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Sample records for apagomerella versicolor coleoptera

  1. Tinea versicolor

    Science.gov (United States)

    ... JavaScript. Tinea versicolor is a long-term (chronic) fungal infection of the skin . Causes Tinea versicolor is fairly ... back References Hay RJ. Dermatophytosis and other superficial mycoses. In: Mandell GL, Bennett JE, Dolin R, eds. ...

  2. Tinea Versicolor

    Science.gov (United States)

    ... resources Meet our partners Español Donate Diseases and treatments Acne and rosacea Bumps and growths Color problems Contagious skin diseases ... Tinea versicolor public SPOT Skin Cancer™ Diseases and treatments Acne and rosacea Bumps and growths Color problems Acanthosis nigricans Melasma ...

  3. Pityriasis versicolor alba.

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    Thoma, W; Krämer, H-J; Mayser, P

    2005-03-01

    Pityriasis versicolor alba is a hypopigmented or depigmented variant of pityriasis versicolor characterized by maculous, partly pityriasiform, scaly depigmented lesions occurring particularly in seborrhoeic areas. Long-persisting hypopigmentation after healing of the pityriasis versicolor was first described by Gudden in 1853. Hypopigmentation and depigmentation were later differentiated as an independent variant of the disease. In 1848, Eichstedt recognized the pathogen-related character of pityriasis versicolor in its hyperpigmented form. Today it is generally accepted that the disease is caused by yeasts of the genus Malassezia, of which nine species are differentiated. It is controversial whether a single species is responsible for the disease. The pathogenesis of depigmentation has not been established. A screening effect by the scale layer as well as toxic effects on pigment synthesis by fungal metabolites have been discussed. With regard to the second mechanism, the newly discovered tryptophan-derived metabolites of M. furfur might be significant. Evidence-based data concerning the therapy of pityriasis versicolor alba do not exist. According to current recommendations, pityriasis versicolor should be rapidly treated with antimycotics, followed by ultraviolet therapy to induce maturation of existent melanosomes and accelerate repigmentation. However, depigmented lesions are difficult to improve by ultraviolet therapy.

  4. Pitiríase Versicolor Pityriasis Versicolor

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    Josenildo Rodrigues de Oliveira

    2002-10-01

    Full Text Available A expressão pitiríase versicolor define uma infecção fúngica superficial caracterizada por alterações na pigmentação cutânea. O distúrbio de pigmentação é devido à colonização do estrato córneo por um fungo dimórfico, lipofílico, encontrado na flora normal da pele, conhecido como Malassezia furfur. Trata-se de doença prevalente nos trópicos, mas também comum em climas temperados. Há vários tratamentos disponíveis com taxas elevadas de cura, porém as recorrências são freqüentes.Pityriasis versicolor is a superficial fungal infection characterized by changes in skin pigment due to colonization of the stratum corneum by a dimorphic lipophilic fungus of the normal flora of the skin, known as Malassezia furfur. This disease is most prevalent in the tropics, but is also common in temperate climates. Treatment is available and cure rates are high, although recurrence is common.

  5. Uncommon cases of pityriasis versicolor.

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    Romano, C

    2008-06-01

    Malassezia may play a role in several dermatoses. It is responsible for foliculitis and mainly for pityriasis versicolor. Pityriasis versicolor is the most known dermatitis because of its clinical aspects and frequently for its poor response to the therapy, mainly in chronic forms. The clinical aspects of uncommon and rare forms of pityriasis versicolor have been reported. The data related to the patients observed in the last thirty years in Siena are reported. In addition, a study was carried out in Pisa by Professor F. Mancianti to identify species of Malassezia isolated in 37 patients.

  6. Blaschkoid pityriasis versicolor.

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    Tan, Cheng; Zhu, Wen-Yuan; Min, Zhong-Sheng

    2010-07-01

    A 31-year-old male patient complained of having follicular and brownish red maculopapules along the Blaschko's lines on the right chest for 2 days. On examination, follicular brownish maculopapules were present on the chest with a uniform size of about 3 mm in diameter. The lesions were isolated without a tendency to merge, giving several S-shaped, band-like appearances. Direct mycological examination of the skin flakes revealed many pseudomycelial hyphae and yeast cells with typical spaghetti and meatball appearance. Wood's light examination of the lesion revealed a golden yellow fluorescence. A diagnosis of blaschkoid pityriasis versicolor was suggested because of blaschkoid distribution of the lesions in this new variant of PV.

  7. Oral fluconazole in tinea versicolor

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    Sankara Rao I

    1997-01-01

    Full Text Available 25 patients with extensive tinea versicolor were treated with single oral dose of 400 mg of fluconazole. 25 patients returned for follow-up. Follow-up at 3 weeks, 6 weeks and 8 weeks showed 100% clinical cure rate and 92% mycological cure rate. No significant side effects were noticed. The majority of patients found the treatment effective, safe and convenient.

  8. Triterpenoid saponins from Dianthus versicolor.

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    Ma, Li; Gu, Yu-Cheng; Luo, Jian-Guang; Wang, Jun-Song; Huang, Xue-Feng; Kong, Ling-Yi

    2009-04-01

    Seven new pentacyclic triterpenoid saponins, named dianversicosides A-G (1-7), together with nine known compounds, were isolated from the aerial parts of Dianthus versicolor. The structures of 1-7 were elucidated on the basis of spectroscopic data and chemical evidence. The absolute configuration of the 3-hydroxyl-3-methylglutaryl (HMG) group in 1-4 was ascertained by chemical analysis combined with a chiral HPLC method. Cytotoxic activities of the isolated compounds were evaluated against a small panel of cancer and other cell lines.

  9. Bilateral areolar and periareolar pityriasis versicolor.

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    Sárdy, Miklós; Korting, Hans Christian; Ruzicka, Thomas; Wolff, Hans

    2010-08-01

    An adolescent boy presented with isolated, symmetrical, bilateral areolar and periareolar pityriasis versicolor. This extremely rare condition should be considered in the differential diagnosis of light brown patches on the areolae.

  10. Tinea versicolor in dark-skinned individuals.

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    Kallini, Joseph R; Riaz, Fauzia; Khachemoune, Amor

    2014-02-01

    In this article, we review the salient features of tinea versicolor and describe the epidemiology, clinical presentation, and histopathology of this mycosis in dark-skinned individuals. Tinea versicolor is caused by an overgrowth of the Malassezia genus. It manifests clinically as asymptomatic hypopigmented macules, hyperpigmented macules, or a combination of the two. Under light microscopy, Malassezia presents as a dimorphic fungus - in both the hyphal and yeast form. Most clinicians have found that the majority of dark-skinned patients present solely with hypopigmented lesions. Under light microscopy, lesions on dark skin involved with tinea versicolor tend to have a thicker stratum corneum, more tonofilaments in the granulosum, and more sequestered melanosomes. Differential diagnosis includes confluent and reticulated papillomatosis, seborrheic dermatitis, pityriasis rosea, pityriasis alba, and vitiligo. Tinea versicolor can be successfully managed in most cases with topical antifungal treatments. Cases of recurrence, such as those seen in immunocompromised patients, may necessitate scheduled oral or topical therapy. PMID:24320140

  11. Malassezia Species and Pityriasis Versicolor

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    Gulin Rodoplu

    2016-01-01

    Full Text Available Malassezia species are found in part of the normal human cutaneous commensal flora, however it has been known for many years that the Malassezia yeasts are associated with a number of different human diseases ranging from pityriasis versicolor to seborrhoeic dermatitis. In addition, since the 1980s, they have been reported as causing opportunistic systemic infections. The taxonomy of Malassezia spp. has recently been modified to include 13 obligatorily lipophilic species, plus one non-obligatorily lipophilic species, which only rarely colonizes human hosts and currently the genus consist 14 species as M. furfur, M. pachydermatis, M. sympodialis, M. globosa, M. obtusa, M. slooffiae, M. restricta, M. dermatis, M. japonica, M. nana, M. yamatoensis, M. caprae, M. equina, M. cuniculi. Fastidious growth requirements of Malassezia yeasts defied the initial attempts to culture these organisms and their true identification and the relationship between different species only became apparent with the application of modern molecular techniques. The causative fungus is seen especially in such seborrheic areas as the scalp, face, trunk and upper back. Under the influence of various exogenous or endogenous predisposing factors, these yeasts change from the blastospore form to the mycelial form and become pathogenic. Diagnosis of pityriasis versicolor which is caused by Malassezia species is generally easy and lies on the basis of its clinical appearance and can be confirmed by mycological examination. The diagnosisis is mainly based on direct examination with potassium hydroxide (KOH and demonstration that represents pseudohyphae and blastoconidia as the typical %u201Cspaghetti and meatballs%u201D pattern. Characteristic features of the genus Malassezia include a distinctive morphology and an affinity for lipids in culture. Culture is necessary to recover the infecting strain, especially for epidemiologic purposes and also to test its antifungal susceptibility

  12. Management of seborrheic dermatitis and pityriasis versicolor.

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    Faergemann, J

    2000-01-01

    Pityriasis (tinea) versicolor and seborrheic dermatitis are two very common skin diseases. Pityriasis versicolor is a chronic superficial fungal disease usually located on the upper trunk, neck, or upper arms. In pityriasis versicolor, the lipophilic yeast Malassezia (also know as Pityrosporum ovale or P. orbiculare) changes from the blastospore form to the mycelial form under the influence of predisposing factors. The most important exogenous factors are high temperatures and a high relative humidity which probably explain why pityriasis versicolor is more common in the tropics. The most important endogenous factors are greasy skin, hyperhidrosis, hereditary factors, corticosteroid treatment and immunodeficiency. There are many ways of treating pityriasis versicolor topically. Options include propylene glycol, ketoconazole shampoo, zinc pyrithione shampoo, ciclopiroxamine, selenium sulfide, and topical antifungals. In difficult cases, short term treatment with fluconazole or itraconazole is effective and well tolerated. To avoid recurrence a prophylactic treatment regimen is mandatory. Seborrheic dermatitis is characterized by red scaly lesions predominantly located on the scalp, face and upper trunk. There are now many studies indicating that Malassezia plays an important role in this condition. Even a normal number of Malassezia will start an inflammatory reaction. Mild corticosteroids are effective in the treatment of seborrheic dermatitis. However, the disease recurs quickly, often within just a few days. Antifungal therapy is effective in the treatment of seborrheic dermatitis and, because it reduces the number of Malassezia, the time to recurrence is increased compared with treatment with corticosteroids. Antifungal therapy should be the primary treatment of this disease.

  13. Atypical pityriasis versicolor case report

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    Zonunsanga

    2015-04-01

    Full Text Available Pityriasis versicolor is a superficial fungal infection caused by mycelial form of Malassezia spp, which is confined to stratum corneum. It usually present in the trunk as either hypo or hyperpigmented, aymptomatic, round to oval macules of varying sizes, which may merged to form geographic shape. Diagnosis is usually done clinically, or KOH examination which shows typical spagetti and meat balls appearances, or even by wood’s lamp which shows orange to yellow fluorescence. The case series had been recording in between 2012 to 2013. Within that period, we had recorded 32 cases. All the patients which we had recorded presented with multiple, asymptomatic macules of small sizes varying from 1-2 cm in diameter to 3-4mm in diameter, usually round to oval, hypopigmented, non scaly lesions. 26 patients had lesions on forearms, 3 patients had lesions on dorsa of hands bilaterally, 3 patients had similar kind of lesions on thigh. Besnier’s test was positive in 14 (43.75% patients. KOH examinations showed fungal hyphae in 14 (33.33% patients with typical spagetti and meat balls appearances in 9 (8.13% patients. All of them were given and all of them got response and healed within 2-4 months.

  14. Case report. Pityriasis versicolor mimicking Pityriasis rotunda.

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    Aste, Nicola; Pau, Monica; Aste, Natalia; Biggio, P

    2002-04-01

    Pityriasis versicolor is a common dermatomycosis, occurring throughout the world, characterized by irregular, slightly scaly patches, varying in color from red/light brown to white. Pityriasis rotunda, on the other hand, is an uncommon disease, reported in specific ethnic groups, and characterized by perfectly round or oval patches of varying color, with a scaly surface. The histologic pattern is that of ichthyosis vulgaris. We report here the case of a male patient, aged 31, from Sardinia (Italy), affected by Pityriasis versicolor mimicking Pityriasis rotunda. Mycological examination allowed us to formulate the correct diagnosis, and ensuing treatment with antifungal drugs was entirely successful. The authors, while pointing out the rarity of this case, stress the possibility that Pityriasis versicolor mimics Pityriasis rotunda and vice-versa, especially in those countries in which the two diseases are endemic. More widespread recourse to microscopic examination can help avoid the risk of mistaken diagnosis and consequent incorrect treatment.

  15. Fluconazole and itraconazole in pityriasis versicolor

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    Jaswal Ritu

    1999-01-01

    Full Text Available Pityriasis versicolor is a common superficial fungal infection caused by Malassezia species. It has a high incidence and prevalence in tropical climates. Although it responds well to treatment, relapses and recurrences are frequent. In the present study the therapeutic response of single dose fluconazole (400 mg with itraconazole (100mg twice daily ? 7 days was compared in sixty patients of pityriasis versicolor. No significant statistical difference (p>0.05% was observed between efficacy of two drugs. Therapy with fluconazole is preferable in view of single dose administration and lesser cost as compared to itraconazole.

  16. A Steroidal Glycoside from Cynanchum versicolor Bunge

    Institute of Scientific and Technical Information of China (English)

    Zhao Guang ZHENG; Run Hui LIU; Ling Yi KONG; Wei Dong ZHANG

    2006-01-01

    A new C21 steroidal glycoside, named cynanversicoside F (1), was isolated from the root of Cynanchum versicolor Bunge. Its structure was established as glaucogenin-A 3-O-β-D-digitoxopyranosyl-(1→4)-β-D-cymaropyranoside by spectroscopic and chemical methods.

  17. Hypopigmented macules: leprosy, atopy or pityriasis versicolor?

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    Massone, C; Cavalchini, A; Clapasson, A; Nunzi, E

    2010-12-01

    Lepromatous leprosy (LL) represents the highest infective and multibacillary form of leprosy. Clinical manifestations are consequent to the haematogenous spread of bacilli and include macules, plaques and nodules in a symmetric distribution or a diffuse infiltration of the skin. LL may mimic many different inflammatory and neoplastic skin diseases and in a small percentage of patients, skin manifestation may be atypical. This article reports the case of a South American child with LL presenting with symmetrically distributed hypopigmented macules previously misdiagnosed as pytiriasis alba, atopic dermatitis and pityriasis versicolor. Atopy and pityriasis versicolor are common skin conditions that can be also observed in leprosy patients and that can masquerade the diagnosis of LL, especially if occurring in dark skin. Dermatologists in Europe should be aware of this unusual form of presentation of leprosy and must take in mind Hansen disease in the differential diagnosis in patients coming from endemic areas.

  18. Atrophying pityriasis versicolor: is this a new variant of pityriasis versicolor?

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    Yang, Yun-Seok; Shin, Min-Kyung; Haw, Choong-Rim

    2010-11-01

    An atypical clinical form of pityriasis versicolor has been infrequently reported, in which cutaneous atrophy is associated with individual pityriasis versicolor lesions. The pathogenesis of this atrophy remains unclear, but is believed to be a delayed-type hypersensitivity reaction to antigens derived from the Malassezia species. A 60-year-old man presented with multiple, slightly scaly, and depressed maculopatches or plaques on the trunk and extremities. Our microscopic examination of the skin scrapings on a KOH preparation revealed numerous short hyphae and spores. The patient was treated daily with 200 mg of itraconazole in combination with topical antifungals, achieving clinical improvement and mycological recovery, which was confirmed upon follow-up 1 month later. This is the first case report of atrophying pityriasis versicolor in Korea. It needs to be differentiated from other atrophying disorders of the skin.

  19. CULTIVATION OF TRAMETES VERSICOLOR IN MEXICO

    OpenAIRE

    D. González Guerrero; V. Esparza Martínez; R. de la Torre Almaráz

    2011-01-01

    A native strain of Trametes versicolor (Coriolaceae) was isolated and cultivated under laboratory conditions. Mycelial colonies were off-white, showing high density, velvety texture, and abundant aerial hyphae. Substrates studied had good mycelial growth and colonization. Higher mushroom yield of 173.8 g was recorded on supplemented oak sawdust, reaching a biological efficiency of 20.3%. Leathery, dark brown fruit bodies were obtained having normal morphology. A lower biological efficiency of...

  20. Growth of Trametes versicolor on phenol.

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    Yemendzhiev, H; Gerginova, M; Krastanov, A; Stoilova, I; Alexieva, Z

    2008-11-01

    Trametes versicolor 1 was shown to grow on phenol as its sole carbon and energy source. The culture growth and degradation ability dependence on culture medium pH value was observed. The optimal pH value of a liquid Czapek salt medium was 6.5. The investigated strain utilized completely 0.5 g/l phenol in 6 days. The dynamics of the phenol degradation process was investigated. The process was characterized by specific growth rate micromax 0.33 h(-1), metabolic coefficient k=4.4, yield coefficient Yx/s=0.23 and rate of degradation Q=0.506 h(-1). The intracellular activities of phenol hydroxylase (0.333 U/mg protein) and cis,cis-muconate lactonizing enzyme (0.41 U/mg protein) were demonstrated for the first time in this fungus. In an attempt to estimate the occurrence of gene sequences in T. versicolor 1 related to phenol degradation pathway a dot blot analysis with total DNA isolated from this strain was performed. Two synthetic oligonucleotides were used as hybridizing probes. One of the probes was homologous to the 5'end of phyA gene coding for phenol hydroxylase in Trichosporon cutaneum ATCC 46490. The other probe was created on the basis of cis,cis-muconate lactonizing enzyme coding gene in T. cutaneum ATCC 58094. The results of these investigations showed that T. versicolor 1 may carry genes similar to those of Trichosporon cutaneum capable to degrade phenol.

  1. Ketoconazole 2% shampoo in pityriasis versicolor: An open trial

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    Rathi S

    2003-03-01

    Full Text Available Pityriasis versicolor is a common superficial fungal infection caused by a lipophilic yeast. The aim of the study was to evaluate the efficacy of ketoconazole 2% shampoo in the treatment of pityriasis versicolor, for which thirty patients were included. The shampoo was applied daily for 3 days and found to be very effective in clearing the signs and symptoms of the disease. There was no serious adverse effects.

  2. IN VITRO PROPAGATION OF ALTERNANTHERA VERSICOLOR (LINN.) - A MEDICINAL HERB

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    D. Preetha; K. Mahalakshmi; M. Bhakyalakshmi

    2013-01-01

    A complete protocol for micropropagation of Alternanthera versicolor using leaf and internodal explants was developed. An explants from A. versicolor established on Murashige and Skoog medium were treated with various concentrations and combinations of auxins and cytokinins to determine the best method for callus formation, shoot regeneration and root formation. MS medium supplemented with 1 mg/l BAP and 1 mg/l IAA was determined to be the most suitable for callus induction. After 2 weeks, th...

  3. Pityriasis versicolor in the pediatric age group

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    Jena Deepak

    2005-01-01

    Full Text Available BACKGROUND: Pityriasis versicolor (PV is a mild chronic infection of the skin caused by Malassezia yeasts. Although it is primarily seen in adults, children are often affected in the tropics . METHODS: Over a period of 2 years, children (up to the age of 14 years who were clinically and mycologically diagnosed as PV were included in the study. The clinical and epidemiological pattern in different age groups was noted. RESULTS: PV in this age group formed about 31% of the total cases of PV; 4.8% cases presented in infancy. The commonest site of involvement was the face in 39.9% of the cases. Most of the cases presented in summer months. CONCLUSIONS: PV is not an uncommon disease among children in the tropics. There is a sudden resurgence of cases in the hot monsoons and even infants are not spared.

  4. Fluconazole in the therapy of Pityriasis versicolor

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    Poljački Mirjana N.

    2005-01-01

    Full Text Available The authors present the results of the systemic application of flukonazole in therapy of Pityriasis versicolor. It was arranged for the total number of 38 patients, 18 females and 20 males. The diagnosis of diseases was established on the base of the clinical examination, the native mycological examination and by the using of Wood lamps. The therapy was passed by the using of 300 mg flukonazole in a single dose, once weekly, during two weeks. The following period amounted to one week after the passed therapy. The therapeutic efficacy was assessed with regard to the clinical and mycological healing. The clinical efficacy was assessed semiquantitative on the base of increasing of the percentage rates of the total score of disease that was computed by collecting of the numeric values for every clinical argument typical for the disease, and the mycological efficacy on the base of the mycological findings and the fluorescence finding after lightening with the Wood's lamp. The controlling examinations were performed on day 0, 14 and 22. The results of investigations have shown that the complete clinical healing was achieved after two weeks of therapy in 94,74%, and the mycological healing in 92,11% patients. The rate of the mycological healing was evaluated after 1 week of following period equal to the rate of the clinical healing and it was also 94,74%. The undesirable effects of the drug applications weren't by any patient.

  5. Acral pityriasis versicolor – A rare clinical presentation

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    Tasleem Arif

    2015-04-01

    Full Text Available Pityriasis versicolor is a superficial fungal infection of the skin caused by the yeast of the genus Malassezia and presents as hypo or hyper pigmented scaly macules. The most commonly affected sites include upper trunk, upper arms, neck and the abdomen. Lesions confined to the acral parts like hands and feet have rarely been reported. In this article the author reports a 40 year old male who presented with multiple hypo pigmented scaly macules confined to the acral parts (hands and wrist. The acral variant of pityriasis versicolor is considered to be a very rare clinical entity which prompted the author to report this case.

  6. Clinicomycological profile of pityriasis versicolor in Assam

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    Ajanta Sharma

    2016-01-01

    Full Text Available Background: Geographical variation in the distribution of Malassezia species associated with pityriasis versicolor (PV has led to the necessity of studying epidemiological, mycological, and clinical characteristics of PV. Aims: To study the epidemiological, mycological, and clinical characteristics of PV in a tertiary care hospital. Settings and Design: The study was carried out with a cross-sectional design. Materials and Methods: Two hundred and sixty-two consecutive PV patients were subjected to detailed history, clinical examination, and investigations. Skin scrapings were processed by direct microscopy and culture. Isolates were identified by phenotypic characteristics and polymerase chain reaction-restriction fragment length polymorphism. Association of Malassezia species with clinical and epidemiological characteristics was studied. Statistical analysis of the data was done using statistical software. Results: Maximum number of PV cases (33.9% belonged to the age group of 21–30 years with a male preponderance. 61.4% of the patients had a sedentary lifestyle, 70.2% showed the gradual onset of the disease, 51.1% presented with pruritus and in 66.4% of the patients symptoms were continuous. Most commonly involved body site was neck (27.8%, 77.09% of the lesions were bilaterally asymmetrical, 87.4% were macular, and 89.3% were hypopigmented. Malassezia furfur (77.3% was the predominant species. Sedentary lifestyle (61.4% and increased sweating (48% were the most commonly associated predisposing factors. Conclusion: PV is more common in males. Distribution of Malassezia species varies significantly from those reported in other parts of India. M. furfur was the most common species responsible for PV in our region. Hence, further studies are required to evaluate the exact cause of this variation.

  7. [Pityriasis versicolor in Greece and its predisposition factors].

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    Parisis, N; Stratigos, J; Marcelou-Kinti, U; Capetanakis, J

    1977-11-01

    The age and seasonal incidence of 2610 patients with pityriasis versicolor in Greece were studied. Determination of the pH of Na and K ions of the sweat and the microbiol flora of the skin of pityriasis versicolor patients was undertaken. Besides these the effectiveness of a 1% selenium disulfid suspension was tested. The results indicate that the age groups of 20-29 and 30-30 years are mostly affected by the disease. A high incidence of the skin manifestation was noted during the summer and fall months. A difference of the pH of the sweat between pityriasis versicolor and healthy controls was observed, but no difference was found in the Na and K ions of the sweat among these two groups. Neither did the microbial flora from the skin lesions of patients and from corresponding sites of controls show any difference. The high relapse in this experiment indicates the relative ineffectiveness of selenium disulfid preparations when used as a 1% suspension in the treatment of pityriasis versicolor.

  8. Influence of systemic corticotherapy on the triggering of pityriasis versicolor.

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    da Fraga, Clarissa Matarangas Moreira; de Cássia Birschiner, Rita; Naseri, Alice Pignaton; Diniz, Lucia Martins

    2014-09-01

    Pityriasis versicolor is a frequent mycosis and the use of systemic corticotherapy is one of its predisposing factors. This is an observational, cross-sectional, analytical and comparative study, conducted from January 2012 to January 2013 in the following outpatient clinics: Dermatology Service, Cassiano Antonio Moraes Hospital (HUCAM), Vitória, ES, Brazil; Nephrology Service, HUCAM; and Leprosy Department, Maruípe Health Unit, Vitória, ES, Brazil. Patients, undergoing long-term systemic corticotherapy (or not), were assessed with respect to the presence of pityriasis versicolor. If there was mycosis, a direct mycological examination would be carried out. The spss 17.0 software was used for the statistical analysis. From the total of 100 patients, nine had pityriasis versicolor, being eight from the corticotherapy group and one from the group with no use of corticosteroids. Regarding the patients with mycosis, the prevalent age ranged from 20 to 39 years, with six patients; six were women; seven mixed race; eight were undergoing long-term systemic corticotherapy; seven were taking low-dose systemic corticosteroids; four had leucocytosis; five had normal total cholesterol and triglycerides; and four had normal glycaemia. There was increased frequency of pityriasis versicolor in the group undergoing systemic corticotherapy with statistical significance, corroborating the only study on the topic (1962).

  9. Disruption of barrier function in dermatophytosis and pityriasis versicolor.

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    Lee, Weon Ju; Kim, Jun Young; Song, Chang Hyun; Jung, Hong Dae; Lee, Su Hyun; Lee, Seok-Jong; Kim, Do Won

    2011-11-01

    Dermatophytes have the ability to form molecular attachments to keratin and use it as a source of nutrients, colonizing keratinized tissues, including the stratum corneum of the skin. Malassezia species also affect the stratum corneum of the skin. Therefore, dermatophytosis and pityriasis versicolor of the skin are thought to be important factors of profound changes in skin barrier structure and function. We aimed to describe the changes in transepidermal water loss (TEWL), stratum corneum hydration, and skin pH in the lesions of the dermatophytosis and pityriasis versicolor. Thirty-six patients with dermatophytosis (14 with tinea cruris, 13 with tinea corporis and nine with tinea pedis or tinea manus) and 11 patients with pityriasis versicolor were included in this study. TEWL, stratum corneum conductance and skin pH were determined by biophysical methods to examine whether our patients exhibited changes in barrier function. Dermatophytosis and pityriasis versicolor except tinea pedis and tinea manus showed highly significant increase in TEWL compared with adjacent infection-free skin. Hydration was significantly reduced in lesional skin compared with adjacent infection-free skin. From this study, infections with dermatophytes and Malassezia species on the body can alter biophysical properties of the skin, especially the function of stratum corneum as a barrier to water loss. On the contrary, infections with dermatophytes on the palms and soles little affect the barrier function of the skin.

  10. Pityriasis versicolor: avoiding pitfalls in disease diagnosis and therapy.

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    Rivard, Shayna C

    2013-08-01

    Pityriasis versicolor is common among young active duty members with overactive sweat glands working in humid environments and results in pigmentary changes that can be profound in those with darker skin. This article addresses several issues related to making the correct diagnosis and providing appropriate treatment, as well as the specific challenges military providers may face in these cases.

  11. Trametes versicolor extract modifies human fecal microbiota composition in vitro.

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    Yu, Zhuo-Teng; Liu, Bo; Mukherjee, Purna; Newburg, David S

    2013-06-01

    Trametes versicolor is a mushroom used as a traditional Chinese medicine (Yun-zhi) for a wide array of seemingly disparate conditions. We hypothesized that many of its multiple purported activities could be mediated through stimulation of beneficial mutualist components of the microbiota. Human fecal microbiota was cultured anaerobically to determine its ability to ferment a common extract of T. versicolor, designated polysaccharide peptide (PSP), and the ability of PSP to alter the composition of the microbial community. The presence of PSP and fructooligosaccharides (FOS, a common prebiotic) in the medium, but not cellulose, significantly increased levels of Bifidobacterium spp. PSP also elevated Lactobacillus spp., while reducing Clostridium spp., Staphylococcus spp. and Enterococcus spp. Levels of Streptococcus spp., Bacteroides spp. and Escherichia did not significantly change. Fermentation of PSP increased the concentration of organic acids (lactate and short-chain fatty acids), decreased the pH, and induced β-galactosidase and β-glucosidase activities. The genera of the human microbiota that are promoted by FOS and other prebiotics are also stimulated by the Trametes versicolor extract, PSP. Thus, Trametes versicolor, a common East Asian botanical, contains putative prebiotic agents that alter human gut microbiota and pH. This prebiotic-like activity may help explain some of the plethora of the health benefits attributed to this traditional Chinese medicine. PMID:23435630

  12. The effect of surfactant on pollutant biosorption of Trametes versicolor

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    Gül, Ülküye Dudu; Silah, Hülya; Akbaş, Halide; Has, Merve

    2016-04-01

    The major problem concerning industrial wastewater is treatment of dye and heavy metal containing effluents. Industrial effluents are also contained surfactants that are used as levelling, dispersing and wetting agents. The purpose of this study was to investigate the effect of surfactant on textile dye biosorption properties of a white rot fungus named Trametes versicolor. Reactive dyes are commonly used in textile industry because of their advantages such as brightness and excellent color fastness. A recative textile dye, called Everzol Black, was used in this study. The low-cost mollasses medium is used for fungal growth. The usage of mollases, the sugar refinery effluent as a source of energy and nutrients, gained importance because of reducing the cost and also reusing another waste. In biosorption process the effect of surfactant on dye removal properties of T. versicolor was examined as a function of pH, dye consentration and surfactant concentration. The results of this study showed that the surfactant enhanced the dye removal capacity of Trametes versicolor. The dye and surfactant molecules were interacted electrostatically and these electrostatic interactions improved dye removal properties of filamentous fungus T. versicolor. The results of this study recommended the use of surfactants as an inducer in textile wastewater treatment technologies.

  13. Novos aspectos na evolução clínica da pitiríase versicolor New aspects in the clinical course of pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Valéria Maria de Souza Framil

    2011-12-01

    Full Text Available FUNDAMENTO: A pitiríase versicolor é uma doença infecciosa causada por várias espécies de Malassezia com uma tendência a se tornar recidivante ou crônica. OBJETIVOS: Este trabalho foi conduzido na tentativa de conhecer a evolução clínica da pitiríase versicolor em relação ao número de recidivas após um tratamento adequado no período de 12 meses e correlacionar o número de recidivas com as espécies de Malassezia isoladas. MATERIAL E MÉTODOS: Cento e dois pacientes com diagnóstico clínico e laboratorial de pitiríase versicolor foram acompanhados por um período de 12 meses para observarmos o número de recidivas da doença. RESULTADOS: A pitiríase versicolor, após um tratamento adequado, apresentou três tipos de evolução clínica num período de 12 meses: pitiríase versicolor sem nenhum episódio de recidiva (32,35%; pitiríase versicolor recidivante, com um a quatro episódios de recidiva (52,94% devidos a fatores de predisposição relacionados; e pitiríase versicolor crônica, com mais de quatro episódios de recidiva (14,70% sem nenhuma relação com fatores de predisposição. CONCLUSÕES: A pitiríase versicolor apresentou uma evolução clínica de acordo com o número de episódios de recidiva da doença analisados durante um período de 12 meses que pode ser considerada da seguinte maneira: pitiríase versicolor com cura clínica e micológica, pitiríase versicolor recidivante e pitiríase versicolor crônica.BACKGROUND: Pytiriasis versicolor is an infectious disease caused by several Malassezia species which has a tendency to become relapsing or chronic. OBJECTIVES: This study was conducted in an attempt to investigate the clinical course of pityriasis versicolor with regard to the number of relapses after a 12-month therapy and correlate this number with isolates of Malassezia species. MATERIAL AND METHODS: 102 patients with clinical and laboratory diagnosis of pityriasis versicolor were monitored for 12

  14. Predisposing factors on the surface of the skin in persons with pityriasis versicolor.

    Science.gov (United States)

    Gloor, M; Kümpel, D; Friederich, H C

    1975-12-31

    This is a report on biochemical and physiological examinations carried out on 20 test persons who in the last 2 years had contacted and completely recovered from pityriasis versicolor and on 25 control persons of corresponding age and sex. The tests on the skin surface of the patients with pityriasis versicolor show, when compared with the corresponding control group, the following significant results: 1. Significantly more amino acids could be extracted from the skin of the pityriasis versicolor patients than from the skin of the control persons. 2. A significantly shorter alkali neutralisation time was to be found in the pityriasis versicolor patients than in the control persons. 3. The degree of water spreading on the skin was found to be significantly reduced in the pityriasis versicolor patients when compared with the control persons. It is probable that these results point to important predisposing factors for pityriasis versicolor.

  15. Production of sterigmatocystin by Aspergillus versicolor and Bipolaris sorokiniana on semisynthetic liquid and solid media.

    Science.gov (United States)

    Rabie, C J; Lubben, A; Steyn, M

    1976-08-01

    Higher yields of sterigmatocystin were obtained with Aspergillus versicolor than with Bipolaris sorokiniana both in liquid and on solid media. The optimum temperature for sterigmatocystin production by A. versicolor was 27 to 29 degrees C and 23 degrees C for B. sorokiniana. In liquid shake cultures, production of sterigmatocystin by B. sorokiniana was negligible, whereas maximal production by A. versicolor was 210 mg/liter. On solid substrates, the highest yields (8 g/kg) were obtained with A. versicolor on still cultures of whole corn supplemented with Soytone.

  16. Pityriasis versicolor on penile shaft in a renal transplant recipient.

    Science.gov (United States)

    Ryu, Han-Won; Cho, Jae-We; Lee, Kyu-Suk

    2012-08-01

    Pityriasis versicolor is a superficial infection of the stratum corneum, which is caused by the Malassezia species. Tge Malassezia species consist of 12 subspecies, including M. furfur, M. pachydermatis, M. symphodialis and M. globasa. The Malassezia species are classified as a normal flora, particularly in the sebum rich areas of the skin, and they convert from saprophytic yeast to parasitic mycelial morpholgic form to cause clinical disease. But majorities of their distributions are in the upper back, the neck, the thighs, and the forearm, and not in the penis. It is well known that the renal transplant patients, who take immunosuppressive agents, have impairment in the protective cell mediated immunity. Thus, they are more susceptible to infectious diseases, such as a fungal infection. Therefore, clinical manifestations show higher incidence of disease, but they mostly occur in an expected distribution. We here report a case of pityriasis versicolor in a renal transplant recipient on penile shaft, which is an unusual area.

  17. A Case of Confluent and Reticulated Papillomatosis Mimicking Pitriasis versicolor

    Directory of Open Access Journals (Sweden)

    Gürlevik Z et al.

    2012-05-01

    Full Text Available Confluent and reticulated papillomatosis (CRP which is also known as Gourgerot-Corteoud Syndrome is an unusual condition. The etio-pathogenesis is still unclear. This disease is mostly affecting female gender. The most common location for the CRP is on the intermammary and interscapular areas. We presented the case of a male patient with CRP resembling Pityriasis versicolor. The patient was treated with azitromycin and topical retinoid. We observed a good response to azitromycin.

  18. Comparative histopathology of Pityriasis versicolor and Pityrosporum folliculitis

    Directory of Open Access Journals (Sweden)

    Mittal R

    1992-01-01

    Full Text Available Twenty five cases each of pityriasis versicolor (PV and pityrosporum folliculitis (PF were selected for this study. Histopathologically in PV, spores and hyphae in spaghetti and meatball appearance in stratum corneum, and perivascular mononuclear infiltrate were seen. In PF, spores were inside the dilated hair follicles filled with keratin, perifollicular and perivascular mononuclear infiltration were seen. Basket weave hyperkeratosis, edema and disruption of collagen were seen in 88% cases of PF and have not been reported earlier.

  19. Four Novel Hydropyranoindeno- Derivatives from Marine Fungus Aspergillus versicolor

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    From the cultured filtrates of fungus Aspergillus versicolor,isolated from marine sponge Xestospongia exigua,four novel secondary metabolites,namely aspergillone 1,aspergillodiol 2, aspergillol 3 and 12-acetyl-aspergillol 4,have been isolated by column chromatographic separation.The structures of all the new compounds are established on the basis of extensive 2D NMR spectroscopy in conjugation with MS,UV spectral analysis.The basic structure pattern of those compounds possessed an hydroindenoisopyran nucleus.

  20. [Pityriasis versicolor--modern views on etiology, pathogenesis and therapy].

    Science.gov (United States)

    Karadaglić, Dj

    1992-01-01

    Pityriasis versicolor (Tinea versicolor) is a superficial chronic fungal infection caused by Pityrisporum species which are normal "inhabitants" of the cutaneous flora. The morphologic changes from yeast to mycelial hypha form are important in the development of clinical lesions. The onset and course of the disease are under the influence of genetic factors, age, sex, climate, local environmental factors, malnutrition, pregnancy, oral contraceptives, corticosteroid and immunosuppressive treatment. They favorize transformation of saphrophytic to pathogenic form, and are the cause of recurrences and chronicity of the disease. The ultrastructural and immunologic studies which are carried out today would significantly contribute to a better understanding of pathogenesis of the disease. The disease is limited to seborrheic areas of the skin and commonly has three clinical forms: papulosquamous, follicular and inverse. This can make great problems in differential diagnosis. In the treatment of these patients it should be kept in mind that Pityriasis versicolor is not a primary contagious disease and that conversion of Pityrisporum depends on the predisposing factors. Numerous medicaments with local and systemic effect which are used nowaday in the treatment and prevention of pityriasis are reported. Way of application, doses, duration of therapy, advantages and disadvantages are given in detail. PMID:1465674

  1. Adult Diapause in Coleoptera

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    Ivo Hodek

    2012-01-01

    Full Text Available Recent studies dealing with adult (reproductive diapause in the Coleoptera are reviewed, as a kind of supplement to the classic compendia. In the first two sections, the general characteristics of adult diapause are described and principal terms explained. Original articles dealing with 19 species from nine coleopteran families (Coccinellidae, Chrysomelidae, Bruchidae, Curculionidae, Carabidae, Silphidae, Scolytidae, Scarabaeidae, and Endomychidae are reviewed. Finally attempts are made at generalisations from the papers reviewed, and hypotheses on diapause evolution are inferred. A polyphenic character of diapause is a prominent feature in C. septempunctata and L. decemlineata, but has been found also in other Coleoptera and in insects generally and often generates voltinism heterogeneity within populations.

  2. Follicular pityriasis versicolor-Rare variant of a common dermatological disease

    Directory of Open Access Journals (Sweden)

    Tasleem Arif

    2015-01-01

    Full Text Available Pityriasis versicolor is a superficial fungal infection of the skin caused by yeast of the genus Malassezia. It is characterized by hypopigmented or hyperpigmented macules and patches usually involving the trunk [1]. The variants of pityriasis versicolor include hypochromic, hyperchromic, combination of hypo-hyperchromic, erythematous, circinate, atrophic and acral [2-4].

  3. Clinical Trial Of Ketoconazole 2% + Zinc Pyrithione 1% Shampoo In The Management Of Tinea Versicolor

    Directory of Open Access Journals (Sweden)

    Ramesh M

    2004-01-01

    Full Text Available An open uncontrolled trial, to assess the efficacy of ketoconazole 2%+zinc pyrithione 1% shampoo was conducted in patients with extensive tinea versicolor. The results show that this is an effective and well tolerated combination in the management of tinea versicolor.

  4. Структура комплексов жужелиц (Insecta: Coleoptera: Carabidae) плантационных посадок брусники в условиях Беларуси в первые годы после закладки

    OpenAIRE

    Буга, С. В.; О.Р. Александрович; Морозов, О. В.

    2013-01-01

    Species composition and ecological structure of carabid beetles (Insecta: Coleoptera: Carabidae) community at cowberry plantations were studied in SW Belarus in the first years after planting. Terrestrial invertebrates were sampled by pitfall traps. A total of 3028 imagines of 45 carabid species have been collected. Activity density of carabid beetles was the highest from late May to early June. Poecilus versicolor (Sturm, 1824) was the most abundant (87,39 %). Species richness of predator and...

  5. Tinea versicolor, tinea nigra, white piedra, and black piedra.

    Science.gov (United States)

    Bonifaz, Alexandro; Gómez-Daza, Fernando; Paredes, Vanessa; Ponce, Rosa María

    2010-03-01

    Superficial mycoses are fungal infections limited to the stratum corneum and its adnexal structures. The most frequent types are dermatophytoses or tineas. Tinea versicolor involves the skin in the form of hypochromic or hyperchromic plaques, and tinea nigra affects the skin of the palms with dark plaques. White piedra and black piedra are parasitic infections of scalp hairs in the form of concretions caused by fungal growth. Diagnosis of these mycoses is made from mycologic studies, direct examination, stains, and isolation, and identification of the fungi. Treatment includes systemic antifungals, topical antifungals, and keratolytics.

  6. Identification of Malassezia yeast species isolated from patients with pityriasis versicolor.

    Science.gov (United States)

    Petry, Vanessa; Tanhausen, Fernanda; Weiss, Luciana; Milan, Thais; Mezzari, Adelina; Weber, Magda Blessmann

    2011-01-01

    Pityriasis versicolor (PV) is a disease with worldwide distribution. Twelve different species of Malassezia yeast have been described. The objective of this study was to determine which species of Malassezia are more prevalent in patients with pityriasis versicolor. Samples were collected by scraping the lesions of 87 patients with a clinical suspicion of pityriasis versicolor. The samples were then submitted to fungal microscopy and culture to identify the species. The species found were: Malassezia sympodialis (30%), Malassezia furfur (25.7%), Malassezia globosa (22.7%), Malassezia restricta (12.1%), Malassezia obtusa (7.6%) and Malassezia slooffiae (1.5%).

  7. Структура комплексов жужелиц (Insecta: Coleoptera: Carabidae) плантационных посадок голубики высокорослой в условиях Беларуси в первые годы после закладки

    OpenAIRE

    Буга, С. В.; О.Р. Александрович

    2013-01-01

    Species composition and ecological structure of carabid beetles (Insecta: Coleoptera: Carabidae) communities at highbush blueberry plantations were studied in SW Belarus in the first years after planting. Terrestrial invertebrates were sampled by pitfall traps. A total of 623 imagines of 51 carabid species have been collected. Field and meadow mesophilous and mesokserophilous species are formed main part of whole carabid complex. Poecilus versicolor (Sturm, 1824) and Agonum sexpunctatum (...

  8. [Labor epidural analgesia for a woman with a pityriasis versicolor in the lumbar region].

    Science.gov (United States)

    Dubar, G; Omarjee, M; Viguié, C; Barbarot, S; Mignon, A

    2011-01-01

    Epidural analgesia is usually contraindicated in case of infection at the site of needle insertion. Tinea versicolor is a benign superficial cutaneous fungal infection caused by the proliferation of a skin commensal yeast of low pathogenicity. We report the case of a pregnant woman with a tinea versicolor in the lumbar region, who benefited from a labor epidural analgesia, realised with reinforced antiseptic measures. No neurological or infectious complication occurred.

  9. Statistical optimization for decolorization of textile dyes using Trametes versicolor.

    Science.gov (United States)

    Srinivasan, S V; Murthy, D V S

    2009-06-15

    The conventional treatment of dark coloured textile wastewater using chemical coagulation generates large volume of sludge, which requires further treatment and disposal. In the present investigation, a systematic optimization study of the important variables influencing the decolorization of Reactive Orange-16 (RO-16) and Reactive Red-35 (RR-35) dyes by the white-rot fungus (Trametes versicolor) was carried out. A full factorial central composite design was employed for experimental design and optimization of results. The effect of concentrations of dye, glucose and ammonium chloride on decolorization was studied and optimized using Response Surface Methodology (RSM). Maximum decolorization of 94.5% and 90.7% for RO-16 and RR-35 was obtained at optimum concentrations of dye, glucose and ammonium chloride i.e., 0.66, 17.50 and 2.69 g/L for RO-16 and 0.68, 16.67 and 2.13 g/L for RR-35, respectively.

  10. Immunological study of Pityriasis versicolor and Pityrosporum folliculitis

    Directory of Open Access Journals (Sweden)

    Mittal R

    1992-01-01

    Full Text Available Twenty five cases of pityriasis versicolor and 25 cases of pityrosporum folliculitis were selected for this study. Skin scrapings in 10% potassium hydroxide showed spores and hyphae in all 25 cases of PV. Smears from PF showed Gram positive spores in all 25 cases and in addition few short hyphae in 3 cases. Initially IgM and Later both IgM and IgG were raised. Similar response was seen in PF initially and later IgM, IgG and IgA all were raised in PF. Though antibody levels were slightly higher in PF, yet basically the pattern was similar in PV and PF.

  11. Study of oral Ketoconazole on recurrent & extensive Pityriasis Versicolor

    Directory of Open Access Journals (Sweden)

    Moghaddami M

    1997-04-01

    Full Text Available Several topical agents have been used for treating Pityriasis versicolor. But the main problem is recurrence. In this study 82 patients with recurrent and/or extensive from of infection were treated with oral Ketoconazole. 200 mg once daily for a maximum of 4 weeks. Seventeen patients have been infected for the first time and sixty five for the several times. The duration of disease in most cases (35.4% were between 1-3 years. Out of 82 studied patients only 60 cases were referred for follow up. The clinical and mycological cure were seen in 54 cases (90% after 15 days therapy. In other 6 patients after 15 days the lesions have not cured completely so the treatment was continued for other 2 weeks, but after that, only one of them referred to the clinic, which treatment extended with topical therapy.

  12. Effects of catalysts on liquefaction of Agaricus versicolor (L.)

    Science.gov (United States)

    Durak, Halil

    2016-04-01

    Supercritical liquefaction process is used for producing energy from biomass. The common reaction conditions for supercritical liquefaction process are the 240-380 °C temperature range and 5-20 Mpa pressure values range. Agaricus versicolor (L.) was liquefied by acetone in an autoclave (75 mL) under high pressure with (aluminium oxide and calcium hydroxide) and without catalyst at 290 °C for producing bio-oil. The products of liquefaction (bio-oil) were analysed and characterized using various methods including elemental analysis, Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. GC-MS identified 27 different compounds in the bio-oils obtained at 290 °C.

  13. 1825 (Coleoptera: Cerambycidae

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    Márcia d´Avila

    2006-01-01

    Full Text Available The erva-mate (Ilex paraguariensis A.St.-Hil. has a social, cultural and economic importance in the southern states of Brazil. The pure stands of this culture was responsible for the increase of many species of insects. Hedypathes betulinus (Klug, 1825 (Coleoptera: Cerambycidae is considered the main pest from an economic viewpoint because of its difficult control and potential for damage. The larval phase occurs inside the twings and trunks, what makes more difficult to deal with its detention and management. Cultural and mechanical management are the most indicated, such as collection of adults, prunning and burning of plant parts damaged by the insect, balanced nutrition, adequate plant density and maintenance of areas with native vegetation or also the introduction of policulture. These strategies may increase the agroecossystem balance and thus a reduction of the insect-pest to an aceptable level. Therefore, the objective of this study was to assemble and the discuss the information on the bioecology and management of erva-mate borer.

  14. Pityriasis versicolor: A clinicomycological and epidemiological study from a tertiary care hospital

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    Ghosh Sudip

    2008-01-01

    Full Text Available Background: Pityriasis versicolor is a mild, chronic, usually asymptomatic superficial fungal infection of the stratum corneum, caused by Malassezia yeasts. The purpose of the present study is to assess the clinical profile of a group of patients with pityriasis versicolor and to find out the epidemiological characteristics in this part of India as well as any association, if any, with other diseases. Materials and Methods: For this purpose, 110 consecutive patients of pityriasis versicolor were evaluated clinically and diagnosis was confirmed mycologically at a tertiary care hospital in Kolkata. All data were recorded in a predesigned, pretested semi-structured schedule. The total duration of study period was 12 months. Results: Majority of the patients were young adults. Most of the patients were asymptomatic. There is prominent seasonal variation of the patients with a peak in August and September months. Most of the lesions were hypopigmented scaly macules and were KOH positive. Most commonly involved sites were chest, face and back. Seborrheic dermatitis sometimes coexisted with pityriasis versicolor and a number of patients also had diabetes mellitus and immunosuppressive conditions. Conclusions: Overall, the clinicomycological and epidemiological profile of pityriasis versicolor infection as observed in a tertiary care setting in eastern India does not differ significantly from those observed by previous workers elsewhere.

  15. Isolation of Malassezia globosa and M. sympodialis from patients with pityriasis versicolor in Spain.

    Science.gov (United States)

    Aspiroz, Carmen; Ara, Mariano; Varea, Marzo; Rezusta, Antonio; Rubio, Carmen

    2002-01-01

    Pityriasis versicolor is a superficial infection of the stratum corneum by several yeast species formerly collectively named Malasseziafurfur. The genus Malassezia has been recently enlarged with new species. With the exception of M. pachydermatis, the remaining six species have an absolute requirement in vitro for supplementation of long-chain fatty acids in media. These lipophilic yeasts comprise six species: M. furfur, M. globosa, M. obtusa, M. restricta, M. slooffiae and M. sympodialis. The aim of this study was to establish whether there was any association between the various species of Malassezia and pityriasis versicolor lesions. Thus, we studied the isolates from 79 patients with pityriasis versicolor, both from lesions and from apparently healthy skin close to the visible lesions. In pityriasis versicolor lesions, the species most frequently isolated was M. globosa (90%), followed by M. sympodialis (41%). Almost all isolates (99%) belonged to one of these two species. The most frequent pattern was M. globosa as the sole species (58% of cases), although the association with M. sympodialis was also frequent (30%). These results confirmed M. globosa as the main agent of pityriasis versicolor and M. sympodialis as the second agent in importance. Malassezia globosa was found to be a species with high levels of esterase and lipase enzymes of probable importance in their pathogenicity.

  16. Bioremediation of zirconium from aqueous solution by coriolus versicolor: process optimization

    International Nuclear Information System (INIS)

    In the present study the potential of live mycelia of Coriolus versicolor was explored for the removal of zirconium from simulated aqueous solution. Optimum experimental parameters for the bioremediation of zirconium using C. versicolor biomass have been investigated by studying the effect of mycelia dose, concentration of zirconium, contact time and temperature. The isothermal studies indicated that the ongoing bioremediation process was exothermic in nature and obeyed Langmuir adsorption isotherm model. The Gibbs free energy (ΔG), entropy (ΔS) and enthalpy (ΔH) of bioremediation were also determined. The result showed that bioremediation of zirconium by live C. versicolor was feasible and spontaneous at room temperature. The equilibrium data verified the involvement of chemisorption during the bioremediation. The kinetic data indicated the operation of pseudo-second order process during the biosorption of zirconium from aqueous solution. Maximum bioremediation capacity (110.75 mg/g) of C. versicolor was observed under optimum operational conditions: pH 4.5, biomass dose 0.05 mg/100 mL, contact time 6 h and temperature 30 degree C. The results showed that C. versicolor could be used for bioremediation of heavy metal ions from aqueous systems. (author)

  17. Occurrence of the Toxin-Producing Aspergillus versicolor Tiraboschi in Residential Buildings.

    Science.gov (United States)

    Piontek, Marlena; Łuszczyńska, Katarzyna; Lechów, Hanna

    2016-01-01

    In an area representative of a moderate climate zone (Lubuskie Province in Poland), mycological tests in over 270 flats demonstrated the occurrence of 82 species of moulds. Aspergillus versicolor Tiraboschi was often encountered on building partitions (frequency 4: frequently). The ability to synthesize the carcinogenic sterigmatocystin (ST) means that it poses a risk to humans and animals. Biotoxicological tests of biomasses of A. versicolor were conducted in the Microbiological and Toxicological Laboratory, using the planarians Dugesia tigrina (Girard). The obtained results of the tests covered a broad range of toxicity levels of isolated strains: from weakly toxic (100-1000 mg·L(-3)) to potently toxic (1-10 mg·L(-3)). The high-performance liquid chromatography (HPLC) physicochemical method confirmed the ability of A. versicolor strains to synthesize sterigmatocystin. All of the samples of the air-dry biomasses of the fungi contained ST in the range between 0.03 and 534.38 mg·kg(-1). In the bio-safety level (BSL) classification A. versicolor belongs to category 1. Additionally, A. versicolor is an allergenic mould. PMID:27589778

  18. Removal of monomer delignification products by laccase from Trametes versicolor.

    Science.gov (United States)

    Kolb, Michaela; Sieber, Volker; Amann, Manfred; Faulstich, Martin; Schieder, Doris

    2012-01-01

    The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5 U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24h. However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase. Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1 day (LRG-B), no effect of laccase (LRG-C). Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions.

  19. [Pityriasis versicolor : new aspects of an old disease].

    Science.gov (United States)

    Mayser, P A; Preuss, J

    2012-11-01

    Pityriasis versicolor (PV) is one of the most common infectious skin diseases, as well as the most common dermatosis associated with pigmentation alterations of the skin. PV is prevalent in 1% of the population living in temperate climate zones and more common during the summer. In tropical areas, PV is found in up to 50% of all patients consulting a dermatologist. Of the known Malassezia species, M. globosa is currently felt to play a key role in the pathogenesis of PV, as it is most commonly found in PV lesions. In addition, its round-shaped cells may contribute to the characteristic histology of the disease ("spaghetti and meatballs"). However, the clinical appearance of PV including hyper- and hypopigmentation, fluorescence of the lesions, as well as a lack of inflammation despite high fungal load cannot fully be explained by the presence of M. globosa, which is also found on healthy skin. In M. furfur a tryptophan-dependent metabolic pathway generates a number of indole pigments, which may be associated with the clinical appearance of PV. In the model organism Ustilago maydis it was shown that the formation of the indole compounds occurs spontaneously after initial conversion of tryptophan into indole pyruvate controlled by the key enzyme aminotransferase Tam 1. We review the present knowledge of PV and highlight the potential role of Tam1 in explaining the poorly understood aspects of the disease. Promising therapeutic results using the application of Tam1 inhibitors to treat PV support the enzyme's important role in the disease pathogenesis.

  20. Corn forage biological pretreatment by Trametes versicolor in a tray bioreactor.

    Science.gov (United States)

    Planinić, Mirela; Zelić, Bruno; Čubel, Ivan; Bucić-Kojić, Ana; Tišma, Marina

    2016-08-01

    Trametes versicolor is a white-rot fungus known to be efficient in lignin removal due to its complex extracellular lignocellulolytic enzymatic system. Therefore, it can be used in the treatment of lignocellulose waste from agro, food, and wood industries. In a first experiment, corn forage treatment with T. versicolor was investigated in laboratory jars. In a second experiment, the process was scaled up to a tray bioreactor. In the tray bioreactor, the process of lignin degradation was improved, resulting in an increase in lignin conversion of up to 71% during seven days' treatment. PMID:27401159

  1. Pitiríase versicolor circinada: isolamento de Malassezia sympodialis - Relato de caso Pityriasis versicolor circinata: isolation of Malassezia sympodialis - Case report

    Directory of Open Access Journals (Sweden)

    Valéria Maria de Souza Framil

    2010-04-01

    Full Text Available Os autores descrevem caso de pitiríase versicolor circinada, cujo agente etiológico isolado foi Malassezia sympodialis em uma mulher de 34 anos. O isolamento e identificação da Malassezia sympodialis foi em ágar Dixon modificado e o método molecular para confirmação da espécie foi PCR-RFLP (polymerase chain reaction and restriction fragment length polymorphism analysis.The authors report a case of pityriasis versicolor circinata whose isolated etiologic agent was Malassezia sympodialis in a 34-year-old woman. The isolation and identification of Malassezia sympodialis were accomplished with modified Dixon's agar, and the molecular method used to confirm the species was polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP.

  2. SIXTEEN YEARS OF PITYRIASIS VERSICOLOR IN METROPOLITAN AREA OF PORTO ALEGRE, SOUTHERN BRAZIL.

    Science.gov (United States)

    Heidrich, Daiane; Daboit, Tatiane Caroline; Stopiglia, Cheila Denise Ottonelli; Magagnin, Cibele Massotti; Vetoratto, Gerson; Amaro, Taís Guarienti; Scroferneker, Maria Lúcia

    2015-01-01

    Pityriasis versicolor is the most common of the diseases caused by Malassezia yeasts. The aim of this study is to determine the prevalence of pityriasis versicolor and its etiological aspects in the metropolitan area of Porto Alegre, Brazil. A retrospective cross-sectional study with data from patients of a reference hospital from 1996 to 2011 was performed. Collected data included: date, age, gender, ethnicity, anatomical region of lesion and the direct mycological examination results. Among the positive results in the direct mycological examination, 5.8% (2,239) were positive for pityriasis versicolor. The angular coefficient (B) was -0.3%/year, showing a decrease over the years. The disease was more prevalent in men (7.1% of men versus 5.1% of women that underwent the direct mycological examination); younger age (median 31 years old); "pardo" and black people (3.7% more than expected in the sample); trunk (73.44% of the affected anatomic sites). Lesions in rare sites (groin, genitals, legs, feet and hands) were also observed in this study. In conclusion, due to the decrease in the prevalence of pityriasis versicolor, long-term epidemiological studies in the metropolitan area of Porto Alegre, Brazil, are needed to continue the monitoring of this disease.

  3. Hypopigmented pityriasis versicolor on Becker′s naevus : Hope for new method of treatment ?

    Directory of Open Access Journals (Sweden)

    Singal Archana

    1998-01-01

    Full Text Available A case of hypopigmented pityriasis versicolor superimposed on pre existing Becker′s naevus associated with congenital melanocytic naevus is being reported for its rarity. The possible role of dicarboxylic acid and other free radicles produced by Pityrosporum ovale in treating Becker′s Naevus is also suggested.

  4. Pityriasis versicolor: clinical experience with Lamisil cream and Lamisil DermGel.

    Science.gov (United States)

    Faergemann, J; Hersle, K; Nordin, P

    1997-01-01

    Pityriasis versicolor is a chronic superficial fungal disease usually located on the upper trunk, neck or upper arms. Terbinafine is an orally and topically active allylamine antifungal derivative with a broad antifungal spectrum. Several studies have documented the high activity of terbinafine (Lamisil) cream in the treatment of pityriasis versicolor, with cure rates of 79-100% after 2-4 weeks of treatment. Lamisil emulsion gel (Lamisil DermGel) is a new topical formulation that remains in the skin in high concentrations for several months after just 1 week of application. Recently, in a prospective, double-blind, placebo-controlled, randomised, parallel-group, comparative, multicentre study, we have treated patients with pityriasis versicolor with either terbinafine 1% emulsion gel (Lamisil DermGel) or placebo gel. Sixty-one patients were included, 31 in the Lamisil DermGel group and 30 in the placebo gel group. The gel was applied once daily for 7 days and the patients were followed up for 8 weeks. Twenty-eight patients in the active and 29 patients in the placebo group were evaluable for efficacy. Using the intent-to-treat efficacy analysis, 21/28 (75%) were cured in the Lamisil-DermGel-treated group compared to 4/29 (14%) in the placebo group. No side-effects were seen. In conclusion, Lamisil DermGel was well tolerated and superior to placebo in the treatment of pityriasis versicolor when applied once daily for 7 days.

  5. Potential of extracellular enzymes from Trametes versicolor F21a in Microcystis spp. degradation.

    Science.gov (United States)

    Du, Jingjing; Pu, Gaozhong; Shao, Chen; Cheng, Shujun; Cai, Ji; Zhou, Liang; Jia, Yong; Tian, Xingjun

    2015-03-01

    Studies have shown that microorganisms may be used to eliminate cyanobacteria in aquatic environments. The present study showed that the white-rot fungus Trametes versicolor F21a could degrade Microcystis aeruginosa. After T. versicolor F21a and Microcystis spp. were co-incubated for 60h, >96% of Microcystis spp. cells were degraded by T. versicolor F21a. The activities of extracellular enzymes showed that cellulase, β-glucosidase, protease, and laccase were vital to Microcystis spp. degradation in the early stage (0h to 24h), while β-glucosidase, protease, laccase, and manganese peroxidase in the late stage (24h to 60h). The positive and significant correlation of the degradation rate with these enzyme activities indicated that these enzymes were involved in the degradation rate of Microcystis spp. cells at different phases. It suggested that the extracellular enzymes released by T. versicolor F21a might be vital to Microcystis spp. degradation. The results of this study may be used to develop alternative microbial control agents for cyanobacterial control.

  6. DISTRIBUTION OF MALASSEZIA SPECIES IN PATIENTS WITH PITYRIASIS VERSICOLOR AND HEALTHY INDIVIDUALS IN SOUTH INDIA

    Directory of Open Access Journals (Sweden)

    Kavitha

    2016-04-01

    Full Text Available BACKGROUND Pityriasis Versicolor is a superficial infection of the stratum corneum which is caused by a group of yeasts called Malassezia. OBJECTIVES To isolate and identify the causative fungi from clinically suspected cases of Pityriasis Versicolor by 10% KOH and to compare the results of 10% KOH with culture. METHODS 100 clinically diagnosed cases of Pityriasis Versicolor of all age groups and both sexes, attending the Outpatient Department of Dermatology at Bapuji Hospital and Chitageri Hospital were included in this study. Skin scrapings from lesions of all cases were subjected to 10% KOH examination and culture. A control group of 50 healthy individuals was studied to know the normal fungal flora of the skin. Differentiation of all Malassezia species performed using morphological features and physiological tests including catalase reaction, Tween assimilation test and splitting of esculin. RESULTS Out of 100 clinically diagnosed Pityriasis Versicolor cases, 97(97% cases showed characteristic arrangement of fungal elements in 10% KOH preparation and culture was positive in 68 cases. The most frequently isolated species was M. globosa (54.41%, followed by M. furfur (25%, M. sympodialis (11.76% and M. obtusa (8.82%. In control group, M. globosa was the common isolate. CONCLUSION In comparison with culture results, the sensitivity and specificity of 10% KOH is 100% and 9.37% respectively. It does not require highly skilled personnel to perform or interpret results. The only disadvantage of KOH examination is that the Malassezia species cannot be differentiated. Culture is the gold standard, but it is time consuming and requires considerable expertise. Therefore, 10% KOH examination is a rapid, simple, sensitive and effective diagnostic test for Pityriasis Versicolor. M. globosa was the most common isolated species followed by M. furfur, M. sympodialis and M. obtusa

  7. Distribution of Malassezia species in patients with pityriasis versicolor in Turkey.

    Science.gov (United States)

    Rodoplu, G; Saracli, M A; Gümral, R; Taner Yildiran, S

    2014-06-01

    Pityriasis versicolor is a common superficial mycotic disease of the skin which is caused by different species of Malassezia genus. The aim of this study was to contribute to the knowledge of the aetiology of pityriasis versicolor (PV) with a mycological study made according to the new species and additionally, the success of the different sampling techniques, duration and recurrence history of the disease, distribution of infecting strains according to the affected body sites were also investigated. In total, 146 patients with pityriasis versicolor were included in this study. Fungal elements could only be visualized by potassium hydroxide examination in 36.4% of the samples. Specimens obtained by scraping skin surface by a sterile scalpel and/or sterile sticky plaster (OpSite) were inoculated in plates containing modified Dixon's medium. Out of 146 samples, 109 (74.7%) yielded a growth which was considered to be Malassezia spp. in culture. Species level identification of suspicious Malassezia yeasts was made according to their macroscopic and microscopic features, and their physiological characteristics. Among the identified species, Malassezia globosa (65.1%) was the most commonly isolated species, followed by Malassezia obtusa (17.4%). However, four Malassezia isolates could not be identified at species level with conventional methods. While most of the patients suffered their first episode of pityriasis versicolor (76%), back of the trunk was the mostly affected body site (39%). In conclusion, Malassezia globosa was found to be the predominant species in pityriasis versicolor patients in our region, and culture of the specimen is necessary for the epidemiologic purposes.

  8. Identification and speciation of Malassezia in patients clinically suspected of having pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Avani Shah

    2013-01-01

    Full Text Available Background: Malassezia is a lipid-dependent yeast known to cause Pityriasis versicolor, a chronic, recurrent superficial infection of skin and present as hypopigmented or hyperpigmented lesions on areas of skin. If not diagnosed and treated, it may lead to disfigurement of the areas involved and also result in deep invasive infections. Aim: The aim of the present study was to identify and speciate Malassezia in patients clinically suspected of having Pityriasis versicolor. Materials and Methods: Total 139 patients suspected of having Pityriasis versicolor were evaluated clinically and diagnosis was done by Wood′s lamp examination, confirmed mycologically by using KOH, cultivation on Sabouraud′s dextrose agar and modified Dixon agar at a tertiary care hospital in Mumbai. The total duration of study was 12 months. Results: Majority of the patients were males (59.71% in the age group of 21-30 years (33.81% who were students (30.21% by profession. The incidence of Malassezia in Pityriasis versicolor was 50.35%. The most common isolate was M. globosa (48.57%, followed by M. furfur (34.28%. Majority of the patients had hypopigmented lesions, with M. globosa as the predominant isolate. Neck was the most common site affected; 88.48% were Wood′s lamp positive of which 56.91% of Malassezia isolates grew on culture. KOH mount was positive in 82.01% of which 61.40% Malassezia isolates grew on culture. Conclusions: The procedure of culture and antifungal testing is required to be performed as different species of Malassezia are involved in Pityriasis versicolor and susceptibility is different among different species. Thus, it would help to prevent recurrences and any systemic complications.

  9. DESTRUCTION OF ASPERGILLUS VERSICOLOR, PENICILLIUM CRYSOGENUM, STACHYBOTRYS CHARTARUM, AND CLADOSPORIUM CLADOSPORIDES SPORES USING CHEMICAL OXIDATION TREATMENT PROCESS

    Science.gov (United States)

    The survival of aqueous suspensions of Penicillium chrysogenum, Stachybotrys chartarum, Aspergillus versicolor, and Cladosporium cladosporioides spores was evaluated using various combinations of hydrogen peroxide and iron (II) as catalyst. Spores were suspended in water and trea...

  10. Biodegradation and utilization of 4-n-nonylphenol by Aspergillus versicolor as a sole carbon and energy source

    Energy Technology Data Exchange (ETDEWEB)

    Krupiński, Mariusz; Janicki, Tomasz [Department of Industrial Microbiology and Biotechnology, University of Lodz, Banacha 12/16, 90-237 Łódź (Poland); Pałecz, Bartłomiej [Department of Physical Chemistry, University of Lodz, Pomorska 165, 90-236 Łódź (Poland); Długoński, Jerzy, E-mail: jdlugo@biol.uni.lodz.pl [Department of Industrial Microbiology and Biotechnology, University of Lodz, Banacha 12/16, 90-237 Łódź (Poland)

    2014-09-15

    Highlights: • A. versicolor is able to degrade 4-n-NP as the sole source of carbon and energy. • 4-n-NP removal by A. versicolor was accompanied by the formation of metabolites. • Radioactive experiments show complete 4-n-NP mineralization by A. versicolor. • 4-n-NP initiates heat production in the A. versicolor spores. - Abstract: 4-n-Nonylphenol (4-n-NP) is an environmental pollutant with endocrine-disrupting activities that is formed during the degradation of nonylphenol polyethoxylates, which are widely used as surfactants. Utilization of 4-n-NP by the filamentous fungus Aspergillus versicolor as the sole carbon and energy source was investigated. By means of gas chromatography–mass spectrometry, we showed that in the absence of any carbon source other than 4-n-NP in the medium, A. versicolor completely removed the xenobiotic (100 mg L{sup −1}) after 3 d of cultivation. Moreover, mass spectrometric analysis of intracellular extracts led to the identification of eight intermediates. The mineralization of the xenobiotic in cultures supplemented with 4-n-NP [ring-{sup 14}C(U)] as a growth substrate was also assessed. After 3 d of incubation, approximately 50% of the initially applied radioactivity was recovered in the form of {sup 14}CO{sub 2}, proving that this xenobiotic was completely metabolized and utilized by A. versicolor as a carbon source. Based on microscopic analysis, A. versicolor is capable of germinating spores under such conditions. To confirm these observations, a microcalorimetric method was used. The results show that even the highest amount of 4-n-NP initiates heat production in the fungal samples, proving that metabolic processes were affected by the use of 4-n-NP as an energetic substrate.

  11. Biodegradation and utilization of 4-n-nonylphenol by Aspergillus versicolor as a sole carbon and energy source

    International Nuclear Information System (INIS)

    Highlights: • A. versicolor is able to degrade 4-n-NP as the sole source of carbon and energy. • 4-n-NP removal by A. versicolor was accompanied by the formation of metabolites. • Radioactive experiments show complete 4-n-NP mineralization by A. versicolor. • 4-n-NP initiates heat production in the A. versicolor spores. - Abstract: 4-n-Nonylphenol (4-n-NP) is an environmental pollutant with endocrine-disrupting activities that is formed during the degradation of nonylphenol polyethoxylates, which are widely used as surfactants. Utilization of 4-n-NP by the filamentous fungus Aspergillus versicolor as the sole carbon and energy source was investigated. By means of gas chromatography–mass spectrometry, we showed that in the absence of any carbon source other than 4-n-NP in the medium, A. versicolor completely removed the xenobiotic (100 mg L−1) after 3 d of cultivation. Moreover, mass spectrometric analysis of intracellular extracts led to the identification of eight intermediates. The mineralization of the xenobiotic in cultures supplemented with 4-n-NP [ring-14C(U)] as a growth substrate was also assessed. After 3 d of incubation, approximately 50% of the initially applied radioactivity was recovered in the form of 14CO2, proving that this xenobiotic was completely metabolized and utilized by A. versicolor as a carbon source. Based on microscopic analysis, A. versicolor is capable of germinating spores under such conditions. To confirm these observations, a microcalorimetric method was used. The results show that even the highest amount of 4-n-NP initiates heat production in the fungal samples, proving that metabolic processes were affected by the use of 4-n-NP as an energetic substrate

  12. Comparative study of oral and topical ketoconazole therapy in pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Nagpal V

    2003-07-01

    Full Text Available Introduction: Both topical and systemic ketoconazole are reported to be effective against pityriasis versicolor. Material and Methods: Forty patients suffering from pityriasis versicolor were treated either with oral ketoconazole 200 mg per day or 2% ketoconazole cream topically once daily for 2 weeks. Results: On global assessment, after 2 weeks of start of therapy, 18 (90% out of 20 patients treated with oral ketoconazole were cured while 2 patients had considerable residual disease. In the ketoconazole cream group, 16 (80% out of 20 patients were cured and 4 patients had considerable residual disease. Conclusion: No significant difference was observed in the response rates in the two groups. Relapse occurred in two patients of the systemic ketoconazole group and six patients of the topical ketoconazole group during the follow-up period of three months.

  13. Role of Laccase and Low Molecular Weight Metabolites from Trametes versicolor in Dye Decolorization

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    Diego Moldes

    2012-01-01

    Full Text Available The studies regarding decolorization of dyes by laccase may not only inform about the possible application of this enzyme for environmental purposes, but also may provide important information about its reaction mechanism and the influence of several factors that could be involved. In this paper, decolorization of crystal violet and phenol red was carried out with different fractions of extracellular liquids from Trametes versicolor cultures, in order to describe the role of laccase in this reaction. Moreover, the possible role of the low molecular weight metabolites (LMWMs also produced by the fungus was evaluated. The results confirm the existence of a nonenzymatic decolorization factor, since the nonprotein fraction of the extracellular liquids from cultures of T. versicolor has shown decolorization capability. Several experiments were performed in order to identify the main compounds related to this ability, which are probably low molecular weight peroxide compounds.

  14. Clinico-epidemiological study of pityriasis versicolor in a rural tertiary care hospital

    Directory of Open Access Journals (Sweden)

    Nazeema Tabaseera

    2014-08-01

    Full Text Available Background: The study was conducted to know the incidence of pityriasis versicolor in relation to age, sex, seasons and occupation in a rural set up. Methods: A total of 105 patients of pityriasis versicolor were clinically evaluated and confirmed mycologically at central lab in a tertiary care hospital in South India. The obtained data was recorded and analysed accordingly. Results: Male preponderance was observed in the study. Disease was most pronounced in younger age group (21-30 years especially students. 61.9% of patients sought medical advice on cosmetic grounds while 38% had pruritis. Chest was the commonest site of affection followed by neck and back. Conclusion: Early identification of the yeast by simple laboratory techniques would help prevent recurrences, systemic complications and cosmetological problems which are high especially among the students and younger age groups. [Int J Res Med Sci 2014; 2(4.000: 1438-1440

  15. Over production of lignocellulosic enzymes of Coriolus versicolor by genetic engineering methodology. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Williams, A.L.

    1998-07-01

    The project seeks to understand the biological and chemical processes involved in the secretion of the enzyme polyphenol oxidase (PPO) by the hyphae, the basic unit of the filamentous fungus Coriolus versicolor. These studies are made to determine rational strategies for enhanced secretion of PPO, both with the use of recombinant DNA techniques and without. This effort focuses on recombinant DNA techniques to enhance enzyme production. The major thrust of this project was two-fold: to mass produce C. versicolor tyrosinase (polyphenol oxidase) by genetic engineering as well as cultural manipulations; and to utilize PPO as a biocatalyst in the processing of lignocellulose as a renewable energy resource. In this study, the assessment of genomic and cDNA recombinant clones with regards to the overproduction of PPO continued. Further, immunocytochemical techniques were employed to assess the mechanism(s) involved in the secretion of PPO by the hyphae. Also, factors influencing PPO secretion were examined.

  16. GC-MS and spectrophotometric analysis of biodegradation of new disazo dye by Trametes versicolor.

    Science.gov (United States)

    Akdogan, H Ardag; Demircali, A; Aydemir, C; Pazarlioglu, N; Karci, F

    2011-01-01

    In this study; sub-tropical white rot fungi, Trametes versicolor was investigated for its ability to degrade 4-(3'-methyl-4'-(4"-nitrophenyl)azo- 1'H-pyrazol-5'-ylazo)-3-methyl- H-pyrazol-5-on in the mediums containing glucose and different concentrations of degrade dye in batch systems. This dye was synthetized at Pamukkale Universtiy of Organic Chemistry research laboratory. Samples were collected on 10 days, and was detected by Shimadzu UV-1600A spectrophotometry. Decolorization study showed that this disazo dye was removed by more than 70% in 10 days. Laccase enzyme activity was detected in samples and then last sample was analyzed by GC-MS. Metabolites weren't showed in GC-MS result. It was concluded that T. versicolor could achieve the biodegradation of this new disazo dye.

  17. Invasive pulmonary aspergillosis caused by Aspergillus versicolor in a patient on mechanical ventilation

    OpenAIRE

    MV Pravin Charles; M Ravishankar; Easow, Joshy M; Noyal Mariya Joseph

    2011-01-01

    Aspergillus spp. often colonise the respiratory tract of critically ill patients in intensive care units and subsequently cause invasive disease. The risk of developing invasive disease is more in immunocompromised patients. Here we report a case of fatal invasive pulmonary aspergillosis caused by Aspergillus versicolor in a post-operative patient on mechanical ventilation, who did not respond to intravenous itraconazole. We then discuss the challenges involved in the accurate diagnosis of th...

  18. Novel Chromone Derivatives from Marine Fungus Aspergillus versicolor Isolated from the Sponge Xestospongia exigua

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    From the marine sponge Xestospongia exigua, fungal isolates of Aspergillus versicolor (Vuill)Triab were obtained. Isolation and purification of ethyl acetate extracts from culture filtrates of the fungus led to yield six new chromone derivatives namely aspergione A, aspergione B, aspergione C, aspergione D, aspergione E, aspergione F. The structures of all the new compounds were established on the basis of extensive spectroscopy (UV, MS, 1H and 13C NMR, COSY, HMQC and HMBC) analysis.

  19. Treatment of a Textile Effluent from Dyeing with Cochineal Extracts Using Trametes versicolor Fungus

    OpenAIRE

    Gabriela Arroyo-Figueroa; Graciela M. L. Ruiz-Aguilar; Leticia López-Martínez; Guillermo González-Sánchez; Germán Cuevas-Rodríguez; Refugio Rodríguez-Vázquez

    2011-01-01

    Trametes versicolor (Tv) fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. However, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. Experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of Tv fungus were done. Treatments to determine decolorization in t...

  20. Degradation of selected agrochemicals by the white rot fungus Trametes versicolor

    OpenAIRE

    Mir-Tutusaus, Josep Anton; Caminal i Saperas, Glòria; Sarrà i Adroguer, Montserrat

    2014-01-01

    Use of agrochemicals is a worldwide practice that exerts an important effect on the environment; therefore the search of approaches for the elimination of such pollutants should be encouraged. The degradation of the insecticides imiprothrin (IP) and cypermethrin (CP), the insecticide/nematicide carbofuran (CBF) and the antibiotic of agricultural use oxytetracycline (OTC) were assayed with the white rot fungus Trametes versicolor. Experiments with fungal pellets demonstrated extensive degradat...

  1. Four new spiroaxane sesquiterpenes and one new rosenonolactone derivative from cultures of Basidiomycete Trametes versicolor.

    Science.gov (United States)

    Wang, Su-Rui; Zhang, Ling; Chen, He-Ping; Li, Zheng-Hui; Dong, Ze-Jun; Wei, Kun; Liu, Ji-Kai

    2015-09-01

    Four new spiroaxane sesquiterpenes, tramspiroins A-D (1-4), one new rosenonolactone 15,16-acetonide (5), and the known drimane sesquiterpenes isodrimenediol (6) and funatrol D (7) have been isolated from the cultures of Basidiomycete Trametes versicolor. The structures of new compounds were elucidated by means of spectroscopic methods. Compounds 1-7 were investigated for their cytotoxicities against five human cancer cell lines. PMID:26136058

  2. Prevalence of different Malassezia species in pityriasis versicolor in central India

    Directory of Open Access Journals (Sweden)

    Chaudhary Rahul

    2010-01-01

    Full Text Available Background: In the last 10 years, different studies have shown interesting geographical variations in the prevalence of different Malassezia species in pityriasis versicolor. Aim: Identification of Malassezia species isolated from patients with pityriasis versicolor. Methods: In 100 patients with pityriasis versicolor, Malassezia species were identified by culture in Sabouraud′s dextrose agar containing cycloheximide with olive oil overlay and modified Dixon agar and by doing biochemical tests (catalase reaction, assimilation of glycine, and Tween utilisation tests. Results: In 10 patients, 10% KOH smear was negative, while in 90 patients the smear showed characteristic "spaghetti and meatball" appearance. Of these 90 cases, growth was obtained on modified Dixon′s agar in 87 cases. Fifty of the isolates (57.5% were M. globosa, 15 (17.2% were M. sympodialis, seven (8.0% were suspected M. sympodialis, 6 (6.9% each of the isolates were M. furfur and M. obtusa, and three (3.4% isolates were M. restricta. Conclusion: M. globosa was the most common species, followed by M. sympodialis, M. furfur, M. obtusa, and M. restricta.

  3. Amaranth decoloration by Trametes versicolor in a rotating biological contacting reactor.

    Science.gov (United States)

    Ramsay, Juliana; Shin, Maria; Wong, Sunny; Goode, Christopher

    2006-09-01

    Sequential batch and continuous operation of a rotating biological contacting (RBC) reactor and the effects of dissolved oxygen on the decoloration of amaranth by Trametes versicolor were evaluated. Amaranth belongs to the group of azo dyes which are potential carcinogens and/or mutagens that can be transformed into toxic aryl amines under anaerobic conditions. Cultivation of T. versicolor in a stirred tank reactor was found to be unsuitable for amaranth decoloration due to significant biomass fouling and increase in medium viscosity. Assuming that decoloration follows first-order kinetics, amaranth was decolorized more rapidly when T. versicolor was immobilized on jute twine in a RBC reactor operated either in a sequential batch (k=0.25 h(-1)) or in a continuous (0.051 h(-1)) mode compared to a stirred tank reactor (0.015 h(-1)). Oxygen was found to be essential for decoloration with the highest decoloration rates occurring at oxygen saturation. Although longer retention times resulted in more decoloration when the RBC was operated in the continuous mode (about 33% amaranth decoloration), sequential batch operation gave better results (>95%) under similar nutrient conditions. Our data indicate that the fastest decoloration should occur in the RBC using nitrogen-free Kirk's medium with 1 g/l glucose in sequential batch operation at rotational speeds and/or aeration rates which maintain oxygen saturation in the liquid phase.

  4. Enhancing the Antioxidant Ability of Trametes versicolor Polysaccharopeptides by an Enzymatic Hydrolysis Process.

    Science.gov (United States)

    Jhan, Mei-Hsin; Yeh, Ching-Hua; Tsai, Chia-Chun; Kao, Ching-Tian; Chang, Chao-Kai; Hsieh, Chang-Wei

    2016-01-01

    Polysaccharopeptides (PSPs) are among the main bioactive constituents of Trametes versicolor (T. versicolor). The purpose of this research was to investigate the antioxidant activities of enzymatic hydrolysates obtained from T. versicolor polysaccharopeptides by 80 U/mL β-1,3-glucanase (PSPs-EH80). The half-inhibitory concentration (IC50) of PSPs-EH80 in metal chelating assay, ABTS and DPPH radical scavenging test results were 0.83 mg/mL, 0.14 mg/mL and 0.52 mg/mL, respectively, which were lower than that of PSPs-EH 20 U/mL. The molecular weights of the PSPs-EH80 hydrolysates were 300, 190, 140 and 50 kDa, respectively, and the hydrolysis of polysaccharides by β-1,3-glucanase did not change the original functional group. PSPs-EH80 reduced the reactive oxygen species (ROS) content at least twice that of treatment without PSPs-EH80. In addition, an oxidative damage test showed that PSPs-EH80 can improve HaCaT cell survival. According to our results, PSP demonstrates the potential of anti-oxidative damage; besides, enzyme hydrolysis can improve the ability of the PSP. PMID:27626400

  5. Factors influencing offspring traits in the oviparous multi-clutched lizard, Calotes versicolor (Agamidae)

    Indian Academy of Sciences (India)

    Rajkumar S Radder; Bhagyashri A Shanbhag

    2004-03-01

    The determinants of offspring size and number in the tropical oviparous multi-clutched lizard, Calotes versicolor, were examined using both univariate and multivariate (path) analyses. In C. versicolor maternal snout-vent length (SVL) and body condition influence clutch mass and clutch size but have no significant influence on offspring size. The positive effect of maternal SVL and body condition on offspring number is counterbalanced by a negative effect of breeding time on egg mass. In fact, breeding time directly influences the offspring body mass and condition through variation in the egg mass. There is a trade-off between offspring mass and condition with offspring number, and breeding time influences both. Offspring hatched from the eggs of early (May–June) or mid (July–August) breeding periods invariably show lower mass and condition than those hatched from the eggs of late breeding season (September–October). Yet, there is no variation in offspring SVL among early, mid and late clutches. Thus, in C. versicolor offspring SVL is optimized while body mass and condition are not optimized.

  6. Malassezia yeast species isolated from Iranian patients with pityriasis versicolor in a prospective study.

    Science.gov (United States)

    Rasi, Abbas; Naderi, Reza; Behzadi, Ashkan Heshmatzade; Falahati, Mehraban; Farehyar, Shirin; Honarbakhsh, Yasamin; Akasheh, Amir Poya

    2010-07-01

    The goal of this study was to determine the prevalence of Malassezia species in pityriasis versicolor lesions and to examine if the range of species varies with patients characteristics such as: age, sex and family history and also clinical findings such as site and number of the lesions. In a prospective study from July 2006 to July 2007, the patients with a clinical diagnosis of pityriasis versicolor (n = 166) were asked to participate in the study. A total of 116 patients had positive culture for Malassezia species: M. globosa was found in 52 (31.3%) cases, M. furfur in 34 (20.5%) cases, M. pachydermatis in 12 (7.2%) cases, M. restricta in 12 (7.2%) cases, M. slooffiae in 6 (3.6%) cases. According to our data, M. globosa is the main species causing pityriasis versicolor, M. furfur was found to be the second-most frequent species. M. sympodialis and M. obtusa were not found in any case, and in 30.2% of patient's Malassezia culture was negative.

  7. Molecular epidemiology of Malassezia globosa and Malassezia restricta in Sudanese patients with pityriasis versicolor.

    Science.gov (United States)

    Saad, M; Sugita, T; Saeed, H; Ahmed, A

    2013-02-01

    Pityriasis versicolor is a superficial infection of the stratum corneum caused by Malassezia yeasts. The cutaneous Malassezia globosa and Malassezia restricta in Sudanese patients with pityriasis versicolor were elucidated using a molecular-based, culture-independent method and compared with that in healthy individuals. Scale samples were collected by applying an Opsite™ transparent dressing to lesional and non-lesional sites on 29 Sudanese patients with pityriasis versicolor and 54 healthy individuals. Malassezia DNA was extracted directly from the samples. The overall level of colonization by Malassezia globosa and Malassezia restricta was analyzed by real-time PCR using a TaqMan probe. The overall level of colonization by Malassezia at the lesional sites was higher than that at the non-lesional sites for all body sites, including the face, neck, cheeks, and trunk (2.7- to 6.0-fold increase). Both M. globosa and M. restricta were detected in patients and healthy individuals. However, M. globosa predominated at lesional sites, whereas the level of colonization by both species was similar in healthy individuals.

  8. Comparison between fluconazole and ketoconazole effectivity in the treatment of pityriasis versicolor.

    Science.gov (United States)

    Yazdanpanah, Mohammad Javad; Azizi, Hoda; Suizi, Behnaz

    2007-07-01

    Topical drugs are often effective in limited lesions of pityriasis versicolor; but in extensive cases, systemic drugs are more suitable. Previous studies have shown that ketoconazole and fluconazole are effective in 42-97% and 74-100% of lesions respectively. Our purpose was to compare the effectiveness of a single dose of 400 mg ketoconazole with two doses of 300 mg of fluconazole with 2 weeks interval. Ninety patients with extensive pityriasis versicolor (body involved area > or =25%) were randomly assigned to treatment with either a single dose of 400 mg of ketoconazole or with two doses of 300 mg of fluconazole with 2 weeks interval. One month after the treatment, the improvement rate and side effects were evaluated by clinical examination and questionnaire. Sixty cases (66.7%) completed the study. They were 51 males and nine females, with a mean age of 30 years. At the follow-up visit (1 month after the end of treatment), the improvement rate for ketoconazole (87.9%) was not significantly different from fluconazole (81.5%), (Fisher test: P = 0.37). Due to the hepatotoxicity of ketoconazole, fluconazole appears to be more suitable in the treatment of extensive pityriasis versicolor.

  9. Comparative Efficacy of 10% Sodium Sulfacetamide Lotion and 1% Clotrimazole Lotion in Treatment of Tinea versicolor

    Directory of Open Access Journals (Sweden)

    F Rad

    2008-01-01

    Full Text Available ABSTRACT: Introduction & Objective: Tinea versicolor is a common superficial fungal infection caused by normal skin flora and many topical and oral anti-fungal medications have been used for its treatment. The aim of this study was to compare the therapeutic effects of 10% sodium sulfacetamide lotion with 1% clotrimazole lotion in treatment of tinea versicolor. Materials & Methods: In this single-blind clinical trial 67 patients were divided into two groups using block randomization method. The first group consisted of 33 patients who were treated with 1% clotrimazole lotion. The second group included 34 patients who received 10% sodium sulfacetamide lotion. After 2 weeks of treatment, both groups were evaluated for clinical and paraclinical responses. The collected data were recorded in a data sheet and analyzed by X2 and independent T tests, using SPSS software. Results: After 2 weeks of treatment, clinical and paraclinical responses were seen in 22 patients (66.7% and 28 patients (84.8% in the first group (clotrimazole lotion and in 7 (20.6% and 8 (23.5% patients of the second group (sodium sulfacetamide lotion, respectively. Statistical analysis showed that the differences are significant. Conclusion: The therapeutic effect of 10% sodium sulfacetamide lotion is much less than that of 1% clotrimazole lotion in treatment of tinea versicolor.

  10. 云芝多糖研究进展%Review on Polysaccharide from Coriolus versicolor

    Institute of Scientific and Technical Information of China (English)

    王菲菲; 郝利民; 贾士儒; 陈强; 牛爽

    2012-01-01

    In recent years,edible and medicinal fungi are paid more and more attention for their various biological functions and thus become a research focus.Polysaccharide of Coriolus versicolor is isolated from Coriolus versicolor or its broth,and took more interesting from the industries of medicine,food,cosmetics and even chemistry and so on because of its superior biological activity and functions.This is a review on the polysaccharide structure,properties,production,usage,market of polysaccharide from Coriolus versicolor.It's also a comprehensive survey and reference of polysaccharide for further research and development.%近年来,药食用真菌因具有多种生物学功能而受到重视,成为研究热点。云芝多糖由云芝或从其发酵液中分离提取,具有突出的药食用功效和生物活性。文中从云芝多糖的结构、性质、提取纯化、发酵生产、用途与展望等方面进行了综述,为云芝多糖的进一步研究与开发提供参考。

  11. Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tehran, Iran

    Directory of Open Access Journals (Sweden)

    Zeraati Hojjat

    2004-05-01

    Full Text Available Abstract Background Pityriasis versicolor is a superficial infection of the stratum corneum which caused by a group of yeasts formerly named pityrosporium. The taxonomy of these lipophilic yeasts has recently been modified and includes seven species referred as Malassezia. The aim of this study is to compare the distribution of Malassezia species isolated from pityriasis versicolor lesions and those isolated from healthy skins. Methods Differentiation of all malassezia species performed using morphological features and physiological test including catalase reaction, Tween assimilation test and splitting of esculin. Results In pityriasis versicolor lesions, the most frequently isolated species was M. globosa (53.3%, followed by M. furfur (25.3%, M. sympodialis(9.3%, M. obtusa (8.1% and M. slooffiae (4.0%. The most frequently isolated species in the skin of healthy individuals were M. globosa, M. sympodialis, M. furfur, M. sloofiae and M. restricta which respectively made up 41.7%, 25.0%, 23.3%, 6.7% and 3.3% of the isolated species. Conclusions According to our data, M. globosa was the most prevalent species in the skin of healthy individuals which recovered only in the yeast form. However, the Mycelial form of M. globosa was isolated as the dominant species from pityriasis versicolor lesions. Therefore, the role of predisposing factors in the conversion of this yeast to mycelium and its subsequent involvement in pityriasis versicolor pathogenicity should be considered.

  12. Bioaugmentation of sewage sludge with Trametes versicolor in solid-phase biopiles produces degradation of pharmaceuticals and affects microbial communities.

    Science.gov (United States)

    Rodríguez-Rodríguez, Carlos E; Jelić, Aleksandra; Pereira, M Alcina; Sousa, Diana Z; Petrović, Mira; Alves, M Madalena; Barceló, Damià; Caminal, Glòria; Vicent, Teresa

    2012-11-01

    The use of sludge (biosolids) in land application may contribute to the spread of organic micropollutants as wastewater treatments do not completely remove these compounds. Therefore, the development of alternative strategies for sludge treatment is a matter of recent concern. The elimination of pharmaceuticals at pre-existent concentrations from sewage sludge was assessed, for the first time, in nonsterile biopiles by means of fungal bioaugmentation with Trametes versicolor (BTV-systems) and compared with the effect of autochthonous microbiota (NB-systems). The competition between the autochthonous fungal/bacterial communities and T. versicolor was studied using denaturing gradient gel electrophoresis (DGGE) and the cloning/sequencing approach. An inhibitory effect exerted by T. versicolor over bacterial populations was suggested. However, after 21 days, T. versicolor was no longer the main taxon in the fungal communities. The elimination profiles revealed an enhanced removal of atorvastatin-diclofenac-hydrochlorothiazide (during the whole treatment) and ranitidine-fenofibrate (at short periods) in the BTV biopiles in respect to NB biopiles, coincident with the presence of the fungus. For ibuprofen-clarithromycin-furosemide, the elimination profiles were similar irrespective of the system, and with carbamazepine no significant degradation was obtained. The results suggest that a fungal treatment with T. versicolor could be a promising process for the remediation of some pharmaceuticals in complex matrices such as biosolids. PMID:23030544

  13. Coleoptera Associated with Decaying Wood in a Tropical Deciduous Forest.

    Science.gov (United States)

    Muñoz-López, N Z; Andrés-Hernández, A R; Carrillo-Ruiz, H; Rivas-Arancibia, S P

    2016-08-01

    Coleoptera is the largest and diverse group of organisms, but few studies are dedicated to determine the diversity and feeding guilds of saproxylic Coleoptera. We demonstrate the diversity, abundance, feeding guilds, and succession process of Coleoptera associated with decaying wood in a tropical deciduous forest in the Mixteca Poblana, Mexico. Decaying wood was sampled and classified into four stages of decay, and the associated Coleoptera. The wood was identified according to their anatomy. Diversity was estimated using the Simpson index, while abundance was estimated using a Kruskal-Wallis test; the association of Coleoptera with wood species and decay was assessed using canonical correspondence analysis. Decay wood stage I is the most abundant (51%), followed by stage III (21%). We collected 93 Coleoptera belonging to 14 families, 41 genera, and 44 species. The family Cerambycidae was the most abundant, with 29% of individuals, followed by Tenebrionidae with 27% and Carabidae with 13%. We recognized six feeding guilds. The greatest diversity of Coleoptera was recorded in decaying Acacia farnesiana and Bursera linanoe. Kruskal-Wallis analysis indicated that the abundance of Coleoptera varied according to the species and stage of decay of the wood. The canonical analysis showed that the species and stage of decay of wood determined the composition and community structure of Coleoptera. PMID:26911160

  14. Coleoptera Associated with Decaying Wood in a Tropical Deciduous Forest.

    Science.gov (United States)

    Muñoz-López, N Z; Andrés-Hernández, A R; Carrillo-Ruiz, H; Rivas-Arancibia, S P

    2016-08-01

    Coleoptera is the largest and diverse group of organisms, but few studies are dedicated to determine the diversity and feeding guilds of saproxylic Coleoptera. We demonstrate the diversity, abundance, feeding guilds, and succession process of Coleoptera associated with decaying wood in a tropical deciduous forest in the Mixteca Poblana, Mexico. Decaying wood was sampled and classified into four stages of decay, and the associated Coleoptera. The wood was identified according to their anatomy. Diversity was estimated using the Simpson index, while abundance was estimated using a Kruskal-Wallis test; the association of Coleoptera with wood species and decay was assessed using canonical correspondence analysis. Decay wood stage I is the most abundant (51%), followed by stage III (21%). We collected 93 Coleoptera belonging to 14 families, 41 genera, and 44 species. The family Cerambycidae was the most abundant, with 29% of individuals, followed by Tenebrionidae with 27% and Carabidae with 13%. We recognized six feeding guilds. The greatest diversity of Coleoptera was recorded in decaying Acacia farnesiana and Bursera linanoe. Kruskal-Wallis analysis indicated that the abundance of Coleoptera varied according to the species and stage of decay of the wood. The canonical analysis showed that the species and stage of decay of wood determined the composition and community structure of Coleoptera.

  15. Carbon and nitrogen balance of leaf-eating sesarmid crabs ( Neoepisesarma versicolor) offered different food sources

    Science.gov (United States)

    Thongtham, Nalinee; Kristensen, Erik

    2005-10-01

    Carbon and nitrogen budgets for the leaf-eating crab, Neoepisesarma versicolor, were established for individuals living on pure leaf diets. Crabs were fed fresh (green), senescent (yellow) and partly degraded (brown) leaves of the mangrove tree Rhizophora apiculata. Ingestion, egestion and metabolic loss of carbon and nitrogen were determined from laboratory experiments. In addition, bacterial abundance in various compartments of the crabs' digestive tract was enumerated after dissection of live individuals. Ingestion and egestion rates (in terms of dry weight) were highest, while the assimilation efficiency was poorest for crabs fed on brown leaves. The low assimilation efficiency was more than counteracted by the high ingestion rate providing more carbon for growth than for crabs fed green and yellow leaves. In any case, the results show that all types of leaves can provide adequate carbon while nitrogen was insufficient to support both maintenance (yellow leaves) and growth (green, yellow and brown leaves). Leaf-eating crabs must therefore obtain supplementary nitrogen by other means in order to meet their nitrogen requirement. Three hypotheses were evaluated: (1) crabs supplement their diet with bacteria and benthic microalgae by ingesting own faeces and/or selective grazing at the sediment surface; (2) assimilation of symbiotic nitrogen-fixing bacteria in the crabs' own intestinal system; and (3) nitrogen storage following occasional feeding on animal tissues (e.g. meiofauna and carcasses). It appears that hypothesis 1 is of limited importance for N. versicolor since faeces and sediment can only supply a minor fraction of the missing nitrogen due to physical constraints on the amount of material the crabs can consume. Hypothesis 2 can be ruled out because tests showed no nitrogen fixation activity in the intestinal system of N. versicolor. It is therefore likely that leaf-eating crabs provide most of their nitrogen requirement from intracellular deposits

  16. Decoloration of Amaranth by the white-rot fungus Trametes versicolor. Part II. Verification study.

    Science.gov (United States)

    Gavril, Mihaela; Hodson, Peter V

    2007-02-01

    The involvement of lignin peroxidase (LiP) in the decoloration of the mono-azo substituted napthalenic dye Amaranth was investigated with pure enzymes and whole cultures of Trametes versicolor. The verification study confirmed that LiP has a direct influence on the initial decoloration rate and showed that another enzyme, which does not need hydrogen peroxide to function and is not a laccase, also plays a role during decoloration. These results confirm the results of a previous statistical analysis. Furthermore, the fungal mycelium affects the performance of the decoloration process.

  17. Bioindication Potential of the Coleoptera

    Directory of Open Access Journals (Sweden)

    Belitskaya Mariya Nikolaevna

    2015-09-01

    Full Text Available Different families of Coleoptera ambiguously respond to the pollution of SPZs with industrial emissions. For example, the SPZ of Volgograd aluminum plant has the changing biodiversity of insect communities at different distances from the pollution source. The increasing level of pollution is accompanied by the reduction in species abundance. At a distance of 200 m a special ecological zone with the specific composition of the entomofauna was formed. It is significantly different from other habitats. No Cerambycidae species may survive in the zone of maximum pollution, and the number of Curculionidae species is reduced significantly. The number of Cerambycidae decreases by more than 40 % in the presence of even minimal contamination. The most sensitive bioindicators are represented by such insects as Cerambycidae, Curculionidae and Chrysomelidae. Changes in the indices can be described by the function y = arctan (x, where x is the distance from the pollution source (in meters. The specificity of this function is to identify levels of possible changes of species richness and numerical abundance of communities. On the basis of trigonometric functions describing the changes in the species composition and abundance, the authors offered the method for assessing the quality of the environment in SPZs. The use of three families of insects opens up prospects of differentiation zones of technogenic pressure.

  18. Dectin-1 and IL-17A suppress murine asthma induced by Aspergillus versicolor but not Cladosporium cladosporioides due to differences in β-glucan surface exposure.

    Science.gov (United States)

    Mintz-Cole, Rachael A; Gibson, Aaron M; Bass, Stacey A; Budelsky, Alison L; Reponen, Tiina; Hershey, Gurjit K Khurana

    2012-10-01

    There is considerable evidence supporting a role for mold exposure in the pathogenesis and expression of childhood asthma. Aspergillus versicolor and Cladosporium cladosporioides are common molds that have been implicated in asthma. In a model of mold-induced asthma, mice were repeatedly exposed to either A. versicolor or C. cladosporioides spores. The two molds induced distinct phenotypes, and this effect was observed in both BALB/c and C57BL/6 strains. C. cladosporioides induced robust airway hyperresponsiveness (AHR), eosinophilia, and a predominately Th2 response, whereas A. versicolor induced a strong Th17 response and neutrophilic inflammation, but very mild AHR. Neutralization of IL-17A resulted in strong AHR and eosinophilic inflammation following A. versicolor exposure. In Dectin-1-deficient mice, A. versicolor exposure resulted in markedly attenuated IL-17A and robust AHR compared with wild-type mice. In contrast, C. cladosporioides induced AHR and eosinophilic inflammation independent of IL-17A and Dectin-1. A. versicolor, but not C. cladosporioides, spores had increased exposure of β-glucans on their surface and were able to bind Dectin-1. Thus, the host response to C. cladosporioides was IL-17A- and Dectin-1-independent, whereas Dectin-1- and IL-17A-dependent pathways were protective against the development of asthma after exposure to A. versicolor.

  19. Evaluation of support materials for the surface immobilization and decoloration of amaranth by Trametes versicolor.

    Science.gov (United States)

    Shin, M; Nguyen, T; Ramsay, J

    2002-10-01

    The ability of Trametes versicolor ATCC 20869 to colonize several natural and synthetic materials (wheat straw, jute, hemp, maple woodchips, and nylon and polyethylene teraphthalate fibers) and to subsequently decolorize amaranth was evaluated. Jute was found to be the best support material as T. versicolor grew well on it without color leaching from the support and without loss of the jute's integrity over a 4 week period. The fungus immobilized on jute, straw and hemp decolorized amaranth (50 mg l(-1)) at a rate of about 5 mg l(-1) h(-1) without glucose being added. When 1 g l(-1) glucose was added, the dye was degraded more quickly (about 8 mg l(-1) h(-1)). Decoloration did not occur in a suspension culture without glucose. As the number of decoloration cycles increased, the rate of decoloration decreased. This rate was restored to its original level after the biomass was incubated in fresh growth medium for 5 days. With all immobilization supports, the toxicity of the medium before and after decoloration was the same or lower.

  20. Function of the iron-binding chelator produced by Coriolus versicolor in lignin biodegradation

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    An ultrafiltered low-molecular-weight preparation of chelating compounds was isolated from a wood-containing culture of the white-rot basidiomycete Coriolus versicolor. This preparation could chelate Fe3+ and reduce Fe3+ to Fe2+, demonstrating that the substance may serve as a ferric chelator, oxygen-reducing agent, and redox-cycling molecule, which would include functioning as the electron transport carrier in Fenton reaction. Lignin was treated with the iron-binding chelator and the changes in structure were investigated by 1H-NMR, 13C-NMR, difference spectrum caused by ionization under alkaline conditions and nitrobenzene oxidation. The results indicated that the iron-binding chelator could destroy the β-O-4 bonds in etherified lignin units and insert phenolic hydroxyl groups. The low-molecular-weight chelator secreted by C. versicolor resulted in new phenolic substructures in the lignin polymer, making it susceptible to attack by laccase or manganese peroxidase. Thus, the synergic action of the iron-binding chelator and the lignocellulolytic enzymes made the substrate more acces- sible to degradation.

  1. Function of the iron-binding chelator produced by Coriolus versicolor in lignin biodegradation

    Institute of Scientific and Technical Information of China (English)

    WANG Lu; YAN WenChao; CHEN JiaChuan; HUANG Feng; GAO PeiJi

    2008-01-01

    An ultrafiltered low-molecular-weight preparation of chelating compounds was isolated from a wood-containing culture of the white-rot basidiomycete Coriolus versicolor. This preparation could chelate Fe3+ and reduce Fe3+ to Fe2+, demonstrating that the substance may serve as a ferric chelator,oxygen-reducing agent, and redox-cycling molecule, which would include functioning as the electron transport carrier in Fenton reaction. Lignin was treated with the iron-binding chelator and the changes in structure were investigated by 1H-NMR, 13C-NMR, difference spectrum caused by ionization under alkaline conditions and nitrobenzene oxidation. The results indicated that the iron-binding chelator could destroy the β-O-4 bonds in etherified lignin units and insert phenolic hydroxyl groups. The low-molecular-weight chelator secreted by C. versicolor resulted in new phenolic substructures in the lignin polymer, making it susceptible to attack by laccase or manganese peroxidase. Thus, the synergic action of the iron-binding chelator and the lignocellulolytic enzymes made the substrate more accessible to degradation.

  2. Distribution of Malassezia species in patients with pityriasis versicolor in Northern Iran

    Directory of Open Access Journals (Sweden)

    Shokohi T

    2009-01-01

    Full Text Available Purpose : Malassezia yeasts are globally distributed agents of pityriasis versicolor and are implicated in the pathogenesis of seborrhoeic and atopic dermatitis. The aim of this study is to identify the Malassezia species obtained from pityriasis versicolor patients, using morphological, biochemical, physiological as well as Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP methods. Materials and Methods: The identification of Malassezia species is performed according to microscopic features and physiological characteristics, including catalase reaction and Tween assimilation tests. The DNA is extracted from cultured Malassezia using the glass bead, phenol-chloroform method. The internal transcribed spacer 1(ITS1 region is amplified and there is restricted digestion of the PCR products with two enzymes Cfo I and Bst F5I. Results : The most commonly isolated species is M. globosa (47.6%. RFLP analysis of the PCR products of the ITS1 region is in complete agreement with those from the DNA sequences of the internal transcribed spacer (ITS 1 region and the biochemical tests. Conclusion : Based on the findings of this study, it can be concluded that PCR-RFLP is a relatively simple and quick method, completely comparable to the routine methods used for Malassezia identification.

  3. Expression of antimicrobial peptides and toll-like receptors is increased in tinea and pityriasis versicolor.

    Science.gov (United States)

    Brasch, J; Mörig, A; Neumann, B; Proksch, E

    2014-03-01

    In superficial tinea and pityriasis versicolor, the causative fungi are for the most part confined to the stratum corneum which is barely reached by leukocytes. Therefore, a role of non-cellular components in the epidermal antifungal defence was suggested. To investigate the presence of such factors in these infections, the expression of human beta defensins 2 and 3 (hBD-2, hBD-3), RNase 7, psoriasin, toll-like receptors 2, 4 and 9 (TLR2, TLR4 and TLR9) and dectin 2 was analysed by use of immunostainings in skin biopsies. We found that hBD2, hBD3, psoriasin, RNase7, TLR2 and TLR4 were significantly more often expressed in distinct layers of lesional epidermis as compared with uninfected epidermis. In both infections but not in normal skin, hBD2 and hBD3 were commonly expressed within the stratum corneum and in the stratum granulosum. Similarly, psoriasin was seen more often in the upper skin layers of both infections as compared with normal skin. No significant differences between normal and infected skin were found for the expression of TLR9 and dectin 2. Our findings clearly show the expression of specific antimicrobial proteins and defence-related ligands in superficial tinea as well as in pityriasis versicolor, suggesting that these factors contribute to fungal containment.

  4. Antifungal activity of oregano extract against A. Versicolor, E. Nidulans and Eurotium spp.: Producers of sterigmatocystin

    Directory of Open Access Journals (Sweden)

    Kocić-Tanackov Sunčica D.

    2011-01-01

    Full Text Available The paper presents the influence of oregano extract (Origanum vulgare L. on growth of Aspergillus versicolor, Emericella nidulans, Eurotium herbariorum, E. amstelodami, E. chevalieri and E. rubrum - producers of sterigmatocystin (STC isolated from salads. Antifungal tests were performed by agar plates method. The composition of the active component of extract was determined by GC-MS method and the major components were: carvacrol (34.20%, triacetin (22.91%, carvone (18.05%, p-cymene (8.05% and thymol (3.74%. The examined extract showed the ability to reduce mold growth at all applied concentrations. Minimum inhibitory concentrations (MIC for E. nidulans, E. chevalieri and E. amstelodami were 2.5% (v/v and over 2.5% (v/v for A. versicolor. At 1.5% (v/v concentration the extract completely inhibited the growth of E. rubrum, whereas higher dose of 2.5% (v/v was fungicidal against E. herbariorum. Besides its sensory role in food products, the examined oregano extract also exhibits antifungal activities against producers of STC.

  5. Hormonal modulation of phonotaxis and advertisement-call preferences in the gray treefrog (Hyla versicolor)

    Science.gov (United States)

    Gordon, Noah M.; Gerhardt, H. Carl

    2009-01-01

    Hormonal levels fluctuate during the breeding season in many anurans, but the identity of the hormones that modulate breeding behavior and their effects remain unclear. We tested the influence of a combined treatment of progesterone and prostaglandin on phonotaxis, the key proceptive reproductive behavior of female anurans. First, we found that female gray treefrogs (Hyla versicolor) treated with progesterone and prostaglandin exhibited phonotaxis to synthetic male advertisement signals significantly more often than animals treated with ringers vehicle or uninjected controls. Responsive females had greater levels of plasma progesterone and estradiol compared to both control groups, suggesting that these steroids may be promoting phonotaxis. Second, we found that the selectivity of hormonally-induced phonotaxis in H. versicolor was similar to that observed in freshly captured breeding animals. Females made the same choices between acoustic signals after hormone treatments in tests of frequency, call rate and pulse rate, compared to their responses without treatment immediately after collection from the breeding chorus. The preference for a longer call was, however, significantly weaker after hormone induction of phonotaxis. Hormonally primed females were also less likely to respond in any test and took longer to respond than did freshly collected females. Consequently, our study shows how progesterone-prostaglandin induced phonotaxis in female treefrogs influences both the quality and quantity of phonotaxis, relative to that exhibited by naturally breeding females. PMID:18926827

  6. Walking to survive. Searching, feeding and egg production of the carabid beetle Pterostichus coerulescens L. (= Poecilus versicolor Sturm).

    NARCIS (Netherlands)

    Mols, P.J.M.

    1993-01-01

    This study concerns the prey-searching and feeding behaviour of the polyphagous groundbeetle Pterostichus coerulescens L. ( = Poecilus versicolor Sturm), a common species on sandy soils. This ground beetle rarely flies, thus preysearching behaviour involves walking. The beetle is diurnal. As object

  7. The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis

    Science.gov (United States)

    Matijašević, Danka; Pantić, Milena; Rašković, Božidar; Pavlović, Vladimir; Duvnjak, Dunja; Sknepnek, Aleksandra; Nikšić, Miomir

    2016-01-01

    The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL-1. C. versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms. PMID:27540376

  8. [Missing granulocytic infiltrate in pityriasis versicolor--indication of specific anti-inflammatory activity of the pathogen?].

    Science.gov (United States)

    Wroblewski, N; Bär, Silja; Mayser, P

    2005-01-01

    The yeast Malassezia furfur is a part of the resident flora of human skin. It causes various diseases such as pityriasis versicolor, which hardly shows signs of inflammation despite marked clinical symptoms (e.g. hypopigmentation). The pathophysiology related morphological picture might give a clue to this phenomenon. As a part of the literature data are controversial, the present study compared the inflammatory infiltrate of pityriasis versicolor with that of tinea corporis in 40 human skin preparations each from diagnostic specimens. All preparations were stained with HE and PAS. Neutrophilic granulocytes were counted in the HE stain, and hyphae and spores in the PAS stain. The number of counted cells was related to the size of the respective area and the values were compared between pityriasis and tinea corporis. Significantly, more neutrophilic granulocytes were found with tinea corporis (P > 0.01), while they were virtually not demonstrable with pityriasis versicolor. It is surprising that fungal load in the stratum corneum is significantly higher with pityriasis versicolor (P > 0.01). Obviously the immune response involving neutrophilic granulocytes does not occur despite high bacterial load. This might be explained by reduced immunogenicity because of high content of lipids in the cell membrane. Furthermore, pityriarubins that are produced during tryptophan metabolism might be involved, which, in a stimulus-dependent manner, can suppress the ROS production of neutrophilic granulocytes in vivo.

  9. Humoral immunity to Malassezia furfur serovars A, B and C in patients with pityriasis versicolor, seborrheic dermatitis and controls.

    Science.gov (United States)

    Ashbee, H R; Fruin, A; Holland, K T; Cunliffe, W J; Ingham, E

    1994-10-01

    This study examined the humoral immune responses to Malassezia furfur serovars A, B and C of 10 patients with pityriasis versicolor, 10 patients with seborrheic dermatitis and 20 age- and sex-matched controls. A transferable solid-phase ELISA was used to determine titres of total Igs, IgM, IgA and IgG specific to M. furfur serovars A, B and C. The results demonstrated that patients with seborrheic dermatitis had a significantly higher titre of total Igs to serovar A than patients with pityriasis versicolor; and that patients with seborrheic dermatitis had a significantly higher titre of IgA to serovar C than patients with pityriasis versicolor. The titres of total Igs for controls and patients with seborrheic dermatitis were significantly lower to serovar B than to serovar C. A modified TSP ELISA was used to determine the titres of the IgG subclasses. Titres of IgG1,3,4 to serovar B were significantly higher in seborrheic dermatitis patients than pityriasis versicolor patients and titres of IgG3 to serovar A were significantly higher in seborrheic dermatitis patients than pityriasis versicolor patients. However, despite the differences between the patient groups, none of these results was significantly different to those of controls. Thus, this study did not demonstrate any differences in humoral immunity of patients suffering from Malassezia-associated dermatoses when compared to normal controls. These results may suggest that the humoral immune response to M. furfur is not related to the pathogenesis of Malassezia-associated dermatoses, but simply to the carriage of M. furfur on the skin.

  10. 广西产云芝的云芝多糖HPLC含量测定的方法%Content Determination Method with HPLC of Coriolus versicolor Polysaccharide in Versicolor Produced in Guangxi Province

    Institute of Scientific and Technical Information of China (English)

    陈建红; 黄岛平; 黄艳

    2012-01-01

    [Objective] The research aimed to determine the content of Coriolus versicolor polysaccharide in 35 copies of Coriolus versicolor in different regions and different times in Guangxi and 15 copies collected in different parts of China. [Method] The hydrolyzate of polysaccharide was determined with HPLC. According to the chromatographic peak of monosaccharide reference substance and samples, the composition of the monosaccharide in Coriolus versicolor polysaccharide was determined. [Result] Coriolus versicolor polysaccharide composes of mannose, rhamnose, galacturonic acid, glucose, galactose and xylose. The sterling Coriolus versicolor polysaccharide (more than 80% ) composes of glucose , rhamnose, galactose, mannose, galacturonic acid and xylose and their mole ratio is 5, 93: 2. 20: 0,90: 1. 26: 0, 81:0,43. [ Conclusion] The composition of Coriolus versicolor polysaccharide in different area and different collection time in Guangxi was same basically.%[目的]测定广西不同地区和不同时间采集云芝35份和全国不同地区采集云芝15份云芝中的多糖组成.[方法]对多糖水解物进行HPLC测定.根据单糖对照品、样品色谱峰,确定其单糖的组成.[结果]云芝多糖的单糖组成为甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖.纯品云芝多糖(80%以上)葡萄糖、鼠李糖、半乳糖、甘露糖、半乳糖醛酸、木糖的摩尔比为5.93∶2.20∶0.90∶ 1.26∶0.81∶0.43.[结论]广西不同地区和不同时间采集云芝多糖组成基本相同.

  11. Dectin-1 and IL-17A suppress murine asthma induced by Aspergillus versicolor but not Cladosporium cladosporioides due to differences in beta-glucan surface exposure1

    OpenAIRE

    Mintz-Cole, Rachael A.; Gibson, Aaron M.; Bass, Stacey A.; Budelsky, Alison L.; Reponen, Tiina; Hershey, Gurjit K. Khurana

    2012-01-01

    There is considerable evidence supporting a role for mold exposure in the pathogenesis and expression of childhood asthma. Aspergillus versicolor and Cladosporium cladosporioides are common molds that have been implicated in asthma. In a model of mold-induced asthma, mice were repeatedly exposed to either A. versicolor or C. cladosporioides spores. The two molds induced distinct phenotypes and this effect was observed in both Balb/c and C57BL/6 strains. C. cladosporioides induced robust airwa...

  12. Production of laccase by Coriolus versicolor and its application in decolorization of dyestuffs : (Ⅰ) Production of laccase by batch and repeated-batch processes

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The production of laccase by Coriolus versicolor wasstudied. The effect of cultivation conditions on laccase productionby Coriolus versicolor was examined to obtain optimal medium andcultivation conditions. Both batch and repeated-batch processeswere performed for laccase production. In repeated-batchfermentation with self-immobilized mycelia, total of 14 cycles wereperformed with laccase activity in the range between 3.4 and 14.8U/ml.

  13. Decoloration of Amaranth by the white-rot fungus Trametes versicolor. Part I. Statistical analysis.

    Science.gov (United States)

    Gavril, Mihaela; Hodson, Peter V; McLellan, Jim

    2007-02-01

    The white-rot fungus Trametes versicolor decolorized the mono-azo-substituted naphthalenic dye Amaranth. The relationship between the amount of enzymes present in the system and the efficiency of the decoloration process was investigated. The two responses used to quantify the process of decoloration (i.e., initial decoloration rate, v0, and the percent concentration of dye decolorized in 1 h, %c) were correlated with the amount of three enzymes considered for the study (lignin peroxidase, manganese peroxidase, and laccase) and analyzed through stepwise regression analysis (forward, backward, and mixed). The results of the correlation analysis and those of the regression analysis indicated that lignin peroxidase is the enzyme having the greatest influence on the two responses.

  14. Complete mitochondrial genome of the versicoloured emerald hummingbird Amazilia versicolor, a polymorphic species.

    Science.gov (United States)

    Prosdocimi, Francisco; Souto, Helena Magarinos; Ruschi, Piero Angeli; Furtado, Carolina; Jennings, W Bryan

    2016-09-01

    The genome of the versicoloured emerald hummingbird (Amazilia versicolor) was partially sequenced in one-sixth of an Illumina HiSeq lane. The mitochondrial genome was assembled using MIRA and MITObim software, yielding a circular molecule of 16,861 bp in length and deposited in GenBank under the accession number KF624601. The mitogenome contained 13 protein-coding genes, 22 transfer tRNAs, 2 ribosomal RNAs and 1 non-coding control region. The molecule was assembled using 21,927 sequencing reads of 100 bp each, resulting in ∼130 × coverage of uniformly distributed reads along the genome. This is the forth mitochondrial genome described for this highly diverse family of birds and may benefit further phylogenetic, phylogeographic, population genetic and species delimitation studies of hummingbirds. PMID:25758043

  15. Antimicrobial activity of crude methanolic extracts from Ganoderma lucidum and Trametes versicolor

    Directory of Open Access Journals (Sweden)

    Lukáš Hleba

    2014-11-01

    Full Text Available In this paper the antimicrobial activity of crude methanolic extracts obtained from Ganoderma lucidum and Trametes versicolor were investigated. The antimicrobial activities of the extracts against E. coli, P. aeruginosa, S. epidermis, E. raffinosus, S. cerevisiae and C. albicans were determined by the microbroth dillution method according by EUCAST in 96-well microplates. Microorganisms were obtained from Czech Collection of Microorganisms. Absorbances after and before the experiment were subtracted, converted to binary system and obtained values to Probit analysis were used. Of the two macromycetes extracts tested, not all extracts showed antimicrobial activity in tested MICs range. The highest antimicrobial activity showed the both extracts to Saccharomyces cerevisiae. The less antimicrobial effects had the both macromycetes extracts to Staphylococcus epidermis. Antimicrobial activity of macromycetes methanolic crude extracts to others tested microorganisms showed no effect or used concentration could be higher.

  16. Biological decolourisation of pulp mill effluent using white rot fungus Trametes versicolor.

    Science.gov (United States)

    Srinivasan, S V; Murthy, D V S; Swaminathan, T

    2012-07-01

    The conventional biological treatment methods employed in the pulp and paper industries are not effective in reducing the colour and chemical oxygen demand (COD). The white-rot fungi are reported to have the ability to biodegrade the lignin and its derivatives. This paper is focused on the biological treatment of pulp mill effluent from a bagasse-based pulp and paper industry using fungal treatment. Experiments were conducted using the white rot fungus, Trametes versicolor in shake flasks operated in batch mode with different carbon sources. The decolourisation efficiencies of 82.5% and 80.3% were obtained in the presence of 15 g/L and 5 g/L of glucose and sucrose concentrations respectively with a considerable COD reduction. The possibility of reusing the grown fungus was examined for repeated treatment studies.

  17. Comparative study of ketoconazole versus selenium sulphide shampoo in pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Aggarwal K

    2003-03-01

    Full Text Available Forty patients suffering from pityriasis versicolor were treated with either 2% ketoconazole shampoo (20 patients or 2.5% selenium sulphide shampoo (20 patients, once a week for three weeks. On global assessment after one month of start of therapy, 19 (95% out of 20 patients treated with ketoconazole shampoo were cured while one case had mild residual disease. In selenium sulphide shampoo group, 17 (85% out of 20 patients were cured, one had mild residual disease and two had considerable residual disease. No significant difference was observed in the response rates in the two groups. Relapse occurred in one patient of ketoconazole group and two patients of selenium sulphide group during the follow - up period of three months.

  18. Complete mitochondrial genome of the versicoloured emerald hummingbird Amazilia versicolor, a polymorphic species.

    Science.gov (United States)

    Prosdocimi, Francisco; Souto, Helena Magarinos; Ruschi, Piero Angeli; Furtado, Carolina; Jennings, W Bryan

    2016-09-01

    The genome of the versicoloured emerald hummingbird (Amazilia versicolor) was partially sequenced in one-sixth of an Illumina HiSeq lane. The mitochondrial genome was assembled using MIRA and MITObim software, yielding a circular molecule of 16,861 bp in length and deposited in GenBank under the accession number KF624601. The mitogenome contained 13 protein-coding genes, 22 transfer tRNAs, 2 ribosomal RNAs and 1 non-coding control region. The molecule was assembled using 21,927 sequencing reads of 100 bp each, resulting in ∼130 × coverage of uniformly distributed reads along the genome. This is the forth mitochondrial genome described for this highly diverse family of birds and may benefit further phylogenetic, phylogeographic, population genetic and species delimitation studies of hummingbirds.

  19. Chemical constituents of Coriolus versicolor%云芝化学成分研究

    Institute of Scientific and Technical Information of China (English)

    秦向东; 刘吉开

    2012-01-01

    从真菌云芝子实体中分离鉴定了6个化合物和1个昆合物,分别为:(22E,24R) -ergosta-7,22-dien - 3β -ol(1)、(22E,24R) - ergosta -6,22 - dien - 3β,5α,8 α- triol (2)、5α,6α-epoxy- (22E,24R) -ergosta -8,22 - dien - 3β,7α - diol (3)、二十四碳酸(4)、二十六碳酸(5)和α,α- trehalose(6);混合物可推测主要由russulamide (7)、ascolipid C(8)、ascolipid D(9)组成.除化合物1外,其余化合物均为该种首次分离得到.%Six compounds and a white amorphous powder were isolated from the fruiting bodies of Cori-olus versicolor by column chromatographic methods, structures established by spectroscopic methods. Six compounds were determined to be (22 E, 24 R) - ergosta - 7, 22 - dien -3β-ol (1), (22E, 24R) -ergosta-6, 22-dien-3β, 5α, 8α - triol (2) , 5α, 6α-epoxy- (22E, 24R) -ergosta -8, 22 -dien -3β, 7 α - diol (3) , tetracosanoic acid (4) , hexacosanoic acid (5) , and a, a - trehalose (6) , and the white powder to be a mixture of russulamide (7) , ascolipid C (8) , ascolip-id D (9 ). Compounds 2-9 were isolated for the first time from C. versicolor.

  20. Cresols utilization by Trametes versicolor and substrate interactions in the mixture with phenol.

    Science.gov (United States)

    Alexieva, Zlatka; Yemendzhiev, Hyusein; Zlateva, Plamena

    2010-07-01

    The ability of the white rot fungus Trametes versicolor strain 1 to degrade and utilize methylated phenols (cresols) was established for the first time in a medium not containing any other carbon components. The data obtained demonstrated the better potential of the strain to assimilate p-cresol instead of o- or m- cresol. The 0.5 g/l p-cresol provided was degraded in full after 96 h. The effect of a dual substrate mixture (0.3 g/l phenol + 0.2 g/l p-cresol) on the growth behavior and degradation capacity of the investigated strain was examined. The cell-free supernatants were analyzed by HPLC. It was established that the presence of p-cresol had not prevented complete phenol degradation but had a significant delaying effect on the phenol degradation dynamics. Phenol hydroxylase, catechol 1.2-dioxygenase and cis,cis-muconate cyclase activities were obtained in conditions of single and mixed substrates cultivation. The influence of different phenolic substrates on phenol hydroxylase activity in Trametes versicolor 1 was established. The mathematical models describing the dynamics of single substrates' utilization as well as the mutual influence of phenol and p-cresol in the mixture were developed on the bases of Haldane kinetics. The estimated interaction coefficients (I(ph/cr) = 4.72, I(cr/ph) = 7.46) demonstrated the significant inhibition of p-cresol on phenol biodegradation and comparatively low level of influence of phenol presence on the p-cresol degradation. Molecular 18S RNA gene taxonomy of the investigated strain was performed.

  1. Degradation of selected agrochemicals by the white rot fungus Trametes versicolor.

    Science.gov (United States)

    Mir-Tutusaus, Josep Anton; Masís-Mora, Mario; Corcellas, Cayo; Eljarrat, Ethel; Barceló, Damià; Sarrà, Montserrat; Caminal, Glòria; Vicent, Teresa; Rodríguez-Rodríguez, Carlos E

    2014-12-01

    Use of agrochemicals is a worldwide practice that exerts an important effect on the environment; therefore the search of approaches for the elimination of such pollutants should be encouraged. The degradation of the insecticides imiprothrin (IP) and cypermethrin (CP), the insecticide/nematicide carbofuran (CBF) and the antibiotic of agricultural use oxytetracycline (OTC) were assayed with the white rot fungus Trametes versicolor. Experiments with fungal pellets demonstrated extensive degradation of the four tested agrochemicals, at rates that followed the pattern IP>OTC>CP>CBF. In vitro assays with laccase-mediator systems showed that this extracellular enzyme participates in the transformation of IP but not in the cases of CBF and OTC. On the other hand, in vivo studies with inhibitors of cytochrome P450 revealed that this intracellular system plays an important role in the degradation of IP, OTC and CBF, but not for CP. The compounds 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (DCCA) and 3-phenoxybenzoic acid (PBA) were detected as transformation products of CP, as a result of the breakdown of the molecule. Meanwhile, 3-hydroxycarbofuran was detected as a transformation product of CBF; this metabolite tended to accumulate during the process, nonetheless, the toxicity of the system was effectively reduced. Simultaneous degradation of CBF and OTC showed a reduction in toxicity; similarly, when successive additions of OTC were done during the slower degradation of CBF, the fungal pellets were able to degrade both compounds. The simultaneous degradation of the four compounds successfully took place with minimal inhibition of fungal activity and resulted in the reduction of the global toxicity, thus supporting the potential use of T. versicolor for the treatment of diverse agrochemicals. PMID:25217998

  2. Phenylquinolinones with antitumor activity from the Indian Ocean-derived fungus Aspergillus versicolor Y31-2

    Science.gov (United States)

    Li, Peihai; Fan, Yaqin; Chen, Hao; Chao, Yaxi; Du, Ning; Chen, Junhui

    2016-09-01

    Two phenylquinolinones, including one new compound ( 1) and a previously isolated compound ( 2), were isolated from the ethyl acetate extracts of the fungus Aspergillus versicolor Y31-2, which was obtained from seawater samples collected from the Indian Ocean. The structures of these compounds were established by spectroscopic analyses. 4-(3-Hydroxyphenyl)-3-methoxyquinolin-2(1H)-one ( 1) exhibited moderate cytotoxicity against MCF-7 (human breast carcinoma cell line) and SMMC-7721 (human liver cancer cell line) cells with IC50 values of 16.6 and 18.2 μmol/L, respectively. To the best of our knowledge, this study represents the first reported account of the isolation of compounds 1 and 2 as the secondary metabolites of the seawater derived fungus Aspergillus versicolor from the Indian Ocean.

  3. Ensaio terapêutico com o Ketoconazol na pitiríase versicolorTherapeutic trial with Ketoconazole in PV

    Directory of Open Access Journals (Sweden)

    Leila Dagher

    1982-11-01

    Full Text Available Experimentação terapêutico-laboratorial com o Ketoconazol na Pitiríase Versicolor, obtendo os seguintes resultados: - Curados, 16 (88,88% pacientes; Melhorados, 2 pacientes (11,11%, Inalterados e Piorados, nenhum caso (0,00%, no total de 18 doentes estudados. Do ponto de vista laboratorial, houve negativação dos exames micológicos em 100,00% dos casos.Eighteen patients with pityriasis versicolor were treated with a single daily dose of 200mg of Ketoconazole administered with a meal, for a period of 30 days. Evaluation was based on clinical and mycological responses. Clinical and parasitoligical cure was obtained in 16 (88,88% patients and marked clinical improvement in 2(11,11%. No undesirable side effects were observed in the treatment.

  4. ``Winter'' aggregations, colony cycle, and seasonal phenotypic change in the paper wasp Polistes versicolor in subtropical Brazil

    Science.gov (United States)

    Gobbi, Nivar; Noll, Fernando B.; Penna, Marcelo A. H.

    2006-10-01

    Social wasps from temperate zones have clear annual colony cycles, and the young queens hibernate during winter. In the subtropics, the only previously reported evidence for the existence of “hibernation” is the facultative winter aggregations of females during harsh climate conditions. As in temperate-zone species analyzed so far, we show in this study that in the paper wasp, Polistes versicolor, a subtropical species, body size increases as an unfavorable season approaches. Our morphological studies indicate that larger females come from winter aggregations—that is, they are new queens. Multivariate analyses indicate that size is the only variable analyzed that shows a relationship to the differences. Given the absence of a harsh climate, we suggest that the occurrence of winter aggregations in tropical P. versicolor functions to allow some females to wait for better environmental conditions to start a new nest, rather than all being obliged to start a new nest as soon as they emerge.

  5. "Winter" aggregations, colony cycle, and seasonal phenotypic change in the paper wasp Polistes versicolor in subtropical Brazil.

    Science.gov (United States)

    Gobbi, Nivar; Noll, Fernando B; Penna, Marcelo A H

    2006-10-01

    Social wasps from temperate zones have clear annual colony cycles, and the young queens hibernate during winter. In the subtropics, the only previously reported evidence for the existence of "hibernation" is the facultative winter aggregations of females during harsh climate conditions. As in temperate-zone species analyzed so far, we show in this study that in the paper wasp, Polistes versicolor, a subtropical species, body size increases as an unfavorable season approaches. Our morphological studies indicate that larger females come from winter aggregations--that is, they are new queens. Multivariate analyses indicate that size is the only variable analyzed that shows a relationship to the differences. Given the absence of a harsh climate, we suggest that the occurrence of winter aggregations in tropical P. versicolor functions to allow some females to wait for better environmental conditions to start a new nest, rather than all being obliged to start a new nest as soon as they emerge. PMID:16896975

  6. Biodegradation of pesticide triclosan by A. versicolor in simulated wastewater and semi-synthetic media.

    Science.gov (United States)

    Ertit Taştan, Burcu; Dönmez, Gönül

    2015-02-01

    Triclosan is known as an antimicrobial agent, a powerful bacteriostat and an important pesticide. In this paper biodegradation of triclosan by Aspergillus versicolor was investigated. Effects of simulated wastewater and semi-synthetic media on fungal triclosan degradation process were detected. HPLC analysis showed that fungal triclosan biodegradation yield was 71.91% at about 7.5 mg/L concentration in semi-synthetic medium and was 37.47% in simulated wastewater. Fungus could be able to tolerate the highest triclosan concentration (15.69 mg/L). The biodegradation yield was 29.81% and qm was 2.22 mg/g at this concentration. Some of the parameters, such as pH, culture media, increasing triclosan and biomass concentrations were optimized in order to achieve the effective triclosan biodegradation process. The highest triclosan biodegradation yields of all microorganisms were achieved by A. versicolor.

  7. Non conventional biological treatment based on Trametes versicolor for the elimination of recalcitrant anticancer drugs in hospital wastewater

    OpenAIRE

    Ferrando Climent, Laura; Cruz Morató, Carles; Marco Urrea, Ernest; Vicent, Teresa; Sarrà i Adroguer, Montserrat; Rodríguez Mozaz, Sara; Barceló i Cullerés, Damià

    2015-01-01

    This work presents a study about the elimination of anticancer drugs, a group of pollutants considered recalcitrant during conventional activated sludge wastewater treatment, using a biological treatment based on the fungus Trametes versicolor. A 10-L fluidized bed bioreactor inoculated with this fungus was set up in order to evaluate the removal of 10 selected anticancer drugs in real hospital wastewater. Almost all the tested anticancer drugs were completely removed from the wastewater at ...

  8. Detection of Malassezia Species Isolated From Patients With Pityriasis Versicolor and Seborrheic Dermatitis Using Nested-PCR

    Directory of Open Access Journals (Sweden)

    Zarei Mahmoudabadi

    2014-12-01

    Full Text Available Background The species of the genus Malassezia are lipophilic and dimorphic yeasts that are regarded as part of the normal flora of the skin of humans and warm-blooded animals. These organisms are the cause of superficial mycosis in humans and other animals, and are common in pityriasis versicolor and seborrheic dermatitis. Objectives The purpose of this study was to determine the frequency of common Malassezia species in patients affected by pityriasis versicolor and seborrheic dermatitis using of the nested PCR method, in the city of Ahvaz. Patients and Methods In the present study, 85 samples from patients with pityriasis versicolor and seborrheic dermatitis were analyzed by the nested-PCR method. During the first stage, the internal transcribed spacer (ITS region from the ribosomal DNA was reproduced using primers ITS4-R and ITS1F-N. During the second stage, the product of the first step was used as DNA and using three special primer pairs, including Mf-F, 5.8SR and M.gl-F, 5.8SR and M.rt-F and M.rt-R, the inner part of the first phase was detected. Results The most common isolate was Malassezia furfur (51.3% followed by M. globosa (35.2% and M. restricta (13.5%. Amongst the 30 patients with seborrheic dermatitis, in 15 cases (65.2% M. restricta, in six cases (26.1% M. globosa and in two cases (8.7% M. furfur was detected and in seven patients no isolate was detected. Conclusions The nested-PCR is a rapid and repeatable method for identification of important Malassezia species and this method is recommended for use on more patients. In addition the most common agents of pityriasis versicolor and seborrheic dermatitis were M. furfur and M. restricta, respectively.

  9. Lignin Peroxidase Activity Is Not Important in Biological Bleaching and Delignification of Unbleached Kraft Pulp by Trametes versicolor

    OpenAIRE

    Archibald, Frederick S.

    1992-01-01

    The discovery in 1983 of fungal lignin peroxidases able to catalyze the oxidation of nonphenolic aromatic lignin model compounds and release some CO2 from lignin has been seen as a major advance in understanding how fungi degrade lignin. Recently, the fungus Trametes versicolor was shown to be capable of substantial decolorization and delignification of unbleached industrial kraft pulps over 2 to 5 days. The role, if any, of lignin peroxidase in this biobleaching was therefore examined. Sever...

  10. Walking to survive. Searching, feeding and egg production of the carabid beetle Pterostichus coerulescens L. (= Poecilus versicolor Sturm).

    OpenAIRE

    Mols, P.J.M.

    1993-01-01

    This study concerns the prey-searching and feeding behaviour of the polyphagous groundbeetle Pterostichus coerulescens L. ( = Poecilus versicolor Sturm), a common species on sandy soils. This ground beetle rarely flies, thus preysearching behaviour involves walking. The beetle is diurnal. As object of research, predators of this kind are very suitable because they can be handled easily, their behaviour can be observed directly or filmed with a video camera. Furthermore they are abundantly ava...

  11. Continuous degradation of a mixture of sulfonamides by Trametes versicolor and identification of metabolites from sulfapyridine and sulfathiazole

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez-Rodriguez, Carlos E., E-mail: CarlosEsteban.Rodriguez@uab.cat [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 2060 San Jose (Costa Rica); Jesus Garcia-Galan, Ma. [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034, Barcelona (Spain); Blanquez, Paqui [Departament d' Enginyeria Quimica, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Diaz-Cruz, M. Silvia [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034, Barcelona (Spain); Barcelo, Damia [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034, Barcelona (Spain); Catalan Institute for Water Research (ICRA), Parc Cientific i Tecnologic de la Universitat de Girona, C/Emili Grahit, 101 Edifici H2O, E-17003 Girona (Spain); Caminal, Gloria [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Vicent, Teresa [Departament d' Enginyeria Quimica, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain)

    2012-04-30

    Highlights: Black-Right-Pointing-Pointer Degradation of sulfapyridine and sulfathiazole by Trametes versicolor was evaluated. Black-Right-Pointing-Pointer The role of laccase and cytochrome P450 was determined. Black-Right-Pointing-Pointer Degradation metabolites were identified for sulfapyridine (8) and sulfathiazole (5). Black-Right-Pointing-Pointer A mixture of three sulfonamides was degraded in a continuous fluidized bed reactor. - Abstract: In this study, we assessed the degradation of the sulfonamides sulfapyridine (SPY) and sulfathiazole (STZ) by the white-rot fungus Trametes versicolor. Complete degradation was accomplished in fungal cultures at initial pollutant concentrations of approximately 10 mg L{sup -1}, although a longer period of time was needed to completely remove STZ in comparison to SPY. When cytochrome P450 inhibitors were added to the fungal cultures, STZ degradation was partially suppressed, while no additional effect was observed for SPY. Experiments with purified laccase and laccase mediators caused the removal of greater than 75% of each antibiotic. Ultra-performance liquid chromatography-quadupole time of flight mass spectrometry (UPLC-QqTOF-MS) analyses allowed the identification of a total of eight degradation intermediates of SPY in both the in vivo and the laccase experiments, being its desulfonated moiety the commonly detected product. For STZ, a total of five products were identified. A fluidized bed reactor with T. versicolor pellets degraded a mixture of sulfonamides (SPY, STZ and sulfamethazine, SMZ) by greater than 94% each at a hydraulic residence time of 72 h. Because wastewater contains many diverse pollutants, these results highlight the potential of T. versicolor as a bioremediation agent not only for the removal of antibiotics but also for the elimination of a wide range of contaminants.

  12. Discovery of novel xylosides in co-culture of basidiomycetes Trametes versicolor and Ganoderma applanatum by integrated metabolomics and bioinformatics.

    Science.gov (United States)

    Yao, Lu; Zhu, Li-Ping; Xu, Xiao-Yan; Tan, Ling-Ling; Sadilek, Martin; Fan, Huan; Hu, Bo; Shen, Xiao-Ting; Yang, Jie; Qiao, Bin; Yang, Song

    2016-01-01

    Transcriptomic analysis of cultured fungi suggests that many genes for secondary metabolite synthesis are presumably silent under standard laboratory condition. In order to investigate the expression of silent genes in symbiotic systems, 136 fungi-fungi symbiotic systems were built up by co-culturing seventeen basidiomycetes, among which the co-culture of Trametes versicolor and Ganoderma applanatum demonstrated the strongest coloration of confrontation zones. Metabolomics study of this co-culture discovered that sixty-two features were either newly synthesized or highly produced in the co-culture compared with individual cultures. Molecular network analysis highlighted a subnetwork including two novel xylosides (compounds 2 and 3). Compound 2 was further identified as N-(4-methoxyphenyl)formamide 2-O-β-D-xyloside and was revealed to have the potential to enhance the cell viability of human immortalized bronchial epithelial cell line of Beas-2B. Moreover, bioinformatics and transcriptional analysis of T. versicolor revealed a potential candidate gene (GI: 636605689) encoding xylosyltransferases for xylosylation. Additionally, 3-phenyllactic acid and orsellinic acid were detected for the first time in G. applanatum, which may be ascribed to response against T.versicolor stress. In general, the described co-culture platform provides a powerful tool to discover novel metabolites and help gain insights into the mechanism of silent gene activation in fungal defense. PMID:27616058

  13. Long term storage of Pleurotus ostreatus and Trametes versicolor isolates using different cryopreservation techniques and its impact on laccase activity.

    Science.gov (United States)

    Eichlerová, Ivana; Homolka, Ladislav; Tomšovský, Michal; Lisá, Ludmila

    2015-12-01

    The strain Pleurotus ostreatus Florida f6, its 45 basidiospore-derived isolates (both monokaryons and dikaryons prepared in our laboratory), Trametes versicolor strain CCBAS 614 and 22 other T. versicolor isolates obtained from the sporocarps collected in distant localities were successfully preserved for 12 y using perlite and straw cryopreservation protocols. All tested isolates survived a 12-year storage in liquid nitrogen (LN) and their laccase production and Poly B411 decolorization capacity was preserved. Also mycelium extension rate and the types of colony appearance of individual isolates remained unchanged. Different cryopreservation techniques were also tested for the short time (24 h) and the long time (6 m) storage of the culture liquid with extracellular laccase produced by T. versicolor strain CCBAS 614. The results showed that 10 % glycerol was the most suitable cryopreservant. The absence of the cryopreservant did not cause high loss of laccase activity in the samples; the presence of DMSO (5 or 10 %) in LN-stored samples caused mostly a decrease of laccase activity. For the preservation of laccase activity in the liquid culture the storage in the freezer at -80 °C is more convenient than the storage in liquid nitrogen.

  14. Degradation of the drug sodium diclofenac by Trametes versicolor pellets and identification of some intermediates by NMR

    International Nuclear Information System (INIS)

    Degradation of diclofenac sodium, a nonsteroidal anti-inflammatory drug widely found in the aquatic environment, was assessed using the white-rot fungus Trametes versicolor. Almost complete diclofenac removal (≥94%) occurred the first hour with T. versicolor pellets when the drug was added at relatively high (10 mg L-1) and environmentally relevant low (45 μg L-1) concentrations in a defined liquid medium. In vivo and in vitro experiments using the cytochrome P450 inhibitor 1-aminobenzotriazole and purified laccase, respectively, suggested at least two different mechanisms employed by T. versicolor to initiate diclofenac degradation. Two hydroxylated metabolites, 4'-hydroxydiclofenac and 5-hydroxydiclofenac, were structurally elucidated by nuclear magnetic resonance as degradation intermediates in fungal cultures spiked with diclofenac. Both parent compound and intermediates disappeared after 24 h leading to a decrease in ecotoxicity calculated by the Microtox test. Laccase-catalyzed transformation of diclofenac led to the formation of 4-(2,6-dichlorophenylamino)-1,3-benzenedimethanol, which was not detected in in vivo experiments probably due to the low laccase activity levels observed through the first hours of incubation.

  15. Identification of Malassezia Species Isolated from Patients with Pityriasis Versicolor Using PCR-RFLP Method in Markazi Province, Central Iran.

    Directory of Open Access Journals (Sweden)

    Mojtaba Didehdar

    2014-05-01

    Full Text Available The lipophilic yeasts of Malassezia species are members of the normal skin microbial that are cause of pityriasis versicolor. Pityriasis versicolor is a common superficial fungal infection with world-wide distribution. The phenotypic methods for identification of Malassezia species usually are time consuming and unreliable to differentiate newly identified species. But DNA-based techniques rapidly and accurately identified Malassezia species. The purpose of this study was isolation and identification of Malassezia Species from patients with pityriasis versicolor by molecular methods in Markazi Province, Central Iran in 2012.Mycologic examinations including direct microscopy and culture were performed on clinical samples. DNA extraction was performed from colonies. The ITS1 region of rDNA from isolates of Malassezia species were amplified by PCR reaction. The PCR were digested by Cfo I enzyme.From 70 skin samples, were microscopically positive for Malassezia elements, 60 samples were grown on culture medium (85.7%. Using PCR-RFLP method, that was performed on 60 isolates, 37(61.6% M. globosa, 14(23.3% M. furfur, 5(8.4% M. sympodialis and 4(6.7% M. restrictawere identified. In one case was isolated M. globosa along with M. restricta.The PCR-RFLP method is a useful and reliable technique for identification of differentiation of Malas-sezia species.

  16. Identification of Malassezia species isolated from patients with seborrhoeic dermatitis, atopic dermatitis, pityriasis versicolor and normal subjects.

    Science.gov (United States)

    Nakabayashi, A; Sei, Y; Guillot, J

    2000-10-01

    We identified Malassezia species isolated from 42 patients with seborrhoeic dermatitis, 17 patients with atopic dermatitis, 22 patients with pityriasis versicolor, 35 normal subjects and 73 healthy medical students. Regarding the prevalence of Malassezia species in the 35 normal subjects, the frequency of isolation of Malassezia globosa was 22%, M. sympodialis 10% and M. furfur 3%. M. slooffiae, M. pachydermatis, M. restricta and M. obtusa were infrequently isolated from normal skin. Two different species were isolated coincidentally from seven samples. In the patients with atopic dermatitis, M. furfur was isolated more frequently from lesional skin (21%) than non-lesional skin (11%). However, there was no statistical significance. Therefore, this result, by itself, is insufficient to prove that M. furfur should be considered to be an exacerbating factor of atopic dermatitis. In seborrhoeic dermatitis, M. furfur (35%) and M. globosa (22%) were isolated from lesional skin on the face at significantly high rates in comparison with the normal subjects. Therefore, M. furfur and/or M. globosa may be pathogens of seborrhoeic dermatitis. M. globosa was isolated at a frequency of 55% from lesional skin of pityriasis versicolor, while all other species were below 10%. These data suggest that the pathogenic species of pityriasis versicolor is M. globosa.

  17. [Levels of selenium in urine after treatment of pityriasis versicolor with a 1.0% selenium sulfide].

    Science.gov (United States)

    Costa Martins, J E; Macedo De Souza, E; Salebian, A; Sampaio, S A

    1976-01-01

    The treatment of pityriasis versicolor by the topical application of selenium sulfide 1% was studied in two groups of patients. Diagnosis and response to therapy was determined by clinical observation. Wood's light fluorescence, and direct microscopic examination. The efficacy of three different therapeutic regimes was studied in one group by the application of the drug to the entire skin for either five minutes, fifteen minutes, or twelve hours for eighteen days. Each method proved equally effective in resolving the infection. Therefore, the cutaneous application of selenium sulfide 1 % for five minutes daily for eighteen days is the recommended treatment for pityriasis versicolor. The percutaneous absorption of selenium sulfide was also studied in another groups who applied the drug to the entire skin for five minutes for eighteen days. Fluorimetric analysis of urinary samples collected on the third and thirteenth days of treatment revealed no significant increase in the excretion of selenium as compared to pretreatment levels. Systemic toxicity was not observed in any of the patients treated. The results suggest that the selenium sulfide is absorbed poorly from the skin and is a safe and effective therapy for pityriasis versicolor.

  18. Randomized comparative clinical trial of artemisia sieberi 5% lotion and clotrimazole 1% lotion for the treatment of pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Rad Farrokh

    2008-01-01

    Full Text Available Aims: To compare the therapeutic effects of topical Artremisia sieberi 5% lotion with topical clotrimazole 1% lotion in the treatment of pityriasis versicolor. Materials and Methods: 100 patients with pityriasis versicolor and microscopic identification of Malassezia furfur were randomly assigned to treatment with either topical Artemisia sieberi 5% lotion (group 1 or topical clotrimazole 1% lotion (group 2 for 2 weeks. Group 1 and group 2 consisted of 51 and 49 patients respectively. The patients were evaluated both clinically and mycologically at baseline and every 2 weeks for a period of 4 weeks. Results: At the end of the second week, clinical cure rates were 86.3% and 65.3% for group 1 and group 2 respectively ( P < 0.01, but at the same time mycological cure rate was 92.2% for group 1 and 73.5% for group 2 ( P < 0.05. At the end of the fourth week, clinical cure rates were 86.3% and 59.2% for group 1 and group 2 respectively ( P < 0.01, and at the same time mycologic cure rate was 96.1% for group 1 and 65.3% for group 2 ( P < 0.01. Conclusions: The results of this study demonstrated that Artemisia sieberi 5% lotion was more effective than clotrimazole 1% lotion in the treatment of pityriasis versicolor.

  19. A reappraisal of the role of Pityrosporum orbiculare in pityriasis versicolor and the significance of extracellular lipase.

    Science.gov (United States)

    Catterall, M D; Ward, M E; Jacobs, P

    1978-12-01

    Pityrosporum orbiculare is an obligate lipophilic yeast in vitro, which suggests it possesses an extracellular lipase crucial for nutrition. If present in vivo, the enzyme would enable the yeast to utilize skin surface lipids, which may therfore play an important role in the pathogenesis of pityriasis versicolor. Cultured P. orbiculare and biopsy material from patients with pityriasis versicolor were investigated for the presence of lipase by electron microscope histochemistry. At sites of lipase activity, fatty acid hydrolyzed from Tween 80 substrate reacts with Ca++ ions to form an insoluble Ca++ soap. Exchange of Ca++ with Pb++ enables the sites of lipase activity to be visualized as electron dense deposits of insoluble lead soap. Surface lipase activity was apparent when the technique was applied to P. orbiculare grown on lipid containing medium and its specificity confirmed by removal of substrate and inhibition by di-isopropyl fluorophosphate and quinine hydrochloride, but not by sodium fluoride. When the same technique was applied to stratum corneum infected with Pityrosporum furfur (Malassez), no reaction product could be detected. It is postulated that lipase, although critical for fungal nutrition in vitro, is unlikely to be of importance in vivo. Skin surface lipids are therefore probably not relevant to the pathogenesis of pityriasis versicolor.

  20. Flutrimazole shampoo 1% versus ketoconazole shampoo 2% in the treatment of pityriasis versicolor. A randomised double-blind comparative trial.

    Science.gov (United States)

    Rigopoulos, D; Gregoriou, S; Kontochristopoulos, G; Ifantides, A; Katsambas, A

    2007-05-01

    Flutrimazole is an imidazole derivative that has been proven to be efficient in superficial skin fungal infections. The aim of this randomised double-blind study was to compare for the first time, the efficiency and safety of flutrimazole 1% shampoo versus ketoconazole 2% shampoo in the treatment of tinea versicolor. Study population consisted of 60 patients with pityriasis versicolor diagnosed clinically and through direct microscopy and culture. Patients were randomly assigned to two groups: one instructed to apply flutrimazole shampoo 1% and one instructed to apply ketoconazole shampoo 2% both on head and body for 14 days. Patients were re-evaluated 14 days after the end of treatment clinically and through direct microscopy and culture. Twenty-one of 26 patients (80.8%) in the ketoconazole and 22 of 29 patients (75.9%) in the flutrimazole group had both visual healing and negative mycological evaluation. Comparison of the response between the two groups with the Yates' corrected chi-square was found statistically not significant (chi(2) = 0.19, d.f. = 1, P = 0.91). None of the patients in the two groups reported any adverse effects. Fourteen (53%) patients in the ketoconazole group and 23 (79%) in the flutrimazole group assessed the shampoos as cosmetically acceptable regarding texture, smell and foam properties. Flutrimazole shampoo 1% appears to present efficacy comparable with ketoconazole 2% in the treatment of tinea versicolor.

  1. Removal of pharmaceuticals, polybrominated flame retardants and UV-filters from sludge by the fungus Trametes versicolor in bioslurry reactor

    International Nuclear Information System (INIS)

    Highlights: ► Sludge from a WWTP was treated in a fungal slurry reactor with Trametes versicolor. ► Twenty-four pharmaceuticals were removed at important extents. ► UV-filters and brominated flame retardants were also degraded. ► Overall toxicity of sludge increased despite the pollutant removal. - Abstract: Conventional wastewater treatments are inefficient in the removal of many organic pollutants. The presence of these contaminants in the final sludge represents a source of environmental pollution due to the increasing use of biosolids in land application. A biotechnological approach which employed the fungus Trametes versicolor in a sludge-bioslurry reactor was assessed in order to remove several groups of emerging pollutants. Biological fungal activity was monitored by means of ergosterol and laccase determinations. Fifteen out of 24 detected pharmaceuticals were removed at efficiencies over 50% after the treatment, including eight completely degraded. Removal ranged between 16–53% and 22–100% for the brominated flame retardants and the UV-filters, respectively. Only two of all the detected compounds remained unchanged after the treatment. Although elimination results are promising, the toxicity of the final sludge increased after the treatment. This finding is contrary to the toxicity results obtained in similar treatments of sludge with T. versicolor in solid-phase.

  2. Removal of pharmaceuticals, polybrominated flame retardants and UV-filters from sludge by the fungus Trametes versicolor in bioslurry reactor

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez-Rodriguez, Carlos E. [Unitat Asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 2060 San Jose (Costa Rica); Baron, Enrique; Gago-Ferrero, Pablo; Jelic, Aleksandra; Llorca, Marta; Farre, Marinella; Diaz-Cruz, M. Silvia; Eljarrat, Ethel [Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona (Spain); Petrovic, Mira [Institucio Catalana de Reserca i Estudis Avancats (ICREA), Passeig Lluis Companys 23, 80010 Barcelona (Spain); Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona (Spain); Caminal, Gloria, E-mail: Gloria.Caminal@uab.cat [Unitat Asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Barcelo, Damia [Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona (Spain); Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona (Spain); King Saud University (KSU), P.O. Box 2455, 11451 Riyadh (Saudi Arabia); and others

    2012-09-30

    Highlights: Black-Right-Pointing-Pointer Sludge from a WWTP was treated in a fungal slurry reactor with Trametes versicolor. Black-Right-Pointing-Pointer Twenty-four pharmaceuticals were removed at important extents. Black-Right-Pointing-Pointer UV-filters and brominated flame retardants were also degraded. Black-Right-Pointing-Pointer Overall toxicity of sludge increased despite the pollutant removal. - Abstract: Conventional wastewater treatments are inefficient in the removal of many organic pollutants. The presence of these contaminants in the final sludge represents a source of environmental pollution due to the increasing use of biosolids in land application. A biotechnological approach which employed the fungus Trametes versicolor in a sludge-bioslurry reactor was assessed in order to remove several groups of emerging pollutants. Biological fungal activity was monitored by means of ergosterol and laccase determinations. Fifteen out of 24 detected pharmaceuticals were removed at efficiencies over 50% after the treatment, including eight completely degraded. Removal ranged between 16-53% and 22-100% for the brominated flame retardants and the UV-filters, respectively. Only two of all the detected compounds remained unchanged after the treatment. Although elimination results are promising, the toxicity of the final sludge increased after the treatment. This finding is contrary to the toxicity results obtained in similar treatments of sludge with T. versicolor in solid-phase.

  3. Identificação de espécies de Malassezia em pacientes com pitiríase versicolor em Goiânia-GO Identification of Malassezia species in patients with pityriasis versicolor in Goiânia-GO

    Directory of Open Access Journals (Sweden)

    Karla Carvalho Miranda

    2006-12-01

    Full Text Available O objetivo deste estudo foi verificar a freqüência de pitiríase versicolor e identificar leveduras do gênero Malassezia, de pacientes encaminhados ao laboratório de Micologia da Universidade Federal de Goiás em Goiânia. Foram diagnosticados 95 casos de pitíriase versicolor e identificados quatro espécies de Malassezia: Malassezia furfur, Malassezia sympodialis, Malassezia globosa e Malassezia obtusa.The objectives of this study were to investigate the frequency of pityriasis versicolor occurrence and to identify yeasts of the genus Malassezia in patients at the mycology laboratory of the Federal University of Goiás, in Goiânia, State of Goiás. Ninety-five cases of pityriasis versicolor were diagnosed, and four species of Malassezia were identified: Malassezia furfur, Malassezia sympodialis, Malassezia globosa and Malassezia obtusa.

  4. Treatment of a Textile Effluent from Dyeing with Cochineal Extracts Using Trametes versicolor Fungus

    Directory of Open Access Journals (Sweden)

    Gabriela Arroyo-Figueroa

    2011-01-01

    Full Text Available Trametes versicolor (Tv fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. However, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. Experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of Tv fungus were done. Treatments to determine decolorization in the presence or absence of Kirk's medium, glucose, and fungus, with an addition of 50% (v v-1 of nonsterilized effluent were performed. Physicochemical characterization was performed at the start and end of the treatment. Degradation kinetics were determined. A direct relationship was found between the dry weight of fungi, pH, and the decolorization system, with higher decolorization at lower pH levels (pH ~4.3. High decolorization (81% ± 0.09; 88% ± 0.17; and 99% ± 0.04 for three of the eight treatments (Kirk's medium without glucose, Kirk's medium with glucose, and without medium with glucose, respectively was found. Toxicity tests determined an increase in the initial effluent toxicity (7.33 TU compared with the final treatment (47.73 TU in a period of 11 days. For this system, a degradation sequence of the carminic acid structure present in the effluent by the Tv fungus is suggested, in which it is seen that metabolites still containing aromatic structures are generated.

  5. Investigation of the toxicity of the products of decoloration of Amaranth by Trametes versicolor.

    Science.gov (United States)

    Gavril, Mihaela; Hodson, Peter V

    2007-01-01

    Trametes versicolor decolorized 2000 mg L(-1) of the mono-azo substituted naphthalenic dye Amaranth with no dye sorption observed visually. The changes in the toxicity were assessed over a period of 30 d for the dye-treated viable culture, control (no dye added), and a boiled culture treated with dye, using the Microtox Acute Toxicity assay. Before dye addition, the culture filtrate had some toxicity, which increased after the dye addition. The toxicity of the dye-treated culture decreased during the treatment. The loss of toxicity occurred at the same time, with the loss of color suggesting that detoxification is associated with decoloration. The change in pH was due to natural metabolic processes and had a small effect on detoxification. Because the toxicity of the treatment was similar to that of the control at the end of the treatment, the effluent seems to be safe for release into the environment, potentially rendering this treatment suitable for industrial application.

  6. Pityriasis versicolor during anti-TNF-α monoclonal antibody therapy: therapeutic considerations.

    Science.gov (United States)

    Balestri, Riccardo; Rech, Giulia; Piraccini, Bianca Maria; Antonucci, Angela; Ismaili, Alma; Patrizi, Annalisa; Bardazzi, Federico

    2012-09-01

    Anecdotal reports have shown that tumour necrosis factor (TNF)-α inhibition may cause unchecked superficial infection with the microorganisms responsible for pityriasis versicolor (PV). We observed several cases of PV, which is frequently resistant to topical therapies, in psoriatic patients undergoing anti-TNF-α monoclonal antibody therapy. To evaluate the incidence and the therapeutic management of PV in this group of individuals, between 1 January and 27 December 2010, we examined 153 psoriatic patients for the hypopigmented/hyperpigmented macular and scaling lesions associated with PV. All patients positive for PV were given topical therapy with miconazole nitrate cream twice daily for 28 days, after which they were re-evaluated. In patients non-responsive to topical therapy, we started systemic therapy with fluconazole, 300 mg week(-1) for 3 weeks. We diagnosed seven cases of PV. At the end of topical treatment, complete healing of lesions was observed in only one patient. In the other six patients, systemic treatment led to complete resolution of the infection. Although the onset of PV during anti-TNF-α therapy is seldom reported, it is not likely to be rare, but rather under-reported because of its limited pathological significance. In our opinion, the therapeutic management of this condition deserves greater consideration, as the use of topical treatments alone is largely ineffective compared with systemic treatment.

  7. Efficient removal of disperse dye by mixed culture of ganoderma lucidum and coriolus versicolor

    International Nuclear Information System (INIS)

    In the current study, an attempt was made to check the potential of aerobic mixed culture of two indigenous white rot fungi for the decolorization of different disperse dyes in batch culture mode and optimization of different conditions to enhance the biotransformation of dyes. Initial screening trial with six disperse dyes, viz. (Foron Yellow RD5GL, Foron Red RDRBLS, Foron Rubine RDGFL, Foron Black RD3GRN, Foron Blue RDGLN and Foron Turquoise SBLN), was carried out using mixed culture of Ganoderma lucidum and Coriolus versicolor. From all the tested dyes, the mixed culture showed better removal efficiency (93 %) with Foron Turquoise SBLN dye after 8 days of incubation period as compared to other tested dyes. Enhanced color removal (98 %) was observed when the medium was amended by ammonium tartarate, maltose, MnSO/sub 4/ at pH 4.5 and 30 degree C with 2 mL fungal culture during 2nd day of incubation period. Enzyme profile showed that the mixed culture produced three liginolytic enzymes like lignin peroxidase (LiP), manganase peroxidase (MnP) and laccase but MnP was found to be the major enzyme. The results indicated that white rot fungi (WRF) could be used to treat wastewater containing disperse dyes. (author)

  8. Fungal biodegradation of lignopolystyrene graft copolymers. [Pleurotus ostreatus; Phanerochaete chrysosporium; Trametes versicolor; Gloeophyllum trabeum

    Energy Technology Data Exchange (ETDEWEB)

    Milstein, O.; Gersonde, R.; Huttermann, A. (Forstbotanisches Inst. der Univ. Gottingen (Germany)); MengJiu Chen; Meister, J.J (Univ. of Detroit Mercy, MI (United States))

    1992-10-01

    White rot basidiomycetes were able to biodegrade styrene (1-phenylethene) graft copolymers of lignin containing different proportions of lignin and polystyrene (poly(1-phenylethylene)). The biodegradation tests were run on lignin-styrene copolymerization products which contained 10.3, 32.2, and 50.4{percent} (wt/wt) lignin. The polymer samples were incubated with the white rot fungi Pleurotus ostreatus, Phanerochaete chrysosporium, and Trametes versicolor and the brown rot fungus Gloeophyllum trabeum. White rot fungi degraded the plastic samples at a rate which increased with increasing lignin content in the copolymer sample. Both polystyrene and lignin components of the copolymer were readily degraded. Polystyrene pellets were not degradable in these tests. Degradation was verified for both incubated and control samples by weight loss, quantitative UV spectrophotometric analysis of both lignin and styrene residues, scanning electron microscopy of the plastic surface, and the presence of enzymes active in degradation during incubation. Brown rot fungus did not affect any of the plastics. White rot fungi produced and secreted oxidative enzymes associated with lignin degradation in liquid media during incubation with lignin-polystyrene copolymer.

  9. Flavonoid C- and O-glycosides from the Mongolian medicinal plant Dianthus versicolor Fisch.

    Science.gov (United States)

    Obmann, Astrid; Werner, Ingrid; Presser, Armin; Zehl, Martin; Swoboda, Zita; Purevsuren, Sodnomtseren; Narantuya, Samdan; Kletter, Christa; Glasl, Sabine

    2011-09-27

    Eighteen flavonoids were identified from an aqueous extract of the aerial parts of Dianthus versicolor, a plant used in traditional Mongolian medicine against liver diseases. The flavonoid C- and O-glycosides isoorientin-7-O-rutinoside, isoorientin-7-O-rhamnosyl-galactoside, isovitexin-7-O-rutinoside, isovitexin-7-O-rhamnosyl-galactoside, isoscoparin-7-O-rutinoside, isoscoparin-7-O-rhamnosyl-galactoside, isoscoparin-7-O-galactoside, and isoorientin-7-O-galactoside were isolated and structurally elucidated. Their structures were established on the basis of extensive spectroscopic techniques including LC-UV-DAD, LC-MS(n), LC-HRMS, 1D and 2D NMR spectroscopy, and by GC-MS analysis after hydrolysis. Flavonoids with such a high glycosylation pattern are rare within the genus Dianthus. Furthermore, isovitexin-7-O-glucoside (saponarin), isovitexin-2″-O-rhamnoside, apigenin-6-glucoside (isovitexin), luteolin-7-O-glucoside, apigenin-7-O-glucoside, as well as the aglycons luteolin, apigenin, chrysoeriol, diosmetin, and acacetin were identified by TLC and LC-DAD-MS(n) in comparison to reference substances or literature data. The NMR data of seven structures have not been reported in the literature to date.

  10. Treatment of a textile effluent from dyeing with cochineal extracts using Trametes versicolor fungus.

    Science.gov (United States)

    Arroyo-Figueroa, Gabriela; Ruiz-Aguilar, Graciela M L; López-Martínez, Leticia; González-Sánchez, Guillermo; Cuevas-Rodríguez, Germán; Rodríguez-Vázquez, Refugio

    2011-01-01

    Trametes versicolor (Tv) fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. However, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. Experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of Tv fungus were done. Treatments to determine decolorization in the presence or absence of Kirk's medium, glucose, and fungus, with an addition of 50% (v v-1) of nonsterilized effluent were performed. Physicochemical characterization was performed at the start and end of the treatment. Degradation kinetics were determined. A direct relationship was found between the dry weight of fungi, pH, and the decolorization system, with higher decolorization at lower pH levels (pH ~4.3). High decolorization (81% ± 0.09; 88% ± 0.17; and 99% ± 0.04) for three of the eight treatments (Kirk's medium without glucose, Kirk's medium with glucose, and without medium with glucose, respectively) was found. Toxicity tests determined an increase in the initial effluent toxicity (7.33 TU) compared with the final treatment (47.73 TU) in a period of 11 days. For this system, a degradation sequence of the carminic acid structure present in the effluent by the Tv fungus is suggested, in which it is seen that metabolites still containing aromatic structures are generated. PMID:21552764

  11. In Vitro Antileishmanial Activity of Sterols from Trametes versicolor (Bres. Rivarden).

    Science.gov (United States)

    Leliebre-Lara, Vivian; Monzote Fidalgo, Lianet; Pferschy-Wenzig, Eva-Maria; Kunert, Olaf; Nogueiras Lima, Clara; Bauer, Rudolf

    2016-01-01

    Two ergostanes, 5α,8α-epidioxy-22E-ergosta-6,22-dien-3β-ol (1) and 5α-ergost-7,22-dien-3β-ol (2), and a lanostane, 3β-hydroxylanostan-8,24-diene-21-oic acid (trametenolic acid) (3), were isolated from an n-hexane extract prepared from the fruiting body of Trametes versicolor (Bres. Rivarden). The activity of the isolated sterols was evaluated against promastigotes and amastigotes of Leishmania amazonensis Lainson and Shaw, 1972. The lanostane, compound (3), showed the best inhibitory response (IC50 promastigotes 2.9 ± 0.1 μM and IC50 amastigotes 1.6 ± 0.1 μM). This effect was 25-fold higher compared with its cytotoxic effect on peritoneal macrophages from BALB/c mice. Therefore, trametenolic acid could be regarded as a promising lead for the synthesis of compounds with antileishmanial activity. PMID:27517895

  12. A multivariate analysis of genetic variation in the advertisement call of the gray treefrog, Hyla versicolor.

    Science.gov (United States)

    Welch, Allison M; Smith, Michael J; Gerhardt, H Carl

    2014-06-01

    Genetic variation in sexual displays is crucial for an evolutionary response to sexual selection, but can be eroded by strong selection. Identifying the magnitude and sources of additive genetic variance underlying sexually selected traits is thus an important issue in evolutionary biology. We conducted a quantitative genetics experiment with gray treefrogs (Hyla versicolor) to investigate genetic variances and covariances among features of the male advertisement call. Two energetically expensive traits showed significant genetic variation: call duration, expressed as number of pulses per call, and call rate, represented by its inverse, call period. These two properties also showed significant genetic covariance, consistent with an energetic constraint to call production. Combining the genetic variance-covariance matrix with previous estimates of directional sexual selection imposed by female preferences predicts a limited increase in call duration but no change in call rate despite significant selection on both traits. In addition to constraints imposed by the genetic covariance structure, an evolutionary response to sexual selection may also be limited by high energetic costs of long-duration calls and by preferences that act most strongly against very short-duration calls. Meanwhile, the persistence of these preferences could be explained by costs of mating with males with especially unattractive calls. PMID:24621402

  13. A double-blind comparison of 2% ketoconazole and 1% clotrimazole in the treatment of pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Balwada R

    1996-01-01

    Full Text Available Forty adult patients of pityriasis versicolor were treated with either topical 2% ketoconazole cream (20 patients or topical 1% clotrimazole cream (20 patients. In global assessment of treatment after 2 weeks, 18 (90% out of 20 patients treated with ketoconazole cream were cured while 2 cases had considerable residual lesions. In clotrimazole treated group, 17 (85% out of 20 patients were cured and 3 still had considerable lesions. No significant difference was observed in response rates in the two groups. No side effects were reported in either groups.

  14. 萎缩性花斑糠疹%Atrophying pityriasis versicolor

    Institute of Scientific and Technical Information of China (English)

    胡燕; 朱小红; 陈浩; 张海平; 吉津; 杨莉佳

    2013-01-01

    A 26-year-old woman presented with scaly atrophic erythema on the chest and abdomen for nearly 2 years.Direct microscopic examination of the lesion scrapings revealed numerous stubby hyphae and clustered spores.Periodic acid-Schiff (PAS) staining of the biopsy tissue showed the presence of numerous hyphae and spores in the horny layer.The stain for elastic fibers revealed that the number of elastic fibers decreased,and some elastic fibers became fine and disrupted.The patient was diagnosed with atrophying pityriasis versicolor.The lesions subsided and direct microscopic examination was negative after 1-week treatment with oral itraconazole of 200 mg once daily and 4-week treatment with topical 2% sertaconazole cream.%患者女,26岁.因胸腹部鳞屑性红斑伴萎缩近2年就诊.取皮屑直接镜检,见较多粗短的菌丝及成簇孢子.皮损组织病理检查,过碘酸雪夫(PAS)染色见角质层内较多菌丝和孢子.弹力纤维染色示弹力纤维减少,部分弹力纤维纤细、断裂.诊断:萎缩性花斑糠疹.治疗:口服伊曲康唑胶囊200mg/d共1周,外用2%舍他康唑乳膏4周,皮疹消退,真菌直接镜检复查阴性.

  15. Evaluation of pityriasis versicolor in prisoners: A cross-sectional study

    Directory of Open Access Journals (Sweden)

    Salahi-Moghaddam Abdoreza

    2009-01-01

    Full Text Available Background: Pityriasis versicolor (PV is a mild but recurrent superficial fungal infection of stratum corneum. Many predisposing factors such as late teen and young adulthood age, tropical and subtropical climate (and to a lesser extent temperate climate, immunosuppression, malnutrition, use of oral contraceptives, hyperhidrosis, poor hygiene and a few other factors have been proposed for this condition. Aim: Regarding these probable and possible risk factors, we felt that jail is a unique population which can be affected by this infection and therefore we decided to evaluate some aspects of this infection in this population. Methods: In this cross-sectional study in male prisoners of Bandarabbas city, 230 prisoners were randomly selected. One hundred and five (45.67% of them were diagnosed to be infected. They were also evaluated regarding age, duration of being imprisoned, skin color, site of lesion and its pigmentation change and any concomitant clinical manifestation such as pruritis, burning or cebure. Chi-square test or Fisher′s exact test was used for data analysis. Results: Mean age of infected population was 31.62 ± 8.67 years while peak incidence of PV was in 28-32 years age group. The most common color change was hypopigmentation. No case of PV hyperpigmentation was seen. We could not also observe any association between skin color and duration of being imprisoned with the frequency of infection. Conclusion: There seems to be no difference between pattern of infection in the general population and prisoners′ population in the aspects evaluated.

  16. Identification of Malassezia species from pityriasis versicolor lesions with a new multiplex PCR method.

    Science.gov (United States)

    Vuran, Emre; Karaarslan, Aydın; Karasartova, Djursun; Turegun, Buse; Sahin, Fikret

    2014-02-01

    Despite the fact that a range of molecular methods have been developed as tools for the diagnosis of Malassezia species, there are several drawbacks associated with them, such as inefficiency of differentiating all the species, high cost, and questionable reproducibility. In addition, most of the molecular methods require cultivation to enhance sensitivity. Therefore, alternative methods eliminating cultivation and capable of identifying species with high accuracy and reliability are needed. Herein, a multiplex polymerase chain reaction (PCR)-based method was especially developed for the detection of eleven Malassezia species. The multiplex PCR was standardized by incorporating a consensus forward primer, along with Malassezia species-specific reverse primers considering the sizes of the PCR products. In the method, the multiplex-PCR primer content is divided into three parts to circumvent the problem of increased nonspecific background resulting from the use of a large number of primers. DNA extraction protocol described by Harju and colleagues was modified using liquid nitrogen instead of -80 °C to break down the yeast membrane. By a modified extraction procedure followed by multiplex PCR and electrophoresis, the method enables identification and differentiation of Malassezia species from both of the samples obtained directly from skin and yeast colonies grown in culture. Fifty-five patients who were confirmed with pityriasis versicolor were enrolled in the study. Multiplex PCR detected and differentiated all 55 samples obtained directly from the patients' skin. However, 50 out of 55 samples yielded Malassezia colony in the culture. In addition, eight of 50 colonies were misdiagnosed or not completely differentiated by conventional methods based on the sequence analysis of eight colonies. The method is capable of identifying species with high accuracy and reliability. In addition, it is simple, quick, and cost-effective. More importantly, the method works

  17. Influence of treatment conditions on the oxidation of micropollutants by Trametes versicolor laccase.

    Science.gov (United States)

    Margot, Jonas; Maillard, Julien; Rossi, Luca; Barry, D A; Holliger, Christof

    2013-09-25

    Many organic compounds present at low concentrations in municipal wastewater, such as various pharmaceuticals and biocides, are recalcitrant in conventional wastewater treatment plants (WWTPs). To improve their biodegradation, oxidoreductase enzymes such as laccases were tested. The goal was to find optimal conditions for the transformation of two anti-inflammatory pharmaceuticals (diclofenac (DFC) and mefenamic acid (MFA)), one biocide (triclosan (TCN)) and one plastic additive (bisphenol A (BPA)) by Trametes versicolor laccase. Experiments were conducted in spiked solutions at different pH values (from 3 to 9), enzyme concentrations (70-1400 Ul(-1)), reaction times (0-26 hours) and temperatures (10, 25 and 40°C) following a Doehlert experimental design. A semi-empirical model was developed to understand better the combined effects of the four factors and to determine optimal values. This model was able to fit well the experimental data (R(2)>0.97) and showed good predictive ability. All four factors had a significant effect on the micropollutant oxidation with the greatest influence shown by pH. Results for single compounds were different from those obtained for mixtures of micropollutants. For instance, DFC transformation occurred at much higher rates in mixtures under alkaline conditions. Optimal conditions were compound-dependent, but were found to be between pH 4.5 to 6.5 and between 25°C to more than 40°C. A laccase concentration of 730 Ul(-1) was sufficient to obtain a high removal rate (>90%) of the four individual compounds (range of times: 40 min to 5 hours), showing the potential of laccases to improve biodegradation of environmentally persistent compounds. PMID:23831273

  18. Molecular markers detect cryptic predation on coffee berry borer (Coleoptera: Curculionidae) by silvanid and laemophloeid flat bark beetles (Coleoptera: Silvanidae, Laemophloeidae) in coffee beans

    Science.gov (United States)

    The coffee berry borer, Hypothenemus hampei(Coleoptera: Curculionidae)(Ferrari), is a serious pest of coffee worldwide and has been recently introduced in Hawai’i, first detected in the state in 2010. Adult silvanid flat bark beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and adult laemoph...

  19. Pitiríase versicolor: isolamento e identificação das principais espécies de Malassezia Pityriasis versicolor: isolation and identification of the main species

    Directory of Open Access Journals (Sweden)

    Valéria Maria de Souza Framil

    2010-02-01

    Full Text Available As espécies do gênero Malassezia isoladas foram: Malassezia sympodialis (16,66%, Malassezia furfur (12,50%, Malassezia globosa (11,45% e Malassezia slooffiae (2,10%. A Malassezia sympodialis foi a espécie que predominou em nosso estudo. As espécies de Malassezia identificadas não mostraram correlação com as variantes clínicas e com a distribuição das lesões de pitiríase versicolor quanto às regiões do corpo.Species of the genus Malassezia isolated were: Malassezia sympodialis (16.66%, Malassezia furfur (12.50%, Malassezia globosa (11.45%, and Malassezia slooffiae (2.10%. Malassezia sympodialis predominated in the study. The species of Malassezia identified did not show correlation with clinical variants and with the distribution of pityriasis versicolor lesions in relation to areas of the body.

  20. The Synergistic Beneficial Effects of Ginkgo Flavonoid and Coriolus versicolor Polysaccharide for Memory Improvements in a Mouse Model of Dementia

    Directory of Open Access Journals (Sweden)

    Xianying Fang

    2015-01-01

    Full Text Available This study reports the combination of Ginkgo flavonoid (GF and Coriolus versicolor polysaccharide (CVP in the prevention and treatment of a mouse model of Alzheimer’s disease (AD. GF is a traditional health product, and CVP is the main active ingredient of the medicinal fungus Coriolus versicolor. The Morris water maze test, the Y maze, and the step-through test showed that the combinational use of CVP and GF synergistically improved memory in a mouse model of AD. Based on H&E staining analysis, the combination of CVP and GF decreased the severity of the pathological findings in the brain. Given that the expression of IL-1β, IL-6, and TNF-α was downregulated, the inflammation response in AD mice was considered to be inhibited. The downregulation of GFAP further demonstrated that inflammation was reduced in the brain of AD mice following treatment. Moreover, the expression levels of superoxide dismutase (SOD and catalase (CAT were elevated in the brains of treated mice, indicating that oxidation levels were reduced upon the combination treatment. Our results provide new insights into the efficient utilization of traditional medicine for preventing dementia.

  1. High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

    Science.gov (United States)

    Ottoni, Cristiane; Simões, Marta F.; Fernandes, Sara; Santos, Cledir R.; Lima, Nelson

    2016-01-01

    Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U∙L−1) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs. PMID:27490563

  2. Single-dose oral fluconazole versus topical clotrimazole in patients with pityriasis versicolor: A double-blind randomized controlled trial.

    Science.gov (United States)

    Dehghan, Mohammad; Akbari, Negin; Alborzi, Nazila; Sadani, Somayeh; Keshtkar, Abas A

    2010-08-01

    This study was designed to compare the therapeutic effects of topical clotrimazole and systemic fluconazole in pityriasis versicolor. A double-blind randomized controlled trial was carried out in the dermatological clinic of Gorgan, northern Iran, between April 2006 and May 2007. All consecutive patients with pityriasis versicolor were included and randomly divided into two groups. In the first group (G1), patients underwent treatment with a single dose of fluconazole capsule (400 mg) and placebo cream. In the second group (G2), patients underwent treatment with clotrimazole cream (twice daily) and placebo capsule. The course of treatment was 2 weeks. All subjects were re-evaluated 2, 4 and 12 weeks after the end of the therapeutic course. After 2 weeks, the rate of complete resolution of disease was significantly higher in G2 than G1 (49.1% vs 30%). After 4 weeks, 41 patients (81.2%) of G1 and 52 patients (94.9%) of G2 showed complete resolution. After 12 weeks, 46 patients (92%) in G1 and 45 patients (81.8%) in G2 showed complete resolution. Recurrence rate in G1 and G2 were 6% and 18.2%, respectively. No complications were seen in either group. In this study, clinical response at week 4 was greater in the clotrimazole group than the fluconazole group. Recurrence at week 12 after treatment was less with oral fluconazole than clotrimazole cream. So, for better evaluation, more studies need to be done.

  3. High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

    Directory of Open Access Journals (Sweden)

    Cristiane Ottoni

    2016-08-01

    Full Text Available Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc activity (80.2 U∙L−1 was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction. Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs.

  4. High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs.

    Science.gov (United States)

    Ottoni, Cristiane; Simões, Marta F; Fernandes, Sara; Santos, Cledir R; Lima, Nelson

    2016-01-01

    Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U∙L(-1)) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs. PMID:27490563

  5. Expression of phenotypic plasticity in hatchlings of the lizard Calotes versicolor (Squamata: Agamidae: influence of nest moisture

    Directory of Open Access Journals (Sweden)

    Bhagyashri A. Shanbhag

    2012-06-01

    Full Text Available Calotes versicolor breed from late May to early October. The breeding activity begins with the onset of the southwest monsoon. The eggs laid in early breeding season experience more wet conditions than those of the late breeding season. We studied the influence of nest moisture levels on the phenotypic traits of hatchlings by burying the eggs in 5-cm-deep sand nests with ~50%(wet nest or ~20% (relatively dry nest moisture to simulate nesting conditions of early and late breeding seasons. A group of eggs were subjected to standard laboratory incubation procedure in which eggs are half-buried in the moist sand and the other half exposed to air. Hatching time and hatchling body size varied with the treatment. The eggs from dry nests hatched later and hatchlings were the biggest but possessed least amount of residual yolk compared to those of 'wet nest' and also 'lab incubated' groups. In these hatchlings head and limb sizes were significantly larger than that of the other two groups. The findings show: (1 a developmental plasticity in the lizard, (2 that under low moist conditionslarger body size is preferred, and (3 that the trade-off between somatic growth of embryos and future energy reserves (residual yolk of hatchlings is influenced by the soil moisture in C. versicolor.

  6. Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolor FISCH.

    Science.gov (United States)

    Obmann, Astrid; Zehl, Martin; Purevsuren, Sodnomtseren; Narantuya, Samdan; Reznicek, Gottfried; Kletter, Christa; Glasl, Sabine

    2011-02-01

    An HPLC-diode array detection (DAD) method was established in order to investigate dried aerial parts of Dianthus versicolor FISCH. (Caryophyllaceae), a plant used in traditional Mongolian medicine against liver impairment. Aqueous extracts were separated on an Aquasil(®) C(18) column with a linear gradient of acetonitrile (ACN) and water (adjusted to pH 2.8 with formic acid) as the mobile phase. LC-IT-MS facilitated the assignment of 26 flavonoids, among them a series of rare C-glycosylated as well as O-glycosylated derivatives, which are assumed to be the active principles. Quantification was performed and validated using isovitexin-7-O-glucoside (saponarin) as the external standard. The method showed good linear behaviour (r(2) ≥0.9999) over the investigated concentration range (0.007-3.5 mg/mL). The good precision of the method allowed the successful qualitative and quantitative analysis of flavonoid-glycosides in the aqueous extracts prepared from five different D. versicolor samples. Depending on the origin of the samples, the total flavonoid content was found to vary considerably from 0.41 to 3.30% in the aqueous extracts and from 0.07 to 0.57% in the crude drug. In addition, the relative composition of the various flavonoids was found to differ strongly. These results highlight the need for proper quality control for this herbal drug. PMID:21268252

  7. Epidemiological Characters of Pityriasis Versicolor and Erythrasma among Admitted Patients of Medical Mycology Lab of Kermanshah University of Medical Sciences 1994 -2011

    Directory of Open Access Journals (Sweden)

    Mikaeili, A. (PhD

    2014-05-01

    Full Text Available Background and Objective: The rate of superficial mycoses are increasing in special conditions. This retrospective study was conducted to determine the epidemiological parameters of Pityriasis versicolor and Erythrasma during 1994- 2011. Material and Methods: In this study, we assessed the results of direct microscopic study and the other data of the patients for Pityriasis versicolor and Erythrasma in 1994-2011. Results: Of 2265, 1294 (57% suffered from pityriasis versicolor, who were mostly males (60%. The highest frequency was related to 20-29 year old and the lowest to 0-9. High school and university students were the most infected groups (33%, September was the most common period and the most common site of involvement was chest and abdomen. The people infected with Erythrasma (971; 42%, were mostly 30 -39 year old , males( 58% , urban ( 98% , reported in July and involved in groin. Conclusion: The frequency of both diseases was high in active age group, males, and the hot months of the year, which are the most common predisposing factors. Keywords: Pityriasis Versicolor; Erythrasma; Kermanshah

  8. Isolation, structure elucidation, and biomimetic total synthesis of versicolamide B and the isolation of antipodal (-)-stephacidin A and (+)-notoamide B from Aspergillus versicolor

    Science.gov (United States)

    A new prenylated indole alkaloid, versicolamide B, was isolated from cultures of Aspergillus versicolor NRRL 35600. The structure was assigned by 2D NMR data, and confirmed by a biomimetic total synthesis. Versicolamide B is the first member of the paraherquamide-stephacidin family of alkaloids fo...

  9. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor

    NARCIS (Netherlands)

    Kozarski, M.; Klaus, A.; Niksic, M.; Vrvic, M.M.; Todorovic, N.; Jakovljevic, D.; Griensven, van L.J.L.D.

    2012-01-01

    Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts wer

  10. Setting the stage for electron transfer: Molecular basis of ABTS-binding to four laccases from Trametes versicolor at variable pH and protein oxidation state

    DEFF Research Database (Denmark)

    Christensen, Niels Johan; Kepp, Kasper Planeta

    2014-01-01

    and activity for unknown reasons, and are thus useful probes of stability-function trade-offs relevant to protein engineering. We report here the first systematic comparison of ABTS-binding to different proteins, i.e. the four isoforms of Trametes versicolor, using a combination of sequence clustering, density...

  11. Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera)

    NARCIS (Netherlands)

    Alexander, K.

    2003-01-01

    Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera) Data are presented on the distribution of selected species from two coleopteran families chosen to represent a random slice of the British fauna. The species have been chosen as exhibiting extremes of range chang

  12. A coffee berry borer (Coleoptera: Curculionidae: Scolytinae) bibliography

    Science.gov (United States)

    One hundred years ago, one of the most significant biological invasions of an agricultural insect pest in the Americas was initiated. Endemic to Africa, the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae) was accidentally introduced to Brazil in 1913 and years later invaded coffe...

  13. Noteworthy records of Hispines from Belize (Coleoptera: Chrysomelidae)

    OpenAIRE

    Naczi, Robert F. C.; Charles L. Staines

    2011-01-01

    Cephaloleia consanguinea Baly, Cephaloleia fulvolimbata Baly, Cephaloleia ruficollis Baly, Chalepus amabilis Baly, Chalepus brevicornis (Baly), Chalepus pici Descarpentries and Villiers, Microrhopala erebus (Newman), Octhispa bimaculata Uhmann, Octotoma championi Baly, Pseudispa tuberculata Staines, Sceloenopla erudita (Baly), Stenispa guatemalensis Uhmann, Sumitrosis gestroi (Weise), and Sumitrosis terminatus (Baly) (Coleoptera: Chrysomelidae: Cassidinae) are new country records of hispine c...

  14. Identificação de espécies de malassésia na pitiríase versicolor em um serviço de dermatologia do sul do Brasil Identification of Malassezia yeast species isolated from patients with pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Vanessa Petry

    2011-08-01

    Full Text Available A pitiríase versicolor é uma doença de distribuição universal. Existe a descrição de 12 espécies de malassezia. O objetivo deste estudo foi determinar quais as espécies de malassezia mais prevalentes nos pacientes com pitiríase versicolor. Foram realizadas as coletas através de raspado das lesões nos pacientes com suspeita clínica de pitiríase versicolor e posterior exame micológico e cultural para identificação final da espécie. Foram coletadas amostras de 87 pacientes. Quanto às culturas, 30% foram de Malassezia sympodialis, 25,7% de Malassezia furfur, 22,7% de Malassezia globosa, 12,1% de Malassezia retrita, 7,6% de Malassezia obtusa e 1,5% de Malassezia sloofiae

  15. CLINICAL EVALUATION OF EFFICACY OF SERTACONAZOLE 2% CREAM IN TREATMENT OF PITYRIASIS VERSICOLOR AND A COMPARISION WITH THAT OF CLOTRIMAZOLE 1% CREAM

    Directory of Open Access Journals (Sweden)

    Lakshmi Tatavarthi

    2015-04-01

    Full Text Available BACKGROUND: Pityriasis versicolor is a mild superficial, chronic recurrent fungal infection. Many topical agents have been used with varying degree of success and high recurrence rate. The current study was conducted to clinically evaluate the efficacy of sertaconazole 2% in treating and preventing recurrences of pityriasis versicolor as compared to clotrimazole1%cream. AIMS: Clinical evaluation of the efficacy of sertaconazole 2% cream in the treatment of pityriasis versicolor and its comparison with that of clotrimazole 1% cream. MATERIALS & METHODS: 110 patients who were diagnosed clinically and microscopically as pityriasis versicolor and fulfilling the criteria were enrolled, of which 55 were treated with 2% sertaconazole cream and 55 with 1% clotrimazole cream twice daily for 4 weeks. At the end of fourth week patients were examined both clinically and mycologically. Follow up was done at 6 weeks to note any relapse clinically and mycologically. RESULTS : Out of 110 patients 4 patients using sertaconazole, 6 patients using clotri mazole were lost for follow - up and excluded from the study. Global assessment was done at the end of treatment i.e., 4 weeks with clinical and mycological cure rate, revealed that 42(82.3% of the sertaconazole group, 30(61.2% of the clotrimazole group we re improved clinically. Mycological examination at the same time was negative in 44(86.3%, 33(67.4% respectively. 7(13.7% of sertaconazole group, 12(24.48% of clotrimazole group show no significant change. 4(8.16% patients using clotrimazole complaine d of deterioration of symptoms but none using sertaconazole. CONCLUSION : Comparing the results obtained in this trial showed that sertaconazole was more efficacious than clotrimazole in the treatment of pityriasis versicolor because of its direct membrane damaging effect. But in terms of pigmentary resolution sertaconazole is not comparable to clotrimazole cream due to persistent effect of azelic acid.

  16. Predation by Flat Bark Beetles (Coleoptera: Silvanidae and Laemophloeidae) on Coffee Berry Borer (Coleoptera: Curculionidae) in Hawaii coffee

    Science.gov (United States)

    Coffee berry borer(CBB), Hypothenemus hampei, is a serious pest of coffee worldwide and a new invasive pest in Hawaii. Adult flat bark beetles, mainly Leptophloeus sp.(75%) and Cathartus quadricollis(21%) (Coleoptera: Laemophloeidae and Silvanidae, respectively), were found feeding in CBB-infested c...

  17. Clinical analysis of Tinea versicolor in 50 children%花斑癣50例临床分析

    Institute of Scientific and Technical Information of China (English)

    魏文

    2009-01-01

    目的 探讨儿童花斑癣的临床特征、治疗效果以及转归.方法 对50例儿童花斑癣患儿的临床资料、治疗及转归进行回顾性分析.结果 患儿面部皮损多见,夏秋季好发,乡镇儿童患病率高,发病年龄2月~14岁,平均2.3岁.男女发病率之比1.6:1.50例患儿均采用联苯苄唑治疗,有25例患儿联合特比萘芬乳膏合用治疗.50例患儿均治愈.结论 儿童花斑癣是一种少见的疾病,其皮损好发面部,与季节、环境及卫生习惯、家族易感性均密切相关.联苯苄唑是治疗儿童花斑癣首选药物,联合特比萘芬治疗有协同作用.%Objective To investigate the clinical features, therapeutic effect and prognosis of tinea versicolor in children. Methods Diagnosis, treatment and prognosis data of clinical tinea versicolor cases in 50children's were analyzed retrospectively. Results The facial skin in children had high prealence in summer, in villages and towns; children aged from 2 months to 14 years old, the average age was 2.3.The incidence rate of men and women was 1.6:1. 50 cases were treated by bifonazole cream on lesions,25 cases were treated with bifonazole cream and terbinafine cream on lesions. 50 cases of patients were cured. Conclusions Tinea versicolor in children is a rare disease, easy to occur in face, correlated with the season, environmental and health habits and family susceptibility. Bifonazole is choice for treatment of childhood tinea versieolor, terbinafine synergies is jointed treatment.

  18. Het voorkomen van de glanskevers van het genus Caprophilus in Nederland (Coleoptera: Nitidulidae)

    NARCIS (Netherlands)

    Oude, de J.

    2005-01-01

    The occurrence of nitidulid beetles of the genus Carpophilus in the Netherlands (Coleoptera: Nitidulidae) Some species of the genus Carpophilus (Coleoptera: Nitidulidae) may act as pests of stored products like cocoabeans, peanuts, dried fruits like figs and dates, imported from tropical or subtropi

  19. Secondary metabolites of a deep sea derived fungus Aspergillus versicolor CXCTD-06-6a and their bioactivity

    Science.gov (United States)

    Kong, Xianglan; Cai, Shengxin; Zhu, Tianjiao; Gu, Qianqun; Li, Dehai; Luan, Yepeng

    2014-08-01

    In order to obtain novel secondary metabolites, a deep sea inhabiting fungus Aspergillus versicolor CXCTD-06-6a was investigated. One new diketopiperazine brevianamide W ( 1a), as well as five known diketopiperazine alkaloids, diketopiperazine V ( 1b), brevianamide Q ( 2), brevianamide R ( 3), brevianamide K ( 4), and brevianamide E ( 5), were isolated from the EtOAc extract of the fermentation broth. Their structures were elucidated by spectroscopy techniques (NMR, MS). The six compounds exhibited moderate radical scavenging activity against DPPH with clearance ratio of 55.0% ( 1a and 1b), 53.7% ( 2), 46.2% ( 3), 61.4% ( 4) and 19.3% ( 5) at a concentration of 13.9 μmol L-1, respectively; while the positive control ascorbic acid showed a ratio of 70.3% at the concentration of 28.4 μmol L-1.

  20. Enzymatic removal of estrogenic activity of nonylphenol and octylphenol aqueous solutions by immobilized laccase from Trametes versicolor

    Energy Technology Data Exchange (ETDEWEB)

    Catapane, Maria [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Nicolucci, Carla; Menale, Ciro; Mita, Luigi [National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138 Naples (Italy); Rossi, Sergio [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); Mita, Damiano G., E-mail: mita@igb.cnr.it [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138 Naples (Italy); Diano, Nadia [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138 Naples (Italy)

    2013-03-15

    Highlights: ► Endocrine disruptors cause adverse effects in living organisms. ► Nonylphenol and Octylphenol are alkylphenols recognized as endocrine disruptors. ► It is necessary to remove or reduce their presence in the environment. ► Waters polluted by these pollutants have been bioremediated by immobilized laccase from Trametes versicolor. ► Laccase treated solutions were found to have lost any estrogenic activity. -- Abstract: A fluidized bed reactor, filled with laccase-based beads, has been employed to bioremediate aqueous solutions polluted by endocrine disruptors belonging to the alkylphenols (APs) class. In particular Octylphenol and Nonylphenol have been studied. The catalytic activity of free and immobilized laccase from Trametes versicolor has been characterized as a function of pH, temperature and substrate concentration in the reaction medium. In view of practical applications for each substrate concentration the removal efficiency (RE), the time to halve the initial concentration (τ{sub 50}), and the t{sub c=0}, i.e. the time to reach complete pollutant removal, have been calculated. The immobilized laccase exhibited a lower affinity for octylphenol (K{sub m} = 1.11 mM) than for Nonylphenol (K{sub m} = 0.72 mM), but all the other parameters of applicative interest resulted more significant for octylphenol. For example, the times to reach the complete removal of octylphenol compared to those for nonylphenol at the same concentration is shorter of about 15% (at low concentrations) up to 40% (at high concentrations). The study of cell proliferation with MPP89 cells, a human mesothelioma cell line, and the assay with the YES test indicated the loss of estrogenic activity of the APs solutions after laccase treatment.

  1. Historical isolation versus recent long-distance connections between Europe and Africa in bifid toadflaxes (Linaria sect. Versicolores.

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    Mario Fernández-Mazuecos

    Full Text Available BACKGROUND: Due to its complex, dynamic and well-known paleogeography, the Mediterranean region provides an ideal framework to study the colonization history of plant lineages. The genus Linaria has its diversity centre in the Mediterranean region, both in Europe and Africa. The last land connection between both continental plates occurred during the Messinian Salinity Crisis, in the late Miocene (5.96 to 5.33 Ma. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed the colonization history of Linaria sect. Versicolores (bifid toadflaxes, which includes c. 22 species distributed across the Mediterranean, including Europe and Africa. Two cpDNA regions (rpl32-trnL(UAG and trnK-matK were sequenced from 66 samples of Linaria. We conducted phylogenetic, dating, biogeographic and phylogeographic analyses to reconstruct colonization patterns in space and time. Four major clades were found: two of them exclusively contain Iberian samples, while the other two include northern African samples together with some European samples. The bifid toadflaxes have been split in African and European clades since the late Miocene, and most lineage and speciation differentiation occurred during the Pliocene and Quaternary. We have strongly inferred four events of post-Messinian colonization following long-distance dispersal from northern Africa to the Iberian Peninsula, Sicily and Greece. CONCLUSIONS/SIGNIFICANCE: The current distribution of Linaria sect. Versicolores lineages is explained by both ancient isolation between African and European populations and recent events of long-distance dispersal over sea barriers. This result provides new evidence for the biogeographic complexity of the Mediterranean region.

  2. Intoxicação experimental por Simarouba versicolor (Simaroubaceae em ovinos e indução de resistência ao consumo da planta

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    Ariany C. Santos

    2013-03-01

    Full Text Available Simarouba versicolor é uma árvore semidecídua pertencente à família Simaroubaceae. Um surto de intoxicação por S. versicolor em bovinos por brotos da planta presente no pasto em Mato Grosso do Sul e sua reprodução experimental foram descritos. Esse estudo teve por objetivos verificar experimentalmente se os ovinos podem ser utilizados como modelo clínico-patológico no estudo da intoxicação por Simarouba versicolor St. Hil. (fam. Simaroubaceae, determinar se há indução de resistência pela ingestão de pequenas e repetidas doses e, se a planta mantém sua toxicidade quando dessecada. Foram realizados dois experimentos, sendo o primeiro com folhas verdes ou folhas dessecadas e trituradas de S. versicolor em doses únicas de 5g/kg, 5g/kg e 3g/kg a três ovinos (Ovino 1, 2 e 3 respectivamente. O experimento 2, foi realizado com diferentes doses diárias de folhas dessecadas e trituradas de S. versicolor em quatro ovinos que receberam 1,5g/kg, 0,75g/kg, 0,6g/kg e 0,3g/kg e, com um ovino que recebeu 3g/kg como controle positivo (Ovino 4. A administração foi suspensa quando os ovinos apresentaram sinais clínicos da intoxicação. Após doze dias de recuperação, os animais sobreviventes foram desafiados com a mesma dose diária da planta ingerida anteriormente para avaliar o desenvolvimento de resistência. Os sinais clínicos observados nos dois experimentos caracterizaram-se por anorexia, mucosas oculares congestas, polidipsia, sialorreia, fezes pastosas que evoluíram para diarreia líquida fétida esverdeada, decúbito lateral e morte para os Ovinos 1 a 7. As principais lesões histológicas observadas foram necrose do tecido linfoide (linfonodos, baço, placas de Peyer e enterite necrosante. Com os resultados obtidos, pode-se concluir que os ovinos podem ser utilizados como modelo experimental clínico-patológico na intoxicação por S. versicolor. Com o método utilizado, não houve resistência ao consumo diário de folhas

  3. Family-Group Names In Coleoptera (Insecta

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    Patrice Bouchard

    2011-04-01

    Full Text Available We synthesize data on all known extant and fossil Coleoptera family-group names for the first time. A catalogue of 4887 family-group names (124 fossil, 4763 extant based on 4707 distinct genera in Coleoptera is given. A total of 4492 names are available, 183 of which are permanently invalid because they are based on a preoccupied or a suppressed type genus. Names are listed in a classification framework. We recognize as valid 24 superfamilies, 211 families, 541 subfamilies, 1663 tribes and 740 subtribes. For each name, the original spelling, author, year of publication, page number, correct stem and type genus are included. The original spelling and availability of each name were checked from primary literature. A list of necessary changes due to Priority and Homonymy problems, and actions taken, is given. Current usage of names was conserved, whenever possible, to promote stability of the classification. New synonymies (family-group names followed by genus-group names: Agronomina Gistel, 1848 syn. n. of Amarina Zimmermann, 1832 (Carabidae, Hylepnigalioini Gistel, 1856 syn. n. of Melandryini Leach, 1815 (Melandryidae, Polycystophoridae Gistel, 1856 syn. n. of Malachiinae Fleming, 1821 (Melyridae, Sclerasteinae Gistel, 1856 syn. n. of Ptilininae Shuckard, 1839 (Ptinidae, Phloeonomini Ádám, 2001 syn. n. of Omaliini MacLeay, 1825 (Staphylinidae, Sepedophilini Ádám, 2001 syn. n. of Tachyporini MacLeay, 1825 (Staphylinidae, Phibalini Gistel, 1856 syn. n. of Cteniopodini Solier, 1835 (Tenebrionidae; Agronoma Gistel 1848 (type species Carabus familiaris Duftschmid, 1812, designated herein syn. n. of Amara Bonelli, 1810 (Carabidae, Hylepnigalio Gistel, 1856 (type species Chrysomela caraboides Linnaeus, 1760, by monotypy syn. n. of Melandrya Fabricius, 1801 (Melandryidae, Polycystophorus Gistel, 1856 (type species Cantharis aeneus Linnaeus, 1758, designated herein syn. n. of Malachius Fabricius, 1775 (Melyridae, Sclerastes Gistel, 1856 (type species

  4. A checklist of stag beetles (Coleoptera: Scarabaeoidea: Lucanidae) from Iran.

    Science.gov (United States)

    Bartolozzi, Luca; Ghahari, Hassan; Sprecher-Uebersax, Eva; Zilioli, Michele

    2014-11-26

    An updated checklist of the Lucanidae (Coleoptera) from Iran is given. New locality records are listed and some dubious distributional records are discussed. Dorcus vavrai Nonfried, 1905 is placed in synonymy with Dorcus peyronis Reiche and Saulcy, 1856 (new synonymy) The female of Lucanus xerxes Král, 2004 is described. A key for the identification of the Iranian stag beetle species is also provided and all the species are figured.

  5. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, Jessica; Verheggen, François; Lognay, Georges; Haubruge, Eric

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and...

  6. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, J.; Verheggen, F.; Lognay, G.; Haubruge, E.

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and ...

  7. Cell-mediated immune responses to Malassezia furfur serovars A, B and C in patients with pityriasis versicolor, seborrheic dermatitis and controls.

    Science.gov (United States)

    Ashbee, H R; Ingham, E; Holland, K T; Cunliffe, W J

    1994-06-01

    It has been postulated that patients with Malassezia furfur-associated dermatoses have a deficient cell-mediated immune response to M. furfur. This study examined the cell-mediated immune responses to M. furfur serovars A, B and C of 10 patients with pityriasis versicolor and 10 age- and sex-matched controls; and 10 patients with seborrheic dermatitis and 10 age- and sex-matched controls. The responses to each serovar of M. furfur were assessed using the lymphocyte transformation assay and the leukocyte migration inhibition assay. The lymphocyte transformation responses of the patients with pityriasis versicolor to M. furfur serovars A, B and C (0/10, 6/10 and 5/10 respectively) were not significantly different from those of controls (0/10, 2/10 and 1/10). However, for patients with seborrheic dermatitis, significantly more patients' lymphocytes responded to serovars B and C (6/10 and 6/10 respectively) than those of controls (1/10 and 1/10). No patient or control responded to serovar A. In the leukocyte migration inhibition assay, the leukocytes from a greater proportion of patients with pityriasis versicolor (5/7) responded to serovar B than controls (2/10); and the leukocytes from a greater proportion of patients with seborrheic dermatitis (4/10) responded to serovar C than controls (0/9). Thus, this data did not indicate the presence of any cell-mediated immune deficiency to M. furfur in patients with pityriasis versicolor or seborrheic dermatitis, as measured by the lymphocyte transformation assay or the leukocyte migration inhibition assay. The greater responsiveness of T lymphocytes from patients may indicate that T lymphocytes might be involved in the pathogenesis of these diseases.

  8. A novel contrast stain for the rapid diagnosis of pityriasis versicolor: A comparison of Chicago Sky Blue 6B stain, potassium hydroxide mount and culture

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    Nikita Lodha

    2015-01-01

    Full Text Available Background: The mycological study of pityriasis versicolor is usually done by potassium hydroxide (KOH mount and culture. However, KOH mount lacks a color contrast and requires a trained eye to interpret, while culture is difficult to perform, time consuming and has low sensitivity. Chicago Sky Blue 6B (CSB is a new contrast stain that highlights the fungal hyphae and spores, blue against a purplish background. Aims and Objectives: This study was done to compare the utility of a novel contrast stain (CSB stain with KOH mount and culture. Materials and Methods: Skin scrapings from the lesions of 100 clinically diagnosed cases of P. versicolor were subjected to (1 KOH mount and CSB stain for direct microscopic examination and (2 culture using Sabouraud′s dextrose agar. The statistical analysis of CSB stain and culture was done using KOH mount as the reference method, as it is the most commonly performed and practical diagnostic test available for P. versicolor. An interrater reliability analysis using the Cohen′s Kappa statistic was performed to determine consistency (agreement among the different modalities. Observations and Results: Direct microscopy with CSB stain, KOH mount and mycological culture showed positive results in 98 (98%, 92 (92% and 56 (56% patients, respectively. Using KOH mount as the reference method, CSB stain had a sensitivity of 100% which was significantly higher than culture (60.9%. Statistically significant fair agreement was found between CSB stain and KOH mount (94% with κ=0.38, P < 0.001. Negligible agreement was found between CSB stain and culture (66%, κ=0.199, P = 0.001 as well as between KOH mount and culture (64%, κ=0.051, P = 0.107. Conclusion: CSB staining of skin scrapings is the most sensitive method for the diagnosis of pityriasis versicolor. Due to the distinct contrast provided by CSB, it is easy to perform, rapid and qualitatively superior to KOH mount.

  9. Nocturnal Migration of Coleoptera: Carabidae in North China

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Vertical-looking radar (VLR) has allowed long-term automatic monitoring of the altitudinal and temporal dynamics of high-flying insect populations. To investigate whether ground beetle, insect of Coleoptera, was capable of migrating and its migration pattern by taking advantages of capability of the VLR for long-term real-time automatic monitoring, the migration of Coleoptera ground beetle was investigated by setting up radar observation points, making long-term observation using the VLR and related supplementary equipment, and analyzing low altitude air current and large area circulating current in combination with the meteorological data. Information obtained in 2005 and 2006 showed that the seasonal activities of ground beetles traps of trap lamps were mainly from late June to late August, peak period was mainly in August, seasonal traps of high-altitude lamps and ground lamps were featured by sudden increase and sudden decrease; in peak period, the height of radar echo point could be as high as 600 m, while it was mainly below the height of 450 m; night activities mainly occurred from 20:00 to 22:00, in very few nights, radar echo could last until about 04:00, changes in numbers of ground beetles within the searchlights were consistent with radar echo intensity; ground beetle images were successfully trapped in the sweep nets carried by captive balloons at the height of 200 m. Some species of Carabidaes had some degrees of migration, thus providing the foundation for investigating the migration of Coleoptera insects.

  10. Estados inmaduros de Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini Immature stages of Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini

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    Jhon César Neita-Moreno

    2008-12-01

    Full Text Available Se describen y se ilustran por primera vez la larva de tercer estadio y la pupa de Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini. Se aportan datos sobre la biología de la especie y su distribución en Colombia. Se proporciona una clave para la identificación de las larvas de tercer estadio conocidas de las especies del género Ancognatha Erichson.The third instar larva and pupa of Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini are described and illustrated for the first time. New data on larval biology and distribution of the species in Colombia are included. A key to the known third stage larvae of Ancognatha Erichson is provided.

  11. Enhanced oxidation of benzo[a]pyrene by crude enzyme extracts produced during interspecific fungal interaction of Trametes versicolor and Phanerochaete chrysosporium

    Institute of Scientific and Technical Information of China (English)

    Linbo Qian; Baoliang Chen

    2012-01-01

    The effects of interspecific fungal interactions between Trametes versicolor and Phanerochaete chrysosporium on laccase activity and enzymatic oxidation of polycyclic aromatic hydrocarbons (PAHs) were investigated.A deadlock between the two mycelia rather than replacement of one fungus by another was observed on an agar medium.The laccase activity in crude enzyme extracts from interaction zones reached a maximum after a 5-day incubation,which was significantly higher than that from regions of T.versicolor or P.chrysosporium alone.The enhanced induction of laccase activity lasted longer in half nutrition than in normal nutrition.A higher potential to oxidize benzo[a]pyrene by a crude enzyme preparation extracted from the interaction zones was demonstrated.After a 48 hr incubation period,the oxidation of benzo[a]pyrene by crude enzyme extracts from interaction zones reached 26.2%,while only 9.5% of benzo[a]pyrene was oxidized by crude extracts from T.versicolor.The oxidation was promoted by the co-oxidant 2,2'-azinobis-3-ethylbenzthiazoline-6-sulphonate diammonium salt (ABTS).These findings indicate that the application of co-culturing of white-rot fungi in bioremediation is a potential ameliorating technique for the restoration of PAH-contaminated soil.

  12. Biodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC-QqTOF-MS

    Energy Technology Data Exchange (ETDEWEB)

    Garcia-Galan, Ma. Jesus, E-mail: mggqam@cid.csic.es [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona (Spain); Rodriguez-Rodriguez, Carlos E., E-mail: CarlosEsteban.Rodriguez@uab.cat [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 2060 San Jose (Costa Rica); Vicent, Teresa, E-mail: Teresa.Vicent@uab.cat [Departament d' Enginyeria Quimica, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Caminal, Gloria, E-mail: Gloria.Caminal@uab.cat [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Diaz-Cruz, M. Silvia, E-mail: sdcqam@cid.csic.es [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona (Spain); Barcelo, Damia, E-mail: dbcqam@cid.csic.es [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona (Spain); Catalan Institute for Water Research (ICRA), Parc Cientific i Tecnologic de la Universitat de Girona. C/Emili Grahit, 101 Edifici H2O, E-17003 Girona (Spain); King Saud University, P.O. Box 2455, Riyadh 11451 (Saudi Arabia)

    2011-11-15

    Degradation of the sulfonamide sulfamethazine (SMZ) by the white-rot fungus Trametes versicolor was assessed. Elimination was achieved to nearly undetectable levels after 20 h in liquid medium when SMZ was added at 9 mg L{sup -1}. Experiments with purified laccase and laccase-mediators resulted in almost complete removal. On the other hand, inhibition of SMZ degradation was observed when piperonilbutoxide, a cytochrome P450-inhibitor, was added to the fungal cultures. UPLC-QqTOF-MS analysis allowed the identification and confirmation of 4 different SMZ degradation intermediates produced by fungal cultures or purified laccase: desulfo-SMZ, N{sup 4}-formyl-SMZ, N{sup 4}-hydroxy-SMZ and desamino-SMZ; nonetheless SMZ mineralization was not demonstrated with the isotopically labeled sulfamethazine-phenyl-{sup 13}C{sub 6} after 7 days. Inoculation of T. versicolor to sterilized sewage sludge in solid-phase systems showed complete elimination of SMZ and also of other sulfonamides (sulfapyridine, sulfathiazole) at real environmental concentrations, making this fungus an interesting candidate for further remediation research. - Highlights: {yields}Degradation of sulfamethazine by Trametes versicolor was evaluated. {yields}The laccase enzymatic system and cytochrome P-450 were involved in the degradation. {yields}Four different degradation products of sulfamethazine were identified and confirmed. {yields}The molecular structures and masses of the metabolites were accurately calculated. {yields}Full elimination of sulfamethazine was observed in regular sewage sludge.

  13. The effect of citrate and sulfate of copper on the biomass composition of the medicinal mushroom Trametes versicolor (Polyporales, Polyporaceae

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    G. A. Al-Maali

    2016-03-01

    Full Text Available The aim of our research was to study the influence of citrate and sulfate of copper on the biomass composition of the mycelium of the medicinal mushroom Trametes versicolor (L. Lloyd, (1920 cultivated in a liquid medium. The studied strain of Trametes versicolor 353 was obtained from the Culture Collection of Mushrooms (IBK from M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine. Copper citrate was obtained from the Institute of Nanobiotechnologies and Resource Conservation of Ukraine, Kyiv. In this study we used glucose-peptone-yeast extract medium. Cu2+ (sulfate or citrate form was added to the medium in concentration 4 mg/L. Mycelium was grown in a submerged culture on a rotary shaker (120 rpm at 26 °C in 250 ml Erlenmeyer flasks, containing 50 ml of liquid media. The biomass was harvested after 9 days of cultivation in the liquid medium, filtered, washed, dried to a constant weight at 105 °C and weighed. Total nitrogen content (Ntotal in the mycelium determined by the Kjeldahl method, crude protein content was determined as Ntotal x 6.25. The ash was obtained by the standard method. Total lipids were extracted from undried mycelium by a modified method of Bligh and Dyer. Amino acid composition was analyzed by high-performance liquid chromatography Agilent 1200 (Agilent technologies, USA. The methyl ethers of fatty acids were determined by gas chromatography-mass spectrometry (GC/MS Agilent 6890N/5973 inert. The results of our research demonstrated that sulfates and citrates of copper increased the amount of crude protein on the mycelium of T. versicolor 353. Also both form of copper increased the amount of ash by a third relative to the control medium. At the same time, both forms of copper reduced the amount of total carbohydrates on mycelial biomass. But copper sulfate reduced the amount of total lipids relative to the control medium and medium with copper citrate. It should be noted, that given the significant

  14. Activation of Dormant Secondary Metabolite Production by Introducing Neomycin Resistance into the Deep-Sea Fungus, Aspergillus versicolor ZBY-3

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    Yuan Dong

    2014-07-01

    Full Text Available A new ultrasound-mediated approach has been developed to introduce neomycin-resistance to activate silent pathways for secondary metabolite production in a bio-inactive, deep-sea fungus, Aspergillus versicolor ZBY-3. Upon treatment of the ZBY-3 spores with a high concentration of neomycin by proper ultrasound irradiation, a total of 30 mutants were obtained by single colony isolation. The acquired resistance of the mutants to neomycin was confirmed by a resistance test. In contrast to the ZBY-3 strain, the EtOAc extracts of 22 of the 30 mutants inhibited the human cancer K562 cells, indicating that these mutants acquired a capability to produce antitumor metabolites. HPLC-photodiode array detector (PDAD-UV and HPLC-electron spray ionization (ESI-MS analyses of the EtOAc extracts of seven bioactive mutants and the ZBY-3 strain indicated that diverse secondary metabolites have been newly produced in the mutant extracts in contrast to the ZBY-3 extract. The followed isolation and characterization demonstrated that six metabolites, cyclo(d-Pro-d-Phe (1, cyclo(d-Tyr-d-Pro (2, phenethyl 5-oxo-l-prolinate (3, cyclo(l-Ile-l-Pro (4, cyclo(l-Leu-l-Pro (5 and 3β,5α,9α-trihydroxy-(22E,24R-ergosta-7,22-dien-6-one (6, were newly produced by the mutant u2n2h3-3 compared to the parent ZBY-3 strain. Compound 3 was a new compound; 2 was isolated from a natural source for the first time, and all of these compounds were also not yet found in the metabolites of other A. versicolor strains. Compounds 1–6 inhibited the K562 cells, with inhibition rates of 54.6% (1, 72.9% (2, 23.5% (3, 29.6% (4, 30.9% (5 and 51.1% (6 at 100 μg/mL, and inhibited also other human cancer HL-60, BGC-823 and HeLa cells, to some extent. The present study demonstrated the effectiveness of the ultrasound-mediated approach to activate silent metabolite production in fungi by introducing acquired resistance to aminoglycosides and its potential for discovering new compounds from silent

  15. Activation of dormant secondary metabolite production by introducing neomycin resistance into the deep-sea fungus, Aspergillus versicolor ZBY-3.

    Science.gov (United States)

    Dong, Yuan; Cui, Cheng-Bin; Li, Chang-Wei; Hua, Wei; Wu, Chang-Jing; Zhu, Tian-Jiao; Gu, Qian-Qun

    2014-07-29

    A new ultrasound-mediated approach has been developed to introduce neomycin-resistance to activate silent pathways for secondary metabolite production in a bio-inactive, deep-sea fungus, Aspergillus versicolor ZBY-3. Upon treatment of the ZBY-3 spores with a high concentration of neomycin by proper ultrasound irradiation, a total of 30 mutants were obtained by single colony isolation. The acquired resistance of the mutants to neomycin was confirmed by a resistance test. In contrast to the ZBY-3 strain, the EtOAc extracts of 22 of the 30 mutants inhibited the human cancer K562 cells, indicating that these mutants acquired a capability to produce antitumor metabolites. HPLC-photodiode array detector (PDAD)-UV and HPLC-electron spray ionization (ESI)-MS analyses of the EtOAc extracts of seven bioactive mutants and the ZBY-3 strain indicated that diverse secondary metabolites have been newly produced in the mutant extracts in contrast to the ZBY-3 extract. The followed isolation and characterization demonstrated that six metabolites, cyclo(D-Pro-D-Phe) (1), cyclo(D-Tyr-D-Pro) (2), phenethyl 5-oxo-L-prolinate (3), cyclo(L-Ile-L-Pro) (4), cyclo(L-Leu-L-Pro) (5) and 3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (6), were newly produced by the mutant u2n2h3-3 compared to the parent ZBY-3 strain. Compound 3 was a new compound; 2 was isolated from a natural source for the first time, and all of these compounds were also not yet found in the metabolites of other A. versicolor strains. Compounds 1-6 inhibited the K562 cells, with inhibition rates of 54.6% (1), 72.9% (2), 23.5% (3), 29.6% (4), 30.9% (5) and 51.1% (6) at 100 μg/mL, and inhibited also other human cancer HL-60, BGC-823 and HeLa cells, to some extent. The present study demonstrated the effectiveness of the ultrasound-mediated approach to activate silent metabolite production in fungi by introducing acquired resistance to aminoglycosides and its potential for discovering new compounds from silent fungal

  16. A new species of the genus Falsoibidion Pic (Coleoptera, Cerambycidae) from Korea

    OpenAIRE

    Lee, Seunghyun; Lee, Seunghwan

    2016-01-01

    A new species of the genus Falsoibidion Pic, 1922 (Coleoptera, Cerambycidae, Cerambycinae, Callidiopini) from Korea is described. Habitus and genitalia of male and female of the new species are illustrated.

  17. A new species of the genus Falsoibidion Pic (Coleoptera, Cerambycidae) from Korea

    Science.gov (United States)

    Lee, Seunghyun; Lee, Seunghwan

    2016-01-01

    Abstract A new species of the genus Falsoibidion Pic, 1922 (Coleoptera, Cerambycidae, Cerambycinae, Callidiopini) from Korea is described. Habitus and genitalia of male and female of the new species are illustrated. PMID:27563272

  18. Parasitylenchus sp. (Tylenchomorpha: Allantonematidae) parasitizing field populations of Harmonia axyridis (Coleoptera: Coccinellidae)

    DEFF Research Database (Denmark)

    Harding, Susanne; Poinar, George O. Jr.; Dimitrova, Desislava V.;

    2011-01-01

    Adults of the invasive harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae), were found to be parasitized by nematodes (Tylenchomorpha: Allantonematidae) in Denmark. The nematodes were identified as Parasitylenchus sp. Major morphological characters of the nematodes did not differ...

  19. Significantly higher Carabid beetle (Coleoptera: Carabidae) catch in conventionally than in organically managed Christmas tree plantations

    DEFF Research Database (Denmark)

    Bagge, Søren; Lund, Malthe; Rønn, Regin;

    2012-01-01

    trapped carabid beetles (Coleoptera, Carabidae) varied between conventionally and organically managed Caucasian Fir (Abies nordmanniana (Stev.)) plantations, in northern Zealand, Denmark. We recorded significantly higher numbers of carabid beetle specimens and species at conventionally than at organically...

  20. Apostasimerini (Coleoptera: Curculionidae: Baridinae). Rectification of authorship, year of publication, rank, and taxa included

    Science.gov (United States)

    The following nomenclatural changes are proposed in the Coleoptera, Curculionidae: the author of Apostasimerini is Schoenherr (1844), not Lacordaire (1866); Madopterini Lacordaire, 1866 is demoted to subtribe of Apostasimerini; Erirhinus mourei Bondar, 1943 is a new synonym of Apostasimerus serriros...

  1. First record of Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae in Brazil

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2015-03-01

    Full Text Available Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae is recorded for the first time in Brazil (Bahia. It was originally described from Europe and is currently widely distributed in that continent and Asia.

  2. Primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae and Disteniidae) of the Smithsonian Institution

    Science.gov (United States)

    The primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae, Disteniidae) of the National Museum of Natural History (Smithsonian Institution) are catalogued and figured, current through 2012 (but also including some 2013 holotypes). Data on the original combination, current combina...

  3. Methods for assessing infestations of sunflower stem weevil (Coleoptera: Curculionidae) in sunflower stems

    Science.gov (United States)

    The sunflower stem weevil, Cylindrocopturus adspersus LeConte (Coleoptera: Curculionidae), reduces sunflower, Helianthus annuus L. (Asteraceae), yields by spreading pathogens, damaging vascular tissues, and promoting lodging of sunflower plants. To assess weevil populations for host plant resistanc...

  4. A new species of Scymnobius Casey (Coleoptera, Coccinellidae, Scymnini from Pernambuco, Brazil

    Directory of Open Access Journals (Sweden)

    José Adriano Giorgi

    2014-12-01

    Full Text Available A new species of Scymnobius Casey (Coleoptera, Coccinellidae, Scymnini from Pernambuco, Brazil. Scymnobius pernambucensis sp. nov. from Pernambuco, Brazil, is described and illustrated. This is the third species of this genus recorded from Brazil.

  5. Pomegranate peels: A novel ago-residue for tannate production by gamma irradiated aspergillus versicolor under solid state fermentation

    International Nuclear Information System (INIS)

    Pomegranate peels were tested as a substrate for the production of tannate and gallic acid (GA) under solid state fermentation (SSF) using Aspergillus versicolor-g3B (highly potent gamma irradiated tannate producer isolate). The fungal strain was grown on the substrate without any pre-treatment. Different parameters for maximum production of tannate by this strain under SSF method were optimized. The maximum tannate yield (12.45μg-1ds) and GA (94%) were obtained when SSF was carried out at ph 5.0, 30 degree C, 60% initial substrate moisture, 20% inoculum size, 3% tannic acid and 1% ammonium nitrate as additional carbon and nitrogen sources, after 96 h of fermentation. The crude tannate from this isolate showed ph and temperature optima of 5.0 and 50 degree C, respectively. Also, the enzyme was stable in a broad ph range of 3-8 for 24 h and was also heat stable as it retained 65% activity at 70 degree C after 1 hour

  6. DECOLORIZATION OF TEXTILE DYES BY NEWLY ISOLATED TRAMETES VERSICOLOR STRAIN Sevil PİLATİN, Buket KUNDUHOĞLU

    Directory of Open Access Journals (Sweden)

    Buket KUNDUHOĞLU

    2011-08-01

    Full Text Available In this century, the amount of industrial produces and their consumption has increased tremendously. Along with this increase, accumulation of industrial waste and its effect on nature has causedserious problems. Because of including various chemicals and especially dye, textile waste water is one of the most hazardous industrial wastes. Color is the most important pollutant in waste water and it should be decolorized. Decolorization is more important than degradation of organic substances from waste water. Even a small amount of dye found changes the color of rivers, lakes and other waterresources, and reduces the penetration of light and solubility of gases. White rot fungus are used as a biological system in degradation and decolorizing of textile dyes.In this study, parameters for decolorization of several textile dyes (Blue 49, Orange 12, Orange 13, Red 31, Black 5, RBBR by newly isolated Trametes versicolor M96was studied.It has been determined that pH, amount of inoculum, shaking speed (rpm, dye concentration and temperature are important factors in decolorization of the studied dyes. The maximum decolorization was found pH 4.5, amount of inoculums 50 ml, shaking speed 200 rpm, dye concentration 50 mg/l and heat 30 °C.

  7. Liquefaction of coal by Polyporus versicolor and Poria monticola. Final report, 1 September 1984-31 August 1986

    Energy Technology Data Exchange (ETDEWEB)

    Cohen, M.S.

    1986-01-01

    Polyporus versicolor (ATCC 12679), obtained from the American Type Culture Collection, Rockville, MD, has been demonstrated to degrade leonardite, lignite, and subbituminous coals to a black liquid product which is called the bioextract. The process of solubilizing the coal has been termed liquification. Fungi were routinely maintained in both solid Sabouraud maltose agar (6%) and in Sabouraud maltose broth cultures. All cultures were incubated at 30/sup 0/C, 84 to 98% relative humidity, and pH = 5.8. All materials which came into contact with the fungi were sterilized before use. Experimental cultures were incubated as described for stock cultures. Cultures were incubated for approximately 12 days to produce a mature fungal mat across a glass petri dish. Coal pieces (approximately 5 mm/sup 3/) were placed directly on the hyphal mat. Liquified coal (the bioextract) was removed from the top of the mycelium and/or coal pieces and either stored for analyses at 4/sup 0/C or else freeze-dried and stored dessicated at room temperature. The bioextract has been produced in sufficient quantity to permit various methods of analysis including high performance liquid chromatography, UV-visible spectrophotometry, titrimetry, electrophoresis, proton nmr spectroscopy, and calorimetry. The solubility of the bioextract in different solvents has also been determined. 6 refs., 26 figs., 3 tabs.

  8. An analysis of the Malassezia species distribution in the skin of patients with pityriasis versicolor in Chengdu, China.

    Science.gov (United States)

    Xie, Zhen; Ran, Yuping; Zhang, Hao; Zhang, Min; Wan, Huiying; Li, Conghui

    2014-01-01

    Pityriasis versicolor (PV) is a common clinical problem associated with Malassezia species (Malassezia spp.). Controversies remain regarding the specific species involved in the development of PV. This study analyzed the difference in Malassezia spp. distribution in lesional and nonlesional skin in Chinese PV patients. A paired design was applied. Lesional and nonlesional scales from 24 cases were collected; real-time fluorescence quantitative PCR was used to detect 10 different Malassezia spp. In lesional skin, the highest detection rates were for M. globosa (95.8%), M. restricta (91.7%), and M. sympodialis (50.0%). In nonlesional skin, the highest detection rates were for M. globosa (87.5%), M. restricta (79.2%), and M. dermatis (33.3%). A significant difference in the detection rate was only found for M. sympodialis (50.8% versus 20.8%, P = 0.04). Compared with nonlesional skin, the amount of M. globosa, M. restricta, and M. sympodialis in lesional skin was significantly higher (3.8 ± 1.3,  2.5 ± 1.1, and 3.2 ± 1.6 times higher, resp.). The results of this study do not indicate that M. globosa and M. restricta are directly correlated with PV development; however, M. sympodialis is more likely related to PV development in Chinese individuals.

  9. Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tertiary Care Hospital, Punjab

    Directory of Open Access Journals (Sweden)

    M Kaur

    2013-01-01

    Full Text Available Purpose: Pityriasis versicolor (PV is a chronic superficial fungal disease caused by Malassezia species. Our aim was to identify Malassezia species from PV patients and healthy individuals in Punjab. Materials and Methods: Modified Dixon agar was used as isolation culture medium. Identification was based on morphological observation and biochemical evaluation. The biochemical evaluation consisted of culture onto Sabouraud dextrose agar, catalase reaction, Tween assimilation, Cremophor EL assimilation, splitting of esculin and growth at 38 0 C. Results: Out of 58 microscopically diagnosed cases of PV, growth was obtained from 54 (93.10% cases. The most frequently isolated species were M. globosa, M. sympodialis and M. furfur which made up 51.79%, 31.42% and 18.51% of the isolated etiological agents respectively. However, the major isolate from the back of healthy individuals was M. sympodialis (47.61%, followed by M.obtusa (19.04%, M. globosa (14.20%, M. furfur (9.52%, M. pachydermatis (4.76% and M. slooffiae (4.76%. Conclusions: M. globosa in its mycelial phase was the main etiological agent, but as normal flora from the back of healthy subjects, it was found in significantly less number (P = 0.01, suggesting that the higher pathogenicity of M. globosa in terms of enzymatic endowment, might be the cause of its predominance in PV lesions.

  10. An Analysis of the Malassezia Species Distribution in the Skin of Patients with Pityriasis Versicolor in Chengdu, China

    Directory of Open Access Journals (Sweden)

    Zhen Xie

    2014-01-01

    Full Text Available Pityriasis versicolor (PV is a common clinical problem associated with Malassezia species (Malassezia spp.. Controversies remain regarding the specific species involved in the development of PV. This study analyzed the difference in Malassezia spp. distribution in lesional and nonlesional skin in Chinese PV patients. A paired design was applied. Lesional and nonlesional scales from 24 cases were collected; real-time fluorescence quantitative PCR was used to detect 10 different Malassezia spp. In lesional skin, the highest detection rates were for M. globosa (95.8%, M. restricta (91.7%, and M. sympodialis (50.0%. In nonlesional skin, the highest detection rates were for M. globosa (87.5%, M. restricta (79.2%, and M. dermatis (33.3%. A significant difference in the detection rate was only found for M. sympodialis (50.8% versus 20.8%, P=0.04. Compared with nonlesional skin, the amount of M. globosa, M. restricta, and M. sympodialis in lesional skin was significantly higher (3.8±1.3,   2.5±1.1, and 3.2±1.6 times higher, resp.. The results of this study do not indicate that M. globosa and M. restricta are directly correlated with PV development; however, M. sympodialis is more likely related to PV development in Chinese individuals.

  11. Extracellular polysaccharide with novel structure and antioxidant property produced by the deep-sea fungus Aspergillus versicolor N2bc.

    Science.gov (United States)

    Yan, Meng-Xia; Mao, Wen-Jun; Liu, Xue; Wang, Shu-Yao; Xia, Zheng; Cao, Su-Jian; Li, Jing; Qin, Ling; Xian, Hua-Li

    2016-08-20

    An extracellular polysaccharide, N1, was obtained from the culture medium of the deep-sea fungus Aspergillus versicolor N2bc by a combination of ethanol precipitation, ion-exchange and gel filtration chromatography. N1 was a mannoglucogalactan with molecular weight of about 20.5kDa. Results of chemical and spectroscopic analyses, including Fourier-transform infrared, one- and two-dimensional nuclear magnetic resonance spectroscopy showed that the main chain of N1 consisted of →2)-α-d-Glcp-(1→, →2)-β-d-Glcp-(1→ and →6)-β-d-Manp-(1→ units, substituted at C-6 position of →2)-α-d-Glcp-(1→ units. The branches were composed of galactofuranose-oligosaccharides built up of →5)-β-d-Galf-(1→, →6)-β-d-Galf-(1→ and terminal β-d-Galf units. At an average, there were two branching points for every five sugar residues in the backbone. N1 possessed a high in vitro antioxidant activity as evaluated by scavenging assays involving superoxide, 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radicals and reducing power. The investigation revealed that N1 was a novel antioxidant polysaccharide differing from previously described extracellular polysaccharides and could be a potential antioxidant. PMID:27178933

  12. Desulphurization of some low-rank Turkish lignites with crude laccase produced from Trametes versicolor ATCC 200801

    Energy Technology Data Exchange (ETDEWEB)

    Aytar, Pinar; Gedikli, Serap [Graduate School of Natural and Applied Sciences, Eskisehir Osmangazi University (Turkey); Sam, Mesut [Department of Biology, Faculty of Arts and Science, Aksaray University (Turkey); Uenal, Arzu [Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Research, Ankara (Turkey); Cabuk, Ahmet [Department of Biology, Faculty of Arts and Science, Eskisehir Osmangazi University (Turkey); Kolankaya, Nazif [Department of Biology, Division of Biotechnology, Faculty of Science, Hacettepe University, Ankara (Turkey); Yueruem, Alp [Grand Water Research Institute, Technion Israel Institute of Technology, Haifa (Israel)

    2011-01-15

    In this paper, data obtained during the oxidative desulphurization of some low-rank Turkish lignites with crude laccase enzyme produced from Trametes versicolor ATCC 200801 are presented. In order to optimize desulphurization conditions, effects of incubation time, pulp density, incubation temperature, medium pH, and also lignite source on the desulphurization have been examined. The values for incubation period, pulp density, temperature and pH in optimum incubation condition were found as 30 min, 5%, 35 C, and pH 5.0, respectively. Under optimum conditions, treatment of coal samples with crude laccase has caused nearly 29% reduction in their total sulphur content. During the study, the rate of desulphurization of coal sample provided from Tuncbilek with crude laccase was found to be relatively higher than the other examined coal samples. Results of analytical assays have indicated that the treatment of coals with crude laccase has caused no change in their calorific values but reduced their sulphur emissions. 35%, 13%, and 25% reductions of pyritic sulphur, sulphate and organic sulphur in a period of 30 min were achieved, for a particle size of 200 {mu}m under optimal conditions with enzymatic desulphurization. Also, statistical analyses such as Tukey Multiple Comparison tests and ANOVA were performed. (author)

  13. Aspectos clínicos de pacientes com pitiríase versicolor atendidos em um centro de referência em dermatologia tropical na cidade de Manaus (AM, Brasil Clinical aspects of patients with pityriasis versicolor seen at a referral center for tropical dermatology in Manaus, Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Patrícia Motta de Morais

    2010-12-01

    Full Text Available FUNDAMENTOS: A pitiríase versicolor (tinha versicolor é uma micose superficial crônica, causada por leveduras do gênero Malassezia spp. comensais das camadas queratinizadas da pele e que, sob determinadas condições ainda não esclarecidas, se torna patogênica, determinando as manifestações clínicas da doença. É uma dermatose recidivante e, mesmo após tratamento, pode deixar hipopigmentação persistente, causando problemas sociais aos indivíduos acometidos. OBJETIVO: Descrever as características clínicas e epidemiológicas de pacientes com diagnóstico de tinha versicolor atendidos em uma unidade de referência em Dermatologia (Fundação Alfredo da Matta. MÉTODOS: Estudo de série de casos em que foram detalhadas as manifestações cutâneas e as características epidemiológicas de pacientes atendidos na Fundação Alfredo da Matta com diagnóstico de tinha versicolor. RESULTADOS: Cento e dezesseis pacientes foram incluídos no estudo no período de janeiro a agosto de 2008. A maioria dos indivíduos é do sexo masculino, de cor parda, da faixa etária jovem e formada por estudantes, que apresentavam fatores predisponentes ao surgimento das manchas. Também a maioria apresentava lesões extensas e história passada da doença. CONCLUSÃO: O estudo mostrou alta proporção de indivíduos com quadros extensos e de longa duração da doença.BACKGROUND: Pityriasis versicolor (tinea versicolor is a chronic superficial mycosis caused by yeasts of the Malassezia spp. genus commensal of the keratinized layers of the skin. Under conditions not yet understood, it becomes pathogenic determining the clinical manifestations of the disease. It is a recurrent skin condition and persistent hypopigmentation may remain after treatment, causing social problems to those affected. OBJECTIVE: To describe the clinical and epidemiological features of patients diagnosed with tinea versicolor treated at a referral center for dermatology (Alfredo da

  14. Revision of the genus Altitatiayus Weinreich(Coleoptera, Lucanidae, Lucaninae

    Directory of Open Access Journals (Sweden)

    Paschoal C. Grossi

    2010-01-01

    Full Text Available Revision of the genus Altitatiayus Weinreich (Coleoptera, Lucanidae, Lucaninae. The South American genus Altitatiayus Weinreich is revised and now includes six species, A. rotundatus (Boileau, A. ruficollis (Lüderwaldt, A. godinhorum (Bomans & Arnaud, A. dulceae (Bomans & Arnaud, A. trifurcatus (Grossi & Racca-Filho and A. koikei sp. nov. (Minas Gerais, Brazil. All species are described and illustrated. For the first time male and female genitalia are illustrated for five species and observations on the behavior of two species are included.

  15. Papel dos besouros (Insecta, Coleoptera) na Entomologia Forense

    OpenAIRE

    Wellington Emanuel dos Santos

    2014-01-01

    Esse trabalho apresenta uma revisão do papel que os besouros (Insecta, Coleoptera) desempenham na Entomologia Forense. Discussões sobre ocorrência em cadáveres humanos e carcaças animais, estimativas de Intervalo Pós-Morte (IPM), estudos realizados no Brasil e em outros países, principais famílias de importância forense e aspectos biológicos, ecológicos e biogeográficos das espécies são apresentadas.

  16. Fossil history of Mesozoic weevils (Coleoptera:Curculionoidea)

    Institute of Scientific and Technical Information of China (English)

    Andrei A.Legalov

    2012-01-01

    The first synopsis of Mesozoic weevils (Curculionoidea: Coleoptera) is presented.Changes of family,genera and species abundance during the Mesozoic revealed three distributional patterns.The Jurassic (Karatau) fauna was dominated by the Nemonychidae.During the Early Cretaceous (beginning at the Jurassic/Cretaceous border),the Ithyceridae was the prevalent group with a significant role played by the Nemonychidae.In the Late Cretaceous (Cenomanian and Turonian),the major groups were the Curculionidae and Brentidae.Obviously,the change of weevil fauna during this period was due to the expansion of the angiosperms,which provided multiple niches in their vegetative and reproductive organs for weevil development.

  17. Intoxicação experimental por Simarouba versicolor (Simaroubaceae em ovinos e indução de resistência ao consumo da planta Experimental poisoning by Simarouba versicolor (Simaroubaceae in sheep and induced resistance to consumption of the plant

    Directory of Open Access Journals (Sweden)

    Ariany C. Santos

    2013-03-01

    Full Text Available Simarouba versicolor é uma árvore semidecídua pertencente à família Simaroubaceae. Um surto de intoxicação por S. versicolor em bovinos por brotos da planta presente no pasto em Mato Grosso do Sul e sua reprodução experimental foram descritos. Esse estudo teve por objetivos verificar experimentalmente se os ovinos podem ser utilizados como modelo clínico-patológico no estudo da intoxicação por Simarouba versicolor St. Hil. (fam. Simaroubaceae, determinar se há indução de resistência pela ingestão de pequenas e repetidas doses e, se a planta mantém sua toxicidade quando dessecada. Foram realizados dois experimentos, sendo o primeiro com folhas verdes ou folhas dessecadas e trituradas de S. versicolor em doses únicas de 5g/kg, 5g/kg e 3g/kg a três ovinos (Ovino 1, 2 e 3 respectivamente. O experimento 2, foi realizado com diferentes doses diárias de folhas dessecadas e trituradas de S. versicolor em quatro ovinos que receberam 1,5g/kg, 0,75g/kg, 0,6g/kg e 0,3g/kg e, com um ovino que recebeu 3g/kg como controle positivo (Ovino 4. A administração foi suspensa quando os ovinos apresentaram sinais clínicos da intoxicação. Após doze dias de recuperação, os animais sobreviventes foram desafiados com a mesma dose diária da planta ingerida anteriormente para avaliar o desenvolvimento de resistência. Os sinais clínicos observados nos dois experimentos caracterizaram-se por anorexia, mucosas oculares congestas, polidipsia, sialorreia, fezes pastosas que evoluíram para diarreia líquida fétida esverdeada, decúbito lateral e morte para os Ovinos 1 a 7. As principais lesões histológicas observadas foram necrose do tecido linfoide (linfonodos, baço, placas de Peyer e enterite necrosante. Com os resultados obtidos, pode-se concluir que os ovinos podem ser utilizados como modelo experimental clínico-patológico na intoxicação por S. versicolor. Com o método utilizado, não houve resistência ao consumo diário de folhas

  18. Production of laccase by Coriolus versicolor and its application in decolorization of dyestuffs: (Ⅱ) Decolorization of dyes by laccase containing fermentation broth with or without self-immobilized mycelia

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The capability of decolorization for commercial dyes byCoriolus versicolor fermentation broth containing laccase with orwithout immobilized mycelium was evaluated. With cell-freefermentation broth containing laccase, high decolorization ratiowas achieved for acid orange 7, but not for the other dyesconcerned. The immobilized mycelium was proved to be more efficientthan the cell-free system. All the four dyestuffs studied werefound being decolourized with certain extent by immobilizedmycelium. The repeated-batch decolorization was carried out withsatisfactory results. The experimental data showed that thecontinuous decolorization of wastewater from a printing and dyeingindustry was possible by using the self-immobilized C. Versicolor.

  19. Produção, isolamento e caracterização química do exopolissacarídeo do fungo da podridão branca da madeira Tramentes versicolor

    OpenAIRE

    Pereira, Susana Raquel de Sousa

    2009-01-01

    O Trametes versicolor é um fungo da podridão branca da madeira com potencial aplicação como oxidante na indústria papeleira. Segundo a literatura, este fungo produz também uma (1-3)--glucana com actividade imunomoduladora, propriedades anti-tumorais e anti-microbianas. O objectivo deste trabalho foi produzir, isolar, solubilizar e caracterizar quimicamente o exopolissacarídeo (EPS) do fungo T. versicolor. O fungo foi produzido por fermentação em cultura líquida, tendo o material polimérico s...

  20. Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

    Science.gov (United States)

    Libert, X; Chasseur, C; Bladt, S; Packeu, A; Bureau, F; Roosens, N H; De Keersmaecker, S C J

    2015-09-01

    Currently, contamination of indoor environment by fungi and molds is considered as a public health problem. The monitoring of indoor airborne fungal contamination is a common tool to help understanding the link between fungi in houses and respiratory problems. Classical analytical monitoring methods, based on cultivation and microscopic identification, depend on the growth of the fungi. Consequently, they are biased by difficulties to grow some species on certain culture media and under certain conditions or by noncultivable or dead fungi that can consequently not be identified. However, they could have an impact on human health as they might be allergenic. Since molecular methods do not require a culture step, they seem an excellent alternative for the monitoring of indoor fungal contaminations. As a case study, we developed a SYBR® green real-time PCR-based assay for the specific detection and identification of Aspergillus versicolor, which is frequently observed in indoor environment and known to be allergenic. The developed primers amplify a short region of the internal transcribed spacer 1 from the 18S ribosomal DNA complex. Subsequently, the performance of this quantitative polymerase chain reaction (qPCR) method was assessed using specific criteria, including an evaluation of the selectivity, PCR efficiency, dynamic range, and repeatability. The limit of detection was determined to be 1 or 2 copies of genomic DNA of A. versicolor. In order to demonstrate that this SYBR® green qPCR assay is a valuable alternative for monitoring indoor fungal contamination with A. versicolor, environmental samples collected in contaminated houses were analyzed and the results were compared to the ones obtained with the traditional methods.

  1. Possible origin of B chromosome in Dichotomius sericeus (Coleoptera).

    Science.gov (United States)

    Amorim, Igor Costa; Milani, Diogo; Cabral-de-Mello, Diogo Cavalcanti; Rocha, Marília França; Moura, Rita Cássia

    2016-08-01

    B chromosomes have so far been described in about 80 species of Coleoptera, mainly using conventional staining analysis. In this study, 152 individuals of the dung beetle Dichotomius sericeus (Coleoptera), collected from three isolated geographical areas in the State of Pernambuco, Brazil, were analyzed to determine the frequency, prevalence, distribution, meiotic behavior, and possible B chromosome origin. The cytogenetic analysis consisted of conventional staining, C-banding, triple fluorochrome staining (CMA3/DA/DAPI), and fluorescent in situ hybridization using ribosomal DNAs (rDNAs) and H3 histone gene as probes, as well as microdissection and chromosome painting of the B chromosome. The B chromosomes were detected in all populations analyzed. Analysis revealed the heterochromatic nature and the presence of G+C-rich blocks and 18S rDNA on the B chromosome. FISH with DNA from microdissected B chromosome painted the entire extension of the B chromosome for all populations, besides the pericentromeric regions of all the autosomes, as well as the X chromosome. Finally, cross-hybridization in nine related species of Dichotomius using the microdissected B chromosome as probe did not reveal any hybridization signal. The results suggest an intraspecific and monophyletic origin for B chromosomes in D. sericeus, probably from the second or third autosomal pair.

  2. The use of Trametes versicolor laccase for the polymerization of aniline in the presence of vesicles as templates.

    Science.gov (United States)

    Junker, Katja; Kissner, Reinhard; Rakvin, Boris; Guo, Zengwei; Willeke, Martin; Busato, Stephan; Weber, Thomas; Walde, Peter

    2014-02-01

    The enzymatic polymerization of aniline to polyaniline (PANI) with Trametes versicolor laccase (TvL) as catalyst and dioxygen (O₂) as oxidant was investigated in an aqueous medium containing unilamellar vesicles with an average diameter of about 80 nm formed from AOT (=sodium bis(2-ethylhexyl) sulfosuccinate). Compared to the same reaction carried out with horseradish peroxidase isoenzyme C (HRPC) as catalyst and hydrogen peroxide (H₂O₂) as oxidant, notable differences were found in the kinetics of the reaction, as well as in the characteristics of the PANI obtained. Under comparable optimal conditions, which are pH 3.5 for TvL/O₂ and pH 4.3 for HRPC/H₂O₂, the reaction with TvL/O₂ was much slower than with HRPC/H₂O₂, i.e. ≈27 days vs. 1 day reaction time to reach equilibrium with >90% yield at 25 °C. Although in both cases, aniline monomer coupling occurred mainly via the carbon atom in para position of aniline, UV-vis-NIR absorption and EPR measurements indicate that the reaction with TvL/O₂ yielded mainly overoxidized products (with λ(max)=730 nm). These products had a lower amount of unpaired electrons if compared with the products obtained with HRPC/H₂O₂ (with λ(max)≈1000 nm, which is characteristic for the polaron state of PANI-ES, the emeraldine salt form of PANI). Similarly to previous findings with HRPC/H₂O₂, enzyme inactivation occurred during the polymerization also in the case of TvL/O₂. Since the aqueous PANI-vesicle suspensions obtained are of high colloidal stability, they can be used directly as ink in a conventional thermal inkjet printer for printing on paper or on surface treated polyimide films. Printed PANI-ES patterns on paper changed colour from green (emeraldine salt) to blue (emeraldine base) upon exposure to ammonia gas, demonstrating the expected ammonia sensing properties.

  3. Differential patterns of accumulation and depuration of dietary selenium and vanadium during metamorphosis in the Gray Treefrog (Hyla versicolor).

    Science.gov (United States)

    Rowe, Christopher L; Heyes, Andrew; Hilton, Jessica

    2011-02-01

    Selenium (Se) and vanadium (V) are contaminants commonly found in aquatic systems affected by wastes derived from fossil fuels. To examine their effects on a widely distributed species of amphibian, we exposed gray tree frogs (Hyla versicolor) to Se (as SeO₂) or V (as NaVO₃) in their diet from the early larval period to metamorphosis. Concentrations of Se in Se-enriched food were 1.0 (Se control), 7.5 (Se low), and 32.7 (Se high) μg/g dw. Concentrations of V in V-enriched food were 3.0 (V control), 132.1 (V low), and 485.7 (V high) μg/g dw. Although we observed bioaccumulation of both metals throughout the larval period, no effects on growth, survival, metabolic rate, or lipid content were observed. Se concentrations in tissues did not vary among life stages, neither in Se low nor Se high treatments, such that maximum accumulation had occurred by the mid-larval period. In addition, there was no evidence of depuration of Se in either the Se low or the Se high treatments during metamorphosis. A strikingly different pattern of accumulation and depuration occurred in V-exposed individuals. In treatments V low and V high, maximum body burdens occurred in "premetamorphs" (i.e., animals with developed forelimbs but in which tail resorption had not begun), whereas body burdens in animals having completed metamorphosis were much lower and similar to those in larvae. These results suggest that compared with Se-exposed animals, V-exposed animals were able to depurate a substantial amount of accumulated V during the metamorphic period. In an ecologic context, it appears that amphibians exposed to Se during the larval period may serve as a vector of the metal to terrestrial predators, yet potential transfer of accumulated V to predators would largely be restricted to the aquatic habitat.

  4. Kinetics of wheat straw solid-state fermentation with Trametes versicolor and Pleurotus ostreatus - lignin and polysaccharide alteration and production of related enzymatic activities

    Energy Technology Data Exchange (ETDEWEB)

    Valmaseda, M.; Martinez, M.J.; Martinez, A.T. (Consejo Superior de Investigaciones Cientificas, Madrid (Spain). Centro des Investigaciones Biologicas)

    1991-09-01

    The kinetics of straw solid-state fermentation (SSF) with Trametes versicolor and Pleurotus ostreatus was investigated to characterize the delignification processes by these white-rot fungi. Two sucessive phases could be defined during straw transformation, characterized by changes in respiratory activity, changes in lignin and polysaccharide content and composition, increase in in-vitro digestibility, and enzymatic activities produced by the fungi. Lignin composition was analysed after CuO alkaline degradation, and decreases in syringyl/guaiacyl and syringyl/p-hydroxyphenyl ratios and cinnamic acid content were observed during the fungal treatment. An increase in the phenolic acid yield, revealing fungal degradation of sidechains in lignin, was produced by P. ostreatus. The highest xylanase level was produced by P. ostreatus, and exocellulase activity was nearly absent from straw treated with this fungus. Laccase activity was found in straw treated with both fungi, but lignin peroxidase was only detected during the initial phase of straw transformation with T. versicolor. High levels of H{sub 2}O{sub 2}-producing acryl-alcohol oxidase occurred throughout the straw SSF with P. ostreatus. (orig.).

  5. Characterization of purified and Xerogel immobilized Novel Lignin Peroxidase produced from Trametes versicolor IBL-04 using solid state medium of Corncobs

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    Asgher Muhammad

    2012-08-01

    Full Text Available Abstract Background Cost-effective production of industrially important enzymes is a key for their successful exploitation on industrial scale. Keeping in view the extensive industrial applications of lignin peroxidase (LiP, this study was performed to purify and characterize the LiP from an indigenous strain of Trametes versicolor IBL-04. Xerogel matrix enzyme immobilization technique was applied to improve the kinetic and thermo-stability characteristics of LiP to fulfil the requirements of the modern enzyme consumer sector of biotechnology. Results A novel LiP was isolated from an indigenous T. versicolor IBL-04 strain. T. versicolor IBL-04 was cultured in solid state fermentation (SSF medium of corn cobs and maximum LiP activity of 592 ± 6 U/mL was recorded after five days of incubation under optimum culture conditions. The crude LiP was 3.3-fold purified with specific activity of 553 U/mg after passing through the DEAE-cellulose and Sephadex-G-100 chromatography columns. The purified LiP exhibited a relatively low molecular weight (30 kDa homogenous single band on native and SDS-PAGE. The LiP was immobilized by entrapping in xerogel matrix of trimethoxysilane (TMOS and proplytetramethoxysilane (PTMS and maximum immobilization efficiency of 88.6% was achieved. The free and immobilized LiPs were characterized and the results showed that the free and immobilized LiPs had optimum pH 6 and 5 while optimum temperatures were 60°C and 80°C, respectively. Immobilization was found to enhance the activity and thermo-stability potential of LiP significantly and immobilized LiP remained stable over broad pH and temperature range as compare to free enzyme. Kinetic constants Km and Vmax were 70 and 56 μM and 588 and 417 U/mg for the free and immobilized LiPs, respectively. Activity of this novel extra thermo-stable LiP was stimulated to variable extents by Cu2+, Mn2+ and Fe2+ whereas, Cystein, EDTA and Ag+ showed inhibitory effects

  6. Vertical stratification of beetles (Coleoptera) and flies (Diptera) in temperate forest canopies.

    Science.gov (United States)

    Maguire, Dorothy Y; Robert, Katleen; Brochu, Kristen; Larrivée, Maxim; Buddle, Christopher M; Wheeler, Terry A

    2014-02-01

    Forest canopies support high arthropod biodiversity, but in temperate canopies, little is known about the spatial distribution of these arthropods. This is an important first step toward understanding ecological roles of insects in temperate canopies. The objective of this study was to assess differences in the species composition of two dominant and diverse taxa (Diptera and Coleoptera) along a vertical gradient in temperate deciduous forest canopies. Five sugar maple trees from each of three deciduous forest sites in southern Quebec were sampled using a combination of window and trunk traps placed in three vertical strata (understory, mid-canopy, and upper-canopy) for three sampling periods throughout the summer. Coleoptera species richness and abundance did not differ between canopy heights, but more specimens and species of Diptera were collected in the upper-canopy. Community composition of Coleoptera and Diptera varied significantly by trap height. Window traps collected more specimens and species of Coleoptera than trunk traps, although both trap types should be used to maximize representation of the entire Coleoptera community. There were no differences in abundance, diversity, or composition of Diptera collected between trap types. Our data confirm the relevance of sampling all strata in a forest when studying canopy arthropod biodiversity. PMID:24472199

  7. Vertical stratification of beetles (Coleoptera) and flies (Diptera) in temperate forest canopies.

    Science.gov (United States)

    Maguire, Dorothy Y; Robert, Katleen; Brochu, Kristen; Larrivée, Maxim; Buddle, Christopher M; Wheeler, Terry A

    2014-02-01

    Forest canopies support high arthropod biodiversity, but in temperate canopies, little is known about the spatial distribution of these arthropods. This is an important first step toward understanding ecological roles of insects in temperate canopies. The objective of this study was to assess differences in the species composition of two dominant and diverse taxa (Diptera and Coleoptera) along a vertical gradient in temperate deciduous forest canopies. Five sugar maple trees from each of three deciduous forest sites in southern Quebec were sampled using a combination of window and trunk traps placed in three vertical strata (understory, mid-canopy, and upper-canopy) for three sampling periods throughout the summer. Coleoptera species richness and abundance did not differ between canopy heights, but more specimens and species of Diptera were collected in the upper-canopy. Community composition of Coleoptera and Diptera varied significantly by trap height. Window traps collected more specimens and species of Coleoptera than trunk traps, although both trap types should be used to maximize representation of the entire Coleoptera community. There were no differences in abundance, diversity, or composition of Diptera collected between trap types. Our data confirm the relevance of sampling all strata in a forest when studying canopy arthropod biodiversity.

  8. BESOUROS COPRÓFAGOS (COLEOPTERA; SCARABAEIDAE COLETADOS EM PIRACICABA, SP

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    RODRIGUES S.R.

    1998-01-01

    Full Text Available Através do uso de armadilhas "pitfall" iscadas com massa fecal fresca de bovinos, realizou-se a coleta de besouros coprófagos (Coleoptera; Scarabaeidae, durante o período de 15 de abril de 1995 a 17 de fevereiro de 1996, em área de pastagem ao lado de confinamento de bovinos, em Piracicaba, SP. Coletou-se um total de 11 espécies distribuidas nos gêneros Aphodius, Ataenius, Trichillum, Eurysternus, Dichotomius e Canthon. Os besouros coprófagos de comportamento endocoprídeo representaram 72,73% das espécies coletadas, sendo estes os de maior ocorrência e a espécie Aphodius lividus parece ser a mais bem adaptada na área estudada.

  9. Brachylophora, a new brachypterous genus of Rhopalophorini (Coleoptera, Cerambycidae

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    Robin O. S. Clarke

    2011-06-01

    Full Text Available Brachylophora, a new brachypterous genus of Rhopalophorini (Coleoptera, Cerambycidae. Brachylophora auricollis (Bruch, 1918 comb. nov. = Pasiphyle auricollis Bruch, 1918, originally described from Argentina (Salta, is redescribed and illustrated. Although with reduced elytra, the genus is transferred from Rhinotragini to Rhopalophorini based on the following characters: eyes well separated in both sexes, frons between eyes depressed and lacking frontal suture; pro-, meso-, and metasternum planar; mesothorax parallel-sided, not at all declivous before mesosternal process; metasternum large, together with mesosternum twice length of prosternum, metepisternum very wide, entire suture separating it from metasternum clearly visible when viewed from below; female ovipositor shortened with short cylindrical styles; and, more generally, structural features of hind legs, and surface ornamentation. Habitus similar to Coremia group. Bolivian specimens were netted as they visited flowers of Croton sp. (Euphorbiaceae.

  10. Coleoptera associated with macrophytes of the genus Salvinia in four oxbow lakes in two river basins in southeast Brazil.

    Science.gov (United States)

    Paula-Bueno, M C; Fonseca-Gessner, A A

    2015-11-01

    Macrophytes in oxbow lakes represent an important substrate for the Coleoptera. Two oxbow lakes the Rio Paranapanema were studied and the other two Rio Mogi-Guaçu, in the State de São Paulo, Brasil. In this study, there is greater similarity between the communities of Coleoptera of lakes greater connectivity with the main river channel or the difference in the species of Salvinia collected in the lakes studied interferes Coleoptera fauna that uses as substrate. A total of 9,222 specimens of Coleoptera were collected and identified in 10 families and 40 genera. The analysis MDS for abundance of Coleoptera showed the grouping of the oxbow lakes the Paranapanema River and a distancing the oxbow lakes the Mogi-Guaçu. The PERMANOVA test did not reveal any difference in the fauna between the wet and dry periods. It was concluded that the connectivity between river and lake is not decisive for the richness and abundance of aquatic fauna of Coleoptera. Therefore, the richness and abundance of aquatic Coleoptera associated vary with the species of Salvinia used as substrate.

  11. Coleoptera associated with macrophytes of the genus Salvinia in four oxbow lakes in two river basins in southeast Brazil.

    Science.gov (United States)

    Paula-Bueno, M C; Fonseca-Gessner, A A

    2015-11-01

    Macrophytes in oxbow lakes represent an important substrate for the Coleoptera. Two oxbow lakes the Rio Paranapanema were studied and the other two Rio Mogi-Guaçu, in the State de São Paulo, Brasil. In this study, there is greater similarity between the communities of Coleoptera of lakes greater connectivity with the main river channel or the difference in the species of Salvinia collected in the lakes studied interferes Coleoptera fauna that uses as substrate. A total of 9,222 specimens of Coleoptera were collected and identified in 10 families and 40 genera. The analysis MDS for abundance of Coleoptera showed the grouping of the oxbow lakes the Paranapanema River and a distancing the oxbow lakes the Mogi-Guaçu. The PERMANOVA test did not reveal any difference in the fauna between the wet and dry periods. It was concluded that the connectivity between river and lake is not decisive for the richness and abundance of aquatic fauna of Coleoptera. Therefore, the richness and abundance of aquatic Coleoptera associated vary with the species of Salvinia used as substrate. PMID:26602356

  12. Diversidad de la familia Carabidae (Coleoptera en Chile Diversity of the family Carabidae (Coleoptera in Chile

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    SERGIO ROIG-JUÑENT

    2001-09-01

    Full Text Available Carabidae constituye dentro de los coléopteros chilenos, la cuarta familia en importancia por su cantidad de especies. El presente trabajo incluye una breve compilación acerca de la historia de la familia y de las primeras expediciones realizadas en Chile. También se realizan comparaciones de la diversidad de carábidos chilenos con respecto a otros países y el Neotrópico. Para Chile, se conocen 21 tribus, con 95 géneros y 365 especies, que representan el 38,8, 28,8 y 7,9 % de la fauna del Neotrópico, respectivamente. Chile posee un bajo número de tribus comparado con otros países, sin embargo, constituye un área importante por la presencia de seis tribus relictuales, principalmente pangeicas o gondwánicas. Chile posee 18 géneros endémicos (18,5 % de su fauna de Carabidae, 28 cuya distribución está restringida a Chile y Argentina y seis restringidos a Chile, Argentina y Uruguay. La cantidad de especies presentes en Chile es inferior a la que poseen otros países de América del Sur, pero la cantidad de especies endémicas es muy alta (204 y representa el 55,8 % de su fauna de carábidos. El alto grado de endemismo que posee Chile con respecto a otros países de América del Sur puede deberse a su condición de aislamiento, siendo las barreras más importantes la región desértica del norte y la cordillera de Los Andes. Este hecho también se vislumbra por la ausencia de importantes tribus neotropicales como Galeritini, Scaritini y Brachinini. También se incluyen en este trabajo claves para la identificación de todas las tribus y géneros presentes en Chile, como así también una breve descripción acerca de la diversidad y ambientes en los que se encuentra cada géneroThe family Carabidae is the fourth largest Coleoptera family in Chile. The present work includes a brief compilation on the taxonomic history of the family and the first expeditions to Chile. In addition, knowledge of carabid diversity in Chile is compared with

  13. Walking stability of Rhyzopertha dominica (Fabricius, 1792) (Coleoptera: Bostrichidae).

    Science.gov (United States)

    Pires, E M; Nogueira, R M; Pina, D S; Manica, C L M; Faroni, L R A; Moreira, P S A

    2016-04-19

    Results obtained in studies can contribute to the advancement of science and innovative methods and techniques for developing practical activities. Reporting conditions that may restrict the implementation of research is critical to ensure the optimal development of further technical studies. The objective of this study was to assess the walking stability of R. dominica on a flat and smooth surface. The study was based on the determination of mortality, morphology and walking stability of the insect outside the grain mass, on a flat and smooth surface. Mortality of adults of this Coleoptera in conditions with and without food was similar, which explains the difficulty that this insect had for accessing the food source on the flat and smooth surface. The measurements of body length (BOL), width (BOW) and height (BOH) of R. dominica were compared with those of Tribolium castaneum (Coleoptera: Tenebrionidae), which showed good ability to walk in these conditions. This study indicated that the former presents lower BOL and BOW, and greater BOH than the second, and all these variables showed differences when analyzed simultaneously by means of the construction of multivariate morphometric indices (Width × Height, Length × Height and Height × Length × Width). These morphometric variables, together with the definition of the geometry most similar to the body shape, resulted in determination of the center of gravity (CG) and static rollover threshold (SRTgeom) for both species. Rhyzopertha dominica and T. castaneum presented CGs considered high and low, respectively, and together with the values obtained for SRTgeom, may justify that R. dominica can be considered a less stable species during movement, and presents greater risk of rollover on flat and smooth surfaces. PMID:27097085

  14. Track analysis of the Neotropical Entimini (Coleoptera: Curculionidae: Entiminae

    Directory of Open Access Journals (Sweden)

    Alberto Romo

    2011-09-01

    Full Text Available Track analysis of the Neotropical Entimini (Coleoptera, Curculionidae, Entiminae. Distributional patterns of the species belonging to the tribe Entimini from the Neotropical region were analyzed. Based on a track analysis of 22 species of Entimus, Rhigus, and Phaedropus, for which distributional data were available, two generalized tracks were found. One is located in northern Brazil, corresponding to the Amazonian subregion, and is determined by Phaedropus candidus and Rhigus speciosus. The other is located in southern Brazil, corresponding to the Parana subregion, and is determined by Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus, and R. tribuloides. The development of the Chacoan subregion is hypothesized to have been the dynamic vicariant event that fragmented the former Amazonian-Parana forest.Análisis de trazos de Entimini Neotropical (Coleoptera, Curculionidae, Entiminae. Se analizaron los patrones de distribución de la tribu Entimini en la región Neotropical. Con base en un análisis de los trazos de 22 especies de Entimus, Rhigus y Phaedropus, para los cuales se contaba con datos de distribución, se hallaron dos trazos generalizados. Uno se localiza en el norte de Brasil, en la subregión Amazónica, y está sustentado por Phaedropus candidus y Rhigus speciosus. El otro se localiza en el sur de Brasil, en la subregión Paranaense, y está sustentado por Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus y R. tribuloides. Se hipotetiza que el desarrollo de la subregión Chaqueña constituyó el evento de vicarianza dinámica que fragmentó el antiguo bosque Amazónico-Paranaense.

  15. Diversity of forensic rove beetles (Coleoptera, Staphylinidae) associated with decaying pig carcass in a forest biotope.

    Science.gov (United States)

    Dekeirsschieter, Jessica; Frederick, Christine; Verheggen, Francois J; Drugmand, Didier; Haubruge, Eric

    2013-07-01

    Most forensic studies are focused on Diptera pattern colonization while neglecting Coleoptera succession. So far, little information is available on the postmortem colonization by beetles and the decomposition process they initiate under temperate biogeoclimatic countries. These beetles have, however, been referred to as being part of the entomofaunal colonization of a dead body. Forensic entomologists need increased databases detailing the distribution, ecology, and phenology of necrophagous insects, including staphylinids (Coleoptera, Staphylinidae). While pig carcasses are commonly used in forensic entomology studies to surrogate human decomposition and to investigate the entomofaunal succession, very few works have been conducted in Europe on large carcasses. Our work reports the monitoring of the presence of adult rove beetles (Coleoptera, Staphylinidae) on decaying pig carcasses in a forest biotope during four seasons (spring, summer, fall, and winter). A total of 23 genera comprising 60 species of rove beetles were collected from pig carcasses.

  16. 载体吸附培养黄孢原毛平革菌(Phanerochaete chrysosporium)和云芝(Trametes versicolor) 对染料的脱色%Decolorization of dyes by Phanerochaete chrysosporium and Trametes versicolor growing on adsorptive carriers

    Institute of Scientific and Technical Information of China (English)

    张昊; 李学梅; 张文黎; 花清信; 苌道松; 徐颖; 李雪玲; 任四伟; 陈宣宇; 杨清香

    2012-01-01

    Phanerochaete chrysosporium and Trametes versicolor were cultured on different carriers for continuous decolorization, the performance of different Phanerochaete chrysosporium and Trametes versicolor were researched and compared. In this paper, Phanerochaete chrysosporium and Trametes versicolor were cultured in liquid containing spherical sawdust, corncob and peanut shells under oscillation conditions. During few days of cultivation, large amount of bacteria was grew and attached on the carrier surface in membranous and lump state. After consecutive 4 rounds of decolorization, Phanerochaete chrysosporium and Trametes versicolor growing on sawdust carrier had significant advantage both in continuous decolorization and producing enzymes, so the optimal adsorptive carrier was sawdust. After consecutive 2 rounds and 12 d of reactive black RB5 decolorization, the Phanerochaete chrysosporium culture with sawdust as adsorptive carrier could achieve the decolorization of 97 %; after three rounds of dye supplemented, the cultures also could remove nearly 96% of M-3BE and the maximum production of manganese dependant pcroxidase enzyme (MnP) and lignin peroxidase enzyme (LiP) was 611, 1 477 U/L. In the actual application, Phanerochaete chrysosporium and Trametes versicolor culture with sawdust as carriers should be added to dye wastewater treatment system to strengthen the biological treatment effect.%比较研究了不同载体吸附培养的黄孢原毛平革菌(Phanerochaete chrysosporium)和云芝(Trametes versicolor)对染料连续脱色的效果.结果表明:(1)白腐真菌黄孢原毛平革菌和云芝分别在含有球形的木屑、玉米芯和花生壳载体的液体环境中振荡培养,菌体以膜状或团状形式大量附着生长在载体表面.(2)连续4轮脱色过程中,不论黄孢原毛平革菌还是云芝,都是木屑为载体的培养液的持续脱色和产酶能力最好,宜选择木屑为载体.其中,木屑为载体的黄孢

  17. Pityriasis versicolor: clinical-epidemiological characterization of patients in the urban area of Buerarema-BA , Brazil Pitiríase versicolor: caracterização clínico-epidemiológica em pacientes da área urbana de Buerarema-BA, Brasil

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    Juliano Oliveira Santana

    2013-04-01

    Full Text Available BACKGROUND: Pityriasis versicolor (PV is a cutaneous pigmentation disorder caused by a lipophilic yeast of the genus Malassezia sp. It is a superficial mycosis characterized by well-defined, slightly scaly skin lesions of variable color. In Brazil, the number of reported cases is small, and there are few epidemiological studies. OBJECTIVES: to assess incidence, characteristics of the lesions, effectiveness of the Zileri's Sign procedure, and the epidemiological profile of PV in the urban area of Buerarema - Bahia. METHODS: Biological samples were collected on pre-established days at Basic Health Care Units from July to September 2010. Sample collection was followed by laboratory diagnosis using Porto's Method. RESULTS: Of the 158 patients with suspected PV participating in the study, 105 (66.5% were positive; 72 (68.6% were female and 33 (31.4% were male. Sex and location of lesions showed statistically significant differences (p FUNDAMENTOS: A pitiríase versicolor é um distúrbio de pigmentação cutânea causada pela levedura lipofílica do gênero Malassezia sp. É uma micose superficial caracterizada por produzir lesões delimitadas, com descamação fina e de cor variável. No Brasil, o relato do número de casos e de estudos é restrito. OBJETIVOS: Analisar a incidência, as características das lesões, a eficácia do Sinal de Zileri e o perfil epidemiológico da pitiríase versicolor na área urbana do município de Buerarema-BA. MÉTODOS: Foram coletadas amostras biológicas no período de julho a setembro de 2010, em dias pré-establecidos, nas Unidades Básicas de Saúde. O Método de Porto foi utilizado como diagnóstico laboratorial. RESULTADOS: Dos 158 pacientes cadastrados com suspeita de pitiríase versicolor, 105 (66,5% mostraram-se positivos, sendo 72 (68,6% do sexo feminino e 33 (31,4% do sexo oposto. Ocorreu diferença estatisticamente significante entre sexo e localização das lesões (p<0,05. A região com maior

  18. Influence of growing location and cultivar on Rhyzopertha dominica (Coleoptera: Bostrichidae) and Sitophilus oryzae (Coleoptera: Curculionidae) infestation of rough rice

    Institute of Scientific and Technical Information of China (English)

    FRANK H. ARTHUR; RUSTY C. BAUTISTA; TERRENCE J. SIEBENMORGEN

    2007-01-01

    Long-grain rice cultivars Cocodrie, Wells, and XP 723 grown in three locations (Hazen, MO; Essex and Newport, AR, USA), and medium-grain rice cultivars Bengal and XP 713 grown in two locations (Jonesboro and Lodge Corner, AR, USA), were harvested and assayed for susceptibility to Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), the lesser grain borer, and Sitophilus oryzae (L.) (Coleoptera: Curculionidae), the rice weevil, on rice held at 27℃ 57% and 75% relative humidity (RH).Separate samples from the same harvest lots were also analyzed for the physical characteristics of brown rice yield, percentage whole kernels and kernel thickness. Progeny production and feeding damage of R. dominica were significantly different among long-grain cultivars within two of the three locations (P < 0.05), but not for location or RH (P ≥ 0.05), while progeny production of S. oryzae was different among cultivars, location, and RH (P < 0.05). On medium-grain rice, both cultivar and location were significant for progeny production of R. dominica, but not RH, while cultivar and RH were significant for progeny production of S. oryzae, but not location. On both rice types, feeding damage of R. dominica followed the same trends and was always strongly positively correlated with progeny production (P < 0.05), but for S. oryzae there were several instances in which progeny production was not correlated with feeding damage (P ≥ 0.05). Physical characteristics of both rice types were statistically significant (P < 0.01) but actual numerical differences were extremely small, and were generally not correlated with progeny production of either species. Results indicate that the location in which a particular rice cultivar is grown, along with its characteristics, could affect susceptibility of the rice to R. dominica and S. oryzae.

  19. Coleoptera species inhabiting prairie wetlands of the Cottonwood Lake Area, Stutsman County, North Dakota

    Science.gov (United States)

    Hanson, B.A.; Swanson, G.A.

    1989-01-01

    The aquatic Coleoptera of a prairie wetland complex in Stutsman County, North Dakota, were collected from April 1979 to November 1980. Identification of 2594 individuals confirmed 57 species, including seven new records for North Dakota. Two seasonally flooded and two semipermanent wetlands, totaling 7.43 ha, contained 53% of the Dytiscidae, 43% of the Haliplidae, 38% of the Hydrophilidae, and 22% of the Gyrinidae species previously identified from North Dakota. Although 49.1% of the Coleoptera species occurred in both types of wetlands, the occurrence of 29 species varied by wetland class.

  20. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae)

    OpenAIRE

    Dilma Solange Napp; Ubirajara Ribeiro Martins

    2006-01-01

    Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae). Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae). The following new genus and species, all from Bolivia, are describ...

  1. Optimization of C/N Ratio and Inducers for Wastewater Paper Industry Treatment Using Trametes versicolor Immobilized in Bubble Column Reactor

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    Aura M. Pedroza-Rodríguez

    2013-01-01

    Full Text Available C/N ratio and MnSO4 and CuSO4 concentrations were optimized for decolorization and chemical oxygen demand (COD removal of bleached Kraft pulp mill effluent by Trametes versicolor immobilized in polyurethane foam. Statistical differences (P<0.0001 at high C/N ratios (169, 2 mM CuSO4, and 0.071 mM MnSO4 were determined. Decolorization of 60.5%, COD removal of 55%, laccase (LAC 60 U/L, and manganese peroxidase (MnP 8.4 U/L were obtained. Maximum of decolorization (82%, COD removal (83%, LAC (443.5 U/L, and MnP (18 U/L activities at C/N ratio of 405 (6.75 mM CuSO4 and 0.22 mM MnSO4 was achieved in step 7 at 4 d. Positive correlation between the decolorization, COD removal, and enzymatic activity was found (P<0.0001. T. versicolor bioremediation capacity was evaluated in bubble column reactor during 8 d. Effluent was adjusted according to optimized parameters and treated at 25°C and air flow of 800 mL/min. Heterotrophic bacteria growth was not inhibited by fungus. After 4 d, 82% of COD reduction and 80% decolorization were recorded. Additionally, enzymatic activity of LAC (345 U/L and MnP (78 U/L was observed. The COD reduction and decolorization correlated positively (P<0.0001 with enzymatic activity. Chlorophenol removal was 98% of pentachlorophenol (PCP, 92% of 2,4,5-trichlorophenol (2,4,5-TCP, 90% of 3,4-dichlorophenol (3,4-DCP, and 99% of 4-chlorophenols (4CP.

  2. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis

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    Sri Puji Astuti Wahyuningsih

    2016-03-01

    Full Text Available Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1 and adding polysaccharide krestin before-after exposure (P3 had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2 had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1, 12-17.

  3. Comparison of Coleoptera emergent from various decay classes of downed coarse woody debris in Great Smoky Mountains National Park, USA

    OpenAIRE

    Ferro, Michael L.; Gimmel, Matthew L.; Harms, Kyle E.; Carlton, Christopher E.

    2013-01-01

    Coleoptera species composition and succession in downed woody debris habitats are poorly known in eastern North America. A photoeclector emergence chamber was used to concentrate Coleoptera that emerged from various decay classes of fine and coarse woody debris (FWD and CWD, respectively) collected in primary and secondary forest sites in Great Smoky Mountains National Park, Tennessee, USA. A total of 5673 adult beetle specimens, representing 305 lowest identifiable taxa within 227 genera and...

  4. Treatment of pityriasis versicolor with topical application of essential oil of Cymbopogon citratus (DC Stapf - therapeutic pilot study Tratamento de pitiríase versicolor com aplicação tópica do óleo essencial de Cymbopogon citratus (DC Stapf - estudo terapêutico piloto

    Directory of Open Access Journals (Sweden)

    Egberto Santos Carmo

    2013-06-01

    Full Text Available BACKGROUND: Pityriasis versicolor is a fungal infection caused by Malassezia spp. that has frequent relapses. OBJECTIVES: The main objective of this research was to perform phase I and II clinical studies, using formulations containing essential oil of Cymbopogon citratus in patients with pityriasis versicolor. METHODS: Phase I study included twenty volunteers to ascertain the safety of the formulations. In phase II, 47 volunteers randomly received essential oil formulations at 1.25 μL/mL concentration, for forty days. The shampoo should be applied three times a week and the cream twice a day. A control group in phase II, consisting of 29 volunteers, received the same formulations but with 2% ketoconazole as the active ingredient. RESULTS: No significant adverse events were observed in volunteers during Phase I. In Phase II, 30 (63.83% volunteers using essential oil and 18 (62.07% using ketoconazole remained until the end of the study. We observed a predominance of lesions in disseminated form, with M. sympodialis detected as the predominant agent identified in cultures. After 40 days of treatment, the rate of mycological cure was 60% (p FUNDAMENTOS: Pitiríase versicolor é uma micose causada pela Malassezia spp., e que apresenta frequentes recidivas. OBJETIVOS: Este trabalho objetivou realizar estudos clínicos de fase I e II, para essa patologia, com óleo essencial de Cymbopogon citratus. MÉTODOS: Na fase I, participaram vinte voluntários para averiguar a segurança das formulações. Na fase II, 47 voluntários receberam as formulações do óleo essencial a 1,25 μL/mL, as quais deveriam ser utilizadas por quarenta dias, sendo o xampu três vezes por semana e o creme duas vezes ao dia. Um grupo controle na fase II, constituído por 29 voluntários recebeu as mesmas formulações, com cetoconazol a 2%. RESULTADOS: Verificada a segurança das formulações com a finalização da fase I, onde nenhuma reação adversa significativa foi

  5. Contribution to the knowledge of seed-beetles (Coleoptera, Chrysomelidae, Bruchinae) in Xinjiang, China

    Science.gov (United States)

    Li, You; Wang, Zhiliang; Guo, Jianjun; Nápoles, Jesús Romero; Ji, Yingchao; Jiang, Chunyan; Zhang, Runzhi

    2014-01-01

    Abstract Nineteen species of seed-beetles belonging to the subfamily Bruchinae (Coleoptera, Chrysomelidae) were collected in Xinjiang, China. Of these, the following four were new records for China: Bruchus affinis Frolich, 1799, Bruchus atomarius L., 1761, Bruchus loti Paykull, 1800 and Kytorhinus kergoati Delobel & Legalov, 2009. We provide an annotated checklist, illustrations and a key to the 19 species. PMID:25610333

  6. Upper lethal temperature limits of the common furniture beetle Anobium punctatum (Coleoptera: Anobiidae)

    DEFF Research Database (Denmark)

    Hansen, Lise Stengård; Jensen, Karl-Martin Vagn

    1996-01-01

    The susceptibility of the egg, larval and adult stages of Anobium punctatum De Geer (Coleoptera: Anobiidae) to heat (46-54°C, 25-30% RH) was investigated. The larval stage was found to be most tolerant to heat. Very short exposure (5 min) of the larvae to temperatures of 52°C and above led to 100...

  7. New record of predatory ladybird beetle (Coleoptera, Coccinellidae feeding on extrafloral nectaries

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    Lúcia M. Almeida

    2011-09-01

    Full Text Available New record of predatory ladybird beetle (Coleoptera, Coccinellidae feeding on extrafloral nectaries. Feeding by Exoplectra miniata (Germar on extrafloral nectaries of Inga edulis Mart. was observed in Nova Friburgo, Rio de Janeiro, Brazil. This is the first record of this behavior for Exoplectrini.

  8. The genus Platytenerus Miyatake, 1985 (Coleoptera: Cleridae: Neorthopleurinae), with description of a new species from Japan.

    Science.gov (United States)

    Murakami, Hiroyuki

    2015-03-23

    The genus Platytenerus Miyatake, 1985 (Coleoptera: Cleridae) is redescribed and classified into the subfamily Neorthopleurinae Opitz, 2009. A phylogenetic tree is supplementally provided for Platytenerus based on twenty morphological and two geographical characters. A new species of the genus, Platytenerus iriomotensis sp. n. is described from Iriomote Island, Okinawa, Japan.

  9. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae from Korea

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    Ik Je Choi

    2016-03-01

    Full Text Available A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  10. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae) from Korea

    OpenAIRE

    Ik Je Choi; Jongok Lim; Jinyoung Park; Ji Hwan Park; Jong Kyun Park

    2016-01-01

    A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae) is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  11. Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae), information on a little known taxon from Colombia

    OpenAIRE

    Kippenhan, Mike

    2011-01-01

    Two specimens of Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae) in the Field Museum of Natural History offer additional information on the morphology and distribution of this rare species. One of the specimens, a female, is considered to be the first known specimen of this species.

  12. Diapause and post-diapause quiescence demonstrated in overwintering Harmonia axyeidis (Coleoptera: Coccinellidae) in northwestern Europe

    NARCIS (Netherlands)

    Raak-van den Berg, C.L.; Jong, de P.W.; Hemerik, L.; Lenteren, van J.C.

    2013-01-01

    The Asian ladybird Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) is regarded as an invasive species in many parts of the world. In a previous study we hypothesised that H. axyridis enters diapause at the end of October and then shifts to a quiescent state in December in northwestern Europe.

  13. Chaves para a identificação dos principais Coleoptera (Insecta associados com produtos armazenados Keys for the identification of Coleoptera (Insecta associated with stored products

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Valle da Silva Pereira

    2001-03-01

    Full Text Available An illustrated key to identify nine families of Coleoptera commonly found in stored products is presented. Keys for the identification of Anobiidae [Lasioderma serricorne (Fabricius, 1792, Stegobium paniceum (Linnaeus, 1761], Bruchidae [Acanthoscelides obtectus (Say, 1831, Zabrotes subfasciatus (Boheman, 1833], Curculionidae [Sitophilus oryzae (Linnaeus, 1763, S. zeamais Motschulsky, 1885], Silvanidae [Ahasverus advena (Waltl, 1832, Cathartus quadricollis (Guérin, 1892, Oryzaephilus mercator (Fauvel, 1889, O. surinamensis (Linnaeus, 1758] and Tenebrionidae [Gnathocerus cornutus (Fabricius, 1798, Tribolium castaneum (Herbst, 1797, T. confusum du Val, 1868] are also provided. These keys cover the most frequent Coleoptera found in stored products, specially grains, and are to the adult stage only. Illustrations of external morphology and general characteristics are provided for each species reported.

  14. ESPECIES DE DRYOPHTHORINAE (COLEOPTERA: CURCULIONIDAE ASOCIADAS A PLÁTANO Y BANANO (Musaspp. EN COLOMBIA Dryophthorinae Species (Coleoptera: Curculionidae Associted to Platain and Banana crops (Musaspp. in Colombia

    Directory of Open Access Journals (Sweden)

    PAULA A SEPÚLVEDA-CANO

    Full Text Available Se presenta una sinopsis de los escarabajos de la subfamilia Dryophthorinae (Coleoptera: Curculionidae asociados a cultivos de plátano y banano en Colombia. Adicionalmente se ofrecen claves ilustradas para las especies del país. Se registran seis especies asociadas a dichos cultivos: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.This synopsis is about beetle&#’;s subfamily Dryophthorinae (Coleoptera: Curculionidae associated to plantain and banana crops. Additionally keys illustrated for the species of the country are offered. Six species associated to these cultures are registered: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.

  15. Molecular Markers Detect Cryptic Predation on Coffee Berry Borer (Coleoptera: Curculionidae) by Silvanid and Laemophloeid Flat Bark Beetles (Coleoptera: Silvanidae, Laemophloeidae) in Coffee Beans.

    Science.gov (United States)

    Sim, Sheina B; Yoneishi, Nicole M; Brill, Eva; Geib, Scott M; Follett, Peter A

    2016-02-01

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is a serious pest of coffee worldwide. It was first detected in Hawai'i in 2010. Two predatory beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and Leptophloeus sp. (Coleoptera: Laemophloeidae), have been observed in H. hampei-infested coffee. Under laboratory conditions, colony-reared C. quadricollis and Leptophloeus sp. prey upon all life stages of H. hampei. However, the H. hampei life cycle occurs almost exclusively within a coffee bean obscured from direct observation. Thus, it is unknown if C. quadricollis and Leptophloeus sp. consume H. hampei as prey in the wild. To demonstrate predation of H. hampei by C. quadricollis and Leptophloeus sp., a molecular assay was developed utilizing species-specific primers targeting short regions of the mitochondrial COI gene to determine species presence. Using these primers, wild C. quadricollis and Leptophloeus sp. were collected and screened for the presence of H. hampei DNA using PCR. Analysis of collections from five coffee farms revealed predation of C. quadricollis and Leptophloeus sp. on H. hampei. Further laboratory testing showed that H. hampei DNA could be detected in predators for as long as 48 h after feeding, indicating the farm-caught predators had preyed on H. hampei within 2 d of sampling. This study demonstrates the utility of molecular markers for the study of the ecology of predators and prey with cryptic behavior, and suggests C. quadricollis and Leptophloeus sp. might be useful biocontrol agents against H. hampei.

  16. DNA barcoding of Japanese click beetles (Coleoptera, Elateridae).

    Science.gov (United States)

    Oba, Yuichi; Ôhira, Hitoo; Murase, Yukio; Moriyama, Akihiko; Kumazawa, Yoshinori

    2015-01-01

    Click beetles (Coleoptera: Elateridae) represent one of the largest groups of beetle insects. Some click beetles in larval form, known as wireworms, are destructive agricultural pests. Morphological identification of click beetles is generally difficult and requires taxonomic expertise. This study reports on the DNA barcoding of Japanese click beetles to enable their rapid and accurate identification. We collected and assembled 762 cytochrome oxidase subunit I barcode sequences from 275 species, which cover approximately 75% of the common species found on the Japanese main island, Honshu. This barcode library also contains 20 out of the 21 potential pest species recorded in Japan. Our analysis shows that most morphologically identified species form distinct phylogenetic clusters separated from each other by large molecular distances. This supports the general usefulness of the DNA barcoding approach for quick and reliable identification of Japanese elaterid species for environmental impact assessment, agricultural pest control, and biodiversity analysis. On the other hand, the taxonomic boundary in dozens of species did not agree with the boundary of barcode index numbers (a criterion for sequence-based species delimitation). These findings urge taxonomic reinvestigation of these mismatched taxa.

  17. The complete mitogenome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae).

    Science.gov (United States)

    Nan, Xiaoning; Wei, Cong; He, Hong

    2016-05-01

    The complete mitochondrial genome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae) were reconstructed from whole-genome Illumina Hiseq 2000 sequencing data with an average coverage of 1406.7X. The circular genome is 15,122 bp in length, consisting of 13 protein-coding genes (PCGs), 21 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and one D-loop or control region. The tRNA-Ile gene was not found in the mitochondrial genome, as is identical to two other curculionidae species, i.e. Sphenophorus sp. (GU176342) and Naupactus xanthographus (GU176345). All PCGs initiated with ATN codons, except for the ND1 started with TTG. Two PCGs (COI and ND4) have an incomplete stop codon T. Two PCGs (ND4L and ND1) harbor the stop codon TAG, while all other PCGs terminated with the TAA codon. The nucleotide composition is highly asymmetric (38.7% A, 14.4% C, 9.2% G and 37.8% T) with an overall AT content of 76.5%. PMID:25427809

  18. Plant compounds insecticide activity against Coleoptera pests of stored products

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Marcio Dionizio; Picanco, Marcelo Coutinho; Guedes, Raul Narciso Carvalho; Campos, Mateus Ribeiro de; Silva, Gerson Adriano; Martins, Julio Claudio [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Biologia Animal]. E-mail: marcio.dionizio@gmail.com; picanco@ufv.br; guedes@ufv.br; mateusc3@yahoo.com.br; agronomiasilva@yahoo.com.br

    2007-07-15

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on R. dominica was evaluated. Among them, only hexane extract of A. conyzoides showed insecticide activity; the hexane extract of this species was successively fractionated by silica gel column chromatography, for isolation and purification of the active compounds. Compounds 5,6,7,8,3',4',5'-heptamethoxyflavone; 5,6,7,8,3'-pentamethoxy-4',5'-methilenedioxyflavone and coumarin were identified. However, only coumarin showed insecticide activity against three insect pests (LD{sub 50} from 2.72 to 39.71 mg g{sup -1} a.i.). The increasing order of insects susceptibility to coumarin was R. dominica, S. zeamais and O. surinamensis. (author)

  19. Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae

    Directory of Open Access Journals (Sweden)

    Elias Soares Gomes

    2014-06-01

    Full Text Available Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae. Coleopterans of the family Melolonthidae comprise a large group of species that feed on different food sources, including plant roots, stems, and leaves, in addition to plant materials at different decomposition stages. Several species are found in the genus Leucothyreus, occurring in different regions of Brazil, including the various biomes in the country. Information on the biology of species of the genus Leucothyreus is scarce, therefore, we conducted studies on the biological aspects of Leucothyreus ambrosius Blanchard, 1850. The period of adult occurrence was determined with a light trap installed between a cropped and pasture area in the municipality of Aquidauana, Mato Grosso do Sul State, Brazil. Adults collected in the field were used to form insect pairs and the studies were initiated in the entomology laboratory as the adults began ovipositing. Adults were observed flying in the field from October to December. Eggs were obtained as pairs were formed and a colony was established, the embryonic period lasting 14.6 days on average. The larval period in the 1st instar lasted 21.6 days, in the 2nd instar 19.6 days, and in the 3rd instar, 85.6 days. The head capsule width was 1.48 mm in the 1st instar, 2.44 mm in the 2nd, and 3.83 mm in 3rd larval instar. The pupal stage had an average duration of 35.5 days. The egg to adult period lasted 173.3 days. Morphometric information for the larval and adult stages is presented in this study.

  20. Using malaise traps to sample ground beetles (Coleoptera. Carabidae)

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Hanula, James L. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Horn, Scott [USDA Forest Service, Savannah River, New Ellenton, SC (United States)

    2012-04-02

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  1. A family of chemoreceptors in Tribolium castaneum (Tenebrionidae: Coleoptera.

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    Mohatmed Abdel-Latief

    Full Text Available Chemoperception in invertebrates is mediated by a family of G-protein-coupled receptors (GPCR. To date nothing is known about the molecular mechanisms of chemoperception in coleopteran species. Recently the genome of Tribolium castaneum was sequenced for use as a model species for the Coleoptera. Using blast searches analyses of the T. castaneum genome with previously predicted amino acid sequences of insect chemoreceptor genes, a putative chemoreceptor family consisting of 62 gustatory receptors (Grs and 26 olfactory receptors (Ors was identified. The receptors have seven transmembrane domains (7TMs and all belong to the GPCR receptor family. The expression of the T. castaneum chemoreceptor genes was investigated using quantification real- time RT-PCR and in situ whole mount RT-PCR analysis in the antennae, mouth parts, and prolegs of the adults and larvae. All of the predicted TcasGrs were expressed in the labium, maxillae, and prolegs of the adults but TcasGr13, 19, 28, 47, 62, 98, and 61 were not expressed in the prolegs. The TcasOrs were localized only in the antennae and not in any of the beetles gustatory organs with one exception; the TcasOr16 (like DmelOr83b, which was localized in the antennae, labium, and prolegs of the beetles. A group of six TcasGrs that presents a lineage with the sugar receptors subfamily in Drosophila melanogaster were localized in the lacinia of the Tribolium larvae. TcasGr1, 3, and 39, presented an ortholog to CO(2 receptors in D. melanogaster and Anopheles gambiae was recorded. Low expression of almost all of the predicted chemoreceptor genes was observed in the head tissues that contain the brains and suboesophageal ganglion (SOG. These findings demonstrate the identification of a chemoreceptor family in Tribolium, which is evolutionarily related to other insect species.

  2. Biocontrol of larval mosquitoes by Acilius sulcatus (Coleoptera: Dytiscidae

    Directory of Open Access Journals (Sweden)

    Banerjee Siddhartha S

    2008-10-01

    Full Text Available Abstract Background Problems associated with resistant mosquitoes and the effects on non-target species by chemicals, evoke a reason to find alternative methods to control mosquitoes, like the use of natural predators. In this regard, aquatic coleopterans have been explored less compared to other insect predators. In the present study, an evaluation of the role of the larvae of Acilius sulcatus Linnaeus 1758 (Coleoptera: Dytiscidae as predator of mosquito immatures was made in the laboratory. Its efficacy under field condition was also determined to emphasize its potential as bio-control agent of mosquitoes. Methods In the laboratory, the predation potential of the larvae of A. sulcatus was assessed using the larvae of Culex quinquefasciatus Say 1823 (Diptera: Culicidae as prey at varying predator and prey densities and available space. Under field conditions, the effectiveness of the larvae of A. sulcatus was evaluated through augmentative release in ten cemented tanks hosting immatures of different mosquito species at varying density. The dip density changes in the mosquito immatures were used as indicator for the effectiveness of A. sulcatus larvae. Results A single larva of A. sulcatus consumed on an average 34 IV instar larvae of Cx. quinquefasciatus in a 24 h period. It was observed that feeding rate of A. sulcatus did not differ between the light-on (6 a.m. – 6 p.m., and dark (6 p.m. – 6 a.m. phases, but decreased with the volume of water i.e., space availability. The prey consumption of the larvae of A. sulcatus differed significantly (P A. sulcatus larvae, while with the withdrawal, a significant increase (p A. sulcatus in regulating mosquito immatures. In the control tanks, mean larval density did not differ (p > 0.05 throughout the study period. Conclusion the larvae of the dytiscid beetle A. sulcatus proved to be an efficient predator of mosquito immatures and may be useful in biocontrol of medically important mosquitoes.

  3. 彩绒革盖菌漆酶产酶条件研究%Research on the Conditions for Laccase Production from Coriolus versicolor

    Institute of Scientific and Technical Information of China (English)

    王宜磊; 刘兴坦

    2001-01-01

    本文研究了碳源、氮源、愈创木酚、香兰素及培养条件对漆酶分泌的影响;结果表明,淀粉作碳源、干酪素作氮源有利于漆酶的分泌,适宜浓度的愈创木酚和香兰素等对漆酶的产生有一定的作用;pH在3.0~8.0的范围内对漆酶的分泌影响差别不大,培养温度、接种量、通气量对漆酶的分泌有较大影响。%Effects of carbon source, nitrogen source, o-methoxyphenol, vanillin and culture conditions on laccase production from Coriolus versicolor were studied. The results showed that starch as carbon source, and casein and soybean powder as nitrogen source, o-methoxyphenol and vanillin of suitable concentration had good influence on laccase production. There were a little difference between effects of pH 3.0~8.0 in the medium on laccase production, but the culture temperature, inoculation amount and ventilation volume had greater influence on laccase production.

  4. A Ca-alginate particle co-immobilized with Phanerochaete chrysosporium cells and the combined cross-linked enzyme aggregates from Trametes versicolor.

    Science.gov (United States)

    Li, Yanchun; Wang, Zhi; Xu, Xudong; Jin, Liqiang

    2015-12-01

    For improving stability of immobilized white-rot fungus to treat various effluents, Phanerochaete chrysosporium cells and the combined cross-link enzyme aggregates (combi-CLEAs) prepared from Trametes versicolor were co-immobilized into the Ca-alginate gel particles in this paper. The activity yields of obtained combi-CLEAs were 42.7% for lignin peroxidases (LiPs), 31.4% for manganese peroxidases (MnPs) and 40.4% for laccase (Lac), respectively. And their specific activities were 30.2U/g as combi-CLEAs-LiPs, 9.5 U/g as combi-CLEAs-MnPs and 28.4 U/g as combi-CLEAs-Lac. Further, the present of the combi-CLEAs in the particles extremely improved their ability to degrade the dyes. Compared to the immobilized Ph. chrysosporium without the combi-CLEAs, the co-immobilized particles enhanced the decolorized rate of Acid Violet 7 (from 45.2% to 93.4%) and Basic Fuchsin (from 12.1% to 67.9%). In addition, the addition of the combi-CLEAs improved the adaptability of the white-rot fungal particles to adverse environmental conditions. PMID:26413897

  5. Gold bugs and beyond: a review of iridescence and structural colour mechanisms in beetles (Coleoptera).

    Science.gov (United States)

    Seago, Ainsley E; Brady, Parrish; Vigneron, Jean-Pol; Schultz, Tom D

    2009-04-01

    Members of the order Coleoptera are sometimes referred to as 'living jewels', in allusion to the strikingly diverse array of iridescence mechanisms and optical effects that have arisen in beetles. A number of novel and sophisticated reflectance mechanisms have been discovered in recent years, including three-dimensional photonic crystals and quasi-ordered coherent scattering arrays. However, the literature on beetle structural coloration is often redundant and lacks synthesis, with little interchange between the entomological and optical research communities. Here, an overview is provided for all iridescence mechanisms observed in Coleoptera. Types of iridescence are illustrated and classified into three mechanistic groups: multilayer reflectors, three-dimensional photonic crystals and diffraction gratings. Taxonomic and phylogenetic distributions are provided, along with discussion of the putative functions and evolutionary pathways by which iridescence has repeatedly arisen in beetles.

  6. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Dilma Solange Napp

    2006-01-01

    Full Text Available Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae. Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae. The following new genus and species, all from Bolivia, are described in Compsocerini: Ecoporanga gen. nov., type species E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., from Santa Cruz, and Ethemon iuba sp. nov. from Cochabamba.

  7. Una especie nueva de Trechisibus de la Argentina (Coleoptera: Carabidae A new species of Trechisibus from Argentina (Coleoptera: Carabidae

    Directory of Open Access Journals (Sweden)

    Sergio Roig-Juñent

    2005-12-01

    Full Text Available RESUMEN. Las Trechini constituyen una tribu de Carabidae distribuida ampliamente en la región Andino Patagónica. A pesar de que existen varias revisiones parciales de esta tribu para la Argentina y Chile, es muy común hallar especies nuevas, debido sobre todo a la prospección de áreas no exploradas. En este aporte se describe una especie nueva del género Trechisibus Jeannel del Cerro Nevado (Mendoza, Argentina. Por sus características morfológicas pertenece al grupo de especies depressus. Se describe e ilustra el adulto de esta especie nueva, se provee una clave para la identificación de las especies del grupo depressus y se discuten algunos aspectos de la distribución de estas especies.ABSTRACT. Trechini is a tribe of Carabidae (Coleoptera widely distributed in the Andean Patagonian region in South America. In spite of several partial revisions of the tribe for Argentina and Chile, it is very common to find new species due to the research of unexplored areas. In the present paper, a new species of the genus Trechisibus Jeannel from the Cerro Nevado (Mendoza, Argentina is described. Based on its morphological features the new species is considered as a member of the depressus group. The adult of the new species is described and illustrated, a key for the identification of the species of the depressus group is provided, and some aspects of the distribution of the group are discussed.

  8. Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil

    OpenAIRE

    Renata C. Campos; Malva I. Medina Hernández

    2013-01-01

    Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil. The beetles of the subfamily Scarabaeinae are important organisms that participate in the cycle of decomposition, especially in tropical ecosystems. Most species feed on feces (dung) or carcasses (carrion) and are associated with animals that produce their food resources. Dung beetles are divided into three functional groups: rollers, tunnelers and dwellers. This present work aims to study the ...

  9. The Leaf-Beetles (Coleoptera, Chrysomelidae) Feed On Some Weeds In Tokat Province

    OpenAIRE

    Çam, Halit; ATAY, Turgut

    2004-01-01

    This study was carried out to determine the leaf-beetles (Coleoptera, Chrysomelidae) which causes extensive damage on weeds in the vicinity of Tokat, Turkey. Total 9 taxa including 4 species of Chrysomelinae, 1 species of Clytrinae, 1 species of Criocerinae, 2 species of Alticinae and 1 species of Cassidinae were found to be destructive on different weed species. These species were; Entomoscelis adonidis (Pall.) on Sinapis arvensis L., Gastrophysa polygoni (L.) on Polygonum convolvulus L. an...

  10. Species of beetles (Coleoptera; Scarabaeidae associated to banana (Musa spp. in Ceballos, Ciego de Avila, Cuba

    Directory of Open Access Journals (Sweden)

    Maria Luisa Sisne Luis

    2016-03-01

    Full Text Available A white light trap was placed in bananas plantations, according to Sisne, 2009 and MINAG, 1985, in the Citric enterprise of Ciego de Ávila during the period between May and July of 2010 with the objective of determining the composition of genus and species of the order Coleoptera family Scarabaeidae associated to the agroecosystem. The species Cyclocephala cubana Chapin, Phyllophaga puberula Duval, and Phyllophaga patruelis Chev. are associated to bananas crops in these areas.

  11. Ecological impact of entomopathogenic nematodes used to control the large pine weevil, Hylobius abietis (Coleoptera: Curculionidae)

    OpenAIRE

    Harvey, Christopher D.

    2010-01-01

    The large pine weevil (Hylobius abietis; Coleoptera: Curculionidae) is one of the most economically damaging pests in forestry across Northern Europe. Its larvae develop in coniferous tree stumps on clearfell sites and adult weevils feed on seedlings that are replanted on these sites, causing substantial mortality. As the drive towards achieving the sustainable management of natural resources increases, biological control agents are being considered as an alternative to chemical ...

  12. Otiorhynchus spp. (Coleoptera: Curculionidae) as pests in horticulture: genetics and management options with entomopathogenic fungi

    OpenAIRE

    Hirsch, Jacqueline

    2012-01-01

    Worldwide, weevils of the genus Otiorhynchus (Coleoptera: Curculionidae) cause damage with detrimental economic effects to many horticultural crops due to the root feeding of their larvae as well as foliage feeding of their adults. Aside from the black vine weevil Otiorhynchus sulcatus, which is the best-known pest within this genus, numerous other Otiorhynchus species have been increasingly recognized as pests in recent years. Nocturnal adult weevils and soil-inhibiting larvae are in princip...

  13. Laboratory Rearing of Laricobius nigrinus (Coleoptera: Derodontidae): A Predator of the Hemlock Woolly Adelgid (Hemiptera: Adelgidae)

    OpenAIRE

    Salom, S. M.; L. T. Kok; Lamb, A B; Jubb, C

    2012-01-01

    Coleopteran species are biological control agents of numerous invasive pests. Laricobius nigrinus (Coleoptera: Derodontidae), a predaceous, univoltine species, spends the summer aestivating but is active for the rest of the year. Laricobius nigrinus possesses many essential attributes for effective biological control of the hemlock woolly adelgid (Hemiptera: Adelgidae). The predator must be reared in large numbers for field releases. We describe some of the studies that led to the successful ...

  14. Life History of the Tamarind Weevil, Sitophilus linearis (Herbst) (Coleoptera: Curculionidae), on Tamarind Seed

    OpenAIRE

    James Adebayo Ojo; Adebayo Amos Omoloye

    2015-01-01

    The tamarind weevil, Sitophilus linearis Herbst (Coleoptera: Curculionidae), is an important pest of tamarind and other Caesalpinioideae. Investigating its life history is important in the implementation of management strategy. Its life history was monitored daily to understand its developmental biology on tamarind seed following standard procedures under laboratory conditions of 24–30°C temperature, 60–70% relative humidity, and 12L : 12D photoperiod. The egg incubation period lasted 3.17 ± ...

  15. New national and state records of Neotropical Staphylinidae (Insecta: Coleoptera).

    Science.gov (United States)

    Asiain, Julieta; Márquez, Juan; Irmler, Urlich

    2015-01-01

    Eighteen new national records of nine species of Osoriinae (Staphylinidae) are added for 10 Neotropical countries. Additionally, 17 species of three subfamilies are first recorded from ten States of México. The distributional patterns of the studied species are commented and the congruence with species of different families of Coleoptera and Odonata previously analyzed is discussed. Finally, we conclude that some of these patterns can be proposed as hypothesis of primary biogeographic homology. PMID:26249885

  16. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern

    Directory of Open Access Journals (Sweden)

    SUGIYARTO

    2009-07-01

    Full Text Available Maryati KT, Sugiyarto. 2010. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern. Nusantara Bioscience 1: 72-77. This research aims to find out the white grub (Melolonthidae; Coleoptera variability based on the morphological characteristic and protein banding pattern found in ”salak pondoh” farm in Regencies of Sleman, Yogyakarta and Magelang, Central Java. Each area has five sampling points. Morphological analysis on white grub was conducted using descriptive method and analysis on protein banding pattern was conducted using qualitative analysis based on the presence or absent of band pattern on the gel, and qualitatively based on the relative mobility value (Rf of protein. The result indicated that the white grub in Sleman and Magelang, based on morphology characteristic is only one species, namely Holothricia sp. Based on the protein banding pattern, the white grub sample have differences of protein band number and protein molecular weight. Key words: Salacca zalacca, white grub, morphology, protein banding pattern.Abstrak. Maryati KT, Sugiyarto. 2010. Karakterisasi lundi putih (Melolonthidae: Coleoptera pada pertanaman salak berdasarkan ciri morfologi dan pola pita protein. Nusantara Bioscience 1: 72-77. Penelitian ini bertujuan untuk mengetahui keanekaragaman lundi putih (Melolonthidae; Coleoptera berdasarkan ciri morfologi dan pola pita protein yang ditemukan di lahan pertanaman salak pondoh di Kabupaten Sleman, Yogyakarta dan Kabupaten Magelang, Jawa Tengah. Pada masing-masing wilayah diambil lima titik sampling. Analisis morfologi lundi putih digunakan metode deskriptif, dan analisis pola pita protein digunakan analisis kualitatif berdasarkan muncul tidaknya pola pita pada gel, dan secara kuantitatif berdasarkan nilai mobilitas relatif protein (RF. Hasil penelitian menunjukkan bahwa sampel lundi putih di Kabupaten Sleman dan Magelang, berdasar karakter

  17. Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil

    OpenAIRE

    Andreia Mauruto Chernaki-Leffer; Daniel Ricardo Sosa-Gómez; Lúcia M. de Almeida; Ivani de Oliveira Negrão Lopes

    2011-01-01

    Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer), is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvo...

  18. Novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae da Bolívia

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    Ubirajara R. Martins

    2008-01-01

    Full Text Available Novas espécies descritas da Bolívia, Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.New species of Onciderini (Coleoptera, Cerambycidae, Lamiinae from Bolivia. New species described from Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.

  19. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae)

    OpenAIRE

    M.M. Kiradoo; Meera Srivastava

    2011-01-01

    The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae) is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insect...

  20. NEW CONTRIBUTION CONCERNING THE MASS REARING OF TANYMECUS DILATICOLLIS GYLL (COLEOPTERA: CURCULIONIDAE) UNDER CONTROLLED CONDITIONS

    OpenAIRE

    MANOLE Traian; Irina IONESCU-MĂLĂNCUŞ; Iuliana ANTONIE; Laurenţiu Constantin REBEGA

    2013-01-01

    Controlled growth in the laboratory of some species of insect’s are justified both scientifically and practically. Due to the economic importance that the corn crop pest Tanymecus dilaticollis Gyll. (Coleoptera: Curculionidae) had in Romania like the main pest of maize crops we tried to obtain successive generations under environment controlled of the insect mentioned. In this paper we aim to improve the efficiency of the multiplication of the species with respect to two aspects: attending la...

  1. DIVERSITY OF CARABIDS (COLEOPTERA) ASSOCIATED WITH THE LOWER LURIN RIVER, LIMA, PERU

    OpenAIRE

    ARMANDO VÉLEZ-AZAÑERO; Alfonso Lizárraga-Travaglini

    2013-01-01

    Quarterly samples of beetles (Coleoptera: Carabidae) were taken associated with the lower basin of the Lurín River (Lima-Peru) during the period August 2009 - February 2011, in six sampling points between 5 and 51 masl. Pitfall traps were used and obtained a total of 59 specimens distributed among three tribes, three genera, and four morphospecies. We report the presence of Megacephala, Scarites genus, and Pterostichus with the latter being the predominant genus.

  2. Incorporating a Sorghum Habitat for Enhancing Lady Beetles (Coleoptera: Coccinellidae) in Cotton

    OpenAIRE

    P. G. Tillman; Cottrell, T. E.

    2012-01-01

    Lady beetles (Coleoptera: Coccinellidae) prey on insect pests in cotton. The objective of this 2 yr on-farm study was to document the impact of a grain sorghum trap crop on the density of Coccinellidae on nearby cotton. Scymnus spp., Coccinella septempunctata (L.), Hippodamia convergens Guérin-Méneville, Harmonia axyridis (Pallas), Coleomegilla maculata (De Geer), Cycloneda munda (Say), and Olla v-nigrum (Mulsant) were found in sorghum over both years. Lady beetle compositions in sorghum and ...

  3. Biological activities of Allium sativum essential oil against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae)

    OpenAIRE

    Chaubey Mukesh Kumar

    2014-01-01

    Essential oil from Allium sativum was isolated and investigated for its repellent, insecticidal, ovipositional and egg hatching inhibition activities against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae). A. sativum essential oil repelled bruchid adults at a very low concentration in choice oviposition assay. A. sativum essential oil caused both fumigant and contact toxicity in C. chinensis adults in a concentration dependent manner. Oviposition potency of C. chinensis adults...

  4. Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)

    Science.gov (United States)

    Nenadić, Marija; Soković, Marina; Glamočlija, Jasmina; Ćirić, Ana; Perić-Mataruga, Vesna; Ilijin, Larisa; Tešević, Vele; Vujisić, Ljubodrag; Todosijević, Marina; Vesović, Nikola; Ćurčić, Srećko

    2016-04-01

    The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens—eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var . cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var . cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.

  5. 杂色龙虾线粒体基因组全序列的测定与分析%Sequencing and analysis of the complete mitochondrial geno-me of Panulirus versicolor

    Institute of Scientific and Technical Information of China (English)

    刘皓; 姚维; 刘海情; 刘楚吾; 刘丽

    2014-01-01

    为了解杂色龙虾(Panulirus versicolor)线粒体基因组多态位点及遗传变异,对龙虾类的分类研究奠定基础,作者通过常规 PCR步移扩增法获得杂色龙虾线的线粒体基因组全序列,分析了其线粒体基因组的基本特征以及基因排列情况,并结合中国龙虾(Panulirus stimposni)、波纹龙虾(Panulirus homa-rus)线粒体基因组全序列,综合分析了龙虾属线粒体编码基因多态位点及基因变异状况。结果表明:杂色龙虾线粒体基因组全序列为15700 bp,由13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和 D-loop区组成,保持了泛甲壳动物线粒体基因组的原始排列。杂色龙虾线粒体 DNA 的碱基组成具有AT偏好性, A+T含量为67%,4种碱基的含量分别为(A=34.6%, T=32.1%, C=20.5%, G=12.8%)。预测了杂色龙虾18个tRNA以及2个rRNA的二级结构。龙虾属线粒体基因组中的nad 2、nad 5基因以及D-loop 区的多态位点比例较高,适合作为分子标记用于龙虾类系统进化关系、种内多态性以及遗传分化等方面的研究。%The complete sequence of Panulirus versicolor mitochondrial genome was determined by using conventional PCR and conserved primers walking approaches. The basic characteristics and gene arrangement of P. versicolor mito-chondrial genome were analyzed. Combined with the mitochondrial genome of P. stimposni and P. homarus, the mito-chondrial genes encoding polymorphic loci and gene mutation of Panulirus were also analyzed. The results show that the entire mitochondrial genome of P. versicolor is 15700 bp in length, consisting of 13 protein-coding genes, 22 tRNA genes and 2 rRNA genes and D-loop control area, which maintained the original arrangement of crustacean animal mito-chondrial genomes. The composition of P. versicolor mitochondrial DNA base has AT preference, with 67%A+T con-tent and the content of A, T, C, and G was 34.6%, 32.1%, 20.5,%and 12.8%respectively. The secondary

  6. Alien seed beetles (Coleoptera: Chrysomelidae: Bruchinae) in Europe.

    Science.gov (United States)

    Yus-Ramos, Rafael; Ventura, Daniel; Bensusan, Keith; Coello-García, Pedro; György, Zoltán; Stojanova, Anelia

    2014-07-01

    Under the framework of the DAISIE consortium, whose main mission is to make an inventory of the alien invasive species of Europe and its islands, we review the current state of knowledge and provide an up-to-date catalogue and distributional status for alien seed beetles (Coleoptera: Chrysomelidae: Bruchinae) in Europe. This work is based on studies of the species detected from the last century to the present, but with greater emphasis on the beginning of the 21st century, during which new biological studies have been carried out and findings made in European countries. The main objective of this paper is to focus on this last fact, which has promoted new views on the existing and potential threat of exotic bruchids in relation to climate change. This must now be regarded as a matter of concern for European agricultural and environmental policies. Only species of exotic origin introduced in European regions outside their native range were considered. Therefore, species of European origin spreading to new countries within Europe are not treated. Also, we provide a new approach to classifying alien seed beetle species according to their ability to become established, distinguishing between the well-established and those that may appear in seed stores but are not capable of invading natural and agricultural ecosystems. We present a taxonomic characterization of the alien bruchids found in Europe, providing an illustrated key based on external morphological characters of adults. The key facilitates the identification of the sixteen most frequently recorded genera, which represent 37 of the 42 species of exotic species recorded in Europe up to the present, whether established, not established or occasional. Finally, we provide a summary of the state of knowledge of the taxonomy and biology of the 20 most worrying species as pests, both established and non-established. This includes, where appropriate, an illustrated key for the identification of species. The study

  7. Summarization of Researches on Chemical Compositions and Pharmacological Activities of Coriolus versicolor%云芝的化学成分及药理活性研究概况

    Institute of Scientific and Technical Information of China (English)

    李玲艳; 包海鹰

    2011-01-01

    The article has carried on the summarization of chemical compositions and pharmacological activities of Coriolus versicolor through document retrieving.Present studies have found that Coriolus versicolor contains 29 compounds,such as steroids,triterpenes,organic acids,alkaloids,Glucitols,proteins,carbohydrates,glycopeptides.Scholars has also found Coriolus versicolor contains many other compounds:ECMM,pepsin inhibitor,Elicitor,iron,conglutinated chelating agents,cyclopeptides,18 kinds of essential amino acids,VB1,VB2,VB6 and 10 more human body′s essential activitied microelements.Coriolus versicolor has such pharmacological actions as anti-tumor,analgesia,treating Chronic Hepatitis B,norblood fat,antiatherosclerosis,antioxi-dation,antiaging,benefiting vital energy and antipyresis,antimicrobial activities in vitro.%通过文献检索对云芝的化学成分及药理活性方面进行了综述。研究发现云芝含甾体类、三萜类、有机酸类、生物碱类、葡糖醇类、蛋白质类、糖类、糖肽类等29个化合物,还含胞外黏性物质(ECMM)、胃蛋白酶抑制剂、真菌激发子、铁黏合螯合剂、环肽、18种人体所需的氨基酸和维生素B1、B2、B6及铜、铁、钾、锌等10多种人体所需的活性微量元素等其他类化合物。云芝具有抗肿瘤、镇痛、治疗慢性乙型肝炎、降血脂和抗动脉粥样硬化、抗氧化、抗衰老、益气解毒、体外抗微生物活性等作用。

  8. Comparative Growth and Survival of Hylurgus ligniperda (Coleoptera: Scolytinae) and Arhopalus ferus (Coleoptera: Cerambycidae) Reared on Artificial or Natural Diet at 15 or 25°C.

    Science.gov (United States)

    Romo, C M; Bader, M K-F; Pawson, S M

    2016-02-01

    Two saproxylic forest insects, Hylurgus ligniperda (F.) (Coleoptera: Scolytinae) and Arhopalus ferus (Mulsant)(Coleoptera: Cerambycidae), were reared on artificial or natural diet at 15 or 25°C to compare larval growth rates and survival. A significant diet by temperature interaction was observed in the growth of H. ligniperda larvae,which developed faster when reared on natural diet at 15°C, but grew faster and pupated significantly earlier when reared on artificial diet at 25°C. However, H. ligniperda survival by the end of the experiment was low on both diets when reared at 25°C (10.1%, 95% CI: 5.2–15.1%), which suggests that rearing at lower temperatures may be required. A. ferus larvae gained significantly larger body size when reared on artificial diet than on natural diet at both temperatures. Survival of A. ferus reared on artificial diet was significantly lower than larvae reared on natural diet at 25°C. The significant differences between A. ferus larval development rates when reared on artificial and natural diets preclude the use of artificial diet to collect meaningful data to construct temperature development models for ecological comparisons. Artificial diet provided a suitable medium for mass production of individuals for research purposes, e.g., test mortality in response to treatments. However, additional rearing studies are needed to determine whether the larger artificially reared larvae result in adults that are healthier, more productive, and live longer.

  9. Especies mexicanas de Curculionidae (Insecta: Coleoptera asociadas con agaves (Asparagaceae: Agavoideae Mexican species of Curculionidae (Insecta: Coleoptera associated to agaves (Asparagaceae: Agavoideae

    Directory of Open Access Journals (Sweden)

    Alberto Romo

    2012-12-01

    Full Text Available Se estudiaron las especies de picudos o gorgojos (Coleoptera: Curculionidae asociadas con agaves (Asparagaceae: Agavoideae en México. Se registraron 5 especies asociadas con especies de Agave, Furcraea, Hesperoyucca, Polianthes y Yucca; de éstas, 4 pertenecen a la subfamilia Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis y Cactophagus spinolae y 1 a la Baridinae (Peltophorus polymitus. Se presentan diagnosis, ilustraciones y una clave para la identificación de las 5 especies de curculiónidos asociados con agaves. Se establecen las siguientes sinonimias: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; y Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891 y Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.Weevils (Coleoptera: Curculionidae associated with agaves (Asparagaceae: Agavoideae from Mexico are analyzed. Five species were recorded associated with species of Agave, Furcraea, Hesperoyucca, Polianthes, and Yucca. Four of these species belong to the subfamily Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis, and Cactophagus spinolae and 1 belongs to the subfamily Baridinae (Peltophorus polymitus. Diagnoses, illustrations and a key are presented for identifying the 5 species of weevils found on agaves. The following synonymies are established: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; and Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891, and Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.

  10. Evaluation of Standard Loose Plastic Packaging for the Management of Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebriondiae)

    Science.gov (United States)

    Hassan, Muhammad Waqar; Gulraize; Ali, Usman; Ur Rehman, Fazal; Najeeb, Hafsa; Sohail, Maryam; Irsa, Bakhtawar; Muzaffar, Zubaria; Chaudhry, Muhammad Shafiq

    2016-01-01

    Three standard foodstuff plastic packaging namely polyethylene (PE), polypropylene (PP), and polyvinylchloride (PVC) were evaluated for management of lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Resistance parameters in packaging were recorded as punctures, holes, penetrations, sealing defects, and invasions with two thicknesses and tested for two lengths of time. Damages like punctures, holes and penetrations by both insects were more in PE packaging however R. dominica made more penetrations in PP than in PE. For both insects sealing defects and invasions were predominant in PVC than in others. Thickness did not affect significantly damage types but significantly more holes and penetrations by R. dominica were in less thickness. Punctures and holes by R. dominica were more after less time period but other damages in packaging were more after more time period. However for T. castaneum all sorts of damages were seen more after more time period. Overall categorization between two insects showed R. dominica made more penetrations and T. castaneum made more invasions compared with their counterparts. Pictures were taken under camera fitted microscope to magnify punctures and holes in different packaging and thicknesses. Insect mortality due to phosphine was more in PP and PE packaging and least in PVC packaging and thickness effect was marginal. T. castaneum mortality was significantly more after 48 h than after 24 h. Damages extent in packaging and fumigation results showed PP to be the best of three packaging materials to manage these insects. PMID:27638958

  11. Evaluation of Standard Loose Plastic Packaging for the Management of Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebriondiae).

    Science.gov (United States)

    Hassan, Muhammad Waqar; Gulraize; Ali, Usman; Ur Rehman, Fazal; Najeeb, Hafsa; Sohail, Maryam; Irsa, Bakhtawar; Muzaffar, Zubaria; Chaudhry, Muhammad Shafiq

    2016-01-01

    Three standard foodstuff plastic packaging namely polyethylene (PE), polypropylene (PP), and polyvinylchloride (PVC) were evaluated for management of lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Resistance parameters in packaging were recorded as punctures, holes, penetrations, sealing defects, and invasions with two thicknesses and tested for two lengths of time. Damages like punctures, holes and penetrations by both insects were more in PE packaging however R. dominica made more penetrations in PP than in PE. For both insects sealing defects and invasions were predominant in PVC than in others. Thickness did not affect significantly damage types but significantly more holes and penetrations by R. dominica were in less thickness. Punctures and holes by R. dominica were more after less time period but other damages in packaging were more after more time period. However for T. castaneum all sorts of damages were seen more after more time period. Overall categorization between two insects showed R. dominica made more penetrations and T. castaneum made more invasions compared with their counterparts. Pictures were taken under camera fitted microscope to magnify punctures and holes in different packaging and thicknesses. Insect mortality due to phosphine was more in PP and PE packaging and least in PVC packaging and thickness effect was marginal. T. castaneum mortality was significantly more after 48 h than after 24 h. Damages extent in packaging and fumigation results showed PP to be the best of three packaging materials to manage these insects. PMID:27638958

  12. 云芝漆酶对靛蓝废水脱色降解研究%Decolorization on Indigo Dyeing Wastewater by Laccase from Coriolus versicolor

    Institute of Scientific and Technical Information of China (English)

    高恩丽; 仲伟佳; 付小兰; 陈福山; 叶志刚

    2012-01-01

    [Object] The study aimed to discuss the decolorization on indigo dyeing wastewater by laccase from Coriolus versicolor. [ Method] Firstly, the effects of temperature, pH, indigo concentration, HBT concentration, laccase dosage on the decolorization of indigo dyeing wastewater by laccase/HBT, and then the synergism of laccase and acid cellulase was analyzed. [Result] Using ABTS as the substrate, the kinetic parameters, Km and Vm, were 0. 318 mmol/L and 0.035 5 mmol/( L · min) respectively. The decolorization rate of indigo reached 96.5% with HBT as introducer for40 min at temperature 50 °C, pH=4.5, indigo concentration 100 mg/L, HBT concentration 0. 1% and laccase dosage 100 IU/L. Reducing backstaining of blue jeans by the synergism of laccase and acid cellulase during the bio-finishing was experimented, and the results showed that the backstaining degree of blue jeans was reduced by 85% when the amount of laccase was 15 000 IU/kg. Besides, the synergism of laccase and acid cellulase decreased the amount of indigo in wastewater by 83. 8% . [ Conclusion] The laccase from Coriolus versicolor had a good prospect in the bio-finishing of blue jeans and the decolorization of indigo dyeing wastewater.%[目的]探讨云芝漆酶对靛蓝废水的脱色净化作用.[方法]首先研究不同温度、pH、靛蓝浓度、HBT添加量、漆酶用量对漆酶/HBT脱色作用的影响,然后分析漆酶和酸性纤维素的酶协同水洗.[结果]当以1-羟基苯并三唑(简称HBT)为介体时,云芝漆酶/HBT对靛蓝作用的动力学参数Km和Vmax分别为0.318 mmol/L和0.035 5 mmol/(L·min).在温度50℃,pH4.5,靛蓝浓度100 mg/L,介体HBT用量0.1%,漆酶用量100 IU/L条件下,作用40 min时,靛蓝脱色率高达96.5%.牛仔布生物整理中采用漆酶与酸性纤维素酶协同作用,当漆酶用量为15 000IU/kg牛仔布时,牛仔布的返沾程度减少85%,同时水洗废水中靛蓝含量减少83.8%.[结论]漆酶在牛仔服的生物整理及染整

  13. Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT on human leukemia HL-60 cells

    Directory of Open Access Journals (Sweden)

    Jiang Pingping

    2010-04-01

    Full Text Available Abstract Background Polysaccharopeptide (PSP from Coriolus versicolor (Yunzhi is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT. Methods We used bivariate bromodeoxyuridine/propidium iodide (BrdUrd/PI flow cytometry analysis to measure the relative movement (RM of the BrdUrd positively labeled cells and DNA synthesis time (Ts on the HL-60 cell line. We used annexin V/PI flow cytometry analysis to quantify the viable, necrotic and apoptotic cells. The expression of cyclin E and cyclin B1 was determined with annexin V/PI flow cytometry and western blotting. Human peripheral blood mononuclear cells were used to test the cytotoxicity of PSP and CPT. Results PSP reduced cellular proliferation; inhibited cells progression through both S and G2 phase, reduced 3H-thymidine uptake and prolonged DNA synthesis time (Ts in HL-60 cells. PSP-pretreated cells enhanced the cytotoxicity of CPT. The sensitivity of cells to the cytotoxic effects of CPT was seen to be the highest in the S-phase and to a small extent of the G2 phase of the cell cycle. On the other hand, no cell death (measured by annexin V/PI was evident with the normal human peripheral blood mononuclear cells with treatment of either PSP or CPT. Conclusion The present study shows that PSP increases the sensitization of the HL-60 cells to undergo effective apoptotic cell death induced by CPT. The pattern of sensitivity of cancer cells is similar to that of HL-60 cells. PSP rapidly arrests and/or kills cells in S-phase and did not interfere with the anticancer action of CPT. PSP is a potential adjuvant to treat human leukemia as rapidly proliferating tumors is characterized by a high proportion of S-phase cells.

  14. Role of Malassezia in the color diversity of pityriasis versicolor%马拉色菌在花斑癣色素改变中的作用

    Institute of Scientific and Technical Information of China (English)

    崔凡; 余晓东; 李筱芳; 陈先进; 沈永年; 吕桂霞; 刘维达

    2008-01-01

    Objective To investigate the influence of Malassezia isolates that can cause hyperpigmentation or hypopigmentation in patients with pityriasis versicolor on the melanogenesis of B16F10 melanoma cells.and to detect the levels of cytokines associated with melanogenesis.Methods Primary culture of keratinocytes(1×106 cells/mL)derived from human prepuce were co-cultured with Malassezia strains isolated from hyperpigmented or hypopigmented lesions in the same patient with pityriasis versicolor,with the concentration ratio of keratinocytes to Malassezia cells being 1:1,1:10,1:20 and 1:30,respectively.The proliferation rate of keratinocytes was detected by MTT methOd.To observe the influence on B16F10 melanoma cells.five difierent types of culture supematant were prepared,i.e.the supematant from culture system of keratinocytes alone,supematant from the coculture system of keratinocytes and Malassezia isolates from hyperpigmented sites or hypopigmented sites,and supernatant from keratinocyte culture in the presence of Malassezia cell-free supematant from the culture medium of Malassezia strains from hyperpigmented or hypopigmented sites.ELISA test was performed in these supematants to detect the levels of several eytokines associated with melanogenesis,including basic fibroblast growth factor(b-FGF),endothelin-1,nerve growth factor(NGF)-β,IL-1α,lL-6,tumor necrosis factor(TNF)-α and stem cell factor(SCF).B16F10 cells were cultured with the above 5 supematants.After 48-hour culture.the proliferation rate of B16F10 cells was measured with MTT method,melanin content by NaOH assay.expression and activity of tyrosinase via Western blot and dopa-oxidase assay,respectively.Results The growth rate of keratinocytes was not affected by MalassczJa(P>0.05)when the concentration ratio of keratinocytes to Malassezia cells was more than 1:20.The melanin content,tyrosinase activity and expression of tyrosinase of B16F10 cells were inereased in the presence of supernatant from co

  15. New myrmecomorphous longhorned beetles from Haiti and the Dominican Republic with a key to Anaglyptini and Tillomorphini of Hispaniola (Coleoptera: Cerambycidae: Cerambycinae)

    Science.gov (United States)

    First records of the tribes Anaglyptini and Tillomorphini (Coleoptera: Cerambycidae: Cerambycinae) are documented for Hispaniola. A new genus of highly myrmecomorphic longhorned beetle (Licracantha, new genus) is described and illustrated based on one species (Licracantha formicaria, new species) a...

  16. The influence of vegetation and landscape structural connectivity on butterflies (Lepidoptera: Papilionoidea and Hesperiidae), Carabids (Coleoptera: Carabidae), Syrphids (Diptera: Syrphidae), and sawflies (Hymenoptera: Symphyta) in Northern Italy farmland

    NARCIS (Netherlands)

    Burgio, G.; Sommaggio, D.; Marini, M.; Chiarucci, A.; Landi, S.; Fabbri, R.; Pesarini, F.; Genghini, M.; Ferrari, R.; Muzzi, E.; Lenteren, van J.C.; Masetti, A.

    2015-01-01

    Landscape structure as well as local vegetation influence biodiversity in agroecosystems. A study was performed to evaluate the effect of floristic diversity, vegetation patterns, and landscape structural connectivity on butterflies (Lepidoptera: Papilionoidea and Hesperiidae), carabids (Coleoptera:

  17. Behavioral responses of plum curculio (Coleoptera: Curculionidae) to different enantiomer concentrations and blends of the synthetic aggregation pheromone grandisoic acid

    Science.gov (United States)

    Host plant odors are important for insect location of food and mates. Synergy between host plant odors and aggregation pheromones occurs in many Curculionidae species. The plum curculio Conotrachelus nenuphar Herbst (Coleoptera: Curculionidae) is a major pest of pome and stone fruit. Males produce t...

  18. Nuevos datos de distribución de los Cholevinae hipogeos del Atlas marroquí (Coleoptera, Leiodidae

    Directory of Open Access Journals (Sweden)

    Fresneda, J.

    2006-12-01

    Full Text Available New distribution data for the hypogean Cholevinae from the Moroccan Atlas (Coleoptera, Leiodidae The authors report new findings on the distribution of Speonemadus maroccanus (Jeannel, 1936, Nargus (Demochrus rufipennis (Lucas, 1846, Choleva (Choleva kocheri Henrot, 1962 and Catops fuscus fuscoides Reitter, 1909. The geonemy of these species is updated and the research is illustrated with maps of their distribution.

  19. Release and distribution of Lilioceris cheni (Coleoptera: Chrysomelidae), a biological control agent of air potato (Dioscorea bulbilfera: Dioscoreaceae), in Florida

    Science.gov (United States)

    From 2012 to 2015, 429,668 Lilioceris cheni Gressit and Kimoto (Coleoptera: Chrysomelidae) were released in Florida for biological control of air potato [Dioscorea bulbilfera L. (Dioscoreaceae)]. The spatial distribution of releases was highly aggregated, with several areas of high density releases ...

  20. A NOVEL CADHERIN-LIKE GENE FROM WESTERN CORN ROOTWORM, DIABROTICA VIRGIFERA VIRGIFERA (COLEOPTERA: CHRYSOMELIDAE), LARVAL MIDGUT TISSUE

    Science.gov (United States)

    A cadherin-like gene and its mRNA were cloned from western corn rootworm (Diabrotica virgifera virgifera: Coleoptera), an economically important agricultural pest in North America and Europe. The full length cDNA (5371 bp in length) encodes an open reading frame for a 1688 amino ...

  1. Development and characterization of 11 microsatellite markers in the root-gall-forming weevil, Ceutorhynchus assimilis (Coleoptera: Curculionidae)

    Science.gov (United States)

    The host race of Ceutorhynchus assimilis (Coleoptera: Curculionidae) that specifically develops on Lepidium draba (Brassicales: Brassicaceae), an invasive weed in North America, is being considered for use as a biocontrol agent. Because there are other races that attack other plants, it is important...

  2. Sorghum halepense (L.) Persoon (Poaceae), a new larval host for the South American corn rootworm Diabrotica speciosa (Germar) (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Diabrotica speciosa is a South American corn rootworm (Coleoptera: Chrysomelidae: Galerucinae) the adult of which is a pest on many crop and ornamental plants. The list of known larval hosts, however, is limited to maize, wheat, potatoes and peanuts. In March, 2005, larvae of D. speciosa were found ...

  3. Seasonal flight activity and distribution of metallic woodboring beetles (Coleoptera: Buprestidae) collected in North Carolina and Tennessee

    Science.gov (United States)

    Metallic wood boring insects (Coleoptera: Buprestidae) species are responsible for high levels of host plant injury to deciduous shade and flowering trees in commercial nurseries, urban forests, and managed landscapes. Ornamental plant producers in the southeastern U.S. have ranked borers, includin...

  4. Acoustic detection of Oryctes rhinoceros (Coleoptera: Scarabaeidae: Dynastinae) and Nasutitermes luzonicus (Isoptera: Termitidae) in palm trees of urban Guam

    Science.gov (United States)

    Adult and larval Oryctes rhinoceros (L) (Coleoptera: Scarabaeidae: Dynastinae) were acoustically detected in live and dead palm trees and logs in recently invaded areas of Guam, along with Nasutitermes (Isoptera: Termitidae), and other small, sound-producing invertebrates and invertebrates. The sou...

  5. Records of unsuccessful attack by Anoplophora glabripennis (Coleoptera: Cerambycidae) on broadleaf trees of questionable suitability in Ontario

    Science.gov (United States)

    Discovery of the non-native Anoplophora glabripennis Motschulsky (Coleoptera: Cerambycidae) in Ontario, Canada, in 2003 led to the implementation of an eradication program. The plan consisted of removing all infested trees and all trees within 400 m of an infested tree belonging to a genus consider...

  6. First record of the agave snout weevil, Scyphophorus acupunctatusGyllenhal (Coleoptera: Curculionidae: Dryophthorinae), in Puerto Rico

    OpenAIRE

    Setliff, Gregory P.; Anderson, Jesse A.

    2011-01-01

    The agave snout weevil, Scyphophorus acupunctatus Gyllenhal (Coleoptera: Curculionidae: Dryophthorinae), is reported from Puerto Rico for the first time. It was collected on feral sisal, Agave sisalana Perrine (Agavaceae), in the Guánica Dry Forest Reserve in the southwestern part of the island.

  7. Redescrição e transferência do gênero Fregolia Gounelle, 1911 para Callidiopini (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Jose Ricardo M. Mermudes

    2002-01-01

    Full Text Available Redescription and transference of the genus Fregolia Gounelle, 1911 to Callidiopini (Coleoptera, Cerambycidae. The genus Fregolia is transferred from Cleomenini Lacordaire, 1869 to Callidiopini Lacordaire, 1869. The genus and its type species, Fregolia listropteroides Gounelle, 1911, the only known species to the genus, are redescribed including characters of the mouth pieces, endosternites, wing venation, and male and female terminalia.

  8. Resistance in Cultivated Sunflower Germplasm to the Red Sunflower Seed Weevil (Coleoptera: Curculionidae) in the Northern Great Plains

    Science.gov (United States)

    A 6-year field study evaluated 52 sunflower, Helianthus annuus L., accessions, 20 breeding lines, and 9 interspecific crosses for resistance to infestation by naturally occurring populations of the red sunflower seed weevil, Smicronyx fulvus LeConte (Coleoptera: Curculionidae). Germplasm with potent...

  9. Het lieveheersbeestje Harmonia axyridis in Nederland: een aanwinst voor onze fauna of een ongewenste indringer (Coleoptera: Coccinellidae)?

    NARCIS (Netherlands)

    Cuppen, J.; Heijerman, Th.; Wielink, van P.; Loomans, A.

    2004-01-01

    Harmonia axyridis in the Netherlands: a gain for the fauna or an unwanted intruder (Coleoptera: Coccinellidae)? The coccinellid Harmonia axyridis (Pallas, 1773), a well-known aphid predator originating from Asia, was found for the first time in the Netherlands (Heilige Landstichting near Nijmegen) i

  10. Involvement of larder beetles (Coleoptera: Dermestidae) on human cadavers: a review of 81 forensic cases.

    Science.gov (United States)

    Charabidze, Damien; Colard, Thomas; Vincent, Benoit; Pasquerault, Thierry; Hedouin, Valery

    2014-11-01

    From 1994 to 2013, French forensic entomology laboratories investigated 1,093 cases. Larder beetles (Coleoptera: Dermestidae) were observed in 81 (7.5%) of these cases. To describe and analyze these 81 cases, eight parameters were used: city, location (indoor or outdoor), decay stage (fresh, decay, or dry), dermestid species and instar (adults and/or larvae), presence of living calliphorid larvae, presence of calliphorid pupae or adults, and presence of other necrophagous species. Eight Dermestidae species were observed: Dermestes frischii (42% of cases), Dermestes undulatus (35.8%), Dermestes peruvianus (12.3%), Dermestes lardarius (9.9%), Dermestes haemorrhoidalis (8.6%), Dermestes maculatus (7.4%), Dermestes bicolor (3.7%), and Dermestes ater (1.2%). Larder beetles primarily developed on human cadavers in outdoor locations in areas with a dry climate and were never reported in oceanic areas (which are characterized by frequent rainfall and high ambient humidity). The number of dermestid species on a single corpse never exceeded three. Typically, one species was found per corpse. Species differed between indoor and outdoor cases, with D. frischii and D. undulatus dominant in outdoor cases, while D. peruvianus dominant in indoor cases. Calliphoridae was found in 88% of the cases, while Hydrotaea and Piophilidae were observed 40% of the time. Regarding Coleoptera, Necrobia spp. (Coleoptera: Cleridae) was observed in 46% of the cases. Lastly, we observed a typical decomposition pattern, with preferential feeding areas on the face, hands, and feet (i.e., the extremities). Pupation chambers on or inside the bones were not observed.

  11. Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico

    Directory of Open Access Journals (Sweden)

    Sonia Maria Noemberg Lazzari

    2006-06-01

    Full Text Available Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico. O objetivo desta pesquisa foi avaliar o efeito do resfriamento artificial de grãos de arroz para o controle de coleópteros-praga. O ar frio foi insuflado pelo sistema de aeração em um silo metálico com arroz-em-casca. A avaliação do tratamento foi feita quinzenalmente usando armadilhas caladores. As espécies de Coleoptera capturadas foram: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16,5% e Sitophilus spp. (0,5%. Aos 28 dias, a temperatura média da massa de grãos era de 15ºC, e o número médio de insetos havia diminuído 76,8%. A aplicação de ar frio manteve as populações sob controle por aproximadamente 60 dias. Os resultados do monitoramento dos insetos e da temperatura indicaram que um novo ciclo de ar frio deveria ser aplicado nesse período para manter as populações sob controle. Também o manejo adequado da massa de grãos faz-se necessário para garantir resultados satisfatórios do resfriamento artificial.Artificial chilling to control Coleoptera in paddy rice stored in metallic silo. The objective of this research was to evaluate the effect of artificial chilling for the control of coleopterans in stored paddy rice. The cold air was insufflated through the aeration system of a metallic silo with paddy rice. Evaluation of insect number was made every 15 days using probe traps. The species of Coleoptera captured were: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16.5% and Sitophilus spp. (0.5%. By the 28th day the average temperature of the grain mass was 15ºC, and the mean number of insects decreased 76.8%. The cold air application kept the insect populations under control for approximately 60 days. The results of temperature and insect monitoring indicated that a new cycle of cold air should be applied by that time to keep the populations under

  12. Inconspicuous structural coloration in the elytra of beetles Chlorophila obscuripennis (Coleoptera)

    Science.gov (United States)

    Liu, Feng; Yin, Haiwei; Dong, Biqin; Qing, Youhua; Zhao, Li; Meyer, Serge; Liu, Xiaohan; Zi, Jian; Chen, Bin

    2008-01-01

    The elytra of male beetles Chlorophila obscuripennis (Coleoptera) display an inconspicuous iridescent bluish green color. By structural characterizations we find that the outermost elytral surface comprises a sculpted multilayer, which is the origin of structural coloration. In elytra both structural green and cyan colors are observed which arise from the modulations imposed on the multilayer, leading to a bluish green color by color mixing. The adoption of the sculpted multilayer can render structural coloration inconspicuous, which could be advantageous for camouflage. In addition, it can cause light emergence at nonspecular angles.

  13. Gross anatomy of central nervous system in firefly, Pteroptyx tener (Coleoptera: Lampyridae)

    Science.gov (United States)

    Hudawiyah, Nur; Wahida, O. Nurul; Norela, S.

    2015-09-01

    This paper describes for the first time the organization and fine structure of the central nervous system (CNS) in the fireflies, Pteroptyx tener (Coleoptera: Lampyridae). The morphology of the CNS was examined by using Carl Zeiss AxioScope A1 photomicroscope with iSolution Lite software. Some specific structural features such as the localization of protocerebrum, deutocerebrum and tritocerebrum in the brain region were analyzed. Other than that, the nerve cord and its peripheral structure were also analyzed. This study suggests that, there is a very obvious difference between male and female central nervous system which illustrates that they may differ in function in controlling physiological and behavioral activities.

  14. Australian marsh beetles (Coleoptera: Scirtidae). 2. Pachycyphon, a new genus of presumably terrestrial Australian Scirtidae.

    Science.gov (United States)

    Zwick, Peter

    2013-01-01

    The genus Pachycyphon is erected for marsh beetles (Coleoptera: Scirtidae) from tropical rainforests in Queensland, Australia. The following species are included: P. corpulentus sp. n., P. crassus sp. n., P. elegans sp. n., P. funicularis sp. n., P. gravis sp. n., P. monteithi sp. n., P. obesus sp. n., P. pinguis sp. n., P. serratus sp. n., P. turgidus sp. n. (the type species). Females are wingless and have fossorial ovipositors, a terrestrial larval life is therefore hypothesized. Pachycyphon is compared with other genera, especially probable relatives from temperate rainforests in southeastern Australia.

  15. Invertebrate fauna (Coleoptera, Collembola, Diplopoda, Isopoda collected in the karst areas of the Aninei - Locvei Mountains

    Directory of Open Access Journals (Sweden)

    Andrei Giurginca

    2011-08-01

    Full Text Available The authors identified 132 species of invertebrates (14 Oniscidea, 25 Diplopoda,31 Collembola and 62 Coleoptera recently sampled (2001–2006 from the soil and subterranean (MSS and caves environments from the Banat Mountains. Some new,rare and endemic species are discussed. The seasonal changes of the species diversity in the superficial subterranean environments at 0.5 to 1 m in depth are for the first time presented for the Reşiţa – Moldova Nouă synclinorium. The characteristic and preferential species for the mesovoid shallow substratum (MSS, belonging to the analyzed taxa, are identified.

  16. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-09-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  17. Development of an attractant-baited trap for Oxythyrea funesta Poda (Coleoptera: Scarabaeidae, Cetoniinae).

    Science.gov (United States)

    Vuts, József; Imrei, Zoltán; Töth, Miklós

    2008-01-01

    In electroantennographic tests isosafrol, methyl salicylate, (+/-)-lavandulol, geraniol, (E)-anethol, and beta-ionone evoked the largest responses from antennae of female or male Oxythyrea funesta (Coleoptera: Scarabaeidae, Cetoniinae) adult beetles. In field trapping tests in Hungary the 1:1 blend of (+/-)-lavandulol and 2-phenylethanol attracted significantly more adult O. funesta than the single compounds. The addition of (E)-anethol, a previously described attractant for the species, was without effect. There was no difference in the responses of male or female beetles. The binary 2-phenylethanol/(+/-)-lavandulol bait described, in this study is recommended for the use in traps of O. funesta for agricultural purposes.

  18. A review of the natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries.

    Science.gov (United States)

    Di Iorio, Osvaldo

    2014-01-01

    A compilation of the known natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries is provided. Food items of adult Cetoniinae include pollen and/or nectar (flower visitors), sap and/or slime flux, ripened fruits on plants, green tissues and leaves, and honey. Of the 36 species of Cetoniinae from Argentina, food items are known only for 11 species (30.5%). Attraction to light and bait-traps, adult activity periods, vertebrate predators, and the occurrence in bird nests are presented and discussed. Other insects that share the same food sources and bait-traps with Cetoniinae are mentioned. PMID:24869870

  19. Espécies de Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini ocorrentes na Bolívia

    Directory of Open Access Journals (Sweden)

    Maria Helena M. Galileo

    2013-03-01

    Full Text Available Species of Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini occurring in Bolivia. The genus Gorybia (Cerambycinae, Piezocerini consists of 45 described species with seven species recorded from Bolivia. Nine new species are described herein from Bolivia: G. abnormalis sp. nov.; G. alveolata sp. nov.; G. asyka sp. nov.; G. florida sp. nov.; G. inarmata sp. nov.; G. longithorax sp. nov.; G. guenda sp. nov.; G. tuberosa sp. nov. and G. wappesi sp. nov. A key to the species now known to occur in Bolivia is included.

  20. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-06-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  1. PITYOPHAGUS QUERCUS REITTER, 1877, A NEW SAPROXYLIC SAP BEETLE FOR THE ITALIAN FAUNA (Coleoptera, Nitidulidae

    Directory of Open Access Journals (Sweden)

    Paolo Audisio

    2011-10-01

    Full Text Available During ecological investigations on saproxylic beetle communities of central Italy (Latium, at Bosco Polverino (a mixed evergreen/deciduous forest fragment dominated by cork oaks, and at Allumiere (a small fragment of beech forest surrounded by turkey oak stands, the authors found three specimens of Pityophagus quercus Reitter, 1877 (Coleoptera, Nitidulidae. These are the first known records of this species in Italy, and the first one in association with an evergreen oak, Quercus suber. This discovery led us to review both bionomical and faunistic data so far available on this exceedingly rare and poorly known species.

  2. Redescription of Platynaspis flavoguttata (Gorham (Coleoptera, Coccinellidae and notes on nomenclature of Platynaspis kapuri Chakraborty & Biswas

    Directory of Open Access Journals (Sweden)

    J. Poorani

    2014-05-01

    Full Text Available Platynaspis flavoguttata (Gorham (Coleoptera: Coccinellidae is redescribed and the male genitalia are illustrated for the first time. It is also recorded from Sri Lanka for the first time. Platynaspis bimaculata (Hoang, 1983 is a new junior synonym of Platynaspis bimaculata Pang & Mao, 1979 (new synonym. Platynaspis kapuri Chakraborty & Biswas, 2000, the replacement name for Platynaspis bimaculata Pang & Mao, 1979 established by Ukrainsky (2007, is also the new replacement name for Platynaspis bimaculata (Hoang, 1983, as both are junior homonyms of Platynaspis bimaculata Weise, 1888 besides being synonyms. Platynaspis hoangi Ukrainsky (2007 is an unnecessary replacement name for P. bimaculata (Hoang.

  3. CONTRIBUTIONS TO THE KNOWLEDGE OF THE SPECIES OF BEETLES (INSECTA: COLEOPTERA FROM WHEAT CROP

    Directory of Open Access Journals (Sweden)

    Mihai Tălmaciu

    2016-07-01

    Full Text Available This paper presents the species of beetles (Insecta: Coleoptera between in wheat crops from Tişiţa, in Vrancea county. The observations were made in a crop of wheat in 2013, who were placed the soil traps type Barber, during the two months, May and June. It was used three variants: • Variant 1 - consumption wheat untreated • Variant 2 - consumption wheat treaty • Variant 3 - treated wheat seed The gathering of samples from the traps was done periodically, every 12-15 days. The most species frequent gathered was: Pentodon idiota, Epicometis hirta, Opatrum sabulosum, Phyllotreta atra, Phyllotreta nemorum, Tanymecus dilaticollis.

  4. Efficacies of spinosad and a combination of chlorpyrifos-methyl and deltamethrin against phosphine-resistant Rhyzopertha dominica (Coleoptera: Bostrichidae) and Tribolium castaneum (Coleoptera: Tenebrionidae) on wheat.

    Science.gov (United States)

    Bajracharya, N S; Opit, George P; Talley, J; Jones, C L

    2013-10-01

    Highly phosphine-resistant populations of Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) have recently been found in Oklahoma grain storage facilities. These findings necessitate development of a phosphine resistance management strategy to ensure continued effective use of phosphine. Therefore, we investigated the efficacies of two grain insecticides, namely, spinosad applied at label rate of 1 ppm and a mixture of chlorpyrifos-methyl and deltamethrin applied at label rates of 3 and 0.5 ppm, respectively, against highly phosphine-resistant R. dominica and T. castaneum. Adult mortality and progeny production suppression of spinosad- or chlorpyrifos-methyl + deltamethrin mixture-treated wheat that had been stored for 2, 84, 168, 252, and 336 d posttreatment were assessed. We found that both spinosad and chlorpyrifos-methyl + deltamethrin were effective against phosphine-resistant R. dominica and caused 83-100% mortality and also caused total progeny production suppression for all storage periods. Spinosad was not effective against phosphine-resistant T. castaneum; the highest mortality observed was only 3% for all the storage periods. Chlorpyrifos-methyl + deltamethrin was effective against phosphine-resistant T. castaneum only in treated wheat stored for 2 and 84 d, where it caused 93-99% mortality. However, chlorpyrifos-methyl + deltamethrin was effective and achieved total suppression of progeny production in T. castaneum for all the storage periods. Spinosad was not as effective as chlorpyrifos-methyl + deltamethrin mixture at suppressing progeny production of phosphine-resistant T. castaneum. These two insecticides can be used in a phosphine resistance management strategy for R. dominica and T. castaneum in the United States.

  5. Estirpes de Bacillus thuringiensis efetivas contra insetos das ordens Lepidoptera, Coleoptera e Diptera Bacillus thuringiensis strains effective against insects of Lepidoptera, Coleoptera and Diptera orders

    Directory of Open Access Journals (Sweden)

    Lílian Botelho Praça

    2004-01-01

    Full Text Available O objetivo deste trabalho foi selecionar entre 300 estirpes de Bacillus thuringiensis as efetivas simultaneamente contra larvas de Spodoptera frugiperda J.E. Smith e Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae, Anthonomus grandis Boheman (Coleoptera: Curculionidae, Aedes aegypti Linnaeus e Culex quinquefasciatus Say (Diptera: Culicidae. Foram selecionadas duas estirpes de B. thuringiensis, denominadas S234 e S997, que apresentaram atividade contra as três ordens de insetos. As estirpes foram caracterizadas por métodos morfológicos, bioquímicos e moleculares. As mesmas apresentaram duas proteínas principais de 130 e 65 kDa, produtos de reação em cadeia da polimerase de tamanho esperado para a detecção dos genes cry1Aa, cry1Ab, cry1Ac, cry1B e cry2 e cristais bipiramidais, cubóides e esféricos.The aim of this work was to select among 300 strains of Bacillus thuringiensis those which are simultaneously effective against larvae of Spodoptera frugiperda J.E. Smith and Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae, Anthonomus grandis Boheman (Coleoptera: Curculionidae, Aedes aegypti Linnaeus and Culex quinquefasciatus Say (Diptera: Culicidae. Two strains of B. thuringiensis were selected, S234 and S997, which presented activity against those three insect orders. Both strains were characterized by morphological, biochemical and molecular methods. They have presented two main proteins with 130 and 65 kDa, polimerase chain reaction products with expected sizes for detection of the genes cry1Aa, cry1Ab, cry1Ac, cry1B and cry2 and bipiramidal, cubical and spherical crystals.

  6. Phylogenetically informative rearrangements in mitochondrial genomes of Coleoptera, and monophyly of aquatic elateriform beetles (Dryopoidea).

    Science.gov (United States)

    Timmermans, Martijn J T N; Vogler, Alfried P

    2012-05-01

    Mitochondrial gene order in Coleoptera has been thought to be conservative but a survey of 60 complete or nearly complete genomes revealed a total of seven different gene rearrangements (deletions, gene order reversals), mainly affecting tRNA genes. All of these were found to be limited to a single taxon or a subclade of Coleoptera. The phylogenetic distribution of a translocation of tRNA(Pro) in three species of elateriform beetles was investigated further by sequencing three nearly complete mitochondrial genomes (Dascillidae, Byrrhidae, Limnichidae) and ten additional individuals for a ∼1370 bp diagnostic fragment spanning the relevant region. Phylogenetic analysis consistently recovered the monophyly of families previously grouped in the contentious superfamily Dryopoidea, a group of approximately 10 beetle families with mainly aquatic lifestyles. The Byrrhidae (moss beetles) were not part of this lineage, although they may be its sister group, to recover the widely accepted Byrrhoidea. The tRNA(Pro) translocation was present in all members of Dryopoidea, but not in any other Elateriformia, providing independent support for this lineage and for a single origin of aquatic habits.

  7. The hydraulic mechanism in the hind wing veins of Cybister japonicus Sharp (order: Coleoptera).

    Science.gov (United States)

    Sun, Jiyu; Wu, Wei; Ling, Mingze; Bhushan, Bharat; Tong, Jin

    2016-01-01

    The diving beetles (Dytiscidae, Coleoptera) are families of water beetles. When they see light, they fly to the light source directly from the water. Their hind wings are thin and fragile under the protection of their elytra (forewings). When the beetle is at rest the hind wings are folded over the abdomen of the beetle and when in flight they unfold to provide the necessary aerodynamic forces. In this paper, the unfolding process of the hind wing of Cybister japonicus Sharp (order: Coleoptera) was investigated. The motion characteristics of the blood in the veins of the structure system show that the veins have microfluidic control over the hydraulic mechanism of the unfolding process. A model is established, and the hind wing extending process is simulated. The blood flow and pressure changes are discussed. The driving mechanism for hydraulic control of the folding and unfolding actions of beetle hind wings is put forward. This can assist the design of new deployable micro air vehicles and bioinspired deployable systems. PMID:27547607

  8. Ophiostoma species (Ascomycetes: Ophiostomatales) associated with bark beetles (Coleoptera: Scolytinae) colonizing Pinus radiata in northern Spain.

    Science.gov (United States)

    Romón, Pedro; Zhou, XuDong; Iturrondobeitia, Juan Carlos; Wingfield, Michael J; Goldarazena, Arturo

    2007-06-01

    Bark beetles (Coleoptera: Scolytinae) are known to be associated with fungi, especially species of Ophiostoma sensu lato and Ceratocystis. However, very little is known about these fungi in Spain. In this study, we examined the fungi associated with 13 bark beetle species and one weevil (Coleoptera: Entiminae) infesting Pinus radiata in the Basque Country of northern Spain. This study included an examination of 1323 bark beetles or their galleries in P. radiata. Isolations yielded a total of 920 cultures, which included 16 species of Ophiostoma sensu lato or their asexual states. These 16 species included 69 associations between fungi and bark beetles and weevils that have not previously been recorded. The most commonly encountered fungal associates of the bark beetles were Ophiostoma ips, Leptographium guttulatum, Ophiostoma stenoceras, and Ophiostoma piceae. In most cases, the niche of colonization had a significant effect on the abundance and composition of colonizing fungi. This confirms that resource overlap between species is reduced by partial spatial segregation. Interaction between niche and time seldom had a significant effect, which suggests that spatial colonization patterns are rarely flexible throughout timber degradation. The differences in common associates among the bark beetle species could be linked to the different niches that these beetles occupy. PMID:17668036

  9. DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR Arzu ÜNAL, Ahmet ÇABUK, Nazif KOLONKAYA

    Directory of Open Access Journals (Sweden)

    Nazif KOLANKAYA

    2011-08-01

    Full Text Available Detoxification of a chlorinated phenolic compound, 2,4,6- trichlorophenol through treatment with laccase enzyme produced by a white rot fungus, Trametes versicolor was investigated. Enzymaticdechlorination experiments by using free and immobilized laccase have been performed in a lab scale bioreactor. Chlorine ion and dissolved oxygen electrodes mounted to the bioreactor were used continuouslyto detect the profiles of chlorine ions and oxygen consumption, respectively, in reaction medium. The maximum dechlorination activity of laccase for free and immobilized form was determined as 160 μM of substrate concentration at pH 5.0, 25 °C, and 30 min of incubation time. Also, GC/MS analyses of enzymatic degradation products indicated that chlorine removal was a result of degradation of 2,4,6- trichlorophenol by the laccase under the determined optimum conditions.

  10. ESCARABAJOS TIGRE (COLEOPTERA: CICINDELIDAE DEL MUSEO ENTOMOLÓGICO FRANCISCO LUÍS GALLEGO: NUEVOS REGISTROS PARA DEPARTAMENTOS DE COLOMBIA TIGER BEETLES (COLEOPTERA: CICINDELIDAE AT THE ENTOMOLOGICAL MUSEUM FRANCISCO LUÍS GALLEGO: NEW RECORDS

    Directory of Open Access Journals (Sweden)

    Manuel Alejandro Ramírez Mora

    2008-06-01

    Full Text Available Se registran por primera vez las especies de escarabajos tigre (Coleoptera: Cicindelidae presentes en el Museo Entomológico Francisco Luis Gallego (MEFLG. Se identificaron 167 especimenes distribuidos en ocho géneros y 27 especies, se reportan 24 nuevos registros para diferentes departamentos de Colombia. Se señalan aspectos importantes de la taxonomía y sistemática del grupo. Además, se presentan comentarios biológicos y de distribución de las especies.Tiger beetles (Coleoptera: Cicindelidae species at the Museo Entomológico Francisco Luis Gallego (MEFLG are registered by first time. 167 specimens in 8 genus and 27 species were identified, 24 new records for different Colombian states are reported. Some important aspects of the group’s taxonomy and systematic are pointed. Additionally, species’ biological and distributional comments are presented.

  11. 483例儿童花斑癣回顾性分析%Pityriasis versicolor in children: a retrospective study of 483 cases

    Institute of Scientific and Technical Information of China (English)

    施和建; 张国龙; 杜旭峰; 周晴; 王涛; 邵敏华; 黄海峰

    2008-01-01

    目的总结及分析儿童花斑癣患者的临床特点,比较与成人花斑癣的异同点.方法就诊于我院皮肤科门诊的花斑癣患者认真填写问卷调查表,内容包括:性别、年龄、发病年龄,病程、皮损部位、诱因、发病季节、家族史.并进行真菌学检查.结果儿童花斑癣患者共483例,其中男286例,占59.2%,女197例,占40.8%.年龄15天至12岁.所有患儿面部发病207例,占42.9%,其中发生于前额的共143例,占面部发病的69.1%.多汗患儿405例,占83.9%.有家族史38例,占7.9%.所有患儿均采用1%联苯苄唑乳膏外用,治疗2周以上,447例患儿通过治疗后得到有效的临床症状改善及病原学疗效,总有效率为92.5%.结论儿童花斑癣同成人花斑癣比较有不同的临床特点,发病因素与成人不尽相同,且在诊断上需与儿童面部色素减退性皮肤病进行鉴别.%Objective To assess the clinical characteristics of pityriasis versicolor (PV) in children vs adults. Methods Questionnaire was designed and given to consecutive patients with PV in the Department of Dermatology, Wuxi Children's Hospital, for collection of information on their sex, age, onset age, duration of disease, localization of lesions, inducement, onset season, and family history. Findings on mycological examination before and after medication were assessed. Results Totally, 483 children with PV were enrolled, including 286 (59.2%) boys and 197 (40.8%) girls. Patients aged from 15 days to 12 years. Facial lesions were the most frequent (n = 207, 42.9% ) manifestation with a preference on the forehead (n = 143, 69.1% ). Hyperhidrosis was found in 405 (83.9%) patients, and family history of PV in 38 (7.9%) patients. Patients were instructed to apply bifonazole 1% cream on lesions. After more than 2 weeks of treatment, 447 (92.5%) patients obtained both clinical and mycological improvement. Conclusions Difference is present between pediatric and adult PV in clinical manifestations

  12. Especies de Megacerus (Coleoptera: Bruchidae y sus plantas hospederas en Yucatán Megacerus species (Coleoptera: Bruchidae and their host plants in Yucatán

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    Enrique Reyes

    2009-12-01

    Full Text Available Se registra la incidencia de Megacerus (Coleoptera: Bruchidae en semillas de 7 especies de Convolvulaceae de 6 sitios al norte de Yucatán. Las semillas de Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia y M. cissoides recolectadas en etapa de dispersión fueron colocadas en sobres limpios de papel y puestas en condiciones de laboratorio hasta la emergencia de los brúquidos. Se obtuvieron 1111 coleópteros pertenecientes a 5 especies: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, y M. (Serratibruchus cubiciformis. Las semillas de I. crinicalyx, M. aegyptia y M. cissoides fueron infestadas principalmente por M. (M. porosus, las de I. hederifolia e I. triloba por M. (M. cubiculus y las de I. nil por M. (M. tricolor. Todos los individuos de Megacerus (M. sp., fueron encontrados en semillas de J. penthanta. En el nivel génerico de hospedero se encontró que las semillas de Ipomoea fueron infestadas principalmente por M. (M. cubiculus y las de Merremia por M. (M. porosus. Estos resultados amplían el rango de plantas hospederas registrado para las especies de Megacerus y resaltan la estrecha asociación que existe entre un linaje de depredadores de semillas Bruchidae y plantas de la familia Convolvulaceae.Seed-attacking by Megacerus (Coleoptera: Bruchidae is reported for 7 Convolvulaceae species from 6 sites at the North of Yucatan. Seeds of Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia and M. cissoides were collected and placed in clean envelopes under laboratory conditions to collect all bruchids emerged from seeds. A total of 1111 coleopterans were hatched from seeds, and 5 taxa were identify: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, and M. (Serratibruchus cubiciformis. Seeds of I. crinicalyx, M. aegyptia and M. cissoides were attacked mainly by M. (M. porosus; I

  13. Longhorned Beetles Collection of the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey (Coleoptera,Cerambycidae)

    OpenAIRE

    ÖZDİKMEN, Hüseyin; ŞAHİN, Özlem

    2010-01-01

    In the present paper, specimens of Cerambycidae were examined in the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey. As a result of identification of these specimens, thirty-eight species and two subspecies belonging to twenty-eight genera of five subfamilies were determined. With this paper, new faunistic data and some zoogeographical evaluations were presented on longhorned beetles fauna (Coleoptera, Cerambycidae) of Turkey. The faunistic data in the pr...

  14. A new genus and species of Schizogyniidae (Acari: Mesostigmata) associated with carabid beetles (Coleoptera: Carabidae) from Ukraine.

    Science.gov (United States)

    Trach, Viacheslav A; Seeman, Owen D

    2014-04-29

    A new genus and species of Schizogyniidae (Acari: Mesostigmata: Celaenopsoidea), Euroschizogynium calvum gen. nov. and sp. nov., associated with Scarites terricola Bonelli, 1813 (Coleoptera: Carabidae) is described from Ukraine, representing the first record of the family from the Palaearctic. Fusura civica Valle & Fox, 1966 is moved out of the Schizogyniidae and placed into the Megacelaenopsidae. A new diagnosis for the family Schizogyniidae and a key to genera are provided.

  15. Uji Efektifitas Beberapa Insektisida Nabati Terhadap Mortalitas Hama Sitophilus zeamais Motsch. (Coleoptera; Curculionidae) Pada Benih Jagung (Zea mays)

    OpenAIRE

    Subhan, Fadillah

    2011-01-01

    Fadillah Subhan, "Test Effectiveness of Some Insecticides Against Plant Pests Sitophilus zeamais Motsch Mortality. (Coleoptera: Curculionidae) In the Seed of Corn (Zea mays). "It was under supervised by Yuswani Pangestiningsih and Fatimah Zahara. S. zeamais is a pest of corn in storage that causes substantial yield losses, one of which effective control is the use of botanical insecticides. Testing the effectiveness of some botanical insecticides on mortality of S. zeamais on maize seed plant...

  16. A review of Elocomosta Hansen with a description of a new species with reduced eyes from China (Coleoptera, Hydrophilidae, Sphaeridiinae)

    Science.gov (United States)

    Lin, Renchao; Jia, Fenglong; Fikáček, Martin

    2016-01-01

    Abstract A new species of the genus Elocomosta Hansen, 1989 (Coleoptera: Hydrophilidae: Sphaeridiinae: Coelostomatini), Elocomosta lilizheni sp. n., is described from Guangxi Province, China. It is compared in detail with the only other known species of the genus, Elocomosta nigra Hansen, 1989 from Borneo, and the genus is diagnosed from the remaining coelostomatine genera. The new species is unusual among Hydrophilidae by having extremely reduced eyes. PMID:27551232

  17. Synergistic effects of chlorpyrifos with piperonyl butoxide (pbo) against the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

    OpenAIRE

    Akter Mst Yeasmin; Talukdar Muhammad Waliullah; ASM Shafiqur Rahman

    2014-01-01

    Objective: To investigate the co-toxicity and co-efficient activity of Chlorpyrifos (Dursban 20EC), an organophosphate and Piperonyl butoxide (PBO) against the lesser meal worm Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) (A. diaperinus). Methods: The repellent activity was carried out by the residual film assay technique. Statistically the dose mortality relationship was expressed as a median lethal dose (LD50) by the probit analysis. The regression lines and isoboles were ...

  18. The lectotype of Goliathus drurii Westwood, 1837 (Coleoptera: Scarabaeidae: Cetoniinae) in the Macleay Museum, University of Sydney, Australia.

    Science.gov (United States)

    Blackburn, Robert

    2016-01-01

    The lectotype of Goliathus drurii Westwood (Coleoptera: Scarabaeidae: Cetoniinae) has been located to the Macleay Museum, University of Sydney, Australia. Collected in 1775, this specimen represents the second species of Goliathus to be illustrated in European literature, however eluding a proper species description until 1837. This important specimen, overlooked by previous curators, represents another portion of the Dru Drury collections purchased by Alexander McLeay now held in the Macleay Museum. PMID:27615919

  19. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

    OpenAIRE

    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineens...

  20. Natural history of Belonuchus Nordmann spp. and allies (Coleoptera: Staphylinidae) in Heliconia L. (Zingiberales: Heliconiaceae) flower bracts

    OpenAIRE

    Frank, J. Howard; Barrera, Roberto

    2010-01-01

    Adults, and in some species larvae, of several members of Belonuchus Nordmann (Coleoptera: Staphylinidae: Staphylininae) and a few related genera seem to be to various degrees consistently associated with flower bracts of the genus Heliconia (Zingiberales: Heliconiaceae). They are predators and eat various dipterous and lepidopterous larvae in that habitat. Adults of at least Belonuchus cephalotes (Sharp) and Odontolinus fasciatus Sharp are able to immerse completely in water to capture larva...

  1. The mortality of Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae induced by powdered plants

    Directory of Open Access Journals (Sweden)

    Kłyś Małgorzata

    2015-01-01

    Full Text Available The aim of this study was to investigate whether powdered plants of different species namely: peppermint Mentha piperita (L. (Lamiaceae, wormwood Artemisia absinthium (L. (Asteraceae, common sage Salvia officinalis (L. (Lamiaceae, allspice Pimenta dioica (Linnaeus et Merrill (Myrtaceae and common garlic Allium sativum (L. (Amaryllidaceae, added to semolina using concentrations of 1.23, 3.61, and 5.88%, influence the mortality rate in the saw-toothed grain beetle Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae. Experiments were conducted in a laboratory at 28°C and relative humidity 60±5%. At the concentration of 1.23%, allspice seeds caused the highest mortality amongst the saw-toothed grain beetle. When concentrations of 3.61 and 5.88% were used, sage, peppermint and wormwood caused the highest statistically significant mortality of O. surinamensis

  2. Carpophilus zeaphilus, a new sap beetle species acclimatized in Italy (Coleoptera: Nitidulidae

    Directory of Open Access Journals (Sweden)

    Paolo Audisio

    2015-06-01

    Full Text Available Carpophilus zeaphilus Dobson, 1969 (Coleoptera, Nitidulidae, Carpophilinae is an Afrotropical species that has become widespread in Sub-Saharan Africa, the Arabian Peninsula, and southern Mediterranean areas in recent years. The species was first recorded from Europe in Portugal and Spain nearly thirty years ago, and it was later intercepted in Sicily near Trapani in 1991. A few specimens of this species were collected in April, 2015 in a sparsely forested area near Rome, which suggests a recent acclimatization into peninsular Italy. Specimens were taken on flowering trees of Prunus spinosa L. (Rosaceae, an unusual occurrence for most introduced species of Carpophilinae that are normally associated with rotten fruit and other decomposing vegetal matter.

  3. Beetles (Insecta, Coleoptera) associated with pig carcasses exposed in a Caatinga area, Northeastern Brazil.

    Science.gov (United States)

    Santos, W E; Alves, A C F; Creão-Duarte, A J

    2014-08-01

    The species richness, abundance and seasonality of Coleoptera fauna associated with pig carcasses exposed in a Caatinga area were examined. Tray, pitfall and modified Shannon traps were settled together to collect these insects during two seasons (dry and rainy). 4,851 beetles were collected, belonging to 19 families and 88 species. Staphylinidae (2,184) and Histeridae (1,264) were the most abundant families and accounted for 71.1% of the specimens collected. Scarabaeidae (15) showed the highest species richness. The most abundant species were Atheta iheringi Bernhauer, 1908 (Staphylinidae) (1,685), Euspilotus sp. (Histeridae) (461), Stelidota geminata (Say, 1825) (Nitidulidae) (394), Xerosaprinus diptychus (Marseul, 1855) (Histeridae) (331) and Dermestes maculatus De Geer, 1774 (Dermestidae). Amongst these species, X. diptychus showed to be strongly influenced by seasonality, since 96.1% of the specimens were collected during the dry season.

  4. The tiger beetles (Coleoptera: Carabidae, Cicindelinae) of Angola: a descriptive catalogue and designation of neotypes.

    Science.gov (United States)

    Serrano, Artur R M; Capela, Rúben A

    2013-11-01

    An annotated catalogue of the species and subspecies of tiger beetles (Coleoptera: Carabidae, Cicindelinae) hitherto known from Angola is given. A total of 89 forms (74 species and 15 subspecies) is recorded from this southwestern country of Africa. Within this assemblage there are 31 endemic forms (33.3%). Some species are represented by only the holotype specimen (some without locality) or the type series. Others were recorded based on a single specimen. Records for six species previously unknow from Angola are given: Foveodromica sp. n. 1, Foveodromica sp. n. 2, Ophryodera rufomarginata bradshawi Péringuey, 1888, Elliptica muata parallelestriata (W. Horn, 1923), Lophyra differens (W. Horn, 1892) and Myriochila jucunda (Péringuey, 1892). A historical review, as well as some considerations on the distribution and conservation status of these beetles in Angola are also presented.

  5. Effects of pitfall trap preservative on collections of carabid beetles (Coleoptera: Carabidae)

    Science.gov (United States)

    McCravy, K.W.; Willand, J.E.

    2007-01-01

    Effects of six pitfall trap preservatives (5% acetic acid solution, distilled water, 70% ethanol, 50% ethylene glycol solution, 50% propylene glycol solution, and 10% saline solution) on collections of carabid beetles (Coleoptera: Carabidae) were studied in a west-central Illinois deciduous forest from May to October 2005. A total of 819 carabids, representing 33 species and 19 genera, were collected. Saline produced significantly fewer captures than did acetic acid, ethanol, ethylene glycol, and propylene glycol, while distilled water produced significantly fewer captures than did acetic acid. Significant associations between numbers of captures and treatment were seen in four species: Amphasia interstitialis (Say), Calathus opaculus LeConte, Chlaenius nemoralis Say, and Cyclotrachelus sodalis (LeConte). Results of this study suggest that type of preservative used can have substantial effects on abundance and species composition of carabids collected in pitfall traps.

  6. Discovery of mycangia and the associated xylose-fermenting yeasts in stag beetles (Coleoptera: Lucanidae)

    Science.gov (United States)

    Tanahashi, Masahiko; Kubota, Kôhei; Matsushita, Norihisa; Togashi, Katsumi

    2010-03-01

    Most wood-feeding insects need an association with microbes to utilize wood as food, and some have special organs to store and convey the microbes. We report here the discovery of the microbe-storage organ (mycangium) in stag beetles (Coleoptera: Lucanidae), which develop in decayed wood. The mycangium, which was discovered in the abdomen, is present in all adult females of 22 lucanid species examined in this study, but absent in adult males. By contrast, adult insects of both sexes of selected Passalidae, Geotrupidae, and Scarabaeidae, which are related to Lucanidae, lacked mycangia similar to those of the lucanid species. Yeast-like microbes were isolated from the mycangium of five lucanid species. DNA sequence analyses indicate that the microbes are closely related to the xylose-fermenting yeasts Pichia stipitis, Pichia segobiensis, or Pichia sp. known from the gut of a passalid species.

  7. Synthesis, antifeedant activity against Coleoptera and 3D QSAR study of alpha-asarone derivatives.

    Science.gov (United States)

    Łozowicka, B; Kaczyński, P; Magdziarz, T; Dubis, A T

    2014-01-01

    For the first time, a set of 56 compounds representing structural derivatives of naturally occurring alpha-asarone as an antifeedants against stored product pests Sitophilus granarius L., Trogoderma granarium Ev., and Tribolium confusum Duv., were subjected to the 3D QSAR studies. Three-dimensional quantitative structure-activity relationships (3D-QSAR) for 56 compounds, including 15 newly synthesized, were performed using comparative molecular field analysis s-CoMFA and SOM-CoMSA techniques. QSAR was conducted based on a combination of biological activity (against Coleoptera larvae and beetles) and various geometrical, topological, quantum-mechanical, electronic, and chromatographic descriptors. The CoMSA formalism coupled with IVE (CoMSA-IVE) allowed us to obtain highly predictive models for Trogoderma granarium Ev. larvae. We have found that this novel method indicates a clear molecular basis for activity and lipophilicity. This investigation will facilitate optimization of the design of new potential antifeedants. PMID:24601760

  8. Ground beetle (Coleoptera: Carabidae) assemblages in narrow hedgerows in a Danish agricultural landscape

    DEFF Research Database (Denmark)

    Lövei, G. L.; Magura, T.

    2015-01-01

    beetle assemblages. The number of ground beetle individuals and species were significantly the highest in the hawthorn hedges and significantly decreased from the hedges with rowan toward the spruce hedges. The elevated number of ground beetle individuals and species in the hawthorn hedges were due......The role of hedgerows in supporting ground beetles (Coleoptera: Carabidae) in a Danish agricultural landscape was examined. Nine old, well established single-row hedges were selected for the study, three each of a native species (hawthorn, Crataegus monogyna), a non-native deciduous one (rowan...... to the forest specialist species, as the number of forest specialist ground beetle individuals and species were significantly higher in the hawthorn hedges compared to the hedges with rowan and spruce. Differences in the number of the grassland and the cropland specialist ground beetle individuals and species...

  9. The scarab beetle tribe Pentodontini (Coleoptera: Scarabaeidae: Dynastinae) of Colombia: taxonomy, natural history, and distribution.

    Science.gov (United States)

    López-García, Margarita M; Gasca-Álvarez, Héctor J; Amat-García, Germán

    2015-11-27

    Pentodontini is the most diverse tribe of Dynastinae (Coleoptera: Scarabaeidae), and most of the genera are restricted to a single biogeographic region. In this work, the taxonomic composition of the Pentodontini in Colombia was determined, and genera and species were diagnosed based on external morphology and male genitalia. Records of 1,580 specimens from 31 departments and 398 localities in Colombia were obtained from 24 species in the genera Bothynus Hope, Denhezia Dechambre, Euetheola Bates, Hylobothynus Ohaus, Oxyligyrus Arrow, Parapucaya Prell, Pucaya Ohaus, and Tomarus Erichson. Oxyligyrus cayennensis Endrödi, Tomarus cicatricosus (Prell), and T. pullus (Prell) are reported for the first time from Colombia. Pucaya punctata Endrödi is reduced to synonymy with Pucaya pulchra Arrow. Possible changes in the classification of Denhezia Dechambre are discussed. Dichotomous keys are provided for Colombian genera and species. Taxonomic descriptions and distribution maps are included for all species.

  10. Cellulose digestion in Monochamus marmorator Kby. (coleoptera: Cerambycidae): role of acquired fungal enzymes

    Energy Technology Data Exchange (ETDEWEB)

    Kukol, J.J.; Martin, M.M.

    1986-05-01

    Larvae of the balsam fir sawyer, Monochamus marmorator Kby. (Coleoptera, Cerambycidae), contain midgut digestive enzymes active against hemicellulose and cellulose. Cellulases from larvae fed on balsam fir wood infected with the fungus, Trichoderma harzianum Rifai (Deuteromycetes, Moniliales, Moniliaceae), were found to be identical to those of the cellulase complex produced by this fungus when compared using chromatography, electrophoresis, and isofocusing. When larvae are maintained on a fungusfree diet, their midgut fluids lack cellulolytic activity, and they are unable to digest cellulose. Cellulolytic capacity can be restored by feeding the larvae wood permeated by fungi. We conclude that the enzymes which enable M. marmorator larvae to digest cellulose are not produced by the larvae. Instead, the larvae acquire the capacity to digest cellulose by ingesting active fungal cellulases while feeding in fungus-infected wood.

  11. A importância forense de Oxelytrum discicolle (Brullé, 1840) (Coleoptera, Silphidae)

    OpenAIRE

    Camila Kraiczyi Kotzko; V. Costa-Silva; E. G. Silva

    2015-01-01

    Oxelytrum discicolle Brullé, 1840 é a espécie mais coletada de Silphidae (Coleoptera) na América Latina, constituindo um indicador forense na região Neotropical. O objetivo do trabalho é reunir informações sobre biologia e distribuição de O. discicolle, além de ferramentas para utilização desse besouro como indicador forense em casos de morte de humanos. O. discicolle é um besouro de grande porte, de cor preta com um colar vermelho. Larvas da espécie são necrófagas, já os adultos são onívoros...

  12. NEW CONTRIBUTION CONCERNING THE MASS REARING OF TANYMECUS DILATICOLLIS GYLL (COLEOPTERA: CURCULIONIDAE UNDER CONTROLLED CONDITIONS

    Directory of Open Access Journals (Sweden)

    Traian MANOLE

    2013-12-01

    Full Text Available Controlled growth in the laboratory of some species of insect’s are justified both scientifically and practically. Due to the economic importance that the corn crop pest Tanymecus dilaticollis Gyll. (Coleoptera: Curculionidae had in Romania like the main pest of maize crops we tried to obtain successive generations under environment controlled of the insect mentioned. In this paper we aim to improve the efficiency of the multiplication of the species with respect to two aspects: attending larval and multiplication high efficiency. The coefficient value of 20, 90 obtained by us indicate a considerable increase in performance compared to previous attempts multiplication thus increasing your chances of recovery of this method of mass multiplication.

  13. What do we know about winter active ground beetles (Coleoptera, Carabidae in Central and Northern Europe?

    Directory of Open Access Journals (Sweden)

    Radomir Jaskula

    2011-05-01

    Full Text Available This paper summarizes the current knowledge on winter active Carabidae in Central and Northern Europe. In total 73 winter active species are listed, based on literature and own observations. Ground beetles are among the three most numerous Coleoptera families active during the autumn to spring period. The winter community of Carabidae is composed both of larvae (mainly autumn breeding species and adults, as well as of epigeic species and those inhabiting tree trunks. Supranivean fauna is characterized by lower species diversity than the subnivean fauna. The activity of ground beetles decreases in late autumn, is lowest during mid-winter and increases in early spring. Carabidae are noted as an important food source in the diet of insectivorous mammals. They are also predators, hunting small winter active invertebrates.

  14. Incorporating a Sorghum Habitat for Enhancing Lady Beetles (Coleoptera: Coccinellidae in Cotton

    Directory of Open Access Journals (Sweden)

    P. G. Tillman

    2012-01-01

    Full Text Available Lady beetles (Coleoptera: Coccinellidae prey on insect pests in cotton. The objective of this 2 yr on-farm study was to document the impact of a grain sorghum trap crop on the density of Coccinellidae on nearby cotton. Scymnus spp., Coccinella septempunctata (L., Hippodamia convergens Guérin-Méneville, Harmonia axyridis (Pallas, Coleomegilla maculata (De Geer, Cycloneda munda (Say, and Olla v-nigrum (Mulsant were found in sorghum over both years. Lady beetle compositions in sorghum and cotton and in yellow pyramidal traps were similar. For both years, density of lady beetles generally was higher on cotton with sorghum than on control cotton. Our results indicate that sorghum was a source of lady beetles in cotton, and thus incorporation of a sorghum habitat in farmscapes with cotton has great potential to enhance biocontrol of insect pests in cotton.

  15. Diversidade de Carabidae (Coleoptera) Amostrados em Áreas de Reflorestamento de Mata Ciliar e Fragmento Florestal, no Estado do Paraná

    OpenAIRE

    Tamara Quinteiro; José Lopes; Ivan Martins

    2012-01-01

    Carabidae são Coleoptera com a maioria de suas espécies apresentando hábito alimentar predatório. O objetivo do presente trabalho foi estudar a diversidade e abundância da família Carabidae (Coleoptera) em áreas de fragmento florestal e em áreas de reflorestamento de mata ciliar, evidenciando a importância deste grupo como indicador da biodiversidade em relação ao sucesso do reflorestamento. Os besouros foram amostrados por meio de armadilhas de solo pitfall, em fragmentos florestais e reflor...

  16. EFECTO DE LA GLUCOSA Y NITRATO DE AMONIO SOBRE LAS ENZIMAS LIGNINOLÍTICAS PRODUCIDAS POR Trametes versicolor INMOVILIZADO EN ESPUMA Y LA DECOLORACIÓN DE UN EFLUENTE PAPELERO EN UN BIORREACTOR DE LECHO FLUIDIZADO

    Directory of Open Access Journals (Sweden)

    M. Martínez-Salgado

    2005-12-01

    Full Text Available En Colombia se han realizado varios estudios para el tratamiento de aguas residuales de la industria papelera a escala de laboratorio, con el hongo Trametes versicolor inmovilizado en espuma de poliuretano; este hongo ha sido ampliamente estudiado por la producción de enzimas ligninolíticas y su potencial uso en la decoloración de efluentes. En este trabajo, se estudió el efecto de una fuente de carbono (glucosa y una de nitrógeno (nitrato de amonio en un biorreactor de lecho fluidizado, con adición al medio de cultivo de 0.02% (v/v de Tween 80, 12 ppm de MnSO4 y 1 mM de CuSO4, desarrollando un diseño factorial 22 con dos diferentes concentraciones de los nutrientes, siendo éstos las variables independientes y las variables dependientes fueron la actividad enzimática, las unidades de color y el consumo de sustrato los cuales fueron determinados por métodos colorimétricos, además de la biomasa y pH. El uso del biorreactor de lecho fluidizado con entrada de aire constante, arrojó datos importantes sobre la decoloracióny la producción de enzimas en el agua residual no estéril. Los máximos valores de la enzima Mnperoxidasa fueron hallados en las concentraciones altas y bajas de glucosa y nitrato de amonio con valores de 9.257U y 6.502U; para la enzima Lacasa, al adicionar separadamente las dos variables se obtuvo valores de 8.618U y 8.341U. Las enzimas fueron relacionadas con la disminución de las unidades de color hasta 481.06UC, junto con el aumento de la biomasa del hongo Trametes versicolor hasta 3.365 g junto con flora nativa del agua residual. Los resultados encontrados, determinaron que las variables de consumo de sustrato y biomasa, tienen una gran importancia debido a que el aumento de la biomasa genera un aumento en la actividad de la enzima MnP y en la reducción de las unidades de color.

  17. Treatment Effect and Safety of Naftifine/ketoconazole Cream to Facial Pityriasis Versicolor in Children%萘替芬酮康唑乳膏治疗儿童面部花斑癣的疗效观察

    Institute of Scientific and Technical Information of China (English)

    李麟

    2014-01-01

    目的:观察萘替芬酮康唑乳膏治疗儿童面部花斑癣的疗效与安全。方法:将95例患儿随机分成治疗组和对照组,治疗组外用萘替芬酮康唑乳膏,对照组外用特比奈芬软膏,于治疗2、4周后观察疗效及有无不适反应,并根据真菌镜检结果判定疗效。结果:治疗组治疗2周时治愈率为90%,4周时治愈率为100%,不适反应率为10%;对照组治疗2周时治愈率为71%,4周时治愈率为84%,不适反应率为27%。两组有效率及安全性差异有统计学意义(P<0.05)。结论:萘替芬酮康唑乳膏治疗儿童面部花斑癣安全而有效。%Objective:To observe the clinical efficiency and safety in the treatment of facial pityriasis versicolor in children with Naftifine/Ketoconazole Cream. Methods:95 children patients were randomly divided into treatment group and control group. Naftifine/ketoconazole cream were applied in the treatment group, and Terbinafine were applied in the control group. The clinical efficiency and side effects were evaluated 2 and 4 weeks later, and therapeutic effect was determined according to the results of fungus infection.Results:At the end of 2 weeks and 4 weeks, the efficacy of the treatment group was 90%and 100%, while control group was 71%and 84%. The side effects of treatment group was 10%, and control group 27%. All of them had significant difference (P<0.05). Conclusion:Naftifine/ketoconazole cream is efficient and safe to treat facial pityriasis versicolor in the children.

  18. 云芝漆酶的生物降解与染料脱色作用研究%Research on the Biodegradation and Dye Decolourization for Laccase Produced from Trametes versicolor

    Institute of Scientific and Technical Information of China (English)

    姚朝阳; 郭伟云; 牛敬媛; 邵强; 李宗义

    2009-01-01

    [Objective]This research aimed to study the biodegradation and dye decolourization for laccase produced from Trametes versicolor.[Method]Crude laccase from T. versicolor HS-03 was used for the biodegradation of wheat straw and sawdust,and the decolourization of different molecular structure type's dye.[Result]It was indicated that the crude laccase raised the lignocellulose biodegradation efficiency which was about 5-6 doubles.Crude enzyme has wide spectrum decoloured function,especially the decoloring rate of malachite green,RB bright blue was over 85%.The rush hour of decolourization was 48-72 h.[Conclusion]This study could provide theoretical basis for dye decolourization of waste water,and provide strong references for the application of laccase in water treatment industry industrialization.%[目的]研究云芝漆酶的生物降解和染料脱色作用.[方法]以云芝HS-03发酵后所得漆酶粗制品,对麦草和锯末进行生物降解,并对几种常用染料进行脱色研究.[结果]结果表明:漆酶粗制品与纤维素酶、木聚糖酶协同作用大大提高了木质纤维素降解率,比单一组分酶获得还原性糖含量提高5~6倍.漆酶粗制品具有广谱的脱色作用,尤其对孔雀石绿、RB亮蓝的脱色率都超过了85%,脱色较为彻底,脱色高峰期为48~72 h.[结论]该研究可为废水染料脱色提供理论基础,为漆酶在水处理工业产业化中的应用提供强有力的依据.

  19. Locomotor activity of Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae on Chilean sandy beaches Actividad locomotora de Phalerisida maculata Kulzer (Coleoptera, enebrionidae en playas arenosas chilenas

    Directory of Open Access Journals (Sweden)

    EDUARDO JARAMILLO

    2000-03-01

    Full Text Available The locomotor activity of the beetle Phalerisida maculata Kulzer (Coleoptera Tenebrionidae was studied on the surface of the substrate in two sandy beaches of the Chilean coast: one in north central (ca. 29ºS and the other in south central Chile (ca.39ºS. During the summer period of 1991 the circadian locomotor activity was studied in the southern beach, while during that of 1997 in both beaches. To analyze the activity, pitfall traps were used which were ordered along two transects extended between the upper beach and the resurgence zone. The traps were checked (i.e. collection of captured insects every two hours for a total period of 26 hours. The results showed that the adults of P. maculata were mostly active during the night hours, whereas the larvae were active during both, the day and night. Studies carried out in the beach located in south central Chile show that differences in the tidal range (neap vs. spring tides do not affect the activity patterns. During the locomotor activity, adult and larvae move to lower intertidal levels than those usually occupied while buried. Results of laboratory experiments using actographs under conditions of darkness and constant temperature, suggest that adults and larvae of P. maculata presented a circadian rhythm similar to that observed in the field experiments. It is concluded that P. maculata presents a behaviour that appear to be under control of an endogenous rhythm, without showing differences in the circadian rhythm of activity when beaches located at different latitudes are comparedSe estudió la actividad locomotriz del escarabajo Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae sobre la superficie del sustrato de dos playas arenosas de la costa de Chile: una en el centro norte (ca. 29ºS y otra en el centro sur (ca. 39ºS. Durante el período estival de 1991 se estudió la actividad locomotriz circadiana en la playa del centro sur y durante el de 1997 en ambas playas. Para analizar

  20. Tipos de Curculionidae (Coleoptera: Curculionoidea) depositados en la colección entomológica del Museo de La Plata Types of Curculionidae (Coleoptera: Curculionoidea) housed at the Museo de La Plata entomological collection

    OpenAIRE

    Analía A. Lanteri; Suárez, Sonia M.; Guadalupe del Río, M.

    2003-01-01

    Esta contribución referida al material tipo de Curculionidae (Coleoptera: Curculionoidea) depositado en la colección entomológica del Museo de La Plata, actualiza la información sobre los tipos depositados durante los últimos diez años. Los 111 tipos examinados en este trabajo corresponden a 52 especies asignadas a las siguientes subfamilias: Curculioninae (5 spp.), Cryptorhynchinae (8 spp.), Cyclominae (4 spp.), Entiminae (31 spp.), y Erirhininae (4 spp.). Los autores de las especies son C. ...

  1. A new species of oobius trjapitzin (hymenoptera:encyrtidae) from the russian far east that parasitizes eggs of emerald ash borer (coleoptera:buprestidae)

    Science.gov (United States)

    A new egg parasitoid of the emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) from the Russian Far East, Oobius primorskyensis Yao et Duan is described. Both morphological characters and analysis of DNA sequence divergence suggest that this species is different from the previ...

  2. 伊朗北部Golestan省天牛科(鞘翅目)昆虫种类%The species of Cerambycidae (Coleoptera) from Golestan province, northern Iran

    Institute of Scientific and Technical Information of China (English)

    Najmeh SAMIN; Hamid SAKENIN

    2015-01-01

    天牛(鞘翅目Coleoptera:天牛科Cerambycidae)是树木和灌木最重要的害虫.本文记录了伊朗北部Golestan省的天牛种类,总计采集和鉴定了5亚科(Aseminae,Cerambycinae,Lamiinae,Lepturinae和Prioninae)30种天牛.

  3. Effects of exposure to agricultural drainage ditch water on survivorship, distribution, and abundnance of riffle beetles (Coleoptera: Elmidae) in headwater streams of the Cedar Creek watershed, Indiana

    Science.gov (United States)

    Riffle Beetles (Coleoptera: Elmidae) require very good water quality, mature streams with riffle habitat, and high dissolved oxygen content. As such, they prove to be good indicators of ecological health in agricultural headwater streams. We conducted static renewal aquatic bioassays using water fro...

  4. Assembly and annotation of full mitochondrial genomes for the corn rootworm species, Diabrotica virgifera virgifera and D. barberi (Insecta: Coleoptera: Chrysomelidae), using Next Generation Sequence data

    Science.gov (United States)

    Complete mitochondrial genomes for two corn rootworm species, Diabrotica v. virgifera (16,747 bp) and D. barberi (16,632; Insecta: Coleoptera: Chrysomelidae), were assembled from Illumina HiSeq2000 read data. Annotation indicated that the order and orientation of 13 protein coding genes (PCGs), and...

  5. Descrição da larva de último instar e pupa de Epacroplon cruciatum (Aurivillius (Coleoptera, Cerambycidae, Cerambycinae e notas biológica

    Directory of Open Access Journals (Sweden)

    Juares Fuhrmann

    2012-03-01

    Full Text Available Descrição da larva de último instar e pupa de Epacroplon cruciatum (Aurivillius (Coleoptera, Cerambycidae, Cerambycinae e notas biológicas. Último instar larval e pupa do Hexoplonini sul-americano Epacroplon cruciatum (Aurivillius, 1899 são descritos, ilustrados e disponibilizadas notas biológicas.

  6. Immunofluorescence localization and ultrastructure of Stewart’s wilt disease bacterium Pantoea stewartii in maize leaves and in its flea beetle vector Chaetocnema pulicaria (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Pantoea stewartii is the causal agent of Stewart's wilt of sweet corn, the most serious bacterial disease of sweet corn and maize in the North-Central and Eastern USA. P. stewartii is transmitted mainly by the corn flea beetle Chaetocnema pulicaria (Coleoptera: Chrysomelidae) and this bacterium is a...

  7. Brood production by Xylosandrus germanus (Coleoptera: Curculionidae) and growth of its fungal symbiont on artificial diet based on sawdust of different species

    Science.gov (United States)

    The ambrosia beetle Xylosandrus germanus (Blanford) (Coleoptera: Curculionidae) is among the most important exotic pests of orchards and nurseries in the US. It attacks a wide range of hosts and is difficult to control using conventional insecticides. As part of our studies on the biology and cont...

  8. Trapping sweetpotato weevil, Cylas formicarius elegantulus (Coleoptera: Brentidae), with high doses of sex pheromone: Catch enhancement and weathering rate in Hawaii

    Science.gov (United States)

    Sweetpotato, Ipomoea batatas (L.) Lamarck, one of the top ten staple crops produced worldwide, has increased in production in Hawaii in recent years. The sweetpotato weevil, Cylas formicarius elegantulus (Summers)(Coleoptera: Brentidae), is a major economic and quarantine pest of sweetpotato in Hawa...

  9. Post entry interception of the yellow-spotted longhorned beetle,Psacothea hilaris (Coleoptera: Cerambycidae) in Italy Genbank Accession Number GU244486

    Science.gov (United States)

    Psacothea hilaris (Pascoe) (Coleoptera: Cerambycidae) known as the yellow-spotted longhorned beetle is native from eastern Asia (China, Japan including Ryukyu, Shikoku and Honshu archipelago and Taïwan) where it attacks plants belonging to Moraceae family, in particular to Morus and Ficus genera. In...

  10. Biology of two members of the Euwallacea fornicatus species complex (Coleoptera: Curculionidae: Scolytinae), recently invasive in the U.S.A., reared on an ambrosia beetle artificial diet

    Science.gov (United States)

    1. Diet and rearing protocols were developed for two members of the cryptic Euwallacea fornicatus species complex, polyphagous shot hole borer (PSHB) and tea shot hole borer (TSHB) (Coleoptera: Curculionidae: Scolytinae), using sawdust from boxelder Acer negundo and avocado Persea americana. 2. Bio...

  11. New records of Paracrias Ashmead (Hymenoptera, Eulophidae as parasitoids on weevil larvae (Coleoptera, Curculionidae in Brazil, with the description of a new species

    Directory of Open Access Journals (Sweden)

    Luciano Palmieri

    2013-09-01

    Full Text Available Paracrias strii Schauff, 1985 and P. ceratophaga Palmieri & Hansson sp. nov. are first record in Brazil and both are associated with Ceratopus Schoenherr larvae (Coleoptera, Curculionidae reared from syconia of two species of fig-trees. Both Paracrias species are diagnosed and illustrated. Males of P. ceratophaga sp. nov. are described. The association of Paracrias with weevil larvae is briefly discussed.

  12. Current Knowledge of Mesozoic Coleoptera from Daohugou and Liaoning (Northeast China)

    Institute of Scientific and Technical Information of China (English)

    Alexander G.KIREJTSHUK; Alexander G.PONOMARENKO; Alexander A.PROKIN; CHANG Huali; Georgy V.NIKOLAJEV; REN Dang

    2010-01-01

    The present paper is devoted to an overview on fossil Coleoptera studied from Inner Mongolia,Daohugou (Middle Jurassic,Jiulongshan Formation) and Liaoning (Upper Jurassic-Lower Cretaceous,Yixian Formation) deposited in Chinese collections.As a result,species of the tribe Sperchopsini and Hydrophilini from Hydrophilidae,families and subfamilies Silphidae,Syndesinae from Lucanidae,Pleocomidae,Trogidae,Trogissitidae,Pyrochroidae,Diaperinae from Tenebrionidae,and Cerambycidae were first registered in the Mesozoic and some families were defined as new.It was shown that many superfamilies represented in the Recent Fauna were formed within the Middle Jurassic and Lower Cretaceous.The materials examined confirm the hypothesis that Cucujiformian beetles are a younger group than other infraordera of Polyphaga (Staphyliniformia and Elateriformia) and,therefore,they appeared in the fossil record only in the late Mesozoic.It was shown and confirmed that most superfamilies appeared in the fossil records before Cucujoidea.The synonymy of Notocupes Ponomarenko,1964;Sinocupes Lin,1976,syn.nov.;Amblomma Tan,Ren et Liu 2005,syn.nov.;Euryomma Tan,Ren et Shih,2006,syn.nov.,non Stein,1899 and Ovatocupes Tan et Ren,2006,syn.nov.;synonymy of Tetraphalerus Waterhouse,1901 and Odontomma Tan,Ren et Ge 2006,syn.nov.;and synonymy of Priacmopsis Ponomarenko,1966 and Latocupes Tan et Ren,2006,syn.nov.are proposed.Sinorhombocoleus papposus Tan et Ren,2009 is transferred from the family Rhombocoleidae to Schizophoridae.Cervicatinius complanus Tan,Ren et Shih,2007 and Forticatinius elegans Tan,Ren et Shih,2007 are transferred from the family Catiniidae (suborder Archostemata) to superfamily Cleroidea (suborder Polyphaga:first among the family Peltidae and second as a closely related group to the latter family).The family Parandrexidae is transferred from the superfamily Cucujoidea to Cieroidea.The ecological circumstances of the past ecosystems and hypotheses of historical development of the order

  13. Avaliação de inseticidas no controle de Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae e Rhyzopertha dominica (Fabricius (Coleoptera, Bostrichidae em trigo armazenado Efficacy of insecticides to control Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae and Rhyzopertha dominica (Fabricius (Coleoptera, Bostrichidae in stored wheat

    Directory of Open Access Journals (Sweden)

    Rui S. Furiatti

    1999-03-01

    Full Text Available The efficiency of insecticides against Sitophilus oryzae (Linnaeus, 1763 (Coleoptera, Curculionidae and Rhyzopertha dominica (Fabricius, 1792 (Coleoptera, Bostrichidae was determined exposing them to treated wheat in laboratory. Each plot, which was composed by 1.2 kg of wheat grain, was treated with 5 ml of the concentration/kg of wheat using a propelling air atomizer. Fifteen days after treatment 50 g samples of the grain were taken, from each plot, placed in plastic jars into which 10 adult insects of each species were released. This was repeated every 30 days until 165 days after treatment. The number of dead insects was counted 15 days after each infestation thus the evaluations were made at 30, 60, 90, 120, 150 and 180 days after the insecticide application. Esfenvalerate+fenitrothion+pyperonil butoxide (0,375+7,5+3,0 and 0,5+10,0+4,0 g a.i./t, fenitrothion+esfen-valerate (7,5+0,375 and 10,0+0,375 g a.i./t, fenitrothion+synergised deltamethrin (7,5+0,375 g a.i./t and fenitrothion (7,5 g a.i./t were effective against Sitophilus oryzae until 180 days after treatment. Esfenvalerate+fenitrothion+pyperonil butoxide (0,5+10,0+4,0 g a.i./t, fenitrothion+esfenvalerate (7,5+0,375 and 10,0+0,375 g a.i./t, fenitrothion+synergised deltamethrin (7,5+0,375 g a.i./t were effective against R. dominica until 180 days after treatment. Synergised deltamethrin (0,375 g a.i./t was effective against S. oryzae until 150 days and against R. dominica until 120 days after treatment. Esfenvalerate (0,5 g a.i./t was ineffective against S. oryzae and effective until 60 days after treatment against R. dominica. Fenitrothion (7,5 g a.i./t was ineffective against R. dominica. The use of mixtures of organophosphorus plus pyrethroids were more effective against S. oryzae and R. dominica when compared with these insecticides used alone.

  14. Isolation,Purification and Structural Characterization of an Aqueous Polysacc-haride from Natural Coriolus versicolor%野生云芝中水溶性多糖的分离鉴定

    Institute of Scientific and Technical Information of China (English)

    陈海生; 梁荣能; 徐一新

    2000-01-01

    目的:进一步研究野生云芝(Coriolus versicolor (L.) Fr.)子实体中具有生物活性的多糖成分.方法:采用水提醇沉淀法得粗多糖,经H2O2脱色,醇分部沉淀,SephadexG75和HPLC纯化,GC和光谱测定糖组成及糖链结构.结果:从野生云芝中分离纯化了一种水溶性多糖CVPS-B,分子量39kd,其单糖组成及摩尔比为:Glc,Fuc,Ara,Man,Gal,13.647∶5.882∶4.452:2.425∶1.000;其多糖主链为β(1-3)葡聚糖,支链上含有一定量的夫糖(fucose).结论:CVPS-B为云芝中新发现的一组分多糖,与日本培养的云芝多糖PS-K类似,但不含多肽,而与上海师范大学培养的云芝多糖PSP有所不同.

  15. Coleoptera and microbe biomass in Antarctic Dry Valley paleosols adjacent to the Inland Ice: Implications for Mars

    Science.gov (United States)

    Mahaney, William C.; Hart, Kris M.; O'Reilly, Shane S.; Allen, Christopher C. R.; Dohm, James M.; Hancock, Ronald G. V.; Kelleher, Brian P.; Milner, Michael W.

    2012-01-01

    Bulk paleosol samples collected from a Middle to Early Miocene moraine in the New Mountain area of the Dry Valleys, Antarctica, yielded Coleoptera exoskeletons and occasional endoskeletons showing considerable diagenetic effects along with several species of bacteria, all lodged in a dry-frozen but salt-rich horizon at shallow depth to the land surface. The till is at the older end of a chronologic sequence of glacial deposits, thought to have been deposited before the transition from wet-based to cold-based ice (∼15 Ma), and hence, entirely weathered in contact with the subaerial atmosphere. It is possible, though not absolutely verifiable, that the skeletons date from this early stage of emplacement having undergone modifications whenever light snowmelt occurred or salt concentrations lowered the freezing temperature to maintain water as liquid. Correlation of the Coleoptera species with cultured bacteria in the sample and the likelihood of co-habitation with Beauveria bassiani found in two adjacent, although younger paleosols, leads to new questions about the antiquity of the Coleoptera and the source of N and glucose from chitinase derived from the insects. The skeletons in the 831 section may date close to the oldest preserved chitin (Oligocene) yet found on Earth. While harsh Martian conditions make it seemingly intolerable for complex, multicellular organisms such as insects to exist in the near-surface and subaerially, life within similar cold, dry paleosol microenvironments (Cryosols) of Antarctica point to life potential for the Red Planet, especially when considering the relatively diverse microbe (bacteria and fungi) population.

  16. Seasonal dynamics of saproxylic beetles (Coleoptera occurring in decaying birch (Betula spp. wood in the Kampinos National Park

    Directory of Open Access Journals (Sweden)

    Sawoniewicz Michał

    2015-09-01

    Full Text Available The aim of the study was to identify the seasonal changes in the number of saproxylic beetles connected with birch in the Kampinos National Park. The research was conducted for 12 consecutive months in research areas representing 10 different site types. The beetles were collected from wood using photoeclectors. The largest number of species was collected in April and the lowest in January. An increase in number occurred during spring and summer months for species associated only with rotting wood, fructifications of tree fungi, the subcortical environment and hollows. In the same period the number of species not associated or potentially associated with decaying trees and wood decreased. During winter months, the differences in the number of trapped specimens were the smallest. The proportion of zoophagous species amongst the collected specimen increased in autumn and winter. The share of saprophagous species was the highest during the summer-autumn period and the share of mycetophages (jointly with myxomycophages was the highest during spring and summer. We distinguished two separate groups of Coleoptera with the first one (‘summer group’ including species trapped during late-spring and summer months, while the second one (‘winter group’ includes species found in autumn, winter and early-spring months. In the ‘summer group’, an average of 55.8 species was trapped each month with 331.2 specimen of Coleoptera, while in the ʻwinter group’ an average of 56.1 species with 228.4 Coleoptera specimen were caught.

  17. Redefinition of the genus Silphitrombium (Trombidiformes: Neothrombiidae) with description of two new species parasitizing beetles (Coleoptera: Elateridae, Tenebrionidae) from Iran.

    Science.gov (United States)

    Tashakor, Samaneh; Hajiqanbar, Hamidreza; Saboori, Alireza

    2013-11-15

    Two new species of Silphitrombium Fain, 1992 (Acari: Prostigmata: Neothrombiidae), ectoparasites of beetles (Insecta: Coleoptera), are described from Sistan and Baluchestan Province, eastern Iran: S. elateridum sp. nov. on Heteroderes heideni Reitter, 1891 (Col.: Elateridae) and S. iranicum sp. nov. on Opatroides punctulatus Brullé, 1832 (Col.: Tenebrionidae) and the genus Silphitrombium is redefined. It is the first record of the relationship between beetles of the families Elateridae and Tenebrionidae, and mites of the genus Silphitrombium. A key to the species of the genus is presented.

  18. Sucinolivolia torpida--a new genus and species of flea-beetles (Coleoptera: Chrysomelidae: Galerucinae) from Baltic amber.

    Science.gov (United States)

    Bukejs, Andris; Biondi, Maurizio; Alekseev, Vitalii I

    2015-12-15

    Sucinolivolia torpida gen. nov. et sp. nov. (Coleoptera: Chrysomelidae: Galerucinae: Alticini) is described and illustrated from Eocene Baltic amber. The new monotypic genus is compared with fossil and extant flea-beetle genera. Sucinolivolia gen. nov. is similar to the extant Livolia Jacoby and Orthaltica Crotch, but difference include the absence of an antebasal pronotal sulcus, not crenulate lateral pronotal margins, possessing very short genae, more robust legs, and the shape of tibiae. Including this new record, six described species of Alticini are known from Baltic amber.

  19. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    Abstract A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  20. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus.

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  1. Effect of temperature on reproduction and embryonic development of the cabbage stem flea beetle, Psylliodes chrysocephala L., (Coleoptera: Chrysomelidae)

    DEFF Research Database (Denmark)

    Mathiasen, Helle; Sørensen, Helle; Bligaard, J.;

    2015-01-01

    The cabbage stem flea beetle, Psylliodes chrysocephala (L.) (Coleoptera: Chrysomelidae), is a major pest of winter oilseed rape. Despite the importance of this pest, detailed information on reproduction to predict risk of crop damage is lacking. This study investigates the effect of temperature...... on parameters of reproduction, egg development and viability at five constant temperatures. Significant temperature effects were found on the pre-oviposition period, total number of eggs laid, daily oviposition rate, female longevity, egg-development rate and viability. The mean length of the pre...

  2. Influence of 60Co γ irradiation on fertility of Japanese pine sawyer beetle Monochamus alternatus hope (Coleoptera: Cerambycidae)

    International Nuclear Information System (INIS)

    The fertility of the Japanese pine sawyer beetle Monochamus alternatus (Coleoptera: Cerambycidae) irradiated with 60Co γ-rays was remarkable reduced at the doses of 30Gy, 35Gy and 40Gy, especially at 40Gy. When the non-irradiated females were coupled with the irradiated males first, and then coupled with non-irradiated males, the hatchability and the fertility had little higher but lower than the control. It explained that radiation has certain influence to the female gonad. It also has difference between the hatching rate and the amount of eggs in different match. (authors)

  3. Registros y distribución de la especie afroasiática Digitonthophagus gazella (Coleoptera: Scarabaeidae: Scarabaeinae en Argentina

    Directory of Open Access Journals (Sweden)

    María C. ÁLVAREZ BOHLE

    2009-01-01

    Full Text Available Se cita por primera vez el escarabajo coprófago Digitonthophagus gazella Fabricius (Coleoptera: Scarabaeidae para Argentina, por lo que se amplía su área de distribución geográfica en Sudamérica. Esta especie de origen afro-asiático fue capturada mediante colecta manual en estiércol de ganado bovino y equino, con trampas de luz y trampas de caída cebadas con estiércol en seis provincias del centro y norte argentino entre los años 2006 y 2009.

  4. Ambrosia Beetle (Coleoptera: Scolytidae) Species, Flight, and Attack on Living Eastern Cottonwood Trees.

    Energy Technology Data Exchange (ETDEWEB)

    Coyle, D R; D.C. Booth: M.S. Wallace

    2005-12-01

    ABSTRACT In spring 2002, ambrosia beetles (Coleoptera: Scolytidae) infested an intensively managed 22-ha tree plantation on the upper coastal plain of South Carolina. Nearly 3,500 scolytids representing 28 species were captured in ethanol-baited traps from 18 June 2002 to 18 April 2004. More than 88% of total captures were exotic species. Five species [Dryoxylon onoharaensum (Murayama), Euwallacea validus (Eichhoff), Pseudopityophthorus minutissimus (Zimmermann), Xyleborus atratus Eichhoff, and Xyleborus impressus Eichhoff]) were collected in South Carolina for the first time. Of four tree species in the plantation, eastern cottonwood, Populus deltoides Bartram, was the only one attacked, with nearly 40% of the trees sustaining ambrosia beetle damage. Clone ST66 sustained more damage than clone S7C15. ST66 trees receiving fertilization were attacked more frequently than trees receiving irrigation, irrigation_fertilization, or controls, although the number of S7C15 trees attacked did not differ among treatments. The study location is near major shipping ports; our results demonstrate the necessity for intensive monitoring programs to determine the arrival, spread, ecology, and impact of exotic scolytids.

  5. ATIVIDADE INSETICIDA DE PLANTAS MEDICINAIS SOBRE O Callosobruchus maculatus (COLEOPTERA: BRUCHIDAE

    Directory of Open Access Journals (Sweden)

    Antonia Mirian Nogueira de Moura Guerra

    2009-01-01

    Full Text Available This study aimed to evaluate the insecticidal activity of eight medicinal plants on Callosobruchus maculatus (F. (Coleoptera: Bruchidae. Plant powder from Rosmarinus officinalis (L. leaves, Peumus boldus (Mol leaves, Matricaria chamomilla (L. flowers, Baccharis trimera (Less. leaves, Camellia sinensis (L. leaves, Thea sinensis (L. leaves, Ilex paraguariensis (St. Hil. leaves, and fruits of Pimpinella anisum (L. were used in the experiment. Bioassays were carried out under constant conditions of temperature (28±2 °C, relative humidity (70±5% and scotophase of 24 hours. Experimental units consisted of 20 grams of cowpea (Vigna unguiculata (L. Walp. grains with 13% wet basis moisture content, 2 g plant powder and ten adult insects, both placed in plastic containers (60 mm in diameter x 60 mm high. The analyzed variables included: insect mortality, oviposition (egg number and instantaneous rate of population growth (ri. The number of dead insects was recorded 5 days after bioassays were started, the egg number was recorded after 12 days, and ri was estimated on the 60th day. All the tested plants had toxicity to C. maculatus. The highest mortality was found in insects treated with P. boldus and I. paraguariensis. Number of eggs and ri also varied significantly among the powders. The lowest mean values for these parameters were recorded in the plots treated with P. boldus, P. anisum and R. officinalis.

  6. Fumigation Toxicity of Essential Oil Monoterpenes to Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae

    Directory of Open Access Journals (Sweden)

    Olufunmilayo E. Ajayi

    2014-01-01

    Full Text Available The fumigant toxicity of eight essential oil components, 1-8-cineole, carvacrol, eugenol, (−-menthone, (−-linalool, S-(−-limonene, (−-β-pinene, and (+-α-pinene, was tested against the cowpea weevil, Callosobruchus maculatus (Fabricius (Coleoptera: Chrysomelidae, at 0.25–60 µL/L air doses. 1-8-Cineole, carvacrol, and eugenol caused complete adult mortality at 10 µL/L air 24 h after treatment. 1-8-Cineole and carvacrol were the most toxic with LD50 values of 0.24 and 0.6 µL/L air at 24 h, respectively. (−-β-Pinene and (+-α-pinene were the least toxic with LD50 values of 31 and 31.4 µL/L air at 24 h, respectively. Toxicity was negatively correlated with vapor pressure. 1-8-Cineole and carvacrol caused 100% oviposition deterrence at all doses tested. Eugenol and (−-menthone completely inhibited adult emergence. S-(−-Limonene, (−-β-pinene, and (+-α-pinene were not effective at preventing oviposition or adult emergence, suggesting that a lethal dose of the three oil components would be necessary to control C. maculatus infestations.

  7. Sustainable management tactics for control of Phyllotreta cruciferae (Coleoptera: Chrysomelidae) on canola in Montana.

    Science.gov (United States)

    Reddy, Gadi V P; Tangtrakulwanich, Khanobporn; Miller, John H; Ophus, Victoria L; Prewett, Julie

    2014-04-01

    The crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae), has recently emerged as a serious pest of canola (Brassica napus L.) in Montana. The adult beetles feed on canola leaves, causing many small holes that stunt growth and reduce yield. In 2013, damage to canola seedlings was high (approximately 80%) in many parts of Montana, evidence that when flea beetles emerge in large numbers, they can quickly destroy a young canola crop. In the current study, the effectiveness of several biopesticides was evaluated and compared with two insecticides (deltamethrin and bifenthrin) commonly used as foliar sprays as well as seed treatment with an imidacloprid insecticide for the control of P. cruciferae under field conditions in 2013. The biopesticides used included an entomopathogenic nematode (Steinernema carpocapsae), two entomopathogenic fungi (Beauveria bassiana and Metarhizium brunneum), neem, and petroleum spray oils. The control agents were delivered in combination or alone in a single or repeated applications at different times. The plant-derived compound neem (azadirachtin), petroleum spray oil, and fatty acids (M-Pede) only showed moderate effect, although they significantly reduced leaf injuries caused by P. cruciferae and resulted in higher canola yield than the untreated control. Combined use of B. bassiana and M. brunneum in two repeated applications and bifenthrin in five applications were most effective in reducing feeding injuries and improving yield levels at both trial locations. This indicates that entomopathogenic fungi are effective against P. cruciferae, and may serve as alternatives to conventional insecticides or seed treatments in managing this pest. PMID:24772547

  8. Transcriptomic Analysis of Eucryptorrhynchus chinensis (Coleoptera: Curculionidae) Using 454 Pyrosequencing Technology.

    Science.gov (United States)

    Liu, Zhen-Kai; Wen, Jun-Bao

    2016-01-01

    Eucryptorrhynchus chinensis Olivier (Coleoptera: Curculionidae) is one of the most important pests of Ailanthus altissima; however, so far, no studies on the genome or transcriptome of E. chinensis have been reported. Using the Roche 454 FLX Titanium platform, an RNA pool obtained from E. chinensis eggs, larva, pupae, and adults was sequenced and assembled de novo to achieve maximum diversity of sampled transcripts. We obtained 1,441,137 (∼518 Mb) raw reads with an average length of 360 bp. After trimming, 89% qualified reads were produced and assembled into 35,509 isotigs with an average length of 440 bp, N50 of 1,048 bp, and 111,643 singletons. We generated 87,894 unigenes following a cluster analysis of the isotigs and singletons, and then functionally annotated the unigenes with gene descriptions. We obtained 23,363 GO assignments, and 12,724 unigenes were assigned to KOG. Based on these annotations, 294 biochemical pathways involved in growth, reproduction, and stress or immune responses were predicted. A total of 659,026 single nucleotide variants and 6,112 simple sequence repeats were detected. Our data provide comprehensive information on the sequence and possible functions of E. chinensis transcripts. PMID:27620556

  9. Annotated catalogue of the carabid beetles of the Republic of Macedonia (Coleoptera: Carabidae).

    Science.gov (United States)

    Hristovski, Slavčo; Guéorguiev, Borislav

    2015-08-20

    The catalogue of the ground beetles (Coleoptera: Carabidae) of the Republic of Macedonia is the result of our permanent investigation during 15 years. It is based on the critical review of the data in 255 scientific publications and the revision of the collections deposited in the museums in Macedonia (Skopje and Struga), other European countries (Berlin, Budapest, Vienna, Sofia) and the first author's private collection. For all of the species and subspecies we have presented the known literature references, precise data for the studied material and overall distribution in the Republic of Macedonia. The study of the material resulted in new country records of 10 genera, 101 species and 25 subspecies. First detailed records are provided for another 47 species and subspecies, and additional material was studied of 482 species and subspecies. Type material of 18 species and subspecies was also examined. Thirteen species and one subspecies were rejected from the list of Macedonian ground beetles. Six more species are treated as questionable and were not included in the present list. As a result, the presence of 571 species and 234 subspecies (626 taxa in total) in Macedonia is confirmed. These taxa are arranged in 104 genera, 31 subtribes, 35 tribes and 13 subfamilies. The most numerous in term of the species are the genera Bembidion (60), Harpalus (48) and Amara (46), as well as Pterostichus (26), Ophonus (19), Carabus (16), Trechus (16), Brachinus (16) and Dyschirius (15).

  10. Treatment outcome of Paederus dermatitis due to rove beetles (Coleoptera: Staphylinidae) on guinea pigs.

    Science.gov (United States)

    Fakoorziba, M R; Eghbal, F; Azizi, K; Moemenbellah-Fard, M D

    2011-08-01

    Linear dermatitis (or dermatitis linearis, DL) is a skin blistering inflammatory lesion caused by exposure to the pederin toxin from rove beetles. Although it is prevalent in many countries of the Middle East region, this is not a notifiable disease. In recent years, a number of clinical symptoms outbreaks of DL has been reported from a few neighboring countries of Iran, but no report of experimental treatment among small laboratory rodents is known. This is a prerequisite to ascertain the nature of the best treatment strategy in cases of infestation with these beetles, as it occurs among local settlers during hot seasons in certain parts of the southern Iranian province of Fars. Live Paederus beetles were collected, identified to species level, sexed apart and partly processed to obtain their hemolymph toxin pederin in ethanol for dermal application on guinea pigs. Two Paederus species were found. Paederus ilsae (Bernhauer) (Coleoptera: Staphylinidae) was more abundant than P. iliensis (Coiffait). Recovery from DL due to live P. ilsae beetles was quicker and less complex than that of pederin in ethanol on guinea pigs. The application of potassium permanganate with calamine to heal DL was also more effective than fluocinolone treatment. This topical corticosteroid is thus considered less able to avert the cytotoxic action of pederin on the skin of guinea pigs than the antipruritic and cleansing agents. It seems likely that fluocinolone has certain effects which delays the recovery period for the treated skin.

  11. Stenusine, an antimicrobial agent in the rove beetle genus Stenus (Coleoptera, Staphylinidae)

    Science.gov (United States)

    Lusebrink, Inka; Dettner, Konrad; Seifert, Karlheinz

    2008-08-01

    Stenusine is well known as the alkaloid, discharged by the rove beetle, genus Stenus Latreille (Coleoptera, Staphylinidae). The Stenus beetles employ the alkaloid as an escape mechanism when on water surfaces. In the case of danger, they lower their abdomen and emit stenusine from their pygidial glands. Stenusine shows a low surface tension and therefore a high spreading pressure; these properties propel the beetle quickly over the water. Many Steninae do not live in habitats with open waters, but in detritus, leaf litter, mosses, etc. This raises the possibility that stenusine might also have another function, e.g., as antibiotic or fungicide. Stenus beetles show an intense grooming behaviour. With gas chromatography mass spectrometry analyses we could prove that they cover themselves with their secretion. To tests its antimicrobial properties we conducted agar diffusion tests with stenusine and norstenusine, another substance that is abundant in most Stenus species. Both compounds have an antimicrobial effect on entomopathogenic bacteria and fungi. Stenusine not only allows for an extraordinary method of locomotion on water surfaces, it also protects the Steninae from being infested with microorganisms.

  12. Identification, distribution, and adult phenology of the carrion beetles (Coleoptera: Silphidae) of Texas.

    Science.gov (United States)

    Mullins, Patricia L; Riley, Edward G; Oswald, John D

    2013-01-01

    The carrion beetles (Coleoptera: Silphidae) of Texas are surveyed. Thirteen of the 14 species, and five of the six genera, of this ecologically and forensically important group of scavengers that have previously been reported from Texas are confirmed here based on a study of 3,732 adult specimens. The one reported, but unconfirmed, species, Oxelytrum discicolle, was probably based on erroneous label data and is excluded from the Texas fauna. Two additional species, Nicrophorus sayi and N. investigator are discussed as possible, but unconfirmed, components of the fauna. Taxonomic diagnoses, Texas distribution range maps, seasonality profiles, and biological notes are presented for each confirmed species. The confirmed Texas silphid fauna of 13 species comprises 43% of the 30 species of this family that are known from America north of Mexico. The highest richness (11 species) is found in the combined Austroriparian and Texan biotic provinces of eastern Texas. Phenologically, three species (Necrophila americana, O. rugulosum and Nicrophorus tomentosus) exhibit bimodal adult temporal occurrence patterns with peaks in the spring and late summer or fall; four species (Oiceoptoma noveboracense, Necrodes surinamensis, Nicrophorus carolinus, and N. orbicollis) exhibit unimodal occurrence patterns with a single peak in late spring or early summer; one species (Oiceoptonia inaequale) exhibits a unimodal occurrence pattern with a single peak in early spring; and five species (Thanatophilus truncatus, Nicrophorus americanus, N. marginatus, N. mexicanus and N. pustulatus) display unimodal occurrence patterns with peaks in late summer or early fall.

  13. Fossil mesostigmatid mites (Mesostigmata: Gamasina, Microgyniina, Uropodina), associated with longhorn beetles (Coleoptera: Cerambycidae) in Baltic amber

    Science.gov (United States)

    Dunlop, Jason A.; Kontschán, Jenő; Zwanzig, Michael

    2013-04-01

    Fossil mesostigmatid mites are extremely rare. Inclusions assignable to the tortoise mites (Mesostigmata, Uropodina) are described here for the first time from Eocene (ca. 44-49 Ma) Baltic amber. This is the oldest record of Uropodina and documents the first unequivocal amber examples potentially assignable to the extant genus Uroobovella Berlese, 1903 (Uropodoidea: Urodinychidae). Further mites in the same amber pieces are tentatively assigned to Microgynioidea (Microgyniina) and Ascidae (Gamasina), both potentially representing the oldest records of their respective superfamily and family groups. This new material also preserves behavioural ecology in the form of phoretic deutonymphs attached to their carriers via a characteristic anal pedicel. These deutonymphs in amber are intimately associated with longhorn beetles (Coleoptera: Cerambycidae), probably belonging to the extinct species Nothorhina granulicollis Zang, 1905. Modern uropodines have been recorded phoretic on species belonging to several beetle families, including records of living Uroobovella spp. occurring on longhorn beetles. Through these amber inclusions, a uropodine-cerambycid association can now be dated back to at least the Eocene.

  14. Artificial substrates for oviposition and larval development of the pepper weevil (Coleoptera: Curculionidae).

    Science.gov (United States)

    Addesso, K M; McAuslane, H J; Stansly, P A; Slansky, F; Schuster, D J

    2009-02-01

    The pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae), is a major pest of cultivated peppers (Capsicum spp.) and other cultivated and wild species within the family Solanaceae. Laboratory study of this insect, as well as its biological control agents, will be greatly facilitated by an artificial rearing system that does not rely on pepper fruit. An egg collection method and amendments to a standard larval diet were investigated for use in the rearing of this weevil. Spherical sachets made of Parafilm or netting enclosing leaves of pepper, American black nightshade, eggplant, tomato, potato, and jasmine tobacco induced oviposition. Tomato, potato, and jasmine tobacco leaves were accepted despite the fact that these are not oviposition hosts for pepper weevils in the wild. A standard larval diet formula was modified in an attempt to improve egg hatch, larval survival, developmental time, and adult mass. The diet formula was modified with the addition of freeze-dried jalapeño pepper powder, an additional lipid source, alternate protein sources, and the removal of methyl paraben. None of the aforementioned treatments resulted in a significant improvement over the standard diet. Egg hatch was greater when eggs were incubated on moist paper towels rather than in diet; thus, placement of neonates rather than eggs into diet improved production of adults. Suggestions for more efficient rearing of weevils on the currently available diet and future directions for the development of an artificial rearing system for pepper weevil are discussed.

  15. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases.

    Science.gov (United States)

    Downey, Danielle; Chun, Stacey; Follett, Peter

    2015-06-01

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. PMID:26470205

  16. Life History of the Tamarind Weevil, Sitophilus linearis (Herbst (Coleoptera: Curculionidae, on Tamarind Seed

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    James Adebayo Ojo

    2015-01-01

    Full Text Available The tamarind weevil, Sitophilus linearis Herbst (Coleoptera: Curculionidae, is an important pest of tamarind and other Caesalpinioideae. Investigating its life history is important in the implementation of management strategy. Its life history was monitored daily to understand its developmental biology on tamarind seed following standard procedures under laboratory conditions of 24–30°C temperature, 60–70% relative humidity, and 12L : 12D photoperiod. The egg incubation period lasted 3.17 ± 0.07 days. A mated female of S. linearis laid an average of 165 ± 5.78 eggs during an oviposition period of 86.8 ± 2.47 days. There were four larval instars, with a total larval developmental period of 16 days. The pupal period lasted 8 days, and adult lived 108.5 ± 3.61 days. The overall growth ratio for the four instars was 1.33. There was a regular relationship and significant correlation (r=0.94 between the stages of larval development and head capsule width.

  17. Preliminary Diversity Of Dung Beetles (Coleoptera: Scarabaeinae Attracting By Human Dung (Calvario, Meta

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    Ruth Mariela Castillo Morales

    2011-12-01

    Full Text Available In the Private Reserve, Refugio del Oso de Anteojos (El Calvario-Meta, the diversity of dung beetles was studied (Coleoptera: Scarabaeinae, grouped around different types of vegetation (Montane Rain Forest, Rainforest Premontane, Lower Montane Rain Forest, by using pitfall traps baited with human dung. During the samplings carried out in the rainy station on 2008, were captured a total of 177 individuals belonging to 9 species. The tribe Canthonini was the most representative with 87.5%, of the described species, followed by Aechini 10.8%, and Onthophagini 1.7%. The dominant species was Canthon lituratus in the three vegetation units: Lower montane rain forest was the richest (9 species and abundance (86 individuals, compared with other environments sampled. By calculating the complementarity index, shows a similar species composition in the three habitats sampled, possibly, related to the floristic composition presented in each one of them.

  18. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae

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    Andréa Roveré Franz

    2011-03-01

    Full Text Available Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae. Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf, Zingiber officinale (root and Mentha sp. (leaf. The efficacy of these oils was tested to control the rice weevil, S. oryzae, using hydrodistillation. Chemical analysis of the essential oils was carried out by gas chromatography. Major components of C. citratus were geranial (48% and neral (31%, of Z. officinale were α-zingibereno (13%, geranial (16%, neral (10% and α-farneseno (5% and of Mentha sp. was menthol (92%. Bioassays were carried out by fumigation and topical application. In topical application assays, the essential oil of C. citratus had greater toxicity (LC50 0.027 µL mL-1 and shorter exposure time than the oils of the other two plants. After 24 h and 48 h, 70% and 100% mortality of S. oryzae occurred, respectively. In fumigation assays, essential oil of Z. officinale had a lower LC50 (1.18 µL cm-2 and 70% mortality after 24 h exposure. Therefore, we recommend the use of essential oils of C. citratus and Z. officinale to control the rice weevil S. oryzae.

  19. Characterization of the complete mitochondrial genome of the firefly, Luciola substriata (Coleoptera: Lampyridae).

    Science.gov (United States)

    Mu, Feng-Juan; Ao, Liang; Zhao, Hua-Bin; Wang, Kai

    2016-09-01

    The firefly, Luciola substriata (Coleoptera: Lampyridae), is an aquatic firefly species, whose larvae inhabit ponds or lakes. Here we present the complete mitochondrial (mt) genome of the firefly (GenBank accession number KP313820) and provide its annotation. This circular genome is 16,248 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a non-coding AT-rich region. Similar to other firefly species, the base composition of this mitochondrial genome is also biased toward A and T (44.09% A, 34.00% T, 12.89% C, and 9.01% G). All 13 protein-coding genes start with a typical mitochondrial start codon, and terminate with a usual stop codon TAA, or TAG or a single T. The non-coding AT-rich region (1636 bp in length) include one (A)20, and two (T)15 tandem repeats, and one (AAT)5 element. This mitochondrial genome sequence will promote a better understanding for firefly evolution in the future. PMID:25714154

  20. Development and Life History of Sitophilus zeamais (Coleoptera: Curculionidae on Cereal Crops

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    James Adebayo Ojo

    2016-01-01

    Full Text Available The maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae, is one of the most destructive pests of stored cereals. Knowledge of the life history and biology is important to the development of an integrated pest management program. Investigation was carried out on developmental biology of S. zeamais on four main cereal crops, maize, rice, sorghum, and millet, under laboratory conditions. Egg incubation, oviposition periods, and larval instar development were not different significantly among the food hosts. Number of eggs laid varied significantly among the cereal grains; mean fecundity was highest on maize (67.2±3.16 and lowest on millet (53.8±0.17. Number of immature (larva and pupa and adult stages varied significantly among the cereal grains. There exist four larval instars with a varied mean head capsule width, with a mean total instar larval developmental period of 23.1, 22.2, 22.2, and 21.6 d on maize, rice, sorghum, and millet, respectively. There was linear relationship and significant correlation between the stages of larval development and head capsule width. The mean developmental period from egg to adult varied, being highest on maize (34.7 d and lowest on sorghum (33.5 d.

  1. Erotylidae (Insecta, Coleoptera of Poland – problematic taxa, updated keys and new records

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    Rafal Ruta

    2011-10-01

    Full Text Available New data concerning the occurrence of pleasing fungus beetles (Coleoptera: Erotylidae in Poland are given, with a focus on rare and difficult to identify Central European taxa. Cryptophilus cf. integer (Heer (Cryptophilinae is reported from the Polish territory for the first time based on adult and larval specimens collected in the Wielkopolska-Kujawy Lowland. Identification problems concerning species of Cryptophilus introduced to Europe are discussed. Triplax carpathica Reitter (Erotylinae is recorded from the Białowieża Primeval Forest, which is the first known non-Carpathian finding of this species, located in the close proximity of the Polish-Belarussian UNESCO World Heritage Site “Białowieża Forest”. Discussion of T. carpathica being conspecific with Siberian T. rufiventris Gebler is provided. New Polish localities of several other Erotylidae are reported, and an updated key to Central European species of Triplax is given. The Triplax key is supplemented with dorsal and ventral habitus images of all treated Triplax species. One of the rarest Central European erotyline species Combocerus glaber (Schaller is recorded from xerothermic grasslands in North-West Poland.

  2. Fruit Damage Patterns Caused by Ovipositing Females of Conotrachelus dimidiatus (Coleoptera: Curculionidae in Guava Trees

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    Felipe Tafoya

    2010-01-01

    Full Text Available We evaluated the damage patterns produced by females of the guava weevil Conotrachelus dimidiatus Champion, 1904 (Coleoptera: Curculionidae, according to the position of the damaged fruit in guava trees Psidium guajava L. in Calvillo, Aguascalientes, Mexico. The trees were subdivided in eight zones, and during one year the level of fruit lesions due to oviposition was registered. Results showed a higher level of damage in the upper and external zone of the trees (P≤.05. We found no significant differences in damage between the four cardinal points (P≥.05. During the year, the level of damage was recorded and was higher in the months of August and September (P≤.05 associated with rainfall (0.86, P=.06 and increase in temperature (0.84, P=.03. The most susceptible fruits were in the size range of 2.1–4.0 cm (polar diameter. The information from this study will be used to design and establish effective control strategies for the guava weevil, taking into account location of the most susceptible fruits, seasonality of the pest, and the abiotic factors.

  3. Advances in the use of trapping systems for Rhynchophorus ferrugineus (Coleoptera: Curculionidae): traps and attractants.

    Science.gov (United States)

    Vacas, S; Primo, J; Navarro-Llopis, V

    2013-08-01

    Given the social importance related to the red palm weevil, Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae), efforts are being made to develop new control methods, such as the deployment of trapping systems. In this work, the efficacy of a new black pyramidal trap design (Picusan) has been verified in comparison with white and black buckets. In addition, the attractant and synergistic effect of ethyl acetate (EtAc) at different release levels has been evaluated under field conditions. The results show that Picusan traps captured 45% more weevils than bucket-type traps, offering significantly better trapping efficacy. The addition of water to traps baited with palm tissues was found to be essential, with catches increasing more than threefold compared with dry traps. EtAc alone does not offer attractant power under field conditions, and the release levels from 57 mg/d to 1 g/d have no synergistic effect with ferrugineol. Furthermore, significantly fewer females were captured when EtAc was released at 2 g/d. The implications of using EtAc dispensers in trapping systems are discussed.

  4. On the spatial spread of the Rice Water Weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Erirhinidae, in Italy

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    Daniela Lupi

    2010-07-01

    Full Text Available A five year study has been made to establish the spread of the rice water weevil Lissorhoptrus oryzophilus (Coleoptera: Erirhinidae in Northern Italy. Data obtained with GPS from 2005 throughout 2009 were first georeferenced with SW ArcGis® 9.2, then overlapped and compared to the map of the European environmental landscape based on the interpretation of satellite images (CORINE Land Cover map and to the hydrographic chart CT10 (Technical Regional map 10000. The analysis of the radial rate of spread per year indicates a deceleration in the expansion from 10.864 ± 6.801 km/year in 2005 to 5.318 ± 1.401 km/year in 2009. In five years the weevil has expanded its distribution in nearly all rice paddies in Lombardy and Piedmont, over an area of about 200,000 ha, which correspond to 86% of the total Italian rice area. Its expansion is thought to follow a type of stratified dispersal, due both to insect adult active dispersal and to accidental movements caused by human transportation.

  5. Transcriptomic Analysis of Eucryptorrhynchus chinensis (Coleoptera: Curculionidae) Using 454 Pyrosequencing Technology

    Science.gov (United States)

    Liu, Zhen-Kai; Wen, Jun-Bao

    2016-01-01

    Eucryptorrhynchus chinensis Olivier (Coleoptera: Curculionidae) is one of the most important pests of Ailanthus altissima; however, so far, no studies on the genome or transcriptome of E. chinensis have been reported. Using the Roche 454 FLX Titanium platform, an RNA pool obtained from E. chinensis eggs, larva, pupae, and adults was sequenced and assembled de novo to achieve maximum diversity of sampled transcripts. We obtained 1,441,137 (∼518 Mb) raw reads with an average length of 360 bp. After trimming, 89% qualified reads were produced and assembled into 35,509 isotigs with an average length of 440 bp, N50 of 1,048 bp, and 111,643 singletons. We generated 87,894 unigenes following a cluster analysis of the isotigs and singletons, and then functionally annotated the unigenes with gene descriptions. We obtained 23,363 GO assignments, and 12,724 unigenes were assigned to KOG. Based on these annotations, 294 biochemical pathways involved in growth, reproduction, and stress or immune responses were predicted. A total of 659,026 single nucleotide variants and 6,112 simple sequence repeats were detected. Our data provide comprehensive information on the sequence and possible functions of E. chinensis transcripts.

  6. Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil

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    Andreia Mauruto Chernaki-Leffer

    2011-03-01

    Full Text Available Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer, is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvos. Larvae were fed rabbit feed wetted with a triflumuron-water solution. Concentration-mortality regressions were estimated using Probit analysis and resistance ratios were calculated based on the susceptible population. Among the field populations evaluated, cypermethrin LC50 values for adults, ranged from 68.1 to 6,263 ng (AI/cm². LC50 values for adults challenged with dichlorvos ranged from 10.3 to 1,385 ng (AI/cm². One population from Pato Branco showed reduced susceptibility to triflumuron (LC50 = 272 µg (AI/ml of solution when compared to the most susceptible population (LC50 = 109.8 µg (AI/ml. Application of cypermethrin and dichlorvos analogues should be managed with caution to minimize insecticide resistance problems.

  7. Curative activity of insecticides against plum curculio (Coleoptera: Curculionidae) in tart cherries.

    Science.gov (United States)

    Hoffmann, Eric J; Vandervoort, Christine; Wise, John C

    2009-10-01

    Tart cherry, Prunus cerasus L. variety Montmorency, fruit were infested with plum curculio, Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), and treated with insecticides to target late instars, neonates, and eggs. The organophosphates azinphos-methyl and phosmet and the neonicotinoid thiamethoxam reduced larval emergence rates by >90% for all life stage targets; after >30 d, few surviving larvae were found inside fruit. Acetamiprid and thiacloprid also had curative activity and yielded >75% reductions in emergence and few surviving larvae in the fruit after >30 d. The juvenile hormone analog pyriproxyfen reduced larval emergence, but 66% of fruit that was treated to target late-instars still had live larvae inside of them after >30 d. Novaluron, chlorantraniliprole, and esfenvalerate had no curative activity. Indoxacarb had limited curative activity, and all targeted life stages had larval emergence. Internal and external residues were analyzed and are discussed in relation to their penetration and curative potential. The curative activity of azinphos-methyl has played an important role in meeting federal standards for infestation-free tart cherries at processing. Regulatory changes are eliminating the use of this compound, and new integrated pest management programs for plum curculio will need to address the loss of azinphos-methyl's curative activity. PMID:19886451

  8. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases.

    Science.gov (United States)

    Downey, Danielle; Chun, Stacey; Follett, Peter

    2015-06-01

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread.

  9. Species limits in polymorphic mimetic Eniclases net-winged beetles from New Guinean mountains (Coleoptera, Lycidae).

    Science.gov (United States)

    Bocek, Matej; Bocak, Ladislav

    2016-01-01

    Species delimitation was compared in a group of closely related lineages of aposematically colored Eniclases (Coleoptera, Lycidae) using morphology, genetic distances, and Bayesian implementation of the Poisson Tree Processes model. A high diversity of net-winged beetles was found in previously unsampled regions of New Guinea and ten new species are described: Eniclases bicolor sp. n., Eniclases bokondinensis sp. n., Eniclases brancuccii sp. n., Eniclases elelimensis sp. n., Eniclases infuscatus sp. n., Eniclases niger sp. n., Eniclases pseudoapertus sp. n., Eniclases pseudoluteolus sp. n., Eniclases tikapurensis sp. n., and Eniclases variabilis sp. n. Different levels of genetic and morphological diversification were identified in various sister-species pairs. As a result, both morphological and molecular analyses are used to delimit species. Sister-species with uncorrected pairwise genetic divergence as low as 0.45% were morphologically distinct not only in color pattern, but also in the relative size of eyes. Conversely, differences in color pattern regardless of their magnitude did not necessarily indicate genetic distance and intraspecific mimicry polymorphism was common. Additionally, genetic divergence without morphological differentiation was detected in one sister-species pair. Low dispersal propensity, diverse mimicry patterns, and mimetic polymorphism resulted in complex diversification of Eniclases and uncertain species delimitation in recently diversified lineages.

  10. Notas e novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae

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    Ubirajara R. Martins

    2007-01-01

    Full Text Available Novas espécies descritas do Brasil: Hypsioma carioca sp. nov. (Rio de Janeiro e Hesychotypa maraba sp. nov. (Pará; do Equador: Sternycha ecuatoriana sp. nov. (Pichincha; da Bolívia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. e Alexera secunda sp. nov. Transfere-se Hesycha strandi (Breuning, 1943 para o gênero Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 é considerada sinônima de H. miniata Thomson, 1868. Novo registro (Trinidad e figura são dados para Trachysomus surdus.Notes and new species of Onciderini (Coleoptera, Cerambycidae, Lamiinae. New species described from Brazil: Hypsioma carioca sp. nov. (Rio de Janeiro and Hesychotypa maraba sp. nov. (Pará; from Ecuador: Sternycha ecuatoriana sp. nov. (Pichincha; from Bolivia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. and Alexera secunda sp. nov. Hesycha strandi (Breuning, 1943 is transferred to the genus Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 is considered a synonym of H. miniata Thomson, 1868. New record (Trinidad and figure for Trachysomus surdus Dillon & Dillon, 1946 are given.

  11. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect

    International Nuclear Information System (INIS)

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5–7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1–4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. (author)

  12. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae

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    M.M. Kiradoo

    2011-03-01

    Full Text Available The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insecticides, there is an utmost need to search for some alternate methods for protection against the damage caused by insects. The leaves of the select plants viz., Ocimum basilicum, O. sanctum and Mentha spicata were employed in the form of various formulations and mortality of the pest insect was assessed. A significant increase in mortality of C. chinensis was observed in all the experimental sets as compared to normal and control sets during the present study. When comparisons were made on the basis of ANOVA to compare the effect of the three plants on mortality, it was found that the treatments of O. basilicum were superior over M. spicata and O. sanctum. The findings overall suggest that all the three plants screened have a potential to be used against the pest C. chinensis and can be employed as an alternate to chemical insecticides in household and storehouses to minimize the infestation and damage caused by the bruchid.

  13. Ecological, morphological, and histological studies on Blaps polycresta (Coleoptera: Tenebrionidae) as biomonitors of cadmium soil pollution.

    Science.gov (United States)

    Osman, Wafaa; El-Samad, Lamia M; Mokhamer, El-Hassan; El-Touhamy, Aya; Shonouda, Mourad

    2015-09-01

    Soil pollution in Egypt became far more serious than before due to either the heavy usage of different toxic pesticides or aerosol deposition of industrial pollutants. The present mentioned ground beetle, Blaps polycresta Tschinkel 1975 (Coleoptera: Tenebrionidae), showed ecological, morphological, and histological alterations in adult insects as biomonitors. Two cultivated sites (reference and polluted) were chosen for sampling the insects. The results indicated a significant increase in soil cadmium concentration of the polluted site leading to sex-specific difference in cadmium accumulation in gonads and alimentary canal of insects that being higher in males than females. The cadmium pollution leads significantly to a decrease in population density, a reduction in body weight, an increase in mortality rate, and an increase in sex ratio of the insects. The results also revealed a striking decrease in body length of the polluted insects with a marked increase in the percentage of deformed gonads and alimentary canal of both sexes. Some histopathological alterations were also recorded in testis, ovary, and midgut of the polluted insects. Our results confirmed that beetles are a good bioindicator for soil pollution, and the different studied parameters could be easily employed as sensitive monitors for cadmium soil pollution.

  14. Caracteres preimaginales y aspectos bionómicos de Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae

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    Jerez, V.

    2011-12-01

    Full Text Available We describe the morphology and microstructure of the egg chorion, and the morphology of the first instar larva of Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae, Nycteliini. Bionomical and distributional data on this species are also provided. To obtain eggs and larvae, couples were collected in the field and kept in rearing cages until oviposture and ecclosion. The structure and adornment of the egg exochorion, and the exterior morphological features of larvae were examined with electron scanning microscopy. The eggs of G. luczotii showed a rounded micropyle and a smooth exochorion, composed of hexagonal cells without aeropyles. The larvae of G. luczotii showed morphological characteristics suited for an edaphic life similar to that of Pedobionta: digging prothoracic legs, cephalic capsule with abundant sensilla, and well–developed pygopodium. We analysed the importance of larval morphology as an element for specific diagnosis and found that interspecific differences regarding frontal sensilla, clypeus shape, and anterior part of labrum, had a taxonomic value and possibly a phylogenetic value.

  15. BIOECOLOGY AND MANAGEMENT OF ERVA-MATE BORER, Hedypathes betulinus (KLUG, 1825 (COLEOPTERA: CERAMBYCIDAE

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    Márcia d´Avila

    2010-08-01

    Full Text Available The erva-mate (Ilex paraguariensis A.St.-Hil. has a social, cultural and economic importance in  the southern states of Brazil. The  pure stands of  this culture was responsible for the increase  of many species of insects. Hedypathes betulinus (Klug, 1825 (Coleoptera: Cerambycidae is considered the main pest from an economic viewpoint because of its difficult control and potential for damage. The larval phase occurs inside the twings and trunks, what makes more difficult to deal with its detention and management. Cultural and mechanical management are the most indicated, such as collection of adults, prunning and burning of plant parts damaged by the insect, balanced nutrition, adequate plant density and maintenance of areas with native vegetation or also the introduction of policulture. These strategies  may increase  the  agroecossystem  balance  and  thus  a  reduction  of  the  insect-pest  to  an aceptable level. Therefore, the objective of this study was to assemble and the discuss the information on the bioecology and management of erva-mate borer.

  16. Karyotype differentiation patterns in species of the subfamily Scarabaeinae (Scarabaeidae, Coleoptera).

    Science.gov (United States)

    Cabral-de-Mello, Diogo Cavalcanti; de Oliveira, Sárah Gomes; Ramos, Ituza Celeste; de Moura, Rita de Cássia

    2008-12-01

    The aim of this study was to describe the karyotype of species belonging to the subfamily Scarabaeinae (Coleoptera, Scarabaeidae) and to compile the conventional cytogenetic data available in the literature for this group. The karyotypes of ten species belonging to the tribes Canthonini, Coprini, Onthophagini and Phanaeini were analyzed by conventional staining. Eight of these species were described for the first time (Canthon aff carbonarius, Canthon chalybaeus, Coprophanaeus dardanus, Deltochilum aff amazonicum, Dichotomius geminatus, Oxysternon silenus, Phanaeus chalcomelas and Malagoniella aff astyanax) and two were redescribed (Diabroctis mimas and Digitonthophagus gazella) since their karyotypes differed from those previously published in the literature. Four species studied showed a diploid number of 2n=20 and a parachute type sex determining system and the karyotype was 2n=20,Xy in two species and 2n=18,Xy(p), 2n=19,X0, 2n=12,XY and 2n=14,neoXY in one each. The chromosome morphology of the different species varied, with the observation of metacentric, submetacentric, subacrocentric and acrocentric chromosomes. The X chromosome was predominantly meta or submetacentric in the species analyzed, whereas the y chromosome presented two arms or was punctiform. In conclusion, the subfamily Scarabaeinae comprises 120 species analyzed cytogenetically, and are observed the occurrence of five chromosome rearrangements (autosome-autosome and X-autosome fusions, pericentric inversions, fissions and loss of the y chromosome) that are related to the chromosome variability and evolution in the group. PMID:18495484

  17. A multilayer micromechanical model of the cuticle of Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae).

    Science.gov (United States)

    Andrew Jansen, M; Singh, Sudhanshu S; Chawla, Nikhilesh; Franz, Nico M

    2016-08-01

    Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae), is a weevil species common throughout the southwestern United States that uses its rostrum - a very slender, curved, beak-like projection of the head - to excavate tunnels in plant organs (such as acorns) for egg laying (oviposition). Once the apical portion of the rostrum has been inserted into the preferred substrate for oviposition, the female begins rotating around the perimeter of the hole, elevating her head by extending the fore-legs, and rotating the head in place in a drilling motion. This action causes significant elastic deformation of the rostrum, which will bend until it becomes completely straight. To better understand the mechanical behavior of the cuticle as it undergoes deformation during the preparation of oviposition sites, we develop a comprehensive micro/macro model of the micromechanical structure and properties of the cuticle, spanning across all cuticular regions, and reliably mirroring the resultant macroscale properties of the cuticle. Our modeling approach relies on the use of multi-scale, hierarchical biomaterial representation, and employs various micromechanical schemata - e.g., Mori-Tanaka, effective field, and Maxwell - to calculate the homogenized properties of representative volume elements at each level in the hierarchy. We describe the configuration and behavior of this model in detail, and discuss the theoretical implications and limitations of this approach with emphasis on future biomechanical and comparative evolutionary research. Our detailed account of this approach can thereby serve as a methodological template for exploring the biomechanical behavior of new insect structures. PMID:27189867

  18. Coexistence and Competition between Tomicus yunnanensis and T. minor (Coleoptera: Scolytinae in Yunnan Pine

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    Rong Chun Lu

    2012-01-01

    Full Text Available Competition and cooperation between bark beetles, Tomicus yunnanensis Kirkendall and Faccoli and Tomicus minor (Hartig (Coleoptera: Scolytinae were examined when they coexisted together in living Yunnan pine trees (Pinus yunnanensis Franchet in Yunnan province in Southwest China. T. yunnanensis bark beetles were observed to initiate dispersal from pine shoots to trunks in November, while the majority of T. minor begins to transfer in December. T. yunnanensis mainly attacks the top and middle parts of the trunk, whereas T. minor mainly resides in the lower and middle parts of the trunk. The patterns of attack densities of these two species were similar, but with T. yunnanensis colonizing the upper section of the trunk and T. minor the lower trunk. The highest attack density of T. Yunnanensis was 297 egg galleries/m2, and the highest attack density of T. minor was 305 egg galleries/m2. Although there was significant overlap for the same bark areas, the two species generally colonize different areas of the tree, which reduces the intensity of competition for the relatively thin layer of phloem-cambium tissues where the beetles feed and reside.

  19. Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera)

    Science.gov (United States)

    Pineda-Castellanos, Mónica L.; Rodríguez-Segura, Zitlhally; Villalobos, Francisco J.; Hernández, Luciano; Lina, Laura; Nuñez-Valdez, M. Eugenia

    2015-01-01

    Serratia marcescens is a Gram negative bacterium (Enterobacteriaceae) often associated with infection of insects. In order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased Phyllophaga spp (Coleoptera:Scarabaeidae) larvae collected from cornfields in Mexico. Five isolates were identified as Serratia marcescens by 16S rRNA gene sequencing and biochemical tests. Oral and injection bioassays using healthy Phyllophaga blanchardi larvae fed with the S. marcescens isolates showed different degrees of antifeeding effect and mortality. No insecticidal activity was observed for Spodoptera frugiperda larvae (Lepidoptera: Noctuidae) by oral inoculation. S. marcescens (Sm81) cell-free culture supernatant caused significant antifeeding effect and mortality to P. blanchardi larvae by oral bioassay and also mortality by injection bioassay. Heat treated culture broths lost the ability to cause disease symptoms, suggesting the involvement of proteins in the toxic activity. A protein of 50.2 kDa was purified from the cell-free broth and showed insecticidal activity by injection bioassay towards P. blanchardi. Analysis of the insecticidal protein by tandem- mass spectrometry (LC-MS/MS) showed similarity to a Serralysin-like protein from S. marcescens spp. This insecticidal protein could have applications in agricultural biotechnology. PMID:25984910

  20. Ground beetles (Coleoptera, Carabidae of the Hanford Nuclear Site in south-central Washington State

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    Chris Looney

    2014-04-01

    Full Text Available In this paper we report on ground beetles (Coleoptera: Carabidae collected from the Hanford Nuclear Reservation and Hanford National Monument (together the Hanford Site, which is located in south-central Washington State. The Site is a relatively undisturbed relict of the shrub-steppe habitat present throughout much of the western Columbia Basin before the westward expansion of the United States. Species, localities, months of capture, and capture method are reported for field work conducted between 1994 and 2002. Most species were collected using pitfall traps, although other capture methods were employed. Trapping results indicate the Hanford Site supports a diverse ground beetle community, with over 90% of the 92 species captured native to North America. Four species collected during the study period are newly recorded for Washington State: Bembidion diligens Casey, Calosoma obsoletum Say, Pseudaptinus rufulus (LeConte, and Stenolophus lineola (Fabricius. Based on these data, the Site maintains a diverse ground beetle fauna and, due to its size and diversity of habitats, is an important repository of shrub-steppe biodiversity.

  1. Ground beetles (Coleoptera, Carabidae) of the Hanford Nuclear Site in south-central Washington State

    Science.gov (United States)

    Looney, Chris; Zack, Richard S.; LaBonte, James R.

    2014-01-01

    Abstract In this paper we report on ground beetles (Coleoptera: Carabidae) collected from the Hanford Nuclear Reservation and Hanford National Monument (together the Hanford Site), which is located in south-central Washington State. The Site is a relatively undisturbed relict of the shrub-steppe habitat present throughout much of the western Columbia Basin before the westward expansion of the United States. Species, localities, months of capture, and capture method are reported for field work conducted between 1994 and 2002. Most species were collected using pitfall traps, although other capture methods were employed. Trapping results indicate the Hanford Site supports a diverse ground beetle community, with over 90% of the 92 species captured native to North America. Four species collected during the study period are newly recorded for Washington State: Bembidion diligens Casey, Calosoma obsoletum Say, Pseudaptinus rufulus (LeConte), and Stenolophus lineola (Fabricius). Based on these data, the Site maintains a diverse ground beetle fauna and, due to its size and diversity of habitats, is an important repository of shrub-steppe biodiversity. PMID:24715791

  2. FAUNÍSTIC STUDY OF BEETLES (COLEOPTERA IN A SILVICULTURAL-PASTORAL SYSTEM

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    Alexander Machado Auad

    2011-03-01

    Full Text Available The aim of this study was to conduct a survey of beetles (Coleoptera in a silvicultural-pastoral system, estimating constancy, abundance, richness, diversity of families and seasonality, from July 2006 to June 2008. The study was carried out at the Embrapa Dairy Cattle station, in Coronel Pacheco, Minas Gerais, Brazil. The beetles were collected in Malaise traps, every two weeks, and taken to the laboratory for analysis. A total of 26 families, 294 morphospecies and 1,606 specimens were found. The Elateridae, Mordelidae, Chrysomelidae, Coccinellidae and Curculionidae families were the most abundant, rich and diverse. The Scarabaeidae and Scolytidae families were also among the most abundant, and the Cerambycidae family was among the richest, while the Bruchidae and Carabidae families presented high diversity.  The largest number of individuals and morphospecies sampled occurred during the period of rain. November 2007 presented the most (n = 535 specimens captures of any month, followed by October 2006, September 2007, March and April of 2008.

  3. South American Coccinellidae (Coleoptera: part X: A systematic revision of Thalassa Mulsant (Hyperaspidinae

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    Julianne Milléo

    2004-01-01

    Full Text Available The genus Thalassa Mulsant, 1850 is revised. Its five species are redescribed: T. pentaspilota (Chevrolat, 1853, T. flaviceps Mulsant, 1850, T. similaris Mulsant, 1850, T. montezumae Mulsant, 1850, and T. glauca (Mulsant, 1850. A new species is proposed, Thalassa korschefskyi sp.nov., from Colombia. The species are illustrated and a key to species is also provided. Lectotype of Thalassa reyi Mulsant, 1850 is here designated.Coccinellidae (Coleoptera da América do Sul. Parte X: Revisão sistemática de Thalassa Mulsant (Hyperaspidinae. O gênero Thalassa Mulsant, 1850 é revisado. Suas cinco espécies são redescritas: T. pentaspilota (Chevrolat, 1853, T. flaviceps Mulsant, 1850, T. similaris Mulsant, 1850, T. montezumae Mulsant, 1850 e T. glauca (Mulsant, 1850. Uma nova espécie é proposta, Thalassa korschefskyi sp. nov., da Colômbia. São fornecidas ilustrações e chave para as espécies. O lectótipo de Thalassa reyi Mulsant, 1850 é aqui designado.

  4. Naturally-Occurring Entomopathogenic Fungi on Three Bark Beetle Species (Coleoptera: Curculionidae in Bulgaria

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    Slavimira A. Draganova

    2010-01-01

    Full Text Available Bark beetles (Coleoptera: Curculionidae: Scolytinae belong to one of the most damaging groups of forest insects and the activity of their natural enemies –pathogens, parasitoids,parasites or predators suppressing their population density,is of great importance. Biodiversity of entomopathogenic fungi on bark beetles in Bulgaria has been investigated sporadically. The aim of this preliminary study was to find, identify and study morphological characteristics of fungal entomopathogens naturally-occurring in populations of three curculionid species – Ips sexdentatus Boern, Ips typographus (L. and Dryocoetes autographus (Ratz.. Dead pest adults were found under the bark of Pinus sylvestris and Picea abies trees collectedfrom forests in the Maleshevska and Vitosha Mountains. Fungal pathogens were isolated into pure cultures on SDAY (Sabouraud dextrose agar with yeast extract and were identified based on morphological characteristics both on the host and in a culture.Morphological characteristics of the isolates were studied by phenotypic methods. The fungal isolates obtained from dead adults of Ips sexdentatus, Ips typographus and D. autographus were found to belong to the species Beauveria bassiana (Bals. – Criv. Vuillemin,Beauveria brongniartii (Saccardo Petch and Isaria farinosa (Holmsk. Fries (anamorph Ascomycota, Sordariomycetes: Hypocreales, Cordycipitaceae. Morphological traits of the isolates are described.

  5. Pitfall Traps and Mini-Winkler Extractor as Complementary Methods to Sample Soil Coleoptera.

    Science.gov (United States)

    Carneiro, A C; Batistella, D A; Battirola, L D; Marques, M I

    2016-02-01

    We compared abundance, species richness, and capture efficiency with pitfall traps and mini-Winkler extractors to examine their use as complementary methods for sampling soil Coleoptera during dry (2010) and high water seasons (2011) in three areas, including inundated and non-inundated regions, in the Pantanal of Poconé, Mato Grosso, Brazil. We paired treatments with two 10 × 10 m plots in inundated and non-inundated locations that were repeated three times in each location for a total of 18 plots. In each plot, we used nine pitfall traps and collected 2 m(2) of leaf litter and surface soil samples with mini-Winkler extractors. We collected a total of 4260 adult beetles comprising 36 families, 113 genera, and 505 species. Most were caught in pitfalls (69%) and the remainder in the mini-Winkler extractors (31%). Each method provided distinct information about the beetle community: 252 species were captured only in pitfall traps, 147 using only the mini-Winkler extractors, and these methods shared another 106 species. Pitfall and mini-Winkler contribute in different ways for the sampling of the soil beetle community, and so they should be considered complementary for a more thorough assessment of community diversity. PMID:26493175

  6. Oil pipeline corridor through an intact forest alters ground beetle (Coleoptera: Carabidae) assemblages in southeastern Ohio.

    Science.gov (United States)

    Silverman, Bareena; Horn, David J; Purrington, Foster F; Gandhi, Kamal J K

    2008-06-01

    Litter-dwelling ground beetle (Coleoptera: Carabidae) assemblages were monitored 1 yr after the construction of a corridor for installation of an oil pipeline along a xeric ridge-top forest in southeastern Ohio. After the creation of the corridor, three distinct habitats were evident in these sites: open corridor, ecotone areas around the corridor, and undisturbed forest interior. Carabidae were collected using directional pitfall traps that were placed parallel and perpendicular to the corridor in each of the three habitats. Results indicate that more carabids were present in the ecotone than in the other two habitats. Carabid diversity as estimated by rarefaction was highest in the corridor followed by ecotone and forest interior. Generalist and forest specialists such as Synuchus impunctatus (Say), Carabus goryi Dejean, and Pterostichus trinarius (Casey) were present in greater numbers in the forest interior and ecotone assemblages. In contrast, open-habitat specialists such as Harpalus pensylvanicus (DeGeer) and Selenophorus opalinus (LeConte) were present in greater numbers in the corridor assemblages. Carabid assemblages of the corridor were distinct from those of the ecotone and forest interior, whereas the latter two habitats had very similar assemblages. The successional pathway of the corridor carabid assemblage will therefore be likely different from that of the forest interior and ecotone. Overall, results indicate that construction of the oil pipeline corridor had significant short-term effects on the carabid numbers, diversity, and species composition because of ensuing habitat changes and fragmentation of the forest. PMID:18559178

  7. Demographic comparison and population projection of Rhynchophorus ferrugineus (Coleoptera: Curculionidae) reared on sugarcane at different temperatures.

    Science.gov (United States)

    Peng, Lu; Miao, Yunxin; Hou, Youming

    2016-01-01

    Understanding how temperature affects fitness is important for conservation and pest management, especially in the era of global climate change. Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae) is a worldwide pest of many economically important crops. Although much is known about this pest's life cycle, its adaptability to different temperatures is not fully understood. Here, we used age- and stage-specific life tables to investigate the effects of temperature on fitness-related traits and demographic parameters of R. ferrugineus under eight constant temperature regimens in the laboratory. The growth potential of these populations was also evaluated. The greatest longevity for males and females was 158.0 d at 24 °C and 144.5 d at 21 °C, respectively, but mean total fecundity was the highest at 27 °C. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R0) increased initially at low temperatures and then decreased. All metrics reached a maximum at 27 °C and a minimum at 36 °C. Mean generation times (T ) decreased across the temperature range with a minimum at 36 °C. Our results indicate that the optimum temperature for growth of R. ferrugineus was approximately 27 °C. Our work will be of value for developing strategies for control management of this pest species. PMID:27545594

  8. Volatiles released from Vaccinium corymbosum were attractive to Aegorhinus superciliosus (Coleoptera: Curculionidae) in an olfactometric bioassay.

    Science.gov (United States)

    Parra, Leonardo; Mutis, Ana; Ceballos, Ricardo; Lizama, Marcelo; Pardo, Fernando; Perich, Fernando; Quiroz, Andrés

    2009-06-01

    The objective of this study was to evaluate the role of host volatiles in the relationship between a blueberry plant Vaccinium corymbosum L. and the raspberry weevil Aegorhinus superciliosus (Guérin) (Coleoptera: Curculionidae), the principal pest of blueberry in the south of Chile. Volatiles from the aerial part of different phenological stages of the host were collected on Porapak Q and analyzed by coupled gas chromatography-mass spectrometry (GC-MS). Several chemical groups were identified including green leaf volatiles, aromatic compounds, and terpenes. The olfactometric responses of A. superciliosus toward different odor sources were studied in a four-arm olfactometer. Blueberry shoots at the phenological stages of fruit set, and blue-pink fruit color elicited the greatest behavioral responses from weevils. Five compounds (2-nonanone, eucalyptol, R- and S-limonene, and 4-ethyl benzaldehyde) elicited an attractant behavioral response from A. superciliosus. The results suggest the host location behavior of A. superciliosus could be mediated by volatiles derived from V. corymbosum. This work has identified a number of compounds with which it is possible to develop a lure for the principal pest of blueberry in southern Chile.

  9. Males of Hylamorpha elegans burmeister (Coleoptera: Scarabaeidae) are attracted to odors released from conspecific females.

    Science.gov (United States)

    Quiroz, Andrés; Palma, Ruben; Etcheverría, Paulina; Navarro, Vicente; Rebolledo, Ramón

    2007-04-01

    The behavioral responses of Hylamorpha elegans L. (Coleoptera: Scarabaeidae, Rutelinae) to the semiochemicals released from conspecific individual adults were studied, with particular attention paid to female attraction of males. Odors released from virgin females significantly attracted male conspecifics in both the field and laboratory olfactometer and wind tunnel bioassays. However, females did not attract other females, and males attracted no one. The response of male H. elegans to (1) compounds (1,4-hydroquinone and 1,4-benzoquinone) released only by unmated females; (2) the essential oil of the secondary host (Nothofagus obliqua); and (3) the blend of 1,4-hydroquinone and 1,4-benzoquinone with N. obliqua essential oil was studied. The blend of 1,4-benzoquinone mixed with essential oil at the trial concentration was attractive with males. The same response was found with 1,4-hydroquinone alone. The essential oil did not have the expected attractant effect on conspecific males. These results suggest that, when combined with essential oil, 1,4-benzoquinone may function in the sexual behavior of males and females. These findings are discussed in terms of the ecological role of this putative sexual pheromone and its potential use in a strategy of control of this pest.

  10. The Hydraulic Mechanism of the Unfolding of Hind Wings in Dorcus titanus platymelus (Order: Coleoptera

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    Jiyu Sun

    2014-04-01

    Full Text Available In most beetles, the hind wings are thin and fragile; when at rest, they are held over the back of the beetle. When the hind wing unfolds, it provides the necessary aerodynamic forces for flight. In this paper, we investigate the hydraulic mechanism of the unfolding process of the hind wings in Dorcus titanus platymelus (Oder: Coleoptera. The wing unfolding process of Dorcus titanus platymelus was examined using high speed camera sequences (400 frames/s, and the hydraulic pressure in the veins was measured with a biological pressure sensor and dynamic signal acquisition and analysis (DSA during the expansion process. We found that the total time for the release of hydraulic pressure during wing folding is longer than the time required for unfolding. The pressure is proportional to the length of the wings and the body mass of the beetle. A retinal camera was used to investigate the fluid direction. We found that the peak pressures correspond to two main cross-folding joint expansions in the hind wing. These observations strongly suggest that blood pressure facilitates the extension of hind wings during unfolding.

  11. Registros y distribución de la especie afroasiática Digitonthophagus gazella (Coleoptera: Scarabaeidae: Scarabaeinae en Argentina Records and distribution of the Afro Asian species Digitonthophagus gazella (Coleoptera: Scarabaeidae: Scarabaeinae in Argentina

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    María C. Álvarez Bohle

    2009-12-01

    Full Text Available Se cita por primera vez el escarabajo coprófago Digitonthophagus gazella Fabricius (Coleoptera: Scarabaeidae para Argentina, por lo que se amplía su área de distribución geográfica en Sudamérica. Esta especie de origen afro-asiático fue capturada mediante colecta manual en estiércol de ganado bovino y equino, con trampas de luz y trampas de caída cebadas con estiércol en seis provincias del centro y norte argentino entre los años 2006 y 2009.The occurrence of the dung beetle Digitonthophagus gazella Fabricius (Coleoptera: Scarabaeidae is reported for the first time for Argentina. The record of this species represents an extension of its geographical range in South America. This species of Afro Asian origin was captured by manual collection in cattle dung, light traps, and dung baited pitfall traps in six provinces of Northern and Central Argentina between 2006 and 2009.

  12. Danos de Conotrachelus dubiae (Coleoptera: curculionidae em frutos de camu-camu (Myrciaria dubia na Amazônia Central Damage of camu-camu (Myrciaria dubia fruits by Conotrachelus dubiae (Coleoptera: curculionidae in Central Amazonia

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    Sidney Alberto do Nascimento Ferreira

    2003-12-01

    Full Text Available No Brasil, a ocorrência de Conotrachelus dubiae O'Brien, 1995 (Coleoptera: Curculionidae em camu-camu [Myrciaria dubia (H.B.K. McVaugh, Myrtaceae] tinha sido constatada somente em populações naturais. Relata-se sua ocorrência em um cultivo experimental, onde se avaliou os danos de C. dubiae em frutos de camu-camu, em diferentes graus de amadurecimento, entre 1999 e 2003. Os danos causados pela larva aumentaram com o amadurecimento dos frutos, havendo maior comprometimento da polpa do fruto (30 a 90% do que das sementes (7%. A incidência desse inseto pode implicar em perdas quantitativas significativas na produção de camu-camu.In Brazil, the occurrence of Conotrachelus dubiae O'Brien, 1995 (Coleoptera: Curculionidae in camu-camu [Myrciaria dubia (H.B.K. McVaugh, Myrtaceae] had only been verified in natural populations. This report describes its occurrence in an experimental cultivation, where damage of camu-camu fruits by C. dubiae at different ripening stages was evaluated between 1999 and 2003. The damage caused by the larva increased with the degree of ripening of the fruits, with greater damage of fruit pulp (30 to 90% than to seeds (7%. The incidence of this insect may cause significant quantitative losses in the camu-camu production.

  13. Los coleópteros y heterópteros acuáticos del Parque Nacional Calilegua (Provincia de Jujuy, Argentina Aquatic Coleoptera and Heteroptera from Calilegua National Park (Jujuy Province, Argentina

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    Patricia L. M. Torres

    2008-06-01

    Full Text Available Se expone una lista de las especies de Coleoptera y Heteroptera presentes en los cuerpos de agua del Parque Nacional Calilegua. Se registraron 51 especies de Coleoptera incluidas en 28 géneros y cinco familias, y 37 especies de Heteroptera distribuidas en 22 géneros y 10 familias; sólo cuatro especies de Coleoptera y cinco de Heteroptera fueron citadas previamente del parque. Se mencionan por primera vez para la Argentina dos especies de Veliidae (Heteroptera: Rhagovelia trista Gould y Platyvelia brachialis (Stål, así como una especie y un género de Dytiscidae (Coleoptera: Desmopachria chei K. B. Miller y Bidessonotus Régimbart. Además, es la primera ocasión en que se mencionan para la provincia de Jujuy 24 especies y 11 géneros de Coleoptera (Leuronectes Sharp, Bidessonotus, Hypodessus Guignot, Desmopachria Babington, Celina Aubé, Gyrinus Linné, Haliplus Latreille, Hydrocanthus Say, Suphis Aubé, Anacaena Thomson, Derallus Sharp y 19 especies y 12 géneros de Heteroptera (Mesovelia Mulsant & Rey, Hebrus Curtis, Merragata White, Microvelia Westwood, Rhagovelia Mayr, Platyvelia J. T. Polhemus & D. A. Polhemus, Limnogonus Stål, Curicta Stål, Ranatra Fabricius, Centrocorisa Lundblad, Pelocoris Stål, Neoplea Esaki & China.A list of the species of aquatic Coleoptera and Heteroptera occurring in Calilegua National Park is presented. Fifty-one species of Coleoptera included in 28 genera and five families, and 37 species of Heteroptera belonging to 22 genera and 10 families were recorded. Only four species of Coleoptera and five of Heteroptera had been previously cited from the park. Two species of Veliidae (Heteroptera (Rhagovelia trista Gould, Platyvelia brachialis (Stål, along with one species and one genus of Dytiscidae (Coleoptera (Desmopachria chei K. B. Miller, Bidessonotus Régimbart are new records for Argentina. Twenty-four species and 11 genera of Coleoptera (Leuronectes Sharp, Bidessonotus, Hypodessus Guignot, Desmopachria

  14. Cowpea Vicilins: Fractionation of Urea Denatured Sub-Units and Effects on Callosobruchus maculatus F. (Coleoptera: Bruchidae Development

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    Antônio Chagas Mota

    2002-03-01

    Full Text Available Vicilins (7S storage globulins isolated from cowpea (Vigna unguiculata L. seeds which were susceptible (S and resistant (R to the cowpea weevil (Callosobruchus maculatus F., Coleoptera: Bruchidae were denatured by urea and fractionated by ion-exchange chromatography. Isolated fractions were incorporated in artificial seeds for assessment of their toxicity to C. maculatus. The most acidic fractions of both susceptible (CE-31 cultivar and resistant (IT81D-1045 line seeds were shown to affect development and survival of the bruchid. Results indicated that vicilin polypeptides of toxic nature were expressed in both types of storage globulins although at different levels.Vicilinas (globulinas de reserva 7S isoladas de sementes de feijão-de-corda (Vigna unguiculata L., susceptíveis (S e resistentes (R ao caruncho/gorgulho (Callosobruchus maculatus F., Coleoptera: Bruchidae foram desnaturadas por uréia e fracionadas por cromatografia de troca iônica. As frações isoladas foram incorporadas em sementes artificiais para avaliação de sua toxicidade a C. maculatus. As fracões mais ácidas de ambas vicilinas afetaram o desenvolvimento e a sobrevivência do bruquídeo. Sugerimos que polipeptídeos de vicilinas de natureza tóxica são expressos em ambos tipos de globulinas de reserva, embora em níveis diferentes.

  15. Capture of Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) in Floor Traps: The Effect of Previous Captures.

    Science.gov (United States)

    Athanassiou, Christos G; Kavallieratos, Nickolas G; Campbell, James F

    2016-02-01

    The impact of prior captures on the trapping performance of floor traps was evaluated for the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), and the confused flour beetle, Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae), in laboratory conditions. The effect of trap seeding, adding adults of the same or different species, was evaluated in order to determine possible effects of prior captures in the trap on each species' behavioral responses. The presence of seeded beetles of the same species resulted in an increase in beetle captures for both T. castaneum and T. confusum, but when traps were seeded with the opposite species, there was no increase in beetle captures for either species, and for T. castaneum overall captures in both seeded and unseeded traps was reduced. Overall, T. castaneum tended to have greater captures than T. confusum regardless of the treatment. When the two species were released together, this negated the increased response to seeded traps observed in the single-species treatments. These findings suggest the potential that the presence of beetles in a trap may be influencing the response of beetles in a nearby trap and that T. castaneum and T. confusum when they occur together may influence each other's response to traps.

  16. Longhorn beetles of the Ficuzza woods (W Sicily, Italy and their relationship with plant diversity (Coleoptera, Cerambycidae

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    Tommaso La Mantia

    2010-12-01

    Full Text Available The woods in Sicily are the result of centuries of anthropogenic activities that have reduced the surface of woodand changed the original composition even with the introduction of alien species to native flora. The value interms of biodiversity of these forests remains, however, high for they are the last refuge areas for many animalsand plant species. This study was conducted within the Ficuzza woods (West Sicily, extended about 5,000hectares on the slopes of limestone-dolomite rock of Busambra (1615 m asl, within which lies the largestremaining forest area in western Sicily. It is an area with a wide diversity of vegetation, represented mainly bynative forests (holm oak, cork oak, deciduous oaks, groups of riparian vegetation, shrubs, bushes, grasslands,and of non-native forest formations (Pinus and Eucalyptus woods. The study on Cerambycidae in this area isfragmented and does not specify a relation the species with the surrounding vegetation. This study wasperformed by choosing among various groups of insects, xylophagous Coleoptera Cerambycidae; existingliterature data and extensive collected field data were reviewed. The analysis was also performed by thecollection of dead wood in order to distinguish the relationship between the plant species and coleoptera. Theresults summarize and supplement the data registered so far, shedding further light on the ecological role ofthis group of insects that are also valid biomarkers of the integrity and complexity of the forest.

  17. 从花斑糠疹皮损分离的马拉色菌随机扩增多态性DNA研究%Random amplified polymorphic DNA analysis of Malassezia isolates from cutaneous lesions of pityriasis versicolor

    Institute of Scientific and Technical Information of China (English)

    谢震; 冉玉平; 刘瑞; 杨如学; 李志瑜; 代亚玲

    2009-01-01

    目的 用随机扩增多态性DNA方法 研究分离自花斑糠疹的马拉色菌种间和株间差异,了解随机扩增多态性DNA分析(RAPD)与生理生化方法 在菌种分型上的差异及菌株DNA型别和菌种间的关系.方法 用氯化苄法提取马拉色菌标准株(10株7个种)和临床分离株(47株)的基因组DNA,其中临床株分离自34例花斑糠疹患者,经形态学和生理生化方法 鉴定为5个种(合轴马拉色菌、糠秕马拉色菌、钝形马拉色菌、球形马拉色菌、限制马拉色菌),用4种随机引物(S22、SS24、S25、S33)对菌株DNA做PCR随机扩增,NTSYS软件自动生成树状分支图.结果 绝大多数标本均可被4种引物扩增而获得清晰条带,其中2种引物(S22、S24)的条带更为稳定、清晰.共82条DNA片段被扩增,所有菌株均可见种间和株间多态性.有4例患者皮损同时分离出不同种的菌株显示遗传相似性高,在树状图中归入一类.结论 来自同一宿主的不同菌株遗传趋同现象提示马拉色菌的种特异性、菌种演化与宿主间存在密切关系.%Objective To investigate intraspecific and interspecific variation within Malassezia iso-lates from patients with pityriasis versicolor by random amplified polymorphic DNA (RAPD) analysis, to learn the difference between RAPD analysis and physiological and biochemical methods in the typing of Malassezia species, and to explore the relationship between RAPD patterns and Malassezia species. Methods A total of 47 Malassezia isolates were obtained from 34 patients with pityriasis versicolor, and they were classified into 5 species by morphological, physiological and biochemical features, I.e., M. Fin'fur, M. Obtusa, M. Globosa, M. Restricta and M. Sympodialis. Genomic DNA was extracted from the 47 clinical isolates and 10 reference strains (including 7 species) of Malassezia. PCR was performed using 4 random primers including S22, S24, S25 and S33. RAPD patterns were analyzed by NTSYS

  18. Research of the Process of Coriolus Versicolor Strain V1 by Liquid Fermentation%云芝菌株CV1液体发酵的工艺研究

    Institute of Scientific and Technical Information of China (English)

    崔细鹏; 王敏; 史宝军

    2013-01-01

      利用CV1菌株液体发酵生产云芝多糖,分别考察培养基碳源、培养基氮源、碳源浓度、氮源浓度、无机盐对该菌株产云芝多糖性能的影响,采用正交实验法确立了云芝CV1产胞外多糖的最佳培养基:蔗糖:4%,大豆粉:5%;KH2PO4:0.1%;MgSO4:0.1%;在摇床转速:200r/min、发酵温度:26-28℃、发酵周期:65h、培养基初始pH值:自然条件下云芝CV1的胞外粗多糖(EPS)含量可达7.458g/L,菌丝体(Biomass)可达64.619g/L.%Use CV1 strain liquid fermentation producing polysaccharide of the Coriolus versicolor , separately in-spected medium carbon source, medium nitrogen source, carbon source concentration and nitrogen source, inorganic salt concentration of the strains producing the effect on the properties of polysaccharides, using the orthogonal ex-periment method established CV1 produce extracellular polysaccharide the best medium: cane sugar 4%, soybean meal 5%, KH2PO4 0.1%, MgSO4 0.1%, 200rpm, 26-28℃, 65h, medium initial pH value: Natural conditions CV1 of extracellular polysaccharide (EPS) content can reach 7.458g/L, Biomass can reach 64.619g/L.

  19. Nested PCR analysis for malassezia microflora from the scales of patients with pityriasis versicolor%花斑癣患者皮屑中马拉色菌的巢式PCR法分析

    Institute of Scientific and Technical Information of China (English)

    赵秀红; 冉玉平; 熊琳; 周光平; 山崎; 坪井良治; 小川秀兴

    2004-01-01

    目的用巢式PCR法检测马拉色菌特异的DNA从而快速准确的检测并鉴定马拉色菌的各菌种,并应用于花斑癣(Pityriasis Versicolor,PV)患者皮屑中马拉色菌携带情况中的研究.方法从PV患者的皮屑中提取马拉色菌的DNA,以马拉色菌属rRNA内转录间隔区设计属的引物和各个种的特异性引物分别进行巢式多聚酶链反应(nestPCR),检测105例PV患者皮屑中的马拉色菌的目的DNA片断.结果105例PV患者共检测到5例种的共321株马拉色菌的DNA.每例患者平均检测到约3种马拉色菌的DNA.各种马拉色菌的检出率依次为:球形马拉色菌80.0%,限制马拉色菌81.9%,合轴马拉色菌77.1%,糠秕马拉色菌41.9%,钝形马拉色菌15.2%.未检测到厚皮马拉色菌和斯洛菲马拉色菌.结论PV皮屑中存在多种马拉色菌,球形马拉色菌、合轴马拉色菌和限制马拉色菌是PV患者皮损处的主要马拉色菌种.

  20. Morphological and genetic variation in Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae, Cicindelinae: implications for conservation

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    Serrano, A.

    2003-12-01

    Full Text Available The establishment of taxonomic and geographical boundaries is a common problem when analysing clinal distributions. This is of particular concern when the assessment of intraspecific groupings is required for conservation management. The tiger beetle Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae is a typical case in which two recognised subspecies are distributed in a clinal latitudinal fashion in the dune systems along the Atlantic coast of Portugal. This habitat is increasingly under threat, and conservation measures are needed. We investigated the validity of the two named subspecies, based on a re-analysis of elytral and genitalic measurements using multivariate analysis. We also analysed variation in mitochondrial Cytochrome Oxidase I gene for a total of six populations along the cline. Multivariate analysis supported the idea of a morphological cline and revealed a clear distinction of the southernmost population and also some degree of distinctiveness of the most northern populations, partially supporting the recognised subspecific ranking. The mtDNA analysis identified two main groups corresponding to northern and southern populations. Both sets of markers showed that variation within the C. lusitanica assemblage is complex, with the boundaries between morphological and mtDNA groups not in agreement. However, populations at either end of the distributional range are clearly distinct from each other, and should be considered as provisional units for conservation programmes.El reconocimiento de límites taxonómicos y geográficos de la variabilidad observada es un problema habitual cuando se analizan distribuciones clinales. Esto es particularmente problemático cuando se requiere la determinación de agrupamientos intraespecíficos para tomar medidas de conservación. El cicindélido Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae constituye un caso típico en el que dos subespecies reconocidas se hayan distribuidas a

  1. Riqueza del complejo chisa (Coleoptera: Melolonthidae en cuatro agroecosistemas del Cauca, Colombia

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    Morón R. Miguel A.

    2005-12-01

    Full Text Available La composición y variación de la riqueza del complejo de larvas Melolonthidae se examinó en cuatro circunstancias agroecológicas en los municipios de Caldono y Buenos Aires, Cauca (1400 a 1500 m.s.n.m, 21,5ºC. y 2000 mm/año de precipitación promedio. Quincenalmente, durante un año, se visitaron parcelas de media hectárea de yuca, pastizal, cafetal y bosque, en cada parcela y ocasión se realizaron 10 muestreos ( 1m2 por 15 cm de profundidad. Se colectaron 10.261 larvas pertenecientes a 32 especies y 12 géneros de Melolonthidae, así: Phyllophaga, Plectris, Astaena, Macrodactylus, Ceraspis, Barybas, Isonychus, Anomala Callistethus, Strigoderma, Leucothyreus, Cyclocephala. La riqueza de especies presentó variaciones estadísticamente significativas entre localidades (F=87.24 p =2.72-18, muestreos (F=22.29 p =5.18-13, parcelas (F= 23.39 p=1.40-13 así como entre la mayoría de interacciones. Los promedios de riqueza fueron: yuca (4.5 , pastizal (3.6, café (2.1 y bosque (2.2, cada uno con grandes fluctuaciones que hicieron la diferencia. Se discuten las curvas de isodensidades de cada parcela y sus implicaciones en el programa de manejo. Abstract Richness of the white-grub complex (Coleoptera: Melolonthidae in agroecosystems of the Cauca province, Colombia. The composition and variation in the richness of the complex of rhizophagous Melolonthidae larvae was examinated in four agroecological zones in the Municipality of Caldono (1400-1500 m, 21.5ºC, average rainfall 2000 mm/yr with complementary surveys in the neighbouring site of Buenos Aires, Cauca. Half-hectare plots (cassava, pastures, coffee and wooded areas were selected and visited every 15 days (total of 15 visits per plot, and a total of 10 samples were taken (1 m2 by 15 cm deep. In total, 10,261 larvae of 32 species were collected: Phyllophaga, Plectris, Astaena, Macrodactylus, Ceraspis, Barybas, Isonychus, Anomala Callistethus, Strigoderma, Leucothyreus, Cyclocephala

  2. Typhlocharis vicariantes del Estrecho de Gibraltar. I: Typhlocharis armata Coiffait, 1969 (Coleoptera, Caraboidea, Trechidae

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    Pérez González, S.

    2010-12-01

    Full Text Available This first part about the vicariant Typhlocharis from Gibraltar Strait is dedicated to Typhlocharis armata Coiffait, 1969; a second paper will cover T. silvanoides Dieck, 1869. The studied material was obtained in four field campaigns carried out in the south of Spain and north of Morocco between 1997 and 2001 (Zaballos & Banda, 2000; Zaballos, 2005. In these campaigns abundant specimens of Typhlocharis (Coleoptera, Caraboidea, Trechidae were collected in different localities, the majority of them belonging to the species T. armata Coiffait, 1969. Their study and the comparison with the type series of this species allowed its complete redescription and clarification of its status within the genus. Contrary to what has been considered traditionally, the marginal umbilicate series of elytra in T. armata is composed of three, instead of four posterior setae. The taxonomical affinities of this species are established, the morphological weight of the umbilicate group of elytral setae as a lineage and species group defining character is discussed, and the theory of Jeanne (1973 about the relation between shortening of elytra and umbilicate series reduction with a decrease in body size is revised. The dispersal abilities are evaluated and a biogeographical explanation for the new distribution of the species is proposed.Esta primera parte sobre las especies de Typhlocharis vicariantes del Estrecho de Gibraltar está dedicada a Typhlocharis armata Coiffait, 1969; mientras que en un segundo artículo se tratará T. silvanoides Dieck, 1869. El material estudiado procede de cuatro campañas entomológicas realizadas en el sur de España y en el norte de Marruecos entre 1997 y 2001 (Zaballos & Banda, 2000; Zaballos, 2005. En estas campañas se recolectaron, en localidades diferentes, abundantes ejemplares de Typhlocharis (Coleoptera, Caraboidea, Trechidae, la mayoría de los cuales han resultado pertenecer a la especie T. armata Coiffait, 1969. Su estudio y

  3. Contributions towards an understanding of the Atomariinae (Coleoptera, Cryptophagidae of Atlantic Canada

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    Christopher Majka

    2010-02-01

    Full Text Available The Atomariinae (Coleoptera: Cryptophagidae fauna of Atlantic Canada are surveyed. Ten species in the genus Atomaria are reported in the region, six of which, including Atomaria distincta Casey, Atomaria fuscata Schonherr, Atomaria lederi Johnson, Atomaria lewisi Reitter, Atomaria testacea Stephens, and Atomaria nigrirostris Stephens, are newly recorded in Canada. Atomaria lederi and A. lewisi are newly recorded in North America. Twenty-three new provincial records are reported. Atomaria curtula Casey, 1900  (syn. n. is designated as a synonym of Atomaria lewisi Reitter; Atomaria ovalis Casey, 1900 (syn. n. is designated as a synonym of Atomaria apicalis Erichson, 1846; and Atomaria saginata Casey, 1900 (syn. n. is designated as a synonym of Atomaria fuscata Schonherr. A key to species is provided, as are species descriptions. Records of all species are given and their distribution in the region is plotted. The global distribution of all Holarctic and adventive species is briefly summarized, and the jurisdictions in North America where they have been reported are compiled. Habitat and bionomic information is also provided for all species, both from within the region, and from observations in European studies. The fauna is briefly discussed from a zoogeographic perspective; two species are Nearctic in origin, five may be Holarctic in distribution, and three others may be adventive Palaearctic species, although further evidence is required to confirm such preliminary assessments. Similarly, because of the paucity of information on this genus, further fieldwork is required to fully discern the distribution of these spe- cies in Atlantic Canada. Three are apparently saproxylic species characteristic of forested habitats; three are primarily grassland species; and the remaining four are eurytopic species found in a wide variety of forested and open habitats.

  4. Flight behaviour and dispersal of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) adults using mark-release-recapture method.

    Science.gov (United States)

    Ávalos, J A; Balasch, S; Soto, A

    2016-10-01

    The flight ability and patterns of an insect influence its spread, and the study of its behaviour can be used to improve the strategies to control the pest. Regarding Rhynchophorus ferrugineus (Olivier) (Coleoptera: Dryophthoridae), one of the worst threats to palm trees worldwide, laboratory experiments have been conducted to analyze their flight potential. However, these data must be complemented with tests that allow us to know its flight behaviour and dispersal patterns under field conditions. Two mark-release-recapture experiments were conducted in areas with R. ferrugineus infestations. In the first, the effects of weevil sex, temperature, solar radiation, and relative humidity, on the take-off and flight mobility of adults were analyzed. The second experiment aimed to determine the maximum flight distance covered by adults in field. The take-off rate for R. ferrugineus males was significantly greater than for females, and was positively influenced by temperature (optimum take-off around 25°C) and solar radiation, both factors being highly correlated. Female weevil recaptures were significantly higher, especially as temperatures increased (optimum recapture around 21°C). Dispersal distances of weevil adults increased when temperatures rose, and while this insect tended to fly short distances (<500 m), it was able to cover up to 7 km. The dispersal of R. ferrugineus adults mainly occurred during the first 7 days after their release, and when relative humidity increased, their dispersal time was reduced. The results obtained will permit a more effective implementation of certain measures used to control R. ferrugineus, such as olfactory trapping or intensive surveillance around pest outbreaks.

  5. Organization and comparative analysis of the mitochondrial genomes of bioluminescent Elateroidea (Coleoptera: Polyphaga).

    Science.gov (United States)

    Amaral, Danilo T; Mitani, Yasuo; Ohmiya, Yoshihiro; Viviani, Vadim R

    2016-07-25

    Mitochondrial genome organization in the Elateroidea superfamily (Coleoptera), which include the main families of bioluminescent beetles, has been poorly studied and lacking information about Phengodidae family. We sequenced the mitochondrial genomes of Neotropical Lampyridae (Bicellonycha lividipennis), Phengodidae (Brasilocerus sp.2 and Phrixothrix hirtus) and Elateridae (Pyrearinus termitilluminans, Hapsodrilus ignifer and Teslasena femoralis). All species had a typical insect mitochondrial genome except for the following: in the elaterid T. femoralis genome there is a non-coding region between NADH2 and tRNA-Trp; in the phengodids Brasilocerus sp.2 and P. hirtus genomes we did not find the tRNA-Ile and tRNA-Gln. The P. hirtus genome showed a ~1.6kb non-coding region, the rearrangement of tRNA-Tyr, a new tRNA-Leu copy, and several regions with higher AT contents. Phylogenetics analysis using Bayesian and ML models indicated that the Phengodidae+Rhagophthalmidae are closely related to Lampyridae family, and included Drilus flavescens (Drilidae) as an internal clade within Elateridae. This is the first report that compares the mitochondrial genomes organization of the three main families of bioluminescent Elateroidea, including the first Neotropical Lampyridae and Phengodidae. The losses of tRNAs, and translocation and duplication events found in Phengodidae mt genomes, mainly in P. hirtus, may indicate different evolutionary rates in these mitochondrial genomes. The mitophylogenomics analysis indicates the monophyly of the three bioluminescent families and a closer relationship between Lampyridae and Phengodidae/Rhagophthalmidae, in contrast with previous molecular analysis. PMID:27060405

  6. Side-effects of glyphosate on the life parameters of Eriopis connexa (Coleoptera: Coccinelidae) in Argentina.

    Science.gov (United States)

    Mirande, L; Haramboure, M; Smagghe, G; Piñeda, S; Schneider, M I

    2010-01-01

    In Argentina, transgenic soybean crop (Roundup Ready, RR) has undergone a major expansion over the last 15 years, with the consequent increase of glyphosate applications, a broad-spectrum and post emergence herbicide. Soybean crops are inhabited by several arthropods. Eriopis connexa Germar (Coleoptera: Coccinelidae) is a predator associated to soybean soft-bodies pest and have a Neotropical distribution. Nowadays, it is being considered a potentially biological control agent in South America. The objectives of this work were to evaluate the side-effects of glyphosate on larvae (third instar) and adults of this predator. Commercial compound and the maximum registered concentrations for field use were employed: GlifoGlex 48 (48% glyphosate, 192 mg a.i./litre, Gleba Argentina S.A.). The exposure was by ingestion through the treated prey (Rophalosiphum padi) or by drinking treated water during 48 h for treatment of the adult. The herbicide solutions were prepared using distilled water as solvent. The bioassays were carried out in the laboratory under controlled conditions: 23 +/- 0.5 degrees C, 75 +/- 5% RH and 16:8 (L:D) of photoperiod. Development time, weight of pupae, adult emergence, pre-oviposition period, fecundity and fertility were evaluated as endpoints. Larvae from glyphosate treatment molted earlier than controls. In addition, the weight of pupae, longevity, fecundity and fertility were drastically reduced in treated organisms. The reductions were more drastic when the treatments were performed at the third larval stage than as adult. The reproduction capacity of the predator was the most affected parameter and could be related to a hormonal disruption by glyphosate in the treated organisms. This work can confirm the deleterious effects of this herbicide on beneficial organisms. Also, it agrees with prior studies carried out on other predators associated to soybean pest, such as Chrysoperla externa (Neuroptera: Chrysopidae) and Alpaida veniliae (Araneae

  7. Exploring the Leaf Beetle Fauna (Coleoptera: Chrysomelidae of an Ecuadorian Mountain Forest Using DNA Barcoding.

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    Birthe Thormann

    Full Text Available Tropical mountain forests are hotspots of biodiversity hosting a huge but little known diversity of insects that is endangered by habitat destruction and climate change. Therefore, rapid assessment approaches of insect diversity are urgently needed to complement slower traditional taxonomic approaches. We empirically compare different DNA-based species delimitation approaches for a rapid biodiversity assessment of hyperdiverse leaf beetle assemblages along an elevational gradient in southern Ecuador and explore their effect on species richness estimates.Based on a COI barcode data set of 674 leaf beetle specimens (Coleoptera: Chrysomelidae of 266 morphospecies from three sample sites in the Podocarpus National Park, we employed statistical parsimony analysis, distance-based clustering, GMYC- and PTP-modelling to delimit species-like units and compared them to morphology-based (parataxonomic species identifications. The four different approaches for DNA-based species delimitation revealed highly similar numbers of molecular operational taxonomic units (MOTUs (n = 284-289. Estimated total species richness was considerably higher than the sampled amount, 414 for morphospecies (Chao2 and 469-481 for the different MOTU types. Assemblages at different elevational levels (1000 vs. 2000 m had similar species numbers but a very distinct species composition for all delimitation methods. Most species were found only at one elevation while this turnover pattern was even more pronounced for DNA-based delimitation.Given the high congruence of DNA-based delimitation results, probably due to the sampling structure, our study suggests that when applied to species communities on a regionally limited level with high amount of rare species (i.e. ~50% singletons, the choice of species delimitation method can be of minor relevance for assessing species numbers and turnover in tropical insect communities. Therefore, DNA-based species delimitation is confirmed as a

  8. Interactions between imidacloprid and Metarhizium brunneum on adult Asian longhorned beetles (Anoplophora glabripennis (Motschulsky)) (Coleoptera: Cerambycidae).

    Science.gov (United States)

    Russell, Calum W; Ugine, Todd A; Hajek, Ann E

    2010-11-01

    Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae), a longhorned beetle species native to Asia, has been introduced into several North American and European cities. Currently eradication and preventive measures are limited to identifying and destroying infested trees and protecting uninfested trees with trunk or soil-injections of the systemic insecticide imidacloprid. Because entomopathogenic fungi like Metarhizium brunneum Petch have been identified as virulent against these beetles we conducted several tests to determine the compatibility of the two agents in combination. Radial hyphal growth and the sporulation capacity of M. brunneum on Sabouraud dextrose agar with yeast were not significantly affected by the presence of imidacloprid. In a 2×3 factorial experiment investigating interactions between exposure to imidacloprid and M. brunneum we observed no effect of imidacloprid alone on beetle survival when beetles were given a single dose of 10 or 100 ppm compared to control insects. We observed a significant effect of exposure to M. brunneum, and a significant interaction between imidacloprid and M. brunneum representing a synergistic effect of dual treatment. Beetles exposed to the fungus alone lived significantly longer compared to insects treated with a single dose of 100 ppm imidacloprid (9.5 vs. 6.5d). Consumption of striped maple twigs by beetles exposed to imidacloprid, across concentrations, was reduced 48% compared to control insects, where as consumption by M. brunneum-exposed beetles was reduced by 16% over the first 6-days of the test period. Beetles fed 100 ppm imidacloprid consumed 32% less over the first 3d compared to beetles not exposed to imidacloprid and thereafter consumed as much as beetles not fed 100 ppm imidacloprid. M. brunneum-exposed beetles consumed significantly less food than control insects throughout the test period, and beetles treated with imidacloprid produced significantly fewer conidia compared to beetles

  9. Sperm bundle and reproductive organs of carabid beetles tribe Pterostichini (Coleoptera: Carabidae)

    Science.gov (United States)

    Sasakawa, Kôji

    2007-05-01

    The morphological characteristics of sperm and reproductive organs may offer clues as to how reproductive systems have evolved. In this paper, the morphologies of the sperm and male reproductive organs of carabid beetles in the tribe Pterostichini (Coleoptera: Carabidae) are described, and the morphological associations among characters are examined. All species form sperm bundles in which the head of the sperm was embedded in a rod-shaped structure, i.e., spermatodesm. The spermatodesm shape (left-handed spiral, right-handed spiral, or without conspicuous spiral structure) and the condition of the sperm on the spermatodesm surface (with the tail free-moving or forming a thin, sheetlike structure) vary among species. In all species, the spiral directions of the convoluted seminal vesicles and vasa deferentia are the same on both sides of the body; that is, they show an asymmetric structure. The species in which the sperm bundle and the seminal vesicles both have a spiral structure could be classified into two types, with significant differences in sperm-bundle length between the two types. The species with a sperm-bundle spiral and seminal-vesicle spiral of almost the same diameter have longer sperm bundles than the species with a sperm-bundle spiral and seminal-vesicle tube of almost the same diameter. In the former type, the spiral directions of the sperm bundles and seminal vesicles are inevitably the same, whereas they differ in some species with the later type. Therefore, increased sperm bundle length appears to have been facilitated by the concordance of the sperm bundle’s coiling direction with the coiling direction of the seminal vesicle.

  10. A comparison of trap type and height for capturing cerambycid beetles (Coleoptera).

    Science.gov (United States)

    Graham, Elizabeth E; Poland, Therese M; McCullough, Deborah G; Millar, Jocelyn G

    2012-06-01

    Wood-boring beetles in the family Cerambycidae (Coleoptera) play important roles in many forest ecosystems. However, increasing numbers of invasive cerambycid species are transported to new countries by global commerce and threaten forest health in the United States and worldwide. Our goal was to identify effective detection tools for a broad array of cerambycid species by testing some known cerambycid attractants and a pheromone in different trap designs placed across a range of habitats. We compared numbers and species richness of cerambycid beetles captured with cross-vane panel traps and 12-unit Lindgren multiple-funnel traps, placed either at ground level (1.5 m high) or canopy level (approximately 3-10 m high), at eight sites classified as either residential, industrial, deciduous forest, or conifer forest. We captured 3,723 beetles representing 72 cerambycid species from 10 June to 15 July 2010. Species richness was highest for the subfamilies Cerambycinae and Lamiinae, which accounted for 33 and 46% of all species captured, respectively. Overall, the cross-vane panel traps captured approximately 1.5 times more beetles than funnel traps. Twenty-one species were captured exclusively in traps at one height, either in the canopy or at ground level. More species were captured in hardwood sites (59 species) where a greater diversity of host material was available than in conifer (34 species), residential (41 species), or industrial (49) sites. Low numbers of beetles (n < 5) were recorded for 28 of the beetle species. The number of species captured per week ranged from 49 species on 21 June to 37 species on 12 July. Cross-vane panel traps installed across a vertical gradient should maximize the number of cerambycid species captured.

  11. Experimental studies and dynamics modeling analysis of the swimming and diving of whirligig beetles (Coleoptera: Gyrinidae).

    Science.gov (United States)

    Xu, Zhonghua; Lenaghan, Scott C; Reese, Benjamin E; Jia, Xinghua; Zhang, Mingjun

    2012-01-01

    Whirligig beetles (Coleoptera, Gyrinidae) can fly through the air, swiftly swim on the surface of water, and quickly dive across the air-water interface. The propulsive efficiency of the species is believed to be one of the highest measured for a thrust generating apparatus within the animal kingdom. The goals of this research were to understand the distinctive biological mechanisms that allow the beetles to swim and dive, while searching for potential bio-inspired robotics applications. Through static and dynamic measurements obtained using a combination of microscopy and high-speed imaging, parameters associated with the morphology and beating kinematics of the whirligig beetle's legs in swimming and diving were obtained. Using data obtained from these experiments, dynamics models of both swimming and diving were developed. Through analysis of simulations conducted using these models it was possible to determine several key principles associated with the swimming and diving processes. First, we determined that curved swimming trajectories were more energy efficient than linear trajectories, which explains why they are more often observed in nature. Second, we concluded that the hind legs were able to propel the beetle farther than the middle legs, and also that the hind legs were able to generate a larger angular velocity than the middle legs. However, analysis of circular swimming trajectories showed that the middle legs were important in maintaining stable trajectories, and thus were necessary for steering. Finally, we discovered that in order for the beetle to transition from swimming to diving, the legs must change the plane in which they beat, which provides the force required to alter the tilt angle of the body necessary to break the surface tension of water. We have further examined how the principles learned from this study may be applied to the design of bio-inspired swimming/diving robots.

  12. Dung beetles (Coleoptera: Scarabaeoidea) in three landscapes in Mato Grosso do Sul, Brazil.

    Science.gov (United States)

    Rodrigues, M M; Uchôa, M A; Ide, S

    2013-02-01

    Dung beetles (Coleoptera: Scarabaeoidea) in three landscapes in Mato Grosso do Sul, Brazil. Dung Beetles are important for biological control of intestinal worms and dipterans of economic importance to cattle, because they feed and breed in dung, killing parasites inside it. They are also very useful as bioindicators of species diversity in agricultural or natural environments. The aims of this paper were to study the species richness, and abundance of dung beetles, helping to answer the question: are there differences in the patterns of dung beetle diversity in three environments (pasture, agriculture and forest) in the municipality of Dourados, in the state of Mato Grosso do Sul. A total of 105 samplings were carried out weekly, from November 2005 to November 2007, using three pitfall traps in each environment. The traps were baited with fresh bovine dung, and 44,355 adult dung beetles from 54 species were captured: two from Hyborosidae and 52 from Scarabaeidae. Five species were constant, very abundant and dominant on the pasture, two in the agricultural environment, and two in the environment of Semideciduous forest. Most of the species were characterised as accessories, common and not-dominant. The species with higher abundance was Ataenius platensis Blanchard, 1844. The indexes of Shannon-Wiener diversity were: 2.90 in the pasture, 2.84 in the agricultural environment and 2.66 in the area of native forest. The medium positive presence of dung beetles in the traps in each environment were: 36.88, 42.73 and 20.18 individuals per trap, in the pasture, agricultural environment and in the native forest, respectively. The pasture environment presented a higher diversity index. The species diversity of dung beetles was superior where there was higher abundance and regularity of resource (bovine dung).

  13. Observations on sex ratio and behavior of males in Xyleborinus saxesenii Ratzeburg (Scolytinae, Coleoptera

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    Peter Biedermann

    2010-09-01

    Full Text Available Strongly female-biased sex ratios are typical for the fungal feeding haplodiploid Xyleborini (Scolytinae, Coleoptera, and are a result of inbreeding and local mate competition (LMC. These ambrosia beetles are hardly ever found outside of trees, and thus male frequency and behavior have not been addressed in any empirical studies to date. In fact, for most species the males remain undescribed. Data on sex ratios and male behavior could, however, provide important insights into the Xyleborini’s mating system and the evolution of inbreeding and LMC in general. In this study, I used in vitro rearing methods to obtain the first observational data on sex ratio, male production, male and female dispersal, and mating behavior in a xyleborine ambrosia beetle. Females of Xyleborinus saxesenii Ratzeburg produced between 0 and 3 sons per brood, and the absence of males was relatively independent of the number of daughters to be fertilized and the maternal brood sex ratio. Both conformed to a strict LMC strategy with a relatively precise and constant number of males. If males were present they eclosed just before the first females dispersed, and stayed in the gallery until all female offspring had matured. They constantly wandered through the gallery system, presumably in search of unfertilized females, and attempted to mate with larvae, other males, and females of all ages. Copulations, however, only occurred with immature females. From galleries with males, nearly all females dispersed fertilized. Only a few left the natal gallery without being fertilized, and subsequently went on to produce large and solely male broods. If broods were male-less, dispersing females always failed to found new galleries.

  14. Variability in Small Hive Beetle (Coleoptera: Nitidulidae) Reproduction in Laboratory and Field Experiments.

    Science.gov (United States)

    Meikle, William G; Holst, Niels; Cook, Steven C; Patt, Joseph M

    2015-06-01

    Experiments were conducted to examine how several key factors affect population growth of the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae). Laboratory experiments were conducted to examine effects of food quantity and temperature on reproduction of cohorts of young A. tumida adults (1:1 sex ratio) housed in experimental arenas. Daily numbers and total mass of larvae exiting arenas were highly variable within treatment. Either one or two cohorts of larvae were observed exiting the arenas. Food quantity, either 10 g or 20 g, did not significantly affect the number of larvae exiting arenas at 32°C, but did at 28°C; arenas provided 20 g food produced significantly more larvae than arenas provided 10 g. Temperature did not affect the total mass of larvae provided 10 g food, but did affect larval mass provided 20 g; beetles kept at 28°C produced more larval mass than at 32°C. Field experiments were conducted to examine A. tumida reproductive success in full strength bee colonies. Beetles were introduced into hives as egg-infested frames and as adults, and some bee colonies were artificially weakened through removal of sealed brood. Efforts were unsuccessful; no larvae were observed exiting from, or during the inspection of, any hives. Possible reasons for these results are discussed. The variability observed in A. tumida reproduction even in controlled laboratory conditions and the difficulty in causing beetle infestations in field experiments involving full colonies suggest that accurately forecasting the A. tumida severity in such colonies will be difficult. PMID:26470208

  15. Comparative resistance of Russian and Italian honey bees (Hymenoptera: Apidae) to small hive beetles (Coleoptera: Nitidulidae).

    Science.gov (United States)

    Frake, Amanda M; De Guzman, Lilia I; Rinderer, Thomas E

    2009-02-01

    To compare resistance to small hive beetles (Coleoptera: Nitidulidae) between Russian and commercial Italian honey bees (Hymenoptera: Apidae), the numbers of invading beetles, their population levels through time and small hive beetle reproduction inside the colonies were monitored. We found that the genotype of queens introduced into nucleus colonies had no immediate effect on small hive beetle invasion. However, the influence of honey bee stock on small hive beetle invasion was pronounced once test bees populated the hives. In colonies deliberately freed from small hive beetle during each observation period, the average number of invading beetles was higher in the Italian colonies (29 +/- 5 beetles) than in the Russian honey bee colonies (16 +/- 3 beetles). A similar trend was observed in colonies that were allowed to be freely colonized by beetles throughout the experimental period (Italian, 11.46 +/- 1.35; Russian, 5.21 +/- 0.66 beetles). A linear regression analysis showed no relationships between the number of beetles in the colonies and adult bee population (r2 = 0.1034, P = 0.297), brood produced (r2 = 0.1488, P = 0.132), or amount of pollen (P = 0.1036, P = 0.295). There were more Italian colonies that supported small hive beetle reproduction than Russian colonies. Regardless of stock, the use of entrance reducers had a significant effect on the average number of small hive beetle (with reducer, 16 +/- 3; without reducer, 27 +/- 5 beetles). However, there was no effect on bee population (with reducer, 13.20 +/- 0.71; without reducer, 14.60 +/- 0.70 frames) or brood production (with reducer, 6.12 +/- 0.30; without reducer, 6.44 +/- 0.34 frames). Overall, Russian honey bees were more resistant to small hive beetle than Italian honey bees as indicated by fewer invading beetles, lower small hive beetle population through time, and lesser reproduction. PMID:19253612

  16. The presence of a mycangium in European Sinodendron cylindricum (Coleoptera: Lucanidae) and the associated yeast symbionts.

    Science.gov (United States)

    Tanahashi, Masahiko; Hawes, Colin J

    2016-01-01

    Part of the exoskeleton of some wood-inhabiting insects is modified to form a mycangium, which is a specialized organ used to convey fungal spores or yeasts to their offspring. Although most stag beetles (Coleoptera: Lucanidae) are known to have female-specific mycangia and associated yeast symbionts, the evolutionary origin of the mycangium in this group remains unresolved. Here, we report the presence of a mycangium and associated yeast symbionts in the European horned stag beetle Sinodendron cylindricum (L.), which belongs to an ancestral clade of the Lucanidae. The mycangium of S. cylindricum is shown to be female-specific and have the same developmental origin as that of other stag beetles. A total of five yeast strains were isolated from adult mycangia and larval gut of S. cylindricum Of these, we suggest that SICYAM1 is an undescribed yeast with taxonomic novelty, and have identified SICYLG3 as the xylose-fermenting yeast Scheffersomyces insectosa using nuclear ribosomal RNA and ITS sequences. The remaining three yeast strains, SICYAM2, SICYLG1, and SICYLG2, were assigned to the genus Sugiyamaella Yeast density in the adult mycangium was lower than that of the more evolutionarily advanced stag beetles, the European Lucanus cervus (L.) and Dorcus parallelipipedus (L.), which were also examined in this study. No living yeasts were isolated from the adult guts. However, a third instar larva of S. cylindricum harbored 10(4)-10(6) living yeasts in each gut region, which suggests that gut yeasts play an important role in these wood-feeding larvae. PMID:27432353

  17. Displacement of Tetropium cinnamopterum (Coleoptera: Cerambycidae) by Its Invasive Congener Tetropium fuscum.

    Science.gov (United States)

    Dearborn, Kenneth W; Heard, Stephen B; Sweeney, Jon; Pureswaran, Deepa S

    2016-08-01

    We examined the native community of insects interacting with an invasive species, Tetropium fuscum (F.) (Coleoptera: Cerambycidae), in its new range to explore reasons for the invader's relatively slow spread. Tetropium fuscum is a European spruce borer established in Nova Scotia since at least 1990, but it has spread only about 125 km from its site of introduction. We compared the densities of Tetropium spp., their known parasitoids, and the community of wood-boring insects at sites located within the invasion zone in Nova Scotia versus well outside this zone, in New Brunswick, Canada. Using red spruce trees stressed by girdling or felling, we tested whether: 1) T. fuscum had altered the native wood-boring community; 2) T. fuscum displaced a native congener, Tetropium cinnamopterum (Kirby); and 3) parasitism rates of Tetropium spp. differed between the invaded and noninvaded zones. Both Tetropium spp. and their parasitoid wasps emerged exclusively from felled trees as opposed to girdled trees. We found no difference in community diversity inside versus outside the invasion zone. The combined densities of both Tetropium spp. and their overall parasitism rates also did not differ between zones, but T. cinnamopterum density was significantly greater outside the invasion zone, suggesting T. fuscum may displace the native congener where they are sympatric. Our results suggest that the native and invasive Tetropium spp. act as a single functional species in the invasion zone. We speculate that natural control agents (predators, parasitoids, and competitors) might be limiting the rate of spread of T. fuscum. PMID:27247305

  18. Endophyte isolate and host grass effects on Chaetocnema pulicaria (Coleoptera: Chrysomelidae) feeding.

    Science.gov (United States)

    Ball, Olivier J P; Gwinn, Kimberly D; Pless, Charles D; Popay, Alison J

    2011-04-01

    Endophytic fungi belonging to the genus Neotyphodium, confer resistance to infected host grasses against insect pests. The effect of host species, and endophtye species and strain, on feeding and survival of the corn flea beetle, Chaetocnema pulicaria Melsheimer (Coleoptera: Chrysomelidae) was investigated. The grass-endophyte associations included natural and artificially derived associations producing varying arrays of common endophyte-related alkaloids or alkaloid groups, peramine, lolitrem B, ergovaline, and the lolines. Preference and nonpreference tests showed that C. pulicaria feeding and survival were reduced by infection of tall fescue with the wild-type strain of N. coenophialum, the likely mechanism being antixenosis rather than antibiosis. In the preference tests, endophyte and host species effects were observed. Of the 10 different Neotyphodium strains tested in artificially derived tall fescue associations, eight strongly deterred feeding by C. pulicaria, whereas the remaining two strains had little or no effect on feeding. Infection of tall fescue with another fungal symbiont, p-endophyte, had no effect. Perennial ryegrass, Lolium perenne L., infected with six strains of endophyte, was moderately resistant to C. pulicaria compared with endophyte-free grass, but four additional strains were relatively inactive. Six Neotyphodium-meadow fescue, Festuca pratensis Huds., associations, including the wild-type N. uncinatum-meadow fescue combination, were resistant, whereas three associations were not effective. Loline alkaloids seemed to play a role in antixenosis to C. pulicaria. Effects not attributable to the lolines or any other of the alkaloids examined also were observed. This phenomenon also has been reported in tests with other insects, and indicates the presence of additional insect-active factors.

  19. The presence of a mycangium in European Sinodendron cylindricum (Coleoptera: Lucanidae) and the associated yeast symbionts

    Science.gov (United States)

    Tanahashi, Masahiko; Hawes, Colin J.

    2016-01-01

    Part of the exoskeleton of some wood-inhabiting insects is modified to form a mycangium, which is a specialized organ used to convey fungal spores or yeasts to their offspring. Although most stag beetles (Coleoptera: Lucanidae) are known to have female-specific mycangia and associated yeast symbionts, the evolutionary origin of the mycangium in this group remains unresolved. Here, we report the presence of a mycangium and associated yeast symbionts in the European horned stag beetle Sinodendron cylindricum (L.), which belongs to an ancestral clade of the Lucanidae. The mycangium of S. cylindricum is shown to be female-specific and have the same developmental origin as that of other stag beetles. A total of five yeast strains were isolated from adult mycangia and larval gut of S. cylindricum. Of these, we suggest that SICYAM1 is an undescribed yeast with taxonomic novelty, and have identified SICYLG3 as the xylose-fermenting yeast Scheffersomyces insectosa using nuclear ribosomal RNA and ITS sequences. The remaining three yeast strains, SICYAM2, SICYLG1, and SICYLG2, were assigned to the genus Sugiyamaella. Yeast density in the adult mycangium was lower than that of the more evolutionarily advanced stag beetles, the European Lucanus cervus (L.) and Dorcus parallelipipedus (L.), which were also examined in this study. No living yeasts were isolated from the adult guts. However, a third instar larva of S. cylindricum harbored 104–106 living yeasts in each gut region, which suggests that gut yeasts play an important role in these wood-feeding larvae. PMID:27432353

  20. Modeling evolution of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) to transgenic corn with two insecticidal traits.

    Science.gov (United States)

    Onstad, David W; Meinke, Lance J

    2010-06-01

    A simulation model of the population dynamics and genetics of western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), was created to evaluate the use of refuges in the management of resistance to transgenic insecticidal corn, Zea mays L., expressing one or two toxin traits. Hypothetical scenarios and a case study of a corn hybrid pyramided with existing toxins are simulated. In the hypothetical situations, results demonstrated that evolution is generally delayed by pyramids compared with deployment of a single-toxin corn hybrid. However, soil insecticide use in the refuge reduced this delay and quickened the evolution of resistance. Results were sensitive to the degree of male beetle dispersal before mating and to the effectiveness of both toxins in the pyramid. Resistance evolved faster as fecundity increased for survivors of insecticidal corn. Thus, effects on fecundity must be measured to predict which resistance management plans will work well. Evolution of resistance also occurred faster if the survival rate due to exposure to the two toxins was not calculated by multiplication of two independent survival rates (one for each insect gene) but was equivalent to the minimum of the two. Furthermore, when single-trait and pyramided corn hybrids were planted within rootworm-dispersal distance of each other, the toxin traits lost efficacy more quickly than they did in scenarios without single-trait corn. For the case study involving transgenic corn expressing Cry34/35Ab1 and Cry3Bb1, the pyramid delayed evolution longer than a single trait corn hybrid and longer than a sequence of toxins based on at least one resistance-allele frequency remaining below 50%. Results are discussed within the context of a changing transgenic corn marketplace and the landscape dynamics of resistance management.

  1. Experimental studies and dynamics modeling analysis of the swimming and diving of whirligig beetles (Coleoptera: Gyrinidae.

    Directory of Open Access Journals (Sweden)

    Zhonghua Xu

    Full Text Available Whirligig beetles (Coleoptera, Gyrinidae can fly through the air, swiftly swim on the surface of water, and quickly dive across the air-water interface. The propulsive efficiency of the species is believed to be one of the highest measured for a thrust generating apparatus within the animal kingdom. The goals of this research were to understand the distinctive biological mechanisms that allow the beetles to swim and dive, while searching for potential bio-inspired robotics applications. Through static and dynamic measurements obtained using a combination of microscopy and high-speed imaging, parameters associated with the morphology and beating kinematics of the whirligig beetle's legs in swimming and diving were obtained. Using data obtained from these experiments, dynamics models of both swimming and diving were developed. Through analysis of simulations conducted using these models it was possible to determine several key principles associated with the swimming and diving processes. First, we determined that curved swimming trajectories were more energy efficient than linear trajectories, which explains why they are more often observed in nature. Second, we concluded that the hind legs were able to propel the beetle farther than the middle legs, and also that the hind legs were able to generate a larger angular velocity than the middle legs. However, analysis of circular swimming trajectories showed that the middle legs were important in maintaining stable trajectories, and thus were necessary for steering. Finally, we discovered that in order for the beetle to transition from swimming to diving, the legs must change the plane in which they beat, which provides the force required to alter the tilt angle of the body necessary to break the surface tension of water. We have further examined how the principles learned from this study may be applied to the design of bio-inspired swimming/diving robots.

  2. Efficacy of natural products against Callosobruchus chinensis (Coleoptera: Bruchidae) in Nepal.

    Science.gov (United States)

    Regmi, Homan; Kafle, Lekhnath; Gc, Yubak Dhoj; Shih, Cheng Jen

    2012-06-01

    This study was conducted to determine the efficacy of local natural products against the beetle, Callosobruchus chinensis L. (Coleoptera: Bruchidae), in stored chickpea Cicer arietinum L. (Fabaceae) in Chitwan, Nepal. Five natural products and one synthetic pesticide (Malathion) and two storage regimes (aluminum sheet bin vs. jute bags with plastic lining) were tested for their effect on stored pulse with respect to damage by C. chinensis. The five natural products included Xanthoylum armatum DC (Rutaceae) fruit powder, Acorus calamus L. (Araceae) rhizome powder, Cinnamom camphora L. (Lauraceae) balls, oil of Sesamum indicum L. (Pedaliaceae), and leaf powder of Azadirachta indica A. Juss. (Meliaceae). Treatments of stored pulses with natural products or with Malathion all caused significantly higher mortality of C. chinensis at 15 d after treatment (DAT) than stored pulses receiving no treatments. The balls of C. camphora, rhizome powder of A. calamus and sesame oil outperformed all other treatments, including the Malathion at 45 and 75 DAT and resulted in significantly reduced damage or deterioration of stored pulses compared with other treatments. Storage regimes performed similarly, although the jute bags did protect seed integrity for some treatments. Our results indicate that incorporating these natural products into stored pulses can protect the seeds from C. chinensis for up to two generations, something that Malathion cannot do. These products are readily available to most farmers in the region and their use will lead to 1) reduction of losses to significant stored product pests, and 2) a reduction of contamination of foodstuffs and the environment by synthetic pesticides like Malathion. PMID:22812152

  3. Jumping mechanisms and performance in beetles. I. Flea beetles (Coleoptera: Chrysomelidae: Alticini).

    Science.gov (United States)

    Nadein, Konstantin; Betz, Oliver

    2016-07-01

    The present study analyses the anatomy, mechanics and functional morphology of the jumping apparatus, the performance and the kinematics of the natural jump of flea beetles (Coleoptera: Chrysomelidae: Galerucinae: Alticini). The kinematic parameters of the initial phase of the jump were calculated for five species from five genera (average values from minimum to maximum): acceleration 0.91-2.25 (×10(3)) m s(-2), velocity 1.48-2.80 m s(-1), time to take-off 1.35-2.25 ms, kinetic energy 2.43-16.5 µJ, G: -force 93-230. The jumping apparatus is localized in the hind legs and formed by the femur, tibia, femoro-tibial joint, modified metafemoral extensor tendon, extensor ligament, tibial flexor sclerite, and extensor and flexor muscles. The primary role of the metafemoral extensor tendon is seen in the formation of an increased attachment site for the extensor muscles. The rubber-like protein resilin was detected in the extensor ligament, i.e. a short, elastic element connecting the extensor tendon with the tibial base. The calculated specific joint power (max. 0.714 W g(-1)) of the femoro-tibial joint during the jumping movement and the fast full extension of the hind tibia (1-3 ms) suggest that jumping is performed via a catapult mechanism releasing energy that has beforehand been stored in the extensor ligament during its stretching by the extensor muscles. In addition, the morphology of the femoro-tibial joint suggests that the co-contraction of the flexor and the extensor muscles in the femur of the jumping leg is involved in this process. PMID:27385755

  4. Jumping mechanisms and performance in beetles. I. Flea beetles (Coleoptera: Chrysomelidae: Alticini).

    Science.gov (United States)

    Nadein, Konstantin; Betz, Oliver

    2016-07-01

    The present study analyses the anatomy, mechanics and functional morphology of the jumping apparatus, the performance and the kinematics of the natural jump of flea beetles (Coleoptera: Chrysomelidae: Galerucinae: Alticini). The kinematic parameters of the initial phase of the jump were calculated for five species from five genera (average values from minimum to maximum): acceleration 0.91-2.25 (×10(3)) m s(-2), velocity 1.48-2.80 m s(-1), time to take-off 1.35-2.25 ms, kinetic energy 2.43-16.5 µJ, G: -force 93-230. The jumping apparatus is localized in the hind legs and formed by the femur, tibia, femoro-tibial joint, modified metafemoral extensor tendon, extensor ligament, tibial flexor sclerite, and extensor and flexor muscles. The primary role of the metafemoral extensor tendon is seen in the formation of an increased attachment site for the extensor muscles. The rubber-like protein resilin was detected in the extensor ligament, i.e. a short, elastic element connecting the extensor tendon with the tibial base. The calculated specific joint power (max. 0.714 W g(-1)) of the femoro-tibial joint during the jumping movement and the fast full extension of the hind tibia (1-3 ms) suggest that jumping is performed via a catapult mechanism releasing energy that has beforehand been stored in the extensor ligament during its stretching by the extensor muscles. In addition, the morphology of the femoro-tibial joint suggests that the co-contraction of the flexor and the extensor muscles in the femur of the jumping leg is involved in this process.

  5. Relationships of abscised cotton fruit to boll weevil (Coleoptera: Curculionidae) feeding, oviposition, and development.

    Science.gov (United States)

    Showler, Allan T

    2008-02-01

    Abscised cotton, Gossypium hirsutum L., fruit in field plots planted at different times were examined to assess adult boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), use of squares and bolls during 2002 and 2003 in the Lower Rio Grande Valley of Texas. Although boll abscission is not necessarily related to infestation, generally more bolls abscised than squares and abundances of fallen bolls were not related to the planting date treatments. During 2003, fallen squares were most abundant in the late-planted treatment. Although large squares (5.5-8-mm-diameter) on the plant are preferred for boll weevil oviposition, diameter of abscised squares is not a reliable measurement because of shrinkage resulting from desiccation and larval feeding. Fallen feeding-punctured squares and bolls were most abundant in late plantings but differences between fallen feeding-punctured squares versus fallen feeding-punctured bolls were found in only one treatment in 2003. During the same year, fallen oviposition-punctured squares were more numerous in the late-planted treatment than in the earlier treatments. Treatment effects were not found on numbers of oviposition-punctured bolls, but fallen oviposition-punctured squares were more common than bolls in the late-planted treatment compared with earlier treatments each year. Dead weevil eggs, larvae, and pupae inside fallen fruit were few and planting date treatment effects were not detected. Living third instars and pupae were more abundant in fallen squares of the late-planted treatment than in the earlier treatments and bolls of all three treatments. This study shows that fallen squares in late-planted cotton contribute more to adult boll weevil populations than bolls, or squares of earlier plantings. PMID:18330118

  6. Influence of trap color and host volatiles on capture of the emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Crook, Damon J; Khrimian, Ashot; Cossé, Allard; Fraser, Ivich; Mastro, Victor C

    2012-04-01

    Field trapping assays were conducted in 2009 and 2010 throughout western Michigan, to evaluate lures for adult emerald ash borer, A. planipennis Fairmaire (Coleoptera: Buprestidae). Several ash tree volatiles were tested on purple prism traps in 2009, and a dark green prism trap in 2010. In 2009, six bark oil distillate lure treatments were tested against manuka oil lures (used in 2008 by USDA APHIS PPQ emerald ash borer cooperative program). Purple traps baited with 80/20 (manuka/phoebe oil) significantly increased beetle catch compared with traps baited with manuka oil alone. In 2010 we monitored emerald ash borer attraction to dark green traps baited with six lure combinations of 80/20 (manuka/phoebe), manuka oil, and (3Z)-hexenol. Traps baited with manuka oil and (3Z)-hexenol caught significantly more male and total count insects than traps baited with manuka oil alone. Traps baited with manuka oil and (3Z)-hexenol did not catch more beetles when compared with traps baited with (3Z)-hexenol alone. When compared with unbaited green traps our results show that (3Z)-hexenol improved male catch significantly in only one of three field experiments using dark green traps. Dark green traps caught a high number of A. planipennis when unbaited while (3Z)-hexenol was seen to have a minimal (nonsignificant) trap catch effect at several different release rates. We hypothesize that the previously reported kairomonal attractancy of (3Z)-hexenol (for males) on light green traps is not as obvious here because of improved male attractancy to the darker green trap. PMID:22606813

  7. Chemosensory gene families in adult antennae of Anomala corpulenta Motschulsky (Coleoptera: Scarabaeidae: Rutelinae.

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    Xiao Li

    Full Text Available The metallic green beetle, Anomala corpulenta (Coleoptera: Scarabaeidae: Rutelinae, is a destructive pest in agriculture and horticulture throughout Asia, including China. Olfaction plays a crucial role in the survival and reproduction of A. corpulenta. As a non-model species, A. corpulenta is poorly understood, and information regarding the molecular mechanisms underlying olfaction in A. corpulenta and other scarab species is scant.We assembled separate antennal transcriptome for male and female A. corpulenta using Illumina sequencing technology. The relative abundance of transcripts with gene ontology annotations, including those related to olfaction in males and females was highly similar. Transcripts encoding 15 putative odorant binding proteins, five chemosensory proteins, one sensory neuron membrane protein, 43 odorant receptors, eight gustatory receptors, and five ionotropic receptors were identified. The sequences of all of these chemosensory-related transcripts were confirmed using reverse transcription polymerase chain reaction (RT-PCR, and direct DNA sequencing. The expression patterns of 54 putative chemosensory genes were analyzed using quantitative real time RT-PCR (qRT-PCR. Antenna-specific expression was detected for many of these genes, suggesting that they may have important functions in semiochemical detection.The identification of a large number of chemosensory proteins provides a major resource for the study of the molecular mechanism of odorant detection in A. corpulenta and its chemical ecology. The genes identified, especially those that were expressed at high levels in the antennae may represent novel molecular targets for the development of population control strategies based on the manipulation of chemoreception-driven behaviors.

  8. A importância forense de Oxelytrum discicolle (Brullé, 1840 (Coleoptera, Silphidae

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    Camila Kraiczyi Kotzko

    2015-08-01

    Full Text Available Oxelytrum discicolle Brullé, 1840 é a espécie mais coletada de Silphidae (Coleoptera na América Latina, constituindo um indicador forense na região Neotropical. O objetivo do trabalho é reunir informações sobre biologia e distribuição de O. discicolle, além de ferramentas para utilização desse besouro como indicador forense em casos de morte de humanos. O. discicolle é um besouro de grande porte, de cor preta com um colar vermelho. Larvas da espécie são necrófagas, já os adultos são onívoros e se alimentam tanto de larvas de dípteros quanto da própria carcaça. Nossa revisão aponta que a espécie reduz seu tempo de desenvolvimento conforme a temperatura aumenta, sugerindo que a taxa de desenvolvimento é diferente entre as temperaturas. Estudos mostram que a espécie O. discicolle é um forte indicador forense, porém para que ocorra o avanço das pesquisas de Entomologia Forense em humanos no Brasil é necessário que os Institutos de Criminalística publiquem mais dados sobre insetos que os colonizam. Além disso, são necessários mais estudos em carcaças de animais em diversas regiões e biomas diferentes, desta forma pode ser montado um mapa de distribuição da espécie em nível nacional.

  9. Evaluation of heat treatment schedules for emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Myers, Scott W; Fraser, Ivich; Mastro, Victor C

    2009-12-01

    The thermotolerance of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), was evaluated by subjecting larvae and prepupae to a number of time-temperature regimes. Three independent experiments were conducted during 2006 and 2007 by heating emerald ash borer infested firewood in laboratory ovens. Heat treatments were established based on the internal wood temperature. Treatments ranged from 45 to 65 degrees C for 30 and 60 min, and the ability of larvae to pupate and emerge as adults was used to evaluate the success of each treatment. A fourth experiment was conducted to examine heat treatments on exposed prepupae removed from logs and subjected to ambient temperatures of 50, 55, and 60 degrees C for 15, 30, 45, and 60 min. Results from the firewood experiments were consistent in the first experiment. Emergence data showed emerald ash borer larvae were capable of surviving a temperatures-time combination up to 60 degrees C for 30 min in wood. The 65 degrees C for 30 min treatment was, however, effective in preventing emerald ash borer emergence on both dates. Conversely, in the second experiment using saturated steam heat, complete mortality was achieved at 50 and 55 degrees C for both 30 and 60 min. Results from the prepupae experiment showed emerald ash borer survivorship in temperature-time combinations up to 55 degrees C for 30 min, and at 50 degrees C for 60 min; 60 degrees C for 15 min and longer was effective in preventing pupation in exposed prepupae. Overall results suggest that emerald ash borer survival is variable depending on heating conditions, and an internal wood temperature of 60 degrees C for 60 min should be considered the minimum for safe treatment for firewood.

  10. Mass Trapping and Classical Biological Control of Rhynchophorus palmarum L. 1794 (Coleoptera: Curculionidae. A hypothesis based in morphological evidences.

    Directory of Open Access Journals (Sweden)

    Flávio Miguens

    2011-07-01

    Resumo. Coleópteros têm sido descritos como pragas e vetores de nematódeos causadores de Anel Vermelho em diversas palmeiras da família Arecaceae. Rhynchophorus palmarum L (Coleoptera: Curculionidae é uma praga que afeta a cocoicultura e outras palmeiras; e, vetor de Bursaphelencus cocophilus (Cobb Baujard (Nematoda, agente etiológico de Anel Vermelho e de outros nematódeos. Atualmente, recomenda-se o emprego de inimigos naturais e parasitas no manejo integrado de pragas; dentre elas, Rhynchophorinae. Armadilhas de coleta massal são recomendadas no manejo integrado de pragas. Nosso estudo relata, na cocoicultura, a eficiência de armadilhas artesanais de baixo custo e a utilização cariomônios (toletes de cana-de-açúcar e cariômonios mais feromônios (toletes de cana-de-açúcar e machos adultos de R. palmarum como atrativos nas armadilhas. Ácaros ectoparasitas foram identificados nestes coleópteros, por microscopia, que podem ser propostos como parte do manejo integrado desta praga. Armadilhas de coleta massal com cariômonios foram eficientes na captura de R. palmarum e outros Curculionidae. No entanto, armadilhas de coleta massal com cariômonios e feromônios aumentaram a atratividade, em relação às primeiras, para este Coleoptera e Cosmopolites sordidus (Germar (Coleoptera: Curculionidae. Eventualmente, exemplares de R. palmarum apresentavam infecção fúngica. Ácaros ectoparasitas infestavam mais de 50% dos exemplares de R. palmarum. A microanatomia destes ácaros e sua interação com R. palmarum foi preliminarmente descrita. Todos os estágios do ciclo de vida destes ácaros foram identificados no compartimento dos élitros. As evidências morfológicas suportam a hipótese de que estes ácaros podem ser empregados no controle biológico de R. palmarum em um programa de manejo integrado.

  11. Attraction of Astylus variegatus (Germ. (Coleoptera: Melyridae by volatile floral attractants Atração de Astylus variegatus (Germ. (Coleoptera: Melyridae por atraentes florais voláteis

    Directory of Open Access Journals (Sweden)

    Maurício Ursi Ventura

    2007-06-01

    Full Text Available The beetle Astylus variegatus (Germ. (Coleoptera: Melyridae is frequently found in flowers feeding on pollen. Responses of A. variegatus to volatile floral attractants were studied in common beans (Phaseolus vulgaris L. fields. Traps originally designed to capture Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, consisted of plastic bottles (2 L with 150 holes (5-mm diameter yellow gold painted and containing inside a plastic strip (3.5 ´ 25 cm with Lagenaria vulgaris (L. powder (0.28% B cucurbitacin - feeding stimulant and arrestant for diabroticites sprayed with carbaril insecticide. Treatments consisted of 1,4-dimethoxybenzene (one or two dispensers per trap, 1,4-dimethoxybenze + indole, 1,4-dimethoxybenzene + cinnameldehyde and control. Volatile average release rates (over ten days was approximately 32 mg day-1 per dispenser under laboratory conditions. 1,4-dimethoxybenzene-lured traps caught significantly more beetles than the control, three and seven days after trap setting. Ten days after the onset of the experiment, there were no differences in number of beetles caught by treatments. Captures were higher in the 1,4-dimethoxybenzene + cinnamaldehyde treatment than in 1,4-dimethoxybenzene only in the first assessment. Adding indole to 1,4-dimethoxybenzene did not improve beetle captures.O besouro Astylus variegatus (Germ. (Coleoptera: Melyridae é freqüentemente encontrado em flores onde se alimenta de pólen. Respostas de A. variegatus a atraentes voláteis florais foram estudadas em campos de feijão, Phaseolus vulgaris L. Armadilhas, originalmente desenvolvidas para capturar Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, consistiram de garrafas plástica (2 L com 150 perfurações (5 mm de diâmetro pintadas com tinta amarelo ouro contendo no seu interior uma fita plástica (3,5 ´ 25,0 cm com pó seco de frutos de Lagenaria vulgaris (L. (0,28% de cucurbitacina B estimulante alimentar e arrestante para diabrotic

  12. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae. A atividade inseticida de Toona sureni (Blume Merr. foi avaliada considerando repelência, mortalidade e a produção de progênie de adultos F1 de Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Extrato seco de sementes

  13. A diversidade inventarial de Coleoptera (Insecta em uma paisagem antropizada do Bioma Araucária The inventory diversity of Coleoptera (Insecta of an anthropized landscape in the Biome Araucaria

    Directory of Open Access Journals (Sweden)

    Norma G. Ganho

    2005-12-01

    Full Text Available Como parte do Projeto PROVIVE, foram analisadas a riqueza de espécies, a composição taxonômica, a proporção de espécies raras e a constância taxonômica ao nível de família relacionada à riqueza de espécies, em comunidades de Coleoptera, em Vila Velha, Ponta Grossa, Paraná. Os dados foram obtidos a partir de coletas através de armadilha malaise no estrato do sub-bosque de cinco áreas com diferentes graus de intervenção antrópica, de setembro de 1999 a agosto de 2000. As 52 semanas de amostragem nas cinco áreas resultaram na coleta de 10.822 indivíduos de 1659 espécies. Todas as áreas apresentaram alta riqueza de espécies e diversidade, como indicado por vários índices. A área em estágio mais avançado de sucessão vegetal foi menos rica do que aquelas em estágio inicial/intermediária. De acordo com diferentes estimadores de riqueza de espécies, o número de espécies coletadas poderia aumentar de 22-123% com o aumento do esforço de coleta. As áreas menos conservadas foram mais ricas em espécies raras ("singletons", "doubletons" e únicas do que as mais conservadas. Nas cinco áreas houve uma constância taxonômica entre as famílias mais ricas (Curculionidae, Chrysomelidae, Cerambycidae, Staphylinidae, Mordelidae, Elateridae, Scarabaeidae, Coccinellidae e Tenebrionidae envolvendo 60% do total de espécies, como observado para a abundância de indivíduos. A existência de um padrão de constância taxonômica de famílias, quando considerados 60% da riqueza de espécies e/ou de abundância de indivíduos por local, poderá tornar mais fácil e rápido o estudo de comunidades de Coleoptera, habilitando a ordem a ser um táxon indicador de condições ambientais de áreas florestadas.The species richness, taxonomic composition, rare species, and taxonomic constancy at family level were studied in communities of Coleoptera in Vila Velha, Ponta Grossa, Paraná, as part of PROVIVE project. The data were gathered

  14. A scientific note on a new pest for European honeybees: first report of small hive beetle Aethina tumida, (Coleoptera: Nitidulidae) in Italy

    OpenAIRE

    Palmeri, Vincenzo; Scirtò, Giuseppe; Malacrinò, Antonino; Laudani, Francesca; Campolo, Orlando

    2015-01-01

    International audience The small hive beetle (SHB), Aethina tumida Murray(Coleoptera Nitidulidae), is an important pest of honeybeesin many parts of the world. The beetle is native tosub-Saharan Africa and was unintentionally introducedinto USA (1996), Australia (2001) and in Canada(2002) (Ellis and Munn, 2005; Neumann and Ellis2008). In Europe the SHB was discovered in 2005 ina consignment of queens imported from Texas (USA)into Portugal and destroyed by the Portuguese NationalVeterinaria...

  15. Plant compounds insecticide activity against Coleoptera pests of stored products Compostos de plantas com atividade inseticida a coleópteros-praga de produtos armazenados

    OpenAIRE

    Márcio Dionizio Moreira; Marcelo Coutinho Picanço; Luiz Cláudio de Almeida Barbosa; Raul Narciso Carvalho Guedes; Mateus Ribeiro de Campos; Gerson Adriano Silva; Júlio Cláudio Martins

    2007-01-01

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed...

  16. Mortality and impaired development of Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae) induced by extract of sangra d’água Croton urucurana Baill (Euphorbiaceae).

    OpenAIRE

    Gabriel dos Santos Carvalho; Leonardo Santana da Silva; Luciana Barboza Silva; Mayra Layra dos Santos Almeida; Bruno Ettore Pavam; Marize Terezinha Lopes Pereira Peres

    2014-01-01

    Phaseolus vulgaris L., the green bean, is an important source of protein and minerals in the diet of the population. But its productivity is affected by the attack of numerous pests, from emergence to storage, Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae) being the major pest of stored beans. In the search for alternatives to chemical control of this insect and considering the potential biological activity of plant secondary metabolites, this study was conducted in order to evaluate...

  17. A Temperature-Dependent Development Model for Willow Beetle Species (Coleoptera: Chrysomelidae) in Ireland: Simulation of Phenology/Voltinism in Response to Climate Change

    OpenAIRE

    Pollard, Ciaran P.

    2015-01-01

    Rising fossil fuel prices, energy security and adherence to existing European Union (EU) climate/energy policies means that Ireland must look towards alternative energy sources to meet future demand. Woody biomass in the form of short rotation coppice willow (SRCW) is considered a viable option. SRCW is vulnerable to damage by a range of diseases and pests however. The blue (Phratora vulgatissima) and brown (Galerucella lineola) willow beetles (Coleoptera: Chrysomelidae) are identified econom...

  18. Hexamermis paranaense new species (Nematoda, Mermithidae: a parasite of Diloboderus abderus (Coleoptera, Scarabaeidae in Argentina Hexamermis paranaense sp. nov. (Nematoda, Mermithidae: parasito de Diloboderus abderus (Coleoptera, Scarabaeidae en Argentina

    Directory of Open Access Journals (Sweden)

    María Fernanda Achinelly

    2008-12-01

    Full Text Available A new species, Hexamermis paranaense n. sp. (Nematoda, Mermithidae, a parasite of larvae of Diloboderus abderus Sturm, 1826 (Coleoptera, Scarabaeidae is described and illustrated. This new species is characterized by amphids small, amphidial opening pocket-shaped, the anterior portion of the vagina muscularized and slightly protruding with a descending branch forming a loop before joining the uterus, and three rows of genital papillae: the ventrolateral divided in two rows with eight papillae in the outer row and with six papillae in the inner one; the ventral row with four pairs and one single preanal papillae, and with two pairs, a triplete, one pair, a single and one pair postanal papillae.Una nueva especie, Hexamermis paranaense sp. nov. (Nematoda, Mermithidae, parásito de larvas del gusano blanco, Diloboderus abderus Sturm, 1826 (Coleoptera, Scarabaeidae, se describe e ilustra. Esta nueva especie se caracteriza por poseer anfidos pequeños, con la apertura en forma de bolsillo, parte anterior de la vagina muscular, con una rama que desciende y forma un lazo antes de la conexión al útero, tres hileras de papilas genitales: la hilera de posición ventro-lateral dividida en dos hileras con ocho papilas en la hilera externa y con seis papilas en la interna; la hilera ventral con una papila, seguida de cuatro pares, todas en posición preanal y dos pares, interrumpidos por tres papilas, seguida de un par, una simple, y un par, todas de posición postanal.

  19. Biological control agent of larger black flour beetles (Coleoptera: Tenebrionidae): a nuisance pest developing in cotton gin trash piles.

    Science.gov (United States)

    Nansen, Christian; Stokes, Bryan; James, Jacob; Porter, Patrick; Shields, Eilson J; Wheeler, Terry; Meikle, William G

    2013-04-01

    The larger black flour beetles, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), feeds on saprophytic fungi found in gin trash piles and occasionally becomes a nuisance pest in adjacent homes and businesses. The potential of Steinernema carpocapsae 'NY 001,' as a potential control agent of larger black flour beetle under experimental conditions was examined with particular reference to the importance of soil moisture content. Without prospects of insecticides being labeled for control of larger black flour beetle in gin trash, the data presented here support further research into applications of entomopathogenic nematodes underneath gin trash piles as a way to minimize risk of larger black flour beetle populations causing nuisance to nearby homes and businesses.

  20. How old are the rove beetles (Insecta: Coleoptera: Staphylinidae) and their lineages? Seeking an answer with DNA.

    Science.gov (United States)

    Zhang, Xi; Zhou, Hong-Zhang

    2013-06-01

    The phylogeny and related evolutionary history of rove beetles (Coleoptera, Staphylinidae) remain unclear. This study provides phylogenetic analyses for the family based on three genes (mitochondrial COI, nuclear protein-coding wingless and a portion of the ribosomal 28S rDNA) including 2413 bp for 104 taxa representing most major staphylinid lineages. The subfamilies Oxyporinae, Paederinae, Steninae, and Proteininae are all well-supported clades, as evidenced by all three inference methods, namely maximum parsimony, Bayesian inference, and maximum likelihood. From fossils available for calibration, the divergence time of the main lineages in the family is estimated based on an uncorrelated lognormal relaxed molecular clock analysis method. The molecular clock analysis suggests that the family Staphylinidae dates from approximately the Early Triassic epoch and the most lineages of the family started to radiate from the Late Jurassic to the Early Paleogene.

  1. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    Science.gov (United States)

    Hönnicke, M. G.; Cusatis, C.; Rigon, L.; Menk, R.-H.; Arfelli, F.; Foerster, L. A.; Rosado-Neto, G. H.

    2010-08-01

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures ( Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  2. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    Energy Technology Data Exchange (ETDEWEB)

    Hoennicke, M.G., E-mail: mhonnicke@bnl.go [NSLS II, Brookhaven National Laboratory, Upton, NY (United States); Cusatis, C. [LORXI, Departamento de Fisica-UFPR, Curitiba (Brazil); Rigon, L. [Instituto Nazionale di Fisica Nucleare, Trieste (Italy); Menk, R.-H. [Sincrotrone Trieste SCPa, Basovizza, Trieste (Italy); Arfelli, F. [Instituto Nazionale di Fisica Nucleare, Trieste (Italy); Dipartamento di Fisica-Universita di Trieste, Trieste (Italy); Foerster, L.A.; Rosado-Neto, G.H. [Departamento de Zoologia-UFPR, Curitiba (Brazil)

    2010-08-21

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures (Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  3. The effects of temperature, diet, and other factors on development, survivorship, and oviposition of Aethina tumida (Coleoptera: Nitidulidae).

    Science.gov (United States)

    Meikle, William G; Patt, Joseph M

    2011-06-01

    Developmental rate and survivorship of small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), life stages were measured across different temperatures (21, 25, 28, 32 and 35 degrees C) and diets, which included natural and artificial pollen, honey, and bee pupae. Temperature affected hatch success, time to hatching, and larval growth. Eggs hatched in 61 h at 21 degrees C but in Beetles lived longer at 28 degrees C or lower but produced the most eggs per female, regardless of diet, at 32 degrees C. Beetle density influenced fecundity: beetles kept at three pairs per vial laid 6.7 times more eggs per female than those kept as single pairs. Overall, beetles fared best at 28-32 degrees C with mortality of all stages highest at 35 degrees C. PMID:21735891

  4. Biological control agent of larger black flour beetles (Coleoptera: Tenebrionidae): a nuisance pest developing in cotton gin trash piles.

    Science.gov (United States)

    Nansen, Christian; Stokes, Bryan; James, Jacob; Porter, Patrick; Shields, Eilson J; Wheeler, Terry; Meikle, William G

    2013-04-01

    The larger black flour beetles, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), feeds on saprophytic fungi found in gin trash piles and occasionally becomes a nuisance pest in adjacent homes and businesses. The potential of Steinernema carpocapsae 'NY 001,' as a potential control agent of larger black flour beetle under experimental conditions was examined with particular reference to the importance of soil moisture content. Without prospects of insecticides being labeled for control of larger black flour beetle in gin trash, the data presented here support further research into applications of entomopathogenic nematodes underneath gin trash piles as a way to minimize risk of larger black flour beetle populations causing nuisance to nearby homes and businesses. PMID:23786050

  5. Descriptions of the Larvae of Two Species of Paranomala and One Species of Strigoderma (Coleoptera: Melolonthidae: Rutelinae from Puebla, Mexico

    Directory of Open Access Journals (Sweden)

    Víctor Alfonso Cuate Mozo

    2014-02-01

    Full Text Available  The third instar larvae of Paranomala flavilla  (Bates, P. hoepfneri  (Bates and Strigoderma costulipennis  Bates, collected in Puebla, Mexico, in soils cultivated with amaranth are described. Illustrations of the diagnostic structures and comments on the differences with other larvae of the genera studied in Mexico are included.CARACTERIZACIÓN DE LAS LARVAS DE DOS ESPECIES DE Paranomala Y UNA ESPECIE DE Strigoderma (COLEOPTERA: MELOLONTHIDAE: RUTELINAE DE PUEBLA, MÉXICO.Se presentan las descripciones de la larva de tercer estadio de Paranomala flavilla (Bates, P. hoepfneri (Bates y Strigoderma costulipennis Bates, recolectadas en el estado de Puebla, México, en suelos con cultivo de amaranto (Amaranthus hypochondriacus L.. Se incluyen ilustraciones distintivas y comentarios sobre las diferencias con las larvas de otras especies de los géneros estudiados en México.

  6. Effect of Plant Characteristics and Within-Plant Distribution of Prey on Colonization Efficiency of Cryptolaemus montrouzieri (Coleoptera: Coccinellidae Adults

    Directory of Open Access Journals (Sweden)

    Folukemi Adedipe

    2012-01-01

    Full Text Available Cryptolaemus montrouzieri (Coleoptera: Coccinellidae has been widely used in classical and inundative biological control of mealybugs, including the long-tailed mealybug, Pseudococcus longispinus (Hemiptera: Pseudococcidae. This study was conducted to investigate colonization and establishment efficiency of C. montrouzieri to manage P. longispinus on three different ornamental plant species (Ficus elastica, Lilium longiflorum, and Dieffenbachia seguine. Within-plant distribution pattern of P. longispinus and the colonization ecology of adult C. montrouzieri were investigated. Significantly more P. longispinus were found on the upper parts of the plants regardless of plant species, and C. montrouzieri adults discovered P. longispinus significantly faster when they were released on the top of the plants than on the bottom. Choice tests revealed that C. montrouzieri adults preferred smaller P. longispinus nymphs. The implications for utilization of C. montrouzieri for biological control of mealybugs on various ornamental plants are discussed.

  7. Potential of Tyrophagus putrescentiae (Schrank (Astigmata: Acaridae for the Biological Control of Lasioderma serricorne (F. (Coleoptera: Anobiidae

    Directory of Open Access Journals (Sweden)

    Glauco da Cruz Canevari

    2012-04-01

    Full Text Available This work investigated the potential of Tyrophagus putrescentiae (Schrank (Astigmata: Acaridae to control Lasioderma serricorne (F. (Coleoptera: Anobiidae in the laboratory. L. serricorne is the most destructive insect pest ever found on stored tobacco. The experimental delineation had 33 arenas being each experimental unit with 11 arenas of experiments with eggs, larva of L. serricorne with the density of T. putrescentiae maintained constant, where from 11 of these arenas, eight were for the analysis of predation and three to evaluate the natural mortality of the insect. The highest predation rate was found during the larval stage with, approximately, 54, 68 and 78% mortality of L. serricorne from the fourth until the sixty day of predation. These results indicated that it was possible to use the predatory mite T. putrescentiae in pest management programs of L. serricorne in the storage units of tobacco.

  8. Acoustic detection of Oryctes rhinoceros (Coleoptera: Scarabaeidae: Dynastinae) and Nasutitermes luzonicus (Isoptera: Termitidae) in palm trees in urban Guam.

    Science.gov (United States)

    Mankin, R W; Moore, A

    2010-08-01

    Adult and larval Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae: Dynastinae) were acoustically detected in live and dead palm trees and logs in recently invaded areas of Guam, along with Nasutitermes luzonicus Oshima (Isoptera: Termitidae), and other small, sound-producing invertebrates and invertebrates. The low-frequency, long-duration sound-impulse trains produced by large, active O. rhinoceros and the higher frequency, shorter impulse trains produced by feeding N. luzonicus had distinctive spectral and temporal patterns that facilitated their identification and discrimination from background noise, as well as from roaches, earwigs, and other small sound-producing organisms present in the trees and logs. The distinctiveness of the O. rhinoceros sounds enables current usage of acoustic detection as a tactic in Guam's ongoing O. rhinoceros eradication program.

  9. Predation potential of Chilocorus cacti (Coleoptera: Coccinellidae) to the prickly pear cacti pest Dactylopius opuntiae (Hemiptera: Dactylopiidae).

    Science.gov (United States)

    Flores, A; Olvera, H; Rodríguez, S; Barranco, J

    2013-08-01

    Functional response of the predator Chilocorus cacti (Linnaeus) (Coleoptera: Coccinellidae) on five densities of Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae) female adults was assessed under laboratory conditions. The searching efficiency of C. cacti significantly decreased as prey density increased. The logistic regression for the predator had a negative and significant linear parameter indicating a type II functional response. Non-linear regression for Holling predator equation estimated a handling time of 1.79 ± 0.129 h and attack rate coefficient of 0.1003 ± 0.030. Most of this handling time was because the predator spent a lot of time removing the waxy coating that protects adult females of D. opuntiae. Chilocorus cacti consumes females of D. opuntiae in their reproductive stage; therefore, it could be an effective natural enemy to suppress or regulate low density populations of D. opuntiae, preventing them to reach high densities. PMID:23949861

  10. Genetic differentiation among populations of the beetle Bolitophagus reticulatus (Coleoptera: tenebrionidae) in a fragmented and a continuous landscape.

    Science.gov (United States)

    Knutsen, H; Rukke, B A; Jorde, P E; Ims, R A

    2000-06-01

    The effect of habitat fragmentation on genetic differentiation among local populations of the fungivorous beetle Bolitophagus reticulatus (Coleoptera: Tenebrionidae) was studied in two contrasting landscapes: one heavily fragmented with forest fragments of variable size surrounded by inhabitable agricultural fields, the other an old forest providing a continuous habitat. The genetic structure of the beetle within each of the two contrasting areas was investigated by means of protein electrophoresis, screening four polymorphic loci in 20 populations from each area. In both areas there were significant genetic differences among local populations, but on average differentiation in the fragmented area was three times greater than in the continuous one, strongly indicating a genetic isolation effect of habitat fragmentation. These genetic results are in accordance with previous studies on dispersal in this species. PMID:10886382

  11. Characterization of the proteases in the midgut of the xylophagous larvae of Oemona hirta (Coleoptera:Cerambycidae)

    Institute of Scientific and Technical Information of China (English)

    Brian David Shaw; John Tane Christeller

    2009-01-01

    The protein digestive capability oftbe larvae of the longhorn beetle (Oemona hirta,Coleoptera:Cerambycidae,Fabricius,1775) was investigated.This species feeds only on wood where there is a high proportion of vascular tissue.The pH of the midgut,the major digestive organ,was alkaline and protein hydrolysis was maximal at alkaline pH.Use of specific synthetic peptide substrates showed that the major protease activities were the endopeptidases,trypsin and chymotrypsin-like activity,and the exopeptidase,leucine aminopeptidase and the pH curves corresponded to that with protein substrate.Studies using a range ofsefine protease inhibitors as well as specific inhibitors ofmetalloproteases,cysteine proteases and aspartate proteases confirmed a serine protease-based digestive system similar to earlier reports of sapwood-feeding Cerambycids.Control of these insect pests using protease inhibitors is discussed.

  12. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    International Nuclear Information System (INIS)

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures (Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  13. Molecular evidence of facultative intraguild predation by Monochamus titillator larvae (Coleoptera: Cerambycidae) on members of the southern pine beetle guild

    Science.gov (United States)

    Schoeller, Erich N.; Husseneder, Claudia; Allison, Jeremy D.

    2012-11-01

    The southern pine bark beetle guild (SPBG) is arguably the most destructive group of forest insects in the southeastern USA. This guild contains five species of bark beetles (Coleoptera: Curculionidae: Scolytinae): Dendroctonus frontalis, Dendroctonus terebrans, Ips avulsus, Ips calligraphus, and Ips grandicollis. A diverse community of illicit receivers is attracted to pheromones emitted by the SPBG, including the woodborers Monochamus carolinensis and Monochamus titillator (Coleoptera: Cerambycidae). These woodborers have been traditionally classified as resource competitors; however, laboratory assays suggest that larval M. carolinensis may be facultative intraguild predators of SPBG larvae. This study used polymerase chain reaction (PCR)-based molecular gut content analyses to characterize subcortical interactions between M. titillator and members of the SPBG. The half-lives of SPBG DNA were estimated in the laboratory prior to examining these interactions in the field. A total of 271 field-collected M. titillator larvae were analyzed and 26 (9.6 %) tested positive for DNA of members of the SPBG. Of these larvae, 25 (96.2 %) tested positive for I. grandicollis and one (3.8 %) for I. calligraphus. Failure to detect D. terebrans and D. frontalis was likely due to their absence in the field. I. avulsus was present, but primers developed using adult tissues failed to amplify larval tissue. Results from this study support the hypothesis that larval Monochamus spp. are facultative intraguild predators of bark beetle larvae. Additionally, this study demonstrates the capabilities of PCR in elucidating the interactions of cryptic forest insects and provides a tool to better understand mechanisms driving southern pine beetle guild population fluctuations.

  14. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.

  15. Intra-Annual Variation in Responses by Flying Southern Pine Beetles (Coleoptera: Curculionidae: Scolytinae) to Pheromone Component endo-Brevicomin.

    Science.gov (United States)

    Sullivan, Brian T; Brownie, Cavell; Barrett, JoAnne P

    2016-08-01

    The southern pine beetle Dendroctonus frontalis Zimmermann (Coleoptera: Curculionidae: Scolytinae) is attracted to an aggregation pheromone that includes the multifunctional pheromone component endo-brevicomin. The effect of endo-brevicomin on attractive lures varies from strong enhancement to reduction of beetle attraction depending upon release rate, lure component spacing, and proximity of beetle infestations. Anecdotal observations have further suggested that the effects of endo-brevicomin vary during the year. We investigated this possibility under nonoutbreak conditions in southwestern Mississippi where for two-and-a-half years we monitored traps baited with frontalin and the host odor alpha-pinene either (a) alone, or with an endo-brevicomin release device either (b) located directly on the trap, or (c) displaced 6 m away. The endo-brevicomin devices in our tests increased D. frontalis catches during all times of year, and 6 m displacement of the endo-brevicomin release device from the trap did not significantly alter responses except during the spring flight peak when displacement increased catches. Our data suggest that flying D. frontalis have a stronger tendency to avoid the immediate proximity of a release point of endo-brevicomin during their springtime dispersal flight when catches are greatest. Catches of Thanasimus dubius (F.) (Coleoptera: Cleridae), a major predator of D. frontalis, were not altered by endo-brevicomin, and ratios of D. frontalis to T. dubius changed over the course of the year. We discuss the possible effects of intra-annual variation in D. frontalis response to endo-brevicomin both on beetle attack behavior and use of endo-brevicomin as a lure adjuvant in D. frontalis population monitoring.

  16. Parasitylenchus bifurcatus n. sp. (Tylenchida: Allantonematidae parasitizing Harmonia axyridis (Coleoptera: Coccinellidae

    Directory of Open Access Journals (Sweden)

    Poinar George O

    2012-10-01

    Full Text Available Abstract Background The harlequin ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae is native to central and eastern Asia and was purposely introduced into Europe to control aphids. While it proved to be a good biological control agent, its rapid spread and buildup of large populations made it a nuisance, since it overwinters in homes, emits unpleasant odors, stains fabrics, occasionally bites humans and feeds on apples, pears and grapes. Aside from the above, the ravenous appetite of H. axyridis results in their consumption of harmless native insects, including even other ladybird beetles. A study of the natural enemies of H. axyridis in Denmark revealed the presence of nematodes. The present study describes this nematode parasite and discusses aspects of its development and ecology. Methods Adult harlequin ladybird beetles were collected from March to November from four localities in Copenhagen on different plant species. In addition, groups of last-instar larvae and pupae (n = 50 were examined for the presence of nematodes. Living and recently dead nematodes were removed from adult H. axyridis in 0.5% saline solution, the nematodes were then heat killed (at 75C, fixed in 5% formalin and transferred to glycerin on slides for further examination and measurements. Results A new species of Allantonematidae (Tylenchida, Parasitylenchus bifurcatus n. sp., is described from adults of the harlequin ladybird, Harmonia axyridis in Denmark. The new species is characterized by a straight stylet lacking basal thickenings, a bursa and a forked tail tip in the vermiform (infective females and juvenile males. The new species is compared with P. coccinellinae previously described from ladybird beetles in France. Parasitism resulted in depletion of the fat body and partial or complete atrophy of the reproductive organs of the beetles. Infections occurred throughout the year with rates of parasitism reaching up to 35%. The rate increased to 60

  17. Diversity and altitudinal distribution of Chrysomelidae (Coleoptera in Peregrina Canyon, Tamaulipas, Mexico

    Directory of Open Access Journals (Sweden)

    Uriel Jeshua Sánchez-Reyes

    2014-06-01

    Full Text Available The Chrysomelidae (Coleoptera is a highly speciose family that has been poorly studied at the regional level in Mexico. In the present study, we estimated species richness and diversity in oak-pine forest, Tamaulipan thorny scrub and in tropical deciduous forests in Peregrina Canyon within the Altas Cumbres Protected Area of the northeastern state of Tamaulipas, Mexico. Sampling of Chrysomelidae consisted of five sweep net samples (200 net sweeps within each of three sites during four sample periods: early dry season, late dry season, early wet season, and late wet season. Species were identified and total numbers per species were recorded for each sample. A total of 2,226 specimens were collected belonging to six subfamilies, 81 genera and 157 species of Chrysomelidae from the study area. Galerucinae was the most abundant subfamily with 1,828 specimens, representing 82.1% of total abundance in the study area. Lower abundance was recorded in Cassidinae (8.5%, Eumolpinae (3.6%, Cryptocephalinae (2.2%, Chrysomelinae (2.2%, and finally Criocerinae (1.3%. The highest species richness was also presented in the subfamily Galerucinae with 49% of the total obtained species followed by Cassidinae (20%, Cryptocephalinae (9.7%, Eumolpinae (9.7%, Chrysomelinae (6.5% and Criocerinae (5.2%. The most common species were Centralaphthona fulvipennis Jacoby (412 individuals, Centralaphthona diversa (Baly (248, Margaridisa sp.1 (219, Acallepitrix sp.1 (134, Longitarsus sp.1 (104, Heterispa vinula (Erichson (91, Epitrix sp.1 (84 and Chaetocnema sp.1 (72. Twenty-two species were doubletons (1.97% of total abundance and 52 were singletons (2.33%. The estimated overall density value obtained was 0.0037 individuals/m2. The greatest abundance and density of individuals were recorded at the lowest elevation site. However, alpha diversity increased with increasing altitude. Similarity values were less than 50% among the three sites indicating that each site had distinct

  18. 从野生与突变株云芝IBL-04提纯锰过氧化物酶及其表征%Purification and characterization of manganese peroxidases from native and mutant Trametes versicolor IBL-04

    Institute of Scientific and Technical Information of China (English)

    Muhammad Asgher; Muhammad Ramzan; Muhammad Bilal

    2016-01-01

    Extracellular manganese peroxidases (MnPs) produced by native and mutant strains of Trametes versicolor IBL‐04 (EB‐60, EMS‐90) were purified by ammonium sulphate precipitation and dialysis, followed by ion‐exchange and gel‐permeation chromatography. The purified enzymes elucidated a single band in the 43‐kDa region on sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. The optimum pH and temperature of the purified enzymes were found to be 5.0 and 40 °C, respec‐tively. Mutant strain MnPs exhibited a broader active pH range and higher thermal stability than native MnP. Purified MnPs from selected mutants showed almost identical properties to native MnP in electrophoresis, steady‐state kinetics, and metal ion and endocrine‐disrupting compound (EDC) degradation efficiency. Although the fastest reaction rates occurred with Mn2+, MnPs displayed the highest affinity for ABTS, methoxyhydroquinone, 4‐aminophenol and reactive dyes. MnP activity was significantly enhanced by Mn2+and Cu2+, and inhibited in the presence of Zn2+, Fe2+, ethylene‐diaminetetraacetic acid and cysteine to various extents, with Hg2+ as the most potent inhibitory agent. MnPs from all sources efficiently catalyzed the degradation of the EDCs, nonylphenol and triclosan, removing over 80%after 3 h of treatment, which was further increased up to 90%in the presence of MnP‐mediator system. The properties of T. versicolor MnPs, such as high pH and ther‐mal stability, as well as unique Michaelis‐Menten kinetic parameters and high EDC elimination effi‐ciency, render them promising candidates for industrial exploitation.%由野生及突变株云芝IBL-04制得细胞外锰过氧化物酶(MnPs),并经过硫酸铵沉淀、透析、离子交换和凝胶渗透层析法等步骤提纯.纯化的酶在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)上于43 kDa区域呈现单一谱带,它适宜的pH值和温度分别为5.0和40°C.突变株MnPs表现出比野生

  19. Oryctini (Coleoptera: Scarabaeidae: Dynastinae da coleção de invertebrados do Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas, Brasil Oryctini (Coleoptera: Scarabaeidae: Dynastinae of the invertebrate collection of the National Institute of Research of Amazonia (INPA, Manaus, Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Hector Jaime Gasca

    2009-09-01

    Full Text Available A Coleção de Invertebrados do Instituto Nacional de Pesquisas da Amazônia (INPA possui 554 espécimes de Oryctini (Coleoptera: Scarabaeidae: Dynastinae, pertencentes a 7 gêneros, 16 espécies e 2 subespécies. As distribuições geográficas das espécies são fornecidas, sendo que 97% do material examinado procedem de coletas feitas em diferentes locais da Amazônia brasileira.The Invertebrate Collection of the Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas, Brazil, has 554 specimens of Oryctini (Coleoptera: Scarabaeidae: Dynastinae, belonging to seven genera, 16 species and two subspecies. Information about geographical distribution are provided, of which 97% of the material examined was collected from several places in the Brazilian Amazon.

  20. Fauna de Coleoptera no Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brasil: abundância e riqueza das famílias capturadas através de armadilhas de solo Coleoptera fauna in the Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brazil: abundance and family richness captured with pitfall traps

    OpenAIRE

    Renato C. Marinoni; Norma G. Ganho

    2003-01-01

    This paper is a part of the studies on the Coleoptera fauna from Vila Velha, Ponta Grossa, Parana gathered through malaise and pitfall traps in sites with different floristic conditions. The present study deal with the data of pitfall trap captures, installed close to the malaise traps. The data were obtained weekly (52 samples), from September 1999 to August 2000. This survey was carried out on five areas, three of them with different plant succession stages (initial, intermediate, and advan...