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Sample records for apagomerella versicolor coleoptera

  1. Tinea versicolor

    Science.gov (United States)

    ... JavaScript. Tinea versicolor is a long-term (chronic) fungal infection of the skin . Causes Tinea versicolor is fairly ... back References Hay RJ. Dermatophytosis and other superficial mycoses. In: Mandell GL, Bennett JE, Dolin R, eds. ...

  2. Tinea Versicolor

    Science.gov (United States)

    ... resources Meet our partners Español Donate Diseases and treatments Acne and rosacea Bumps and growths Color problems Contagious skin diseases ... Tinea versicolor public SPOT Skin Cancer™ Diseases and treatments Acne and rosacea Bumps and growths Color problems Acanthosis nigricans Melasma ...

  3. Malassezia Species and Pityriasis Versicolor

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    Gulin Rodoplu

    2016-01-01

    Full Text Available Malassezia species are found in part of the normal human cutaneous commensal flora, however it has been known for many years that the Malassezia yeasts are associated with a number of different human diseases ranging from pityriasis versicolor to seborrhoeic dermatitis. In addition, since the 1980s, they have been reported as causing opportunistic systemic infections. The taxonomy of Malassezia spp. has recently been modified to include 13 obligatorily lipophilic species, plus one non-obligatorily lipophilic species, which only rarely colonizes human hosts and currently the genus consist 14 species as M. furfur, M. pachydermatis, M. sympodialis, M. globosa, M. obtusa, M. slooffiae, M. restricta, M. dermatis, M. japonica, M. nana, M. yamatoensis, M. caprae, M. equina, M. cuniculi. Fastidious growth requirements of Malassezia yeasts defied the initial attempts to culture these organisms and their true identification and the relationship between different species only became apparent with the application of modern molecular techniques. The causative fungus is seen especially in such seborrheic areas as the scalp, face, trunk and upper back. Under the influence of various exogenous or endogenous predisposing factors, these yeasts change from the blastospore form to the mycelial form and become pathogenic. Diagnosis of pityriasis versicolor which is caused by Malassezia species is generally easy and lies on the basis of its clinical appearance and can be confirmed by mycological examination. The diagnosisis is mainly based on direct examination with potassium hydroxide (KOH and demonstration that represents pseudohyphae and blastoconidia as the typical %u201Cspaghetti and meatballs%u201D pattern. Characteristic features of the genus Malassezia include a distinctive morphology and an affinity for lipids in culture. Culture is necessary to recover the infecting strain, especially for epidemiologic purposes and also to test its antifungal susceptibility

  4. A Steroidal Glycoside from Cynanchum versicolor Bunge

    Institute of Scientific and Technical Information of China (English)

    Zhao Guang ZHENG; Run Hui LIU; Ling Yi KONG; Wei Dong ZHANG

    2006-01-01

    A new C21 steroidal glycoside, named cynanversicoside F (1), was isolated from the root of Cynanchum versicolor Bunge. Its structure was established as glaucogenin-A 3-O-β-D-digitoxopyranosyl-(1→4)-β-D-cymaropyranoside by spectroscopic and chemical methods.

  5. CULTIVATION OF TRAMETES VERSICOLOR IN MEXICO

    OpenAIRE

    D. González Guerrero; V. Esparza Martínez; R. de la Torre Almaráz

    2011-01-01

    A native strain of Trametes versicolor (Coriolaceae) was isolated and cultivated under laboratory conditions. Mycelial colonies were off-white, showing high density, velvety texture, and abundant aerial hyphae. Substrates studied had good mycelial growth and colonization. Higher mushroom yield of 173.8 g was recorded on supplemented oak sawdust, reaching a biological efficiency of 20.3%. Leathery, dark brown fruit bodies were obtained having normal morphology. A lower biological efficiency of...

  6. Aflatoxin Control in Maize by Trametes versicolor

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    Marzia Scarpari

    2014-12-01

    Full Text Available Aspergillus flavus is a well-known ubiquitous fungus able to contaminate both in pre- and postharvest period different feed and food commodities. During their growth, these fungi can synthesise aflatoxins, secondary metabolites highly hazardous for animal and human health. The requirement of products with low impact on the environment and on human health, able to control aflatoxin production, has increased. In this work the effect of the basidiomycete Trametes versicolor on the aflatoxin production by A. flavus both in vitro and in maize, was investigated. The goal was to propose an environmental loyal tool for a significant control of aflatoxin production, in order to obtain feedstuffs and feed with a high standard of quality and safety to enhance the wellbeing of dairy cows. The presence of T. versicolor, grown on sugar beet pulp, inhibited the production of aflatoxin B1 in maize by A. flavus. Furthermore, treatment of contaminated maize with culture filtrates of T. versicolor containing ligninolytic enzymes, showed a significant reduction of the content of aflatoxin B1.

  7. Transformation of Triclosan by Trametes versicolor and Pycnoporus cinnabarinus

    OpenAIRE

    Hundt, Kai; Martin, Dierk; Hammer, Elke; Jonas, Ulrike; Kindermann, Markus Karl; Schauer, Frieder

    2000-01-01

    We investigated the ability of Trametes versicolor and Pycnoporous cinnabarinus to metabolize triclosan. T. versicolor produced three metabolites, 2-O-(2,4,4′-trichlorodiphenyl ether)-β-d-xylopyranoside, 2-O-(2,4,4′-trichlorodiphenyl ether)-β-d-glucopyranoside, and 2,4-dichlorophenol. P. cinnabarinus converted triclosan to 2,4,4′-trichloro-2′-methoxydiphenyl ether and the glucoside conjugate known from T. versicolor. The conjugates showed a distinctly lower cytotoxic and microbicidal activity...

  8. Ketoconazole 2% shampoo in pityriasis versicolor: An open trial

    OpenAIRE

    Rathi S

    2003-01-01

    Pityriasis versicolor is a common superficial fungal infection caused by a lipophilic yeast. The aim of the study was to evaluate the efficacy of ketoconazole 2% shampoo in the treatment of pityriasis versicolor, for which thirty patients were included. The shampoo was applied daily for 3 days and found to be very effective in clearing the signs and symptoms of the disease. There was no serious adverse effects.

  9. Ketoconazole 2% shampoo in pityriasis versicolor: An open trial

    Directory of Open Access Journals (Sweden)

    Rathi S

    2003-03-01

    Full Text Available Pityriasis versicolor is a common superficial fungal infection caused by a lipophilic yeast. The aim of the study was to evaluate the efficacy of ketoconazole 2% shampoo in the treatment of pityriasis versicolor, for which thirty patients were included. The shampoo was applied daily for 3 days and found to be very effective in clearing the signs and symptoms of the disease. There was no serious adverse effects.

  10. IN VITRO PROPAGATION OF ALTERNANTHERA VERSICOLOR (LINN.) - A MEDICINAL HERB

    OpenAIRE

    D. Preetha; K. Mahalakshmi; M. Bhakyalakshmi

    2013-01-01

    A complete protocol for micropropagation of Alternanthera versicolor using leaf and internodal explants was developed. An explants from A. versicolor established on Murashige and Skoog medium were treated with various concentrations and combinations of auxins and cytokinins to determine the best method for callus formation, shoot regeneration and root formation. MS medium supplemented with 1 mg/l BAP and 1 mg/l IAA was determined to be the most suitable for callus induction. After 2 weeks, th...

  11. Solubilization of Leonardite by an Extracellular Fraction from Coriolus versicolor

    OpenAIRE

    Pyne, John W.; Stewart, Dorothy L.; Fredrickson, James; Wilson, Bary W.

    1987-01-01

    Coriolus versicolor has previously been shown to degrade leonardite, an oxidized form of lignite. An extracellular fraction containing protein purified from a C. versicolor culture solubilized leonardite in vitro. Expression of the activity did not require the presence of leonardite and appeared during idiophase. During ion-exchange and gel filtration column chromatography, leonardite-biosolubilizing activity eluted with syringaldazine oxidase activity and with protein, as measured by A280 an...

  12. Trametes versicolor Mushroom Immune Therapy in Breast Cancer

    OpenAIRE

    Standish, Leanna J.; WENNER, CYNTHIA A.; Sweet, Erin S.; Bridge, Carly; Nelson, Ana; Martzen, Mark; Novack, Jeffrey; Torkelson, Carolyn

    2008-01-01

    Data from multiple epidemiologic and clinical studies on immune effects of conventional cancer treatment and the clinical benefits of polysaccharide immune therapy suggest that immune function has a role in breast cancer prevention. Immune therapy utilizing the polysaccharide constituents of Trametes versicolor (Tv) as concurrent adjuvant cancer therapy may be warranted as part of a comprehensive cancer treatment and secondary prevention strategy.

  13. The effect of surfactant on pollutant biosorption of Trametes versicolor

    Science.gov (United States)

    Gül, Ülküye Dudu; Silah, Hülya; Akbaş, Halide; Has, Merve

    2016-04-01

    The major problem concerning industrial wastewater is treatment of dye and heavy metal containing effluents. Industrial effluents are also contained surfactants that are used as levelling, dispersing and wetting agents. The purpose of this study was to investigate the effect of surfactant on textile dye biosorption properties of a white rot fungus named Trametes versicolor. Reactive dyes are commonly used in textile industry because of their advantages such as brightness and excellent color fastness. A recative textile dye, called Everzol Black, was used in this study. The low-cost mollasses medium is used for fungal growth. The usage of mollases, the sugar refinery effluent as a source of energy and nutrients, gained importance because of reducing the cost and also reusing another waste. In biosorption process the effect of surfactant on dye removal properties of T. versicolor was examined as a function of pH, dye consentration and surfactant concentration. The results of this study showed that the surfactant enhanced the dye removal capacity of Trametes versicolor. The dye and surfactant molecules were interacted electrostatically and these electrostatic interactions improved dye removal properties of filamentous fungus T. versicolor. The results of this study recommended the use of surfactants as an inducer in textile wastewater treatment technologies.

  14. Trametes versicolor extract modifies human fecal microbiota composition in vitro.

    Science.gov (United States)

    Yu, Zhuo-Teng; Liu, Bo; Mukherjee, Purna; Newburg, David S

    2013-06-01

    Trametes versicolor is a mushroom used as a traditional Chinese medicine (Yun-zhi) for a wide array of seemingly disparate conditions. We hypothesized that many of its multiple purported activities could be mediated through stimulation of beneficial mutualist components of the microbiota. Human fecal microbiota was cultured anaerobically to determine its ability to ferment a common extract of T. versicolor, designated polysaccharide peptide (PSP), and the ability of PSP to alter the composition of the microbial community. The presence of PSP and fructooligosaccharides (FOS, a common prebiotic) in the medium, but not cellulose, significantly increased levels of Bifidobacterium spp. PSP also elevated Lactobacillus spp., while reducing Clostridium spp., Staphylococcus spp. and Enterococcus spp. Levels of Streptococcus spp., Bacteroides spp. and Escherichia did not significantly change. Fermentation of PSP increased the concentration of organic acids (lactate and short-chain fatty acids), decreased the pH, and induced β-galactosidase and β-glucosidase activities. The genera of the human microbiota that are promoted by FOS and other prebiotics are also stimulated by the Trametes versicolor extract, PSP. Thus, Trametes versicolor, a common East Asian botanical, contains putative prebiotic agents that alter human gut microbiota and pH. This prebiotic-like activity may help explain some of the plethora of the health benefits attributed to this traditional Chinese medicine. PMID:23435630

  15. Prevalence of different Malassezia species in pityriasis versicolor in central India

    OpenAIRE

    Chaudhary Rahul; Singh Sanjay; Banerjee Tuhina; Tilak Ragini

    2010-01-01

    Background: In the last 10 years, different studies have shown interesting geographical variations in the prevalence of different Malassezia species in pityriasis versicolor. Aim: Identification of Malassezia species isolated from patients with pityriasis versicolor. Methods: In 100 patients with pityriasis versicolor, Malassezia species were identified by culture in Sabouraud′s dextrose agar containing cycloheximide with olive oil overlay and modified Dixon agar and by doing biochemic...

  16. Four Novel Hydropyranoindeno- Derivatives from Marine Fungus Aspergillus versicolor

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    From the cultured filtrates of fungus Aspergillus versicolor,isolated from marine sponge Xestospongia exigua,four novel secondary metabolites,namely aspergillone 1,aspergillodiol 2, aspergillol 3 and 12-acetyl-aspergillol 4,have been isolated by column chromatographic separation.The structures of all the new compounds are established on the basis of extensive 2D NMR spectroscopy in conjugation with MS,UV spectral analysis.The basic structure pattern of those compounds possessed an hydroindenoisopyran nucleus.

  17. Comparative histopathology of Pityriasis versicolor and Pityrosporum folliculitis

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    Mittal R

    1992-01-01

    Full Text Available Twenty five cases each of pityriasis versicolor (PV and pityrosporum folliculitis (PF were selected for this study. Histopathologically in PV, spores and hyphae in spaghetti and meatball appearance in stratum corneum, and perivascular mononuclear infiltrate were seen. In PF, spores were inside the dilated hair follicles filled with keratin, perifollicular and perivascular mononuclear infiltration were seen. Basket weave hyperkeratosis, edema and disruption of collagen were seen in 88% cases of PF and have not been reported earlier.

  18. [Pityriasis versicolor--modern views on etiology, pathogenesis and therapy].

    Science.gov (United States)

    Karadaglić, Dj

    1992-01-01

    Pityriasis versicolor (Tinea versicolor) is a superficial chronic fungal infection caused by Pityrisporum species which are normal "inhabitants" of the cutaneous flora. The morphologic changes from yeast to mycelial hypha form are important in the development of clinical lesions. The onset and course of the disease are under the influence of genetic factors, age, sex, climate, local environmental factors, malnutrition, pregnancy, oral contraceptives, corticosteroid and immunosuppressive treatment. They favorize transformation of saphrophytic to pathogenic form, and are the cause of recurrences and chronicity of the disease. The ultrastructural and immunologic studies which are carried out today would significantly contribute to a better understanding of pathogenesis of the disease. The disease is limited to seborrheic areas of the skin and commonly has three clinical forms: papulosquamous, follicular and inverse. This can make great problems in differential diagnosis. In the treatment of these patients it should be kept in mind that Pityriasis versicolor is not a primary contagious disease and that conversion of Pityrisporum depends on the predisposing factors. Numerous medicaments with local and systemic effect which are used nowaday in the treatment and prevention of pityriasis are reported. Way of application, doses, duration of therapy, advantages and disadvantages are given in detail. PMID:1465674

  19. Clinical Trial Of Ketoconazole 2% + Zinc Pyrithione 1% Shampoo In The Management Of Tinea Versicolor

    OpenAIRE

    Ramesh M; Bhat Aparna; Shetty Sohandas

    2004-01-01

    An open uncontrolled trial, to assess the efficacy of ketoconazole 2%+zinc pyrithione 1% shampoo was conducted in patients with extensive tinea versicolor. The results show that this is an effective and well tolerated combination in the management of tinea versicolor.

  20. Clinical Trial Of Ketoconazole 2% + Zinc Pyrithione 1% Shampoo In The Management Of Tinea Versicolor

    Directory of Open Access Journals (Sweden)

    Ramesh M

    2004-01-01

    Full Text Available An open uncontrolled trial, to assess the efficacy of ketoconazole 2%+zinc pyrithione 1% shampoo was conducted in patients with extensive tinea versicolor. The results show that this is an effective and well tolerated combination in the management of tinea versicolor.

  1. Adult Diapause in Coleoptera

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    Ivo Hodek

    2012-01-01

    Full Text Available Recent studies dealing with adult (reproductive diapause in the Coleoptera are reviewed, as a kind of supplement to the classic compendia. In the first two sections, the general characteristics of adult diapause are described and principal terms explained. Original articles dealing with 19 species from nine coleopteran families (Coccinellidae, Chrysomelidae, Bruchidae, Curculionidae, Carabidae, Silphidae, Scolytidae, Scarabaeidae, and Endomychidae are reviewed. Finally attempts are made at generalisations from the papers reviewed, and hypotheses on diapause evolution are inferred. A polyphenic character of diapause is a prominent feature in C. septempunctata and L. decemlineata, but has been found also in other Coleoptera and in insects generally and often generates voltinism heterogeneity within populations.

  2. Структура комплексов жужелиц (Insecta: Coleoptera: Carabidae) плантационных посадок брусники в условиях Беларуси в первые годы после закладки

    OpenAIRE

    Буга, С. В.; О.Р. Александрович; Морозов, О. В.

    2013-01-01

    Species composition and ecological structure of carabid beetles (Insecta: Coleoptera: Carabidae) community at cowberry plantations were studied in SW Belarus in the first years after planting. Terrestrial invertebrates were sampled by pitfall traps. A total of 3028 imagines of 45 carabid species have been collected. Activity density of carabid beetles was the highest from late May to early June. Poecilus versicolor (Sturm, 1824) was the most abundant (87,39 %). Species richness of predator and...

  3. Effects of catalysts on liquefaction of Agaricus versicolor (L.)

    Science.gov (United States)

    Durak, Halil

    2016-04-01

    Supercritical liquefaction process is used for producing energy from biomass. The common reaction conditions for supercritical liquefaction process are the 240-380 °C temperature range and 5-20 Mpa pressure values range. Agaricus versicolor (L.) was liquefied by acetone in an autoclave (75 mL) under high pressure with (aluminium oxide and calcium hydroxide) and without catalyst at 290 °C for producing bio-oil. The products of liquefaction (bio-oil) were analysed and characterized using various methods including elemental analysis, Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. GC-MS identified 27 different compounds in the bio-oils obtained at 290 °C.

  4. Структура комплексов жужелиц (Insecta: Coleoptera: Carabidae) плантационных посадок голубики высокорослой в условиях Беларуси в первые годы после закладки

    OpenAIRE

    Буга, С. В.; О.Р. Александрович

    2013-01-01

    Species composition and ecological structure of carabid beetles (Insecta: Coleoptera: Carabidae) communities at highbush blueberry plantations were studied in SW Belarus in the first years after planting. Terrestrial invertebrates were sampled by pitfall traps. A total of 623 imagines of 51 carabid species have been collected. Field and meadow mesophilous and mesokserophilous species are formed main part of whole carabid complex. Poecilus versicolor (Sturm, 1824) and Agonum sexpunctatum (...

  5. Comparing Efficacy Of Itraconazole Versus Itraconazole Plus 2% Ketoconazole Shampoo In The Treatment Of Pityriasis Versicolor: Retrospective Study

    OpenAIRE

    Karıncaoğlu, Yelda; Özcan, Hamdi; Akı, Tuba; Eşrefoğlu, Muammer

    2003-01-01

    Background and Design: Tinea versicolor is a frequent superficial fungal infection caused by a lipophilic yeast; Malessezia furfur. Topical ketoconazole shampoo and systemic itraconazole can be used for the treatment of tinea versicolor. In this study, the efficiency of itraconazole versus itraconazole plus 2% ketoconazole shampoo in the treatment of tinea versicolor is evaluated retrospectively. Materials and Methods: The patients, who were admitted to dermatology department ...

  6. Bioremediation of zirconium from aqueous solution by coriolus versicolor: process optimization

    International Nuclear Information System (INIS)

    In the present study the potential of live mycelia of Coriolus versicolor was explored for the removal of zirconium from simulated aqueous solution. Optimum experimental parameters for the bioremediation of zirconium using C. versicolor biomass have been investigated by studying the effect of mycelia dose, concentration of zirconium, contact time and temperature. The isothermal studies indicated that the ongoing bioremediation process was exothermic in nature and obeyed Langmuir adsorption isotherm model. The Gibbs free energy (ΔG), entropy (ΔS) and enthalpy (ΔH) of bioremediation were also determined. The result showed that bioremediation of zirconium by live C. versicolor was feasible and spontaneous at room temperature. The equilibrium data verified the involvement of chemisorption during the bioremediation. The kinetic data indicated the operation of pseudo-second order process during the biosorption of zirconium from aqueous solution. Maximum bioremediation capacity (110.75 mg/g) of C. versicolor was observed under optimum operational conditions: pH 4.5, biomass dose 0.05 mg/100 mL, contact time 6 h and temperature 30 degree C. The results showed that C. versicolor could be used for bioremediation of heavy metal ions from aqueous systems. (author)

  7. Occurrence of the Toxin-Producing Aspergillus versicolor Tiraboschi in Residential Buildings.

    Science.gov (United States)

    Piontek, Marlena; Łuszczyńska, Katarzyna; Lechów, Hanna

    2016-01-01

    In an area representative of a moderate climate zone (Lubuskie Province in Poland), mycological tests in over 270 flats demonstrated the occurrence of 82 species of moulds. Aspergillus versicolor Tiraboschi was often encountered on building partitions (frequency 4: frequently). The ability to synthesize the carcinogenic sterigmatocystin (ST) means that it poses a risk to humans and animals. Biotoxicological tests of biomasses of A. versicolor were conducted in the Microbiological and Toxicological Laboratory, using the planarians Dugesia tigrina (Girard). The obtained results of the tests covered a broad range of toxicity levels of isolated strains: from weakly toxic (100-1000 mg·L(-3)) to potently toxic (1-10 mg·L(-3)). The high-performance liquid chromatography (HPLC) physicochemical method confirmed the ability of A. versicolor strains to synthesize sterigmatocystin. All of the samples of the air-dry biomasses of the fungi contained ST in the range between 0.03 and 534.38 mg·kg(-1). In the bio-safety level (BSL) classification A. versicolor belongs to category 1. Additionally, A. versicolor is an allergenic mould. PMID:27589778

  8. Pityriasis versicolor: A clinicomycological and epidemiological study from a tertiary care hospital

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    Ghosh Sudip

    2008-01-01

    Full Text Available Background: Pityriasis versicolor is a mild, chronic, usually asymptomatic superficial fungal infection of the stratum corneum, caused by Malassezia yeasts. The purpose of the present study is to assess the clinical profile of a group of patients with pityriasis versicolor and to find out the epidemiological characteristics in this part of India as well as any association, if any, with other diseases. Materials and Methods: For this purpose, 110 consecutive patients of pityriasis versicolor were evaluated clinically and diagnosis was confirmed mycologically at a tertiary care hospital in Kolkata. All data were recorded in a predesigned, pretested semi-structured schedule. The total duration of study period was 12 months. Results: Majority of the patients were young adults. Most of the patients were asymptomatic. There is prominent seasonal variation of the patients with a peak in August and September months. Most of the lesions were hypopigmented scaly macules and were KOH positive. Most commonly involved sites were chest, face and back. Seborrheic dermatitis sometimes coexisted with pityriasis versicolor and a number of patients also had diabetes mellitus and immunosuppressive conditions. Conclusions: Overall, the clinicomycological and epidemiological profile of pityriasis versicolor infection as observed in a tertiary care setting in eastern India does not differ significantly from those observed by previous workers elsewhere.

  9. Corn forage biological pretreatment by Trametes versicolor in a tray bioreactor.

    Science.gov (United States)

    Planinić, Mirela; Zelić, Bruno; Čubel, Ivan; Bucić-Kojić, Ana; Tišma, Marina

    2016-08-01

    Trametes versicolor is a white-rot fungus known to be efficient in lignin removal due to its complex extracellular lignocellulolytic enzymatic system. Therefore, it can be used in the treatment of lignocellulose waste from agro, food, and wood industries. In a first experiment, corn forage treatment with T. versicolor was investigated in laboratory jars. In a second experiment, the process was scaled up to a tray bioreactor. In the tray bioreactor, the process of lignin degradation was improved, resulting in an increase in lignin conversion of up to 71% during seven days' treatment. PMID:27401159

  10. Pitiríase versicolor circinada: isolamento de Malassezia sympodialis - Relato de caso Pityriasis versicolor circinata: isolation of Malassezia sympodialis - Case report

    Directory of Open Access Journals (Sweden)

    Valéria Maria de Souza Framil

    2010-04-01

    Full Text Available Os autores descrevem caso de pitiríase versicolor circinada, cujo agente etiológico isolado foi Malassezia sympodialis em uma mulher de 34 anos. O isolamento e identificação da Malassezia sympodialis foi em ágar Dixon modificado e o método molecular para confirmação da espécie foi PCR-RFLP (polymerase chain reaction and restriction fragment length polymorphism analysis.The authors report a case of pityriasis versicolor circinata whose isolated etiologic agent was Malassezia sympodialis in a 34-year-old woman. The isolation and identification of Malassezia sympodialis were accomplished with modified Dixon's agar, and the molecular method used to confirm the species was polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP.

  11. Laccase activity profiling and gene expression in PCB-degrading cultures of Trametes versicolor

    Czech Academy of Sciences Publication Activity Database

    Plačková, Martina; Svobodová, Kateřina; Cajthaml, Tomáš

    2012-01-01

    Roč. 71, JUL 2012 (2012), s. 22-28. ISSN 0964-8305 R&D Projects: GA ČR GAP503/10/0408; GA ČR GA525/09/1058 Institutional research plan: CEZ:AV0Z50200510 Keywords : Trametes versicolor * PCB * Delor 103 Subject RIV: EE - Microbiology, Virology Impact factor: 2.059, year: 2012

  12. Identification and speciation of Malassezia in patients clinically suspected of having pityriasis versicolor

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    Avani Shah

    2013-01-01

    Full Text Available Background: Malassezia is a lipid-dependent yeast known to cause Pityriasis versicolor, a chronic, recurrent superficial infection of skin and present as hypopigmented or hyperpigmented lesions on areas of skin. If not diagnosed and treated, it may lead to disfigurement of the areas involved and also result in deep invasive infections. Aim: The aim of the present study was to identify and speciate Malassezia in patients clinically suspected of having Pityriasis versicolor. Materials and Methods: Total 139 patients suspected of having Pityriasis versicolor were evaluated clinically and diagnosis was done by Wood′s lamp examination, confirmed mycologically by using KOH, cultivation on Sabouraud′s dextrose agar and modified Dixon agar at a tertiary care hospital in Mumbai. The total duration of study was 12 months. Results: Majority of the patients were males (59.71% in the age group of 21-30 years (33.81% who were students (30.21% by profession. The incidence of Malassezia in Pityriasis versicolor was 50.35%. The most common isolate was M. globosa (48.57%, followed by M. furfur (34.28%. Majority of the patients had hypopigmented lesions, with M. globosa as the predominant isolate. Neck was the most common site affected; 88.48% were Wood′s lamp positive of which 56.91% of Malassezia isolates grew on culture. KOH mount was positive in 82.01% of which 61.40% Malassezia isolates grew on culture. Conclusions: The procedure of culture and antifungal testing is required to be performed as different species of Malassezia are involved in Pityriasis versicolor and susceptibility is different among different species. Thus, it would help to prevent recurrences and any systemic complications.

  13. DESTRUCTION OF ASPERGILLUS VERSICOLOR, PENICILLIUM CRYSOGENUM, STACHYBOTRYS CHARTARUM, AND CLADOSPORIUM CLADOSPORIDES SPORES USING CHEMICAL OXIDATION TREATMENT PROCESS

    Science.gov (United States)

    The survival of aqueous suspensions of Penicillium chrysogenum, Stachybotrys chartarum, Aspergillus versicolor, and Cladosporium cladosporioides spores was evaluated using various combinations of hydrogen peroxide and iron (II) as catalyst. Spores were suspended in water and trea...

  14. Treatment of pityriasis versicolor with topical application of essential oil of Cymbopogon citratus (DC) Stapf - therapeutic pilot study*

    OpenAIRE

    Carmo, Egberto Santos; Pereira, Fillipe de Oliveira; Cavalcante, Neuza Maria; Gayoso, Carla Wanderley; Lima, Edeltrudes de Oliveira

    2013-01-01

    BACKGROUND Pityriasis versicolor is a fungal infection caused by Malassezia spp. that has frequent relapses. OBJECTIVES The main objective of this research was to perform phase I and II clinical studies, using formulations containing essential oil of Cymbopogon citratus in patients with pityriasis versicolor. METHODS Phase I study included twenty volunteers to ascertain the safety of the formulations. In phase II, 47 volunteers randomly received essential oil formulations at 1.25 μL/mL concen...

  15. Biodegradation and utilization of 4-n-nonylphenol by Aspergillus versicolor as a sole carbon and energy source

    Energy Technology Data Exchange (ETDEWEB)

    Krupiński, Mariusz; Janicki, Tomasz [Department of Industrial Microbiology and Biotechnology, University of Lodz, Banacha 12/16, 90-237 Łódź (Poland); Pałecz, Bartłomiej [Department of Physical Chemistry, University of Lodz, Pomorska 165, 90-236 Łódź (Poland); Długoński, Jerzy, E-mail: jdlugo@biol.uni.lodz.pl [Department of Industrial Microbiology and Biotechnology, University of Lodz, Banacha 12/16, 90-237 Łódź (Poland)

    2014-09-15

    Highlights: • A. versicolor is able to degrade 4-n-NP as the sole source of carbon and energy. • 4-n-NP removal by A. versicolor was accompanied by the formation of metabolites. • Radioactive experiments show complete 4-n-NP mineralization by A. versicolor. • 4-n-NP initiates heat production in the A. versicolor spores. - Abstract: 4-n-Nonylphenol (4-n-NP) is an environmental pollutant with endocrine-disrupting activities that is formed during the degradation of nonylphenol polyethoxylates, which are widely used as surfactants. Utilization of 4-n-NP by the filamentous fungus Aspergillus versicolor as the sole carbon and energy source was investigated. By means of gas chromatography–mass spectrometry, we showed that in the absence of any carbon source other than 4-n-NP in the medium, A. versicolor completely removed the xenobiotic (100 mg L{sup −1}) after 3 d of cultivation. Moreover, mass spectrometric analysis of intracellular extracts led to the identification of eight intermediates. The mineralization of the xenobiotic in cultures supplemented with 4-n-NP [ring-{sup 14}C(U)] as a growth substrate was also assessed. After 3 d of incubation, approximately 50% of the initially applied radioactivity was recovered in the form of {sup 14}CO{sub 2}, proving that this xenobiotic was completely metabolized and utilized by A. versicolor as a carbon source. Based on microscopic analysis, A. versicolor is capable of germinating spores under such conditions. To confirm these observations, a microcalorimetric method was used. The results show that even the highest amount of 4-n-NP initiates heat production in the fungal samples, proving that metabolic processes were affected by the use of 4-n-NP as an energetic substrate.

  16. Starch degradation and nutrition value improvement in corn grits by solid state fermentation technique with Coriolus versicolor

    OpenAIRE

    Huang, Mian; Zhang, Song

    2011-01-01

    The study was conducted to evaluate effect of Coriolus versicolor mycelia on degrading starch and improving nutrition value in corn grits through solid state fermentation technique. The results showed that using soybean meal as a nitrogen source, α-amylase secreted from C. versicolor expressed 407.25U/g of activity, leading to 45.15% of starch degraded. The activity grew with fermentation time until the 15th day, after that the amylase was deactivated rapidly. An orthogonal experiment designe...

  17. Starch degradation and nutrition value improvement in corn grits by solid state fermentation technique with Coriolus versicolor

    OpenAIRE

    Mian Huang; Song Zhang

    2011-01-01

    The study was conducted to evaluate effect of Coriolus versicolor mycelia on degrading starch and improving nutrition value in corn grits through solid state fermentation technique. The results showed that using soybean meal as a nitrogen source, α-amylase secreted from C. versicolor expressed 407.25U/g of activity, leading to 45.15% of starch degraded. The activity grew with fermentation time until the 15th day, after that the amylase was deactivated rapidly. An orthogonal experiment de...

  18. Biodegradation and utilization of 4-n-nonylphenol by Aspergillus versicolor as a sole carbon and energy source

    International Nuclear Information System (INIS)

    Highlights: • A. versicolor is able to degrade 4-n-NP as the sole source of carbon and energy. • 4-n-NP removal by A. versicolor was accompanied by the formation of metabolites. • Radioactive experiments show complete 4-n-NP mineralization by A. versicolor. • 4-n-NP initiates heat production in the A. versicolor spores. - Abstract: 4-n-Nonylphenol (4-n-NP) is an environmental pollutant with endocrine-disrupting activities that is formed during the degradation of nonylphenol polyethoxylates, which are widely used as surfactants. Utilization of 4-n-NP by the filamentous fungus Aspergillus versicolor as the sole carbon and energy source was investigated. By means of gas chromatography–mass spectrometry, we showed that in the absence of any carbon source other than 4-n-NP in the medium, A. versicolor completely removed the xenobiotic (100 mg L−1) after 3 d of cultivation. Moreover, mass spectrometric analysis of intracellular extracts led to the identification of eight intermediates. The mineralization of the xenobiotic in cultures supplemented with 4-n-NP [ring-14C(U)] as a growth substrate was also assessed. After 3 d of incubation, approximately 50% of the initially applied radioactivity was recovered in the form of 14CO2, proving that this xenobiotic was completely metabolized and utilized by A. versicolor as a carbon source. Based on microscopic analysis, A. versicolor is capable of germinating spores under such conditions. To confirm these observations, a microcalorimetric method was used. The results show that even the highest amount of 4-n-NP initiates heat production in the fungal samples, proving that metabolic processes were affected by the use of 4-n-NP as an energetic substrate

  19. Role of laccase and low molecular weight metabolites from Trametes versicolor in dye decolorization.

    Science.gov (United States)

    Moldes, Diego; Fernández-Fernández, María; Sanromán, M Ángeles

    2012-01-01

    The studies regarding decolorization of dyes by laccase may not only inform about the possible application of this enzyme for environmental purposes, but also may provide important information about its reaction mechanism and the influence of several factors that could be involved. In this paper, decolorization of crystal violet and phenol red was carried out with different fractions of extracellular liquids from Trametes versicolor cultures, in order to describe the role of laccase in this reaction. Moreover, the possible role of the low molecular weight metabolites (LMWMs) also produced by the fungus was evaluated. The results confirm the existence of a nonenzymatic decolorization factor, since the nonprotein fraction of the extracellular liquids from cultures of T. versicolor has shown decolorization capability. Several experiments were performed in order to identify the main compounds related to this ability, which are probably low molecular weight peroxide compounds. PMID:22566767

  20. Over production of lignocellulosic enzymes of Coriolus versicolor by genetic engineering methodology. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Williams, A.L.

    1998-07-01

    The project seeks to understand the biological and chemical processes involved in the secretion of the enzyme polyphenol oxidase (PPO) by the hyphae, the basic unit of the filamentous fungus Coriolus versicolor. These studies are made to determine rational strategies for enhanced secretion of PPO, both with the use of recombinant DNA techniques and without. This effort focuses on recombinant DNA techniques to enhance enzyme production. The major thrust of this project was two-fold: to mass produce C. versicolor tyrosinase (polyphenol oxidase) by genetic engineering as well as cultural manipulations; and to utilize PPO as a biocatalyst in the processing of lignocellulose as a renewable energy resource. In this study, the assessment of genomic and cDNA recombinant clones with regards to the overproduction of PPO continued. Further, immunocytochemical techniques were employed to assess the mechanism(s) involved in the secretion of PPO by the hyphae. Also, factors influencing PPO secretion were examined.

  1. Novel Chromone Derivatives from Marine Fungus Aspergillus versicolor Isolated from the Sponge Xestospongia exigua

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    From the marine sponge Xestospongia exigua, fungal isolates of Aspergillus versicolor (Vuill)Triab were obtained. Isolation and purification of ethyl acetate extracts from culture filtrates of the fungus led to yield six new chromone derivatives namely aspergione A, aspergione B, aspergione C, aspergione D, aspergione E, aspergione F. The structures of all the new compounds were established on the basis of extensive spectroscopy (UV, MS, 1H and 13C NMR, COSY, HMQC and HMBC) analysis.

  2. Role of Laccase and Low Molecular Weight Metabolites from Trametes versicolor in Dye Decolorization

    OpenAIRE

    Diego Moldes; María Fernández-Fernández; M. Ángeles Sanromán

    2012-01-01

    The studies regarding decolorization of dyes by laccase may not only inform about the possible application of this enzyme for environmental purposes, but also may provide important information about its reaction mechanism and the influence of several factors that could be involved. In this paper, decolorization of crystal violet and phenol red was carried out with different fractions of extracellular liquids from Trametes versicolor cultures, in order to describe the role of laccase in this r...

  3. Phase 1 Clinical Trial of Trametes versicolor in Women with Breast Cancer

    OpenAIRE

    Amy Putiri; Standish, Leanna J.; Juliette Gay; WENNER, CYNTHIA A.; Masa Sasagawa; Erin Sweet; Martzen, Mark R; Carolyn J. Torkelson

    2012-01-01

    Introduction. Orally administered preparations from the Trametes versicolor (Tv) mushroom have been hypothesized to improve immune response in women with breast cancer after standard chemotherapy and radiotherapy. Methods. A phase I, two-center, dose escalation study was done to determine the maximum tolerated dose of a Tv preparation when taken daily in divided doses for 6 weeks after recent completion of radiotherapy. Eleven participants were recruited and nine women completed the study. Ea...

  4. Treatment of a Textile Effluent from Dyeing with Cochineal Extracts Using Trametes versicolor Fungus

    OpenAIRE

    Gabriela Arroyo-Figueroa; Graciela M. L. Ruiz-Aguilar; Leticia López-Martínez; Guillermo González-Sánchez; Germán Cuevas-Rodríguez; Refugio Rodríguez-Vázquez

    2011-01-01

    Trametes versicolor (Tv) fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. However, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. Experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of Tv fungus were done. Treatments to determine decolorization in t...

  5. Symbiosis initiation in the bacterially luminous sea urchin cardinalfish Siphamia versicolor.

    Science.gov (United States)

    Dunlap, P V; Gould, A L; Wittenrich, M L; Nakamura, M

    2012-09-01

    To determine how each new generation of the sea urchin cardinalfish Siphamia versicolor acquires the symbiotic luminous bacterium Photobacterium mandapamensis, and when in its development the S. versicolor initiates the symbiosis, procedures were established for rearing S. versicolor larvae in an aposymbiotic state. Under the conditions provided, larvae survived and developed for 28 days after their release from the mouths of males. Notochord flexion began at 8 days post release (dpr). By 28 dpr, squamation was evident and the caudal complex was complete. The light organ remained free of bacteria but increased in size and complexity during development of the larvae. Thus, aposymbiotic larvae of the fish can survive and develop for extended periods, major components of the luminescence system develop in the absence of the bacteria and the bacteria are not acquired directly from a parent, via the egg or during mouth brooding. Presentation of the symbiotic bacteria to aposymbiotic larvae at 8-10 dpr, but not earlier, led to initiation of the symbiosis. Upon colonization of the light organ, the bacterial population increased rapidly and cells forming the light-organ chambers exhibited a differentiated appearance. Therefore, the light organ apparently first becomes receptive to colonization after 1 week post-release development, the symbiosis is initiated by bacteria acquired from the environment and bacterial colonization induces morphological changes in the nascent light organ. The abilities to culture larvae of S. versicolor for extended periods and to initiate the symbiosis in aposymbiotic larvae are key steps in establishing the experimental tractability of this highly specific vertebrate and microbe mutualism. PMID:22957874

  6. Comparative study of ketoconazole versus selenium sulphide shampoo in pityriasis versicolor

    OpenAIRE

    Aggarwal K; Jain V; Sangwan S

    2003-01-01

    Forty patients suffering from pityriasis versicolor were treated with either 2% ketoconazole shampoo (20 patients) or 2.5% selenium sulphide shampoo (20 patients), once a week for three weeks. On global assessment after one month of start of therapy, 19 (95%) out of 20 patients treated with ketoconazole shampoo were cured while one case had mild residual disease. In selenium sulphide shampoo group, 17 (85%) out of 20 patients were cured, one had mild residual dise...

  7. Efficacy of Edible Film Incorporated with Essential Oils against White-rot Decay Fungus (Trametes versicolor)

    OpenAIRE

    Saifon Phothisuwan; Narumol Matan

    2013-01-01

    Antifungal activities of edible film incorporated with essential oils (cinnamon oil, clove oil, anise oil, citronella oil, orange oil, tangerine oil, turmeric oil, guava leave oil, nutmeg oil and lime oil) against a white-rot decay fungus (Trametes versicolor) identified from rubberwood were investigated. The disc dilution method was employed to determine the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) by mixing edible film with essential oil at ratios 1:...

  8. Invasive pulmonary aspergillosis caused by Aspergillus versicolor in a patient on mechanical ventilation

    OpenAIRE

    MV Pravin Charles; M Ravishankar; Easow, Joshy M; Noyal Mariya Joseph

    2011-01-01

    Aspergillus spp. often colonise the respiratory tract of critically ill patients in intensive care units and subsequently cause invasive disease. The risk of developing invasive disease is more in immunocompromised patients. Here we report a case of fatal invasive pulmonary aspergillosis caused by Aspergillus versicolor in a post-operative patient on mechanical ventilation, who did not respond to intravenous itraconazole. We then discuss the challenges involved in the accurate diagnosis of th...

  9. Antimicrobial Activity of the Macrofungus Coriolus versicolor (L. ex Fr.) Quel

    OpenAIRE

    Başaran DÜLGER

    1999-01-01

    In this study, ethyl acetate, acetone, chloroform and ethanol extracts of Coriolus versicolor (L. ex Fr.) Quel. were tested for antimicrobial activity by Disc Diffusion method on the following test microorganisms: Aeromonas hydrophila ATCC 7966, Listeria monocytogenes ATCC 19117, Escherichia coli ATCC 11230, Enterobacter aerogenes ATCC 13048, Corynebacterium xerosis CCM 2824, Corynebacterium glutamicum ATCC 13022, Proteus vugaris ATCC 8427, Serratia marcescens NRRL 3284, Bacillus cereus ...

  10. Partial purification of coriolus versicolor's extracellular polyphenol oxidase (PPO)

    Energy Technology Data Exchange (ETDEWEB)

    Moore, N.L.; Dashek, W.V. (Clark Atlanta Univ., GA (United States))

    1993-05-01

    Coriolus versicolor, a white-rot basidiomycete, secretes ligno-celluloytic enzymes. Because these are valuable to paper-pulp agricultural industries, trials are in progress to substrate induce these enzymes enhance their secretions. Reported are attempts to develop an extracellular PPO (o-diphenols to 0-diquinones) purification protocol applicable to [open quote]batch-cultured[close quote] C. versicolor. Whereas dialysis (MW [open quote]cut-off[close quote], 14,000) of 13 day growth medium (GM) resulted in 2.17 fold PPO spc. act. increase, dialysis plus a 0-30% (NH[sub 4])[sub 2]SO[sub 4] [open quote]cut[close quote] yielded a 3.27 fold enhancement. Subsequent GM chromatography on DEAE CM-Sephadexes revealed that PPO exchanged with DEAE's counterion without enhancing spc. act. Gel filtration of GM commercial PPOs on G-150 resulted in similar elutions indicating a substitute for ion exchange chromatography. Time-dependent fungal growth in liquid medium followed by viscometry utilizing CMC revealed a GM endocellulase 2 days after inoculation an activity rise to day 12. Filteration of Onozuka cellulase on G-150 yielded an elution profile similar to those of GM authentic PPO's compounding C. versicolor's PPO purification. SDS-PAGE of dialyzed GM revealed 4 proteins, one of which was removed by the (NH[sub 4])[sub 2]SO[sub 4]. The m[sub TS] of commercial Sigma's PPO Onozuka cellulase were 0.76 0.59, respectively, for comparison to C. versicolor's PPO. Affinity, hydroxylapatite hydrophobic interaction chromatographies may yield a single SDS-PAGE PPO band.

  11. Factors influencing offspring traits in the oviparous multi-clutched lizard, Calotes versicolor (Agamidae)

    Indian Academy of Sciences (India)

    Rajkumar S Radder; Bhagyashri A Shanbhag

    2004-03-01

    The determinants of offspring size and number in the tropical oviparous multi-clutched lizard, Calotes versicolor, were examined using both univariate and multivariate (path) analyses. In C. versicolor maternal snout-vent length (SVL) and body condition influence clutch mass and clutch size but have no significant influence on offspring size. The positive effect of maternal SVL and body condition on offspring number is counterbalanced by a negative effect of breeding time on egg mass. In fact, breeding time directly influences the offspring body mass and condition through variation in the egg mass. There is a trade-off between offspring mass and condition with offspring number, and breeding time influences both. Offspring hatched from the eggs of early (May–June) or mid (July–August) breeding periods invariably show lower mass and condition than those hatched from the eggs of late breeding season (September–October). Yet, there is no variation in offspring SVL among early, mid and late clutches. Thus, in C. versicolor offspring SVL is optimized while body mass and condition are not optimized.

  12. 云芝多糖研究进展%Review on Polysaccharide from Coriolus versicolor

    Institute of Scientific and Technical Information of China (English)

    王菲菲; 郝利民; 贾士儒; 陈强; 牛爽

    2012-01-01

    In recent years,edible and medicinal fungi are paid more and more attention for their various biological functions and thus become a research focus.Polysaccharide of Coriolus versicolor is isolated from Coriolus versicolor or its broth,and took more interesting from the industries of medicine,food,cosmetics and even chemistry and so on because of its superior biological activity and functions.This is a review on the polysaccharide structure,properties,production,usage,market of polysaccharide from Coriolus versicolor.It's also a comprehensive survey and reference of polysaccharide for further research and development.%近年来,药食用真菌因具有多种生物学功能而受到重视,成为研究热点。云芝多糖由云芝或从其发酵液中分离提取,具有突出的药食用功效和生物活性。文中从云芝多糖的结构、性质、提取纯化、发酵生产、用途与展望等方面进行了综述,为云芝多糖的进一步研究与开发提供参考。

  13. Comparative Efficacy of 10% Sodium Sulfacetamide Lotion and 1% Clotrimazole Lotion in Treatment of Tinea versicolor

    Directory of Open Access Journals (Sweden)

    F Rad

    2008-01-01

    Full Text Available ABSTRACT: Introduction & Objective: Tinea versicolor is a common superficial fungal infection caused by normal skin flora and many topical and oral anti-fungal medications have been used for its treatment. The aim of this study was to compare the therapeutic effects of 10% sodium sulfacetamide lotion with 1% clotrimazole lotion in treatment of tinea versicolor. Materials & Methods: In this single-blind clinical trial 67 patients were divided into two groups using block randomization method. The first group consisted of 33 patients who were treated with 1% clotrimazole lotion. The second group included 34 patients who received 10% sodium sulfacetamide lotion. After 2 weeks of treatment, both groups were evaluated for clinical and paraclinical responses. The collected data were recorded in a data sheet and analyzed by X2 and independent T tests, using SPSS software. Results: After 2 weeks of treatment, clinical and paraclinical responses were seen in 22 patients (66.7% and 28 patients (84.8% in the first group (clotrimazole lotion and in 7 (20.6% and 8 (23.5% patients of the second group (sodium sulfacetamide lotion, respectively. Statistical analysis showed that the differences are significant. Conclusion: The therapeutic effect of 10% sodium sulfacetamide lotion is much less than that of 1% clotrimazole lotion in treatment of tinea versicolor.

  14. Prevalence of different Malassezia species in pityriasis versicolor in central India

    Directory of Open Access Journals (Sweden)

    Chaudhary Rahul

    2010-01-01

    Full Text Available Background: In the last 10 years, different studies have shown interesting geographical variations in the prevalence of different Malassezia species in pityriasis versicolor. Aim: Identification of Malassezia species isolated from patients with pityriasis versicolor. Methods: In 100 patients with pityriasis versicolor, Malassezia species were identified by culture in Sabouraud′s dextrose agar containing cycloheximide with olive oil overlay and modified Dixon agar and by doing biochemical tests (catalase reaction, assimilation of glycine, and Tween utilisation tests. Results: In 10 patients, 10% KOH smear was negative, while in 90 patients the smear showed characteristic "spaghetti and meatball" appearance. Of these 90 cases, growth was obtained on modified Dixon′s agar in 87 cases. Fifty of the isolates (57.5% were M. globosa, 15 (17.2% were M. sympodialis, seven (8.0% were suspected M. sympodialis, 6 (6.9% each of the isolates were M. furfur and M. obtusa, and three (3.4% isolates were M. restricta. Conclusion: M. globosa was the most common species, followed by M. sympodialis, M. furfur, M. obtusa, and M. restricta.

  15. Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tehran, Iran

    Directory of Open Access Journals (Sweden)

    Zeraati Hojjat

    2004-05-01

    Full Text Available Abstract Background Pityriasis versicolor is a superficial infection of the stratum corneum which caused by a group of yeasts formerly named pityrosporium. The taxonomy of these lipophilic yeasts has recently been modified and includes seven species referred as Malassezia. The aim of this study is to compare the distribution of Malassezia species isolated from pityriasis versicolor lesions and those isolated from healthy skins. Methods Differentiation of all malassezia species performed using morphological features and physiological test including catalase reaction, Tween assimilation test and splitting of esculin. Results In pityriasis versicolor lesions, the most frequently isolated species was M. globosa (53.3%, followed by M. furfur (25.3%, M. sympodialis(9.3%, M. obtusa (8.1% and M. slooffiae (4.0%. The most frequently isolated species in the skin of healthy individuals were M. globosa, M. sympodialis, M. furfur, M. sloofiae and M. restricta which respectively made up 41.7%, 25.0%, 23.3%, 6.7% and 3.3% of the isolated species. Conclusions According to our data, M. globosa was the most prevalent species in the skin of healthy individuals which recovered only in the yeast form. However, the Mycelial form of M. globosa was isolated as the dominant species from pityriasis versicolor lesions. Therefore, the role of predisposing factors in the conversion of this yeast to mycelium and its subsequent involvement in pityriasis versicolor pathogenicity should be considered.

  16. Bioaugmentation of sewage sludge with Trametes versicolor in solid-phase biopiles produces degradation of pharmaceuticals and affects microbial communities.

    Science.gov (United States)

    Rodríguez-Rodríguez, Carlos E; Jelić, Aleksandra; Pereira, M Alcina; Sousa, Diana Z; Petrović, Mira; Alves, M Madalena; Barceló, Damià; Caminal, Glòria; Vicent, Teresa

    2012-11-01

    The use of sludge (biosolids) in land application may contribute to the spread of organic micropollutants as wastewater treatments do not completely remove these compounds. Therefore, the development of alternative strategies for sludge treatment is a matter of recent concern. The elimination of pharmaceuticals at pre-existent concentrations from sewage sludge was assessed, for the first time, in nonsterile biopiles by means of fungal bioaugmentation with Trametes versicolor (BTV-systems) and compared with the effect of autochthonous microbiota (NB-systems). The competition between the autochthonous fungal/bacterial communities and T. versicolor was studied using denaturing gradient gel electrophoresis (DGGE) and the cloning/sequencing approach. An inhibitory effect exerted by T. versicolor over bacterial populations was suggested. However, after 21 days, T. versicolor was no longer the main taxon in the fungal communities. The elimination profiles revealed an enhanced removal of atorvastatin-diclofenac-hydrochlorothiazide (during the whole treatment) and ranitidine-fenofibrate (at short periods) in the BTV biopiles in respect to NB biopiles, coincident with the presence of the fungus. For ibuprofen-clarithromycin-furosemide, the elimination profiles were similar irrespective of the system, and with carbamazepine no significant degradation was obtained. The results suggest that a fungal treatment with T. versicolor could be a promising process for the remediation of some pharmaceuticals in complex matrices such as biosolids. PMID:23030544

  17. Carbon and nitrogen balance of leaf-eating sesarmid crabs ( Neoepisesarma versicolor) offered different food sources

    Science.gov (United States)

    Thongtham, Nalinee; Kristensen, Erik

    2005-10-01

    Carbon and nitrogen budgets for the leaf-eating crab, Neoepisesarma versicolor, were established for individuals living on pure leaf diets. Crabs were fed fresh (green), senescent (yellow) and partly degraded (brown) leaves of the mangrove tree Rhizophora apiculata. Ingestion, egestion and metabolic loss of carbon and nitrogen were determined from laboratory experiments. In addition, bacterial abundance in various compartments of the crabs' digestive tract was enumerated after dissection of live individuals. Ingestion and egestion rates (in terms of dry weight) were highest, while the assimilation efficiency was poorest for crabs fed on brown leaves. The low assimilation efficiency was more than counteracted by the high ingestion rate providing more carbon for growth than for crabs fed green and yellow leaves. In any case, the results show that all types of leaves can provide adequate carbon while nitrogen was insufficient to support both maintenance (yellow leaves) and growth (green, yellow and brown leaves). Leaf-eating crabs must therefore obtain supplementary nitrogen by other means in order to meet their nitrogen requirement. Three hypotheses were evaluated: (1) crabs supplement their diet with bacteria and benthic microalgae by ingesting own faeces and/or selective grazing at the sediment surface; (2) assimilation of symbiotic nitrogen-fixing bacteria in the crabs' own intestinal system; and (3) nitrogen storage following occasional feeding on animal tissues (e.g. meiofauna and carcasses). It appears that hypothesis 1 is of limited importance for N. versicolor since faeces and sediment can only supply a minor fraction of the missing nitrogen due to physical constraints on the amount of material the crabs can consume. Hypothesis 2 can be ruled out because tests showed no nitrogen fixation activity in the intestinal system of N. versicolor. It is therefore likely that leaf-eating crabs provide most of their nitrogen requirement from intracellular deposits

  18. Coleoptera Associated with Decaying Wood in a Tropical Deciduous Forest.

    Science.gov (United States)

    Muñoz-López, N Z; Andrés-Hernández, A R; Carrillo-Ruiz, H; Rivas-Arancibia, S P

    2016-08-01

    Coleoptera is the largest and diverse group of organisms, but few studies are dedicated to determine the diversity and feeding guilds of saproxylic Coleoptera. We demonstrate the diversity, abundance, feeding guilds, and succession process of Coleoptera associated with decaying wood in a tropical deciduous forest in the Mixteca Poblana, Mexico. Decaying wood was sampled and classified into four stages of decay, and the associated Coleoptera. The wood was identified according to their anatomy. Diversity was estimated using the Simpson index, while abundance was estimated using a Kruskal-Wallis test; the association of Coleoptera with wood species and decay was assessed using canonical correspondence analysis. Decay wood stage I is the most abundant (51%), followed by stage III (21%). We collected 93 Coleoptera belonging to 14 families, 41 genera, and 44 species. The family Cerambycidae was the most abundant, with 29% of individuals, followed by Tenebrionidae with 27% and Carabidae with 13%. We recognized six feeding guilds. The greatest diversity of Coleoptera was recorded in decaying Acacia farnesiana and Bursera linanoe. Kruskal-Wallis analysis indicated that the abundance of Coleoptera varied according to the species and stage of decay of the wood. The canonical analysis showed that the species and stage of decay of wood determined the composition and community structure of Coleoptera. PMID:26911160

  19. Function of the iron-binding chelator produced by Coriolus versicolor in lignin biodegradation

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    An ultrafiltered low-molecular-weight preparation of chelating compounds was isolated from a wood-containing culture of the white-rot basidiomycete Coriolus versicolor. This preparation could chelate Fe3+ and reduce Fe3+ to Fe2+, demonstrating that the substance may serve as a ferric chelator, oxygen-reducing agent, and redox-cycling molecule, which would include functioning as the electron transport carrier in Fenton reaction. Lignin was treated with the iron-binding chelator and the changes in structure were investigated by 1H-NMR, 13C-NMR, difference spectrum caused by ionization under alkaline conditions and nitrobenzene oxidation. The results indicated that the iron-binding chelator could destroy the β-O-4 bonds in etherified lignin units and insert phenolic hydroxyl groups. The low-molecular-weight chelator secreted by C. versicolor resulted in new phenolic substructures in the lignin polymer, making it susceptible to attack by laccase or manganese peroxidase. Thus, the synergic action of the iron-binding chelator and the lignocellulolytic enzymes made the substrate more acces- sible to degradation.

  20. Antifungal activity of oregano extract against A. Versicolor, E. Nidulans and Eurotium spp.: Producers of sterigmatocystin

    Directory of Open Access Journals (Sweden)

    Kocić-Tanackov Sunčica D.

    2011-01-01

    Full Text Available The paper presents the influence of oregano extract (Origanum vulgare L. on growth of Aspergillus versicolor, Emericella nidulans, Eurotium herbariorum, E. amstelodami, E. chevalieri and E. rubrum - producers of sterigmatocystin (STC isolated from salads. Antifungal tests were performed by agar plates method. The composition of the active component of extract was determined by GC-MS method and the major components were: carvacrol (34.20%, triacetin (22.91%, carvone (18.05%, p-cymene (8.05% and thymol (3.74%. The examined extract showed the ability to reduce mold growth at all applied concentrations. Minimum inhibitory concentrations (MIC for E. nidulans, E. chevalieri and E. amstelodami were 2.5% (v/v and over 2.5% (v/v for A. versicolor. At 1.5% (v/v concentration the extract completely inhibited the growth of E. rubrum, whereas higher dose of 2.5% (v/v was fungicidal against E. herbariorum. Besides its sensory role in food products, the examined oregano extract also exhibits antifungal activities against producers of STC.

  1. Hormonal modulation of phonotaxis and advertisement-call preferences in the gray treefrog (Hyla versicolor)

    Science.gov (United States)

    Gordon, Noah M.; Gerhardt, H. Carl

    2009-01-01

    Hormonal levels fluctuate during the breeding season in many anurans, but the identity of the hormones that modulate breeding behavior and their effects remain unclear. We tested the influence of a combined treatment of progesterone and prostaglandin on phonotaxis, the key proceptive reproductive behavior of female anurans. First, we found that female gray treefrogs (Hyla versicolor) treated with progesterone and prostaglandin exhibited phonotaxis to synthetic male advertisement signals significantly more often than animals treated with ringers vehicle or uninjected controls. Responsive females had greater levels of plasma progesterone and estradiol compared to both control groups, suggesting that these steroids may be promoting phonotaxis. Second, we found that the selectivity of hormonally-induced phonotaxis in H. versicolor was similar to that observed in freshly captured breeding animals. Females made the same choices between acoustic signals after hormone treatments in tests of frequency, call rate and pulse rate, compared to their responses without treatment immediately after collection from the breeding chorus. The preference for a longer call was, however, significantly weaker after hormone induction of phonotaxis. Hormonally primed females were also less likely to respond in any test and took longer to respond than did freshly collected females. Consequently, our study shows how progesterone-prostaglandin induced phonotaxis in female treefrogs influences both the quality and quantity of phonotaxis, relative to that exhibited by naturally breeding females. PMID:18926827

  2. Effect Of Metal Ions On Triphenylmethane Dye Decolorization By Laccase From Trametes Versicolor

    Directory of Open Access Journals (Sweden)

    Chmelová Daniela

    2015-12-01

    Full Text Available The aim of this study was investigate the influence of different metal ions on laccase activity and triphenylmethane dye decolorization by laccase from white-rot fungus Trametes versicolor. Laccase activity was inhibited by monovalent ions (Li+, Na+, K+ and Ag+ but the presence of divalent ions increased laccase activity at the concentration of 10 mmol/l. The effect of metal ions on decolorization of triphenylmethane dyes with different structures namely Bromochlorophenol Blue, Bromophenol Blue, Bromocresol Blue and Phenol Red was tested. The presence of metal ions (Na+, K+, Mg2+, Ca2+, Ba2+, Mn2+, Zn2+ slightly decreased triphenylmethane dye decolorization by laccase from T. versicolor except Na+ and Mg2+, which caused the increase of decolorization for all tested dyes. Decolorization of selected dyes showed that the presence of low-molecular-weight compounds is necessary for effective decolorization. Hydroxybenzotriazole (HBT is the most frequently used. Although HBT belongs to most frequently used redox mediator and generally increase decolorization efficiency, so its presence decreased decolorization percentage of Bromophenol Blue and Bromochlorophenol Blue, the influence of metal ions to dye decolorization by laccase has the similar course with or without presence of redox mediator HBT.

  3. Walking to survive. Searching, feeding and egg production of the carabid beetle Pterostichus coerulescens L. (= Poecilus versicolor Sturm).

    NARCIS (Netherlands)

    Mols, P.J.M.

    1993-01-01

    This study concerns the prey-searching and feeding behaviour of the polyphagous groundbeetle Pterostichus coerulescens L. ( = Poecilus versicolor Sturm), a common species on sandy soils. This ground beetle rarely flies, thus preysearching behaviour involves walking. The beetle is diurnal. As object

  4. The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis

    Science.gov (United States)

    Matijašević, Danka; Pantić, Milena; Rašković, Božidar; Pavlović, Vladimir; Duvnjak, Dunja; Sknepnek, Aleksandra; Nikšić, Miomir

    2016-01-01

    The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL-1. C. versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms. PMID:27540376

  5. Tanyproctini (Coleoptera: Scarabaeidae: Melolonthinae) of Socotra Island

    Czech Academy of Sciences Publication Activity Database

    Král, D.; Sehnal, R.; Bezděk, Aleš

    2012-01-01

    Roč. 52, suppl. 2 (2012), s. 153-182. ISSN 0374-1036 Grant ostatní: Ministry of Education, Youth and Sports (CZ) LA10036/MSMT Institutional support: RVO:60077344 Keywords : Coleoptera * Scarabaeidae * Melolonthinae Subject RIV: EG - Zoology Impact factor: 0.963, year: 2012 http://www.aemnp.eu/PDF/52_s2/52_S2_153.pdf

  6. IMPROVING ENZYMATIC SACCHARIFICATION OF SUGARCANE BAGASSE BY BIOLOGICAL/PHYSICO-CHEMICAL PRETREATMENT USING TRAMETES VERSICOLOR AND BACILLUS SP.

    Directory of Open Access Journals (Sweden)

    Chularat Krongtaew Sakdaronnarong,

    2012-07-01

    Full Text Available In this work, laccase biosynthesis of two microorganisms, Trametes versicolor TISTR 3224 and Bacillus sp. TISTR 908 isolated in Thailand, was investigated using sugarcane bagasse (SCB as substrate. Two-stage biological/physico-chemical pretreatment of SCB on delignification and saccharification yield was studied. A two-level full factorial design was applied and 3 factors influencing delignification and saccharification processes of SCB were studied including C:N ratio (10:1 to 20:1, temperature (100 to 140°C, and alkali concentration (0 to 5% w/w NaOH. It was found that during biological pretreatment of SCB, a greater amount of laccase was produced from T. versicolor in the early stage of growth compared with Bacillus sp. Nitrogen supplement enhanced laccase biosynthesis of T. versicolor. By contrast, Bacillus sp. required a smaller amount of nitrogen source to produce laccase. Biological treated bagasse was subsequently subjected to a physico-chemical treatment. The results showed that the highest xylose and glucose yield of 51.97% w/w based on carbohydrate content was obtained from T. versicolor cultivation at a C:N ratio of 20:1, and consecutively treated in 5% w/w NaOH solution at 140°C for 1 h. Bacterial/alkali and alkali pretreatment yielded xylose and glucose in smaller degrees compared with fungal/alkali pretreatment. T. versicolor preferentially degraded lignin in sugarcane bagasse relative to cellulose and hemicelluloses constituents, while Bacillus sp. simultaneously attacked both lignin and carbohydrate moieties, as indicated by analysis of relative FT-IR intensities ratios of pretreated and untreated sugarcane bagasse.

  7. Leaf removal by sesarmid crabs in Bangrong mangrove forest, Phuket, Thailand; with emphasis on the feeding ecology of Neoepisesarma versicolor

    Science.gov (United States)

    Thongtham, Nalinee; Kristensen, Erik; Puangprasan, Som-Ying

    2008-12-01

    Field measurements on leaf removal by populations of sesarmid crabs at different locations in the Bangrong mangrove forest, Phuket, Thailand, indicated that crabs on average can remove 87% of the daily leaf litter fall by ingestion or burial. The removal rate is correlated positively with the number of crab burrows and negatively with tidal inundation time. The results from the field were supplemented with observations on the behavior of Neoepisesarma versicolor in laboratory microcosms and a mangrove mesocosm. N. versicolor feeds primarily at night and total time spent feeding was up to an order of magnitude higher in the artificial microcosms than under simulated in situ conditions in the mesocosm. Most of the time during both day and night was spent resting near the entrance or inside burrows. N. versicolor mainly feeds on mangrove leaves and scraps of food material from the sediment surface. This is supported by examinations of stomach content, which showed that 62% is composed of higher plant material and 38% of detritus and mineral particles from the sediment. The nutritive value of leaves and detritus is insufficient to maintain crab growth. Sesarmid crabs may instead obtain the needed nutrients by occasional consumption of nitrogen-rich animal tissues, such as carcasses of fish and crustaceans, as indicated by the presence of animal remains in the stomach and the willingness of crabs to consume fish meat. Laboratory experiments on leaf consumption and leaf preferences of N. versicolor indicate that they preferentially feed on brown leaves, if available, followed by green and yellow leaves. If all species of sesarmid crabs in the Bangrong mangrove forest consume leaves at the same rate as N. versicolor, they could potentially ingest 52% of the total litter fall.

  8. Antimicrobial activity of crude methanolic extracts from Ganoderma lucidum and Trametes versicolor

    Directory of Open Access Journals (Sweden)

    Lukáš Hleba

    2014-11-01

    Full Text Available In this paper the antimicrobial activity of crude methanolic extracts obtained from Ganoderma lucidum and Trametes versicolor were investigated. The antimicrobial activities of the extracts against E. coli, P. aeruginosa, S. epidermis, E. raffinosus, S. cerevisiae and C. albicans were determined by the microbroth dillution method according by EUCAST in 96-well microplates. Microorganisms were obtained from Czech Collection of Microorganisms. Absorbances after and before the experiment were subtracted, converted to binary system and obtained values to Probit analysis were used. Of the two macromycetes extracts tested, not all extracts showed antimicrobial activity in tested MICs range. The highest antimicrobial activity showed the both extracts to Saccharomyces cerevisiae. The less antimicrobial effects had the both macromycetes extracts to Staphylococcus epidermis. Antimicrobial activity of macromycetes methanolic crude extracts to others tested microorganisms showed no effect or used concentration could be higher.

  9. Comparative study of ketoconazole versus selenium sulphide shampoo in pityriasis versicolor

    Directory of Open Access Journals (Sweden)

    Aggarwal K

    2003-03-01

    Full Text Available Forty patients suffering from pityriasis versicolor were treated with either 2% ketoconazole shampoo (20 patients or 2.5% selenium sulphide shampoo (20 patients, once a week for three weeks. On global assessment after one month of start of therapy, 19 (95% out of 20 patients treated with ketoconazole shampoo were cured while one case had mild residual disease. In selenium sulphide shampoo group, 17 (85% out of 20 patients were cured, one had mild residual disease and two had considerable residual disease. No significant difference was observed in the response rates in the two groups. Relapse occurred in one patient of ketoconazole group and two patients of selenium sulphide group during the follow - up period of three months.

  10. Complete mitochondrial genome of the versicoloured emerald hummingbird Amazilia versicolor, a polymorphic species.

    Science.gov (United States)

    Prosdocimi, Francisco; Souto, Helena Magarinos; Ruschi, Piero Angeli; Furtado, Carolina; Jennings, W Bryan

    2016-09-01

    The genome of the versicoloured emerald hummingbird (Amazilia versicolor) was partially sequenced in one-sixth of an Illumina HiSeq lane. The mitochondrial genome was assembled using MIRA and MITObim software, yielding a circular molecule of 16,861 bp in length and deposited in GenBank under the accession number KF624601. The mitogenome contained 13 protein-coding genes, 22 transfer tRNAs, 2 ribosomal RNAs and 1 non-coding control region. The molecule was assembled using 21,927 sequencing reads of 100 bp each, resulting in ∼130 × coverage of uniformly distributed reads along the genome. This is the forth mitochondrial genome described for this highly diverse family of birds and may benefit further phylogenetic, phylogeographic, population genetic and species delimitation studies of hummingbirds. PMID:25758043

  11. Tinea Versicolor

    Science.gov (United States)

    ... Provider May Prescribe References/Trusted Links Related diseases: Post-Inflammatory ... © 2006-2013 Logical Images, Inc. All rights reserved. Advertising Notice This Site and third parties who place ...

  12. Dectin-1 and IL-17A suppress murine asthma induced by Aspergillus versicolor but not Cladosporium cladosporioides due to differences in beta-glucan surface exposure1

    OpenAIRE

    Mintz-Cole, Rachael A.; Gibson, Aaron M.; Bass, Stacey A.; Budelsky, Alison L.; Reponen, Tiina; Hershey, Gurjit K. Khurana

    2012-01-01

    There is considerable evidence supporting a role for mold exposure in the pathogenesis and expression of childhood asthma. Aspergillus versicolor and Cladosporium cladosporioides are common molds that have been implicated in asthma. In a model of mold-induced asthma, mice were repeatedly exposed to either A. versicolor or C. cladosporioides spores. The two molds induced distinct phenotypes and this effect was observed in both Balb/c and C57BL/6 strains. C. cladosporioides induced robust airwa...

  13. Phenylquinolinones with antitumor activity from the Indian Ocean-derived fungus Aspergillus versicolor Y31-2

    Science.gov (United States)

    Li, Peihai; Fan, Yaqin; Chen, Hao; Chao, Yaxi; Du, Ning; Chen, Junhui

    2016-02-01

    Two phenylquinolinones, including one new compound (1) and a previously isolated compound (2), were isolated from the ethyl acetate extracts of the fungus Aspergillus versicolor Y31-2, which was obtained from seawater samples collected from the Indian Ocean. The structures of these compounds were established by spectroscopic analyses. 4-(3-Hydroxyphenyl)-3-methoxyquinolin-2(1H)-one (1) exhibited moderate cytotoxicity against MCF-7 (human breast carcinoma cell line) and SMMC-7721 (human liver cancer cell line) cells with IC50 values of 16.6 and 18.2 μmol/L, respectively. To the best of our knowledge, this study represents the first reported account of the isolation of compounds 1 and 2 as the secondary metabolites of the seawater derived fungus Aspergillus versicolor from the Indian Ocean.

  14. Non conventional biological treatment based on Trametes versicolor for the elimination of recalcitrant anticancer drugs in hospital wastewater

    OpenAIRE

    Ferrando Climent, Laura; Cruz Morató, Carles; Marco Urrea, Ernest; Vicent, Teresa; Sarrà i Adroguer, Montserrat; Rodríguez Mozaz, Sara; Barceló i Cullerés, Damià

    2015-01-01

    This work presents a study about the elimination of anticancer drugs, a group of pollutants considered recalcitrant during conventional activated sludge wastewater treatment, using a biological treatment based on the fungus Trametes versicolor. A 10-L fluidized bed bioreactor inoculated with this fungus was set up in order to evaluate the removal of 10 selected anticancer drugs in real hospital wastewater. Almost all the tested anticancer drugs were completely removed from the wastewater at ...

  15. Walking to survive. Searching, feeding and egg production of the carabid beetle Pterostichus coerulescens L. (= Poecilus versicolor Sturm).

    OpenAIRE

    Mols, P.J.M.

    1993-01-01

    This study concerns the prey-searching and feeding behaviour of the polyphagous groundbeetle Pterostichus coerulescens L. ( = Poecilus versicolor Sturm), a common species on sandy soils. This ground beetle rarely flies, thus preysearching behaviour involves walking. The beetle is diurnal. As object of research, predators of this kind are very suitable because they can be handled easily, their behaviour can be observed directly or filmed with a video camera. Furthermore they are abundantly ava...

  16. Continuous degradation of a mixture of sulfonamides by Trametes versicolor and identification of metabolites from sulfapyridine and sulfathiazole

    International Nuclear Information System (INIS)

    Highlights: ► Degradation of sulfapyridine and sulfathiazole by Trametes versicolor was evaluated. ► The role of laccase and cytochrome P450 was determined. ► Degradation metabolites were identified for sulfapyridine (8) and sulfathiazole (5). ► A mixture of three sulfonamides was degraded in a continuous fluidized bed reactor. - Abstract: In this study, we assessed the degradation of the sulfonamides sulfapyridine (SPY) and sulfathiazole (STZ) by the white-rot fungus Trametes versicolor. Complete degradation was accomplished in fungal cultures at initial pollutant concentrations of approximately 10 mg L−1, although a longer period of time was needed to completely remove STZ in comparison to SPY. When cytochrome P450 inhibitors were added to the fungal cultures, STZ degradation was partially suppressed, while no additional effect was observed for SPY. Experiments with purified laccase and laccase mediators caused the removal of greater than 75% of each antibiotic. Ultra-performance liquid chromatography-quadupole time of flight mass spectrometry (UPLC-QqTOF-MS) analyses allowed the identification of a total of eight degradation intermediates of SPY in both the in vivo and the laccase experiments, being its desulfonated moiety the commonly detected product. For STZ, a total of five products were identified. A fluidized bed reactor with T. versicolor pellets degraded a mixture of sulfonamides (SPY, STZ and sulfamethazine, SMZ) by greater than 94% each at a hydraulic residence time of 72 h. Because wastewater contains many diverse pollutants, these results highlight the potential of T. versicolor as a bioremediation agent not only for the removal of antibiotics but also for the elimination of a wide range of contaminants.

  17. Continuous degradation of a mixture of sulfonamides by Trametes versicolor and identification of metabolites from sulfapyridine and sulfathiazole

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez-Rodriguez, Carlos E., E-mail: CarlosEsteban.Rodriguez@uab.cat [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 2060 San Jose (Costa Rica); Jesus Garcia-Galan, Ma. [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034, Barcelona (Spain); Blanquez, Paqui [Departament d' Enginyeria Quimica, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Diaz-Cruz, M. Silvia [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034, Barcelona (Spain); Barcelo, Damia [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034, Barcelona (Spain); Catalan Institute for Water Research (ICRA), Parc Cientific i Tecnologic de la Universitat de Girona, C/Emili Grahit, 101 Edifici H2O, E-17003 Girona (Spain); Caminal, Gloria [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Vicent, Teresa [Departament d' Enginyeria Quimica, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain)

    2012-04-30

    Highlights: Black-Right-Pointing-Pointer Degradation of sulfapyridine and sulfathiazole by Trametes versicolor was evaluated. Black-Right-Pointing-Pointer The role of laccase and cytochrome P450 was determined. Black-Right-Pointing-Pointer Degradation metabolites were identified for sulfapyridine (8) and sulfathiazole (5). Black-Right-Pointing-Pointer A mixture of three sulfonamides was degraded in a continuous fluidized bed reactor. - Abstract: In this study, we assessed the degradation of the sulfonamides sulfapyridine (SPY) and sulfathiazole (STZ) by the white-rot fungus Trametes versicolor. Complete degradation was accomplished in fungal cultures at initial pollutant concentrations of approximately 10 mg L{sup -1}, although a longer period of time was needed to completely remove STZ in comparison to SPY. When cytochrome P450 inhibitors were added to the fungal cultures, STZ degradation was partially suppressed, while no additional effect was observed for SPY. Experiments with purified laccase and laccase mediators caused the removal of greater than 75% of each antibiotic. Ultra-performance liquid chromatography-quadupole time of flight mass spectrometry (UPLC-QqTOF-MS) analyses allowed the identification of a total of eight degradation intermediates of SPY in both the in vivo and the laccase experiments, being its desulfonated moiety the commonly detected product. For STZ, a total of five products were identified. A fluidized bed reactor with T. versicolor pellets degraded a mixture of sulfonamides (SPY, STZ and sulfamethazine, SMZ) by greater than 94% each at a hydraulic residence time of 72 h. Because wastewater contains many diverse pollutants, these results highlight the potential of T. versicolor as a bioremediation agent not only for the removal of antibiotics but also for the elimination of a wide range of contaminants.

  18. Lignin Peroxidase Activity Is Not Important in Biological Bleaching and Delignification of Unbleached Kraft Pulp by Trametes versicolor

    OpenAIRE

    Archibald, Frederick S.

    1992-01-01

    The discovery in 1983 of fungal lignin peroxidases able to catalyze the oxidation of nonphenolic aromatic lignin model compounds and release some CO2 from lignin has been seen as a major advance in understanding how fungi degrade lignin. Recently, the fungus Trametes versicolor was shown to be capable of substantial decolorization and delignification of unbleached industrial kraft pulps over 2 to 5 days. The role, if any, of lignin peroxidase in this biobleaching was therefore examined. Sever...

  19. Removal of pharmaceuticals, polybrominated flame retardants and UV-filters from sludge by the fungus Trametes versicolor in bioslurry reactor

    International Nuclear Information System (INIS)

    Highlights: ► Sludge from a WWTP was treated in a fungal slurry reactor with Trametes versicolor. ► Twenty-four pharmaceuticals were removed at important extents. ► UV-filters and brominated flame retardants were also degraded. ► Overall toxicity of sludge increased despite the pollutant removal. - Abstract: Conventional wastewater treatments are inefficient in the removal of many organic pollutants. The presence of these contaminants in the final sludge represents a source of environmental pollution due to the increasing use of biosolids in land application. A biotechnological approach which employed the fungus Trametes versicolor in a sludge-bioslurry reactor was assessed in order to remove several groups of emerging pollutants. Biological fungal activity was monitored by means of ergosterol and laccase determinations. Fifteen out of 24 detected pharmaceuticals were removed at efficiencies over 50% after the treatment, including eight completely degraded. Removal ranged between 16–53% and 22–100% for the brominated flame retardants and the UV-filters, respectively. Only two of all the detected compounds remained unchanged after the treatment. Although elimination results are promising, the toxicity of the final sludge increased after the treatment. This finding is contrary to the toxicity results obtained in similar treatments of sludge with T. versicolor in solid-phase.

  20. Degradation of the drug sodium diclofenac by Trametes versicolor pellets and identification of some intermediates by NMR

    Energy Technology Data Exchange (ETDEWEB)

    Marco-Urrea, Ernest [Departament d' Enginyeria Quimica and Institut de Ciencia i Tecnologia Ambiental, Escola d' Enginyeria (Estonia), Universitat Autonoma de Barcelona (UAB), 08193 Bellaterra (Spain); Perez-Trujillo, Miriam [Servei de Ressonancia Magnetica Nuclear, UAB, 08193 Bellaterra (Spain); Cruz-Morato, Carles [Departament d' Enginyeria Quimica and Institut de Ciencia i Tecnologia Ambiental, Escola d' Enginyeria (Estonia), Universitat Autonoma de Barcelona (UAB), 08193 Bellaterra (Spain); Caminal, Gloria, E-mail: gloria.caminal@uab.es [Unitat de Biocatalisis Aplicada associada al IQAC (CSIC-UAB), EE, UAB, 08193 Bellaterra (Spain); Vicent, Teresa [Departament d' Enginyeria Quimica and Institut de Ciencia i Tecnologia Ambiental, Escola d' Enginyeria (Estonia), Universitat Autonoma de Barcelona (UAB), 08193 Bellaterra (Spain)

    2010-04-15

    Degradation of diclofenac sodium, a nonsteroidal anti-inflammatory drug widely found in the aquatic environment, was assessed using the white-rot fungus Trametes versicolor. Almost complete diclofenac removal ({>=}94%) occurred the first hour with T. versicolor pellets when the drug was added at relatively high (10 mg L{sup -1}) and environmentally relevant low (45 {mu}g L{sup -1}) concentrations in a defined liquid medium. In vivo and in vitro experiments using the cytochrome P450 inhibitor 1-aminobenzotriazole and purified laccase, respectively, suggested at least two different mechanisms employed by T. versicolor to initiate diclofenac degradation. Two hydroxylated metabolites, 4'-hydroxydiclofenac and 5-hydroxydiclofenac, were structurally elucidated by nuclear magnetic resonance as degradation intermediates in fungal cultures spiked with diclofenac. Both parent compound and intermediates disappeared after 24 h leading to a decrease in ecotoxicity calculated by the Microtox test. Laccase-catalyzed transformation of diclofenac led to the formation of 4-(2,6-dichlorophenylamino)-1,3-benzenedimethanol, which was not detected in in vivo experiments probably due to the low laccase activity levels observed through the first hours of incubation.

  1. Degradation of the drug sodium diclofenac by Trametes versicolor pellets and identification of some intermediates by NMR

    International Nuclear Information System (INIS)

    Degradation of diclofenac sodium, a nonsteroidal anti-inflammatory drug widely found in the aquatic environment, was assessed using the white-rot fungus Trametes versicolor. Almost complete diclofenac removal (≥94%) occurred the first hour with T. versicolor pellets when the drug was added at relatively high (10 mg L-1) and environmentally relevant low (45 μg L-1) concentrations in a defined liquid medium. In vivo and in vitro experiments using the cytochrome P450 inhibitor 1-aminobenzotriazole and purified laccase, respectively, suggested at least two different mechanisms employed by T. versicolor to initiate diclofenac degradation. Two hydroxylated metabolites, 4'-hydroxydiclofenac and 5-hydroxydiclofenac, were structurally elucidated by nuclear magnetic resonance as degradation intermediates in fungal cultures spiked with diclofenac. Both parent compound and intermediates disappeared after 24 h leading to a decrease in ecotoxicity calculated by the Microtox test. Laccase-catalyzed transformation of diclofenac led to the formation of 4-(2,6-dichlorophenylamino)-1,3-benzenedimethanol, which was not detected in in vivo experiments probably due to the low laccase activity levels observed through the first hours of incubation.

  2. Removal of pharmaceuticals, polybrominated flame retardants and UV-filters from sludge by the fungus Trametes versicolor in bioslurry reactor

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez-Rodriguez, Carlos E. [Unitat Asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 2060 San Jose (Costa Rica); Baron, Enrique; Gago-Ferrero, Pablo; Jelic, Aleksandra; Llorca, Marta; Farre, Marinella; Diaz-Cruz, M. Silvia; Eljarrat, Ethel [Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona (Spain); Petrovic, Mira [Institucio Catalana de Reserca i Estudis Avancats (ICREA), Passeig Lluis Companys 23, 80010 Barcelona (Spain); Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona (Spain); Caminal, Gloria, E-mail: Gloria.Caminal@uab.cat [Unitat Asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Barcelo, Damia [Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona (Spain); Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona (Spain); King Saud University (KSU), P.O. Box 2455, 11451 Riyadh (Saudi Arabia); and others

    2012-09-30

    Highlights: Black-Right-Pointing-Pointer Sludge from a WWTP was treated in a fungal slurry reactor with Trametes versicolor. Black-Right-Pointing-Pointer Twenty-four pharmaceuticals were removed at important extents. Black-Right-Pointing-Pointer UV-filters and brominated flame retardants were also degraded. Black-Right-Pointing-Pointer Overall toxicity of sludge increased despite the pollutant removal. - Abstract: Conventional wastewater treatments are inefficient in the removal of many organic pollutants. The presence of these contaminants in the final sludge represents a source of environmental pollution due to the increasing use of biosolids in land application. A biotechnological approach which employed the fungus Trametes versicolor in a sludge-bioslurry reactor was assessed in order to remove several groups of emerging pollutants. Biological fungal activity was monitored by means of ergosterol and laccase determinations. Fifteen out of 24 detected pharmaceuticals were removed at efficiencies over 50% after the treatment, including eight completely degraded. Removal ranged between 16-53% and 22-100% for the brominated flame retardants and the UV-filters, respectively. Only two of all the detected compounds remained unchanged after the treatment. Although elimination results are promising, the toxicity of the final sludge increased after the treatment. This finding is contrary to the toxicity results obtained in similar treatments of sludge with T. versicolor in solid-phase.

  3. Adsorption of Trametes versicolor laccase to soil iron and aluminum minerals: enzyme activity, kinetics and stability studies.

    Science.gov (United States)

    Wu, Yue; Jiang, Ying; Jiao, Jiaguo; Liu, Manqiang; Hu, Feng; Griffiths, Bryan S; Li, Huixin

    2014-02-01

    Laccases play an important role in the degradation of soil phenol or phenol-like substance and can be potentially used in soil remediation through immobilization. Iron and aluminum minerals can adsorb extracellular enzymes in soil environment. In the present study, we investigated the adsorptive interaction of laccase, from the white-rot fungus Trametes versicolor, with soil iron and aluminum minerals and characterized the properties of the enzyme after adsorption to minerals. Results showed that both soil iron and aluminum minerals adsorbed great amount of laccase, independent of the mineral specific surface areas. Adsorbed laccases retained 26-64% of the activity of the free enzyme. Compared to the free laccase, all adsorbed laccases showed higher Km values and lower Vmax values, indicating a reduced enzyme-substrate affinity and a lower rate of substrate conversion in reactions catalyzed by the adsorbed laccase. Adsorbed laccases exhibited increased catalytic activities compared to the free laccase at low pH, implying the suitable application of iron and aluminum mineral-adsorbed T. versicolor laccase in soil bioremediation, especially in acid soils. In terms of the thermal profiles, adsorbed laccases showed decreased thermal stability and higher temperature sensitivity relative to the free laccase. Moreover, adsorption improved the resistance of laccase to proteolysis and extended the lifespan of laccase. Our results implied that adsorbed T. versicolor laccase on soil iron and aluminum minerals had promising potential in soil remediation. PMID:24225344

  4. Host plant preference in Colorado potato beetle (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Field and laboratory-choice tests were conducted to better understand host plant preference by the Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), in Virginia. In laboratory olfactometer studies, L. decemlineata preferred potato over both tomato and eggplant foli...

  5. Interaction between perchlorate and iodine in the metamorphosis of Hyla versicolor

    Science.gov (United States)

    Sparling, D.W.; Harvey, G.; Nzengung, V.

    2003-01-01

    Perchlorate (ClO4-) is a water-soluble, inorganic anion that is often combined with ammonium, potassium or other cations for use in industry and agriculture. Ammonium perchlorate, for example, is a potent oxidizer and is used in various military applications including rocket fuel. It has also been found in an historically widely used fertilizer, Chilean nitrate and in other fertilizers. It has been found in ground and surface waters of over 30 states and is considered a human health risk. Because of its similar atomic radius and volume, perchlorate competes with iodide for thyroid uptake and storage and thereby inhibits production of thyroid hormones. Amphibians may be particularly affected by perchlorate because they rely on the thyroid for metamorphosis. This study exposed early larval Hyla versicolor to concentrations of perchlorate ranging from 2.2 to 50 ppm to determine the effects of perchlorate on a native amphibian. In addition, three controls, 0 perchlorate, 0 perchlorate with 0.10 ppm iodide (C + I) and 50 ppm perchlorate + 0.10 ppm iodide (50 + I) were tested. Mortality (metamorphosis. The estimated EC50 for total inhibition of metamorphosis at 70 days of treatment was 3.63 ppm. There was no evidence of inhibition of development with the 50 + I, C + I, or controls, indicating that the presence of small concentrations of iodide could counter the effects of perchlorate. When tadpoles that had been inhibited by perchlorate were subsequently treated with iodide, development through prometamorphosis progressed but mortality was very high.

  6. Phase 1 Clinical Trial of Trametes versicolor in Women with Breast Cancer.

    Science.gov (United States)

    Torkelson, Carolyn J; Sweet, Erin; Martzen, Mark R; Sasagawa, Masa; Wenner, Cynthia A; Gay, Juliette; Putiri, Amy; Standish, Leanna J

    2012-01-01

    Introduction. Orally administered preparations from the Trametes versicolor (Tv) mushroom have been hypothesized to improve immune response in women with breast cancer after standard chemotherapy and radiotherapy. Methods. A phase I, two-center, dose escalation study was done to determine the maximum tolerated dose of a Tv preparation when taken daily in divided doses for 6 weeks after recent completion of radiotherapy. Eleven participants were recruited and nine women completed the study. Each cohort was comprised of three participants given one of three doses of Tv (3, 6, or 9 grams). Immune data was collected pre- and postradiation, at 3 on-treatment time points and after a 3-week washout. Results. Nine adverse events were reported (7 mild, 1 moderate, and 1 severe), suggesting that Tv was well tolerated. Immunological results indicated trends in (1) increased lymphocyte counts at 6 and 9 grams/day; (2) increased natural killer cell functional activity at 6 grams/day; (3) dose-related increases in CD8(+) T cells and CD19(+) B cells , but not CD4(+) T cells or CD16(+)56(+) NK cells. Conclusion. These findings show that up to 9 grams/day of a Tv preparation is safe and tolerable in women with breast cancer in the postprimary treatment setting. This Tv preparation may improve immune status in immunocompromised breast cancer patients following standard primary oncologic treatment. PMID:22701186

  7. Treatment of a textile effluent from dyeing with cochineal extracts using Trametes versicolor fungus.

    Science.gov (United States)

    Arroyo-Figueroa, Gabriela; Ruiz-Aguilar, Graciela M L; López-Martínez, Leticia; González-Sánchez, Guillermo; Cuevas-Rodríguez, Germán; Rodríguez-Vázquez, Refugio

    2011-01-01

    Trametes versicolor (Tv) fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. However, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. Experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of Tv fungus were done. Treatments to determine decolorization in the presence or absence of Kirk's medium, glucose, and fungus, with an addition of 50% (v v-1) of nonsterilized effluent were performed. Physicochemical characterization was performed at the start and end of the treatment. Degradation kinetics were determined. A direct relationship was found between the dry weight of fungi, pH, and the decolorization system, with higher decolorization at lower pH levels (pH ~4.3). High decolorization (81% ± 0.09; 88% ± 0.17; and 99% ± 0.04) for three of the eight treatments (Kirk's medium without glucose, Kirk's medium with glucose, and without medium with glucose, respectively) was found. Toxicity tests determined an increase in the initial effluent toxicity (7.33 TU) compared with the final treatment (47.73 TU) in a period of 11 days. For this system, a degradation sequence of the carminic acid structure present in the effluent by the Tv fungus is suggested, in which it is seen that metabolites still containing aromatic structures are generated. PMID:21552764

  8. Treatment of a Textile Effluent from Dyeing with Cochineal Extracts Using Trametes versicolor Fungus

    Directory of Open Access Journals (Sweden)

    Gabriela Arroyo-Figueroa

    2011-01-01

    Full Text Available Trametes versicolor (Tv fungus can degrade synthetic dyes that contain azo groups, anthraquinone, triphenylmethane polymers, and heterocyclic groups. However, no references have been found related to the degradation of natural dyes, such as the carminic acid that is contained in the cochineal extract. Experiments to determine the decolorization of the effluent used in the cotton dyeing process with cochineal extract by means of Tv fungus were done. Treatments to determine decolorization in the presence or absence of Kirk's medium, glucose, and fungus, with an addition of 50% (v v-1 of nonsterilized effluent were performed. Physicochemical characterization was performed at the start and end of the treatment. Degradation kinetics were determined. A direct relationship was found between the dry weight of fungi, pH, and the decolorization system, with higher decolorization at lower pH levels (pH ~4.3. High decolorization (81% ± 0.09; 88% ± 0.17; and 99% ± 0.04 for three of the eight treatments (Kirk's medium without glucose, Kirk's medium with glucose, and without medium with glucose, respectively was found. Toxicity tests determined an increase in the initial effluent toxicity (7.33 TU compared with the final treatment (47.73 TU in a period of 11 days. For this system, a degradation sequence of the carminic acid structure present in the effluent by the Tv fungus is suggested, in which it is seen that metabolites still containing aromatic structures are generated.

  9. A multivariate analysis of genetic variation in the advertisement call of the gray treefrog, Hyla versicolor.

    Science.gov (United States)

    Welch, Allison M; Smith, Michael J; Gerhardt, H Carl

    2014-06-01

    Genetic variation in sexual displays is crucial for an evolutionary response to sexual selection, but can be eroded by strong selection. Identifying the magnitude and sources of additive genetic variance underlying sexually selected traits is thus an important issue in evolutionary biology. We conducted a quantitative genetics experiment with gray treefrogs (Hyla versicolor) to investigate genetic variances and covariances among features of the male advertisement call. Two energetically expensive traits showed significant genetic variation: call duration, expressed as number of pulses per call, and call rate, represented by its inverse, call period. These two properties also showed significant genetic covariance, consistent with an energetic constraint to call production. Combining the genetic variance-covariance matrix with previous estimates of directional sexual selection imposed by female preferences predicts a limited increase in call duration but no change in call rate despite significant selection on both traits. In addition to constraints imposed by the genetic covariance structure, an evolutionary response to sexual selection may also be limited by high energetic costs of long-duration calls and by preferences that act most strongly against very short-duration calls. Meanwhile, the persistence of these preferences could be explained by costs of mating with males with especially unattractive calls. PMID:24621402

  10. Efficient removal of disperse dye by mixed culture of ganoderma lucidum and coriolus versicolor

    International Nuclear Information System (INIS)

    In the current study, an attempt was made to check the potential of aerobic mixed culture of two indigenous white rot fungi for the decolorization of different disperse dyes in batch culture mode and optimization of different conditions to enhance the biotransformation of dyes. Initial screening trial with six disperse dyes, viz. (Foron Yellow RD5GL, Foron Red RDRBLS, Foron Rubine RDGFL, Foron Black RD3GRN, Foron Blue RDGLN and Foron Turquoise SBLN), was carried out using mixed culture of Ganoderma lucidum and Coriolus versicolor. From all the tested dyes, the mixed culture showed better removal efficiency (93 %) with Foron Turquoise SBLN dye after 8 days of incubation period as compared to other tested dyes. Enhanced color removal (98 %) was observed when the medium was amended by ammonium tartarate, maltose, MnSO/sub 4/ at pH 4.5 and 30 degree C with 2 mL fungal culture during 2nd day of incubation period. Enzyme profile showed that the mixed culture produced three liginolytic enzymes like lignin peroxidase (LiP), manganase peroxidase (MnP) and laccase but MnP was found to be the major enzyme. The results indicated that white rot fungi (WRF) could be used to treat wastewater containing disperse dyes. (author)

  11. Setting the stage for electron transfer: Molecular basis of ABTS-binding to four laccases from Trametes versicolor at variable pH and protein oxidation state

    DEFF Research Database (Denmark)

    Christensen, Niels Johan; Kepp, Kasper Planeta

    2014-01-01

    and activity for unknown reasons, and are thus useful probes of stability-function trade-offs relevant to protein engineering. We report here the first systematic comparison of ABTS-binding to different proteins, i.e. the four isoforms of Trametes versicolor, using a combination of sequence clustering...... the four T. versicolor isoforms belonging to separate classes. The T1 oxidation state had minor effect on ABTS binding, whereas the protonation state of Asp206 was important, consistent with site-directed mutagenesis studies. The absence of active poses for the δ-isoform agrees with its large Km...

  12. Evaluation of pityriasis versicolor in prisoners: A cross-sectional study

    Directory of Open Access Journals (Sweden)

    Salahi-Moghaddam Abdoreza

    2009-01-01

    Full Text Available Background: Pityriasis versicolor (PV is a mild but recurrent superficial fungal infection of stratum corneum. Many predisposing factors such as late teen and young adulthood age, tropical and subtropical climate (and to a lesser extent temperate climate, immunosuppression, malnutrition, use of oral contraceptives, hyperhidrosis, poor hygiene and a few other factors have been proposed for this condition. Aim: Regarding these probable and possible risk factors, we felt that jail is a unique population which can be affected by this infection and therefore we decided to evaluate some aspects of this infection in this population. Methods: In this cross-sectional study in male prisoners of Bandarabbas city, 230 prisoners were randomly selected. One hundred and five (45.67% of them were diagnosed to be infected. They were also evaluated regarding age, duration of being imprisoned, skin color, site of lesion and its pigmentation change and any concomitant clinical manifestation such as pruritis, burning or cebure. Chi-square test or Fisher′s exact test was used for data analysis. Results: Mean age of infected population was 31.62 ± 8.67 years while peak incidence of PV was in 28-32 years age group. The most common color change was hypopigmentation. No case of PV hyperpigmentation was seen. We could not also observe any association between skin color and duration of being imprisoned with the frequency of infection. Conclusion: There seems to be no difference between pattern of infection in the general population and prisoners′ population in the aspects evaluated.

  13. Influence of treatment conditions on the oxidation of micropollutants by Trametes versicolor laccase.

    Science.gov (United States)

    Margot, Jonas; Maillard, Julien; Rossi, Luca; Barry, D A; Holliger, Christof

    2013-09-25

    Many organic compounds present at low concentrations in municipal wastewater, such as various pharmaceuticals and biocides, are recalcitrant in conventional wastewater treatment plants (WWTPs). To improve their biodegradation, oxidoreductase enzymes such as laccases were tested. The goal was to find optimal conditions for the transformation of two anti-inflammatory pharmaceuticals (diclofenac (DFC) and mefenamic acid (MFA)), one biocide (triclosan (TCN)) and one plastic additive (bisphenol A (BPA)) by Trametes versicolor laccase. Experiments were conducted in spiked solutions at different pH values (from 3 to 9), enzyme concentrations (70-1400 Ul(-1)), reaction times (0-26 hours) and temperatures (10, 25 and 40°C) following a Doehlert experimental design. A semi-empirical model was developed to understand better the combined effects of the four factors and to determine optimal values. This model was able to fit well the experimental data (R(2)>0.97) and showed good predictive ability. All four factors had a significant effect on the micropollutant oxidation with the greatest influence shown by pH. Results for single compounds were different from those obtained for mixtures of micropollutants. For instance, DFC transformation occurred at much higher rates in mixtures under alkaline conditions. Optimal conditions were compound-dependent, but were found to be between pH 4.5 to 6.5 and between 25°C to more than 40°C. A laccase concentration of 730 Ul(-1) was sufficient to obtain a high removal rate (>90%) of the four individual compounds (range of times: 40 min to 5 hours), showing the potential of laccases to improve biodegradation of environmentally persistent compounds. PMID:23831273

  14. High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

    Science.gov (United States)

    Ottoni, Cristiane; Simões, Marta F.; Fernandes, Sara; Santos, Cledir R.; Lima, Nelson

    2016-01-01

    Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U∙L−1) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs. PMID:27490563

  15. KINETIC CHARACTERIZATION OF PURIFIED LACCASE PRODUCED FROM Trametes versicolor IBL-04 IN SOLID STATE BIO-PROCESSING OF CORNCOBS

    Directory of Open Access Journals (Sweden)

    Muhammad Asgher,

    2012-01-01

    Full Text Available A locally isolated white rot fungal strain Trametes versicolor IBL-04 produced high laccase activities in solid state bio-processing of corn cobs. Addition of glucose and yeast extract (C: N ratio; 25:1 enhanced laccase synthesis. Addition of Tween-80 and CuSO4 enhanced laccase production to 1012 U/mL under optimized process conditions. Laccase was further purified to 2.89-fold (specific activity of 840 U/mg by ammonium sulfate fractional precipitation, dialysis, and Sephadex G-100 gel filtration chromatography. The purified laccase had a relative molecular mass of 63 kDa as detected by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE. Best enzyme activity was at pH 5 and 40oC. Using 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS as substrate, the enzyme showed maximum activity (Vmax of 780 U/mL with a corresponding Michaelis constant (Km value of 73µM. Among the different activators/inhibitors, Cu2+, Mn2+, and Fe2+ stimulated laccase activity, whereas EDTA and cystein inhibited the enzyme. The higher Vmax and lower Km for T. versicolor IBL-04 laccase as compared to most of the reported laccases suggests its potential for industrial applications.

  16. Expression of phenotypic plasticity in hatchlings of the lizard Calotes versicolor (Squamata: Agamidae: influence of nest moisture

    Directory of Open Access Journals (Sweden)

    Bhagyashri A. Shanbhag

    2012-06-01

    Full Text Available Calotes versicolor breed from late May to early October. The breeding activity begins with the onset of the southwest monsoon. The eggs laid in early breeding season experience more wet conditions than those of the late breeding season. We studied the influence of nest moisture levels on the phenotypic traits of hatchlings by burying the eggs in 5-cm-deep sand nests with ~50%(wet nest or ~20% (relatively dry nest moisture to simulate nesting conditions of early and late breeding seasons. A group of eggs were subjected to standard laboratory incubation procedure in which eggs are half-buried in the moist sand and the other half exposed to air. Hatching time and hatchling body size varied with the treatment. The eggs from dry nests hatched later and hatchlings were the biggest but possessed least amount of residual yolk compared to those of 'wet nest' and also 'lab incubated' groups. In these hatchlings head and limb sizes were significantly larger than that of the other two groups. The findings show: (1 a developmental plasticity in the lizard, (2 that under low moist conditionslarger body size is preferred, and (3 that the trade-off between somatic growth of embryos and future energy reserves (residual yolk of hatchlings is influenced by the soil moisture in C. versicolor.

  17. Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolor FISCH.

    Science.gov (United States)

    Obmann, Astrid; Zehl, Martin; Purevsuren, Sodnomtseren; Narantuya, Samdan; Reznicek, Gottfried; Kletter, Christa; Glasl, Sabine

    2011-02-01

    An HPLC-diode array detection (DAD) method was established in order to investigate dried aerial parts of Dianthus versicolor FISCH. (Caryophyllaceae), a plant used in traditional Mongolian medicine against liver impairment. Aqueous extracts were separated on an Aquasil(®) C(18) column with a linear gradient of acetonitrile (ACN) and water (adjusted to pH 2.8 with formic acid) as the mobile phase. LC-IT-MS facilitated the assignment of 26 flavonoids, among them a series of rare C-glycosylated as well as O-glycosylated derivatives, which are assumed to be the active principles. Quantification was performed and validated using isovitexin-7-O-glucoside (saponarin) as the external standard. The method showed good linear behaviour (r(2) ≥0.9999) over the investigated concentration range (0.007-3.5 mg/mL). The good precision of the method allowed the successful qualitative and quantitative analysis of flavonoid-glycosides in the aqueous extracts prepared from five different D. versicolor samples. Depending on the origin of the samples, the total flavonoid content was found to vary considerably from 0.41 to 3.30% in the aqueous extracts and from 0.07 to 0.57% in the crude drug. In addition, the relative composition of the various flavonoids was found to differ strongly. These results highlight the need for proper quality control for this herbal drug. PMID:21268252

  18. High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs.

    Science.gov (United States)

    Ottoni, Cristiane; Simões, Marta F; Fernandes, Sara; Santos, Cledir R; Lima, Nelson

    2016-01-01

    Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U∙L(-1)) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs. PMID:27490563

  19. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor

    NARCIS (Netherlands)

    Kozarski, M.; Klaus, A.; Niksic, M.; Vrvic, M.M.; Todorovic, N.; Jakovljevic, D.; Griensven, van L.J.L.D.

    2012-01-01

    Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts wer

  20. Isolation, structure elucidation, and biomimetic total synthesis of versicolamide B and the isolation of antipodal (-)-stephacidin A and (+)-notoamide B from Aspergillus versicolor

    Science.gov (United States)

    A new prenylated indole alkaloid, versicolamide B, was isolated from cultures of Aspergillus versicolor NRRL 35600. The structure was assigned by 2D NMR data, and confirmed by a biomimetic total synthesis. Versicolamide B is the first member of the paraherquamide-stephacidin family of alkaloids fo...

  1. Molecular markers detect cryptic predation on coffee berry borer (Coleoptera: Curculionidae) by silvanid and laemophloeid flat bark beetles (Coleoptera: Silvanidae, Laemophloeidae) in coffee beans

    Science.gov (United States)

    The coffee berry borer, Hypothenemus hampei(Coleoptera: Curculionidae)(Ferrari), is a serious pest of coffee worldwide and has been recently introduced in Hawai’i, first detected in the state in 2010. Adult silvanid flat bark beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and adult laemoph...

  2. Clinical analysis of Tinea versicolor in 50 children%花斑癣50例临床分析

    Institute of Scientific and Technical Information of China (English)

    魏文

    2009-01-01

    目的 探讨儿童花斑癣的临床特征、治疗效果以及转归.方法 对50例儿童花斑癣患儿的临床资料、治疗及转归进行回顾性分析.结果 患儿面部皮损多见,夏秋季好发,乡镇儿童患病率高,发病年龄2月~14岁,平均2.3岁.男女发病率之比1.6:1.50例患儿均采用联苯苄唑治疗,有25例患儿联合特比萘芬乳膏合用治疗.50例患儿均治愈.结论 儿童花斑癣是一种少见的疾病,其皮损好发面部,与季节、环境及卫生习惯、家族易感性均密切相关.联苯苄唑是治疗儿童花斑癣首选药物,联合特比萘芬治疗有协同作用.%Objective To investigate the clinical features, therapeutic effect and prognosis of tinea versicolor in children. Methods Diagnosis, treatment and prognosis data of clinical tinea versicolor cases in 50children's were analyzed retrospectively. Results The facial skin in children had high prealence in summer, in villages and towns; children aged from 2 months to 14 years old, the average age was 2.3.The incidence rate of men and women was 1.6:1. 50 cases were treated by bifonazole cream on lesions,25 cases were treated with bifonazole cream and terbinafine cream on lesions. 50 cases of patients were cured. Conclusions Tinea versicolor in children is a rare disease, easy to occur in face, correlated with the season, environmental and health habits and family susceptibility. Bifonazole is choice for treatment of childhood tinea versieolor, terbinafine synergies is jointed treatment.

  3. Secondary metabolites of a deep sea derived fungus Aspergillus versicolor CXCTD-06-6a and their bioactivity

    Science.gov (United States)

    Kong, Xianglan; Cai, Shengxin; Zhu, Tianjiao; Gu, Qianqun; Li, Dehai; Luan, Yepeng

    2014-08-01

    In order to obtain novel secondary metabolites, a deep sea inhabiting fungus Aspergillus versicolor CXCTD-06-6a was investigated. One new diketopiperazine brevianamide W ( 1a), as well as five known diketopiperazine alkaloids, diketopiperazine V ( 1b), brevianamide Q ( 2), brevianamide R ( 3), brevianamide K ( 4), and brevianamide E ( 5), were isolated from the EtOAc extract of the fermentation broth. Their structures were elucidated by spectroscopy techniques (NMR, MS). The six compounds exhibited moderate radical scavenging activity against DPPH with clearance ratio of 55.0% ( 1a and 1b), 53.7% ( 2), 46.2% ( 3), 61.4% ( 4) and 19.3% ( 5) at a concentration of 13.9 μmol L-1, respectively; while the positive control ascorbic acid showed a ratio of 70.3% at the concentration of 28.4 μmol L-1.

  4. Historical isolation versus recent long-distance connections between Europe and Africa in bifid toadflaxes (Linaria sect. Versicolores.

    Directory of Open Access Journals (Sweden)

    Mario Fernández-Mazuecos

    Full Text Available BACKGROUND: Due to its complex, dynamic and well-known paleogeography, the Mediterranean region provides an ideal framework to study the colonization history of plant lineages. The genus Linaria has its diversity centre in the Mediterranean region, both in Europe and Africa. The last land connection between both continental plates occurred during the Messinian Salinity Crisis, in the late Miocene (5.96 to 5.33 Ma. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed the colonization history of Linaria sect. Versicolores (bifid toadflaxes, which includes c. 22 species distributed across the Mediterranean, including Europe and Africa. Two cpDNA regions (rpl32-trnL(UAG and trnK-matK were sequenced from 66 samples of Linaria. We conducted phylogenetic, dating, biogeographic and phylogeographic analyses to reconstruct colonization patterns in space and time. Four major clades were found: two of them exclusively contain Iberian samples, while the other two include northern African samples together with some European samples. The bifid toadflaxes have been split in African and European clades since the late Miocene, and most lineage and speciation differentiation occurred during the Pliocene and Quaternary. We have strongly inferred four events of post-Messinian colonization following long-distance dispersal from northern Africa to the Iberian Peninsula, Sicily and Greece. CONCLUSIONS/SIGNIFICANCE: The current distribution of Linaria sect. Versicolores lineages is explained by both ancient isolation between African and European populations and recent events of long-distance dispersal over sea barriers. This result provides new evidence for the biogeographic complexity of the Mediterranean region.

  5. Enzymatic removal of estrogenic activity of nonylphenol and octylphenol aqueous solutions by immobilized laccase from Trametes versicolor

    Energy Technology Data Exchange (ETDEWEB)

    Catapane, Maria [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Nicolucci, Carla; Menale, Ciro; Mita, Luigi [National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138 Naples (Italy); Rossi, Sergio [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); Mita, Damiano G., E-mail: mita@igb.cnr.it [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138 Naples (Italy); Diano, Nadia [Institute of Genetics and Biophysics “ABT”, Via P. Castellino, 111, 80131 Naples (Italy); National Institute of Biostructures and Biosystems (INBB), Viale Medaglie d’Oro, 305, 00136 Rome (Italy); Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli, 16, 80138 Naples (Italy)

    2013-03-15

    Highlights: ► Endocrine disruptors cause adverse effects in living organisms. ► Nonylphenol and Octylphenol are alkylphenols recognized as endocrine disruptors. ► It is necessary to remove or reduce their presence in the environment. ► Waters polluted by these pollutants have been bioremediated by immobilized laccase from Trametes versicolor. ► Laccase treated solutions were found to have lost any estrogenic activity. -- Abstract: A fluidized bed reactor, filled with laccase-based beads, has been employed to bioremediate aqueous solutions polluted by endocrine disruptors belonging to the alkylphenols (APs) class. In particular Octylphenol and Nonylphenol have been studied. The catalytic activity of free and immobilized laccase from Trametes versicolor has been characterized as a function of pH, temperature and substrate concentration in the reaction medium. In view of practical applications for each substrate concentration the removal efficiency (RE), the time to halve the initial concentration (τ{sub 50}), and the t{sub c=0}, i.e. the time to reach complete pollutant removal, have been calculated. The immobilized laccase exhibited a lower affinity for octylphenol (K{sub m} = 1.11 mM) than for Nonylphenol (K{sub m} = 0.72 mM), but all the other parameters of applicative interest resulted more significant for octylphenol. For example, the times to reach the complete removal of octylphenol compared to those for nonylphenol at the same concentration is shorter of about 15% (at low concentrations) up to 40% (at high concentrations). The study of cell proliferation with MPP89 cells, a human mesothelioma cell line, and the assay with the YES test indicated the loss of estrogenic activity of the APs solutions after laccase treatment.

  6. Intoxicação experimental por Simarouba versicolor (Simaroubaceae em ovinos e indução de resistência ao consumo da planta

    Directory of Open Access Journals (Sweden)

    Ariany C. Santos

    2013-03-01

    Full Text Available Simarouba versicolor é uma árvore semidecídua pertencente à família Simaroubaceae. Um surto de intoxicação por S. versicolor em bovinos por brotos da planta presente no pasto em Mato Grosso do Sul e sua reprodução experimental foram descritos. Esse estudo teve por objetivos verificar experimentalmente se os ovinos podem ser utilizados como modelo clínico-patológico no estudo da intoxicação por Simarouba versicolor St. Hil. (fam. Simaroubaceae, determinar se há indução de resistência pela ingestão de pequenas e repetidas doses e, se a planta mantém sua toxicidade quando dessecada. Foram realizados dois experimentos, sendo o primeiro com folhas verdes ou folhas dessecadas e trituradas de S. versicolor em doses únicas de 5g/kg, 5g/kg e 3g/kg a três ovinos (Ovino 1, 2 e 3 respectivamente. O experimento 2, foi realizado com diferentes doses diárias de folhas dessecadas e trituradas de S. versicolor em quatro ovinos que receberam 1,5g/kg, 0,75g/kg, 0,6g/kg e 0,3g/kg e, com um ovino que recebeu 3g/kg como controle positivo (Ovino 4. A administração foi suspensa quando os ovinos apresentaram sinais clínicos da intoxicação. Após doze dias de recuperação, os animais sobreviventes foram desafiados com a mesma dose diária da planta ingerida anteriormente para avaliar o desenvolvimento de resistência. Os sinais clínicos observados nos dois experimentos caracterizaram-se por anorexia, mucosas oculares congestas, polidipsia, sialorreia, fezes pastosas que evoluíram para diarreia líquida fétida esverdeada, decúbito lateral e morte para os Ovinos 1 a 7. As principais lesões histológicas observadas foram necrose do tecido linfoide (linfonodos, baço, placas de Peyer e enterite necrosante. Com os resultados obtidos, pode-se concluir que os ovinos podem ser utilizados como modelo experimental clínico-patológico na intoxicação por S. versicolor. Com o método utilizado, não houve resistência ao consumo diário de folhas

  7. Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera)

    NARCIS (Netherlands)

    Alexander, K.

    2003-01-01

    Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera) Data are presented on the distribution of selected species from two coleopteran families chosen to represent a random slice of the British fauna. The species have been chosen as exhibiting extremes of range chang

  8. Noteworthy records of Hispines from Belize (Coleoptera: Chrysomelidae)

    OpenAIRE

    Naczi, Robert F. C.; Charles L. Staines

    2011-01-01

    Cephaloleia consanguinea Baly, Cephaloleia fulvolimbata Baly, Cephaloleia ruficollis Baly, Chalepus amabilis Baly, Chalepus brevicornis (Baly), Chalepus pici Descarpentries and Villiers, Microrhopala erebus (Newman), Octhispa bimaculata Uhmann, Octotoma championi Baly, Pseudispa tuberculata Staines, Sceloenopla erudita (Baly), Stenispa guatemalensis Uhmann, Sumitrosis gestroi (Weise), and Sumitrosis terminatus (Baly) (Coleoptera: Chrysomelidae: Cassidinae) are new country records of hispine c...

  9. A coffee berry borer (Coleoptera: Curculionidae: Scolytinae) bibliography

    Science.gov (United States)

    One hundred years ago, one of the most significant biological invasions of an agricultural insect pest in the Americas was initiated. Endemic to Africa, the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae) was accidentally introduced to Brazil in 1913 and years later invaded coffe...

  10. The Peritelini (Coleoptera: Curculionidae, Entiminae of the Vitale collection

    Directory of Open Access Journals (Sweden)

    Cosimo Baviera

    2016-01-01

    Full Text Available The collection of Peritelini (Coleoptera Curculionidae Entiminae currently stored in the Vitale collection of Messina University is an element of great importance for studies of taxonomy and biogeography of these rarely collected weevils. All species are commented in relation to the contributions to this taxonomic group, published on several occasions by the authors.

  11. Predation by Flat Bark Beetles (Coleoptera: Silvanidae and Laemophloeidae) on Coffee Berry Borer (Coleoptera: Curculionidae) in Hawaii coffee

    Science.gov (United States)

    Coffee berry borer(CBB), Hypothenemus hampei, is a serious pest of coffee worldwide and a new invasive pest in Hawaii. Adult flat bark beetles, mainly Leptophloeus sp.(75%) and Cathartus quadricollis(21%) (Coleoptera: Laemophloeidae and Silvanidae, respectively), were found feeding in CBB-infested c...

  12. Het voorkomen van de glanskevers van het genus Caprophilus in Nederland (Coleoptera: Nitidulidae)

    NARCIS (Netherlands)

    Oude, de J.

    2005-01-01

    The occurrence of nitidulid beetles of the genus Carpophilus in the Netherlands (Coleoptera: Nitidulidae) Some species of the genus Carpophilus (Coleoptera: Nitidulidae) may act as pests of stored products like cocoabeans, peanuts, dried fruits like figs and dates, imported from tropical or subtropi

  13. A novel contrast stain for the rapid diagnosis of pityriasis versicolor: A comparison of Chicago Sky Blue 6B stain, potassium hydroxide mount and culture

    Directory of Open Access Journals (Sweden)

    Nikita Lodha

    2015-01-01

    Full Text Available Background: The mycological study of pityriasis versicolor is usually done by potassium hydroxide (KOH mount and culture. However, KOH mount lacks a color contrast and requires a trained eye to interpret, while culture is difficult to perform, time consuming and has low sensitivity. Chicago Sky Blue 6B (CSB is a new contrast stain that highlights the fungal hyphae and spores, blue against a purplish background. Aims and Objectives: This study was done to compare the utility of a novel contrast stain (CSB stain with KOH mount and culture. Materials and Methods: Skin scrapings from the lesions of 100 clinically diagnosed cases of P. versicolor were subjected to (1 KOH mount and CSB stain for direct microscopic examination and (2 culture using Sabouraud′s dextrose agar. The statistical analysis of CSB stain and culture was done using KOH mount as the reference method, as it is the most commonly performed and practical diagnostic test available for P. versicolor. An interrater reliability analysis using the Cohen′s Kappa statistic was performed to determine consistency (agreement among the different modalities. Observations and Results: Direct microscopy with CSB stain, KOH mount and mycological culture showed positive results in 98 (98%, 92 (92% and 56 (56% patients, respectively. Using KOH mount as the reference method, CSB stain had a sensitivity of 100% which was significantly higher than culture (60.9%. Statistically significant fair agreement was found between CSB stain and KOH mount (94% with κ=0.38, P < 0.001. Negligible agreement was found between CSB stain and culture (66%, κ=0.199, P = 0.001 as well as between KOH mount and culture (64%, κ=0.051, P = 0.107. Conclusion: CSB staining of skin scrapings is the most sensitive method for the diagnosis of pityriasis versicolor. Due to the distinct contrast provided by CSB, it is easy to perform, rapid and qualitatively superior to KOH mount.

  14. Impact of Trametes versicolor and Phanerochaete chrysosporium on differential breakdown of pesticide mixtures in soil microcosms at two water potentials and associated respiration and enzyme activity.

    OpenAIRE

    Fragoeiro, Silvia; Magan, Naresh

    2008-01-01

    This study has examined the effect of inoculation of soil microcosms with Trametes versicolor and Phanerochaete chrysosporium on wood chips on differential degradation of pesticides (simazine, trifluralin and dieldrin, 10 mg kg−1 soil) at two water potentials (−0.7 and −2.8 MPa) at 15 °C. The soil microcosms were destructively sampled after 6/12 weeks and four extracellular enzymes quantified, respiration and pesticides measured with GC and HPLC. The fungal treatments produced extracellular e...

  15. Enhanced oxidation of benzo[a]pyrene by crude enzyme extracts produced during interspecific fungal interaction of Trametes versicolor and Phanerochaete chrysosporium

    Institute of Scientific and Technical Information of China (English)

    Linbo Qian; Baoliang Chen

    2012-01-01

    The effects of interspecific fungal interactions between Trametes versicolor and Phanerochaete chrysosporium on laccase activity and enzymatic oxidation of polycyclic aromatic hydrocarbons (PAHs) were investigated.A deadlock between the two mycelia rather than replacement of one fungus by another was observed on an agar medium.The laccase activity in crude enzyme extracts from interaction zones reached a maximum after a 5-day incubation,which was significantly higher than that from regions of T.versicolor or P.chrysosporium alone.The enhanced induction of laccase activity lasted longer in half nutrition than in normal nutrition.A higher potential to oxidize benzo[a]pyrene by a crude enzyme preparation extracted from the interaction zones was demonstrated.After a 48 hr incubation period,the oxidation of benzo[a]pyrene by crude enzyme extracts from interaction zones reached 26.2%,while only 9.5% of benzo[a]pyrene was oxidized by crude extracts from T.versicolor.The oxidation was promoted by the co-oxidant 2,2'-azinobis-3-ethylbenzthiazoline-6-sulphonate diammonium salt (ABTS).These findings indicate that the application of co-culturing of white-rot fungi in bioremediation is a potential ameliorating technique for the restoration of PAH-contaminated soil.

  16. Biodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC-QqTOF-MS

    International Nuclear Information System (INIS)

    Degradation of the sulfonamide sulfamethazine (SMZ) by the white-rot fungus Trametes versicolor was assessed. Elimination was achieved to nearly undetectable levels after 20 h in liquid medium when SMZ was added at 9 mg L-1. Experiments with purified laccase and laccase-mediators resulted in almost complete removal. On the other hand, inhibition of SMZ degradation was observed when piperonilbutoxide, a cytochrome P450-inhibitor, was added to the fungal cultures. UPLC-QqTOF-MS analysis allowed the identification and confirmation of 4 different SMZ degradation intermediates produced by fungal cultures or purified laccase: desulfo-SMZ, N4-formyl-SMZ, N4-hydroxy-SMZ and desamino-SMZ; nonetheless SMZ mineralization was not demonstrated with the isotopically labeled sulfamethazine-phenyl-13C6 after 7 days. Inoculation of T. versicolor to sterilized sewage sludge in solid-phase systems showed complete elimination of SMZ and also of other sulfonamides (sulfapyridine, sulfathiazole) at real environmental concentrations, making this fungus an interesting candidate for further remediation research. - Highlights: →Degradation of sulfamethazine by Trametes versicolor was evaluated. →The laccase enzymatic system and cytochrome P-450 were involved in the degradation. →Four different degradation products of sulfamethazine were identified and confirmed. →The molecular structures and masses of the metabolites were accurately calculated. →Full elimination of sulfamethazine was observed in regular sewage sludge.

  17. Biodegradation of sulfamethazine by Trametes versicolor: Removal from sewage sludge and identification of intermediate products by UPLC-QqTOF-MS

    Energy Technology Data Exchange (ETDEWEB)

    Garcia-Galan, Ma. Jesus, E-mail: mggqam@cid.csic.es [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona (Spain); Rodriguez-Rodriguez, Carlos E., E-mail: CarlosEsteban.Rodriguez@uab.cat [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Centro de Investigacion en Contaminacion Ambiental, Universidad de Costa Rica, 2060 San Jose (Costa Rica); Vicent, Teresa, E-mail: Teresa.Vicent@uab.cat [Departament d' Enginyeria Quimica, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Caminal, Gloria, E-mail: Gloria.Caminal@uab.cat [Unitat asociada de Biocatalisi Aplicada IQAC-CSIC, Escola d' Enginyeria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona (Spain); Diaz-Cruz, M. Silvia, E-mail: sdcqam@cid.csic.es [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona (Spain); Barcelo, Damia, E-mail: dbcqam@cid.csic.es [Departament de Quimica Ambiental, IDAEA-CSIC, C/Jordi Girona 18-26, 08034 Barcelona (Spain); Catalan Institute for Water Research (ICRA), Parc Cientific i Tecnologic de la Universitat de Girona. C/Emili Grahit, 101 Edifici H2O, E-17003 Girona (Spain); King Saud University, P.O. Box 2455, Riyadh 11451 (Saudi Arabia)

    2011-11-15

    Degradation of the sulfonamide sulfamethazine (SMZ) by the white-rot fungus Trametes versicolor was assessed. Elimination was achieved to nearly undetectable levels after 20 h in liquid medium when SMZ was added at 9 mg L{sup -1}. Experiments with purified laccase and laccase-mediators resulted in almost complete removal. On the other hand, inhibition of SMZ degradation was observed when piperonilbutoxide, a cytochrome P450-inhibitor, was added to the fungal cultures. UPLC-QqTOF-MS analysis allowed the identification and confirmation of 4 different SMZ degradation intermediates produced by fungal cultures or purified laccase: desulfo-SMZ, N{sup 4}-formyl-SMZ, N{sup 4}-hydroxy-SMZ and desamino-SMZ; nonetheless SMZ mineralization was not demonstrated with the isotopically labeled sulfamethazine-phenyl-{sup 13}C{sub 6} after 7 days. Inoculation of T. versicolor to sterilized sewage sludge in solid-phase systems showed complete elimination of SMZ and also of other sulfonamides (sulfapyridine, sulfathiazole) at real environmental concentrations, making this fungus an interesting candidate for further remediation research. - Highlights: {yields}Degradation of sulfamethazine by Trametes versicolor was evaluated. {yields}The laccase enzymatic system and cytochrome P-450 were involved in the degradation. {yields}Four different degradation products of sulfamethazine were identified and confirmed. {yields}The molecular structures and masses of the metabolites were accurately calculated. {yields}Full elimination of sulfamethazine was observed in regular sewage sludge.

  18. Activation of Dormant Secondary Metabolite Production by Introducing Neomycin Resistance into the Deep-Sea Fungus, Aspergillus versicolor ZBY-3

    Directory of Open Access Journals (Sweden)

    Yuan Dong

    2014-07-01

    Full Text Available A new ultrasound-mediated approach has been developed to introduce neomycin-resistance to activate silent pathways for secondary metabolite production in a bio-inactive, deep-sea fungus, Aspergillus versicolor ZBY-3. Upon treatment of the ZBY-3 spores with a high concentration of neomycin by proper ultrasound irradiation, a total of 30 mutants were obtained by single colony isolation. The acquired resistance of the mutants to neomycin was confirmed by a resistance test. In contrast to the ZBY-3 strain, the EtOAc extracts of 22 of the 30 mutants inhibited the human cancer K562 cells, indicating that these mutants acquired a capability to produce antitumor metabolites. HPLC-photodiode array detector (PDAD-UV and HPLC-electron spray ionization (ESI-MS analyses of the EtOAc extracts of seven bioactive mutants and the ZBY-3 strain indicated that diverse secondary metabolites have been newly produced in the mutant extracts in contrast to the ZBY-3 extract. The followed isolation and characterization demonstrated that six metabolites, cyclo(d-Pro-d-Phe (1, cyclo(d-Tyr-d-Pro (2, phenethyl 5-oxo-l-prolinate (3, cyclo(l-Ile-l-Pro (4, cyclo(l-Leu-l-Pro (5 and 3β,5α,9α-trihydroxy-(22E,24R-ergosta-7,22-dien-6-one (6, were newly produced by the mutant u2n2h3-3 compared to the parent ZBY-3 strain. Compound 3 was a new compound; 2 was isolated from a natural source for the first time, and all of these compounds were also not yet found in the metabolites of other A. versicolor strains. Compounds 1–6 inhibited the K562 cells, with inhibition rates of 54.6% (1, 72.9% (2, 23.5% (3, 29.6% (4, 30.9% (5 and 51.1% (6 at 100 μg/mL, and inhibited also other human cancer HL-60, BGC-823 and HeLa cells, to some extent. The present study demonstrated the effectiveness of the ultrasound-mediated approach to activate silent metabolite production in fungi by introducing acquired resistance to aminoglycosides and its potential for discovering new compounds from silent

  19. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, Jessica; Verheggen, François; Lognay, Georges; Haubruge, Eric

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and...

  20. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, J.; Verheggen, F.; Lognay, G.; Haubruge, E.

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and ...

  1. Pomegranate peels: A novel ago-residue for tannate production by gamma irradiated aspergillus versicolor under solid state fermentation

    International Nuclear Information System (INIS)

    Pomegranate peels were tested as a substrate for the production of tannate and gallic acid (GA) under solid state fermentation (SSF) using Aspergillus versicolor-g3B (highly potent gamma irradiated tannate producer isolate). The fungal strain was grown on the substrate without any pre-treatment. Different parameters for maximum production of tannate by this strain under SSF method were optimized. The maximum tannate yield (12.45μg-1ds) and GA (94%) were obtained when SSF was carried out at ph 5.0, 30 degree C, 60% initial substrate moisture, 20% inoculum size, 3% tannic acid and 1% ammonium nitrate as additional carbon and nitrogen sources, after 96 h of fermentation. The crude tannate from this isolate showed ph and temperature optima of 5.0 and 50 degree C, respectively. Also, the enzyme was stable in a broad ph range of 3-8 for 24 h and was also heat stable as it retained 65% activity at 70 degree C after 1 hour

  2. DECOLORIZATION OF TEXTILE DYES BY NEWLY ISOLATED TRAMETES VERSICOLOR STRAIN Sevil PİLATİN, Buket KUNDUHOĞLU

    Directory of Open Access Journals (Sweden)

    Buket KUNDUHOĞLU

    2011-08-01

    Full Text Available In this century, the amount of industrial produces and their consumption has increased tremendously. Along with this increase, accumulation of industrial waste and its effect on nature has causedserious problems. Because of including various chemicals and especially dye, textile waste water is one of the most hazardous industrial wastes. Color is the most important pollutant in waste water and it should be decolorized. Decolorization is more important than degradation of organic substances from waste water. Even a small amount of dye found changes the color of rivers, lakes and other waterresources, and reduces the penetration of light and solubility of gases. White rot fungus are used as a biological system in degradation and decolorizing of textile dyes.In this study, parameters for decolorization of several textile dyes (Blue 49, Orange 12, Orange 13, Red 31, Black 5, RBBR by newly isolated Trametes versicolor M96was studied.It has been determined that pH, amount of inoculum, shaking speed (rpm, dye concentration and temperature are important factors in decolorization of the studied dyes. The maximum decolorization was found pH 4.5, amount of inoculums 50 ml, shaking speed 200 rpm, dye concentration 50 mg/l and heat 30 °C.

  3. Desulphurization of some low-rank Turkish lignites with crude laccase produced from Trametes versicolor ATCC 200801

    Energy Technology Data Exchange (ETDEWEB)

    Aytar, Pinar; Gedikli, Serap [Graduate School of Natural and Applied Sciences, Eskisehir Osmangazi University (Turkey); Sam, Mesut [Department of Biology, Faculty of Arts and Science, Aksaray University (Turkey); Uenal, Arzu [Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Research, Ankara (Turkey); Cabuk, Ahmet [Department of Biology, Faculty of Arts and Science, Eskisehir Osmangazi University (Turkey); Kolankaya, Nazif [Department of Biology, Division of Biotechnology, Faculty of Science, Hacettepe University, Ankara (Turkey); Yueruem, Alp [Grand Water Research Institute, Technion Israel Institute of Technology, Haifa (Israel)

    2011-01-15

    In this paper, data obtained during the oxidative desulphurization of some low-rank Turkish lignites with crude laccase enzyme produced from Trametes versicolor ATCC 200801 are presented. In order to optimize desulphurization conditions, effects of incubation time, pulp density, incubation temperature, medium pH, and also lignite source on the desulphurization have been examined. The values for incubation period, pulp density, temperature and pH in optimum incubation condition were found as 30 min, 5%, 35 C, and pH 5.0, respectively. Under optimum conditions, treatment of coal samples with crude laccase has caused nearly 29% reduction in their total sulphur content. During the study, the rate of desulphurization of coal sample provided from Tuncbilek with crude laccase was found to be relatively higher than the other examined coal samples. Results of analytical assays have indicated that the treatment of coals with crude laccase has caused no change in their calorific values but reduced their sulphur emissions. 35%, 13%, and 25% reductions of pyritic sulphur, sulphate and organic sulphur in a period of 30 min were achieved, for a particle size of 200 {mu}m under optimal conditions with enzymatic desulphurization. Also, statistical analyses such as Tukey Multiple Comparison tests and ANOVA were performed. (author)

  4. Extracellular polysaccharide with novel structure and antioxidant property produced by the deep-sea fungus Aspergillus versicolor N2bc.

    Science.gov (United States)

    Yan, Meng-Xia; Mao, Wen-Jun; Liu, Xue; Wang, Shu-Yao; Xia, Zheng; Cao, Su-Jian; Li, Jing; Qin, Ling; Xian, Hua-Li

    2016-08-20

    An extracellular polysaccharide, N1, was obtained from the culture medium of the deep-sea fungus Aspergillus versicolor N2bc by a combination of ethanol precipitation, ion-exchange and gel filtration chromatography. N1 was a mannoglucogalactan with molecular weight of about 20.5kDa. Results of chemical and spectroscopic analyses, including Fourier-transform infrared, one- and two-dimensional nuclear magnetic resonance spectroscopy showed that the main chain of N1 consisted of →2)-α-d-Glcp-(1→, →2)-β-d-Glcp-(1→ and →6)-β-d-Manp-(1→ units, substituted at C-6 position of →2)-α-d-Glcp-(1→ units. The branches were composed of galactofuranose-oligosaccharides built up of →5)-β-d-Galf-(1→, →6)-β-d-Galf-(1→ and terminal β-d-Galf units. At an average, there were two branching points for every five sugar residues in the backbone. N1 possessed a high in vitro antioxidant activity as evaluated by scavenging assays involving superoxide, 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radicals and reducing power. The investigation revealed that N1 was a novel antioxidant polysaccharide differing from previously described extracellular polysaccharides and could be a potential antioxidant. PMID:27178933

  5. Polyamide 6/chitosan nanofibers as support for the immobilization of Trametes versicolor laccase for the elimination of endocrine disrupting chemicals.

    Science.gov (United States)

    Maryšková, Milena; Ardao, Inés; García-González, Carlos A; Martinová, Lenka; Rotková, Jana; Ševců, Alena

    2016-07-01

    In recent years, there has been an increase in efforts to improve wastewater treatment as the concentration of dangerous pollutants, such as endocrine disrupting chemicals, in wastewater increases. These compounds, which mimic the effect of hormones, have a negative impact on human health and are not easily removed from water. One way to effectively eliminate these pollutants is to use enzymatically activated materials. In this study, we report on the use of laccase from the white rot fungus Trametes versicolor immobilized onto polyamide 6/chitosan (PA6/CHIT) nanofibers modified using two different spacers (bovine serum albumin and hexamethylenediamine). We then tested the ability of the PA6/CHIT-laccase biocatalysts to eliminate a mixture containing 50μM of two endocrine disrupting chemicals: bisphenol A and 17α-ethinylestradiol. The PA6/CHIT nanofiber matrix used in this study not only proved to be a suitable carrier for immobilized and modified laccase but was also efficient in the removal of a mixture of endocrine disrupting chemicals in three treatment cycles. PMID:27233125

  6. Liquefaction of coal by Polyporus versicolor and Poria monticola. Final report, 1 September 1984-31 August 1986

    Energy Technology Data Exchange (ETDEWEB)

    Cohen, M.S.

    1986-01-01

    Polyporus versicolor (ATCC 12679), obtained from the American Type Culture Collection, Rockville, MD, has been demonstrated to degrade leonardite, lignite, and subbituminous coals to a black liquid product which is called the bioextract. The process of solubilizing the coal has been termed liquification. Fungi were routinely maintained in both solid Sabouraud maltose agar (6%) and in Sabouraud maltose broth cultures. All cultures were incubated at 30/sup 0/C, 84 to 98% relative humidity, and pH = 5.8. All materials which came into contact with the fungi were sterilized before use. Experimental cultures were incubated as described for stock cultures. Cultures were incubated for approximately 12 days to produce a mature fungal mat across a glass petri dish. Coal pieces (approximately 5 mm/sup 3/) were placed directly on the hyphal mat. Liquified coal (the bioextract) was removed from the top of the mycelium and/or coal pieces and either stored for analyses at 4/sup 0/C or else freeze-dried and stored dessicated at room temperature. The bioextract has been produced in sufficient quantity to permit various methods of analysis including high performance liquid chromatography, UV-visible spectrophotometry, titrimetry, electrophoresis, proton nmr spectroscopy, and calorimetry. The solubility of the bioextract in different solvents has also been determined. 6 refs., 26 figs., 3 tabs.

  7. Nocturnal Migration of Coleoptera: Carabidae in North China

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Vertical-looking radar (VLR) has allowed long-term automatic monitoring of the altitudinal and temporal dynamics of high-flying insect populations. To investigate whether ground beetle, insect of Coleoptera, was capable of migrating and its migration pattern by taking advantages of capability of the VLR for long-term real-time automatic monitoring, the migration of Coleoptera ground beetle was investigated by setting up radar observation points, making long-term observation using the VLR and related supplementary equipment, and analyzing low altitude air current and large area circulating current in combination with the meteorological data. Information obtained in 2005 and 2006 showed that the seasonal activities of ground beetles traps of trap lamps were mainly from late June to late August, peak period was mainly in August, seasonal traps of high-altitude lamps and ground lamps were featured by sudden increase and sudden decrease; in peak period, the height of radar echo point could be as high as 600 m, while it was mainly below the height of 450 m; night activities mainly occurred from 20:00 to 22:00, in very few nights, radar echo could last until about 04:00, changes in numbers of ground beetles within the searchlights were consistent with radar echo intensity; ground beetle images were successfully trapped in the sweep nets carried by captive balloons at the height of 200 m. Some species of Carabidaes had some degrees of migration, thus providing the foundation for investigating the migration of Coleoptera insects.

  8. Estados inmaduros de Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini Immature stages of Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini

    Directory of Open Access Journals (Sweden)

    Jhon César Neita-Moreno

    2008-12-01

    Full Text Available Se describen y se ilustran por primera vez la larva de tercer estadio y la pupa de Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini. Se aportan datos sobre la biología de la especie y su distribución en Colombia. Se proporciona una clave para la identificación de las larvas de tercer estadio conocidas de las especies del género Ancognatha Erichson.The third instar larva and pupa of Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini are described and illustrated for the first time. New data on larval biology and distribution of the species in Colombia are included. A key to the known third stage larvae of Ancognatha Erichson is provided.

  9. Family-Group Names In Coleoptera (Insecta

    Directory of Open Access Journals (Sweden)

    Patrice Bouchard

    2011-04-01

    Full Text Available We synthesize data on all known extant and fossil Coleoptera family-group names for the first time. A catalogue of 4887 family-group names (124 fossil, 4763 extant based on 4707 distinct genera in Coleoptera is given. A total of 4492 names are available, 183 of which are permanently invalid because they are based on a preoccupied or a suppressed type genus. Names are listed in a classification framework. We recognize as valid 24 superfamilies, 211 families, 541 subfamilies, 1663 tribes and 740 subtribes. For each name, the original spelling, author, year of publication, page number, correct stem and type genus are included. The original spelling and availability of each name were checked from primary literature. A list of necessary changes due to Priority and Homonymy problems, and actions taken, is given. Current usage of names was conserved, whenever possible, to promote stability of the classification. New synonymies (family-group names followed by genus-group names: Agronomina Gistel, 1848 syn. n. of Amarina Zimmermann, 1832 (Carabidae, Hylepnigalioini Gistel, 1856 syn. n. of Melandryini Leach, 1815 (Melandryidae, Polycystophoridae Gistel, 1856 syn. n. of Malachiinae Fleming, 1821 (Melyridae, Sclerasteinae Gistel, 1856 syn. n. of Ptilininae Shuckard, 1839 (Ptinidae, Phloeonomini Ádám, 2001 syn. n. of Omaliini MacLeay, 1825 (Staphylinidae, Sepedophilini Ádám, 2001 syn. n. of Tachyporini MacLeay, 1825 (Staphylinidae, Phibalini Gistel, 1856 syn. n. of Cteniopodini Solier, 1835 (Tenebrionidae; Agronoma Gistel 1848 (type species Carabus familiaris Duftschmid, 1812, designated herein syn. n. of Amara Bonelli, 1810 (Carabidae, Hylepnigalio Gistel, 1856 (type species Chrysomela caraboides Linnaeus, 1760, by monotypy syn. n. of Melandrya Fabricius, 1801 (Melandryidae, Polycystophorus Gistel, 1856 (type species Cantharis aeneus Linnaeus, 1758, designated herein syn. n. of Malachius Fabricius, 1775 (Melyridae, Sclerastes Gistel, 1856 (type species

  10. Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

    Science.gov (United States)

    Libert, X; Chasseur, C; Bladt, S; Packeu, A; Bureau, F; Roosens, N H; De Keersmaecker, S C J

    2015-09-01

    Currently, contamination of indoor environment by fungi and molds is considered as a public health problem. The monitoring of indoor airborne fungal contamination is a common tool to help understanding the link between fungi in houses and respiratory problems. Classical analytical monitoring methods, based on cultivation and microscopic identification, depend on the growth of the fungi. Consequently, they are biased by difficulties to grow some species on certain culture media and under certain conditions or by noncultivable or dead fungi that can consequently not be identified. However, they could have an impact on human health as they might be allergenic. Since molecular methods do not require a culture step, they seem an excellent alternative for the monitoring of indoor fungal contaminations. As a case study, we developed a SYBR® green real-time PCR-based assay for the specific detection and identification of Aspergillus versicolor, which is frequently observed in indoor environment and known to be allergenic. The developed primers amplify a short region of the internal transcribed spacer 1 from the 18S ribosomal DNA complex. Subsequently, the performance of this quantitative polymerase chain reaction (qPCR) method was assessed using specific criteria, including an evaluation of the selectivity, PCR efficiency, dynamic range, and repeatability. The limit of detection was determined to be 1 or 2 copies of genomic DNA of A. versicolor. In order to demonstrate that this SYBR® green qPCR assay is a valuable alternative for monitoring indoor fungal contamination with A. versicolor, environmental samples collected in contaminated houses were analyzed and the results were compared to the ones obtained with the traditional methods. PMID:26184975

  11. Produção, isolamento e caracterização química do exopolissacarídeo do fungo da podridão branca da madeira Tramentes versicolor

    OpenAIRE

    Pereira, Susana Raquel de Sousa

    2009-01-01

    O Trametes versicolor é um fungo da podridão branca da madeira com potencial aplicação como oxidante na indústria papeleira. Segundo a literatura, este fungo produz também uma (1-3)--glucana com actividade imunomoduladora, propriedades anti-tumorais e anti-microbianas. O objectivo deste trabalho foi produzir, isolar, solubilizar e caracterizar quimicamente o exopolissacarídeo (EPS) do fungo T. versicolor. O fungo foi produzido por fermentação em cultura líquida, tendo o material polimérico s...

  12. Primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae and Disteniidae) of the Smithsonian Institution

    Science.gov (United States)

    The primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae, Disteniidae) of the National Museum of Natural History (Smithsonian Institution) are catalogued and figured, current through 2012 (but also including some 2013 holotypes). Data on the original combination, current combina...

  13. New records of predaceous diving beetles (Coleoptera:Dytiscidae) in Maine

    Science.gov (United States)

    Boobar, L.R.; Gibbs, K.E.; Longcore, J.R.; Perillo, A.M.

    1996-01-01

    Locations, habitat descriptions, and collection dates are listed for new records of 4 genera and 12 species of predaceous diving beetles (Coleoptera: Dytiscidae) in Maine. Previously, 17 genera and 53 species of the aquatic beetle were reported from Maine.

  14. First record of Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae in Brazil

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2015-03-01

    Full Text Available Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae is recorded for the first time in Brazil (Bahia. It was originally described from Europe and is currently widely distributed in that continent and Asia.

  15. Methods for assessing infestations of sunflower stem weevil (Coleoptera: Curculionidae) in sunflower stems

    Science.gov (United States)

    The sunflower stem weevil, Cylindrocopturus adspersus LeConte (Coleoptera: Curculionidae), reduces sunflower, Helianthus annuus L. (Asteraceae), yields by spreading pathogens, damaging vascular tissues, and promoting lodging of sunflower plants. To assess weevil populations for host plant resistanc...

  16. A new species of the genus Falsoibidion Pic (Coleoptera, Cerambycidae) from Korea

    OpenAIRE

    Lee, Seunghyun; Lee, Seunghwan

    2016-01-01

    A new species of the genus Falsoibidion Pic, 1922 (Coleoptera, Cerambycidae, Cerambycinae, Callidiopini) from Korea is described. Habitus and genitalia of male and female of the new species are illustrated.

  17. Biologi Hama Kumbang Penggerek Pucuk Kelapa Sawit (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) Di Rumah Kassa

    OpenAIRE

    Sejahtra, Ahmad

    2011-01-01

    Ahmad Sejahtra, "BIOLOGY SHOOT BORERS BEETLE PEST OF COCONUT PALM (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) AT KASSA HOUSE", Under supervised by Ms Marheni and Ms Fatima Zahara. This study aims to determine biological pest of oil palm bud weevil (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) at home kassa. The experiment was conducted on people's plantations in PERUMNAS Simalingkar starting from September 2010 until April 2011. Research using observational methods for this resear...

  18. Papel dos besouros (Insecta, Coleoptera) na Entomologia Forense

    OpenAIRE

    Wellington Emanuel dos Santos

    2014-01-01

    Esse trabalho apresenta uma revisão do papel que os besouros (Insecta, Coleoptera) desempenham na Entomologia Forense. Discussões sobre ocorrência em cadáveres humanos e carcaças animais, estimativas de Intervalo Pós-Morte (IPM), estudos realizados no Brasil e em outros países, principais famílias de importância forense e aspectos biológicos, ecológicos e biogeográficos das espécies são apresentadas.

  19. Fossil history of Mesozoic weevils (Coleoptera:Curculionoidea)

    Institute of Scientific and Technical Information of China (English)

    Andrei A.Legalov

    2012-01-01

    The first synopsis of Mesozoic weevils (Curculionoidea: Coleoptera) is presented.Changes of family,genera and species abundance during the Mesozoic revealed three distributional patterns.The Jurassic (Karatau) fauna was dominated by the Nemonychidae.During the Early Cretaceous (beginning at the Jurassic/Cretaceous border),the Ithyceridae was the prevalent group with a significant role played by the Nemonychidae.In the Late Cretaceous (Cenomanian and Turonian),the major groups were the Curculionidae and Brentidae.Obviously,the change of weevil fauna during this period was due to the expansion of the angiosperms,which provided multiple niches in their vegetative and reproductive organs for weevil development.

  20. An annotated catalogue of the Buprestidae of Iran (Coleoptera: Buprestoidea).

    Science.gov (United States)

    Ghahari, Hassan; Volkovitsh, Mark G; Bellamy, Charles L

    2015-01-01

    An annotated taxonomic catalogue of the jewel beetles (Coleoptera: Buprestidae) of Iran is given. Original descriptions and recent revisionary or catalogue data are included along with the distribution, both within and outside of Iran, ecological data and host plant associations, junior synonyms, and comments. A complete bibliography completes the catalogue. In total 428 species and 52 subspecies of jewel beetles belonging to 6 subfamilies (Julodinae, Polycestinae, Galbellinae, Chrysochroinae, Buprestinae, and Agrilinae), 20 tribes, and 38 genera are known from Iran including doubtful records and 4 nomina nuda. It is likely that the number of jewel beetle species from Iran will be between 460-480 and possibly even more species. PMID:26250020

  1. Batch production of Pyranose 2-oxidase from Trametes versicolor (ATCC 11235) in medium with a lignocellulosic substrate and enzymatic bleaching of cotton fabrics.

    Science.gov (United States)

    Pazarlioglu, Nurdan Kasikara; Erden, Emre; Ucar, M Cigdem; Akkaya, Alper; Sariisik, A Merih

    2012-04-01

    The aim of this work was to determine new, different and low-cost substrates that can be used for enzyme production from the white rot fungus Trametes versicolor (ATCC 11235) by taking advantage of the broad substrate specificity of pyranose 2-oxidase. In this report, we investigated the production of pyranose 2-oxidase from T. versicolor (ATCC 11235) using ten different agricultural residues such as clover straw, almond shells, hazelnut cobs, grass and others. Pyranose 2-oxidase activity was determined as 2.332 U/g at the 9th day in a submerged culture containing clover straw and tap water shaken at 150 rpm and 26°C, and the optimum clover straw concentration was determined to be 12 g/l. The effects of different glucose, nitrogen and phosphate sources on the production of pyranose 2-oxidase were studied in the clover straw medium. Analyses of biomass, protein, reduced sugar and nitrogen concentrations were also monitored in a clover straw medium that did not contain carbon or nitrogen and phosphate sources under the parameters determined. The produced pyranose 2-oxidase was used for improving the properties of cotton fabrics. PMID:22805934

  2. A comparative study on decolorization of reactive azo and indigoid dyes by free/immobilized pellets of Trametes versicolor and Funalia trogii.

    Science.gov (United States)

    Yildirim, Seval Cing; Yesilada, Ozfer

    2015-11-01

    The objective of the present study was to investigate decolorization of Acid Blue 74 and Reactive Blue 198 dyes by free and immobilized white rot fungal pellets in order to confirm the possibility of practical application via repeated-batch cultivation. Decolorization studies were conducted using free pellets (FP), fungal cells immobilized on activated carbon (IFCAC) and pinewood (IFCP), and also fungal cells entrapped in alginate beads (FCEAB). No additional nitrogen and carbon source was used and high decolorization rates were achieved in only dye-contained media without pH adjustment. Acid Blue 74 was decolorized 96 and 94% within 2 hr by Trametes versicolor and Funalia trogii free pellets, respectively. These values were 87 and 84% for Reactive Blue 198, in this respect. Immobilization of fungal cells on pinewood increased the usability of pellets and the average decolorization efficiency of both dyes. The micro environment changed in the presence of pinewood and increased the stability of immobilized pellets. Decolorization was performed rapidly and efficiently. Laccase activity enhanced with availability of pinewood, and high laccase production with F. trogii was obtained. After separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the molecular weight of T versicolor and F. trogii laccase bands was determined 64 and 61 kDa approximately. Green bands were obtained by the activity staining process with laccase substrate (ABTS) after gel renaturation step. PMID:26688979

  3. Polysaccharide peptides from Coriolus versicolor exert differential immunomodulatory effects on blood lymphocytes and breast cancer cell line MCF-7 in vitro.

    Science.gov (United States)

    Kowalczewska, Małgorzata; Piotrowski, Jakub; Jędrzejewski, Tomasz; Kozak, Wiesław

    2016-06-01

    The protein-bound polysaccharides (PBP), isolated from Coriolus versicolor (CV) fungus, are considered as natural compounds with potential therapeutic applications. The immunopotentiating and antitumor activity of polysaccharopeptides has been previously examined, however similar findings could not be achieved. The source of PBP, variations in extraction process as well as environmental factors seems to affect the biological properties of these active CV components. Since further analysis are needed to draw more definite conclusion, the present study aimed to investigate the immunomodulatory properties of the PBP extract, isolated from commercially available capsules of C. versicolor. Our results revealed that the effect mediated by PBP extract depends on the target cells. We reported that the polysaccharopeptides induced a significant decrease in breast cancer MCF-7 cells growth, which was TNF-α-dependent phenomenon. Interestingly, the level of two others cytokines, IL-1β and IL-6 was not affected. On the other hand, in this study we noticed that protein-bound polysaccharides extracted from CV significantly augmented the proliferative response of blood lymphocytes in a time-dependent manner, which was associated with IL-6 and IL-1β mRNA upregulation. Moreover we found that the cells response to PBP stimuli might be inversely related to its concentration. PMID:27091479

  4. Characterization of purified and Xerogel immobilized Novel Lignin Peroxidase produced from Trametes versicolor IBL-04 using solid state medium of Corncobs

    Directory of Open Access Journals (Sweden)

    Asgher Muhammad

    2012-08-01

    Full Text Available Abstract Background Cost-effective production of industrially important enzymes is a key for their successful exploitation on industrial scale. Keeping in view the extensive industrial applications of lignin peroxidase (LiP, this study was performed to purify and characterize the LiP from an indigenous strain of Trametes versicolor IBL-04. Xerogel matrix enzyme immobilization technique was applied to improve the kinetic and thermo-stability characteristics of LiP to fulfil the requirements of the modern enzyme consumer sector of biotechnology. Results A novel LiP was isolated from an indigenous T. versicolor IBL-04 strain. T. versicolor IBL-04 was cultured in solid state fermentation (SSF medium of corn cobs and maximum LiP activity of 592 ± 6 U/mL was recorded after five days of incubation under optimum culture conditions. The crude LiP was 3.3-fold purified with specific activity of 553 U/mg after passing through the DEAE-cellulose and Sephadex-G-100 chromatography columns. The purified LiP exhibited a relatively low molecular weight (30 kDa homogenous single band on native and SDS-PAGE. The LiP was immobilized by entrapping in xerogel matrix of trimethoxysilane (TMOS and proplytetramethoxysilane (PTMS and maximum immobilization efficiency of 88.6% was achieved. The free and immobilized LiPs were characterized and the results showed that the free and immobilized LiPs had optimum pH 6 and 5 while optimum temperatures were 60°C and 80°C, respectively. Immobilization was found to enhance the activity and thermo-stability potential of LiP significantly and immobilized LiP remained stable over broad pH and temperature range as compare to free enzyme. Kinetic constants Km and Vmax were 70 and 56 μM and 588 and 417 U/mg for the free and immobilized LiPs, respectively. Activity of this novel extra thermo-stable LiP was stimulated to variable extents by Cu2+, Mn2+ and Fe2+ whereas, Cystein, EDTA and Ag+ showed inhibitory effects

  5. Effect of Coriolus Versicolor Polysaccharide-B on the Biological Characteristics of Human Esophageal Carcinoma Cell Line Eca109

    International Nuclear Information System (INIS)

    To investigate the effect of Coriolus versicolor polysaccharide-B (CVPs-B) on the biological characteristics of human esophageal carcinoma cell line Eca109 in vitro. The cells of experimental group (EG) were cultured in DMEM with 10% FCS and 150µg/mL CVPs-B, the cells of control group (CG) were cultured in DMEM with 10% FCS without CVPs-B. MTT reduction assay was performed to detect the effect of CVPs-B on the proliferation of Eca109 cells after the compound was administrated in varying concentrations. The living conditions of the Eca109 cells were determined using trypan blue exclusion. Then, cell growth curves were drawn. Flow cytometry was performed to detect the effect of CVPs-B on the apoptosis and cell cycle of Eca109. In comparison with the CG, a marked decrease in the proliferation of Eca09 cells was observed in the EG, after incubation with CVPs-B. The survival rate of Eca09 cells decreased as the time of CVPs-B incubation prolonged. Comparing the cell cycles and apoptotic rates between the two groups, the proportions of cells in the G0/G1, S, and G2/M phases in the EG were found to be (68.4±3.7)%, (13.9±2.1)%, and (17.7±1.4)%, respectively, after 24 h incubation with CVPs-B. The cells had an apoptotic rate of (9.7±0.7)%. On the other hand, the proportions of the G0/G1, S, and G2/M cells of the CG were found to be (53.9±3.6)%, (26.6±2.8)%, and (19.5±2.3)%, respectively, with an apoptotic rate of (5.7±1.4)%. In comparison with the CG cells, significant cell growth in the G0/G1 phase was observed in the EG (P<0.05). Furthermore, a significant decrease in the number of cells in the S phase was observed (P<0.05) in the EG. CVPs-B can inhibit proliferation and enhance apoptosis of Eca109 cells and may be useful in the treatment of esophageal carcinoma

  6. The complete mitochondrial genome of Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae).

    Science.gov (United States)

    Sun, Tian Yi; Li, Lei; Xin, Tianrong; Wang, Yayu; Xia, Bin

    2016-09-01

    We determined the complete mitochondrial genome (mitogenome) sequence of Cryptolestes ferrugineus (GenBank accession number KT182067) by the long PCR and primer walking method. The mitochondrial genome is a typical circular DNA molecule of 15 511 bp in length, and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region (D-loop). The order of 37 genes was typical of insect mitochondrial DNA sequences described to date. The base composition of the genome is A (39.17%), T (37.24%), C (14.22%), and G (9.37%) with an A + T-rich hallmark as that of other invertebrate mitochondrial genomes. All protein-coding genes start with ATN codon and terminate with the stop codon T (AA) or TAG. The A + T-rich region is located between 12S rRNA and tRNA(Ile). In this study, the phylogenetic relationships of Coleoptera species were constructed based on the nucleotides sequences of 13 PCGs of mitogenomes. The molecular-based phylogeny supported the traditional morphological classification on relationships within Coleoptera species. PMID:26330111

  7. Rove beetles of medical importance in Brazil (Coleoptera, Staphylinidae, Paederinae

    Directory of Open Access Journals (Sweden)

    Juliana S. Vieira

    2014-09-01

    Full Text Available Rove beetles of medical importance in Brazil (Coleoptera, Staphylinidae, Paederinae. The rove beetles of the genus Paederus Fabricius, 1775 are the most important group within Coleoptera causing dermatitis around the world. The medical importance of Paederus depends on its toxic hemolymph released when these beetles are crushed on human skin. The effects are mainly dermatitis linearis and some sporadic cases of conjunctivitis. In Brazil seven species of Paederus are known to cause dermatitis: P. amazonicus Sharp, 1876, P. brasiliensis Erichson, 1840, P. columbinus Laporte, 1835, P. ferus Erichson, 1840, P. mutans Sharp, 1876, P. protensus Sharp, 1876 stat. rev., and Paederus rutilicornis Erichson, 1840. Paederus mutans and P. protensus are for the first time recorded as of medical importance, whereas the record of P. rutilicornis in Brazil is doubtful. All seven species are redescribed and a dichotomous key is provided. The geographic distributions of all species are documented. The results provided here include the most recent and relevant taxonomic revision of Paederus of the Neotropical region, the first identification key for Brazilian species and the increase of recorded species of medical importance in the world.

  8. Complete mitochondrial genome of Cryptolestes pusillus (Coleoptera: Laemophloeidae).

    Science.gov (United States)

    Li, Lei; Liu, Guanghua; Sun, Tanyi; Xin, Tianrong; Li, Meiyun; Zou, Zhiwen; Xia, Bin

    2016-09-01

    In this study, the complete mitochondrial genome of Cryptolestes pusillus (GenBank accession number KT070713) was sequenced by long PCR and primer walking methods. The total length of mitochondrial DNA is 15 502 bp and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region. The base composition of the genome is A (39.04%), T (37.07%), C (23.4%), and G (14.6%). Except for COI and ATP8 with TCC and ATC as start codon, respectively, the remaining protein-coding genes initiated with the three orthodox start codons. Two complete stop codons (TAA and TAG) and two incomplete stop codons (COIII stop with T and ND5 stop with TA) were used in the protein-coding genes. The A + T-rich region is located between 12s rRNA and tRNA(Ile) with the length of 859 bp. The phylogenetic relationships of Coleoptera species were constructed based on the nucleotide sequences of 13 protein-coding genes of mitogenome using the neighbor-joining method. The molecular-based phylogenetic analysis supported the traditional morphological classification on relationships within Coleoptera species. PMID:26329895

  9. 载体吸附培养黄孢原毛平革菌(Phanerochaete chrysosporium)和云芝(Trametes versicolor) 对染料的脱色%Decolorization of dyes by Phanerochaete chrysosporium and Trametes versicolor growing on adsorptive carriers

    Institute of Scientific and Technical Information of China (English)

    张昊; 李学梅; 张文黎; 花清信; 苌道松; 徐颖; 李雪玲; 任四伟; 陈宣宇; 杨清香

    2012-01-01

    Phanerochaete chrysosporium and Trametes versicolor were cultured on different carriers for continuous decolorization, the performance of different Phanerochaete chrysosporium and Trametes versicolor were researched and compared. In this paper, Phanerochaete chrysosporium and Trametes versicolor were cultured in liquid containing spherical sawdust, corncob and peanut shells under oscillation conditions. During few days of cultivation, large amount of bacteria was grew and attached on the carrier surface in membranous and lump state. After consecutive 4 rounds of decolorization, Phanerochaete chrysosporium and Trametes versicolor growing on sawdust carrier had significant advantage both in continuous decolorization and producing enzymes, so the optimal adsorptive carrier was sawdust. After consecutive 2 rounds and 12 d of reactive black RB5 decolorization, the Phanerochaete chrysosporium culture with sawdust as adsorptive carrier could achieve the decolorization of 97 %; after three rounds of dye supplemented, the cultures also could remove nearly 96% of M-3BE and the maximum production of manganese dependant pcroxidase enzyme (MnP) and lignin peroxidase enzyme (LiP) was 611, 1 477 U/L. In the actual application, Phanerochaete chrysosporium and Trametes versicolor culture with sawdust as carriers should be added to dye wastewater treatment system to strengthen the biological treatment effect.%比较研究了不同载体吸附培养的黄孢原毛平革菌(Phanerochaete chrysosporium)和云芝(Trametes versicolor)对染料连续脱色的效果.结果表明:(1)白腐真菌黄孢原毛平革菌和云芝分别在含有球形的木屑、玉米芯和花生壳载体的液体环境中振荡培养,菌体以膜状或团状形式大量附着生长在载体表面.(2)连续4轮脱色过程中,不论黄孢原毛平革菌还是云芝,都是木屑为载体的培养液的持续脱色和产酶能力最好,宜选择木屑为载体.其中,木屑为载体的黄孢

  10. Chaves para a identificação dos principais Coleoptera (Insecta) associados com produtos armazenados Keys for the identification of Coleoptera (Insecta) associated with stored products

    OpenAIRE

    Paulo Roberto Valle da Silva Pereira; Lúcia Massutti de Almeida

    2001-01-01

    An illustrated key to identify nine families of Coleoptera commonly found in stored products is presented. Keys for the identification of Anobiidae [Lasioderma serricorne (Fabricius, 1792), Stegobium paniceum (Linnaeus, 1761)], Bruchidae [Acanthoscelides obtectus (Say, 1831), Zabrotes subfasciatus (Boheman, 1833)], Curculionidae [Sitophilus oryzae (Linnaeus, 1763), S. zeamais Motschulsky, 1885], Silvanidae [Ahasverus advena (Waltl, 1832), Cathartus quadricollis (Guérin, 1892), Oryzaephilus me...

  11. Optimization of C/N Ratio and Inducers for Wastewater Paper Industry Treatment Using Trametes versicolor Immobilized in Bubble Column Reactor

    Directory of Open Access Journals (Sweden)

    Aura M. Pedroza-Rodríguez

    2013-01-01

    Full Text Available C/N ratio and MnSO4 and CuSO4 concentrations were optimized for decolorization and chemical oxygen demand (COD removal of bleached Kraft pulp mill effluent by Trametes versicolor immobilized in polyurethane foam. Statistical differences (P<0.0001 at high C/N ratios (169, 2 mM CuSO4, and 0.071 mM MnSO4 were determined. Decolorization of 60.5%, COD removal of 55%, laccase (LAC 60 U/L, and manganese peroxidase (MnP 8.4 U/L were obtained. Maximum of decolorization (82%, COD removal (83%, LAC (443.5 U/L, and MnP (18 U/L activities at C/N ratio of 405 (6.75 mM CuSO4 and 0.22 mM MnSO4 was achieved in step 7 at 4 d. Positive correlation between the decolorization, COD removal, and enzymatic activity was found (P<0.0001. T. versicolor bioremediation capacity was evaluated in bubble column reactor during 8 d. Effluent was adjusted according to optimized parameters and treated at 25°C and air flow of 800 mL/min. Heterotrophic bacteria growth was not inhibited by fungus. After 4 d, 82% of COD reduction and 80% decolorization were recorded. Additionally, enzymatic activity of LAC (345 U/L and MnP (78 U/L was observed. The COD reduction and decolorization correlated positively (P<0.0001 with enzymatic activity. Chlorophenol removal was 98% of pentachlorophenol (PCP, 92% of 2,4,5-trichlorophenol (2,4,5-TCP, 90% of 3,4-dichlorophenol (3,4-DCP, and 99% of 4-chlorophenols (4CP.

  12. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Sri Puji Astuti Wahyuningsih

    2016-03-01

    Full Text Available Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1 and adding polysaccharide krestin before-after exposure (P3 had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2 had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1, 12-17.

  13. Biology and Management of Billbugs (Coleoptera: Curculionidae) in Turfgrass

    Science.gov (United States)

    Dupuy, Madeleine M.; Ramirez, Ricardo A.

    2016-01-01

    Billbugs (Coleoptera: Curculionidae: Sphenophorus spp.) are a complex of weevil pests affecting turfgrass throughout the United States. Billbug larvae cause damage by feeding in stems, on roots, and on the crowns of turf, causing severe discoloration and eventual plant death. Monitoring efforts have focused on nondestructive pitfall sampling of ground-active billbug adults and on destructive sampling using soil cores for larval stages in the soil. Given the cryptic nature of the susceptible larval stages, billbugs are typically managed by preventive applications of long-residual, systemic insecticides, including neonicotinoids and anthranilic diamides. Despite knowledge of effective management practices including pest-resistant turf varieties, irrigation management, and microbial controls that contribute to an IPM approach, billbug management continues to rely heavily on prophylactic synthetic insecticides. This review will summarize the identification and biology of billbugs and strategies for their management. PMID:27065080

  14. BESOUROS COPRÓFAGOS (COLEOPTERA; SCARABAEIDAE COLETADOS EM PIRACICABA, SP

    Directory of Open Access Journals (Sweden)

    RODRIGUES S.R.

    1998-01-01

    Full Text Available Através do uso de armadilhas "pitfall" iscadas com massa fecal fresca de bovinos, realizou-se a coleta de besouros coprófagos (Coleoptera; Scarabaeidae, durante o período de 15 de abril de 1995 a 17 de fevereiro de 1996, em área de pastagem ao lado de confinamento de bovinos, em Piracicaba, SP. Coletou-se um total de 11 espécies distribuidas nos gêneros Aphodius, Ataenius, Trichillum, Eurysternus, Dichotomius e Canthon. Os besouros coprófagos de comportamento endocoprídeo representaram 72,73% das espécies coletadas, sendo estes os de maior ocorrência e a espécie Aphodius lividus parece ser a mais bem adaptada na área estudada.

  15. Walking stability of Rhyzopertha dominica (Fabricius, 1792) (Coleoptera: Bostrichidae).

    Science.gov (United States)

    Pires, E M; Nogueira, R M; Pina, D S; Manica, C L M; Faroni, L R A; Moreira, P S A

    2016-04-19

    Results obtained in studies can contribute to the advancement of science and innovative methods and techniques for developing practical activities. Reporting conditions that may restrict the implementation of research is critical to ensure the optimal development of further technical studies. The objective of this study was to assess the walking stability of R. dominica on a flat and smooth surface. The study was based on the determination of mortality, morphology and walking stability of the insect outside the grain mass, on a flat and smooth surface. Mortality of adults of this Coleoptera in conditions with and without food was similar, which explains the difficulty that this insect had for accessing the food source on the flat and smooth surface. The measurements of body length (BOL), width (BOW) and height (BOH) of R. dominica were compared with those of Tribolium castaneum (Coleoptera: Tenebrionidae), which showed good ability to walk in these conditions. This study indicated that the former presents lower BOL and BOW, and greater BOH than the second, and all these variables showed differences when analyzed simultaneously by means of the construction of multivariate morphometric indices (Width × Height, Length × Height and Height × Length × Width). These morphometric variables, together with the definition of the geometry most similar to the body shape, resulted in determination of the center of gravity (CG) and static rollover threshold (SRTgeom) for both species. Rhyzopertha dominica and T. castaneum presented CGs considered high and low, respectively, and together with the values obtained for SRTgeom, may justify that R. dominica can be considered a less stable species during movement, and presents greater risk of rollover on flat and smooth surfaces. PMID:27097085

  16. Track analysis of the Neotropical Entimini (Coleoptera: Curculionidae: Entiminae

    Directory of Open Access Journals (Sweden)

    Alberto Romo

    2011-09-01

    Full Text Available Track analysis of the Neotropical Entimini (Coleoptera, Curculionidae, Entiminae. Distributional patterns of the species belonging to the tribe Entimini from the Neotropical region were analyzed. Based on a track analysis of 22 species of Entimus, Rhigus, and Phaedropus, for which distributional data were available, two generalized tracks were found. One is located in northern Brazil, corresponding to the Amazonian subregion, and is determined by Phaedropus candidus and Rhigus speciosus. The other is located in southern Brazil, corresponding to the Parana subregion, and is determined by Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus, and R. tribuloides. The development of the Chacoan subregion is hypothesized to have been the dynamic vicariant event that fragmented the former Amazonian-Parana forest.Análisis de trazos de Entimini Neotropical (Coleoptera, Curculionidae, Entiminae. Se analizaron los patrones de distribución de la tribu Entimini en la región Neotropical. Con base en un análisis de los trazos de 22 especies de Entimus, Rhigus y Phaedropus, para los cuales se contaba con datos de distribución, se hallaron dos trazos generalizados. Uno se localiza en el norte de Brasil, en la subregión Amazónica, y está sustentado por Phaedropus candidus y Rhigus speciosus. El otro se localiza en el sur de Brasil, en la subregión Paranaense, y está sustentado por Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus y R. tribuloides. Se hipotetiza que el desarrollo de la subregión Chaqueña constituyó el evento de vicarianza dinámica que fragmentó el antiguo bosque Amazónico-Paranaense.

  17. Treatment of pityriasis versicolor with topical application of essential oil of Cymbopogon citratus (DC Stapf - therapeutic pilot study Tratamento de pitiríase versicolor com aplicação tópica do óleo essencial de Cymbopogon citratus (DC Stapf - estudo terapêutico piloto

    Directory of Open Access Journals (Sweden)

    Egberto Santos Carmo

    2013-06-01

    Full Text Available BACKGROUND: Pityriasis versicolor is a fungal infection caused by Malassezia spp. that has frequent relapses. OBJECTIVES: The main objective of this research was to perform phase I and II clinical studies, using formulations containing essential oil of Cymbopogon citratus in patients with pityriasis versicolor. METHODS: Phase I study included twenty volunteers to ascertain the safety of the formulations. In phase II, 47 volunteers randomly received essential oil formulations at 1.25 μL/mL concentration, for forty days. The shampoo should be applied three times a week and the cream twice a day. A control group in phase II, consisting of 29 volunteers, received the same formulations but with 2% ketoconazole as the active ingredient. RESULTS: No significant adverse events were observed in volunteers during Phase I. In Phase II, 30 (63.83% volunteers using essential oil and 18 (62.07% using ketoconazole remained until the end of the study. We observed a predominance of lesions in disseminated form, with M. sympodialis detected as the predominant agent identified in cultures. After 40 days of treatment, the rate of mycological cure was 60% (p FUNDAMENTOS: Pitiríase versicolor é uma micose causada pela Malassezia spp., e que apresenta frequentes recidivas. OBJETIVOS: Este trabalho objetivou realizar estudos clínicos de fase I e II, para essa patologia, com óleo essencial de Cymbopogon citratus. MÉTODOS: Na fase I, participaram vinte voluntários para averiguar a segurança das formulações. Na fase II, 47 voluntários receberam as formulações do óleo essencial a 1,25 μL/mL, as quais deveriam ser utilizadas por quarenta dias, sendo o xampu três vezes por semana e o creme duas vezes ao dia. Um grupo controle na fase II, constituído por 29 voluntários recebeu as mesmas formulações, com cetoconazol a 2%. RESULTADOS: Verificada a segurança das formulações com a finalização da fase I, onde nenhuma reação adversa significativa foi

  18. Bio-edafology of the Coleoptera order, in three Colombia Natural Regions

    International Nuclear Information System (INIS)

    The characterization of Coleoptera families is showed to three different Colombian Natural Regions. This is given in relation with biological and environmental factors, different vegetation covers and soil uses. In addition to it, susceptible Coleoptera taxa are determined when natural conditions are disturbed. Methodically, a literature subject research was made, and Barber and Berlesse traps were used to organism extractions from superficial and under superficial soil. Horizons diversity, riches and constancy index were determined to each family. Results show different diversity, riches and constancy values to each family, as much in each natural region, such as to each soil use

  19. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae)

    OpenAIRE

    Dilma Solange Napp; Ubirajara Ribeiro Martins

    2006-01-01

    Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae). Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae). The following new genus and species, all from Bolivia, are describ...

  20. Influence of growing location and cultivar on Rhyzopertha dominica (Coleoptera: Bostrichidae) and Sitophilus oryzae (Coleoptera: Curculionidae) infestation of rough rice

    Institute of Scientific and Technical Information of China (English)

    FRANK H. ARTHUR; RUSTY C. BAUTISTA; TERRENCE J. SIEBENMORGEN

    2007-01-01

    Long-grain rice cultivars Cocodrie, Wells, and XP 723 grown in three locations (Hazen, MO; Essex and Newport, AR, USA), and medium-grain rice cultivars Bengal and XP 713 grown in two locations (Jonesboro and Lodge Corner, AR, USA), were harvested and assayed for susceptibility to Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), the lesser grain borer, and Sitophilus oryzae (L.) (Coleoptera: Curculionidae), the rice weevil, on rice held at 27℃ 57% and 75% relative humidity (RH).Separate samples from the same harvest lots were also analyzed for the physical characteristics of brown rice yield, percentage whole kernels and kernel thickness. Progeny production and feeding damage of R. dominica were significantly different among long-grain cultivars within two of the three locations (P < 0.05), but not for location or RH (P ≥ 0.05), while progeny production of S. oryzae was different among cultivars, location, and RH (P < 0.05). On medium-grain rice, both cultivar and location were significant for progeny production of R. dominica, but not RH, while cultivar and RH were significant for progeny production of S. oryzae, but not location. On both rice types, feeding damage of R. dominica followed the same trends and was always strongly positively correlated with progeny production (P < 0.05), but for S. oryzae there were several instances in which progeny production was not correlated with feeding damage (P ≥ 0.05). Physical characteristics of both rice types were statistically significant (P < 0.01) but actual numerical differences were extremely small, and were generally not correlated with progeny production of either species. Results indicate that the location in which a particular rice cultivar is grown, along with its characteristics, could affect susceptibility of the rice to R. dominica and S. oryzae.

  1. Utility of morphological and molecular techniques for determination of paternity in two subspecies of Diabrotica undecimpunctata (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    An experiment was conducted to determine the paternity of F1 progeny using morphological and molecular methods in Diabrotica (Coleoptera: Chrysomelidae) subspecies: Diabrotica undecimpunctata howardi Barber, also known as spotted cucumber beetle and D. undecimpunctata undecimpunctata Mannerheim, als...

  2. Parasitylenchus sp. (Tylenchomorpha: Allantonematidae) parasitizing field populations of Harmonia axyridis (Coleoptera: Coccinellidae)

    DEFF Research Database (Denmark)

    Harding, Susanne; Poinar, George O. Jr.; Dimitrova, Desislava V.; Steenberg, Tove

    2011-01-01

    Adults of the invasive harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae), were found to be parasitized by nematodes (Tylenchomorpha: Allantonematidae) in Denmark. The nematodes were identified as Parasitylenchus sp. Major morphological characters of the nematodes did not differ si...

  3. Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae), information on a little known taxon from Colombia

    OpenAIRE

    Kippenhan, Mike

    2011-01-01

    Two specimens of Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae) in the Field Museum of Natural History offer additional information on the morphology and distribution of this rare species. One of the specimens, a female, is considered to be the first known specimen of this species.

  4. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae from Korea

    Directory of Open Access Journals (Sweden)

    Ik Je Choi

    2016-03-01

    Full Text Available A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  5. Patterns of tree species usage by long-horned beetles (Coleoptera: Cerambycidae) in Fiji

    Czech Academy of Sciences Publication Activity Database

    Waqa-Sakiti, H.; Stewart, A.; Čížek, Lukáš; Hodge, S.

    2014-01-01

    Roč. 68, č. 1 (2014), s. 57-64. ISSN 0030-8870 R&D Projects: GA ČR GAP504/12/1952 Institutional support: RVO:60077344 Keywords : Coleoptera Subject RIV: EH - Ecology, Behaviour Impact factor: 0.924, year: 2014 http://www.bioone.org/doi/pdf/10.2984/68.1.5

  6. An illustrated checklist of dung beetles (Coleoptera: Scarabaeinae from the Periyar Tiger Reserve, Kerala, India

    Directory of Open Access Journals (Sweden)

    Nithya Sathiandran

    2015-12-01

    Full Text Available An illustrated checklist of 36 species of dung beetles (Coleoptera: Scarabaeinae from the Periyar Tiger Reserve in the southern Western Ghats is presented.  Records of eight species endemic to the Western Ghats and a rare primitive old world dung beetle group, Ochicanthon nitidus (Paulian, from the forests of Periyar Tiger Reserve are provided. 

  7. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae) from Korea

    OpenAIRE

    Ik Je Choi; Jongok Lim; Jinyoung Park; Ji Hwan Park; Jong Kyun Park

    2016-01-01

    A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae) is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  8. Ground beetle (Coleoptera: Carabidae) assemblages in the Conservation Reserve Program crop rotation systems in Interior Alaska

    Science.gov (United States)

    Adult ground beetles (Coleoptera: Carabidae) abundance and diversity were documented on Conservation Research Program (CRP) agricultural lands in Delta Junction, Alaska (64ºN, 145º W). Twenty species were documented based on a total sample of 6,116 specimens collected during 2006 and 2007. Two speci...

  9. ESPECIES DE DRYOPHTHORINAE (COLEOPTERA: CURCULIONIDAE ASOCIADAS A PLÁTANO Y BANANO (Musaspp. EN COLOMBIA Dryophthorinae Species (Coleoptera: Curculionidae Associted to Platain and Banana crops (Musaspp. in Colombia

    Directory of Open Access Journals (Sweden)

    PAULA A SEPÚLVEDA-CANO

    Full Text Available Se presenta una sinopsis de los escarabajos de la subfamilia Dryophthorinae (Coleoptera: Curculionidae asociados a cultivos de plátano y banano en Colombia. Adicionalmente se ofrecen claves ilustradas para las especies del país. Se registran seis especies asociadas a dichos cultivos: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.This synopsis is about beetle&#’;s subfamily Dryophthorinae (Coleoptera: Curculionidae associated to plantain and banana crops. Additionally keys illustrated for the species of the country are offered. Six species associated to these cultures are registered: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.

  10. Chaves para a identificação dos principais Coleoptera (Insecta associados com produtos armazenados Keys for the identification of Coleoptera (Insecta associated with stored products

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Valle da Silva Pereira

    2001-03-01

    Full Text Available An illustrated key to identify nine families of Coleoptera commonly found in stored products is presented. Keys for the identification of Anobiidae [Lasioderma serricorne (Fabricius, 1792, Stegobium paniceum (Linnaeus, 1761], Bruchidae [Acanthoscelides obtectus (Say, 1831, Zabrotes subfasciatus (Boheman, 1833], Curculionidae [Sitophilus oryzae (Linnaeus, 1763, S. zeamais Motschulsky, 1885], Silvanidae [Ahasverus advena (Waltl, 1832, Cathartus quadricollis (Guérin, 1892, Oryzaephilus mercator (Fauvel, 1889, O. surinamensis (Linnaeus, 1758] and Tenebrionidae [Gnathocerus cornutus (Fabricius, 1798, Tribolium castaneum (Herbst, 1797, T. confusum du Val, 1868] are also provided. These keys cover the most frequent Coleoptera found in stored products, specially grains, and are to the adult stage only. Illustrations of external morphology and general characteristics are provided for each species reported.

  11. Multistate characters and diet shifts: evolution of Erotylidae (Coleoptera).

    Science.gov (United States)

    Leschen, Richard A B; Buckley, Thomas R

    2007-02-01

    The dominance of angiosperms has played a direct role in the diversification of insects, especially Coleoptera. The shift to angiosperm feeding from other diets is likely to have increased the rate of speciation in Phytophaga. However, Phytophaga is only one of many hyperdiverse lineages of beetles and studies of host-shift proliferation have been somewhat limited to groups that primitively feed on plants. We have studied the diet-diverse beetle family Erotylidae (Cucujoidea) to determine if diet is correlated with high diversification rates and morphological evolution by first reconstructing ancestral diets and then testing for associations between diet and species number and diet and ovipositor type. A Bayesian phylogenetic analysis of morphological data that was previously published in Leschen (2003, Pages 1-108 in Fauna of New Zealand, 47; 53 terminal taxa and 1 outgroup, 120 adult characters and 1 diet character) yielded results that are similar to the parsimony analyses of Leschen (2003). Ancestral state reconstructions based on Bayesian and parsimony inference were largely congruent and both reconstructed microfungal feeding (the diet of the outgroup Biphyllidae) at the root of the Erotylidae tree. Shifts among microfungal, saprophagous, and phytophagous diets were most frequent. The largest numbers of species are contained in lineages that are macrofungal feeders (subfamily Erotylinae) and phytophagous (derived Languriinae), although the Bayesian posterior predictive tests of character state correlation were unable to detect any significant associations. Ovipositor morphology correlated with diet (i.e., acute forms were associated with phytophagy and unspecialized forms were associated with a mixture of diets). Although there is a general trend to increased species number associated with the shift from microfungal feeding to phytophagy (based on character mapping and mainly restricted to shifts in Languriinae), there is a large radiation of taxa feeding on

  12. Biomassa de Dynastinae (Coleoptera, Scarabaeidae fototácticos: um ensaio biogeográfico / Biomass of phototactic Dynastinae (Coleoptera, Scarabaeidae: a biogeographical assay

    Directory of Open Access Journals (Sweden)

    Paulo Jorge Riehs

    2006-04-01

    Full Text Available São apresentados e analisados valores da biomassa de Dynastinae (Coleoptera, Scarabaeidae fototácticos coletados em seis localidades do Leste e Centro-Oeste do Paraná, sul do Brasil, demonstrando-se as diferenças entre as diversas comunidades. Os dados obtidos no Paraná são correlacionados com os de Santa Maria, Rio Grande do Sul.

  13. Molecular Markers Detect Cryptic Predation on Coffee Berry Borer (Coleoptera: Curculionidae) by Silvanid and Laemophloeid Flat Bark Beetles (Coleoptera: Silvanidae, Laemophloeidae) in Coffee Beans.

    Science.gov (United States)

    Sim, Sheina B; Yoneishi, Nicole M; Brill, Eva; Geib, Scott M; Follett, Peter A

    2016-02-01

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is a serious pest of coffee worldwide. It was first detected in Hawai'i in 2010. Two predatory beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and Leptophloeus sp. (Coleoptera: Laemophloeidae), have been observed in H. hampei-infested coffee. Under laboratory conditions, colony-reared C. quadricollis and Leptophloeus sp. prey upon all life stages of H. hampei. However, the H. hampei life cycle occurs almost exclusively within a coffee bean obscured from direct observation. Thus, it is unknown if C. quadricollis and Leptophloeus sp. consume H. hampei as prey in the wild. To demonstrate predation of H. hampei by C. quadricollis and Leptophloeus sp., a molecular assay was developed utilizing species-specific primers targeting short regions of the mitochondrial COI gene to determine species presence. Using these primers, wild C. quadricollis and Leptophloeus sp. were collected and screened for the presence of H. hampei DNA using PCR. Analysis of collections from five coffee farms revealed predation of C. quadricollis and Leptophloeus sp. on H. hampei. Further laboratory testing showed that H. hampei DNA could be detected in predators for as long as 48 h after feeding, indicating the farm-caught predators had preyed on H. hampei within 2 d of sampling. This study demonstrates the utility of molecular markers for the study of the ecology of predators and prey with cryptic behavior, and suggests C. quadricollis and Leptophloeus sp. might be useful biocontrol agents against H. hampei. PMID:26487745

  14. Suscetibilidade de Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae ao enxofre Susceptibility of Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae to sulfur

    Directory of Open Access Journals (Sweden)

    José Roberto Gonçalves

    2007-08-01

    Full Text Available As criações de Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae são freqüentemente infestadas pelo ácaro Acarophenax lacunatus (Cross e Krantz (Prostigmata: Acarophenacidae. O objetivo deste trabalho foi avaliar doses de enxofre, acaricida eficaz contra A. lacunatus, não-prejudiciais ao desenvolvimento de R. dominica. As unidades experimentais foram constituídas de placas de Petri contendo 30 g de grãos de trigo infestados com 30 adultos de R. dominica. Os tratamentos consistiram na utilização de doses de enxofre sobre os grãos, correspondentes a 0,0; 0,6; 0,9; 1,2; 1,5; 3,0; 6,0; 12,0; 24,0 e 48,0mg i a g-1, em dez repetições. As unidades experimentais foram armazenadas por 60 dias a 30±1°C, 60±5% UR e escotofase de 24h. O desenvolvimento de R. dominica foi afetado pela utilização de doses de enxofre maiores que 3,0mg i a g-1.The laboratory rearing of Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae is frequently infested by the parasite mite Acarophenax lacunatus (Cross and Krantz (Prostigmata: Acarophenacidae. This study was aimed at evaluating the sulfur doses, an effective acaricide against A. lacunatus, not harmful to the development of with R. dominica. The experimental units were Petri dishes containing 30g of whole wheat grains powdered with the different doses of the sulfur (0.0, 0.6, 0.9, 1.2, 1.5, 3.0, 6.0, 12.0, 24.0 and 48.0mg a i g-1 infested with 30 adults of R. dominica, in ten replicates. All treatments were maintained under controlled conditions (30±1°C, 60±5% r h and 24h scotophase for 60 days after the insect infestation. Sulfur doses higher than 3.0mg a i g-1 negatively affected R. dominica development.

  15. 彩绒革盖菌漆酶产酶条件研究%Research on the Conditions for Laccase Production from Coriolus versicolor

    Institute of Scientific and Technical Information of China (English)

    王宜磊; 刘兴坦

    2001-01-01

    本文研究了碳源、氮源、愈创木酚、香兰素及培养条件对漆酶分泌的影响;结果表明,淀粉作碳源、干酪素作氮源有利于漆酶的分泌,适宜浓度的愈创木酚和香兰素等对漆酶的产生有一定的作用;pH在3.0~8.0的范围内对漆酶的分泌影响差别不大,培养温度、接种量、通气量对漆酶的分泌有较大影响。%Effects of carbon source, nitrogen source, o-methoxyphenol, vanillin and culture conditions on laccase production from Coriolus versicolor were studied. The results showed that starch as carbon source, and casein and soybean powder as nitrogen source, o-methoxyphenol and vanillin of suitable concentration had good influence on laccase production. There were a little difference between effects of pH 3.0~8.0 in the medium on laccase production, but the culture temperature, inoculation amount and ventilation volume had greater influence on laccase production.

  16. Enhanced biodegradation of antibiotic combinations via the sequential treatment of the sludge resulting from pharmaceutical wastewater treatment using white-rot fungi Trametes versicolor and Bjerkandera adusta.

    Science.gov (United States)

    Aydin, Sevcan

    2016-07-01

    While anaerobic treatment is capable of treating pharmaceutical wastewater and removing antibiotics in liquid phases, solid phases may still contain significant amounts of antibiotics following this treatment. The main goal of this study was to evaluate the use of white-rot fungi to remove erythromycin, sulfamethoxazole, and tetracycline combinations from biosolids. The degradation potential of Trametes versicolor and Bjerkandera adusta was evaluated via the sequential treatment of anaerobic sludge. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses were used to identify competition between the autochthonous microbial communities and white-rot fungi. Solid-phase treatment using white-rot fungi substantially reduced antibiotic concentrations and toxicity in sludge. According to PCR-DGGE results, there is an association between species of fungus and antibiotic type as a result of the different transformation pathways of fungal strains. Fungal post-treatment of sludge represents a promising method of removing antibiotic combinations, therefore holding a significant promise as an environmentally friendly means of degrading the antibiotics present in sludge. PMID:27033714

  17. In vivo and in vitro anti-tumor and anti-metastasis effects of Coriolus versicolor aqueous extract on mouse mammary 4T1 carcinoma.

    Science.gov (United States)

    Luo, Ke-Wang; Yue, Grace Gar-Lee; Ko, Chun-Hay; Lee, Julia Kin-Ming; Gao, Si; Li, Long-Fei; Li, Gang; Fung, Kwok-Pui; Leung, Ping-Chung; Lau, Clara Bik-San

    2014-01-01

    Coriolus versicolor (CV), a medicinal mushroom widely consumed in Asian countries, has been demonstrated to be effective in stimulation of immune system and inhibition of tumor growth. The present study aimed to investigate the anti-tumor and anti-metastasis effects of CV aqueous extract in mouse mammary carcinoma 4T1 cells and in 4T1-tumor bearing mouse model. Our results showed that CV aqueous extract (0.125-2 mg/ml) did not inhibit 4T1 cell proliferation while the non-cytotoxic dose of CV extract (1-2 mg/ml) significantly inhibited cell migration and invasion (pbreast cancer-induced bone destruction as the bone volume was significantly increased. On the other hand, CV aqueous extract treatments resulted in remarkable immunomodulatory effects, which was reflected by the augmentation of IL-2, 6, 12, TNF-α and IFN-γ productions from the spleen lymphocytes of CV-treated tumor-bearing mice. In conclusion, our results demonstrated for the first time that the CV aqueous extract exhibited anti-tumor, anti-metastasis and immunomodulation effects in metastatic breast cancer mouse model, and could protect the bone from breast cancer-induced bone destruction. These findings provided scientific evidences for the clinical application of CV aqueous extract in breast cancer patients. PMID:24856767

  18. Immobilization of the laccases from trametes versicolor and streptomyces coelicolor on single-wall carbon nanotube electrodes: a molecular dynamics study

    Energy Technology Data Exchange (ETDEWEB)

    Trohalaki, S. [Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, OH (United States); General Dynamics Information Technology, Dayton, OH (United States); Pachter, R. [Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, OH (United States); Luckarift, H.R. [Materials and Manufacturing Directorate, Air Force Research Laboratory, Tyndall Air Force Base, FL (United States); Universal Technology Corporation, Dayton, OH (United States); Johnson, G.R. [Materials and Manufacturing Directorate, Air Force Research Laboratory, Tyndall Air Force Base, FL (United States)

    2012-08-15

    In this work, we investigate the immobilization of laccases from Trametes versicolor (TvL) and the small laccase (SLAC) from Streptomyces coelicolor on single-wall carbon nanotube (SWCNT) surfaces. SLAC may potentially offer improved adsorption on the electrode, thus improving bioelectrocatalytic activity via direct electron transfer (DET). Laccase immobilization on SWCNTs is achieved non-covalently with a molecular tether (1-pyrene butanoic acid, succinimidyl ester) that forms an amide bond with an amine group on the laccase surface while the pyrene coordinates to the SWCNT by {pi}-{pi} stacking. In our approach, density functional theory calculations were first used to model the interaction energies between SWCNTs and pyrene to validate an empirical force field, thereafter applied in molecular dynamics (MD) simulations. In the simulated models, the SWCNT was placed near the region of the (type 1) Cu(T1) atom in the laccases, and in proximity to other regions where adsorption seems likely. Calculated interaction energies between the SWCNTs and laccases and distances between the SWCNT surface and the Cu(T1) atom have shown that SWCNTs adsorb more strongly to SLAC than to TvL, and that the separation between the SWCNTs and Cu(T1) atoms is smaller for SLAC than for TvL, having implications for improved DET. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  19. A Ca-alginate particle co-immobilized with Phanerochaete chrysosporium cells and the combined cross-linked enzyme aggregates from Trametes versicolor.

    Science.gov (United States)

    Li, Yanchun; Wang, Zhi; Xu, Xudong; Jin, Liqiang

    2015-12-01

    For improving stability of immobilized white-rot fungus to treat various effluents, Phanerochaete chrysosporium cells and the combined cross-link enzyme aggregates (combi-CLEAs) prepared from Trametes versicolor were co-immobilized into the Ca-alginate gel particles in this paper. The activity yields of obtained combi-CLEAs were 42.7% for lignin peroxidases (LiPs), 31.4% for manganese peroxidases (MnPs) and 40.4% for laccase (Lac), respectively. And their specific activities were 30.2U/g as combi-CLEAs-LiPs, 9.5 U/g as combi-CLEAs-MnPs and 28.4 U/g as combi-CLEAs-Lac. Further, the present of the combi-CLEAs in the particles extremely improved their ability to degrade the dyes. Compared to the immobilized Ph. chrysosporium without the combi-CLEAs, the co-immobilized particles enhanced the decolorized rate of Acid Violet 7 (from 45.2% to 93.4%) and Basic Fuchsin (from 12.1% to 67.9%). In addition, the addition of the combi-CLEAs improved the adaptability of the white-rot fungal particles to adverse environmental conditions. PMID:26413897

  20. The complete mitogenome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae).

    Science.gov (United States)

    Nan, Xiaoning; Wei, Cong; He, Hong

    2016-05-01

    The complete mitochondrial genome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae) were reconstructed from whole-genome Illumina Hiseq 2000 sequencing data with an average coverage of 1406.7X. The circular genome is 15,122 bp in length, consisting of 13 protein-coding genes (PCGs), 21 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and one D-loop or control region. The tRNA-Ile gene was not found in the mitochondrial genome, as is identical to two other curculionidae species, i.e. Sphenophorus sp. (GU176342) and Naupactus xanthographus (GU176345). All PCGs initiated with ATN codons, except for the ND1 started with TTG. Two PCGs (COI and ND4) have an incomplete stop codon T. Two PCGs (ND4L and ND1) harbor the stop codon TAG, while all other PCGs terminated with the TAA codon. The nucleotide composition is highly asymmetric (38.7% A, 14.4% C, 9.2% G and 37.8% T) with an overall AT content of 76.5%. PMID:25427809

  1. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae) *

    Science.gov (United States)

    Petitpierre, Eduard

    2011-01-01

    Abstract Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xyp and XYp clearly prevailing (79.0%), but with the XO well represented too (19.75%). The modal haploid number for chrysomelines is n = 12 (34.2%) although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6%) and in subtribe Chrysomelina n = 17 (65.7%). Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74) has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry. PMID:22303104

  2. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae).

    Science.gov (United States)

    Petitpierre, Eduard

    2011-01-01

    Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xy(p) and XY(p) clearly prevailing (79.0%), but with the XO well represented too (19.75%). The modal haploid number for chrysomelines is n = 12 (34.2%) although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6%) and in subtribe Chrysomelina n = 17 (65.7%). Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74) has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry. PMID:22303104

  3. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae

    Directory of Open Access Journals (Sweden)

    Eduard Petitpierre

    2011-12-01

    Full Text Available Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xyp and XYp clearly prevailing (79.0%, but with the XO well represented too (19.75%. The modal haploid number for chrysomelines is n = 12 (34.2% although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6% and in subtribe Chrysomelina n = 17 (65.7%. Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74 has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry.

  4. Plant compounds insecticide activity against Coleoptera pests of stored products

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Marcio Dionizio; Picanco, Marcelo Coutinho; Guedes, Raul Narciso Carvalho; Campos, Mateus Ribeiro de; Silva, Gerson Adriano; Martins, Julio Claudio [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Biologia Animal]. E-mail: marcio.dionizio@gmail.com; picanco@ufv.br; guedes@ufv.br; mateusc3@yahoo.com.br; agronomiasilva@yahoo.com.br

    2007-07-15

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on R. dominica was evaluated. Among them, only hexane extract of A. conyzoides showed insecticide activity; the hexane extract of this species was successively fractionated by silica gel column chromatography, for isolation and purification of the active compounds. Compounds 5,6,7,8,3',4',5'-heptamethoxyflavone; 5,6,7,8,3'-pentamethoxy-4',5'-methilenedioxyflavone and coumarin were identified. However, only coumarin showed insecticide activity against three insect pests (LD{sub 50} from 2.72 to 39.71 mg g{sup -1} a.i.). The increasing order of insects susceptibility to coumarin was R. dominica, S. zeamais and O. surinamensis. (author)

  5. Economic analysis of emerald ash borer (Coleoptera: Buprestidae) management options.

    Science.gov (United States)

    Vannatta, A R; Hauer, R H; Schuettpelz, N M

    2012-02-01

    Emerald ash borer, Agrilus planipennis (Fairmaire) (Coleoptera: Buprestidae), plays a significant role in the health and extent of management of native North American ash species in urban forests. An economic analysis of management options was performed to aid decision makers in preparing for likely future infestations. Separate ash tree population valuations were derived from the i-Tree Streets program and the Council of Tree and Landscape Appraisers (CTLA) methodology. A relative economic analysis was used to compare a control option (do-nothing approach, only removing ash trees as they die) to three distinct management options: 1) preemptive removal of all ash trees over a 5 yr period, 2) preemptive removal of all ash trees and replacement with comparable nonash trees, or 3) treating the entire population of ash trees with insecticides to minimize mortality. For each valuation and management option, an annual analysis was performed for both the remaining ash tree population and those lost to emerald ash borer. Retention of ash trees using insecticide treatments typically retained greater urban forest value, followed by doing nothing (control), which was better than preemptive removal and replacement. Preemptive removal without tree replacement, which was the least expensive management option, also provided the lowest net urban forest value over the 20-yr simulation. A "no emerald ash borer" scenario was modeled to further serve as a benchmark for each management option and provide a level of economic justification for regulatory programs aimed at slowing the movement of emerald ash borer. PMID:22420272

  6. 杂色龙虾线粒体基因组全序列的测定与分析%Sequencing and analysis of the complete mitochondrial geno-me of Panulirus versicolor

    Institute of Scientific and Technical Information of China (English)

    刘皓; 姚维; 刘海情; 刘楚吾; 刘丽

    2014-01-01

    为了解杂色龙虾(Panulirus versicolor)线粒体基因组多态位点及遗传变异,对龙虾类的分类研究奠定基础,作者通过常规 PCR步移扩增法获得杂色龙虾线的线粒体基因组全序列,分析了其线粒体基因组的基本特征以及基因排列情况,并结合中国龙虾(Panulirus stimposni)、波纹龙虾(Panulirus homa-rus)线粒体基因组全序列,综合分析了龙虾属线粒体编码基因多态位点及基因变异状况。结果表明:杂色龙虾线粒体基因组全序列为15700 bp,由13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和 D-loop区组成,保持了泛甲壳动物线粒体基因组的原始排列。杂色龙虾线粒体 DNA 的碱基组成具有AT偏好性, A+T含量为67%,4种碱基的含量分别为(A=34.6%, T=32.1%, C=20.5%, G=12.8%)。预测了杂色龙虾18个tRNA以及2个rRNA的二级结构。龙虾属线粒体基因组中的nad 2、nad 5基因以及D-loop 区的多态位点比例较高,适合作为分子标记用于龙虾类系统进化关系、种内多态性以及遗传分化等方面的研究。%The complete sequence of Panulirus versicolor mitochondrial genome was determined by using conventional PCR and conserved primers walking approaches. The basic characteristics and gene arrangement of P. versicolor mito-chondrial genome were analyzed. Combined with the mitochondrial genome of P. stimposni and P. homarus, the mito-chondrial genes encoding polymorphic loci and gene mutation of Panulirus were also analyzed. The results show that the entire mitochondrial genome of P. versicolor is 15700 bp in length, consisting of 13 protein-coding genes, 22 tRNA genes and 2 rRNA genes and D-loop control area, which maintained the original arrangement of crustacean animal mito-chondrial genomes. The composition of P. versicolor mitochondrial DNA base has AT preference, with 67%A+T con-tent and the content of A, T, C, and G was 34.6%, 32.1%, 20.5,%and 12.8%respectively. The secondary

  7. Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae

    Directory of Open Access Journals (Sweden)

    Elias Soares Gomes

    2014-06-01

    Full Text Available Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae. Coleopterans of the family Melolonthidae comprise a large group of species that feed on different food sources, including plant roots, stems, and leaves, in addition to plant materials at different decomposition stages. Several species are found in the genus Leucothyreus, occurring in different regions of Brazil, including the various biomes in the country. Information on the biology of species of the genus Leucothyreus is scarce, therefore, we conducted studies on the biological aspects of Leucothyreus ambrosius Blanchard, 1850. The period of adult occurrence was determined with a light trap installed between a cropped and pasture area in the municipality of Aquidauana, Mato Grosso do Sul State, Brazil. Adults collected in the field were used to form insect pairs and the studies were initiated in the entomology laboratory as the adults began ovipositing. Adults were observed flying in the field from October to December. Eggs were obtained as pairs were formed and a colony was established, the embryonic period lasting 14.6 days on average. The larval period in the 1st instar lasted 21.6 days, in the 2nd instar 19.6 days, and in the 3rd instar, 85.6 days. The head capsule width was 1.48 mm in the 1st instar, 2.44 mm in the 2nd, and 3.83 mm in 3rd larval instar. The pupal stage had an average duration of 35.5 days. The egg to adult period lasted 173.3 days. Morphometric information for the larval and adult stages is presented in this study.

  8. Contributions to the knowledge of Atlantic Canadian Histeridae (Coleoptera

    Directory of Open Access Journals (Sweden)

    Christopher Majka

    2008-09-01

    Full Text Available New records of Histeridae from Atlantic Canada are reported. Three species are newly recorded from Prince Edward Island and two from New Brunswick, one of which, the introduced Palearctic Atholus bimaculatus (Linnaeus, is newly recorded from Atlantic Canada as a whole. These new records increase the known histerid fauna of the region to 37 species, 30 native and 7 introduced ones. The regional zoogeography of the Histeridae is examined focusing on differences between the faunal composition of the various provinces and the possible reasons responsible for these. The island faunas of Cape Breton, Prince Edward Island, and Newfoundland are examined. All have reduced faunas in comparison with the mainland perhaps as a result of island-associated diminutions, an area effect, a paucity of collecting, or a combination of these factors. Those of Cape Breton and Prince Edward Island are proportionately similar to those of other families of Coleoptera, whereas that of Newfoundland (only 10% of the mainland fauna is significantly less, a circumstance which deserves further investigation. Seven species of introduced histerids have been recorded in the region. The average dates of first detection of these species are much later than the earliest records of these species in North America and comparatively later than is the case with other suites of introduced species in the Staphylinidae and Carabidae, perhaps as a result of the sparse attention the Histeridae have historically received by coleopterists in the region. Most of the introduced histerids are known to be synanthropic and may have been introduced to the region association with the importation of livestock and materials related to animal husbandry. The Histeridae of the region largely fall into one of several trophic guilds: coastal species and those associated with beach-drift material; species associated with bird nests; species associated with mammal nests; myrmecophilus species; saproxylic

  9. Biocontrol of larval mosquitoes by Acilius sulcatus (Coleoptera: Dytiscidae

    Directory of Open Access Journals (Sweden)

    Banerjee Siddhartha S

    2008-10-01

    Full Text Available Abstract Background Problems associated with resistant mosquitoes and the effects on non-target species by chemicals, evoke a reason to find alternative methods to control mosquitoes, like the use of natural predators. In this regard, aquatic coleopterans have been explored less compared to other insect predators. In the present study, an evaluation of the role of the larvae of Acilius sulcatus Linnaeus 1758 (Coleoptera: Dytiscidae as predator of mosquito immatures was made in the laboratory. Its efficacy under field condition was also determined to emphasize its potential as bio-control agent of mosquitoes. Methods In the laboratory, the predation potential of the larvae of A. sulcatus was assessed using the larvae of Culex quinquefasciatus Say 1823 (Diptera: Culicidae as prey at varying predator and prey densities and available space. Under field conditions, the effectiveness of the larvae of A. sulcatus was evaluated through augmentative release in ten cemented tanks hosting immatures of different mosquito species at varying density. The dip density changes in the mosquito immatures were used as indicator for the effectiveness of A. sulcatus larvae. Results A single larva of A. sulcatus consumed on an average 34 IV instar larvae of Cx. quinquefasciatus in a 24 h period. It was observed that feeding rate of A. sulcatus did not differ between the light-on (6 a.m. – 6 p.m., and dark (6 p.m. – 6 a.m. phases, but decreased with the volume of water i.e., space availability. The prey consumption of the larvae of A. sulcatus differed significantly (P A. sulcatus larvae, while with the withdrawal, a significant increase (p A. sulcatus in regulating mosquito immatures. In the control tanks, mean larval density did not differ (p > 0.05 throughout the study period. Conclusion the larvae of the dytiscid beetle A. sulcatus proved to be an efficient predator of mosquito immatures and may be useful in biocontrol of medically important mosquitoes.

  10. Using malaise traps to sample ground beetles (Coleoptera: Carabidae).

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D., James L. Hanula, and Scott Horn

    2005-01-01

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  11. Using malaise traps to sample ground beetles (Coleoptera. Carabidae)

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Hanula, James L. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Horn, Scott [USDA Forest Service, Savannah River, New Ellenton, SC (United States)

    2012-04-02

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  12. Summarization of Researches on Chemical Compositions and Pharmacological Activities of Coriolus versicolor%云芝的化学成分及药理活性研究概况

    Institute of Scientific and Technical Information of China (English)

    李玲艳; 包海鹰

    2011-01-01

    The article has carried on the summarization of chemical compositions and pharmacological activities of Coriolus versicolor through document retrieving.Present studies have found that Coriolus versicolor contains 29 compounds,such as steroids,triterpenes,organic acids,alkaloids,Glucitols,proteins,carbohydrates,glycopeptides.Scholars has also found Coriolus versicolor contains many other compounds:ECMM,pepsin inhibitor,Elicitor,iron,conglutinated chelating agents,cyclopeptides,18 kinds of essential amino acids,VB1,VB2,VB6 and 10 more human body′s essential activitied microelements.Coriolus versicolor has such pharmacological actions as anti-tumor,analgesia,treating Chronic Hepatitis B,norblood fat,antiatherosclerosis,antioxi-dation,antiaging,benefiting vital energy and antipyresis,antimicrobial activities in vitro.%通过文献检索对云芝的化学成分及药理活性方面进行了综述。研究发现云芝含甾体类、三萜类、有机酸类、生物碱类、葡糖醇类、蛋白质类、糖类、糖肽类等29个化合物,还含胞外黏性物质(ECMM)、胃蛋白酶抑制剂、真菌激发子、铁黏合螯合剂、环肽、18种人体所需的氨基酸和维生素B1、B2、B6及铜、铁、钾、锌等10多种人体所需的活性微量元素等其他类化合物。云芝具有抗肿瘤、镇痛、治疗慢性乙型肝炎、降血脂和抗动脉粥样硬化、抗氧化、抗衰老、益气解毒、体外抗微生物活性等作用。

  13. Differential anti-tumor activity of coriolus versicolor (Yunzhi) extract through p53- and/or Bcl-2-dependent apoptotic pathway in human breast cancer cells.

    Science.gov (United States)

    Ho, Cheong-Yip; Kim, Chi-Fai; Leung, Kwok-Nam; Fung, Kwok-Pui; Tse, Tak-Fu; Chan, Helen; Lau, Clara Bik-San

    2005-06-01

    Coriolus versicolor (CV), also called Yunzhi, has been demonstrated to exert anti-tumor effects on various types of cancer cells, but the underlying mechanism has not been fully elucidated. The present study aimed to evaluate the in vitro anti-tumor activity of a standardized aqueous ethanol extract prepared from CV on four breast cancer cell lines using MTT assay, and test whether the mechanism involves apoptosis induction and modulation of p53 and Bcl-2 protein expressions using cell death detection ELISA, p53 and Bcl-2 ELISAs respectively. Our results demonstrated that the CV extract dose-dependently suppressed the proliferation of three breast tumor cell lines, with ascending order of IC50 values: T-47D, MCF-7, MDA-MB-231, while BT-20 cells were not significantly affected. Tumoricidal activity of the CV extract was found to be comparable to a chemotherapeutic anti-cancer drug, mitomycin C. Nucleosome productions in apoptotic MDA-MB-231, MCF-7 and T-47D cells were significantly augmented in a time-dependent manner and paralleled the anti-proliferative activity of CV extract. Expression of p53 protein was significantly upregulated only in T-47D cells treated with the CV extract in a dose- and time-dependent fashion, but not in MCF-7 (except at 400 mug/ml after 16 h) and MDA-MB-231 cells. The CV extract significantly induced a dose-dependent downregulation of Bcl-2 protein expression in MCF-7 and T-47D cells, but not in MDA-MB-231 cells. These results suggested that apoptosis induction, differentially dependent of p53 and Bcl-2 expressions, might be the possible mechanism of CV extract-mediated cytotoxicity in human breast cancer cells in vitro. PMID:15908782

  14. Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT on human leukemia HL-60 cells

    Directory of Open Access Journals (Sweden)

    Jiang Pingping

    2010-04-01

    Full Text Available Abstract Background Polysaccharopeptide (PSP from Coriolus versicolor (Yunzhi is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT. Methods We used bivariate bromodeoxyuridine/propidium iodide (BrdUrd/PI flow cytometry analysis to measure the relative movement (RM of the BrdUrd positively labeled cells and DNA synthesis time (Ts on the HL-60 cell line. We used annexin V/PI flow cytometry analysis to quantify the viable, necrotic and apoptotic cells. The expression of cyclin E and cyclin B1 was determined with annexin V/PI flow cytometry and western blotting. Human peripheral blood mononuclear cells were used to test the cytotoxicity of PSP and CPT. Results PSP reduced cellular proliferation; inhibited cells progression through both S and G2 phase, reduced 3H-thymidine uptake and prolonged DNA synthesis time (Ts in HL-60 cells. PSP-pretreated cells enhanced the cytotoxicity of CPT. The sensitivity of cells to the cytotoxic effects of CPT was seen to be the highest in the S-phase and to a small extent of the G2 phase of the cell cycle. On the other hand, no cell death (measured by annexin V/PI was evident with the normal human peripheral blood mononuclear cells with treatment of either PSP or CPT. Conclusion The present study shows that PSP increases the sensitization of the HL-60 cells to undergo effective apoptotic cell death induced by CPT. The pattern of sensitivity of cancer cells is similar to that of HL-60 cells. PSP rapidly arrests and/or kills cells in S-phase and did not interfere with the anticancer action of CPT. PSP is a potential adjuvant to treat human leukemia as rapidly proliferating tumors is characterized by a high proportion of S-phase cells.

  15. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae

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    Dilma Solange Napp

    2006-01-01

    Full Text Available Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae. Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae. The following new genus and species, all from Bolivia, are described in Compsocerini: Ecoporanga gen. nov., type species E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., from Santa Cruz, and Ethemon iuba sp. nov. from Cochabamba.

  16. Una especie nueva de Trechisibus de la Argentina (Coleoptera: Carabidae A new species of Trechisibus from Argentina (Coleoptera: Carabidae

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    Sergio Roig-Juñent

    2005-12-01

    Full Text Available RESUMEN. Las Trechini constituyen una tribu de Carabidae distribuida ampliamente en la región Andino Patagónica. A pesar de que existen varias revisiones parciales de esta tribu para la Argentina y Chile, es muy común hallar especies nuevas, debido sobre todo a la prospección de áreas no exploradas. En este aporte se describe una especie nueva del género Trechisibus Jeannel del Cerro Nevado (Mendoza, Argentina. Por sus características morfológicas pertenece al grupo de especies depressus. Se describe e ilustra el adulto de esta especie nueva, se provee una clave para la identificación de las especies del grupo depressus y se discuten algunos aspectos de la distribución de estas especies.ABSTRACT. Trechini is a tribe of Carabidae (Coleoptera widely distributed in the Andean Patagonian region in South America. In spite of several partial revisions of the tribe for Argentina and Chile, it is very common to find new species due to the research of unexplored areas. In the present paper, a new species of the genus Trechisibus Jeannel from the Cerro Nevado (Mendoza, Argentina is described. Based on its morphological features the new species is considered as a member of the depressus group. The adult of the new species is described and illustrated, a key for the identification of the species of the depressus group is provided, and some aspects of the distribution of the group are discussed.

  17. Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil

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    Renata C. Campos; Malva I. Medina Hernández

    2013-01-01

    Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil. The beetles of the subfamily Scarabaeinae are important organisms that participate in the cycle of decomposition, especially in tropical ecosystems. Most species feed on feces (dung) or carcasses (carrion) and are associated with animals that produce their food resources. Dung beetles are divided into three functional groups: rollers, tunnelers and dwellers. This present work aims to study the ...

  18. Species of beetles (Coleoptera; Scarabaeidae associated to banana (Musa spp. in Ceballos, Ciego de Avila, Cuba

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    Maria Luisa Sisne Luis

    2016-03-01

    Full Text Available A white light trap was placed in bananas plantations, according to Sisne, 2009 and MINAG, 1985, in the Citric enterprise of Ciego de Ávila during the period between May and July of 2010 with the objective of determining the composition of genus and species of the order Coleoptera family Scarabaeidae associated to the agroecosystem. The species Cyclocephala cubana Chapin, Phyllophaga puberula Duval, and Phyllophaga patruelis Chev. are associated to bananas crops in these areas.

  19. Ecological impact of entomopathogenic nematodes used to control the large pine weevil, Hylobius abietis (Coleoptera: Curculionidae)

    OpenAIRE

    Harvey, Christopher D.

    2010-01-01

    The large pine weevil (Hylobius abietis; Coleoptera: Curculionidae) is one of the most economically damaging pests in forestry across Northern Europe. Its larvae develop in coniferous tree stumps on clearfell sites and adult weevils feed on seedlings that are replanted on these sites, causing substantial mortality. As the drive towards achieving the sustainable management of natural resources increases, biological control agents are being considered as an alternative to chemical ...

  20. Otiorhynchus spp. (Coleoptera: Curculionidae) as pests in horticulture: genetics and management options with entomopathogenic fungi

    OpenAIRE

    Hirsch, Jacqueline

    2012-01-01

    Worldwide, weevils of the genus Otiorhynchus (Coleoptera: Curculionidae) cause damage with detrimental economic effects to many horticultural crops due to the root feeding of their larvae as well as foliage feeding of their adults. Aside from the black vine weevil Otiorhynchus sulcatus, which is the best-known pest within this genus, numerous other Otiorhynchus species have been increasingly recognized as pests in recent years. Nocturnal adult weevils and soil-inhibiting larvae are in princip...

  1. Shared Ancestry of Symbionts? Sagrinae and Donaciinae (Coleoptera, Chrysomelidae) Harbor Similar Bacteria

    OpenAIRE

    Dimitra Synefiaridou; Gregor Kölsch

    2012-01-01

    When symbioses between insects and bacteria are discussed, the origin of a given association is regularly of interest. We examined the evolution of the symbiosis between reed beetles (Coleoptera, Chrysomelidae, Donaciinae) and intracellular symbionts belonging to the Enterobacteriaceae. We analyzed the partial sequence of the 16S rRNA to assess the phylogenetic relationships with bacteria we found in other beetle groups (Cerambycidae, Anobiidae, other Chrysomelidae). We discuss the ecology of...

  2. Laboratory Rearing of Laricobius nigrinus (Coleoptera: Derodontidae): A Predator of the Hemlock Woolly Adelgid (Hemiptera: Adelgidae)

    OpenAIRE

    Salom, S. M.; L. T. Kok; Lamb, A B; Jubb, C

    2012-01-01

    Coleopteran species are biological control agents of numerous invasive pests. Laricobius nigrinus (Coleoptera: Derodontidae), a predaceous, univoltine species, spends the summer aestivating but is active for the rest of the year. Laricobius nigrinus possesses many essential attributes for effective biological control of the hemlock woolly adelgid (Hemiptera: Adelgidae). The predator must be reared in large numbers for field releases. We describe some of the studies that led to the successful ...

  3. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae)

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    Andréa Roveré Franz; Neiva Knaak; Lidia Mariana Fiuza

    2011-01-01

    Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae). Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf), Zingiber officinale (root) and Mentha sp. (leaf). The efficacy of ...

  4. New species and records of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae: Macrodactylini) from Bolivia.

    Science.gov (United States)

    Arce-Pérez, Roberto; Morón, Miguel Ángel

    2014-01-01

    Two new species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae) from Bolivia are described and illustrated: M. megaphyllus new species (from Comarapa, Santa Cruz and Sehuenca, Cochabamba) and M. yunganus new species (from Mairana and Comarapa, Santa Cruz). In addition, the species Macrodactylus bolivianus Moser, M. gracilis Moser, and M. nobilis Frey are redescribed and illustrated to help facilitate identification of these species. A key to the 10 species of Macrodactylus presently known from Bolivia is provided.  PMID:25283110

  5. Wireworms’ Management: An Overview of the Existing Methods, with Particular Regards to Agriotes spp. (Coleoptera: Elateridae

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    Fanny Barsics

    2013-01-01

    Full Text Available Wireworms (Coleoptera: Elateridae are important soil dwelling pests worldwide causing yield losses in many crops. The progressive restrictions in the matter of efficient synthetic chemicals for health and environmental care brought out the need for alternative management techniques. This paper summarizes the main potential tools that have been studied up to now and that could be applied together in integrated pest management systems and suggests guidelines for future research.

  6. Lectotype designations and nomenclatural changes in Xylographus Mellié (Coleoptera, Ciidae)

    OpenAIRE

    Sandoval-Gómez,Vivian E.; Cristiano Lopes-Andrade; John Lawrence

    2014-01-01

    We designate lectotypes and propose nomenclatural changes in Xylographus Mellié (Coleoptera, Ciidae) based on type specimens deposited in the Museum of Comparative Zoology (USA), Museum für Naturkunde Berlin (Germany), the Natural History Museum (UK), Muséum d’Histoire Naturelle de la Ville de Genève (Switzerland), Muséum National d’Histoire Naturelle (France), Naturhistoriska Riksmuseet (Sweden) and Naturhistorisches Museum Wien (Austria). We desi...

  7. The Leaf-Beetles (Coleoptera, Chrysomelidae) Feed On Some Weeds In Tokat Province

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    Çam, Halit; ATAY, Turgut

    2004-01-01

    This study was carried out to determine the leaf-beetles (Coleoptera, Chrysomelidae) which causes extensive damage on weeds in the vicinity of Tokat, Turkey. Total 9 taxa including 4 species of Chrysomelinae, 1 species of Clytrinae, 1 species of Criocerinae, 2 species of Alticinae and 1 species of Cassidinae were found to be destructive on different weed species. These species were; Entomoscelis adonidis (Pall.) on Sinapis arvensis L., Gastrophysa polygoni (L.) on Polygonum convolvulus L. an...

  8. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern

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    SUGIYARTO

    2009-07-01

    Full Text Available Maryati KT, Sugiyarto. 2010. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern. Nusantara Bioscience 1: 72-77. This research aims to find out the white grub (Melolonthidae; Coleoptera variability based on the morphological characteristic and protein banding pattern found in ”salak pondoh” farm in Regencies of Sleman, Yogyakarta and Magelang, Central Java. Each area has five sampling points. Morphological analysis on white grub was conducted using descriptive method and analysis on protein banding pattern was conducted using qualitative analysis based on the presence or absent of band pattern on the gel, and qualitatively based on the relative mobility value (Rf of protein. The result indicated that the white grub in Sleman and Magelang, based on morphology characteristic is only one species, namely Holothricia sp. Based on the protein banding pattern, the white grub sample have differences of protein band number and protein molecular weight. Key words: Salacca zalacca, white grub, morphology, protein banding pattern.Abstrak. Maryati KT, Sugiyarto. 2010. Karakterisasi lundi putih (Melolonthidae: Coleoptera pada pertanaman salak berdasarkan ciri morfologi dan pola pita protein. Nusantara Bioscience 1: 72-77. Penelitian ini bertujuan untuk mengetahui keanekaragaman lundi putih (Melolonthidae; Coleoptera berdasarkan ciri morfologi dan pola pita protein yang ditemukan di lahan pertanaman salak pondoh di Kabupaten Sleman, Yogyakarta dan Kabupaten Magelang, Jawa Tengah. Pada masing-masing wilayah diambil lima titik sampling. Analisis morfologi lundi putih digunakan metode deskriptif, dan analisis pola pita protein digunakan analisis kualitatif berdasarkan muncul tidaknya pola pita pada gel, dan secara kuantitatif berdasarkan nilai mobilitas relatif protein (RF. Hasil penelitian menunjukkan bahwa sampel lundi putih di Kabupaten Sleman dan Magelang, berdasar karakter

  9. A new genus and four new species of false click beetles (Coleoptera: Eucnemidae) from Southeast Asia

    OpenAIRE

    Otto, Robert L.

    2016-01-01

    A new genus and four new species of false click beetle (Coleoptera: Eucnemidae) are described. Bermilloides new genus is described from Bornean Malaysia. New species are: Calyptocerus iridis new species (Philippines), Bermilloides lumawigi new species (Malaysia), Spinifornax elongatus new species (Malaysia) and Ceratus antennatus new species (Thailand). Dorsal and ventral habitus, as possible, for each species are illustrated. Male aedeagi are illustrated for Calyptocerus iridis and Ceratu...

  10. Life History of the Tamarind Weevil, Sitophilus linearis (Herbst) (Coleoptera: Curculionidae), on Tamarind Seed

    OpenAIRE

    James Adebayo Ojo; Adebayo Amos Omoloye

    2015-01-01

    The tamarind weevil, Sitophilus linearis Herbst (Coleoptera: Curculionidae), is an important pest of tamarind and other Caesalpinioideae. Investigating its life history is important in the implementation of management strategy. Its life history was monitored daily to understand its developmental biology on tamarind seed following standard procedures under laboratory conditions of 24–30°C temperature, 60–70% relative humidity, and 12L : 12D photoperiod. The egg incubation period lasted 3.17 ± ...

  11. Studies on the Dung-inhabiting Beetles (Insecta: Coleoptera Community of Western Anatolia, Turkey

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    Sinan Anlaş

    2011-12-01

    Full Text Available In Bozdağlar Mountain of western Turkey, the diversity and composition of the dung-inhabiting beetles in two locations situated in different altitudes (600 m and 900 m in 2004 and 2006 assemblages were sampled. A total of 5.709 individuals from 88 species belonging to the families Scarabaeidae, Aphodiidae, Geotrupidae, Carabidae, Hydrophilidae, Histeridae and Ptilidae of the order Coleoptera are recorded.

  12. Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil

    OpenAIRE

    Andreia Mauruto Chernaki-Leffer; Daniel Ricardo Sosa-Gómez; Almeida, Lúcia M.; Ivani de Oliveira Negrão Lopes

    2011-01-01

    Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer), is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvo...

  13. Novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae da Bolívia

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    Ubirajara R. Martins

    2008-01-01

    Full Text Available Novas espécies descritas da Bolívia, Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.New species of Onciderini (Coleoptera, Cerambycidae, Lamiinae from Bolivia. New species described from Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.

  14. Penggunaan Beberapa Serbuk Rimpang Terhadap Mortalitas Hama Callosobruchus chinensis L. (Coleoptera; Bruchidae) Pada Kacang Hijau

    OpenAIRE

    Hayati, Anni

    2012-01-01

    Anni Hayati, "Use of Some Powdered Rhizome Mortality Against Pests Callosobruchus chinensis L. (Coleoptera: Bruchidae) On the Green Bean ". It was under supervised by Yuswani Pangestiningsih and Fatimah Zahara. C.chinensis L. are pests on green beans in storage which causes considerable yield loss, one of effective control is the use of botanical insecticides. Use of some powdered rhizome on mortality C.chinensis L. in a green bean plant pests carried in the laboratory of t...

  15. Ernährungsphysiologische Aspekte xylophager Bockkäferlarven (Coleoptera: Cerambycidae)

    OpenAIRE

    Grünwald, Sebastian

    2009-01-01

    Mittels Fluoreszenz-in-situ-Hybridisierung und PCR-basierter rRNA-Sequenzierung wurde die Darm- und Myzetomflora xylophager Insektenlarven (Coleoptera: Cerambycidae) untersucht. Die Myzetome enthalten hefeartige Symbionten in Form von Candida rhagii und Candida shehatae. Das Darmlumen der Tiere beherbergt eine hoch variable Gemeinschaft von Bakterien und Pilzen, die zum Teil der Nahrung entstammt. Die Larven des Hausbockkäfers nehmen eine Sonderstellung ein: Ihnen fehlen sowohl Myzetome als a...

  16. Defensive Glands of the Darkling Beetle Mesomorphus villiger Blanchard (Coleoptera: Tenebrionidae)

    OpenAIRE

    Seena, C. M.; Sabu K. Thomas

    2013-01-01

    Massive home invasion by the darkling beetle Mesomorphus villiger Blanchard 1853 (Coleoptera: Tenebrionidae) during monsoon season make it a nuisance pest in many regions of south India. Morphology of defensive glands and mode of release and dispersal of the defensive secretion were analysed. Defensive glands were separated from the abdominal sternites by cutting along the posterior margin of the seventh sternite. Glands are evaginations of intersegmental membrane between the seventh and eigh...

  17. DIVERSITY OF CARABIDS (COLEOPTERA) ASSOCIATED WITH THE LOWER LURIN RIVER, LIMA, PERU

    OpenAIRE

    ARMANDO VÉLEZ-AZAÑERO; Alfonso Lizárraga-Travaglini

    2013-01-01

    Quarterly samples of beetles (Coleoptera: Carabidae) were taken associated with the lower basin of the Lurín River (Lima-Peru) during the period August 2009 - February 2011, in six sampling points between 5 and 51 masl. Pitfall traps were used and obtained a total of 59 specimens distributed among three tribes, three genera, and four morphospecies. We report the presence of Megacephala, Scarites genus, and Pterostichus with the latter being the predominant genus.

  18. New national and state records of Neotropical Staphylinidae (Insecta: Coleoptera).

    Science.gov (United States)

    Asiain, Julieta; Márquez, Juan; Irmler, Urlich

    2015-01-01

    Eighteen new national records of nine species of Osoriinae (Staphylinidae) are added for 10 Neotropical countries. Additionally, 17 species of three subfamilies are first recorded from ten States of México. The distributional patterns of the studied species are commented and the congruence with species of different families of Coleoptera and Odonata previously analyzed is discussed. Finally, we conclude that some of these patterns can be proposed as hypothesis of primary biogeographic homology. PMID:26249885

  19. Incorporating a Sorghum Habitat for Enhancing Lady Beetles (Coleoptera: Coccinellidae) in Cotton

    OpenAIRE

    P. G. Tillman; Cottrell, T. E.

    2012-01-01

    Lady beetles (Coleoptera: Coccinellidae) prey on insect pests in cotton. The objective of this 2 yr on-farm study was to document the impact of a grain sorghum trap crop on the density of Coccinellidae on nearby cotton. Scymnus spp., Coccinella septempunctata (L.), Hippodamia convergens Guérin-Méneville, Harmonia axyridis (Pallas), Coleomegilla maculata (De Geer), Cycloneda munda (Say), and Olla v-nigrum (Mulsant) were found in sorghum over both years. Lady beetle compositions in sorghum and ...

  20. A checklist of the genus Blosyrus Schoenherr (Coleoptera: Curculionidae: Entiminae of the world

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    G. Mahendiran

    2013-08-01

    Full Text Available An annotated checklist of Blosyrus Schoenherr (Coleoptera: Curculionidae: Entiminae comprising 101 species with their updated nomenclature, synonyms and distribution is given. The distribution pattern indicates that the genus is diversified mostly in the Sub-Saharan Africa and Asia regions. Out of 101 species, 92 occur in Sub-Saharan Africa. From India, eight species were recorded. In India, the distribution is mainly in West Bengal, Assam, Maharashtra, Andhra Pradesh, Karnataka and Tamil Nadu.

  1. Biological activities of Allium sativum essential oil against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae)

    OpenAIRE

    Chaubey Mukesh Kumar

    2014-01-01

    Essential oil from Allium sativum was isolated and investigated for its repellent, insecticidal, ovipositional and egg hatching inhibition activities against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae). A. sativum essential oil repelled bruchid adults at a very low concentration in choice oviposition assay. A. sativum essential oil caused both fumigant and contact toxicity in C. chinensis adults in a concentration dependent manner. Oviposition potency of C. chinensis adults...

  2. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae)

    OpenAIRE

    M.M. Kiradoo; Meera Srivastava

    2011-01-01

    The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae) is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insect...

  3. Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)

    Science.gov (United States)

    Nenadić, Marija; Soković, Marina; Glamočlija, Jasmina; Ćirić, Ana; Perić-Mataruga, Vesna; Ilijin, Larisa; Tešević, Vele; Vujisić, Ljubodrag; Todosijević, Marina; Vesović, Nikola; Ćurčić, Srećko

    2016-04-01

    The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens—eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var . cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var . cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.

  4. Especies mexicanas de Curculionidae (Insecta: Coleoptera asociadas con agaves (Asparagaceae: Agavoideae Mexican species of Curculionidae (Insecta: Coleoptera associated to agaves (Asparagaceae: Agavoideae

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    Alberto Romo

    2012-12-01

    Full Text Available Se estudiaron las especies de picudos o gorgojos (Coleoptera: Curculionidae asociadas con agaves (Asparagaceae: Agavoideae en México. Se registraron 5 especies asociadas con especies de Agave, Furcraea, Hesperoyucca, Polianthes y Yucca; de éstas, 4 pertenecen a la subfamilia Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis y Cactophagus spinolae y 1 a la Baridinae (Peltophorus polymitus. Se presentan diagnosis, ilustraciones y una clave para la identificación de las 5 especies de curculiónidos asociados con agaves. Se establecen las siguientes sinonimias: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; y Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891 y Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.Weevils (Coleoptera: Curculionidae associated with agaves (Asparagaceae: Agavoideae from Mexico are analyzed. Five species were recorded associated with species of Agave, Furcraea, Hesperoyucca, Polianthes, and Yucca. Four of these species belong to the subfamily Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis, and Cactophagus spinolae and 1 belongs to the subfamily Baridinae (Peltophorus polymitus. Diagnoses, illustrations and a key are presented for identifying the 5 species of weevils found on agaves. The following synonymies are established: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; and Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891, and Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.

  5. Behavioral responses of plum curculio (Coleoptera: Curculionidae) to different enantiomer concentrations and blends of the synthetic aggregation pheromone grandisoic acid

    Science.gov (United States)

    Host plant odors are important for insect location of food and mates. Synergy between host plant odors and aggregation pheromones occurs in many Curculionidae species. The plum curculio Conotrachelus nenuphar Herbst (Coleoptera: Curculionidae) is a major pest of pome and stone fruit. Males produce t...

  6. From forest to plantation? Obscure papers reveal alternate host plants for the coffee berry borer, Hypothenemus hampei (Coleoptera: Curculionidae)

    Science.gov (United States)

    The coffee berry borer, Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae), is the most devastating insect pest of coffee throughout the world. The insect is endemic to Africa but can now be found throughout nearly all coffee producing countries. One area of the basic biology of the insec...

  7. First record of the agave snout weevil, Scyphophorus acupunctatusGyllenhal (Coleoptera: Curculionidae: Dryophthorinae), in Puerto Rico

    OpenAIRE

    Setliff, Gregory P.; Anderson, Jesse A.

    2011-01-01

    The agave snout weevil, Scyphophorus acupunctatus Gyllenhal (Coleoptera: Curculionidae: Dryophthorinae), is reported from Puerto Rico for the first time. It was collected on feral sisal, Agave sisalana Perrine (Agavaceae), in the Guánica Dry Forest Reserve in the southwestern part of the island.

  8. Redescription of the Hispaniolan ladybird genus Bura Mulsant (Coleoptera: Coccinellidae) and justification for its transfer from Coccidulinae to Sticholotidinae

    Science.gov (United States)

    In the current work, we discuss the features of Bura (Coleoptera: Coccinellidae) that justify its transfer from Coccidulinae to Sticholotidinae, speculate on circumstances that led to its prior misclassification, and highlight current problems in the delineation of the afforementioned lady beetle su...

  9. A new genus for Drepanocanthoides larreae (Horn, 1887) and description of a new congeneric Mexican species (Coleoptera: Scarabaeidae: Aphodiinae)

    OpenAIRE

    Marco DELLACASA; Dellacasa, Giovanni; Gordon, Robert D.

    2016-01-01

    Drepanocanthoides larreae (Horn, 1887) (Coleoptera: Scarabaeidae: Aphodiinae) is transferred to the new genus Hornosus and the new species Hornosus turnbowi from Mexico (Nuevo León) is described. A complete set of fi gures is supplied for the taxa dealt with herein.

  10. Saprovisca sarangay, new species, a second species in an unusual Philippine genus of Eupariini (Coleoptera: Scarabaeidae: Aphodiinae)

    OpenAIRE

    Skelley, Paul E.

    2016-01-01

    A new species of Saprovisca Stebnicka (Coleoptera: Scarabaeidae) is described from the Philippines. This new species possesses sexual dimorphism in the form of clypeal horns in males which also show major and minor allometry, both are conditions rare in the Aphodiinae.

  11. Agrilus rubensteini, a new species from the Philippines related to the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae)

    Science.gov (United States)

    A new species from the Philippines closely related to the emerald ash borer, Agrilus planipennis Fairmaire, 1888 (Coleoptera: Buprestidae) is described: Agrilus rubensteini Chamorro & Jendek, new species. This is the first species in the A. cyaneoniger species-group recorded for the Philippines. Agr...

  12. Upper lethal temperature limits of the common furniture beetle Anobium punctatum (Coleoptera: Anobiidae)

    DEFF Research Database (Denmark)

    Hansen, Lise Stengård; Jensen, Karl-Martin Vagn

    The susceptibility of the egg, larval and adult stages of Anobium punctatum De Geer (Coleoptera: Anobiidae) to heat (46-54°C, 25-30% RH) was investigated. The larval stage was found to be most tolerant to heat. Very short exposure (5 min) of the larvae to temperatures of 52°C and above led to 100...

  13. Records of unsuccessful attack by Anoplophora glabripennis (Coleoptera: Cerambycidae) on broadleaf trees of questionable suitability in Ontario

    Science.gov (United States)

    Discovery of the non-native Anoplophora glabripennis Motschulsky (Coleoptera: Cerambycidae) in Ontario, Canada, in 2003 led to the implementation of an eradication program. The plan consisted of removing all infested trees and all trees within 400 m of an infested tree belonging to a genus consider...

  14. Seasonal flight activity and distribution of metallic woodboring beetles (Coleoptera: Buprestidae) collected in North Carolina and Tennessee

    Science.gov (United States)

    Metallic wood boring insects (Coleoptera: Buprestidae) species are responsible for high levels of host plant injury to deciduous shade and flowering trees in commercial nurseries, urban forests, and managed landscapes. Ornamental plant producers in the southeastern U.S. have ranked borers, includin...

  15. Resistance in Cultivated Sunflower Germplasm to the Red Sunflower Seed Weevil (Coleoptera: Curculionidae) in the Northern Great Plains

    Science.gov (United States)

    A 6-year field study evaluated 52 sunflower, Helianthus annuus L., accessions, 20 breeding lines, and 9 interspecific crosses for resistance to infestation by naturally occurring populations of the red sunflower seed weevil, Smicronyx fulvus LeConte (Coleoptera: Curculionidae). Germplasm with potent...

  16. Introduction and Recovery of Delphastus catalinae (Coleoptera: Coccinellidae) as a predator of Bemisia tabaci (Hemiptera: Aleyrodidae) in Egypt

    Science.gov (United States)

    Bemisia tabaci (Gennadius) (Hemiptera: Alyerodidae) is an important pest of many crops on a global scale. The use of biological control organisms such as coccinelid predators can help manage this pest. Delphastus catalinae (Horn) (Coleoptera: Coccinelidae) is an obligate predator of whiteflies, in...

  17. Quantitative relationship between potato tuber damage and counts of Pacific Coast wireworm (Coleoptera: Elateridae) in baits: seasonal effects.

    Science.gov (United States)

    Plots were baited with rolled oats in spring to assess the relationship between counts of Pacific coast wireworm, Limonius canus (Coleoptera: Elateridae) and damage to potato tubers. Baiting was done at 7 intervals beginning before planting of potatoes and ending following germination. Injury (per...

  18. Self-selection of two diet components by Tennebrio molitor (Coleoptera: Tenebrionidae) larvae and its impact on fitness

    Science.gov (United States)

    We studied the ability of Tenebrio molitor L. (Coleoptera: Tenebrionidae) to self-select optimal ratios of two dietary components to approach nutritional balance and maximum fitness. Life table analysis was used to determine the fitness of T. molitor developing in diet mixtures comprised of four dif...

  19. Novos táxons em Acanthocinini sul-americanos (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Marcela L. Monné

    2012-09-01

    Full Text Available Novos táxons em Acanthocinini sul-americanos (Coleoptera, Cerambycidae. São descritos e figurados os novos táxons: Trichonyssodrys nessimiani sp. nov. do Brasil (Bahia a Santa Catarina, Amniscites tavakiliani sp. nov. da Guiana Francesa and A. amboroensis sp. nov. da Bolívia (Cochabamba e Santa Cruz; Trichonius inusitatus sp. nov. e Lophopoenopsis itatiaiensis sp. nov. do Brasil (Rio de Janeiro e Luteolepturges gen. nov., espécie tipo L. galbus sp. nov., do Brasil (Amazonas, Rondônia, Peru e Equador.

  20. Nothoprodontia, um novo gênero de Trachyderini (Coleoptera, Cerambycidae, Cerambycinae

    Directory of Open Access Journals (Sweden)

    Miguel A. Monné

    2004-06-01

    Full Text Available Nothoprodontia gen. nov. e sua espécie-tipo, N. boliviana sp. nov., são descritos da Bolívia (Cochabamba. O novo gênero é comparado com Prodontia Audinet-Serville, 1834, Eriphus Audinet-Serville, 1834, and Athetesis Bates, 1870.Nothoprodontia, a new genus of Trachyderini (Coleoptera, Cerambycidae, Cerambycinae. Nothoprodontia gen. nov. and its type-species, N. boliviana sp. nov., are described from Bolívia (Cochabamba. Comparative notes with Prodontia Audinet-Serville, 1834, Eriphus Audinet-Serville, 1834, and Athetesis Bates, 1870 are also given.

  1. Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico

    Directory of Open Access Journals (Sweden)

    Sonia Maria Noemberg Lazzari

    2006-06-01

    Full Text Available Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico. O objetivo desta pesquisa foi avaliar o efeito do resfriamento artificial de grãos de arroz para o controle de coleópteros-praga. O ar frio foi insuflado pelo sistema de aeração em um silo metálico com arroz-em-casca. A avaliação do tratamento foi feita quinzenalmente usando armadilhas caladores. As espécies de Coleoptera capturadas foram: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16,5% e Sitophilus spp. (0,5%. Aos 28 dias, a temperatura média da massa de grãos era de 15ºC, e o número médio de insetos havia diminuído 76,8%. A aplicação de ar frio manteve as populações sob controle por aproximadamente 60 dias. Os resultados do monitoramento dos insetos e da temperatura indicaram que um novo ciclo de ar frio deveria ser aplicado nesse período para manter as populações sob controle. Também o manejo adequado da massa de grãos faz-se necessário para garantir resultados satisfatórios do resfriamento artificial.Artificial chilling to control Coleoptera in paddy rice stored in metallic silo. The objective of this research was to evaluate the effect of artificial chilling for the control of coleopterans in stored paddy rice. The cold air was insufflated through the aeration system of a metallic silo with paddy rice. Evaluation of insect number was made every 15 days using probe traps. The species of Coleoptera captured were: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16.5% and Sitophilus spp. (0.5%. By the 28th day the average temperature of the grain mass was 15ºC, and the mean number of insects decreased 76.8%. The cold air application kept the insect populations under control for approximately 60 days. The results of temperature and insect monitoring indicated that a new cycle of cold air should be applied by that time to keep the populations under

  2. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-09-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  3. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-06-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  4. Invertebrate fauna (Coleoptera, Collembola, Diplopoda, Isopoda collected in the karst areas of the Aninei - Locvei Mountains

    Directory of Open Access Journals (Sweden)

    Andrei Giurginca

    2011-08-01

    Full Text Available The authors identified 132 species of invertebrates (14 Oniscidea, 25 Diplopoda,31 Collembola and 62 Coleoptera recently sampled (2001–2006 from the soil and subterranean (MSS and caves environments from the Banat Mountains. Some new,rare and endemic species are discussed. The seasonal changes of the species diversity in the superficial subterranean environments at 0.5 to 1 m in depth are for the first time presented for the Reşiţa – Moldova Nouă synclinorium. The characteristic and preferential species for the mesovoid shallow substratum (MSS, belonging to the analyzed taxa, are identified.

  5. A review of the natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries.

    Science.gov (United States)

    Di Iorio, Osvaldo

    2014-01-01

    A compilation of the known natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries is provided. Food items of adult Cetoniinae include pollen and/or nectar (flower visitors), sap and/or slime flux, ripened fruits on plants, green tissues and leaves, and honey. Of the 36 species of Cetoniinae from Argentina, food items are known only for 11 species (30.5%). Attraction to light and bait-traps, adult activity periods, vertebrate predators, and the occurrence in bird nests are presented and discussed. Other insects that share the same food sources and bait-traps with Cetoniinae are mentioned. PMID:24869870

  6. Description of the third instar larvae of five species of Cyclocephala (Coleoptera, Melolonthidae, Dynastinae from Mexico

    Directory of Open Access Journals (Sweden)

    Miguel A. Morón

    2014-09-01

    Full Text Available Description of the third instar larvae of five species of Cyclocephala (Coleoptera, Melolonthidae, Dynastinae from Mexico. Larvae of four species of Cyclocephala are described for the first time based on specimens collected in Mexican localities: C. barrerai Martínez, 1969 from Puebla, C. sinaloae Howden & Endrödi, 1966 from Sinaloa, C. fasciolata Bates, 1888 from Veracruz, and C. jalapensis Casey, 1915 from Hidalgo. Larva of C. lunulata Burmeister, 1847, is redescribed based on specimens from the Mexican states of Morelos, Puebla, and Veracruz. Diagnostic structures are illustrated and the differences and similarities of each species with other previously described larvae of the genus are commented.

  7. Espécies de Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini ocorrentes na Bolívia

    Directory of Open Access Journals (Sweden)

    Maria Helena M. Galileo

    2013-03-01

    Full Text Available Species of Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini occurring in Bolivia. The genus Gorybia (Cerambycinae, Piezocerini consists of 45 described species with seven species recorded from Bolivia. Nine new species are described herein from Bolivia: G. abnormalis sp. nov.; G. alveolata sp. nov.; G. asyka sp. nov.; G. florida sp. nov.; G. inarmata sp. nov.; G. longithorax sp. nov.; G. guenda sp. nov.; G. tuberosa sp. nov. and G. wappesi sp. nov. A key to the species now known to occur in Bolivia is included.

  8. On the family- and genus-series nomina in Gyrinidae Latreille, 1810 (Coleoptera, Adephaga).

    Science.gov (United States)

    Gustafson, Grey T; Miller, Kelly B

    2013-01-01

    All available genus- and family- group nomina for the Gyrinidae (Coleoptera: Adephaga) are listed along with original citation, original and current status, type nominal taxon with method of designation, and known synonymies and incorrect subsequent spellings. The nomina included follow the most current classification. Discussion is provided clarifying numerous nomenclatural problems with original spellings, correct authorship and type designation. Dineutini Ochs, 1926 syn. nov. is found to be a junior homonym of Dineutini Desmarest, 1851, and Enhydrini Régimbart, 1882 syn. nov. and its justified emendation Enhydrusini (Anonymous 2012) are here synonymized with Dineutini Desmarest, 1851. PMID:25277555

  9. Gross anatomy of central nervous system in firefly, Pteroptyx tener (Coleoptera: Lampyridae)

    Science.gov (United States)

    Hudawiyah, Nur; Wahida, O. Nurul; Norela, S.

    2015-09-01

    This paper describes for the first time the organization and fine structure of the central nervous system (CNS) in the fireflies, Pteroptyx tener (Coleoptera: Lampyridae). The morphology of the CNS was examined by using Carl Zeiss AxioScope A1 photomicroscope with iSolution Lite software. Some specific structural features such as the localization of protocerebrum, deutocerebrum and tritocerebrum in the brain region were analyzed. Other than that, the nerve cord and its peripheral structure were also analyzed. This study suggests that, there is a very obvious difference between male and female central nervous system which illustrates that they may differ in function in controlling physiological and behavioral activities.

  10. Soil and saproxylic species (Coleoptera, Collembola, Araneae in primeval forests from the northern part of South-Easthern Carpathians

    Directory of Open Access Journals (Sweden)

    Eugen Nitu

    2009-11-01

    Full Text Available In 2006-2007 we carried out faunal investigations in the vernal, estival and autumnal seasons in the scientific reserve "Codrul Secular Giumalãu" using quantitative sampling methods. We identified 189 species of Coleoptera, 70 of Collembola and 20 of Araneae. Of these, 11 phytophagous, 18 myceto/xylo-mycetophagous,9 mixophagous, 18 xylo- and cambio-xylemophagous, 38 saproxylophagous,125 (55 Coleoptera, 70 Collembola detritivorous (sapro-, copro- andnecrophagous, 60 (40 Coleoptera, 20 Aranea predators/parasitoids. Hymenaphorura polonica Pomorski, 1990 (Collembola, and Leiodes rhaeticus Erichson, 1845 (Coleoptera, Leiodidae, are recorded for the first time in the Romanian fauna. The rare species and characteristic species for the old primeval spruce forests are analysed for each studied taxonomic group. The species richness and faunal diversity from the Giumalãu primeval spruce forest are compared with those of other very well preserved forests from the Carpathians scientific reserves (Codrul Secular Slãtioara,Pietrosul Rodnei. The species abundances were used to compute the similarity indexes between the sampled sectors of forest and to perform Cluster Analysis. We observed that the dead wood in the 2nd-6th phases of decomposition has a great influence not only on the saproxylic species but also on the soil fauna like ground beetles(Carabidae that use the logs as ecologic microrefuges (winter refugees or diurnal refugees. The structure of the soil fauna is influenced by wood extraction from the forest ecosystem or by natural perturbations, this consisting in the appearance of opportunistic species as Orchesella pontica (Collembola and in decreasing ofspecies richness of Carabidae (Coleoptera.

  11. DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR Arzu ÜNAL, Ahmet ÇABUK, Nazif KOLONKAYA

    Directory of Open Access Journals (Sweden)

    Nazif KOLANKAYA

    2011-08-01

    Full Text Available Detoxification of a chlorinated phenolic compound, 2,4,6- trichlorophenol through treatment with laccase enzyme produced by a white rot fungus, Trametes versicolor was investigated. Enzymaticdechlorination experiments by using free and immobilized laccase have been performed in a lab scale bioreactor. Chlorine ion and dissolved oxygen electrodes mounted to the bioreactor were used continuouslyto detect the profiles of chlorine ions and oxygen consumption, respectively, in reaction medium. The maximum dechlorination activity of laccase for free and immobilized form was determined as 160 μM of substrate concentration at pH 5.0, 25 °C, and 30 min of incubation time. Also, GC/MS analyses of enzymatic degradation products indicated that chlorine removal was a result of degradation of 2,4,6- trichlorophenol by the laccase under the determined optimum conditions.

  12. The hydraulic mechanism in the hind wing veins of Cybister japonicus Sharp (order: Coleoptera).

    Science.gov (United States)

    Sun, Jiyu; Wu, Wei; Ling, Mingze; Bhushan, Bharat; Tong, Jin

    2016-01-01

    The diving beetles (Dytiscidae, Coleoptera) are families of water beetles. When they see light, they fly to the light source directly from the water. Their hind wings are thin and fragile under the protection of their elytra (forewings). When the beetle is at rest the hind wings are folded over the abdomen of the beetle and when in flight they unfold to provide the necessary aerodynamic forces. In this paper, the unfolding process of the hind wing of Cybister japonicus Sharp (order: Coleoptera) was investigated. The motion characteristics of the blood in the veins of the structure system show that the veins have microfluidic control over the hydraulic mechanism of the unfolding process. A model is established, and the hind wing extending process is simulated. The blood flow and pressure changes are discussed. The driving mechanism for hydraulic control of the folding and unfolding actions of beetle hind wings is put forward. This can assist the design of new deployable micro air vehicles and bioinspired deployable systems. PMID:27547607

  13. Dung beetles (Coleoptera, Scarabaeinae attracted to sheep dung in exotic pastures

    Directory of Open Access Journals (Sweden)

    César Murilo de Albuquerque Correa

    2013-03-01

    Full Text Available Dung beetles (Coleoptera, Scarabaeinae attracted to sheep dung in exotic pastures. In this study we provide data on the abundance and richness of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae attracted to sheep dung in exotic pastures (Brachiaria spp.. In four areas of exotic pasture pitfall traps were installed and baited with fresh sheep dung for sampling of dung beetles. A total of 2,290 individuals were captured belonging to 16 species, 10 genera and five tribes of Scarabaeinae beetles. Trichillum externepunctatum Preudhomme de Borre, 1886 and Dichotomius bos (Blanchard, 1843 were dominant. The guild of dwellers was the most abundant in pastures. We demonstrate that dung beetles are attracted to sheep dung. Since the production of both cattle and sheep in the same area is common in tropical pasturelands, results obtained here highlight the need to investigate the actual role of dung sharing (cattle dung + sheep dung by dung beetles. It is also suggested that experiments be performed for evaluation of the ecological functions performed by dung beetles using sheep dung.

  14. Ophiostoma species (Ascomycetes: Ophiostomatales) associated with bark beetles (Coleoptera: Scolytinae) colonizing Pinus radiata in northern Spain.

    Science.gov (United States)

    Romón, Pedro; Zhou, XuDong; Iturrondobeitia, Juan Carlos; Wingfield, Michael J; Goldarazena, Arturo

    2007-06-01

    Bark beetles (Coleoptera: Scolytinae) are known to be associated with fungi, especially species of Ophiostoma sensu lato and Ceratocystis. However, very little is known about these fungi in Spain. In this study, we examined the fungi associated with 13 bark beetle species and one weevil (Coleoptera: Entiminae) infesting Pinus radiata in the Basque Country of northern Spain. This study included an examination of 1323 bark beetles or their galleries in P. radiata. Isolations yielded a total of 920 cultures, which included 16 species of Ophiostoma sensu lato or their asexual states. These 16 species included 69 associations between fungi and bark beetles and weevils that have not previously been recorded. The most commonly encountered fungal associates of the bark beetles were Ophiostoma ips, Leptographium guttulatum, Ophiostoma stenoceras, and Ophiostoma piceae. In most cases, the niche of colonization had a significant effect on the abundance and composition of colonizing fungi. This confirms that resource overlap between species is reduced by partial spatial segregation. Interaction between niche and time seldom had a significant effect, which suggests that spatial colonization patterns are rarely flexible throughout timber degradation. The differences in common associates among the bark beetle species could be linked to the different niches that these beetles occupy. PMID:17668036

  15. ESCARABAJOS TIGRE (COLEOPTERA: CICINDELIDAE DEL MUSEO ENTOMOLÓGICO FRANCISCO LUÍS GALLEGO: NUEVOS REGISTROS PARA DEPARTAMENTOS DE COLOMBIA TIGER BEETLES (COLEOPTERA: CICINDELIDAE AT THE ENTOMOLOGICAL MUSEUM FRANCISCO LUÍS GALLEGO: NEW RECORDS

    Directory of Open Access Journals (Sweden)

    Manuel Alejandro Ramírez Mora

    2008-06-01

    Full Text Available Se registran por primera vez las especies de escarabajos tigre (Coleoptera: Cicindelidae presentes en el Museo Entomológico Francisco Luis Gallego (MEFLG. Se identificaron 167 especimenes distribuidos en ocho géneros y 27 especies, se reportan 24 nuevos registros para diferentes departamentos de Colombia. Se señalan aspectos importantes de la taxonomía y sistemática del grupo. Además, se presentan comentarios biológicos y de distribución de las especies.Tiger beetles (Coleoptera: Cicindelidae species at the Museo Entomológico Francisco Luis Gallego (MEFLG are registered by first time. 167 specimens in 8 genus and 27 species were identified, 24 new records for different Colombian states are reported. Some important aspects of the group’s taxonomy and systematic are pointed. Additionally, species’ biological and distributional comments are presented.

  16. Especies de Megacerus (Coleoptera: Bruchidae y sus plantas hospederas en Yucatán Megacerus species (Coleoptera: Bruchidae and their host plants in Yucatán

    Directory of Open Access Journals (Sweden)

    Enrique Reyes

    2009-12-01

    Full Text Available Se registra la incidencia de Megacerus (Coleoptera: Bruchidae en semillas de 7 especies de Convolvulaceae de 6 sitios al norte de Yucatán. Las semillas de Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia y M. cissoides recolectadas en etapa de dispersión fueron colocadas en sobres limpios de papel y puestas en condiciones de laboratorio hasta la emergencia de los brúquidos. Se obtuvieron 1111 coleópteros pertenecientes a 5 especies: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, y M. (Serratibruchus cubiciformis. Las semillas de I. crinicalyx, M. aegyptia y M. cissoides fueron infestadas principalmente por M. (M. porosus, las de I. hederifolia e I. triloba por M. (M. cubiculus y las de I. nil por M. (M. tricolor. Todos los individuos de Megacerus (M. sp., fueron encontrados en semillas de J. penthanta. En el nivel génerico de hospedero se encontró que las semillas de Ipomoea fueron infestadas principalmente por M. (M. cubiculus y las de Merremia por M. (M. porosus. Estos resultados amplían el rango de plantas hospederas registrado para las especies de Megacerus y resaltan la estrecha asociación que existe entre un linaje de depredadores de semillas Bruchidae y plantas de la familia Convolvulaceae.Seed-attacking by Megacerus (Coleoptera: Bruchidae is reported for 7 Convolvulaceae species from 6 sites at the North of Yucatan. Seeds of Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia and M. cissoides were collected and placed in clean envelopes under laboratory conditions to collect all bruchids emerged from seeds. A total of 1111 coleopterans were hatched from seeds, and 5 taxa were identify: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, and M. (Serratibruchus cubiciformis. Seeds of I. crinicalyx, M. aegyptia and M. cissoides were attacked mainly by M. (M. porosus; I

  17. Longhorned Beetles Collection of the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey (Coleoptera,Cerambycidae)

    OpenAIRE

    ÖZDİKMEN, Hüseyin; ŞAHİN, Özlem

    2010-01-01

    In the present paper, specimens of Cerambycidae were examined in the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey. As a result of identification of these specimens, thirty-eight species and two subspecies belonging to twenty-eight genera of five subfamilies were determined. With this paper, new faunistic data and some zoogeographical evaluations were presented on longhorned beetles fauna (Coleoptera, Cerambycidae) of Turkey. The faunistic data in the pr...

  18. Synergistic effects of chlorpyrifos with piperonyl butoxide (pbo) against the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

    OpenAIRE

    Akter Mst Yeasmin; Talukdar Muhammad Waliullah; ASM Shafiqur Rahman

    2014-01-01

    Objective: To investigate the co-toxicity and co-efficient activity of Chlorpyrifos (Dursban 20EC), an organophosphate and Piperonyl butoxide (PBO) against the lesser meal worm Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) (A. diaperinus). Methods: The repellent activity was carried out by the residual film assay technique. Statistically the dose mortality relationship was expressed as a median lethal dose (LD50) by the probit analysis. The regression lines and isoboles were ...

  19. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

    OpenAIRE

    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineens...

  20. Natural history of Belonuchus Nordmann spp. and allies (Coleoptera: Staphylinidae) in Heliconia L. (Zingiberales: Heliconiaceae) flower bracts

    OpenAIRE

    Frank, J. Howard; Barrera, Roberto

    2010-01-01

    Adults, and in some species larvae, of several members of Belonuchus Nordmann (Coleoptera: Staphylinidae: Staphylininae) and a few related genera seem to be to various degrees consistently associated with flower bracts of the genus Heliconia (Zingiberales: Heliconiaceae). They are predators and eat various dipterous and lepidopterous larvae in that habitat. Adults of at least Belonuchus cephalotes (Sharp) and Odontolinus fasciatus Sharp are able to immerse completely in water to capture larva...

  1. Uji Efektifitas Beberapa Insektisida Nabati Terhadap Mortalitas Hama Sitophilus zeamais Motsch. (Coleoptera; Curculionidae) Pada Benih Jagung (Zea mays)

    OpenAIRE

    Subhan, Fadillah

    2011-01-01

    Fadillah Subhan, "Test Effectiveness of Some Insecticides Against Plant Pests Sitophilus zeamais Motsch Mortality. (Coleoptera: Curculionidae) In the Seed of Corn (Zea mays). "It was under supervised by Yuswani Pangestiningsih and Fatimah Zahara. S. zeamais is a pest of corn in storage that causes substantial yield losses, one of which effective control is the use of botanical insecticides. Testing the effectiveness of some botanical insecticides on mortality of S. zeamais on maize seed plant...

  2. A review of Elocomosta Hansen with a description of a new species with reduced eyes from China (Coleoptera, Hydrophilidae, Sphaeridiinae)

    Science.gov (United States)

    Lin, Renchao; Jia, Fenglong; Fikáček, Martin

    2016-01-01

    Abstract A new species of the genus Elocomosta Hansen, 1989 (Coleoptera: Hydrophilidae: Sphaeridiinae: Coelostomatini), Elocomosta lilizheni sp. n., is described from Guangxi Province, China. It is compared in detail with the only other known species of the genus, Elocomosta nigra Hansen, 1989 from Borneo, and the genus is diagnosed from the remaining coelostomatine genera. The new species is unusual among Hydrophilidae by having extremely reduced eyes. PMID:27551232

  3. Diversity and phylogenetic analysis of endosymbiotic bacteria of the date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae)

    OpenAIRE

    El-Sayed, Wael S.; Ibrahim, Reda A

    2015-01-01

    Background The date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae) is one of the major pests of palms. In Saudi Arabia, both larvae and adults of Oryctes are particularly troublesome, especially during the establishment of young date palm orchards. Endosymbiotic bacteria are known to have a key role in food digestion and insecticide resistance mechanisms, and therefore are essential to their host insect. Identification of these bacteria in their insect host can lead to developme...

  4. First record of Rhoptrocentrus piceus Marshall (Hymenoptera, Braconidae, Doryctinae as parasitoid of Psacothea hilaris hilaris (Pascoe (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Augusto Loni

    2015-02-01

    Full Text Available The species Rhoptrocentrus piceus Marshall (Hymenoptera: Braconidae was reared from the larvae of the xylophagous beetle Psacothea hilaris hilaris (Pascoe (Coleoptera: Cerambycidae, an exotic pest of Ficus and Morus species native to eastern Asia. It was recorded in the north of Italy in September 2005. This discovery is the first report of this species as parasitoids of the yellow spotted longicorn beetle all over the world.

  5. First record of Rhoptrocentrus piceus Marshall ( Hymenoptera , Braconidae , Doryctinae ) as parasitoid of Psacothea hilaris hilaris (Pascoe) ( Coleoptera , Cerambycidae )

    OpenAIRE

    Augusto Loni; Costanza Jucker; Sergey Belokobylskij; Daniela Lupi

    2015-01-01

    Abstract The species Rhoptrocentrus piceus Marshall ( Hymenoptera : Braconidae ) was reared from the larvae of the xylophagous beetle Psacothea hilaris hilaris (Pascoe) ( Coleoptera : Cerambycidae ), an exotic pest of Ficus and Morus species native to eastern Asia. It was recorded in the north of Italy in September 2005. This discovery is the first report of this species as parasitoids of the yellow spotted longicorn beetle all over the world.

  6. EFECTO DE LA GLUCOSA Y NITRATO DE AMONIO SOBRE LAS ENZIMAS LIGNINOLÍTICAS PRODUCIDAS POR Trametes versicolor INMOVILIZADO EN ESPUMA Y LA DECOLORACIÓN DE UN EFLUENTE PAPELERO EN UN BIORREACTOR DE LECHO FLUIDIZADO

    Directory of Open Access Journals (Sweden)

    M. Martínez-Salgado

    2005-12-01

    Full Text Available En Colombia se han realizado varios estudios para el tratamiento de aguas residuales de la industria papelera a escala de laboratorio, con el hongo Trametes versicolor inmovilizado en espuma de poliuretano; este hongo ha sido ampliamente estudiado por la producción de enzimas ligninolíticas y su potencial uso en la decoloración de efluentes. En este trabajo, se estudió el efecto de una fuente de carbono (glucosa y una de nitrógeno (nitrato de amonio en un biorreactor de lecho fluidizado, con adición al medio de cultivo de 0.02% (v/v de Tween 80, 12 ppm de MnSO4 y 1 mM de CuSO4, desarrollando un diseño factorial 22 con dos diferentes concentraciones de los nutrientes, siendo éstos las variables independientes y las variables dependientes fueron la actividad enzimática, las unidades de color y el consumo de sustrato los cuales fueron determinados por métodos colorimétricos, además de la biomasa y pH. El uso del biorreactor de lecho fluidizado con entrada de aire constante, arrojó datos importantes sobre la decoloracióny la producción de enzimas en el agua residual no estéril. Los máximos valores de la enzima Mnperoxidasa fueron hallados en las concentraciones altas y bajas de glucosa y nitrato de amonio con valores de 9.257U y 6.502U; para la enzima Lacasa, al adicionar separadamente las dos variables se obtuvo valores de 8.618U y 8.341U. Las enzimas fueron relacionadas con la disminución de las unidades de color hasta 481.06UC, junto con el aumento de la biomasa del hongo Trametes versicolor hasta 3.365 g junto con flora nativa del agua residual. Los resultados encontrados, determinaron que las variables de consumo de sustrato y biomasa, tienen una gran importancia debido a que el aumento de la biomasa genera un aumento en la actividad de la enzima MnP y en la reducción de las unidades de color.

  7. Ground beetle (Coleoptera: Carabidae) assemblages in narrow hedgerows in a Danish agricultural landscape

    DEFF Research Database (Denmark)

    Lövei, G. L.; Magura, T.

    2015-01-01

    The role of hedgerows in supporting ground beetles (Coleoptera: Carabidae) in a Danish agricultural landscape was examined. Nine old, well established single-row hedges were selected for the study, three each of a native species (hawthorn, Crataegus monogyna), a non-native deciduous one (rowan...... beetle assemblages. The number of ground beetle individuals and species were significantly the highest in the hawthorn hedges and significantly decreased from the hedges with rowan toward the spruce hedges. The elevated number of ground beetle individuals and species in the hawthorn hedges were due...... to the forest specialist species, as the number of forest specialist ground beetle individuals and species were significantly higher in the hawthorn hedges compared to the hedges with rowan and spruce. Differences in the number of the grassland and the cropland specialist ground beetle individuals and species...

  8. A importância forense de Oxelytrum discicolle (Brullé, 1840) (Coleoptera, Silphidae)

    OpenAIRE

    Camila Kraiczyi Kotzko; V. Costa-Silva; E. G. Silva

    2015-01-01

    Oxelytrum discicolle Brullé, 1840 é a espécie mais coletada de Silphidae (Coleoptera) na América Latina, constituindo um indicador forense na região Neotropical. O objetivo do trabalho é reunir informações sobre biologia e distribuição de O. discicolle, além de ferramentas para utilização desse besouro como indicador forense em casos de morte de humanos. O. discicolle é um besouro de grande porte, de cor preta com um colar vermelho. Larvas da espécie são necrófagas, já os adultos são onívoros...

  9. The first complete mitochondrial genome of stag beetle from China, Prosopocoilus gracilis (Coleoptera, Lucanidae).

    Science.gov (United States)

    Wu, Yuan-Yuan; Cao, Yu-Yan; Fang, Jie; Wan, Xia

    2016-07-01

    The complete mitochondrial genome (mitogenome) of Prosopocoilus gracilis (Coleoptera: Lucanidae) that is endemic to Southern China is determined. The circular genome is 736 bp in length and comprises 13 protein-coding genes, 22 tRNA, 2 rRNA genes and a control region. Gene order is identical to that of the putative ancestral arrangement of insects. The nucleotide composition of heavy strand is A (36.6%), C (22.6%), T (29.5%) and G (11.3%). All protein-coding genes start with a typical ATN codon except for the gene COI that uses AAC as the start codon. tRNA-Ser (AGN) uses the anticodon UCU instead of the commonly used GCU. Both maximum likelihood and Bayesian analyses support the monophyly of Lucanidae and the sister relationship of Nigidionus and the remaining sampled genera. Two species of Prosopocoilus were not recovered as a monophyletic group. PMID:26024142

  10. Estudos bioecológicos de Syphraea uberabensis (Coleoptera: Chrysomelidae Bechyné 1956

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    Charles Wikler

    2005-06-01

    Full Text Available Tibouchina herbacea (DC. Cog. é uma planta ornamental introduzida propositadamente no arquipélago do Havaí e, devido à ausência de inimigos naturais e condições edafo-climáticas adequadas, está se dispersando rapidamente pelas florestas nativas e regiões úmidas das principais ilhas havaianas. Por ser originária do Brasil, foram realizadas diversas viagens exploratórias em busca de por inimigos naturais específicos que pudessem controlar esta planta. Dentre os agentes selecionados com impacto e potencial de especificidade à T. herbacea encontra-se Syphraea uberabensis (Bechyne, 1955 (Coleoptera: Chrysomelidae. Este trabalho visa estudos sobre a biologia, ecologia e especificidade deste inseto e também uma avaliação criteriosa dos possíveis impactos causados por este inimigo natural na população da planta.

  11. The mortality of Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae induced by powdered plants

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    Kłyś Małgorzata

    2015-01-01

    Full Text Available The aim of this study was to investigate whether powdered plants of different species namely: peppermint Mentha piperita (L. (Lamiaceae, wormwood Artemisia absinthium (L. (Asteraceae, common sage Salvia officinalis (L. (Lamiaceae, allspice Pimenta dioica (Linnaeus et Merrill (Myrtaceae and common garlic Allium sativum (L. (Amaryllidaceae, added to semolina using concentrations of 1.23, 3.61, and 5.88%, influence the mortality rate in the saw-toothed grain beetle Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae. Experiments were conducted in a laboratory at 28°C and relative humidity 60±5%. At the concentration of 1.23%, allspice seeds caused the highest mortality amongst the saw-toothed grain beetle. When concentrations of 3.61 and 5.88% were used, sage, peppermint and wormwood caused the highest statistically significant mortality of O. surinamensis

  12. Communication disruption using synthetic sex pheromone for control of sugarcane wireworm. Melanotus okinawensis Ohira (Coleoptera: Elateridae)

    International Nuclear Information System (INIS)

    We tested communication disruption through the use of a synthetic sex pheromone from mid February to early June 2001 at the cultivating land on Minami-Daito Island in order to control the sugarcane wireworm Melanotus okinawensis Ohira (Coleoptera: Elateridae). Although very few males were caught in monitoring traps except a few traps set in a treated area, a large number were caught in traps set in an untreated Japanese pampas grass field. This indicates that communication disruption was effective throughout the period in the treated area. Females collected from treated area of Minami-Daito Island, mating rates that were lower than those of females in the untreated areas on Miyagi Island. We therefore concluded that a communication disruption system is an effective tool for the sugarcane wireworm management. (author)

  13. What do we know about winter active ground beetles (Coleoptera, Carabidae in Central and Northern Europe?

    Directory of Open Access Journals (Sweden)

    Radomir Jaskula

    2011-05-01

    Full Text Available This paper summarizes the current knowledge on winter active Carabidae in Central and Northern Europe. In total 73 winter active species are listed, based on literature and own observations. Ground beetles are among the three most numerous Coleoptera families active during the autumn to spring period. The winter community of Carabidae is composed both of larvae (mainly autumn breeding species and adults, as well as of epigeic species and those inhabiting tree trunks. Supranivean fauna is characterized by lower species diversity than the subnivean fauna. The activity of ground beetles decreases in late autumn, is lowest during mid-winter and increases in early spring. Carabidae are noted as an important food source in the diet of insectivorous mammals. They are also predators, hunting small winter active invertebrates.

  14. Synthesis, antifeedant activity against Coleoptera and 3D QSAR study of alpha-asarone derivatives.

    Science.gov (United States)

    Łozowicka, B; Kaczyński, P; Magdziarz, T; Dubis, A T

    2014-01-01

    For the first time, a set of 56 compounds representing structural derivatives of naturally occurring alpha-asarone as an antifeedants against stored product pests Sitophilus granarius L., Trogoderma granarium Ev., and Tribolium confusum Duv., were subjected to the 3D QSAR studies. Three-dimensional quantitative structure-activity relationships (3D-QSAR) for 56 compounds, including 15 newly synthesized, were performed using comparative molecular field analysis s-CoMFA and SOM-CoMSA techniques. QSAR was conducted based on a combination of biological activity (against Coleoptera larvae and beetles) and various geometrical, topological, quantum-mechanical, electronic, and chromatographic descriptors. The CoMSA formalism coupled with IVE (CoMSA-IVE) allowed us to obtain highly predictive models for Trogoderma granarium Ev. larvae. We have found that this novel method indicates a clear molecular basis for activity and lipophilicity. This investigation will facilitate optimization of the design of new potential antifeedants. PMID:24601760

  15. Taxonomy of Colophon Gray (Coleoptera: Lucanidae): new species and a status change.

    Science.gov (United States)

    Jacobs, Carmen T; Scholtz, Clarke H; Strümpher, Werner P

    2015-01-01

    Three new species of the Cape high-mountain stag beetle genus, Colophon Gray (Coleoptera: Lucanidae), from South Africa are described. They are C. deschodti new species, C. switalae new species, and C. struempheri new species. The new taxa fall within a species complex of geographically disjunct entities related to Colophon stokoei Barnard. Furthermore, the mitochondrial COI gene shows a high degree of sequence divergence, with pairwise genetic distances between the species ranging between 7.4-10.7%. The new species are illustrated by photographs. Colophon eastmani nagaii Mizukami is raised to species level on the basis of geographic range and molecular differences between it and the nominate subspecies. This brings the total number of described species in the genus to 21. An updated checklist of the South African species of Colophon is also provided. PMID:26701471

  16. Carpophilus zeaphilus, a new sap beetle species acclimatized in Italy (Coleoptera: Nitidulidae

    Directory of Open Access Journals (Sweden)

    Paolo Audisio

    2015-06-01

    Full Text Available Carpophilus zeaphilus Dobson, 1969 (Coleoptera, Nitidulidae, Carpophilinae is an Afrotropical species that has become widespread in Sub-Saharan Africa, the Arabian Peninsula, and southern Mediterranean areas in recent years. The species was first recorded from Europe in Portugal and Spain nearly thirty years ago, and it was later intercepted in Sicily near Trapani in 1991. A few specimens of this species were collected in April, 2015 in a sparsely forested area near Rome, which suggests a recent acclimatization into peninsular Italy. Specimens were taken on flowering trees of Prunus spinosa L. (Rosaceae, an unusual occurrence for most introduced species of Carpophilinae that are normally associated with rotten fruit and other decomposing vegetal matter.

  17. Diversidade de Carabidae (Coleoptera) Amostrados em Áreas de Reflorestamento de Mata Ciliar e Fragmento Florestal, no Estado do Paraná

    OpenAIRE

    Tamara Quinteiro; José Lopes; Ivan Martins

    2012-01-01

    Carabidae são Coleoptera com a maioria de suas espécies apresentando hábito alimentar predatório. O objetivo do presente trabalho foi estudar a diversidade e abundância da família Carabidae (Coleoptera) em áreas de fragmento florestal e em áreas de reflorestamento de mata ciliar, evidenciando a importância deste grupo como indicador da biodiversidade em relação ao sucesso do reflorestamento. Os besouros foram amostrados por meio de armadilhas de solo pitfall, em fragmentos florestais e reflor...

  18. Isolation,Purification and Structural Characterization of an Aqueous Polysacc-haride from Natural Coriolus versicolor%野生云芝中水溶性多糖的分离鉴定

    Institute of Scientific and Technical Information of China (English)

    陈海生; 梁荣能; 徐一新

    2000-01-01

    目的:进一步研究野生云芝(Coriolus versicolor (L.) Fr.)子实体中具有生物活性的多糖成分.方法:采用水提醇沉淀法得粗多糖,经H2O2脱色,醇分部沉淀,SephadexG75和HPLC纯化,GC和光谱测定糖组成及糖链结构.结果:从野生云芝中分离纯化了一种水溶性多糖CVPS-B,分子量39kd,其单糖组成及摩尔比为:Glc,Fuc,Ara,Man,Gal,13.647∶5.882∶4.452:2.425∶1.000;其多糖主链为β(1-3)葡聚糖,支链上含有一定量的夫糖(fucose).结论:CVPS-B为云芝中新发现的一组分多糖,与日本培养的云芝多糖PS-K类似,但不含多肽,而与上海师范大学培养的云芝多糖PSP有所不同.

  19. Locomotor activity of Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae on Chilean sandy beaches Actividad locomotora de Phalerisida maculata Kulzer (Coleoptera, enebrionidae en playas arenosas chilenas

    Directory of Open Access Journals (Sweden)

    EDUARDO JARAMILLO

    2000-03-01

    Full Text Available The locomotor activity of the beetle Phalerisida maculata Kulzer (Coleoptera Tenebrionidae was studied on the surface of the substrate in two sandy beaches of the Chilean coast: one in north central (ca. 29ºS and the other in south central Chile (ca.39ºS. During the summer period of 1991 the circadian locomotor activity was studied in the southern beach, while during that of 1997 in both beaches. To analyze the activity, pitfall traps were used which were ordered along two transects extended between the upper beach and the resurgence zone. The traps were checked (i.e. collection of captured insects every two hours for a total period of 26 hours. The results showed that the adults of P. maculata were mostly active during the night hours, whereas the larvae were active during both, the day and night. Studies carried out in the beach located in south central Chile show that differences in the tidal range (neap vs. spring tides do not affect the activity patterns. During the locomotor activity, adult and larvae move to lower intertidal levels than those usually occupied while buried. Results of laboratory experiments using actographs under conditions of darkness and constant temperature, suggest that adults and larvae of P. maculata presented a circadian rhythm similar to that observed in the field experiments. It is concluded that P. maculata presents a behaviour that appear to be under control of an endogenous rhythm, without showing differences in the circadian rhythm of activity when beaches located at different latitudes are comparedSe estudió la actividad locomotriz del escarabajo Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae sobre la superficie del sustrato de dos playas arenosas de la costa de Chile: una en el centro norte (ca. 29ºS y otra en el centro sur (ca. 39ºS. Durante el período estival de 1991 se estudió la actividad locomotriz circadiana en la playa del centro sur y durante el de 1997 en ambas playas. Para analizar

  20. Nuevas citas de Coleoptera para la Argentina (Carabidae, Lucanidae, Scarabaeidae y Tenebrionidae New records of Coleoptera for Argentina (Carabidae, Lucanidae, Scarabeidae and Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sergio Roig-Juñent

    2004-12-01

    Full Text Available En enero de 2003, se realizó un viaje de campaña con el fin de recolectar materiales en áreas poco prospectadas de la estepa patagónica de Mendoza y Neuquén, Argentina, así como en ambientes de bosques de Nothofagus en la provincia de Neuquén. Las recolecciones se realizaron en forma manual, usando trampas con cebo y trampas de luz de mercurio. El material recolectado permite citar por primera vez para la Argentina los géneros Nothobroscus Roig-Juñent & Ball y Mimophilorizus Mateu (Carabidae, Callyntra Solier y Homocyrtus Reitter (Tenebrionidae. Además, se citan por primera vez para la Argentina las especies Cnemalobus sulciferus Philippi (Carabidae, Allidiostoma landbecki (Philippi, Macrodactylus chilensis Solier y Brachysternus marginatus (Germain (Scarabeidae y Pycnosiphorus philippii (Westwood (Lucanidae. Se provee una breve descripción de los ambientes en donde estas especies fueron encontradas.A short expedition to northern Patagonia was made in January 2003. The main goal of the trip was to survey the diversity of some families of Coleoptera in the insufficient explored steppes in Mendoza and Neuquén provinces, Argentina and Nothofagus forests of Neuquén province. The techniques included manual collection and the use of different kinds of traps, such as with bite or artificial light of mercury. The study of the material revealed four new genera records and four new species records for the country: Nothobroscus Roig-Juñent & Ball and Mimophilorizus Mateu (Carabidae, Callyntra Solier and Homocyrtus Reitter (Tenebrionidae are the new genus records. Cnemalobus sulciferus Philippi (Carabidae, Allidiostoma landbecki (Philippi, Macrodactylus chilensis Solier, and Brachysternus marginatus (Germain (Scarabeidae, and Pycnosiphorus philippii (Westwood (Lucanidae are the new species records. A brief description of the habitats where the species were collected is provided.

  1. Immunofluorescence localization and ultrastructure of Stewart’s wilt disease bacterium Pantoea stewartii in maize leaves and in its flea beetle vector Chaetocnema pulicaria (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Pantoea stewartii is the causal agent of Stewart's wilt of sweet corn, the most serious bacterial disease of sweet corn and maize in the North-Central and Eastern USA. P. stewartii is transmitted mainly by the corn flea beetle Chaetocnema pulicaria (Coleoptera: Chrysomelidae) and this bacterium is a...

  2. Preliminary evaluation of Delphastus catalinae (Coleoptera:Coccinellidae)as a predator of the ficus whitefly, Singhiella simplex (Singh) (Hemiptera:Aleyrodidae)

    Science.gov (United States)

    The predatory lady beetle Delphastus catalinae (Horn) (Coleoptera: Coccinellidae) was evaluated as a potential biological control agent against the ficus whitefly, Singhiella simplex (Singh) (Hemiptera: Aleyrodidae), a newly-invasive pest of ficus plants. Adult D. catalinae females were starved for ...

  3. Brood production by Xylosandrus germanus (Coleoptera: Curculionidae) and growth of its fungal symbiont on artificial diet based on sawdust of different species

    Science.gov (United States)

    The ambrosia beetle Xylosandrus germanus (Blanford) (Coleoptera: Curculionidae) is among the most important exotic pests of orchards and nurseries in the US. It attacks a wide range of hosts and is difficult to control using conventional insecticides. As part of our studies on the biology and cont...

  4. A new genus and species of myrmecophilous aphodiine beetle (Coleoptera, Scarabaeidae inhabiting the myrmecophytic epiphyte Platycerium sp. (Polypodiaceae in the Bornean rainforest canopy

    Directory of Open Access Journals (Sweden)

    Munetoshi Maruyama

    2010-01-01

    Full Text Available Pterobius itiokai Maruyama, gen. n. and sp. n., (Coleoptera: Scarabaeidae: Aphodiinae is described from Lambir Hills National Park, Borneo based on specimens collected from a Crematogaster difformis ant nest in the myrmecophytic epiphytic fern genus Platycerium. Pterobius belongs to the tribe Eupariini and is closely related to the Indo-Australian genus Cnematoplatys.

  5. Assembly and annotation of full mitochondrial genomes for the corn rootworm species, Diabrotica virgifera virgifera and D. barberi (Insecta: Coleoptera: Chrysomelidae), using Next Generation Sequence data

    Science.gov (United States)

    Complete mitochondrial genomes for two corn rootworm species, Diabrotica v. virgifera (16,747 bp) and D. barberi (16,632; Insecta: Coleoptera: Chrysomelidae), were assembled from Illumina HiSeq2000 read data. Annotation indicated that the order and orientation of 13 protein coding genes (PCGs), and...

  6. New records of Paracrias Ashmead (Hymenoptera, Eulophidae as parasitoids on weevil larvae (Coleoptera, Curculionidae in Brazil, with the description of a new species

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    Luciano Palmieri

    2013-09-01

    Full Text Available Paracrias strii Schauff, 1985 and P. ceratophaga Palmieri & Hansson sp. nov. are first record in Brazil and both are associated with Ceratopus Schoenherr larvae (Coleoptera, Curculionidae reared from syconia of two species of fig-trees. Both Paracrias species are diagnosed and illustrated. Males of P. ceratophaga sp. nov. are described. The association of Paracrias with weevil larvae is briefly discussed.

  7. Biology of two members of the Euwallacea fornicatus species complex (Coleoptera: Curculionidae: Scolytinae), recently invasive in the USA, reared on an ambrosia beetle artificial diet

    Science.gov (United States)

    1. Diet and rearing protocols were developed for two members of the cryptic Euwallacea fornicatus species complex, polyphagous shot hole borer (PSHB) and tea shot hole borer (TSHB) (Coleoptera: Curculionidae: Scolytinae), using sawdust from boxelder Acer negundo and avocado Persea americana. 2. Bio...

  8. Releases, distribution and abundance of Gratiana boliviana (Coleoptera:Chrysomelidae), a biological control agent of tropical soda apple (Solanum viarum, Solanaceae), in Florida

    Science.gov (United States)

    A biological control program against tropical soda apple (TSA) (Solanum viarum Dunal (Solanaceae)) released 176,643 Gratiana boliviana Spaeth (Coleoptera: Chrysomelidae) in Florida from 2003 to 2008. The spatial distribution of releases was clustered with more beetles released in south/central Flor...

  9. Efeitos da radiação gama do Cobalto-60 em ovos de Tribolium castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae) Gamma radiation effects on eggs of Tribolium castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae)

    OpenAIRE

    L. S. Fontes; V. Arthur

    1994-01-01

    Este trabalho teve por objetivo determinar as doses esterilizantes e letal para ovos de T. castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae), através de doses crescentes de radiação gama. Utilizou-se uma fonte de Cobalto-60, tipo Gammabeam-650, com taxa de dose de 1,28 kGy/hora. O experimento foi conduzido sob condições controladas com temperatura de 25 ± 2°C e umidade relativa de 70 ± 5%. As doses letal e esterilizante para os ovos foram respectivamente 30 e 20 Gy.The o...

  10. Desenvolvimento de Podisus nigrispinus (Dallas (Heteroptera, Pentatomidae com Zophobas confusa Gebien (Coleoptera, Tenebrionidae comparado à duas outras presas alternativas Development of Podisus nigrispinus (Dallas (Heteroptera, Pentatomidae fed with Zophobas confusa Gebien (Coleoptera, Tenebrionidae compared with two another alternative preys

    Directory of Open Access Journals (Sweden)

    Teresinha V Zanuncio

    1996-01-01

    Full Text Available Nymphs of Podisus nigrispinus (Dallas, 1851 were fed with Zophobas confusa Gebien, 1906, Tenebrio molitor Linnaeus, 1758 (Coleoptera, Tenebrionidae and Musca domestica Linnaeus, 1758 (Diptera, Muscidae under 25±0.5ºC, 60±10% of RH and photophase of 12h. Nymphal viability of P. nigrispinus were 64.0%, 80.0% and 92.0% with Z. confusa, M. domestica and T. molitor, respectively. No differences were found for pre-oviposition period, number of egg masses, number of eggs, egg viability, and longevity for females of this predator fed with any of these preys.

  11. Tipos de Curculionidae (Coleoptera: Curculionoidea) depositados en la colección entomológica del Museo de La Plata Types of Curculionidae (Coleoptera: Curculionoidea) housed at the Museo de La Plata entomological collection

    OpenAIRE

    Analía A. Lanteri; Suárez, Sonia M.; Guadalupe del Río, M.

    2003-01-01

    Esta contribución referida al material tipo de Curculionidae (Coleoptera: Curculionoidea) depositado en la colección entomológica del Museo de La Plata, actualiza la información sobre los tipos depositados durante los últimos diez años. Los 111 tipos examinados en este trabajo corresponden a 52 especies asignadas a las siguientes subfamilias: Curculioninae (5 spp.), Cryptorhynchinae (8 spp.), Cyclominae (4 spp.), Entiminae (31 spp.), y Erirhininae (4 spp.). Los autores de las especies son C. ...

  12. 从花斑糠疹皮损分离的马拉色菌随机扩增多态性DNA研究%Random amplified polymorphic DNA analysis of Malassezia isolates from cutaneous lesions of pityriasis versicolor

    Institute of Scientific and Technical Information of China (English)

    谢震; 冉玉平; 刘瑞; 杨如学; 李志瑜; 代亚玲

    2009-01-01

    目的 用随机扩增多态性DNA方法 研究分离自花斑糠疹的马拉色菌种间和株间差异,了解随机扩增多态性DNA分析(RAPD)与生理生化方法 在菌种分型上的差异及菌株DNA型别和菌种间的关系.方法 用氯化苄法提取马拉色菌标准株(10株7个种)和临床分离株(47株)的基因组DNA,其中临床株分离自34例花斑糠疹患者,经形态学和生理生化方法 鉴定为5个种(合轴马拉色菌、糠秕马拉色菌、钝形马拉色菌、球形马拉色菌、限制马拉色菌),用4种随机引物(S22、SS24、S25、S33)对菌株DNA做PCR随机扩增,NTSYS软件自动生成树状分支图.结果 绝大多数标本均可被4种引物扩增而获得清晰条带,其中2种引物(S22、S24)的条带更为稳定、清晰.共82条DNA片段被扩增,所有菌株均可见种间和株间多态性.有4例患者皮损同时分离出不同种的菌株显示遗传相似性高,在树状图中归入一类.结论 来自同一宿主的不同菌株遗传趋同现象提示马拉色菌的种特异性、菌种演化与宿主间存在密切关系.%Objective To investigate intraspecific and interspecific variation within Malassezia iso-lates from patients with pityriasis versicolor by random amplified polymorphic DNA (RAPD) analysis, to learn the difference between RAPD analysis and physiological and biochemical methods in the typing of Malassezia species, and to explore the relationship between RAPD patterns and Malassezia species. Methods A total of 47 Malassezia isolates were obtained from 34 patients with pityriasis versicolor, and they were classified into 5 species by morphological, physiological and biochemical features, I.e., M. Fin'fur, M. Obtusa, M. Globosa, M. Restricta and M. Sympodialis. Genomic DNA was extracted from the 47 clinical isolates and 10 reference strains (including 7 species) of Malassezia. PCR was performed using 4 random primers including S22, S24, S25 and S33. RAPD patterns were analyzed by NTSYS

  13. Influence of 60Co γ irradiation on fertility of Japanese pine sawyer beetle Monochamus alternatus hope (Coleoptera: Cerambycidae)

    International Nuclear Information System (INIS)

    The fertility of the Japanese pine sawyer beetle Monochamus alternatus (Coleoptera: Cerambycidae) irradiated with 60Co γ-rays was remarkable reduced at the doses of 30Gy, 35Gy and 40Gy, especially at 40Gy. When the non-irradiated females were coupled with the irradiated males first, and then coupled with non-irradiated males, the hatchability and the fertility had little higher but lower than the control. It explained that radiation has certain influence to the female gonad. It also has difference between the hatching rate and the amount of eggs in different match. (authors)

  14. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus.

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  15. Coleoptera Scarabaeoidea de la región de Teziutlán, Puebla, México

    OpenAIRE

    Adrián MUÑOZ-HERNÁNDEZ; Morón, Miguel Angel; Agustín ARAGÓN

    2008-01-01

    Con el propósito de evaluar la riqueza, abundancia y fenología de las especies de Coleoptera Scarabaeoidea o Lamellicornia de la región de Teziutlán, Puebla, se realizaron colectas mensuales entre mayo de 1999 y junio de 2000, en sitios con bosques de pino-encino-liquidámbar o zonas de cultivo situados entre 1,990 y 2,000 m de altitud. Se capturaron 2,082 ejemplares que representan a 59 especies de 29 géneros, 18 tribus y 7 subfamilias de Melolonthidae, Scarabaeidae y Passalidae, entre las qu...

  16. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    Abstract A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  17. Molecular identification of Epitrix potato flea beetles (Coleoptera: Chrysomelidae) in Europe and North America.

    Science.gov (United States)

    Germain, Jean-François; Chatot, Catherine; Meusnier, Isabelle; Artige, Emmanuelle; Rasplus, Jean-Yves; Cruaud, Astrid

    2013-06-01

    Epitrix species (Coleoptera: Chrysomelidae) feed mostly on plants from the family Solanaceae and some of them are major pests of potato crops. All Epitrix species are morphologically highly similar, which makes them difficult to identify and limits their study and management. Identification of species is mostly based on the observation of the genitalia and requires a high level of expertise. Here, we propose a tool to reliably identify all developmental stages of the most economically important Epitrix species feeding on potato in Europe and North America (Epitrix cucumeris, Epitrix similaris, Epitrix tuberis, Epitrix subcrinita and Epitrix hirtipennis). We first sequenced two DNA markers (mitochondrial cytochrome c oxidase I (COI) and nuclear internal transcribed spacer 2 (ITS2)) to test their effectiveness in differentiating among six Epitrix species (126 specimens). Morphospecies of Epitrix were well-differentiated by both DNA barcodes and no mitochondrial introgression was detected. Then, we developed an RFLP-based diagnostic method and showed that unambiguous species discrimination can be achieved by using the sole restriction enzyme TaqI on COI polymerase chain reaction products. The tool proposed here should improve our knowledge about Epitrix species biology, distribution and host range, three capacities that are particularly important in the detection and management of these pest species. Specifically, this tool should help prevent the introduction of E. tuberis and E. subcrinita in Europe and limit the spread of the recently introduced E. cucumeris and E. similaris, with minimal disruption to Solanaceae trade. PMID:23448201

  18. Sustainable management tactics for control of Phyllotreta cruciferae (Coleoptera: Chrysomelidae) on canola in Montana.

    Science.gov (United States)

    Reddy, Gadi V P; Tangtrakulwanich, Khanobporn; Miller, John H; Ophus, Victoria L; Prewett, Julie

    2014-04-01

    The crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae), has recently emerged as a serious pest of canola (Brassica napus L.) in Montana. The adult beetles feed on canola leaves, causing many small holes that stunt growth and reduce yield. In 2013, damage to canola seedlings was high (approximately 80%) in many parts of Montana, evidence that when flea beetles emerge in large numbers, they can quickly destroy a young canola crop. In the current study, the effectiveness of several biopesticides was evaluated and compared with two insecticides (deltamethrin and bifenthrin) commonly used as foliar sprays as well as seed treatment with an imidacloprid insecticide for the control of P. cruciferae under field conditions in 2013. The biopesticides used included an entomopathogenic nematode (Steinernema carpocapsae), two entomopathogenic fungi (Beauveria bassiana and Metarhizium brunneum), neem, and petroleum spray oils. The control agents were delivered in combination or alone in a single or repeated applications at different times. The plant-derived compound neem (azadirachtin), petroleum spray oil, and fatty acids (M-Pede) only showed moderate effect, although they significantly reduced leaf injuries caused by P. cruciferae and resulted in higher canola yield than the untreated control. Combined use of B. bassiana and M. brunneum in two repeated applications and bifenthrin in five applications were most effective in reducing feeding injuries and improving yield levels at both trial locations. This indicates that entomopathogenic fungi are effective against P. cruciferae, and may serve as alternatives to conventional insecticides or seed treatments in managing this pest. PMID:24772547

  19. Erotylidae (Insecta, Coleoptera of Poland – problematic taxa, updated keys and new records

    Directory of Open Access Journals (Sweden)

    Rafal Ruta

    2011-10-01

    Full Text Available New data concerning the occurrence of pleasing fungus beetles (Coleoptera: Erotylidae in Poland are given, with a focus on rare and difficult to identify Central European taxa. Cryptophilus cf. integer (Heer (Cryptophilinae is reported from the Polish territory for the first time based on adult and larval specimens collected in the Wielkopolska-Kujawy Lowland. Identification problems concerning species of Cryptophilus introduced to Europe are discussed. Triplax carpathica Reitter (Erotylinae is recorded from the Białowieża Primeval Forest, which is the first known non-Carpathian finding of this species, located in the close proximity of the Polish-Belarussian UNESCO World Heritage Site “Białowieża Forest”. Discussion of T. carpathica being conspecific with Siberian T. rufiventris Gebler is provided. New Polish localities of several other Erotylidae are reported, and an updated key to Central European species of Triplax is given. The Triplax key is supplemented with dorsal and ventral habitus images of all treated Triplax species. One of the rarest Central European erotyline species Combocerus glaber (Schaller is recorded from xerothermic grasslands in North-West Poland.

  20. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect

    International Nuclear Information System (INIS)

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5–7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1–4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. (author)

  1. The complete mitochondrial genome of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Liu, Qiu-Ning; Bian, Dan-Dan; Jiang, Sen-Hao; Li, Zhen-Xing; Ge, Bao-Ming; Xuan, Fu-Jun; Yang, Li; Li, Chao-Feng; Zhang, Dai-Zhen; Zhou, Chun-Lin; Tang, Bo-Ping

    2016-01-01

    The complete mitochondrial genome (mitogenome) of Tribolium castaneum (Coleoptera: Tenebrionidae) was determined to be 15,883 bp (GenBank accession No. KM009121), which contains 22 tRNA genes, 13 protein-coding genes (PCGs), 2 rRNA genes and a major non-coding A + T-rich region. It has the typical gene organization and order of mitogenomes from ancestral insects. The nucleotide composition was also biased toward A + T nucleotides (71.72%) and the AT skew of this mitogenome was slightly positive. All of the 22 tRNA genes displayed a typical clover-leaf structure, with the exception of trnS1 (AGN). Thirteen PCGs were initiated by ATN codons, except for cytochrome c oxidase subunit 1 (cox1) gene which was initiated by AAT. Eight of the 13 PCGs harbor the incomplete termination codon by T or TA. The A + T-rich region of the mitogenome was 1237 bp in length and the A + T content was 82.30%. PMID:25162515

  2. Caracteres preimaginales y aspectos bionómicos de Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Jerez, V.

    2011-12-01

    Full Text Available We describe the morphology and microstructure of the egg chorion, and the morphology of the first instar larva of Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae, Nycteliini. Bionomical and distributional data on this species are also provided. To obtain eggs and larvae, couples were collected in the field and kept in rearing cages until oviposture and ecclosion. The structure and adornment of the egg exochorion, and the exterior morphological features of larvae were examined with electron scanning microscopy. The eggs of G. luczotii showed a rounded micropyle and a smooth exochorion, composed of hexagonal cells without aeropyles. The larvae of G. luczotii showed morphological characteristics suited for an edaphic life similar to that of Pedobionta: digging prothoracic legs, cephalic capsule with abundant sensilla, and well–developed pygopodium. We analysed the importance of larval morphology as an element for specific diagnosis and found that interspecific differences regarding frontal sensilla, clypeus shape, and anterior part of labrum, had a taxonomic value and possibly a phylogenetic value.

  3. Karyotype differentiation patterns in species of the subfamily Scarabaeinae (Scarabaeidae, Coleoptera).

    Science.gov (United States)

    Cabral-de-Mello, Diogo Cavalcanti; de Oliveira, Sárah Gomes; Ramos, Ituza Celeste; de Moura, Rita de Cássia

    2008-12-01

    The aim of this study was to describe the karyotype of species belonging to the subfamily Scarabaeinae (Coleoptera, Scarabaeidae) and to compile the conventional cytogenetic data available in the literature for this group. The karyotypes of ten species belonging to the tribes Canthonini, Coprini, Onthophagini and Phanaeini were analyzed by conventional staining. Eight of these species were described for the first time (Canthon aff carbonarius, Canthon chalybaeus, Coprophanaeus dardanus, Deltochilum aff amazonicum, Dichotomius geminatus, Oxysternon silenus, Phanaeus chalcomelas and Malagoniella aff astyanax) and two were redescribed (Diabroctis mimas and Digitonthophagus gazella) since their karyotypes differed from those previously published in the literature. Four species studied showed a diploid number of 2n=20 and a parachute type sex determining system and the karyotype was 2n=20,Xy in two species and 2n=18,Xy(p), 2n=19,X0, 2n=12,XY and 2n=14,neoXY in one each. The chromosome morphology of the different species varied, with the observation of metacentric, submetacentric, subacrocentric and acrocentric chromosomes. The X chromosome was predominantly meta or submetacentric in the species analyzed, whereas the y chromosome presented two arms or was punctiform. In conclusion, the subfamily Scarabaeinae comprises 120 species analyzed cytogenetically, and are observed the occurrence of five chromosome rearrangements (autosome-autosome and X-autosome fusions, pericentric inversions, fissions and loss of the y chromosome) that are related to the chromosome variability and evolution in the group. PMID:18495484

  4. Fruit Damage Patterns Caused by Ovipositing Females of Conotrachelus dimidiatus (Coleoptera: Curculionidae in Guava Trees

    Directory of Open Access Journals (Sweden)

    Felipe Tafoya

    2010-01-01

    Full Text Available We evaluated the damage patterns produced by females of the guava weevil Conotrachelus dimidiatus Champion, 1904 (Coleoptera: Curculionidae, according to the position of the damaged fruit in guava trees Psidium guajava L. in Calvillo, Aguascalientes, Mexico. The trees were subdivided in eight zones, and during one year the level of fruit lesions due to oviposition was registered. Results showed a higher level of damage in the upper and external zone of the trees (P≤.05. We found no significant differences in damage between the four cardinal points (P≥.05. During the year, the level of damage was recorded and was higher in the months of August and September (P≤.05 associated with rainfall (0.86, P=.06 and increase in temperature (0.84, P=.03. The most susceptible fruits were in the size range of 2.1–4.0 cm (polar diameter. The information from this study will be used to design and establish effective control strategies for the guava weevil, taking into account location of the most susceptible fruits, seasonality of the pest, and the abiotic factors.

  5. The Hydraulic Mechanism of the Unfolding of Hind Wings in Dorcus titanus platymelus (Order: Coleoptera

    Directory of Open Access Journals (Sweden)

    Jiyu Sun

    2014-04-01

    Full Text Available In most beetles, the hind wings are thin and fragile; when at rest, they are held over the back of the beetle. When the hind wing unfolds, it provides the necessary aerodynamic forces for flight. In this paper, we investigate the hydraulic mechanism of the unfolding process of the hind wings in Dorcus titanus platymelus (Oder: Coleoptera. The wing unfolding process of Dorcus titanus platymelus was examined using high speed camera sequences (400 frames/s, and the hydraulic pressure in the veins was measured with a biological pressure sensor and dynamic signal acquisition and analysis (DSA during the expansion process. We found that the total time for the release of hydraulic pressure during wing folding is longer than the time required for unfolding. The pressure is proportional to the length of the wings and the body mass of the beetle. A retinal camera was used to investigate the fluid direction. We found that the peak pressures correspond to two main cross-folding joint expansions in the hind wing. These observations strongly suggest that blood pressure facilitates the extension of hind wings during unfolding.

  6. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae

    Directory of Open Access Journals (Sweden)

    Andréa Roveré Franz

    2011-03-01

    Full Text Available Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae. Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf, Zingiber officinale (root and Mentha sp. (leaf. The efficacy of these oils was tested to control the rice weevil, S. oryzae, using hydrodistillation. Chemical analysis of the essential oils was carried out by gas chromatography. Major components of C. citratus were geranial (48% and neral (31%, of Z. officinale were α-zingibereno (13%, geranial (16%, neral (10% and α-farneseno (5% and of Mentha sp. was menthol (92%. Bioassays were carried out by fumigation and topical application. In topical application assays, the essential oil of C. citratus had greater toxicity (LC50 0.027 µL mL-1 and shorter exposure time than the oils of the other two plants. After 24 h and 48 h, 70% and 100% mortality of S. oryzae occurred, respectively. In fumigation assays, essential oil of Z. officinale had a lower LC50 (1.18 µL cm-2 and 70% mortality after 24 h exposure. Therefore, we recommend the use of essential oils of C. citratus and Z. officinale to control the rice weevil S. oryzae.

  7. On the spatial spread of the Rice Water Weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Erirhinidae, in Italy

    Directory of Open Access Journals (Sweden)

    Daniela Lupi

    2010-07-01

    Full Text Available A five year study has been made to establish the spread of the rice water weevil Lissorhoptrus oryzophilus (Coleoptera: Erirhinidae in Northern Italy. Data obtained with GPS from 2005 throughout 2009 were first georeferenced with SW ArcGis® 9.2, then overlapped and compared to the map of the European environmental landscape based on the interpretation of satellite images (CORINE Land Cover map and to the hydrographic chart CT10 (Technical Regional map 10000. The analysis of the radial rate of spread per year indicates a deceleration in the expansion from 10.864 ± 6.801 km/year in 2005 to 5.318 ± 1.401 km/year in 2009. In five years the weevil has expanded its distribution in nearly all rice paddies in Lombardy and Piedmont, over an area of about 200,000 ha, which correspond to 86% of the total Italian rice area. Its expansion is thought to follow a type of stratified dispersal, due both to insect adult active dispersal and to accidental movements caused by human transportation.

  8. Naturally-Occurring Entomopathogenic Fungi on Three Bark Beetle Species (Coleoptera: Curculionidae in Bulgaria

    Directory of Open Access Journals (Sweden)

    Slavimira A. Draganova

    2010-01-01

    Full Text Available Bark beetles (Coleoptera: Curculionidae: Scolytinae belong to one of the most damaging groups of forest insects and the activity of their natural enemies –pathogens, parasitoids,parasites or predators suppressing their population density,is of great importance. Biodiversity of entomopathogenic fungi on bark beetles in Bulgaria has been investigated sporadically. The aim of this preliminary study was to find, identify and study morphological characteristics of fungal entomopathogens naturally-occurring in populations of three curculionid species – Ips sexdentatus Boern, Ips typographus (L. and Dryocoetes autographus (Ratz.. Dead pest adults were found under the bark of Pinus sylvestris and Picea abies trees collectedfrom forests in the Maleshevska and Vitosha Mountains. Fungal pathogens were isolated into pure cultures on SDAY (Sabouraud dextrose agar with yeast extract and were identified based on morphological characteristics both on the host and in a culture.Morphological characteristics of the isolates were studied by phenotypic methods. The fungal isolates obtained from dead adults of Ips sexdentatus, Ips typographus and D. autographus were found to belong to the species Beauveria bassiana (Bals. – Criv. Vuillemin,Beauveria brongniartii (Saccardo Petch and Isaria farinosa (Holmsk. Fries (anamorph Ascomycota, Sordariomycetes: Hypocreales, Cordycipitaceae. Morphological traits of the isolates are described.

  9. Pimenta pseudocaryophyllus Derivatives: Extraction Methods and Bioactivity Against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae).

    Science.gov (United States)

    Ribeiro, L P; Ansante, T F; Niculau, E S; Pavarini, R; Silva, M F G F; Seffrin, R C; Vendramim, J D

    2015-12-01

    Plant-based insecticides can play an important role in integrated insect pest management (IPM), especially in protecting stored grains. The aim of this study was to evaluate the bioactivity of derivatives (powder, ethanolic extract, and essential oil (EO)) from the leaves of Pimenta pseudocaryophyllus (Myrtaceae), a Brazilian native species, against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), the main insect pest of stored corn. The powder and essential oil prepared from leaves showed a repellent effect. Moreover, the EO exhibited promising insecticidal activity through residual contact (LC50 = 1522 mg kg(-1)) and significantly decreased the F 1 progeny and the percentage of damaged grains. However, the essential oil obtained from P. pseudocaryophyllus leaves did not result in significant mortality of S. zeamais adults after 72 h of exposure by fumigation in concentrations up to 400 μL L(-1) of air. Based on GC-MS analysis, 20 compounds were identified in the essential oil of P. pseudocaryophyllus leaves, being chavibetol (38.14%), methyl eugenol (11.35%), and terpinolene (9.17%) as the major constituents. Essential oil from P. pseudocaryophyllus leaves is an interesting source of compounds with grain-protectant properties and should be analyzed in future studies aiming to develop new bioinsecticides to use in the IPM of stored grains. PMID:26285912

  10. Coexistence and Competition between Tomicus yunnanensis and T. minor (Coleoptera: Scolytinae in Yunnan Pine

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    Rong Chun Lu

    2012-01-01

    Full Text Available Competition and cooperation between bark beetles, Tomicus yunnanensis Kirkendall and Faccoli and Tomicus minor (Hartig (Coleoptera: Scolytinae were examined when they coexisted together in living Yunnan pine trees (Pinus yunnanensis Franchet in Yunnan province in Southwest China. T. yunnanensis bark beetles were observed to initiate dispersal from pine shoots to trunks in November, while the majority of T. minor begins to transfer in December. T. yunnanensis mainly attacks the top and middle parts of the trunk, whereas T. minor mainly resides in the lower and middle parts of the trunk. The patterns of attack densities of these two species were similar, but with T. yunnanensis colonizing the upper section of the trunk and T. minor the lower trunk. The highest attack density of T. Yunnanensis was 297 egg galleries/m2, and the highest attack density of T. minor was 305 egg galleries/m2. Although there was significant overlap for the same bark areas, the two species generally colonize different areas of the tree, which reduces the intensity of competition for the relatively thin layer of phloem-cambium tissues where the beetles feed and reside.

  11. Factors Affecting Pheromone Production by the Pepper Weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae and Collection Efficiency

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    Fred J. Eller

    2014-11-01

    Full Text Available Several factors affecting pheromone production by male pepper weevils, Anthonomus eugenii Cano (Coleoptera: Curculionidae as well as collection efficiency were investigated. Factors studied included: porous polymer adsorbents (Tenax versus Super Q, male age, time of day, male density, and male diet. Super Q was found to be a superior adsorbent for the male-produced alcohols and geranic acid as well as the plant-produced E-β-ocimene. Pheromone production increased with male age up to about age 15 days old and then tapered off. Male pepper weevils produced the highest amount of pheromone between noon and 2 pm (i.e., 4 to 6 h after “lights on” and were producing ca. 800 ng/h during this period. Thereafter, pheromone production decreased and was extremely low during the scotophase (i.e., ca. 12 ng/h. Male pepper weevil density had a significant effect on both release rate and pheromone composition. Pheromone production on a per male basis was highest for individual males and the percentage of geranic acid in the blend was lowest for individual males. Male pepper weevils produced only extremely low amounts of pheromone when feeding on artificial diet; however, they produced very high amounts when on fresh peppers. Together, this information will be useful in designing better attractant lures for pepper weevils.

  12. The complete mitochondrial genome of Epicauta chinensis (Coleoptera: Meloidae) and phylogenetic analysis among Coleopteran insects.

    Science.gov (United States)

    Du, Chao; He, Shilin; Song, Xuhao; Liao, Qi; Zhang, Xiuyue; Yue, Bisong

    2016-03-10

    The blister beetle is an important resource insect due to its defensive substance cantharidin, which was widely used in pharmacology and plant protection. We determined the complete mitochondrial genome of Epicauta chinensis Laporte (Coleoptera: Tenebrionoidae: Meloidae). The circular genome is 15,717 bp long, encoding 13 protein-coding genes (PCGs), two ribosomal RNAs and 22 tRNAs and containing a A+T-rich region with gene arrangement identical to other Coleopteran species. Twelve PCGs start with typical ATN codon, while ATP8 gene initiate with GTT for first report in Insecta. All PCGs terminate with conventional stop codon TAA or TAG. All tRNAs in E. chinensis are predicted to fold into typical cloverleaf secondary structure, except tRNA-Ser(AGN), in which the dihydrouracil arm (DHU arm) could not form stable stem-loop structure. The secondary structure of lrRNA and srRNA comprises 48 helices and 32 helices respectively. The 1101 bp A+T-rich region contains a 15 bp poly-T stretch and microsatellite-like repeats rather than large tandem repetitive sequences. Phylogenetic analysis, based on 13 PCGs of 45 Coleopteran species, show that E. chinensis grouped with Tenebrionidae species. It also support the topology of (((Chrysomelidae+Curculionoidea)+(Cucujoidea+Cleroidea))+Tenebrionoidea) within Cucujiformia. PMID:26707213

  13. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae

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    M.M. Kiradoo

    2011-03-01

    Full Text Available The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insecticides, there is an utmost need to search for some alternate methods for protection against the damage caused by insects. The leaves of the select plants viz., Ocimum basilicum, O. sanctum and Mentha spicata were employed in the form of various formulations and mortality of the pest insect was assessed. A significant increase in mortality of C. chinensis was observed in all the experimental sets as compared to normal and control sets during the present study. When comparisons were made on the basis of ANOVA to compare the effect of the three plants on mortality, it was found that the treatments of O. basilicum were superior over M. spicata and O. sanctum. The findings overall suggest that all the three plants screened have a potential to be used against the pest C. chinensis and can be employed as an alternate to chemical insecticides in household and storehouses to minimize the infestation and damage caused by the bruchid.

  14. Preliminary Diversity Of Dung Beetles (Coleoptera: Scarabaeinae Attracting By Human Dung (Calvario, Meta

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    Ruth Mariela Castillo Morales

    2011-12-01

    Full Text Available In the Private Reserve, Refugio del Oso de Anteojos (El Calvario-Meta, the diversity of dung beetles was studied (Coleoptera: Scarabaeinae, grouped around different types of vegetation (Montane Rain Forest, Rainforest Premontane, Lower Montane Rain Forest, by using pitfall traps baited with human dung. During the samplings carried out in the rainy station on 2008, were captured a total of 177 individuals belonging to 9 species. The tribe Canthonini was the most representative with 87.5%, of the described species, followed by Aechini 10.8%, and Onthophagini 1.7%. The dominant species was Canthon lituratus in the three vegetation units: Lower montane rain forest was the richest (9 species and abundance (86 individuals, compared with other environments sampled. By calculating the complementarity index, shows a similar species composition in the three habitats sampled, possibly, related to the floristic composition presented in each one of them.

  15. Characterization of the complete mitochondrial genome of the firefly, Luciola substriata (Coleoptera: Lampyridae).

    Science.gov (United States)

    Mu, Feng-Juan; Ao, Liang; Zhao, Hua-Bin; Wang, Kai

    2016-09-01

    The firefly, Luciola substriata (Coleoptera: Lampyridae), is an aquatic firefly species, whose larvae inhabit ponds or lakes. Here we present the complete mitochondrial (mt) genome of the firefly (GenBank accession number KP313820) and provide its annotation. This circular genome is 16,248 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a non-coding AT-rich region. Similar to other firefly species, the base composition of this mitochondrial genome is also biased toward A and T (44.09% A, 34.00% T, 12.89% C, and 9.01% G). All 13 protein-coding genes start with a typical mitochondrial start codon, and terminate with a usual stop codon TAA, or TAG or a single T. The non-coding AT-rich region (1636 bp in length) include one (A)20, and two (T)15 tandem repeats, and one (AAT)5 element. This mitochondrial genome sequence will promote a better understanding for firefly evolution in the future. PMID:25714154

  16. Identification of candidate chemosensory genes in the antennal transcriptome of Tenebrio molitor (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Liu, Su; Rao, Xiang-Jun; Li, Mao-Ye; Feng, Ming-Feng; He, Meng-Zhu; Li, Shi-Guang

    2015-03-01

    We present the first antennal transcriptome sequencing information for the yellow mealworm beetle, Tenebrio molitor (Coleoptera: Tenebrionidae). Analysis of the transcriptome dataset obtained 52,216,616 clean reads, from which 35,363 unigenes were assembled. Of these, 18,820 unigenes showed significant similarity (E-value SNMP) genes. BLASTX best hit results indicated that these chemosensory genes were most identical to their respective orthologs from Tribolium castaneum. Phylogenetic analyses also revealed that the T. molitor OBPs and CSPs are closely related to those of T. castaneum. Real-time quantitative PCR assays showed that eight TmolOBP genes were antennae-specific. Of these, TmolOBP5, TmolOBP7 and TmolOBP16 were found to be predominantly expressed in male antennae, while TmolOBP17 was expressed mainly in the legs of males. Several other genes were identified that were neither tissue-specific nor sex-specific. These results establish a firm foundation for future studies of the chemosensory genes in T. molitor. PMID:25665775

  17. Observations on the Cave-Associated Beetles (Coleoptera of Nova Scotia, Canada

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    Moseley M.

    2009-07-01

    Full Text Available The cave-associated invertebrates of Nova Scotia constitute a fauna at a very early stage of post-glacial recolonization. TheColeoptera are characterized by low species diversity. A staphylinid Quedius spelaeus spelaeus, a predator, is the only regularlyencountered beetle. Ten other terrestrial species registered from cave environments in the province are collected infrequently. Theyinclude three other rove-beetles: Brathinus nitidus, Gennadota canadensis and Atheta annexa. The latter two together with Catopsgratiosus (Leiodidae constitute a small group of cave-associated beetles found in decompositional situations. Quedius s. spelaeusand a small suite of other guanophiles live in accumulations of porcupine dung: Agolinus leopardus (Scarabaeidae, Corticariaserrata (Latrididae, and Acrotrichis castanea (Ptilidae. Two adventive weevils Otiorhynchus ligneus and Barypeithes pellucidus(Curculionidae collected in shallow cave passages are seasonal transients; Dermestes lardarius (Dermestidae, recorded fromone cave, was probably an accidental (stray. Five of the terrestrial beetles are adventive Palaearctic species. Aquatic beetles arecollected infrequently. Four taxa have been recorded: Agabus larsoni (Dytiscidae may be habitual in regional caves; another Agabussp. (probably semivittatus, Dytiscus sp. (Dytiscidae, and Crenitis digesta (Hydrophilidae are accidentals. The distribution andecology of recorded species are discussed, and attention is drawn to the association of beetles found in a Nova Scotia “ice cave”.

  18. Population dynamics and spatial distribution of Abaris basistriata Chaudoir, 1873 (Coleoptera: Carabidae

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    Ivan Carlos Fernandes Martins

    2016-02-01

    Full Text Available ABSTRACT Abaris basistriata, a beetle species dominant in agroecosystems and natural habitats, may benefit from the establishment of nearby refuge areas or crop field centers. To confirm this hypothesis, we analyzed the spatial distribution of the species and verified the population dynamics of this predator in a soybean/corn rotation crop and a central refuge area. The 1-ha experimental area was divided in half by a range of herbaceous plants (2 m in width and 80 m in length. Beetle samples were collected using pitfall traps every fortnight during the in-season and every month during the off-season (a total of 27 sampling occurrences. Population fluctuation was analyzed by correlating the total number of specimens with plant phenology. We used multiple regression analysis with variable (stepwise selection to examine the influence of meteorological factors on species occurrence. To determine the spatial distribution, data were analyzed using dispersion indices and probabilistic models based on the Coleoptera frequency distribution. Distribution visualization was assessed using a linear interpolation map. A total of 143 A. basistriata specimens were collected, with 83 from the soybean/corn area and 60 from the refuge area. Periods of large population size occurred during a season with high rainfall and high maximum and minimum temperatures. On the basis of the spatial distribution analysis of A. basistriata, it is likely that the beetles occur in an aggregate form, preferably in the refuge area.

  19. Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil

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    Andreia Mauruto Chernaki-Leffer

    2011-03-01

    Full Text Available Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer, is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvos. Larvae were fed rabbit feed wetted with a triflumuron-water solution. Concentration-mortality regressions were estimated using Probit analysis and resistance ratios were calculated based on the susceptible population. Among the field populations evaluated, cypermethrin LC50 values for adults, ranged from 68.1 to 6,263 ng (AI/cm². LC50 values for adults challenged with dichlorvos ranged from 10.3 to 1,385 ng (AI/cm². One population from Pato Branco showed reduced susceptibility to triflumuron (LC50 = 272 µg (AI/ml of solution when compared to the most susceptible population (LC50 = 109.8 µg (AI/ml. Application of cypermethrin and dichlorvos analogues should be managed with caution to minimize insecticide resistance problems.

  20. Pitfall Traps and Mini-Winkler Extractor as Complementary Methods to Sample Soil Coleoptera.

    Science.gov (United States)

    Carneiro, A C; Batistella, D A; Battirola, L D; Marques, M I

    2016-02-01

    We compared abundance, species richness, and capture efficiency with pitfall traps and mini-Winkler extractors to examine their use as complementary methods for sampling soil Coleoptera during dry (2010) and high water seasons (2011) in three areas, including inundated and non-inundated regions, in the Pantanal of Poconé, Mato Grosso, Brazil. We paired treatments with two 10 × 10 m plots in inundated and non-inundated locations that were repeated three times in each location for a total of 18 plots. In each plot, we used nine pitfall traps and collected 2 m(2) of leaf litter and surface soil samples with mini-Winkler extractors. We collected a total of 4260 adult beetles comprising 36 families, 113 genera, and 505 species. Most were caught in pitfalls (69%) and the remainder in the mini-Winkler extractors (31%). Each method provided distinct information about the beetle community: 252 species were captured only in pitfall traps, 147 using only the mini-Winkler extractors, and these methods shared another 106 species. Pitfall and mini-Winkler contribute in different ways for the sampling of the soil beetle community, and so they should be considered complementary for a more thorough assessment of community diversity. PMID:26493175

  1. Oil pipeline corridor through an intact forest alters ground beetle (Coleoptera: Carabidae) assemblages in southeastern Ohio.

    Science.gov (United States)

    Silverman, Bareena; Horn, David J; Purrington, Foster F; Gandhi, Kamal J K

    2008-06-01

    Litter-dwelling ground beetle (Coleoptera: Carabidae) assemblages were monitored 1 yr after the construction of a corridor for installation of an oil pipeline along a xeric ridge-top forest in southeastern Ohio. After the creation of the corridor, three distinct habitats were evident in these sites: open corridor, ecotone areas around the corridor, and undisturbed forest interior. Carabidae were collected using directional pitfall traps that were placed parallel and perpendicular to the corridor in each of the three habitats. Results indicate that more carabids were present in the ecotone than in the other two habitats. Carabid diversity as estimated by rarefaction was highest in the corridor followed by ecotone and forest interior. Generalist and forest specialists such as Synuchus impunctatus (Say), Carabus goryi Dejean, and Pterostichus trinarius (Casey) were present in greater numbers in the forest interior and ecotone assemblages. In contrast, open-habitat specialists such as Harpalus pensylvanicus (DeGeer) and Selenophorus opalinus (LeConte) were present in greater numbers in the corridor assemblages. Carabid assemblages of the corridor were distinct from those of the ecotone and forest interior, whereas the latter two habitats had very similar assemblages. The successional pathway of the corridor carabid assemblage will therefore be likely different from that of the forest interior and ecotone. Overall, results indicate that construction of the oil pipeline corridor had significant short-term effects on the carabid numbers, diversity, and species composition because of ensuing habitat changes and fragmentation of the forest. PMID:18559178

  2. Stenusine, an antimicrobial agent in the rove beetle genus Stenus (Coleoptera, Staphylinidae)

    Science.gov (United States)

    Lusebrink, Inka; Dettner, Konrad; Seifert, Karlheinz

    2008-08-01

    Stenusine is well known as the alkaloid, discharged by the rove beetle, genus Stenus Latreille (Coleoptera, Staphylinidae). The Stenus beetles employ the alkaloid as an escape mechanism when on water surfaces. In the case of danger, they lower their abdomen and emit stenusine from their pygidial glands. Stenusine shows a low surface tension and therefore a high spreading pressure; these properties propel the beetle quickly over the water. Many Steninae do not live in habitats with open waters, but in detritus, leaf litter, mosses, etc. This raises the possibility that stenusine might also have another function, e.g., as antibiotic or fungicide. Stenus beetles show an intense grooming behaviour. With gas chromatography mass spectrometry analyses we could prove that they cover themselves with their secretion. To tests its antimicrobial properties we conducted agar diffusion tests with stenusine and norstenusine, another substance that is abundant in most Stenus species. Both compounds have an antimicrobial effect on entomopathogenic bacteria and fungi. Stenusine not only allows for an extraordinary method of locomotion on water surfaces, it also protects the Steninae from being infested with microorganisms.

  3. Demographic comparison and population projection of Rhynchophorus ferrugineus (Coleoptera: Curculionidae) reared on sugarcane at different temperatures.

    Science.gov (United States)

    Peng, Lu; Miao, Yunxin; Hou, Youming

    2016-01-01

    Understanding how temperature affects fitness is important for conservation and pest management, especially in the era of global climate change. Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae) is a worldwide pest of many economically important crops. Although much is known about this pest's life cycle, its adaptability to different temperatures is not fully understood. Here, we used age- and stage-specific life tables to investigate the effects of temperature on fitness-related traits and demographic parameters of R. ferrugineus under eight constant temperature regimens in the laboratory. The growth potential of these populations was also evaluated. The greatest longevity for males and females was 158.0 d at 24 °C and 144.5 d at 21 °C, respectively, but mean total fecundity was the highest at 27 °C. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R0) increased initially at low temperatures and then decreased. All metrics reached a maximum at 27 °C and a minimum at 36 °C. Mean generation times (T ) decreased across the temperature range with a minimum at 36 °C. Our results indicate that the optimum temperature for growth of R. ferrugineus was approximately 27 °C. Our work will be of value for developing strategies for control management of this pest species. PMID:27545594

  4. Notas e novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae

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    Ubirajara R. Martins

    2007-01-01

    Full Text Available Novas espécies descritas do Brasil: Hypsioma carioca sp. nov. (Rio de Janeiro e Hesychotypa maraba sp. nov. (Pará; do Equador: Sternycha ecuatoriana sp. nov. (Pichincha; da Bolívia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. e Alexera secunda sp. nov. Transfere-se Hesycha strandi (Breuning, 1943 para o gênero Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 é considerada sinônima de H. miniata Thomson, 1868. Novo registro (Trinidad e figura são dados para Trachysomus surdus.Notes and new species of Onciderini (Coleoptera, Cerambycidae, Lamiinae. New species described from Brazil: Hypsioma carioca sp. nov. (Rio de Janeiro and Hesychotypa maraba sp. nov. (Pará; from Ecuador: Sternycha ecuatoriana sp. nov. (Pichincha; from Bolivia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. and Alexera secunda sp. nov. Hesycha strandi (Breuning, 1943 is transferred to the genus Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 is considered a synonym of H. miniata Thomson, 1868. New record (Trinidad and figure for Trachysomus surdus Dillon & Dillon, 1946 are given.

  5. Stenusine, an antimicrobial agent in the rove beetle genus Stenus (Coleoptera, Staphylinidae).

    Science.gov (United States)

    Lusebrink, Inka; Dettner, Konrad; Seifert, Karlheinz

    2008-08-01

    Stenusine is well known as the alkaloid, discharged by the rove beetle, genus Stenus Latreille (Coleoptera, Staphylinidae). The Stenus beetles employ the alkaloid as an escape mechanism when on water surfaces. In the case of danger, they lower their abdomen and emit stenusine from their pygidial glands. Stenusine shows a low surface tension and therefore a high spreading pressure; these properties propel the beetle quickly over the water. Many Steninae do not live in habitats with open waters, but in detritus, leaf litter, mosses, etc. This raises the possibility that stenusine might also have another function, e.g., as antibiotic or fungicide. Stenus beetles show an intense grooming behaviour. With gas chromatography-mass spectrometry analyses we could prove that they cover themselves with their secretion. To tests its antimicrobial properties we conducted agar diffusion tests with stenusine and norstenusine, another substance that is abundant in most Stenus species. Both compounds have an antimicrobial effect on entomopathogenic bacteria and fungi. Stenusine not only allows for an extraordinary method of locomotion on water surfaces, it also protects the Steninae from being infested with microorganisms. PMID:18392795

  6. A multilayer micromechanical model of the cuticle of Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae).

    Science.gov (United States)

    Andrew Jansen, M; Singh, Sudhanshu S; Chawla, Nikhilesh; Franz, Nico M

    2016-08-01

    Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae), is a weevil species common throughout the southwestern United States that uses its rostrum - a very slender, curved, beak-like projection of the head - to excavate tunnels in plant organs (such as acorns) for egg laying (oviposition). Once the apical portion of the rostrum has been inserted into the preferred substrate for oviposition, the female begins rotating around the perimeter of the hole, elevating her head by extending the fore-legs, and rotating the head in place in a drilling motion. This action causes significant elastic deformation of the rostrum, which will bend until it becomes completely straight. To better understand the mechanical behavior of the cuticle as it undergoes deformation during the preparation of oviposition sites, we develop a comprehensive micro/macro model of the micromechanical structure and properties of the cuticle, spanning across all cuticular regions, and reliably mirroring the resultant macroscale properties of the cuticle. Our modeling approach relies on the use of multi-scale, hierarchical biomaterial representation, and employs various micromechanical schemata - e.g., Mori-Tanaka, effective field, and Maxwell - to calculate the homogenized properties of representative volume elements at each level in the hierarchy. We describe the configuration and behavior of this model in detail, and discuss the theoretical implications and limitations of this approach with emphasis on future biomechanical and comparative evolutionary research. Our detailed account of this approach can thereby serve as a methodological template for exploring the biomechanical behavior of new insect structures. PMID:27189867

  7. Transcriptomic Analysis of Eucryptorrhynchus chinensis (Coleoptera: Curculionidae) Using 454 Pyrosequencing Technology

    Science.gov (United States)

    Liu, Zhen-Kai; Wen, Jun-Bao

    2016-01-01

    Eucryptorrhynchus chinensis Olivier (Coleoptera: Curculionidae) is one of the most important pests of Ailanthus altissima; however, so far, no studies on the genome or transcriptome of E. chinensis have been reported. Using the Roche 454 FLX Titanium platform, an RNA pool obtained from E. chinensis eggs, larva, pupae, and adults was sequenced and assembled de novo to achieve maximum diversity of sampled transcripts. We obtained 1,441,137 (∼518 Mb) raw reads with an average length of 360 bp. After trimming, 89% qualified reads were produced and assembled into 35,509 isotigs with an average length of 440 bp, N50 of 1,048 bp, and 111,643 singletons. We generated 87,894 unigenes following a cluster analysis of the isotigs and singletons, and then functionally annotated the unigenes with gene descriptions. We obtained 23,363 GO assignments, and 12,724 unigenes were assigned to KOG. Based on these annotations, 294 biochemical pathways involved in growth, reproduction, and stress or immune responses were predicted. A total of 659,026 single nucleotide variants and 6,112 simple sequence repeats were detected. Our data provide comprehensive information on the sequence and possible functions of E. chinensis transcripts.

  8. Endemic epigean Tenebrionids (Coleoptera: Tenebrionidae) from the Andean Region: exploring the patagonian-diversification hypothesis.

    Science.gov (United States)

    Carrara, Rodolfo; Flores, Gustavo E

    2015-01-01

    Tenebrionidae is a diverse insect family of Coleoptera that shows high levels of endemicity in epigean species. For the Andean region, which is divided into three subregions: Central Chilean, Subantarctic and Patagonian, it has been hypothesized that epigean tenebrionids have diversified in the Patagonian subregion and subsequently, they dispersed to Subantarctic and Central Chilean subregions. In this work, based on information obtained from museum collections and scientific studies, we presented the first list of endemic epigean tenebrionids from the Andean region with their taxonomic arrangement and geographic distribution. Moreover, we used these data to explore the veracity of the Patagonian-diversification hypothesis. A total of 416 species grouped into six subfamilies, 17 tribes and 41 genera were identified as endemic to the Andean region. Considering the spatial distribution it was observed that subfamilies, tribes, genera and species were unequally distributed across subregions. Results did not support the Patagonian-diversification hypothesis; to the contrary, they were more concordant with processes of isolation among subregions that have promoted speciation by interrupting gene flow among populations, resulting in endemism because species can not expand their range sizes. Finally, we discuss the implications of our findings to be considered in biodiversity conservation, because endemic species, by their high extinction risk, are primary targets in conservation strategies. PMID:26623788

  9. Pathology of Yersinia entomophaga MH96 towards Costelytra zealandica (Coleoptera; Scarabaeidae) larvae.

    Science.gov (United States)

    Hurst, Mark Robin Holmes; van Koten, Chikako; Jackson, Trevor Anthony

    2014-01-01

    The bacterium Yersinia entomophaga was isolated from larvae of the New Zealand grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), found in soil. Following ingestion of a lethal dose of bacteria, larvae of C. zealandica reduced feeding activity and movement. After approximately 4h infected larvae convulsed and regurgitated dark digestive fluid and expelled frass pellets leaving the midgut empty and the larva amber in appearance. In the initial stages of infection, ingested bacteria were mostly contained within the peritrophic membrane and expelled with the gut fluid or transferred into the hind gut. While few Y. entomophaga were associated with the midgut epithelial cells, by 24h cells were swelling and bursting with vesicles being expelled into the midgut lumen. By 48h, bacteria had entered the haemocoel and the midgut cells had further deteriorated. After 72h, the cellular remnants were totally detached from the basal membrane the infected insects were filled with bacteria and moribund or dead with septicaemia. Mortality was directly proportional to dose and time after infection. By applying a range of doses, the LD50 was determined as 2.9×10(4)Y. entomophaga per C. zealandica larva, with an LT50 of 2.94days for doses of>1×10(5) per larva. Ingestion of low doses of bacteria did not inhibit feeding activity but led more slowly to death. By time of death, Y. entomophaga had multiplied, approximately 500 fold, in the cadavers of the infected larvae. PMID:24291403

  10. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases.

    Science.gov (United States)

    Downey, Danielle; Chun, Stacey; Follett, Peter

    2015-06-01

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. PMID:26470205

  11. Ambrosia Beetle (Coleoptera: Scolytidae) Species, Flight, and Attack on Living Eastern Cottonwood Trees.

    Energy Technology Data Exchange (ETDEWEB)

    Coyle, D R; D.C. Booth: M.S. Wallace

    2005-12-01

    ABSTRACT In spring 2002, ambrosia beetles (Coleoptera: Scolytidae) infested an intensively managed 22-ha tree plantation on the upper coastal plain of South Carolina. Nearly 3,500 scolytids representing 28 species were captured in ethanol-baited traps from 18 June 2002 to 18 April 2004. More than 88% of total captures were exotic species. Five species [Dryoxylon onoharaensum (Murayama), Euwallacea validus (Eichhoff), Pseudopityophthorus minutissimus (Zimmermann), Xyleborus atratus Eichhoff, and Xyleborus impressus Eichhoff]) were collected in South Carolina for the first time. Of four tree species in the plantation, eastern cottonwood, Populus deltoides Bartram, was the only one attacked, with nearly 40% of the trees sustaining ambrosia beetle damage. Clone ST66 sustained more damage than clone S7C15. ST66 trees receiving fertilization were attacked more frequently than trees receiving irrigation, irrigation_fertilization, or controls, although the number of S7C15 trees attacked did not differ among treatments. The study location is near major shipping ports; our results demonstrate the necessity for intensive monitoring programs to determine the arrival, spread, ecology, and impact of exotic scolytids.

  12. Evolution of subterranean diving beetles (Coleoptera: Dytiscidae: Hydroporini, Bidessini) in the arid zone of Australia.

    Science.gov (United States)

    Leys, Remko; Watts, Chris H S; Cooper, Steve J B; Humphreys, William F

    2003-12-01

    Calcrete aquifers in arid inland Australia have recently been found to contain the world's most diverse assemblage of subterranean diving beetles (Coleoptera: Dytiscidae). In this study we test whether the adaptive shift hypothesis (ASH) or the climatic relict hypothesis (CRH) is the most likely mode of evolution for the Australian subterranean diving beetles by using a phylogeny based on two sequenced fragments of mitochondrial genes (CO1 and 16S-tRNA-ND1) and linearized using a relaxed molecular clock method. Most individual calcrete aquifers contain an assemblage of diving beetle species of distantly related lineages and/or a single pair of sister species that significantly differ in size and morphology. Evolutionary transitions from surface to subterranean life took place in a relatively small time frame between nine and four million years ago. Most of the variation in divergence times of the sympatric sister species is explained by the variation in latitude of the localities, which correlates with the onset of aridity from the north to the south and with an aridity maximum in the Early Pliocene (five mya). We conclude that individual calcrete aquifers were colonized by several distantly related diving beetle lineages. Several lines of evidence from molecular clock analyses support the CRH, indicating that all evolutionary transitions took place during the Late Miocene and Early Pliocene as a result of aridification. PMID:14761060

  13. Culturable bacterial microbiota of Plagiodera versicolora (L.) (Coleoptera: Chrysomelidae) and virulence of the isolated strains.

    Science.gov (United States)

    Demirci, Meryem; Sevim, Elif; Demir, İsmail; Sevim, Ali

    2013-05-01

    Plagiodera versicolora (Laicharting, 1781) (Coleoptera: Chrysomelidae) is an important forest pest which damages many trees such as willow, poplar, and hazelnut. In order to find new microbes that can be utilized as a possible microbial control agent against this pest, we investigated the culturable bacterial flora of it and tested the isolated bacteria against P. versicolora larvae and adults. We were able to isolate nine bacteria from larvae and adults. The isolates were characterized using a combination of morphological, biochemical, and physiological methods. Additionally, we sequenced the partial sequence of the 16S rRNA gene to verify conventional identification results. Based on characterization studies, the isolates were identified as Staphylococcus sp. Pv1, Rahnella sp. Pv2, Rahnella sp. Pv3, Rahnella sp. Pv4, Rahnella sp. Pv5, Pantoea agglomerans Pv6, Staphylococcus sp. Pv7, Micrococcus luteus Pv8, and Rahnella sp. Pv9. The highest insecticidal activity against larvae and adults was obtained from M. luteus Pv8 with 50 and 40 % mortalities within 10 days after treatment, respectively. Extracellular enzyme activity of the bacterial isolates such as amylase, proteinase, lipase, cellulose, and chitinase was also determined. Consequently, our results show that M. luteus Pv8 might be a good candidate as a possible microbial control agent against P. versicolora and were discussed with respect to biocontrol potential of the bacterial isolates. PMID:23054688

  14. Morfología del tracto digestivo y sistema reproductor femenino de Compsus sp. (Coleoptera: Curculionidae Morphology of the digestive tract and female reproductive system of Compsus sp. (Coleoptera: Curculionidae

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    JOSÉ DAVID RUBIO G

    2007-12-01

    Full Text Available Se realizó el estudio morfológico del tracto digestivo y sistema reproductor femenino del picudo de los cítricos, Compsus sp. (Coleoptera: Curculionidae. El tracto digestivo presentó un arreglo típico descrito para otros insectos. Se encontraron diferencias morfológicas, sin embargo, en el buche en donde se observan unas estructuras espinosas. A nivel del mesenterón, se observó que el ventrículo anterior se encuentra dividido en sacos de tamaño medio y en el ventrículo posterior se proyectan numerosos ciegos gástricos. El sistema reproductor de la hembra mostró un arreglo estructural común a especímenes de la familia Curculionidae.A morphological examination was conducted on the digestive tract and the female reproductive system of the citrus weevil, Compsus sp. (Coleoptera: Curculionidae. The digestive tract of Compsus sp. showed a typical arrangement as described for other insects. Morphological differences, however, were found in the crop of the foregut, which exhibited several spine-like structures. At the midgut level, the anterior ventricle was divided into medium-size sacs and numerous gastric caecae projected into the posterior ventricle. The female reproductive system showed a structural arrangement common to individuals of the family Curculionidae.

  15. Cowpea Vicilins: Fractionation of Urea Denatured Sub-Units and Effects on Callosobruchus maculatus F. (Coleoptera: Bruchidae Development

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    Antônio Chagas Mota

    2002-03-01

    Full Text Available Vicilins (7S storage globulins isolated from cowpea (Vigna unguiculata L. seeds which were susceptible (S and resistant (R to the cowpea weevil (Callosobruchus maculatus F., Coleoptera: Bruchidae were denatured by urea and fractionated by ion-exchange chromatography. Isolated fractions were incorporated in artificial seeds for assessment of their toxicity to C. maculatus. The most acidic fractions of both susceptible (CE-31 cultivar and resistant (IT81D-1045 line seeds were shown to affect development and survival of the bruchid. Results indicated that vicilin polypeptides of toxic nature were expressed in both types of storage globulins although at different levels.Vicilinas (globulinas de reserva 7S isoladas de sementes de feijão-de-corda (Vigna unguiculata L., susceptíveis (S e resistentes (R ao caruncho/gorgulho (Callosobruchus maculatus F., Coleoptera: Bruchidae foram desnaturadas por uréia e fracionadas por cromatografia de troca iônica. As frações isoladas foram incorporadas em sementes artificiais para avaliação de sua toxicidade a C. maculatus. As fracões mais ácidas de ambas vicilinas afetaram o desenvolvimento e a sobrevivência do bruquídeo. Sugerimos que polipeptídeos de vicilinas de natureza tóxica são expressos em ambos tipos de globulinas de reserva, embora em níveis diferentes.

  16. Typhlocharis vicariantes del Estrecho de Gibraltar. I: Typhlocharis armata Coiffait, 1969 (Coleoptera, Caraboidea, Trechidae

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    Pérez González, S.

    2010-12-01

    Full Text Available This first part about the vicariant Typhlocharis from Gibraltar Strait is dedicated to Typhlocharis armata Coiffait, 1969; a second paper will cover T. silvanoides Dieck, 1869. The studied material was obtained in four field campaigns carried out in the south of Spain and north of Morocco between 1997 and 2001 (Zaballos & Banda, 2000; Zaballos, 2005. In these campaigns abundant specimens of Typhlocharis (Coleoptera, Caraboidea, Trechidae were collected in different localities, the majority of them belonging to the species T. armata Coiffait, 1969. Their study and the comparison with the type series of this species allowed its complete redescription and clarification of its status within the genus. Contrary to what has been considered traditionally, the marginal umbilicate series of elytra in T. armata is composed of three, instead of four posterior setae. The taxonomical affinities of this species are established, the morphological weight of the umbilicate group of elytral setae as a lineage and species group defining character is discussed, and the theory of Jeanne (1973 about the relation between shortening of elytra and umbilicate series reduction with a decrease in body size is revised. The dispersal abilities are evaluated and a biogeographical explanation for the new distribution of the species is proposed.Esta primera parte sobre las especies de Typhlocharis vicariantes del Estrecho de Gibraltar está dedicada a Typhlocharis armata Coiffait, 1969; mientras que en un segundo artículo se tratará T. silvanoides Dieck, 1869. El material estudiado procede de cuatro campañas entomológicas realizadas en el sur de España y en el norte de Marruecos entre 1997 y 2001 (Zaballos & Banda, 2000; Zaballos, 2005. En estas campañas se recolectaron, en localidades diferentes, abundantes ejemplares de Typhlocharis (Coleoptera, Caraboidea, Trechidae, la mayoría de los cuales han resultado pertenecer a la especie T. armata Coiffait, 1969. Su estudio y

  17. Morphological and genetic variation in Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae, Cicindelinae: implications for conservation

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    Serrano, A.

    2003-12-01

    Full Text Available The establishment of taxonomic and geographical boundaries is a common problem when analysing clinal distributions. This is of particular concern when the assessment of intraspecific groupings is required for conservation management. The tiger beetle Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae is a typical case in which two recognised subspecies are distributed in a clinal latitudinal fashion in the dune systems along the Atlantic coast of Portugal. This habitat is increasingly under threat, and conservation measures are needed. We investigated the validity of the two named subspecies, based on a re-analysis of elytral and genitalic measurements using multivariate analysis. We also analysed variation in mitochondrial Cytochrome Oxidase I gene for a total of six populations along the cline. Multivariate analysis supported the idea of a morphological cline and revealed a clear distinction of the southernmost population and also some degree of distinctiveness of the most northern populations, partially supporting the recognised subspecific ranking. The mtDNA analysis identified two main groups corresponding to northern and southern populations. Both sets of markers showed that variation within the C. lusitanica assemblage is complex, with the boundaries between morphological and mtDNA groups not in agreement. However, populations at either end of the distributional range are clearly distinct from each other, and should be considered as provisional units for conservation programmes.El reconocimiento de límites taxonómicos y geográficos de la variabilidad observada es un problema habitual cuando se analizan distribuciones clinales. Esto es particularmente problemático cuando se requiere la determinación de agrupamientos intraespecíficos para tomar medidas de conservación. El cicindélido Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae constituye un caso típico en el que dos subespecies reconocidas se hayan distribuidas a

  18. Observations on sex ratio and behavior of males in Xyleborinus saxesenii Ratzeburg (Scolytinae, Coleoptera

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    Peter Biedermann

    2010-09-01

    Full Text Available Strongly female-biased sex ratios are typical for the fungal feeding haplodiploid Xyleborini (Scolytinae, Coleoptera, and are a result of inbreeding and local mate competition (LMC. These ambrosia beetles are hardly ever found outside of trees, and thus male frequency and behavior have not been addressed in any empirical studies to date. In fact, for most species the males remain undescribed. Data on sex ratios and male behavior could, however, provide important insights into the Xyleborini’s mating system and the evolution of inbreeding and LMC in general. In this study, I used in vitro rearing methods to obtain the first observational data on sex ratio, male production, male and female dispersal, and mating behavior in a xyleborine ambrosia beetle. Females of Xyleborinus saxesenii Ratzeburg produced between 0 and 3 sons per brood, and the absence of males was relatively independent of the number of daughters to be fertilized and the maternal brood sex ratio. Both conformed to a strict LMC strategy with a relatively precise and constant number of males. If males were present they eclosed just before the first females dispersed, and stayed in the gallery until all female offspring had matured. They constantly wandered through the gallery system, presumably in search of unfertilized females, and attempted to mate with larvae, other males, and females of all ages. Copulations, however, only occurred with immature females. From galleries with males, nearly all females dispersed fertilized. Only a few left the natal gallery without being fertilized, and subsequently went on to produce large and solely male broods. If broods were male-less, dispersing females always failed to found new galleries.

  19. Diel Patterns of Colaspis brunnea and Colaspis crinicornis (Coleoptera: Chrysomelidae) in Southeastern Nebraska.

    Science.gov (United States)

    Miwa, Kentaro; Meinke, Lance J

    2015-12-01

    A field study was conducted to increase our understanding of diel activity patterns of Colaspis brunnea (F.) and Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) in key crop habitats. Within 24-h periods, C. brunnea was sampled in clover fields (primarily red clover, Trifolium pretense (L.), with some sweet clover, Melilotus officinalis (L.) Pallas, and downy brome, Bromus tectorum (L.)) and soybean, Glycine max (L.) Merrill, fields, using a sweep-net, while whole-plant-count sampling was used to monitor C. crinicornis densities in field corn, Zea mays (L.). Sweep-net captures of C. brunnea were significantly greater at night than during the day, suggesting possible vertical movement within the canopy during a 24-h period. Colaspis crinicornis densities on corn plants were fairly constant throughout a 24-h period, but beetle activity (e.g., walking, mating) was significantly greater at night than during the day. Results suggest that both Colaspis species may be exhibiting similar increases in activity at night that facilitates movement from more protected to more exposed areas within a habitat. It is unclear what mechanisms drive this diel pattern, but vegetation architecture and associated interactions with environmental conditions may play a role. Sweep-netting in clover or soybean fields and use of whole-plant-counts in cornfields were effective sampling methods for Colaspis adults. However, because activity and behaviors of Colaspis beetles were influenced by time of day in this study, use of a consistent sampling time within a diel period would be recommended for future population studies or integrated pest management decision-making. PMID:26314034

  20. Organization and comparative analysis of the mitochondrial genomes of bioluminescent Elateroidea (Coleoptera: Polyphaga).

    Science.gov (United States)

    Amaral, Danilo T; Mitani, Yasuo; Ohmiya, Yoshihiro; Viviani, Vadim R

    2016-07-25

    Mitochondrial genome organization in the Elateroidea superfamily (Coleoptera), which include the main families of bioluminescent beetles, has been poorly studied and lacking information about Phengodidae family. We sequenced the mitochondrial genomes of Neotropical Lampyridae (Bicellonycha lividipennis), Phengodidae (Brasilocerus sp.2 and Phrixothrix hirtus) and Elateridae (Pyrearinus termitilluminans, Hapsodrilus ignifer and Teslasena femoralis). All species had a typical insect mitochondrial genome except for the following: in the elaterid T. femoralis genome there is a non-coding region between NADH2 and tRNA-Trp; in the phengodids Brasilocerus sp.2 and P. hirtus genomes we did not find the tRNA-Ile and tRNA-Gln. The P. hirtus genome showed a ~1.6kb non-coding region, the rearrangement of tRNA-Tyr, a new tRNA-Leu copy, and several regions with higher AT contents. Phylogenetics analysis using Bayesian and ML models indicated that the Phengodidae+Rhagophthalmidae are closely related to Lampyridae family, and included Drilus flavescens (Drilidae) as an internal clade within Elateridae. This is the first report that compares the mitochondrial genomes organization of the three main families of bioluminescent Elateroidea, including the first Neotropical Lampyridae and Phengodidae. The losses of tRNAs, and translocation and duplication events found in Phengodidae mt genomes, mainly in P. hirtus, may indicate different evolutionary rates in these mitochondrial genomes. The mitophylogenomics analysis indicates the monophyly of the three bioluminescent families and a closer relationship between Lampyridae and Phengodidae/Rhagophthalmidae, in contrast with previous molecular analysis. PMID:27060405

  1. The presence of a mycangium in European Sinodendron cylindricum (Coleoptera: Lucanidae) and the associated yeast symbionts

    Science.gov (United States)

    Tanahashi, Masahiko; Hawes, Colin J.

    2016-01-01

    Part of the exoskeleton of some wood-inhabiting insects is modified to form a mycangium, which is a specialized organ used to convey fungal spores or yeasts to their offspring. Although most stag beetles (Coleoptera: Lucanidae) are known to have female-specific mycangia and associated yeast symbionts, the evolutionary origin of the mycangium in this group remains unresolved. Here, we report the presence of a mycangium and associated yeast symbionts in the European horned stag beetle Sinodendron cylindricum (L.), which belongs to an ancestral clade of the Lucanidae. The mycangium of S. cylindricum is shown to be female-specific and have the same developmental origin as that of other stag beetles. A total of five yeast strains were isolated from adult mycangia and larval gut of S. cylindricum. Of these, we suggest that SICYAM1 is an undescribed yeast with taxonomic novelty, and have identified SICYLG3 as the xylose-fermenting yeast Scheffersomyces insectosa using nuclear ribosomal RNA and ITS sequences. The remaining three yeast strains, SICYAM2, SICYLG1, and SICYLG2, were assigned to the genus Sugiyamaella. Yeast density in the adult mycangium was lower than that of the more evolutionarily advanced stag beetles, the European Lucanus cervus (L.) and Dorcus parallelipipedus (L.), which were also examined in this study. No living yeasts were isolated from the adult guts. However, a third instar larva of S. cylindricum harbored 104–106 living yeasts in each gut region, which suggests that gut yeasts play an important role in these wood-feeding larvae. PMID:27432353

  2. Relationships of abscised cotton fruit to boll weevil (Coleoptera: Curculionidae) feeding, oviposition, and development.

    Science.gov (United States)

    Showler, Allan T

    2008-02-01

    Abscised cotton, Gossypium hirsutum L., fruit in field plots planted at different times were examined to assess adult boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), use of squares and bolls during 2002 and 2003 in the Lower Rio Grande Valley of Texas. Although boll abscission is not necessarily related to infestation, generally more bolls abscised than squares and abundances of fallen bolls were not related to the planting date treatments. During 2003, fallen squares were most abundant in the late-planted treatment. Although large squares (5.5-8-mm-diameter) on the plant are preferred for boll weevil oviposition, diameter of abscised squares is not a reliable measurement because of shrinkage resulting from desiccation and larval feeding. Fallen feeding-punctured squares and bolls were most abundant in late plantings but differences between fallen feeding-punctured squares versus fallen feeding-punctured bolls were found in only one treatment in 2003. During the same year, fallen oviposition-punctured squares were more numerous in the late-planted treatment than in the earlier treatments. Treatment effects were not found on numbers of oviposition-punctured bolls, but fallen oviposition-punctured squares were more common than bolls in the late-planted treatment compared with earlier treatments each year. Dead weevil eggs, larvae, and pupae inside fallen fruit were few and planting date treatment effects were not detected. Living third instars and pupae were more abundant in fallen squares of the late-planted treatment than in the earlier treatments and bolls of all three treatments. This study shows that fallen squares in late-planted cotton contribute more to adult boll weevil populations than bolls, or squares of earlier plantings. PMID:18330118

  3. Influence of trap color and host volatiles on capture of the emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Crook, Damon J; Khrimian, Ashot; Cossé, Allard; Fraser, Ivich; Mastro, Victor C

    2012-04-01

    Field trapping assays were conducted in 2009 and 2010 throughout western Michigan, to evaluate lures for adult emerald ash borer, A. planipennis Fairmaire (Coleoptera: Buprestidae). Several ash tree volatiles were tested on purple prism traps in 2009, and a dark green prism trap in 2010. In 2009, six bark oil distillate lure treatments were tested against manuka oil lures (used in 2008 by USDA APHIS PPQ emerald ash borer cooperative program). Purple traps baited with 80/20 (manuka/phoebe oil) significantly increased beetle catch compared with traps baited with manuka oil alone. In 2010 we monitored emerald ash borer attraction to dark green traps baited with six lure combinations of 80/20 (manuka/phoebe), manuka oil, and (3Z)-hexenol. Traps baited with manuka oil and (3Z)-hexenol caught significantly more male and total count insects than traps baited with manuka oil alone. Traps baited with manuka oil and (3Z)-hexenol did not catch more beetles when compared with traps baited with (3Z)-hexenol alone. When compared with unbaited green traps our results show that (3Z)-hexenol improved male catch significantly in only one of three field experiments using dark green traps. Dark green traps caught a high number of A. planipennis when unbaited while (3Z)-hexenol was seen to have a minimal (nonsignificant) trap catch effect at several different release rates. We hypothesize that the previously reported kairomonal attractancy of (3Z)-hexenol (for males) on light green traps is not as obvious here because of improved male attractancy to the darker green trap. PMID:22606813

  4. Displacement of Tetropium cinnamopterum (Coleoptera: Cerambycidae) by Its Invasive Congener Tetropium fuscum.

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    Dearborn, Kenneth W; Heard, Stephen B; Sweeney, Jon; Pureswaran, Deepa S

    2016-08-01

    We examined the native community of insects interacting with an invasive species, Tetropium fuscum (F.) (Coleoptera: Cerambycidae), in its new range to explore reasons for the invader's relatively slow spread. Tetropium fuscum is a European spruce borer established in Nova Scotia since at least 1990, but it has spread only about 125 km from its site of introduction. We compared the densities of Tetropium spp., their known parasitoids, and the community of wood-boring insects at sites located within the invasion zone in Nova Scotia versus well outside this zone, in New Brunswick, Canada. Using red spruce trees stressed by girdling or felling, we tested whether: 1) T. fuscum had altered the native wood-boring community; 2) T. fuscum displaced a native congener, Tetropium cinnamopterum (Kirby); and 3) parasitism rates of Tetropium spp. differed between the invaded and noninvaded zones. Both Tetropium spp. and their parasitoid wasps emerged exclusively from felled trees as opposed to girdled trees. We found no difference in community diversity inside versus outside the invasion zone. The combined densities of both Tetropium spp. and their overall parasitism rates also did not differ between zones, but T. cinnamopterum density was significantly greater outside the invasion zone, suggesting T. fuscum may displace the native congener where they are sympatric. Our results suggest that the native and invasive Tetropium spp. act as a single functional species in the invasion zone. We speculate that natural control agents (predators, parasitoids, and competitors) might be limiting the rate of spread of T. fuscum. PMID:27247305

  5. Exploring the Leaf Beetle Fauna (Coleoptera: Chrysomelidae of an Ecuadorian Mountain Forest Using DNA Barcoding.

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    Birthe Thormann

    Full Text Available Tropical mountain forests are hotspots of biodiversity hosting a huge but little known diversity of insects that is endangered by habitat destruction and climate change. Therefore, rapid assessment approaches of insect diversity are urgently needed to complement slower traditional taxonomic approaches. We empirically compare different DNA-based species delimitation approaches for a rapid biodiversity assessment of hyperdiverse leaf beetle assemblages along an elevational gradient in southern Ecuador and explore their effect on species richness estimates.Based on a COI barcode data set of 674 leaf beetle specimens (Coleoptera: Chrysomelidae of 266 morphospecies from three sample sites in the Podocarpus National Park, we employed statistical parsimony analysis, distance-based clustering, GMYC- and PTP-modelling to delimit species-like units and compared them to morphology-based (parataxonomic species identifications. The four different approaches for DNA-based species delimitation revealed highly similar numbers of molecular operational taxonomic units (MOTUs (n = 284-289. Estimated total species richness was considerably higher than the sampled amount, 414 for morphospecies (Chao2 and 469-481 for the different MOTU types. Assemblages at different elevational levels (1000 vs. 2000 m had similar species numbers but a very distinct species composition for all delimitation methods. Most species were found only at one elevation while this turnover pattern was even more pronounced for DNA-based delimitation.Given the high congruence of DNA-based delimitation results, probably due to the sampling structure, our study suggests that when applied to species communities on a regionally limited level with high amount of rare species (i.e. ~50% singletons, the choice of species delimitation method can be of minor relevance for assessing species numbers and turnover in tropical insect communities. Therefore, DNA-based species delimitation is confirmed as a

  6. Dung beetle assemblages (Coleoptera, Scarabaeinae in Atlantic forest fragments in southern Brazil

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    Renata C. Campos

    2013-03-01

    Full Text Available Dung beetle assemblages (Coleoptera, Scarabaeinae in Atlantic forest fragments in southern Brazil. The beetles of the subfamily Scarabaeinae are important organisms that participate in the cycle of decomposition, especially in tropical ecosystems. Most species feed on feces (dung or carcasses (carrion and are associated with animals that produce their food resources. Dung beetles are divided into three functional groups: rollers, tunnelers and dwellers. This present work aims to study the diversity of dung beetle communities inhabiting fragments of the Atlantic Forest, with the purpose of describing the ecology of the species in southern Brazil. This study was conducted in the region of Campos Novos, in Santa Catarina, where twenty sites of Atlantic forest fragments were sampled. Samplings of dung beetles were conducted using 200 pitfall traps, of which 100 were baited with human feces and another 100 with carrion. Size and environmental complexity were also measured for each forest fragment. A total of 1,502 dung beetles, belonging to six tribes, 12 genera and 33 species, were collected. Results of the Levin's index of niche breadth indicated that 11 species were categorized as being coprophagous, ten as generalists, and two as necrophagous. Most species are tunnelers (19, nine of rollers and four of dwellers. The great diversity of Scarabaeinae in the region of Campos Novos, including several rare species, adds important data to the Scarabaeinae fauna in the central-western region of Santa Catarina. It may also help choosing priority areas for conservation in the region, where human impact, with large areas of monoculture, increasingly threatens the fragments of Mixed Ombrophilous Forest.

  7. Scarab Beetle (Coleoptera: Scarabaeidae Fauna in Ardabil Province, North West Iran

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    G Mowlavi

    2008-12-01

    Full Text Available Background: Dung beetles of Coleoptera associated to undisturbed cattle droppings in pastures present great diver¬sity and abundance. Dung beetles also play an important role for transmission of some helminthes to human and cat¬tle. This study was made to survey the biodiversity and abundance of these beetles in Ardebil Province, western Iran.Methods: According to the field study all beetles attracted to fresh cow dung in five areas of Ardebil Province in¬cluding Namin, Ardabil, Meshkinshahr, Neer and Sarein were collected and identified. They were collected during summer 2007 from June to September, with general peaks appearing to be correlated with temperature mainly at 11 a.m to 15 p.m. The samples were identified using appropriate systematic key Results: A total of 231 specimens belonging to 9 beetle genera and at least 15 species were identified as Euoniticel¬lus fulvus, Sisyphus schaffaer, Euonthophagus taurus, Copris lunaris, Chironitis pamphilus, Gymnopleurus coriarus, Euonthophagus amyntas, Caccobius schreberi, Onthophagus speculifer, Onthophagus furcatus, Aphodius, lugens, Apho¬dius fimetarius, A. scrutator, Geotrupes spiniger and G. stercorariusThe most abundant and diverse subfamilies were Coprinae, Geotrupinae, and Aphodiinae. Conclusion: We found 15 species of dung beetles occurred in the region. The prevalence of each species is varied depending on location. Some of them play an important role for helminths transmission of veterinary and public health importance. The finding will provide a clue for pasture management as well as public health monitoring and surveillance of the disease transmitted by dung beetles. 

  8. Scarab Beetle (Coleoptera: Scarabaeidae Fauna in Ardabil Province, North West Iran

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    G Mowlavi

    2008-12-01

    Full Text Available "nBackground: Dung beetles of Coleoptera associated to undisturbed cattle droppings in pastures present great diver¬sity and abundance. Dung beetles also play an important role for transmission of some helminthes to human and cat¬tle. This study was made to survey the biodiversity and abundance of these beetles in Ardebil Province, western Iran."nMethods: According to the field study all beetles attracted to fresh cow dung in five areas of Ardebil Province in¬cluding Namin, Ardabil, Meshkinshahr, Neer and Sarein were collected and identified. They were collected during summer 2007 from June to September, with general peaks appearing to be correlated with temperature mainly at 11 a.m to 15 p.m. The samples were identified using appropriate systematic key "nResults: A total of 231 specimens belonging to 9 beetle genera and at least 15 species were identified as Euoniticel¬lus fulvus, Sisyphus schaffaer, Euonthophagus taurus, Copris lunaris, Chironitis pamphilus, Gymnopleurus coriarus, Euonthophagus amyntas, Caccobius schreberi, Onthophagus speculifer, Onthophagus furcatus, Aphodius, lugens, Apho¬dius fimetarius, A. scrutator, Geotrupes spiniger and G. stercorarius"nThe most abundant and diverse subfamilies were Coprinae, Geotrupinae, and Aphodiinae. "nConclusion: We found 15 species of dung beetles occurred in the region. The prevalence of each species is varied depending on location. Some of them play an important role for helminths transmission of veterinary and public health importance. The finding will provide a clue for pasture management as well as public health monitoring and surveillance of the disease transmitted by dung beetles. 

  9. A comparison of trap type and height for capturing cerambycid beetles (Coleoptera).

    Science.gov (United States)

    Graham, Elizabeth E; Poland, Therese M; McCullough, Deborah G; Millar, Jocelyn G

    2012-06-01

    Wood-boring beetles in the family Cerambycidae (Coleoptera) play important roles in many forest ecosystems. However, increasing numbers of invasive cerambycid species are transported to new countries by global commerce and threaten forest health in the United States and worldwide. Our goal was to identify effective detection tools for a broad array of cerambycid species by testing some known cerambycid attractants and a pheromone in different trap designs placed across a range of habitats. We compared numbers and species richness of cerambycid beetles captured with cross-vane panel traps and 12-unit Lindgren multiple-funnel traps, placed either at ground level (1.5 m high) or canopy level (approximately 3-10 m high), at eight sites classified as either residential, industrial, deciduous forest, or conifer forest. We captured 3,723 beetles representing 72 cerambycid species from 10 June to 15 July 2010. Species richness was highest for the subfamilies Cerambycinae and Lamiinae, which accounted for 33 and 46% of all species captured, respectively. Overall, the cross-vane panel traps captured approximately 1.5 times more beetles than funnel traps. Twenty-one species were captured exclusively in traps at one height, either in the canopy or at ground level. More species were captured in hardwood sites (59 species) where a greater diversity of host material was available than in conifer (34 species), residential (41 species), or industrial (49) sites. Low numbers of beetles (n < 5) were recorded for 28 of the beetle species. The number of species captured per week ranged from 49 species on 21 June to 37 species on 12 July. Cross-vane panel traps installed across a vertical gradient should maximize the number of cerambycid species captured. PMID:22812119

  10. Tropical Plant Extracts as Sources of Grain-Protectant Compounds Against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae).

    Science.gov (United States)

    Ribeiro, L P; Vendramim, J D; Andrade, M S; Bicalho, K U; Silva, M F G F; Vieira, P C; Fernandes, J B

    2014-10-01

    The toxicity of organic plant extracts to Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) was assessed for three tropical plant species: branches, leaves, and seeds of Annona montana (Annonaceae), branches of Aristolochia paulistana (Aristolochiaceae), and leaves and branches of Casearia sylvestris (Salicaceae). The screening assay resulted that the extracts of A. montana seeds obtained with hexane (LC50 = 534.75 mg kg(-1) and LT50 = 6.10 days) and with dichloromethane (LC50 = 424.67 mg kg(-1) and LT50 = 5.03 days) were the most promising treatments, followed by the extract prepared from A. montana leaves with hexane (LC50 = 837.70 mg kg(-1) and LT50 = 4.90 days). Moreover, extracts (at 1,500 mg kg(-1)) prepared from C. sylvestris branches with dichloromethane and A. paulistana with hexane caused significant mortality (37% and 41.5%, respectively) beyond sublethal effects on S. zeamais. Therefore, based on the biological assays, extraction yield, and evaluation of the chromatographic profile of the crude extracts by TLC, the hexane extract of A. montana seeds was selected and fractioned using liquid-liquid partitioning. The hydroalcoholic fraction caused mortality of 55.5%, significantly superior to dichloromethane fraction, which caused 35.5% of mortality. Chemical analyses ((1)H NMR, HPLC, and TLC) were performed, and the results showed the presence of alkaloids and acetogenins in both active fractions, which have been associated with bioactivity. Therefore, extracts prepared from A. montana seeds (especially) is an interesting source of new compounds with promising grain-protectant properties. PMID:27193958

  11. Variability in Small Hive Beetle (Coleoptera: Nitidulidae) Reproduction in Laboratory and Field Experiments.

    Science.gov (United States)

    Meikle, William G; Holst, Niels; Cook, Steven C; Patt, Joseph M

    2015-06-01

    Experiments were conducted to examine how several key factors affect population growth of the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae). Laboratory experiments were conducted to examine effects of food quantity and temperature on reproduction of cohorts of young A. tumida adults (1:1 sex ratio) housed in experimental arenas. Daily numbers and total mass of larvae exiting arenas were highly variable within treatment. Either one or two cohorts of larvae were observed exiting the arenas. Food quantity, either 10 g or 20 g, did not significantly affect the number of larvae exiting arenas at 32°C, but did at 28°C; arenas provided 20 g food produced significantly more larvae than arenas provided 10 g. Temperature did not affect the total mass of larvae provided 10 g food, but did affect larval mass provided 20 g; beetles kept at 28°C produced more larval mass than at 32°C. Field experiments were conducted to examine A. tumida reproductive success in full strength bee colonies. Beetles were introduced into hives as egg-infested frames and as adults, and some bee colonies were artificially weakened through removal of sealed brood. Efforts were unsuccessful; no larvae were observed exiting from, or during the inspection of, any hives. Possible reasons for these results are discussed. The variability observed in A. tumida reproduction even in controlled laboratory conditions and the difficulty in causing beetle infestations in field experiments involving full colonies suggest that accurately forecasting the A. tumida severity in such colonies will be difficult. PMID:26470208

  12. Comparative resistance of Russian and Italian honey bees (Hymenoptera: Apidae) to small hive beetles (Coleoptera: Nitidulidae).

    Science.gov (United States)

    Frake, Amanda M; De Guzman, Lilia I; Rinderer, Thomas E

    2009-02-01

    To compare resistance to small hive beetles (Coleoptera: Nitidulidae) between Russian and commercial Italian honey bees (Hymenoptera: Apidae), the numbers of invading beetles, their population levels through time and small hive beetle reproduction inside the colonies were monitored. We found that the genotype of queens introduced into nucleus colonies had no immediate effect on small hive beetle invasion. However, the influence of honey bee stock on small hive beetle invasion was pronounced once test bees populated the hives. In colonies deliberately freed from small hive beetle during each observation period, the average number of invading beetles was higher in the Italian colonies (29 +/- 5 beetles) than in the Russian honey bee colonies (16 +/- 3 beetles). A similar trend was observed in colonies that were allowed to be freely colonized by beetles throughout the experimental period (Italian, 11.46 +/- 1.35; Russian, 5.21 +/- 0.66 beetles). A linear regression analysis showed no relationships between the number of beetles in the colonies and adult bee population (r2 = 0.1034, P = 0.297), brood produced (r2 = 0.1488, P = 0.132), or amount of pollen (P = 0.1036, P = 0.295). There were more Italian colonies that supported small hive beetle reproduction than Russian colonies. Regardless of stock, the use of entrance reducers had a significant effect on the average number of small hive beetle (with reducer, 16 +/- 3; without reducer, 27 +/- 5 beetles). However, there was no effect on bee population (with reducer, 13.20 +/- 0.71; without reducer, 14.60 +/- 0.70 frames) or brood production (with reducer, 6.12 +/- 0.30; without reducer, 6.44 +/- 0.34 frames). Overall, Russian honey bees were more resistant to small hive beetle than Italian honey bees as indicated by fewer invading beetles, lower small hive beetle population through time, and lesser reproduction. PMID:19253612

  13. Endophyte isolate and host grass effects on Chaetocnema pulicaria (Coleoptera: Chrysomelidae) feeding.

    Science.gov (United States)

    Ball, Olivier J P; Gwinn, Kimberly D; Pless, Charles D; Popay, Alison J

    2011-04-01

    Endophytic fungi belonging to the genus Neotyphodium, confer resistance to infected host grasses against insect pests. The effect of host species, and endophtye species and strain, on feeding and survival of the corn flea beetle, Chaetocnema pulicaria Melsheimer (Coleoptera: Chrysomelidae) was investigated. The grass-endophyte associations included natural and artificially derived associations producing varying arrays of common endophyte-related alkaloids or alkaloid groups, peramine, lolitrem B, ergovaline, and the lolines. Preference and nonpreference tests showed that C. pulicaria feeding and survival were reduced by infection of tall fescue with the wild-type strain of N. coenophialum, the likely mechanism being antixenosis rather than antibiosis. In the preference tests, endophyte and host species effects were observed. Of the 10 different Neotyphodium strains tested in artificially derived tall fescue associations, eight strongly deterred feeding by C. pulicaria, whereas the remaining two strains had little or no effect on feeding. Infection of tall fescue with another fungal symbiont, p-endophyte, had no effect. Perennial ryegrass, Lolium perenne L., infected with six strains of endophyte, was moderately resistant to C. pulicaria compared with endophyte-free grass, but four additional strains were relatively inactive. Six Neotyphodium-meadow fescue, Festuca pratensis Huds., associations, including the wild-type N. uncinatum-meadow fescue combination, were resistant, whereas three associations were not effective. Loline alkaloids seemed to play a role in antixenosis to C. pulicaria. Effects not attributable to the lolines or any other of the alkaloids examined also were observed. This phenomenon also has been reported in tests with other insects, and indicates the presence of additional insect-active factors. PMID:21510220

  14. Efficacy of natural products against Callosobruchus chinensis (Coleoptera: Bruchidae) in Nepal.

    Science.gov (United States)

    Regmi, Homan; Kafle, Lekhnath; Gc, Yubak Dhoj; Shih, Cheng Jen

    2012-06-01

    This study was conducted to determine the efficacy of local natural products against the beetle, Callosobruchus chinensis L. (Coleoptera: Bruchidae), in stored chickpea Cicer arietinum L. (Fabaceae) in Chitwan, Nepal. Five natural products and one synthetic pesticide (Malathion) and two storage regimes (aluminum sheet bin vs. jute bags with plastic lining) were tested for their effect on stored pulse with respect to damage by C. chinensis. The five natural products included Xanthoylum armatum DC (Rutaceae) fruit powder, Acorus calamus L. (Araceae) rhizome powder, Cinnamom camphora L. (Lauraceae) balls, oil of Sesamum indicum L. (Pedaliaceae), and leaf powder of Azadirachta indica A. Juss. (Meliaceae). Treatments of stored pulses with natural products or with Malathion all caused significantly higher mortality of C. chinensis at 15 d after treatment (DAT) than stored pulses receiving no treatments. The balls of C. camphora, rhizome powder of A. calamus and sesame oil outperformed all other treatments, including the Malathion at 45 and 75 DAT and resulted in significantly reduced damage or deterioration of stored pulses compared with other treatments. Storage regimes performed similarly, although the jute bags did protect seed integrity for some treatments. Our results indicate that incorporating these natural products into stored pulses can protect the seeds from C. chinensis for up to two generations, something that Malathion cannot do. These products are readily available to most farmers in the region and their use will lead to 1) reduction of losses to significant stored product pests, and 2) a reduction of contamination of foodstuffs and the environment by synthetic pesticides like Malathion. PMID:22812152

  15. Mortality of a wireworm, Agriotes obscurus (Coleoptera: Elateridae), after topical application of various insecticides.

    Science.gov (United States)

    Van Herk, W G; Vernon, R S; Tolman, J H; Saavedra, H Ortiz

    2008-04-01

    Ten insecticides representing seven chemical groups were applied at various concentrations topically by using a Potter Spray Tower to evaluate their relative toxicities on the European wireworm Agriotes obscurus L. (Coleoptera: Elateridae). Wireworms were stored at 15 degrees C after exposure to organophosphate (OP) (chlorpyrifos, diazinon), pyrethroid (tefluthrin), thianicotinoid (thiamethoxam, clothianidin), chloronicotinoid (imidacloprid, acetamiprid), phenyl pyrazole (fipronil), organochlorine (lindane), and spinosyn (spinosad) insecticides, and their postapplication health was evaluated weekly for up to 301 d. LC50, LC90, LT50, and LT90 values were calculated for each chemical except acetamiprid, and compared with those of lindane, clothianidin, and chlorpyrifos. Wireworms exposed to OPs died or recovered more quickly (LT50 < 20 d, LT90 < 50 d), than those exposed to all other insecticides tested except tefluthrin (LT50 = 25.5 d, LT90 = 66.5 d). Wireworms exposed to sublethal concentrations of all neonicotinoids quickly became moribund after application but made a full recovery. Wireworms exposed to fipronil at concentrations near the LC90 value showed no intoxication symptoms for up to 35 d, and they did not recover after symptoms developed. For each chemical, increasing the concentration increased the time required for wireworms to recover but decreased the time required to kill wireworms. Fipronil was highly toxic to wireworms (LC50 = 0.0001%), but acetamiprid (LC50 = 1.82%), imidacloprid (LC50 = 0.83%), tefluthrin (LC50 = 0.23%), diazinon (LC50 = 0.54%), and spinosad (LC50 = 0.51%) were not. The toxicity of both clothianidin (LC50 = 0.07%) and thiamethoxam (LC50 = 0.17%) were similar to those oflindane (LC50 = 0.06%) and chlorpyrifos (LC50 = 0.10%). PMID:18459401

  16. Nutritional Physiology of the Khapra Beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae) Fed on Various Barley Cultivars.

    Science.gov (United States)

    Seifi, S; Naseri, B; Razmjou, J

    2016-02-01

    The Khapra beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae), is known as one of the mostserious pests of grains in many parts of the world. In this study, the effect of nine barley cultivars (‘Bahman’,‘CB-84-10’, ‘Fajr 30’, ‘Makuyi’, ‘Nosrat’, ‘Yousof’, ‘13A1’, ‘18A1’, and ‘19 A1’) and a wheat cultivar (‘MV17’, as a control) was determined on the nutritional indices and digestive enzymatic activity of T. granarium at 33 6 1C,relative humidity of 6565%, and a photoperiod of 14:10 (L:D) h. The highest and lowest values of larval weight gain of sixth instar were detected on wheat (0.757±0.068 mg) and cultivar Bahman (0.342±0.071 mg). Also, T. granarium larvae fed on cultivar Bahman had the lowest value of efficiency of conversion of ingested food(10.90±2.09%) as compared with wheat and other barley cultivars. Also, the highest midgut amylolytic and proteolytic activities of sixth instar were on cultivar Bahman (0.364±0.024 mU/mg and 80.54±1.73 U/mg, respectively)and the lowest activities were on cultivar Nosrat (0.043±0.004 mU/mg and 7.15±0.01 U/mg, respectively).It is concluded that barley cultivar Bahman was the most unsuitable host for feeding of T. granarium. PMID:26612893

  17. 从野生与突变株云芝IBL-04提纯锰过氧化物酶及其表征%Purification and characterization of manganese peroxidases from native and mutant Trametes versicolor IBL-04

    Institute of Scientific and Technical Information of China (English)

    Muhammad Asgher; Muhammad Ramzan; Muhammad Bilal

    2016-01-01

    Extracellular manganese peroxidases (MnPs) produced by native and mutant strains of Trametes versicolor IBL‐04 (EB‐60, EMS‐90) were purified by ammonium sulphate precipitation and dialysis, followed by ion‐exchange and gel‐permeation chromatography. The purified enzymes elucidated a single band in the 43‐kDa region on sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. The optimum pH and temperature of the purified enzymes were found to be 5.0 and 40 °C, respec‐tively. Mutant strain MnPs exhibited a broader active pH range and higher thermal stability than native MnP. Purified MnPs from selected mutants showed almost identical properties to native MnP in electrophoresis, steady‐state kinetics, and metal ion and endocrine‐disrupting compound (EDC) degradation efficiency. Although the fastest reaction rates occurred with Mn2+, MnPs displayed the highest affinity for ABTS, methoxyhydroquinone, 4‐aminophenol and reactive dyes. MnP activity was significantly enhanced by Mn2+and Cu2+, and inhibited in the presence of Zn2+, Fe2+, ethylene‐diaminetetraacetic acid and cysteine to various extents, with Hg2+ as the most potent inhibitory agent. MnPs from all sources efficiently catalyzed the degradation of the EDCs, nonylphenol and triclosan, removing over 80%after 3 h of treatment, which was further increased up to 90%in the presence of MnP‐mediator system. The properties of T. versicolor MnPs, such as high pH and ther‐mal stability, as well as unique Michaelis‐Menten kinetic parameters and high EDC elimination effi‐ciency, render them promising candidates for industrial exploitation.%由野生及突变株云芝IBL-04制得细胞外锰过氧化物酶(MnPs),并经过硫酸铵沉淀、透析、离子交换和凝胶渗透层析法等步骤提纯.纯化的酶在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)上于43 kDa区域呈现单一谱带,它适宜的pH值和温度分别为5.0和40°C.突变株MnPs表现出比野生

  18. Distribución y patrones de diversidad de los Afódidos en la Comunidad de Madrid (Coleoptera, Scarabaeoidea, Aphodiidae, Aphodiinae y Psammodiinae

    Directory of Open Access Journals (Sweden)

    Hortal, J.

    2006-05-01

    Full Text Available In this work we present an updated checklist of the Aphodiidae (Coleoptera, Scarabaeoidea from Comunidad de Madrid (Spain. In addition, the observed and potential distributions of the 70 species found in Madrid are mapped. The potential spatial distributions of species richness, rarity and endemism in this region are also mapped. Finally, we discuss briefly the origin of the observed patterns.

    En este trabajo se presenta un inventario actualizado de los Aphodiidae (Coleoptera, Scarabaeoidea de la Comunidad de Madrid. Además, se presentan mapas de la distribución observada y potencial de las 70 especies encontradas en Madrid, así como de la distribución espacial de la riqueza, rareza, y endemicidad potencial en la región. Finalmente, se discute brevemente el origen de los patrones observados.

  19. Mass Trapping and Classical Biological Control of Rhynchophorus palmarum L. 1794 (Coleoptera: Curculionidae. A hypothesis based in morphological evidences.

    Directory of Open Access Journals (Sweden)

    Flávio Miguens

    2011-07-01

    Resumo. Coleópteros têm sido descritos como pragas e vetores de nematódeos causadores de Anel Vermelho em diversas palmeiras da família Arecaceae. Rhynchophorus palmarum L (Coleoptera: Curculionidae é uma praga que afeta a cocoicultura e outras palmeiras; e, vetor de Bursaphelencus cocophilus (Cobb Baujard (Nematoda, agente etiológico de Anel Vermelho e de outros nematódeos. Atualmente, recomenda-se o emprego de inimigos naturais e parasitas no manejo integrado de pragas; dentre elas, Rhynchophorinae. Armadilhas de coleta massal são recomendadas no manejo integrado de pragas. Nosso estudo relata, na cocoicultura, a eficiência de armadilhas artesanais de baixo custo e a utilização cariomônios (toletes de cana-de-açúcar e cariômonios mais feromônios (toletes de cana-de-açúcar e machos adultos de R. palmarum como atrativos nas armadilhas. Ácaros ectoparasitas foram identificados nestes coleópteros, por microscopia, que podem ser propostos como parte do manejo integrado desta praga. Armadilhas de coleta massal com cariômonios foram eficientes na captura de R. palmarum e outros Curculionidae. No entanto, armadilhas de coleta massal com cariômonios e feromônios aumentaram a atratividade, em relação às primeiras, para este Coleoptera e Cosmopolites sordidus (Germar (Coleoptera: Curculionidae. Eventualmente, exemplares de R. palmarum apresentavam infecção fúngica. Ácaros ectoparasitas infestavam mais de 50% dos exemplares de R. palmarum. A microanatomia destes ácaros e sua interação com R. palmarum foi preliminarmente descrita. Todos os estágios do ciclo de vida destes ácaros foram identificados no compartimento dos élitros. As evidências morfológicas suportam a hipótese de que estes ácaros podem ser empregados no controle biológico de R. palmarum em um programa de manejo integrado.

  20. Notes on Diochus Erichson, Lissohypnus Casey, and Oxybleptes Smetana (Coleoptera: Staphylinidae) in Florida, including a description of a new species of Lissohypnus

    OpenAIRE

    Frank, J. Howard; Kelly, Sandor L.; Almquist, David T.

    2014-01-01

    The known range of Oxybleptes meridionalis Smetana (Coleoptera: Staphylinidae) is expanded in Florida, USA, from Indian River and Manatee counties to now include Brevard, Highlands, Orange, Seminole and Volusia. Oxybleptes davisi (Notman) is confi rmed to exist in Florida, with records from Leon, Liberty and Wakulla counties in the Panhandle, and Orange County in central Florida. Lissohypnus texanus Casey is newly reported from Florida. A new species, Lissohypnus fullertoni, is described from...

  1. Description of a new species of Plesioclytus Giesbert (Coleoptera: Cerambycidae: Cerambycinae) from Georgia, and transfer of the genus to Plesioclytini Wappes and Skelley, new tribe

    OpenAIRE

    Wappes, James E.; Skelley, Paul E.

    2016-01-01

    Plesioclytus morrisi Wappes and Skelley new species (Coleoptera: Cerambycidae) from the Ohoopee dune system in central Georgia is described with comments on the biology of the new species. The taxonomic placement of Plesioclytus Giesbert in the tribe Clytini is questioned as key characters are found to differ from the current characters used to define the tribe in the New World, resulting in its transfer to the newly erected Plesioclytini Wappes and Skelley new tribe, defined herein. Habitat ...

  2. A Temperature-Dependent Development Model for Willow Beetle Species (Coleoptera: Chrysomelidae) in Ireland: Simulation of Phenology/Voltinism in Response to Climate Change

    OpenAIRE

    Pollard, Ciaran P.

    2015-01-01

    Rising fossil fuel prices, energy security and adherence to existing European Union (EU) climate/energy policies means that Ireland must look towards alternative energy sources to meet future demand. Woody biomass in the form of short rotation coppice willow (SRCW) is considered a viable option. SRCW is vulnerable to damage by a range of diseases and pests however. The blue (Phratora vulgatissima) and brown (Galerucella lineola) willow beetles (Coleoptera: Chrysomelidae) are identified econom...

  3. Mortality and impaired development of Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae) induced by extract of sangra d’água Croton urucurana Baill (Euphorbiaceae).

    OpenAIRE

    Gabriel dos Santos Carvalho; Leonardo Santana da Silva; Luciana Barboza Silva; Mayra Layra dos Santos Almeida; Bruno Ettore Pavam; Marize Terezinha Lopes Pereira Peres

    2014-01-01

    Phaseolus vulgaris L., the green bean, is an important source of protein and minerals in the diet of the population. But its productivity is affected by the attack of numerous pests, from emergence to storage, Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae) being the major pest of stored beans. In the search for alternatives to chemical control of this insect and considering the potential biological activity of plant secondary metabolites, this study was conducted in order to evaluate...

  4. A new species of Drapetes Megerle (Coleoptera: Elateridae), with taxonomic summaries and a key to the species of northern North America

    OpenAIRE

    Johnson, Paul J.

    2016-01-01

    Seven species of Drapetes Megerle (Coleoptera: Elateridae) are recognized from northern North America. Drapetes chiricahua new species is described from southeastern Arizona and north-central New Mexico. Drapetes parallelus Van Dyke is a senior homonym of Drapetes paralellus Cobos by variant spelling, and a new synonym of Drapetes cylindricus Fall. Drapetes paralellus Cobos is replaced with Drapetes cobosi new name. Drapetes clarki Bonvouloir is not a North American species and is given a red...

  5. A simultaneous journal / wiki publication and dissemination of a new species description: Neobidessodes darwiniensis sp. n. from northern Australia (Coleoptera, Dytiscidae, Bidessini)

    OpenAIRE

    Lars Hendrich; Michael Balke

    2011-01-01

    Abstract Here, we describe a new Australian species in journal format and simultaneously open the description in a wiki format on the www.species-id.net. The wiki format will always link to the fixed original journal description of the taxon, however it permits future edits and additions to species' taxonomy and biology. The diving beetle Neobidessodes darwiniensis sp. n. (Coleoptera: Dytiscidae, Bidessini) is described based on a single female, collected in a rest pool of the Harriet Creek i...

  6. A scientific note on a new pest for European honeybees: first report of small hive beetle Aethina tumida, (Coleoptera: Nitidulidae) in Italy

    OpenAIRE

    Palmeri, Vincenzo; Scirtò, Giuseppe; Malacrinò, Antonino; Laudani, Francesca; Campolo, Orlando

    2015-01-01

    International audience The small hive beetle (SHB), Aethina tumida Murray(Coleoptera Nitidulidae), is an important pest of honeybeesin many parts of the world. The beetle is native tosub-Saharan Africa and was unintentionally introducedinto USA (1996), Australia (2001) and in Canada(2002) (Ellis and Munn, 2005; Neumann and Ellis2008). In Europe the SHB was discovered in 2005 ina consignment of queens imported from Texas (USA)into Portugal and destroyed by the Portuguese NationalVeterinaria...

  7. Plant compounds insecticide activity against Coleoptera pests of stored products Compostos de plantas com atividade inseticida a coleópteros-praga de produtos armazenados

    OpenAIRE

    Márcio Dionizio Moreira; Marcelo Coutinho Picanço; Luiz Cláudio de Almeida Barbosa; Raul Narciso Carvalho Guedes; Mateus Ribeiro de Campos; Gerson Adriano Silva; Júlio Cláudio Martins

    2007-01-01

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed...

  8. Biological and Morphological Aspects of Anoxia (Mesanoxia) matutinalis ssp. matutinalis Castelnau, 1832 (Coleoptera, Melolonthidae), a Pest of Apulian Pinewoods in Southern Italy

    OpenAIRE

    Eustachio Tarasco; Oreste Triggiani

    2011-01-01

    Huge defoliations by Anoxia (Mesanoxia) matutinalis spp. matutinalis Castelnau, 1832 (Coleoptera, Scarabeidae) have been observed in recent years in the coastal pinewoods (Pinus halepensis, P. pinea) of the southern Apulia Region of Italy. Starting from adults collected in the field, insect breeding was conducted in the laboratory, following the life-cycle from ovideposition to larval development and emergence. Observations were performed in the laboratory and the field to study the different...

  9. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae. A atividade inseticida de Toona sureni (Blume Merr. foi avaliada considerando repelência, mortalidade e a produção de progênie de adultos F1 de Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Extrato seco de sementes

  10. A diversidade inventarial de Coleoptera (Insecta em uma paisagem antropizada do Bioma Araucária The inventory diversity of Coleoptera (Insecta of an anthropized landscape in the Biome Araucaria

    Directory of Open Access Journals (Sweden)

    Norma G. Ganho

    2005-12-01

    Full Text Available Como parte do Projeto PROVIVE, foram analisadas a riqueza de espécies, a composição taxonômica, a proporção de espécies raras e a constância taxonômica ao nível de família relacionada à riqueza de espécies, em comunidades de Coleoptera, em Vila Velha, Ponta Grossa, Paraná. Os dados foram obtidos a partir de coletas através de armadilha malaise no estrato do sub-bosque de cinco áreas com diferentes graus de intervenção antrópica, de setembro de 1999 a agosto de 2000. As 52 semanas de amostragem nas cinco áreas resultaram na coleta de 10.822 indivíduos de 1659 espécies. Todas as áreas apresentaram alta riqueza de espécies e diversidade, como indicado por vários índices. A área em estágio mais avançado de sucessão vegetal foi menos rica do que aquelas em estágio inicial/intermediária. De acordo com diferentes estimadores de riqueza de espécies, o número de espécies coletadas poderia aumentar de 22-123% com o aumento do esforço de coleta. As áreas menos conservadas foram mais ricas em espécies raras ("singletons", "doubletons" e únicas do que as mais conservadas. Nas cinco áreas houve uma constância taxonômica entre as famílias mais ricas (Curculionidae, Chrysomelidae, Cerambycidae, Staphylinidae, Mordelidae, Elateridae, Scarabaeidae, Coccinellidae e Tenebrionidae envolvendo 60% do total de espécies, como observado para a abundância de indivíduos. A existência de um padrão de constância taxonômica de famílias, quando considerados 60% da riqueza de espécies e/ou de abundância de indivíduos por local, poderá tornar mais fácil e rápido o estudo de comunidades de Coleoptera, habilitando a ordem a ser um táxon indicador de condições ambientais de áreas florestadas.The species richness, taxonomic composition, rare species, and taxonomic constancy at family level were studied in communities of Coleoptera in Vila Velha, Ponta Grossa, Paraná, as part of PROVIVE project. The data were gathered

  11. Attraction of Astylus variegatus (Germ. (Coleoptera: Melyridae by volatile floral attractants Atração de Astylus variegatus (Germ. (Coleoptera: Melyridae por atraentes florais voláteis

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    Maurício Ursi Ventura

    2007-06-01

    Full Text Available The beetle Astylus variegatus (Germ. (Coleoptera: Melyridae is frequently found in flowers feeding on pollen. Responses of A. variegatus to volatile floral attractants were studied in common beans (Phaseolus vulgaris L. fields. Traps originally designed to capture Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, consisted of plastic bottles (2 L with 150 holes (5-mm diameter yellow gold painted and containing inside a plastic strip (3.5 ´ 25 cm with Lagenaria vulgaris (L. powder (0.28% B cucurbitacin - feeding stimulant and arrestant for diabroticites sprayed with carbaril insecticide. Treatments consisted of 1,4-dimethoxybenzene (one or two dispensers per trap, 1,4-dimethoxybenze + indole, 1,4-dimethoxybenzene + cinnameldehyde and control. Volatile average release rates (over ten days was approximately 32 mg day-1 per dispenser under laboratory conditions. 1,4-dimethoxybenzene-lured traps caught significantly more beetles than the control, three and seven days after trap setting. Ten days after the onset of the experiment, there were no differences in number of beetles caught by treatments. Captures were higher in the 1,4-dimethoxybenzene + cinnamaldehyde treatment than in 1,4-dimethoxybenzene only in the first assessment. Adding indole to 1,4-dimethoxybenzene did not improve beetle captures.O besouro Astylus variegatus (Germ. (Coleoptera: Melyridae é freqüentemente encontrado em flores onde se alimenta de pólen. Respostas de A. variegatus a atraentes voláteis florais foram estudadas em campos de feijão, Phaseolus vulgaris L. Armadilhas, originalmente desenvolvidas para capturar Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, consistiram de garrafas plástica (2 L com 150 perfurações (5 mm de diâmetro pintadas com tinta amarelo ouro contendo no seu interior uma fita plástica (3,5 ´ 25,0 cm com pó seco de frutos de Lagenaria vulgaris (L. (0,28% de cucurbitacina B estimulante alimentar e arrestante para diabrotic

  12. Eficiência do inseticida clorpirifós no controle de larvas de Diabrotica speciosa (Germ. (Coleoptera:Chrysomelidae na cultura da batata Performance of the insectide chlorpyrifos to control Diabrotica speciosa (Germ. (Coleoptera:Chrysomelidae in potato crop

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    Luiz Antonio Salles

    1999-06-01

    Full Text Available Foi avaliado o desempenho do inseticida clorpirifós no controle de larvas de Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, na cultura da batata. O experimento foi desenvolvido na safra de primavera, quando ocorre maior incidência de insetos pragas e, conseqüentemente, danos nos tubérculos de batata no Rio Grande do Sul. As diferentes dosagens e formulações dos inseticidas foram aplicadas no sulco de plantio, utilizando-se clorpirifós (2, 3 e 4 l i.a./ha de Lorsban 480 CE e 2 e 3kg i.a./ha de Lorsban 10 G e como padrão, forate (2kg i.a./ha de Granutox 50G. Os melhores controles ocorreram nos tratamentos com Granutox 50G, na dosagem de 2kg i.a./ha; seguido por Lorsban 10 G, 3 e 2kg i.a./ha e Lorsban 480 CE, 4 e 3l i.a./ha.A field trial was conducted to evaluate the performance of the insecticide chlorpyrifos in two formulations, emulsifiable and granular, for the control of Diabrotica speciosa (Germ. (Coleoptera:Chrysomelidae in potato. The field trial was conducted during the spring season when incidence of insect pests and, consequently, damage are high in Rio Grande do Sul state, Brazil. The different dosages and formulations of insecticides were applied in the planting furrow utilizing chlorpyrifos (2, 3 and 4l i.a./ha of Lorsban 480 CE and 2 and 3kg i.a./ha de Lorsban 10G and the standard phorate (2kg i.a./ha of Granutox 50G. Best field control performances were obtained with Granutox 50G, in the dosage of 2kg i.a./ha; Lorsban 10G, 3 and 2kg i.a./ha, followed by Lorsban 480 CE, 4 and 3l i.a./ha.

  13. Hexamermis paranaense new species (Nematoda, Mermithidae: a parasite of Diloboderus abderus (Coleoptera, Scarabaeidae in Argentina Hexamermis paranaense sp. nov. (Nematoda, Mermithidae: parasito de Diloboderus abderus (Coleoptera, Scarabaeidae en Argentina

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    María Fernanda Achinelly

    2008-12-01

    Full Text Available A new species, Hexamermis paranaense n. sp. (Nematoda, Mermithidae, a parasite of larvae of Diloboderus abderus Sturm, 1826 (Coleoptera, Scarabaeidae is described and illustrated. This new species is characterized by amphids small, amphidial opening pocket-shaped, the anterior portion of the vagina muscularized and slightly protruding with a descending branch forming a loop before joining the uterus, and three rows of genital papillae: the ventrolateral divided in two rows with eight papillae in the outer row and with six papillae in the inner one; the ventral row with four pairs and one single preanal papillae, and with two pairs, a triplete, one pair, a single and one pair postanal papillae.Una nueva especie, Hexamermis paranaense sp. nov. (Nematoda, Mermithidae, parásito de larvas del gusano blanco, Diloboderus abderus Sturm, 1826 (Coleoptera, Scarabaeidae, se describe e ilustra. Esta nueva especie se caracteriza por poseer anfidos pequeños, con la apertura en forma de bolsillo, parte anterior de la vagina muscular, con una rama que desciende y forma un lazo antes de la conexión al útero, tres hileras de papilas genitales: la hilera de posición ventro-lateral dividida en dos hileras con ocho papilas en la hilera externa y con seis papilas en la interna; la hilera ventral con una papila, seguida de cuatro pares, todas en posición preanal y dos pares, interrumpidos por tres papilas, seguida de un par, una simple, y un par, todas de posición postanal.

  14. Morphological and isozymic banding pattern study of white grubs (Coleoptera: Melolonthidae as pest of bark crop in mounth Merapi’s slope.

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    SUGIYARTO

    2008-07-01

    Full Text Available White grub (Coleoptera: Melolonthidae is a group of soil pest at any agrosystem., especially at Salak pondoh (Salacca zalacca (Gaert. Voss. crop. The characteristics of this specimen were very crucial to be studied in order to find the exact biocontrol. The aim of this research was to know the characteristics of white grubs (Melolonthidae: Coleoptera based on morphological and isozyme banding patterns. This research was conducted on August - November 2007 at Sleman and Magelang districts for the morphological purposes, while for the isozyme data were conducted at Sub Laboratory Biology, Central Laboratory of Sebelas Maret University Surakarta. Sample was taken by using stratified random sampling method, on five stations. Polyacrylamide gel electrophoresis (PAGE using the vertical type was taken to isozyme analysis. The enzyme used in this research were peroxidase and esterase to detect the isozyme banding patterns. The results showed that there was no morphological variation of white grubs (Melolonthidae: Coleoptera at salak pondoh agroecosystem in Mounth Merapi’s slope. Based on this character, there was one species of white grub found, i.e. Holotrichia javana. There was a genetic variation based on the variation of isozyme banding patterns.

  15. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    Science.gov (United States)

    Hönnicke, M. G.; Cusatis, C.; Rigon, L.; Menk, R.-H.; Arfelli, F.; Foerster, L. A.; Rosado-Neto, G. H.

    2010-08-01

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures ( Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  16. Predation potential of Chilocorus cacti (Coleoptera: Coccinellidae) to the prickly pear cacti pest Dactylopius opuntiae (Hemiptera: Dactylopiidae).

    Science.gov (United States)

    Flores, A; Olvera, H; Rodríguez, S; Barranco, J

    2013-08-01

    Functional response of the predator Chilocorus cacti (Linnaeus) (Coleoptera: Coccinellidae) on five densities of Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae) female adults was assessed under laboratory conditions. The searching efficiency of C. cacti significantly decreased as prey density increased. The logistic regression for the predator had a negative and significant linear parameter indicating a type II functional response. Non-linear regression for Holling predator equation estimated a handling time of 1.79 ± 0.129 h and attack rate coefficient of 0.1003 ± 0.030. Most of this handling time was because the predator spent a lot of time removing the waxy coating that protects adult females of D. opuntiae. Chilocorus cacti consumes females of D. opuntiae in their reproductive stage; therefore, it could be an effective natural enemy to suppress or regulate low density populations of D. opuntiae, preventing them to reach high densities. PMID:23949861

  17. Characterization of the proteases in the midgut of the xylophagous larvae of Oemona hirta (Coleoptera:Cerambycidae)

    Institute of Scientific and Technical Information of China (English)

    Brian David Shaw; John Tane Christeller

    2009-01-01

    The protein digestive capability oftbe larvae of the longhorn beetle (Oemona hirta,Coleoptera:Cerambycidae,Fabricius,1775) was investigated.This species feeds only on wood where there is a high proportion of vascular tissue.The pH of the midgut,the major digestive organ,was alkaline and protein hydrolysis was maximal at alkaline pH.Use of specific synthetic peptide substrates showed that the major protease activities were the endopeptidases,trypsin and chymotrypsin-like activity,and the exopeptidase,leucine aminopeptidase and the pH curves corresponded to that with protein substrate.Studies using a range ofsefine protease inhibitors as well as specific inhibitors ofmetalloproteases,cysteine proteases and aspartate proteases confirmed a serine protease-based digestive system similar to earlier reports of sapwood-feeding Cerambycids.Control of these insect pests using protease inhibitors is discussed.

  18. Toxicities of azadirachtin and polychlorinated petroleum Hydrocarbon against resist and susceptible strains of tribolium castaneum (coleoptera: tenebrionidae) adults

    International Nuclear Information System (INIS)

    The LC/sub 50/ values for malathion-resistant (PAK) and organo-50 phosphate-susceptible (FSS-II) strains of red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) adult beetles were determined through filter paper impregnated method against azadirachtin (Nimbokil 60 EC) and polychlorinated petroleum hydrocarbon (Tenekil 100 EC). The LC values of these insecticides were worked out as 12830 and 50 9331 ppm for azadirachtin and 5148 and 4047 ppm for Tenekil 100 EC against PAK and FSS-II strains, respectively. The results revealed that polychlorinated petroleum hydrocarbon was more toxic than the azadirachtin. Furthermore, both the insecticides were equally toxic to the adult beetles of T. castaneum as the difference was non-significant because of overlapping 95% FLs to LC./sub 50/. (author)

  19. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    International Nuclear Information System (INIS)

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures (Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  20. The effects of temperature, diet, and other factors on development, survivorship, and oviposition of Aethina tumida (Coleoptera: Nitidulidae).

    Science.gov (United States)

    Meikle, William G; Patt, Joseph M

    2011-06-01

    Developmental rate and survivorship of small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), life stages were measured across different temperatures (21, 25, 28, 32 and 35 degrees C) and diets, which included natural and artificial pollen, honey, and bee pupae. Temperature affected hatch success, time to hatching, and larval growth. Eggs hatched in 61 h at 21 degrees C but in Beetles lived longer at 28 degrees C or lower but produced the most eggs per female, regardless of diet, at 32 degrees C. Beetle density influenced fecundity: beetles kept at three pairs per vial laid 6.7 times more eggs per female than those kept as single pairs. Overall, beetles fared best at 28-32 degrees C with mortality of all stages highest at 35 degrees C. PMID:21735891

  1. Genetic differentiation among populations of the beetle Bolitophagus reticulatus (Coleoptera: tenebrionidae) in a fragmented and a continuous landscape.

    Science.gov (United States)

    Knutsen, H; Rukke, B A; Jorde, P E; Ims, R A

    2000-06-01

    The effect of habitat fragmentation on genetic differentiation among local populations of the fungivorous beetle Bolitophagus reticulatus (Coleoptera: Tenebrionidae) was studied in two contrasting landscapes: one heavily fragmented with forest fragments of variable size surrounded by inhabitable agricultural fields, the other an old forest providing a continuous habitat. The genetic structure of the beetle within each of the two contrasting areas was investigated by means of protein electrophoresis, screening four polymorphic loci in 20 populations from each area. In both areas there were significant genetic differences among local populations, but on average differentiation in the fragmented area was three times greater than in the continuous one, strongly indicating a genetic isolation effect of habitat fragmentation. These genetic results are in accordance with previous studies on dispersal in this species. PMID:10886382

  2. Biological control agent of larger black flour beetles (Coleoptera: Tenebrionidae): a nuisance pest developing in cotton gin trash piles.

    Science.gov (United States)

    Nansen, Christian; Stokes, Bryan; James, Jacob; Porter, Patrick; Shields, Eilson J; Wheeler, Terry; Meikle, William G

    2013-04-01

    The larger black flour beetles, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), feeds on saprophytic fungi found in gin trash piles and occasionally becomes a nuisance pest in adjacent homes and businesses. The potential of Steinernema carpocapsae 'NY 001,' as a potential control agent of larger black flour beetle under experimental conditions was examined with particular reference to the importance of soil moisture content. Without prospects of insecticides being labeled for control of larger black flour beetle in gin trash, the data presented here support further research into applications of entomopathogenic nematodes underneath gin trash piles as a way to minimize risk of larger black flour beetle populations causing nuisance to nearby homes and businesses. PMID:23786050

  3. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae

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    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.

  4. Parasitylenchus bifurcatus n. sp. (Tylenchida: Allantonematidae parasitizing Harmonia axyridis (Coleoptera: Coccinellidae

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    Poinar George O

    2012-10-01

    Full Text Available Abstract Background The harlequin ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae is native to central and eastern Asia and was purposely introduced into Europe to control aphids. While it proved to be a good biological control agent, its rapid spread and buildup of large populations made it a nuisance, since it overwinters in homes, emits unpleasant odors, stains fabrics, occasionally bites humans and feeds on apples, pears and grapes. Aside from the above, the ravenous appetite of H. axyridis results in their consumption of harmless native insects, including even other ladybird beetles. A study of the natural enemies of H. axyridis in Denmark revealed the presence of nematodes. The present study describes this nematode parasite and discusses aspects of its development and ecology. Methods Adult harlequin ladybird beetles were collected from March to November from four localities in Copenhagen on different plant species. In addition, groups of last-instar larvae and pupae (n = 50 were examined for the presence of nematodes. Living and recently dead nematodes were removed from adult H. axyridis in 0.5% saline solution, the nematodes were then heat killed (at 75C, fixed in 5% formalin and transferred to glycerin on slides for further examination and measurements. Results A new species of Allantonematidae (Tylenchida, Parasitylenchus bifurcatus n. sp., is described from adults of the harlequin ladybird, Harmonia axyridis in Denmark. The new species is characterized by a straight stylet lacking basal thickenings, a bursa and a forked tail tip in the vermiform (infective females and juvenile males. The new species is compared with P. coccinellinae previously described from ladybird beetles in France. Parasitism resulted in depletion of the fat body and partial or complete atrophy of the reproductive organs of the beetles. Infections occurred throughout the year with rates of parasitism reaching up to 35%. The rate increased to 60

  5. Entomofauna Associada a Galhos de Acacia mangium Willd. Roletados por Oncideres saga (Dalman (Coleoptera: Cerambycidae

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    Gláucia Cordeiro

    2010-04-01

    Full Text Available O estudo da entomofauna associada aos galhos e fustes roletados por Oncideres saga (Dalman é importante para conhecermos seus possíveis inimigos naturais. Portanto, este trabalho teve como objetivo registrar os insetos associados aos galhos e fustes de Acacia mangium Willd. roletados por O. saga, em Coimbra-MG. Galhos e fustes roletados de A. mangium foram coletados de janeiro a abril de 2007. Este material foi vistoriado, armazenados em sacolas, feitas com tela plástica, e mantidos em sala com condições controladas (25,4±0,3°C e 66,7±1,4%. Constatou-se a presença de uma espécie, não determinada, de Scolytidae e a emergência de quatro espécies de Cerambycidae: Engyum quadrinotatum Thomsom; Eburodacrys sexmaculata (Olivier; Achryson surinamum (Linnaeus e Neoclytus pusillus (Laporte & Gory. Isto posto, fica evidenciada a necessidade de estudos com o objetivo de verificar qual o comportamento destes insetos em relação ao serrador O. saga.Insects associated with branches of Acacia mangium Willd. girdled by Oncideres saga (Dalman (Coleoptera: CerambycidaeAbstract. The study of the insects associated with branches and stems girdled by Oncideres saga (Dalman is important to know its possible natural enemies. Therefore, these work had the objective of register the insects associated with branches and stems girdled of Acacia mangium Willd. by this twig girdler beetle, in Coimbra, state of Minas Gerais, Brazil. Stems and branches of A. mangium were collected in January/2007 to April/2007. This material has been inspected, stored in plastic bags, and kept in a room with controlled conditions (25.4 ± 0.3°C and 66.7 ± 1.4%. It was noted the presence of a non-determined species of Scolytidae and the emergence of four species of Cerambycidae: Engyum quadrinotatum Thomsom; Eburodacrys sexmaculata (Olivier; Achryson surinamum (Linnaeus and Neoclytus pusillus (Laporte & Gory. It can be concluded that studies are needed with the objective of

  6. Karyotypes of some medium-sized Dytiscidae (Agabinae and Colymbetinae (Coleoptera

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    Robert Angus

    2013-06-01

    Full Text Available An account is given of the karyotypes of 29 species of medium sized Dytiscidae (Coleoptera. Of the 20 species of Agabus Leach, 1817, 18 have karyotypes comprising 21 pairs of autosomes and sex chromosomes which are either X0(♂ or XX (♀. These species are A. serricornis (Paykull, 1799, A. labiatus (Brahm, 1791, A. congener (Thunberg, 1794, A. lapponicus (Thomson, 1867, A. thomsoni (J. Sahlberg, 1871, A. confinis (Gyllenhal, 1808, A. sturmii (Gyllenhal, 1808, A. bipustulatus (Linnaeus, 1767, A. nevadensis Håkan Lindberg, 1939, A. wollastoni Sharp, 1882, A. melanarius Aubé, 1837, A. biguttatus (Olivier, 1795, A. binotatus Aubé, 1837, A. affinis (Paykull, 1798, A. unguicularis (Thomson, 1867, A. ramblae Millan & Ribera, 2001, A. conspersus (Marsham, 1802 and A. nebulosus (Forster, 1771. However two species, A. infuscatus Aubé, 1838 and A. adpressus Aubé, 1837, have developed a neo-XY system, with karyotypes comprising 21 pairs of autosomes and XY sex chromosomes (♂. No chromosomal differences have been detected between typical A. bipustulatus and A. bipustulatus var. solieri Aubé, 1837, nor have any been found between the three species of the A. bipustulatus complex (A. bipustulatus, A. nevadensis and A. wollastoni. The four species of Colymbetes Clairville, 1806, C. fuscus (Linnaeus, 1758, C. paykulli Erichson, 1837, C. piceus Klug, 1834 and C. striatus (Linnaeus, 1758 have karyotypes comprising 20 pairs of autosomes and sex chromosomes which are X0 (♂, XX (♀. Two of the species of Rhantus Dejean, 1833, R. exsoletus (Forster, 1771 and R. suturellus (Harris, 1828 have karyotypes comprising 20 pairs of autosomes and X0/XX sex chromosomes, but the other three species, R. grapii (Gyllenhal, 1808, R. frontalis (Marsham, 1802 and R. suturalis (Macleay, 1825 have 22 pairs of autosomes and X0/XX sex chromosomes. Agabus congener and Rhantus suturellus may have one B-chromosome. Nine of the species have previously published karyotype data but

  7. Susceptibility of Agrilus planipennis (Coleoptera: Buprestidae) to Beauveria bassiana and Metarhizium anisopliae.

    Science.gov (United States)

    Liu, Houping; Bauer, Leah S

    2006-08-01

    The susceptibility of Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) to selected strains of the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin was evaluated through bioassays with direct immersion or foliar exposure under laboratory conditions. Results showed that A. planipennis adults were susceptible to B. bassiana and M. anisoplae. Significant time-mortality response was found for each isolates. Isolate B. bassiana GHA killed A. planipennis adults at a faster rate compared with other isolates tested, with the lowest average time-to-death values. The LC50 values estimated under direct immersion method ranged from 1.7 x 10(5) to 1.9 x 10(7), 3.5 x 10(4) to 5.3 x 10(5), and 4.1 x 10(3) to 2.9 x 10(5) conidia/ml for B. basissiana and from 3.2 x 10(6) to 1.1 x 10(7), 4.5 x 10(3) to 4.5 x 10(5), and 1.4 x 10(2) to 1.2 x 10(5) conidia/ml for M. anisopliae at 4, 5, and 6 d after treatment, respectively. By days 5 and 6, B. bassiana GHA outperformed all other isolates tested except ARSEF 7234, followed by ARSEF 7152, 6393, and 7180. Significant concentration-mortality response was also observed for two B. bassiana GHA formulations, BotaniGard ES and Mycotrol O, and M. anisopliae F52 when insects were treated through foliar exposure. The LC50 values ranged from 114.5 to 309.6, 18.4 to 797.3, and 345.3 to 362.0 conidia/cm2 for BotaniGard, Mycotrol, and M. anisopliae F52, respectively. Based on the results of these bioassays, the efficacy of both B. bassiana GHA formulations and M. anisopliae F52 were similar against adult A. planipennis. The potential use of entomopathogenic fungi for management of A. planipennis in North America is discussed. PMID:16937660

  8. Diversity and altitudinal distribution of Chrysomelidae (Coleoptera in Peregrina Canyon, Tamaulipas, Mexico

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    Uriel Jeshua Sánchez-Reyes

    2014-06-01

    Full Text Available The Chrysomelidae (Coleoptera is a highly speciose family that has been poorly studied at the regional level in Mexico. In the present study, we estimated species richness and diversity in oak-pine forest, Tamaulipan thorny scrub and in tropical deciduous forests in Peregrina Canyon within the Altas Cumbres Protected Area of the northeastern state of Tamaulipas, Mexico. Sampling of Chrysomelidae consisted of five sweep net samples (200 net sweeps within each of three sites during four sample periods: early dry season, late dry season, early wet season, and late wet season. Species were identified and total numbers per species were recorded for each sample. A total of 2,226 specimens were collected belonging to six subfamilies, 81 genera and 157 species of Chrysomelidae from the study area. Galerucinae was the most abundant subfamily with 1,828 specimens, representing 82.1% of total abundance in the study area. Lower abundance was recorded in Cassidinae (8.5%, Eumolpinae (3.6%, Cryptocephalinae (2.2%, Chrysomelinae (2.2%, and finally Criocerinae (1.3%. The highest species richness was also presented in the subfamily Galerucinae with 49% of the total obtained species followed by Cassidinae (20%, Cryptocephalinae (9.7%, Eumolpinae (9.7%, Chrysomelinae (6.5% and Criocerinae (5.2%. The most common species were Centralaphthona fulvipennis Jacoby (412 individuals, Centralaphthona diversa (Baly (248, Margaridisa sp.1 (219, Acallepitrix sp.1 (134, Longitarsus sp.1 (104, Heterispa vinula (Erichson (91, Epitrix sp.1 (84 and Chaetocnema sp.1 (72. Twenty-two species were doubletons (1.97% of total abundance and 52 were singletons (2.33%. The estimated overall density value obtained was 0.0037 individuals/m2. The greatest abundance and density of individuals were recorded at the lowest elevation site. However, alpha diversity increased with increasing altitude. Similarity values were less than 50% among the three sites indicating that each site had distinct

  9. Oryctini (Coleoptera: Scarabaeidae: Dynastinae da coleção de invertebrados do Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas, Brasil Oryctini (Coleoptera: Scarabaeidae: Dynastinae of the invertebrate collection of the National Institute of Research of Amazonia (INPA, Manaus, Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Hector Jaime Gasca

    2009-09-01

    Full Text Available A Coleção de Invertebrados do Instituto Nacional de Pesquisas da Amazônia (INPA possui 554 espécimes de Oryctini (Coleoptera: Scarabaeidae: Dynastinae, pertencentes a 7 gêneros, 16 espécies e 2 subespécies. As distribuições geográficas das espécies são fornecidas, sendo que 97% do material examinado procedem de coletas feitas em diferentes locais da Amazônia brasileira.The Invertebrate Collection of the Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas, Brazil, has 554 specimens of Oryctini (Coleoptera: Scarabaeidae: Dynastinae, belonging to seven genera, 16 species and two subspecies. Information about geographical distribution are provided, of which 97% of the material examined was collected from several places in the Brazilian Amazon.

  10. Occurrence and biology of Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae) parasitising different species of Coccinellidae (Coleoptera) in Neotropical region Ocorrência e biologia de Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae) parasitando diferentes espécies de Coccinellidae (Coleoptera) na região Neotropical

    OpenAIRE

    RB Silva; I Cruz; MLC Figueiredo; AG Pereira; AM Penteado-Dias

    2012-01-01

    Surveys on Coccinellidae (Coleoptera) in Sete Lagoas city, Minas Gerais state, Brazil, indicated the parasitism of adults of the species Coleomegilla maculata De Geer, 1775, Eriopis connexa (Germar, 1824) and Olla v-nigrum (Mulsant, 1866), by Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae). Since then, the parasitoid have been maintained in its original hosts at the Insect Rearing Laboratory - LACRI of the Brazilian Agricultural Research Institution - Embrapa Milh...

  11. Fauna de Coleoptera no Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brasil: abundância e riqueza das famílias capturadas através de armadilhas de solo Coleoptera fauna in the Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brazil: abundance and family richness captured with pitfall traps

    OpenAIRE

    Renato C. Marinoni; Norma G. Ganho

    2003-01-01

    This paper is a part of the studies on the Coleoptera fauna from Vila Velha, Ponta Grossa, Parana gathered through malaise and pitfall traps in sites with different floristic conditions. The present study deal with the data of pitfall trap captures, installed close to the malaise traps. The data were obtained weekly (52 samples), from September 1999 to August 2000. This survey was carried out on five areas, three of them with different plant succession stages (initial, intermediate, and advan...

  12. Biological and Morphological Aspects of Anoxia (Mesanoxia) matutinalis ssp. matutinalis Castelnau, 1832 (Coleoptera, Melolonthidae), a Pest of Apulian Pinewoods in Southern Italy Aspetos da Biologia e Morfologia da Anoxia (Mesanoxia) matutinalis ssp. matutinalis Castelnau, 1832 (Coleoptera, Melolonthidae), Praga nos Pinhais da Apúlia, Sul de Itália

    OpenAIRE

    Eustachio Tarasco; Oreste Triggiani

    2011-01-01

    Huge defoliations by Anoxia (Mesanoxia) matutinalis spp. matutinalis Castelnau, 1832 (Coleoptera, Scarabeidae) have been observed in recent years in the coastal pinewoods (Pinus halepensis, P. pinea) of the southern Apulia Region of Italy. Starting from adults collected in the field, insect breeding was conducted in the laboratory, following the life-cycle from ovideposition to larval development and emergence. Observations were performed in the laboratory and the field to study the different...

  13. Levantamento da fauna de Coleoptera que habita a carcaça de Sus scrofa L., em Curitiba, Paraná A study of the Coleoptera (Insecta fauna that inhabits Sus scrofa L. carcass in Curitiba, Paraná

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    Kleber Makoto Mise

    2007-09-01

    Full Text Available Este trabalho visou levantar a fauna de Coleoptera associada à carcaça de Sus scrofa L.,1758, espécie utilizada como modelo em Entomologia Forense. O acréscimo ou a substituição seqüencial das espécies de insetos pode ser utilizado para estimar o intervalo post mortem (IPM. O experimento foi realizado no Centro Politécnico (UFPR, de setembro de 2005 a setembro de 2006. A cada estação foi sacrificado um suíno de 15 kg no local, colocado em gaiola. A captura dos insetos foi realizada diariamente em bandeja posicionada abaixo da carcaça e em armadilha tipo Shannon modificada, e a cada 14 dias em cinco armadilhas do tipo pit-fall. Foram coletados 4.360 Coleoptera, pertencentes a 112 espécies de 26 famílias, 12 consideradas de importância forense. A coleta ativa realizada na bandeja foi responsável pela maior captura (2.023 espécimes, seguida pela armadilha Shannon modificada (2.016 espécimes e por último pelas do tipo pit-fall (324 espécimes. Staphylinidae foi mais coletada na bandeja e Shannon modificada, e Silphidae na armadilha pit-fall. Os principais hábitos encontrados foram predador/parasita (55%e onívoro (38,05%, com poucas espécies consideradas necrófagas (1,31%.This paper sought to assess the Coleoptera fauna associated with carcasses of Sus scrofa L., 1758, which is usually used as model in Forensic Entomology. The addition and sequential substitution of insect species could be used to estimate the post mortem interval (PMI. The present study took place in Centro Politécnico (UFPR, between september 2005 to september 2006. A pig weighting 15 kg was sacrificed each season and put inside a cage. Sampling is made daily in a tray placed below the carcass and in a Shannon modified trap, and each 14 days in five pit-fall traps. 4,360 beetles were collected, belonging to 112 species of 26 families, 12 were considered of forensic potential. The active collecting made in the tray was responsible for the largest number of

  14. Os besouros (Insecta: Coleoptera na concepção dos moradores de Pedra Branca, Santa Terezinha, Estado da Bahia = The term “beetle” (Insecta: Coleoptera as perceived and used by dwellers of Pedra Branca, Bahia, Brazil

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    Eraldo Medeiros Costa Neto

    2006-01-01

    Full Text Available Besouros (Insecta: Coleoptera são percebidos, conhecidos e utilizados por moradores do povoado de Pedra Branca, localizado no Estado da Bahia, Brasil. O trabalho de campo foi realizado entre abril e dezembro de 2004. Os dados foram obtidos com 49 indivíduos (20 do sexo masculino e 29 do sexo feminino por meio de entrevistas semiestruturadas e gravadas, seguindo-se uma abordagem emicista. Os resultados mostram que o genérico “besouro” é usado como uma categoria etnotaxonômica que reúne organismos sistematicamente não relacionados, além dos coleópteros da classificação lineana. Foramcitados 43 nomes locais de besouros. Os informantes atribuíram características qualitativas aos “besouros”, muitas das quais são antropomórficas. Algumas etnoespécies de “besouros” causam danos aos moradores de Pedra Branca, uma vez que ameaçam a saúde, os materiais,os cultivos locais e plantas frutíferas. Registrou-se informação sobre a história natural de “besouros” culturalmente importantes. Conclui-se que o conhecimento etnoentomológico dos moradores de Pedra Branca sobre besouros é importante como uma fonte de informação sobre as espécies locais.Fieldwork was carried out from April to December 2004. Data were obtained from 49 individuals (20 men and 29 women by means of open-ended tape-recorded interviews and following an emicist approach. Results show that the generic taxon “beetle” is used as an ethnotaxonomic category that brings together not systematically related organisms, besides the Coleoptera of the Linnaean classification. A total of 43 beetle local names were cited. People have attributed qualitative characteristics to these “beetles”, many of which are anthropomorphic. “Beetles” cause damages to human beings since they harm their health, materials, and local crops and fructiferous plants. Information on the natural history of culturally significant “beetles” was recorded. It is concluded that

  15. Notes on three braconid wasps (Hymenoptera: Braconidae, Doryctinae) parasitizing oak long-horned beetle, Massicus raddei (Coleoptera: Cerambycidae), a severe pest of Quercus spp. in China, together with the description of a new species.

    Science.gov (United States)

    Cao, Liangming; Yang, Zhongqi; Tang, Yanlong; Wang, Xiaoyi

    2015-01-01

    Three species of Doryctinae (Hymenoptera: Braconidae) parasitize larvae of oak longhorn beetle Massicus raddei Blessig (Coleoptera: Cerambycidae), a serious wood borer pest in North China. Rhoptrocentrus quercusi sp. nov., is described as a new species and Doryctes petiolatus Shestakov, as well as Zombrus bicolor (Enderlein). The three species are idiobiont ectoparasitoids, and may have potential for biological control of oak longhorn beetle. PMID:26624143

  16. Rozšíření rýhovců Rhysodes sulcatus (Fabricius, 1787) a Omoglymmius germari (Ganglbauer, 1892) (Coleoptera: Rhysodidae) v České republice

    Czech Academy of Sciences Publication Activity Database

    Konvička, Ondřej; Čížek, Lukáš

    2015-01-01

    Roč. 6, Sept 1 (2015), s. 111-114. ISSN 1804-2732 R&D Projects: GA ČR GAP504/12/1952 Institutional support: RVO:60077344 Keywords : Coleoptera * distributional maps * faunistics Subject RIV: EH - Ecology, Behaviour

  17. Efecto de la dieta artificial MP sobre la emergencia y relacion de sexos de Phymastichus coffea (Hymenoptera:Eulophidae) mantenido sobre su hueped, Hypothenemus hampei (Coleoptera: Scloytidae)a traves de generaciones contin

    Science.gov (United States)

    Phymastichus coffea La Salle (Hymenoptera: Eulophidae) is an endoparasitoid that attacks the adult coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Scolytidae). The MP diet developed by Portilla and Streett is the only reported diet that allows cultures of P. coffea to develop and repr...

  18. Field parasitism of nontarget weevil species (Coleoptera: Curculionidae) by the introduced biological control agent Microctonus aethiopoides Loan (Hymenoptera: Braconidae) over an altitude gradient.

    Science.gov (United States)

    Barratt, B I P; Ferguson, C M; Bixley, A S; Crook, K E; Barton, D M; Johnstone, P D

    2007-08-01

    The parasitoid, Microctonus aethiopoides Loan (Hymenoptera: Braconidae) was introduced into New Zealand in 1982 to control the alfalfa pest, Sitona discoideus Gyllenhal (Coleoptera: Curculionidae). Studies have shown that a number of nontarget weevil species are attacked in the field by this parasitoid. A field study was carried out to investigate nontarget parasitism by M. aethiopoides over an altitudinal sequence from the target host habitat (alfalfa) into native grassland. Three locations were selected for the study, and at each, the alfalfa growing in the valley floor was sampled annually for parasitism of the target pest that ranged between 17 and 78%. At progressively higher altitudes, three further grassland sites at each location were sampled monthly during spring to autumn for up to 6 yr. Weevil densities were estimated, species identified, and dissections carried out to determine reproductive status and parasitism. Almost 12,000 weevils were collected during the study, which were identified as 36 species in total from the three locations. Eight weevil species were found to be parasitized, including S. discoideus, the target host that was found at all sites. Parasitism of nontarget species was approximately 2% overall but varied with location, site, and season. Substantial nontarget parasitism was found at only one of the locations, with up to 24% parasitism of a native weevil, Nicaeana fraudator Broun (Coleoptera: Curculionidae), recorded. Another species, Irenimus egens (Broun) (Coleoptera: Curculionidae), was also found at this location at similar population densities but was attacked far less by M. aethiopoides. Results are discussed in relation to weevil phenology. PMID:17716473

  19. Aspectos biológicos e técnica de criação do gorgulho-da-goiaba, Conotrachelus psidii marshall (Coleoptera: Curculionidae)

    OpenAIRE

    Fabricio Iglesias Valente; Vera Lúcia Rodrigues Machado Benassi

    2014-01-01

    Considerada praga-chave da cultura da goiaba, Conotrachelus psidii Marshall (Coleoptera: Curculionidae) tem causado grandes prejuízos em vários países produtores desta fruta. A pesquisa objetivou avaliar os parâmetros biológicos da praga, sob condições controladas, através do desenvolvimento de uma técnica de criação que permitiu efetuar observações diárias de pré-pupas e pupas. Constatou-se que o substrato adequado para manutenção dessas fases foi o solo argiloso umedecido com viabilidade de...

  20. Description of the male of Eburella pinima Martins and notes on the geographical distribution of Eburodacrys aenigma Galileo & Martins and Eburodacrys lanei Zajciw (Coleoptera, Cerambycidae

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    Juan Pablo Botero

    2013-12-01

    Full Text Available Description of the male of Eburella pinima Martins and notes on the geographical distribution of Eburodacrys aenigma Galileo & Martins and Eburodacrys lanei Zajciw (Coleoptera, Cerambycidae. The male of Eburella pinima Martins, 1997 is described and illustrated for the first time. Information on Eburodacrys aenigma Galileo & Martins, 2006, previously known only from the female holotype, which lacked locality label, is herein complemented. This species is recorded from Brazil and the male is depicted for the first time. The geographical distribution of Eburodacrys lanei Zajciw, 1958 is further restricted here as some previous records are confirmed to result from misidentifications of E. aenigma.

  1. The infestation by an exotic ambrosia beetle, Euplatypus parallelus (F. (Coleoptera: Curculionidae: Platypodinae of Angsana trees (Pterocarpus indicus Willd. in southern Thailand

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    Sara Bumrungsri

    2008-07-01

    Full Text Available An exotic ambrosia beetle, Euplatypus parallelus (F. was collected from infested Pterocarpus indicus Willd. trees in Prince of Songkla University. Larvae and eggs were found in simple galleries with a single branch. Either a single male or a male and a female were found in each gallery. Half of these infested trees were previously attacked by long-horned beetles probably Aristobia horridula (Hope (Coleoptera: Cerambycidae, while some of them appeared to be healthy. Fusarium oxysporum Schlecht.:Fr. was isolated from frass, sapwood samples and insect larvae, and might be a cause of death of P.indicus.

  2. A new species of endogean, anophthalmous Parazuphium Jeannel from Northern Morocco (Coleoptera: Carabidae, with new molecular data for the tribe Zuphiini

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    Carmelo Andújar

    2011-06-01

    Full Text Available A new species of the genus Parazuphium (Coleoptera, Carabidae, Zuphiini, Parazuphium aguilerai sp.n., is described from the Tingitan peninsula in North Morocco. The single known specimen was found below a large stone deeply inserted in the substratum, and it is anophthalmous, depigmented and flattened. This is the second species of blind Parazuphium, the other being P. feloi Machado 1998 from a lave tube in the Canary Islands. Molecular data of the unique known P. aguilerai sp.n. specimen is provided, and a molecular phylogeny confirm its inclusion inside Zuphiini within Harpalinae. Identification keys to the Mediterranean and Macaronesian species of Parazuphium are provided.

  3. Estudio taxonómico de las especies de la familia bruchidae (Insecta: Coleoptera) relacionadas con el género Desmodium (Fabaceae) en el mundo.

    OpenAIRE

    Vásquez Chan, Adriano

    2011-01-01

    La presente investigación se realizó con el propósito de determinar las especies de brúquidos (Coleoptera: Bruchidae) que se alimentan de semillas de plantas del género Desmodium Desv. (Leguminosae: Papilionoideae) en el mundo. Se desarrollaron claves dicotómicas para determinar géneros y especies de brúquidos del Nuevo y Viejo Mundo, además de ampliar la información existente sobre la asociación brúquido-Desmodium de la base de datos BRUCOL. Se revisaron semillas de Desmodium infestadas por ...

  4. A new species of bromeliad-feeding Cephaloleia Chevrolat (Coleoptera, Chrysomelidae, Cassidinae from Costa Rica: evidence from DNA barcodes, larval and adult morphology and insect diets

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    Carlos Garcia-Robledo

    2015-01-01

    Full Text Available The Neotropical genus Cephaloleia Chevrolat (Coleoptera: Chrysomelidae: Cassidinae includes 214 species distributed from the south of Mexico to Argentina. Cephaloleia beetles feed mostly on plants from the order Zingiberales. The interactions between Cephaloleia beetles and their Zingiberales host plants is proposed as one of the oldest and most conservative associations. Here we describe a new species of Cephaloleia (C. kuprewiczae sp. n. that feeds on two species of bromeliads (Pitcairnia arcuata and P. brittoniana, Bromeliaceae: Pitcairnioideae. Cephaloleia kuprewiczae was previously described as Cephaloleia histrionica. This study includes evidence from DNA barcodes (COI, larval and adult morphology and insect diets that separates C. kuprewiczae from C. histrionica as a new species.

  5. Efeitos da radiação gama do Cobalto-60 em ovos de Tribolium castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae Gamma radiation effects on eggs of Tribolium castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae

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    L. S. Fontes

    1994-12-01

    Full Text Available Este trabalho teve por objetivo determinar as doses esterilizantes e letal para ovos de T. castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae, através de doses crescentes de radiação gama. Utilizou-se uma fonte de Cobalto-60, tipo Gammabeam-650, com taxa de dose de 1,28 kGy/hora. O experimento foi conduzido sob condições controladas com temperatura de 25 ± 2°C e umidade relativa de 70 ± 5%. As doses letal e esterilizante para os ovos foram respectivamente 30 e 20 Gy.The objective of this research was to verify the effects of gamma radiation of a Cobalt-60 source on eggs of the red flour beetle Tribolium castaneum (Herbst., 1797. The used dose rate was 1.28 kGy per hour, and the irradiated insects were kept under controlled environment condition: 25 ± 2°C and 70 ± 5% relative humidity. For eggs the sterilizing and lethal dose were respectively, 20 and 30 Gy.

  6. Los ejemplares tipo de Dryopidae, Elmidae, Limnichidae y Psephenidae (Coleoptera: Byrrhoidea, depositados en la colección del Museo de La Plata The types of Dryopidae, Elmidae, Limnichidae and Psephenidae (Coleoptera: Byrrhoidea housed at the Museo de La Plata

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    Liliana A. Fernández

    2004-07-01

    Full Text Available Se examinaron los ejemplares tipo depositados en la colección de la División Entomología del Museo de La Plata, pertenecientes a diversas especies nominales de Byrrhoidea: Dryopidae (1 especie, 1 ejemplar tipo, Elmidae (4, 9, Limnichidae (29, 47 y Psephenidae (1, 2. El material tipo fue estudiado por los siguientes autores: Pic, Spangler, Spangler & Brown, Spangler & Perkins, Spangler & Santiago, Spilman y Wooldridge. Para cada taxón se indica la publicación original, datos de colección y condición del material.The types of species of Byrrhoidea (Coleoptera housed in the collection of the División Entomología of Museo de La Plata were examined and listed. They belong to 35 species distributed in the families Dryopidae (1 species, 1 type, Elmidae (4, 9, Limnichidae (29, 47 and Psephenidae (1, 2. The types were described by Pic, Spangler, Spangler & Brown, Spangler & Perkins, Spangler & Santiago, Spilman, and Wooldridge. For each taxon, complete information about categories of types, reference original descriptions, collection data and specimen condition are given.

  7. Causal agent of tobacco gray mould in Shaanxi Province and preventive effects of Trametes versicolor polysaccharides on the disease%陕西省烟草灰霉病病原及云芝多糖对其防治研究

    Institute of Scientific and Technical Information of China (English)

    李金岭; 罗晶; 单宏英; 成巨龙; 陈德鑫; 安德荣

    2013-01-01

    Based on morphological characters and molecular data, the pathogenic fungus of an unknown tobacco disease in Shaanxi Province in 2011 was identified. The suspected pathogen was isolated from diseased leaves of tobacco plants, and after pathogenicity tests and re-isolation of the pathogen, the strain LJL007 was determined to be responsible for the disease. The strain LJL007 was identified as Botrytis cinerea, whose teleomorph is Botryotinia fuckeliana on basis of morphological and cultural characters. We found that the sequences of the isolate LJL007 (Accession no.: HM17900) was 100% identical to that of B. fuckeliana (Accession no.: HM849615) by analyzing sequences of the ITS regions of ribosomal DNA, further confirming that the pathogen was Botrytis cinerea. Trametes versicolor polysaccharides cannot inhibit the mycelium growth and spore germination of Botrytis cinerea on potato dextrose agar. However, the polysaccharides had relatively good preventive effects on tobacco gray mould in the field, preventive effectiveness was 56.29%. The results of enzyme activity tests showed that Trametes versicolor polysaccharides significantly enhanced activities of chitinase and P-l,3-glucanase in the tobacco plants after treatment, the peak values of them increased by 56.89% and 429.83% as compared to the control treatment (treatment A: only spraying stilled water on the tobacco) respectively. These results indicated that Trametes versicolor polysaccharides could induce and improve resistance of tobacco plants to gray mould.%采用形态学观察和分子鉴定方法对2011年在陕西省发生的一种烟草未知病害的病原菌进行鉴定.从病叶组织分离纯化得到病原菌,通过致病性测定以及人工接种后再分离病菌,证明编号LJL007的菌株为该病的致病菌.依据病原菌的形态学和培养特征,将菌株LJL007鉴定为灰葡萄孢Botrytis cinerea Pers.,其有性型为富氏葡萄孢盘菌Botryotinia fuckeliana Whetzel

  8. Occurrence of adults and biological aspects of Geniates borelli Camerano (Coleoptera, Scarabaeidae, Rutelinae in Aquidauana, MS, Brazil

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    Sergio Roberto Rodrigues

    2012-09-01

    Full Text Available Occurrence of adults and biological aspects of Geniates borelli Camerano (Coleoptera, Scarabaeidae, Rutelinae in Aquidauana, Mato Grosso do Sul, Brazil. Due to the importance of Geniates borelli Camerano as a pest in many crops, studies were developed at Universidade Estadual de Mato Grosso do Sul, Aquidauana campus, MS, Brazil. Adults were collected with a light trap from January 2006 to December 2007. 3,320 adults were collected, and the highest quantities were obtained in October 2006 and November 2007, with 1,548 and 802 adults recorded, respectively. Collected adults were kept in plastic containers with soil and Brachiaria decumbens seedlings for oviposition. 535 eggs measuring 2.30 × 1.60 mm were obtained. As the embryonic development progressed, eggs increased in size to 3.00 × 2.70 mm, and this change occurred between 6 and 10 days after oviposition. The embryonic period lasted 17.9 days. The first, second, and third instars lasted 37.6, 49.7, and 74 days, respectively. The prepupal stage lasted 65.9 days and the pupal stage lasted an average of 18.5 days. The biological cycle is completed in 315.8 days, which characterizes the species as univoltine. The average longevity of females was 35.4 days and 28.5 days for males.

  9. Molecular phylogeny of Diabrotica beetles (Coleoptera: Chrysomelidae) inferred from analysis of combined mitochondrial and nuclear DNA sequences.

    Science.gov (United States)

    Clark, T L; Meinke, L J; Foster, J E

    2001-08-01

    The phylogenetic relationships of thirteen Diabrotica (representing virgifera and fucata species groups) and two outgroup Acalymma beetle species (Coleoptera: Chrysomelidae) were inferred from the phylogenetic analysis of a combined data set of 1323 bp of mitochondrial DNA (mtDNA) cytochrome oxidase subunit 1 (COI) and the entire second internal transcribed spacer region (ITS-2) of nuclear ribosomal DNA of 362 characters. Species investigated were D. adelpha, D. balteata, D. barberi, D. cristata, D. lemniscata, D. longicornis, D. porracea, D. speciosa, D. undecimpunctata howardi, D. u. undecimpunctata, D. virgifera virgifera, D. v. zeae, D. viridula, and outgroup A. blandulum and A. vittatum. Maximum parsimony (MP), minimum evolution (ME), and maximum likelihood (ML) analyses of combined COI and ITS-2 sequences clearly place species into their traditional morphological species groups with MP and ME analyses resulting in identical topologies. Results generally confer with a prior work based on allozyme data, but within the virgifera species group, D. barberi and D. longicornis strongly resolve as sister taxa as well as monophyletic with the neotropical species, D. viridula, D. cristata and D. lemniscata also resolve as sister taxa. Both relationships are not in congruence with the prior allozyme-based hypothesis. Within the fucata species group, D. speciosa and D. balteata resolve as sister taxa. Results also strongly supported the D. virgifera and D. undecimpunctata subspecies complexes. Our proposed phylogeny provides some insight into current hypotheses regarding distribution status and evolution of various life history traits for Diabrotica. PMID:11520353

  10. Updated distribution of Osmoderma eremita in Abruzzo (Italy and agro-pastoral practices affecting its conservation (Coleoptera: Scarabaeidae

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    Patrizia Giangregorio

    2015-12-01

    Full Text Available New records of Osmoderma eremita (Scopoli, 1763 (Coleoptera: Scarabaeidae: Cetoniinae are reported for Abruzzo (Italy, together with a review of its distribution in this region. O. eremita is a saproxylic beetle dependent on the presence of hollow deciduous trees with abundant wood mould in their cavities. The major threats for the species are habitat loss and fragmentation. EU Habitats Directive requests to the member States its protection and the monitoring of its conservation status. Detection of its occurrence is the first step to protect the species. The surveys have been carried out in ten sites of Abruzzo by using black cross-windows traps baited with specific pheromone. The species has been recorded for the first time in the Sant’Antonio forest and its presence is confirmed in the Peligna Valley, after a decade. The populations seem to be confined to small patches of suitable habitats. At local level, the abandonment of the pollarding practice (willow and beech forests and the use of pollarded trees as biomass for fuel are the major threats for this species. Indeed some key actions, such as the protection of old hollow trees and the continuation of pollarding practice in rural landscape, could be key factors for the conservation strategies of the species in the study area.

  11. Population genetics and phylogenetic relationships of beetles (Coleoptera: Histeridae and Staphylinidae) from the Sonoran Desert associated with rotting columnar cacti.

    Science.gov (United States)

    Pfeiler, Edward; Johnson, Sarah; Richmond, Maxi Polihronakis; Markow, Therese A

    2013-12-01

    Dozens of arthropod species are known to feed and breed in the necrotic tissues (rots) of columnar cacti in the Sonoran Desert. Because the necrotic patches are ephemeral, the associated arthropods must continually disperse to new cacti and therefore the populations of any given species are expected to show very little local genetic differentiation. While this has been found to be true for the cactophilic Drosophila, the evolutionary histories and characteristics of other arthropods inhabiting the same necrotic patches, especially the beetles, have yet to be examined. Here we used nucleotide sequence data from segments of the mitochondrial 16S rRNA and cytochrome c oxidase subunit I (COI) genes to examine population structure and demographic history of three sympatric beetle species (Coleoptera: Histeridae and Staphylinidae) collected on senita cactus (Lophocereus schottii) from six widely-separated localities on the Baja California peninsula of northwestern Mexico. Two histerids, Iliotona beyeri and Carcinops gilensis, and an unidentified staphylinid, Belonuchus sp., showed little or no population structure over a broad geographic area on the peninsula, consistent with the prediction that these beetles should show high dispersal ability. Demographic tests revealed varying levels of historical population expansion among the beetle species analyzed, which are discussed in light of their ecologies and concurrent biogeographic events. Additionally, phylogenetic analyses of COI sequences in Carcinops collected on a variety of columnar cacti from both peninsular and mainland Mexico localities revealed several species-level partitions, including a putative undescribed peninsular species that occurred sympatrically with C. gilensis on senita. PMID:23948866

  12. Incidence of turf-damaging white grubs (Coleoptera: Scarabaeidae) and associated pathogens and parasitoids on Kentucky golf courses.

    Science.gov (United States)

    Redmond, Carl T; Potter, Daniel A

    2010-12-01

    Root-feeding grubs (Coleoptera: Scarabaeidae) were sampled from damaged areas of 61 irrigated roughs on 32 Kentucky golf courses to determine species composition and natural enemy incidence, the first such survey in the United States' transitional turfgrass climatic zone. Masked chafers (Cyclocephala lurida Bland and C. borealis Arrow) and Japanese beetle (Popillia japonica Newman) accounted for ≈73 and 26% of grubs found in an autumn survey, with Cyclocephala spp. predominating at most sites, although mixed infestations were common. Only a few Phyllophaga spp., and no exotic species other than P. japonica were found. Cyclocephala spp. also predominated in seasonal and statewide surveys regardless of whether a course had cool- or warm-season grass fairways. Pathogenic bacteria, Paenibacillus and Serratia spp., and the autumn-active parasitoid Tiphia pygidialis Allen were the main enemies associated with Cyclocephala spp. Predominant enemies of P. japonica were Paenibacillus, Serratia, and Metarhizium spp. in autumn, and eugregarines, Stictospora sp. (probably S. villani Hays and Clopton) and Tiphia vernalis Rohwer in spring. Entomopathogenic nematodes and the microsporidian Ovavesicula popilliae Andreadis & Hanula were nearly absent in our samples. No predictive relationships were found between soil parameters and proportionate abundance of Cyclocephala or P. japonica, or with natural enemy incidence at particular sites. Although incidence of individual enemies was generally low (hosts' prolonged development they may take a significant toll on grub populations. PMID:22182549

  13. Attraction of Tomicus yunnanensis (Coleoptera: Scolytidae to Yunnan Pine Logs with and without Periderm or Phloem: An Effective Monitoring Bait

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    Rong Chun Lu

    2012-01-01

    Full Text Available The Yunnan pine shoot beetle, Tomicus yunnanensis Kirkendall and Faccoli (Coleoptera: Scolytinae is an important pest of Yunnan pine (Pinus yunnanensis Franch in China. Experiments with host log baits were done to develop a pest monitoring system using host tree kairomone. Five Yunnan pine logs (each 10–15 cm diam. × 30-cm long in a trap-log bundle were treated by peeling periderm (outer bark off to expose the phloem, and half of each log was covered with sticky adhesive to capture any attracted adult beetles. Significantly, more beetles were attracted and caught on the periderm-peeled logs (ca 30 beetles/m2 log surface/day than on untreated control logs with adhesive (ca 2.5/m2/day. No significant differences were observed between catches on logs taken from lower or upper halves of Yunnan pines. T. yunnanensis flies mostly during the afternoon according to trap catches throughout the day. Attraction to the periderm-peeled logs decreased considerably when they were peeled further to remove the phloem, indicating phloem volatiles play a role in selection of the host by the beetle. The readily-available log baits appear useful for monitoring pine shoot beetle populations in integrated pest management programs.

  14. Morfología e histología del sistema digestivo del burrito del frambueso Aegorhinus superciliosus (Coleoptera: Curculionidae

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    VIVIAN MEDEL

    2013-12-01

    Full Text Available Se realizó el estudio morfológico e histológico del sistema digestivo de Aegorhinus superciliosus (Coleoptera: Curculionidae, plaga de importancia económica en el sur de Chile. Esta especie presenta un sistema digestivo característico para la mayoría de los insectos, siendo similar en machos y hembras, pero de mayor longitud en las últimas, llegando a medir el doble del tamaño del insecto. El sistema digestivo es un tubo largo de diámetro variable, dividido en tres regiones: intestino anterior (estomodeo, intestino medio (mesenterón e intestino posterior (proctodeo. Histológicamente presenta un tejido epitelial similar al descrito para otras especies y externamente un tejido muscular, sin diferencias entre machos y hembras. No obstante, presenta características que difieren en otros curculiónidos y coleópteros tales como: ausencia de buche, ubicación y número de los ciegos gástricos, presencia de membrana peritrófica y ausencia de almohadillas rectales.

  15. Occurrence and biology of Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae) parasitising different species of Coccinellidae (Coleoptera) in Neotropical region.

    Science.gov (United States)

    Silva, R B; Cruz, I; Figueiredo, M L C; Pereira, A G; Penteado-Dias, A M

    2012-02-01

    Surveys on Coccinellidae (Coleoptera) in Sete Lagoas city, Minas Gerais state, Brazil, indicated the parasitism of adults of the species Coleomegilla maculata De Geer, 1775, Eriopis connexa (Germar, 1824) and Olla v-nigrum (Mulsant, 1866), by Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae). Since then, the parasitoid have been maintained in its original hosts at the Insect Rearing Laboratory - LACRI of the Brazilian Agricultural Research Institution - Embrapa Milho e Sorgo. Besides the citation of occurrence in Brazil, this work also indicates the parasitoid preference for C. maculata (70% of parasitism), followed by O. v-nigrum (43.3% of parasitism) and E. connexa (36.7% of parasitism). Total life cycle of D. coccinellae was longer on C. maculata (32.4 ± 0.48 days), compared to O. v-nigrum (29.5 ± 0.49 days) and E. connexa (27.8 ± 0.4 days). Due to the relatively high percentage of field parasitism, D. coccinellae can reduce the efficiency of biological pest control by Coccinellidae predators especially in the case of C. maculata. PMID:22437405

  16. Coleoptera families other than Cerambycidae, Curculionidae sensu lato, Chrysomelidae sensu lato and Coccinelidae. Chapter 8.5

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    Olivier Denux

    2010-07-01

    Full Text Available Here we consider 274 alien Coleoptera species belonging to 41 of the 137 beetle families in Europe (Cerambycidae, Curculionidae sensu lato, Chrysomelidae sensu lato and Coccinelidae are treated separately elsewhere. Among the families we consider asinvaded the European fauna, Acanthocnemidae and Ptilodactylidae represent new arrivals. Many species-rich families have surprisingly few aliens, whereas some relatively minor families such as Dermestidae, Nitidulidae and Anobiidae have a relatively high representation of alien species. Since the start of the 19th century, the number of coleopteran aliens introduced into Europe has continued to increase. Alien species colonizing Europe derive from a wide range of geographic regions as well as ecozones, but the most important source area is Asia. The countries with the largest number of alien species established are France, Germany and Italy. The majority have been introduced accidentally via international transport mechanisms. The most important route for importation is stored products and crops, followed by transport of wood, then horticultural and ornamental plants. Most alien species in these families are found within anthropogenic habitats in Europe. The introduction of invasive alien beetles in these families has had significant economic impacts, particularly as pests of stored foodstuffs, as well as serious ecological impacts. For example, the buprestid species Agrilus planipennis, recently recorded in Russia, is an important potential economic threat which may also impact the biodiversity associated with ash trees.

  17. Inheritance and Fitness Costs of Resistance to Cry3Bb1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Ingber, David A; Gassmann, Aaron J

    2015-10-01

    Transgenic crops that produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are widely planted to manage pest insects. One of the primary pests targeted by Bt corn in the United States is western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae). Cry3Bb1 corn for management of western corn rootworm was commercialized in 2003, and beginning in 2009, populations of western corn rootworm with field-evolved resistance to Cry3Bb1 corn were found in Iowa. Here we quantify the magnitude, inheritance, and fitness costs of resistance to Cry3Bb1 corn in two strains (Hopkinton and Cresco) derived from field populations that evolved resistance to Cry3Bb1 corn. For Hopkinton, we found evidence for complete resistance to Cry3Bb1 corn and nonrecessive inheritance. Additionally, no fitness costs of Cry3Bb1 resistance were detected for Hopkinton. For Cresco, resistance was incomplete and recessive, and we detected fitness costs affecting developmental rate, survival to adulthood, and fecundity. These results suggest that variation may exist among field populations in both the inheritance and accompanying fitness costs of resistance. To the extent that field populations exhibit nonrecessive inheritance and a lack of fitness cost, this will favor more rapid evolution of resistance than would be expected when resistance is functionally recessive and is accompanied by fitness costs. PMID:26453731

  18. A DNA barcode library of the beetle reference collection (Insecta: Coleoptera in the National Science Museum, Korea

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    Sang Woo Jung

    2016-06-01

    Full Text Available Coleoptera is a group of insects that are most diverse among insect resources. Although used as indicator species and applied in developing new drugs, it is difficult to identify them quickly. Since the development of a method using mitochondrial DNA information for identification, studies have been conducted in Korea to swiftly and accurately identify species. The National Science Museum of Korea (NSMK has been collecting and morphologically identifying domestic reference insects since 2013, and building a database of DNA barcodes with digital images. The NSMK completed construction of a database of digital images and DNA barcodes of 60 beetle species in the Korean National Research Information System. A total of 179 specimens and 60 species were used for the analysis, and the averages of intraspecific and interspecific variations were 0.70±0.45% and 26.34±6.01%, respectively, with variation rates ranging from 0% to 1.45% and 9.83% to 56.23%, respectively.

  19. Revision of the Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera, Dytiscidae, Hydroporinae, Bidessini)

    Science.gov (United States)

    Miller, Kelly B.

    2016-01-01

    Abstract The Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera: Dytiscidae: Hydroporinae: Bidessini) is revised. Thirty species are recognized. The following new species are described: Hydrodessus bimaculatus sp. n. (Venezuela), Hydrodessus brevis sp. n. (Venezuela), Hydrodessus concolorans sp. n. (Venezuela), Hydrodessus continuus sp. n. (Venezuela), Hydrodessus disjunctus sp. n. (Suriname), Hydrodessus fasciatus sp. n. (Brazil), Hydrodessus imparilis sp. n. (Ecuador), Hydrodessus keithi sp. n. (Brazil, Colombia, Ecuador), Hydrodessus kurti sp. n. (Suriname), Hydrodessus kylei sp. n. (Suriname, Venezuela), Hydrodessus laetus sp. n. (Venezuela), Hydrodessus latotibialis sp. n. (Peru), Hydrodessus maculatus sp. n. (Guyana, Venezuela), Hydrodessus morsus sp. n. (Venezuela), Hydrodessus palus sp. n. (Venezuela), and Hydrodessus tenuatus sp. n. (Suriname). The following new synonyms are established: Hydrodessus fragrans Spangler, 1985 = Hydrodessus biguttatus (Guignot, 1957) syn. n. and Hydrodessus robinae Spangler, 1985 = Hydrodessus octospilus (Guignot, 1957), syn. n. One species is transferred from Hydrodessus to Amarodytes Régimbart, Amarodytes soekhnandanae (Makhan, 1994), comb. n. Habitus photographs (dorsal and lateral) and photos of the ventral surfaces are provided for most species. Line drawings of male and female genitalia and other diagnostic features are also provided along with distribution maps.

  20. Effects of irradiated diet on longevity and prolificity of successive generations of Zabrotes subfasciatus (Boheman, 1833) (Coleoptera, Bruchidae)

    International Nuclear Information System (INIS)

    The study was done in the laboratory of Entomology of the Centro de Energia Nuclear na Agricultura (CENA), University of Sao Paulo, in Piracicaba, State of Sao Paulo, Brazil. This research was carried out to observe the effect of irradiated beans (Phaseolus vulgaris, (L.), cultivar Rosinha) on the longevity and prolificity of Zabrotes subfasciatus (Boheman, 1833) (Coleoptera, Bruchidae). The first part of the study included the rearing of five generations of Zabrotes subfasciatus which were fed with irradiated beans (0, 10, 20, 100 e 200 krad, with a dose rate of approximately 70 krad/h). The second one consisted of growth of seven generations on irradiated beans (0, 100, 500, 1500 and 3000 krad, with a dose rate of approximately 70 krad/h). The data were obtained through daily countings of insect birth and death rates. It was concluded that irradiation modified the nutritional quality of beans, affecting the development of Zabrotes subfasciatus. This effect was studied through the longevity and prolificity, which were altered as compared with the control, once they were higher for the dosis of 1500 and 3000 krad. (author)

  1. Effects of seed type and bruchid genotype on the performance and oviposition behavior of Zabrotes subfasciatus (Coleoptera: Bruchidae)

    Institute of Scientific and Technical Information of China (English)

    ERICK D. M. CAMPAN; BETTY BENREY

    2006-01-01

    The effect of different bean varieties on the performance of the bruchid beetle Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae), was determined by using wild and cultivated seeds of the genus Phaseolus. Results showed that the quality of the host plant affected the performance and the oviposition behavior of female beetles. Overall, bruchid performance was higher on cultivated seeds than on wild seeds. It was also found that the oviposition behavior and the performance of their offspring differed between females that originated from wild versus cultivated seeds. We also demonstrated the importance of a genetic component in bruchid performance: longevity, fecundity, larval development, adult For example, on the same host type, some females laid twice as many eggs as females from Thus, the performance and behavior of Z. subfasciatus are not only affected by environmental factors such as the quality of the seeds on which they develop, but also have a genetic basis which can counterbalance a less suitable quality of the host plant. For a crop pest such as Z.subfasciatus, its ability to survive and adapt on host plants of differing quality may be an important attribute to consider for pest management.

  2. Knockdown, residual, and antifeedant activity of pyrethroids and home landscape bioinsecticides against Japanese beetles (Coleoptera: Scarabaeidae) on Linden foliage.

    Science.gov (United States)

    Baumler, Rebecca E; Potter, Daniel A

    2007-04-01

    Residual toxicity and leaf protection capability of five pyrethroids, professional and home garden azadirachtin formulations, and six other bioinsecticides for the home landscape were evaluated against the Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), on linden, Tilia cordata L. Capacity of intoxicated beetles to recover and subsequently feed and disperse also was evaluated to provide insight on activity characteristics of the different compounds. Intact shoots were sprayed and left in the field for varying intervals before being challenged with beetles in no-choice and choice tests. All pyrethroids except permethrin gave greater leaf protection, knockdown, and kill than did carbaryl, the standard, after 14 d of weathering. Deltamethrin, cyfluthrin, bifenthrin, and lamda-cyhalothrin gave a high level of protection for at least 19 d, and azadirachtin (Azatin XL) deterred feeding in choice tests for as long as 14 d. Home garden formulations containing pyrethrins in canola oil (Pyola) or azadiractin (Neem-Away) gave good short-term (< 3-d) protection. Formulations of capsaicin, rotenone + pyrethrins, kaolin particle film, D-limonene, or garlic extract were ineffective, the latter two formulations being highly phytotoxic to linden. Results of this study should help support updating of guidelines for insecticidal control of Japanese beetles. PMID:17461070

  3. Biology and host preferences of Cryptorhynchus melastomae (Coleoptera: Curculionidae), a possible biocontrol agent for Miconia calvescens (Melastomataceae) in Hawaii.

    Science.gov (United States)

    Reichert, Elisabeth; Johnson, M Tracy; Chacón, Eduardo; Anderson, Robert S; Wheeler, Terry A

    2010-12-01

    The introduced plant Miconia calvescens (Melastomataceae) poses a grave threat to Hawaii's native ecosystems and biodiversity. One potential candidate for classical biological control is Cryptorhynchus melastomae (Coleoptera: Curculionidae: Cryptorhynchinae), a stem-boring weevil from Central and South America. This weevil feeds on M. calvescens in its native Costa Rica and has been successfully reared under greenhouse conditions. Comparison of its environmental conditions in Costa Rica with those in the Miconia infested areas of Hawaii indicates the latter is a suitable habitat for C. melastomae. C. melastomae has one or two generations per year. Adults feed on new stems, petioles, leaf buds, veins, and lamina, whereas larvae mine the stem until pupation. Adults appear to prefer saplings for oviposition and feeding. Under greenhouse conditions both adults and larvae can seriously damage and kill small M. calvescens. Preliminary host testing indicates that C. melastomae may be family specific on Melastomataceae. However, because Hawaii lacks native melastomes and has many other serious melastome weeds, a family specific insect may be suitable as a biocontrol agent in this case. PMID:22182550

  4. Acute toxicity of plant essential oils to scarab larvae (Coleoptera: Scarabaeidae) and their analysis by gas chromatography-mass spectrometry.

    Science.gov (United States)

    Ranger, Christopher M; Reding, Michael E; Oliver, Jason B; Moyseenko, James J; Youssef, Nadeer; Krause, Charles R

    2013-02-01

    Larvae of scarab beetles (Coleoptera: Scarabaeidae) are important contaminant and root-herbivore pests of ornamental crops. To develop alternatives to conventional insecticides, 24 plant-based essential oils were tested for their acute toxicity against third instars of the Japanese beetle Popillia japonica Newman, European chafer Rhizotrogus majalis (Razoumowsky), oriental beetle Anomala orientalis (Waterhouse), and northern masked chafer Cyclocephala borealis Arrow. Diluted solutions were topically applied to the thorax, which allowed for calculating LD50 and LD90 values associated with 1 d after treatment. A wide range in acute toxicity was observed across all four scarab species. Of the 24 oils tested, allyl isothiocyanate, cinnamon leaf, clove, garlic, and red thyme oils exhibited toxicity to all four species. Allyl isothiocyanate was the most toxic oil tested against the European chafer, and among the most toxic against the Japanese beetle, oriental beetle, and northern masked chafer. Red thyme was also comparatively toxic to the Japanese beetle, oriental beetle, European chafer, and northern masked chafer. Interspecific variability in susceptibility to the essential oils was documented, with 12, 11, 8, and 6 of the 24 essential oils being toxic to the oriental beetle, Japanese beetle, European chafer, and northern masked chafer, respectively. Analysis of the active oils by gas chromatography-mass spectrometry revealed a diverse array of compounds, mostly consisting of mono- and sesquiterpenes. These results will aid in identifying active oils and their constituents for optimizing the development of plant essential oil mixtures for use against scarab larvae. PMID:23448028

  5. Revision of the Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera, Dytiscidae, Hydroporinae, Bidessini).

    Science.gov (United States)

    Miller, Kelly B

    2016-01-01

    The Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera: Dytiscidae: Hydroporinae: Bidessini) is revised. Thirty species are recognized. The following new species are described: Hydrodessus bimaculatus sp. n. (Venezuela), Hydrodessus brevis sp. n. (Venezuela), Hydrodessus concolorans sp. n. (Venezuela), Hydrodessus continuus sp. n. (Venezuela), Hydrodessus disjunctus sp. n. (Suriname), Hydrodessus fasciatus sp. n. (Brazil), Hydrodessus imparilis sp. n. (Ecuador), Hydrodessus keithi sp. n. (Brazil, Colombia, Ecuador), Hydrodessus kurti sp. n. (Suriname), Hydrodessus kylei sp. n. (Suriname, Venezuela), Hydrodessus laetus sp. n. (Venezuela), Hydrodessus latotibialis sp. n. (Peru), Hydrodessus maculatus sp. n. (Guyana, Venezuela), Hydrodessus morsus sp. n. (Venezuela), Hydrodessus palus sp. n. (Venezuela), and Hydrodessus tenuatus sp. n. (Suriname). The following new synonyms are established: Hydrodessus fragrans Spangler, 1985 = Hydrodessus biguttatus (Guignot, 1957) syn. n. and Hydrodessus robinae Spangler, 1985 = Hydrodessus octospilus (Guignot, 1957), syn. n. One species is transferred from Hydrodessus to Amarodytes Régimbart, Amarodytes soekhnandanae (Makhan, 1994), comb. n. Habitus photographs (dorsal and lateral) and photos of the ventral surfaces are provided for most species. Line drawings of male and female genitalia and other diagnostic features are also provided along with distribution maps. PMID:27110208

  6. Developmental Biology and Effects of Adult Diet on Consumption, Longevity, and Fecundity of Colaspis crinicornis (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Miwa, Kentaro; Meinke, Lance J

    2015-01-01

    The chrysomelid beetle Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) occurs primarily in the Great Plains region of the United States. Little is known about the biology and ecology of this species, but over the last decade, it has become increasingly common in the corn, Zea mays L., and soybean, Glycine max (L.) Merrill, agroecosystem of southeastern Nebraska. As part of a larger comprehensive project to understand the natural history and pest potential of this species, laboratory experiments were conducted to study the developmental biology, morphological characters of immature stages, and the effect of adult diet on consumption, longevity, and fecundity. Females readily deposited egg clusters in the soil, and percentage egg hatch was high under laboratory conditions. Larvae and pupae were confirmed to be soil-dwelling stages. C. crinicornis has relatively short egg, pupal, and adult stages with the majority of its life cycle spent in the larval stage. Results of choice and no-choice adult feeding experiments indicate that diets of corn or soybean leaves did not significantly affect consumption, longevity, or fecundity of adult C. crinicornis, suggesting that corn and soybean leaves are similarly suitable food sources for adults. The ability to effectively utilize tissues from very different plant families as adult food sources suggests that C. crinicornis is polyphagous in the field. PMID:26106090

  7. Repellent Activity of the Essential Oil from the Heartwood of Pilgerodendron uviferum (D. Don) Florin against Aegorhinus superciliosus (Coleoptera: Curculionidae).

    Science.gov (United States)

    Espinoza, Javier; Urzúa, Alejandro; Tampe, Jocelyne; Parra, Leonardo; Quiroz, Andrés

    2016-01-01

    The weevil Aegorhinus superciliosus Guérin (Coleoptera: Curculionidae), which is endemic to Central-Southern Chile and Argentina, is one of the major berry pests in Chile and the most important pest in the La Araucanía Region (38°44'9″S, 72°35'25″W). Due to the poor effectiveness and problems surrounding the implementation of the traditional control methods using organophosphate and carbamate insecticides, new strategies for controlling this pest are needed. In this communication, we evaluated the behavioral responses of male and female A. superciliosus to volatile compounds released from the essential oil (EO) obtained from the heartwood of Pilgerodendron uviferum (D. Don) Florin using olfactometric bioassays. The composition of the EO was analyzed using gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). According to these analyses, δ-cadinol (24.16%), cubenol (22.64%), 15-copaenol (15.46%) and δ-cadinene (10.81%) were the principal components of the EO. The Pilgerodendron uviferum EO, which is almost exclusively composed of sesquiterpenes (99.5%), exhibited a repellent effect against A. superciliosus adults, regardless of the sex or concentration used (56.6 mg/cm³ and 1.58 × 10(-2) mg/cm³). The EO has low volatility and greater persistence than the EOs composed of monoterpenes and is considered a good model in the search for raspberry weevil repellents. PMID:27110756

  8. ESTUDIO DEL EFECTO DE DOS INDUCTORES Y UN PROTECTOR ENZIMÁTICO SOBRE LA ACTIVIDAD DE LAS ENZIMAS Mn-PEROXIDASA Y LACASA PRODUCIDAS POR Trametes versicolor Y SU EFECTO EN LA DECOLORA- CIÓN DE EFLUENTES DE LA INDUSTRIA PAPELERA

    Directory of Open Access Journals (Sweden)

    C. Gómez-Dorado

    2005-12-01

    Full Text Available En este estudio se realizaron dos factoriales 2 3 para determinar el efecto del Tween80, como protector enzimático y la adición de MnSO4 y CuSO4 como inductores enzimáticos del hongo Trametes versicolor sobre un efluente estéril y uno no estéril. Para tal fin se realizó un diseño factorial 2 3 con tres concentraciones de cada uno de los inductores además del protector enzimático, siendo éstos las variables independientes; las variables dependientes del factorial fueron el porcentaje de decoloración y la actividad enzimática las cuales fueron determinadas por métodos colorimétricos, además del pH. Los datos generados en este estudio, determinaron que las diferentes concentraciones de los inductores definidos para cada una de las enzimas, no tienen un efecto significativo sobre la actividad enzimática. Se determinaron máximos valores de actividad para la enzima Mn-peroxidasa de 6.19U y de 18.17U y los valores de actividad de la enzima Lacasa fueron de 4.99U y de 2.37U en los factoriales estéril y no estéril. Estos datos de actividad no fueron relacionados con los máximos índices de decoloración que fueron del 85% y 61% en cada uno de los factoriales al adicionar 0.02% de Tween80, 2mM de MnSO 4 y 5ppm de CuSO4. Las diferencias en la decoloración entre los dos efluentes, es dada por la cantidad de sólidos suspendidos en el medio que cambian su conformación al ser esterilizados y por la acumulación de metabolitos en el medio debido al crecimiento de flora nativa y del mismo hongo Trametes versicolor.

  9. Calliini (Coleoptera, Cerambycidae, Lamiinae: descrições, homonímia, novo registro e chave para as espécies de Graminea Thomson, 1864

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2006-01-01

    Full Text Available Calliini (Coleoptera, Cerambycidae, Lamiinae: descrições, homonímia, novo registro e chave para as espécies de Graminea Thomson, 1864. Espécies novas descritas: Paracallia giesberti sp. nov. da Bolívia (Cochabamba, Colombicallia albofasciata sp. nov. e Graminea rubra sp. nov. da Bolívia (Santa Cruz; Asemolea flava sp. nov. da Costa Rica, (Heredia; Callia potiaiuba sp. nov. do Brasil (Rondônia e da Bolívia (Cochabamba e Santa Cruz. É proposto o nome Rumuara nom. nov. para Paracladus Martins & Galileo, 1990 non Carpenter, 1966 (Orthoptera. Apresenta-se novo registro para a Bolívia para Eumimesis trilineata Magno & Monné, 1990. Acrescenta-se chave para as espécies de Graminea.Calliini (Coleoptera, Cerambycidae, Lamiinae: descriptions, homonym, new record and key to the species of Graminea Thomson,1864. New species described: Paracallia giesberti sp. nov. from Bolivia (Cochabamba, Colombicallia albofasciata sp. nov. and Graminea rubra sp. nov. from Bolivia (Santa Cruz; Asemolea flava sp. nov. from Costa Rica, (Heredia; Callia potiaiuba sp. nov. from Brazil (Rondônia and Bolivia (Cochabamba and Santa Cruz. Rumuara nom. nov. is proposed for Paracladus Martins & Galileo, 1990 non Carpenter, 1966 (Orthoptera. Eumimesis trilineata Magno & Monné, 1990 is recorded for Bolivia. A key to the species of Graminea is added.

  10. Elucidating the Common Generalist Predators of Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae) in an Organic Apple Orchard Using Molecular Gut-Content Analysis.

    Science.gov (United States)

    Schmidt, Jason M; Szendrei, Zsofia; Grieshop, Matthew

    2016-01-01

    Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), plum curculio, is a serious direct pest of North American tree fruit including, apples, cherries, peaches and plums. Historically, organophosphate insecticides were used for control, but this tool is no longer registered for use in tree fruit. In addition, few organically approved insecticides are available for organic pest control and none have proven efficacy as this time. Therefore, promoting biological control in these systems is the next step, however, little is known about the biological control pathways in this system and how these are influenced by current mechanical and cultural practices required in organic systems. We used molecular gut-content analysis for testing field caught predators for feeding on plum curculio. During the study we monitored populations of plum curculio and the predator community in a production organic apple orchard. Predator populations varied over the season and contained a diverse assemblage of spiders and beetles. A total of 8% of all predators (eight Araneae, two Hemiptera, and six Coleoptera species) assayed for plum curculio predation were observed positive for the presence of plum curculio DNA in their guts, indicating that these species fed on plum curculio prior to collection Results indicate a number of biological control agents exist for this pest and this requires further study in relation to cultural practices. PMID:27348005

  11. Molecular cloning and functional characterization of the sex-determination gene doublesex in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Coleoptera, Tenebrionidae)

    Science.gov (United States)

    Gotoh, Hiroki; Ishiguro, Mai; Nishikawa, Hideto; Morita, Shinichi; Okada, Kensuke; Miyatake, Takahisa; Yaginuma, Toshinobu; Niimi, Teruyuki

    2016-01-01

    Various types of weapon traits found in insect order Coleoptera are known as outstanding examples of sexually selected exaggerated characters. It is known that the sex determination gene doublesex (dsx) plays a significant role in sex-specific expression of weapon traits in various beetles belonging to the superfamily Scarabaeoidea. Although sex-specific weapon traits have evolved independently in various Coleopteran groups, developmental mechanisms of sex-specific expression have not been studied outside of the Scarabaeoidea. In order to test the hypothesis that dsx-dependent sex-specific expression of weapon traits is a general mechanism among the Coleoptera, we have characterized the dsx in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Tenebrionidea, Tenebirionidae). By using molecular cloning, we identified five splicing variants of Gnatocerus cornutus dsx (Gcdsx), which are predicted to code four different isoforms. We found one male-specific variant (GcDsx-M), two female-specific variants (GcDsx-FL and GcDsx-FS) and two non-sex-specific variants (correspond to a single isoform, GcDsx-C). Knockdown of all Dsx isoforms resulted in intersex phenotype both in male and female. Also, knockdown of all female-specific isoforms transformed females to intersex phenotype, while did not affect male phenotype. Our results clearly illustrate the important function of Gcdsx in determining sex-specific trait expression in both sexes. PMID:27404087

  12. Notas e descrições em Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. Novos táxons, nova sinonímia e novos registros

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    Ubirajara R. Martins

    2007-01-01

    Full Text Available Notas e descrições em Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. Novos táxons e novos registros. Descrição de espécies novas: Zikanita egregia sp. nov. (Bolívia, Santa Cruz; Dufauxia thomasi sp. nov. (Bolívia, Santa Cruz; Irundisaua punctata sp. nov. (Colômbia, Cauca; Cotyzineus gen. nov., espécie-tipo C. bruchi (Melzer, 1931 comb. nov. Novos registros: Zikanita argenteofasciata (Tippmann, 1960 é assinalada para o Brasil (Maranhão e Cotyzineus bruchi para a Bolívia (Santa Cruz. Novo sinônimo: Ateralphus diringsi Martins & Monné, 1993 = A. javariensis Lane, 1965.Notes and descriptions on Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. New taxa and new records. New taxa described: Zikanita egregia, sp. nov. from Bolivia (Santa Cruz; Dufauxia thomasi sp. nov. from Bolivia (Santa Cruz; Irundisaua punctata sp. nov. from Colombia (Cauca; Cotyzineus gen. nov. type species C. bruchi (Melzer, 1931 comb. nov. New records: Zikanita argenteofasciata (Tippmann, 1960 for Brazil (Maranhão; Cotyzineus bruchi for Bolivia (Santa Cruz. New synomym: Ateralphus diringsi Martins & Monné, 1993 = A. javariensis Lane, 1965.

  13. Aspectos biológicos de Coccidophilus citricola (Coleoptera, Coccinellidae sobre Aspidiotus nerii e Chrysomphalus aonidum (Homoptera, Diaspididae Biological studies of Coccidophilus citricola (Coleoptera, Coccinellidae on Aspidiotus nerii and Chrysomphalus aonidum (Homoptera, Diaspididae

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    Antonio Cesar dos Santos

    2005-02-01

    Full Text Available Aspectos biológicos de Coccidophilus citricola Brèthes (Coleoptera, Coccinellidae foram estudados sobre Chrysomphalus aonidum Linnaeus e Aspidiotus nerii Bouchè (Homoptera, Diaspididae criadas sobre abóbora. Observou-se a duraç��o (dias do período de ovo, dos quatro ínstares, pré-pupa, pupa, longevidade de adultos e a proporção machos: fêmeas de C. citricola. Determinou-se também verificar a eficiência de predação de adultos de C. citricola sobre A. nerii. C. aonidum e A. nerii são presas adequadas para C. citricola, com desenvolvimento semelhante do coccinelídeo sobre essas cochonilhas; o período de incubação do ovo foi de 7,9 e 8,1 dias com A. nerii e C. aonidum; a duração (dias de cada ínstar larval de C. citricola sobre A. nerii e C. aonidum foram respectivamente de: (I 2,7 e 2,9; (II 3,1 e 3,3; (III 3,0 e 3,3 e (IV 3,2 e 3,1 dias. A duração das fases de pré-pupa e pupa foi de 2,5 e 4,5 dias sobre A. nerii e 2,8 e 4,8 dias sobre C. aonidum e a viabilidade de ovo a adulto foi de 63,2% e 62,7% respectivamente para indivíduos criados sobre essas presas. A longevidade de adultos de C. citricola foi de 57,0 dias com A. nerii e de 62,0 dias com C. aonidum. A proporção macho: fêmea foi de 0,82:1,00. O consumo diário de C. citricola foi de 1,15 adultos ou 2,35 ninfas de 2o ínstar ou 3,16 ninfas de 1o ínstar de A. nerii por dia.Laboratory trials were carried out to study biological aspects of Coccidophilus citricola Brèthes (Coleoptera: Coccinellidae on Chrysomphalus aonidum Linnaeus and Aspidiotus nerii Bouché (Homoptera: Diaspididae. The scales were reared on squashes and then served as food source to the predator. The period of egg, larvae (four ínstars, pre-pupae, pupae, adults’ longevity and male: female ratio was observed. Predatory efficacy of C. citricola on A. nerii was also determined. The period of egg was 7.9 and 8.1 days on A. nerii and C. aonidum. The development (days of different stages of C

  14. Viabilidade de sementes de trigo tratadas com fenitrotion e infestadas por Sitophilus oryzae (L. (Coleoptera: Curculionidae durante o armazenamento Viability of stored wheat seeds when treated with fenitrothion and infested by Sitophilus oryzae (L. (Coleoptera: Curculionidae

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    Isabel Helena de Almeida Zeituni Plazas

    2003-01-01

    Full Text Available O esclarecimento de aspectos do comportamento de gorgulhos (Sitophilus spp. em sementes de trigo armazenadas e o acompanhamento da qualidade física e fisiológica das sementes neste contexto, é essencial para aprimorar o monitoramento das sementes durante a armazenagem. Com o objetivo de avaliar a eficiência do inseticida fenitrotion, quanto à proteção oferecida às sementes de trigo contra os insetos Sitophilus oryzae (L. (Coleoptera: Curculionidae e de acompanhar a germinação das sementes tratadas ou não com inseticida, durante o armazenamento, sementes de trigo dos cultivares IAC-120 e IAC-350 foram tratadas ou não com fenitrotion três meses após a colheita e armazenadas em condições de ambiente de laboratório, em Campinas (SP, durante 15 meses. Em intervalos trimestrais, adultos de S. oryzae foram colocados nas amostras de sementes de cada uma das repetições dos tratamentos com insetos. Avaliou-se a sobrevivência desses insetos, bem como a progênie resultante. Em seguida, as sementes das amostras das repetições de todos os tratamentos com e sem insetos foram analisadas quanto ao grau de umidade, germinação e infestação por insetos. O inseticida fenitrotion, na dose recomendada pelo fabricante, não permitiu a proteção das sementes contra a infestação por S. oryzae, mesmo por períodos curtos de dois meses e que, na ausência de insetos, as sementes de trigo com elevado potencial de germinação inicial podem mantê-lo inalterado por períodos de 14 a 17 meses após a colheita, quando armazenadas em condições ambientes, em regiões com clima semelhante ao de Campinas.Clarifying aspects of the weevils (Sitophilus spp. behavior on stored wheat seeds, considering the seed quality, is essential to improve the seed management during storage. In order to evaluate the efficiency of the insecticide fenitrothion as protecting wheat seeds against Sitophilus oryzae (L. (Coleoptera: Curculionidae and to measure the seed

  15. Addenda and Corrigenda to the Catalogue of Palaearctic Coleoptera, volumes 7 and 8 (Curculionoidea

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    Alonso-Zarazaga, Miguel A.

    2016-06-01

    Full Text Available Additions, corrections, comments and nomenclatural novelties for the volumes 7 and 8 of the Catalogue of Palaearctic Coleoptera are provided. For the exact authorship of these check the text. One new species is described: Mecinus tavaresi Caldara & Fogato, sp. nov. from Portugal and Spain. New synonymies are: Compsapoderus (Compsapoderus erythropterus (Gmelin, 1790 = Attelabus intermedius Hellwig, 1795, syn. nov.; Paroplapoderus (Erycapoderus angulipennis (Kolbe, 1886 = Paroplapoderus (Erycapoderus angulipennis shaanxinsis Legalov, 2004, syn. nov. (Attelabidae; Aspidapion (Koestlinia aeneum (Fabricius, 1775 = Aspidapion (Koestlinia motschulskyi (Hochhuth, 1847, syn. nov., Taeniapion rufescens (Gyllenhal, 1833 = Taeniapion notatum (Wagner, 1912, syn. nov. (Apionidae; Larinus (Larinomesius scolymi (Olivier, 1807 = Curculio teres Hellwig, 1795, syn. nov. (Curculionidae; Lixus paraplecticus (Linnaeus, 1758 = Curculio phellandrii Linnaeus, 1764, syn. nov. (Curculionidae; Curculio alternans Hellwig, 1795= Curculio alternans Herbst, 1795, syn. nov. (Curculionidae; Sibinia lyrata Faust, 1889= Sibinia attalica var. judea Pic, 1901, syn. nov. (Curculionidae; Phyllobius (Metaphyllobius pomaceus Gyllenhal, 1834= Curculio prasinus Olivier, 1791, syn. nov. (Curculionidae. New homonymies are: Attelabus intermedius Hellwig, 1795 (non Attelabus intermedius Illiger, 1794; Baris marshalli Ramesha & Ramamurthy, 2011 (non Baris marshalli Hustache, 1938. New replacement names are: Baris ramamurthyi Alonso-Zarazaga nom. nov. for Baris bimaculata Pajni & Kohli, 1990 (non Hustache, 1932; Archarius (Archarius kwonleeanus Alonso-Zarazaga nom. nov. for Archarius (Archarius parvus (Kwon & Lee, 1990 (non Archarius (Archarius parvus (Hong & Wang, 1987, a fossil species; Curculio (Curculio zhangianus Alonso-Zarazaga nom. nov. for Curculio (Curculio helleri Pelsue & Zhang, 2002 (non Curculio (Curculio helleri (Voss, 1932; Lixus trichromus Alonso-Zarazaga nom. nov. for Lixus

  16. Pepper Weevil (Coleoptera: Curculionidae) Preferences for Specific Pepper Cultivars, Plant Parts, Fruit Colors, Fruit Sizes, and Timing.

    Science.gov (United States)

    Seal, Dakshina R; Martin, Cliff G

    2016-01-01

    Peppers (Capsicum spp.) are an important crop in the USA, with about 32,000 ha cultivated in 2007, which resulted in $588 million in farm revenue. The pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae), is the most troublesome insect pest of peppers in the southern United States. It is therefore urgent to find different vulnerabilities of pepper cultivars, fruit and plants parts, fruit colors and sizes, and timing to infestation by A. eugenii. Also relevant is testing whether fruit length and infestation state affect fruit numbers, weights, and proportions of fruit that are infested. Counts of A. eugenii adults and marks from oviposition and feeding suggested that C. chinense Jacquin "Habanero" was least susceptible, and C. annuum L. cultivars "SY" and "SR" were most susceptible. Comparison of plant parts and fruit sizes revealed that A. eugenii preferred the peduncle, calyx, and top of pepper fruits over the middle, bottom, leaves, or remainder of flowers. Anthonomus eugenii does not discriminate between green or yellow fruit color nor vary diurnally in numbers. Based on adult counts, medium to extra-large fruits (≥1.5 cm long) attracted more weevils than small fruits (<1.5 cm). However based on proportions of fruit numbers or fruit weights that were infested, there were no differences between large and small fruits. Choice of pepper cultivar can thus be an important part of an IPM cultural control program designed to combat A. eugenii by reduced susceptibility or by synchronous fruit drop of infested fruits. Our results are potentially helpful in developing scouting programs including paying particular attention to the preferred locations of adults and their sites of feeding and oviposition on the fruit. The results also suggested the potential value of spraying when the fruits are still immature to prevent and control infestation. PMID:26959066

  17. Taxonomic revision of Madagascan Rhantus (Coleoptera, Dytiscidae, Colymbetinae with an emphasis on Manjakatompo as a conservation priority

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    Anna Hjalmarsson

    2013-11-01

    Full Text Available We review the diving-beetle genus Rhantus Dejean of Madagascar (Coleoptera, Dytiscidae, Colymbetinae based on museum collection holdings and recently collected expedition material. Both morphology and DNA is used to test species boundaries, in particular whether newly collected material from the Tsaratanana mountains in the north represent a new species or are conspecific with Rhantus manjakatompo Pederzani and Rocchi 2009, described based on a single male specimen from the central Ankaratra mountains. DNA of the holotype of R. manjakatompo was successfully extracted in a non-destructive way and sequenced. The general mixed Yule coalescent model applied to an ultrametric tree constructed from mitochondrial cytochrome c oxidase subunit I (COI sequence data delimited three species. Morphological characters supported the same species unambiguously. We therefore recognise three species of Rhantus to occur in Madagascar: R. latus (Fairmaire, 1869, R. bouvieri Régimbart, 1900 and R. manjakatompo Pederzani and Rocchi, 2009. All three species are endemic to Madagascar and restricted to the highlands of the island. Rhantus stenonychus Régimbart, 1895, syn. n., is considered a junior synonym of R. latus. We designate lectotypes for R. bouvieri and R. goudoti Sharp, 1882, the latter a junior synonym of R. latus. We provide descriptions, a determination key, SEM-images of fine pronotal and elytral structures, distribution maps, habitus photos, and illustrations of male genitalia and pro- and mesotarsal claws. We discuss the role of the Manjakatompo forest as a refugium for Madagascan Rhantus diversity and other endemics of the montane central high plateau.

  18. Potential of two entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Coleoptera: Scarabaeidae), as biological control agents against the June beetle.

    Science.gov (United States)

    Erler, Fedai; Ates, A Ozgur

    2015-01-01

    The aim of this study was to evaluate the effectiveness of the entomopathogenic fungi (EPF), Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) strain PPRI 5339 [BroadBand, an emulsifiable spore concentrate (EC) formulation] and Metarhizium anisopliae (Metsch.) Sorokin (Hypocreales: Clavicipitaceae) strain F52 [Met52, both EC and granular (GR) formulations] against the larvae of Polyphylla fullo (L.) (Coleoptera: Scarabaeidae). Larvicidal bioassays were performed in foam boxes (100 by 75 by 50 cm; length by width by height), containing moist soil medium with some humus and potato tubers as food. Although the B. bassiana product (min. 4 × 10(9) conidia/ml) was applied at 100, 150, and 200 ml/100 l water; M. anisopliae strain F52 was applied at 500, 1,000, and 1,500 g/m(3) of moist soil medium for GR (9 × 10(8) cfu/g) and 75, 100, and 125 ml/100 l water for EC (5.5 × 10(9) conidia/ml) formulation. Both fungi were pathogenic to larvae of the pest; however, young larvae (1st and 2nd instars) were more susceptible to infection than older ones (3rd instar). Mortality rates of young and older larvae varied with conidial concentration of both fungi and elapsed time after application. The B. bassiana product was more effective than both of the formulations of the M. anisopliae product, causing mortalities up to 79.8 and 71.6% in young and older larvae, respectively. The highest mortality rates of young and older larvae caused by the M. anisopliae product were 74.1 and 67.6% for the GR formulation, 70.2 and 61.8% for the EC formulation, respectively. These results may suggest that both fungi have potential to be used for management of P. fullo. PMID:25881632

  19. Determinants of acute mortality of Hippodamia convergens (Coleoptera: Coccinellidae) to ultra-low volume permethrin used for mosquito management

    Science.gov (United States)

    Preftakes, Collin J.; Bodin, Jennifer L.; Brown, Christopher R.; Piccolomini, Alyssa M.; Schleier, Jerome J.

    2016-01-01

    There are relatively few experimental studies and risk assessments of the effects on non-target insects from ultra-low volume (ULV) insecticides used for management of adult mosquitoes. Therefore, we evaluated factors that may influence the ability of an insect to intercept the insecticide at the time of application by using Hippodamia convergens (Coleoptera: Coccinellidae) in field bioassay experiments in 2011 and 2015. Treatment factors included different distances, two cage heights (ground-level and 1.5 m above ground) to the point of the application, and covered vs. uncovered cage faces (2015 only). Insecticides used included a water-based formulation (Aqua-Reslin®) and an oil-based formulation (Permanone® 30-30) of permethrin. Cage height was highly significant both years, with much less acute (i.e., short-term exposure) mortality at ground-level compared with 1.5 m. In 2011, acute mortality was less at ground-level (mean = 3.2%, median = 0%) compared to 1.5 m (mean = 85.2%, median = 100%). Cage type also was highly significant, with less mortality in covered cages compared to uncovered cages. Mortality by cage height and cage type was as follows: ground level, covered cage (mean = 2.8%, median = 0.1%); ground level, uncovered cage (mean = 41.9%, median = 9.6%); 1.5 m, covered cage (mean = 6.8%, median = 0%); 1.5 m, uncovered cage (mean = 83.7%, median = 100%). Results suggest that acute mortality to non-target insects may vary considerably based on their height and their ability to directly intercept the insecticide as the aerosol passes through the area being sprayed. PMID:27366655

  20. Predaceous water beetles (Coleoptera, Hydradephaga) of the Lake St Lucia system, South Africa: biodiversity, community ecology and conservation implications.

    Science.gov (United States)

    Perissinotto, Renzo; Bird, Matthew S; Bilton, David T

    2016-01-01

    Water beetles are one of the dominant macroinvertebrate groups in inland waters and are excellent ecological indicators, reflecting both the diversity and composition of the wider aquatic community. The predaceous water beetles (Hydradephaga) make up around one-third of known aquatic Coleoptera and, as predators, are a key group in the functioning of many aquatic habitats. Despite being relatively well-known taxonomically, ecological studies of these insects in tropical and subtropical systems remain rare. A dedicated survey of the hydradephagan beetles of the Lake St Lucia wetlands (South Africa) was undertaken between 2013 and 2015, providing the first biodiversity census for this important aquatic group in the iSimangaliso Wetland Park, a UNESCO World Heritage Site within the Maputaland biodiversity hotspot. A total of 32 sites covering the entire spectrum of waterbody types were sampled over the course of three collecting trips. The Lake St Lucia wetlands support at least 68 species of Hydradephaga, a very high level of diversity comparing favourably with other hotspots on the African continent and elsewhere in the world and a number of taxa are reported for South Africa for the first time. This beetle assemblage is dominated by relatively widespread Afrotropical taxa, with few locally endemic species, supporting earlier observations that hotspots of species richness and centres of endemism are not always coincident. Although there was no significant difference in the number of species supported by the various waterbody types sampled, sites with the highest species richness were mostly temporary depression wetlands. This contrasts markedly with the distribution of other taxa in the same system, such as molluscs and dragonflies, which are most diverse in permanent waters. Our study is the first to highlight the importance of temporary depression wetlands and emphasises the need to maintain a variety of wetland habitats for aquatic conservation in this biodiverse

  1. Host Resistance of Five Fraxinus Species to Agrilus planipennis (Coleoptera: Buprestidae) and Effects of Paclobutrazol and Fertilization.

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    Tanis, Sara R; Mccullough, Deborah G

    2015-04-01

    Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) has killed millions of Fraxinus spp. trees in North America. While all Fraxinus species assessed to date can be colonized, A. planipennis attraction to host trees varies among species and with tree health. We established a plantation of 105 trees (21 trees each of four North American species Fraxinus americana L., Fraxinus nigra Marshall, Fraxinus pennsylvanica Marshall, Fraxinus quadrangulata Michaux, and the Asian species, Fraxinus mandshurica Ruprecht), and determined whether resistance to A. planipennis could be enhanced by fertilizer or paclobutrazol applications. Differences among species overshadowed most treatment effects. In 2010, A. planipennis survival over 14 d was 53% when beetles were caged with F. nigra, 30-32% when beetles were caged with F. americana, F. pennsylvanica, or F. mandshurica, and only 14% for beetles caged with F. quadrangulata. In 2011, beetle survival was lower for beetles caged with F. quadrangulata (33%) than F. americana (72%) or F. mandshurica (80%). In 2010 and 2011, leaf weight consumed by beetles was the same among Fraxinus species. However, beetles caged on F. quadrangulata consumed less leaf area than that by beetles caged with other ash species. In 2011, when trees were exposed to wild A. planipennis, larval density (per m(2)) was highest on F. nigra (235.9 ± 36.41) and F. pennsylvanica (220.1 ± 39.77), intermediate on F. americana (40.7 ± 11.61), and lowest on F. quadrangulata and F. mandshurica (2.0 ± 0.98 and 1.5 ± 0.67, respectively). Results indicate F. quadrangulata and F. mandshurica were relatively resistant to A. planipennis, F. nigra and F. pennsylvanica were highly vulnerable, and F. americana was intermediate. PMID:26313182

  2. All European Ash Species are Susceptible to Emerald Ash Borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae – a Far Eastern Invader

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    Yu. N. Baranchikov

    2014-12-01

    Full Text Available Emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae – an example of a destructive invasive insect which abruptly enlarged its initially East-Asian area in 80–90s of the previous century. Nowadays this species is the main pest of ash (Fraxinus trees in USA and Canada and is quickly spreading over 11 administrative regions of Russia. It is very important to determine a list of possible host plants of A. planipennis for the pest risk assessment of invasion of this pest over the territory of Central and Western Europe. In its native area – North-Eastern Asia – this buprestid is a secondary consumer of dying trees of East-Asian ash species F. сhinensis and F. mandshurica. Healthy trees of these species are highly resistant to the pest. No examples of resistant ash species were found at North American continent. Documentary data are presented for the first time on infestation of three European ash species at the territory of the Main Botanical Garden of the Russian Academy of Sciences in Moscow, Russia by the emerald ash borer. At the first time this pest was registered at the garden in 2011. During the period of 2010–2014 EAB killed from 70 to 100 % of trees of European ash species: Fraxinus excelsior, F. angustifolia (= F. oxycarpa and F. ornus. At the same period from 81 to 90 % of specimens of North American ash species (F. pennsylvanica and F. americana were killed by this buprestid. Simultaneously dead trees of Asian species F. mandshurica and F. chinensis (= F. rhynchophyla did not carry any trace of EAB infestation. This case study is a good example of the «sentinel trees» concept’s usefulness. Arboretums with collections of non-native plants may serve as «ecological traps» for the local pests and pathogens – potential invasive organisms in the source regions of introduced plants.

  3. Effects of host plant and larval density on intraspecific competition in larvae of the emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Duan, Jian J; Larson, Kristi; Watt, Tim; Gould, Juli; Lelito, Jonathan P

    2013-12-01

    Competition for food, mates, and space among different individuals of the same insect species can affect density-dependent regulation of insect abundance or population dynamics. The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a serious invasive pest of North American ash (Fraxinus spp.) trees, with its larvae feeding in serpentine galleries between the interface of sapwood and phloem tissues of ash trees. Using artificial infestation of freshly cut logs of green ash (Fraxinus pennsylvanica Marshall) and tropical ash (Fraxinus uhdei [Wenzig] Lingelsh) with a series of egg densities, we evaluated the mechanism and outcome of intraspecific competition in larvae of A. planipennis in relation to larval density and host plant species. Results from our study showed that as the egg densities on each log (1.5-6.5 cm in diameter and 22-25 cm in length) increased from 200 to 1,600 eggs per square meter of surface area, larval survivorship declined from ≍68 to 10% for the green ash logs, and 86 to 55% for tropical ash logs. Accordingly, larval mortality resulting from cannibalism, starvation, or both, significantly increased as egg density increased, and the biomass of surviving larvae significantly decreased on both ash species. When larval density was adjusted to the same level, however, larval mortality from intraspecific competition was significantly higher and mean biomasses of surviving larvae was significantly lower in green ash than in tropical ash. The role of intraspecific competition of A. planipennis larvae in density-dependent regulation of its natural population dynamics is discussed. PMID:24280666

  4. Predaceous water beetles (Coleoptera, Hydradephaga) of the Lake St Lucia system, South Africa: biodiversity, community ecology and conservation implications

    Science.gov (United States)

    Perissinotto, Renzo; Bird, Matthew S.; Bilton, David T.

    2016-01-01

    Abstract Water beetles are one of the dominant macroinvertebrate groups in inland waters and are excellent ecological indicators, reflecting both the diversity and composition of the wider aquatic community. The predaceous water beetles (Hydradephaga) make up around one-third of known aquatic Coleoptera and, as predators, are a key group in the functioning of many aquatic habitats. Despite being relatively well-known taxonomically, ecological studies of these insects in tropical and subtropical systems remain rare. A dedicated survey of the hydradephagan beetles of the Lake St Lucia wetlands (South Africa) was undertaken between 2013 and 2015, providing the first biodiversity census for this important aquatic group in the iSimangaliso Wetland Park, a UNESCO World Heritage Site within the Maputaland biodiversity hotspot. A total of 32 sites covering the entire spectrum of waterbody types were sampled over the course of three collecting trips. The Lake St Lucia wetlands support at least 68 species of Hydradephaga, a very high level of diversity comparing favourably with other hotspots on the African continent and elsewhere in the world and a number of taxa are reported for South Africa for the first time. This beetle assemblage is dominated by relatively widespread Afrotropical taxa, with few locally endemic species, supporting earlier observations that hotspots of species richness and centres of endemism are not always coincident. Although there was no significant difference in the number of species supported by the various waterbody types sampled, sites with the highest species richness were mostly temporary depression wetlands. This contrasts markedly with the distribution of other taxa in the same system, such as molluscs and dragonflies, which are most diverse in permanent waters. Our study is the first to highlight the importance of temporary depression wetlands and emphasises the need to maintain a variety of wetland habitats for aquatic conservation in this

  5. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations.

    Science.gov (United States)

    Beaudoin-Ollivier, L; Flori, A; Coffi, A; Cros, D; Glitho, I; Nodichao, L

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineensis × E. oleifera) × E. guineensis backcrosses. Females laid their eggs similarly on all breeding populations, with a preference for the E. guineensis La Mé origin. The average hatching rate reached 80% for the La Mé origin as opposed to 28% for the Deli origin. The mortality rates for the larval instars were greater on E. oleifera, on certain backcrosses and on the Deli origin of E. guineensis. Development at the second- and third- larval instars was the most affected, with a mortality rate of three to five times greater than that seen on La Mé. Epidermis and cuticle measurements indicated which breeding populations were suitable or unsuitable for the development of C. lameensis. E. guineensis, with its thin epidermis (12 µm) and cuticle (2 µm), proved to be highly susceptible to C. lameensis attacks. On the other hand, E. oleifera, which is very resistant, exhibited a thicker epidermis (17 µm) and cuticle (4 µm). The breeding populations were thus classified according to the positive or negative influence they exerted on the insect's egg laying and feeding. PMID:26113512

  6. Virulência de nematóides entomopatogênicos (Nematoda: Rhabditida a Sphenophorus levis (Coleoptera: Curculionidae

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    Fernando Henrique Carvalho Giometti

    2011-01-01

    Full Text Available O bicudo da cana-de-açúcar, Sphenophorus levis (Coleoptera: Curculionidae, é uma importante praga de solo dos canaviais no Estado de São Paulo, ocasionando prejuízos de até 30 t de cana/ha/ano. Visando ao controle biológico desta praga, esse estudo teve por objetivo avaliar a virulência de 17 isolados de nematóides entomopatogênicos do gênero Steinernema e Heterorhabditis contra adultos de S. levis, e o efeito dos quatro nematóides mais virulentos em três dosagens cada um. A pesquisa foi realizada em condições de laboratório, sendo realizados quatro ensaios para o estudo de virulência. Os nematóides selecionados como mais virulentos em cada teste foram S. brazilense IBCB n6, Heterorhabditis sp. IBCB n10, Heterorhabditis sp. IBCB n24 e Heterorhabditis sp. IBCB n44, sendo os únicos que diferenciaram significativamente das respectivas testemunhas com 45%, 40%, 20% e 31% de mortalidade do inseto na concentração de 240 juvenis infectivos (JI/inseto. No teste de dose, os isolados de Heterorhabditis identificados como IBCB n10, IBCB n24 e IBCB n44 destacaram-se quanto à virulência para os adultos do bicudo da cana-de-açúcar, diferindo significativamente da testemunha na maior dose avaliada (1200 JI/inseto com níveis de mortalidade do inseto de 60%, 65,7% e 74,3% respectivamente.

  7. Ácaros asociados al coleóptero Passalus cognatus (Coleoptera: Passalidae de Los Tuxtlas, Veracruz, México

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    Gabriel A Villegas-Guzmán

    2008-09-01

    Full Text Available Se revisaron 35 ejemplares de Passalus cognatus Truqui recolectados en Los Tuxtlas, Veracruz, México. En 20 de ellos se encontraron 245 ácaros pertenecientes a ocho especies, ocho géneros, ocho familias y tres subórdenes. Las especies de acáros más abundantes fueron Uroobovella californiana Wisniewski y Hirschmann (35%, Euzercon hyatti Hunter y Rosario (20% y Uropoda sp. (17.5%. Las zonas de fijación preferidas por los ácaros fueron las coxas; en menor proporción el mesoesternón y los húmeros. Los pasálidos infestados presentaron de 1 a 40 ácaros, con un promedio de 12. El número de especies de ácaros por pasálido infestado fue de 1 a 4.Mites associated to the Coleopteran Passalus cognatus (Coleoptera:Passalidae from Los Tuxtlas, Veracruz, Mexico. There are few records of mites associated with the tropical coleopterans of Mexico. We examined 35 passalid beetles (bessbugs Passalus cognatus from Los Tuxtlas region in Veracruz State, Mexico. Twenty of them had a total of 245 mites (representing eight species, eight genera, eight families and three suborders. The most abundant species were Uroobovella californiana Wisniewski & Hirschmann (35%, Euzercon hyatti Hunter & Rosario (20%, and Uropoda sp. (17.5%. The preferred attachment areas were the coxae; followed by the mesosternum and the humeri. Each beetle had 1 to 40 mites (average: 12; and we found 1-4 mite species per beetle. Rev. Biol. Trop. 56 (3: 1261-1268. Epub 2008 September 30.

  8. Coleoptera Scarabaeoidea de la región de Teziutlán, Puebla, México

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    Adrián MUÑOZ-HERNÁNDEZ

    2008-01-01

    Full Text Available Con el propósito de evaluar la riqueza, abundancia y fenología de las especies de Coleoptera Scarabaeoidea o Lamellicornia de la región de Teziutlán, Puebla, se realizaron colectas mensuales entre mayo de 1999 y junio de 2000, en sitios con bosques de pino-encino-liquidámbar o zonas de cultivo situados entre 1,990 y 2,000 m de altitud. Se capturaron 2,082 ejemplares que representan a 59 especies de 29 géneros, 18 tribus y 7 subfamilias de Melolonthidae, Scarabaeidae y Passalidae, entre las que destacan Phyllophaga godmani (Bates, Isonychus piperitus Bates, Anomala terroni Morón, Chrysina peruviana Kirby, Plusiotis aurofoveata Morón, Cyclocephala fasciolata Bates, Dynastes hyllus Chevrolat, Deltochilum mexicanum Bates, Ontherus mexicanus Harold, Onthophagus cyanellus Bates, Passalus punctatostriatus Percheron, Proculejus hirtus (Truqui y Odontotaenius zodiacus Truqui. La riqueza específica es elevada y representativa de ese tipo de bosques a pesar de la perturbación existente. Las especies más abundantes fueron Phyllophaga vetula (Horn, P. xanthe (Bates, Macrodactylus mexicanus Burmeister, Hoplia squamifera Burmeister, Cyclocephala jalapensis Casey, C. weidneri Endrodi, y Chrysina macropus Francillon. Los adultos de la mayor parte de las especies están activos entre abril y junio. De acuerdo con el índice de Simpson la fauna de lamelicornios de Teziutlán presenta un 80% de similitud con la fauna de las montañas del noreste de Hidalgo, un 33% con la región vecina de Cuetzalan, Puebla, y un 17% con el área de Los Tuxtlas, Veracruz. Se incluye una clave para separar las 59 especies encontradas.

  9. Coleoptera scarabaeoidea de la región de Teziutlán, Puebla, México

    Directory of Open Access Journals (Sweden)

    Adri\\u00E1n Mu\\u00F1oz Hern\\u00E1ndez

    2008-01-01

    Full Text Available Con el propósito de evaluar la riqueza, abundancia y fenología de las especies de Coleoptera Scarabaeoidea o Lamellicornia de la región de Teziutlán, Puebla, se realizaron colectas mensuales entre mayo de 1999 y junio de 2000, en sitios con bosques de pino-encino-liquidámbar o zonas de cultivo situados entre 1,990 y 2,000 m de altitud. Se capturaron 2,082 ejemplares que representan a 59 especies de 29 géneros, 18 tribus y 7 subfamilias de Melolonthidae, Scarabaeidae y Passalidae, entre las que destacan Phyllophaga godmani (Bates, Isonychus piperitus Bates, Anomala terroni Morón, Chrysina peruviana Kirby, Plusiotis aurofoveata Morón, Cyclocephala fasciolata Bates, Dynastes hyllus Chevrolat, Deltochilum mexicanum Bates, Ontherus mexicanus Harold, Onthophagus cyanellus Bates, Passalus punctatostriatus Percheron, Proculejus hirtus (Truqui y Odontotaenius zodiacus Truqui. La riqueza específica es elevada y representativa de ese tipo de bosques a pesar de la perturbación existente. Las especies más abundantes fueron Phyllophaga vetula (Horn, P. xanthe (Bates, Macrodactylus mexicanus Burmeister, Hoplia squamifera Burmeister, Cyclocephala jalapensis Casey, C. weidneri Endrodi, y Chrysina macropus Francillon. Los adultos de la mayor parte de las especies están activos entre abril y junio. De acuerdo con el índice de Simpson la fauna de lamelicornios de Teziutlán presenta un 80% de similitud con la fauna de las montañas del noreste de Hidalgo, un 33% con la región vecina de Cuetzalan, Puebla, y un 17% con el área de Los Tuxtlas, Veracruz. Se incluye una clave para separar las 59 especies encontradas.

  10. Biological aspects of Leucothyreus alvarengai Frey and Leucothyreus aff. semipruinosus Ohaus (Coleoptera, Melolonthidae, Rutelinae in crop succession at central Brazil

    Directory of Open Access Journals (Sweden)

    Alex Furquim Pereira

    2013-09-01

    Full Text Available Biological aspects of Leucothyreus alvarengai Frey and Leucothyreus aff. semipruinosus Ohaus (Coleoptera, Melolonthidae, Rutelinae in crop succession at central Brazil. Beetles of the family Melolonthidae make up a large group and some species are considered pests of planted crops. Little information is available on the basic biological aspects of the genus Leucothyreus, such as association with cultivated crops and their occurrence periods. Therefore studies were developed in soybean and corn crops in Tangará da Serra, Mato Grosso, Brazil, with the objective of studying the occurrence and biological aspects of Leucothyreus alvarengai Frey and Leucothyreus aff. semipruinosus Ohaus. For acquisition of immature specimens of both species, in April 2011 sampling was performed in corn fields, in July and October in the fallow area, and in soybeans fields planted in December; in 2012 sampling was performed in January and February in soybean fields and in March in corn fields. In 2011 the total number of larvae obtained in April, July, October and December were 100, 6, 30 and 27, and in January, February and March of 2012 these quantities were 32, 52 and 65 larvae, respectively. In all sampling events the larvae of L. alvarengai were collected in greater quantity. At the beginning of the reproductive period of L. alvarengai and L. aff. semipruinosus, it was observed that the adults began to fly and soon after started oviposition in the field in September. The appearance of larvae coincides with the time of soybean planting in the field, thus the larvae feed on roots of soybean plants at the beginning of their development and the cycle from egg to adult of the two species was completed in one year.

  11. The response of ground beetles (Coleoptera: Carabidae) to selection cutting in a South Carolina bottomland hardwood forest.

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael, D.; Hanula, James L.; Horn, Scott; Kilgo, John, C.; Moorman, Christopher, E.

    2005-04-01

    We compared the response of ground beetles (Coleoptera: Carabidae) to the creation of canopy gaps of different size (0.13, 0.26, and 0.50 ha) and age (1 and 7 years) in a bottomland hardwood forest (South Carolina, USA). Samples were collected four times in 2001 by malaise and pitfall traps placed at the center and edge of each gap, and 50 m into the surrounding forest. Species richness was higher at the center of young gaps than in old gaps or in the forest, but there was no statistical difference in species richness between old gaps and the forests surrounding them. Carabid abundance followed the same trend, but only with the exclusion of Semiardistomis viridis (Say), a very abundant species that differed in its response to gap age compared to most other species. The carabid assemblage at the gap edge was very similar to that of the forest, and there appeared to be no distinct edge community. Species known to occur in open or disturbed habitats were more abundant at the center of young gaps than at any other location. Generalist species were relatively unaffected by the disturbance, but one species (Dicaelus dilatatus Say) was significantly less abundant at the centers of young gaps. Forest inhabiting species were less abundant at the centers of old gaps than in the forest, but not in the centers of young gaps. Comparison of community similarity at various trapping locations showed that communities at the centers of old and young gaps had the lowest similarity (46.5%). The community similarity between young gap centers and nearby forest (49.1%) and old gap centers and nearby forest (50.0%) was similarly low. These results show that while the abundance and richness of carabids in old gaps was similar to that of the surrounding forest, the species composition between the two sites differed greatly.

  12. Effect of temperature and tree species on damage progression caused by whitespotted sawyer (Coleoptera: Cerambycidae) larvae in recently burned logs.

    Science.gov (United States)

    Bélanger, Sébastien; Bauce, Eric; Berthiaume, Richard; Long, Bernard; Labrie, Jacques; Daigle, Louis-Frédéric; Hébert, Christian

    2013-06-01

    The whitespotted sawyer, Monochamus scutellatus scutellatus (Say) (Coleoptera: Ce-rambycidae), is one of the most damaging wood-boring insects in recently burned boreal forests of North America. In Canada, salvage logging after wildfire contributes to maintaining the timber volume required by the forest industry, but larvae of this insect cause significant damage that reduces the economic value of lumber products. This study aimed to estimate damage progression as a function of temperature in recently burned black spruce (Picea mariana (Miller) Britton, Sterns, and Poggenburg) and jack pine (Pinus banksiana Lambert) trees. Using axial tomographic technology, we modeled subcortical development and gallery depth progression rates as functions of temperature for both tree species. Generally, these rates were slightly faster in black spruce than in jack pine logs. Eggs laid on logs kept at 12 degrees C did not hatch or larvae were unable to establish themselves under the bark because no larval development was observed. At 16 degrees C, larvae stayed under the bark for > 200 d before penetrating into the sapwood. At 20 degrees C, half of the larvae entered the sapwood after 30-50 d, but gallery depth progression stopped for approximately 70 d, suggesting that larvae went into diapause. The other half of the larvae entered the sapwood only after 100-200 d. At 24 and 28 degrees C, larvae entered the sapwood after 26-27 and 21 d, respectively. At 28 degrees C, gallery depth progressed at a rate of 1.44 mm/d. Temperature threshold for subcortical development was slightly lower in black spruce (12.9 degrees C) than in jack pine (14.6 degrees C) and it was 1 degrees C warmer for gallery depth progression for both tree species. These results indicate that significant damage may occur within a few months after fire during warm summers, particularly in black spruce, which highlights the importance of beginning postfire salvage logging as soon as possible to reduce economic

  13. Field trial of diatomaceous earth in cotton gin trash against the larger black flour beetle, Cynaeus angustus (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    McIntyre, N E; Porter, P

    2004-04-01

    The larger black flour beetle, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), is an agricultural and home nuisance pest in North America. In the Southern High Plains of Texas, the larger black flour beetle is associated with cotton gin trash, by-products of cotton ginning that are field stored in large piles for economic reasons. Larger black flour beetle overwinter in gin trash piles but may disperse by the millions in summer and autumn, entering houses as far as 2 km away where they cause distress to homeowners. Because > 1.2 billion kg of gin trash is produced annually in Texas alone, the potential consequences of the larger black flour beetle are enormous. We conducted a field experiment that evaluated the efficacy of diatomaceous earth (DE) on the abundance of the larger black flour beetle in gin trash. There were no significant differences in numbers of larger black flour beetle among treatments and controls (mean number of adults summed over time: controls = 115.41, layered treatment = 87.60, top and bottom treatment = 96.50, bottom treatment = 115.16). There were sufficient numbers of beetles in treated piles to still pose a potential home nuisance problem, likely because the moisture content of field-stored gin trash is too high for DE to work effectively. Therefore, treating cotton gin trash with diatomaceous earth will probably be unable to prevent home infestations of larger black flour beetle. Location within a gin trash pile and season influenced pest numbers, which has implications for long-term field storage of cotton gin trash. PMID:15154486

  14. The Ceratocanthinae of Ulu Gombak: high species richness at a single site, with descriptions of three new species and an annotated checklist of the Ceratocanthinae of Western Malaysia and Singapore (Coleoptera, Scarabaeoidea, Hybosoridae

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    Alberto Ballerio

    2010-01-01

    Full Text Available The remarkable species richness of Ceratocanthinae (Coleoptera: Scarabaeoidea: Hybosoridae found at Ulu Gombak (Selangor, West Malaysia, a secondary rainforest research station, is discussed. Eighteen species have been collected, mainly in nests of termites (Isoptera and bess beetles (Coleoptera: Passalidae. Among them at least seven are new species, three of them here described: Madrasostes hashimi sp. n., Madrasostes mirificum sp. n., and Pterorthochaetes tsurui sp. n. Four other species (Madrasostes agostii Paulian, Madrasostes clypeale Paulian, Madrasostes depressum Paulian, and Madrasostes simplex Paulian are recorded for the first time for West Malaysia and three for new states within West Malaysia (Pterorthochaetes insularis Gestro, Madrasostes malayanum Paulian and Madrasostes sculpturatum Paulian. A checklist of the 34 Ceratocanthinae recorded so far from West Malaysia and Singapore is provided with taxonomic, distributional and morphological remarks on some species.

  15. Los Escarabeidos y Geotrúpidos de la Comunidad de Madrid: lista de especies, distribución geográfica y patrones de diversidad (Coleoptera, Scarabaeoidea, Scarabaeidae y Geotrupidae

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    Lobo, J. M.

    2006-05-01

    Full Text Available In this work we summarize the main characteristics of Geotrupidae and Scarabaeidae (Coleoptera, Scarabaeoidea fauna inhabiting Comunidad de Madrid, including an updated checklist of species as well as maps representing the observed and potential distributions of all species. Geographical variation in species richness, rarity or endemicity was also estimated, with a brief discussion on the geographical pattern obtained.

    En este trabajo se resumen las características fundamentales de la fauna de Geotrupidae y Scarabaeidae (Coleoptera, Scarabaeoidea que habita la Comunidad de Madrid, presentándose un inventario actualizado así como mapas que representan la distribución observada y potencial de todas las especies. Finalmente, se estima la variación geográfica de la riqueza de especies, la rareza o la endemicidad, discutiendo brevemente el patrón geográfico encontrado.

  16. Flutuação Populacional de Rhynchophorus palmarum L. (Coleoptera: Curculionidae em Plantios de Palma de Óleo em Roraima

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    2015-08-01

    Full Text Available Esta pesquisa teve como objetivo avaliar a flutuação populacional de Rhynchophorus palmarum L. (Coleoptera: Curculionidae em plantios de Elaeis guineensis Jacq, em ecossistemas de savana e floresta no estado de Roraima. A pesquisa foi desenvolvida durante o período de janeiro de 2011 a fevereiro de 2012 nos campos experimentais da Embrapa Roraima: Monte Cristo e Caroebe, numa área de 2 hectares com a cultura da Palma de Óleo, em cada campo. Foram distribuídas armadilhas iscadas com roletes de cana-de-açúcar e feromônio de agregação para captura dos insetos no entorno dos plantios. Ocorreu flutuação de R. palmarum com picos populacionais nos meses de julho e agosto no ecossistema de savana e de abril a setembro no ambiente de floresta. A população de R. palmarum foi menor em meses de baixa precipitação pluviométrica nos dois ambientes avaliados com a Palma de Óleo em Roraima Population Fluctuation of Rhynchophorus palmarum L. (Coleoptera: Curculionidae in Plantations of Oil Palm in Roraima Abstract. This research aimed to evaluate the population fluctuation Rhynchophorus palmarum L. (Coleoptera: Curculionidae in Elaeis guineensis Jacq plantations in forest and savanna ecosystems in the state of Roraima. The research was conducted during the period from January 2011 to February 2012 in the experimental field of Embrapa Roraima: Monte Cristo and Caroebe in an area of 2 hectares with culture Oil Palm in each field. Traps baited with rollers cane sugar and aggregation pheromone for capturing insects in the vicinity of the plantations were distributed. Fluctuation occurred R. palmarum with population peaks in the months of July and August in the savanna ecosystem and from April to September in the forest environment. The population of R. palmarum was lower in months of low rainfall in the two environments assessed with a palm-oil-in Roraima.

  17. South American Coccinellidae (Coleoptera) : part 7, new name for Cyra Mulsant, review of Brachiacanthini genera, and systematic revision of Cleothera Mulsant, Hinda Mulsant and Serratitibia Gordon and Canepari, new genus

    OpenAIRE

    Gordon, Robert D.; Canepari, Claudio; Hanley, Guy A.

    2013-01-01

    Genera of Brachiacanthini (Coleoptera: Coccinellidae: Hyperaspidinae) are discussed and a key to all recognized genera provided. Cyrea, new genus, is proposed, and Serratitibia, new genus, is erected and revised, Cleothera Mulsant and Hinda Mulsant are recognized as valid genera and revised. Helesius caseyi Sicard is transferred to Hinda and recognized as a synonym of Hinda designata Mulsant, new synonymy. Brachiacantha brethesi (Korschefsky), Cleothera abendrothi Kirsch, Cleothera ambigua Mu...

  18. Natural history, ecology, and conservation of the genus Polyphylla Harris, 1841. 1. New species from the southwestern United States and Baja California, Mexico, with notes on distribution and synonymy (Coleoptera: Scarabaeidae: Melolonthinae)

    OpenAIRE

    La Rue, Delbert A.

    2016-01-01

    Four new species of the genus Polyphylla Harris (Coleoptera: Scarabaeidae: Melolonthinae) from the southwestern United States and Baja California, Mexico, are described and illustrated: Polyphylla anivallis, P. koso, P. morroensis, and P. socorriana. Two nomenclatural changes are proposed: Polyphylla ratcliffei Young is placed into synonymy with P. avittata Hardy and Andrews, new synonymy; P. uteana Tanner is removed from synonymy and reinstated as a valid species, reinstated status. The fema...

  19. Biology and Host-Range Testing of Laricobius kangdingensis sp. n. (Coleoptera: Derodontidae), a Newly Discovered Predator of Hemlock Woolly Adelgid, Adelges tsugae Annand (Homoptera: Adelgidae)

    OpenAIRE

    Gatton, Holly A.

    2005-01-01

    The biology and host-specificity of Laricobius kangdingensis sp. n. (Coleoptera: Derodontidae), a new predator of hemlock woolly adelgid (HWA), Adelges tsugae Annand (Homoptera: Adelgidae) were investigated and compared with that of a related predator of HWA, L. nigrinus Fender. Adults became active in June 2003; the males emerged first and in greater numbers than the females. The sex ratio of female to male was 1: 1.1. Laricobius kangdingensis sp. n. has four larval instars. The total nu...

  20. Una nueva especie de Meloe Linnaeus, 1758 del suroeste de Marruecos incluida en el grupo de M. (Eurymeloe) rugosus Marsham, 1802 (Coleoptera: Meloidae)

    OpenAIRE

    JOSÉ L. RUIZ; García-París, M.

    2015-01-01

    The scientific collections of the Museo Nacional de Ciencias Naturales (MNCN-CSIC, Madrid) hold an extense set of entomological materials collected in Morocco along the first decades of the XXth century by the preeminent naturalist M. Martínez de la Escalera. Morphological studies of the specimens of the genus Meloe Linnaeus, 1758 (Coleoptera: Meloidae) reveals the existence of populations morphologically differentiated along the coastal regions of Essaouira and Ifni. These populations are in...

  1. Bioactivity of Powder and Extracts from Garlic, Allium sativum L. (Alliaceae) and Spring Onion, Allium fistulosum L. (Alliaceae) against Callosobruchus maculatus F. (Coleoptera: Bruchidae) on Cowpea, Vigna unguiculata (L.) Walp (Leguminosae) Seeds

    OpenAIRE

    Abiodun A. Denloye

    2010-01-01

    Laboratory bioassays were conducted to investigate the bioactivity of powders, extracts, and essential oils from Allium sativum L. (Alliaceae) and A. fistulosum L. (Liliaceae) against adults, eggs, and larvae of Callosobruchus maculatus F. (Coleoptera: Bruchidae). On the basis of 48 hr median lethal toxicity (LC50), test plant powders and extracts from A. sativum were more toxic to C. maculatus adults than those from A. fistulosum. The 48 hr LC50 values for the powder against the test insect ...

  2. Eucnemid larvae of the Nearctic region. Part V: Fifth instar larval descriptions for eight species of Microrhagus Dejean, 1833 (Coleoptera: Eucnemidae: Melasinae: Dirhagini), with descriptions of four new species and notes on their biology

    OpenAIRE

    Otto, Robert L.

    2016-01-01

    Fifth instars for eight of the nine species of Microrhagus Dejean (Coleoptera: Eucnemidae: Melasinae: Dirhagini), all endemic in the Nearctic region, are described and illustrated. Biological information is provided, along with a brief discussion of the larval morphology among the eight Nearctic species of Microrhagus. Adult descriptions are provided for four new species. These new species include Microrhagus breviangularis new species, Microrhagus carinicollis new species, Microrhagus ...

  3. Fauna de Coleoptera no Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brasil: abundância e riqueza das famílias capturadas através de armadilhas de solo

    OpenAIRE

    Marinoni Renato C.; Ganho Norma G.

    2003-01-01

    This paper is a part of the studies on the Coleoptera fauna from Vila Velha, Ponta Grossa, Parana gathered through malaise and pitfall traps in sites with different floristic conditions. The present study deal with the data of pitfall trap captures, installed close to the malaise traps. The data were obtained weekly (52 samples), from September 1999 to August 2000. This survey was carried out on five areas, three of them with different plant succession stages (initial, intermediate, and advan...

  4. Dispersal of individuals of the flightless grassland ground beetle, Carabus hungaricus (Coleoptera: Carabidae), in three populations and what they tell us about mobility estimates based on mark-recapture

    Czech Academy of Sciences Publication Activity Database

    Elek, Z.; Drag, Lukáš; Pokluda, Pavel; Čížek, Lukáš; Bérces, S.

    2014-01-01

    Roč. 111, č. 5 (2014), s. 663-668. ISSN 1210-5759 Grant ostatní: Hungarian Academy of Sciences(HU) BO/00045/11/8; European Social Fund(CZ) CZ.1.07/2.3.00/20.0064 Institutional support: RVO:60077344 Keywords : Coleoptera * Carabidae * Carabus hungaricus Subject RIV: EH - Ecology, Behaviour Impact factor: 0.975, year: 2014 http://www.eje.cz/pdfs/eje/2014/05/10.pdf

  5. Litteratura Coleopterologica (1758–1900): a guide to selected books related to the taxonomy of Coleoptera with publication dates and notes

    Science.gov (United States)

    Bousquet, Yves

    2016-01-01

    Abstract Bibliographic references to works pertaining to the taxonomy of Coleoptera published between 1758 and 1900 in the non-periodical literature are listed. Each reference includes the full name of the author, the year or range of years of the publication, the title in full, the publisher and place of publication, the pagination with the number of plates, and the size of the work. This information is followed by the date of publication found in the work itself, the dates found from external sources, and the libraries consulted for the work. Overall, more than 990 works published by 622 primary authors are listed. For each of these authors, a biographic notice (if information was available) is given along with the references consulted. PMID:27231436

  6. A novel 8.7 kDa protease inhibitor from chan seeds (Hyptis suaveolens L.) inhibits proteases from the larger grain borer Prostephanus truncatus (Coleoptera: Bostrichidae).

    Science.gov (United States)

    Aguirre, Cesar; Valdés-Rodríguez, Silvia; Mendoza-Hernández, Guillermo; Rojo-Domínguez, Arturo; Blanco-Labra, Alejandro

    2004-05-01

    A novel trypsin inhibitor purified from chan seeds (Hyptis suaveolens, Lamiaceae) was purified and characterized. Its apparent molecular mass was 8700 Da with an isoelectric point of 3.4. Its N-terminal sequence showed a high content of acidic amino acids (seven out of 18 residues). Its inhibitory activity was potent toward all trypsin-like proteases extracted from the gut of the insect Prostephanus truncatus (Coleoptera: Bostrichidae), a very important pest of maize. This activity was highly specific, because among proteases from seven different insects, only those from P. truncatus and Manduca sexta (Lepidoptera: Sphingidae) were inhibited. This inhibitor has potential to enhance the defense mechanism of maize against the attack of P. truncatus. PMID:15142539

  7. Effet structurant de la plante hôte chez la bruche de l'arachide, Caryedon serratus (Olivier, 1790 (Coleoptera : Bruchidae

    Directory of Open Access Journals (Sweden)

    Sembène, M.

    2012-01-01

    Full Text Available Structuring effect of the host plant in the groundnut bruchid, Caryedon serratus (Olivier, 1790 (Coleoptera: Bruchidae. Twenty-six samples of the groundnut seed-beetle which were reared from pods of five different host plants (Arachis hypogaea L., Bauhinia rufescens Lam., Cassia sieberiana DC., Piliostigma reticulatum (DC. Hochst. and Tamarindus indica L. in four localities of Senegal were compared using electrophoresis based on six loci of four enzymatic systems. The population structure of Caryedon serratus Olivier was analysed using Weir and Cockerham's estimator of Wright's F-statistics. θ value (0.235 and the dendrogram of Rogers'genetic distances revealed a high degree of genetic differentiation between host plants. Genetic analysis without C. sieberiana samples indicated that populations form host races which are partially isolated according to their host plants (θ = 0.035. Geographical distances between localities are not decisive for genetic structuration of C. serratus populations from a given host plant.

  8. Flea beetles (Coleoptera: Chrysomelidae: Alticinae collected by malaise trap method in Gölcük Natural Park (Isparta, Turkey, with a new record for Turkish fauna

    Directory of Open Access Journals (Sweden)

    Aslan Gül E.

    2012-01-01

    Full Text Available This study is based on Alticinae (Coleoptera: Chrysomelidae material collected by Malaise trapping which is different from other standardized collecting methods. A total of 19 flea beetle species belonging to 6 genera were collected from Gölcük Natural Park, Isparta (Turkey during 2009. The species are listed in a table together with distributional data in Turkey. Among them, Longitarsus curtus (Allard, 1860 is recorded for the first time in Turkey. L. monticola Kutschera, 1863 and L. curtus are recently separated synonyms and thus all data referring to the distribution of both species are currently important. Hence, the zoogeographical distribution of the new record is reviewed with some remarks; habitus and genitalia are illustrated.

  9. Elemental distribution in reproductive and neural organs of the Epilachna nylanderi (Coleoptera: Coccinellidae), a phytophage of nickel hyperaccumulator Berkheya coddii (Asterales: Asteraceae) by micro-PIXE.

    Science.gov (United States)

    Mesjasz-Przybyłowicz, Jolanta; Orłowska, Elżbieta; Augustyniak, Maria; Nakonieczny, Mirosław; Tarnawska, Monika; Przybyłowicz, Wojciech; Migula, Paweł

    2014-01-01

    The phenomenon of metal hyperaccumulation by plants is often explained by a pathogen or herbivore defense hypothesis. However, some insects feeding on metal hyperaccumulating plants are adapted to the high level of metals in plant tissues. Former studies on species that feed on the leaves of Berkheya coddii Roessler 1958 (Asteraceae), a nickel-hyperaccumulating plant, demonstrated several protective mechanisms involved in internal distribution, immobilization, and elimination of Ni from the midgut and Malpighian tubules. These species are mainly coleopterans, including the lady beetle, Epilachna nylanderi (Mulsant 1850) (Coleoptera: Coccinellidae), collected from the ultramafic ecosystem near Barberton in South Africa. By performing particle-induced X-ray emission microanalysis elemental microanalysis (PIXE), this study examined whether Ni may be harmful to internal body systems that decide on insect reactivity (central nervous system [CNS]), their reproduction, and the relationships between Ni and other micronutrients. Data on elemental distribution of nine selected elements in target organs of E. nylanderi were compared with the existing data for other insect species adapted to the excess of metals. Micro-PIXE maps of seven regions of the CNS showed Ni mainly in the neural connectives, while cerebral ganglia were better protected. Concentrations of other bivalent metals were lower than those of Ni. Testis, compared with other reproductive organs, showed low amounts of Ni. Zn was effectively regulated at physiological dietary levels. In insects exposed to excess dietary Zn, it was also accumulated in the reproductive organs. Comparison of E. nylanderii with other insects that ingest hyperaccumulating plants, especially chrysomelid Chrysolina clathrata (Clark) (Coleoptera: Chrysomelidae), showed lower protection of the CNS and reproductive organs. PMID:25399425

  10. 杨梅凋萎病菌异色拟盘多毛孢菌(Pestalotiopsis versicolor)的生物学特性研究%Biological characteristics of Pestalotiopsis versicolor,the agent of bayberry twig blight in Southern China

    Institute of Scientific and Technical Information of China (English)

    任海英; 李岗; 戚行江; 韦继光; 梁森苗; 郑锡良; 颜丽菊; 朱潇婷

    2013-01-01

    为了明确杨梅凋萎病的病原菌之一异色拟盘多毛孢菌( Pestalotiopsis versicolor)的生物学特性,对其3个代表菌株XJ27、XJ42、RA2-1在不同培养基、碳源、氮源、温度、pH、光照条件下的生长以及产孢情况进行研究。研究结果表明,在7种培养基上25℃培养时,PDA和PSA最适合菌株气生菌丝生长,菌丝生长速度快而茂盛。杨梅汁(BFA)上菌丝生长较快,但是不茂盛。燕麦培养基(OMA)和查氏培养基(CDA)中等适合,杨梅叶煎汁( BLA)和杨梅枝干煎汁( BBA)不适合气生菌丝生长。10种不同碳源培养基以及硝酸钾、硝酸铵、尿素和8种测定的氨基酸作氮源的培养基上3个菌株都生长良好,但是木糖培养基上生长较差。菌株在25~28℃能很好地生长,20℃和30℃生长中等,但是在5℃和35℃不能生长。 pH 5~10菌丝生长较好,但是pH 11.0菌丝不能很好生长。光照条件下,XJ27和XJ42生长后10天,有光照的产孢量大大多于没有光照的,但是RA2-1产孢不受光照影响。总之,杨梅凋萎病菌异色拟盘多毛孢菌具有广泛的培养基、碳源、氮源、温度以及pH的适应性,光照对部分菌株产孢性能有促进作用。%To study the biological characters of Pestalotiopsis versicolor, one of the agents of bayberry twig blight dis-ease, the effects of media type, carbon (C) and nitrogen (N) sources, temperature, pH, and light on P.versicolor were examined to determine optimal conditions for mycelial growth and sporulation .Three independent isolates showed a similar trend of growth and sporulation with different C and N sources .However , the growth rates varied significantly among seven agar media .Fungal growth rates were the highest on potato dextrose agar ( PDA) and pota-to sucrose agar (PSA).The fungi grew fast on bayberry fruit extract agar (BFA) but sparsely.Growth rates were moderate on oatmeal agar ( OMA) and

  11. Research into the Application of the Sterile-Male Release Technique to the Rhinoceros Beetle (Coleoptera:Scarabaeidae)

    International Nuclear Information System (INIS)

    The control of the Rhinoceros beetle, which causes considerable damage in palm and coconut plantations in the tropical regions of the world, meets with difficulties resulting from the habits of the insects. Chemical and biological control methods have not yet produced economically worthwhile results. It was therefore proposed, under the Joint Project established by the UN Special Fund and the South Pacific Commission for devising a method of controlling the main pest in this group, Oryctes rhinoceros, that the possibility be investigated of using against these coleoptera the technique of releasing males sterilized by radiation. Studies have been made involving the two most important species O. monoceros Ol. and O. rhinoceros L. The first stage was the development of a continuous breeding method, which had not hitherto been possible because of the large-scale slaughter arising from fungus attacks by Metarrhizium anisopliae Metsch. For the larvae the technique used involved first individual rearing, followed by group rearing tests with 100 or so insects in tanks. The average temperature in both cases was close to 30°C and the insects were fed on a mixture of rotten wood and leaf mould mixed with the faeces of domestic animals. The adults, fed on banana slices, are placed in troughs or tanks containing sieved-earth. It was thus possible to rear Oryctes in several successive cycles, avoiding the epizooic stage of Metarrhizium, with a multiplication rate from one generation to the next of close on 10, Tests on radiation sterilization procedures taking as variables the gamma-ray dose, the stage of the insect and the physiological state of the imago, showed that O. monoceros adults were sterilized by a single application of 4000 rad, with a slight reduction in their length of life. The same was true of O. rhinoceros, which however required a dose greater than 6000 rad to sterilize it. The mating urge and sexual vigour of the irradiated insects are, however, reduced

  12. Simulação do dano causado por larvas de Oryzophagus oryzae (Coleoptera: Curculionidae a cultivares de arroz irrigado

    Directory of Open Access Journals (Sweden)

    Martins José Francisco da Silva

    2004-01-01

    Full Text Available Oryzophagus oryzae (Costa Lima, 1936 (Coleoptera: Curculionidae é a praga-chave da cultura do arroz irrigado na Região Sul do Brasil. As larvas causam os principais danos ao cortarem drasticamente as raízes das plantas. Dois experimentos sobre simulação do dano larval foram realizados testando um equipamento cujo componente principal é uma lâmina metálica em forma de U, para corte das raízes. Raízes das cultivares BR-Irga 414 e Bluebelle, de ciclo biológico curto, e suscetíveis ao inseto e BR-Irga 410 e Dawn, de ciclo médio e resistentes, foram submetidas aos tratamentos de (1 corte artificial, com o simulador, (2 corte natural, pelas larvas e (3 sem corte, artificial ou natural, protegidas com inseticida. A simulação foi praticada na fase vegetativa das plantas, 31 dias pós-irrigação por inundação, época do pico da população larval nas raízes. Avaliaram-se dados sobre comprimento, peso de matéria seca e volume de raízes, imediatamente após a aplicação do tratamento de simulação e na pré-emissão de panículas, população larval em plantas submetidas ao dano natural, e produtividade de grãos. Na avaliação efetuada imediatamente após a simulação, em todas as cultivares, não ocorreu diferença entre índices de dano às raízes resultantes do uso do simulador e da alimentação de larvas. Na fase de pré-emissão das panículas, os índices de recuperação do sistema radicular das plantas submetidas aos danos natural e artificial, também foram similares. As cultivares de ciclo médio apresentaram maior recuperação dos tecidos radiculares e menor perda de produtividade de grãos, confirmando o maior grau de resistência a O. oryzae. Concluiu-se que o método de simulação é aplicável em pesquisas sobre resistência de arroz ao inseto, principalmente na identificação de genótipos tolerantes.

  13. Prediction of the key binding site of odorant-binding protein of Holotrichia oblita Faldermann (Coleoptera: Scarabaeida).

    Science.gov (United States)

    Zhuang, X; Wang, Q; Wang, B; Zhong, T; Cao, Y; Li, K; Yin, J

    2014-06-01

    The scarab beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae) is a predominant underground pest in the northern parts of China, and its larvae (grubs) cause great economic losses because of its wide range of host plants and covert habitats. Environmentally friendly strategies for controlling adults would have novel and broad potential applications. One potential pest management measure is the regulation of olfactory chemoreception to control target insect pests. In the process of olfactory recognition, odorant-binding proteins (OBPs) are believed to carry hydrophobic odorants from the environment to the surface of olfactory receptor neurons. To obtain a better understanding of the relationship between OBP structures and their ligands, homology modelling and molecular docking have been conducted on the interaction between HoblOBP1 and hexyl benzoate in the present study. Based on the results, site-directed mutagenesis and binding experiments were combined to describe the binding sites of HoblOBP1 and to explore its ligand-binding mechanism. After homology modelling of HoblOBP1, it was found that the three-dimensional structure of HoblOBP1 consists of six α-helices and three disulphide bridges that connect the helices, and the hydrophobic pockets are both composed of five helices. Based on the docking study, we found that van der Waals interactions and hydrophobic interactions are both important in the bonding between HoblOBP1 and hexyl benzoate. Intramolecular residues formed the hydrogen bonds in the C terminus of the protein and the bonds are crucial for the ligand-binding specificity. Finally, MET48, ILE80 and TYR111 are binding sites predicted for HoblOBP1. Using site-directed mutagenesis and fluorescence assays, it was found that ligands could not be recognized by mutant of Tyr111. A possible explanation is that the compound could not be recognized by the mutant, and remains in the binding cavity because of the loss of the intramolecular

  14. EXTRATO AQUOSO DE FOLHAS DE Azadirachta indica A. Juss NO CONTROLE DE Sitophilus zeamais Mots. (COLEOPTERA: CURCULIONIDAE EM MILHO ARMAZENADO

    Directory of Open Access Journals (Sweden)

    Marcelo Toller Borsonaro

    2013-05-01

    Full Text Available The present work had as objective to test different dosages of aqueous extract of leaves of neem(Azadirachta indica as natural alternative and of easy application in the control of Sitophilus zeamais in grains of corn, seeing it is one of main stored corn pests. Were evaluated the following concentrations: 10%, 20%, 30% and witness. They were applied directly on the mass of grains and the insects with a pressurized pulverizer model Guarany PCP-1P in the dosage of 0,56 mL for dish. The treatments did not present significant difference as the reduction of weight of grains, but there was significant difference in relation to the population control. As for this parameter, the aqueous extract of leaves of neem in concentration 30,3%, can be considered an alternative treatment against S. zeamais in stored corn grains.O presente trabalho teve como objetivo testar diferentes dosagens de extrato aquoso de folhas de Nim (Azadirachta indica A Juss como alternativa natural e de fácil aplicação no controle de Sitophilus zeamais Mots.(Coleoptera: Curculionidae em grãos de milho (Zea mays L., visto que esta é uma das principais pragas do milhoarmazenado. Avaliaram-se as seguintes concentrações: 10%, 20%, 30,3%, além do tratamento testemunha. Foram aplicados diretamente sobre a massa de grãos e sobre os insetos com um pulverizador pressurizado modelo Guarany PCP-1P na dosagem de 0,56 mL por placa. Os tratamentos não apresentaram diferença significativa quanto à redução de peso de grãos, mas houve diferença significativa em relação ao controle populacional. Quanto a este parâmetro, o extrato aquoso de folhas de Nim na concentração 30,3%, pode ser considerado um tratamento alternativo no controle de S. zeamais em grãos de milho armazenado.

  15. ABUNDÂNCIA RELATIVA DAS ESPÉCIES DE CERAMBYCIDAE (INSECTA-COLEOPTERA EM POMAR DE FRUTÍFERAS MISTO RELATIVE ABUNDANCE OF SPECIES OF THE CERAMBYCIDAE FAMILY (INSECTA-COLEOPTERA IN MIXED ORCHARD

    Directory of Open Access Journals (Sweden)

    Elisabeth Rose Pereira da Silva

    2007-09-01

    Full Text Available

    Estudou-se a abundância relativa das espécies da família Cerambycidae (Insecta-Coleoptera em um pomar de frutíferas misto composto por 28 diferentes espécies, no período de dezembro de 1997 a maio de 1999 em Ceres, Estado de Goiás, Brasil. Foram utilizadas armadilhas luminosas modelo “Luiz de Queiroz” com lâmpadas Bl-15 wats. Essas armadilhas foram ligadas por 12 horas em dois dias consectivos num total de 24 horas de coletas semanais. Coletaram-se 1.474 cerambicídeos, agrupados em 39 gêneros e 49 espécies. Acanthoderes jaspidea, Achryson surinamum, Chlorida festiva, Eurodacrys sexgutatta, Gnomibidion fulvipes, Lophopoeum timbouve, Megacyllene acuta, Rhopalophora collaris e Trichophorus distinctus foram as espécies classificadas como muito abundantes. Das espécies coletadas, 48,98 % foram classificadas como raras, 12,24 % como dispersas, 20,41 % como comuns e 18,37 % como muito abundantes. Dentre os 39 gêneros, Oreodera foi representado por três espécies (6,13 %, os gêneros Acanthoderes, Aerenica, Chrysoprasis, Colobothea, Eutrypanus, Megacylene, Myoxomorpha e Nyssodrysternum por duas espécies (4,08 %, e os demais gêneros por apenas uma espécie.

    PALAVRAS-CHAVE: Levantamento; riqueza de espécies; ecologia; comportamento.

    It was studied the relative abundance to the species of the family Cerambycidae (Insecta-Coleoptera in a mixed orchard composed by 28 diferent species in the period from decembre 1997 to may 1999 in Ceres, state of Goi

  16. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

    Science.gov (United States)

    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineensis × E. oleifera) × E. guineensis backcrosses. Females laid their eggs similarly on all breeding populations, with a preference for the E. guineensis La Mé origin. The average hatching rate reached 80% for the La Mé origin as opposed to 28% for the Deli origin. The mortality rates for the larval instars were greater on E. oleifera, on certain backcrosses and on the Deli origin of E. guineensis. Development at the second- and third- larval instars was the most affected, with a mortality rate of three to five times greater than that seen on La Mé. Epidermis and cuticle measurements indicated which breeding populations were suitable or unsuitable for the development of C. lameensis. E. guineensis, with its thin epidermis (12 µm) and cuticle (2 µm), proved to be highly susceptible to C. lameensis attacks. On the other hand, E. oleifera, which is very resistant, exhibited a thicker epidermis (17 µm) and cuticle (4 µm). The breeding populations were thus classified according to the positive or negative influence they exerted on the insect’s egg laying and feeding. RÉSUMÉ. La comestibilité de différents matériels végétaux d’Elaeis sp. présents au Bénin est testée pour Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), important ravageur du palmier à huile en Afrique. Des expérimentations en manchons ont permis de déterminer les capacités d’oviposition des femelles et les taux de mortalité des différents stades de d

  17. Resistência de genótipos de batata (Solanum spp. a Diabrotica speciosa (Germar, 1824 (Coleoptera: Chrysomelidae Resistence of potatoes genotypes (Solanum spp. to Diabrotica speciosa (Germar,1824 (Coleoptera: Chrysomelidae

    Directory of Open Access Journals (Sweden)

    Fernando Mesquita Lara

    2000-12-01

    Full Text Available Avaliou-se a resistência de genótipos de batata (Solanum spp. a Diabrotica speciosa (Germar,1824 em condições de campo e laboratório. O plantio de campo foi efetuado em julho de 1997, na FCAV/ UNESP, Campus de Jaboticabal, utilizando-se os seguintes genótipos: Achat, NYL 235-4, 288.759-16, 288. 771-6, 288.776-3, 288.776-6, 288.787-5, 288.801-8 e 288.814-7. Avaliaram-se o consumo de área foliar em levantamentos realizados aos 70 e 90 dias após plantio e os danos ocasionados pelas larvas aos tubérculos, após a colheita. Em laboratório, foram realizados testes de preferência para alimentação com plantas de 40 dias (teste sem chance de escolha, envolvendo os nove genótipos presentes no campo, e com plantas de 47 e 68 dias (com e sem chance de escolha, avaliando-se cinco genótipos. Constatou-se, no ensaio de campo, que os genótipos 288.759-16, 288.776-3, 288.776-6, 288.787-5 e 288.814-7 foram menos consumidos por adultos de D. speciosa que a cultivar Achat, porém, em laboratório, 288.759-16 e 288.776-6 não rev elaram essa característica. O consumo em NYL 235-4 e 288.801-8 foi intermediário, não diferindo dos demais. NYL 235-4 e 288.801-8 foram resistentes às larvas, apresentando, em média, menos que 3 furos por tubérculo. O genótipo 288.776-3 destacou-se como resistente a larvas e adultos de D. speciosa, sendo a resistência a adultos do tipo não-preferência para alimentação (antixenose.The resistance of potato genotypes to Diabroticaspeciosa (Germar,1824 (Coleoptera: Chrysomelidae, was evaluated under field and laboratory conditions. The field trial was set up in the experimental farm of FCAV - UNESP, Jaboticabal, SP, and the following genotypes were compared: Achat, NYL 235-4, 288.759-16, 288.771-6, 288.776-3, 288.776-6, 288.787-5, 288.801-8 e 288.814-7. The leaf area consumption by adults was evaluated at 70 and 90 days after planting, and, after harvest, larval damage to tubers (number of perforations was assessed

  18. Systematics and phylogenetics of Indo-Pacific Luciolinae fireflies (Coleoptera: Lampyridae) and the description of new genera.

    Science.gov (United States)

    Ballantyne, Lesly A; Lambkin, Christine L

    2013-01-01

    This revision completes a taxonomic survey of fireflies (Coleoptera: Lampyridae) in the area encompassed by Australia, the Republic of Palau, Federated States of Micronesia, Papua New Guinea, Indonesia (West Irian/Papua), Solomon Islands, New Caledonia, Vanuatu and Fiji. It finalises the taxonomic issues arising from the 1969–70 voyage of the scientific vessel Alpha Helix to New Guinea. The firefly fauna of this area is exclusively Luciolinae. The scope of the revision was extended to include all known Luciolinae genera and certain species from SE Asia, and a phylogenetic analysis of 436 morphological characters of males, females, and associated larvae includes 142 Luciolinae species (Ballantyne & Lambkin 2009, and Fu et al. 2012a). The phylogenetic analyses infer four major groups within the Luciolinae. The monotypic Missimia Ballantyne is sister to all remaining Luciolinae and forms a grade to Aquatica Fu etBallantyne. The large clade of Curtos Motschulsky, Photuroluciola Pic, Colophotia Motschulsky, Poluninius gen. nov., Pyrophanes Olivier, Pteroptyx s. str. Olivier, Medeopteryx gen. nov., Trisinuata gen. nov., and Australoluciola gen. nov.forms a grade to the clade of Luciola s. str. Laporte (including Bourgeoisia Olivier). The monotypic Emeia Fu et al.forms a grade with a clade of Luciola and Pygoluciola Wittmer, sister to a large clade of Convexa Ballantyne, Pacifica gen. nov., Magnalata Ballantyne, Lloydiella Ballantyne, Asymmetricata Ballantyne, Pygatyphella s. str. Ballantyne, Atyphella Olliff, Aquilonia Ballantyne, and Gilvainsula Ballantyne. Luciola is paraphyletic, found in up to six clades across the tree. Together with Luciola, Magnalata, Aquilonia, and Gilvainsula render Atyphella paraphyletic. The new genera described here are all monophyletic and supported in the phylogenetic analyses that also provide evidence for the inclusion of taxa within them. Twenty-three genera including five new ones, and ten new species, are recognised and keys are

  19. Střevlíkovití a drabčíkovití (Coleoptera, Staphylinidae) brouci NPR Brouskův mlýn a jejich využití pro biomonitorování stavu biotopů

    Czech Academy of Sciences Publication Activity Database

    Boháč, Jaroslav

    České Budějovice : Calla, 2003, s. 14-18. [Národní přírodní rezervace Brouskův mlýn. Borovany (CZ), 06.06.2003] R&D Projects: GA AV ČR KSK6005114 Keywords : Coleoptera * Staphylinidae * biomonitoring Subject RIV: EH - Ecology, Behaviour

  20. Distribución y caracterización morfológica de Hycleus duodecimmaculatus (Olivier, 1811 (Coleoptera, Meloidae, una especie poco conocida del oeste de Marruecos

    Directory of Open Access Journals (Sweden)

    Ruiz, José L.

    2014-06-01

    Full Text Available Hycleus duodecimmaculatus (Coleoptera, Meloidae, Mylabrini is a poorly known species endemic to Morocco. Its geographic range extends along the coastal-subcoastal fringe of the Atlantic Coast, between Larache and Tiznit region (Arbaa Ait Ahmed. In this paper we study more than a hundred specimens, mostly preserved in the collection of the Museo Nacional de Ciencias Naturales (MNCN-CSIC, Spain. We provide new geographic records, including the Western Rif and confirm its presence in Marrakech (northwestern slopes of the High Atlas. Because of the scarcity of information, disperse and sometimes erroneous, we synthesize information available on chorology and phenology, and characterize the typology of the species’ habitat. From a taxonomic point of view, we redefine the species’ diagnostic characters and illustrate various aspects of the intra- and interpopulational morphological variability.Hycleus duodecimmaculatus (Coleoptera, Meloidae, Mylabrini es un endemismo de Marruecos muy poco conocido, cuya distribución se extiende por la franja costera-subcostera del Marruecos atlántico, entre Larache y la región de Tiznit (Arbaa Ait Ahmed. En este trabajo, realizado principalmente a partir del estudio de más de un centenar de ejemplares conservados en su mayor parte en la colección del Museo Nacional de Ciencias Naturales (MNCN-CSIC, España, se aportan nuevas citas geográficas incluyendo el Rif occidental, y se confirma su presencia en Marrakech (falda noroccidental del Alto Atlas. A la vista de la escasez de información existente para esta especie, dispersa y a veces errónea, se sintetiza la información corológica disponible, y se ofrecen datos sobre fenología de los adultos y tipología general de los hábitats que ocupa. Desde el punto de vista taxonómico, se redefinen los caracteres diagnósticos de la especie y se ilustran diversos aspectos de la variabilidad morfológica intra e interpoblacional.

  1. Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae in the boreal balsam fir forest of Quebec, Canada

    Directory of Open Access Journals (Sweden)

    Jan Klimaszewski

    2013-11-01

    Full Text Available Experimental research on beetle responses to removal of logging residues following clearcut harvesting in the boreal balsam fir forest of Quebec revealed several abundant rove beetle (Staphylinidae species potentially important for long-term monitoring. To understand the trophic affiliations of these species in forest ecosystems, it was necessary to analyze their gut contents. We used microscopic and molecular (DNA methods to identify the gut contents of the following rove beetles: Atheta capsularis Klimaszew­ski, Atheta klagesi Bernhauer, Oxypoda grandipennis (Casey, Bryophacis smetanai Campbell, Ischnosoma longicorne (Mäklin, Mycetoporus montanus Luze, Tachinus frigidus Erichson, Tachinus fumipennis (Say, Tachinus quebecensis Robert, and Pseudopsis subulata Herman. We found no apparent arthropod fragments within the guts; however, a number of fungi were identified by DNA sequences, including filamentous fungi and budding yeasts [Ascomycota: Candida derodonti Suh & Blackwell (accession number FJ623605, Candida mesenterica (Geiger Diddens & Lodder (accession number FM178362, Candida railenensis Ramirez and Gonzáles (accession number JX455763, Candida sophie-reginae Ramirez & González (accession number HQ652073, Candida sp. (accession number AY498864, Pichia delftensis Beech (accession number AY923246, Pichia membranifaciens Hansen (accession number JQ26345, Pichia misumaiensis Y. Sasaki and Tak. Yoshida ex Kurtzman 2000 (accession number U73581, Pichia sp. (accession number AM261630, Cladosporium sp. (accession number KF367501, Acremonium psammosporum W. Gams (accession number GU566287, Alternaria sp. (accession number GU584946, Aspergillus versicolor Bubak (accession number AJ937750, and Aspergillus amstelodami (L. Mangin Thom and Church (accession number HQ728257]. In addition, two species of bacteria [Bradyrhizobium japonicum (KirchnerJordan (accession number BA000040 and Serratia marcescens Bizio accession number CP003942] were found in

  2. Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada.

    Science.gov (United States)

    Klimaszewski, Jan; Morency, Marie-Josee; Labrie, Philippe; Séguin, Armand; Langor, David; Work, Timothy; Bourdon, Caroline; Thiffault, Evelyne; Paré, David; Newton, Alfred F; Thayer, Margaret K

    2013-01-01

    Experimental research on beetle responses to removal of logging residues following clearcut harvesting in the boreal balsam fir forest of Quebec revealed several abundant rove beetle (Staphylinidae) species potentially important for long-term monitoring. To understand the trophic affiliations of these species in forest ecosystems, it was necessary to analyze their gut contents. We used microscopic and molecular (DNA) methods to identify the gut contents of the following rove beetles: Atheta capsularis Klimaszewski, Atheta klagesi Bernhauer, Oxypoda grandipennis (Casey), Bryophacis smetanai Campbell, Ischnosoma longicorne (Mäklin), Mycetoporus montanus Luze, Tachinus frigidus Erichson, Tachinus fumipennis (Say), Tachinus quebecensis Robert, and Pseudopsis subulata Herman. We found no apparent arthropod fragments within the guts; however, a number of fungi were identified by DNA sequences, including filamentous fungi and budding yeasts [Ascomycota: Candida derodonti Suh & Blackwell (accession number FJ623605), Candida mesenterica (Geiger) Diddens & Lodder (accession number FM178362), Candida railenensis Ramirez and Gonzáles (accession number JX455763), Candida sophie-reginae Ramirez & González (accession number HQ652073), Candida sp. (accession number AY498864), Pichia delftensis Beech (accession number AY923246), Pichia membranifaciens Hansen (accession number JQ26345), Pichia misumaiensis Y. Sasaki and Tak. Yoshida ex Kurtzman 2000 (accession number U73581), Pichia sp. (accession number AM261630), Cladosporium sp. (accession number KF367501), Acremoniumpsammosporum W. Gams (accession number GU566287), Alternaria sp. (accession number GU584946), Aspergillus versicolor Bubak (accession number AJ937750), and Aspergillusamstelodami (L. Mangin) Thom and Church (accession number HQ728257)]. In addition, two species of bacteria [Bradyrhizobium japonicum (Kirchner) Jordan (accession number BA000040) and Serratia marcescens Bizio accession number CP003942] were found in

  3. FACTORES QUE INFLUYEN EN LA ABUNDANCIA DE LARVAS DE COLEOPTERA: MELOLONTHIDAE CON IMPORTANCIA AGRÍCOLA EN LA REGIÓN PURHÉPECHA, MICHOACÁN, MÉXICO

    OpenAIRE

    Esperanza Pérez-Agis; Miguel Ángel Morón; Miguel B. Nájera-Rincón; Adriana E. Castro-Ramírez

    2014-01-01

    Para definir el nivel de influencia de los factores ambientales y del manejo del agroecosistema en el complejo de larvas de Coleoptera Melolonthidae en dos localidades productoras de maíz en la Región Purhépecha de Michoacán, México, una con manejo extensivo (San Felipe) y la otra con manejo intensivo (Uricho), se seleccionaron cuatro tipos de factores que pueden influir en su distribución: i) físicos, ii) manejo de la fertilidad del suelo, iii) biológicos, y iv) temperatura y precipitación d...

  4. Taxonomia dos bruquíneos associados à Senna Neglecta (Vogel) H. S. Irwin e Barneby (Fabaceae: Caesalpinioideae), com revisão do grupo Abbreviatus de Sennis Bridwell (Coleoptera, Chrsomelidae, Bruchinae)

    OpenAIRE

    Viana, Jéssica Herzog Viana

    2011-01-01

    Resumo: Senna neglecta (Vogel) H. S. Irwin & Barneby ocorre nas regiões Nordeste, Sudeste e Sul do Brasil, em campos, vegetação secundária, borda e interior de mata. É uma planta estudada para uso em paisagismo, recuperação de áreas degradadas e produção de fármacos antimicrobiais. Um estudo taxonômico foi realizado com as espécies de Bruchinae (Chrysomelidae: Coleoptera) associadas à esta planta a partir de caracteres morfológicos do exoesqueleto e genitália masculina e feminina. Após levant...

  5. Larvae and pupae of two North American darkling beetles (Coleoptera, Tenebrionidae, Stenochiinae, Glyptotus cribratus LeConte and Cibdelis blaschkei Mannerheim, with notes on ecological and behavioural similarities

    Directory of Open Access Journals (Sweden)

    Warren Steiner

    2014-06-01

    Full Text Available This study describes and illustrates the larvae and pupae of two North American darkling beetles (Coleoptera: Tenebrionidae in the subfamily Stenochiinae, Glyptotus cribratus LeConte from the southeastern United States, and Cibdelis blaschkei Mannerheim from California. Both species inhabit forested regions where adults and larvae occur in soft rotten dry wood of dead branches on living trees or in sections recently fallen from them. Species identity was confirmed by rearing of adults and pupae and the discovery of both in pupal cells with associated exuvia. Specimen label data and notes on habitats are provided. Antipredator defense structures and behaviour are noted for larvae and pupae of both species.

  6. Gyriosomus granulipennis Pizarro-Araya & Flores 2004 (Coleoptera: Tenebrionidae): An extreme case to preserve Gyriosomus granulipennis Pizarro-Araya & Flores 2004 (Coleóptera: Tenebrionidae): Un caso extremo a conservar

    OpenAIRE

    JAIME PIZARRO-ARAYA; OLIVIA E VERGARA; Gustavo E. Flores

    2012-01-01

    The Choros Archipelago includes three islands: Choros, Damas and Gaviota and it is part of The Pingüino de Humboldt National Reserve. These insular ecosystems are within the Chilean transitional coastal (25°-32° S). Prior researches in the archipelago reported a species of Tenebrionidae (Coleoptera) endemic to Choros island (29°15' S, 71°32' W) described as Gyriosomus granulipennis Pizarro-Araya & Flores. The objective of this paper is to evaluate the vulnerability of this species in the arch...

  7. Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae)

    Institute of Scientific and Technical Information of China (English)

    YANG Pu; ZHOU WenWu; ZHANG Qin; CHENG JiaAn; ZHU ZengRong; WAY M O

    2009-01-01

    The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidee), reproduces by sex in the Southeastern United States, but reproduces by parthenogenesis in California and other in-vaded regions in Asia and Europe. The objective of this study was to create a parthenogenetic gene expression profile of the rice water weevil in order to gain a better insight into the molecular mecha-nisms of parthenogenesis in the weevil. Suppression subtractive hybridization (SSH) technique was employed for profiling differential gene expression in the developed ovary between the parthenoge-netic and bisexual female rice water weevils. A total of 70 contigs were obtained, and the BLASTX search identified putatively 28 genes with differential functions. According to the cytological process of parthenogenesis, the tubulin alpha-1 chain and signal transduction genes etc. were selected for real time quantitative RT-PCR analyses, and their possible functions related to the molecular mechanism of parthenogenesis were discussed. The tubulin alpha-1 chain and some signal transduction genes may be related to the molecular mechanisms of parthenogenesis of the rice water weevil.

  8. The invasion history, distribution and colour pattern forms of the harlequin ladybird beetle Harmonia axyridis (Pall. (Coleoptera, Coccinellidae in Slovakia, Central Europe

    Directory of Open Access Journals (Sweden)

    Ľubomír Panigaj

    2014-05-01

    Full Text Available The harlequin ladybird beetle Harmonia axyridis (Coleoptera, Coccinellidae has invaded and established in Slovakia. Following unintentional introduction in 2008, the spread of the alien coccinellid was very fast. By the end of 2009, it was recorded across the whole country, and by the end of 2012 it was widely distributed and common in various habitats, particularly gardens, orchards and urban areas, where it was most frequent on trees. The rate of eastward spread was approximately 200 km year-1, similar to the overall rate of spread in Europe. Between 2008 and 2012, the coccinellid was recorded in a total of 153 localities, in altitudes ranging from 98 to 1,250 m. Most records of this species were made in lowlands, hilly areas and valleys separating mountain ridges. However, it was only rarely documented in areas above 700 m a.s.l. The non-melanic colour form (f. succinea was dominant along alongitudinal transect including eight urban areas across Slovakia, with the frequency of melanic forms (f. spectabilis and f. conspicua together between 6.3 and 19.2% and a median equal to 10.5%. The invasion history and distribution of H. axyridis in Slovakia are discussed with regard to the time sequence of records, rate of spread, altitudinal distribution, anthropogenic dispersal, effective recording, proportion of melanic forms and other relevant aspects associated with the spread of this successful invader.

  9. Evaluation of Agrilus planipennis (Coleoptera: Buprestidae) control provided by emamectin benzoate and two neonicotinoid insecticides, one and two seasons after treatment.

    Science.gov (United States)

    McCullough, Deborah G; Poland, Therese M; Anulewicz, Andrea C; Lewis, Phillip; Cappaert, David

    2011-10-01

    Effective methods are needed to protect ash trees (Fraxinus spp.) from emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive buprestid that has killed millions of North American ash (Fraxinus spp.) trees. We randomly assigned 175 ash trees (11.5-48.1 cm in diameter) in 25 blocks located in three study sites in Michigan to one of seven insecticide treatments in May 2007. Treatments included 1) trunk-injected emamectin benzoate; 2) trunk-injected imidacloprid; 3) basal trunk spray of dinotefuran with or 4) without Pentra-Bark, an agricultural surfactant; 5) basal trunk spray of imidacloprid with or 6) without Pentra-Bark; or (7) control. Foliar insecticide residues (enzyme-linked immunosorbent assay) and toxicity of leaves to adult A. planipennis (4-d bioassays) were quantified at 3-4-wk intervals posttreatment. Seven blocks of trees were felled and sampled in fall 2007 to quantify A. planipennis larval density. Half of the remaining blocks were retreated in spring 2008. Bioassays and residue analyses were repeated in summer 2008, and then all trees were sampled to assess larval density in winter. Foliage from emamectin benzoate-treated trees was highly toxic to adult A. planipennis, and larval density was or = 2 yr may reduce costs or logistical issues associated with treatment. PMID:22066190

  10. Diversity and community structure of dung beetles (Coleoptera: Scarabaeidae across a habitat disturbance gradient in Lore Lindu National Park, Central Sulawesi

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    SHAHABUDDIN

    2010-01-01

    Full Text Available Shahabuddin (2010 Diversity and community structure of dung beetles (Coleoptera: Scarabaeidae across habitat disturbance gradient in Lore Lindu National Park, Central Sulawesi. Biodiversitas 11: 29-33. Dung beetles are important component of most terrestrial ecosystems and used to assess the effects of habitat disturbance and deforestation. This study aimed at comparing dung beetle assemblages among several habitat types ranging from natural tropical forest and agroforestry systems to open cultivated areas at the margin of Lore Lindu National Park (LLNP, Central Sulawesi (one of Indonesia’s biodiversity hotspots. Therefore, 10 pitfall traps baited with cattle dung were exposed at each habitat type (n = 4 replicate sites per habitat type to collect the dung beetles. The results showed that species richness of dung beetles declined significantly from natural forest to open area. However cacao agroforestry systems seemed to be capable of maintaining a high portion of dung beetle species inhabiting at forest sites. The closer relationship between dung beetle assemblages recorded at forest and agroforestry sites reflects the high similarity of some measured habitat parameters (e.g. vegetation structure and microclimate between both habitat types, while species assemblages at open areas differed significantly from both other habitat groups. These results indicated that habitat type has importance effect on determining the species richness and community structure of dung beetles at the margin of LLNP.

  11. Residual efficacy of methoprene for control of Tribolium castaneum (Coleoptera: Tenebrionidae) larvae at different temperatures on varnished wood, concrete, and wheat.

    Science.gov (United States)

    Wijayaratne, L K Wolly; Fields, Paul G; Arthur, Frank H

    2012-04-01

    The residual efficacy of the juvenile hormone analog methoprene (Diacon II) was evaluated in bioassays using larvae of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) exposed on unsealed concrete or varnished wood treated with a liquid formulation and held at different temperatures. When these two types of surfaces were stored at 20, 30 or 35 degrees C for 0-24 wk, the percentage of adult emergence on concrete increased with time. In contrast, there was no adult emergence from larvae exposed to varnished wood at 24 wk after treatment at any of these temperatures. The presence of flour reduced residual efficacy of methoprene on concrete, but not on varnished wood, with no differences between cleaning frequencies. Methoprene was also stable for 48 h on concrete held at 65 degrees C and wheat, Triticum aestivum L., held at 46 degrees C. Results show that methoprene is stable at a range of temperatures commonly encountered in indoor food storage facilities and at high temperatures attained during insecticidal heat treatments of structures. The residual persistence of methoprene applied to different surface substrates may be affected more by the substrate than by temperature. PMID:22606845

  12. Escarabeíneos copro-necrófagos (Coleoptera, Scarabaeidae, Scarabaeinae de fragmentos de Mata Atlântica em Silveira Martins, Rio Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Pedro G. da Silva

    2012-06-01

    Full Text Available A fauna de Scarabaeinae (Coleoptera: Scarabaeidae foi amostrada através de armadilhas de queda iscadas com excremento humano e peixe apodrecido em fragmentos florestais de Silveira Martins, Rio Grande do Sul, Brasil, de novembro de 2010 a janeiro de 2011. Foi coletado um total de 1.611 indivíduos, pertencentes a seis tribos, 11 gêneros e 28 espécies. As espécies mais abundantes foram Canthon latipes Blanchard, 1845 (49,9%, C. chalybaeus Blanchard, 1845 (13,9%, Deltochilum sculpturatum Felsche, 1907 (4,9% e Eurysternus caribaeus (Herbst, 1789 (4,3%, que juntas representaram 73% do total de indivíduos capturados. As armadilhas iscadas com excremento humano capturaram maior número de espécies do que as iscadas com peixe apodrecido. Não houve diferença estatística significativa entre os tipos de iscas utilizados em relação à abundância de Scarabaeinae. A maior parte da comunidade de Scarabaeinae capturada foi representada por espécies de hábito alimentar generalista e comportamento escavador. A comunidade amostrada segue os padrões gerais de estrutura trófica e comportamental de Scarabaeinae encontrados por toda a região Neotropical.

  13. The SNMP/CD36 gene family in Diptera, Hymenoptera and Coleoptera: Drosophila melanogaster, D. pseudoobscura, Anopheles gambiae, Aedes aegypti, Apis mellifera, and Tribolium castaneum.

    Science.gov (United States)

    Nichols, Zachary; Vogt, Richard G

    2008-04-01

    Sensory neuron membrane proteins (SNMPs) are membrane bound proteins initially identified in olfactory receptor neurons of Lepidoptera and are thought to play a role in odor detection; SNMPs belong to a larger gene family characterized by the human protein CD36. We have identified 12-14 candidate SNMP/CD36 homologs from each of the genomes of Drosophila melanogaster, D. pseudoobscura, Anopheles gambiae and Aedes aegypti (Diptera), eight candidate homologs from Apis mellifera (Hymenoptera), and 15 from Tribolium castaneum (Coleoptera). Analysis (sequence similarity and intron locations) suggests that the insect SNMP/CD36 genes fall into three major groups. Group 1 includes the previously characterized D. melanogaster emp (epithelial membrane protein). Group 2 includes the previously characterized D. melanogaster croquemort, ninaD, santa maria, and peste. Group 3 genes include the SNMPs, which fall into two subgroups referred to as SNMP1 and SNMP2. D. melanogaster SNMP1 (CG7000) shares both significant sequence similarity and five of its six intron insertion sites with the lepidopteran Bombyx mori SNMP1. The topological conservation of this gene family within the three major holometabolous lineages indicates that it predates the coleopteran and hymenoptera/dipera/lepidoptera split 300+ million years ago. The current state of knowledge of the characterized insect members of this gene family is discussed. PMID:18342246

  14. Plant vigor metrics determine spatio-temporal distribution dynamics of Oulema melanopus (Coleoptera: Chrysomelidae) and its larval parasitoid, Tetrastichus julis (Hymenoptera: Eulophidae).

    Science.gov (United States)

    Kher, S V; Dosdall, L M; Cárcamo, H A

    2014-10-01

    The cereal leaf beetle, Oulema melanopus (L.) (Coleoptera: Chrysomelidae), is a new invasive insect pest of oat, wheat, and barley in western Canada. Biological control with its principal larval parasitoid, Tetrastichus julis Walker (Hymenoptera: Eulophidae), is the primary management strategy. However, to implement control successfully, a thorough understanding of the spatio-temporal dynamics of the interactions between these two species is important. We examined the nature of spatial associations and distribution dynamics of O. melanopus and T. julis with reference to host plant nutrients and plant vigor traits using Spatial Analysis by Distance Indices. A grid design was used to understand spatial associations between O. melanopus and T. julis. Distributions of O. melanopus and T. julis indicated the presence of significant patches and gaps. Plant nutrient availability and plant vigor varied across the grid in all study years. On a spatial scale, O. melanopus and T. julis represented a tightly coupled system demonstrating the strong density-dependent nature of parasitoid dispersal. Among the factors examined, plant vigor traits significantly influenced field distributions of both O. melanopus and T. julis. Areas across grids with high plant density, greater plant height, and high availability of plant leaves indicated higher establishment of O. melanopus larvae, consequently exhibiting bottom-up effects on T. julis distributions. Maintenance of uniform plant vigor can be a critical aspect in mitigating yield losses from O. melanopus infestation. PMID:25259693

  15. Improving detection tools for emerald ash borer (Coleoptera: Buprestidae): comparison of multifunnel traps, prism traps, and lure types at varying population densities.

    Science.gov (United States)

    Crook, Damon J; Francese, Joseph A; Rietz, Michael L; Lance, David R; Hull-Sanders, Helen M; Mastro, Victor C; Silk, Peter J; Ryall, Krista L

    2014-08-01

    The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a serious invasive pest of North American ash (Fraxinus spp.) that has caused devastating mortality since it was first identified in North America in 2002. In 2012, we conducted field trapping assays that tested the efficacy of purple prism and fluon-coated green multifunnel (Lindgren funnel) traps. Traps were baited with combinations of several lures that were previously shown to be attractive to A. planipennis: manuka oil--a sesquiterpene-rich oil, (3Z)-hexenol--a green leaf volatile, or (3Z)-dodecen-12-olide [= (3Z)-lactone], a sex pheromone. Eighty-nine blocks (trap lines) were tested throughout nine states along the outer edges of the currently known A. planipennis infestation in North America. Trap catch was highest on fluon-coated green multifunnel traps, and trap detections at sites with low A. planipennis population density ranged from 72 to 76% for all trap and lure types tested. (3Z)-hexenol and (3Z)-lactone baited traps functioned as well as (3Z)-hexenol and manuka oil-baited traps. Independent of the lure used, detection rates on green fluon-coated multifunnel traps were comparable with glued purple prism traps in areas with low A. planipennis population densities. PMID:25195441

  16. FLUTUAÇÃO POPULACIONAL DE Triozoida limbata ENDERLEIN (HEMIPTERA: TRIOZIDAE E DE Scymnus spp. (COLEOPTERA: COCCINELLIDAE EM POMAR DE GOIABA (Psidium guajava L.

    Directory of Open Access Journals (Sweden)

    Rogério Teixeira Duarte

    2015-09-01

    Full Text Available In order to analyze the contribution of the natural biological control of the coccinellid predator Scymnus spp. (Coleoptera: Coccinellidae on Triozoida limbata Enderlein (Hemiptera: Sternorrhyncha: Triozidae, the aim of this research was to study the populational fluctuation of these arthropods in semi-organic orchard of guava and correlate the populational density of this pest with meteorological elements. We used yellow stick traps for monitoring adult of T. limbata and Scymnus spp., spaced approximately 30 m from each other at a height of 1.5 m above the ground, inside random plants. These traps were maintained in the field for 15 days and replaced by new traps, without interruption, totaling 23 samples. We calculated the Pearson correlation coefficients (r to correlate the populational density of T. limbata with populational fluctuation of Scymnus spp., and meteorological elements. We also made histograms with information about the populational fluctuation of the specimens sampled and temperature (°C and precipitation (mm values. The highest densities of adults of T. limbata occurred almost between spring and summer seasons. The populational density of Scymnus spp. was positively correlated with populational density of T. limbata. There was no correlation between the populational fluctuation of T. limbata with meteorological elements.

  17. Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion

    Indian Academy of Sciences (India)

    Amanda Paulino De Arcanjo; Diogo Cavalcanti Cabral-De-Mello; Ana Emília Barros E Silva; Rita De Cássia De Moura

    2009-08-01

    Mitotic and meiotic chromosomes of several populations of Eurysternus caribaeus (Coleoptera: Scarabaeidae) were analysed through conventional staining, C-banding, base-specific fluorochromes, silver nitrate staining and fluorescent in situ hybridization (FISH). All specimens showed $2n = 8$ in their karyotypes, with a neo-XY sex system (Y is a submetacentric and X a metacentric) and three pairs of submetacentric autosomes. The analysis of constitutive heterochromatin (CH) revealed small blocks located in the centromeric region of all chromosomes which do not present positive staining under the fluorochromes CMA3 and DAPI. Silver nitrate staining revealed that the nucleolar organizer region (NORs) is associated with the sex chromosomes. The FISH technique revealed that rDNA sites in the X and Y are different in size. Data from different populations indicate that the diploid number reduction $(2n = 8)$ observed in E. caribaeus is established and presumably has preceded the dispersion of this species. Moreover, this reduction occasioned the translocation of rDNA sites to the sex chromosomes, X and Y, an uncommon pattern in Scarabaeidae that was observed for the first time by the FISH in this work.

  18. The night and day of dung beetles (Coleoptera, Scarabaeidae in the Serra do Japi, Brazil: elytra colour related to daily activity

    Directory of Open Access Journals (Sweden)

    Malva Isabel Medina Hernández

    2002-01-01

    Full Text Available In the present study 387 dung beetles (Coleoptera: Scarabaeidae were surveyed at the Serra do Japi, in the Atlantic Forest in São Paulo State, with four baited pitfall traps during the months of December, 1998, and January, 1999 during eight 24 hour cycles. A total of 30 species were identified and temporal variation in activity patterns among the species shows a specialization in the use of food resources: 9 species were classified as nocturnal and 13 as diurnal. The daily activity pattern of dung beetles does not necessarily correspond to the taxonomic classification, but is strongly related to the colouring of species, determined by predominant elytra colour: nocturnal species have 89 % more chances of being black as opposed to colourful. Black nocturnal species might have evolved as an interspecific adaptation to avoid predation (cryptic colouring. Among the colourful diurnal dung beetles, measure of body length of each species shows that development of bright colouring was more often found in medium to large species, which suggests that colouring evolved as a response to intraspecific pressures, important in agonistic encounters among males.

  19. Concentration-dependent effects of GABA on insensitivity to fipronil in the A2'S mutant RDL GABA receptor from fipronil-resistant Oulema oryzae (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Nakao, Toshifumi; Naoi, Atsuko; Hama, Masako; Kawahara, Nobuyuki; Hirase, Kangetsu

    2012-10-01

    The beetle Oulema oryzae Kuwayama (Coleoptera: Chrysomelidae), an important pest of rice, has developed fipronil resistance in Japan. Molecular cloning and sequence analysis of O. oryzae RDL gamma-aminobutyric acid (GABA) receptor subunit (OO-RDL) genes from fipronil-susceptible and -resistant O. oryzae identified the A2'S mutation (index number for the M2 membrane-spanning region). To investigate the effect of the A2'S mutation on fipronil resistance, we stably expressed the wild-type and mutant OO-RDL homomers in Drosophila Mel-2 cells. A membrane potential assay exhibited that the IC50 values of fipronil for inhibition of the response to EC80 GABA of the wild-type and A2'S mutant OO-RDL homomers were 0.09 microM and 0.11 microM, respectively. However, the IC50 values of fipronil for inhibition of the response to EC95 GABA of the wild-type and A2'S mutant OO-RDL homomers were 0.11 microM and approximately equal to 5 microM, respectively. These results suggest that the GABA concentration is an important factor affecting fipronil resistance in O. oryzae carrying the A2'S mutation in OO-RDL. PMID:23156177

  20. Fauna de Coleoptera Associada a Carcaças de Coelhos Expostas em uma Área Urbana no Sul do Brasil

    Directory of Open Access Journals (Sweden)

    Richard Silva

    2012-11-01

    Abstract. Study of Coleoptera fauna associated with rabbit carcasses, Oryctolagus cuniculus Linnaeus (Lagomorpha, Leporidae, during the summer of 2010 and winter of 2011, is present at an urban area of Northern Paraná State, Southern Brazil. To capture the beetles were used pan, pitfall and modified Shannon traps. We collected 236 beetles belonging to 12 families and at least 25 species. Staphylinidae (52.5% and Histeridae (26.7% were the most abundant families in both seasons represented mainly by Aleochara bonariensis Lynch and Euspilotus "group" azureus sp., respectively. Among the seasons, both the decomposition of the carcasses and the succession of coleopterofauna of forensic interest occurred differently due to significant variation of abiotic factors. During the winter, most individuals of Euspilotus "group" azureus sp. (96.8% was captured at the stage of Black Putrefaction, showing an association at this stage. Despite the environment seasonality, there wasn’t significant difference of the abundance among seasons by main beetles neither by coleopterofauna.