WorldWideScience

Sample records for apagomerella versicolor coleoptera

  1. Mycological study of tinea versicolor

    Directory of Open Access Journals (Sweden)

    Sankara Rao I

    1997-01-01

    Full Text Available Mycological study of 100 cases of tinea versicolor was undertaken. Skin scrapings from 100 cases were subjected for culture in Sabouraud′s dextrose agar, out of which 60 positive cultures (60% were obtained.

  2. Blaschkoid pityriasis versicolor.

    Science.gov (United States)

    Tan, Cheng; Zhu, Wen-Yuan; Min, Zhong-Sheng

    2010-07-01

    A 31-year-old male patient complained of having follicular and brownish red maculopapules along the Blaschko's lines on the right chest for 2 days. On examination, follicular brownish maculopapules were present on the chest with a uniform size of about 3 mm in diameter. The lesions were isolated without a tendency to merge, giving several S-shaped, band-like appearances. Direct mycological examination of the skin flakes revealed many pseudomycelial hyphae and yeast cells with typical spaghetti and meatball appearance. Wood's light examination of the lesion revealed a golden yellow fluorescence. A diagnosis of blaschkoid pityriasis versicolor was suggested because of blaschkoid distribution of the lesions in this new variant of PV.

  3. Chemical constituents of Simarouba versicolor

    International Nuclear Information System (INIS)

    Arriaga, Angela M.C.; Mesquita, Aldenor C. de; Pouliquen, Yvone B.M.; Lima, Roberto A. de; Cavalcante, Sergio H.; Carvalho, Mario G. de; Siqueira, Jose A. de; Alegrio, Leila V.; Braz-Filho, Raimundo

    2002-01-01

    From the roots, stems and fruits of Simarouba versicolor (Simaroubaceae) were isolated quassinoids (3, 5-7), triterpenoids (8-14), a mixture of steroids (15-17), the flavonoid kaempferol (18) and the squalene derivative 11,14-diacetoxy-7,10; 15,18-diepoxy-6,19-dihidroxy-6,7,10,11,14,15,18,19-octahydrosqualene (19). Spectral data were used for structural characterization. (author)

  4. Chemical constituents of Simarouba versicolor

    Energy Technology Data Exchange (ETDEWEB)

    Arriaga, Angela M.C.; Mesquita, Aldenor C. de; Pouliquen, Yvone B.M. [Ceara Univ., Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica; Lima, Roberto A. de; Cavalcante, Sergio H. [Alagoas Univ., Maceio, AL (Brazil). Dept. de Quimica; Carvalho, Mario G. de; Siqueira, Jose A. de; Alegrio, Leila V. [Universidade Federal Rural do Rio de Janeiro, RJ (Brazil). Dept. de Quimica; Braz-Filho, Raimundo [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacases, RJ (Brazil). Setor de Quimica de Produtos Naturais]. E-mail: braz@uenf.br

    2002-09-01

    From the roots, stems and fruits of Simarouba versicolor (Simaroubaceae) were isolated quassinoids (3, 5-7), triterpenoids (8-14), a mixture of steroids (15-17), the flavonoid kaempferol (18) and the squalene derivative 11,14-diacetoxy-7,10; 15,18-diepoxy-6,19-dihidroxy-6,7,10,11,14,15,18,19-octahydrosqualene (19). Spectral data were used for structural characterization. (author)

  5. Oral fluconazole in tinea versicolor

    Directory of Open Access Journals (Sweden)

    Sankara Rao I

    1997-01-01

    Full Text Available 25 patients with extensive tinea versicolor were treated with single oral dose of 400 mg of fluconazole. 25 patients returned for follow-up. Follow-up at 3 weeks, 6 weeks and 8 weeks showed 100% clinical cure rate and 92% mycological cure rate. No significant side effects were noticed. The majority of patients found the treatment effective, safe and convenient.

  6. Triterpenoid saponins from Dianthus versicolor.

    Science.gov (United States)

    Ma, Li; Gu, Yu-Cheng; Luo, Jian-Guang; Wang, Jun-Song; Huang, Xue-Feng; Kong, Ling-Yi

    2009-04-01

    Seven new pentacyclic triterpenoid saponins, named dianversicosides A-G (1-7), together with nine known compounds, were isolated from the aerial parts of Dianthus versicolor. The structures of 1-7 were elucidated on the basis of spectroscopic data and chemical evidence. The absolute configuration of the 3-hydroxyl-3-methylglutaryl (HMG) group in 1-4 was ascertained by chemical analysis combined with a chiral HPLC method. Cytotoxic activities of the isolated compounds were evaluated against a small panel of cancer and other cell lines.

  7. Malassezia Species and Pityriasis Versicolor

    Directory of Open Access Journals (Sweden)

    Gulin Rodoplu

    2016-01-01

    Full Text Available Malassezia species are found in part of the normal human cutaneous commensal flora, however it has been known for many years that the Malassezia yeasts are associated with a number of different human diseases ranging from pityriasis versicolor to seborrhoeic dermatitis. In addition, since the 1980s, they have been reported as causing opportunistic systemic infections. The taxonomy of Malassezia spp. has recently been modified to include 13 obligatorily lipophilic species, plus one non-obligatorily lipophilic species, which only rarely colonizes human hosts and currently the genus consist 14 species as M. furfur, M. pachydermatis, M. sympodialis, M. globosa, M. obtusa, M. slooffiae, M. restricta, M. dermatis, M. japonica, M. nana, M. yamatoensis, M. caprae, M. equina, M. cuniculi. Fastidious growth requirements of Malassezia yeasts defied the initial attempts to culture these organisms and their true identification and the relationship between different species only became apparent with the application of modern molecular techniques. The causative fungus is seen especially in such seborrheic areas as the scalp, face, trunk and upper back. Under the influence of various exogenous or endogenous predisposing factors, these yeasts change from the blastospore form to the mycelial form and become pathogenic. Diagnosis of pityriasis versicolor which is caused by Malassezia species is generally easy and lies on the basis of its clinical appearance and can be confirmed by mycological examination. The diagnosisis is mainly based on direct examination with potassium hydroxide (KOH and demonstration that represents pseudohyphae and blastoconidia as the typical %u201Cspaghetti and meatballs%u201D pattern. Characteristic features of the genus Malassezia include a distinctive morphology and an affinity for lipids in culture. Culture is necessary to recover the infecting strain, especially for epidemiologic purposes and also to test its antifungal susceptibility

  8. Atypical pityriasis versicolor case report

    Directory of Open Access Journals (Sweden)

    Zonunsanga

    2015-04-01

    Full Text Available Pityriasis versicolor is a superficial fungal infection caused by mycelial form of Malassezia spp, which is confined to stratum corneum. It usually present in the trunk as either hypo or hyperpigmented, aymptomatic, round to oval macules of varying sizes, which may merged to form geographic shape. Diagnosis is usually done clinically, or KOH examination which shows typical spagetti and meat balls appearances, or even by wood’s lamp which shows orange to yellow fluorescence. The case series had been recording in between 2012 to 2013. Within that period, we had recorded 32 cases. All the patients which we had recorded presented with multiple, asymptomatic macules of small sizes varying from 1-2 cm in diameter to 3-4mm in diameter, usually round to oval, hypopigmented, non scaly lesions. 26 patients had lesions on forearms, 3 patients had lesions on dorsa of hands bilaterally, 3 patients had similar kind of lesions on thigh. Besnier’s test was positive in 14 (43.75% patients. KOH examinations showed fungal hyphae in 14 (33.33% patients with typical spagetti and meat balls appearances in 9 (8.13% patients. All of them were given and all of them got response and healed within 2-4 months.

  9. Fluconazole and itraconazole in pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Jaswal Ritu

    1999-01-01

    Full Text Available Pityriasis versicolor is a common superficial fungal infection caused by Malassezia species. It has a high incidence and prevalence in tropical climates. Although it responds well to treatment, relapses and recurrences are frequent. In the present study the therapeutic response of single dose fluconazole (400 mg with itraconazole (100mg twice daily ? 7 days was compared in sixty patients of pityriasis versicolor. No significant statistical difference (p>0.05% was observed between efficacy of two drugs. Therapy with fluconazole is preferable in view of single dose administration and lesser cost as compared to itraconazole.

  10. Steroids isolated from Millettia versicolor Baker (Fabaceae)

    African Journals Online (AJOL)

    STORAGESEVER

    2008-06-03

    Jun 3, 2008 ... African Journal of Biotechnology Vol. 7 (11), pp. ... Full Length Research Paper ... of Millettia versicolor Baker, a medicinal plant used in the traditional ... The spectral analysis enabled us to identify 4 known .... evidence for participation of protein kinase C and protein kinase A ... Neoplasma 51(6): 407-414.

  11. PITYRIASIS VERSICOLOR – POSSIBLE GENETIC BASIS ...

    African Journals Online (AJOL)

    Non-occurrence of Pityriasis versicolor (PV) in spouses of individuals with this superficial fungal infection despite several years of cohabitation suggests that heredity might play an important role in those affected. Forty subjects who were married were studied in two phases. The first phase involved using a detailed ...

  12. BIOTECHNOLOGICAL APPLICATIONS AND POTENTIAL USES OF THE MUSHROOM TRAMESTES VERSICOLOR

    OpenAIRE

    CÓRDOBA M., Ketty A.; RÍOS H., Alicia

    2012-01-01

    The use of products obtained from fungi (particularly mushrooms) has increased lately due to their broad applicability in different scientific and industrial fields. The genus Trametes comprises a group of white rot producing ligninolytic fungi, with medicinal properties, biotechnological importance and environmental applications. One of the most potentially useful species is T. versicolor, formerly known as Coriolus versicolor or Polyporus versicolor. Also known as Yun Zhi in China, is a fun...

  13. Micosis superficiales: Candidiasis y pitiriasis versicolor

    Directory of Open Access Journals (Sweden)

    Rubén José Larrondo Muguercia

    2001-12-01

    Full Text Available Se exponen las características clínicas de las lesiones cutáneas en las candidiasis y la pitiriasis versicolor. Se hace hincapié en las medidas de educación, prevención y control de estas afecciones a nivel primario de atención médica. Se exponen además las diferentes maniobras terapéuticas, tanto tópicas como sistémicas, con las que se cuenta para su tratamiento, y se hace referencia a las medidas terapéuticas alternativas que brinda la medicina natural y tradicional para estas afecciones.The clinical characteristics of the skin lesions in candidiasis and pityriasis versicolor are dealth with. Emphasis is made on the measures of education, prevention and control of these affections at the primary health care level. The different topic and systematic therapeutic maneuvers to be used in the treatment are explained. Reference is made to the alternative therapeutic measures offered by traditional and natural medicine for these affections.

  14. Pityriasis versicolor in primary school children in Medan Labuhan

    Science.gov (United States)

    Febriyanti; Paramita, DA; Eyanoer, P.

    2018-03-01

    Personal hygiene is an activity in which done by someone, directly and indirectly, to keep healthy to prevent the risk of diseases. Personal hygiene is also a factor that plays a role in the occurrence of skin diseases and one of them is Pityriasis versicolor. Pityriasis versicolor is a superficial fungal infection caused by Malassezia furfur which is a saprophytic organism in the skin. Activation of M. furfur into a pathogenic form due to changes in the normal flora of the skin that caused by several factors and one of them is poor personal hygiene. A cross-sectional study consist of 115 eligible students is done to determine the relationship between personal hygiene with the occurrence of Pityriasis versicolor among students in primary school in Medan Labuhan district. The result showed that 59.8% of the subject with poor personal hygiene and there is a significant association between personal hygiene with Pityriasis versicolor (p<0.05).

  15. Ketoconazole 2% shampoo in pityriasis versicolor: An open trial

    Directory of Open Access Journals (Sweden)

    Rathi S

    2003-03-01

    Full Text Available Pityriasis versicolor is a common superficial fungal infection caused by a lipophilic yeast. The aim of the study was to evaluate the efficacy of ketoconazole 2% shampoo in the treatment of pityriasis versicolor, for which thirty patients were included. The shampoo was applied daily for 3 days and found to be very effective in clearing the signs and symptoms of the disease. There was no serious adverse effects.

  16. Pityriasis versicolor in the pediatric age group

    Directory of Open Access Journals (Sweden)

    Jena Deepak

    2005-01-01

    Full Text Available BACKGROUND: Pityriasis versicolor (PV is a mild chronic infection of the skin caused by Malassezia yeasts. Although it is primarily seen in adults, children are often affected in the tropics . METHODS: Over a period of 2 years, children (up to the age of 14 years who were clinically and mycologically diagnosed as PV were included in the study. The clinical and epidemiological pattern in different age groups was noted. RESULTS: PV in this age group formed about 31% of the total cases of PV; 4.8% cases presented in infancy. The commonest site of involvement was the face in 39.9% of the cases. Most of the cases presented in summer months. CONCLUSIONS: PV is not an uncommon disease among children in the tropics. There is a sudden resurgence of cases in the hot monsoons and even infants are not spared.

  17. Fluconazole in the therapy of Pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Poljački Mirjana N.

    2005-01-01

    Full Text Available The authors present the results of the systemic application of flukonazole in therapy of Pityriasis versicolor. It was arranged for the total number of 38 patients, 18 females and 20 males. The diagnosis of diseases was established on the base of the clinical examination, the native mycological examination and by the using of Wood lamps. The therapy was passed by the using of 300 mg flukonazole in a single dose, once weekly, during two weeks. The following period amounted to one week after the passed therapy. The therapeutic efficacy was assessed with regard to the clinical and mycological healing. The clinical efficacy was assessed semiquantitative on the base of increasing of the percentage rates of the total score of disease that was computed by collecting of the numeric values for every clinical argument typical for the disease, and the mycological efficacy on the base of the mycological findings and the fluorescence finding after lightening with the Wood's lamp. The controlling examinations were performed on day 0, 14 and 22. The results of investigations have shown that the complete clinical healing was achieved after two weeks of therapy in 94,74%, and the mycological healing in 92,11% patients. The rate of the mycological healing was evaluated after 1 week of following period equal to the rate of the clinical healing and it was also 94,74%. The undesirable effects of the drug applications weren't by any patient.

  18. Acral pityriasis versicolor – A rare clinical presentation

    Directory of Open Access Journals (Sweden)

    Tasleem Arif

    2015-04-01

    Full Text Available Pityriasis versicolor is a superficial fungal infection of the skin caused by the yeast of the genus Malassezia and presents as hypo or hyper pigmented scaly macules. The most commonly affected sites include upper trunk, upper arms, neck and the abdomen. Lesions confined to the acral parts like hands and feet have rarely been reported. In this article the author reports a 40 year old male who presented with multiple hypo pigmented scaly macules confined to the acral parts (hands and wrist. The acral variant of pityriasis versicolor is considered to be a very rare clinical entity which prompted the author to report this case.

  19. Clinicomycological profile of pityriasis versicolor in Assam.

    Science.gov (United States)

    Sharma, Ajanta; Rabha, Debajit; Choraria, Saveta; Hazarika, Debeeka; Ahmed, Giasuddin; Hazarika, Naba Kumar

    2016-01-01

    Geographical variation in the distribution of Malassezia species associated with pityriasis versicolor (PV) has led to the necessity of studying epidemiological, mycological, and clinical characteristics of PV. To study the epidemiological, mycological, and clinical characteristics of PV in a tertiary care hospital. The study was carried out with a cross-sectional design. Two hundred and sixty-two consecutive PV patients were subjected to detailed history, clinical examination, and investigations. Skin scrapings were processed by direct microscopy and culture. Isolates were identified by phenotypic characteristics and polymerase chain reaction-restriction fragment length polymorphism. Association of Malassezia species with clinical and epidemiological characteristics was studied. Statistical analysis of the data was done using statistical software. Maximum number of PV cases (33.9%) belonged to the age group of 21-30 years with a male preponderance. 61.4% of the patients had a sedentary lifestyle, 70.2% showed the gradual onset of the disease, 51.1% presented with pruritus and in 66.4% of the patients symptoms were continuous. Most commonly involved body site was neck (27.8%), 77.09% of the lesions were bilaterally asymmetrical, 87.4% were macular, and 89.3% were hypopigmented. Malassezia furfur (77.3%) was the predominant species. Sedentary lifestyle (61.4%) and increased sweating (48%) were the most commonly associated predisposing factors. PV is more common in males. Distribution of Malassezia species varies significantly from those reported in other parts of India. M. furfur was the most common species responsible for PV in our region. Hence, further studies are required to evaluate the exact cause of this variation.

  20. Clinicomycological profile of pityriasis versicolor in Assam

    Directory of Open Access Journals (Sweden)

    Ajanta Sharma

    2016-01-01

    Full Text Available Background: Geographical variation in the distribution of Malassezia species associated with pityriasis versicolor (PV has led to the necessity of studying epidemiological, mycological, and clinical characteristics of PV. Aims: To study the epidemiological, mycological, and clinical characteristics of PV in a tertiary care hospital. Settings and Design: The study was carried out with a cross-sectional design. Materials and Methods: Two hundred and sixty-two consecutive PV patients were subjected to detailed history, clinical examination, and investigations. Skin scrapings were processed by direct microscopy and culture. Isolates were identified by phenotypic characteristics and polymerase chain reaction-restriction fragment length polymorphism. Association of Malassezia species with clinical and epidemiological characteristics was studied. Statistical analysis of the data was done using statistical software. Results: Maximum number of PV cases (33.9% belonged to the age group of 21–30 years with a male preponderance. 61.4% of the patients had a sedentary lifestyle, 70.2% showed the gradual onset of the disease, 51.1% presented with pruritus and in 66.4% of the patients symptoms were continuous. Most commonly involved body site was neck (27.8%, 77.09% of the lesions were bilaterally asymmetrical, 87.4% were macular, and 89.3% were hypopigmented. Malassezia furfur (77.3% was the predominant species. Sedentary lifestyle (61.4% and increased sweating (48% were the most commonly associated predisposing factors. Conclusion: PV is more common in males. Distribution of Malassezia species varies significantly from those reported in other parts of India. M. furfur was the most common species responsible for PV in our region. Hence, further studies are required to evaluate the exact cause of this variation.

  1. Activity enhancement of ligninolytic enzymes of Trametes versicolor ...

    African Journals Online (AJOL)

    Suspended cultures of white-rot fungus, Trametes versicolor, supplemented with bagasse powder showed a concentration dependent enhancement in the ligninolytic enzymes activity in liquid shake cultures. 2% (w/v) bagasse powder improved greater stability to the enzymes. The optimum pH is 3.5 and the optimum ...

  2. The effect of surfactant on pollutant biosorption of Trametes versicolor

    Science.gov (United States)

    Gül, Ülküye Dudu; Silah, Hülya; Akbaş, Halide; Has, Merve

    2016-04-01

    The major problem concerning industrial wastewater is treatment of dye and heavy metal containing effluents. Industrial effluents are also contained surfactants that are used as levelling, dispersing and wetting agents. The purpose of this study was to investigate the effect of surfactant on textile dye biosorption properties of a white rot fungus named Trametes versicolor. Reactive dyes are commonly used in textile industry because of their advantages such as brightness and excellent color fastness. A recative textile dye, called Everzol Black, was used in this study. The low-cost mollasses medium is used for fungal growth. The usage of mollases, the sugar refinery effluent as a source of energy and nutrients, gained importance because of reducing the cost and also reusing another waste. In biosorption process the effect of surfactant on dye removal properties of T. versicolor was examined as a function of pH, dye consentration and surfactant concentration. The results of this study showed that the surfactant enhanced the dye removal capacity of Trametes versicolor. The dye and surfactant molecules were interacted electrostatically and these electrostatic interactions improved dye removal properties of filamentous fungus T. versicolor. The results of this study recommended the use of surfactants as an inducer in textile wastewater treatment technologies.

  3. Toxicity effects of ethanol extract of Simarouba versicolor on ...

    African Journals Online (AJOL)

    usuario

    2016-02-24

    Feb 24, 2016 ... versicolor (Sv-EtOH) on the reproductive system of rats were investigated; also the influence of ... The following constituted the chemicals and drugs used in this ... dilution vehicle, n = number of animals; o.a = oral admnistration; im = intramuscular. .... Birth delivery, birth rates and viability were determined.

  4. Cytotoxic activities of Coriolus versicolor (Yunzhi) extracts on human ...

    African Journals Online (AJOL)

    SERVER

    2007-08-06

    Aug 6, 2007 ... four human liver cancer (7703, HepG2, 7721, PLC) and four human breast cancer (Bcap37, ZR75-30,. MCF-7, T-47D) cell lines ... Key words: Coriolus versicolor, fruit body, polysaccharide, anti-tumor. INTRODUCTION. Coriolus ... somewhat in structure, composition, and physiological activity. The present ...

  5. A study on the antioxidant effect of Coriolus versicolor ...

    African Journals Online (AJOL)

    SOD, MDA and GSH-Px levels in rat brain tissues were determined with SD rats as the animal model. The results showed that Coriolus versicolor polysaccharide can reduce the lipid peroxidation level in brain tissues during exhaustive exercise in rats, and can accelerate the removal of free radicals. The study concluded ...

  6. Biological Control of Fusarium oxysporum f.sp. cumini with Aspergillus versicolor

    Directory of Open Access Journals (Sweden)

    S. Israel

    2005-04-01

    Full Text Available A native heat-tolerant strain of Aspergillus versicolor (Vuill. Tirab. highly antagonistic to Fusarium oxysporum f. sp. cumini (Foc was isolated from arid soils. In tests performed to ascertain its antagonistic activity against Foc as compared to Trichoderma harzianum, a 99.2 and 96.4% reduction in Foc propagules was achieved in A. versicolor and T. harzianum infested soil respectively. The reduction of Foc propagules in Foc and A. versicolorinfested soil was also determined. In a liquid-culture test, even at a low concentration of 0.5 ml cell-free filtrate, A. versicolor inhibited mycelial growth of Foc. Population changes of A. versicolor were examined at different soil moisture gradients, where maximum survival and multiplication of A. versicolor was estimated at 50% of moisture holding capacity. In general, with increasing concentrations of A. versicolor inoculum, soil population densities of Foc went down. Studies on thermal resistance showed that A. versicolor survived and multiplied even at 65°C. Soil amended with A. versicolor alone, or with a combination of T. harzianum and Verbisina enceloides residues was significantly better at reducing Foc than was non-amended control soil. A marked increase in the root length of cumin was observed in soil amended with A. versicolor or T. harzianum or both. The results suggest that A. versicolor has a potential value for use against Fusarium in hot arid soils because it can survive under dry and high-temperature conditions.

  7. Novos aspectos na evolução clínica da pitiríase versicolor New aspects in the clinical course of pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Valéria Maria de Souza Framil

    2011-12-01

    Full Text Available FUNDAMENTO: A pitiríase versicolor é uma doença infecciosa causada por várias espécies de Malassezia com uma tendência a se tornar recidivante ou crônica. OBJETIVOS: Este trabalho foi conduzido na tentativa de conhecer a evolução clínica da pitiríase versicolor em relação ao número de recidivas após um tratamento adequado no período de 12 meses e correlacionar o número de recidivas com as espécies de Malassezia isoladas. MATERIAL E MÉTODOS: Cento e dois pacientes com diagnóstico clínico e laboratorial de pitiríase versicolor foram acompanhados por um período de 12 meses para observarmos o número de recidivas da doença. RESULTADOS: A pitiríase versicolor, após um tratamento adequado, apresentou três tipos de evolução clínica num período de 12 meses: pitiríase versicolor sem nenhum episódio de recidiva (32,35%; pitiríase versicolor recidivante, com um a quatro episódios de recidiva (52,94% devidos a fatores de predisposição relacionados; e pitiríase versicolor crônica, com mais de quatro episódios de recidiva (14,70% sem nenhuma relação com fatores de predisposição. CONCLUSÕES: A pitiríase versicolor apresentou uma evolução clínica de acordo com o número de episódios de recidiva da doença analisados durante um período de 12 meses que pode ser considerada da seguinte maneira: pitiríase versicolor com cura clínica e micológica, pitiríase versicolor recidivante e pitiríase versicolor crônica.BACKGROUND: Pytiriasis versicolor is an infectious disease caused by several Malassezia species which has a tendency to become relapsing or chronic. OBJECTIVES: This study was conducted in an attempt to investigate the clinical course of pityriasis versicolor with regard to the number of relapses after a 12-month therapy and correlate this number with isolates of Malassezia species. MATERIAL AND METHODS: 102 patients with clinical and laboratory diagnosis of pityriasis versicolor were monitored for 12

  8. Comparative histopathology of Pityriasis versicolor and Pityrosporum folliculitis

    Directory of Open Access Journals (Sweden)

    Mittal R

    1992-01-01

    Full Text Available Twenty five cases each of pityriasis versicolor (PV and pityrosporum folliculitis (PF were selected for this study. Histopathologically in PV, spores and hyphae in spaghetti and meatball appearance in stratum corneum, and perivascular mononuclear infiltrate were seen. In PF, spores were inside the dilated hair follicles filled with keratin, perifollicular and perivascular mononuclear infiltration were seen. Basket weave hyperkeratosis, edema and disruption of collagen were seen in 88% cases of PF and have not been reported earlier.

  9. Clinical Trial Of Ketoconazole 2% + Zinc Pyrithione 1% Shampoo In The Management Of Tinea Versicolor

    Directory of Open Access Journals (Sweden)

    Ramesh M

    2004-01-01

    Full Text Available An open uncontrolled trial, to assess the efficacy of ketoconazole 2%+zinc pyrithione 1% shampoo was conducted in patients with extensive tinea versicolor. The results show that this is an effective and well tolerated combination in the management of tinea versicolor.

  10. Spiny lobster Panulirus versicolor filogenetic and genetic in Lombok waters, West Nusa Tenggara, Indonesia

    Directory of Open Access Journals (Sweden)

    Pranata B.

    2018-02-01

    Full Text Available This study aims to identify the phylogenetic spiny lobster Panulirus versicolor in Lombok waters, Indonesia and its association with P. versicolor spiny lobster from several regions of the Indian Ocean based on the cytochrome oxidase I (COI gene. The researchers collected tissue samples from 13 P. versicolor spiny lobster in Lombok waters. 9 haplotypes were identified with haplotype diversity values (Hd and nucleotides (Pi respectively Hd = 0.859 and Pi = 0.00509. Research results exhibit P. versicolor spiny lobster population from the waters of Lombok is closely related to the spiny lobster population in some regions of the Indian Ocean. In general, P. versicolor spiny lobster population formed a monophyletic clone with spiny lobsters from several regions of the Indian Ocean with genetic distance values (P-distance from 0.001 to 0.004. The reconstruction of the haplotype network exhibited no genetic structure, which means that each population is not genetically isolated from others.

  11. Tinea versicolor, tinea nigra, white piedra, and black piedra.

    Science.gov (United States)

    Bonifaz, Alexandro; Gómez-Daza, Fernando; Paredes, Vanessa; Ponce, Rosa María

    2010-03-04

    Superficial mycoses are fungal infections limited to the stratum corneum and its adnexal structures. The most frequent types are dermatophytoses or tineas. Tinea versicolor involves the skin in the form of hypochromic or hyperchromic plaques, and tinea nigra affects the skin of the palms with dark plaques. White piedra and black piedra are parasitic infections of scalp hairs in the form of concretions caused by fungal growth. Diagnosis of these mycoses is made from mycologic studies, direct examination, stains, and isolation, and identification of the fungi. Treatment includes systemic antifungals, topical antifungals, and keratolytics. Copyright 2010 Elsevier Inc. All rights reserved.

  12. 1825 (Coleoptera: Cerambycidae

    Directory of Open Access Journals (Sweden)

    Márcia d´Avila

    2006-01-01

    Full Text Available The erva-mate (Ilex paraguariensis A.St.-Hil. has a social, cultural and economic importance in the southern states of Brazil. The pure stands of this culture was responsible for the increase of many species of insects. Hedypathes betulinus (Klug, 1825 (Coleoptera: Cerambycidae is considered the main pest from an economic viewpoint because of its difficult control and potential for damage. The larval phase occurs inside the twings and trunks, what makes more difficult to deal with its detention and management. Cultural and mechanical management are the most indicated, such as collection of adults, prunning and burning of plant parts damaged by the insect, balanced nutrition, adequate plant density and maintenance of areas with native vegetation or also the introduction of policulture. These strategies may increase the agroecossystem balance and thus a reduction of the insect-pest to an aceptable level. Therefore, the objective of this study was to assemble and the discuss the information on the bioecology and management of erva-mate borer.

  13. Study of oral Ketoconazole on recurrent & extensive Pityriasis Versicolor

    Directory of Open Access Journals (Sweden)

    Moghaddami M

    1997-04-01

    Full Text Available Several topical agents have been used for treating Pityriasis versicolor. But the main problem is recurrence. In this study 82 patients with recurrent and/or extensive from of infection were treated with oral Ketoconazole. 200 mg once daily for a maximum of 4 weeks. Seventeen patients have been infected for the first time and sixty five for the several times. The duration of disease in most cases (35.4% were between 1-3 years. Out of 82 studied patients only 60 cases were referred for follow up. The clinical and mycological cure were seen in 54 cases (90% after 15 days therapy. In other 6 patients after 15 days the lesions have not cured completely so the treatment was continued for other 2 weeks, but after that, only one of them referred to the clinic, which treatment extended with topical therapy.

  14. Pretreatment of radiata pine using two white rot fungal strains Stereum hirsutum and Trametes versicolor

    International Nuclear Information System (INIS)

    Shirkavand, Ehsan; Baroutian, Saeid; Gapes, Daniel J.; Young, Brent R.

    2017-01-01

    Highlights: • Fungal pretreatment by two New Zealand native white rot fungi was proposed. • Trametes versicolor was more efficient in selective degradation of pine wood chips. • Both fungal strains significantly decreased crystallinity index of biomass only after week 7 of degradation. • Structural analysis showed that Trametes versicolor and Stereum hirsutum increased porous surface area of woody biomass. - Abstract: Stereum hirsutum and Trametes versicolor, were studied over a period of 3–7 weeks for pretreatment of radiata pine wood chips. Chemical analysis of pretreated biomass showed that the two studied strains were able to selectively degrade lignin. Selective lignin degradation was greater in week 3 of the pretreatment by Trametes versicolor compared to the other strain. Lengthening pretreatment time increased both lignin and cellulose losses which caused a reduction in selective lignin degradation for both strains. X-ray diffractometry showed that after seven weeks of pretreatment, the crystallinity of the woody biomass was decreased significantly. It decreased from 46% for untreated wood chips to 37% and 44% for Stereum hirsutum and Trametes versicolor treated biomass, respectively. The pretreatment with these two white rot fungi showed that 3-week pretreatment provided a cellulose rich biomass with the minimum cellulose loss compared to the other time of pretreatment.

  15. Bioremediation of zirconium from aqueous solution by coriolus versicolor: process optimization

    International Nuclear Information System (INIS)

    Amin, M.; Bhatti, H. N.; Sadaf, S.

    2013-01-01

    In the present study the potential of live mycelia of Coriolus versicolor was explored for the removal of zirconium from simulated aqueous solution. Optimum experimental parameters for the bioremediation of zirconium using C. versicolor biomass have been investigated by studying the effect of mycelia dose, concentration of zirconium, contact time and temperature. The isothermal studies indicated that the ongoing bioremediation process was exothermic in nature and obeyed Langmuir adsorption isotherm model. The Gibbs free energy (ΔG), entropy (ΔS) and enthalpy (ΔH) of bioremediation were also determined. The result showed that bioremediation of zirconium by live C. versicolor was feasible and spontaneous at room temperature. The equilibrium data verified the involvement of chemisorption during the bioremediation. The kinetic data indicated the operation of pseudo-second order process during the biosorption of zirconium from aqueous solution. Maximum bioremediation capacity (110.75 mg/g) of C. versicolor was observed under optimum operational conditions: pH 4.5, biomass dose 0.05 mg/100 mL, contact time 6 h and temperature 30 degree C. The results showed that C. versicolor could be used for bioremediation of heavy metal ions from aqueous systems. (author)

  16. Direct human DNA protection by Coriolus versicolor (Yunzhi) extract.

    Science.gov (United States)

    Szeto, Yim Tong; Lau, Po Chun; Kalle, Wouter; Pak, Sok Cheon

    2013-07-01

    Scientific evidence has shown Coriolus versicolor (L. ex Fr.) Quel (also known as Yunzhi) has the role of immunomodulator in therapeutic effect. The aim of this in vitro study was to investigate the antioxidative effect of Yunzhi and to explore the mechanisms behind its DNA protection. Commercial Yunzhi extract was dissolved in water and diluted in five concentrations (10(1)-10(5) μg/L) with appropriate buffers. Lymphocytes harvested from three healthy subjects were incubated with Yunzhi extract for 30 min. Cells were then subjected to 5 min oxidant challenge by 45 μM hydrogen peroxide. The standard alkaline comet (SAC) assay and lysed cell comet (LCC) assay were performed in parallel. DNA damage of each treatment was scored under a fluorescence microscope and compared with the cells without Yunzhi pretreatment. U-shaped dose-response was seen in both versions of the comet assay. Yunzhi at 10(4) μg/L demonstrated a genoprotective effect against oxidative damage in the SAC assay (25% decrease in comet score). In the LCC assay, a trend of protection in lymphocytes was observed but it did not reach statistical significance. A direct antioxidant effect of Yunzhi against oxidant challenge on the DNA of lymphocytes was evidenced. The active component in Yunzhi was likely to be membrane permeable.

  17. Atrophic pityriasis versicolor occurring in a patient with Sjögren's syndrome.

    Science.gov (United States)

    Marinello, Elena; Piaserico, Stefano; Alaibac, Mauro

    2017-01-18

    Pityriasis versicolor is one of the most frequent epidermal mycotic infections in the world, but its atrophic variant is rarely described. The aetiology of the atrophy is still unknown, and two main hypotheses have been formulated, one suggesting a correlation with long-term use of topical steroids and the other a delayed type hypersensitivity to epicutaneous antigens derived from components of the fungus. Atrophic pityriasis versicolor is a benign disease, but needs to be distinguished from other more severe skin diseases manifesting with cutaneous atrophy. The diagnosis can be easily confirmed by direct microscopic observation of the scales soaked in 15% potassium hydroxide, which reveals the typical 'spaghetti and meatball' appearance, or by a skin biopsy in doubtful cases. Here, we describe a case of extensive atrophic pityriasis versicolor occurring in a woman affected by Sjögren's syndrome which completely resolved after topical antifungal treatment. 2017 BMJ Publishing Group Ltd.

  18. Effect of Aspergillus versicolor strain JASS1 on low density polyethylene degradation

    Science.gov (United States)

    Gajendiran, A.; Subramani, S.; Abraham, J.

    2017-11-01

    Low density polyethylene (LDPE) waste disposal remains one of the major environmental concerns faced by the world today. In past decades, major focus has been given to enhance the biodegradation of LDPE by microbial species. In this present study, Aspergillus versicolor with the ability to degrade LDPE was isolated from municipal landfill area using enrichment technique. Based on 18S rRNA gene sequencing confirmed its identity as Aspergillus versicolor. The biodegradation study was carried out for 90 d in M1 medium. The degradation behaviour of LDPE films by Aspergillus versicolor strain JASS1 were confirmed by weight loss, CO2 evolution, Scanning electron microscopy (SEM) analysis, Atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR) technique. From current investigation, it can be concluded that our isolated strain JASS1 had the potential to degrade LDPE films and it can be useful in solving the problem caused by polyethylene in the environment.

  19. Sampling methods for Graphoderus bilineatus (Coleoptera: Dytiscidae).

    NARCIS (Netherlands)

    Koese, B.; Cuppen, J.G.M.

    2006-01-01

    Onderzoek naar vangmethodes voor Graphoderus bilineatus (Coleoptera: Dytiscidae) Als onderdeel van een integrale studie naar de waterroofkever Graphoderus bilineatus in Nederland (in opdracht van het ministerie van lnv), werd een vergelijkend onderzoek uitgevoerd naar verschillende

  20. Pitiríase versicolor circinada: isolamento de Malassezia sympodialis - Relato de caso Pityriasis versicolor circinata: isolation of Malassezia sympodialis - Case report

    Directory of Open Access Journals (Sweden)

    Valéria Maria de Souza Framil

    2010-04-01

    Full Text Available Os autores descrevem caso de pitiríase versicolor circinada, cujo agente etiológico isolado foi Malassezia sympodialis em uma mulher de 34 anos. O isolamento e identificação da Malassezia sympodialis foi em ágar Dixon modificado e o método molecular para confirmação da espécie foi PCR-RFLP (polymerase chain reaction and restriction fragment length polymorphism analysis.The authors report a case of pityriasis versicolor circinata whose isolated etiologic agent was Malassezia sympodialis in a 34-year-old woman. The isolation and identification of Malassezia sympodialis were accomplished with modified Dixon's agar, and the molecular method used to confirm the species was polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP.

  1. Exploitation of Trametes versicolor for bioremediation of endocrine disrupting chemicals in bioreactors.

    Directory of Open Access Journals (Sweden)

    Cinzia Pezzella

    Full Text Available Endocrine disrupting chemicals (EDCs are environmental contaminants causing increasing concerns due to their toxicity, persistence and ubiquity. In the present study, degradative capabilities of Trametes versicolor, Pleurotus ostreatus and Phanerochaete chrysosporium to act on five EDCs, which represent different classes of chemicals (phenols, parabens and phthalate and were first applied as single compounds, were assessed. T. versicolor was selected due to its efficiency against target EDCs and its potentialities were exploited against a mixture of EDCs in a cost-effective bioremediation process. A fed-batch approach as well as a starvation strategy were applied in order to reduce the need for input of 'fresh' biomass, and avoid the requirement for external nutrients. The fungus was successfully operated in two different bioreactors over one week. Semi-batch cultures were carried out by daily adding a mixture of EDCs to the bioreactors in a total of five consecutive degradation cycles. T. versicolor was able to efficiently remove all compounds during each cycle converting up to 21 mg L-1 day-1 of the tested EDCs. The maintained ability of T. versicolor to remove EDCs without any additional nutrients represents the main outcome of this study, which enables to forecast its application in a water treatment process.

  2. Pitiríase versicolor e síndrome da imunodeficiência adquirida (SIDA

    Directory of Open Access Journals (Sweden)

    Roza Marlon Soares

    2003-01-01

    Full Text Available FUNDAMENTOS: A pitiríase versicolor é uma infecção crônica recorrente da camada córnea causada pela Malassezia furfur, geralmente assintomática, podendo ser causa de fungemia. A síndrome da imunodeficiência adquirida caracteriza-se por uma importante depressão celular, o que propicia a ocorrência de infecções oportunistas. OBJETIVOS: Dados a imunodepressão causada pelo HIV e o risco da presença de M. furfur em sua forma patogênica no imunocomprometido, realizou-se este trabalho comparativo buscando as reais diferenças da pitiríase versicolor no imunocompetente e no imunodeprimido pelo HIV. PACIENTES E MÉTODOS: Foram avaliados, no período de julho de 1998 a junho de 1999, 50 pacientes HIV positivos do Hospital Universitário da UFJF. Como grupo controle, foram examinados de forma aleatória 50 pacientes soronegativos para HIV. Para cada paciente foi preenchido um protocolo constando de identificação e exames clínicos e laboratoriais. RESULTADOS: A pitiríase versicolor foi diagnosticada clinicamente no grupo HIV positivo em sete (14% pacientes, resultado coincidente no grupo controle. Quanto ao passado para a pitiríase versicolor, 12 (24% pacientes já haviam apresentado tal doença no grupo HIV positivo, e igual incidência ocorreu no grupo controle. CONCLUSÕES: A pitiríase versicolor não se mostrou mais freqüente e nem mais agressiva na população imunodeprimida pelo HIV em relação ao grupo controle, enquanto a dermatite seborréica apresentou-se mais freqüente no grupo infectado pelo HIV.

  3. Identification and speciation of Malassezia in patients clinically suspected of having pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Avani Shah

    2013-01-01

    Full Text Available Background: Malassezia is a lipid-dependent yeast known to cause Pityriasis versicolor, a chronic, recurrent superficial infection of skin and present as hypopigmented or hyperpigmented lesions on areas of skin. If not diagnosed and treated, it may lead to disfigurement of the areas involved and also result in deep invasive infections. Aim: The aim of the present study was to identify and speciate Malassezia in patients clinically suspected of having Pityriasis versicolor. Materials and Methods: Total 139 patients suspected of having Pityriasis versicolor were evaluated clinically and diagnosis was done by Wood′s lamp examination, confirmed mycologically by using KOH, cultivation on Sabouraud′s dextrose agar and modified Dixon agar at a tertiary care hospital in Mumbai. The total duration of study was 12 months. Results: Majority of the patients were males (59.71% in the age group of 21-30 years (33.81% who were students (30.21% by profession. The incidence of Malassezia in Pityriasis versicolor was 50.35%. The most common isolate was M. globosa (48.57%, followed by M. furfur (34.28%. Majority of the patients had hypopigmented lesions, with M. globosa as the predominant isolate. Neck was the most common site affected; 88.48% were Wood′s lamp positive of which 56.91% of Malassezia isolates grew on culture. KOH mount was positive in 82.01% of which 61.40% Malassezia isolates grew on culture. Conclusions: The procedure of culture and antifungal testing is required to be performed as different species of Malassezia are involved in Pityriasis versicolor and susceptibility is different among different species. Thus, it would help to prevent recurrences and any systemic complications.

  4. DESTRUCTION OF ASPERGILLUS VERSICOLOR, PENICILLIUM CRYSOGENUM, STACHYBOTRYS CHARTARUM, AND CLADOSPORIUM CLADOSPORIDES SPORES USING CHEMICAL OXIDATION TREATMENT PROCESS

    Science.gov (United States)

    The survival of aqueous suspensions of Penicillium chrysogenum, Stachybotrys chartarum, Aspergillus versicolor, and Cladosporium cladosporioides spores was evaluated using various combinations of hydrogen peroxide and iron (II) as catalyst. Spores were suspended in water and trea...

  5. Biodegradation and utilization of 4-n-nonylphenol by Aspergillus versicolor as a sole carbon and energy source

    International Nuclear Information System (INIS)

    Krupiński, Mariusz; Janicki, Tomasz; Pałecz, Bartłomiej; Długoński, Jerzy

    2014-01-01

    Highlights: • A. versicolor is able to degrade 4-n-NP as the sole source of carbon and energy. • 4-n-NP removal by A. versicolor was accompanied by the formation of metabolites. • Radioactive experiments show complete 4-n-NP mineralization by A. versicolor. • 4-n-NP initiates heat production in the A. versicolor spores. - Abstract: 4-n-Nonylphenol (4-n-NP) is an environmental pollutant with endocrine-disrupting activities that is formed during the degradation of nonylphenol polyethoxylates, which are widely used as surfactants. Utilization of 4-n-NP by the filamentous fungus Aspergillus versicolor as the sole carbon and energy source was investigated. By means of gas chromatography–mass spectrometry, we showed that in the absence of any carbon source other than 4-n-NP in the medium, A. versicolor completely removed the xenobiotic (100 mg L −1 ) after 3 d of cultivation. Moreover, mass spectrometric analysis of intracellular extracts led to the identification of eight intermediates. The mineralization of the xenobiotic in cultures supplemented with 4-n-NP [ring- 14 C(U)] as a growth substrate was also assessed. After 3 d of incubation, approximately 50% of the initially applied radioactivity was recovered in the form of 14 CO 2 , proving that this xenobiotic was completely metabolized and utilized by A. versicolor as a carbon source. Based on microscopic analysis, A. versicolor is capable of germinating spores under such conditions. To confirm these observations, a microcalorimetric method was used. The results show that even the highest amount of 4-n-NP initiates heat production in the fungal samples, proving that metabolic processes were affected by the use of 4-n-NP as an energetic substrate

  6. Biodegradation and utilization of 4-n-nonylphenol by Aspergillus versicolor as a sole carbon and energy source

    Energy Technology Data Exchange (ETDEWEB)

    Krupiński, Mariusz; Janicki, Tomasz [Department of Industrial Microbiology and Biotechnology, University of Lodz, Banacha 12/16, 90-237 Łódź (Poland); Pałecz, Bartłomiej [Department of Physical Chemistry, University of Lodz, Pomorska 165, 90-236 Łódź (Poland); Długoński, Jerzy, E-mail: jdlugo@biol.uni.lodz.pl [Department of Industrial Microbiology and Biotechnology, University of Lodz, Banacha 12/16, 90-237 Łódź (Poland)

    2014-09-15

    Highlights: • A. versicolor is able to degrade 4-n-NP as the sole source of carbon and energy. • 4-n-NP removal by A. versicolor was accompanied by the formation of metabolites. • Radioactive experiments show complete 4-n-NP mineralization by A. versicolor. • 4-n-NP initiates heat production in the A. versicolor spores. - Abstract: 4-n-Nonylphenol (4-n-NP) is an environmental pollutant with endocrine-disrupting activities that is formed during the degradation of nonylphenol polyethoxylates, which are widely used as surfactants. Utilization of 4-n-NP by the filamentous fungus Aspergillus versicolor as the sole carbon and energy source was investigated. By means of gas chromatography–mass spectrometry, we showed that in the absence of any carbon source other than 4-n-NP in the medium, A. versicolor completely removed the xenobiotic (100 mg L{sup −1}) after 3 d of cultivation. Moreover, mass spectrometric analysis of intracellular extracts led to the identification of eight intermediates. The mineralization of the xenobiotic in cultures supplemented with 4-n-NP [ring-{sup 14}C(U)] as a growth substrate was also assessed. After 3 d of incubation, approximately 50% of the initially applied radioactivity was recovered in the form of {sup 14}CO{sub 2}, proving that this xenobiotic was completely metabolized and utilized by A. versicolor as a carbon source. Based on microscopic analysis, A. versicolor is capable of germinating spores under such conditions. To confirm these observations, a microcalorimetric method was used. The results show that even the highest amount of 4-n-NP initiates heat production in the fungal samples, proving that metabolic processes were affected by the use of 4-n-NP as an energetic substrate.

  7. Responses of Meteorus Versicolor Wesm. (Hymenoptera, Braconidae) to the Population Density of Tephrina Arenacearia Den. et Schiff. (Lepidoptera. Geomatridae)

    OpenAIRE

    GUDRAT, Kerimova Ilhama; AKHMED, Abdinbekova Arifa

    1999-01-01

    The purpose of this research was the determination of the effectiveness of Meteorus versicolor Wesm. in the control of Tephrina arenacearia Den. et Schiff. We studied the behavioral responses of this braconid to changes the number of hosts in laboratory conditions. It was revealed that this parasitoid is distributed proportionally to host population density. M. versicolor can parasitize both the single and accumulated larvae of the host.

  8. The response of Dendroctonus valens (Coleoptera: Scolytidae) and Temnochila chlorodia (Coleoptera: Trogossitidae) to Ips paraconfusus (Coleoptera: Scolytidae) pheromone components and verbenone

    Science.gov (United States)

    Christopher J. Fettig; Stepehen R. McKelvey; Christopher P. Dabney; Robert R. Borys

    2007-01-01

    The red turpentine beetle, Dendroctonus valens LeConte, 1860 (Coleoptera: Curculionidae, Scolytinae), is a common bark beetle species found throughout much of North America and China. In 2004, we observed that California fivespined ips, Ips paraconfusus Lanier, 1970 (Coleoptera: Curculionidae, Scolytinae), attack densities in logging debris were inversely related to D...

  9. Role of Laccase and Low Molecular Weight Metabolites from Trametes versicolor in Dye Decolorization

    Directory of Open Access Journals (Sweden)

    Diego Moldes

    2012-01-01

    Full Text Available The studies regarding decolorization of dyes by laccase may not only inform about the possible application of this enzyme for environmental purposes, but also may provide important information about its reaction mechanism and the influence of several factors that could be involved. In this paper, decolorization of crystal violet and phenol red was carried out with different fractions of extracellular liquids from Trametes versicolor cultures, in order to describe the role of laccase in this reaction. Moreover, the possible role of the low molecular weight metabolites (LMWMs also produced by the fungus was evaluated. The results confirm the existence of a nonenzymatic decolorization factor, since the nonprotein fraction of the extracellular liquids from cultures of T. versicolor has shown decolorization capability. Several experiments were performed in order to identify the main compounds related to this ability, which are probably low molecular weight peroxide compounds.

  10. Aroma Characterization and Safety Assessment of a Beverage Fermented by Trametes versicolor.

    Science.gov (United States)

    Zhang, Yanyan; Fraatz, Marco Alexander; Müller, Julia; Schmitz, Hans-Joachim; Birk, Florian; Schrenk, Dieter; Zorn, Holger

    2015-08-12

    A cereal-based beverage was developed by fermentation of wort with the basidiomycete Trametes versicolor. The beverage possessed a fruity, fresh, and slightly floral aroma. The volatiles of the beverage were isolated by liquid-liquid extraction (LLE) and additionally by headspace solid phase microextraction (HS-SPME). The aroma compounds were analyzed by a gas chromatography system equipped with a tandem mass spectrometer and an olfactory detection port (GC-MS/MS-O) followed by aroma (extract) dilution analysis. Thirty-four different odor impressions were perceived, and 27 corresponding compounds were identified. Fifteen key odorants with flavor dilution (FD) factors ranging from 8 to 128 were quantitated, and their respective odor activity values (OAVs) were calculated. Six key odorants were synthesized de novo by T. versicolor. Furthermore, quantitative changes during the fermentation process were analyzed. To prepare for the market introduction of the beverage, a comprehensive safety assessment was performed.

  11. Comparative study of oral and topical ketoconazole therapy in pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Nagpal V

    2003-07-01

    Full Text Available Introduction: Both topical and systemic ketoconazole are reported to be effective against pityriasis versicolor. Material and Methods: Forty patients suffering from pityriasis versicolor were treated either with oral ketoconazole 200 mg per day or 2% ketoconazole cream topically once daily for 2 weeks. Results: On global assessment, after 2 weeks of start of therapy, 18 (90% out of 20 patients treated with oral ketoconazole were cured while 2 patients had considerable residual disease. In the ketoconazole cream group, 16 (80% out of 20 patients were cured and 4 patients had considerable residual disease. Conclusion: No significant difference was observed in the response rates in the two groups. Relapse occurred in two patients of the systemic ketoconazole group and six patients of the topical ketoconazole group during the follow-up period of three months.

  12. Sociogenetic structure of Polistes (Aphanilopterus versicolor Olivier, 1791 colonies (Hymenoptera, Vespidae, Polistini

    Directory of Open Access Journals (Sweden)

    Keize Nagamati Junior

    2010-01-01

    Full Text Available The observation of two distinct, well-defined oviposition areas in nests of the primitively eusocial wasp Polistes versicolor suggests the presence of multiple egg-layers and territorial behaviors. Electrophoretic analysis of enzyme loci in pupae from 35 colonies revealed an average observed heterozygosity of 0.10 and the existence of private polymorphisms, thereby indicating a low dispersion in this species. No evidence of diploid males was found. Phenotypic segregation analysis revealed the presence of more than one egg-laying female in 15 out of 35 colonies, as well as spatially preferential oviposition in 2 out of 13 nests, with distinct oviposition areas. Genetic relatedness estimates for brood were lower than expected for haplodiploid species under monogynous conditions (r = 0.75 for female broods and r = 0.5 for male in 4 of those 13 nests, thereby inferring complex sociogenetic structuring in Polistes versicolor colonies.

  13. Cyclo-(trp-phe diketopiperazines from the endophytic fungus Aspergillus versicolor isolated from Piper aduncum

    Directory of Open Access Journals (Sweden)

    Juliana R. Gubiani

    Full Text Available Six known compounds, three peptide derivatives: cyclo-(tryptophyl-phenylalanyl (2, diketopiperazine dimer WIN 64821 (3 and 3-hydroxy-15H-tryptophenaline (4, one adenine derivative: 2-hydroxy-6-N-isopentenyl-adenine (5, one phtalide derivative: 4-methoxyphtalide (1 and one benzoic acid derivative: 3-hydroxy-4-(1-hydroxy-1,5-dimethyl-hexyl benzoic acid (6, were isolated from the ethyl acetate extract of the endophytic fungus Aspergillus versicolor associated with the Piper aduncum plant. Their structures were determined on the basis of detailed interpretation of 1 D and 2D NMR spectra and in comparison with works reported in the literature. This paper, in effect, deals with the first report of these compounds in A. versicolor.

  14. White piedra, black piedra, tinea versicolor, and tinea nigra: contribution to the diagnosis of superficial mycosis.

    Science.gov (United States)

    Veasey, John Verrinder; Avila, Ricardo Bertozzi de; Miguel, Barbara Arruda Fraletti; Muramatu, Laura Hitomi

    2017-01-01

    Superficial mycoses are fungal infections restricted to the stratum corneum and to the hair shafts, with no penetration in the epidermis; they are: white piedra, black piedra, tinea versicolor, and tinea nigra. This study presents images of mycological tests performed in the laboratory, as well as exams performed at the authors office, in order to improve the dermatologist's knowledge about the diagnosis of these dermatoses, which are common in many countries.

  15. White piedra, black piedra, tinea versicolor, and tinea nigra: contribution to the diagnosis of superficial mycosis*

    Science.gov (United States)

    Veasey, John Verrinder; de Avila, Ricardo Bertozzi; Miguel, Barbara Arruda Fraletti; Muramatu, Laura Hitomi

    2017-01-01

    Superficial mycoses are fungal infections restricted to the stratum corneum and to the hair shafts, with no penetration in the epidermis; they are: white piedra, black piedra, tinea versicolor, and tinea nigra. This study presents images of mycological tests performed in the laboratory, as well as exams performed at the authors office, in order to improve the dermatologist's knowledge about the diagnosis of these dermatoses, which are common in many countries. PMID:29186263

  16. The Evolution of Personally Disadvantageous Punishment among Cofoundresses of the Ant Acromyrmex Versicolor

    OpenAIRE

    Cabrales, Antonio; Pollock, Gregory B.; Rissing, Steven W.

    1998-01-01

    Cofoundresses of the desert fungus garden ant Acromyrmex versicolor exhibit a forager specialist who subsumes all foraging risk prior to first worker eclosion (Rissing et al. 1989). In an experiment designed to mimic a "cheater" who refuses foraging assignment when her lot, cofoundresses delayed/failed to replace their forager, often leading to demise of their garden (Rissing et al. 1996). The cheater on task assignment is harmed, but so too is the punisher, as all will die without a healthy ...

  17. Symbiosis initiation in the bacterially luminous sea urchin cardinalfish Siphamia versicolor.

    Science.gov (United States)

    Dunlap, P V; Gould, A L; Wittenrich, M L; Nakamura, M

    2012-09-01

    To determine how each new generation of the sea urchin cardinalfish Siphamia versicolor acquires the symbiotic luminous bacterium Photobacterium mandapamensis, and when in its development the S. versicolor initiates the symbiosis, procedures were established for rearing S. versicolor larvae in an aposymbiotic state. Under the conditions provided, larvae survived and developed for 28 days after their release from the mouths of males. Notochord flexion began at 8 days post release (dpr). By 28 dpr, squamation was evident and the caudal complex was complete. The light organ remained free of bacteria but increased in size and complexity during development of the larvae. Thus, aposymbiotic larvae of the fish can survive and develop for extended periods, major components of the luminescence system develop in the absence of the bacteria and the bacteria are not acquired directly from a parent, via the egg or during mouth brooding. Presentation of the symbiotic bacteria to aposymbiotic larvae at 8-10 dpr, but not earlier, led to initiation of the symbiosis. Upon colonization of the light organ, the bacterial population increased rapidly and cells forming the light-organ chambers exhibited a differentiated appearance. Therefore, the light organ apparently first becomes receptive to colonization after 1 week post-release development, the symbiosis is initiated by bacteria acquired from the environment and bacterial colonization induces morphological changes in the nascent light organ. The abilities to culture larvae of S. versicolor for extended periods and to initiate the symbiosis in aposymbiotic larvae are key steps in establishing the experimental tractability of this highly specific vertebrate and microbe mutualism. © 2012 The Authors. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  18. Tramesan, a novel polysaccharide from Trametes versicolor. Structural characterization and biological effects.

    Directory of Open Access Journals (Sweden)

    Marzia Scarpari

    Full Text Available Mushrooms represent a formidable source of bioactive compounds. Some of these may be considered as biological response modifiers; these include compounds with a specific biological function: antibiotics (e.g. plectasin, immune system stimulator (e,g, lentinan, antitumor agents (e.g. krestin, PSK and hypolipidemic agents (e.g. lovastatin inter alia. In this study, we focused on the Chinese medicinal mushroom "yun zhi", Trametes versicolor, traditionally used for (cit. "replenish essence and qi (vital energy". Previous studies indicated the potential activity of extracts from culture filtrate of asexual mycelia of T. versicolor in controlling the growth and secondary metabolism (e.g. mycotoxins of plant pathogenic fungi. The quest of active principles produced by T. versicolor, allowed us characterising an exo-polysaccharide released in its culture filtrate and naming it Tramesan. Herein we evaluate the biological activity of Tramesan in different organisms: plants, mammals and plant pathogenic fungi. We suggest that the bioactivity of Tramesan relies mostly on its ability to act as pro antioxidant molecule regardless the biological system on which it was applied.

  19. Prevalence of different Malassezia species in pityriasis versicolor in central India

    Directory of Open Access Journals (Sweden)

    Chaudhary Rahul

    2010-01-01

    Full Text Available Background: In the last 10 years, different studies have shown interesting geographical variations in the prevalence of different Malassezia species in pityriasis versicolor. Aim: Identification of Malassezia species isolated from patients with pityriasis versicolor. Methods: In 100 patients with pityriasis versicolor, Malassezia species were identified by culture in Sabouraud′s dextrose agar containing cycloheximide with olive oil overlay and modified Dixon agar and by doing biochemical tests (catalase reaction, assimilation of glycine, and Tween utilisation tests. Results: In 10 patients, 10% KOH smear was negative, while in 90 patients the smear showed characteristic "spaghetti and meatball" appearance. Of these 90 cases, growth was obtained on modified Dixon′s agar in 87 cases. Fifty of the isolates (57.5% were M. globosa, 15 (17.2% were M. sympodialis, seven (8.0% were suspected M. sympodialis, 6 (6.9% each of the isolates were M. furfur and M. obtusa, and three (3.4% isolates were M. restricta. Conclusion: M. globosa was the most common species, followed by M. sympodialis, M. furfur, M. obtusa, and M. restricta.

  20. Prevalence of different Malassezia species in pityriasis versicolor in central India.

    Science.gov (United States)

    Chaudhary, Rahul; Singh, Sanjay; Banerjee, Tuhina; Tilak, Ragini

    2010-01-01

    In the last 10 years, different studies have shown interesting geographical variations in the prevalence of different Malassezia species in pityriasis versicolor. Identification of Malassezia species isolated from patients with pityriasis versicolor. In 100 patients with pityriasis versicolor, Malassezia species were identified by culture in Sabouraud's dextrose agar containing cycloheximide with olive oil overlay and modified Dixon agar and by doing biochemical tests (catalase reaction, assimilation of glycine, and Tween utilisation tests). In 10 patients, 10% KOH smear was negative, while in 90 patients the smear showed characteristic "spaghetti and meatball" appearance. Of these 90 cases, growth was obtained on modified Dixon's agar in 87 cases. Fifty of the isolates (57.5%) were M. globosa, 15 (17.2%) were M. sympodialis, seven (8.0%) were suspected M. sympodialis, 6 (6.9%) each of the isolates were M. furfur and M. obtusa, and three (3.4%) isolates were M. restricta. M. globosa was the most common species, followed by M. sympodialis, M. furfur, M. obtusa, and M. restricta.

  1. Genetic diversity of Begonia versicolor (Begoniaceae, a narrow endemic species in southeast Yunnan of China

    Directory of Open Access Journals (Sweden)

    Chun Li

    2018-03-01

    Full Text Available Begonia versicolor Irmscher, a narrow endemic Begonia species in southeast Yunnan of China, is a wonderful ornamental plant with huge diversity in colored foliage. To investigate its variations, the genetic diversity and population structure were studied based on 56 individuals sampled from four localities using 12 polymorphic microsatellite loci transferred from other species of Begonia. The results showed a relatively low level of genetic diversity in B. versicolor comparing with other species of Begonia using microsatellite. Positive inbreeding coefficient (FIS values were found in three populations (SWC, XPZ and DSD. AMOVA analysis indicated that genetic variations occurred mainly within populations (55.9% rather than among populations (9.7% and among groups (34.4%. Four populations were grouped into two clusters based on STRUCTURE. AMOVA and STRUCTURE analysis showed a high level and significant genetic differentiation in the populations of B. versicolor. Based on its genetic status and rarity in the wild, the sustainable in-situ and ex-situ conservation strategies should be urgently carried out to protect this species with high horticultural and scientific values.

  2. Transcriptional Responses in the Hemiparasitic Plant Triphysaria versicolor to Host Plant Signals1[w

    Science.gov (United States)

    Matvienko, Marta; Torres, Manuel J.; Yoder, John I.

    2001-01-01

    Parasitic plants in the Scrophulariaceae use chemicals released by host plant roots to signal developmental processes critical for heterotrophy. Haustoria, parasitic plant structures that attach to and invade host roots, develop on roots of the hemiparasitic plant Triphysaria versicolor within a few hours of exposure to either maize (Zea mays) root exudate or purified haustoria-inducing factors. We prepared a normalized, subtractive cDNA library enriched for transcripts differentially abundant in T. versicolor root tips treated with the allelopathic quinone 2,6-dimethoxybenzoquinone (DMBQ). Northern analyses estimated that about 10% of the cDNAs represent transcripts strongly up-regulated in roots exposed to DMBQ. Northern and reverse northern analyses demonstrated that most DMBQ-responsive messages were similarly up-regulated in T. versicolor roots exposed to maize root exudates. From the cDNA sequences we assembled a unigene set of 137 distinct transcripts and assigned functions by homology comparisons. Many of the proteins encoded by the transcripts are predicted to function in quinone detoxification, whereas others are more likely associated with haustorium development. The identification of genes transcriptionally regulated by haustorium-inducing factors provides a framework for dissecting genetic pathways recruited by parasitic plants during the transition to heterotrophic growth. PMID:11553755

  3. Flavonoids Promote Haustoria Formation in the Root Parasite Triphysaria versicolor1

    Science.gov (United States)

    Albrecht, Huguette; Yoder, John I.; Phillips, Donald A.

    1999-01-01

    Parasitic plants in the Scrophulariaceae develop infective root structures called haustoria in response to chemical signals released from host-plant roots. This study used a simple in vitro assay to characterize natural and synthetic molecules that induce haustoria in the facultative parasite Triphysaria versicolor. Several phenolic acids, flavonoids, and the quinone 2,6-dimethoxy-p-benzoquinone induced haustoria in T. versicolor root tips within hours after treatment. The concentration at which different molecules were active varied widely, the most active being 2,6-dimethoxy-p-benzoquinone and the anthocyanidin peonidin. Maize (Zea mays) seeds are rich sources of molecules that induce T. versicolor haustoria in vitro, and chromatographic analyses indicated that the active molecules present in maize-seed rinses include anthocyanins, other flavonoids, and simple phenolics. The presence of different classes of inducing molecules in seed rinses was substantiated by the observation that maize kernels deficient in chalcone synthase, a key enzyme in flavonoid biosynthesis, released haustoria-inducing molecules, although at reduced levels compared with wild-type kernels. We discuss these results in light of existing models for host perception in the related parasitic plant Striga. PMID:9952454

  4. Tanyproctini (Coleoptera: Scarabaeidae: Melolonthinae) of Socotra Island

    Czech Academy of Sciences Publication Activity Database

    Král, D.; Sehnal, R.; Bezděk, Aleš

    2012-01-01

    Roč. 52, suppl. 2 (2012), s. 153-182 ISSN 0374-1036 Grant - others:Ministry of Education, Youth and Sports (CZ) LA10036/MSMT Institutional support: RVO:60077344 Keywords : Coleoptera * Scarabaeidae * Melolonthinae Subject RIV: EG - Zoology Impact factor: 0.963, year: 2012 http://www.aemnp.eu/PDF/52_s2/52_S2_153.pdf

  5. Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tehran, Iran

    Directory of Open Access Journals (Sweden)

    Zeraati Hojjat

    2004-05-01

    Full Text Available Abstract Background Pityriasis versicolor is a superficial infection of the stratum corneum which caused by a group of yeasts formerly named pityrosporium. The taxonomy of these lipophilic yeasts has recently been modified and includes seven species referred as Malassezia. The aim of this study is to compare the distribution of Malassezia species isolated from pityriasis versicolor lesions and those isolated from healthy skins. Methods Differentiation of all malassezia species performed using morphological features and physiological test including catalase reaction, Tween assimilation test and splitting of esculin. Results In pityriasis versicolor lesions, the most frequently isolated species was M. globosa (53.3%, followed by M. furfur (25.3%, M. sympodialis(9.3%, M. obtusa (8.1% and M. slooffiae (4.0%. The most frequently isolated species in the skin of healthy individuals were M. globosa, M. sympodialis, M. furfur, M. sloofiae and M. restricta which respectively made up 41.7%, 25.0%, 23.3%, 6.7% and 3.3% of the isolated species. Conclusions According to our data, M. globosa was the most prevalent species in the skin of healthy individuals which recovered only in the yeast form. However, the Mycelial form of M. globosa was isolated as the dominant species from pityriasis versicolor lesions. Therefore, the role of predisposing factors in the conversion of this yeast to mycelium and its subsequent involvement in pityriasis versicolor pathogenicity should be considered.

  6. Heterologous expression of trametes versicolor laccase in pichia pastoris and aspergillus niger

    CSIR Research Space (South Africa)

    Bohlin, C

    2006-09-01

    Full Text Available primarily for screening purposes. With A. niger, high levels of laccase (2700 U/L) were produced using a min- imal medium containing sucrose and yeast extract. Recombinant laccase from A. niger harboring the lcc2 cDNA was purified to homogeneity...). Methods Microbial Strains and Recombinant DNA The lcc1 and lcc2 cDNA genes from T. (Coriolus, Polyporus) versicolor (9–11) were used in the construction of plasmids for expression of laccases in P. pastoris and A. niger. For the expression in P...

  7. Gravimetric screening method for fungal decay of paper: inoculation with Trametes versicolor.

    Science.gov (United States)

    Råberg, Ulrika; Hafrén, Jonas

    2009-10-01

    The European standard test EN 113 for fungal degradation of solid wood has been adapted for degradation of paper by white rot fungus (Trametes versicolor). Fungal degradation of paper sheets may potentially be used for screening different wood preservatives on paper instead of solid wood. The paper samples showed higher relative mass losses compared to wood, and samples pretreated with boric acid, copper sulfate and polymerized linseed oil were successfully tested for biodegradation using the paper sheet method. The results on paper degradation were compared with wood, both as wood blocks (according to standard test) and wood cut in sections forming layered structures mimicking paper layers.

  8. Heavy metals in carabids (Coleoptera, Carabidae

    Directory of Open Access Journals (Sweden)

    Ruslan Butovsky

    2011-05-01

    Full Text Available Carabid beetles (Coleoptera, Carabidae are one of the most studied soil groups in relation to heavy metal (HM accumulation and use for bioindication of environmental pollution. Accumulation of Zn and Cu in carabid beetles was species-, sex- and trophic group-specific. No differences were found in HM contents between omnivorous and carnivorous species. The use of carabid beetles as indicators of HM accumulation appears to be rather limited.

  9. Study On Selenium Accumulation In Turkey Tail Fungus (Trametes Versicolor) By Using Instrumental Neutron Activation Analysis

    International Nuclear Information System (INIS)

    Le Xuam Tham; Ho Van Hai; Bui Thi Minh Hai; Nguyen Giang; Nguyen Thi Dieu Hanh

    2008-01-01

    Medicinal mushroom Kawaratake Trametes versicolor strain obtained from Chiba University, Japan, was fermented at stationary Erlenmayer flasks 250 ml, containing PG media with Se (10 ppm as selenate) and without Se (control) supplement, incubated at 32-34 o C (room temperature in Ho Chi Minh City). Selenium was added as dissolve selenate Na 2 SeO 4 , in which Se was enriched with 74 Se. Under neutron fluxes in nuclear reactor 74 Se will be activated into 75 Se emitted gamma rays (n-γ reaction), recorded for calculations of Se contents in the samples. Cultivation of Kawaratake on mixed substrates based sawdusts supplemented with Se by injecting directly into center region of the substrate based on rubber tree sawdust. Analysis of Se contents in fungal biomass harvested by using INAA with neutron flux at 10 12 cm -2 .s -1 in Nuclear Reactor at Dalat City, Vietnam. The results obtained with Trametes versicolor showed Se levels in mycelial biomass up to 600-1500 ppm, while in the control (without Se supplement), only trace of Se (1 ppm more or less) was found. It would be related to the researches on configurations of complex of polysaccharides and proteins in biomass with high bioactivities. Se would substitute S for some due structures and enhance their antioxydant activities. (author)

  10. Corolla morphology influences diversification rates in bifid toadflaxes (Linaria sect. Versicolores)

    Science.gov (United States)

    Fernández-Mazuecos, Mario; Blanco-Pastor, José Luis; Gómez, José M.; Vargas, Pablo

    2013-01-01

    Background and Aims The role of flower specialization in plant speciation and evolution remains controversial. In this study the evolution of flower traits restricting access to pollinators was analysed in the bifid toadflaxes (Linaria sect. Versicolores), a monophyletic group of ∼30 species and subspecies with highly specialized corollas. Methods A time-calibrated phylogeny based on both nuclear and plastid DNA sequences was obtained using a coalescent-based method, and flower morphology was characterized by means of morphometric analyses. Directional trends in flower shape evolution and trait-dependent diversification rates were jointly analysed using recently developed methods, and morphological shifts were reconstructed along the phylogeny. Pollinator surveys were conducted for a representative sample of species. Key Results A restrictive character state (narrow corolla tube) was reconstructed in the most recent common ancestor of Linaria sect. Versicolores. After its early loss in the most species-rich clade, this character state has been convergently reacquired in multiple lineages of this clade in recent times, yet it seems to have exerted a negative influence on diversification rates. Comparative analyses and pollinator surveys suggest that the narrow- and broad-tubed flowers are evolutionary optima representing divergent strategies of pollen placement on nectar-feeding insects. Conclusions The results confirm that different forms of floral specialization can lead to dissimilar evolutionary success in terms of diversification. It is additionally suggested that opposing individual-level and species-level selection pressures may have driven the evolution of pollinator-restrictive traits in bifid toadflaxes. PMID:24142920

  11. Advanced oxidation of benzene, toluene, ethylbenzene and xylene isomers (BTEX) by Trametes versicolor.

    Science.gov (United States)

    Aranda, Elisabet; Marco-Urrea, Ernest; Caminal, Gloria; Arias, María E; García-Romera, Inmaculada; Guillén, Francisco

    2010-09-15

    Advanced oxidation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylene (BTEX) by the extracellular hydroxyl radicals (*OH) generated by the white-rot fungus Trametes versicolor is for the first time demonstrated. The production of *OH was induced by incubating the fungus with 2,6-dimethoxy-1,4-benzoquinone (DBQ) and Fe3+-EDTA. Under these conditions, *OH were generated through DBQ redox cycling catalyzed by quinone reductase and laccase. The capability of T. versicolor growing in malt extract medium to produce *OH by this mechanism was shown during primary and secondary metabolism, and was quantitatively modulated by the replacement of EDTA by oxalate and Mn2+ addition to DBQ incubations. Oxidation of BTEX was observed only under *OH induction conditions. *OH involvement was inferred from the high correlation observed between the rates at which they were produced under different DBQ redox cycling conditions and those of benzene removal, and the production of phenol as a typical hydroxylation product of *OH attack on benzene. All the BTEX compounds (500 microM) were oxidized at a similar rate, reaching an average of 71% degradation in 6 h samples. After this time oxidation stopped due to O2 depletion in the closed vials used in the incubations. Copyright 2010 Elsevier B.V. All rights reserved.

  12. Effect Of Metal Ions On Triphenylmethane Dye Decolorization By Laccase From Trametes Versicolor

    Directory of Open Access Journals (Sweden)

    Chmelová Daniela

    2015-12-01

    Full Text Available The aim of this study was investigate the influence of different metal ions on laccase activity and triphenylmethane dye decolorization by laccase from white-rot fungus Trametes versicolor. Laccase activity was inhibited by monovalent ions (Li+, Na+, K+ and Ag+ but the presence of divalent ions increased laccase activity at the concentration of 10 mmol/l. The effect of metal ions on decolorization of triphenylmethane dyes with different structures namely Bromochlorophenol Blue, Bromophenol Blue, Bromocresol Blue and Phenol Red was tested. The presence of metal ions (Na+, K+, Mg2+, Ca2+, Ba2+, Mn2+, Zn2+ slightly decreased triphenylmethane dye decolorization by laccase from T. versicolor except Na+ and Mg2+, which caused the increase of decolorization for all tested dyes. Decolorization of selected dyes showed that the presence of low-molecular-weight compounds is necessary for effective decolorization. Hydroxybenzotriazole (HBT is the most frequently used. Although HBT belongs to most frequently used redox mediator and generally increase decolorization efficiency, so its presence decreased decolorization percentage of Bromophenol Blue and Bromochlorophenol Blue, the influence of metal ions to dye decolorization by laccase has the similar course with or without presence of redox mediator HBT.

  13. Real-time PCR analysis of carbon catabolite repression of cellobiose gene transcription in Trametes versicolor

    Energy Technology Data Exchange (ETDEWEB)

    Stapleton, P. C.; O' Mahoney, J.; Dobson, A. D. W. [National University of Ireland, Microbiology Department, Cork (Ireland)

    2004-02-01

    Previous reports indicate that in white rot fungi such as Trametes versicolor, the production of cellobiose dehydrogenase (CDH), an extracellular haemo-flavo-enzyme, is subject to carbon catabolite repression by both glucose and maltose, and that the repression is mediated at the transcriptional level. This paper describes the results of an investigation of CDH gene transcription in cellulolytic cultures of T. versicolor, in the presence of other additional carbon sources such as glucose, arabinose, and xylose. Using real time polymerase chain reaction (RT-PCR) assay methods in the presence of these other additional carbon sources, the levels of repression observed are quantitatively determined in an effort to obtain more accurate measurements of carbon catabolite repression of CDH production in this ligninolytic fungus. Ninety-six hours after addition, results of the analysis showed reduction in CDH transcript levels of 19-fold for galactose, 92-fold for arabinose and 114-fold for xylose. The greatest repressive effect was exhibited by glucose. In this case the reduction in CDH transcript levels was 3400-fold. CDH plays an important role in lignin degradation, and there is also substantial interest in the biotechnological applications of CDH, most particularly in the pulp and paper industry. 24 refs., 4 figs.

  14. Proteomics investigation reveals cell death-associated proteins of basidiomycete fungus Trametes versicolor treated with Ferruginol.

    Science.gov (United States)

    Chen, Yu-Han; Yeh, Ting-Feng; Chu, Fang-Hua; Hsu, Fu-Lan; Chang, Shang-Tzen

    2015-01-14

    Ferruginol has antifungal activity against wood-rot fungi (basidiomycetes). However, specific research on the antifungal mechanisms of ferruginol is scarce. Two-dimensional gel electrophoresis and fluorescent image analysis were employed to evaluate the differential protein expression of wood-rot fungus Trametes versicolor treated with or without ferruginol. Results from protein identification of tryptic peptides via liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS) analyses revealed 17 protein assignments with differential expression. Downregulation of cytoskeleton β-tubulin 3 indicates that ferruginol has potential to be used as a microtubule-disrupting agent. Downregulation of major facilitator superfamily (MFS)–multiple drug resistance (MDR) transporter and peroxiredoxin TSA1 were observed, suggesting reduction in self-defensive capabilities of T. versicolor. In addition, the proteins involved in polypeptide sorting and DNA repair were also downregulated, while heat shock proteins and autophagy-related protein 7 were upregulated. These observations reveal that such cellular dysfunction and damage caused by ferruginol lead to growth inhibition and autophagic cell death of fungi.

  15. Parasitism and olfactory responses of Dastarcus helophoroides (Coleoptera: Bothrideridae) to different Cerambycid hosts

    Science.gov (United States)

    Jian-Rong Wei; Zhong-Qi Yang; Therese M. Poland; Jia-Wei. Du

    2009-01-01

    Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an important natural enemy of longhorned beetles (Coleoptera: Cerambycidae). It is distributed throughout most Provinces in China. We investigated whether there were differences among D. helophoroides populations collected from different hosts in different...

  16. Biomarker analysis of American toad (Anaxyrus americanus) and grey tree frog (Hyla versicolor) tadpoles following exposure to atrazine.

    Science.gov (United States)

    The objective of the current study was to use a biomarker-based approach to investigate the influence of atrazine exposure on American toad (Anaxyrus americanus) and grey tree frog (Hyla versicolor) tadpoles. Atrazine is one of the most frequently detected herbicides in environme...

  17. Leaf removal by sesarmid crabs in Bangrong mangrove forest, Phuket, Thailand; with emphasis on the feeding ecology of Neoepisesarma versicolor

    Science.gov (United States)

    Thongtham, Nalinee; Kristensen, Erik; Puangprasan, Som-Ying

    2008-12-01

    Field measurements on leaf removal by populations of sesarmid crabs at different locations in the Bangrong mangrove forest, Phuket, Thailand, indicated that crabs on average can remove 87% of the daily leaf litter fall by ingestion or burial. The removal rate is correlated positively with the number of crab burrows and negatively with tidal inundation time. The results from the field were supplemented with observations on the behavior of Neoepisesarma versicolor in laboratory microcosms and a mangrove mesocosm. N. versicolor feeds primarily at night and total time spent feeding was up to an order of magnitude higher in the artificial microcosms than under simulated in situ conditions in the mesocosm. Most of the time during both day and night was spent resting near the entrance or inside burrows. N. versicolor mainly feeds on mangrove leaves and scraps of food material from the sediment surface. This is supported by examinations of stomach content, which showed that 62% is composed of higher plant material and 38% of detritus and mineral particles from the sediment. The nutritive value of leaves and detritus is insufficient to maintain crab growth. Sesarmid crabs may instead obtain the needed nutrients by occasional consumption of nitrogen-rich animal tissues, such as carcasses of fish and crustaceans, as indicated by the presence of animal remains in the stomach and the willingness of crabs to consume fish meat. Laboratory experiments on leaf consumption and leaf preferences of N. versicolor indicate that they preferentially feed on brown leaves, if available, followed by green and yellow leaves. If all species of sesarmid crabs in the Bangrong mangrove forest consume leaves at the same rate as N. versicolor, they could potentially ingest 52% of the total litter fall.

  18. New synonymy in Cuban Tilloclytus (Coleoptera: Cerambycidae: Anaglyptini)

    Science.gov (United States)

    Examination of holotypes of Tilloclytus (Coleoptera: Cerambycidae: Anaglyptini) in the Fernando de Zayas collection (Havana, Cuba) and the Museum of Comparative Zoology, Harvard University reveals that T. elongatus Zayas (1975) is a new synonym of T. rufipes Fisher (1942)....

  19. Coleoptera species of forensic importance from Brazil: an updated list

    Directory of Open Access Journals (Sweden)

    Lúcia Massutti de Almeida

    2015-12-01

    Full Text Available ABSTRACT A list of the Coleoptera of importance from Brazil, based on published records was compiled. The checklist contains 345 species of 16 families allocated to 16 states of the country. In addition, three species of two families are registered for the first time. The fauna of Coleoptera of forensic importance is still not entirely known and future collection efforts and taxonomic reviews could increase the number of known species considerably in the near future.

  20. Immatures of Acanthocinini (Coleoptera, Cerambycidae, Lamiinae

    Directory of Open Access Journals (Sweden)

    Sônia A. Casari

    2014-06-01

    Full Text Available Immatures of Acanthocinini (Coleoptera, Cerambycidae, Lamiinae. Larva and pupa of Eutrypanus dorsalis (Germar, 1928, collected in trunks of Pinus elliottii Engelm., and Paratenthras martinsi Monné, 1998, collected in spathes of Scheelea phalerata (Mart. ex Spreng. Burret, are described and illustrated. Larva and pupa of Lophopoeum timbouvae Lameere, 1884, collected in Hymenaea corbaril L., Enterolobium contortisiliquum (Vell. Morong and Pterogyne nitens Tul., are redescribed and illustrated. A table with all described immatures of Lamiinae, and a comparison among the immatures of Acanthocinini are presented. Biological notes and new records are also included.

  1. Aspergoterpenins A–D: Four New Antimicrobial Bisabolane Sesquiterpenoid Derivatives from an Endophytic Fungus Aspergillus versicolor

    Directory of Open Access Journals (Sweden)

    Zhi-Yong Guo

    2018-05-01

    Full Text Available Aspergoterpenins A–D (1–4, four new bisabolane sesquiterpenoid derivatives, were obtained from the endophytic fungus, Aspergillus versicolor, together with eight known compounds (5–12, and their structures were elucidated by a comprehensive analysis of their NMR (Nuclear Magnetic Resonance, MS (Mass Spectrum and CD (Circular Dichroism spectra. Aspergoterpenin A (1 was the first example with a characteristic ketal bridged-ring part in the degraded natural bisabolane-type sesquiterpene structures. The compounds 1–12 displayed no significant activities against four cancer cell lines (A549, Caski, HepG2 and MCF-7. Further, the antimicrobial activities to Erwinia carotovora sub sp. Carotovora were evaluated, and the results showed that compounds 1–12 displayed antimicrobial activities with MIC values ranging from 15.2 to 85.2 μg/mL.

  2. Comparative study of ketoconazole versus selenium sulphide shampoo in pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Aggarwal K

    2003-03-01

    Full Text Available Forty patients suffering from pityriasis versicolor were treated with either 2% ketoconazole shampoo (20 patients or 2.5% selenium sulphide shampoo (20 patients, once a week for three weeks. On global assessment after one month of start of therapy, 19 (95% out of 20 patients treated with ketoconazole shampoo were cured while one case had mild residual disease. In selenium sulphide shampoo group, 17 (85% out of 20 patients were cured, one had mild residual disease and two had considerable residual disease. No significant difference was observed in the response rates in the two groups. Relapse occurred in one patient of ketoconazole group and two patients of selenium sulphide group during the follow - up period of three months.

  3. Degradation of selected agrochemicals by the white rot fungus Trametes versicolor.

    Science.gov (United States)

    Mir-Tutusaus, Josep Anton; Masís-Mora, Mario; Corcellas, Cayo; Eljarrat, Ethel; Barceló, Damià; Sarrà, Montserrat; Caminal, Glòria; Vicent, Teresa; Rodríguez-Rodríguez, Carlos E

    2014-12-01

    Use of agrochemicals is a worldwide practice that exerts an important effect on the environment; therefore the search of approaches for the elimination of such pollutants should be encouraged. The degradation of the insecticides imiprothrin (IP) and cypermethrin (CP), the insecticide/nematicide carbofuran (CBF) and the antibiotic of agricultural use oxytetracycline (OTC) were assayed with the white rot fungus Trametes versicolor. Experiments with fungal pellets demonstrated extensive degradation of the four tested agrochemicals, at rates that followed the pattern IP>OTC>CP>CBF. In vitro assays with laccase-mediator systems showed that this extracellular enzyme participates in the transformation of IP but not in the cases of CBF and OTC. On the other hand, in vivo studies with inhibitors of cytochrome P450 revealed that this intracellular system plays an important role in the degradation of IP, OTC and CBF, but not for CP. The compounds 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (DCCA) and 3-phenoxybenzoic acid (PBA) were detected as transformation products of CP, as a result of the breakdown of the molecule. Meanwhile, 3-hydroxycarbofuran was detected as a transformation product of CBF; this metabolite tended to accumulate during the process, nonetheless, the toxicity of the system was effectively reduced. Simultaneous degradation of CBF and OTC showed a reduction in toxicity; similarly, when successive additions of OTC were done during the slower degradation of CBF, the fungal pellets were able to degrade both compounds. The simultaneous degradation of the four compounds successfully took place with minimal inhibition of fungal activity and resulted in the reduction of the global toxicity, thus supporting the potential use of T. versicolor for the treatment of diverse agrochemicals. Copyright © 2014 Elsevier B.V. All rights reserved.

  4. The evolution of asymmetric genitalia in Coleoptera.

    Science.gov (United States)

    Schilthuizen, Menno; de Jong, Paulien; van Beek, Rick; Hoogenboom, Tamara; Schlochtern, Melanie Meijer Zu

    2016-12-19

    The evolution of asymmetry in male genitalia is a pervasive and recurrent phenomenon across almost the entire animal kingdom. Although in some taxa the asymmetry may be a response to the evolution of one-sided, male-above copulation from a more ancestral female-above condition, in other taxa, such as Mammalia and Coleoptera, this explanation appears insufficient. We carried out an informal assessment of genital asymmetry across the Coleoptera and found that male genital asymmetry is present in 43% of all beetle families, and at all within-family taxonomic levels. In the most diverse group, Cucujiformia, however, genital asymmetry is comparatively rare. We also reconstructed the phylogeny of the leiodid tribe Cholevini, and mapped aspects of genital asymmetry on the tree, revealing that endophallus sclerites, endophallus, median lobe and parameres are, in a nested fashion, increasingly unlikely to have evolved asymmetry. We interpret these results in the light of cryptic female choice versus sexually antagonistic coevolution and advocate further ways in which the phenomenon may be better understood.This article is part of the themed issue 'Provocative questions in left-right asymmetry'. © 2016 The Authors.

  5. Tinea Versicolor

    Science.gov (United States)

    ... Registration General information Housing & travel Education Exhibit hall Mobile app 2019 Annual Meeting Derm Exam Prep Course ... SkinPAC State societies Scope of practice Truth in advertising NP/PA laws Action center Public and patients ...

  6. Ensaio terapêutico com o Ketoconazol na pitiríase versicolorTherapeutic trial with Ketoconazole in PV

    Directory of Open Access Journals (Sweden)

    Leila Dagher

    1982-11-01

    Full Text Available Experimentação terapêutico-laboratorial com o Ketoconazol na Pitiríase Versicolor, obtendo os seguintes resultados: - Curados, 16 (88,88% pacientes; Melhorados, 2 pacientes (11,11%, Inalterados e Piorados, nenhum caso (0,00%, no total de 18 doentes estudados. Do ponto de vista laboratorial, houve negativação dos exames micológicos em 100,00% dos casos.Eighteen patients with pityriasis versicolor were treated with a single daily dose of 200mg of Ketoconazole administered with a meal, for a period of 30 days. Evaluation was based on clinical and mycological responses. Clinical and parasitoligical cure was obtained in 16 (88,88% patients and marked clinical improvement in 2(11,11%. No undesirable side effects were observed in the treatment.

  7. Continuous degradation of a mixture of sulfonamides by Trametes versicolor and identification of metabolites from sulfapyridine and sulfathiazole

    International Nuclear Information System (INIS)

    Rodríguez-Rodríguez, Carlos E.; Jesús García-Galán, Ma.; Blánquez, Paqui; Díaz-Cruz, M. Silvia; Barceló, Damià; Caminal, Glòria; Vicent, Teresa

    2012-01-01

    Highlights: ► Degradation of sulfapyridine and sulfathiazole by Trametes versicolor was evaluated. ► The role of laccase and cytochrome P450 was determined. ► Degradation metabolites were identified for sulfapyridine (8) and sulfathiazole (5). ► A mixture of three sulfonamides was degraded in a continuous fluidized bed reactor. - Abstract: In this study, we assessed the degradation of the sulfonamides sulfapyridine (SPY) and sulfathiazole (STZ) by the white-rot fungus Trametes versicolor. Complete degradation was accomplished in fungal cultures at initial pollutant concentrations of approximately 10 mg L −1 , although a longer period of time was needed to completely remove STZ in comparison to SPY. When cytochrome P450 inhibitors were added to the fungal cultures, STZ degradation was partially suppressed, while no additional effect was observed for SPY. Experiments with purified laccase and laccase mediators caused the removal of greater than 75% of each antibiotic. Ultra-performance liquid chromatography-quadupole time of flight mass spectrometry (UPLC-QqTOF-MS) analyses allowed the identification of a total of eight degradation intermediates of SPY in both the in vivo and the laccase experiments, being its desulfonated moiety the commonly detected product. For STZ, a total of five products were identified. A fluidized bed reactor with T. versicolor pellets degraded a mixture of sulfonamides (SPY, STZ and sulfamethazine, SMZ) by greater than 94% each at a hydraulic residence time of 72 h. Because wastewater contains many diverse pollutants, these results highlight the potential of T. versicolor as a bioremediation agent not only for the removal of antibiotics but also for the elimination of a wide range of contaminants.

  8. Detection of Malassezia Species Isolated From Patients With Pityriasis Versicolor and Seborrheic Dermatitis Using Nested-PCR

    Directory of Open Access Journals (Sweden)

    Zarei Mahmoudabadi

    2014-12-01

    Full Text Available Background The species of the genus Malassezia are lipophilic and dimorphic yeasts that are regarded as part of the normal flora of the skin of humans and warm-blooded animals. These organisms are the cause of superficial mycosis in humans and other animals, and are common in pityriasis versicolor and seborrheic dermatitis. Objectives The purpose of this study was to determine the frequency of common Malassezia species in patients affected by pityriasis versicolor and seborrheic dermatitis using of the nested PCR method, in the city of Ahvaz. Patients and Methods In the present study, 85 samples from patients with pityriasis versicolor and seborrheic dermatitis were analyzed by the nested-PCR method. During the first stage, the internal transcribed spacer (ITS region from the ribosomal DNA was reproduced using primers ITS4-R and ITS1F-N. During the second stage, the product of the first step was used as DNA and using three special primer pairs, including Mf-F, 5.8SR and M.gl-F, 5.8SR and M.rt-F and M.rt-R, the inner part of the first phase was detected. Results The most common isolate was Malassezia furfur (51.3% followed by M. globosa (35.2% and M. restricta (13.5%. Amongst the 30 patients with seborrheic dermatitis, in 15 cases (65.2% M. restricta, in six cases (26.1% M. globosa and in two cases (8.7% M. furfur was detected and in seven patients no isolate was detected. Conclusions The nested-PCR is a rapid and repeatable method for identification of important Malassezia species and this method is recommended for use on more patients. In addition the most common agents of pityriasis versicolor and seborrheic dermatitis were M. furfur and M. restricta, respectively.

  9. Mating tests among geographically separated collections of the Trametes versicolor (Fr.) Pilát (Basidiomycetes, Polyporales) group

    Czech Academy of Sciences Publication Activity Database

    Tomšovský, Michal; Homolka, Ladislav

    2004-01-01

    Roč. 79, 3-4 (2004), s. 425-431 ISSN 0029-5035 R&D Projects: GA ČR GA526/02/1216; GA ČR GD206/03/H137 Institutional research plan: CEZ:AV0Z5020903 Keywords : trametes versicolor * mating tests Subject RIV: EE - Microbiology, Virology Impact factor: 0.594, year: 2004

  10. Continuous degradation of a mixture of sulfonamides by Trametes versicolor and identification of metabolites from sulfapyridine and sulfathiazole

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez-Rodriguez, Carlos E., E-mail: CarlosEsteban.Rodriguez@uab.cat [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 2060 San Jose (Costa Rica); Jesus Garcia-Galan, Ma. [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034, Barcelona (Spain); Blanquez, Paqui [Departament d' Enginyeria Quimica, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Diaz-Cruz, M. Silvia [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034, Barcelona (Spain); Barcelo, Damia [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034, Barcelona (Spain); Catalan Institute for Water Research (ICRA), Parc Cientific i Tecnologic de la Universitat de Girona, C/Emili Grahit, 101 Edifici H2O, E-17003 Girona (Spain); Caminal, Gloria [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Vicent, Teresa [Departament d' Enginyeria Quimica, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain)

    2012-04-30

    Highlights: Black-Right-Pointing-Pointer Degradation of sulfapyridine and sulfathiazole by Trametes versicolor was evaluated. Black-Right-Pointing-Pointer The role of laccase and cytochrome P450 was determined. Black-Right-Pointing-Pointer Degradation metabolites were identified for sulfapyridine (8) and sulfathiazole (5). Black-Right-Pointing-Pointer A mixture of three sulfonamides was degraded in a continuous fluidized bed reactor. - Abstract: In this study, we assessed the degradation of the sulfonamides sulfapyridine (SPY) and sulfathiazole (STZ) by the white-rot fungus Trametes versicolor. Complete degradation was accomplished in fungal cultures at initial pollutant concentrations of approximately 10 mg L{sup -1}, although a longer period of time was needed to completely remove STZ in comparison to SPY. When cytochrome P450 inhibitors were added to the fungal cultures, STZ degradation was partially suppressed, while no additional effect was observed for SPY. Experiments with purified laccase and laccase mediators caused the removal of greater than 75% of each antibiotic. Ultra-performance liquid chromatography-quadupole time of flight mass spectrometry (UPLC-QqTOF-MS) analyses allowed the identification of a total of eight degradation intermediates of SPY in both the in vivo and the laccase experiments, being its desulfonated moiety the commonly detected product. For STZ, a total of five products were identified. A fluidized bed reactor with T. versicolor pellets degraded a mixture of sulfonamides (SPY, STZ and sulfamethazine, SMZ) by greater than 94% each at a hydraulic residence time of 72 h. Because wastewater contains many diverse pollutants, these results highlight the potential of T. versicolor as a bioremediation agent not only for the removal of antibiotics but also for the elimination of a wide range of contaminants.

  11. Geographic variation in body size and its relationship with environmental gradients in the Oriental Garden Lizard, Calotes versicolor.

    Science.gov (United States)

    Wei, Xiaomei; Yan, Linmiao; Zhao, Chengjian; Zhang, Yueyun; Xu, Yongli; Cai, Bo; Jiang, Ni; Huang, Yong

    2018-05-01

    Patterns of geographic variation in body size are predicted to evolve as adaptations to local environmental gradients. However, many of these clinal patterns in body size, such as Bergmann's rule, are controversial and require further investigation into ectotherms such as reptiles on a regional scale. To examine the environmental variables (temperature, precipitation, topography and primary productivity) that shaped patterns of geographic variation in body size in the reptile Calotes versicolor , we sampled 180 adult specimens (91 males and 89 females) at 40 locations across the species range in China. The MANOVA results suggest significant sexual size dimorphism in C. versicolor ( F 23,124  = 11.32, p  body size of C. versicolor differed for males and females, but mechanisms related to heat balance and water availability hypotheses were involved in both sexes. Temperature seasonality, precipitation of the driest month, precipitation seasonality, and precipitation of the driest quarter were the most important predictors of variation in body size in males, whereas mean precipitation of the warmest quarter, mean temperature of the wettest quarter, precipitation seasonality, and precipitation of the wettest month were most important for body size variation in females. The discrepancy between patterns of association between the sexes suggested that different selection pressures may be acting in males and females.

  12. ABILITY OF Phanerochaete chrysosporium AND Trametes versicolor TO REMOVE Zn2+, Cr3+, Pb2+ METAL IONS

    Directory of Open Access Journals (Sweden)

    Josué Solís Pacheco

    2015-07-01

    Full Text Available The use of fungal biomass as an alternative for removing heavy metals has become increasingly important in recent years, replacing conventional methods based on chemical physical processes. In this study, we evaluated the biosorption of Zn2+, Cr3+ and Pb2+, which were analyzed to determine their effect on growth kinetic parameters of Phanerochaete chrysosporium strain ATCC 32629 and Trametes versicolor ATCC 1267. Growth kinetics were performed in four liquid culture media: 1 Yeast Nitrogen Base (YNB used as control, 2 YNB medium plus Pb2+ (0.25, 1 and 2 mg L-1, 3 YNB medium plus Zn2+ (5, 10 and 20 mg L-1 and 4 YNB medium plus Cr3+ (0.5, 1 and 2 mg L-1. The flasks were incubated at 25 °C with shaking at 150 rpm. Metal concentrations were determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES with prior acid digestion of the sample. The results demonstrated that Phanerochaete chrysosporium ATCC 32629 and Trametes versicolor ATCC 12679 are able to grow in the culture medium with Pb2+, Zn2+ and Cr3+ ions at different concentrations. However, P. chrysosporium ATCC 32629 showed greater adaptability and ability to adsorb Cr3+ in the culture medium at concentrations of 0.5 and 1 mg L-1, whereas T. versicolor ATCC 12679 was capable of Pb2+ biosorption at concentrations of 0.25, 1 and 2 mg L-1.

  13. Degradation of the drug sodium diclofenac by Trametes versicolor pellets and identification of some intermediates by NMR

    Energy Technology Data Exchange (ETDEWEB)

    Marco-Urrea, Ernest [Departament d' Enginyeria Quimica and Institut de Ciencia i Tecnologia Ambiental, Escola d' Enginyeria (Estonia), Universitat Autonoma de Barcelona (UAB), 08193 Bellaterra (Spain); Perez-Trujillo, Miriam [Servei de Ressonancia Magnetica Nuclear, UAB, 08193 Bellaterra (Spain); Cruz-Morato, Carles [Departament d' Enginyeria Quimica and Institut de Ciencia i Tecnologia Ambiental, Escola d' Enginyeria (Estonia), Universitat Autonoma de Barcelona (UAB), 08193 Bellaterra (Spain); Caminal, Gloria, E-mail: gloria.caminal@uab.es [Unitat de Biocatalisis Aplicada associada al IQAC (CSIC-UAB), EE, UAB, 08193 Bellaterra (Spain); Vicent, Teresa [Departament d' Enginyeria Quimica and Institut de Ciencia i Tecnologia Ambiental, Escola d' Enginyeria (Estonia), Universitat Autonoma de Barcelona (UAB), 08193 Bellaterra (Spain)

    2010-04-15

    Degradation of diclofenac sodium, a nonsteroidal anti-inflammatory drug widely found in the aquatic environment, was assessed using the white-rot fungus Trametes versicolor. Almost complete diclofenac removal ({>=}94%) occurred the first hour with T. versicolor pellets when the drug was added at relatively high (10 mg L{sup -1}) and environmentally relevant low (45 {mu}g L{sup -1}) concentrations in a defined liquid medium. In vivo and in vitro experiments using the cytochrome P450 inhibitor 1-aminobenzotriazole and purified laccase, respectively, suggested at least two different mechanisms employed by T. versicolor to initiate diclofenac degradation. Two hydroxylated metabolites, 4'-hydroxydiclofenac and 5-hydroxydiclofenac, were structurally elucidated by nuclear magnetic resonance as degradation intermediates in fungal cultures spiked with diclofenac. Both parent compound and intermediates disappeared after 24 h leading to a decrease in ecotoxicity calculated by the Microtox test. Laccase-catalyzed transformation of diclofenac led to the formation of 4-(2,6-dichlorophenylamino)-1,3-benzenedimethanol, which was not detected in in vivo experiments probably due to the low laccase activity levels observed through the first hours of incubation.

  14. Degradation of the drug sodium diclofenac by Trametes versicolor pellets and identification of some intermediates by NMR

    International Nuclear Information System (INIS)

    Marco-Urrea, Ernest; Perez-Trujillo, Miriam; Cruz-Morato, Carles; Caminal, Gloria; Vicent, Teresa

    2010-01-01

    Degradation of diclofenac sodium, a nonsteroidal anti-inflammatory drug widely found in the aquatic environment, was assessed using the white-rot fungus Trametes versicolor. Almost complete diclofenac removal (≥94%) occurred the first hour with T. versicolor pellets when the drug was added at relatively high (10 mg L -1 ) and environmentally relevant low (45 μg L -1 ) concentrations in a defined liquid medium. In vivo and in vitro experiments using the cytochrome P450 inhibitor 1-aminobenzotriazole and purified laccase, respectively, suggested at least two different mechanisms employed by T. versicolor to initiate diclofenac degradation. Two hydroxylated metabolites, 4'-hydroxydiclofenac and 5-hydroxydiclofenac, were structurally elucidated by nuclear magnetic resonance as degradation intermediates in fungal cultures spiked with diclofenac. Both parent compound and intermediates disappeared after 24 h leading to a decrease in ecotoxicity calculated by the Microtox test. Laccase-catalyzed transformation of diclofenac led to the formation of 4-(2,6-dichlorophenylamino)-1,3-benzenedimethanol, which was not detected in in vivo experiments probably due to the low laccase activity levels observed through the first hours of incubation.

  15. Adsorption of Trametes versicolor laccase to soil iron and aluminum minerals: enzyme activity, kinetics and stability studies.

    Science.gov (United States)

    Wu, Yue; Jiang, Ying; Jiao, Jiaguo; Liu, Manqiang; Hu, Feng; Griffiths, Bryan S; Li, Huixin

    2014-02-01

    Laccases play an important role in the degradation of soil phenol or phenol-like substance and can be potentially used in soil remediation through immobilization. Iron and aluminum minerals can adsorb extracellular enzymes in soil environment. In the present study, we investigated the adsorptive interaction of laccase, from the white-rot fungus Trametes versicolor, with soil iron and aluminum minerals and characterized the properties of the enzyme after adsorption to minerals. Results showed that both soil iron and aluminum minerals adsorbed great amount of laccase, independent of the mineral specific surface areas. Adsorbed laccases retained 26-64% of the activity of the free enzyme. Compared to the free laccase, all adsorbed laccases showed higher Km values and lower Vmax values, indicating a reduced enzyme-substrate affinity and a lower rate of substrate conversion in reactions catalyzed by the adsorbed laccase. Adsorbed laccases exhibited increased catalytic activities compared to the free laccase at low pH, implying the suitable application of iron and aluminum mineral-adsorbed T. versicolor laccase in soil bioremediation, especially in acid soils. In terms of the thermal profiles, adsorbed laccases showed decreased thermal stability and higher temperature sensitivity relative to the free laccase. Moreover, adsorption improved the resistance of laccase to proteolysis and extended the lifespan of laccase. Our results implied that adsorbed T. versicolor laccase on soil iron and aluminum minerals had promising potential in soil remediation. Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.

  16. Removal of pharmaceuticals, polybrominated flame retardants and UV-filters from sludge by the fungus Trametes versicolor in bioslurry reactor

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez-Rodriguez, Carlos E. [Unitat Asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 2060 San Jose (Costa Rica); Baron, Enrique; Gago-Ferrero, Pablo; Jelic, Aleksandra; Llorca, Marta; Farre, Marinella; Diaz-Cruz, M. Silvia; Eljarrat, Ethel [Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona (Spain); Petrovic, Mira [Institucio Catalana de Reserca i Estudis Avancats (ICREA), Passeig Lluis Companys 23, 80010 Barcelona (Spain); Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona (Spain); Caminal, Gloria, E-mail: Gloria.Caminal@uab.cat [Unitat Asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Barcelo, Damia [Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona (Spain); Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona (Spain); King Saud University (KSU), P.O. Box 2455, 11451 Riyadh (Saudi Arabia); and others

    2012-09-30

    Highlights: Black-Right-Pointing-Pointer Sludge from a WWTP was treated in a fungal slurry reactor with Trametes versicolor. Black-Right-Pointing-Pointer Twenty-four pharmaceuticals were removed at important extents. Black-Right-Pointing-Pointer UV-filters and brominated flame retardants were also degraded. Black-Right-Pointing-Pointer Overall toxicity of sludge increased despite the pollutant removal. - Abstract: Conventional wastewater treatments are inefficient in the removal of many organic pollutants. The presence of these contaminants in the final sludge represents a source of environmental pollution due to the increasing use of biosolids in land application. A biotechnological approach which employed the fungus Trametes versicolor in a sludge-bioslurry reactor was assessed in order to remove several groups of emerging pollutants. Biological fungal activity was monitored by means of ergosterol and laccase determinations. Fifteen out of 24 detected pharmaceuticals were removed at efficiencies over 50% after the treatment, including eight completely degraded. Removal ranged between 16-53% and 22-100% for the brominated flame retardants and the UV-filters, respectively. Only two of all the detected compounds remained unchanged after the treatment. Although elimination results are promising, the toxicity of the final sludge increased after the treatment. This finding is contrary to the toxicity results obtained in similar treatments of sludge with T. versicolor in solid-phase.

  17. Discovery of novel xylosides in co-culture of basidiomycetes Trametes versicolor and Ganoderma applanatum by integrated metabolomics and bioinformatics

    Science.gov (United States)

    Yao, Lu; Zhu, Li-Ping; Xu, Xiao-Yan; Tan, Ling-Ling; Sadilek, Martin; Fan, Huan; Hu, Bo; Shen, Xiao-Ting; Yang, Jie; Qiao, Bin; Yang, Song

    2016-09-01

    Transcriptomic analysis of cultured fungi suggests that many genes for secondary metabolite synthesis are presumably silent under standard laboratory condition. In order to investigate the expression of silent genes in symbiotic systems, 136 fungi-fungi symbiotic systems were built up by co-culturing seventeen basidiomycetes, among which the co-culture of Trametes versicolor and Ganoderma applanatum demonstrated the strongest coloration of confrontation zones. Metabolomics study of this co-culture discovered that sixty-two features were either newly synthesized or highly produced in the co-culture compared with individual cultures. Molecular network analysis highlighted a subnetwork including two novel xylosides (compounds 2 and 3). Compound 2 was further identified as N-(4-methoxyphenyl)formamide 2-O-β-D-xyloside and was revealed to have the potential to enhance the cell viability of human immortalized bronchial epithelial cell line of Beas-2B. Moreover, bioinformatics and transcriptional analysis of T. versicolor revealed a potential candidate gene (GI: 636605689) encoding xylosyltransferases for xylosylation. Additionally, 3-phenyllactic acid and orsellinic acid were detected for the first time in G. applanatum, which may be ascribed to response against T.versicolor stress. In general, the described co-culture platform provides a powerful tool to discover novel metabolites and help gain insights into the mechanism of silent gene activation in fungal defense.

  18. Removal of pharmaceuticals, polybrominated flame retardants and UV-filters from sludge by the fungus Trametes versicolor in bioslurry reactor

    International Nuclear Information System (INIS)

    Rodríguez-Rodríguez, Carlos E.; Barón, Enrique; Gago-Ferrero, Pablo; Jelić, Aleksandra; Llorca, Marta; Farré, Marinella; Díaz-Cruz, M. Silvia; Eljarrat, Ethel; Petrović, Mira; Caminal, Glòria; Barceló, Damià

    2012-01-01

    Highlights: ► Sludge from a WWTP was treated in a fungal slurry reactor with Trametes versicolor. ► Twenty-four pharmaceuticals were removed at important extents. ► UV-filters and brominated flame retardants were also degraded. ► Overall toxicity of sludge increased despite the pollutant removal. - Abstract: Conventional wastewater treatments are inefficient in the removal of many organic pollutants. The presence of these contaminants in the final sludge represents a source of environmental pollution due to the increasing use of biosolids in land application. A biotechnological approach which employed the fungus Trametes versicolor in a sludge-bioslurry reactor was assessed in order to remove several groups of emerging pollutants. Biological fungal activity was monitored by means of ergosterol and laccase determinations. Fifteen out of 24 detected pharmaceuticals were removed at efficiencies over 50% after the treatment, including eight completely degraded. Removal ranged between 16–53% and 22–100% for the brominated flame retardants and the UV-filters, respectively. Only two of all the detected compounds remained unchanged after the treatment. Although elimination results are promising, the toxicity of the final sludge increased after the treatment. This finding is contrary to the toxicity results obtained in similar treatments of sludge with T. versicolor in solid-phase.

  19. DECREASEMENT OF COLOUR AND COD CONTENT OF THE LIQUID WASTE PRODUCT FROM THE JEANS WASHING INDUSTRY BY CHITOSAN AND Trametes versicolor

    Directory of Open Access Journals (Sweden)

    Rumondang Bulan

    2010-06-01

    Full Text Available Chitosan and Trametes versicolor were used as the material for processing liquid waste product from the jeans washing industry in this study as they were more economics and no negative damage in the environmental life. This research is to find out the chitosan and Trametes versicolor effect as the coagulant in decreasing the colour and the COD content of the liquid waste product from the jeans washing industry using the coagulation method and floculation. The dosage of chitosan and Trametes versicolor were 200, 400, 600, 800 and 1000 mg/L of the liquid waste product whereas the combination of chitosan and Trametes versicolor were 4:1, 3:2, 2:3 and 1:4. The data resulted from the analysis were shown as a graphics. The research results show that the optimum dosage of chitosan as coagulant was 800 mg/L which was able to decrease the colour intensity. For blue colour, the intensity decreased from 1.2 to 0.1%; the yellow colour intensity decreased from 0.9 to 0.0% and the COD 91.23%. The optimum dosage of Trametes versicolor of 600 mg/L was able to decrease the blue colour intensity from 1.2 to 0.11%; the yellow colour intensity from 0.9 to 0.1% and the COD was 91.37%. By using the combinationof chitosan and Trametes versicolor it was shown that the best result was using the 3:2 ratio which was able to decrease the blue colour intensity from 0.9 to 0.1% and the yellow colour intensity from 0.9 to 0.0% and the COD was 86.68%.   Keywords: colour intensity, COD, liquid waste product of the jeans washing, chitosan, Trametes versicolor

  20. Plants of an Eucalyptus clone damaged by Scolytidae and Platypodidae (Coleoptera Plantas de Eucalyptus atacadas por Scolytidae e Platypodidae (Coleoptera

    Directory of Open Access Journals (Sweden)

    José Cola Zanuncio

    2005-05-01

    Full Text Available Species of Scolytidae and Platypodidae were evaluated in a plantation using a clone of Eucalyptus grandis x Eucalyptus urophylla in the State of Minas Gerais, Brazil. Wood disks with galleries were taken from 15 eucalyptus trees between 0.5 and 1.5 m height. After collection, all trees attacked were burned. Woodborers obtained from these disks were identified as Premnobius cavipennis, Premnobius ambitiosus, Dryocoetoides cristatus (Coleoptera: Scolytidae and Euplatypus parallelus (Coleoptera: Platypodidae. This is the first report of D. cristatus, a Brazilian native species, damaging eucalyptus, which shows its adaptation to this plant.O objetivo deste trabalho foi avaliar o ataque de Scolytidae e Platypodidae em talhão de clone Eucalyptus grandis x Eucalyptus urophylla na região de Montes Claros, Estado de Minas Gerais, Brasil. Discos de madeira com galerias desses insetos foram retirados de 15 árvores de eucalipto entre 0,5 e 1,5 m de altura. Após a coleta deste material, as árvores atacadas foram queimadas. Os indivíduos da ordem Coleoptera obtidos desses discos foram identificados como Premnobius cavipennis, Premnobius ambitiosus e Dryocoetoides cristatus (Coleoptera: Scolytidae e Euplatypus parallelus (Coleoptera: Platypodidae. O ataque de D. cristatus, espécie nativa do Brasil, ao eucalipto ainda não havia sido relatado, o que mostra sua adaptação a essa planta.

  1. Determination of co-metabolism for 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (DDT) degradation with enzymes from Trametes versicolor U97.

    Science.gov (United States)

    Sari, Ajeng Arum; Tachibana, Sanro; Itoh, Kazutaka

    2012-08-01

    Trametes versicolor U97 isolated from nature degraded 73% of the 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (DDT) in a malt extract liquid medium after a 40-d incubation period. This paper presents a kinetic study of microbial growth using the Monod equation. T. versicolor U97 degraded DDT during an exponential growth phase, using glucose as a carbon source for growth. The growth of T. versicolor U97 was not affected by DDT. DDT was degraded by T. versicolor U97 only when the secondary metabolism coincided with the production of several enzymes. Furthermore, modeling of several inhibitors using the partial least squares function in Minitab 15, revealed lignin peroxidase (98.7 U/l) plays a role in the degradation of DDT. T. versicolor U97 produced several metabolites included a single-ring aromatic compound, 4-chlorobenzoic acid. Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  2. Molecular markers detect cryptic predation on coffee berry borer (Coleoptera: Curculionidae) by silvanid and laemophloeid flat bark beetles (Coleoptera: Silvanidae, Laemophloeidae) in coffee beans

    Science.gov (United States)

    The coffee berry borer, Hypothenemus hampei(Coleoptera: Curculionidae)(Ferrari), is a serious pest of coffee worldwide and has been recently introduced in Hawai’i, first detected in the state in 2010. Adult silvanid flat bark beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and adult laemoph...

  3. Degradation of Aflatoxins by Means of Laccases from Trametes versicolor: An In Silico Insight

    Directory of Open Access Journals (Sweden)

    Luca Dellafiora

    2017-01-01

    Full Text Available Mycotoxins are secondary metabolites of fungi that contaminate food and feed, and are involved in a series of foodborne illnesses and disorders in humans and animals. The mitigation of mycotoxin content via enzymatic degradation is a strategy to ensure safer food and feed, and to address the forthcoming issues in view of the global trade and sustainability. Nevertheless, the search for active enzymes is still challenging and time-consuming. The in silico analysis may strongly support the research by providing the evidence-based hierarchization of enzymes for a rational design of more effective experimental trials. The present work dealt with the degradation of aflatoxin B1 and M1 by laccase enzymes from Trametes versicolor. The enzymes–substrate interaction for various enzyme isoforms was investigated through 3D molecular modeling techniques. Structural differences among the isoforms have been pinpointed, which may cause different patterns of interaction between aflatoxin B1 and M1. The possible formation of different products of degradation can be argued accordingly. Moreover, the laccase gamma isoform was identified as the most suitable for protein engineering aimed at ameliorating the substrate specificity. Overall, 3D modeling proved to be an effective analytical tool to assess the enzyme–substrate interaction and provided a solid foothold for supporting the search of degrading enzyme at the early stage.

  4. A multivariate analysis of genetic variation in the advertisement call of the gray treefrog, Hyla versicolor.

    Science.gov (United States)

    Welch, Allison M; Smith, Michael J; Gerhardt, H Carl

    2014-06-01

    Genetic variation in sexual displays is crucial for an evolutionary response to sexual selection, but can be eroded by strong selection. Identifying the magnitude and sources of additive genetic variance underlying sexually selected traits is thus an important issue in evolutionary biology. We conducted a quantitative genetics experiment with gray treefrogs (Hyla versicolor) to investigate genetic variances and covariances among features of the male advertisement call. Two energetically expensive traits showed significant genetic variation: call duration, expressed as number of pulses per call, and call rate, represented by its inverse, call period. These two properties also showed significant genetic covariance, consistent with an energetic constraint to call production. Combining the genetic variance-covariance matrix with previous estimates of directional sexual selection imposed by female preferences predicts a limited increase in call duration but no change in call rate despite significant selection on both traits. In addition to constraints imposed by the genetic covariance structure, an evolutionary response to sexual selection may also be limited by high energetic costs of long-duration calls and by preferences that act most strongly against very short-duration calls. Meanwhile, the persistence of these preferences could be explained by costs of mating with males with especially unattractive calls. © 2014 The Author(s). Evolution © 2014 The Society for the Study of Evolution.

  5. Treatment of a Textile Effluent from Dyeing with Cochineal Extracts Using Trametes versicolor Fungus

    Directory of Open Access Journals (Sweden)

    Gabriela Arroyo-Figueroa

    2011-01-01

    Full Text Available Trametes versicolor (Tv fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. However, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. Experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of Tv fungus were done. Treatments to determine decolorization in the presence or absence of Kirk's medium, glucose, and fungus, with an addition of 50% (v v-1 of nonsterilized effluent were performed. Physicochemical characterization was performed at the start and end of the treatment. Degradation kinetics were determined. A direct relationship was found between the dry weight of fungi, pH, and the decolorization system, with higher decolorization at lower pH levels (pH ~4.3. High decolorization (81% ± 0.09; 88% ± 0.17; and 99% ± 0.04 for three of the eight treatments (Kirk's medium without glucose, Kirk's medium with glucose, and without medium with glucose, respectively was found. Toxicity tests determined an increase in the initial effluent toxicity (7.33 TU compared with the final treatment (47.73 TU in a period of 11 days. For this system, a degradation sequence of the carminic acid structure present in the effluent by the Tv fungus is suggested, in which it is seen that metabolites still containing aromatic structures are generated.

  6. Flavonoid C- and O-glycosides from the Mongolian medicinal plant Dianthus versicolor Fisch.

    Science.gov (United States)

    Obmann, Astrid; Werner, Ingrid; Presser, Armin; Zehl, Martin; Swoboda, Zita; Purevsuren, Sodnomtseren; Narantuya, Samdan; Kletter, Christa; Glasl, Sabine

    2011-09-27

    Eighteen flavonoids were identified from an aqueous extract of the aerial parts of Dianthus versicolor, a plant used in traditional Mongolian medicine against liver diseases. The flavonoid C- and O-glycosides isoorientin-7-O-rutinoside, isoorientin-7-O-rhamnosyl-galactoside, isovitexin-7-O-rutinoside, isovitexin-7-O-rhamnosyl-galactoside, isoscoparin-7-O-rutinoside, isoscoparin-7-O-rhamnosyl-galactoside, isoscoparin-7-O-galactoside, and isoorientin-7-O-galactoside were isolated and structurally elucidated. Their structures were established on the basis of extensive spectroscopic techniques including LC-UV-DAD, LC-MS(n), LC-HRMS, 1D and 2D NMR spectroscopy, and by GC-MS analysis after hydrolysis. Flavonoids with such a high glycosylation pattern are rare within the genus Dianthus. Furthermore, isovitexin-7-O-glucoside (saponarin), isovitexin-2″-O-rhamnoside, apigenin-6-glucoside (isovitexin), luteolin-7-O-glucoside, apigenin-7-O-glucoside, as well as the aglycons luteolin, apigenin, chrysoeriol, diosmetin, and acacetin were identified by TLC and LC-DAD-MS(n) in comparison to reference substances or literature data. The NMR data of seven structures have not been reported in the literature to date. Copyright © 2011 Elsevier Ltd. All rights reserved.

  7. Enzyme-Catalyzed Oxidation of 17β-Estradiol Using Immobilized Laccase from Trametes versicolor

    Science.gov (United States)

    Cardinal-Watkins, Chantale; Nicell, Jim A.

    2011-01-01

    Many natural and synthetic estrogens are amenable to oxidation through the catalytic action of oxidative enzymes such as the fungal laccase Trametes versicolor. This study focused on characterizing the conversion of estradiol (E2) using laccase that had been immobilized by covalent bonding onto silica beads contained in a bench-scale continuous-flow packed bed reactor. Conversion of E2 accomplished in the reactor declined when the temperature of the system was changed from room temperature to just above freezing at pH 5 as a result of a reduced rate of reaction rather than inactivation of the enzyme. Similarly, conversion increased when the system was brought to warmer temperatures. E2 conversion increased when the pH of the influent to the immobilized laccase reactor was changed from pH 7 to pH 5, but longer-term experiments showed that the enzyme is more stable at pH 7. Results also showed that the immobilized laccase maintained its activity when treating a constant supply of aqueous E2 at a low mean residence time over a 12-hour period and when treating a constant supply of aqueous E2 at a high mean residence time over a period of 9 days. PMID:21869925

  8. Treatment of a textile effluent from dyeing with cochineal extracts using Trametes versicolor fungus.

    Science.gov (United States)

    Arroyo-Figueroa, Gabriela; Ruiz-Aguilar, Graciela M L; López-Martínez, Leticia; González-Sánchez, Guillermo; Cuevas-Rodríguez, Germán; Rodríguez-Vázquez, Refugio

    2011-05-05

    Trametes versicolor (Tv) fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. However, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. Experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of Tv fungus were done. Treatments to determine decolorization in the presence or absence of Kirk's medium, glucose, and fungus, with an addition of 50% (v v-1) of nonsterilized effluent were performed. Physicochemical characterization was performed at the start and end of the treatment. Degradation kinetics were determined. A direct relationship was found between the dry weight of fungi, pH, and the decolorization system, with higher decolorization at lower pH levels (pH ~4.3). High decolorization (81% ± 0.09; 88% ± 0.17; and 99% ± 0.04) for three of the eight treatments (Kirk's medium without glucose, Kirk's medium with glucose, and without medium with glucose, respectively) was found. Toxicity tests determined an increase in the initial effluent toxicity (7.33 TU) compared with the final treatment (47.73 TU) in a period of 11 days. For this system, a degradation sequence of the carminic acid structure present in the effluent by the Tv fungus is suggested, in which it is seen that metabolites still containing aromatic structures are generated.

  9. Phase 1 Clinical Trial of Trametes versicolor in Women with Breast Cancer.

    Science.gov (United States)

    Torkelson, Carolyn J; Sweet, Erin; Martzen, Mark R; Sasagawa, Masa; Wenner, Cynthia A; Gay, Juliette; Putiri, Amy; Standish, Leanna J

    2012-01-01

    Introduction. Orally administered preparations from the Trametes versicolor (Tv) mushroom have been hypothesized to improve immune response in women with breast cancer after standard chemotherapy and radiotherapy. Methods. A phase I, two-center, dose escalation study was done to determine the maximum tolerated dose of a Tv preparation when taken daily in divided doses for 6 weeks after recent completion of radiotherapy. Eleven participants were recruited and nine women completed the study. Each cohort was comprised of three participants given one of three doses of Tv (3, 6, or 9 grams). Immune data was collected pre- and postradiation, at 3 on-treatment time points and after a 3-week washout. Results. Nine adverse events were reported (7 mild, 1 moderate, and 1 severe), suggesting that Tv was well tolerated. Immunological results indicated trends in (1) increased lymphocyte counts at 6 and 9 grams/day; (2) increased natural killer cell functional activity at 6 grams/day; (3) dose-related increases in CD8(+) T cells and CD19(+) B cells , but not CD4(+) T cells or CD16(+)56(+) NK cells. Conclusion. These findings show that up to 9 grams/day of a Tv preparation is safe and tolerable in women with breast cancer in the postprimary treatment setting. This Tv preparation may improve immune status in immunocompromised breast cancer patients following standard primary oncologic treatment.

  10. Degradation of anthracene by laccase of Trametes versicolor in the presence of different mediator compounds.

    Science.gov (United States)

    Johannes, C; Majcherczyk, A; Hüttermann, A

    1996-10-01

    Laccase of Trametes versicolor was generally able to oxidize anthracene in vitro. After 72 h incubation about 35% of the anthracene was transformed stoichiometrically to 9,10-anthraquinone. Transformation of anthracene increased rapidly in the presence of different mediators that readily generate stable radicals: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 1-hydroxybenzotriazole. For the reaction, the presence of both the laccase and the mediator was necessary. In the presence of 0.005 mM 1-hydroxybenzotriazole this conversion had removed 47% of the anthracene after 72 h; 75% of the substrate was oxidized during this period when ABTS (1 mM) was used as mediator. In contrast to reactions without or with only low concentrations of a mediator, there was a discrepancy between the disappearance of anthracene and the formation of 9,10-anthraquinone in mediator-forced reactions. Coupling-products of mediators with anthracene degradation products were found. Anthracene disappeared nearly completely after incubation for 72 h with laccase in a 0.1 mM solution of 1-hydroxybenzotriazole and was transformed to 9,10-anthraquinone in about 80% yield; 90% of the substrate was transformed in the presence of ABTS (2.0 mM) resulting again in 80% quinone. Phenothiazine was not effective in this system.

  11. Efficient removal of disperse dye by mixed culture of ganoderma lucidum and coriolus versicolor

    International Nuclear Information System (INIS)

    Sadaf, S.; Bhatti, H.N.; Bibi, I.

    2013-01-01

    In the current study, an attempt was made to check the potential of aerobic mixed culture of two indigenous white rot fungi for the decolorization of different disperse dyes in batch culture mode and optimization of different conditions to enhance the biotransformation of dyes. Initial screening trial with six disperse dyes, viz. (Foron Yellow RD5GL, Foron Red RDRBLS, Foron Rubine RDGFL, Foron Black RD3GRN, Foron Blue RDGLN and Foron Turquoise SBLN), was carried out using mixed culture of Ganoderma lucidum and Coriolus versicolor. From all the tested dyes, the mixed culture showed better removal efficiency (93 %) with Foron Turquoise SBLN dye after 8 days of incubation period as compared to other tested dyes. Enhanced color removal (98 %) was observed when the medium was amended by ammonium tartarate, maltose, MnSO/sub 4/ at pH 4.5 and 30 degree C with 2 mL fungal culture during 2nd day of incubation period. Enzyme profile showed that the mixed culture produced three liginolytic enzymes like lignin peroxidase (LiP), manganase peroxidase (MnP) and laccase but MnP was found to be the major enzyme. The results indicated that white rot fungi (WRF) could be used to treat wastewater containing disperse dyes. (author)

  12. In vitro reduction of coplanar PCB congeners by ABTS oxidases from the culture of Trametes versicolor

    Energy Technology Data Exchange (ETDEWEB)

    Sonoki, S. [High-Tech Research Center, Azabu Univ., Kanagawa (Japan); Sue, T.; Hisamatsu, S. [Graduate School of Environmental Health, Azabu Univ., Kanagawa (Japan); Nagasaka, H. [Inst. of Environmental Ecology, Shin-Nippon Meteorological and Oceanographical Consultant Co., Ltd., Shizuoka (Japan)

    2004-09-15

    In recent years, the environmental contamination by the harmful polluted chemicals becomes more serious. Among them, especially, the dioxins such as coplanar PCBs (Co-PCBs) and PCDDs are hard to be decomposed due to their stability and hydrophobic nature, leading to the world-wide contamination. To clean up the polluted environment, bioremediation using a microorganism is expected to solve the environmental pollution problem because of cost-effective alternative to the more established engineering method. There are some reports on the biodegradation of dioxins using various organisms, in which basidiomycetes, so-called white-rot fungi, have been extensively studied in the process of lignin degradation. As a result unique extracellular oxidative lignindegrading enzymes, such as lignin peroxidase, manganese-dependent peroxidase and laccase were supposed to be responsible for degrading dioxins. Overall many studies on biodegradation of dioxins or other chlorinated aromatic hydrocarbons have focused on using white-rot fungi; however, few reports referred to the metabolism of these environmental pollutants in the in vitro reaction by use of lignin-degrading enzymes produced by white-rot fungi. In this study, we reported the reduction of levels of Co-PCBs in the in vitro incubation with the fractions which had the oxidase activity toward 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) in the culture fluid of white-rot fungus, Trametes versicolor.

  13. Functional morphology of the luminescence system of Siphamia versicolor (Perciformes: Apogonidae), a bacterially luminous coral reef fish.

    Science.gov (United States)

    Dunlap, Paul V; Nakamura, Masaru

    2011-08-01

    Previous studies of the luminescence system of Siphamia versicolor (Perciformes: Apogonidae) identified a ventral light organ, reflector, lens, duct, and a ventral diffuser extending from the throat to the caudal peduncle. The control and function of luminescence in this and other species of Siphamia, however, have not been defined. Morphological examination of fresh and preserved specimens identified additional components of the luminescence system involved in control and ventral emission of luminescence, including a retractable shutter over the ventral face of the light organ, contiguity of the ventral diffuser from the caudal peduncle to near the chin, and transparency of the bones and other tissues of the lower jaw. The shutter halves retract laterally, allowing the ventral release of light, and relax medially, blocking ventral light emission; topical application of norepinephrine to the exposed light organ resulted in retraction of the shutter halves, which suggests that operation of the shutter is under neuromuscular control. The extension of the diffuser to near the chin and transparency of the lower jaw allow a uniform emission of luminescence over the entire ventrum of the fish. The live aquarium-held fish were found to readily and consistently display ventral luminescence. At twilight, the fish left the protective association with their longspine sea urchin, Diadema setosum, and began to emit ventral luminescence and to feed on zooplankton. Ventral luminescence illuminated a zone below and around the fish, which typically swam close to the substrate. Shortly after complete darkness, the fish stopped feeding and emitting luminescence. These observations suggest that S. versicolor uses ventral luminescence to attract and feed on zooplankton from the reef benthos at twilight. Ventral luminescence may allow S. versicolor to exploit for feeding the gap at twilight in the presence of potential predators as the reef transitions from diurnally active to

  14. Morphometric analysis of instar variation in Tenebrio molitor (Coleoptera: Tenebrionidae)

    Science.gov (United States)

    Measurements of head capsule, mandible, metanotum, and body weight were done on larvae of Tenebrio molitor L. (Coleoptera: Tenebrionide) from the second to the last instar. Instar number varied from 14 to 18, but 15 or 16 instars were the most common. The value of dimensional measurements was evalua...

  15. Attractant and disruptant semiochemicals for Dendroctonus jeffreyi (Coleoptera: Curculionidae: Scolytinae)

    Science.gov (United States)

    Brian Strom; Smith S.L.; Brownie C.

    2013-01-01

    Jeffrey pine, Pinus jeffreyi Greville and Balfour, is a dominant yellow pine and important overstory component of forests growing on diverse sites from southwestern Oregon to Baja California to western Nevada. The Jeffrey pine beetle, Dedroctonus jeffreyi Hopkins (Coleoptera: Curculionidae: Scolytinae), is monophagous on Jeffrey...

  16. A coffee berry borer (Coleoptera: Curculionidae: Scolytinae) bibliography

    Science.gov (United States)

    One hundred years ago, one of the most significant biological invasions of an agricultural insect pest in the Americas was initiated. Endemic to Africa, the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae) was accidentally introduced to Brazil in 1913 and years later invaded coffe...

  17. Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera)

    NARCIS (Netherlands)

    Alexander, K.

    2003-01-01

    Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera) Data are presented on the distribution of selected species from two coleopteran families chosen to represent a random slice of the British fauna. The species have been chosen as exhibiting extremes of range

  18. Using Malaise traps to sample ground beetles (Coleoptera: Carabidae)

    Science.gov (United States)

    Michael D. Ulyshen; James L. Hanula; Scott Horn

    2005-01-01

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages...

  19. The Peritelini (Coleoptera: Curculionidae, Entiminae of the Vitale collection

    Directory of Open Access Journals (Sweden)

    Cosimo Baviera

    2016-01-01

    Full Text Available The collection of Peritelini (Coleoptera Curculionidae Entiminae currently stored in the Vitale collection of Messina University is an element of great importance for studies of taxonomy and biogeography of these rarely collected weevils. All species are commented in relation to the contributions to this taxonomic group, published on several occasions by the authors.

  20. A Comparative Study of Efficacy and Safety of Eberconazole versus Terbinafine in Patients of Tinea Versicolor.

    Science.gov (United States)

    Sharma, Jyoti; Kaushal, Jyoti; Aggarwal, Kamal

    2018-01-01

    Tinea versicolor (TV) is characterised by the appearance of maculosquamous lesions sometimes associated with mild erythema and pruritus in characteristic areas of the body. Eberconazole and terbinafine though drugs of different classes provide both mycological and clinical cure. This study aims to compare the efficacy and safety of eberconazole versus terbinafine in patients of TV. An open-label, randomised, comparative clinical trial was conducted on 60 patients. The patients were randomly divided into two study groups. Group A: Eberconazole 1% cream once daily and Group B: Terbinafine 1% cream once daily for 2 weeks. Efficacy assessment was done by observing signs and symptoms, i.e., Physician assessment 4-point scale, microscopic KOH examination, Wood's lamp examination, global clinical response assessment, and patient's assessment on visual analog scale at the end of 2 weeks and subsequently patients were reassessed at the end of 4 and 8 weeks to check any relapse. Safety assessment was also done. There was a significant improvement in all the parameters in both groups over a period of 2 weeks. Both the treatment groups, i.e., eberconazole and terbinafine were found to be safe and efficacious at the end of 2 weeks, and no statistically significant difference was observed between the two groups regarding complete cure, i.e., mycological and clinical cure (80% vs. 63.33%), respectively. However, early response (at the end of week 1) was observed with eberconazole. No relapse was seen with eberconazole, but one patient had relapse at 8 weeks with terbinafine. Both drugs had similar safety profile. Although both the drugs cured the disease, eberconazole showed better response as clinical cure and mycological cure were observed earlier and no patient relapsed in the follow-up.

  1. A comparative study of efficacy and safety of eberconazole versus terbinafine in patients of tinea versicolor

    Directory of Open Access Journals (Sweden)

    Jyoti Sharma

    2018-01-01

    Full Text Available Background: Tinea versicolor (TV is characterised by the appearance of maculosquamous lesions sometimes associated with mild erythema and pruritus in characteristic areas of the body. Eberconazole and terbinafine though drugs of different classes provide both mycological and clinical cure. Aim: This study aims to compare the efficacy and safety of eberconazole versus terbinafine in patients of TV. Materials and Methods: An open-label, randomised, comparative clinical trial was conducted on 60 patients. The patients were randomly divided into two study groups. Group A: Eberconazole 1% cream once daily and Group B: Terbinafine 1% cream once daily for 2 weeks. Efficacy assessment was done by observing signs and symptoms, i.e., Physician assessment 4-point scale, microscopic KOH examination, Wood's lamp examination, global clinical response assessment, and patient's assessment on visual analog scale at the end of 2 weeks and subsequently patients were reassessed at the end of 4 and 8 weeks to check any relapse. Safety assessment was also done. Results: There was a significant improvement in all the parameters in both groups over a period of 2 weeks. Both the treatment groups, i.e., eberconazole and terbinafine were found to be safe and efficacious at the end of 2 weeks, and no statistically significant difference was observed between the two groups regarding complete cure, i.e., mycological and clinical cure (80% vs. 63.33%, respectively. However, early response (at the end of week 1 was observed with eberconazole. No relapse was seen with eberconazole, but one patient had relapse at 8 weeks with terbinafine. Both drugs had similar safety profile. Conclusion: Although both the drugs cured the disease, eberconazole showed better response as clinical cure and mycological cure were observed earlier and no patient relapsed in the follow-up.

  2. Predation by Flat Bark Beetles (Coleoptera: Silvanidae and Laemophloeidae) on Coffee Berry Borer (Coleoptera: Curculionidae) in Hawaii coffee

    Science.gov (United States)

    Coffee berry borer(CBB), Hypothenemus hampei, is a serious pest of coffee worldwide and a new invasive pest in Hawaii. Adult flat bark beetles, mainly Leptophloeus sp.(75%) and Cathartus quadricollis(21%) (Coleoptera: Laemophloeidae and Silvanidae, respectively), were found feeding in CBB-infested c...

  3. Pitiríase versicolor: isolamento e identificação das principais espécies de Malassezia Pityriasis versicolor: isolation and identification of the main species

    Directory of Open Access Journals (Sweden)

    Valéria Maria de Souza Framil

    2010-02-01

    Full Text Available As espécies do gênero Malassezia isoladas foram: Malassezia sympodialis (16,66%, Malassezia furfur (12,50%, Malassezia globosa (11,45% e Malassezia slooffiae (2,10%. A Malassezia sympodialis foi a espécie que predominou em nosso estudo. As espécies de Malassezia identificadas não mostraram correlação com as variantes clínicas e com a distribuição das lesões de pitiríase versicolor quanto às regiões do corpo.Species of the genus Malassezia isolated were: Malassezia sympodialis (16.66%, Malassezia furfur (12.50%, Malassezia globosa (11.45%, and Malassezia slooffiae (2.10%. Malassezia sympodialis predominated in the study. The species of Malassezia identified did not show correlation with clinical variants and with the distribution of pityriasis versicolor lesions in relation to areas of the body.

  4. High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

    Directory of Open Access Journals (Sweden)

    Cristiane Ottoni

    2016-08-01

    Full Text Available Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc activity (80.2 U∙L−1 was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction. Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs.

  5. Metabolization and degradation kinetics of the urban-use pesticide fipronil by white rot fungus Trametes versicolor.

    Science.gov (United States)

    Wolfand, Jordyn M; LeFevre, Gregory H; Luthy, Richard G

    2016-10-12

    Fipronil is a recalcitrant phenylpyrazole-based pesticide used for flea/tick treatment and termite control that is distributed in urban aquatic environments via stormwater and contributes to stream toxicity. We discovered that fipronil is rapidly metabolized (t 1/2 = 4.2 d) by the white rot fungus Trametes versicolor to fipronil sulfone and multiple previously unknown fipronil transformation products, lowering fipronil concentration by 96.5%. Using an LC-QTOF-MS untargeted metabolomics approach, we identified four novel fipronil fungal transformation products: hydroxylated fipronil sulfone, glycosylated fipronil sulfone, and two compounds with unresolved structures. These results are consistent with identified enzymatic detoxification pathways wherein conjugation with sugar moieties follows initial ring functionalization (hydroxylation). The proposed pathway is supported by kinetic evidence of transformation product formation. Fipronil loss by sorption, hydrolysis, and photolysis was negligible. When T. versicolor was exposed to the cytochrome P450 enzyme inhibitor 1-aminobenzotriazole, oxidation of fipronil and production of hydroxylated and glycosylated transformation products significantly decreased (p = 0.038, 0.0037, 0.0023, respectively), indicating that fipronil is metabolized intracellularly by cytochrome P450 enzymes. Elucidating fipronil transformation products is critical because pesticide target specificity can be lost via structural alteration, broadening classes of impacted organisms. Integration of fungi in engineered natural treatment systems could be a viable strategy for pesticide removal from stormwater runoff.

  6. COPROPHANAEUS MORENOI ARNAUD, 1982 (COLEOPTERA: SCARABAEIDAE: SCARABAEINAE) IN THE GORGONA NATIONAL NATURAL PARK (COLOMBIAN PACIFIC OCEAN)

    OpenAIRE

    Cultid Medina, Carlos A; Giraldo López, Alan

    2012-01-01

    A new record of the dung beetle Coprophanaeus morenoi Arnaud, 1982 (Coleoptera: Scarabaeidae: Scarabaeinae), for the Gorgona Natural National Park (Gorgona NNP, Colombia) in the Colombian Pacific Ocean is presented. Se presenta un nuevo registro del escarabajo estercolero Coprophanaeus morenoi Arnaud, 1982 (Coleoptera: Scarabaeidae: Scarabaeinae), para el Parque Natural Nacional Gorgona, en el océano Pacifico colombiano.

  7. Het voorkomen van de glanskevers van het genus Caprophilus in Nederland (Coleoptera: Nitidulidae)

    NARCIS (Netherlands)

    Oude, de J.

    2005-01-01

    The occurrence of nitidulid beetles of the genus Carpophilus in the Netherlands (Coleoptera: Nitidulidae) Some species of the genus Carpophilus (Coleoptera: Nitidulidae) may act as pests of stored products like cocoabeans, peanuts, dried fruits like figs and dates, imported from tropical or

  8. Influence of xylem ray integrity and degree of polymerization on bending strength of beech wood decayed by Pleurotus ostreatus and Trametes versicolor

    Science.gov (United States)

    Ehsan Bari; Reza Oladi; Olaf Schmidt; Carol A. Clausen; Katie Ohno; Darrel D. Nicholas; Mehrdad Ghodskhah Daryaei; Maryam Karim

    2015-01-01

    The scope of this research was to evaluate the influence of xylem ray (XR) and degree of polymerization (DP) of holocellulose in Oriental beech wood (Fagus orientalis Lipsky.) on impact bending strength against two white-rot fungi. Beech wood specimens, exposed to Pleurotus ostreatus and Trametes versicolor, were evaluated for...

  9. The Behavioural Abilities of Meteorus versicolor Wesm. and Rogas rossicus Kok. (Hymenoptera, Branconidae) Parasitoids of Tephrina arenacearia Den. Et Schiff. (Lepidoptera, Geometridae)

    OpenAIRE

    KERIMOVA, Ilhama Gudrat Gizi

    1998-01-01

    This paper concerns with the behavioural abilities of Meteorus versicolor Wesm. and Rogas rossicus Kok. (Hymenoptera, Braconidae)-internal parasitoids of Thephrina arenacearia Den. et Schiff. (Lepidoptera, Geometridae) as well. Periods of development phases and searching, ovipositional and mating behaviour of both parasitoids have been investigated.

  10. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor

    NARCIS (Netherlands)

    Kozarski, M.; Klaus, A.; Niksic, M.; Vrvic, M.M.; Todorovic, N.; Jakovljevic, D.; Griensven, van L.J.L.D.

    2012-01-01

    Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts

  11. Effects of onion (Allium cepa L.) and garlic (Allium sativum L.) essential oils on the Aspergillus versicolor growth and sterigmatocystin production.

    Science.gov (United States)

    Kocić-Tanackov, Sunčica; Dimić, Gordana; Lević, Jelena; Tanackov, Ilija; Tepić, Aleksandra; Vujičić, Biserka; Gvozdanović-Varga, Jelica

    2012-05-01

    In the present study the effects of individual and combined essential oils (EOs) extracted from onion (Allium cepa L.) bulb and garlic (Allium sativum L.) clove on the growth of Aspergillus versicolor and sterigmatocystin (STC) production were investigated. The EOs obtained by hydrodistillation were analyzed by GC/MS. Twenty one compounds were identified in onion EO. The major components were: dimethyl-trisulfide (16.64%), methyl-propyl-trisulfide (14.21%), dietil-1,2,4-tritiolan (3R,5S-, 3S,5S- and 3R,5R- isomers) (13.71%), methyl-(1-propenyl)-disulfide (13.14%), and methyl-(1-propenyl)-trisulfide (13.02%). The major components of garlic EO were diallyl-trisulfide (33.55%), and diallyl-disulfide (28.05%). The mycelial growth and the STC production were recorded after 7, 14, and 21 d of the A. versicolor growth in Yeast extract sucrose (YES) broth containing different EOs concentrations. Compared to the garlic EO, the onion EO showed a stronger inhibitory effect on the A. versicolor mycelial growth and STC production. After a 21-d incubation of fungi 0.05 and 0.11 μg/mL of onion EO and 0.11 μg/mL of garlic EO completely inhibited the A. versicolor mycelial growth and mycotoxins biosynthesis. The combination of EOs of onion (75%) and garlic (25%) had a synergistic effect on growth inhibition of A. versicolor and STC production. © 2012 Institute of Food Technologists®

  12. Comparison of two laccases from Trametes versicolor for application in the decolorization of dyes.

    Science.gov (United States)

    Li, Qi; Ge, Lin; Cai, Junli; Pei, Jianjun; Xie, Jingcong; Zhao, Linguo

    2014-04-01

    It has been previously demonstrated that laccases exhibit great potential for use in several industrial and environmental applications. In this paper, two laccase isoenzyme genes, lccB and lccC, were cloned and expressed in Pichia pastoris GS115. The sequence analysis indicated that the lccB and lccC genes consisted of 1,563 and 1,584 bp, and their open reading frames encoded 520 and 527 amino acids, respectively. They had 72.7% degree of identity in nucleotides and 86.7% in amino acids. The expression levels of LccB and LccC were up to 32,479 and 34,231 U/l, respectively. The recombinant laccases were purified by ultrafiltration and (NH4)2SO4 precipitation, showing a single band on SDS-PAGE, which had a molecular mass of 58 kDa. The optimal pH and temperature for LccB were 2.0 and 55°C with 2,2'-azino-bis-[3-ethylbenzthiazolinesulfonic acid (ABTS) as a substrate, whereas LccC exhibited optimal pH and temperature at 3.0 and 60°C. The apparent kinetic parameters of LccB were 0.43 mM for ABTS with a Vmax value of 51.28 U/mg, and the Km and Vmax values for LccC were 0.29 mM and 62.89 U/mg. The recombinant laccases were able to decolorize five types of dyes. Acid Violet 43 (100 g/ml) was completely decolorized by LccB or LccC (2 U/ml), and the decolorization of Reactive Blue KN-R (100 g/ml) was 91.6% by LccC (2 U/ml). Thus, the study characterizes useful laccase isoenzymes from T. versicolor that have the capability of being incorporated into the treatment of similar azo and anthraquinone dyes from dyeing industries.

  13. Plant compounds insecticide activity against Coleoptera pests of stored products

    OpenAIRE

    MOREIRA, M.D.; PICANÇO, M.C.; BARBOSA, L.C. de A.; GUEDES, R.N.C.; CAMPOS, M.R. de; SILVA, G.A.; MARTINS, J.C.

    2008-01-01

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed...

  14. Sex- and Size-Related Patterns of Carrion Visitation in Necrodes littoralis (Coleoptera: Silphidae) and Creophilus maxillosus (Coleoptera: Staphylinidae).

    Science.gov (United States)

    Mądra-Bielewicz, Anna; Frątczak-Łagiewska, Katarzyna; Matuszewski, Szymon

    2017-09-01

    The estimation of postmortem interval (PMI) based on successional patterns of adult insects is largely limited, due to the lack of potential PMI markers. Sex and size of adult insects could be easily used for such estimation. In this study, sex- and size-related patterns of carrion attendance by adult insects were analyzed in Necrodes littoralis (Coleoptera: Silphidae) and Creophilus maxillosus (Coleoptera: Staphylinidae). For both species, abundance of males and females changed similarly during decomposition. A slightly female-biased sex ratio was recorded in N. littoralis. Females of N. littoralis started visiting carcasses, on average, one day earlier than males. There was a rise in size of males of N. littoralis at the end of decomposition, whereas for females of both species and males of C. maxillosus, no size-related patterns of carrion visitation were found. Current results demonstrate that size and sex of adult carrion beetles are poor indicators of PMI. © 2016 American Academy of Forensic Sciences.

  15. Estados inmaduros de Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini Immature stages of Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini

    Directory of Open Access Journals (Sweden)

    Jhon César Neita-Moreno

    2008-12-01

    Full Text Available Se describen y se ilustran por primera vez la larva de tercer estadio y la pupa de Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini. Se aportan datos sobre la biología de la especie y su distribución en Colombia. Se proporciona una clave para la identificación de las larvas de tercer estadio conocidas de las especies del género Ancognatha Erichson.The third instar larva and pupa of Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini are described and illustrated for the first time. New data on larval biology and distribution of the species in Colombia are included. A key to the known third stage larvae of Ancognatha Erichson is provided.

  16. Intoxicação experimental por Simarouba versicolor (Simaroubaceae em ovinos e indução de resistência ao consumo da planta

    Directory of Open Access Journals (Sweden)

    Ariany C. Santos

    2013-03-01

    Full Text Available Simarouba versicolor é uma árvore semidecídua pertencente à família Simaroubaceae. Um surto de intoxicação por S. versicolor em bovinos por brotos da planta presente no pasto em Mato Grosso do Sul e sua reprodução experimental foram descritos. Esse estudo teve por objetivos verificar experimentalmente se os ovinos podem ser utilizados como modelo clínico-patológico no estudo da intoxicação por Simarouba versicolor St. Hil. (fam. Simaroubaceae, determinar se há indução de resistência pela ingestão de pequenas e repetidas doses e, se a planta mantém sua toxicidade quando dessecada. Foram realizados dois experimentos, sendo o primeiro com folhas verdes ou folhas dessecadas e trituradas de S. versicolor em doses únicas de 5g/kg, 5g/kg e 3g/kg a três ovinos (Ovino 1, 2 e 3 respectivamente. O experimento 2, foi realizado com diferentes doses diárias de folhas dessecadas e trituradas de S. versicolor em quatro ovinos que receberam 1,5g/kg, 0,75g/kg, 0,6g/kg e 0,3g/kg e, com um ovino que recebeu 3g/kg como controle positivo (Ovino 4. A administração foi suspensa quando os ovinos apresentaram sinais clínicos da intoxicação. Após doze dias de recuperação, os animais sobreviventes foram desafiados com a mesma dose diária da planta ingerida anteriormente para avaliar o desenvolvimento de resistência. Os sinais clínicos observados nos dois experimentos caracterizaram-se por anorexia, mucosas oculares congestas, polidipsia, sialorreia, fezes pastosas que evoluíram para diarreia líquida fétida esverdeada, decúbito lateral e morte para os Ovinos 1 a 7. As principais lesões histológicas observadas foram necrose do tecido linfoide (linfonodos, baço, placas de Peyer e enterite necrosante. Com os resultados obtidos, pode-se concluir que os ovinos podem ser utilizados como modelo experimental clínico-patológico na intoxicação por S. versicolor. Com o método utilizado, não houve resistência ao consumo diário de folhas

  17. Extracts from the Mongolian traditional medicinal plants Dianthus versicolorFisch. and Lilium pumilum Delile stimulate bile flow in an isolated perfused rat liver model.

    Science.gov (United States)

    Obmann, Astrid; Tsendayush, Damba; Thalhammer, Theresia; Zehl, Martin; Vo, Thanh Phuong Nha; Purevsuren, Sodnomtseren; Natsagdorj, Damdinsuren; Narantuya, Samdan; Kletter, Christa; Glasl, Sabine

    2010-10-05

    Dianthus versicolor (Caryophyllaceae) and Lilium pumilum (Liliaceae) are two medicinal plants used in traditional Mongolian medicine to treat hepatic and gastrointestinal disorders. In this study aqueous (AE) and methanolic (ME) extracts of Dianthus versicolor and Lilium pumilum were investigated for their influence on the bile flow. The aqueous extracts of both plants were tested in absence and presence of 10 μM taurocholic acid at three different concentrations (100, 250, and 500 mg/L). The aqueous extract of Dianthus versicolor was further purified in order to locate the active principles. Two resulting fractions, one enriched in flavonoids and the other in sugars, were investigated for their influence on the bile flow in absence of taurocholic acid at 10, 20, and 40 mg/L. The aqueous extracts of both plants were analysed qualitatively by LC-MS(n) and quantitatively by UV-spectrophotometry. The bile flow experiments were performed in the isolated perfused rat liver. The compounds were identified by LC-DAD-MS(n) and TLC using references. The UV-spectrophotometric analysis was based on the monograph "Passiflorae herba" of the European Pharmacopoeia, and the total flavonoid contents were calculated and expressed as vitexin. AE and ME of both plants increased the bile flow dose-dependently (between 9% and 30%), and no hepatotoxic effect was seen even during longer perfusions. Stimulation of bile secretion was comparable in the presence and in the absence of taurocholic acid. The flavonoid fraction of Dianthus versicolor increased the bile flow by 18% (pDianthus versicolor AE (total flavonoid content 1.78%) revealed the presence of the isovitexin derivative saponarin. In the AE of Lilium pumilum (total flavonoid content 1.04%) the flavonoids rutoside, kaempferol-3-O-rutinoside, and isorhamnetin-3-O-rutinoside were detected. The results show that choleresis under extract application is due to a stimulation of the bile-salt-independent bile flow which might be caused

  18. Metabolomics Investigation of an Association of Induced Features and Corresponding Fungus during the Co-culture of Trametes versicolor and Ganoderma applanatum

    Science.gov (United States)

    Xu, Xiao-Yan; Shen, Xiao-Ting; Yuan, Xiao-Jie; Zhou, Yuan-Ming; Fan, Huan; Zhu, Li-Ping; Du, Feng-Yu; Sadilek, Martin; Yang, Jie; Qiao, Bin; Yang, Song

    2018-01-01

    The co-culture of Trametes versicolor and Ganoderma applanatum is a model of intense basidiomycete interaction, which induces many newly synthesized or highly produced features. Currently, one of the major challenges is an identification of the origin of induced features during the co-culture. Herein, we report a 13C-dynamic labeling analysis used to determine an association of induced features and corresponding fungus even if the identities of metabolites were not available or almost nothing was known of biochemical aspects. After the co-culture of T. versicolor and G. applanatum for 10 days, the mycelium pellets of T. versicolor and G. applanatum were sterilely harvested and then mono-cultured in the liquid medium containing half fresh medium with 13C-labeled glucose as carbon source and half co-cultured supernatants collected on day 10. 13C-labeled metabolome analyzed by LC-MS revealed that 31 induced features including 3-phenyllactic acid and orsellinic acid were isotopically labeled in the mono-culture after the co-culture stimulation. Twenty features were derived from T. versicolor, 6 from G. applanatum, and 5 features were synthesized by both T. versicolor and G. applanatum. 13C-labeling further suggested that 12 features such as previously identified novel xyloside [N-(4-methoxyphenyl)formamide 2-O-beta-D-xyloside] were likely induced through the direct physical interaction of mycelia. Use of molecular network analysis combined with 13C-labeling provided an insight into the link between the generation of structural analogs and producing fungus. Compound 1 with m/z 309.0757, increased 15.4-fold in the co-culture and observed 13C incorporation in the mono-culture of both T. versicolor and G. applanatum, was purified and identified as a phenyl polyketide, 2,5,6-trihydroxy-4, 6-diphenylcyclohex-4-ene-1,3-dione. The biological activity study indicated that this compound has a potential to inhibit cell viability of leukemic cell line U937. The current work sets

  19. HPLC determination of flavonoid glycosides in Mongolian Dianthus versicolor Fisch. (Caryophyllaceae) compared with quantification by UV spectrophotometry.

    Science.gov (United States)

    Obmann, Astrid; Purevsuren, Sodnomtseren; Zehl, Martin; Kletter, Christa; Reznicek, Gottfried; Narantuya, Samdan; Glasl, Sabine

    2012-01-01

    Dianthus versicolor is used in traditional Mongolian medicine against liver impairment. Fractions enriched in flavone-di- and triglycosides were shown to enhance bile secretion. Therefore, reliable and accurate analytical methods are needed for the determination of these flavonoids in the crude drug and extracts thereof. To provide a validated HPLC-DAD (diode array detector) method especially developed for the separation of polar flavonoids and to compare the data obtained with those evaluated by UV spectrophotometry. Separations were carried out on an Aquasil® C₁₈-column (4.6 mm × 250.0 mm, 5 µm) with a linear gradient of acetonitrile and water (adjusted to pH 2.8 with trifluoroacetic acid) as mobile phase. Rutoside was employed as internal standard with linear behavior in a concentration range of 0.007-3.5 mg/mL. Accuracy was determined by spiking the crude drug with saponarin resulting in recoveries between 92% and 102%. The method allows the quantification of highly polar flavonoid glycosides and the determination of their total content. For saponarin a linear response was evaluated within the range 0.007-3.5 mg/mL (R²  > 0.9999). It was proven that threefold sonication represents a time-saving, effective and cheap method for the extraction of the polar flavonoid glycosides. The contents determined by HPLC were shown to be in agreement with those obtained employing UV spectrophotometry. The study has indicated that the newly developed HPLC method represents a powerful technique for the quality control of D. versicolor. Ultraviolet spectrophotometry may be used alternatively provided that the less polar flavonoids are removed by purification. Copyright © 2011 John Wiley & Sons, Ltd.

  20. Enzymatic removal of estrogenic activity of nonylphenol and octylphenol aqueous solutions by immobilized laccase from Trametes versicolor

    Energy Technology Data Exchange (ETDEWEB)

    Catapane, Maria [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Nicolucci, Carla; Menale, Ciro; Mita, Luigi [National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138 Naples (Italy); Rossi, Sergio [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); Mita, Damiano G., E-mail: mita@igb.cnr.it [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138 Naples (Italy); Diano, Nadia [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138 Naples (Italy)

    2013-03-15

    Highlights: ► Endocrine disruptors cause adverse effects in living organisms. ► Nonylphenol and Octylphenol are alkylphenols recognized as endocrine disruptors. ► It is necessary to remove or reduce their presence in the environment. ► Waters polluted by these pollutants have been bioremediated by immobilized laccase from Trametes versicolor. ► Laccase treated solutions were found to have lost any estrogenic activity. -- Abstract: A fluidized bed reactor, filled with laccase-based beads, has been employed to bioremediate aqueous solutions polluted by endocrine disruptors belonging to the alkylphenols (APs) class. In particular Octylphenol and Nonylphenol have been studied. The catalytic activity of free and immobilized laccase from Trametes versicolor has been characterized as a function of pH, temperature and substrate concentration in the reaction medium. In view of practical applications for each substrate concentration the removal efficiency (RE), the time to halve the initial concentration (τ{sub 50}), and the t{sub c=0}, i.e. the time to reach complete pollutant removal, have been calculated. The immobilized laccase exhibited a lower affinity for octylphenol (K{sub m} = 1.11 mM) than for Nonylphenol (K{sub m} = 0.72 mM), but all the other parameters of applicative interest resulted more significant for octylphenol. For example, the times to reach the complete removal of octylphenol compared to those for nonylphenol at the same concentration is shorter of about 15% (at low concentrations) up to 40% (at high concentrations). The study of cell proliferation with MPP89 cells, a human mesothelioma cell line, and the assay with the YES test indicated the loss of estrogenic activity of the APs solutions after laccase treatment.

  1. Family-group names in Coleoptera (Insecta)

    Science.gov (United States)

    Bouchard, Patrice; Bousquet, Yves; Davies, Anthony E.; Alonso-Zarazaga, Miguel A.; Lawrence, John F.; Lyal, Chris H. C.; Newton, Alfred F.; Reid, Chris A. M.; Schmitt, Michael; Ślipiński, S. Adam; Smith, Andrew B. T.

    2011-01-01

    Abstract We synthesize data on all known extant and fossil Coleoptera family-group names for the first time. A catalogue of 4887 family-group names (124 fossil, 4763 extant) based on 4707 distinct genera in Coleoptera is given. A total of 4492 names are available, 183 of which are permanently invalid because they are based on a preoccupied or a suppressed type genus. Names are listed in a classification framework. We recognize as valid 24 superfamilies, 211 families, 541 subfamilies, 1663 tribes and 740 subtribes. For each name, the original spelling, author, year of publication, page number, correct stem and type genus are included. The original spelling and availability of each name were checked from primary literature. A list of necessary changes due to Priority and Homonymy problems, and actions taken, is given. Current usage of names was conserved, whenever possible, to promote stability of the classification. New synonymies (family-group names followed by genus-group names): Agronomina Gistel, 1848 syn. nov. of Amarina Zimmermann, 1832 (Carabidae), Hylepnigalioini Gistel, 1856 syn. nov. of Melandryini Leach, 1815 (Melandryidae), Polycystophoridae Gistel, 1856 syn. nov. of Malachiinae Fleming, 1821 (Melyridae), Sclerasteinae Gistel, 1856 syn. nov. of Ptilininae Shuckard, 1839 (Ptinidae), Phloeonomini Ádám, 2001 syn. nov. of Omaliini MacLeay, 1825 (Staphylinidae), Sepedophilini Ádám, 2001 syn. nov. of Tachyporini MacLeay, 1825 (Staphylinidae), Phibalini Gistel, 1856 syn. nov. of Cteniopodini Solier, 1835 (Tenebrionidae); Agronoma Gistel 1848 (type species Carabus familiaris Duftschmid, 1812, designated herein) syn. nov. of Amara Bonelli, 1810 (Carabidae), Hylepnigalio Gistel, 1856 (type species Chrysomela caraboides Linnaeus, 1760, by monotypy) syn. nov. of Melandrya Fabricius, 1801 (Melandryidae), Polycystophorus Gistel, 1856 (type species Cantharis aeneus Linnaeus, 1758, designated herein) syn. nov. of Malachius Fabricius, 1775 (Melyridae), Sclerastes

  2. Family-Group Names In Coleoptera (Insecta

    Directory of Open Access Journals (Sweden)

    Patrice Bouchard

    2011-04-01

    Full Text Available We synthesize data on all known extant and fossil Coleoptera family-group names for the first time. A catalogue of 4887 family-group names (124 fossil, 4763 extant based on 4707 distinct genera in Coleoptera is given. A total of 4492 names are available, 183 of which are permanently invalid because they are based on a preoccupied or a suppressed type genus. Names are listed in a classification framework. We recognize as valid 24 superfamilies, 211 families, 541 subfamilies, 1663 tribes and 740 subtribes. For each name, the original spelling, author, year of publication, page number, correct stem and type genus are included. The original spelling and availability of each name were checked from primary literature. A list of necessary changes due to Priority and Homonymy problems, and actions taken, is given. Current usage of names was conserved, whenever possible, to promote stability of the classification. New synonymies (family-group names followed by genus-group names: Agronomina Gistel, 1848 syn. n. of Amarina Zimmermann, 1832 (Carabidae, Hylepnigalioini Gistel, 1856 syn. n. of Melandryini Leach, 1815 (Melandryidae, Polycystophoridae Gistel, 1856 syn. n. of Malachiinae Fleming, 1821 (Melyridae, Sclerasteinae Gistel, 1856 syn. n. of Ptilininae Shuckard, 1839 (Ptinidae, Phloeonomini Ádám, 2001 syn. n. of Omaliini MacLeay, 1825 (Staphylinidae, Sepedophilini Ádám, 2001 syn. n. of Tachyporini MacLeay, 1825 (Staphylinidae, Phibalini Gistel, 1856 syn. n. of Cteniopodini Solier, 1835 (Tenebrionidae; Agronoma Gistel 1848 (type species Carabus familiaris Duftschmid, 1812, designated herein syn. n. of Amara Bonelli, 1810 (Carabidae, Hylepnigalio Gistel, 1856 (type species Chrysomela caraboides Linnaeus, 1760, by monotypy syn. n. of Melandrya Fabricius, 1801 (Melandryidae, Polycystophorus Gistel, 1856 (type species Cantharis aeneus Linnaeus, 1758, designated herein syn. n. of Malachius Fabricius, 1775 (Melyridae, Sclerastes Gistel, 1856 (type species

  3. DNA Damage and DNA Damage Responses in THP-1 Monocytes after Exposure to Spores of either Stachybotrys chartarum or Aspergillus versicolor or to T-2 toxin

    OpenAIRE

    Rakkestad, Kirsten E.; Skaar, Ida; Ansteinsson, Vibeke E.; Solhaug, Anita; Holme, Jørn A.; Pestka, James J.; Samuelsen, Jan T.; Dahlman, Hans J.; Hongslo, Jan K.; Becher, Rune

    2010-01-01

    We have characterized cell death in THP-1 cells after exposure to heat-treated spores from satratoxin G–producing Stachybotrys chartarum isolate IBT 9631, atranone-producing S. chartarum isolate IBT 9634, and sterigmatocystin-producing Aspergillus versicolor isolate IBT 3781, as well as the trichothecenes T-2 and satratoxin G. Spores induced cell death within 3–6 h, with Stachybotrys appearing most potent. IBT 9631 induced both apoptosis and necrosis, while IBT 9634 and IBT 3781 induced mostl...

  4. A Novel Contrast Stain for the Rapid Diagnosis of Pityriasis Versicolor: A Comparison of Chicago Sky Blue 6B Stain, Potassium Hydroxide Mount and Culture.

    Science.gov (United States)

    Lodha, Nikita; Poojary, Shital Amin

    2015-01-01

    The mycological study of pityriasis versicolor is usually done by potassium hydroxide (KOH) mount and culture. However, KOH mount lacks a color contrast and requires a trained eye to interpret, while culture is difficult to perform, time consuming and has low sensitivity. Chicago Sky Blue 6B (CSB) is a new contrast stain that highlights the fungal hyphae and spores, blue against a purplish background. This study was done to compare the utility of a novel contrast stain (CSB stain) with KOH mount and culture. Skin scrapings from the lesions of 100 clinically diagnosed cases of P. versicolor were subjected to (1) KOH mount and CSB stain for direct microscopic examination and (2) culture using Sabouraud's dextrose agar. The statistical analysis of CSB stain and culture was done using KOH mount as the reference method, as it is the most commonly performed and practical diagnostic test available for P. versicolor. An interrater reliability analysis using the Cohen's Kappa statistic was performed to determine consistency (agreement) among the different modalities. Direct microscopy with CSB stain, KOH mount and mycological culture showed positive results in 98 (98%), 92 (92%) and 56 (56%) patients, respectively. Using KOH mount as the reference method, CSB stain had a sensitivity of 100% which was significantly higher than culture (60.9%). Statistically significant fair agreement was found between CSB stain and KOH mount (94% with κ=0.38, P < 0.001). Negligible agreement was found between CSB stain and culture (66%, κ=0.199, P = 0.001) as well as between KOH mount and culture (64%, κ=0.051, P = 0.107). CSB staining of skin scrapings is the most sensitive method for the diagnosis of pityriasis versicolor. Due to the distinct contrast provided by CSB, it is easy to perform, rapid and qualitatively superior to KOH mount.

  5. A novel contrast stain for the rapid diagnosis of pityriasis versicolor: A comparison of Chicago Sky Blue 6B stain, potassium hydroxide mount and culture

    Directory of Open Access Journals (Sweden)

    Nikita Lodha

    2015-01-01

    Full Text Available Background: The mycological study of pityriasis versicolor is usually done by potassium hydroxide (KOH mount and culture. However, KOH mount lacks a color contrast and requires a trained eye to interpret, while culture is difficult to perform, time consuming and has low sensitivity. Chicago Sky Blue 6B (CSB is a new contrast stain that highlights the fungal hyphae and spores, blue against a purplish background. Aims and Objectives: This study was done to compare the utility of a novel contrast stain (CSB stain with KOH mount and culture. Materials and Methods: Skin scrapings from the lesions of 100 clinically diagnosed cases of P. versicolor were subjected to (1 KOH mount and CSB stain for direct microscopic examination and (2 culture using Sabouraud′s dextrose agar. The statistical analysis of CSB stain and culture was done using KOH mount as the reference method, as it is the most commonly performed and practical diagnostic test available for P. versicolor. An interrater reliability analysis using the Cohen′s Kappa statistic was performed to determine consistency (agreement among the different modalities. Observations and Results: Direct microscopy with CSB stain, KOH mount and mycological culture showed positive results in 98 (98%, 92 (92% and 56 (56% patients, respectively. Using KOH mount as the reference method, CSB stain had a sensitivity of 100% which was significantly higher than culture (60.9%. Statistically significant fair agreement was found between CSB stain and KOH mount (94% with κ=0.38, P < 0.001. Negligible agreement was found between CSB stain and culture (66%, κ=0.199, P = 0.001 as well as between KOH mount and culture (64%, κ=0.051, P = 0.107. Conclusion: CSB staining of skin scrapings is the most sensitive method for the diagnosis of pityriasis versicolor. Due to the distinct contrast provided by CSB, it is easy to perform, rapid and qualitatively superior to KOH mount.

  6. Biodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC-QqTOF-MS

    Energy Technology Data Exchange (ETDEWEB)

    Garcia-Galan, Ma. Jesus, E-mail: mggqam@cid.csic.es [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona (Spain); Rodriguez-Rodriguez, Carlos E., E-mail: CarlosEsteban.Rodriguez@uab.cat [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 2060 San Jose (Costa Rica); Vicent, Teresa, E-mail: Teresa.Vicent@uab.cat [Departament d' Enginyeria Quimica, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Caminal, Gloria, E-mail: Gloria.Caminal@uab.cat [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Diaz-Cruz, M. Silvia, E-mail: sdcqam@cid.csic.es [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona (Spain); Barcelo, Damia, E-mail: dbcqam@cid.csic.es [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona (Spain); Catalan Institute for Water Research (ICRA), Parc Cientific i Tecnologic de la Universitat de Girona. C/Emili Grahit, 101 Edifici H2O, E-17003 Girona (Spain); King Saud University, P.O. Box 2455, Riyadh 11451 (Saudi Arabia)

    2011-11-15

    Degradation of the sulfonamide sulfamethazine (SMZ) by the white-rot fungus Trametes versicolor was assessed. Elimination was achieved to nearly undetectable levels after 20 h in liquid medium when SMZ was added at 9 mg L{sup -1}. Experiments with purified laccase and laccase-mediators resulted in almost complete removal. On the other hand, inhibition of SMZ degradation was observed when piperonilbutoxide, a cytochrome P450-inhibitor, was added to the fungal cultures. UPLC-QqTOF-MS analysis allowed the identification and confirmation of 4 different SMZ degradation intermediates produced by fungal cultures or purified laccase: desulfo-SMZ, N{sup 4}-formyl-SMZ, N{sup 4}-hydroxy-SMZ and desamino-SMZ; nonetheless SMZ mineralization was not demonstrated with the isotopically labeled sulfamethazine-phenyl-{sup 13}C{sub 6} after 7 days. Inoculation of T. versicolor to sterilized sewage sludge in solid-phase systems showed complete elimination of SMZ and also of other sulfonamides (sulfapyridine, sulfathiazole) at real environmental concentrations, making this fungus an interesting candidate for further remediation research. - Highlights: {yields}Degradation of sulfamethazine by Trametes versicolor was evaluated. {yields}The laccase enzymatic system and cytochrome P-450 were involved in the degradation. {yields}Four different degradation products of sulfamethazine were identified and confirmed. {yields}The molecular structures and masses of the metabolites were accurately calculated. {yields}Full elimination of sulfamethazine was observed in regular sewage sludge.

  7. Scymnus camptodromus (Coleoptera: Coccinellidae) larval development and predation of hemlock woolly adelgid (Hemiptera: Adelgidae)

    Science.gov (United States)

    Samita Limbu; Melody A. Keena; David Long; Nancy Ostiguy; Kelli. Hoover

    2015-01-01

    Development time and prey consumption of Scymnus (Neopullus) camptodromus Yu and Liu (Coleoptera: Coccinellidae) larvae by instar, strain, and temperature were evaluated. S. camptodromus, a specialist predator of hemlock woolly adelgid Adelges tsugae (Annand) (Hemiptera:...

  8. First record of Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae in Brazil

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2015-03-01

    Full Text Available Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae is recorded for the first time in Brazil (Bahia. It was originally described from Europe and is currently widely distributed in that continent and Asia.

  9. Aggregation pheromone compounds of the black larder beetle Dermestes haemorrhoidalis Kuster (Coleoptera: Dermestidae)

    NARCIS (Netherlands)

    Korada, R.R.; Griepink, F.C.

    2011-01-01

    Gas chromatography with simultaneous flame ionisation and electroantennographic detection (GCEAD) and gas chromatography with mass spectrometry analysis (GCMS) of abdominal extracts of adult male Dermestes haemorrhoidalis Kuster (Coleoptera: Dermestidae) revealed the presence of

  10. A predictive distribution model for Graphoderus bilineatus in the Netherlands (Coleoptera: Dytiscidae).

    NARCIS (Netherlands)

    Sierdsema, H.; Cuppen, J.G.M.

    2006-01-01

    Een voorspellend verspreidingsmodel voor de gestreepte waterroofkever Graphoderus bilineatus in Nederland (Coleoptera: Dytiscidae) Op verzoek van de Provincie Zuid-Holland en het Ministerie van lnv is het huidige voorkomen en de biotoopvoorkeur van de gestreepte waterroofkever Graphoderus bilineatus

  11. Illustrated guide to the emerald ash borer, Agrilus planipennis Fairmaire and related species (Coleoptera, Buprestidae)

    Science.gov (United States)

    The 33 species of Agrilus (Coleoptera: Buprestidae) hypothesized to be most closely related to Agrilus planipennis Fairmaire (the emerald ash borer), are described and illustrated. Morphology (adults and immatures), biology, distribution, detailed taxonomic history and systematics are presented fo...

  12. Two new fossil species of Cryptocephalus Geoffroy (Coleoptera: Chrysomelidae) from Baltic and Dominican Amber

    Science.gov (United States)

    Two new species of Cryptocephalus Geoffroy (Coleoptera: Chrysomelidae) are described and illustrated from fossil resin: Cryptocephalus groehni sp. nov (Baltic amber) and Cryptocephalus kheelorum sp. nov. (Dominican amber). These are the first described species of Cryptocephalinae from fossil resin. ...

  13. Primer registro de Hippodamia variegata (Goeze 1777) (Coleoptera: Coccinellidae) en el Perú.

    OpenAIRE

    Bustamante-Navarrete, Abdhiel; Marquina-Montesinos, Edgar Luis; Elme-Tumpay, Araseli

    2017-01-01

    First report of Hippodamia variegata (Goeze 1777) (Coleoptera: Coccinellidae) in Peru. The first record of Hippodamia variegata (Goeze 1777) (Coleoptera: Coccinellidae) in Peru is reported, which also constitutes the highest record of the species. This introduced species is already established in the rest of the continent, but its presence in Peru is confirmed in this contribution. A distribution map and a key to distinguish the two species of this genus present in Peru are presented.

  14. Biologi Hama Kumbang Penggerek Pucuk Kelapa Sawit (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) Di Rumah Kassa

    OpenAIRE

    Sejahtra, Ahmad

    2011-01-01

    Ahmad Sejahtra, "BIOLOGY SHOOT BORERS BEETLE PEST OF COCONUT PALM (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) AT KASSA HOUSE", Under supervised by Ms Marheni and Ms Fatima Zahara. This study aims to determine biological pest of oil palm bud weevil (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) at home kassa. The experiment was conducted on people's plantations in PERUMNAS Simalingkar starting from September 2010 until April 2011. Research using observational methods for this resear...

  15. The tribe Phanaeini (Coleoptera: Scarabaeidae: Scarabaeinae in Peru

    Directory of Open Access Journals (Sweden)

    Luis Figueroa

    2014-10-01

    Full Text Available We examine the occurrence of the tribe Phanaeini (Coleoptera: Scarabaeidae: Scarabaeinae in Peru based on the collection at Museo de Historia Natural of the Universidad Nacional Mayor de San Marcos and on data provided in literature. Each species is presented with citations of its diagnosis, distribution and related comments. Peruvian Phanaeini includes 30 species in nine genera: Coprophanaeus, Dendropaemon, Gromphas, Oruscatus, Oxysternon, Phanaeus, Sulcophanaeus, Tetramereia and Megatharsis. Oruscatus davus is the only species distributed in the high Andes; Phanaeus lunaris and P. achilles occur in the northern arid zone shared by Peru and Ecuador; the remaining species are Amazonian.

  16. Papel dos besouros (Insecta, Coleoptera na Entomologia Forense

    Directory of Open Access Journals (Sweden)

    Wellington Emanuel dos Santos

    2014-12-01

    Full Text Available Esse trabalho apresenta uma revisão do papel que os besouros (Insecta, Coleoptera desempenham na Entomologia Forense. Discussões sobre ocorrência em cadáveres humanos e carcaças animais, estimativas de Intervalo Pós-Morte (IPM, estudos realizados no Brasil e em outros países, principais famílias de importância forense e aspectos biológicos, ecológicos e biogeográficos das espécies são apresentadas.

  17. Checklist of beetles (Coleoptera of Canada and Alaska. Second edition

    Directory of Open Access Journals (Sweden)

    Yves Bousquet

    2013-12-01

    Full Text Available All 8237 species-group taxa of Coleoptera known to occur in Canada and Alaska are recorded by province/territory or state, along with their author(s and year of publication, in a classification framework. Only presence of taxa in each Canadian province or territory and Alaska is noted. Labrador is considered a distinct geographical entity. Adventive and Holarctic species-group taxa are indicated. References to pertinent identification keys are given under the corresponding supraspecific taxa in the data archive.

  18. Ground beetles (Coleoptera, Carabidae agrocenoses of spring and winter wheat

    Directory of Open Access Journals (Sweden)

    Luboš Purchart

    2005-01-01

    Full Text Available On two monitoring areas of the Central Institute for Supervising and Testing in Agriculture (ÚKZÚZ loaded with risk elements we carried out investigations of beetles of the family Carabidae (Coleoptera in agricultural stands of winter and spring wheat. The focus of the present study is on synecological characteristics and in some extent on the impact of agricultural practise on the population and seasonal dynamics of the most important representatives of ground beetles. This paper precedes the following article aimed to contents of heavy metals in ground beetles.

  19. Revision of the genus Endochilus Weise (Coleoptera: Coccinellidae: Chilocorini)

    Science.gov (United States)

    Łączyński, Piotr; Tomaszewska, Wioletta

    2014-01-01

    Abstract The members of the endemic African genus EndochilusWeise, 1898 (Coleoptera: Coccinellidae: Chilocorini) are redescribed, diagnosed, and illustrated. Lectotypes are designated for Endochilus compater Weise, Endochilus minor Weise, Endochilus plagiatus Sicard, Endochilus rubicundus Weise, and Endochilus styx Sicard. One new species is described: Endochilus abdominalissp. nov. Notes on the genus and nomenclatural history for each species are provided. A key for iden- tification of all species is presented. Adult characters concerning similarities of Endochilus to other genera of African Chilocorini are discussed. PMID:25373218

  20. Activation of dormant secondary metabolite production by introducing neomycin resistance into the deep-sea fungus, Aspergillus versicolor ZBY-3.

    Science.gov (United States)

    Dong, Yuan; Cui, Cheng-Bin; Li, Chang-Wei; Hua, Wei; Wu, Chang-Jing; Zhu, Tian-Jiao; Gu, Qian-Qun

    2014-07-29

    A new ultrasound-mediated approach has been developed to introduce neomycin-resistance to activate silent pathways for secondary metabolite production in a bio-inactive, deep-sea fungus, Aspergillus versicolor ZBY-3. Upon treatment of the ZBY-3 spores with a high concentration of neomycin by proper ultrasound irradiation, a total of 30 mutants were obtained by single colony isolation. The acquired resistance of the mutants to neomycin was confirmed by a resistance test. In contrast to the ZBY-3 strain, the EtOAc extracts of 22 of the 30 mutants inhibited the human cancer K562 cells, indicating that these mutants acquired a capability to produce antitumor metabolites. HPLC-photodiode array detector (PDAD)-UV and HPLC-electron spray ionization (ESI)-MS analyses of the EtOAc extracts of seven bioactive mutants and the ZBY-3 strain indicated that diverse secondary metabolites have been newly produced in the mutant extracts in contrast to the ZBY-3 extract. The followed isolation and characterization demonstrated that six metabolites, cyclo(D-Pro-D-Phe) (1), cyclo(D-Tyr-D-Pro) (2), phenethyl 5-oxo-L-prolinate (3), cyclo(L-Ile-L-Pro) (4), cyclo(L-Leu-L-Pro) (5) and 3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (6), were newly produced by the mutant u2n2h3-3 compared to the parent ZBY-3 strain. Compound 3 was a new compound; 2 was isolated from a natural source for the first time, and all of these compounds were also not yet found in the metabolites of other A. versicolor strains. Compounds 1-6 inhibited the K562 cells, with inhibition rates of 54.6% (1), 72.9% (2), 23.5% (3), 29.6% (4), 30.9% (5) and 51.1% (6) at 100 μg/mL, and inhibited also other human cancer HL-60, BGC-823 and HeLa cells, to some extent. The present study demonstrated the effectiveness of the ultrasound-mediated approach to activate silent metabolite production in fungi by introducing acquired resistance to aminoglycosides and its potential for discovering new compounds from silent fungal

  1. The prevalence of pityriasis versicolor in the dormitories of the Lorestan University of Medical Sciences by the three methods: skin scraping, use of scotch tape and wood’s lamp

    Directory of Open Access Journals (Sweden)

    sanaz Rostami

    2016-05-01

    Full Text Available Background : Pityriasis versicolor is a chronic, recurrent and often asymptomatic infection of stratum corneum, Which is caused by lipophilic yeasts of Malassezia. Human to human transmission through direct contact is possible. The present study aimed to assess the prevalence of pityriasis versicolor in the dormitories of the Lorestan University of Medical Sciences by the three methods: skin scraping, use of scotch tape and wood’s lamp was perfumed. Materials and Methods: In this descriptive cross-sectional study,115 students in the dormitories of the university of medical sciences were studied with the three methods:skin scraping, using  scotch tape and wood’s lamp. Results: Among 115 patients, 66.94% were female and 33.05% were male. The mean age was 21.38 years.25% of boys and 11.1% of girls were affected.The prevalence of  Pityriasis versicolor by  the three methods was determined 15.7%. In this study, the  most common site of infection was shoulder and neck. Conclusion: The recent study showed a high prevalence of Pityriasis versicolor in Lorestan University of  Medical Sciences students . The prevalence of pityriasis versicolor had a statistically significant difference between males and females, and was more common in females .  The pairwise comparison results obtained  from the three methods using the Kappa coefficient, scotch tape method  was the most consistent results than the other two methods.

  2. Rove beetles of medical importance in Brazil (Coleoptera, Staphylinidae, Paederinae

    Directory of Open Access Journals (Sweden)

    Juliana S. Vieira

    2014-09-01

    Full Text Available Rove beetles of medical importance in Brazil (Coleoptera, Staphylinidae, Paederinae. The rove beetles of the genus Paederus Fabricius, 1775 are the most important group within Coleoptera causing dermatitis around the world. The medical importance of Paederus depends on its toxic hemolymph released when these beetles are crushed on human skin. The effects are mainly dermatitis linearis and some sporadic cases of conjunctivitis. In Brazil seven species of Paederus are known to cause dermatitis: P. amazonicus Sharp, 1876, P. brasiliensis Erichson, 1840, P. columbinus Laporte, 1835, P. ferus Erichson, 1840, P. mutans Sharp, 1876, P. protensus Sharp, 1876 stat. rev., and Paederus rutilicornis Erichson, 1840. Paederus mutans and P. protensus are for the first time recorded as of medical importance, whereas the record of P. rutilicornis in Brazil is doubtful. All seven species are redescribed and a dichotomous key is provided. The geographic distributions of all species are documented. The results provided here include the most recent and relevant taxonomic revision of Paederus of the Neotropical region, the first identification key for Brazilian species and the increase of recorded species of medical importance in the world.

  3. Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tertiary Care Hospital, Punjab

    Directory of Open Access Journals (Sweden)

    M Kaur

    2013-01-01

    Full Text Available Purpose: Pityriasis versicolor (PV is a chronic superficial fungal disease caused by Malassezia species. Our aim was to identify Malassezia species from PV patients and healthy individuals in Punjab. Materials and Methods: Modified Dixon agar was used as isolation culture medium. Identification was based on morphological observation and biochemical evaluation. The biochemical evaluation consisted of culture onto Sabouraud dextrose agar, catalase reaction, Tween assimilation, Cremophor EL assimilation, splitting of esculin and growth at 38 0 C. Results: Out of 58 microscopically diagnosed cases of PV, growth was obtained from 54 (93.10% cases. The most frequently isolated species were M. globosa, M. sympodialis and M. furfur which made up 51.79%, 31.42% and 18.51% of the isolated etiological agents respectively. However, the major isolate from the back of healthy individuals was M. sympodialis (47.61%, followed by M.obtusa (19.04%, M. globosa (14.20%, M. furfur (9.52%, M. pachydermatis (4.76% and M. slooffiae (4.76%. Conclusions: M. globosa in its mycelial phase was the main etiological agent, but as normal flora from the back of healthy subjects, it was found in significantly less number (P = 0.01, suggesting that the higher pathogenicity of M. globosa in terms of enzymatic endowment, might be the cause of its predominance in PV lesions.

  4. DECOLORIZATION OF TEXTILE DYES BY NEWLY ISOLATED TRAMETES VERSICOLOR STRAIN Sevil PİLATİN, Buket KUNDUHOĞLU

    Directory of Open Access Journals (Sweden)

    Buket KUNDUHOĞLU

    2011-08-01

    Full Text Available In this century, the amount of industrial produces and their consumption has increased tremendously. Along with this increase, accumulation of industrial waste and its effect on nature has causedserious problems. Because of including various chemicals and especially dye, textile waste water is one of the most hazardous industrial wastes. Color is the most important pollutant in waste water and it should be decolorized. Decolorization is more important than degradation of organic substances from waste water. Even a small amount of dye found changes the color of rivers, lakes and other waterresources, and reduces the penetration of light and solubility of gases. White rot fungus are used as a biological system in degradation and decolorizing of textile dyes.In this study, parameters for decolorization of several textile dyes (Blue 49, Orange 12, Orange 13, Red 31, Black 5, RBBR by newly isolated Trametes versicolor M96was studied.It has been determined that pH, amount of inoculum, shaking speed (rpm, dye concentration and temperature are important factors in decolorization of the studied dyes. The maximum decolorization was found pH 4.5, amount of inoculums 50 ml, shaking speed 200 rpm, dye concentration 50 mg/l and heat 30 °C.

  5. Pomegranate peels: A novel ago-residue for tannate production by gamma irradiated aspergillus versicolor under solid state fermentation

    International Nuclear Information System (INIS)

    Khalaf, M.A.; Khalaf, S.A.

    2005-01-01

    Pomegranate peels were tested as a substrate for the production of tannate and gallic acid (GA) under solid state fermentation (SSF) using Aspergillus versicolor-g3B (highly potent gamma irradiated tannate producer isolate). The fungal strain was grown on the substrate without any pre-treatment. Different parameters for maximum production of tannate by this strain under SSF method were optimized. The maximum tannate yield (12.45μg-1ds) and GA (94%) were obtained when SSF was carried out at ph 5.0, 30 degree C, 60% initial substrate moisture, 20% inoculum size, 3% tannic acid and 1% ammonium nitrate as additional carbon and nitrogen sources, after 96 h of fermentation. The crude tannate from this isolate showed ph and temperature optima of 5.0 and 50 degree C, respectively. Also, the enzyme was stable in a broad ph range of 3-8 for 24 h and was also heat stable as it retained 65% activity at 70 degree C after 1 hour

  6. Aspectos clínicos de pacientes com pitiríase versicolor atendidos em um centro de referência em dermatologia tropical na cidade de Manaus (AM, Brasil Clinical aspects of patients with pityriasis versicolor seen at a referral center for tropical dermatology in Manaus, Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Patrícia Motta de Morais

    2010-12-01

    Full Text Available FUNDAMENTOS: A pitiríase versicolor (tinha versicolor é uma micose superficial crônica, causada por leveduras do gênero Malassezia spp. comensais das camadas queratinizadas da pele e que, sob determinadas condições ainda não esclarecidas, se torna patogênica, determinando as manifestações clínicas da doença. É uma dermatose recidivante e, mesmo após tratamento, pode deixar hipopigmentação persistente, causando problemas sociais aos indivíduos acometidos. OBJETIVO: Descrever as características clínicas e epidemiológicas de pacientes com diagnóstico de tinha versicolor atendidos em uma unidade de referência em Dermatologia (Fundação Alfredo da Matta. MÉTODOS: Estudo de série de casos em que foram detalhadas as manifestações cutâneas e as características epidemiológicas de pacientes atendidos na Fundação Alfredo da Matta com diagnóstico de tinha versicolor. RESULTADOS: Cento e dezesseis pacientes foram incluídos no estudo no período de janeiro a agosto de 2008. A maioria dos indivíduos é do sexo masculino, de cor parda, da faixa etária jovem e formada por estudantes, que apresentavam fatores predisponentes ao surgimento das manchas. Também a maioria apresentava lesões extensas e história passada da doença. CONCLUSÃO: O estudo mostrou alta proporção de indivíduos com quadros extensos e de longa duração da doença.BACKGROUND: Pityriasis versicolor (tinea versicolor is a chronic superficial mycosis caused by yeasts of the Malassezia spp. genus commensal of the keratinized layers of the skin. Under conditions not yet understood, it becomes pathogenic determining the clinical manifestations of the disease. It is a recurrent skin condition and persistent hypopigmentation may remain after treatment, causing social problems to those affected. OBJECTIVE: To describe the clinical and epidemiological features of patients diagnosed with tinea versicolor treated at a referral center for dermatology (Alfredo da

  7. Catalogue of Tenebrionidae (Coleoptera of North America

    Directory of Open Access Journals (Sweden)

    Yves Bousquet

    2018-01-01

    Full Text Available This catalogue includes all valid family-group (8 subfamilies, 52 tribes, 14 subtribes, genus-group (349 genera, 86 subgenera, and species-group names (2825 species, 215 subspecies of darkling beetles (Coleoptera: Tenebrionidae known to occur in North America1 and their available synonyms. Data on extant, subfossil and fossil taxa are given. For each name the author and year and page number of the description are provided, with additional information (e.g., type species for genus-group names, author of synonymies for invalid taxa depending on the taxon rank. Several new nomenclatural acts are included. One new genus, Lepidocnemeplatia Bousquet and Bouchard, is described. Spelaebiosis Bousquet and Bouchard [for Ardoinia Özdikmen, 2004], Blapstinus marcuzzii Aalbu [for Blapstinus kulzeri Marcuzzi, 1977], and Hymenorus campbelli Bouchard [for Hymenorus oculatus Doyen and Poinar, 1994] are proposed as new replacement names. Supporting evidence is provided for the conservation of usage of Tarpela micans (Fabricius, 1798 nomen protectum over Tarpela vittata (Olivier, 1793 nomen oblitum. The generic names Psilomera Motschulsky, 1870 [= Stenomorpha Solier, 1836], Steneleodes Blaisdell, 1909 [= Xysta Eschscholtz, 1829], Ooconibius Casey, 1895 and Euconibius Casey, 1895 [= Conibius LeConte, 1851] are new synonyms (valid names in square brackets. The following 127 new synonymies of species-group names, listed in their original combination, are proposed (valid names, in their current combination, placed in square brackets: Bothrasida mucorea Wilke, 1922 [= Pelecyphorus guanajuatensis (Champion, 1884]; Parasida zacualpanicola Wilke, 1922 [= Pelecyphorus asidoides Solier, 1836]; Stenosides kulzeri Pallister, 1954, Stenosides bisinuatus Pallister, 1954, and Parasida trisinuata Pallister, 1954 [= Pelecyphorus dispar (Champion, 1892]; Asida favosa Champion, 1884 and Asida similata Champion, 1884 [= Pelecyphorus fallax (Champion, 1884]; Ologlyptus bicarinatus

  8. Co-composting of spent coffee ground with olive mill wastewater sludge and poultry manure and effect of Trametes versicolor inoculation on the compost maturity.

    Science.gov (United States)

    Hachicha, Ridha; Rekik, Olfa; Hachicha, Salma; Ferchichi, Mounir; Woodward, Steve; Moncef, Nasri; Cegarra, Juan; Mechichi, Tahar

    2012-07-01

    The co-composting of spent coffee grounds, olive mill wastewater sludge and poultry manure was investigated on a semi-industrial scale. In order to reduce the toxicity of the phenolic fraction and to improve the degree of composting humification, composts were inoculated with the white-rot fungus Trametes versicolor in the early stages of the maturation phase. During composting, a range of physico-chemical parameters (temperature and both organic matter and C/N reduction), total organic carbon, total nitrogen, elemental composition, lignin degradation and spectroscopic characteristics of the humic acids (HAs) were determined; impacts of the composting process on germination index of Hordeum vulgare and Lactuca sativa were assessed. The coffee waste proved to be a highly compostable feedstock, resulting in mature final compost with a germination index of 120% in less than 5 months composting. In addition, inoculation with T. versicolor led to a greater degree of aromatization of HA than in the control pile. Moreover, in the inoculated mixture, lignin degradation was three times greater and HA increased by 30% (P<0.05), compared to the control pile. In the T. versicolor inoculated mixture, the averages of C and N were significantly enhanced in the HA molecules (P<0.05), by 26% and 22%, respectively. This improvement in the degree of humification was confirmed by the ratio of optical densities of HA solutions at 465 and 665 nm which was lower for HA from the treated mixture (4.5) than that from the control pile (5.4). Copyright © 2012 Elsevier Ltd. All rights reserved.

  9. Acoustic detection of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) and Oryctes elegans (Coleoptera: Scarabaeidae) in Phoenix dactylifera (Arecales: Arecacae) trees and offshoots in Saudi Arabian orchards

    Science.gov (United States)

    Rhynchophorus ferrugineus (Olivier) (Coleoptera: Dryophthoridae) larvae are cryptic, internal-tissue feeding pests of palm trees that are difficult to detect until after they have caused severe economic damage; consequently, infestations may remain undetected until they are widespread in an orchard....

  10. Single dose (400 mg) versus 7 day (200 mg) daily dose itraconazole in the treatment of tinea versicolor: a randomized clinical trial.

    Science.gov (United States)

    Wahab, M A; Ali, M E; Rahman, M H; Chowdhury, S A; Monamie, N S; Sultana, N; Khondoker, L

    2010-01-01

    Tinea (pityriasis) versicolor is a superficial fungal infection and one of the most commonly found pigmentary disorders of skin caused by the yeast Malassezia. Multiple topical as well as systemic therapies are available for treatment. Systemic therapies are used for extensive disease, frequent relapse or where topical agents have failed. The aim that translates the rationale of the study was to compare the efficacy, safety, tolerability and cost effectiveness of single dose 400mg versus 7 day 200 mg daily dose of itraconazole in the treatment of tinea versicolor. A clinical study was done to compare the efficacy of single dose (400 mg) of itraconazole and 7 day 200 mg daily dose of itraconazole in the treatment of extensive tinea versicolor. Total 60 patients (aged 18-50 years) were selected for the study during the period of June 2007 to May 2008 in the department of Dermatology of three different hospitals in Bangladesh. Cases having with extensive involvement, diagnosed clinically and confirmed by wood's lamp and KOH microscopy were taken. Patients were randomly allocated into equal groups. Group A was given single dose 400 mg itraconazole and Group B was given 7 day 200 mg daily itraconazole. Fifty three (88%) male and 7(12%) female were included in the study. The mean age of group A was 32.37+/-9 years and in group B 33.23+/-8 years. The mean duration of the disease in group A was 2.63+/-2 months and 2.76+/-2 months in group B. In group A clinical responders was found cure 22(73.33%) and improvement 5(16.33%) and in group B it was found cure 24(79.99%) and improvement 4(13.33%). The measure at the End point (EP1) equals to 90% response and in-group B it was found cure 24 (79.99%) and improvement 4(13.33%). (Here the End point EP2) equals to 93.33%. The EP clinical analysis however shows 91.66% response. Both single dose and 7 day daily dose of itraconazole can be effective in the treatment of tinea versicolor with extensive involvement but single dose appears

  11. Brachylophora, a new brachypterous genus of Rhopalophorini (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Robin O. S. Clarke

    2011-06-01

    Full Text Available Brachylophora, a new brachypterous genus of Rhopalophorini (Coleoptera, Cerambycidae. Brachylophora auricollis (Bruch, 1918 comb. nov. = Pasiphyle auricollis Bruch, 1918, originally described from Argentina (Salta, is redescribed and illustrated. Although with reduced elytra, the genus is transferred from Rhinotragini to Rhopalophorini based on the following characters: eyes well separated in both sexes, frons between eyes depressed and lacking frontal suture; pro-, meso-, and metasternum planar; mesothorax parallel-sided, not at all declivous before mesosternal process; metasternum large, together with mesosternum twice length of prosternum, metepisternum very wide, entire suture separating it from metasternum clearly visible when viewed from below; female ovipositor shortened with short cylindrical styles; and, more generally, structural features of hind legs, and surface ornamentation. Habitus similar to Coremia group. Bolivian specimens were netted as they visited flowers of Croton sp. (Euphorbiaceae.

  12. Diversidad de la familia Carabidae (Coleoptera en Chile Diversity of the family Carabidae (Coleoptera in Chile

    Directory of Open Access Journals (Sweden)

    SERGIO ROIG-JUÑENT

    2001-09-01

    Full Text Available Carabidae constituye dentro de los coléopteros chilenos, la cuarta familia en importancia por su cantidad de especies. El presente trabajo incluye una breve compilación acerca de la historia de la familia y de las primeras expediciones realizadas en Chile. También se realizan comparaciones de la diversidad de carábidos chilenos con respecto a otros países y el Neotrópico. Para Chile, se conocen 21 tribus, con 95 géneros y 365 especies, que representan el 38,8, 28,8 y 7,9 % de la fauna del Neotrópico, respectivamente. Chile posee un bajo número de tribus comparado con otros países, sin embargo, constituye un área importante por la presencia de seis tribus relictuales, principalmente pangeicas o gondwánicas. Chile posee 18 géneros endémicos (18,5 % de su fauna de Carabidae, 28 cuya distribución está restringida a Chile y Argentina y seis restringidos a Chile, Argentina y Uruguay. La cantidad de especies presentes en Chile es inferior a la que poseen otros países de América del Sur, pero la cantidad de especies endémicas es muy alta (204 y representa el 55,8 % de su fauna de carábidos. El alto grado de endemismo que posee Chile con respecto a otros países de América del Sur puede deberse a su condición de aislamiento, siendo las barreras más importantes la región desértica del norte y la cordillera de Los Andes. Este hecho también se vislumbra por la ausencia de importantes tribus neotropicales como Galeritini, Scaritini y Brachinini. También se incluyen en este trabajo claves para la identificación de todas las tribus y géneros presentes en Chile, como así también una breve descripción acerca de la diversidad y ambientes en los que se encuentra cada géneroThe family Carabidae is the fourth largest Coleoptera family in Chile. The present work includes a brief compilation on the taxonomic history of the family and the first expeditions to Chile. In addition, knowledge of carabid diversity in Chile is compared with

  13. Walking stability of Rhyzopertha dominica (Fabricius, 1792 (Coleoptera: Bostrichidae

    Directory of Open Access Journals (Sweden)

    E. M. Pires

    Full Text Available Abstract Results obtained in studies can contribute to the advancement of science and innovative methods and techniques for developing practical activities. Reporting conditions that may restrict the implementation of research is critical to ensure the optimal development of further technical studies. The objective of this study was to assess the walking stability of R. dominica on a flat and smooth surface. The study was based on the determination of mortality, morphology and walking stability of the insect outside the grain mass, on a flat and smooth surface. Mortality of adults of this Coleoptera in conditions with and without food was similar, which explains the difficulty that this insect had for accessing the food source on the flat and smooth surface. The measurements of body length (BOL, width (BOW and height (BOH of R. dominica were compared with those of Tribolium castaneum (Coleoptera: Tenebrionidae, which showed good ability to walk in these conditions. This study indicated that the former presents lower BOL and BOW, and greater BOH than the second, and all these variables showed differences when analyzed simultaneously by means of the construction of multivariate morphometric indices (Width × Height, Length × Height and Height × Length × Width. These morphometric variables, together with the definition of the geometry most similar to the body shape, resulted in determination of the center of gravity (CG and static rollover threshold (SRTgeom for both species. Rhyzopertha dominica and T. castaneum presented CGs considered high and low, respectively, and together with the values obtained for SRTgeom, may justify that R. dominica can be considered a less stable species during movement, and presents greater risk of rollover on flat and smooth surfaces.

  14. Coleoptera associated with macrophytes of the genus Salvinia in four oxbow lakes in two river basins in southeast Brazil

    Directory of Open Access Journals (Sweden)

    M. C. Paula-Bueno

    Full Text Available Abstract Macrophytes in oxbow lakes represent an important substrate for the Coleoptera. Two oxbow lakes the Rio Paranapanema were studied and the other two Rio Mogi-Guaçu, in the State de São Paulo, Brasil. In this study, there is greater similarity between the communities of Coleoptera of lakes greater connectivity with the main river channel or the difference in the species of Salvinia collected in the lakes studied interferes Coleoptera fauna that uses as substrate. A total of 9,222 specimens of Coleoptera were collected and identified in 10 families and 40 genera. The analysis MDS for abundance of Coleoptera showed the grouping of the oxbow lakes the Paranapanema River and a distancing the oxbow lakes the Mogi-Guaçu. The PERMANOVA test did not reveal any difference in the fauna between the wet and dry periods. It was concluded that the connectivity between river and lake is not decisive for the richness and abundance of aquatic fauna of Coleoptera. Therefore, the richness and abundance of aquatic Coleoptera associated vary with the species of Salvinia used as substrate.

  15. Coleoptera associated with macrophytes of the genus Salvinia in four oxbow lakes in two river basins in southeast Brazil.

    Science.gov (United States)

    Paula-Bueno, M C; Fonseca-Gessner, A A

    2015-11-01

    Macrophytes in oxbow lakes represent an important substrate for the Coleoptera. Two oxbow lakes the Rio Paranapanema were studied and the other two Rio Mogi-Guaçu, in the State de São Paulo, Brasil. In this study, there is greater similarity between the communities of Coleoptera of lakes greater connectivity with the main river channel or the difference in the species of Salvinia collected in the lakes studied interferes Coleoptera fauna that uses as substrate. A total of 9,222 specimens of Coleoptera were collected and identified in 10 families and 40 genera. The analysis MDS for abundance of Coleoptera showed the grouping of the oxbow lakes the Paranapanema River and a distancing the oxbow lakes the Mogi-Guaçu. The PERMANOVA test did not reveal any difference in the fauna between the wet and dry periods. It was concluded that the connectivity between river and lake is not decisive for the richness and abundance of aquatic fauna of Coleoptera. Therefore, the richness and abundance of aquatic Coleoptera associated vary with the species of Salvinia used as substrate.

  16. Diversity of forensic rove beetles (Coleoptera, Staphylinidae) associated with decaying pig carcass in a forest biotope.

    Science.gov (United States)

    Dekeirsschieter, Jessica; Frederick, Christine; Verheggen, Francois J; Drugmand, Didier; Haubruge, Eric

    2013-07-01

    Most forensic studies are focused on Diptera pattern colonization while neglecting Coleoptera succession. So far, little information is available on the postmortem colonization by beetles and the decomposition process they initiate under temperate biogeoclimatic countries. These beetles have, however, been referred to as being part of the entomofaunal colonization of a dead body. Forensic entomologists need increased databases detailing the distribution, ecology, and phenology of necrophagous insects, including staphylinids (Coleoptera, Staphylinidae). While pig carcasses are commonly used in forensic entomology studies to surrogate human decomposition and to investigate the entomofaunal succession, very few works have been conducted in Europe on large carcasses. Our work reports the monitoring of the presence of adult rove beetles (Coleoptera, Staphylinidae) on decaying pig carcasses in a forest biotope during four seasons (spring, summer, fall, and winter). A total of 23 genera comprising 60 species of rove beetles were collected from pig carcasses. © 2013 American Academy of Forensic Sciences.

  17. [Infestation of the human digestive system with beetle larvae (Coleoptera: Cantharidae): a case report].

    Science.gov (United States)

    Yılmaz, Hasan; Taş Cengiz, Zeynep; Dülger, Ahmet Cumhur; Ekici, Pınar

    2014-12-01

    This study was conducted to report the digestive system infestation caused by the larvae of Coleoptera in a female pediatric patient. She was admitted to our hospital with the complaints of emergence of insect larvae from her vomit and feces, abdominal pain, inguinal pain, lack of appetite, hair loss, excessive cleaning behavior, extreme irritability, and distractibility. The larvae observed typically had the morphology of the larvae of insects related to the Cantharidae family in the Coleoptera order. For treatment, a single dose of albendazole (400 mg) was used. Consequently, in the present case, it was seen that the larvae of Coleoptera incidentally taken orally could continue to live for a period in the digestive tract of people, without losing vitality, and the larvae caused a variety of symptoms due to both their toxic agents and the possible irritation they caused.

  18. UV laser radiation alters the embryonic protein profile of adrenal-kidney-gonadal complex and gonadal differentiation in the lizard, Calotes Versicolor.

    Science.gov (United States)

    Khodnapur, Bharati S; Inamdar, Laxmi S; Nindi, Robertraj S; Math, Shivkumar A; Mulimani, B G; Inamdar, Sanjeev R

    2015-02-01

    To examine the impact of ultraviolet (UV) laser radiation on the embryos of Calotes versicolor in terms of its effects on the protein profile of the adrenal-kidney-gonadal complex (AKG), sex determination and differentiation, embryonic development and hatching synchrony. The eggs of C. versicolor, during thermo-sensitive period (TSP), were exposed to third harmonic laser pulses at 355 nm from a Q-switched Nd:YAG laser for 180 sec. Subsequent to the exposure they were incubated at the male-producing temperature (MPT) of 25.5 ± 0.5°C. The AKG of hatchlings was subjected to protein analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and to histology. The UV laser radiation altered the expression of the protein banding pattern in the AKG complex of hatchlings and it also affected the gonadal sex differentiation. SDS-PAGE of AKG of one-day-old hatchlings revealed a total of nine protein bands in the control group whereas UV laser irradiated hatchlings expressed a total of seven protein bands only one of which had the same Rf as a control band. The UV laser treated hatchlings have an ovotestes kind of gonad exhibiting a tendency towards femaleness instead of the typical testes. It is inferred that 355 nm UV laser radiation during TSP induces changes in the expression of proteins as well as their secretions. UV laser radiation had an impact on the gonadal differentiation pathway but no morphological anomalies were noticed.

  19. Box-Behnken design for extraction optimization of crude polysaccharides from Tunisian Phormidium versicolor cyanobacteria (NCC 466): Partial characterization, in vitro antioxidant and antimicrobial activities.

    Science.gov (United States)

    Belhaj, Dalel; Frikha, Donyez; Athmouni, Khaled; Jerbi, Bouthaina; Ahmed, Mohammad Boshir; Bouallagui, Zouhaier; Kallel, Monem; Maalej, Sami; Zhou, John; Ayadi, Habib

    2017-12-01

    In this study, response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to optimize the aqueous extraction of crude polysaccharides from Tunisian cyanobacteria Phormidium versicolor (NCC 466). The optimal extraction conditions with an extraction yield of 21.56±0.92% were as follows: extraction temperature at 81.05°C, extraction time of 3.99h, and water to raw material ratio of 21.52mLg -1 . Crude Phormidium versicolor polysaccharides (CPv-PS) are found to be a hetero-sulfated-anionic polysaccharides that contained carbohydrate (79.37±1.58%), protein (0.45±0.11%), uronic acids (4.37±0.19%) and sulfate (6.83±0.28%). The carbohydrate fraction was composed of arabinose, xylose, ribose, rhamnose, N-acetyl glucosamine, galactose, glucose, mannose, glucuronic acid and saccharose with corresponding mole percentages of 2.41, 14.58, 2.18, 6.23, 7.04, 28.21, 26.04, 3.02, 0.86 and 5.07, respectively. Evaluation of the antioxidant activity in vitro suggested that CPv-PS strongly scavenged radicals, prevented bleaching of β-carotene and reduced activity. Furthermore, the CPv-PS exhibited effective antimicrobial properties. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

  20. Bio-edafology of the Coleoptera order, in three Colombia Natural Regions

    International Nuclear Information System (INIS)

    Camero R, Edgar; Chamorro B, Clara

    1997-01-01

    The characterization of Coleoptera families is showed to three different Colombian Natural Regions. This is given in relation with biological and environmental factors, different vegetation covers and soil uses. In addition to it, susceptible Coleoptera taxa are determined when natural conditions are disturbed. Methodically, a literature subject research was made, and Barber and Berlesse traps were used to organism extractions from superficial and under superficial soil. Horizons diversity, riches and constancy index were determined to each family. Results show different diversity, riches and constancy values to each family, as much in each natural region, such as to each soil use

  1. Characterization of purified and Xerogel immobilized Novel Lignin Peroxidase produced from Trametes versicolor IBL-04 using solid state medium of Corncobs

    Directory of Open Access Journals (Sweden)

    Asgher Muhammad

    2012-08-01

    Full Text Available Abstract Background Cost-effective production of industrially important enzymes is a key for their successful exploitation on industrial scale. Keeping in view the extensive industrial applications of lignin peroxidase (LiP, this study was performed to purify and characterize the LiP from an indigenous strain of Trametes versicolor IBL-04. Xerogel matrix enzyme immobilization technique was applied to improve the kinetic and thermo-stability characteristics of LiP to fulfil the requirements of the modern enzyme consumer sector of biotechnology. Results A novel LiP was isolated from an indigenous T. versicolor IBL-04 strain. T. versicolor IBL-04 was cultured in solid state fermentation (SSF medium of corn cobs and maximum LiP activity of 592 ± 6 U/mL was recorded after five days of incubation under optimum culture conditions. The crude LiP was 3.3-fold purified with specific activity of 553 U/mg after passing through the DEAE-cellulose and Sephadex-G-100 chromatography columns. The purified LiP exhibited a relatively low molecular weight (30 kDa homogenous single band on native and SDS-PAGE. The LiP was immobilized by entrapping in xerogel matrix of trimethoxysilane (TMOS and proplytetramethoxysilane (PTMS and maximum immobilization efficiency of 88.6% was achieved. The free and immobilized LiPs were characterized and the results showed that the free and immobilized LiPs had optimum pH 6 and 5 while optimum temperatures were 60°C and 80°C, respectively. Immobilization was found to enhance the activity and thermo-stability potential of LiP significantly and immobilized LiP remained stable over broad pH and temperature range as compare to free enzyme. Kinetic constants Km and Vmax were 70 and 56 μM and 588 and 417 U/mg for the free and immobilized LiPs, respectively. Activity of this novel extra thermo-stable LiP was stimulated to variable extents by Cu2+, Mn2+ and Fe2+ whereas, Cystein, EDTA and Ag+ showed inhibitory effects

  2. Responses by Dendroctonus frontalis and Dendroctonus mesoamericanus (Coleoptera: Curculionidae) to Ssemiochemical lures in Chiapas, Mexico: possible roles of pheromones during joint host attacks

    Science.gov (United States)

    Alicia Nino-Dominguez; Brian T. Sullivan; Jose H. Lopez-Urbina; Jorge E. Macias-Samano

    2016-01-01

    In southern Mexico and Central America, the southern pine beetle Dendroctonus frontalis Zimmermann (Coleoptera: Curculionidae: Scolytinae) commonly colonizes host trees simultaneously with Dendroctonus mesoamericanus Armend

  3. The use of Trametes versicolor laccase for the polymerization of aniline in the presence of vesicles as templates.

    Science.gov (United States)

    Junker, Katja; Kissner, Reinhard; Rakvin, Boris; Guo, Zengwei; Willeke, Martin; Busato, Stephan; Weber, Thomas; Walde, Peter

    2014-02-05

    The enzymatic polymerization of aniline to polyaniline (PANI) with Trametes versicolor laccase (TvL) as catalyst and dioxygen (O₂) as oxidant was investigated in an aqueous medium containing unilamellar vesicles with an average diameter of about 80 nm formed from AOT (=sodium bis(2-ethylhexyl) sulfosuccinate). Compared to the same reaction carried out with horseradish peroxidase isoenzyme C (HRPC) as catalyst and hydrogen peroxide (H₂O₂) as oxidant, notable differences were found in the kinetics of the reaction, as well as in the characteristics of the PANI obtained. Under comparable optimal conditions, which are pH 3.5 for TvL/O₂ and pH 4.3 for HRPC/H₂O₂, the reaction with TvL/O₂ was much slower than with HRPC/H₂O₂, i.e. ≈27 days vs. 1 day reaction time to reach equilibrium with >90% yield at 25 °C. Although in both cases, aniline monomer coupling occurred mainly via the carbon atom in para position of aniline, UV-vis-NIR absorption and EPR measurements indicate that the reaction with TvL/O₂ yielded mainly overoxidized products (with λ(max)=730 nm). These products had a lower amount of unpaired electrons if compared with the products obtained with HRPC/H₂O₂ (with λ(max)≈1000 nm, which is characteristic for the polaron state of PANI-ES, the emeraldine salt form of PANI). Similarly to previous findings with HRPC/H₂O₂, enzyme inactivation occurred during the polymerization also in the case of TvL/O₂. Since the aqueous PANI-vesicle suspensions obtained are of high colloidal stability, they can be used directly as ink in a conventional thermal inkjet printer for printing on paper or on surface treated polyimide films. Printed PANI-ES patterns on paper changed colour from green (emeraldine salt) to blue (emeraldine base) upon exposure to ammonia gas, demonstrating the expected ammonia sensing properties. Copyright © 2013 Elsevier Inc. All rights reserved.

  4. Role of emerald ash borer (Coleoptera: Buprestidae) larval vibrations in host-quality assessments by Tetrastichus planipennisi (Hymenoptera: Eulophidae)

    Science.gov (United States)

    Michael D. Ulyshen; Richard W. Mankin; Yigen Chen; Jian J. Duan; Therese M. Poland; Leah S. Bauer

    2011-01-01

    The biological control agent Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) is a gregarious larval endoparasitoid of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive cambium-feeding species responsible for recent, widespread mortality of ash (Fraxinus spp.) in...

  5. Pityriasis versicolor: clinical-epidemiological characterization of patients in the urban area of Buerarema-BA , Brazil Pitiríase versicolor: caracterização clínico-epidemiológica em pacientes da área urbana de Buerarema-BA, Brasil

    Directory of Open Access Journals (Sweden)

    Juliano Oliveira Santana

    2013-04-01

    Full Text Available BACKGROUND: Pityriasis versicolor (PV is a cutaneous pigmentation disorder caused by a lipophilic yeast of the genus Malassezia sp. It is a superficial mycosis characterized by well-defined, slightly scaly skin lesions of variable color. In Brazil, the number of reported cases is small, and there are few epidemiological studies. OBJECTIVES: to assess incidence, characteristics of the lesions, effectiveness of the Zileri's Sign procedure, and the epidemiological profile of PV in the urban area of Buerarema - Bahia. METHODS: Biological samples were collected on pre-established days at Basic Health Care Units from July to September 2010. Sample collection was followed by laboratory diagnosis using Porto's Method. RESULTS: Of the 158 patients with suspected PV participating in the study, 105 (66.5% were positive; 72 (68.6% were female and 33 (31.4% were male. Sex and location of lesions showed statistically significant differences (p FUNDAMENTOS: A pitiríase versicolor é um distúrbio de pigmentação cutânea causada pela levedura lipofílica do gênero Malassezia sp. É uma micose superficial caracterizada por produzir lesões delimitadas, com descamação fina e de cor variável. No Brasil, o relato do número de casos e de estudos é restrito. OBJETIVOS: Analisar a incidência, as características das lesões, a eficácia do Sinal de Zileri e o perfil epidemiológico da pitiríase versicolor na área urbana do município de Buerarema-BA. MÉTODOS: Foram coletadas amostras biológicas no período de julho a setembro de 2010, em dias pré-establecidos, nas Unidades Básicas de Saúde. O Método de Porto foi utilizado como diagnóstico laboratorial. RESULTADOS: Dos 158 pacientes cadastrados com suspeita de pitiríase versicolor, 105 (66,5% mostraram-se positivos, sendo 72 (68,6% do sexo feminino e 33 (31,4% do sexo oposto. Ocorreu diferença estatisticamente significante entre sexo e localização das lesões (p<0,05. A região com maior

  6. Vertical Distribution and Daily Flight Periodicity of Ambrosia Beetles (Coleoptera: Curculionidae) in Florida Avocado Orchards Affected by Laurel Wilt.

    Science.gov (United States)

    Menocal, Octavio; Kendra, Paul E; Montgomery, Wayne S; Crane, Jonathan H; Carrillo, Daniel

    2018-03-08

    Ambrosia beetles have emerged as significant pests of avocado ((Persea americana Mill. [Laurales: Lauraceae])) due to their association with pathogenic fungal symbionts, most notably Raffaelea lauricola T.C. Harr., Fraedrich & Aghayeva (Ophiostomatales: Ophiostomataceae), the causal agent of the laurel wilt (LW) disease. We evaluated the interaction of ambrosia beetles with host avocado trees by documenting their flight height and daily flight periodicity in Florida orchards with LW. Flight height was assessed passively in three avocado orchards by using ladder-like arrays of unbaited sticky traps arranged at three levels (low: 0-2 m; middle: 2-4 m; high: 4-6 m). In total, 1,306 individuals of 12 Scolytinae species were intercepted, but six accounted for ~95% of the captures: Xyleborus volvulus (Fabricius) (Coleoptera: Curculionidae), Xyleborinus saxesenii Ratzeburg (Coleoptera: Curculionidae), Euplatypus parallelus (Fabricius) (Coleoptera: Curculionidae), Xyleborus bispinatus Eichhoff (Coleoptera: Curculionidae), Xyleborus affinis Eichhoff (Coleoptera: Curculionidae), and Hypothenemus sp. (Coleoptera: Curculionidae). The primary vector of R. lauricola, Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae), was not detected. Females of X. volvulus showed a preference for flight at low levels and X. bispinatus for the low and middle levels; however, captures of all other species were comparable at all heights. At a fourth orchard, a baiting method was used to document flight periodicity. Females of X. saxesenii and Hypothenemus sp. were observed in flight 2-2.5 h prior to sunset; X. bispinatus, X. volvulus, and X. affinis initiated flight at ~1 h before sunset and Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae) at 30 min prior to sunset. Results suggest that ambrosia beetles in South Florida fly near sunset (when light intensity and wind speed decrease) at much greater heights than previously assumed and have species-specific patterns in host

  7. Bioacoustics of Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) on Phaseolus vulgaris (Fabaceae)

    Science.gov (United States)

    Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae), is an economically important pest of common bean Phaseolus vulgaris L. (Fabaceae) in the tropics and subtropics. It is difficult to detect the presence of A. obtectus because the larvae are cryptic and spend most of their developmental time...

  8. Hydraena Kugelann, 1794 (Coleoptera, Hydraenidae from the Seychelles, Indian Ocean, with description of a new species

    Directory of Open Access Journals (Sweden)

    Manfred A. Jäch

    2016-10-01

    Full Text Available Hydraena matyoti sp. n. (Coleoptera, Hydraenidae is described from the Seychelles, Indian Ocean. Hydraena mahensis Scott, 1913 is redescribed. The latter is here recorded from La Digue for the first time. A key to the species of the genus Hydraena Kugelann, 1794 of the Seychelles is presented.

  9. Hydraena Kugelann, 1794 (Coleoptera, Hydraenidae) from the Seychelles, Indian Ocean, with description of a new species.

    Science.gov (United States)

    Jäch, Manfred A; Delgado, Juan A

    2016-01-01

    Hydraena matyoti sp. n. (Coleoptera, Hydraenidae) is described from the Seychelles, Indian Ocean. Hydraena mahensis Scott, 1913 is redescribed. The latter is here recorded from La Digue for the first time. A key to the species of the genus Hydraena Kugelann, 1794 of the Seychelles is presented.

  10. Review of the genus Ceresium Newman, 1842 (Coleoptera: Cerambycidae) in Fiji

    Science.gov (United States)

    A taxonomic review of the genus Ceresium (Coleoptera: Cerambycidae) found within the Fiji Islands is presented. A total of 17 species is treated. Full morphological descriptions and comparative images of each species are included, along with a dichotomous key for their identification....

  11. Review of the genus Ceresium Newman, 1842 (Coleoptera, Cerambycidae) in Fiji

    Science.gov (United States)

    Waqa-Sakiti, Hilda; Winder, Linton; Lingafelter, Steven W.

    2015-01-01

    Abstract A taxonomic review of the genus Ceresium (Coleoptera: Cerambycidae) found within the Fiji Islands is presented. A total of 17 species is treated. Full morphological descriptions and comparative images of each species are included, along with a dichotomous key for their identification. PMID:26692805

  12. Use of nutrient self selection as a diet refining tool in Tenebrio molitor (Coleoptera: Tenebrionidae)

    Science.gov (United States)

    A new method to refine existing dietary supplements for improving production of the yellow mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae), was tested. Self selected ratios of 6 dietary ingredients by T. molitor larvae were used to produce a dietary supplement. This supplement was compared...

  13. Impact of Adult Weight, Density, and Age on Reproduction of Tenebrio molitor (Coleoptera: Tenebrionidae)

    Science.gov (United States)

    The impact of adult weight, age, and density on reproduction of Tenebrio molitor L. (Coleoptera: Tenebrionidae) was studied. The impact of adult weight on reproduction was determined in two ways: 1) counting the daily progeny of individual adult pairs of known weight and analyzing the data with line...

  14. Temperature influences on diapause induction and survival in the boll weevil (Coleoptera: Curculionidae)

    Science.gov (United States)

    The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), has been the most important pest of cotton (Gossypium spp.) wherever it occurs. Although eradication programs in the U.S. have reduced the range of this pest, the weevil remains an intractable problem in subtropical Tex...

  15. Development of an improved attractive lure for the pine shoot beetle, Tomicus piniperda (Coleoptera: Scolytidae)

    Science.gov (United States)

    Therese M. Poland; Peter de Groot; Stephen Burke; David Wakarchuk; Robert A. Haack; Reginald Nott; Taylor Scarr

    2003-01-01

    1) The pine shoot beetle, Tomicus piniperda (L.) (Coleoptera: Scolytidae), is an exotic pest of pine, Pinus spp., and was first discovered in North America in 1992. 2) Although primary attraction to host volatiles has been clearly demonstrated for T. piniperda, the existence and role of secondary attraction to...

  16. Influence of temperature on spring flight initiation for southwestern ponderosa pine bark beetles (Coleoptera: Curculionidae, Scolytinae)

    Science.gov (United States)

    M. L. Gaylord; K. K. Williams; R. W. Hofstetter; J. D. McMillin; T. E. Degomez; M. R. Wagner

    2008-01-01

    Determination of temperature requirements for many economically important insects is a cornerstone of pest management. For bark beetles (Coleoptera: Curculionidae, Scolytinae), this information can facilitate timing of management strategies. Our goals were to determine temperature predictors for flight initiation of three species of Ips bark beetles...

  17. Tanyproctus (Tanyproctus) arher (Coleoptera: Scarabaeidae: Melolonthinae: Tanyproctini), a new species from the Socotra Island, Yemen

    Czech Academy of Sciences Publication Activity Database

    Bezděk, Aleš; Sehnal, R.; Král, D.

    2013-01-01

    Roč. 3737, č. 2 (2013), s. 191-196 ISSN 1175-5326 Institutional support: RVO:60077344 Keywords : Coleoptera * Scarabaeoidea * Scarabaeidae Subject RIV: EG - Zoology Impact factor: 1.060, year: 2013 http://www.mapress.com/zootaxa/2013/f/zt03737p196.pdf

  18. Stomanomala subcostata (Coleoptera: Scarabaeidae), the first record of ruteline chafer from Socotra Archipelago, Yemen

    Czech Academy of Sciences Publication Activity Database

    Bezděk, Aleš; Král, D.; Limbourg, P.

    2017-01-01

    Roč. 57, č. 3 (2017), s. 87-91 ISSN 0374-1036 Institutional support: RVO:60077344 Keywords : Coleoptera * Scarabaeidae * Rutelinae Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.632, year: 2016 https://www.biotaxa.org/AEMNP/article/view/35053

  19. Microbial control of Agrilus planipennis (Coleoptera: Buprestidae) with Beauveria bassiana strain GHA: field applications

    Science.gov (United States)

    Houping Liu; Leah S. Bauer

    2008-01-01

    The effects of Beauveria bassiana strain GHA, applied as BotaniGard ES, on newly colonised and well-established populations of emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae) were evaluated in the field using foliar and trunk sprays in Michigan in 2004-2005. Results from field trials at a newly colonised white ash...

  20. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae from Korea

    Directory of Open Access Journals (Sweden)

    Ik Je Choi

    2016-03-01

    Full Text Available A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  1. Molecular Diagnostic for Boll Weevil (Coleoptera: Curculionidae) Based on Amplification of Three Species-specific Microsatellites

    Science.gov (United States)

    The boll weevil, Anthonomus grandis Boheman (Coleoptera: Curculionidae), is a serious pest of cultivated cotton in the Americas, and reinfestation of zones from which they have been eradicated is of perpetual concern. Extensive arrays of pheromone traps monitor for reintroductions, but occasionally...

  2. Flight propensty of Anoplophora glabripennis, an Asian longhorned beetle (Coleoptera: Cerambycidae)

    Science.gov (United States)

    J. A. Francese; B. Wang; D. R. Lance; Z. Xu; S. Zong; Y. Luo; A. J. Sawyer; V. C. Mastro

    2003-01-01

    Anoplophora glabripennis (Coleoptera: Cerambycidae) (Motschulsky), is a recently introduced pest of hardwoods. Research to study its flight behavior was conducted in the field in Ningxia Autonomous Region, Peoples' Republic of China. To study the flight propensity of A. glabripennis, adult beetles were observed in population...

  3. Characterization of an Aggregation Pheromone in Hylesinus pruinosus (Coleoptera: Curculionidae: Scolytinae)

    Science.gov (United States)

    William Shepherd; Brian Sullivan; Bradley Hoosier; JoAnne Barrett; Tessa Bauman

    2010-01-01

    We conducted laboratory and field bioassays to characterize the pheromone system of an ash bark beetle, Hylesinus pruinosus Eichhoff (Coleoptera: Curculionidae: Scolytinae). Solitary females in newly initiated galleries in ash logs produced (+)-exo-brevicomin, whereas male beetles paired with females produced (+)-endo-brevicomin, lesser quantities of...

  4. First contact pheromone identified for a longhorned beetle (Coleoptera: Cerambycidae) in the subfamily Prioninae

    Science.gov (United States)

    Annie E. Spikes; Matthew A. Paschen; Jocelyn G. Miller; Jardel A. Moreira; Paul B. Hamel; Nathan M. Schiff; Matthew D. Ginzel

    2010-01-01

    Little is known of the reproductive behavior of longhorned beetles (Coleoptera: Cerambycidae) in the subfamily Prioninae. Mallodon dasystomus (Say), the hardwood stump borer, is a widely distributed prionine that is native to the southern U.S. Here, we explored the chemically-mediated mating behavior of M dasystomus, and tested the hypothesis that males recognize...

  5. Variation in enantiospecific attraction of Ips avulsus (Coleoptera: Curculionidae) to the pheromone ipsdienol in Georgia.

    Science.gov (United States)

    Daniel Miller; Jeremy Allison

    2011-01-01

    In 2006, we tested the responses of the small southern pine engraver, Ips avulsus (Eichhoff) (Coleoptera: Curculionidae), to multiple-funnel traps baited with (+)-, (-)-, and (+/-)- ipsdienol. Three experiments were conducted in Georgia with all traps co-baited with one of the following lure combinations, respectively: experiment 1, ipsenol; experiment 2, lanierone and...

  6. Description of a new species of Bothynus Hope from Argentina and Bolivia (Coleoptera: Scarabaeidae: Dynastinae).

    Science.gov (United States)

    Hardy, Martin

    2017-12-04

    A new species of Bothynus Hope, 1837 (Coleoptera: Scarabaeidae: Dynastinae: Pentodontini) is described from the eastern Andes in South America. The new species is unique in the genus for having the elytra and part of the pronotum covered with small setae.

  7. Host range expansion and increased damage potential of Euwallacea nr. fornicatus (Coleoptera: Curculionidae) in Florida

    Science.gov (United States)

    Ambrosia beetles in the Euwallacea nr. fornicatus complex (Coleoptera: Curculionidae) vector Fusarium spp. fungi pathogenic to susceptible hosts, including avocado. The Florida avocado production area in Miami-Dade County was surveyed for E. nr. fornicatus upon observations of initial damage in 2016...

  8. Behavioral assays for evaluating host preferences of Euwallacea nr. fornicatus (Coleoptera: Curculionidae: Scolytinae).

    Science.gov (United States)

    In 2010, the exotic ambrosia beetle, Euwallacea nr. fornicatus Eichhoff (Coleoptera: Curculionidae: Scolytinae) was first discovered in Florida avocado groves. Introduction of its symbiotic Fusarium spp. fungi into galleries in the xylem tissue results in Fusarium-dieback disease. Unlike most ambros...

  9. Dispersal of Agrilus planipennis (Coleoptera: Buprestidae) from discrete epicenters in two outlier sites

    Science.gov (United States)

    N.W. Siegert; D.G. McCullough; D.W. Williams; I. Fraser; T.M. Poland; S.J. Pierce

    2010-01-01

    Emerald ash borer, Agrilus planipennis (Fairmaire) (Coleoptera: Buprestidae), a phloem- feeding beetle native to Asia, has become one of the most destructive forest pests in North America. Since it was Þrst identified in 2002 in southeast Michigan and Windsor, Ontario, dozens of isolated A. planipennis populations have been...

  10. A comparison of trap type and height for capturing cerambycid beetles (Coleoptera)

    Science.gov (United States)

    Elizabeth E. Graham; Therese M. Poland; Deborah G. McCullough; Jocelyn G. Millar

    2012-01-01

    Wood-boring beetles in the family Cerambycidae (Coleoptera) play important roles in many forest ecosystems. However, increasing numbers of invasive cerambycid species are transported to new countries by global commerce and threaten forest health in the United States and worldwide. Our goal was to identify effective detection tools for a broad array of cerambycid...

  11. De brede geelgerande waterroofkever Dytiscus latissimus na 38 jaar weer in Nederland opgedoken (Coleoptera: Dytiscidae).

    NARCIS (Netherlands)

    Dijk, van G.

    2006-01-01

    Dytiscus latissimus after 38 years rediscovered in the Netherlands in 2005 (Coleoptera: Dytiscidae) In 2005 two males of the water beetle Dytiscus latissimus were caught near Uffelte (province of Drenthe). The species is endangered throughout its range and was thought to be extinct in the

  12. Distribution and habitat of Graphoderus bilineatus in the Netherlands (Coleoptera: Dytiscidae).

    NARCIS (Netherlands)

    Cuppen, J.G.M.; Koese, B.; Sierdsema, H.

    2006-01-01

    Verspreiding en biotopen van Graphoderus bilineatus (Coleoptera: Dytiscidae) De verspreiding en biotopen van de gestreepte waterroofkever Graphoderus bilineatus zijn in 2004 en 2005 onderzocht in opdracht van de provincie Zuid-Holland en het Ministerie van lnv. Deze waterroofkever is wettelijk

  13. Canuschiza of Socotra Island (Coleoptera: Scarabaeidae: Melolonthinae). Part 2. Canuschiza minuta species group

    Czech Academy of Sciences Publication Activity Database

    Sehnal, R.; Král, D.; Bezděk, Aleš

    2017-01-01

    Roč. 57, č. 3 (2017), s. 77-86 ISSN 0374-1036 Institutional support: RVO:60077344 Keywords : Coleoptera * Scarabaeoidea * Scarabaeidae Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.632, year: 2016 https://www.biotaxa.org/AEMNP/article/view/35052

  14. Ground beetle (Coleoptera: Carabidae) assemblages in the Conservation Reserve Program crop rotation systems in Interior Alaska

    Science.gov (United States)

    Adult ground beetles (Coleoptera: Carabidae) abundance and diversity were documented on Conservation Research Program (CRP) agricultural lands in Delta Junction, Alaska (64ºN, 145º W). Twenty species were documented based on a total sample of 6,116 specimens collected during 2006 and 2007. Two speci...

  15. Upper lethal temperature limits of the common furniture beetle Anobium punctatum (Coleoptera: Anobiidae)

    DEFF Research Database (Denmark)

    Hansen, Lise Stengård; Jensen, Karl-Martin Vagn

    1996-01-01

    The susceptibility of the egg, larval and adult stages of Anobium punctatum De Geer (Coleoptera: Anobiidae) to heat (46-54°C, 25-30% RH) was investigated. The larval stage was found to be most tolerant to heat. Very short exposure (5 min) of the larvae to temperatures of 52°C and above led to 100...

  16. A contribution to the rove beetles (Coleoptera: Staphylinidae, Paederinae in north of Iran

    Directory of Open Access Journals (Sweden)

    Masoud Mohammadi Dehcheshmeh

    2016-12-01

    Full Text Available In this paper, 19 species of rove beetles (Coleoptera: Staphylinidae, belonging to the subfamily Paederinae Fleming 1821, were collected from Mazandaran province, north of Iran, during 2015-2016. Two species, Rugilus angustatus Geoffroy 1758 and Astenus lyonessius (joy 1908 are reported for the first time from Iran.

  17. Repeated losses of TTAGG telomere repeats in evolution of beetles (Coleoptera)

    Czech Academy of Sciences Publication Activity Database

    Frydrychová, Radmila; Marec, František

    2002-01-01

    Roč. 115, - (2002), s. 179-187 ISSN 0016-6707 R&D Projects: GA ČR GA206/00/0750; GA AV ČR KSK5052113 Institutional research plan: CEZ:AV0Z5007907 Keywords : chromosomes * Coleoptera * phylogeny Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.063, year: 2002

  18. Patterns of tree species usage by long-horned beetles (Coleoptera: Cerambycidae) in Fiji

    Czech Academy of Sciences Publication Activity Database

    Waqa-Sakiti, H.; Stewart, A.; Čížek, Lukáš; Hodge, S.

    2014-01-01

    Roč. 68, č. 1 (2014), s. 57-64 ISSN 0030-8870 R&D Projects: GA ČR GAP504/12/1952 Institutional support: RVO:60077344 Keywords : Coleoptera Subject RIV: EH - Ecology, Behaviour Impact factor: 0.924, year: 2014 http://www.bioone.org/doi/pdf/10.2984/68.1.5

  19. Four new species of Phyllophaga Harris (Coleoptera: Scarabaeidae: Melolonthinae) from western Cuba.

    Science.gov (United States)

    Serrano, Annery; Morón, Miguel Ángel

    2017-12-07

    Four new species in the genus Phyllophaga Harris 1827, subgenus Cnemarachis Saylor 1942 (Coleoptera: Scarabaeidae: Melolonthinae) from Cuba are described: Phyllophaga barroi new species, Phyllophaga guanahacabibensis new species, Phyllophaga pauli new species, and Phyllophaga sabanalamarensis new species. Each species is illustrated using photographs of the habitus, diagnostic features, and male aedeagi. A map is provided showing the geographical distributions of the new species.

  20. Trapping Phyllophaga spp. (Coleoptera: Scarabaeidae: Melolonthinae) in the United States and Canada using sex attractants

    Science.gov (United States)

    Paul S. Robbins; Steven R. Alm; Charles D. Armstrong; Anne L. Averill; Thomas C. Baker; Robert J. Bauernfiend; Frederick P. Baxendale; S. Kris Braman; Rick L. Brandenburg; Daniel B. Cash; Gary J. Couch; Richard S. Cowles; Robert L. Crocker; Zandra D. DeLamar; Timothy G. Dittl; Sheila M. Fitzpatrick; Kathy L. Flanders; Tom Forgatsch; Timothy J. Gibb; Bruce D. Gill; Daniel O. Gilrein; Clyde S. Gorsuch; Abner M. Hammond; Patricia D. Hastings; David W. Held; Paul R. Heller; Rose T. Hiskes; James L. Holliman; William G. Hudson; Michael G. Klein; Vera L. Krischik; David J. Lee; Charles E. Linn; Nancy J. Luce; Kenna E. MacKenzie; Catherine M. Mannion; Sridhar Polavarapu; Daniel A. Potter; Wendell L. Roelofs; Brian M. Rovals; Glenn A. Salsbury; Nathan M. Schiff; David J. Shetlar; Margaret Skinner; Beverly L. Sparks; Jessica A. Sutschek; Timothy P. Sutschek; Stanley R. Swier; Martha M. Sylvia; Niel J. Vickers; Patricia J. Vittum; Richard Weidman; Donald C. Weber; R. Chris Williamson; Michael G. Villani

    2006-01-01

    The sex pheromone of the scarab beetle, Phyllophaga anxia, is a blend of the methyl esters of two amino acids, L-valine and L-isoleucine. A field trapping study was conducted, deploying different blends of the two compounds at 59 locations in the United States and Canada. More than 57,000 males of 61 Phyllophaga species (Coleoptera...

  1. Susceptibility of Agrilus planipennis (Coleoptera: Buprestidae) to Beauveria bassiana and Metarhizium anisopliae

    Science.gov (United States)

    Houping Lui; Leah S. Bauer

    2006-01-01

    The susceptibility of Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) to selected strains of the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin was evaluated through bioassays with direct immersion or foliar exposure under laboratory conditions. Results showed that A. planipennis adults were...

  2. Entomopathogens in conjunction with imidacloprid could be used to manage wireworms (Coleoptera: Elateridae) on spring wheat

    Science.gov (United States)

    The soil-dwelling larvae of click beetles (wireworms) (Coleoptera: Elateridae) are serious pests of several agricultural crops worldwide. Hypnoidus bicolor and Limonius californicus are two major wireworm species damaging to spring wheat, particularly in the Golden Triangle, an important cereal-grow...

  3. Plant compounds insecticide activity against Coleoptera pests of stored products

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Marcio Dionizio; Picanco, Marcelo Coutinho; Guedes, Raul Narciso Carvalho; Campos, Mateus Ribeiro de; Silva, Gerson Adriano; Martins, Julio Claudio [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Biologia Animal]. E-mail: marcio.dionizio@gmail.com; picanco@ufv.br; guedes@ufv.br; mateusc3@yahoo.com.br; agronomiasilva@yahoo.com.br

    2007-07-15

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on R. dominica was evaluated. Among them, only hexane extract of A. conyzoides showed insecticide activity; the hexane extract of this species was successively fractionated by silica gel column chromatography, for isolation and purification of the active compounds. Compounds 5,6,7,8,3',4',5'-heptamethoxyflavone; 5,6,7,8,3'-pentamethoxy-4',5'-methilenedioxyflavone and coumarin were identified. However, only coumarin showed insecticide activity against three insect pests (LD{sub 50} from 2.72 to 39.71 mg g{sup -1} a.i.). The increasing order of insects susceptibility to coumarin was R. dominica, S. zeamais and O. surinamensis. (author)

  4. DNA barcoding of Japanese click beetles (Coleoptera, Elateridae).

    Science.gov (United States)

    Oba, Yuichi; Ôhira, Hitoo; Murase, Yukio; Moriyama, Akihiko; Kumazawa, Yoshinori

    2015-01-01

    Click beetles (Coleoptera: Elateridae) represent one of the largest groups of beetle insects. Some click beetles in larval form, known as wireworms, are destructive agricultural pests. Morphological identification of click beetles is generally difficult and requires taxonomic expertise. This study reports on the DNA barcoding of Japanese click beetles to enable their rapid and accurate identification. We collected and assembled 762 cytochrome oxidase subunit I barcode sequences from 275 species, which cover approximately 75% of the common species found on the Japanese main island, Honshu. This barcode library also contains 20 out of the 21 potential pest species recorded in Japan. Our analysis shows that most morphologically identified species form distinct phylogenetic clusters separated from each other by large molecular distances. This supports the general usefulness of the DNA barcoding approach for quick and reliable identification of Japanese elaterid species for environmental impact assessment, agricultural pest control, and biodiversity analysis. On the other hand, the taxonomic boundary in dozens of species did not agree with the boundary of barcode index numbers (a criterion for sequence-based species delimitation). These findings urge taxonomic reinvestigation of these mismatched taxa.

  5. DNA barcoding of Japanese click beetles (Coleoptera, Elateridae.

    Directory of Open Access Journals (Sweden)

    Yuichi Oba

    Full Text Available Click beetles (Coleoptera: Elateridae represent one of the largest groups of beetle insects. Some click beetles in larval form, known as wireworms, are destructive agricultural pests. Morphological identification of click beetles is generally difficult and requires taxonomic expertise. This study reports on the DNA barcoding of Japanese click beetles to enable their rapid and accurate identification. We collected and assembled 762 cytochrome oxidase subunit I barcode sequences from 275 species, which cover approximately 75% of the common species found on the Japanese main island, Honshu. This barcode library also contains 20 out of the 21 potential pest species recorded in Japan. Our analysis shows that most morphologically identified species form distinct phylogenetic clusters separated from each other by large molecular distances. This supports the general usefulness of the DNA barcoding approach for quick and reliable identification of Japanese elaterid species for environmental impact assessment, agricultural pest control, and biodiversity analysis. On the other hand, the taxonomic boundary in dozens of species did not agree with the boundary of barcode index numbers (a criterion for sequence-based species delimitation. These findings urge taxonomic reinvestigation of these mismatched taxa.

  6. Economic analysis of emerald ash borer (Coleoptera: Buprestidae) management options.

    Science.gov (United States)

    Vannatta, A R; Hauer, R H; Schuettpelz, N M

    2012-02-01

    Emerald ash borer, Agrilus planipennis (Fairmaire) (Coleoptera: Buprestidae), plays a significant role in the health and extent of management of native North American ash species in urban forests. An economic analysis of management options was performed to aid decision makers in preparing for likely future infestations. Separate ash tree population valuations were derived from the i-Tree Streets program and the Council of Tree and Landscape Appraisers (CTLA) methodology. A relative economic analysis was used to compare a control option (do-nothing approach, only removing ash trees as they die) to three distinct management options: 1) preemptive removal of all ash trees over a 5 yr period, 2) preemptive removal of all ash trees and replacement with comparable nonash trees, or 3) treating the entire population of ash trees with insecticides to minimize mortality. For each valuation and management option, an annual analysis was performed for both the remaining ash tree population and those lost to emerald ash borer. Retention of ash trees using insecticide treatments typically retained greater urban forest value, followed by doing nothing (control), which was better than preemptive removal and replacement. Preemptive removal without tree replacement, which was the least expensive management option, also provided the lowest net urban forest value over the 20-yr simulation. A "no emerald ash borer" scenario was modeled to further serve as a benchmark for each management option and provide a level of economic justification for regulatory programs aimed at slowing the movement of emerald ash borer.

  7. Plant compounds insecticide activity against Coleoptera pests of stored products

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Marcio Dionizio; Picanco, Marcelo Coutinho; Guedes, Raul Narciso Carvalho; Campos, Mateus Ribeiro de; Silva, Gerson Adriano; Martins, Julio Claudio [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Biologia Animal; br, picanco@ufv; br, guedes@ufv; br, mateusc3@yahoo com; br, agronomiasilva@yahoo com

    2007-07-15

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on R. dominica was evaluated. Among them, only hexane extract of A. conyzoides showed insecticide activity; the hexane extract of this species was successively fractionated by silica gel column chromatography, for isolation and purification of the active compounds. Compounds 5,6,7,8,3',4',5'-heptamethoxyflavone; 5,6,7,8,3'-pentamethoxy-4',5'-methilenedioxyflavone and coumarin were identified. However, only coumarin showed insecticide activity against three insect pests (LD{sub 50} from 2.72 to 39.71 mg g{sup -1} a.i.). The increasing order of insects susceptibility to coumarin was R. dominica, S. zeamais and O. surinamensis. (author)

  8. Plant compounds insecticide activity against Coleoptera pests of stored products

    International Nuclear Information System (INIS)

    Moreira, Marcio Dionizio; Picanco, Marcelo Coutinho; Guedes, Raul Narciso Carvalho; Campos, Mateus Ribeiro de; Silva, Gerson Adriano; Martins, Julio Claudio; julioufv@yahoo.com.br

    2007-01-01

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on R. dominica was evaluated. Among them, only hexane extract of A. conyzoides showed insecticide activity; the hexane extract of this species was successively fractionated by silica gel column chromatography, for isolation and purification of the active compounds. Compounds 5,6,7,8,3',4',5'-heptamethoxyflavone; 5,6,7,8,3'-pentamethoxy-4',5'-methilenedioxyflavone and coumarin were identified. However, only coumarin showed insecticide activity against three insect pests (LD 50 from 2.72 to 39.71 mg g -1 a.i.). The increasing order of insects susceptibility to coumarin was R. dominica, S. zeamais and O. surinamensis. (author)

  9. Diversity of Scydmaeninae (Coleoptera: Staphylinidae) in Upper Eocene Rovno amber.

    Science.gov (United States)

    Jałoszyński, Paweł; Perkovsky, Evgeny

    2016-08-25

    Among nearly 1270 inclusions of Coleoptera found in Upper Eocene Rovno amber, 69 were identified as ant-like stone beetles (Scydmaeninae); 34 were possible to unambiguously determine to the tribal level and were studied in detail. Rovnoleptochromus ableptonoides gen. & sp. n. (Mastigitae: Clidicini), Vertheia quadrisetosa gen. & sp. n. (Cephenniitae: Eutheiini), Cephennomicrus giganteus sp. n. (Cephenniitae: Cephenniini), Glaesoconnus unicus gen. & sp. n. (Scydmaenitae: Glandulariini), Rovnoscydmus frontalis gen. & sp. n. (Scydmaenitae: Glandulariini; type species of Rovnoscydmus), Rovnoscydmus microscopicus sp. n., Euconnus (incertae sedis, near Cladoconnus) palaeogenus sp. n. (Scydmaenitae: Glandulariini), and Stenichnus (s. str.) proavus sp. n. (Scydmaenitae: Glandulariini) are described. Additionally, specimens representing one undescribed species of Vertheia, one of Cephennodes, five of Cephennomicrus, one of Euconnus, one of Microscydmus are recorded, and nine specimens representing an unknown number of species of Rovnoscydmus (and two putative Rovnoscydmus), one Euconnus (and one putative Euconnus), two putative Microscydmus and one putative Scydmoraphes were found in the studied material. The composition of Scydmaeninae fauna in Rovno amber is discussed in the context of ecological preferences and distribution of extant taxa. It is concluded that subtropical and tropical taxa were present in the region where Rovno amber has formed, most notably the second genus and species of the extant tribe Clidicini known from the Eocene of Europe, and six species of the extant genus Cephennomicrus, for the first time found in the fossil record. An annotated catalog of nominal species of Scydmaeninae known in the fossil record is given.

  10. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Sri Puji Astuti Wahyuningsih

    2016-03-01

    Full Text Available Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1 and adding polysaccharide krestin before-after exposure (P3 had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2 had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1, 12-17.

  11. Emergence of Buprestidae, Cerambycidae, and Scolytinae (Coleoptera) from mountain pine beetle-killed and fire-killed ponderosa pines in the Black Hills, South Dakota, USA

    Science.gov (United States)

    Sheryl L. Costello; William R. Jacobi; Jose F. Negron

    2013-01-01

    Wood borers (Coleoptera: Cerambycidae and Buprestidae) and bark beetles (Coleoptera: Curculionidae) infest ponderosa pines, Pinus ponderosa P. Lawson and C. Lawson, killed by mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, and fire. No data is available comparing wood borer and bark beetle densities or species guilds associated with MPB-killed or fire-...

  12. Treatment of pityriasis versicolor with topical application of essential oil of Cymbopogon citratus (DC Stapf - therapeutic pilot study Tratamento de pitiríase versicolor com aplicação tópica do óleo essencial de Cymbopogon citratus (DC Stapf - estudo terapêutico piloto

    Directory of Open Access Journals (Sweden)

    Egberto Santos Carmo

    2013-06-01

    Full Text Available BACKGROUND: Pityriasis versicolor is a fungal infection caused by Malassezia spp. that has frequent relapses. OBJECTIVES: The main objective of this research was to perform phase I and II clinical studies, using formulations containing essential oil of Cymbopogon citratus in patients with pityriasis versicolor. METHODS: Phase I study included twenty volunteers to ascertain the safety of the formulations. In phase II, 47 volunteers randomly received essential oil formulations at 1.25 μL/mL concentration, for forty days. The shampoo should be applied three times a week and the cream twice a day. A control group in phase II, consisting of 29 volunteers, received the same formulations but with 2% ketoconazole as the active ingredient. RESULTS: No significant adverse events were observed in volunteers during Phase I. In Phase II, 30 (63.83% volunteers using essential oil and 18 (62.07% using ketoconazole remained until the end of the study. We observed a predominance of lesions in disseminated form, with M. sympodialis detected as the predominant agent identified in cultures. After 40 days of treatment, the rate of mycological cure was 60% (p FUNDAMENTOS: Pitiríase versicolor é uma micose causada pela Malassezia spp., e que apresenta frequentes recidivas. OBJETIVOS: Este trabalho objetivou realizar estudos clínicos de fase I e II, para essa patologia, com óleo essencial de Cymbopogon citratus. MÉTODOS: Na fase I, participaram vinte voluntários para averiguar a segurança das formulações. Na fase II, 47 voluntários receberam as formulações do óleo essencial a 1,25 μL/mL, as quais deveriam ser utilizadas por quarenta dias, sendo o xampu três vezes por semana e o creme duas vezes ao dia. Um grupo controle na fase II, constituído por 29 voluntários recebeu as mesmas formulações, com cetoconazol a 2%. RESULTADOS: Verificada a segurança das formulações com a finalização da fase I, onde nenhuma reação adversa significativa foi

  13. Biocontrol of larval mosquitoes by Acilius sulcatus (Coleoptera: Dytiscidae

    Directory of Open Access Journals (Sweden)

    Banerjee Siddhartha S

    2008-10-01

    Full Text Available Abstract Background Problems associated with resistant mosquitoes and the effects on non-target species by chemicals, evoke a reason to find alternative methods to control mosquitoes, like the use of natural predators. In this regard, aquatic coleopterans have been explored less compared to other insect predators. In the present study, an evaluation of the role of the larvae of Acilius sulcatus Linnaeus 1758 (Coleoptera: Dytiscidae as predator of mosquito immatures was made in the laboratory. Its efficacy under field condition was also determined to emphasize its potential as bio-control agent of mosquitoes. Methods In the laboratory, the predation potential of the larvae of A. sulcatus was assessed using the larvae of Culex quinquefasciatus Say 1823 (Diptera: Culicidae as prey at varying predator and prey densities and available space. Under field conditions, the effectiveness of the larvae of A. sulcatus was evaluated through augmentative release in ten cemented tanks hosting immatures of different mosquito species at varying density. The dip density changes in the mosquito immatures were used as indicator for the effectiveness of A. sulcatus larvae. Results A single larva of A. sulcatus consumed on an average 34 IV instar larvae of Cx. quinquefasciatus in a 24 h period. It was observed that feeding rate of A. sulcatus did not differ between the light-on (6 a.m. – 6 p.m., and dark (6 p.m. – 6 a.m. phases, but decreased with the volume of water i.e., space availability. The prey consumption of the larvae of A. sulcatus differed significantly (P A. sulcatus larvae, while with the withdrawal, a significant increase (p A. sulcatus in regulating mosquito immatures. In the control tanks, mean larval density did not differ (p > 0.05 throughout the study period. Conclusion the larvae of the dytiscid beetle A. sulcatus proved to be an efficient predator of mosquito immatures and may be useful in biocontrol of medically important mosquitoes.

  14. Using malaise traps to sample ground beetles (Coleoptera: Carabidae).

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D., James L. Hanula, and Scott Horn

    2005-01-01

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  15. Using malaise traps to sample ground beetles (Coleoptera. Carabidae)

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Hanula, James L. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Horn, Scott [USDA Forest Service, Savannah River, New Ellenton, SC (United States)

    2012-04-02

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  16. Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae

    Directory of Open Access Journals (Sweden)

    Elias Soares Gomes

    2014-06-01

    Full Text Available Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae. Coleopterans of the family Melolonthidae comprise a large group of species that feed on different food sources, including plant roots, stems, and leaves, in addition to plant materials at different decomposition stages. Several species are found in the genus Leucothyreus, occurring in different regions of Brazil, including the various biomes in the country. Information on the biology of species of the genus Leucothyreus is scarce, therefore, we conducted studies on the biological aspects of Leucothyreus ambrosius Blanchard, 1850. The period of adult occurrence was determined with a light trap installed between a cropped and pasture area in the municipality of Aquidauana, Mato Grosso do Sul State, Brazil. Adults collected in the field were used to form insect pairs and the studies were initiated in the entomology laboratory as the adults began ovipositing. Adults were observed flying in the field from October to December. Eggs were obtained as pairs were formed and a colony was established, the embryonic period lasting 14.6 days on average. The larval period in the 1st instar lasted 21.6 days, in the 2nd instar 19.6 days, and in the 3rd instar, 85.6 days. The head capsule width was 1.48 mm in the 1st instar, 2.44 mm in the 2nd, and 3.83 mm in 3rd larval instar. The pupal stage had an average duration of 35.5 days. The egg to adult period lasted 173.3 days. Morphometric information for the larval and adult stages is presented in this study.

  17. ESPECIES DE DRYOPHTHORINAE (COLEOPTERA: CURCULIONIDAE ASOCIADAS A PLÁTANO Y BANANO (Musa spp. EN COLOMBIA

    Directory of Open Access Journals (Sweden)

    JOSE RUBIO-GOMEZ

    2009-05-01

    Full Text Available RESUMEN Se presenta una sinopsis de los escarabajos de la subfamilia Dryophthorinae (Coleoptera: Curculionidae asociados a cultivos de plátano y banano en Colombia. Adicionalmente se ofrecen claves ilustradas para las especies del país. Se registran seis especies asociadas a dichos cultivos: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838. Palabras clave: picudos, plátano, banano, Polytus, Colombia. ABSTRACT This synopsis is about beetle’s subfamily Dryophthorinae (Coleoptera: Curculionidae associated to plantain and banana crops. Additionally keys illustrated for the species of the country are offered. Six species associated to these cultures are registered: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838. Key words: weevils, plantain, banana, Polytus, Colombia.

  18. Species of beetles (Coleoptera; Scarabaeidae associated to banana (Musa spp. in Ceballos, Ciego de Avila, Cuba

    Directory of Open Access Journals (Sweden)

    Maria Luisa Sisne Luis

    2016-03-01

    Full Text Available A white light trap was placed in bananas plantations, according to Sisne, 2009 and MINAG, 1985, in the Citric enterprise of Ciego de Ávila during the period between May and July of 2010 with the objective of determining the composition of genus and species of the order Coleoptera family Scarabaeidae associated to the agroecosystem. The species Cyclocephala cubana Chapin, Phyllophaga puberula Duval, and Phyllophaga patruelis Chev. are associated to bananas crops in these areas.

  19. EFFECTS OF WEEVILS (INSECTA: COLEOPTERA: CURCULIONIDAE) CONTROL PRODUCTS, OVER THE GROWTH, DEVELOPMENT, AND PRODUCTION OF PLANTAIN

    OpenAIRE

    M. Muñoz, Liliana; Cañas, Guillermo L.; Urrea, Aura I.; Guarín, Juan H.

    2013-01-01

    In a farm in the municipality of Andes (Antioquia, Colombia), parcels were planted with Dominico Hartón plantain associated with Caturra-type coffee, where weevil damage (Insecta: Coleoptera: Curculionidae) occurred in 100% of the plantain plants, corms of approximately 2 kg were planted under the same association system. From the sowing until harvest, six types of products were applied every two months on these plantations: chemical of the region (Clorpirifos and Carboxin + Thiram), Carbofur...

  20. Dryophthorinae Species (Coleoptera: Curculionidae) Associted to Platain and Banana crops (Musaspp.) in Colombia

    OpenAIRE

    SEPÚLVEDA-CANO, PAULA A; RUBIO-GÓMEZ, JOSÉ D

    2009-01-01

    Se presenta una sinopsis de los escarabajos de la subfamilia Dryophthorinae (Coleoptera: Curculionidae) asociados a cultivos de plátano y banano en Colombia. Adicionalmente se ofrecen claves ilustradas para las especies del país. Se registran seis especies asociadas a dichos cultivos: Cosmopolites sordidus (Germar, 1824), Metamasius hemipterus (Linnaeus, 1758), Metamasius hebetatus (Gyllenhal, 1838), Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758) y Polytus mell...

  1. A new Icimauna Martins & Galileo, 1991, from the Bolivian orocline (Coleoptera, Cerambycidae, Lamiinae, Hemilophini).

    Science.gov (United States)

    Santos-Silva, Antonio; Perger, Robert

    2017-04-07

    The Neotropical longhorned beetle tribe Hemilophini has been reviewed by Martins & Galileo (2014a, b) and currently contains 542 species (Monné 2017). Some of the most conspicuous longhorned beetle taxa are found in this tribe, for example species with a pair of cephalic horns (Phoebe Audinet-Serville, 1835), or others that strongly resemble to noxious Lycidae (Coleoptera) (e.g. Apeba Martins & Galileo, 1991, Calocosmus Chevrolat, 1862, or Lycidola Thomson, 1864) (see Lingafelter 2013; Martins & Galileo 2014a, b).

  2. Novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae da Bolívia

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2008-01-01

    Full Text Available Novas espécies descritas da Bolívia, Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.New species of Onciderini (Coleoptera, Cerambycidae, Lamiinae from Bolivia. New species described from Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.

  3. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern

    Directory of Open Access Journals (Sweden)

    SUGIYARTO

    2009-07-01

    Full Text Available Maryati KT, Sugiyarto. 2010. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern. Nusantara Bioscience 1: 72-77. This research aims to find out the white grub (Melolonthidae; Coleoptera variability based on the morphological characteristic and protein banding pattern found in ”salak pondoh” farm in Regencies of Sleman, Yogyakarta and Magelang, Central Java. Each area has five sampling points. Morphological analysis on white grub was conducted using descriptive method and analysis on protein banding pattern was conducted using qualitative analysis based on the presence or absent of band pattern on the gel, and qualitatively based on the relative mobility value (Rf of protein. The result indicated that the white grub in Sleman and Magelang, based on morphology characteristic is only one species, namely Holothricia sp. Based on the protein banding pattern, the white grub sample have differences of protein band number and protein molecular weight. Key words: Salacca zalacca, white grub, morphology, protein banding pattern.Abstrak. Maryati KT, Sugiyarto. 2010. Karakterisasi lundi putih (Melolonthidae: Coleoptera pada pertanaman salak berdasarkan ciri morfologi dan pola pita protein. Nusantara Bioscience 1: 72-77. Penelitian ini bertujuan untuk mengetahui keanekaragaman lundi putih (Melolonthidae; Coleoptera berdasarkan ciri morfologi dan pola pita protein yang ditemukan di lahan pertanaman salak pondoh di Kabupaten Sleman, Yogyakarta dan Kabupaten Magelang, Jawa Tengah. Pada masing-masing wilayah diambil lima titik sampling. Analisis morfologi lundi putih digunakan metode deskriptif, dan analisis pola pita protein digunakan analisis kualitatif berdasarkan muncul tidaknya pola pita pada gel, dan secara kuantitatif berdasarkan nilai mobilitas relatif protein (RF. Hasil penelitian menunjukkan bahwa sampel lundi putih di Kabupaten Sleman dan Magelang, berdasar karakter

  4. Fine fluorescent powder marking study of dispersal in the spruce bark beetle, Ips typographus (Coleoptera: Scolytidae)

    Czech Academy of Sciences Publication Activity Database

    Doležal, Petr; Okrouhlík, Jan; Davídková, Markéta

    2016-01-01

    Roč. 113, JAN 07 (2016), s. 1-8 E-ISSN 1802-8829 Grant - others:Forests of the Czech Republic(CZ) 08/2009; MŠMT(CZ) LH12098 Institutional support: RVO:60077344 Keywords : Coleoptera * Scolytidae * Ips typographus Subject RIV: EH - Ecology, Behaviour Impact factor: 1.167, year: 2016 http://www.eje.cz/pdfs/eje/2016/01/01.pdf

  5. Otiorhynchus spp. (Coleoptera: Curculionidae) as pests in horticulture: genetics and management options with entomopathogenic fungi

    OpenAIRE

    Hirsch, Jacqueline

    2012-01-01

    Worldwide, weevils of the genus Otiorhynchus (Coleoptera: Curculionidae) cause damage with detrimental economic effects to many horticultural crops due to the root feeding of their larvae as well as foliage feeding of their adults. Aside from the black vine weevil Otiorhynchus sulcatus, which is the best-known pest within this genus, numerous other Otiorhynchus species have been increasingly recognized as pests in recent years. Nocturnal adult weevils and soil-inhibiting larvae are in princip...

  6. A checklist of the genus Blosyrus Schoenherr (Coleoptera: Curculionidae: Entiminae of the world

    Directory of Open Access Journals (Sweden)

    G. Mahendiran

    2013-08-01

    Full Text Available An annotated checklist of Blosyrus Schoenherr (Coleoptera: Curculionidae: Entiminae comprising 101 species with their updated nomenclature, synonyms and distribution is given. The distribution pattern indicates that the genus is diversified mostly in the Sub-Saharan Africa and Asia regions. Out of 101 species, 92 occur in Sub-Saharan Africa. From India, eight species were recorded. In India, the distribution is mainly in West Bengal, Assam, Maharashtra, Andhra Pradesh, Karnataka and Tamil Nadu.

  7. Aquatic Coleoptera assemblages in protected wetlands of North-western Spain

    Directory of Open Access Journals (Sweden)

    Amaia Pérez-Bilbao

    2014-02-01

    Full Text Available Wetlands are diverse and productive ecosystems endangered by human pressure, which degradation implies a biodiversity loss worldwide. Among the biological assemblages of these habitats, aquatic Coleoptera is one of the most diverse and useful groups when assessing the ecological conditions of the ecosystems they inhabit. The aims of the present study were to analyze the diversity and composition of aquatic Coleoptera assemblages in 24 wetlands protected by the Natura 2000 network of North-western Spain and the influence of environmental variables on the distribution of species, in order to detect differences between the different types of standing water habitats. A total of 11,136 individuals of 105 species belonging to 12 families of aquatic Coleoptera (Gyrinidae, Haliplidae, Noteridae, Paelobiidae, Dytiscidae, Helophoridae, Hydrochidae, Hydrophilidae, Hydraenidae, Scirtidae, Elmidae and Dryopidae were collected. In general, wetlands presented high richness and diversity values, Dytiscidae and Hydrophilidae having the highest species richness. Most of recorded species have a wide biogeographical distribution and only 12 endemic ones were captured. Cluster and Non-Metric Multi-Dimensional Scaling (NMDS analyses showed the clustering of the studied ponds and lagoons in four groups based on biological data. In general, the wetlands of each group seem to have distinct aquatic Coleoptera faunas, as showed by the most representative species. A combination of altitude, SST and hydroperiod was the best explaining factor of the distribution of the species throughout the study area. This study shows the high biodiversity of standing water habitats in North-western Spain and the usefulness of water beetles in establishing habitat typologies.

  8. Distribution of the longhorned beetle Callipogon relictus (Coleoptera: Cerambycidae) in Northeast Asia.

    Science.gov (United States)

    Yi, Dae-Am; Kuprin, Alexander V; Bae, Yeon Jae

    2018-01-02

    The distribution of the longhorned beetle Callipogon (Eoxenus) relictus Semenov, 1899 (Coleoptera: Cerambycidae) in Northeast Asia was summarized for the first time, based on the examination of comprehensive material. Callipogon relictus is the only representative of the genus Callipogon in the Palearctic region. Its distribution ranges from Shanxi province (China) in the south, northward through the Korean Peninsula to Amur Oblast (Russia), and from Inner Mongolia (China) in the west to the coastal area of the Russian Far East.

  9. New faunistic records of ground beetles (Coleoptera, Carabidae from Hormozgan province, Iran

    Directory of Open Access Journals (Sweden)

    Azadbakhsh Saeed

    2016-09-01

    Full Text Available This paper presents the results of a faunal study of ground beetles (Coleoptera: Carabidae from Hormozgan province in southern Iran, which was carried out from winter 2015 to winter 2016. A total of 30 species belonging to 18 genera were collected and identified. Two species – Calodromius mayeti and Elaphropus (Tachyura biblis – are reported from Iran for the first time; in addition, the occurrence of several species in Iran was confirmed.

  10. New distribution record of Cybocephalus kathrynae (Coleoptera, Cybocephalidae on Mona Island, Puerto Rico

    Directory of Open Access Journals (Sweden)

    Jean Carlos Curbelo-Rodríguez

    2012-03-01

    Full Text Available New distribution record of Cybocephalus Kathrynae (Coleoptera, Cybocephalidae on Mona Island, Puerto Rico. A new record of Cybocephalus kathrynae T.R. Smith (Cybocephalidae is reported for Puerto Rico. Adults were collected from the flowers of Mammillaria nivosa (Cactaceae on Mona Island Reserve. Prior to this study, this beetle species was only reported for Monroe and Miami-Dade Counties, Florida, USA.

  11. Significantly higher Carabid beetle (Coleoptera: Carabidae) catch in conventionally than in organically managed Christmas tree plantations

    DEFF Research Database (Denmark)

    Bagge, Søren; Lund, Malthe; Rønn, Regin

    2012-01-01

    Carabid beetles play an important role as consumers of pest organisms in forestry and agriculture. Application of pesticides may negatively affect abundance and activity of carabid beetles, thus reducing their potential beneficial effect. We investigated how abundance and diversity of pitfall...... trapped carabid beetles (Coleoptera, Carabidae) varied between conventionally and organically managed Caucasian Fir (Abies nordmanniana (Stev.)) plantations, in northern Zealand, Denmark. We recorded significantly higher numbers of carabid beetle specimens and species at conventionally than at organically...

  12. An annotated synopsis of the powder post beetles of Iran (Coleoptera: Bostrichoidea: Bostrichidae

    Directory of Open Access Journals (Sweden)

    Lan-Yu Liu

    2016-07-01

    Full Text Available An annotated synopsis of Iranian Bostrichidae (Coleoptera: Bostrichoidea is provided as a basis for future studies, with notes on distribution, host plants, biology and economic importance. In total, 31 species from 18 genera and 4 subfamilies (Bostrichinae, Dinoderinae, Lyctinae and Psoinae are listed from Iran. Sinoxylon anale Lesne, 1897, Sinoxylon perforans (Schrank, 1789, Stephanopachys linearis (Kugelann, 1792 and Xylopertha retusa (Olivier, 1790 are new records for Iran.

  13. Molecular Markers Detect Cryptic Predation on Coffee Berry Borer (Coleoptera: Curculionidae) by Silvanid and Laemophloeid Flat Bark Beetles (Coleoptera: Silvanidae, Laemophloeidae) in Coffee Beans.

    Science.gov (United States)

    Sim, Sheina B; Yoneishi, Nicole M; Brill, Eva; Geib, Scott M; Follett, Peter A

    2016-02-01

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is a serious pest of coffee worldwide. It was first detected in Hawai'i in 2010. Two predatory beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and Leptophloeus sp. (Coleoptera: Laemophloeidae), have been observed in H. hampei-infested coffee. Under laboratory conditions, colony-reared C. quadricollis and Leptophloeus sp. prey upon all life stages of H. hampei. However, the H. hampei life cycle occurs almost exclusively within a coffee bean obscured from direct observation. Thus, it is unknown if C. quadricollis and Leptophloeus sp. consume H. hampei as prey in the wild. To demonstrate predation of H. hampei by C. quadricollis and Leptophloeus sp., a molecular assay was developed utilizing species-specific primers targeting short regions of the mitochondrial COI gene to determine species presence. Using these primers, wild C. quadricollis and Leptophloeus sp. were collected and screened for the presence of H. hampei DNA using PCR. Analysis of collections from five coffee farms revealed predation of C. quadricollis and Leptophloeus sp. on H. hampei. Further laboratory testing showed that H. hampei DNA could be detected in predators for as long as 48 h after feeding, indicating the farm-caught predators had preyed on H. hampei within 2 d of sampling. This study demonstrates the utility of molecular markers for the study of the ecology of predators and prey with cryptic behavior, and suggests C. quadricollis and Leptophloeus sp. might be useful biocontrol agents against H. hampei. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  14. Nuevas citas de Coleoptera acuáticos y Megaloptera para la provincia de Chubut (Argentina New records of aquatic Coleoptera and Megaloptera from Chubut province (Argentina

    Directory of Open Access Journals (Sweden)

    Miguel Archangelsky

    2004-12-01

    Full Text Available Se informa acerca de nuevos hallazgos de coleópteros acuáticos, de Dytiscidae, Gyrinidae, Hydrophilidae y Elmidae para la provincia de Chubut (Argentina. También se cita por primera vez a las Sialidae (Megaloptera, género Protosialis Weele, para la República Argentina.New records of aquatic Coleoptera, in the families Dytiscidae, Gyrinidae, Hydrophilidae and Elmidae, are reported for the Chubut province (Argentina. The Sialidae (Megaloptera, genus Protosialis Weele, is reported for the first time in Argentina.

  15. Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)

    Science.gov (United States)

    Nenadić, Marija; Soković, Marina; Glamočlija, Jasmina; Ćirić, Ana; Perić-Mataruga, Vesna; Ilijin, Larisa; Tešević, Vele; Vujisić, Ljubodrag; Todosijević, Marina; Vesović, Nikola; Ćurčić, Srećko

    2016-04-01

    The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens—eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var . cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var . cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.

  16. Ocorrência de Migdolus fryanus (Coleoptera: Cerambycidae em plantios de Pinus caribaea var. hondurensis Occurrence of Migdolus fryanus (Coleoptera: Cerambycidae in Pinus caribaea hondurensis plantations

    Directory of Open Access Journals (Sweden)

    Carlos Frederico Wilcken

    2005-02-01

    Full Text Available Larvas de Migdolus fryanus Westwood (Coleoptera: Cerambycidae foram encontradas danificando raízes de mudas de P. caribaea var. hondurensis (Sénéel Barr. & Golf. no Estado de São Paulo. Isso aumenta a importância dessa espécie, cujos danos, em espécies florestais, têm aumentado, principalmente, em plantios de eucalipto. Esse é o primeiro registro de M. fryanus em plantios de Pinus, e o referido inseto pode ser considerado uma nova praga dessa espécie florestal. Detalhes das características morfológicas e biológicas, danos e possíveis métodos de controle de M. fryanus são discutidos.Larvae of Migdolus fryanus Westwood (Coleoptera: Cerambycidae were found damaging roots of young plants of P. caribaea hondurensis (Sénéel Barr. & Golf. in the State of São Paulo, Brazil. This fact increases the importance of this species because the damages have been increasing in forest species specially in eucalyptus plantations. This is the first record of M. fryanus in pine plantations and this insect can be considered a pest of pine plants. Details on the morphology and biological characteristics, damage and possible control methods to M. fryanus are discussed.

  17. Enhanced biodegradation of antibiotic combinations via the sequential treatment of the sludge resulting from pharmaceutical wastewater treatment using white-rot fungi Trametes versicolor and Bjerkandera adusta.

    Science.gov (United States)

    Aydin, Sevcan

    2016-07-01

    While anaerobic treatment is capable of treating pharmaceutical wastewater and removing antibiotics in liquid phases, solid phases may still contain significant amounts of antibiotics following this treatment. The main goal of this study was to evaluate the use of white-rot fungi to remove erythromycin, sulfamethoxazole, and tetracycline combinations from biosolids. The degradation potential of Trametes versicolor and Bjerkandera adusta was evaluated via the sequential treatment of anaerobic sludge. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses were used to identify competition between the autochthonous microbial communities and white-rot fungi. Solid-phase treatment using white-rot fungi substantially reduced antibiotic concentrations and toxicity in sludge. According to PCR-DGGE results, there is an association between species of fungus and antibiotic type as a result of the different transformation pathways of fungal strains. Fungal post-treatment of sludge represents a promising method of removing antibiotic combinations, therefore holding a significant promise as an environmentally friendly means of degrading the antibiotics present in sludge.

  18. Oxidation of atenolol, propranolol, carbamazepine and clofibric acid by a biological Fenton-like system mediated by the white-rot fungus Trametes versicolor.

    Science.gov (United States)

    Marco-Urrea, Ernest; Radjenović, Jelena; Caminal, Gloria; Petrović, Mira; Vicent, Teresa; Barceló, Damià

    2010-01-01

    Biological advanced oxidation of the pharmaceuticals clofibric acid (CA), carbamazepine (CBZP), atenolol (ATL) and propranolol (PPL) is reported for the first time. Extracellular oxidizing species were produced through a quinone redox cycling mechanism catalyzed by an intracellular quinone reductase and any of the ligninolytic enzymes of Trametes versicolor after addition of the lignin-derived quinone 2,6-dimethoxy-1,4-benzoquinone (DBQ) and Fe(3+)-oxalate in the medium. Time-course experiments with approximately 10mg L(-1) of initial pharmaceutical concentration resulted in percent degradations above 80% after 6h of incubation. Oxidation of pharmaceuticals was only observed under DBQ redox cycling conditions. A similar degradation pattern was observed when CBZP was added at the environmentally relevant concentration of 50 microg L(-1). Depletion of DBQ due to the attack of oxidizing agents was assumed to be the main limiting factor of pharmaceutical degradation. The main degradation products, that resulted to be pharmaceutical hydroxylated derivatives, were structurally elucidated. The detected 4- and 7-hydroxycarbamazepine intermediates of CBZP degradation were not reported to date. Total disappearance of intermediates was observed in all the experiments at the end of the incubation period. (c) 2009 Elsevier Ltd. All rights reserved.

  19. Abridged life tables for Cephalonomia stephanoderis and Prorops nasuta (Hymenoptera: Bethylidae) Parasitoids of Hypothenemus hampei (Coleoptera: Scolytidae) reared on artificial diet

    Science.gov (United States)

    Biological aspects and demographic parameters of Cephalonomia stephanoderis Betrem (Hymenoptera: Bethylidae) and Prorops nasuta Waterston (Hymenoptera: Bethylidae) parasitoids of the coffee berry borer (CBB), Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) were investigated usi...

  20. Monitoring the establishment and flight phenology of parasitoids of emerald ash borer (Coleoptera: Buprestidae) in Michigan by using sentinel eggs and larvae

    Science.gov (United States)

    Kristopher J. Abell; Leah S. Bauer; Deborah L. Miller; Jian J. Duan; Roy G. Van Driesche

    2016-01-01

    The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is an important invasive pest of ash (Fraxinus) trees in North America. Two larval parasitoid species, Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) and Spathius agrili Yang (Hymenoptera:...

  1. Estudo da descoloração do corante FD&C azul no 2 Indigotina pelo tratamento combinado do fungo Trametes versicolor e processo de filtração lenta

    Directory of Open Access Journals (Sweden)

    Maria Margareth Gonçalves Lopes

    2014-06-01

    Full Text Available O uso de fungos na descoloração de corantes com métodos economicamente viáveis de produção de água bacteriologicamente segura há muito vem sendo descrito por diversos autores. Este trabalho teve por objetivo investigar a eficiência da remoção de corante artificial FD&C azul no 2 Indigotina, com uso do fungo de degradação branca Trametes versicolor em combinação com a filtração lenta. Para a realização dos trabalhos, foram instalados dois protótipos de filtros lentos denominados FL-A e FL-B - no sobrenadante do filtro FL-A foi inoculado o referido fungo, e o filtro FL-B foi utilizado como controle (sem inoculação do microrganismo. O melhor percentual de remoção do corante pelo fungo Trametes versicolor em combinação com a filtração lenta foi de 44,74% 24 horas após a atividade máxima registrada de lacase. Os resultados mostraram que a filtração lenta combinada com o tratamento com o fungo T. versicolor não apresenta grande potencial para remoção de cor em 21 dias de tratamento, visto que os produtos microbianos gerados interferem no processo de filtração, diminuindo a eficiência do processo físico. Entretanto, restringindo o tempo de tratamento a 24 horas após a atividade enzimática máxima, o tratamento combinado apresentou boa eficiência.

  2. Responses of Ips pini (Say), Pityogenes knechteli Swaine and Associated Beetles (Coleoptera) to Host Monoterpenes in Stands of Lodgepole Pine

    Science.gov (United States)

    Daniel R. Miller; John H. Borden

    2003-01-01

    We conducted seven experiments in stands of mature lodgepole pine in southern British Columbia to elucidate the role of host volatiles in the semiochemical ecology of the pine engraver, Ips pini (Say) (Coleoptera: Scolytidae), with particular reference to the behavioral responses of predators and competing species of bark beetles. Our results demonstrated that the...

  3. Development of a satellite-based hazard rating system for Dendrctonus frontallis (Coleoptera: Scolytidae) in the Ouachita Mountains of Arkansas

    Science.gov (United States)

    Stephen Cook; Shane Cherry; Karen Humes; James Guldin; Christopher Williams

    2007-01-01

    The southern pine beetle, Dendroctonus frontalis Zimmermann (Coleoptera: Scolytidae), is the most damaging forest insect pest of pines (Pinus spp.) throughout the southeastern United States. Hazard rating schemes have been developed for D. frontalis, but for these schemes to be accurate and effective, they...

  4. Comparison of trap types, placement, and colors for monitoring Anthonomus musculus (Coleoptera: Curculionidae) adults in highbush blueberries

    Science.gov (United States)

    Cranberry weevil, Anthonomus musculus Say (Coleoptera: Curculionidae), is a key pest of highbush blueberries in the northeast USA. To date, however, no trapping system has been developed to successfully monitor this pest. In 2012-2014, studies were conducted in commercial blueberry farms in New Jers...

  5. Attraction of Agrilus planipennis (Coleoptera: Buprestidae) and other buprestids to sticky traps of various colors and shapes

    Science.gov (United States)

    Toby R. Petrice; Robert A. Haack; Therese M. Poland

    2013-01-01

    The family Buprestidae (Coleoptera) contains numerous economically significant species, including the emerald ash borer (EAB), Agrilus planipennis Fairmaire, first discovered in North America in 2002. Effective traps for monitoring spread and population densities of EAB and other buprestids are needed. Studies were conducted in 2008 to test different...

  6. Podisus distinctus (Heteroptera: Pentatomidae) females are lighter feeding on Tenebrio molitor (Coleoptera: Tenebrionidae) pupae subjected to ventral nerve cord transection

    Science.gov (United States)

    The movement observed in the Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae) pupae can be a type of defense strategy. This makes it significant to study the development and reproduction of the predatory stinkbugs Asopinae with the immobilized pupae of this prey. The aim was to evaluate the per...

  7. Self-selection of two diet components by Tennebrio molitor (Coleoptera: Tenebrionidae) larvae and its impact on fitness

    Science.gov (United States)

    We studied the ability of Tenebrio molitor L. (Coleoptera: Tenebrionidae) to self-select optimal ratios of two dietary components to approach nutritional balance and maximum fitness. Life table analysis was used to determine the fitness of T. molitor developing in diet mixtures comprised of four dif...

  8. Developmental plasticity in Tenebrio molitor (Coleoptera: Tenebrionidae): Analysis of Instar Variation in Number and Development Time under Different Diets

    Science.gov (United States)

    The variation in instar number and the pattern of sequential instar development time of Tenebrio molitor L. (Coleoptera: Tenebrionidae) was studied under 4 different diet regimes. Addition of dietary supplements consisting of dry potato or a mix of dry potato and dry egg whites significantly reduced...

  9. Biology and life history of Argopistes tsekooni (Coleoptera: Chrysomelidae) in China, a promising biological control agent of Chinese privet.

    Science.gov (United States)

    Y-Z Zhang; J. Sun; J.L. Hanula

    2009-01-01

    The biology and life history of Argopistes tsekooni Chen (Coleoptera: Chrysomelidae), a potential biological control agent of Chinese privet, Ligustrum sinense Lour., was studied under laboratory and outdoor conditions in Huangshan City of Anhui Province, China, in 2006. A. tsekooni larvae are leafminers that...

  10. Flight periodicity of the Douglas-fir beetle, Dendroctonus pseudotsugae Hopkins (Coleoptera: Curculionidae: Scolytinae) in Colorado, U.S.A

    Science.gov (United States)

    Jose F. Negron; Willis C. Schaupp; Lee Pederson

    2011-01-01

    There are about 500 species of bark beetles (Coleoptera: Curculionidae: Scolytinae) in the United States (Wood 1982). A number of them are important disturbance agents in forested ecosystems, occasionally creating large tracts of dead trees. One eruptive species is the Douglas-fir beetle, Dendroctonus pseudotsugae Hopkins, which utilizes Douglas-fir, Pseudotsuga...

  11. Evaluating the use of plastic bags to prevent escape of the emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae) from firewood

    Science.gov (United States)

    Therese M. Poland; Tina M. Ciaramitaro; Deepa S. Pureswaran; Andrea Diss-Torrance

    2008-01-01

    The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a highly destructive exotic pest of ash (Fraxinus) in North America. Human movement of infested logs, primarily pieces of firewood, is a major pathway for long distance spread of the beetle. Firewood may be confiscated at campgrounds, rest-areas, and...

  12. Biology and natural enemies of Agrilus fleischeri (Coleoptera:Buprestidae), a newly emerging destructive buprestid pest in Northeast China

    Science.gov (United States)

    The jewel beetle Agrilus fleischeri Obenberger (Coleoptera: Buprestidae) is a newly emerging major pest of poplar trees (Populus spp.) in northeast China and is responsible for the poplar mortality throughout its distribution range. In order to determine how to manage this pest effectively, we stud...

  13. Microbial control of emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae) with Beauveria bassiana strain GHA: Greenhouse and field trials

    Science.gov (United States)

    Houping Liu; Leah S. Bauer

    2008-01-01

    In 2003-2004, the lethal and sublethal effects of Beauveria bassiana strain GHA on emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae) adults and larvae were evaluated using topical spray and fungal band treatments in the greenhouse and field. B. bassiana strain GHA was moderately effective against...

  14. Influence of elevation on bark beetle (Coleoptera: Curculionidae, Scolytinae) community structure and flight periodicity in ponderosa pine forests of Arizona

    Science.gov (United States)

    Kelly K. Williams; Joel D. McMillin; Tom E. DeGomez; Karen M. Clancy; Andy Miller

    2008-01-01

    We examined abundance and flight periodicity of five Ips and six Dendroctonus species (Coleoptera: Curculionidae, Scolytinae) among three different elevation bands in ponderosa pine (Pinus ponderosa Douglas ex. Lawson) forests of northcentral Arizona. Bark beetle populations were monitored at 10 sites in each of three elevation...

  15. Nuevos datos de distribución de los Cholevinae hipogeos del Atlas marroquí (Coleoptera, Leiodidae

    Directory of Open Access Journals (Sweden)

    Fresneda, J.

    2006-12-01

    Full Text Available New distribution data for the hypogean Cholevinae from the Moroccan Atlas (Coleoptera, Leiodidae The authors report new findings on the distribution of Speonemadus maroccanus (Jeannel, 1936, Nargus (Demochrus rufipennis (Lucas, 1846, Choleva (Choleva kocheri Henrot, 1962 and Catops fuscus fuscoides Reitter, 1909. The geonemy of these species is updated and the research is illustrated with maps of their distribution.

  16. Effects of pheromone and plant volatile release rates and ratios on trapping Anoplophora glabripennis (Coleoptera: Cerambycidae) in China

    Science.gov (United States)

    P.S. Meng; R.T. Trotter; M.A. Keena; T.C. Baker; S. Yan; E.G. Schwartzberg; K. Hoover

    2014-01-01

    Native to China and Korea, the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae), is a polyphagous wood-boring pest for which a trapping system would greatly benefit eradication and management programs in both the introduced and native ranges. Over two field seasons, a total of 160 flight intercept panel traps...

  17. Intra-annual variation in responses by flying southern pine beetles (Coleoptera: Curculionidae: Scolytinae) to pheromone component endo-brevicomin

    Science.gov (United States)

    Brian T. Sullivan; Cavell Brownie; JoAnne P. Barrett

    2016-01-01

    The southern pine beetle Dendroctonus frontalis Zimmermann (Coleoptera: Curculionidae: Scolytinae) is attracted to an aggregation pheromone that includes the multifunctional pheromone component endobrevicomin. The effect of endo-brevicomin on attractive lures varies from strong enhancement to reduction of beetle attraction depending upon release rate, lure component...

  18. Survival and phenology of Agrilus planipennis (Coleoptera: Buprestidae) reared on a newly developed artificial diet free of host material

    Science.gov (United States)

    Melody A. Keena; Hannah Nadel; Juli. Gould

    2015-01-01

    The final phase in the development of an artificial diet that contains no ash host material and the phenology of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Bupresidae) on that diet are documented. A diet containing powdered ash phloem exists, but host material introduces potential variability and contamination, and the cost and...

  19. Acoustic assessment of Beauveria bassiana (Hypocreales: Clavicipitaceae) effects on Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) larval activity and mortality

    Science.gov (United States)

    Rhynchophorus ferrugineus (Olivier) (Coleoptera: Dryophthoridae), the red palm weevil, is an economically important palm tree pest in subtropical regions of the world. Previous studies have shown that R. ferrugineus can be infected and killed by the entomopathogenic fungus, Beauveria bassiana. Howev...

  20. Sanitation options for managing oak wood infested with the invasive goldspotted oak borer (Coleoptera: Buprestidae) in Southern California

    Science.gov (United States)

    Michael I. Jones; Tom W. Coleman; Andrew D. Graves; Mary Louise. Flint; Steven J. Seybold

    2013-01-01

    Movement of invasive wood-boring insects in wood products presents a threat to forest health and a management challenge for public and private land managers. The goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), is a new pest in San Diego and Riverside Cos., CA, believed to have been introduced on firewood. This beetle...

  1. Limited predation potential by adult and larval lady beetles (Coleoptera: Coccinellidae) on Nezara viridula (Hemiptera: Pentatomidae) eggs and nymphs

    Science.gov (United States)

    The Coccinellidae (Coleoptera) co-occur in many habitats with many arthropods that are of suitable size as prey. The Pentatomidae (Hemiptera) are one such group of insects with eggs and early instars that could be susceptible to predation by Coccinellidae. The objective of this laboratory study wa...

  2. Rearing Xyleborus volvulus (Coleoptera: Curculionidae) on Media Containing Sawdust from Avocado or Silkbay, with or without Raffaelea lauricola (Ophiostomatales: Ophiostomataceae)

    Science.gov (United States)

    Like other ambrosia beetles, Xyleborus volvulus Fabricius (Coleoptera: Curculionidae) lives in a mutualistic symbiotic relationship with fungi that serve as food source. Until recently, X. volvulus was not considered a pest, and none of its symbionts were considered plant pathogens. However, recent ...

  3. Micro-CT unveils the secret life of the coffee berry borer (Hypothenemus hampei; Coleoptera, Curculionidae: Scolytinae) inside coffee berries

    Science.gov (United States)

    The coffee berry borer (Hypothenemus hampei (Ferrari); Coleoptera: Curculionidae: Scolytinae) is the most important insect pest of coffee worldwide, and due to the cryptic life habit of the insect inside coffee berries, effective pest management strategies have been difficult to develop. In this pap...

  4. Spatio-temporal analysis of Xyleborus glabratus (Coleoptera: Circulionidae: Scolytinae) Invasion in Eastern U.S. Forests

    Science.gov (United States)

    F.H. Koch; W.D. Smith

    2008-01-01

    The non-native redbay ambrosia beetle, Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae), has recently emerged as a signiÞcant pest of southeastern U.S. coastal forests. SpeciÞcally, a fungal symbiont (Raffaelea sp.) of X. glabratus has caused mortality of redbay (Persea borbonia...

  5. Effect of temperature on reproduction and embryonic development of the cabbage stem flea beetle, Psylliodes chrysocephala L., (Coleoptera: Chrysomelidae)

    DEFF Research Database (Denmark)

    Mathiasen, Helle; Sørensen, Helle; Bligaard, J.

    2015-01-01

    The cabbage stem flea beetle, Psylliodes chrysocephala (L.) (Coleoptera: Chrysomelidae), is a major pest of winter oilseed rape. Despite the importance of this pest, detailed information on reproduction to predict risk of crop damage is lacking. This study investigates the effect of temperature...

  6. Description and phylogeny of a new microsporidium from the elm leaf beetle, Xanthogaleruca luteola Muller, 1766 (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    This study describes a new genus and species of microsporidia which is a pathogen of the elm leaf beetle, Xanthogaleruca luteola Muller, 1776 (Coleoptera: Chrysomelidae). The beetles were collected from Istanbul in Turkey. All developmental stages are uninucleate and in direct contact with the host ...

  7. The historical role of Ips hauseri (Coleoptera: Curculionidae) in the spruce forest of Ile-Alatausky and Medeo National Parks

    Science.gov (United States)

    N. Mukhamadiev; A. Lynch; C. O' Connor; A. Sagitov; N. Ashikbaev; I. Panyushkina

    2014-01-01

    On 17 May and 27 June 2011 severe cyclonic storms damaged several hundred hectares of spruce forest (Picea schrenkiana) in the Tian Shan Mountains. Bark beetle populations increased rapidly in dead and damaged trees, particularly Ips hauseri, I. typographus, I. sexdentatus, and Piiyogenesperfossus (all Coleoptera: Curculionidae), and there is concern about the...

  8. Can Emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae), emerge from logs two summers after infested trees are cut?

    Science.gov (United States)

    Toby R. Petrice; Robert A. Haack

    2007-01-01

    Emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a serious invasive pest of ash trees (Fraxinus spp.) in North America. Much of EAB's range expansion has been attributed to human-assisted movement of infested items such as ash logs and firewood. It is unclear the amount of time that logs cut...

  9. A review of the natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries.

    Science.gov (United States)

    Di Iorio, Osvaldo

    2014-04-17

    A compilation of the known natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries is provided. Food items of adult Cetoniinae include pollen and/or nectar (flower visitors), sap and/or slime flux, ripened fruits on plants, green tissues and leaves, and honey. Of the 36 species of Cetoniinae from Argentina, food items are known only for 11 species (30.5%). Attraction to light and bait-traps, adult activity periods, vertebrate predators, and the occurrence in bird nests are presented and discussed. Other insects that share the same food sources and bait-traps with Cetoniinae are mentioned.

  10. Nesaeoduvalius (gen. n. vailatii sp. n., from Euboia island (Eastern Greece (Coleoptera: Carabidae, Trechinae

    Directory of Open Access Journals (Sweden)

    Achille Casale

    2016-06-01

    Full Text Available Nesaeoduvalius (new genus vailatii Casale & Giachino, new species (Coleoptera: Carabidae: Trechini is described and illustrated from the Euboia (Evvia island (eastern Greece. The new taxon belongs to the Duvalius “isotopic” trechine lineage, but is markedly characterized by the peculiar combination of several morphological features in the general shape, chaetotaxy of head, serrate lateral margins of elytra, and features of male genitalia (both in median lobe of aedeagus and endophallus. Its putative position amongst the trechines of the Duvalius phyletic lineage is discussed. Data on the distribution and habitat of this new, insular and unexpected taxon are also provided.

  11. Invertebrate fauna (Coleoptera, Collembola, Diplopoda, Isopoda collected in the karst areas of the Aninei - Locvei Mountains

    Directory of Open Access Journals (Sweden)

    Andrei Giurginca

    2011-08-01

    Full Text Available The authors identified 132 species of invertebrates (14 Oniscidea, 25 Diplopoda,31 Collembola and 62 Coleoptera recently sampled (2001–2006 from the soil and subterranean (MSS and caves environments from the Banat Mountains. Some new,rare and endemic species are discussed. The seasonal changes of the species diversity in the superficial subterranean environments at 0.5 to 1 m in depth are for the first time presented for the Reşiţa – Moldova Nouă synclinorium. The characteristic and preferential species for the mesovoid shallow substratum (MSS, belonging to the analyzed taxa, are identified.

  12. Espécies de Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini ocorrentes na Bolívia

    Directory of Open Access Journals (Sweden)

    Maria Helena M. Galileo

    2013-03-01

    Full Text Available Species of Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini occurring in Bolivia. The genus Gorybia (Cerambycinae, Piezocerini consists of 45 described species with seven species recorded from Bolivia. Nine new species are described herein from Bolivia: G. abnormalis sp. nov.; G. alveolata sp. nov.; G. asyka sp. nov.; G. florida sp. nov.; G. inarmata sp. nov.; G. longithorax sp. nov.; G. guenda sp. nov.; G. tuberosa sp. nov. and G. wappesi sp. nov. A key to the species now known to occur in Bolivia is included.

  13. An annotated checklist of the aquatic Polyphaga (Coleoptera) of Egypt I. Family Hydraenidae.

    Science.gov (United States)

    Salah, Mohamed; Cueto, Juan Antonio Régil; Valladares, Luis F

    2014-10-16

    Data from previous literature were used to compile a checklist of the Egyptian fauna of Hydraenidae (Coleoptera). The checklist includes data on the type localities, type specimens, descriptors, distributions and previous literature for 15 valid species belonging to 3 genera (Hydraena, Limnebius and Ochthebius). Ochthebius was represented by 13 species, while Hydraena and Limnebius were represented only by a single species for each of them. The present study provides a summary that can serve as the basis for future progress in the knowledge of the Egyptian Hydraenidae. 

  14. Toxomerus duplicatus Wiedemann, 1830 (Diptera: Syrphidae preying on Microtheca spp. (Coleoptera: Chrysomelidae larvae

    Directory of Open Access Journals (Sweden)

    VS Sturza

    Full Text Available Microtheca spp. (Coleoptera: Chrysomelidae are insect pests primarily related to Brassicaceae crops. In the State of Rio Grande do Sul (RS, southern Brazil, they are found on forage turnip, Raphanus sativus L. var. oleiferus Metzg., which is commonly grown during fall/winter seasons. This work reports the predation of Microtheca spp. larvae by Toxomerus duplicatus Wiedemann, 1830 (Diptera: Syrphidae larvae, on forage turnip crop, in Santa Maria, RS. This register provides new information about Microtheca spp. natural enemies in Brazil, which might be a new option for integrate pest management of these species.

  15. Gross anatomy of central nervous system in firefly, Pteroptyx tener (Coleoptera: Lampyridae)

    Science.gov (United States)

    Hudawiyah, Nur; Wahida, O. Nurul; Norela, S.

    2015-09-01

    This paper describes for the first time the organization and fine structure of the central nervous system (CNS) in the fireflies, Pteroptyx tener (Coleoptera: Lampyridae). The morphology of the CNS was examined by using Carl Zeiss AxioScope A1 photomicroscope with iSolution Lite software. Some specific structural features such as the localization of protocerebrum, deutocerebrum and tritocerebrum in the brain region were analyzed. Other than that, the nerve cord and its peripheral structure were also analyzed. This study suggests that, there is a very obvious difference between male and female central nervous system which illustrates that they may differ in function in controlling physiological and behavioral activities.

  16. Damage of camu-camu (Myrciaria dubia) fruits by Conotrachelus dubiae (Coleoptera: curculionidae) in Central Amazonia

    OpenAIRE

    Ferreira, Sidney Alberto do Nascimento; Gentil, Daniel Felipe de Oliveira; Silva, Neliton Marques da

    2003-01-01

    No Brasil, a ocorrência de Conotrachelus dubiae O'Brien, 1995 (Coleoptera: Curculionidae) em camu-camu [Myrciaria dubia (H.B.K.) McVaugh, Myrtaceae] tinha sido constatada somente em populações naturais. Relata-se sua ocorrência em um cultivo experimental, onde se avaliou os danos de C. dubiae em frutos de camu-camu, em diferentes graus de amadurecimento, entre 1999 e 2003. Os danos causados pela larva aumentaram com o amadurecimento dos frutos, havendo maior comprometimento da polpa do fruto ...

  17. Beneficial effects of a Coriolus versicolor-based vaginal gel on cervical epithelization, vaginal microbiota and vaginal health: a pilot study in asymptomatic women.

    Science.gov (United States)

    Palacios, Santiago; Losa, Fernando; Dexeus, Damián; Cortés, Javier

    2017-03-16

    To assess the effect of a 12-day treatment using a vaginal gel based on niosomes containing hyaluronic acid, ß-glucan, alpha-glucan oligosaccharide, Coriolus versicolor, Asian centella, Azadirachta indica and Aloe vera on vaginal microbiota, cervical epithelization and vaginal health. Open-label, prospective pilot study conducted in asymptomatic women in daily practice. Cervical epithelization was evaluated by colposcopy using an ectopy epithelization score (from 5: no ectopy to 1: severe ectopy and bleeding), vaginal microbiota using the VaginaStatus-Diagnostic test (Instiüt für Mikroökologie, Herborn, Germany) and further rated by the investigator using a 5-point Liker scale (from 5: normal to 1: very severe deterioration in which all evaluated species were altered), and vaginal health using the Vaginal Health Index. In 21 women, a positive effect to improve epithelization of the cervical mucosa, with a mean score of 4.42 at the final visit as compared to 3.09 at baseline (P vaginal microbiota status, with a mean score of 4.0 at the final visit vs. 3.3 at baseline (P = NS) (21.2% improvement). In 11 women, the Vaginal Health Index increased from 19.0 at baseline to 22.3 at the final visit (P = 0.007). The concentration of Lactobacillus spp. increased 54.5% of women and pH decreased from 4.32 to 4.09. These encouraging preliminary results provide the basis for designing a randomized controlled study, and for potential use in human papilloma virus infection. ISRCTN77955077 . Registration date: February 15, 2017. Retrospectively registered.

  18. Soil and saproxylic species (Coleoptera, Collembola, Araneae in primeval forests from the Northern part of South-Easthern Carpathians

    Directory of Open Access Journals (Sweden)

    Eugen Nițu

    2009-12-01

    Full Text Available In 2006-2007 we carried out faunal investigations in the vernal, estival and autumnal seasons in the scientific reserve "Codrul Secular Giumalău" using quantitative sampling methods. We identified 189 species of Coleoptera, 70 of Collembola and 20 of Araneae. Of these, 11 phytophagous, 18 myceto/xylo-mycetophagous, 9 mixophagous, 18 xylo- and cambio-xylemophagous, 38 saproxylophagous, 125 (55 Coleoptera, 70 Collembola detritivorous (sapro-, copro- and necrophagous, 60 (40 Coleoptera, 20 Aranea predators/parasitoids. Hymenaphorura polonica Pomorski, 1990 (Collembola, and Leiodes rhaeticus Erichson, 1845 (Coleoptera, Leiodidae, are recorded for the first time in the Romanian fauna. The rare species and characteristic species for the old primeval spruce forests are analysed for each studied taxonomic group. The species richness and faunal diversity from the Giumalău primeval spruce forest are compared with those of other very well preserved forests from the Carpathians scientific reserves (Codrul Secular Slătioara, Pietrosul Rodnei. The species abundances were used to compute the similarity indexes between the sampled sectors of forest and to perform Cluster Analysis. We observed that the dead wood in the 2nd-6th phases of decomposition has a great influence not only on the saproxylic species but also on the soil fauna like ground beetles (Carabidae that use the logs as ecologic microrefuges (winter refugees or diurnal refugees. The structure of the soil fauna is influenced by wood extraction from the forest ecosystem or by natural perturbations, this consisting in the appearance of opportunistic species as Orchesella pontica (Collembola and in decreasing of species richness of Carabidae (Coleoptera.

  19. Soil and saproxylic species (Coleoptera, Collembola, Araneae in primeval forests from the northern part of South-Easthern Carpathians

    Directory of Open Access Journals (Sweden)

    Eugen Nitu

    2009-11-01

    Full Text Available In 2006-2007 we carried out faunal investigations in the vernal, estival and autumnal seasons in the scientific reserve "Codrul Secular Giumalãu" using quantitative sampling methods. We identified 189 species of Coleoptera, 70 of Collembola and 20 of Araneae. Of these, 11 phytophagous, 18 myceto/xylo-mycetophagous,9 mixophagous, 18 xylo- and cambio-xylemophagous, 38 saproxylophagous,125 (55 Coleoptera, 70 Collembola detritivorous (sapro-, copro- andnecrophagous, 60 (40 Coleoptera, 20 Aranea predators/parasitoids. Hymenaphorura polonica Pomorski, 1990 (Collembola, and Leiodes rhaeticus Erichson, 1845 (Coleoptera, Leiodidae, are recorded for the first time in the Romanian fauna. The rare species and characteristic species for the old primeval spruce forests are analysed for each studied taxonomic group. The species richness and faunal diversity from the Giumalãu primeval spruce forest are compared with those of other very well preserved forests from the Carpathians scientific reserves (Codrul Secular Slãtioara,Pietrosul Rodnei. The species abundances were used to compute the similarity indexes between the sampled sectors of forest and to perform Cluster Analysis. We observed that the dead wood in the 2nd-6th phases of decomposition has a great influence not only on the saproxylic species but also on the soil fauna like ground beetles(Carabidae that use the logs as ecologic microrefuges (winter refugees or diurnal refugees. The structure of the soil fauna is influenced by wood extraction from the forest ecosystem or by natural perturbations, this consisting in the appearance of opportunistic species as Orchesella pontica (Collembola and in decreasing ofspecies richness of Carabidae (Coleoptera.

  20. Functional Response of the Predators Alloeocranum biannulipes (Hemiptera: Reduviidae) and Teretrius nigrescens (Coleoptera: Histeridae) Feeding on Dinoderus porcellus (Coleoptera: Bostrichidae) Infesting Yam Chips.

    Science.gov (United States)

    Loko, Y L; Djagoun, A D; Dannon, E A; Datinon, B; Dansi, A; Thomas-Odjo, A A; Tamo, M

    2017-02-01

    The functional response and some predation parameters of the predators Alloeocranum biannulipes Montrouzier & Signoret (Hemiptera: Reduviidea) and Teretrius nigrescens Lewis (Coleoptera: Histeridae) were evaluated at five different densities of larvae and pupae of Dinoderus porcellus Lesne (Coleoptera: Bostrichidae) with the aim to understand their roles in the biological control of this major pest of stored yam chips. Experiments were performed in petri dishes at 25 ± 1 °C, 60 ± 10% RH, and a photoperiod of 12:12 (L:D) h in a controlled temperature room. Both predators showed Type II of functional response with respect to larvae, determined by a logistic regression model. However, T. nigrescens significantly killed more larvae of D. porcellus compared with A. biannulipes. This behavior, however, changed to a linear functional response (Type I), when pupae of D. porcellus were offered to both predators, possibly because of their immobility. In addition, there was no significant difference between T. nigrescens and A. biannulipes in terms of the killed pupae. Parameters of the Holling disc equation for both predators were estimated. Estimated handling time on larvae of D. porcellus for T. nigrescens and A. biannulipes was 0.254 and 0.677 h and the rate of searching efficiency was 0.289 and 0.348 h-1, respectively. Results indicated that T. nigrescens was a more suitable candidate for augmentative release for D. porcellus control than A. biannulipes. However, semifield studies are required to draw firm conclusions. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  1. Estirpes de Bacillus thuringiensis efetivas contra insetos das ordens Lepidoptera, Coleoptera e Diptera Bacillus thuringiensis strains effective against insects of Lepidoptera, Coleoptera and Diptera orders

    Directory of Open Access Journals (Sweden)

    Lílian Botelho Praça

    2004-01-01

    Full Text Available O objetivo deste trabalho foi selecionar entre 300 estirpes de Bacillus thuringiensis as efetivas simultaneamente contra larvas de Spodoptera frugiperda J.E. Smith e Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae, Anthonomus grandis Boheman (Coleoptera: Curculionidae, Aedes aegypti Linnaeus e Culex quinquefasciatus Say (Diptera: Culicidae. Foram selecionadas duas estirpes de B. thuringiensis, denominadas S234 e S997, que apresentaram atividade contra as três ordens de insetos. As estirpes foram caracterizadas por métodos morfológicos, bioquímicos e moleculares. As mesmas apresentaram duas proteínas principais de 130 e 65 kDa, produtos de reação em cadeia da polimerase de tamanho esperado para a detecção dos genes cry1Aa, cry1Ab, cry1Ac, cry1B e cry2 e cristais bipiramidais, cubóides e esféricos.The aim of this work was to select among 300 strains of Bacillus thuringiensis those which are simultaneously effective against larvae of Spodoptera frugiperda J.E. Smith and Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae, Anthonomus grandis Boheman (Coleoptera: Curculionidae, Aedes aegypti Linnaeus and Culex quinquefasciatus Say (Diptera: Culicidae. Two strains of B. thuringiensis were selected, S234 and S997, which presented activity against those three insect orders. Both strains were characterized by morphological, biochemical and molecular methods. They have presented two main proteins with 130 and 65 kDa, polimerase chain reaction products with expected sizes for detection of the genes cry1Aa, cry1Ab, cry1Ac, cry1B and cry2 and bipiramidal, cubical and spherical crystals.

  2. Biology of Pityophthorus pulchellus tuberculatus (Coleoptera: Curculionidae: Scolytinae) in Lodgepole Pine in Northern Idaho.

    Science.gov (United States)

    Furniss, Malcolm M; Kegley, Sandra J

    2018-04-19

    The twig beetle, Pityophthorus pulchellus tuberculatus Eichhoff, infests dead branches of pines in western United States and Canada, including lodgepole pine, Pinus contorta Douglas, in northern Idaho. Adult broods overwintered in their host and emerged and colonized new hosts in late April. Males initiated galleries and were joined by up to seven females, each of which constructed an egg gallery radiating from a central chamber. Galleries had an average of 4.7 egg niches each with an egg that was large relative to the mother beetle. Two larval instars were recognized. Dentition of larval mandibles differed in shape from that in literature. Mature larvae pupated either in a cell excavated on the wood surface or in a cell below the wood surface. First-generation adults mined extensively in the inner bark and wood before emerging to infest new trees in late June. Their progeny became adults beginning in early August and likewise mined and fed on the inner bark and wood before overwintering. Predacious beetles present as larvae in the galleries included Enoclerus lecontei (Wolcott) (Coleoptera: Cleridae) and Lasconotus sp. (Coleoptera: Zopheridae). Parasitoid Hymenoptera reared from infested trees were Cosmophorus pityophthori Rohwer (Braconidae), Phasmidiasta n. sp. (Braconidae), Spathius sp. (Braconidae), Acerocephala n. sp. (Pteromalidae), Metacolus fasciatus Girault (Pteromalidae), Rhaphitelus maculatus Walker (Pteromalidae), Rhopalicus sp. (Pteromalidae), and an unidentified pteromalid.

  3. Adaptation of the Egg of the Desert Beetle, Microdera punctipennis (Coleoptera: Tenebrionidae), to Arid Environment

    Science.gov (United States)

    Wang, Yan; Shi, Meng; Hou, Xiaojuan; Meng, Shanshan; Zhang, Fuchun; Ma, Ji

    2014-01-01

    Abstract Microdera punctipennis Kaszab (Coleoptera: Tenebrionidae) is an endemic species in Guerbantonggut desert in China. To explore the ways that M. punctipennis egg adapts to dry desert environment, morphological characteristics of the egg was investigated along with the egg of the nondesert beetle Tenebrio molitor (Coleoptera: Tenebrionidae). Water loss rate and survival rate of these eggs under different dry treatments (relative humidity0, 10, and 20%) were measured to evaluate the desiccation resistance of the eggs at different developmental stages (day 0, 2, and 5 eggs). Our results showed that the 50-egg weight in T. molitor was heavier than M. punctipennis , while the 50-first-instar larva weight in T. molitor was almost the same as in M. punctipennis . The water loss rate of M. punctipennis egg under dry conditions was significantly lower than T. molitor , and the egg survival rate was significantly higher than T. molitor . The estimated developmental threshold temperature of M. punctipennis egg was 18.30°C, and the critical thermal maximum of M. punctipennis egg is above 39°C. These features partly account for the adaptability of M. punctipennis to desert environment in egg stage. PMID:25525108

  4. The hydraulic mechanism in the hind wing veins of Cybister japonicus Sharp (order: Coleoptera

    Directory of Open Access Journals (Sweden)

    Jiyu Sun

    2016-06-01

    Full Text Available The diving beetles (Dytiscidae, Coleoptera are families of water beetles. When they see light, they fly to the light source directly from the water. Their hind wings are thin and fragile under the protection of their elytra (forewings. When the beetle is at rest the hind wings are folded over the abdomen of the beetle and when in flight they unfold to provide the necessary aerodynamic forces. In this paper, the unfolding process of the hind wing of Cybister japonicus Sharp (order: Coleoptera was investigated. The motion characteristics of the blood in the veins of the structure system show that the veins have microfluidic control over the hydraulic mechanism of the unfolding process. A model is established, and the hind wing extending process is simulated. The blood flow and pressure changes are discussed. The driving mechanism for hydraulic control of the folding and unfolding actions of beetle hind wings is put forward. This can assist the design of new deployable micro air vehicles and bioinspired deployable systems.

  5. Habitat preferences of ground beetle (Coleoptera: Carabidae) species in the northern Black Hills of South Dakota.

    Science.gov (United States)

    Bergmann, David J; Brandenburg, Dylan; Petit, Samantha; Gabel, Mark

    2012-10-01

    Ground beetles (Coleoptera: Carabidae) are a major component of terrestrial invertebrate communities and have been used as bioindicators of habitat change and disturbance. The Black Hills of South Dakota is a small area with a high biodiversity, but the ground beetles of this region are little studied. The habitat preferences of ground beetles in the Black Hills are unknown, and baseline data must be collected if these beetles are to be used in the future as bioindicators. Ground beetles (Coleoptera: Carabidae) were collected from pitfall traps at two sites in each of five kinds of habitats (grassland, bur oak-ironwood forests, ponderosa pine-common juniper forests, aspen-pine forests, and a spruce forest) from which habitat structure characteristics and plant abundance data also were collected. In total, 27 species of ground beetles were identified. Although some species, such as Dicaelus sculptilis Say were found in most habitats, other species showed distinct habitat preferences: Poecilus lucublandus (Say) preferred oak forests, Pasimachus elongatus LeConte preferred grasslands, and Calathus ingratus Dejean preferred high-elevation aspen-pine forests. Pterostichus adstrictus Escholtz was found only in woodlands, and Carabus taedatus Say strictly in higher elevation (over 1,500 m) aspen or coniferous woods, and may represent relict populations of boreal species. Elevation, exposure to sunlight, and cover of woody plants strongly influence the structure of carabid communities in the Black Hills.

  6. Detection and quantification of Leptographium wageneri, the cause of black-stain root disease, from bark beetles (Coleoptera: Scolytidae) in North California using regular and real-time PCR

    Science.gov (United States)

    Wolfgang Schweigkofler; William J. Otrosina; Sheri L. Smith; Daniel R. Cluck; Kevin Maeda; Kabir G. Peay; Matteo Garbelotto

    2005-01-01

    Black-stain root disease is a threat to conifer forests in western North America. The disease is caused by the ophiostomatoid fungus Leptographium wageneri (W.B. Kendr.) M.J. Wingf., which is associated with a number of bark beetle (Coleoptera: Scolytidae) and weevil species (Coleoptera: Curculionidae). We developed a polymerase chain reaction test...

  7. Solid-state fermentation of Jatropha seed cake for optimization of lipase, protease and detoxification of anti-nutrients in Jatropha seed cake using Aspergillus versicolor CJS-98.

    Science.gov (United States)

    Veerabhadrappa, Mohankumar Bavimane; Shivakumar, Sharath Belame; Devappa, Somashekar

    2014-02-01

    This study focused on the solid-state fermentation of Jatropha seed cake (JSC), a byproduct generated after biodiesel production. Presence of anti-nutritional compounds and toxins restricts its application in livestock feed. The disposal of the JSC is a major environmental problem in the future, due to the generation of huge quantity of JSC after biodiesel extraction. Hence the JSC was assessed for its suitability as substrate for production and optimization of lipase and protease from Aspergillus versicolor CJS-98 by solid-state fermentation (SSF). The present study was also focused on the biodetoxification of anti-nutrients and toxins in JSC. The SSF parameters were optimized for maximum production of lipase and protease. Under the optimized conditions, the JSC supplemented with maltose and peptone (2%), adjusted to pH 7.0, moisture content 40%, inoculated with 1 × 10(7) spores per 5 g cake and incubated at 25°C, produced maximum lipase, 1288 U/g and protease, 3366 U/g at 96 h. The anti-nutrients like phytic acid (6.08%), tannins (0.37%), trypsin inhibitors (697.5 TIU/g), cyanogenic glucosides (692.5 μg/100 g), and lectins (0.309 mg/ml), were reduced to 1.70%, 0.23%, 12.5 TIU/g, 560.6 μg/100 g and 0.034 mg/ml respectively. The main toxic compound phorbol esters content in the JSC was reduced from 0.083% to 0.015% after SSF. Our results indicate that viability of SSF to utilize the huge amount of seed cake generated after extraction of biodiesel, for production of industrial enzymes and biodetoxification of anti-nutrients, toxins. Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  8. ESCARABAJOS TIGRE (COLEOPTERA: CICINDELIDAE DEL MUSEO ENTOMOLÓGICO FRANCISCO LUÍS GALLEGO: NUEVOS REGISTROS PARA DEPARTAMENTOS DE COLOMBIA TIGER BEETLES (COLEOPTERA: CICINDELIDAE AT THE ENTOMOLOGICAL MUSEUM FRANCISCO LUÍS GALLEGO: NEW RECORDS

    Directory of Open Access Journals (Sweden)

    Manuel Alejandro Ramírez Mora

    2008-06-01

    Full Text Available Se registran por primera vez las especies de escarabajos tigre (Coleoptera: Cicindelidae presentes en el Museo Entomológico Francisco Luis Gallego (MEFLG. Se identificaron 167 especimenes distribuidos en ocho géneros y 27 especies, se reportan 24 nuevos registros para diferentes departamentos de Colombia. Se señalan aspectos importantes de la taxonomía y sistemática del grupo. Además, se presentan comentarios biológicos y de distribución de las especies.Tiger beetles (Coleoptera: Cicindelidae species at the Museo Entomológico Francisco Luis Gallego (MEFLG are registered by first time. 167 specimens in 8 genus and 27 species were identified, 24 new records for different Colombian states are reported. Some important aspects of the group’s taxonomy and systematic are pointed. Additionally, species’ biological and distributional comments are presented.

  9. Peculiarities of the imago Coleoptera (Insecta groups overwintering in various substrata of the Reserve «Galichya Gora»

    Directory of Open Access Journals (Sweden)

    Mikhail N. Tsurikov

    2016-05-01

    Full Text Available During the 1997–2006 and 2010–2011winter seasons, in the area of Morozova Gora (the nature reserve "Galichya Gora", in Russia, 1200 samples of various substrata were taken, most of which were 4500 cm3 in volume. In total, 41,854 specimens of 690 species belonging to 52 Coleoptera families were registered at overwintering sites. The analysis of the peculiarities of imago Coleoptera groups in the major winter habitats showed that in most of the investigated substrata representatives of the Staphylinidae family prevailed both in terms of species diversity and number. It is only under the bark of trees and in deadwood that Carabidae are the most numerous, whereas Latridiidae are prevalent in tinder fungi. Turf has the maximal species saturation during the winter season (the highest percentage of species referring to 18 families was registered here, as well as plant litter (10 families, with turf being the preference of 8 families richest in species diversity. The imagos of a number of families relatively rich in species – Cantharidae, Malachiidae, Melandryidae, Mordellidae, Oedemeridae, Meloidae, Scraptiidae and Anthribidae – were not found at overwintering sites, which is explained by the preimaginal overwintering of most representatives of the abovementioned families. It was shown that in substrata which are the least suitable for the overwintering of the imago of most Coleoptera species, the highest percentage of the predominant species was registered since more accessible substrata are used as overwintering sites by the same species from different habitats, which decreases the concentration of imago beetles of certain species there. A study of the peculiarities of species distribution (with no less than 30 specimens among overwintering sites showed that the largest number of stenotopic species was registered in droppings (9 species. Then follow the substrata (in decreasing order: turf (5, hay (grass sward, haymow, meadow (4, decomposing

  10. Fauna de coleópteros aquáticos (insect: coleoptera na Amazônia central, Brasil Aquatic Beetlefauna (insecta: coleoptera in Central Amazonia, Brazil

    Directory of Open Access Journals (Sweden)

    Cesar João Benetti

    2003-12-01

    Full Text Available Foram coletados 888 exemplares de Coleoptera aquáticos nos municípios de Manaus, Manacapuru e Presidente Figueiredo (AM, de fevereiro de 2000 a agosto de 2002, distribuídos em 12 famílias, 50 gêneros e 88 espécies ou morfoespécies. Novas ocorrências para o Brasil, incluem as seguintes espécies de Dytiscidae: Hydrodessus robinae, H. surinamensis, Hypodessus frustrator, Neobidessus confusus, N. spangleri e N. woodruffi. Os gêneros Agaporomorphus (Dytiscidae e Pronoterus (Noteridae são registrados pela primeira vez para o estado do Amazonas, assim como as espécies: P. punctipennis e Suphisellus nigrinus (Noteridae; Agaporomorphus grandisinuatus, Bidessonotus tibialis, Derovatellus lentus,Desmopachria nitida, Hydaticus xanthomelas, Laccophilus tarsalis, Liodessus affinis e Megadytes laevigatus (Dytiscidae. A família Dytiscidae foi a que apresentou maior riqueza, com 34 espécies, seguida de Hydrophilidae, com 20 e Noteridae, com 12 espécies. Os gêneros com maior número de espécies foram Gyretes (Gyrinidae e Suphisellus (Noteridae com 6 espécies, Copelatus (Dytiscidae e Tropisternus (Hydrophilidae, com 5 espécies.In this work, 888 specimens of aquatic Coleoptera were collected in Manaus, Manacapuru and Presidente Figueiredo counties (AM, distributed in 12 families, 50 genera and 88 species or morphospecies. New occurrences in Brasil include the following species of Dytiscidae: Hydrodessus robinae, H. surinamensis, Hypodessus frustrator, Neobidessus confusus, N. spangleri and N. woodruffi. The genera Agaporomorphus (Dytiscidae and Pronoterus (Noteridae were reported for the first time in the State of Amazonas, as well as the species P. punctipennis and Suphisellus nigrinus (Noteridae; Agaporomorphus grandisinuatus, Bidessonotus tibialis, Derovatellus lentus,Desmopachria nitida, Hydaticus xanthomelas, Laccophilus tarsalis, Liodessus affinis and Megadytes laevigatus (Dytiscidae. The family Dytiscidae presented the highest richness

  11. Boundaries in ground beetle (Coleoptera: Carabidae) and environmental variables at the edges of forest patches with residential developments

    OpenAIRE

    Davis, Doreen E.; Gagné, Sara A.

    2018-01-01

    Background Few studies of edge effects on wildlife objectively identify habitat edges or explore non-linear responses. In this paper, we build on ground beetle (Coleoptera: Carabidae) research that has begun to address these domains by using triangulation wombling to identify boundaries in beetle community structure and composition at the edges of forest patches with residential developments. We hypothesized that edges are characterized by boundaries in environmental variables that correspond...

  12. Especies de dryophthorinae (coleoptera: curculionidae) asociadas a plátano y banano (musa spp.) en colombia

    OpenAIRE

    RUBIO-GOMEZ, JOSE; SEPULVEDA-CANO, PAULA

    2009-01-01

    RESUMEN

    Se presenta una sinopsis de los escarabajos de la subfamilia Dryophthorinae (Coleoptera: Curculionidae) asociados a cultivos de plátano y banano en Colombia. Adicionalmente se ofrecen claves ilustradas para las especies del país. Se registran seis especies asociadas a dichos cultivos: Cosmopolites sordidus (Germar, 1824), Metamasius hemipterus (Linnaeus, 1758), Metamasius hebetatus (Gyllenhal, 1838), Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum...

  13. A new species of Bruchidius (Coleoptera: Chrysomelidae: Bruchinae) from Albizia in Northern Thailand and a review of Bruchidius group 5.

    Science.gov (United States)

    Tuda, Midori

    2008-04-01

    A new species, Bruchidius paicus (Insecta, Coleoptera) reared from the seeds of a leguminous tree, Albizia lebbeck (Fabaceae: Mimosoideae: Ingeae), is described from Northern Thailand. Inspection of genital and external morphological traits of B. paicus revealed that the new species belongs to Bruchidius Group 5 (sensu ). The definition of Group 5 is reviewed based on both external and genital morphology. Further comparison of the group to molecular Clade I of Bruchidius (sensu ) indicates the two groups correspond to each other.

  14. The response of ground beetles (Coleoptera: Carabidae) to selection cutting in a South Carolina bottomland hardwood forest

    Science.gov (United States)

    Michael D. Ulyshen; James L. Hanula; Scott Horn; John C. Kilgo; Christopher E. Moorman

    2005-01-01

    We compared the response of ground beetles (Coleoptera: Carabidae) to the creation of canopy gaps of different size (0.13, 0.26, and 0.50 ha) and age (1 and 7 years) in a bottomland hardwood forest (South Carolina, USA). Samples were collected four times in 2001 by malaise and pitfall traps placed at the center and edge of each gap, and 50 m into the surrounding forest...

  15. Alphus marinonii sp. nov., nova espécie para o Peru e Brasil (Coleoptera, Cerambycidae, Lamiinae

    Directory of Open Access Journals (Sweden)

    Diego de Santana Souza

    2013-03-01

    Full Text Available Alphus marinonii sp. nov., new species from Peru and Brazil (Coleoptera, Cerambycidae, Lamiinae. A new species of Alphus, A. marinonii sp. nov., from Peru and Brazil (Rondônia is described. Key to identification and pictures for the four species of the genus are provided. Notes on distribution of A. tuberosus are included, with a new record for Peru and Brazil (Goiás and Mato Grosso do Sul.

  16. A new brachypterous scarab species, Orphnus longicornis (Coleoptera: Scarabaeidae: Orphninae), from the East African Rift.

    Science.gov (United States)

    Frolov, Andrey; Akhmetova, Lilia

    2015-11-05

    The Afrotropical Region is the center of the diversity of the scarab beetle genus Orphnus MacLeay, 1819 (Coleoptera: Scarabaeidae: Orphninae), with 94 species occurring from Sahel in the north to Little Karoo in the south (Paulian, 1948; Petrovitz, 1971; Frolov, 2008). The East African Rift is one of the richest regions of the Afrotropics housing more than 20 species of Orphnus (Paulian, 1948; Frolov, 2013), most of which are endemic to this region. Yet the scarab beetle fauna of the East African Rift, and especially the Eastern Arc Mountains, is still inadequately studied. Examination of the material housed in the Museum of Natural History of Humboldt-Universität, Berlin, Germany (ZMHUB), revealed a series of brachypterous Orphnus beetles belonging to an undescribed species. The new species is described and illustrated below.

  17. Identification of candidate chemosensory genes in the antennal transcriptome of Tenebrio molitor (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Liu, Su; Rao, Xiang-Jun; Li, Mao-Ye; Feng, Ming-Feng; He, Meng-Zhu; Li, Shi-Guang

    2015-03-01

    We present the first antennal transcriptome sequencing information for the yellow mealworm beetle, Tenebrio molitor (Coleoptera: Tenebrionidae). Analysis of the transcriptome dataset obtained 52,216,616 clean reads, from which 35,363 unigenes were assembled. Of these, 18,820 unigenes showed significant similarity (E-value molitor OBPs and CSPs are closely related to those of T. castaneum. Real-time quantitative PCR assays showed that eight TmolOBP genes were antennae-specific. Of these, TmolOBP5, TmolOBP7 and TmolOBP16 were found to be predominantly expressed in male antennae, while TmolOBP17 was expressed mainly in the legs of males. Several other genes were identified that were neither tissue-specific nor sex-specific. These results establish a firm foundation for future studies of the chemosensory genes in T. molitor. Copyright © 2015 Elsevier Inc. All rights reserved.

  18. Cellulose digestion in Monochamus marmorator Kby. (coleoptera: Cerambycidae): role of acquired fungal enzymes

    International Nuclear Information System (INIS)

    Kukol, J.J.; Martin, M.M.

    1986-01-01

    Larvae of the balsam fir sawyer, Monochamus marmorator Kby. (Coleoptera, Cerambycidae), contain midgut digestive enzymes active against hemicellulose and cellulose. Cellulases from larvae fed on balsam fir wood infected with the fungus, Trichoderma harzianum Rifai (Deuteromycetes, Moniliales, Moniliaceae), were found to be identical to those of the cellulase complex produced by this fungus when compared using chromatography, electrophoresis, and isofocusing. When larvae are maintained on a fungusfree diet, their midgut fluids lack cellulolytic activity, and they are unable to digest cellulose. Cellulolytic capacity can be restored by feeding the larvae wood permeated by fungi. We conclude that the enzymes which enable M. marmorator larvae to digest cellulose are not produced by the larvae. Instead, the larvae acquire the capacity to digest cellulose by ingesting active fungal cellulases while feeding in fungus-infected wood

  19. Cellulose digestion in Monochamus marmorator Kby. (coleoptera: Cerambycidae): role of acquired fungal enzymes

    Energy Technology Data Exchange (ETDEWEB)

    Kukol, J.J.; Martin, M.M.

    1986-05-01

    Larvae of the balsam fir sawyer, Monochamus marmorator Kby. (Coleoptera, Cerambycidae), contain midgut digestive enzymes active against hemicellulose and cellulose. Cellulases from larvae fed on balsam fir wood infected with the fungus, Trichoderma harzianum Rifai (Deuteromycetes, Moniliales, Moniliaceae), were found to be identical to those of the cellulase complex produced by this fungus when compared using chromatography, electrophoresis, and isofocusing. When larvae are maintained on a fungusfree diet, their midgut fluids lack cellulolytic activity, and they are unable to digest cellulose. Cellulolytic capacity can be restored by feeding the larvae wood permeated by fungi. We conclude that the enzymes which enable M. marmorator larvae to digest cellulose are not produced by the larvae. Instead, the larvae acquire the capacity to digest cellulose by ingesting active fungal cellulases while feeding in fungus-infected wood.

  20. The new record for Turkish invasive alien insect fauna Anoplophora chinensis (Forster, 1771 (Coleoptera: Cerambycidae

    Directory of Open Access Journals (Sweden)

    Erdem Hızal

    2015-01-01

    Full Text Available International trade is increasing rapidly with developing transportation routes. As a result of this, it became easier for many animal species to move from their natural habitats with the break down of the natural barriers between countries and continents. Insects take an important place among these animals. Insect populations are controlled by several factors in their natural habitats, but they causes important problems as they move by living plants and wood materials to another area. They are so called invasive alien insect species in their new location. These species’ common characteristics are fast growth and reproduction, high dispersal ability, tolerance of wide range of enviromental conditions and ability to feed with various food types. The increase in importing of the plants and wood material in the recent years has been causing the prensence of these species in Turkey. In this research Anoplophora chinensis (Forster, 1771 (Coleoptera; Cerambycidae is given as a new record to Turkish invasive alien insect species fauna.

  1. Feeding habits of Carabidae (Coleoptera associated with herbaceous plants and the phenology of coloured cotton

    Directory of Open Access Journals (Sweden)

    Danilo Henrique da Matta

    2017-04-01

    Full Text Available The carabids (Coleoptera: Carabidae are recognized as polyphagous predators and important natural enemies of insect pests. However, little is known about the feeding habits of these beetles. In this work, we determine the types of food content in the digestive tracts of nine species of Carabidae associated with herbaceous plants and different growth stages of coloured cotton. The food contents were evaluated for beetles associated with the coloured cotton cv. BRS verde, Gossypium hirsutum L. latifolium Hutch., adjacent to weed plants and the flowering herbaceous plants (FHPs Lobularia maritima (L., Tagetes erecta L., and Fagopyrum esculentum Moench. The digestive tract analysis indicated various types of diets and related arthropods for Abaris basistriata, Galerita brasiliensis, Scarites sp., Selenophorus alternans, Selenophorus discopunctatus and Tetracha brasiliensis. The carabids were considered to be polyphagous predators, feeding on different types of prey.

  2. New records of water beetles (Coleoptera: Haliplidae, Dytiscidae, Gyrinidae from Montenegro (SE Europe

    Directory of Open Access Journals (Sweden)

    Pešić Vladimir M.

    2005-01-01

    Full Text Available The water beetle fauna of Montenegro is still poorly known. In the catalog dealing with water beetles (Hydrochantares and Palpicornia in Yugoslavia Gueorguiev (1971 gives a list of 116 water beetle species from Montenegro. Mikšić (1977 reported the presence of six water beetles species from the Ulcinj area. In the present paper, 19 water beetle species (Coleoptera Hydradephaga are reported, five of which are new for the fauna of Montenegro. All specimens have been deposited in the zoological collection of the department of Biology (Podgorica. In list of the species, we give the locality, the date of sampling, the total number of individuals and the names of collectors.

  3. What do we know about winter active ground beetles (Coleoptera, Carabidae in Central and Northern Europe?

    Directory of Open Access Journals (Sweden)

    Radomir Jaskula

    2011-05-01

    Full Text Available This paper summarizes the current knowledge on winter active Carabidae in Central and Northern Europe. In total 73 winter active species are listed, based on literature and own observations. Ground beetles are among the three most numerous Coleoptera families active during the autumn to spring period. The winter community of Carabidae is composed both of larvae (mainly autumn breeding species and adults, as well as of epigeic species and those inhabiting tree trunks. Supranivean fauna is characterized by lower species diversity than the subnivean fauna. The activity of ground beetles decreases in late autumn, is lowest during mid-winter and increases in early spring. Carabidae are noted as an important food source in the diet of insectivorous mammals. They are also predators, hunting small winter active invertebrates.

  4. What do we know about winter active ground beetles (Coleoptera, Carabidae) in Central and Northern Europe?

    Science.gov (United States)

    Jaskuła, Radomir; Soszyńska-Maj, Agnieszka

    2011-01-01

    This paper summarizes the current knowledge on winter active Carabidae in Central and Northern Europe. In total 73 winter active species are listed, based on literature and own observations. Ground beetles are among the three most numerous Coleoptera families active during the autumn to spring period. The winter community of Carabidae is composed both of larvae (mainly autumn breeding species) and adults, as well as of epigeic species and those inhabiting tree trunks. Supranivean fauna is characterized by lower species diversity than the subnivean fauna. The activity of ground beetles decreases in late autumn, is lowest during mid-winter and increases in early spring. Carabidae are noted as an important food source in the diet of insectivorous mammals. They are also predators, hunting small winter active invertebrates.

  5. Incorporating a Sorghum Habitat for Enhancing Lady Beetles (Coleoptera: Coccinellidae in Cotton

    Directory of Open Access Journals (Sweden)

    P. G. Tillman

    2012-01-01

    Full Text Available Lady beetles (Coleoptera: Coccinellidae prey on insect pests in cotton. The objective of this 2 yr on-farm study was to document the impact of a grain sorghum trap crop on the density of Coccinellidae on nearby cotton. Scymnus spp., Coccinella septempunctata (L., Hippodamia convergens Guérin-Méneville, Harmonia axyridis (Pallas, Coleomegilla maculata (De Geer, Cycloneda munda (Say, and Olla v-nigrum (Mulsant were found in sorghum over both years. Lady beetle compositions in sorghum and cotton and in yellow pyramidal traps were similar. For both years, density of lady beetles generally was higher on cotton with sorghum than on control cotton. Our results indicate that sorghum was a source of lady beetles in cotton, and thus incorporation of a sorghum habitat in farmscapes with cotton has great potential to enhance biocontrol of insect pests in cotton.

  6. The mortality of Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae induced by powdered plants

    Directory of Open Access Journals (Sweden)

    Kłyś Małgorzata

    2015-01-01

    Full Text Available The aim of this study was to investigate whether powdered plants of different species namely: peppermint Mentha piperita (L. (Lamiaceae, wormwood Artemisia absinthium (L. (Asteraceae, common sage Salvia officinalis (L. (Lamiaceae, allspice Pimenta dioica (Linnaeus et Merrill (Myrtaceae and common garlic Allium sativum (L. (Amaryllidaceae, added to semolina using concentrations of 1.23, 3.61, and 5.88%, influence the mortality rate in the saw-toothed grain beetle Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae. Experiments were conducted in a laboratory at 28°C and relative humidity 60±5%. At the concentration of 1.23%, allspice seeds caused the highest mortality amongst the saw-toothed grain beetle. When concentrations of 3.61 and 5.88% were used, sage, peppermint and wormwood caused the highest statistically significant mortality of O. surinamensis

  7. The tiger beetles (Coleoptera: Carabidae, Cicindelinae) of Angola: a descriptive catalogue and designation of neotypes.

    Science.gov (United States)

    Serrano, Artur R M; Capela, Rúben A

    2013-11-01

    An annotated catalogue of the species and subspecies of tiger beetles (Coleoptera: Carabidae, Cicindelinae) hitherto known from Angola is given. A total of 89 forms (74 species and 15 subspecies) is recorded from this southwestern country of Africa. Within this assemblage there are 31 endemic forms (33.3%). Some species are represented by only the holotype specimen (some without locality) or the type series. Others were recorded based on a single specimen. Records for six species previously unknow from Angola are given: Foveodromica sp. n. 1, Foveodromica sp. n. 2, Ophryodera rufomarginata bradshawi Péringuey, 1888, Elliptica muata parallelestriata (W. Horn, 1923), Lophyra differens (W. Horn, 1892) and Myriochila jucunda (Péringuey, 1892). A historical review, as well as some considerations on the distribution and conservation status of these beetles in Angola are also presented.

  8. NEW CONTRIBUTION CONCERNING THE MASS REARING OF TANYMECUS DILATICOLLIS GYLL (COLEOPTERA: CURCULIONIDAE UNDER CONTROLLED CONDITIONS

    Directory of Open Access Journals (Sweden)

    Traian MANOLE

    2013-12-01

    Full Text Available Controlled growth in the laboratory of some species of insect’s are justified both scientifically and practically. Due to the economic importance that the corn crop pest Tanymecus dilaticollis Gyll. (Coleoptera: Curculionidae had in Romania like the main pest of maize crops we tried to obtain successive generations under environment controlled of the insect mentioned. In this paper we aim to improve the efficiency of the multiplication of the species with respect to two aspects: attending larval and multiplication high efficiency. The coefficient value of 20, 90 obtained by us indicate a considerable increase in performance compared to previous attempts multiplication thus increasing your chances of recovery of this method of mass multiplication.

  9. Interaction of Insecticide and Media Moisture on Ambrosia Beetle (Coleoptera: Curculionidae) Attacks on Selected Ornamental Trees.

    Science.gov (United States)

    Frank, Steven D; Anderson, Amanda L; Ranger, Christopher M

    2017-12-08

    Exotic ambrosia beetles, particularly Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae: Scolytinae) and Xylosandrus germanus (Blandford) (Coleoptera: Curculionidae: Scolytinae), are among the most damaging pests of ornamental trees in nurseries. Growers have had few tactics besides insecticide applications to reduce ambrosia beetle attacks but recent research has shown that attacks may be reduced by maintaining media moisture below a 50% threshold thereby reducing flood stress. We compared the efficacy of managing media moisture and insecticide applications for reducing ambrosia beetle attacks on three ornamental tree species in North Carolina. During trials in spring 2013 and 2015, flooded Cornus florida and Cornus kousa were heavily attacked despite sprays with permethrin, but nonflooded C. kousa or C. florida were not attacked. In spring 2015 trials, both nonflooded and flooded Styrax japonicus were heavily attacked regardless of permethrin applications. Although ethanol emissions were not measured, the apparently healthy nonflooded S. japonicus trees may have been exposed to an unknown physiological stress, such as low temperature injury, the previous winter, which predisposed them to beetle attack. However, ethanol levels within host tissues were not measured as part of the current study. X. crassiusculus (75%), Xyloborinus saxesenii Ratzburg (13%), and X. germanus (9%) were the most abundant species collected in ethanol baited traps deployed in 2015, while X. crassiusculus (63%) and X. germanus (36%) were the predominant species reared from attacked trees. Results indicate that managing media moisture levels at or below 50%, and maximizing tree health overall, may provide significant protection against Xylosandrus spp. attacks in flood intolerant tree species. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  10. Estudo da descoloração do corante FD&C azul no 2 Indigotina pelo tratamento combinado do fungo Trametes versicolor e processo de filtração lenta

    OpenAIRE

    Lopes, Maria Margareth Gonçalves; Sales, Paulo de Tarso Ferreira; Campos, Luiza Cintra; Schimidt, Fernando; Santiago, Mariângela Fontes

    2014-01-01

    O uso de fungos na descoloração de corantes com métodos economicamente viáveis de produção de água bacteriologicamente segura há muito vem sendo descrito por diversos autores. Este trabalho teve por objetivo investigar a eficiência da remoção de corante artificial FD&C azul no 2 Indigotina, com uso do fungo de degradação branca Trametes versicolor em combinação com a filtração lenta. Para a realização dos trabalhos, foram instalados dois protótipos de filtros lentos denominados FL-A e FL-B - ...

  11. Locomotor activity of Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae on Chilean sandy beaches Actividad locomotora de Phalerisida maculata Kulzer (Coleoptera, enebrionidae en playas arenosas chilenas

    Directory of Open Access Journals (Sweden)

    EDUARDO JARAMILLO

    2000-03-01

    Full Text Available The locomotor activity of the beetle Phalerisida maculata Kulzer (Coleoptera Tenebrionidae was studied on the surface of the substrate in two sandy beaches of the Chilean coast: one in north central (ca. 29ºS and the other in south central Chile (ca.39ºS. During the summer period of 1991 the circadian locomotor activity was studied in the southern beach, while during that of 1997 in both beaches. To analyze the activity, pitfall traps were used which were ordered along two transects extended between the upper beach and the resurgence zone. The traps were checked (i.e. collection of captured insects every two hours for a total period of 26 hours. The results showed that the adults of P. maculata were mostly active during the night hours, whereas the larvae were active during both, the day and night. Studies carried out in the beach located in south central Chile show that differences in the tidal range (neap vs. spring tides do not affect the activity patterns. During the locomotor activity, adult and larvae move to lower intertidal levels than those usually occupied while buried. Results of laboratory experiments using actographs under conditions of darkness and constant temperature, suggest that adults and larvae of P. maculata presented a circadian rhythm similar to that observed in the field experiments. It is concluded that P. maculata presents a behaviour that appear to be under control of an endogenous rhythm, without showing differences in the circadian rhythm of activity when beaches located at different latitudes are comparedSe estudió la actividad locomotriz del escarabajo Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae sobre la superficie del sustrato de dos playas arenosas de la costa de Chile: una en el centro norte (ca. 29ºS y otra en el centro sur (ca. 39ºS. Durante el período estival de 1991 se estudió la actividad locomotriz circadiana en la playa del centro sur y durante el de 1997 en ambas playas. Para analizar

  12. Desenvolvimento de Podisus nigrispinus (Dallas (Heteroptera, Pentatomidae com Zophobas confusa Gebien (Coleoptera, Tenebrionidae comparado à duas outras presas alternativas Development of Podisus nigrispinus (Dallas (Heteroptera, Pentatomidae fed with Zophobas confusa Gebien (Coleoptera, Tenebrionidae compared with two another alternative preys

    Directory of Open Access Journals (Sweden)

    Teresinha V Zanuncio

    1996-01-01

    Full Text Available Nymphs of Podisus nigrispinus (Dallas, 1851 were fed with Zophobas confusa Gebien, 1906, Tenebrio molitor Linnaeus, 1758 (Coleoptera, Tenebrionidae and Musca domestica Linnaeus, 1758 (Diptera, Muscidae under 25±0.5ºC, 60±10% of RH and photophase of 12h. Nymphal viability of P. nigrispinus were 64.0%, 80.0% and 92.0% with Z. confusa, M. domestica and T. molitor, respectively. No differences were found for pre-oviposition period, number of egg masses, number of eggs, egg viability, and longevity for females of this predator fed with any of these preys.

  13. Morphological Diversity of Coleoptera (Arthropoda: Insecta in Agriculture and Forest Systems

    Directory of Open Access Journals (Sweden)

    Pâmela Niederauer Pompeo

    2017-11-01

    Full Text Available ABSTRACT Coleopterans (Coleoptera are major ecosystem service providers. However ecomorphological features that are comparable in a wide range of invertebrates within this group and in various environments must be found, to be able to study regions with different species, contributing to overcome difficulties of the taxonomic approach and understand the functioning of ecosystems. This research addressed the diversity of Coleoptera, using a methodology of ecomorphological traits, as well as their relation with the land use systems (LUS and the soil properties. The following LUS were evaluated: no-tillage (NT, crop-livestock integration (CLI, pasture (PA, Eucalyptus stands (EST, and native forest (NF. Samples were collected using a 3 × 3 point grid (sampling points at a distance of 30 m, in winter and summer, in three municipalities on the Southern Santa Catarina Plateau, Brazil. Coleopterans were collected using the methodology recommended by the Tropical Soil Biology and Fertility Program, based on the excavation of soil monoliths, and on pitfall traps. To evaluate the biological forms (morphotypes and ecomorphological groups, the ecomorphological index (EMI methodology was adopted and the modified soil biological quality (SBQ index was determined. At the same points, samples were collected to evaluate environmental variables (soil physical, chemical, and microbiological properties. Density data underwent nonparametric univariate statistical analysis and multivariate abundance to verify the distribution of coleopterans in the LUS, and the environmental variables were considered as explanatory. Regardless of the LUS, 14 morphotypes were identified, and adult coleopterans with epigean morphologic adaptations were more abundant than hemi-edaphic and edaphic coleopterans, respectively. Morphotype diversity was higher in the systems NF, EST, and PA in summer and in NT in winter. The reductions in SBQ index were not associated with a gradient of land

  14. Avaliação de inseticidas no controle de Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae e Rhyzopertha dominica (Fabricius (Coleoptera, Bostrichidae em trigo armazenado Efficacy of insecticides to control Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae and Rhyzopertha dominica (Fabricius (Coleoptera, Bostrichidae in stored wheat

    Directory of Open Access Journals (Sweden)

    Rui S. Furiatti

    1999-03-01

    Full Text Available The efficiency of insecticides against Sitophilus oryzae (Linnaeus, 1763 (Coleoptera, Curculionidae and Rhyzopertha dominica (Fabricius, 1792 (Coleoptera, Bostrichidae was determined exposing them to treated wheat in laboratory. Each plot, which was composed by 1.2 kg of wheat grain, was treated with 5 ml of the concentration/kg of wheat using a propelling air atomizer. Fifteen days after treatment 50 g samples of the grain were taken, from each plot, placed in plastic jars into which 10 adult insects of each species were released. This was repeated every 30 days until 165 days after treatment. The number of dead insects was counted 15 days after each infestation thus the evaluations were made at 30, 60, 90, 120, 150 and 180 days after the insecticide application. Esfenvalerate+fenitrothion+pyperonil butoxide (0,375+7,5+3,0 and 0,5+10,0+4,0 g a.i./t, fenitrothion+esfen-valerate (7,5+0,375 and 10,0+0,375 g a.i./t, fenitrothion+synergised deltamethrin (7,5+0,375 g a.i./t and fenitrothion (7,5 g a.i./t were effective against Sitophilus oryzae until 180 days after treatment. Esfenvalerate+fenitrothion+pyperonil butoxide (0,5+10,0+4,0 g a.i./t, fenitrothion+esfenvalerate (7,5+0,375 and 10,0+0,375 g a.i./t, fenitrothion+synergised deltamethrin (7,5+0,375 g a.i./t were effective against R. dominica until 180 days after treatment. Synergised deltamethrin (0,375 g a.i./t was effective against S. oryzae until 150 days and against R. dominica until 120 days after treatment. Esfenvalerate (0,5 g a.i./t was ineffective against S. oryzae and effective until 60 days after treatment against R. dominica. Fenitrothion (7,5 g a.i./t was ineffective against R. dominica. The use of mixtures of organophosphorus plus pyrethroids were more effective against S. oryzae and R. dominica when compared with these insecticides used alone.

  15. Limonene: attractant kairomone for white pine cone beetles (Coleoptera: Scolytidae) in an Eastern white pine seed orchard in Western North Carolina

    Science.gov (United States)

    Daniel R. Miller

    2007-01-01

    I report on the attraction of the white pine cone beetle, Canophthorus coniperda (Schwarz) (Coleoptera: Scolytidae), to traps baited with the host monoterpene limonene in western North Carolina. Both (+)- and (-)-limonene attracted male and female cone beetles to Japenese beetle traps in an eastern white pine, Pinus strobus L. seed...

  16. Efficacy of “Verbenone Plus” for protecting ponderosa pine trees and stands from Dendroctonus brevicomis (Coleoptera: Curculionidae) attack in British Columbia and California

    Science.gov (United States)

    Christopher J. Fettig; Stephen R. McKelvey; Christopher P. Dabney; Dezene P.W. Huber; Cameron C. Lait; Donald L Fowler; John H. Borden

    2012-01-01

    The western pine beetle, Dendroctonus brevicomis LeConte (Coleoptera: Curculionidae, Scolytinae), is a major cause of ponderosa pine, Pinus ponderosa Douglas ex Lawson, mortality in much of western North America. We review several years of research that led to the identification of Verbenone Plus, a novel four-component...

  17. A new species of myrmecophilous lady beetle in the genus Diomus (Coleoptera: Coccinellidae: Diomini) from Chiapas, Mexico that feeds on green coffee scale, Coccus viridis (Green) (Hemiptera: Coccidae)

    Science.gov (United States)

    A new species of myrmecophilous lady beetle in the genus Diomus (Coleoptera: Coccinellidae: Diomini) is described from a coffee agroecosystem in Chiapas, Mexico. The new species was found preying on the green coffee scale pest, Coccus viridis (Green), tended primarily by Azteca sericeasur Longino an...

  18. Entomopathogenic fungi as a biological control agents for the vector of the laurel wilt disease, the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae)

    Science.gov (United States)

    The redbay ambrosia beetle (RAB), Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae) vectors the fungal pathogen, Raffaelea lauricola, which causes laurel wilt (LW), a lethal disease of trees in the family Lauraceae, including the most commercially important crop in this family, avocado, Pe...

  19. Evaluating the virulence and longevity of non-woven fiber bands impregnated with Metarhizium anisopliae against the Asian longhorned beetle, Anoplophora glabripennis (Coleoptera: Cerambycidae)

    Science.gov (United States)

    Ryan P. Shanley; Melody Keena; Micheal M. Wheeler; Jarrod Leland; Ann E. Hajek

    2009-01-01

    Fiber bands impregnated with entomopathogenic fungi (=fungal bands) provide an effective method for controlling the invasive Asian longhorned beetle, Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae). In this study we investigated the effective longevity of fungal bands for use against A. glabripennis, using...

  20. Descrição da larva de último instar e pupa de Epacroplon cruciatum (Aurivillius (Coleoptera, Cerambycidae, Cerambycinae e notas biológica

    Directory of Open Access Journals (Sweden)

    Juares Fuhrmann

    2012-03-01

    Full Text Available Descrição da larva de último instar e pupa de Epacroplon cruciatum (Aurivillius (Coleoptera, Cerambycidae, Cerambycinae e notas biológicas. Último instar larval e pupa do Hexoplonini sul-americano Epacroplon cruciatum (Aurivillius, 1899 são descritos, ilustrados e disponibilizadas notas biológicas.

  1. Attraction of the southern pine beetle, Dendroctonus frontalis, to pheromone components of the western pine beetle, Dendroctonus brevicomis (Coleoptera: Curculionidae: Scolytinae), in an allopatric zone

    Science.gov (United States)

    Deepa S. Pureswaran; Richard W. Hofstetter; Brian T. Sullivan

    2008-01-01

    Subtle differences in pheromone components of sympatric species should be attractive only to the producing species and unattractive or repellent to the nonproducing species, and thereby maintain reproductive isolation and reduce competition between species. Bark beetles Dendroctonus brevicomis and D. frontalis (Coleoptera: Curculionidae) are known to...

  2. Distribution of trunk-injected 14C-imidacloprid in ash trees and effects on emerald ash borer (Coleoptera: Buprestidae) adults

    Science.gov (United States)

    David Mota-Sánchez; Bert M. Cregg; Deborah G. McCullough; Therese M. Poland; Robert M. Hollingworth

    2009-01-01

    The emerald ash borer (EAB), Agrilus planipennis (Coleoptera: Buprestidae) is a destructive exotic pest of North American ash (Fraxinus sp.) trees. Trunk injection of imidacloprid is commonly used to protect landscape ash trees from A. planipennis damage. Efficacy can vary and little is known about the...

  3. Effectiveness of bifenthrin (Onyx) and carbaryl (Sevin SL) for protecting individual, high-value conifers from bark beetle attack (Coleoptera: Curculionidae: Scolytinae) in the Western United States

    Science.gov (United States)

    Christopher J. Fettig; Kurt K. Allen; Robert R. Borys; John Christopherson; Christopher P. Dabney; Thomas J. Eager; Kenneth E. Gibson; Elizabeth G. Hebertson; Daniel F. Long; A. Steven Munson; Patrick J. Shea; Sheri L. Smith; Michael I. Haverty

    2006-01-01

    High-value trees, such as those located in residential, recreational, or administrative sites, are particularly susceptible to bark beetle (Coleoptera: Curculionidae: Scolytinae) attack as a result of increased amounts of stress associated with drought, soil compaction, mechanical injury, or vandalism. Tree losses in these unique environments generally have a...

  4. Evaluation of lure combinations containing essential oils and volatile spiroketals for detection of host-seeking Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae)

    Science.gov (United States)

    The invasive redbay ambrosia beetle, Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae) vectors the fungal pathogen (Raffaelea lauricola) that causes laurel wilt, a disease responsible for widespread mortality of trees in the Lauraceae in the southeastern U.S. Early detection of in...

  5. Effectiveness of Cedar Oil Products for Preventing Host Use by Ips avulsus (Eichhoff) (Coleoptera: Curculionidae) in a Modified Small-Bolt Assay

    Science.gov (United States)

    B. L. Strom; L. M. Roton

    2011-01-01

    Insecticide products based on cedar oil are readily available, but evaluations against pine bark beetles (Coleoptera: Curculionidae: Scolytinae) are lacking. In the southeastern U.S., the southern pine beetle, Dendroctonus frontalis Zimm, is the major bark beetle pest for which tree protectants are applied. However, Ips avulsus (Eichhoff) are more consistently...

  6. New records of Paracrias Ashmead (Hymenoptera, Eulophidae as parasitoids on weevil larvae (Coleoptera, Curculionidae in Brazil, with the description of a new species

    Directory of Open Access Journals (Sweden)

    Luciano Palmieri

    2013-09-01

    Full Text Available Paracrias strii Schauff, 1985 and P. ceratophaga Palmieri & Hansson sp. nov. are first record in Brazil and both are associated with Ceratopus Schoenherr larvae (Coleoptera, Curculionidae reared from syconia of two species of fig-trees. Both Paracrias species are diagnosed and illustrated. Males of P. ceratophaga sp. nov. are described. The association of Paracrias with weevil larvae is briefly discussed.

  7. Orientation behavior of the predator Laricobius nigrinus (Coleoptera: Derodontidae) to hemlock woolly adelgid and host tree odors in a multi-chambered olfactometer

    Science.gov (United States)

    Kimberly F. Wallin; Tanya M. Latty; Darrell W. Ross

    2011-01-01

    We studied the adult ambulatory response of the predator, Laricobius nigrinus Fender (Coleoptera: Derodontidae), to odors from its prey, Adelges tsugae Annand, the hemlock woolly adelgid, and foliage of hemlock woolly adelgid, host hemlocks (Tsuga spp.), and other conifers. Both the predator and hemlock woolly...

  8. Behavioral responses of Laricobius spp.and hybrids (Coleoptera: Derodontidae) to hemlock woolly adelgid and adelgid host tree odors in an olfactometer

    Science.gov (United States)

    Arielle L. Arsenault; Nathan P. Havill; Albert E. Mayfield; Kimberly F. Wallin

    2015-01-01

    The predatory species Laricobius nigrinus (Fender) and Laricobius osakensis (Shiyake and Montgomery) (Coleoptera: Derodontidae) have been released for biological control of hemlock woolly adelgid (Adelges tsugae; Hemiptera: Adelgidae) in eastern North America. L. osakensis is native to Japan, whereas L. nigrinus is endemic to the Pacific Northwest of the United States...

  9. A new species of oobius trjapitzin (hymenoptera:encyrtidae) from the russian far east that parasitizes eggs of emerald ash borer (coleoptera:buprestidae)

    Science.gov (United States)

    A new egg parasitoid of the emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) from the Russian Far East, Oobius primorskyensis Yao et Duan is described. Both morphological characters and analysis of DNA sequence divergence suggest that this species is different from the previ...

  10. Comparison of male and female emerald ash borer (Coleoptera: Buprestidae) responses to phoebe oil and (Z)-3-hexanol lures in light green prism traps

    Science.gov (United States)

    Gary G. Grant; Therese M. Poland; Tina Ciaramitaro; D. Barry Lyons; Gene C. Jones

    2011-01-01

    We conducted trapping experiments for the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) in Michigan, USA, and Ontario, Canada, to compare unbaited light green sticky prism traps with traps baited with phoebe oil, (Z)-3-hexenol (Z3-6:OH), or blends of other green leaf volatiles (GLVs) with Z3-6:OH. Traps were placed in the...

  11. Xyleborus bispinatus (Coleoptera: Curculionidae) reared on artificial media using sawdust from avocado or silkbay in presence or absence of the laurel wilt pathogen (Raffaelea lauricola).

    Science.gov (United States)

    Xyleborus bispinatus Eichhoff (Coleoptera: Curculionidae) was reported in Florida for the first time in 2013. Previously, it was unrecognized and not distinguished from the morphologically similar Xyleborus ferrugineus (F.). Like other members of the tribe Xyleborini, X. ferrugineus (and possibly X....

  12. Seasonal dynamics of saproxylic beetles (Coleoptera occurring in decaying birch (Betula spp. wood in the Kampinos National Park

    Directory of Open Access Journals (Sweden)

    Sawoniewicz Michał

    2015-09-01

    Full Text Available The aim of the study was to identify the seasonal changes in the number of saproxylic beetles connected with birch in the Kampinos National Park. The research was conducted for 12 consecutive months in research areas representing 10 different site types. The beetles were collected from wood using photoeclectors. The largest number of species was collected in April and the lowest in January. An increase in number occurred during spring and summer months for species associated only with rotting wood, fructifications of tree fungi, the subcortical environment and hollows. In the same period the number of species not associated or potentially associated with decaying trees and wood decreased. During winter months, the differences in the number of trapped specimens were the smallest. The proportion of zoophagous species amongst the collected specimen increased in autumn and winter. The share of saprophagous species was the highest during the summer-autumn period and the share of mycetophages (jointly with myxomycophages was the highest during spring and summer. We distinguished two separate groups of Coleoptera with the first one (‘summer group’ including species trapped during late-spring and summer months, while the second one (‘winter group’ includes species found in autumn, winter and early-spring months. In the ‘summer group’, an average of 55.8 species was trapped each month with 331.2 specimen of Coleoptera, while in the ʻwinter group’ an average of 56.1 species with 228.4 Coleoptera specimen were caught.

  13. Influence of 60Co gamma radiation on Zabrotes subfasciatus (Bohemann, 1833) (Coleoptera, Curculionidae) and beams Phaseolus vulgaris

    International Nuclear Information System (INIS)

    Moraes Rego, A.F. de; Rodrigues, Z.A.; Oliveira, M.L. de; Santana, M.D.

    1986-01-01

    Influence of gamma radiation on Zabrotes subfasciatus (Boh, 1833) (Coleoptera Bruchidae) and the beans Phaseolus vulgaris (L.). The effects of 60 CO gamma radiation, 50 Gy, on both Phaseolus vulgaris (L.) seedbeans and adults of Zabrotes subfasciatus were studied using the no free choise method. Radiation decreased insect fertility hence insect population and it damage loss of weight and germination of seedbeans. However, radiation resulted in abnamal seedlings, showing various degrees of morphological malformation, although there was no effect on germination rates or seedling vigor. (Author) [pt

  14. Review of the genus Fontidessus Miller & Spangler, 2008 (Coleoptera, Dytiscidae, Hydroporinae, Bidessini with description of four new species

    Directory of Open Access Journals (Sweden)

    Kelly Miller

    2014-07-01

    Full Text Available The genus Fontidessus Miller & Spangler, 2008 (Coleoptera: Dytiscidae: Hydroporinae: Bidessini is reviewed. The genus now includes seven species with three previously described, and four new species described here: F. microphthalmus Miller & Montano, sp. n.; F. bettae Miller & Montano, sp. n.; F. christineae Miller & Montano, sp. n., and F. aquarupe Miller & Montano, sp. n. Each species is diagnosed and described, including the previously known species, based on new specimens and new information. Habitus, male genitalia and other diagnostic features are illustrated for each species. A key to the seven species is provided. Fontidessus species are unique to hygropetric habitats in the Guiana Shield craton of northern South American.

  15. EFECTO DE LA GLUCOSA Y NITRATO DE AMONIO SOBRE LAS ENZIMAS LIGNINOLÍTICAS PRODUCIDAS POR Trametes versicolor INMOVILIZADO EN ESPUMA Y LA DECOLORACIÓN DE UN EFLUENTE PAPELERO EN UN BIORREACTOR DE LECHO FLUIDIZADO

    Directory of Open Access Journals (Sweden)

    M. Martínez-Salgado

    2005-12-01

    Full Text Available En Colombia se han realizado varios estudios para el tratamiento de aguas residuales de la industria papelera a escala de laboratorio, con el hongo Trametes versicolor inmovilizado en espuma de poliuretano; este hongo ha sido ampliamente estudiado por la producción de enzimas ligninolíticas y su potencial uso en la decoloración de efluentes. En este trabajo, se estudió el efecto de una fuente de carbono (glucosa y una de nitrógeno (nitrato de amonio en un biorreactor de lecho fluidizado, con adición al medio de cultivo de 0.02% (v/v de Tween 80, 12 ppm de MnSO4 y 1 mM de CuSO4, desarrollando un diseño factorial 22 con dos diferentes concentraciones de los nutrientes, siendo éstos las variables independientes y las variables dependientes fueron la actividad enzimática, las unidades de color y el consumo de sustrato los cuales fueron determinados por métodos colorimétricos, además de la biomasa y pH. El uso del biorreactor de lecho fluidizado con entrada de aire constante, arrojó datos importantes sobre la decoloracióny la producción de enzimas en el agua residual no estéril. Los máximos valores de la enzima Mnperoxidasa fueron hallados en las concentraciones altas y bajas de glucosa y nitrato de amonio con valores de 9.257U y 6.502U; para la enzima Lacasa, al adicionar separadamente las dos variables se obtuvo valores de 8.618U y 8.341U. Las enzimas fueron relacionadas con la disminución de las unidades de color hasta 481.06UC, junto con el aumento de la biomasa del hongo Trametes versicolor hasta 3.365 g junto con flora nativa del agua residual. Los resultados encontrados, determinaron que las variables de consumo de sustrato y biomasa, tienen una gran importancia debido a que el aumento de la biomasa genera un aumento en la actividad de la enzima MnP y en la reducción de las unidades de color.

  16. Ambrosia Beetle (Coleoptera: Scolytidae) Species, Flight, and Attack on Living Eastern Cottonwood Trees.

    Energy Technology Data Exchange (ETDEWEB)

    Coyle, D R; D.C. Booth: M.S. Wallace

    2005-12-01

    ABSTRACT In spring 2002, ambrosia beetles (Coleoptera: Scolytidae) infested an intensively managed 22-ha tree plantation on the upper coastal plain of South Carolina. Nearly 3,500 scolytids representing 28 species were captured in ethanol-baited traps from 18 June 2002 to 18 April 2004. More than 88% of total captures were exotic species. Five species [Dryoxylon onoharaensum (Murayama), Euwallacea validus (Eichhoff), Pseudopityophthorus minutissimus (Zimmermann), Xyleborus atratus Eichhoff, and Xyleborus impressus Eichhoff]) were collected in South Carolina for the first time. Of four tree species in the plantation, eastern cottonwood, Populus deltoides Bartram, was the only one attacked, with nearly 40% of the trees sustaining ambrosia beetle damage. Clone ST66 sustained more damage than clone S7C15. ST66 trees receiving fertilization were attacked more frequently than trees receiving irrigation, irrigation_fertilization, or controls, although the number of S7C15 trees attacked did not differ among treatments. The study location is near major shipping ports; our results demonstrate the necessity for intensive monitoring programs to determine the arrival, spread, ecology, and impact of exotic scolytids.

  17. The coastal rove beetles (Coleoptera, Staphylinidae of Atlantic Canada: a survey and new records

    Directory of Open Access Journals (Sweden)

    Christopher Majka

    2008-09-01

    Full Text Available The coastline inhabiting rove beetles (Coleoptera: Staphylinidae of Atlantic Canada are surveyed. Thirty-three species have now been recorded in Atlantic Canada including 26 in New Brunswick, 15 in Newfoundland, 31 in Nova Scotia, and 13 on Prince Edward Island. Oligota parva Kraatz, Acrotona avia (Casey, Strigota ambigua (Erichson, and Myrmecopora vaga (LeConte, are all newly recorded in Canada, and Bledius mandibularis Erichson is newly recorded in Atlantic Canada. We retain A. avia as a species distinct from A. subpygmaea Bernhauer and designate a lectotype and paralectotypes for A. avia. Ten new provincial records are reported, one from New Brunswick, six from Nova Scotia, and three from Prince Edward Island. Four functional groups, halobiont (obligate, halophile (facultative, haloxene (tolerant, and incidental coastal species, are distinguished and the fauna is examined from the perspective of the particular coastline habitats and microhabitats they have been found to inhabit. Fourteen of the 33 staphylinids are introduced, Palearctic species, and eight of these have been associated with historic dry ballast shipping to the region from Great Britain. A trophic analysis indicates that some species are phytophagous algae feeders, while others are either generalist predators, or predators specializing on particular taxonomic or functional groups of invertebrates. Finally, some attention is devoted to discussing the diminished areas of coastline environments such as coastal marshes, and the various kinds of environmental disturbances and degradations they have experienced. These indicate the potential vulnerability of such coastal habitats and consequently of the communities of beetles that inhabit them.

  18. Ecological, morphological, and histological studies on Blaps polycresta (Coleoptera: Tenebrionidae) as biomonitors of cadmium soil pollution.

    Science.gov (United States)

    Osman, Wafaa; El-Samad, Lamia M; Mokhamer, El-Hassan; El-Touhamy, Aya; Shonouda, Mourad

    2015-09-01

    Soil pollution in Egypt became far more serious than before due to either the heavy usage of different toxic pesticides or aerosol deposition of industrial pollutants. The present mentioned ground beetle, Blaps polycresta Tschinkel 1975 (Coleoptera: Tenebrionidae), showed ecological, morphological, and histological alterations in adult insects as biomonitors. Two cultivated sites (reference and polluted) were chosen for sampling the insects. The results indicated a significant increase in soil cadmium concentration of the polluted site leading to sex-specific difference in cadmium accumulation in gonads and alimentary canal of insects that being higher in males than females. The cadmium pollution leads significantly to a decrease in population density, a reduction in body weight, an increase in mortality rate, and an increase in sex ratio of the insects. The results also revealed a striking decrease in body length of the polluted insects with a marked increase in the percentage of deformed gonads and alimentary canal of both sexes. Some histopathological alterations were also recorded in testis, ovary, and midgut of the polluted insects. Our results confirmed that beetles are a good bioindicator for soil pollution, and the different studied parameters could be easily employed as sensitive monitors for cadmium soil pollution.

  19. Volatiles released from Vaccinium corymbosum were attractive to Aegorhinus superciliosus (Coleoptera: Curculionidae) in an olfactometric bioassay.

    Science.gov (United States)

    Parra, Leonardo; Mutis, Ana; Ceballos, Ricardo; Lizama, Marcelo; Pardo, Fernando; Perich, Fernando; Quiroz, Andrés

    2009-06-01

    The objective of this study was to evaluate the role of host volatiles in the relationship between a blueberry plant Vaccinium corymbosum L. and the raspberry weevil Aegorhinus superciliosus (Guérin) (Coleoptera: Curculionidae), the principal pest of blueberry in the south of Chile. Volatiles from the aerial part of different phenological stages of the host were collected on Porapak Q and analyzed by coupled gas chromatography-mass spectrometry (GC-MS). Several chemical groups were identified including green leaf volatiles, aromatic compounds, and terpenes. The olfactometric responses of A. superciliosus toward different odor sources were studied in a four-arm olfactometer. Blueberry shoots at the phenological stages of fruit set, and blue-pink fruit color elicited the greatest behavioral responses from weevils. Five compounds (2-nonanone, eucalyptol, R- and S-limonene, and 4-ethyl benzaldehyde) elicited an attractant behavioral response from A. superciliosus. The results suggest the host location behavior of A. superciliosus could be mediated by volatiles derived from V. corymbosum. This work has identified a number of compounds with which it is possible to develop a lure for the principal pest of blueberry in southern Chile.

  20. Odor-baited trap trees: a novel management tool for plum curculio (Coleoptera: Curculionidae).

    Science.gov (United States)

    Leskey, Tracy C; Piñero, Jaime C; Prokopy, Ronald J

    2008-08-01

    The plum curculio, Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), one of the most important pests of apple (Malus spp.) in eastern and central North America, historically has been managed in New England apple orchards by three full block insecticide applications. Efforts to reduce insecticide inputs against plum curculio include perimeter row sprays, particularly after petal fall, to control immigrating adults. The odor-baited trap tree approach represents a new reduced input strategy for managing plum curculio based on the application of insecticides to a few perimeter-row trap trees rather than the entire perimeter row or full orchard block. Here, we compared the efficacy of a trap tree approach with perimeter row treatments to manage populations after petal fall in commercial apple orchards in 2005 and 2006. Injury was significantly greater in trap trees compared with unbaited perimeter row treated trees in both years of the study. In 2005, heavy rains prevented growers from applying insecticide applications at regular intervals resulting in high injury in nearly all blocks regardless of type of management strategy. In 2006, both the trap-tree and perimeter-row treatments prevented penetration by immigrating populations and resulted in economically acceptable levels of injury. The trap tree management strategy resulted in a reduction of approximately 70% total trees being treated with insecticide compared with perimeter row sprays and 93% compared with standard full block sprays.

  1. Impact of Cultivation and Subsequent Burial on Cydia pomonella (Lepidoptera: Tortricidae) and Conotrachelus nenuphar (Coleoptera: Curculionidae).

    Science.gov (United States)

    Baughman, William B; Nelson, Peter N; Grieshop, Matthew J

    2015-06-01

    We assessed the efficacy of cultivation as a potential management strategy for codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), and plum curculio, Conotrachelus nenuphar Herbst (Coleoptera: Curculionidae) in apple orchards. Cocooned codling moth pupae and thinning apples infested with plum curculio larvae were cultivated over in the field. Emergence, percent burial, damage to buried fruit, and depth of burial was recorded. In the laboratory, both insects were buried at variable depths in sand and potting soil and emergence was measured. A greater proportion of plum curculio larvae buried in infested fruit under laboratory conditions survived to adulthood compared with unburied infested fruit, down to 15 cm. No codling moth adults emerged from under 1 cm or more of sand. Buried codling moth larvae experienced drastically reduced survival to adulthood compared with unburied larvae. These results indicate that strip cultivation may negatively impact codling moth diapausing larvae and pupae on the ground, but not likely to negatively impact plum curculio in infested dropped apples. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America.

  2. Comparison of leaf beetle assemblages of deciduous trees canopies in Hungary (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Vig, K; Markó, V

    2005-01-01

    The species richness and species composition of Coleoptera assemblages were investigated in deciduous tree canopies in Hungary. Apple and pear orchards were investigated in Nagykovácsi, Kecskemét and Sárospatak in 1990-94, and limes and maples in Keszthely in 1999-2002. Faunistic results and conclusions of these investigations were published elsewhere. Examination of the fauna of parks, avenues and other planted urban plant stocks has only begun to occupy researchers in the last decade in Hungary. The proportion of leaf-beetle species in the material gathered on maples and limes ranged between 17.0 and 21.3 per cent. The commonest leaf-beetle specimens collected in the lime canopy were Aphthona euphorbiae, Chaetocnema tibialis, Longitarsus lycopi, L. pellucidus, L. pratensis and L. succineus. The commonest on maple were Aphthona euphorbiae, Chaetocnema concinna, C. tibialis, Longitarsus lycopi, L. pellucidus, L. succineus, Phyllotreta cruciferae and P. vittula. This study presents the details on the composition of the chrysomelid communities that was compared by metric ordination using the Syntax 5.1 program.

  3. Temperature-dependent development and emergence pattern of Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae) from coffee berries.

    Science.gov (United States)

    Jaramillo, Juliana; Chabi-Olaye, Adenirin; Borgemeister, Christian

    2010-08-01

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae), is the most important constrain for coffee production throughout the world. Knowledge on the emergence pattern of H. hampei females to infest new berries is crucial to effectively plan control measures. In this laboratory study, we assessed the development of immature stages and the emergence pattern of H. hampei females from the berries by exposing them to temperatures that are typical for high-altitude plantations (> or = 1,700 m above sea level [masl] ) or when coffee is grown under shade trees (20-22 degrees C), and optimum altitude plantations (1,200-1,600 masl) or nonshaded coffee (25-30 degrees C). Fecundity and emergence pattern of H. hampei females from coffee berries varied with temperature. Temperature played a crucial role determining the rate of H. hampei development and therefore the emergence of the females to start a new infestation cycle. The emergence and colonization phases of new colonizing females in coffee plantations with mean temperatures of 20, 25, or 30 degrees C would take place at different moments in the development of the coffee berries, and in some cases more than once. The implications of our findings for an improved, site-specific timing of control interventions against H. hampei are discussed.

  4. Erotylidae (Insecta, Coleoptera of Poland – problematic taxa, updated keys and new records

    Directory of Open Access Journals (Sweden)

    Rafal Ruta

    2011-10-01

    Full Text Available New data concerning the occurrence of pleasing fungus beetles (Coleoptera: Erotylidae in Poland are given, with a focus on rare and difficult to identify Central European taxa. Cryptophilus cf. integer (Heer (Cryptophilinae is reported from the Polish territory for the first time based on adult and larval specimens collected in the Wielkopolska-Kujawy Lowland. Identification problems concerning species of Cryptophilus introduced to Europe are discussed. Triplax carpathica Reitter (Erotylinae is recorded from the Białowieża Primeval Forest, which is the first known non-Carpathian finding of this species, located in the close proximity of the Polish-Belarussian UNESCO World Heritage Site “Białowieża Forest”. Discussion of T. carpathica being conspecific with Siberian T. rufiventris Gebler is provided. New Polish localities of several other Erotylidae are reported, and an updated key to Central European species of Triplax is given. The Triplax key is supplemented with dorsal and ventral habitus images of all treated Triplax species. One of the rarest Central European erotyline species Combocerus glaber (Schaller is recorded from xerothermic grasslands in North-West Poland.

  5. Erotylidae (Insecta, Coleoptera) of Poland - problematic taxa, updated keys and new records.

    Science.gov (United States)

    Ruta, Rafał; Jałoszyński, Paweł; Sienkiewicz, Paweł; Konwerski, Szymon

    2011-01-01

    New data concerning the occurrence of pleasing fungus beetles (Coleoptera: Erotylidae) in Poland are given, with a focus on rare and difficult to identify Central European taxa. Cryptophilus cf. integer (Heer) (Cryptophilinae) is reported from the Polish territory for the first time based on adult and larval specimens collected in the Wielkopolska-Kujawy Lowland. Identification problems concerning species of Cryptophilus introduced to Europe are discussed. Triplax carpathica Reitter (Erotylinae) is recorded from the Białowieża Primeval Forest, which is the first known non-Carpathian finding of this species, located in the close proximity of the Polish-Belarussian UNESCO World Heritage Site "Białowieża Forest". Discussion of Triplax carpathica being conspecific with Siberian Triplax rufiventris Gebler is provided. New Polish localities of several other Erotylidae are reported, and an updated key to Central European species of Triplax is given. The Triplax key is supplemented with dorsal and ventral habitus images of all treated Triplax species. One of the rarest Central European erotyline species Combocerus glaber (Schaller) is recorded from xerothermic grasslands in North-West Poland.

  6. Erotylidae (Insecta, Coleoptera) of Poland – problematic taxa, updated keys and new records

    Science.gov (United States)

    Ruta, Rafał; Jałoszyński, Paweł; Sienkiewicz, Paweł; Konwerski, Szymon

    2011-01-01

    Abstract New data concerning the occurrence of pleasing fungus beetles (Coleoptera: Erotylidae) in Poland are given, with a focus on rare and difficult to identify Central European taxa. Cryptophilus cf. integer (Heer) (Cryptophilinae) is reported from the Polish territory for the first time based on adult and larval specimens collected in the Wielkopolska-Kujawy Lowland. Identification problems concerning species of Cryptophilus introduced to Europe are discussed. Triplax carpathica Reitter (Erotylinae) is recorded from the Białowieża Primeval Forest, which is the first known non-Carpathian finding of this species, located in the close proximity of the Polish-Belarussian UNESCO World Heritage Site “Białowieża Forest”. Discussion of Triplax carpathica being conspecific with Siberian Triplax rufiventris Gebler is provided. New Polish localities of several other Erotylidae are reported, and an updated key to Central European species of Triplax is given. The Triplax key is supplemented with dorsal and ventral habitus images of all treated Triplax species. One of the rarest Central European erotyline species Combocerus glaber (Schaller) is recorded from xerothermic grasslands in North-West Poland. PMID:22140339

  7. Olfaction in the boll weevil,Anthonomus grandis Boh. (Coleoptera: Curculionidae): Electroantennogram studies.

    Science.gov (United States)

    Dickens, J C

    1984-12-01

    Electroantennogram (EAG) techniques were utilized to measure the antennal olfactory responsiveness of adult boll weevils,Anthonomus grandis Boh. (Coleoptera: Curculionidae), to 38 odorants, including both insect and host plant (Gossypium hirsutum L.) volatiles. EAGs of both sexes were indicative of at least two receptor populations: one receptor population primarily responsive to pheromone components and related compounds, the other receptor population primarily responsive to plant odors. Similar responses to male aggregation pheromone components (i.e., compounds I, II, and III + IV) were obtained from both sexes, but females were slightly more sensitive to I. Both sexes were highly responsive to components of the "green leaf volatile complex," especially the six-carbon saturated and monounsaturated primary alcohols. Heptanal was the most active aldehyde tested. More acceptors responded to oxygenated monoterpenes than to monoterpene hydrocarbons. β-Bisabolol, the major volatile of cotton, was the most active sesquiterpene. In general, males, which are responsible for host selection and pheromone production, were more sensitive to plant odors than were females. In fact, males were as sensitive to β-bisabolol and heptanal as to aggregation pheromone components. Electrophysiological data are discussed with regard to the role of insect and host plant volatiles in host selection and aggregation behavior of the boll weevil.

  8. A summary of eight traits of Coleoptera, Hemiptera, Orthoptera and Araneae, occurring in grasslands in Germany

    Science.gov (United States)

    Gossner, Martin M.; Simons, Nadja K.; Achtziger, Roland; Blick, Theo; Dorow, Wolfgang H. O.; Dziock, Frank; Köhler, Frank; Rabitsch, Wolfgang; Weisser, Wolfgang W.

    2015-03-01

    Analyses of species traits have increased our understanding of how environmental drivers such as disturbances affect the composition of arthropod communities and related processes. There are, however, few studies on which traits in the arthropod community are affected by environmental changes and which traits affect ecosystem functioning. The assembly of arthropod traits of several taxa is difficult because of the large number of species, limited availability of trait databases and differences in available traits. We sampled arthropod species data from a total of 150 managed grassland plots in three regions of Germany. These plots represent the spectrum from extensively used pastures to mown pastures to intensively managed and fertilized meadows. In this paper, we summarize information on body size, dispersal ability, feeding guild and specialization (within herbivores), feeding mode, feeding tissue (within herbivorous suckers), plant part (within herbivorous chewers), endophagous lifestyle (within herbivores), and vertical stratum use for 1,230 species of Coleoptera, Hemiptera (Heteroptera, Auchenorrhyncha), Orthoptera (Saltatoria: Ensifera, Caelifera), and Araneae, sampled by sweep-netting between 2008 and 2012. We compiled traits from various literature sources and complemented data from reliable internet sources and the authors’ experience.

  9. Host Range Specificity of Scymnus camptodromus (Coleoptera: Coccinellidae), A Predator of Hemlock Woolly Adelgid (Hemiptera: Adelgidae).

    Science.gov (United States)

    Limbu, Samita; Cassidy, Katie; Keena, Melody; Tobin, Patrick; Hoover, Kelli

    2016-02-01

    Scymnus (Neopullus) camptodromus Yu and Liu (Coleoptera: Coccinellidae) was brought to the United States from China as a potential biological control agent for hemlock woolly adelgid (Adelges tsugae Annand) (Hemiptera: Adelgidae). Scymnus camptodromus phenology is closely synchronized with that of A. tsugae and has several characteristics of a promising biological control agent. As a prerequisite to field release, S. camptodromus was evaluated for potential nontarget impacts. In host range studies, the predator was given the choice of sympatric adelgid and nonadelgid prey items. Nontarget testing showed that S. camptodromus will feed to some degree on other adelgid species, but highly prefers A. tsugae. We also evaluated larval development of S. camptodromus on pine bark adelgid (Pineus strobi (Hartig)) (Hemiptera: Adelgidae) and larch adelgid (Adelges laricis Vallot) (Hemiptera: Adelgidae); a small proportion of predator larvae was able to develop to adulthood on P. strobi or A. laricis alone. Scymnus camptodromus showed no interest in feeding on woolly alder aphid (Paraprociphilus tessellatus Fitch) (Hemiptera: Aphididae) or woolly apple aphid (Eriosoma lanigerum (Hausmann)) (Hemiptera: Aphididae), and minimal interest in cotton aphid (Aphis gossypii Glover) (Hemiptera: Aphididae) in choice and no-choice experiments. Scymnus camptodromus females did not oviposit on any host material other than A. tsugae-infested hemlock. Under the circumstances of the study, S. camptodromus appears to be a specific predator of A. tsugae, with minimal risk to nontarget species. Although the predator can develop on P. strobi, the likelihood that S. camptodromus would oviposit on pine hosts of this adelgid is small.

  10. Microstructure and nanoindentation of the rostrum of Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae)

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Sudhanshu S. [Materials Science and Engineering, Arizona State University, Tempe, AZ 85287-6106 (United States); Current address: Department of Materials Science and Engineering, Indian Institute of Technology Kanpur, Uttar Pradesh 208016 (India); Jansen, Michael A.; Franz, Nico M. [School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501 (United States); Chawla, Nikhilesh, E-mail: nchawla@asu.edu [Materials Science and Engineering, Arizona State University, Tempe, AZ 85287-6106 (United States)

    2016-08-15

    The rostrum is an extension of the cuticle of the head of weevils (Coleoptera: Curculionidae) and is often used to bore holes for oviposition (the process of laying eggs) into host plant tissue where larval development occurs. In members of the genus Curculio Linnaeus, 1758, the rostrum is long, slender, and strongly curved, but is nevertheless used to excavate straight bore-holes in the fruit of various host plants, through significant deformation of this structure. In this study, scanning electron microscopy (SEM) was used to examine the rostrum of Curculio longinasus Chittenden, 1927, leading to a microstructural model that describes its deformation behavior. Specifically, we used the continuous stiffness measurement (CSM) technique in nanoindentation to measure the Young's modulus and hardness of rostrum. The values of Young's modulus and hardness for the endocuticle were measured to be 8.91 ± 0.93 GPa and 558 ± 60 MPa, respectively. These results are critical for generating accurate finite element models of the head's mechanical behavior while it undergoes deformation. - Highlights: •SEM was used to examine the rostrum of Curculio longinasus Chittenden, 1927. •Nanoindentation to measure the Young's modulus and hardness of rostrum. •Results are critical for finite element models of the head's mechanical behavior.

  11. Population Development of Zabrotes subfasciatus (Coleoptera: Chrysomelidae) in Landrace Bean Varieties Occurring in Southwestern Amazonia.

    Science.gov (United States)

    Lopes, L M; Araújo, A E F; Santos, A C V; Santos, V B; Sousa, A H

    2016-02-01

    The common bean, Phaseolus vulgaris (L.), is one of the most important sources of protein worldwide, and Latin America is one of the recognized centers of diversity of this species. However, storage of this product after harvest is not feasible because of bruchid attacks. This study determined the accumulated normalized rate of emergence and the daily emergence rate of Zabrotes subfasciatus (Boheman) (Coleoptera: Chrysomelidae:Bruchinae) in five landrace varieties of common bean (BRL 01, SNA 01, RDR 01, RBC 01, and RBC 13) that occurin southwestern Amazonia. These varieties were selected for this study because they are well-distributed throughout the Amazonian communities. Beans of each variety were infested with 50 unsexed adults, and the insects were removed 13 d after beginning the bioassays. The adult progeny obtained from the feeding substrate were counted and removed every other day after the first emergence, until the end of the emergence period. Differences were observed in the calculated rates of development; however, the time required for development and emergence of the insects was independent. Of the five varieties of bean investigated, we observed that the RDR 01, BRL 01, and SNA 01 cultivars are resistant to Z. subfasciatus; the results indicate that the use of these three varieties can reduce problems associated with bruchid attacks and enable storage of the product after harvesting.

  12. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect

    International Nuclear Information System (INIS)

    Downey, Danielle; Chun, Stacey; Follett, Peter

    2016-01-01

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5–7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1–4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. (author)

  13. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases.

    Science.gov (United States)

    Downey, Danielle; Chun, Stacey; Follett, Peter

    2015-06-01

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  14. Susceptibility of Dalotia coriaria (Kraatz (Coleoptera: Staphylinidae to Entomopathogenic Nematodes (Rhabditida: Heterorhabditidae and Steinernematidae

    Directory of Open Access Journals (Sweden)

    Joseph Tourtois

    2015-03-01

    Full Text Available Dalotia coriaria (Kraatz (Coleoptera: Staphylinidae and entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae are two soil-dwelling biological control agents used to manage western flower thrips, Frankliniella occidentalis (Pergande (Thysanoptera: Thripidae and fungus gnats Bradysis spp. (Diptera: Sciaridae in glasshouses. Growers often use multiple natural enemies to achieve economic control, but knowledge of interactions among natural enemies is lacking. We conducted a laboratory bioassay to test the pathogenicity of four commercially available nematode species—Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhbditidae, Steinernema carpocapsae (Weiser (Rhabditida: Steinernematidae, S. feltiae (Filipjev, and S. riobrave Cabanillas et al.—to third instar and adult D. coriaria. Third instars were three times more susceptible than the adults to the entomopathogenic nematodes. Mortality for D. coriaria adults and third instars treated with S. feltiae and H. bacteriophora was lower than the mortality for D. coriaria adults and third instars treated with S. carpocapsae and S. riobrave. Neither infective juvenile foraging behavior nor size correlates with D. coriaria mortality. Dalotia coriaria appears to be most likely compatible with applications of S. feltiae and H. bacteriophora.

  15. ATIVIDADE INSETICIDA DE PLANTAS MEDICINAIS SOBRE O Callosobruchus maculatus (COLEOPTERA: BRUCHIDAE

    Directory of Open Access Journals (Sweden)

    Antonia Mirian Nogueira de Moura Guerra

    2009-01-01

    Full Text Available This study aimed to evaluate the insecticidal activity of eight medicinal plants on Callosobruchus maculatus (F. (Coleoptera: Bruchidae. Plant powder from Rosmarinus officinalis (L. leaves, Peumus boldus (Mol leaves, Matricaria chamomilla (L. flowers, Baccharis trimera (Less. leaves, Camellia sinensis (L. leaves, Thea sinensis (L. leaves, Ilex paraguariensis (St. Hil. leaves, and fruits of Pimpinella anisum (L. were used in the experiment. Bioassays were carried out under constant conditions of temperature (28±2 °C, relative humidity (70±5% and scotophase of 24 hours. Experimental units consisted of 20 grams of cowpea (Vigna unguiculata (L. Walp. grains with 13% wet basis moisture content, 2 g plant powder and ten adult insects, both placed in plastic containers (60 mm in diameter x 60 mm high. The analyzed variables included: insect mortality, oviposition (egg number and instantaneous rate of population growth (ri. The number of dead insects was recorded 5 days after bioassays were started, the egg number was recorded after 12 days, and ri was estimated on the 60th day. All the tested plants had toxicity to C. maculatus. The highest mortality was found in insects treated with P. boldus and I. paraguariensis. Number of eggs and ri also varied significantly among the powders. The lowest mean values for these parameters were recorded in the plots treated with P. boldus, P. anisum and R. officinalis.

  16. Preliminary Diversity Of Dung Beetles (Coleoptera: Scarabaeinae Attracting By Human Dung (Calvario, Meta

    Directory of Open Access Journals (Sweden)

    Ruth Mariela Castillo Morales

    2011-12-01

    Full Text Available In the Private Reserve, Refugio del Oso de Anteojos (El Calvario-Meta, the diversity of dung beetles was studied (Coleoptera: Scarabaeinae, grouped around different types of vegetation (Montane Rain Forest, Rainforest Premontane, Lower Montane Rain Forest, by using pitfall traps baited with human dung. During the samplings carried out in the rainy station on 2008, were captured a total of 177 individuals belonging to 9 species. The tribe Canthonini was the most representative with 87.5%, of the described species, followed by Aechini 10.8%, and Onthophagini 1.7%. The dominant species was Canthon lituratus in the three vegetation units: Lower montane rain forest was the richest (9 species and abundance (86 individuals, compared with other environments sampled. By calculating the complementarity index, shows a similar species composition in the three habitats sampled, possibly, related to the floristic composition presented in each one of them.

  17. Blueberry Cultivars Differ in Susceptibility to the Elephant Weevil, Orthorhinus cylindrirostris (Coleoptera: Curculionidae).

    Science.gov (United States)

    Murdoch, Gregory; Clift, Alan D; Mansfield, Sarah

    2017-10-01

    The accumulated damage from elephant weevil larvae, Orthorhinus cylindrirostris (F.) (Coleoptera: Curculionidae), reduces blueberry yield and shortens the productive lifespan of blueberry plants by several years. Selective breeding to develop pest-resistant blueberry cultivars is a possible control option, but the relationship between O. cylindrirostris populations, plant damage, and blueberry yield has not been described. A field survey of 17 blueberry cultivars was conducted on a commercial farm to measure O. cylindrirostris populations (emergence holes and adult numbers) and yield from plants of different ages (2-12 yr). Blueberry plants accumulated damage over time, that is, older plants tended to have more O. cylindrirostris emergence holes than younger plants. All cultivars received some level of O. cylindrirostris attack but this did not always lead to yield losses. Newer cultivars that have been in production since 2000 were less susceptible to O. cylindrirostris than older cultivars. Removal of highly susceptible cultivars from commercial blueberry farms may reduce O. cylindrirostris populations. There is potential for selective breeding to increase plant resistance to O. cylindrirostris if the specific resistance mechanisms can be identified in blueberry. © The Author 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  18. Coexistence and Competition between Tomicus yunnanensis and T. minor (Coleoptera: Scolytinae in Yunnan Pine

    Directory of Open Access Journals (Sweden)

    Rong Chun Lu

    2012-01-01

    Full Text Available Competition and cooperation between bark beetles, Tomicus yunnanensis Kirkendall and Faccoli and Tomicus minor (Hartig (Coleoptera: Scolytinae were examined when they coexisted together in living Yunnan pine trees (Pinus yunnanensis Franchet in Yunnan province in Southwest China. T. yunnanensis bark beetles were observed to initiate dispersal from pine shoots to trunks in November, while the majority of T. minor begins to transfer in December. T. yunnanensis mainly attacks the top and middle parts of the trunk, whereas T. minor mainly resides in the lower and middle parts of the trunk. The patterns of attack densities of these two species were similar, but with T. yunnanensis colonizing the upper section of the trunk and T. minor the lower trunk. The highest attack density of T. Yunnanensis was 297 egg galleries/m2, and the highest attack density of T. minor was 305 egg galleries/m2. Although there was significant overlap for the same bark areas, the two species generally colonize different areas of the tree, which reduces the intensity of competition for the relatively thin layer of phloem-cambium tissues where the beetles feed and reside.

  19. Visual cues are relevant in behavioral control measures for Cosmopolites sordidus (Coleoptera: Curculionidae).

    Science.gov (United States)

    Reddy, Gadi V P; Raman, A

    2011-04-01

    Trap designs for banana root borer, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae), have been done essentially on the understanding that C. sordidus rely primarily on chemical cues. Our present results indicate that these borers also rely on visual cues. Previous studies have demonstrated that among the eight differently colored traps tested in the field, brown traps were the most effective compared with the performances of yellow, red, gray, blue, black, white, and green traps; mahogany-brown was more effective than other shades of brown.In the current study, efficiency of ground traps with different colors was evaluated in the laboratory for the capture of C. sordidius. Response of C. sordidus to pheromone-baited ground traps of several different colors (used either individually or as 1:1 mixtures of two different colors) were compared with the standardized mahogany-brown traps. Traps with mahogany-brown mixed with different colors had no significant effect. In contrast, a laboratory color-choice tests indicated C. sordidus preferred black traps over other color traps, with no specific preferences for different shades of black. Here again, traps with black mixed with other colors (1:1) had no influence on the catches. Therefore, any other color that mixes with mahogany-brown or black does not cause color-specific dilution of attractiveness. By exploiting these results, it may be possible to produce efficacious trapping systems that could be used in a behavioral approach to banana root borer control.

  20. Attraction of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) to four varieties of Lathyrus sativus L. seed volatiles.

    Science.gov (United States)

    Adhikary, P; Mukherjee, A; Barik, A

    2015-04-01

    Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) is an important stored grain pest of Lathyrus sativus L. (Leguminosae), commonly known as khesari, in India, Bangladesh and Ethiopia. Volatiles were collected from four varieties, i.e., Bio L 212 Ratan, Nirmal B-1, WBK-14-7 and WBK-13-1 of uninfested khesari seeds, and subsequently identified and quantified by gas chromatography mass spectrometry and gas chromatography flame ionization detector analyses, respectively. A total of 23 volatiles were identified in the four varieties of khesari seeds. In Bio L 212 Ratan and WBK-13-1 seeds, nonanal was the most abundant followed by farnesyl acetone; whereas farnesyl acetone was predominant followed by nonanal in Nirmal B-1 and WBK-14-7 khesari seeds. The olfactory responses of female C. maculatus toward volatile blends from four varieties of khesari seeds, and individual synthetic compounds and their combinations were examined through Y-shaped glass tube olfactometer bioassays. Callosobruchus maculatus showed significant preference for the whole volatile blends from Bio L 212 Ratan seeds compared to whole volatile blends from other three varieties. The insect exhibited attraction to five individual synthetic compounds, 3-octanone, 3-octanol, linalool oxide, 1-octanol and nonanal. A synthetic blend of 448, 390, 1182, 659 and 8114 ng/20 μl methylene chloride of 3-octanone, 3-octanol, linalool oxide, 1-octanol and nonanal, respectively, was most attractive to C. maculatus, and this combination might be used for insect pest management program such as baited traps.

  1. Aktivitas minyak dan serbuk enam spesies tumbuhan terhadap peneluran dan Mortalitas Callosobruchus sp. (Coleoptera: Bruchidae

    Directory of Open Access Journals (Sweden)

    Dadang Dadang

    2017-02-01

    Full Text Available Storage pests remain one of the main problems in storage. The pest attacks occur not only in the field, but also in storage. Control should be done to maintain the quality of storedproducts. The environmentally friendly measures should be implemented to avoid negative impacts to the environment and human being. The objective of this research was to study the mortality and oviposition deterrence caused by powder and oil of six plant species against Callosobruchus sp. (Coleoptera: Bruchidae. Flowers of Eugenia aromatica, roots of Vetiveria zizanioides, leaves of Pogostemon cablin, seeds of Ricinus communis, seeds of Foeniculum vulgare, and stems of Cymbopogon citratus were air-dried and milled to yield powder. Oil of six plant species were purchased from local market. Oviposition deterrent bioassay was conducted by no-choice method for both powder and oil, while mortality bioassay was conducted by topical application and residual methods for plant oil only. Several plant species showed high biological activity to Callosobruchus sp. Powder of E. aromatica and oil of V. zizanioides caused high oviposition deterrence, while oil of V. zizanioides and E. aromatica caused high mortality.

  2. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae

    Directory of Open Access Journals (Sweden)

    Andréa Roveré Franz

    2011-03-01

    Full Text Available Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae. Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf, Zingiber officinale (root and Mentha sp. (leaf. The efficacy of these oils was tested to control the rice weevil, S. oryzae, using hydrodistillation. Chemical analysis of the essential oils was carried out by gas chromatography. Major components of C. citratus were geranial (48% and neral (31%, of Z. officinale were α-zingibereno (13%, geranial (16%, neral (10% and α-farneseno (5% and of Mentha sp. was menthol (92%. Bioassays were carried out by fumigation and topical application. In topical application assays, the essential oil of C. citratus had greater toxicity (LC50 0.027 µL mL-1 and shorter exposure time than the oils of the other two plants. After 24 h and 48 h, 70% and 100% mortality of S. oryzae occurred, respectively. In fumigation assays, essential oil of Z. officinale had a lower LC50 (1.18 µL cm-2 and 70% mortality after 24 h exposure. Therefore, we recommend the use of essential oils of C. citratus and Z. officinale to control the rice weevil S. oryzae.

  3. The aquatic Coleoptera of Prince Edward Island, Canada: new records and faunal composition

    Directory of Open Access Journals (Sweden)

    Christopher Majka

    2008-09-01

    Full Text Available The aquatic Coleoptera (Gyrinidae, Haliplidae, Dytiscidae, Hydrophilidae, Elmidae, Dryopidae, Heteroceridae of Prince Edward Island, Canada is surveyed. Seventy-two species are now known to occur on Prince Edward Island, 26 of which are added to the island's faunal list. Three species, Gyrinus aquiris LeConte, Oulimnius latiusculus (LeConte, and Helichus striatus LeConte, are removed since there are no voucher specimens or published records to substantiate their presence. The name Dineutus horni is designated as an incorrect subsequent spelling of Dineutus hornii Roberts, 1895. The composition of the fauna is briefly discussed, both from regional and zoogeographic perspectives. There is only one introduced species, Helophorus grandis Illiger. Only one third of the aquatic fauna recorded on the neighbouring mainland has been found on Prince Edward Island, perhaps reflecting an island-associated diminution, the paucity of collecting, an area effect, or a combination of all these factors. The island faunas of Prince Edward Island, Cape Breton Island, and insular Newfoundland are compared. Prince Edward Island's is 36% smaller than the others, in contrast with the island's carabid fauna which is almost identical in magnitude with that of Cape Breton. This might reflect dispersal obstacles, the relative paucity of aquatic habitats on the island, or an insufficient collecting effort. Further research would be desirable, both to better discern the composition of the province's fauna, as well as to monitor the health of aquatic ecosystems in relation to anthropogenic activities.

  4. Observations on the Cave-Associated Beetles (Coleoptera of Nova Scotia, Canada

    Directory of Open Access Journals (Sweden)

    Moseley M.

    2009-07-01

    Full Text Available The cave-associated invertebrates of Nova Scotia constitute a fauna at a very early stage of post-glacial recolonization. TheColeoptera are characterized by low species diversity. A staphylinid Quedius spelaeus spelaeus, a predator, is the only regularlyencountered beetle. Ten other terrestrial species registered from cave environments in the province are collected infrequently. Theyinclude three other rove-beetles: Brathinus nitidus, Gennadota canadensis and Atheta annexa. The latter two together with Catopsgratiosus (Leiodidae constitute a small group of cave-associated beetles found in decompositional situations. Quedius s. spelaeusand a small suite of other guanophiles live in accumulations of porcupine dung: Agolinus leopardus (Scarabaeidae, Corticariaserrata (Latrididae, and Acrotrichis castanea (Ptilidae. Two adventive weevils Otiorhynchus ligneus and Barypeithes pellucidus(Curculionidae collected in shallow cave passages are seasonal transients; Dermestes lardarius (Dermestidae, recorded fromone cave, was probably an accidental (stray. Five of the terrestrial beetles are adventive Palaearctic species. Aquatic beetles arecollected infrequently. Four taxa have been recorded: Agabus larsoni (Dytiscidae may be habitual in regional caves; another Agabussp. (probably semivittatus, Dytiscus sp. (Dytiscidae, and Crenitis digesta (Hydrophilidae are accidentals. The distribution andecology of recorded species are discussed, and attention is drawn to the association of beetles found in a Nova Scotia “ice cave”.

  5. Description of immatures and mating behavior of Liogenys bidenticeps Moser, 1919 (Coleoptera: Melolonthidae: Melolonthinae

    Directory of Open Access Journals (Sweden)

    Sérgio Roberto Rodrigues

    Full Text Available Abstract Description of immatures and mating behavior of Liogenys bidenticeps Moser, 1919 (Coleoptera: Melolonthidae: Melolonthinae. Some species of Melolonthinae are associated with several species of cropped plants, with larvae consuming roots and, in some cases, are considered as crop pests. In some agricultural regions of Brazil, larvae of L. bidenticeps are found associated with cultivated plants, and little information is available about this taxon. This study, aiming at expanding the knowledge about the morphology and behavior of this species, provides the description of immatures and mating behavior of adults. The studies were conducted at the experimental farm of the Universidade Estadual de Mato Grosso do Sul in Aquidauana, Mato Grosso do Sul state, Brazil, and the adults were collected with light trap and raised in the laboratory. Mating behavior was documented on video both in the field and under laboratory conditions. Descriptions and illustrations of the third instar larva and pupa are presented. Adults have crepuscular flight activity and their copulation lasts an average of 20.25 min, occurring from 19:00 to 22:00 h. On some occasions, females did not accept males for copulation, indicating an active selection of males by females. Field observations demonstrated that adults feed on Brazilian pepper leaves (Schinus terebinthifolius, Anacardiaceae and cashew flowers (Anacardium occidentale, Anacardiaceae, where male and female meet each other and copulation occurs.

  6. Soil management system in hazelnut groves (Corylus sp. versus the presence of ground beetles (Coleoptera: Carabidae

    Directory of Open Access Journals (Sweden)

    Nietupski Mariusz

    2015-01-01

    Full Text Available Sustaining biodiversity as well as taking advantage of the natural environment’s resistance are the key elements which should be considered when designing integrated plans for the protection of hazelnut groves. An effort has been made in this study to analyse the impact of different soil cultivation methods in hazelnut groves, on the species composition and number of individuals in carabid assemblages (Coleoptera: Carabidae. Another aim was to determine which method of inter-row soil management had the least negative effect on assemblages of these beetles. Because of the type of habitat, the xerothermic species characteristic for southeastern Europe, i.e. Calathus ambiguus, Poecilus lepidus, Harpalus calceatus, and H. griseus, were the most numerous. The qualitative and quantitative analysis of the captured individuals implied that the optimal soil tillage system in young hazelnut groves is when soil is kept fallow with machines or chemicals, or when soil is covered with manure. The least favourable practice for the appearance of ground beetles of the Carabidae family is the use of polypropylene fabric, bark or sawdust, to cover soil

  7. A molecular phylogeny shows the single origin of the Pyrenean subterranean Trechini ground beetles (Coleoptera: Carabidae).

    Science.gov (United States)

    Faille, A; Ribera, I; Deharveng, L; Bourdeau, C; Garnery, L; Quéinnec, E; Deuve, T

    2010-01-01

    Trechini ground beetles include some of the most spectacular radiations of cave and endogean Coleoptera, but the origin of the subterranean taxa and their typical morphological adaptations (loss of eyes and wings, depigmentation, elongation of body and appendages) have never been studied in a formal phylogenetic framework. We provide here a molecular phylogeny of the Pyrenean subterranean Trechini based on a combination of mitochondrial (cox1, cyb, rrnL, tRNA-Leu, nad1) and nuclear (SSU, LSU) markers of 102 specimens of 90 species. We found all Pyrenean highly modified subterranean taxa to be monophyletic, to the exclusion of all epigean and all subterranean species from other geographical areas (Cantabrian and Iberian mountains, Alps). Within the Pyrenean subterranean clade the three genera (Geotrechus, Aphaenops and Hydraphaenops) were polyphyletic, indicating multiple origins of their special adaptations to different ways of life (endogean, troglobitic or living in deep fissures). Diversification followed a geographical pattern, with two main clades in the western and central-eastern Pyrenees respectively, and several smaller lineages of more restricted range. Based on a Bayesian relaxed-clock approach, and using as an approximation a standard mitochondrial mutation rate of 2.3% MY, we estimate the origin of the subterranean clade at ca. 10 MY. Cladogenetic events in the Pliocene and Pleistocene were almost exclusively within the same geographical area and involving species of the same morphological type.

  8. Ground beetles (Coleoptera, Carabidae of the Hanford Nuclear Site in south-central Washington State

    Directory of Open Access Journals (Sweden)

    Chris Looney

    2014-04-01

    Full Text Available In this paper we report on ground beetles (Coleoptera: Carabidae collected from the Hanford Nuclear Reservation and Hanford National Monument (together the Hanford Site, which is located in south-central Washington State. The Site is a relatively undisturbed relict of the shrub-steppe habitat present throughout much of the western Columbia Basin before the westward expansion of the United States. Species, localities, months of capture, and capture method are reported for field work conducted between 1994 and 2002. Most species were collected using pitfall traps, although other capture methods were employed. Trapping results indicate the Hanford Site supports a diverse ground beetle community, with over 90% of the 92 species captured native to North America. Four species collected during the study period are newly recorded for Washington State: Bembidion diligens Casey, Calosoma obsoletum Say, Pseudaptinus rufulus (LeConte, and Stenolophus lineola (Fabricius. Based on these data, the Site maintains a diverse ground beetle fauna and, due to its size and diversity of habitats, is an important repository of shrub-steppe biodiversity.

  9. A comparison of ground beetle assemblages (Coleoptera: Carabidae in conventionally and ecologically managed alfalfa fields

    Directory of Open Access Journals (Sweden)

    P. Kolařík

    2014-12-01

    Full Text Available From 2007-2011, the occurrence of ground beetles (Coleoptera: Carabidae was studied using emergence traps in two differently managed alfalfa fields in the Czech Republic - a conventional and an ecological production system. In total, 784 specimens of ground beetles representing 58 species were trapped in these two alfalfa fields in South Moravia. A slightly higher number of specimens were trapped in the conventionally managed than in the ecological alfalfa stand (404 vs 380, respectively. In the conventionally managed alfalfa stand, the number of species was also higher than in the ecological stand (45 vs 40, respectively. With the exception of 2007 and 2009, Simpson’s indices of diversity were higher in the conventional stand than in the ecological in all study years. Shannon’s index was higher in the conventional alfalfa field in 2008, 2009, and 2011. Regarding distribution, species classified into group E (i.e., those without special demands on the type and quality of their habitat dominated in both types of management throughout the experimental period. The incidence of species classified into group R (i.e., those with narrow ecological amplitude was very low; i.e., only four species. These ground beetle species are included in the Red List of Threatened Species of the Czech Republic, and all of them (i.e. Acupalpus suturalis, Calosoma auropunctatum, Cicindela germanica and Ophonus cribricollis are listed as vulnerable.

  10. Effects of insecticide exposure on movement and population size estimates of predatory ground beetles (Coleoptera: Carabidae).

    Science.gov (United States)

    Prasifka, Jarrad R; Lopez, Miriam D; Hellmich, Richard L; Prasifka, Patricia L

    2008-01-01

    Estimates of arthropod population size may paradoxically increase following insecticide applications. Research with ground beetles (Coleoptera: Carabidae) suggests that such unusual results reflect increased arthropod movement and capture in traps rather than real changes in population size. However, it is unclear whether direct (hyperactivity) or indirect (prey-mediated) mechanisms produce increased movement. Video tracking of Scarites quadriceps Chaudior indicated that brief exposure to lambda-cyhalothrin or tefluthrin increased total distance moved, maximum velocity and percentage of time moving. Repeated measurements on individual beetles indicated that movement decreased 240 min after initial lambda-cyhalothrin exposure, but increased again following a second exposure, suggesting hyperactivity could lead to increased trap captures in the field. Two field experiments in which ground beetles were collected after lambda-cyhalothrin or permethrin application attempted to detect increases in population size estimates as a result of hyperactivity. Field trials used mark-release-recapture methods in small plots and natural carabid populations in larger plots, but found no significant short-term (<6 day) increases in beetle trap captures. The disagreement between laboratory and field results suggests mechanisms other than hyperactivity may better explain unusual changes in population size estimates. When traps are used as a primary sampling tool, unexpected population-level effects should be interpreted carefully or with additional data less influenced by arthropod activity.

  11. Seed Detection and Discrimination by Ground Beetles (Coleoptera: Carabidae) Are Associated with Olfactory Cues.

    Science.gov (United States)

    Kulkarni, Sharavari S; Dosdall, Lloyd M; Spence, John R; Willenborg, Christian J

    2017-01-01

    Olfactory ability is an element of fitness in many animals, guiding choices among alternatives such as mating partners or food. Ground beetles (Coleoptera; Carabidae), exhibit preferences for prey, and some species are well-known weed seed predators. We used olfactometer-based bioassays to determine if olfactory stimuli are associated with detection of Brassica napus L., Sinapis arvensis L., and Thlaspi arvense L. seeds by ground beetles characteristic of agroecosystems, and whether behavioural responses to seed odors depended on seed physiological state (imbibed or unimbibed). Imbibed B.napus seeds were preferred over other weed species by two of the three carabid species tested. Only A. littoralis responded significantly to unimbibed seeds of B. napus. Sensitivity to olfactory cues appeared to be highly specific as all carabid species discriminated between the olfactory cues of imbibed brassicaceous weed seeds, but did not discriminate between weed seeds that were unimbibed. Overall, our data suggest that depending on seed physiological state, odours can play an important role in the ability of carabids to find and recognize seeds of particular weed species.

  12. Development and Life History of Sitophilus zeamais (Coleoptera: Curculionidae on Cereal Crops

    Directory of Open Access Journals (Sweden)

    James Adebayo Ojo

    2016-01-01

    Full Text Available The maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae, is one of the most destructive pests of stored cereals. Knowledge of the life history and biology is important to the development of an integrated pest management program. Investigation was carried out on developmental biology of S. zeamais on four main cereal crops, maize, rice, sorghum, and millet, under laboratory conditions. Egg incubation, oviposition periods, and larval instar development were not different significantly among the food hosts. Number of eggs laid varied significantly among the cereal grains; mean fecundity was highest on maize (67.2±3.16 and lowest on millet (53.8±0.17. Number of immature (larva and pupa and adult stages varied significantly among the cereal grains. There exist four larval instars with a varied mean head capsule width, with a mean total instar larval developmental period of 23.1, 22.2, 22.2, and 21.6 d on maize, rice, sorghum, and millet, respectively. There was linear relationship and significant correlation between the stages of larval development and head capsule width. The mean developmental period from egg to adult varied, being highest on maize (34.7 d and lowest on sorghum (33.5 d.

  13. Males of Hylamorpha elegans burmeister (Coleoptera: Scarabaeidae) are attracted to odors released from conspecific females.

    Science.gov (United States)

    Quiroz, Andrés; Palma, Ruben; Etcheverría, Paulina; Navarro, Vicente; Rebolledo, Ramón

    2007-04-01

    The behavioral responses of Hylamorpha elegans L. (Coleoptera: Scarabaeidae, Rutelinae) to the semiochemicals released from conspecific individual adults were studied, with particular attention paid to female attraction of males. Odors released from virgin females significantly attracted male conspecifics in both the field and laboratory olfactometer and wind tunnel bioassays. However, females did not attract other females, and males attracted no one. The response of male H. elegans to (1) compounds (1,4-hydroquinone and 1,4-benzoquinone) released only by unmated females; (2) the essential oil of the secondary host (Nothofagus obliqua); and (3) the blend of 1,4-hydroquinone and 1,4-benzoquinone with N. obliqua essential oil was studied. The blend of 1,4-benzoquinone mixed with essential oil at the trial concentration was attractive with males. The same response was found with 1,4-hydroquinone alone. The essential oil did not have the expected attractant effect on conspecific males. These results suggest that, when combined with essential oil, 1,4-benzoquinone may function in the sexual behavior of males and females. These findings are discussed in terms of the ecological role of this putative sexual pheromone and its potential use in a strategy of control of this pest.

  14. Notas e novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2007-01-01

    Full Text Available Novas espécies descritas do Brasil: Hypsioma carioca sp. nov. (Rio de Janeiro e Hesychotypa maraba sp. nov. (Pará; do Equador: Sternycha ecuatoriana sp. nov. (Pichincha; da Bolívia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. e Alexera secunda sp. nov. Transfere-se Hesycha strandi (Breuning, 1943 para o gênero Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 é considerada sinônima de H. miniata Thomson, 1868. Novo registro (Trinidad e figura são dados para Trachysomus surdus.Notes and new species of Onciderini (Coleoptera, Cerambycidae, Lamiinae. New species described from Brazil: Hypsioma carioca sp. nov. (Rio de Janeiro and Hesychotypa maraba sp. nov. (Pará; from Ecuador: Sternycha ecuatoriana sp. nov. (Pichincha; from Bolivia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. and Alexera secunda sp. nov. Hesycha strandi (Breuning, 1943 is transferred to the genus Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 is considered a synonym of H. miniata Thomson, 1868. New record (Trinidad and figure for Trachysomus surdus Dillon & Dillon, 1946 are given.

  15. Trapping Phyllophaga spp. (Coleoptera: Scarabaeidae: Melolonthinae) in the United States and Canada using sex attractants.

    Science.gov (United States)

    Robbins, Paul S.; Alm, Steven R.; Armstrong, Charles. D.; Averill, Anne L.; Baker, Thomas C.; Bauernfiend, Robert J.; Baxendale, Frederick P.; Braman, S. Kris; Brandenburg, Rick L.; Cash, Daniel B.; Couch, Gary J.; Cowles, Richard S.; Crocker, Robert L.; DeLamar, Zandra D.; Dittl, Timothy G.; Fitzpatrick, Sheila M.; Flanders, Kathy L.; Forgatsch, Tom; Gibb, Timothy J.; Gill, Bruce D.; Gilrein, Daniel O.; Gorsuch, Clyde S.; Hammond, Abner M.; Hastings, Patricia D.; Held, David W.; Heller, Paul R.; Hiskes, Rose T.; Holliman, James L.; Hudson, William G.; Klein, Michael G.; Krischik, Vera L.; Lee, David J.; Linn, Charles E.; Luce, Nancy J.; MacKenzie, Kenna E.; Mannion, Catherine M.; Polavarapu, Sridhar; Potter, Daniel A.; Roelofs, Wendell L.; Royals, Brian M.; Salsbury, Glenn A.; Schiff, Nathan M.; Shetlar, David J.; Skinner, Margaret; Sparks, Beverly L.; Sutschek, Jessica A.; Sutschek, Timothy P.; Swier, Stanley R.; Sylvia, Martha M.; Vickers, Neil J.; Vittum, Patricia J.; Weidman, Richard; Weber, Donald C.; Williamson, R. Chris; Villani, Michael G

    2006-01-01

    The sex pheromone of the scarab beetle, Phyllophaga anxia, is a blend of the methyl esters of two amino acids, L-valine and L-isoleucine. A field trapping study was conducted, deploying different blends of the two compounds at 59 locations in the United States and Canada. More than 57,000 males of 61 Phyllophaga species (Coleoptera: Scarabaeidae: Melolonthinae) were captured and identified. Three major findings included: (1) widespread use of the two compounds [of the 147 Phyllophaga (sensu stricto) species found in the United States and Canada, males of nearly 40% were captured]; (2) in most species intraspecific male response to the pheromone blends was stable between years and over geography; and (3) an unusual pheromone polymorphism was described from P. anxia. Populations at some locations were captured with L-valine methyl ester alone, whereas populations at other locations were captured with L-isoleucine methyl ester alone. At additional locations, the L-valine methyl ester-responding populations and the L-isoleucine methyl ester-responding populations were both present, producing a bimodal capture curve. In southeastern Massachusetts and in Rhode Island, in the United States, P. anxia males were captured with blends of L-valine methyl ester and L-isoleucine methyl ester. PMID:19537965

  16. ECOLOGICAL AND FAUNISTIC REVIEW OF COCCINELLIDAE (COLEOPTERA, COCCINELLIDAE IN THE REPUBLIC OF DAGESTAN

    Directory of Open Access Journals (Sweden)

    G. M. Mukhtarova

    2016-01-01

    Full Text Available Aim. The aim of the study is to conduct ecological and faunal studies of coccinellidae in Dagestan as well as to consider the chorologic, trophic and phenological aspects in connection with the peculiarities of the environment.Materials and methods. As the basis for the research we used the materials obtained by the authors from 1999 to 2016 in various parts of Dagestan. We also conducted expeditions, stationary and semi-stationary studies using traditional methods of entomological research.Results. Inventory of coccinellidae fauna in Dagestan allowed identifying 27 species from 18 genera. It also revealed multiple and rare species. We carried out an analysis on distribution of the species in the high altitude range; identified environmental groups by biotopic preferendum: xerophyllous, mesophyll meadow-steppe, mesophyll forest, and Hygrophila and polytopic species; according to trophic adaptation: Aphidophages, Coccidophages, Mycetophages, Entomophages and Phytophages; We held a zoogeographical analysis of coccinellidae of Dagestan and according to habitat type, 8 zoogeographic groups were identified; We conducted phenological observations of the study sites, stages and timing of winter and winter dormancy, the beginning of mating, egg-laying, the duration of certain stages, the number of generations and other features.Conclusion. This research represents the first comprehensive summary of ladybugs (Coleoptera, Coccinellidae of Dagestan and includes the findings of the composition study, ecological and zoogeographical aspects of coccinellidae fauna of the study area.

  17. Resinosis Inhibits Monochamus spp. (Coleoptera: Cerambycidae) Colonization of Healthy Shortleaf Pines in Southeastern United States.

    Science.gov (United States)

    Ethington, Matthew W; Galligan, Larry D; Stephen, Fred M

    2018-05-14

    The genus Monochamus Dejean (Coleoptera: Cerambycidae) includes large, woodboring, longhorned beetles, which colonize pine trees in North America. Many authors have classified the genus as saprophagous, but one recent study reported successful colonization of standing jack pine trees (Pinus banksiana Lamb.) (Pinales: Pinaceae) following severe wind disturbance in Minnesota. We tested whether two Monochamus species native to the southeastern United States (M. titillator (Fabricius) and M. carolinensis (Olivier)) could successfully colonize healthy shortleaf pines (Pinus echinata Mill.) (Pinales: Pinaceae) in recently harvested stands without coincident abiotic or biotic stressors, such as lightning strikes or bark beetle attacks. We attached commercially available semiochemical lures, including monochamol, ethanol, and ipsenol, to healthy shortleaf pine trees and observed Monochamus spp. oviposition response. Egg development was monitored following oviposition by harvesting attacked trees and dissecting oviposition pits. High numbers of oviposition pits were observed on trees treated with lures containing the bark beetle pheromone ipsenol and pits were highly concentrated on the tree bole near lures. Although egg deposition occurred, pit dissection revealed large amounts of resin present in almost all dissected pits and that egg hatch and subsequent larval development were rare. Our results demonstrate that southeastern Monochamus spp. are unlikely to be primary pests of healthy shortleaf pines due to resinosis. To better understand the host finding behavior of these two Monochamus species, we also conducted trapping trials with several semiochemical combinations. Both species and sexes demonstrated similar attraction to compounds, and the most attractive lure combined host volatiles, pheromone, and sympatric insect kairomone.

  18. Revision of the genus Ptomaphagus Hellwig from eastern Asia (Coleoptera, Leiodidae, Cholevinae).

    Science.gov (United States)

    Wang, Cheng-Bin; Perreau, Michel; Růžička, Jan; Nishikawa, Masaaki

    2017-01-01

    The species belonging to the genus Ptomaphagus Hellwig, 1795 (Coleoptera, Leiodidae, Cholevinae, Ptomaphagini) from eastern Asia are assigned to three species groups. Group yasutoshii has a single species: P. (s. str.) yasutoshii Nishikawa, 1993 from Taiwan, China. Group nepalensis with three species: P. (s. str.) nepalensis Perreau, 1988 from Nepal and P. (s. str.) masumotoi Nishikawa, 2011 from Thailand are redescribed, and P. (s. str.) piccoloi Wang, Růžička, Nishikawa, Perreau & Hayashi, 2016 is recorded for the first time from China (Zhejiang). Group sibiricus with seven species, including two newly described Chinese ones P. (s. str.) funiu sp. n. from Henan, and P. (s. str.) haba sp. n. from Yunnan, and five known species: P. (s. str.) chenggongi Wang, Nishikawa, Perreau, Růžička & Hayashi, 2016, P. (s. str.) hayashii Wang, Růžička, Perreau, Nishikawa & Park, 2016, P. (s. str.) kuntzeni Sokolowski, 1957 (distribution records from Myanmar excluded), P. (s. str.) sibiricus Jeannel, 1934 and P. (s. str.) tingtingtae Wang, Nishikawa, Perreau, Růžička & Hayashi, 2016. Specimens of other undescribed species of the group sibiricus are also recorded, revealing a high diversity of this genus in eastern Asia, especially in central and north Sichuan, China, which essentially remains to be investigated. Relevant morphological characters of the examined species are illustrated with colour plates, and their known distributions are mapped. A key to species of Ptomaphagus from eastern Asia is provided.

  19. BIOECOLOGY AND MANAGEMENT OF ERVA-MATE BORER, Hedypathes betulinus (KLUG, 1825 (COLEOPTERA: CERAMBYCIDAE

    Directory of Open Access Journals (Sweden)

    Márcia d´Avila

    2010-08-01

    Full Text Available The erva-mate (Ilex paraguariensis A.St.-Hil. has a social, cultural and economic importance in  the southern states of Brazil. The  pure stands of  this culture was responsible for the increase  of many species of insects. Hedypathes betulinus (Klug, 1825 (Coleoptera: Cerambycidae is considered the main pest from an economic viewpoint because of its difficult control and potential for damage. The larval phase occurs inside the twings and trunks, what makes more difficult to deal with its detention and management. Cultural and mechanical management are the most indicated, such as collection of adults, prunning and burning of plant parts damaged by the insect, balanced nutrition, adequate plant density and maintenance of areas with native vegetation or also the introduction of policulture. These strategies  may increase  the  agroecossystem  balance  and  thus  a  reduction  of  the  insect-pest  to  an aceptable level. Therefore, the objective of this study was to assemble and the discuss the information on the bioecology and management of erva-mate borer.

  20. Caracteres preimaginales y aspectos bionómicos de Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Jerez, V.

    2011-12-01

    Full Text Available We describe the morphology and microstructure of the egg chorion, and the morphology of the first instar larva of Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae, Nycteliini. Bionomical and distributional data on this species are also provided. To obtain eggs and larvae, couples were collected in the field and kept in rearing cages until oviposture and ecclosion. The structure and adornment of the egg exochorion, and the exterior morphological features of larvae were examined with electron scanning microscopy. The eggs of G. luczotii showed a rounded micropyle and a smooth exochorion, composed of hexagonal cells without aeropyles. The larvae of G. luczotii showed morphological characteristics suited for an edaphic life similar to that of Pedobionta: digging prothoracic legs, cephalic capsule with abundant sensilla, and well–developed pygopodium. We analysed the importance of larval morphology as an element for specific diagnosis and found that interspecific differences regarding frontal sensilla, clypeus shape, and anterior part of labrum, had a taxonomic value and possibly a phylogenetic value.

  1. Naturally-Occurring Entomopathogenic Fungi on Three Bark Beetle Species (Coleoptera: Curculionidae in Bulgaria

    Directory of Open Access Journals (Sweden)

    Slavimira A. Draganova

    2010-01-01

    Full Text Available Bark beetles (Coleoptera: Curculionidae: Scolytinae belong to one of the most damaging groups of forest insects and the activity of their natural enemies –pathogens, parasitoids,parasites or predators suppressing their population density,is of great importance. Biodiversity of entomopathogenic fungi on bark beetles in Bulgaria has been investigated sporadically. The aim of this preliminary study was to find, identify and study morphological characteristics of fungal entomopathogens naturally-occurring in populations of three curculionid species – Ips sexdentatus Boern, Ips typographus (L. and Dryocoetes autographus (Ratz.. Dead pest adults were found under the bark of Pinus sylvestris and Picea abies trees collectedfrom forests in the Maleshevska and Vitosha Mountains. Fungal pathogens were isolated into pure cultures on SDAY (Sabouraud dextrose agar with yeast extract and were identified based on morphological characteristics both on the host and in a culture.Morphological characteristics of the isolates were studied by phenotypic methods. The fungal isolates obtained from dead adults of Ips sexdentatus, Ips typographus and D. autographus were found to belong to the species Beauveria bassiana (Bals. – Criv. Vuillemin,Beauveria brongniartii (Saccardo Petch and Isaria farinosa (Holmsk. Fries (anamorph Ascomycota, Sordariomycetes: Hypocreales, Cordycipitaceae. Morphological traits of the isolates are described.

  2. ATIVIDADE INSETICIDA DE ÓLEOS VEGETAIS SOBRE Sitophilus zeamais MOTS. (COLEOPTERA: CURCULIONIDAE EM MILHO ARMAZENADO

    Directory of Open Access Journals (Sweden)

    Rodrigo Leandro Braga de Castro Coitinho

    2006-01-01

    Full Text Available Oils of andiroba (Carapa guianensis Aubl., copaiba (Copaifera sp., Eucaliptus globulus Labill. and Eucaliptus citriodora Hook., neem (Azadirachta indica A. Juss, eugenol, souari nut (Caryocar brasiliense Camb., rosemary (Lippia gracillis HBK., and cedar (Cedrela fissilis Vell. were evaluated in adults of Sitophilus zeamais Mots. (Coleoptera: Curculionidae. In the non-choice test, 20 g of corn grains per plot were treated with 50¿L of each oil and homogenized for two minutes. The plot was infested with eight 0 to 15 days-old S. zeamais adults. The E. globulus, eugenol, rosemary, and neem oils caused 100% mortality in S. zeamais adults. Except for copaiba, all oils caused mortality above 87% and the reduction in emergence of 100%. In the free-choice tests, arenas consisting of two plastic containers symmetrically interconnected to a central box by two plastic tubes were used. Twenty grams of non-treated corn (control were placed in one of the boxes and the same amount of grain treated with 50¿L of each oil comprised the other treatment. Sixteen non-sexed S. zeamais adults (0-15 days old were released in the central box. The rosemary, E. citriodora, eugenol and copaiba oils were the most repellent for S. zeamais adults, with repellence percentage varying from 97.3 to 87.7. Rosemary, andiroba, neem, and cedar reduced S. zeamais emergence in 100%, while the reduction caused by the other oils ranged from 72.7 to 97.9%.

  3. The European lesser glow worm, Phosphaenus hemipterus (Goeze, in North America (Coleoptera, Lampyridae

    Directory of Open Access Journals (Sweden)

    Christopher Majka

    2009-12-01

    Full Text Available Phosphaenus hemipterus (Goeze is a Palaearctic glow worm (Coleoptera: Lampyridae, previously been reported in North America on the basis of two specimens; one collected in 1947 in Yarmouth, Nova Scotia; the other in 1989 in Montreal, Quebec. The present study newly records it from three sites in Halifax, Nova Scotia. One hundred and twenty six adult males and larvae were collected in 2009 in disturbed urban grassland areas, similar to habitats in England and Belgium where the species has been investigated. Experiments confirm that larvae feed on earthworms (Lumbricus terrestris, consistent with observations in Europe. The habitat is described, including vegetation, potential predators, and prey. Although ballast-shipments have previously been proposed as a vector for the species’ introduction to North America, the present study suggests that the importation of agricultural and horticultural products, which has lead to the introduction of many earthworms to the continent, could also serve as a conduit for the introduction of obligate earthworm predators such as the larvae of P. hemipterus. Although an adventive species, possible conservation concerns are discussed for a species that is considered endangered in parts of its native range.

  4. Danos de Conotrachelus dubiae (Coleoptera: curculionidae em frutos de camu-camu (Myrciaria dubia na Amazônia Central Damage of camu-camu (Myrciaria dubia fruits by Conotrachelus dubiae (Coleoptera: curculionidae in Central Amazonia

    Directory of Open Access Journals (Sweden)

    Sidney Alberto do Nascimento Ferreira

    2003-12-01

    Full Text Available No Brasil, a ocorrência de Conotrachelus dubiae O'Brien, 1995 (Coleoptera: Curculionidae em camu-camu [Myrciaria dubia (H.B.K. McVaugh, Myrtaceae] tinha sido constatada somente em populações naturais. Relata-se sua ocorrência em um cultivo experimental, onde se avaliou os danos de C. dubiae em frutos de camu-camu, em diferentes graus de amadurecimento, entre 1999 e 2003. Os danos causados pela larva aumentaram com o amadurecimento dos frutos, havendo maior comprometimento da polpa do fruto (30 a 90% do que das sementes (7%. A incidência desse inseto pode implicar em perdas quantitativas significativas na produção de camu-camu.In Brazil, the occurrence of Conotrachelus dubiae O'Brien, 1995 (Coleoptera: Curculionidae in camu-camu [Myrciaria dubia (H.B.K. McVaugh, Myrtaceae] had only been verified in natural populations. This report describes its occurrence in an experimental cultivation, where damage of camu-camu fruits by C. dubiae at different ripening stages was evaluated between 1999 and 2003. The damage caused by the larva increased with the degree of ripening of the fruits, with greater damage of fruit pulp (30 to 90% than to seeds (7%. The incidence of this insect may cause significant quantitative losses in the camu-camu production.

  5. Los coleópteros y heterópteros acuáticos del Parque Nacional Calilegua (Provincia de Jujuy, Argentina Aquatic Coleoptera and Heteroptera from Calilegua National Park (Jujuy Province, Argentina

    Directory of Open Access Journals (Sweden)

    Patricia L. M. Torres

    2008-06-01

    Full Text Available Se expone una lista de las especies de Coleoptera y Heteroptera presentes en los cuerpos de agua del Parque Nacional Calilegua. Se registraron 51 especies de Coleoptera incluidas en 28 géneros y cinco familias, y 37 especies de Heteroptera distribuidas en 22 géneros y 10 familias; sólo cuatro especies de Coleoptera y cinco de Heteroptera fueron citadas previamente del parque. Se mencionan por primera vez para la Argentina dos especies de Veliidae (Heteroptera: Rhagovelia trista Gould y Platyvelia brachialis (Stål, así como una especie y un género de Dytiscidae (Coleoptera: Desmopachria chei K. B. Miller y Bidessonotus Régimbart. Además, es la primera ocasión en que se mencionan para la provincia de Jujuy 24 especies y 11 géneros de Coleoptera (Leuronectes Sharp, Bidessonotus, Hypodessus Guignot, Desmopachria Babington, Celina Aubé, Gyrinus Linné, Haliplus Latreille, Hydrocanthus Say, Suphis Aubé, Anacaena Thomson, Derallus Sharp y 19 especies y 12 géneros de Heteroptera (Mesovelia Mulsant & Rey, Hebrus Curtis, Merragata White, Microvelia Westwood, Rhagovelia Mayr, Platyvelia J. T. Polhemus & D. A. Polhemus, Limnogonus Stål, Curicta Stål, Ranatra Fabricius, Centrocorisa Lundblad, Pelocoris Stål, Neoplea Esaki & China.A list of the species of aquatic Coleoptera and Heteroptera occurring in Calilegua National Park is presented. Fifty-one species of Coleoptera included in 28 genera and five families, and 37 species of Heteroptera belonging to 22 genera and 10 families were recorded. Only four species of Coleoptera and five of Heteroptera had been previously cited from the park. Two species of Veliidae (Heteroptera (Rhagovelia trista Gould, Platyvelia brachialis (Stål, along with one species and one genus of Dytiscidae (Coleoptera (Desmopachria chei K. B. Miller, Bidessonotus Régimbart are new records for Argentina. Twenty-four species and 11 genera of Coleoptera (Leuronectes Sharp, Bidessonotus, Hypodessus Guignot, Desmopachria

  6. Coleoptera: Bruchidae

    African Journals Online (AJOL)

    ACSS

    Effect of grain splitting on biology and development of. Callosobruchus ... The developing larvae then bore into the seed prior to harvest. The larvae are the destructive stages inside the grain, causing reduction in quantity and quality of the infested seed. Then after pupation ... cowpea enhanced its market value and shelf life ...

  7. Coleoptera: Curculionidae

    Indian Academy of Sciences (India)

    is no information about its population genetic structure and gene flow patterns. .... and the Fundamental Research Funds for the Central Universities. (78220028). ... A Windows program for the analysis of allozyme and molecular population ...

  8. Coleoptera: Bostrichidae

    African Journals Online (AJOL)

    DR ASSUMING-BREMPONG

    castaneum is worldwide stored product pest ... oil, roots, etc. ... these with similar properties are the Ocimum gratissum (basil), and Ocimum sanctum (holy basil). It is used as an insect repellent during storage. It also works as a mosquito ...

  9. Coleópteros de Colombia: 50 Especies llamativas (Insecta: Coleoptera Un Manual Educativo. Pág. 205-208

    Directory of Open Access Journals (Sweden)

    Maritza Yurieth García Montes

    2013-03-01

    Full Text Available Coleópteros de Colombia- 50 especies llamativas (Insecta: Coleoptera un manual educativo, se presenta como requisito parcial para optar al título de Licenciada en Biología de la UPN, y surge de la necesidad de iniciar programas de sensibilización y movilización frente a la Biota Colombiana, debido a la alta biodiversidad de Colombia, alto grado de amenaza y bajo conocimiento de las especies (Humboldt, 1995. Como docentes, sabemos que la conservación de la biodiversidad es un desafío para la comunidad educativa, y que se requieren más trabajos, que promuevan conocimientos y valores apropiados frente a los recursos naturales. De allí, que el manual educativo, busque acercar a estudiantes, profesores de secundaria y público en general, al conocimiento, importancia y utilidad de los Coleópteros (Insecta: Coleoptera. Estableciéndose como una opción, en el proceso de enseñanza- aprendizaje de la biología, al combinar la investigación biológica y pedagógica alrededor de la escuela.

  10. Investigations on the aquatic Coleoptera (Classis: Insecta fauna of some mountain lakes in the eastern Black Sea range (Turkey.

    Directory of Open Access Journals (Sweden)

    Esat Tarık Topkara

    2015-12-01

    Full Text Available Doğu Karadeniz dağ silsilesindeki bazı dağ göllerinin sucul Coleoptera (Classis: Insecta faunası üzerine araştırmalar. Doğu Karadeniz (Türkiye dağ silsilesindeki yüksek dağ göllerinin sucul Coleoptera faunasını belirlemek amacıyla yapılan bu araştırma, 2005-2007 yıllarında Temmuz ve Ağustos aylarında 22 gölde gerçekleştirilmiştir. Örneklemeler göllerin littoral bölgesinde ve el kepçeleri kullanılarak yapılmıştır. Toplanan örneklerin taksonomik açıdan incelenmesiyle, 4 familyaya ait 21 takson (Dytiscidae 11, Helophoridae 7, Hydraenidae 2, Elmidae 1 tespit edilmiştir

  11. Pretreatment of vinasse from the sugar refinery industry under non-sterile conditions by Trametes versicolor in a fluidized bed bioreactor and its effect when coupled to an UASB reactor.

    Science.gov (United States)

    España-Gamboa, Elda; Vicent, Teresa; Font, Xavier; Dominguez-Maldonado, Jorge; Canto-Canché, Blondy; Alzate-Gaviria, Liliana

    2017-01-01

    During hydrous ethanol production from the sugar refinery industry in Mexico, vinasse is generated. Phenolic compounds and melanoidins contribute to its color and make degradation of the vinasse a difficult task. Although anaerobic digestion (AD) is feasible for vinasse treatment, the presence of recalcitrant compounds can be toxic or inhibitory for anaerobic microorganism. Therefore, this study presents new data on the coupled of the FBR (Fluidized Bed Bioreactor) to the UASB (Upflow Anaerobic Sludge Blanket) reactor under non-sterile conditions by T. versicolor . Nevertheless, for an industrial application, it is necessary to evaluate the performance in this kind of proposal system. Therefore, this study used a FBR for the removal of phenolic compounds (67%) and COD (38%) at non-sterile conditions. Continuous operation of the FBR was successfully for 26 days according to the literature. When the FBR was coupled to the UASB reactor, we obtained a better quality of effluent, furthermore methane content and yield were 74% and 0.18 m 3 CH 4 / kg COD removal respectively. This study demonstrated the possibility of using for an industrial application the coupled of the FBR to the UASB reactor under non-sterile conditions. Continuous operation of the FBR was carried out successfully for 26 days, which is the highest value found in the literature.

  12. Riqueza del complejo chisa (Coleoptera: Melolonthidae en cuatro agroecosistemas del Cauca, Colombia

    Directory of Open Access Journals (Sweden)

    Morón R. Miguel A.

    2005-12-01

    Full Text Available La composición y variación de la riqueza del complejo de larvas Melolonthidae se examinó en cuatro circunstancias agroecológicas en los municipios de Caldono y Buenos Aires, Cauca (1400 a 1500 m.s.n.m, 21,5ºC. y 2000 mm/año de precipitación promedio. Quincenalmente, durante un año, se visitaron parcelas de media hectárea de yuca, pastizal, cafetal y bosque, en cada parcela y ocasión se realizaron 10 muestreos ( 1m2 por 15 cm de profundidad. Se colectaron 10.261 larvas pertenecientes a 32 especies y 12 géneros de Melolonthidae, así: Phyllophaga, Plectris, Astaena, Macrodactylus, Ceraspis, Barybas, Isonychus, Anomala Callistethus, Strigoderma, Leucothyreus, Cyclocephala. La riqueza de especies presentó variaciones estadísticamente significativas entre localidades (F=87.24 p =2.72-18, muestreos (F=22.29 p =5.18-13, parcelas (F= 23.39 p=1.40-13 así como entre la mayoría de interacciones. Los promedios de riqueza fueron: yuca (4.5 , pastizal (3.6, café (2.1 y bosque (2.2, cada uno con grandes fluctuaciones que hicieron la diferencia. Se discuten las curvas de isodensidades de cada parcela y sus implicaciones en el programa de manejo. Abstract Richness of the white-grub complex (Coleoptera: Melolonthidae in agroecosystems of the Cauca province, Colombia. The composition and variation in the richness of the complex of rhizophagous Melolonthidae larvae was examinated in four agroecological zones in the Municipality of Caldono (1400-1500 m, 21.5ºC, average rainfall 2000 mm/yr with complementary surveys in the neighbouring site of Buenos Aires, Cauca. Half-hectare plots (cassava, pastures, coffee and wooded areas were selected and visited every 15 days (total of 15 visits per plot, and a total of 10 samples were taken (1 m2 by 15 cm deep. In total, 10,261 larvae of 32 species were collected: Phyllophaga, Plectris, Astaena, Macrodactylus, Ceraspis, Barybas, Isonychus, Anomala Callistethus, Strigoderma, Leucothyreus, Cyclocephala

  13. Effect of Larval Density on Food Utilization Efficiency of Tenebrio molitor (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Morales-Ramos, Juan A; Rojas, M Guadalupe

    2015-10-01

    Crowding conditions of larvae may have a significant impact on commercial production efficiency of some insects, such as Tenebrio molitor L. (Coleoptera: Tenebrionidae). Although larval densities are known to affect developmental time and growth in T. molitor, no reports were found on the effects of crowding on food utilization. The effect of larval density on food utilization efficiency of T. molitor larvae was studied by measuring efficiency of ingested food conversion (ECI), efficiency of digested food conversion (EDC), and mg of larval weight gain per gram of food consumed (LWGpFC) at increasing larval densities (12, 24, 36, 48, 50, 62, 74, and 96 larvae per dm(2)) over four consecutive 3-wk periods. Individual larval weight gain and food consumption were negatively impacted by larval density. Similarly, ECI, ECD, and LWGpFC were negatively impacted by larval density. Larval ageing, measured as four consecutive 3-wk periods, significantly and independently impacted ECI, ECD, and LWGpFC in a negative way. General linear model analysis showed that age had a higher impact than density on food utilization parameters of T. molitor larvae. Larval growth was determined to be responsible for the age effects, as measurements of larval mass density (in grams of larvae per dm(2)) had a significant impact on food utilization parameters across ages and density treatments (in number of larvae per dm(2)). The importance of mass versus numbers per unit of area as measurements of larval density and the implications of negative effects of density on food utilization for insect biomass production are discussed. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  14. Geometric analysis of nutrient balancing in the mealworm beetle, Tenebrio molitor L. (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Rho, Myung Suk; Lee, Kwang Pum

    2014-12-01

    Geometric analysis of the nutritional regulatory responses was performed on an omnivorous mealworm beetle, Tenebrio molitor L. (Coleoptera: Tenebrionidae) to test whether this beetle had the capacity to balance the intake of protein and carbohydrate. We also identified the pattern of ingestive trade-off employed when the insect was forced to balance the costs of over- and under-ingesting macronutrients. When allowed to mix their diet from two nutritionally imbalanced but complementary foods (protein-biased food: p35:c7 or p28:c5.6; carbohydrate-biased food: p7:c35 or p5.6:c28), beetles of both sexes actively regulated their intake of protein and carbohydrate to a ratio of 1:1. When confined to one of seven nutritionally imbalanced foods (p0:c42, p7:c35, p14:c28, p21:c21, p28:c14, p35:c7 or p42:c0), beetles over-ingested the excessive nutrient from these foods to such an extent that all the points of protein-carbohydrate intake aligned linearly in the nutrient space, a pattern that is characteristic of generalist feeders and omnivores. Under the restricted feeding conditions, males ate more nutrients but were less efficient at retaining their body lipids than females. Body lipid content was higher on carbohydrate-rich foods and was positively correlated with starvation resistance. Our results are consistent with the prediction based on the nutritional heterogeneity hypothesis, which links the nutritional regulatory responses of insects to their diet breadth and feeding ecology. Copyright © 2014. Published by Elsevier Ltd.

  15. Reproductive Plasticity of an Invasive Insect Pest, Rice Water Weevil (Coleoptera: Curculionidae).

    Science.gov (United States)

    Huang, Yunshang; Ao, Yan; Jiang, Mingxing

    2017-12-05

    Reproductive plasticity is a key determinant of species invasiveness. However, there are a limited number of studies addressing this issue in exotic insects. The rice water weevil Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), which is native to North America, is one of the most invasive insect pests in east Asia. In this study, we investigated the reproductive status of first-generation females (progeny of overwintered weevils) from five geographic regions in southern and northern China in the field, and reproductive status and ovipositional features of females provided with suitable host plants in the laboratory after collection. Under field conditions, a proportion of females oviposited, while the rest remained in diapause from all three southern regions investigated, but reproductive development did not take place in females from the two northern regions, where the weevil produces only one generation per year. However, when fed host plants in the laboratory, females from all regions laid eggs. They typically had a very short ovipositional period (3-6 d on average on rice at a temperature of 27°C), laid a low number of eggs, and did not die soon after oviposition; this was different from common reproductive females. We concluded that first-generation L. oryzophilus females, which largely enter diapause after emergence, are highly plastic in their reproductive performance and are ready to reproduce under favorable conditions. Our results indicate the significance of their reproductive plasticity for geographic spread and population development. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  16. Origin of Pest Lineages of the Colorado Potato Beetle (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Izzo, Victor M; Chen, Yolanda H; Schoville, Sean D; Wang, Cong; Hawthorne, David J

    2018-04-02

    Colorado potato beetle (Leptinotarsa decemlineata Say [Coleoptera: Chrysomelidae]) is a pest of potato throughout the Northern Hemisphere, but little is known about the beetle's origins as a pest. We sampled the beetle from uncultivated Solanum host plants in Mexico, and from pest and non-pest populations in the United States and used mitochondrial DNA and nuclear loci to examine three hypotheses on the origin of the pest lineages: 1) the pest beetles originated from Mexican populations, 2) they descended from hybridization between previously divergent populations, or 3) they descended from populations that are native to the Plains states in the United States. Mitochondrial haplotypes of non-pest populations from Mexico and Arizona differed substantially from beetles collected from the southern plains and potato fields in the United States, indicating that beetles from Mexico and Arizona did not contribute to founding the pest lineages. Similar results were observed for AFLP and microsatellite data . In contrast, non-pest populations from the states of Colorado, Kansas, Nebraska, New Mexico, and Texas were genetically similar to U.S. pest populations, indicating that they contributed to the founding of the pest lineages. Most of the pest populations do not show a significant reduction in genetic diversity compared to the plains populations in the United States. We conclude that genetically heterogeneous beetle populations expanded onto potato from native Solanum hosts. This mode of host range expansion may have contributed to the abundant genetic diversity of contemporary populations, perhaps contributing to the rapid evolution of climate tolerance, host range, and insecticide resistance.

  17. Lufenuron impact upon Anthonomus grandis Boheman (Coleoptera: Curculionidae) midgut and its reflection in gametogenesis.

    Science.gov (United States)

    Costa, Hilton Nobre; da Cunha, Franklin Magliano; Cruz, Glaucilane Santos; D'assunção, Carolline Guimarães; Rolim, Guilherme Gomes; Barros, Maria Edna Gomes; Breda, Mariana Oliveira; Teixeira, Alvaro Aguiar Coelho; Teixeira, Valéria Wanderley

    2017-04-01

    The insecticide Match® (lufenuron), one of the main insect growth regulators used in pest control, has been presented as a viable alternative against the boll weevil, Anthonomus grandis (Coleoptera: Curculionidae), by inhibiting chitin synthesis. Thus, this study aimed to examine whether Match® interferes in the synthesis of the peritrophic matrix, leading to changes in the midgut epithelium, resulting in nutritional deficiency and reflecting, thereby, in the gametogenesis process of A. grandis. Floral cotton buds were immersed in the insecticide solution (800μL of Match®+200mL of distilled water) and offered to the adult insects. The midguts of the insects were evaluated after 24 and 120h after feeding. The gonads were evaluated after 120h. The results showed that Match®, in both evaluation periods, induced histopathological alterations such as disorganization, vacuolization and desquamation of the midgut epithelium; histochemical modifications in the distribution patterns of carbohydrates, although without quantitative changes; and a strong decrease in protein levels. No apoptosis were observed, however, there was an increase in the number of regenerative cell nests. In the testicles, a reduction in the amount of spermatozoids and reduced carbohydrate levels were observed, but no difference in protein levels. The ovarioles presented structural disorganization of follicular cells, yolk reduction and decrease in protein levels, however, no change in carbohydrates levels was noted. Therefore, it is concluded that Match® performs histopathologic and histochemical alterations in the midgut epithelium and the gonads of A. grandis adults, reflecting in the gametogenesis process, presenting itself as a promising tool in the management of this pest on cotton crops. Copyright © 2016 Elsevier Inc. All rights reserved.

  18. Relationships of abscised cotton fruit to boll weevil (Coleoptera: Curculionidae) feeding, oviposition, and development.

    Science.gov (United States)

    Showler, Allan T

    2008-02-01

    Abscised cotton, Gossypium hirsutum L., fruit in field plots planted at different times were examined to assess adult boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), use of squares and bolls during 2002 and 2003 in the Lower Rio Grande Valley of Texas. Although boll abscission is not necessarily related to infestation, generally more bolls abscised than squares and abundances of fallen bolls were not related to the planting date treatments. During 2003, fallen squares were most abundant in the late-planted treatment. Although large squares (5.5-8-mm-diameter) on the plant are preferred for boll weevil oviposition, diameter of abscised squares is not a reliable measurement because of shrinkage resulting from desiccation and larval feeding. Fallen feeding-punctured squares and bolls were most abundant in late plantings but differences between fallen feeding-punctured squares versus fallen feeding-punctured bolls were found in only one treatment in 2003. During the same year, fallen oviposition-punctured squares were more numerous in the late-planted treatment than in the earlier treatments. Treatment effects were not found on numbers of oviposition-punctured bolls, but fallen oviposition-punctured squares were more common than bolls in the late-planted treatment compared with earlier treatments each year. Dead weevil eggs, larvae, and pupae inside fallen fruit were few and planting date treatment effects were not detected. Living third instars and pupae were more abundant in fallen squares of the late-planted treatment than in the earlier treatments and bolls of all three treatments. This study shows that fallen squares in late-planted cotton contribute more to adult boll weevil populations than bolls, or squares of earlier plantings.

  19. Comparisons of boll weevil (Coleoptera: Curculionidae) pheromone traps with and without kill strips.

    Science.gov (United States)

    Suh, C P C; Armstrong, J S; Spurgeon, D W; Duke, S

    2009-02-01

    Boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), eradication programs typically equip pheromone traps with an insecticide-impregnated kill strip. These strips are intended to kill captured insects, thereby simplifying trap servicing and reducing the loss of weevils from predation and escape. However, the effectiveness of kill strips has not been extensively evaluated. We examined the influences of kill strips on weevil captures, trap servicing, and the incidences of weevil predation and trap obstruction (e.g., by spider webs). Evaluations were conducted weekly during three different production periods (pre- to early-, late-, and postseason) of cotton, Gossypium hirsutum L., to represent different environmental conditions and weevil population levels. Within each period, mean weekly captures of weevils in traps with and without kill strips were statistically similar. On average, traps with kill strips took 9 s longer to service than traps without kill strips, but statistical differences were only detected during the late-season period. Overall, the mean weekly proportion of traps with evidence of weevil predation or trap obstruction was significantly lower for traps with kill strips (0.25) than for traps without kill strips (0.37). However, this reduction in the frequency of weevil predation or trap obstruction was too small to produce a corresponding increase in the numbers of weevils captured. In light of these findings, the use of kill strips is likely unnecessary in eradication programs, but may be a consideration in situations when the numbers of deployed traps are reduced and chronic problems with weevil predation or trap obstruction exist.

  20. Molecular diagnostic for boll weevil (Coleoptera: Curculionidae) based on amplification of three species-specific microsatellites.

    Science.gov (United States)

    Kim, Kyung Seok; Szendrei, Zsofia; Rodriguez-Saona, Cesar; Mulder, Phillip G; Sappington, Thomas W

    2009-04-01

    The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is a serious pest of cultivated cotton, Gossypium hirsutum L., in the Americas, and reinfestation of zones from which they have been eradicated is of perpetual concern. Extensive arrays of pheromone traps monitor for reintroductions, but occasionally the traps collect nontarget weevils that can be misidentified by scouts. For example, the congeneric pepper weevil, Anthonomus eugenii Cano, and other superficially similar weevils are attracted to components of the boll weevil lure or trap color. Although morphologically distinguishable by trained personnel, the potential for misidentification is compounded when captured weevils are dismembered or partially consumed by ants or ground beetles that sometimes feed on them in the traps. Because misidentification can have expensive consequences, a molecular diagnostic tool would be of great value to eradication managers. We demonstrate that a cocktail of three primer pairs in a single polymerase chain reaction (PCR) amplify species-specific microsatellites that unambiguously distinguish the boll weevil from three other weevil species tested, including pepper weevil; cranberry weevil, Anthonomus eugenii musculus Say; and pecan weevil, Curculio caryae Horn. However, it does not distinguish the boll weevil from the subspecific "thurberia" weevil. A universal internal transcribed spacer primer pair included in the cocktail cross-amplifies DNA from all species, serving as a positive control. Furthermore, the diagnostic primers amplified the target microsatellites from various boll weevil adult body parts, indicating that the PCR technology using the primer cocktail is sensitive enough to positively identify a boll weevil even when the body is partly degraded.

  1. Effectiveness of corn rootworm (Coleoptera: Chrysomelidae) areawide pest management in South Dakota.

    Science.gov (United States)

    French, B Wade; Chandler, Laurence D; Riedell, Walter E

    2007-10-01

    Diabrotica barberi Smith & Lawrence and Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) are serious pests of maize, Zea mays L. To reduce the amount of toxicants released into the environment, the Agricultural Research Service implemented a 5-yr (1997-2001) areawide pest management program in five geographic locations, including one in South Dakota. The objective was to use integrated pest management tactics to suppress adult Diabrotica populations over a broad geographic area by using aerially applied semiochemical-baited insecticides. Suppressed populations theoretically should reduce oviposition, limit larval feeding damage to maize roots, and result in fewer beetles emerging in subsequent years. We used emergence cages, sticky traps, and CRW lure traps to monitor adult D. barberi and D. v. virgifera populations. We sampled for Diabrotica eggs, and we determined damage to maize roots. We sampled in several maize fields (control) located near the areawide site. The baited insecticides were effective in reducing adult populations 1 and 2 wk after application, and most remained low for the duration of the maize growing season. Fewer beetles were captured in both sticky and lure traps in the areawide site than in the control site. With a few exceptions, egg counts, adult emergence, and maize root damage were similar between the areawide and control sites; however, maize roots had greater fresh weight in the control site. Although not all goals were accomplished, when considering the amount of toxicant released into the environment, using semiochemical-baited insecticides to suppress adult pest Diabrotica populations seems to be an effective areawide management tool.

  2. Climatic, Edaphic Factors and Cropping History Help Predict Click Beetle (Coleoptera: Elateridae) (Agriotes spp.) Abundance.

    Science.gov (United States)

    Kozina, A; Lemic, D; Bazok, R; Mikac, K M; Mclean, C M; Ivezić, M; Igrc Barčić, J

    2015-01-01

    It is assumed that the abundance of Agriotes wireworms (Coleoptera: Elateridae) is affected by agro-ecological factors such as climatic and edaphic factors and the crop/previous crop grown at the sites investigated. The aim of this study, conducted in three different geographic counties in Croatia from 2007 to 2009, was to determine the factors that influence the abundance of adult click beetle of the species Agriotes brevis Cand., Agriotes lineatus (L.), Agriotes obscurus (L.), Agriotes sputator (L.), and Agriotes ustulatus Schall. The mean annual air temperature, total rainfall, percentage of coarse and fine sand, coarse and fine silt and clay, the soil pH, and humus were investigated as potential factors that may influence abundance. Adult click beetle emergence was monitored using sex pheromone traps (YATLORf and VARb3). Exploratory data analysis was preformed via regression tree models and regional differences in Agriotes species' abundance were predicted based on the agro-ecological factors measured. It was found that the best overall predictor of A. brevis abundance was the previous crop grown. Conversely, the best predictor of A. lineatus abundance was the current crop being grown and the percentage of humus. The best predictor of A. obscurus abundance was soil pH in KCl. The best predictor of A. sputator abundance was rainfall. Finally, the best predictors of A. ustulatus abundance were soil pH in KCl and humus. These results may be useful in regional pest control programs or for predicting future outbreaks of these species. © The Author 2015. Published by Oxford University Press on behalf of the Entomological Society of America.

  3. Exploring the Leaf Beetle Fauna (Coleoptera: Chrysomelidae of an Ecuadorian Mountain Forest Using DNA Barcoding.

    Directory of Open Access Journals (Sweden)

    Birthe Thormann

    Full Text Available Tropical mountain forests are hotspots of biodiversity hosting a huge but little known diversity of insects that is endangered by habitat destruction and climate change. Therefore, rapid assessment approaches of insect diversity are urgently needed to complement slower traditional taxonomic approaches. We empirically compare different DNA-based species delimitation approaches for a rapid biodiversity assessment of hyperdiverse leaf beetle assemblages along an elevational gradient in southern Ecuador and explore their effect on species richness estimates.Based on a COI barcode data set of 674 leaf beetle specimens (Coleoptera: Chrysomelidae of 266 morphospecies from three sample sites in the Podocarpus National Park, we employed statistical parsimony analysis, distance-based clustering, GMYC- and PTP-modelling to delimit species-like units and compared them to morphology-based (parataxonomic species identifications. The four different approaches for DNA-based species delimitation revealed highly similar numbers of molecular operational taxonomic units (MOTUs (n = 284-289. Estimated total species richness was considerably higher than the sampled amount, 414 for morphospecies (Chao2 and 469-481 for the different MOTU types. Assemblages at different elevational levels (1000 vs. 2000 m had similar species numbers but a very distinct species composition for all delimitation methods. Most species were found only at one elevation while this turnover pattern was even more pronounced for DNA-based delimitation.Given the high congruence of DNA-based delimitation results, probably due to the sampling structure, our study suggests that when applied to species communities on a regionally limited level with high amount of rare species (i.e. ~50% singletons, the choice of species delimitation method can be of minor relevance for assessing species numbers and turnover in tropical insect communities. Therefore, DNA-based species delimitation is confirmed as a

  4. Lectotype designations and nomenclatural changes in Xylographus Mellié (Coleoptera, Ciidae

    Directory of Open Access Journals (Sweden)

    Vivian E. Sandoval-Gómez

    2014-01-01

    Full Text Available We designate lectotypes and propose nomenclatural changes in Xylographus Mellié (Coleoptera, Ciidae based on type specimens deposited in the Museum of Comparative Zoology (USA, Museum für Naturkunde Berlin (Germany, the Natural History Museum (UK, Muséum d’Histoire Naturelle de la Ville de Genève (Switzerland, Muséum National d’Histoire Naturelle (France, Naturhistoriska Riksmuseet (Sweden and Naturhistorisches Museum Wien (Austria. We designate lectotypes for the following species: Cis fultoni Broun, 1886, Xylographus anthracinus Mellié, 1849, X. bicolor Pic, 1916, X. brasiliensis Pic, 1916, X. ceylonicus Ancey, 1876, X. contractus Mellié, 1849, X. corpulentus Mellié, 1849, X. dentatus Pic, 1922, X. gibbus Mellié, 1849, X. hypocritus Mellié, 1849, X. javanus Pic, 1937, X. lemoulti Pic, 1916, X. longicollis Pic, 1922, X. madagascariensis Mellié, 1849, X. nitidissimus Pic, 1916, X. perforatus Gerstaecker, 1871, X. porcus Gorham, 1886, X. punctatus Mellié, 1849, X. ritsemai Pic, 1921, X. rufescens Pic, 1921, X. rufipennis Pic, 1934, X. rufipes Pic, 1930, X. seychellensis Scott, 1926, X. subopacus Pic, 1929, X. subsinuatus Pic, 1916, X. suillus Gorham, 1886, X. testaceitarsis Pic, 1916 and X. tomicoides Reitter, 1902. We propose the following syn. n. (senior synonym listed first: X. anthracinus = X. testaceitarsis, X. brasiliensis = X. lucasi Lopes-Andrade & Zacaro, X. corpulentus = X. lemoulti and X. richardi Mellié, X. madagascariensis = X. eichelbaumi Reitter, X. rufipennis, X. seychellensis Scott and X. tarsalis Fåhraeus, X. nitidissimus = X. longicollis, X. subsinuatus = X. rufescens. We exclude three species from Xylographus: Cis renominatus, nom.n. (for X. dentatus Pic, 1922, not C. dentatus Mellié, 1849, Paratrichapus fultoni (Broun, 1886, comb. n. and P. javanus (Pic, 1937, comb. n.

  5. Efficacy of Systemic Insecticides for Control of the Invasive Goldspotted Oak Borer (Coleoptera: Buprestidae) in California.

    Science.gov (United States)

    Coleman, Tom W; Smith, Sheri L; Jones, Michael I; Graves, Andrew D; Strom, Brian L

    2017-10-01

    From 2009 to 2013, we tested four systemic insecticide formulations and five application methods against the invasive goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), in California. The insecticides were evaluated in three experiments: 1) 2009 remedial applications of emamectin benzoate (stem-injection) and imidacloprid (stem-injection and soil-injection); 2) 2009-2012 emamectin benzoate and imidacloprid initially applied at different times during the dormant season with varying injection technologies; and 3) 2013 dinotefuran applied to several tree diameter size classes. Adult leaf-feeding bioassays were used to assess the impact of systemic treatments against A. auroguttatus, whereas enzyme-linked immunosorbent assays determined the quantity of the active ingredient of insecticide residues in foliage. Imidacloprid (experiment 1) persisted at elevated levels in foliage of coast live oak, Quercus agrifolia Née, for 1.5 yr following stem injections. Stem injections of emamectin benzoate (experiment 2) sometimes significantly decreased survival in adults fed foliage from treated Q. agrifolia, and both the emamectin benzoate and imidacloprid treatments reduced adult feeding in some trials. Imidacloprid residues in Q. agrifolia and California black oak, Quercus kelloggii Newb., foliage remained at elevated levels (>10 µg/g) ∼2 yr postapplication. In 2013 (experiment 3), dinotefuran residues were highest in foliage collections 2 wk postapplication and greatest in smaller diameter oaks, but insecticide treatment had no effect on survival or frass production by adults fed foliage from treated trees. Systemic injections of emamectin benzoate and imidacloprid applied during the dormant season to uninfested or lightly infested oaks can reduce adult A. auroguttatus survival and maturation feeding. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by (a) US Government employee

  6. A importância forense de Oxelytrum discicolle (Brullé, 1840 (Coleoptera, Silphidae

    Directory of Open Access Journals (Sweden)

    Camila Kraiczyi Kotzko

    2015-08-01

    Full Text Available Oxelytrum discicolle Brullé, 1840 é a espécie mais coletada de Silphidae (Coleoptera na América Latina, constituindo um indicador forense na região Neotropical. O objetivo do trabalho é reunir informações sobre biologia e distribuição de O. discicolle, além de ferramentas para utilização desse besouro como indicador forense em casos de morte de humanos. O. discicolle é um besouro de grande porte, de cor preta com um colar vermelho. Larvas da espécie são necrófagas, já os adultos são onívoros e se alimentam tanto de larvas de dípteros quanto da própria carcaça. Nossa revisão aponta que a espécie reduz seu tempo de desenvolvimento conforme a temperatura aumenta, sugerindo que a taxa de desenvolvimento é diferente entre as temperaturas. Estudos mostram que a espécie O. discicolle é um forte indicador forense, porém para que ocorra o avanço das pesquisas de Entomologia Forense em humanos no Brasil é necessário que os Institutos de Criminalística publiquem mais dados sobre insetos que os colonizam. Além disso, são necessários mais estudos em carcaças de animais em diversas regiões e biomas diferentes, desta forma pode ser montado um mapa de distribuição da espécie em nível nacional.

  7. Azadirachtin: an effective systemic insecticide for control of Agrilus planipennis (Coleoptera: Buprestidae).

    Science.gov (United States)

    McKenzie, Nicole; Helson, Blair; Thompson, Dean; Otis, Gard; McFarlane, John; Buscarini, Teresa; Meating, Joe

    2010-06-01

    The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive pest discovered in North America in 2002, is now well established and threatens ash (Fraxinus spp.) trees throughout the continent. Experiments were conducted to 1) examine the efficacy of an alternative natural pesticide, azadirachtin, to control emerald ash borer, and 2) determine foliar uptake and dissipation patterns after systemic injections of azadirachtin into trunks of small (2.2 cm diameter at breast height [dbh]), uninfested green ash trees. We found no evidence of mortality of adult beetles. In contrast, fewer larvae completed their development at dose levels > or = 1.7 mg (AI)/cm dbh and development ceased beyond the second instar at dose levels > or = 13.6 mg (AI)/cm dbh. Substantial concentrations (11.2 microg/g dry mass [SD = 7.55]) of azadirachtin were present in leaves within 7 d of treatment. After rapid initial uptake, concentrations in leaves declined logarithmically during the 55 d after injection. A similar pattern was observed in a separate experiment that examined the uptake and translocation of azadirachtin in larger green ash trees (22 cm dbh) treated with 250 mg (AI) /cm dbh with the EcoJect injection system. In another experiment, recently infested plantation green ash trees treated with doses > or = 40 mg (AI)/cm dbh had significant reductions in adult emergence approximately 1 yr postinjection. Given the inhibition of larval development, reduction of adult emergence, and the occurrence of foliar residues at biologically active concentrations, we conclude that azadirachtin is effective in protecting ash trees from emerald ash borer.

  8. Chemosensory gene families in adult antennae of Anomala corpulenta Motschulsky (Coleoptera: Scarabaeidae: Rutelinae.

    Directory of Open Access Journals (Sweden)

    Xiao Li

    Full Text Available The metallic green beetle, Anomala corpulenta (Coleoptera: Scarabaeidae: Rutelinae, is a destructive pest in agriculture and horticulture throughout Asia, including China. Olfaction plays a crucial role in the survival and reproduction of A. corpulenta. As a non-model species, A. corpulenta is poorly understood, and information regarding the molecular mechanisms underlying olfaction in A. corpulenta and other scarab species is scant.We assembled separate antennal transcriptome for male and female A. corpulenta using Illumina sequencing technology. The relative abundance of transcripts with gene ontology annotations, including those related to olfaction in males and females was highly similar. Transcripts encoding 15 putative odorant binding proteins, five chemosensory proteins, one sensory neuron membrane protein, 43 odorant receptors, eight gustatory receptors, and five ionotropic receptors were identified. The sequences of all of these chemosensory-related transcripts were confirmed using reverse transcription polymerase chain reaction (RT-PCR, and direct DNA sequencing. The expression patterns of 54 putative chemosensory genes were analyzed using quantitative real time RT-PCR (qRT-PCR. Antenna-specific expression was detected for many of these genes, suggesting that they may have important functions in semiochemical detection.The identification of a large number of chemosensory proteins provides a major resource for the study of the molecular mechanism of odorant detection in A. corpulenta and its chemical ecology. The genes identified, especially those that were expressed at high levels in the antennae may represent novel molecular targets for the development of population control strategies based on the manipulation of chemoreception-driven behaviors.

  9. Variability in Small Hive Beetle (Coleoptera: Nitidulidae) Reproduction in Laboratory and Field Experiments.

    Science.gov (United States)

    Meikle, William G; Holst, Niels; Cook, Steven C; Patt, Joseph M

    2015-06-01

    Experiments were conducted to examine how several key factors affect population growth of the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae). Laboratory experiments were conducted to examine effects of food quantity and temperature on reproduction of cohorts of young A. tumida adults (1:1 sex ratio) housed in experimental arenas. Daily numbers and total mass of larvae exiting arenas were highly variable within treatment. Either one or two cohorts of larvae were observed exiting the arenas. Food quantity, either 10 g or 20 g, did not significantly affect the number of larvae exiting arenas at 32°C, but did at 28°C; arenas provided 20 g food produced significantly more larvae than arenas provided 10 g. Temperature did not affect the total mass of larvae provided 10 g food, but did affect larval mass provided 20 g; beetles kept at 28°C produced more larval mass than at 32°C. Field experiments were conducted to examine A. tumida reproductive success in full strength bee colonies. Beetles were introduced into hives as egg-infested frames and as adults, and some bee colonies were artificially weakened through removal of sealed brood. Efforts were unsuccessful; no larvae were observed exiting from, or during the inspection of, any hives. Possible reasons for these results are discussed. The variability observed in A. tumida reproduction even in controlled laboratory conditions and the difficulty in causing beetle infestations in field experiments involving full colonies suggest that accurately forecasting the A. tumida severity in such colonies will be difficult. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  10. Sulfuryl fluoride as a quarantine treatment for Chlorophorus annularis (Coleoptera: Cerambycidae) in Chinese bamboo poles.

    Science.gov (United States)

    Yu, Daojian; Barak, Alan V; Jiao, Yi; Chen, Zhinan; Zhang, Guiming; Chen, Zhilin; Kang, Lin; Yang, Weidong

    2010-04-01

    Bamboo (genera Bambusa and Phyllstachys) is one of the fastest growing and economically important plants in the world, and it is cultivated widely throughout southern China. China annually exports to the United States significant quantities of bamboo garden stakes (Bambusa spp.). In recent years, Plant Protection and Quarantine officers of the U.S. Department of Agriculture-Animal and Plant Health Inspection Service have made numerous interceptions of the bamboo borer, Chlorophorus annularis Fairmaire (Coleoptera: Cerambycidae), in bamboo products from China. This species is considered to have high pest risk potential in the trade of bamboo products. As a fumigant, sulfuryl fluoride (SF) would be a practical alternative to methyl bromide (MeBr) fumigation. Here, we report the results of SF fumigation tests for C. annularis in bamboo poles at three doses--96 g/m3 at 15.9 degrees C, 80 g/m3 at 21.5 degrees C, and 64 g/m3 at 26.0 degrees C--in glass test chambers. Commercial standard fumigations were also conducted in a standard 6.1-m-long, 33.2-m3 (standard height, 20-feet) marine general cargo container loaded to 80% (vol:vol) with similar bamboo poles, and sufficient levels of SF were obtained during the 24-h fumigations. During the course of these tests, 2424 larvae, 90 pupae, and 23 adults in total were killed, with no survivors. A treatment schedule using SF is proposed for bamboo as an alternative to MeBr at several temperatures tested.

  11. Influence of trap color and host volatiles on capture of the emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Crook, Damon J; Khrimian, Ashot; Cossé, Allard; Fraser, Ivich; Mastro, Victor C

    2012-04-01

    Field trapping assays were conducted in 2009 and 2010 throughout western Michigan, to evaluate lures for adult emerald ash borer, A. planipennis Fairmaire (Coleoptera: Buprestidae). Several ash tree volatiles were tested on purple prism traps in 2009, and a dark green prism trap in 2010. In 2009, six bark oil distillate lure treatments were tested against manuka oil lures (used in 2008 by USDA APHIS PPQ emerald ash borer cooperative program). Purple traps baited with 80/20 (manuka/phoebe oil) significantly increased beetle catch compared with traps baited with manuka oil alone. In 2010 we monitored emerald ash borer attraction to dark green traps baited with six lure combinations of 80/20 (manuka/phoebe), manuka oil, and (3Z)-hexenol. Traps baited with manuka oil and (3Z)-hexenol caught significantly more male and total count insects than traps baited with manuka oil alone. Traps baited with manuka oil and (3Z)-hexenol did not catch more beetles when compared with traps baited with (3Z)-hexenol alone. When compared with unbaited green traps our results show that (3Z)-hexenol improved male catch significantly in only one of three field experiments using dark green traps. Dark green traps caught a high number of A. planipennis when unbaited while (3Z)-hexenol was seen to have a minimal (nonsignificant) trap catch effect at several different release rates. We hypothesize that the previously reported kairomonal attractancy of (3Z)-hexenol (for males) on light green traps is not as obvious here because of improved male attractancy to the darker green trap.

  12. Evaluation of heat treatment schedules for emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Myers, Scott W; Fraser, Ivich; Mastro, Victor C

    2009-12-01

    The thermotolerance of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), was evaluated by subjecting larvae and prepupae to a number of time-temperature regimes. Three independent experiments were conducted during 2006 and 2007 by heating emerald ash borer infested firewood in laboratory ovens. Heat treatments were established based on the internal wood temperature. Treatments ranged from 45 to 65 degrees C for 30 and 60 min, and the ability of larvae to pupate and emerge as adults was used to evaluate the success of each treatment. A fourth experiment was conducted to examine heat treatments on exposed prepupae removed from logs and subjected to ambient temperatures of 50, 55, and 60 degrees C for 15, 30, 45, and 60 min. Results from the firewood experiments were consistent in the first experiment. Emergence data showed emerald ash borer larvae were capable of surviving a temperatures-time combination up to 60 degrees C for 30 min in wood. The 65 degrees C for 30 min treatment was, however, effective in preventing emerald ash borer emergence on both dates. Conversely, in the second experiment using saturated steam heat, complete mortality was achieved at 50 and 55 degrees C for both 30 and 60 min. Results from the prepupae experiment showed emerald ash borer survivorship in temperature-time combinations up to 55 degrees C for 30 min, and at 50 degrees C for 60 min; 60 degrees C for 15 min and longer was effective in preventing pupation in exposed prepupae. Overall results suggest that emerald ash borer survival is variable depending on heating conditions, and an internal wood temperature of 60 degrees C for 60 min should be considered the minimum for safe treatment for firewood.

  13. Sequential Sampling Plan of Anthonomus grandis (Coleoptera: Curculionidae) in Cotton Plants.

    Science.gov (United States)

    Grigolli, J F J; Souza, L A; Mota, T A; Fernandes, M G; Busoli, A C

    2017-04-01

    The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is one of the most important pests of cotton production worldwide. The objective of this work was to develop a sequential sampling plan for the boll weevil. The studies were conducted in Maracaju, MS, Brazil, in two seasons with cotton cultivar FM 993. A 10,000-m2 area of cotton was subdivided into 100 of 10- by 10-m plots, and five plants per plot were evaluated weekly, recording the number of squares with feeding + oviposition punctures of A. grandis in each plant. A sequential sampling plan by the maximum likelihood ratio test was developed, using a 10% threshold level of squares attacked. A 5% security level was adopted for the elaboration of the sequential sampling plan. The type I and type II error used was 0.05, recommended for studies with insects. The adjustment of the frequency distributions used were divided into two phases, so that the model that best fit to the data was the negative binomial distribution up to 85 DAE (Phase I), and from there the best fit was Poisson distribution (Phase II). The equations that define the decision-making for Phase I are S0 = -5.1743 + 0.5730N and S1 = 5.1743 + 0.5730N, and for the Phase II are S0 = -4.2479 + 0.5771N and S1 = 4.2479 + 0.5771N. The sequential sampling plan developed indicated the maximum number of sample units expected for decision-making is ∼39 and 31 samples for Phases I and II, respectively. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  14. CARABID BEETLES FAUNA (COLEOPTERA, CARABIDAE OF THE TSHETSHEN ISLAND IN THE CASPIAN SEA

    Directory of Open Access Journals (Sweden)

    I. A. Belousov

    2014-01-01

    Full Text Available Aim. Carabid species (Coleoptera, Carabidae are listed for Tshetshen Island in the Caspian Sea, Dagestan. The study is based on examination of 32799 adult carabids belonging to 123 species from 49 genera collected in 2011-1013. One species - Sirdenus grayii (Wollaston, 1862 – is firstly recorded from the territory ofDagestan.Location. Materials for the work served copies for the imago carabids,collected on the Chechen island in 2011-2012 years as staff and the students of ecologo-geografical faculty of Dagistan State University and the Institute for Applied Ecology (Makhachkala Methods. Charges were made with the help of light traps, soil traps, including trap, enhanced light source .Geografpical coordinates of all locations were recorded using GPS- navigator: T1 - 43°57’58” N 47°38’35” E; T2 - 43°58’17” N 47°42’55”; T3 - 43°59’08” N 47°44’39” E; T4 - 43°57’27” N 47°45’05” E; Лагерь - 43°58’11”N47°38’46” E. Results. As a result of investigations indentified the species composition of the carabids of the island Chechen.Main conclusions. Total hectares of the Chechen island collected 32799 copies of the carabids, belonging to 123 species. Sirdenus grayii (Wollaston, 1862 –for the first time actuated in Dagestan.

  15. Scarab Beetle (Coleoptera: Scarabaeidae Fauna in Ardabil Province, North West Iran

    Directory of Open Access Journals (Sweden)

    G Mowlavi

    2008-12-01

    Full Text Available "nBackground: Dung beetles of Coleoptera associated to undisturbed cattle droppings in pastures present great diver¬sity and abundance. Dung beetles also play an important role for transmission of some helminthes to human and cat¬tle. This study was made to survey the biodiversity and abundance of these beetles in Ardebil Province, western Iran."nMethods: According to the field study all beetles attracted to fresh cow dung in five areas of Ardebil Province in¬cluding Namin, Ardabil, Meshkinshahr, Neer and Sarein were collected and identified. They were collected during summer 2007 from June to September, with general peaks appearing to be correlated with temperature mainly at 11 a.m to 15 p.m. The samples were identified using appropriate systematic key "nResults: A total of 231 specimens belonging to 9 beetle genera and at least 15 species were identified as Euoniticel¬lus fulvus, Sisyphus schaffaer, Euonthophagus taurus, Copris lunaris, Chironitis pamphilus, Gymnopleurus coriarus, Euonthophagus amyntas, Caccobius schreberi, Onthophagus speculifer, Onthophagus furcatus, Aphodius, lugens, Apho¬dius fimetarius, A. scrutator, Geotrupes spiniger and G. stercorarius"nThe most abundant and diverse subfamilies were Coprinae, Geotrupinae, and Aphodiinae. "nConclusion: We found 15 species of dung beetles occurred in the region. The prevalence of each species is varied depending on location. Some of them play an important role for helminths transmission of veterinary and public health importance. The finding will provide a clue for pasture management as well as public health monitoring and surveillance of the disease transmitted by dung beetles. 

  16. Dung beetles (Coleoptera: Scarabaeoidea in three landscapes in Mato Grosso do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    MM. Rodrigues

    Full Text Available Dung beetles (Coleoptera: Scarabaeoidea in three landscapes in Mato Grosso do Sul, Brazil. Dung Beetles are important for biological control of intestinal worms and dipterans of economic importance to cattle, because they feed and breed in dung, killing parasites inside it. They are also very useful as bioindicators of species diversity in agricultural or natural environments. The aims of this paper were to study the species richness, and abundance of dung beetles, helping to answer the question: are there differences in the patterns of dung beetle diversity in three environments (pasture, agriculture and forest in the municipality of Dourados, in the state of Mato Grosso do Sul. A total of 105 samplings were carried out weekly, from November 2005 to November 2007, using three pitfall traps in each environment. The traps were baited with fresh bovine dung, and 44,355 adult dung beetles from 54 species were captured: two from Hyborosidae and 52 from Scarabaeidae. Five species were constant, very abundant and dominant on the pasture, two in the agricultural environment, and two in the environment of Semideciduous forest. Most of the species were characterised as accessories, common and not-dominant. The species with higher abundance was Ataenius platensis Blanchard, 1844. The indexes of Shannon-Wiener diversity were: 2.90 in the pasture, 2.84 in the agricultural environment and 2.66 in the area of native forest. The medium positive presence of dung beetles in the traps in each environment were: 36.88, 42.73 and 20.18 individuals per trap, in the pasture, agricultural environment and in the native forest, respectively. The pasture environment presented a higher diversity index. The species diversity of dung beetles was superior where there was higher abundance and regularity of resource (bovine dung.

  17. Seasonal and nocturnal activities of the rhinoceros borer (Coleoptera: Scarabaeidae) in the north Saharan oases ecosystems.

    Science.gov (United States)

    Ehsine, M'hammed; Belkadhi, Mohamed Sadok; Chaieb, Mohammed

    2014-01-01

    The rhinoceros borer Oryctes agamemnon Burmeister (Coleoptera: Scarabaeidae) is a date palm insect pest that causes damage to trunk and roots of palm trees in several countries, including Tunisia, the United Arab Emirates (UAE), Oman, and Saudi Arabia. The aim of this study was to monitor the seasonal and nocturnal activities of this beetle. Experiments were performed on a date palm of Rjim Maatoug during a 6-yr period (2004-2007, 2009-2010). Field survey using light traps shows that O. agamemnon is a univoltine, with a single population peak. Adults appear in the field around late May-early June and the population continued to build until maximum numbers are reached between the end of July and the beginning of August in the same year. No adults were found after first 10 d of November. This peak was characterized by female dominance in number. The monitoring of nocturnal activity showed that it starts its activities roughly 40 min after the sundown and continues until approximately 1 h before sunrise. The highest number of trapped beetles was remarked in the two first hours of flight activity, with a dominance of female in the first hour and a dominance of male in the second hour. We remarked that the sex ratio (female:male) of the cumulated number of trapped adults in the different years and nights of survey was in favor of females. © The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America.

  18. BIOECOLOGIA E MANEJO DA BROCA-DA-ERVA-MATE, Hedypathes betulinus (KLUG, 1825 (COLEOPTERA: CERAMBYCIDAE

    Directory of Open Access Journals (Sweden)

    Jerson V. Carús Guedes

    2006-07-01

    Full Text Available A cultura da erva-mate (Ilex paraguariensis A.St.-Hil. apresenta grande importância social, cultural e econômica no sul do Brasil. Com a formação de povoamentos puros e o aumento da área de plantio, observou-se um aumento populacional de muitas espécies de insetos. Dentre os insetos-praga, a broca-da-erva-mate, Hedypathes betulinus (Klug, 1825 (Coleoptera: Cerambycidae, é a de maior importância econômica, em razão dos danos que causa e seu difícil controle. A fase larval ocorre no interior dos galhos e troncos das plantas, dificultando sua detecção e manejo. O manejo cultural e o mecânico são os mais indicados, como a coleta de adultos, poda e queima de partes atacadas da planta, nutrição equilibrada, densidade adequada de plantas e manutenção de áreas com vegetação nativa ou ainda a introdução do policultivo. Com essas técnicas, espera-se um maior equilibrio do agroecossistema e, com isso, a diminuição dos insetos-praga a um nível aceitável. Portanto, o objetivo deste trabalho é reunir e discutir as informações da bioecologia e manejo da broca-da-erva-mate.

  19. Mass Trapping and Classical Biological Control of Rhynchophorus palmarum L. 1794 (Coleoptera: Curculionidae. A hypothesis based in morphological evidences.

    Directory of Open Access Journals (Sweden)

    Flávio Miguens

    2011-07-01

    Resumo. Coleópteros têm sido descritos como pragas e vetores de nematódeos causadores de Anel Vermelho em diversas palmeiras da família Arecaceae. Rhynchophorus palmarum L (Coleoptera: Curculionidae é uma praga que afeta a cocoicultura e outras palmeiras; e, vetor de Bursaphelencus cocophilus (Cobb Baujard (Nematoda, agente etiológico de Anel Vermelho e de outros nematódeos. Atualmente, recomenda-se o emprego de inimigos naturais e parasitas no manejo integrado de pragas; dentre elas, Rhynchophorinae. Armadilhas de coleta massal são recomendadas no manejo integrado de pragas. Nosso estudo relata, na cocoicultura, a eficiência de armadilhas artesanais de baixo custo e a utilização cariomônios (toletes de cana-de-açúcar e cariômonios mais feromônios (toletes de cana-de-açúcar e machos adultos de R. palmarum como atrativos nas armadilhas. Ácaros ectoparasitas foram identificados nestes coleópteros, por microscopia, que podem ser propostos como parte do manejo integrado desta praga. Armadilhas de coleta massal com cariômonios foram eficientes na captura de R. palmarum e outros Curculionidae. No entanto, armadilhas de coleta massal com cariômonios e feromônios aumentaram a atratividade, em relação às primeiras, para este Coleoptera e Cosmopolites sordidus (Germar (Coleoptera: Curculionidae. Eventualmente, exemplares de R. palmarum apresentavam infecção fúngica. Ácaros ectoparasitas infestavam mais de 50% dos exemplares de R. palmarum. A microanatomia destes ácaros e sua interação com R. palmarum foi preliminarmente descrita. Todos os estágios do ciclo de vida destes ácaros foram identificados no compartimento dos élitros. As evidências morfológicas suportam a hipótese de que estes ácaros podem ser empregados no controle biológico de R. palmarum em um programa de manejo integrado.

  20. A cure for the blues: opsin duplication and subfunctionalization for short-wavelength sensitivity in jewel beetles (Coleoptera: Buprestidae).

    Science.gov (United States)

    Lord, Nathan P; Plimpton, Rebecca L; Sharkey, Camilla R; Suvorov, Anton; Lelito, Jonathan P; Willardson, Barry M; Bybee, Seth M

    2016-05-18

    Arthropods have received much attention as a model for studying opsin evolution in invertebrates. Yet, relatively few studies have investigated the diversity of opsin proteins that underlie spectral sensitivity of the visual pigments within the diverse beetles (Insecta: Coleoptera). Previous work has demonstrated that beetles appear to lack the short-wavelength-sensitive (SWS) opsin class that typically confers sensitivity to the "blue" region of the light spectrum. However, this is contrary to established physiological data in a number of Coleoptera. To explore potential adaptations at the molecular level that may compensate for the loss of the SWS opsin, we carried out an exploration of the opsin proteins within a group of beetles (Buprestidae) where short-wave sensitivity has been demonstrated. RNA-seq data were generated to identify opsin proteins from nine taxa comprising six buprestid species (including three male/female pairs) across four subfamilies. Structural analyses of recovered opsins were conducted and compared to opsin sequences in other insects across the main opsin classes-ultraviolet, short-wavelength, and long-wavelength. All nine buprestids were found to express two opsin copies in each of the ultraviolet and long-wavelength classes, contrary to the single copies recovered in all other molecular studies of adult beetle opsin expression. No SWS opsin class was recovered. Furthermore, the male Agrilus planipennis (emerald ash borer-EAB) expressed a third LWS opsin at low levels that is presumed to be a larval copy. Subsequent homology and structural analyses identified multiple amino acid substitutions in the UVS and LWS copies that could confer short-wavelength sensitivity. This work is the first to compare expressed opsin genes against known electrophysiological data that demonstrate multiple peak sensitivities in Coleoptera. We report the first instance of opsin duplication in adult beetles, which occurs in both the UVS and LWS opsin classes

  1. Distribución y patrones de diversidad de los Afódidos en la Comunidad de Madrid (Coleoptera, Scarabaeoidea, Aphodiidae, Aphodiinae y Psammodiinae

    Directory of Open Access Journals (Sweden)

    Hortal, J.

    2006-05-01

    Full Text Available In this work we present an updated checklist of the Aphodiidae (Coleoptera, Scarabaeoidea from Comunidad de Madrid (Spain. In addition, the observed and potential distributions of the 70 species found in Madrid are mapped. The potential spatial distributions of species richness, rarity and endemism in this region are also mapped. Finally, we discuss briefly the origin of the observed patterns.

    En este trabajo se presenta un inventario actualizado de los Aphodiidae (Coleoptera, Scarabaeoidea de la Comunidad de Madrid. Además, se presentan mapas de la distribución observada y potencial de las 70 especies encontradas en Madrid, así como de la distribución espacial de la riqueza, rareza, y endemicidad potencial en la región. Finalmente, se discute brevemente el origen de los patrones observados.

  2. Karagöl (Yamanlar, İzmir'ün Sucul Coleoptera (Classis: Insecta Faunasına Katkılar.

    Directory of Open Access Journals (Sweden)

    Esat Tarık Topkara

    2015-12-01

    Full Text Available Bu çalışmada, Karagöl (Yamanlar-İzmir’den 18 Mayıs 2006 tarihinde yapılan örnekleme sonucu elde edilen sucul Coleoptera örnekleri değerlendirilmiştir. Sistematik açıdan yapılan değerlendirmede, Adephaga altfamilyasının Gyrinidae ve Dytiscidae familyalarına ait 4 tür, Polyphaga altfamilyasının Helophoridae, Hydrophilidae, Hydraenidae ve Dryopidae familyalarına ait 7 tür tespit edilmiştir. Tespit edilen türler bu gölün sucul Coleoptera faunası için yeni kayıt niteliğindedir

  3. An annotated catalogue of the type material of Elateroidea Leach, 1815 (Coleoptera) deposited in the Coleoptera collection of the Museum of Zoology of the University of São Paulo, Brazil.

    Science.gov (United States)

    Ferreira, Vinicius De Souza

    2015-03-25

    The Museum of Zoology of the University of São Paulo (MZSP) houses one of the most important Coleoptera collections of Brazil and Neotropical Region with nearly 900,000 adult mounted material and about 1,500,000 specimens to be mounted. The superfamily Elateroidea Leach, 1815 (including Cantharoidea) comprises about 24,077 described species in 17 families. The MZSP owns type material of Brachypsectridae LeConte & Horn, 1883, Cantharidae, 1856 (1815), Cerophytidae Latreille, 1834, Elateridae Leach 1815, Eucnemidae Eschscholtz, 1829, Lampyridae Rafinesque, 1815, Lycidae Laporte, 1836, Phengodidae LeConte, 1861 and Rhinorhipidae Lawrence, 1988. This catalogue includes type material of 166 species distributed in 69 genera. Among 1,223 type specimens, are 86 holotypes, 1,133 paratypes, 2 allotypes, 1 lectotype and 1 paralectotype.

  4. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae. A atividade inseticida de Toona sureni (Blume Merr. foi avaliada considerando repelência, mortalidade e a produção de progênie de adultos F1 de Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Extrato seco de sementes

  5. A diversidade inventarial de Coleoptera (Insecta em uma paisagem antropizada do Bioma Araucária The inventory diversity of Coleoptera (Insecta of an anthropized landscape in the Biome Araucaria

    Directory of Open Access Journals (Sweden)

    Norma G. Ganho

    2005-12-01

    Full Text Available Como parte do Projeto PROVIVE, foram analisadas a riqueza de espécies, a composição taxonômica, a proporção de espécies raras e a constância taxonômica ao nível de família relacionada à riqueza de espécies, em comunidades de Coleoptera, em Vila Velha, Ponta Grossa, Paraná. Os dados foram obtidos a partir de coletas através de armadilha malaise no estrato do sub-bosque de cinco áreas com diferentes graus de intervenção antrópica, de setembro de 1999 a agosto de 2000. As 52 semanas de amostragem nas cinco áreas resultaram na coleta de 10.822 indivíduos de 1659 espécies. Todas as áreas apresentaram alta riqueza de espécies e diversidade, como indicado por vários índices. A área em estágio mais avançado de sucessão vegetal foi menos rica do que aquelas em estágio inicial/intermediária. De acordo com diferentes estimadores de riqueza de espécies, o número de espécies coletadas poderia aumentar de 22-123% com o aumento do esforço de coleta. As áreas menos conservadas foram mais ricas em espécies raras ("singletons", "doubletons" e únicas do que as mais conservadas. Nas cinco áreas houve uma constância taxonômica entre as famílias mais ricas (Curculionidae, Chrysomelidae, Cerambycidae, Staphylinidae, Mordelidae, Elateridae, Scarabaeidae, Coccinellidae e Tenebrionidae envolvendo 60% do total de espécies, como observado para a abundância de indivíduos. A existência de um padrão de constância taxonômica de famílias, quando considerados 60% da riqueza de espécies e/ou de abundância de indivíduos por local, poderá tornar mais fácil e rápido o estudo de comunidades de Coleoptera, habilitando a ordem a ser um táxon indicador de condições ambientais de áreas florestadas.The species richness, taxonomic composition, rare species, and taxonomic constancy at family level were studied in communities of Coleoptera in Vila Velha, Ponta Grossa, Paraná, as part of PROVIVE project. The data were gathered

  6. CONTRIBUTION FOR THE DIAGNOSIS AND CONTROL OF THE BARK BEETLES OF GENUS IPS (COLEOPTERA: SCOLYTIDAE) IN THE PINE FOREST OF CUBA

    OpenAIRE

    René Alberto López Castilla; Fidel Góngora Rojas; Celia Guerra Rivero; Enrique de Zayas Izaguirre; Antonio Fernández Vera; Natividad Triguero Isasi

    2009-01-01

    There are four pines species endemic from Cuba with width importance, from the Conservation of the Forest Genetic Resources at regional level to the mitigation of the climatic change. Their economical importance is due to forming pure forest stand of fast growth and of straight trunk. The bark beetle of the genus Ips De Geer (Coleoptera: Scolytidae) and the associate mushrooms from the complex Ophiostomatoid (Ceratocystidaceae: Microascales Phylum Ascomycotina) are those that cause the bigge...

  7. Development and survival of the spruce bark beetle, Ips typographus (Coleoptera: Curculionidae: Scolytinae) at low temperatures in the laboratory and the field

    Czech Academy of Sciences Publication Activity Database

    Štefková, Kristýna; Okrouhlík, Jan; Doležal, Petr

    2017-01-01

    Roč. 114, JAN 11 (2017), s. 1-6 E-ISSN 1802-8829 Grant - others:MSM ČR(CZ) LH12098; Lesy ČR(CZ) 08/2009 Institutional support: RVO:60077344 Keywords : Coleoptera * Curculionidae * Scolytinae Subject RIV: ED - Physiology OBOR OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology Impact factor: 1.167, year: 2016 http://www.eje.cz/pdfs/eje/2017/01/01.pdf

  8. First record of Sinoxylon anale and S. unidentatum in Greece, with an updated account on their global distribution and host plants (Coleoptera: Bostrichidae

    Directory of Open Access Journals (Sweden)

    Charalampos T. Lykidis

    2016-12-01

    Full Text Available Sinoxylon anale Lesne, 1897 and S. unidentatum (Fabricius, 1801 (Coleoptera, Bostrichidae, two almost cosmopolitan species most likely native of the Oriental Region, are recorded for the first time from Greece on the basis of several specimens intercepted in a consignment at the Piraeus harbor (Attica, Athens in wood packaging material originating from China. The establishment of these species in Greece is briefly discussed, moreover, an updated list of their interceptions, countries of establishment and host plants, is provided.

  9. CEPF Western Ghats Special Series : Parasitoids (Hymenoptera of xylophagous beetles (Coleoptera attacking dead wood in southern Western Ghats, Kerala, India, with descriptions of two new species

    Directory of Open Access Journals (Sweden)

    P.M. Sureshan

    2013-05-01

    Full Text Available An account is given of four species of Hymenoptera parasitoids probably of the wood boring beetle Clytocera chinospila Gahan (Coleoptera: Cerambycidae from Chinnar Wildlife Sanctuary, southern Western Ghats, Kerala. Two new hymenopteran species, Eurytoma chinnarensis (Eurytomidae and Foenatopus idukkiensis (Stephanidae are described. Solenura ania Walker (Pteromalidae is reported for the first time from Kerala and Western Ghats with a new host record, and Doryctus sp. (Braconidae is reported here.

  10. Levantamento da fauna de Coleoptera (Insecta associada à carcaça de roedores na região Sul do Brasil

    Directory of Open Access Journals (Sweden)

    Vinícius Costa-Silva

    2017-12-01

    Abstract. Coleoptera (Arthropoda, Insecta is considered one of the most important organism groups associated with organic matter decomposition and therefore may be useful to elucidate issues in the criminal context. The richness and abundance of beetles, including the necrophagous species, may vary according to climatic and physiogeographic conditions in different regions, thus the knowledge of the local entomofauna becomes relevant. Thereby, this study aimed to survey the local fauna and register the seasonal behavior of Coleoptera species associated with rodent carcasses exposed in a rural environment at Santa Maria, Rio Grande do Sul, Brazil (29°43'02.88"S 53°43'52.24"W. The collections were carried out quarterly throughout 12 months. Four Rattus norvegicus (Berkenhout carcasses weighing approximately 400 g were exposed, simultaneously, in each season, protected by a steel cage. Four pitfall traps were arranged around each carcass. A total of 1,856 specimens belonging to 14 families of Coleoptera were collected. The greatest abundance was observed during spring (N= 1,006, followed by summer (N= 518, winter (N= 319 and fall (N= 26. Records of the necrophagous entomofauna of Rio Grande do Sul are still scarce. Beyond contributing to the database promotion of the necrophagous species of beetle as a forensic purpose, it is expected that this paper may instigate the achievement of more faunistic surveys, regarding the biodiversity matter of two singular biomes present in the South region, Pampa and Atlantic Forest.Â

  11. Morphological and isozymic banding pattern study of white grubs (Coleoptera: Melolonthidae as pest of bark crop in mounth Merapi’s slope.

    Directory of Open Access Journals (Sweden)

    SUGIYARTO

    2008-07-01

    Full Text Available White grub (Coleoptera: Melolonthidae is a group of soil pest at any agrosystem., especially at Salak pondoh (Salacca zalacca (Gaert. Voss. crop. The characteristics of this specimen were very crucial to be studied in order to find the exact biocontrol. The aim of this research was to know the characteristics of white grubs (Melolonthidae: Coleoptera based on morphological and isozyme banding patterns. This research was conducted on August - November 2007 at Sleman and Magelang districts for the morphological purposes, while for the isozyme data were conducted at Sub Laboratory Biology, Central Laboratory of Sebelas Maret University Surakarta. Sample was taken by using stratified random sampling method, on five stations. Polyacrylamide gel electrophoresis (PAGE using the vertical type was taken to isozyme analysis. The enzyme used in this research were peroxidase and esterase to detect the isozyme banding patterns. The results showed that there was no morphological variation of white grubs (Melolonthidae: Coleoptera at salak pondoh agroecosystem in Mounth Merapi’s slope. Based on this character, there was one species of white grub found, i.e. Holotrichia javana. There was a genetic variation based on the variation of isozyme banding patterns.

  12. Uji Aktivitas Ekstrak Daun Seledri ( Apium graveolens L. terhadap Kumbang Kacang Callosobruchus chinensis L. (Coleoptera:Bruchidae

    Directory of Open Access Journals (Sweden)

    NI NENGAH DARMIATI

    2015-09-01

    Full Text Available The Activity Test of Celery Leaf Extract (Apium graveolens L. Against Bean Weevil, Callosobruchus cinensis L. (Coleoptera:Bruchidae The experiment was conducted at the Laboratory of Plant Pest and Disease Management,Department of Agroecotechnology, Faculty of Agriculture, Udayana University. The purpose of this experiment was to examine the activity of celery leaf extract against bean weevil, Callosobruchus chinensis L. The experiment was Randomized Complete Design, with five treatments of formulation concentration. The activities of celery leaf extract was indicated through i.e. contact poison test, repellent test, and the placement of eggs (oviposition test. The results showed that the celery leaf extract has activity as a contact poison with concentration 75% formulations caused over 50% death of the total insect. The extract with 100% concentration acted as a repellent and anti oviposition as well.

  13. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    International Nuclear Information System (INIS)

    Hoennicke, M.G.; Cusatis, C.; Rigon, L.; Menk, R.-H.; Arfelli, F.; Foerster, L.A.; Rosado-Neto, G.H.

    2010-01-01

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures (Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  14. Seasonal shifts in accumulation of glycerol biosynthetic gene transcripts in mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae, larvae

    Directory of Open Access Journals (Sweden)

    Jordie D. Fraser

    2017-06-01

    Full Text Available Winter mortality is a major factor regulating population size of the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae. Glycerol is the major cryoprotectant in this freeze intolerant insect. We report findings from a gene expression study on an overwintering mountain pine beetle population over the course of 35 weeks. mRNA transcript levels suggest glycerol production in the mountain pine beetle occurs through glycogenolytic, gluconeogenic and potentially glyceroneogenic pathways, but not from metabolism of lipids. A two-week lag period between fall glycogen phosphorylase transcript and phosphoenolpyruvate carboxykinase transcript up-regulation suggests that gluconeogenesis serves as a secondary glycerol-production process, subsequent to exhaustion of the primary glycogenolytic source. These results provide a first look at the details of seasonal gene expression related to the production of glycerol in the mountain pine beetle.

  15. Toxicities of azadirachtin and polychlorinated petroleum Hydrocarbon against resist and susceptible strains of tribolium castaneum (coleoptera: tenebrionidae) adults

    International Nuclear Information System (INIS)

    Hussain, R.; Riaz, M.; Riaz, M.

    2014-01-01

    The LC/sub 50/ values for malathion-resistant (PAK) and organo-50 phosphate-susceptible (FSS-II) strains of red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) adult beetles were determined through filter paper impregnated method against azadirachtin (Nimbokil 60 EC) and polychlorinated petroleum hydrocarbon (Tenekil 100 EC). The LC values of these insecticides were worked out as 12830 and 50 9331 ppm for azadirachtin and 5148 and 4047 ppm for Tenekil 100 EC against PAK and FSS-II strains, respectively. The results revealed that polychlorinated petroleum hydrocarbon was more toxic than the azadirachtin. Furthermore, both the insecticides were equally toxic to the adult beetles of T. castaneum as the difference was non-significant because of overlapping 95% FLs to LC./sub 50/. (author)

  16. Two new species of the megadiverse lentic diving-beetle genus Hydrovatus (Coleoptera, Dytiscidae described from NE Thailand

    Directory of Open Access Journals (Sweden)

    Olof Bistrom

    2016-11-01

    Full Text Available Here we describe two new Hydrovatus species (Coleoptera: Dytiscidae: Hydroporinae: Hydrovatini from the province of Khon Kaen, Isan region in NE Thailand. Hydrovatus is the third most species rich genus of diving beetles (Dytiscidae. It occurs on all continents except Antarctica and now numbers 210 currently recognized species. Both new species, H. diversipunctatus sp. n. and H. globosus sp. n., were collected at lights and are only known from the type locality “Khon Kaen” (a city and province. Diagnoses based on morphology for the separation from closely related species are given together with illustrations of male genitalia and habitus photos. We provide a determination key to Old World species of the pustulatus species group and to Oriental species of the oblongipennis species group.

  17. Taxonomic notes on the ground beetles in the genus Trephionus Bates, 1883 from central Honshu, Japan (Coleoptera, Carabidae, Sphodrini, Synuchina).

    Science.gov (United States)

    Sasakawa, Kôji; Itô, Hirotarô

    2018-01-01

    Trephionus Bates, 1883, a Japanese endemic genus in the subtribe Synuchina (Coleoptera, Carabidae, Sphodrini), is revised taxonomically based mainly on the shape of the endophallus, a membranous inner sac everted from the aedeagus of the male genitalia. Three known species from central Honshu, T. kinoshitai Habu, 1954; T. shibataianus Habu, 1978; and T. babai Habu, 1978, are re-defined based on this genital character, and five new species are described from the region: T. cylindriphallus Sasakawa, sp . n ., T. niumontanus Sasakawa, sp . n ., T. inexpectatus Sasakawa & Itô, sp . n ., T. abiba Sasakawa & Itô, sp . n ., and T. bifidilobatus Sasakawa & Itô, sp . n . The observed interspecies differences in endophallus morphology are discussed in terms of the species-level phylogeny and genus-level taxonomy of Trephionus .

  18. Taxonomic notes on the ground beetles in the genus Trephionus Bates, 1883 from central Honshu, Japan (Coleoptera, Carabidae, Sphodrini, Synuchina

    Directory of Open Access Journals (Sweden)

    Kôji Sasakawa

    2018-03-01

    Full Text Available Trephionus Bates, 1883, a Japanese endemic genus in the subtribe Synuchina (Coleoptera, Carabidae, Sphodrini, is revised taxonomically based mainly on the shape of the endophallus, a membranous inner sac everted from the aedeagus of the male genitalia. Three known species from central Honshu, T. kinoshitai Habu, 1954; T. shibataianus Habu, 1978; and T. babai Habu, 1978, are re-defined based on this genital character, and five new species are described from the region: T. cylindriphallus Sasakawa, sp. n., T. niumontanus Sasakawa, sp. n., T. inexpectatus Sasakawa & Itô, sp. n., T. abiba Sasakawa & Itô, sp. n., and T. bifidilobatus Sasakawa & Itô, sp. n. The observed interspecies differences in endophallus morphology are discussed in terms of the species-level phylogeny and genus-level taxonomy of Trephionus.

  19. The collection of type specimens of the family Carabidae (Coleoptera deposited in the Natural History Museum of Barcelona, Spain

    Directory of Open Access Journals (Sweden)

    Viñolas, A.

    2014-05-01

    Full Text Available The type collection of the family Carabidae (Coleoptera deposited in the Natural History Museum of Barcelona, Spain, has been organised, revised and documented. It contains 430 type specimens belonging to 155 different taxa. Of note are the large number of hypogean species, the species of Cicindelidae from Asenci Codina’s collection, and the species of Harpalinae extracted from Jacques Nègre’s collection. In this paper we provide all the available information related to these type specimens. We therefore provide the following information for each taxon, species or subspecies: the original and current taxonomic status, original citation of type materials, exact transcription of original labels, and preservation condition of specimens. Moreover, the differences between original descriptions and labels are discussed. When a taxonomic change has occurred, the references that examine those changes are included at the end of the taxa description.

  20. Variation in diel activity of ground beetles (Coleoptera: Carabidae) associated with a soybean field and coal mine remnant

    Science.gov (United States)

    Willand, J.E.; McCravy, K.W.

    2006-01-01

    Diel activities of carabids (Coleoptera: Carabidae) associated with a coal mine remnant and surrounding soybean field were studied in west-central Illinois from June through October 2002. A total of 1,402 carabids, representing 29 species and 17 genera, were collected using pitfall traps. Poecilus chalcites (Say) demonstrated roughly equal diurnal and nocturnal activity in June, but greater diurnal activity thereafter. Pterostichus permundus (Say), Cyclotrachelus seximpressus (LeConte), Amara obesa (Say), and Scarites quadriceps Chaudoir showed significant nocturnal activity. Associations between habitat and diel activity were found for three species: P. chalcites associated with the remnant and edge habitats showed greater diurnal activity than those associated with the soybean field; C. seximpressus was most active diurnally in the remnant, and Harpalus pensylvanicus (DeGeer) showed the greatest nocturnal activity in the remnant and edge habitats. We found significant temporal and habitat-related variation in diel activity among carabid species inhabiting agricultural areas in west-central Illinois.

  1. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.

  2. Parasitylenchus bifurcatus n. sp. (Tylenchida: Allantonematidae parasitizing Harmonia axyridis (Coleoptera: Coccinellidae

    Directory of Open Access Journals (Sweden)

    Poinar George O

    2012-10-01

    Full Text Available Abstract Background The harlequin ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae is native to central and eastern Asia and was purposely introduced into Europe to control aphids. While it proved to be a good biological control agent, its rapid spread and buildup of large populations made it a nuisance, since it overwinters in homes, emits unpleasant odors, stains fabrics, occasionally bites humans and feeds on apples, pears and grapes. Aside from the above, the ravenous appetite of H. axyridis results in their consumption of harmless native insects, including even other ladybird beetles. A study of the natural enemies of H. axyridis in Denmark revealed the presence of nematodes. The present study describes this nematode parasite and discusses aspects of its development and ecology. Methods Adult harlequin ladybird beetles were collected from March to November from four localities in Copenhagen on different plant species. In addition, groups of last-instar larvae and pupae (n = 50 were examined for the presence of nematodes. Living and recently dead nematodes were removed from adult H. axyridis in 0.5% saline solution, the nematodes were then heat killed (at 75C, fixed in 5% formalin and transferred to glycerin on slides for further examination and measurements. Results A new species of Allantonematidae (Tylenchida, Parasitylenchus bifurcatus n. sp., is described from adults of the harlequin ladybird, Harmonia axyridis in Denmark. The new species is characterized by a straight stylet lacking basal thickenings, a bursa and a forked tail tip in the vermiform (infective females and juvenile males. The new species is compared with P. coccinellinae previously described from ladybird beetles in France. Parasitism resulted in depletion of the fat body and partial or complete atrophy of the reproductive organs of the beetles. Infections occurred throughout the year with rates of parasitism reaching up to 35%. The rate increased to 60

  3. SINOPSIS DE LOS ESCARABAJOS COPRÓFAGOS (COLEOPTERA: SCARABAEINAE DEL CARIBE COLOMBIANO

    Directory of Open Access Journals (Sweden)

    JORGE ARI NORIEGA

    2013-12-01

    Full Text Available La subfamilia Scarabaeinae (Coleoptera: Scarabaeidae es un grupo de gran importancia en los ecosistemas por su significativa participación en el proceso de reciclaje de nutrientes. Para Colombia existen algunos acercamientos al análisis de su diversidad en ciertas regiones biogeográficas. Sin embargo, existen zonas que necesitan un mayor estudio, como es el caso de la región Caribe. Con el fin de llenar el vacío de información se realizó una revisión de la información depositada en 16 colecciones a nivel nacional y de la literatura en esta zona. Se registran 90 especies pertenecientes a 24 géneros, agrupadas en seis tribus. Se reportan 20 nuevas especies para el departamento de La Guajira, 18 para Sucre, once para Magdalena, nueve para Bolívar, nueve para Córdoba, tres para Atlántico y dos para Cesar. El departamento con la mayor riqueza es Magdalena ya que es el mejor muestreado incluyendo su rango altitudinal. Al analizar la composición de especies, la región Caribe presenta pocos endemismos, exceptuando las zonas altas del Macizo de la Sierra Nevada de Santa Marta. El género Onthophagus es el más diverso en la región y la especie que presenta el mayor rango altitudinal es Ontherus sanctaemartae (700 hasta 2500 m . La región es homogénea en las zonas bajas, presentándose una mayor afinidad con el Chocó biogeográfico. Se establecen en la región cuatro grandes franjas altitudinales: planicies (0- 500 m , colinas altas (>500- 900 m , zona subandina-andina (> 900 a 2500 m y alta montaña (> 2500 m . Es posible que con el incremento de muestreos en zonas poco estudiadas la homogeneidad específica aumente. Finalmente, es necesario ampliar e intensificar los estudios en esta región, especialmente en los sistemas montañosos aislados.

  4. Bazı Biyolojik Preparatların Sitophilus granarius (Coleoptera: Curculionidae Erginlerine Etkileri

    Directory of Open Access Journals (Sweden)

    Tuğba AYYILDIZ

    2017-09-01

    Full Text Available Bu çalışmada, Entomopatojen funguslardan Biocatch, Nibortem (Verticillium lecani, BMAUM-M3-003 (Metharizum anisopliae ile okaliptus ve zencefil bitkilerinden elde edilen uçucu yağların ve bir bitkisel ekstrakt preparatı olan Nibedicine EC (Azadiractin’in buğdaylarda oldukça önemli zarara neden olan Sitophilus granarius (Coleoptera: Curculionidae erginlerine karşı laboratuar koşullarında etkinlikleri denenmiştir. 10x10x8 cm boyutlarında ağızları tül ile yapıştırılmış olan plastik kaplarda ve 25±1 °C sıcaklık, %60±5 nem oranlarında ve 16:8 saat ışıklandırma süresine ayarlı iklim odasında yetiştirilen S. granarius erginleri üzerine Biocatch, Nibortem (Verticillium lecanii entomopatojen funguslardan 1x109  cfu/mL, BMAUM-M3-003 (Metharizum anisopliae 1x108  cfu/mL dozunda, Nibedicine EC (Azadirachtin 3000 ppm dozunda, uçucu yağlar ise %1 (1 l/100 l dozunda uygulanmıştır. Çalışma sonucunda elde edilen verilere göre BMAUM-M3-003 kodlu entomopatojen fungus (M. anisopliae, S. granarius’u zamana bağlı olarak en fazla etkileyen etmen olmuştur. Yapılan değerlendirmeler sonucunda kullanılan preparatların 21 gün sonunda etkileri incelendiğinde, zencefilin %89.47, diğerlerinin ise %100 ölüm oranına sahip olduğu saptanmıştır.

  5. Species delimitation in northern European water scavenger beetles of the genus Hydrobius (Coleoptera, Hydrophilidae)

    Science.gov (United States)

    Fossen, Erlend I.; Ekrem, Torbjørn; Nilsson, Anders N.; Bergsten, Johannes

    2016-01-01

    Abstract The chiefly Holarctic Hydrobius species complex (Coleoptera, Hydrophilidae) currently consists of Hydrobius arcticus Kuwert, 1890, and three morphological variants of Hydrobius fuscipes (Linnaeus, 1758): var. fuscipes, var. rottenbergii and var. subrotundus in northern Europe. Here molecular and morphological data are used to test the species boundaries in this species complex. Three gene segments (COI, H3 and ITS2) were sequenced and analyzed with Bayesian methods to infer phylogenetic relationships. The Generalized Mixed Yule Coalescent (GMYC) model and two versions of the Bayesian species delimitation method BPP, with or without an a priori defined guide tree (v2.2 & v3.0), were used to evaluate species limits. External and male genital characters of primarily Fennoscandian specimens were measured and statistically analyzed to test for significant differences in quantitative morphological characters. The four morphotypes formed separate genetic clusters on gene trees and were delimited as separate species by GMYC and by both versions of BPP, despite specimens of Hydrobius fuscipes var. fuscipes and Hydrobius fuscipes var. subrotundus being sympatric. Hydrobius arcticus and Hydrobius fuscipes var. rottenbergii could only be separated genetically with ITS2, and were delimited statistically with GMYC on ITS2 and with BPP on the combined data. In addition, six or seven potentially cryptic species of the Hydrobius fuscipes complex from regions outside northern Europe were delimited genetically. Although some overlap was found, the mean values of six male genital characters were significantly different between the morphotypes (p < 0.001). Morphological characters previously presumed to be diagnostic were less reliable to separate Hydrobius fuscipes var. fuscipes from Hydrobius fuscipes var. subrotundus, but characters in the literature for Hydrobius arcticus and Hydrobius fuscipes var. rottenbergii were diagnostic. Overall, morphological and molecular

  6. Evaluation of double-decker traps for emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Poland, Therese M; McCullough, Deborah G; Anulewicz, Andrea C

    2011-04-01

    Improved detection tools are needed for the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive forest insect from Asia that has killed millions of ash (Fraxinus spp.) trees in North America since its discovery in Michigan in 2002. We evaluated attraction of adult A. planipennis to artificial traps incorporating visual (e.g., height, color, silhouette) and olfactory cues (e.g., host volatiles) at field sites in Michigan. We developed a double-decker trap consisting of a 3-m-tall polyvinyl pipe with two purple prisms attached near the top. In 2006, we compared A. planipennis attraction to double-decker traps baited with various combinations of manuka oil (containing sesquiterpenes present in ash bark), a blend of four ash leaf volatiles (leaf blend), and a rough texture to simulate bark. Significantly more A. planipennis were captured per trap when traps without the rough texture were baited with the leaf blend and manuka oil lures than on traps with texture and manuka oil but no leaf blend. In 2007, we also tested single prism traps set 1.5 m above ground and tower traps, similar to double-decker traps but 6 m tall. Double-decker traps baited with the leaf blend and manuka oil, with or without the addition of ash leaf and bark extracts, captured significantly more A. planipennis than similarly baited single prism traps, tower traps, or unbaited double-decker traps. A baited double-decker trap captured A. planipennis at a field site that was not previously known to be infested, representing the first detection event using artificial traps and lures. In 2008, we compared purple or green double-decker traps, single prisms suspended 3-5 m above ground in the ash canopy (canopy traps), and large flat purple traps (billboard traps). Significantly more A. planipennis were captured in purple versus green traps, baited traps versus unbaited traps, and double-decker versus canopy traps, whereas billboard traps were intermediate. At sites

  7. Karyotypes of some medium-sized Dytiscidae (Agabinae and Colymbetinae (Coleoptera

    Directory of Open Access Journals (Sweden)

    Robert Angus

    2013-06-01

    Full Text Available An account is given of the karyotypes of 29 species of medium sized Dytiscidae (Coleoptera. Of the 20 species of Agabus Leach, 1817, 18 have karyotypes comprising 21 pairs of autosomes and sex chromosomes which are either X0(♂ or XX (♀. These species are A. serricornis (Paykull, 1799, A. labiatus (Brahm, 1791, A. congener (Thunberg, 1794, A. lapponicus (Thomson, 1867, A. thomsoni (J. Sahlberg, 1871, A. confinis (Gyllenhal, 1808, A. sturmii (Gyllenhal, 1808, A. bipustulatus (Linnaeus, 1767, A. nevadensis Håkan Lindberg, 1939, A. wollastoni Sharp, 1882, A. melanarius Aubé, 1837, A. biguttatus (Olivier, 1795, A. binotatus Aubé, 1837, A. affinis (Paykull, 1798, A. unguicularis (Thomson, 1867, A. ramblae Millan & Ribera, 2001, A. conspersus (Marsham, 1802 and A. nebulosus (Forster, 1771. However two species, A. infuscatus Aubé, 1838 and A. adpressus Aubé, 1837, have developed a neo-XY system, with karyotypes comprising 21 pairs of autosomes and XY sex chromosomes (♂. No chromosomal differences have been detected between typical A. bipustulatus and A. bipustulatus var. solieri Aubé, 1837, nor have any been found between the three species of the A. bipustulatus complex (A. bipustulatus, A. nevadensis and A. wollastoni. The four species of Colymbetes Clairville, 1806, C. fuscus (Linnaeus, 1758, C. paykulli Erichson, 1837, C. piceus Klug, 1834 and C. striatus (Linnaeus, 1758 have karyotypes comprising 20 pairs of autosomes and sex chromosomes which are X0 (♂, XX (♀. Two of the species of Rhantus Dejean, 1833, R. exsoletus (Forster, 1771 and R. suturellus (Harris, 1828 have karyotypes comprising 20 pairs of autosomes and X0/XX sex chromosomes, but the other three species, R. grapii (Gyllenhal, 1808, R. frontalis (Marsham, 1802 and R. suturalis (Macleay, 1825 have 22 pairs of autosomes and X0/XX sex chromosomes. Agabus congener and Rhantus suturellus may have one B-chromosome. Nine of the species have previously published karyotype data but

  8. Diversity and altitudinal distribution of Chrysomelidae (Coleoptera in Peregrina Canyon, Tamaulipas, Mexico

    Directory of Open Access Journals (Sweden)

    Uriel Jeshua Sánchez-Reyes

    2014-06-01

    Full Text Available The Chrysomelidae (Coleoptera is a highly speciose family that has been poorly studied at the regional level in Mexico. In the present study, we estimated species richness and diversity in oak-pine forest, Tamaulipan thorny scrub and in tropical deciduous forests in Peregrina Canyon within the Altas Cumbres Protected Area of the northeastern state of Tamaulipas, Mexico. Sampling of Chrysomelidae consisted of five sweep net samples (200 net sweeps within each of three sites during four sample periods: early dry season, late dry season, early wet season, and late wet season. Species were identified and total numbers per species were recorded for each sample. A total of 2,226 specimens were collected belonging to six subfamilies, 81 genera and 157 species of Chrysomelidae from the study area. Galerucinae was the most abundant subfamily with 1,828 specimens, representing 82.1% of total abundance in the study area. Lower abundance was recorded in Cassidinae (8.5%, Eumolpinae (3.6%, Cryptocephalinae (2.2%, Chrysomelinae (2.2%, and finally Criocerinae (1.3%. The highest species richness was also presented in the subfamily Galerucinae with 49% of the total obtained species followed by Cassidinae (20%, Cryptocephalinae (9.7%, Eumolpinae (9.7%, Chrysomelinae (6.5% and Criocerinae (5.2%. The most common species were Centralaphthona fulvipennis Jacoby (412 individuals, Centralaphthona diversa (Baly (248, Margaridisa sp.1 (219, Acallepitrix sp.1 (134, Longitarsus sp.1 (104, Heterispa vinula (Erichson (91, Epitrix sp.1 (84 and Chaetocnema sp.1 (72. Twenty-two species were doubletons (1.97% of total abundance and 52 were singletons (2.33%. The estimated overall density value obtained was 0.0037 individuals/m2. The greatest abundance and density of individuals were recorded at the lowest elevation site. However, alpha diversity increased with increasing altitude. Similarity values were less than 50% among the three sites indicating that each site had distinct

  9. Coleoborers (Coleoptera: Cerambycidae, Curculionidae associated to culture wastes in mango crops in José de Freitas county – Piauí/ Coleobrocas (Coleoptera: Cerambycidae, Curculionidae associadas a restos culturais da cultura da manga (Mangifera indica L. – Anacardiaceae no município de José de Freitas – Piauí

    Directory of Open Access Journals (Sweden)

    Sávio Silveira Feitosa

    2007-08-01

    Full Text Available The coleoborers use the wood to complete part of their cycle of life. In search for food they can make galleries and cause damages in the structure of the plant, allowing the entrance of pathogenic individuals that will harm the development of the crop. This research was carried out from January to May 2005 aiming to get information on the species of coleoborers associated to crop wastes in the area of mango crop, in José de Freitas county - Piauí. The insects had been collected in areas of Tommy Atkins, Keitt, Kent and Palmer varieties, in 10 fallen branches, with approximately 50 cm long and 2.5 cm of diameter, that were taken to the plant health laboratory of Centro de Ciências Agrárias in Universidade Federal do Piauí. The coleoborers were removed from the wood, assembled in entomologic pins and sent to the Instituto Biológico in São Paulo SP for species identification. The coleoborers Hypothenemus sp. (Coleoptera: Curculionidae: Scolytinae, Xyleborus sp. (Coleoptera: Curculionidae: Scolytinae and Orthostoma chryseis (Bates, 1970 (Coleoptera: Cerambycidae were found.As coleobrocas usam a madeira para realizar o seu ciclo de vida, fazendo galerias e causando danos na estrutura da planta e em certas situações, permitindo a entrada de patógenos. Este trabalho foi realizado de janeiro a maio de 2005 objetivando identificar e obter informações sobre as espécies de coleobrocas associadas a restos de cultura em área de cultivo de manga, no município de José de Freitas-Piauí. Foram coletados em cada área das variedades Tommy Atkins, Keitt, Kent e Palmer, dez ramos caídos, com aproximadamente 50 cm de comprimento e 2,5 cm de diâmetro, que foram levados ao laboratório de Fitossanidade do Centro de Ciências Agrárias da Universidade Federal do Piauí. As coleobrocas adultas foram retiradas da madeira, montadas em alfinetes entomológicos e enviadas ao Instituto Biológico em São Paulo-SP para identificação das espécies. Foram

  10. Associação forética entre larvas de Rheotanytarsus (Chironomidae, Tanytarsini e adultos de Elmidae (Coleoptera, coletados em córregos no Parque Estadual de Campos do Jordão, São Paulo, Brasil Phoretic association between larvae of Rheotanytarsus and adult of Coleoptera, collected from State Park Campos do Jordão, São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    Melissa O. Segura

    2007-06-01

    Full Text Available Esta nota registra a ocorrência de foresia entre larvas de Rheotanytarsus Thienemann & Bause, 1913 (Chironomidae, Tanytarsini e adultos de Hexacylloepus Hinton, 1940 e Microcylloepus Hinton, 1935 (Coleoptera, Elmidae. Os exemplares foram obtidos em córregos no Parque Estadual de Campos de Jordão, Estado de São Paulo, Brasil.This note reports phoresy between larvae of Rheotanytarsus Thienemann & Bause, 1913 (Chironominae, Tanytarsini and adult of Hexacylloepus Hinton, 1940 and Microcylloepus Hinton, 1935 (Coleoptera, Elmidae. The specimens had been collected in streams from State Park Campos do Jordão, São Paulo State, Brazil.

  11. Levantamento da fauna de Coleoptera que habita a carcaça de Sus scrofa L., em Curitiba, Paraná A study of the Coleoptera (Insecta fauna that inhabits Sus scrofa L. carcass in Curitiba, Paraná

    Directory of Open Access Journals (Sweden)

    Kleber Makoto Mise

    2007-09-01

    Full Text Available Este trabalho visou levantar a fauna de Coleoptera associada à carcaça de Sus scrofa L.,1758, espécie utilizada como modelo em Entomologia Forense. O acréscimo ou a substituição seqüencial das espécies de insetos pode ser utilizado para estimar o intervalo post mortem (IPM. O experimento foi realizado no Centro Politécnico (UFPR, de setembro de 2005 a setembro de 2006. A cada estação foi sacrificado um suíno de 15 kg no local, colocado em gaiola. A captura dos insetos foi realizada diariamente em bandeja posicionada abaixo da carcaça e em armadilha tipo Shannon modificada, e a cada 14 dias em cinco armadilhas do tipo pit-fall. Foram coletados 4.360 Coleoptera, pertencentes a 112 espécies de 26 famílias, 12 consideradas de importância forense. A coleta ativa realizada na bandeja foi responsável pela maior captura (2.023 espécimes, seguida pela armadilha Shannon modificada (2.016 espécimes e por último pelas do tipo pit-fall (324 espécimes. Staphylinidae foi mais coletada na bandeja e Shannon modificada, e Silphidae na armadilha pit-fall. Os principais hábitos encontrados foram predador/parasita (55%e onívoro (38,05%, com poucas espécies consideradas necrófagas (1,31%.This paper sought to assess the Coleoptera fauna associated with carcasses of Sus scrofa L., 1758, which is usually used as model in Forensic Entomology. The addition and sequential substitution of insect species could be used to estimate the post mortem interval (PMI. The present study took place in Centro Politécnico (UFPR, between september 2005 to september 2006. A pig weighting 15 kg was sacrificed each season and put inside a cage. Sampling is made daily in a tray placed below the carcass and in a Shannon modified trap, and each 14 days in five pit-fall traps. 4,360 beetles were collected, belonging to 112 species of 26 families, 12 were considered of forensic potential. The active collecting made in the tray was responsible for the largest number of

  12. Türkiye’nin Farklı Coğrafik Bölgelerinden Toplanan Propolis Örneklerinin Aspergillus versicolor ve Penicillium aurantiogriseum’e Karşı Antifungal Aktiviteleri (İngilizce

    Directory of Open Access Journals (Sweden)

    Ayhan Temiz

    2015-02-01

    Full Text Available Bu çalışmanın amacı, Türkiye’nin çeşitli coğrafik bölgelerinden toplanan 10 propolis örneğinin mikotoksin üreticisi olan, gıda kaynaklı iki küf (Aspergillus versicolor ve Penicillium aurantiogriseum suşuna karşı antifungal aktivitelerini araştırmaktır. Propolis örneklerinin etil alkol ekstraktlarının (EEP kimyasal kompozisyonları kütle spektorometresine birleştirilmiş gaz kromatografisi ile belirlenmiştir. EEP örneklerinin antifungal aktiviteleri %1, %5 ve %10 olacak şekilde üç farklı konsantrasyonda test edilmiştir. EEP örneklerinin antifungal aktiviteleri, incelenen küf suşunun Patates Dekstroz Agar besiyeri yüzeyindeki misel gelişiminde meydana gelen inhibisyonun yüzdesi şeklinde belirlenmiştir. EEP örneklerinin ana bileşenleri flavonoitler, aromatik alkoller, aromatik asit esterler, alifatik asit esterler, aromatik asitler, alkoller ve aromatik hidrokarbonlar olarak tespit edilmiştir. EEP örneklerinin tümü %10 konsantrasyonda her iki küf suşunun misel gelişimi üzerinde %100 inhibisyon sağlamıştır. EEP örneklerinin iki küf suşu üzerindeki antifungal etkisi %1 ve %5 konsantrasyonlarında değişkenlik göstermiştir. Araştırma sonuçları, P. aurantiogriseum suşunun misel gelişimi üzerinde inhibisyon etkisi yaratması için A. versicolor’a kıyasla daha yüksek bir EEP konsantrasyonuna gereksinim olduğuna işaret etmektedir. Küf suşları üzerinde etkin olan propolis örneklerinin flavonoit yüzdesi diğer bileşenlerin yüzdesinden oldukça daha yüksektir. Bu sonuçlar, propolis örneklerinin EEP konsantrasyonuna ve kimyasal kompozisyonuna bağlı olarak iki küf suşu üzerinde de belirgin kuvvette bir antifungal etki yarattığına işaret etmektedir.

  13. Macrodactylini (Coleoptera, Scarabaeidae, Melolonthinae: primary types of type species and taxonomic changes to the generic classification

    Directory of Open Access Journals (Sweden)

    Juares Fuhrmann

    2017-09-01

    Full Text Available Type series for 35 type species of Macrodactylini (Coleoptera: Scarabaeidae: Melolonthinae are studied and taxonomic changes are proposed. The following 35 lectotypes are designated: Agaocnemis pruina Moser, 1918; Amphicrania ursina Burmeister, 1855; Anomalochilus singularis Blanchard, 1850; Anomalonyx uruguayensis Moser, 1921; Aulanota sulcipennis Moser, 1924; Barybas nanus Blanchard, 1850; Barybas volvulus Burmeister, 1855; Calodactylus tibialis Blanchard, 1850; Ceraspis pruinosa LePeletier de Saint-Fargeau & Audinet-Serville, 1828; Ceratolontha venezuelae Arrow, 1948; Chariodactylus chacoensis Moser, 1919; Clavipalpus dejeani Laporte, 1832; Corminus canescens Burmeister, 1855; Ctenotis obesa Burmeister, 1855; Ctilocephala pellucens Burmeister, 1855; Demodema fallax Blanchard, 1850; Euryaspis gaudichaudii Blanchard, 1851; Faula cornuta Blanchard, 1850; Gama grandicornis Blanchard, 1850; Gastrohoplus mirabilis Moser, 1921; Mallotarsus spadiceus Blanchard, 1850; Manodactylus gaujoni Moser, 1919; Manopus biguttatus Conte de Castelnau, 1840; Melolontha rufipennis Fabricius, 1801; Oedichira pachydactyla Burmeister, 1855; Pachycerus castaneipennis Guérin-Méneville, 1831; Pachylotoma viridis Blanchard, 1850; Pectinosoma elongata Arrow, 1913; Philochlaenia virescens Blanchard, 1842; Plectris tomentosa LePeletier de Saint-Fargeau & Audinet-Serville, 1828; Pseudohercitis viridiaenea Moser, 1921; Rhinaspoides aeneofusca Moser, 1919; Schizochelus flavescens Blanchard, 1850; Serica marmorea Guérin-Méneville, 1831; and Ulomenes hypocrita Blanchard, 1850. The following six genera are revalidated: Byrasba Harold, 1869 (formerly a synonym of Rhinaspis Perty, 1833; Euryaspis Blanchard, 1851 (formerly a synonym of Plectris LePeletier de Saint-Fargeau & Audinet-Serville, 1828; Junkia Dalla Torre, 1913 (formerly a synonym of Plectris; Faula Blanchard, 1850 (formerly a synonym of Ceraspis LePeletier de Saint-Fargeau & Audinet-Serville, 1828; Paulosawaya

  14. Key to higher taxa of South American weevils based on adult characters (Coleoptera, Curculionoidea Clave de taxones superiores de gorgojos sudamericanos basada en caracteres de los adultos (Coleoptera, Curculionoidea

    Directory of Open Access Journals (Sweden)

    ADRIANA E. MARVALDI

    2005-03-01

    Full Text Available The weevils (Coleoptera: Curculionoidea from South America are currently classified in the following families and subfamilies: Nemonychidae (Rhinorhynchinae, Anthribidae (Anthribinae, Belidae (Belinae and Oxycoryninae, Attelabidae (Attelabinae and Rhynchitinae, Brentidae (Apioninae and Brentinae, Caridae (Carinae and Curculionidae (Erirhininae, Dryophthorinae, Entiminae, Aterpinae, Gonipterinae, Rhythirrininae, Thecesterninae, Eugnominae, Hyperinae, Curculioninae, Cryptorhynchinae, Mesoptiliinae (= Magdalidinae, Molytinae, Baridinae, Lixinae, Conoderinae (= Zygopinae, Cossoninae, Scolytinae and Platypodinae. In the present contribution we bring a dichotomous key for the identification of seven families and 28 subfamilies of Curculionoidea from South America, and for 21 tribes of the highly heterogeneous subfamilies Curculioninae and Molytinae. These tribes are Curculionini Anthonomini, Ceutorhynchini, Derelomini, Otidocephalini, Erodiscini, Camarotini, Piazorhinini, Prionobrachiini, Smicronychini, Rhamphini and Tychiini, within Curculioninae; and Hylobiini, Pissodini, Conotrachelini, Cleogonini, Sternechini, Pacholenini, Cholini, Petalochilini and Amalactini, within Molytinae. Most of them have been classified as subfamilies in traditional schemes. The key is mainly based on external morphological characters, but also includes data on genitalia, mouth parts and other biological features. Definitions and illustrations of diagnostic characters used in the key are providedLos gorgojos (Coleoptera: Curculionoidea de América del Sur se clasifican actualmente en las siguientes familias y subfamilias: Nemonychidae (Rhinorhynchinae, Anthribidae (Anthribinae, Belidae (Belinae y Oxycoryninae, Attelabidae (Attelabinae y Rhynchitinae, Brentidae (Apioninae y Brentinae, Caridae (Carinae y Curculionidae (Erirhininae, Dryophthorinae, Entiminae, Aterpinae, Gonipterinae, Rhythirrininae, Thecesterninae, Eugnominae, Hyperinae, Curculioninae, Cryptorhynchinae

  15. A new species of Pacholenus Schoenherr from southeastern Brazil (Coleoptera, Curculionidae, Molytinae, and new occurrences of species of the genus

    Directory of Open Access Journals (Sweden)

    Sergio Antonio Vanin

    2008-01-01

    Full Text Available A new species of Pacholenus Schoenherr from southeastern Brazil (Coleoptera, Curculionidae, Molytinae, and new occurrences of species of the genus. Pacholenus monteiroi sp. nov. (type-locality Brazil, Rio de Janeiro state, Carapebus, Parque Nacional da Restinga de Jurubatiba is described and illustrated. The weevil develops as a stem gall-former in Calyptranthes brasiliensis Spreng (Myrtaceae. The new species is easily distinguished from the other five known of the genus by the presence of a prominent supra-ocular ridge. An updated key for identification for all species of Pacholenus is provided. Three species of Pacholenus are presently recorded for the states of Rio de Janeiro and São Paulo; P. pelliceus and P. monteiroi occur in both states, while P. penicillus is only known from Rio de Janeiro; P. hispidus occurs in São Paulo, being the most widespread species of the genus, ranging from Minas Gerais south to Santa Catarina.Nova espécie de Pacholenus Schoenherr do sudeste do Brasil (Coleoptera, Curculionidae, Molytinae, e novas ocorrências de espécies do gênero. Pacholenus monteiroi sp. nov. (localidade-tipo Brasil, Estado do Rio de Janeiro, Carapebus, Parque Nacional da Restinga de Jurubatiba é descrita e ilustrada. Esse gorgulho se desenvolve em galhas caulinares de Calyptranthes brasiliensis Spreng (Myrtaceae. A nova espécie é facilmente distinta das outras cinco conhecidas do gênero devido à presença de uma crista supra-ocular proeminente. Uma chave atualizada para identificação das espécies de Pacholenus é fornecida. Três espécies de Pacholenus ocorrem nos estado do Rio de Janeiro e São Paulo; P. pelliceus e P. monteiroi ocorrem em ambos os estados, enquanto que P. penicillus apenas no Rio de Janeiro; P. hispidus ocorre em São Paulo, sendo a espécie do gênero com a distribuição mais ampla, sendo encontrada desde Minas Gerais até Santa Catarina.

  16. Response to host volatiles by native and introduced populations of Dendroctonus valens (Coleoptera: Curculionidae, Scolytinae) in North America and China.  Journal of Chemical Ecology 33: 131-146.

    Science.gov (United States)

    N. Erbilgin; S.R. Mori; J.H. Sun; J.D. Stein; D.R. Owen; L.D. Merrill; R. Campos Bolande; os; K.F. Raffa; T. Mendez Montiel; D.L. Wood; N.E.  Gillette

    2007-01-01

    Bark beetles (Coleoptera: Curculionidae, Scolytinae) have specialized feeding habits, and commonly colonize only one or a few closely related host genera in their geographical ranges. The red turpentine beetle, Dendroctonus valens LeConte, has a broad geographic distribution in North America and exploits volatile cues from a wide variety of pines...

  17. Efecto de la dieta artificial MP sobre la emergencia y relacion de sexos de Phymastichus coffea (Hymenoptera:Eulophidae) mantenido sobre su hueped, Hypothenemus hampei (Coleoptera: Scloytidae)a traves de generaciones contin

    Science.gov (United States)

    Phymastichus coffea La Salle (Hymenoptera: Eulophidae) is an endoparasitoid that attacks the adult coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Scolytidae). The MP diet developed by Portilla and Streett is the only reported diet that allows cultures of P. coffea to develop and repr...

  18. Preharvest quarantine treatments of Chlorantraniliprole,Clothianidin, & Imidacloprid-based insecticides for control of Japanese beetle Coleoptera:Scarabaeidae)& other scarab larvae in the root zone of field-grown nurserytrees

    Science.gov (United States)

    Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), is an important quarantine pest of nurseries. Nursery plant movement from P. japonica-infested regions is regulated by the U.S. Domestic Japanese Beetle Harmonization Plan (DJHP), which classifies states by risk categories. Treatm...

  19. Effectiveness of bifenthrin (Onyx™) and carbaryl (Sevin® SL) for protecting individual, high-value trees from bark beetle attack (Coleoptera: Curculionidae: Scolytinae) in the western United States

    Science.gov (United States)

    Christopher J. Fettig; Kurt K. Allen; Robert R. Borys; John Christopherson; Christopher P. Dabney; Thomas J. Eager; Kenneth E. Gibson; Elizabeth G. Hebertson; Daniel F. Long; A. Steven Munson; Patrick J. Shea; Sheri L. Smith; Michael I. Haverty

    2006-01-01

    High-value trees, such as those located in residential, recreational, or administrative sites, are particularly susceptible to bark beetle (Coleoptera: Curculionidae: Scolytinae) attack as a result of increased amounts of stress associated with drought, soil compaction, mechanical injury, or vandalism. Tree losses in these unique environments generally have a...

  20. Long-term monitoring of the introduced emerald ash borer (Coleoptera: Buprestidae) egg parasitoid, oobius agrili (Hymenoptera: Encyridae), in Michigan, USA and evaluation of a newly developed monitoring technique

    Science.gov (United States)

    Emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a serious invasive pest of ash trees (Fraxinus spp.) in North America. The egg parasitoid Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae) was introduced as a biological control agent of this pest in Michiga...

  1. Rozšíření rýhovců Rhysodes sulcatus (Fabricius, 1787) a Omoglymmius germari (Ganglbauer, 1892) (Coleoptera: Rhysodidae) v České republice

    Czech Academy of Sciences Publication Activity Database

    Konvička, Ondřej; Čížek, Lukáš

    2015-01-01

    Roč. 6, Sept 1 (2015), s. 111-114 ISSN 1804-2732 R&D Projects: GA ČR GAP504/12/1952 Institutional support: RVO:60077344 Keywords : Coleoptera * distributional maps * faunistics Subject RIV: EH - Ecology, Behaviour

  2. Los ejemplares tipo de Dryopidae, Elmidae, Limnichidae y Psephenidae (Coleoptera: Byrrhoidea, depositados en la colección del Museo de La Plata The types of Dryopidae, Elmidae, Limnichidae and Psephenidae (Coleoptera: Byrrhoidea housed at the Museo de La Plata

    Directory of Open Access Journals (Sweden)

    Liliana A. Fernández

    2004-07-01

    Full Text Available Se examinaron los ejemplares tipo depositados en la colección de la División Entomología del Museo de La Plata, pertenecientes a diversas especies nominales de Byrrhoidea: Dryopidae (1 especie, 1 ejemplar tipo, Elmidae (4, 9, Limnichidae (29, 47 y Psephenidae (1, 2. El material tipo fue estudiado por los siguientes autores: Pic, Spangler, Spangler & Brown, Spangler & Perkins, Spangler & Santiago, Spilman y Wooldridge. Para cada taxón se indica la publicación original, datos de colección y condición del material.The types of species of Byrrhoidea (Coleoptera housed in the collection of the División Entomología of Museo de La Plata were examined and listed. They belong to 35 species distributed in the families Dryopidae (1 species, 1 type, Elmidae (4, 9, Limnichidae (29, 47 and Psephenidae (1, 2. The types were described by Pic, Spangler, Spangler & Brown, Spangler & Perkins, Spangler & Santiago, Spilman, and Wooldridge. For each taxon, complete information about categories of types, reference original descriptions, collection data and specimen condition are given.

  3. Description of the male of Eburella pinima Martins and notes on the geographical distribution of Eburodacrys aenigma Galileo & Martins and Eburodacrys lanei Zajciw (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Juan Pablo Botero

    2013-12-01

    Full Text Available Description of the male of Eburella pinima Martins and notes on the geographical distribution of Eburodacrys aenigma Galileo & Martins and Eburodacrys lanei Zajciw (Coleoptera, Cerambycidae. The male of Eburella pinima Martins, 1997 is described and illustrated for the first time. Information on Eburodacrys aenigma Galileo & Martins, 2006, previously known only from the female holotype, which lacked locality label, is herein complemented. This species is recorded from Brazil and the male is depicted for the first time. The geographical distribution of Eburodacrys lanei Zajciw, 1958 is further restricted here as some previous records are confirmed to result from misidentifications of E. aenigma.

  4. Bioatividade da azadiractina e de inseticidas sintéticos sobre a broca-do-café, Hypothenemus hampei (FERRARI, 1867) (COLEOPTERA: CURCULIONIDAE), em laboratório

    OpenAIRE

    Tatiane Lobak

    2016-01-01

    A broca-do-café, Hypothenemus hampei (Ferrari, 1867) (Coleoptera: Curculionidae), é praga limitante à produtividade e a qualidade dos grãos. Com a proibição do uso do endossulfam, para seu controle, em 2013, novas alternativas devem ser pesquisadas, visto que atualmente tem se seis princípios ativos registrados no Brasil, incluindo a azadiractina. Assim, o objetivo deste trabalho foi avaliar a bioatividade da azadiractina e de inseticidas sintéticos sobre a broca-do-café, em condições de labo...

  5. A new species of endogean, anophthalmous Parazuphium Jeannel from Northern Morocco (Coleoptera: Carabidae, with new molecular data for the tribe Zuphiini

    Directory of Open Access Journals (Sweden)

    Carmelo Andújar

    2011-06-01

    Full Text Available A new species of the genus Parazuphium (Coleoptera, Carabidae, Zuphiini, Parazuphium aguilerai sp.n., is described from the Tingitan peninsula in North Morocco. The single known specimen was found below a large stone deeply inserted in the substratum, and it is anophthalmous, depigmented and flattened. This is the second species of blind Parazuphium, the other being P. feloi Machado 1998 from a lave tube in the Canary Islands. Molecular data of the unique known P. aguilerai sp.n. specimen is provided, and a molecular phylogeny confirm its inclusion inside Zuphiini within Harpalinae. Identification keys to the Mediterranean and Macaronesian species of Parazuphium are provided.

  6. The infestation by an exotic ambrosia beetle, Euplatypus parallelus (F. (Coleoptera: Curculionidae: Platypodinae of Angsana trees (Pterocarpus indicus Willd. in southern Thailand

    Directory of Open Access Journals (Sweden)

    Sara Bumrungsri

    2008-07-01

    Full Text Available An exotic ambrosia beetle, Euplatypus parallelus (F. was collected from infested Pterocarpus indicus Willd. trees in Prince of Songkla University. Larvae and eggs were found in simple galleries with a single branch. Either a single male or a male and a female were found in each gallery. Half of these infested trees were previously attacked by long-horned beetles probably Aristobia horridula (Hope (Coleoptera: Cerambycidae, while some of them appeared to be healthy. Fusarium oxysporum Schlecht.:Fr. was isolated from frass, sapwood samples and insect larvae, and might be a cause of death of P.indicus.

  7. Caracterização Morfológica de Três Espécies de Broca-da-Haste em Chuchuzeiro (Coleoptera: Cerambycidae, Lamiinae)

    OpenAIRE

    SOUZA FILHO, MIGUEL F. DE; GABRIEL, DALVA; AZEVEDO FILHO, JOAQUIM A. DE

    2001-01-01

    A ocorrência simultânea de Adetus analis (Haldeman), Adetus fuscoapicalis Breuning e Plerodia syrinx (Bates) (Coleoptera: Cerambycidae, Lamiinae), broqueando ramos de chuchuzeiro Sechium edule (Jaqc.) Sw., é relatada. A. analis é sinônima de A. muticus (Thomson), que foi referida por diversos autores no Brasil. A. analis e A. fuscoapicalis são muito semelhantes quanto aos aspectos morfológicos e etológicos. Assim foi realizada uma descrição detalhada e ilustrações de ambas espécies a fim de p...

  8. Design and deployment of semiochemical traps for capturing Anthonomus rubi Herbst (Coleoptera: Curculionidae) and Lygus rugulipennis Poppius (Hetereoptera: Miridae) in soft fruit crops

    DEFF Research Database (Denmark)

    Fountain, Michelle T.; Baroffio, Catherine; Borg-Karlson, Anna-Karin

    2017-01-01

    Strawberry blossom weevil (SBW), Anthonomus rubi Herbst (Coleoptera: Curculionidae) and European tarnished plant bug (ETB), Lygus rugulipennis Poppius (Hetereoptera: Miridae), cause significant damage to strawberry and raspberry crops. Using the SBW aggregation pheromone and ETB sex pheromone we...... if deployed at ground level and although a cross vane was not important for catches of ETB it was needed for significant captures of SBW. The potential for mass trapping SBW and ETB simultaneously in soft fruit crops is discussed including potential improvements to make this more effective and economic...

  9. Biological activities of Zingiber officinale (Zingiberaceae) and Piper cubeba (Piperaceae) essential oils against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae).

    Science.gov (United States)

    Chaubey, Mukesh Kumar

    2013-06-01

    Zingiber officinale (Zingiberaceae) and Piper cubeba (Piperaceae) was essential oils were investigated for repellent, insecticidal, antiovipositional, egg hatching, persistence of its insecticidal activities against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae). Essential oil vapours repelled bruchid adults significantly as oviposition was found reduced in choice oviposition assay. Z. officinale and P. cubeba essential oils caused both fumigant and contact toxicity in C. chinensis adults. In fumigation toxicity assay, median lethal concentrations (LC50) were 0.34 and 0.27 microL cm(-3) for Z. officinale and P. cubeba essential oils, respectively, while in contact toxicity assay, LC50 were 0.90 and 0.66 microL cm(-2) for Z. officinale and P. cubeba essential oils, respectively. These two essential oils reduced oviposition in C. chinensis adults when treated with sublethal concentrations by fumigation and contact method. Oviposition inhibition was more pronounced when adults come in contact than in vapours. Both essential oils significantly reduced egg hatching rate when fumigated. Persistence in insecticidal efficiency of both essential oils decreased with time. P. cubeba showed less persistence than Z. officinale essential oil because no mortality was observed in C. chinensis adults after 36 h of treatment with P. cubeba and after 48 h of treatment of Z. officinale essential oil. Fumigation with these essential oils has no effect on the germination of the cowpea seeds. Findings of the study suggest that Z. officinale and P. cubeba essential oils can be useful as promising agent in insect pest management programme.

  10. Consumption of Bt Maize Pollen Containing Cry1Ie Does Not Negatively Affect Propylea japonica (Thunberg (Coleoptera: Coccinellidae

    Directory of Open Access Journals (Sweden)

    Yonghui Li

    2017-03-01

    Full Text Available Propylea japonica (Thunberg (Coleoptera: Coccinellidae are prevalent predators and pollen feeders in East Asian maize fields. They are therefore indirectly (via prey and directly (via pollen exposed to Cry proteins within Bt-transgenic maize fields. The effects of Cry1Ie-producing transgenic maize pollen on the fitness of P. japonica was assessed using two dietary-exposure experiments in the laboratory. In the first experiment, survival, larval developmental time, adult fresh weight, and fecundity did not differ between ladybirds consuming Bt or non-Bt maize pollen. In the second experiment, none of the tested lethal and sublethal parameters of P. japonica were negatively affected when fed a rapeseed pollen-based diet containing Cry1Ie protein at 200 μg/g dry weight of diet. In contrast, the larval developmental time, adult fresh weight, and fecundity of P. japonica were significantly adversely affected when fed diet containing the positive control compound E-64. In both experiments, the bioactivity of the Cry1Ie protein in the food sources was confirmed by bioassays with a Cry1Ie-sensitive lepidopteran species. These results indicated that P. japonica are not affected by the consumption of Cry1Ie-expressing maize pollen and are not sensitive to the Cry1Ie protein, suggesting that the growing of Bt maize expressing Cry1Ie protein will pose a negligible risk to P. japonica.

  11. Dietary fatty acids influence the growth and fatty acid composition of the yellow mealworm Tenebrio molitor (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Dreassi, Elena; Cito, Annarita; Zanfini, Assunta; Materozzi, Lara; Botta, Maurizio; Francardi, Valeria

    2017-03-01

    Fat is the second most abundant component of the nutrient composition of the mealworm Tenebrio molitor (Coleoptera: Tenebrionidae) that represents also an interesting source of PUFA, especially n-6 and n-3 fatty acids, involved in prevention of cardiovascular diseases. This study investigated the possibility of modifying the fat content and the FA composition of yellow mealworms through feeding and how this would be influenced by developmental stages, pupal sex, and generation with the future aim of applying this coleopteran as a diet supplement for human health. Growth rate and cumulative mortality percentage on the different feeding substrates were also evaluated to select the optimal conditions for a mass-raising of this insect species. Despite the different fat content in the six different breeding substrates used, T. molitor larvae and pupae contained a constant fat percentage (>34% in larvae and >30% in pupae). A similar total fat content was found comparing larvae and male and female pupae of the second generation to those of the first generation. On the contrary, FA composition differed both in larvae and pupae reared on the different feeding substrates. However, the exemplars reared on the diets based on 100% bread and 100% oat flour showed SFA, PUFA percentages, and an n-6/n-3 ratio more suitable for human consumption; the diet based on beer yeast, wheat flour, and oat flour resulted in a contemporary diet that most satisfied the balance between a fat composition of high quality and favorable growth conditions.

  12. The influence of synthetic food additives and surfactants on the body weight of larvae of Tenebrio molitor (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    V. O. Martynov

    2017-08-01

    Full Text Available The broad spectrum of negative effects of food additives and surfactants on living organisms and the environment in general indicate a necessity of a detailed study on this issue. The aim of this article is to evaluate the impact of food additives and surfactants in a concentration of 350 mg/kg of fodder on the body weight of third age Tenebrio molitor Linnaeus, 1758 (Coleoptera, Tenebrionidae larvae. A significant change in the body weight of T. molitor larvae was observed when they consumed a diet containing 350 mg/kg of sodium glutamate, sodium cyclamate and sodium benzoate. We observed a tendency towards increase in body weight after addition of the food colouring Allura Red, saccharin, benzoic acid, betaine, emulsifying wax, AOS and SLES, and also we observed a decrease in body weight after addition of Tartrazine and Indigo Carmine in the same concentration. Out of the 18 tested food additives, 3 significantly stimulated an increase in the body weight of third age T. molitor larvae, and 3 manifested the same effect at the level of tendency (stimulated an increase in mass on average by 43–58% over the 14-day experiment, and 2 caused decrease in the body weight of larvae. Also, the 4 studied surfactants manifested a tendency towards increase in the body weight of T. molitor. This study on the impact of food additives and surfactants on organisms of insects is of great significance for protecting rare species of insects.

  13. Aerial release of Rhinoncomimus latipes (Coleoptera: Curculionidae) to control Persicaria perfoliata (Polygonaceae) using an unmanned aerial system.

    Science.gov (United States)

    Park, Yong-Lak; Gururajan, Srikanth; Thistle, Harold; Chandran, Rakesh; Reardon, Richard

    2018-01-01

    Rhinoncomimus latipes (Coleoptera: Curculionidae) is a major biological control agent against the invasive plant Persicaria perfoliata. Release of R. latipes is challenging with the current visit-and-hand release approach because P. perfoliata shows a high degree of patchiness in the landscape, possesses recurved barbs on its stems, and often spreads into hard-to-access areas. This 3-year study developed and evaluated unmanned aerial systems (UAS) for precise aerial release of R. latipes to control P. perfoliata. We have developed two UAS (i.e. quad-rotor and tri-rotor) and an aerial release system to disseminate R. latipes. These include pods containing R. latipes and a dispenser to accommodate eight pods. Results of field tests to evaluate the systems showed no significant (P > 0.05) effects on survivorship and feeding ability of R. latipes after aerial release. Our study demonstrates the potential of UAS for precision aerial release of biological control agents to control invasive plants. The aerial deployment systems we have developed, including both pods and a dispenser, are low cost, logistically practical, and effective with no negative effects on aerially released R. latipes. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  14. The Capturing of the Apple Blossom Beetle, Tropinota hirta (Poda (Coleoptera: Scarabaeidae, by Different Traps in Afyonkarahisar

    Directory of Open Access Journals (Sweden)

    Bülent YAŞAR

    2014-03-01

    Full Text Available This study was conducted to determine the most effective traps for capturing Tropinota hirta (Poda (Coleoptera: Scarabaeidae being an economical pest on the flowers of apple trees in Sultandağı county of Afyonkarahisar province in 2010. Blue plastic traps of in the same color (Sticky plate trap, funnel plus water and large bowl plus water and also an attractant containing cinnamyl alcohol and trans-anethol, as a commercial preparation were used in the present study. Four different selected sites were investigated to find the most attractive traps for the mentioned pest. The highest number of captives was achieved in the first (43.60% of total and third (33.13% of total location sites. They were found in the locations second and the fourth as 14.53% and 8.72%, respectively. Most of the adults were captured by the blue funnel plus water traps with the attractants. We assume that the number of apple blossom beetles captured in apple orchards depends upon the age of the trees and whether they are near uncultivated or grain growing areas. In conclusion, we propose that the use of a blue funnel in a cup containing water, and also with an attractant, is an effective biotechnical method for controll ing pest existing on apple trees when chemicals cannot be applied during the blooming period.

  15. Updated distribution of Osmoderma eremita in Abruzzo (Italy and agro-pastoral practices affecting its conservation (Coleoptera: Scarabaeidae

    Directory of Open Access Journals (Sweden)

    Patrizia Giangregorio

    2015-12-01

    Full Text Available New records of Osmoderma eremita (Scopoli, 1763 (Coleoptera: Scarabaeidae: Cetoniinae are reported for Abruzzo (Italy, together with a review of its distribution in this region. O. eremita is a saproxylic beetle dependent on the presence of hollow deciduous trees with abundant wood mould in their cavities. The major threats for the species are habitat loss and fragmentation. EU Habitats Directive requests to the member States its protection and the monitoring of its conservation status. Detection of its occurrence is the first step to protect the species. The surveys have been carried out in ten sites of Abruzzo by using black cross-windows traps baited with specific pheromone. The species has been recorded for the first time in the Sant’Antonio forest and its presence is confirmed in the Peligna Valley, after a decade. The populations seem to be confined to small patches of suitable habitats. At local level, the abandonment of the pollarding practice (willow and beech forests and the use of pollarded trees as biomass for fuel are the major threats for this species. Indeed some key actions, such as the protection of old hollow trees and the continuation of pollarding practice in rural landscape, could be key factors for the conservation strategies of the species in the study area.

  16. Receptor chirality and behavioral specificity of the boll weevil,Anthonomus grandis Boh. (Coleoptera: Curculionidae), for its pheromone, (+)-grandisol.

    Science.gov (United States)

    Dickens, J C; Mori, K

    1989-02-01

    Electrophysiological recordings from antennal olfactory receptors and field behavioral experiments showed both male and female boll weevils,Anthonomus grandis Boh. (Coleoptera: Curculionidae), to respond specifically to (+)-grandisol, an enantiomer of compound I of the boll weevil aggregation pheromone. Single-cell recordings revealed antennal olfactory neurons in both male and female weevils keyed to (+)-grandisol. Electroantennograms in response to serial dilutions of the grandisol enaniiomers showed a threshold 100 to 1000 times lower for (+)-grandisol relative to its antipode. In field behavioral experiments, both sexes were significantly more attracted to (+)-grandisol in combination with the three other pheromone components than the combination with (-)-grandisol. When (-)-grandisol was placed with the (+)-enantiomer at equal dosages, a slight although statistically insignificant inhibition occurred. Subsequent field tests showed that the low level of attraction exhibited by (-)-grandisol in combination with the other three pheromone components could be attributed to the other three components alone. These results are in contrast with an earlier study, which found (-)-grandisol to be as attractive as the (+)-enantiomer.

  17. Effects of irradiated diet on longevity and prolificity of successive generations of Zabrotes subfasciatus (Boheman, 1833) (Coleoptera, Bruchidae)

    International Nuclear Information System (INIS)

    Domarco, R.E.

    1981-01-01

    The study was done in the laboratory of Entomology of the Centro de Energia Nuclear na Agricultura (CENA), University of Sao Paulo, in Piracicaba, State of Sao Paulo, Brazil. This research was carried out to observe the effect of irradiated beans (Phaseolus vulgaris, (L.), cultivar Rosinha) on the longevity and prolificity of Zabrotes subfasciatus (Boheman, 1833) (Coleoptera, Bruchidae). The first part of the study included the rearing of five generations of Zabrotes subfasciatus which were fed with irradiated beans (0, 10, 20, 100 e 200 krad, with a dose rate of approximately 70 krad/h). The second one consisted of growth of seven generations on irradiated beans (0, 100, 500, 1500 and 3000 krad, with a dose rate of approximately 70 krad/h). The data were obtained through daily countings of insect birth and death rates. It was concluded that irradiation modified the nutritional quality of beans, affecting the development of Zabrotes subfasciatus. This effect was studied through the longevity and prolificity, which were altered as compared with the control, once they were higher for the dosis of 1500 and 3000 krad. (author)

  18. Control of Eucryptorrhynchus scrobiculatus (Coleoptera: Cuculionidae), a Major Pest of Ailanthus altissima (Sapindales: Simaroubaceae), Using a Modified Square Trap Net.

    Science.gov (United States)

    Yang, Kailang; Wen, Xiaojian; Ren, Yuan; Wen, Junbao

    2018-04-19

    Eucryptorrhynchus scrobiculatus (Motschulsky) (Coleoptera: Cuculionidae) is a borer that mainly attacks the tree of heaven, Ailanthus altissima (Mill.) Swingle (Sapindales: Simaroubaceae), and is one of the most damaging forestry pests in China. We developed a trap net for entangling and immobilizing soil-emerging weevils in order to reduce their impact. Recapture rates of weevils in the laboratory was significantly higher with nylon netting of 9, 10, or 11 mm mesh sizes than larger sizes, and these sizes were used to make trial nets for preventing weevil emergence from the soil around impacted trees in the field. Nets were 2 × 2 m with a reinforced border and Velcro-closable, radial slit which allowed the net to be arranged around the base of the tree while producing an unbroken barrier beneath the soil surface (i.e., a modified square trap net, MSTN). Recapture rates of weevils released in the soil did not differ among the MSTNs of 9, 10, or 11 mm mesh sizes. MSTN treatments significantly reduced emergence by naturally-occurring weevils from the soil surrounding trees and reduced numbers of weevils caught in population monitoring traps deployed in treated stands. The results demonstrated that MSTNs might be used to manage of E. scrobiculatus.

  19. Economic injury level for the coffee berry borer (Coleoptera: Curculionidae: Scolytinae) using attractive traps in Brazilian coffee fields.

    Science.gov (United States)

    Fernandes, F L; Picanço, M C; Campos, S O; Bastos, C S; Chediak, M; Guedes, R N C; Silva, R S

    2011-12-01

    The currently existing sample procedures available for decision-making regarding the control of the coffee berry borer Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) are time-consuming, expensive, and difficult to perform, compromising their adoption. In addition, the damage functions incorporated in such decision levels only consider the quantitative losses, while dismissing the qualitative losses. Traps containing ethanol, methanol, and benzaldehyde may allow cheap and easy decision-making. Our objective was to determine the economic injury level (EIL) for the adults of the coffee berry borer by using attractant-baited traps. We considered both qualitative and quantitative losses caused by the coffee borer in estimating the EILs. These EILs were determined for conventional and organic coffee under high and average plant yield. When the quantitative losses caused by H. hampei were considered alone, the EILs ranged from 7.9 to 23.7% of bored berries for high and average-yield conventional crops, respectively. For high and average-yield organic coffee the ELs varied from 24.4 to 47.6% of bored berries, respectively. When qualitative and quantitative losses caused by the pest were considered together, the EIL was 4.3% of bored berries for both conventional and organic coffee. The EILs for H. hampei associated to the coffee plants in the flowering, pinhead fruit, and ripening fruit stages were 426, 85, and 28 adults per attractive trap, respectively.

  20. Attraction of Tomicus yunnanensis (Coleoptera: Scolytidae to Yunnan Pine Logs with and without Periderm or Phloem: An Effective Monitoring Bait

    Directory of Open Access Journals (Sweden)

    Rong Chun Lu

    2012-01-01

    Full Text Available The Yunnan pine shoot beetle, Tomicus yunnanensis Kirkendall and Faccoli (Coleoptera: Scolytinae is an important pest of Yunnan pine (Pinus yunnanensis Franch in China. Experiments with host log baits were done to develop a pest monitoring system using host tree kairomone. Five Yunnan pine logs (each 10–15 cm diam. × 30-cm long in a trap-log bundle were treated by peeling periderm (outer bark off to expose the phloem, and half of each log was covered with sticky adhesive to capture any attracted adult beetles. Significantly, more beetles were attracted and caught on the periderm-peeled logs (ca 30 beetles/m2 log surface/day than on untreated control logs with adhesive (ca 2.5/m2/day. No significant differences were observed between catches on logs taken from lower or upper halves of Yunnan pines. T. yunnanensis flies mostly during the afternoon according to trap catches throughout the day. Attraction to the periderm-peeled logs decreased considerably when they were peeled further to remove the phloem, indicating phloem volatiles play a role in selection of the host by the beetle. The readily-available log baits appear useful for monitoring pine shoot beetle populations in integrated pest management programs.