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Sample records for ao triatoma sordida

  1. Interacción entre poblaciones de Triatoma infestans y Triatoma sordida Interação entre população de Triatoma infestans e Triatoma sordida Population interactions between Triatoma infestans and Triatoma sordida

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    Maria Esther Bar

    1994-02-01

    Full Text Available Para conocer si se producía algún tipo de interacción entre poblaciones de Triatoma infestans y Triatoma sordida que convivían en una unidad experimental y explotaban el mismo recurso alimentario (ave se las estudió desde setiembre/1988 a abril/1989. La composición etaria inicial para cada especie fue: 27 N1, 7 N2, 11 N3, 3 N4, 8 N5, 4 machos y 10 hembras. La dinámica de población, el estado nutricional, la predación y la conducta gregaria, utilizados como parámetros de comparación, fueron estimados mediante censos mensuales. Triatoma infestans logró mayor éxito colonizador que T. sordida dado los valores alcanzados en: fecundidad (146 huevos/hembra, longevidad (157,8 días y mortalidad (39,4%, en comparación a los obtenidos por T. sordida: 118 huevos/hembra, 81,1 días y 54,0% respectivamente. La población de T. infestans tuvo un crecimiento de tipo exponencial, con una alta tasa de renovación ninfal, en contraposición, la población de T. sordida se extinguió tempranamente. En general, el peso promedio de los ejemplares de T. infestans se mantuvo en valores próximos al inicial, mientras que en T. sordida se redujo. Los grupos gregarios se conformaron mayoritariamente en el sector inferior de la pared 1 (próximo al hospedador, observándose mayor nivel de contagio en T. infestans. A partir de los resultados obtenidos se formula la hipótesis de la superioridad competitiva en T. infestans.Para saber se algum tipo de interação se produzia entre populações de Triatoma infestans e T. sordida que conviviam em uma unidade experimental e compartilharam o mesmo recurso alimentar (ave, foi realizado estudo que abrangeu o período de setembro de 1988 a abril de 1989. A composição etária inicial para cada espécie foi: 27 N1, 7 N2, 11 N3, 3 N4, 8 N5, 4 machos e 10 fêmeas. A dinâmica da população, o estado nutricional, a predação e a conduta gregria foram estimados mediante censos mensais. T. infestans obteve maior êxito

  2. Aspectos ecológicos da tripanossomíase americana: VII. Permanência e mobilidade do Triatoma sordida em relação aos ecótopos artificiais Ecological aspects of South American Tripanosomiasis: VII. Permanence and mobility of Triatoma sordida with relation to artificial ecotopes

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    Oswaldo Paulo Forattini

    1975-12-01

    Full Text Available Nas colônias desenvolvidas espontaneamente em ecótopos artificiais, representados por galinheiros experimentais, procurou-se observar a permanência e a mobilidade de Triatoma sordida. Para tanto, procedeu-se à marcação individual de adultos e ninfas de 4.° e 5.° estádios, bem como à instalação de abrigos ao redor daqueles biótopos. Os dados obtidos permitiram observar a curta permanência dos primeiros na colônia, não ultrapassando o período de dois meses para a maioria dessas formas. A mobilidade evidenciou-se pela presença de ovos e ninfas de 1.° estádio, nos esconderijos instalados, e as evidências sugeriram ser devidas à passagem dos adultos por esses ecótopos. Concluiu-se que esta espécie é dotada de elevada capacidade de disseminação, em concordância com sua apreciável valência ecológica.Spontaneous colonies of Triatoma sordida developed in experimental fowlhouses were submitted to observations as regards permanence and mobility of this bug. During thirteen months, adults and nymphs of fourth and fifth in stars were tagged for recapture. Resting traps for atracting bugs were placed around the fowlhouses. Adults showed no more than two months of permanence inside these artificial ecotopes. Eggs and first nymphs found in the resting traps suggested activity of adults coming from the fowlhouses. It was evident that this triatomid has a dispersal capacity and an adult tendency towards leaving the colonies as soon as the first egg batches are layed. After this the female will continue the oviposition inside other ecotopes attempting to develop new colonies. This aspect agress with the high characteristic ecological valence of this bug.

  3. Monitoramento da suscetibilidade de populações de Triatoma sordida Stål, 1859 (Hemiptera: Reduviidae ao inseticida deltametrina, na região Centro-Oeste do Brasil Monitoring the susceptibility of Triatoma sordida Stål, 1859 (Hemiptera: Reduviidae to deltamethrin insecticide, in Central-Western Brazil

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    Marcos Takashi Obara

    2011-04-01

    Full Text Available INTRODUÇÃO: Populações de Triatoma sordida Stål, 1859 foram investigadas quanto à suscetibilidade à deltametrina. MÉTODOS: Análise por meio de bioensaios por aplicação tópica em 11 populações de T. sordida procedentes dos Estados de Goiás, Mato Grosso e Mato Grosso do Sul. RESULTADOS: As estimativas de DL50 e RR50 demonstraram elevados níveis de suscetibilidade (DL50 INTRODUCTION: Triatoma sordida Stål, 1859 populations were investigated for susceptibility to deltamethrin. METHODS: This study aimed to analyze the resistance of 11 populations of insects from the States of Goiás, Mato Grosso and Mato Grosso do Sul by topical application bioassays. RESULTS: The estimated LD50 and RR50 showed high levels of susceptibility (LD50 < 1 and RR50 < 2. However, analysis of the slope of the dose response curve showed that the populations of insects in the towns of Firminópolis/GO, Posse/GO, Poxoréu/MT, Douradina/MS and Aparecida do Taboado/MS present great probability of evolving resistance and, thus, are more likely to tolerate treatment with deltamethrin. CONCLUSIONS: Small changes in susceptibility and low levels of resistance were detected, but the temporal changes of susceptibility should be continuously monitored to adequately guide the actions of vector-control of DC.

  4. Dispersive flight and house invasion by Triatoma guasayana and Triatoma sordida in Argentina

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    Cristina Wisnivesky Colli

    1993-03-01

    Full Text Available Flight activity and invasion of houses by Triatoma sordida and T. guasayana were studied in the Province of Santiago del Estero, Argentina. Spontaneous findings of both species in houses were recorded from 1982 to 1989. Light trap collections were performed in 1982, 1983 and 1984, at the woods surrounding the settlements of Amamá (43 houses and Trinidad (19 houses. Most of the 101 triatomines collected, were unfed and negative for Trypanosoma cruzi. T. guasayana predominated over T. sordida, and both appeared on the lighted screens between 19-31 min (mean 24 after dusk and the catch time was 30-45 min. Although entomological evaluation of 41 houses at Amamá performed in September 1985, just before insecticidal spraying, showed that Triatoma infestans predominated, adults of T. guasayana were collected in sleeping places, in 7 houses (17%. Most triatomines invading houses from then up to 1990 were flying T. guasayana (20/27 and females outnumbered males. Three non infected T. guasayana females were fed on man and two T. guasayana males positive for "T. cruzi like" trypanosomes were unfed. Therefore, visiting hungry adults could transmit T. cruzi to people and introduce wild parasites to the domestic cycle. T. guasayana stands as the main potential substitute of T. infestans in the studied area, and it might play there the same role as T. sordida in Brazil.

  5. Dinâmica da alimentação e dejeção do Triatoma sordida

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    Liléia Diotaiuti

    1995-09-01

    Full Text Available Atualmente, o Triatoma sordida é a espécie de triatomíneo mais capturada no Brasil. Para maior esclarecimento do seu potencial vetorial foi estudada a sua dinâmica de alimentação e dejeções, em comparação com o Triatoma infestans ou outras espécies de reconhecida importância na transmissão do Trypanosoma cruzi. O número de T. sordida e T. infestans alimentados em ratos anestesiados não foi significativamente diferente, nem a média de tempo gasta entre a liberação dos triatomíneos e o início do repasto sanguíneo. A média de tempo para eliminação da primeira dejeção foi significativamente menor em T. infestans que em T. sordida ; no entanto, o número de insetos que defecou durante ou imediatamente após o repasto foi semelhante nas duas espécies. Observações diárias ao longo de um mês indicam que o T. sordida realiza um maior número de picadas por dia do que o T. infestans ou o P. megistus. Estes dados sugerem alguma dificuldade desta espécie em concluir o seu repasto, fato provavelmente importante no seu processo de adaptação ao hospedeiro.

  6. Systematics of Triatoma sordida, T. guasayana and T. patagonica (Hemiptera, Reduviidae

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    David E. Gorla

    1993-09-01

    Full Text Available Because of the relative epidemiological significance of Triatoma sordida, T. guasayana and T. patagonica, and the need to resolve doubts about their taxonomic validity, we report here a detailed taxonomic comparison of the three species using multivariate analysis of morphometric measures combined with comparisons of their genitalia and antennal structures. From the 17 metric variables studied, the length of the second segment of the rostrum and the anteocular length provided a discrimination function able to separate without error T. sordida from T. guasayana and T. patagonica. The multivariate discriminant functions classified T. guasayana and T. patagonica with an error of 2.44%. Comparison of the male genitalia of T. guasayana and T. sordida showed that there are minor differences in the articulatory apparatus, the median process of the pygophore, the phallosome support and the vesica, with bigger differences in the endosomal process and the phallosome. However, the already described male genitalia of T. patagonica is very similar to that of T. sordida. Analysis of antennal structure by scanning electron microscope showed that sensilla distribution around the pedicel is slightly different in the three species and sensilla density is highest in T. sordida and lowest in T. patagonica. The study showed that the three species form a closely related group. The results confirm the earlier classification of sordida and guasayana as separate species, but they raise some doubts about the taxonomic status of T. patagonica.

  7. Comparative Meiotic Studies in Triatoma sordida (Stål and T. guasayana Wygodzinsky & Abalos (Reduviidae, Heteroptera

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    P Rebagliati

    1998-05-01

    Full Text Available Triatoma sordida and T. guasayana are competent Trypanosoma cruzi vectors, with overlapping distribution areas in Argentina. Both species are morphologically similar, and their immature stages are hard to discriminate. Cytogenetic studies in the genus Triatoma reveal scarce karyotypic variations, being 2n= 20 + XY the most frequent diploid number in males. In the present work the meiotic behaviour of different Argentinian populations of T. sordida and T. guasayana has been analyzed; the meiotic karyotype of both species has also been compared. The species differ in total chromosome area and in the relative area of the sex chromosomes. These meiotic karyotypic differences constitute an additional tool for the taxonomic characterization of T. sordida and T. guasayana. The analysis of an interpopulation hybrid of T. sordida (Brazil x Argentina reveals a regular meiotic behaviour, despite the presence of heteromorphic bivalents. Our observations support the hypothesis that karyotype variations through the gain or loss of heterochromatin can not be considered as a primary mechanism of reproductive isolation in Triatoma.

  8. Factors associated with the occurrence of Triatoma sordida (Hemiptera: Reduviidae in rural localities of Central-West Brazil

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    Juliana Chedid Nogared Rossi

    2015-04-01

    Full Text Available This study estimates the factors of artificial environments (houses and peridomestic areas associated with Triatoma sordida occurrence. Manual searches for triatomines were performed in 136 domiciliary units (DUs in two rural localities of Central-West Brazil. For each DU, 32 structural, 23 biotic and 28 management variables were obtained. Multiple logistic regression analysis was performed in order to identify statistically significant variables associated with occurrence of T. sordida in the study areas. A total of 1,057 specimens (99% in peridomiciles, mainly chicken coops of T. sordida were collected from 63 DUs (infestation: 47%; density: ~8 specimens/DU; crowding: ~17 specimens/infested DU; colonisation: 81%. Only six (0.6% out of 945 specimens examined were infected with Trypanosoma cruzi. The final adjusted logistic regression model indicated that the probability of T. sordida occurrence was higher in DU with wooden chicken coops, presence of > 30 animals in wooden corrals, presence of wood piles and presence of food storeroom. The results show the persistence of T. sordida in peridomestic habitats in rural localities of Central-West Brazil. However, the observed low intradomestic colonisation and minimal triatomine infection rates indicate that T. sordida has low potential to sustain high rates of T. cruzi transmission to residents of these localities.

  9. Susceptibility of Triatoma sordida Stal, 1859 (Hemiptera: Reduviidae to alpha-cypermethrin under natural climatic conditions

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    Grasielle Caldas DÁvila Pessoa

    2015-08-01

    Full Text Available Abstract:INTRODUCTION:Despite the recommendations by interpretation of resistance ratios obtained in laboratory bioassays, little is known about the actual impact of these results in the effectiveness of vector control activities in the field. In this context, our objective was to determine the mean value of different resistance ratios obtained by laboratory bioassays performed as part of the chemical control strategies of Triatoma sordida in the field.METHODS:Field bioassays were developed in Monte Azul and Coração de Jesus (Southeast, Brazil. In each location, samples were formed with three domestic units treated with alpha-cypermethrin 20.0% (Alfatek (r 200 SC. One day after spraying, 10 fifth-instar nymphs remained in contact with the surfaces treated (adobe with plaster, adobe without plaster, or wood with insecticide in plastic cones for 72h. Three cones were exposed inside the intradomicile and the peridomicile. The insects in the control group were exposed to an insecticide-free piece of cardboard. Mortality was measured 72h after removal of the insects from the treated surfaces. The tests were realized in triplicate.RESULTS:Mortality was 100.0% in all locations, except for Monte Azul; Landinho (96.6% and Coração de Jesus; Barriguda (96.6%.CONCLUSIONS:Although the resistant populations in laboratory tests proved to be susceptible in the field, this observation is not sufficient to suggest that the cut-off points used to justify the resistance ratio should be changed. In this sense, we recommend that laboratory and field bioassays are carried out with a greater number of Triatominae populations to allow more in-depth consideration of the subject.

  10. Aspectos ecológicos da tripanossomíase americana: XVII - Desenvolvimento da domiciliação triatomínea regional, em centro de endemismo de Triatoma sordida Ecological aspects of South American trypanosomiasis: XVII - The domiciliation development of local triatominae populations in the Triatoma sordida endemic center

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    Oswaldo Paulo Forattini

    1983-06-01

    Full Text Available São apresentados os resultados obtidos mediante as observações sobre o processo de domiciliação triatomínea, após a desinsetização domiciliar, e levadas a efeito no período de janeiro de 1975 a janeiro de 1979. A região estudada inclui-se no domínio paisagístico dos cerrados do Brasil Central, considerado como centro de dispersão de Triatoma sordida. A domiciliação local, inicialmente encontrada, implicou a participação dessa espécie e de Triatoma infestans. Após a realização do expurgo, as pesquisas de vigilância trimestrais e os levantamentos finais evidenciaram a ocorrência de novo processo de domiciliação de triatomíneos, de decurso lento, e resultando em coeficientes de positividade de edifícios acentuadamente inferiores aos registrados por ocasião do levantamento inicial, durante todo o período de observação. Após o expurgo, o valor geral do coeficiente de edifícios (casas e anexos positivos variou do valor mínimo de 1,9 ao máximo de 5,7%, enquanto foi de 40,4% o observado quando do levantamento inicial. Essa situação perdurou pelo tempo mínimo de três anos e meio, o que permite recomendar que a vigilância e a possível aplicação do expurgo seletivo, subseqüentes à desinsetização geral, sejam iniciadas a partir de decorrido tal prazo. Nesse ínterim, recomenda-se a motivação dos habitantes locais, no sentido de obter-lhes a colaboração para a denúncia de novos focos intradomiciliares. Na transferência da infecção tripanossômica para o ambiente domiciliar evidenciou-se papel importante desempenhado por vertebrados domiciliados ou em domiciliação, em especial modo ratos (Rattus e gambás (Didelphis, de hábitos ubiquistas. No processo de evolução da nova domiciliação triatomínea, coube papel preponderante a T. sordida mediante a ocupação inicial do peridomicílio. O reaparecimento de T. infestans deveu-se principalmente à influência da atividade humana, em especial modo, a

  11. Estadísticos poblacionales de Triatoma sordida Stäl 1859 (Hemiptera : Reduviidae en condiciones experimentales Population statistics of Triatoma sordida Stäl 1859 (Hemiptera: Reduviidae in experimental conditions

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    Elena B. Oscherov

    1996-10-01

    Full Text Available Se realizó el seguimiento longitudinal de cuatro cohortes de 100 huevos cada una. Los insectos fueron criados a 28°C ± 3°C y 63 % ± 10% de humedad relativa, y alimentados cada siete días sobre gallina (Gallus domesticus, durante 40 min. Las cohortes fueron controladas una vez por semana. Se observó un valor constante de supervivencia en las cuatro cohortes, sin picos importantes. La expectativa de vida de los machos, en promedio, fue de 37,4 semanas y la de las hembras 36,8. El tiempo generacional demandó 61,7 semanas. La tasa reproductiva neta indica que esta población se incrementará 143,3 veces con cada generación. La tasa intrínseca de crecimiento natural fue de 0,082 por semana. El máximo valor reproductivo se verificó, en promedio, a la tercer semana del ingreso al estado adulto. La distribución estable de edades de T. sordida sería de 32% huevos, 26% ninfas de primer estadio, 19% ninfas de segundo estadio, 13% ninfas de tercer estadio, 6% ninfas de cuarto estadio, 3% ninfas de quinto estadio y 1% de adultos. Es la primera cita para Argentina sobre la tabla de vida de T. sordida. El análisis de estos parámetros permite considerar que esta especie se comporta como "K estratega", en condiciones experimentales.Foram estudados diferentes parâmetros populacionais de Triatoma sordida em condições de laboratório. Realizou-se um seguimento longitudinal de quatro coortes de 100 ovos cada uma; os insetos foram criados a 28°C ± 3°C e 63% ± 10% de umidade relativa, e alimentados cada sete dias sobre galinha (Gallus domesticus durante 40'. As coortes foram controladas uma vez por semana. A expectativa de vida média dos machos foi de 37,4 semanas e a das fêmeas de 36,8.O tempo médio de desenvolvimento foi de 61,7 semanas. A taxa reprodutiva líquida indica que esta população aumentará 143,2 vezes durante cada geração. A taxa intrínseca do crecimento natural foi de 0,082 por semana. A média do valor máximo reprodutivo

  12. The ecology of Triatoma sordida in natural environments in two different regions of the state of Minas Gerais, Brazil Ecologia do Triatoma sordida no ambiente silvestre de duas diferentes regiões do estado de Minas Gerais, Brasil

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    L. Diotaiuti

    1993-06-01

    em comparação com o Triângulo, o que pode explicar a maior taxa de infecção dos triatomíneos no norte. Maior atenção ao processo de domiciliação do T. sordida no norte de Minas Gerais é recomendada, onde, nos últimos anos, tem-se observado urna profunda intervenção do homem sobre o ambiente natural, paralelamente ao aumento da população deste triatomíneo nos peridomicílios.

  13. Biology of Triatominae (Reduviidae Hemiptera from North of Formosa County (Goiás-Brazil I. Length of life cycle of Triatoma sordida (Stal. 1859

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    Dalva A. Mello

    1976-12-01

    Full Text Available In the present paper the life cycle of Triatoma sordida was studied. The mean length from egg to adult was 213 days. The mean length in days from each stage was: 24.3 (± 1.30 for the first. 32.8 (± 1.45 (2nd, 36.1 (± 1.50 (3rd, 44.6 (± 1.85 (4th and 52.0 (± 1.92 (5th. The mean egg incubation períod was 23.2 (± 1.40. Overall mortality was 18.8% and egg viability was 82.5%.Foi estudado no presente trabalho o ciclo biológico do Triatoma sordida A média da duração do ciclo de ovo a adulto foi 213 dias. A média de duração em dias para cada estágio foi 24,3 (± 1,30 para o primeiro, 32,8 (± 1,45 para o segundo, 36,1 (± 1,50 para o terceiro, 44,6 (± 1,85 para o quarto e 52,0 (± 1,92 para o quinto. A média do período de incubação dos ovos foi 23,2 (± 1,40. A mortalidade em todos ps estágios foi 18,8% e a viabilidade dos ovos foi 82,5%.

  14. Aspectos ecológicos da Tripanossomíase americana: VI - Persistência do Triatoma sordida apósalteração ambiental e suas possíveis relações com a dispersão da espécie Ecological aspects of South American trypanosomiasis: VI. Permanence of Triatoma sordida after environmental alteration and its possibles relation to dispersion

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    Oswaldo Paulo Forattini

    1974-09-01

    Full Text Available São relatadas as observações sobre a persistência do Triatoma sordida em área onde foi procedida alteração temporária da cobertura vegetal. Os resultados obtidos demonstram a capacidade de resistência do triatomíneo a esse fator. Procurou-se relacionar esse aspecto à distribuição da espécie no Estado de São Paulo, Brasil. Existe certa semelhança entre os dados disponíveis a esse respeito e a evolução da atividade agrícola na região. Finalmente são considerados também os aspectos climáticos que podem influir na dispersão da espécie.Studies about permanence of Triatoma sordida after temporary alteration of the local vegetation are related. Results reached showed a bug resistence to the devastation of shrubby vegetation by fire. An attempt is made for to relate this particular aspect with species distribution in the S. Paulo State, Brazil. There are some analogies between the available data about this and the development of agrarian activities. Beside the climatic one, this factor may be considered as favorable to the Triatoma sordida dispersion, by the natural environmental alteration who if follows.

  15. Presencia de Triatoma sordida Stäl, 1859 en ecotopos urbanos de la ciudad de Corrientes, Argentina

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    María E. Bar

    1993-04-01

    Full Text Available Con el objeto de verificar la presencia de T. sordida Stäl 1859, en ecotopos urbanos de la ciudad de Corrientes, Argentina se tomaron como base un habitat extradomiciliario y la vivienda humana. En el palomar situado en el Parque Mitre fue realizado un censo completo de población de T. sordida para lo cual fueron disecados 400 nidos de paloma (Columbia livia. Los ejemplares de triatominos detectados en las viviendas fueron enviados por los pobladores al Laboratorio de Artrópodos. Los triatominos fueron determinados sistemáticamente y por clase de edad. La materia fecal de los mismos fue examinada al microscopio para la identificación del Trypanosoma cruzi. En el palomar se colectaron: 6 huevos, 45 ninfas de primer estadío, 110 ninfas de segundo estadío, 226 ninfas de tercer estadío, 303 ninfas de cuarto estadío, 1.103 ninfas de quinto estadío, 12 hembras y 11 machos. Se constató la presencia de esta especie en 27 viviendas localizadas en 8 barrios de la Ciudad. Se capturaron 13 huevos, 33 ninfas de diferentes estadíos, 27 hembras y 9 machos, totalizando 82 individuos. Ningún triatomino resultó infectado por T. cruzi. Los adultos de T. sordida se desplazaron hacia el domicilio en primavera y verano. Es de destacar que el 33% del material hallado en las viviendas correspondió a hembras. Se ha comprobado la adaptación de T. sordida a un habitat extradomiciliario y su tendencia a invadir la vivienda humana, hecho que se debería ser tenido en cuenta en los programas de control de vectores.

  16. Pathogenicity of Evlachovaea sp (Hyphomycetes, a new species isolated from Triatoma sordida, in Chagas' disease vectors Patogenicidade de Evlachovaea sp (Hyphomycetes, uma nova espécie isolada de Triatoma sordida, para vetores da doença de Chagas

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    Christian Luz

    2004-03-01

    Full Text Available Evlachovaea sp was tested on nymphs of 5 Triatoma spp 5 Rhodnius spp, Panstrongylus herreri and Dipetalogaster maximus at 25°C, 75% humidity and humidity >98%. Most species showed susceptibility to fungal infection at high humidity. Mortality was reduced at 75% humidity. Fungal development was observed on 69.5% of cadavers.Evlachovaea sp foi testada em ninfas de 5 Triatoma spp, 5 Rhodnius spp, Panstrongylus herreri e Dipetalogaster maximus a 25°C, 75% umidade e umidade >98%. A maioria das espécies foi suscetível à infecção em umidade alta. Mortalidade foi reduzida a 75% de umidade. O fungo desenvolveu em 69.5% dos cadáveres.

  17. Estadísticos poblacionales de Triatoma sordida Stäl 1859 (Hemiptera : Reduviidae en condiciones experimentales

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    Oscherov Elena B.

    1996-01-01

    Full Text Available Se realizó el seguimiento longitudinal de cuatro cohortes de 100 huevos cada una. Los insectos fueron criados a 28°C ± 3°C y 63 % ± 10% de humedad relativa, y alimentados cada siete días sobre gallina (Gallus domesticus, durante 40 min. Las cohortes fueron controladas una vez por semana. Se observó un valor constante de supervivencia en las cuatro cohortes, sin picos importantes. La expectativa de vida de los machos, en promedio, fue de 37,4 semanas y la de las hembras 36,8. El tiempo generacional demandó 61,7 semanas. La tasa reproductiva neta indica que esta población se incrementará 143,3 veces con cada generación. La tasa intrínseca de crecimiento natural fue de 0,082 por semana. El máximo valor reproductivo se verificó, en promedio, a la tercer semana del ingreso al estado adulto. La distribución estable de edades de T. sordida sería de 32% huevos, 26% ninfas de primer estadio, 19% ninfas de segundo estadio, 13% ninfas de tercer estadio, 6% ninfas de cuarto estadio, 3% ninfas de quinto estadio y 1% de adultos. Es la primera cita para Argentina sobre la tabla de vida de T. sordida. El análisis de estos parámetros permite considerar que esta especie se comporta como "K estratega", en condiciones experimentales.

  18. Cutaneous allergic reactions to Triatoma infestans after xenodiagnosis Reações cutâneas alérgicas ao Triatoma infestans após Xenodiagnóstico

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    Kenneth E. Mott

    1980-12-01

    Full Text Available We determined the frequency of cutaneous allergic reactions to bites of Triatoma infestans during xenodiagnosis in a rural community where Panstrongylus megistrus is the only domestic vector of Trypanosoma cruzi. Localized urticaria or more intense cutaneous allergic reactions at 48 and 72 hours were observed in 86.7% and 82.1% respectively of the individuals in our study. Urticaria was more severe in children and older adults and in women than in men. The high frequency of reactions suggests either cutaneous reactivity to T. infestans without prior sensitization or cross reactivity between P. megistus and T. infestans. A single application of topical corticosteroid or antihistamine medication did not reduce the cutaneous reactions.A freqüência de reações cutâneas alérgicas ás picadas de Triatoma infestans após xenodiagnóstico foi determinada numa comunidade rural na qual Panstrongylus megistus é o único vetor doméstico. Urticária focal ou reações cutâneas alérgicas mais intensas foram observadas em 86.7% e em 82.1% dos participantes estudados, respectivamente ás 48 e ás 72 horas. A urticária foi mais severa em crianças e adultos idosos e mais severa em mulheres do que em homens. A alta freqüência das reações sugere a existência de reatividade cutânea ao T. infestans, sem prévia sensibilização, ou uma reatividade cruzada entre P. megistus e T. infestans. A aplicação tópica de pomada de corticosteróide ou de anti-histamínico não reduziu as reações cutâneas alérgicas.

  19. Estudos sobre a resistência ao jejum e aspectos nutricionais de Triatoma lecticularia (Stal, 1859 (Hemiptera, Reduviidae, Triatominae Studies on the resistance to fasting and nutritional aspects of Triatoma lecticularia (Stal, 1859 (Hemiptera, Reduviidae, Triatominae

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    José Jurberg

    1989-09-01

    Full Text Available Foi feito um estudo sobre a resistência ao jejum em todas as fases evolutivas e pesagens em diferentes situações nutricionais de Triatoma lecticularia (alimentado; não alimentado; na morte após o jejum com temperatura e umidade registradas. Observou-se que os períodos de resistência das fases ninfais apresentaram médias (dias crescentes: 1º: 45,84; 2º: 61; 3º: 88,74; 4º: 123,47; 5º 162,30. Na fase adulta as médias foram aproximadas à do 3º estádio (para os machos 88,94 e para as fêmeas 83,66. O procedimento de pesagens permitiu registrar a quantidade de sangue ingerido, a perda de peso durante o jejum e o respectivo percentual em relação ao peso inicial. Esta espécie tem assinalada sua distribuição geográfica na região Neártica, onde tem sido encontrda infectada com Trypanosoma cruzi associada a Neotoma micropus Baird e Spermophilus variagatus (Erxeleben.The resistance to fasting of Triatoma lecticularia was studied in all phases of the life cycle and the insect weighed in different nutritional situations (fed, not fed, death after starvation. The temperature and humidity level were also recorded. The nymphal phases showed increasing resistance to fasting as demonstrated by the following averages (days 1st 45.84; 2nd 61; 3rd 88.74; 4th 123.44; 5th 162.30. Upon the adult phase, the averages were similar to those of the 3rd stage for the male insects 88.94 and for females 83.66. The weighing technique allowed for the measeurement of the quantity of blood ingested, the weight loss during the fast and the percentage weight lost as related to the initial weight. The species is found in the Neartic region where it has been found infected with Trypanosoma cruzi and is associated with the terrestial rodents Neotoma micropus Baird and Spermophilus variagatus (Erxelebem.

  20. Selective Insecticide Applications Directed Against Triatoma infestans (Hemiptera: Reduviidae) Affected a Nontarget Secondary Vector of Chagas Disease, Triatoma garciabesi.

    Science.gov (United States)

    Rodríguez-Planes, L I; Vazquez-Prokopec, G M; Cecere, M C; Canale, D M; Gürtler, R E

    2016-01-01

    The control of nondomiciliated triatomine species adapted to peridomestic habitats represents a challenge because they are connected to sylvatic colonies, and pyrethroid insecticides have limited effects outdoors. The effects of residual insecticide spraying have rarely been assessed on secondary triatomines. Triatoma garciabesi (Carcavallo, Martinez, Cichero, Prosen & Ronderos, 1967) is a nontarget vector that inhabits the dry western Chaco region, and a member of the Triatoma sordida Stål 1859 complex. Little is known on the capacity of T. garciabesi to invade and establish viable domestic or peridomestic colonies, and on its response to residual insecticide sprays directed against Triatoma infestans Klug 1834. The presence and abundance of triatomines were assessed by timed manual collections annually or biannually (spring and fall) during 10 yr after a community-wide insecticide spraying campaign and selective insecticide sprays directed against T. infestans in a rural village of northwestern Argentina. T. garciabesi mainly occupied peridomestic habitats associated with chickens, and was unable to colonize human sleeping quarters. Trees with chickens occurred in nearly all houses and were infested in >25% of the occasions. The abundance of bugs at house-compound level was best explained by a generalized estimating equation model that included selective insecticide sprays during the previous semester (negative effects), chicken abundance (positive effects), seasonality, and their interactions. Our results suggest that insecticide applications targeting T. infestans affected the abundance of T. garciabesi, and reduced the likelihood of future infestation.

  1. On Pomacea sordida (Swainson, 1823 (Prosobranchia, Ampullariidae Sobre Pomacea sordida (Swaison, 1823 (Prosobranchia, Ampullariidae

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    Silvana Carvalho Thiengo

    1989-09-01

    Full Text Available A description of Pomacea sordida (Swainson, 1823 collected in Caxias and Nova Iguaçu, state of Rio de Janeiro, is presented. The shell is globose, heavy, whith greenish or horn-colored periostracum and dark spinal bands; apex subelevated, 4-5 moderately shoudered whorls, increasing rather rapidly and separated by deep suture. Aperture large, moderately round, yellowish or violaceous; lip thick and sometimes dark brown; umbilicus large and deep; operculum corneous and heavy, entirely closing the aperture. Ratios: shell width/shell length = 0.81-0.91 (mean 0.86; aperture length/shell length = 0.66-0.75 (mean 0.70. Testis, spermiduct and penis pouch as in Pomacea lineata (Spix, 1827. Seminal vesicle whitish and bean-shaped. Prostate cylindric and narrow, cream in coloar as the testis. Penis whiplike whith a closed circular spermiduct. Penial sheath elongated and tapered, with its distal tip turned to the right; outer basal gland situated on the left; inner median gland rounded; apical gland elongated and wrinkled. Ovary composed of branched whitish tubules lying superficially on the digestive gland; oviduct and seminal receptacle as in P. lineata; albumen gland yellowish - orange. Vestigial male copulatory apparatus (penis and its sheath present in all females examined.Nesse trabalho é apresentada a descrição de Pomacea sordida (Swainson, 1823, coletada em Caxias e Nova Iguaçu, Estado do Rio de Janeiro. Concha globosa, espessa, com perióstraco esverdeado ou castanho e com faixas espirais escuras; ápice pouco elevado, 4-5 giros moderadamente arredondados, crescendo relativamente rápido, separados por suturas profundas. Abertura grande, moderadamente arredondada, amarelada ou violácea; lábio espesso e algumas vezes marrom escuro; umbílico grande e profundo; opérculo córneo e espesso, fechando completamente a abertura. Razões: largura da concha/comprimento da concha=0.81-0.91 (média 0.86; comprimento da abertura/comprimento da concha=0

  2. The peri-urban interface and house infestation with Triatoma infestans in the Argentine Chaco: an underreported process?

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    Yael M Provecho

    2014-11-01

    Full Text Available Peri-urban infestations with triatomine bugs, their sources and their dynamics have rarely been investigated. Here, we corroborated the reported occurrence of Triatoma infestans in a peri-urban area and in neighbouring rural houses in Pampa del Indio, in the Argentine Chaco, and identified its putative sources using spatial analysis and demographic questionnaires. Peri-urban householders reported that 10% of their premises had triatomines, whereas T. infestans was collected by timed manual searches or community-based surveillance in only nine (3% houses. Trypanosoma cruzi-infected T. infestans and Triatoma sordida were collected indoors only in peri-urban houses and were infected with TcV and TcI, respectively. The triatomines fed on chickens, cats and humans. Peri-urban infestations were most frequent in a squatter settlement and particularly within the recently built mud houses of rural immigrants, with large-sized households, more dogs and cats and more crowding. Several of the observed infestations were most likely associated with passive bug transport from other sources and with active bug dispersal from neighbouring foci. Thus, the households in the squatter settlement were at a greater risk of bug invasion and colonisation. In sum, the incipient process of domestic colonisation and transmission, along with persistent rural-to-urban migratory flows and unplanned urbanisation, indicate the need for active vector surveillance and control actions at the peri-urban interface of the Gran Chaco.

  3. Sobre a variação intra-específica em Triatoma dimidiata (Latreille e Triatoma infestans (Klug (Hemiptera, Reduviidae

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    Herman Lent

    1985-09-01

    Full Text Available A observação de oito exemplares de Triatoma dimidiata (Latreille, 1811 de procedência diversas, com vistas a procurar caracterizar as estruturas da genitália externa dos machos (processo do endosoma, suporte do falosoma, falosoma e visica levou-nos a constatar a mesma variabilidade que a espécie demonstra em relação aos seus caracteres externos de colorido e relações da cabeça e do rosto. Por associação de idéias, ampliamos um estudo anterior sobre a genitália masculina de Triatoma infestans (Klug, 1834, também espécie de larga distribuição geográfica, porém com caracteres externos estáveis. Encontramos assim mesmo, embora em percentagem pequena, que os espinhos do processo do endosoma, habitualmente em número reduzido, podiam não existir. Desta espécie observamos quinze exemplares, de procedências diversas.The analysis of eight speciemens of Triatoma dimidiata (Latreille, 1811 of diverse origins, in order to characterize the structure of the male external genitalia (endosoma process, struts, phallosoma, vesica led us to point out that the same variability is demonstrate by the species in relation to its external colour characters and the relation between the head and rostrum. Triatoma dimidiata is a vector of Chagas' disease in several American countries, from Mexico (extreme north of its distribution to the north of South America, including Colombia, Venezuela, Ecuador and Peru. Similarly we have carried out a large scale study on male genitalia of Triatoma infestans (Klug, 1834 which also has a wide geographic distribution, but with stable external characters. The spines of the endosoma process which generally are few in number, might not exist in a small percentage of cases. We analysed fifteen specimens of diverse origins.

  4. Arreglo de los polígonos del exocorion de huevos eclosionados de algunas especies de los géneros Triatoma Laporte, Meccus Stal y Nesotriatoma Usinger (Heteroptera: Reduviidae.

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    Glauco López

    2009-12-01

    Full Text Available Resumen. En este trabajo se estudiaron estructural y cuantitativamente las celdas exocoriales de huevos eclosionados de Triatoma infestans (Klug, Triatoma lenti Sherlock & Serafim, Triatoma maculata (Erichson, Triatoma sordida (Stal, Meccus picturatus (Usinger, Meccus longipennis (Usinger, Meccus pallidipennis (Stal and Nesotriatoma flavida (Neiva. Los huevos se analizaron por regiones: caudal, media y cefálica. Se encontró que el hexágono fue el polígono más abundante en todas las regiones del huevo y en todas las especies, seguido del pentágono y del heptágono; se encontraron diferencias estadísticamente significativas en el número tanto de hexágonos y pentágonos entre regiones de una misma especie y entre la misma región de las diferentes especies, sin embargo, el número promedio de heptágonos no varió al comparar la región media entre las diferentes especies y al comparar las diferentes regiones en una misma especie. No se encontraron diferencias estadísticamente significativas en el promedio de polígonos, segmentos totales, heptágonos, hexágonos y pentágonos, al comparar las especies examinadas del género Meccus. Se propone un protocolo de identificación y análisis de teselaciones, con el cual se encontró que en 108 campos examinados de 385.000 µm2 cada uno, se identificaron teselaciones compartidas en los 108 campos observados con determinadas combinaciones de heptágonos, hexágonos y pentágonos, y se observaron teselaciones no compartidas en la totalidad de los campos, con combinaciones de polígonos de cuatro a nueve lados, y cada teselación se representó por un grafo. Se discuten las implicaciones taxonómicas, funcionales, filogenéticas y construccionales de los arreglos poligonales descritos.Arragement of exochorial polygons of hatched eggs in several species of the genera Triatoma Laporte, Meccus Stal y Nesotriatoma Usinger (Heteroptera: Reduviidae. Abstract. In this study a structural and quantitative

  5. TREATMENT OF A PENTACHLOROPHENOL AND CREOSOTE-CONTAMINATED SOIL USING THE LIGNIN-DEGRADING FUNGUS PHANERO- CHAETE SORDIDA: A FIELD DEMONSTRATION

    Science.gov (United States)

    The feasibility of large-scale fungal bioaugmentation was evaluated by assessing the ability of the lignin-degrading fungus Phanerochaete sordida to decrease the soil concentrations of pentachlorophenol (PCP) and 13 priority pollutant polynuclear aromatic (PNA) creosote component...

  6. Decolorization of mixtures of different reactive textile dyes by the white-rot basidiomycete Phanerochaete sordida and inhibitory effect of polyvinyl alcohol.

    Science.gov (United States)

    Harazono, Koichi; Nakamura, Kazunori

    2005-03-01

    We tried to decolorize mixtures of four reactive textile dyes, including azo and anthraquinone dyes, by a white-rot basidiomycete Phanerochaete sordida. P. sordida decolorized dye mixtures (200 mg l-1 each) by 90% within 48 h in nitrogen-limited glucose-ammonium media. Decolorization of dye mixtures needed Mn2+ and Tween 80 in the media. Manganese peroxidase (MnP) played a major role in dye decolorization by P. sordida. Decolorization of dye mixtures by P. sordida was partially inhibited by polyvinyl alcohol (PVA) that wastewaters from textile industries often contain. This was caused by an inhibitory effect of PVA on the decolorization of Reactive Red 120 (RR120) with MnP reaction system. Second addition of Tween 80 to the reaction mixtures in the presence of PVA improved the decolorization of RR120. These results suggest that PVA could interfere with lipid peroxidation or subsequent attack to the dye.

  7. The biology of three Mexican-American species of Triatominae (Hemiptera: Reduviidae: Triatoma recurva, Triatoma protracta and Triatoma rubida

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    José Alejandro Martínez-Ibarra

    2012-08-01

    Full Text Available The values of biological parameters related to hatching, lifespan, the number of blood meals between moults, mortality, time lapse before the beginning of feeding, feeding time and defecation delay for each instar of three Mexican-American species of Triatominae, Triatoma recurva, Triatoma protracta (former subspecies protracta and Triatoma rubida (former subspecies uhleri, were evaluated and compared. No significant (p > 0.05 differences were recorded among the three species with respect to the average time required to hatch. This time was approximately 19 days. The average egg-to-adult development time was significantly (p < 0.05 shorter for T. rubida. The number of blood meals at each nymphal instar varied from one-five for each species. The mortality rates were higher for the first-instar nymphs of the three species studied. The mean time lapse before the beginning of feeding was between 0.3-3 min for most nymphs of all instars of each species studied. The mean feeding time was the longest for T. recurva, followed by T. protracta. The defecation delay was less than 10 min for T. recurva and T. rubida. Given these results, only T. rubida should be considered an important potential vector of Trypanosoma cruzi transmission to humans in areas of Mexico where these species exist, whereas T. recurva and T. protracta would be of secondary importance.

  8. Comparative study of the stridulatorium sulcus, buccula and rostrum of nymphs of Triatoma klugi Carcavallo et al, Triatoma vandae Carcavallo et al and Triatoma williami Galvao et al (Hemiptera: Redivide)

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Maria B.A.; Jurberg, Jose; Galvao, Cleber; Barbosa, Helene S. [Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ (Brazil). Lab. de Biologia Estrutural. Lab. Nacional e Internacional de Referencia em Taxonomia de Triatomineos

    2010-01-15

    Ultrastructural analysis of the ventral region of the head - rostrum, buccula and stridulatorium sulcus - of 1st, 3rd and 5th instars of Triatoma klugi Carcavallo et al, Triatoma vandae Carcavallo et al, and Triatoma williami Galvao et al, are described in here. Morphological differences in the analyzed structures for all three Triatoma species studied were detected under scanning electron microscopy, allowing their grouping by their morphological similarities. Species-specific differences at each nymphal development stage were analyzed as well. (author)

  9. Contribución al conocimiento de Salvia sordida. Benth. Historia natural y conservación

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    Fernández Alonso José Luis

    2004-12-01

    Full Text Available Salvia sordida es una especie de labiada endémica de Colombia, que está constituida por arbustos que pueden llegar hasta los 4 m de altura, con indumentos densos en el envés de las hojas, en los ejes de la inflorescencia y en los cálices, con corolas moradas de 15 mm en promedio; presenta una variabilidad morfológica que se manifiesta en su hábito, en la forma, coloración e indumento de sus hojas y en la densidad de su inflorescencia, producida posiblemente como adaptación a diferentes condiciones de luz. Existen tres poblaciones de esta especie que indican que S. sordida se distribuye en el altiplano cundiboyacense en un rango altitudinal entre los 2.600 y los 2.800 msnm. De acuerdo con observaciones hechas en la población tipo, ubicada en la vereda Torca en jurisdicción de Bogotá, el hábitat de la salvia se caracteriza por presentar
    un clima con una temperatura promedio de 13º C, una humedad relativa media de 74% y una precipitación de 1.200 mm anuales; en áreas de piedemonte, con suelos originados de cenizas volcánicas poco evolucionados, con textura franco-arenosa en la superficie, muy fuertemente ácidos y con una baja fertilidad; el hábitat de S. sordida hace parte del bosque de Cordia-Oreopanax, se caracteriza por un estrato arbustivo predominante y un estrato herbáceo bien representado, compuesto por 101 especies tanto del bosque climácico como de ambientes alterados o cultivadas, lo que indicaría que la comunidad de S. sordida hace parte de las etapas de sucesión secundaria del bosque; por lo tanto el hábitat se caracteriza por ser inestable y está delimitado por las condiciones de alteración de la vegetación y la variación de los factores de humedad y luz en un rango altitudinal estrecho. Salvia sordida presenta una floración y fructificación constantes y en ella se han reconocido 14 visitantes florales tanto en campo como en cultivo, que incluyen especies de Diptera, Hymenoptera, Lepidoptera y Aves. De

  10. Tratamentos focais e totais com inseticidas de ação residual para o controle de Triatoma brasiliensis e Triatoma pseudomaculata no Nordeste brasileiro

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    Oliveira Filho Alfredo M.

    2000-01-01

    Full Text Available Para controle eficiente dos triatomíneos Triatoma brasiliensis e Triatoma pseudomaculata, foi feito um ensaio de campo em Boa Viagem, Ceará, de modo a comparar a borrifação convencional versus tratamento focal com deltametrina 5% SC, dose 25 mg i.a./m² e o organofosforado malation lenta liberação 8.3% SR, dose 2g i.a./m². O ensaio incluiu aleatoriamente 1.541 casas, separadas em quatro grupos. Em dois deles foi aplicada borrifação focal ­ tratamento PT com deltametrina dentro das casas e no peridomicílio e PL que recebeu malation lenta liberação nas mesmas circunstâncias. Os outros dois tiveram tratamento convencional, isto é, aplicação total ­ PT com deltametrina no intra e peridomicílio e PL, tratado com deltametrina dentro das casas e malation lenta liberação no peridomicílio. As avaliações entomológicas aos 6 e 12 meses pós-tratamentos mostraram melhor resultado para o tratamento misto, grupo PL, provavelmente em decorrência da boa performance do piretróide dentro das casas e da formulação de lenta liberação nas condições hostis do peridomicílio. Os abrigos dos animais domésticos sofreram modificações ao longo do ano, colaborando com a redução da performance dos inseticidas no peridomicílio.

  11. Impregnated netting slows infestation by Triatoma infestans.

    Science.gov (United States)

    Levy, Michael Z; Quíspe-Machaca, Victor R; Ylla-Velasquez, Jose L; Waller, Lance A; Richards, Jean M; Rath, Bruno; Borrini-Mayori, Katty; del Carpio, Juan G Cornejo; Cordova-Benzaquen, Eleazar; McKenzie, F Ellis; Wirtz, Robert A; Maguire, James H; Gilman, Robert H; Bern, Caryn

    2008-10-01

    We used sentinel animal enclosures to measure the rate of infestation by the Chagas disease vector, Triatoma infestans, in an urban community of Arequipa, Peru, and to evaluate the effect of deltamethrin-impregnated netting on that rate. Impregnated netting decreased the rate of infestation of sentinel enclosures (rate ratio, 0.23; 95% confidence interval, 0.13-0.38; P < 0.001), controlling for the density of surrounding vector populations and the distance of these to the sentinel enclosures. Most migrant insects were early-stage nymphs, which are less likely to carry the parasitic agent of Chagas disease, Trypanosoma cruzi. Spread of the vector in the city therefore likely precedes spread of the parasite. Netting was particularly effective against adult insects and late-stage nymphs; taking into account population structure, netting decreased the reproductive value of migrant populations from 443.6 to 40.5. Impregnated netting can slow the spread of T. infestans and is a potentially valuable tool in the control of Chagas disease.

  12. Immunogenic salivary proteins of Triatoma infestans: development of a recombinant antigen for the detection of low-level infestation of triatomines.

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    Alexandra Schwarz

    Full Text Available BACKGROUND: Triatomines are vectors of Trypanosoma cruzi, the etiological agent of Chagas disease in Latin America. The most effective vector, Triatoma infestans, has been controlled successfully in much of Latin America using insecticide spraying. Though rarely undertaken, surveillance programs are necessary in order to identify new infestations and estimate the intensity of triatomine bug infestations in domestic and peridomestic habitats. Since hosts exposed to triatomines develop immune responses to salivary antigens, these responses can be evaluated for their usefulness as epidemiological markers to detect infestations of T. infestans. METHODOLOGY/PRINCIPAL FINDINGS: T. infestans salivary proteins were separated by 2D-gel electrophoresis and tested for their immunogenicity by Western blotting using sera from chickens and guinea pigs experimentally exposed to T. infestans. From five highly immunogenic protein spots, eight salivary proteins were identified by nano liquid chromatography-electrospray ionization-tandem mass spectrometry (nanoLC-ESI-MS/MS and comparison to the protein sequences of the National Center for Biotechnology Information (NCBI database and expressed sequence tags of a unidirectionally cloned salivary gland cDNA library from T. infestans combined with the NCBI yeast protein sub-database. The 14.6 kDa salivary protein [gi|149689094] was produced as recombinant protein (rTiSP14.6 in a mammalian cell expression system and recognized by all animal sera. The specificity of rTiSP14.6 was confirmed by the lack of reactivity to anti-mosquito and anti-sand fly saliva antibodies. However, rTiSP14.6 was recognized by sera from chickens exposed to four other triatomine species, Triatoma brasiliensis, T. sordida, Rhodnius prolixus, and Panstrongylus megistus and by sera of chickens from an endemic area of T. infestans and Chagas disease in Bolivia. CONCLUSIONS/SIGNIFICANCE: The recombinant rTiSP14.6 is a suitable and promising

  13. Biological activity of Schinus molle on Triatoma infestans.

    Science.gov (United States)

    Ferrero, A A; Werdin González, J O; Sánchez Chopa, C

    2006-07-01

    Hexanic extracts from leaves and fruits of Schinus molle were tested for repellent and insecticidal properties against first instar nymphs and eggs of Triatoma infestans, the vector of Chagas' disease. Leaf and fruit extracts were highly repellent for first nymphs. Fruit extracts had also ovicidal activity.

  14. Tratamentos focais e totais com inseticidas de ação residual para o controle de Triatoma brasiliensis e Triatoma pseudomaculata no Nordeste brasileiro Focal and total residual insecticide spraying to control Triatoma brasiliensis and Triatoma pseudomaculata in Northeast Brazil

    Directory of Open Access Journals (Sweden)

    Alfredo M. Oliveira Filho

    2000-01-01

    Full Text Available Para controle eficiente dos triatomíneos Triatoma brasiliensis e Triatoma pseudomaculata, foi feito um ensaio de campo em Boa Viagem, Ceará, de modo a comparar a borrifação convencional versus tratamento focal com deltametrina 5% SC, dose 25 mg i.a./m² e o organofosforado malation lenta liberação 8.3% SR, dose 2g i.a./m². O ensaio incluiu aleatoriamente 1.541 casas, separadas em quatro grupos. Em dois deles foi aplicada borrifação focal ­ tratamento PT com deltametrina dentro das casas e no peridomicílio e PL que recebeu malation lenta liberação nas mesmas circunstâncias. Os outros dois tiveram tratamento convencional, isto é, aplicação total ­ PT com deltametrina no intra e peridomicílio e PL, tratado com deltametrina dentro das casas e malation lenta liberação no peridomicílio. As avaliações entomológicas aos 6 e 12 meses pós-tratamentos mostraram melhor resultado para o tratamento misto, grupo PL, provavelmente em decorrência da boa performance do piretróide dentro das casas e da formulação de lenta liberação nas condições hostis do peridomicílio. Os abrigos dos animais domésticos sofreram modificações ao longo do ano, colaborando com a redução da performance dos inseticidas no peridomicílio.To efficiently control the triatomines Triatoma brasiliensis and Triatoma pseudomaculata, a field trial was performed to compare conventional versus focal spraying of deltamethrin 5% SC at 25 mg a.i./m² and the slow-release organophosphate malathion 8.3% SR at 2g a.i./m². The assay took place in the county of Boa Viagem, Ceará State, with 1541 households, randomly separated into 4 groups. Two of them received focal spraying: PT, treated with deltamethrin indoors and in the peridomicile, and PL, which received slow-release malathion in the same circumstances. The other groups received conventional, i.e., total application: PT with deltamethrin in the intra- and peridomicile, and PL, which was treated with

  15. Structure of the Triatoma virus capsid

    Energy Technology Data Exchange (ETDEWEB)

    Squires, Gaëlle; Pous, Joan [Laboratoire de Virologie Moléculaire et Structurale, CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette CEDEX (France); Agirre, Jon [Fundación Biofísica Bizkaia, Barrio Sarriena S/N, 48940 Leioa, Bizkaia (FBB) (Spain); Unidad de Biofísica (UBF, CSIC, UPV/EHU), PO Box 644, 48080 Bilbao (Spain); Rozas-Dennis, Gabriela S. [U.N.S., San Juan 670 (8000) Bahía Blanca (Argentina); U.N.S., Avenida Alem 1253 (8000) Bahía Blanca (Argentina); Costabel, Marcelo D. [U.N.S., Avenida Alem 1253 (8000) Bahía Blanca (Argentina); Marti, Gerardo A. [Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT, La Plata, CONICET-UNLP), Calle 2 No. 584 (1900) La Plata (Argentina); Navaza, Jorge; Bressanelli, Stéphane [Laboratoire de Virologie Moléculaire et Structurale, CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette CEDEX (France); Guérin, Diego M. A., E-mail: diego.guerin@ehu.es [Fundación Biofísica Bizkaia, Barrio Sarriena S/N, 48940 Leioa, Bizkaia (FBB) (Spain); Unidad de Biofísica (UBF, CSIC, UPV/EHU), PO Box 644, 48080 Bilbao (Spain); Rey, Felix A., E-mail: diego.guerin@ehu.es [Laboratoire de Virologie Moléculaire et Structurale, CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette CEDEX (France)

    2013-06-01

    The crystallographic structure of TrV shows specific morphological and functional features that clearly distinguish it from the type species of the Cripavirus genus, CrPV. The members of the Dicistroviridae family are non-enveloped positive-sense single-stranded RNA (+ssRNA) viruses pathogenic to beneficial arthropods as well as insect pests of medical importance. Triatoma virus (TrV), a member of this family, infects several species of triatomine insects (popularly named kissing bugs), which are vectors for human trypanosomiasis, more commonly known as Chagas disease. The potential use of dicistroviruses as biological control agents has drawn considerable attention in the past decade, and several viruses of this family have been identified, with their targets covering honey bees, aphids and field crickets, among others. Here, the crystal structure of the TrV capsid at 2.5 Å resolution is reported, showing that as expected it is very similar to that of Cricket paralysis virus (CrPV). Nevertheless, a number of distinguishing structural features support the introduction of a new genus (Triatovirus; type species TrV) under the Dicistroviridae family. The most striking differences are the absence of icosahedrally ordered VP4 within the infectious particle and the presence of prominent projections that surround the fivefold axis. Furthermore, the structure identifies a second putative autoproteolytic DDF motif in protein VP3, in addition to the conserved one in VP1 which is believed to be responsible for VP0 cleavage during capsid maturation. The potential meaning of these new findings is discussed.

  16. Sobre a domiciliaçao dos triatomineos

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    Mário B. Aragão

    1981-06-01

    Full Text Available Foram reunidos resultados de testes de precipitina feitos com o conteúdo do tubo digestivo de triatomineos. €ssas informações foram discutidas com base nas proporções de sangue de mamíferos e de ave, encontradas e em diversas observações relativas às espécies de vertebrados a que os triatomíneos estão associados na natureza. O estudo englobou as seguintes espécies: Belminus peruvianus, Panstrongylus geniculatus, Panstrongilus herreri, Panstrongylus megistus, Psammolestes tertius, Rhodnius neglectus, Rhodnius prolixus,Triatoma arthurneivai, Triatoma dimidiata,Triatoma infestans, Triatoma protracta, Triatoma rubrovaria e Triatoma sórdida. As seguintes conclusões puderam ser obtidas: 1 - As espécies que, nos biótopos artificiais, se alimentam principalmente de sangue humano, na natureza, são as mais ecléticas. Sugam mamífero, ave e animais de sangue-frio, mas apresentam marcada preferência por sangue de mamífero. 2 - As espécies que, nos biótopos artificiais, são encontradas com maior freqüência associadas a aves, na natureza ou preferem sangue de ave ou não tem predileção nítida por ave ou mamífero. 3 - As espécies associadas a animais de sangue frio ou restritas a um único grupo de mamíferos, podem colonizar nos domicílios desde que eles sejam previamente colonizados pelo hospedeiro. 4 - Não foram encontrados indícios de que alguma espécie esteja se adaptando aos domicílios. Todos os triatomineos ditos domiciliários já possuíam, na natureza, atributos que permitiam a vida em novos biótopos, habitados ou pelo homem ou por animais domésticos.

  17. Detoxification of aflatoxin B1 by manganese peroxidase from the white-rot fungus Phanerochaete sordida YK-624.

    Science.gov (United States)

    Wang, Jianqiao; Ogata, Makoto; Hirai, Hirofumi; Kawagishi, Hirokazu

    2011-01-01

    Aflatoxin B(1) (AFB(1) ) is a potent mycotoxin with mutagenic, carcinogenic, teratogenic, hepatotoxic, and immunosuppressive properties. In order to develop a bioremediation system for AFB(1) -contaminated foods by white-rot fungi or ligninolytic enzymes, AFB(1) was treated with manganese peroxidase (MnP) from the white-rot fungus Phanerochaete sordida YK-624. AFB(1) was eliminated by MnP. The maximum elimination (86.0%) of AFB(1) was observed after 48 h in a reaction mixture containing 5 nkat of MnP. The addition of Tween 80 enhanced AFB(1) elimination. The elimination of AFB(1) by MnP considerably reduced its mutagenic activity in an umu test, and the treatment of AFB(1) by 20 nkat MnP reduced the mutagenic activity by 69.2%. (1) H-NMR and HR-ESI-MS analysis suggested that AFB(1) is first oxidized to AFB(1) -8,9-epoxide by MnP and then hydrolyzed to AFB(1) -8,9-dihydrodiol. This is the first report that MnP can effectively remove the mutagenic activity of AFB(1) by converting it into AFB(1) -8,9-dihydrodiol.

  18. Presencia del Triatoma infestans en la ciudad de Lima

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    Abelardo Tejada

    1958-12-01

    Full Text Available 1 . Ha sido descubierto un foco del Triatoma infestans dentro de la zona urbana de la ciudad de Lima, verificándose su presencia en 10 viviendas de un modesto barrio. En tales casas fueron capturados un total de 99 especímenes del referido insecto, entre adultos y ninfas, así como 148 huevos sin eclosionar. 2. El examen del contenido intestinal y las heces en 68 de los triatomas capturados fue negativo para Trypanosoma cruzi. 3. Se supone que este nuevo foco se haya originado a partir de la región Sur del Perú, donde existen muchas localidades infestadas por el T. infestans. 4. Esta sería la primera vez que se verifica la presencia del T. infestans en la ciudad de Lima.

  19. Ecologia de triatomíneos e transmissão do Trypanosoma cruzi, com especial referência ao Brasil

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    Mauro Pereira Barreto

    1976-12-01

    Full Text Available Com base em seu comportamento ecológico e seu grau de relacionamento com o homem e os mamíferos domésticos, comensais e sinantrópicos, os triatomíneos são distribuídos em seis grupos: 1 Triatomíneos tipicamente silvestres, isto é, espécies só encontradas em ecótopos naturais e que nunca freqüentam as habitações humanas e suas dependências. Conseqüentemente, nunca entram em contacto com o homem e os mamíferos domésticos, a não ser acidentalmente quando estes penetram nos focos naturais. Entretanto, podem ter papel maior ou menor na manutenção da enzootia tripanossômica silvestre. Exemplos: Psammolestes coreodes, Psammolestes tertius, Cavernicola pilosa, Triatoma dispar, Triatoma delpontei e muitas outras espécies cujos hábitos são pouco conhecidos. 2 Triatomíneos essencialmente silvestres cujos adultos invadem, com maior ou menor freqüência, as habitações humanas e suas dependências, sem, todavia, aqui se colonizar. Além do papel que têm no ciclo silvestre de transmissão do T. cruzi, podem, ao entrar em contacto com o homem e os mamíferos domésticos e domiciliados suscetíveis, transmitir-lhes a infecção, tanto em áreas silvestres quanto em ecótopos artificiais. Exemplos: Panstrongylus geniculatus, Triatoma rubrovaria,Triatoma arthumeivai, Triatoma patagonica, Triatoma eratyrusiforme, Rhodnius domesticus e muitas outras espécies cujos hábitos são poucos conhecidos. 3 Triatomíneos silvestres em fase inicial de adaptação aos ecótopos artificiais, formando pequenas colônias principalmente no peridomicílio e, mais raramente, na própria habitação humana. Além da importância que têm no ciclo silvestre do T. cruzi, podem trazer a infecção para os ecótopos artificiais e, em determinadas instâncias, participar do ciclo domiciliário do parasita. Exemplos: Rhodnius neglectus, Triatoma vitticeps. Triatoma platensis e outras espécies pouco estudadas. 4 Triatomíneos que se criam indiferentemente em

  20. Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug

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    Maria Luiza S. Mello

    2000-06-01

    Full Text Available The survival of Triatoma infestans and of somatic cells from this species is affected by heat shock. In this study, we examined the cell death responses of male germ line cells from 5th instar nymphs 7 and 30 days after heat shock exposure (40ºC, 1 h. The preparations were stained by the Feulgen reaction and the TUNEL immunocytochemical assay. Apoptosis was elicited by heat shock, with positive TUNEL responses in spermatogonial chromatin and chromosomes, spermatids and sperm cells. Spermatogonia were most affected seven days after the shock whereas some spermatids and sperm cells exhibited DNA fragmentation only thirty days after heat shock. The rate of cell death varied among the cells. In some cases, cellular differentiation was unaffected by heat shock, with DNA fragmentation occurring towards the end of spermatogenesis.Uma vez que em Triatoma infestans a sobrevivência de células somáticas e dos próprios espécimes é afetada por choques de temperatura, foi estudada a indução de morte celular em células da linhagem germinativa masculina de ninfas de 5o. estadio 7 e 30 dias após choque de 1 h a 40ºC. Os preparados foram submetidos à reação de Feulgen e ao teste imunocitoquímico TUNEL. Foi encontrada apoptose induzida pelo choque de temperatura, sendo que respostas positivas ao TUNEL foram vistas na cromatina e em cromossomos de espermatogônias e em espermátides e espermatozóides. As espermatogônias foram afetadas principalmente 7 dias após o choque, enquanto algumas espermátides e espermatozóides exibiram fragmentação de DNA apenas 30 dias após o choque. Admite-se que a velocidade com a qual avança o processo de morte celular varie nessas células. Diferenciação celular não é impedida em parte das células afetadas, permitindo que a fragmentação de seu DNA ocorra mais tardiamente, inclusive no fim da espermatogênese.

  1. Genetic variability of Triatoma flavida and Triatoma bruneri (Hemiptera: Reduviidae by RAPD-PCR technique

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    Jorge Fraga

    2011-02-01

    Full Text Available The Triatominae (Hemiptera:Reduviidae contains the principal and potential Chagas disease vectors present in Mexico, Central America and South America. Triatoma flavida and T. bruneri are Cuban species. These species are closely related according to morphology and were considered synonyms until 1981, when they were separated on the grounds of external characters of the body and the morphology of male genitalia. The present study seeks to analyze genetic polymorphism of T. flavida and T. bruneri populations using RAPD techniques, and to assess the genetic relationship between these species. Ten random primers were used to evaluate the genetic variability among species using RAPD-PCR. The genetic flow among them was calculated. The dendrogram based on calculated Jaccard distances showed two clearly distinguishable clusters which coincided with the studied species. Within each species, moderate genetic differentiation (Fst 0.05-0.15 and migration rates (N > 1 were found among populations, that reveal gene flow and genetic homogeneity. Between species, the Fst value showed a high genetic differentiation and the migration rate was insufficient to maintain genetic homogeneity, and confirmed the absence of gene flow between them. Our results confirm the genetic variability among T. flavida and T. bruneri species.

  2. Genetic variability of Triatoma flavida and Triatoma bruneri (Hemiptera: Reduviidae) by RAPD-PCR technique.

    Science.gov (United States)

    Fraga, Jorge; Rodriguez, Jinnay; Fuentes, Omar; Hernández, Yenin; Castex, Mayda; Gonzalez, Raul; Fernández-Calienes, Aymé

    2011-01-01

    The Triatominae (Hemiptera:Reduviidae) contains the principal and potential Chagas disease vectors present in Mexico, Central America and South America. Triatoma flavida and T. bruneri are Cuban species. These species are closely related according to morphology and were considered synonyms until 1981, when they were separated on the grounds of external characters of the body and the morphology of male genitalia. The present study seeks to analyze genetic polymorphism of T. flavida and T. bruneri populations using RAPD techniques, and to assess the genetic relationship between these species. Ten random primers were used to evaluate the genetic variability among species using RAPD-PCR. The genetic flow among them was calculated. The dendrogram based on calculated Jaccard distances showed two clearly distinguishable clusters which coincided with the studied species. Within each species, moderate genetic differentiation (Fst 0.05-0.15) and migration rates (N > 1) were found among populations, that reveal gene flow and genetic homogeneity. Between species, the Fst value showed a high genetic differentiation and the migration rate was insufficient to maintain genetic homogeneity, and confirmed the absence of gene flow between them. Our results confirm the genetic variability among T. flavida and T. bruneri species.

  3. Ciclo evolutivo do Hepatozoon triatomae (Sporozoa, Haemogregarinidae parasita de triatomíneos Evolution cycle of the Hepatozoon triatomae (Sporozoa, Haemogregarinidae, parasite of triatominea

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    Eduardo Olavo da Rocha e Silva

    1975-09-01

    Full Text Available Relata-se o encontro no Estado de São Paulo, Brasil, de triatomíneos e lagartos, respectivamente o Triatoma arthurneivai e Tropidurus torquatus, parasitados por uma hemogregarina semelhante a Hepatozoon triatomae (Osimani, 1942 Reichenow, 1953. São apresentados aspectos da sua morfologia e estudadas as diversas fases do seu ciclo evolutivo, este inteiramente reproduzido em laboratório.The author relates the discovery of Triatoma arthurneivai and Tropidurus torquatus, in the localities of Lavras de Cima and Santo Antonio, State of São Paulo, Brazil, infected by a hemogregarine similar to Hepatozoon triatomae (Osimani, 1942 Reichenow 1953. He presents certain aspects of its morphology and also studies the several phases of its evolution cycle, which were completed in the laboratory.

  4. Revalidation and redescription of Triatoma brasiliensis macromelasoma Galvão, 1956 and an identification key for the Triatoma brasiliensis complex (Hemiptera: Reduviidae: Triatominae

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    Jane Costa

    2013-09-01

    Full Text Available Triatoma brasiliensis macromelasoma is revalidated based on the results of previous multidisciplinary studies on the Triatoma brasiliensis complex, consisting of crossing experiments and morphological, biological, ecological and molecular analyses. These taxonomic tools showed the closest relationship between T. b. macromelasoma and Triatoma brasiliensis brasiliensis. T. b. macromelasoma is redescribed based on specimens collected in the type locality and specimens from a F1 colony. The complex now comprises T. b. brasiliensis, T. b. macromelasoma, Triatoma melanica, Triatoma juazeirensis and Triatoma sherlocki. An identification key for all members of the complex is presented. This detailed comparative study of the morphological features of T. b. macromelasoma and the remaining members of the complex corroborates results from multidisciplinary analyses, suggesting that the subspecific status is applicable. This subspecies can be distinguished by the following combination of features: a pronotum with 1+1 narrow brownish-yellow stripes on the submedian carinae, not attaining its apex, hemelytra with membrane cells darkened on the central portion and legs with an incomplete brownish-yellow ring on the apical half of the femora. Because the T. brasiliensis complex is of distinct epidemiological importance throughout its geographic distribution, a precise identification of its five members is important for monitoring and controlling actions against Chagas disease transmission.

  5. Revalidation and redescription of Triatoma brasiliensis macromelasoma Galvão, 1956 and an identification key for the Triatoma brasiliensis complex (Hemiptera: Reduviidae: Triatominae)

    Science.gov (United States)

    Costa, Jane; Correia, Nathália Cordeiro; Neiva, Vanessa Lima; Gonçalves, Teresa Cristina Monte; Felix, Márcio

    2013-01-01

    Triatoma brasiliensis macromelasoma is revalidated based on the results of previous multidisciplinary studies on the Triatoma brasiliensis complex, consisting of crossing experiments and morphological, biological, ecological and molecular analyses. These taxonomic tools showed the closest relationship between T. b. macromelasoma and Triatoma brasiliensis brasiliensis. T. b. macromelasoma is redescribed based on specimens collected in the type locality and specimens from a F1 colony. The complex now comprises T. b. brasiliensis, T. b. macromelasoma, Triatoma melanica, Triatoma juazeirensis and Triatoma sherlocki. An identification key for all members of the complex is presented. This detailed comparative study of the morphological features of T. b. macromelasoma and the remaining members of the complex corroborates results from multidisciplinary analyses, suggesting that the subspecific status is applicable. This subspecies can be distinguished by the following combination of features: a pronotum with 1+1 narrow brownish-yellow stripes on the submedian carinae, not attaining its apex, hemelytra with membrane cells darkened on the central portion and legs with an incomplete brownish-yellow ring on the apical half of the femora. Because the T. brasiliensis complex is of distinct epidemiological importance throughout its geographic distribution, a precise identification of its five members is important for monitoring and controlling actions against Chagas disease transmission. PMID:24037202

  6. Phanerochaete sordida YK-624胞外酶的特性及其对纸浆的漂白作用%Properties of Extracellular Enzymes from Phanerochaete Sordida YK-624 and Its Application in KP Bleaching

    Institute of Scientific and Technical Information of China (English)

    谢慧芳; 李忠正; 近藤隆一郎

    2004-01-01

    以Phanerochaete sordida YK-624进行液体培养得到胞外酶液,从纸浆的生物漂白角度考察其酶学性质.对在漂白过程中可能涉及到的锰过氧化物酶(MnP)、过氧化物酶(LiP)、漆酶(Laccase)、木聚糖酶和纤维素酶的活性进行了测定.结果表明,其中主要表现为锰过氧化物酶的活性,未检测到纤维素酶和木聚糖酶的活性.这种酶液进行生物漂白不会造成纤维强度等性质的下降.本文对其中占主要活性成分的锰过氧化物酶的影响因素进行了探讨,发现温度、H2O2浓度、pH值对其活性影响较大,温度对酶液的稳定性也有较大影响.其发挥较佳活性的条件是:温度30℃、H2O2浓度1.0mmol/L、pH值4.5.Mn2+浓度对其活性影响较小.利用液体培养的胞外酶液对几种未漂浆进行了生物漂白,结果表明,在各自最佳条件下,硫酸盐阔叶木浆白度可提高30.4%ISO,碱法杨木/麦草(30/70)混合浆白度可提高17.9%SBD,碱法稻草浆白度可提高12.1%SBD.

  7. Triatoma maculata colonises urban domicilies in Boa Vista, Roraima, Brazil

    Science.gov (United States)

    Ricardo-Silva, Alice; Gonçalves, Teresa Cristina Monte; Luitgards-Moura, José Francisco; Lopes, Catarina Macedo; da Silva, Silvano Pedrosa; Bastos, Amanda Queiroz; Vargas, Nathalia Coelho; Freitas, Maria-Rosa Goreti

    2016-01-01

    During a medical entomology course in Boa Vista, Roraima, colonies of Triatoma maculata closely associated with pigeon nests were observed in concrete air-conditioner box located on the external plastered and cemented walls of a modern brick-built apartment block. In only one eight-hole ceramic brick, located inside one air-conditioner box, 127 specimens of T. maculata were collected. T. maculata is a recognised vector of Trypanosoma cruzi in the surrounding area and its domiciliation increases the risk of Chagas disease transmission. PMID:27759767

  8. Temperature effect upon blood consumption in Triatoma infestans

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    Silvia Catalá

    1992-12-01

    Full Text Available Different blood consumption speed was observed in Triatoma infestans - nymphs and adults - exposed to 12 degrees C and 28 degrees C. Exposure to optimal temperature (28 degrees C allows the insects to consume blood at a rate of 9% per day. Significative relationship between blood amount present in the promesenteron and consumed blood was found at 28 degrees. Consumption of blood was drastically reduced at the lowest temperature. Accordingly, lack of ovaric development, oviposition and mating behaviour was observed in insects kept at 12 degrees C. Relationship between laboratory and field observations are discussed.

  9. Phylogeography and genetic variation of Triatoma dimidiata, the main Chagas disease vector in Central America, and its position within the genus Triatoma.

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    María Dolores Bargues

    Full Text Available BACKGROUND: Among Chagas disease triatomine vectors, the largest genus, Triatoma, includes species of high public health interest. Triatoma dimidiata, the main vector throughout Central America and up to Ecuador, presents extensive phenotypic, genotypic, and behavioral diversity in sylvatic, peridomestic and domestic habitats, and non-domiciliated populations acting as reinfestation sources. DNA sequence analyses, phylogenetic reconstruction methods, and genetic variation approaches are combined to investigate the haplotype profiling, genetic polymorphism, phylogeography, and evolutionary trends of T. dimidiata and its closest relatives within Triatoma. This is the largest interpopulational analysis performed on a triatomine species so far. METHODOLOGY AND FINDINGS: Triatomines from Mexico, Guatemala, Honduras, Nicaragua, Panama, Cuba, Colombia, Ecuador, and Brazil were used. Triatoma dimidiata populations follow different evolutionary divergences in which geographical isolation appears to have had an important influence. A southern Mexican-northern Guatemalan ancestral form gave rise to two main clades. One clade remained confined to the Yucatan peninsula and northern parts of Chiapas State, Guatemala, and Honduras, with extant descendants deserving specific status. Within the second clade, extant subspecies diversity was shaped by adaptive radiation derived from Guatemalan ancestral populations. Central American populations correspond to subspecies T. d. dimidiata. A southern spread into Panama and Colombia gave the T. d. capitata forms, and a northwestern spread rising from Guatemala into Mexico gave the T. d. maculipennis forms. Triatoma hegneri appears as a subspecific insular form. CONCLUSIONS: The comparison with very numerous Triatoma species allows us to reach highly supported conclusions not only about T. dimidiata, but also on different, important Triatoma species groupings and their evolution. The very large intraspecific genetic

  10. Phylogeography and Genetic Variation of Triatoma dimidiata, the Main Chagas Disease Vector in Central America, and Its Position within the Genus Triatoma

    Science.gov (United States)

    Bargues, María Dolores; Klisiowicz, Debora R.; Gonzalez-Candelas, Fernando; Ramsey, Janine M.; Monroy, Carlota; Ponce, Carlos; Salazar-Schettino, Paz María; Panzera, Francisco; Abad-Franch, Fernando; Sousa, Octavio E.; Schofield, Christopher J.; Dujardin, Jean Pierre; Guhl, Felipe; Mas-Coma, Santiago

    2008-01-01

    Background Among Chagas disease triatomine vectors, the largest genus, Triatoma, includes species of high public health interest. Triatoma dimidiata, the main vector throughout Central America and up to Ecuador, presents extensive phenotypic, genotypic, and behavioral diversity in sylvatic, peridomestic and domestic habitats, and non-domiciliated populations acting as reinfestation sources. DNA sequence analyses, phylogenetic reconstruction methods, and genetic variation approaches are combined to investigate the haplotype profiling, genetic polymorphism, phylogeography, and evolutionary trends of T. dimidiata and its closest relatives within Triatoma. This is the largest interpopulational analysis performed on a triatomine species so far. Methodology and Findings Triatomines from Mexico, Guatemala, Honduras, Nicaragua, Panama, Cuba, Colombia, Ecuador, and Brazil were used. Triatoma dimidiata populations follow different evolutionary divergences in which geographical isolation appears to have had an important influence. A southern Mexican–northern Guatemalan ancestral form gave rise to two main clades. One clade remained confined to the Yucatan peninsula and northern parts of Chiapas State, Guatemala, and Honduras, with extant descendants deserving specific status. Within the second clade, extant subspecies diversity was shaped by adaptive radiation derived from Guatemalan ancestral populations. Central American populations correspond to subspecies T. d. dimidiata. A southern spread into Panama and Colombia gave the T. d. capitata forms, and a northwestern spread rising from Guatemala into Mexico gave the T. d. maculipennis forms. Triatoma hegneri appears as a subspecific insular form. Conclusions The comparison with very numerous Triatoma species allows us to reach highly supported conclusions not only about T. dimidiata, but also on different, important Triatoma species groupings and their evolution. The very large intraspecific genetic variability found in T

  11. Ampliação da distribuição geográfica de Triatoma deaneorum Galvão, Souza & Lima 1967, nova denominação para Triatoma deanei (Hemiptera, Reduviidae New geographical distribution of Triatoma deaneorum Galvão, Souza & Lima 1967, new designation for Triatoma deanei (Hemiptera, Reduviidae

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    Cleber Galvão

    2001-12-01

    Full Text Available A distribuição geográfica de Triatoma deaneorum Galvão, Souza & Lima, 1967, nova denominação para Triatoma deanei, é ampliada baseada em um espécime recentemente encontrado no município de Chapada de Guimarães, próximo a Cuiabá, Mato Grosso, Brasil. A nova denominação é baseada no Código Internacional de Nomenclatura Zoológica.Authors extend the geographical distribution of Triatoma deaneorum Galvão, Souza & Lima, 1967, new designation for Triatoma deanei, based on a specimen recently found in Chapada dos Guimarães, near to Cuiabá, Mato Grosso, Brazil. The new designation is based on the International Code of Zoological Nomenclature.

  12. Descrição de ovos e ninfas de Triatoma klugi (Hemiptera, Reduviidae, Triatominae Description of eggs and nymphs of Triatoma klugi (Hemiptera, Reduviidae, Triatominae

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    José Jurberg

    Full Text Available Os autores descreveram as características morfológicas de Triatoma klugi Carcavallo, Jurberg, Lent & Galvão, 2001 pertencente ao grupo de espécies que compõem o "complexo T. oliveirai". Até o presente tem sido difícil separar essas espécies com base nas características ninfais, o que justifica o desenvolvimento deste trabalho. Os espécimes foram coletados em frestas de rochas no morro Malavok na localidade de Linha Brasil, município de Nova Petrópolis, no Estado do Rio Grande do Sul. O local de captura dos triatomíneos situa-se entre 700 e 800 m de altitude (29º18'38''S, 51º04'57''W. Juntamente com T. oliveirai (Neiva, Pinto & Lent, 1939, espécie morfologicamente mais próxima, são as únicas do complexo que não foram encontradas, até o momento, no Estado do Mato Grosso, Mato Grosso do Sul e Goiás, Brasil. Em condições experimentais, já foi testada e comprovada a sua susceptibilidade à infecção pelo Trypanosoma cruzi (Chagas, 1909 e Trypanosoma rangeli (Tejera, 1920. A análise comparativa das ornamentações do exocório dos ovos e de três estádios ninfais de T. klugi por microscopia óptica e microscopia eletrônica de varredura mostrou algumas particularidades morfológicas, com destaque para: a face ventral da cabeça, o sulco estridulatório e os últimos segmentos abdominais (IX, X - futura genitália e XI - tubo anal. Esses dados contribuem para a ampliação dos parâmetros diferenciais visando à diagnose de T. klugi durante o seu desenvolvimento ninfal.The authors had described the morphologic characteristics of Triatoma klugi Carcavallo, Jurberg, Lent & Galvão, 2001 to the group of species belonging to "T. oliveirai complex". Until the moment it has been difficult to separate on the nymphs characteristics basis these species, what it justifies the development of this work. The specimens had been collected in openings of rocks in the Malavok mount in the locality of Linha Brazil, city of Nova Petr

  13. Ecología urbana de Triatoma infestans en Argentina: asociación entre Triatoma infestans y palomares Triatoma infestans urban ecology in Argentina: association between Triatoma infestans and pigeon cotes

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    Silvana L. Vallvé

    1995-06-01

    Full Text Available Estudio se realizó en una área urbana de la Capital de la Provincia de San Juan, Argentina, en un barrio de 768 apartamentos distribuidos en edificios de 3 y 7 pisos que rodean una bodega central abandonada. Se capturaron 329 ejemplares de Triatoma infestans, 293 en 4 terrazas de torres de 7 pisos y 36 en el interior de la bodega, associados con numerosas palomas que colomizaban estos sitios. Los insectos se refugiaban en el guano acumulado entre bloques de cemento que cubren las terrazas y en la bodega dentro de toneles en desuso. Se identificaron dos focos principales de vectores associados con densos palomares: un 81,5% del total de triatomas colectados se halló en una de las torres y un 11% en la bodega. A los 6 meses del tratamiento insecticida, resultaron positivas terrazas en las que previamente no se habia capturado T. infestans. El perfil alimentario de los triatomas muestra un predominio de ingestas simples sobre ave; en los edificios y en la bodega el 95% de los T. infestans analizados se alimentó sólo de ave; el resto fueron ingestas simples o dobles sobre humano, perro o gato. Ningún T. infestans presentó infección con Trypanosoma cruzi.Estudo realizado em local urbano da capital da Província de San Juan, Argentina, num bairro de 768 apartamentos distribuídos em prédios de 3 e 7 andares que cingem uma adega vinícola abandonada. Foram coletados 329 exemplares de Triatoma infestans, 293 em 4 terraços das torres de 7 andares e 36 no interior da adega, associados às numerosas pombas que colonizavam esses sítios. Os insetos refugiavam-se no excremento cumulado entre os blocos de cimento que cobrem os terraços e, na adega, dentro de tonéis fora de uso. Foram identificados dois focos principais de vetores, associados com densos pombais: 81,5% do total de triatomas coletados foram apanhados em uma das torres e 11% na adega. Após 6 meses de tratamento com inseticida, foram positivos os terraços onde anteriormente não se

  14. Periurban Trypanosoma cruzi-infected Triatoma infestans, Arequipa, Peru.

    Science.gov (United States)

    Levy, Michael Zachary; Bowman, Natalie M; Kawai, Vivian; Waller, Lance A; Cornejo del Carpio, Juan Geny; Cordova Benzaquen, Eleazar; Gilman, Robert H; Bern, Caryn

    2006-09-01

    In Arequipa, Peru, vectorborne transmission of Chagas disease by Triatoma infestans has become an urban problem. We conducted an entomologic survey in a periurban community of Arequipa to identify risk factors for triatomine infestation and determinants of vector population densities. Of 374 households surveyed, triatomines were collected from 194 (52%), and Trypanosoma cruzi-carrying triatomines were collected from 72 (19.3%). Guinea pig pens were more likely than other animal enclosures to be infested and harbored 2.38x as many triatomines. Stacked brick and adobe enclosures were more likely to have triatomines, while wire mesh enclosures were protected against infestation. In human dwellings, only fully stuccoed rooms were protected against infestation. Spatially, households with triatomines were scattered, while households with T. cruzi-infected triatomines were clustered. Keeping small animals in wire mesh cages could facilitate control of T. infestans in this densely populated urban environment.

  15. Behavioural Response of Triatoma infestans (Klug (Hemiptera: Reduviidae to Quinazolines

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    E. Zerba

    2005-09-01

    Full Text Available The behavioural responses of the haematophagous bug Triatoma infestans towards some previously identified components of its faeces: 4-methylquinazoline, 2,4- dimethylquinazoline and their mixtures were evaluated using a video tracking system. Fifth instar nymphs and females but not males were significantly attracted to polyethylene glycol formulations of 4-methyl + 2,4-dimethylquinazoline (50 μg each. Fifth instar nymphs were also attracted to 4-methylquinazoline alone (50 μg but females were only attracted by the mixture of both methyl quinazolines (50 μg each. Syntheses of both methyl quinazolines were carried out starting from 2-aminoacetophenone by modifying the conditions of reported procedures.

  16. Influência de hábitos antrópicos na dispersão de Triatoma pseudomaculata Corrêa & Espínola, 1964, através de Mimosa tenuiflora (Willdenow) (Mimosaceae) no Estado do Ceará, Brasil Influence of anthropic habits in the dispersion of Triatoma pseudomaculata Corrêa & Espínola, 1964 through Mimosa tenuiflora (Willdenow) (Mimosaceae) in the State of Ceará, Brazil

    OpenAIRE

    Simone Patrícia Carneiro Freitas; Assilon Lindoval Carneiro Freitas; Severino do Monte Prazeres; Teresa Cristina Monte Gonçalves

    2004-01-01

    Triatoma pseudomaculata foram capturados em entrecascas de Mimosa tenuiflora (jurema-preta) em ecótopo silvestre de sete municípios do Estado do Ceará. Esta planta apresenta alta taxa de cobertura geográfica nas áreas de caatingas, sendo freqüentemente utilizada pela população rural. A presença de triatomíneos no peridomicílio, na região do Cariri, pode estar associada ao uso pela população da madeira de M. tenuiflora, quando trazida do ambiente silvestre, repleta de triatomíneos, facilitando...

  17. Fontes alimentares de Triatoma pseudomaculata no Estado do Ceará, Brasil

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    Simone Patrícia Carneiro Freitas

    2005-01-01

    Full Text Available OBJETIVO: Triatoma pseudomaculata, espécie peridomiciliar, é encontrada apresentando baixa taxa de infecção por Trypanosoma cruzi. Com o objetivo de identificar os possíveis reservatórios de T. cruzi, investigou-se a ocorrência desse triatomíneo no domicílio, bem como suas fontes alimentares. MÉTODOS: De janeiro de 2001 a julho de 2002 foram capturados 921 espécimes de T. pseudomaculata em 13 municípios do sul do Estado do Ceará. O conteúdo intestinal dos triatomíneos foi retirado, espalhado em disco de papel de filtro e analisado por precipitina para os seguintes anti-soros: ave, roedor, cão, gambá, lagarto, boi/cabra, gato, porco, barata e humano. A investigação da presença de T. cruzi foi feita observando-se parte do conteúdo intestinal dos insetos a fresco, entre lâmina e lamínula, e pela sua semeadura em meio de cultura. RESULTADOS: Do total examinado, 184 (90,6% foram positivos para os anti-soros testados: ave (62,5%> roedor (33,7%> cão (20,1%> gambá (9,8%> lagarto (5%> boi-cabra (5%> gato (2,7%> porco (2,2%> barata (2,2%> humano (1,6%. As alimentações variaram de zero (não reagiram a quatro da seguinte forma: não reagiram (9,4%, uma (57,1%, duas (26%, três (7% ou quatro (0,5%. Das fontes alimentares identificadas apenas três espécimes (1,6% foram positivos para T. cruzi. CONCLUSÕES: A baixa incidência de sangue humano mostra que T. pseudomaculata está bem adaptado ao peridomicílio. Porém, a vigilância epidemiológica na região sul do Estado do Ceará se faz necessária tendo em vista a proximidade da espécie ao domicílio.

  18. Triatoma jatai sp. nov. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae

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    Teresa Cristina Monte Goncalves

    2013-06-01

    Full Text Available Triatoma jatai sp. nov. is the first new species of triatomine to be described in the state of Tocantins, in the northern region of Brazil. It was caught on rock outcrops in the wild environment and, more recently, invading homes. While T. jatai sp. nov. is morphologically similar to Triatoma costalimai, it is distinguished by its general colouring, differences in the blotches on the connexivum, wing size in females and external structures of the male genitalia. The type series has been deposited in the Entomological Collection and Herman Lent Collection, Oswaldo Cruz Institute-Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

  19. Triatoma jatai sp. nov. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae)

    Science.gov (United States)

    Gonçalves, Teresa Cristina Monte; Teves-Neves, Simone Caldas; dos Santos-Mallet, Jacenir Reis; Carbajal-de-la-Fuente, Ana Laura; Lopes, Catarina Macedo

    2013-01-01

    Triatoma jatai sp. nov. is the first new species of triatomine to be described in the state of Tocantins, in the northern region of Brazil. It was caught on rock outcrops in the wild environment and, more recently, invading homes. While T. jatai sp. nov. is morphologically similar to Triatoma costalimai, it is distinguished by its general colouring, differences in the blotches on the connexivum, wing size in females and external structures of the male genitalia. The type series has been deposited in the Entomological Collection and Herman Lent Collection, Oswaldo Cruz Institute-Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. PMID:23828010

  20. Avaliação de óleos essenciais de plantas aromáticas com atividade inseticida em Triatoma infestans (Klug, 1834 (Hemiptera: Reduviidae = Evaluation of essential oils with insecticidal activity in Triatoma infestans (Klug, 1834 (Hemiptera: Reduviidae

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    Suellem Petilim Gomes

    2011-07-01

    Full Text Available A doenca de Chagas e um dos maiores problemas de saude publica na America Latina. Neste trabalho, foi avaliada a acao inseticida de oleos essenciais de Anacardium humile Cymbopogon nardus, Ocimum basilicum e Eucalyptus urograndis em ninfas do 3‹ e 4‹ estadios dedesenvolvimento de Triatoma infestans por acao topica e pressao a vapor (fumigacao. Para o teste topico foram realizadas duas repeticoes cada uma com cinco ninfas de 3‹ e 4‹ estadios de desenvolvimento de T. infestans. Foi aplicado 1ƒÊL de cada concentracao na regiao dorsal de cada individuo. Para o teste de fumigacao foram utilizados potes de 2 L com tampa vedante. Os oleos essenciais de O. gratissimum e E. urograndis apresentaram atividadeinseticida para T. infestans, e A. humile e C. nardus apresentaram em media 5 e 15% de mortalidade, respectivamente. Para fumigacao, somente o oleo de E. urograndis teve efeito, com 100% de mortalidade sobre T. infestans , sendo que para os outros oleos nao foiobservado efeito de fumigacao. Esse trabalho relata pela primeira vez a acao inseticida de E. urograndis em potes fumigenos para o controle de triatomineos. Os resultados sao importantes devido a busca por novos modelos moleculares com acao inseticida em triatomineos resistentes aos inseticidas sinteticos.Chagas disease is a major public health problem in Latin America. This work aimed to evaluate the insecticide action of essential oils of Anacardium humile, Cymbopogon nardus, Ocimum basilicum and Eucalyptus urograndis on Triatoma infestans nymphs of third and fourth stages of development by topic action and steam pressure (fumigation. Two repetitions were performed at the topic test with five nymphs of third and fourth stages of development of T. infestans. It was applied 1ƒÊL of each concentration on the dorsal region of each nymph. Two-liter pots with sealing covers were used at the fumigation test. The essential oils of O. gratissimum and E. urograndis showed insecticidal

  1. Fontes alimentares de Triatoma pseudomaculata no Estado do Ceará, Brasil Feeding patterns of Triatoma pseudomaculata in the state of Ceará, Brazil

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    Simone Patrícia Carneiro Freitas

    2005-01-01

    Full Text Available OBJETIVO: Triatoma pseudomaculata, espécie peridomiciliar, é encontrada apresentando baixa taxa de infecção por Trypanosoma cruzi. Com o objetivo de identificar os possíveis reservatórios de T. cruzi, investigou-se a ocorrência desse triatomíneo no domicílio, bem como suas fontes alimentares. MÉTODOS: De janeiro de 2001 a julho de 2002 foram capturados 921 espécimes de T. pseudomaculata em 13 municípios do sul do Estado do Ceará. O conteúdo intestinal dos triatomíneos foi retirado, espalhado em disco de papel de filtro e analisado por precipitina para os seguintes anti-soros: ave, roedor, cão, gambá, lagarto, boi/cabra, gato, porco, barata e humano. A investigação da presença de T. cruzi foi feita observando-se parte do conteúdo intestinal dos insetos a fresco, entre lâmina e lamínula, e pela sua semeadura em meio de cultura. RESULTADOS: Do total examinado, 184 (90,6% foram positivos para os anti-soros testados: ave (62,5%> roedor (33,7%> cão (20,1%> gambá (9,8%> lagarto (5%> boi-cabra (5%> gato (2,7%> porco (2,2%> barata (2,2%> humano (1,6%. As alimentações variaram de zero (não reagiram a quatro da seguinte forma: não reagiram (9,4%, uma (57,1%, duas (26%, três (7% ou quatro (0,5%. Das fontes alimentares identificadas apenas três espécimes (1,6% foram positivos para T. cruzi. CONCLUSÕES: A baixa incidência de sangue humano mostra que T. pseudomaculata está bem adaptado ao peridomicílio. Porém, a vigilância epidemiológica na região sul do Estado do Ceará se faz necessária tendo em vista a proximidade da espécie ao domicílio.OBJECTIVE: T. pseudomaculata, a peridomicilar species, has low rates of T. cruzi infection. The occurrence of this triatomine in the domicile and its feeding patterns was investigated to identify potential T. cruzi reservoirs. METHODS: Nine-hundred and twenty-one specimens of T. pseudomaculata were captured from January 2001 to July 2002 in 13 southern municipalities of the state

  2. Medida da infestação domiciliar por Triatoma infestans Housing infestation research by Triatoma infestans

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    Oswaldo Paulo Forattini

    1969-06-01

    Full Text Available Em conjunto de 27 edifícios situados na localidade denominada Bairro da Ilha, no Município de Salto de Pirapora, Estado de São Paulo, procurou-se aplicar o método de Gómez-Núñez para a seleção de casas infestadas pelo Triatoma infestans. Comparando com o processo de inspeção domiciliar mediante a captura manual, porém sem o emprêgo de substâncias insetífugas, o método revelou-se equivalente. Contudo, êle traz vantagens que não podem ser desprezadas. Entre outras, ressaltam a sua uniformidade e o baixo custo. Através o levantamento da percentagem de caixas positivas até a segunda semana de observação, pode-se também estimar o grau de infestação local. Nesta pesquisa, revelou índice de Tipo III. O significado e classificação desses índices deverá ser objeto de maiores investigações. Contudo, no que pese a necessidade de maior número de ensaios, o método poderá vir a ser de bastante utilidade na avaliação de localidades submetidas a expurgos, sejam êles seletivos ou não.In 27 dwellings located at Bairro da Ilha, in the County of Salto de Pirapora, S. Paulo State, Brazil, it the Gómez-Núñez method for selection of houses infested by Triatoma infestans bugs was tested. In the observations there no significant differences between this method and the simple house inspection through manual collection, were found but without using chemicals as repellents. Nevertheless, several good advantages such as the uniformity and the low cost of the method were noticed. The purpose was also to estimate the local infestation through the infestation index based on the percentage of positive boxes found in the second week of observation. In this research, the index was classified as Type III. The significance and usefulness of this classification will be tested by future researches. Nevertheless it will be reasonable to admit that the method has good possibilities of being used for the houses evaluation, after the insecticide

  3. Nota sobre o uso de bio-sensores "Maria" nas ações de vigilância entomológica contra a doença de Chagas ao norte de Minas Gerais About the use of "Maria" bio-sensors in the activities of epidemiological surveillance against Chagas' disease in the north of Minas Gerais State

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    João Carlos Pinto Dias

    2005-10-01

    Full Text Available Oitocentos e sessenta e seis bio-sensores para a detecção passiva de triatomíneos foram ensaiados em intradomicílios de treze municípios de área endêmica de Triatoma sordida (Norte de Minas Gerais, Brasil, espécie que é hoje a mais freqüentemente detectável no Brasil, especialmente naquela Região. Examinados os sensores a cada três meses, por quatro vezes em uma subárea de sete municípios (seiscentos e quarenta e dois sensores, com positividade máxima de 0,5% e por duas nos outros seis municípios (duzentos e vinte e quatro sensores, com positividade máxima de 2,7%, os resultados foram, significativamente inferiores à rotina de busca direta hora-homem feita nos mesmos municípios, inclusive para as taxas de infestação intradomiciliar. Em que pese a simplicidade e boa aceitação dos sensores pela população, os mesmos não se mostraram adequados à pesquisa triatomínica na região em apreço, tanto em termos de efetividade quanto de custo-benefício.Eight hundred and sixty six bio-sensors for the passive detection of triatomine bugs, were tried in intra-domiciles of thirteen municipalities of an endemic area of Triatoma sordida in the North of Minas Gerais State, Brazil. This species is nowadays the most captured in Brazil and mainly in the above region. The sensors were examined every three months, corresponding to four times in the first seven municipalities (six hundred and forty two sensors, with a maximum positivity of 0.5% and two times in the other six (two hundred and twenty two sensors, with a maximum positivity of 2.7%, showing results significantly inferior in comparison with the routine man- hour research performed in the same municipalities, including in terms of intra-domestic infestation index. Despite the simplicity and good acceptance of the sensors by the population they were demonstrated to be inadequate for triatomine research under the conditions of this trial, in terms of efficacy and cost-benefit.

  4. Cytogenetics of Triatominae: III - A study on male sterility induced through hybridization of Triatoma sórdida and Triatoma pseudomaculata

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    Giorgio Schreiber

    1975-08-01

    Full Text Available Males from bilateral crosses between Triatoma sórdida and Triatoma pseudomaculata were unable to give offspring, as shown by subsequent backcrosses (BC between hybrid males and parental females. This kind of sterility indueed through interspecific hybridization seems to be due to lack of sperm migration from the bursa copulatrix to the spermateca, thus suggesting primarily failure on the part of hybrid males to produce and/or to incorporate male accessory secretions into the spermatophore bulb. Addicional proof that sterility induced in hybrid males is at the sperm level has been afforded by the spermatogenesis herein studied. The anomalous processes like; 1 prophases of spermatogonia with the chromosomes scattered in the cytoplasm, 2 first metaphases with unpaired tetrades, 3 spermatids differing in size and 4 spermatozoa of abnormal shape and generdlly of giant size, can be taken as an indicator of the degree of departure from the normal course of spermatogenesis.

  5. Estadísticos poblacionales de Triatoma rubrovaria en condiciones de laboratorio Population statistics of Triatoma rubrovaria in laboratory

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    Elena B Oscherov

    2005-04-01

    Full Text Available OBJETIVO: Obtener parámetros poblacionales de T. rubrovaria a fin de caracterizar demográficamente a esta especie. MÉTODOS: La investigación se realizó entre octubre de 2000 y febrero de 2003 en el laboratorio de artrópodos, Corrientes, Argentina. Se conformaron cinco cohortes de 100 huevos cada una. Los insectos se alimentaron sobre gallina (Gallus domesticus. Las cohortes se controlaron semanalmente. La experiencia se llevó a cabo en condiciones controladas de temperatura (28±3ºC y humedad relativa del aire (63±10%. Se elaboraron tablas completas de vida y se obtuvieron estadísticos vitales. RESULTADOS: La mayor mortalidad se registró en ninfas de primero a cuarto estadio. A partir del quinto estadio el número de individuos decreció en forma constante. La expectativa de vida, después de superar las edades críticas, disminuyó en forma lineal. La supervivencia media de los adultos fue 50,2 semanas. La primera oviposición ocurrió a las 40,6 semanas. La fecundidad fue 859,6 huevos, con una media de 22,8 huevos. El período reproductivo fue de 37,7 semanas. El tiempo generacional fue de 55,3 semanas y la tasa neta de reproducción 133,7. La tasa intrínseca de incremento natural resultó 0,088. En una distribución estable de edades 25,3% correspondería al estado de huevo, 72,3% al estado ninfal y 2,4% al estado adulto. Los adultos contribuyeron con más del 70% al valor reproductivo total. CONCLUSIONES: Triatoma rubrovaria se caracterizó por una supervivencia prolongada como imago, una edad de la primera reproducción tardía y una tasa intrínseca de incremento natural baja.OBJECTIVE: To obtain T. rubrovaria population parameters in order to describe its demographic characteristics. METHODS: The study was carried out in the laboratory of Arthropods, Corrientes, Argentina, from October 2000 to February 2003. Eggs were grouped to form five 100-egg cohorts. Insects were fed on chickens (Gallus domesticus. The cohorts were

  6. Influência de hábitos antrópicos na dispersão de Triatoma pseudomaculata Corrêa & Espínola, 1964, através de Mimosa tenuiflora (Willdenow (Mimosaceae no Estado do Ceará, Brasil Influence of anthropic habits in the dispersion of Triatoma pseudomaculata Corrêa & Espínola, 1964 through Mimosa tenuiflora (Willdenow (Mimosaceae in the State of Ceará, Brazil

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    Simone Patrícia Carneiro Freitas

    2004-02-01

    Full Text Available Triatoma pseudomaculata foram capturados em entrecascas de Mimosa tenuiflora (jurema-preta em ecótopo silvestre de sete municípios do Estado do Ceará. Esta planta apresenta alta taxa de cobertura geográfica nas áreas de caatingas, sendo freqüentemente utilizada pela população rural. A presença de triatomíneos no peridomicílio, na região do Cariri, pode estar associada ao uso pela população da madeira de M. tenuiflora, quando trazida do ambiente silvestre, repleta de triatomíneos, facilitando conseqüentemente sua mobilização e dispersão.Triatoma pseudomaculata was captured in the phloem of the black acacia bush (Mimosa tenuiflora in seven municipalities in the State of Ceará, Brazil. This bush is widespread in the caatinga (scrub forest and is used extensively by the rural population for kindling. Peridomiciliary presence of triatomines in the Cariri region may be associated with the use of infested M. tenuiflora wood by the population, thus facilitating the triatomines' mobilization and dispersion.

  7. Cytogenetics of Triatominae: III - A study on male sterility induced through hybridization of Triatoma sórdida and Triatoma pseudomaculata

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    Giorgio Schreiber

    1975-08-01

    Full Text Available Males from bilateral crosses between Triatoma sórdida and Triatoma pseudomaculata were unable to give offspring, as shown by subsequent backcrosses (BC between hybrid males and parental females. This kind of sterility indueed through interspecific hybridization seems to be due to lack of sperm migration from the bursa copulatrix to the spermateca, thus suggesting primarily failure on the part of hybrid males to produce and/or to incorporate male accessory secretions into the spermatophore bulb. Addicional proof that sterility induced in hybrid males is at the sperm level has been afforded by the spermatogenesis herein studied. The anomalous processes like; 1 prophases of spermatogonia with the chromosomes scattered in the cytoplasm, 2 first metaphases with unpaired tetrades, 3 spermatids differing in size and 4 spermatozoa of abnormal shape and generdlly of giant size, can be taken as an indicator of the degree of departure from the normal course of spermatogenesis.Os machos provenientes de cruzamentos bilaterais entre Triatoma sórdida e Triatoma pseudomaculata revelaram a incapacidade de produzir progênie, como se demonstra nos retrocruzamentos de machos híbridos com fêmeas das espécies paternas. Esta modalidade de esterilidade induzida através da hibridação interespecífica, parece ser devida à ausência de migração do esperma, determinada pela incapacidade dos híbridos machos produzirem a secreção opaca em suas glândulas acessórias e/ou incorporá-la à luz do espermatóforo. A prova adicional de que a incompatibilidade induzida em triatomíneos, através da hibridação inter específica, está no nível do esperma é fornecida pelo tipo anômalo de espermatogênese em híbridos aqui descrito, mostrando deste modo que: as prófases espermatogoniais têm os núcleos disruptos e os cromossomos espelhados no citoplasma. As primeiras metáfases meióticas têm as tétrades desemparelhadas. Os grupos de cromatídes assim obtidos

  8. Ciclo biológico de Triatoma rubrovaria (Blanchard (Reduviidae, Triatominae en laboratorio

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    Nilda V. de Argüello

    1988-01-01

    Full Text Available Fez-se uma avaliação estatística do ciclo de vida de Triatoma rubrovaria, com base em experimentos de laboratório. Foram computados a eclosão do ovo, o tempo de desenvolvimento da ninfa e sua mortalidade, e a longevidade dos adultos, machos e fêmeas; os resultados foram comparados com os da literatura existente.A statistical evaluation of the life cycle of Triatoma rubrovaria is based on cohort experiments conducted under controlled laboratory conditions. Egg hatching, nymphal development time and mortality, adult male and femals longevity were computed, and the results compared with information in the existing literature.

  9. Population differentiation of the Chagas disease vector Triatoma maculata (Erichson, 1848) from Colombia and Venezuela.

    Science.gov (United States)

    Monsalve, Yoman; Panzera, Francisco; Herrera, Leidi; Triana-Chávez, Omar; Gómez-Palacio, Andrés

    2016-06-01

    The emerging vector of Chagas disease, Triatoma maculata (Hemiptera, Reduviidae), is one of the most widely distributed Triatoma species in northern South America. Despite its increasing relevance as a vector, no consistent picture of the magnitude of genetic and phenetic diversity has yet been developed. Here, several populations of T. maculata from eleven Colombia and Venezuela localities were analyzed based on the morphometry of wings and the mitochondrial NADH dehydrogenase subunit 4 (ND4) gene sequences. Our results showed clear morphometric and genetic differences among Colombian and Venezuelan populations, indicating high intraspecific diversity. Inter-population divergence is suggested related to East Cordillera in Colombia. Analyses of other populations from Colombia, Venezuela, and Brazil from distinct eco-geographic regions are still needed to understand its systematics and phylogeography as well as its actual role as a vector of Chagas disease.

  10. Ecology of Triatoma rubrovaria (Hemiptera, Triatominae) in wild and peridomestic environments of Uruguay

    OpenAIRE

    Roberto Salvatella; Raquel Rosa; Yester Basmadjian; Andres Puime; Luis Calegari; Jorge Guerrero; Maria Martinez; Gabriela Mendaro; Daniel Briano; Carlos Montero; Cristina Wisnivesky-Colli

    1995-01-01

    This paper describes population structure, spatial distribution and habitat selection of wild and peridomestic populations of Triatoma rubrovaria (Blanchard, 1843). Field studies were carried out at Las Piedras and La Bolsa in the Northern Department of Artigas, Uruguay. A semicircular sampling area, divided in seven or eight triangular sectors was sequentially examined from October 1990 to November 1991. At Las Piedras (typical wild habitat) 1063 T. rubrovaria bugs were collected from 84 of ...

  11. Estudo morfológico de Triatoma guazu Lent & Wygodzinsky, 1979 (Hemiptera, Reduviidae, Triatominae

    Directory of Open Access Journals (Sweden)

    Galindez-Girón Itamar

    1997-01-01

    Full Text Available A female of Triatoma guazu Lent & Wygodzinsky, 1979, a rare species from which the allotype was recently described, was studied by scanning electronic microscopy and videomicroscope. Some structures of the head and the thorax are shown. Some of them could have taxonomic importance, as the oculo-ocellar region, the buccula, the anterolateral angle of the collar, the scutellum, wings and the stridulatory sulcus, which has an unusual lateral high rim

  12. Evolutionary and dispersal history of Triatoma infestans, main vector of Chagas disease, by chromosomal markers

    OpenAIRE

    Panzera, F.; Ferreiro, M. J.; Pita, S.; Calleros, L.; Perez, R.; Basmadjian, Y.; Guevara, Y.; Brenière, Simone Frédérique; Panzera, Y.

    2014-01-01

    Chagas disease, one of the most important vector-borne diseases in the Americas, is caused by Trypanosoma cruzi and transmitted to humans by insects of the subfamily Triatominae. An effective control of this disease depends on elimination of vectors through spraying with insecticides. Genetic research can help insect control programs by identifying and characterizing vector populations. In southern Latin America, Triatoma infestans is the main vector and presents two distinct lineages, known ...

  13. Avaliação de óleos essenciais de plantas aromáticas com atividade inseticida em Triatoma infestans (Klug, 1834 (Hemiptera: Reduviidae Evaluation of essential oils with insecticidal activity in Triatoma infestans (Klug, 1834 (Hemiptera: Reduviidae

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    Silvio Favero

    2011-09-01

    Full Text Available A doença de Chagas é um dos maiores problemas de saúde pública na América Latina. Neste trabalho, foi avaliada a ação inseticida de óleos essenciais de Anacardium humile Cymbopogon nardus, Ocimum basilicum e Eucalyptus urograndis em ninfas do 3° e 4° estádios de desenvolvimento de Triatoma infestans por ação tópica e pressão a vapor (fumigação. Para o teste tópico foram realizadas duas repetições cada uma com cinco ninfas de 3° e 4° estádios de desenvolvimento de T. infestans. Foi aplicado 1µL de cada concentração na região dorsal de cada indivíduo. Para o teste de fumigação foram utilizados potes de 2 L com tampa vedante. Os óleos essenciais de O. gratissimum e E. urograndis apresentaram atividade inseticida para T. infestans, e A. humile e C. nardus apresentaram em média 5 e 15% de mortalidade, respectivamente. Para fumigação, somente o óleo de E. urograndis teve efeito, com 100% de mortalidade sobre T. infestans, sendo que para os outros óleos não foi observado efeito de fumigação. Esse trabalho relata pela primeira vez a ação inseticida de E. urograndis em potes fumígenos para o controle de triatomíneos. Os resultados são importantes devido à busca por novos modelos moleculares com ação inseticida em triatomíneos resistentes aos inseticidas sintéticos.Chagas disease is a major public health problem in Latin America. This work aimed to evaluate the insecticide action of essential oils of Anacardium humile, Cymbopogon nardus, Ocimum basilicum and Eucalyptus urograndis on Triatoma infestans nymphs of third and fourth stages of development by topic action and steam pressure (fumigation. Two repetitions were performed at the topic test with five nymphs of third and fourth stages of development of T. infestans. It was applied 1µL of each concentration on the dorsal region of each nymph. Two-liter pots with sealing covers were used at the fumigation test. The essential oils of O. gratissimum and E

  14. Relação entre Triatoma infestans, aves domésticas e o homem num povoado de Santiago del Estero, Argentina The relationship between Triatoma infestans, poultry, and humans in a rural settlement in Santiago del Estero, Argentina

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    Patrícia P. Gajate

    1994-03-01

    Full Text Available Determinou-se a associação entre Triatoma infestans, aves domésticas e o homem no povoado de Trinidad, Santiago del Estero, Argentina. Para coletar triatomíneos foram realizadas quatro amostragens no peridomicílio de seis casas, utilizando-se o método de captura hora/homem (dezembro de 1991 a outubro de 1992. Os anexos foram classificados em antrópicos (onde o homem realiza atividades cotidianas e não-antrópicos. Além disso, determinou-se o perfil alimentar dos barbeiros. De um total de 134 ecótopos investigados, 21% tinham T. infestans; 22% possuíam aves domésticas; e 54% eram antrópicos. Em 25% destes ecótopos foram encontradas aves e T. infestans simultaneamente. As aves foram os únicos animais domésticos associados com o T. infestans, sendo que esta relação só ocorreu nos ecótopos antrópicos. A proporção de ingestas em aves (61/146 foi altamente significativa. Em Trinidad não há galinheiros, utilizando-se, assim, os anexos entrópicos do peridomicílio como local para se construir os ninhos para as aves. tanto pelos elementos usados na preparação dos ninhos como pelo uso posterior dos mesmos, poder-se-ia estabelecer um fluxo periódico de barbeiros do intradomicílio para o peridomicílio, e vice-versa, através do transporte passivo. Esta relação estreita entre Triatoma infestans, aves domésticas e o homem, encontrada nos ecótopos antrópicos, onde também existem outros reservatórios de T. cruzi, como os cães, favorece a manutenção de colônias de barbeiros domiciliares e a transmissão da doença de Chagas ao homem.An association was determined between Triatona infestans, poultry, and humans in Trinidad, in the Province of Santiago del Estero, Argentina. To collect triatomines, four samples were taken at the area immediately surrounding six houses by the one hour/man capture method (December 1991 - October 1992. Peridomiciliary ecotopes were classified as arthropic (where humans carry out daily

  15. AO operations at Gemini South

    Science.gov (United States)

    Marin, Eduardo; Cardwell, Andrew; Pessev, Peter

    2014-08-01

    The 8m Gemini South telescope is entering an exciting new era of AO operations, which put it at the forefront of astronomical AO in terms of both wide field AO, and extreme-AO systems. Major milestones achieved were the successful commissioning of GeMS, in 2012, and GPI, in late 2013 and early 2014. Currently we are operating two of the worlds most advanced astronomical AO systems. Gemini, running primarily in queue, must balance the promise of AO with the demands of the community to use non-AO instruments. We discuss the current state of the two AO systems, and their operational models. The preparations that go into planning each AO run, the difficulties in scheduling around non-AO instruments, and the differences between scheduling LGS AO and non-LGS AO are discussed.

  16. Triatoma delpontei Romaña & Abalos, 1947 (Hemiptera, Triatominae) en el estado brasileño de "Rio Grande do Sul" Triatoma delpontei Romaña & Abalos, 1947 (Hemiptera, Triatominae) in Rio Grande do Sul State, Brasil

    OpenAIRE

    Roberto Salvatella Agrelo; Yester Basmadjian; Raquel Rosa; Andres Puime

    1993-01-01

    Triatoma delpontei Romaña y Abalos, 1947 es una especie de triatomineo silvestre, de marcada ornitofilia, que se asocia preferentemente al psitácido Myiopsitta monachus (Boddaert, 1783) colonizando sus nidos. Especie que se identifica con zonas de forestas subtropicales secas o xerofíticas propias de las provincias biogeográficas continentales chaqueñas, es notificada para Brasil en la microregión de la campaña de Río Grande do Sul (barra de Quaraí, Municipio de Uruguaiana).Triatoma delpontei...

  17. Detección de triatominos (Hemiptera: Reduviidae en ambientes domésticos y extradomésticos. Corrientes, Argentina Detection of triatomines (Hemiptera: Reduviidae in domiciliary and extra-domiciliary ecotopes. Corrientes, Argentina

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    Miryam Pieri Damborsky

    2001-08-01

    Full Text Available Esta investigación tiene como objetivo identificar los triatominos que predominan en ambientes domésticos y extradomésticos de diferentes departamentos de la Provincia de Corrientes mediante muestreos llevados a cabo desde 1985 hasta 1995. En los ecótopos domiciliarios se detectaron Triatoma infestans (Klug, 1834, Triatoma sordida (Stål, 1859, Panstrongylus geniculatus (Latreille, 1811 y Panstrongylus megistus (Burmeister, 1835. T. infestans y T. sordida se hallaron también en ambientes peridomésticos. Triatoma platensis (Neiva, 1913, Psammolestes coreodes (Bergroth, 1911, T. sordida y Triatoma rubrovaria (Blanchard, 1843 se colectaron en biótopos silvestres. En los domicilios, se confirmó el predominio de T. infestans. Esta especie fue la única parasitada por tripanosomas cruziformes. Si bien T. sordida fue más frecuente en los ecótopos extradomésticos, se demostró su tendencia a colonizar los ambientes domésticos.In order to identify intra- and extra-domiciliary triatomines, a study in different counties of Corrientes Province, Argentina, was conducted from 1985 to 1995. Triatoma infestans (Klug, 1834, Triatoma sordida (Stål, 1859, Panstrongylus geniculatus (Latreille, 1811, and Panstrongylus megistus (Burmeister, 1835 were detected in domestic ecotopes. T. infestans and T. sordida were also found in the peridomicile. Triatoma platensis (Neiva, 1913, Psammolestes coreodes (Bergroth, 1911, T. sordida, and Triatoma rubrovaria (Blanchard, 1843 were collected in sylvatic biotopes. T. infestans was confirmed as the predominant species in the domestic environment and was the only species infected with Trypanosoma cruzi-like parasites. T. sordida was most frequent in extra-domiciliary ecotopes, but could be considered capable of colonizing human dwellings.

  18. Infecção natural de Triatoma vitticeps (Stal, 1859 por flagelados morfologicamente semelhantes a Trypanosoma cruzi (Chagas, 1909 no Estado do Espírito Santo

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    Santos Claudiney Biral dos

    2006-01-01

    Full Text Available Espécimes adultos de Triatoma vitticeps são capturados freqüentemente por moradores em áreas rurais do Estado do Espírito Santo, Brasil. Com o objetivo de determinar o índice de infecção natural desta espécie, examinamos os dejetos de 116 espécimes silvestres, capturados em 27 municípios do estado, após repasto sanguíneo em ave e dejeção espontânea. Destes, 100 (86,2% estavam infectados por flagelados morfologicamente semelhantes a Trypanosoma cruzi. Detectamos índices de infecção natural de Tritoma vitticeps superiores ao de estudos anteriores. A baixa incidência da doença de Chagas no estado se deve provavelmente a dejeção tardia deste vetor visto que trabalhos sobre especificidade alimentar demonstraram presença marcante de Tritoma vitticeps no intradomicilio e contato freqüente com o homem. O elevado índice de infecção natural observado reforça a necessidade de se manter a vigilância entomológica sobre este triatomíneo.

  19. 华北地区杨树烂皮病发生发展气象适宜度预报模型%Forecasting Model of Meteorological Suitability for Occurrence and Development of Valsa sordida Nits in North China

    Institute of Scientific and Technical Information of China (English)

    王纯枝; 郭安红; 王玉玲; 周玉江; 毛留喜

    2011-01-01

    Based on the data of meteorology and monitoring on Valsa sordida Nits during 2002 - 2008 in 18 observation stations of Hebei province, the relationship between the occurrence and development of Valsa sordida Nits and meteorological conditions was analyzed applying correlation and regression analysis method. The results showed that the precipitation, rain day, relative humidity and temperature-humidity coefficient were positively related to the incidence of Valsa sordida Nits. It was favorable to the development of Valsa sordida Nits as the precipitation, rain day, relative humidity and temperature-humidity coefficient increased, while it was not favorable to the development of Valsa sordida Nits as the sunshine duration, extreme air temperature increased. The key meteorological factors which affected the occurrence and development of Valsa sordida Nits in Hebei province were temperature-humidity coefficients in March and April,the sunshine hours in the third ten days of March and April, air temperature in the first and third ten days of April, the extremely minimum air temperature in the first ten days of March and April and also last December respectively. The meteorological suitability forecasting model was established based on the normalized key meteorological factors. The validation results by the historical return test showed that forecasting accuracy for meteorological suitability models was 91% by comparing meteorological grade with actual grade. The meteorological grade of the occurrence fastigium during May to June in 2009 was forecasted with forecasting accuracy by 78% by trial forecast. The meteorological conditions in March and April were the key environmental factors affecting the occurrence and development of this disease in North China. The model could be put into the operational application in North China.%利用河北省18个代表站点2002-2008年杨树烂皮病病情资料和相应气象资料,通过相关、回归分析等方法,分析河

  20. AO Psc time keeping

    Science.gov (United States)

    Bonnardeau, Michel

    2015-07-01

    Eleven seasons, from 2004 to 2014, of photometric monitoring of the intermediate polar AO Psc are presented and are compared with previous observations. The spin up of the white dwarf is found to be slowing down. The amplitudes of the modulated and non-modulated components of the brightness are found to have undergone a major change in 2007.

  1. Fungal flora of the digestive tract of Triatoma infestans (Hemiptera: Reduviidae from Argentina Flora fúngica de tractos digestivos en Triatoma infestans (Hemiptera: Reduviidae en Argentina

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    G. A. Marti

    Full Text Available A survey of the fungal microbiota of the digestive tract of Triatoma infestans (Klug (Hemiptera: Reduviidae adults was carried out. Insects captured in the field from different provinces in Argentina, as well as individuals reared in artificial colonies, were used for dissection. Axenic cultures of the fungal species were identified and were deposited with mycological collections at La Plata , Argentina. A total of 33 fungal species, with the exception of three that were mycelia sterilia, belonging to 11 genera were identified. Thirty two species belonged to Ascomycota (Eurotiomycetes and Sordariomycetes and one to Zygomycota (Zygomycetes. The genera with the greatest number of species were Penicillium (15, Aspergillus (5, and Cladosporium (2. Among the isolated fungi, some of the species were entomopathogenic or pathogens of humans and other animals.En el presente estudio se realizó un relevamiento de la flora fúngica microbiana en tractos digestivos de adultos de Triatoma infestans (Klug (Hemiptera: Reduviidae. Se disecaron insectos capturados del campo en diferentes provincias Argentinas, así como también se utilizaron individuos de una colonia artificial. Fueron realizados cultivos axénicos de las especies fúngicas aisladas, los que fueron identificados y luego depositados en las colecciones de hongos entomopatógenos del CEPAVE La Plata , Argentina. Fueron identificadas 33 especies fúngicas perteneciente a 11 géneros. Treinta y dos especies pertenecen a Ascomycota (Eurotiomycetes y Sordariomycetes y una a Zygomycota (Zygomycetes. Los géneros con mayor número de especies fueron Penicillium (15, Aspergillus (5, y Cladosporium (2. Entre los aislamientos fúngicos, algunas de las especies encontradas son entomopatogénicas o patógenas de humanos y otros animales.

  2. Marking Triatoma brasiliensis, Triatoma pseudomaculata and Rhodnius nasutus Nymphs with Trace Elements: Element Persistence and Effects of Marking on Insect Mortality.

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    Carolina Valença-Barbosa

    2016-03-01

    Full Text Available Field ecologists often rely on mark-release-recapture (MRR experiments to estimate population dynamics parameters for a given species. In the case of a medically important taxon, i.e., a disease vector, inferences on species survival and dispersal rates are particularly important as they have the potential to provide insights into disease transmission dynamics in endemic areas. Medical entomologists have traditionally used fluorescent dusts to externally mark the cuticle of insects. However, dust marking is usually restricted to the adult life stage because immature insects lose the mark when they molt.We evaluated the efficacy of 13 trace elements in marking nymphs of three native Brazilian Chagas disease vectors: Triatoma brasiliensis, Triatoma pseudomaculata, and Rhodnius nasutus. Cr and Cu were detected in over 97% of T. brasiliensis (34/35 31/31 for Cr and Cu, while Cu and Mn were detected in more than 95% of T. pseudomaculata (29/29 for Cu and 28/29 for Mn tested 120 days after marking. Only Mn marked over 90% of R. nasutus nymphs (38/41. Overall, trace elements had no negative effects on T. pseudomaculata longevity, but As-marked T. brasiliensis nymphs (p<0.01, and Cd-marked R. nasutus nymphs (p<0.01 had significantly shorter lifespan.Previous evidence shows that there is little or no genetic differentiation between populations at the microgeographic level, which often precludes indirect estimations of dispersal capability based on genetic markers. In such situations, MRR studies are more suitable as they measure insect movement directly from one site to another, instead of effective migration (i.e. gene flow. The determination of a reliable and persistent marking method is the first step towards the development of meaningful ecological estimates through the application of MRR methodology. Here, we have identified trace elements that can be used for mark and recapture studies of three triatomine species in Brazil.

  3. The central projection of cephalic mechanosensory axons in the haematophagous bug Triatoma infestans

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    Insausti Teresita C

    2000-01-01

    Full Text Available The projections of mechanosensory hairs located on the dorsal and lateral head of the adult haematophagous bug Triatoma infestans were analyzed by means of cobalt filling. Axons run into the anterior and posterior tegumentary nerve and project through the brain to the ventral nerve cord. The fibres are small in diameter and run as a fascicle. Some branches run into suboesophageal and prothoracic centres; others run as far as to the mesothoracic ganglion. These sensory projections resemble that of wind-sensitive head hairs of the locust. The functional role of this sensory system in this species is discussed.

  4. Existence of a sex pheromone in Triatoma infestans (Hemiptera: Reduviidae: I. Behavioural evidence

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    Gabriel Manrique

    1995-10-01

    Full Text Available Assembling behaviour associated with mating was investigated in Triatoma infestans. The spatial distribution of both sexes was observed by video films, in the presence or absence of a copulating pair. Males aggregated around copulating pairs. Females did not exhibit this behaviour and their mean spatial density remained unaffected. Spontaneous aggregation tendency was observed in males in the absence of a copulating pair, but the temporal course significantly differed from that observed in the presence of a mating pair. Results support the existence of an aggregation signal that is released during mating, affecting the behaviour of males.

  5. Fauna triatominae do estado da Bahia, Brasil: IV - Triatoma Melanocephala Neiva & Pinto, 1923

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    Ítalo A. Sherlock

    1980-12-01

    Full Text Available Triatoma melanocephala - Neiva & Pinto, 1923, espécie rara e de distribuição geográfica restrita, é redescrita no presente trabalho. Exemplares adultos foram coletados em domicílio e adultos e ninfas foram pela primeira vez encontrados em gravatás no ambiente silvestre, infectados pelo Trypanosoma cruzi. A espécie foi criada em laboratório com dificuldades, gastando cerca de 350 dias no mínimo para o desenvolvimento de ovo a adulto; mostrando-se bem susceptível á infecção experimental pelo T. cruzi.Triatoma melanocephala Neiva & Pinto, 1923, a rare species of triatomine with a restricted geographic distribution, is redescribed in the present paper. Specimens of adults were collected inside houses and adults and nymphs naturally infected with T. cruzi were found in bromeliad epiphyts in sylvatic sites. This species of bug was reared with difficulty in the laboratory and required about 350 days to develop from egg to adult.

  6. Morphometric analysis of Triatoma dimidiata populations (Reduviidae:Triatominae from Mexico and Northern Guatemala

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    P Lehmann

    2005-08-01

    Full Text Available Triatoma dimidiata is one of the major vectors of Chagas disease in Latin America. Its range includes Mexico, all countries of Central America, Colombia, and Ecuador. In light of recent genetic analysis suggesting that the possible origin of this species is the Yucatan peninsula, we have analyzed populations from the state of Yucatan, San Luis Potosi, and Veracruz in Mexico, and a population from the southern region of the Yucatan peninsula located in Northern Guatemala, the region of El Peten. Classical morphometry including principal component, discriminant, sexual dimorphism, and wing asymmetry was analyzed. San Luis Potosi and Veracruz populations were indistinguishable while clearly separate from Yucatan and Peten populations. Despite important genetic differences, Yucatan and Peten populations were highly similar. Yucatan specimens were the smallest in size, while females were larger than males in all populations. Only head characters were necessary to distinguish population level differences, although wing fluctuating asymmetry was present in all populations. These results are discussed in light of recent findings suggesting genetic polymorphism in most populations of Triatoma dimidiata south of Chiapas to Ecuador.

  7. Influence of Triatoma dimidiata in Modulating the Virulence of Trypanosoma cruzi Mexican Strains

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    E. Guzman-Marin

    2012-01-01

    Full Text Available The epidemiology of Chagas disease is complex. There are different vectors and reservoirs and different clinical manifestations. In order to assess whether the biological behavior of three strains isolated in southeastern Mexico (H4 isolated from human, Z17 isolated from Didelphis sp., and V isolated from T. dimidiata could be modified during passage through the vector T. dimidiata, the parasitemia curve, the amount of amastigote nests, and mortality of BALB/c infected with blood trypomastigotes of T. cruzi were evaluated. Strains were maintained in continuous passage from mouse to mouse and in animals infected with metacyclic trypomastigotes. The parasitemia curves were significantly different ( between mice to mice and triatoma to mice groups in strains H4 and Z17, and was also observed fewer amastigote nests in cardiac tissue ( strain H4 with higher number versus all groups and Z17 between mice to mice and triatoma to mice 45 days after inoculation. It is concluded that T. dimidiata influences in modulating the virulence of strains of T. cruzi in the region. Further studies of the intestinal tract of the insect in search for some protein molecules involved in regulating may clarify the virulence of the parasite.

  8. AO Group Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Olivier, S

    2005-10-04

    The Adaptive Optics (AO) Group in I Division develops and tests a broad range of advanced wavefront control technologies. Current applications focus on: Remote sensing, High power lasers, Astronomy, and Human vision. In the area of remote sensing, the AO Group leads a collaborative effort with LLNL's Nonproliferation, Arms Control & International Security (NAI) Directorate on Enhanced Surveillance Imaging. The ability to detect and identify individual people or vehicles from long-range is an important requirement for proliferation detection and homeland security. High-resolution imaging along horizontal paths through the atmosphere is limited by turbulence, which blurs and distorts the image. For ranges over {approx}one km, visible image resolution can be reduced by over an order of magnitude. We have developed an approach based on speckle imaging that can correct the turbulence-induced blurring and provide high resolution imagery. The system records a series of short exposure images which freeze the atmospheric effects. We can then estimate the image magnitude and phase using a bispectral estimation algorithm which cancels the atmospheric effects while maintaining object information at the diffraction limit of the imaging system.

  9. Carta ao Leitor

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    Equipe Editorial RMCEAD

    2015-06-01

    Full Text Available Aos Leitores Caríssimos leitores, Com grande entusiasmo, apresentamos a mais nova edição da Revista Multitexto do CEAD/Unimontes. Esta edição conta com 12 novos trabalhos que, sob um prisma transdisciplinar de reflexões, tecem suas leituras e arranjos metodológicos incidindo sobre as ciências e a realidade, seu teor e proposta. Trata-se de artigos originais e de revisão que promovem um diálogo aberto com o mundo contemporâneo. O estado da arte de diversas linhas temáticas foram selecionados para que a comunidade científica possa celebrar o conhecimento dispensado nas páginas materiais e virtuais desse periódico em crescimento. Em seu terceiro ano de experiências em coligir e abrigar estudos de recorte interdisciplinar, com enfoque especial ao rol de temáticas da Educação a Distância, a Revista Multitexto adquire mais entusiasmo. Entusiasmo para se debruçar sobre textos que se expandem sobre o cotidiano e integram os caminhos acadêmicos aos caminhos da vida. Você será capaz de imergir em pesquisas e perspectivas que celebram o conhecimento como ferramenta de transformação da realidade. E, como costumeiro deste periódico, equipe editorial e instituição, trata-se da celebração de uma paixão inveterada pelo conhecimento. A jornada deste periódico caminha para a maturação de seus autos e de motivações originárias. É cada vez mais seguro o ensejo pela pesquisa nesta instituição de salvaguarda e é cada vez mais expressivo o esforço conjunto para que o conhecimento através de múltiplos textos – como propõe a denominação deste periódico – transpasse a realidade com frutos e benefícios amplos. Aprecie as colaborações da presente edição e junte-se neste tráfego do conhecimento, no qual o mundo hoje se move. Com grande estima, Equipe Editorial

  10. Hallazgo de Triatoma platensis Neiva, 1913 (Hemiptera, Triatominae en el estado brasileño de "Rio Grande do Sul" Finding of Triatoma platensis Neiva, 1913 (Hemiptera, Triatominae in the Brazilian State of "Rio Grande do Sul"

    Directory of Open Access Journals (Sweden)

    Roberto Salvatella

    1991-02-01

    Full Text Available Triatoma platensis Neiva 1913 (Hemiptera, Triatominae, especie ornitófila, con área de dispersión conocida en Argentina, Bolivia, Paraguay y Uruguay es notificada en un primer registro para Brasil. Hallada en el municipio de Uruguaiana (Río Grande do Sul en nidos de Anumbius annumbi (Vieillot, 1817 (Passeriformes, Furnariidae donde cohabitaba con cricétidos de la especie Orizomys flavescens. Ningún ejemplar de los siete colectados se presentó infectado por Trypanosoma cruzi.Triatoma platensis, an ornitophilic species, found in Argentina, Bolivia, Paraguay and Uruguay is reported for the first time in Brazil. It was found in the county of Uruguaiana (Rio Grande do Sul in nests of Anumbius annumbi (Vieillot, 1817 (Passeriformes, Furnariidae where it cohabited with cricetids belonging to the species Orizomys flavescens. None of the seven individuals collected were infected by Trypanosoma cruzi.

  11. Ecological control of Triatoma dimidiata (Latreille, 1811: five years after a Costa Rican pilot project

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    Rodrigo Zeledó

    2008-09-01

    Full Text Available An ecological pilot project for the control of Triatoma dimidiata allowed a new evaluation four and five years after environmental modifications in the peridomestic areas of 20 households. It was verified that the two groups of houses, 10 case-houses and 10 control-houses, were free of insects after those periods of time. In the first group, the owners started a chicken coop in the backyard and a colony of bugs was found there without infesting the house. In the second group, the inhabitants of one house once again facilitated the conditions for the bugs to thrive in the same store room, reaffirming that man-made ecotopes facilitates colonization. This ecological control method was revealed to be reliable and sustainable and it is recommended to be applied to those situations where the vectors of Chagas disease can colonize houses and are frequent in wild ecotopes.

  12. Extensive diversity of Trypanosoma cruzi discrete typing units circulating in Triatoma dimidiata from central Veracruz, Mexico.

    Science.gov (United States)

    Ramos-Ligonio, Angel; Torres-Montero, Jesús; López-Monteon, Aracely; Dumonteil, Eric

    2012-10-01

    Chagas disease (or American trypanosomiasis) is a parasitic disease of major public health importance, caused by Trypanosoma cruzi, which presents extensive genetic diversity. The parasite has been classified into six lineages or discrete typing units (TcI to TcVI) and we performed here the molecular characterization of the strains present in Triatoma dimidiata, the main vector in central Veracruz, Mexico. Unexpectedly, TcI only represented 9/33 strains identified (27%), and we reported for the first time the presence of TcII, TcIII, TcIV and TcV strains in Mexico, at a relatively high frequency (13-27% each). Our observations indicate a much greater diversity of T. cruzi DTUs than previously estimated in at least part of Mexico. These results have important implications for the understanding of the phylogeography of T. cruzi DTUs and the epidemiology of Chagas disease in North America.

  13. House infestation dynamics and feeding sources of Triatoma dimidiata in central Veracruz, Mexico.

    Science.gov (United States)

    Torres-Montero, Jesús; López-Monteon, Aracely; Dumonteil, Eric; Ramos-Ligonio, Angel

    2012-04-01

    Chagas disease is endemic in the state of Veracruz, Mexico, and we investigated here the dynamics of house infestation by Chagas disease vectors to understand disease transmission and design effective control interventions. Bug collections in 42 rural villages confirmed the widespread distribution of Triatoma dimidiata in central Veracruz. Unexpectedly, collection data further indicated a clear pattern of seasonal infestation by mostly adult bugs. Analysis of feeding sources with a polymerase chain reaction-heteroduplex assay indicated a frequent feeding on humans, in agreement with the high seroprevalence previously observed. Feeding sources also confirmed a significant dispersal of bugs between habitats. High dispersal capabilities and seasonal infestation may thus be a shared characteristic of several of the T. dimidiata sibling species from this complex. It would thus be critical to adapt vector control interventions to this behavior to improve their efficacy and sustainability, as the control of T. dimidiata has been notoriously challenging.

  14. Ecology of Triatoma rubrovaria (Hemiptera, Triatominae) in wild and peridomestic environments of Uruguay.

    Science.gov (United States)

    Salvatella, R; Rosa, R; Basmadjian, Y; Puime, A; Calegari, L; Guerrero, J; Martinez, M; Mendaro, G; Briano, D; Montero, C

    1995-01-01

    This paper describes population structure, spatial distribution and habitat selection of wild and peridomestic populations of Triatoma rubrovaria (Blanchard, 1843). Field studies were carried out at Las Piedras and La Bolsa in the Northern Department of Artigas, Uruguay. A semicircular sampling area, divided in seven or eight triangular sectors was sequentially examined from October 1990 to November 1991. At Las Piedras (typical wild habitat) 1063 T. rubrovaria bugs were collected from 84% of the rocky outcroops ("pedregales"). Abundance varied by season peaking in October-November (spring). Throughout the year, most of the population was made up of third, fourth and fifth instar nymphs; adults were found from October to March. In the peridomestic environment of La Bolsa, however T. rubrovaria was less common and showed a more irregular instar distribution. Colonized ecotopes, were those close to houses. In both sites T. rubrovaria was mainly associated with the geckonid Homonota uruguayensis and the cockroach Blaptica dubia.

  15. Ecology of Triatoma rubrovaria (Hemiptera, Triatominae in wild and peridomestic environments of Uruguay

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    Roberto Salvatella

    1995-06-01

    Full Text Available This paper describes population structure, spatial distribution and habitat selection of wild and peridomestic populations of Triatoma rubrovaria (Blanchard, 1843. Field studies were carried out at Las Piedras and La Bolsa in the Northern Department of Artigas, Uruguay. A semicircular sampling area, divided in seven or eight triangular sectors was sequentially examined from October 1990 to November 1991. At Las Piedras (typical wild habitat 1063 T. rubrovaria bugs were collected from 84 of the rocky outcroops ("pedregales". Abundance varied by season peaking in October-November (spring. Throughout the year, most of the population was made up of third, fourth and fifth instar nymphs; adults were found from October to March. In the peridomestic environment of La Bolsa, however T. rubrovaria was less common and showed a more irregular instar distribution. Colonized ecotopes, were those close to houses. In both sites T. rubrovaria was mainly associated with the geckonid Homonota uruguayensis and the cockroach Blaptica dubia.

  16. Immunohistochemical detection of a very high density lipoprotein (VHDL) in ovarian follicles of Triatoma infestans.

    Science.gov (United States)

    González, M S; Ronderos, J R; Rimoldi, O J; Brenner, R R

    2001-04-01

    The ability of Triatoma infestans ovarian follicles to synthesize a very high-density lipoprotein (VHDL) has been examined by immunohistochemical methods. This kind of lipoprotein can be envisaged as a storage hexameric protein present in the hemolymph of some insect species. VHDL immunoreactivity is observed in oocytes at different stages of maturation. The antigen is present in the oocyte cytoplasm as well as in the follicular epithelial cells. The immunopositive reaction in the apical surface of follicle cells suggests both a VHDL synthesis and a secretion process. Furthermore, VHDL seems to be stored into oocyte in yolk granules. On the contrary, no immunopositive reaction is observed in the intracellular spaces between follicle cells, suggesting that VHDL is not incorporated from hemolymph into the oocyte.

  17. Response of Triatoma infestans to pour-on cypermethrin applied to chickens under laboratory conditions

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    Ivana Amelotti

    2009-05-01

    Full Text Available This article reports the effects of a pour-on formulation of cypermethrin (6% active ingredient applied to chickens exposed to Triatoma infestans, the main vector of Chagas disease in rural houses of the Gran Chaco Region of South America. This study was designed as a completely random experiment with three experimental groups and five replicates. Third instar nymphs were fed on chickens treated with 0, 1 and 2 cc of the formulation. Nymphs were allowed to feed on the chickens at different time intervals after the insecticide application. Third-instar nymphs fed on treated chickens showed a higher mortality, took less blood during feeding and had a lower moulting rate. The mortality rate was highest seven days after the insecticide solution application and blood intake was affected until 30 days after the application of the solution.

  18. Circadian entrainment by light and host in the Chagas disease vector, Triatoma infestans.

    Science.gov (United States)

    Valentinuzzi, Verónica Sandra; Amelotti, Ivana; Gorla, David Eladio; Catalá, Silvia Susana; Ralph, Martin Roland

    2014-03-01

    Triatoma infestans (Reduviidae: Triatominae, "kissing bug") is the main insect vector of Trypanosoma cruzi, the causative agent of Chagas disease, a chronic trypanosomiasis infecting 10 million people world-wide. This hematophagous bug feeds on diurnal and nocturnal species during each host's quiescent time. As the hosts are also its major predators, kissing bugs are subjected to dual selective pressures from a single source. Therefore, synchronization of feeding with the host's behavior is critical to the insects' survival. We show that nonphotic signals linked to the host eclipse the role of light and dark as the primary circadian zeitgeber for these bugs, although light still strongly inhibits locomotor behavior directly. In nature, this combination provides the insect with great flexibility in organizing physiology and behavior: anticipating a quiescent host or avoiding its potential predation while remaining directly responsive to immediate environmental conditions. Manipulation of nonphotic entrainment could be a useful chronobiotic tool in the control of Chagas disease.

  19. Evidence of colonization of man-made ecotopes by Triatoma dimidiata (Latreille, 1811 in Costa Rica

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    Rodrigo Zeledón

    2001-07-01

    Full Text Available Triatoma dimidiata adults have been frequently found, during the last five years, in a dog kennel and a chicken coop, in the back yard of a well-built house, 15 km from San José, the capital of Costa Rica. In the chicken coop nymphs were also found. Two of the 11 dogs from the kennel were serologically positive for Trypanosoma cruzi infection. The inhabitants of the house, three adults and two children, were negative. This type of colonization by the insect, which is attracted to lights, is becoming common in old and new settlements, with different degrees of success, a fact with epidemiological implications and great relevance in the control strategies that can be applied.

  20. Incoordination, Paralysis and Recovery after Pyrethroid Treatment on Nymphs III of Triatoma infestans (Hemiptera: Reduviidae

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    Raúl A Alzogaray

    1997-05-01

    Full Text Available Symptoms of poisoning for deltamethrin and cis-permethrin on nymphs III of Triatoma infestans were described. The time required for incoordination and paralysis were determined. Deltamethrin was equal or more rapid in the onset of the first effect (accordingly to dose, and cis-permethrin in the onset of the second one. There were no significant differences between incoordination doses 50% (IncD50s at different times for the two pyrethroids. They showed equivalent incoordination power, but the nymphs treated with deltamethrin recovered slightly more rapid and in greater amount than the nymphs treated with cis-permethrin. The recovery was inhibited by the simultaneus application of piperonyl butoxide. This result suggests that biotransformation by mixed-function microsomal oxidases are involved in the process of recovery

  1. Developmental and reproductive patterns of Triatoma brasiliensis infected with Trypanosoma cruzi under laboratory conditions

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    Tiago G Oliveira

    2010-12-01

    Full Text Available The aim of this work was to study the interaction between Trypanosoma cruzi-1 and Triatoma brasiliensis. A group of 1st instar nymphs was initially fed on T. cruzi-infected mice and a control group was fed on uninfected mice. From the second feeding onwards, both groups were otherwise fed on non-infected mice. The resulting adults were grouped in pairs: infected male/uninfected female, uninfected male/infected female, infected male and female and uninfected male/uninfected female. The infection affected only the 1st instar nymphs, which took significantly more time to reach the 2nd instar than uninfected nymphs. The differences in the molting time between the infected and uninfected nymphs from the 2nd to the 5th instars were not statistically significant. Both groups presented similar rates of nymphal mortality and reproductive performance was not significantly affected by infection in any of the treatments.

  2. Environmental Determinants of the Distribution of Chagas Disease Vector Triatoma dimidiata in Colombia.

    Science.gov (United States)

    Parra-Henao, Gabriel; Quirós-Gómez, Oscar; Jaramillo-O, Nicolas; Cardona, Ángela Segura

    2016-04-01

    Triatoma dimidiata (Hemiptera: Reduviidae) is a secondary vector of Trypanosoma cruzi in Colombia and represents an important epidemiological risk mainly in the central and oriental regions of the country where it occupies sylvatic, peridomestic, and intradomestic ecotopes, and because of this complex distribution, its distribution and abundance could be conditioned by environmental factors. In this work, we explored the relationship between T. dimidiata distribution and environmental factors in the northwest, northeast, and central zones of Colombia and developed predictive models of infestation in the country. The associations between the presence ofT. dimidiata and environmental variables were studied using logistic regression models and ecological niche modeling for a sample of villages in Colombia. The analysis was based on the information collected in field about the presence ofT. dimidiata and the environmental data for each village extracted from remote sensing images. The presence of Triatoma dimidiata(Latreille, 1811) was found to be significantly associated with the maximum vegetation index, minimum land surface temperature (LST), and the digital elevation for the statistical model. Temperature seasonality, annual precipitation, and vegetation index were the variables that most influenced the ecological niche model ofT. dimidiata distribution. The logistic regression model showed a good fit and predicted suitable habitats in the Andean and Caribbean regions, which agrees with the known distribution of the species, but predicted suitable habitats in the Pacific and Orinoco regions proposing new areas of research. Improved models to predict suitable habitats forT. dimidiata hold promise for spatial targeting of integrated vector management.

  3. Domestic, peridomestic and wild hosts in the transmission of Trypanosoma cruzi in the Caatinga area colonised by Triatoma brasiliensis

    OpenAIRE

    Claudia Mendonça de Bezerra; Luciano Pamplona de Góes Cavalcanti; Rita de Cássia Moreira de Souza; Silvia Ermelinda Barbosa; Samanta Cristina das Chagas Xavier; Ana Maria Jansen; Relrison Dias Ramalho; Liléia Diotaiut

    2014-01-01

    The role played by different mammal species in the maintenance of Trypanosoma cruzi is not constant and varies in time and place. This study aimed to characterise the importance of domestic, wild and peridomestic hosts in the transmission of T. cruzi in Tauá, state of Ceará, Caatinga area, Brazil, with an emphasis on those environments colonised by Triatoma brasiliensis. Direct parasitological examinations were performed on insects and mammals, serologic tests were performed on household and ...

  4. O combate ao escorpionismo

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    Octavio de Magalhães

    1946-09-01

    Full Text Available O outor estuda os processos de combate aos acidentes pelas picadas dos escorpiões, visando: a Profilaxia e b Terapêutica. Refere-se à luta nos campos e nas cidades e aqui, dentro e fora dos domicílios. Assinala os diferentes métodos para o combate a êsses artrópodos peçonhentos. Fala na luta direta, indireta, na vacinação pelo anaveneno, na propaganda pela educação racional da população contra o perigo dos acidentes. Refere-se à "cata" dos escorpiões, à luta química, ao emprêgo de animais escorpiófagos, á feitura de casas e jardins anti-escorpiões. Na primeira parte do presente trabalho o autor trata da luta química pelo d. D. T. contra os tityus bahiensis e serrulatus, concluindo que êste corpo químico é um poderoso elemento de luta contra êstes escorpiões. Na segunda parte do trabalho, o autor trata da terapêutica dos acidentes. Mostra a necessidade do emprêgo convenientemente da única terapêutica racional e eficaz contra a intoxicação escorpiônica: a soroterapia específica. Assinala a necessidade da injeção de doses maciças de um sôro de alta valência, preparado em bovídeos, para evitar o mais possível o choque anafilático, no menor tempo possível após as picadas. Aconselha o empêgo de anaveneno escorpiônico, para vacinação principalmente de crianças de baixa idade, nos lugares fortemente infestados pelos escorpiões (principalmente Tityus serrulatus, maximé quando no local não houver sôro anti-escorpiônico específico, como meio preventivo contra a gravidade das intoxicações. Cita finalmente os trabalhos recentes de Grasset, Shaasfsma e Hodgson, na África do Sul, que confirmam muitas idéias do autor e mostram a unidade universal do síndromo escorpiônico descrito no Brasil.

  5. Hábito alimentar de Triatoma vitticeps no Estado do Rio de Janeiro, Brasil Feeding patterns of Triatoma vitticeps in the State of Rio de Janeiro, Brazil

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    Teresa Cristina M Gonçalves

    2000-08-01

    Full Text Available OBJETIVO: O estudo do hábito alimentar dos triatomíneos tem contribuído para o conhecimento da sua biologia no habitat natural. Triatoma vitticeps, espécie que vem invadindo freqüentemente o domicílio apresentando-se infectado por T. cruzi, foi analisado sob esse aspecto, possibilitando conhecer a situação epidemiológica da área. MÉTODOS: De fevereiro de 1989 a abril de 1993, 122 espécimes de T. vitticeps foram capturados em duas áreas da localidade de Triunfo, 2° Distrito do Município de Santa Maria Madalena (RJ. Os insetos foram dissecados para a retirada do conteúdo estomacal. Os anti-soros utilizados foram: homem, vaca, cavalo, cão, porco, tatu, gambá, roedor e ave. RESULTADOS: Do total analisado, 79 estavam positivos e 43 negativos para os anti-soros testados: tatu (30,3% > homem e porco (13,1% > ave e cão (11,5% > cavalo (5,7% > gambá (4,9% > roedor (4,1% > boi (3,3%. As fontes alimentares identificadas variaram de 1 a 4 e 6: 0 - 25,41%; 1 - 45,08%; 2 - 10,66%; 3 - 6,56%; 4 - 1,64% e 6 - 0,82%. Quanto à infecção por T. cruzi, 74 espécimes (65,54% estavam positivos, 39 (34,51% negativos e 9 não foram examinados. CONCLUSÕES: Os resultados caracterizam o hábito silvestre de T. vitticeps e a tripanosomíase como uma enzootia. A vigilância epidemiológica se faz necessária para o acompanhamento do comportamento dessa espécie.OBJECTIVE: Feeding patterns of triatomines have contributed to elucidate its biology. Triatoma vitticeps, naturally infected with T. cruzi, has been found in domiciles. Its behavior and epidemiological patterns were investigated. METHODS: One-hundred and twenty two specimens of T. vitticeps were captured from February 1989 to April 1993 in two areas of Triunfo municipality, a subdistrict of Santa Maria Madalena municipal district, State of Rio de Janeiro, Brazil. The insects were dissected and their intestinal contents were removed and tested. It was used antisera from: man, cow, horse, dog

  6. Influência de hábitos antrópicos na dispersão de Triatoma pseudomaculata Corrêa & Espínola, 1964, através de Mimosa tenuiflora (Willdenow (Mimosaceae no Estado do Ceará, Brasil

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    Freitas Simone Patrícia Carneiro

    2004-01-01

    Full Text Available Triatoma pseudomaculata foram capturados em entrecascas de Mimosa tenuiflora (jurema-preta em ecótopo silvestre de sete municípios do Estado do Ceará. Esta planta apresenta alta taxa de cobertura geográfica nas áreas de caatingas, sendo freqüentemente utilizada pela população rural. A presença de triatomíneos no peridomicílio, na região do Cariri, pode estar associada ao uso pela população da madeira de M. tenuiflora, quando trazida do ambiente silvestre, repleta de triatomíneos, facilitando conseqüentemente sua mobilização e dispersão.

  7. Triatoma brasiliensis (Neiva,1911 (Hemiptera, Reduviidae: observações sobre seu comportamento em relação à fonte alimentar em galinheiro experimental Triatoma brasiliensis Neiva, 1911 (Hemiptera, Reduviidae: observations on dynamics of feeding behavior in an artificial ecotope

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    Vera Lúcia C. C. Rodrigues

    1995-06-01

    Full Text Available O presente trabalho tem a finalidade de estudar o comportamento de triatomíneos no interior de ecótopos artificiais. Para tanto, construiu-se galinheiro experimental com paredes internas formadas por blocos de barro desmontáveis, abrigando aves como fonte alimentar. O galinheiro foi subdividido em 4 áreas, duas destas separadas por telas e tijolos furados, sendo a menor o local de soltura dos triatomíneos e a maior o galinheiro propriamente dito. Dessa maneira buscou-se a coleta de informaç��es a respeito do deslocamento, sobrevida e locais de abrigo de ninfas do 4º estádio de Triatoma brasiliensis, na certeza de que os mesmos iriam se acomodar nas proximidades da fonte alimentar. As paredes do galinheiro foram desmontadas e pesquisadas mensalmente durante 6 meses e as ninfas encontradas, marcadas com diferentes cores de acordo com o mês da leitura e a posição das mesmas nas áreas. Os resultados alcançados mostraram que 88,5% das ninfas estavam engorgitadas e foram encontradas próximas ao local de repouso (poleiro da fonte alimentar. Após a primeira alimentação, não abandonavam as proximidades da fonte. Os resultados alcançados apresentam, não apenas interesse epidemiológico, como também interesse na área de controle dos vetores da doença de Chagas, tendo em vista as possíveis implicações quanto aos procedimentos na aplicação do inseticida.In order to study the behavior of triatomine bugs under artificial ecotopic conditions, an experimental, double-walled poultry coop was built. Its inside walls were made of sun-dried clay blocks which could be broken apart for better access to the insects hiding in their crevices. The coop was subdivided into two main areas of different sizes using wire netting and a wall made of perforated bricks. The chickens, the only food source available for the triatomines, were kept in the larger, rectangular area, further subdivided diagonally. The triatomines were released in the

  8. Biological cycle and preliminary data on vectorial competence of Triatoma boliviana in laboratory conditions.

    Science.gov (United States)

    Durán, Pamela; Siñani, Edda; Depickère, Stéphanie

    2014-12-01

    With more than 140 potential vectors of Chagas disease, it is important to better know the biology and especially the vectorial capacity of the triatomine species which live in the surroundings of human dwellings. In Bolivia where 17 triatomine species are reported, the principal vector is Triatoma infestans. In some valleys of the department of La Paz where T. infestans is not present, a new species (Triatoma boliviana) was described in 2007. This species lives in a sylvatic environment not far away from the dwellings, and occasionally some individuals are found inside the houses. This study was carried out to describe the biological cycle of T. boliviana and to determine its vectorial competence. The development of a cohort of 95 nymphs of first instar (N1) was followed through nymphal instars and adult stage until death in laboratory (22°C). They were fed twice a week on an immobilized mouse. The median egg-to-adult development time was 8.4 months. The mortality by nymphal instar was lower than 7% except for N1 (67%) and N5 (18%). All nymph instars needed at least two feedings to molt (until six feedings for N5). The differentiation of a nymph into a female or a male could not be detected until the fifth instar for which the food intake was greater for a nymph developing into a female. Adults fed about once a week. The adult life span was around 400 days. The fecundity was 4.2 eggs/female/week, with a hatching rate of 50% and a hatching time of 39 days. In the same conditions, T. infestans showed a similar fecundity but a greater hatching rate and hatching time. A trial for rearing the adults at a higher temperature (26°C) showed a drastic fall in the fecundity and in the hatching rate. The vectorial competence was analyzed for fifth instars and adults by three parameters: the ability to feed on human beings, the capacity to be infected by T. cruzi and the postfeeding defecation delay. Results showed a relatively high vectorial competence: (1) insects fed

  9. Geographic Distribution and Ecology of Triatoma dimidiata (Hemiptera: Reduviidae) in Colombia.

    Science.gov (United States)

    Parra-Henao, Gabriel; Angulo, Víctor Manuel; Osorio, Lisardo; Jaramillo-O, Nicolás

    2016-01-01

    Triatoma dimidiata Latreille is the second most important vector of Chagas' disease in Colombia and is found in urban and periurban areas. From January 2007 to June 2008, we performed field work in 8 departments, 18 municipalities, and 44 rural villages, covering most of its known distribution and all of its ecological zones in the country. The goal was to determine the geographical distribution, the ecology, and house infestation indices of T. dimidiata over its range and hence the Chagas' disease transmission risk. In Colombia, T. dimidiata occupies a wide variety of ecosystems, from transformed ecosystems in the Andean biome with shrub and xerofitic vegetation to very dense forests in the humid tropical forests in the Sierra Nevada of Santa Marta. According to genetic and ecological criteria, at least two T. dimidiata forms of this species are present: populations from the northwest of the country (Caribbean plains) are restricted to palm tree habitats, and domestic involvement is limited to sporadic visits because of attraction by light; and populations of the east region (Andean mountains) presenting a complex distributional pattern including sylvatic, peridomestic, and domiciliated ecotopes, and occupying a great variety of life zones. The latter population is of epidemiological importance due to the demonstrated migration and genetical flow of individuals among the different habitats. Control, therefore, must take into account its diversity of habitats.

  10. Genetic Variability and Microdistribution of Triatoma infestans Genotypes and Trypanosoma cruzi Clones in Arequipa Region (Peru

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    Brenière Simone F

    1997-01-01

    Full Text Available The genetic variability of Triatoma infestans and Trypanosoma cruzi populations was studied by isoenzyme analysis in two distinct areas of Arequipa province (Peru; one, Santa Rita de Siguas, being an endemic area for Chagas' disease, the second, Arequipa, recently infected. Analysis of T. infestans genetic variability indicates, (i temporal stability of genotypes found in Santa Rita de Siguas, (ii high genetic differences between Arequipa and Santa Rita de Siguas populations suggesting minor contact between them, (iii multiple origin of the T. infestans population in Arequipa, and (iv poor dispersal capacity of T. infestans: the panmictic unit could be reduce to a house. Parasite isoenzyme analysis was performed in 29 Peruvian stocks of T. cruzi, mainly isolated from bugs taken in a single locality, Santa Rita de Siguas. The results show, (i a high genetic polymorphism, (ii nine different multilocus genotypes were detected and clustered in two different clades, (iii most of the parasite isolates pertained to one of the clade and were genetically similar to those analyzed 12 years before. This sample allowed the study of the mating system of T. cruzi in strict sympatic conditions and gave more strength to the hypothesis of the clonal structure of T. cruzi populations

  11. Residual infestation and recolonization during urban Triatoma infestans Bug Control Campaign, Peru.

    Science.gov (United States)

    Barbu, Corentin M; Buttenheim, Alison M; Pumahuanca, Maria-Luz Hancco; Calderón, Javier E Quintanilla; Salazar, Renzo; Carrión, Malwina; Rospigliossi, Andy Catacora; Chavez, Fernando S Malaga; Alvarez, Karina Oppe; Cornejo del Carpio, Juan; Náquira, César; Levy, Michael Z

    2014-12-01

    Chagas disease vector control campaigns are being conducted in Latin America, but little is known about medium-term or long-term effectiveness of these efforts, especially in urban areas. After analyzing entomologic data for 56,491 households during the treatment phase of a Triatoma infestans bug control campaign in Arequipa, Peru, during 2003-2011, we estimated that 97.1% of residual infestations are attributable to untreated households. Multivariate models for the surveillance phase of the campaign obtained during 2009-2012 confirm that nonparticipation in the initial treatment phase is a major risk factor (odds ratio [OR] 21.5, 95% CI 3.35-138). Infestation during surveillance also increased over time (OR 1.55, 95% CI 1.15-2.09 per year). In addition, we observed a negative interaction between nonparticipation and time (OR 0.73, 95% CI 0.53-0.99), suggesting that recolonization by vectors progressively dilutes risk associated with nonparticipation. Although the treatment phase was effective, recolonization in untreated households threatens the long-term success of vector control.

  12. Population structure of the Chagas disease vector, Triatoma infestans, at the urban-rural interface.

    Science.gov (United States)

    Foley, Erica A; Khatchikian, Camilo E; Hwang, Josephine; Ancca-Juárez, Jenny; Borrini-Mayori, Katty; Quıspe-Machaca, Victor R; Levy, Michael Z; Brisson, Dustin

    2013-10-01

    The increasing rate of biological invasions resulting from human transport or human-mediated changes to the environment has had devastating ecological and public health consequences. The kissing bug, Triatoma infestans, has dispersed through the Peruvian city of Arequipa. The biological invasion of this insect has resulted in a public health crisis, putting thousands of residents of this city at risk of infection by Trypanosoma cruzi and subsequent development of Chagas disease. Here, we show that populations of Tria. infestans in geographically distinct districts within and around this urban centre share a common recent evolutionary history although current gene flow is restricted even between proximal sites. The population structure among the Tria. infestans in different districts is not correlated with the geographical distance between districts. These data suggest that migration among the districts is mediated by factors beyond the short-range migratory capabilities of Tria. infestans and that human movement has played a significant role in the structuring of the Tria. infestans population in the region. Rapid urbanization across southern South America will continue to create suitable environments for Tria. infestans, and knowledge of its urban dispersal patterns may play a fundamental role in mitigating human disease risk.

  13. Population structure of the Chagas disease vector Triatoma infestans in an urban environment.

    Science.gov (United States)

    Khatchikian, Camilo E; Foley, Erica A; Barbu, Corentin M; Hwang, Josephine; Ancca-Juárez, Jenny; Borrini-Mayori, Katty; Quıspe-Machaca, Victor R; Naquira, Cesar; Brisson, Dustin; Levy, Michael Z

    2015-02-01

    Chagas disease is a vector-borne disease endemic in Latin America. Triatoma infestans, a common vector of this disease, has recently expanded its range into rapidly developing cities of Latin America. We aim to identify the environmental features that affect the colonization and dispersal of T. infestans in an urban environment. We amplified 13 commonly used microsatellites from 180 T. infestans samples collected from a sampled transect in the city of Arequipa, Peru, in 2007 and 2011. We assessed the clustering of subpopulations and the effect of distance, sampling year, and city block location on genetic distance among pairs of insects. Despite evidence of genetic similarity, the majority of city blocks are characterized by one dominant insect genotype, suggesting the existence of barriers to dispersal. Our analyses show that streets represent an important barrier to the colonization and dispersion of T. infestans in Arequipa. The genetic data describe a T. infestans infestation history characterized by persistent local dispersal and occasional long-distance migration events that partially parallels the history of urban development.

  14. Residual Infestation and Recolonization during Urban Triatoma infestans Bug Control Campaign, Peru1

    Science.gov (United States)

    Buttenheim, Alison M.; Pumahuanca, Maria-Luz Hancco; Calderón, Javier E. Quintanilla; Salazar, Renzo; Carrión, Malwina; Rospigliossi, Andy Catacora; Chavez, Fernando S. Malaga; Alvarez, Karina Oppe; Cornejo del Carpio, Juan; Náquira, César; Levy, Michael Z.

    2014-01-01

    Chagas disease vector control campaigns are being conducted in Latin America, but little is known about medium-term or long-term effectiveness of these efforts, especially in urban areas. After analyzing entomologic data for 56,491 households during the treatment phase of a Triatoma infestans bug control campaign in Arequipa, Peru, during 2003–2011, we estimated that 97.1% of residual infestations are attributable to untreated households. Multivariate models for the surveillance phase of the campaign obtained during 2009–2012 confirm that nonparticipation in the initial treatment phase is a major risk factor (odds ratio [OR] 21.5, 95% CI 3.35–138). Infestation during surveillance also increased over time (OR 1.55, 95% CI 1.15–2.09 per year). In addition, we observed a negative interaction between nonparticipation and time (OR 0.73, 95% CI 0.53–0.99), suggesting that recolonization by vectors progressively dilutes risk associated with nonparticipation. Although the treatment phase was effective, recolonization in untreated households threatens the long-term success of vector control. PMID:25423045

  15. An Updated Insight into the Sialotranscriptome of Triatoma infestans: Developmental Stage and Geographic Variations

    Science.gov (United States)

    Schwarz, Alexandra; Medrano-Mercado, Nora; Schaub, Günter A.; Struchiner, Claudio J.; Bargues, M. Dolores; Levy, Michael Z.; Ribeiro, José M. C.

    2014-01-01

    Background Triatoma infestans is the main vector of Chagas disease in South America. As in all hematophagous arthropods, its saliva contains a complex cocktail that assists blood feeding by preventing platelet aggregation and blood clotting and promoting vasodilation. These salivary components can be immunologically recognized by their vector's hosts and targeted with antibodies that might disrupt blood feeding. These antibodies can be used to detect vector exposure using immunoassays. Antibodies may also contribute to the fast evolution of the salivary cocktail. Methodology Salivary gland cDNA libraries from nymphal and adult T. infestans of breeding colonies originating from different locations (Argentina, Chile, Peru and Bolivia), and cDNA libraries originating from F1 populations of Bolivia, were sequenced using Illumina technology. Coding sequences (CDS) were extracted from the assembled reads, the numbers of reads mapped to these CDS, sequences were functionally annotated and polymorphisms determined. Main findings/Significance Over five thousand CDS, mostly full length or near full length, were publicly deposited on GenBank. Transcripts that were over 10-fold overexpressed from different geographical regions, or from different developmental stages were identified. Polymorphisms were mapped to derived coding sequences, and found to vary between developmental instars and geographic origin of the biological material. This expanded sialome database from T. infestans should be of assistance in future proteomic work attempting to identify salivary proteins that might be used as epidemiological markers of vector exposure, or proteins of pharmacological interest. PMID:25474469

  16. Genetic variability and microdistribution of Triatoma infestans genotypes and Trypanosoma cruzi clones in Arequipa region (Peru).

    Science.gov (United States)

    Brenière, S F; Lopez, J; Vargas, F; Barnabé, C

    1997-01-01

    The genetic variability of Triatoma infestans and Trypanosoma cruzi populations was studied by isoenzyme analysis in two distinct areas of Arequipa province (Peru); one, Santa Rita de Siguas, being an endemic area for Chagas' disease, the second, Arequipa, recently infected. Analysis of T. infestans genetic variability indicates, (i) temporal stability of genotypes found in Santa Rita de Siguas, (ii) high genetic differences between Arequipa and Santa Rita de Siguas populations suggesting minor contact between them, (iii) multiple origin of the T. infestans population in Arequipa, and (iv) poor dispersal capacity of T. infestans: the panmictic unit could be reduce to a house. Parasite isoenzyme analysis was performed in 29 Peruvian stocks of T. cruzi, mainly isolated from bugs taken in a single locality, Santa Rita de Siguas. The results show, (i) a high genetic polymorphism, (ii) nine different multilocus genotypes were detected and clustered in two different clades, (iii) most of the parasite isolates pertained to one of the clade and were genetically similar to those analyzed 12 years before. This sample allowed the study of the mating system of T. cruzi in strict sympathic conditions and gave more strength to the hypothesis of the clonal structure of T. cruzi populations.

  17. First record of Triatoma maculata (Erichson, 1848 (Hemiptera: Reduviidae: Triatomini in Riohacha, La Guajira – Colombia

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    Edith Natalia Gómez-Melendro

    2014-11-01

    Full Text Available Introduction. Knowledge of vector insect species, their habitat and geographical distribution is crucial for determining the risk of transmission of the etiologic agents that cause disease in humans, which allows defining strategies for prevention, surveillance and control in line with the characteristics of each area. Objective. To determine the presence and public health importance of vectors of Chagas disease in the indigenous settlements of Marbacella and El Horno of the Wayúu ethnic group in the municipality of Riohacha, La Guajira, Colombia. Materials and methods. From active search, installation and inspection of biosensors and occasional catches, Hemiptera: Reduviidae: Triatomini were collected intra and in the peridomicile housing of the indigenous settlements of El Horno and Marbacella of the the Wayúu ethnic group. Indices of intra and peridomestic infestation, colonization, density, dispersion and natural infection with Trypanosoma cruzi Chagas, 1909 were calculated. Results. 79.6% (n = 90 of the specimens were collected around the homes and 20.3% (n = 23 inside the homes, all corresponding to Triatoma maculata (Erichson, 1848. The natural infection indices with T. cruzi accounted for 43.5% for Marbacella and 36% for El Horno. Conclusion. This is the first reported capture of individuals of T. maculata, considered a secondary vector of Chagas disease in Colombia, naturally infected with T. cruzi in the municipality of Riohacha expanding the geographical distribution of the species in the department of La Guajira.

  18. An updated insight into the Sialotranscriptome of Triatoma infestans: developmental stage and geographic variations.

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    Alexandra Schwarz

    2014-12-01

    Full Text Available Triatoma infestans is the main vector of Chagas disease in South America. As in all hematophagous arthropods, its saliva contains a complex cocktail that assists blood feeding by preventing platelet aggregation and blood clotting and promoting vasodilation. These salivary components can be immunologically recognized by their vector's hosts and targeted with antibodies that might disrupt blood feeding. These antibodies can be used to detect vector exposure using immunoassays. Antibodies may also contribute to the fast evolution of the salivary cocktail.Salivary gland cDNA libraries from nymphal and adult T. infestans of breeding colonies originating from different locations (Argentina, Chile, Peru and Bolivia, and cDNA libraries originating from F1 populations of Bolivia, were sequenced using Illumina technology. Coding sequences (CDS were extracted from the assembled reads, the numbers of reads mapped to these CDS, sequences were functionally annotated and polymorphisms determined.Over five thousand CDS, mostly full length or near full length, were publicly deposited on GenBank. Transcripts that were over 10-fold overexpressed from different geographical regions, or from different developmental stages were identified. Polymorphisms were mapped to derived coding sequences, and found to vary between developmental instars and geographic origin of the biological material. This expanded sialome database from T. infestans should be of assistance in future proteomic work attempting to identify salivary proteins that might be used as epidemiological markers of vector exposure, or proteins of pharmacological interest.

  19. Molecular epidemiology of Trypanosoma cruzi and Triatoma dimidiata in costal Ecuador.

    Science.gov (United States)

    Wong, Yim Yan; Sornosa Macias, Karen Jeniffer; Guale Martínez, Doris; Solorzano, Luis F; Ramirez-Sierra, Maria Jesus; Herrera, Claudia; Dumonteil, Eric

    2016-07-01

    Chagas disease is a neglected tropical disease caused by the protozoan parasite Trypanosoma cruzi. In Ecuador, Triatoma dimidiata and Rhodnius ecuadoriensis are the main vector species, responsible for over half of the cases of T. cruzi infection in the country. T. dimidiata is believed to have been introduced in Ecuador during colonial times, and its elimination from the country is thus believed to be feasible. We investigated here the molecular ecology of T. dimidiata and T. cruzi in costal Ecuador to further guide control efforts. Analysis of the Internal Transcribed Spacer 2 (ITS-2) of 23 specimens from Progreso, Guayas, unambiguously supported the likely importation of T. dimidiata from Central America to Ecuador. The observation of a very high parasite infection rate (54%) and frequent feeding on humans (3/5) confirmed a continued risk of transmission to humans. All genotyped parasites corresponded to TcI DTU and Trypanosoma rangeli was not detected in T. dimidiata. TcI subgroups corresponded to TcIa (25%), and mixed infections with TcIa and TcId (75%). Further studies should help clarify T. cruzi genetic structure in the country, and the possible impact of the introduction of T. dimidiata on the circulating parasite strains. The elevated risk posed by this species warrants continuing efforts for its control, but its apparent mobility between peridomestic and domestic habitats may favor reinfestation following insecticide spraying.

  20. Insecticide and community interventions to control Triatoma dimidiata in localities of the State of Veracruz, Mexico

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    Gloria Rojas Wastavino

    2004-06-01

    Full Text Available Three different interventions to control Triatoma dimidiata in the State of Veracruz were implemented: X-1 = whole dwelling spraying, X-2 = middle wall spraying, X-3 = household cleaning. Cyfluthrin was sprayed 3 times with 8 month intervals. After each spraying, insects were collected and sent to the laboratory to be recorded and to determine genus and species of the adult triatomine bugs, and nymphs were counted. Trypanosoma cruzi presence was determined. With X-1, the infestation, colonization, and natural infection indexes were reduced to 0% in the 3 localities, with respect to t0. With X-2, the infestation index was reduced to 10% at t3 in 3 localities; the colonization index was reduced to 0% in only 1 locality at t3, and the natural infection index was reduced to 0% at t3. With X-3 the 3 indexes were not effectively reduced but they decreased with respect to the baseline study. Insecticide application to the whole dwelling is a more efficient intervention than its application to only the lower half of the walls and to the cleaning of houses.

  1. Differential expression profiles in the midgut of Triatoma infestans infected with Trypanosoma cruzi.

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    Diego S Buarque

    Full Text Available Chagas disease, or American trypanosomiasis, is a parasitic disease caused by the protozoan Trypanosoma cruzi and is transmitted by insects from the Triatominae subfamily. To identify components involved in the protozoan-vector relationship, we constructed and analyzed cDNA libraries from RNA isolated from the midguts of uninfected and T. cruzi-infected Triatoma infestans, which are major vectors of Chagas disease. We generated approximately 440 high-quality Expressed Sequence Tags (ESTs from each T. infestans midgut cDNA library. The sequences were grouped in 380 clusters, representing an average length of 664.78 base pairs (bp. Many clusters were not classified functionally, representing unknown transcripts. Several transcripts involved in different processes (e.g., detoxification showed differential expression in response to T. cruzi infection. Lysozyme, cathepsin D, a nitrophorin-like protein and a putative 14 kDa protein were significantly upregulated upon infection, whereas thioredoxin reductase was downregulated. In addition, we identified several transcripts related to metabolic processes or immunity with unchanged expressions, including infestin, lipocalins and defensins. We also detected ESTs encoding juvenile hormone binding protein (JHBP, which seems to be involved in insect development and could be a target in control strategies for the vector. This work demonstrates differential gene expression upon T. cruzi infection in the midgut of T. infestans. These data expand the current knowledge regarding vector-parasite interactions for Chagas disease.

  2. HETEROCHROMATIC CHROMOSOMES IN TRIATOMA INFESTANS (KLUG, 1834 IN THE SOUTH OF PERU

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    Guevara, Yenny

    2011-01-01

    Full Text Available Heterochromatic regions in triatomines chromosomes that include heterochromatin's quantity, distribution, nature and behavior have allowed finding a wide intra and interpopulation variation. Triatoma infestans (Klug, 1834, has relatively huge blocks of heterochromatin in some of its chromosomal pairs; three clearly different chromosomal groups have been identified for this specie nowadays at South America. Thus, we have the nominated “andean” group (composed of individuals from Peruvian and Bolivian Andean regions which has between 14 and 20 heterochromatic chromosomes; the “not andean” group (populations from Argentina, Paraguay, Brazil, Uruguay and Bolivian Chaco which has from 4 to 7 heterochromatic chromosomes; the “Intermiddle” group at the north extreme of Argentina (Salvador Mazza, Salta province and southern Bolivia (Tarija department which has from 7 to 11 heterochromatic chromosomes. The aim of this study was to identify the presence of heterochromatic chromosomes at two population samples of T. infestans from southern Peru regions to identify the genomic group where they belong to. Squash technique and banding C staining of Sumner, modified by Panzera were used with male individuals from Santa Rita de Siguas (Arequipa and Nazca (Ica locations. “Andean” heterochromatic chromosomes with “not andean” group particularities have been found at individuals from both locations.

  3. Isoenzymes Detect Variation in Populations of Triatoma brasiliensis (Hemiptera: Reduviidae: Triatominae

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    Costa Jane

    1997-01-01

    Full Text Available Triatoma brasiliensis is one of the most important vectors of Chagas disease in the semiarid zone of the northeast of Brazil. Intraspecific morphological and behavioural variation has been reported for different populations. Results for four distinct populations using eight isoenzymes are reported here. The literature describes three subspecies: T. brasiliensis brasiliensis Neiva, 1911; T. brasiliensis melanica Neiva & Lent, 1941 and T. brasiliensis macromelasoma Galvão, 1956. These subspecies differ mainly in their cuticle colour pattern and were regarded as synonyms by Lent and Wygodzinsky (1979. In order to evaluate whether the chromatic pattern is a morphological variation of different melanic forms within T. brasiliensis or due to interspecific variation, field collections were performed in localities where these three subspecies have been described: Caicó (Rio Grande do Norte, the type-locality for T. b. brasiliensis; Petrolina (Pernambuco for T. b. macromelasoma and Espinosa (Minas Gerais for T. b. melanica. A fourth distinct chromatic pattern was found in Juazeiro (Bahia. A total of nine loci were studied. Values of Nei's genetic distance (D were calculated. T. b. brasiliensis and T. b. macromelasoma are the closest populations with a D=0.295. T. b. melanica had a D ³ 0.537 when compared to the others, a distance in the range of interspecific variation for other triatomine species

  4. Geographic distribution and morphometric differentiation of Triatoma nitida usinger 1939 (Hemiptera: Reduviidae: Triatominae in Guatemala

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    Monroy Carlota

    2003-01-01

    Full Text Available Triatoma nitida was found in 14 (0.4% out of 3,726 houses located in six departments across Guatemala, which were surveyed from 1994 to 1998 by the man-hour collection method. Compared to previous information, the distribution of T. nitida in Guatemala has increased from five to nine departments; the species is present in mild climates at altitudes from 960 to 1,500 m. Fourteen percent of the intradomestic T. nitida were infected with Trypanosoma cruzi. The species was often found in conjunction with other triatomines (T. dimidiata and Rhodnius prolixus. The domestic and peridomestic presence of T. nitida in Guatemala was rare, but occasionally this species was colonizing human-made constructions. T. nitida appears to have a low importance as Chagas disease vector in Guatemala, as indicated by its scarce presence in the domestic habitats and defecation patterns. However, it clearly has potential to become a Chagas vector so we recommend an on-going study of the intradomestic presence of T. nitida following the control programs in Guatemala. Morphometric analysis of 47 T. nitida males from three localities showed quantitative differences between the populations, which indicates that geographic distance is an important factor in the structuring of T. nitida populations.

  5. The interaction between Poultry and Triatoma infestans Klug, 1834 (Hemiptera: Reduviidae in an experimental model

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    Nicolás J. Schweigmann

    1995-06-01

    Full Text Available This paper compares the predation pressure that ducks and chickens exert on triatomines. For the tests, these birds were placed in individual boxes together with a known number of Triatoma infestans and left to interact from 6 p.m. till the next morning, involving a long lasting period of complete darkness limited by two short-term periods of semi-darkness. There was a shelter which could prevent the bugs from being predated. The number of live and dead triatomines was recorded, considering missing bugs as predated by the birds. Ducks exhibited a greater predatory activity than chickens, that could be due to a long term active period at night while chickens sleep motionless from sunset to dawn. Surviving triatomines that had fed on chickens outnumbered those fed on ducks suggesting that these were less accessible to the triatomine biting. If ducks are better than chickens to detect and eat bugs and to interfere with their feeding in the field, an increase in duck number might help to diminish triatomine density. Further research is needed to determine the feasibility of application of these experimental results.

  6. Genetic structure of Triatoma venosa (Hemiptera: Reduviidae: molecular and morphometric evidence

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    Erika Vargas

    2006-02-01

    Full Text Available Triatoma venosa presents a restricted geographical distribution in America and is considered as a secondary vector of Chagas disease in Colombia and Ecuador. A total of 120 adult insects were collected in domestic and peridomestic habitats in an endemic area of the department of Boyacá, Colombia, in order to determine their genetic structure through morphometric and molecular techniques. The head and wings of each specimen were used for the analyses of size, shape, and sexual dimorphism. A significant sexual dimorphism was found, although no differences in size among the studied groups were detected. Differences were found in the analyzed structures except for male heads. DNA was extracted from the legs in order to carry out the internal transcriber space-2 (ITS-2 amplification and the randon amplified polymorphic DNA (RAPD analyses. Length polymorphisms were not detected in the ITS-2. Fst and Nm values were estimated (0.047 and 3.4, respectively. The high genetic flow found among the insects captured in the domicile and peridomiciliary environment does not permit a genetic differentiation, thus establishing the peridomicile as an important place for epidemiological surveillance.

  7. Triatoma mexicana (Hemiptera: Reduviidae in Guanajuato, Mexico: house infestation and seasonal variation

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    Paz María Salazar Schettino

    2007-11-01

    Full Text Available Triatoma mexicana was described by Herrich-Schaeffer in 1848. In 1940, a male specimen was found in Hidalgo. In 1970, this species was recorded in the state of Queretaro. Later, it was registered in Guanajuato and San Luis Potosi. In the present paper we performed an investigation in 545 dwellings from three counties in the state of Guanajuato, Mexico, from March 2003 to May 2004. The search and capture of triatomines were seasonally performed indoors and outdoors. Entomological indexes were calculated. The risk and no risk relations between triatomine presence and housing construction materials were analyzed. Fourteen triatomines were collected indoors and 151 outdoors. The vectors were collected in houses built with either risky and non-risky materials. Adults go indoors but do not settle there, hence, no relationship was found between the building materials and infestation of houses. Conventional interventions like house improvement or insecticide spraying are not efficient for the control of T. mexicana, because its developmental cycle is accomplished outdoors in the area surrounding the houses.

  8. Selection of Beauveria bassiana and Metarhizium anisopliae Isolates to Control Triatoma infestans

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    Luz Christian

    1998-01-01

    Full Text Available Twenty three isolates of Beauveria bassiana and 13 isolates of Metarhizium anisopliae were tested on third instar nymphs of Triatoma infestans, a serious vector of Chagas disease. Pathogenicity tests at saturated humidity showed that this insect is very susceptible to fungal infection. At lower relative humidity (50%, conditions expected in the vector microhabitat, virulence was significantly different among isolates. Cumulative mortality 15 days after treatment varied from 17.5 to 97.5%, and estimates of 50% survival time varied from 6 to 11 days. Maintaining lower relative humidity, four B. bassiana and two M. anisopliae isolates were selected for analysis of virulence at different conidial concentrations and temperatures. Lethal concentrations sufficient to kill 50% of insects (LC50 varied from 7.1x105 to 4.3x106 conidia/ml, for a B. bassiana isolate (CG 14 and a M. anisopliae isolate (CG 491 respectively. Most isolates, particularly B. bassiana isolates CG 24 and CG 306, proved to be more virulent at 25 and 30°C, compared to 15 and 20°C. The differential virulence at 50% humidity observed among some B. bassiana isolates was not correlated to phenetic groups in cluster analysis of RAPD markers. In fact, the B. bassiana isolates analyzed presented a high homogeneity (> 73% similarity.

  9. Invasion speeds of Triatoma dimidiata, vector of Chagas disease: An application of orthogonal polynomials method.

    Science.gov (United States)

    Mesk, Mohammed; Mahdjoub, Tewfik; Gourbière, Sébastien; Rabinovich, Jorge E; Menu, Frédéric

    2016-04-21

    Demographic processes and spatial dispersal of Triatoma dimidiata, a triatomine species vector of Chagas disease, are modeled by integrodifference equations to estimate invasion capacity of this species under different ecological conditions. The application of the theory of orthogonal polynomials and the steepest descent method applied to these equations, allow a good approximation of the abundance of the adult female population and the invasion speed. We show that: (1) under the same mean conditions of demography and dispersal, periodic spatial dispersal results in an invasion speed 2.5 times larger than the invasion speed when spatial dispersal is continuous; (2) when the invasion speed of periodic spatial dispersal is correlated to adverse demographic conditions, it is 34.7% higher as compared to a periodic dispersal that is correlated to good demographic conditions. From our results we conclude, in terms of triatomine population control, that the invasive success of T. dimidiata may be most sensitive to the probability of transition from juvenile to adult stage. We discuss our main theoretical predictions in the light of observed data in different triatomines species found in the literature.

  10. Population Structure of the Chagas Disease Vector Triatoma infestans in an Urban Environment

    Science.gov (United States)

    Khatchikian, Camilo E.; Foley, Erica A.; Barbu, Corentin M.; Hwang, Josephine; Ancca-Juárez, Jenny; Borrini-Mayori, Katty; Quıspe-Machaca, Victor R.; Naquira, Cesar; Brisson, Dustin; Levy, Michael Z.

    2015-01-01

    Chagas disease is a vector-borne disease endemic in Latin America. Triatoma infestans, a common vector of this disease, has recently expanded its range into rapidly developing cities of Latin America. We aim to identify the environmental features that affect the colonization and dispersal of T. infestans in an urban environment. We amplified 13 commonly used microsatellites from 180 T. infestans samples collected from a sampled transect in the city of Arequipa, Peru, in 2007 and 2011. We assessed the clustering of subpopulations and the effect of distance, sampling year, and city block location on genetic distance among pairs of insects. Despite evidence of genetic similarity, the majority of city blocks are characterized by one dominant insect genotype, suggesting the existence of barriers to dispersal. Our analyses show that streets represent an important barrier to the colonization and dispersion of T. infestans in Arequipa. The genetic data describe a T. infestans infestation history characterized by persistent local dispersal and occasional long-distance migration events that partially parallels the history of urban development. PMID:25646757

  11. Morphological and Genetic Differentiation within the Southernmost Vector of Chagas Disease: Triatoma patagonica (Hemiptera – Reduviidae)

    Science.gov (United States)

    Pita, Sebastián; Calleros, Lucía; Crocco, Liliana; Panzera, Yanina; Rodríguez, Claudia S.; Panzera, Francisco

    2016-01-01

    The epidemiological importance of Chagas disease vectors largely depends on their spreading ability and adaptation to domestic habitats. Triatoma patagonica is a secondary vector of Chagas disease endemic of Argentina, and it has been found colonizing domiciles and most commonly peridomiciliary structures in several Argentine provinces and morphological variation along its distribution range have been described. To asses if population differentiation represents geographic variants or true biological species, multiple genetic and phenotypic approaches and laboratory cross-breeding were performed in T. patagonica peridomestic populations. Analyses of chromatic variation of forewings, their size and the content of C-heterochromatin on chromosomes revealed that populations are structured following a North-South latitudinal variation. Cytochrome c oxidase I mitochondrial gene (COI) nucleotide analysis showed a mean genetic distance of 5.2% between the most distant populations. The cross-breeding experiments suggest a partial reproductive isolation between some populations with 40% of couples not laying eggs and low hatching efficiency. Our findings reveal phenotypic and genetic variations that suggest an incipient differentiation processes among T. patagonica populations with a pronounced phenotypic and genetic divergence between the most distant populations. The population differentiation here reported is probably related to differential environmental conditions and it could reflect the occurrence of an incipient speciation process in T. patagonica. PMID:28005972

  12. Morphological and Genetic Differentiation within the Southernmost Vector of Chagas Disease: Triatoma patagonica (Hemiptera - Reduviidae).

    Science.gov (United States)

    Nattero, Julieta; Pita, Sebastián; Calleros, Lucía; Crocco, Liliana; Panzera, Yanina; Rodríguez, Claudia S; Panzera, Francisco

    2016-01-01

    The epidemiological importance of Chagas disease vectors largely depends on their spreading ability and adaptation to domestic habitats. Triatoma patagonica is a secondary vector of Chagas disease endemic of Argentina, and it has been found colonizing domiciles and most commonly peridomiciliary structures in several Argentine provinces and morphological variation along its distribution range have been described. To asses if population differentiation represents geographic variants or true biological species, multiple genetic and phenotypic approaches and laboratory cross-breeding were performed in T. patagonica peridomestic populations. Analyses of chromatic variation of forewings, their size and the content of C-heterochromatin on chromosomes revealed that populations are structured following a North-South latitudinal variation. Cytochrome c oxidase I mitochondrial gene (COI) nucleotide analysis showed a mean genetic distance of 5.2% between the most distant populations. The cross-breeding experiments suggest a partial reproductive isolation between some populations with 40% of couples not laying eggs and low hatching efficiency. Our findings reveal phenotypic and genetic variations that suggest an incipient differentiation processes among T. patagonica populations with a pronounced phenotypic and genetic divergence between the most distant populations. The population differentiation here reported is probably related to differential environmental conditions and it could reflect the occurrence of an incipient speciation process in T. patagonica.

  13. House-level risk factors for Triatoma dimidiata infestation in Colombia.

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    Parra-Henao, Gabriel; Cardona, Ángela Segura; Quirós-Gómez, Oscar; Angulo, Víctor; Alexander, Neal

    2015-01-01

    In Colombia, the main vectors of Trypanosoma cruzi, the causative agent of Chagas disease, are Rhodnius prolixus and Triatoma dimidiata. T. dimidiata is present in the east region of Colombia as domestic, peridomestic, and sylvatic populations, resulting in difficulties for its control. A cost-effective way to prioritize houses for treatment is to stratify houses based on risk factors. In this study, risk factors were evaluated for potential associations with domicile infestation of T. dimidiata. There was an increased likelihood of domestic infestation associated with the presence of mixed roofs (odds ratio [OR] = 36.14, 95% confidence interval [95% CI] = 12.21-106.97), cats (OR = 3.94, 95% CI = 1.36-11.38), rock piles (OR = 5.28, 95% CI = 1.64-16.98), and bushes with height above 10 m (OR = 11.21, 95% CI = 2.08-60.45). These factors could be used to target surveillance and control of T. dimidiata to houses with an increased risk of being infested.

  14. Daily rhythm of aggregation in the haematophagous bug Triatoma infestans (Heteroptera: Reduviidae

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    S A Minoli

    2007-06-01

    Full Text Available Triatomine bugs show a temporal modulation of many activities. Here, we analyse the daily modulation of the aggregation behaviour of Triatoma infestans larvae and its chronobiological basis. In the laboratory, groups of six bugs were released over an experimental arena during six consecutive days, where their aggregation behaviour was quantified every hour. When submitted to a 12/12 h photoperiod (L/D, the larvae of T. infestans exhibited a cyclic pattern of aggregation with a 24 h period, evincing the existence of a daily rhythm of aggregation in this species. Bugs exhibited the maximum aggregation tendency at the end of the scotophase (7:00 h, moment in which they naturally search for refuges. The minimum aggregation (i.e. maximal dispersion was observed during the last part of the photophase and beginning of the scotophase (15:00 to 1:00 h. This cyclic pattern disappeared when constant conditions of illumination (L/L or darkness (D/D were imposed to the bugs, suggesting the absence of an endogenous circadian control of this behaviour. Insects submitted to L/L and D/D photoperiods presented lower global levels of aggregation than those submitted to L/D conditions. The lack of an endogenous control and the relevance of light cycles as a synchronization signal are discussed as the temporal modulation of this behaviour might play an important role in the nocturnal habits of this species.

  15. Diatomaceous earth and oil enhance effectiveness of Metarhizium anisopliae against Triatoma infestans.

    Science.gov (United States)

    Luz, Christian; Rodrigues, Juscelino; Rocha, Luiz F N

    2012-04-01

    Entomopathogenic fungi, especially Metarhizium anisopliae, have potential for integrated control of peridomestic triatomine bugs. However, the high susceptibility of these vectors to fungal infection at elevated ambient humidities decreases in the comparatively dry conditions that often prevail in their microhabitats. A formulation adapted to this target pest that induces high and quick mortality can help to overcome these drawbacks. In the present study diatomaceous earth, which is used against pests of stored grains or as an additive to mycoinsecticides, delayed but did not reduce in vitro germination of M. anisopliae s.l. IP 46 conidia after >24h agitation without affecting viability, and did not hamper the survival of Triatoma infestans nymphs exposed to treated surfaces. The settling behavior of nymphs on a treated surface in choice tests depended on the concentration of diatomaceous earth and ambient light level. Conidia formulated with diatomaceous earth and a vegetable oil synergized the insecticidal effect of the fungus in nymphs, and quickly killed all treated insects, even at 75% relative humidity (LT(90) 8.3 days) where unformulated conidia caused only 25% mortality after a 25 days exposure. The improved performance of a combined oil and desiccant dust formulation of this Metarhizium isolate raises the likelihood for its successful mycoinsecticidal use for triatomine control and, apparently, against other domestic insect pests.

  16. Bias due to methods of parasite detection when estimating prevalence of infection of Triatoma infestans by Trypanosoma cruzi

    OpenAIRE

    Lardeux, Frédéric; Aliaga, C.; Depickère, Stéphanie

    2016-01-01

    The study aimed to quantify the bias from parasite detection methods in the estimation of the prevalence of infection of Triatoma infestans by Trypanosoma cruzi, the agent of Chagas disease. Three common protocols that detect T. cruzi in a sample of 640 wild-caught T. infestans were compared: (1) the microscopic observation of insect fecal droplets, (2) a PCR protocol targeting mini-exon genes of T. cruzi (MeM-PCR), and (3) a PCR protocol targeting a satellite repeated unit of the parasite. A...

  17. [Triatoma delpontei Romaña & Abalos, 1947 (Hemiptera, Tratominae) in the Brazilian State of Rio Grande do Sul].

    Science.gov (United States)

    Salvatella Agrelo, R; Basmadjian, Y; Rosa, R; Puime, A

    1993-01-01

    Triatoma delpontei (Romaña & Abalos, 1947) (Hemiptera, Triatominae) is an ornithophilic sylvatic with a particular association to the psittacid Myiopsitta monachus (Boaddert, 1783). It is found in the continental biogeographical province of the Chaco, where it inhabits the nests or M. monachus, in subtropical xerophytic forests. The authors report the first finding of T. delpontei in Brasil, in the "campanha" region of the State or Rio Grande do Sul (Barra do Quarai, Uruguaiana), on the right bank of the River Cuareim, not far from de Uruguayan border.

  18. Hallazgo de Triatoma platensis Neiva, 1913 (Hemiptera, Triatominae) en el estado brasileño de "Rio Grande do Sul"

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    Salvatella,Roberto; Basmadjián,Yester; Rosa,Raquel; Martínez, María; Mendaro,Gabriela; Civila,Eduardo

    1991-01-01

    Triatoma platensis Neiva 1913 (Hemiptera, Triatominae), especie ornitófila, con área de dispersión conocida en Argentina, Bolivia, Paraguay y Uruguay es notificada en un primer registro para Brasil. Hallada en el municipio de Uruguaiana (Río Grande do Sul) en nidos de Anumbius annumbi (Vieillot, 1817) (Passeriformes, Furnariidae) donde cohabitaba con cricétidos de la especie Orizomys flavescens. Ningún ejemplar de los siete colectados se presentó infectado por Trypanosoma cruzi.

  19. First record of Megaselia scalaris (Loew) (Diptera: Phoridae) infesting laboratory colonies of Triatoma brasiliensis Neiva (Hemiptera: Reduviidae)

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Jane; Almeida, Carlos E.; Esperanca, Gleidson M.; Morales, Ninive [Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ (Brazil). Dept.de Entomologia. Lab. da Colecao Entomologica; Mallet, Jacenir R. dos S.; Goncalves, Teresa C.M. [Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ (Brazil). Lab. Transmissores de Leishmanioses. Nucleo de Ultraestrutura; Prado, Angelo P. do [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Inst. de Biologia. Dept. de Parasitologia

    2007-11-15

    Megaselia scalaris (Loew) is a cosmopolitan and synanthropic scuttle fly, eclectic in its feeding habits and acts as detritivore, parasite, facultative parasite, and parasitoid. Here we report for the first time M. scalaris infesting laboratory colonies of Triatoma brasiliensis Neiva, the most important Chagas disease vector in semiarid areas of Brazil. M. scalaris larvae were found feeding inside bugs; pupae were found in the esophagus and intestinal regions of T. brasiliensis through dissection. Other relevant information about this finding is also described in this note, including some preventive measures to avoid laboratory colonies infestations. (author)

  20. Triatoma delpontei Romaña & Abalos, 1947 (Hemiptera, Triatominae en el estado brasileño de "Rio Grande do Sul" Triatoma delpontei Romaña & Abalos, 1947 (Hemiptera, Triatominae in Rio Grande do Sul State, Brasil

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    Roberto Salvatella Agrelo

    1993-02-01

    Full Text Available Triatoma delpontei Romaña y Abalos, 1947 es una especie de triatomineo silvestre, de marcada ornitofilia, que se asocia preferentemente al psitácido Myiopsitta monachus (Boddaert, 1783 colonizando sus nidos. Especie que se identifica con zonas de forestas subtropicales secas o xerofíticas propias de las provincias biogeográficas continentales chaqueñas, es notificada para Brasil en la microregión de la campaña de Río Grande do Sul (barra de Quaraí, Municipio de Uruguaiana.Triatoma delpontei (Romaña & Abalos, 1947 (Hemiptera, Triatominae is an ornithophilic sylvatic with a particular association to the psittacid Myiopsitta monachus (Boaddert, 1783. It is found in the continental biogeographical province of the Chaco, where it inhabits the nests or M.monachus, in subtropical xerophytic forests. The authors report the first finding of T. delpontei in Brasil, in the "campanha" region of the State or Rio Grande do Sul (Barra do Quarai, Uru-guaiana, on the right bank of the River Cuareim, not far from de Uruguayan border.

  1. Contribuição ao estudo da distribuição geográfica de triatomíneos no estado do Piauí

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    Paulo Zábulon de Figueirêdo

    1975-08-01

    Full Text Available Os autores apresentam a atual distribuição geográfica dos triatomineos no Estado do Piauí. Dos 28 municípios trabalhados em três diferentes regiões naturais, 18 apresentaram capturas positivas, com as seguintes espécies: Triatoma brasiliensis, T. maculata, T. sórdida, Rhodnius pictepes, R. neglectus e Panstrongylus geniculatus. Das espécies citadas, o T. brasiliensis foi a mais freqüente e de maior distribuição. Os autores admitem ser essa espécie a mais importante transmissora da Doença de Chagas, no Estado. Chamam a atenção, ainda, para o papel que as espécies de R. pictepes e R. neglectus possam desempenhar na manutenção do ciclo silvestre do Trypanosoma cruzi, ao lado do P. geniculatus.

  2. Geographic variation of Trypanosoma cruzi discrete typing units from Triatoma infestans at different spatial scales.

    Science.gov (United States)

    Fernández, María Del Pilar; Cecere, María Carla; Lanati, Leonardo Alejandro; Lauricella, Marta Alicia; Schijman, Alejandro Gabriel; Gürtler, Ricardo Esteban; Cardinal, Marta Victoria

    2014-12-01

    We assessed the diversity and distribution of Trypanosoma cruzi discrete typing units (DTU) in Triatoma infestans populations and its association with local vector-borne transmission levels at various geographic scales. At a local scale, we found high predominance (92.4%) of TcVI over TcV in 68 microscope-positive T. infestans collected in rural communities in Santiago del Estero province in northern Argentina. TcV was more often found in communities with higher house infestation prevalence compatible with active vector-borne transmission. Humans and dogs were the main bloodmeal sources of the TcV- and TcVI-infected bugs. At a broader scale, the greatest variation in DTU diversity was found within the Argentine Chaco (227 microscope-positive bugs), mainly related to differences in equitability between TcVI and TcV among study areas. At a country-wide level, a meta-analysis of published data revealed clear geographic variations in the distribution of DTUs across countries. A correspondence analysis showed that DTU distributions in domestic T. infestans were more similar within Argentina (dominated by TcVI) and within Bolivia (where TcI and TcV had similar relative frequencies), whereas large heterogeneity was found within Chile. DTU diversity was lower in the western Argentine Chaco region and Paraguay (D=0.14-0.22) than in the eastern Argentine Chaco, Bolivia and Chile (D=0.20-0.68). Simultaneous DTU identifications of T. cruzi-infected hosts and triatomines across areas differing in epidemiological status are needed to shed new light on the structure and dynamics of parasite transmission cycles.

  3. Experience-dependent modulation of the attraction to faeces in the kissing bug Triatoma infestans.

    Science.gov (United States)

    Mengoni, Sofía L; Lorenzo-Figueiras, Alicia N; Minoli, Sebastián A

    2016-11-11

    Triatoma infestans is the main vector of the Chagas disease in Latin America. These nocturnal bugs spend most of the daylight hours aggregated with conspecifics inside crevices in roofs and walls. Around the entrances of the shelters T. infestans deposits faeces that contain chemical cues that attract conspecifics. In this work we investigated whether attraction to faeces can be modulated by experience in this insect species. First, we analyzed if the attraction of nymphs to faeces is innate or acquired through previous sensory experiences. Results show that after hatching, 1st instar nymphs are attracted to faeces even if they had never been in contact with them before, thus indicating that this attraction is innate. Second, we studied if attraction to faeces can be influenced by the presence of con-specifics. No differences were found in the attraction to faeces of nymphs released alone or in groups, suggesting that attraction to faeces is independent of the presence of other individuals. Third, we examined if the innate response to faeces of nymphs can be modulated by experience. After pre-exposing nymphs to faeces during 24h, insects were no longer attracted to faeces. Finally, by pairing the presence of faeces with an aversive mechanical disturbance, nymphs switched from attraction to avoidance of faeces. These results show that although faeces attraction has a strong innate component, it can be modulated by experience. The learning and memory capacities of triatomines have been studied only recently, and our work is the first report on the effects of experience in the aggregation context.

  4. Antennal phenotype of Mexican haplogroups of the Triatoma dimidiata complex, vectors of Chagas disease.

    Science.gov (United States)

    May-Concha, Irving; Guerenstein, Pablo G; Ramsey, Janine M; Rojas, Julio C; Catalá, Silvia

    2016-06-01

    Triatoma dimidiata (Latreille) is a species complex that spans North, Central, and South America and which is a key vector of all known discrete typing units (DTU) of Trypanosoma cruzi, the etiologic agent of Chagas disease. Morphological and genetic studies indicate that T. dimidiata is a species complex with three principal haplogroups (hg) in Mexico. Different markers and traits are still inconclusive regarding if other morphological differentiation may indicate probable behavioral and vectorial divergences within this complex. In this paper we compared the antennae of three Mexican haplogroups (previously verified by molecular markers ND4 and ITS-2) and discussed possible relationships with their capacity to disperse and colonized new habitats. The abundance of each type of sensillum (bristles, basiconics, thick- and thin-walled trichoids) on the antennae of the three haplogroups, were measured under light microscopy and compared using Kruskal-Wallis non-parametric and multivariate non-parametric analyses. Discriminant analyses indicate significant differences among the antennal phenotype of haplogroups either for adults and some nymphal stages, indicating consistency of the character to analyze intraspecific variability within the complex. The present study shows that the adult antennal pedicel of the T. dimidiata complex have abundant chemosensory sensilla, according with good capacity for dispersal and invasion of different habitats also related to their high capacity to adapt to conserved as well as modified habitats. However, the numerical differences among the haplogroups are suggesting variations in that capacity. The results here presented support the evidence of T. dimidiata as a species complex but show females and males in a different way. Given the close link between the bug's sensory system and its habitat and host-seeking behavior, AP characterization could be useful to complement genetic, neurological and ethological studies of the closely

  5. Phenotypic plasticity in response to food source in Triatoma infestans (Klug, 1834) (Hemiptera, Reduviidae: Triatominae).

    Science.gov (United States)

    Nattero, Julieta; Malerba, Romina; Rodríguez, Claudia S; Crocco, Liliana

    2013-10-01

    In the Gran Chaco region of Argentina, Bolivia, and Paraguay, vector transmission of Trypanosoma cruzi, the etiological agent of Chagas disease, is still a severe problem because, among other causes, houses are reinfested with Triatoma infestans, the main vector of T. cruzi in southern South America. A better understanding of adaptation and evolution of T. infestans populations may contribute to the selection of appropriate vector control strategies in this region. Phenotypic plasticity is essential to understand development and maintenance of morphological variation. An experimental phenotypic plasticity study was conducted to assess if blood meal source induced head shape and size variation during development in T. infestans. Eighteen full-sib families were assigned to one of two food sources (pigeon and guinea pig) to examine the effect of food source on head shape and size in all nymph instars and adults. Data were analyzed using geometric morphometric tools and phenotypic plasticity analyses. Significant differences in head shape and size were observed between adults fed on different food sources. Allometric effects at the adult stage were observed. Head size showed significant food source × family interaction for fifth-instar nymphs and adults. For head size, significant differences between food sources were observed at stages and in ontogenetic trajectory. Phenotypic plasticity expression was found for head shape and size in adults; indeed, bugs fed on guinea pigs exhibited greater changes in head shape and larger heads than those fed on pigeon. Full-sib families exhibited different patterns of phenotypic expression in response to food source. Food source × family interaction may indicate that the observed variation in phenotypic plasticity may contribute to changes in head morphometry. These results may contribute to the selection of an appropriate control strategy for T. infestans in the Gran Chaco region, since they provide evidences of morphological

  6. Ecological, social and biological risk factors for continued Trypanosoma cruzi transmission by Triatoma dimidiata in Guatemala.

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    Dulce M Bustamante

    Full Text Available BACKGROUND: Chagas disease transmission by Triatoma dimidiata persists in Guatemala and elsewhere in Central America under undefined ecological, biological and social (eco-bio-social conditions. METHODOLOGY: Eco-bio-social risk factors associated with persistent domiciliary infestation were identified by a cross-sectional survey and qualitative participatory methods. Quantitative and qualitative data were generated regarding Trypanosoma cruzi reservoirs and triatomine hosts. Blood meal analysis and infection of insects, dogs and rodents were determined. Based on these data, multimodel inference was used to identify risk factors for domestic infestation with the greatest relative importance (>0.75. PRINCIPAL FINDINGS: Blood meal analysis showed that 64% of 36 bugs fed on chickens, 50% on humans, 17% on dogs; 24% of 34 bugs fed on Rattus rattus and 21% on Mus musculus. Seroprevalence among 80 dogs was 37%. Eight (17% of 46 M. musculus and three (43% of seven R. rattus from households with infected triatomines were infected with T. cruzi Distinct Typing Unit I. Results from interviews and participatory meetings indicated that vector control personnel and some householders perceived chickens roosting and laying eggs in the house as bug infestation risk factors. House construction practices were seen as a risk factor for bug and rodent infestation, with rodents being perceived as a pest by study participants. Multimodel inference showed that house infestation risk factors of high relative importance are dog density, mouse presence, interior wall plaster condition, dirt floor, tile roofing and coffee tree presence. CONCLUSIONS/SIGNIFICANCE: Persistent house infestation is closely related to eco-bio-social factors that maintain productive T. dimidiata habitats associated with dogs, chickens and rodents. Triatomine, dog and rodent infections indicate active T. cruzi transmission. Integrated vector control methods should include actions that consider the

  7. Genetic characterization of residual Triatoma infestans populations from Brazil by microsatellite.

    Science.gov (United States)

    Belisário, Carlota Josefovicz; Pessoa, Grasielle Caldas D'Avila; Silva, Eduardo Melos; Rosa, Aline Cristine Luiz; Ferreira, Rafaela Elias; Bedin, Cleonara; Wilhelms, Tania; de Mello, Fernanda; Coutinho, Helder Silveira; Fonseca, Eduardo Lins Oyama; Dos Santos, Roberto Fonseca; Rodrigues, Vera Lucia Cortiço Corrêa; Dias, João Carlos Pinto; Diotaiuti, Liléia

    2017-02-01

    In spite of long-term efforts to eliminate Triatoma infestans (Klug 1834) from Brazil, residual foci still persist in the states of Bahia and Rio Grande do Sul. Data on the genetic variability and structuring of these populations are however lacking. Using nine microsatellite loci, we characterized one residual T. infestans population from Bahia and four from Rio Grande do Sul, and compared them with bugs originally from an older focus in São Paulo; 224 bugs were genotyped. The number of alleles per locus ranged from 5 to 11. Observed and expected heterozygosities per locus ranged, respectively, from 0 to 0.786 and from 0 to 0.764. Significant departures from Hardy-Weinberg equilibrium, mainly due to heterozygote deficits, were detected in all loci and in most populations. Global indices estimated by AMOVA were: Fis was 0.37; Fst was 0.28; and Fit was 0.55; overall indices with p = 0.00 indicated substantial differentiation. Inter-population Fst ranged from 0.118 to 0.562, suggesting strong genetic structuring and little to no gene flow among populations. Intra-population Fis ranged from 0.301 to 0.307. Inbreeding was apparent in all populations except that from Bahia-which might be either linked by gene flow to nearby unsampled populations or part of a relatively large local population. The overall pattern of strong genetic structuring among pyrethroid-susceptible residual T. infestans populations suggests that their persistence is probably due to operational control failures. Detection and elimination of such residual foci is technically feasible and must become a public health priority in Brazil.

  8. Tigutcystatin, a cysteine protease inhibitor from Triatoma infestans midgut expressed in response to Trypanosoma cruzi

    Energy Technology Data Exchange (ETDEWEB)

    Buarque, Diego S.; Spindola, Leticia M.N. [Department of Biochemistry, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, 04044-020 Sao Paulo, SP (Brazil); Martins, Rafael M. [Biology of Host Parasite Interactions Unit, Institute Pasteur, 75015 Paris (France); Braz, Gloria R.C. [Department of Biochemistry, Instituto de Quimica, Universidade Federal do Rio de Janeiro, 21941-909 Rio de Janeiro (Brazil); Tanaka, Aparecida S., E-mail: Tanaka.bioq@epm.br [Department of Biochemistry, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, 04044-020 Sao Paulo, SP (Brazil)

    2011-09-23

    Highlights: {yields} Tigutcystatin inhibits Trypanosoma cruzi cysteine proteases with high specificity. {yields} Tigutcystatin expression is up-regulated in response to T. cruzi infection. {yields} It is the first cysteine proteases inhibitor characterized from a triatomine insect. -- Abstract: The insect Triatoma infestans is a vector of Trypanosoma cruzi, the etiological agent of Chagas disease. A cDNA library was constructed from T. infestans anterior midgut, and 244 clones were sequenced. Among the EST sequences, an open reading frame (ORF) with homology to a cystatin type 2 precursor was identified. Then, a 288-bp cDNA fragment encoding mature cystatin (lacking signal peptide) named Tigutcystatin was cloned fused to a N-terminal His tag in pET-14b vector, and the protein expressed in Escherichia coli strain Rosetta gami. Tigutcystatin purified and cleaved by thrombin to remove His tag presented molecular mass of 11 kDa and 10,137 Da by SDS-PAGE and MALDI-TOF mass spectrometry, respectively. Purified Tigutcystatin was shown to be a tight inhibitor towards cruzain, a T. cruzi cathepsin L-like enzyme (K{sub i} = 3.29 nM) and human cathepsin L (K{sub i} = 3.78 nM). Tissue specific expression analysis showed that Tigutcystatin was mostly expressed in anterior midgut, although amplification in small intestine was also detected by semi quantitative RT-PCR. qReal time PCR confirmed that Tigutcystatin mRNA is significantly up-regulated in anterior midgut when T. infestans is infected with T. cruzi. Together, these results indicate that Tigutcystatin may be involved in modulation of T. cruzi in intestinal tract by inhibiting parasite cysteine proteases, which represent the virulence factors of this protozoan.

  9. Evolutionary and dispersal history of Triatoma infestans, main vector of Chagas disease, by chromosomal markers.

    Science.gov (United States)

    Panzera, Francisco; Ferreiro, María J; Pita, Sebastián; Calleros, Lucía; Pérez, Ruben; Basmadjián, Yester; Guevara, Yenny; Brenière, Simone Frédérique; Panzera, Yanina

    2014-10-01

    Chagas disease, one of the most important vector-borne diseases in the Americas, is caused by Trypanosoma cruzi and transmitted to humans by insects of the subfamily Triatominae. An effective control of this disease depends on elimination of vectors through spraying with insecticides. Genetic research can help insect control programs by identifying and characterizing vector populations. In southern Latin America, Triatoma infestans is the main vector and presents two distinct lineages, known as Andean and non-Andean chromosomal groups, that are highly differentiated by the amount of heterochromatin and genome size. Analyses with nuclear and mitochondrial sequences are not conclusive about resolving the origin and spread of T. infestans. The present paper includes the analyses of karyotypes, heterochromatin distribution and chromosomal mapping of the major ribosomal cluster (45S rDNA) to specimens throughout the distribution range of this species, including pyrethroid-resistant populations. A total of 417 specimens from seven different countries were analyzed. We show an unusual wide rDNA variability related to number and chromosomal position of the ribosomal genes, never before reported in species with holocentric chromosomes. Considering the chromosomal groups previously described, the ribosomal patterns are associated with a particular geographic distribution. Our results reveal that the differentiation process between both T. infestans chromosomal groups has involved significant genomic reorganization of essential coding sequences, besides the changes in heterochromatin and genomic size previously reported. The chromosomal markers also allowed us to detect the existence of a hybrid zone occupied by individuals derived from crosses between both chromosomal groups. Our genetic studies support the hypothesis of an Andean origin for T. infestans, and suggest that pyrethroid-resistant populations from the Argentinean-Bolivian border are most likely the result of

  10. Hábito alimentar de Triatoma vitticeps no Estado do Rio de Janeiro, Brasil

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    Gonçalves Teresa Cristina M

    2000-01-01

    Full Text Available OBJETIVO: O estudo do hábito alimentar dos triatomíneos tem contribuído para o conhecimento da sua biologia no habitat natural. Triatoma vitticeps, espécie que vem invadindo freqüentemente o domicílio apresentando-se infectado por T. cruzi, foi analisado sob esse aspecto, possibilitando conhecer a situação epidemiológica da área. MÉTODOS: De fevereiro de 1989 a abril de 1993, 122 espécimes de T. vitticeps foram capturados em duas áreas da localidade de Triunfo, 2° Distrito do Município de Santa Maria Madalena (RJ. Os insetos foram dissecados para a retirada do conteúdo estomacal. Os anti-soros utilizados foram: homem, vaca, cavalo, cão, porco, tatu, gambá, roedor e ave. RESULTADOS: Do total analisado, 79 estavam positivos e 43 negativos para os anti-soros testados: tatu (30,3% > homem e porco (13,1% > ave e cão (11,5% > cavalo (5,7% > gambá (4,9% > roedor (4,1% > boi (3,3%. As fontes alimentares identificadas variaram de 1 a 4 e 6: 0 - 25,41%; 1 - 45,08%; 2 - 10,66%; 3 - 6,56%; 4 - 1,64% e 6 - 0,82%. Quanto à infecção por T. cruzi, 74 espécimes (65,54% estavam positivos, 39 (34,51% negativos e 9 não foram examinados. CONCLUSÕES: Os resultados caracterizam o hábito silvestre de T. vitticeps e a tripanosomíase como uma enzootia. A vigilância epidemiológica se faz necessária para o acompanhamento do comportamento dessa espécie.

  11. Simulating population genetics of pathogen vectors in changing landscapes: guidelines and application with Triatoma brasiliensis.

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    Francois Rebaudo

    2014-08-01

    Full Text Available Understanding the mechanisms that influence the population dynamics and spatial genetic structure of the vectors of pathogens infecting humans is a central issue in tropical epidemiology. In view of the rapid changes in the features of landscape pathogen vectors live in, this issue requires new methods that consider both natural and human systems and their interactions. In this context, individual-based model (IBM simulations represent powerful yet poorly developed approaches to explore the response of pathogen vectors in heterogeneous social-ecological systems, especially when field experiments cannot be performed.We first present guidelines for the use of a spatially explicit IBM, to simulate population genetics of pathogen vectors in changing landscapes. We then applied our model with Triatoma brasiliensis, originally restricted to sylvatic habitats and now found in peridomestic and domestic habitats, posing as the most important Trypanosoma cruzi vector in Northeastern Brazil. We focused on the effects of vector migration rate, maximum dispersal distance and attraction by domestic habitat on T. brasiliensis population dynamics and spatial genetic structure. Optimized for T. brasiliensis using field data pairwise fixation index (FST from microsatellite loci, our simulations confirmed the importance of these three variables to understand vector genetic structure at the landscape level. We then ran prospective scenarios accounting for land-use change (deforestation and urbanization, which revealed that human-induced land-use change favored higher genetic diversity among sampling points.Our work shows that mechanistic models may be useful tools to link observed patterns with processes involved in the population genetics of tropical pathogen vectors in heterogeneous social-ecological landscapes. Our hope is that our study may provide a testable and applicable modeling framework to a broad community of epidemiologists for formulating scenarios of

  12. [Morphological and histological study of eggs of six species of the Triatoma genus (Hemiptera: Reduviidae)].

    Science.gov (United States)

    Obara, Marcos T; da Rosa, João A; da Silva, Nilza N; Ceretti, Walter; Urbinatti, Paulo R; Barata, José M S; Jurberg, José; Galvão, Cleber

    2007-01-01

    The objective of this study was to analyze the morphological and histological characteristics of eggs from six triatomine species in order to obtain a generic and specific characterization of this group of Trypanosoma cruzi vectors and better understand their phylogenetic and taxonomic aspects. The eggs of the respective species came from the collection of the Laboratório de Triatomíneos e Culicídeos da Faculdade de Saúde Pública/USP, from the Insetário do Serviço Especial de Saúde de Araraquara - SESA and from the Triatomine insectary of the Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos (FIOCRUZ), in Rio de Janeiro. Morphological studies were done with optical microscopy and scanning electron microscopy. Histological analyses used only optical microscopy. All of the species analyzed showed a predominance of hexagonal cells. The exchorion of Triatoma breyeri Del Ponte, T. costalimai Verano Galvão, and T. tibiamaculata (Pinto) consists of unornamented cells, overlapping or slightly separated, with a smooth, padded appearance. Meanwhile, in T. matogrossensis Leite Barbosa, T. sherlocki Papa, Juberg, Carcavallo, Cerqueira Barata, and T. williami Galvão, Souza Lima, the exchorion cells are ornamented on their entire surface with perforations and fissures. Egg histology showed undulations, pores, and orifices. The study helps to expand the generic and specific knowledge of the Triatominae subfamily. The characteristics identified in the exchorion of the eggshells can help separate close species. These new parameters will back the elaboration of future dichotomous keys, help to determine each vector species role and expand knowledge of the various species.

  13. Monitoring the domiciliary and peridomiciliary invasion process of Triatoma rubrovaria in the State of Rio Grande do Sul, Brazil

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    Almeida Carlos Eduardo

    2000-01-01

    Full Text Available The presence of Triatoma rubrovaria in Brazil has only been confirmed in the States of Paraná and Rio Grande do Sul (RS, where it is found naturally infected with Trypanosoma cruzi. In the wild environment it occurs in rocky habitats and has an eclectic diet, feeding from cockroaches, reptiles and mammals. Data from the Chagas Disease Control Program obtained by the Fundação Nacional de Saúde, between 1975 and 1997, indicate a growing domiciliary and peridomiciliary invasion of T. rubrovaria in RS, where it has become the most frequently Triatominae species captured in this state since the control of Triatoma infestans. In order to monitor this process, we analyzed collection data derived from 22 years of control campaigns against T. infestans. Collection data for triatomines from domestic habitats show an inverse relationship, with high numbers of T. infestans and low numbers of T. rubrovaria during 1976-1987, compared to the following ten years, 1986-1997, when the number of T. infestans dropped drastically and that of T. rubrovaria increased. There are no consistent indications of intradomiciliary colonization by T. rubrovaria, since only low numbers of nymphs have been captured in the intradomiciliary ecotopes. Nevertheless, this species appears to have preadaptive characteristics for anthropic ecotopes, and should be kept under constant epidemiological surveillance.

  14. Revalidation of Triatoma bahiensis Sherlock & Serafim, 1967 (Hemiptera: Reduviidae) and phylogeny of the T. brasiliensis species complex.

    Science.gov (United States)

    Mendonça, Vagner José; Alevi, Kaio Cesar Chaboli; Pinotti, Heloisa; Gurgel-Gonçalves, Rodrigo; Pita, Sebastián; Guerra, Ana Letícia; Panzera, Francisco; De Araújo, Renato Freitas; Azeredo-Oliveir, Maria Tercília Vilela De; Rosa, João Aristeu Da

    2016-05-02

    Triatoma bahiensis Sherlock & Serafim, 1967, T. lenti Sherlock & Serafim, 1967, and T. pessoai Sherlock & Serafim, 1967 were described based on material collected in the Brazilian state of Bahia. These species were later included in the T. brasiliensis complex based on their geographic distribution. Triatoma bahiensis and T. pessoai were subsequently synonymized with T. lenti. However, the phylogenetic position of T. lenti within the T. brasiliensis complex has remained doubtful. This study aims to assess the taxonomic status of T. bahiensis and to infer the phylogenetic relationships between T. lenti, T. bahiensis and the other members of the T. brasiliensis species complex. The identities of the species in concern were confirmed by comparisons with high resolution photos of the respective type materials; lectotypes are designated for T. pessoai and T. bahiensis. Morphological, morphometric, molecular, and cytogenetic approaches as well as experimental crosses were used. The low viability of experimental crosses combined with morphological and morphometric data allow the differentiation of T. bahiensis and T. lenti. Pairwise cyt b sequence divergence between T. lenti and T. bahiensis was 2.5%. Cytogenetic and molecular analyses grouped T. lenti and T. bahiensis as members of the T. brasiliensis complex. These results revalidate the specific status of T. bahiensis.

  15. Behavioral and electrophysiological responses of Triatoma brasiliensis males to volatiles produced in the metasternal glands of females.

    Science.gov (United States)

    Vitta, Ana C R; Bohman, Björn; Unelius, C Rikard; Lorenzo, Marcelo G

    2009-10-01

    In many insects, mate finding is mediated by volatile sex pheromones, but evidence for this phenomenon in triatomines (Heteroptera: Reduviidae) is still fragmentary. Recently, it was shown that metasternal glands (MGs) are involved in producing signals related to the sexual communication of Triatoma infestans and Rhodnius prolixus. Based on this, we tested whether MG volatiles could be involved in the sexual communication of Triatoma brasiliensis. Odor-mediated orientation responses were studied by using a T-tube olfactometer. These tests showed that males exhibit positive anemotaxis when confronted with adult odor-laden air currents. Moreover, females that had their metasternal glands occluded did not elicit significant orientation by males. Compounds produced by the MGs of T. brasiliensis females were identified by means of SPME, GC-FID, and GC-MS, with achiral and chiral columns. All substances identified were ketones and alcohols, and similar compound profiles were found in the secretions produced by both sexes. The most abundant compounds identified were 3-pentanone, followed by (4R)-methyl-1-heptanol, 3-pentanol, and (2S)-methyl-1-butanol. In addition, GC-EAD recordings showed that the antennae of males responded to several of the main components of female MG secretions. Our results showed that compounds produced by the MGs of T. brasiliensis females are involved in the sexual communication of this species.

  16. MEMS AO for Planet Finding

    Science.gov (United States)

    Rao, Shanti; Wallace, J. Kent; Shao, Mike; Schmidtlin, Edouard; Levine, B. Martin; Samuele, Rocco; Lane, Benjamin; Chakrabarti, Supriya; Cook, Timothy; Hicks, Brian; Jung, Paul

    2008-01-01

    This slide presentation reviews a method for planet finding using microelectromechanical systems (MEMS) Adaptive Optics (AO). The use of a deformable mirror (DM) is described as a part of the instrument that was designed with a nulling interferometer. The strategy that is used is described in detail.

  17. Cometas: Das Lendas aos Fatos

    Science.gov (United States)

    Voelzke, M. R.

    O descobrimento de cometas, devido ao seu aparecimento espetacular, tem registro nas mais antigas culturas humanas. A primeira referência situa-se no ano de 1095 antes de Cristo [a.C.; HO; HO, 1962]. A quantidade de registros de descobrimentos cometários, principalmente provenientes do território chinês em particular e do oriente em geral, aumentou gradualmente a partir do quarto século depois de Cristo (d.C.). É de origem chinesa a primeira referência ao cometa P/Halley no ano de 240 a.C. [VOELZKE, 1993]. Com o desenvolvimento da astronomia relativamente às técnicas observacionais os descobrimentos bem como as observações cometárias aumentaram sensivelmente a partir do século XVII, sendo que a partir do século XIX um novo incremento ocorreu devido ao emprego da fotografia e a resultante melhora de sensibilidade na observação.

  18. Under-Expression of Chemosensory Genes in Domiciliary Bugs of the Chagas Disease Vector Triatoma brasiliensis

    Science.gov (United States)

    Marchant, Axelle; Mougel, Florence; Jacquin-Joly, Emmanuelle; Costa, Jane; Almeida, Carlos Eduardo; Harry, Myriam

    2016-01-01

    Background In Latin America, the bloodsucking bugs Triatominae are vectors of Trypanosoma cruzi, the parasite that causes Chagas disease. Chemical elimination programs have been launched to control Chagas disease vectors. However, the disease persists because native vectors from sylvatic habitats are able to (re)colonize houses—a process called domiciliation. Triatoma brasiliensis is one example. Because the chemosensory system allows insects to interact with their environment and plays a key role in insect adaption, we conducted a descriptive and comparative study of the chemosensory transcriptome of T. brasiliensis samples from different ecotopes. Methodology/Principal Finding In a reference transcriptome built using de novo assembly, we found transcripts encoding 27 odorant-binding proteins (OBPs), 17 chemosensory proteins (CSPs), 3 odorant receptors (ORs), 5 transient receptor potential channel (TRPs), 1 sensory neuron membrane protein (SNMPs), 25 takeout proteins, 72 cytochrome P450s, 5 gluthatione S-transferases, and 49 cuticular proteins. Using protein phylogenies, we showed that most of the OBPs and CSPs for T. brasiliensis had well supported orthologs in the kissing bug Rhodnius prolixus. We also showed a higher number of these genes within the bloodsucking bugs and more generally within all Hemipterans compared to the other species in the super-order Paraneoptera. Using both DESeq2 and EdgeR software, we performed differential expression analyses between samples of T. brasiliensis, taking into account their environment (sylvatic, peridomiciliary and domiciliary) and sex. We also searched clusters of co-expressed contigs using HTSCluster. Among differentially expressed (DE) contigs, most were under-expressed in the chemosensory organs of the domiciliary bugs compared to the other samples and in females compared to males. We clearly identified DE genes that play a role in the chemosensory system. Conclusion/Significance Chemosensory genes could be good

  19. Morfologia externa de Triatoma ryckmani Zeledón & Ponce, 1972 vista através da microscopia eletrônica de varredura External morphology of Triatoma rickmani Zeledón & Ponce, 1972 by scanning electron microscopy

    Directory of Open Access Journals (Sweden)

    Rodolfo U Carcavallo

    1996-12-01

    Full Text Available A male of Triatoma ryckmani Zeledón & Ponce, 1972, was studied by scanning electron microscopy. Only few specimens of this species are known. In this paper, some structures from the head, thorax, abdomen and distal region of the second leg are shown. Some of them could have taxonomic importance, as the oculo-ocellar region, the buccula, the anterolateral angle of the collar, the scutellum with the process longer than the main body, the stridulatory sulcus with an unusual backward vermiform area, and the tibia-tarsal articulation, with a spongy fossula. The last structure was absent in specimens previously studied (Lent & Wygodzinsky 1979. Differences between this specimen and others previously described by several authors are dicussed.

  20. Morfometria comparada de Triatoma infestans, T. rubrovaria e T. platensis (Hemiptera, Reduviidae, Triatominae do Uruguai Comparative Morphometry among Triatoma infestans, T. rubrovaria and T. platensis (Hemiptera, Reduviidae, Triatominae from Uruguay

    Directory of Open Access Journals (Sweden)

    Carolina M. dos Santos

    2009-03-01

    Full Text Available No Uruguai, são conhecidas 10 espécies de triatomíneos com importância epidemiológica variável, dentre as quais destacamse Triatoma infestans (Klug, 1834 e T. rubrovaria (Blanchard, 1843, consideradas as principais espécies vetoras. Triatoma platensis Neiva, 1913, apesar de ser uma espécie silvestre, pode conviver com T. infestans em galinheiros, chegando a gerar híbridos férteis. Estas três espécies fazem parte do "complexo T. infestans", formado por cinco espécies. Devido à importância epidemiológica dessas espécies no Uruguai e com a finalidade de ampliar o conjunto dos parâmetros morfológicos diferenciais destas espécies apresentamos uma abordagem morfométrica comparativa da cabeça e do pronoto. Foram medidas quatro variáveis da cabeça e quatro do pronoto a partir de 50 machos e 50 fêmeas, de cada espécie, provenientes de criação de laboratório. O programa JMP foi utilizado para as análises de Componentes Principais (ACP e Discriminante (AD. Os resultados demonstraram que os dois primeiros componentes principais (CP1 e CP2 exibem aproximadamente 77% da variação total. Os caracteres mais importantes para a distinção das espécies foram a região anteocular (RAO e a região pós-ocular (RPO, indicando que a morfometria da cabeça se mostrou mais eficiente que o pronoto para diferenciar estas espécies. A análise discriminante possibilitou separar T. platensis e T. rubrovaria mas não foi capaz de separá-las de T. infestans.In Uruguay, we can find 10 species with different epidemiological importance, amongst them we emphasize Triatoma infestans (Klug, 1834 and T. rubrovaria (Blanchard, 1843, considered main vectors. Triatoma platensis Neiva, 1913, that although being a wild species can cohabit with T. infestans in chicken coops. These three species, belong to "T. infestans complex", wich is composed by five species. Because the epidemiological importance of these species in Uruguay and, with the aim to

  1. Sobrevivência e ocorrência de muda em Triatoma infestans Klug (Hemiptera, Reduviidae após choque de temperatura Survival and molting occurrence in Triatoma infestans Klug (Hemiptera, Reduviidae after temperature shocks

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    Vera Lúcia C.C. Rodrigues

    1991-12-01

    Full Text Available A sobrevivência e a ocorrência de mudas em espécimes de Triatoma infestans foram estudados num período de 30 dias após choques de temperatura. Foi demonstrado que choques hipertérmico e hipotérmico interferem nesses processos, na dependência da temperatura do choque, tempo de sua duração, fase de desenvolvimento e sexo dos espécimes. Dentre as situações experimentais utilizadas, o choque a 0°C por 12 h pareceu produzir a ação mais deletéria, porém choques a 40°C e 0°C mesmo por uma hora são admitidos como afetando o desenvolvimento hormonal que controla a muda. Casos de aumento de sobrevivência pós-choque são sugeridos como tendo sido favorecidos por ação de proteínas de choque térmico.Survival and molting occurrence were studied in specimens of Triatoma infestans over 30 days after temperature shocks. Hyperthermal and hypotermal shocks could be found to affect both survival and molting incidence as a function of temperature and period of the developement phase and sex of the specimens. Considering the various test conditions, the shock at 0°C for 12 h was found to elicit the most deleterious effect, whereas shocks at 40°C and 0°C even for 1 h are interpreted as affecting the hormonal balance which controls molting. Cases of a rise in post-shock survival are suggested to have been favored by heat-shock protein action.

  2. Combate ao niilismo e ao totalitarismo em Camus

    Directory of Open Access Journals (Sweden)

    Emanuel Ricardo Germano

    2013-05-01

    Full Text Available A partir de uma leitura da peça Calígula, elaborada entre 1941 e 1943, procurar-se-á contribuir na restituição das preocupações éticas, políticas e filosóficas em cena no teatro de Albert Camus, analisando o sentido de sua expressão teatral no contexto de dois combates,ao niilismo filosófico e ao totalitarismo político de seu tempo. Observaremos em Calígula um importante registro do amadurecimento das leituras de duas heranças filosóficas relevantes na formação intelectual de Camus, Nietzsche e Heráclito, à luz da experiência do totalitarismo, observando e ressaltando com isso a amplitude de seu intertexto filosófico e a importância do aporte ético e político de Camus como pensador multifacetado, engajado nos enfrentamentos históricos e solicitações críticas de seu tempo.

  3. [Triatoma vandae sp.n. of the oliveirai complex from the State of Mato Grosso, Brazil (Hemiptera: Reduviidae: Triatominae)].

    Science.gov (United States)

    Carcavallo, Rodolfo U; Jurberg, José; Rocha, Dayse da Silva; Galvao, Cleber; Noireau, François; Lent, Herman

    2002-07-01

    There are several specific complexes belonging to the genus Triatoma Laporte, 1832, which are generally associated to specific geographic areas. Recent publications have linked the oliveirai complex to ecosystems of Mato Grosso, which are also present in other Brazilian states and even in other bordering countries as eastern Paraguay. The study of the abundant material collected during the last years allowed the description of several new species of the oliveirai complex: T. jurbergi Carcavallo, Galvão Lent, 1998; T. baratai Carcavallo Jurberg, 2000 and T. klugi Carcavallo, Jurberg, Lent Galvão, 2001. Another new species belonging to the same complex is described here as T. vandae sp.n. It originates from the state of Mato Grosso, and has been reared in the insectary of the Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Departamento de Entomologia, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro.

  4. [Feeding pattern of Triatoma rubrovaria (Blanchard, 1843) (Hemiptera, Triatominae) in peridomiciliary habitats, of a rural area of Uruguay].

    Science.gov (United States)

    Salvatella, R; Calegari, L; Puime, A; Basmadjian, Y; Rosa, R; Guerrero, J; Martinez, M; Mendaro, G; Briano, D; Montero, C

    1994-01-01

    It has been studied the feeding patterns of Triatoma rubrovaria (wild triatominae bug, and intradomiciliary secondary vector of T. cruzi) in a rural area of "La Bolsa" (Department of Artigas). Agar double diffusion test were used for analyze the blood meal, from 120 insects, which were confronted to 13 antisera. It has 251 identify blood meals, and the most frequently was mammalian host (73%), but it has a variable source of feeding (mammals, birds, reptiles and cockroach), including humans rate 8% of the total. The identify of haemolimphe as feed, place this triatominae bug as an intermediary vector between predator and haematophagous. The most usual mammals are dasypodides and bovines. The frequence of human blood meal near the dwellings, shows its potentiality as vectors. Therefore, the peridomicile is an area of interaction between, domestic, wild and sinantropic host. The trypanosomic infection's rate at peridomiciles was lesser those at wild areas.

  5. Susceptibility and resistance to deltamethrin of wild and domestic populations of Triatoma infestans (Reduviidae: Triatominae in Bolivia: new discoveries

    Directory of Open Access Journals (Sweden)

    Stéphanie Depickère

    2012-12-01

    Full Text Available Bolivia is a high-endemic country for Chagas disease, for which the principal vector is Triatoma infestans (Triatominae. This is a mainly domestic species that is also found in the wild environment. Recently, an increasing number of studies have shown the importance of Triatominae resistance to insecticides, especially in Bolivia. Data regarding the susceptibility/resistance of wild and domestic populations of T. infestans to deltamethrin are presented. For the first time, domestic populations of the department of Santa Cruz were tested, showing low resistance. Although most of the wild populations were found to be susceptible to deltamethrin, three populations from three departments showed a mortality rate of less than 100%. This result is emphasised here.

  6. Eye colour as a genetic marker for fertility and fecundity of Triatoma infestans (Klug, 1834 Hemiptera, Reduviidae, Triatominae

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    Pires Herton Helder Rocha

    2002-01-01

    Full Text Available Eye colour of Triatoma infestans is controlled at a single autosomal locus, with black-eye as the dominant gene and red-eye as the recessive. Inheritance of these characters follows a classical Mendelian system, enabling eye colour to be used as a marker for studies of mating frequency. We found no significant differences in oviposition rates and egg hatching rates irrespective of parental phenotypes. Different mating schedules between red-eye and black-eye parents showed that eye colour did not affect mating competence. Females mated with a single male or with different males together or in succession produced similar numbers of fertile eggs, with the eye colour of the offspring reflecting exposure to the different males. We conclude that although a single mating can provide sufficient sperm for the whole reproductive life of the female, multiple matings can result in balanced assortative sperm usage from the spermatheca.

  7. Impact of residual spraying on Rhodnius prolixus and Triatoma dimidiata in the department of Zacapa in Guatemala

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    J Nakagawa

    2003-03-01

    Full Text Available As a vector control program to control Chagas disease in Guatemala, residual spraying of Rhodnius prolixus and Triatoma dimidiata was performed, and its impact was measured in the department of Zacapa. In order to identify infested villages and determine the degree of infestation, a baseline entomological survey to identify municipalities infested with vectors followed by an additional vector survey in areas known to be infested was conducted. Residual spraying using pyrethroid insecticides was performed at all the villages identified as being infested with the vectors. The residual spraying was shown to be highly effective against both vectors by the decrease in infestation indices after spraying. Analysis of the cost-effectiveness of the spraying showed that the average cost of insecticides per house is high when compared with that in Southern Cone countries.

  8. Mitochondrial DNA variation of Triatoma infestans populations and its implication on the specific status of T. melanosoma

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    Fernando A Monteiro

    1999-09-01

    Full Text Available DNA sequence comparison of 412 base-pairs fragments of the mitochondrial cytochrome B gene was used to infer the genetic structure of nine geographical Triatoma infestans populations and their phylogenetic relationship with T. melanosoma and T. brasiliensis. T. infestans and T. melanosoma were compared by morphometry, allozyme and cytogenetic analyses, as well as subjected to reciprocal crosses, in order to clarify the taxonomic status of the latter. No differences were found to distinguish the two species and the crosses between them yielded progeny. T. infestans populations presented four haplotypes that could be separated in two clusters: one formed by the samples from Bolivia (Andes and Chaco and the other formed by samples from Argentina and Brazil. Silvatic and domestic T. infestans populations from Bolivia (Andes were genetically identical.

  9. Effect of feeding frequency on the reproductive efficiency of two species of Triatoma with different epidemiological importance

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    Claudia Rodríguez

    2014-07-01

    Full Text Available Introduction In Triatominae, reproductive efficiency is an important factor influencing population dynamics, and a useful parameter in measuring a species' epidemiological significance as a vector of Trypanosoma cruzi (Chagas, 1909. The reproductive efficiency of triatomines is affected by food availability; hence, we measured and compared the effects of feeding frequency on the reproductive parameters of Triatoma patagonica (Del Ponte, 1929 and Triatoma infestans (Klug, 1934, and the effects of starvation on T. patagonica. Methods Couples from both species were fed weekly, or every 3 weeks; in addition, females in couples of T. patagonica were not fed. Each couple was observed weekly and reproductive efficiency was assessed on the following parameters: fecundity (eggs/female, fertility (eggs hatched/eggs laid, initiation and end of oviposition, initiation of mating, number of matings/week, and number of reproductive weeks. Relative meal size index (RMS, blood consumption index (CI, and E values (eggs/mg blood were also calculated. Results Changes in feeding frequency affected the reproductive parameters of T. patagonica only, with a decrease in fecundity and number of reproductive weeks for those fed every 3 weeks, or not fed. The reproductive period, RMS index, and CI were lower for T. patagonica than T. infestans. However, despite the lower fecundity of T. patagonica, this species required less blood to produce eggs, with an E values of 2 compared to 2.94 for T. infestans. Conclusions Our results suggest that the differences in fecundity observed between species reflect the availability of food in their natural ecotopes.

  10. [Indicators of infestation, colonization and infection of Triatoma dimidiata (Latreille) (Hemiptera: Reduviidae) in Campeche, México].

    Science.gov (United States)

    Hernández, Jorge L; Rebollar-Téllez, Eduardo A; Infante, Francisco; Morón, Alejandro; Castillo, Alfredo

    2010-01-01

    Triatoma dimidiata (Latreille) is considered to be one of the primary vectors of Chagas disease in Southern Mexico and Central America. The objective of the present study was to obtain ecological information on T. dimidiata in two rural communities of Campeche, Mexico, where the vector is poorly studied. Our work consisted of monthly samplings carried out during one-year time at three levels: sylvatic, peridomestic and intradomestic, in order to estimate the population abundance of this species and its rate of infection with Trypanosoma cruzi. Triatoma dimidiata was the unique vector of this disease collected in San Juan Bautista Sakcabchen (SJBS) and Crucero San Luis (CSL). The total of 145 individuals were captured in SJBS; from these, 26.9%, 20% and 53.1% were collected in the sylvatic, peridomestic and intradomestic area, respectively. In CSL captures yielded 108 individuals: 40.7% in the sylvatic area, 20.4% peridomestic and 38.9% intradomestic. We found no correlation between climatic variables and population abundance of T. dimidiata. Dataset obtained suggests that individuals from the sylvatic area exhibit a high rate of natural infection by T. cruzi, with monthly percentages up to 61.5% for SJBS and 50% for CSL. At the peri and intradomestic level, the reservoirs apparently play an important role in the transmission, as the seroprevalence in dogs was 61.5% y 65.4%, for SJBS y CSL, respectively. Based on these findings, it was concluded that inhabitants of both communities are at a high risk of Chagas disease infection.

  11. Adrenoleucodistrofia cerebral ligada ao x

    OpenAIRE

    Ono,Sergio Eiji

    2013-01-01

    Resumo: OBJETIVO: o padrão-ouro em exames de imagem para avaliar a adrenoleucodistrofia ligada ao X é o escore de Loes, baseado na localização, alterações de sinais e grau de atrofia. Os objetivos deste estudo foram avaliar como os parâmetros do tensor de difusão correlacionam com o escore de Loes e avaliar se poderiam indicar alterações estruturais precoces. PACIENTES E MÉTODOS: as medidas do tensor de difusão foram obtidas em 9 locais pré-estabelecidos da substância branca, bilateralmente, ...

  12. Educaçao ambiental

    OpenAIRE

    Carneiro,Danielle

    2013-01-01

    Resumo: A idéia desse trabalho surgiu no momento em que me inseri na comunidade de Pontal do Sul, envolvida em distintas atividades educativas com o mesmo público. Uma delas refere-se ao ensino público fundamental e outra à atividades de pesquisa e Educação Ambiental (EA) dentro de um projeto de extensão universitária. A EA vem sendo realizada de forma diversificada, baseada em diferentes concepções, fundamentadas em princípios e diretrizes construídos num momento de difusão da Ecologia e de ...

  13. Hunting, Swimming, and Worshiping: Human Cultural Practices Illuminate the Blood Meal Sources of Cave Dwelling Chagas Vectors (Triatoma dimidiata) in Guatemala and Belize

    OpenAIRE

    Lori Stevens; M Carlota Monroy; Antonieta Guadalupe Rodas; Dorn, Patricia L.

    2014-01-01

    Background Triatoma dimidiata, currently the major Central American vector of Trypanosoma cruzi, the parasite that causes Chagas disease, inhabits caves throughout the region. This research investigates the possibility that cave dwelling T. dimidiata might transmit the parasite to humans and links the blood meal sources of cave vectors to cultural practices that differ among locations. Methodology/Principal Findings We determined the blood meal sources of twenty-four T. dimidiata collected fr...

  14. Como responder ao momento presente?

    Directory of Open Access Journals (Sweden)

    Maria Filomena Molder

    2013-06-01

    Full Text Available Foi com esta pergunta — já um efeito de um primeiro encontro entre Irene Pimentel e eu própria — que decidimos desafiar colegas, estudantes e funci­onários da nossa Faculdade, FCSH (Faculdade de Ciências Sociais e Huma­nas, de outras Faculdades da Universidade Nova de Lisboa, de outras Uni­versidades e todos os interessados em con­siderar e discutir em comum aquilo que se passava em Portugal e que no anúncio da Jornada de 6 de De­zembro de 2012 se descrevia como um “processo de desmantela­mento social, económico e cultural sem precedentes — pese embora tantas compara­ções, baseadas na premissa da ‘eterna repetição’ — e cujas consequências não param de exceder as previsões dos responsáveis por esse desmantelamento”. Acedendo com todo o empenho e gratidão ao convite que me foi dirigido por Humberto Brito para fazer uma resenha da Jornada a publicar no primeiro número de Forma de Vida (saúdo a revista e o título, decidi-me, no entanto, a pôr de lado a resenha, que sob a forma de “Editorial” será em breve publi­cada no blogue Responder ao Momento Presente, entre­tanto criado, conjuntamente com os textos escritos pelos nossos convidados, com as parti­cipações de pessoas que corresponderam ao nosso apelo e ainda com contri­bui­ções que se alargaram para lá da Jornada; a que se juntará uma gravação em video, também disponível no Youtube. Texto publicado originalmente em Forma de Vida, Lisboa, n.1, fev. 2013. Agrade­cemos à autora por permitir a republicação neste número do Boletim. [N.E.

  15. Aerosol Observing System (AOS) Handbook

    Energy Technology Data Exchange (ETDEWEB)

    Jefferson, A

    2011-01-17

    The Aerosol Observing System (AOS) is a suite of in situ surface measurements of aerosol optical and cloud-forming properties. The instruments measure aerosol properties that influence the earth’s radiative balance. The primary optical measurements are those of the aerosol scattering and absorption coefficients as a function of particle size and radiation wavelength and cloud condensation nuclei (CCN) measurements as a function of percent supersaturation. Additional measurements include those of the particle number concentration and scattering hygroscopic growth. Aerosol optical measurements are useful for calculating parameters used in radiative forcing calculations such as the aerosol single-scattering albedo, asymmetry parameter, mass scattering efficiency, and hygroscopic growth. CCN measurements are important in cloud microphysical models to predict droplet formation.

  16. Estudos anatômicos e histológicos sôbre a subfamília Triatominae (Heteroptera, Reduviidae: V parte: anatomia do testículo e espermiocitogênese do Triatoma infestans

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    Rudolf Barth

    1956-06-01

    Full Text Available Neste trabalho, faz-se o estudo das estruturas anatômica e histológica do testículo de Triatoma infestans. Da espermiogênese, descrevem-se apenas as fases que medeiam entre a formação dos espermiogônios e a dos espermídeos (espermiocitogênese. A espermiohistogênese bem como as anatomias do vas deferens e das glândulas anexas serão tratados na segunda parte dêste trabalho, já em preparo. O testículo de Triatoma infestans possui 7 folículos dos quais cada um se abre num vas efferens próprio, curto, desembocando êstes num único vas deferens geral. Na zona de transição entre vas efferens e vas deferens, encontra-se sempre um conjunto de massas tissulares que se estão necrosando em virtude da decomposição das paredes dos cistos. Em conseqüência, os feixes de espérmios são libertados e passam através do vas efferens para o vas deferens. As substâncias líquidas que então se formam, resultado da necrose, são reabsorvidas pelo epitélio do vas efferens, entrando novamente em circulação na hemolinfa; o epitélio possui um rabdório muito longo. A parte superior do conteúdo de cada folículo dispõe-se ao redor de grande célula apical cuja função principal deve ser a de uma atividade reguladora que está relacionada com a diferenciação das células do conjunto germinativo em espermiogônios primários e em núcleos das paredes dos cistos. Nos espermiogônios, serão verificadas 8 divisões de multiplicação, o que vai dar a formação de 256 espermiócitos, número êsse que depois das duas divisões de maturação, que se seguem, originará 1 024 espermídeos. Em seguida, são descritos os fenômenos que ocorrem durante a prófase e as duas divisões de maturação. Temos que admitir a existência de uma parasíndese. Pela formação das tétrades, pode-se concluir que a primeira divisão é reducional e a segunda equacional, existindo, pois, uma pré-redução. Triatoma infestans possui 22 cromosomas no espermiog

  17. La aerotermia como alternativa para el control de Triatoma infestans (Hemiptera, Reduviidae resistentes a deltametrina Air temperature elevation as an alternative for the control of deltamethrin-resistant Triatoma infestans (Hemiptera, Reduviidae

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    Alberto G. Gentile

    2004-08-01

    Full Text Available Se exploraron los efectos de la elevación artificial de la temperatura del aire sobre las formas embrionarias, ninfales y adultas de Triatoma infestansresistentes a Deltametrina. En laboratorio, se expuso a temperaturas crecientes del aire la progenie completa de T. infestans, para determinar viabilidad de huevos y supervivencia en ninfas y adultos; sobre el terreno, la experimentación se llevó a cabo en una vivienda tipo rancho previamente evaluada como colonizada. Durante la fase de laboratorio se comprobó la inviabilidad del 100% de los huevos y la mortalidad del 100% de las ninfas y adultos expuestos a temperaturas del aire estanco de entre 45ºC y 55ºC, mientras que en la fase de terreno se logró reproducir el efecto ovicida del calor, como así también la negativización de las evaluaciones entomológicas de la vivienda de experimentación, llevadas a cabo a las 72 horas, a los 30, 60 y 90 días. La elevación artificial de la temperatura del aire estanco en las habitaciones resultó ser un método de control de eficacia aceptable, inocuo y técnicamente sencillo.This study explores the effects of artificial air temperature elevation on the embryonic, nymphal, and adult forms of deltamethrin-resistant Triatoma infestans. In the laboratory, complete offspring of T. infestanswere exposed to increasing air temperatures to determine the viability of eggs and survival of nymphs and adults; in the field, the experiment was conducted in a poor rural dwelling previously identified as infested with the vector. The laboratory phase showed 100% non-viability of eggs and 100% mortality of nymphs and adults exposed to airtight temperatures from 45ºC to 55ºC, while the field phase succeeded in reproducing the heat's ovicidal effect and negative conversion of entomological assays in the experimental dwelling, as conducted over the course of 72 hours at 30, 60, and 90 days from the baseline evaluation. Artificial elevation of airtight temperature

  18. Domiciliation of Triatoma pseudomaculata (Corrêa e Espínola 1964 in the Jequitinhonha Valley, State of Minas Gerais Domiciliação de Triatoma pseudomaculata (Corrêa e Espínola 1964 no Vale do Jequitinhonha, Estado de Minas Gerais

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    Girley Francisco Machado de Assis

    2007-08-01

    Full Text Available Impact of the vector control program was evaluated eight years after implantation of epidemiological surveillance for Chagas’ disease in Berilo, a municipality in the Jequitinhonha Valley of the Brazilian State of Minas Gerais. In all 5,242 domiciliary units (96% of the total were inspected and 10 found to be infested by the triatomine bug Triatoma pseudomaculata. Triatomines were found associated with bats inside one house and in the peridomiciles of the other nine. None of the 111 Triatoma pseudomaculata captured was infected with Trypanosoma cruzi. Noireau et al16 traps were installed in (n=8 and around (n=100 the infested house but no Trypanpsoma cruzi-infected triatomines were found. None bat, opossums (Didelphis albiventris and rat captured in the peridomicile were infected with Trypanosoma cruzi although 24% of the inhabitants of the house infested by Triatoma pseudomaculata were seropositive for the parasite, based on ELISA, IHA and IIF.Oito anos após a implantação da vigilância epidemiológica para doença de Chagas em Berilo, Vale do Jequitinhonha, MG, Brasil, foi realizada uma pesquisa para verificar o impacto do Programa de Controle Vetorial. Neste trabalho, 5. 242 (96% unidades domiciliares foram vistoriadas. Dez estavam infestadas por Triatoma pseudomaculata. Em nove delas os insetos estavam infestando o peridomicílio e em uma casa foi constatado um foco intradomiciliar associado a morcegos. Foram capturados 111 insetos da espécie Triatoma pseudomaculata e nenhum exemplar estava infectado por Trypanosoma cruzi. Na casa infestada e em torno dela foram instaladas respectivamente 8 e 100 armadilhas de Noireau et al16 e nenhum triatomíneo foi capturado. Oitenta morcegos capturados e examinados também estavam negativos para Trypanosoma cruzi bem como três gambás (Didelphis albiventris e um roedor, todos capturados no peridomicílio. Um porcentual de 24% dos moradores das casas infestadas por Triatoma pseudomaculata foi

  19. SRAO: the first southern robotic AO system

    Science.gov (United States)

    Law, Nicholas M.; Ziegler, Carl; Tokovinin, Andrei

    2016-08-01

    We present plans for SRAO, the first Southern Robotic AO system. SRAO will use AO-assisted speckle imaging and Robo-AO-heritage high efficiency observing to confirm and characterize thousands of planet candidates produced by major new transit surveys like TESS, and is the first AO system to be capable of building a comprehensive several-thousand-target multiplicity survey at sub-AU scales across the main sequence. We will also describe results from Robo-AO, the first robotic LGS-AO system. Robo-AO has observed tens of thousands of Northern targets, often using a similar speckle or Lucky-Imaging assisted mode. SRAO will be a moderate-order natural-guide-star adaptive optics system which uses an innovative photoncounting wavefront sensor and EMCCD speckle-imaging camera to guide on faint stars with the 4.1m SOAR telescope. The system will produce diffraction-limited imaging in the NIR on targets as faint as mν = 16. In AO-assisted speckle imaging mode the system will attain the 30-mas visible diffraction limit on targets at least as faint as mν = 17. The system will be the first Southern hemisphere robotic adaptive optics system, with overheads an order of magnitude smaller than comparable systems. Using Robo-AO's proven robotic AO software, SRAO will be capable of observing overheads on sub-minute scales, allowing the observation of at least 200 targets per night. SRAO will attain three times the angular resolution of the Palomar Robo-AO system in the visible.

  20. MagAO: status and science

    Science.gov (United States)

    Morzinski, Katie M.; Close, Laird M.; Males, Jared R.; Hinz, Phil M.; Esposito, Simone; Riccardi, Armando; Briguglio, Runa; Follette, Katherine B.; Pinna, Enrico; Puglisi, Alfio; Vezilj, Jennifer; Xompero, Marco; Wu, Ya-Lin

    2016-07-01

    "MagAO" is the adaptive optics instrument at the Magellan Clay telescope at Las Campanas Observatory, Chile. MagAO has a 585-actuator adaptive secondary mirror and 1000-Hz pyramid wavefront sensor, operating on natural guide stars from R-magnitudes of -1 to 15. MagAO has been in on-sky operation for 166 nights since installation in 2012. MagAO's unique capabilities are simultaneous imaging in the visible and infrared with VisAO and Clio, excellent performance at an excellent site, and a lean operations model. Science results from MagAO include the first ground-based CCD image of an exoplanet, demonstration of the first accreting protoplanets, discovery of a new wide-orbit exoplanet, and the first empirical bolometric luminosity of an exoplanet. We describe the status, report the AO performance, and summarize the science results. New developments reported here include color corrections on red guide stars for the wavefront sensor; a new field stop stage to facilitate VisAO imaging of extended sources; and eyepiece observing at the visible-light diffraction limit of a 6.5-m telescope. We also discuss a recent hose failure that led to a glycol coolant leak, and the recovery of the adaptive secondary mirror (ASM) after this recent (Feb. 2016) incident.

  1. MagAO: Status and Science

    CERN Document Server

    Morzinski, Katie M; Males, Jared R; Hinz, Phil M; Esposito, Simone; Riccardi, Armando; Briguglio, Runa; Follette, Katherine B; Pinna, Enrico; Puglisi, Alfio; Vezilj, Jennifer; Xompero, Marco; Wu, Ya-Lin

    2016-01-01

    "MagAO" is the adaptive optics instrument at the Magellan Clay telescope at Las Campanas Observatory, Chile. MagAO has a 585-actuator adaptive secondary mirror and 1000-Hz pyramid wavefront sensor, operating on natural guide stars from $R$-magnitudes of -1 to 15. MagAO has been in on-sky operation for 166 nights since installation in 2012. MagAO's unique capabilities are simultaneous imaging in the visible and infrared with VisAO and Clio, excellent performance at an excellent site, and a lean operations model. Science results from MagAO include the first ground-based CCD image of an exoplanet, demonstration of the first accreting protoplanets, discovery of a new wide-orbit exoplanet, and the first empirical bolometric luminosity of an exoplanet. We describe the status, report the AO performance, and summarize the science results. New developments reported here include color corrections on red guide stars for the wavefront sensor, a new field stop stage to facilitate VisAO imaging of extended sources; and eye...

  2. A Triatoma maculata (Hemiptera, Reduviidae, Triatominae population from Roraima, Amazon region, Brazil, has some bionomic characteristics of a potential Chagas disease vector Uma população de Triatoma maculata (Hemiptera, Reduviidae, Triatominae proveniente de Roraima, Amazônia, Brasil, possui algumas características bionômicas de vetor potencial de doença de Chagas

    Directory of Open Access Journals (Sweden)

    José Francisco Luitgards-Moura

    2005-06-01

    transmissão virem a ocorrer uma vez que todos os elementos estavam lá presentes, indivíduos infectados por Trypanosoma cruzi, espécies de triatomíneos anteriormente descritas como infectadas por T. cruzi na Região Amazônica de países fronteiriços e, ambientes domiciliares e peri-domiciliares favoráveis à colonização de triatomíneos. Triatoma maculata foi a espécie mais frequentemente encontrada, tendo sido coletada em galinheiros no peridomicílio e esporadicamente nos domicílios. Visando investigar a potencialidade de T. maculata como espécie vetora na área, algumas características bionômicas foram estudadas em condições de laboratório incluindo freqüência de alimentação, tempo de defecação pós-prandial, tempo de jejum voluntário na pré- e na pós-ecdise, período inter-mudas, períodos de pré-oviposição e de oviposição, índice de oviposição, período de incubação, viabilidade dos ovos, índices de longevidade e de mortalidade. Os resultados mostraram que a população de T. maculata da Colônia Agrícola do Passarão deve ser considerada vetora em potencial do T. cruzi uma vez que mostrou capacidade de infestar ecótopos artificiais no peridomicílio, de se alimentar com freqüência durante o período ninfal, de possuir um ciclo de desenvolvimento relativamente curto com 2,9 gerações/ano, de possuir hábitos ecléticos de alimentação, de defecar imediatamente após a hematofagia quando ainda no hospedeiro e devido ao fato de ter sido previamente encontrada infectada por T. cruzi.

  3. Soroprevalência de infecção chagásica em área de Triatoma infestans após medidas de controle Seroprevalence of Chagas disease in an area of Triatoma infestans after vector control measures

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    Maria Esther Carvalho

    2000-02-01

    Full Text Available INTRODUÇÃO: Como parte de projeto que busca resgatar informações sobre sorologia da infecção chagásica no Estado de São Paulo durante a vigência das atividades oficiais de controle de vetores, foram selecionados dados relativos ao município de Taquarituba, região de Sorocaba, de importante passado chagásico. A despeito da campanha de combate instituída na década de 50, ainda persistiam aí populações de triatomíneos vetores intradomiciliares na década de 70. MÉTODOS: De amostras das populações de cinco localidades do Município de Taquarituba, Estado de São Paulo, foram analisados os dados de idade, sexo, tempo de moradia na casa que ocupavam e naturalidade. A possível relação entre idade e soropositividade foi investigada utilizando-se o cálculo de probitos. De 1974 e 1976, a prevalência da infecção chagásica foi estimada por meio de reação de imunofluorescência indireta (RIFI para a detecção de anticorpos anti-Trypanosoma cruzi. RESULTADOS: A proporção total de soropositivos foi de 13,6% (n = 2.784, sem diferença significativa entre os sexos (p = 0,538. Os naturais de Taquarituba contribuíram com 62,9% das amostras examinadas e 62,4% dos soropositivos (n = 380. Ausente em crianças de menos de 6 anos, a soropositividade aumentou de 2,7% no grupo etário de 6 a 9 anos para 30,3% no de 30 a 39 anos. Dentro desse intervalo encontrou-se, por meio do cálculo de probitos, associação positiva entre idade e soropositividade. CONCLUSÕES: Os resultados das provas sorológicas sugeriram haver associação entre as medidas contra Triatoma infestans e o declínio da transmissão da doença de Chagas durante os anos 60, seguido de ulterior controle. Sugere-se a realização de pesquisa para investigar a ocorrência de infecção congênita em população feminina com mais de 14 anos, sorologicamente positivas.INTRODUCTION: The study is part of a project intended to retrieve information about the serology of the

  4. Susceptibilidad y resistencia de Triatoma infestans y Panstrongylus herreri a los insecticidas piretroides, Perú 2001

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    Carmen Yon F

    2004-07-01

    Full Text Available Se evaluaron los niveles de susceptibilidad de triatominos a piretroides con la técnica de la OMS, usando papeles impregnados al 0,1% de ingrediente activo de los siguientes insecticidas: alfacipermetrina, betaciflutrina, ciflutrina, cipermetrina y deltametrina, se emplearon lotes de triatominos de los estadios ninfa V y adultos de Triatoma infestans y Panstrongylus herreri. Se registraron los porcentajes de mortalidad después de las 24 horas de exposición a papeles impregnados con insecticidas, observando que la mayor parte de la población de triatominos de ambas especies era sensibles a los insecticidas. Sólo en algunos casos, se hallaron los niveles de resistencia en los estadios de ninfa V en la especie P. herreri en 2 localidades de la provincia de Jaén, departamento de Cajamarca, asimismo en adultos y ninfas V de T. infestans en especímenes capturados en el valle de Vítor, departamento de Arequipa.

  5. Evaluación del efecto insecticida de tierra de diatomeas sobre Triatoma infestans (Hemíptera, Reduviidae

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    Mariana L Bilbao

    2007-04-01

    Full Text Available El Triatoma infestans es el principal vector de la enfermedad de Chagas en Argentina. La tierra de diatomeas (TD es un producto natural que se comercializa actualmente con fines insecticidas y que produce la muerte por deshidratación. Se evaluó la eficacia insecticida de TD al 60% de pureza, proveniente del yacimiento La Iglesiana (San Juan, Argentina sobre ninfas V y adultos de T.infestans. Se ensayaron dos dosis: 112,5 g/m2 (T1 y 225 g/m2 (T2, con cuatro réplicas por tratamiento, con cinco ejemplares, incluyendo los controles (TC en el laboratorio. Se contaron los individuos vivos y muertos durante cinco días. No se encontró diferencias en la mortalidad (ANOVA, p>0,05 en los adultos (T1: 40%, T2: 25% y TC: 30% ni en las ninfas V (T1: 10%, T2: 15% y TC: 10%. Se concluye que la tierra de diatomeas del yacimiento La Iglesiana, en las condiciones de laboratorio evaluadas, no posee capacidad insecticida sobre T.infestans.

  6. Influence of temperature and humidity on the biology of Triatoma mexicana (Hemiptera: Reduviidae: Triatominae under laboratory conditions

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    José Alejandro Martínez-Ibarra

    2008-11-01

    Full Text Available Several biological parameters related to the Triatoma mexicana life-cycle were evaluated in this study. Three cohorts were maintained under different combinations of temperature and relative humidity (RH: 25ºC/50% RH; 25ºC/75% RH; and 30ºC/75% RH. Observed hatching rates varied from 49-57.5% whereas the average time of hatching varied from 19.5-22.7 days. In the three cohorts studied, the mean time-lapse between presentation of the blood meal and the beginning of feeding was less than 5 min in all instars; the mean feeding time was longer than 10 min in all the instars; the post-feed defecation delay was over 10 min in all the instars. Less than 50% of nymphs in each cohort completed the cycle and the average time from 1st instar nymph to adult was more than 255 days for the three cohorts. The number of blood meals before molt at each nymphal instar varied from 1-9. Our results appear to indicate a lack of influence of temperature and RH on the biological parameters of T. mexicana that were studied, which could reflect the adaptation capacity of this species. We also conclude that T. mexicana can not be considered an effective transmitter of Trypanosoma cruzi to human populations in areas where this species is currently present.

  7. Effects of Non-Susceptible Hosts on the Infection with Trypanosoma cruzi of the Vector Triatoma infestans: an Experimental Model

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    Vázquez Diego P

    1999-01-01

    Full Text Available We tested experimentally the effects of the presence of non-susceptible hosts on the infection with Trypanosoma cruzi of the vector Triatoma infestans. The experiment consisted in two treatments: with chickens, including two chickens (non-susceptible hosts and two infected guinea pigs (susceptible hosts, and without chickens, including only two infected guinea pigs. The hosts were held unrestrained in individual metal cages inside a closed tulle chamber. A total of 200 uninfected T. infestans third instar nymphs were liberated in each replica, collected on day 14, and examined for infection and blood meal sources on day 32-36. The additional presence of chickens relative to infected guinea pigs: (a significantly modified the spatial distribution of bugs; (b increased significantly the likelihoods of having a detectable blood meal on any host and molting to the next instar; (c did not affect the bugs' probability of death by predation; and (d decreased significantly the overall percentage of T. infestans infected with T. cruzi. The bugs collected from inside or close to the guinea pigs' cages showed a higher infection rate (71-88% than those collected from the chickens' cages (22-32%. Mixed blood meals on chickens and guinea pigs were detected in 12-21% of bugs. Although the presence of chickens would decrease the overall percentage of infected bugs in short term experiments, the high rate of host change of T. infestans would make this difference fade out if longer exposure times had been provided.

  8. Potential sources of Triatoma infestans reinfesting peridomiciles identified by morphological characterization in Los Llanos, La Rioja, Argentina

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    María Laura Hernández

    2013-02-01

    Full Text Available The presence of Triatoma infestans in habitats treated with insecticides constitutes a frequent problem in endemic areas. Basing our study on the hypothesis that descendants of a residual population should be more similar to the pre-treatment population than to any other, we compared the indications of two quantitative morphological approaches. This study seeks to find the origin of 247 T. infestans from three populations found in two chicken coops and a goat corral after treatment with insecticides. The results obtained by quantitative morphology suggest that the T. infestans found between three-34 months after the application of insecticides formed mixed populations with insects derived from residual foci and neighbouring habitats. Our analyses also showed the presence of a phenotype which does not resemble neither the pre-treatment phenotype nor the one from neighbouring populations, suggesting the presence of a particular post-treatment phenotype. The heads size showed some variations in males from different populations and remained unchanged in females, which reinforces the hypothesis of an intraspecific competition for food with priority for females. This article presents, for the first time, the combined analysis of geometric morphometry of heads and antennal phenotypes to identify the composition of reinfesting populations.

  9. A mass collection of Triatoma ryckmani (Hemiptera: Reduviidae) from Stenocereus Eichlamii (Cactaceae) in the semiarid region of Guatemala.

    Science.gov (United States)

    Marroquín, Ricardo; Bor, Silvia; Monroy, M Carlota

    2004-12-01

    A population of 216 specimens of Stenocereus eichlamii (Cactaceae, Subfamily Cereoideae) was surveyed for Triatoma ryckmani (vector of Chagas disease) in a one hectare plot of semiarid habitat in Guatemala. Out of 44 plants that had dead and dry sections, 24 plants had a total of 103 specimens of T. ryckmani. In comparison with other areas of Guatemala, T ryckmani is well established in the semiarid ecosystem (Infestation index 54.5, density = 2.3 and crowding index 4.3). The insects were mainly found (52.4%) in the dead portions of S. eichlamii 2.0 to 3.2 m above the ground (X2 = 26.0, P < 0.00001), followed by dry cactus sections between 3.2 and 5.0 m (35.9%). They were less frequent 0.2 to 2.0 m above the ground. A considerable proportion (75.7%) had no aparent blood in their digestive systems. To determine the presence of flagellates, 43 of the bugs were dissected, but none were found. This is the first report on T. ryckmani population dynamics in this habitat.

  10. New structures for goat corrals to control peridomestic populations of Triatoma infestans (Hemiptera: Reduviidae in the Gran Chaco of Argentina

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    David Eladio Gorla

    2013-05-01

    Full Text Available Goat production is an important economic activity for rural communities in the Gran Chaco of Argentina. Goat corrals are important for the survival of peridomestic populations of Triatoma infestans. This study evaluated the impact of modifying the traditional structure of goat corrals on T. infestans populations and goat productivity in the region of Los Llanos (La Rioja. Thirty-nine experimental corrals were constructed and 57 traditional corrals were used as controls. We evaluated the infestations of the control and experimental corrals for five years following construction of the structures. The results showed that the new structures did not prevent the colonization, although it enhanced the detection of infestation at low densities of T. infestans. No significant difference was found in T. infestans population abundance between control and experimental corrals, probably because of the different detectability in the two types of structures, especially among the small nymphs. Although goat productivity average was higher in experimental than in control corrals, no significant difference was found because of high variability. The new structures can be used as a complement to promote the development of rural communities. Acceptability and adoption of the new corrals by the owners was high, as the enclosures offered better protection for the goats, increased growth of kids and facilitated herd handling.

  11. Where do these bugs come from? Phenotypic structure of Triatoma infestans populations after control interventions in the Argentine Chaco

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    María Sol Gaspe

    2015-05-01

    Full Text Available House re-invasion by native triatomines after insecticide-based control campaigns represents a major threat for Chagas disease vector control. We conducted a longitudinal intervention study in a rural section (Area III, 407 houses of Pampa del Indio, northeastern Argentina, and used wing geometric morphometry to compare pre-spray and post-spray (re-infestant bugs Triatoma infestans populations. The community-wide spraying with pyrethroids reduced the prevalence of house infestation by T. infestans from 31.9% to < 1% during a four-year follow-up, unlike our previous studies in the neighbouring Area I. Two groups of bug collection sites differing in wing shape variables before interventions (including 221 adults from 11 domiciles were used as a reference for assigning 44 post-spray adults. Wing shape variables from post-spray, high-density bug colonies and pre-spray groups were significantly different, suggesting that re-infestant insects had an external origin. Insects from one house differed strongly in wing shape variables from all other specimens. A further comparison between insects from both areas supported the existence of independent re-infestation processes within the same district. These results point to local heterogeneities in house re-infestation dynamics and emphasise the need to expand the geographic coverage of vector surveillance and control operations to the affected region.

  12. Evaluation of risk factors for rural infestation by Triatoma pallidipennis (Hemiptera: Triatominae), a Mexican vector of Chagas disease.

    Science.gov (United States)

    Enger, Kyle S; Ordoñez, Rosalinda; Wilson, Mark L; Ramsey, Janine M

    2004-07-01

    Control of Chagas disease requires control of its triatomine vectors, which requires an understanding of the determinants of infestation. Twenty-seven household environmental characteristics in the town of Chalcatzingo, Morelos, were analyzed for association with infestation by Triatoma pallidipennis, the predominant local vector. Data were obtained through timed household searches for triatomines and surveys that characterized intradomicile and peridomicile environments. Of the households surveyed, 28.4% were infested by T. pallidipennis. Cross-sectional multivariate logistic regression analyses were performed that regressed infestation on environmental variables. Of the 530 households in the town, 84% had sufficient data to be included. Adobe walls, agricultural products, junk piles, lack of bednets, and number of rabbits were significantly associated with intradomiciliary infestation. Junk piles and numbers of dogs, cats, and rabbits were significantly associated with peridomiciliary infestation. Junk piles, agricultural products, and numbers of cats, rabbits, and birds were significantly associated with overall infestation. Unexpectedly, presence of stone piles was not associated with infestation. The results of this study provide information for designing Chagas disease control programs in rural Mexican areas infested by T. pallidipennis.

  13. Toxicological and biochemical analysis of the susceptibility of sylvatic Triatoma infestans from the Andean Valley of Bolivia to organophosphate insecticide

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    Pablo Luis Santo-Orihuela

    2013-09-01

    Full Text Available To increase our knowledge of the natural susceptibility of Triatoma infestans to an organophosphate insecticide, we performed toxicological and biochemical studies on three sylvatic populations from Bolivia and two populations from domestic dwellings from Bolivia and Argentina. Fifty-per-cent lethal doses (LD50 were determined based on the topical application of fenitrothion on first instar nymphs and mortality was assessed at 24 h. Both type of populations exhibited LD50ratios significantly higher than 1 with a range of the values (1.42-2.47; the maximum value were found in a sylvatic (-S population, Veinte de Octubre-S. Samples were biochemically analysed using a glutathione S-transferase activity assay. The highest significant activity was obtained for Veinte de Octubre-S and the lowest activity was obtained for the reference population (102.69 and 54.23 pmol per minute per mg of protein respectively. Two out of the three sylvatic populations (Veinte de Octubre-S and Kirus Mayu-S exhibited significantly higher glutathione S-transferase activity than that of the reference population. Based on this analysis of the natural susceptibility of this organism to organophosphate insecticides, continental and focal surveys of organophosphate susceptibility should be conducted to evaluate the evolution and distribution of this phenomenon.

  14. X-ray structure of Triatoma virus empty capsid: insights into the mechanism of uncoating and RNA release in dicistroviruses.

    Science.gov (United States)

    Sánchez-Eugenia, Rubén; Durana, Aritz; López-Marijuan, Ibai; Marti, Gerardo A; Guérin, Diego M A

    2016-10-01

    In viruses, uncoating and RNA release are two key steps of successfully infecting a target cell. During these steps, the capsid must undergo the necessary conformational changes to allow RNA egress. Despite their importance, these processes are poorly understood in the family Dicistroviridae. Here, we used X-ray crystallography to solve the atomic structure of a Triatoma virus(TrV) empty particle (Protein Data Bank ID 5L7O), which is the resulting capsid after RNA release. It is observed that the overall shape of the capsid and of the three individual proteins is maintained in comparison with the mature virion. Furthermore, no channels indicative of RNA release are formed in the TrV empty particle. However, the most prominent change in the empty particle when compared with the mature virion is the loss of order in the N-terminal domain of the VP2 protein. In mature virions, the VP2 N-terminal domain of one pentamer is swapped with its twofold related copy in an adjacent pentamer, thereby stabilizing the binding between the pentamers. The loss of these interactions allows us to propose that RNA release may take place through transient flipping-out of pentameric subunits. The lower number of stabilizing interactions between the pentamers and the lack of formation of new holes support this model. This model differs from the currently accepted model for rhinoviruses and enteroviruses, in which genome externalization occurs by extrusion of the RNA through capsid channels.

  15. Toxicity of pyrethroids and repellency of diethyltoluamide in two deltamethrin-resistant colonies of Triatoma infestans Klug, 1834 (Hemiptera: Reduviidae

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    Valeria Sfara

    2006-02-01

    Full Text Available The aim of the currrent investigation was to evaluate (a the toxicity of three pyrethroids (deltamethrin, lambda-cyhalothrin, and tetramethrin; (b the effect of these insecticides on the locomotor activity; and (c the repellent effect of N,N-diethyl-m-toluamide (DEET on two deltamethrin-resistant strains of Triatoma infestans from Argentina (El Chorro and La Toma, and one susceptible strain. The resistance ratios (RRs obtained for the La Toma strain were: > 10,769, 50.7, and > 5.2 for deltamethrin, lambda-cyhalothrin, and tetramethrin respectively. The RRs for the El Chorro strain were: > 10,769, 85.8, and > 5.2 for deltamethrin, lambda-cyhalothrin, and tetramethrin respectively. The hyperactivity usually caused by the three pyrethroids was in both the deltamethrin-resistant strains compared to the susceptible reference strain. No differences were observed in the repellent effect of DEET between the three groups. These results indicate that the deltamethrin-resistant insects have a cross resistance to lambda-cyhalothrin and tetramethrin, and are also resistant to the first symptom of pyrethroid poisoning (hyperactivity. However, the sensorial process related to DEET repellency does not appear to be altered.

  16. Potential for entomopathogenic fungi to control Triatoma dimidiata (Hemiptera: Reduviidae, a vector of Chagas disease in Mexico

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    María Guadalupe Vázquez-Martínez

    2014-12-01

    Full Text Available Introduction The use of entomopathogenic fungi to control disease vectors has become relevant because traditional chemical control methods have caused damage to the environment and led to the development of resistance among vectors. Thus, this study assessed the pathogenicity of entomopathogenic fungi in Triatoma dimidiata. Methods Preparations of 108 conidia/ml of Gliocladium virens, Talaromyces flavus, Beauveria bassiana and Metarhizium anisopliae were applied topically on T. dimidiata nymphs and adults. Controls were treated with the 0.0001% Tween-80 vehicle. Mortality was evaluated and recorded daily for 30 days. The concentration required to kill 50% of T. dimidiata (LC50 was then calculated for the most pathogenic isolate. Results Pathogenicity in adults was similar among B. bassiana, G. virens and T. flavus (p>0.05 and differed from that in triatomine nymphs (p=0.009. The most entomopathogenic strains in adult triatomines were B. bassiana and G. virens, which both caused 100% mortality. In nymphs, the most entomopathogenic strain was B. bassiana, followed by G. virens. The native strain with the highest pathogenicity was G. virens, for which the LC50 for T. dimidiata nymphs was 1.98 x108 conidia/ml at 13 days after inoculation. Conclusions Beauveria bassiana and G. virens showed entomopathogenic potential in T. dimidiata nymphs and adults. However, the native G. virens strain presents a higher probability of success in the field, and G. virens should thus be considered a potential candidate for the biological control of triatomine Chagas disease vectors.

  17. CHICKEN COOPS, Triatoma dimidiata INFESTATION AND ITS INFECTION WITH Trypanosoma cruzi IN A RURAL VILLAGE OF YUCATAN, MEXICO.

    Science.gov (United States)

    Koyoc-Cardeña, Edgar; Medina-Barreiro, Anuar; Escobedo-Ortegón, Francisco Javier; Rodríguez-Buenfil, Jorge Carlos; Barrera-Pérez, Mario; Reyes-Novelo, Enrique; Chablé-Santos, Juan; Selem-Salas, Celia; Vazquez-Prokopec, Gonzalo; Manrique-Saide, Pablo

    2015-01-01

    This study longitudinally investigated the association between Triatoma dimidiata infestation, triatomine infection with Trypanosoma cruzi and household/backyard environmental characteristics in 101 homesteads in Molas and Yucatan, Mexico, between November 2009 (rainy season) and May 2010 (dry season). Logistic regression models tested the associations between insect infestation/infection and potential household-level risk factors. A total of 200 T. dimidiata were collected from 35.6% of the homesteads, mostly (73%) from the peridomicile. Of all the insects collected, 48% were infected with T. cruzi. Infected insects were collected in 31.6% of the homesteads (54.1% and 45.9% intra- and peridomiciliary, respectively). Approximately 30% of all triatomines collected were found in chicken coops. The presence of a chicken coop in the backyard of a homestead was significantly associated with both the odds of finding T. dimidiata (OR = 4.10, CI 95% = 1.61-10.43, p = 0.003) and the presence of triatomines infected with T. cruzi (OR = 3.37, CI 95% = 1.36-8.33, p = 0.006). The results of this study emphasize the relevance of chicken coops as a putative source of T. dimidiata populations and a potential risk for T. cruzi transmission.

  18. CHICKEN COOPS, Triatoma dimidiata INFESTATION AND ITS INFECTION WITH Trypanosoma cruzi IN A RURAL VILLAGE OF YUCATAN, MEXICO

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    Edgar KOYOC-CARDEÑA

    2015-06-01

    Full Text Available This study longitudinally investigated the association between Triatoma dimidiata infestation, triatomine infection with Trypanosoma cruzi and household/backyard environmental characteristics in 101 homesteads in Molas and Yucatan, Mexico, between November 2009 (rainy season and May 2010 (dry season. Logistic regression models tested the associations between insect infestation/infection and potential household-level risk factors. A total of 200 T. dimidiata were collected from 35.6% of the homesteads, mostly (73% from the peridomicile. Of all the insects collected, 48% were infected with T. cruzi. Infected insects were collected in 31.6% of the homesteads (54.1% and 45.9% intra- and peridomiciliary, respectively. Approximately 30% of all triatomines collected were found in chicken coops. The presence of a chicken coop in the backyard of a homestead was significantly associated with both the odds of finding T. dimidiata (OR = 4.10, CI 95% = 1.61-10.43, p = 0.003 and the presence of triatomines infected with T. cruzi (OR = 3.37, CI 95% = 1.36-8.33, p = 0.006. The results of this study emphasize the relevance of chicken coops as a putative source of T. dimidiata populations and a potential risk for T. cruzi transmission.

  19. Direito e Risco: do Consumo ao Ambiente

    OpenAIRE

    Marques, Carlos Alexandre Michaello

    2014-01-01

    A presente Dissertação tem como tema de investigação “Direito e Risco: do Consumo ao Ambiente”. Para tanto, essa temática foi delimitada através da investigação sobre a capacidade do Direito a partir de uma leitura Sociológica e Antropológica de compreender e promover aprendizagem nas questões que envolvem o Risco como elemento decorrente do Consumo e que externa suas consequências ao Ambiente. Neste sentido, se direcionou a responder ao problema de saber: em que medida é possível e necessári...

  20. Conducta alimentaria de poblaciones de triatoma dimidiata (hemiptera: reduviidae: triatominae) en una zona endémica y sus implicaciones epidemiológicas

    OpenAIRE

    Farfán-García, Ana E.; Angulo-Silva, Víctor M.

    2012-01-01

    Objetivo Determinar la conducta alimentaria de Triatoma dimidiata en hábitats domiciliarios y extradomiciliarios en una zona endémica de Santander, Colombia. Materiales y métodos Mediante la técnica de ELISA, se procesaron 367 contenidos intestinales de insectos capturados en zonas rurales de los municipios de Capitanejo y Macaravita. Estos fueron procesados por medio de la técnica de ELISA, con 12 antisueros policlonales anti-especie animal específicos. Los hospederos de T. dimidiata fueron ...

  1. Triatoma delpontei Romaña & Abalos, 1947 (Hemiptera, Triatominae) en el estado brasileño de "Rio Grande do Sul"

    OpenAIRE

    Agrelo,Roberto Salvatella; Basmadjian,Yester; Rosa,Raquel; Puime,Andres

    1993-01-01

    Triatoma delpontei Romaña y Abalos, 1947 es una especie de triatomineo silvestre, de marcada ornitofilia, que se asocia preferentemente al psitácido Myiopsitta monachus (Boddaert, 1783) colonizando sus nidos. Especie que se identifica con zonas de forestas subtropicales secas o xerofíticas propias de las provincias biogeográficas continentales chaqueñas, es notificada para Brasil en la microregión de la campaña de Río Grande do Sul (barra de Quaraí, Municipio de Uruguaiana).

  2. Triatoma mexicana Herrich-Schaeffer (Hemiptera: Reduviidae: Triatominae) Descripción de la Genitalia Externa del Macho y Morfología Externa de la Hembra.

    OpenAIRE

    2013-01-01

    Triatoma mexicana fue descrita originalmente en 1848 por Herrich-Schaeffer nombrándola Conorhinus mexicanus Herrich-Schaeffer. Actualmente se registra su hallazgo en los estados de Guanajuato, Hidalgo Querétaro y San Luis Potosí. Los estudios sobre su distribución espacial, índices entomológicos, presencia en el intradomicilio y peridomicilio, biología y riesgo de transmisión de Trypanosoma cruzi Chagas demuestran que es una especie impo...

  3. A common Caatinga cactus, Pilosocereus gounellei, is an important ecotope of wild Triatoma brasiliensis populations in the Jaguaribe valley of northeastern Brazil.

    Science.gov (United States)

    Valença-Barbosa, Carolina; Lima, Marli M; Sarquis, Otília; Bezerra, Claudia M; Abad-Franch, Fernando

    2014-06-01

    Triatoma brasiliensis is the most important vector of Chagas disease in the Caatinga eco-region of northeastern Brazil. Wild T. brasiliensis populations have been reported only from rocky outcrops. However, this species frequently infests/re-infests houses in rock-free sedimentary lowlands. We therefore hypothesized that it should also occupy other natural ecotopes. We show that a common Caatinga cactus, Pilosocereus gounellei, locally known as xiquexique, often harbors T. brasiliensis breeding colonies apparently associated with rodents (n = 44 cacti, infestation rate = 47.7%, 157 bugs captured). Our findings suggest that infested cacti might be involved in house re-infestation by T. brasiliensis in the Caatinga region.

  4. Characterization of Guinea Pig Antibody Responses to Salivary Proteins of Triatoma infestans for the Development of a Triatomine Exposure Marker

    Science.gov (United States)

    Dorňáková, Veronika; Salazar-Sanchez, Renzo; Borrini-Mayori, Katty; Carrion-Navarro, Oscar; Levy, Michael Z.; Schaub, Günter A.; Schwarz, Alexandra

    2014-01-01

    Background Salivary proteins of Triatoma infestans elicit humoral immune responses in their vertebrate hosts. These immune responses indicate exposure to triatomines and thus can be a useful epidemiological tool to estimate triatomine infestation. In the present study, we analyzed antibody responses of guinea pigs to salivary antigens of different developmental stages of four T. infestans strains originating from domestic and/or peridomestic habitats in Argentina, Bolivia, Chile and Peru. We aimed to identify developmental stage- and strain-specific salivary antigens as potential markers of T. infestans exposure. Methodology and Principal Findings In SDS-PAGE analysis of salivary proteins of T. infestans the banding pattern differed between developmental stages and strains of triatomines. Phenograms constructed from the salivary profiles separated nymphal instars, especially the 5th instar, from adults. To analyze the influence of stage- and strain-specific differences in T. infestans saliva on the antibody response of guinea pigs, twenty-one guinea pigs were exposed to 5th instar nymphs and/or adults of different T. infestans strains. Western blot analyses using sera of exposed guinea pigs revealed stage- and strain-specific variations in the humoral response of animals. In total, 27 and 17 different salivary proteins reacted with guinea pig sera using IgG and IgM antibodies, respectively. Despite all variations of recognized salivary antigens, an antigen of 35 kDa reacted with sera of almost all challenged guinea pigs. Conclusion Salivary antigens are increasingly considered as an epidemiological tool to measure exposure to hematophagous arthropods, but developmental stage- and strain-specific variations in the saliva composition and the respective differences of immunogenicity are often neglected. Thus, the development of a triatomine exposure marker for surveillance studies after triatomine control campaigns requires detailed investigations. Our study resulted

  5. Environmental management for the control of Triatoma dimidiata (Latreille, 1811, (Hemiptera: Reduviidae in Costa Rica: a pilot project

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    Rodrigo Zeledón

    2006-06-01

    Full Text Available An ecological control method, using environmental management operations, based on biological and behavioral characteristics of Triatoma dimidiata (Latreille, 1811, was implemented as a pilot project in an area of Costa Rica where the bug is prevalent. The sample was represented by 20 houses with peridomestic colonies (two also had indoor infestation, divided in two equivalent groups of 10 each. In one group we intervened the houses, i.e. all objects or materials that were serving as artificial ecotopes for the bugs were removed, and the second group was used as control houses. After a year of periodic follow up, it became evident that in those houses with a modified environment the number of insects had decreased notoriously even after the first visits and this was more evident after a period of 12.5 to 13.5 months in which no insects were detected in eight of the houses. It also became clear that in this group of houses, recolonization by wild bugs from the surrounding areas, became more difficult, probably due to the absence of protection from bug predators. In the control houses, with the exception of three in which the inhabitants decided to intervene on their own, and another house with a peculiar situation, the insect populations remained the same or even showed a tendency to increase, as confirmed at the end of the experiment. We believe that the method is feasible, low costing and non contaminating. It could be used successfully in other places where T. dimidiata is common and also in countries where other species colonize peridomestic areas of homes. Environmental management of this kind should seek the participation of the members of the communities, in order to make it a more permanent control measure.

  6. Domestic, peridomestic and wild hosts in the transmission of Trypanosoma cruzi in the Caatinga area colonised by Triatoma brasiliensis

    Science.gov (United States)

    Bezerra, Claudia Mendonça; Cavalcanti, Luciano Pamplona de Góes; de Souza, Rita de Cássia Moreira; Barbosa, Silvia Ermelinda; Xavier, Samanta Cristina das Chagas; Jansen, Ana Maria; Ramalho, Relrison Dias; Diotaiut, Liléia

    2014-01-01

    The role played by different mammal species in the maintenance of Trypanosoma cruzi is not constant and varies in time and place. This study aimed to characterise the importance of domestic, wild and peridomestic hosts in the transmission of T. cruzi in Tauá, state of Ceará, Caatinga area, Brazil, with an emphasis on those environments colonised by Triatoma brasiliensis. Direct parasitological examinations were performed on insects and mammals, serologic tests were performed on household and outdoor mammals and multiplex polymerase chain reaction was used on wild mammals. Cytochrome b was used as a food source for wild insects. The serum prevalence in dogs was 38% (20/53), while in pigs it was 6% (2/34). The percentages of the most abundantly infected wild animals were as follows: Thrichomys laurentius 74% (83/112) and Kerodon rupestris 10% (11/112). Of the 749 triatomines collected in the household research, 49.3% (369/749) were positive for T. brasiliensis, while 6.8% were infected with T. cruzi (25/369). In captured animals, T. brasiliensis shares a natural environment with T. laurentius, K. rupestris, Didelphis albiventris, Monodelphis domestica, Galea spixii, Wiedomys pyrrhorhinos, Conepatus semistriatus and Mus musculus. In animals identified via their food source, T. brasiliensis shares a natural environment with G. spixii, K. rupestris, Capra hircus, Gallus gallus, Tropidurus oreadicus and Tupinambis merianae. The high prevalence of T. cruzi in household and peridomiciliar animals reinforces the narrow relationship between the enzootic cycle and humans in environments with T. brasiliensis and characterises it as ubiquitous. PMID:25410992

  7. Domestic, peridomestic and wild hosts in the transmission of Trypanosoma cruzi in the Caatinga area colonised by Triatoma brasiliensis.

    Science.gov (United States)

    Bezerra, Claudia Mendonça; Cavalcanti, Luciano Pamplona de Góes; Souza, Rita de Cássia Moreira de; Barbosa, Silvia Ermelinda; Xavier, Samanta Cristina das Chagas; Jansen, Ana Maria; Ramalho, Relrison Dias; Diotaiut, Liléia

    2014-08-22

    The role played by different mammal species in the maintenance of Trypanosoma cruzi is not constant and varies in time and place. This study aimed to characterise the importance of domestic, wild and peridomestic hosts in the transmission of T. cruzi in Tauá, state of Ceará, Caatinga area, Brazil, with an emphasis on those environments colonised by Triatoma brasiliensis. Direct parasitological examinations were performed on insects and mammals, serologic tests were performed on household and outdoor mammals and multiplex polymerase chain reaction was used on wild mammals. Cytochrome b was used as a food source for wild insects. The serum prevalence in dogs was 38% (20/53), while in pigs it was 6% (2/34). The percentages of the most abundantly infected wild animals were as follows: Thrichomys laurentius 74% (83/112) and Kerodon rupestris 10% (11/112). Of the 749 triatomines collected in the household research, 49.3% (369/749) were positive for T. brasiliensis, while 6.8% were infected with T. cruzi (25/369). In captured animals, T. brasiliensis shares a natural environment with T. laurentius, K. rupestris, Didelphis albiventris, Monodelphis domestica, Galea spixii, Wiedomys pyrrhorhinos, Conepatus semistriatus and Mus musculus. In animals identified via their food source, T. brasiliensis shares a natural environment with G. spixii, K. rupestris, Capra hircus, Gallus gallus, Tropidurus oreadicus and Tupinambis merianae. The high prevalence of T. cruzi in household and peridomiciliar animals reinforces the narrow relationship between the enzootic cycle and humans in environments with T. brasiliensis and characterises it as ubiquitous.

  8. Molecular evidence of demographic expansion of the chagas disease vector Triatoma dimidiata (Hemiptera, Reduviidae, Triatominae in Colombia.

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    Andrés Gómez-Palacio

    2014-03-01

    Full Text Available BACKGROUND: Triatoma dimidiata is one of the most significant vectors of Chagas disease in Central America and Colombia, and, as in most species, its pattern of genetic variation within and among populations is strongly affected by its phylogeographic history. A putative origin from Central America has been proposed for Colombian populations, and high genetic differentiation among three biographically different population groups has recently been evidenced. Analyses based on putatively neutral markers provide data from which past events, such as population expansions and colonization, can be inferred. We analyzed the genealogies of the nicotinamide adenine dinucleotide dehydrogenase 4 (ND4 and the cytochrome oxidase subunit 1-mitochondrial genes, as well as partial nuclear ITS-2 DNA sequences obtained across most of the eco-geographical range in Colombia, to assess the population structure and demographic factors that may explain the geographical distribution of T. dimidiata in this country. RESULTS: The population structure results support a significant association between genetic divergence and the eco-geographical location of population groups, suggesting that clear signals of demographic expansion can explain the geographical distribution of haplotypes of population groups. Additionally, empirical date estimation of the event suggests that the population's expansion can be placed after the emergence of the Panama Isthmus, and that it was possibly followed by a population fragmentation process, perhaps resulting from local adaptation accomplished by orographic factors such as geographical isolation. CONCLUSION: Inferences about the historical population processes in Colombian T. dimidiata populations are generally in accordance with population expansions that may have been accomplished by two important biotic and orographic events such as the Great American Interchange and the uplift of the eastern range of the Andes mountains in central

  9. A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota.

    Science.gov (United States)

    Buarque, Diego S; Gomes, Cícera M; Araújo, Ricardo N; Pereira, Marcos H; Ferreira, Roberta C; Guarneri, Alessandra A; Tanaka, Aparecida S

    2016-04-01

    The Reduviid Triatoma infestans is a vector for the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. The parasite must address the defense molecules and microbiota that colonize the anterior midgut of T. infestans. To obtain insight into T. cruzi - microbiota interactions in triatomine insects, we characterized a new antimicrobial product from the anterior midgut of T. infestans (TiAP) that may be involved in these relationships. The TiAP DNA fragment was cloned and expressed in a bacterial system, and the effect of the protein on bacteria and T. cruzi was evaluated by RNAi, qPCR and antimicrobial experiments. The number of T. cruzi in T. infestans anterior midguts was significantly lower in TiAP knockdown insects than in unsilenced groups. We also verified that the amount of bacteria in silenced T. infestans is approximately 600-fold higher than in unsilenced insects by qPCR. The 327-bp cDNA fragment that encodes mature TiAP was cloned into the pET-14b vector and expressed fused to a His-tag in Escherichia coli C43. The recombinant protein (rTiAP) was purified using an Ni-NTA column, followed by a HiTrap SP column. According to a trypanocidal assay, rTiAP did not interfere with the viability of T. cruzi trypomastigotes. Moreover, in antimicrobial experiments using E. coli and Micrococcus luteus, the protein was only bacteriostatic for Gram-negative bacteria. The data indicate that infection by T. cruzi increases the expression of TiAP to modulate the microbiota. The inhibition of microbiota growth by TiAP is important for parasite establishment in the T. infestans anterior midgut.

  10. Domestic, peridomestic and wild hosts in the transmission of Trypanosoma cruzi in the Caatinga area colonised by Triatoma brasiliensis

    Directory of Open Access Journals (Sweden)

    Claudia Mendonça Bezerra

    2014-11-01

    Full Text Available The role played by different mammal species in the maintenance of Trypanosoma cruzi is not constant and varies in time and place. This study aimed to characterise the importance of domestic, wild and peridomestic hosts in the transmission of T. cruzi in Tauá, state of Ceará, Caatinga area, Brazil, with an emphasis on those environments colonised by Triatoma brasiliensis. Direct parasitological examinations were performed on insects and mammals, serologic tests were performed on household and outdoor mammals and multiplex polymerase chain reaction was used on wild mammals. Cytochrome b was used as a food source for wild insects. The serum prevalence in dogs was 38% (20/53, while in pigs it was 6% (2/34. The percentages of the most abundantly infected wild animals were as follows: Thrichomys laurentius 74% (83/112 and Kerodon rupestris 10% (11/112. Of the 749 triatomines collected in the household research, 49.3% (369/749 were positive for T. brasiliensis, while 6.8% were infected with T. cruzi (25/369. In captured animals, T. brasiliensis shares a natural environment with T. laurentius, K. rupestris, Didelphis albiventris, Monodelphis domestica, Galea spixii, Wiedomys pyrrhorhinos, Conepatus semistriatus and Mus musculus. In animals identified via their food source, T. brasiliensis shares a natural environment with G. spixii, K. rupestris, Capra hircus, Gallus gallus, Tropidurus oreadicus and Tupinambis merianae. The high prevalence of T. cruzi in household and peridomiciliar animals reinforces the narrow relationship between the enzootic cycle and humans in environments with T. brasiliensis and characterises it as ubiquitous.

  11. Changes associated with laboratory rearing in antennal sensilla patterns of Triatoma infestans, Rhodnius prolixus, and Rhodnius pallescens (Hemiptera, Reduviidae, Triatominae

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    Catalá SS

    2004-01-01

    Full Text Available We examined changes in the array of antennal sensilla of three species of Triatominae (Triatoma infestans, Rhodnius prolixus, and R. pallescens following their establishment for different periods in laboratory culture. In each case, the laboratory colonies were compared with conspecific samples taken directly from the field, by quantitative analysis of the sensilla arrays on the three distal segments of the antenna in terms of the densities of three types of chemoreceptors (basiconics and thick and thin walled trichoids and one type of mechanoreceptor (bristles. Sensilla densities were compared by ANOVA or non-parametric tests, and by multivariate discriminant analysis. Strains of the same species reared in different laboratories showed significant differences in their sensilla arrays, especially when compared to field-collected material from the same geographic origin. A Bolivian strain of T. infestans reared in the laboratory for 15 years and fed at monthly intervals, showed greatest differences from its conspecific wild forms, especially in terms of reductions in the number of chemoreceptors. By contrast, an Argentine strain of T. infestans reared for 25 years in the laboratory and fed weekly, showed a relative increase in the density of mechanoreceptors. A Colombian strain of R. prolixus reared for 20 years and fed weekly or fortnightly, showed only modest differences in the sensilla array when compared to its wild populations from the same area. However, a Colombian strain of R. pallescens reared for 12 years and fed fortnightly, did show highly significant reductions in one form of chemoreceptor compared to its conspecific wild populations. For all populations, multivariate analysis clearly discriminated between laboratory and field collected specimens, suggesting that artificial rearing can lead to modifications in the sensory array. This not only supports the idea of morphological plasticity in these species, but also suggests caution in

  12. Host-Seeking Behavior and Dispersal of Triatoma infestans, a Vector of Chagas Disease, under Semi-field Conditions

    Science.gov (United States)

    Castillo-Neyra, Ricardo; Barbu, Corentin M.; Salazar, Renzo; Borrini, Katty; Naquira, Cesar; Levy, Michael Z.

    2015-01-01

    Chagas disease affects millions of people in Latin America. The control of this vector-borne disease focuses on halting transmission by reducing or eliminating insect vector populations. Most transmission of Trypanosoma cruzi, the causative agent of Chagas disease, involves insects living within or very close to households and feeding mostly on domestic animals. As animal hosts can be intermittently present it is important to understand how host availability can modify transmission risk to humans and to characterize the host-seeking dispersal of triatomine vectors on a very fine scale. We used a semi-field system with motion-detection cameras to characterize the dispersal of Triatoma infestans, and compare the behavior of vector populations in the constant presence of hosts (guinea pigs), and after the removal of the hosts. The emigration rate – net insect population decline in original refuge – following host removal was on average 19.7% of insects per 10 days compared to 10.2% in constant host populations (p = 0.029). However, dispersal of T. infestans occurred in both directions, towards and away from the initial location of the hosts. The majority of insects that moved towards the original location of guinea pigs remained there for 4 weeks. Oviposition and mortality were observed and analyzed in the context of insect dispersal, but only mortality was higher in the group where animal hosts were removed (p-value vector control. Removing domestic animals in infested areas increases vector dispersal from the first day of host removal. The implications of these patterns of vector dispersal in a field setting are not yet known but could result in movement towards human rooms. PMID:25569228

  13. Characterization of guinea pig antibody responses to salivary proteins of Triatoma infestans for the development of a triatomine exposure marker.

    Directory of Open Access Journals (Sweden)

    Veronika Dorňáková

    2014-04-01

    Full Text Available BACKGROUND: Salivary proteins of Triatoma infestans elicit humoral immune responses in their vertebrate hosts. These immune responses indicate exposure to triatomines and thus can be a useful epidemiological tool to estimate triatomine infestation. In the present study, we analyzed antibody responses of guinea pigs to salivary antigens of different developmental stages of four T. infestans strains originating from domestic and/or peridomestic habitats in Argentina, Bolivia, Chile and Peru. We aimed to identify developmental stage- and strain-specific salivary antigens as potential markers of T. infestans exposure. METHODOLOGY AND PRINCIPAL FINDINGS: In SDS-PAGE analysis of salivary proteins of T. infestans the banding pattern differed between developmental stages and strains of triatomines. Phenograms constructed from the salivary profiles separated nymphal instars, especially the 5th instar, from adults. To analyze the influence of stage- and strain-specific differences in T. infestans saliva on the antibody response of guinea pigs, twenty-one guinea pigs were exposed to 5th instar nymphs and/or adults of different T. infestans strains. Western blot analyses using sera of exposed guinea pigs revealed stage- and strain-specific variations in the humoral response of animals. In total, 27 and 17 different salivary proteins reacted with guinea pig sera using IgG and IgM antibodies, respectively. Despite all variations of recognized salivary antigens, an antigen of 35 kDa reacted with sera of almost all challenged guinea pigs. CONCLUSION: Salivary antigens are increasingly considered as an epidemiological tool to measure exposure to hematophagous arthropods, but developmental stage- and strain-specific variations in the saliva composition and the respective differences of immunogenicity are often neglected. Thus, the development of a triatomine exposure marker for surveillance studies after triatomine control campaigns requires detailed

  14. Pre-exposure to faeces or saliva of Triatoma dimidiata decreases parasitemia in mice challenged with Trypanosoma cruzi: a description of the inflammatory reaction at the inoculation site

    Science.gov (United States)

    Monteon, Victor; Quen-Rámirez, Enrique; Macedo-Reyes, Veronica; Lopez, Ruth; Acosta-Viana, Karla; Pennigton, Pamela; Ramos-Ligonio, Angel

    2016-10-01

    Under natural conditions, Trypanosoma cruzi infection is transmitted to mammals when faeces contaminated with metacyclic trypomastigotes gain access through skin lesions, mucosa or bite wounds. Natural infection of bugs with T. cruzi can vary greatly from less than 1% up to 70%, depending on triatomine species: in the case of Triatoma dimidiata, the percentage of infection is around 30%. In this work uses biological fluids (saliva and faeces) from Triatoma dimidiata to inoculate experimental animals once or multiple times, before inoculation with faeces contaminated with metacyclic trypomastigotes discrete type unit Ia (TcI). The site of infection was analyzed for histological changes based on hematoxile-eosine technique and toluide blue stain for mast cells. Inoculation with saliva led to the recruitment of eosinophils and mononuclear cells at the inoculation site, whereas inoculation with faeces led to the recruitment of neutrophils. Mice inoculated multiple times exhibited a strong inflammatory reaction from the first hour. Mono- or multi-exposure to T. dimidiata fluids before inoculation with metacyclic trypomastigotes helped to control the level of parasitemia. Previous contact with saliva or faeces of T. dimidiata reduces parasitemia in T. cruzi I -infected mice.

  15. Alergia ao látex

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    Allarcon Jorge Barrios

    2003-01-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O látex, (cis 1,4 polyisoprene está muito presente no dia-a-dia do anestesiologista. Os primeiros relatos de alergia ao látex datam de 1933. A sensibilidade ao látex na população de anestesiologistas é de 12,5% a 15,8%. A incidência de sensibilidade ao látex aumenta muito em grupos específicos, podendo produzir reações graves e até mesmo fatais. O objetivo desse estudo foi realizar uma revisão bibliográfica sobre o assunto, abordando este tema de interesse para o anestesiologista, considerando diagnóstico, prevenção e tratamento da alergia ao látex. CONTEÚDO: Os estudos sobre a prevalência das reações alérgicas ao látex variam muito conforme a população estudada e os métodos utilizados para estabelecer a sensibilidade ao antígeno. Mesmo com estas variações, existem alguns grupos em que o risco é elevado, particularmente em pacientes com distúrbio na formação do tubo neural. As manifestações clínicas variam desde dermatite de contato até anafilaxia e óbito. O diagnóstico precoce com pesquisa de IgE pode prevenir as reações de hipersensibilidade. CONCLUSÕES: É de extrema importância o diagnóstico precoce da alergia ao látex. A abordagem desses pacientes em ambientes livres de látex evitam a sensibilização e evolução muitas vezes para quadros dramáticos.

  16. Triatominae survey (Hemiptera: Reduviidae: Triatominae) in the south-central region of the state of Bahia, Brazil between 2008 and 2013.

    Science.gov (United States)

    Mendonça, Vagner José; de Oliveira, Jader; Rimoldi, Aline; Filho, Júlio C R Ferreira; de Araújo, Renato Freitas; da Rosa, João Aristeu

    2015-05-01

    Triatomine surveillance in rural areas, artificial ecotypes, and natural ecotopes of the cities of Caturama, Ibipitanga, Macaúbas, and Seabra in the south-central region of the Brazilian state of Bahia was carried out between 2008 and 2013. Natural infection by Trypanosoma cruzi was evaluated in the specimens collected to monitor vectors of Chagas disease. A total of 1,357 specimens were collected, and four species were identified: Triatoma sordida (83%), Triatoma lenti (16.4%), Triatoma pseudomaculata (0.5%), and Panstrongylus geniculatus (0.1%). Triatoma sordida was found in four cities, only 0.7% in intradomiciliary environments. Triatoma lenti was found only in Macaúbas; 8.5% were found in intradomiciliary environments, 88.3% in peridomiciliary environments, and 3.1% in sylvatic environments. Natural infection by T. cruzi was 0.5% for T. sordida and 3.1% T. lenti. All of these cases were found in peridomiciliary environments of Macaúbas. As the results show, triatomines were found in intradomiciliary environments in three cities that were surveyed in the south-central region of the state of Bahia. Thus, an epidemiologic survey should be performed to avoid the risk of transmission to the population.

  17. Revis\\~ao da Constru\\c{c}\\~ao de Modelos Supersim\\'etricos

    CERN Document Server

    Rodríguez, M C

    2001-01-01

    Foi com base neste estudo que fizemos a constru\\c{c}\\~ao da vers\\~ao supersim\\'etrica dos modelos de simetria $SU(3)_{C} \\otimes SU(3)_{L} \\otimes U(1)_{N}$ \\cite{susy331}, apresentado no final da minha tese de doutorado \\cite{mcr1}. Bem como dos estudos fenomenol\\'ogicos subsequente \\cite{mcr}.

  18. Into the Blue: AO Science with MagAO in the Visible

    CERN Document Server

    Close, Laird M; Follette, Katherine B; Hinz, Phil; Morzinski, Katie M; Wu, Ya-Lin; Kopon, Derek; Riccardi, Armando; Esposito, Simone; Puglisi, Alfio; Pinna, Enrico; Xompero, Marco; Briguglio, Runa; Quiros-Pacheco, Fernando

    2014-01-01

    We review astronomical results in the visible ({\\lambda}<1{\\mu}m) with adaptive optics. Other than a brief period in the early 1990s, there has been little astronomical science done in the visible with AO until recently. The most productive visible AO system to date is our 6.5m Magellan telescope AO system (MagAO). MagAO is an advanced Adaptive Secondary system at the Magellan 6.5m in Chile. This secondary has 585 actuators with < 1 msec response times (0.7 ms typically). We use a pyramid wavefront sensor. The relatively small actuator pitch (~23 cm/subap) allows moderate Strehls to be obtained in the visible (0.63-1.05 microns). We use a CCD AO science camera called "VisAO". On-sky long exposures (60s) achieve <30mas resolutions, 30% Strehls at 0.62 microns (r') with the VisAO camera in 0.5" seeing with bright R < 8 mag stars. These relatively high visible wavelength Strehls are made possible by our powerful combination of a next generation ASM and a Pyramid WFS with 378 controlled modes and 1000...

  19. Twelve thousand laser-AO observations: first results from the Robo-AO large surveys

    Science.gov (United States)

    Law, Nicholas M.; Baranec, Christoph; Riddle, Reed L.

    2014-07-01

    Robo-AO is the first AO system which can feasibly perform surveys of thousands of targets. The system has been operating in a fully robotic mode on the Palomar 1.5m telescope for almost two years. Robo-AO has completed nearly 12,000 high-angular-resolution observations in almost 20 separate science programs including exoplanet characterization, field star binarity, young star binarity and solar system observations. We summarize the Robo-AO surveys and the observations completed to date. We also describe the data-reduction pipeline we developed for Robo-AO—the first fully-automated AO data-reduction, point-spread-function subtraction and companion-search pipeline.

  20. Domestic animal hosts strongly influence human-feeding rates of the Chagas disease vector Triatoma infestans in Argentina.

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    Ricardo E Gürtler

    Full Text Available BACKGROUND: The host species composition in a household and their relative availability affect the host-feeding choices of blood-sucking insects and parasite transmission risks. We investigated four hypotheses regarding factors that affect blood-feeding rates, proportion of human-fed bugs (human blood index, and daily human-feeding rates of Triatoma infestans, the main vector of Chagas disease. METHODS: A cross-sectional survey collected triatomines in human sleeping quarters (domiciles of 49 of 270 rural houses in northwestern Argentina. We developed an improved way of estimating the human-feeding rate of domestic T. infestans populations. We fitted generalized linear mixed-effects models to a global model with six explanatory variables (chicken blood index, dog blood index, bug stage, numbers of human residents, bug abundance, and maximum temperature during the night preceding bug catch and three response variables (daily blood-feeding rate, human blood index, and daily human-feeding rate. Coefficients were estimated via multimodel inference with model averaging. FINDINGS: Median blood-feeding intervals per late-stage bug were 4.1 days, with large variations among households. The main bloodmeal sources were humans (68%, chickens (22%, and dogs (9%. Blood-feeding rates decreased with increases in the chicken blood index. Both the human blood index and daily human-feeding rate decreased substantially with increasing proportions of chicken- or dog-fed bugs, or the presence of chickens indoors. Improved calculations estimated the mean daily human-feeding rate per late-stage bug at 0.231 (95% confidence interval, 0.157-0.305. CONCLUSIONS AND SIGNIFICANCE: Based on the changing availability of chickens in domiciles during spring-summer and the much larger infectivity of dogs compared with humans, we infer that the net effects of chickens in the presence of transmission-competent hosts may be more adequately described by zoopotentiation than by

  1. Phylogeographic pattern and extensive mitochondrial DNA divergence disclose a species complex within the Chagas disease vector Triatoma dimidiata.

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    Fernando A Monteiro

    Full Text Available BACKGROUND: Triatoma dimidiata is among the main vectors of Chagas disease in Latin America. However, and despite important advances, there is no consensus about the taxonomic status of phenotypically divergent T. dimidiata populations, which in most recent papers are regarded as subspecies. METHODOLOGY AND FINDINGS: A total of 126 cyt b sequences (621 bp long were produced for specimens from across the species range. Forty-seven selected specimens representing the main cyt b clades observed (after a preliminary phylogenetic analysis were also sequenced for an ND4 fragment (554 bp long and concatenated with their respective cyt b sequences to produce a combined data set totalling 1175 bp/individual. Bayesian and Maximum-Likelihood phylogenetic analyses of both data sets (cyt b, and cyt b+ND4 disclosed four strongly divergent (all pairwise Kimura 2-parameter distances >0.08, monophyletic groups: Group I occurs from Southern Mexico through Central America into Colombia, with Ecuadorian specimens resembling Nicaraguan material; Group II includes samples from Western-Southwestern Mexico; Group III comprises specimens from the Yucatán peninsula; and Group IV consists of sylvatic samples from Belize. The closely-related, yet formally recognized species T. hegneri from the island of Cozumel falls within the divergence range of the T. dimidiata populations studied. CONCLUSIONS: We propose that Groups I-IV, as well as T. hegneri, should be regarded as separate species. In the Petén of Guatemala, representatives of Groups I, II, and III occur in sympatry; the absence of haplotypes with intermediate genetic distances, as shown by multimodal mismatch distribution plots, clearly indicates that reproductive barriers actively promote within-group cohesion. Some sylvatic specimens from Belize belong to a different species - likely the basal lineage of the T. dimidiata complex, originated ~8.25 Mya. The evidence presented here strongly supports the proposition

  2. Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata

    Science.gov (United States)

    Monteiro, Fernando A.; Peretolchina, Tatiana; Lazoski, Cristiano; Harris, Kecia; Dotson, Ellen M.; Abad-Franch, Fernando; Tamayo, Elsa; Pennington, Pamela M.; Monroy, Carlota; Cordon-Rosales, Celia; Salazar-Schettino, Paz Maria; Gómez-Palacio, Andrés; Grijalva, Mario J.; Beard, Charles B.; Marcet, Paula L.

    2013-01-01

    Background Triatoma dimidiata is among the main vectors of Chagas disease in Latin America. However, and despite important advances, there is no consensus about the taxonomic status of phenotypically divergent T. dimidiata populations, which in most recent papers are regarded as subspecies. Methodology and Findings A total of 126 cyt b sequences (621 bp long) were produced for specimens from across the species range. Forty-seven selected specimens representing the main cyt b clades observed (after a preliminary phylogenetic analysis) were also sequenced for an ND4 fragment (554 bp long) and concatenated with their respective cyt b sequences to produce a combined data set totalling 1175 bp/individual. Bayesian and Maximum-Likelihood phylogenetic analyses of both data sets (cyt b, and cyt b+ND4) disclosed four strongly divergent (all pairwise Kimura 2-parameter distances >0.08), monophyletic groups: Group I occurs from Southern Mexico through Central America into Colombia, with Ecuadorian specimens resembling Nicaraguan material; Group II includes samples from Western-Southwestern Mexico; Group III comprises specimens from the Yucatán peninsula; and Group IV consists of sylvatic samples from Belize. The closely-related, yet formally recognized species T. hegneri from the island of Cozumel falls within the divergence range of the T. dimidiata populations studied. Conclusions We propose that Groups I–IV, as well as T. hegneri, should be regarded as separate species. In the Petén of Guatemala, representatives of Groups I, II, and III occur in sympatry; the absence of haplotypes with intermediate genetic distances, as shown by multimodal mismatch distribution plots, clearly indicates that reproductive barriers actively promote within-group cohesion. Some sylvatic specimens from Belize belong to a different species – likely the basal lineage of the T. dimidiata complex, originated ∼8.25 Mya. The evidence presented here strongly supports the proposition that T

  3. Estados Unidos: do Iraque ao Brasil

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    Virgílio Caixeta Arraes

    2010-12-01

    Full Text Available O presente artigo busca analisar os recentes temas recorrentes na política externa norte-americana, caracterizada pela “guerra contra o terror”, e seus impactos para a agenda a ser discutida na vindoura visita do presidente dos Estados Unidos, Geoge W. Bush, ao Brasil.

  4. Estudos anatômicos e histológicos sôbre a subfamília Triatominae (Heteroptera, Reduviidae: VII. Estudo anatômico do ducto intestinal do Triatoma infestans

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    Dyrce Lacombe

    1957-05-01

    Full Text Available Devido à imprtãncia que certos Triatomíneos hematófagos representam na vida humana, continuarmos a série de estudos já iniciados em nosso laboratório sôbre seus organismos. É feito, no presente trabalho, a anatomia e microanatomai do aparelho digestivo de Triatoma infestans. Das três distintas regiões do duto intestinal estomodeo, mesêntero e proctodeo, a primeira e a terceira são de origem ectodérmica. A região do estomodeo é constituída pela faringe e esôfago; a do proctodeo pelo piloro, íleo e reto. A segunda, de origem endodérmica, consta promesêntero, postmesêntero e da zona de transição. A anatomia e a microanatomia do faringe já foi estudada minuciosamente por BARTH (1952. O esôfago possui numerosas dobras no seu interior revestida de fina cutícula. A musculatura longitudinal e circular acham-se representadas por feixes que, provàvelmente, trabalham, preistàlticamente, transportando o alimento. Não encontramos um proventrículo, de maneira que o esôfago está ligado diretamente à primeira parte endodérmica, isto é, ao promesêntero. No início do promesêntero existe a válvula cardíaca, que, juntamente com as dobras do fim do esôfago, impedem que haja um refluxo do alimento. Durante a alimentação, a parede do preomesêntero, que apresenta numerosas dobras, sofre uma dilatação, a fim de reter u'a maior quantidade de sangue. Entre promesêntero e postmesêntero há um pequeno esfíncter formado pelo aumento da musculatura e das dobras do epitélio. O postmesêntero alcança cinco vêzes mais que o tamanho total do corpo do inseto, e dispõe no abdome em curvas completas, que, muitas vêzes, se superpõem. O seu epitélio possue célula altas e estreitas, e forma muitas dobras para dentro do seu lume. Na parte apical as células possuem um rabdório. O postmesêntero termina após a válvula cardíaca situada atrás do desembocamento das quatro ampolas dos tubos de Malpighi. Entre postmesêntero e

  5. The Subaru Coronagraphic Extreme AO Project

    Science.gov (United States)

    Martinache, Frantz; Guyon, O.; Lozi, J.; Tamura, M.; Hodapp, K.; Suzuki, R.; Hayano, Y.; McElwain, M. W.

    2009-01-01

    While the existence of large numbers of extrasolar planets around solar type stars has been unambiguously demonstrated by radial velocity, transit and microlensing surveys, attempts at direct imaging with AO-equipped large telescopes remain unsuccessful. Because they supposedly offer more favorable contrast ratios, young systems consitute prime targets for imaging. Such observations will provide key insights on the formation and early evolution of planets and disks. Current surveys are limited by modest AO performance which limits inner working angle to 0.2", and only reach maximum sensitivity outside 1". This translates into orbital distances greater than 10 AU even on most nearby systems, while only 5 % of the known exoplanets have a semimajor axis greater than 10 AU. This calls for a major change of approach in the techniques used for direct imaging of the direct vicinity of stars. A sensible way to do the job is to combine coronagraphy and Extreme AO. Only accurate and fast control of the wavefront will permit the detection of high contrast planetary companions within 10 AU. The SCExAO system, currently under assembly, is an upgrade of the HiCIAO coronagraphic differential imaging camera, mounted behind the 188-actuator curvature AO system on Subaru Telescope. This platform includes a 1000-actuator MEMS deformable mirror for high accuracy wavefront correction and a PIAA coronagraph which delivers high contrast at 0.05" from the star (5 AU at 100 pc). Key technologies have been validated in the laboratory: high performance wavefront sensing schemes, spider vanes and central obstruction removal, and lossless beam apodization. The project is designed to be highly flexible to continuously integrate new technologies with high scientific payoff. Planned upgrades include an integral field unit for spectral characterization of planets/disks and a non-redundant aperture mask to push the performance of the system toward separations less than lambda/D.

  6. Role of two Triatoma (Hemiptera: Reduviidae: Triatominae) species in the transmission of Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) to man in the west coast of Mexico.

    Science.gov (United States)

    Martínez-Ibarra, J A; Bárcenas-Ortega, N M; Nogueda-Torres, B; Alejandre-Aguilar, R; Lino Rodríguez, M; Magallón-Gastélum, E; López-Martínez, V; Romero-Nápoles, J

    2001-02-01

    From August 1997 to August 1998, 334 specimens of Triatoma longipennis and 62 of T. picturata were collected in four groups of localities placed in the zone from Guadalajara, Jalisco to Tepic, Nayarit, in the West Coast of Mexico. Most T. longipennis were collected outdoors (69.2%) while most T. picturata (58.1%) were collected indoors. All collected specimens were examined for Trypanosoma cruzi infection, which was detected on 98 (29.3%) T. longipennis and 17 (27.4%) T. picturata. This study confirms the role of T. longipennis and T. picturata as some of the main T. cruzi vectors to humans in Mexico. Habitation Infestation Rate with T. longipennis was of 0.09 and with T. picturata was of 0.03 and the predominating ecotopes were pile of blocks, chicken coops, pigsties, wall crawls and beds.

  7. Role of two Triatoma (Hemiptera: Reduviidae: Triatominae species in the transmission of Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae to man in the West Coast of Mexico

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    José Alejandro Martínez-Ibarra

    2001-02-01

    Full Text Available From August 1997 to August 1998, 334 specimens of Triatoma longipennis and 62 of T. picturata were collected in four groups of localities placed in the zone from Guadalajara, Jalisco to Tepic, Nayarit, in the West Coast of Mexico. Most T. longipennis were collected outdoors (69.2% while most T. picturata (58.1% were collected indoors. All collected specimens were examined for Trypanosoma cruzi infection, which was detected on 98 (29.3% T. longipennis and 17 (27.4% T. picturata. This study confirms the role of T. longipennis and T. picturata as some of the main T. cruzi vectors to humans in Mexico. Habitation Infestation Rate with T. longipennis was of 0.09 and with T. picturata was of 0.03 and the predominating ecotopes were pile of blocks, chicken coops, pigsties, wall crawls and beds.

  8. Entomological indices, feeding sources, and molecular identification of Triatoma phyllosoma (Hemiptera: Reduviidae) one of the main vectors of Chagas disease in the Istmo de Tehuantepec, Oaxaca, Mexico.

    Science.gov (United States)

    Villalobos, Guiehdani; Martínez-Hernández, Fernando; de la Torre, Patricia; Laclette, Juan Pedro; Espinoza, Bertha

    2011-09-01

    The purpose of this study was to conduct an entomological analysis, determination of feeding sources, and molecular identification of triatomines in five communities of the Istmo de Tehuantepec, Oaxaca. The only found species in two of five searched communities (San Mateo del Mar and Tehuantepec City) was Triatoma phyllosoma. Colonization indices were high in both communities. In San Mateo del Mar, the insects were found indoors and in Tehuantepec City in peridomestic areas. The Trypanosoma cruzi infection indices were 2.1% in San Mateo del Mar and 39.4% in Tehuantepec City. This difference could be related to the high numbers of triatomine feeding on hens in the former community. In contrast, in Tehuantepec, dogs were the principal triatomine feeding sources. All nymphs and adults that were genetically analyzed belonged to the species T. phyllosoma. Low levels of genetic variation were found between vectors from both communities.

  9. Cytogenetic and morphologic approaches of hybrids from experimental crosses between Triatoma lenti Sherlock & Serafim, 1967 and T. sherlocki Papa et al., 2002 (Hemiptera: Reduviidae).

    Science.gov (United States)

    Mendonça, Vagner José; Alevi, Kaio Cesar Chaboli; Medeiros, Lívia Maria de Oliveira; Nascimento, Juliana Damieli; de Azeredo-Oliveira, Maria Tercília Vilela; da Rosa, João Aristeu

    2014-08-01

    The reproductive capacity between Triatoma lenti and Triatoma sherlocki was observed in order to verify the fertility and viability of the offspring. Cytogenetic, morphological and morphometric approaches were used to analyze the differences that were inherited. Experimental crosses were performed in both directions. The fertility rate of the eggs in crosses involving T. sherlocki females was 65% and 90% in F1 and F2 offspring, respectively. In reciprocal crosses, it was 7% and 25% in F1 and F2 offspring, respectively. The cytogenetic analyses of the male meiotic process of the hybrids were performed using lacto-acetic orcein, C-banding and Feulgen techniques. The male F1 offspring presented normal chromosome behavior, a finding that was similar to those reported in parental species. However, cytogenetic analysis of F2 offspring showed errors in chromosome pairing. This post-zygotic isolation, which prevents hybrids in nature, may represent the collapse of the hybrid. This phenomenon is due to a genetic dysregulation that occurs in the chromosomes of F1. The results were similar in the hybrids from both crosses. Morphological features, such as color and size of connexive and the presence of red-orange rings on the femora, were similar to T. sherlocki, while wins size was similar to T. lenti in F1 offspring. The eggshells showed characteristics that were similar to species of origin, whereas the median process of the pygophore resulted in intermediate characteristics in the F1 and a segregating pattern in F2 offspring. Geometric morphometric techniques used on the wings showed that both F1 and F2 offspring were similar to T. lenti. These studies on the reproductive capacity between T. lenti and T. sherlocki confirm that both species are evolutionarily closed; hence, they are included in the brasiliensis subcomplex. The extremely reduced fertility observed in the F2 hybrids confirmed the specific status of the species that were analyzed.

  10. Perfil alimentario de Triatoma rubrovaria (Blanchard, 1843 (Hemiptera, Triatominae en ámbitos peridomiciliarios, de una localidad rural de Uruguay Feeding patterns of Triatoma rubrovaria (Blanchard, 1843 (Hemiptera, Triatominae in peridomestic habitats, in a rural area of Uruguay

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    Roberto Salvatella

    1994-08-01

    Full Text Available En una localidad con población rural dispersa ("La Bolsa", 3ª seccional, dpto. de Artigas, Uruguay se estudió, en ámbitos peridomiciliarios, el perfil alimentario de T. rubrovaria (triatomíneo silvestre y potencial vector secundario de colonización intradomiciliaria, utilizándose la técnica de doble difusión en agar, enfrentando contenido promesentérico frente a un panel de 13 sueros. Se pudo detectar en 120 insectos 251 identificaciones de fuente hematofágica con alimentación predominante en mamíferos (73%, pero marcado eclectismo alimentario (mamíferos, aves, reptiles y cucarachas, incluyendo hematofagia sobre seres humanos en un 8% de las identificaciones totales. La micropredación de hemolinfa lo ubicaría en una situación evolutiva primitiva, intermedia entre predator/entomófago y triatomineo/hematófago, que comparte con T. circunmaculata. Los mamíferos detectados con mayor frecuencia fueron dasipódidos y bóvidos, aunque la fuente hematofágica, salvo en los adultos alados, se constituye en un fenómeno de proximidad ocasional por cohabitación de un mismo habitat. La frecuencia de alimentación sobre hombre, hallada en un ambiente peridomiciliario, aporta un importante elemento a su capacidad vectorial potencial. En el análisis espacial de las dietas se muestra al peridomicilio como un área de interacción de hospederos domésticos, silvestres y sinantrópicos. La infección tripanosómica fue mínima comparada con las altas tasas de infección de ámbitos silvestres.It has been studied the feeding patterns of Triatoma rubrovaria (wild triatominae bug, and intradomiciliary secondary vector of T. cruzi in a rural area of "La Bolsa" (Department of Artigas. Agar double diffusion test were used for analyze the blood meal, from 120 insects, which were confronted to 13 antisera. It has 251 identify blood meals, and the most frequently was mamalian host (73%, but it has a variable source of feeding (mammals, birds, reptils

  11. Labirintite consequente ao uso de difenilhidantoina

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    Eneida Baptistete Matarazzo

    1978-03-01

    Full Text Available É relatado o caso de paciente epilético que, aos 16 anos de idade, ao fazer uso de difenilhidantoina, apresentou, já nos primeiros dias, sinais de intolerância, com sensação de formigamento das extremidades. Aos 36 anos voltou a fazer uso desse mesmo medicamento e apresentou sintomas de labirintite, tendo crises de tipo rotatório quando inclinava a cabeça para a esquerda ou a movia bruscamente. O tratamento específico para labirintite, orientado por otorrinolaringologista, foi ineficaz e o quadro clínico agravou-se progressivamente. Ao fim de seis meses seu exame psíquico revelava leve torpor, lentificação do raciocínio, incapacidade para o trabalho e depressão reativa, observando-se, do ponto de vista neurológico, discreto desvio da marcha para a esquerda e discreto aumento da base de sustentação. Além disto referia a existência de crises rotatórias e vômitos tão frequentes que nos últimos dois meses precisou ser hospitalizado algumas vezes, para ser hidratado. A suspensão da difenilhidantoina com manutenção exclusiva de fenobarbital (do qual também já vinha fazendo uso, determinou regressão rápida dos sintomas citados. Tal fato permitiu concluir que a labirintite foi provocada pelo uso da difenilhidantoina e deve ser incluida na extensa relação de efeitos colaterais provocados por essa droga.

  12. Alguns aspectos da biologia de Triatoma pseudomaculata Corrêa & Espínola, 1964, em condições de laboratório (Hemiptera:Reduviidae:Triatominae

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    Teresa Cristina M Gonçalves

    1997-03-01

    Full Text Available Biology of Triatoma pseudomaculata Corrêa & Espínola, 1964, under Laboratory Conditions (Hemiptera:Reduviidae:Triatominae - Observations were made on the evolutive cycle of Triatoma pseudomaculata, held under laboratory conditions, fed weekly on bird (pigeon. Of 60 eggs obtained, only 34 nymphs reached the adult stage in a period of X(S=398±76 days. The following parameters were observed: the time immature stages took to develop from egg to adult emergence; the occurrence of the first meal; the time-lapse between the presenting of the blood-meal and the begining of feeding; time of feeding; amount of blood ingested; variation of weight 24 hr after the blood-meal and until the next blood-meal; and the defecation pattern. The experiment was carried out for 20 months, held in BOD incubator with the average of temperature and humidity of 28±1ºC and 80±5% RU, respectively

  13. Abordagens ao aprendizado político

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    Krischke, Paulo J.

    2002-01-01

    Full Text Available Há um reconhecimento crescente entre os cientistas políticos de que uma fonte principal para a conquista e o aperfeiçoamento da democracia é o aprendizado político por parte dos atores sociais e políticos. Contudo, a bibliografia especializada não considera satisfatoriamente como o aprendizado político reorienta os comportamentos e atitudes em apoio à democracia política e seu aperfeiçoamento. A presente proposta acata esse desafio, numa revisão teórica inicial das abordagens existentes ao aprendizado político no contexto da globalização. Num primeiro momento, considera as limitações da abordagem ao estudo das elites nos processos de democratização. Em segundo lugar salienta as dificuldades da teoria da modernização para enfrentar o tema, nos estudos sobre os públicos massivos. Mais adiante sugere as contribuições que uma abordagem inspirada nos trabalhos de Jürgen Habermas pode proporcionar ao estudo do aprendizado político. E conclui indicando certas interfaces temáticas a serem examinadas na bibliografia no contexto da globalização. À luz de resultados de uma pesquisa atualmente em sua fase final

  14. Esta é minha carta ao mundo

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    Fernanda Mourão

    2009-01-01

    Full Text Available A partir da primeira carta de Emily Dickinson (1830-1886 a Thomas Higginson, que então seria seu “preceptor” e interlocutor para sempre, este texto propõe uma leitura da obra da escritora norte-americana a partir do biografema da carta e da ideia de sua obra como “carta ao mundo” – conforme um de seus mais famosos poemas –, com todas as implicações trazidas pelo termo, e à luz das teorias de Roland Barthes, Maurice Blanchot e Silvina Rodrigues Lopes, entre outros.

  15. AO corrected satellite imaging from Mount Stromlo

    Science.gov (United States)

    Bennet, F.; Rigaut, F.; Price, I.; Herrald, N.; Ritchie, I.; Smith, C.

    2016-07-01

    The Research School of Astronomy and Astrophysics have been developing adaptive optics systems for space situational awareness. As part of this program we have developed satellite imaging using compact adaptive optics systems for small (1-2 m) telescopes such as those operated by Electro Optic Systems (EOS) from the Mount Stromlo Observatory. We have focused on making compact, simple, and high performance AO systems using modern high stroke high speed deformable mirrors and EMCCD cameras. We are able to track satellites down to magnitude 10 with a Strehl in excess of 20% in median seeing.

  16. Nefrocalcinose associada ao uso de esteroide anabolizante

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    Weverton Machado Luchi

    2015-03-01

    Full Text Available Os esteroides anabolizantes têm sido usados como arma terapêutica em diversas condições clínicas. Entretanto, o uso abusivo e indiscriminado, associado a outros suplementos nutricionais, tem gerado efeitos adversos graves. Relato do caso: Sexo masculino, 21 anos, admitido com náuseas, astenia, hiporexia, cefaleia e hipertensão arterial. Exames no sangue evidenciaram Cr: 3,9 mg/dl U:100 mg/dl e Cálcio total 14 mg/dl. Ultrassonografia e biópsia renal compatíveis com nefrocalcinose. Houve melhora gradativa da função renal e da calcemia após hidratação vigorosa e furosemida. Entretanto, após 1 ano, persistiram depósitos renais de cálcio e relação córticomedular reduzida ao ultrassom e creatinina estável em 1,4 mg/dl. Casos anteriores evidenciaram necrose tubular aguda e nefrite intersticial com poucos depósitos de cálcio no interstício renal. Nesse caso, encontramos nefrocalcinose acentuada associada à nefroesclerose. O objetivo deste estudo é relatar a ocorrência de injúria renal aguda com nefrocalcinose associada ao uso de esteroide anabolizante e oferecer uma revisão do assunto.

  17. Diferentes Metodologias Aplicadas ao Ensino de Astronomia

    Science.gov (United States)

    Albrecht, E.; Voelzke, M. R.

    2007-08-01

    Espera-se que o educando ao final da educação básica, adquira uma compreensão atualizada das hipóteses, modelos e formas de investigação sobre a origem e evolução do Universo em que vive. O presente trabalho tem como principal objetivo compreender dentre três práticas pedagógicas adotadas no Ensino de Astronomia, na terceira série do Ensino Médio, da Escola Estadual Colônia dos Pescadores, qual melhor cumpre o papel de formação e aprendizagem para vida. A pesquisa preliminar foi através de um questionário onde o intuito foi diagnosticar o conhecimento já existente acerca do tema em questão. O questionário é composto de vinte questões dissertativas e objetivas, onde os educandos das três turmas envolvidas o responderam. Este trabalho utiliza as seguintes metodologias: a tradicional, onde o professor é um repassador de informações, fazendo uso exclusivo de lousa e giz; a segunda também de forma tradicional, porém com auxílio de multimídia para desenvolvimento das aulas e aterceira sob forma de seminários, elaborados e apresentados pelos educandos, no qual o educador faz apenas as intervenções necessárias. Ao final do trabalho os alunos responderão novamente o questionário inicial para diagnosticar dentre as três metodologias utilizadas qual apresentou melhor resultado. Os resultados preliminares obtidos, já podem ser observados e, dos 119 alunos entrevistados, as respostas obtidas são as mais diversas e evidenciam que a grande maioria nunca teve em sua vida escolar o tema Astronomia. Ao serem questionados se já haviam estudado Astronomia as respostas foram: turma A: sim 43%; turma B: sim: 21%; turma C: sim: 24%. Porém quando questionados a respeito do significado de Astronomia observou-se que: turma A: 100% de acertos; turma B: 64% acertos; turma C: 84% de acertos, demonstrando claramente a aprendizagem em diferentes esferas, não dependendo unicamente da escola. Até o presente momento, verificou-se que há interesse em

  18. Southern Cone Initiative for the elimination of domestic populations of Triatoma infestans and the interruption of transfusional Chagas disease. Historical aspects, present situation, and perspectives.

    Science.gov (United States)

    Dias, João Carlos Pinto

    2007-10-30

    Created in 1991 by the governments of Argentina, Bolivia, Brazil, Chile, Paraguay, and Uruguay, the Southern Cone Initiative (SCI) has been extremely important for Chagas disease control in this region. Its basic objective was to reach the interruption of this disease, chiefly by means of the elimination of the principal vector Triatoma infestans and by the selection of safe donors in the regional blood banks. After a summarized historic of SCI, the text shows the advance of technical and operative activities, emphasizing some factors for the initiative success, as well as some difficulties and constraints. The future of SCI will depend of the continuity of the actions and of political priority. Scientific community has been highly responsible for this initiative and its maintenance. At the side of this, national and international efforts must be involved and reinforced to assure the accomplishment of the final targets of SCI. Very specially, the Pan American Health Organization has cooperated with the Initiative in all its moments and activities,being the most important catalytic and technical factor for SCI success.

  19. Is imidacloprid an effective alternative for controlling pyrethroid-resistant populations of Triatoma infestans (Hemiptera: Reduviidae in the Gran Chaco ecoregion?

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    Guillermo Carvajal

    2014-09-01

    Full Text Available The prevention of Chagas disease is based primarily on the chemical control of Triatoma infestans (Klug using pyrethroid insecticides. However, high resistance levels, correlated with control failures, have been detected in Argentina and Bolivia. A previous study at our laboratory found that imidacloprid could serve as an alternative to pyrethroid insecticides. We studied the delayed toxicity of imidacloprid and the influence of the blood feeding condition of the insect on the toxicity of this insecticide; we also studied the effectiveness of various commercial imidacloprid formulations against a pyrethroid-resistant T. infestans population from the Gran Chaco ecoregion. Variations in the toxic effects of imidacloprid were not observed up to 72 h after exposure and were not found to depend on the blood feeding condition of susceptible and resistant individuals. Of the three different studied formulations of imidacloprid on glass and filter paper, only the spot-on formulation was effective. This formulation was applied to pigeons at doses of 1, 5, 20 and 40 mg/bird. The nymphs that fed on pigeons treated with 20 mg or 40 mg of the formulation showed a higher mortality rate than the control group one day and seven days post-treatment (p < 0.01. A spot-on formulation of imidacloprid was effective against pyrethroid-resistant T. infestans populations at the laboratory level.

  20. Is imidacloprid an effective alternative for controlling pyrethroid-resistant populations of Triatoma infestans (Hemiptera: Reduviidae) in the Gran Chaco ecoregion?

    Science.gov (United States)

    Carvajal, Guillermo; Picollo, María Inés; Toloza, Ariel Ceferino

    2014-09-01

    The prevention of Chagas disease is based primarily on the chemical control of Triatoma infestans (Klug) using pyrethroid insecticides. However, high resistance levels, correlated with control failures, have been detected in Argentina and Bolivia. A previous study at our laboratory found that imidacloprid could serve as an alternative to pyrethroid insecticides. We studied the delayed toxicity of imidacloprid and the influence of the blood feeding condition of the insect on the toxicity of this insecticide; we also studied the effectiveness of various commercial imidacloprid formulations against a pyrethroid-resistant T. infestans population from the Gran Chaco ecoregion. Variations in the toxic effects of imidacloprid were not observed up to 72 h after exposure and were not found to depend on the blood feeding condition of susceptible and resistant individuals. Of the three different studied formulations of imidacloprid on glass and filter paper, only the spot-on formulation was effective. This formulation was applied to pigeons at doses of 1, 5, 20 and 40 mg/bird. The nymphs that fed on pigeons treated with 20 mg or 40 mg of the formulation showed a higher mortality rate than the control group one day and seven days post-treatment (p imidacloprid was effective against pyrethroid-resistant T. infestans populations at the laboratory level.

  1. L925I mutation in the Para-type sodium channel is associated with pyrethroid resistance in Triatoma infestans from the Gran Chaco region.

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    Natalia Capriotti

    Full Text Available BACKGROUND: Chagas' disease is an important public health concern in Latin America. Despite intensive vector control efforts using pyrethroid insecticides, the elimination of Triatoma infestans has failed in the Gran Chaco, an ecoregion that extends over Argentina, Paraguay, Bolivia and Brazil. The voltage-gated sodium channel is the target site of pyrethroid insecticides. Point mutations in domain II region of the channel have been implicated in pyrethroid resistance of several insect species. METHODS AND FINDINGS: In the present paper, we identify L925I, a new pyrethroid resistance-conferring mutation in T. infestans. This mutation has been found only in hemipterans. In T. infestans, L925I mutation occurs in a resistant population from the Gran Chaco region and is associated with inefficiency in the control campaigns. We also describe a method to detect L925I mutation in individuals from the field. CONCLUSIONS AND SIGNIFICANCE: The findings have important implications in the implementation of strategies for resistance management and in the rational design of campaigns for the control of Chagas' disease transmission.

  2. Eco-epidemiology of Chagas disease in northeastern Brazil: Triatoma brasiliensis, T. pseudomaculata and Rhodnius nasutus in the sylvatic, peridomestic and domestic environments.

    Science.gov (United States)

    Sarquis, Otília; Carvalho-Costa, Filipe Anibal; Toma, Helena Keiko; Georg, Ingebourg; Burgoa, Marcelo R; Lima, Marli Maria

    2012-04-01

    An entomological survey was carried out in four rural localities situated in the state of Ceará, assessing Chagas disease seroprevalence in man, focusing on the presence of vectors in natural foci contiguous to the domestic and peridomestic environments. Fifty-three Triatoma brasiliensis, nine T. pseudomaculata and 71 Rhodnius nasutus were collected in their natural habitats as far as 10 m from the houses, and 663, 59 and 8 respectively were captured in peridomestic artificial structures, adjacent to the houses, including henhouses, pigpens, corrals, perches and piles of bricks, tiles and wood. Within the households, 37 T. brasiliensis, one specimen of T. pseudomaculata and one of R. nasutus were captured. Overall, Trypanosoma cruzi infection rates were 2.3% for T. brasiliensis and 11.3% for R. nasutus. Despite that the seroprevalence survey in man did not reveal positive results using two serological techniques, natural triatomine habitats are juxtaposed to man-made artificial ecotopes, resulting in overlapping habitats. The contiguity between natural ecotopes and human dwellings increases the interaction between vectors and humans, challenging continuous surveillance and vector control efforts.

  3. Composition and anti-insect activity of essential oils from Tagetes L. species (Asteraceae, Helenieae) on Ceratitis capitata Wiedemann and Triatoma infestans Klug.

    Science.gov (United States)

    López, Sandra B; López, María L; Aragón, Liliana M; Tereschuk, María L; Slanis, Alberto C; Feresin, Gabriela E; Zygadlo, Julio A; Tapia, Alejandro A

    2011-05-25

    Essential oils from four species of the genus Tagetes L. (Asteraceae, Helenieae) collected in Tucumán province, Argentina, were evaluated for their chemical composition, toxicity, and olfactory activity on Mediterranean fruit fly Ceratitis capitata Wiedemann adults and for repellent properties on Triatoma infestans (Klug) (Chagas disease vector). Yields of essential oils range from 0.2 to 0.8% (v/w). The same main constituents among Tagetes minuta L., Tagestes rupestris Cabrera, and Tagetes terniflora Kunth, (cis-trans)-ocimenes, (cis-trans)-tagetones, and (cis-trans)-ocimenones showed important differences in their relative compositions. Tagetes filifolia Lag. was characterized by the recognized phenylpropanoids methylchavicol and trans-anethole as the main components. LD(50) was ≤20 μg/insect in topical bioassays. T. rupestris was the most toxic to C. capitata females, whereas the other oils presented similar toxicities against males and females. Tagetes rupestris oil attracted both sexes of C. capitata at 5 μg, whereas T. minuta showed opposite activities between males (attractant) and females (repellent). Oils from T. minuta and T. filifolia were the most repellent to T. infestans. The results suggest that compositions of essential oils influence their insecticidal and olfactory properties. The essential oils from Tagetes species show an important potential as infochemical agents on insects' behaviors. This study highlights the chemical variability of essential oils as a source of variation of anti-insect properties.

  4. Comparative study of enzymes in testes and ovaries from adult Dipetalogaster maximus (Uhler and Triatoma infestans (Klug (Hemiptera: Reduviidae. Correlation with fine structural organization

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    PY Scaraffia

    2009-12-01

    Full Text Available Activities of hexokinase (HK, glucose-6-phosphate dehydrogenase (G6PDH, fructose-6-phosphate kinase (F6PK, glutamate dehydrogenase (GlutDH, aspartate aminotransferase (AAT, malate dehydrogenase (MDH and glycerol-3-phosphate dehydrogenase (GPDH were determined in tissue extracts of testes and ovaries of adult Dipetalogaster maximus (Uhler and Triatoma infestans (Klug (Hemiptera: Reduviidae, insect vectors of Chagas disease. The fine structure organization of the same organs were studied by electron microscopy. Results allow the following inferences: in testes from both species, most of the glucose would be utilized through the glycolytic pathway. Amino acid catabolism for energy purposes appears to be unimportant. The number of mitochondria and the development of the rough endoplasmic reticulum in cells of the spermatogenic line indicate the occurrence of active oxidative metabolism and protein synthesis; in ovaries, levels of G6PDH indicate the existence of an active pentose pathway which would supply the NADPH required for fat and ecdysteroid synthesis. Amino acid catabolism appears to be relatively more important in ovary than in testis. Fat and glycogen are stored in follicular cells of D. maximus; oocytes of both species contain numerous fat droplets. Abundant mitocondria are present in follicular cells and oocytes. A well developed rough endoplasmic reticulum and free ribosomes are also conspicuous in these cells. The malate/aspartate H-transfer system seemed to be relatively more important than the glycerophosphate shuttle in ovaries as well in testes.

  5. Insects found in birds' nests from Argentina. Pseudoseisura lophotes Reichenbach, 1853 and Anumbius annumbi (Vieillot, 1817) (Aves: Furnariidae), hosts of Triatoma platensis Neiva, 1913 (Hemiptera: Reduviidae: Triatominae).

    Science.gov (United States)

    Paola, Turienzo

    2014-02-24

    The insect fauna of the nests of Pseudoseisura lophotes (Reichenbach, 1853) (Aves: Furnariidae) from Argentina was investigated. A total of 110 species (68 identified to species, 22 identified to genus, 20 identified to family) in 40 families of 10 orders of insects was found in these nests. Triatoma platensis Neiva, 1913 (Hemiptera: Reduviidae) was found again in nests of P. lophotes, corroborating after 73 years the first observations made by Mazza in 1936. The occurrence of the insects in nests of P. lophotes is compared with the previously known insect fauna in nests of A. annumbi, Furnarius rufus (Furnariidae), and Myiopsitta monachus (Psittacidae). The insect fauna in additional nests of Anumbius annumbi from the same and/or different localities is given, and used in comparisons. The first occurrence of Cuterebridae (Diptera) in birds' nests, their pupae as the overwintering stage, and the second simultaneous infestation by two species of Philornis (Diptera: Muscidae) on the same nestlings are presented. Other simultaneous infestations of different hematophagous arthropods (Hemiptera: Cimidae; Reduviidae: Triatominae, and Acari: Argasidae) are remarked and discussed.

  6. Southern Cone Initiative for the elimination of domestic populations of Triatoma infestans and the interruption of transfusion Chagas disease: historical aspects, present situation, and perspectives

    Directory of Open Access Journals (Sweden)

    João Carlos Pinto Dias

    2007-10-01

    Full Text Available Created in 1991 by the governments of Argentina, Bolivia, Brazil, Chile, Paraguay, and Uruguay, the Southern Cone Initiative (SCI has been extremely important for Chagas disease control in this region. Its basic objective was to reach the interruption of this disease, chiefly by means of the elimination of the principal vector Triatoma infestans and by the selection of safe donors in the regional blood banks. After a summarized historic of SCI, the text shows the advance of technical and operative activities, emphasizing some factors for the initiative success, as well as some difficulties and constraints. The future of SCI will depend of the continuity of the actions and of political priority. Scientific community has been highly responsible for this initiative and its maintenance. At the side of this, national and international efforts must be involved and reinforced to assure the accomplishment of the final targets of SCI. Very specially, the Pan American Health Organization has cooperated with the Initiative in all its moments and activities,being the most important catalytic and technical factor for SCI success.

  7. Ecologia domestica de la tripanosomiasis americana: perfil alimentario del triatoma infestans en un area rural de la província de Santiago del Estero, Argentina

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    Cristina Wisnivesky-Colli

    1987-03-01

    Full Text Available Se describen los resultados entomológicos de un estúdio sobre transmiswn del Tripanosoma cruzi realizado en un caserío rural de Argentina. Se determino la prevalencia de infección elperfil alimentario de los Triatoma infestans domiciliados, relacionándose su densidad con el tipo de vivienda. Seis de las 18 viviendas estudiadas eran nuevas o mejoradas y en 16 los habitantes aplicaban hexaclorociclohexano. La menor densidad de vinchucas correspondió a las casas higiénicas o mejoradas con tratamiento químico, Los ranchos tratados presentaron un número de insectos 10 veces menor que un rancho no tratado, indicando la efectividad del control autogestado. Se hallaron T. infestans en el 100% de los dormitorios pero en sólo el 53% de los localesperidomiciliarios. Un 39% de las vinchucas intradomiciliarias estaban infectadas. El 96% de los insectos de dormitorios se alimentaron sobre hombre, perro, polloygato, siendo el índice afinidad para el perro 13 veces superior al correspondiente al hombre. Se discute la importancia epidemiológica de la concentración de vectores dentro de los dormitorios y su asociación trófica con los perros convivientes, nsí como la necessidad de combinar la mejora de vivienda con educación sanitaria.

  8. Life cycle and reproductive patterns of Triatoma rubrovaria (Blanchard, 1843) (Hemiptera: Reduviidae) under constant and fluctuating conditions of temperature and humidity.

    Science.gov (United States)

    Damborsky, Miryam P; Bar, María E; Gorla, David

    2005-01-01

    The aim of this study was to evaluate the temperature and relative humidity influence in the life cycle, mortality and fecundity patterns of Triatoma rubrovaria. Four cohorts with 60 recently laid eggs each were conformed. The cohorts were divided into two groups. In the controlled conditions group insects were maintained in a dark climatic chamber under constant temperature and humidity, whereas triatomines of the ambiental temperature group were maintained at room temperature. Average incubation time was 15.6 days in the controlled conditions group and 19.1 days in the ambiental temperature. In group controlled conditions the time from egg to adult development lasted 10 months while group ambiental temperature took four months longer. Egg eclosion rate was 99.1% and 98.3% in controlled conditions and ambiental temperature, respectively. Total nymphal mortality in controlled conditions was 52.6% whereas in ambiental temperature was 51.8%. Mean number of eggs/female was 817.6 controlled conditions and 837.1 ambiental temperature. Fluctuating temperature and humidity promoted changes in the life cycle duration and in the reproductive performance of this species, although not in the species mortality.

  9. Triatoma rubrovaria (Blanchard, 1843 (Hemiptera, Reduviidae, Triatominae II: trophic resources and ecological observations of five populations collected in the State of Rio Grande do Sul, Brazil

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    Almeida Carlos Eduardo

    2002-01-01

    Full Text Available Triatoma rubrovaria has become the most frequently captured triatomine species after the control of T. infestans in the State of Rio Grande do Sul (RS, Brazil. Isoenzymatic and chromatic studies indicate the existence of, at least, two distinct phenotypic patterns of T. rubrovaria in RS. The geographic variation noted through molecular tools may also result in distinct profiles of vectorial potentiality. In order to enhance our understanding of the bionomic knowledge of T. rubrovaria separate batches of the species were collected from different municipalities of RS distant from 72 to 332 km: Santana do Livramento (natural ecotope, Santana do Livramento (artificial ecotope, Santiago (natural ecotope, Canguçu (peridomicile and Encruzilhada do Sul (natural ecotope. A total of 285 specimens were collected, 85 specimens kept sufficient fecal material in their guts for the precipitin analysis. The results indicated the food eclecticism for this species and the anti-rodent serum showed the highest positivity in most localities. From the total of analyzed samples, only 1.3% of unique positivity for human blood was registered, all of them for Santiago population. This reactivity to human blood may be associated to pastures activities in the field.

  10. LGS-AO Imaging of Every Kepler Planet Candidate: the Robo-AO KOI Survey

    Science.gov (United States)

    Baranec, Christoph; Law, Nicholas; Morton, Timothy; Ziegler, Carl; Nofi, Larissa; Atkinson, Dani; Riddle, Reed

    2015-12-01

    The Robo-AO Kepler Planetary Candidate Survey is observing every Kepler planet candidate host star with laser adaptive optics imaging, to search for blended nearby stars which may be physically associated companions and/or responsible for transit false positives. We will present the results from searching for companions around over 3,000 Kepler planet hosts in 2012-2015. We will describe our first data release covering 715 planet candidate hosts, and give a preview of ongoing results including improved statistics on the likelihood of false positive planet detections in the Kepler dataset, many new planets in multiple star systems, and new exotic multiple star systems containing Kepler planets. We will also describe the automated Robo-AO survey data reduction methods, including a method of using the large ensemble of target observations as mutual point-spread-function references, along with a new automated companion-detection algorithm designed for extremely large adaptive optics surveys. Our first data release covered 715 objects, searching for companions from 0.15” to 2.5” separation with contrast up to 6 magnitudes. We measured the overall nearby-star-probability for Kepler planet candidates to be 7.4+/-1.0%, and we will detail the variations in this number with stellar host parameters. We will also discuss plans to extend the survey to other transiting planet missions such as K2 and TESS as Robo-AO is in the process of being re-deployed to the 2.1-m telescope at Kitt Peak for 3 years and a higher-contrast Robo-AO system is being developed for the 2.2-m UH telescope on Maunakea.

  11. Review of AO calibrations, or how to best educate your AO system

    Science.gov (United States)

    Kolb, Johann

    2016-07-01

    If the Real-Time Computer is the heart of an AO system, the Wavefront Sensor (WFS) its eyes, the Deformable Mirror (DM) its hands and the control strategy its nervous system, the sum of all those parts is made into a harmonious entity thanks to calibrations. This paper does not have the ambition to provide an overview of all the currently existing calibration strategies, but rather to focus on a few challenging problems and their recent evolution in the era of adaptive telescopes, mostly based on the experience of ESO's Adaptive Optics Instruments in general and the AO Facility in particular. Single most important calibration in post-focal AO system, the recording of the Interaction Matrix (IM) between WFS and DM has since long evolved to use fast modulation techniques, has shown to be feasible on-sky and is now almost free from measurements thanks to its pseudo-synthetic generation, quasi-mandatory solution in an adaptive telescope. Pseudo- because it requires an unprecedented knowledge of the components' characteristics, especially the WFS, DM and the optical registration between the two. Bigger telescopes and the use of Laser Guide Stars (LGS) also mean that the properties of the system will change in time and thus need to be constantly updated thanks to online diagnosis tools for spot size measurement, atmosphere monitoring, Wavefront Sensing and control optimization. New loops come into play like the one to minimize LGS Jitter and the one taking over the telescope active optics by means of offloading the DM low orders, and they all require calibration. More calibration means more time and one has to carefully balance the calibrations that require precious telescope night time, day time or for the best, no telescope time at all. Their importance sometimes underestimated, calibrations have repeatedly shown to be a vital part in the optimum functioning of present and future AO systems.

  12. Adesão ao tratamento da Hanseníase: dificuldades inerentes aos portadores

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    Izaildo Tavares Luna

    2010-12-01

    Full Text Available Esta pesquisa, realizada em Guaiúba, CE, Brasil, objetivou identificar as dificuldades de adesão ao tratamento pelos portadores de hanseníase, relacionando os fatores associados. Estudo qualitativo, cujos dados foram coletados a partir de uma visita domiciliária com roteiro de entrevista contendo sete questões norteadoras. Participaram pacientes com diagnóstico de hanseníase e histórico de não adesão ao tratamento. Utilizou-se o Modelo de Atividade de Vida de Roper, Logan e Tierney. As falas dos sujeitos foram categorizadas de acordo com as doze atividades de vida. A utilização do modelo possibilitou uma abordagem holística sobre as barreiras vivenciadas pelo hanseniano na adesão ao tratamento medicamentoso, de modo a conhecer as atividades de vida destes pacientes para possível adequação de ações voltadas a essa clientela.

  13. Directly Imaging Planets with SCExAO: First Results

    Science.gov (United States)

    Currie, Thayne M.; Guyon, Olivier; Jovanovic, Nemanja; Lozi, Julien; Tamura, Motohide; Kudo, Tomoyuki; Uyama, Taichi; Garcia, Eugenio

    2017-01-01

    We present the first science results from the newly commissioned Subaru Coronagraphic Extreme Adaptive Optics project, an experimental system dedicated to image faint jovian planets around nearby stars. SCExAO is now achieving true extreme AO capability. We describe the typical performance of SCExAO, the first images of benchmark exoplanets and planet-forming disks, and SCExAO’s first science results. Finally, we briefly chart the path forward for SCExAO to achieve its full scientific capability, including imaging mature planets in reflected light.

  14. Medidas socioeducativas: cartas ao reinado do saber

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    Gislei Domingas Romanzini Lazzarotto

    2014-09-01

    Full Text Available O estudo problematiza a experiência desenvolvida na defensoria interdisciplinar de adolescentes em conflito com a lei num programa de extensão acadêmica da Univewrsidade Federal do Rio Grande do Sul, no contexto de políticas públicas brasileiras. A metodologia do estudo consiste em analisar as narrativas sobre o trabalho da equipe de defensoria interdisciplinar discutindo as experiências ali vivenciadas com um olhar crítico sobre as práticas desenvolvidas no atendimento de adolescentes que cumprem medida socioeducativa por terem cometido ato infracional. A análise conceitual é orientada pelo diálogo com o filósofo Michel Foucault, especial em seu texto " La vie des hommes infâmes”, mapeando as relações de poder e saber que regulam as práticas institucionais e visam ao controle social dos indivíduos. A análise possibilita uma reflexão sobre as ligações que são estabelecidas entre os saberes do direito, da psicologia e da pedagogia, produzindo práticas que tendem a regular e prescrever a vida de adolescentes no contexto de atendimento socioeducativo, numa perspectiva de judicialização da vida contemporânea.

  15. Toxicologia do tolueno: aspectos relacionados ao abuso

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    Letícia M.K. Forster

    1994-04-01

    Full Text Available O tolueno está presente em muitos produtos de uso doméstico e industrial e é o principal solvente envolvido no abuso de substâncias e na exposição ocupacional. O problema mais grave no estudo de patologias relacionadas ao tolueno é que este está geralmente associado, em suas preparações comerciais, a outras substâncias. O potencial tóxico do tolueno foi abordado nos seguintes aspectos: parâmetros farmacológicos; características físico-químicas; exposição; estudos clínicos; diagnóstico; pesquisa experimental; tolerância e dependência; efeitos agudos e crônicos; neurotoxicidade; teratogenicidade; doenças psiquiátricas; carcinogenicidade e tratamento. Conclui-se ser de grande importância e urgência que se realizem estudos clínicos com amostras maiores para definição mais precisa das conseqüências do uso crônico

  16. Toxicologia do tolueno: aspectos relacionados ao abuso

    Directory of Open Access Journals (Sweden)

    Forster Letícia M.K.

    1994-01-01

    Full Text Available O tolueno está presente em muitos produtos de uso doméstico e industrial e é o principal solvente envolvido no abuso de substâncias e na exposição ocupacional. O problema mais grave no estudo de patologias relacionadas ao tolueno é que este está geralmente associado, em suas preparações comerciais, a outras substâncias. O potencial tóxico do tolueno foi abordado nos seguintes aspectos: parâmetros farmacológicos; características físico-químicas; exposição; estudos clínicos; diagnóstico; pesquisa experimental; tolerância e dependência; efeitos agudos e crônicos; neurotoxicidade; teratogenicidade; doenças psiquiátricas; carcinogenicidade e tratamento. Conclui-se ser de grande importância e urgência que se realizem estudos clínicos com amostras maiores para definição mais precisa das conseqüências do uso crônico

  17. Electromagnetic DM technology meets future AO demands

    Science.gov (United States)

    Hamelinck, Roger; Rosielle, Nick; Steinbuch, Maarten; Doelman, Niek

    New deformable mirror technology is developed by the Technische Universiteit Eindhoven, Delft University of Technology and TNO Science and Industry. Several prototype adaptive deformable mirrors are realized mirrors, up to 427 actuators and ∅150mm diameter, with characteristics suitable for future AO systems. The prototypes consist of a 100µm thick, continuous facesheet on which low voltage, electromagnetic, push-pull actuators impose out-of-plane displacements. The variable reluctance actuators with ±10µm stroke and nanometer resolution are located in a standard actuator module. Each module with 61 actuators connects to a single PCB with dedicated, 16 bit, PWM based, drivers. A LVDS multi-drop cable connects up to 32 actuator modules. With the actuator module, accompanying PCB and multi-drop system the deformable mirror technology is made modular in its mechanics and electronics. An Ethernet-LVDS bridge enables any commercial PC to control the mirror using the UDP standard. Latest results of the deformable mirror technology development are presented.

  18. Adesao ao guia alimentar para populacao brasileira

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    Eliseu Verly Junior

    2013-12-01

    Full Text Available OBJETIVO : Analisar a adesão ao Guia Alimentar para População Brasileira. MÉTODOS : Amostra composta por participantes do Inquérito de Saúde de São Paulo (n = 1.661 que preencheram dois recordatórios de 24 horas. Foi utilizado modelo bivariado de efeito misto para a razão entre o consumo de energia do grupo de alimentos e o consumo calórico total. A razão estimada foi utilizada para calcular o percentual de indivíduos com consumo abaixo ou acima da recomendação. RESULTADOS : Pelo menos 80,0% da população consome abaixo do recomendado para: leite e derivados; frutas e sucos de frutas; e cereais, tubérculos e raízes; aproximadamente 60,0% para legumes e verduras; 30,0% para feijões; e 8,0% para carnes e ovos. Adolescentes apresentaram a maior inadequação para legumes e verduras (90,0%, e o estrato de maior renda foi associado à menor inadequação para óleos, gorduras e sementes oleaginosas (57,0%. CONCLUSÕES : Foi observado consumo inadequado dos grupos de alimentos relacionados com aumento do risco de doenças crônicas.

  19. Trypanosomiasis americana en el Perú: IV. Ingreso del Triatoma infestans al territorio peruano, su dispersión en éste y posibilidades de ser erradicado

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    Arístides Herrer

    1955-12-01

    Full Text Available Después de revisar diversos aspectos concernientes a la introducción del Triatoma infestans al Perú, posiblemente alrededor de 1880, y su ulterior diseminación en la región sudoccidental, se plantea la posibilidad de su erradicación. Para esto se toma en cuenta principalmente la especial topografía de la región infestada, así como los hábitos del insecto y la forma en que se dispersa a través de los valIes de la región sudoccidental del Perú.

  20. Brachyuran Crabs collected at Curaçao

    NARCIS (Netherlands)

    Rathbun, Mary J.

    1924-01-01

    In 1920 a report ¹) on Dr. BOEKE’S collection of crabs and shrimps from Curaçao and other Dutch West Indian Islands was published. Dr. VAN DER HORST’S collection forms an important addition to the fauna of Curaçao, especially as to the Xanthids and Majids, or spider crabs. Four new species were disc

  1. The Birds of Aruba, Curaçao, and Bonaire

    NARCIS (Netherlands)

    Voous, K.H.

    1957-01-01

    This is the second and concluding part of a report on the birds of the Netherlands Antilles, the first part dealt with the birds of St. Martin, Saba, and St. Eustatius (Studies fauna Curaçao Car. Is. 6, no. 25, 1955, p. 1-82). The present part will give a full account of the birds of Aruba, Curaçao,

  2. POTENCIALIDADES E ESPAÇOS AO EMPREENDEDORISMO

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    Adriano Lago

    2008-12-01

    Full Text Available A criação de negócios é apontada como uma das causas da prosperidade de um local, região, estado ou de um país. É neste sentido que o empreendedorismo tem recebido destaque nos últimos anos, como promotor de novos negócios. Captar, descrever e analisar o fenômeno do empreendedorismo é, portanto, necessário para o desenho de ações de promoção do desenvolvimento. Assim, o presente estudo aborda o empreendedorismo na piscicultura, tomando como base três casos analisados: uma propriedade com rizipiscicultura, uma com piscicultura de ciclo completo inclusive com frigorífico, e outra onde a piscicultura desenvolve-se através de um pesque-pague, com vistas a proporcionar o turismo rural. Para fins de pesquisa, entrevistaram-se os proprietários de cada estabelecimento na busca dos dados necessários para a construção de uma abordagem consistente da temática proposta, ou seja, identificar a presença do empreendedorismo e suas características particulares em cada propriedade analisada. Verificou-se que determinadas características empreendedoras são comuns aos três casos analisados: habilidade para identificar oportunidades; busca de conhecimento de sua área de atuação; senso de organização; disposição para tomar decisões; capacidade de liderança; talento para empreender; otimismo e tino empresarial; ser estrategista, além do dinamismo. Portanto, foi possível observar que o perfil empreendedor dos proprietários de cada negócio tornou possível a transformação de idéias e problemas em oportunidades reais de negócio.

  3. WIYN active optics: a platform for AO

    Science.gov (United States)

    Code, Arthur D.; Claver, Charles F.; Goble, Larry W.; Jacoby, George H.; Sawyer, David G.

    1998-09-01

    The WIYN 3.5 meter telescope is situated on the southwest ridge of Kitt Peak yielding excellent atmosphere seeing conditions. As such, the telescope and enclosure design was directed towards exploiting this feature. The primary mirror was spun cast and figured by the Steward Observatory Mirror Laboratory and the secondary mirror by Contraves. In both cases the performance exceeded the design specifications. The borosilicate primary is actively temperature controlled to within 0.2 C of the desired temperature, typically 0.5 degrees C below the ambient air. The telescope structure is also temperature controlled and the enclosure is opened to the outside ion all sides, which all heat sources are vented to ducts carrying air downwind of the facility. The primary mirror is actively controlled for low order aberrations by 66 axial actuators which are adjusted open loop via force matrix look-up tables and closed loop via real-time wavefront curvature sensing measurements. The active optics also included real-time collimation and focus control. The telescope drive and guider are capable of providing tracking to a few hundredths of a second of arc. By employing active telescope control at this level, it is possible to maintain telescope and local wavefront distortion to a level where atmospheric effects dominate the image quality. Since a significant fraction of the power in the atmospheric disturbances is contained in image motion the first step in adaptive optics control will be simple tip tilt. Studies of higher order AO system are being carried out, as well as additional test characterizing the telescope and site. It is intended to continue such studies in an attempt to establish long term variances.

  4. Août/Magnétique

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    Julia Teles

    2015-12-01

    Full Text Available Julia Teles é técnica em áudio pelo IAV e bacharela em Composição Eletroacústica pela UNESP, tendo concluído o curso em 2012. Na universidade, foi aluna de Flo Menezes e Alexandre Lunsqui, além de ter contato com diversos compositores visitantes, como Annette Vande-Gorne, Barry Truax, entre outros. Em 2011 passou a integrar o grupo NME, atuando na área de produção e composição de obras para concertos, tendo estreado as obras Espasmo, Bezimiani, Miração; chá de coca, Transposição: dois pontos e Começo-fim em concertos do grupo em diversos espaços culturais do estado. Em 2009 iniciou seu estudo do theremin, instrumento com o qual realiza trilhas ao vivo, performances, improvisações, entre outras coisas. Em 2014 cursou composição acusmática sobre suporte analógico em um curso intensivo ministrado por Annette Vande-Gorne no Musiques et Recherches, na Bélgica. Atualmente faz parte do coletivo teatral 28 Patas Furiosas, situado em São Paulo, tendo criado a trilha sonora e operado o som do primeiro espetáculo do grupo, lenz, um outro. Há cerca de três anos atua como editora de som autônoma, tendo trabalhado principalmente no cinema (14 longas-metragens, realizando foleys e edição de som como assistente do sound designer Edson Secco. (Foto/Crédito: Beatriz Moura.

  5. Sensibilidade de estirpes de Bradyrhizobium ao glyphosate

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    Rodrigo Josemar Seminoti Jacques

    2010-02-01

    Full Text Available A aplicação do glyphosate sobre a soja resistente a este herbicida pode causar prejuízos à simbiose com o rizóbio. O objetivo deste trabalho foi avaliar a sensibilidade ao herbicida glyphosate de três estirpes de Bradyrhizobium recomendadas para a produção de inoculantes de sementes de soja no Brasil. Avaliou-se o efeito das concentrações de 0,0; 5,4; 10,8; 21,6 e 43,2 µg L-1 do ingrediente ativo do glyphosate [N-(fosfonometil glicina] no meio YM líquido sobre o crescimento de B. japonicum (estirpe SEMIA 5079 e de B. elkanii (estirpe SEMIA 5019 e estirpe SEMIA 587, por meio de leituras das densidades óticas e geração de curvas de crescimento. As reduções de crescimento na presença da menor concentração do glyphosate foram de 18% para SEMIA 5079, 29% para SEMIA 5019 e de 35% para SEMIA 587, sendo, de modo geral, quanto maior a concentração do herbicida no meio de cultura maior a inibição do crescimen­to. As estirpes apresentaram sensibilidade diferencial somente às concentrações mais baixas do glyphosate; nesse caso, foi possível determinar a seguinte ordem de sensibilidade: SEMIA 587 > SEMIA 5019 > SEMIA 5079. Essa sensibilidade diferencial é dependente da concentração do herbicida, pois na presença de 43,2 µg L-1 todas as estirpes tiveram seu crescimento severamente reduzido, não havendo diferença entre elas.

  6. Robo-AO: Performance and Characterization at Palomar Observatory

    Science.gov (United States)

    Tendulkar, Shriharsh P.; Baranec, C.; Riddle, R. L.; Ramaprakash, A. N.; Law, N. M.; Kulkarni, S. R.; Dekany, R.; Bui, K.; Davis, J.; Burse, M.; Das, H.; Punnadi, S.; Chordia, P.

    2013-01-01

    Hosted at the Palomar 60-inch telescope, Robo-AO is the world's first completely autonomous, laser-beacon supported adaptive optics (AO) system, delivering diffraction-limited images in the visible and IR wavelengths. With simultaneous turbulence monitoring using a MASS-DIMM instrument, we have characterized the performance of Robo-AO as a function of local seeing, turbulence profile, laser return power and the brightness of the tip-tilt star. We shall present the various AO metrics: The full-width at half maxima of the point spread function, the Strehl ratio, the isoplanatic angle and their variations with the atmospheric and operating conditions. Strategies for optimizing robotic AO observations based on varying conditions will be discussed.

  7. The path to visible extreme adaptive optics with MagAO-2K and MagAO-X

    Science.gov (United States)

    Males, Jared R.; Close, Laird M.; Guyon, Olivier; Morzinski, Katie M.; Hinz, Philip; Esposito, Simone; Pinna, Enrico; Xompero, Marco; Briguglio, Runa; Riccardi, Armando; Puglisi, Alfio; Mazin, Ben; Ireland, Michael J.; Weinberger, Alycia; Conrad, Al; Kenworthy, Matthew; Snik, Frans; Otten, Gilles; Jovanovic, Nemanja; Lozi, Julien

    2016-07-01

    The next generation of extremely large telescopes (ELTs) have the potential to image habitable rocky planets, if suitably optimized. This will require the development of fast high order "extreme" adaptive optics systems for the ELTs. Located near the excellent site of the future GMT, the Magellan AO system (MagAO) is an ideal on-sky testbed for high contrast imaging development. Here we discuss planned upgrades to MagAO. These include improvements in WFS sampling (enabling correction of more modes) and an increase in speed to 2000 Hz, as well as an H2RG detector upgrade for the Clio infrared camera. This NSF funded project, MagAO-2K, is planned to be on-sky in November 2016 and will significantly improve the performance of MagAO at short wavelengths. Finally, we describe MagAO-X, a visible-wavelength extreme-AO "afterburner" system under development. MagAO-X will deliver Strehl ratios of over 80% in the optical and is optimized for visible light coronagraphy.

  8. Triatoma infestans colonizando em domicílios da Baixada Fluminense, Estado do Rio de Janeiro, Brasil

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    Mário B. Aragão

    1971-06-01

    Full Text Available Nos municípios de Duque de Caxias e Nova Iguaçu, que fazem parte do Grande Rio de Janeiro, foram encontrados domicílios infestados pelo T. infestans. Em duas locdades do Município de Duque de Caxias (Amapá e Piranema foram encontrados T. infestans infectados por flagelados morfologicamente indistinguíveis do Trypanosoma cruzi, sendo de notar que alguns dêsses insetos foram coletados em uma casa em que os moradores nunca tinham estado em zona afetada pela doença de Chagas. Os autores concluam que a infestação deve ser recente e discutem o problema dêsse triatomíneo estar proliferando num clima opôsto ao de sua área de dispersão e numa região onde os triatomíneos silvestres, que penetram nos domicílios, não conseguem formar colônias.

  9. Espermiogênese como ferramenta citotaxonômica para diferenciar Triatoma guazu e T. williami, espécies vetoras da doença de Chagas

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    Yago Visinho Reis

    2015-10-01

    Full Text Available Os triatomíneos pertencem à ordem Hemiptera, subordem Heteroptera, família Reduviidae e subfamília Triatominae. Todas as 148 espécies são hematófagas estritas e potenciais vetoras do protozoário Trypanosoma cruzi, agente etiológico da doença de Chagas. Algumas espécies são extremamente semelhantes do ponto de vista morfológico, o que pode dificultar o trabalho dos programas de controle de vetores. Triatoma guazu e T. williami são consideradas como espécies irmãs e não podem ser diferenciadas por diferentes abordagens, como análises morfométricas, isoenzimáticas e cromossômicas. Assim, o presente trabalho analisou as células haplóides durante a espermiogênese, com o objetivo de auxiliar na diferenciação desses vetores. A análise das espermátides de T. guazu e T. williami permitiu diferenciá-los, pois T. williami apresentou apenas um filamento heteropicnótico nas espermátides iniciais que foi parcialmente mantido durante o alongamento celular e T. guazu apresentou dois filamentos heteropicnóticos periféricos que se uniram durante o alongamento celular, dando origem a uma espermátide alongada totalmente heteropicnótica. Assim, ressaltando a importância dessa ferramenta na diferenciação de espécies relacionadas. No entanto, sugerimos que cruzamentos híbridos experimentais devem ser realizados entre esses triatomíneos, com o intuito de confirmar o status específico desses insetos vetores da doença de Chagas.Palavras-chave: Citogenética. Espermátides. Triatominae. Subcomplexo Matogrossensis. ABSTRACT Triatomines belong to the Hemiptera order, Heteroptera suborder, Reduviidae family and Triatominae subfamily. All the 148 species are haematophagous strict and potential vectors of the protozoan Trypanosoma cruzi, etiologic agent of Chagas disease. Some species are very similar from a point of view morphological, what can difficult the work of vector control programs. Triatoma guazu and T. williami are

  10. Impact of community-based vector control on house infestation and Trypanosoma cruzi infection in Triatoma infestans, dogs and cats in the Argentine Chaco.

    Science.gov (United States)

    Cardinal, M V; Lauricella, M A; Marcet, P L; Orozco, M M; Kitron, U; Gürtler, R E

    2007-09-01

    The relative impact of two community-based vector control strategies on house infestation by Triatoma infestans and Trypanosoma cruzi infection in bugs, domestic dogs and cats was assessed in two neighboring rural areas comprising 40 small villages and 323 houses in one of the regions most endemic for Chagas disease in northern Argentina. The prevalence and abundance of domestic infestation were 1.5- and 6.5-fold higher, respectively, in the area under pulsed, non-supervised control actions operating under the guidelines of the National Vector Control Program (NCVP) than in the area under sustained, supervised surveillance carried out jointly by the UBA research team and NCVP. The prevalence of infestation and infection varied widely among village groups within each area. In the pulsed control area, the prevalence of infection in bugs, dogs and cats was two- to three-fold higher than in the area under sustained surveillance, most of the infected animals qualified as autochthonous cases, and evidence of recent transmission was observed. Infection was highly aggregated at the household level and fell close to the 80/20 rule. Using multiple logistic regression analysis clustered by household, infection in dogs was associated positively and significantly with variables reflecting local exposure to infected T. infestans, thus demonstrating weak performance of the vector surveillance system. For high-risk areas in the Gran Chaco region, interruption of vector-mediated domestic transmission of T. cruzi requires residual insecticide spraying that is more intense, of a higher quality and sustained in time, combined with community participation and environmental management measures.

  11. Evidence of selection on phenotypic plasticity and cost of plasticity in response to host-feeding sources in the major Chagas disease vector Triatoma infestans.

    Science.gov (United States)

    Nattero, Julieta; Leonhard, Gustavo; Gürtler, Ricardo E; Crocco, Liliana B

    2015-12-01

    Phenotypic plasticity is the ability of a genotype to display alternative phenotypes in different environments. Understanding how plasticity evolves and the factors that favor and constrain its evolution have attracted great interest. We investigated whether selection on phenotypic plasticity and costs of plasticity affect head and wing morphology in response to host-feeding sources in the major Chagas disease vector Triatoma infestans. Full-sib families were assigned to blood-feeding on either live pigeons or guinea pigs throughout their lives. We measured diet-induced phenotypic plasticity on wing and head size and shape; characterized selection on phenotypic plasticity for female and male fecundity rates, and evaluated costs of plasticity. Wing size and shape variables exhibited significant differences in phenotypic plasticity associated with host-feeding source in female and male bugs. Evidence of selection on phenotypic plasticity was detected in head size and shape for guinea pig-fed females. A lower female fecundity rate was detected in more plastic families for traits that showed selection on plasticity. These results provide insights into the morphological phenotypic plasticity of T. infestans, documenting fitness advantages of head size and shape for females fed on guinea pigs. This vector species showed measurable benefits of responding plastically to environmental variation rather than adopting a fixed development plan. The presence of cost of plasticity suggests constraints on the evolution of plasticity. Our study indicates that females fed on guinea pigs (and perhaps on other suitable mammalian hosts) have greater chances of evolving under selection on phenotypic plasticity subject to some constraints.

  12. Biological Control of the Chagas Disease Vector Triatoma infestans with the Entomopathogenic Fungus Beauveria bassiana Combined with an Aggregation Cue: Field, Laboratory and Mathematical Modeling Assessment.

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    Lucas Forlani

    2015-05-01

    Full Text Available Current Chagas disease vector control strategies, based on chemical insecticide spraying, are growingly threatened by the emergence of pyrethroid-resistant Triatoma infestans populations in the Gran Chaco region of South America.We have already shown that the entomopathogenic fungus Beauveria bassiana has the ability to breach the insect cuticle and is effective both against pyrethroid-susceptible and pyrethroid-resistant T. infestans, in laboratory as well as field assays. It is also known that T. infestans cuticle lipids play a major role as contact aggregation pheromones. We estimated the effectiveness of pheromone-based infection boxes containing B. bassiana spores to kill indoor bugs, and its effect on the vector population dynamics. Laboratory assays were performed to estimate the effect of fungal infection on female reproductive parameters. The effect of insect exuviae as an aggregation signal in the performance of the infection boxes was estimated both in the laboratory and in the field. We developed a stage-specific matrix model of T. infestans to describe the fungal infection effects on insect population dynamics, and to analyze the performance of the biopesticide device in vector biological control.The pheromone-containing infective box is a promising new tool against indoor populations of this Chagas disease vector, with the number of boxes per house being the main driver of the reduction of the total domestic bug population. This ecologically safe approach is the first proven alternative to chemical insecticides in the control of T. infestans. The advantageous reduction in vector population by delayed-action fungal biopesticides in a contained environment is here shown supported by mathematical modeling.

  13. Hunting, swimming, and worshiping: human cultural practices illuminate the blood meal sources of cave dwelling Chagas vectors (Triatoma dimidiata in Guatemala and Belize.

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    Lori Stevens

    2014-09-01

    Full Text Available Triatoma dimidiata, currently the major Central American vector of Trypanosoma cruzi, the parasite that causes Chagas disease, inhabits caves throughout the region. This research investigates the possibility that cave dwelling T. dimidiata might transmit the parasite to humans and links the blood meal sources of cave vectors to cultural practices that differ among locations.We determined the blood meal sources of twenty-four T. dimidiata collected from two locations in Guatemala and one in Belize where human interactions with the caves differ. Blood meal sources were determined by cloning and sequencing PCR products amplified from DNA extracted from the vector abdomen using primers specific for the vertebrate 12S mitochondrial gene. The blood meal sources were inferred by ≥ 99% identity with published sequences. We found 70% of cave-collected T. dimidiata positive for human DNA. The vectors had fed on 10 additional vertebrates with a variety of relationships to humans, including companion animal (dog, food animals (pig, sheep/goat, wild animals (duck, two bat, two opossum species and commensal animals (mouse, rat. Vectors from all locations fed on humans and commensal animals. The blood meal sources differ among locations, as well as the likelihood of feeding on dog and food animals. Vectors from one location were tested for T. cruzi infection, and 30% (3/10 tested positive, including two positive for human blood meals.Cave dwelling Chagas disease vectors feed on humans and commensal animals as well as dog, food animals and wild animals. Blood meal sources were related to human uses of the caves. We caution that just as T. dimidiata in caves may pose an epidemiological risk, there may be other situations where risk is thought to be minimal, but is not.

  14. High levels of Trypanosoma cruzi DNA determined by qPCR and infectiousness to Triatoma infestans support dogs and cats are major sources of parasites for domestic transmission.

    Science.gov (United States)

    Enriquez, G F; Bua, J; Orozco, M M; Wirth, S; Schijman, A G; Gürtler, R E; Cardinal, M V

    2014-07-01

    The competence of reservoir hosts of vector-borne pathogens is directly linked to its capacity to infect the vector. Domestic dogs and cats are major domestic reservoir hosts of Trypanosoma cruzi, and exhibit a much higher infectiousness to triatomines than seropositive humans. We quantified the concentration of T. cruzi DNA in the peripheral blood of naturally-infected dogs and cats (a surrogate of intensity of parasitemia), and evaluated its association with infectiousness to the vector in a high-risk area of the Argentinean Chaco. To measure infectiousness, 44 infected dogs and 15 infected cats were each exposed to xenodiagnosis with 10-20 uninfected, laboratory-reared Triatoma infestans that blood-fed to repletion and were later individually examined for infection by optical microscopy. Parasite DNA concentration (expressed as equivalent amounts of parasite DNA per mL, Pe/mL) was estimated by real-time PCR amplification of the nuclear satellite DNA. Infectiousness increased steeply with parasite DNA concentration both in dogs and cats. Neither the median parasite load nor the mean infectiousness differed significantly between dogs (8.1Pe/mL and 48%) and cats (9.7Pe/mL and 44%), respectively. The infectiousness of dogs was positively and significantly associated with parasite load and an index of the host's body condition, but not with dog's age, parasite discrete typing unit and exposure to infected bugs in a random-effects multiple logistic regression model. Real-time PCR was more sensitive and less time-consuming than xenodiagnosis, and in conjunction with the body condition index, may be used to identify highly infectious hosts and implement novel control strategies.

  15. Biological Control of the Chagas Disease Vector Triatoma infestans with the Entomopathogenic Fungus Beauveria bassiana Combined with an Aggregation Cue: Field, Laboratory and Mathematical Modeling Assessment

    Science.gov (United States)

    Forlani, Lucas; Pedrini, Nicolás; Girotti, Juan R.; Mijailovsky, Sergio J.; Cardozo, Rubén M.; Gentile, Alberto G.; Hernández-Suárez, Carlos M.; Rabinovich, Jorge E.; Juárez, M. Patricia

    2015-01-01

    Background Current Chagas disease vector control strategies, based on chemical insecticide spraying, are growingly threatened by the emergence of pyrethroid-resistant Triatoma infestans populations in the Gran Chaco region of South America. Methodology and findings We have already shown that the entomopathogenic fungus Beauveria bassiana has the ability to breach the insect cuticle and is effective both against pyrethroid-susceptible and pyrethroid-resistant T. infestans, in laboratory as well as field assays. It is also known that T. infestans cuticle lipids play a major role as contact aggregation pheromones. We estimated the effectiveness of pheromone-based infection boxes containing B. bassiana spores to kill indoor bugs, and its effect on the vector population dynamics. Laboratory assays were performed to estimate the effect of fungal infection on female reproductive parameters. The effect of insect exuviae as an aggregation signal in the performance of the infection boxes was estimated both in the laboratory and in the field. We developed a stage-specific matrix model of T. infestans to describe the fungal infection effects on insect population dynamics, and to analyze the performance of the biopesticide device in vector biological control. Conclusions The pheromone-containing infective box is a promising new tool against indoor populations of this Chagas disease vector, with the number of boxes per house being the main driver of the reduction of the total domestic bug population. This ecologically safe approach is the first proven alternative to chemical insecticides in the control of T. infestans. The advantageous reduction in vector population by delayed-action fungal biopesticides in a contained environment is here shown supported by mathematical modeling. PMID:25969989

  16. Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans: Expression analysis in deltamethrin susceptible and resistant populations.

    Science.gov (United States)

    Grosso, Carla G; Blariza, María J; Mougabure-Cueto, Gastón; Picollo, María I; García, Beatriz A

    2016-10-01

    Cytochrome P450 monooxygenases play a predominant role in the metabolism of insecticides. Many insect P450 genes have frequently been associated with detoxification processes allowing the insect to become tolerant or resistant to insecticides. The increases of expression of P450 genes at transcriptional level are often consider responsible for increasing the metabolism of insecticides and seems to be a common phenomenon in the evolution of resistance development in insects. As pyrethroid resistance has been detected in Triatoma infestans, it was of interest to analyze genes associated with resistance to insecticides such as those encoding for cytochromes P450. With this purpose, the cDNA sequences of three cytochrome P450 genes (CYP4EM7, CYP3085B1, and CYP3092A6) were identified in this species. Primers and specific Taqman probes were designed from these sequences to determine their expression by quantitative PCR. The mRNA levels of the cytochrome P450 genes identified were determined from total RNA extracted from pools of fat body collected from individuals of different resistant and susceptible strains of T. infestans, and at different interval times after the topical application of the lethal doses 50% (LD50) of deltamethrin on the ventral abdomen of insects belonging to the different populations analyzed. It was detected overexpression of the CYP4EM7 gene in the most resistant strain of T. infestans and the expression of the three cytochrome P450 genes isolated was induced by deltamethrin in the susceptible and resistant populations included in this study. These results suggest that these genes would be involved in the detoxification of deltamethrin and support the hypothesis that considers to the cytochrome P450 genes of importance in the development of pyrethroid resistance.

  17. Chemical communication in Chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults.

    Science.gov (United States)

    Manrique, Gabriel; Vitta, Ana C R; Ferreira, Raquel A; Zani, Carlos L; Unelius, C Rikard; Lazzari, Claudio R; Diotaiuti, Lileia; Lorenzo, Marcelo G

    2006-09-01

    Compounds from the metasternal and Brindley's glands of the blood-sucking bug, Triatoma infestans, were identified by solid phase microextraction (SPME) and gas chromatography-mass spectrometry. Volatile compounds released by adult bugs during copulation or after mechanical disturbance were also characterized. Six compounds were identified and found consistently in all samples from metasternal glands. The most abundant were 3-pentanone, 2-methylbutanol, 3-pentanol, and an unidentified compound. The metasternal gland blends did not differ qualitatively between sexes. Compounds found in Brindley's glands were short chain acids, alcohols, esters, and a ketone with no qualitative differences between sexes. Isobutyric acid was the main component of this blend, and two new confirmed compounds were described as products of these glands: 2-butanone and 2-methylbutyric acid. 3-Pentanone was collected from the headspace over 33% of the copulating pairs of T. infestans. Volatiles found in the headspace of disturbed T. infestans adults included short-chain fatty acids, alcohols, esters, and ketones, with no qualitative differences between sexes. Both types of glands apparently discharge their contents after disturbance. However, most of the volatiles released by bugs after disturbance came from Brindley's glands. The locomotor activity of fourth instars increased significantly after stimulation with the odors emitted by disturbed adults, as compared with larvae stimulated by the odor of undisturbed adults or by clean air. We also studied the directional behavioral response of fifth instars to the disturbance scent in a locomotion compensator. Larvae exposed to volatiles released by disturbed adults walked away from the direction of the odor. The results suggest that this blend or part of it functions as an alarm pheromone for T. infestans. We suggest that the metasternal glands of this species are involved both in the sexual and the alarm contexts, and that the Brindley

  18. Técnica para a observação do comportamento do Triatoma infestans (Klug, 1834 em uma miniatura de casa de pau-a-pique e sapê Technic for the observation of the behavior of Triatoma infestans (Klug, 1834 in a miniature of a mud-walled and tatch roofed house

    Directory of Open Access Journals (Sweden)

    José Roberto Mac Cord

    1983-06-01

    Full Text Available Com o objetivo de observar e registrar o comportamento do Triatoma infestans quanto a movimentos, posturas e estados fisiológicos, como preconizam os etólogos, foi construída uma réplica de uma casa de paua-pique e sapê com uma proteção externa de acrílico transparente. Para o registro das atividades empregou-se a cinematografia com lapso de tempo, através de uma filmadora super-8 sincronizada a um flash eletrônico e programada para disparos simultâneos de 1 fotograma a cada 30 segundos. A análise dos dados foi feita com um projetor super-8 e um editor, que permitiu observar cada fotograma. Com um período de registros durante 6 dias ininterruptos, os resultados permitiram concluir que: a na ausência de estímulo alimentar, não ocorre atividade locomotora no T. infestans, independente de ser dia ou noite, mesmo com o inseto privado de alimentos; b em presençaa do estímulo alimentar a atividade locomotora ocorre durante as 24 horas do dia, embora em proporção significantemente maior no período de obscuridade.In order to observe and record the behaviour of Triatoma infestans in relation to movement, position and physiological state, like the ethologists commend, a mud-walled thatch roofed house within an external transparent acrilic protection was built. To record the bug activity a time-lapse cinematographic tecnique using a super-8 mm movie camera synchronized to and eletronic flash and programmed for simultaneous shots of one photograph every 30 seconds was employed. The analysis of the data was done with super-8 mm film projector and a movieola editor which allowed the observation of each photograph. After six days of continous observation by this method the following statements could be made: a In the absence of the alimentary stimulus the locomotory activity in T. investans does not occur, being day or night, even if the insect is deprived of its blood-meal, b In presence of an alimentary stimulus the locomotory activity

  19. The Robo-AO automated intelligent queue system

    Science.gov (United States)

    Riddle, Reed L.; Hogstrom, Kristina; Papadopoulos, Athanasios; Baranec, Christoph; Law, Nicholas M.

    2014-07-01

    Robo-AO is the first automated laser adaptive optics instrument. In just its second year of scientific operations, it has completed the largest adaptive optics surveys to date, each comprising thousands of targets. Robo-AO uses a fully automated queue scheduling system that selects targets based on criteria entered on a per observing program or per target basis, and includes the ability to coordinate with US Strategic Command automatically to avoid lasing space assets. This enables Robo-AO to select among thousands of targets at a time, and achieve an average observation rate of approximately 20 targets per hour.

  20. The Robo-AO automated intelligent queue system

    CERN Document Server

    Riddle, Reed L; Papadopoulos, Athanasios; Baranec, Christoph; Law, Nicholas M

    2014-01-01

    Robo-AO is the first automated laser adaptive optics instrument. In just its second year of scientific operations, it has completed the largest adaptive optics surveys to date, each comprising thousands of targets. Robo-AO uses a fully automated queue scheduling system that selects targets based on criteria entered on a per observing program or per target basis, and includes the ability to coordinate with US Strategic Command automatically to avoid lasing space assets. This enables Robo-AO to select among thousands of targets at a time, and achieve an average observation rate of approximately 20 targets per hour.

  1. DA PSICODINÂMICA DO TRABALHO AO DIREITO FUNDAMENTAL AO CONTEÚDO DO PRÓPRIO TRABALHO E AO MEIO AMBIENTE ORGANIZACIONAL SAUDÁVEL

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    Leonardo Vieira Wandelli

    2015-10-01

    Full Text Available O presente artigo procura traçar alguns elementos que pretendem articular como, a partir do discurso jurídico, podem ser significados os aportes sobre saúde e trabalho, especialmente aqueles trazidos pela abordagem da Psicodinâmica do Trabalho. Um caminho, assim, que vai dos estudos sobre sujeito, trabalho e saúde até o Direito Constitucional, que afirma como seu fundamento a dignidade humana, indissociável da centralidade antropológica do trabalho; um vínculo expressado pelo direito fundamental ao trabalho que, aqui, se propõe reconstruir a partir do desvelamento de um direito fundamental ao conteúdo do próprio trabalho, articulado ao conceito de meio ambiente organizacional do trabalho. 

  2. Acesso ao tratamento da diabetes na Bahia: por que se recorre ao judiciário?.

    OpenAIRE

    Lisboa, Erick Soares

    2015-01-01

    A judicialização da saúde é um fenômeno importante, que tem motivado a realização de inúmeros estudos no país. A Constituição do país estabelece o direito de todos e o dever do Estado em prover acesso universal as ações e serviços de saúde. Ao longo dos 25 anos do SUS houve uma ampliação do acesso a medicamentos, e a cada ano, novos fármacos vem sendo lançados no Brasil pelas indústrias farmacêuticas. A judicialização do acesso a medicamentos tem causado impactos na estruturação, no financiam...

  3. An efficient algorithm for generating AoA networks

    Directory of Open Access Journals (Sweden)

    Nasser Eddine Mouhoub

    2012-01-01

    Full Text Available The activities, in project scheduling, can be represented graphically in two different ways, by either assigning the activities to the nodes 'AoN directed acyclic graph (dag or to the arcs 'AoA dag. In this paper, a new algorithm is proposed for generating, for a given project scheduling problem, an Activity-on-Arc dag starting from the Activity-on-Node dag using the concepts of line graphs of graphs.

  4. A desigualdade espacial do Baixo Peso ao Nascer no Brasil

    Directory of Open Access Journals (Sweden)

    Marina Clarissa Barros de Melo Lima

    2013-08-01

    Full Text Available O baixo peso ao nascer (BPN é fator de risco para a morbidade, mortalidade neonatal e infantil. No Brasil os maiores percentuais de baixo peso ao nascer ocorrem em regiões de melhor situação socioeconômica. O objetivo deste artigo é conhecer a distribuição espacial de taxas de baixo peso ao nascer e a correlação com indicadores sociais e de serviço. Desenho: ecológico, tendo os estados brasileiros como unidades de análise. Utilizou-se técnica de análise espacial, dados de 2009 do SINASC, IPEA e IBGE. Taxas maiores de baixo peso ao nascer estão nos estados da região sul/sudeste, Moran Global: 0,267, p = 0,02. Aglomerados do tipo alto-alto na região Sudeste e baixo-baixo em estados da região da Amazônia Legal. A desigualdade espacial do baixo peso ao nascer reflete as condições socioeconômicas dos estados. Regiões mais desenvolvidas detêm maiores taxas de baixo peso ao nascer, portanto, a presença do serviço e sua utilização fazem diminuir a mortalidade infantil e aumentar o BPN.

  5. Visible AO Observations at Halpha for Accreting Young Planets

    Science.gov (United States)

    Close, L. M.; Follette, K.; Males, J. R.; Morzinski, K.; Rodigas, T. J.; Hinz, P.; Wu, Y.-L.; Apai, D.; Najita, J.; Puglisi, A.; Esposito, S.; Riccardi, A.; Bailey, V.; Xompero, M.; Briguglio, R.; Weinberger, A.

    2014-01-01

    We utilized the new high-order (250-378 mode) Magellan Adaptive Optics system (MagAO) to obtain very high-resolution science in the visible with MagAO's VisAO CCD camera. In the good-median seeing conditions of Magellan (0.5-0.7'') we find MagAO delivers individual short exposure images as good as 19 mas optical resolution. Due to telescope vibrations, long exposure (60s) r' (0.63μm) images are slightly coarser at FWHM = 23-29 mas (Strehl ~ 28%) with bright (R Orion Trapezium θ1 Ori A, B, and C cluster members were obtained with VisAO. In particular, the 32 mas binary θ1 Ori C 1 C 2 was easily resolved in non-interferometric images for the first time. Relative positions of the bright trapezium binary stars were measured with ~ 0.6-5 mas accuracy. In the second commissioning run we were able to correct 378 modes and achieved good contrasts (Strehl>20% on young transition disks at Hα). We discuss the contrasts achieved at Hα and the possibility of detecting low mass (~ 1-5 Mjup) planets (past 5AU) with our new SAPPHIRES survey with MagAO at Hα.

  6. Ecologia domestica de la tripanosomiasis americana: perfil alimentario del triatoma infestans en un area rural de la província de Santiago del Estero, Argentina

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    Cristina Wisnivesky-Colli

    1987-03-01

    Full Text Available Se describen los resultados entomológicos de un estúdio sobre transmiswn del Tripanosoma cruzi realizado en un caserío rural de Argentina. Se determino la prevalencia de infección elperfil alimentario de los Triatoma infestans domiciliados, relacionándose su densidad con el tipo de vivienda. Seis de las 18 viviendas estudiadas eran nuevas o mejoradas y en 16 los habitantes aplicaban hexaclorociclohexano. La menor densidad de vinchucas correspondió a las casas higiénicas o mejoradas con tratamiento químico, Los ranchos tratados presentaron un número de insectos 10 veces menor que un rancho no tratado, indicando la efectividad del control autogestado. Se hallaron T. infestans en el 100% de los dormitorios pero en sólo el 53% de los localesperidomiciliarios. Un 39% de las vinchucas intradomiciliarias estaban infectadas. El 96% de los insectos de dormitorios se alimentaron sobre hombre, perro, polloygato, siendo el índice afinidad para el perro 13 veces superior al correspondiente al hombre. Se discute la importancia epidemiológica de la concentración de vectores dentro de los dormitorios y su asociación trófica con los perros convivientes, nsí como la necessidad de combinar la mejora de vivienda con educación sanitaria.Entomological aspects of an ecological study on domestic T. cruzi transmission carried out in a rural community of Argentina are described. T. cruzi infection rates an d the feeding profiles of domiciliary T. infestans were determined, and bug density was related to house construction. Six of 18 houses studied were new and reformed while 16 households hexachlorocyclohexane (HCH had been used by the inmates. The lowest bug densities were found in new houses or those receiving good, insecticide treatment. Insecticide treated units had an a verage number of bugs 10 times lower(3.1 bugs/man-hour than thosefound in a non treated ones indicating the effectiveness of selfp romoted control. A ll bedrooms but only 53% o f

  7. Characterizing and mitigating vibrations for SCExAO

    Science.gov (United States)

    Lozi, Julien; Guyon, Olivier; Jovanovic, Nemanja; Singh, Garima; Goebel, Sean; Norris, Barnaby; Okita, Hirofumi

    2016-07-01

    The Subaru Coronagraphic Extreme Adaptive Optics (SCExAO) instrument, under development for the Subaru Telescope, has currently the fastest on-sky wavefront control loop, with a pyramid wavefront sensor running at 3.5 kHz. But even at that speed, we are still limited by low-frequency vibrations. The current main limitation was found to be vibrations attributed mainly to the rotation of the telescope. Using the fast wavefront sensors, cameras and accelerometers, we managed to identify the origin of most of the vibrations degrading our performance. Low-frequency vibrations are coming from the telescope drive in azimuth and elevation, as well as the elevation encoders when the target is at transit. Other vibrations were found at higher frequency coming from the image rotator inside Subaru's adaptive optics facility AO188. Different approaches are being implemented to take care of these issues. The PID control of the image rotator has been tuned to reduce their high-frequency contribution. We are working with the telescope team to tune the motor drives and reduce the impact of the elevation encoder. A Linear Quadratic Gaussian controller (LQG, or Kalman filter) is also being implemented inside SCExAO to control these vibrations. These solutions will not only improve significantly SCExAOs performance, but will also help all the other instruments on the Subaru Telescope, especially the ones behind AO188. Ultimately, this study will also help the development of the TMT, as these two telescopes share very similar drives.

  8. [Summary of results from the national surveys].

    Science.gov (United States)

    Passos, Afonso Dinis Costa; Silveira, Antônio Carlos

    2011-01-01

    This article aims to correlate the main results of three large national surveys on Chagas disease (entomologic, seroprevalence and electrocardiographic) carried out in Brazil from late 1970's to early 1980's, which served as baseline for definition of the control measures adopted in the country. The proportion of infected people was much higher in areas where Triatoma infestans, the most efficient vector of Chagas disease among the five principal species involved in transmission at that time, was predominant. Similar result was observed in places where Triatoma sordida was dispersed, mainly in the country's central region, which corresponds to its native area. This finding is due to the coincidence observed in the geographic distribution of both vectors, since T. sordida is not considered to play an important role in transmission. In the Northeastern semi-arid, endemic area for Triatoma brasiliensis and Triatoma pseudomaculata, rates of human infection were much lower, although both vectors may have some relevance in the maintenance of the disease. As for areas with Panstrongylus megistus, human infection varied according to the levels of domiciliation. Whenever domiciled, like in the humid northeastern coastal area, its involvement in transmission can be clearly demonstrated. In some parts of Bahia State it represented the exclusive vector of the disease. Based upon the results of the seroprevalence survey an electrocardiographic study was carried out in 11 Brazilian states, which showed marked differences in the presence of cardiac alterations when comparing different areas of the country.

  9. Second generation Robo-AO instruments and systems

    Science.gov (United States)

    Baranec, Christoph; Riddle, Reed; Law, Nicholas M.; Chun, Mark R.; Lu, Jessica R.; Connelley, Michael S.; Hall, Donald; Atkinson, Dani; Jacobson, Shane

    2014-07-01

    The prototype Robo-AO system at the Palomar Observatory 1.5-m telescope is the world's first fully automated laser adaptive optics instrument. Scientific operations commenced in June 2012 and more than 12,000 observations have since been performed at the ~0.12" visible-light diffraction limit. Two new infrared cameras providing high-speed tip-tilt sensing and a 2' field-of-view will be integrated in 2014. In addition to a Robo-AO clone for the 2-m IGO and the natural guide star variant KAPAO at the 1-m Table Mountain telescope, a second generation of facility-class Robo-AO systems are in development for the 2.2-m University of Hawai'i and 3-m IRTF telescopes which will provide higher Strehl ratios, sharper imaging, ~0.07", and correction to λ = 400 nm.

  10. Second generation Robo-AO instruments and systems

    CERN Document Server

    Baranec, Christoph; Law, Nicholas M; Chun, Mark R; Lu, Jessica R; Connelley, Michael S; Hall, Donald; Atkinson, Dani; Jacobson, Shane

    2014-01-01

    The prototype Robo-AO system at the Palomar Observatory 1.5-m telescope is the world's first fully automated laser adaptive optics instrument. Scientific operations commenced in June 2012 and more than 12,000 observations have since been performed at the ~0.12" visible-light diffraction limit. Two new infrared cameras providing high-speed tip-tilt sensing and a 2' field-of-view will be integrated in 2014. In addition to a Robo-AO clone for the 2-m IGO and the natural guide star variant KAPAO at the 1-m Table Mountain telescope, a second generation of facility-class Robo-AO systems are in development for the 2.2-m University of Hawai'i and 3-m IRTF telescopes which will provide higher Strehl ratios, sharper imaging, ~0.07", and correction to {\\lambda} = 400 nm.

  11. A esquizofrenia ao longo da infância

    Directory of Open Access Journals (Sweden)

    Claude Bursztejn

    Full Text Available As pesquisas sobre os grupos “de alto risco” (filhos de pais esquizofrênicos, assim como os estudos prospectivos na população geral, mostram que os futuros esquizofrênicos apresentam, comparativamente aos sujeitos-controle, atrasos do desenvolvimento psicomotor, déficits cognitivos e algumas particularidades comportamentais. Tais dados parecem confirmar a idéia segundo a qual a esquizofrenia corresponderia a um distúrbio neurodesenvolvimental cuja expressão varia ao longo da vida. As especificidades clínicas e evolutivas dos raros casos de esquizofrenia iniciando-se na infância levam alguns autores a pensar que se trataria de uma entidade específica e cujo pertencimento ao “espectro autista” necessita ainda ser estudado.

  12. Insects found in birds' nests from Argentina: Coryphistera alaudina Burmeister, 1860 (Aves: Furnariidae), their inquiline birds and mammals, new hosts for Psammolestes coreodes Bergroth, 1911 and Triatoma platensis Neiva, 1913 (Hemiptera: Reduviidae: Triatominae).

    Science.gov (United States)

    Turienzo, Paola; Di Iorio, Osvaldo

    2014-06-03

    The insect fauna in nests of Coryphistera alaudina Burmeister, 1860 (Aves: Furnariidae) were studied in the provinces of Santiago del Estero, Chaco, Córdoba, and La Pampa in Argentina. A total of 7364 insect specimens comprising 77 taxa in a total of 29 families and 7 orders was found in their nests: 40 identified to species, 23 identified to genus, and 14 identified to family. Coryphistera alaudina and some of their vertebrate inquilines are new host records for the triatomine bugs Psammolestes coreodes Bergroth, 1911 and/or Triatoma platensis Neiva, 1913 (Hemiptera: Reduviidae). The insects in the nests of C. alaudina are separated by functional guilds, and their permanence time inside the nests are presented in a new manner and discussed.

  13. Beyond the Blur: Construction and Characterization of the First Autonomous AO System, and, An AO Survey of Magnetar Proper Motions

    Science.gov (United States)

    Tendulkar, Shriharsh Prakash

    Adaptive optics (AO) corrects distortions created by atmospheric turbulence and delivers diffraction-limited images on ground-based telescopes. The vastly improved spatial resolution and sensitivity has been utilized for studying everything from the magnetic fields of sunspots upto the internal dynamics of high-redshift galaxies. This thesis about AO science from small and large telescopes is divided into two parts: Robo-AO and magnetar kinematics. In the first part, I discuss the construction and performance of the world's first fully autonomous visible light AO system, Robo-AO, at the Palomar 60-inch telescope. Robo-AO operates extremely efficiently with an overhead Robo-AO is used to constrain the evolutionary models of low-mass pre-main-sequence stars by measuring resolved spectral energy distributions of stellar multiples in the visible band, more than doubling the current sample. I conclude this part with a discussion of possible future improvements to the Robo-AO system. In the second part, I describe a study of magnetar kinematics using high-resolution near-infrared (NIR) AO imaging from the 10-meter Keck II telescope. Measuring the proper motions of five magnetars with a precision of upto 0.7 milli-arcsecond/yr -1, we have more than tripled the previously known sample of magnetar proper motions and proved that magnetar kinematics are equivalent to those of radio pulsars. We conclusively showed that SGR 1900+14 and SGR 1806-20 were ejected from the stellar clusters with which they were traditionally associated. The inferred kinematic ages of these two magnetars are 6 +/- 1.8 kyr and 650 +/-3 00 yr respectively. These ages are a factor of three to four times greater than their respective characteristic ages. The calculated braking index is close to unity as compared to three for the vacuum dipole model and 2.5--2.8 as measured for young pulsars. I conclude this section by describing a search for NIR counterparts of new magnetars and a future promise of

  14. Estudio seroepidemiológico y entomológico sobre la enfermedad de Chagas en un área infestada por Triatoma maculata (Erichson 1848 en el centro-occidente de Venezuela An entomological and seroepidemiological study of Chagas' disease in an area in central-western Venezuela infested with Triatoma maculata (Erichson 1848

    Directory of Open Access Journals (Sweden)

    María Elena Rojas

    2008-10-01

    Full Text Available En el presente trabajo se realizó un estudio seroepidemiológico, entomológico y de factores de riesgo para la infestación de las viviendas en un área infestada por Triatoma maculata (Parroquia Xaguas, Municipio Urdaneta, Estado Lara, Venezuela. Se muestrearon 140 viviendas, 509 personas y 110 cánidos, a los cuales se les determinó anticuerpos séricos anti-Trypanosoma cruzi mediante ELISA y MABA, utilizando antígenos recombinantes. La infección por Tr. cruzi de los triatominos fue determinada por microscopía óptica y PCR. Los resultados mostraron una seroprevalencia en humanos de 1,57% y en cánidos de 6,36%. De los 545 triatominos capturados 97,98% fueron T. maculata, 1,65% Eratyrus mucronatus y 0,37% Panstrongylus geniculatus; con índices vectoriales de infección 0,36%, infestación 16,4%, colonización 39,1%, coinfestación 8,6% y dispersión 100%. La presencia de vectores en el domicilio y peridomicilio estuvo asociada a la presencia de gallinas, desorden en el peridomicilio, caprinos, gallineros y/o distribución del domicilio. Los resultados permiten concluir que T. maculata es el vector predominante en la región, con capacidad de infestar y colonizar el domicilio y estaría involucrado en la transmisión de la enfermedad de Chagas.This article presents a study on seroepidemiological, entomologic, and risk factors for domiciliary infestation in a circumscribed area infested with Triatoma maculata in Parroquia Xaguas, Urdaneta Municipality, Lara State, Venezuela. One hundred and forty households, 509 persons, and 110 dogs were sampled. Serum anti-Trypanosoma cruzi antibodies were determined by means of ELISA and MABA techniques using recombinant antigens. Tr. cruzi infection in the triatomines was determined by direct microscopy and PCR. According to the results, 1.57% of humans and 6.36% of dogs were positive for serum anti-Tr. cruzi antibodies. Triatomine species were: 97.98% T. maculata, 1.65% Eratyrus mucronatus, and 0

  15. House improvements and community participation in the control of Triatoma dimidiata re-infestation in Jutiapa, Guatemala Melhorias habitacionais com participação comunitária no controle da re-infestação por Triatoma dimidiata em Jutiapa, Guatemala

    Directory of Open Access Journals (Sweden)

    Carlota Monroy

    2009-01-01

    Full Text Available The deterioration or absence of plaster walls in houses and poor hygienic conditions are the most important risk factors for indoor Triatoma dimidiata infestation in Guatemala. A cross-disciplinary study was conducted addressing T. dimidiata infestation, household hygiene, and housing construction. The study focused on local materials and cultural aspects (including gender roles that could lead to long-term improvements in wall construction. A new plaster mix for walls was developed on the basis of laboratory studies on construction materials recommended by local villagers. Four villages with persistent (post-spraying T. dimidiata infestation were studied. In two villages, an ecosystem approach was implemented, and the homeowners conducted wall improvements and household sanitation with the support of the interdisciplinary team (the ecosystem intervention. In the other two villages, a vector control approach based on insecticide spraying was adopted (traditional intervention. Both interventions were associated with a reduction in T. dimidiata infestation, but only the ecosystem approach produced important housing improvements (sanitation and wall construction capable of preventing T. dimidiata re-infestation in the long term.A degradação ou ausência de reboco nas paredes e as condições higiênicas deficientes são os fatores de risco mais importantes para a infestação intradomiciliar por Triatoma dimidiata na Guatemala. Realizamos um estudo transdisciplinar sobre infestação por T. dimidiata, higiene intradomiciliar e condições de construção. O estudo destacou as questões de materiais locais e aspectos culturais (inclusive papéis de gênero que poderiam levar a melhorias nas condições das paredes no longo prazo. Formulou-se uma nova mistura de gesso para paredes, após estudos de laboratório sobre materiais de construção com base em recomendações dos residentes locais. Foram estudados quatro vilarejos com infestação por T

  16. Uma leitura da Carta ao pai de Kafka

    Directory of Open Access Journals (Sweden)

    Filipe Pereirinha

    2015-02-01

    Full Text Available ResumoPode uma carta chegar ao seu destino mesmo quando não é enviada? A Carta ao pai, de Kakfa, serve-nos aqui de balão de ensaio para esboçar uma possível resposta a esta questão. Não se trata, porém, de ler esta carta como se ela traduzisse finalmente, ao pé da letra, o conflito edipiano entre Kafka e o seu pai, mas antes de ver neste suposto conflito – e seus impasses – o limiar de outra coisa bem mais real. Palavras-chave: Kafka; Lacan; letra; sintoma; realFilipe Pereirinha é membro da Antena do Campo Freudiano (ACF - Portugal, associação psicanalítica ligada à New Lacanian School (NLS e à Associação Mundial de Psicanálise (AMP. O seu encontro com a psicanálise de orientação lacaniana aconteceu durante a licenciatura em Filosofia, na Universidade Nova de Lisboa (UNL, e foi determinante no rumo posterior dos seus estudos, tanto ao nível do mestrado em Filosofia, com uma tese sobre o desejo em Deleuze e Lacan, como ao nível do doutoramento em Filosofia Moderna e Contemporânea, com uma tese sobre a questão do sujeito em Lacan. Foi Professor e Investigador na Universidade Lusófona (ULHT, onde esteve ligado, em particular, desde a sua criação, ao Centro de Estudos de Psicanálise (CEP. Tem vários artigos publicados em livros e revistas, nomeadamente em Portugal, França e Brasil. Destacam-se os livros Psicanálise & Arredores e Uma Pedra no Caminho (no prelo. Está em preparação uma coletânea com os artigos mais recentes.

  17. Reconhecimento de aprendizagens ao longo da vida e empowerment

    OpenAIRE

    Miguel, Marta Cristina Trindade

    2015-01-01

    A narrativa atual de aprendizagem ao longo da vida apela à necessidade constante de aquisição de conhecimentos e competências para fazer face às constantes mudanças da sociedade moderna, reconhecendo que essas aprendizagens vão além da educação e formação formal, incluindo a aprendizagem decorrente da experiência de vida em diferentes contextos. O reconhecimento de aprendizagens prévias é um processo de avaliação dos conhecimentos e competências adquiridos ao longo da vida, permitindo que sej...

  18. Initial Performance of the Keck AO Wavefront Controller System

    Energy Technology Data Exchange (ETDEWEB)

    Johansson, E M; Acton, D S; An, J R; Avicola, K; Beeman, B V; Brase, J M; Carrano, C J; Gathright, J; Gavel, D T; Hurd, R L; Lai, O; Lupton, W; Macintosh, B A; Max, C E; Olivier, S S; Shelton, J C; Stomski, P J; Tsubota, K; Waltjen, K E; Watson, J A; Wizinowich, P L

    2001-03-01

    The wavefront controller for the Keck Observatory AO system consists of two separate real-time control loops: a tip-tilt control loop to remove tilt from the incoming wavefront, and a deformable mirror control loop to remove higher-order aberrations. In this paper, we describe these control loops and analyze their performance using diagnostic data acquired during the integration and testing of the AO system on the telescope. Disturbance rejection curves for the controllers are calculated from the experimental data and compared to theory. The residual wavefront errors due to control loop bandwidth are also calculated from the data, and possible improvements to the controller performance are discussed.

  19. High-Performance CCSDS AOS Protocol Implementation in FPGA

    Science.gov (United States)

    Clare, Loren P.; Torgerson, Jordan L.; Pang, Jackson

    2010-01-01

    The Consultative Committee for Space Data Systems (CCSDS) Advanced Orbiting Systems (AOS) space data link protocol provides a framing layer between channel coding such as LDPC (low-density parity-check) and higher-layer link multiplexing protocols such as CCSDS Encapsulation Service, which is described in the following article. Recent advancement in RF modem technology has allowed multi-megabit transmission over space links. With this increase in data rate, the CCSDS AOS protocol implementation needs to be optimized to both reduce energy consumption and operate at a high rate.

  20. LGS AO Science Impact: Present and Future Perspectives

    OpenAIRE

    Liu, Michael C.

    2008-01-01

    The recent advent of laser guide star adaptive optics (LGS AO) on the largest ground-based telescopes has enabled a wide range of high angular resolution science, previously infeasible from ground-based and/or space-based observatories. As a result, scientific productivity with LGS has seen enormous growth in the last few years, with a factor of ~10 leap in publication rate compared to the first decade of operation. Of the 54 refereed science papers to date from LGS AO, half have been publish...

  1. A Prediction of the Damping Properties of Hindered Phenol AO-60/polyacrylate Rubber (AO-60/ACM) Composites through Molecular Dynamics Simulation

    Science.gov (United States)

    Yang, Da-Wei; Zhao, Xiu-Ying; Zhang, Geng; Li, Qiang-Guo; Wu, Si-Zhu

    2016-05-01

    Molecule dynamics (MD) simulation, a molecular-level method, was applied to predict the damping properties of AO-60/polyacrylate rubber (AO-60/ACM) composites before experimental measures were performed. MD simulation results revealed that two types of hydrogen bond, namely, type A (AO-60) -OH•••O=C- (ACM), type B (AO-60) - OH•••O=C- (AO-60) were formed. Then, the AO-60/ACM composites were fabricated and tested to verify the accuracy of the MD simulation through dynamic mechanical thermal analysis (DMTA). DMTA results showed that the introduction of AO-60 could remarkably improve the damping properties of the composites, including the increase of glass transition temperature (Tg) alongside with the loss factor (tan δ), also indicating the AO-60/ACM(98/100) had the best damping performance amongst the composites which verified by the experimental.

  2. Esporte, lazer e prevenção ao uso drogas: dos discursos equivocados aos caminhos possíveis

    OpenAIRE

    Romera, Liana Abrão

    2013-01-01

    Resumo: O presente artigo tem como objetivo questionar a maneira ingênua e simplistaque se estabelece entre prática esportiva e prevenção ao uso de drogas. É comum aconstatação de um imaginário que atribui às praticas esportivas o poder mágico de“livrar os jovens das drogas” especialmente presente nos programas sociais que têm noesporte e lazer seu instrumento de intervenção socioeducativa. Ao colocar em xequealguns desses pressupostos do senso comum, o artigo desenvolve-se a partir dadescons...

  3. Apple fruit copper amine oxidase isoforms: peroxisomal MdAO1 prefers diamines as substrates, whereas extracellular MdAO2 exclusively utilizes monoamines.

    Science.gov (United States)

    Zarei, Adel; Trobacher, Christopher P; Cooke, Alison R; Meyers, Ashley J; Hall, J Christopher; Shelp, Barry J

    2015-01-01

    4-Aminobutyrate (GABA) accumulates in apple fruit during controlled atmosphere storage. A potential source of GABA is the polyamine putrescine, which can be oxidized via copper-containing amine oxidase (CuAO), resulting in the production 4-aminobutanal/Δ(1)-pyrroline, with the consumption of O2 and release of H2O2 and ammonia. Five putative CuAO genes (MdAO genes) were cloned from apple (Malus domestica Borkh. cv. Empire) fruit, and the deduced amino acid sequences found to contain the active sites typically conserved in CuAOs. Genes encoding two of these enzymes, MdAO1 and MdAO2, were highly expressed in apple fruit and selected for further analysis. Amino acid sequence analysis predicted the presence of a C-terminal peroxisomal targeting signal 1 tripeptide in MdAO1 and an N-terminal signal peptide and N-glycosylation site in MdAO2. Transient expression of green fluorescent fusion proteins in Arabidopsis protoplasts or onion epidermal cells revealed a peroxisomal localization for MdAO1 and an extracellular localization for MdAO2. The enzymatic activities of purified recombinant MdAO1 and MdAO2 were measured continuously as H2O2 production using a coupled reaction. MdAO1 did not use monoamines or polyamines and displayed high catalytic efficiency for 1,3-diaminopropane, putrescine and cadaverine, whereas MdAO2 exclusively utilized aliphatic and aromatic monoamines, including 2-phenylethylamine and tyramine. Together, these results indicate that MdAO1 may contribute to GABA production via putrescine oxidation in the peroxisome of apple fruit under controlled atmosphere conditions. MdAO2 seems to be involved in deamination of 2-phenylethylamine, which is a step in the biosynthesis of 2-phenylethanol, a contributor to fruit flavor and flower fragrance.

  4. Doença de Chagas em Lassance, MG: Reavaliação clínico-epidemiológica 90 anos após a descoberta de Carlos Chagas Chagas' disease in Lassance, Minas Gerais State: Clinical-epidemiological re-evaluation ninety years after the discovery by Carlos Chagas

    Directory of Open Access Journals (Sweden)

    João Carlos Pinto Dias

    2002-04-01

    Full Text Available Analisa-se a trajetória da doença de Chagas em Lassance (município da descoberta de Carlos Chagas entre 1.908 a 2.001, através de registros históricos e pesquisas atuais. O município foi importante foco da tripanossomíase entre Chagas e os anos 1.980, mercê de infestação significativa das casas por Panstrongylus megistus e, mais tarde, Triatoma infestans, espécies que foram eficazmente controladas, nos últimos 20 anos. Importante no passado, a infecção chagásica é hoje residual, com uma prevalência geral de 5,03% e afetando basicamente os grupos etários elevados, não se encontrando soropositivos abaixo dos 20 anos de idade. O perfil clínico-epidemiológico dos chagásicos detectados é o habitual de áreas com transmissão interrompida, com a maioria dos casos em formas cardíacas benignas ou na forma crônica indeterminada, havendo ainda indicativos de formas digestivas, sendo a mortalidade ainda significativa, em grupos etários elevados. O município apresenta-se infestado por T. sordida, em baixas densidades e grande dispersão, não infectado por T. cruzi e restrito ao peridomicílio. Conclui-se que Lassance está hoje livre da transmissão da doença, devendo manter-se sob vigilância epidemiológica frente aos triatomíneos nativos no município e garantir-se a atenção médica às pessoas infectadas no passado.The history and present situation of Chagas' disease in Lassance (the county where Carlos Chagas discovered American trypanosomiasis were studied through a historical analysis and clinical and epidemiological research performed from 1999 to 2001. Lassance was an important focus of Chagas' disease from Carlos Chagas up until the 1980's, because of intensive infestation in dwellings by Panstrongylus megistus and Triatoma infestans, two important species which were efficiently controlled in the last twenty years. Human Chagas' disease was important in the past but today is only residual, affecting basically the

  5. On some Polychaetous Annelids from Curaçao

    NARCIS (Netherlands)

    Horst, R.

    1922-01-01

    Though we know already a rather large number of Polychaetous Annelids from the Caribbean Sea, hitherto, as far as I know, no Annelids have been described from the coast of the island Curaçao and I therefore was very glad, that my colleague Dr. VAN DER HORST kindly placed in my hands for identificati

  6. Bringing the Visible Universe into Focus with Robo-AO

    CERN Document Server

    Baranec, Christoph; Law, Nicholas M; Ramaprakash, A N; Tendulkar, Shriharsh P; Bui, Khanh; Burse, Mahesh P; Chordia, Pravin; Das, Hillol K; Davis, Jack T C; Dekany, Richard G; Kasliwal, Mansi M; Kulkarni, Shrinivas R; Morton, Timothy D; Ofek, Eran O; Punnadi, Sujit; 10.3791/50021

    2013-01-01

    Light from astronomical objects must travel through the earth's turbulent atmosphere before it can be imaged by ground-based telescopes. To enable direct imaging at maximum theoretical angular resolution, advanced techniques such as those employed by the Robo-AO adaptive-optics system must be used.

  7. Adesão ao tratamento da ambliopia

    Directory of Open Access Journals (Sweden)

    Arakaki Mara Regina

    2004-01-01

    Full Text Available OBJETIVO: Avaliar a influência da adesão no resultado do tratamento da ambliopia. MÉTODOS: Foi feito estudo retrospectivo avaliando a resposta ao tratamento da ambliopia em 151 crianças portadoras de ambliopia, tratadas com esquema de oclusão diária, com o número de dias variando de acordo com a idade da criança. Os pacientes foram separados em dois grupos que diferiam entre si pela adesão ou não ao tratamento. RESULTADOS: Observou-se que o tratamento teve melhores resultados nas crianças de 4 a 7 anos e que aderiram ao tratamento; as crianças que aderiram ao tratamento necessitaram de tempo menor de permanência em tratamento. Mesmo crianças com idade superior a 7 anos tiveram resultados positivos com a oclusão. CONCLUSÃO:O tratamento oclusivo é um meio terapêutico eficiente desde que haja a cooperação do paciente.

  8. Reptiles and Amphibians of Curaçao

    NARCIS (Netherlands)

    Rooij, de Nelly

    1922-01-01

    The Zoological Museum of Amsterdam received some collections of reptiles from Curaçao made by Dr. J. BOEKE in 1905, by Dr. C. J. VAN DER HORST in 1920 and by Mr. G. J. H. MOLENGRAAFF „Engineer” in 1921. I had the opportunity of studying them and will here give an enumeration of all the reptiles know

  9. Resposta ao estresse: II. Resiliência e vulnerabilidade

    Directory of Open Access Journals (Sweden)

    Nicole Leite Galvão-Coelho

    2015-06-01

    Full Text Available ResumoA crescente exposição a estressores na vida cotidiana aumentou significativamente a investigação da resposta ao estresse nas duas últimas décadas. Embora associada a consequências negativas, pois muitas patologias físicas e mentais são desencadeadas por exposição crônica a estressores, esta resposta é indispensável para sobrevivencia do indivíduo e é extremamente adaptativa quando ativada de forma aguda. Na parte I desta revisão foram abordados os conceitos de homestase e alostase e os sistemas fisiológicos ativados durante a resposta ao estresse. Na parte II serão discutidos fatores que modulam a resposta ao estresse tais como sexo, temperamento, períodos críticos do desenvolvimento e a presença ou ausência de suporte social. A interação entre os fatores genéticos e ambientais gera os perfis da resposta psicofisiológica que caracterizam os fenótipos de susceptibilidade e resiliência frente aos estressores e sua relação com uma patologia mental cada vez mais presente na sociedade moderna, o transtorno de estresse pós-traumático.

  10. PCR reveals significantly higher rates of Trypanosoma cruzi infection than microscopy in the Chagas vector, Triatoma infestans: High rates found in Chuquisaca, Bolivia

    Directory of Open Access Journals (Sweden)

    Lucero David E

    2007-06-01

    Full Text Available Abstract Background The Andean valleys of Bolivia are the only reported location of sylvatic Triatoma infestans, the main vector of Chagas disease in this country, and the high human prevalence of Trypanosoma cruzi infection in this region is hypothesized to result from the ability of vectors to persist in domestic, peri-domestic, and sylvatic environments. Determination of the rate of Trypanosoma infection in its triatomine vectors is an important element in programs directed at reducing human infections. Traditionally, T. cruzi has been detected in insect vectors by direct microscopic examination of extruded feces, or dissection and analysis of the entire bug. Although this technique has proven to be useful, several drawbacks related to its sensitivity especially in the case of small instars and applicability to large numbers of insects and dead specimens have motivated researchers to look for a molecular assay based on the polymerase chain reaction (PCR as an alternative for parasitic detection of T. cruzi infection in vectors. In the work presented here, we have compared a PCR assay and direct microscopic observation for diagnosis of T. cruzi infection in T. infestans collected in the field from five localities and four habitats in Chuquisaca, Bolivia. The efficacy of the methods was compared across nymphal stages, localities and habitats. Methods We examined 152 nymph and adult T. infestans collected from rural areas in the department of Chuquisaca, Bolivia. For microscopic observation, a few drops of rectal content obtained by abdominal extrusion were diluted with saline solution and compressed between a slide and a cover slip. The presence of motile parasites in 50 microscopic fields was registered using 400× magnification. For the molecular analysis, dissection of the posterior part of the abdomen of each insect followed by DNA extraction and PCR amplification was performed using the TCZ1 (5' – CGA GCT CTT GCC CAC ACG GGT GCT – 3

  11. Infestation of Palm Trees by Triatomines (Hemiptera: Reduviidae in the State of Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Rodrigo Gurgel-Gonçalves

    2012-12-01

    Resumo. As palmeiras desempenham papéis importantes como habitats de reprodução e alimentação para triatomíneos silvestres, vetores da doença de Chagas. A ocorrência de triatomíneos em palmeiras peridomiciliares pode aumentar o risco de invasão desses insetos em domicílios e deve ser considerada para desenvolver estratégias de prevenção. Com objetivo de investigar a infestação de palmeiras por triatomíneos no Estado da Bahia e determinar a infecção natural desses insetos por Trypanosoma spp., foram amostradas 183 palmeiras em 12 municípios entre 2006 e 2011 utilizando captura manual e/ou armadilhas adesivas iscadas com camundongos. Os triatomíneos foram detectados em 79 palmeiras (43% das espécies Copernicia prunifera (Mart. Becc., Mauritia flexuosa L. e Attalea spp. (Attalea burretiana Bondar ou Attalea salvadorensis Glassman. Em outras espécies de palmeiras (Syagrus coronata (Mill H.E. Moore, Attalea funifera Mart ex. Spreng e Elaeis guineensis Jacq não foram detectados triatomíneos. Rhodnius neglectus Lent, Triatoma sordida (Stål, e Triatoma pseudomaculata Corrêa & Espínola ocorreram em C. prunifera ao longo do rio São Francisco. No extremo oeste da Bahia, R. neglectus e Psammolestes tertius Lent & Jurberg foram detectados em M. flexuosa, enquanto Triatoma tibiamaculata (Pinto ocorreu em Attalea sp. em áreas urbanas de Salvador. No total, 180 triatomíneos foram capturados, principalmente R. neglectus. A maior taxa de infecção natural (61% foi observada em T. tibiamaculata. Os resultados indicam que pelo menos três espécies de palmeiras são habitats favoráveis para triatomíneos no estado da Bahia e ocorrem no ambiente peridomiciliar, o que pode aumentar a probabilidade de invasão de triatomíneos nas casas.

  12. Historicizando a teoria da atividade: do embate ao debate

    Directory of Open Access Journals (Sweden)

    Gustavo Martins Piccolo

    2012-08-01

    Full Text Available O presente artigo busca, mediante um processo de revisão de literatura, investigar analiticamente as origens, fundamentos e a estrutura da teoria da atividade, entendida como uma forma dialética de relação entre o homem e a realidade histórico-concreta, cuja materialidade é capital ao processo formativo do psiquismo humano. Tal teoria deriva-se dos estudos de Vygotsky e é sistematizada em seu aparato conceitual por Leontiev. Além disso, trazemos ao corpo do texto fundamentos acerca das discussões sobre a validade da teoria da atividade e as possíveis fontes epistemológicas e ideológicas das críticas dirigidas a tal aparato conceitual, objetivando transformar tal embate em um debate acalorado, todavia, profundamente comprometido para com uma psicologia crítica e transformadora.

  13. Sobre a viagem de Enrico Fermi ao Brasil em 1934

    CERN Document Server

    Caruso, Francisco

    2014-01-01

    Enrico Fermi was one of the greater physicists of the XX century. In 1934, he gave several lectures in Brazil. Invited by Theodoro Ramos to work in S\\~ao Paulo, he preferred to stay in Rome and went to the USA in 1938. However, Fermi recommended Gleb Wataghin to come in his place. Wataghin made history in Brazil, becoming one of the first Professors of the future S\\~ao Paulo University. Besides his relevance to the History of Science, Fermi eventually leaved an indelible mark on the creation and institutionalization of national scientific research due to the indication of Wataghin. Despite this fact, very little is known about Fermi's trip to Brazil. This work tries to reconstruct the fullest possible steps of the famous Italian physicist in our lands.

  14. Da esclerodermia ao síndrome de poems

    Directory of Open Access Journals (Sweden)

    Ana Sofia Montez

    2015-12-01

    Full Text Available Homem, 84 anos, com antecedentes de hipotiroidismo e alterações cutâneas recentes compatíveis com esclerodermia, confirmadas em biópsia cutânea, mas com estudo auto-imune negativo. Foi internado por queixas constitucionais. Ao exame objetivo, apresentava-se emagrecido, com lesões de vitiligo nas mãos além das de esclerodermia (figura 1 e distensão abdominal com ascite

  15. Da esclerodermia ao síndrome de poems

    OpenAIRE

    Ana Sofia Montez; Elsa Rocha

    2015-01-01

    Homem, 84 anos, com antecedentes de hipotiroidismo e alterações cutâneas recentes compatíveis com esclerodermia, confirmadas em biópsia cutânea, mas com estudo auto-imune negativo. Foi internado por queixas constitucionais. Ao exame objetivo, apresentava-se emagrecido, com lesões de vitiligo nas mãos além das de esclerodermia (figura 1) e distensão abdominal com ascite

  16. Cuidado ao idoso deprimido e institucionalizado à luz da Complexidade

    Directory of Open Access Journals (Sweden)

    Cenir Gonçalves Tier

    2008-06-01

    Full Text Available Instituições de Longa Permanência para Idosos (ILPI são estabelecimentos para atendimento integral, institucionalizando pessoas de 60 anos e mais, dependentes/ou não, que não dispõem de condições para permanecer com a família ou/em seu domicílio. A institucionalização pode desencadear doenças, dentre elas a depressão. Foi objetivo deste artigo de atualização fazer uma reflexão sobre a relação do cuidado ao idoso deprimido e residente em ILPI com três princípios da Complexidade segundo Edgar Morin. Primeiro, se reflete sobre a complexidade; depois, se relaciona o cuidado ao idoso deprimido e residente em ILPI, com os princípios dialógico, recursivo e hologramático. Percebeu-se que a Complexidade pode sustentar teoricamente, não só a compreensão do fenômeno depressão no idoso residente em uma ILPI, como também inspirar o modo de cuidar que direcione aos trabalhadores da saúde, que atuam junto à pessoa idosa, a abordarem o idoso de forma mais global. Para tanto deve ser utilizada a Avaliação Multidimensional do Idoso, que analisa as várias dimensões da pessoa idosa, contribuindo no desenvolvimento de um plano de cuidado/tratamento e acompanhamento multidisciplinar e interdisciplinar adequados. A Complexidade vem sendo utilizada, por enfermeiros, para entender melhor a enfermagem e prestar cuidado mais adequado ao ser humano.

  17. SUBAXIAL CERVICAL FRACTURE: APPLICATION AND CORRELATION OF AO AND SLIC

    Directory of Open Access Journals (Sweden)

    Allan Hiroshi de Araujo Ono

    2015-09-01

    Full Text Available Objective:To evaluate the AO/Magerl classification and the SLIC (Subaxial Cervical Spine Injury Classification, used in the cervical spine fractures and assess whether they are correlated to the neurological severity of patients, the choice of approach to be used, de duration of surgery an between themselves.Method:Retrospective analysis of medical records and radiological image files of 77 patients surgically treated of subaxial cervical fracture or dislocation from August 2010 to September 2012.Results:The SLIC classification showed a strong correlation with neurological deficit and Pearson correlation value of -0.600. The AO classification was not correlated with the Frankel scale and the value of Pearson was 0.06 with a statistical significance of 0.682 (p<0.05, that is, unable to determine or suggest the severity of the deficit. When compared to each other the two classifications showed statistical correlation and the value of Pearson was 0.282 with a significance value of 0.022 (p<0.05.Conclusion:Among the most used classifications, the SLIC has been able to statistically define the need for surgical treatment and the severity of the neurological status, but was unable to predict the approach or the time of the surgery; the classification AO failed to predict the severity of neurological injury, surgical time, and did not help to choose the approach, just being a morphological classification.

  18. An Advanced Atmospheric Dispersion Corrector: The Magellan Visible AO Camera

    CERN Document Server

    Kopon, Derek; Gasho, Victor

    2010-01-01

    In addition to the BLINC/MIRAC IR science instruments, the Magellan adaptive secondary AO system will have an EEV CCD47 that can be used both for visible AO science and as a wide-field acquisition camera. The effects of atmospheric dispersion on the elongation of the diffraction limited Magellan adaptive optics system point spread function (PSF) are significant in the near IR. This elongation becomes particularly egregious at visible wavelengths, culminating in a PSF that is 2000\\{mu}m long in one direction and diffraction limited (30-60 \\{mu}m) in the other over the wavelength band 0.5-1.0\\{mu}m for a source at 45\\pm zenith angle. The planned Magellan AO system consists of a deformable secondary mirror with 585 actuators. This number of actuators should be sufficient to nyquist sample the atmospheric turbulence and correct images to the diffraction limit at wavelengths as short as 0.7\\{mu}m, with useful science being possible as low as 0.5\\{mu}m. In order to achieve diffraction limited performance over this ...

  19. Dual-Frequency VSOP Observations of AO 0235+164

    CERN Document Server

    Frey, S; Altschüler, D R; Davis, M M; Perillat, P; Salter, C J; Aller, H D; Aller, M F; Hirabayashi, H

    2000-01-01

    AO 0235+164 is a very compact, flat spectrum radio source identified as a BL Lac object at a redshift of z=0.94. It is one of the most violently variable extragalactic objects at both optical and radio wavelengths. The radio structure of the source revealed by various ground-based VLBI observations is dominated by a nearly unresolved compact component at almost all available frequencies. Dual-frequency space VLBI observations of AO 0235+164 were made with the VSOP mission in January-February 1999. The array of the Japanese HALCA satellite and co-observing ground radio telescopes in Australia, Japan, China and South Africa allowed us to study AO 0235+164 with an unprecedented angular resolution at frequencies of 1.6 and 5 GHz. We report on the sub-milliarcsecond structural properties of the source. The 5-GHz observations led to an estimate of T_B > 5.8 x 10^{13} K for the rest-frame brightness temperature of the core, which is the highest value measured with VSOP to date.

  20. LDEF results for polymer matrix composite experiment AO 180

    Science.gov (United States)

    Tennyson, R. C.

    1992-01-01

    This report represents a summary of the results obtained to-date on a polymer matrix composite experiment (AO 180) located at station D-12, about 82 deg off the 'ram' direction. Different material systems comprised of graphite, boron, and aramid (Kevlar) fiber reinforcements were studied. Although previous results were presented on in-situ thermal-vacuum cycling effects, particularly dimensional changes associated with outgassing, additional comparative data will be shown from ground-based tests on control and flight samples. The system employed was fully automated for thermal-vacuum cycling using a laser interferometer for monitoring displacements. Erosion of all three classes of materials due to atomic oxygen (AO) will also be discussed, including angle of incidence effects. Data from this experiment will be compared to published results for similar materials in other LDEF experiments. Composite materials' erosion yields will be presented on an AO design nomogram useful for estimating total material loss for given exposure conditions in low Earth orbit (LEO). Optical properties of these materials will also be compared with control samples. A survey of the damage caused by micrometeoroids/debris impacts will be addressed as they relate to polymer matrix composites. Correlations between hole size and damage pattern will be given. Reference to a new nomogram for estimating the number distribution of micrometeoroid/debris impacts for a given space structure as a function of time in LEO will be addressed based on LDEF data.

  1. Um crime delicado: conversando aos infinitos - "Um retrato de cavalo"

    Directory of Open Access Journals (Sweden)

    Cid Ottoni Bylaardt

    2010-12-01

    um cavalo e seu retrato, a partir dos sentimentos contraditórios de seu dono, que considera um crime aquele clique. Temos aí um retrato, imagem subtraída ilicitamente ao seu dono: escrita, representação. Temos também uma narrativa, escrita do retrato, representação da representação. Este artigo pretende mostrar como Guimarães Rosa manipula as ambiguidades da linguagem literária, levando-a além dos limites da representação, explorando seu fascínio, seu saber, que não é da ordem da compreensão. O cavalo e seu retrato fazem refletir sobre a literatura, sobre seu excesso de verdade que compõe sua mentira. Ao escrever o cavalo e seu retrato, o texto não consegue fixar nenhuma verdade, apenas imagens fugidias que compõem recapítulos: há sempre um escrito a se sobrepor a outro, sem determinar onde está o verdadeiro, onde está o que o nega, a remeter o olhar ao reino da fascinação, onde a imagem perde o valor de significação para se tornar pura paixão da indeterminação, da indiferença.

  2. Simulations of (MC)AO for a 100-m telescope

    Science.gov (United States)

    Le Louarn, Miska; Verinaud, Christophe; Yaitskova, Natalia; Korkiakoski, Visa; Fedrigo, Enrico; Hubin, Norbert N.

    2004-10-01

    In this paper, we summarize the analysis made on various Adaptive Optics (AO) modes (including Shack-Hartmann (SH) and Pyramid Wavefront Sensors (PWS)) for the OverWhelmingly Large telescope (OWL). We will show some early results of the performance to be expected with a first generation AO system, working in the infrared. Several telescope diameters were considered to see the variations as a function of telescope diameter. This is also compatible with the concept of "grow a telescope" where the telescope diameter of OWL grows from 60m to 100m as a function of time. In a first phase, to simplify the problem, the effects of the central obstruction were neglected. However, for the Shack-Hartmann (SH) simulations, additional simulations were carried out with a simulated OWL pupil, including segmentation errors. We show some early results for a ground-layer correction system, working with three natural guide stars (NGSs) and a single deformable mirror (DM). An MCAO system based on 2 DMs, 3 NGSs is also investigated. For the last two systems, our results are found to be in very good agreement with Cibola, an analytical AO modeling tool. We show that some outer scale of turbulence effects improve slightly the correction quality when going from a telescope diameter of 10m to 100m.

  3. Resposta ao estresse: I. Homeostase e teoria da alostase

    Directory of Open Access Journals (Sweden)

    Maria Bernardete Cordeiro de Sousa

    2015-03-01

    Full Text Available ResumoOs seres vivos desenvolvem ao longo de sua história evolutiva mecanismos de enfrentamento às condições adversas originadas tanto no ambiente geofísico como no ambiente social. Esta resposta adaptativa é coordenada e envolve diferentes sistemas funcionais, particularmente, os sistemas nervoso, endócrino e imune, e é denominada de resposta ao estresse e deve atender a duas demandas principais da vida: sobrevivência e reprodução. Esta revisão tem o objetivo de discutir o emprego do conceito clássico de homeostase e um conceito alternativo, alostase, que inclui os mecanismos preditivos e reativos de regulação, assim como os diferentes níveis de impacto dos estressores crônicos, resultando em sobrecarga alostática que pode ou não se seguir de falha alostática. Os mecanismos neurais, hormonais, imunes, sistêmicos e moleculares, que compreendem os sistemas alóstaticos subjacentes à resposta ao estresse são também apresentados.

  4. Sylvatic triatomines (Hemiptera: Reduviidae) in Bolivia: trends toward domesticity and possible infection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae).

    Science.gov (United States)

    Noireau, F; Bosseno, M F; Carrasco, R; Telleria, J; Vargas, F; Camacho, C; Yaksic, N; Brenière, S F

    1995-09-01

    The risk of domestic transmission of Trypanosoma cruzi (Chagas) by sylvatic triatomines was assessed in an isolated area of the subandean region of Bolivia. None of the 390 residents examined had serological evidence of infection. Two sylvatic triatomine species, Eratyrus mucronatus (Stål) and Triatoma sordida (Stål), were found in houses and in peridomestic structures. The collection of nymphal instars of both species from some houses indicated possible domesticity. Microscopic examination of feces from 92 insects showed no parasites, and cultures from the guts of 30 insects were negative. Nevertheless, a polymerase chain reaction (PCR) test performed on the same fecal samples showed the presence of T. cruzi DNA in 19.1 and 12.5% of E. mucronatus and T. sordida, respectively. These 16 PCR-positive samples were hybridized with 2 T. cruzi-specific probes known from the domestic cycle in Bolivia (clones 20 and 39). At least 1 of these clones was identified in 7 bugs (5 E. mucronatus and 2 T. sordida). Moreover, no hybridization was observed with these probes in S E. mucronatus and 1 T. sordida samples that showed an amplified band by PCR. These data indicated that T. cruzi clones, genetically unrelated to clones 20 and 39, also were circulating in this area. Based on these results, the 2 sylvatic triatomine species encountered in Apolo should not be overlooked as possible local vectors of T. cruzi.

  5. Fatores associados a baixa adesao ao tratamento medicamentoso em idosos

    Directory of Open Access Journals (Sweden)

    Noemia Urruth Leao Tavares

    2013-12-01

    Full Text Available OBJETIVO : Analisar fatores associados à baixa adesão ao tratamento medicamentoso em idosos. MÉTODOS : Estudo transversal de base populacional, com amostra representativa de 1.593 indivíduos com 60 anos ou mais, residentes na região urbana de Bagé, RS, em 2008. A amostragem foi realizada em múltiplos estágios. Os dados foram coletados em entrevistas individuais nos domicílios. Analisou-se a associação entre a baixa adesão referida ao tratamento medicamentoso mensurado pelo Brief Medication Questionnaire (BMQ e fatores demográficos, socioeconômicos, comportamentais e de saúde, assistência e prescrição. Foi utilizado modelo de regressão de Poisson para estimar as razões de prevalência bruta e ajustada, os respectivos intervalos de confiança de 95% e p-valor (teste de Wald. RESULTADOS : Cerca de 78,0% dos indivíduos referiram ter usado algum medicamento nos sete dias precedentes à entrevista. Desses, cerca de 1 / 3 foram considerados com baixa adesão ao tratamento. Os fatores significativamente associados à baixa adesão foram: idade (65 a 74 anos, não ter plano de saúde, ter que comprar (totalmente ou em parte os seus medicamentos, ter três ou mais morbidades, possuir incapacidade instrumental para a vida diária e usar três ou mais medicamentos. CONCLUSÕES : A utilização elevada de medicamentos, decorrente da alta prevalência de doenças crônico-degenerativas em idosos, e o acesso ao tratamento devem ser considerados pelos profissionais de saúde para adoção de estratégias que visem diminuir a baixa adesão ao tratamento, aumentando a resolutividade terapêutica e a qualidade de vida desses pacientes.

  6. SIMS chemical and isotopic analysis of impact features from LDEF experiments AO187-1 and AO187-2

    Science.gov (United States)

    Stadermann, Frank J.; Amari, Sachiko; Foote, John; Swan, Pat; Walker, Robert M.; Zinner, Ernst

    1995-01-01

    Previous secondary ion mass spectrometry (SIMS) studies of extended impact features from LDEF capture cell experiment AO187-2 showed that it is possible to distinguish natural and man-made particle impacts based on the chemical composition of projectile residues. The same measurement technique has now been applied to specially prepared gold target impacts from experiment AO187-1 in order to identify the origins of projectiles that left deposits too thin to be analyzed by conventional energy-dispersive x-ray (EDX) spectroscopy. The results indicate that SIMS may be the method of choice for the analysis of impact deposits on a variety of sample surfaces. SIMS was also used to determine the isotopic compositions of impact residues from several natural projectiles. Within the precision of the measurements all analyzed residues show isotopically normal compositions.

  7. Suporte relacionado ao cuidado em saúde ao doente com aids: o modelo de comboio e a enfermagem

    Directory of Open Access Journals (Sweden)

    Edilene Aparecida Araújo da Silveira

    2011-06-01

    Full Text Available O presente estudo teve como objetivo conhecer a relação e a troca de suporte entre o doente com AIDS e pessoas à sua volta. O estudo está baseado no modelo de comboio das relações sociais que tem como uma de suas características a representação da rede em três círculos concêntricos. Os participantes eram pessoas que estavam internadas e que falaram sobre o suporte relacionado ao cuidado em saúde proporcionado por pessoas próximas, ou seja, que pertenciam ao círculo interno do comboio. A maioria dos participantes descreveu o círculo como sendo composto por no máximo cinco integrantes, sendo que pessoas da família foram as mais citadas. Os profissionais de saúde precisam conhecer o paciente e seu comboio, reconhecendo-os no contexto psicossocial e cultural de forma a favorecer a aceitação da soropositividade, mudanças no estilo de vida, ajuda nos cuidados de saúde e adesão ao tratamento.

  8. Green FLASH: energy efficient real-time control for AO

    Science.gov (United States)

    Gratadour, D.; Dipper, N.; Biasi, R.; Deneux, H.; Bernard, J.; Brule, J.; Dembet, R.; Doucet, N.; Ferreira, F.; Gendron, E.; Laine, M.; Perret, D.; Rousset, G.; Sevin, A.; Bitenc, U.; Geng, D.; Younger, E.; Andrighettoni, M.; Angerer, G.; Patauner, C.; Pescoller, D.; Porta, F.; Dufourcq, G.; Flaischer, A.; Leclere, J.-B.; Nai, A.; Palazzari, P.; Pretet, D.; Rouaud, C.

    2016-07-01

    The main goal of Green Flash is to design and build a prototype for a Real-Time Controller (RTC) targeting the European Extremely Large Telescope (E-ELT) Adaptive Optics (AO) instrumentation. The E-ELT is a 39m diameter telescope to see first light in the early 2020s. To build this critical component of the telescope operations, the astronomical community is facing technical challenges, emerging from the combination of high data transfer bandwidth, low latency and high throughput requirements, similar to the identified critical barriers on the road to Exascale. With Green Flash, we will propose technical solutions, assess these enabling technologies through prototyping and assemble a full scale demonstrator to be validated with a simulator and tested on sky. With this R&D program we aim at feeding the E-ELT AO systems preliminary design studies, led by the selected first-light instruments consortia, with technological validations supporting the designs of their RTC modules. Our strategy is based on a strong interaction between academic and industrial partners. Components specifications and system requirements are derived from the AO application. Industrial partners lead the development of enabling technologies aiming at innovative tailored solutions with potential wide application range. The academic partners provide the missing links in the ecosystem, targeting their application with mainstream solutions. This increases both the value and market opportunities of the developed products. A prototype harboring all the features is used to assess the performance. It also provides the proof of concept for a resilient modular solution to equip a large scale European scientific facility, while containing the development cost by providing opportunities for return on investment.

  9. Oficina de trabalho: "mulher-uma viagem ao seu corpo"

    Directory of Open Access Journals (Sweden)

    Maria Helena Costa Amorim

    1996-06-01

    Full Text Available Nas mulheres brasileiras, a morbidade por câncer de colo uterino e de mama é alta, mesmo que estas patologias sejam preveníveis e diagnosticadas precocemente. A cobertura dos serviços de saúde nesta área é inadequada, considerando-se a tecnologia simples e barata a ser utilizada na prevenção e detecção precoce destes tipos de câncer. Observamos que poucas são as atividades desenvolvidas com o objetivo de educar a mulher quanto ao auto conhecimento do corpo. Dessa forma, em 1993, elaboramos uma Oficina de Trabalho intitulada: " Mulher-uma viagem ao seu corpo", composta de 6 (seis dinâmicas onde a participação de cada mulher é intensa por ser um processo envolvente. A Oficina é desenvolvida por 1 (um docente, 3 (três enfermeiras e acadêmicos de Enfermagem da Universidade Federal do Espírito Santo. São inscritas 30 (trinta participantes por Oficina, sendo que cada uma recebe crachá de participação. Esta oficina é composta de 6 (cinco dinâmicas que se desenvolvem num ambiente descontraído e aconchegante: Dinâmica 1 - História de Bolso; Dinâmica 2 - Toque das Mãos; Dinâmica 3 - Desenho/Modelagem; Dinâmica 4 - Viagem ao Corpo; Dinâmica 5 - O Auto Exame; Dinâmica 6 - Percebendo o corpo.

  10. 76 FR 4726 - Avaya Global Services, AOS Service Delivery, Worldwide Services Group, Including Workers Whose...

    Science.gov (United States)

    2011-01-26

    ... Employment and Training Administration Avaya Global Services, AOS Service Delivery, Worldwide Services Group... Assistance on October 20, 2010, applicable to workers of Avaya Global Services, AOS Service Delivery... Avaya Global Services, AOS Service Delivery had their wages reported through a separate...

  11. Onicomicoses: do Diagnóstico ao Tratamento

    OpenAIRE

    Kioshima, Érika Seki; Oliveira, Ana Carla Pozzi; Svidzinski, Terezinha Inez Estivalet

    2008-01-01

    Onicomicose é um termo amplo atribuído às infecções fúngicas que atingem as unhas. Quando causada por dermatófitos, é denominada manter ungueal, e quando por leveduras, oníquias. Há ainda as paroníquias que são infecções ao redor das unhas. Os dermatófitos são fungos queratinofílicos capazes de parasitar tecidos queratinizados do homem, de animais ou restos de queratina encon...

  12. Akreos Adapt AO Intraocular Lens Opacification: A Case Report

    Directory of Open Access Journals (Sweden)

    Matteo Forlini

    2013-09-01

    Full Text Available A 79-year-old hyperglycemic patient was referred to the ‘Santa Maria delle Croci' Hospital, Ravenna, Italy. He presented with visual impairment in the right eye. Four years ago, he had had an uneventful cataract surgery in the right eye. We observed an opacification of the intraocular lens (IOL causing significant visual disturbance. The IOL was exchanged. Unfortunately, pathologic analysis was not performed. Patient-related factors such as hyperglycemia and hypertension might have been responsible for the opacification. To our knowledge, there is only one previous report of opacification of the Akreos Adapt AO IOL.

  13. Elementos de vulnerabilidade individual de adolescentes ao HIV/AIDS

    OpenAIRE

    Melina Mafra Toledo; Renata Ferreira Takahashi; Mónica Cecilia De-La-Torre-Ugarte-Guanilo

    2011-01-01

    A adolescência é caracterizada pela experimentação e descoberta, pelo desenvolvimento da autonomia e pela sexualidade. O objetivo desse estudo foi identificar as evidências científicas da literatura sobre os elementos da dimensão individual da vulnerabilidade de adolescentes ao HIV/AIDS. Através da revisão sistemática integrativa, em bases e bancos de dados eletrônicos (Cinahl PubMed, Scopus, LILACS, Adolec, Dedalus, BDTD e Portal de Teses da Universidade de São Paulo) rastrearam-se trabalhos...

  14. Contabilidade gerencial como apoio ao "just in time"

    OpenAIRE

    Costa, Sérgio De Carvalho

    2010-01-01

    Descreve as técnicas da filosofia "Just in Time" e apresenta as modificações necessárias aos Sistemas Gerencias para que se tornem coerentes com a filosofia JIT, bem como meçam adequadamente a melhoria contínua da Empresa. Critica tanto o Custeio' Direto como o Custeio por Absorção, apresentando como alternativa o Custeio "ABC". Mostra como o JIT facilita o Custeio dos Produtos e destaca os aspectos estratégicos da Avaliação de Desempenho. Finalmente discorre sobre um estudo de...

  15. Residual effect of a micro-encapsulated formulation of organophosphates and piriproxifen on the mortality of deltamethrin resistant Triatoma infestans populations in rural houses of the Bolivian Chaco region

    Directory of Open Access Journals (Sweden)

    Abraham Gemio Alarico

    2010-09-01

    Full Text Available The Bolivian Chaco is part of the endemic region of Chagas disease and an area where pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae populations has been reported. The World Health Organization identified these resistant populations as an important focus for research. The objective of this study was to evaluate the residual effect of a micro-encapsulated formulation containing organophosphate active ingredients and a juvenile hormone analogue (Inesfly 5A IGR on the mortality of T. infestans. Studies took place in rural houses of the Bolivian Chaco that were treated up to 34 months before and evaluated the susceptibility to pyrethroids of the offspring of field collected insects. Thirty houses were randomly selected within three communities to carry out wall bio-assays with T. infestans nymphs. Mortality was recorded 24, 48 and 72 h after wall contact. Eggs laid by females collected in the area were used to obtain first-instar nymphs and carry out pyrethroid susceptibility tests. The wall bio-assays showed that the micro-encapsulated insecticide eliminates T. infestans populations and produces detectable mortality of insects exposed to walls treated 34 months prior to the tests. The discriminant dose of deltamethrin (0.01 mg/mL showed 65% nymph survival, whereas at the highest tested dose (1.0 mg/mL 14% of the nymphs survived. These results show that Inesfly 5A IGR is an appropriate tool for the elimination of intradomestic and peridomestic populations of T. infestans resistant to pyrethroids.

  16. An Investigation on the Ecology of Triatoma vitticeps (Stal, 1859 and its Possible Role in the Transmission of Trypanosoma cruzi, in the Locality of Triunfo, Santa Maria Madalena Municipal District, State of Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Gonçalves Teresa Cristina M

    1998-01-01

    Full Text Available From January 1989 to April 1995, 465 specimens of Triatoma vitticeps were collected in the locality of Triunfo, 2nd District of Santa Maria Madalena municipal district, State of Rio de Janeiro. The bugs were found indoors by local residents with predominance of adults. The flight activity was high in hot months when the incidence in the domicile also increased. Two hundred and two bugs (111 alive and 91 dead were examined for Trypanosoma cruzi infection. This was detected in 31 of the dead bugs (34% and 88 (79% of the live bugs examined. With a view to investigate the possible vertebrate hosts of the T. cruzi isolates, the blood of 122 mammals was examined through Giemsa-stained smears, hemocultures and xenodiagnosis. T. cruzi was detected in three specimens of Didelphis marsupialis and T. (M. theileri was detected in one specimen of Bos taurus. The parasites were isolated from triatomine feces, xenoculture and hemoculture. No evidence of human infection was detected in 58 inhabitants examined, as evaluated by indirect imunofluorescence technique using T. cruzi epimastigotes as antigens. These results show that T. vitticeps is still a sylvatic species although nymphs have been found inside the domicile. Thus, an epidemiological vigilance is necessary to know the behaviour of this species following the continuous modifications promoted by the presence of man.

  17. The association of Triatoma maculata (Ericsson 1848) with the gecko Thecadactylus rapicauda (Houttuyn 1782) (Reptilia:Squamata:Gekkonidae):a strategy of domiciliation of the Chagas disease peridomestic vector in Venezuela?

    Institute of Scientific and Technical Information of China (English)

    Reyes-Lugo M; Rodrguez-Acosta A; Reyes-Contreras M; Salvi I; Gelves W; Aviln A; Llavaneras D; Navarrete LF; Cordero G; Snchez EE

    2011-01-01

    Objective: To investigate the bioecological relationship between Chagas disease peridomestic vectors and reptiles as source of feeding. Methods: In a three-story building, triatomines were captured by direct search and electric vacuum cleaner search in and outside the building. Then, age structure of the captured Triatoma maculata (T. maculata) were identified and recorded. Reptiles living in sympatric with the triatomines were also searched. Results: T. maculata were found living sympatric with geckos (Thecadactylus rapicauda) and they bit residents of the apartment building in study. A total of 1 448 individuals of T. maculata were captured within three days, of which 74.2% (1 074 eggs) were eggs, 21.5% were nymphs at different stages, and 4.3% were adults. Conclusions: The association of T. maculata and T. rapicauda is an effective strategy of colonizing dwellings located in the vicinity of the habitat where both species are present; and therefore, could have implications of high importance in the intradomiciliary transmission of Chagas disease.

  18. Modelo preditivo ao abandono do tratamento da tuberculose

    Directory of Open Access Journals (Sweden)

    Eveline de Almeida Silva

    Full Text Available Objetivou-se construir um modelo preditivo tomando como variáveis explicativas as características dos indivíduos com tuberculose para definir o tratamento mais adequado. Pesquisa do tipo observacional descritiva, de abordagem quantitativa. A população alvo (n=1925 foi retirada do Sistema de Informação de Agravos de Notificação de João Pessoa- PB, entre 2001 e 2008. No modelo final a razão de chances (OR das variáveis para o risco ao abandono foram: escolaridade em ensino médio (OR=1,56 e em ensino superior (OR=42,99, cor (OR=2,29 e reingresso após abandono (OR=2,71. A tomada de decisão deverá ser direcionada ao modelo de melhor adequação para se identificar os indivíduos passíveis de abandonar o tratamento.

  19. Illuminating Free-floating Planet Demographics with Keck AO

    Science.gov (United States)

    Henderson, Calen B.

    2017-01-01

    The frequency and mass function of free-floating planets (FFPs) are unknown. Gravitational microlensing is able to explore the demographics of FFPs, which are identifiable as short-timescale microlensing events, lasting of-order 1 day for Jupiter-mass planets. In 2011, the MOA ground-based microlensing survey group announced the discovery of an excess of short-timescale microlensing events over what was expected from Galactic models that incorporate stellar densities and kinematics. They account for this excess by positing a population of Jupiter-mass FFPs that outnumbers stars by a ratio of nearly 2:1. However, there are several other possible astrophysical explanations for short-timescale microlensing events, including bound planets on wide orbits and high-velocity stars. Although the specific events identified by MOA lack mass measurements, high-resolution imaging can determine whether the lens systems are luminous, which would exclude the FFP conclusion through proof by contradiction. We have taken H-band adaptive optics (AO) observations of the MOA FFP candidates using NIRC2 on Keck II in order to test this result. Here I will present preliminary results from these AO observations, which will help inform our understanding of the demographics of FFPs.

  20. OBSOLESCÊNCIA PROGRAMADA E TEORIA DO DECRESCIMENTO VERSUS DIREITO AO DESENVOLVIMENTO E AO CONSUMO (SUSTENTÁVEIS)

    OpenAIRE

    2012-01-01

    Inspirado em um documentário intitulado “Prêt à Jeter”, este texto tem como objetivo central colocar em apreciação algumas reflexões acerca da obsolescência programada como uma das estratégias do modelo de desenvolvimento capitalista e ensaiar uma reposta às seguintes questões: para combater a obsolescência programada se faz necessária a defesa de uma “teoria do decrescimento”? Sendo a obsolescência programada uma das estratégias da sociedade de consumo, é possível combatê-la e, ao mesmo temp...

  1. Implante Coclear: estudos concernentes à biopolítica, ao biopoder e ao biocapital em III volumes

    OpenAIRE

    Garcia, Eduardo de Campos

    2015-01-01

    Esta tese, embora aparentemente trate de assuntos concernentes ao Implante Coclear, seu objeto esbarra numa análise do que de modo visuoimperceptual autoriza a operacionalidade das modificações corporais que implicam do imbricamento entre homem e máquina: pela criação de linguagem clínico discursiva, na ordem jurídico-biológica, nas tramas interpessoais e pela própria pessoa que se autodeclara ciborgue de ouvido biônico e que acaba envolvida pela ideia da formação de outras identidades e de u...

  2. Robo-AO: autonomous and replicable laser-adaptive-optics and science system

    CERN Document Server

    Baranec, C; Ramaprakash, A N; Law, N; Tendulkar, S; Kulkarni, S; Dekany, R; Bui, K; Davis, J; Burse, M; Das, H; Hildebrandt, S; Punnadi, S; Smith, R; 10.1117/12.924867

    2012-01-01

    We have created a new autonomous laser-guide-star adaptive-optics (AO) instrument on the 60-inch (1.5-m) telescope at Palomar Observatory called Robo-AO. The instrument enables diffraction-limited resolution observing in the visible and near-infrared with the ability to observe well over one-hundred targets per night due to its fully robotic operation. Robo- AO is being used for AO surveys of targets numbering in the thousands, rapid AO imaging of transient events and longterm AO monitoring not feasible on large diameter telescope systems. We have taken advantage of cost-effective advances in deformable mirror and laser technology while engineering Robo-AO with the intention of cloning the system for other few-meter class telescopes around the world.

  3. Robo-AO: autonomous and replicable laser-adaptive-optics and science system

    Science.gov (United States)

    Baranec, C.; Riddle, R.; Ramaprakash, A. N.; Law, N.; Tendulkar, S.; Kulkarni, S.; Dekany, R.; Bui, K.; Davis, J.; Burse, M.; Das, H.; Hildebrandt, S.; Punnadi, S.; Smith, R.

    2012-07-01

    We have created a new autonomous laser-guide-star adaptive-optics (AO) instrument on the 60-inch (1.5-m) telescope at Palomar Observatory called Robo-AO. The instrument enables diffraction-limited resolution observing in the visible and near-infrared with the ability to observe well over one-hundred targets per night due to its fully robotic operation. Robo-AO is being used for AO surveys of targets numbering in the thousands, rapid AO imaging of transient events and long-term AO monitoring not feasible on large diameter telescope systems. We have taken advantage of cost-effective advances in deformable mirror and laser technology while engineering Robo-AO with the intention of cloning the system for other few-meter class telescopes around the world.

  4. The Curaçao Cohort Studies : An analysis of the implementation of combination antiretroviral therapy in Curaçao

    NARCIS (Netherlands)

    de Groot-Hermanides, Hillegonda

    2015-01-01

    In dit proefschrift geven we een overzicht van de HIV epidemie in Curaçao en analyseren we de uitkomst van antiretrovirale combinatie therapie (cART) in personen met HIV-1 infectie die behandeld worden in Curaçao. Uiteindelijk concluderen we dat de uitkomsten van cART suboptimaal zijn als gevolg van

  5. Performance of the Sewage Treatment Plant of S˜ao Jo˜ao de Iracema (Brazil

    Directory of Open Access Journals (Sweden)

    Tsunao Matsumoto

    2016-05-01

    Full Text Available Context: Some of the most important parameters measured in the sewage treatment plant –STP- are: organic matter, solids and faecal organisms. The objective of this work was to diagnose the performance of S˜ao Jo˜ao de Iracema STP for those parameters removal. Method: A batimetric survey of the anaerobic and facultative lagoons; the STP influent and effluent monitoring in three different climate seasons of 3-month long each were conducted by dissolved oxygen, pH, temperature, BOD, filtered BOD, COD, solids and coliforms measuring. Results: Sludge accumulations of 1.3 and 6.5% in the anaerobic and facultative lagoons were registered; average BOD removal was of 73.6%, smaller than the 80% recommended by Brazilian legislation; the average faecal coliform number on final effluent was of 9.55*106/100mL, above the per-missible limit of 1000/100mL. Conclusions: The STP needs to set-up a post treatment system that guarantees additional organic matter removal and fecal coliforms in order to adjust the effluent quality to the environmental legislation standards.

  6. NFIRAOS First Facility AO System for the Thirty Meter Telescope

    CERN Document Server

    Herriot, Glen; Atwood, Jenny; Boyer, Corinne; Byrnes, Peter; Caputa, Kris; Ellerbroek, Brent; Gilles, Luc; Hill, Alexis; Ljusic, Zoran; Pazder, John; Rosensteiner, Matthias; Smith, Malcolm; Spano, Paolo; Szeto, Kei; Véran, Jean-Pierre; Wevers, Ivan; Wang, Lianqi; Wooff, Robert

    2014-01-01

    NFIRAOS, the Thirty Meter Telescope's first adaptive optics system is an order 60x60 Multi-Conjugate AO system with two deformable mirrors. Although most observing will use 6 laser guide stars, it also has an NGS-only mode. Uniquely, NFIRAOS is cooled to -30 C to reduce thermal background. NFIRAOS delivers a 2-arcminute beam to three client instruments, and relies on up to three IR WFSs in each instrument. We present recent work including: robust automated acquisition on these IR WFSs; trade-off studies for a common-size of deformable mirror; real-time computing architectures; simplified designs for high-order NGS-mode wavefront sensing; modest upgrade concepts for high-contrast imaging.

  7. Correla\\c{c}\\~ao de longa-dura\\c{c}\\~ao e anal\\'ise bayesiana da evolu\\c{c}\\~ao temporal dos terremotos ao longo da falha de samambaia, nordeste do brasil

    CERN Document Server

    de Freitas, D B; Pereira, L L S; Pedro, L S; Atanazio, M G N; Vilar, C S

    2013-01-01

    A Bayesian approach is adopted to analyze the sequence of seismic events and their magnitudes near Jo\\~ao C\\^amara which occurred mainly from 1983 to 1998 along the Samambaia fault. In this work, we choose a Bayesian model for the process of occurrence times conditional on the observed magnitude values following the same procedure suggested by Stavrakakis and Tselentis (1987). The model parameters are determined on the basis of historical and physical information. We generate posterior samples from the joint posterior distribution of the model parameters by using a variant of the Metropolis-Hastings algorithm. We use the results in a variety of ways, including the construction of pointwise posterior confidence bands for the conditional intensity of the point process as a function of time, as well as, a posterior distribuition as a function of the mean of occurrence per unit time.

  8. Lévi-Strauss nos 90 voltas ao passado

    Directory of Open Access Journals (Sweden)

    Lévi-Strauss Claude

    1998-01-01

    Full Text Available Este artigo responde às críticas formuladas por Christian Delacampagne e Bernard Traimond em "A Polêmica Sartre/Lévi-Strauss Revisitada. Nas Raízes das Ciências Sociais de Hoje", publicado em Les Temps Modernes 596 (novembro-dezembro de 1997. Para fazê-lo, recorda inicialmente o caráter relativo da oposição entre "sociedades frias" e "sociedades quentes". Em seguida, refuta a tese de que Maurice Merleau-Ponty, sob a capa de um elogio, teria dirigido críticas veladas ao autor. Finalmente, reconhecendo o afastamento da antropologia de hoje em face das posições do autor, admite, sem se pronunciar sobre o valor desse movimento, que temas de ar sartriano ressurgem nos antropólogos contemporâneos.

  9. Os ditongos do latim ao português

    Directory of Open Access Journals (Sweden)

    Quednau, Laura Rosane

    2005-01-01

    Full Text Available O presente trabalho trata da evolução dos ditongos já existentes e do surgimento de novos ditongos do latim ao português, analisando a motivação de sua redução quando esta ocorre. Para tanto, apresentamos cada ditongo separadamente, trazendo informações sobre a sua forma em latim clássico, latim vulgar e português. Além disso, tratamos de outros processos de formação de ditongos em português, uma vez que o português possui um número maior de ditongos do que o latim. Por fim, apresentamos um quadro-resumo, sintetizando as informações mais importantes do trbalho: quais ditongos permaneceram, quais foram reduzidos, quais surgiram por outros processos e quais são as causas da formação de ditongos em português

  10. Do Desenho ao Design: um percurso semiótico?

    Directory of Open Access Journals (Sweden)

    Lucrécia d'Aléssio Ferrara

    2008-11-01

    Full Text Available Estudo do confronto entre as caracterí­sticas do desenho industrial como linguagem e sua possí­vel semiose no percurso que o leva a atingir o design como dimensão cognitiva que qualifica o desenho e o designer transformando-os em manifestações que interferem na dinâmica cultural do mundo urbano industrial e pós-industrial. Nesse confronto, estuda-se a ambigüidade que se pode encontrar entre estudos que utilizam a semiótica como lógica da linguagem inerente aos processos de semiose ou aqueles que a consideram um método aplicativo linear e útil í prática do projeto. Procura-se, portanto, estudar duas questões: trata-se de uma semiótica aplicada ao design ou de uma semiótica do design? Palavras-chave design, conhecimento, informação, semiótica, comunicação, desenho industrial Abstract This paper attempts at showing the confront between the characteristics of industrial design as a language and its possible semiosis that leads it to reach the design as a cognitive process. This semiosis qualifies both the drawing and the designer as cultural interferences in an industrial and postindustrial world. In this confront we examine the ambiguity that may be found between studies that makes use of semiotics as logic of the language inherent to the processes of semiosis, and those which consider it a linear applicative method, useful to the practice of the project. Thus, we aim considering two issues: is it a semiotics applied to the design or else a semiotics of the design? Key words design, knowledge, information, semiotic,communication, industrial design

  11. Infeção por Toxocara em animais de companhia: abordagem ao tratamento e ao controlo

    OpenAIRE

    Graça, Maria Inês Silva

    2015-01-01

    Toxocarose é uma doença provocada por nemátodes da família Ascarididae, da espécie Toxocara canis, no caso de cães, e Toxocara cati, no caso de gatos. Os cães e gatos desempenham um importante papel na transmissão destes parasitas ao ser humano, através da excreção de ovos nas fezes. A toxocarose é considerada uma zoonose de grande importância em Saúde Pública. Embora a espécie Toxocara cati seja considerada menos importante na transmissão desta zoonose, alguns autores defendem que Toxocara c...

  12. Assessing the vulnerability of Brazilian municipalities to the vectorial transmission of Trypanosoma cruzi using multi-criteria decision analysis.

    Science.gov (United States)

    Vinhaes, Márcio Costa; de Oliveira, Stefan Vilges; Reis, Priscilleyne Ouverney; de Lacerda Sousa, Ana Carolina; Silva, Rafaella Albuquerque E; Obara, Marcos Takashi; Bezerra, Cláudia Mendonça; da Costa, Veruska Maia; Alves, Renato Vieira; Gurgel-Gonçalves, Rodrigo

    2014-09-01

    Despite the dramatic reduction in Trypanosoma cruzi vectorial transmission in Brazil, acute cases of Chagas disease (CD) continue to be recorded. The identification of areas with greater vulnerability to the occurrence of vector-borne CD is essential to prevention, control, and surveillance activities. In the current study, data on the occurrence of domiciliated triatomines in Brazil (non-Amazonian regions) between 2007 and 2011 were analyzed. Municipalities' vulnerability was assessed based on socioeconomic, demographic, entomological, and environmental indicators using multi-criteria decision analysis (MCDA). Overall, 2275 municipalities were positive for at least one of the six triatomine species analyzed (Panstrongylus megistus, Triatoma infestans, Triatoma brasiliensis, Triatoma pseudomaculata, Triatoma rubrovaria, and Triatoma sordida). The municipalities that were most vulnerable to vector-borne CD were mainly in the northeast region and exhibited a higher occurrence of domiciliated triatomines, lower socioeconomic levels, and more extensive anthropized areas. Most of the 39 new vector-borne CD cases confirmed between 2001 and 2012 in non-Amazonian regions occurred within the more vulnerable municipalities. Thus, MCDA can help to identify the states and municipalities that are most vulnerable to the transmission of T. cruzi by domiciliated triatomines, which is critical for directing adequate surveillance, prevention, and control activities. The methodological approach and results presented here can be used to enhance CD surveillance in Brazil.

  13. HNBR/AO80共混阻尼橡胶的结构和性能%Structure and Properties of Vulcanized HNBR/AO80 Damping Rubbers

    Institute of Scientific and Technical Information of China (English)

    王爽; 张继华; 赵云峰

    2013-01-01

    在氢化丁腈橡胶(HNBR)中添加受阻酚AO80,制备HNBR/AO80共混橡胶,以改善HNBR的阻尼性能.采用DMA和FTIR等方法研究了HNBR/AO80共混硫化体系结构和性能之间的关系,并通过热处理的方法,研究了该体系的稳定性.结果表明:由于AO80的羟基与HNBR的腈基间强氢键作用,使HNBR/AO80硫化体系具有较好的阻尼性能.而热处理后,硫化体系中一些与橡胶分子有氢键作用的AO80,会不断自聚形成AO80的富相区、结晶、长大,形成大的结晶聚集体,导致共混体系的阻尼性能不稳定.%In order to improve the damping properties of hydrogenated nitrilebutadiene rubber (HNBR ) ,3,9-bis[ 1, 1-dimethyl-21 {β-(3-tertbutyl-4-hydroxy-5-methylphenyl) propionyloxy} ethyl ] -2,4,8,10-tetraoxaspi-ro [ 5, 5 ] -undecane (AO80) were added into it to prepare the vulcanized HNBR/AO80 rubbers. The relationship between the damping properties and micro-structure of this blending system was characterized by DMA & FTIR. Then the stability of this system was studied by thermal treatment at 8011. The results showed good damping property of this blending system, which was due to the effect of intermolecular hydrogen bonds between the hydroxyl groups of AO80 and the nitrile groups of HNBR. But the damping property of this curing system become unstable after thermal treated because of the self polymerization of AO80. With the increasing of thermal treated time, large number of AO80 formed rich phase,crystallized,grewed up,then formed large crystallise aggregates.

  14. Decisões judiciais sobre acesso aos medicamentos em Pernambuco

    Directory of Open Access Journals (Sweden)

    Artur Stamford

    2012-10-01

    Full Text Available OBJETIVO: Analisar as decisões do sistema jurídico sobre acesso da população a medicamentos no Sistema Único de Saúde por via judicial quanto a critérios de decisão e eventuais pressões políticas e econômicas. MÉTODOS: Estudo documental descritivo e retrospectivo de abordagem qualitativa e quantitativa. A coleta de dados foi realizada na Superintendência Estadual de Assistência Farmacêutica de Pernambuco, utilizando-se como fontes de dados 105 ações judiciais e relatórios administrativos de janeiro a junho de 2009. Verificaram-se os medicamentos com pedido ou carta patente no banco de dados do Instituto Nacional de Propriedade Industrial para identificar a frequência das patentes nas ações judiciais. Os dados obtidos foram classificados conforme o Sistema Anatômico Terapêutico Químico. Para análise das decisões judiciais, foi utilizada a teoria dos sistemas sociais autopoiéticos. RESULTADOS: As ações judiciais somaram 134 medicamentos com valor estimado de R$ 4,5 milhões para atender aos tratamentos solicitados; 70,9% dos medicamentos estavam com carta patente ou pedido e se concentraram em três classes terapêuticas: antineoplásicos e moduladores imunológicos, aparelho digestório e metabolismo, órgãos dos sentidos. Foram identificadas seis ideias centrais com os critérios de decisão dos magistrados (Constituição Federal e prescrição médica e as pressões entre os sistemas do direito, da economia e da política quanto ao acesso a medicamentos. CONCLUSÕES: A análise das decisões judiciais fundamentadas na teoria dos sistemas sociais autopoiéticos permitiu identificar as estimulações mútuas (dependências entre o sistema do direito e demais sistemas sociais em relação à questão do acesso dos cidadãos aos medicamentos. Essas dependências foram representadas pela Constituição Federal e pela propriedade intelectual. A Constituição Federal e a prescrição médica foram identificadas como

  15. Checklist of the birds of Aruba, Curaçao and Bonaire, South Caribbean

    NARCIS (Netherlands)

    Prins, T.G.; Reuter, J.H.; Debrot, A.O.; Wattel, J.; Nijman, V.

    2009-01-01

    We present an updated checklist of the birds of the islands of Aruba, Curaçao and Bonaire, and the islets of Klein Curaçao and Klein Bonaire, southern Caribbean, and compare this with earlier checklists (K.H. Voous, Stud. Fauna Curaçao Carib. Isl. 7: 1-260, 1957; Ardea 53: 205-234, 1965; Birds of th

  16. ENSINO MÉDIO ARTICULADO AO ENSINO PROFISSIONALIZANTE: NOVAS PERSPECTIVAS

    Directory of Open Access Journals (Sweden)

    S. H. S. L. M. Freire

    2012-05-01

    Full Text Available Neste artigo pretendemos discutir sobre a nova proposta do Ensino Médio articulado ao Ensino profissionalizante, nas instituições SESI e SENAI na cidade de Mossoró/RN. Essa proposta tem por finalidade proporcionar a formação integral, alicerçada nas dimensões do trabalho, ciência e cultura e ampliar as possibilidades de inserção do cidadão na vida produtiva e social, bem como dar continuidade ao seu itinerário formativo, contribuindo para a formação continuada e permanente do aluno. Dessa maneira, objetivamos com esse estudo analisar as contribuições dessa articulação para ensino - aprendizagem do aluno e as possibilidades de construção de novos conhecimentos. O estudo fundamenta-se em documentos como referenciais normativos, pedagógico, operacionais e financeiros nacionais para a articulação da educação básica do SESI com a educação profissional do SENAI (2006, relatório de mapeamento e diagnóstico do Projeto EBEP, Educação básica do SESI articulada com educação Profissional do SENAI (2001; 2011. A ênfase dada pela LDB (Lei de Diretrizes e Bases da Educação 9.394/96, para a educação profissional deixa claro que deve ser desenvolvida em articulação com o ensino regular ou por diferentes estratégias de educação continuada numa perspectiva do exercício pleno da cidadania. Esta pesquisa é resultado de um trabalho exploratório-bibliográfico que tem como objetivo maior compreender as reflexões e proposições atribuídas a essa articulação médio com o ensino profissionalizante na contemporaneidade.

  17. Robo-AO KP: A new era in robotic adaptive optics

    Science.gov (United States)

    Riddle, Reed L.; Baranec, Christoph; Law, Nicholas M.; Kulkarni, Shrinivas R.; Duev, Dmitry; Ziegler, Carl; Jensen-Clem, Rebecca M.; Atkinson, Dani Eleanor; Tanner, Angelle M.; Zhang, Celia; Ray, Amy

    2016-01-01

    Robo-AO is the first and only fully automated adaptive optics laser guide star AO instrument. It was developed as an instrument for 1-3m robotic telescopes, in order to take advantage of their availability to pursue large survey programs and target of opportunity observations that aren't possible with other AO systems. Robo-AO is currently the most efficient AO system in existence, and it can achieve an observation rate of 20+ science targets per hour. In more than three years of operations at Palomar Observatory, it has been quite successful, producing technology that is being adapted by other AO systems and robotic telescope projects, as well as several high impact scientific publications. Now, Robo-AO has been selected to take over operation of the Kitt Peak National Observatory 2.1m telescope. This will give Robo-AO KP the opportunity to pursue multiple science programs consisting of several thousand targets each during the three years it will be on the telescope. One-sixth of the observing time will be allocated to the US community through the NOAO TAC process. This presentation will discuss the process adapting Robo-AO to the KPNO 2.1m telescope, the plans for integration and initial operations, and the science operations and programs to be pursued.

  18. Isobolographic analysis of interactions among losigamone analog AO-620 and two conventional antiepileptic drugs.

    Science.gov (United States)

    Borowicz, Kinga K; Jaszczyk, Bozena; Czuczwar, Stanisław J

    2005-01-01

    In the present study, we investigated pharmacodynamic interactions among AO-620, a losigamone analog, and two conventional antiepileptic drugs, valproate (VPA) and phenobarbital (PB). Experiments were conducted in the maximal electroshock test in mice. Isobolographic analysis of the obtained data revealed pure additive interactions between AO-620 and PB applied at three dose ratios of 1:1, 1:3 and 3:1. Antagonism was observed when AO-620 was co-administered with VPAat the ratio of 3:1, while additive interactions were seen in two remaining proportions (1:3 and 1:1). Surprisingly, the interaction pattern of AO-620 appeared quite different from that of losigamone.

  19. Do Antissocialismo ao Anticapitalismo: um estudo sobre a Rerum Novarum

    Directory of Open Access Journals (Sweden)

    Claudinei Magno Magre Mendes

    2016-05-01

    Full Text Available O objetivo deste artigo é examinar o processo de constituição do anticapitalismo por meio da análise da Rerum Novarum, a primeira encíclica social. Comparando esta com as encíclicas anteriores do século XIX, pudemos estabelecer o que ela trouxe de novidade no que diz respeito ao combate que a Igreja travava contra o socialismo. Com essa comparação, foi possível caracterizar a postura anticapitalista, confrontando a nossa maneira de concebê-la com as demais. Para o exame da Rerum Novarum foi igualmente importante analisar alguns estudos referentes a ela, tanto os que apareceram logo após a sua publicação como os que foram realizados em décadas mais recentes. Como conclusão, definimos o anticapitalismo como uma atitude que somente poderia ter surgido em condições sociais e políticas bastante particulares e que apenas apareceram em fins do século XIX.

  20. Revendo estudos sobre a assistência domiciliar ao idoso

    Directory of Open Access Journals (Sweden)

    Andreza Aparecida de Lima

    2013-06-01

    Full Text Available A assistência domiciliar apresenta-se como uma recente modalidade de cuidado aos idosos, sobretudo no Brasil, e vem sendo inserida nas políticas públicas. Este estudo busca identificar produções científicas acerca da assistência domiciliar, a fim de verificar quais as contribuições desta na dinâmica social do idoso. A pesquisa foi realizada nas bases de dados LILACS e MEDLINE, com artigos publicados entre os anos de 2001 a 2011, nos idiomas português e inglês. A combinação de palavras-chave utilizada foi home care e elderly, elderly e care, e elderly e caregiver, e as correspondentes em português. A discussão dos resultados evidenciou a importância da assistência domiciliar, principalmente para idosos solitários ou negligenciados. A revisão revelou poucos estudos referentes à assistência domiciliar na dinâmica social de idosos e a necessidade de ampliar as discussões para além do campo da saúde.

  1. Elementos de vulnerabilidade individual de adolescentes ao HIV/AIDS

    Directory of Open Access Journals (Sweden)

    Melina Mafra Toledo

    2011-04-01

    Full Text Available A adolescência é caracterizada pela experimentação e descoberta, pelo desenvolvimento da autonomia e pela sexualidade. O objetivo desse estudo foi identificar as evidências científicas da literatura sobre os elementos da dimensão individual da vulnerabilidade de adolescentes ao HIV/AIDS. Através da revisão sistemática integrativa, em bases e bancos de dados eletrônicos (Cinahl PubMed, Scopus, LILACS, Adolec, Dedalus, BDTD e Portal de Teses da Universidade de São Paulo rastrearam-se trabalhos publicados entre 1996 e 2006. Compuseram a amostra final 41 estudos. O elemento da vulnerabilidade individual mais freqUente nos estudos foi o grau e qualidade do conhecimento sobre HIV/AIDS. A revisão permitiu identificar evidências científicas da vulnerabilidade individual relevantes para o planejamento das ações de prevenção à infecção pelo HIV em adolescentes.

  2. Touraine, Burawoy, Gramsci: do social ao político

    Directory of Open Access Journals (Sweden)

    Leonardo Mello e Silva

    Full Text Available O ensaio empreende uma discussão da proposta da sociologia pública de Michael Burawoy, nos anos 2000, a partir da confrontação com a intervenção sociológica de Alain Touraine, formulada nos anos 1970. Pontos de contato são detectados no que diz respeito à relação entre pesquisador e pesquisado, como atores sociais. Problemas e limitações, tanto da intervenção sociológica quanto da sociologia pública, são elencados, trazendo-se como apoio exemplos extraídos de trabalho de campo próprio. O texto vale-se também da inspiração de Antonio Gramsci e sua concepção da relação entre senso comum e intelectuais. Ao invés de um juízo conclusivo, propõe-se um diálogo entre os autores, todos preocupados, à sua maneira, com o destino público ou cívico do conhecimento obtido sobre o mundo social.

  3. Introdução ao Manual de Epicteto

    Directory of Open Access Journals (Sweden)

    Aldo Lopes Dinucci

    2012-01-01

    Full Text Available Trata-se da edição de divulgação de tradução do Manual de Epicteto realizada por Aldo Dinucci e Alfredo Julien. Epicteto, filósofo estoico grego nascido no ano 55 em Hierápolis, na Frígia, Ásia Menor (hoje Turquia, como Sócrates, nada escreveu. Seu pensamento nos chegou através de seu aluno Flávio Arriano (cidadão romano de origem grega, que compilou (possivelmente com auxílio da taquigrafia suas aulas em oito livros (As Diatribes de Epicteto, das quais quatro livros sobrevivem e constituiu o Encheirídion, termo que em grego significa "punhal, arma portátil ou livro portátil, manual". Este pequeno trabalho foi composto por Arriano tomando por base suas compilações das aulas de Epicteto. Consistindo num conjunto de apotegmas para que o seguidor do estoicismo tenha sempre ao alcance da mão os princípios para enfrentar as dificuldades da vida e vencê-las, o Encheirídion tornou célebre o nome de Epicteto.

  4. VALORES DO RNI DE PACIENTES SUBMETIDOS AO TRATAMENTO COM ANTICOAGULANTES

    Directory of Open Access Journals (Sweden)

    Simone Aparecida Matos de SOUZA

    2015-12-01

    Full Text Available Uma pesquisa exploratória de natureza qualitativa e quantitativa foi realizada durante o período de maio a agosto de 2013 no Laboratório de Análises Clínicas do Hospital das Clínicas localizado no município de Itaperuna, RJ. O objetivo foi analisar o perfil dos pacientes sob tratamento com anticoagulantes, demonstrando as potenciais variações nos valores da Relação Normalizada Internacional (RNI em comparação aos valores de referência bem como a frequência de realização de tais exames. Um questionário foi aplicado pouco antes da coleta do sangue, estabelecendo-se como critério de inclusão o uso de medicamentos anticoagulantes. Os resultados indicaram um total de 45 pacientes sob tratamento com anticoagulantes, sendo 32 do sexo feminino e 13 do sexo masculino. A maioria dos pacientes encontrava-se sob tratamento com o anticoagulante de uso oral varfarina e se encontrava com idade superior a 50 anos. O valor médio do RNI apresentou-se dentro dos limites considerados seguros, porém mais da metade dos pacientes apresentou RNI < 2,0 e a maioria dos pacientes sob tratamento com anticoagulantes não realiza a avaliação do RNI com frequência.

  5. Uma abordagem ao sentimento moral na filosofia kantiana

    Directory of Open Access Journals (Sweden)

    Cláudia Maria Fidalgo da Silva

    2014-11-01

    Full Text Available O principal objectivo do presente artigo é apresentar uma abordagem ao sentimento moral na filosofia kantiana.1 Embora Kant entenda que o ser humano não deva agir considerando o afecto Affekt e a paixão , tal não significa, como por vezes é considerado, que o autor defenda que a acção virtuosa não deva ser acompanhada por qualquer sentimento. Nesta linha, o artigo possui três etapas principais: 1 realçar o que Kant entende por afecto; 2 evidenciar a posição kantiana sobre a paixão; 3 investigar os sentimentos morais apresentados pelo autor, que surgem na “Introdução à doutrina da virtude”: 3.1 sentimento moral; 3.2 consciência moral ; 3.3 filantropia ; 3.4 respeito . Pretender-se-á apontar para o facto deles, em última análise, se resumirem a um único sentimento, o sentimento de respeito à lei.

  6. Subsunção do trabalho imaterial ao capital

    Directory of Open Access Journals (Sweden)

    Eleutério F. S. Prado

    Full Text Available O presente artigo examina a transformação do modo de produção capitalista que acompanha a expansão do trabalho imaterial como fonte de geração de riqueza efetiva. Essa avaliação é feita no interior da teoria do valor-trabalho e da crítica da economia política. Para tanto, interroga, primeiro, o verdadeiro impacto da informatização na natureza capitalista dos processos produtivos atualmente em andamento, principalmente nos países centrais. Compara, depois, as principais características da indústria taylorista com as características marcantes da indústria pós-taylorista. Ao final, conclui que uma transformação importante do sistema capitalista está em curso: a grande indústria, tal como foi caracterizada por Marx em O Capital, não predomina mais nos países do centro do sistema, pois o que aí prevalece agora é a pós-grande indústria.

  7. INTRODUÇAO AOS MODELOS ODE PARTICIPAÇÃO DE MERCADO EM SERVIÇOS DE TRANSPORTES

    Directory of Open Access Journals (Sweden)

    Antonio Galvão Novaes

    2010-04-01

    Full Text Available

    Análises de participação de mercado ("market share" são comuns em estudos na área de "marketing", envolvendo produtos e respectivas marcas, de um lado, o perfil da demanda de outro, e o posicionamento das empresas produtoras que competem nesse mercado, num terceiro bloco. No setor de transportes a literatura registra aplicações em ligações aéreas e em serviços intermunicipais de ônibus. Os modelos de participação de mercado se aplicam normalmente a situações em que os operadores oferecem serviços de transportes similares num determinado mercado, com a competição sendo feita não com base em atributos modais intrínsecos, mas sim apoiada em características diferenciadoras intra-modais mais tênues, tais como conforto, atendimento, promoções tarifárias, "marketing" e propaganda, etc. Neste artigo é feita uma introdução aos modelos de "market share" em transportes, com a apresentação de um exemplo de aplicação ao transporte intermunicipal de passageiros em ônibus, sendo discutidos também aspectos ligados a calibração e tais modelos.

    ABSTRACT

    Market share analysis is common in marketing studies involving products and respective brands, on one hand, the demand profile on the other hand, and the competiting industries on a third set. In the transport sector the literature registers a number of applications to air transport problems and to intercity bus services. Market share models are normally applied to situations in which operators offer, in a specific market, transport services that are similar in nature, with competition based not on intrinsic modal attributes, but rather on more tenous intra-mode differentiating characteristics, such as comfort, attendance, tariff promotions, marketing and advertising efforts, etc (sub-modal configurations. It is presented in this paper an introduction to market share modelling in transport, with an example concerning an intercity bus service in

  8. Status Update and Closed-Loop Performance of the Magellan Adaptive Optics VisAO Camera

    CERN Document Server

    Kopon, Derek; Males, Jared; Gasho, Victor; Morzinski, Katie; Follette, Katherine

    2014-01-01

    We present laboratory results of the closed-loop performance of the Magellan Adaptive Optics (AO) Adaptive Secondary Mirror (ASM), pyramid wavefront sensor (PWFS), and VisAO visible adaptive optics camera. The Magellan AO system is a 585-actuator low-emissivity high-throughput system scheduled for first light on the 6.5 meter Magellan Clay telescope in November 2012. Using a dichroic beamsplitter near the telescope focal plane, the AO system will be able to simultaneously perform visible (500-1000 nm) AO science with our VisAO camera and either 10 micron or 3-5 micron science using either the BLINC/MIRAC4 or CLIO cameras, respectively. The ASM, PWS, and VisAO camera have undergone final system tests in the solar test tower at the Arcetri Institute in Florence, Italy, reaching Strehls of 37% in i'-band with 400 modes and simulated turbulence of 14 cm ro at v-band. We present images and test results of the assembled VisAO system, which includes our prototype advanced Atmospheric Dispersion Corrector (ADC), prot...

  9. The nature of ethnic identity among the people of Curaçao

    NARCIS (Netherlands)

    Dijs, N.M. van der

    2011-01-01

    This qualitative research explores the nature of ethnic identity among the people of Curaçao. The questions that are of fundamental interest to this study are: how do the people of Curaçao perceive themselves ethnically, how do they experience their sense of belonging to an ethnic group and what fac

  10. Optimal control strategy to reduce the temporal wavefront error in AO systems

    NARCIS (Netherlands)

    Doelman, N.J.; Hinnen, K.J.G.; Stoffelen, F.J.G.; Verhaegen, M.H.

    2004-01-01

    An Adaptive Optics (AO) system for astronomy is analysed from a control point of view. The focus is put on the temporal error. The AO controller is identified as a feedback regulator system, operating in closed-loop with the aim of rejecting wavefront disturbances. Limitations on the performance of

  11. Patterns of Impairments in AOS and Mechanisms of Interaction between Phonological and Phonetic Encoding

    Science.gov (United States)

    Laganaro, Marina

    2012-01-01

    Purpose: One reason why the diagnosis of apraxia of speech (AOS) and its underlying impairment are often debated may lie in the fact that most patients do not display pure patterns of AOS. Mixed patterns are clearly acknowledged at other levels of impairment (e.g., lexical-semantic and lexical-phonological), and they have contributed to debate…

  12. Data of evolutionary structure change: 1AO5B-1ELCA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1AO5B-1ELCA 1AO5 1ELC B A VVGGFNCEKNSQPWQVAVYYQ----KEHICGGVLLDRNW...TPTWQKPDDLQCVFITLLPNENCAKV--YLQKVTDVMLCAGEMGGGKDTCRDDSGGPLICD----GILQGTTSYGPV-PCGKPGVPAIYTNLIKFNSWIKDTMMKNA TRP CA 491 1ELC A 1ELCA...hain> 1ELC A 1ELCA PLHCLVNGQYAVHG... PRO CA 225 1ELC A 1ELCA

  13. LITERATURA E CULTURA: DO NACIONAL AO TRANSNACIONAL, DE JOSÉ LUÍS JOBIM

    OpenAIRE

    Anita Martins Rodrigues de Moraes

    2015-01-01

    Reviewed book:JOBIM, José Luís. Literatura e cultura: do nacional ao transnacional. Rio de Janeiro: Eduerj, 2013. Livro resenhado:JOBIM, José Luís. Literatura e cultura: do nacional ao transnacional. Rio de Janeiro: Eduerj, 2013.

  14. Use of AO PSF models for the Study of Resolved Stellar Populations

    NARCIS (Netherlands)

    Deep, A.; Fiorentino, G.; Jolissaint, L.; Tolstoy, E.; Clénet, Y.; Conan, J.-M.; Fusco, Th.; Rousset, G.

    2010-01-01

    The full scientific exploitation of AO images to study resolved stellar populations is still in a nascent stage. This requires pushing to the faint limits and carrying out deep and accurate crowded field photometry and astrometry. The main complexity of AO images is that the correction is never perf

  15. O sindicalismo mineiro frente aos desafios dos anos 90

    Directory of Open Access Journals (Sweden)

    Antônio Carvalho Neto

    2000-12-01

    Full Text Available O que se analisa neste artigo é como os sindicatos dos bancários, metalúrgicos e trabalhadores em telecomunicações têm negociado o (desemprego, a demanda por qualificação e a remuneração variável. Foram tabuladas/analisadas 3.569 cláusulas de acordos coletivos, e realizadas 26 entrevistas com dirigentes sindicais e empresariais em três Estados (RJ, SP e MG. Os resultados indicam que as mudanças organizacionais foram incomparavelmente mais negociadas no setor metalúrgico do ABC. A flexibilização da jornada foi trocada pela redução (da jornada sem redução de salário nos setores metalúrgicos paulistas, mas não em Minas Gerais. No setor bancário, houve poucos resultados nas negociações sobre jornada. As negociações em torno da remuneração variável vinculada às metas das empresas aconteceram nos setores metalúrgicos, e não nos setores de serviços. As entrevistas apontam para um sindicalismo mais propositivo, no sentido de uma intervenção negociada, predisposição que não é correspondida pelos empresários, grosso modo. O Estado, que poderia utilizar seu poder regulador para induzir os empresários a negociarem nessa direção não tem produzido resultados satisfatórios. Conclui-se que não há maturidade empresarial no Brasil que permita ao Estado ausentar-se das relações de trabalho.

  16. Robo-AO: Initial results from the first autonomous laser guide star adaptive optics instrument

    Science.gov (United States)

    Riddle, R. L.; Baranec, C.; Law, N. M.; Ramaprakash, A. N.; Tendulkar, S.; Hogstrom, K.; Bui, K.; Burse, M.; Chordia, P.; Das, H.; Dekany, R.; Kulkarni, S.; Punnadi, S.; Smith, R.

    2014-12-01

    Large surveys are discovering thousands of objects which require further characterization at high angular resolution. The demands on space-based observatories and large telescopes with AO systems leave them generally unavailable for large high angular resolution surveys. To address this gap, we have developed Robo-AO, the first robotic laser AO system, as an economical and efficient imaging instrument for 1-3 m class telescopes. Observations of over 200 stellar objects per night have routinely been performed, with target-to-target observation overheads of less than 1.5 minutes. Scientific programs of several thousands of targets can be executed in mere weeks, and Robo-AO has already completed the three largest AO surveys to date.

  17. Lick Observatory's Shane telescope adaptive optics system (ShaneAO): research directions and progress

    Science.gov (United States)

    Gavel, Donald T.; Kupke, Renate; Rudy, Alexander R.; Srinath, Srikar; Dillon, Daren; Poyneer, Lisa A.

    2016-07-01

    We present a review of the ongoing research activity surrounding the adaptive optics system at the Shane telescope (ShaneAO) particularly the R&D efforts on the technology and algorithms for that will advance AO into wider application for astronomy. We are pursuing the AO challenges for whole sky coverage diffraction-limited correction down to visible science wavelengths. This demands high-order wavefront correction and bright artificial laser beacons. We present recent advancements in the development of MEMS based AO correction, woofer-tweeter architecture, wind-predictive wavefront control algorithms, atmospheric characterization, and a pulsed fiber amplifier guide star laser tuned for optical pumping of the sodium layer. We present the latest on-sky results from the new AO system and present status and experimental plans for the optical pumping guide star laser.

  18. Encontro de Panstrongylus megistus em ecótopo artificial: domiciliação ou mera visitação?

    Directory of Open Access Journals (Sweden)

    Nascimento Carlos

    1997-01-01

    Full Text Available No combate à doença de Chagas no Brasil, foi utilizado como primeira medida o controle químico, erradicando o Triatoma infestans, o mais importante vetor, tal combate vem favorecendo o aparecimento de vetores secundários, como Triatoma sordida and Panstrongylus megistus, espécies que podem eventualmente ser encontradas no domicílio, como os triatomíneos provenientes de Bernardino de Campos e Sete Barras por nós examinados que foram encontrados no domicílio e positivos para o T. cruzi, sugerindo que, apesar da doença de Chagas estar controlada no Estado de São Paulo, existe a necessidade de aprimorar os conhecimentos sobre o comportamento destes vetores para que mudanças nas medidas de controle sejam introduzidas.

  19. Bringing the Visible Universe into Focus with Robo-AO

    Science.gov (United States)

    Baranec, Christoph; Riddle, Reed; Law, Nicholas M.; Ramaprakash, A. N.; Tendulkar, Shriharsh P.; Bui, Khanh; Burse, Mahesh P.; Chordia, Pravin; Das, Hillol K.; Davis, Jack T. C.; Dekany, Richard G.; Kasliwal, Mansi M.; Kulkarni, Shrinivas R.; Morton, Timothy D.; Ofek, Eran O.; Punnadi, Sujit

    2013-02-01

    focus a high-power laser beam in the direction of the astronomical target to create an artificial reference of known shape, also known as a 'laser guide star'. The Robo-AO laser adaptive optics system employs a 10-W ultraviolet laser focused at a distance of 10 km to generate a laser guide star. Wavefront sensor measurements of the laser guide star drive the adaptive optics correction resulting in diffraction-limited images that have an angular resolution of ~0.1 arc seconds on a 1.5-m telescope.

  20. Avaliação da qualidade de vida e vulnerabilidade ao estresse no contexto hospitalar

    Directory of Open Access Journals (Sweden)

    Fernanda Ottati

    2013-05-01

    Full Text Available O conceito de Qualidade de Vida é usado para medir as condições de vida de um ser humano, já a palavra vulnerabilidade refere-se à chance das pessoas estarem expostas ao adoecer. Objetivou-se analisar possíveis relações entre percepção de qualidade de vida e a vulnerabilidade ao estresse no trabalho em enfermeiros, técnicos e auxiliares de enfermagem que atuam na UTI e no setor de emergência de um hospital público. Utilizou-se o instrumento de qualidade de vida da Organização Mundial da Saúde, WHOQOL-Bref e a Escala de Vulnerabilidade ao Estresse no Trabalho. Encontrou-se, em relação ao setor de trabalho, diferença significativa apenas para Qualidade de vida total. Portanto pode-se afirmar que os profissionais que atuam na UTI possuem maior percepção de qualidade de vida quando comparados aos do SUS. Na correlação entre os dois instrumentos, o domínio meio ambiente do WHOQOL-Bref correlacionou-se negativa e significativamente com todos os fatores da EVENT, indicando que a percepção da pessoa em relação aos diversos aspectos do meio em que vive tem relação com a vulnerabilidade ao estresse.

  1. Robo-AO: The First Autonomous Laser Guide Star Adaptive Optics System for Small Telescopes

    Science.gov (United States)

    Riddle, Reed L.; Baranec, C.; Ramaprakash, A. N.; Law, N.; Tendulkar, S.; Kulkarni, S.; Bui, K.; Burse, M.; Chordia, P.; Das, H.; Dekany, R.; Kasliwal, M.; Ofek, E.; Zolkower, J.

    2011-01-01

    Robo-AO will be the first fully autonomous laser guide star adaptive optics and science system. Specifically designed to take advantage of small (1 to 3 meter) telescopes, Robo-AO will deliver high angular resolution science in the visible and near infrared for up to hundreds of targets per night. This will enable the exploration of science programs not practical for larger aperture adaptive optics systems. This presentation discusses the current status of the Robo-AO project, including the laboratory testbed, laser guide star facility and plans for a demonstration of the fully autonomous system next year.

  2. Adesão ao uso de medicamentos entre idosos hipertensos

    OpenAIRE

    Aiolfi,Claucia Raquel; ALVARENGA, Márcia Regina Martins; Moura,Cibele de Sales; Renovato,Rogério Dias

    2015-01-01

    INTRODUÇÃO: O crescimento da população idosa está associado à elevada prevalência de doenças crônicas. Tal fato favorece a exposição dessa população ao uso de múltiplos medicamentos, e também ao aumento de incapacidades físicas e mentais, trazendo desafios para as famílias e sociedade. OBJETIVO: Descrever a adesão ao uso de medicamentos em idosos hipertensos com déficit cognitivo, assistidos pela Es...

  3. Simulation for CCSDS Advanced Orbiting System (AOS) with BONeS Designer

    Institute of Scientific and Technical Information of China (English)

    马永奎; 张中兆; 张乃通

    2003-01-01

    This paper describes the modeling and simulation of the protocol of CCSDS advanced orbiting systems (AOS). The network features modeled in the implementation of CCSDS AOS are to multiplex different kinds of sources into virtual channel data units (VCDUs) in the data processing module. The emphasis of this work is placed on the algorithm for commutating VCDUs into physical channels in the form of continuous data stream. The objectives of modeling CCSDS AOS protocol are to analyze the performance of this protocol when it is used to process various data.

  4. On-sky speckle nulling demonstration at small angular separation with SCExAO

    CERN Document Server

    Martinache, Frantz; Jovanovic, Nemanja; Clergeon, Christophe; Singh, Garima; Kudo, Tomoyuki; Currie, Thayne; Thalmann, Christian; McElwain, Michael; Tamura, Motohide

    2014-01-01

    This paper presents the first on-sky demonstration of speckle nulling, which was achieved at the Subaru Telescope in the context of the Subaru Coronagraphic Extreme Adaptive Optics (SCExAO) Project. Despite the absence of a high-order high-bandwidth closed-loop AO system, observations conducted with SCExAO show that even in poor-to-moderate observing conditions, speckle nulling can be used to suppress static and slow speckles even in the presence of a brighter dynamic speckle halo, suggesting that more advanced high-contrast imaging algorithms developed in the laboratory can be applied to ground-based systems.

  5. Marco regulatórios sobre o acesso ao patrimônio genético e ao conhecimento tradicional associado no Brasil

    OpenAIRE

    2015-01-01

    O objetivo geral deste trabalho foi elaborar um manual sobre o acesso ao Patrimônio Genético (PG) e ao Conhecimento Tradicional Associado (CTA), tendo como base a legislação brasileira vigente, com o intuito de disseminar estas informações para pesquisadores, docentes, gestores e toda a comunidade acadêmica, em especial, da Universidade Federal do Triângulo Mineiro – UFTM, a ser divulgado no site do Núcleo de Inovação Tecnológica (NIT) da Universidade. O trabalho possui uma temática inter e m...

  6. Contribuição ao estudo da hipsarritmia

    Directory of Open Access Journals (Sweden)

    Ana Maria Fisch

    1966-03-01

    Full Text Available Resumindo as principais contribuições que resultaram na conceituação atual da hipsarritmia, destacam os autores a noção de que não constitui ela uma entidade clínica determinada, mas uma sindrome eletroclínica especial, própria da primeira infância, e cujo diagnóstico só pode ser firmado à luz do traçado eletrencefalográfico característico. Analisam a variedade de fatôres etiológicos à maneira do que ocorre com as encefalopatias infantis em geral, chamando a atenção para a importância do fator etário, das condições traumáticas e do problema da anóxia cerebral neonatorum. Necessária ênfase é conferida ao fenômeno da maturação encefálica que explica, segundo o consenso dos autores modernos, as características peculiares da síndrome. A hipsarritmia pode instalar-se em indivíduo previamente são ou em encefalopata, tendo evolução variável, pois tanto pode evolver favoràvelmente, o que é mais raro, como modificar-se, seja no sentido da epilepsia focal ou outra forma de epilepsia, o que é mais comum, ou, então, permanecer inalterada. Essa evolução - a história natural da hipsarritmia - é apreciada especialmente sob o aspecto eletrencefalográfico, o qual pode, ou não, ser acompanhado de correspondente evolução clínica. A sintomatologia é variável, sendo de notar a grande dominância das crises de espasmos em flexão. Sendo pobre o subsídio anátomo-patológico da condição, os autores, a êsse propósito, apenas acrescentam os dados referentes à necropsia de um dos seus casos, com achados inespecíficos. O prognóstico, na maioria das vêzes mau, pode variar, especialmente após o uso do ACTH. O tratamento é difícil, freqüentemente desapontador. As melhores respostas são obtidas com o ACTH e, algumas vêzes, com os antibióticos e medicação sintomática, porém, quase sempre reaparece a sintomatologia, se suprimida a medicação que, por outro lado, nem sempre pode ser continuada.

  7. Das montanhas mexicanas ao ciberespaço

    Directory of Open Access Journals (Sweden)

    Pedro Henrique Falco Ortiz

    2005-12-01

    Full Text Available ESTE ARTIGO faz uma apanhado histórico contextualizado e uma análise comunicacional sobre o Exército Zapatista de Libertação Nacional - EZLN, que apareceu publicamente no estado mexicano de Chiapas em janeiro de 1994, a partir de sua origem indígena, com as demandas seculares dessa parcela da população marginalizada, suas peculiaridades e sua prática política, que o fazem um grupo armado bastante original dentro do contexto das lutas populares na América Latina. As estratégias políticas e de comunicação do EZLN são diferenciais importantes em relação a outras experiências guerrilheiras anteriores. Assim, analisamos também a habilidade que os zapatistas tiveram - e ainda têm - para conquistar espaço nos meios de comunicação convencionais e criar uma eficiente rede de solidariedade e comunicação, combinando as tradições seculares das comunidades indígenas, que são suas bases, aos recursos tecnológicos de meios eletrônicos como a Internet e a Web. Esta combinação criativa de iniciativas políticas bem-sucedidas, bases sociais resistentes, diálogo permanente com a chamada sociedade civil - nacional e internacional - e vitórias importantes no campo da comunicação, compensam a fragilidade militar do EZLN e possibilitam sua continuidade, mesmo no contexto militar bastante adverso em que se situa o conflito chiapaneco.THE PRESENT article is an historical and contextualized communicational analisis on the Zapatista National Liberation Army - EZLN, that came to light the first time on january,1994, in the mexican southern state of Chiapas, with its indigenous origin, ancient struggles of the marginalized population, its own culture and political praxis that made it a very particular social movement in the context of Latin America. The polictical and communication strategies of the EZLN are important changes when we compare it to other guerrilla movements. We also analize the hability and creativity the zapatistas have

  8. Potencialidades e espaços ao empreendedorismo

    Directory of Open Access Journals (Sweden)

    Adriano Lago

    2008-01-01

    Full Text Available A criação de negócios é apontada como uma das causas da prosperidade de um local, região, estado ou de um país. É neste sentido que o empreendedorismo tem recebido destaque nos últimos anos, como promotor de novos negócios. Captar, descrever e analisar o fenômeno do empreendedorismo é, portanto, necessário para o desenho de ações de promoção do desenvolvimento. Assim, o presente estudo aborda o empreendedorismo na piscicultura, tomando como base três casos analisados: uma propriedade com rizipiscicultura, uma com piscicultura de ciclo completo inclusive com frigorífico, e outra onde a piscicultura desenvolve-se através de um pesque-pague, com vistas a proporcionar o turismo rural. Para fins de pesquisa, entrevistaram-se os proprietários de cada estabelecimento na busca dos dados necessários para a construção de uma abordagem consistente da temática proposta, ou seja, identificar a presença do empreendedorismo e suas características particulares em cada propriedade analisada. Verificou-se que determinadas características empreendedoras são comuns aos três casos analisados: habilidade para identificar oportunidades; busca de conhecimento de sua área de atuação; senso de organização; disposição para tomar decisões; capacidade de liderança; talento para empreender; otimismo e tino empresarial; ser estrategista, além do dinamismo. Portanto, foi possível observar que o perfil empreendedor dos proprietários de cada negócio tornou possível a transformação de idéias e problemas em oportunidades reais de negócio.Abstract The business opening is indicated as one of the prosperity causes of a country, state, region or local. It’s in this context that the entrepreneurship has received attention along the last years as new business promoter. This way, to study and promote development actions it’s necessary, firstly, to get, to describe and to analyze the entrepreneurship phenomenon by itself. So, this study

  9. LDEF (Prelaunch), AO201 : Interplanetary Dust Experiment, Tray B12

    Science.gov (United States)

    1984-01-01

    LDEF (Prelaunch), AO201 : Interplanetary Dust Experiment, Tray B12 The prelaunch photograph shows the six (6) inch deep Interplanetary Dust Experiment (IDE) master control tray. The tray has three (3) mounting/cover plates elevated on fiberglass stand-offs to provide clearance and protection for hardware and electronics located underneath. The stand-offs also raise the plates to a level that minimizes shading of detectors by the tray sidewalls. The mounting plate located at the left hand end of the tray is populated with eighty (80) metaloxide-silicon (MOS) capacitor-type impact sensors and one (1) solar sensor that is located approximately in the center of the mounting plate. The IDE sensors are two (2) inch diameter MOS capacitor structures approximately 250 um thick. The detectors are formed by growing either 0.4um or 1.0um thick silicon oxide, SiO2, layer on the 250um thick, B-doped polished silicon wafer. The top metal contact, the visible surface, was formed by vapor deposition of 1000A of aluminum on the SiO2 surface. Aluminum was also vapor deposited on the backside to form the contact with the silicon substrate. Gold wires are bonded to the front and back aluminum layers for use in connecting the detectors to the circuits. The complete wafers, IDE detectors, are mounted on chromic anodized aluminum frames by bonding the detector backside to the aluminum frame with a space qualified RTV silicon adhesive, de-volatized RTV-511. The difference in colors of the detectors is caused by reflections in the metallized surfaces. A reflection of one of the technicians is visible in the three (3) rows of detector on the left hand side of the mounting plate. The solar sensor, located at the mounting plate center, consist of four (4) silicon solar cells connected in series and associated circuity bonded to an aluminum baseplate. The solar sensor registered each orbital sunrise independant of LDEF orientation at the time of sunrise. When IDE solar sensor data from the six

  10. Contribuição ao estudo da Tripanosomiase Americana

    Directory of Open Access Journals (Sweden)

    Bichat de Almeida Rodrigues

    1942-01-01

    ano (época do estío. 6 Em plena mata, n’uma toca de tamanduá (T. tetradactylus foram encontradas larvas, ninfas e adultos de P. geniculatus. A casa situada mais próximo desse foco, foi a que apresentou maior infestação (22 exemplares e exclusiva para essa espécie. Em uma toca de macaco da noite (P. flavus, foi encontrada uma larva de Triatomídeo. Este foco também ficava próximo à casa acima referida. Em toca de P. flavus foi também achado um exemplar adulto de Panstrongylus refotuberculatus. 7 Amostras de S. cruzi isoladas de animais silvestres, mostraram fraco poder infectante. A amostra isolada do cão, embora infectando facilmente os animais de laboratório, pelos estudos biométricos feito por DIAS e FREITAS, afasta-se das amostras humanas típicas. 8 São discutidos os resultados acima referidos e, pelos hábitos dos transmissores, pela predominância de depositários silvestres do parasito, conclue-se pela natureza silvestre da Tripanosomiase Americana no local estudado. Se bem que não tenham sido verificadas infecções humanas, dado o encontro de um cão parasitado – infecção esta que se pode ter verificado pelo meio normal da transmissão da moléstia – admite-se a possibilidade do aparecimento de casos humanos nessa região. Ressalta-se a confirmação que tais resultados parecem trazer á hipótese de CARLOS CHAGAS, que pensava ser esta doença primitivamente silvestre, com posterior adaptação aos animais domésticos e ao homem.1 The epidemiology of Chagas’ disease was studied for the first time in a systematized work, in a district of the region of the Amazon estuary, whose ecological conditions differ from those encountered in other zones where similar researches have been made. 2 At the place chosen for the studies – Aurá – a locality about 10 kms from Belem (State of Pará, no human infection by S. cruzi has been found, either by blood examination or xenodiagnosis, both having been tried on all the inhabitants (117 people

  11. Robo-AO: An Autonomous Laser Adaptive Optics and Science System

    Science.gov (United States)

    Baranec, Christoph; Riddle, Reed; Ramaprakash, A. N.; Law, Nicholas; Tendulkar, Shriharsh; Kulkarni, Shrinivas; Dekany, Richard; Bui, Khanh; Davis, Jack; Zolkower, Jeff; Fucik, Jason; Burse, Mahesh; Das, Hillol; Chordia, Pravin; Kasliwal, Mansi; Ofek, Eran; Morton, Timothy; Johnson, John

    2011-07-01

    Robo-AO, a fully autonomous, laser guide star adaptive optics and science system, is being commissioned at Palomar Observatory's 60-inch telescope. Here we discuss the instrument, scientific goals and results of initial on-sky operation.

  12. Robo-AO: An Autonomous Laser Adaptive Optics and Science System

    CERN Document Server

    Baranec, Christoph; Ramaprakash, A N; Law, Nicholas; Tendulkar, Shriharsh; Kulkarni, Shrinivas; Dekany, Richard; Bui, Khanh; Davis, Jack; Zolkower, Jeff; Fucik, Jason; Burse, Mahesh; Das, Hillol; Chordia, Pravin; Kasliwal, Mansi; Ofek, Eran; Morton, Timothy; Johnson, John

    2012-01-01

    Robo-AO, a fully autonomous, laser guide star adaptive optics and science system, is being commissioned at Palomar Observatory's 60-inch telescope. Here we discuss the instrument, scientific goals and results of initial on-sky operation.

  13. Additions to the marine algal flora of Curaçao, Netherlands Antilles

    NARCIS (Netherlands)

    Stegenga, H.; Vroman, M.

    1988-01-01

    Nine species of benthic marine algae are reported from the Caribbean island of Curaçao for the first time: Chlorophyta: Derbesia marina and Trichosolen longipedicellata; Phaeophyta: Ectocarpus rhodochortonoides, Feldmannia elachistaeformis, Hecatonema floridanum, Herponema tortugense and Sphacelaria

  14. Ciberespaço cubano: uma forma de resistência ao poder e à censura

    Directory of Open Access Journals (Sweden)

    Lia Hecker Luz

    2011-01-01

    Full Text Available A entrada do corpo e da vida nos cálculos do poder corresponde ao que Foucault chamou de biopolítica. Entretanto, o próprio Foucault intuiu que aquilo mesmo que o poder investia – a vida – era o que doravante ancoraria a resistência a ele. Assim, ao poder sobre a vida deveria responder a potência da vida, capaz de operar uma resistência possível ao sequestro da vida pelo poder. Apoiando-nos no pensamento de Lévy, levantamos a hipótese, a partir da análise do blog Generación Y, de que a blogosfera cubana é uma expressão micropolítica de resistência ao poder, de biopotência da multidão.

  15. A Conversation with his Honour Chief Judge Michael Rozenes AO County Court of Victoria

    Directory of Open Access Journals (Sweden)

    Michael Rozenes

    2014-10-01

    Full Text Available VULJ Editors and invited students interviewed the Chief Judge of the County Court of Victoria, His Honour Michael Rozenes AO QC, on Tuesday 19 August 2014 at the College of Law & Justice, Victoria University.

  16. Safety evaluation of pectin-derived acidic oligosaccharides (pAOS): Genotoxicity and sub-chronic studies

    NARCIS (Netherlands)

    Garthoff, J.A.; Heemskerk, S.; Hempenius, R.A.; Lina, B.A.R.; Krul, C.A.M.; Koeman, J.H.; Speijers, G.J.A.

    2010-01-01

    Pectin-derived acidic oligosaccharides (pAOS) are non-digestible carbohydrates to be used in infant formulae and medical nutrition. To support its safety, the genotoxic potential of pAOS was evaluated. pAOS was not mutagenic in the Ames test. Positive results were obtained in the chromosome aberrati

  17. Te Ao Kori as Expressive Movement in Aotearoa New Zealand Physical Education Teacher Education (PETE): A Narrative Account

    Science.gov (United States)

    Legge, Maureen

    2011-01-01

    A unique aspect of Aotearoa/New Zealand physical education is the inclusion of Maori culture in the form of te ao kori. Te ao kori translates to mean the world of movement and is represented by the interpretation of indigenous movement, games and pastimes. Participation in te ao kori means the sports-based normative frame of reference for physical…

  18. Attitude toward contraception and abortion among Curaçao women. Ineffective contraception due to limited sexual education?

    NARCIS (Netherlands)

    Brink, M.J.; Boersma, A.A.; Meyboom-deJong, B; Bruijn, de J.G.M.

    2011-01-01

    Background In Curaçao is a high incidence of unintended pregnancies and induced abortions. Most of the induced abortions in Curaçao are on request of the woman and performed by general practitioners. In Curaçao, induced abortion is strictly prohibited, but since 1999 there has been a policy of conni

  19. Percepção do consumidor frente aos riscos associados aos alimentos, sua segurança e rastreabilidade

    Directory of Open Access Journals (Sweden)

    Juliana Cunha de Andrade

    2013-09-01

    mostrou relevante nos três grupos, pois esta deve contribuir para o recolhimento mais eficiente de produtos, caso seja necessário. No entanto, sob a ótica do consumidor, a rastreabilidade leva ao aumento dos preços, embora aumente a confiança em relação à segurança dos alimentos. Muitas das preocupações dos consumidores refletem as informações publicadas em revistas e jornais, como o caso da crença de que hormônios são utilizados na cadeia da carne de frango. Indústria e varejistas devem incrementar a comunicação de informações científicas sobre a segurança dos alimentos, contribuindo, assim, na educação do consumidor.

  20. VizieR Online Data Catalog: Robo-AO Kepler planetary candidate survey. II. (Baranec+, 2016)

    Science.gov (United States)

    Baranec, C.; Ziegler, C.; Law, N. M.; Morton, T.; Riddle, R.; Atkinson, D.; Schonhut, J.; Crepp, J.

    2016-10-01

    We selected targets that we had not previously observed from the KOI Catalog based on the Q1-Q12 Kepler data (Rowe et al. 2015, Cat. J/ApJS/217/16). These targets were added to the Robo-AO intelligent observing queue and observed during the summer of 2013. We obtained high angular resolution images of 956 Kepler planet candidate host stars with the Robo-AO robotic laser AOs system over the course of 19 nights between 2013 July 21 and 2013 October 25, detailed in Table5. We also include 13 images from 2012 (2012 July 16-September 13) that required additional confirmation of the KOI position in the Robo-AO field of view. All the observations were performed in a queue-scheduled mode in combination with other science programs using the Robo-AO autonomous laser AO system mounted on the robotic 1.5m telescope at Palomar Observatory (exposure time: 90s; observation wavelengths: 600-950nm; FWHM resolution: 0.12''-0.15''; field of view: 44''*44''; pixel scale: 43.1mas/pix; detector format: 10242 pixels; targets observed/hour: 20). We obtained images of 50 KOIs with the NIRC2 instrument behind the Keck II AO system that were previously observed with Robo-AO and had evidence of a companion. Observations were conducted on 2013 June 25, 2013 August 24 and 25, 2014 August 17, and 2015 July 25 in the K, Ks, or Kp filters, and in the narrow mode of NIRC2 (9.952mas/pixel). (4 data files).

  1. Antecedents to the Perpetration of Domestic Violence in Curaçao

    OpenAIRE

    Wijk; Bruijn, de, CHMM Chris

    2015-01-01

    ABSTRACTPrevious international studies have found collectivism and low gender empowerment to contribute to higher domestic violence perpetration by males, compared to females. Little is known about gender differences in domestic violence perpetration prevalence in collectivist countries with high gender empowerment, for example Curaçao. Curaçao demonstrates gender similarity in committing domestic violence, resembling Western countries: 25–33 % have committed psychological domestic violence, ...

  2. Fatores associados ao baixo peso ao nascimento entre adolescentes no Sudeste do Brasil Factors associated with low birth weight among adolescents in the Brazil Southeast region

    Directory of Open Access Journals (Sweden)

    Fernanda Garanhani Castro Surita

    2011-10-01

    Full Text Available OBJETIVOS: Conhecer a prevalência e alguns fatores associados ao baixo peso ao nascer entre gestantes adolescentes. MÉTODOS: Estudo de corte transversal, realizado entre outubro de 1994 a dezembro de 2009, com os partos ocorridos em uma maternidade de Campinas, utilizando informações coletadas em uma Ficha Obstétrica Informatizada. Foram selecionados os casos de partos entre adolescentes e, posteriormente, separados em dois grupos com e sem baixo peso ao nascer. Foram calculados o risco relativo e o intervalo de confiança (IC de 95% para as variáveis independentes (fatores de risco, e calculou-se o teste do Χ2 para comparação dos resultados perinatais. Assumiu-se nível de significância de 5%. RESULTADOS: No período ocorreram 24.000 partos no Centro de Atenção à Saúde da Mulher (CAISM, com 2.404 em 2.357 adolescentes (10,02%, sendo a frequência de baixo peso ao nascer de 15,1%. A gestação neste grupo foi recorrente em 294 (8,2%. A idade menor que 15 anos, anemia, tabagismo e hipertensão não se associaram ao baixo peso ao nascer. O antecedente de aborto e a associação com lúpus eritematoso sistêmico elevaram o risco de baixo peso ao nascer. A necessidade de cesariana e o Apgar menor que sete também foram mais prevalentes entre as adolescentes com baixo peso ao nascer, e 85% das adolescentes realizaram menos de seis consultas durante o pré-natal. CONCLUSÕES: A prevalência de baixo peso ao nascer é maior entre as adolescentes do que na população geral. Também chamou a atenção o grande número de adolescentes com menos de seis consultas durante o pré-natal. O antecedente de aborto e a presença de lúpus eritematoso sistêmico foram fatores de risco para ocorrência de baixo peso ao nascer em gestantes adolescentes.PURPOSES: To determine the rate of low birth weight and some of the risk factors associated with this event among adolescents. METHODS: A cross-sectional study conducted between October 1994 and

  3. Decolorization of the AO24 azo dye and reduction of toxicity and genotoxicity in trickling biofilters.

    Science.gov (United States)

    Garzóón-Zúñga, Marco A; Sandoval-Villasana, Ana M; Moeller-Chávez, Gabriela E

    2011-02-01

    Acid Orange 24 (AO24) dye was degraded in a trickling biofilter packed with peat and wood chips and inoculated with biomass from a petrochemical industry wastewater system. Different operating strategies were tested; in the first stage, two biofilters were operated independently--one non-aerated biofilter (passive) and the other with aeration-subsequently, the systems were operated serially, and effluent from the non-aerated biofilter was fed to the biofilter with aeration. This treatment train was used to test three different filtration velocities--0.141, 0.282, and 0.423 m/d. The results show that, when operating the systems with a dye charge of 0.035 kg AO24 m2/d and treating the effluent in a single step, good removal efficiencies of AO24 (95 and 89%), COD (63 and 53%), and acute toxicity (63 and 78%) were obtained in both biofilters (with and without air), although mutagenic and potentially carcinogenic intermediary compounds were not removed, because genotoxicity exhibits values higher than 2.0 units for the mutation rate. When using the non-aerated biofilter/aerated biofilter treatment train, it is possible to treat a dye charge 3 times greater (0.106 kg AO24 m2/d) and efficiently remove 98% AO24, 76% COD, 100% acute toxicity, and 100% genotoxicity, which indicates that, with this biological system, an advanced degree of biotransformation and mineralization of the azo dye AO24 is achieved.

  4. MagAO: Status and on-sky performance of the Magellan adaptive optics system

    CERN Document Server

    Morzinski, Katie M; Males, Jared R; Kopon, Derek; Hinz, Phil M; Esposito, Simone; Riccardi, Armando; Puglisi, Alfio; Pinna, Enrico; Briguglio, Runa; Xompero, Marco; Quirós-Pacheco, Fernando; Bailey, Vanessa; Follette, Katherine B; Rodigas, T J; Wu, Ya-Lin; Arcidiacono, Carmelo; Argomedo, Javier; Busoni, Lorenzo; Hare, Tyson; Uomoto, Alan; Weinberger, Alycia

    2014-01-01

    MagAO is the new adaptive optics system with visible-light and infrared science cameras, located on the 6.5-m Magellan "Clay" telescope at Las Campanas Observatory, Chile. The instrument locks on natural guide stars (NGS) from 0$^\\mathrm{th}$ to 16$^\\mathrm{th}$ $R$-band magnitude, measures turbulence with a modulating pyramid wavefront sensor binnable from 28x28 to 7x7 subapertures, and uses a 585-actuator adaptive secondary mirror (ASM) to provide flat wavefronts to the two science cameras. MagAO is a mutated clone of the similar AO systems at the Large Binocular Telescope (LBT) at Mt. Graham, Arizona. The high-level AO loop controls up to 378 modes and operates at frame rates up to 1000 Hz. The instrument has two science cameras: VisAO operating from 0.5-1 $\\mu$m and Clio2 operating from 1-5 $\\mu$m. MagAO was installed in 2012 and successfully completed two commissioning runs in 2012-2013. In April 2014 we had our first science run that was open to the general Magellan community. Observers from Arizona, Ca...

  5. Aspectos ecológicos da tripanossomose americana: II - distribuição e dispersão local de triatomíneos em ecótopos naturais e artificiais Ecological aspects of South American tripanosomiasis: II - local distribution and dispersion of triatomids in natural and artificial ecotopes

    Directory of Open Access Journals (Sweden)

    Oswaldo Paulo Forattini

    1971-12-01

    Full Text Available São apresentados os resultados de investigação destinada a estabelecer o quadro de distribuição de Triatoma sordida e Rhodnius neglectus em áreas com ecótopos naturais e artificiais. Essa distribuição revelou-se do tipo agrupado ou contagiante, dirigida uniformemente para casas e anexos por parte do primeiro, e para palmeiras por parte do segundo. Em áreas de vegetação mais densa, do tipo cerrado, a densidade é praticamente nula ou muito baixa, admitindo-se que isso deva correr por conta da competição. As modificações introduzidas pelo homem tendem a anular êsse fator, além de, as suas habitações, se transformarem em ecótopos que atraem a população de T. sordida. Êsse fato, aliado à eliminação local do T. infestans, parece explicar a tendência invasiva daquele triatomíneo. Evidenciou-se também a persistência da infecção pelo Trypanosoma cruzi em triatomíneos e vertebrados domiciliados, o que representa potencial para o incremento da transmissão.A local distribution pattern of Triatoma sordida and Rhodnius neglectus is described after an intensive investigation on natural and artificial ecotopes. The work was carried in a region at north of the State of São Paulo, Brazil. Measures of dispersion pattern were made by the Morisita's indices showing contagious distributions to both bugs. The T. sordida population was clumped at houses and surrounding dwellings, while R. neglectus presented the same aspect but relater to palm trees. The first bug showed ubiquity patter including palms, dry trees and fences. These last two situations are not favourable for obtaining blood meals, who are much more easy obtained at human dwellings. By other side, the investigations at areas with more dense vegetation like bush, resulted in very few T. sordida and none R. neglectus, probably owing of other populations competition as predators reduvids bugs, found there with great density. So it seems logic remove that competition and

  6. Aspectos ecológicos da tripanossomíase americana: XXI - Comportamento de espécies triatomíneas silvestres na reinfestação do intra e peridomicílio Ecological aspects of South American trypanosomiasis: XXI - Sylvatic triatominae behaviour related to the domiciliary and peridomiciliary environments

    Directory of Open Access Journals (Sweden)

    Oswaldo Paulo Forattini

    1984-06-01

    Full Text Available Apresentam-se os resultados obtidos com a observação da presença triatomínea em três localidades, uma delas caracterizada por estar submetida a vigilância anual ininterrupta, e duas por terem sido expurgadas respectivamente seis e um ano e meio antes. As duas primeiras situam-se em áreas de endemicidade de Panstrongylus megistus e a terceira em região endêmica para Triatoma sordida. Em todas foram levadas a efeito inspeções bimestrais destinadas a detectar a presença de triatomíneos no intra e no peridomicílio. Pôde-se confirmar o aspecto lento da domiciliação de P. megistus, em aparente contraste com o T. sordida que desenvolve intensas colônias peridomiciliares. Pelo menos em relação a esta última espécie, não parece haver diferença atrativa por parte do intra e do peridomicílio. A reinfestação do P. megistus acha-se associada freqüente com a presença de infecção natural. A reintrodução de Triatoma infestans associa-se à mobilidade da população humana. A colonização peridomiciliar de T. sordida permitiu o estudo do desenvolvimento dessas colônias, obtendo-se marcante paralelismo com os dados conseguidos em galinheiros experimentais (GE e objeto de publicações anteriores. A presença de Rhodnius neglectus nas três áreas, embora discreta, chama a atenção para seu possível potencial de domiciliação. Com este artigo encerra-se a série "Aspectos ecológicos de tripanossomíase americana".The results of observations on triatominae bugs in three localities, one of them under to active annual regular surveillance and the other two which had undergone house treatment six months and one and half years before, respectively, are reported. Two of these localities were situated in the dispersal center region of Panstrongylus megistus, and the third in the dispersal center region of Triatoma sordida. At two-monthly periods each area was submitted to dwelling inspection in the search for triatominae bugs in

  7. Excito-repellency effect of deltamethrin on triatomines under laboratory conditions

    Directory of Open Access Journals (Sweden)

    Diotaiuti Liléia

    2000-01-01

    Full Text Available This work demonstrates that deltamethrin in low doses produces an excito-repellency effect on triatomines, as already observed for mosquitoes. A wooden box covered with a cloth impregnated with deltamethrin at doses of 2.5 and 5mg ai/m² was utilized for the experiment. The triatomine species studied were Triatoma infestans, Panstrongylus megistus, Rhodnius neglectus and Triatoma sordida. Adults were released in one of the sides of the box and their position was noted in subsequent periods. The observations were realized on the day the cloth was impregnated and subsequently repeated at 30 and 60 days for T. sordida; on day 120, the remaining species were included. Insect mortality and attempts at flight from the box were also observed. Excito-repellency was evident for all species and doses up to day 120. The only species that attempted to fly was P. megistus. The excito-repellency effect may be considered as an additional advantage to the insecticide power, as it should be able to prevent the installation of new colonies by females that fly into the homes, and at the moment of the spraying, it should promote the flushing out of triatomines from the wall crevices and from other shelters.

  8. Instrumento mensurador de adesao para hipertensos: contribuicao da Teoria da Resposta ao Item

    Directory of Open Access Journals (Sweden)

    Malvina Thais Pacheco Rodrigues

    2013-06-01

    Full Text Available OBJETIVO: Analisar instrumento de medição da adesão ao tratamento da hipertensão por meio da "Teoria da Resposta ao Item". MÉTODOS: Estudo analítico com 406 hipertensos com complicações associadas, atendidos na atenção básica em Fortaleza, CE, 2011, pela "Teoria da Resposta ao Item". As etapas de execução foram: teste de dimensionalidade, calibração dos itens; tratamento dos dados e construção da escala, analisadas com base no modelo de resposta gradual. Estudo da dimensionalidade do instrumento foi feito pela análise da matriz de correlação policórica e análise fatorial de informação completa. Utilizou-se o software Multilog para calibração dos itens e estimação dos escores. RESULTADOS: Os itens referentes ao tratamento medicamentoso foram os mais diretamente relacionados à adesão, enquanto aqueles referentes ao tratamento não medicamentoso precisam ser reformulados, pois possuíram menor quantidade de informação psicométrica e baixa discriminação. A independência dos itens, o reduzido número de níveis da escala e as baixas variâncias explicadas no ajuste dos modelos mostraram as principais fragilidades do instrumento analisado. A "Teoria da Resposta ao Item" mostrou-se relevante para análise, pois avaliou o respondente quanto à adesão ao tratamento da hipertensão, ao nível de dificuldade dos itens e à sua capacidade de discriminação entre indivíduos com diferentes níveis de adesão, o que gerou maior quantidade de informação. CONCLUSÕES: O instrumento analisado é limitado para medir a adesão ao tratamento da hipertensão, mediante análise pela "Teoria da Resposta ao Item", e necessita de ajustes. A adequada formulação dos itens é importante para medir precisamente o traço latente desejado.

  9. Photometric Properties for Selected Algol-type Binaries. II. AO Serpentis and V338 Herculis

    Science.gov (United States)

    Yang, Y.-G.; Hu, S.-M.; Guo, D.-F.; Wei, J.-Y.; Dai, H.-F.

    2010-04-01

    We present the first multiband photometry for the semidetached eclipsing binary AO Serpentis, observed on seven nights between 2009 April and July at the Weihai Observatory of Shandong University. By using the 2003 version of the Wilson-Devinney code, the photometric solutions of AO Ser and a similar object V338 Her were (re)deduced. The spectral types and orbital periods are A2 and P = 0.8793 days for AO Ser, F1V and P = 1.3057 days for V338 Her. The results reveal that two binaries are low mass ratio systems, whose secondary components fill their Roche lobes. The fill-out factors of the primary components are f = 58.6% for AO Ser and f = 54.2% for V338 Her, respectively. From the O - C curves of AO Ser and V338 Her, it is discovered that secular period changes with cyclic variations exist. The periods and semiamplitudes are 17.32(±0.01) yr and 0.0051(±0.0001) days for AO Ser, 29.07(±0.04) yr and 0.0116(±0.0015) days for V338 Her, respectively. This kind of cyclic oscillation may be attributed to either the light-time effect via an assumed third body or perhaps cyclic magnetic activity on the secondary component. For AO Ser, the long-term period decreases at a rate of dP/dt = -5.35(±0.03) × 10-7 days yr-1, which may be caused by mass and angular momentum loss from the system. Considering the period decreasing, the fill-out factor of the primary for AO Ser will increase and it will finally fill its Roche lobe. Meanwhile, the secular period increase rate for V338 Her is dP/dt = +1.44(±0.24) × 10-7 days yr-1, indicating that mass transfers from the less massive component to the more massive component. This will also cause the fill-out factor of the primary to increase. When the primaries fill their Roche lobes, AO Ser and V338 Her may evolve into contact stars, as predicted by the theory of thermal relaxation oscillations.

  10. Shearing of petroleum A/O emulsions during line sampling; Cisalhamento de emulsoes de petroleo do tipo A/O durante amostragem em linha

    Energy Technology Data Exchange (ETDEWEB)

    Ramalho, Joao Batista Vianey da Silva [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES). Tecnologia de Escoamento e Elevacao Artificial]. E-mail: jramalho@cenpes.petrobras.com.br

    2002-03-01

    Several studies have been performed to evaluate water-oil separation in petroleum A/O emulsions, employing samples that were collected directly from the field by direct depressurization of the fluid contained inside the drainage line. In these studies, the development of de-emulsifier formulations and the evaluation of the performance of these products in a laboratory setting are highlighted. Often, differences are observed in the performance between the curves obtained in the laboratory and in the field. The distribution of the size of the drops in A/O type emulsions that were collected from the field under atmospheric and pressurized conditions was quantified by using laser diffraction. A significant decrease in the size of the drops was observed when collection was performed with direct depressurization of the fluid in the sample outlet. (author)

  11. Cuidados ao recém nascido em fototerapia: o conhecimento da equipe de enfermagem

    Directory of Open Access Journals (Sweden)

    Nathália Silva Gomes

    2010-06-01

    Full Text Available A icterícia é uma das alterações mais frequentes tanto em recém-nascidos a termo quanto em prematuros. A fototerapia é otratamento mais utilizado nestes casos, porém este tratamento pode ocasionar algumas alterações no bebê, as quais devem ser prevenidas e detectadas precocemente pela equipe de enfermagem. O objetivo do estudo foi identificar o conhecimento da equipe de enfermagem acerca dos cuidados ao recém-nascido e complicações relacionadas a fototerapia. Trata-se de um estudo prospectivo e descritivo realizado com a equipe de enfermagem dos setores de Serviço de enfermagem da Unidade Neonatológica, Alojamento Conjunto e CTI Neonatal de um Hospital Universitário. As temáticas da entrevista abordaram sobre: quando é iniciada a fototerapia no RN, quem realiza a avaliação da evolução da icterícia, quais os possíveis efeitoscolaterais no RN e quem os avalia, e sobre os cuidados realizados ao RN em fototerapia e aos equipamentos utilizados. Percebemos que o melhor conhecimento da equipe de enfermagem sobre os cuidados relacionados ao recém-nascido em fototerapia e aos aparelhos utilizados propicia maior qualidade na assistência, resultados mais rápidos e eficazes e reconhecimento do trabalho da enfermagem no cuidado.

  12. Adesão de pessoas com diabetes mellitus tipo 2 ao tratamento medicamentoso

    Directory of Open Access Journals (Sweden)

    Lilian Cristiane Gomes-Villas Boas

    2014-04-01

    Full Text Available Estudo seccional de abordagem quantitativa que objetivou avaliar a adesão ao tratamento medicamentoso entre pessoas com diabetes mellitus, e sua relação com variáveis clínicas, de tratamento e controle metabólico. A amostra constituiu-se de 162 pessoas com diabetes mellitus tipo 2 em seguimento ambulatorial. Para a coleta dos dados, foram utilizadas a Medida de Adesão aos Tratamentos e a consulta aos prontuários dos participantes. Obteve-se elevada adesão ao tratamento medicamentoso. Para um p<0,05, obteve-se correlação inversa com a pressão arterial diastólica e correlação direta com a frequência diária de administração de insulina e de antidiabéticos orais. Não houve correlações estatisticamente significantes entre adesão e variáveis de controle metabólico. Os resultados divergem da literatura no que se refere à taxa de adesão ao tratamento medicamentoso em doenças crônicas, bem como na correlação entre adesão e complexidade do regime medicamentoso, o que aponta para a necessidade de mais estudos sobre essa temática.

  13. Morfologia e Crescimento dos 6 aos 10 anos de idade em Viana do Castelo, Portugal

    Directory of Open Access Journals (Sweden)

    Luis Paulo Rodrigues

    2008-09-01

    Full Text Available Ao longo de quatro anos, o Estudo Morfofuncional da Criança Vianense observou 1911 crianças entre os 6e os 10 anos de idade, resultando em 4064 observações individuais(2054 de raparigas, 2006 de rapazes.Os resultados encontrados nos indicadores morfológicos simples(altura, peso, pregas adiposas, diâmetros ósseos e perímetros muscularese no somatótipo, são descritos normativamente (valores percentílicose comparados com outros estudos nacionais e internacionais. As crianças vianenses demonstraram possuir uma estatura média ligeiramente superior às reportadas nos estudos portugueses e um ritmo de crescimento diferente das norte-americanas; peso semelhante ao das congéneres nacionais mas inferior ao das EUA; valores de pregas adiposas geralmente inferiores aos encontrados em Portugal e EUA; perímetros musculares e diâmetros ósseos semelhantes aos seus pares portugueses; e uma tendência para o aumento, com a idade, do ectomorfismo nos rapazes e do endomorfismo em ambos os sexos. Este panorama parece indicar que as crianças vianenses apresentam características de aptidão morfologia que estão longe de espelhar as preocupações internacionais nesta matéria.

  14. Práticas educativas maternas e paternas aos 24 e aos 72 meses de vida da criança

    Directory of Open Access Journals (Sweden)

    Angela Helena Marin

    Full Text Available Este estudo investigou as práticas educativas indutivas, coercitivas e de não interferência maternas e paternas aos 24 e 72 meses de vida da criança. Participaram 24 mães e pais de um único filho/a, que responderam uma entrevista para avaliação dessas práticas. Análises estatísticas revelaram que as mães foram significativamente mais indutivas que os pais aos 24 meses, mas aos 72 meses não houve diferenças. Enfatiza-se que as mães tendem a conversar mais com seus filhos/as, expressar sentimentos, opiniões, estabelecer limites e elogiar comportamentos adequados. As mães também apresentaram médias mais elevadas no total de práticas relatadas, o que pode ser explicado pelo papel predominante que ainda exercem na socialização infantil, embora os pais venham participando mais da educação dos filhos/as.

  15. FIELD EVALUATION OF THE LIGNIN-DEGRADING FUNGUS PHANEROCHAETE SORDIDA TO TREAT CREOSOTE-CONTAMINATED SOIL

    Science.gov (United States)

    A field study to determine the ability of selected lignin-degrading fungi to remediate soil contaminated with creosote was performed at a wood-treating facility in south central Mississippi in the autumn of 1991. The effects of solid-phase bioremediation with Phanerochaete sordid...

  16. Heterologous expression of a lectin from Pleurocybella porrigens (PPL) in Phanerochaete sordida YK-624

    OpenAIRE

    Suzuki, Tomohiro; Dohra, Hideo; Omae, Saori; Takeshima, Yoshino; Choi, Jae-Hoon; Hirai, Hirofumi; Kawagishi, Hirokazu

    2014-01-01

    NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Microbiological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Microbiological Methods, VOL100, May 2014. DOI...

  17. Up-regulated expression of AOS-LOXa and increased eicosanoid synthesis in response to coral wounding.

    Directory of Open Access Journals (Sweden)

    Helike Lõhelaid

    Full Text Available In octocorals, a catalase-like allene oxide synthase (AOS and an 8R-lipoxygenase (LOX gene are fused together encoding for a single AOS-LOX fusion protein. Although the AOS-LOX pathway is central to the arachidonate metabolism in corals, its biological function in coral homeostasis is unclear. Using an acute incision wound model in the soft coral Capnella imbricata, we here test whether LOX pathway, similar to its role in plants, can contribute to the coral damage response and regeneration. Analysis of metabolites formed from exogenous arachidonate before and after fixed time intervals following wounding indicated a significant increase in AOS-LOX activity in response to mechanical injury. Two AOS-LOX isoforms, AOS-LOXa and AOS-LOXb, were cloned and expressed in bacterial expression system as active fusion proteins. Transcription levels of corresponding genes were measured in normal and stressed coral by qPCR. After wounding, AOS-LOXa was markedly up-regulated in both, the tissue adjacent to the incision and distal parts of a coral colony (with the maximum reached at 1 h and 6 h post wounding, respectively, while AOS-LOXb was stable. According to mRNA expression analysis, combined with detection of eicosanoid product formation for the first time, the AOS-LOX was identified as an early stress response gene which is induced by mechanical injury in coral.

  18. O Ocaso da Utopia Telecomunicacional: regular para quem no tocante ao Direito Fundamental à Privacidade?

    Directory of Open Access Journals (Sweden)

    Conselho Editorial

    2014-05-01

    Full Text Available O presente estudo figura como introdução à Revista de Direito, Estado e Telecomunicações do Grupo de Estudos em Direito das Telecomunicações da Universidade de Brasília, abordando sinteticamente a estrutura, conteúdo e política editorial da revista. Em acréscimo, o texto analisa os principais acontecimentos do setor no Brasil, bem como normas e julgados relativos ao ano de 2013, para registro das principais discussões político-jurídicas do setor de telecomunicações brasileiro referentes ao ano anterior ao da publicação.

  19. ELT instrumentation for seeing-limited and AO-corrected observations: A comparison

    CERN Document Server

    Cunningham, Colin; Monnet, Guy; Louarn, Miska Le

    2008-01-01

    The next generation of large ground-based optical and infrared telescopes will provide new challenges for designers of astronomical instrumentation. The varied science cases for these extremely large telescopes (ELTs) require a large range of angular resolutions, from near diffraction-limited performance via correction of atmospheric turbulence using adaptive optics (AO), to seeing-limited observations. Moreover, the scientific output of the telescopes must also be optimized with the consideration that, with current technology, AO is relatively ineffective at visible wavelengths, and that atmospheric conditions will often preclude high-performance AO. This paper explores some of the issues that arise when designing ELT instrumentation that operates across a range of angular resolutions and wavelengths. We show that instruments designed for seeing-limited or seeing-enhanced observations have particular challenges in terms of size and mass, while diffraction-limited instruments are not as straightforward as mig...

  20. A Arqueologia do cuidado: da prática ao discurso

    Directory of Open Access Journals (Sweden)

    Sidi Askofare

    2006-06-01

    Full Text Available A noção de cuidado, assimilada há muito tempo ao tratamento e, então, ao conceito de terapêutica, retorna, logo depois, nos estudos, pesquisas e reflexões nas ciências humanas. Mas, daí em diante, está emancipado de suas aderências e conotações médicas por serem demasiadamente redutoras. O presente artigo propõe uma arqueologia (no sentido foucaultiano do cuidado &– de suas origens animais às suas formas culturalmente e historicamente definidas &– e põe em evidência a subversão que produz no campo do cuidado &– das práticas aos discursos &– a emergência e a dominação do discurso da ciência.

  1. Obtenção de plantas de feijão-caupi resistentes ao Cowpea severe mosaic virus e ao Cowpea aphid-borne mosaic virus

    Directory of Open Access Journals (Sweden)

    Gislanne Brito Barros

    2013-06-01

    Full Text Available Dentre os vírus que infectam o feijão-caupi (Vigna unguiculata L. Walp. destacam-se, respectivamente, pela severidade e ampla ocorrência o Cowpea severe mosaic virus (CPSMV e o Cowpea aphid-borne mosaic virus (CABMV. Portanto, objetivaram-se, no presente trabalho, obter e avaliar plantas de feijão-caupi com resistência ao CPSMV e ao CABMV, visando ao desenvolvimento de cultivares essencialmente derivadas e novas cultivares. Realizaram-se oito cruzamentos seguidos de retrocruzamentos, utilizando a linhagem TE 97-309G-9 e a cultivar Patativa como genitores resistentes, e as cultivares BR3-Tracuateua, BRS-Urubuquara, BRS-Novaera, BRS-Guariba e Pretinho como genitores suscetíveis. As gerações F2 e F2RC1 foram desafiadas quanto à resistência por meio de inoculação mecânica com isolados do CPSMV e do CABMV. Nas gerações F2RC1, além da resistência foram avaliados os caracteres: número de dias para o início da floração, comprimento das vagens, número de grãos. vagem-1, peso de cem grãos e produção de grãos.planta-1. Todos os indivíduos F2 e F2RC1 foram analisados pelo teste χ² e se ajustaram à frequência esperada de 15 plantas suscetíveis 1 planta resistente a ambos os vírus. As médias das plantas F2RC1 resistentes, de cada retrocruzamento, foram comparadas com a média do seu respectivo genitor recorrente pelo teste 't' e as médias dos retrocruzamentos foram comparadas pelo teste de Scott-Knott. Foi detectada variabilidade genética entre os retrocruzamentos para todos os caracteres. Todos os retrocruzamentos foram considerados promissores para produção de cultivares essencialmente derivadas resistentes ao CPSMV e ao CABMV e as plantas selecionadas possuem características que possibilitam a seleção de linhagens com grãos de bom padrão comercial e altamente produtivas.

  2. Characterization of phosphorus removal bacteria in (AO)2 SBR system by using different electron acceptors

    Institute of Scientific and Technical Information of China (English)

    JIANG Yi-feng; WANG Lin; YU Ying; WANG Bao-zhen; LIU Shuo; SHEN Zheng

    2007-01-01

    Characteristics of phosphorus removal bacteria were investigated by using three different types of electron acceptors, as well as the positive role of nitrite in phosphorus removal process. An (AO)2 SBR (anaerobic-aerobic-anoxic-aerobic sequencing batch reactor) was thereby employed to enrich denitrifying phosphorus removal bacteria for simultaneously removing phosphorus and nitrogen via anoxic phosphorus uptake. Ammonium oxidation was controlled at the first phase of the nitrification process. Nitrite-inhibition batch tests illustrated that nitrite was not an inhibitor to phosphorus uptake process, but served as an alternative electron acceptor to nitrate and oxygen if the concentration was under the inhibition level of 40mg NO2 - N · L- 1. It implied that in addition to the two well-accepted groups of phosphorus removal bacterium ( one can only utilize oxygen as electron acceptor, P1, while the other can use both oxygen and nitrate as electron acceptor, P2 ), a new group of phosphorus removal bacterium P3, which could use oxygen, nitrate and nitrite as electron acceptor to take up phosphorus were identified in the test system. To understand (AO)2 SBR sludge better, the relative population of the different bacteria in this system, plus another A/O SBR sludge (seed sludge) were respectively estimated by the phosphorus uptake batch tests with either oxygen or nitrate or nitrite as electron acceptor. The results demonstrated that phosphorus removal capability of (AO)2 SBR sludge had a little degradation after A/O sludge was cultivated in the (AO)2 mode over a long period of time. However, denitrifying phosphorus removal bacteria ( P2 and P3 ) was significantly enriched showed by the relative population of the three types of bacteria,which implied that energy for aeration and COD consumption could be reduced in theory.

  3. Genes regulated by AoXlnR, the xylanolytic and cellulolytic transcriptional regulator, in Aspergillus oryzae.

    Science.gov (United States)

    Noguchi, Yuji; Sano, Motoaki; Kanamaru, Kyoko; Ko, Taro; Takeuchi, Michio; Kato, Masashi; Kobayashi, Tetsuo

    2009-11-01

    XlnR is a Zn(II)2Cys6 transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Overexpression of the aoxlnR gene in Aspergillus oryzae (A. oryzae xlnR gene) resulted in elevated xylanolytic and cellulolytic activities in the culture supernatant, in which nearly 40 secreted proteins were detected by two-dimensional electrophoresis. DNA microarray analysis to identify the transcriptional targets of AoXlnR led to the identification of 75 genes that showed more than fivefold increase in their expression in the AoXlnR overproducer than in the disruptant. Of these, 32 genes were predicted to encode a glycoside hydrolase, highlighting the biotechnological importance of AoXlnR in biomass degradation. The 75 genes included the genes previously identified as AoXlnR targets (xynF1, xynF3, xynG2, xylA, celA, celB, celC, and celD). Thirty-six genes were predicted to be extracellular, which was consistent with the number of proteins secreted, and 61 genes possessed putative XlnR-binding sites (5'-GGCTAA-3', 5'-GGCTAG-3', and 5'-GGCTGA-3') in their promoter regions. Functional annotation of the genes revealed that AoXlnR regulated the expression of hydrolytic genes for degradation of beta-1,4-xylan, arabinoxylan, cellulose, and xyloglucan and of catabolic genes for the conversion of D-xylose to xylulose-5-phosphate. In addition, genes encoding glucose-6-phosphate 1-dehydrogenase and L-arabinitol-4- dehydrogenase involved in D-glucose and L-arabinose catabolism also appeared to be targets of AoXlnR.

  4. Adesão ao Tratamento em Gestação de Alto Risco

    Directory of Open Access Journals (Sweden)

    Fabíola Langaro

    Full Text Available No Brasil, aproximadamente 15% das gestações são de alto risco, sendo os diagnósticos de diabetes gestacional e hipertensão as causas mais frequentes dessa condição. Para a efetividade dos tratamentos indicados nesses casos, bem como alcance de desfechos favoráveis, é essencial a adesão da paciente às recomendações fornecidas pelas equipes de saúde, o que torna fundamental a avaliação desse indicador. A pesquisa apresentada avaliou a adesão de participantes de um programa de atendimento interdisciplinar a gestantes de alto risco desenvolvido em um hospital geral privado por meio de três instrumentos: Questionário Morisky-Green de adesão ao uso de medicação, Questionário de Adesão ao Tratamento e Pergunta descritiva de avaliação qualitativa. Durante oito meses, 83 gestantes responderam aos questionários, que possibilitaram identificar níveis de adesão ao longo do tempo de participação no programa, situações de não adesão mais frequentes, bem como variáveis que interferem nas medidas de adesão ao uso de medicação e ao tratamento global. Considera-se que os índices levantados são passíveis de uso como indicadores clínicos e gerenciais, possibilitando a comprovação da efetividade e melhoria constante das rotinas de saúde. Tais mecanismos são úteis para definição de protocolos, discussão de casos clínicos e mesmo para feedbackàs próprias pacientes.

  5. Novel algorithm implementations in DARC: the Durham AO real-time controller

    Science.gov (United States)

    Basden, Alastair; Bitenc, Urban; Jenkins, David

    2016-07-01

    The Durham AO Real-time Controller has been used on-sky with the CANARY AO demonstrator instrument since 2010, and is also used to provide control for several AO test-benches, including DRAGON. Over this period, many new real-time algorithms have been developed, implemented and demonstrated, leading to performance improvements for CANARY. Additionally, the computational performance of this real-time system has continued to improve. Here, we provide details about recent updates and changes made to DARC, and the relevance of these updates, including new algorithms, to forthcoming AO systems. We present the computational performance of DARC when used on different hardware platforms, including hardware accelerators, and determine the relevance and potential for ELT scale systems. Recent updates to DARC have included algorithms to handle elongated laser guide star images, including correlation wavefront sensing, with options to automatically update references during AO loop operation. Additionally, sub-aperture masking options have been developed to increase signal to noise ratio when operating with non-symmetrical wavefront sensor images. The development of end-user tools has progressed with new options for configuration and control of the system. New wavefront sensor camera models and DM models have been integrated with the system, increasing the number of possible hardware configurations available, and a fully open-source AO system is now a reality, including drivers necessary for commercial cameras and DMs. The computational performance of DARC makes it suitable for ELT scale systems when implemented on suitable hardware. We present tests made on different hardware platforms, along with the strategies taken to optimise DARC for these systems.

  6. Deep-water sponges (Porifera) from Bonaire and Klein Curaçao, Southern Caribbean.

    Science.gov (United States)

    Van Soest, Rob W M; Meesters, Erik H W G; Becking, Leontine E

    2014-10-29

    Four submersible dives off the coast of Bonaire (Caribbean Netherlands) and Klein Curaçao (Curaçao) to depths of 99.5-242 m, covering lower mesophotic and upper dysphotic zones, yielded 52 sponge specimens belonging to 31 species. Among these we identified 13 species as new to science. These are Plakinastrella stinapa n. sp., Pachastrella pacoi n. sp., Characella pachastrelloides n. sp., Geodia curacaoensis n. sp., Caminus carmabi n. sp., Discodermia adhaerens n. sp., Clathria (Microciona) acarnoides n. sp., Antho (Acarnia) pellita n. sp., Parahigginsia strongylifera n. sp., Calyx magnoculata n. sp., Neopetrosia dutchi n. sp., Neopetrosia ovata n. sp. and Neopetrosia eurystomata n. sp. We also report an euretid hexactinellid, which belongs to the rare genus Verrucocoeloidea, recently described (2014) as V. liberatorii Reiswig & Dohrmann. The remaining 18 already known species are all illustrated by photos of the habit, either in situ or 'on deck', but only briefly characterized in an annotated table to confirm their occurrence in the Southern Caribbean. The habitat investigated-steep limestone rocks, likely representing Pleistocene fossil reefs--is similar to deep-water fossil reefs at Barbados of which the sponges were sampled and studied by Van Soest and Stentoft (1988). A comparison is made between the two localities, showing a high degree of similarity in sponge composition: 53% of the present Bonaire-Klein Curaçao species were also retrieved at Barbados. At the level of higher taxa (genera, families) Bonaire-Klein Curaçao shared approximately 80% of its lower mesophotic and upper dysphotic sponge fauna with Barbados, despite a distance between them of 1000 km, indicating high faunal homogeneity. We also preliminarily compared the shallow-water (euphotic) sponge fauna of Curaçao with the combined data available for the Barbados, Bonaire and Klein Curaçao mesophotic and upper dysphotic sponges, which resulted in the conclusion that the two faunas show only

  7. Imunidade celular em caninos neonatos - do nascimento ao 45° dia de idade

    Directory of Open Access Journals (Sweden)

    R.P. Klein

    2014-06-01

    Full Text Available O objetivo do presente trabalho foi acompanhar o desenvolvimento imunológico dos neonatos caninos, a fim de avaliar a imunidade celular pela análise dos leucócitos e linfócitos totais e das subpopulações de linfócitos T (CD4+ e CD8+ pela técnica de citometria de fluxo. Foram utilizados 30 cães neonatos de ambos os sexos, sem raça definida, aos três, 10, 17, 24, 31, 38 e 45 dias de idade. A contagem de leucócitos totais aos 45 dias (11.639±3.574 foi significativamente maior que no terceiro dia de idade (8.740±1.812 (P<0,05; não houve diferença entre a contagem total de linfócitos aos 45 dias em relação ao terceiro dia de idade. Quanto às subpopulações de LT CD4+ e LT CD8+, os percentuais de LT CD4+, aos três dias de idade (24,9±16,8%, foram inferiores quando comparados à média entre o 10°, o 24° e o 31°dia (35,5%, e os de CD8+, ao terceiro dia, menores em relação às médias do 10° e do 31° dia de idade. Pode-se concluir que as subpopulações de LT CD4+ e CD8+ sofrem oscilações durante o desenvolvimento pós-natal, sendo estas crescentes em relação aos níveis obtidos aos três dias de idade. A relação CD4+:CD8+ mostrou superioridade para o primeiro tipo celular, sendo que a maior relação entre CD4+ e CD8+ ocorreu no terceiro dia de idade. Com base nos resultados obtidos neste estudo, notaram-se as diferenças semanais nas populações linfocitárias, o que demonstra a dinâmica dessas células durante o período neonatal.

  8. O panorama das Drogas no México: da margem da sociedade ao centro da cultura

    OpenAIRE

    Diana Palaversich

    2013-01-01

    O artigo destaca o percurso das manifestações culturais ligadas ao tráfico de drogas no México, de um lugar marginal, de subcultura, na sociedade mexicana das décadas de 1970 e 1980, expressas principalmente através dos narcocorridos e do narcocine, para um lugar no mainstream cultural mexicano, desde o início do século 21, com destaque especial para a narcoliteratura. O aumento da violência, provocado pela guerra ao narcotráfico, produz uma proliferação da temática nos meios de comunicação e...

  9. Ressalto no canto posteromedial do joelho relacionado ao músculo sartório

    Directory of Open Access Journals (Sweden)

    Marcello Henrique Nogueira-Barbosa

    2011-06-01

    Full Text Available As causas da síndrome de ressalto no joelho relatadas com maior frequência são relacionadas a anormalidades meniscais, ao tendão do músculo bíceps femoral e aos tendões grácil e semitendíneo. Neste trabalho é descrito um caso de ressalto no canto posteromedial relacionado à junção miotendínea do músculo sartório em um paciente do sexo masculino, de 58 anos de idade, com hiperextensão do joelho.

  10. Resistência ao Consumo em um Circuito Urbano de Parkour

    Directory of Open Access Journals (Sweden)

    Tatiana Maria Bernardo da Silva

    2011-08-01

    Full Text Available A partir de uma pesquisa alinhada ao método etnográfico, o presente trabalho tem por objetivo investigar os valores e as práticas de resistência ao consumo adotadas pelos membros de um circuito urbano de parkour no Rio de Janeiro. Confiando em uma abordagem indutiva e interpretativa amparada por um estudo empírico de base qualitativa, foram exploradas quatro categorias de análise: (1 a resistência como valor essencial; (2 um percurso de doação; (3 o consumo aprisiona; e (4 mobilização social e resistência.

  11. Sistemas de vigilância das resistências aos antibióticos

    OpenAIRE

    Sousa, Patrícia Sofia Tomé de

    2016-01-01

    Dissertação de Mestrado, Ciências Farmacêuticas, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2015 A resistência aos antimicrobianos é um problema de saúde pública grave a nível mundial, afetando países desenvolvidos e em desenvolvimento e conduz a falhas terapêuticas, sofrimento prolongado do indivíduo e aumento dos custos dos cuidados de saúde, constituindo assim um encargo para a sociedade. Os sistemas de vigilância das resistências aos antimicrobianos são fundamentai...

  12. Comportamentos de agentes educativos como parte de contingências de ensino de comportamentos ao estudar

    Directory of Open Access Journals (Sweden)

    Ana Lucia Cortegoso

    Full Text Available Observando sessões de estudo de crianças atendidas em agência educacional, foram identificados comportamentos de agentes educativos como “comentar trabalhos realizados”, “identificar situações para intervir”, “apresentar conseqüências para estudar” e “intervir diante de comportamentos concorrentes ao estudar”, e propriedades desses comportamentos, como freqüência, duração, latência, forma e distribuição temporal, componentes de contingências relacionadas à aquisição e manutenção de comportamentos ao estudar.

  13. Adesão ao tratamento da tuberculose: Aspectos da vulnerabilidade individual e social

    OpenAIRE

    2015-01-01

    Elementos de vulnerabilidade individual e social podem interferir na adesão ao tratamento da tuberculose (TB), contextualizados no ambiente o qual o doente está inserido. Os marcadores de adesão, por sua vez, detectam precocemente vulnerabilidades na adesão ao tratamento de doentes com TB, por meio de escores, apresentando forte potencialidade para o monitoramento dessa adesão no âmbito da Atenção Primária à Saúde (APS), aprimorando a vigilância de pessoas com TB. OBJETIVO: Ver...

  14. AS NECESSIDADES DOS ALUNOS AO CUIDAR EM ENFERMAGEM: UMA ANÁLISE COMPREENSIVA

    OpenAIRE

    Silva Pinto, Ana Cristina; UNIRIO; Da Silva, Teresinha De Jesus E. Santo; UNIRIO

    2010-01-01

    INTRODUÇÃO: O estudo apresenta dados da Dissertação de Mestrado intitulada: O ALUNO DE ENFERMAGEM NA PRÁTICA ASSISTENCIAL DO CUIDADO – UMA ANÁLISE COMPREENSIVA DE SUAS NECESSIDADES, na qual investigamos o típico da ação dos alunos ao solicitarem a presença do professor quando realizam os cuidados de enfermagem. A fenomenologia compreensiva de Alfred Schutz, permitiu compreender a ação intencional dos alunos ao solicitarem o professor para realizarem sua prática assistencial do cuidado de enfe...

  15. The Ashkenazi Jews of Curaçao, a trading minority

    Directory of Open Access Journals (Sweden)

    Eva Abraham-Van der Mark

    2000-07-01

    Full Text Available First describes the early Sephardi presence in Curaçao, the arrival of the Ashkenazi in the 20th c., and the relations between these 2 groups. Author goes on to discuss the Ashkenazis' economic success and the exodus of the 1980s. She asks whether the success and the exodus can be attributed to the characteristics of the group itself or whether conditions and developments in Curaçao account for economic fortune and the departure of the Ashkenazi.

  16. Rise of the Machines: Automated Laser Guide Star Adaptive Optics Observations of Thousands of Objects with Robo-AO

    Science.gov (United States)

    Riddle, Reed L.; Baranec, C.; Law, N. M.; Tendulkar, S. P.; Ramaprakash, A. N.; Kulkarni, S. R.; Dekany, R.; Bui, K.; Burse, M.; Das, H.; Punnadi, S.; Chordia, P.

    2013-01-01

    Robo-AO is the first fully automated laser guide star adaptive optics instrument. Robo-AO has completed thousands of automated AO observations at the visible diffraction limit for several scientific programs during its first semester of science observations. These programs include: the Ultimate Binarity Survey to examine stellar binarity properties across the main sequence and beyond; a survey of 1,000 Kepler objects of interest; the multiplicity of solar type stars; and several programs for high precision astrometric observations. A new infrared camera is under development for Robo-AO, and a clone of the system is in the planning stages. This presentation will discuss the Robo-AO instrument capabilities, summarize the science programs undertaken, and discuss the future of Robo-AO.

  17. Sociedades de informaçao: indústria e serviços de conteudo: propostas do IBICT

    Directory of Open Access Journals (Sweden)

    José Rincón Ferreira

    2013-11-01

    Full Text Available  Coloca-se o conceito, escopo, abragência e objetivos da Sociedade da Informaçao, propondo-se um referencial para o seu entendimento. Analisam-se algumas propostas em nível mundial e da América Latina. Apresenta-se com a proposta do Instituto Brasileiro de Informaçao em Ciência e Tecnologia, IBICT, que propoe o desenvolvimento de um conjunto de açoes para a área da informaçao nos aspectos de indústria da informaçao e serviços de conteúdo, que permitam a discussao para a construçao da Sociedade da Informaçao no país, contribuindo para o desenvolvimento de áreas estratégicas para o seu crescimento e alavancagem de setores específicos.

  18. The Robo-AO software: Fully autonomous operation of a laser guide star adaptive optics and science system

    CERN Document Server

    Riddle, Reed L; Law, Nicholas M; Tendulkar, Shriharsh P; Baranec, Christoph; Rudy, Alexander R; Sitt, Marland; Arya, Ankit; Papadopoulos, Athanasios; Ramaprakash, A N; Dekany, Richard G; 10.1117/12.925475

    2013-01-01

    Robo-AO is the first astronomical laser guide star adaptive optics (AO) system designed to operate completely independent of human supervision. A single computer commands the AO system, the laser guide star, visible and near-infrared science cameras (which double as tip-tip sensors), the telescope, and other instrument functions. Autonomous startup and shutdown sequences as well as concatenated visible observations were demonstrated in late 2011. The fully robotic software is currently operating during a month long demonstration of Robo-AO at the Palomar Observatory 60-inch telescope.

  19. The Robo-AO software: fully autonomous operation of a laser guide star adaptive optics and science system

    Science.gov (United States)

    Riddle, Reed L.; Burse, Mahesh P.; Law, Nicholas M.; Tendulkar, Shriharsh P.; Baranec, Christoph; Rudy, Alexander R.; Sitt, Marland; Arya, Ankit; Papadopoulos, Athanasios; Ramaprakash, A. N.; Dekany, Richard G.

    2012-07-01

    Robo-AO is the first astronomical laser guide star adaptive optics (AO) system designed to operate completely independent of human supervision. A single computer commands the AO system, the laser guide star, visible and near-infrared science cameras (which double as tip-tip sensors), the telescope, and other instrument functions. Autonomous startup and shutdown sequences as well as concatenated visible observations were demonstrated in late 2011. The fully robotic software is currently operating during a month long demonstration of Robo- AO at the Palomar Observatory 60-inch telescope.

  20. The Significant Relationship between the Arctic Oscillation (AO) in December and the January Climate over South China

    Institute of Scientific and Technical Information of China (English)

    YANG Hui

    2011-01-01

    Using NCEP/NCAR reanalysis data, the China rainfall and surface temperature data of the China Meteorological Administration, and the Arctic Oscillation (AO) indices of NOAA, the author investigates relationships between the AO and the precipitation and temperature over China. There exists a good relationship between the AO index in December and the succeeding January precipitation over South China,indicating that when the December AO index is positive (negative), the January precipitation over South China increases (decreases). A remarkable negative correlation between the December AO index and the January surface temperature also exists over South China, indicating that when the December AO index is positive (negative), the January temperature over South China drops (rises). The occurrence of this anomalous climate is related to the anomalies of the atmospheric circulation systems. The December AO greatly influences circulation anomalies in January. A positive phase of the AO is found to lead to a stronger subtropical jet over the south side from the Iran Plateau to the Tibetan Plateau. Consequently, it results in a deepening pressure trough around the Bay of Bengal, which transports the warm and wet air to South China continuously. The Siberian High in January is stronger and extends farther southeastward. It results in continual cold air at 1000 hPa pouring into South China, inducing low temperature. Cooperating with the trough of the Bay of Bengal, anomalous precipitation occurs over South China. For the negative phase of the December AO, the opposite situation is observed.

  1. Doença periodontal materna como fator associado ao baixo peso ao nascer Maternal periodontal disease as a factor associated with low birth weight

    Directory of Open Access Journals (Sweden)

    Simone Seixas da Cruz

    2005-10-01

    Full Text Available OBJETIVO: Estudos recentes têm apresentado evidências de que a doença periodontal em gestantes pode ser um dos determinantes do baixo peso ao nascer. Realizou-se estudo para verificar a existência de associação entre doença periodontal materna e baixo peso ao nascer. MÉTODOS: Estudo do tipo caso-controle com 302 mulheres, sendo 102 mães de nascidos vivos de baixo peso (grupo caso e 200 mães de nascidos vivos com peso normal (grupo controle. A existência de associação entre doença periodontal e baixo peso ao nascer foi avaliada mediante modelo multivariado de regressão logística, considerando outros fatores de risco para o baixo peso. RESULTADOS: Ambos os grupos de mães eram comparáveis no que se refere a idade, altura, peso pré-gestacional, tabagismo, alcoolismo, doenças prévias, estado civil, situação socioeconômica, número de escovações e uso de fio dental, número de refeições diárias, e visitas ao dentista. A doença periodontal foi diagnosticada em 57,8% das mães do grupo caso e 39,0% do grupo controle. A análise de regressão logística indicou associação positiva entre doença periodontal e baixo peso ao nascer (ORbruto=2,15; IC 95%: 1,32-3,48, especialmente entre as mães com escolaridade menor ou igual a quatro anos (ORajustada=3,98; IC 95%: 1,58-10,10. CONCLUSÕES: A doença periodontal é um possível fator de risco para o baixo peso ao nascer.OBJECTIVE: Recent studies have presented evidence that periodontal disease in pregnant women may be a determining factor for low birth weight. The present investigation was carried out to verify whether or not there is an association between maternal periodontal disease and low birth weight. METHODS: This was a case-control study on 302 women, of whom 102 were the mothers of live newborns of low weight (case group and 200 were the mothers of live newborns of normal birth weight (control group. The existence of an association between periodontal disease and low

  2. Do afro-americanismo ao caucasianismo: um convertido discute o cristianismo ocidental

    Directory of Open Access Journals (Sweden)

    Edison Minami

    2015-04-01

    Foi a partir dessas ideias de fundo que iniciei a leitura do livro de Alex e Donna Jones, Não tem preço. O livro – na verdade são dois textos, um do marido e outro da esposa – relatos da conversão de dois afro-americanos pentecostais ao catolicismo.

  3. O suicídio como questão: melancolia e passagem ao ato

    Directory of Open Access Journals (Sweden)

    Marcos Vinicius Brunhari

    2014-06-01

    Full Text Available O artigo toma a questão do suicídio na obra de Freud como um problema de pesquisa que interroga certos pontos de sua metapsicologia: a possibilidade de uma tendência à autodestruição, o papel do eu e, finalmente, a dimensão do objeto que estaria implicada. É em relação a este último, em particular a seu estatuto na articulação que Freud propõe entre suicídio e melancolia, que o trabalho de Lacan em torno do objeto a, associado à passagem ao ato e à melancolia no Seminário, livro 10 - A angústia (1962-1963/2005, figura, no artigo, como um acréscimo à investigação iniciada por Freud. Essa referência lacaniana permitirá destacar a presença fulgurante do objeto a no momento da passagem ao ato suicida, estabelecendo uma articulação com a identificação melancólica descrita por Freud em seu "Luto e melancolia" (1917 [1915]/1969. A essa identificação absoluta ao objeto em questão é correlata a ausência de um sujeito e a ruptura mais radical em relação ao Outro.

  4. Indicadores comportamentais de propensão ao homicídio em agressores sexuais

    Directory of Open Access Journals (Sweden)

    Christian da Silva Costa

    2012-01-01

    Full Text Available OBJETIVO: Avaliamos se existe associação entre características de personalidade e tipo de estupro cometido (com ou sem a morte da vítima. MÉTODOS: Como estratégias de estudo, utilizaram-se indicadores comportamentais que podem ser associados à personalidade antissocial, de modo a diferenciar os comportamentos externalizantes de agressores sexuais que matam ou não suas vítimas. RESULTADOS: Os agressores sexuais apresentaram comportamentos externalizantes relacionados à personalidade antissocial. O grupo de indivíduos que cometeu estupro seguido de morte tem esses traços comportamentais mais presentes no decorrer da vida. CONCLUSÃO: Concluímos que os agressores sexuais aparentam ter indicadores que permitem uma investigação mais profunda em relação à personalidade antissocial, mas aqueles que matam suas vítimas diferem quanto ao seu histórico comportamental daqueles que não cometem o homicídio subsequente ao ato de estupro. São indicadores comportamentais da propensão ao homicídio em agressores sexuais: estarem, mais comumente, sob os efeitos de álcool/drogas durante o ato de estupro, propensão ao suicídio, prematuridade de início da vida criminosa e alta impulsividade.

  5. High prevalence of dermal parasites among coral reef fishes of Curaçao

    NARCIS (Netherlands)

    Bernal, M.A.; Floeter, S.R.; Gaither, M.R.; Longo, G.O.; Morais, R.; Ferreira, C.E.L.; Vermeij, M.J.A.; Rocha, L.A.

    2016-01-01

    During expeditions to Curaçao in August and October of 2013, a large number of fish infected with dermal parasites was observed. Infected individuals presented black spots and white blemishes on their skin and fins that were easily observed by divers, and which have been associated with infections b

  6. Pseudoscorpions of the genus Aphelolpium (Pseudoscorpionida, Olpiidae) from Curaçao, Aruba and Bonaire

    NARCIS (Netherlands)

    Tooren, van den D.

    1995-01-01

    Tooren, D. van den, 1995. Pseudoscorpions of the genus Aphelolpium (Pseudoscorpionida, Olpiidae) from Curaçao, Aruba and Bonaire. Studies Nat. Hist. Caribbean Region 72. Amsterdam, 1995: 69-97. A large number of new localities of the pseudoscorpion Aphelolpium scitulum Hoff, 1964 from Curacao, Aruba

  7. Exposição ao vivo no tratamento de agorafobia: relato de caso

    Directory of Open Access Journals (Sweden)

    Gustavo J. Fonseca D'El Rey

    Full Text Available Relato de caso, em que a Exposição ao Vivo foi utilizada no tratamento de paciente com diagnóstico de agorafobia. O tratamento foi realizado em doze sessões, apresentando êxito no uso dessa técnica comportamental.

  8. Modelo An\\'alogo Ac\\'ustico ao Buraco Negro de Schwarzschild

    CERN Document Server

    Toniato, Júnior Diniz

    2011-01-01

    Um fluido em movimento pode agir sobre o som da mesma forma que os espa\\c{c}o-tempos curvos podem influenciar na trajet\\'oria da luz na relatividade geral. Com isso, pode-se descrever a propaga\\c{c}\\~ao dessas ondas sonoras atrav\\'es de uma m\\'etrica efetiva, sob a qual elas seguir\\~ao geod\\'esicas nulas. Esta disserta\\c{c}\\~ao faz uma revis\\~ao destes estudos concentrando-se em uma analogia ac\\'ustica para um buraco negro de Schwarzschild, demonstrando suas vantagens e limita\\c{c}\\~oes quando aplicada para o estudo da teoria de Hawking. A fluid in moviment can act on the sound the same way that curved space-time can influence on light trajectory in the general relativity. So, one can describe the propagation these sound waves through an effective metric, under wich they will follow null geodesics. This thesis makes a review of these studies focusing in an analogy to Schwarzschild black hole using an acoustic system, showing its advantages and limitations when applied in the study of Hawking's theory.

  9. Social behavior of the Paguridae and Diogenidae of Curaçao

    NARCIS (Netherlands)

    Hazlett, Brian A.

    1966-01-01

    1. The social behavior patterns of twelve species of hermit crabs found in the waters around Curaçao, N.A. are described. All species showed marked similarity in their aggressive displays, the most common of which are movements of the appendages, called here the ambulatory raise and cheliped extensi

  10. Molecular Evidence for Dissemination of Unique Campylobacter jejuni Clones in Curaçao, Netherlands Antilles

    NARCIS (Netherlands)

    Duim, B.; Godschalk, P.C.R.; Braak, N. van den; Dingle, K.E.; Dijkstra, J.R.; Leyde, E.; Plas, J. van der; Colles, F.M.; Endtz, H.P.; Wagenaar, J.A.; Maiden, M.C.J.; Belkum, A. van

    2003-01-01

    Campylobacter jejuni isolates (n = 234) associated with gastroenteritis and the Guillain-Barré syndrome (GBS) in the island of Curaçao, Netherlands Antilles, and collected from March 1999 to March 2000 were investigated by a range of molecular typing techniques. Data obtained by pulsed-field gel ele

  11. Biology of the Graysby, Epinephelus cruentatus, of the coral reef of Curaçao

    NARCIS (Netherlands)

    Nagelkerken, W.P.

    1979-01-01

    Epinephelus (Cephalopholis) cruentatus (Lacépède, 1802) [Petrometopon cruentatum], the Graysby, is one of the most common groupers on the coral reef at the southwest coast of Curaçao. They are very abundant at a depth from 7½ to 9 m. A second, lower maximum in their vertical distribution is found at

  12. Atomic Oxygen (AO) and Nitrogen (AN) In-situ Flux Sensor

    Science.gov (United States)

    2016-03-10

    AFRL-AFOSR-VA-TR-2016-0126 DURIP 09) AN ATOMIC OXYGEN FLUX MONITOR FOR USE IN THE SEARCH FOR NEW AND BETT Malcolm Beasley LELAND STANFORD JUNIOR UNIV ...Grant # FA9550-01-1-0433 M. R. Beasley, PI Stanford University Project Title: Atomic Oxygen (AO) and Nitrogen (AN) In-situ Flux Sensor

  13. Dynamic SPECT of the brain using a lipophilic technetium-99m complex, PnAO

    DEFF Research Database (Denmark)

    Holm, S; Andersen, A R; Vorstrup, S;

    1985-01-01

    The lipophilic 99mTc-labeled oxime propylene amine oxime (PnAO) should, according to recent reports behave like 133Xe in the human brain. This study compares SPECT images of the two tracers in six subjects: four stroke cases, one transitory ischemic attack case and one normal subject. Technetium-99...

  14. High prevalence of dermal parasites among coral reef fishes of Curaçao

    NARCIS (Netherlands)

    M.A. Bernal; S.R. Floeter; M.R. Gaither; G.O. Longo; R. Morais; C.E.L. Ferreira; M.J.A. Vermeij; L.A. Rocha

    2015-01-01

    During expeditions to Curaçao in August and October of 2013, a large number of fish infected with dermal parasites was observed. Infected individuals presented black spots and white blemishes on their skin and fins that were easily observed by divers, and which have been associated with infections b

  15. A new species of the Rodent Baiomys from Aruba and Curaçao

    NARCIS (Netherlands)

    Husson, A.M.

    1960-01-01

    In his paper on the mammals of the islands of Aruba, Curaçao, and Bonaire (situated off the north coast of Venezuela), WAGENAAR HUMMELINCK (1940 a, p. 69) mentioned a juvenile specimen of a cricetine rodent which was identified by Mr. M. A. C. HINTON and Mr. R. W. HAYMAN as probably belonging to the

  16. Poluição ambiental, residência materna e baixo peso ao nascer

    Directory of Open Access Journals (Sweden)

    Marli Terezinha Stein Backes

    2011-08-01

    Full Text Available Estudo caso-controle, que objetivou analisar os fatores de risco associados ao baixo peso ao nascer de recém-nascidos de mães de Rio Grande-RS residentes nas proximidades da área industrial. Foram entrevistadas mães que deram à luz nas maternidades do município, durante os meses de abril a novembro de 2003. A amostra compreendeu 138 casos e 409 controles. Foi realizada análise estatística bivariada e multivariada. O Baixo Peso ao Nascer (BPN manteve-se associado positivamente com natimortos prévios, BPN prévios, presença de hipertensão arterial durante a gestação e ameaça de aborto durante a gravidez atual. Foi possível identificar os principais fatores de risco a que estão expostas as gestantes e que interferem no peso ao nascer de seus filhos, os quais vêm somar-se àqueles decorrentes de uma maior exposição a poluentes, por residirem próximo às indústrias.

  17. Tenebrionid Beetles of Curaçao, Aruba, Bonaire, and Venezuela

    NARCIS (Netherlands)

    Marcuzzi, Giorgio

    1959-01-01

    This contribution may be considered as an Appendix to my paper on “Tenebrionid Beetles of Curaçao, Aruba, Bonaire, and the Venezuelan Islands”, which was published in the fifth volume of this series (1954). The addition has proved to be justified after study of: 1. a collection of Tenebrionids gathe

  18. Resistência ao clopidogrel: prevalência e variáveis associadas

    Directory of Open Access Journals (Sweden)

    Fabrício Braga da Silva

    Full Text Available FUNDAMENTO: A dupla terapia antiagregante plaquetária com ácido acetilsalicílico e clopidogrel é pedra angular do tratamento de pacientes submetidos a angioplastia com implante de stents coronarianos. Todavia, parte desses pacientes, a despeito do uso de aspirina e clopidogrel, não se encontram eficazmente antiagregados, fenômeno conhecido como resistência aos antiagregantes plaquetários. A sua prevalência, assim como as condições a ela relacionadas são desconhecidas em nosso meio. OBJETIVO: Determinar a prevalência de resistência ao clopidogrel, assim como as variáveis a ela relacionadas. MÉTODOS: Pacientes admitidos para angioplastia eletiva em uso crônico de aspirina e clopidogrel entre janeiro de 2007 e janeiro de 2010. Uma hora após o procedimento, foi medida a agregação plaquetária utilizando a agregometria óptica com difosfato de adenosina 5 µmoles/l como agonista. Nesse momento, em um coorte transversal, determinou-se a prevalência de resistência ao clopidogrel, definida com um valor de agregação plaquetária > 43% e um modelo de regressão logística às variáveis a ela relacionadas. RESULTADOS: Foram analisados 205 pacientes (66,4 ± 11anos, 61,5% masculino. A prevalência de resistência ao clopidogrel foi 38,5% (IC95% 31,9 - 45,2%. O valor da glicemia (OR = 1,014 IC95% 1,004 - 1,023, infarto do miocárdio prévio (OR = 2,320 IC95% 1,1103 - 4,892 e a resposta terapêutica à aspirina (OR = 1,057 IC95% 1,017 - 1,099 foram as variavéis de associação independente à resistência ao clopidogrel. CONCLUSÃO: A prevalência de resistência ao clopidogrel foi alta. Glicemia, infarto agudo do miocárdio prévio e a resposta ao ácido acetilsalicílico foram variáveis a ela relacionadas. A melhor compreensão desse fenômeno se faz necessária frente às novas propostas de antiagregantes plaquetários.

  19. Perfilhamento em milho: processo benéfico ou prejudicial ao desenvolvimento da planta e ao rendimento de grãos? Lages – SC 2008

    OpenAIRE

    Amauri Schmitt

    2008-01-01

    Os perfilhos são estruturas importantes para muitas poáceas, pois aumentam o número de inflorescências por área e suprem fotoassimilados ao colmo principal. Na cultura do milho, os perfilhos têm sido historicamente considerados estruturas indesejáveis, pois normalmente não produzem espigas. O lançamento de híbridos que apresentam alto potencial produtivo e capacidade de emissão de perfilhos, suscitou dúvidas sobre a real influência dos perfilhos sobre a performance agronômica do milho. Esse t...

  20. Baixo peso ao nascer em duas coortes de base populacional no Sul do Brasil

    Directory of Open Access Journals (Sweden)

    Bernardo L. Horta

    Full Text Available As crianças com baixo peso ao nascer (menos de 2.500 g apresentam um risco muitas vezes maior de morrer ou adoecer no primeiro ano de vida. O presente estudo teve como objetivo comparar a ocorrência de baixo peso ao nascer, nascimento pré-termo e retardo de crescimento intra-uterino, nos anos de 1982 e 1993, em Pelotas, Rio Grande do Sul. Nestes dois anos foram avaliados todos os nascimentos hospitalares, que representam mais de 99% do total dos nascimentos. O baixo peso ao nascer aumentou de 9,0% para 9,8% em 1993 (p=0,2, os nascimentos pré-termo aumentaram de 5,6% em 1982 para 7,5% em 1993 (p<0,01 e o retardo de crescimento intra-uterino passou de 15,0% em 1982 para 17,5% em 1993 (p<0,05. Nos dois períodos estudados, a renda familiar esteve inversamente associada com o risco de baixo peso e retardo de crescimento intra-uterino, mas não com nascimentos pré-termo. Em 1993, apesar da melhoria nas condições sócio-econômicas e nutricionais das mães, como também do aumento no número de consultas pré-natais, observou-se um crescimento na prevalência de baixo peso ao nascer que não foi significativa na análise bivariada. Entretanto, após ajuste para possíveis variáveis de confusão, por meio de regressão logística, houve um aumento no risco de baixo peso ao nascer da ordem de 33% (p<0,01.

  1. Resistência de Eleusine indica aos inibidores de ACCase Eleusine indica resistance to ACCase inhibitors

    Directory of Open Access Journals (Sweden)

    R.A. Vidal

    2006-01-01

    Full Text Available Dentre as causas da ineficácia no controle de plantas daninhas destaca-se a resistência delas aos herbicidas. Os objetivos deste trabalho foram avaliar a suspeita de resistência de Eleusine indica a inibidores de acetil-CoA carboxilase (ACCase e investigar a ocorrência de resistência cruzada entre os inibidores de ACCase. Biótipo de Eleusine indica originado do Mato Grosso com suspeita de resistência aos herbicidas inibidores de ACCase foi avaliado em casa de vegetação na sua suscetibilidade para diversos produtos do grupo dos ariloxifenoxipropionatos e cicloexanodionas. Estudos de resposta à dose confirmaram que o biótipo era 18 vezes mais insensível ao sethoxydim do que biótipo suscetível nunca aspergido com herbicidas. Também se constatou resistência cruzada ao fenoxaprop, cyhalofop, propaquizafop e butroxydim. Não se observou resistência cruzada aos produtos fluazifop, haloxyfop, quizalofop e clethodim.Among the causes for weed control inefficacy, the worst one is resistance to herbicides. The objectives of this work were to evaluate an Eleusine indica biotype suspected of resistance to ACCase inhibitors and to investigate the occurrence of cross- resistance to several ACCase inhibitors. One biotype of Eleusine indica originated from Mato Grosso with suspected resistance to ACCase inhibitors was evaluated in a greenhouse in relation to its susceptibility to several products of the ariloxyphenoxypropionate and cyclohexanedione groups. Studies on dose response confirmed that the suspected biotype was 18 times more insensitive to sethoxydim than the susceptible biotype that had never been treated with herbicides. Cross-resistance was confirmed for fenoxaprop, cyhalofop, propaquizafop and butroxydim. No cross-resistance was observed with fluazifop, haloxyfop, quizalofop, and clethodim.

  2. Aumento da imunorreatividade ao VEGFR-1 no complexo coroido-escleral em modelo experimental de hipercolesterolemia

    Directory of Open Access Journals (Sweden)

    Rogil José de Almeida Torres

    2013-02-01

    Full Text Available OBJETIVO: O objetivo deste trabalho é investigar a expressão do fator de crescimento vascular endotelial (VEGF na coroide e esclera, utilizando um modelo experimental de hipercolesterolemia. MÉTODO: Coelhos New Zealand foram organizados em dois grupos: O grupo dieta normal (GN, composto por 8 coelhos (8 olhos, recebeu ração padrão para coelhos, durante 4 semanas; e o grupo hipercolesterolêmico (GH, composto por 13 coelhos (13 olhos, recebeu dieta rica em colesterol a 1% por 8 semanas. Foi realizada a dosagem sérica de colesterol total, triglicerídeos, HDL colesterol, glicemia de jejum no início do experimento e no momento da eutanásia. Ao final da 8ª semana para o GH e 4ª semana para o GN foi realizada a eutanásia dos animais e os olhos foram submetidos à análise imuno-histoquímica com os anticorpos RAM-11 e VEGFR-1. RESULTADOS: Observou-se significativo aumento do colesterol total e triglicerídeos do GH em relação ao GN (p<0,001. Houve significativo aumento da expressão da RAM-11 e VEGFR-1 na coroide e esclera dos animais do GH em relação ao GN (p<0,001. CONCLUSÃO: Este estudo demonstra que a dieta hipercolesterolêmica em coelhos induz ao aumento da concentração de macrófagos e da imunorreatividade ao VEGFR-1 na coroide e esclera, expressando similaridade com a degeneração macular relacionada à idade (DMRI humana.

  3. A new species of Hemicyclops (Crustacea, Copepoda, Poecilostomatoida, Clausidiidae) associated with hermit crabs in Curaçao

    NARCIS (Netherlands)

    Stock, Jan H.

    1992-01-01

    STOCK, J. H. 1992. A new species of Hemicyclops (Crustacea, Copepoda, Poecilostomatoida, Clausidiidae) associated with hermit crabs in Curaçao. Stud. Nat. Hist. Caribbean Region 71, Amsterdam 1992: 69-78. Hemicyclops geminatus n. sp. is described from the upper infralittoral zone of Curaçao (Antille

  4. Resistência de genótipos de caupi ao caruncho

    Directory of Open Access Journals (Sweden)

    BARRETO PAULO DIÓGENES

    2000-01-01

    Full Text Available A utilização de resistência genética ao ataque de Callosobruchus maculatus (Fabr. tem sido alvo de investigação científica, especialmente no que diz respeito à identificação de fontes de resistência. O presente trabalho objetivou incorporar, ao grupo de caracteres desejáveis para o cultivo de caupi (Vigna unguiculata (L. Walp., resistência genética ao caruncho (C. maculatus. Foram realizadas hibridações dos genótipos IT81D-1045 e IT81D-1064 (portadores de resistência ao inseto com CNCx 252-1E/FB, CNCx 187-22D-1 e BR 1-Poty (capazes de transferir resistência a viroses, tolerância à seca, formação de grãos com padrão comercial, elevado potencial de produção e adaptabilidade a diferentes condições ambientais. Populações segregantes obtidas destes cruzamentos foram conduzidas pelo método SPD (descendência de uma única vagem, e na geração F5 foram realizadas seleções individuais. As linhagens obtidas foram avaliadas em conjunto com materiais de origens diferentes, utilizando-se parâmetros associados à infestação da praga. Foi constatado que os genótipos avaliados apresentaram variabilidade quanto à preferência à postura, número de insetos emergidos e número de sementes danificadas; as linhas EVx 37-15E e EVx 37-2E foram as que sofreram menor dano causado pelo caruncho; as variáveis número de ovos, número de insetos emergidos e número de sementes danificadas mostraram-se positiva e significativamente correlacionadas entre si; o grupo das linhagens que descendem de genitores resistentes apresenta valores significativamente inferiores aos obtidos pelas demais, o que indica que a resistência ao inseto se transmite geneticamente.

  5. Medo, ansiedade e controle relacionados ao tratamento odontológico

    Directory of Open Access Journals (Sweden)

    SINGH Kira Anayansi

    2000-01-01

    Full Text Available Nosso objetivo foi avaliar medo, ansiedade e controle relacionados ao tratamento odontológico. Os sujeitos foram 364 crianças da faixa etária de 7 a 13 anos. Três questionários com questões de múltipla escolha foram aplicados em grupos de 10 crianças. O primeiro questionário destinou-se à avaliação do medo ao tratamento odontológico e outras situações. Foi traduzido e adaptado do "Child's Fear Survey Schedule"9 e contém 15 itens. O segundo questionário contém 20 itens relacionados as situações potencialmente produtoras de ansiedade. Foi traduzido e adaptado do "State Trait Anxiety Inventory for Children"16. O terceiro questionário contém 40 itens sendo 20 relacionados ao controle percebido e 20 ao controle desejado e foi traduzido e adaptado do "Child Dental Control Assessment"19. Em relação ao medo e ansiedade, a média dos escores foi mais elevada para o sexo feminino do que para o sexo masculino (P < 0,05. Em relação a idade, as crianças da faixa de 11 a 13 anos revelaram-se em média mais temerosas que as de 7 a 9 anos. Quanto ao controle percebido as crianças mais novas percebem mais controle do que as mais velhas. Em geral os dados indicam que valores mais altos de medo relacionam-se com valores mais baixos de controle percebido. As crianças que tinham realizado tratamento odontológico com anestesia mostraram-se mais temerosas do que aquelas que não foram submetidas a anestesia, o que permite inferir que a etiologia de medo pode relacionar-se a esse procedimento que pode envolver a percepção de incontrolabilidade.

  6. Isolation and characterization of an agaro-oligosaccharide (AO-hydrolyzing bacterium from the gut microflora of Chinese individuals.

    Directory of Open Access Journals (Sweden)

    Miaomiao Li

    Full Text Available Agarose (AP from red algae has a long history as food ingredients in East Asia. Agaro-oligosaccharides (AO derived from AP have shown potential prebiotic effects. However, the human gut microbes responsible for the degradation of AO and AP have not yet been fully investigated. Here, we reported that AO and AP can be degraded and utilized at various rates by fecal microbiota obtained from different individuals. Bacteroides uniformis L8 isolated from human feces showed a pronounced ability to degrade AO and generate D-galactose as its final end product. PCR-DGGE analysis showed B. uniformis to be common in the fecal samples, but only B. uniformis L8 had the ability to degrade AO. A synergistic strain, here classified as Escherichia coli B2, was also identified because it could utilize the D-galactose as the growth substrate. The cross-feeding interaction between B. uniformis L8 and E. coli B2 led to exhaustion of the AO supply. Bifidobacterium infantis and Bifidobacterium adolescentis can utilize one of the intermediates of AO hydrolysis, agarotriose. Growth curves indicated that AO was the substrate that most favorably sustained the growth of B. uniformis L8. In contrast, κ-carrageenan oligosaccharides (KCO, guluronic acid oligosaccharides (GO, and mannuronic acid oligosaccharides (MO were found to be unusable to B. uniformis L8. Current results indicate that B. uniformis L8 is a special degrader of AO in the gut microbiota. Because B. uniformis can mitigate high-fat-diet-induced metabolic disorders, further study is required to determine the potential applications of AO.

  7. Truncus arteriosus operado aos 28 anos: importância do diagnóstico diferencial

    Directory of Open Access Journals (Sweden)

    Lilian Maria Lopes

    2011-08-01

    Full Text Available Descrevemos um caso de adulto de 28 anos com suspeita de cardiopatia congênita desde o nascimento, não tratada na infância por opção da família. Aos 27 anos, foi feito diagnóstico de atresia pulmonar com comunicação interventricular e colaterais sistêmico-pulmonares, sendo contraindicada a cirurgia. Uma nova reavaliação em nosso serviço demonstrou tratar-se de um truncus arteriosus atípico. O fato de um tronco arterial comum com shunt esquerda-direita ter sido visualizado ao ecocardiograma foi um dado crucial para a indicação de novo cateterismo, abrindo perspectiva de correção cirúrgica. No momento, o paciente encontra-se bem, com 7 anos de evolução pós-operatória.

  8. O exercício do estudar nos cursinhos destinados aos concursos públicos

    Directory of Open Access Journals (Sweden)

    Leonardo Del Puppo Luz

    2008-06-01

    Full Text Available O artigo discute o processo de produção da subjetividade contemporânea elaborado sobre o Estudar em alunos de cursinhos destinados aos concursos públicos. Procura-se discutir as tecnologias de poder, sobretudo na relação saber-poder, engendradas nestes "ambientes educacionais", já que tais construções sobre o Estudar produzem e perpetuam a manifestação de conceitos absolutos e verdadeiros de se pensar o estudo por meio de técnicas e procedimentos de funcionamentos ideais e idealizados. Busca-se problematizar este modelo homogêneo baseado no tecnicismo do estudar e nas falas sobre o verdadeiro visando colocar em análise o estereótipo do "fracasso" atrelado aos alunos vinculados a estes locais.

  9. Alta cozinha e arte: da nutrição ao degustar artístico

    OpenAIRE

    2015-01-01

    Mestrado em Gestão e Estudos da Cultura Ao longo do presente trabalho analisa-se parte da complexa relação existente entre a gastronomia e a arte. A análise inicia-se com uma breve introdução à histórica da gastronomia, desde o período pré-histórico até à alta cozinha contemporânea, identificando diferentes momentos que, direta ou indiretamente, promoveram a aproximação da gastronomia ao universo artístico. A análise prossegue pela estética da arte, aferindo, de forma breve,...

  10. As intervenções do terapeuta ao longo de uma psicoterapia

    OpenAIRE

    Dias, Sandra C. Ribeiro Amaral

    2009-01-01

    Dissertação de Mestrado apresentada ao ISPA - Instituto Universitário Esta revisão de literatura teve por objectivo a pesquisa, a organização e a conceptualização de informação relativamente à técnica psicoterapêutica, especificamente numa abordagem psicanalítica. No entanto, o estudo da técnica foi enquadrado na investigação da psicoterapia ao nível dos processos e produtos. Tentou-se perceber e sistematizar os conceitos base da técnica clássica psicanalítica e a sua contraposição em rela...

  11. DM dimensioning for the next generation AO systems : strategies and rules

    Science.gov (United States)

    Costille, A.; Conan, J.-M.

    2007-07-01

    Adaptive optics (AO) provides a real time correction of turbulence and allows to improve the angular resolution of the astronomical telescopes. The design of AO systems and of its key components (deformable mirror (DM), wave-front sensors...) is very important to provide the best performance. The definition rules are well known for 10m class telescopes. However the study of the next generation of telescopes, especially the Extremely Large Telescope (ELT), associated with the development of new technological solutions, particularly concerning DMs, require to refine and in some cases to reconsider these rules. Both turbulent parameters (seeing, outer scale ...), telescope effects, system design and performance requirements have to be considered in order to define the DM key parameters (stroke, inter-actuator stroke, influence function ...). We present a study based on the combination of analytical expressions and numerical simulation results. We do not give absolute rules of design but we present key aspects to keep in mind when designing the DM.

  12. The role of AO external fixation in proximal femoral osteotomies in the pediatric neuromuscular population.

    Science.gov (United States)

    Handelsman, John E; Weinberg, Jacob; Razi, Afshin; Mulley, Debra A

    2004-09-01

    Internal fixation in proximal femoral osteotomies using traditional devices may be sub-optimal in children with neuromuscular disorders who have small or osteopenic bone. In this population, between 1988 and 2000, we performed 36 proximal femoral varus osteotomies in 28 patients. These were controlled by the AO external fixator. The average age at surgery was 7 years (range, 2-13 years). A mean varus correction of 34 degrees (range, 15-90 degrees) was obtained. Complications consisted of one superficial pin tract infection, one skin breakdown, and one non-union. Other than the non-union, all osteotomies were stable at the time of the fixator removal. The AO external fixator is an effective alternative in maintaining corrective proximal femoral osteotomies in children with fragile bones.

  13. Evaluation of the reproducibility of the AO/ASIF classification for humeral shaft fractures☆

    Science.gov (United States)

    Pignataro, Gustavo Soriano; Junqueira, André Elias; Matsunaga, Fabio Teruo; Matsumoto, Marcelo Hide; Belloti, João Carlos; Tamaoki, Marcel Jun Sugawara

    2015-01-01

    Objective To evaluate the reproducibility of the AO/Asif classification for humeral shaft fractures. Methods Consecutive radiographs of the arm in both anteroposterior and lateral view from 60 patients with humeral shaft fractures were analyzed. Six observers who were familiar with the AO/Asif classification (three shoulder and elbow surgery specialists and three general orthopedists) were selected to make the analysis, which was done at three different times. The data were subjected to statistical analysis using the kappa coefficient. Results The intra and interobserver concordance was statistically significant in all the analyses. Conclusions All the evaluators showed concordance between the three evaluations that was considered to be statistically significant. However, the highest values were found among the specialists. PMID:26417565

  14. The CfAO's Astronomy Course in COSMOS: Curriculum Design, Rationale, and Application

    CERN Document Server

    Cooksey, Kathy L; Porter, Jason; Raschke, Lynne; Severson, Scott; Hinkley, Sasha

    2010-01-01

    From 2001 to 2007, COSMOS provided a teaching and outreach venue for the Center for Adaptive Optics Professional Development Program (CfAO PDP). COSMOS is a four-week residential mathematics and science summer program for high-school students organized by the University of California on four of its campuses. Two topical science courses comprised each COSMOS cluster. An astronomy course has always formed a basis for the CfAO PDP-affiliated cluster. The course included a variety of pedagogical techniques to address a diversity of learners and goals. We outline the astronomy course---lectures, activities, etc.---and provide the rationale for what was taught, how it was aught, and when it was taught.

  15. Escala multidimensional aplicada aos estudos de apreciação musical

    Directory of Open Access Journals (Sweden)

    Raquel Cocenas da Silva

    2009-08-01

    Full Text Available A escala multidimensional (MDS provém de uma família de técnicas de análise de proximidade de dados, obtida por meio do julgamento do participante que compara vários estímulos em vários traços, concomitantemente. A análise dos julgamentos de proximidade produz um espaço em que pontos representam a relação entre os estímulos em um espaço euclidiano. O ordenamento dos estímulos ao longo das dimensões neste espaço permite que inferências possam ser feitas sobre o universo perceptual subjacente ao grupo de pesquisa. A literatura mostra que a MDS vem ampliando o estudo da música e a mensuração de suas propriedades estruturais.

  16. CANARY phase B: on-sky open-loop tomographic LGS AO results

    Science.gov (United States)

    Morris, Tim; Gendron, Eric; Basden, Alastair; Martin, Olivier; Osborn, James; Henry, David; Hubert, Zoltan; Sivo, Gaetano; Gratadour, Damien; Chemla, Fanny; Sevin, Arnaud; Cohen, Matthieu; Younger, Eddy; Vidal, Fabrice; Wilson, Richard; Butterley, Tim; Bitenc, Urban; Reeves, Andrew; Bharmal, Nazim; Raynaud, Henri-François; Kulcsar, Caroline; Conan, Jean-Marc; Huet, Jean-Michel; Perret, Denis; Dickson, Colin; Atkinson, David; Bailie, Tom; Longmore, Andy; Todd, Stephen; Talbot, Gordon; Morris, Simon; Rousset, Gérard; Myers, Richard

    2014-07-01

    CANARY is an on-sky Laser Guide Star (LGS) tomographic AO demonstrator that has been in operation at the 4.2m William Herschel Telescope (WHT) in La Palma since 2010. In 2013, CANARY was upgraded from its initial configuration that used three off-axis Natural Guide Stars (NGS) through the inclusion of four off-axis Rayleigh LGS and associated wavefront sensing system. Here we present the system and analysis of the on-sky results obtained at the WHT between May and September 2014. Finally we present results from the final `Phase C' CANARY system that aims to recreate the tomographic configuration to emulate the expected tomographic AO configuration of both the AOF at the VLT and E-ELT.

  17. Development and Study on Nitrogen Removal Controller in A/O Process

    Institute of Scientific and Technical Information of China (English)

    MA Yong; PENG Yong-zhen; WANG Shu-ying

    2004-01-01

    In this paper three controllers for A/O process are developed, including a DO cascade controller, an external carbon flow rate controller and an internal recycling flow rate controller. The objective of the different controllers is to control the nitrate and ammonia concentration. Simulation study demonstrated that these controllers could efficiently control nitrogen removal and meet stricter effluent quality standards at a minimum cost.

  18. Prevalencia de nascimentos pre-termo por peso ao nascer: revisao sistematica

    Directory of Open Access Journals (Sweden)

    Mariangela F Silveira

    2013-10-01

    Full Text Available OBJETIVO Estimar a prevalência de nascimentos pré-termo por faixas de peso ao nascer e obter uma equação para correção de estimativas. MÉTODOS Revisão sistemática da literatura nacional, de 1990 a 2012, para identificar estudos com coleta primária de informações sobre peso ao nascer e idade gestacional. Foram selecionados 12 que contribuíram com tabulações da prevalência de nascimentos pré-termo para faixas de 100 g de peso ao nascer. Os resultados desses estudos foram combinados pelo método de polinômios fracionais, sendo obtidas curvas separadas para meninos e meninas, comparadas com os resultados do Sistema de Informações sobre Nascidos Vivos para os anos 2000, 2005, 2010 e 2011. RESULTADOS As estimativas da prevalência de nascimentos pré-termo, obtidas a partir dos estudos primários, foram superiores às do Sistema de Informações sobre Nascidos Vivos para praticamente todas as faixas de peso ao nascer. A prevalência relatada pelo Sistema de Informações sobre Nascidos Vivos foi de 7,1% em 2010, cerca de 38% menor do que a estimativa de 11,7% obtida com a equação de correção. CONCLUSÕES Os dados do Sistema de Informações sobre Nascidos Vivos quanto à prevalência de nascimento pré-termo não refletem a verdadeira dimensão da prematuridade no Brasil. Assim sendo, para sua utilização, será necessária a aplicação do fator de correção, conforme proposto.

  19. Avaliação da sensibilidade da goiabeira 'Pedro Sato' ao ozônio

    Directory of Open Access Journals (Sweden)

    Regina Maria de Moraes

    2011-09-01

    Full Text Available O objetivo deste trabalho foi avaliar a sensibilidade da goiabeira 'Pedro Sato' ao ozônio (O3, em comparação à cultivar Paluma, descrita como sensível. Oito plantas de cada cultivar foram submetidas separadamente a ar filtrado e a ar filtrado + O3, cinco horas por dia, durante quatro dias. Foram avaliadas fotossíntese (Asat, injúrias foliares visíveis e concentração de antocianinas e taninos. Plantas das duas cultivares também foram expostas, durante três meses, a condições ambientais em local contaminado por O3, tendo-se avaliado fotossíntese, injúrias foliares e crescimento. Plantas fumigadas com O3 apresentaram redução de Asat e da atividade fotoquímica, além de manifestação de injúrias foliares. 'Paluma' apresentou maior redução de Asat, injúrias foliares mais severas e redução de taninos. Quando exposta ao ambiente contaminado, 'Paluma' apresentou trocas gasosas mais altas, mas apresentou redução de Asat 30 dias antes que 'Pedro Sato'. O crescimento não foi afetado em 'Pedro Sato', cujas injúrias foliares tiveram menor incidência, severidade e precocidade do que em 'Paluma'. A menor taxa de crescimento de 'Paluma' indica maior sensibilidade ao O3. Apesar de responder com menor intensidade ao estresse induzido pelo O3, a cultivar Pedro Sato não pode ser considerada tolerante, pois apresenta danos fotoquímicos, injúrias foliares e alterações nos conteúdos de metabólitos secundários quando exposta a esse poluente.

  20. Do valor intrínseco e de sua aplicabilidade ao meio ambiente

    Directory of Open Access Journals (Sweden)

    Tânia Ap. Kuhnen

    2004-01-01

    Full Text Available O tema deste estudo é a teoria do valor sagrado da vida e sua aplicabilidade às questões de ética prática, mais especificamente ao meio ambiente. O objetivo geral consiste em apontar os limites da concepção de Dworkin sobre a teoria do sagrado, quando aplicada ao meio ambiente, e a solução que Singer oferece para a atribuição de valor ao mesmo. Justifica-se esse trabalho em decorrência da importância de se discutir, a partir da análise dessas teorias filosóficas, questões vinculadas ao estabelecimento de um princípio ético que, além de englobar, no campo da ação moral, todos os seres sencientes, envolva também o meio ambiente como um todo. Além disso, o tema que aqui se desenvolve é uma contribuição do raciocínio filosófico-teórico para a discussão de problemas relevantes no campo da ética prática. Esse estudo ainda se justifica por abordar a questão da linha moral divisória entre os seres que são ou não incluídos na comunidade moral, o que representa outro problema que concerne à ética. As diferentes formas de classificação não foram capazes de incluir todos os seres vivos no âmbito da comunidade moral. Há sempre exclusões, as quais tendem a diminuir com o surgimento de teorias éticas inovadoras, dentre elas as que serão abordadas a seguir.

  1. World-wide deployment of Robo-AO visible-light robotic laser adaptive optics systems

    Science.gov (United States)

    Baranec, Christoph; Riddle, Reed; Law, Nicholas Michael; Lu, Jessica R.; Tonry, John; Tully, R. Brent; Wright, Shelley; Kulkarni, Shrinivas; Severson, Scott; Choi, Philip; Ramaprakash, A.; Chun, Mark; Connelley, Mike; Tokunaga, Alan; Hall, Donald

    2015-08-01

    In the next few years, several modest-sized telescopes around the world will be upgraded with autonomous laser adaptive optics systems based on the Robo-AO prototype deployed at the Palomar Observatory 1.5-m telescope. The prototype commenced scientific operations in June 2012 and more than 19,000 observations have since been performed at the ~0.12" visible-light diffraction limit. We are planning to move the prototype system to the 2.1-m telescope at Kitt Peak for a 3-year deployment which will serve a consortium of users including Caltech, the University of Hawai`i, IUCAA, NCU and institutions in China. Additionally, 2 months per year will be made available to the US astronomical community.New Robo-AO systems are in various stages of development: a clone by IUCAA for the 2-m IGO telescope in India; a natural guide star variant, KAPAO, by Pomona College at the 1-m Table Mountain telescope in California; and second generation Robo-AO systems are planned for the 3-m IRTF and 2.2-m University of Hawai'i telescopes on Maunakea, Hawai`i. The latter will exploit Maunakea's excellent observing conditions to provide higher Strehl ratios, sharper imaging, ~0.07", and correction to lambda = 400 nm. An additional infrared integral-field spectrograph will be fed by the UH 2.2-m Robo-AO system to quickly classify transients, such as supernovae and asteroids, discovered by the ATLAS system in Hawai`i.

  2. Perícia contábil: uma ferramenta de combate ao crime organizado

    Directory of Open Access Journals (Sweden)

    IDALBERTO JOSÉ DAS NEVES JÚNIOR

    2011-11-01

    Full Text Available No Brasil e no mundo, devido principalmente à ausência do Estado no cumprimento do seu papel de garantidor da lei e da ordem, as organizações criminosas cresceram significativamente, e as suas ações passaram a ocorrer de formas planejadas e estratégicas. Para combatê-las e reprimi-las, a Perícia Contábil, materializada nos laudos periciais elaborados pelos peritos criminalistas, surge como importante ferramenta de Inteligência. Nesse contexto, o objetivo desta pesquisa foi identificar a relevância da Perícia Contábil como meio de prova para a solução de controvérsias relativas ao crime organizado. Para tanto, houve a realização de pesquisa de campo por meio de questionário aplicado aos Delegados, Escrivães e Agentes de Polícia das divisões da Diretoria de Combate ao Crime organizado – DCOR do Departamento de Polícia Federal. Também, houve a utilização de técnicas de estatística multivariada de análise de cluster para o estudo dos registros (K-Means clusters do software SPSS, que resultou na formação de dois agrupamentos. Considerando a pesquisa de campo e o referencial teórico, verificou-se que a Perícia Contábil, no limite de suas atribuições, é um relevante meio de prova para a solução de controvérsias relativas ao crime organizado.

  3. Subordinate but proud: Curaçao's free blacks and mulattoes in the eighteenth century

    Directory of Open Access Journals (Sweden)

    Wim Klooster

    1994-07-01

    Full Text Available Focuses on the socio-economic position of free non-whites in 18th-c. Curaçao. The manumission rate was relatively high and consequently a large group of free non-whites was created. Blacks and mulattoes enjoyed religious freedom as well as considerable economic freedom. Their growing numbers and social and economic assertiveness alarmed the whites, who gradually admitted the most successful mulattoes in their circles.

  4. Do corpo histérico ao "corpus" teórico

    Directory of Open Access Journals (Sweden)

    Francisco Ramos de Farias

    1992-09-01

    Full Text Available Este estudo aborda a maneira pela qual Freud, de forma original, efetuou uma leitura no corpo-sintoma da histérica e construiu uma clínica inédita destinada ao tratamento das neuroses. Esta clínica, fundada na transferência, é suportada pelo edifício teórico que tem o inconsciente e a sexualidade como paradigmas.

  5. Estudo teorico dos aspectos prosturais e da plasticidade muscular frente ao alongamento

    OpenAIRE

    Fabio Chagas Leoni

    1996-01-01

    Resumo: Este estudo teve como objetivo analisar o comportamento e os processos adaptativos musculares frente ao alongamento. Procurou também, observar quais interferências, esta atividade (o alongamento) teria sobre a postura. A atividade de alongamento foi considerada sob a perspectiva de Lê Boulch (1983), como parte integrante de uma educação fundamental pelo movimento, buscando construir a aresta central da personalidade, constituída pelo Esquema Corporal. Trata-se de uma pesquisa bibliogr...

  6. Software interativo: ecocardiografia na avaliaçao da hipertensao arterial

    Directory of Open Access Journals (Sweden)

    Fábio Abdalla Segamarchi

    Full Text Available A hipertensao arterial é uma doença muito prevalente, evoluindo com lesoes em órgaos-alvo, alta morbidade e mortalidade. A avaliaçao das repercussoes cardíacas pela ecocardiografia tem papel importante na conduçao clínica dos indivíduos hipertensos, e suas imagens podem ser utilizadas como ferramenta para o ensino. OBJETIVOS: Desenvolver e avaliar um software, construído com base em imagens ecocardiográficas, abordando de forma ilustrativa e interativa a estrutura e funçao cardíacas normais,bem como as alteraçoes induzidas pela hipertensao arterial. MÉTODOS: Foram selecionadas imagens ecocardiográficas de indivíduos normais e com comprometimento cardíaco determinado pela hipertensao (hipertrofia ventricular esquerda, miocardiopatia dilatada e infarto do miocárdio. Com estas imagens foi construído um software no programa multimídia Flash, que foi avaliado por estudantes de Medicina (n=38 e Enfermagem (n=18, também submetidos a pré- e pós-teste. RESULTADOS: Os alunos consideraram o software útil, atraente e adequado as atuais metodologias educacionais proativas,promovendo ganho de conhecimento na compreensao do comprometimento cardíaco na hipertensao arterial. Conclusao: O software foi bem avaliado e considerado útil na aquisiçao de conhecimento numa área específica mais prevalente do comprometimento da saúde do adulto.

  7. Abscesso subdural ao nível da cauda equina: relato de um caso

    Directory of Open Access Journals (Sweden)

    Antonio Cesar G. Borges

    1977-09-01

    Full Text Available É relatada a observação de um paciente de 42 anos, com paraplegia flácida devida a abscesso subdural ao nível da cauda equina. É realçada a pobreza de alterações sensitivas e esfincterianas neste caso e enfatizada a importância da cirurgia para obtenção de bons resultados.

  8. Análise de fatores associados ao significado do trabalho

    Directory of Open Access Journals (Sweden)

    Sergio Hideo Kubo

    2012-12-01

    Full Text Available O trabalho tem sido visto não somente como forma de obter a renda, mas também como atividade que proporciona realização pessoal, status social e possibilidade de estabelecer e manter contatos interpessoais, entre outros. Nesta pesquisa, teve-se como objetivo investigar os fatores que influenciam e conferem sentido ao trabalho, como centralidade do trabalho, normas da sociedade e objetivos e resultados valorizados. Na centralidade do trabalho, procurou-se investigar o grau de importância do trabalho dentro do contexto das diversas áreas da vida das pessoas, como família, lazer, religião e vida comunitária. Em normas da sociedade, foram analisados os pontos mais significativos no tocante ao que a sociedade deveria proporcionar ao indivíduo, assim como o que o indivíduo deveria fazer em prol da sociedade. Nos objetivos e resultados valorizados, foi pesquisado o que as pessoas buscam com o trabalho. A partir da pesquisa na literatura, foi elaborado um modelo inicial que, não se mostrando satisfatório segundo critérios estatísticos, foi substituído por outro que apresentou significância estatística e boa aderência aos dados. O modelo escolhido foi o que melhor goodness-of-fit apresentou, quando se utilizou modelagem de equações estruturais pelo método partial least square. O estudo revelou que o significado do trabalho se reflete, na ordem, na centralidade do trabalho, nos objetivos e resultados valorizados e, por último, nas normas sociais.

  9. Storage Stability of Jet Fuel Not Containing Anti-Oxidant (AO)

    Science.gov (United States)

    2012-01-31

    hydrotreated jet fuels (with and without AO added at the refinery) and a fuel from Australia thought to have poor thermal stability. These fuels were stored at...at lower temperatures (such as highly hydrotreated fuels) tend to produce smaller amounts of oxidative deposits. Henegan and Zabarnick proposed that...amended to require the addition of oxidation inhibitors to all JP-5 and JP-4 fuels containing hydrotreated blending stocks. The additive inclusion

  10. Assessment of Assembling Objects (AO) for Improving Predictive Performance of the Armed Forces Qualification Test

    Science.gov (United States)

    2011-04-01

    Although the ASVAB has not historically been predictive of attrition ( Laurence , Naughton, & Harris, 1996), the AO subtest has indeed shown...internal consistency (e.g., Earles & Ree , 1992). Alderton and colleagues (1997) found that PC had fairly low test-retest reliability, which was well... Ree (1992) found that WK is a slightly better predictor of course grades than PC across a variety of job types. Mirroring the research on the two

  11. Bridging FPGA and GPU technologies for AO real-time control

    Science.gov (United States)

    Perret, Denis; Lainé, Maxime; Bernard, Julien; Gratadour, Damien; Sevin, Arnaud

    2016-07-01

    Our team has developed a common environment for high performance simulations and real-time control of AO systems based on the use of Graphics Processors Units in the context of the COMPASS project. Such a solution, based on the ability of the real time core in the simulation to provide adequate computing performance, limits the cost of developing AO RTC systems and makes them more scalable. A code developed and validated in the context of the simulation may be injected directly into the system and tested on sky. Furthermore, the use of relatively low cost components also offers significant advantages for the system hardware platform. However, the use of GPUs in an AO loop comes with drawbacks: the traditional way of offloading computation from CPU to GPUs - involving multiple copies and unacceptable overhead in kernel launching - is not well suited in a real time context. This last application requires the implementation of a solution enabling direct memory access (DMA) to the GPU memory from a third party device, bypassing the operating system. This allows this device to communicate directly with the real-time core of the simulation feeding it with the WFS camera pixel stream. We show that DMA between a custom FPGA-based frame-grabber and a computation unit (GPU, FPGA, or Coprocessor such as Xeon-phi) across PCIe allows us to get latencies compatible with what will be needed on ELTs. As a fine-grained synchronization mechanism is not yet made available by GPU vendors, we propose the use of memory polling to avoid interrupts handling and involvement of a CPU. Network and Vision protocols are handled by the FPGA-based Network Interface Card (NIC). We present the results we obtained on a complete AO loop using camera and deformable mirror simulators.

  12. ASEAN-China Governors/Mayors’ Dialogue Launched in Bo’ao, Hainan

    Institute of Scientific and Technical Information of China (English)

    Xu; Yan

    2015-01-01

    On March 27,2015,as an important program within the framework of the Bo’ao Forum for Asia(BFA)Annual Conference 2015,the ASEAN-China Governors/Mayors’Dialogue(ACGMD),organized by the CPAFFC and Hainan Provincial People’s Government,was launched with the participation of 16leaders from provinces and cities in China and ASEAN countries.They discussed"Local Coopera

  13. Gerontotecnologias para o ensino educativo direcionadas ao idoso: cuidado de enfermagem complexo

    OpenAIRE

    Hammerschmidt, Karina Silveira de Almeida

    2011-01-01

    Tese(doutorado) - Universidade Federal do Rio Grande, Programa de Pós-Graduação em Enfermagem, Escola de Enfermagem, 2011. A proposta de pesquisa teve como alicerce o cuidado de enfermagem complexo e a interdisciplinaridade, sendo o eixo central o ser humano idoso. Focalizando-se na gerontotecnologia educativa como instrumental para o repensar o ensino educativo no cuidado de enfermagem ao idoso. A tese é apresentada pelas assertivas: os docentes do curso de enfermagem apresentam-se potenc...

  14. Lesão liquenóide oral relacionada ao amálgama

    OpenAIRE

    Bernardes,Vanessa Fátima; Garcia,Bruna Gonçalves; Souto,Giovanna Ribeiro; Novaes-Junior,João Batista; Maria Cássia Ferreira de AGUIAR; Mesquita, Ricardo Alves

    2007-01-01

    A lesão liquenóide oral relacionada ao amálgama, condição rara na prática odontológica, constitui importante diagnóstico diferencial no grupo das leucoplasias orais. Relatam-se dois casos que apresentaram rápida resolução clínica após a substituição das restaurações de amálgama.

  15. Transtorno afetivo bipolar: perfil farmacoterapêutico e adesão ao medicamento

    Directory of Open Access Journals (Sweden)

    Adriana Inocenti Miasso

    2012-06-01

    Full Text Available Este estudo teve como objetivos verificar a adesão de portadores de transtorno afetivo bipolar (TAB à terapêutica medicamentosa e identificar possíveis causas de adesão e não adesão ao medicamento de acordo com o perfil farmacoterapêutico. Trata-se de estudo transversal, descritivo, realizado em Núcleo de Saúde Mental de um município do interior paulista. Participaram do estudo 101 pacientes com TAB. Para coleta dos dados, utilizou-se a entrevista estruturada e o teste de Morisky-Green e, para a análise dos mesmos, o programa Statistical Package for the Social Science. Os resultados mostraram que a maioria (63% dos sujeitos investigados não adere ao medicamento. Apesar de não ter ocorrido diferenças significativas entre o grupo de aderentes e não aderentes, para as variáveis investigadas, foi possível verificar a utilização de polifarmacoterapia e regimes terapêuticos complexos no tratamento do TAB. Permanece como desafio a implementação de estratégias que possam melhorar, na prática, a adesão de pacientes ao tratamento medicamentoso.

  16. Using the Fingerprinting Method to Customize RTLS Based on the AoA Ranging Technique

    Directory of Open Access Journals (Sweden)

    Bartosz Jachimczyk

    2016-06-01

    Full Text Available Real-time Locating Systems (RTLSs have the ability to precisely locate the position of things and people in real time. They are needed for security and emergency applications, but also for healthcare and home care appliances. The research aims for developing an analytical method to customize RTLSs, in order to improve localization performance in terms of precision. The proposed method is based on Angle of Arrival (AoA, a ranging technique and fingerprinting method along with an analytically defined uncertainty of AoA, and a localization uncertainty map. The presented solution includes three main concerns: geometry of indoor space, RTLS arrangement, and a statistical approach to localization precision of a pair of location sensors using an AoA signal. An evaluation of the implementation of the customized RTLS validates the analytical model of the fingerprinting map. The results of simulations and physical experiments verify the proposed method. The research confirms that the analytically established fingerprint map is the valid representation of RTLS’ performance in terms of precision. Furthermore, the research demonstrates an impact of workspace geometry and workspace layout onto the RTLS’ performance. Moreover, the studies show how the size and shape of a workspace and the placement of the calibration point affect the fingerprint map. Withal, the performance investigation defines the most effective arrangement of location sensors and its influence on localization precision.

  17. Escala de atitudes face ao lazer em adolescentes e jovens adultos

    Directory of Open Access Journals (Sweden)

    Teresa Freire

    2007-04-01

    Full Text Available Atualmente, o lazer e as atitudes face ao lazer têm sido alvo de interesse na investigação psicológica, concretamente no domínio da Psicologia Social. O objetivo do presente estudo foi adaptar a escala Leisure Attitude Scale de Raghb e Beard, construída para avaliar as atitudes face ao lazer em função das três componentes de atitude (cognitiva, afetiva e comportamental para a população de estudantes, adolescentes e jovens adultos portugueses. Participaram no estudo 462 sujeitos (57,8% do sexo feminino e 42,2% do sexo masculino, com uma média de idades de 19,97 (desvio padrão de 4,85. Os resultados obtidos confirmam a estrutura e as características psicométricas da versão original, refletindo valores largamente aceitáveis de fidelidade (consistência interna e validade (validade de conteúdo quer para a escala total quer para as várias sub-escalas (cognitiva, afetiva e comportamental. Conclui-se pela adequação da escala na medida das atitudes face ao lazer, no contexto português.

  18. Profilaxia das úlceras associadas ao estresse Prophylaxis of ulcers associated with stress

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Pompilio

    2010-06-01

    Full Text Available INTRODUÇÃO: As úlceras da mucosa gastroduodenal que aparecem após graves insultos têm sido chamadas de úlceras associadas ao estresse ou, mais genericamente, de "doença mucosa associada ao estresse" (DMAE. São conhecidas desde a Antiguidade e apesar de relacionarem-se com altos índices de morbidade e mortalidade, esquemas de profilaxia no intuito de minimizar seus riscos, só foram introduzidos recentemente. Os inibidores da bomba de prótons têm desempenhado papel importante nesse cenário. MÉTODOS: O presente artigo é uma breve revisão sobre seu uso dentro do contexto clínico da profilaxia do sangramento gastrointestinal relacionado ao estresse.INTRODUCTION: Gastroduodenal mucosal ulcers that appear after severe insults have been called ulcers associated with stress or, more generally, "mucosal disease associated with stress" (DMAE. Are known since antiquity and although linked with high morbidity and mortality, prevention schemes in order to minimize their risk, were introduced only recently. The proton pump inhibitors have played an important role in this scenario. METHODS: This article is a brief review of its use within the clinical context of prophylaxis of gastrointestinal bleeding due to stress.

  19. A URGÊNCIA NO ATENDIMENTO AO IDOSO: ESTUDO DE CASO

    Directory of Open Access Journals (Sweden)

    Ana Paula dos Santos Zepka

    2015-07-01

    Full Text Available O objetivo deste estudo foi caracterizar o perfil epidemiológico do idoso que busca consulta médica em Unidade de Pronto Atendimento. A amostra foi composta por 2273 pacientes, com idade acima de 60 anos atendidos em São José dos Campos, São Paulo, durante o ano de 2013. Os pacientes foram divididos em dez grupos de acordo com o diagnóstico, baseado na CID-10. As mulheres representaram a maioria dos pacientes (59%. Do total de idosos, cerca de 24% foram diagnosticados como grupo X (doenças variadas e não graves, demonstrando que um quarto da amostra foi de pacientes não urgentes, os quais poderiam ter sido consultados em outros serviços de saúde, ao invés do Pronto Atendimento. O conhecimento sobre o idoso que busca o serviço de urgência é fundamental para a orientação desse usuário ao local mais adequado para o tratamento. Com o aumento da expectativa de vida, é imprescindível a implementação de políticas públicas direcionadas ao idoso. O cuidado com esse paciente é incipiente, pois os serviços de saúde ainda necessitam ser estruturados de acordo com as necessidades específicas desse público.

  20. Simultaneous Nitrogen and Phosphorus Removal by Denitrifying Dephosphatation in a (AO)2 Sequencing Batch Reactor

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yan-ping; PENG Yong-zhen; WANG Shu-ying; WANG Shao-po

    2005-01-01

    A 24 L working volume reactor was used for the research on simultaneous phosphorus (P) and nitrogen (N) removal by denitrifying dephosphatation in an anaerobic-oxid-anoxic-oxid sequencing batch reactor ((AO)2SBR) system. The durations of each phase are: anaerobic 1.5 h, aerobic 2.5 h, anoxic 1.5 h, post-aerobic 0.5 h, settling 1.0 h, fill 0.5 h. The successful removal of nitrogen and phosphorus is achieved in a stable (AO)2SBR. The effluent P concentrations is below 1 mg/L, and the COD,TN and P average removal efficiency is 88.9%, 77.5% and 88.7%, respectively. The batch experiment results show that the durations of aerobic and anoxic phase influence the P removal efficiency. Some feature points are found on the DO, ORP and pH curves to demonstrate the complete of phosphate release and phosphate uptake. These feature points can be used for the control of (AO)2 SBR.