individuals of an extended family, only three of whom are affected by RB disease. The mutation comprises a 23-basepair (bp) duplication in the first exon of RB1 (c.43_65dup) producing a frameshift in exon 1 and premature chain termination in exon 2. Mutations resulting in premature chain termination classically are associated with high penetrance disease, as message translation may not generate functional product and nonsense mediated RNA decay (NMD) frequently eliminates the mutant transcript. However, appreciable NMD does not follow from the mutation described here and transcript expression in tissue culture cells and translation in vitro reveals that alternative in-frame translation start sites involving Met113 and possibly Met233 are used to generate truncated RB1 products (pRB94 and pRB80), known and suspected to exhibit tumor suppressor activity. These results strongly suggest that modulation of disease penetrance in this family is achieved by internal translation initiation. Our observations provide the first example for rescue of a chain-terminating mutation in RB1 through alternative translation initiation.

  9. Análise da transformada em ondeletas aplicada em sinal geofísico

    OpenAIRE

    Bolzan,Maurício José Alves

    2004-01-01

    É mostrada uma análise em ondeletas aplicada em um sinal geofísico, número de manchas solares. Para isso, é dado uma introdução teórica sobre a Transformada em Ondeletas e sobre séries e Transformada de Fourier. A Transformada em Ondeletas é uma ferramenta matemática de grande utilidade aplicação em sinais não-estacionários.

  10. Do modelo asilar-manicomial ao modelo de reabilitação psicossocial: haverá um lugar para o psicanalista em Saúde Mental?

    Directory of Open Access Journals (Sweden)

    Nancy Greca de Oliveira Carneiro

    2008-06-01

    Full Text Available Da desrazão ao doente mental e ao portador de transtorno mental, embora se operem deslocamentos importantes nas Instituições de Saúde Mental, o sujeito permanece excluído. Discute-se a proposta de uma instituição que possa sustentar a lógica do não todo, do caso a caso, onde não se pode escrever o universal ou formar conjunto. Apresenta-se a psicanálise aplicada ao campo da saúde mental como uma clínica do sujeito, fundada não na diversidade, mas na singularidade de sua relação com seu desejo e seu gozo.

  11. Combate ao niilismo e ao totalitarismo em Camus

    Directory of Open Access Journals (Sweden)

    Emanuel Ricardo Germano

    2013-05-01

    Full Text Available A partir de uma leitura da peça Calígula, elaborada entre 1941 e 1943, procurar-se-á contribuir na restituição das preocupações éticas, políticas e filosóficas em cena no teatro de Albert Camus, analisando o sentido de sua expressão teatral no contexto de dois combates,ao niilismo filosófico e ao totalitarismo político de seu tempo. Observaremos em Calígula um importante registro do amadurecimento das leituras de duas heranças filosóficas relevantes na formação intelectual de Camus, Nietzsche e Heráclito, à luz da experiência do totalitarismo, observando e ressaltando com isso a amplitude de seu intertexto filosófico e a importância do aporte ético e político de Camus como pensador multifacetado, engajado nos enfrentamentos históricos e solicitações críticas de seu tempo.

  12. Adrenoleucodistrofia cerebral ligada ao x

    OpenAIRE

    Ono,Sergio Eiji

    2013-01-01

    Resumo: OBJETIVO: o padrão-ouro em exames de imagem para avaliar a adrenoleucodistrofia ligada ao X é o escore de Loes, baseado na localização, alterações de sinais e grau de atrofia. Os objetivos deste estudo foram avaliar como os parâmetros do tensor de difusão correlacionam com o escore de Loes e avaliar se poderiam indicar alterações estruturais precoces. PACIENTES E MÉTODOS: as medidas do tensor de difusão foram obtidas em 9 locais pré-estabelecidos da substância branca, bilateralmente, ...

  13. Educaçao ambiental

    OpenAIRE

    Carneiro,Danielle

    2013-01-01

    Resumo: A idéia desse trabalho surgiu no momento em que me inseri na comunidade de Pontal do Sul, envolvida em distintas atividades educativas com o mesmo público. Uma delas refere-se ao ensino público fundamental e outra à atividades de pesquisa e Educação Ambiental (EA) dentro de um projeto de extensão universitária. A EA vem sendo realizada de forma diversificada, baseada em diferentes concepções, fundamentadas em princípios e diretrizes construídos num momento de difusão da Ecologia e de ...

  14. Experimental Model to Study the Role of Retinoblastoma Gene Product (pRb) for Determination of Adipocyte Differentiation.

    Science.gov (United States)

    Popov, B V; Shilo, P S; Zhidkova, O V; Zaichik, A M; Petrov, N S

    2015-06-01

    Using stable constitutive expression of retinoblastoma gene product (pRb) in polypotent mesenchymal 10T1/2 cells we obtained stable cell lines hyperexpressing functionally active or inactive mutant pRb. The cells producing active exogenous pRb demonstrated high sensitivity to adipocyte differentiation inductors, whereas production of inactive form of the exogenous protein suppressed adipocyte differentiation. The obtained lines can serve as the experimental model for studying the role of pRb in determination of adipocyte differentiation.

  15. Effects of shRNA Targeting Survivin on Apoptosis of Human Retinoblastoma Cell Line Hxo-rb44 in vitro

    Institute of Scientific and Technical Information of China (English)

    WANG Guojun; HU Yanhua; LI Pengcheng

    2006-01-01

    In order to construct a recombinant plasmid containing short hairpin RNA (shRNA) targeting survivin and to investigate its effect on survivin expression and cell apoptosis of human retinoblastoma cell line Hxo-rb44 in vitro, RNA interference plasmid pSIRENS that can express shRNA of survivin was designed, constructed, and transfected into human retinoblastoma cell line Hxo-rb44.Survivin and c-Myc expression was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Apoptosis of Hxo-rb44 cells was assayed by Honchest33258 staining and cell growth curve was drawn. The results showed that the oligonucleotide targeting survivin was identified in pSIRENS plasmid. After pSIRENS plasmid transfected, survivin and c-Myc expression in Hxo-rb44 cells was decreased significantly. Apoptotic rate of cells was up-regulated from (3.5±1.29) % to (36.1±19.66) %. The proliferation ability of Hxo-rb44 cells was inhibited. No significant effects on survivin expression and apoptosis of the cells were found when negative control plasmid was transfected. In conclusion, the plasmid containing shRNA targeting survivin was constructed successfully. It could inhibit efficiently the expression of survivin and c-Myc in human retinoblastoma cell Hxo-rb44 in vitro. The inhibition of the expression of c-Myc might be involved in the apoptosis of Hxo-rb44 cells.

  16. Cdh11 acts as a tumor suppressor in a murine retinoblastoma model by facilitating tumor cell death.

    Directory of Open Access Journals (Sweden)

    Mellone N Marchong

    2010-04-01

    Full Text Available CDH11 gene copy number and expression are frequently lost in human retinoblastomas and in retinoblastomas arising in TAg-RB mice. To determine the effect of Cdh11 loss in tumorigenesis, we crossed Cdh11 null mice with TAg-RB mice. Loss of Cdh11 had no gross morphological effect on the developing retina of Cdh11 knockout mice, but led to larger retinal volumes in mice crossed with TAg-RB mice (p = 0.01. Mice null for Cdh11 presented with fewer TAg-positive cells at postnatal day 8 (PND8 (p = 0.01 and had fewer multifocal tumors at PND28 (p = 0.016, compared to mice with normal Cdh11 alleles. However, tumor growth was faster in Cdh11-null mice between PND8 and PND84 (p = 0.003. In tumors of Cdh11-null mice, cell death was decreased 5- to 10-fold (p<0.03 for all markers, while proliferation in vivo remained unaffected (p = 0.121. Activated caspase-3 was significantly decreased and beta-catenin expression increased in Cdh11 knockdown experiments in vitro. These data suggest that Cdh11 displays tumor suppressor properties in vivo and in vitro in murine retinoblastoma through promotion of cell death.

  17. Inhibition of retinoblastoma mRNA degradation through Poly (A involved in the neuroprotective effect of berberine against cerebral ischemia.

    Directory of Open Access Journals (Sweden)

    Yu-Shuang Chai

    Full Text Available Berberine is one kind of isoquinoline alkaloid with anti-apoptotic effects on the neurons suffering ischemia. To address the explanation for these activities, the berberine-induced cell cycle arrest during neurons suffering ischemia/reperfusion had been studied in the present study. According to the in vitro neurons with oxygen-glucose deprivation and in vivo ICR mice with cerebral ischemia/reperfusion, it was found that berberine could protect the mRNA of retinoblastoma (Rb from degradation through its function on the poly(A tail. The prolonged half-life of retinoblastoma 1 (gene of Rb, RB1 mRNA level secures the protein level of retinoblastoma, which facilitates cell cycle arrest of neurons in the process of ischemia/reperfusion and subsequently avoids cells entering in the apoptotic process. The poly(A tail of RB1 mRNA, as a newly identified target of berberine, could help people focus on the interaction between berberine and mRNA to further understand the biological activities and functions of berberine.

  18. Glycosaminoglycans in human retinoblastoma cells: Heparan sulfate, a modulator of the pigment epithelium-derived factor-receptor interactions

    Science.gov (United States)

    Alberdi, Elena M; Weldon, John E; Becerra, S Patricia

    2003-01-01

    Background Pigment epithelium-derived factor (PEDF) has binding affinity for cell-surface receptors in retinoblastoma cells and for glycosaminoglycans. We investigated the effects of glycosaminoglycans on PEDF-receptor interactions. Results 125I-PEDF formed complexes with protease-resistant components of medium conditioned by human retinoblastoma Y-79 cells. Using specific glycosaminoglycan degrading enzymes in spectrophotometric assays and PEDF-affinity chromatography, we detected heparin and heparan sulfate-like glycosaminoglycans in the Y-79 conditioned media, which had binding affinity for PEDF. The Y-79 conditioned media significantly enhanced the binding of 125I-PEDF to Y-79 cell-surface receptors. However, enzymatic and chemical depletion of sulfated glycosaminoglycans from the Y-79 cell cultures by heparitinase and chlorate treatments decreased the degree of 125I-PEDF binding to cell-surface receptors. Conclusions These data indicate that retinoblastoma cells secrete heparin/heparan sulfate with binding affinity for PEDF, which may be important in efficient cell-surface receptor binding. PMID:12625842

  19. Como responder ao momento presente?

    Directory of Open Access Journals (Sweden)

    Maria Filomena Molder

    2013-06-01

    Full Text Available Foi com esta pergunta — já um efeito de um primeiro encontro entre Irene Pimentel e eu própria — que decidimos desafiar colegas, estudantes e funci­onários da nossa Faculdade, FCSH (Faculdade de Ciências Sociais e Huma­nas, de outras Faculdades da Universidade Nova de Lisboa, de outras Uni­versidades e todos os interessados em con­siderar e discutir em comum aquilo que se passava em Portugal e que no anúncio da Jornada de 6 de De­zembro de 2012 se descrevia como um “processo de desmantela­mento social, económico e cultural sem precedentes — pese embora tantas compara­ções, baseadas na premissa da ‘eterna repetição’ — e cujas consequências não param de exceder as previsões dos responsáveis por esse desmantelamento”. Acedendo com todo o empenho e gratidão ao convite que me foi dirigido por Humberto Brito para fazer uma resenha da Jornada a publicar no primeiro número de Forma de Vida (saúdo a revista e o título, decidi-me, no entanto, a pôr de lado a resenha, que sob a forma de “Editorial” será em breve publi­cada no blogue Responder ao Momento Presente, entre­tanto criado, conjuntamente com os textos escritos pelos nossos convidados, com as parti­cipações de pessoas que corresponderam ao nosso apelo e ainda com contri­bui­ções que se alargaram para lá da Jornada; a que se juntará uma gravação em video, também disponível no Youtube. Texto publicado originalmente em Forma de Vida, Lisboa, n.1, fev. 2013. Agrade­cemos à autora por permitir a republicação neste número do Boletim. [N.E.

  20. CT and MRI diagnosis of retinoblastoma%视网膜母细胞瘤的CT与MRI诊断

    Institute of Scientific and Technical Information of China (English)

    张德翻; 郑文龙; 吴爱琴

    2011-01-01

    Objective: To investigate and compare the value of imaging diagnosis of retinoblastoma. Methods: 16 cases of retinoblastoma were collected in the study. There were 11 cases with CT examination, 4 cases with MRI examination, 1 case with CT and MRI examination. The signs of imaging were retrospectively analyzed. Results: CT signs of retinoblastoma mainly demonstrated soft tissue mass with fleck or plaque calcification on posterior part of the eye ring. The rate of calcification of tumors was 92. 9 % ( 13/14). MRI signs mainly appeared soft tissue mass in the eye ring, some cases with lower signal calcifications in the mass, which the rate of calcification was only 40 % ( 2/5) . But MRI was better to display the adjacent lesion of eye ring. Conclusion : CT was superior to other imaging methods in diagnosis of retinoblastoma , calcification within the mass can be considered qualitative diagnosis of retinoblastoma. Imaging examinarion is important in choice of clinical therapy planning and judgmem of prognosis.%目的:探讨CT与MRI对视网膜母细胞瘤的诊断价值.方法:16例视网膜母细胞瘤,11例行CT检查,4例行MRI检查,1例同时行CT和MRI检查.回顾性分析其影像学征像.结果:视网膜母细胞瘤的CT征象主要表现为眼环后份或以后份为主的软组织肿块,伴有斑点及斑块状钙化,钙化显示率达92.9%(13/14).MRI主要表现为眼环内软组织信号肿块,部分病例肿块内可见更低信号影,钙化显示率仅40%(2/5),但MRI对肿瘤侵犯周围结构较敏感.结论:CT与MRI对视网膜母细胞瘤的诊断价值,以CT较具优势,肿块内钙化可以认为是视网膜母细胞瘤定性诊断依据.影像学检查对于临床确定治疗方案和判断预后具有重要临床价值.

  1. Aerosol Observing System (AOS) Handbook

    Energy Technology Data Exchange (ETDEWEB)

    Jefferson, A

    2011-01-17

    The Aerosol Observing System (AOS) is a suite of in situ surface measurements of aerosol optical and cloud-forming properties. The instruments measure aerosol properties that influence the earth’s radiative balance. The primary optical measurements are those of the aerosol scattering and absorption coefficients as a function of particle size and radiation wavelength and cloud condensation nuclei (CCN) measurements as a function of percent supersaturation. Additional measurements include those of the particle number concentration and scattering hygroscopic growth. Aerosol optical measurements are useful for calculating parameters used in radiative forcing calculations such as the aerosol single-scattering albedo, asymmetry parameter, mass scattering efficiency, and hygroscopic growth. CCN measurements are important in cloud microphysical models to predict droplet formation.

  2. O Army Method e o desenvolvimento da Linguística Aplicada nos Estados Unidos The Army Method and the development of Applied Linguistics in the United States

    Directory of Open Access Journals (Sweden)

    José Marcelo Luna

    2012-01-01

    Full Text Available Este artigo deriva de pesquisa historiográfica sobre o ensino de português nos Estados Unidos durante os anos 40, 50 e 60 do século 20. Baseado em fontes primárias, como livros, períódicos e jornais da época, o trabalho apresenta objetivamente a relação do Army Specialized Training Program, que se tornou conhecido como Army Method, com o desenvolvimento da Linguística Aplicada ao ensino de línguas estrangeiras nos Estados Unidos.This article derives from a historiographic research on the teaching of Portuguese in the United States during 1940 through 1960. Based on primary sources like books, periodicals and newspapers of the time, the work presents objectively the relationship between the Army Specialized Training Program, also known as the Army Method, and the development of Applied Linguistics to the teaching of foreign languages in the United States.

  3. Do valor intrínseco e de sua aplicabilidade ao meio ambiente

    Directory of Open Access Journals (Sweden)

    Tânia Ap. Kuhnen

    2004-01-01

    Full Text Available O tema deste estudo é a teoria do valor sagrado da vida e sua aplicabilidade às questões de ética prática, mais especificamente ao meio ambiente. O objetivo geral consiste em apontar os limites da concepção de Dworkin sobre a teoria do sagrado, quando aplicada ao meio ambiente, e a solução que Singer oferece para a atribuição de valor ao mesmo. Justifica-se esse trabalho em decorrência da importância de se discutir, a partir da análise dessas teorias filosóficas, questões vinculadas ao estabelecimento de um princípio ético que, além de englobar, no campo da ação moral, todos os seres sencientes, envolva também o meio ambiente como um todo. Além disso, o tema que aqui se desenvolve é uma contribuição do raciocínio filosófico-teórico para a discussão de problemas relevantes no campo da ética prática. Esse estudo ainda se justifica por abordar a questão da linha moral divisória entre os seres que são ou não incluídos na comunidade moral, o que representa outro problema que concerne à ética. As diferentes formas de classificação não foram capazes de incluir todos os seres vivos no âmbito da comunidade moral. Há sempre exclusões, as quais tendem a diminuir com o surgimento de teorias éticas inovadoras, dentre elas as que serão abordadas a seguir.

  4. Aprendizagem baseada em equipe: uma estratégia de ensino aplicada na área da imaginologia.

    Directory of Open Access Journals (Sweden)

    Rosana Maria Paiva dos Anjos

    2016-10-01

    Full Text Available Introdução: Metodologias ativas de ensino vêm sendo utilizadas na área médica, atendendo ao desafio de contínuo aprendizado, com novas dinâmicas de relação entre o educador e o educando. Na Aprendizagem Baseada em Equipes (ABE, o educador exerce a função de mediador e facilitador do conhecimento, estimulando a autonomia do aluno e o aprendizado em equipes de trabalho. Objetivo: Desenvolver atividades baseadas no ABE, na área de imaginologia, incentivando o aprendizado colaborativo. Método: A ferramenta pedagógica ABE foi aplicada na área de imaginologia, para 102 alunos, do 2o ano do curso de medicina, na graduação. Cada atividade consistiu em disponibilização prévia de material para estudo, de teste individual e em grupo, de caso clínico e de discussão para o consenso das respostas sobre o conteúdo abordado. A avaliação holística das atividades foi feita através da escala de Likert com dez itens, após o término de cada ciclo semestral de atividades. Resultados: Na pesquisa de opinião da metodologia, foram consideradas “avaliações positivas” (respostas “concordo totalmente” e “concordo parcialmente” e “avaliações negativas” (respostas “não concordo parcialmente” e “não concordo totalmente”. A questão menos pontuada foi referente a estudos prévios. Destacaram-se dentre os dez itens avaliados: “ajudou a ganhar mais conhecimento” e “avaliar os meus conhecimentos prévios”. No desempenho durante a ABE, verificou-se que a média da porcentagem da comparação do desempenho individual com o grupo foi de 23,3% na primeira fase do estudo e de 17,6% na segunda. Conclusão: Os resultados obtidos trazem a percepção de melhor apreensão do conhecimento, nos trabalhos em grupos e de uma boa receptividade para a metodologia. O ensino por ABE no ensino médico prepara o futuro profissional para as tomadas de decisões no trabalho “em equipe”, otimizando a resolução de problemas

  5. Geometría aplicada al dibujo de la arquitectura tratamiento pedagógico

    OpenAIRE

    1994-01-01

    Estructuración de las materias de Geometría, Dibujo y Análisis Arquitectónico con un planteamiento pedagógico, para potenciar en la docencia universitaria la asimilación comprensiva, la creatividad aplicada y la autonomía personal, y una finalida ... Plantea estructuración de las tres materias nuevas, vinculadas con la práctica docente, que involucre al alumnado en el proceso desde una perspectiva didáctica con los objetivos de encontrar las claves de estructuración en la propia materia, logr...

  6. Herramientas de manufactura esbelta aplicadas al desarrollo de software con calidad

    OpenAIRE

    2011-01-01

    El artículo presenta una propuesta de herramientas de manufactura esbelta aplicadas al desarrollo de software, dentro del marco de métodos ágiles, en particular el Desarrollo de Software Esbelto; lo que permitirá a equipos de desarrollo obtener una calidad sistémica del software (producto, procesos y personas que intervienen). Se recomienda emplear estas herramientas y evaluarlas constantemente con la aplicación iterativa e incremental del ciclo de calidad propuesto por Deming: planificar, ha...

  7. Econometría básica aplicada con Gretl

    OpenAIRE

    Esteban González, María Victoria; Moral Zuazo, María Paz; Orbe Mandaluniz, Susan; Regúlez Castillo, Marta; Zárraga Alonso, Ainhoa; Zubia Zubiaurre, Marian

    2009-01-01

    Estas notas de clase son de utilidad como material docente, sirviendo de apoyo o complemento, para aquellos alumnos que bien vayan a hacer o hayan seguido alguna asignatura como Introducción a la Econometría (en LE o LADE), Estadística Actuarial: Regresión (LCAF), Econometría aplicada al mercado (LITM). También puede estar indicada para alumnos de las licenciaturas ofrecidas en la Facultad de Ciencias Sociales y de la Comunicación, por ejemplo la Licenciatura de Publicidad y RR.PP. y de algun...

  8. Medidas de frecuencia, asociación e impacto en investigación aplicada

    OpenAIRE

    2008-01-01

    En la Sociedad actual del conocimiento y la información es preciso disponer de herramientas básicas para medir los fenómenos epidemiológicos como la enfermedad, la incapacidad o la siniestralidad laboral. Las formas de medir son instrumentos que se deben conocer y aplicar para planificar y tomar decisiones en Salud Pública y en Salud Laboral. El objetivo de este artículo es dar a conocer las principales medidas de frecuencia, asociación e impacto utilizadas en investigación aplicada, clínica ...

  9. Tecnología y filosofía wiki aplicadas al electromagnetismo computacional

    OpenAIRE

    De la Cruz Calvo, Daniel

    2007-01-01

    Este trabajo de fin de carrera se ha realizado en la Escola Politècnica Superior de Castelldefels (EPSC) en colaboración con el Centre Internacional de Mètodes Numèrics en Enginyeria (CIMNE) con el objetivo de explorar las posibilidades de la tecnología wiki aplicada al ámbito de investigación de CIMNE: el electromagnetismo computacional. De acuerdo con la Wikipedia, “un (o una) wiki es un sitio web colaborativo que puede ser editado por varios usuarios. Los usuarios de una ...

  10. Projecto Rodentia : etologia aplicada na sala de aula do 1º Ciclo

    OpenAIRE

    2008-01-01

    p. 275-279 O Projecto Rodentia, implementado no ano 2006/2007, visa promover a cultura científica em alunos do 1º ciclo do Ensino Básico, familiarizando-os com o Método Científico e desenvolvendo competências conceptuais, processuais e atitudinais, com base na Etologia Aplicada e na reflexão ética sobre o bem-estar animal. Foram instalados em três salas de aula habitats especialmente desenhados, contendo cada um dois ratos de laboratório. Inicialmente orien...

  11. Tendencia de I+D+i en Biomecánica aplicada al deporte

    OpenAIRE

    Ferro Sanchez, Amelia

    2012-01-01

    Análisis de la investigación en el ámbito de la Biomecánica aplicada al deporte. La tendencia de los proyectos en I+D+i de la Biomecánica del Deporte ha cambiado notablemente en las dos últimas décadas. Los investigadores han pasado de aplicar la biomecánica al alto rendimiento deportivo a tratar temas transversales, relacionados en muchos casos con la salud, fisiología, rehabilitación, el control motor, la gestión y, entre otros, el diseño de equipamiento e instalaciones.

  12. Neurociencia computacional aplicada a la liberación de transmisores por exocitosis

    OpenAIRE

    Fernández Peruchena, Carlos María

    2008-01-01

    Falta por incorporar las palabras clave La Tesis presentada, realizada bajo la dirección del Dr. Guillermo Álvarez de Toledo Naranjo, lleva por título Neurocincia computacional aplicada a la liberación de transmisores por exocitosis. El trabajo desarrollado en esta Tesis forma parte de la actividad de investigación sobre secreción celular que se lleva a cabo en el grupo de investigación del Dr. Álvarez de Toledo (CVI-209), dentro del proyecto BFI2001-3199. El grupo de investigación CVI-209...

  13. Investigación básica y aplicada en contabilidad

    OpenAIRE

    García Casella, Carlos Luis, co-dir.

    2004-01-01

    Se aborda la cuestión de la investigación científica en contabilidad, procurando que sea considerada dentro de las ciencias sociales. Esto genera uso del método científico en la tarea de investigación contable. El carácter de ciencia factual, cultural, aplicada de la contabilidad se considera esencial frente a normas contables profesionales que la reducen a mera técnica en nuestro país. Descartada la idea de arte o tecnología social se presenta un enfoque de contabilidad que supera lo que hab...

  14. Estrategia de comunicación y marketing aplicada a la empresa Nelybel

    OpenAIRE

    García Martín, Beatriz

    2012-01-01

    El objetivo principal del proyecto es plantear una estrategia de comunicación y marketing útil para aplicarla a la empresa de regalos Nelybel. Los objetivos secundarios son aprender a llevar a cabo una estrategia de comunicación y marketing partiendo casi de cero, realizar una investigación de mercado provincial y un análisis de la competencia y ayudar con la publicidad y promoción de la empresa. García Martín, B. (2012). Estrategia de comunicación y marketing aplicada a la empresa Nely...

  15. Emergence of corn according to the sowing depth of the seed and loads on press wheels Emergência do milho conforme profundidade de deposição de sementes e carga aplicada nas rodas compactadoras

    Directory of Open Access Journals (Sweden)

    Pedro H. Weirich Neto

    2012-04-01

    Full Text Available The seeding process was the operation that suffered the most changes in no-tillage system due the cover crop soil and new particle soil arrangement. The objective of this study was to verify the effects of loads applied to the wheels and adjustments of sowing depth on seedling emergence of corn in no-tillage system. The experimental design was completely randomized with a factorial arrangement 5x4, with five loads applied to the wheels and four theoretical sowing depth adjustments. The real sowing depth increased in the lower theoretical depth and decreased in the higher theoretical depth, due to the compaction loads. Regarding the time of emergence, loads applied had not influence at the greater depths. Emergence time decreased with the load increase in the lower depths. Thus, the adjustment of the compactor wheels can influence in the corn seeding process.Devido à presença da cobertura vegetal e ao novo arranjo das partículas do solo no sistema de plantio direto (SPD, o processo de semeadura foi a operação que sofreu as maiores transformações. Sendo assim, objetivou-se verificar o efeito de cargas aplicadas nas rodas compactadoras e de regulagens da profundidade na semeadura de milho em SPD. O delineamento utilizado foi o inteiramente aleatorizado, com arranjo fatorial 5x4, com cinco cargas aplicadas nas rodas compactadoras e quatro regulagens de profundidades teóricas de deposição de sementes. A profundidade real de deposição de sementes aumentou na menor profundidade teórica e diminuiu na maior profundidade teórica, conforme aumento das cargas de compactação. Quanto ao tempo de emergência, nas maiores profundidades teóricas, não houve influência da carga aplicada. Para as menores profundidades, o tempo de emergência diminui com o aumento da carga. Assim, a regulagem das rodas compactadoras pode influenciar no processo de semeadura do milho.

  16. La investigación en pedagogía y didáctica aplicada a la educación física A pesquisa em pedagogia e didática aplicada à educação física Research on Teaching and Didactics Applied to Physical Education

    Directory of Open Access Journals (Sweden)

    Julia Adriana Castro-Carvajal

    2012-12-01

    Full Text Available Este texto forma parte de un proyecto de investigación que busca elaborar un estado del arte sobre investigación educativa en educación física, recreación y deporte en Antioquia entre 1998-2009. Este artículo analiza la producción de conocimiento sobre pedagogía y didáctica aplicada en los campos mencionados. Se trata de dar cuenta de aquello que atañe a las concepciones y los modelos pedagógicos y a los enfoques didácticos hallados en las investigaciones educativas revisadas. Los resultados reflejan la coexistencia de una multiplicidad de enfoques teórico-metodológicos desamparados de un debate pedagógico que, a su vez, reflejan un vacío científico en la comunidad académica.Este texto faz parte de um projeto de pesquisa que pretende elaborar um estado da arte sobre pesquisa educativa em educação física, recreação e esporte em Antioquia entre 1998-2009. Este artigo analisa a produção de conhecimento sobre pedagogia e didática aplicada nos campos mencionados. Trata-se de dar conta daquilo que se refere às concepções, aos modelos pedagógicos e aos enfoques didáticos encontrados nas pesquisas educativas revisadas. Os resultados refletem a coexistência de uma multiplicidade de enfoques teórico-metodológicos desamparados de um debate pedagógico que, por sua vez, refletem um vazio científico na comunidade acadêmica.This article is part of a research project designed to compile information on the state of the art in educational research on physical education, recreation and sports in Antioquia between 1998 and 2009. Specifically, it analyzes the production of knowledge on teaching and didactics applied to the aforementioned fields and, in that respect, it attempts to identify what concerns the pedagogic concepts and models, and the didactic approaches found in the educational research reviewed for the study. The findings point to the coexistence of a wealth of theoretical and methodological approaches bereft of

  17. Clinical presentation and treatment outcome of retinoblastoma in children of South Western China.

    Science.gov (United States)

    Gao, Jingge; Zeng, Jihong; Guo, Bo; He, Weimin; Chen, Jun; Lu, Fang; Chen, Danian

    2016-10-01

    To study the clinical presentation and treatment outcome among children in South Western China with retinoblastoma (RB) and to determine factors predictive of poor outcome.A retrospective review of children diagnosed with RB from 2006 to 2015 at West China Hospital was undertaken. Demographic and clinical characteristics and treatment outcomes were studied.A total of 253 patients (unilateral 80.2%, bilateral 19.8%) were studied. Twenty six patients (10.3%) were from minority ethnic groups of China. The median onset age was 21 months. Leukocoria was the most common presenting sign (71%). Tumors were intraocular in 91.3% cases, extraocular in 8.7% cases. Extraocular RB patients had a longer median lag period than intraocular patients (9 months vs 2 months, P presentation is related to extraocular RB which is a risk factor for poor outcome. Chemotherapy increased the eye salvage but had no effects to overall survival. Education for parents and general physicians for the early signs of RB (such as leukocoria), therapeutic strategy and treatment outcomes of RB may promote early diagnosis, improve the compliance, and outcome.

  18. Interaction of fish aryl hydrocarbon receptor paralogs (AHR1 and AHR2) with the retinoblastoma protein

    Energy Technology Data Exchange (ETDEWEB)

    Merson, Rebeka R., E-mail: rmerson@ric.edu [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Biology Department, Rhode Island College, 500 Mt. Pleasant Ave., Providence, RI 02908 (United States); Karchner, Sibel I.; Hahn, Mark E. [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States)

    2009-08-13

    The aryl hydrocarbon receptor (AHR) mediates the toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related compounds. In some mammalian cell lines, TCDD induces G1 cell cycle arrest, which depends on an interaction between the AHR and the retinoblastoma tumor suppressor (RB). Mammals possess one AHR, whereas fishes possess two or more AHR paralogs that differ in the domains important for AHR-RB interactions in mammals. To test the hypothesis that fish AHR paralogs differ in their ability to interact with RB, we cloned RB cDNA from Atlantic killifish, Fundulus heteroclitus, and studied the interactions of killifish RB protein with killifish AHR1 and AHR2. In coimmunoprecipitation experiments, in vitro-expressed killifish RB coprecipitated with both AHR1 and AHR2. Consistent with these results, both killifish AHR1 and AHR2 interacted with RB in mammalian two-hybrid assays. These results suggest that both fish AHR1 and AHR2 paralogs may have the potential to influence cell proliferation through interactions with RB.

  19. Proton and electron deep dose profiles for retinoblastoma based on GEANT 4 code

    Energy Technology Data Exchange (ETDEWEB)

    Braga, Flavia V., E-mail: flaviafisica@gmail.co [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Programa de Pos-graduacao em Ciencias e Tecnicas Nucleares; Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Campos, Tarcisio P.R. de [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Programa de Pos-graduacao em Ciencias e Tecnicas Nucleares; Ribeiro, Kilder L., E-mail: kilderlr@gmail.co [Universidade Estadual de Feira de Santana (UEFS), BA (Brazil). Dept. de Fisica

    2009-07-01

    Herein, the dosimetry responses to a retinoblastoma proton and electron radiation therapy were investigated. The computational tool applied to this simulation was the Geant4 code, version 4.9.1. The code allows simulating the charge particle interaction with eyeball tissue. In the present simulation, a box of 4 cm side water filled had represented the human eye. The simulation was performed considering mono energetic beams of protons and electrons with spectra of 57 to 70 MeV for protons and 2 to 8 MeV for electrons. The simulation was guide by the advanced hadron therapy example distributed with the Geant4 code. The phantom was divided in voxels with 0.2 mm side. The energy deposited in each voxel was evaluated taken the direct beam at one face. The simulation results show the delivery energy and therefore the dose deposited in each voxel. The deep dose profiles to proton and electron were plotted. The well known Bragg peak was reproduced for protons. The maximum delivered dose defined the position at the proton stopped. However, to electrons, the absorbed energies were delivered along its path producing a more continuous distribution following the water depth, but also being stopped in the end of its path. (author)

  20. Structures of inactive retinoblastoma protein reveal multiple mechanisms for cell cycle control

    Energy Technology Data Exchange (ETDEWEB)

    Burke, Jason R.; Hura, Greg L.; Rubin, Seth M. (UCSC); (LBNL)

    2012-07-18

    Cyclin-dependent kinase (Cdk) phosphorylation of the Retinoblastoma protein (Rb) drives cell proliferation through inhibition of Rb complexes with E2F transcription factors and other regulatory proteins. We present the first structures of phosphorylated Rb that reveal the mechanism of its inactivation. S608 phosphorylation orders a flexible 'pocket' domain loop such that it mimics and directly blocks E2F transactivation domain (E2F{sup TD}) binding. T373 phosphorylation induces a global conformational change that associates the pocket and N-terminal domains (RbN). This first multidomain Rb structure demonstrates a novel role for RbN in allosterically inhibiting the E2F{sup TD}-pocket association and protein binding to the pocket 'LxCxE' site. Together, these structures detail the regulatory mechanism for a canonical growth-repressive complex and provide a novel example of how multisite Cdk phosphorylation induces diverse structural changes to influence cell cycle signaling.

  1. Retinoblastoma diagnosis: a proposal based on the experience of Centro Infantil Boldrini, Brazil.

    Science.gov (United States)

    Palazzi, Maristela A; Stephan, Celso; Brandalise, Silvia R; Aguiar, Simone Dos Santos

    2013-08-01

    Advanced disease is a risk factor for eye loss in patients with retinoblastoma (RB). We still record critical rates of enucleation, especially for unilateral RB due to advanced stages of disease at diagnosis. This retrospective study of 223 RB patient records referred to treatment at Centro Infantil Boldrini, Brazil, between 1978 and 2008, showed that 176 patients (79%) presented intraocular tumors while 47 (21%) already had extraocular involvement. At the time of diagnosis, the age of patients was 26.2 months in the group that had enucleated eyes and 13.7 months in the group that preserved both eyes. Under a multiple logistic regression model, familial history (OR = 0.195; p = .01) and age at diagnosis in months (OR = 1.047; p = .04) were significantly correlated with enucleation. Strategies to early detect RB must be changed in order to offer better chances of ocular preservation with visual function. Authors propose a systematic referral of all children to the ophthalmologist for an indirect ophthalmoscopy once a year in the first two years of life, as a measure to be adopted by all pediatricians in daily routine to early detect the tumor.

  2. IGF-2 is necessary for retinoblastoma-mediated enhanced adaptation after small-bowel resection.

    Science.gov (United States)

    Choi, Pamela M; Sun, Raphael C; Sommovilla, Josh; Diaz-Miron, Jose; Guo, Jun; Erwin, Christopher R; Warner, Brad W

    2014-11-01

    Previously, we have demonstrated that genetically disrupting retinoblastoma protein (Rb) expression in enterocytes results in taller villi, mimicking resection-induced adaption responses. Rb deficiency also results in elevated insulin-like growth factor-2 (IGF-2) expression in villus enterocytes. We propose that postoperative disruption of Rb results in enhanced adaptation which is driven by IGF-2. Inducible, intestine-specific Rb-null mice (iRbIKO) and wild-type (WT) littermates underwent a 50% proximal small-bowel resection (SBR) at 7-9 weeks of age. They were then given tamoxifen on postoperative days (PODs) 4-6 and harvested on POD 28. The experiment was then repeated on double knockouts of both IGF-2 and Rb (IGF-2 null/iRbIKO). iRbIKO mice demonstrated enhanced resection-induced adaptive villus growth after SBR and increased IGF-2 messenger RNA (mRNA) in ileal villus enterocytes compared to their WT littermates. In the IGF-2 null/iRbIKO double-knockout mice, there was no additional villus growth beyond what was expected of normal resection-induced adaptation. Adult mice in which Rb is inducibly deleted from the intestinal epithelium following SBR have augmented adaptive growth. IGF-2 expression is necessary for enhanced adaptation associated with acute intestinal Rb deficiency.

  3. Inhibition of proliferation and induction of apoptosis in RB116 retinoblastoma cells by afatinib treatment.

    Science.gov (United States)

    Zhan, Wei-Jiao; Zhu, Jian-Feng; Wang, Long-Mei

    2016-07-01

    The present study investigates the effect of afatinib on the growth, induction of apoptosis in RB116 cells, and reduction of carcinoma growth in the mice transplanted with RB116 cells. The results from MTT assay revealed that afatinib inhibited the growth of RB116 cells in a dose-dependent manner. Proliferation of RB116 cells was reduced to 64 % on treatment with 200 μM concentration of afatinib after 48 h. Afatinib treatment of RB116 cells at 200 μM concentration induced apoptosis and necrosis in 49.7 and 9.4 %, respectively, after 48 h. In the RB116-transplanted mice, treatment with afatinib at 10-mg/kg doses for 45 days caused a significant (p afatinib treatment group after 45 days. However, the expression of caspase-3 was increased and of Bcl-2 remained unaltered on treatment with afatinib. Measurement of the body weight of afatinib-treated animals showed no reduction during the study. Thus, afatinib can be of therapeutic value for the treatment of retinoblastoma.

  4. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Scheffner, M.; Muenger, K.; Byrne, J.C.; Howley, P.M. (National Cancer Inst., Bethesda, MD (United States))

    1991-07-01

    Human cervical carcinoma cell lines that were either positive or negative for human papillomavirus (HPV) DNA sequences were analyzed for evidence of mutation of the p53 and retinoblastoma genes. Each of five HPV-positive cervical cancer cell lines expressed normal pRB and low levels of wild-type p53 proteins, which are presumed to be altered in function as a consequence of association with HPV E7 and E6 oncoproteins, respectively. In contrast, mutations were identified in the p53 and RB genes expressed in the C-33A and HT-3 cervical cancer cell lines, which lack HPV DNA sequences. Mutations in the p53 genes mapped to codon 273 and codon 245 in the C33-A and HT-3 cell lines, respectively, located in the highly conserved regions of p53, where mutations appear in a variety of human cancers. Mutations in RB occurred at splice junctions, resulting in in-frame deletions, affecting exons 13 and 20 in the HT-3 and C-33A cell lines, respectively. These mutations resulted in aberrant proteins that were not phosphorylated and were unable to complex with the adenovirus E1A oncoprotein. These results support the hypothesis that the inactivation of the normal functions of the tumor-suppressor proteins pRB and p53 are important steps in human cervical carcinogenesis, either by mutation or from complex formation with the HPV E6 and E7 oncoproteins.

  5. Prediction and Analysis of Retinoblastoma Related Genes through Gene Ontology and KEGG

    Directory of Open Access Journals (Sweden)

    Zhen Li

    2013-01-01

    Full Text Available One of the most important and challenging problems in biomedicine is how to predict the cancer related genes. Retinoblastoma (RB is the most common primary intraocular malignancy usually occurring in childhood. Early detection of RB could reduce the morbidity and promote the probability of disease-free survival. Therefore, it is of great importance to identify RB genes. In this study, we developed a computational method to predict RB related genes based on Dagging, with the maximum relevance minimum redundancy (mRMR method followed by incremental feature selection (IFS. 119 RB genes were compiled from two previous RB related studies, while 5,500 non-RB genes were randomly selected from Ensemble genes. Ten datasets were constructed based on all these RB and non-RB genes. Each gene was encoded with a 13,126-dimensional vector including 12,887 Gene Ontology enrichment scores and 239 KEGG enrichment scores. Finally, an optimal feature set including 1061 GO terms and 8 KEGG pathways was obtained. Analysis showed that these features were closely related to RB. It is anticipated that the method can be applied to predict the other cancer related genes as well.

  6. Estudo do raspado de conjuntiva de pacientes tratados de retinoblastoma: Etapa II ¾ citologia

    Directory of Open Access Journals (Sweden)

    Santos Maria Carmen Menezes

    2002-01-01

    Full Text Available Objetivo: A citologia da conjuntiva humana tem sido amplamente estu-dada. A falta de dados sobre as características do raspado ocular em pacientes tratados de retinoblastoma submetidos à enucleação e radioterapia foi o que motivou este estudo. Métodos: Foram examinadas 114 órbitas de 58 pacientes. Este trabalho apresenta os resultados da citologia conjuntival, em 4 condições, com e sem enucleação, com e sem radioterapia. Resultados: No infiltrado leucocitário das órbitas com bulbo ocular predominaram os linfócitos e nas demais, os polimorfonucleares neutrófilos. De maneira geral, as alterações das células epiteliais mais encontradas nas lâminas dos esfregaços conjuntivais de órbitas irradiadas foram: células com núcleo aumentado e nucléolo proeminente e com hialinização do citoplasma. Conclusão: Os achados citológicos do raspado conjuntival não indicaram perfil característico e distinto para cada grupo.

  7. Direct Involvement of Retinoblastoma Family Proteins in DNA Repair by Non-homologous End-Joining

    Directory of Open Access Journals (Sweden)

    Rebecca Cook

    2015-03-01

    Full Text Available Deficiencies in DNA double-strand break (DSB repair lead to genetic instability, a recognized cause of cancer initiation and evolution. We report that the retinoblastoma tumor suppressor protein (RB1 is required for DNA DSB repair by canonical non-homologous end-joining (cNHEJ. Support of cNHEJ involves a mechanism independent of RB1’s cell-cycle function and depends on its amino terminal domain with which it binds to NHEJ components XRCC5 and XRCC6. Cells with engineered loss of RB family function as well as cancer-derived cells with mutational RB1 loss show substantially reduced levels of cNHEJ. RB1 variants disabled for the interaction with XRCC5 and XRCC6, including a cancer-associated variant, are unable to support cNHEJ despite being able to confer cell-cycle control. Our data identify RB1 loss as a candidate driver of structural genomic instability and a causative factor for cancer somatic heterogeneity and evolution.

  8. Retinoblastoma Protein Plays Multiple Essential Roles in the Terminal Differentiation of Sertoli Cells

    Science.gov (United States)

    Nalam, Roopa L.; Andreu-Vieyra, Claudia; Braun, Robert E.; Akiyama, Haruhiko; Matzuk, Martin M.

    2009-01-01

    Retinoblastoma protein (RB) plays crucial roles in cell cycle control and cellular differentiation. Specifically, RB impairs the G1 to S phase transition by acting as a repressor of the E2F family of transcriptional activators while also contributing towards terminal differentiation by modulating the activity of tissue-specific transcription factors. To examine the role of RB in Sertoli cells, the androgen-dependant somatic support cell of the testis, we created a Sertoli cell-specific conditional knockout of Rb. Initially, loss of RB has no gross effect on Sertoli cell function because the mice are fertile with normal testis weights at 6 wk of age. However, by 10–14 wk of age, mutant mice demonstrate severe Sertoli cell dysfunction and infertility. We show that mutant mature Sertoli cells continue cycling with defective regulation of multiple E2F1- and androgen-regulated genes and concurrent activation of apoptotic and p53-regulated genes. The most striking defects in mature Sertoli cell function are increased permeability of the blood-testis barrier, impaired tissue remodeling, and defective germ cell-Sertoli cell interactions. Our results demonstrate that RB is essential for proper terminal differentiation of Sertoli cells. PMID:19819985

  9. Absence of inhibin alpha and retinoblastoma protein leads to early sertoli cell dysfunction.

    Directory of Open Access Journals (Sweden)

    Roopa L Nalam

    Full Text Available Sertoli cells, the support cells of mammalian spermatogenesis, are regulated by a number of nuclear factors and express retinoblastoma (RB tumor suppressor protein. We hypothesized that RB is an important mediator of Sertoli cell tumorigenesis in inhibin alpha knockout (Inha KO mice. In our previous mouse studies, we found that conditional knockout (cKO of Rb in Sertoli cells caused progressive Sertoli cell dysfunction. Initially, loss of RB had no gross effect on Sertoli cell function as the mice were fertile with normal testis weights at 6 weeks of age, but by 10-14 weeks of age, mutant mice demonstrated severe Sertoli cell dysfunction and infertility. Although double knockout (dKO of Rb and Inha did not result in exacerbation of the tumorigenic phenotype of Inha-null mice, we found that the dKO mice demonstrate an acceleration of Sertoli cell dysfunction compared to Rb cKO mice. Specifically, in contrast to Rb cKO mice, Inha/Rb dKO mice showed signs of Sertoli cell dysfunction as early as 4 weeks of age. These results demonstrate that RB is not essential for Sertoli cell tumorigenesis in Inha KO mice but that loss of Inha accelerates the infertility phenotype of Rb cKO mice.

  10. Absence of Inhibin Alpha and Retinoblastoma Protein Leads to Early Sertoli Cell Dysfunction

    Science.gov (United States)

    Nalam, Roopa L.; Andreu-Vieyra, Claudia; Matzuk, Martin M.

    2010-01-01

    Sertoli cells, the support cells of mammalian spermatogenesis, are regulated by a number of nuclear factors and express retinoblastoma (RB) tumor suppressor protein. We hypothesized that RB is an important mediator of Sertoli cell tumorigenesis in inhibin α knockout (Inha KO) mice. In our previous mouse studies, we found that conditional knockout (cKO) of Rb in Sertoli cells caused progressive Sertoli cell dysfunction. Initially, loss of RB had no gross effect on Sertoli cell function as the mice were fertile with normal testis weights at 6 weeks of age, but by 10–14 weeks of age, mutant mice demonstrated severe Sertoli cell dysfunction and infertility. Although double knockout (dKO) of Rb and Inha did not result in exacerbation of the tumorigenic phenotype of Inha-null mice, we found that the dKO mice demonstrate an acceleration of Sertoli cell dysfunction compared to Rb cKO mice. Specifically, in contrast to Rb cKO mice, Inha/Rb dKO mice showed signs of Sertoli cell dysfunction as early as 4 weeks of age. These results demonstrate that RB is not essential for Sertoli cell tumorigenesis in Inha KO mice but that loss of Inha accelerates the infertility phenotype of Rb cKO mice. PMID:20676395

  11. PCR detection of retinoblastoma gene deletions in radiation-induced mouse lung adenocarcinomas

    Energy Technology Data Exchange (ETDEWEB)

    Churchill, M.E.; Gemmell, M.A.; Woloschak, G.E.

    1994-05-01

    From 1971--1986, Argonne National Laboratory conducted a series of large-scale studies of tumor incidence in 40,000 BCF{sub 1} mice irradiated with {sup 60}Co {gamma}-rays or JANUS fission-spectrum neutrons. Polymerase chain reaction (PCR) technique was used to detect deletions in the mouse retinoblastoma (mRb) gene. Six mRb gene exon fragments were amplified in a 40-cycle, 3-temperature PCR protocol. Absence of any of these fragments on a Southern blot indicated a deletion of that portion of the mRb gene. Tumors chosen for analysis were lung adenocarcinomas that were judged to be the cause of death in post-mortem analyses. Spontaneous tumors as well as those from irradiated mice were analyzed for mRb deletions. In all normal mouse tissues studies all six mRb exon fragments were present on Southern blots. Tumors in six neutron-irradiated mice also had no mRb deletions. However, 1 of 6 tumors from {gamma}-irradiated mice and 6 of 18 spontaneous tumors from unirradiated mice showed a deletion in one or both mRb alleles. All deletions detected were in the 5{prime} region of the mRb gene.

  12. Mutational screening of the RB1 gene in Italian patients with retinoblastoma reveals 11 novel mutations.

    Science.gov (United States)

    Sampieri, Katia; Hadjistilianou, Theodora; Mari, Francesca; Speciale, Caterina; Mencarelli, Maria Antonietta; Cetta, Francesco; Manoukian, Siranoush; Peissel, Bernard; Giachino, Daniela; Pasini, Barbara; Acquaviva, Antonio; Caporossi, Aldo; Frezzotti, Renato; Renieri, Alessandra; Bruttini, Mirella

    2006-01-01

    Retinoblastoma (RB, OMIM#180200) is the most common intraocular tumour in infancy and early childhood. Constituent mutations in the RB1 gene predispose individuals to RB development. We performed a mutational screening of the RB1 gene in Italian patients affected by RB referred to the Medical Genetics of the University of Siena. In 35 unrelated patients, we identified germline RB1 mutations in 6 out of 9 familial cases (66%) and in 7 out of 26 with no family history of RB (27%). Using the single-strand conformational polymorphism (SSCP) technique, 11 novel mutations were detected, including 3 nonsense, 5 frameshift and 4 splice-site mutations. Only two of these mutations (1 splice site and 1 missense) were previously reported. The mutation spectrum reflects the published literature, encompassing predominately nonsense or frameshift and splicing mutations. RB1 germline mutation was detected in 37% of our cases. Gross rearrangements outside the investigated region, altered DNA methylation, or mutations in non-coding regions, may be the cause of disease in the remainder of the patients. Some cases, e.g. a case of incomplete penetrance, or variable expressivity ranging from retinoma to multiple tumours, are discussed in detail. In addition, a case of pre-conception genetic counselling resolved by rescue of banked cordonal blood of the affected deceased child is described.

  13. Expression of altered retinoblastoma protein inversely correlates with tumor invasion in gastric carcinoma

    Institute of Scientific and Technical Information of China (English)

    Nan-Hua Chou; Hui-Chun Chen; Nan-Song Chou; Ping-I Hsu; Hui-Hwa Tseng

    2006-01-01

    AIM: To investigate the clinical and pathological significance of altered retinoblastoma (Rb) encoding protein (pRb) in gastric carcinoma.METHODS: Expression of altered pRb was analyzed in 91 patients with gastric adenocarcinoma by immunohistochemistry.RESULTS: Sixty-five percent (59/91) of the tumors were positively stained and the staining in tumor nuclei of gastric carcinoma ranged 0%-90%. Moreover, strong expression of altered pRb was found in 35% (6/17),24% (5/21), 17% (8/46) and 0% (0/7) of T1, T2, T3 and T4 gastric carcinomas, respectively. Altered pRb expression was inversely correlated with the depth of tumor invasion (P = 0.047). Degree of immunoreactivity had no significant correlation with tumor grade, node metastasis and distant metastasis. In terms of prognostic significance, univariate analysis showed that poor differentiation [41 (66.1%) vs 34 (42.5%) P = 0.051],advanced tumor stage (P < 0.001) and weakly altered pRb expression [17 (80.5%) vs 58 (49.6%) P = 0.044]were associated with worse prognosis in these patients.However, multivariate analysis revealed that advanced tumor stage was the only independent poor prognostic factor (P < 0.001).CONCLUSION: The mutation of Rb gene is frequent in gastric carcinoma. The expression of altered pRb inversely correlates with tumor invasion and is not an independent prognostic marker in gastric adenocarcinoma

  14. Nutlin-3 down-regulates retinoblastoma protein expression and inhibits muscle cell differentiation

    Energy Technology Data Exchange (ETDEWEB)

    Walsh, Erica M. [Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118 (United States); Niu, MengMeng; Bergholz, Johann [Center of Growth, Metabolism and Aging, College of Life Sciences, Sichuan University, Chengdu, 610014 China (China); Jim Xiao, Zhi-Xiong, E-mail: jxiao@bu.edu [Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118 (United States); Center of Growth, Metabolism and Aging, College of Life Sciences, Sichuan University, Chengdu, 610014 China (China)

    2015-05-29

    The p53 tumor suppressor gene plays a critical role in regulation of proliferation, cell death and differentiation. The MDM2 oncoprotein is a major negative regulator for p53 by binding to and targeting p53 for proteasome-mediated degradation. The small molecule inhibitor, nutlin-3, disrupts MDM2-p53 interaction resulting in stabilization and activation of p53 protein. We have previously shown that nutlin-3 activates p53, leading to MDM2 accumulation as concomitant of reduced retinoblastoma (Rb) protein stability. It is well known that Rb is important in muscle development and myoblast differentiation and that rhabdomyosarcoma (RMS), or cancer of the skeletal muscle, typically harbors MDM2 amplification. In this study, we show that nutlin-3 inhibited myoblast proliferation and effectively prevented myoblast differentiation, as evidenced by lack of expression of muscle differentiation markers including myogenin and myosin heavy chain (MyHC), as well as a failure to form multinucleated myotubes, which were associated with dramatic increases in MDM2 expression and decrease in Rb protein levels. These results indicate that nutlin-3 can effectively inhibit muscle cell differentiation. - Highlights: • Nutlin-3 inhibits myoblast proliferation and prevents differentiation into myotubes. • Nutlin-3 increases MDM2 expression and down-regulates Rb protein levels. • This study has implication in nutlin-3 treatment of rhabdomyosarcomas.

  15. Structural insights into the mechanism of phosphoregulation of the retinoblastoma protein.

    Directory of Open Access Journals (Sweden)

    Ekaterina P Lamber

    Full Text Available The retinoblastoma susceptibility protein RB1 is a key regulator of cell proliferation and fate. RB1 operates through nucleating the formation of multi-component protein complexes involved in the regulation of gene transcription, chromatin structure and protein stability. Phosphorylation of RB1 by cyclin-dependent kinases leads to conformational alterations and inactivates the capability of RB1 to bind partner protein. Using small angle X-ray scattering in combination with single particle analysis of transmission electron microscope images of negative-stained material we present the first three-dimensional reconstruction of non-phosphorylated RB1 revealing an extended architecture and deduce the domain arrangement within the molecule. Phosphorylation results in an overt alteration of the molecular shape and dimensions, consistent with the transition to a compact globular architecture. The work presented provides what is to our knowledge the first description of the relative domain arrangement in active RB1 and predicts the molecular movement that leads to RB1 inactivation following protein phosphorylation.

  16. Overview of recurrent chromosomal losses in retinoblastoma detected by low coverage next generation sequencing

    Science.gov (United States)

    García-Chequer, A.J.; Méndez-Tenorio, A.; Olguín-Ruiz, G.; Sánchez-Vallejo, C.; Isa, P.; Arias, C.F.; Torres, J.; Hernández-Angeles, A.; Ramírez-Ortiz, M.A.; Lara, C.; Cabrera-Muñoz, M.L.; Sadowinski-Pine, S.; Bravo-Ortiz, J.C.; Ramón-García, G.; Diegopérez-Ramírez, J.; Ramírez-Reyes, G.; Casarrubias-Islas, R.; Ramírez, J.; Orjuela, M.A.; Ponce-Castañeda, M.V.

    2016-01-01

    Genes are frequently lost or gained in malignant tumors and the analysis of these changes can be informative about the underlying tumor biology. Retinoblastoma is a pediatric intraocular malignancy, and since deletions in chromosome 13 have been described in this tumor, we performed genome wide sequencing with the Illumina platform to test whether recurrent losses could be detected in low coverage data from DNA pools of Rb cases. An in silico reference profile for each pool was created from the human genome sequence GRCh37p5; a chromosome integrity score and a graphics 40 Kb window analysis approach, allowed us to identify with high resolution previously reported non random recurrent losses in all chromosomes of these tumors. We also found a pattern of gains and losses associated to clear and dark cytogenetic bands respectively. We further analyze a pool of medulloblastoma and found a more stable genomic profile and previously reported losses in this tumor. This approach facilitates identification of recurrent deletions from many patients that may be biological relevant for tumor development. PMID:26883451

  17. Modulation of multidrug resistance 1 expression and function in retinoblastoma cells by curcumin

    Directory of Open Access Journals (Sweden)

    Seethalakshmi Sreenivasan

    2013-01-01

    Full Text Available Objective: To determine the possible interaction of curcumin with P-glycoprotein (P-gp expression and function by in vitro and in silico studies. Materials and Methods: In this study, curcumin was compared for its potential to modulate the expression and function of P-gp in Y79 RB cells by western blot, RT-PCR (reverse transcription polymerase chain reaction and functional assay. Further, in silico molecular modeling and docking simulations were performed to deduce the inhibitory binding mode of curcumin. Results: Western blot and RT-PCR analysis decreased the expression of P-gp in a dose-dependent manner. The effect of curcumin on P-gp function was demonstrated by Rhodamine 123 (Rh123 accumulation and efflux study. Curcumin increased the accumulation of Rh123 and decreased its efflux in retinoblastoma (RB cells. In addition, curcumin inhibited verapamil stimulated ATPase activity and photoaffinity labeling study showed no effect on the binding of 8-azido-ATP-biotin, indicating its interaction at the substrate binding site. Moreover, molecular docking studies concurrently infer the binding of curcumin into the substrate binding site of P-gp with a binding energy of -7.66 kcal/mol. Conclusion: These findings indicate that curcumin suppresses the MDR1 expression and function, and therefore may be useful as modulators of multidrug resistance in RB tumor.

  18. Uptake of prenatal diagnostic testing for retinoblastoma compared to other hereditary cancer syndromes in the Netherlands.

    Science.gov (United States)

    Dommering, Charlotte J; Henneman, Lidewij; van der Hout, Annemarie H; Jonker, Marianne A; Tops, Carli M J; van den Ouweland, Ans M W; van der Luijt, Rob B; Mensenkamp, Arjen R; Hogervorst, Frans B L; Redeker, Egbert J W; de Die-Smulders, Christine E M; Moll, Annette C; Meijers-Heijboer, Hanne

    2017-04-01

    Since the 1980s the genetic cause of many hereditary tumor syndromes has been elucidated. As a consequence, carriers of a deleterious mutation in these genes may opt for prenatal diagnoses (PND). We studied the uptake of prenatal diagnosis for five hereditary cancer syndromes in the Netherlands. Uptake for retinoblastoma (Rb) was compared with uptake for Von Hippel-Lindau disease (VHL), Li-Fraumeni syndrome (LFS), familial adenomatous polyposis (FAP), and hereditary breast ovarian cancer (HBOC). A questionnaire was completed by all nine DNA-diagnostic laboratories assessing the number of independent mutation-positive families identified from the start of diagnostic testing until May 2013, and the number of PNDs performed for these syndromes within these families. Of 187 families with a known Rb-gene mutation, 22 had performed PND (11.8%), this was significantly higher than uptake for FAP (1.6%) and HBOC (<0.2%). For VHL (6.5%) and LFS (4.9%) the difference was not statistically significant. PND for Rb started 3 years after introduction of diagnostic DNA testing and remained stable over the years. For the other cancer syndromes PND started 10-15 years after the introduction and uptake for PND showed an increase after 2009. We conclude that uptake of PND for Rb was significantly higher than for FAP and HBOC, but not different from VHL and LFS. Early onset, high penetrance, lack of preventive surgery and perceived burden of disease may explain these differences.

  19. RBAP96 Mediates Radiosensitivity of Breast Cancer Cells via Interacting with Retinoblastoma Protein

    Institute of Scientific and Technical Information of China (English)

    Junling Zhang; Xiaolei Xue; Qinghui Meng; Lu Lu; Ming Cui; Saijun Fan

    2016-01-01

    Objective To identify a novel retinoblastoma protein(RB)-associated protein(RBAP 96)and to explore the impact of RBAP96 on radiosensitivity of human breast cancer cells.Methods An in vivo and in vitro association of RBAP96 with RB was determined by immunoprecipitation-Western blotting and GST pull-down assay.Protein expression was measured by Western blot assay.Cellular survival was evaluated by using a colony formation assay.Results In both in vitro and in vivo assays,we found that the RBAP96 and RB interaction required a 513LXCXE517 motif on the RBAP96 protein and an intact A/B binding pocket of RB.RBAP96 enhances RB-mediated transcriptional repression.Finally,enforced expression of RBAP96 caused an elevated radiosensitivity of human breast cancer cells bearing wtRB,but did not affect radiosensitivity of breast cancer cells bearing mutant RB.Expression of a full-length RBAP96 with an 513LXCXE517 inactivating mutation(LXCXE→RXRXH) failed to result in any radiosensitivity alteration.Conclusion This study for the first time characterizes a novel RB-interacting protein RBAP96 and demonstrates that enforced expression of RBAP96 causes an increase of RBAP96-mediated transcription activation and radiosensitivity via a RB-interacting dependent manner.

  20. The Promyelocytic Leukemia Gene Product (PML) Forms Stable Complexes with the Retinoblastoma Protein

    Science.gov (United States)

    Alcalay, Myriam; Tomassoni, Lucia; Colombo, Emanuela; Stoldt, Stephan; Grignani, Francesco; Fagioli, Marta; Szekely, Laszlo; Helin, Kristian; Pelicci, Pier Giuseppe

    1998-01-01

    PML is a nuclear protein with growth-suppressive properties originally identified in the context of the PML-retinoic acid receptor α (RARα) fusion protein of acute promyelocytic leukemia. PML localizes within distinct nuclear structures, called nuclear bodies, which are disrupted by the expression of PML-RARα. We report that PML colocalizes with the nonphosphorylated fraction of the retinoblastoma protein (pRB) within nuclear bodies and that pRB is delocalized by PML-RARα expression. Both PML and PML-RARα form complexes with the nonphosphorylated form of pRB in vivo, and they interact with the pocket region of pRB. The regions of PML and PML-RARα involved in pRB binding differ; in fact, the B boxes and the C-terminal region of PML, the latter of which is not present in PML-RARα, are essential for the formation of stable complexes with pRB. Functionally, PML abolishes activation of glucocorticoid receptor-regulated transcription by pRB, whereas PML-RARα further increases it. Our results suggest that PML may be part of transcription-regulatory complexes and that the oncogenic potential of the PML-RARα protein may derive from the alteration of PML-regulated transcription. PMID:9448006

  1. Diretivas antecipadas de vontade aos doentes terminais: revisão integrativa

    Directory of Open Access Journals (Sweden)

    Silvana Bastos Cogo

    2015-06-01

    Full Text Available RESUMOObjetivo:caracterizar a produção científica nacional e internacional sobre as diretivas antecipadas de vontade aplicadas ao doente terminal.Método:a revisão integrativa, incluindo os artigos publicados no Portal Capes, SCIELO, LILACS, MEDLINE, Revista de Bioética e Bioethikos, a partir dos descritores: Diretivas antecipadas, Testamentos quanto à vida, Advance Directives, Living Will e Terminally Ill totalizando 44 artigos submetidos à análise de conteúdo.Resultados:emergiram três categorias: Estudantes e profi ssionais frente às diretivas antecipadas de vontade: percepções, opiniões e condutas; Receptividade dos pacientes às diretivas antecipadas de vontade; A família diante das diretivas antecipadas de vontade.Conclusão:evidenciou-se a relevância do tema como garantidor do respeito à dignidade e à autonomia do doente, bem como para a redução dos conflitos éticos enfrentados pelos familiares e profi ssionais da saúde frente aos cuidados em fi nal de vida.

  2. Fuzzy logic applied to the control of the energy consumption in intelligent buildings; Logica fuzzy aplicada ao controle do consumo de energia eletrica em edificios inteligentes

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Herbert R. do N.

    1998-02-01

    This work shows a study on the using of fuzzy control algorithms for the energy optimization of a standard building. The simulation of this type of control was performed using a central conditioned air model and the fuzzy control architecture already used in various control projects. This situation allowed a comparative study among the the control algorithms normally used in conditioned air installations, and the control performed through the building automation system, using an algorithm based on fuzzy logic.

  3. Computação biologicamente inspirada aplicada ao estudo da interação da monoamina oxidase e inibidores

    OpenAIRE

    Moraes, Fernanda Pretto

    2011-01-01

    A Doença de Parkinson (DP) é uma doença neurológica intrigante que afeta a população em geral, atacando principalmente nos países em desenvolvimento. Com isso, criase a necessidade da descoberta de agentes terapêuticos para o tratamento da DP. A monoamina oxidase (MAO) é uma enzima de grande importância na neuroquímica, pois catalisa a desaminação oxidativa de aminas biogênicas, como monoaminas neurotransmissoras e neuromoduladoras, assim como monoaminas bioativas exógenas. Com base na especi...

  4. Modelagem Hidrológica Estocástica Aplicada ao Rio Tocantins para a Cidade de Marabá-PA

    Directory of Open Access Journals (Sweden)

    Renata Kelen Cardoso Câmara

    2016-03-01

    Full Text Available Resumo Estudos na área da hidrologia mostraram que podemos evitar desastres naturais através de previsões hidrológicas. Nesse trabalho foi utilizada a metodologia de Box-Jenkins de séries temporais multivariadas para previsão diária de nível fluviométrico do rio Tocantins para o município de Marabá-PA, que sofre anualmente com eventos de enchentes, ocasionado pelo aumento periódico do rio Tocantins e pela situação de vulnerabilidade da população que residem em áreas de riscos. Foram utilizados dados de níveis diários observados nas estações fluviométricas de Marabá e Carolina e Conceição do Araguaia da Agência Nacional de Águas (ANA, do período de 01/12/2008 a 31/03/2011. Evidenciou-se que o modelo ajustado conseguiu capturar a dinâmica das séries temporais, com bons prognósticos para o período de sete dias, com erro absoluto máximo de 0,08m, e com precisão na previsão acima de 99,00%. Assim, a pesquisa mostrou que o modelo de previsão teve um bom ajuste apresentando bons resultados, podendo ser utilizado como ferramentas de apoio para Defesa Civil, auxiliando no planejamento e preparo de ações preventivas para o município de Marabá.

  5. Métodos não-invasivos para análises hormonais aplicadas aos estudos de ecologia e etologia

    Directory of Open Access Journals (Sweden)

    Ricardo José Garcia Pereira

    2007-07-01

    Full Text Available O monitoramento endócrino pela mensuração de metabólitos urinários e fecais de hormônios esteróides tem se mostrado uma alternativa viável na investigação da fisiologia reprodutiva e do estresse em uma grande variedade de aves e mamíferos, domésticos e selvagens. Esta abordagem tem contribuído para uma maior integração da endocrinologia com estudos comportamentais e ecológicos, gerando informações mais detalhadas em diversas áreas tais como bem-estar animal, comportamento social, reprodução, biologia da conservação, biomedicina, entre outros. Todavia, o emprego desta metodologia no Brasil tem sido limitado principalmente à pesquisa de parâmetros reprodutivos e de estresse em espécies selvagens, não havendo até o momento trabalhos publicados utilizando a quantificação urinária ou fecal de glucocorticóides ou esteróides sexuais em animais domésticos. Desta forma, esta palestra tem como objetivo ilustrar alguns exemplos de estudos conduzidos no país envolvendo técnicas de monitoramento endócrino não-invasivo, assim como expor possíveis áreas de aplicação desta ferramenta em pesquisas com espécies domesticas.The endocrine monitoring through urinary and fecal steroid analysis has been proven a feasible alternative in the investigation of reproductive and stress physiology in a great variety of birds and mammals (domestic and wild species. This approach has contributed to enhance integration between endocrinology and behavioral/ ecological studies originating detailed information towards different areas, such as animal welfare, social behavior, reproduction, conservation biology, biomedicine, among others. In Brazil, however, its application has been limited mainly to the determination of reproductive and stress parameters in wild species, with no published articles employing measurement of urinary or fecal glucocorticoids or sexual steroids in domestic animals. Thus, the aim of this lecture is first show examples of Brazilian researches involving non-invasive endocrine monitoring, and secondly illustrate possible applications of this technology in studies involving domestic animals.

  6. Estudo da evaporação como tecnologia aplicada ao pré-tratamento de lixiviados de aterros sanitários

    OpenAIRE

    Moratelli, Letícia

    2013-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Ambiental, Florianópolis, 2013. A utilização de aterros sanitários para o tratamento e disposição de resíduos sólidos urbanos traz inúmeras vantagens, entretanto, apresenta como desvantagem a produção de lixiviado, líquido proveniente da degradação da matéria orgânica presente nos resíduos, bem como da água de infiltração que percola nas camadas de resíduos do aterro...

  7. Métodos não-invasivos para análises hormonais aplicadas aos estudos de ecologia e etologia

    OpenAIRE

    2007-01-01

    O monitoramento endócrino pela mensuração de metabólitos urinários e fecais de hormônios esteróides tem se mostrado uma alternativa viável na investigação da fisiologia reprodutiva e do estresse em uma grande variedade de aves e mamíferos, domésticos e selvagens. Esta abordagem tem contribuído para uma maior integração da endocrinologia com estudos comportamentais e ecológicos, gerando informações mais detalhadas em diversas áreas tais como bem-estar animal, comportamento social, reprodução, ...

  8. Abordagem adaptativa aplicada ao planejamento agregado da produção sob incertezas Adaptive approach applied to aggregate production planning under uncertainties

    Directory of Open Access Journals (Sweden)

    Oscar Salviano Silva Filho

    2010-03-01

    Full Text Available Um problema de planejamento agregado da produção, com incertezas sobre a flutuação da demanda, é formulado através de um modelo de otimização estocástica, com critério quadrático e restrições lineares. Dificuldades para encontrar uma solução ótima global para o problema levam à proposição de uma abordagem adaptativa de fácil implementação computacional que é baseada na formulação de um problema determinístico equivalente e que tem sua solução periodicamente revisada por meio de um procedimento clássico da literatura. Um exemplo, em que o sistema de balanço de estoque está sujeito à forte e fraca variabilidade da demanda real, é empregado para analisar o comportamento da abordagem proposta. Por fim, os resultados obtidos são comparados com outra abordagem subótima, cuja principal característica é não permitir revisões periódicas.A problem of aggregate production planning, with uncertainty about the fluctuation in demand, is formulated by a stochastic optimization model, with quadratic criterion and linear constraints. Difficulties in finding a global optimum solution to the problem lead to the proposal of an adaptive approach which is easy to implement computationally and which is based on the formulation of a deterministic equivalent problem, the solution for which is periodically reviewed through a classical procedure of literature. An example, where the inventory balance system is subject to weak and strong variability in actual demand, is employed to analyze the behavior of the proposed approach. Finally, the results provided by the proposed approach are compared with another suboptimal approach, the main characteristic of which is not allowing periodic reviews.

  9. Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis Biologia molecular aplicada ao diagnóstico laboratorial de endoftalmite bacteriana

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    Paulo José Martins Bispo

    2009-10-01

    Full Text Available Bacterial endophthalmitis is a serious but uncommon intraocular infection which frequently results in vision loss. Early diagnosis and appropriate therapy are associated with better visual outcome. Conventional microbiological methods are currently used for microbiological characterization of eyes with suspected endophthalmitis. However, the sensitivity of bacterial detection from aqueous and vitreous humor using microbiology techniques is poor, and time-consuming to confirm the results. The application of molecular methods enhances significantly laboratory confirmation of bacterial endophthalmitis, demanding a shorter time to draw a definitive result and thereby promoting the early initiation of a more specific therapy to limit the empirical or unnecessary use of broad-spectrum antibiotics. PCR-based techniques, including post-PCR methods such RFLP, DNA probe hybridization and DNA sequencing have been successfully used for the diagnostic elucidation of clinically suspected bacterial endophthalmitis cases, showing promising application in the routine practice of ocular microbiology laboratories.Endoftalmite bacteriana é uma infecção intraocular grave, mas de baixa frequência, podendo resultar em grande prejuízo visual. O diagnóstico precoce e a rápida instituição de terapia adequada estão associadas a um melhor prognóstico da doença. Os métodos microbiológicos convencionais são utilizados rotineiramente para caracterização microbiológica de olhos com suspeita de endoftalmite. No entanto, a sensibilidade de detecção bacteriana em amostras de humor aquoso e vítreo utilizando técnicas microbiológicas é baixa, além de demandar um maior tempo para a confirmação dos resultados. A utilização de métodos moleculares aumenta significativamente os casos de endoftalmite bacteriana confirmados laboratorialmente, com tempo menor para a liberação de um resultado definitivo, auxiliando assim a instituição precoce de uma terapia mais específica, limitando o uso empírico ou desnecessário de antibióticos de amplo espectro. A técnica de PCR e outras metodologias para análises pós-PCR como, RFLP, hibridização com sondas e sequenciamento do DNA, tem sido utilizadas com sucesso para elucidação diagnóstica em casos com suspeita clínica de endoftalmite bacteriana, demonstrando promissora aplicação para a rotina dos laboratórios de microbiologia ocular.

  10. The particle swarm optimization algorithm applied to nuclear systems surveillance test planning; Otimizacao aplicada ao planejamento de politicas de testes em sistemas nucleares por enxame de particulas

    Energy Technology Data Exchange (ETDEWEB)

    Siqueira, Newton Norat

    2006-12-15

    This work shows a new approach to solve availability maximization problems in electromechanical systems, under periodic preventive scheduled tests. This approach uses a new Optimization tool called PSO developed by Kennedy and Eberhart (2001), Particle Swarm Optimization, integrated with probabilistic safety analysis model. Two maintenance optimization problems are solved by the proposed technique, the first one is a hypothetical electromechanical configuration and the second one is a real case from a nuclear power plant (Emergency Diesel Generators). For both problem PSO is compared to a genetic algorithm (GA). In the experiments made, PSO was able to obtain results comparable or even slightly better than those obtained b GA. Therefore, the PSO algorithm is simpler and its convergence is faster, indicating that PSO is a good alternative for solving such kind of problems. (author)

  11. Densidade amostral aplicada ao monitoramento georreferenciado de lagartas desfolhadoras na cultura da soja Sample density applied to the georeferenced monitoring of defoliating caterpillars in soybean crop

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    Cinei Teresinha Riffel

    2012-12-01

    Full Text Available O conhecimento da distribuição espaço-temporal de insetos-praga na cultura da soja, por meio do uso de ferramentas da agricultura de precisão, tem sido apontado como uma importante estratégia no manejo integrado de pragas (MIP. Nesse sentido, o objetivo do trabalho foi avaliar a influência da densidade amostral no monitoramento de lagartas desfolhadoras na cultura da soja. O experimento foi conduzido em área experimental de 48,0ha, localizada no município de Júlio de Castilhos - RS, no ano agrícola 2008/2009. O monitoramento georreferenciado foi realizado seguindo três malhas amostrais regulares, 50x50m, 71x71m e 100x100m e também seguindo o método tradicional de amostragem. Durante todo o ciclo da cultura, e para cada malha amostral, foram realizadas cinco avaliações da infestação de lagartas, duas no estádio vegetativo e três no reprodutivo, com a utilização do pano-de-batida. Para analisar a distribuição espaço-temporal das lagartas na área, os dados foram submetidos à análise estatística descritiva e à geoestatística, utilizando semivariogramas e krigagem para elaboração dos mapas temáticos. Os resultados obtidos indicam que as grades amostrais avaliadas permitem caracterizar a distribuição espacial das lagartas e modelar a variabilidade espacial de lagartas na cultura da soja. A amostragem e monitoramento georreferenciado e posterior elaboração de mapas temáticos constituem-se numa alternativa potencial para agregarem-se às estratégias de MIP.The knowledge of the spatial and temporal distribution of pest insects on soybean crop through the use of precision agriculture tools, have been appointed as an important strategy in the integrated pest management (IPM. In this sense, the objective of this research was to evaluate the influence of sample density in the monitoring of defoliating caterpillars in soybean crop. The experiment was conducted in the experimental area of 47.98ha, located in Júlio de Castilhos - RS city, in 2008/2009 season. The georeferenced monitoring was carried out following three regular grids, 50x50m, 71x71m and 100x100m and also following the traditional method of sampling. During the entire crop cycle, and for each grid, there were conducted five evaluations of caterpillar infestations, two on the vegetative stage and three in the reproductive, using a beat cloth. To analyze the spatial and temporal distribution of caterpillars in the area, the data were submitted to descriptive statistical analysis and geostatistics analysis, using semivariograms and kriging to the elaboration of thematic maps. The results obtained indicated that the evaluated sample grids allowed to characterize the spatial distribution of caterpillars and modeled the spatial variability of caterpillars in soybean crop. The sampling and the georeferenced monitoring and further development of georeferenced thematic maps constitute a potential alternative to aggregate to the IPM strategies.

  12. UMA TECNOLOGIA DE PROCESSO APLICADA AO ACOMPANHANTE DO IDOSO HOSPITALIZADO PARA SUA INCLUSÃO PARTICIPATIVA NOS CUIDADOS DIÁRIOS

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    Maria Luiza de Oliveira Teixeira

    2009-01-01

    ser una tecnología de proceso que fue bien evaluada por los acompañantes, lo que los llevó a la autonomía compartida en el cuidado de los ancianos hospitalizados, según la Política Nacional de Humanización.

  13. Microscopia confocal reflectante aplicada ao diagnóstico do melanoma cutâneo Reflectance confocal microscopy in the diagnosis of cutaneous melanoma

    Directory of Open Access Journals (Sweden)

    Cintia Rito

    2009-12-01

    Full Text Available O melanoma cutâneo é um problema de saúde pública a nível mundial. Sua incidência tem aumentado, de forma marcante, nos últimos anos, e o diagnóstico e excisão precoces são essenciais para o bom prognóstico dos pacientes. Neste contexto, a dermatoscopia ganhou grande importância, nas últimas duas décadas, melhorando, de forma significativa, a acurácia do diagnóstico do melanoma, em estágios iniciais. Porém, existem algumas lesões benignas que apresentam dermatoscopia duvidosa, levando à realização de cirurgias desnecessárias. Mais recentemente, a microscopia confocal reflectante vem sendo introduzida como método diagnóstico auxiliar promissor, por ser um exame não-invasivo, realizado in vivo, de forma simples, indolor e de rápida execução. É a única técnica capaz de identificar estruturas celulares e examinar a epiderme e a derme papilar, com resolução semelhante à da histopatologia, com uma sensibilidade de 97,3%, e especificidade de 72,3% para o diagnóstico do melanoma cutâneo. É uma importante ferramenta diagnóstica, visto que não substitui o exame histopatológico realizado no pós-operatório, mas permite a abordagem racional das lesões com dermatoscopia duvidosa, evitando procedimentos cirúrgicos desnecessários.Skin melanoma is an international public health issue, with a considerable increase in frequency over the past few years. Early diagnosis and excision are essential for good patient prognosis. Over the past two decades dermoscopy has gained significance due to a major improvement in the accuracy of skin melanoma diagnosis in its early stage. However, there are some benign lesions of questionable dermoscopy, which may lead to the performance of unnecessary surgery. Recently, reflectance confocal microscopy has been introduced as a promising supplementary diagnostic method. It is a noninvasive, in vivo, simple, painless and quick exam. It is the only technique capable of identifying cellular structures and to examine the epidermis and papillary dermis with a resolution similar to that of histopathology, with a sensitivity of 97.3% and specificity of 72.3 % in the diagnosis of cutaneous melanoma. This is an important diagnostic tool, because it does not substitute post-surgical histopathological examination and allows for the rational assessment of lesions of questionable dermoscopy, thus avoiding unnecessary surgical procedures.

  14. Espectrometria de massas com ionização por "electrospray" aplicada ao estudo de espécies inorgânicas e organometálicas

    Directory of Open Access Journals (Sweden)

    Moraes Maria Carolina B.

    2003-01-01

    Full Text Available Although electrospray ionization mass spectrometry (ES-MS has been extensively applied to study organic and biochemical species, it is also specially suitable to study inorganic and organo-metallic species. Such species, even those that are low-volatile or thermo-unstable, can be easily ionized or simply transferred from liquid to gas phase by electrospray. However, chemical transformations frequently occur during the process. This paper discusses the fundamental aspects of electrospray ionization as well as its analytical applicability to inorganic and organo-metallic species in order to spread the technique and make its characteristics more familiar to potential users.

  15. Práticas contemporaneas de geocomputação aplicadas ao ambiente urbano digital Contemporary pratices in geocomputing applied to digital urban environment

    Directory of Open Access Journals (Sweden)

    Otávio Martins Peres

    2011-12-01

    Full Text Available A dinâmica espaçotemporal urbana é um processo de difícil apreensão, pela elevada quanti-dade de fatores presentes na cidade e na paisagem que integra, pelas suas estreitas relações e diferentes escalas. Para esse enfrentamento, a ciência urbana demanda novas ferramentas que contemporaneamente ganham em rigor geoespacial, possibilidades representativas, e simulta-neamente passam a envolver a variável temporal. Associando conceitos e práticas da ciência do espaço, da modelagem urbana e dos sistemas computacionais, o objetivo do trabalho é trazer à tona um conjunto de instrumentos para abordar o ambiente urbano de modo digital, possibilitados a partir de recursos das geotecnologias. O trabalho apresenta aplicações de dife-rentes sistemas de informações geográficas (SIG no campo da ciência, dos serviços e para auxiliar no suporte à tomada de decisões. Da convergência entre as geotecnologias, das cida-des visuais e da geocomputação, ambientes urbanos do presente, passado e futuro podem ser virtualmente replicados em ambientes digitais, permitindo avanços nos campos analítico e experimental, e podem ser auxiliares na produção de conhecimento a respeito do fenômeno urbano.

  16. Uma ferramenta para análise de redes de interações polinizador-planta aplicada ao portal IABIN-PTN.

    OpenAIRE

    Paulo Venancio Lopes

    2012-01-01

    O planeta está perdendo rapidamente sua biodiversidade, e isto se dá em boa medida pelo crescimento da população humana. Com ela cresceu a demanda por alimentos e matérias primas, e por conseqüência, aumentou a quantidade de terras usadas para sua produção. Esta demanda por terras aráveis e a busca por minérios fez com que a natureza entrasse em crise e a perda dos polinizadores, principalmente as abelhas, sem dúvida merece destaque. Isto por que estas espécies ...

  17. Proteômica e transcriptômica aplicadas ao estudo da variabilidade do veneno de Bothrops jararaca (serpentes:viperidae)

    OpenAIRE

    André Zelanis Palitot Pereira

    2011-01-01

    Estudos prévios demonstraram que as atividades biológicas do veneno da serpente Bothrops jararaca sofrem significantes modificações ontogenéticas. Neste estudo é apresentada uma análise comparativa do proteoma, peptidoma e transcriptoma da glândula de veneno de filhotes e adultos de B. jararaca, correlacionando os resultados obtidos com algumas características funcionais dos venenos. Venenos de 694 filhotes de até duas semanas de idade e 110 adultos, provenientes do Estado de São Paulo foram ...

  18. Advantages of electrical propulsion applied to the urban transportation with express lines; Vantagens da propulsao eletrica aplicada ao transporte urbano em linhas expressas

    Energy Technology Data Exchange (ETDEWEB)

    Schwob, Marcelo Rousseau Valenca; Rodrigues, Joaquim Augusto Pinto; Henriques Junior, Mauricio F.; Dresch, Patricia Miranda; Dantas, Fabricio dos Santos [Instituto Nacional de Tecnologia (INT), Rio de Janeiro, RJ (Brazil). Div. de Energia], E-mail: energia@int.gov.br; Bernardes, Fernanda Manhaes [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil)

    2010-07-01

    This paper intends to compare the various possible solutions for urban bus propulsion under the environmental aspects (gaseous emissions, vibrations and noises), energy efficiency and energetic operational costs, facing the evident signals of energy inefficiency and environmental limitations presented at urban highway transports of large cities.

  19. Estrategias flexibilizadoras aplicadas por las empresas del sector avícola del estado Zulia

    Directory of Open Access Journals (Sweden)

    María Bonomie

    2008-01-01

    Full Text Available El artículo tiene como objetivo, analizar las estrategias flexibilizadoras aplicadas en el proceso productivo por las empresas del sector avícola, específicamente las empresas productoras de pollo del estado Zulia. Para tal fin, se describe el proceso productivo de estas empresas y además se identifican las estrategias aplicadas considerando los acontecimientos económicos influenciados por cambios políticos que en los últimos años han incidido en las empresas productoras de pollo. Se realizó una revisión bibliográfica para determinar los criterios de clasificación de las estrategias, asumiendo la establecida por Gamboa y col. (2001. Se diseñó un instrumento semiestructurado, el cual fue administrado a los gerentes y trabajadores de las empresas analizadas. Los resultados obtenidos reflejan, que las empresas utilizan las estrategias con la finalidad de reestructurar y modernizar sus procesos productivos, mejorar continuamente sus productos e introducir nueva tecnología. Se concluye que estas empresas logran incrementar la productividad, aumentando la cantidad de productos ante la utilización óptima de los recursos humanos y tecnológicos, optimización que implica una reducción de costos de producción para poder expandirse y ofrecer precios competitivos en el mercado.

  20. SRAO: the first southern robotic AO system

    Science.gov (United States)

    Law, Nicholas M.; Ziegler, Carl; Tokovinin, Andrei

    2016-08-01

    We present plans for SRAO, the first Southern Robotic AO system. SRAO will use AO-assisted speckle imaging and Robo-AO-heritage high efficiency observing to confirm and characterize thousands of planet candidates produced by major new transit surveys like TESS, and is the first AO system to be capable of building a comprehensive several-thousand-target multiplicity survey at sub-AU scales across the main sequence. We will also describe results from Robo-AO, the first robotic LGS-AO system. Robo-AO has observed tens of thousands of Northern targets, often using a similar speckle or Lucky-Imaging assisted mode. SRAO will be a moderate-order natural-guide-star adaptive optics system which uses an innovative photoncounting wavefront sensor and EMCCD speckle-imaging camera to guide on faint stars with the 4.1m SOAR telescope. The system will produce diffraction-limited imaging in the NIR on targets as faint as mν = 16. In AO-assisted speckle imaging mode the system will attain the 30-mas visible diffraction limit on targets at least as faint as mν = 17. The system will be the first Southern hemisphere robotic adaptive optics system, with overheads an order of magnitude smaller than comparable systems. Using Robo-AO's proven robotic AO software, SRAO will be capable of observing overheads on sub-minute scales, allowing the observation of at least 200 targets per night. SRAO will attain three times the angular resolution of the Palomar Robo-AO system in the visible.

  1. Psicologia del color aplicada a los cursos virtuales para mejorar el nivel de aprendizaje en los estudiantes

    OpenAIRE

    Canté García, Julio Francisco

    2017-01-01

    La psicología del color aplicada a la elaboración de la interfaz que se diseñe para un curso virtual jugará un papel importante en las posibilidades de aprender. Si no es elaborada de manera adecuada, repercute inmediatamente en la motivación y el aprendizaje del estudiante. La interfaz gráfica ha de ser estimulante, explícita, consistente y funcional, para que el alumno la comprenda y explore fácilmente. La psicologia del color aplicada a l'elaboració de la interfície que es dissenyi per ...

  2. II SEMINARIO INTERNACIONAL EN CIENCIAS APLICADAS “Una mirada hacia la innovación y el desarrollo sostenible”

    OpenAIRE

    Diana Verónica Cortés-Espinosa; et. al.

    2013-01-01

    INTRODUCCIÓN La Química, Biología y las Matemáticas son el pilar de las ciencias aplicadas. La investigación en el área básica se ve reflejada en el desarrollo de disciplinas como las ciencias de la salud, ingenierías y ciencias ambientales. De esta forma, la integración entre la investigación básica y las ciencias aplicadas es fundamental en el desarrollo del profesional moderno. Considerando lo anterior la Facultad de Ciencias Exactas Físicas y Naturales organiza el segundo seminario in...

  3. Simultaneous Low- and High-Grade Primary Leiomyosarcomas in Two Separate Organs in a Thirty-Year Survivor of Hereditary Retinoblastoma

    Science.gov (United States)

    Moore, Katherine; Tawfik, Ossama; Hays, Allyson; Opole, Isaac

    2015-01-01

    Patients with hereditary retinoblastoma have a heightened risk of developing subsequent bone and soft tissue sarcomas, exacerbated by radiation and alkylating chemotherapy. Secondary leiomyosarcomas are rare. A 29-year-old patient with bilateral retinoblastoma history presented with concurrent, independent malignancies found to be Rb-suppressed uterine and Rb-positive bladder leiomyosarcomas. She had undergone both ifosfamide chemotherapy and radiation therapy but demonstrated rare simultaneous primary leiomyosarcomas not previously described in association with ifosfamide. This rare case underlines the protean genetic predisposition to malignancy in this patient population, heightening awareness of lifelong malignancy potential, and the necessity for continuous periodic screening. PMID:26843997

  4. MagAO: status and science

    Science.gov (United States)

    Morzinski, Katie M.; Close, Laird M.; Males, Jared R.; Hinz, Phil M.; Esposito, Simone; Riccardi, Armando; Briguglio, Runa; Follette, Katherine B.; Pinna, Enrico; Puglisi, Alfio; Vezilj, Jennifer; Xompero, Marco; Wu, Ya-Lin

    2016-07-01

    "MagAO" is the adaptive optics instrument at the Magellan Clay telescope at Las Campanas Observatory, Chile. MagAO has a 585-actuator adaptive secondary mirror and 1000-Hz pyramid wavefront sensor, operating on natural guide stars from R-magnitudes of -1 to 15. MagAO has been in on-sky operation for 166 nights since installation in 2012. MagAO's unique capabilities are simultaneous imaging in the visible and infrared with VisAO and Clio, excellent performance at an excellent site, and a lean operations model. Science results from MagAO include the first ground-based CCD image of an exoplanet, demonstration of the first accreting protoplanets, discovery of a new wide-orbit exoplanet, and the first empirical bolometric luminosity of an exoplanet. We describe the status, report the AO performance, and summarize the science results. New developments reported here include color corrections on red guide stars for the wavefront sensor; a new field stop stage to facilitate VisAO imaging of extended sources; and eyepiece observing at the visible-light diffraction limit of a 6.5-m telescope. We also discuss a recent hose failure that led to a glycol coolant leak, and the recovery of the adaptive secondary mirror (ASM) after this recent (Feb. 2016) incident.

  5. MagAO: Status and Science

    CERN Document Server

    Morzinski, Katie M; Males, Jared R; Hinz, Phil M; Esposito, Simone; Riccardi, Armando; Briguglio, Runa; Follette, Katherine B; Pinna, Enrico; Puglisi, Alfio; Vezilj, Jennifer; Xompero, Marco; Wu, Ya-Lin

    2016-01-01

    "MagAO" is the adaptive optics instrument at the Magellan Clay telescope at Las Campanas Observatory, Chile. MagAO has a 585-actuator adaptive secondary mirror and 1000-Hz pyramid wavefront sensor, operating on natural guide stars from $R$-magnitudes of -1 to 15. MagAO has been in on-sky operation for 166 nights since installation in 2012. MagAO's unique capabilities are simultaneous imaging in the visible and infrared with VisAO and Clio, excellent performance at an excellent site, and a lean operations model. Science results from MagAO include the first ground-based CCD image of an exoplanet, demonstration of the first accreting protoplanets, discovery of a new wide-orbit exoplanet, and the first empirical bolometric luminosity of an exoplanet. We describe the status, report the AO performance, and summarize the science results. New developments reported here include color corrections on red guide stars for the wavefront sensor, a new field stop stage to facilitate VisAO imaging of extended sources; and eye...

  6. Access to the ophthalmic artery by retrograde approach through the posterior communicating artery for intra-arterial chemotherapy of retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Pham, Chi-Tuan; Blanc, Raphael; Pistocchi, Silvia; Bartolini, Bruno; Piotin, Michel [Fondation Rothschild Hospital, Department of Interventional Neuroradiology, Paris (France); Lumbroso-Le Rouic, Livia [Institut Curie, Department of Ocular Oncology, Paris (France)

    2012-08-15

    Intra-arterial infusion of chemotherapy into the ophthalmic artery for treatment of retinoblastoma has been realized after catheterization of the internal carotid and temporary balloon occlusion beyond the orifice of the ophthalmic artery, or more recently after superselective canulation of the ophthalmic artery by a microcatheter. The superselective catheterization of the ophthalmic artery could be cumbersome because of the implantation of the ostium on the carotid siphon or because of the tortuosity of the carotid siphon. We report our experience of using a retrograde approach through the posterior communicating artery that allows a more direct angle of access to the origin of the ophthalmic artery. (orig.)

  7. Transcriptional regulatory program in wild-type and retinoblastoma gene-deficient mouse embryonic fibroblasts during adipocyte differentiation

    DEFF Research Database (Denmark)

    Hakim-Weber, Robab; Krogsdam, Anne-M; Jørgensen, Claus

    2011-01-01

    Although many molecular regulators of adipogenesis have been identified a comprehensive catalogue of components is still missing. Recent studies showed that the retinoblastoma protein (pRb) was expressed in the cell cycle and late cellular differentiation phase during adipogenesis. To investigate...... of 64 deregulated genes showed that the Rb-/- MEF model exhibits a brown(-like) adipocyte phenotype. Additionally, the analysis results indicate a different or additional role for pRb family member involvement in the lineage commitment. CONCLUSION: In this study a number of commonly modulated genes...

  8. Dissecting functions of the retinoblastoma tumor suppressor and the related pocket proteins by integrating genetic, cell biology, and electrophoretic techniques

    DEFF Research Database (Denmark)

    Hansen, Klaus; Lukas, J; Holm, K;

    1999-01-01

    The members of the 'pocket protein' family, comprising the retinoblastoma tumor suppressor (pRB) and its relatives, p107 and p130, negatively regulate cell proliferation and modulate fundamental biological processes including embryonic development, differentiation, homeostatic tissue renewal...... phosphorylation events on multiple serine and threonine residues of pRB, p107, and p130, events which are carried out, at least in part, by the cyclin-dependent kinases that form the key elements of the cell cycle machinery. Here we discuss the recently obtained new insights into the diverse functions of the pRB...

  9. Sensibilidade de cultivares de algodoeiro ao cloreto de mepiquat Sensibility of cotton cultivars to mepiquat chloride

    Directory of Open Access Journals (Sweden)

    Julio Cesar Bogiani

    2009-10-01

    Full Text Available O objetivo deste trabalho foi avaliar a sensibilidade de cultivares de algodoeiro ao cloreto de mepiquat e verificar se ela está relacionada ao ciclo ou à arquitetura da planta. Foi realizado um experimento em casa de vegetação com seis cultivares de algodoeiro - FiberMax 966, FiberMax 977, Delta Penta, Delta Opal, FMT 501 e FMT 701 - e quatro doses do regulador vegetal à base de cloreto de mepiquat - 0, 7,5, 15 e 22,5 g ha-1 de i.a. -, em delineamento de blocos ao acaso, com arranjo fatorial 6x4 e quatro repetições. As parcelas experimentais foram constituídas por vasos de 12 L com duas plantas em cada um. As doses do regulador vegetal foram aplicadas sem adjuvante, no estádio de aparecimento dos botões florais, aos 34 dias após a emergência das plântulas. Paralelamente ao experimento com doses, a retenção do regulador pelas folhas das cultivares foi quantificada. O crescimento em altura das plantas de algodoeiro é diminuído com a aplicação do regulador vegetal, e esse efeito se intensifica com o aumento da dosagem aplicada. Existe diferença entre as cultivares estudadas quanto à sensibilidade ao cloreto de mepiquat, sendo que as mais precoces são mais sensíveis. Além da sensibilidade diferenciada, a arquitetura das plantas pode possibilitar maior ou menor deposição do regulador sobre as folhas.The objective of this work was to assess the sensibility of cotton cultivars to mepiquat chloride and to verify if it is related to plant cycle or architecture. A green-house experiment was carried out with six cotton cultivars - FiberMax 966, FiberMax 977, Delta Penta, Delta Opal, FMT 501 e FMT 701 -, and four concentrations of mepiquat chloride plant growth regulator - 0, 7,5, 15 and 22,5 g ha-1 of the active ingredient - in complete block design with a 6x4 factorial arrangement and four replicates. The plots consisted of 12 L pots with two plants per pot. Regulator doses were applied without adjuvants at the beginning of

  10. Expression levels of autophagy related proteins and their prognostic significance in retinocytoma and retinoblastoma

    Institute of Scientific and Technical Information of China (English)

    Yue; Liu; Shang-Tao; Wan; Ping; Zhang; Wen-Xin; Zhang; Jian-Ling; Zheng; Jian-Xian; Lin; Yong-Ping; Li

    2014-01-01

    ·AIM: To discuss the prognostic significant of autophagy related proteins(ARPs) in retinoblastoma(RB)and to find the molecular marker to distinguish retinocytoma(RC) and RB by investigating the different expression profiling of microtubule-associated protein light chain 3(LC3B) and other ARPs in RC and RB.·METHODS: Specimens with retinocytoma region(RCR)or mainly composed with Flexner-Winterstein rosettes(FWR) were screen out from 219 paraffin-embedded RB samples and respectively taken as RCR group and FWR group. Others were taken as undifferentiated(UD) group.Immunochemistry(IHC) of LC3 B and electronic microscopy was used to identify autophagy. The IHC scores of LC3 B and other ARPs, such as Beclin, PTEN,p27, p16INK4 a, mTOR and BCL-2 were compared and correlation analysis was applied to find potential proteins which may involve in autophagy regulation. The prognostics significance of LC3 B was evaluated by comparing the high risk features(HRFs) in 3 groups of total 219 samples.·RESULTS: Twenty-one specimens with RCR and 36 specimens mainly composed with FWR were screen out.RCR cell had a high level of LC3 B and lots of autophagic vacuoles. Beclin, PTEN, p27 had positive correlation with LC3, and p16INK4 ahad negative correlation, while the expression of mTOR and BCL-2 in RCR and RB region did not show any difference. Cases with RCR had lower rate of HRFs than undifferentiated cases.·CONCLUSION: ARPs had different expression pattern between RCR and other pathological types of RB, and could be ideal markers to distinguish RC from RB. Our finding indicated cases with RCR had favorable prognosis just like those with FWR.

  11. Retinoblastoma-binding protein 1 has an interdigitated double Tudor domain with DNA binding activity.

    Science.gov (United States)

    Gong, Weibin; Wang, Jinfeng; Perrett, Sarah; Feng, Yingang

    2014-02-21

    Retinoblastoma-binding protein 1 (RBBP1) is a tumor and leukemia suppressor that binds both methylated histone tails and DNA. Our previous studies indicated that RBBP1 possesses a Tudor domain, which cannot bind histone marks. In order to clarify the function of the Tudor domain, the solution structure of the RBBP1 Tudor domain was determined by NMR and is presented here. Although the proteins are unrelated, the RBBP1 Tudor domain forms an interdigitated double Tudor structure similar to the Tudor domain of JMJD2A, which is an epigenetic mark reader. This indicates the functional diversity of Tudor domains. The RBBP1 Tudor domain structure has a significant area of positively charged surface, which reveals a capability of the RBBP1 Tudor domain to bind nucleic acids. NMR titration and isothermal titration calorimetry experiments indicate that the RBBP1 Tudor domain binds both double- and single-stranded DNA with an affinity of 10-100 μM; no apparent DNA sequence specificity was detected. The DNA binding mode and key interaction residues were analyzed in detail based on a model structure of the Tudor domain-dsDNA complex, built by HADDOCK docking using the NMR data. Electrostatic interactions mediate the binding of the Tudor domain with DNA, which is consistent with NMR experiments performed at high salt concentration. The DNA-binding residues are conserved in Tudor domains of the RBBP1 protein family, resulting in conservation of the DNA-binding function in the RBBP1 Tudor domains. Our results provide further insights into the structure and function of RBBP1.

  12. Hepatitis C virus induces E6AP-dependent degradation of the retinoblastoma protein.

    Directory of Open Access Journals (Sweden)

    Tsubasa Munakata

    2007-09-01

    Full Text Available Hepatitis C virus (HCV is a positive-strand RNA virus that frequently causes persistent infections and is uniquely associated with the development of hepatocellular carcinoma. While the mechanism(s by which the virus promotes cancer are poorly defined, previous studies indicate that the HCV RNA-dependent RNA polymerase, nonstructural protein 5B (NS5B, forms a complex with the retinoblastoma tumor suppressor protein (pRb, targeting it for degradation, activating E2F-responsive promoters, and stimulating cellular proliferation. Here, we describe the mechanism underlying pRb regulation by HCV and its relevance to HCV infection. We show that the abundance of pRb is strongly downregulated, and its normal nuclear localization altered to include a major cytoplasmic component, following infection of cultured hepatoma cells with either genotype 1a or 2a HCV. We further demonstrate that this is due to NS5B-dependent ubiquitination of pRb and its subsequent degradation via the proteasome. The NS5B-dependent ubiquitination of pRb requires the ubiquitin ligase activity of E6-associated protein (E6AP, as pRb abundance was restored by siRNA knockdown of E6AP or overexpression of a dominant-negative E6AP mutant in cells containing HCV RNA replicons. E6AP also forms a complex with pRb in an NS5B-dependent manner. These findings suggest a novel mechanism for the regulation of pRb in which the HCV NS5B protein traps pRb in the cytoplasm, and subsequently recruits E6AP to this complex in a process that leads to the ubiquitination of pRb. The disruption of pRb/E2F regulatory pathways in cells infected with HCV is likely to promote hepatocellular proliferation and chromosomal instability, factors important for the development of liver cancer.

  13. Use of intra-arterial chemotherapy for retinoblastoma:results of a survey

    Institute of Scientific and Technical Information of China (English)

    Nathalia; Grigorovski; Evandro; Lucena; Clarissa; Mattosinho; Andreu; Parareda; Sima; Ferman; Jaume; Catalá; Guillermo; Chantada

    2014-01-01

    ·AIM: To obtain baseline knowledge about the current use of intra-arterial chemotherapy(SSOAIC) in centers worldwide.·METHODS: A survey including questions about the use of SSOAIC was emailed to retinoblastoma experts.·RESULTS:Seventy-nine(response rate 69.9%) doctors from 63 centers in 35 countries responded. Thirty-one centers from 19 countries use SSOAIC. Twelve performed more than 50 procedures. Melphalan is the most commonly used drug but 15 centers use more than one drug. First line therapy for advanced unilateral disease is the most common use of SSOAIC(74.2%). Centers with larger experience(>50 applications) were less likely using melphalan alone(P =0.06) and significantly more likely using SSOAIC in more situations such as second line in preference to radiotherapy P =0.05. Nineteen(61.2%)stated that SSOAIC improved their results and 21(77.8%)reported less toxicity compared to other treatments.Three centers reported that SSOAIC did not improve their results. There were regional variations in the use of SSOAIC which is used more frequently as secondary treatment in Europe compared to the USA and Japan.Ten centers identified cost is the major limiting factor for SSOAIC.· CONCLUSION: SSOAIC is used in an increasing number of centers worldwide with regional variations.Centers with more experience in SSOAIC use it in more situations including other drugs than melphalan. The majority of the centers using this technique reportedimproved results and few complications.

  14. Conditional Deletion of the Retinoblastoma (Rb) Gene in Ovarian Granulosa Cells Leads to Premature Ovarian Failure

    Science.gov (United States)

    Andreu-Vieyra, Claudia; Chen, Ruihong; Matzuk, Martin M.

    2008-01-01

    The retinoblastoma protein (RB) regulates cell proliferation and survival by binding to the E2F family of transcription factors. Recent studies suggest that RB also regulates differentiation in a variety of cell types, including myocytes, neurons, adipocytes, and chondrocytes. Rb mutations have been found in ovarian cancer; however, the role of RB in normal and abnormal ovarian function remains unclear. To test the hypothesis that loss of Rb induces ovarian tumorigenesis, we generated an ovarian granulosa cell conditional knockout of Rb (Rb cKO) using the Cre/lox recombination system. Rb cKO females showed 100% survival and no ovarian tumor formation through 9 months of age, but they developed progressive infertility. Prepubertal Rb cKO females showed increased ovulation rates compared with controls, correlating with increased follicle recruitment, higher Fshr and Kitl mRNA levels, and lower anti-Müllerian hormone levels. In contrast, the ovulation rate of 6-wk-old females was similar to that of controls. Morphometric analysis of Rb cKO ovaries from 6-wk-old and older females showed increased follicular atresia and apoptosis. Rb cKO ovaries and preantral follicles had abnormal levels of known direct and indirect target genes of RB, including Rbl2/p130, E2f1, Ccne2, Myc, Fos, and Tgfb2. In addition, preantral follicles showed increased expression of the granulosa cell differentiation marker Inha, decreased levels of Foxl2 and Cyp19a1 aromatase, and abnormal expression of the nuclear receptors Nr5a1, Nr5a2, and Nr0b1. Taken together, our results suggest that RB is required for the temporal-specific pattern of expression of key genes involved in follicular development. PMID:18599617

  15. NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein.

    Science.gov (United States)

    Tooley, Christine E Schaner; Petkowski, Janusz J; Muratore-Schroeder, Tara L; Balsbaugh, Jeremy L; Shabanowitz, Jeffrey; Sabat, Michal; Minor, Wladek; Hunt, Donald F; Macara, Ian G

    2010-08-26

    The post-translational methylation of alpha-amino groups was first discovered over 30 years ago on the bacterial ribosomal proteins L16 and L33 (refs 1, 2), but almost nothing is known about the function or enzymology of this modification. Several other bacterial and eukaryotic proteins have since been shown to be alpha-N-methylated. However, the Ran guanine nucleotide-exchange factor, RCC1, is the only protein for which any biological function of alpha-N-methylation has been identified. Methylation-defective mutants of RCC1 have reduced affinity for DNA and cause mitotic defects, but further characterization of this modification has been hindered by ignorance of the responsible methyltransferase. All fungal and animal N-terminally methylated proteins contain a unique N-terminal motif, Met-(Ala/Pro/Ser)-Pro-Lys, indicating that they may be targets of the same, unknown enzyme. The initiating Met is cleaved, and the exposed alpha-amino group is mono-, di- or trimethylated. Here we report the discovery of the first alpha-N-methyltransferase, which we named N-terminal RCC1 methyltransferase (NRMT). Substrate docking and mutational analysis of RCC1 defined the NRMT recognition sequence and enabled the identification of numerous new methylation targets, including SET (also known as TAF-I or PHAPII) and the retinoblastoma protein, RB. Knockdown of NRMT recapitulates the multi-spindle phenotype seen with methylation-defective RCC1 mutants, demonstrating the importance of alpha-N-methylation for normal bipolar spindle formation and chromosome segregation.

  16. PCR detection of retinoblastoma gene deletions in radiation-induced mouse lung adenocarcinomas

    Energy Technology Data Exchange (ETDEWEB)

    Churchill, M.E.; Gemmell, M.A.; Woloschak, G.E.

    1993-04-01

    From 1971 to 1986, Argonne National Laboratory conducted a series of large-scale studies of tumor incidence in 40,000 BCF{sub 1} mice irradiated with {sup 60}Co {gamma} rays or JANUS fission-spectrum neutrons; normal and tumor tissues from mice in these studies were preserved in paraffin blocks. A polymerase chain reaction (PCR) technique has been developed to detect deletions in the mouse retinoblastoma (mRb) gene in the paraffin-embedded tissues. Microtomed sections were used as the DNA source in PCR reaction mixtures. Six mRb gene exon fragments were amplified in a 40-cycle, 3-temperature PCR protocol. The absence of any of these fragments (relative to control PCR products) on a Southern blot indicated a deletion of that portion of the mRb gene. The tumors chosen for analysis were lung adenocarcinomas that were judged to be the cause of death in post-mortem analyses. Spontaneous tumors as well as those from irradiated mice (569 cGy of {sup 60}Co {gamma} rays or 60 cGy of JANUS neutrons, doses that have been found to have approximately equal biological effectiveness in the BCF, mouse) were analyzed for mRb deletions. In all normal mouse tissues studies, all six mRb exon fragments were present on Southem blots. Tumors in six neutron-irradiated mice also had no mRb deletions. However, I of 6 tumors from {gamma}-irradiated mice and 6 of 18 spontaneous tumors from unirradiated mice had a deletion in one or both mRb alleles. All deletions detected were in the 5{prime} region of the mRb gene.

  17. PCR detection of retinoblastoma gene deletions in radiation-induced mouse lung adenocarcinomas

    Energy Technology Data Exchange (ETDEWEB)

    Churchill, M.E.; Gemmell, M.A.; Woloschak, G.E.

    1993-01-01

    From 1971 to 1986, Argonne National Laboratory conducted a series of large-scale studies of tumor incidence in 40,000 BCF[sub 1] mice irradiated with [sup 60]Co [gamma] rays or JANUS fission-spectrum neutrons; normal and tumor tissues from mice in these studies were preserved in paraffin blocks. A polymerase chain reaction (PCR) technique has been developed to detect deletions in the mouse retinoblastoma (mRb) gene in the paraffin-embedded tissues. Microtomed sections were used as the DNA source in PCR reaction mixtures. Six mRb gene exon fragments were amplified in a 40-cycle, 3-temperature PCR protocol. The absence of any of these fragments (relative to control PCR products) on a Southern blot indicated a deletion of that portion of the mRb gene. The tumors chosen for analysis were lung adenocarcinomas that were judged to be the cause of death in post-mortem analyses. Spontaneous tumors as well as those from irradiated mice (569 cGy of [sup 60]Co [gamma] rays or 60 cGy of JANUS neutrons, doses that have been found to have approximately equal biological effectiveness in the BCF, mouse) were analyzed for mRb deletions. In all normal mouse tissues studies, all six mRb exon fragments were present on Southem blots. Tumors in six neutron-irradiated mice also had no mRb deletions. However, I of 6 tumors from [gamma]-irradiated mice and 6 of 18 spontaneous tumors from unirradiated mice had a deletion in one or both mRb alleles. All deletions detected were in the 5[prime] region of the mRb gene.

  18. Disruption of retinoblastoma protein expression in the intestinal epithelium impairs lipid absorption.

    Science.gov (United States)

    Choi, Pamela M; Guo, Jun; Erwin, Christopher R; Wandu, Wambui S; Leinicke, Jennifer A; Xie, Yan; Davidson, Nicholas O; Warner, Brad W

    2014-05-15

    We previously demonstrated increased villus height following genetic deletion, or knockout, of retinoblastoma protein (Rb) in the intestinal epithelium (Rb-IKO). Here we determined the functional consequences of augmented mucosal growth on intestinal fat absorption and following a 50% small bowel resection (SBR). Mice with constitutively disrupted Rb expression in the intestinal epithelium (Rb-IKO) along with their floxed (wild-type, WT) littermates were placed on a high-fat diet (HFD, 42% kcal fat) for 54 wk. Mice were weighed weekly, and fat absorption, indirect calorimetry, and MRI body composition were measured. Rb-IKO mice were also subjected to a 50% SBR, followed by HFD feeding for 33 wk. In separate experiments, we examined intestinal fat absorption in mice with conditional (tamoxifen-inducible) intestinal Rb (inducible Rb-IKO) deletion. Microarray revealed that the transcriptional expression of lipid absorption/transport genes was significantly reduced in constitutive Rb-IKO mice. These mice demonstrated greater mucosal surface area yet manifested paradoxically impaired intestinal long-chain triglyceride absorption and decreased cholesterol absorption. Despite attenuated lipid absorption, there were no differences in metabolic rate, body composition, and weight gain in Rb-IKO and WT mice at baseline and following SBR. We also confirmed fat malabsorption in inducible Rb-IKO mice. We concluded that, despite an expanded mucosal surface area, Rb-IKO mice demonstrate impaired lipid absorption without compensatory alterations in energy homeostasis or body composition. These findings underscore the importance of delineating structural/functional relationships in the gut and suggest a previously unknown role for Rb in the regulation of intestinal lipid absorption.

  19. Distinct and site-specific phosphorylation of the retinoblastoma protein at serine 612 in differentiated cells.

    Directory of Open Access Journals (Sweden)

    Takayuki Hattori

    Full Text Available The retinoblastoma susceptibility protein (pRB is a phosphoprotein that regulates cell cycle progression at the G1/S transition. In quiescent and early G1 cells, pRB predominantly exists in the active hypophosphorylated form. The cyclin/cyclin-dependent protein kinase complexes phosphorylate pRB at the late G1 phase to inactivate pRB. This event leads to the dissociation and activation of E2F family transcriptional factors. At least 12 serine/threonine residues in pRB are phosphorylated in vivo. Although there have been many reports describing bulk phosphorylation of pRB, detail research describing the function of each phosphorylation site remains unknown. Besides its G1/S inhibitory function, pRB is involved in differentiation, prevention of cell death and control of tissue fate. To uncover the function of phosphorylation of pRB in various cellular conditions, we have been investigating phosphorylation of each serine/threonine residue in pRB with site-specific phospho-serine/threonine antibodies. Here we demonstrate that pRB is specifically phosphorylated at Ser612 in differentiated cells in a known kinase-independent manner. We also found that pRB phosphorylated at Ser612 still associates with E2F-1 and tightly binds to nuclear structures including chromatin. Moreover, expression of the Ser612Ala mutant pRB failed to induce differentiation. The findings suggest that phosphorylation of Ser612 provides a distinct function that differs from the function of phosphorylation of other serine/threonine residues in pRB.

  20. Effects of salvianolic acid B on in vitro growth inhibition and apoptosis induction of retinoblastoma cells

    Science.gov (United States)

    Liu, Xing-An

    2012-01-01

    AIM To observe the effects of salvianolic acid B (SalB) on in vitro growth inhibition and apoptosis induction of retinoblastoma HXO-RB44 cells. METHODS The effects of SalB on the HXO-RB44 cells proliferation in vitro were observed by MTT colorimetric method. The morphological changes of apoptosis before and after the treatment of SalB were observed by Hoechst 33258 fluorescent staining method. Apoptosis rate and cell cycle changes of HXO-RB44 cells were detected by flow cytometer at 48 hours after treated by SalB. The expression changes of Caspase-3 protein in HXO-RB44 cells were detected by Western Blot. RESULTS SalB significantly inhibited the growth of HXO-RB44 cells, while the inhibition was in a concentration-and time-dependent manner. The results of fluorescent staining method indicated that HXO-RB44 cells showed significant phenomenon of apoptosis including karyorrhexis, fragmentation and the formation of apoptotic bodies, etc. after 24, 48 and 72 hours co-culturing of SalB and HXO-RB44 cells. The results of flow cytometer showed that the apoptosis rate and the proportion of cells in S phase were gradually increased at 48 hours and 72 hours after treated by different concentrations of SalB. Western Blot strip showed that the expression of Caspase-3 protein in HXO-RB44 cells was gradually increased with the increase of the concentration of SalB. CONCLUSION SalB can significantly affect on HXO-RB44 cells growth inhibition and apoptosis induction which may be achieved through the up-regulation of Caspase-3 expression and the induction of cell cycle arrest. PMID:22773971

  1. Differences in proximal (cardia) versus distal (antral) gastric carcinogenesis via retinoblastoma pathway

    Institute of Scientific and Technical Information of China (English)

    Christian Gulmann; Helen Hegarty; Antoinette Grace; Mary Leader; Stephen Patchett; Elaine Kay

    2004-01-01

    AIM: Disruption of cell cycle regulation is a critical event in carcinogenesis, and alteration of the retinoblastoma (pRb)tumour suppressor pathway is frequent. The aim of this study was to compare alterations in this pathway in proximal and distal gastric carcinogenesis in an effort to explain the observed striking epidemiological differences.METHODS: Immunohistochemistry was performed to investigate expression of p16 and pRb in the following groups of both proximal (cardia) and distal (antral) tissue samples: (a) biopsies showing normal mucosa, (b) biopsies showing intestinal metaplasia and, (c) gastric cancer resection specimens including uninvolved mucosa and tumour.RESULTS: In the antrum there were highly significant trends for increased p16 expression with concomitant (and in the group of carcinomas inversely proportional)decreased pRb expression from normal mucosa to intestinal metaplasia to uninvolved mucosa (from cancer resections)to carcinoma. In the cardia, there were no differences in p16 expression between the various types of tissue samples whereas pRb expression was higher in normal mucosa compared with intestinal metaplasia and tissue from cancer resections.CONCLUSION: Alterations in the pRb pathway appear to play a more significant role in distal gastric carcinogenesis.Tt may be an early event in the former location since the trend towards p16 overexpression with concomitant pRb underexpression was seen as early as between normal mucosa and intestinal metaplasia. Importantly, the marked differences in expression of pRb and p16 between the cardia and antrum strongly support the hypothesis that tumours of the two locations are genetically different which may account for some of the observed epidemiological differences.

  2. External beam irradiation for retinoblastoma: patterns of failure and dose-response analysis

    Energy Technology Data Exchange (ETDEWEB)

    Foote, R.L.; Garretson, B.R.; Schomberg, P.J.; Buskirk, S.J.; Robertson, D.M.; Earle, J.D.

    1989-03-01

    Eighteen children with retinoblastoma (25 eyes) were treated with external beam radiation at the Mayo Clinic between January 1977 and January 1987; 15 eyes were in groups I to III and 10 were in groups IV and V. The median number of tumors per eye was 3. Radiation therapy consisted of 4- or 6-MV photons. Doses varied from 39 to 51 Gy in 1.8- to 3.0-Gy fractions. Fourteen eyes were treated through lateral fields by anterior segment-sparing techniques, and 11 eyes were treated by an anterior approach with no attempt at anterior segment sparing. All patients survived (median follow-up, 31.5 months). Cataracts developed in five eyes at a median of 23 months, four in eyes treated with anterior segment-sparing techniques. Of the 15 group I to III eyes, 6 required additional treatment; 4 were salvaged with cryotherapy or photocoagulation and 2 were enucleated. Of the 10 group IV and V eyes, 8 required additional treatment; 4 were salvaged with cryotherapy or photocoagulation, 1 with persistent disease is being followed closely, and 3 were enucleated. Ten (71%) of the 14 eyes treated with anterior segment-sparing techniques required additional treatment. Four (36%) of the 11 eyes treated with an anterior approach required additional treatment. Ninety percent of the tumors 10 disc diameters or smaller (1 disc diameter = 1.6 mm) were controlled independently of dose and fractionation used when they were not in the low-dose area of the anterior retina of an eye treated with an anterior segment-sparing technique. We find that use of lateral, anterior segment-sparing techniques has a high risk of anterior retinal tumor development and cataract formation and should be abandoned in favor of techniques that treat the entire retina.

  3. Da antropologia à antropologia aplicada ou a afirmação da antropologia no Norte de Portugal

    OpenAIRE

    2014-01-01

    A antropologia aplicada diferenciou a antropologia no Norte de Portugal desde os anos 1990. No artigo realizamos uma análise desse processo de diferenciação e afirmação na oferta educativa do ensino superior do Norte de Portugal.

  4. Direito e Risco: do Consumo ao Ambiente

    OpenAIRE

    Marques, Carlos Alexandre Michaello

    2014-01-01

    A presente Dissertação tem como tema de investigação “Direito e Risco: do Consumo ao Ambiente”. Para tanto, essa temática foi delimitada através da investigação sobre a capacidade do Direito a partir de uma leitura Sociológica e Antropológica de compreender e promover aprendizagem nas questões que envolvem o Risco como elemento decorrente do Consumo e que externa suas consequências ao Ambiente. Neste sentido, se direcionou a responder ao problema de saber: em que medida é possível e necessári...

  5. La psicología aplicada en España

    OpenAIRE

    Sáiz Roca, Milagros; Sáiz Roca, Dolores

    1998-01-01

    Nuestro artículo ofrece una panorámica del inicio y desarrollo de la psicología aplicada en España. En él veremos cómo la aplicación de la psicología a los diferentes ámbitos -educativo, militar, jurídico, industrial, etc.- surgió en España de una forma casi paralela a otros países; sin embargo, tuvo una diferente demanda social y faltó su desarrollo en el seno de la universidad. Ante la ausencia de un marco universitario fueron los institutos de orientación profesional quienes generaron un p...

  6. Técnicas de investigación minera aplicadas a la Arqueología.

    OpenAIRE

    Iraizoz Fernández, José María; Octavio PUCHE RIART; Mansilla Plaza, Luis

    1988-01-01

    Hoy nos encontramos a las puertas de una nueva aplicación para una serie de técnicas mineras que hasta ahora habían tenido su máximo apogeo en una serie de actividades de carácter minero-económico principalmente. Nos estamos refiriendo al campo de la Arqueología, cuya aplicación ha hecho posible la aparición de nuevas ramas de la Ciencia Aplicada como la Arqueofísica, Teledetecci6n en arqueología, etc. A través de ellas se han logrado resultados espectaculares posibilitando una planificación...

  7. Cell death, chromosome damage and mitotic delay in normal human, ataxia telangiectasia and retinoblastoma fibroblasts after x-irradiation.

    Science.gov (United States)

    Zampetti-Bosseler, F; Scott, D

    1981-05-01

    We recently showed (Scott and Zampetti-Bosseler 1980) that X-ray sensitive mouse lymphoma cells sustain more chromosome damage, mitotic delay and spindle defects than X-ray resistant cells. We proposed that (a) chromosome aberrations contribute much more to lethality than spindle defects, and (b) that DNA lesions are less effectively repaired in the sensitive cells and give rise to more G2 mitotic delay and chromosome aberrations. Our present results on human fibroblasts with reported differential sensitivity to ionizing radiation (i.e. normal donors and patients with ataxia telangiectasia and retinoblastoma) support the first hypothesis since we observed a positive correlation between chromosome aberration frequencies and cell killing and no induced spindle defects. Our second hypothesis is however not substantiated since X-ray sensitive fibroblasts from the ataxia patient suffered less mitotic delay than cells from normal donors. A common lesion for mitotic delay and chromosome aberrations can still be assumed by adopting the hypothesis of Painter and Young (1981) that the defect in ataxia cells is not in repair but in a failure of DNA damage to initiate mitotic delay. In contrast to other reports, we found the retinoblastoma cells to be of normal radiation sensitivity (cell killing and aberration).

  8. A comprehensive, sensitive and economical approach for the detection of mutations in the RB1 gene in retinoblastoma

    Indian Academy of Sciences (India)

    Vidya Latha Parsam; Chitra Kannabiran; Santosh Honavar; Geeta K. Vemuganti; Mohammad Javed Ali

    2009-12-01

    Retinoblastoma (Rb) is the most common primary intraocular malignancy in children. It is brought about by the mutational inactivation of both alleles of RB1 gene in the developing retina. To identify the RB1 mutations, we analysed 74 retinoblastoma patients by screening the exons and the promoter region of RB1. The strategy used was to detect large deletions/duplications by fluorescent quantitative multiplex PCR; small deletions/insertions by fluorescent genotyping of RB1 alleles, and point mutations by PCR-RFLP and sequencing. Genomic DNA from the peripheral blood leucocytes of 74 Rb patients (53 with bilateral Rb, 21 with unilateral Rb; 4 familial cases) was screened for mutations. Recurrent mutations were identified in five patients with bilateral Rb, large deletions in 11 patients (nine with bilateral Rb and two with unilateral Rb), small deletions/insertions were found in 12 patients all with bilateral Rb, and point mutations in 26 patients (14 nonsense, six splice site, five substitution and one silent change). Three mutations were associated with variable expressivity of the disease in different family members. Using this method, the detection rates achieved in patients with bilateral Rb were 44/53 (83%) and with unilateral Rb, 5/21 (23.8%). This approach may be feasible for clinical genetic testing and counselling of patients.

  9. A retinoblastoma orthologue is a major regulator of S-phase, mitotic, and developmental gene expression in Dictyostelium.

    Directory of Open Access Journals (Sweden)

    Kimchi Strasser

    Full Text Available BACKGROUND: The retinoblastoma tumour suppressor, Rb, has two major functions. First, it represses genes whose products are required for S-phase entry and progression thus stabilizing cells in G1. Second, Rb interacts with factors that induce cell-cycle exit and terminal differentiation. Dictyostelium lacks a G1 phase in its cell cycle but it has a retinoblastoma orthologue, rblA. METHODOLOGY/PRINCIPAL FINDINGS: Using microarray analysis and mRNA-Seq transcriptional profiling, we show that RblA strongly represses genes whose products are involved in S phase and mitosis. Both S-phase and mitotic genes are upregulated at a single point in late G2 and again in mid-development, near the time when cell cycling is reactivated. RblA also activates a set of genes unique to slime moulds that function in terminal differentiation. CONCLUSIONS: Like its mammalian counterpart Dictyostelium, RblA plays a dual role, regulating cell-cycle progression and transcriptional events leading to terminal differentiation. In the absence of a G1 phase, however, RblA functions in late G2 controlling the expression of both S-phase and mitotic genes.

  10. Placas de aço inoxidável 316L aplicadas no reparo de fratura experimental diafisária do rádio e ulna de cães

    Directory of Open Access Journals (Sweden)

    Brasil F.B.J.

    2001-01-01

    Full Text Available O objetivo do presente trabalho foi o de estudar a resistência à corrosão em placas de aço inoxidável 316L, com diferentes tipos de acabamento e tratamento superficial, e a possível interferência dessa reação corrosiva na consolidação óssea. Utilizaram-se placas semi-acabadas, polidas, tratadas com jatos de microesferas de vidro e passivadas, as quais foram aplicadas na epífise distal do rádio de cães. Foram utilizados 12 animais, divididos em dois grupos, nos quais, após osteotomia bilateral do rádio e ulna, foram realizadas osteossínteses do rádio, totalizando 24 procedimentos. Avaliou-se a evolução clínica e radiográfica das regiões que receberam os implantes aos 30, 60, 90, 180, 240 e 360 dias. Os animais do grupo 1 (GI foram sacrificados aos 180 dias e os do GII aos 360 dias para estudo histológico e de microscopia eletrônica de varredura do local da osteotomia sob a região dos implantes metálicos e para estudo da resistência à corrosão no organismo, pelos implantes metálicos, por meio de análises química e metalográfica (microscopia óptica e eletrônica de varredura e espectroscopia de espalhamento de energia por raios X. Os animais recuperaram a função dos membros operados 24 horas após a cirurgia. Radiograficamente, verificou-se a consolidação óssea em todos os animais. Macro e microscopicamente não foram observados sinais de corrosão nos implantes metálicos, exceto em uma placa passivada, aplicada no rádio esquerdo de um animal, na qual a corrosão foi detectada pela microscopia óptica e eletrônica de varredura. Este estudo permite concluir que as placas de aço inoxidável 316L, independente do acabamento superficial a que foram submetidas, não sofreram corrosão ou reações adversas e foram efetivas no tratamento das fraturas experimentais do rádio e ulna de cães.

  11. Modelagem molecular aplicada ao desenvolvimento de moléculas com atividade antioxidante visando ao uso cosmético Molecular modeling applied to the development of molecules with antioxidant activity for cosmetic use

    Directory of Open Access Journals (Sweden)

    Luciana Scotti

    2007-06-01

    Full Text Available Várias hipóteses e constantes estudos são realizados com o intuito de elucidar o envelhecimento cutâneo. Para prevenir e contornar este processo torna-se necessário reforçar nossas defesas naturais antioxidantes endógenas. Diversas substâncias antioxidantes exógenas, como vitaminas, extratos vegetais, dentre outras, são utilizadas pela Cosmetologia nos produtos antienvelhecimento. O objetivo deste artigo é apresentar de que forma a Modelagem Molecular pode ser uma ferramenta útil na pesquisa por novas substâncias cosméticas antioxidantes para combater o envelhecimento cutâneo.Some hypotheses and constants studies are made with intention to elucidate the aging process. To prevent and to attenuate the cutaneous aging it becomes necessary to strengthen our endogenous antioxidant natural defenses. Diverse exogenous antioxidant substances, as vitamins, vegetal extracts and others, have been used by the Cosmetology in antiaging products. The objective of this paper is to show how the Molecular Modeling can be an useful tool in the research for new antioxidant cosmetic substances to face the cutaneous aging.

  12. High-resolution MRI using orbit surface coils for the evaluation of metastatic risk factors in 143 children with retinoblastoma. Part 1: MRI vs. histopathology

    Energy Technology Data Exchange (ETDEWEB)

    Sirin, Selma; Schlamann, Marc; Schweiger, Bernd; Goericke, Sophia L. [University Hospital Essen, Department of Diagnostic and Interventional Radiology and Neuroradiology, Essen (Germany); Metz, Klaus A. [University Hospital Essen, Department of Pathology and Neuropathology, Essen (Germany); Bornfeld, Norbert; Holdt, Markus [University Hospital Essen, Department of Ophthalmology, Essen (Germany); Temming, Petra; Schuendeln, Michael M. [University Hospital Essen, Department of Pediatric Hematology and Oncology, Essen (Germany)

    2015-08-15

    A reliable detection of metastatic risk factors is important for children with retinoblastoma to choose the right therapeutic regimen. First studies using high-resolution magnetic resonance imaging (MRI) with orbit surface coils were promising. The aim of this study was therefore to evaluate the ability of high-resolution MRI to detect metastatic and especially advanced metastatic risk factors in a large group of children with retinoblastoma. One hundred forty-three consecutive children with retinoblastoma (148 enucleated eyes, 64 girls, 79 boys, mean age 19.7 ± 15.3) who received pretherapeutical high-resolution MRI with orbit surface coils on 1.5 T MR scanner systems between 2007 and 2012 and subsequent primary enucleation within 14 days were included in this retrospective study. Image analysis was performed by two neuroradiologists experienced in ocular imaging in consensus. Histopathology served as gold standard. Sensitivity/specificity for the detection of metastatic risk factors using high-resolution MRI with orbit surface coils were 60 %/88.7 % for postlaminar optic nerve infiltration, 65.5 %/95.6 % for choroidal invasion, 100 %/99.3 % for scleral invasion, and 100 %/100 % for peribulbar fat invasion, respectively. The results increased for the detection of advanced metastatic risk factors, 81.8 %/89.1 % for deep postlaminar optic nerve infiltration, 70.6 %/97.6 % for massive choroidal invasion. High-resolution MRI is clinically valuable for the detection of metastatic, especially of advanced metastatic risk factors in children with retinoblastoma. (orig.)

  13. Estudos recentes em Linguística Aplicada no Brasil a respeito de livros didáticos de língua estrangeira A review of recent studies on foreign language coursebooks in Applied Linguistics in Brazil

    Directory of Open Access Journals (Sweden)

    Renato Caixeta da Silva

    2010-01-01

    Full Text Available Este trabalho focaliza pesquisas brasileiras sobre livros didáticos (LD de língua estrangeira em Linguística Aplicada. Considera-se o lugar ocupado na área por pesquisas sobre material didático para o ensino de línguas estrangeiras (Coracini, 1999; Fracalanza; Santoro, 1989; Moita Lopes, 1999; dentre outros, e é apresentado um panorama das pesquisas em Linguística Aplicada no Brasil envolvendo o LD de línguas estrangeiras no período de 1998 a 2008, mostrando os temas enfocados mais recentemente nestas pesquisas. Para tal, foram consultadas páginas da internet dos programas de pós-graduação em Linguística Aplicada, Estudos da Linguagem ou Línguas Estrangeiras de universidades brasileiras, bem como revistas especializadas na área, e, por fim, foi considerada a realização do I SILID e II SILID/ISIMAR na PUC-Rio, eventos específicos sobre o LD de línguas. Ao final, são apresentados novos temas e tendências de pesquisas, as implicações de investigações envolvendo LD de línguas estrangeiras e a necessidade de diálogos entre pesquisadores e professores (Kramsch, 1995; Coracini, 1999.The focus of this paper is foreign language coursebook studies in Applied Linguistics in Brazil. The role such research plays in the field is considered. (Coracini, 1999; Fracalanza; Santoro, 1989; Moita Lopes, 1999; among others and an overview of Brazilian studies developed between 1998-2008 is outlined, in an attempt to show the most recent focused themes. In order to do that some websites of Graduate Programs in Applied Linguistics, Language Studies and Foreign Language at Brazilian universities were accessed as well as some journals in the field, and also two specific events on language coursebooks were considered - I SILID and II SILID / I SIMAR at PUC-Rio. We conclude by suggesting new themes and trends for future studies and the need for dialogue between researchers and teachers (Kramsch, 1995; Coracini, 1999

  14. A teoria das representações sociais como referencial didático-metodológico de pesquisa no campo das ciências humanas e sociais aplicadas

    Directory of Open Access Journals (Sweden)

    Marcos Pereira dos Santos

    2012-11-01

    Full Text Available Este artigo tem como principal objetivo efetuar reflexões acerca da Teoria das Representações Sociais (TRS de Serge Moscovici como referencial didático-metodológico de pesquisa no campo das Ciências Humanas e Sociais Aplicadas. Para tanto, inicialmente são apresentadas algumas definições conceituais gerais que gravitam em torno da palavra representação(ões, bem como uma breve visão panorâmica da Teoria das Representações Sociais. Em seguida, busca-se tecer comentários sobre a abordagem estrutural (ou do núcleo central de Jean-Claude Abric como suporte teórico e metodológico das representações sociais. Por fim, trazemos à discussão questões concernentes à utilização da Teoria das Representações Sociais no desenvolvimento de pesquisas científicas e sua importância na atualidade. O estudo realizado apontou que a Teoria das Representações Sociais configura-se como um importante instrumento didático-metodológico de pesquisa científica e compreensão de uma determinada realidade existencial pertinente ao campo das Ciências Humanas e Sociais Aplicadas, uma vez que faz emergir concepções, discursos, reflexões e significados sobre diferentes fenômenos resultantes de experiências e relações concretas vivenciadas pelos sujeitos sociais no cotidiano.

  15. Desenvolvimento de habilidades informacionais: um estudo das atividades de educação de usuários aplicadas na Biblioteca do Colégio Israelita

    Directory of Open Access Journals (Sweden)

    Regina Dioga Pelissaro

    2015-06-01

    Full Text Available Introdução: Para atingir a competência informacional é preciso aprender habilidades de busca e uso da informação, que devem começar a ser desenvolvidas desde cedo através das atividades de educação de usuários aplicadas nas bibliotecas escolares. Avaliar as ações de educação de usuários é válido para constatar se as crianças internalizam os conhecimentos que lhes são repassados.Objetivo: Analisar a relação das habilidades desenvolvidas pelas crianças de 1º ano do Ensino Fundamental do Colégio Israelita de Porto Alegre/RS, com as habilidades propostas pela autora Kuhlthau para a fase I, 1ª etapa, correspondente à idade dos quatro aos seis anos. Metodologia: Os dados foram coletados por observação e grupo focal e foram analisados qualitativamente. Resultados: Os resultados indicam que as crianças possuem a maioria das habilidades propostas pela autora e aquelas que não estão completamente desenvolvidas podem ser melhoradas através das atividades propostas neste estudo e, outras, no final do ano, quando completarem a alfabetização.Conclusões: O estudo considera as avaliações essenciais, pois através delas se constata se os alunos adquiriram as habilidades praticadas nas ações de educação de usuários.

  16. Clinical treatment and prognostic observation for different pathological infiltrations in 537 patients with unilateral retinoblastoma

    Institute of Scientific and Technical Information of China (English)

    Wang Yizhuo; Huang Dongsheng; Shi Jitong; Ma Jianmin; Zhao Junyang; Li Bin; Xu Xiaolin

    2014-01-01

    Background The use of post-enucleation adjuvant therapy to decrease the extraocular relapse rate is frequently considered,but there is much controversy about the indications for adjuvant therapy.The aim of this retrospective study was to observe the treatment and prognosis for different degrees of invasion of eye tissue in retinoblastoma (RB) and identify the indications for post-enucleation adjuvant therapy.Methods We recruited 537 children who had been diagnosed with unilateral RB and had received enucleation from January 2006 to December 2012 in our hospital,and divided them into three groups according to their number of histopathologic risk factors:0 factor,1 factor,or >2 factors.Histopathologic high-risk factors included invasion of the optic nerve posterior to the ethmoid plate (including optic nerve stumps) and extensive invasions of the choroid,sclera,anterior chamber,iris,and ciliary body.Treatment was delivered accordingly,and the prognosis of different degrees of histopathologic invasion was observed.The subjects were followed up for 6 months to 7 years (average follow-up time:35 months).Statistical analysis was analyzed using x2test.P <0.05 was considered significant.Results Of the 537 RB patients who received enucleation,25 died (overall survival:95.3%).Of the 369 (68.7%) with no histopathologic risk factors,1 died of recurrence,with a mortality rate of 0.3%,whereas of the 168 (31.3%) with histopathologic risk factors,26 had recurrences and 24 died (mortality rate:14.3%; P=0.000).Of the 93 patients (17.3%),each of whom had a single risk factor,nine had recurrences,16 died (8.6%).Of the 75 patients (14%) with two or more high-risk factors,16 died (21.3%).These differences were statistically significant between the three (P=0.000).Conclusion Chemotherapy is recommended for patients with histopathologic risk factors,especially those with two or more histopathologic risk factors.

  17. The Experiment Research of Subconjunctival Injections with Etoposide for the Treatment of Retinoblastoma Mice

    Institute of Scientific and Technical Information of China (English)

    Yuxiang Mao; Zhongyao Wu; Huasheng Yang; Jianliang Zheng; Jianfeng He; Wei Li

    2005-01-01

    Purpose: To evaluate the efficacy of subconjunctival injections of Etoposide for retinoblastoma(RB) mice.Method: Twenty-five RB mice were divided into 5 groups for the study(G0~G4). GO for control group received 0.9% sodium chloride injection subconjunctival. G1: 5 RB mice for systemic delivery received 6 systemic administration of Etoposide 150 μg and Cyclosporin A (CSA)1.0mg at 3 days intervals, respectively. G2~G4:5 RB mice in each group received 6 subconjunctival delivery of Etoposide in 50、75、150μg at 3 days intervals, respectively.To observe the changes of the volume of RB in anterior chamber and the part of conjunctiva for injections of chemotherapeutic drugs. All eyes and conjunctiva and scleras of the part for drugs injections were obtained at 6 weeks after experiment for histopathologic examination. The findings under the light microscope were analysed with software to measure the areas of RB in anterior chambers and the tumor inhibition rate.Results:The volume of RB in anterior chambers for G1 and G2 increase quickly and none of the mice exhibited tumor control. The RB volume increase slowly in G3 and exhibit tumor control in G4. The areas of tumors in G0~G4 are 0.710,0.659,0.634,0.515,0.235 mm2, respectively. The rate for tumor inhibition in G0~G4 are 0.0%,7.18%, 10.70% ,27.43% ,66.90%, respectively. There are statistical significant difference between the G1 and G4 for the volumes of tumors and the rate of tumor inhibition.The pathologic examination of subconjunctiva and sclera for the injective sites showed no pathologic changes.Conclusions: There are significant effects for the method of subconjunctival delivery of Etoposide in RB control, demonstrating a positive dose-related relationship. Subconjunctival injection of Etoposide reached better efficacy in controlling and inhibiting RB cells than systemic delivery of Etoposide for the same dose. The pathologic results showed the subconjunctival delivery of etoposide were safety for

  18. Health-related quality of life of child and adolescent retinoblastoma survivors in the Netherlands

    Directory of Open Access Journals (Sweden)

    Bezemer Pieter D

    2007-12-01

    Full Text Available Abstract Background To assess health-related quality of life (HRQoL in children (8–11 years and adolescents (12–18 years who survived retinoblastoma (RB, by means of the KIDSCREEN self-report questionnaire and the proxy-report version. Methods This population-based cross-sectional study (participation rate 70% involved 65 RB survivors (8–18 years and their parents. Child/adolescents' and parents' perception of their youth's HRQoL was assessed using the KIDSCREEN, and the results were compared with Dutch reference data. Relations with gender, age, marital status of the parents, and visual acuity were analyzed. Results RB survivors reported better HRQoL than did the Dutch reference group on the dimensions "moods and emotions" and "autonomy". Increased ratings of HRQoL in RB survivors were mainly seen in perceptions of the younger children and adolescent girls. RB survivors with normal visual acuity scored higher on "physical well-being" than visually impaired survivors. Age was negatively associated with the dimensions "psychological well-being", "self-perception" (according to the child and parent reports and "parent relations and home life" (according to the child. "Self-perception" was also negatively associated with visual acuity (according to the child. Only parents of young boys surviving RB reported lower on "autonomy" than the reference group, and parents of low visual acuity and blind RB survivors reported higher on "autonomy" than parents of visually unimpaired survivors. Survivors' perceptions and parents' perceptions correlated poorly on all HRQoL dimensions. Conclusion RB survivors reported a very good HRQoL compared with the Dutch reference group. The perceptions related to HRQoL differ substantially between parents and their children, i.e. parents judge the HRQoL of their child to be relatively poorer. Although the results are reassuring, additional factors of HRQoL that may have more specific relevance, such as psychological

  19. Alergia ao látex

    Directory of Open Access Journals (Sweden)

    Allarcon Jorge Barrios

    2003-01-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O látex, (cis 1,4 polyisoprene está muito presente no dia-a-dia do anestesiologista. Os primeiros relatos de alergia ao látex datam de 1933. A sensibilidade ao látex na população de anestesiologistas é de 12,5% a 15,8%. A incidência de sensibilidade ao látex aumenta muito em grupos específicos, podendo produzir reações graves e até mesmo fatais. O objetivo desse estudo foi realizar uma revisão bibliográfica sobre o assunto, abordando este tema de interesse para o anestesiologista, considerando diagnóstico, prevenção e tratamento da alergia ao látex. CONTEÚDO: Os estudos sobre a prevalência das reações alérgicas ao látex variam muito conforme a população estudada e os métodos utilizados para estabelecer a sensibilidade ao antígeno. Mesmo com estas variações, existem alguns grupos em que o risco é elevado, particularmente em pacientes com distúrbio na formação do tubo neural. As manifestações clínicas variam desde dermatite de contato até anafilaxia e óbito. O diagnóstico precoce com pesquisa de IgE pode prevenir as reações de hipersensibilidade. CONCLUSÕES: É de extrema importância o diagnóstico precoce da alergia ao látex. A abordagem desses pacientes em ambientes livres de látex evitam a sensibilização e evolução muitas vezes para quadros dramáticos.

  20. Diffuse infiltrating retinoblastoma simulating endophthalmitis: a case report%以眼内炎为首发症状的弥漫浸润型视网膜母细胞瘤1例

    Institute of Scientific and Technical Information of China (English)

    董凯; 何为民; Darius M. Moshfeghi; 徐筑萍

    2008-01-01

    弥漫浸润型视网膜母细胞瘤是视网膜母细胞瘤中一种少见的病理类型,发病率约为视网膜母细胞瘤的1%~2%,通常会由于检查不到明显的肿块而误诊为内源性眼内炎,本文报道1例以眼内炎为首发症状的弥漫浸润型视网膜母细胞瘤,并对其组织病理进行探讨.%·Diffusely infiltrating retinoblastoma is a rare histopatho-logic type of retinoblastoma, accounting for approximate-ly 1% to 2% of retinoblastoma cases. We report a case of diffuse infiltrating retinoblastoma presenting as endo-phthalmitis and comment on the histopathologic findings.

  1. Transcriptional regulatory program in wild-type and retinoblastoma gene-deficient mouse embryonic fibroblasts during adipocyte differentiation

    Directory of Open Access Journals (Sweden)

    Hansen Jacob B

    2011-05-01

    Full Text Available Abstract Background Although many molecular regulators of adipogenesis have been identified a comprehensive catalogue of components is still missing. Recent studies showed that the retinoblastoma protein (pRb was expressed in the cell cycle and late cellular differentiation phase during adipogenesis. To investigate this dual role of pRb in the early and late stages of adipogenesis we used microarrays to perform a comprehensive systems-level analysis of the common transcriptional program of the classic 3T3-L1 preadipocyte cell line, wild-type mouse embryonic fibroblasts (MEFs, and retinoblastoma gene-deficient MEFs (Rb-/- MEFs. Findings Comparative analysis of the expression profiles of 3T3-L1 cells and wild-type MEFs revealed genes involved specifically in early regulation of the adipocyte differentiation as well as secreted factors and signaling molecules regulating the later phase of differentiation. In an attempt to identify transcription factors regulating adipogenesis, bioinformatics analysis of the promoters of coordinately and highly expressed genes was performed. We were able to identify a number of high-confidence target genes for follow-up experimental studies. Additionally, combination of experimental data and computational analyses pinpointed a feedback-loop between Pparg and Foxo1. To analyze the effects of the retinoblastoma protein at the transcriptional level we chose a perturbated system (Rb-/- MEFs for comparison to the transcriptional program of wild-type MEFs. Gene ontology analysis of 64 deregulated genes showed that the Rb-/- MEF model exhibits a brown(-like adipocyte phenotype. Additionally, the analysis results indicate a different or additional role for pRb family member involvement in the lineage commitment. Conclusion In this study a number of commonly modulated genes during adipogenesis in 3T3-L1 cells and MEFs, potential transcriptional regulation mechanisms, and differentially regulated targets during adipocyte

  2. Advanced retinoblastoma treatment: targeting hypoxia by inhibition of the mammalian target of rapamycin (mTOR in LHBETATAG retinal tumors

    Directory of Open Access Journals (Sweden)

    Y Piña

    2011-03-01

    significantly different between both groups (P = 0.092.Conclusion: Inhibition of mTOR was shown to reduce tumor burden, hypoxia, and vasculature in the LHBETATAG retinoblastoma tumor model. Rapamycin may have a role in combination with chemotherapy or other adjuvant therapies to enhance retinoblastoma tumor control.Keywords: rapamycin, mTOR, hypoxia, retinoblastoma, anaerobic glycolysis

  3. Resistência ao cisalhamento entre ligas metálicas e materiais compostos para revestimento estético: cerômero e polímero de vidro

    OpenAIRE

    Itinoche,Marcos Koiti; Kimpara, Estevão Tomomitsu; Bottino,Marco Antonio; Neisser, Maximiliano Piero; Oyafuso,Denise Kanashiro

    2010-01-01

    O propósito deste trabalho foi avaliar a resistência ao cisalhamento entre ligas metálicas (Au, NiCr e CoCr) e materiais estéticos indiretos (Artglass e Targis). Para tanto, utilizaram-se vinte estruturas metálicas de cada tipo de liga, cuja superfície a ser aplicada os materiais estéticos, receberam jateamento com óxido de alumínio de granulação de 250mm, antes da aplicação do sistema adesivo do corresp...

  4. Reacções psicológicas e estratégias de coping parentais, face ao diagnóstico de perturbação do espectro do autismo, de um filho

    OpenAIRE

    2008-01-01

    Dissertação de Mestrado apresentada ao Instituto Superior de Psicologia Aplicada para obtenção de grau de Mestre na especialidade de Psicologia Clínica. Os Transtornos Globais do Desenvolvimento (TGD) caracterizam-se pelo comprometimento da interacção social, pelo uso estereotipado da linguagem e pela presença de comportamentos repetitivos com interesses restritos. O presente estudo objectivou investigar se o tempo de diagnóstico de perturbação do espectro do autismo, de um fil...

  5. Análise da convergência e harmonização das normas brasileiras de contabilidade (BR GAAP) com as normas internacionais de contabilidade relativas ao patrimônio líquido

    OpenAIRE

    Maria de Lurdes Furno da Silva

    2006-01-01

    Esta dissertação apresenta análise da convergência e harmonização das normas brasileiras de contabilidade, BR GAAP, com as normas internacionais de contabilidade IFRS – International Financial Reporting Standards, estabelecidas pelo IASB - International Accounting Standards Board, referentes à contabilização de operações, preparação e divulgação das demonstrações contábeis relacionadas ao patrimônio líquido. Tem como base o comparativo entre as normas contábeis aplicadas no Brasil e na Comuni...

  6. Estudo do raspado de conjuntiva e margem palpebral de pacientes tratados de retinoblastoma: etapa I - Microflora aeróbica Study of the conjunctival scrape and eyelid margin smears in patients treated for retinoblastoma: phase I - Aerobic microflora

    Directory of Open Access Journals (Sweden)

    Maria Carmen Menezes Santos

    2002-03-01

    Full Text Available Objetivo: A queixa de secreção ocular é muito freqüente entre os pacientes tratados de retinoblastoma submetidos à enucleação e radioterapia. A falta de dados sobre a microflora em pacientes nessas condições foi o que motivou este estudo. Métodos: Foram examinadas 114 órbitas de 58 pacientes. Este trabalho apresenta os resultados das culturas para bactérias aeróbicas e fungos, de conjuntiva e margem palpebral em 4 condições, com e sem enucleação, com e sem radioterapia. Procedeu-se à identificação dos microrganismos de acordo com o Protocolo do National Committee for Clinical Laboratory Standards e os esfregaços para citologia foram submetidos às colorações de Gram e Leishman. Resultados: A presença de microrganismos foi mais freqüente nas cavidades anoftálmicas irradiadas, tanto na conjuntiva (54,5% quanto na margem palpebral (63,6%, porém sem diferença estatisticamente significante. Além disso, em órbitas com bulbo ocular, a radiação, na fase tardia, representou um fator limitante da presença de microrganismos na conjuntiva e na margem palpebral, mas sem diferença estatisticamente significante. O Staphylococcus coagulase negativa foi o microrganismo mais freqüente na conjuntiva e margem palpebral de todos os grupos. Houve crescimento de fungo, Candida tropicalis, em apenas um caso. Conclusões: Sugere-se o uso de colírios antibióticos em pacientes tratados de retinoblastoma, portadores de cavidade anoftálmica, irradiada ou não.Purpose: Conjunctival discharge is a common complaint among patients treated for retinoblastoma who underwent enucleation and radiotherapy. Lack of data about the microflora in those patients motivated this study. Methods: One hundred and fourteen orbits of 58 patients were examined. The authors present the results of aerobic bacterial and fungal culture of conjunctival and eyelid margin smears, as well as conjunctival cytology, in 4 different conditions: with and without

  7. Lingüística aplicada e o ensino de línguas estrangeiras no Brasil

    Directory of Open Access Journals (Sweden)

    John Robert Schmitz

    2001-02-01

    Full Text Available

    Procura-se neste trabalho apresentar um esboço de Lingüística Aplicada e o Ensino de Línguas Estrangeiras no mundo e também no Brasil. Conclui-se que a disciplina está em pleno desenvolvimento com contribuições pertinentes para o ensino e aprendizagem de línguas, além de outras áreas de conhecimento tais como língua pátria, ensino bilíngüe, tradução, alfabetização e letramento. Destaca-se no artigo o estado da arte em Lingüística Aplicada no Brasil que atinge no momento, especialmente no campo de ensino de línguas estrangeiras, a sua maturidade após vinte anos de atividade.

  8. Revis\\~ao da Constru\\c{c}\\~ao de Modelos Supersim\\'etricos

    CERN Document Server

    Rodríguez, M C

    2001-01-01

    Foi com base neste estudo que fizemos a constru\\c{c}\\~ao da vers\\~ao supersim\\'etrica dos modelos de simetria $SU(3)_{C} \\otimes SU(3)_{L} \\otimes U(1)_{N}$ \\cite{susy331}, apresentado no final da minha tese de doutorado \\cite{mcr1}. Bem como dos estudos fenomenol\\'ogicos subsequente \\cite{mcr}.

  9. Into the Blue: AO Science with MagAO in the Visible

    CERN Document Server

    Close, Laird M; Follette, Katherine B; Hinz, Phil; Morzinski, Katie M; Wu, Ya-Lin; Kopon, Derek; Riccardi, Armando; Esposito, Simone; Puglisi, Alfio; Pinna, Enrico; Xompero, Marco; Briguglio, Runa; Quiros-Pacheco, Fernando

    2014-01-01

    We review astronomical results in the visible ({\\lambda}<1{\\mu}m) with adaptive optics. Other than a brief period in the early 1990s, there has been little astronomical science done in the visible with AO until recently. The most productive visible AO system to date is our 6.5m Magellan telescope AO system (MagAO). MagAO is an advanced Adaptive Secondary system at the Magellan 6.5m in Chile. This secondary has 585 actuators with < 1 msec response times (0.7 ms typically). We use a pyramid wavefront sensor. The relatively small actuator pitch (~23 cm/subap) allows moderate Strehls to be obtained in the visible (0.63-1.05 microns). We use a CCD AO science camera called "VisAO". On-sky long exposures (60s) achieve <30mas resolutions, 30% Strehls at 0.62 microns (r') with the VisAO camera in 0.5" seeing with bright R < 8 mag stars. These relatively high visible wavelength Strehls are made possible by our powerful combination of a next generation ASM and a Pyramid WFS with 378 controlled modes and 1000...

  10. Haploinsufficiency of the retinoblastoma protein gene reduces diet-induced obesity, insulin resistance, and hepatosteatosis in mice

    DEFF Research Database (Denmark)

    Mercader, Josep; Ribot, Joan; Murano, Incoronata

    2009-01-01

    ) on the response to high fat diet feeding in mice. Rb(+/-) mice had body weight and adiposity indistinguishable from that of wild-type (Rb(+/+)) littermates when maintained on a standard diet, yet they gained less body weight and body fat after long-term high fat diet feeding, coupled to reduced feed efficiency...... and increased rectal temperature. Rb haploinsufficiency ameliorated insulin resistance and hepatosteatosis after high fat diet in male mice, in which these disturbances were more marked than in females. Compared to wild-type littermates Rb(+/-) mice fed a high fat diet displayed higher expression of peroxisome......Brown adipose tissue activity dissipates energy as heat, and there is evidence that lack of the retinoblastoma protein (pRb) may favor the development of the brown adipocyte phenotype in adipose cells. In this work we assessed the impact of germ-line haploinsufficiency of the pRb gene (Rb...

  11. Experimental research on wild-type p53 plasmid transfected into retinoblastoma cells and tissues using an ultrasound microbubble intensifier.

    Science.gov (United States)

    Luo, J; Zhou, X; Diao, L; Wang, Z

    2010-01-01

    The transfection efficiency of wild-type p53 (wtp53) was investigated in retinoblastoma (RB) Y79 cells using an ultrasound microbubble technique. A human RB nude mouse xenograft tumour model was also used to investigate whether this technique could deliver wtp53 into solid tumours. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that wtp53 was successfully transfected into Y79 cells in the plasmid with microbubbles and ultrasound group and in the plasmid with liposomes group, but not in the plasmid with ultrasound group or in the untreated control group. Flow cytometry showed that apoptosis was highest in the microbubbles and ultrasound group (25.58%) compared with the plasmid with liposomes group (19.50%), and the other two groups (microbubbles and ultrasound group. This study provides preliminary evidence in support of a potential new approach to RB gene therapy.

  12. Retinoblastoma and p16 proteins in mammary carcinoma: their relationship to cyclin D1 and histopathological parameters.

    Science.gov (United States)

    Dublin, E A; Patel, N K; Gillett, C E; Smith, P; Peters, G; Barnes, D M

    1998-02-20

    The cell cycle-associated retinoblastoma protein (pRb) and p16 protein were demonstrated using immuno-histochemistry on paraffin sections from 192 cases of invasive breast carcinoma. Abnormal expression of pRb was defined as negative staining and was seen in 17% of tumours. Such abnormal expression was significantly more frequent in tumours with negative oestrogen receptor (ER) status. There was also a trend for tumours which were negative for pRb to be grade III ductal carcinomas. There was no association between p16 staining and any histopathological parameter, though, surprisingly, log-rank analysis showed that strong staining was associated with a poor outcome. There was a significant inverse relationship between pRb and p16 expression and a significant positive association between pRb and cyclin D1. In a Cox multivariate analysis, which included cyclin D1, neither pRb nor p16 was an independent predictor of patient outcome.

  13. Retinoblastoma protein expression is an independent predictor of both radiation response and survival in muscle-invasive bladder cancer

    DEFF Research Database (Denmark)

    Agerbaek, M; Alsner, J; Marcussen, N;

    2003-01-01

    The objective of the study was to investigate the predictive value of various clinical, biochemical, and histopathological parameters, with special emphasis on the expression of the retinoblastoma protein (pRB), on the radiation response in bladder cancer. In order to obtain a truly objective....... Expression of pRB was assessed by immunohistochemical staining as present or absent. Complete response to radiotherapy was obtained in 42 of 106 evaluable patients (40%). Predictive for CR to radiotherapy, in univariate analysis, was transurethral resection (as opposed to biopsy), B-haemoglobin, no upper...... urinary retention, and loss of pRB staining. Loss of pRB staining was the strongest independent predictor of radiation response in multivariate logistic regression analysis and absence of upper urinary retention was the only other significant factor. Loss of pRB was the only parameter showing...

  14. Descripción matemática de la función gompertz aplicada al crecimiento de animales

    Directory of Open Access Journals (Sweden)

    C. Manrique

    2010-04-01

    Full Text Available Desde su aparición en 1825, la curva sigmoidea propuesta por Benjamin Gompertzha sido aplicada en diferentes campos. En ciencias animales se usa frecuentementepara describir el crecimiento de los individuos. En el presente trabajo se muestra unanálisis matemático de la función y la construcción de algunos de los parámetros quese obtienen de la misma.

  15. DESCRIPCIÓN MATEMÁTICA DE LA FUNCIÓN GOMPERTZ APLICADA AL CRECIMIENTO DE ANIMALES

    Directory of Open Access Journals (Sweden)

    C. A. Martínez

    2010-01-01

    Full Text Available Desde su aparición en 1825, la curva sigmoidea propuesta por Benjamin Gompertz ha sido aplicada en diferentes campos. En ciencias animales se usa frecuentemente para describir el crecimiento de los individuos. En el presente trabajo se muestra un análisis matemático de la función y la construcción de algunos de los parámetros que se obtienen de la misma.

  16. Descripción matemática de la función gompertz aplicada al crecimiento de animales

    OpenAIRE

    2010-01-01

    Desde su aparición en 1825, la curva sigmoidea propuesta por Benjamin Gompertzha sido aplicada en diferentes campos. En ciencias animales se usa frecuentementepara describir el crecimiento de los individuos. En el presente trabajo se muestra unanálisis matemático de la función y la construcción de algunos de los parámetros quese obtienen de la misma.

  17. Descripción matemática de la función gompertz aplicada al crecimiento de animales

    OpenAIRE

    2010-01-01

    Desde su aparición en 1825, la curva sigmoidea propuesta por Benjamin Gompertz ha sido aplicada en diferentes campos. En ciencias animales se usa frecuentemente para describir el crecimiento de los individuos. En el presente trabajo se muestra un análisis matemático de la función y la construcción de algunos de los parámetros que se obtienen de la misma.

  18. Expertos culturales e intervención social: tensiones y transformaciones en antropología aplicada

    Directory of Open Access Journals (Sweden)

    Enrique Jaramillo Buenaventura

    2008-12-01

    Full Text Available A partir de una breve revisión bibliográfica sobre la antropología aplicada y la antropología del desarrrollo este artículo explora algunos de los problemas teriricos y metodológicos sobre los retos y las implicaciones de pensar a la antropología como un "saber experto" parala intervención social.

  19. Twelve thousand laser-AO observations: first results from the Robo-AO large surveys

    Science.gov (United States)

    Law, Nicholas M.; Baranec, Christoph; Riddle, Reed L.

    2014-07-01

    Robo-AO is the first AO system which can feasibly perform surveys of thousands of targets. The system has been operating in a fully robotic mode on the Palomar 1.5m telescope for almost two years. Robo-AO has completed nearly 12,000 high-angular-resolution observations in almost 20 separate science programs including exoplanet characterization, field star binarity, young star binarity and solar system observations. We summarize the Robo-AO surveys and the observations completed to date. We also describe the data-reduction pipeline we developed for Robo-AO—the first fully-automated AO data-reduction, point-spread-function subtraction and companion-search pipeline.

  20. The Modern Role of Radiation Therapy in Treating Advanced-Stage Retinoblastoma: Long-Term Outcomes and Racial Differences

    Energy Technology Data Exchange (ETDEWEB)

    Orman, Amber [Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, Florida (United States); Koru-Sengul, Tulay [Department of Public Health Sciences, University of Miami Miller School of Medicine, Miami, Florida (United States); Miao, Feng [Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, Florida (United States); Markoe, Arnold [Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, Florida (United States); Panoff, Joseph E., E-mail: jpanoff@med.miami.edu [Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, Florida (United States)

    2014-12-01

    Purpose/Objective(s): To evaluate the effects of various patient characteristics and radiation therapy treatment variables on outcomes in advanced-stage retinoblastoma. Methods and Materials: This was a retrospective review of 41 eyes of 30 patients treated with external beam radiation therapy between June 1, 1992, and March 31, 2012, with a median follow-up time of 133 months (11 years). Outcome measures included overall survival, progression-free survival, local control, eye preservation rate, and toxicity. Results: Over 90% of the eyes were stage V. Definitive external beam radiation therapy (EBRT) was delivered in 43.9% of eyes, adjuvant EBRT in 22% of eyes, and second-line/salvage EBRT in 34.1% of eyes. A relative lens sparing (RLS) technique was used in 68.3% of eyes and modified lens sparing (MLS) in 24.4% of eyes. Three eyes were treated with other techniques. Doses ≥45 Gy were used in 68.3% of eyes. Chemotherapy was a component of treatment in 53.7% of eyes. The 10-year overall survival was 87.7%, progression-free survival was 80.5%, and local control was 87.8%. White patients had significantly better overall survival than did African-American patients in univariate analysis (hazard ratio 0.09; 95% confidence interval 0.01-0.84; P=.035). Toxicity was seen in 68.3% of eyes, including 24.3% with isolated acute dermatitis. Conclusions: External beam radiation therapy continues to be an effective treatment modality for advanced retinoblastoma, achieving excellent long-term local control and survival with low rates of treatment-related toxicity and secondary malignancy.

  1. Enhanced in vitro antiproliferative effects of EpCAM antibody-functionalized paclitaxel-loaded PLGA nanoparticles in retinoblastoma cells

    Science.gov (United States)

    Mitra, Moutushy; Misra, Ranjita; Harilal, Anju; Sahoo, Sanjeeb K

    2011-01-01

    Background To specifically deliver paclitaxel (PTX) to retinoblastoma (RB) cells, the anionic surface-charged poly(lactic-co-glycolic acid) (PLGA) NPs loaded with paclitaxel were conjugated with epithelial cell adhesion molecule (EpCAM) antibody for enhancing site-specific intracellular delivery of paclitaxel against EpCAM overexpressing RB cells. Methods PTX-loaded PLGA NPs were prepared by the oil-in-water single emulsion solvent evaporation method, and the PTX content in NPs was estimated by the reverse phase isocratic mode of high performance liquid chromatography. Ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride/N-hydroxysuccinimide chemistry was employed for the covalent attachment of monoclonal EpCAM antibody onto the NP surface. In vitro cytotoxicity of native PTX, unconjugated PTX-loaded NPs (PTX-NPs), and EpCAM antibody-conjugated PTX-loaded nanoparticles (PTX-NP-EpCAM) were evaluated on a Y79 RB cell line by a dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while cellular apoptosis, cysteinyl-aspartic acid protease (caspase)-3 activation, Poly (adenosine diphosphate-ribose) polymerase (PARP) cleavage, and cell-cycle arrest were quantified by flow cytometry. By employing flow cytometry and fluorescence image analyses, the extent of cellular uptake was comparatively evaluated. Results PTX-NP-EpCAM had superior antiproliferation activity, increased arrested cell population at the G2-M phase, and increased activation of caspase-3, followed by PARP cleavage in parallel with the induction of apoptosis. Increased uptake of PTX-Np-EpCAM by the cells suggests that they were mainly taken up through EpCAM mediated endocytosis. Conclusions EpCAM antibody-functionalized biodegradable NPs for tumor-selective drug delivery and overcoming drug resistance could be an efficient therapeutic strategy for retinoblastoma treatment. PMID:22065926

  2. II SEMINARIO INTERNACIONAL EN CIENCIAS APLICADAS “Una mirada hacia la innovación y el desarrollo sostenible”

    Directory of Open Access Journals (Sweden)

    Diana Verónica Cortés-Espinosa; et. al.

    2013-12-01

    Full Text Available INTRODUCCIÓN La Química, Biología y las Matemáticas son el pilar de las ciencias aplicadas. La investigación en el área básica se ve reflejada en el desarrollo de disciplinas como las ciencias de la salud, ingenierías y ciencias ambientales. De esta forma, la integración entre la investigación básica y las ciencias aplicadas es fundamental en el desarrollo del profesional moderno. Considerando lo anterior la Facultad de Ciencias Exactas Físicas y Naturales organiza el segundo seminario internacional en Ciencias Aplicadas,como una ventana a la integración de la investigación en la identificación de problemas cotidianos y a la búsqueda de soluciones a los mismos, contribuyendo a la vez el enriquecimiento profesional de los participantes y generando espacios de discusión científica en la Universidad de Santander UDES. OBJETIVOS Desarrollar temáticas de actualidad en diversas áreas del conocimiento con perspectivas de innovación y desarrollo tecnológico en áreas de la salud, investigación y el ambiente. Ofrecer talleres formativos para contextualizar el conocimiento de manera que los asistentes reconozcan las herramientas tecnológicas utilizadas para obtener mejores resultados en su futuro desempeño como profesionales.

  3. Investigación sobre el cotidiano del sujeto: oportunidades para una ciencia aplicada

    Directory of Open Access Journals (Sweden)

    Manuel Amezcua

    2012-01-01

    Full Text Available Desde un modelo de investigación emergente, la Investigación Aplicada a los Cuidados, se propone reflexionar sobre una de las áreas de indagación: la Investigación sobre el Cotidiano del Sujeto. El cotidiano del sujeto se conceptualiza como un espacio de interacción social donde las personas ejercen autonomía para tomar decisiones y ejecutar acciones para cuidar su salud. A través de diseños principalmente cualitativos, la Investigación sobre el Cotidiano del Sujeto aporta evidencias útiles y pertinentes para la práctica enfermera porque hablan del contexto y desde la perspectiva de los sujetos. Estas evidencias pueden encuadrarse en cuatro grandes dimensiones: la comprensión del padecimiento humano ante la enfermedad, la cultura del cuidado, la percepción sobre las intervenciones en salud, y el cotidiano del cuidador. Finalmente se plantean algunos desafíos que nos propone la Investigación sobre el Cotidiano del Sujeto e implican tres dimensiones imprescindibles en el ejercicio del cuidado: la dimensión ética, política y científica.

  4. Estados Unidos: do Iraque ao Brasil

    Directory of Open Access Journals (Sweden)

    Virgílio Caixeta Arraes

    2010-12-01

    Full Text Available O presente artigo busca analisar os recentes temas recorrentes na política externa norte-americana, caracterizada pela “guerra contra o terror”, e seus impactos para a agenda a ser discutida na vindoura visita do presidente dos Estados Unidos, Geoge W. Bush, ao Brasil.

  5. Critérios para a determinação da dose de nitrogênio a ser aplicada no tomateiro em ambiente protegido Criterions to rate nitrogen determination to be applied in the tomato plant in unheated greenhouse

    Directory of Open Access Journals (Sweden)

    Charles de Araujo

    2007-09-01

    Full Text Available Avaliou-se o efeito de critérios baseados na análise do teor de nitrato no solo, na produtividade esperada de frutos e em resultados de experimentos anteriores para a recomendação da dose de fertilizante nitrogenado a ser aplicada no tomateiro em ambiente protegido. Dois experimentos foram conduzidos em casa de vegetação, sendo um em solo previamente lixiviado com água (Experimento 1 e outro sem lixiviação (Experimento 2, a fim de simular condições de alto e baixo teor inicial de nitrato no solo. Seis tratamentos foram avaliados no delineamento de blocos ao acaso, com quatro repetições. Os tratamentos foram baseados nos critérios: 1 produtividade esperada de frutos e contribuições do solo e do fertilizante (PESF; 2 quantidade esperada de N no fruto e contribuição do solo (QECS; 3 quantidade esperada de N no fruto (QEFR; 4 dose recomendada experimentalmente de 280 kg ha-1 de N, aplicada parceladamente via gotejamento, em cobertura a cada 14 dias (DRCO; 5 mesma dose utilizada no tratamento anterior, mas aplicada em dose total no momento do transplante (DRTR; 6 não aplicação de fertilizante nitrogenado (TEST. As doses variaram de 0 a 570,8 kg ha-1 de N, dependendo do tratamento. Em ambos os experimentos as produções total e comercial de frutos não diferiram significativamente entre os tratamentos que receberam N. Maiores produções foram obtidas pela utilização do critério PESF. De forma geral, as eficiências de uso, de utilização e agronômica do N diminuíram à medida que a quantidade de N aplicada aumentou nos dois experimentos. Os critérios PESF e DRTR proporcionaram maior produtividade e eficiência na recuperação do N derivado do fertilizante.We evaluated the effect of criterions based on soil nitrate level, the expected fruit yield and previous experiments results to recommend nitrogen fertilizer rate to be applied in the tomato plants in unheated greenhouse. Two experiments were carried out, one in soil

  6. The Subaru Coronagraphic Extreme AO Project

    Science.gov (United States)

    Martinache, Frantz; Guyon, O.; Lozi, J.; Tamura, M.; Hodapp, K.; Suzuki, R.; Hayano, Y.; McElwain, M. W.

    2009-01-01

    While the existence of large numbers of extrasolar planets around solar type stars has been unambiguously demonstrated by radial velocity, transit and microlensing surveys, attempts at direct imaging with AO-equipped large telescopes remain unsuccessful. Because they supposedly offer more favorable contrast ratios, young systems consitute prime targets for imaging. Such observations will provide key insights on the formation and early evolution of planets and disks. Current surveys are limited by modest AO performance which limits inner working angle to 0.2", and only reach maximum sensitivity outside 1". This translates into orbital distances greater than 10 AU even on most nearby systems, while only 5 % of the known exoplanets have a semimajor axis greater than 10 AU. This calls for a major change of approach in the techniques used for direct imaging of the direct vicinity of stars. A sensible way to do the job is to combine coronagraphy and Extreme AO. Only accurate and fast control of the wavefront will permit the detection of high contrast planetary companions within 10 AU. The SCExAO system, currently under assembly, is an upgrade of the HiCIAO coronagraphic differential imaging camera, mounted behind the 188-actuator curvature AO system on Subaru Telescope. This platform includes a 1000-actuator MEMS deformable mirror for high accuracy wavefront correction and a PIAA coronagraph which delivers high contrast at 0.05" from the star (5 AU at 100 pc). Key technologies have been validated in the laboratory: high performance wavefront sensing schemes, spider vanes and central obstruction removal, and lossless beam apodization. The project is designed to be highly flexible to continuously integrate new technologies with high scientific payoff. Planned upgrades include an integral field unit for spectral characterization of planets/disks and a non-redundant aperture mask to push the performance of the system toward separations less than lambda/D.

  7. Matrine inhibits the growth of retinoblastoma cells (SO-Rb50) by decreasing proliferation and inducing apoptosis in a mitochondrial pathway.

    Science.gov (United States)

    Shao, Qingliang; Zhao, Xiaxia; Yao, Li

    2014-05-01

    Matrine, one of the main active components of extracts from the dry roots of Sophora flavescens, has potent anti-tumor activity in vitro and in vivo. Here, we investigated the apoptosis in matrine-treated retinoblastoma cells. The results showed that matrine could inhibit cell proliferation and induce apoptosis in a dose- and time-dependent manner. Further investigation revealed that a disruption of mitochondrial transmembrane potential and an up-regulation of reactive oxygen species in matrine-treated cells. By western blot analysis, we found that the up-regulation of cleaved Apaf-1, cleaved caspase-3, cleaved caspase-9, cleaved caspase-7, Bax/Bcl-2, varying with different concentration of matrine. These protein interactions may play a pivotal role in the regulation of apoptosis. Taken together, these results overall indicate that matrine could be used as an effective anti-tumor agent in therapy of retinoblastoma targets the caspase-dependent signaling pathway.

  8. Research progress in retinoblastoma gene therapy%基因治疗视网膜母细胞瘤的研究进展

    Institute of Scientific and Technical Information of China (English)

    郑敏明; 周希瑗

    2010-01-01

    基因治疗视网膜母细胞瘤(retinoblastoma,RB)是近年来新型的治疗方法.我们从自杀基因、抑癌基因、抗血管生成基因这三方面对RB的基因治疗现状、相关进展及存在问题作一综述.

  9. Regulation of corticotropin releasing hormone receptor type 1 messenger RNA level in Y-79 retinoblastoma cells: potential implications for human stress response and immune/inflammatory reaction

    OpenAIRE

    Vamvakopoulos, N C; Sioutopoulou, T. O.; Mamuris, Z.; Marcoulatos, P.; Avgerinos, P. C.

    1996-01-01

    We report the regulation of type 1 receptor mRNA in Y-79 human retinoblastoma cells, grown in the absence or presence of pharmacological levels of phorbol esters, forskolin, glucocorticoids and their combinations. To control for inducibility and for assessing the sensitivity of the Y-79 system to glucocorticoids, corticotropin releasing hormone mRNA levels were measured in parallel. All treatments stimulated corticotropin releasing hormone receptor type 1 gene expression relative to baseline....

  10. Individualized and combined modality therapy for retinoblastoma%视网膜母细胞瘤的个体化综合治疗研究进展

    Institute of Scientific and Technical Information of China (English)

    王亚峰

    2012-01-01

    Retinoblastoma(RB) is the most common intraocular malignant tumor in children. With the development of medical science and technology, the traditional therapy target and the mode of retinoblastoma are improving. Presently, the treatment methods of RB mainly include chemotherapy, external radiotherapy, operation, local therapy , biotherapy, etc. Treatment options of RB should be individualized to different patients, in order to achieve the best of therapeutic effect.%视网膜母细胞瘤( retinoblastoma,RB)是婴幼儿最常见的眼内恶性肿瘤.随着现代诊疗技术的提高,RB传统治疗目标和模式正在发生转变.目前RB的治疗方法主要包括化学减容法、外照射放疗、手术治疗、局部治疗、生物治疗等.RB治疗需根据不同的患者情况制定个性化的治疗方案,以达到最佳的治疗效果.

  11. SELETIVIDADE DA ASSOCIAÇÃO DOS HERBICIDAS IMAZAPIC E IMAZAPYR APLICADA EM DIFERENTES ESTÁDIOS DE DESENVOLVIMENTO DA CANA-DE-AÇÚCAR

    Directory of Open Access Journals (Sweden)

    OBARA, Flavio Eduardo Botelhos

    2015-05-01

    ao acaso no esquema de faixas, sendo que em uma direção de aplicação, as faixas foram constituídas de quatro doses da associação de imazapic + imazapyr, 0 + 0, 80,25 + 26,25, 120 + 40 e 133,75 + 43,75 g ha-1, e na outra direção a aplicação no mesmo dia dos herbicidas ametrina (2400 g ha-1, clomazone (1200 g ha-1, hexazinone (225 g ha-1, isoxaflutole (75 g ha-1, metribuzin (1440 g ha-1, tebuthiuron (750 g ha-1 e testemunha sem aplicação. Foram realizadas avaliações mensais e colheita das parcelas aos 150 DAT. A seletividade da associação de imazapic + imazapyr foi observada quando aplicada na dose de 80,25 + 26,25 g ha-1, na modalidade de pré-emergência, como também quando associada ao tebuthiuron, metribuzin e clomazone. Na aplicação em pós-emergência inicial a seletividade foi constatada pela aplicação de 80,25 + 26,25 g de imazapic + imazapyr ha-1, como pela associação desta dose com os herbicidas isoxaflutole e clomazone, dado que a aplicação em pós-emergência tardia se mostrou totalmente não seletiva para qualquer uso das associações dos herbicidas imazapic + imazapyr.

  12. Bioética aplicada à assistência de enfermagem

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    Valéria Lerch Lunardi

    1998-12-01

    Full Text Available A partir do conceito de governabilidade de Foucault como um tema ligado à ética, ao exercício da liberdade e da autonomia, discute o enfrentamento de situações de conflito ético, presentes no cotidiano da enfermagem, e em especial da enfermagem nefrológica. Defende a necessidade da enfermeira, na sua prática, optar por advogar a favor dos direitos do cliente.

  13. Labirintite consequente ao uso de difenilhidantoina

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    Eneida Baptistete Matarazzo

    1978-03-01

    Full Text Available É relatado o caso de paciente epilético que, aos 16 anos de idade, ao fazer uso de difenilhidantoina, apresentou, já nos primeiros dias, sinais de intolerância, com sensação de formigamento das extremidades. Aos 36 anos voltou a fazer uso desse mesmo medicamento e apresentou sintomas de labirintite, tendo crises de tipo rotatório quando inclinava a cabeça para a esquerda ou a movia bruscamente. O tratamento específico para labirintite, orientado por otorrinolaringologista, foi ineficaz e o quadro clínico agravou-se progressivamente. Ao fim de seis meses seu exame psíquico revelava leve torpor, lentificação do raciocínio, incapacidade para o trabalho e depressão reativa, observando-se, do ponto de vista neurológico, discreto desvio da marcha para a esquerda e discreto aumento da base de sustentação. Além disto referia a existência de crises rotatórias e vômitos tão frequentes que nos últimos dois meses precisou ser hospitalizado algumas vezes, para ser hidratado. A suspensão da difenilhidantoina com manutenção exclusiva de fenobarbital (do qual também já vinha fazendo uso, determinou regressão rápida dos sintomas citados. Tal fato permitiu concluir que a labirintite foi provocada pelo uso da difenilhidantoina e deve ser incluida na extensa relação de efeitos colaterais provocados por essa droga.

  14. Abordagens ao aprendizado político

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    Krischke, Paulo J.

    2002-01-01

    Full Text Available Há um reconhecimento crescente entre os cientistas políticos de que uma fonte principal para a conquista e o aperfeiçoamento da democracia é o aprendizado político por parte dos atores sociais e políticos. Contudo, a bibliografia especializada não considera satisfatoriamente como o aprendizado político reorienta os comportamentos e atitudes em apoio à democracia política e seu aperfeiçoamento. A presente proposta acata esse desafio, numa revisão teórica inicial das abordagens existentes ao aprendizado político no contexto da globalização. Num primeiro momento, considera as limitações da abordagem ao estudo das elites nos processos de democratização. Em segundo lugar salienta as dificuldades da teoria da modernização para enfrentar o tema, nos estudos sobre os públicos massivos. Mais adiante sugere as contribuições que uma abordagem inspirada nos trabalhos de Jürgen Habermas pode proporcionar ao estudo do aprendizado político. E conclui indicando certas interfaces temáticas a serem examinadas na bibliografia no contexto da globalização. À luz de resultados de uma pesquisa atualmente em sua fase final

  15. 视网膜母细胞瘤的非典型临床表现分析%Unusual presentation of retinoblastoma

    Institute of Scientific and Technical Information of China (English)

    朱丽娟; 颜建华; 鲁志卿; 高韶晖

    2009-01-01

    目的 分析视网膜母细胞瘤患者的非典型临床表现.以提高临床诊治水平.方法 对1995~2004年期间,中山大学中山眼科中心住院治疗的350例视网膜母细胞瘤患者的临床资料进行回顾性分析.结果 350例视网膜母细胞瘤患者中表现为白瞳者298例(85.1%),斜视29例(8.3%).非典型临床表现有:眼球震颤1例(0.3%),眼睑肿胀1例(013%),角膜水肿36例(10.3%),前房积血13(3.7%),"前房积脓"16例(4.6%),虹膜红变10例(2.9%),虹膜结节1例(0.3%).晶体混浊3例(0.9%),玻璃体积血1例(0.3%),视网膜脱离9例(2.6%),眼球痨5例(1.4%).结论 视网膜母细胞瘤具有多种临床表现,熟知其非典型临床表现可减少误诊漏诊,利于早期治疗,提高患者预后.%Objective To study the atypical presenting signs of retinoblastoma in our institution. Methods A retrospective study was performed on the records of 350 patients treated for retinoblastoma at Zhongshan Ophthalmic Center between 1994 and 2005.Results A total records of 350 cases of retinoblas-toma were reviewed.85.1% of the patients had leukocoria, 8.3% of the patients had strabismus.Uncommon presenting signs include: nystagmus (0.3%), eyelid swelling (0.3%), corneal edema (10.3%), hyphema (3.7%), pseudohypopyon (4.6%), iris neovascularization (2.9%),iris nodules (0.3%), cataract (0.3%),vilreous hemorrhage (0.3%),retina detachment (2.6%),phthisis bulbi (1.4%).Conclusions Varied presentations of retinoblastoma, especially atypical presentation should alert the clinician to suspect retinoblastoma.

  16. Resistência de biótipos de arroz-vermelho aos herbicidas imazapyr + imazapic e alternativas de controle

    Directory of Open Access Journals (Sweden)

    Rafael Salles Rubin

    2014-10-01

    Full Text Available O uso intenso de herbicidas com o mesmo mecanismo de ação na cultura do arroz (Oryza sativa L. tem selecionado biótipos resistentes, como, por exemplo, o arroz-vermelho (planta daninha resistente aos herbicidas imidazolinonas. Por essa razão, este trabalho foi desenvolvido com o objetivo de avaliar a resistência de arroz-vermelho ao herbicida imazapyr + imazapic, na região sul do Rio Grande do Sul, e o controle do biótipo resistente de arroz-vermelho com os herbicidas alternativos clethodim e glyphosate. Foram realizados três experimentos, em delineamento casualizado, arranjados em esquema fatorial. No primeiro experimento, o fator A testou biótipos de arroz-vermelho [ORYSA 184 (resistente e ORYSA 188 (susceptível], o fator B comparou herbicidas (imazapyr + imazapic, clethodim e glyphosate e o fator C avaliou doses dos herbicidas (0; 0,5; 1; 2; 4; 8; 16 e 32 vezes a dose recomendada. No segundo, compararam-se biótipos de arroz-vermelho e doses do herbicida imazapyr + imazapic (0; 0,5; 1; 2; 4; 8; 16; 32 e 64 vezes a dose recomendada. No terceiro, testaram-se biótipos de arroz-vermelho e doses diferentes do herbicida imazapyr + imazapic para cada biótipo. O biótipo ORYSA 184 é resistente ao imazapyr + imazapic, quando aplicada a dose máxima de registro e estádio indicado. Os herbicidas clethodim e glyphosate, detentores de mecanismos de ação alternativos, controlam o biótipo resistente ORYSA 184 de arroz-vermelho.

  17. Análise macroscópica e histológica do emprego da poliuretana derivada do óleo de mamona (Ricinus communis aplicada na tíbia de cães em fase de crescimento Macroscopic and histopathologic evaluations of the use of polyurethane resins derived from castor oil (Ricinus communis applied in the tibia of the puppies

    Directory of Open Access Journals (Sweden)

    Patricia Popak Maria

    2003-08-01

    Full Text Available OBJETIVO: Estudar o efeito da poliuretana de mamona aplicada ao osso de cães em crescimento. MÉTODOS: Foram utilizados 12 cães subdivididos aleatoriamente em 3 grupos, os quais receberam o implante de mamona na face medial proximal da tíbia, com análise macroscópica e histopatológica aos 30 (GIII, 60 (GII e 90 (GI dias. RESULTADOS: A poliuretana foi recoberta por uma cápsula conjuntiva fibrosa, não ocorrendo proliferação óssea ao seu redor. CONCLUSÃO: A poliuretana é biocompatível, comportando-se como espaçador biológico em cães. Nesta espécie não ocorre a osteointegração.PURPOSE: To analyze the effects of castor oil derived poliurethane, when applied to bones of puppies. METHODS: Twelve dogs, randomly divided in 3 groups, received a castor oil derived poliurethane at the medial side of the proximal tibia. Gross and histopathologic evaluations were performed at 30 (GIII, 60 (GII and 90 (GI days. RESULTS: The poliurethane was enclosed in a connective fibrous tissue capsule and there was no new bone formation around it. CONCLUSION: The castor oil derived poliurethane is biocompatible and behaves as a biological "stand off" in dogs. Osteointegration does not occur in this species.

  18. Associação entre polimorfismo SLC6A3 3’UTR VNTR e a resposta ao tratamento da dependência de nicotina

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    Guilherme Rubino de Azevedo Focchi

    2011-01-01

    Full Text Available Objetivo: Avaliar a associação entre a resposta ao tratamento da dependência de nicotina com bupropiona e a presença do polimorfismo SLC6A3 3’UTR VNTR, localizado no gene que codifica o transportador dopaminérgico. Método: Foram acompanhados no Ambulatório de Tabagismo do Instituto de Psiquiatria da Faculdade de Medicina da USP 100 pacientes do sexo masculino com diagnóstico de dependência de nicotina, sem outras patologias. Todos receberam bupropiona até 300 mg ao dia por 12 semanas, associada à terapia cognitivo-comportamental em grupo. A Escala de Fagerström foi aplicada no início e no final do tratamento, e avaliou-se a parada do uso de cigarros na última semana de tratamento e um mês após. Os pacientes tiveram 10 ml de sangue colhidos e genotipados para a existência do polimorfismo SLC6A3 3’UTR VNTR. Resultados: Não foi encontrada associação entre cessação do uso de cigarro e presença do polimorfismo. Conclusão: São necessários mais estudos para avaliar se a presença do polimorfismo SLC6A3 3’UTR VNTR estaria relacionada à melhor resposta ao tratamento da dependência de nicotina.

  19. Some polemical issues in Applied Linguistics Alguns temas polêmicos na disciplina de linguística aplicada

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    John Robert Schmitz

    2010-01-01

    Full Text Available In this paper, I look at three polemical issues in Applied Linguistics. I argue, first of all, that the desire for a stable definition of applied linguistics has by no means prevented research in the discipline. Secondly, I contend that the notion or "tradition" of "linguistics applied" (corpus linguistics or lexicography is broader and more serious than "applicationism" (the use of linguistic formalisms, artificial practices, and terminology in teaching material that are problematic and motivated by commercial interests. Thirdly, I argue that Educational Linguistics and Applied Linguistics have overlapping research objectives. In the course of the paper, I present some reservations about Educational Linguistics.Neste trabalho, examino três temas polêmicos na disciplina de Linguística Aplicada. Argumento, em primeiro lugar, que o desejo de uma definição estável de Linguística Aplicada, de nenhuma forma, tem impedido a pesquisa no âmbito da disciplina. Em segundo lugar, argumento que a "Linguistics Applied" (a linguística do corpus ou a lexicologia são muito mais abrangentes e sérias do que as práticas "aplicacionistas" (o uso de formalismo, artificialismos e nomenclatura em material didático que são problemáticos e motivados por interesses comerciais. Em terceiro lugar, argumento que a Linguística Educacional e a Linguística Aplicada têm objetivos de pesquisa que se sobrepõem. No decorrer do trabalho, apresento algumas reservas minhas sobre a Linguística Educacional.

  20. Inteligência artificial aplicada à Zootecnia Artificial intelligence in Animal Science

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    Ernane José Xavier Costa

    2009-07-01

    Full Text Available Os sistemas biológicos são surpreendentemente flexíveis pra processar informação proveniente do mundo real. Alguns organismos biológicos possuem uma unidade central de processamento denominada de cérebro. O cérebro humano consiste de 10(11 neurônios e realiza processamento inteligente de forma exata e subjetiva. A Inteligência Artificial (IA tenta trazer para o mundo da computação digital a heurística dos sistemas biológicos de várias maneiras, mas, ainda resta muito para que isso seja concretizado. No entanto, algumas técnicas como Redes neurais artificiais e lógica fuzzy tem mostrado efetivas para resolver problemas complexos usando a heurística dos sistemas biológicos. Recentemente o numero de aplicação dos métodos da IA em sistemas zootécnicos tem aumentado significativamente. O objetivo deste artigo é explicar os princípios básicos da resolução de problemas usando heurística e demonstrar como a IA pode ser aplicada para construir um sistema especialista para resolver problemas na área de zootecnia.Biological systems are surprising flexible in processing information in the real world. Some biological organisms have a central unit processing named brain. The human's brain, consisting of 10(11 neurons, realizes intelligent information processing based on exact and commonsense reasoning. Artificial intelligence (AI has been trying to implement biological intelligence in computers in various ways, but is still far from real one. Therefore, there are approaches like Symbolic AI, Artificial Neural Network and Fuzzy system that partially successful in implementing heuristic from biological intelligence. Many recent applications of these approaches show an increased interest in animal science research. The main goal of this article is to explain the principles of heuristic problem-solving approach and to demonstrate how they can be applied to building knowledge-based systems for animal science problem solving.

  1. Medidas de frecuencia, asociación e impacto en investigación aplicada

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    J.A. Mirón Canelo

    2008-06-01

    Full Text Available En la Sociedad actual del conocimiento y la información es preciso disponer de herramientas básicas para medir los fenómenos epidemiológicos como la enfermedad, la incapacidad o la siniestralidad laboral. Las formas de medir son instrumentos que se deben conocer y aplicar para planificar y tomar decisiones en Salud Pública y en Salud Laboral. El objetivo de este artículo es dar a conocer las principales medidas de frecuencia, asociación e impacto utilizadas en investigación aplicada, clínica o de Salud Pública para tratar de medir, valorar y estimar la importancia de los problemas de salud y enfermedades de relacionadas con el trabajo, de los factores de riesgo ocupacionales y otros eventos relacionados con la seguridad e higiene en el trabajo y, en definitiva, con la Salud de los trabajadores. Se describen los conceptos teóricos de las formas de medir en epidemiología, su interpretación y aplicación práctica de los indicadores básicos utilizados en la práctica habitual de los profesionales de las Ciencias de la Salud. Su utilidad fundamental es poder disponer de una información objetiva, fiable y precisa que permita tomar decisiones adecuadas y pertinentes en relación con la prevención, seguridad laboral, atención y rehabilitación de los trabajadores.In the society´current knowledge and information is necessary to have basic tools to measure the epidemic phenomena such as illness, disability or workplace accidents. The ways of measuring are instruments that they must know and apply to plan and take decisions on Public Health and Labour/Occupational Health. The aim of this article is to inform about the most important measures of frequency, association and impact used in applied research, clinical or Public Health to try to measure, to value and estimate the importance of health problems and diseases related work. The occupational risk factors and other events related to safety and healthy working conditions and ultimately

  2. Minería de datos aplicada en detección de intrusos

    Directory of Open Access Journals (Sweden)

    Diego Vallejo P.

    2012-06-01

    Full Text Available Con base a los fundamentos y técnicas de la minería de datos se pueden diseñar y elaborar modelos que permiten encontrar comportamientos clandestinos de fácil detección a simple vista como lo es la información no evidente -desconocida a priori y potencialmente útil- en referencia a hechos determinados. En particular la utilidad de la minería de datos en esta área radica en una serie de técnicas, algoritmos y métodos que imitan la característica humana del aprendizaje: ser capaz de extraer nuevos conocimientos a partir de las experiencias. La minería de datos posee características como: análisis de grandes volúmenes de información, generación de comportamientos que no son fácilmente perceptibles, depuración de datos para toma de decisiones. Estas características pueden ser de vital importancia para ser aplicadas en la seguridad de la información a través de la detección de intrusos. En la actualidad la seguridad de la información es uno de los grandes retos que tiene el mundo, y en especial, la detección de anomalías en los registros de acceso de los diferentes sistemas de información. Con esta aplicabilidad resulta un método básico y muy eficiente de poder prevenir intrusiones. Se centra el campo de en la detección de intrusos al nutrir el proceso de seguimiento de los acontecimientos que ocurren en la red informática, seguido del análisis de los mismos; con el fin de detectar los factores que ponen en peligro la confidencialidad, integridad, disponibilidad y no repudio de los datos. En el presente trabajo se pretende mostrar el aporte a la seguridad de la información de la minería de datos en el contexto de la detección de intrusos.

  3. TEORIA DO PROSPECTO: EVIDÊNCIAS APLICADAS EM FINANÇAS COMPORTAMENTAIS

    Directory of Open Access Journals (Sweden)

    Thiago de Sousa Barros

    2015-11-01

    Full Text Available As Finanças Comportamentais tem sido uma via alternativa para a compreensão da realidade econômica e financeira através de um viés mais abrangente, contrapondo alguns paradigmas e preceitos das Finanças Tradicionais e seus respectivos modelos. Em face disso, este artigo analisa elementos fundamentais das finanças comportamentais e, para este efeito, utiliza a Teoria do Prospecto, pesquisa empírica desenvolvida por Kahneman e Tversky (1979, para investigar a manifestação dos efeitos certeza, reflexo e isolamento no processo de decisão por parte dos agentes e a influência do gênero nas questões relacionadas às perdas e exposição ao risco. Assim, através de uma pesquisa de campo, foram aplicados questionários numa amostra de 150 discentes do curso de Ciências Econômicas da Universidade Federal de Ouro Preto e os dados foram tratados através de estatística descritiva e um teste não paramétrico (Qui-Quadrado. Os resultados confirmam empiricamente a existência dos três efeitos preconizados na Teoria do Prospecto e as influências de vieses cognitivos ao longo do processo decisório por parte dos agentes, além de evidenciar diferenças importantes quando da separação por gêneros, já que as mulheres, em determinadas ocasiões de ganho, mostraram-se mais avessas ao risco que os homens.

  4. Sociologia aplicada: a experiência dos intelectuais do Dieese e do Cesit

    OpenAIRE

    2007-01-01

    A principal proposta deste estudo é apontar alguns produtos da .escola uspiana de sociologia. durante a década de 1960. Neste estudo buscaremos pesquisar a incursão do intelectual no tecido social e a aplicação da sociologia a partir da exposição das realizações de dois grupos de intelectuais alocados em instituições diferenciadas: o Departamento Intersindical de Estatística e Estudos Sócio-Econômicos (DIEESE/1955), vinculado ao meio sindical, e o Centro de Sociologia Industrial e do Trabalho...

  5. Optimização estrutural aplicada à melhoria da precisão de quinagem

    OpenAIRE

    2012-01-01

    Dissertação para obtenção do Grau de Mestre em Engenharia Mecânica A presente Dissertação tem como objectivo a obtenção das dimensões dos aventais de uma quinadora que permitam minimizar o Erro de precisão de quinagem associado ao processo de quinagem chapa. Começa-se por identificar que o Erro está associado à ausência de paralelismo entre os aventais da quinadora aquando do carregamento destes sobre a chapa. A estrutura dos aventais pode ser aproximada por vigas de secção ...

  6. Interaction of the retinoblastoma protein with Orc1 and its recruitment to human origins of DNA replication.

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    Ramiro Mendoza-Maldonado

    Full Text Available BACKGROUND: The retinoblastoma protein (Rb is a crucial regulator of cell cycle progression by binding with E2F transcription factor and repressing the expression of a variety of genes required for the G1-S phase transition. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that Rb and E2F1 directly participate in the control of initiation of DNA replication in human HeLa, U2OS and T98G cells by specifically binding to origins of DNA replication in a cell cycle regulated manner. We show that, both in vitro and inside the cells, the largest subunit of the origin recognition complex (Orc1 specifically binds hypo-phosphorylated Rb and that this interaction is competitive with the binding of Rb to E2F1. The displacement of Rb-bound Orc1 by E2F1 at origins of DNA replication marks the progression of the G1 phase of the cell cycle toward the G1-S border. CONCLUSIONS/SIGNIFICANCE: The participation of Rb and E2F1 in the formation of the multiprotein complex that binds origins of DNA replication in mammalian cells appears to represent an effective mechanism to couple the expression of genes required for cell cycle progression to the activation of DNA replication.

  7. Docosahexaenoic acid inhibits cancer cell growth via p27Kip1, CDK2, ERK1/ERK2, and retinoblastoma phosphorylation.

    Science.gov (United States)

    Khan, Naim A; Nishimura, Kazuhiro; Aires, Virginie; Yamashita, Tomoko; Oaxaca-Castillo, David; Kashiwagi, Keiko; Igarashi, Kazuei

    2006-10-01

    Docosahexaenoic acid (DHA), a PUFA of the n-3 family, inhibited the growth of FM3A mouse mammary cancer cells by arresting their progression from the late-G(1) to the S phase of the cell cycle. DHA upregulated p27(Kip1) levels by inhibiting phosphorylation of mitogen-activated protein (MAP) kinases, i.e., ERK1/ERK2. Indeed, inhibition of ERK1/ERK2 phosphorylation by DHA, U0126 [chemical MAPK extracellularly signal-regulated kinase kinase (MEK) inhibitor], and MEK(SA) (cells expressing dominant negative constructs of MEK) resulted in the accumulation of p27(Kip1). MAP kinase (MAPK) inhibition by DHA did not increase p27(Kip1) mRNA levels. Rather, this fatty acid stabilized p27(Kip1) contents and inhibited MAPK-dependent proteasomal degradation of this protein. DHA also diminished cyclin E phosphorylation, cyclin-dependent kinase-2 (CDK2) activity, and phosphorylation of retinoblastoma protein in these cells. Our study shows that DHA arrests cell growth by modulating the phosphorylation of cell cycle-related proteins.

  8. The retinoblastoma protein regulates hypoxia-inducible genetic programs, tumor cell invasiveness and neuroendocrine differentiation in prostate cancer cells

    Science.gov (United States)

    Labrecque, Mark P.; Takhar, Mandeep K.; Nason, Rebecca; Santacruz, Stephanie; Tam, Kevin J.; Massah, Shabnam; Haegert, Anne; Bell, Robert H.; Altamirano-Dimas, Manuel; Collins, Colin C.; Lee, Frank J.S.; Prefontaine, Gratien G.; Cox, Michael E.; Beischlag, Timothy V.

    2016-01-01

    Loss of tumor suppressor proteins, such as the retinoblastoma protein (Rb), results in tumor progression and metastasis. Metastasis is facilitated by low oxygen availability within the tumor that is detected by hypoxia inducible factors (HIFs). The HIF1 complex, HIF1α and dimerization partner the aryl hydrocarbon receptor nuclear translocator (ARNT), is the master regulator of the hypoxic response. Previously, we demonstrated that Rb represses the transcriptional response to hypoxia by virtue of its association with HIF1. In this report, we further characterized the role Rb plays in mediating hypoxia-regulated genetic programs by stably ablating Rb expression with retrovirally-introduced short hairpin RNA in LNCaP and 22Rv1 human prostate cancer cells. DNA microarray analysis revealed that loss of Rb in conjunction with hypoxia leads to aberrant expression of hypoxia-regulated genetic programs that increase cell invasion and promote neuroendocrine differentiation. For the first time, we have established a direct link between hypoxic tumor environments, Rb inactivation and progression to late stage metastatic neuroendocrine prostate cancer. Understanding the molecular pathways responsible for progression of benign prostate tumors to metastasized and lethal forms will aid in the development of more effective prostate cancer therapies. PMID:27015368

  9. Identification of alpha 2-adrenergic receptor sites in human retinoblastoma (Y-79) and neuroblastoma (SH-SY5Y) cells

    Energy Technology Data Exchange (ETDEWEB)

    Kazmi, S.M.; Mishra, R.K.

    1989-02-15

    The existence of specific alpha 2-adrenergic receptor sites has been shown in human retinoblastoma (Y-79) and neuroblastoma (SH-SH5Y) cells using direct radioligand binding. (/sup 3/H)Rauwolscine, a selective alpha 2-adrenergic receptor antagonist, exhibited high affinity, saturable binding to both Y-79 and SH-SY5Y cell membranes. The binding of alpha 1 specific antagonist, (/sup 3/H)Prazocine, was not detectable in either cell type. Competition studies with antagonists yielded pharmacological characteristics typical of alpha 2-adrenergic receptors: rauwolscine greater than yohimbine greater than phentolamine greater than prazocine. Based on the affinity constants of prazocine and oxymetazoline, it appears that Y-79 cells contain alpha 2A receptor, whereas SH-SY5Y cells probably represent a mixture of alpha 2A and alpha 2B receptors. alpha 2-agonists clonidine and (-)epinephrine inhibition curves yielded high and low affinity states of the receptor in SH-SY5Y cells. Gpp(NH)p and sodium ions reduced the proportion of high affinity sites of alpha 2 receptors. These two neuronal cell lines of human origin would prove useful in elucidating the action and regulation of human alpha 2-adrenergic receptors and their interaction with other receptor systems.

  10. Histone demethylase retinoblastoma binding protein 2 regulates the expression of α-smooth muscle actin and vimentin in cirrhotic livers

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Q. [Department of Microbiology, Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, School of Medicine, Shandong University, Jinan (China); Wang, L.X. [Department of Pharmacology, School of Medicine, Shandong University, Jinan (China); Zeng, J.P. [Department of Biochemistry, School of Medicine, Shandong University, Jinan (China); Liu, X.J.; Liang, X.M.; Zhou, Y.B. [Department of Microbiology, Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, School of Medicine, Shandong University, Jinan (China)

    2013-09-06

    Liver cirrhosis is one of the most common diseases of Chinese patients. Herein, we report the high expression of a newly identified histone 3 lysine 4 demethylase, retinoblastoma binding protein 2 (RBP2), and its role in liver cirrhosis in humans. The siRNA knockdown of RBP2 expression in hepatic stellate cells (HSCs) reduced levels of α-smooth muscle actin (α-SMA) and vimentin and decreased the proliferation of HSCs; and overexpression of RBP2 increased α-SMA and vimentin levels. Treatment with transforming growth factor β (TGF-β) upregulated the expression of RBP2, α-SMA, and vimentin, and the siRNA knockdown of RBP2 expression attenuated TGF-β-mediated upregulation of α-SMA and vimentin expression and HSC proliferation. Furthermore, RBP2 was highly expressed in cirrhotic rat livers. Therefore, RBP2 may participate in the pathogenesis of liver cirrhosis by regulating the expression of α-SMA and vimentin. RBP2 may be a useful marker for the diagnosis and treatment of liver cirrhosis.

  11. The retinoblastoma protein regulates hypoxia-inducible genetic programs, tumor cell invasiveness and neuroendocrine differentiation in prostate cancer cells.

    Science.gov (United States)

    Labrecque, Mark P; Takhar, Mandeep K; Nason, Rebecca; Santacruz, Stephanie; Tam, Kevin J; Massah, Shabnam; Haegert, Anne; Bell, Robert H; Altamirano-Dimas, Manuel; Collins, Colin C; Lee, Frank J S; Prefontaine, Gratien G; Cox, Michael E; Beischlag, Timothy V

    2016-04-26

    Loss of tumor suppressor proteins, such as the retinoblastoma protein (Rb), results in tumor progression and metastasis. Metastasis is facilitated by low oxygen availability within the tumor that is detected by hypoxia inducible factors (HIFs). The HIF1 complex, HIF1α and dimerization partner the aryl hydrocarbon receptor nuclear translocator (ARNT), is the master regulator of the hypoxic response. Previously, we demonstrated that Rb represses the transcriptional response to hypoxia by virtue of its association with HIF1. In this report, we further characterized the role Rb plays in mediating hypoxia-regulated genetic programs by stably ablating Rb expression with retrovirally-introduced short hairpin RNA in LNCaP and 22Rv1 human prostate cancer cells. DNA microarray analysis revealed that loss of Rb in conjunction with hypoxia leads to aberrant expression of hypoxia-regulated genetic programs that increase cell invasion and promote neuroendocrine differentiation. For the first time, we have established a direct link between hypoxic tumor environments, Rb inactivation and progression to late stage metastatic neuroendocrine prostate cancer. Understanding the molecular pathways responsible for progression of benign prostate tumors to metastasized and lethal forms will aid in the development of more effective prostate cancer therapies.

  12. Blocking the maturation of OncomiRNAs using pri-miRNA-17∼92 aptamer in retinoblastoma.

    Science.gov (United States)

    Subramanian, Nithya; Kanwar, Jagat R; Kanwar, Rupinder K; Krishnakumar, Subramanian

    2015-02-01

    The miR-17∼92. or oncomiR-1, cluster encodes oncogenic microRNAs (miRNAs), and it also promotes retinoblastoma (RB) tumor formation. Antagomir and miRNA mimics based approaches are widely tried against oncogenic and tumor suppressive miRNAs. Other methods for targeting cancer related miRNAs are still under development. In the current study, we focused on the pri-miRNA-17∼92 aptamer (pri-apt), which can potentially replace the mix of five antagomirs by one aptamer that function to abrogate the maturation of miR-17, miR-18a, and miR-19b (Ppri-apt led to S-phase arrest in WERI-Rb1 cells and onset of apoptosis in both Y79 and WERI-Rb1 cell lines. There was increased cytotoxicity as measured by lactate dehydrogenase activity in pri-apt treated Y79 cells (Ppri-apt in RB cell lines, which can be readily modified by developing appropriate vectors for the delivery of the aptamer specifically to cancer cells.

  13. Role of the retinoblastoma protein in cell cycle arrest mediated by a novel cell surface proliferation inhibitor

    Science.gov (United States)

    Enebo, D. J.; Fattaey, H. K.; Moos, P. J.; Johnson, T. C.; Spooner, B. S. (Principal Investigator)

    1994-01-01

    A novel cell regulatory sialoglycopeptide (CeReS-18), purified from the cell surface of bovine cerebral cortex cells has been shown to be a potent and reversible inhibitor of proliferation of a wide array of fibroblasts as well as epithelial-like cells and nontransformed and transformed cells. To investigate the possible mechanisms by which CeReS-18 exerts its inhibitory action, the effect of the inhibitor on the posttranslational regulation of the retinoblastoma susceptibility gene product (RB), a tumor suppressor gene, has been examined. It is shown that CeReS-18 mediated cell cycle arrest of both human diploid fibroblasts (HSBP) and mouse fibroblasts (Swiss 3T3) results in the maintenance of the RB protein in the hypophosphorylated state, consistent with a late G1 arrest site. Although their normal nontransformed counterparts are sensitive to cell cycle arrest mediated by CeReS-18, cell lines lacking a functional RB protein, through either genetic mutation or DNA tumor virus oncoprotein interaction, are less sensitive. The refractory nature of these cells is shown to be independent of specific surface receptors for the inhibitor, and another tumor suppressor gene (p53) does not appear to be involved in the CeReS-18 inhibition of cell proliferation. The requirement for a functional RB protein product, in order for CeReS-18 to mediate cell cycle arrest, is discussed in light of regulatory events associated with density-dependent growth inhibition.

  14. Structure-function analysis of the retinoblastoma tumor suppressor protein – is the whole a sum of its parts?

    Directory of Open Access Journals (Sweden)

    Dick Frederick A

    2007-09-01

    Full Text Available Abstract Biochemical analysis of the retinoblastoma protein's function has received considerable attention since it was cloned just over 20 years ago. During this time pRB has emerged as a key regulator of the cell division cycle and its ability to block proliferation is disrupted in the vast majority of human cancers. Much has been learned about the regulation of E2F transcription factors by pRB in the cell cycle. However, many questions remain unresolved and researchers continue to explore this multifunctional protein. In particular, understanding how its biochemical functions contribute to its role as a tumor suppressor remains to be determined. Since pRB has been shown to function as an adaptor molecule that links different proteins together, or to particular promoters, analyzing pRB by disrupting individual protein interactions holds tremendous promise in unraveling the intricacies of its function. Recently, crystal structures have reported how pRB interacts with some of its molecular partners. This information has created the possibility of rationally separating pRB functions by studying mutants that disrupt individual binding sites. This review will focus on literature that investigates pRB by isolating functions based on binding sites within the pocket domain. This article will also discuss the prospects for using this approach to further explore the unknown functions of pRB.

  15. Esta é minha carta ao mundo

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    Fernanda Mourão

    2009-01-01

    Full Text Available A partir da primeira carta de Emily Dickinson (1830-1886 a Thomas Higginson, que então seria seu “preceptor” e interlocutor para sempre, este texto propõe uma leitura da obra da escritora norte-americana a partir do biografema da carta e da ideia de sua obra como “carta ao mundo” – conforme um de seus mais famosos poemas –, com todas as implicações trazidas pelo termo, e à luz das teorias de Roland Barthes, Maurice Blanchot e Silvina Rodrigues Lopes, entre outros.

  16. AO corrected satellite imaging from Mount Stromlo

    Science.gov (United States)

    Bennet, F.; Rigaut, F.; Price, I.; Herrald, N.; Ritchie, I.; Smith, C.

    2016-07-01

    The Research School of Astronomy and Astrophysics have been developing adaptive optics systems for space situational awareness. As part of this program we have developed satellite imaging using compact adaptive optics systems for small (1-2 m) telescopes such as those operated by Electro Optic Systems (EOS) from the Mount Stromlo Observatory. We have focused on making compact, simple, and high performance AO systems using modern high stroke high speed deformable mirrors and EMCCD cameras. We are able to track satellites down to magnitude 10 with a Strehl in excess of 20% in median seeing.

  17. Ciencia básica y ciencia aplicada Basic science and applied science

    Directory of Open Access Journals (Sweden)

    Ruy Pérez-Tamayo

    2001-08-01

    Full Text Available En el contexto de una intervención en el Foro de Consulta Democrática de la Coordinación de los Institutos Nacionales de Salud sobre Enseñanza e Investigación en Salud, realizado en el Instituto Nacional de Cardiología Ignacio Chávez, se hace una revisión crítica de la clasificación convencional de la ciencia en básica y aplicada; además, se analiza lo que deberían ser la enseñanza y la investigación en salud. Para apoyar su propia clasificación de la ciencia en "bien hecha", la cual "genera conocimientos verificables sobre la realidad" y la "mal hecha", improductiva o productora de "puras mentiras" y "no debe patrocinarse", el autor revisa las definiciones utilitaristas y peyorativas como las que establecen ciencia comprometida y ciencia pura, ciencia útil y ciencia inútil, y ciencia práctica y ciencia esotérica, como sinónimos de ciencia aplicada y ciencia básica y afirma que, en México, esta diferenciación "para lo único que ha servido en el pasado es para justificar la reducción en el apoyo oficial a la ciencia básica, porque no estaba dirigida a resolver ´los problemas nacionales´o porque no caía en las prioridades establecidas en ese sexenio". En cuanto a educación e investigación en salud reconoce que el programa actual de formación de investigadores tiene una eficiencia muy baja y propone un estudio científico crítico, realizado por un equipo de especialistas, interdisciplinario, "para integrar la carrera del investigador científico desde la captura de la juventud inteligente hasta la jubilación o muerte del investigador" y para el cual la evaluación de la eficiencia del apoyo a sus proyectos de investigación no se restrinja a si se publicó o no un artículo "pues la calidad del trabajo científico y la contribución de un investigador al desarrollo de la ciencia no es nada más el número de sus publicaciones". El texto completo en inglés de este artículo está disponible en: http

  18. Creación del Instituto Universitario de Física Aplicada a las Ciencias y las Tecnologías (IUFACyT)

    OpenAIRE

    Beléndez Vázquez, Augusto

    2010-01-01

    El 31 de julio de 2009 el Consell de la Generalitat Valenciana aprobó la creación del Instituto Universitario de Física Aplicada a las Ciencias y las Tecnologías (IUFACyT) en la Universidad de Alicante, cuyo decreto de creación fue publicado en el DOCV el 4 de agosto de 2009. El objetivo primordial de este Instituto es fomentar la investigación básica - clave para el resto de procesos científicos - y aplicada en el campo de la Física, fomentando aplicaciones y aspectos de interés de la misma ...

  19. Metodologia de Sistemas Flexíveis aplicada a estudos em Ciência da Informação: uma experiência pedagógica

    Directory of Open Access Journals (Sweden)

    Sely M.S. Costa

    Full Text Available Este trabalho descreve a aplicação da Metodologia de Sistemas Flexíveis - Soft Systems Methodology a um programa de mudança organizacional no Departamento de Ciência da Informação da Universidade de Brasília. A metodologia em questão foi desenvolvida por Peter Checkland, como alternativa às metodologias de sistemas rígidos, como a pesquisa operacional. Tem sido aplicada nas mais diversificadas situações, entre outras, para projetos relacionados com situações-problema que ocorrem em organizações. Mais recentemente, aplicou-se com freqüência em pesquisas acadêmicas na área de Ciência da Informação, especialmente na Inglaterra, e agora, no Brasil, onde o Soft Systems Methodology foi introduzido como disciplina específica do programa de pós-graduação do Departamento de Ciência da Informação da Universidade de Brasília e da orientação de teses e dissertações. Este trabalho discute a Soft Systems Methodology e seus fundamentos, assim como a experiência de seu ensino na pós-graduação e sua aplicação em problemas da Ciência da Informação. A Soft Systems Methodology consiste de sete estágios e faz uso de ferramentas específicas, sendo fundamentada na abordagem sistêmica, na fenomenologia e na Pesquisa-Ação. Este trabalho descreve ainda, a partir do levantamento bibliográfico sobre o Soft Systems Methodology, a organização do conteúdo programático da disciplina, atendendo a alunos de mestrado e doutorado em Ciência da Informação, e a observação sistemática, tanto das percepções dos alunos e suas reações relativas à disciplina durante todo o período letivo, quanto da aplicação da Soft Systems Methodology em situações-problema reais. Incluem-se na discussão as ações desenvolvidas visando melhor interação entre alunos de graduação, bolsistas do PIBIC, e alunos de pós-graduação do citado departamento, cujo perfil educacional e profissional era variado: Engenharia, Ciência da

  20. Nefrocalcinose associada ao uso de esteroide anabolizante

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    Weverton Machado Luchi

    2015-03-01

    Full Text Available Os esteroides anabolizantes têm sido usados como arma terapêutica em diversas condições clínicas. Entretanto, o uso abusivo e indiscriminado, associado a outros suplementos nutricionais, tem gerado efeitos adversos graves. Relato do caso: Sexo masculino, 21 anos, admitido com náuseas, astenia, hiporexia, cefaleia e hipertensão arterial. Exames no sangue evidenciaram Cr: 3,9 mg/dl U:100 mg/dl e Cálcio total 14 mg/dl. Ultrassonografia e biópsia renal compatíveis com nefrocalcinose. Houve melhora gradativa da função renal e da calcemia após hidratação vigorosa e furosemida. Entretanto, após 1 ano, persistiram depósitos renais de cálcio e relação córticomedular reduzida ao ultrassom e creatinina estável em 1,4 mg/dl. Casos anteriores evidenciaram necrose tubular aguda e nefrite intersticial com poucos depósitos de cálcio no interstício renal. Nesse caso, encontramos nefrocalcinose acentuada associada à nefroesclerose. O objetivo deste estudo é relatar a ocorrência de injúria renal aguda com nefrocalcinose associada ao uso de esteroide anabolizante e oferecer uma revisão do assunto.

  1. A IMPORTÂNCIA DAS TAREFAS PIAGETIANAS APLICADAS NA EDUCAÇÃO INFANTIL

    Directory of Open Access Journals (Sweden)

    AZEVEDO, V.O.

    2015-12-01

    Full Text Available As provas desenvolvidas por Jean Piaget fazem-se presente a todo o momento na Educação Infantil, colaborando significativamente na aprendizagem de conceitos matemáticos e de outras áreas do conhecimento, favorecendo o desenvolvimento da capacidade de raciocinar, de comparar, de classificar, de argumentar do aluno. Estudos comprovam, que quando as tarefas piagetianas, como: seriação, classificação, inclusão de classe e outras, são trabalhadas com os alunos desde a Educação Infantil, estes adquirem um progresso na compreensão de conteúdos e atingem um estágio de desenvolvimento intelectual mais avançado ao longo do Ensino Fundamental. A presente pesquisa tem como objetivo investigar a importância das tarefas piagetianas desenvolvidas na Educação Infantil e averiguar as contribuições para o desenvolvimento do raciocínio lógico dos alunos do Infantil ao Ensino Fundamental. O trabalho consiste em uma pesquisa bibliográfica, de textos relacionados aos estudos de Piaget e seus seguidores, além do desenvolvimento das atividades piagetianas em turmas do maternal da Educação Infantil, no Centro Educacional Nossa Senhora Auxiliadora (CENSA, de Campos dos Goytacazes, utilizando materiais concretos e o próprio corpo da criança. Foi constatado que o uso das tarefas piagetianas desde a Educação infantil, como mecanismo para a aprendizagem e para o desenvolvimento do raciocínio lógico matemático, contribui também para o desenvolvimento de habilidades essenciais para outras áreas do conhecimento. Piaget enfatiza que o conhecimento, incluindo a capacidade de raciocinar logicamente, é construído através de um processo de interação/reflexão no qual o sujeito age sobre o objeto. Proporcionar atividades concretas e lúdicas às crianças é determinante para o desenvolvimento significativo de suas estruturas mentais necessárias a construção de conhecimentos matemáticos.

  2. Claudin-1和MMP-2在视网膜母细胞瘤中表达及其相关性%The expression and correlation of Claudin-1 and MMP-2 in retinoblastoma

    Institute of Scientific and Technical Information of China (English)

    杨洋; 邬黎青; 程波; 雷浪

    2013-01-01

    目的:探讨Claudin-1和MMP-2蛋白在视网膜母细胞瘤中的表达变化及其与视网膜母细胞瘤组织分化、视神经浸润和临床分期的相关性。方法采用免疫组化方法(MaxVisionTM)检测Claudin-1、MMP-2蛋白在45例视网膜母细胞瘤和15例正常视网膜组织石蜡标本的表达,运用卡方检验和Spearman等级相关检验分析Clandin-1和MMP-2在视网膜母细胞瘤组织中表达的相关性。结果(1)Claudin-1蛋白在视网膜母细胞瘤组织阳性表达明显低于在正常视网膜组织;在分化型组阳性表达显著高于未分化型组,P=0.015;在未侵犯视神经组阳性表达明显高于侵犯视神经组,P<0.001;在临床Ⅰ期、Ⅱ期、Ⅲ期组中各组间表达均具有统计学差异,P<0.01;不同性别组中Claudin-1表达没有统计学差异,P=0.661。(2)MMP-2蛋白在视网膜母细胞瘤中阳性表达明显高于正常视网膜组织细胞;在分化型组阳性表达中表达低于未分化型组,表达没有统计学差异,P=0.636;在侵犯视神经组阳性表达明显高于未侵犯视神经组,P=0.011;在临床Ⅰ期组、Ⅱ期和Ⅲ期各期之间中表达均具有统计学差异,P<0.05;在不同性别组中表达没有统计学差异,P=0.58。(3)在视网膜母细胞瘤中Claudin-1表达下降和MMP-2表达上升两者呈负相关(r=-0.537,P=0.023)。结论 Claudin-1表达水平与视网膜母细胞瘤细胞分化、视神经浸润和临床分期呈正相关;MMP-2表达水平与视网膜母细胞瘤视神经浸润和临床分期呈负相关。Clandin-1和MMP-2在视网膜母细胞瘤的视神经浸润与肿瘤发展起相反作用。%Objective To study the protein expression of Claudin-1 and MMP-2 protein in retinoblastoma and their correla-tion with retinoblastoma tissue differentiation,clinical optic nerve infiltration capacity and retinoblastoma staging. Methods Im-munohistochemistry was used to analyze the expression of

  3. Polymer science applied to petroleum production; Ciencia de polimeros aplicada a producao de petroleo

    Energy Technology Data Exchange (ETDEWEB)

    Lucas, Elizabete F.; Mansur, Claudia R.E.; Garreto, Maria S.E.; Honse, Siller O.; Mazzeo, Claudia P.P. [Universidade Federal do Rio de Janeiro/ Instituto de Macromoleculas/ Laboratorio de Macromoleculas e Coloides na Industria de Petroleo, Rio de Janeiro, RJ (Brazil)], e-mail: elucas@ima.ufrj.br

    2011-07-01

    The petroleum production comprises several operations, from well drilling to oil and water treatment, in which polymer science is applied. This work is focused in the phase behavior of asphaltenes that can be evaluated by precipitation tests and particle size determination. Recent researches show that the petroleum can be diluted with a specific model solvent, without causing any changes on asphaltenes phase behavior, and that a representative model system can be obtained if asphaltenes could be extracted using n-alkane as low as C1. The phase behavior of asphaltenes directly depends on the solubility parameter, which can be estimated for petroleum and asphaltenic fractions by microcalorimetry. More polar asphaltenes are not completely stabilized by less polar molecules, and this affects the stability of the A/O emulsions. There is a relationship between the amount of polar groups in the polymer chain and its capability in stabilizing/flocculating the asphaltenes, which interferes in the asphaltenes particle sizes. (author)

  4. Conserved retinoblastoma protein-binding motif in human cytomegalovirus UL97 kinase minimally impacts viral replication but affects susceptibility to maribavir

    Directory of Open Access Journals (Sweden)

    Chou Sunwen

    2009-01-01

    Full Text Available Abstract The UL97 kinase has been shown to phosphorylate and inactivate the retinoblastoma protein (Rb and has three consensus Rb-binding motifs that might contribute to this activity. Recombinant viruses containing mutations in the Rb-binding motifs generally replicated well in human foreskin fibroblasts with only a slight delay in replication kinetics. Their susceptibility to the specific UL97 kinase inhibitor, maribavir, was also examined. Mutation of the amino terminal motif, which is involved in the inactivation of Rb, also renders the virus hypersensitive to the drug and suggests that the motif may play a role in its mechanism of action.

  5. Control of cell proliferation, endoreduplication, cell size, and cell death by the retinoblastoma-related pathway in maize endosperm

    KAUST Repository

    Sabelli, Paolo A.

    2013-04-22

    The endospermof cereal grains is one of the most valuable products of modern agriculture. Cereal endosperm development comprises different phases characterized by mitotic cell proliferation, endoreduplication, the accumulation of storage compounds, and programmed cell death. Although manipulation of these processes could maximize grain yield, how they are regulated and integrated is poorly understood. We show that the Retinoblastoma-related (RBR) pathway controls key aspects of endosperm development in maize. Down-regulation of RBR1 by RNAi resulted in up-regulation of RBR3-type genes, as well as the MINICHROMOSOME MAINTENANCE 2-7 gene family and PROLIFERATING CELL NUCLEAR ANTIGEN, which encode essential DNA replication factors. Both the mitotic and endoreduplication cell cycles were stimulated. Developing transgenic endosperm contained 42-58% more cells and ~70% more DNA than wild type, whereas there was a reduction in cell and nuclear sizes. In addition, cell death was enhanced. The DNA content of mature endosperm increased 43% upon RBR1 downregulation, whereas storage protein content and kernel weight were essentially not affected. Down-regulation of both RBR1 and CYCLIN DEPENDENT KINASE A (CDKA);1 indicated that CDKA;1 is epistatic to RBR1 and controls endoreduplication through an RBR1- dependent pathway. However, the repressive activity of RBR1 on downstream targets was independent from CDKA;1, suggesting diversification of RBR1 activities. Furthermore, RBR1 negatively regulated CDK activity, suggesting the presence of a feedback loop. These results indicate that the RBR1 pathway plays a major role in regulation of different processes during maize endosperm development and suggest the presence of tissue/organlevel regulation of endosperm/seed homeostasis.

  6. Inactivation of retinoblastoma protein does not overcome the requirement for human cytomegalovirus UL97 in lamina disruption and nuclear egress.

    Science.gov (United States)

    Reim, Natalia I; Kamil, Jeremy P; Wang, Depeng; Lin, Alison; Sharma, Mayuri; Ericsson, Maria; Pesola, Jean M; Golan, David E; Coen, Donald M

    2013-05-01

    Human cytomegalovirus (HCMV) encodes one conventional protein kinase, UL97. During infection, UL97 phosphorylates the retinoblastoma tumor suppressor protein (pRb) on sites ordinarily phosphorylated by cyclin-dependent kinases (CDK), inactivating the ability of pRb to repress host genes required for cell cycle progression to S phase. UL97 is important for viral DNA synthesis in quiescent cells, but this function can be replaced by human papillomavirus type 16 E7, which targets pRb for degradation. However, viruses in which E7 replaces UL97 are still defective for virus production. UL97 is also required for efficient nuclear egress of viral nucleocapsids, which is associated with disruption of the nuclear lamina during infection, and phosphorylation of lamin A/C on serine 22, which antagonizes lamin polymerization. We investigated whether inactivation of pRb might overcome the requirement of UL97 for these roles, as pRb inactivation induces CDK1, and CDK1 phosphorylates lamin A/C on serine 22. We found that lamin A/C serine 22 phosphorylation during HCMV infection correlated with expression of UL97 and was considerably delayed in UL97-null mutants, even when E7 was expressed. E7 failed to restore gaps in the nuclear lamina seen in wild-type but not UL97-null virus infections. In electron microscopy analyses, a UL97-null virus expressing E7 was as impaired as a UL97-null mutant in cytoplasmic accumulation of viral nucleocapsids. Our results demonstrate that pRb inactivation is insufficient to restore efficient viral nuclear egress of HCMV in the absence of UL97 and instead argue further for a direct role of UL97 in this stage of the infectious cycle.

  7. Study of polymorphisms in the TP53 and RB1 genes in children with retinoblastoma in northern Mexico

    Science.gov (United States)

    Nares-Cisneros, Jesús; Cárdenas-Hernández, Rubén I.; Jaramillo-Rodríguez, Yolanda; Zambrano-Galván, Graciela

    2017-01-01

    Purpose To determine the frequency and association of polymorphisms in the TP53 and RB1 genes with clinical characteristics in a group of children with retinoblastoma (RB) in northern Mexico. Methods A prospective, longitudinal, and analytical study of 11 patients diagnosed with RB was conducted. Endpoint PCR and high-resolution real-time PCR were performed. Chi-square and Student t tests were used to evaluate associations between variables. Allelic frequencies, as well as genotypic and Hardy–Weinberg equilibriums, were evaluated using Guo and Thompson’s method. Results We found a statistically significant difference between the polymorphism RB1-GG/rs9568036 and tumor chemoresistance (p<0.05). The allelic variants RB1-AA and AG/rs9568036 were determined to be associated with tumor chemosensitivity (p<0.05). A statistically significant relation between the polymorphism RB1-GG/rs9568036 and males (p = 0.0386), rate ratio (RR) = 2.0 (95% confidence interval [CI] = 0.76–5.32), as well as between the allelic variants RB1-AA and AG/rs9568036 and females (p = 0.0027), RR = 8.0 (95% CI = 1.28–50.04), was observed. We also observed a statistically significant association between the rs1042522 polymorphism in the TP53 gene and unilateral presentation of the disease. Conclusions The rs9568036 polymorphism in the RB1 gene and the allelic variants can be associated with type of response to medical therapy and associated with male sex, while the allelic variant rs1042522 polymorphism in the TP53 gene is associated with the unilateral presentation of the disease in a group of Mexican children with RB. PMID:28210099

  8. Histone demethylase retinoblastoma binding protein 2 is overexpressed in hepatocellular carcinoma and negatively regulated by hsa-miR-212.

    Directory of Open Access Journals (Sweden)

    Xiuming Liang

    Full Text Available BACKGROUND: The H3K4 demethylase retinoblastoma binding protein 2 (RBP2 is involved in the pathogenesis of gastric cancer, but its role and regulation in hepatocellular carcinoma (HCC is unknown. We determined the function of RBP2 and its regulation in HCC in vitro and in human tissues. METHODS: We analyzed gene expression in 20 specimens each of human HCC and normal liver tissue by quantitative real-time PCR and immunohistochemistry. Proliferation was analyzed by foci formation and senescence by β-galactosidase staining. Promoter activity was detected by luciferase reporter assay. RESULTS: The expression of RBP2 was stronger in cancerous than non-cancerous tissues, but that of its binding microRNA, Homo sapiens miR-212 (hsa-miR-212, showed an opposite pattern. SiRNA knockdown of RBP2 significantly upregulated cyclin-dependent kinase inhibitors (CDKIs, with suppression of HCC cell proliferation and induction of senescence. Overexpression of hsa-miR-212 suppressed RBP2 expression, with inhibited cell proliferation and induced cellular senescence, which coincided with upregulated CDKIs; with low hsa-miR-212 expression, CDKIs were downregulated in HCC tissue. Inhibition of hsa-miR-212 expression upregulated RBP2 expression. Luciferase reporter assay detected the direct binding of hsa-miR-212 to the RBP2 3' UTR. CONCLUSIONS: RBP2 is overexpressed in HCC and negatively regulated by hsa-miR-212. The hsa-miR-212-RBP2-CDKI pathway may be important in the pathogenesis of HCC.

  9. Retinoblastoma-binding protein 2 (RBP2) is frequently expressed in neuroendocrine tumors and promotes the neoplastic phenotype.

    Science.gov (United States)

    Maggi, E C; Trillo-Tinoco, J; Struckhoff, A P; Vijayaraghavan, J; Del Valle, L; Crabtree, J S

    2016-08-22

    Neuroendocrine tumors (NETs), which can have survival rates as low as 4%, currently have limited therapeutic interventions available highlighting the dire need for the identification of novel biological targets for use as new potential drug targets. One such potential target is retinoblastoma-binding protein 2 (RBP2), an H3K4 demethylase whose overexpression has been linked to cancer formation and metastasis in non-endocrine tumor types. We measured RBP2 mRNA and protein levels in enteropancreatic NETs by measuring RBP2 in matched human normal and NET tissue samples. Further, proliferation, migration, invasion and colony formation assays were performed in the physiologically relevant NET cell lines βlox5, H727 and QGP-1 to understand the role of RBP2 and its demethylase activity on end points of tumorigenesis. Our data indicate a strong correlation between RBP2 mRNA and protein expression in NET specimens. RBP2 was overexpressed relative to tissue-matched normal controls in 80% of the human tumors measured. In vitro studies showed RBP2 overexpression significantly increased proliferation, migration, invasion and colony formation, whereas knockdown significantly decreases the same parameters in a demethylase-independent manner. The cell cycle inhibitors p21 and p57 decreased with RBP2 overexpression and increased upon its depletion, suggesting a regulatory role for RBP2 in cellular proliferation. Taken together, our results support the hypothesis that the aberrant overexpression of RBP2 is a frequent contributing factor to tumor formation and metastasis in enteropancreatic NETs.

  10. More epigenetic hits than meets the eye: microRNAs and genes associated with the tumorigenesis of retinoblastoma

    Directory of Open Access Journals (Sweden)

    Adriana H.O. Reis

    2012-12-01

    Full Text Available Retinoblastoma (RB, a childhood neoplasia of the retinoblasts, can occur unilaterally or bilaterally, with one or multiple foci per eye. RB is associated with somatic loss-of-function of both alleles of the tumor suppressor gene RB1. Hereditary forms emerge due to germline loss-of-function mutations in RB1 alleles. RB has long been the prototypic ‘‘model’’ cancer ever since Knudson’s ‘‘two-hit’’ hypothesis. However, a simple two-hit model for RB is challenged by an increasing number of studies documenting additional hits that contribute to RB development. Here we review the genetics and epigenetics of RB with a focus on the role of small noncoding RNAs (microRNAs and on novel findings indicating the relevance of DNA methylation in the development and prognosis of this neoplasia. Studies point to an elaborated landscape of genetic and epigenetic complexity, in which a number of events and pahtways play crucial roles in the origin and prognosis of RB. These include roles for microRNAs, inprinted loci, and parent-of-origin contributions to RB1 regulation and RB progression. This complexity is also manifested in the structure of the RB1 locus itself: it includes numerous repetitive DNA segments and retrotransposon insertion elements, some of which are actively transcribed from the RB1 locus. Altogether, we conclude that RB1 loss of function represents the tip of an iceberg of events that determine RB development, progression, severity, and disease risk. Comprehensive assessment of personalized RB risk will require genetic and epigenetic evaluations beyond RB1 protein coding sequences.

  11. RNAi mediated acute depletion of Retinoblastoma protein (pRb promotes aneuploidy in human primary cells via micronuclei formation

    Directory of Open Access Journals (Sweden)

    Iovino Flora

    2009-11-01

    Full Text Available Abstract Background Changes in chromosome number or structure as well as supernumerary centrosomes and multipolar mitoses are commonly observed in human tumors. Thus, centrosome amplification and mitotic checkpoint dysfunctions are believed possible causes of chromosomal instability. The Retinoblastoma tumor suppressor (RB participates in the regulation of synchrony between DNA synthesis and centrosome duplication and it is involved in transcription regulation of some mitotic genes. Primary human fibroblasts were transfected transiently with short interfering RNA (siRNA specific for human pRb to investigate the effects of pRb acute loss on chromosomal stability. Results Acutely pRb-depleted fibroblasts showed altered expression of genes necessary for cell cycle progression, centrosome homeostasis, kinetochore and mitotic checkpoint proteins. Despite altered expression of genes involved in the Spindle Assembly Checkpoint (SAC the checkpoint seemed to function properly in pRb-depleted fibroblasts. In particular AURORA-A and PLK1 overexpression suggested that these two genes might have a role in the observed genomic instability. However, when they were post-transcriptionally silenced in pRb-depleted fibroblasts we did not observe reduction in the number of aneuploid cells. This finding suggests that overexpression of these two genes did not contribute to genomic instability triggered by RB acute loss although it affected cell proliferation. Acutely pRb-depleted human fibroblasts showed the presence of micronuclei containing whole chromosomes besides the presence of supernumerary centrosomes and aneuploidy. Conclusion Here we show for the first time that RB acute loss triggers centrosome amplification and aneuploidy in human primary fibroblasts. Altogether, our results suggest that pRb-depleted primary human fibroblasts possess an intact spindle checkpoint and that micronuclei, likely caused by mis-attached kinetochores that in turn trigger

  12. Escala revisada de Valores relativos ao Trabalho - EVT-R Revised Work Values Scale - WVS-R

    Directory of Open Access Journals (Sweden)

    Juliana Barreiros Porto

    2010-04-01

    Full Text Available Os valores do trabalho são os princípios que guiam a vida do indivíduo no trabalho. No Brasil, uma escala que já foi amplamente utilizada em pesquisas é a Escala de Valores relativos ao Trabalho (EVT. O objetivo do primeiro estudo foi realizar o teste confirmatório da estrutura empírica da escala. Uma amostra de 790 profissionais respondeu ao instrumento. Os resultados apontaram a adequação do modelo, mas a representação dos tipos motivacionais foi limitada. Para suprir esta lacuna, a escala foi revisada e validada no segundo estudo. A nova escala foi aplicada em uma amostra de 412 trabalhadores. Os resultados da análise fatorial e do escalonamento multidimensional apontam que houve melhoras significativas na estrutura empírica da escala. As implicações são discutidas.Work values are guiding principles in the work life of a person. In Brazil, a broadly used scale is the Work Values Scale (WVS. The purpose of the first study was to do a confirmatory test of the empirical structure of WVS. A sample of 790 workers answered this instrument of research. The results showed a good fit of the structure, but also indicated a poor representation of some motivational types. The scale was revised and validated in the second study. More items were developed and the new scale was applied in a sample of 412 workers. The factor analysis and multidimensional scaling results showed an improvement of the empirical structure. Implications are discussed.

  13. Aproximación al léxico de la ciencia aplicada en el Renacimiento hispano

    Directory of Open Access Journals (Sweden)

    Mancho Duque, M.ª J.

    2003-12-01

    Full Text Available In this study we analyze the appearance of technical vocabulary during the spanish Renaissance. Some of the main linguistic characteristics are taken into account from various levels: phonetics, morphology and semantics, with a series of specific examples. Several examples of loanwords are studied from either classic or modern language, chosen from the most prestigious languages of the time.

    En este trabajo se enfoca el surgimiento del vocabulario especializado en el campo de la ciencia aplicada durante el período del renacimiento español. Se analizan algunas de las principales características desde los planos gráfico-fonético, morfológico y semántico, de las que se aportan ejemplos concretos, y se recogen testimonios de préstamos procedentes, tanto de lenguas clásicas, como de otras de prestigio cultural en la época.

  14. Teoría de categorías aplicada a la programación funcional con Agda.

    OpenAIRE

    Villa-Isaza, Juan Pedro

    2011-01-01

    Teoría de categorías aplicada a la programación funcional con Agda es una implementación de algunos conceptos de la programación funcional que están basados o han sido tomados de la teoría de categorías (categorías, functores, functores aplicativos, mónadas...). En este artículo se analizan las diferencias entre la definición formal de un functor y su definición en el lenguaje de programación Haskell, y se implementa un functor en el asistente de pruebas Agda que soluciona dichas diferencias....

  15. LGS-AO Imaging of Every Kepler Planet Candidate: the Robo-AO KOI Survey

    Science.gov (United States)

    Baranec, Christoph; Law, Nicholas; Morton, Timothy; Ziegler, Carl; Nofi, Larissa; Atkinson, Dani; Riddle, Reed

    2015-12-01

    The Robo-AO Kepler Planetary Candidate Survey is observing every Kepler planet candidate host star with laser adaptive optics imaging, to search for blended nearby stars which may be physically associated companions and/or responsible for transit false positives. We will present the results from searching for companions around over 3,000 Kepler planet hosts in 2012-2015. We will describe our first data release covering 715 planet candidate hosts, and give a preview of ongoing results including improved statistics on the likelihood of false positive planet detections in the Kepler dataset, many new planets in multiple star systems, and new exotic multiple star systems containing Kepler planets. We will also describe the automated Robo-AO survey data reduction methods, including a method of using the large ensemble of target observations as mutual point-spread-function references, along with a new automated companion-detection algorithm designed for extremely large adaptive optics surveys. Our first data release covered 715 objects, searching for companions from 0.15” to 2.5” separation with contrast up to 6 magnitudes. We measured the overall nearby-star-probability for Kepler planet candidates to be 7.4+/-1.0%, and we will detail the variations in this number with stellar host parameters. We will also discuss plans to extend the survey to other transiting planet missions such as K2 and TESS as Robo-AO is in the process of being re-deployed to the 2.1-m telescope at Kitt Peak for 3 years and a higher-contrast Robo-AO system is being developed for the 2.2-m UH telescope on Maunakea.

  16. Review of AO calibrations, or how to best educate your AO system

    Science.gov (United States)

    Kolb, Johann

    2016-07-01

    If the Real-Time Computer is the heart of an AO system, the Wavefront Sensor (WFS) its eyes, the Deformable Mirror (DM) its hands and the control strategy its nervous system, the sum of all those parts is made into a harmonious entity thanks to calibrations. This paper does not have the ambition to provide an overview of all the currently existing calibration strategies, but rather to focus on a few challenging problems and their recent evolution in the era of adaptive telescopes, mostly based on the experience of ESO's Adaptive Optics Instruments in general and the AO Facility in particular. Single most important calibration in post-focal AO system, the recording of the Interaction Matrix (IM) between WFS and DM has since long evolved to use fast modulation techniques, has shown to be feasible on-sky and is now almost free from measurements thanks to its pseudo-synthetic generation, quasi-mandatory solution in an adaptive telescope. Pseudo- because it requires an unprecedented knowledge of the components' characteristics, especially the WFS, DM and the optical registration between the two. Bigger telescopes and the use of Laser Guide Stars (LGS) also mean that the properties of the system will change in time and thus need to be constantly updated thanks to online diagnosis tools for spot size measurement, atmosphere monitoring, Wavefront Sensing and control optimization. New loops come into play like the one to minimize LGS Jitter and the one taking over the telescope active optics by means of offloading the DM low orders, and they all require calibration. More calibration means more time and one has to carefully balance the calibrations that require precious telescope night time, day time or for the best, no telescope time at all. Their importance sometimes underestimated, calibrations have repeatedly shown to be a vital part in the optimum functioning of present and future AO systems.

  17. Adesão ao tratamento da Hanseníase: dificuldades inerentes aos portadores

    Directory of Open Access Journals (Sweden)

    Izaildo Tavares Luna

    2010-12-01

    Full Text Available Esta pesquisa, realizada em Guaiúba, CE, Brasil, objetivou identificar as dificuldades de adesão ao tratamento pelos portadores de hanseníase, relacionando os fatores associados. Estudo qualitativo, cujos dados foram coletados a partir de uma visita domiciliária com roteiro de entrevista contendo sete questões norteadoras. Participaram pacientes com diagnóstico de hanseníase e histórico de não adesão ao tratamento. Utilizou-se o Modelo de Atividade de Vida de Roper, Logan e Tierney. As falas dos sujeitos foram categorizadas de acordo com as doze atividades de vida. A utilização do modelo possibilitou uma abordagem holística sobre as barreiras vivenciadas pelo hanseniano na adesão ao tratamento medicamentoso, de modo a conhecer as atividades de vida destes pacientes para possível adequação de ações voltadas a essa clientela.

  18. ABORDAGEM GEOAMBIENTAL APLICADA À ANÁLISE DA VULNERABILIDADE E DOS RISCOS EM AMBIENTES URBANOS

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    Jader de Oliveira Santos

    2014-08-01

    Full Text Available A sociedade, ao apropriar-se do território e dos recursos naturais, promove significativas modificações nas paisagens, desencadeando uma série de problemas socioambientais. Nas áreas urbanas essas consequências são mais evidentes, em virtude, principalmente, da ocupação desordenada dos ambientes mais vulneráveis, que gera impactos e riscos emergentes. A pretensão deste texto é apresentar a análise geoambiental como instrumento de investigação da vulnerabilidade e dos riscos ambientais em sistemas marcados pela urbanização. Para tanto, utiliza-se como estudo de caso a bacia hidrográfica do rio Cocó, localizada na Região Metropolitana de Fortaleza (CE. Os resultados obtidos mostram a eficácia da aplicação desse tipo de abordagem para a compreensão da distribuição espacial dos riscos e identificação de territórios mais susceptíveis a esses problemas.

  19. Técnicas moleculares aplicadas à microbiologia de alimentos = Molecular techniques applied to food microbiology

    Directory of Open Access Journals (Sweden)

    Eliezer Ávila Gandra

    2008-01-01

    Full Text Available A partir da década de 80, as técnicas moleculares começaram a ser utilizadas como uma alternativa aos métodos fenotípicos, tradicionalmente, utilizados em microbiologia de alimentos. Foi acelerada esta substituição com advento da descoberta da reação em cadeia da polimerase (polymerase chain reaction – PCR. Este artigo tem por objetivo revisar as principais técnicas moleculares utilizadas como ferramentas na microbiologia de alimentos, desde as, inicialmente, desenvolvidas, como a análise do perfil plasmidial, até as mais contemporâneas como o PCR em tempo real, discutindo as características, vantagens e desvantagens destas técnicas, avaliando a potencialidade destas para suprir as limitações das técnicas tradicionais.Beginning in the 1980s, molecular techniques became an alternative to the traditionally used phenotypic methods in food microbiology. With the advent of the polymerase chain reaction technique, this substitution was speed up. This article had as objective to review the main molecular techniques used as tools in food microbiology, from plasmidial profile analysis to contemporary techniques such as the real-time PCR. The characteristics, advantages anddisadvantages of these techniques are discussed, by evaluating the potential of these techniques to overcome the limitations of traditional techniques.

  20. Directly Imaging Planets with SCExAO: First Results

    Science.gov (United States)

    Currie, Thayne M.; Guyon, Olivier; Jovanovic, Nemanja; Lozi, Julien; Tamura, Motohide; Kudo, Tomoyuki; Uyama, Taichi; Garcia, Eugenio

    2017-01-01

    We present the first science results from the newly commissioned Subaru Coronagraphic Extreme Adaptive Optics project, an experimental system dedicated to image faint jovian planets around nearby stars. SCExAO is now achieving true extreme AO capability. We describe the typical performance of SCExAO, the first images of benchmark exoplanets and planet-forming disks, and SCExAO’s first science results. Finally, we briefly chart the path forward for SCExAO to achieve its full scientific capability, including imaging mature planets in reflected light.

  1. The N-terminal domain of the Drosophila retinoblastoma protein Rbf1 interacts with ORC and associates with chromatin in an E2F independent manner.

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    Joseph Ahlander

    Full Text Available BACKGROUND: The retinoblastoma (Rb tumor suppressor protein can function as a DNA replication inhibitor as well as a transcription factor. Regulation of DNA replication may occur through interaction of Rb with the origin recognition complex (ORC. PRINCIPAL FINDINGS: We characterized the interaction of Drosophila Rb, Rbf1, with ORC. Using expression of proteins in Drosophila S2 cells, we found that an N-terminal Rbf1 fragment (amino acids 1-345 is sufficient for Rbf1 association with ORC but does not bind to dE2F1. We also found that the C-terminal half of Rbf1 (amino acids 345-845 interacts with ORC. We observed that the amino-terminal domain of Rbf1 localizes to chromatin in vivo and associates with chromosomal regions implicated in replication initiation, including colocalization with Orc2 and acetylated histone H4. CONCLUSIONS/SIGNIFICANCE: Our results suggest that Rbf1 can associate with ORC and chromatin through domains independent of the E2F binding site. We infer that Rbf1 may play a role in regulating replication directly through its association with ORC and/or chromatin factors other than E2F. Our data suggest an important role for retinoblastoma family proteins in cell proliferation and tumor suppression through interaction with the replication initiation machinery.

  2. White spot syndrome virus IE1 and WSV056 modulate the G1/S transition by binding to the host retinoblastoma protein.

    Science.gov (United States)

    Ran, Xiaozhuo; Bian, Xiaofang; Ji, Yongchang; Yan, Xiumin; Yang, Feng; Li, Fang

    2013-12-01

    DNA viruses often target cellular proteins to modulate host cell cycles and facilitate viral genome replication. However, whether proliferation of white spot syndrome virus (WSSV) requires regulation of the host cell cycle remains unclear. In the present study, we show that two WSSV paralogs, IE1 and WSV056, can interact with Litopenaeus vannamei retinoblastoma (Rb)-like protein (lv-RBL) through the conserved LxCxE motif. Further investigation revealed that IE1 and WSV056 could also bind to Drosophila retinoblastoma family protein 1 (RBF1) in a manner similar to how they bind to lv-RBL. Using the Drosophila RBF-E2F pathway as a model system, we demonstrated that both IE1 and WSV056 could sequester RBF1 from Drosophila E2F transcription factor 1 (E2F1) and subsequently activate E2F1 to stimulate the G1/S transition. Our findings provide the first evidence that WSSV may regulate cell cycle progression by targeting the Rb-E2F pathway.

  3. Preliminary Experience in Treatment of Papillary and Macular Retinoblastoma: Evaluation of Local Control and Local Complications After Treatment With Linear Accelerator-Based Stereotactic Radiotherapy With Micromultileaf Collimator as Second-Line or Salvage Treatment After Chemotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Pica, Alessia, E-mail: Alessia.Pica@chuv.ch [Department of Radiation Oncology, Centre Hospitalier Universitaire Vaudois, Lausanne (Switzerland); Moeckli, Raphael [University Institute for Radiation Physics, Centre Hospitalier Universitaire Vaudois, Lausanne (Switzerland); Balmer, Aubin [Department of Radiation Oncology, Jules Gonin Eye Hospital, Lausanne (Switzerland); Beck-Popovic, Maja [Unit of Pediatric Oncology, University of Lausanne, Lausanne (Switzerland); Chollet-Rivier, Madeleine [Department of Anesthesiology, University of Lausanne, Lausanne (Switzerland); Do, Huu-Phuoc [University Institute for Radiation Physics, Centre Hospitalier Universitaire Vaudois, Lausanne (Switzerland); Weber, Damien C. [Department of Radiation Oncology, Geneva University Hospital, University of Geneva, Geneva (Switzerland); Munier, Francis L. [Department of Radiation Oncology, Jules Gonin Eye Hospital, Lausanne (Switzerland)

    2011-12-01

    Purpose: To determine the local control and complication rates for children with papillary and/or macular retinoblastoma progressing after chemotherapy and undergoing stereotactic radiotherapy (SRT) with a micromultileaf collimator. Methods and Materials: Between 2004 and 2008, 11 children (15 eyes) with macular and/or papillary retinoblastoma were treated with SRT. The mean age was 19 months (range, 2-111). Of the 15 eyes, 7, 6, and 2 were classified as International Classification of Intraocular Retinoblastoma Group B, C, and E, respectively. The delivered dose of SRT was 50.4 Gy in 28 fractions using a dedicated micromultileaf collimator linear accelerator. Results: The median follow-up was 20 months (range, 13-39). Local control was achieved in 13 eyes (87%). The actuarial 1- and 2-year local control rates were both 82%. SRT was well tolerated. Late adverse events were reported in 4 patients. Of the 4 patients, 2 had developed focal microangiopathy 20 months after SRT; 1 had developed a transient recurrence of retinal detachment; and 1 had developed bilateral cataracts. No optic neuropathy was observed. Conclusions: Linear accelerator-based SRT for papillary and/or macular retinoblastoma in children resulted in excellent tumor control rates with acceptable toxicity. Additional research regarding SRT and its intrinsic organ-at-risk sparing capability is justified in the framework of prospective trials.

  4. Expression of retinoblastoma protein and P16 proteins in classic Hodgkin lymphoma : relationship with expression of p53 and presence of Epstein-Barr virus in the regulation of cell growth and death

    NARCIS (Netherlands)

    Kim, LH; Peh, SC; Poppema, S

    2006-01-01

    Deregulation of several genes involved in cell cycle control has been reported in classic Hodgkin lymphoma (cHL). This study aimed to investigate the expression of tumor suppressor proteins INK4A (P16(INK4A), retinoblastoma protein, and p53) in cHL in relation to the proliferation and apoptosis of H

  5. Desafio aos Gestores de Unidades de Informação para Implementar o Intraempreendedorismo e o Empowerment Desafíe para los gerentes de unidades de información llevar a cabo el intrapreneurship y empowerment

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    Roberta Moraes de Bem

    2012-05-01

    Full Text Available Introdução: A gestão das organizações contemporâneas exige ferramentas gerenciais adequadas ao seu tempo. Essas ferramentas estão disponíveis ao gestor de unidades de informação e podem ser aplicadas na gestão desse tipo de sistema organizacional. Objetivo: Apresentar os conceitos de intraempreendedorismo e de empowerment como os principais elementos do clima organizacional necessários ao desenvolvimento do espírito empreendedor. Metodologia: O instrumento de coleta de dados foi o questionário, aplicado em 2010 para servidores de uma biblioteca universitária. No universo da pesquisa de 56 sujeitos, 36% dos questionários foram respondidos.Resultados: Os resultados permitem inferir que, de um total de dez características empreendedoras, três delas (estabelecimento de metas, riscos calculados e persuasão e redes de contatos foram consideradas como pontos fracos no grupo analisado. A constatação de que os profissionais pesquisados têm, no nível grupal, grau positivo favorável ao empreendedorismo - possuindo 70% de características empreendedoras pessoais positivas sugere que os mesmos podem auxiliar na inovação da unidade de informação se os gestores criarem um ambiente favorável ao intraempreendedorismo. Quanto ao clima organizacional, este foi considerado favorável à implementação do empowerment. Conclusões: As conclusões finais revelam que a unidade de informação pode ser beneficiada com a aplicação do empowerment, de forma a promover o espírito empreendedor, estando aberta para mudanças culturais rumo a uma Administração Empreendedora.

  6. Medidas socioeducativas: cartas ao reinado do saber

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    Gislei Domingas Romanzini Lazzarotto

    2014-09-01

    Full Text Available O estudo problematiza a experiência desenvolvida na defensoria interdisciplinar de adolescentes em conflito com a lei num programa de extensão acadêmica da Univewrsidade Federal do Rio Grande do Sul, no contexto de políticas públicas brasileiras. A metodologia do estudo consiste em analisar as narrativas sobre o trabalho da equipe de defensoria interdisciplinar discutindo as experiências ali vivenciadas com um olhar crítico sobre as práticas desenvolvidas no atendimento de adolescentes que cumprem medida socioeducativa por terem cometido ato infracional. A análise conceitual é orientada pelo diálogo com o filósofo Michel Foucault, especial em seu texto " La vie des hommes infâmes”, mapeando as relações de poder e saber que regulam as práticas institucionais e visam ao controle social dos indivíduos. A análise possibilita uma reflexão sobre as ligações que são estabelecidas entre os saberes do direito, da psicologia e da pedagogia, produzindo práticas que tendem a regular e prescrever a vida de adolescentes no contexto de atendimento socioeducativo, numa perspectiva de judicialização da vida contemporânea.

  7. Toxicologia do tolueno: aspectos relacionados ao abuso

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    Letícia M.K. Forster

    1994-04-01

    Full Text Available O tolueno está presente em muitos produtos de uso doméstico e industrial e é o principal solvente envolvido no abuso de substâncias e na exposição ocupacional. O problema mais grave no estudo de patologias relacionadas ao tolueno é que este está geralmente associado, em suas preparações comerciais, a outras substâncias. O potencial tóxico do tolueno foi abordado nos seguintes aspectos: parâmetros farmacológicos; características físico-químicas; exposição; estudos clínicos; diagnóstico; pesquisa experimental; tolerância e dependência; efeitos agudos e crônicos; neurotoxicidade; teratogenicidade; doenças psiquiátricas; carcinogenicidade e tratamento. Conclui-se ser de grande importância e urgência que se realizem estudos clínicos com amostras maiores para definição mais precisa das conseqüências do uso crônico

  8. Toxicologia do tolueno: aspectos relacionados ao abuso

    Directory of Open Access Journals (Sweden)

    Forster Letícia M.K.

    1994-01-01

    Full Text Available O tolueno está presente em muitos produtos de uso doméstico e industrial e é o principal solvente envolvido no abuso de substâncias e na exposição ocupacional. O problema mais grave no estudo de patologias relacionadas ao tolueno é que este está geralmente associado, em suas preparações comerciais, a outras substâncias. O potencial tóxico do tolueno foi abordado nos seguintes aspectos: parâmetros farmacológicos; características físico-químicas; exposição; estudos clínicos; diagnóstico; pesquisa experimental; tolerância e dependência; efeitos agudos e crônicos; neurotoxicidade; teratogenicidade; doenças psiquiátricas; carcinogenicidade e tratamento. Conclui-se ser de grande importância e urgência que se realizem estudos clínicos com amostras maiores para definição mais precisa das conseqüências do uso crônico

  9. Electromagnetic DM technology meets future AO demands

    Science.gov (United States)

    Hamelinck, Roger; Rosielle, Nick; Steinbuch, Maarten; Doelman, Niek

    New deformable mirror technology is developed by the Technische Universiteit Eindhoven, Delft University of Technology and TNO Science and Industry. Several prototype adaptive deformable mirrors are realized mirrors, up to 427 actuators and ∅150mm diameter, with characteristics suitable for future AO systems. The prototypes consist of a 100µm thick, continuous facesheet on which low voltage, electromagnetic, push-pull actuators impose out-of-plane displacements. The variable reluctance actuators with ±10µm stroke and nanometer resolution are located in a standard actuator module. Each module with 61 actuators connects to a single PCB with dedicated, 16 bit, PWM based, drivers. A LVDS multi-drop cable connects up to 32 actuator modules. With the actuator module, accompanying PCB and multi-drop system the deformable mirror technology is made modular in its mechanics and electronics. An Ethernet-LVDS bridge enables any commercial PC to control the mirror using the UDP standard. Latest results of the deformable mirror technology development are presented.

  10. Adesao ao guia alimentar para populacao brasileira

    Directory of Open Access Journals (Sweden)

    Eliseu Verly Junior

    2013-12-01

    Full Text Available OBJETIVO : Analisar a adesão ao Guia Alimentar para População Brasileira. MÉTODOS : Amostra composta por participantes do Inquérito de Saúde de São Paulo (n = 1.661 que preencheram dois recordatórios de 24 horas. Foi utilizado modelo bivariado de efeito misto para a razão entre o consumo de energia do grupo de alimentos e o consumo calórico total. A razão estimada foi utilizada para calcular o percentual de indivíduos com consumo abaixo ou acima da recomendação. RESULTADOS : Pelo menos 80,0% da população consome abaixo do recomendado para: leite e derivados; frutas e sucos de frutas; e cereais, tubérculos e raízes; aproximadamente 60,0% para legumes e verduras; 30,0% para feijões; e 8,0% para carnes e ovos. Adolescentes apresentaram a maior inadequação para legumes e verduras (90,0%, e o estrato de maior renda foi associado à menor inadequação para óleos, gorduras e sementes oleaginosas (57,0%. CONCLUSÕES : Foi observado consumo inadequado dos grupos de alimentos relacionados com aumento do risco de doenças crônicas.

  11. Brachyuran Crabs collected at Curaçao

    NARCIS (Netherlands)

    Rathbun, Mary J.

    1924-01-01

    In 1920 a report ¹) on Dr. BOEKE’S collection of crabs and shrimps from Curaçao and other Dutch West Indian Islands was published. Dr. VAN DER HORST’S collection forms an important addition to the fauna of Curaçao, especially as to the Xanthids and Majids, or spider crabs. Four new species were disc

  12. The Birds of Aruba, Curaçao, and Bonaire

    NARCIS (Netherlands)

    Voous, K.H.

    1957-01-01

    This is the second and concluding part of a report on the birds of the Netherlands Antilles, the first part dealt with the birds of St. Martin, Saba, and St. Eustatius (Studies fauna Curaçao Car. Is. 6, no. 25, 1955, p. 1-82). The present part will give a full account of the birds of Aruba, Curaçao,

  13. POTENCIALIDADES E ESPAÇOS AO EMPREENDEDORISMO

    Directory of Open Access Journals (Sweden)

    Adriano Lago

    2008-12-01

    Full Text Available A criação de negócios é apontada como uma das causas da prosperidade de um local, região, estado ou de um país. É neste sentido que o empreendedorismo tem recebido destaque nos últimos anos, como promotor de novos negócios. Captar, descrever e analisar o fenômeno do empreendedorismo é, portanto, necessário para o desenho de ações de promoção do desenvolvimento. Assim, o presente estudo aborda o empreendedorismo na piscicultura, tomando como base três casos analisados: uma propriedade com rizipiscicultura, uma com piscicultura de ciclo completo inclusive com frigorífico, e outra onde a piscicultura desenvolve-se através de um pesque-pague, com vistas a proporcionar o turismo rural. Para fins de pesquisa, entrevistaram-se os proprietários de cada estabelecimento na busca dos dados necessários para a construção de uma abordagem consistente da temática proposta, ou seja, identificar a presença do empreendedorismo e suas características particulares em cada propriedade analisada. Verificou-se que determinadas características empreendedoras são comuns aos três casos analisados: habilidade para identificar oportunidades; busca de conhecimento de sua área de atuação; senso de organização; disposição para tomar decisões; capacidade de liderança; talento para empreender; otimismo e tino empresarial; ser estrategista, além do dinamismo. Portanto, foi possível observar que o perfil empreendedor dos proprietários de cada negócio tornou possível a transformação de idéias e problemas em oportunidades reais de negócio.

  14. WIYN active optics: a platform for AO

    Science.gov (United States)

    Code, Arthur D.; Claver, Charles F.; Goble, Larry W.; Jacoby, George H.; Sawyer, David G.

    1998-09-01

    The WIYN 3.5 meter telescope is situated on the southwest ridge of Kitt Peak yielding excellent atmosphere seeing conditions. As such, the telescope and enclosure design was directed towards exploiting this feature. The primary mirror was spun cast and figured by the Steward Observatory Mirror Laboratory and the secondary mirror by Contraves. In both cases the performance exceeded the design specifications. The borosilicate primary is actively temperature controlled to within 0.2 C of the desired temperature, typically 0.5 degrees C below the ambient air. The telescope structure is also temperature controlled and the enclosure is opened to the outside ion all sides, which all heat sources are vented to ducts carrying air downwind of the facility. The primary mirror is actively controlled for low order aberrations by 66 axial actuators which are adjusted open loop via force matrix look-up tables and closed loop via real-time wavefront curvature sensing measurements. The active optics also included real-time collimation and focus control. The telescope drive and guider are capable of providing tracking to a few hundredths of a second of arc. By employing active telescope control at this level, it is possible to maintain telescope and local wavefront distortion to a level where atmospheric effects dominate the image quality. Since a significant fraction of the power in the atmospheric disturbances is contained in image motion the first step in adaptive optics control will be simple tip tilt. Studies of higher order AO system are being carried out, as well as additional test characterizing the telescope and site. It is intended to continue such studies in an attempt to establish long term variances.

  15. Août/Magnétique

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    Julia Teles

    2015-12-01

    Full Text Available Julia Teles é técnica em áudio pelo IAV e bacharela em Composição Eletroacústica pela UNESP, tendo concluído o curso em 2012. Na universidade, foi aluna de Flo Menezes e Alexandre Lunsqui, além de ter contato com diversos compositores visitantes, como Annette Vande-Gorne, Barry Truax, entre outros. Em 2011 passou a integrar o grupo NME, atuando na área de produção e composição de obras para concertos, tendo estreado as obras Espasmo, Bezimiani, Miração; chá de coca, Transposição: dois pontos e Começo-fim em concertos do grupo em diversos espaços culturais do estado. Em 2009 iniciou seu estudo do theremin, instrumento com o qual realiza trilhas ao vivo, performances, improvisações, entre outras coisas. Em 2014 cursou composição acusmática sobre suporte analógico em um curso intensivo ministrado por Annette Vande-Gorne no Musiques et Recherches, na Bélgica. Atualmente faz parte do coletivo teatral 28 Patas Furiosas, situado em São Paulo, tendo criado a trilha sonora e operado o som do primeiro espetáculo do grupo, lenz, um outro. Há cerca de três anos atua como editora de som autônoma, tendo trabalhado principalmente no cinema (14 longas-metragens, realizando foleys e edição de som como assistente do sound designer Edson Secco. (Foto/Crédito: Beatriz Moura.

  16. Sensibilidade de estirpes de Bradyrhizobium ao glyphosate

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    Rodrigo Josemar Seminoti Jacques

    2010-02-01

    Full Text Available A aplicação do glyphosate sobre a soja resistente a este herbicida pode causar prejuízos à simbiose com o rizóbio. O objetivo deste trabalho foi avaliar a sensibilidade ao herbicida glyphosate de três estirpes de Bradyrhizobium recomendadas para a produção de inoculantes de sementes de soja no Brasil. Avaliou-se o efeito das concentrações de 0,0; 5,4; 10,8; 21,6 e 43,2 µg L-1 do ingrediente ativo do glyphosate [N-(fosfonometil glicina] no meio YM líquido sobre o crescimento de B. japonicum (estirpe SEMIA 5079 e de B. elkanii (estirpe SEMIA 5019 e estirpe SEMIA 587, por meio de leituras das densidades óticas e geração de curvas de crescimento. As reduções de crescimento na presença da menor concentração do glyphosate foram de 18% para SEMIA 5079, 29% para SEMIA 5019 e de 35% para SEMIA 587, sendo, de modo geral, quanto maior a concentração do herbicida no meio de cultura maior a inibição do crescimen­to. As estirpes apresentaram sensibilidade diferencial somente às concentrações mais baixas do glyphosate; nesse caso, foi possível determinar a seguinte ordem de sensibilidade: SEMIA 587 > SEMIA 5019 > SEMIA 5079. Essa sensibilidade diferencial é dependente da concentração do herbicida, pois na presença de 43,2 µg L-1 todas as estirpes tiveram seu crescimento severamente reduzido, não havendo diferença entre elas.

  17. A role for the retinoblastoma protein as a regulator of mouse osteoblast cell adhesion: implications for osteogenesis and osteosarcoma formation.

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    Bernadette Sosa-García

    Full Text Available The retinoblastoma protein (pRb is a cell cycle regulator inactivated in most human cancers. Loss of pRb function results from mutations in the gene coding for pRb or for any of its upstream regulators. Although pRb is predominantly known as a cell cycle repressor, our data point to additional pRb functions in cell adhesion. Our data show that pRb regulates the expression of a wide repertoire of cell adhesion genes and regulates the assembly of the adherens junctions required for cell adhesion. We conducted our studies in osteoblasts, which depend on both pRb and on cell-to-cell contacts for their differentiation and function. We generated knockout mice in which the RB gene was excised specifically in osteoblasts using the cre-lox P system and found that osteoblasts from pRb knockout mice did not assemble adherens junction at their membranes. pRb depletion in wild type osteoblasts using RNAi also disrupted adherens junctions. Microarrays comparing pRb-expressing and pRb-deficient osteoblasts showed that pRb controls the expression of a number of cell adhesion genes, including cadherins. Furthermore, pRb knockout mice showed bone abnormalities consistent with osteoblast adhesion defects. We also found that pRb controls the function of merlin, a well-known regulator of adherens junction assembly, by repressing Rac1 and its effector Pak1. Using qRT-PCR, immunoblots, co-immunoprecipitation assays, and immunofluorescent labeling, we observed that pRb loss resulted in Rac1 and Pak1 overexpression concomitant with merlin inactivation by Pak1, merlin detachment from the membrane, and adherens junction loss. Our data support a pRb function in cell adhesion while elucidating the mechanism for this function. Our work suggests that in some tumor types pRb inactivation results in both a loss of cell cycle control that promotes initial tumor growth as well as in a loss of cell-to-cell contacts, which contributes to later stages of metastasis.

  18. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jong-Kook [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Henry, Jon C. [Department of Surgery, Ohio State University, Columbus, OH 43210 (United States); Jiang, Jinmai [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Esau, Christine [Regulus Therapeutics, Carlsbad, CA (United States); Gusev, Yuriy [Lombardi Cancer Center, Georgetown University, Washington, DC (United States); Lerner, Megan R. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Postier, Russell G. [Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK (United States); Brackett, Daniel J. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Schmittgen, Thomas D., E-mail: Schmittgen.2@osu.edu [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States)

    2011-03-25

    Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.

  19. EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    Science.gov (United States)

    Mitra, Moutushy; Kandalam, Mallikarjuna; Harilal, Anju; Verma, Rama Shenkar; Krishnan, Uma Maheswari; Swaminathan, Sethuraman

    2012-01-01

    Purpose The molecular markers cluster of differentiation (CD)24, CD44, adenosine tri-phosphate (ATP) binding cassette protein G2 (ABCG2), and epithelial cell adhesion molecule (EpCAM) are widely used, individually or in combination, to characterize some types of cancer stem cells. In this study we characterized the EpCAM+ retinoblastoma (RB) cells for their cancer stem-like properties in vitro. Additionally, we targeted RB tumor cells via redirecting T cells using bispecific EpCAM×CD3 antibody. Methods Flow cytometry was used to study the co-expression of EpCAM with putative cancer stem cell markers, such as CD44, CD24, and ABCG2, in RB primary tumors. In vitro methyl thiazol tetrazolium (MTT) assay, invasion assay, and neurosphere formation assay were performed to characterize EpCAM+ cells for their cancer stem/progenitor cell-like properties. We assessed the in vitro efficacy of bispecific EpCAM×CD3 antibody on RB tumor cell proliferation and validated the results by evaluating effector cytokine production in the culture medium with the ELISA method. Results EpCAM was co-expressed with all cancer stem cell markers (CD44, CD24, and ABCG2) in primary RB tumors. EpCAM+ cells showed significantly higher proliferative invasive potential and neurosphere formation in vitro compared to EpCAM– Y79 cells. EpCAM+ cells showed higher β-catenin expression compared to EpCAMˉ cells. EpCAM×CD3 significantly retarded proliferation of RB primary tumor cells. EpCAM×CD3 effectively induced the secretion of effector cytokines, such as interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-10, IL-2, and transforming growth factor (TGF)-β1, and also perforin levels by pre-activated lymphocytes. Conclusions EpCAM might be a novel cancer stem cell marker in RB. EpCAM×CD3 antibody redirecting T cells to attack RB tumor cells may prove effective in RB management. Further preclinical studies are needed to confirm the initial findings of our study. PMID:22328825

  20. Retinoblastoma treatment: impact of the glycolytic inhibitor 2-deoxy-d-glucose on molecular genomics expression in LHBETATAG retinal tumors

    Directory of Open Access Journals (Sweden)

    Piña Y

    2012-05-01

    involved in its in vitro and in vivo activity in inhibiting tumor cell growth.Keywords: retinoblastoma, hypoxia, genetic expression, glycolytic inhibitor, 2-DG