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Sample records for ao lenho mole

  1. Evidência molecular da ocorrência de um fitoplasma associado ao lenho mole da macieira

    OpenAIRE

    Ribeiro,Luiz Fernando Caldeira; Bedendo,Ivan Paulo; Sanhueza,Rosa Maria Valdebenito

    2007-01-01

    O lenho mole da macieira é uma doença relevante em diversas partes do mundo. Sintomas típicos desta doença têm sido observados em pomares instalados em estados do sul do território brasileiro desde a década de oitenta. Enxertia tem revelado a natureza infecciosa da doença e a observação de corpúsculos filamentosos no floema tem evidenciado possível associação com fitoplasma. No presente trabalho plantas com sintomas de lenho mole foram coletadas em pomar comercial, visando demonstrar a presen...

  2. Evidência molecular da ocorrência de um fitoplasma associado ao lenho mole da macieira Molecular evidence for an association of a phytoplasma with apple rubbery wood

    Directory of Open Access Journals (Sweden)

    Luiz Fernando Caldeira Ribeiro

    2007-03-01

    Full Text Available O lenho mole da macieira é uma doença relevante em diversas partes do mundo. Sintomas típicos desta doença têm sido observados em pomares instalados em estados do sul do território brasileiro desde a década de oitenta. Enxertia tem revelado a natureza infecciosa da doença e a observação de corpúsculos filamentosos no floema tem evidenciado possível associação com fitoplasma. No presente trabalho plantas com sintomas de lenho mole foram coletadas em pomar comercial, visando demonstrar a presença de fitoplasma em tecido doente, bem como identificar molecularmente este fitoplasma. Através do emprego de duplo PCR com iniciadores universais R16mF2/R1 e R16F2n/R2, fitoplasma foi consistentemente detectado em plantas sintomáticas. A identificação conduzida com duplo PCR usando-se iniciadores específicos R16(IIIF2/R demonstrou que o fitoplasma detectado pertencia ao grupo 16SrIII. Análises de RFLP conduzidas com as endonucleases AluI, KpnI, HinfI, HpaII, MseI, RsaI e SauIIIA confirmaram que o fitoplasma era um representante típico do grupo 16SrIII. A detecção e identificação molecular se constitui numa forte evidência que um fitoplasma está associado ao lenho mole da macieira no Brasil, complementando os trabalhos realizados anteriormente com transmissão por enxertia e observação por microscopia eletrônica .Apple rubbery wood is an important disease occurring worldwide. Typical symptoms have been observed since 80' decade in orchards located in the South part of Brazil. In previous studies, grafting has evidenciated that the disease had infeccious etiology and visualization of filamentous bodies inside phloem had indicated that a phytoplasma could be associated with the disease. In the present study, plants with symptoms of rubbery wood were sampled in a commercial orchard in order to demonstrate the presence of phytoplasma in infected tissue and to identify molecularly that the organism. Using nested PCR with universal

  3. The mole

    CERN Multimedia

    CERN PhotoLab

    1974-01-01

    The mole continued its way around the SPS ring ... and on 31 July emerged where it started from, having completed its 7 kilometre journey without ever veering more than a couple of centimetres from its schedule path.

  4. Estudo da variação da resistência ao cisalhamento do resíduo solido urbano devido a presença de plástico mole

    OpenAIRE

    Gawlik, Francismara Martins

    2014-01-01

    A influencia do plástico mole na resistência ao cisalhamento do resíduo solido urbano (RSU) vem sendo alvo constante de estudo em virtude da sua enorme relevância nesta questão. No entanto trata-se de um tema complexo, visto que, são muitas as variáveis relacionadas ao problema, tais como, a quantidade de plástico, influencia do chorume em contanto com o plástico, inclinação do plástico em relação aos esforços normais e cisalhantes, resistência à tração do plástico, questões relacionadas à mo...

  5. Variação anatômica no lenho de Astronium lecointei Ducke

    Directory of Open Access Journals (Sweden)

    Luiz Eduardo de Lima Melo

    Full Text Available Com o objetivo de avaliar a variação dos elementos celulares de Astronium lecointei Ducke no sentido medula-câmbio, analisaram-se amostras de madeira de três regiões radiais da árvore, a 1,30 m do solo: 0% (próximo à medula, 50% (região intermediária e 100% (próximo ao câmbio vascular. A metodologia aplicada no trabalho foi aquela tradicionalmente recomendada para estudos em anatomia de madeira, seguindo orientação do IAWA Committee. Observou-se que alguns elementos anatômicos apresentaram tendências de variação definidas e estatisticamente significativas: frequência, diâmetro e comprimento dos elementos de vaso, altura dos raios em micrômetros e comprimento das fibras. Observou-se também maior obstrução dos poros por tilas próximo à medula e na região intermediária. A tendência de estabilização das dimensões dessas estruturas pode estar indicando a formação de madeira adulta a partir da região intermediária analisada no lenho da espécie.

  6. Anatomia do lenho de cinco espécies comercializadas como 'Sucupira'

    Directory of Open Access Journals (Sweden)

    Warley Felício Soares

    Full Text Available A análise comparativa da anatomia do lenho das espécies Bowdichia nitida Spruce ex Benth, Diplotropis brasiliensis (Tul. Benth., Diplotropis martiusii Benth., Diplotropis purpurea (Rich. Amshoff e Diplotropis racemosa (Hoehne Amshoff é descrita no presente trabalho, visando a determinar as principais características anatômicas necessárias à distinção dessas espécies. Qualitativamente, as características anatômicas mais importantes para a diferenciação do lenho dessas espécies foram: camadas de crescimento, estratificação dos raios, arranjo dos vasos e fileira marginal dos raios. Quantitativamente, a análise estatística mostrou que os parâmetros anatômicos mais importantes no agrupamento das espécies foram: diâmetro dos elementos de vasos, largura dos raios em micrometros, comprimento, espessura da parede e largura das fibras. Verificou-se que a anatomia do lenho pode ser utilizada como importante subsídio na identificação das espécies estudadas.

  7. Anatomia do lenho de Raulinoa echinata R.S.Cowan (Rutaceae uma espécie endêmica. Wood anatomy of Raulinoa echinata R.S.Cowan (Rutaceae an endemic species.

    Directory of Open Access Journals (Sweden)

    Adriano Antonio DAROSCI

    2012-12-01

    Full Text Available A anatomia vegetal é útil para a caracterização das espécies e do ambiente em que estas vivem. Muitas espécies respondem a algumas características ambientais com alterações na anatomia do lenho, por exemplo, na quantidade de vasos e raios, o que contribui para a sobrevivência no ambiente. Outras respostas anatômicas do lenho, devido às condições fisiológicas-estruturais presentes em diferentes regiões do caule, também são comuns. O presente estudo analisou a anatomia do lenho de Raulinoa echinata, espécie endêmica da mata ciliar do Vale do Itajaí, a fim de buscar alterações do lenho em resposta a algumas características ambientais, além daquelas provocadas pelas diferentes regiões do caule. Para tanto, amostras do lenho foram coletadas em duas regiões: próxima do solo e à altura do peito (1,30 m. Possíveis respostas de R. echinata ao ambiente ripário foram observadas no comprimento e na frequência das fibras, na largura dos raios e no comprimento e na frequência total de vasos, com diferenças significativas, entre as duas regiões, apenas em características quantitativas. A espécie apresentou, ainda, vasos múltiplos tangenciais e em cacho, características até então não descritas para Rutaceae. Não foram vistos na espécie canais traumáticos, característica comum para Rutaceae. Plant anatomy is useful for the characterization of species and their associated environment. Many species, because of habitat features, can show anatomical alterations of wood that permit their survival in the environment, quantity of vessels and rays, for example. Other anatomical alterations caused by structural-physiological conditions present in different regions of the stem are also common. In this study, the anatomy of Raulinoa echinata wood, an endemic riparian forest species of Itajaí Valley, was analyzed in order to identify alterations in wood because of specific habitat features, as well as adaptations in different

  8. Mole gun injury.

    Science.gov (United States)

    Pistré, V; Rezzouk, J

    2013-09-01

    A mole gun is a weapon, which is used to trap and kill moles. This report provides an overview of the state of knowledge of mole gun injuries, comparable to blast injuries caused by fireworks, explosive or gunshot. Over a 2-year period, the authors reported their experience with ten hand injuries caused by mole gun. Radial side of the hand was often concerned, particularly the thumb. The authors explain their choices in the management of such lesions. Surgery was performed primarily and a large debridement currently seemed to offer the best outcome for the patient. Blast, crush, burns and lacerations may explain the higher rate of amputation to the digits. A long period of physiotherapy, specifically of the hand, was needed before the patient could return to work. This ballistic hand trauma encountered by surgeons requires knowledge and understanding of these injuries. It should be in accordance with firearms law because of severe injuries encountered and possible lethal wounds. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  9. Trend of Complete Hydatidiform Mole

    Directory of Open Access Journals (Sweden)

    K Thapa

    2010-03-01

    Full Text Available INTRODUCTION: Complete Hydatidiform mole is one of the most frequent abnormal pregnancies. This review studies the trend of complete mole in Paropakar Maternity and Women's hospital and clinical ability to detect it. METHODS: This is a retrospective study of 504 cases of complete hydatidiform mole recorded at Paropakar maternity and women's hospital, Kathmandu, during 2058-2065 B.S. Medical records were reviewed and incidence, clinical presentation and method of diagnosis were studied. RESULTS: During the study period, there were 13,9117 births and 504 complete moles, 12 partial moles, 48 persistent gestational tumours, six choriocarcinoma and four invasive moles recorded in the hospital. The incidence of complete mole was one per 276 births. It was prevalent among women younger than 29 years (80% and among the primigravidae (36.7%. More than 90% women presented in the first half of their pregnancy and vaginal bleeding was the main complaint (68.3%. Suction evacuation, dilation and evacuation followed by sharp curettage and abdominal hysterectomy were performed in 80.6%, 17.6% and 1.2% of the women respectively. Persistent mole and choriocarcinoma developed in 9.5% and 0.4% respectively. CONCLUSIONS: Complete mole has the highest incidence. It affects mostly younger women and presents with vaginal bleeding most of the time, usually in the first half of their pregnancy. Keywords: complete hydatidiform mole, gestational trophoblastic disease, persistent gestational tumours.

  10. Mole sieve cycle optimization

    Energy Technology Data Exchange (ETDEWEB)

    Croteau, P. [Petro-Canada Oil and Gas, Inc., Calgary, AB (Canada)

    2004-07-01

    Maturing gas basins and declining reservoirs have prompted the search for energy and cost reduction initiatives to maintain operation sustainability. This paper discusses the development of an algorithm to improve the efficiency of the Brazeau mole sieve dehydration system. Details of the operating unit and an outline of the system were provided, as well as an operator interface. The main objectives of the research were to reduce the number of corresponding regeneration cycles; reduce annual energy requirements; and extend the life of the dessicant. Reductions were anticipated at 35 per cent, leading towards fuel savings of $45,000 a year, and carbon dioxide reductions of 670 tonnes a year. However, significant barriers were also noted, including operations, maintenance and equipment replacement issues. Equations of state were used to estimate vapor pressure or water content in gases. Approximate water content was measured with gas flow rate. The resulting algorithm was implemented using a basic best fit curve generated for one variable with a correction factor applied as a second variable. Data books were provided to present water content of hydrocarbon gas, with implementation procedures. Benefits of the new system included longer service life and reduced chemical and outside service costs, and reduced lost production during sieve changes. Additional benefits included enhanced distributed computing system (DCS) interfaces within plant operations; extension of the adsorption cycle via the functionality of the algorithm; and the fact that during a plant upset, the algorithm froze cycle sequencing and eliminated the possibility of switching the beds and repeating regeneration. The improved system resulted in savings of $87,000. tabs, figs.

  11. Histamine combined with melphalan in isolated limb perfusion for the treatment of locally advanced soft tissue sarcomas: preclinical studies in rats Histamina combinada ao melfalano na perfusão de membro isolado para o tratamento de sarcomas de partes moles localmente avançado: estudos pré-clínicos em ratos

    Directory of Open Access Journals (Sweden)

    Flavia Brunstein

    2005-08-01

    Full Text Available PURPOSE: To evaluate the potential benefit of histamine combined with melphalan in the isolated limb perfusion (ILP as an alternative to TNF-alfa and melphalan combination, for the treatment of irressectable soft tissue sarcomas of the limbs in Brown Norway (BN rats. METHODS: 20 BN rats had small fragments of syngeneic BN-175 fibosarcoma inserted on the right hind limb. In 7-10 days the tumor reached a median diameter of 12-15 mm and they were randomly divided in four groups (sham, melphalan, histamine and escalating doses of histamine combined to melphalan being submitted to experimental ILP for 30 minutes. Tumors were measured daily with a caliper and the volume was calculated. RESULTS: Response curves showed a significant effect of the combination of histamine 200 mg/mL with melphalan, with 66% overall response, including 33% complete responses (pOBJETIVO: Avaliar o potencial benéfico da histamina combinada ao melfalano, na perfusão de membro isolado (PMI, como alternativa à combinação TNF-alfa mais melfalano, no tratamento de sarcomas de partes moles irressecaveis em extremidades, em ratos de linhagem Brown Norway (BN. MÉTODOS: 20 ratos BN foram submetidos a implantação de fragmentos de fibrosarcoma singênico BN-175 na pata traseira direita. Em cerca de 7-10 dias o tumor atingiu um diâmetro médio de 12-15 mm e foram aleatóriamente divididos em quatro grupos (controle, melfalano, histamina em doses progessivas combinada ao melfalano e histamina sendo submetidos a PMI experimental por 30 minutos. Os tumores foram então medidos diariamente com o uso de paquímetro e o volume tumoral calculado. RESULTADOS: As curvas de resposta mostram um efeito significativo da combinação de Histamina na concentração de 200 mg/mL ao melfalano, com 66% de resposta global incluindo 33% de respostas completas (p < 0.01. Não houve efeitos colaterais sistêmicos e localmente apenas edema leve e transitório nos animais tratados com histamine

  12. Moles

    Science.gov (United States)

    ... This can be done with a split thickness skin graft from some other normal area of the body. ... several reported cases of melanoma developing underneath a skin graft from nevus cells left behind. Occasionally, thickened scars ...

  13. Anatomia comparada do lenho de Piptadenia gonoacantha (Mart.) J.F.Macbr. em dois tipos de vegetação

    OpenAIRE

    Longui, Eduardo Luiz; Bufolo, Alaor; Aguiar, Osny Tadeu de; Lima, Israel Luiz de; Florsheim, Sandra Monteiro Borges

    2009-01-01

    A anatomia ecológica tem como objetivo correlacionar as estruturas morfológicas e anatômicas das plantas com dados climáticos, geográficos e do solo. Nesse contexto, avaliaram-se as diferenças qualitativas e quantitativas no lenho de árvores de pau-jacaré -Piptadenia gonoacantha procedentes do Parque Estadual Cantareira (PEC) e da Estação Ecológica de Caetetus (EEC), áreas respectivamente de Floresta ombrófila densa e Floresta estacional semidecidual. Os parâmetros anatômicos foram analisados...

  14. Moles and Mole Control on British Farms, Amenities and Gardens after Strychnine Withdrawal

    Directory of Open Access Journals (Sweden)

    Sandra E. Baker

    2016-06-01

    Full Text Available Moles are considered pests in Britain, but this issue has been little studied. Lower welfare standards have been tolerated for moles than for most other managed wild mammal species, as use of both the controversial poison, strychnine, and unregulated traps have been permitted. Strychnine was withdrawn in 2006 and there were fears that mole populations would increase as a result. In 2007, we conducted a comprehensive, nationwide survey of land manager perceptions, opinions and behaviour regarding moles and mole control on farms, amenities and domestic gardens in Britain. We surveyed 2150 land managers (achieving a 59% response rate and ground-truthed 29 responses. Moles were reported to be present on most farms and amenities, and 13% of gardens, and were more common in lighter soils. Where present, moles were usually considered pests, this being more likely in Wales, Scotland and northern England, on livestock and mixed farms, and on large, high-value amenities, e.g., racecourses and golf courses. Mole control followed similar patterns to mole presence. More control may occur than is economically, and therefore potentially ethically, justified. Control should be more carefully considered and, where necessary, more effectively targeted. Kill-trapping was the favoured recent and future method on farms and amenities, even if strychnine was to be reintroduced; however, because mole traps are currently unregulated, some might not meet current welfare standards if tested. We found no evidence for an increase in moles since a farm questionnaire survey conducted in 1992; this could have wider implications for future wildlife management policy changes.

  15. Anatomia comparada do lenho de Xylopia aromatica (Lam.) Mart. em áreas de cerrado e de plantação de Pinus elliottii Engelm.

    OpenAIRE

    Luchi, Agnes E.; Silva, Luiz C. Pereira; Moraes, Marcelo A.

    2005-01-01

    A estrutura anatômica do lenho de Xylopia aromatica (Lam.) Mart. foi analisada pela observação do caule e da raiz de espécimes provenientes de duas áreas de cerrado, uma com vegetação característica e outra, em que a vegetação original foi substituída por monocultura de Pinus elliotti Engelm. As duas áreas situam-se na Reserva Biológica e Estação Experimental de Moji-Guaçu. As amostras foram coletadas pelo método não destrutivo. Os dados quantitativos das características analisadas foram obti...

  16. Caracterização do lenho e variação radial de Pittosporum undulatum Vent. (pau-incenso)

    OpenAIRE

    Longui, Eduardo Luiz; Romeiro, Diego; Silva, Morgana Tramontini da; Ribeiro, Ariane; Gouveia, Tatiana Cestini; Lima, Israel Luiz de; Florsheim, Sandra Monteiro Borges

    2011-01-01

    Estudou-se o lenho de Pittosporum undulatum do Parque Estadual Alberto Löfgren, objetivando-se sua caracterização, investigar a variação radial da anatomia e densidade básica, suas correlações e discutir os resultados em um contexto ecológico. Empregaram-se as metodologias usuais para anatomia e densidade da madeira. As características anatômicas corroboram com o descrito para o gênero, ressalta-se a presença de camadas de crescimento, não mencionada na literatura. O diâmetro das fibras não a...

  17. Subsurface Sampling and Sensing Using Burrowing Moles

    Science.gov (United States)

    Stoker, C. R.; Richter, L.; Smith, W. H.

    2004-01-01

    Finding evidence for life on Mars will likely require accessing the subsurface since the Martian surface is both hostile to life and to preservation of biosignatures due to the cold dry conditions, the strong W environment, and the presence of strong oxidants. Systems are needed to probe beneath the sun and oxidant baked surface of Mars and return samples to the surface for analysis or to bring the instrument sensing underground. Recognizing this need, the European Space Agency incorporated a small subsurface penetrometer or Mole onto the Beagle 2 Mars lander. Had the 2003 landing been successful, the Mole would have collected samples from 1-1.5 m depth and delivered them to an organic analysis instrument on the surface. The de- vice called the Planetary Underground Tool (PLUTO), also measured soil mechanical and thermophysical properties. Constrained by the small mass and volume allowance of the Beagle lander, the PLUTO mole was a slender cylinder only 2 cm diameter and 28 cm long equipped with a small sampling device designed to collect samples and bring them to the surface for analysis by other instrument. The mass of the entire system including deployment mechanism and tether was 1/2 kg. sensor package underground to make in situ measurements. The Mars Underground Mole (MUM) is a larger Mole based on the PLUTO design but incorporating light collection optics that interface to a fiber optic cable in the tether that transmits light to a combined stimulated emission Raman Spectrometer and Short Wave Infrared (SWIR) reflectance Spectrometer with sensitivity from 0.7 to 2.5 micrometers. This instrument is called the Dual Spectral Sensor and uses a Digital Array Scanning Interferometer as the sensor technology, a type of fourier transform interferometer that uses fixed element prisms and thus is highly rugged compared to a Michaelson interferometer. Due to the size limitations of an on-Mole instrument compartment, and the availability of a tether, the sensor head

  18. Reproductive features of the eastern mole (Scalopus aquaticus) and star-nose mole (Condylura cristata).

    Science.gov (United States)

    Bedford, J M; Mock, O B; Nagdas, S K; Winfrey, V P; Olson, G E

    1999-11-01

    Since moles are closely related to shrews, the gametes and reproductive tracts of the star-nose mole (Condylura cristata) and the eastern mole (Scalopus aquaticus) were examined to gain further insight into unusual reproductive traits of the Soricidae. Moles display many of these soricid traits, but with some important differences. The cumulus oophorus of Scalopus, ovulated about 16 h after hCG injection, was largely dispersed by hyaluronidase and, though quite dense, was nevertheless more similar to that of higher mammals than to the compact 'ball of the soricid cumulus. Within the female tract in these moles, approximately 85% of the length of the oviduct comprises a narrow ampulla with numerous differentiated crypts that, in shrews, house spermatozoa. However, in contrast to shrews, moles produce considerably larger numbers of spermatozoa, which challenges the proposal that, in shrews, oviductal sperm crypts specifically permit lower sperm production by the males. In the sperm head of these two moles, the acrosome displays the long rostrum that is typical of other Insectivora, and the perforatorium has the barbs by which soricid spermatozoa probably bind to the zona pellucida. Perhaps allied to this, immunoblots indicated that the immunoreactive acrosomal matrix of Scalopus spermatozoa is simpler than the polypeptide complex of the bovine and hamster acrosomal matrix.

  19. The Origin of the Mole Concept

    Science.gov (United States)

    Jensen, William B.

    2004-01-01

    German Chemist, August Wilhelm Hofmann first introduced the term "molar" (from the Latin moles, meaning "a large mass") into chemistry, around 1865. The particular use of the term molar gained currency in the physics literature, where it was in common use at least through the 1940s.

  20. psychrometry: from partial pressures to mole fractions

    African Journals Online (AJOL)

    ES Obe

    1980-03-01

    Mar 1, 1980 ... as an ideal gas mixture. Partial pressures then become identical: to mole fractions and sets of psychometric parameters result from rather elementary thermodynamic relations. Search for more accurate data has long led to the realization that neither dry air nor pure water vapour behaves like an ideal gas,.

  1. CT diagnosis of invasive hydatidiform mole

    International Nuclear Information System (INIS)

    Lu Mingquan; Jiang Lina; Li Xianxing; Wang Peijun

    2000-01-01

    Objective: To investigate the CT features and the diagnostic value in invasive mole. Methods: Eleven cases (age, 30 to 53 years, mean 40 years) with invasive mole proved by operation and pathology were studied, including 3 cases during peri-menopausal period of eight months, ten months and twelve months respectively. All cases were scanned by CT and enhanced study was done. Results: All cases showed enlargement of uterus of varying degrees. On CT, the density of uterine cavity was similar to that of water, intermingled with spotty patchy and hazy circular isodense shadows. High density stripe-like bleeding foci were found in 3 cases. The outline of uterine cavity was locally disrupted in all case and the muscle layer was thickened in 3 cases. On enhanced CT scan, the lesions in the uterine cavity appeared markedly hyperdense, just like 'the crater' and the 'mole sign'. The muscle layers at the site of disruption showed inhomogeneous enhancement after contrast injection. Conclusion: The CT features of invasive mole are characteristic, with important diagnostic value, especially for those during peri-menopausal period

  2. Social structure predicts genital morphology in African mole-rats.

    Directory of Open Access Journals (Sweden)

    Marianne L Seney

    2009-10-01

    Full Text Available African mole-rats (Bathyergidae, Rodentia exhibit a wide range of social structures, from solitary to eusocial. We previously found a lack of sex differences in the external genitalia and morphology of the perineal muscles associated with the phallus in the eusocial naked mole-rat. This was quite surprising, as the external genitalia and perineal muscles are sexually dimorphic in all other mammals examined. We hypothesized that the lack of sex differences in naked mole-rats might be related to their unusual social structure.We compared the genitalia and perineal muscles in three African mole-rat species: the naked mole-rat, the solitary silvery mole-rat, and the Damaraland mole-rat, a species considered to be eusocial, but with less reproductive skew than naked mole-rats. Our findings support a relationship between social structure, mating system, and sexual differentiation. Naked mole-rats lack sex differences in genitalia and perineal morphology, silvery mole-rats exhibit sex differences, and Damaraland mole-rats are intermediate.The lack of sex differences in naked mole-rats is not an attribute of all African mole-rats, but appears to have evolved in relation to their unusual social structure and reproductive biology.

  3. Social structure predicts genital morphology in African mole-rats.

    Science.gov (United States)

    Seney, Marianne L; Kelly, Diane A; Goldman, Bruce D; Sumbera, Radim; Forger, Nancy G

    2009-10-15

    African mole-rats (Bathyergidae, Rodentia) exhibit a wide range of social structures, from solitary to eusocial. We previously found a lack of sex differences in the external genitalia and morphology of the perineal muscles associated with the phallus in the eusocial naked mole-rat. This was quite surprising, as the external genitalia and perineal muscles are sexually dimorphic in all other mammals examined. We hypothesized that the lack of sex differences in naked mole-rats might be related to their unusual social structure. We compared the genitalia and perineal muscles in three African mole-rat species: the naked mole-rat, the solitary silvery mole-rat, and the Damaraland mole-rat, a species considered to be eusocial, but with less reproductive skew than naked mole-rats. Our findings support a relationship between social structure, mating system, and sexual differentiation. Naked mole-rats lack sex differences in genitalia and perineal morphology, silvery mole-rats exhibit sex differences, and Damaraland mole-rats are intermediate. The lack of sex differences in naked mole-rats is not an attribute of all African mole-rats, but appears to have evolved in relation to their unusual social structure and reproductive biology.

  4. Definition of the mole (IUPAC Recommendation 2017)

    OpenAIRE

    Marquardt, Roberto; Meija, Juris; Mester, Zoltán; Towns, Marcy; Weir, Ron; Davis, Richard; Stohner, Jürgen

    2018-01-01

    In 2011 the General Conference on Weights and Measures (CGPM) noted the intention of the International Committee for Weights and Measures (CIPM) to revise the entire International System of Units (SI) by linking all seven base units to seven fundamental physical constants. Of particular interest to chemists, new definitions for the kilogram and the mole have been proposed. A recent IUPAC Technical Report discussed these new definitions in relation to immediate consequences for the chemical co...

  5. Prevalence of Toxoplasma gondii in common moles (Talpa europaea)

    NARCIS (Netherlands)

    Krijger, I.M.; Cornelissen, J.B.W.J.; Wisselink, H.J.; Meerburg, B.G.

    2014-01-01

    Background The prevalence of Toxoplasma gondii in common moles, Talpa europaea, was investigated in order to determine whether moles can serve as an indicator species for T. gondii infections in livestock. Findings In total, 86 moles were caught from 25 different sites in the Netherlands. Five

  6. Caracterização e descrição da estrutura anatômica do lenho de seis espécies arbóreas com potencial medicinal Characterization and description of wood anatomical structure in six tree species with medicinal potential

    Directory of Open Access Journals (Sweden)

    G. Cury

    2011-01-01

    Full Text Available A caracterização morfoanatômica e a correta identificação das plantas medicinais são fundamentais para o controle de qualidade dos fitoterápicos. No presente trabalho é caracterizada a anatomia do lenho visando à identificação de seis espécies arbóreas com potencial medicinal. As amostras do lenho foram extraídas por método não destrutivo do tronco das árvores das espécies desenvolvidas em áreas de preservação. Em laboratório as amostras do lenho foram processadas para análises microscópicas do lenho, aplicando as metodologias para estudos de anatomia. Os resultados mostraram que o parênquima axial foi o parâmetro anatômico mais efetivo para a distinção das espécies através da análise do seu lenho. No trabalho discute-se a importância da aplicação da anatomia do lenho como ferramenta na identificação de espécies arbóreas medicinais.The morphoanatomical characterization and the correct identification of medicinal plants are fundamental for the quality control of phytotherapics. In the present work, wood anatomy was characterized in order to identify six tree species with medicinal potential. Wood samples were collected through a non-destructive method from the trunk of trees developed in conservation areas. In the laboratory, wood samples were processed for microscopic analysis by applying the methods for anatomical studies. Axial parenchyma was the most effective anatomical parameter to distinguish species through wood analysis. In this work, the importance of wood anatomy as a tool for the identification of medicinal tree species was discussed.

  7. Variação intraespecífica do lenho de Eugenia uniflora L. em duas diferentes fitofisionomias do complexo vegetacional atlântico

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    Priscila Alves Marques

    Full Text Available Myrtaceae está entre as principais famílias lenhosas da Floresta Atlântica, destacando-se Eugenia L. como o gênero de maior riqueza de espécies na família. Eugenia uniflora L. apresenta grande representatividade em áreas de restinga, seu ambiente natural, e é amplamente cultivada em outras regiões em função da sua importância econômica. Este estudo investigou a anatomia do lenho de E. uniflora, crescendo em duas fitofisionomias do complexo vegetacional atlântico no Estado do Rio de Janeiro. Dezesseis parâmetros anatômicos foram analisados e revelaram que os elementos celulares dos indivíduos de restinga apresentam maior frequência e são mais curtos e mais largos, e os raios mais baixos e largos do que os dos indivíduos crescendo na Floresta Ombrófila Densa. Os resultados mostraram como as condições ambientais influenciam a estrutura anatômica da madeira e indicam variações intraespecíficas da espécie e de seus mecanismos de adaptação e de sobrevivência no complexo Mata Atlântica.

  8. Ultrasonographic findings of hydatidiform mole and missed abortion

    International Nuclear Information System (INIS)

    Yun, Kwang Myeong; Lee, Yeong Hwan; Chung, Hye Kyeong; Chung, Duck Soo; Kim, Ok Dong

    1990-01-01

    To establish the sonographic characteristics of the hydatidiform mole and the missed abortion with placental degeneration, we have retrospectively analyzed 12 cases of complete mole, 10 cases of partial mole, and 10 cases of missed abortion with placental hydropic degeneration, collected at Taegu Catholic General Hospital, from January 1986 to December 1989. The results were as follows : 1. Of 12 cases of complete mole, all demonstrated diffuse intrauterine vesicular pattern of internal echo without a gestational sac. Two cases were recurred after D and E. 2. The partial mole was characterized by focal (70%) or diffuse (20%) distribution of hydatidiform placental change and a gestational sac (100%) with or without a macerated fetus. But the striking hydatidiform placental change was not present in one cases of partial mole. 3. The uterus was larger for dates in 9 cases (90%) of complete mole, but smaller for dates in 7 cases (70%) of partial mole. 4. The missed abortion with placental hydropic degeneration was indistinguished from a partial mole due to their similar sonographic appearance : focal or diffuse cystic change of a placenta, a distorted gestational sac with or without a fetus, and a smaller uterus for dates. On conclusion, the complete mole could be easily distinguished from a partial mole or a missed abortion by sonography : a gestational sac or an area of noncystic placenta was not identified in a complete mole. The partial mole was indistinguished from a missed abortion, but if there is the suspicion of trophoblastic proliferation, such as a convex placental surface or a larger uterus for dates, then the diagnosis is probably a partial mole rather than a missed abortion

  9. Instrumented Moles for Planetary Subsurface Regolith Studies

    Science.gov (United States)

    Richter, L. O.; Coste, P. A.; Grzesik, A.; Knollenberg, J.; Magnani, P.; Nadalini, R.; Re, E.; Romstedt, J.; Sohl, F.; Spohn, T.

    2006-12-01

    Soil-like materials, or regolith, on solar system objects provide a record of physical and/or chemical weathering processes on the object in question and as such possess significant scientific relevance for study by landed planetary missions. In the case of Mars, a complex interplay has been at work between impact gardening, aeolian as well as possibly fluvial processes. This resulted in regolith that is texturally as well as compositionally layered as hinted at by results from the Mars Exploration Rover (MER) missions which are capable of accessing shallow subsurface soils by wheel trenching. Significant subsurface soil access on Mars, i.e. to depths of a meter or more, remains to be accomplished on future missions. This has been one of the objectives of the unsuccessful Beagle 2 landed element of the ESA Mars Express mission having been equipped with the Planetary Underground Tool (PLUTO) subsurface soil sampling Mole system capable of self-penetration into regolith due to an internal electro-mechanical hammering mechanism. This lightweight device of less than 900 g mass was designed to repeatedly obtain and deliver to the lander regolith samples from depths down to 2 m which would have been analysed for organic matter and, specifically, organic carbon from potential extinct microbial activity. With funding from the ESA technology programme, an evolved Mole system - the Instrumented Mole System (IMS) - has now been developed to a readiness level of TRL 6. The IMS is to serve as a carrier for in situ instruments for measurements in planetary subsurface soils. This could complement or even eliminate the need to recover samples to the surface. The Engineering Model hardware having been developed within this effort is designed for accommodating a geophysical instrument package (Heat Flow and Physical Properties Package, HP3) that would be capable of measuring regolith physical properties and planetary heat flow. The chosen design encompasses a two-body Mole

  10. Investigação da presença de óleo essencial em Eucalyptus smithii R.T. Baker por meio da anatomia de seu lenho e casca.

    Directory of Open Access Journals (Sweden)

    Fernando José Fabrowski

    2010-08-01

    Full Text Available O material utilizado no presente estudo foi proveniente de 15 árvores de Eucalyptus smithii R.T. Baker cultivadas no município de Colombo - PR, em campo experimental da Embrapa Florestas, plantadas em 1988. Caracterizou-se anatomicamente o lenho e a casca por meio de lâminas preparadas no Laboratório de Anatomia e Identificação de Madeiras do IPT e Laboratório de Anatomia da Madeira, do Centro de Ciências Florestais e da Madeira, da Universidade Federal do Paraná. Para a extração do óleo essencial, a madeira foi transformada em serragem e a casca desfibrada manualmente. Para a determinação do rendimento e análise do óleo essencial, foram efetuadas destilações conforme ABNT no Laboratório de Fitoquímica do Departamento de Farmácia da Universidade Federal do Paraná. Os rendimentos do óleo essencial foram dados em volume (ml de óleo essencial por massa (g de material. O Eucalyptus smithii apresenta estrutura anatômica homogênea do lenho e da casca. Na casca, observou-se a presença de cavidades secretoras; no entanto, no lenho, não foi encontrada nenhuma estrutura semelhante, apenas bolsas de quino, sendo desprezível a quantidade de óleo essencial presente na madeira. O óleo essencial da casca apresentou características físico-químicas fora das especificações para óleos essenciais de eucaliptos ricos em 1,8-cineol, não sendo economicamente viável para a exploração desse componente.

  11. MOLES3: Implementing an ISO standards driven data catalogue

    Directory of Open Access Journals (Sweden)

    Graham A Parton

    2015-02-01

    Full Text Available ISO19156 Observations and Measurements (O&M provides a standardised framework for organising information about the collection of information about the environment.  Here we describe the implementation of a specialisation of O&M for environmental data, the Metadata Objects for Linking Environmental Sciences (MOLES3.MOLES3 provides support for organising information about data, and for user navigation around data holdings. The implementation described here, “CEDA-MOLES”, also supports data management functions for the Centre for Environmental Data Archival, CEDA. The previous iteration of MOLES (MOLES2 saw active use over five years, being replaced by CEDA-MOLES in late 2014. During that period important lessons were learnt both about the information needed, as well as how to design and maintain the necessary information systems. In this paper we review the problems encountered in MOLES2; how and why CEDA-MOLES was developed and engineered; the migration of information holdings from MOLES2 to CEDA-MOLES; and, finally, provide an early assessment of MOLES3 (as implemented in CEDA-MOLES and its limitations.Key drivers for the MOLES3 development included the necessity for improved data provenance, for further structured information to support ISO19115 discovery metadata  export (for EU INSPIRE compliance, and to provide appropriate fixed landing pages for Digital Object Identifiers (DOIs in the presence of evolving datasets. Key lessons learned included the importance of minimising information structure in free text fields, and the necessity to support as much agility in the information infrastructure as possible without compromising on maintainability both by those using the systems internally and externally (e.g. citing in to the information infrastructure, and those responsible for the systems themselves. The migration itself needed to ensure continuity of service and traceability of archived assets.

  12. Metrics of a 'mole hole' against the Lobachevsky space background

    International Nuclear Information System (INIS)

    Tentyukov, M.N.

    1994-01-01

    'Classical' mole hole are the Euclidean metrics consisting of two large space regions connected by a throat. They are the instanton solutions of the Einstein equations. It is shown that for existence of mole holes in the general relativity theory it is required the energy-momentum tensor breaking energetic conditions. 9 refs., 7 figs

  13. Hydatidiform mole in Aminu Kano teaching hospital, northwestern ...

    African Journals Online (AJOL)

    Background: Hydatidiform mole is a benign tumour of the trophoblast tissue and a relatively common gynaecological emergency. It could resolve spontaneously following evacuation, however 9-20% of patients with complete hydatidiform mole go on to have gestational trophoblast neoplasia. It is potentially curable once the ...

  14. Moles: Tool-Assisted Environment Isolation with Closures

    Science.gov (United States)

    de Halleux, Jonathan; Tillmann, Nikolai

    Isolating test cases from environment dependencies is often desirable, as it increases test reliability and reduces test execution time. However, code that calls non-virtual methods or consumes sealed classes is often impossible to test in isolation. Moles is a new lightweight framework which addresses this problem. For any .NET method, Moles allows test-code to provide alternative implementations, given as .NET delegates, for which C# provides very concise syntax while capturing local variables in a closure object. Using code instrumentation, the Moles framework will redirect calls to provided delegates instead of the original methods. The Moles framework is designed to work together with the dynamic symbolic execution tool Pex to enable automated test generation. In a case study, testing code programmed against the Microsoft SharePoint Foundation API, we achieved full code coverage while running tests in isolation without an actual SharePoint server. The Moles framework integrates with .NET and Visual Studio.

  15. Geochemical mole-balance modeling with uncertain data

    Science.gov (United States)

    Parkhurst, David L.

    1997-01-01

    Geochemical mole-balance models are sets of chemical reactions that quantitatively account for changes in the chemical and isotopic composition of water along a flow path. A revised mole-balance formulation that includes an uncertainty term for each chemical and isotopic datum is derived. The revised formulation is comprised of mole-balance equations for each element or element redox state, alkalinity, electrons, solvent water, and each isotope; a charge-balance equation and an equation that relates the uncertainty terms for pH, alkalinity, and total dissolved inorganic carbon for each aqueous solution; inequality constraints on the size of the uncertainty terms; and inequality constraints on the sign of the mole transfer of reactants. The equations and inequality constraints are solved by a modification of the simplex algorithm combined with an exhaustive search for unique combinations of aqueous solutions and reactants for which the equations and inequality constraints can be solved and the uncertainty terms minimized. Additional algorithms find only the simplest mole-balance models and determine the ranges of mixing fractions for each solution and mole transfers for each reactant that are consistent with specified limits on the uncertainty terms. The revised formulation produces simpler and more robust mole-balance models and allows the significance of mixing fractions and mole transfers to be evaluated. In an example from the central Oklahoma aquifer, inclusion of up to 5% uncertainty in the chemical data can reduce the number of reactants in mole-balance models from seven or more to as few as three, these being cation exchange, dolomite dissolution, and silica precipitation. In another example from the Madison aquifer, inclusion of the charge-balance constraint requires significant increases in the mole transfers of calcite, dolomite, and organic matter, which reduce the estimated maximum carbon 14 age of the sample by about 10,000 years, from 22,700 years to

  16. Hydatidiform mole resulting from sexual violence

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    Drezett Jefferson

    2012-02-01

    Full Text Available Abstract Background Hydatidiform mole (HM is characterized by abnormal proliferation of human trophoblast with producers functioning tissues of human chorionic gonadotropin. It can evolve with ovarian cysts tecaluteínicos, hypertension of pregnancy or hyperthyroidism. The incidence of HM is variable and its etiology poorly known, associated with nutritional factors, environmental, age, parity, history of HM, oral contraceptives, smoking, consanguinity or defects in germ cells. There is no reference in literature on HM resulting from sexual violence, objective of this report. Method Description of two cases of HM among 1146 patients with pregnancy resulting from sexual violence treated at Hospital Pérola Byington, São Paulo, from July 1994 to August 2011. Results The cases affected young, white, unmarried, low educated and low parity women. Sexual violence was perpetrated by known offenders unrelated to the victims, under death threat. Ultrasound and CT of the pelvis showed bulky uterus compatible with HM without myometrial invasion. One case was associated with theca lutein cysts. The two cases were diagnosed in the second trimester of pregnancy and evolved with hyperthyroidism. There was no hypertension, disease recurrence, metastasis or sexually transmitted infection. Conclusion The incidence of HM was 1:573 pregnancies resulting from rape, within the range estimated for Latin American countries. Trophoblastic material can be preserved to identify the violence perpetrator, considering only the paternal HM chromosomes. History of sexual violence should be investigated in cases of HM in the first half of adolescence and women in a vulnerable condition.

  17. Microscopic study of edema in hydatidiform mole

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    Olivar C. Castejón

    2014-09-01

    Full Text Available Objectives: the purpose of this study is to use light microscopy and scanning electron microscopy to determine the effect of edema on the structure of the molar vesicle. Methods: samples were taken from the complete hydatidiform mole and processed using conventional light and scanning electron microscopy techniques and an observation protocol that identified four variables: factors underlying the development of edema; the condition of the trophoblast basement membrane, development of the villi, accumulation and degeneration of sulphated mucosubstances at stromal level. Results: light microscopy showed a permeable trophoblastic basement membrane, a swollen syncytium, edematous regions disorganizating the stromal region and causing ischemic necrosis of cells. Using scanning electron microscopy, the basement membrane was found to be distended and thickened, with large irregular holes for the entry and movement of liquid, leaving a wide range of fluids during the influx process and depriving stromal cells of nutrition. Conclusions: a new three-dimensional view of the changes brought about by the entry of fluids into the stroma of molar hydropic vesicles was provided by scanning electron microscopy and confirmed by light microscopy, thereby explaining the changes occurring at the level of the stroma as an effect of the edema.

  18. Genome Stability Maintenance in Naked Mole-Rat.

    Science.gov (United States)

    Petruseva, I O; Evdokimov, A N; Lavrik, O I

    2017-01-01

    The naked mole-rat ( Heterocephalus glaber ) is one of the most promising models used to study genome maintenance systems, including the effective repair of damage to DNA. The naked mole-rat is the longest lived rodent species, which is extraordinarily resistant to cancer and has a number of other unique phenotypic traits. For at least 80% of its lifespan, this animal shows no signs of aging or any increased likelihood of death and retains the ability to reproduce. The naked mole-rat draws the heightened attention of researchers who study the molecular basis of lengthy lifespan and cancer resistance. Despite the fact that the naked mole-rat lives under genotoxic stress conditions (oxidative, etc.), the main characteristics of its genome and proteome are a high stability and effective functioning. Replicative senescence in the somatic cells of naked mole-rats is missing, while an additional p53/pRb-dependent mechanism of early contact inhibition has been revealed in its fibroblasts, which controls cell proliferation and its mechanism of arf- dependent aging. The unique traits of phenotypic and molecular adaptations found in the naked mole-rat speak to a high stability and effective functioning of the molecular machinery that counteract damage accumulation in its genome. This review analyzes existing results in the study of the molecular basis of longevity and high cancer resistance in naked mole-rats.

  19. ACTIVITY PATTERNS IN THE MOLE-RATS TACHYORYCTES ...

    African Journals Online (AJOL)

    Heilophoblus spends over 50 % of the day out of its nest, Tachyoryctes, ..... Surface foraging through an open hole and mole-hill formation could be directly ..... Heliophobius fed at irregular intervals throughout the day and night (Jarvis, ...

  20. Amount of substance and the proposed redefinition of the mole

    Science.gov (United States)

    Milton, M. J. T.; Mills, I. M.

    2009-06-01

    There has been considerable discussion about the merits of redefining four of the base units of the SI, including the mole. In this paper, the options for implementing a new definition for the mole based on a fixed value for the Avogadro constant are discussed. They are placed in the context of the macroscopic nature of the quantity amount of substance and the opportunity to introduce a system for molar and atomic masses with unchanged values and consistent relative uncertainties.

  1. Ao leitor sem medo

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    José Eisenberg

    2000-05-01

    Full Text Available O texto resenha Ao leitor sem medo, de Renato Janine Ribeiro (Belo Horizonte, UFMG, 1999.This text is a review of Ao leitor sem medo by Renato Janine Ribeiro (Belo Horizonte, UFMG, 1999

  2. Diagnosis of hydatidiform mole by hysterography through transabdominal injection

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Chung Kyu; Park, Chang Yun; Choi, Byung Sook [Yonsei University College of Medicine, Seoul (Korea, Republic of)

    1972-03-15

    Molar pregnancy presents a pathologic condition requiring surgery of the uterus promptly as possible although hydatidiform mole itself is a benign disease. In order to remove the mole as quickly as possible, early diagnosis is desirable. In actual practice, however, definite preoperative diagnosis by clinical manifestation is impossible since which is vague or altogether absent symptoms especially in first trimester. Hysterography by transabdominal injection is very valuable and easy procedure for accurate diagnosis of hydatidiform mole which shows typical honey combed, moth eaten or sponge pattern on roentgenography. We have experienced 68 cases of hydatidiform mole during last five and half years. Among them hysterography by transabdominal route was done on 26 cases and shows 96% diagnostic accuracy. One missed case was turned out to be missed abortion. The absence of accidents indicates the harmlessness of the method. We feel the best approach in making diagnosis of hydatidiform mole among clinically borderline and in conclusive for mole pregnancy and its differential diagnosis is by use of transabdominal hysterography.

  3. Diagnosis of hydatidiform mole by hysterography through transabdominal injection

    International Nuclear Information System (INIS)

    Kim, Chung Kyu; Park, Chang Yun; Choi, Byung Sook

    1972-01-01

    Molar pregnancy presents a pathologic condition requiring surgery of the uterus promptly as possible although hydatidiform mole itself is a benign disease. In order to remove the mole as quickly as possible, early diagnosis is desirable. In actual practice, however, definite preoperative diagnosis by clinical manifestation is impossible since which is vague or altogether absent symptoms especially in first trimester. Hysterography by transabdominal injection is very valuable and easy procedure for accurate diagnosis of hydatidiform mole which shows typical honey combed, moth eaten or sponge pattern on roentgenography. We have experienced 68 cases of hydatidiform mole during last five and half years. Among them hysterography by transabdominal route was done on 26 cases and shows 96% diagnostic accuracy. One missed case was turned out to be missed abortion. The absence of accidents indicates the harmlessness of the method. We feel the best approach in making diagnosis of hydatidiform mole among clinically borderline and in conclusive for mole pregnancy and its differential diagnosis is by use of transabdominal hysterography

  4. Mola hidatiforme e doença trofoblástica gestacional Hydatidiform mole and gestational trophoblastic disease

    Directory of Open Access Journals (Sweden)

    Jurandyr Moreira de Andrade

    2009-02-01

    Full Text Available A mola hidatiforme é uma complicação relativamente infrequente da gravidez, mas com potencial para evolução para formas que necessitam de tratamento sistêmico e podem ser ameaçadoras da vida. Sob a denominação de mola hidatiforme há duas entidades histopatológicas e clínicas: a mola parcial e a completa. As diferenças entre estas duas formas são importantes, devido ao risco de evolução para formas persistentes, ou seja, mais alto para as completas. O diagnóstico da mola hidatiforme, seu tratamento e seguimento após o tratamento inicial sofreram alterações importantes nos últimos anos. O número de pacientes assintomáticas tem aumentado devido ao emprego de ultrassonografia no início da gravidez. Para a resolução da mola hidatiforme é necessário evitar o emprego de medicamentos que induzam contrações uterinas e usar a vácuo-aspiração. Deve ser prescrito o método contraceptivo hormonal logo após o esvaziamento da mola. O seguimento é baseado nas dosagens seriadas semanais de gonadotrofinas coriônicas. É importante que o método empregado detecte todas as formas das gonadotrofinas coriônicas (molécula intacta, hiperglicosilada, subunidade β livre e fragmento central da subunidade β.The hydatiform mole is a relatively rare pregnancy complication, but with potential to evolve to forms which need systemic treatment and can be a threat to life. There are two histopathological and clinical entities under the name of hydatiform mole: the partial and the complete mole. The differences between the two forms are important due to risk of evolution to persistent forms, which is higher for the complete moles. The diagnosis, treatment and follow-up of hydatiform mole have been under important changes in the last years. The number of asymptomatic patients has increased, due to the use of ultrasonography at the onset of pregnancy. The use of medication that induces uterine contractions must be avoided, and vacuum

  5. Ultrasonographic findings of Myoma, H-mole and Missed abortion

    International Nuclear Information System (INIS)

    Huh, Nam Yoon; You, H. S.; Seong, K. J.; Park, C. Y.

    1982-01-01

    Ultrasonography is very important in the diagnosis of various kinds of diseases in Obsterics and Gynecology. It has high diagnostic accuracy in the diagnosis of pelvic masses and widely used for the detection of normal orpathologic pregnancy. But still it is difficult to differentiate degenerated myoma, H-mole and missed abortion by ultrasonography. So the authors analyzed the ultrasonographic findings of 81 patients with myoma(29 cases), H-mole(23 cases), and missed abortion(29 cases) and the results are as follows; 1. Diagnostic accuracy was 8.6% in myoma, 87% in H-mole and 89% in missed abortion. 2. The most typical ultrasonographic finding of myoma was obulated mass contour with nonhomogenous internal echo. 3. The most characteristic finding of H-mole was fine vesicular pattern internal echo with globular enlargement of uterus. 4. The most frequent finding of missed abortion was deformed gestational sac with or without remained fetal echo. 5. Clinical correlation was very important for accurate diagnosis, especially when differential diagnosis was very difficult between myoma with marked cystic degeneration, missed abortion with large distorted gestational sac and H-mole with severe degeneration

  6. Extreme hypoxia tolerance of naked mole-rat brain.

    Science.gov (United States)

    Larson, John; Park, Thomas J

    2009-12-09

    Mammalian brains have extremely high levels of aerobic metabolism and typically suffer irreversible damage after brief periods of oxygen deprivation such as occur during stroke or cardiac arrest. Here we report that brain tissue from naked mole-rats, rodents that live in a chronically low-oxygen environment, is remarkably resistant to hypoxia: naked mole-rat neurons maintain synaptic transmission much longer than mouse neurons and can recover from periods of anoxia exceeding 30 min. We suggest that brain tolerance to hypoxia may result from slowed or arrested brain development in these extremely long-lived animals.

  7. Hydatidiform moles among patients with incomplete abortion in ...

    African Journals Online (AJOL)

    SHS

    ated risk factors of HM among patients with incomplete abortion evacuated at Bugando Medical ... Konje E, Massinde A, Rambau P. Hydatidiform moles among patients with ... countries (North America, Australia, New Zealand and ... missed as the cause of abortion. .... and duration of cigarette smoking could not be elicited.

  8. Ground sounds: Seismic detection in the golden mole

    Science.gov (United States)

    Narins, Peter M.; Lewis, Edwin R.

    2004-05-01

    The Namib Desert golden mole is a nocturnal, surface-foraging mammal, possessing a massively hypertrophied malleus which presumably confers low-frequency, substrate-vibration sensitivity through inertial bone conduction. Foraging trails are punctuated with characteristic sand disturbances in which the animal's head dips under the sand. The function of this behavior is not known but it is thought that it may be used to obtain a seismic fix on the next mound to be visited. To test this, we measured the local seismic vibrations both on the top of a mound and on the flats. The spectrum recorded on the flats shows a relatively low-amplitude peak at about 120 Hz, whereas the spectral peak recorded from the mound is nearly 17 dB greater in amplitude and centered at 310 Hz. This suggests that mounds act as seismic beacons for the golden moles that would be detectable from distances corresponding to typical intermound distances of 20-25 m. In addition, out of the 117 species for which data are available, these golden moles have the greatest ossicular mass relative to body size (Mason, personal communication). Functionally, they appear to be low-frequency specialists, and it is likely that golden moles hear through substrate conduction. [Work supported by NIH.

  9. Burrow architecture of the Damaraland mole-rat ( Fukomys ...

    African Journals Online (AJOL)

    The burrow architecture (length, internal dimensions, fractal dimension of tunnel systems, number of nesting chambers and surface mounds) was investigated in the Damaraland mole-rat (Fukomys damarensis). A total of 31 animals were caught from five different colonies and their burrow systems were excavated in their ...

  10. Social status and sex effects on neural morphology in Damaraland mole-rats, Fukomys damarensis.

    Science.gov (United States)

    Anyan, Jeff J; Seney, Marianne L; Holley, Amanda; Bengston, Lynn; Goldman, Bruce D; Forger, Nancy G; Holmes, Melissa M

    2011-01-01

    We previously reported that in a eusocial rodent, the naked mole-rat (Heterocephalus glaber), traditional neural sex differences were absent; instead, neural dimorphisms were associated with breeding status. Here we examined the same neural regions previously studied in naked mole-rats in a second eusocial species, the Damaraland mole-rat (Fukomys damarensis). Damaraland mole-rats live in social groups with breeding restricted to a small number of animals. However, colony sizes are much smaller in Damaraland mole-rats than in naked mole-rats and there is consequently less reproductive skew. In this sense, Damaraland mole-rats may be considered intermediate in social organization between naked mole-rats and more traditional laboratory rodents. We report that, as in naked mole-rats, breeding Damaraland mole-rats have larger volumes of the principal nucleus of the bed nucleus of the stria terminalis and paraventricular nucleus of the hypothalamus than do subordinates, with no effect of sex on these measures. Thus, these structures may play special roles in breeders of eusocial species. However, in contrast to what was seen in naked mole-rats, we also found sex differences in Damaraland mole-rats: volume of the medial amygdala and motoneuron number in Onuf's nucleus were both greater in males than in females, with no significant effect of breeding status. Thus, both sex and breeding status influence neural morphology in Damaraland mole-rats. These findings are in accord with the observed sex differences in body weight and genitalia in Damaraland but not naked mole-rats. We hypothesize that the increased sexual dimorphism in Damaraland mole-rats relative to naked mole-rats is related to reduced reproductive skew. 2011 S. Karger AG, Basel.

  11. For Mole Problems, Call Avogadro: 602-1023

    Science.gov (United States)

    Uthe, R. E.

    2002-10-01

    General education students who have never encountered Avogadro’s number often have difficulty grasping its magnitude and the resulting implications for sizes and numbers of particles in common materials they see around them. I have surveyed the approaches used by several chemical educators. This article describes the techniques I find best help introductory students in General College at the University of Minnesota become familiar with Avogadro’s number and mole calculations. They involve estimating numbers of common objects and then calculating the length of time needed to count large numbers of them. For example, the immense amount of time required to count a mole of sand grains at one grain per second greatly exceeds the age of the universe. The calculations also reinforce procedures for manipulating exponents and applying problem-solving techniques.

  12. A Mole in the Sole: Case Report on Eccrine Poroma

    Directory of Open Access Journals (Sweden)

    Dr. Arnab Ghosh

    2007-01-01

    Full Text Available Eccrine poroma is a benign tumor which arises from the intraepidermal portion of the eccrine sweat glands. 81 year old diabetic grandma worried about her persistently uncontrolled blood sugar owing to a painless mole in the sole of right foot for 2 years. Excisional biopsy revealed a sweat duct benign eccrine poroma. A relationship with diabetes mellitus could not be clearly established in this case.

  13. Severe Hand Injuries Caused by a Mole Gun

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    Serdar Düzgün

    2017-10-01

    Full Text Available Objective: Injuries by mole guns differ from other firearm injuries primarily because they are close-range, low-energy injuries that are highly contaminated owing to contact with contaminated surfaces and the presence of numerous residual foreign bodies within the wound. The aim of this article is to share our surgical experience regarding the repair of severe hand injuries caused by mole guns. Material and Methods: This retrospective study included 11 patients with hand injuries. Data obtained about the patients, including age, gender, dominant or non-dominant hand, injuries to all vital structures, and reconstructive procedures were assessed, categorized, and recorded. Results: Ten patients had defects in one or several common digital branches of the median nerve in areas ranging from the distal part of the carpal tunnel to the distal palmar crease. All patients had flexor and extensor tendon injuries in all fingers except for the first finger. Reconstruction of soft tissue and skin defects was carried out with an interpolation flap planned from a random-based subpectoral- paraumbilical region in five patients, a SCIA-based groin flap in four patients, a reverse-radial forearm flap in one patient, and an adipofascial flap planned from the forearm in one patient. Conclusion: Mole gun injuries typically include all structures of the hand, and repair procedures involve every anatomic structure of the hand. An early and effective surgical operation followed by prolonged and effective physical therapy protocols is vital for regaining the full spectrum of hand functions.

  14. Hydatidiform mole: age-related clinical presentation and high rate of severe complications in older women.

    Science.gov (United States)

    Mangili, Giorgia; Giorgione, Veronica; Gentile, Cinzia; Bergamini, Alice; Pella, Francesca; Almirante, Giada; Candiani, Massimo

    2014-05-01

    The purpose of this study was to demonstrate differences in clinical presentation of hydatidiform mole between women ≥40 years and younger women. Retrospective study. A tertiary referral unit in northern Italy. Three hundred and sixty-five women with hydatidiform mole were divided into group A (Clinical presentation between groups A and B was analyzed, also considering partial hydatidiform mole and complete hydatidiform mole. Differences in clinical presentation according to woman's age. In group B the diagnosis of hydatidiform mole at ≥12 gestational weeks was more frequent (p presented with vaginal bleeding (p clinical features of hydatidiform mole in women ≥40 years are different from those seen in younger women. Failures in the early detection of hydatidiform mole in older women may expose them to a higher rate of severe complications. © 2014 Nordic Federation of Societies of Obstetrics and Gynecology.

  15. Variação anatômica radial do lenho de açoita-cavalo (Luehea divaricata) e sua influência na densidade aparente. Radial variation of wood anatomy in açoita-cavalo (Luehea divaricata) and its influence on the specific gravity.

    OpenAIRE

    Eduardo Luiz LONGUI; Israel Luiz de LIMA; Sandra Monteiro Borges FLORSHEIM; Alaor BUFOLO

    2009-01-01

    Variações entre as dimensões e frequênciasdas células da madeira existem entre espéciesdiferentes, entre indivíduos da mesma espécie ouainda dentro da mesma árvore nas direções radial eaxial. Essa variação influencia diretamente nadensidade aparente e consequentemente no uso dasdiferentes madeiras. O presente estudo determinoua variação anatômica radial e sua influência nadensidade aparente no lenho de árvores de Luehea divaricata, a partir de amostras coletadas no DAP,por meio de método dest...

  16. The risk of persistent trophoblastic disease after hydatidiform mole classified by morphology and ploidy

    DEFF Research Database (Denmark)

    Niemann, Isa; Hansen, Estrid S; Sunde, Lone

    2007-01-01

    classifications, and compared the ability of the two classifications to discriminate between patients with and without a substantial risk of persistent trophoblastic disease. METHODS: 294 cases of consecutively collected hydropic placentas clinically suspected of hydatidiform mole made the basis......OBJECTIVE: Hydatidiform mole can be classified by histopathologic characteristics and by genetic constitutions and most complete moles are diploid, whereas most partial moles are triploid. We investigated the concordance between these two classifications, characterized moles with conflicting......-molar miscarriage, 20 were triploids, 2 were diploid androgenetic and 2 were diploid biparental. In 23% of the conceptuses, the histopathologic and genetic classifications were conflicting. 5% of the patients with hydropic placentas classified as partial mole encountered persistent trophoblastic disease; however...

  17. MOLE 2.0: Advanced approach for analysis of biomacromolecular channels

    OpenAIRE

    Sehnal D.; Varekova R.S.; Berka K.; Pravda L.; Navratilova V.; Banas P.; Ionescu C.-M.; Otyepka M.; Koca J.

    2013-01-01

    Background Channels and pores in biomacromolecules (proteins, nucleic acids and their complexes) play significant biological roles, e.g., in molecular recognition and enzyme substrate specificity. Results We present an advanced software tool entitled MOLE 2.0, which has been designed to analyze molecular channels and pores. Benchmark tests against other available software tools showed that MOLE 2.0 is by comparison quicker, more robust and more versatile. As a new feature, MOLE 2.0 estimates ...

  18. Adult neurogenesis and its anatomical context in the hippocampus of three mole-rat species

    Directory of Open Access Journals (Sweden)

    Irmgard eAmrein

    2014-05-01

    Full Text Available African mole-rats (family Bathyergidae are small to medium sized, long-lived and strictly subterranean rodents that became valuable animal models as a result of their longevity and diversity in social organization. The formation and integration of new hippocampal neurons in adult mammals (adult hippocampal neurogenesis, AHN correlates negatively with age and positively with habitat complexity. Here we present quantitative data on AHN in wild-derived mole-rats of one year and older, and briefly describe its anatomical context including markers of neuronal function (calbindin and parvalbumin. Solitary Cape mole-rats (Georychus capensis, social highveld mole-rats (Cryptomys hottentotus pretoriae, and eusocial naked mole-rats (Heterocephalus glaber were assessed. Compared to other rodents, the hippocampal formation in mole-rats is small, but shows a distinct cytoarchitecture in the dentate gyrus and CA1. Distributions of the calcium-binding proteins differ from those seen in rodents; e.g., calbindin in CA3 of naked mole-rats distributes similar to the pattern seen in early primate development, and calbindin staining extends into the stratum lacunosum-moleculare of Cape mole-rats. Proliferating cells and young neurons are found in low numbers in the hippocampus of all three mole-rat species. Resident granule cell numbers are low as well. Proliferating cells expressed as a percentage of resident granule cells are in the range of other rodents, while the percentage of young neurons is lower than that observed in surface dwelling rodents. Between mole-rat species, we observed no difference in the percentage of proliferating cells. The percentages of young neurons are high in social highveld and naked mole-rats, and low in solitary Cape mole-rats. The findings support that proliferation is regulated independently of average life expectancy and habitat. Instead, neuronal differentiation reflects species-specific demands, which appear lower in subterranean

  19. Adult neurogenesis and its anatomical context in the hippocampus of three mole-rat species.

    Science.gov (United States)

    Amrein, Irmgard; Becker, Anton S; Engler, Stefanie; Huang, Shih-Hui; Müller, Julian; Slomianka, Lutz; Oosthuizen, Maria K

    2014-01-01

    African mole-rats (family Bathyergidae) are small to medium sized, long-lived, and strictly subterranean rodents that became valuable animal models as a result of their longevity and diversity in social organization. The formation and integration of new hippocampal neurons in adult mammals (adult hippocampal neurogenesis, AHN) correlates negatively with age and positively with habitat complexity. Here we present quantitative data on AHN in wild-derived mole-rats of 1 year and older, and briefly describe its anatomical context including markers of neuronal function (calbindin and parvalbumin). Solitary Cape mole-rats (Georychus capensis), social highveld mole-rats (Cryptomys hottentotus pretoriae), and eusocial naked mole-rats (Heterocephalus glaber) were assessed. Compared to other rodents, the hippocampal formation in mole-rats is small, but shows a distinct cytoarchitecture in the dentate gyrus and CA1. Distributions of the calcium-binding proteins differ from those seen in rodents; e.g., calbindin in CA3 of naked mole-rats distributes similar to the pattern seen in early primate development, and calbindin staining extends into the stratum lacunosum-moleculare of Cape mole-rats. Proliferating cells and young neurons are found in low numbers in the hippocampus of all three mole-rat species. Resident granule cell numbers are low as well. Proliferating cells expressed as a percentage of resident granule cells are in the range of other rodents, while the percentage of young neurons is lower than that observed in surface dwelling rodents. Between mole-rat species, we observed no difference in the percentage of proliferating cells. The percentages of young neurons are high in social highveld and naked mole-rats, and low in solitary Cape mole-rats. The findings support that proliferation is regulated independently of average life expectancy and habitat. Instead, neuronal differentiation reflects species-specific demands, which appear lower in subterranean rodents.

  20. High molecular weight hyaluronan mediates the cancer resistance of the naked mole-rat

    Science.gov (United States)

    Tian, Xiao; Azpurua, Jorge; Hine, Christopher; Vaidya, Amita; Myakishev-Rempel, Max; Ablaeva, Julia; Mao, Zhiyong; Nevo, Eviatar; Gorbunova, Vera; Seluanov, Andrei

    2013-01-01

    The naked mole-rat displays exceptional longevity, with a maximum lifespan exceeding 30 years1–3. This is the longest reported lifespan for a rodent species and is especially striking considering the small body mass of the naked mole-rat. In comparison, a similarly sized house mouse has a maximum lifespan of 4 years4,5. In addition to their longevity, naked mole-rats show an unusual resistance to cancer. Multi-year observations of large naked mole-rat colonies did not detect a single incidence of cancer2,6. Here we identify a mechanism responsible for the naked mole-rat’s cancer resistance. We found that naked mole-rat fibroblasts secrete extremely high molecular weight hyaluronan (HA), which is over five times larger than human or mouse HA. This high molecular weight HA accumulates abundantly in naked mole rat tissues due to the decreased activity of HA-degrading enzymes and a unique sequence of hyaluronan synthase 2 (HAS2). Furthermore, the naked mole-rat cells are more sensitive to HA signaling, as the naked mole rat cells have a higher affinity to HA than the mouse or human cells. Perturbation of the signaling pathways sufficient for malignant transformation of mouse fibroblasts fails to transform naked mole-rat cells. However, once high molecular weight HA is removed by either knocking down HAS2 or overexpressing the HA-degrading enzyme, Hyal2, naked mole-rat cells become susceptible to malignant transformation and readily form tumors in mice. We speculate that naked mole-rats have evolved a higher concentration of HA in the skin to provide skin elasticity needed for life in underground tunnels. This trait may have then been co-opted to provide cancer resistance and longevity to this species. PMID:23783513

  1. [Complete hydatiforme mole in Morocco: epidemiological and clinical study].

    Science.gov (United States)

    Boufettal, H; Coullin, P; Mahdaoui, S; Noun, M; Hermas, S; Samouh, N

    2011-09-01

    Complete hydatidiform moles (CHM) are a real public health problem, especially in the "southern countries" and Asia, because of their impact on the female reproduction and the risk to progression to either invasive mole or choriocarcinoma. We collected the cases of CHM referred to our department over a period of ten years (2000 to 2009). We will present our results, emphasize the modalities of diagnosis, treatment and evolution, with a review of literature. During this study, we identified 254 cases of CHM, and recorded 57,987 births and 1627 abortions. Their incidence was 0.43% of pregnancies. The mean age of our patients is 25 years old (16 to 55). Relative risk observed was much increased among women under 20 years old (×6.8) and those over 40 years old (×15). Both of nulliparous and primiparous patients represented 52.3% of the cohort. Eighty-five percent of patients belonged to an agricultural environment associated with a low socio-economic status. Uterine bleeding was the most common symptom accounting for 93.7%. Toxic syndrome was present in 18.5% of patients. Physical examination showed a highly increased uterine size in 85% of cases associated with lateral uterine mass in 25% of cases. The diagnosis was suspected using ultrasonography in all cases associated with an elevated level of plasmatic β-human chorionic gonadotrophin (βhCG). All cases were confirmed histologically. Treatment used was endo-uterine aspiration in all cases. Recurrence of CHM was documented in 25 patients or 9.4%. Neoplasic progression was observed for 6.3% of cases. All of them have evolved into remission with chemotherapy. CHM continue to be a public health problem in Morocco, their incidence is among the highest ones. In fact, this studied population corresponds to the lowest socio-economic status and generally described as population at risk. It is subject to drastic weather's conditions causing loss of fresh products. Extreme ages and degree of parity are also risk factors

  2. Electrical Activation Studies of Silicon Implanted Aluminum Gallium Nitride with High Aluminum Mole Fraction

    National Research Council Canada - National Science Library

    Moore, Elizabeth A

    2007-01-01

    ...) alloys, and represents a comprehensive analysis of the resulting material's electrical and optical properties as a function of Al mole fraction, anneal temperature, anneal time, and implantation dose...

  3. High molecular weight hyaluronan mediates the cancer resistance of the naked mole-rat

    OpenAIRE

    Tian, Xiao; Azpurua, Jorge; Hine, Christopher; Vaidya, Amita; Myakishev-Rempel, Max; Ablaeva, Julia; Mao, Zhiyong; Nevo, Eviatar; Gorbunova, Vera; Seluanov, Andrei

    2013-01-01

    The naked mole-rat displays exceptional longevity, with a maximum lifespan exceeding 30 years 1–3 . This is the longest reported lifespan for a rodent species and is especially striking considering the small body mass of the naked mole-rat. In comparison, a similarly sized house mouse has a maximum lifespan of 4 years 4,5 . In addition to their longevity, naked mole-rats show an unusual resistance to cancer. Multi-year observations of large naked mole-rat colonies did not detect a single inci...

  4. Application of the MOLE in post-nuclear accident characterization

    International Nuclear Information System (INIS)

    Johnson, S.J.; Alvarez, J.L.

    1981-01-01

    Following a nuclear accident there is a need to determine the chemical composition of materials in liquid, solid and gaseous form, the crystalline structure of solids, the size and chemical composition of particles, and the chemical characterization of contaminants on surfaces. This analytical information is required to reconstruct the accident scenario, to select decontamination methods, and to determine future safety requirements. The MOLE (Molecular Optical Laser Examiner) is a Raman microprobe system which has proven to be a valuable analytical tool in providing this type of chemical information. It can determine the chemical species of polyatomic molecules and ions having characteristic Raman spectra. As little as 1 picogram of a component or a 1 μm particle can be analyzed. The imaging system can also provide mapping of selected components on a surface. A system description, sample handling techniques, and applications are presented. Specific applications to the Three Mile Island-Unit 2 accident are also addressed

  5. Microwave Determination of Water Mole Fraction in Humid Gas Mixtures

    Science.gov (United States)

    Cuccaro, R.; Gavioso, R. M.; Benedetto, G.; Madonna Ripa, D.; Fernicola, V.; Guianvarc'h, C.

    2012-09-01

    A small volume (65 cm3) gold-plated quasi-spherical microwave resonator has been used to measure the water vapor mole fraction x w of H2O/N2 and H2O/air mixtures. This experimental technique exploits the high precision achievable in the determination of the cavity microwave resonance frequencies and is particularly sensitive to the presence of small concentrations of water vapor as a result of the high polarizability of this substance. The mixtures were prepared using the INRIM standard humidity generator for frost-point temperatures T fp in the range between 241 K and 270 K and a commercial two-pressure humidity generator operated at a dew-point temperature between 272 K and 291 K. The experimental measurements compare favorably with the calculated molar fractions of the mixture supplied by the humidity generators, showing a normalized error lower than 0.8.

  6. Placentation in the Hottentot golden mole, Amblysomus hottentotus (Afrosoricida: Chrysochloridae)

    DEFF Research Database (Denmark)

    Jones, C J P; Carter, A M; Bennett, N C

    2009-01-01

    The placentation of the Hottentot golden mole (Amblysomus hottentotus) has been examined using light and electron microscopy and lectin histochemistry of nine specimens at both mid and late gestation. The placentae were lobulated towards the allantoic surface and the lobules contained roughly...... parallel arrays of labyrinthine structures converging on a central spongy zone. At mid gestation, the arrays were composed of an inner cellular and outer syncytial trophoblast layer, the inner layer enclosing scant connective tissue and fetal capillaries. Maternal blood spaces coursed through the outer...... trophoblast and were lined by trophoblastic microvilli; the blood spaces were narrow in mid gestation but enlarged near term, while the inner trophoblast layer became thinner and seemed to be syncytial. These features were confirmed by transmission electron microscopy. The microvillous surfaces and dispersed...

  7. Variação do incremento anual ao longo do fuste de Pinus taeda L. em diferentes idades e densidades populacionais.

    Directory of Open Access Journals (Sweden)

    César Martins Andrade

    2010-08-01

    Full Text Available A variação do crescimento anual em diferentes alturas do fuste de Pinus taeda L. foi estudada por meio da análise de tronco em árvores do estrato dominante e médio. Foram coletadas secções transversais ao longo do fuste de 9 árvores-amostra, tomadas na base, à altura do peito (DAP, e após, a cada metro. A amostragem foi constituída de 4 árvores provenientes de Canela, Rio Grande do Sul, em floresta com 17 anos de idade, submetida a um desbaste, e 5 árvores de Ponte Alta do Norte, Santa Catarina, em floresta com 34 anos, submetida a cinco desbastes. Os resultados, expressos na forma percentual do incremento em área basal na posição "i", sobre o incremento em área basal ao nível do DAP, mostraram a forte variação na deposição anual de lenho ao longo do fuste em função da densidade populacional, caracterizando os efeitos da competição e da liberação pela execução do desbaste. Árvores sujeitas à competição mostraram maior crescimento nas partes superiores do fuste, enquanto que árvores sob menor concorrência apresentaram maior crescimento em posições inferiores, facilmente identificadas pela forma e inclinação das curvas de incremento.

  8. A phylogenetic estimate for golden moles (Mammalia, Afrotheria, Chrysochloridae

    Directory of Open Access Journals (Sweden)

    Bloomer Paulette

    2010-03-01

    Full Text Available Abstract Background Golden moles (Chrysochloridae are small, subterranean, afrotherian mammals from South Africa and neighboring regions. Of the 21 species now recognized, some (e.g., Chrysochloris asiatica, Amblysomus hottentotus are relatively common, whereas others (e.g., species of Chrysospalax, Cryptochloris, Neamblysomus are rare and endangered. Here, we use a combined analysis of partial sequences of the nuclear GHR gene and morphological characters to derive a phylogeny of species in the family Chrysochloridae. Results Although not all nodes of the combined analysis have high support values, the overall pattern of relationships obtained from different methods of phylogeny reconstruction allow us to make several recommendations regarding the current taxonomy of golden moles. We elevate Huetia to generic status to include the species leucorhinus and confirm the use of the Linnean binomial Carpitalpa arendsi, which belongs within Amblysominae along with Amblysomus and Neamblysomus. A second group, Chrysochlorinae, includes Chrysochloris, Cryptochloris, Huetia, Eremitalpa, Chrysospalax, and Calcochloris. Bayesian methods make chrysochlorines paraphyletic by placing the root within them, coinciding with root positions favored by a majority of randomly-generated outgroup taxa. Maximum Parsimony (MP places the root either between chrysochlorines and amblysomines (with Chlorotalpa as sister taxon to amblysomines, or at Chlorotalpa, with the former two groups reconstructed as monophyletic in all optimal MP trees. Conclusions The inclusion of additional genetic loci for this clade is important to confirm our taxonomic results and resolve the chrysochlorid root. Nevertheless, our optimal topologies support a division of chrysochlorids into amblysomines and chrysochlorines, with Chlorotalpa intermediate between the two. Furthermore, evolution of the chrysochlorid malleus exhibits homoplasy. The elongate malleus has evolved just once in the

  9. A phylogenetic estimate for golden moles (Mammalia, Afrotheria, Chrysochloridae).

    Science.gov (United States)

    Asher, Robert J; Maree, Sarita; Bronner, Gary; Bennett, Nigel C; Bloomer, Paulette; Czechowski, Paul; Meyer, Matthias; Hofreiter, Michael

    2010-03-09

    Golden moles (Chrysochloridae) are small, subterranean, afrotherian mammals from South Africa and neighboring regions. Of the 21 species now recognized, some (e.g., Chrysochloris asiatica, Amblysomus hottentotus) are relatively common, whereas others (e.g., species of Chrysospalax, Cryptochloris, Neamblysomus) are rare and endangered. Here, we use a combined analysis of partial sequences of the nuclear GHR gene and morphological characters to derive a phylogeny of species in the family Chrysochloridae. Although not all nodes of the combined analysis have high support values, the overall pattern of relationships obtained from different methods of phylogeny reconstruction allow us to make several recommendations regarding the current taxonomy of golden moles. We elevate Huetia to generic status to include the species leucorhinus and confirm the use of the Linnean binomial Carpitalpa arendsi, which belongs within Amblysominae along with Amblysomus and Neamblysomus. A second group, Chrysochlorinae, includes Chrysochloris, Cryptochloris, Huetia, Eremitalpa, Chrysospalax, and Calcochloris. Bayesian methods make chrysochlorines paraphyletic by placing the root within them, coinciding with root positions favored by a majority of randomly-generated outgroup taxa. Maximum Parsimony (MP) places the root either between chrysochlorines and amblysomines (with Chlorotalpa as sister taxon to amblysomines), or at Chlorotalpa, with the former two groups reconstructed as monophyletic in all optimal MP trees. The inclusion of additional genetic loci for this clade is important to confirm our taxonomic results and resolve the chrysochlorid root. Nevertheless, our optimal topologies support a division of chrysochlorids into amblysomines and chrysochlorines, with Chlorotalpa intermediate between the two. Furthermore, evolution of the chrysochlorid malleus exhibits homoplasy. The elongate malleus has evolved just once in the Cryptochloris-Chrysochloris group; other changes in shape have

  10. Comparative morphology of the penis and clitoris in four species of moles (Talpidae)

    Science.gov (United States)

    Sinclair, Adriane Watkins; Glickman, Stephen; Catania, Kenneth; Shinohara, Akio; Baskin, Lawrence; Cunha, Gerald R.

    2017-01-01

    The penile and clitorial anatomy of four species of Talpid moles (broad-footed, star-nosed, hairy-tailed, and Japanese shrew moles) were investigated to define penile and clitoral anatomy and to examine the relationship of the clitoral anatomy with the presence or absence of ovotestes. The ovotestis contains ovarian tissue and glandular tissue resembling fetal testicular tissue and can produce androgens. The ovotestis is present in star-nosed and hairy-tailed moles, but not in broad-footed and Japanese shrew moles. Using histology, 3D reconstruction, and morphometric analysis, sexual dimorphism was examined in regard to a nine feature masculine trait score that included perineal appendage length (prepuce), anogenital distance, and presence/absence of bone. The presence/absence of ovotestes was discordant in all four mole species for sex differentiation features. For many sex differentiation features, discordance with ovotestes was observed in at least one mole species. The degree of concordance with ovotestes was highest for hairy-tailed moles and lowest for broad-footed moles. In relationship to phylogenetic clade, sex differentiation features also did not correlate with the similarity/divergence of the features and presence/absence of ovotestes. Hairy-tailed and Japanese shrew moles reside in separated clades, but they exhibit a high degree of congruence. Broad-footed and hairy-tailed moles reside within the same clade but had one of the lowest correlations in features and presence/absence of ovotestes. Thus, phylogenetic affinity and the presence/absence of ovotestes are poor predictors for most sex differentiation features within mole external genitalia. PMID:28251823

  11. High-molecular-mass hyaluronan mediates the cancer resistance of the naked mole rat.

    Science.gov (United States)

    Tian, Xiao; Azpurua, Jorge; Hine, Christopher; Vaidya, Amita; Myakishev-Rempel, Max; Ablaeva, Julia; Mao, Zhiyong; Nevo, Eviatar; Gorbunova, Vera; Seluanov, Andrei

    2013-07-18

    The naked mole rat (Heterocephalus glaber) displays exceptional longevity, with a maximum lifespan exceeding 30 years. This is the longest reported lifespan for a rodent species and is especially striking considering the small body mass of the naked mole rat. In comparison, a similarly sized house mouse has a maximum lifespan of 4 years. In addition to their longevity, naked mole rats show an unusual resistance to cancer. Multi-year observations of large naked mole-rat colonies did not detect a single incidence of cancer. Here we identify a mechanism responsible for the naked mole rat's cancer resistance. We found that naked mole-rat fibroblasts secrete extremely high-molecular-mass hyaluronan (HA), which is over five times larger than human or mouse HA. This high-molecular-mass HA accumulates abundantly in naked mole-rat tissues owing to the decreased activity of HA-degrading enzymes and a unique sequence of hyaluronan synthase 2 (HAS2). Furthermore, the naked mole-rat cells are more sensitive to HA signalling, as they have a higher affinity to HA compared with mouse or human cells. Perturbation of the signalling pathways sufficient for malignant transformation of mouse fibroblasts fails to transform naked mole-rat cells. However, once high-molecular-mass HA is removed by either knocking down HAS2 or overexpressing the HA-degrading enzyme, HYAL2, naked mole-rat cells become susceptible to malignant transformation and readily form tumours in mice. We speculate that naked mole rats have evolved a higher concentration of HA in the skin to provide skin elasticity needed for life in underground tunnels. This trait may have then been co-opted to provide cancer resistance and longevity to this species.

  12. Trading new neurons for status: Adult hippocampal neurogenesis in eusocial Damaraland mole-rats.

    Science.gov (United States)

    Oosthuizen, M K; Amrein, I

    2016-06-02

    Diversity in social structures, from solitary to eusocial, is a prominent feature of subterranean African mole-rat species. Damaraland mole-rats are eusocial, they live in colonies that are characterized by a reproductive division of labor and a subdivision into castes based on physiology and behavior. Damaraland mole-rats are exceptionally long lived and reproductive animals show delayed aging compared to non-reproductive animals. In the present study, we described the hippocampal architecture and the rate of hippocampal neurogenesis of wild-derived, adult Damaraland mole-rats in relation to sex, relative age and social status or caste. Overall, Damaraland mole-rats were found to have a small hippocampus and low rates of neurogenesis. We found no correlation between neurogenesis and sex or relative age. Social status or caste was the most prominent modulator of neurogenesis. An inverse relationship between neurogenesis and social status was apparent, with queens displaying the lowest neurogenesis while the worker mole-rats had the most. As there is no natural progression from one caste to another, social status within a colony was relatively stable and is reflected in the level of neurogenesis. Our results correspond to those found in the naked mole-rat, and may reflect an evolutionary and environmentally conserved trait within social mole-rat species. Copyright © 2016 IBRO. Published by Elsevier Ltd. All rights reserved.

  13. MOLED: Simulation of multilayer organic light emitting diodes

    Science.gov (United States)

    Houili, H.; Tutiš, E.; Lütjens, H.; Bussac, M. N.; Zuppiroli, L.

    2003-12-01

    MOLED solves the dynamics of electrons and holes in multilayer Organic Light Emitting Diodes (OLED). The carriers are injected on the positive and negative electrodes of the device by tunneling through a potential barrier. Thermal excitation processes across the barrier are also included. In the interior of the device the electron-hole recombination occurs when the two carriers are close enough, according to a model inspired from the one of Langevin. A fraction of these recombined pairs gives photons. The charge transport inside the organic material occurs through hopping. Several choices of mobility formulae are available in the code. MOLED can be used for OLEDs with an arbitrary number of layers. The output consists of numerous fields that describe the device performance. For example, there are the current, the recombination and the charge density distributions, the electric field distribution, the current-voltage characteristics and the device internal quantum efficiency. Program summaryTitle of program: MOLED Catalogue identifier: ADSG Program summary URL:http://cpc.cs.qub.ac.uk/summaries/ADSG Program obtainable from: CPC Program Library, Queen's University of Belfast, N. Ireland Operating systems under which the program has been tested: Unix, Linux Programming language used: FORTRAN 90 Memory required to execute with typical data: 2 MB No. of bytes in distributed program: 26 942 No. of bits in a word: 64 Peripherals used: permanent disk storage No. of lines in distributed program, including test data, etc.: 3695 Distribution format: tar gzip file Nature of the physical problem: Injection of electrons and holes into an organic electroluminescent material occurs through tunneling from metal electrodes. The transport of carriers inside the molecular medium proceeds by hopping from one molecule to another. The emission of light is a result of their radiative Langevin recombination (for a review see [Scott et al., Synthetic Metals 111-112 (2000) 289; Friend et al

  14. Genome sequencing reveals insights into physiology and longevity of the naked mole rat

    DEFF Research Database (Denmark)

    Kim, Eun Bae; Fang, Xiaodong; Fushan, Alexey A

    2011-01-01

    The naked mole rat (Heterocephalus glaber) is a strictly subterranean, extraordinarily long-lived eusocial mammal. Although it is the size of a mouse, its maximum lifespan exceeds 30 years, making this animal the longest-living rodent. Naked mole rats show negligible senescence, no age......-related increase in mortality, and high fecundity until death. In addition to delayed ageing, they are resistant to both spontaneous cancer and experimentally induced tumorigenesis. Naked mole rats pose a challenge to the theories that link ageing, cancer and redox homeostasis. Although characterized...... by significant oxidative stress, the naked mole rat proteome does not show age-related susceptibility to oxidative damage or increased ubiquitination. Naked mole rats naturally reside in large colonies with a single breeding female, the 'queen', who suppresses the sexual maturity of her subordinates. They also...

  15. The Naked Mole Rat Genome Resource: facilitating analyses of cancer and longevity-related adaptations.

    Science.gov (United States)

    Keane, Michael; Craig, Thomas; Alföldi, Jessica; Berlin, Aaron M; Johnson, Jeremy; Seluanov, Andrei; Gorbunova, Vera; Di Palma, Federica; Lindblad-Toh, Kerstin; Church, George M; de Magalhães, João Pedro

    2014-12-15

    The naked mole rat (Heterocephalus glaber) is an exceptionally long-lived and cancer-resistant rodent native to East Africa. Although its genome was previously sequenced, here we report a new assembly sequenced by us with substantially higher N50 values for scaffolds and contigs. We analyzed the annotation of this new improved assembly and identified candidate genomic adaptations which may have contributed to the evolution of the naked mole rat's extraordinary traits, including in regions of p53, and the hyaluronan receptors CD44 and HMMR (RHAMM). Furthermore, we developed a freely available web portal, the Naked Mole Rat Genome Resource (http://www.naked-mole-rat.org), featuring the data and results of our analysis, to assist researchers interested in the genome and genes of the naked mole rat, and also to facilitate further studies on this fascinating species. © The Author 2014. Published by Oxford University Press.

  16. Acanthocephala Parasite (Profilicollis spp.) Loads in Correlation to Pacific Mole Crab (Emerita analoga) Size

    Science.gov (United States)

    Cheng, T.; Huang, S.; Galathe, M.; Jenkins, M.; Ramirez, A.; Crosby, L.; Barrera, J.; FitzHoward, S.

    2013-12-01

    Since 2002, San Francisco Bay students have been conducting marine ecosystem monitoring through a joint project with the Long-term Monitoring Program and Experiential Training for Students (LiMPETS), in conjunction with the Gulf of Farallones National Marine Sanctuary. Each year students collect population and demographic data on Pacific mole crabs (Emerita analoga), an indicator species that lives in the sandy beach habitat in temperate regions along the Pacific Ocean. Pacific mole crabs are filter feeding crustaceans that inhabit the intertidal swash zone and are known to be an intermediate host for parasitic ';spiny-headed' worms in the phylum Acanthocephala (Profilicollis spp.). Sampling takes place during their reproductive period, which occurs from spring to fall, and includes measuring total body length of the Pacific mole crabs and dissecting them to determine presence of Acanthocephalan parasites. We hypothesize that due to larger body mass, larger Pacific mole crabs will have a greater number of Acanthocephala parasites.We conducted several analyses using the LiMPETS long-term data. Specifically, we compared body length, crab gender, and parasite abundance from Pacific mole crabs sampled from four beaches located in the county and city of San Francisco. Our results indicated that larger Pacific mole crabs do not necessarily have more parasites, but are more likely to have at least one parasite, while female Pacific mole crabs carrying eggs, have more parasites than males or females without eggs. We also found that parasite loads per mole crab was highest in the spring. Further analysis will be conducted to determine factors affecting Pacific mole crab parasite loads. Studying Pacific mole crabs help evaluate the health of California's intertidal systems and how human activities, geologic changes, and climate changes all make huge impacts to the intertidal ecosystems.

  17. Tecidos moles (não resistentes: como se fossilizam?

    Directory of Open Access Journals (Sweden)

    Silvia Regina Gobbo

    2015-06-01

    Full Text Available A preservação de tecidos moles (não resistentes desperta atenção pela qualidade dos fósseis preservados, e pela raridade. Este trabalho faz ampla revisão do assunto, desfaz alguns dos mitos correntes sobre fossilização destes materiais, como anaerobiose e soterramento rápido, bem como assinala a relevância do papel da replicagem de estruturas morfológicas por colônias microbianas. No âmbito dos fatores que influenciam a preservação de tecidos não resistentes estão pH, processos de decomposição, soterramento rápido e mineralização rápida por meio de carbonatos ou fosfatos. É possível citar exemplos de fácies sedimentares nas quais há preservação de tecidos não resistentes, como os folhelhos pirobetuminosos, calcários litográficos, folhelhos e diatomitos. Destaca-se o papel das colônias microbianas, tanto na forma de microesferas, como aquelas não-esferulíticas. Os três mitos principais que se procurou desfazer no artigo são: (1 a presença de oxigênio; (2 o soterramento rápido e (3 as colônias microbianas.

  18. Carta ao Leitor

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    Editorial Editorial

    2014-02-01

    Full Text Available ApresentaçãoO Centro de Educação a Distância – CEAD/Unimontes foi inaugurado em 2011 em histórico de ricas experiências vivenciadas pela Universidade Estadual de Montes Claros – Unimontes ao longo de 15 anos.Uma jornada de esforços conjuntos e uma estima apaixonada pela educação e pelo conheci-mento científico carecia, em seu núcleo, de uma plataforma que reunisse todas as perspectivas constitutivas de sua história e finalidade. Um anseio crescente da instituição que se desdobra e desdobrará em produtos e iniciativas de fomento em pesquisa. Em 2012, como fruto gerado di-retamente do âmago dessas necessidades unidas ao cenário nacional de valorização crescente da pesquisa científica, surge a Revista Multitexto.A Revista Multitexto nasce como um periódico em movimento. São contribuições de aborda-gens múltiplas num ensejo genuíno e único de progressão científica em pesquisa. Um marco para os estudos na área de ensino a distância numa oferta interdisciplinar. Unidade na diversidade: a grande proposta da revista. Espaço aberto para pesquisadores e entusiastas. Multipliquem-se os multitextos em contribuições múltiplas.Sobre a Revista MultitextoPublicação semestral do Centro de Educação a Distância da Universidade Estadual de Montes Claros – CEAD/Unimontes, que contribui na área de conhecimento produzida pelas fenomenologias e áreas correlatas em Educação a Distância, com foco interdisciplinar. Aberta em 2012, a Revista Multitexto recebe colaborações de pesquisadores em ensino a dis-tância e demais áreas. É meta referencial da revista abordar questões conceituais e metodológicas, bem como, os desenvolvimentos da pesquisa, aplicada ou teórica, na área de educação à distância (EAD; difundir a produção científica de pesquisadores da área de educação à distância (EAD, inseridos em Institutos de pesquisa e de educação superior no Brasil; propiciar um espaço para o

  19. agradecimento aos consultores ad hoc

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    REA Editor

    2012-02-01

    Full Text Available Agradecemos aos professores Nildo Viana (UFG e Flavio Sofiati (UFG, organizadores do DOSSIÊ JUVENTUDE & SOCIEDADE, e aos Consultores Ad hoc pela leitura e apreciação crítica dos artigos submetidos e publicados nesta edição.

  20. Partial hydatidiform mole with false-negative urine human chorionic gonadatropin test in the emergency department.

    Science.gov (United States)

    Mundangepfupfu, Tichaendepi; Waseem, Muhammad

    2014-03-01

    Hydatidiform mole (molar pregnancy) is a benign tumor of placental trophoblastic cells, which release human chorionic gonadotropin (hCG). Several case reports have described complete hydatidiform moles with false-negative urine qualitative hCG tests. These negative pregnancy tests have been attributed to the hook effect. We report an unusual presentation of a partial mole and review an alternative explanation for the negative hCG test. As partial moles are usually not associated with a large proliferation of trophoblastic cells, levels of hCG are commonly negative and serum quantitative hCG was 1,094,950 mIU/mL. Pelvic ultrasonography showed a uterine cavity containing a soft-tissue mass with multiple cystic lesions and the hydatidiform mole was extracted with suction curettage. Tissue pathology confirmed partial hydatidiform mole. In addition to the hook effect, we present another possible explanation for the false-negative test; namely the inability of some assays to detect hCG-degradation products, which may be higher in clinical samples from patients with hydatidiform mole. This case underscores the importance of knowing the limitations of the commonly used hCG assays. Copyright © 2014 Elsevier Inc. All rights reserved.

  1. Sleep in the Cape Mole Rat: A Short-Sleeping Subterranean Rodent.

    Science.gov (United States)

    Kruger, Jean-Leigh; Gravett, Nadine; Bhagwandin, Adhil; Bennett, Nigel C; Archer, Elizabeth K; Manger, Paul R

    2016-01-01

    The Cape mole rat Georychus capensis is a solitary subterranean rodent found in the western and southern Cape of South Africa. This approximately 200-gram bathyergid rodent shows a nocturnal circadian rhythm, but sleep in this species is yet to be investigated. Using telemetric recordings of the electroencephalogram (EEG) and electromyogram (EMG) in conjunction with video recordings, we were able to show that the Cape mole rat, like all other rodents, has sleep periods composed of both rapid eye movement (REM) and slow-wave (non-REM) sleep. These mole rats spent on average 15.4 h awake, 7.1 h in non-REM sleep and 1.5 h in REM sleep each day. Cape mole rats sleep substantially less than other similarly sized terrestrial rodents but have a similar percentage of total sleep time occupied by REM sleep. In addition, the duration of both non-REM and REM sleep episodes was markedly shorter in the Cape mole rat than has been observed in terrestrial rodents. Interestingly, these features (total sleep time and episode duration) are similar to those observed in another subterranean bathyergid mole rat, i.e. Fukomys mechowii. Thus, there appears to be a bathyergid type of sleep amongst the rodents that may be related to their environment and the effect of this on their circadian rhythm. Investigating further species of bathyergid mole rats may fully define the emerging picture of sleep in these subterranean African rodents. © 2016 S. Karger AG, Basel.

  2. Anatomia ecológica e crescimento do lenho de Sebastiania commersoniana (Baillon) Smith & Downs, em diferentes condiçoes geomorfológicas e pedológicas da planície do Rio Iguaçu - PR

    OpenAIRE

    Cosmo, Nelson Luiz

    2013-01-01

    Pretendeu-se neste trabalho analisar a anatomia do lenho e o crescimento de Sebastiania commersoniana (branquilho), visando identificar possíveis respostas desta espécie às condições ecológicas das planícies fluviais. Nestes ambientes, a espécie tem ampla distribuição, graças à sua capacidade de se estabelecer em diversas condições geomorfológicas e pedológicas. Foram avaliadas árvores crescendo em três áreas de duas localidades distintas na planície do rio Iguaçu. Em Guajuvira, município da ...

  3. Selective inflammatory pain insensitivity in the African naked mole-rat (Heterocephalus glaber).

    Science.gov (United States)

    Park, Thomas J; Lu, Ying; Jüttner, René; Smith, Ewan St J; Hu, Jing; Brand, Antje; Wetzel, Christiane; Milenkovic, Nevena; Erdmann, Bettina; Heppenstall, Paul A; Laurito, Charles E; Wilson, Steven P; Lewin, Gary R

    2008-01-01

    In all mammals, tissue inflammation leads to pain and behavioral sensitization to thermal and mechanical stimuli called hyperalgesia. We studied pain mechanisms in the African naked mole-rat, an unusual rodent species that lacks pain-related neuropeptides (e.g., substance P) in cutaneous sensory fibers. Naked mole-rats show a unique and remarkable lack of pain-related behaviors to two potent algogens, acid and capsaicin. Furthermore, when exposed to inflammatory insults or known mediators, naked mole-rats do not display thermal hyperalgesia. In contrast, naked mole-rats do display nocifensive behaviors in the formalin test and show mechanical hyperalgesia after inflammation. Using electrophysiology, we showed that primary afferent nociceptors in naked mole-rats are insensitive to acid stimuli, consistent with the animal's lack of acid-induced behavior. Acid transduction by sensory neurons is observed in birds, amphibians, and fish, which suggests that this tranduction mechanism has been selectively disabled in the naked mole-rat in the course of its evolution. In contrast, nociceptors do respond vigorously to capsaicin, and we also show that sensory neurons express a transient receptor potential vanilloid channel-1 ion channel that is capsaicin sensitive. Nevertheless, the activation of capsaicin-sensitive sensory neurons in naked mole-rats does not produce pain-related behavior. We show that capsaicin-sensitive nociceptors in the naked mole-rat are functionally connected to superficial dorsal horn neurons as in mice. However, the same nociceptors are also functionally connected to deep dorsal horn neurons, a connectivity that is rare in mice. The pain biology of the naked mole-rat is unique among mammals, thus the study of pain mechanisms in this unusual species can provide major insights into what constitutes "normal" mammalian nociception.

  4. Selective inflammatory pain insensitivity in the African naked mole-rat (Heterocephalus glaber.

    Directory of Open Access Journals (Sweden)

    Thomas J Park

    2008-01-01

    Full Text Available In all mammals, tissue inflammation leads to pain and behavioral sensitization to thermal and mechanical stimuli called hyperalgesia. We studied pain mechanisms in the African naked mole-rat, an unusual rodent species that lacks pain-related neuropeptides (e.g., substance P in cutaneous sensory fibers. Naked mole-rats show a unique and remarkable lack of pain-related behaviors to two potent algogens, acid and capsaicin. Furthermore, when exposed to inflammatory insults or known mediators, naked mole-rats do not display thermal hyperalgesia. In contrast, naked mole-rats do display nocifensive behaviors in the formalin test and show mechanical hyperalgesia after inflammation. Using electrophysiology, we showed that primary afferent nociceptors in naked mole-rats are insensitive to acid stimuli, consistent with the animal's lack of acid-induced behavior. Acid transduction by sensory neurons is observed in birds, amphibians, and fish, which suggests that this tranduction mechanism has been selectively disabled in the naked mole-rat in the course of its evolution. In contrast, nociceptors do respond vigorously to capsaicin, and we also show that sensory neurons express a transient receptor potential vanilloid channel-1 ion channel that is capsaicin sensitive. Nevertheless, the activation of capsaicin-sensitive sensory neurons in naked mole-rats does not produce pain-related behavior. We show that capsaicin-sensitive nociceptors in the naked mole-rat are functionally connected to superficial dorsal horn neurons as in mice. However, the same nociceptors are also functionally connected to deep dorsal horn neurons, a connectivity that is rare in mice. The pain biology of the naked mole-rat is unique among mammals, thus the study of pain mechanisms in this unusual species can provide major insights into what constitutes "normal" mammalian nociception.

  5. Traceability of pH to the Mole

    Directory of Open Access Journals (Sweden)

    Maria Filomena Camões

    2015-08-01

    Full Text Available Free acidity of aqueous solutions was initially defined in 1909 by Søren Peter Lauritz Sørensen as pH = −lgcH+ (c/mol·dm−3 or m/mol·kg−1 of the free hydrogen ions in solution, H+ soon (1910 was changed to pH = paH+ = −lgaH+, integrating the new concepts of activity, ai and activity coefficient γi, for the ionic species i under concern, H+ in this case; it is ai = −lg(miγi. Since individual ions do not exist alone in solution, primary pH values cannot be assigned solely by experimental measurements, requiring extra thermodynamic model assumptions for the activity coefficient, γH+, which has put pH in a unique situation of not being fully traceable to the International System of Units (SI. Also the concept of activity is often not felt to be as perceptible as that of concentration which may present difficulties, namely with the interpretation of data. pH measurements on unknown samples rely on calibration of the measuring setup with adequate reference pH buffers. In this work, the assignment of pH values to buffers closely matching the samples, e.g., seawater, is revisited. An approach is presented to assess the quantity pmH+ = −lgmH+ profiting from the fact that, contrary to single ion activity coefficients, mean activity coefficients,   can be assessed based on experimentally assessed quantities alone, γExp ±, thus ensuring traceability to the mole, the SI base unit for amount of substance. Compatibility between γExp ± and mean activity coefficient calculated by means of Pitzer model equations, γPtz ±, validates the model for its intended use.

  6. Assessment of Visitor Satisfaction in Mole National Park, Ghana

    Directory of Open Access Journals (Sweden)

    Conrad-J.Wuleka Kuuder

    2013-01-01

    Full Text Available Arrivals to Mole National Park (MNP, the largest in Ghana were projected by management to reach 100,000 guests by the close of2010. As at the end of December 2008, the park recorded only 16, 807 guest arrivals, the highest so far in its existence. By the close of year 2010, only 14,336 tourist arrivals were recorded registering a drop, hence an illusion in attaining the2010 set target and even subsequent years to come. This therefore gave a clue that revenue generated is not always enough to support park administration and community development. This paper explores the underlying reasons accounting for this trend by finding out tourists’ preferences in the park, the category of people who patronized the park most and sourcing guest views on what can be done to make the park more attractive. A five month period was used to elicit information from498 tourists who visited the Park employing questionnaire administration and interview schedules. The results analyzed revealed that student groups in second cycle and tertiary institutions patronized the park most on the domestic front, whilst on the foreign front, all guests contacted were educated above high school level and many of them (57% were on holiday in Ghana. The driving force (motivation behind these visits was to see animals in the wild. The most preferred wildlife species visitors came to view were elephants, monkeys, lions, buffalo and birds respectively. The recommendation is made that the road linking major cities and towns to the Park which is “rough and rugged” be rehabilitated if government needs to improve tourists’ inflow to the park.

  7. Tourism potentials of Mole National Park in Northern Ghana

    Directory of Open Access Journals (Sweden)

    Conrad Wuleka Kuuder

    2012-01-01

    Full Text Available Poor access and long distances from major cities/towns have always been major problems debarring the full utilization of nature-related touristic resources. Despite this, some adventuresome tourists still make efforts to such wildlife sanctuaries to have a feel of nature. This study explores tourism exploits at Mole National Park (the largest in Ghana which is located in the northern sector of the country. An inventory of facilities through field visits and observations were ‘exacted’ to identify different types of landforms, species of wildlife, vegetation and culture which were of touristic significance around the Park and also to have an overview of tourists’ “traffic” to the Park. With regard to data collection, the questionnaire method including personal observation were employed to obtain information from the four communities that surround the Park, the Park officials and tourists who visited the facility from April to May, 2011. The results analysed revealed that turn out was comparatively low due to the remote location of the Park including poor accessibility and low income among Ghanaians. Tourism awareness among community members was found to be high. Tourists found the Park impressive in terms of its variety in wildlife and services rendered therein. It was discovered that the Park has a high tourism potential which can be harnessed to attract both domestic and international tourists and bring socio-economic benefits to Ghana. The paper suggests that improvements in road network to and in the Park and stiffer sanctions to curb poaching were major ways to enhance tourism/recreation in the Park and making it sustainable.

  8. Fructose-driven glycolysis supports anoxia resistance in the naked mole-rat.

    Science.gov (United States)

    Park, Thomas J; Reznick, Jane; Peterson, Bethany L; Blass, Gregory; Omerbašić, Damir; Bennett, Nigel C; Kuich, P Henning J L; Zasada, Christin; Browe, Brigitte M; Hamann, Wiebke; Applegate, Daniel T; Radke, Michael H; Kosten, Tetiana; Lutermann, Heike; Gavaghan, Victoria; Eigenbrod, Ole; Bégay, Valérie; Amoroso, Vince G; Govind, Vidya; Minshall, Richard D; Smith, Ewan St J; Larson, John; Gotthardt, Michael; Kempa, Stefan; Lewin, Gary R

    2017-04-21

    The African naked mole-rat's ( Heterocephalus glaber ) social and subterranean lifestyle generates a hypoxic niche. Under experimental conditions, naked mole-rats tolerate hours of extreme hypoxia and survive 18 minutes of total oxygen deprivation (anoxia) without apparent injury. During anoxia, the naked mole-rat switches to anaerobic metabolism fueled by fructose, which is actively accumulated and metabolized to lactate in the brain. Global expression of the GLUT5 fructose transporter and high levels of ketohexokinase were identified as molecular signatures of fructose metabolism. Fructose-driven glycolytic respiration in naked mole-rat tissues avoids feedback inhibition of glycolysis via phosphofructokinase, supporting viability. The metabolic rewiring of glycolysis can circumvent the normally lethal effects of oxygen deprivation, a mechanism that could be harnessed to minimize hypoxic damage in human disease. Copyright © 2017, American Association for the Advancement of Science.

  9. Hypersensitivity to contact inhibition provides a clue to cancer resistance of naked mole-rat.

    Science.gov (United States)

    Seluanov, Andrei; Hine, Christopher; Azpurua, Jorge; Feigenson, Marina; Bozzella, Michael; Mao, Zhiyong; Catania, Kenneth C; Gorbunova, Vera

    2009-11-17

    The naked mole-rat is the longest living rodent with a maximum lifespan exceeding 28 years. In addition to its longevity, naked mole-rats have an extraordinary resistance to cancer as tumors have never been observed in these rodents. Furthermore, we show that a combination of activated Ras and SV40 LT fails to induce robust anchorage-independent growth in naked mole-rat cells, while it readily transforms mouse fibroblasts. The mechanisms responsible for the cancer resistance of naked mole-rats were unknown. Here we show that naked mole-rat fibroblasts display hypersensitivity to contact inhibition, a phenomenon we termed "early contact inhibition." Contact inhibition is a key anticancer mechanism that arrests cell division when cells reach a high density. In cell culture, naked mole-rat fibroblasts arrest at a much lower density than those from a mouse. We demonstrate that early contact inhibition requires the activity of p53 and pRb tumor suppressor pathways. Inactivation of both p53 and pRb attenuates early contact inhibition. Contact inhibition in human and mouse is triggered by the induction of p27(Kip1). In contrast, early contact inhibition in naked mole-rat is associated with the induction of p16(Ink4a). Furthermore, we show that the roles of p16(Ink4a) and p27(Kip1) in the control of contact inhibition became temporally separated in this species: the early contact inhibition is controlled by p16(Ink4a), and regular contact inhibition is controlled by p27(Kip1). We propose that the additional layer of protection conferred by two-tiered contact inhibition contributes to the remarkable tumor resistance of the naked mole-rat.

  10. The Naked Mole Rat Genome Resource : facilitating analyses of cancer and longevity-related adaptations

    OpenAIRE

    Keane, Michael; Craig, Thomas; Alfoldi, Jessica; Berlin, Aaron M; Johnson, Jeremy; Seluanov, Andrei; Gorbunova, Vera; Di Palma, Federica; Lindblad-Toh, Kerstin; Church, George M; de Magalhaes, Joao Pedro

    2014-01-01

    MOTIVATION: The naked mole rat (Heterocephalus glaber) is an exceptionally long-lived and cancer-resistant rodent native to East Africa. Although its genome was previously sequenced, here we report a new assembly sequenced by us with substantially higher N50 values for scaffolds and contigs. RESULTS: We analyzed the annotation of this new improved assembly and identified candidate genomic adaptations which may have contributed to the evolution of the naked mole rat's extraordinary traits, inc...

  11. Assessment of naked mole-rat distribution and threats in Eastern Ethiopia

    Directory of Open Access Journals (Sweden)

    Mengistu Wale

    2016-08-01

    Full Text Available Objective: To identify the distribution, threats and community attitudes towards naked molerat in Eastern Ethiopia. Methods: Data were collected through direct observation and interview and Chi-square at 95% confidence interval was used for significance test. Results: Naked mole-rat was identified in Fafan, City/Shinele, Eastern Hararghe Zone and Dire Dawa Administrative. The main threats of naked mole-rat identified were agricultural expansion, human killing and lack of awareness. From a total of 100 respondents, 92% of them considered naked mole-rat as pest as a result that 46% of them participated in direct killing. Literacy rate significantly affects the willingness to participate in the conservation of naked mole-rat (χ2 = 7.478, df = 1, P < 0.05. From a total of 26% respondents who did not show the willingness to participate in the conservation, 80.8% of them were illiterate. Conclusions: Naked mole-rat is fairly common in many of the study sites. However, rapid shift from nomadic life style to cultivation of crops and lacks of awareness were the main threats of naked mole-rat. Therefore, since there is no conservation action currently, further comprehensive study is required to design conservation plan for this species.

  12. CO2 Monitoring and Background Mole Fraction at Zhongshan Station, Antarctica

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    Yulong Sun

    2014-09-01

    Full Text Available Background CO2 mole fraction and seasonal variations, measured at Zhongshan station, Antarctica, for 2010 through 2013, exhibit the expected lowest mole fraction in March with a peak in November. Irrespective of wind direction, the mole fraction of CO2 distributes evenly after polluted air from station operations is removed from the data sets. The daily range of average CO2 mole fraction in all four seasons is small. The monthly mean CO2 mole fraction at Zhongshan station is similar to that of other stations in Antarctica, with seasonal CO2 amplitudes in the order of 384–392 µmol∙mol−1. The annual increase in recent years is about 2 µmol∙mol−1∙yr−1. There is no appreciable difference between CO2 mole fractions around the coast of Antarctica and in the interior, showing that CO2 observed in Antarctica has been fully mixed in the atmosphere as it moves from the north through the southern hemisphere.

  13. High Autophagy in the Naked Mole Rat may Play a Significant Role in Maintaining Good Health

    Directory of Open Access Journals (Sweden)

    Shanmin Zhao

    2014-02-01

    Full Text Available Background/Aims: The maximum lifespan of the naked mole rat is over 28.3 years, which exceeds that of any other rodent species, suggesting that age-related changes in its body composition and functionality are either attenuated or delayed in this extraordinarily long-lived species. However, the mechanisms underlying the aging process in this species are poorly understood. In this study, we investigated whether long-lived naked mole rats display more autophagic activity than short-lived mice. Methods: Hepatic stellate cells isolated from naked mole rats were treated with 50 nM rapamycin or 20 mM 3-methyladenine (3-MA for 12 or 24 h. Expression of the autophagy marker proteins LC3-II and beclin 1 was measured with western blotting and immunohistochemistry. The induction of apoptosis was analyzed by flow cytometry. Results: Our results demonstrate that one-day-old naked mole rats have higher levels of autophagy than one-day-old short-lived C57BL/6 mice, and that both adult naked mole rats (eight months old and adult C57BL/6 mice (eight weeks old have high basal levels of autophagy, which may be an important mechanism inhibiting aging and reducing the risk of age-related diseases. Conclusion: Here, we report that autophagy facilitated the survival of hepatic stellate cells from the naked mole rat, and that treatment with 3-MA or rapamycin increased the ratio of apoptotic cells to normal hepatic stellate cells.

  14. Blunted neuronal calcium response to hypoxia in naked mole-rat hippocampus.

    Directory of Open Access Journals (Sweden)

    Bethany L Peterson

    Full Text Available Naked mole-rats are highly social and strictly subterranean rodents that live in large communal colonies in sealed and chronically oxygen-depleted burrows. Brain slices from naked mole-rats show extreme tolerance to hypoxia compared to slices from other mammals, as indicated by maintenance of synaptic transmission under more hypoxic conditions and three fold longer latency to anoxic depolarization. A key factor in determining whether or not the cellular response to hypoxia is reversible or leads to cell death may be the elevation of intracellular calcium concentration. In the present study, we used fluorescent imaging techniques to measure relative intracellular calcium changes in CA1 pyramidal cells of hippocampal slices during hypoxia. We found that calcium accumulation during hypoxia was significantly and substantially attenuated in slices from naked mole-rats compared to slices from laboratory mice. This was the case for both neonatal (postnatal day 6 and older (postnatal day 20 age groups. Furthermore, while both species demonstrated more calcium accumulation at older ages, the older naked mole-rats showed a smaller calcium accumulation response than even the younger mice. A blunted intracellular calcium response to hypoxia may contribute to the extreme hypoxia tolerance of naked mole-rat neurons. The results are discussed in terms of a general hypothesis that a very prolonged or arrested developmental process may allow adult naked mole-rat brain to retain the hypoxia tolerance normally only seen in neonatal mammals.

  15. The naked mole-rat response to oxidative stress: just deal with it.

    Science.gov (United States)

    Lewis, Kaitlyn N; Andziak, Blazej; Yang, Ting; Buffenstein, Rochelle

    2013-10-20

    The oxidative stress theory of aging has been the most widely accepted theory of aging providing insights into why we age and die for over 50 years, despite mounting evidence from a multitude of species indicating that there is no direct relationship between reactive oxygen species (ROS) and longevity. Here we explore how different species, including the longest lived rodent, the naked mole-rat, have defied the most predominant aging theory. In the case of extremely long-lived naked mole-rat, levels of ROS production are found to be similar to mice, antioxidant defenses unexceptional, and even under constitutive conditions, naked mole-rats combine a pro-oxidant intracellular milieu with high, steady state levels of oxidative damage. Clearly, naked mole-rats can tolerate this level of oxidative stress and must have mechanisms in place to prevent its translation into potentially lethal diseases. In addition to the naked mole-rat, other species from across the phylogenetic spectrum and even certain mouse strains do not support this theory. Moreover, overexpressing or knocking down antioxidant levels alters levels of oxidative damage and even cancer incidence, but does not modulate lifespan. Perhaps, it is not oxidative stress that modulates healthspan and longevity, but other cytoprotective mechanisms that allow animals to deal with high levels of oxidative damage and stress, and nevertheless live long, relatively healthy lifespans. Studying these mechanisms in uniquely long-lived species, like the naked mole-rat, may help us tease out the key contributors to aging and longevity.

  16. Blunted neuronal calcium response to hypoxia in naked mole-rat hippocampus.

    Science.gov (United States)

    Peterson, Bethany L; Larson, John; Buffenstein, Rochelle; Park, Thomas J; Fall, Christopher P

    2012-01-01

    Naked mole-rats are highly social and strictly subterranean rodents that live in large communal colonies in sealed and chronically oxygen-depleted burrows. Brain slices from naked mole-rats show extreme tolerance to hypoxia compared to slices from other mammals, as indicated by maintenance of synaptic transmission under more hypoxic conditions and three fold longer latency to anoxic depolarization. A key factor in determining whether or not the cellular response to hypoxia is reversible or leads to cell death may be the elevation of intracellular calcium concentration. In the present study, we used fluorescent imaging techniques to measure relative intracellular calcium changes in CA1 pyramidal cells of hippocampal slices during hypoxia. We found that calcium accumulation during hypoxia was significantly and substantially attenuated in slices from naked mole-rats compared to slices from laboratory mice. This was the case for both neonatal (postnatal day 6) and older (postnatal day 20) age groups. Furthermore, while both species demonstrated more calcium accumulation at older ages, the older naked mole-rats showed a smaller calcium accumulation response than even the younger mice. A blunted intracellular calcium response to hypoxia may contribute to the extreme hypoxia tolerance of naked mole-rat neurons. The results are discussed in terms of a general hypothesis that a very prolonged or arrested developmental process may allow adult naked mole-rat brain to retain the hypoxia tolerance normally only seen in neonatal mammals.

  17. Management of pest mole crickets in Florida and Puerto Rico with a nematode and parasitic wasp

    International Nuclear Information System (INIS)

    Leppla, N.C.; Frank, J.H.; Adjei, M.B.; Vicente, N.E.

    2007-01-01

    Non-indigenous invasive mole crickets, Scapteriscus vicinus Scudder (Orthoptera: Gryllotalpidae) in Florida and S. didactylus (Latreille) (the 'changa') in Puerto Rico, are being managed with an entomopathogenic nematode, Steinernema scapterisci (Nguyen and Smart) (Rhabditida: Steinernematidae), and a parasitic wasp, Larra bicolor L. (Hymenoptera: Sphecidae). Pest mole cricket populations have declined by 95% in north central Florida since these specialist natural enemies were released and established in the 1980s. Commercial production of the nematode was initiated, nearly 70 billion were applied in 34 Florida counties, and their establishment, spread, and impact on mole crickets were monitored. The infected mole crickets dispersed the nematode rapidly, so that within 6 months these parasites were present in most of the insects trapped in experimental pastures. Three years later, mole cricket populations were reduced to acceptable levels and the bahiagrass had recovered. The nematode was released for the first time in Puerto Rico during 2001 and has persisted; the wasp was introduced in the late 1930s. The geographical distribution of the wasp is being expanded in Florida and Puerto Rico by planting plots of Spermacoce verticillata (L.), a wildflower indigenous to Puerto Rico and widely distributed in southern Florida. Pastures, sod farms, golf courses, landscapes, and vegetable farms in Florida and Puerto Rico are benefiting from biological control of invasive mole crickets. (author) [es

  18. Incidence and review of hydatidiform mole; chorioadenoma, destruens and chorio carcinoma in Mirza Kochek Khan Hospita

    Directory of Open Access Journals (Sweden)

    Ghaffari V

    1995-04-01

    Full Text Available Initial management, incidence and review of hydatidiform mole, chorioadenoma and destruens and chorio carcinoma in Mirza Kochek khan hospital department of ob.gyn and gynecology oncology of women's hospital Tehran-Iran school of medicin from September 1985 to March 1990, 209 patients received primary management for hydatidiform mole at oncology department of Mirza Kochek Khan hospital medical school of Tehran Iran. All of these women HAD complete record available for analysis, of these, 167 women bydatidiform mole, 12 patients chorioadenoma-destruens 30 patients chorio carcinoma a survey was made of factors likely to provide an important clinical perspective patient aye and the status of pregnancy were noted. 41 patient only sharp curettage and 55 patients suction curettage to remove the hydatidiform mole. 97 patient require chemo terapy, suction curettage of the uterus is clearly the best means of management in the most cases their eyperince documents that hydatidiform mole is an high risk pregnancy that requires prompt and in tensive management.

  19. Central visual system of the naked mole-rat (Heterocephalus glaber).

    Science.gov (United States)

    Crish, Samuel D; Dengler-Crish, Christine M; Catania, Kenneth C

    2006-02-01

    Naked mole-rats are fossorial rodents native to eastern Africa that spend their lives in extensive subterranean burrows where visual cues are poor. Not surprisingly, they have a degenerated eye and optic nerve, suggesting they have poor visual abilities. However, little is known about their central visual system. To investigate the organization of their central visual system, we injected a neuronal tracer into the eyes of naked mole-rats and mice to compare the neural structures mediating vision. We found that the superior colliculus and lateral geniculate nucleus were severely atrophied in the naked mole-rat. The olivary pretectal nucleus was reduced but still retained its characteristic morphology, possibly indicating a role in light detection. In addition, the suprachiasmatic nucleus is well innervated and resembles the same structure in other rodents. The naked mole-rat appears to have selectively lost structures that mediate form vision while retaining structures needed for minimal entrainment of circadian rhythms. Similar results have been reported for other mole-rat species. Taken together, these data suggest that light detection may still play an important role in the lives of these "blind" animals: most likely for circadian entrainment or setting seasonal rhythms.

  20. Unraveling the message: insights into comparative genomics of the naked mole-rat.

    Science.gov (United States)

    Lewis, Kaitlyn N; Soifer, Ilya; Melamud, Eugene; Roy, Margaret; McIsaac, R Scott; Hibbs, Matthew; Buffenstein, Rochelle

    2016-08-01

    Animals have evolved to survive, and even thrive, in different environments. Genetic adaptations may have indirectly created phenotypes that also resulted in a longer lifespan. One example of this phenomenon is the preternaturally long-lived naked mole-rat. This strictly subterranean rodent tolerates hypoxia, hypercapnia, and soil-based toxins. Naked mole-rats also exhibit pronounced resistance to cancer and an attenuated decline of many physiological characteristics that often decline as mammals age. Elucidating mechanisms that give rise to their unique phenotypes will lead to better understanding of subterranean ecophysiology and biology of aging. Comparative genomics could be a useful tool in this regard. Since the publication of a naked mole-rat genome assembly in 2011, analyses of genomic and transcriptomic data have enabled a clearer understanding of mole-rat evolutionary history and suggested molecular pathways (e.g., NRF2-signaling activation and DNA damage repair mechanisms) that may explain the extraordinarily longevity and unique health traits of this species. However, careful scrutiny and re-analysis suggest that some identified features result from incorrect or imprecise annotation and assembly of the naked mole-rat genome: in addition, some of these conclusions (e.g., genes involved in cancer resistance and hairlessness) are rejected when the analysis includes additional, more closely related species. We describe how the combination of better study design, improved genomic sequencing techniques, and new bioinformatic and data analytical tools will improve comparative genomics and ultimately bridge the gap between traditional model and nonmodel organisms.

  1. Prophylactic chemotherapy for hydatidiform mole to prevent gestational trophoblastic neoplasia.

    Science.gov (United States)

    Wang, Qiuyi; Fu, Jing; Hu, Lina; Fang, Fang; Xie, Lingxia; Chen, Hengxi; He, Fan; Wu, Taixiang; Lawrie, Theresa A

    2017-09-11

    This is an update of the original Cochrane Review published in Cochrane Library, Issue 10, 2012.Hydatidiform mole (HM), also called a molar pregnancy, is characterised by an overgrowth of foetal chorionic tissue within the uterus. HMs may be partial (PM) or complete (CM) depending on their gross appearance, histopathology and karyotype. PMs usually have a triploid karyotype, derived from maternal and paternal origins, whereas CMs are diploid and have paternal origins only. Most women with HM can be cured by evacuation of retained products of conception (ERPC) and their fertility preserved. However, in some women the growth persists and develops into gestational trophoblastic neoplasia (GTN), a malignant form of the disease that requires treatment with chemotherapy. CMs have a higher rate of malignant transformation than PMs. It may be possible to reduce the risk of GTN in women with HM by administering prophylactic chemotherapy (P-Chem). However, P-Chem given before or after evacuation of HM to prevent malignant sequelae remains controversial, as the risks and benefits of this practice are unclear. To evaluate the effectiveness and safety of P-Chem to prevent GTN in women with a molar pregnancy. To investigate whether any subgroup of women with HM may benefit more from P-Chem than others. For the original review we performed electronic searches in the Cochrane Gynaecological Cancer Specialised Register, the Cochrane Central Register of Controlled Trials (CENTRAL, Issue 2, 2012), MEDLINE (1946 to February week 4, 2012) and Embase (1980 to 2012, week 9). We developed the search strategy using free text and MeSH. For this update we searched the Cochrane Central Register of Controlled Trials (CENTRAL, Issue 5, 2017), MEDLINE (February 2012 to June week 1, 2017) and Embase (February 2012 to 2017, week 23). We also handsearched reference lists of relevant literature to identify additional studies and searched trial registries. We included randomised controlled trials

  2. Evaluation of the MoleMateTM training program for assessment of suspicious pigmented lesions in primary care

    Directory of Open Access Journals (Sweden)

    Annabel Wood

    2008-05-01

    Conclusion The MoleMateTM training program is a potentially effective and acceptable informatics tool to teach practitioners to recognise the features of SPLs identified by the MoleMateTM system. It will be used as part of the intervention in a randomised controlled trial to compare the diagnostic accuracy and appropriate referral rates of practitioners using the MoleMateTM system with best practice in primary care.

  3. Role of immunoexpression of cyclin D1, D3, retinoblastoma (Rb mutant and clinical risk factors on complete mole as risk factors of persistent mole

    Directory of Open Access Journals (Sweden)

    Yudi M Hidayat

    2015-10-01

    Full Text Available Introduction: Changes in complete hydatidiform mole (CHM that become persistent are difficult to handle because the malignant pathogenesis of CHM is still unclear. The growth of abnormal cells in CHM is thought to be caused by cell cycle abnormalities. Some components that play a role in this phase include cyclin D and retinoblastoma (Rb. The aim of our study was to determine the role of clinical risk factors, as well as cyclin D1, cyclin D3 and Rb-protein, in the occurrence of persistent moles. Materials and Method: This study involves 68 CHM cases at Dr. Hasan Sadikin Hospital from 2007–2011. The protein expression of cyclin D1, cyclin D3, and Rb were determined by immunohistochemistry. The results were analyzed by comparing the two groups of CHM that became persistent to those that returned to normal, as determined by a Mochizuki regression curve assessment. Results: 20 cases (29% of CHM became persistent and that 48 cases (71% returned to normal. Significant clinical variables were age (p 0.05. Conclusion: There is a strong relationship between clinical risk factors of age, excessive proliferation histopathology, serum βhCG levels ≥100,000 mU/mL, cyclin D1 and Rb mutations with the incidence of persistent moles after the evacuation of the CHM. We proposed a model to predict the risks of persistent moles with a cut-off point of 2.384, which can be used as a reference for patients with CHM.

  4. A Mole for Warm Magnetic and Optical Measurements of LHC Dipoles

    CERN Document Server

    Bottura, L; Deferne, G; Glöckner, C; Jansen, H; Köster, A; Legrand, P; Rijllart, A; Sievers, P

    2000-01-01

    A new rotating coil probe (a mole) has been developed for the simultaneous measurement of the magnetic field and magnetic axis of warm superconducting LHC dipoles and associated corrector windings. The mole houses a radial rotating coil and travels inside the magnet aperture by means of an externally driven two-way traction belt. The coil is rotated by an on-board piezo motor, being tested in view of future devices for cold measurements as the only type of motor compatible with strong magnetic fields. A virtual light spot is generated in the coil center by a LED source. The position of this light spot is measured from the outside by a system including a telescope, a CCD camera and a DSP. Jigs on reference granite tables are used to transfer the optical measurements to the magnet fiducials. We describe here the main characteristics and performance of the mole

  5. The molecular basis of acid insensitivity in the African naked mole-rat.

    Science.gov (United States)

    Smith, Ewan St John; Omerbašić, Damir; Lechner, Stefan G; Anirudhan, Gireesh; Lapatsina, Liudmila; Lewin, Gary R

    2011-12-16

    Acid evokes pain by exciting nociceptors; the acid sensors are proton-gated ion channels that depolarize neurons. The naked mole-rat (Heterocephalus glaber) is exceptional in its acid insensitivity, but acid sensors (acid-sensing ion channels and the transient receptor potential vanilloid-1 ion channel) in naked mole-rat nociceptors are similar to those in other vertebrates. Acid inhibition of voltage-gated sodium currents is more profound in naked mole-rat nociceptors than in mouse nociceptors, however, which effectively prevents acid-induced action potential initiation. We describe a species-specific variant of the nociceptor sodium channel Na(V)1.7, which is potently blocked by protons and can account for acid insensitivity in this species. Thus, evolutionary pressure has selected for an Na(V)1.7 gene variant that tips the balance from proton-induced excitation to inhibition of action potential initiation to abolish acid nociception.

  6. Extended longevity of reproductives appears to be common in Fukomys mole-rats (Rodentia, Bathyergidae.

    Directory of Open Access Journals (Sweden)

    Philip Dammann

    Full Text Available African mole-rats (Bathyergidae, Rodentia contain several social, cooperatively breeding species with low extrinsic mortality and unusually high longevity. All social bathyergids live in multigenerational families where reproduction is skewed towards a few breeding individuals. Most of their offspring remain as reproductively inactive "helpers" in their natal families, often for several years. This "reproductive subdivision" of mole-rat societies might be of interest for ageing research, as in at least one social bathyergid (Ansell's mole-rats Fukomys anselli, breeders have been shown to age significantly slower than non-breeders. These animals thus provide excellent conditions for studying the epigenetics of senescence by comparing divergent longevities within the same genotypes without the inescapable short-comings of inter-species comparisons. It has been claimed that many if not all social mole-rat species may have evolved similar ageing patterns, too. However, this remains unclear on account of the scarcity of reliable datasets on the subject. We therefore analyzed a 20-year breeding record of Giant mole-rats Fukomys mechowii, another social bathyergid species. We found that breeders indeed lived significantly longer than helpers (ca. 1.5-2.2fold depending on the sex, irrespective of social rank or other potentially confounding factors. Considering the phylogenetic positions of F. mechowii and F. anselli and unpublished data on a third Fukomys-species (F. damarensis showing essentially the same pattern, it seems probable that the reversal of the classic trade-off between somatic maintenance and sexual reproduction is characteristic of the whole genus and hence of the vast majority of social mole-rats.

  7. Stress Resistance in the Naked Mole-Rat: The Bare Essentials – A Mini-Review

    Science.gov (United States)

    Lewis, Kaitlyn N.; Mele, James; Hornsby, Peter J.; Buffenstein, Rochelle

    2012-01-01

    Background Studies comparing similar-sized species with disparate longevity may elucidate novel mechanisms that abrogate aging and prolong good health. We focus on the longest living rodent, the naked mole-rat. This mouse-sized mammal lives ∼8 times longer than do mice and, despite high levels of oxidative damage evident at a young age, it is not only very resistant to spontaneous neoplasia but also shows minimal decline in age-associated physiological traits. Objectives We assess the current status of stress resistance and longevity, focusing in particular on the molecular and cellular responses to cytotoxins and other stressors between the short-lived laboratory mouse and the naked mole-rat. Results Like other experimental animal models of lifespan extension, naked mole-rat fibroblasts are extremely tolerant of a broad spectrum of cytotoxins including heat, heavy metals, DNA-damaging agents and xenobiotics, showing LD50 values between 2- and 20-fold greater than those of fibroblasts of shorter-lived mice. Our new data reveal that naked mole-rat fibroblasts stop proliferating even at low doses of toxin whereas those mouse fibroblasts that survive treatment rapidly re-enter the cell cycle and may proliferate with DNA damage. Naked mole-rat fibroblasts also show significantly higher constitutive levels of both p53 and Nrf2 protein levels and activity, and this increases even further in response to toxins. Conclusion Enhanced cell signaling via p53 and Nrf2 protects cells against proliferating with damage, augments clearance of damaged proteins and organelles and facilitates the maintenance of both genomic and protein integrity. These pathways collectively regulate a myriad of mechanisms which may contribute to the attenuated aging profile and sustained healthspan of the naked mole-rat. Understanding how these are regulated may be also integral to sustaining positive human healthspan well into old age and may elucidate novel therapeutics for delaying the onset and

  8. Stress resistance in the naked mole-rat: the bare essentials - a mini-review.

    Science.gov (United States)

    Lewis, Kaitlyn N; Mele, James; Hornsby, Peter J; Buffenstein, Rochelle

    2012-01-01

    Studies comparing similar-sized species with disparate longevity may elucidate novel mechanisms that abrogate aging and prolong good health. We focus on the longest living rodent, the naked mole-rat. This mouse-sized mammal lives ~8 times longer than do mice and, despite high levels of oxidative damage evident at a young age, it is not only very resistant to spontaneous neoplasia but also shows minimal decline in age-associated physiological traits. We assess the current status of stress resistance and longevity, focusing in particular on the molecular and cellular responses to cytotoxins and other stressors between the short-lived laboratory mouse and the naked mole-rat. Like other experimental animal models of lifespan extension, naked mole-rat fibroblasts are extremely tolerant of a broad spectrum of cytotoxins including heat, heavy metals, DNA-damaging agents and xenobiotics, showing LD(50) values between 2- and 20-fold greater than those of fibroblasts of shorter-lived mice. Our new data reveal that naked mole-rat fibroblasts stop proliferating even at low doses of toxin whereas those mouse fibroblasts that survive treatment rapidly re-enter the cell cycle and may proliferate with DNA damage. Naked mole-rat fibroblasts also show significantly higher constitutive levels of both p53 and Nrf2 protein levels and activity, and this increases even further in response to toxins. Enhanced cell signaling via p53 and Nrf2 protects cells against proliferating with damage, augments clearance of damaged proteins and organelles and facilitates the maintenance of both genomic and protein integrity. These pathways collectively regulate a myriad of mechanisms which may contribute to the attenuated aging profile and sustained healthspan of the naked mole-rat. Understanding how these are regulated may be also integral to sustaining positive human healthspan well into old age and may elucidate novel therapeutics for delaying the onset and progression of physiological declines

  9. Twin pregnancy with both complete hydatiform mole and coexistent alive fetus: Case report

    Directory of Open Access Journals (Sweden)

    Achour Radhouane

    2015-12-01

    Full Text Available Twin pregnancy consisting of a complete hydatidiform mole coexisting with a live fetus is a rare condition with an incidence of 1 in 22000 to 1 in 100000 pregnancies. Clinical information is limited and management is difficult due to the risk of pregnancy complications such as fetal death, vaginal bleeding, preeclampsia, hyperthyroidism, and the risk of persistent gestational trophoblastic disease. Thus, the report described about the change of size and implantation site of the molar mass coexisting with a live fetus is rare especially about sonographic findings such as echo patterns. Recently we experienced a case of complete hydatidiform mole with a healthy infant delivered at term.

  10. Big insights from little animals: allosteric modulation and thermal sensitivity of shrew and mole hemoglobins

    DEFF Research Database (Denmark)

    Campbell, K.L.; Weber, Roy E.

    2007-01-01

    Mass-specific metabolic rate varies inversely with size, while pulmonary transit time, muscle O2 storage capacity, and the ability to exploit anaerobic metabolism vary directly with this variable. Thus, small mammals must exploit a suite of morphological and physiological adjustments to supply...... and moles. Despite these constraints, several shrew (and mole) species flourish at elevations exceeding 4500 m (where atmospheric PO2 is ... operating near their upper limits, what if any additional functional properties of the Hb of these species make these achievements possible? Our findings suggest that changes in the intrinsic O2-affinity of the Hb moiety (rather than phosphate sensitivity) underlie transitions to and from hypoxic...

  11. Influence of spatial environment on maze learning in an African mole-rat

    CSIR Research Space (South Africa)

    Du Toit, L

    2012-05-01

    Full Text Available -1 Anim Cogn DOI 10.1007/s10071-012-0503-0 Influence of spatial environment on maze learning in an African mole-rat Lydia du Toit ? Nigel C. Bennett ? Alecia Nickless ? Martin J. Whiting L. du Toit , A. Nickless , M. J. Whiting (email) School...

  12. The eye of the African mole-rat Cryptomys anselli : to see or not to see?

    NARCIS (Netherlands)

    Cernuda-Cernuda, R; Garcia-Fernandez, JM; Gordijn, MCM; Bovee-Geurts, PHM; DeGrip, WJ; de Grip, W.J.

    In an attempt to clarify its possible physiological role, we studied the eye of the Zambian mole rat Cryptomys anselli by light, electron and confocal microscopy using conventional staining as well as immunolabelling with rod and cone cell markers. The small eyes of Cryptomys are located

  13. The eye of the african mole-rat Cryptomys anselli: to see or not to see?

    NARCIS (Netherlands)

    Cernuda-Cernuda, R.; Garcia-Fernandez, J.M.; Gordijn, M.C.; Bovee-Geurts, P.H.M.; Grip, W.J. de

    2003-01-01

    In an attempt to clarify its possible physiological role, we studied the eye of the Zambian mole rat Cryptomys anselli by light, electron and confocal microscopy using conventional staining as well as immunolabelling with rod and cone cell markers. The small eyes of Cryptomys are located

  14. The reproductive biology of the giant Zambian mole-rat, Cryptomys ...

    African Journals Online (AJOL)

    1994-09-29

    Sep 29, 1994 ... gala (12° 27'S, 2T51'E) in the Copperbelt of northern Zam- hia. ... mole-rat, a pair of animals and a group comprising one male and three females .... Figure 1 Changes In bouy mass of a captive female Cr.vptom.vs mechowi ... The sex distribution was .... 'Ine social Cryptomys species are chmacteril,ed hy.

  15. Pre-evacuation hCG glycoforms in uneventful complete hydatidiform mole and persistent trophoblastic disease.

    NARCIS (Netherlands)

    Thomas, C.M.G.; Kerkmeijer, L.G.W.; Ariaens, H.J.; Steen, R. van der; Massuger, L.F.A.G.; Sweep, F.C.

    2010-01-01

    OBJECTIVE: To investigate whether the glycoform distribution patterns of human chorionic gonadotropin (hCG) obtained by chromatofocusing in pre-evacuation serum are different for patients who will eventually develop into persistent trophoblastic disease in case of complete hydatidiform mole

  16. Reading about the Power of Music: "Mole Music" and "Children of the Stone"

    Science.gov (United States)

    Cardany, Audrey Berger

    2016-01-01

    In this article, I review two books that address the power of music for the individual and group. Both books address the benefits of making, learning, and listening to music during times of conflict. The first brief review is David McPhail's picture book "Mole Music." The second is "Children of the Stone: The Power of Music in a…

  17. The Vital Role of Basic Mathematics in Teaching and Learning the Mole Concept

    Science.gov (United States)

    Mehrotra, Alka; Koul, Anjni

    2016-01-01

    This article focuses on the importance of activity-based teaching in understanding the mole concept and the vital role of basic mathematical operations. It describes needs-based training for teachers in a professional development programme in India. Analysis of test results before and after the training indicates that teachers improved their…

  18. Plasticity and constraints on social evolution in African mole-rats: ultimate and proximate factors.

    Science.gov (United States)

    Faulkes, Chris G; Bennett, Nigel C

    2013-05-19

    Here, we review comparative studies of African mole-rats (family Bathyergidae) to explain how constraints acting at the ultimate (environmental) and proximate (organismal) levels have led to convergent gains and losses of sociality within this extensive adaptive radiation of subterranean rodents endemic to sub-Saharan Africa. At the ultimate level, living in environments that range from mesic through to arid has led to both variation and flexibility in social organization among species, culminating in the pinnacle of social evolution in the eusocial naked and Damaraland mole-rats (Heterocephalus glaber and Fukomys damarensis). The common mole-rat (Cryptomys hottentotus) provides a model example of how plasticity in social traits exists within a single species inhabiting areas with different ecological constraint. At the proximate level, reproductive strategies and cooperative breeding may be constrained by the correlated evolution of a suite of traits including physiological suppression of reproduction, the development of physiological and morphological castes, and the mode of ovulatory control and seasonality in breeding. Furthermore, recent neurobiological advances indicate that differential patterns of neurotransmitter expression within the forebrain may underpin (and limit) either a solitary or group living/cooperative lifestyle not only in mole-rats, but also more widely among disparate mammalian taxa.

  19. case of natural queen succession in a captive colony of naked mole ...

    African Journals Online (AJOL)

    Naked mole-rats occur in large colonies where usually a single queen monopolizes reproduction. Queen succession occurs from within usually as a result of aggressive encounters with subordinate females that queue for reproductive succession following colony instability, which inevitably results in death of either the ...

  20. Comparative analysis of genome maintenance genes in naked mole rat, mouse, and human

    NARCIS (Netherlands)

    S.L. Macrae (Sheila L.); Q. Zhang (Quanwei); C. Lemetre (Christophe); I. Seim (Inge); R.B. Calder (Robert B.); J.H.J. Hoeijmakers (Jan); Y. Suh (Yousin); V.N. Gladyshev (Vadim N.); A. Seluanov (Andrei); V. Gorbunova (Vera); J. Vijg (Jan); Z.D. Zhang (Zhengdong D.)

    2015-01-01

    textabstractGenome maintenance (GM) is an essential defense system against aging and cancer, as both are characterized by increased genome instability. Here, we compared the copy number variation and mutation rate of 518 GM-associated genes in the naked mole rat (NMR), mouse, and human genomes. GM

  1. Morphological divergence of breeders and helpers in wild Damaraland mole-rat societies.

    Science.gov (United States)

    Young, Andrew J; Bennett, Nigel C

    2010-11-01

    The specialization of body shape to an individual's role within society represents a pinnacle of social evolution. Although commonplace among social insects, divergence in the body shapes of breeders and helpers has to date been documented in just one social vertebrate, the naked mole-rat, Heterocephalus glaber; an extraordinary species in which large colony size and frequent inbreeding may have favored the evolution of such specialization. Here, we present new evidence of morphological divergence between breeders and helpers in the Damaraland mole-rat, Fukomys damarensis; a much less socially extreme species that reflects an independent evolutionary origin of sociality. Using longitudinal data from wild populations, we show that dominant female Damaraland mole-rats, like many social insect queens, have a significantly more elongate body shape than subordinates. This difference arises not from a pre-existing difference in the body shapes of subordinates that do, and those that do not, become dominant, but from a modification to the growth trajectory of subordinates on dominance acquisition. Our findings reveal a wider role for morphological divergence within vertebrate societies and, as Damaraland mole-rats neither live in unusually large groups nor inbreed, suggest that circumstances favoring the evolution of such specializations may be more widespread among vertebrates than previously supposed. © 2010 The Author(s). Evolution© 2010 The Society for the Study of Evolution.

  2. Blunted behavioral and c Fos responses to acidic fumes in the African naked mole-rat.

    Science.gov (United States)

    LaVinka, Pamela Colleen; Park, Thomas J

    2012-01-01

    Acidosis in the skin triggers activation of pain pathways and behaviors indicative of pain in vertebrates. The exception is the naked mole-rat, the only known vertebrate to show physiological and behavioral insensitivity to acid pain in the skin. The goal of the present study was to determine behavioral and physiological responses of this species to airborne acidic fumes, which would be expected to affect the trigeminal pain pathway in other species. Behaviorally, naked mole-rats did not avoid fumes from moderately high concentrations of acetic acid (10 and 20%), and c Fos labeling showed no increase in activity in the trigeminal nuclei and nucleus tractus solitarius. In contrast, these concentrations triggered behavioral aversion and increased Fos activity in other laboratory rodents. For a very high concentration of acetic acid (50%), naked mole-rats showed significant avoidance behavior and increased Fos labeling in the nucleus tractus solitarius caudal region, which receives vagal chemosensory information. However, there was no increase in trigeminal labeling, and in fact, activity significantly decreased. This pattern is opposite of that associated with another irritant, ammonia fumes, which elicited an increase in trigeminal but not nucleus tractus solitarius Fos labeling, and no behavioral avoidance. Behavioral avoidance of acidic fumes, but no increased labeling in the trigeminal pain nucleus is consistent with the notion of adaptations to blunt acid pain, which would be advantageous for naked mole-rats as they normally live under chronically high levels of acidosis-inducing CO(2).

  3. Blunted behavioral and c Fos responses to acidic fumes in the African naked mole-rat.

    Directory of Open Access Journals (Sweden)

    Pamela Colleen LaVinka

    Full Text Available Acidosis in the skin triggers activation of pain pathways and behaviors indicative of pain in vertebrates. The exception is the naked mole-rat, the only known vertebrate to show physiological and behavioral insensitivity to acid pain in the skin. The goal of the present study was to determine behavioral and physiological responses of this species to airborne acidic fumes, which would be expected to affect the trigeminal pain pathway in other species. Behaviorally, naked mole-rats did not avoid fumes from moderately high concentrations of acetic acid (10 and 20%, and c Fos labeling showed no increase in activity in the trigeminal nuclei and nucleus tractus solitarius. In contrast, these concentrations triggered behavioral aversion and increased Fos activity in other laboratory rodents. For a very high concentration of acetic acid (50%, naked mole-rats showed significant avoidance behavior and increased Fos labeling in the nucleus tractus solitarius caudal region, which receives vagal chemosensory information. However, there was no increase in trigeminal labeling, and in fact, activity significantly decreased. This pattern is opposite of that associated with another irritant, ammonia fumes, which elicited an increase in trigeminal but not nucleus tractus solitarius Fos labeling, and no behavioral avoidance. Behavioral avoidance of acidic fumes, but no increased labeling in the trigeminal pain nucleus is consistent with the notion of adaptations to blunt acid pain, which would be advantageous for naked mole-rats as they normally live under chronically high levels of acidosis-inducing CO(2.

  4. Hydatidiform mole and fetus with normal karyotype: support of a separate entity

    DEFF Research Database (Denmark)

    Vejerslev, L O; Sunde, L; Hansen, B F

    1991-01-01

    Repetitive hydatidiform mole was observed in four pregnancies. The pregnancies presented with heavy bleeding and vomiting, but the post-evacuation courses were uncomplicated, with rapid regression of serum hCG levels. Cytogenetic investigations, analyses of restriction fragment length polymorphis...

  5. A Mole in the Sole: Case Report on Eccrine Poroma | Das | Internet ...

    African Journals Online (AJOL)

    Eccrine poroma is a benign tumor which arises from the intraepidermal portion of the eccrine sweat glands. 81 year old diabetic grandma worried about her persistently uncontrolled blood sugar owing to a painless mole in the sole of right foot for 2 years. Excisional biopsy revealed a sweat duct benign eccrine poroma.

  6. Hardness evaluation of cured urea-formaldehyde resins with different formaldehyde/urea mole ratios using nanoindentation method

    Science.gov (United States)

    Byung-Dae Park; Charles R. Frihart; Yan Yu; Adya P. Singh

    2013-01-01

    To understand the influence of formaldehyde/urea (F/U) mole ratio on the properties of urea–formaldehyde (UF) resins, this study investigated hardness of cured UF resins with different F/U mole ratios using a nanoindentation method. The traditional Brinell hardness (HB) method was also used...

  7. Biological and biochemical properties of human chorionic gonadotropin from urine of patients with hydatidiform mole and its radioimmunoassay

    International Nuclear Information System (INIS)

    Nishimura, Ryuichiro; Hamamoto, Tamotsu; Tanabe, Keizo; Takemori, Masayuki; Ashitaka, Yoshihiko

    1981-01-01

    Human chorionic gonadotropin (hCG) was extracted and purified from the urine of four patients with hydatidiform mole. The immunological activities of the hCG-hydatidiform mole by hCG radioimmunoassay (RIA) ranged from 9,380 to 9,700 IU/mg, and the biological activities measured by the immature rat ovarian weight method ranged from 7,250 to 7,780 IU/mg. The results of the amino acid compositions of all the hCG-hydatidiform moles were practically identical with those of hCG-normal pregnancies. The carbohydrate moiety of the hCG-hydatidiform mole was also suspected to be almost similar to that of hCG-normal pregnancies by the results of their in vitro and in vivo biological activities. It was demonstrated that hCG-hydatidiform mole was composed of α and β subunits (similar to a hCG-normal pregnancy) when hCG-hydatidiform mole was separated into subunits by SDS disc electrophoresis after treatment with mercaptoethanol. The RIA system of hCG-hydatidiform mole can be established. The concentrations of hCG in sera of normal pregnant women and patients with trophoblastic diseases assayed by hCG-hydatidiform mole RIA were equivalent to those obtained by a standard hCG RIA. Hence, a standard hCG-immunoassay method used in the management of trophoblastic diseases is considered reasonable so far as the immunoantigenecity of hCG is concerned. (author)

  8. Distribution of vasopressin in the brain of the eusocial naked mole-rat.

    Science.gov (United States)

    Rosen, Greta J; De Vries, Geert J; Goldman, Sharry L; Goldman, Bruce D; Forger, Nancy G

    2007-02-20

    Naked mole-rats are eusocial rodents that live in large subterranean colonies in which one queen breeds with one to three males. All other animals are nonbreeding subordinates. The external features of male and female subordinates, including their genitalia, are remarkably monomorphic, as is their behavior. Because vasopressin (VP) is associated with social behaviors and sex differences in other species, its distribution in naked mole-rats was of interest. We used immunohistochemistry to examine VP in the brains of subordinate and breeding naked mole-rats of both sexes. As in other mammals, VP-immunoreactive (-ir) somata were found in the paraventricular (PVN) and supraoptic nuclei (SON) and VP-ir projections from these nuclei ran through the internal and external zone of the median eminence. However, naked mole-rats had very few VP-ir cells in the bed nucleus of the stria terminalis (BST) and none in the suprachiasmatic nucleus (SCN); the extensive network of fine-caliber VP-ir fibers usually seen in projection sites of the BST and SCN were also absent. Equally unexpected was the abundance of large-caliber VP-ir fibers in the dorsomedial septum. VP immunoreactivity was generally similar in all groups, with the exception of VP-ir cell number in the dorsomedial hypothalamus (DMH). Breeders had a population of labeled cells in the DMH that was absent, or nearly absent, in subordinates. Future studies on the function of VP in these areas are needed to determine how the atypical distribution of VP immunoreactivity relates to eusociality and the unusual physiology of naked mole-rats.

  9. AoS28D, a proline-Xaa carboxypeptidase secreted by Aspergillus oryzae.

    Science.gov (United States)

    Salamin, Karine; Eugster, Philippe J; Jousson, Olivier; Waridel, Patrice; Grouzmann, Eric; Monod, Michel

    2017-05-01

    Prolyl peptidases of the MEROPS S28 family are of particular interest because they are key enzymes in the digestion of proline-rich peptides. A BLAST analysis of the Aspergillus oryzae genome revealed sequences coding for four proteases of the S28 family. Three of these proteases, AoS28A, AoS28B, and AoS28C, were previously characterized as acidic prolyl endopeptidases. The fourth protease, AoS28D, showed high sequence divergence with other S28 proteases and belongs to a phylogenetically distinct cluster together with orthologous proteases from other Aspergillus species. The objective of the present paper was to characterize AoS28D protease in terms of substrate specificity and activity. AoS28D produced by gene overexpression in A. oryzae and in Pichia pastoris was a 70-kDa glycoprotein with a 10-kDa sugar moiety. In contrast with other S28 proteases, AoS28D did not hydrolyze internal Pro-Xaa bonds of several tested peptides. Similarly, to human lysosomal Pro-Xaa carboxypeptidase, AoS28D demonstrated selectivity for cleaving C-terminal Pro-Xaa bonds which are resistant to carboxypeptidases of the S10 family concomitantly secreted by A. oryzae. Therefore, AoS28D could act in synergy with these enzymes during sequential degradation of a peptide from its C-terminus.

  10. Combate ao niilismo e ao totalitarismo em Camus

    Directory of Open Access Journals (Sweden)

    Emanuel Ricardo Germano

    2013-05-01

    Full Text Available A partir de uma leitura da peça Calígula, elaborada entre 1941 e 1943, procurar-se-á contribuir na restituição das preocupações éticas, políticas e filosóficas em cena no teatro de Albert Camus, analisando o sentido de sua expressão teatral no contexto de dois combates,ao niilismo filosófico e ao totalitarismo político de seu tempo. Observaremos em Calígula um importante registro do amadurecimento das leituras de duas heranças filosóficas relevantes na formação intelectual de Camus, Nietzsche e Heráclito, à luz da experiência do totalitarismo, observando e ressaltando com isso a amplitude de seu intertexto filosófico e a importância do aporte ético e político de Camus como pensador multifacetado, engajado nos enfrentamentos históricos e solicitações críticas de seu tempo.

  11. Sociality and the telencephalic distribution of corticotrophin-releasing factor, urocortin 3, and binding sites for CRF type 1 and type 2 receptors: A comparative study of eusocial naked mole-rats and solitary Cape mole-rats.

    Science.gov (United States)

    Coen, Clive W; Kalamatianos, Theodosis; Oosthuizen, Maria K; Poorun, Ravi; Faulkes, Christopher G; Bennett, Nigel C

    2015-11-01

    Various aspects of social behavior are influenced by the highly conserved corticotrophin-releasing factor (CRF) family of peptides and receptors in the mammalian telencephalon. This study has mapped and compared the telencephalic distribution of the CRF receptors, CRF1 and CRF2 , and two of their ligands, CRF and urocortin 3, respectively, in African mole-rat species with diametrically opposed social behavior. Naked mole-rats live in large eusocial colonies that are characterized by exceptional levels of social cohesion, tolerance, and cooperation in burrowing, foraging, defense, and alloparental care for the offspring of the single reproductive female. Cape mole-rats are solitary; they tolerate conspecifics only fleetingly during the breeding season. The telencephalic sites at which the level of CRF1 binding in naked mole-rats exceeds that in Cape mole-rats include the basolateral amygdaloid nucleus, hippocampal CA3 subfield, and dentate gyrus; in contrast, the level is greater in Cape mole-rats in the shell of the nucleus accumbens and medial habenular nucleus. For CRF2 binding, the sites with a greater level in naked mole-rats include the basolateral amygdaloid nucleus and dentate gyrus, but the septohippocampal nucleus, lateral septal nuclei, amygdalostriatal transition area, bed nucleus of the stria terminalis, and medial habenular nucleus display a greater level in Cape mole-rats. The results are discussed with reference to neuroanatomical and behavioral studies of various species, including monogamous and promiscuous voles. By analogy with findings in those species, we speculate that the abundance of CRF1 binding in the nucleus accumbens of Cape mole-rats reflects their lack of affiliative behavior. © 2015 Wiley Periodicals, Inc.

  12. Como responder ao momento presente?

    Directory of Open Access Journals (Sweden)

    Maria Filomena Molder

    2013-12-01

    Full Text Available http://dx.doi.org/10.5007/1984-784X.2013v13n19p13 Foi com esta pergunta — já um efeito de um primeiro encontro entre Irene Pimentel e eu própria — que decidimos desafiar colegas, estudantes e funci­onários da nossa Faculdade, FCSH (Faculdade de Ciências Sociais e Huma­nas, de outras Faculdades da Universidade Nova de Lisboa, de outras Uni­versidades e todos os interessados em con­siderar e discutir em comum aquilo que se passava em Portugal e que no anúncio da Jornada de 6 de De­zembro de 2012 se descrevia como um “processo de desmantela­mento social, económico e cultural sem precedentes — pese embora tantas compara­ções, baseadas na premissa da ‘eterna repetição’ — e cujas consequências não param de exceder as previsões dos responsáveis por esse desmantelamento”. Acedendo com todo o empenho e gratidão ao convite que me foi dirigido por Humberto Brito para fazer uma resenha da Jornada a publicar no primeiro número de Forma de Vida (saúdo a revista e o título, decidi-me, no entanto, a pôr de lado a resenha, que sob a forma de “Editorial” será em breve publi­cada no blogue Responder ao Momento Presente, entre­tanto criado, conjuntamente com os textos escritos pelos nossos convidados, com as parti­cipações de pessoas que corresponderam ao nosso apelo e ainda com contri­bui­ções que se alargaram para lá da Jornada; a que se juntará uma gravação em video, também disponível no Youtube.   Texto publicado originalmente em Forma de Vida, Lisboa, n.1, fev. 2013. Agrade­cemos à autora por permitir a republicação neste número do Boletim. [N.E.

  13. O2 binding and CO2 sensitivity in haemoglobins of subterranean African mole rats

    DEFF Research Database (Denmark)

    Weber, Roy E.; Jarvis, Jennifer U. M.; Fago, Angela

    2017-01-01

    that predictably safeguard pulmonary loading under hypoxic and hypercapnic burrow conditions. The O2 binding characteristics are discussed in relation to available information on the primary structure of Hbs from adult and developmental stages of mammals subjected to hypoxia and hypercapnia and the molecular......Inhabiting deep and sealed subterranean burrows, mole rats exhibit a remarkable suite of specializations, including eusociality (living in colonies with single breeding queens), extraordinary longevity, cancer immunity and poikilothermy, and extreme tolerance of hypoxia and hypercapnia. With little...... and 2,3-diphosphoglycerate (DPG, the major allosteric modulator of Hb-O2 affinity in red blood cells) in four social and two solitary species of African mole rats (family Bathyergidae) originating from different biomes and soil types across Central and Southern Africa. We found no consistent patterns...

  14. Visualization of mole fraction distribution of slow jet forming stably stratified field

    International Nuclear Information System (INIS)

    Fumizawa, Motoo; Hishida, Makoto

    1990-01-01

    An experimental study has been performed to investigate the behavior of flow and mass transfer in gaseous slow jet in which buoyancy force opposed the flow forming stably stratified field. The study has been performed to understand the basic features of air ingress phenomena at pipe rupture accident of the high temperature gas-cooled reactor. A displacement fringe technique was adopted in Mach-Zehnder interferometer to visualize the mole fraction distribution. As the result, the followings were obtained: (1) The stably stratified fields were formed in the vicinity of the outlet of the slow jet. The penetration distance of the stably stratified fields increased with Froude number. (2) Mass fraction distributions in the stably stratified fields were well correlated with the present model using the ramp mole velocity profile. (author)

  15. Effects of ultraviolet radiation on mole rats kidney: A histopathologic and ultrastructural study

    Directory of Open Access Journals (Sweden)

    Hüseyin Türker

    2014-04-01

    Full Text Available The purpose of this study was to realize the ultrastructural effects of ultraviolet radiation on the kidney tissue cells of mole rats (Spalax leucodon. The mole rats of 180–200 g body weight were divided into the control and radiation-trial groups. The control group was not given any radiation. The other groups were irradiated with artificially produced UVC radiation for 14, 28 and 60 days. The kidney tissue samples were prepared at the end of experiments and analyzed by the light and electron microscope. Several effects were observed in the kidney tissues cells analyzed in accordance with the dose magnitude of radiation. These results clearly show the detrimental effects of UVC radiation on kidney tissue cells in exposure periods dependent on radiation dose and exposure time.

  16. Analysis of internal doses to Mole voles inhabiting the East-Ural radioactive trace

    Energy Technology Data Exchange (ETDEWEB)

    Malinovsky, G.; Yarmoshenko, I. [Institute of Industrial Ecology UB RAS (Russian Federation); Chibiryak, M.; Vasil' ev, A. [Institute of Plant and Animal Ecology UB RAS (Russian Federation)

    2014-07-01

    Substantial task of development of approaches to radiation protection of non-human biota is investigation of relationships of exposure to dose, and dose to effects. Small mammals inhabiting territory of the East-Ural Radioactive Trace (EURT) are affected to ionizing radiation for many generations after accident at Mayak plutonium production in 1957. According to results of numerous studies a number of effects of exposure are observed. It is remarkable that the revealed effects are both negative and adaptive. In particular, the analysis of the variability of morphological structures of the axial skull and lower jaw in the population of northern mole vole (Ellobius talpinus Pall.), the burrowing rodent inhabiting the EURT, is of great interest. At the same time there is no reliable assessment of the radiation doses to these animals. Earlier we developed the approach to assess internal doses to mouse-like rodents (mice and voles) caused by incorporated {sup 90}Sr, which is the main dose contributing radionuclide at the EURT. Dose assessments are based on the results of beta-radiometry of intact bone. Routine methods for measuring the activity concentration of {sup 90}Sr in skeleton require ashing of samples, however in morphometric studies the destruction of material should be avoided: the skulls of mole voles are stored in the environmental samples depository of IPAE. Coefficients linking results of beta-radiometry of intact bone and activity concentration of {sup 90}Sr in skull of mouse was obtained basing on comparison of results of beta-radiometry of intact bone and bone ash. Obtained coefficients cannot be directly applied for calculating activity concentration of {sup 90}Sr in mole vole skulls because they are significantly larger. Therefore the additional study is required to assess proper coefficient of conversion from beta-radiometry to activity concentration of {sup 90}Sr. Developed dose assessment procedure includes application of the published values of

  17. Excited state intramolecular charge transfer reaction in binary mixtures of water and tertiary butanol (TBA): alcohol mole fraction dependence.

    Science.gov (United States)

    Pradhan, Tuhin; Ghoshal, Piue; Biswas, Ranjit

    2008-02-07

    The excited state intramolecular charge transfer reaction of 4-(1-azetidinyl)benzonitrile (P4C) has been studied in water-tertiary butanol (TBA) mixtures at different alcohol mole fractions by using steady state and time-resolved fluorescence spectroscopy. The ratio between the areas under the locally excited (LE) and charge transferred (CT) emission bands is found to exhibit a sharp rise at alcohol mole fraction approximately 0.04, a value at which several thermodynamic properties of this mixture is known to show anomalous change due to the enhancement of H-bonding network. The radiative rate associated with the LE emission also shows a maximum at this TBA mole fraction. Although the structural transition from the water-like tetrahedral network to the alcohol-like chain is reflected in the red shift of the absorption spectrum up to TBA mole fraction approximately 0.10, the emission bands (both LE and CT) show the typical nonideal alcohol mole fraction dependence at all TBA mole fractions. Quantum yield, CT radiative rate as well as transition moments also exhibit a nonideal alcohol mole fraction dependence. The time-resolved emission decay of P4C has been found to be biexponential at all TBA mole fractions, regardless of emission collection around either the LE or the CT bands. The time constant associated with the slow component (tau(slow)) shows a minimum at TBA mole fraction approximately 0.04, whereas such a minimum for the fast time constant, tau(fast) (representing the rate of LE --> CT conversion reaction) is not observed. The nonobservation of the minimum in tau(fast) might be due to the limited time resolution employed in our experiments.

  18. Locomotor Activity and Body Temperature Patterns over a Temperature Gradient in the Highveld Mole-Rat (Cryptomys hottentotus pretoriae).

    Science.gov (United States)

    Haupt, Meghan; Bennett, Nigel C; Oosthuizen, Maria K

    2017-01-01

    African mole-rats are strictly subterranean mammals that live in extensive burrow systems. High humidity levels in the burrows prevent mole-rats from thermoregulating using evaporative cooling. However, the relatively stable environment of the burrows promotes moderate temperatures and small daily temperature fluctuations. Mole-rats therefore display a relatively wide range of thermoregulation abilities. Some species cannot maintain their body temperatures at a constant level, whereas others employ behavioural thermoregulation. Here we test the effect of ambient temperature on locomotor activity and body temperature, and the relationship between the two parameters, in the highveld mole-rat. We exposed mole-rats to a 12L:12D and a DD light cycle at ambient temperatures of 30°C, 25°C and 20°C while locomotor activity and body temperature were measured simultaneously. In addition, we investigated the endogenous rhythms of locomotor activity and body temperature at different ambient temperatures. Mole-rats displayed nocturnal activity at all three ambient temperatures and were most active at 20°C, but least active at 30°C. Body temperature was highest at 30°C and lowest at 20°C, and the daily cycle was highly correlated with locomotor activity. We show that the mole-rats have endogenous rhythms for both locomotor activity and body temperature. However, the endogenous body temperature rhythm appears to be less robust compared to the locomotor activity rhythm. Female mole-rats appear to be more sensitive to temperature changes than males, increased heterothermy is evident at lower ambient temperatures, whilst males show smaller variation in their body temperatures with changing ambient temperatures. Mole-rats may rely more heavily on behavioural thermoregulation as it is more energy efficient in an already challenging environment.

  19. Factors in Early Adolescence Associated With a Mole-Prone Phenotype in Late Adolescence.

    Science.gov (United States)

    Xu, Haoming; Marchetti, Michael A; Dusza, Stephen W; Chung, Esther; Fonseca, Maira; Scope, Alon; Geller, Alan C; Bishop, Marilyn; Marghoob, Ashfaq A; Halpern, Allan C

    2017-10-01

    Nevi are important phenotypic risk factors for melanoma in adults. Few studies have examined the constitutional and behavioral factors associated with a mole-prone phenotype in adolescents. To identify host, behavioral, and dermoscopic factors in early adolescence (age, 14 years) that are associated with a mole-prone phenotype in late adolescence (age, 17 years). A prospective observational cohort study from the Study of Nevi in Children was conducted from January 1, 2009, to December 31, 2014, with a 2- to 3-year follow-up. A total of 569 students from the school system in Framingham, Massachusetts, were enrolled in the 8th or 9th grade (baseline; mean [SD] age, 14.4 [0.7] years). The overall retention rate was 73.3%, and 417 students were reassessed in the 11th grade. Mole-prone phenotype in the 11th grade, defined as total nevus count of the back and 1 randomly selected leg in the top decile of the cohort or having any nevi greater than 5 mm in diameter. Of the 417 students assessed at follow-up in the 11th grade (166 females and 251 males; mean [SD] age, 17.0 [0.4] years), 111 participants (26.6%) demonstrated a mole-prone phenotype: 69 students (62.2%) with 1 nevus greater than 5 mm in diameter, 23 students (20.7%) with total nevus count in the top decile, and 19 students (17.1%) with both characteristics. On multivariate analysis, baseline total nevus count (adjusted odds ratio, 9.08; 95% CI, 4.0-23.7; P adolescents for higher-intensity counseling about sun protection and skin self-examination.

  20. Naked mole-rat cortical neurons are resistant to acid-induced cell death

    OpenAIRE

    Husson, Zoé; Smith, Ewan S

    2018-01-01

    Abstract Regulation of brain pH is a critical homeostatic process and changes in brain pH modulate various ion channels and receptors and thus neuronal excitability. Tissue acidosis, resulting from hypoxia or hypercapnia, can activate various proteins and ion channels, among which acid-sensing ion channels (ASICs) a family of primarily Na+ permeable ion channels, which alongside classical excitotoxicity causes neuronal death. Naked mole-rats (NMRs, Heterocephalus glaber) are ...

  1. Digital dissection of the masticatory muscles of the naked mole-rat, Heterocephalus glaber (Mammalia, Rodentia

    Directory of Open Access Journals (Sweden)

    Philip G. Cox

    2014-06-01

    Full Text Available The naked mole-rat, Heterocephalus glaber, of the family Bathyergidae is a subterranean rodent that feeds on underground roots and tubers and digs extensive tunnel systems with its incisors. It is a highly unusual mammal with regard to its social structure, longevity, pain insensitivity and cancer resistance, all of which have made it the subject of a great deal of research in recent years. Yet, much of the basic anatomy of this species remains undocumented. In this paper, we describe the morphology of the jaw-closing musculature of the naked mole-rat, as revealed by contrast-enhanced micro-computed tomography. This technique uses an iodine stain to enable the imaging of soft tissues with microCT. The iodine-enhanced scans were used to create 3D reconstructions of the naked mole-rat masticatory muscles from which muscle masses were calculated. The jaw-closing musculature of Heterocephalus glaber is relatively very large compared to other rodents and is dominated by the superficial masseter, the deep masseter and the temporalis. The temporalis in particular is large for a rodent, covering the entirety of the braincase and much of the rear part of the orbit. The morphology of the masseter complex described here differs from two other published descriptions of bathyergid masticatory muscles, but is more similar to the arrangement seen in other rodent families. The zygomaticomandibularis (ZM muscle does not protrude through the infraorbital foramen on to the rostrum and thus the naked mole-rat should be considered protrogomorphous rather than hystricomorphous, and the morphology is consistent with secondarily lost hystricomorphy as has been previously suggested for Bathyergidae. Overall, the morphology of the masticatory musculature indicates a species with a high bite force and a wide gape–both important adaptations for a life dominated by digging with the incisors.

  2. Family Wide Molecular Adaptations to Underground Life in African Mole-Rats Revealed by Phylogenomic Analysis.

    Science.gov (United States)

    Davies, Kalina T J; Bennett, Nigel C; Tsagkogeorga, Georgia; Rossiter, Stephen J; Faulkes, Christopher G

    2015-12-01

    During their evolutionary radiation, mammals have colonized diverse habitats. Arguably the subterranean niche is the most inhospitable of these, characterized by reduced oxygen, elevated carbon dioxide, absence of light, scarcity of food, and a substrate that is energetically costly to burrow through. Of all lineages to have transitioned to a subterranean niche, African mole-rats are one of the most successful. Much of their ecological success can be attributed to a diet of plant storage organs, which has allowed them to colonize climatically varied habitats across sub-Saharan Africa, and has probably contributed to the evolution of their diverse social systems. Yet despite their many remarkable phenotypic specializations, little is known about molecular adaptations underlying these traits. To address this, we sequenced the transcriptomes of seven mole-rat taxa, including three solitary species, and combined new sequences with existing genomic data sets. Alignments of more than 13,000 protein-coding genes encompassed, for the first time, all six genera and the full spectrum of ecological and social variation in the clade. We detected positive selection within the mole-rat clade and along ancestral branches in approximately 700 genes including loci associated with tumorigenesis, aging, morphological development, and sociality. By combining these results with gene ontology annotation and protein-protein networks, we identified several clusters of functionally related genes. This family wide analysis of molecular evolution in mole-rats has identified a suite of positively selected genes, deepening our understanding of the extreme phenotypic traits exhibited by this group. © The Author 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  3. Aerosol Observing System (AOS) Handbook

    Energy Technology Data Exchange (ETDEWEB)

    Jefferson, A

    2011-01-17

    The Aerosol Observing System (AOS) is a suite of in situ surface measurements of aerosol optical and cloud-forming properties. The instruments measure aerosol properties that influence the earth’s radiative balance. The primary optical measurements are those of the aerosol scattering and absorption coefficients as a function of particle size and radiation wavelength and cloud condensation nuclei (CCN) measurements as a function of percent supersaturation. Additional measurements include those of the particle number concentration and scattering hygroscopic growth. Aerosol optical measurements are useful for calculating parameters used in radiative forcing calculations such as the aerosol single-scattering albedo, asymmetry parameter, mass scattering efficiency, and hygroscopic growth. CCN measurements are important in cloud microphysical models to predict droplet formation.

  4. Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber)

    DEFF Research Database (Denmark)

    Jørgensen, Kristine B.; Krogh-Jensen, Karen; Pickering, Darryl S

    2016-01-01

    The present study investigated the cholinergic system in the African naked mole-rat (Heterocephalus glaber) with focus on the muscarinic acetylcholine receptor subtypes M1 and M4. The protein sequences for the subtypes m 1–5 of the naked mole-rat were compared to that of the house mouse (Mus...... musculus) using basic local alignment search tool (BLAST). The presence and function of M1 and M4 was investigated in vivo, using the formalin test with the muscarinic receptor agonists xanomeline and VU0152100. Spinal cord tissue from the naked mole-rat was used for receptor saturation binding studies...

  5. Indium mole fraction effect on the structural and optical properties of quaternary AlInGaN epilayers

    International Nuclear Information System (INIS)

    Liu, J P; Jin, R Q; Zhang, J C; Wang, J F; Wu, M; Zhu, J J; Zhao, D G; Wang, Y T; Yang, H

    2004-01-01

    AlInGaN quaternary epilayers with varying In mole fraction were investigated using triple-axis x-ray diffraction and photoluminescence measurements. The indium compositional fluctuation is enhanced with increasing In mole fraction, whereas the mosaicity of the AlInGaN epilayers is determined through the GaN template quality. Based on the analysis of the temperature dependence of the PL peak position, it is found that the localization effect strengthens with increasing In mole fraction due to the larger fluctuations of the In distribution. Increasing the influence of the localized state results in increasing the emission intensity and FWHM with the In content

  6. Cancer resistance in the blind mole rat is mediated by concerted necrotic cell death mechanism

    Science.gov (United States)

    Gorbunova, Vera; Hine, Christopher; Tian, Xiao; Ablaeva, Julia; Gudkov, Andrei V.; Nevo, Eviatar; Seluanov, Andrei

    2012-01-01

    Blind mole rats Spalax (BMR) are small subterranean rodents common in the Middle East. BMR is distinguished by its adaptations to life underground, remarkable longevity (with a maximum documented lifespan of 21 y), and resistance to cancer. Spontaneous tumors have never been observed in spalacids. To understand the mechanisms responsible for this resistance, we examined the growth of BMR fibroblasts in vitro of the species Spalax judaei and Spalax golani. BMR cells proliferated actively for 7–20 population doublings, after which the cells began secreting IFN-β, and the cultures underwent massive necrotic cell death within 3 d. The necrotic cell death phenomenon was independent of culture conditions or telomere shortening. Interestingly, this cell behavior was distinct from that observed in another long-lived and cancer-resistant African mole rat, Heterocephalus glaber, the naked mole rat in which cells display hypersensitivity to contact inhibition. Sequestration of p53 and Rb proteins using SV40 large T antigen completely rescued necrotic cell death. Our results suggest that cancer resistance of BMR is conferred by massive necrotic response to overproliferation mediated by p53 and Rb pathways, and triggered by the release of IFN-β. Thus, we have identified a unique mechanism that contributes to cancer resistance of this subterranean mammal extremely adapted to life underground. PMID:23129611

  7. Radioimmunoassay of β sub unit HCG following hydatidiform mole or chorionic carcinoma

    International Nuclear Information System (INIS)

    Tscherne, G.; Puerstner, P.

    1979-01-01

    The radioimmunoassay of Beta Sub Unit HCG in the serum heralds a marked improvement in the diagnosis of gestational trophoblastic disease. In nine cases of hydatidiform mole and three cases of chorionic carcinoma serial examinations of the Beta Sub Unit HCG were performed. When the immunologic pregnancy test became negative following a hydatidiform mole or following treatment of chorionic carcinoma, the HCG excretion remained above detectable values for several more weeks. The decrease of Beta Sub Unit serum HCG was either linear or in fluctuations. The detection of fluctuations or a secondary rise in the Beta Sub Unit HCG titre permits the early diagnosis of invasive trophoblastic disease following hydatidiform mole or of insufficient treatment or recurrence in cases of chorionic carcinoma. Our results suggest that the optimal follow-up with Beta Sub Unit serum HCG is by weekly determinations until four consecutive determinations remained negative. This is followed by two determinations at bi-weekly intervals and thereafter monthly follow-up examinations. (orig.) 891 AJ/orig. 892 BRE [de

  8. Sex, social status, and CRF receptor densities in naked mole-rats.

    Science.gov (United States)

    Beery, Annaliese K; Bicks, Lucy; Mooney, Skyler J; Goodwin, Nastacia L; Holmes, Melissa M

    2016-02-01

    Naked mole-rats (Heterocephalus glaber) live in groups that are notable for their large size and caste structure, with breeding monopolized by a single female and a small number of males. Recent studies have demonstrated substantial differences between the brains of breeders and subordinates induced by changes in social standing. Corticotropin-releasing factor (CRF) receptors-which bind the hormone CRF as well as related peptides-are important regulators of stress and anxiety, and are emerging as factors affecting social behavior. We conducted autoradiographic analyses of CRF1 and CRF2 receptor binding densities in female and male naked mole-rats varying in breeding status. Both globally and in specific brain regions, CRF1 receptor densities varied with breeding status. CRF1 receptor densities were higher in subordinates across brain regions, and particularly in the piriform cortex and cortical amygdala. Sex differences were present in CRF2 receptor binding densities, as is the case in multiple vole species. CRF2 receptor densities were higher in females, both globally and in the cortical amygdala and lateral amygdalar nucleus. These results provide novel insights into the neurobiology of social hierarchy in naked mole-rats, and add to a growing body of work that links changes in the CRF system with social behavior. © 2015 Wiley Periodicals, Inc.

  9. Cutaneous and periodontal inputs to the cerebellum of the naked mole-rat (Heterocephalus glaber).

    Science.gov (United States)

    Sarko, Diana K; Leitch, Duncan B; Catania, Kenneth C

    2013-01-01

    The naked mole-rat (Heterocephalus glaber) is a small fossorial rodent with specialized dentition that is reflected by the large cortical area dedicated to representation of the prominent incisors. Due to naked mole-rats' behavioral reliance on the incisors for digging and for manipulating objects, as well as their ability to move the lower incisors independently, we hypothesized that expanded somatosensory representations of the incisors would be present within the cerebellum in order to accommodate a greater degree of proprioceptive, cutaneous, and periodontal input. Multiunit electrophysiological recordings targeting the ansiform lobule were used to investigate tactile inputs from receptive fields on the entire body with a focus on the incisors. Similar to other rodents, a fractured somatotopy appeared to be present with discrete representations of the same receptive fields repeated within each folium of the cerebellum. These findings confirm the presence of somatosensory inputs to a large area of the naked mole-rat cerebellum with particularly extensive representations of the lower incisors and mystacial vibrissae. We speculate that these extensive inputs facilitate processing of tactile cues as part of a sensorimotor integration network that optimizes how sensory stimuli are acquired through active exploration and in turn adjusts motor outputs (such as independent movement of the lower incisors). These results highlight the diverse sensory specializations and corresponding brain organizational schemes that have evolved in different mammals to facilitate exploration of and interaction with their environment.

  10. Cutaneous and periodontal inputs to the cerebellum of the naked mole-rat (Heterocephalus glaber

    Directory of Open Access Journals (Sweden)

    Diana K Sarko

    2013-11-01

    Full Text Available The naked mole-rat (Heterocephalus glaber is a small fossorial rodent with specialized dentition that is reflected by the large cortical area dedicated to representation of the prominent incisors. Due to naked mole-rats’ behavioral reliance on the incisors for digging and for manipulating objects, as well as their ability to move the lower incisors independently, we hypothesized that expanded somatosensory representations of the incisors would be present within the cerebellum in order to accommodate a greater degree of proprioceptive, cutaneous, and periodontal input. Multiunit electrophysiological recordings targeting the ansiform lobule were used to investigate tactile inputs from receptive fields on the entire body with a focus on the incisors. Similar to other rodents, a fractured somatotopy appeared to be present with discrete representations of the same receptive fields repeated within each folium of the cerebellum. These findings confirm the presence of somatosensory inputs to a large area of the naked mole-rat cerebellum with particularly extensive representations of the lower incisors and mystacial vibrissae. We speculate that these extensive inputs facilitate processing of tactile cues as part of a sensorimotor integration network that optimizes how sensory stimuli are acquired through active exploration and in turn adjusts motor outputs (such as independent movement of the lower incisors. These results highlight the diverse sensory specializations and corresponding brain organizational schemes that have evolved in different mammals to facilitate exploration of and interaction with their environment.

  11. Naked mole-rat mortality rates defy Gompertzian laws by not increasing with age

    Science.gov (United States)

    Ruby, J Graham; Smith, Megan

    2018-01-01

    The longest-lived rodent, the naked mole-rat (Heterocephalus glaber), has a reported maximum lifespan of >30 years and exhibits delayed and/or attenuated age-associated physiological declines. We questioned whether these mouse-sized, eusocial rodents conform to Gompertzian mortality laws by experiencing an exponentially increasing risk of death as they get older. We compiled and analyzed a large compendium of historical naked mole-rat lifespan data with >3000 data points. Kaplan-Meier analyses revealed a substantial portion of the population to have survived at 30 years of age. Moreover, unlike all other mammals studied to date, and regardless of sex or breeding-status, the age-specific hazard of mortality did not increase with age, even at ages 25-fold past their time to reproductive maturity. This absence of hazard increase with age, in defiance of Gompertz’s law, uniquely identifies the naked mole-rat as a non-aging mammal, confirming its status as an exceptional model for biogerontology. PMID:29364116

  12. Moles (Nevi)

    Science.gov (United States)

    ... Registration General information Housing & travel Education Exhibit hall Mobile app 2019 Annual Meeting Derm Exam Prep Course ... SkinPAC State societies Scope of practice Truth in advertising NP/PA laws Action center Public and patients ...

  13. Hydatidiform mole

    Science.gov (United States)

    ... avoid another pregnancy and to use a reliable contraceptive for 6 to 12 months after treatment for ... Hockberger RS, Walls RM, et al, eds. Rosen's Emergency Medicine . 8th ed. Philadelphia, PA: Elsevier Saunders; 2014: ...

  14. Population characteristics of the mole crab, Hippa adactyla Fabricius, in the intertidal sediment at Kavaratti Atoll, Lakshadweep Islands

    Digital Repository Service at National Institute of Oceanography (India)

    Ingole, B.S.; Sreepada, R.A.; Ansari, Z.A.; Parulekar, A.H.

    Some population characteristics of a little known mole crab Hippa adactyla from the sandy intertidal habitat of Kavaratti atoll, Lakshadweep islands, were studied for understanding the resource potentials besides some features of breeding behavior...

  15. Community structure variability of Uropodina mites (Acari: Mesostigmata) in nests of the common mole, Talpa europaea, in Central Europe

    OpenAIRE

    Napiera?a, Agnieszka; M?dra, Anna; Leszczy?ska-Deja, Kornelia; Gwiazdowicz, Dariusz J.; Go?dyn, Bart?omiej; B?oszyk, Jerzy

    2016-01-01

    Underground nests of Talpa europaea, known as the common mole, are very specific microhabitats, which are also quite often inhabited by various groups of arthropods. Mites from the suborder Uropodina (Acari: Mesostigmata) are only one of them. One could expect that mole nests that are closely located are inhabited by communities of arthropods with similar species composition and structure. However, results of empirical studies clearly show that even nests which are close to each other can be ...

  16. Efficacy of NETDC (New England Trophoblastic Disease Center prognostic index score to predict gestational trophoblastic tumor from hydatidiform mole

    Directory of Open Access Journals (Sweden)

    Khrismawan Khrismawan

    2004-03-01

    Full Text Available A prospective longitudinal analytic study assessing the efficacy of NETDC (New England Trophoblastic Disease Center prognostic index score in predicting malignancy after hydatidiform mole had been performed. Of the parameter evaluated; age of patients, type of hydatidiform mole, uterine enlargement, serum hCG level, lutein cyst, and presence of complicating factors were significant risk factors for malignancy after hydatidiform mole were evacuated (p<0.032. The study were done on 50 women diagnosed with hydatidiform mole with 1 year observation (January 2001-December 2002 at the Department of Obstetrics and Gynecology, Mohammad Hoesin Hospital, Palembang. The results showed that the NETDC prognostic index score predicted malignancy in 50% of high risk group and 10% in low risk group (p<0.05. This showed a higher number than that found by the WHO (19%-30%. The risk for incidence of  malignancy after hydatidiform mole in the high risk group is 9.0 times higher compared to that of the low risk group (CI: 1.769-45.786. (Med J Indones 2004; 13: 40-6 Keywords: New England Trophoblastic Disease Center (NETDC, gestational trophoblastic tumor, hydatidiform mole, high and low risk

  17. Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber).

    Science.gov (United States)

    Jørgensen, Kristine B; Krogh-Jensen, Karen; Pickering, Darryl S; Kanui, Titus I; Abelson, Klas S P

    2016-01-01

    The present study investigated the cholinergic system in the African naked mole-rat (Heterocephalus glaber) with focus on the muscarinic acetylcholine receptor subtypes M1 and M4. The protein sequences for the subtypes m 1-5 of the naked mole-rat were compared to that of the house mouse (Mus musculus) using basic local alignment search tool (BLAST). The presence and function of M1 and M4 was investigated in vivo, using the formalin test with the muscarinic receptor agonists xanomeline and VU0152100. Spinal cord tissue from the naked mole-rat was used for receptor saturation binding studies with [(3)H]-N-methylscopolamine. The BLAST test revealed 95 % protein sequence homology showing the naked mole-rat to have the genetic potential to express all five muscarinic acetylcholine receptor subtypes. A significant reduction in pain behavior was demonstrated after administration of 8.4 mg/kg in the formalin test. Administration of 50 mg/kg VU0152100 resulted in a non-significant tendency towards antinociception. The antinociceptive effects were reversed by the muscarinic acetylcholine receptor antagonist atropine. Binding studies indicated presence of muscarinic acetylcholine receptors with a radioligand affinity comparable to that reported in mice. In conclusion, muscarinic acetylcholine receptor subtypes are present in the naked mole-rat and contribute to antinociception in the naked mole-rat.

  18. Hypofunctional TrkA Accounts for the Absence of Pain Sensitization in the African Naked Mole-Rat.

    Science.gov (United States)

    Omerbašić, Damir; Smith, Ewan St J; Moroni, Mirko; Homfeld, Johanna; Eigenbrod, Ole; Bennett, Nigel C; Reznick, Jane; Faulkes, Chris G; Selbach, Matthias; Lewin, Gary R

    2016-10-11

    The naked mole-rat is a subterranean rodent lacking several pain behaviors found in humans, rats, and mice. For example, nerve growth factor (NGF), an important mediator of pain sensitization, fails to produce thermal hyperalgesia in naked mole-rats. The sensitization of capsaicin-sensitive TRPV1 ion channels is necessary for NGF-induced hyperalgesia, but naked mole-rats have fully functional TRPV1 channels. We show that exposing isolated naked mole-rat nociceptors to NGF does not sensitize TRPV1. However, the naked mole-rat NGF receptor TrkA displays a reduced ability to engage signal transduction pathways that sensitize TRPV1. Between one- and three-amino-acid substitutions in the kinase domain of the naked mole-rat TrkA are sufficient to render the receptor hypofunctional, and this is associated with the absence of heat hyperalgesia. Our data suggest that evolution has selected for a TrkA variant that abolishes a robust nociceptive behavior in this species but is still compatible with species fitness. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  19. Hypofunctional TrkA Accounts for the Absence of Pain Sensitization in the African Naked Mole-Rat

    Directory of Open Access Journals (Sweden)

    Damir Omerbašić

    2016-10-01

    Full Text Available The naked mole-rat is a subterranean rodent lacking several pain behaviors found in humans, rats, and mice. For example, nerve growth factor (NGF, an important mediator of pain sensitization, fails to produce thermal hyperalgesia in naked mole-rats. The sensitization of capsaicin-sensitive TRPV1 ion channels is necessary for NGF-induced hyperalgesia, but naked mole-rats have fully functional TRPV1 channels. We show that exposing isolated naked mole-rat nociceptors to NGF does not sensitize TRPV1. However, the naked mole-rat NGF receptor TrkA displays a reduced ability to engage signal transduction pathways that sensitize TRPV1. Between one- and three-amino-acid substitutions in the kinase domain of the naked mole-rat TrkA are sufficient to render the receptor hypofunctional, and this is associated with the absence of heat hyperalgesia. Our data suggest that evolution has selected for a TrkA variant that abolishes a robust nociceptive behavior in this species but is still compatible with species fitness.

  20. SRAO: the first southern robotic AO system

    Science.gov (United States)

    Law, Nicholas M.; Ziegler, Carl; Tokovinin, Andrei

    2016-08-01

    We present plans for SRAO, the first Southern Robotic AO system. SRAO will use AO-assisted speckle imaging and Robo-AO-heritage high efficiency observing to confirm and characterize thousands of planet candidates produced by major new transit surveys like TESS, and is the first AO system to be capable of building a comprehensive several-thousand-target multiplicity survey at sub-AU scales across the main sequence. We will also describe results from Robo-AO, the first robotic LGS-AO system. Robo-AO has observed tens of thousands of Northern targets, often using a similar speckle or Lucky-Imaging assisted mode. SRAO will be a moderate-order natural-guide-star adaptive optics system which uses an innovative photoncounting wavefront sensor and EMCCD speckle-imaging camera to guide on faint stars with the 4.1m SOAR telescope. The system will produce diffraction-limited imaging in the NIR on targets as faint as mν = 16. In AO-assisted speckle imaging mode the system will attain the 30-mas visible diffraction limit on targets at least as faint as mν = 17. The system will be the first Southern hemisphere robotic adaptive optics system, with overheads an order of magnitude smaller than comparable systems. Using Robo-AO's proven robotic AO software, SRAO will be capable of observing overheads on sub-minute scales, allowing the observation of at least 200 targets per night. SRAO will attain three times the angular resolution of the Palomar Robo-AO system in the visible.

  1. Study of atmospheric CH4 mole fractions at three WMO/GAW stations in China

    Science.gov (United States)

    Fang, Shuang-Xi; Zhou, Ling-Xi; Masarie, Kenneth A.; Xu, Lin; Rella, Chris W.

    2013-05-01

    CH4 mole fractions were continuously measured from 2009 to 2011 at three WMO/GAW stations in China (Lin'an, LAN; Longfengshan, LFS; and Waliguan, WLG) using three Cavity Ring Down Spectroscopy instruments. LAN and LFS are GAW regional measurement stations. LAN is located in China's most economically developed region, and LFS is in a rice production area (planting area > 40,000 km2). WLG is a global measurement station in remote northwest China. At LAN, high methane mole fractions are observed in all seasons. Surface winds from the northeast enhance CH4 values, with a maximum increase of 32 ± 15 ppb in summer. The peak to peak amplitude of the seasonal cycle is 77 ± 35 ppb. At LFS, the diurnal cycle amplitude is approximately constant throughout the year except summer, when a value of 196 ± 65 ppb is observed. CH4 values at LFS reach their peak in July, which is different from seasonal variations typically observed in the northern hemisphere. CH4 mole fractions at WLG show both the smallest values and the lowest variability. Maximum values occur during summer, which is different from other northern hemisphere WMO/GAW global stations. The seasonal cycle amplitude is 17 ± 11 ppb. The linear growth rates at LAN, LFS, and WLG are 8.0 ± 1.2, 7.9 ± 0.9, and 9.4 ± 0.2 ppb yr-1, respectively, which are all larger than the global mean over the same 3 year period. Results from this study attempt to improve our basic understanding of observed atmospheric CH4 in China.

  2. Genetic signatures for enhanced olfaction in the African mole-rats.

    Directory of Open Access Journals (Sweden)

    Sofia Stathopoulos

    Full Text Available The Olfactory Receptor (OR superfamily, the largest in the vertebrate genome, is responsible for vertebrate olfaction and is traditionally subdivided into 17 OR families. Recent studies characterising whole-OR subgenomes revealed a 'birth and death' model of evolution for a range of species, however little is known about fine-scale evolutionary dynamics within single-OR families. This study reports the first assessment of fine-scale OR evolution and variation in African mole-rats (Bathyergidae, a family of subterranean rodents endemic to sub-Saharan Africa. Because of the selective pressures of life underground, enhanced olfaction is proposed to be fundamental to the evolutionary success of the Bathyergidae, resulting in a highly diversified OR gene-repertoire. Using a PCR-sequencing approach, we analysed variation in the OR7 family across 14 extant bathyergid species, which revealed enhanced levels of functional polymorphisms concentrated across the receptors' ligand-binding region. We propose that mole-rats are able to recognise a broad range of odorants and that this diversity is reflected throughout their OR7 gene repertoire. Using both classic tests and tree-based methods to test for signals of selection, we investigate evolutionary forces across the mole-rat OR7 gene tree. Four well-supported clades emerged in the OR phylogeny, with varying signals of selection; from neutrality to positive and purifying selection. Bathyergid life-history traits and environmental niche-specialisation are explored as possible drivers of adaptive OR evolution, emerging as non-exclusive contributors to the positive selection observed at OR7 genes. Our results reveal unexpected complexity of evolutionary mechanisms acting within a single OR family, providing insightful perspectives into OR evolutionary dynamics.

  3. Multidimensional MRI-CT atlas of the naked mole-rat brain (Heterocephalus glaber).

    Science.gov (United States)

    Seki, Fumiko; Hikishima, Keigo; Nambu, Sanae; Okanoya, Kazuo; Okano, Hirotaka J; Sasaki, Erika; Miura, Kyoko; Okano, Hideyuki

    2013-01-01

    Naked mole-rats have a variety of distinctive features such as the organization of a hierarchical society (known as eusociality), extraordinary longevity, and cancer resistance; thus, it would be worthwhile investigating these animals in detail. One important task is the preparation of a brain atlas database that provide comprehensive information containing multidimensional data with various image contrasts, which can be achievable using a magnetic resonance imaging (MRI). Advanced MRI techniques such as diffusion tensor imaging (DTI), which generates high contrast images of fiber structures, can characterize unique morphological properties in addition to conventional MRI. To obtain high spatial resolution images, MR histology, DTI, and X-ray computed tomography were performed on the fixed adult brain. Skull and brain structures were segmented as well as reconstructed in stereotaxic coordinates. Data were also acquired for the neonatal brain to allow developmental changes to be observed. Moreover, in vivo imaging of naked mole-rats was established as an evaluation tool of live animals. The data obtained comprised three-dimensional (3D) images with high tissue contrast as well as stereotaxic coordinates. Developmental differences in the visual system were highlighted in particular by DTI. Although it was difficult to delineate optic nerves in the mature adult brain, parts of them could be distinguished in the immature neonatal brain. From observation of cortical thickness, possibility of high somatosensory system development replaced to the visual system was indicated. 3D visualization of brain structures in the atlas as well as the establishment of in vivo imaging would promote neuroimaging researches towards detection of novel characteristics of eusocial naked mole-rats.

  4. Multidimensional MRI-CT atlas of the naked mole-rat brain

    Directory of Open Access Journals (Sweden)

    Fumiko eSeki

    2013-12-01

    Full Text Available Naked mole-rats have a variety of distinctive features such as the organisation of a hierarchical society (known as eusociality, extraordinary longevity, and cancer resistance; thus, it would be worthwhile investigating these animals in detail. One important task is the preparation of a brain atlas database that provide comprehensive information containing multidimensional data with various image contrasts, which can be achievable using a magnetic resonance imaging (MRI. Advanced MRI techniques such as diffusion tensor imaging (DTI, which generates high contrast images of fibre structures, can characterise unique morphological properties in addition to conventional MRI. To obtain high spatial resolution images, MR histology, DTI, and X-ray computed tomography (CT were performed on the fixed adult brain. Skull and brain structures were segmented as well as reconstructed in stereotaxic coordinates. Data were also acquired for the neonatal brain to allow developmental changes to be observed. Moreover, in vivo imaging of naked mole-rats was established as an evaluation tool of live animals. The data obtained comprised three-dimensional (3D images with high tissue contrast as well as stereotaxic coordinates. Developmental differences in the visual system were highlighted in particular by DTI. Although it was difficult to delineate optic nerves in the mature adult brain, parts of them could be distinguished in the immature neonatal brain. From observation of cortical thickness, possibility of high somatosensory system development replaced to the visual system was indicated. 3D visualisation of brain structures in the atlas as well as the establishment of in vivo imaging would promote neuroimaging researches towards detection of novel characteristics of eusocial naked mole-rats.

  5. Naked mole-rat acid-sensing ion channel 3 forms nonfunctional homomers, but functional heteromers.

    Science.gov (United States)

    Schuhmacher, Laura-Nadine; Callejo, Gerard; Srivats, Shyam; Smith, Ewan St John

    2018-02-02

    Acid-sensing ion channels (ASICs) form both homotrimeric and heterotrimeric ion channels that are activated by extracellular protons and are involved in a wide range of physiological and pathophysiological processes, including pain and anxiety. ASIC proteins can form both homotrimeric and heterotrimeric ion channels. The ASIC3 subunit has been shown to be of particular importance in the peripheral nervous system with pharmacological and genetic manipulations demonstrating a role in pain. Naked mole-rats, despite having functional ASICs, are insensitive to acid as a noxious stimulus and show diminished avoidance of acidic fumes, ammonia, and carbon dioxide. Here we cloned naked mole-rat ASIC3 (nmrASIC3) and used a cell-surface biotinylation assay to demonstrate that it traffics to the plasma membrane, but using whole-cell patch clamp electrophysiology we observed that nmrASIC3 is insensitive to both protons and the non-proton ASIC3 agonist 2-guanidine-4-methylquinazoline. However, in line with previous reports of ASIC3 mRNA expression in dorsal root ganglia neurons, we found that the ASIC3 antagonist APETx2 reversibly inhibits ASIC-like currents in naked mole-rat dorsal root ganglia neurons. We further show that like the proton-insensitive ASIC2b and ASIC4, nmrASIC3 forms functional, proton-sensitive heteromers with other ASIC subunits. An amino acid alignment of ASIC3s between 9 relevant rodent species and human identified unique sequence differences that might underlie the proton insensitivity of nmrASIC3. However, introducing nmrASIC3 differences into rat ASIC3 (rASIC3) produced only minor differences in channel function, and replacing the nmrASIC3 sequence with that of rASIC3 did not produce a proton-sensitive ion channel. Our observation that nmrASIC3 forms nonfunctional homomers may reflect a further adaptation of the naked mole-rat to living in an environment with high-carbon dioxide levels. © 2018 by The American Society for Biochemistry and Molecular

  6. Ultrasound imaging of partial hydatidiform mole in the second trimester of pregnancy

    International Nuclear Information System (INIS)

    Jover Labiste, Marina

    2012-01-01

    The case report of a 27 year-old patient with 18 weeks gestation, admitted to the Obstetrics Department of 'Mariana Grajales Coello' Provincial Gynecology and Obstetrics Hospital of Santiago de Cuba due to high blood pressure, cephalea and pain in lower abdomen is described. Additional tests confirmed a partial hydatidiform mole, so that uterine curettage was performed and small fragments of endometrial stroma were removed for pathological study. Outpatient medical monitoring was recommended and the patient had an appointment at 21 days for controlling and evaluating her general state

  7. Predictive value of some hematological parameters for non-invasive and invasive mole pregnancies.

    Science.gov (United States)

    Abide Yayla, Cigdem; Özkaya, Enis; Yenidede, Ilter; Eser, Ahmet; Ergen, Evrim Bostancı; Tayyar, Ahter Tanay; Şentürk, Mehmet Baki; Karateke, Ates

    2018-02-01

    The aim of this study was to discriminate mole pregnancies and invasive forms among cases with first trimester vaginal bleeding by the utilization of some complete blood count parameters conjunct to sonographic findings and beta human chorionic gonadotropin concentration. Consecutive 257 cases with histopathologically confirmed mole pregnancies and 199 women without mole pregnancy presented with first trimester vaginal bleeding who admitted to Zeynep Kamil Women and Children's Health Training Hospital between January 2012 and January 2016 were included in this cross-sectional study. The serum beta HCG level at presentation, and beta hCG levels at 1st, 2nd and 3rd weeks of postevacuation with some parameters of complete blood count were utilized to discriminate cases with molar pregnancy and cases with invasive mole among first trimester pregnants presented with vaginal bleeding and abnormal sonographic findings. Levels of beta hCG at baseline (AUC = 0.700, p < 0.05) and 1st (AUC = 0.704, p < 0.05), 2nd (AUC = 0.870, p < 0.001) and 3rd (AUC = 0.916, p < 0.001) weeks of postevacuation period were significant predictors for the cases with persistent disease. While area under curve for mean platelet volume is 0.715, it means that mean platelet volume has 21.5% additional diagnostic value for predicting persistency in molar patients. For 8.55 cut-off point for mean platelet volume, sensitivity is 84.6% and specificity is 51.6%. Area under curve for platelet/lymphocyte ratio is 0.683 means that platelet/lymphocyte ratio has additional 18.3% diagnostic value. For 102.25 cut-off point sensitivity is 86.6% and specificity is 46.2. Simple, widely available complete blood count parameters may be used as an adjunct to other risk factors to diagnose molar pregnancies and predict postevacuation trophoblastic disease.

  8. Absence of histamine-induced itch in the African naked mole-rat and "rescue" by Substance P.

    Science.gov (United States)

    Smith, Ewan St John; Blass, Gregory R C; Lewin, Gary R; Park, Thomas J

    2010-05-24

    Recent research has proposed a pathway in which sensory neurons expressing the capsaicin activated ion channel TRPV1 are required for histamine-induced itch and subsequent scratching behavior. We examined histamine-induced itch in the African naked mole-rat (Heterocephalus glaber) and found that although naked mole-rats display innate scratching behavior, histamine was unable to evoke increased scratching as is observed in most mouse strains. Using calcium imaging, we examined the histamine sensitivity of naked mole-rat dorsal root ganglia (DRG) neurons and identified a population of small diameter neurons activated by histamine, the majority of which are also capsaicin-sensitive. This suggested that naked mole-rat sensory neurons are activated by histamine, but that spinal dorsal horn processing of sensory information is not the same as in other rodents. We have previously shown that naked mole-rats naturally lack substance P (SP) in cutaneous C-fibers, but that the neurokinin-1 receptor is expressed in the superficial spinal cord. This led us to investigate if SP deficiency plays a role in the lack of histamine-induced scratching in this species. After intrathecal administration of SP into the spinal cord we observed robust scratching behavior in response to histamine injection. Our data therefore support a model in which TRPV1-expressing sensory neurons are important for histamine-induced itch. In addition, we demonstrate a requirement for active, SP-induced post-synaptic drive to enable histamine sensitive afferents to drive itch-related behavior in the naked mole-rat. These results illustrate that it is altered dorsal horn connectivity of nociceptors that underlies the lack of itch and pain-related behavior in the naked mole-rat.

  9. Absence of histamine-induced itch in the African naked mole-rat and "rescue" by Substance P

    Directory of Open Access Journals (Sweden)

    Lewin Gary R

    2010-05-01

    Full Text Available Abstract Recent research has proposed a pathway in which sensory neurons expressing the capsaicin activated ion channel TRPV1 are required for histamine-induced itch and subsequent scratching behavior. We examined histamine-induced itch in the African naked mole-rat (Heterocephalus glaber and found that although naked mole-rats display innate scratching behavior, histamine was unable to evoke increased scratching as is observed in most mouse strains. Using calcium imaging, we examined the histamine sensitivity of naked mole-rat dorsal root ganglia (DRG neurons and identified a population of small diameter neurons activated by histamine, the majority of which are also capsaicin-sensitive. This suggested that naked mole-rat sensory neurons are activated by histamine, but that spinal dorsal horn processing of sensory information is not the same as in other rodents. We have previously shown that naked mole-rats naturally lack substance P (SP in cutaneous C-fibers, but that the neurokinin-1 receptor is expressed in the superficial spinal cord. This led us to investigate if SP deficiency plays a role in the lack of histamine-induced scratching in this species. After intrathecal administration of SP into the spinal cord we observed robust scratching behavior in response to histamine injection. Our data therefore support a model in which TRPV1-expressing sensory neurons are important for histamine-induced itch. In addition, we demonstrate a requirement for active, SP-induced post-synaptic drive to enable histamine sensitive afferents to drive itch-related behavior in the naked mole-rat. These results illustrate that it is altered dorsal horn connectivity of nociceptors that underlies the lack of itch and pain-related behavior in the naked mole-rat.

  10. MOLE: A Multidisciplinary Observatory and Laboratory of Experiments in Central Italy

    Directory of Open Access Journals (Sweden)

    Georg Dresen

    2009-03-01

    Full Text Available The structure and mechanics of active Low Angle Normal Faults (LANFs have for decades b s been posing questions—in particular, if low angle normal faults accommodate crustal extension, and if they generate large magnitude earthquakes, or if they move aseismically. To shed new light on these challenging questions, MOLE intends to drill (down to 4–5 km an active LANF in the Umbria-Marche sector of the northern Apennines (Fig. 1 and to establish a deep borehole observatory. The target site offers a unique opportunity to reach a LANF at drillable seismogenic depth to unravel the “low angle normal fault mechanical paradox” (Wernicke, 1995; Axen, 2007. In order to discuss the scientific background and plan the MOLE project, ixty-two scientists from various research fields attended an international workshop in Spoleto, Italy, on 5–8 May 2008. The workshop focused on the following goals that need to be achieved: (I to collect new observational data at depth for constraining the fault zone structure; (II to perform laboratory experiments with gouge and fault zone materials to understand frictional properties and weakeningmechanisms; (III to record microearthquakes at distance comparable to the source radius, and (IV to obtain stress and strain measurements and geochemical data in and near the fault zone at depth to understand the mechanics of earthquakes and faulting.

  11. High accuracy measurements of dry mole fractions of carbon dioxide and methane in humid air

    Directory of Open Access Journals (Sweden)

    C. W. Rella

    2013-03-01

    Full Text Available Traditional techniques for measuring the mole fractions of greenhouse gases in the well-mixed atmosphere have required dry sample gas streams (dew point inter-laboratory compatibility goals (WMO, 2011a without drying the sample gas. In this paper, we present laboratory methodology for empirically deriving the water vapour correction factors, and we summarise a series of in-situ validation experiments comparing the measurements in humid gas streams to well-characterised dry-gas measurements. By using the manufacturer-supplied correction factors, the dry-mole fraction measurements have been demonstrated to be well within the GAW compatibility goals up to a water vapour concentration of at least 1%. By determining the correction factors for individual instruments once at the start of life, this water vapour concentration range can be extended to at least 2% over the life of the instrument, and if the correction factors are determined periodically over time, the evidence suggests that this range can be extended up to and even above 4% water vapour concentrations.

  12. Dominance and queen succession in captive colonies of the eusocial naked mole-rat, Heterocephalus glaber.

    Science.gov (United States)

    Clarke, F M; Faulkes, C G

    1997-07-22

    Naked mole-rat colonies exhibit a high reproductive skew, breeding being typically restricted to one female (the 'queen') and one to three males. Other colony members are reproductively suppressed, although this suppression can be reversed following the removal or death of the queen. We examined dominance and queen succession within captive colonies to investigate the relationship between urinary testosterone and cortisol, dominance rank and reproductive status; and to determine if behavioural and/or physiological parameters can be used as predictors of queen succession. Social structure was characterized by a linear dominance hierarchy before and after queen removal. Prior to queen removal, dominance rank was negatively correlated with body weight and urinary testosterone and cortisol titres in males and females. Queen removal results in social instability and aggression between high ranking individuals. Dominance rank appears to be a good predictor of reproductive status: queens are the highest ranking colony females and are succeeded by the next highest ranking females. The intense dominance-related aggression that accompanies reproductive succession in naked mole-rats provides empirical support for optimal skew theory.

  13. Adaptations to a Subterranean Environment and Longevity Revealed by the Analysis of Mole Rat Genomes

    Directory of Open Access Journals (Sweden)

    Xiaodong Fang

    2014-09-01

    Full Text Available Subterranean mammals spend their lives in dark, unventilated environments that are rich in carbon dioxide and ammonia and low in oxygen. Many of these animals are also long-lived and exhibit reduced aging-associated diseases, such as neurodegenerative disorders and cancer. We sequenced the genome of the Damaraland mole rat (DMR, Fukomys damarensis and improved the genome assembly of the naked mole rat (NMR, Heterocephalus glaber. Comparative genome analyses, along with the transcriptomes of related subterranean rodents, revealed candidate molecular adaptations for subterranean life and longevity, including a divergent insulin peptide, expression of oxygen-carrying globins in the brain, prevention of high CO2-induced pain perception, and enhanced ammonia detoxification. Juxtaposition of the genomes of DMR and other more conventional animals with the genome of NMR revealed several truly exceptional NMR features: unusual thermogenesis, an aberrant melatonin system, pain insensitivity, and unique processing of 28S rRNA. Together, these genomes and transcriptomes extend our understanding of subterranean adaptations, stress resistance, and longevity.

  14. Androgen receptor distribution in the social decision-making network of eusocial naked mole-rats.

    Science.gov (United States)

    Holmes, Melissa M; Van Mil, Spencer; Bulkowski, Camila; Goldman, Sharry L; Goldman, Bruce D; Forger, Nancy G

    2013-11-01

    Naked mole-rats are highly social rodents that live in large groups and exhibit a strict reproductive and social hierarchy. Only a few animals in each colony breed; the remainder are non-reproductive and are socially subordinate to breeders. We have examined androgen receptor immunoreactive (AR+) cells in brain regions comprising the recently described social decision-making network in subordinate and breeder naked mole-rats of both sexes. We find that subordinates have a significantly higher percentage of AR+ cells in all brain regions expressing this protein. By contrast, there were no significant effects of sex and no sex-by-status interactions on the percentage of AR+ cells. Taken together with previous findings, the present data complete a systematic assessment of the distribution of AR protein in the social decision-making network of the eusocial mammalian brain and demonstrate a significant role for social status in the regulation of this protein throughout many nodes of this network. Copyright © 2013 Elsevier B.V. All rights reserved.

  15. Differential effects of chronic fluoxetine on the behavior of dominant and subordinate naked mole-rats.

    Science.gov (United States)

    Mongillo, Daniel L; Kosyachkova, Ekaterina A; Nguyen, Tam M; Holmes, Melissa M

    2014-01-01

    Naked mole-rats are eusocial rodents that live in large subterranean colonies with a strict reproductive and social hierarchy. The breeding female (referred to as the queen) and 1 to 3 breeding males are the only reproductive members of the colony. Breeders are socially dominant and all other colony members are non-reproductive subordinates. The effects of manipulating the serotonergic neurotransmitter system on aggression and dominance behaviors are well studied in many species, but not in eusocial rodents like the naked mole-rat. The current study investigated how the serotonergic system influences aggressive/dominant behaviors in this species. To do this, two separate but related experiments were conducted: the effects of fluoxetine hydrochloride (FLX) on status-specific behaviors of subordinates (Experiment 1) and dominant queens (Experiment 2) were evaluated both in-colony and in a social-pairing paradigm. In accordance with our main hypothesis, chronic treatment of FLX attenuated the frequency and duration of aggression in queens, but not subordinates, when paired with an unfamiliar conspecific. Further exploration of pharmacological manipulation on status-specific behaviors of this eusocial species may elucidate the neurobiological mechanisms underlying their unique and rigid social hierarchy. Copyright © 2013 Elsevier B.V. All rights reserved.

  16. Retinal ganglion cell survival and axon regeneration after optic nerve injury in naked mole-rats.

    Science.gov (United States)

    Park, Kevin K; Luo, Xueting; Mooney, Skyler J; Yungher, Benjamin J; Belin, Stephane; Wang, Chen; Holmes, Melissa M; He, Zhigang

    2017-02-01

    In the adult mammalian central nervous system (CNS), axonal damage often triggers neuronal cell death and glial activation, with very limited spontaneous axon regeneration. In this study, we performed optic nerve injury in adult naked mole-rats, the longest living rodent, with a maximum life span exceeding 30 years, and found that injury responses in this species are quite distinct from those in other mammalian species. In contrast to what is seen in other mammals, the majority of injured retinal ganglion cells (RGCs) survive with relatively high spontaneous axon regeneration. Furthermore, injured RGCs display activated signal transducer and activator of transcription-3 (STAT3), whereas astrocytes in the optic nerve robustly occupy and fill the lesion area days after injury. These neuron-intrinsic and -extrinsic injury responses are reminiscent of those in "cold-blooded" animals, such as fish and amphibians, suggesting that the naked mole-rat is a powerful model for exploring the mechanisms of neuronal injury responses and axon regeneration in mammals. J. Comp. Neurol. 525:380-388, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  17. Endocrine function and neurobiology of the longest-living rodent, the naked mole-rat.

    Science.gov (United States)

    Edrey, Yael H; Park, Thomas J; Kang, Hyesin; Biney, Adriana; Buffenstein, Rochelle

    2011-01-01

    Animals that have evolved exceptional capabilities, such as extraordinary longevity may reveal pertinent and potentially critical insights into biomedical research that are not readily apparent in standard laboratory animals. Naked mole-rats (Heterocephalus glaber; NMRs) are extremely long-lived (30 years) mouse-sized rodents. They clearly have evolved superior anti-aging mechanisms as evident by the markedly attenuated age-related decline in physiological function, sustained reproductive capacity and pronounced cancer resistance throughout their long-lives. These eusocial rodents, like the social insects, live in colonies with breeding restricted to one female and a few males. Subordinates are sexually monomorphic, yet retain the ability to become breeders, and can undergo growth surges and neural modifications at any time throughout their life. This plasticity in physiological and behavioral aspects may have contributed to their long-lives. Naked mole-rats show numerous adaptations to life underground including extreme tolerance of hypoxia, acid insensitivity, as well as independence of photoendocrine systems. Here we review what is known about their unique social structure, sensory systems, endocrinology and neurobiology, and highlight areas that may be pertinent to biogerontology. Copyright © 2010 Elsevier Inc. All rights reserved.

  18. Resonant spin-flavor conversion of supernova neutrinos: Dependence on electron mole fraction

    International Nuclear Information System (INIS)

    Yoshida, Takashi; Takamura, Akira; Kimura, Keiichi; Yokomakura, Hidekazu; Kawagoe, Shio; Kajino, Toshitaka

    2009-01-01

    Detailed dependence of resonant spin-flavor (RSF) conversion of supernova neutrinos on electron mole fraction Y e is investigated. Supernova explosion forms a hot-bubble and neutrino-driven wind region of which electron mole fraction exceeds 0.5 in several seconds after the core collapse. When a higher resonance of the RSF conversion is located in the innermost region, flavor change of the neutrinos strongly depends on the sign of 1-2Y e . At an adiabatic high RSF resonance the flavor conversion of ν e ↔ν μ,τ occurs in Y e e >0.5 and inverted mass hierarchy. In other cases of Y e values and mass hierarchies, the conversion of ν e ↔ν μ,τ occurs. The final ν e spectrum is evaluated in the cases of Y e e >0.5 taking account of the RSF conversion. Based on the obtained result, time variation of the event number ratios of low ν e energy to high ν e energy is discussed. In normal mass hierarchy, an enhancement of the event ratio should be seen in the period when the electron fraction in the innermost region exceeds 0.5. In inverted mass hierarchy, on the other hand, a dip of the event ratio should be observed. Therefore, the time variation of the event number ratio is useful to investigate the effect of the RSF conversion.

  19. Actinomicose cutânea primária do pé simulando neoplasia de partes moles: relato de caso Primary cutaneous actinomycosis of the foot mimicking a soft tissue neoplasm: a case report

    Directory of Open Access Journals (Sweden)

    Renata La Rocca Vieira

    2003-08-01

    Full Text Available Relatamos um caso de actinomicose cutânea primária do pé simulando neoplasia de partes moles, com revisão da literatura sobre sua incidência, aspectos clínicos, patológicos e de imagem. Utilizamos radiografias simples, ressonância magnética e estudo anatomopatológico. A importância do estudo se deve à raridade da enfermidade, localização atípica e, particularmente, ao diagnóstico diferencial com neoplasia de partes moles.We report a case of a patient with primary cutaneous actinomycosis of the foot mimicking a soft tissue neoplasm. A literature review on the incidence, clinical features, pathology and imaging findings is also presented. The plain films and magnetic resonance imaging findings and the pathology results are presented. This paper reports a rare disease occurring in an atypical location, simulating a soft tissue neoplasm.

  20. A stable algorithm for calculating phase equilibria with capillarity at specified moles, volume and temperature using a dynamic model

    KAUST Repository

    Kou, Jisheng

    2017-09-30

    Capillary pressure can significantly affect the phase properties and flow of liquid-gas fluids in porous media, and thus, the phase equilibrium calculation incorporating capillary pressure is crucial to simulate such problems accurately. Recently, the phase equilibrium calculation at specified moles, volume and temperature (NVT-flash) becomes an attractive issue. In this paper, capillarity is incorporated into the phase equilibrium calculation at specified moles, volume and temperature. A dynamical model for such problem is developed for the first time by using the laws of thermodynamics and Onsager\\'s reciprocal principle. This model consists of the evolutionary equations for moles and volume, and it can characterize the evolutionary process from a non-equilibrium state to an equilibrium state in the presence of capillarity effect at specified moles, volume and temperature. The phase equilibrium equations are naturally derived. To simulate the proposed dynamical model efficiently, we adopt the convex-concave splitting of the total Helmholtz energy, and propose a thermodynamically stable numerical algorithm, which is proved to preserve the second law of thermodynamics at the discrete level. Using the thermodynamical relations, we derive a phase stability condition with capillarity effect at specified moles, volume and temperature. Moreover, we propose a stable numerical algorithm for the phase stability testing, which can provide the feasible initial conditions. The performance of the proposed methods in predicting phase properties under capillarity effect is demonstrated on various cases of pure substance and mixture systems.

  1. Community structure variability of Uropodina mites (Acari: Mesostigmata) in nests of the common mole, Talpa europaea, in Central Europe.

    Science.gov (United States)

    Napierała, Agnieszka; Mądra, Anna; Leszczyńska-Deja, Kornelia; Gwiazdowicz, Dariusz J; Gołdyn, Bartłomiej; Błoszyk, Jerzy

    2016-04-01

    Underground nests of Talpa europaea, known as the common mole, are very specific microhabitats, which are also quite often inhabited by various groups of arthropods. Mites from the suborder Uropodina (Acari: Mesostigmata) are only one of them. One could expect that mole nests that are closely located are inhabited by communities of arthropods with similar species composition and structure. However, results of empirical studies clearly show that even nests which are close to each other can be different both in terms of the species composition and abundance of Uropodina communities. So far, little is known about the factors that can cause these differences. The major aim of this study was to identify factors determining species composition, abundance, and community structure of Uropodina communities in mole nests. The study is based on material collected during a long-term investigation conducted in western parts of Poland. The results indicate that the two most important factors influencing species composition and abundance of Uropodina communities in mole nests are nest-building material and depth at which nests are located. Composition of Uropodina communities in nests of moles was also compared with that of other microhabitats (e.g. rotten wood, forest litter, soil) based on data from 4421 samples collected in Poland. Communities of this habitat prove most similar to these of open areas, especially meadows, as well as some forest types.

  2. Customer satisfaction and response to AOS

    Science.gov (United States)

    1999-01-01

    Passenger surveys were conducted in each of three successive springs in order to track passenger perception of changes in service quality during AOS implementation. In general, no improvement in passenger perceptions was observed relative to a high b...

  3. Antioxidant activity and prevention of pork meat lipid oxidation using traditional Mexican condiments (pasilla dry pepper, achiote, and mole sauce

    Directory of Open Access Journals (Sweden)

    Emilio Alvarez-Parrilla

    2014-06-01

    Full Text Available Considering the extensive use of hot peppers and spicy sauces in the Mexican cuisine, in the present paper, three widely consumed Mexican condiments (mole sauce, achiote, and pasilla hot pepper were analyzed for their total phenols, flavonoids and proanthocyanidins, antioxidant activity, and protective effect against lipid oxidation in chopped pork meat. All samples were extracted first with methanol and then with acetone, and the extracts were compared. Pasilla pepper showed the highest phenolic and flavonoid content in both solvents, followed by mole and achiote. Achiote showed the highest proanthocyanidin concentration. All samples showed high antioxidant activity, and good correlations with phenolic compounds and flavonoids, while no correlation was observed in the case of condensed tannins. Mole sauce methanolic extract showed the highest inhibition of pork meat oxidation, followed by pasilla pepper, and finally achiote paste extracts. These results suggest that these condiments are useful to prevent meat lipid oxidation during storage.

  4. Current transport mechanism in graphene/AlGaN/GaN heterostructures with various Al mole fractions

    Science.gov (United States)

    Pandit, Bhishma; Seo, Tae Hoon; Ryu, Beo Deul; Cho, Jaehee

    2016-06-01

    The current transport mechanism of graphene formed on AlxGa1-xN/GaN heterostructures with various Al mole fractions (x = 0.15, 0.20, 0.30, and 0.40) is investigated. The current-voltage measurement from graphene to AlGaN/GaN shows an excellent rectifying property. The extracted Schottky barrier height of the graphene/AlGaN/GaN contacts increases with the Al mole fraction in AlGaN. However, the current transport mechanism deviates from the Schottky-Mott theory owing to the deterioration of AlGaN crystal quality at high Al mole fractions confirmed by reverse leakage current measurement.

  5. Current transport mechanism in graphene/AlGaN/GaN heterostructures with various Al mole fractions

    Directory of Open Access Journals (Sweden)

    Bhishma Pandit

    2016-06-01

    Full Text Available The current transport mechanism of graphene formed on AlxGa1−xN/GaN heterostructures with various Al mole fractions (x = 0.15, 0.20, 0.30, and 0.40 is investigated. The current–voltage measurement from graphene to AlGaN/GaN shows an excellent rectifying property. The extracted Schottky barrier height of the graphene/AlGaN/GaN contacts increases with the Al mole fraction in AlGaN. However, the current transport mechanism deviates from the Schottky-Mott theory owing to the deterioration of AlGaN crystal quality at high Al mole fractions confirmed by reverse leakage current measurement.

  6. Diagnostic reproducibility of hydatidiform moles: ancillary techniques (p57 immunohistochemistry and molecular genotyping) improve morphologic diagnosis.

    Science.gov (United States)

    Vang, Russell; Gupta, Mamta; Wu, Lee-Shu-Fune; Yemelyanova, Anna V; Kurman, Robert J; Murphy, Kathleen M; Descipio, Cheryl; Ronnett, Brigitte M

    2012-03-01

    Distinction of hydatidiform moles (HMs) from nonmolar specimens (NMs) and subclassification of HMs as complete hydatidiform moles (CHMs) and partial hydatidiform moles (PHMs) are important for clinical practice and investigational studies; yet, diagnosis based solely on morphology is affected by interobserver variability. Molecular genotyping can distinguish these entities by discerning androgenetic diploidy, diandric triploidy, and biparental diploidy to diagnose CHMs, PHMs, and NMs, respectively. Eighty genotyped cases (27 CHMs, 27 PHMs, and 26 NMs) were selected from a series of 200 potentially molar specimens previously diagnosed using p57 immunostaining and genotyping. Cases were classified by 3 gynecologic pathologists on the basis of H&E slides (masked to p57 immunostaining and genotyping results) into 1 of 3 categories (CHM, PHM, or NM) during 2 diagnostic rounds; a third round incorporating p57 immunostaining results was also conducted. Consensus diagnoses (those rendered by 2 of 3 pathologists) were determined. Genotyping results were used as the gold standard for assessing diagnostic performance. Sensitivity of a diagnosis of CHM ranged from 59% to 100% for individual pathologists and from 70% to 81% by consensus; specificity ranged from 91% to 96% for individuals and from 94% to 98% by consensus. Sensitivity of a diagnosis of PHM ranged from 56% to 93% for individual pathologists and from 70% to 78% by consensus; specificity ranged from 58% to 92% for individuals and from 74% to 85% by consensus. The percentage of correct classification of all cases by morphology ranged from 55% to 75% for individual pathologists and from 70% to 75% by consensus. The κ values for interobserver agreement ranged from 0.59 to 0.73 (moderate to good) for a diagnosis of CHM, from 0.15 to 0.43 (poor to moderate) for PHM, and from 0.13 to 0.42 (poor to moderate) for NM. The κ values for intraobserver agreement ranged from 0.44 to 0.67 (moderate to good). Addition of the p57

  7. Estimating regional methane surface fluxes: the relative importance of surface and GOSAT mole fraction measurements

    Directory of Open Access Journals (Sweden)

    A. Fraser

    2013-06-01

    Full Text Available We use an ensemble Kalman filter (EnKF, together with the GEOS-Chem chemistry transport model, to estimate regional monthly methane (CH4 fluxes for the period June 2009–December 2010 using proxy dry-air column-averaged mole fractions of methane (XCH4 from GOSAT (Greenhouse gases Observing SATellite and/or NOAA ESRL (Earth System Research Laboratory and CSIRO GASLAB (Global Atmospheric Sampling Laboratory CH4 surface mole fraction measurements. Global posterior estimates using GOSAT and/or surface measurements are between 510–516 Tg yr−1, which is less than, though within the uncertainty of, the prior global flux of 529 ± 25 Tg yr−1. We find larger differences between regional prior and posterior fluxes, with the largest changes in monthly emissions (75 Tg yr−1 occurring in Temperate Eurasia. In non-boreal regions the error reductions for inversions using the GOSAT data are at least three times larger (up to 45% than if only surface data are assimilated, a reflection of the greater spatial coverage of GOSAT, with the two exceptions of latitudes >60° associated with a data filter and over Europe where the surface network adequately describes fluxes on our model spatial and temporal grid. We use CarbonTracker and GEOS-Chem XCO2 model output to investigate model error on quantifying proxy GOSAT XCH4 (involving model XCO2 and inferring methane flux estimates from surface mole fraction data and show similar resulting fluxes, with differences reflecting initial differences in the proxy value. Using a series of observing system simulation experiments (OSSEs we characterize the posterior flux error introduced by non-uniform atmospheric sampling by GOSAT. We show that clear-sky measurements can theoretically reproduce fluxes within 10% of true values, with the exception of tropical regions where, due to a large seasonal cycle in the number of measurements because of clouds and aerosols, fluxes are within 15% of true fluxes. We evaluate our

  8. Variação anatômica radial do lenho de açoita-cavalo (Luehea divaricata e sua influência na densidade aparente. Radial variation of wood anatomy in açoita-cavalo (Luehea divaricata and its influence on the specific gravity.

    Directory of Open Access Journals (Sweden)

    Eduardo Luiz LONGUI

    2009-12-01

    Full Text Available Variações entre as dimensões e frequênciasdas células da madeira existem entre espéciesdiferentes, entre indivíduos da mesma espécie ouainda dentro da mesma árvore nas direções radial eaxial. Essa variação influencia diretamente nadensidade aparente e consequentemente no uso dasdiferentes madeiras. O presente estudo determinoua variação anatômica radial e sua influência nadensidade aparente no lenho de árvores de Luehea divaricata, a partir de amostras coletadas no DAP,por meio de método destrutivo. Para a avaliaçãoanatômica e da densidade aparente foram empregadastécnicas usuais em anatomia e propriedades físicasda madeira e os dados tratados estatisticamente.Os resultados revelaram que o diâmetro dos vasos,espessura da parede das fibras e a densidadeaparente foram significativamente menores na regiãoda medula, já o lume das fibras foi significativamentemaior nesta região, que também apresentou raiosmais volumosos. Sugere-se que fibras com paredesmais finas e raios com maior volume na região damedula contribuíram de forma determinante paraos menores valores de densidade aparente.Variations between the size and frequencyof the wood cells occur among differentspecies, trees of the same species or even withinthe same tree in the radial and axial directions.These variations affect directly the specific gravityand consequently the use of different woods.This study determined the radial anatomicalvariation and its influence on specific gravity ofthe wood of Luehea divaricata trees, from samplescollected on DBH by using destructive method.For the evaluation of anatomical characteristicsand specific gravity were employed standardtechniques in anatomy and physical propertiesof wood and the data analyzed statistically.The results revealed significantly lower vesseldiameter, fiber wall thickness and specific gravityin pith, fiber lumen was significantly higher inthis region, which also showed very large rays.It is suggested

  9. Gis-approach for variability assessment of soil electric conductivity under pedoturbation activity of mole rat (Spalax microphthalmus

    Directory of Open Access Journals (Sweden)

    T. М. Konovalova

    2010-06-01

    Full Text Available The results of the investigation of the impact of the mole rat’s activity on soil electric conductivity have been presented. GIS-technology have been shown to be effective for assessment of the pedoturbation activity effect on the soil surface heterogeneity formation. Method of the one-dimension spatial coordinated array transformation into matrix form has been proposed for following multidimension statistic analysis application. The quantity estimation of the mole rats role in formation of the habitat nanorelief-level diversity has been obtained by means of indexes of the landscape complexity and diversity.

  10. Prevalence and Factors Associated with Hydatidiform Mole among Patients Undergoing Uterine Evacuation at Mbarara Regional Referral Hospital

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    Olivier Mulisya

    2018-01-01

    Full Text Available Objective. We sought to determine the prevalence of and factors associated with hydatidiform molar gestations amongst patients undergoing uterine evacuation at Mbarara Regional Referral Hospital (MRRH, Mbarara, Uganda. Methods. This was a cross-sectional study carried out from November 2016 to February 2017. All patients admitted for uterine evacuation for nonviable pregnancy were included. The study registered 181 patients. Data were collected on sociodemographics, medical conditions, obstetrics, and gynecological factors. The evacuated tissue received a full gross and histopathologic examination. Cases of pathologically suspected complete hydatidiform mole were confirmed by p57 immunohistochemistry. Data were analyzed using STATA 13. Results. The prevalence of hydatidiform mole was 6.1% (11/181. All detected moles were complete hydatidiform moles, and there were no diagnosed partial hydatidiform moles. Clinical diagnosis of molar pregnancy was suspected in 13 patients, but only 69.2% (9/13 were confirmed as molar pregnancies histologically. Two cases were clinically unsuspected. Factors that had a significant relationship with complete hydatidiform mole included maternal age of 35 years and above (aOR 13.5; CI: 1.46–125.31; p=0.00, gestational age beyond the first trimester at the time of uterine evacuation (aOR 6.2; CI: 1.07–36.14; p=0.04, and history of previous abortion (aOR 4.3; CI: 1.00–18.57; p=0.05. Conclusion. The prevalence of complete hydatidiform mole was high at 6.1%. Associated risk factors included advanced maternal age (35 years and above, history of previous abortions, and gestational age beyond the first trimester at the time of evacuations. Recommendations. We recommend putting in place capacity to do routine histopathological examination of all products of conception especially those at high risk for a molar gestation either by clinical suspicion or by risk factors including advanced maternal age, advanced gestational

  11. Spatial variability of soil electrical conductivity under the mole rats (Spalax microphthalmus digging activity at the different scales

    Directory of Open Access Journals (Sweden)

    A. V. Zhukov

    2012-07-01

    Full Text Available The soil mounds emerged owing to the mole rats’ digging activity have been shown to be characterised by less electrical conductivity than surrounded soil. This effect is due to the changes of the mounds bulk’s density and moisture. The effect of the mole rats’ digging activity on the soil electrical conductivity has been found not to be restricted by the geometrical border of the mounds. The mounds are surrounded by 1–1.5 m halo of increased soil electrical conductivity. The halo size is increased with the aging of the mound and with the compacting of their aggregation.

  12. Fingerprinting: Modelling and mapping physical top soil properties with the Mole

    Science.gov (United States)

    Loonstra, Eddie; van Egmond, Fenny

    2010-05-01

    The Mole is a passive gamma ray soil sensor system. It is designed for the mobile collection of radioactive energy stemming from soil. As the system is passive, it only measures energy that reaches the surface of soil. In general, this energy comes from upto 30 to 40 cm deep, which can be considered topsoil. The gathered energy spectra are logged every second, are processed with the method of Full Spectrum Analysis. This method uses all available spectral data and processes it with a Chi square optimalisation using a set of standard spectra into individual nuclide point data. A standard spectrum is the measured full spectrum of a specific detector derived when exposed to 1 Bq/kg of a nuclide. With this method the outcome of the surveys become quantitative.The outcome of a field survey with the Mole results in a data file containing point information of position, Total Counts and the decay products of 232Th, 238U, 40K and 137Cs. Five elements are therefor available for the modelling of soil properties. There are several ways for the modelling of soil properties with sensor derived gamma ray data. The Mole generates ratio scale output. For modelling a quantitative deterministic approach is used based on sample locations. This process is called fingerprinting. Fingerprinting is a comparison of the concentration of the radioactive trace elements and the lab results (pH, clay content, etc.) by regression analysis. This results in a mathematical formula describing the relationship between a dependent and independent property. The results of the sensor readings are interpolated into a nuclide map with GIS software. With the derived formula a soil property map is composed. The principle of fingerprinting can be applied on large geographical areas for physical soil properties such as clay, loam or sand (50 micron), grain size and organic matter. Collected sample data of previous field surveys within the same region can be used for the prediction of soil properties elsewhere

  13. Slip casting of thoria-10 mole per cent yttria solid electrolyte

    International Nuclear Information System (INIS)

    Ramanathan, S.; Rao, S.V.K.

    1990-01-01

    One end closed thoria-yttria solid electrolyte have been fabricated by the slip casting technique. A systematic study of the influence of the process parameters on the characteristics of the final bodies has been carried out. Slips of ThO 2 -10 mole % Y 2 O 3 were prepared; their fluidity and castability were studied as a function of concentration, pH and particle size. The bodies were sintered at 2000degC and the physical properties like density and microstructure were evaluated. Slip cast bodies of bulk densities around 95% T.D. with relatively inhomogenous but predominantly fine grained structure could be obtained by optimizing the process variables. (author). 5 figs., 10 refs

  14. Comparative analysis of genome maintenance genes in naked mole rat, mouse, and human.

    Science.gov (United States)

    MacRae, Sheila L; Zhang, Quanwei; Lemetre, Christophe; Seim, Inge; Calder, Robert B; Hoeijmakers, Jan; Suh, Yousin; Gladyshev, Vadim N; Seluanov, Andrei; Gorbunova, Vera; Vijg, Jan; Zhang, Zhengdong D

    2015-04-01

    Genome maintenance (GM) is an essential defense system against aging and cancer, as both are characterized by increased genome instability. Here, we compared the copy number variation and mutation rate of 518 GM-associated genes in the naked mole rat (NMR), mouse, and human genomes. GM genes appeared to be strongly conserved, with copy number variation in only four genes. Interestingly, we found NMR to have a higher copy number of CEBPG, a regulator of DNA repair, and TINF2, a protector of telomere integrity. NMR, as well as human, was also found to have a lower rate of germline nucleotide substitution than the mouse. Together, the data suggest that the long-lived NMR, as well as human, has more robust GM than mouse and identifies new targets for the analysis of the exceptional longevity of the NMR. © 2015 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

  15. The effect of carbon mole ratio on the fabrication of silicon carbide

    Directory of Open Access Journals (Sweden)

    Sutham Niyomwas

    2008-03-01

    Full Text Available Silicon Carbide (SiC particles were synthesized by self-propagating high temperature synthesis (SHS from a powder mixture of SiO2-C-Mg. The reaction was carried out in a SHS reactor under static argon gas at a pressure of 0.5 MPa. The standard Gibbs energy minimization method was used to calculate the equilibrium composition of the reacting species. The effects of carbon mole ratio on the precursor mixture (C/SiO2/Mg: 1/1/2 to 3/1/2 and on the SiC conversion were investigated using X-ray diffraction and scanning electron microscope technique. The as-synthesized products of SiC-MgO powders were leached with 0.1M HCl acid solution to obtain the SiC particles.

  16. Ethnotaxonomical Study of Mole Crab (Crustacea:Hippoidea on Coastal Community of Cilacap

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    Dian Bhagawati

    2016-09-01

    Full Text Available Local wisdom is a cultural heritage that needs to be preserved. The Cilacap’s coastal communities have traditional taxonomy knowledge that hasn’t been informed, especially about mole crab. This study aimed to explore the ethnotaxonomy knowledge of sand crabs (Crustacea: Hippoidea in Widarapayung coastal communities. The purposive snowball sampling was used for the method, followed by observation and semi-structured interviews with 30 respondents. Data were analyzed descriptively based on observations and interviews. The results showed that the coastal communities in Widarapayung tourist areas could be divided into five groups, namely catchers, collectors, processors, traders, and consumers. The catcher was able to recognize and describe three types of sand crabs based on the shape of the body and their catchment areas. Captured crabs had the local name of ‘yutuk jambe’ (Emerita emeritus Linnaeus 1767, the appellation was based on carapace shape that resembled the shape of areca fruit; ‘Yutuk bathok’ (Hippa adactyla Fabricius 1787, had the carapace shape resembling a coconut shell, and ‘yutuk Kethek’ (Albunea symmysta Linnaeus 1758, had ornaments on the carapace surface similar with a monkey face, there were dense setae on the edge of the carapace, spiny long antennas and aggressively pinch when captured. The knowledge and skills of sand crabs classification and local name entitlement acquired by the people were in accordance with the Berlin’s model. How to CiteBhagawati, D., Anggoro, S., Zainuri, M. & Sya’rani, L. (2016. Ethnotaxonomical Study of Mole Crab (Crustacea:Hippoidea on Coastal Community of Cilacap. Biosaintifika: Journal of Biology & Biology Education, 8(2, 222-230.

  17. High accuracy measurements of dry mole fractions of carbon dioxide and methane in humid air

    Science.gov (United States)

    Rella, C. W.; Chen, H.; Andrews, A. E.; Filges, A.; Gerbig, C.; Hatakka, J.; Karion, A.; Miles, N. L.; Richardson, S. J.; Steinbacher, M.; Sweeney, C.; Wastine, B.; Zellweger, C.

    2013-03-01

    Traditional techniques for measuring the mole fractions of greenhouse gases in the well-mixed atmosphere have required dry sample gas streams (dew point < -25 °C) to achieve the inter-laboratory compatibility goals set forth by the Global Atmosphere Watch programme of the World Meteorological Organisation (WMO/GAW) for carbon dioxide (±0.1 ppm in the Northern Hemisphere and ±0.05 ppm in the Southern Hemisphere) and methane (±2 ppb). Drying the sample gas to low levels of water vapour can be expensive, time-consuming, and/or problematic, especially at remote sites where access is difficult. Recent advances in optical measurement techniques, in particular cavity ring down spectroscopy, have led to the development of greenhouse gas analysers capable of simultaneous measurements of carbon dioxide, methane and water vapour. Unlike many older technologies, which can suffer from significant uncorrected interference from water vapour, these instruments permit accurate and precise greenhouse gas measurements that can meet the WMO/GAW inter-laboratory compatibility goals (WMO, 2011a) without drying the sample gas. In this paper, we present laboratory methodology for empirically deriving the water vapour correction factors, and we summarise a series of in-situ validation experiments comparing the measurements in humid gas streams to well-characterised dry-gas measurements. By using the manufacturer-supplied correction factors, the dry-mole fraction measurements have been demonstrated to be well within the GAW compatibility goals up to a water vapour concentration of at least 1%. By determining the correction factors for individual instruments once at the start of life, this water vapour concentration range can be extended to at least 2% over the life of the instrument, and if the correction factors are determined periodically over time, the evidence suggests that this range can be extended up to and even above 4% water vapour concentrations.

  18. Molecular basis of a novel adaptation to hypoxic-hypercapnia in a strictly fossorial mole

    Directory of Open Access Journals (Sweden)

    Bonaventura Joseph

    2010-07-01

    Full Text Available Abstract Background Elevated blood O2 affinity enhances survival at low O2 pressures, and is perhaps the best known and most broadly accepted evolutionary adjustment of terrestrial vertebrates to environmental hypoxia. This phenotype arises by increasing the intrinsic O2 affinity of the hemoglobin (Hb molecule, by decreasing the intracellular concentration of allosteric effectors (e.g., 2,3-diphosphoglycerate; DPG, or by suppressing the sensitivity of Hb to these physiological cofactors. Results Here we report that strictly fossorial eastern moles (Scalopus aquaticus have evolved a low O2 affinity, DPG-insensitive Hb - contrary to expectations for a mammalian species that is adapted to the chronic hypoxia and hypercapnia of subterranean burrow systems. Molecular modelling indicates that this functional shift is principally attributable to a single charge altering amino acid substitution in the β-type δ-globin chain (δ136Gly→Glu of this species that perturbs electrostatic interactions between the dimer subunits via formation of an intra-chain salt-bridge with δ82Lys. However, this replacement also abolishes key binding sites for the red blood cell effectors Cl-, lactate and DPG (the latter of which is virtually absent from the red cells of this species at δ82Lys, thereby markedly reducing competition for carbamate formation (CO2 binding at the δ-chain N-termini. Conclusions We propose this Hb phenotype illustrates a novel mechanism for adaptively elevating the CO2 carrying capacity of eastern mole blood during burst tunnelling activities associated with subterranean habitation.

  19. Adaptation of pelage color and pigment variations in Israeli subterranean blind mole rats, Spalax ehrenbergi [corrected].

    Directory of Open Access Journals (Sweden)

    Natarajan Singaravelan

    Full Text Available Concealing coloration in rodents is well established. However, only a few studies examined how soil color, pelage color, hair-melanin content, and genetics (i.e., the causal chain synergize to configure it. This study investigates the causal chain of dorsal coloration in Israeli subterranean blind mole rats, Spalax ehrenbergi.We examined pelage coloration of 128 adult animals from 11 populations belonging to four species of Spalax ehrenbergi superspecies (Spalax galili, Spalax golani, Spalax carmeli, and Spalax judaei and the corresponding coloration of soil samples from the collection sites using a digital colorimeter. Additionally, we quantified hair-melanin contents of 67 animals using HPLC and sequenced the MC1R gene in 68 individuals from all four mole rat species.Due to high variability of soil colors, the correlation between soil and pelage color coordinates was weak and significant only between soil hue and pelage lightness. Multiple stepwise forward regression revealed that soil lightness was significantly associated with all pelage color variables. Pelage color lightness among the four species increased with the higher southward aridity in accordance to Gloger's rule (darker in humid habitats and lighter in arid habitats. Darker and lighter pelage colors are associated with darker basalt and terra rossa, and lighter rendzina soils, respectively. Despite soil lightness varying significantly, pelage lightness and eumelanin converged among populations living in similar soil types. Partial sequencing of the MC1R gene identified three allelic variants, two of which were predominant in northern species (S. galili and S. golani, and the third was exclusive to southern species (S. carmeli and S. judaei, which might have caused the differences found in pheomelanin/eumelanin ratio.Darker dorsal pelage in darker basalt and terra rossa soils in the north and lighter pelage in rendzina and loess soils in the south reflect the combined results of

  20. Ear Structures of the Naked Mole-Rat, Heterocephalus glaber, and Its Relatives (Rodentia: Bathyergidae.

    Directory of Open Access Journals (Sweden)

    Matthew J Mason

    Full Text Available Although increasingly popular as a laboratory species, very little is known about the peripheral auditory system of the naked mole-rat, Heterocephalus glaber. In this study, middle and inner ears of naked mole-rats of a range of ages were examined using micro-computed tomography and dissection. The ears of five other bathyergid species (Bathyergus suillus, Cryptomys hottentotus, Fukomys micklemi, Georychus capensis and Heliophobius argenteocinereus were examined for comparative purposes. The middle ears of bathyergids show features commonly found in other members of the Ctenohystrica rodent clade, including a fused malleus and incus, a synovial stapedio-vestibular articulation and the loss of the stapedius muscle. Heterocephalus deviates morphologically from the other bathyergids examined in that it has a more complex mastoid cavity structure, poorly-ossified processes of the malleus and incus, a 'columelliform' stapes and fewer cochlear turns. Bathyergids have semicircular canals with unusually wide diameters relative to their radii of curvature. How the lateral semicircular canal reaches the vestibule differs between species. Heterocephalus has much more limited high-frequency hearing than would be predicted from its small ear structures. The spongy bone forming its ossicular processes, the weak incudo-stapedial articulation, the columelliform stapes and (compared to other bathyergids reduced cochlear coiling are all potentially degenerate features which might reflect a lack of selective pressure on its peripheral auditory system. Substantial intraspecific differences were found in certain middle and inner ear structures, which might also result from relaxed selective pressures. However, such interpretations must be treated with caution in the absence of experimental evidence.

  1. Social condition and oxytocin neuron number in the hypothalamus of naked mole-rats (Heterocephalus glaber).

    Science.gov (United States)

    Mooney, S J; Holmes, M M

    2013-01-29

    The naked mole-rat is a subterranean colonial rodent. In each colony, which can grow to as many as 300 individuals, there is only one female and 1-3 males that are reproductive and socially dominant. The remaining animals are reproductively suppressed subordinates that contribute to colony survival through their cooperative behaviors. Oxytocin is a peptide hormone that has shown relatively widespread effects on prosocial behaviors in other species. We examined whether social status affects the number of oxytocin-immunoreactive neurons in the paraventricular nucleus and the supraoptic nucleus by comparing dominant breeding animals to subordinate non-breeding workers from intact colonies. We also examined these regions in subordinate animals that had been removed from their colony and paired with an opposite- or same-sex conspecific for 6 months. Stereological analyses indicated that subordinates had significantly more oxytocin neurons in the paraventricular nucleus than breeders. Animals in both opposite- and same-sex pairs showed a decreased oxytocin neuron number compared to subordinates suggesting that status differences may be due to social condition rather than the reproductive activity of the animal per se. The effects of social status appear to be region specific as no group differences were found for oxytocin neuron number in the supraoptic nucleus. Given that subordinate naked mole-rats are kept reproductively suppressed through antagonism by the queen, we speculate that status differences are due either to oxytocin's anxiolytic properties to combat the stress of this antagonism or to its ability to promote the prosocial behaviors of subordinates. Copyright © 2012 IBRO. Published by Elsevier Ltd. All rights reserved.

  2. Ear Structures of the Naked Mole-Rat, Heterocephalus glaber, and Its Relatives (Rodentia: Bathyergidae).

    Science.gov (United States)

    Mason, Matthew J; Cornwall, Hannah L; Smith, Ewan St J

    2016-01-01

    Although increasingly popular as a laboratory species, very little is known about the peripheral auditory system of the naked mole-rat, Heterocephalus glaber. In this study, middle and inner ears of naked mole-rats of a range of ages were examined using micro-computed tomography and dissection. The ears of five other bathyergid species (Bathyergus suillus, Cryptomys hottentotus, Fukomys micklemi, Georychus capensis and Heliophobius argenteocinereus) were examined for comparative purposes. The middle ears of bathyergids show features commonly found in other members of the Ctenohystrica rodent clade, including a fused malleus and incus, a synovial stapedio-vestibular articulation and the loss of the stapedius muscle. Heterocephalus deviates morphologically from the other bathyergids examined in that it has a more complex mastoid cavity structure, poorly-ossified processes of the malleus and incus, a 'columelliform' stapes and fewer cochlear turns. Bathyergids have semicircular canals with unusually wide diameters relative to their radii of curvature. How the lateral semicircular canal reaches the vestibule differs between species. Heterocephalus has much more limited high-frequency hearing than would be predicted from its small ear structures. The spongy bone forming its ossicular processes, the weak incudo-stapedial articulation, the columelliform stapes and (compared to other bathyergids) reduced cochlear coiling are all potentially degenerate features which might reflect a lack of selective pressure on its peripheral auditory system. Substantial intraspecific differences were found in certain middle and inner ear structures, which might also result from relaxed selective pressures. However, such interpretations must be treated with caution in the absence of experimental evidence.

  3. Peripheral administration of oxytocin increases social affiliation in the naked mole-rat (Heterocephalus glaber).

    Science.gov (United States)

    Mooney, Skyler J; Douglas, Natasha R; Holmes, Melissa M

    2014-04-01

    The neuropeptide oxytocin regulates a wide variety of social behaviors across diverse species. However, the types of behaviors that are influenced by this hormone are constrained by the species in question and the social organization that a particular species exhibits. Therefore, the present experiments investigated behaviors regulated by oxytocin in a eusocial mammalian species by using the naked mole-rat (Heterocephalus glaber). In Experiment 1, adult non-breeding mole-rats were given intraperitoneal injections of either oxytocin (1mg/kg or 10mg/kg) or saline on alternate days. Animals were then returned to their colony and behavior was recorded for minutes 15-30 post-injection. Both doses of oxytocin increased huddling behavior during this time period. In Experiment 2, animals received intraperitoneal injections of either oxytocin (1mg/kg), an oxytocin-receptor antagonist (0.1mg/kg), a cocktail of oxytocin and the antagonist, or saline across 4 testing days in a counterbalanced design. Animals were placed in either a 2-chamber arena with a familiar conspecific or in a small chamber with 1week old pups from their home colony and behaviors were recorded for minutes 15-30 post-injection. Oxytocin increased investigation of, and time spent in close proximity to, a familiar conspecific; these effects were blocked by the oxytocin antagonist. No effects were seen on pup-directed behavior. These data suggest that oxytocin is capable of modulating affiliative-like behavior in this eusocial species. Copyright © 2014 Elsevier Inc. All rights reserved.

  4. Effect of Temperature and Mole Ratio on the Synthesis Yield of Rhenium-Tetrofosmin

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    Widyastuti

    2015-08-01

    Full Text Available Technetium-99m (99mTc tetrofosmin is widely used in nuclear medicine as a diagnostic agent for myocardial perfusion and as a tumor imaging agent. As a parenteral preparation it requires an evaluation of its pharmacokinetics and stability in-vivo. Since 99mTc has a short half-life and is only available in very low concentrations, it is impossible to characterize its chemical properties and presence in the body. Due to this reason, only technetium-99 (T1/2 = 5 × 105 years, which is available in macro quantities, or natural rhenium can be used for this purpose. In this study rhenium-188 (188Re tetrofosmin will be synthesized and applied, because non-radioactive Re can be easily obtained. Synthesis and radiochemical purity analysis of carrier-added 188Re-tetrofosmin were carried out as a model to study the in-vivo stability of technetium-99m tetrofosmin. Rhenium-188 was used as a tracer to identify the formation of rhenium tetrofosmin. Rhenium gluconate was synthesized first prior to the formation of rhenium tetrofosmin. The quality of labeling for both rhenium gluconate and rhenium tetrofosmin was analyzed using paper- and thin-layer chromatography, respectively. Rhenium gluconate can be synthesized with high labeling yield within 1 hour, whereas rhenium tetrofosmin was synthesized both in room temperature and in an elevated temperature with various tetrofosmin-to-rhenium mole ratios.The results showed that heating at 95oC led to a higher yield of more than 90% within 30 minutes. Rhenium tetrofosmin could be produced in high radiochemical purity using an excess of tetrofosmin with mole ratio of 2000. It is concluded that rhenium tetrofosmin could be synthesized through the formation of rhenium gluconate, and a higher yield could be obtained in a shorter time by heating process.

  5. Efeito da data de sementeira na produtividade de trigo mole (Triticum Aestivum L. em condições mediterrânicas Effect of the sowing date on bread wheat (Triticum Aestivum L. productivity under mediterranean conditions

    Directory of Open Access Journals (Sweden)

    José Manuel Godinho Calado

    2008-01-01

    Full Text Available A data de sementeira do trigo mole exerce uma influência relevante no crescimento e desenvolvimento da cultura, condicionando a sua produtividade. O seu efeito é, especial-mente, importante sob condições mediterrânicas. Por isso, neste trabalho procurou-se verificar a influência da data de sementeira no rendimento do trigo mole, a partir de um ensaio de quinze genótipos semeados em datas diferentes durante cinco anos (1994/95 a 1999/00 na região Alentejo. Com base na análise dos resultados conclui-se que a variação da época de sementeira em aproximadamente um mês, desde o fim de Outubro até ao fim de Novembro, pode permitir rendimentos viáveis do trigo mole nas zonas cerealíferas mediterrânicas, particularmente na região Alentejo. Todavia, as produções mais altas tendem a ser obtidas quando a sementeira é efectuada na primeira quinzena de Novembro. Ao avançar a sementeira para o mês de Dezembro decresce a produtividade e, em consequência, diminui a viabilidade da cultura.The sowing date of bread wheat has a great influence on the growth and development of the crop and its productivity, especially under Mediterranean conditions. This paper presents the results of trials conducted in the Alentejo region that studied the performance of fifteen wheat genotypes sown at different dates, repeated over 5 years (1994/95 to 1999/00. Despite a great variability between years, the results indicate that a satisfactory wheat productivity in the Alentejo can be obtained with sowing dates ranging from end of October until the end of November. Nonetheless, sowing dates in the first half of November tend to provide higher grain yields. Moving the sowing date into December results in reduced grain yields, thus compromising the economic viability of wheat growing.

  6. Undifferentiated carcinoma with osteoclastic giant cells (UCOCGC) of the pancreas associated with the familial atypical multiple mole melanoma syndrome (FAMMM)

    NARCIS (Netherlands)

    Koorstra, Jan-Bart M.; Maitra, Anirban; Morsink, Folkert H. M.; Drillenburg, Paul; ten Kate, Fiebo J. W.; Hruban, Ralph H.; Offerhaus, Johan A.

    2008-01-01

    The familial atypical multiple mole melanoma (FAMMM) syndrome is caused by a germline mutation of p16. More than 90% of the sporadic pancreatic carcinomas contain genetic alterations that inactivate p16. Patients with the FAMMM syndrome have an increased risk of developing pancreatic cancer. Ductal

  7. Development of Two-Tier Diagnostic Test Pictorial-Based for Identifying High School Students Misconceptions on the Mole Concept

    Science.gov (United States)

    Siswaningsih, W.; Firman, H.; Zackiyah; Khoirunnisa, A.

    2017-02-01

    The aim of this study was to develop the two-tier pictorial-based diagnostic test for identifying student misconceptions on mole concept. The method of this study is used development and validation. The development of the test Obtained through four phases, development of any items, validation, determination key, and application test. Test was developed in the form of pictorial consisting of two tier, the first tier Consist of four possible answers and the second tier Consist of four possible reasons. Based on the results of content validity of 20 items using the CVR (Content Validity Ratio), a number of 18 items declared valid. Based on the results of the reliability test using SPSS, Obtained 17 items with Cronbach’s Alpha value of 0703, the which means that items have accepted. A total of 10 items was conducted to 35 students of senior high school students who have studied the mole concept on one of the high schools in Cimahi. Based on the results of the application test, student misconceptions were identified in each label concept in mole concept with the percentage of misconceptions on the label concept of mole (60.15%), Avogadro’s number (34.28%), relative atomic mass (62, 84%), relative molecule mass (77.08%), molar mass (68.53%), molar volume of gas (57.11%), molarity (71.32%), chemical equation (82.77%), limiting reactants (91.40%), and molecular formula (77.13%).

  8. Vocalisations of the silvery mole-rat: comparasion of vocal repertoires in subterrane an rodents with different socials systems

    Czech Academy of Sciences Publication Activity Database

    Knotková, E.; Veitl, S.; Šumbera, R.; Sedláček, František; Burda, H.

    2009-01-01

    Roč. 18, č. 3 (2009), s. 241-257 ISSN 0952-4622 Institutional research plan: CEZ:AV0Z60870520 Keywords : silvery mole-rat * bathyergidae * vocal communication Subject RIV: EG - Zoology Impact factor: 1.050, year: 2009

  9. Functional neurokinin and NMDA receptor activity in an animal naturally lacking substance P: the naked mole-rat.

    Directory of Open Access Journals (Sweden)

    Antje Brand

    Full Text Available Naked mole-rats are extremely unusual among mammals in that their cutaneous C-fibers lack the neuropeptide Substance P (SP. In other mammals, SP plays an important role in nociception: it is released from C-fibers onto spinal neurons where it facilitates NMDA receptor activity and causes sensitization that can last for minutes, hours or days. In the present study, we tested the effects of intrathecal application of: 1 SP, 2 an SP antagonist (GR-82334, and 3 an NMDA antagonist (APV on heat-evoked foot withdrawal. In the naked mole-rat, at a high enough concentration, application of SP caused a large, immediate, and long-lasting sensitization of foot withdrawal latency that was transiently reversed by application of either antagonist. However, neither SP nor NMDA antagonists had an effect when administered alone to naïve animals. In contrast, both antagonists induced an increase in basal withdrawal latency in mice. These results indicate that spinal neurons in naked mole-rats have functional SP and NMDA receptors, but that these receptors do not participate in heat-evoked foot withdrawal unless SP is experimentally introduced. We propose that the natural lack of SP in naked mole-rat C-fibers may have resulted during adaptation to living in a chronically high carbon dioxide, high ammonia environment that, in other mammals, would stimulate C-fibers and evoke nocifensive behavior.

  10. Four Cases of Spontaneous Neoplasia in the Naked Mole-Rat (Heterocephalus glaber), A Putative Cancer-Resistant Species.

    Science.gov (United States)

    Taylor, Kyle R; Milone, Nicholas A; Rodriguez, Carlos E

    2017-01-01

    The naked mole-rat (Heterocephalus glaber) is widely acclaimed to be cancer-resistant and of considerable research interest based on a paucity of reports of neoplasia in this species. We have, however, encountered four spontaneous cases of neoplasia and one presumptive case of neoplasia through routine necropsy and biopsy of individuals in a zoo collection of nonhybrid naked mole-rats bred from a single pair. One case each of metastasizing hepatocellular carcinoma, nephroblastoma (Wilms' tumor), and multicentric lymphosarcoma, as well as presumptive esophageal adenocarcinoma (Barrett's esophagus-like) was identified postmortem among 37 nonautolyzed necropsy submissions of naked mole-rats over 1-year-old that were submitted for necropsy between 1998 and August 2015. One incidental case of cutaneous hemangioma was also identified antemortem by skin biopsy from one naked mole-rat examined for trauma. © The Author 2016. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  11. A cytosolic protein factor from the naked mole-rat activates proteasomes of other species and protects these from inhibition

    Science.gov (United States)

    Rodriguez, Karl A.; Osmulski, Pawel A.; Pierce, Anson; Weintraub, Susan T.; Gaczynska, Maria; Buffenstein, Rochelle

    2015-01-01

    The naked mole-rat maintains robust proteostasis and high levels of proteasome-mediated proteolysis for most of its exceptional (~31y) life span. Here, we report that the highly active proteasome from the naked mole-rat liver resists attenuation by a diverse suite of proteasome-specific small molecule inhibitors. Moreover, mouse, human, and yeast proteasomes exposed to the proteasome-depleted, naked mole-rat cytosolic fractions, recapitulate the observed inhibition resistance, and mammalian proteasomes also show increased activity. Gel filtration coupled with mass spectrometry and atomic force microscopy indicates that these traits are supported by a protein factor that resides in the cytosol. This factor interacts with the proteasome and modulates its activity. Although HSP72 and HSP40 (Hdj1) are among the constituents of this factor, the observed phenomenon, such as increasing peptidase activity and protecting against inhibition cannot be reconciled with any known chaperone functions. This novel function may contribute to the exceptional protein homeostasis in the naked mole-rat and allow it to successfully defy aging. PMID:25018089

  12. Functional neurokinin and NMDA receptor activity in an animal naturally lacking substance P: the naked mole-rat.

    Science.gov (United States)

    Brand, Antje; Smith, Ewan St J; Lewin, Gary R; Park, Thomas J

    2010-12-21

    Naked mole-rats are extremely unusual among mammals in that their cutaneous C-fibers lack the neuropeptide Substance P (SP). In other mammals, SP plays an important role in nociception: it is released from C-fibers onto spinal neurons where it facilitates NMDA receptor activity and causes sensitization that can last for minutes, hours or days. In the present study, we tested the effects of intrathecal application of: 1) SP, 2) an SP antagonist (GR-82334), and 3) an NMDA antagonist (APV) on heat-evoked foot withdrawal. In the naked mole-rat, at a high enough concentration, application of SP caused a large, immediate, and long-lasting sensitization of foot withdrawal latency that was transiently reversed by application of either antagonist. However, neither SP nor NMDA antagonists had an effect when administered alone to naïve animals. In contrast, both antagonists induced an increase in basal withdrawal latency in mice. These results indicate that spinal neurons in naked mole-rats have functional SP and NMDA receptors, but that these receptors do not participate in heat-evoked foot withdrawal unless SP is experimentally introduced. We propose that the natural lack of SP in naked mole-rat C-fibers may have resulted during adaptation to living in a chronically high carbon dioxide, high ammonia environment that, in other mammals, would stimulate C-fibers and evoke nocifensive behavior.

  13. Dynamic Thermodynamics with Internal Energy, Volume, and Amount of Moles as States : Application to Liquefied Gas Tank

    NARCIS (Netherlands)

    Arendsen, A. R. J.; Versteeg, G. F.

    2009-01-01

    Dynamic models for process design, optimization, and control usually solve a set of heat and/or mass balances as a function of time and/or position in the process. To obtain more robust dynamic models and to minimize the amount of assumptions, internal energy, volume, and amount of moles are chosen

  14. Photic induction of Fos in the suprachiasmatic nucleus of African mole-rats: responses to increasing irradiance.

    Science.gov (United States)

    Oosthuizen, Maria K; Bennett, Nigel C; Cooper, Howard M

    2010-09-01

    African mole-rats (family Bathyergidae) are strictly subterranean rodent species that are rarely exposed to environmental light. Morphological and physiological adaptations to the underground environment include a severely reduced eye size and regressed visual system. Responses of the circadian system to light, however, appear to be intact, since mole-rats are able to entrain their circadian activity rhythms to the light-dark cycle and light induces Fos expression in the suprachiasmatic nucleus (SCN). Social organization varies from solitary species to highly elaborated eusocial structures, characterized by a distinct division of labor and in which one reproductive female regulates the behavior and reproductive physiology of other individuals in the colony. The authors studied light-induced Fos expression in the SCN to increasing light intensities in four mole-rat species, ranging from strictly solitary to highly social. In the solitary Cape mole-rat, light induces significant Fos expression in the SCN, and the number of Fos-immunopositive cells increases with increasing light intensity. In contrast, Fos induction in the SCN of social species was slightly greater than, but not statistically different from, the dark-control animals as is typical of most rodents. One species showed a trend for an increase in expression with increased light, whereas a second species showed no trend in expression. In the naked mole-rat, Fos expression appeared higher in the dark-controls than in the animals exposed to light, although the differences in Fos expression were not significant. These results suggest a gradient in the sensitivity of the circadian system to light in mole-rats, with a higher percentage of individuals that are unresponsive to light in correlation with the degree of sociality. In highly social species, such as the naked mole-rat that live in a relatively stable subterranean milieu in terms of food availability, temperature, constant darkness, and devoid of 24-h

  15. The effects of oxotremorine, epibatidine, atropine, mecamylamine and naloxone in the tail-flick, hot-plate, and formalin tests in the naked mole-rat (Heterocephalus glaber)

    DEFF Research Database (Denmark)

    Dulu, Thomas D; Kanui, Titus I; Towett, Philemon K

    2014-01-01

    The naked mole-rat (Heterocephalus glaber) is a promising animal model for the study of pain mechanisms, therefore a thorough characterization of this species is essential. The aim of the present study was to establish the naked mole-rat as a model for studying the cholinergic receptor system in ...

  16. Expression of acid-sensing ion channels and selection of reference genes in mouse and naked mole rat.

    Science.gov (United States)

    Schuhmacher, Laura-Nadine; Smith, Ewan St John

    2016-12-13

    Acid-sensing ion channels (ASICs) are a family of ion channels comprised of six subunits encoded by four genes and they are expressed throughout the peripheral and central nervous systems. ASICs have been implicated in a wide range of physiological and pathophysiological processes: pain, breathing, synaptic plasticity and excitotoxicity. Unlike mice and humans, naked mole-rats do not perceive acid as a noxious stimulus, even though their sensory neurons express functional ASICs, likely an adaptation to living in a hypercapnic subterranean environment. Previous studies of ASIC expression in the mammalian nervous system have often not examined all subunits, or have failed to adequately quantify expression between tissues; to date there has been no attempt to determine ASIC expression in the central nervous system of the naked mole-rat. Here we perform a geNorm study to identify reliable housekeeping genes in both mouse and naked mole-rat and then use quantitative real-time PCR to estimate the relative amounts of ASIC transcripts in different tissues of both species. We identify RPL13A (ribosomal protein L13A) and CANX (calnexin), and β-ACTIN and EIF4A (eukaryotic initiation factor 4a) as being the most stably expressed housekeeping genes in mouse and naked mole-rat, respectively. In both species, ASIC3 was most highly expressed in dorsal root ganglia (DRG), and ASIC1a, ASIC2b and ASIC3 were more highly expressed across all brain regions compared to the other subunits. We also show that ASIC4, a proton-insensitive subunit of relatively unknown function, was highly expressed in all mouse tissues apart from DRG and hippocampus, but was by contrast the lowliest expressed ASIC in all naked mole-rat tissues.

  17. Assessing filtering of mountaintop CO2 mole fractions for application to inverse models of biosphere-atmosphere carbon exchange

    Directory of Open Access Journals (Sweden)

    S. L. Heck

    2012-02-01

    Full Text Available There is a widely recognized need to improve our understanding of biosphere-atmosphere carbon exchanges in areas of complex terrain including the United States Mountain West. CO2 fluxes over mountainous terrain are often difficult to measure due to unusual and complicated influences associated with atmospheric transport. Consequently, deriving regional fluxes in mountain regions with carbon cycle inversion of atmospheric CO2 mole fraction is sensitive to filtering of observations to those that can be represented at the transport model resolution. Using five years of CO2 mole fraction observations from the Regional Atmospheric Continuous CO2 Network in the Rocky Mountains (Rocky RACCOON, five statistical filters are used to investigate a range of approaches for identifying regionally representative CO2 mole fractions. Test results from three filters indicate that subsets based on short-term variance and local CO2 gradients across tower inlet heights retain nine-tenths of the total observations and are able to define representative diel variability and seasonal cycles even for difficult-to-model sites where the influence of local fluxes is much larger than regional mole fraction variations. Test results from two other filters that consider measurements from previous and following days using spline fitting or sliding windows are overly selective. Case study examples showed that these windowing-filters rejected measurements representing synoptic changes in CO2, which suggests that they are not well suited to filtering continental CO2 measurements. We present a novel CO2 lapse rate filter that uses CO2 differences between levels in the model atmosphere to select subsets of site measurements that are representative on model scales. Our new filtering techniques provide guidance for novel approaches to assimilating mountain-top CO2 mole fractions in carbon cycle inverse models.

  18. African Journals Online: Curaçao

    African Journals Online (AJOL)

    ... Cambodia, Cameroon, Canada, Cayman Islands, Central African Republic, Chad, Chile, China, Colombia, Comoros, Congo, Democratic Republic, Congo, Republic, Costa Rica, Côte d'Ivoire, Croatia, Cuba, Curaçao, Cyprus, Czech Republic, Denmark, Djibouti, Dominica, Dominican Republic, Ecuador, Egypt, Arab Rep.

  19. Nemerteans from Curaçao

    NARCIS (Netherlands)

    Corrêa, Diva Diniz

    1963-01-01

    All species treated in this paper were collected by me between January and June 1962, during my stay at the “Caraïbisch Marien-Biologisch Instituut” (Caribbean Marine Biological Institute), Curaçao. I am deeply indebted to the Government of the Netherlands, which awarded me a grant enabling me to

  20. SIG aplicado ao Ensino de Geografia

    OpenAIRE

    Dornelles, Liane Maria Azevedo; Universidade do Estado do Rio de Janeiro - UERJ

    2009-01-01

    Este trabalho descreve as atividades desenvolvidas junto à disciplina eletiva SIG aplicado ao ensino de Geografia. Foram elaboradas aplicações ambientais para os Ensinos Fundamental e Médio, com auxílio dos programas VistaSAGA/UFRJ, SISPLAMTE 5as com GIS, SPRING/INPE e MapServer.

  1. Turbellaria from Curaçao

    NARCIS (Netherlands)

    Marcus, Ernst

    1960-01-01

    A study of the several fresh and brackish-water springs on Curaçao, carried out by Dr. P. WAGENAAR HUMMELINCK, revealed (a) the common occurrence on that island of Dugesia festai (Borelli 1898), a species which until then had only been known from the South American mainland (nearest find: Ecuador!),

  2. Estados Unidos: do Iraque ao Brasil

    Directory of Open Access Journals (Sweden)

    Virgílio Caixeta Arraes

    2010-12-01

    Full Text Available O presente artigo busca analisar os recentes temas recorrentes na política externa norte-americana, caracterizada pela “guerra contra o terror”, e seus impactos para a agenda a ser discutida na vindoura visita do presidente dos Estados Unidos, Geoge W. Bush, ao Brasil.

  3. Uso de antagonistas e produtos alternativos no manejo pós-colheita de podridão mole em pimentão

    Directory of Open Access Journals (Sweden)

    Matheus Silva e Silva

    Full Text Available Visando o controle da podridão mole causada por Pectobacterium carotovorum subsp. carotovorum (Pcc em pimentão, foram avaliados 10 tratamentos: Bacillus spp.: isolado C116 (B. pumilus; Leveduras: isolados Lma (Rhodotorula aurantiaca e Lms (R. glutinis; Fosfito de potássio e cálcio; Fontes de cálcio: CaCl2, Ca(CO32 e Ca(SO42; fungicida/bactericida orgânico Antica®; antibiótico Kasumin®. Após a determinação da concentração dos antagonistas e produtos alternativos pelo teste do antibiograma, foi realizado o teste in vivo, pela inoculação do patógeno, seis horas após os tratamentos com os antagonistas e produtos alternativos. Foram determinados o Período de Incubação (PI, Severidade da doença (SEV, Redução da severidade da doença (RSD% e Área abaixo da curva de progresso da doença (AACPD. Todos os tratamentos testados apresentaram maiores valores de PI e RSD% e menores valores de SEV e AACPD quando comparados à testemunha. Fosfito de cálcio, C116, Lms, Lma e fosfito de potássio foram significativamente superiores quando comparados como demais tratamentos. Pcc foi resistente ao Kasumin®, possuindo SEV e AACPD que não diferiram significativamente da testemunha.

  4. Raman and luminescence spectroscopy of zirconium oxide with the use of the MOLE microprobe

    International Nuclear Information System (INIS)

    Doyle, T.E.; Alvarez, J.L.

    1984-01-01

    Raman and luminescence spectroscopy with the use of the MOLE microprobe has been used to characterize ZrO 2 originating from oxidized fuel-rod cladding in nuclear accidents. Micro-Raman analysis of samples from Three Mile Island Unit 2 and the Power Burst Facility identified tetragonal and cubic ZrO 2 . The tetragonal and cubic phases are high-temperature polymorphs of ZrO 2 and provide information about temperatures and hydrogen formation in the TMI-2 core. The data suggest that the tetragonal ZrO 2 in TMI-2 samples was stabilized by a crystallite size effect, whereas cubic ZrO 2 in PBF debris samples was stabilized by impurities. Luminescence was used to differentiate yttria-stabilized ZrO 2 ceramics and oxidized fuel-rod cladding in PBF debris samples. The ZrO 2 ceramics produced strong, sharp luminescence peaks which indicated the presence of titanium and yttria in the ZrO 2 . Oxidized fuel-rod cladding displayed no luminescence

  5. Influence of the mole penetrator on measurements of heat flow in lunar subsurface layers

    Science.gov (United States)

    Wawrzaszek, Roman; Drogosz, Michal; Seweryn, Karol; Banaszkiewicz, Marek; Grygorczuk, Jerzy

    Measuring the thermal gradient in subsurface layers is a basic method of determination the heat flux from the interior of a planetary body to its surface. In case of the Moon, such measurements complemented with the results of theoretical analysis and modeling can significantly improve our understanding of the thermal and geological evolution of the Moon. In practice, temperature gradient measurements are performed by at least two sensors located at different depths under the surface. These sensors will be attached to a penetrator [1] or to a cable pulled behind the penetrator. In both cases the object that carries the sensors, e.g. penetrator, perturb temperature measurements. In our study we analyze a case of two thermal sensors attached to the ends of 350mm long penetrator made of a composite material. In agreement with the studies of other authors we have found that the penetrator should be placed at the depth of 2-3 meters, where periodic changes of the temperature due to variation of solar flux at the surface are significantly smaller than the error of temperature measurement. The most important result of our analysis is to show how to deconvolve the real gradient of the temperature from the measurements perturbed by the penetrator body. In this way it will be possible to more accurately determine heat flux in the lunar regolith. [1] Grygorczuk J., Seweryn K., Wawrzaszek R., Banaszkiewicz M., Insertion of a Mole Pene-trator -Experimental Results, /39th Lunar and Planetary Science Conference /League City, Texas 2008

  6. Effectiveness of Using Computer-Assisted Supplementary Instruction for Teaching the Mole Concept

    Science.gov (United States)

    Yalçinalp, Serpil; Geban, Ömer; Özkan, Ilker

    This study examined the effect of computer-assisted instruction (CAI), used as a problem-solving supplement to classroom instruction, on students' understanding of chemical formulas and mole concept, their attitudes toward chemistry subjects, and CAI. The objective was to assess the effectiveness of CAI over recitation hours when both teaching methods were used as a supplement to the traditional chemistry instruction. We randomly selected two classes in a secondary school. Each teaching strategy was randomly assigned to one class. The experimental group received supplementary instruction delivered via CAI, while the control group received similar instruction through recitation hours. The data were analyzed using two-way analysis of variance and t-test. It was found that the students who used the CAI accompanied with lectures scored significantly higher than those who attended recitation hours, in terms of school subject achievement in chemistry and attitudes toward chemistry subjects. In addition, there was a significant improvement in the attitudes of students in the experimental group toward the use of computers in a chemistry course. There was no significant difference between the performances of females versus males in each treatment group.Received: 26 April 1994; Revised: 6 April 1995;

  7. Naked Mole Rat Cells Have a Stable Epigenome that Resists iPSC Reprogramming

    Directory of Open Access Journals (Sweden)

    Li Tan

    2017-11-01

    Full Text Available Naked mole rat (NMR is a valuable model for aging and cancer research due to its exceptional longevity and cancer resistance. We observed that the reprogramming efficiency of NMR fibroblasts in response to OSKM was drastically lower than that of mouse fibroblasts. Expression of SV40 LargeT antigen (LT dramatically improved reprogramming of NMR fibroblasts. Inactivation of Rb alone, but not p53, was sufficient to improve reprogramming efficiency, suggesting that NMR chromatin may be refractory to reprogramming. Analysis of the global histone landscape revealed that NMR had higher levels of repressive H3K27 methylation marks and lower levels of activating H3K27 acetylation marks than mouse. ATAC-seq revealed that in NMR, promoters of reprogramming genes were more closed than mouse promoters, while expression of LT led to massive opening of the NMR promoters. These results suggest that NMR displays a more stable epigenome that resists de-differentiation, contributing to the cancer resistance and longevity of this species.

  8. Renal Pathology in a Nontraditional Aging Model: The Naked Mole-Rat (Heterocephalus glaber).

    Science.gov (United States)

    Delaney, M A; Kinsel, M J; Treuting, P M

    2016-03-01

    The naked mole-rat (NMR; Heterocephalus glaber) is growing in popularity as a model for aging research due to its extreme longevity (up to 30 years), highly adapted physiology, and resistance to cancer, particularly when compared with traditional aging models such as laboratory mice and rats. Despite the NMR's seemingly lengthy health span, several age-related lesions have been documented. During a 15-year retrospective evaluation of a zoo-housed population, histologic changes in the kidneys were reported in 127 of 138 (92%) adult NMRs. Of these, renal tubular mineralization was very common (115 of 127; 90.6%) and found in NMRs without concurrent renal lesions (36 of 127; 28.3%). Many of the other described lesions were considered progressive stages of a single process, generally referred to as chronic nephritis or nephropathy, and diagnosed in 73 of 127 (57.5%), while end-stage renal disease was reported in only 12 (9.4%) NMRs. Renal lesions of these NMRs were comparable to disease entities reported in laboratory rats and certain strains of inbred and noninbred mice. Although many lesions of NMR kidneys were similar to those found in aged laboratory rodents, some common urinary diseases were not represented in the examined colonies. The goal of this study was to describe renal lesions in NMRs from a zoologic setting to familiarize investigators and pathologists with an apparently common and presumably age-related disease in this nontraditional model. © The Author(s) 2015.

  9. Naked Mole Rat Induced Pluripotent Stem Cells and Their Contribution to Interspecific Chimera

    Directory of Open Access Journals (Sweden)

    Sang-Goo Lee

    2017-11-01

    Full Text Available Naked mole rats (NMRs are exceptionally long-lived, cancer-resistant rodents. Identifying the defining characteristics of these traits may shed light on aging and cancer mechanisms. Here, we report the generation of induced pluripotent stem cells (iPSCs from NMR fibroblasts and their contribution to mouse-NMR chimeric embryos. Efficient reprogramming could be observed under N2B27+2i conditions. The iPSCs displayed a characteristic morphology, expressed pluripotent markers, formed embryoid bodies, and showed typical differentiation patterns. Interestingly, NMR embryonic fibroblasts and the derived iPSCs had propensity for a tetraploid karyotype and were resistant to forming teratomas, but within mouse blastocysts they contributed to both interspecific placenta and fetus. Gene expression patterns of NMR iPSCs were more similar to those of human than mouse iPSCs. Overall, we uncovered unique features of NMR iPSCs and report a mouse-NMR chimeric model. The iPSCs and associated cell culture systems can be used for a variety of biological and biomedical applications.

  10. Functional characteristics of the naked mole rat μ-opioid receptor.

    Directory of Open Access Journals (Sweden)

    Melanie Busch-Dienstfertig

    Full Text Available While humans and most animals respond to µ-opioid receptor (MOR agonists with analgesia and decreased aggression, in the naked mole rat (NMR opioids induce hyperalgesia and severe aggression. Single nucleotide polymorphisms in the human mu-opioid receptor gene (OPRM1 can underlie altered behavioral responses to opioids. Therefore, we hypothesized that the primary structure of the NMR MOR may differ from other species. Sequencing of the NMR oprm1 revealed strong homology to other mammals, but exposed three unique amino acids that might affect receptor-ligand interactions. The NMR and rat oprm1 sequences were cloned into mammalian expression vectors and transfected into HEK293 cells. Radioligand binding and 3'-5'-cyclic adenosine monophosphate (cAMP enzyme immunoassays were used to compare opioid binding and opioid-mediated cAMP inhibition. At normalized opioid receptor protein levels we detected significantly lower [3H]DAMGO binding to NMR compared to rat MOR, but no significant difference in DAMGO-induced cAMP inhibition. Strong DAMGO-induced MOR internalization was detectable using radioligand binding and confocal imaging in HEK293 cells expressing rat or NMR receptor, while morphine showed weak or no effects. In summary, we found minor functional differences between rat and NMR MOR suggesting that other differences e.g. in anatomical distribution of MOR underlie the NMR's extreme reaction to opioids.

  11. Cardiac function of the naked mole-rat: ecophysiological responses to working underground.

    Science.gov (United States)

    Grimes, Kelly M; Voorhees, Andrew; Chiao, Ying Ann; Han, Hai-Chao; Lindsey, Merry L; Buffenstein, Rochelle

    2014-03-01

    The naked mole-rat (NMR) is a strictly subterranean rodent with a low resting metabolic rate. Nevertheless, it can greatly increase its metabolic activity to meet the high energetic demands associated with digging through compacted soils in its xeric natural habitat where food is patchily distributed. We hypothesized that the NMR heart would naturally have low basal function and exhibit a large cardiac reserve, thereby mirroring the species' low basal metabolism and large metabolic scope. Echocardiography showed that young (2-4 yr old) healthy NMRs have low fractional shortening (28 ± 2%), ejection fraction (43 ± 2%), and cardiac output (6.5 ± 0.4 ml/min), indicating low basal cardiac function. Histology revealed large NMR cardiomyocyte cross-sectional area (216 ± 10 μm(2)) and cardiac collagen deposition of 2.2 ± 0.4%. Neither of these histomorphometric traits was considered pathological, since biaxial tensile testing showed no increase in passive ventricular stiffness. NMR cardiomyocyte fibers showed a low degree of rotation, contributing to the observed low NMR cardiac contractility. Interestingly, when the exercise mimetic dobutamine (3 μg/g ip) was administered, NMRs showed pronounced increases in fractional shortening, ejection fraction, cardiac output, and stroke volume, indicating an increased cardiac reserve. The relatively low basal cardiac function and enhanced cardiac reserve of NMRs are likely to be ecophysiological adaptations to life in an energetically taxing environment.

  12. Antagonistic Coevolution Drives Whack-a-Mole Sensitivity in Gene Regulatory Networks.

    Directory of Open Access Journals (Sweden)

    Jeewoen Shin

    2015-10-01

    Full Text Available Robustness, defined as tolerance to perturbations such as mutations and environmental fluctuations, is pervasive in biological systems. However, robustness often coexists with its counterpart, evolvability--the ability of perturbations to generate new phenotypes. Previous models of gene regulatory network evolution have shown that robustness evolves under stabilizing selection, but it is unclear how robustness and evolvability will emerge in common coevolutionary scenarios. We consider a two-species model of coevolution involving one host and one parasite population. By using two interacting species, key model parameters that determine the fitness landscapes become emergent properties of the model, avoiding the need to impose these parameters externally. In our study, parasites are modeled on species such as cuckoos where mimicry of the host phenotype confers high fitness to the parasite but lower fitness to the host. Here, frequent phenotype changes are favored as each population continually adapts to the other population. Sensitivity evolves at the network level such that point mutations can induce large phenotype changes. Crucially, the sensitive points of the network are broadly distributed throughout the network and continually relocate. Each time sensitive points in the network are mutated, new ones appear to take their place. We have therefore named this phenomenon "whack-a-mole" sensitivity, after a popular fun park game. We predict that this type of sensitivity will evolve under conditions of strong directional selection, an observation that helps interpret existing experimental evidence, for example, during the emergence of bacterial antibiotic resistance.

  13. Determination of mole fractions of ethyl-cellulose-containing monomers by NMR.

    Science.gov (United States)

    Kono, Hiroyuki

    2017-06-05

    Three samples of ethyl cellulose (EC) with different degrees of substitution (DS)-0.51, 1.41, and 2.28-were prepared by a slurry method using ethyl bromide as the etherification reagent. 1 H- 13 C HSQC and HSQC-TOCSY NMR spectral analysis allowed for complete assignment of the 1 H and 13 C chemical shifts, respectively, of eight anhydroglucose units (AGUs) comprising EC chains-un-, 2-mono-, 3-mono-, 6-mono-, 2,3-di-, 2,6-di-, 3,6-di-, and 2,3,6-tri-substituted AGUs. In addition, the lineshape of the quantitative 13 C NMR spectra of the three EC samples provided change in the mole fractions of these AGUs against DS, making it possible to estimate the reaction mechanism for the production of EC, elucidating reactivities of the hydroxyl groups at the 2, 3, and 6 positions of cellulose and interactions between the substituent groups within the same AGU and vicinal AGUs. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Molecular evidence for an association of a phytoplasma with apple rubbery wood

    OpenAIRE

    Ribeiro, Luiz Fernando Caldeira; Bedendo, Ivan Paulo; Sanhueza, Rosa Maria Valdebenito

    2007-01-01

    O lenho mole da macieira é uma doença relevante em diversas partes do mundo. Sintomas típicos desta doença têm sido observados em pomares instalados em estados do sul do território brasileiro desde a década de oitenta. Enxertia tem revelado a natureza infecciosa da doença e a observação de corpúsculos filamentosos no floema tem evidenciado possível associação com fitoplasma. No presente trabalho plantas com sintomas de lenho mole foram coletadas em pomar comercial, visando demonstrar a presen...

  15. RNA sequencing reveals differential expression of mitochondrial and oxidation reduction genes in the long-lived naked mole-rat when compared to mice.

    Science.gov (United States)

    Yu, Chuanfei; Li, Yang; Holmes, Andrew; Szafranski, Karol; Faulkes, Chris G; Coen, Clive W; Buffenstein, Rochelle; Platzer, Matthias; de Magalhães, João Pedro; Church, George M

    2011-01-01

    The naked mole-rat (Heterocephalus glaber) is a long-lived, cancer resistant rodent and there is a great interest in identifying the adaptations responsible for these and other of its unique traits. We employed RNA sequencing to compare liver gene expression profiles between naked mole-rats and wild-derived mice. Our results indicate that genes associated with oxidoreduction and mitochondria were expressed at higher relative levels in naked mole-rats. The largest effect is nearly 300-fold higher expression of epithelial cell adhesion molecule (Epcam), a tumour-associated protein. Also of interest are the protease inhibitor, alpha2-macroglobulin (A2m), and the mitochondrial complex II subunit Sdhc, both ageing-related genes found strongly over-expressed in the naked mole-rat. These results hint at possible candidates for specifying species differences in ageing and cancer, and in particular suggest complex alterations in mitochondrial and oxidation reduction pathways in the naked mole-rat. Our differential gene expression analysis obviated the need for a reference naked mole-rat genome by employing a combination of Illumina/Solexa and 454 platforms for transcriptome sequencing and assembling transcriptome contigs of the non-sequenced species. Overall, our work provides new research foci and methods for studying the naked mole-rat's fascinating characteristics.

  16. Adult naked mole-rat brain retains the NMDA receptor subunit GluN2D associated with hypoxia tolerance in neonatal mammals.

    Science.gov (United States)

    Peterson, Bethany L; Park, Thomas J; Larson, John

    2012-01-11

    Adult naked mole-rats show a number of systemic adaptations to a crowded underground habitat that is low in oxygen and high in carbon dioxide. Remarkably, brain slice tissue from adult naked mole-rats also is extremely tolerant to oxygen deprivation as indicated by maintenance of synaptic transmission under hypoxic conditions as well as by a delayed neuronal depolarization during anoxia. These characteristics resemble hypoxia tolerance in brain slices from neonates in a variety of mammal species. An important component of neonatal tolerance to hypoxia involves the subunit composition of NMDA receptors. Neonates have a high proportion of NMDA receptors with GluN2D subunits which are protective because they retard calcium entry into neurons during hypoxic episodes. Therefore, we hypothesized that adult naked mole-rats retain a protective, neonatal-like, NMDA receptor subunit profile. We used immunoblotting to assess age-related changes in NMDA receptor subunits in naked mole-rats and mice. The results show that adult naked mole-rat brain retains a much greater proportion of the hypoxia-protective GluN2D subunit compared to adult mice. However, age-related changes in other subunits (GluN2A and GluN2B) from the neonatal period to adulthood were comparable in mice and naked mole-rats. Hence, adult naked mole-rat brain only retains the neonatal NMDA receptor subunit that is associated with hypoxia tolerance. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  17. RNA sequencing reveals differential expression of mitochondrial and oxidation reduction genes in the long-lived naked mole-rat when compared to mice.

    Directory of Open Access Journals (Sweden)

    Chuanfei Yu

    Full Text Available The naked mole-rat (Heterocephalus glaber is a long-lived, cancer resistant rodent and there is a great interest in identifying the adaptations responsible for these and other of its unique traits. We employed RNA sequencing to compare liver gene expression profiles between naked mole-rats and wild-derived mice. Our results indicate that genes associated with oxidoreduction and mitochondria were expressed at higher relative levels in naked mole-rats. The largest effect is nearly 300-fold higher expression of epithelial cell adhesion molecule (Epcam, a tumour-associated protein. Also of interest are the protease inhibitor, alpha2-macroglobulin (A2m, and the mitochondrial complex II subunit Sdhc, both ageing-related genes found strongly over-expressed in the naked mole-rat. These results hint at possible candidates for specifying species differences in ageing and cancer, and in particular suggest complex alterations in mitochondrial and oxidation reduction pathways in the naked mole-rat. Our differential gene expression analysis obviated the need for a reference naked mole-rat genome by employing a combination of Illumina/Solexa and 454 platforms for transcriptome sequencing and assembling transcriptome contigs of the non-sequenced species. Overall, our work provides new research foci and methods for studying the naked mole-rat's fascinating characteristics.

  18. ENSAIO: HOMENAGEM AO PROFESSOR REINIER ROZESTRATEN

    Directory of Open Access Journals (Sweden)

    Antonio C. R. Tupinambá

    2014-02-01

    Full Text Available Trata-se de uma homenagem póstuma ao Professor Doutor Reinier Rozestraten. O texto apresenta, suscintamente, o percurso acadêmico do nomeado professor, em especial a sua passagem pela Psicologia do Trânsito. Paralelamente discorre sobre sua principal obra na área, que, de certo modo introduz o discurso psicológico sobre o comportamento de trânsito em perspectiva nacional, juntamente com sua preocupação em imprimir um caráter científico ao tema e introduzi-lo no contexto da academia e na sociedade em perspectivas teórica e prática. A obra escolhida é considerada um marco para o estabelecimento teórico da disciplina da psicologia do trânsito no Brasil. A homenagem foi originalmente escrita por ocasião dos cinco anos de falecimento do professor Reinier.

  19. Comparison of atmospheric CO2 mole fractions and source-sink characteristics at four WMO/GAW stations in China

    Science.gov (United States)

    Cheng, Siyang; Zhou, Lingxi; Tans, Pieter P.; An, Xingqin; Liu, Yunsong

    2018-05-01

    As CO2 is a primary driving factor of climate change, the mole fraction and source-sink characteristics of atmospheric CO2 over China are constantly inferred from multi-source and multi-site data. In this paper, we compared ground-based CO2 measurements with satellite retrievals and investigated the source-sink regional representativeness at China's four WMO/GAW stations. The results indicate that, firstly, atmospheric CO2 mole fractions from ground-based sampling measurement and Greenhouse Gases Observing Satellite (GOSAT) products reveal similar seasonal variation. The seasonal amplitude of the column-averaged CO2 mole fractions is smaller than that of the ground-based CO2 at all stations. The extrema of the seasonal cycle of ground-based and column CO2 mole fractions are basically synchronous except a slight phase delay at Lin'an (LAN) station. For the two-year average, the column CO2 is lower than ground-based CO2, and both of them reveal the lowest CO2 mole fraction at Waliguan (WLG) station. The lowest (∼4 ppm) and largest (∼8 ppm) differences between the column and ground-based CO2 appear at WLG and Longfengshan (LFS) stations, respectively. The CO2 mole fraction and its difference between GOSAT and ground-based measurement are smaller in summer than in winter. The differences of summer column CO2 among these stations are also much smaller than their ground-based counterparts. In winter, the maximum of ground-based CO2 mole fractions and the greatest difference between the two (ground-based and column) datasets appear at the LFS station. Secondly, the representative areas of the monthly CO2 background mole fractions at each station were found by employing footprints and emissions. Smaller representative areas appeared at Shangdianzi (SDZ) and LFS, whereas larger ones were seen at WLG and LAN. The representative areas in summer are larger than those in winter at WLG and SDZ, but the situation is opposite at LAN and LFS. The representative areas for the

  20. Sensibilidade da rosa 'Osiana' ao etileno

    Directory of Open Access Journals (Sweden)

    Delaine Cristina Cordeiro

    2011-01-01

    Full Text Available Os objetivos deste trabalho foram determinar a sensibilidade e as respostas da rosa cultivar Osiana (Rosa × hybrida à presença de etileno ao longo da vida pós-colheita. Para determinar a sensibilidade da flor ao etileno, hastes foram cortadas e colocadas em câmaras herméticas e em seguida tratadas por 24 horas com diferentes concentrações de etileno variando de 0,0; 0,1; 1,0; 10; 100 e 1000 µL L-1do ar no interior da câmara. A rosa teve a vida pós-colheita reduzida pelo etileno, quando exposta a concentração igual ou superior a 10 µL L-1 de etileno. As flores tratadas com no mínimo de 10 µL L-1de etileno desenvolveram necrose, murcha e abscisão prematura das pétalas ainda túrgidas. As concentrações de 0,1 e 1,0 µL L-1de etileno estimularam a abertura do botão floral durante o período de 24 horas de exposição ao etileno, sem no entanto, reduzir a vida pós-colheita. A rosa 'Osiana' pode ser classificada como uma cultivar sensível ao etileno e a concentração influência os processos estimulados.

  1. Expression patterns of ERVWE1/Syncytin-1 and other placentally expressed human endogenous retroviruses along the malignant transformation process of hydatidiform moles.

    Science.gov (United States)

    Bolze, Pierre-Adrien; Patrier, Sophie; Cheynet, Valérie; Oriol, Guy; Massardier, Jérôme; Hajri, Touria; Guillotte, Michèle; Bossus, Marc; Sanlaville, Damien; Golfier, François; Mallet, François

    2016-03-01

    Up to 20% of hydatidiform moles are followed by malignant transformation in gestational trophoblastic neoplasia and require chemotherapy. Syncytin-1 is involved in human placental morphogenesis and is also expressed in various cancers. We assessed the predictive value of the expression of Syncytin-1 and its interactants in the malignant transformation process of hydatidiform moles. Syncytin-1 glycoprotein was localized by immunohistochemistry in hydatidiform moles, gestational trophoblastic neoplasia and control placentas. The transcription levels of its locus ERVWE1, its interaction partners (hASCT1, hASCT2, TLR4 and DC-SIGN) and two loci (ERVFRDE1 and ERV3) involved the expression of other placental envelopes were assessed by real-time PCR. Syncytin-1 glycoprotein was expressed in syncytiotrophoblast of hydatidiform moles with an apical enhancement when compared with normal placentas. Moles with further malignant transformation had a higher staining intensity of Syncytin-1 surface unit C-terminus but the transcription level of its locus ERVWE1 was not different from that of moles with further remission and normal placentas. hASCT1 and TLR4, showed lower transcription levels in complete moles when compared to normal placentas. ERVWE1, ERVFRDE1 and ERV3 transcription was down-regulated in hydatidiform moles and gestational trophoblastic neoplasia. Variations of Syncytin-1 protein localization and down-regulation of hASCT1 and TLR4 transcription are likely to reflect altered functions of Syncytin-1 in the premalignant context of complete moles. The reduced transcription in gestational trophoblastic diseases of ERVWE1, ERVFRDE1 and ERV3, which expression during normal pregnancy is differentially regulated by promoter region methylation, suggest a joint dysregulation mechanism in malignant context. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Robo-AO M Dwarf Multiplicity Survey

    Science.gov (United States)

    Lamman, Claire; Baranec, Christoph; Berta-Thompson, Zachory K.; Law, Nicholas M.; Ziegler, Carl; Schonhut-Stasik, Jessica

    2018-06-01

    We analyzed close to 7,000 observations from Robo-AO’s field M dwarf survey taken on the 2.1m Kitt Peak telescope. Results will help determine the total multiplicity fraction and multiplicity functions of M dwarfs, which are crucial steps towards understanding their evolution and formation mechanics. Through its robotic, laser-guided, and automated system, the Robo-AO instrument has yielded the largest adaptive-optics M dwarf survey to date. I developed a graphical user interface to quickly analyze this data. Initial data analysis included assessing data quality, checking the result from Robo-AO’s automatic reduction pipeline, and determining existence as well as the relative position of companions through a visual inspection. This program can be applied to other datasets and was successfully tested by re-analyzing observations from a separate Robo-AO survey. After a conservative initial cut for quality, over 350 companions were found within 4” of a primary star out of 2,746 high quality Robo-AO M dwarf observations, including four triple systems. Further observations were done with the Keck II telescope by using its NIRC2 imager to follow up on ten select targets for the existence and physical association of companions. Future research will yield insights into low-mass stellar formation and provide a database of nearby M dwarf multiples that will potentially assist ongoing and future surveys for planets around these stars, such as the NASA TESS mission.

  3. High specific activity N-Acetyl-3H-α-Aspartyl- L-Glutamic at micro mole scale

    International Nuclear Information System (INIS)

    Suarez, C.

    1984-01-01

    High specific activity N-Acetyl-3 H - α -Aspartyl-I-Glutamic acid at micro mole scale in prepared acetylating L- α -Aspartyl-L-glutamic with 3 H -acetic anhydride in re distilled toluene. The product le purified through cationic and anionic columns. The radiochemical purity as determined by thin-layer chromatography is greater then 99% at the time preparation. (Author) 5 refs

  4. Rediscovery Of The Northern Mole Vole, Ellobius Talpinus (Rodentia, Cricetidae, At The Western Bank Of The Dnipro River, Ukraine

    Directory of Open Access Journals (Sweden)

    Rusin M.

    2015-06-01

    Full Text Available Ellobius talpinus was supposed to become extinct from the westernbank of the river Dnipro. Aft er 50 years the species was found again in Dnipropetrovsk and Kherson Region. The brief description of the current distribution of the northern mole vole to the west of the Dnipro is given. Altogether 11 localities were found. The rediscovered populations may be treated as endangered in the region.

  5. Reduced Utilization of Selenium by Naked Mole Rats Due to a Specific Defect in GPx1 Expression*

    OpenAIRE

    Kasaikina, Marina V.; Lobanov, Alexei V.; Malinouski, Mikalai Y.; Lee, Byung Cheon; Seravalli, Javier; Fomenko, Dmitri E.; Turanov, Anton A.; Finney, Lydia; Vogt, Stefan; Park, Thomas J.; Miller, Richard A.; Hatfield, Dolph L.; Gladyshev, Vadim N.

    2011-01-01

    Naked mole rat (MR) Heterocephalus glaber is a rodent model of delayed aging because of its unusually long life span (>28 years). It is also not known to develop cancer. In the current work, tissue imaging by x-ray fluorescence microscopy and direct analyses of trace elements revealed low levels of selenium in the MR liver and kidney, whereas MR and mouse brains had similar selenium levels. This effect was not explained by uniform selenium deficiency because methionine sulfoxide reductase act...

  6. Challenging the inbreeding hypothesis in a eusocial mammal: population genetics of the naked mole-rat, Heterocephalus glaber.

    Science.gov (United States)

    Ingram, Colleen M; Troendle, Nicholas J; Gill, Clare A; Braude, Stanton; Honeycutt, Rodney L

    2015-10-01

    The role of genetic relatedness in the evolution of eusociality has been the topic of much debate, especially when contrasting eusocial insects with vertebrates displaying reproductive altruism. The naked mole-rat, Heterocephalus glaber, was the first described eusocial mammal. Although this discovery was based on an ecological constraints model of eusocial evolution, early genetic studies reported high levels of relatedness in naked mole-rats, providing a compelling argument that low dispersal rates and consanguineous mating (inbreeding as a mating system) are the driving forces for the evolution of this eusocial species. One caveat to accepting this long-held view is that the original genetic studies were based on limited sampling from the species' geographic distribution. A growing body of evidence supports a contrary view, with the original samples not representative of the species-rather reflecting a single founder event, establishing a small population south of the Athi River. Our study is the first to address these competing hypotheses by examining patterns of molecular variation in colonies sampled from north and south of the Athi and Tana rivers, which based on our results, serve to isolate genetically distinct populations of naked mole-rats. Although colonies south of the Athi River share a single mtDNA haplotype and are fixed at most microsatellite loci, populations north of the Athi River are considerably more variable. Our findings support the position that the low variation observed in naked mole-rat populations south of the Athi River reflects a founder event, rather than a consequence of this species' unusual mating system. © 2015 John Wiley & Sons Ltd.

  7. The Insulin-Like Growth Factor System in the Long-Lived Naked Mole-Rat.

    Directory of Open Access Journals (Sweden)

    Malene Brohus

    Full Text Available Naked mole-rats (Heterocephalus glaber (NMRs are the longest living rodents known. They show negligible senescence, and are resistant to cancers and certain damaging effects associated with aging. The insulin-like growth factors (IGFs have pluripotent actions, influencing growth processes in virtually every system of the body. They are established contributors to the aging process, confirmed by the demonstration that decreased IGF signaling results in life-extending effects in a variety of species. The IGFs are likewise involved in progression of cancers by mediating survival signals in malignant cells. This report presents a full characterization of the IGF system in the NMR: ligands, receptors, IGF binding proteins (IGFBPs, and IGFBP proteases. A particular emphasis was placed on the IGFBP protease, pregnancy-associated plasma protein-A (PAPP-A, shown to be an important lifespan modulator in mice. Comparisons of IGF-related genes in the NMR with human and murine sequences indicated no major differences in essential parts of the IGF system, including PAPP-A. The protease was shown to possess an intact active site despite the report of a contradictory genome sequence. Furthermore, PAPP-A was expressed and translated in NMRs cells and retained IGF-dependent proteolytic activity towards IGFBP-4 and IGF-independent activity towards IGFBP-5. However, experimental data suggest differential regulatory mechanisms for PAPP-A expression in NMRs than those described in humans and mice. This overall description of the IGF system in the NMR represents an initial step towards elucidating the complex molecular mechanisms underlying longevity, and how these animals have evolved to ensure a delayed and healthy aging process.

  8. Initial Case Reports of Cancer in Naked Mole-rats (Heterocephalus glaber).

    Science.gov (United States)

    Delaney, M A; Ward, J M; Walsh, T F; Chinnadurai, S K; Kerns, K; Kinsel, M J; Treuting, P M

    2016-05-01

    Naked mole-rats (NMRs;Heterocephalus glaber) are highly adapted, eusocial rodents renowned for their extreme longevity and resistance to cancer. Because cancer has not been formally described in this species, NMRs have been increasingly utilized as an animal model in aging and cancer research. We previously reported the occurrence of several age-related diseases, including putative pre-neoplastic lesions, in zoo-housed NMR colonies. Here, we report for the first time 2 cases of cancer in zoo-housed NMRs. In Case No. 1, we observed a subcutaneous mass in the axillary region of a 22-year-old male NMR, with histologic, immunohistochemical (pancytokeratin positive, rare p63 immunolabeling, and smooth muscle actin negative), and ultrastructural characteristics of an adenocarcinoma possibly of mammary or salivary origin. In Case No. 2, we observed a densely cellular, poorly demarcated gastric mass of polygonal cells arranged in nests with positive immunolabeling for synaptophysin and chromogranin indicative of a neuroendocrine carcinoma in an approximately 20-year-old male NMR. We also include a brief discussion of other proliferative growths and pre-cancerous lesions diagnosed in 1 zoo colony. Although these case reports do not alter the longstanding observation of cancer resistance, they do raise questions about the scope of cancer resistance and the interpretation of biomedical studies in this model. These reports also highlight the benefit of long-term disease investigations in zoo-housed populations to better understand naturally occurring disease processes in species used as models in biomedical research. © The Author(s) 2016.

  9. The Insulin-Like Growth Factor System in the Long-Lived Naked Mole-Rat.

    Science.gov (United States)

    Brohus, Malene; Gorbunova, Vera; Faulkes, Chris G; Overgaard, Michael T; Conover, Cheryl A

    2015-01-01

    Naked mole-rats (Heterocephalus glaber) (NMRs) are the longest living rodents known. They show negligible senescence, and are resistant to cancers and certain damaging effects associated with aging. The insulin-like growth factors (IGFs) have pluripotent actions, influencing growth processes in virtually every system of the body. They are established contributors to the aging process, confirmed by the demonstration that decreased IGF signaling results in life-extending effects in a variety of species. The IGFs are likewise involved in progression of cancers by mediating survival signals in malignant cells. This report presents a full characterization of the IGF system in the NMR: ligands, receptors, IGF binding proteins (IGFBPs), and IGFBP proteases. A particular emphasis was placed on the IGFBP protease, pregnancy-associated plasma protein-A (PAPP-A), shown to be an important lifespan modulator in mice. Comparisons of IGF-related genes in the NMR with human and murine sequences indicated no major differences in essential parts of the IGF system, including PAPP-A. The protease was shown to possess an intact active site despite the report of a contradictory genome sequence. Furthermore, PAPP-A was expressed and translated in NMRs cells and retained IGF-dependent proteolytic activity towards IGFBP-4 and IGF-independent activity towards IGFBP-5. However, experimental data suggest differential regulatory mechanisms for PAPP-A expression in NMRs than those described in humans and mice. This overall description of the IGF system in the NMR represents an initial step towards elucidating the complex molecular mechanisms underlying longevity, and how these animals have evolved to ensure a delayed and healthy aging process.

  10. Transcriptome sequencing of the naked mole rat (Heterocephalus glaber and identification of hypoxia tolerance genes

    Directory of Open Access Journals (Sweden)

    Bang Xiao

    2017-12-01

    Full Text Available The naked mole rat (NMR; Heterocephalus glaber is a small rodent species found in regions of Kenya, Ethiopia and Somalia. It has a high tolerance for hypoxia and is thus considered one of the most important natural models for studying hypoxia tolerance mechanisms. The various mechanisms underlying the NMR's hypoxia tolerance are beginning to be understood at different levels of organization, and next-generation sequencing methods promise to expand this understanding to the level of gene expression. In this study, we examined the sequence and transcript abundance data of the muscle transcriptome of NMRs exposed to hypoxia using the Illumina HiSeq 2500 system to clarify the possible genomic adaptive responses to the hypoxic underground surroundings. The RNA-seq raw FastQ data were mapped against the NMR genome. We identified 2337 differentially expressed genes (DEGs by comparison of the hypoxic and control groups. Functional annotation of the DEGs by gene ontology (GO analysis revealed enrichment of hypoxia stress-related GO categories, including ‘biological regulation’, ‘cellular process’, ‘ion transport’ and ‘cell-cell signaling’. Enrichment of DEGs in signaling pathways was analyzed against the Kyoto Encyclopedia of Genes and Genomes (KEGG database to identify possible interactions between DEGs. The results revealed significant enrichment of DEGs in focal adhesion, the mitogen-activated protein kinase (MAPK signaling pathway and the glycine, serine and threonine metabolism pathway. Furthermore, inhibition of DEGs (STMN1, MAPK8IP1 and MAPK10 expression induced apoptosis and arrested cell growth in NMR fibroblasts following hypoxia. Thus, this global transcriptome analysis of NMRs can provide an important genetic resource for the study of hypoxia tolerance in mammals. Furthermore, the identified DEGs may provide important molecular targets for biomedical research into therapeutic strategies for stroke and cardiovascular diseases.

  11. A new definition for the mole based on the Avogadro constant: a journey from physics to chemistry.

    Science.gov (United States)

    Milton, Martin J T

    2011-10-28

    The mole is the most recent addition to the set of base units that form the International System of Units, although its pre-cursor the 'gram-molecule', had been in use by both physicists and chemists for more than 120 years. A proposal has been published recently to establish a new definition for the mole based on a fixed value for the Avogadro constant. This would introduce consistent relative uncertainties for the molar and the atomic masses while making no change to the system of relative atomic masses ('atomic weights'). Although the proposal would have little impact on the measurement uncertainty of practical work, it has stimulated considerable debate about the mole and the nature of the quantity amount of substance. In this paper, the rationale for the new definition is explained against the background of changes in the way the quantity amount of substance has been used, from its first use during the early development of thermodynamics through to the use of the 'number of gram-molecules' at the end of the nineteenth century.

  12. Naked mole-rats maintain healthy skeletal muscle and Complex IV mitochondrial enzyme function into old age.

    Science.gov (United States)

    Stoll, Elizabeth A; Karapavlovic, Nevena; Rosa, Hannah; Woodmass, Michael; Rygiel, Karolina; White, Kathryn; Turnbull, Douglass M; Faulkes, Chris G

    2016-12-19

    The naked mole-rat (NMR) Heterocephalus glaber is an exceptionally long-lived rodent, living up to 32 years in captivity. This extended lifespan is accompanied by a phenotype of negligible senescence, a phenomenon of very slow changes in the expected physiological characteristics with age. One of the many consequences of normal aging in mammals is the devastating and progressive loss of skeletal muscle, termed sarcopenia, caused in part by respiratory enzyme dysfunction within the mitochondria of skeletal muscle fibers. Here we report that NMRs avoid sarcopenia for decades. Muscle fiber integrity and mitochondrial ultrastructure are largely maintained in aged animals. While mitochondrial Complex IV expression and activity remains stable, Complex I expression is significantly decreased. We show that aged naked mole-rat skeletal muscle tissue contains some mitochondrial DNA rearrangements, although the common mitochondrial DNA deletions associated with aging in human and other rodent skeletal muscles are not present. Interestingly, NMR skeletal muscle fibers demonstrate a significant increase in mitochondrial DNA copy number. These results have intriguing implications for the role of mitochondria in aging, suggesting Complex IV, but not Complex I, function is maintained in the long-lived naked mole rat, where sarcopenia is avoided and healthy muscle function is maintained for decades.

  13. Cessation of reproduction-related spine elongation after multiple breeding cycles in female naked mole-rats.

    Science.gov (United States)

    Dengler-Crish, Christine M; Catania, Kenneth C

    2009-01-01

    The breeding female or "queen" naked mole-rat has a uniquely elongated body morphology attributed to the lengthening of the lumbar vertebral column that occurs during pregnancy. It is unknown whether this vertebral growth is a continuous process, or associated only with early reproductive experience. We compared pregnancy-related bone elongation in nascent primiparous queens and established queens to determine if this vertebral expansion was a lifelong process in these females. We also investigated the impact of lactation on vertebral elongation in these mole-rats because it is known to be a time of significant bone loss in other mammals. Our data show that after eight or more pregnancies, established queens no longer experienced a net gain in lumbar spine length over the reproductive cycle, whereas the nascent breeders demonstrated significant spine lengthening over this time. Despite the lack of net spine lengthening in established breeders, our results indicated that these queens still experienced some pregnancy-specific vertebral elongation. In naked mole-rats, pregnancy-induced bone elongation may serve the dual purposes of first lengthening the spine, and then once optimal spine size is achieved, serving as a homeostatic mechanism that prepares the spine for the mineral demands of lactation. Anat Rec, 2009. (c) 2008 Wiley-Liss, Inc.

  14. Retinal S-opsin dominance in Ansell's mole-rats (Fukomys anselli) is a consequence of naturally low serum thyroxine.

    Science.gov (United States)

    Henning, Yoshiyuki; Mladěnková, Nella; Burda, Hynek; Szafranski, Karol; Begall, Sabine

    2018-03-12

    Mammals usually possess a majority of medium-wavelength sensitive (M-) and a minority of short-wavelength sensitive (S-) opsins in the retina, enabling dichromatic vision. Unexpectedly, subterranean rodents from the genus Fukomys exhibit an S-opsin majority, which is exceptional among mammals, albeit with no apparent adaptive value. Because thyroid hormones (THs) are pivotal for M-opsin expression and metabolic rate regulation, we have, for the first time, manipulated TH levels in the Ansell's mole-rat (Fukomys anselli) using osmotic pumps. In Ansell's mole-rats, the TH thyroxine (T4) is naturally low, likely as an adaptation to the harsh subterranean ecological conditions by keeping resting metabolic rate (RMR) low. We measured gene expression levels in the eye, RMR, and body mass (BM) in TH-treated animals. T4 treatment increased both, S- and M-opsin expression, albeit M-opsin expression at a higher degree. However, this plasticity was only given in animals up to approximately 2.5 years. Mass-specific RMR was not affected following T4 treatment, although BM decreased. Furthermore, the T4 inactivation rate is naturally higher in F. anselli compared to laboratory rodents. This is the first experimental evidence that the S-opsin majority in Ansell's mole-rats is a side effect of low T4, which is downregulated to keep RMR low.

  15. Non-invasive assessment of glucocorticoid and androgen metabolite levels in cooperatively breeding Damaraland mole-rats (Fukomys damarensis).

    Science.gov (United States)

    Medger, Katarina; Bennett, Nigel C; Lutermann, Heike; Ganswindt, Andre

    2018-05-18

    Dominant females of cooperative breeding species often use aggression to suppress reproduction of subordinate females, resulting in subordinates experiencing stress-related increases in glucocorticoid levels, which may cause reproductive down-regulation. This would suggest a general pattern with higher glucocorticoid levels in subordinate compared to dominant individuals; however, the opposite was found in a number of cooperatively breeding species. Furthermore, breeding females of the cooperatively breeding Damaraland mole-rats (Fukomys damarensis) exhibit very high androgen concentrations during the wet season, presumably to support their breeding monopoly. Hormone analysis in Damaraland mole-rats have typically been measured using plasma and urine, but faecal analysis offers additional advantages especially for field studies on this species. The present study examines the suitability of Damaraland mole-rat faecal samples for determining glucocorticoid metabolite (fGCM) and androgen metabolite (fAM) concentrations using enzyme immunoassays. Using these assays, we further evaluated the effects of breeding status on fGCM and fAM concentrations in wild-caught and captive Damaraland mole-rats. Wild-caught breeding and non-breeding males and females exhibited no differences in fAM concentrations. Immunoreactive fGCM concentrations were only high in male breeders and comparatively low in non-breeders and breeding females. Concentrations of fAMs and fGCMs were similar in captive males and females, but fAM concentrations were elevated in captive compared to wild-caught individuals, which may be related to a higher reproductive activity due to removal from the breeding female. The relatively uniform fAM and fGCM concentrations found in wild-caught mole-rats may be explained by a stable colony structure during the dry season during which this study was conducted. Limited dispersal opportunities result in lower aggression and stress levels within a colony and as a result

  16. ROBO-AO M DWARF MULTIPLICITY SURVEY

    Science.gov (United States)

    Lamman, Claire; Berta-Thompson, Zachory; Baranec, Christoph; Law, Nicholas; Schonhut, Jessica

    2018-01-01

    We analyzed over 7,000 observations from Robo-AO’s field M dwarf survey taken on the 2.1m Kitt Peak telescope. Results will help determine the multiplicity fraction of M dwarfs as a function of primary mass, which is a crucial step towards understanding their evolution and formation mechanics. Through its robotic, laser-guided, and automated system, the Robo-AO instrument has yielded the largest adaptive-optics M dwarf survey to date. I developed a graphical user interface to quickly analyze this data. Initial data analysis included assessing data quality, checking the result from Robo-AO’s automatic reduction pipeline, and determining existence as well as the relative position of companions through a visual inspection. This program can be applied to other datasets and was successfully tested by re-analyzing observations from a separate Robo-AO survey. Following the preliminary results from this data analysis tool, further observations were done with the Keck II telescope by using its NIRC2 imager to follow up on ten select targets for the existence and physical association of companions. After a conservative initial cut for quality, 356 companions were found within 4” of a primary star out of 2,746 high quality Robo-AO M dwarf observations, including four triple systems. We will present a preliminary estimate for the multiplicity rate of wide M dwarf companions after accounting for observation limitations and the completeness of our search. Future research will yield insights into low-mass stellar formation and provide a database of nearby M dwarf multiples that will potentially assist ongoing and future surveys for planets around these stars, such as the NASA TESS mission.

  17. Hepatotoxicidade associada ao uso de esteroides anabolizantes

    OpenAIRE

    Mónica Brinquinho; Ana Andresa Sousa; Janine Resende; João Valente

    2017-01-01

    O consumo de esteroides androgénicos anabolizantes tem vindo a aumentar no meio desportivo amador, sendo de fácil acesso apesar de se tratarem de substâncias proibidas. Estão descritos múltiplos efeitos laterais, sendo a hepatotoxicidade uma das mais reportadas. Apresentam-se dois casos clínicos representativos da lesão hepática associada ao consumo de esteroides anabolizantes. Ambos evidenciaram morbilidade significativa, embora nenhum deles tenha evoluído para falência hepática. Os autores ...

  18. Geochemical and Petrologic Constraints on the Source of Eocene Volcanism at Mole Hill, Rockingham County, VA

    Science.gov (United States)

    Johnson, E. A.; Beard, J. S.

    2010-12-01

    Mole Hill is an Eocene (48 Ma) basaltic volcanic neck located west of Harrisonburg, VA, and provides a unique opportunity to probe the mantle beneath the Shenandoah Valley. It lies on the northeastern edge of a swarm of alkaline-series volcanic plugs, dikes, and diatremes extending through Rockingham and Highland Counties, VA, and Pendleton County, WV. The Eocene volcanics are thought to have exploited extensive basement fracture systems originally formed during the Alleghenian Orogeny and subsequent rifting. The Eocene volcanism may have been triggered by reactivation of faults due to global shifts in relative plate motions (Southworth 1993, USGS Bull, B1839-I) but the source material and magmatic processes for the Eocene volcanism are largely unknown. Compositional and texture analyses of xenocrystic and groundmass clinopyroxene, olivine, and spinel were completed either at Virginia Tech on the Cameca SX-50 electron microprobe in the Dept of Geological Sciences, or in the Dept of Mineral Sciences, Smithsonian Institution, Washington, D.C using the JEOL JXA-8900R WD/EDS microanalyzer or the FEI NOVA nanoSEM600 FEG Variable Pressure Scanning Electron Microscope. Xenocrysts up to 2cm in diameter are distributed throughout the volcanic neck, with clinopyroxene >>spinel>olivine. The clinopyroxene and olivine xenocrysts show undulatory extinction in cross-polarized light and are found as individual crystals or as aggregates. Clinopyroxene xenocryst cores are high-Al, low-Cr augite ( ˜Wo44En46Fs10) with Mg# 78.5-85.9. The clinopyroxene xenocrysts have compositionally zoned rims 100-250 μm-wide containing abundant plagioclase inclusions and sparse melt inclusions in a sieve texture. The outer edges of xenocrysts approach the compositions of groundmass and microphenocryst clinopyroxenes ( ˜Wo47En38Fs15; Mg# 67.9-74.5). Olivine xenocrysts contain sulfide inclusions and Cr-rich spinel and have Mg-rich ( ˜Fo86-90) cores with more Fe- and Ca-rich rims (Fo70

  19. Membrane phospholipid composition may contribute to exceptional longevity of the naked mole-rat (Heterocephalus glaber): a comparative study using shotgun lipidomics.

    Science.gov (United States)

    Mitchell, Todd W; Buffenstein, Rochelle; Hulbert, A J

    2007-11-01

    Phospholipids containing highly polyunsaturated fatty acids are particularly prone to peroxidation and membrane composition may therefore influence longevity. Phospholipid molecules, in particular those containing docosahexaenoic acid (DHA), from the skeletal muscle, heart, liver and liver mitochondria were identified and quantified using mass-spectrometry shotgun lipidomics in two similar-sized rodents that show an approximately 9-fold difference in maximum lifespan. The naked mole rat is the longest-living rodent known with a maximum lifespan of >28 years. Total phospholipid distribution is similar in tissues of both species; DHA is only found in phosphatidylcholines (PC), phosphatidylethanolamines (PE) and phosphatidylserines (PS), and DHA is relatively more concentrated in PE than PC. Naked mole-rats have fewer molecular species of both PC and PE than do mice. DHA-containing phospholipids represent 27-57% of all phospholipids in mice but only 2-6% in naked mole-rats. Furthermore, while mice have small amounts of di-polyunsaturated PC and PE, these are lacking in naked mole-rats. Vinyl ether-linked phospholipids (plasmalogens) are higher in naked mole-rat tissues than in mice. The lower level of DHA-containing phospholipids suggests a lower susceptibility to peroxidative damage in membranes of naked mole-rats compared to mice. Whereas the high level of plasmalogens might enhance membrane antioxidant protection in naked mole-rats compared to mice. Both characteristics possibly contribute to the exceptional longevity of naked mole-rats and may indicate a special role for peroxisomes in this extended longevity.

  20. Experimental Investigation of InSight HP3 Mole Interaction with Martian Regolith Simulant. Quasi-Static and Dynamic Penetration Testing

    Science.gov (United States)

    Marshall, Jason P.; Hudson, Troy L.; Andrade, José E.

    2017-10-01

    The InSight mission launches in 2018 to characterize several geophysical quantities on Mars, including the heat flow from the planetary interior. This quantity will be calculated by utilizing measurements of the thermal conductivity and the thermal gradient down to 5 meters below the Martian surface. One of the components of InSight is the Mole, which hammers into the Martian regolith to facilitate these thermal property measurements. In this paper, we experimentally investigated the effect of the Mole's penetrating action on regolith compaction and mechanical properties. Quasi-static and dynamic experiments were run with a 2D model of the 3D cylindrical mole. Force resistance data was captured with load cells. Deformation information was captured in images and analyzed using Digitial Image Correlation (DIC). Additionally, we used existing approximations of Martian regolith thermal conductivity to estimate the change in the surrounding granular material's thermal conductivity due to the Mole's penetration. We found that the Mole has the potential to cause a high degree of densification, especially if the initial granular material is relatively loose. The effect on the thermal conductivity from this densification was found to be relatively small in first-order calculations though more complete thermal models incorporating this densification should be a subject of further investigation. The results obtained provide an initial estimate of the Mole's impact on Martian regolith thermal properties.

  1. Naked mole-rat has increased translational fidelity compared with the mouse, as well as a unique 28S ribosomal RNA cleavage.

    Science.gov (United States)

    Azpurua, Jorge; Ke, Zhonghe; Chen, Iris X; Zhang, Quanwei; Ermolenko, Dmitri N; Zhang, Zhengdong D; Gorbunova, Vera; Seluanov, Andrei

    2013-10-22

    The naked mole-rat (Heterocephalus glaber) is a subterranean eusocial rodent with a markedly long lifespan and resistance to tumorigenesis. Multiple data implicate modulation of protein translation in longevity. Here we report that 28S ribosomal RNA (rRNA) of the naked mole-rat is processed into two smaller fragments of unequal size. The two breakpoints are located in the 28S rRNA divergent region 6 and excise a fragment of 263 nt. The excised fragment is unique to the naked mole-rat rRNA and does not show homology to other genomic regions. Because this hidden break site could alter ribosome structure, we investigated whether translation rate and amino acid incorporation fidelity were altered. We report that naked mole-rat fibroblasts have significantly increased translational fidelity despite having comparable translation rates with mouse fibroblasts. Although we cannot directly test whether the unique 28S rRNA structure contributes to the increased fidelity of translation, we speculate that it may change the folding or dynamics of the large ribosomal subunit, altering the rate of GTP hydrolysis and/or interaction of the large subunit with tRNA during accommodation, thus affecting the fidelity of protein synthesis. In summary, our results show that naked mole-rat cells produce fewer aberrant proteins, supporting the hypothesis that the more stable proteome of the naked mole-rat contributes to its longevity.

  2. Effect of the Ti/B4C mole ratio on the reaction products and reaction mechanism in an Al–Ti–B4C powder mixture

    International Nuclear Information System (INIS)

    Zhang, Jingjing; Lee, Jung-Moo; Cho, Young-Hee; Kim, Su-Hyeon; Yu, Huashun

    2014-01-01

    The effect of the Ti/B 4 C mole ratio on the fabrication behavior of Al composites is investigated using Al–Ti–B 4 C powder mixtures as reactants. The quick spontaneous infiltration (QSI) process combined with the combustion reaction and DTA analysis were used. According to the thermodynamic predictions, which are verified in the experimental results, TiB 2 is formed in all the samples whereas TiC is only formed in reactants with a Ti/B 4 C mole ratio of more than two. The C atoms from the reacted B 4 C do not move into TiC but instead they move into Al 3 BC or Al 4 C 3 when the Ti/B 4 C mole ratio is less than two. In addition, the reaction mechanism with a Ti/B 4 C mole ratio of 0.75 is investigated extensively. - Highlights: • The critical role of the Ti/B 4 C mole ratio on the reaction products of Al–Ti–B 4 C was studied using experiments. • The experimental results are also supported by thermodynamic calculations presented in this paper. • The reaction mechanism with a Ti/B 4 C mole ratio of 0.75 is investigated extensively

  3. Escala de valores relativos ao trabalho : EVT

    Directory of Open Access Journals (Sweden)

    Porto Juliana Barreiros

    2003-01-01

    Full Text Available Na literatura internacional são encontradas várias escalas para medir valores relativos ao trabalho, porém a maioria apresenta problemas com relação à definição do conceito ou a falta de integração com os modelos teóricos sobre valores pessoais. No Brasil foram encontrados dois instrumentos, um não foi validado para a população brasileira e o outro foi validado para uma população de baixa escolaridade. Foi objetivo do presente estudo desenvolver e validar uma Escala de Valores relativos ao Trabalho. Para o desenvolvimento da EVT foi realizado um levantamento de instrumentos anteriores e foram entrevistados trabalhadores. Após a análise de juízes e validação semântica, o instrumento foi respondido por 394 pessoas. A análise fatorial apontou para quatro fatores: Realização no trabalho, Relações sociais, Prestígio e Estabilidade. O instrumento após validação é composto por 45 itens. Os resultados corroboraram o modelo teórico previsto e conclui-se que a escala foi devidamente estabelecida e pode ser utilizada para pesquisa e diagnóstico.

  4. Nefrocalcinose associada ao uso de esteroide anabolizante

    Directory of Open Access Journals (Sweden)

    Weverton Machado Luchi

    2015-03-01

    Full Text Available Os esteroides anabolizantes têm sido usados como arma terapêutica em diversas condições clínicas. Entretanto, o uso abusivo e indiscriminado, associado a outros suplementos nutricionais, tem gerado efeitos adversos graves. Relato do caso: Sexo masculino, 21 anos, admitido com náuseas, astenia, hiporexia, cefaleia e hipertensão arterial. Exames no sangue evidenciaram Cr: 3,9 mg/dl U:100 mg/dl e Cálcio total 14 mg/dl. Ultrassonografia e biópsia renal compatíveis com nefrocalcinose. Houve melhora gradativa da função renal e da calcemia após hidratação vigorosa e furosemida. Entretanto, após 1 ano, persistiram depósitos renais de cálcio e relação córticomedular reduzida ao ultrassom e creatinina estável em 1,4 mg/dl. Casos anteriores evidenciaram necrose tubular aguda e nefrite intersticial com poucos depósitos de cálcio no interstício renal. Nesse caso, encontramos nefrocalcinose acentuada associada à nefroesclerose. O objetivo deste estudo é relatar a ocorrência de injúria renal aguda com nefrocalcinose associada ao uso de esteroide anabolizante e oferecer uma revisão do assunto.

  5. CONTRIBUIÇÃO AO ESTUDO TECNOLÓGICO DA CANELA-AZEITONA, Rapanea ferruginea (Ruiz e Pav. Mez, UMA ESPÉCIE PIONEIRA. I - CARACTERÍSTICAS ANATÔMICAS DA MADEIRA

    Directory of Open Access Journals (Sweden)

    Ana Paula Texeira do Carmo

    1993-12-01

    Full Text Available São descritos neste trabalho os aspectos anatômicos da madeira de Rapanea ferruginea (Ruiz et Pav. Mez. Apresentam-se dados quantitativos de 11 caracteres do xilema secundário, bem como fotomicrografias das estruturas anatômicas da madeira. Os raios largos e a largos e a baixa percentagem de parênquima axial são os caracteres mais importantes na estrutura do lenho.

  6. Unusual ratio between free thyroxine and free triiodothyronine in a long-lived mole-rat species with bimodal ageing.

    Science.gov (United States)

    Henning, Yoshiyuki; Vole, Christiane; Begall, Sabine; Bens, Martin; Broecker-Preuss, Martina; Sahm, Arne; Szafranski, Karol; Burda, Hynek; Dammann, Philip

    2014-01-01

    Ansell's mole-rats (Fukomys anselli) are subterranean, long-lived rodents, which live in eusocial families, where the maximum lifespan of breeders is twice as long as that of non-breeders. Their metabolic rate is significantly lower than expected based on allometry, and their retinae show a high density of S-cone opsins. Both features may indicate naturally low thyroid hormone levels. In the present study, we sequenced several major components of the thyroid hormone pathways and analyzed free and total thyroxine and triiodothyronine in serum samples of breeding and non-breeding F. anselli to examine whether a) their thyroid hormone system shows any peculiarities on the genetic level, b) these animals have lower hormone levels compared to euthyroid rodents (rats and guinea pigs), and c) reproductive status, lifespan and free hormone levels are correlated. Genetic analyses confirmed that Ansell's mole-rats have a conserved thyroid hormone system as known from other mammalian species. Interspecific comparisons revealed that free thyroxine levels of F. anselli were about ten times lower than of guinea pigs and rats, whereas the free triiodothyronine levels, the main biologically active form, did not differ significantly amongst species. The resulting fT4:fT3 ratio is unusual for a mammal and potentially represents a case of natural hypothyroxinemia. Comparisons with total thyroxine levels suggest that mole-rats seem to possess two distinct mechanisms that work hand in hand to downregulate fT4 levels reliably. We could not find any correlation between free hormone levels and reproductive status, gender or weight. Free thyroxine may slightly increase with age, based on sub-significant evidence. Hence, thyroid hormones do not seem to explain the different ageing rates of breeders and non-breeders. Further research is required to investigate the regulatory mechanisms responsible for the unusual proportion of free thyroxine and free triiodothyronine.

  7. Unusual ratio between free thyroxine and free triiodothyronine in a long-lived mole-rat species with bimodal ageing.

    Directory of Open Access Journals (Sweden)

    Yoshiyuki Henning

    Full Text Available Ansell's mole-rats (Fukomys anselli are subterranean, long-lived rodents, which live in eusocial families, where the maximum lifespan of breeders is twice as long as that of non-breeders. Their metabolic rate is significantly lower than expected based on allometry, and their retinae show a high density of S-cone opsins. Both features may indicate naturally low thyroid hormone levels. In the present study, we sequenced several major components of the thyroid hormone pathways and analyzed free and total thyroxine and triiodothyronine in serum samples of breeding and non-breeding F. anselli to examine whether a their thyroid hormone system shows any peculiarities on the genetic level, b these animals have lower hormone levels compared to euthyroid rodents (rats and guinea pigs, and c reproductive status, lifespan and free hormone levels are correlated. Genetic analyses confirmed that Ansell's mole-rats have a conserved thyroid hormone system as known from other mammalian species. Interspecific comparisons revealed that free thyroxine levels of F. anselli were about ten times lower than of guinea pigs and rats, whereas the free triiodothyronine levels, the main biologically active form, did not differ significantly amongst species. The resulting fT4:fT3 ratio is unusual for a mammal and potentially represents a case of natural hypothyroxinemia. Comparisons with total thyroxine levels suggest that mole-rats seem to possess two distinct mechanisms that work hand in hand to downregulate fT4 levels reliably. We could not find any correlation between free hormone levels and reproductive status, gender or weight. Free thyroxine may slightly increase with age, based on sub-significant evidence. Hence, thyroid hormones do not seem to explain the different ageing rates of breeders and non-breeders. Further research is required to investigate the regulatory mechanisms responsible for the unusual proportion of free thyroxine and free triiodothyronine.

  8. Social status and sex independently influence androgen receptor expression in the eusocial naked mole-rat brain.

    Science.gov (United States)

    Holmes, Melissa M; Goldman, Bruce D; Forger, Nancy G

    2008-08-01

    Naked mole-rats (Heterocephalus glaber) are eusocial rodents that live in large subterranean colonies including a single breeding female and 1-3 breeding males; all other members of the colony, known as subordinates, are reproductively suppressed. We recently found that naked mole-rats lack many of the sex differences in the brain and spinal cord commonly found in other rodents. Instead, neural morphology is influenced by breeding status, such that breeders, regardless of sex, have more neurons than subordinates in the ventromedial nucleus of the hypothalamus (VMH), and larger overall volumes of the bed nucleus of the stria terminalis (BST), paraventricular nucleus (PVN) and medial amygdala (MeA). To begin to understand how breeding status influences brain morphology, we examined the distribution of androgen receptor (AR) immunoreactivity in gonadally intact breeders and subordinates of both sexes. All animals had AR+ nuclei in many of the same regions positive for AR in other mammals, including the VMH, BST, PVN, MeA, and the ventral portion of the premammillary nucleus (PMv). We also observed diffuse labeling throughout the preoptic area, demonstrating that distribution of the AR protein in presumptive reproductive brain nuclei is well-conserved, even in a species that exhibits remarkably little sexual dimorphism. In contrast to other rodents, however, naked mole-rats lacked AR+ nuclei in the suprachiasmatic nucleus and hippocampus. Males had more AR+ nuclei in the MeA, VMH, and PMv than did females. Surprisingly, breeders had significantly fewer AR+ nuclei than subordinates in all brain regions examined (VMH, BST, PVN, MeA, and PMv). Thus, social status is strongly correlated with AR immunoreactivity in this eusocial species.

  9. Postprandial heat increment does not substitute for active thermogenesis in cold-challenged star-nosed moles (Condylura cristata).

    Science.gov (United States)

    Campbell, K L; McIntyre, I W; MacArthur, R A

    2000-01-01

    The postprandial increase in metabolic rate associated with consuming, assimilating and excreting a meal is often termed the heat increment of feeding (HIF). The metabolic heat production of star-nosed moles, Condylura cristata, held at thermoneutrality was monitored for 4 h following a single 10 min session of feeding on a ration consisting of 0 g (controls), 3.5 g or 10 g of earthworms. Coefficients for metabolizable energy digestibility and digesta passage rate of earthworms fed to C. cristata were also determined. We then tested whether feeding-induced thermogenesis substitutes partially or completely for thermoregulatory heat production in these animals exposed to sub-thermoneutral air temperatures (9-24 degrees C). A single feeding on earthworms had both short- and long-term effects on the metabolic rate and respiratory exchange ratio of C. cristata. The observed short-term (0-65 min) rise in metabolic rate, assumed to be associated primarily with the physical costs of nutrient digestion, absorption and excretion, was similar to the calculated mean retention time (66.7+/-7.8 min; mean +/- s.e. m., N=5) of this species. This component of the HIF represented 2.9 % of the food energy ingested by moles fed a single 3.5 g (13.21 kJ) meal of earthworms and 1.4 % of the food energy ingested by moles fed a single 7.5 g (28.09 kJ) meal of earthworms. At all test temperatures, resting metabolic rate typically remained above fasting levels for 1-4 h following ingestion of the high-protein earthworm diet. This protracted rise in metabolic rate, presumably associated with the biochemical costs of amino acid oxidation/gluconeogenesis and ureagenesis, averaged 12.8 % of the metabolizable energy and 8.7 % of the gross energy intake. Despite the potential thermoregulatory benefit, we found no evidence that biochemical HIF substitutes for facultative thermogenesis in star-nosed moles exposed to low air temperatures.

  10. Diffusion coefficients in 4-component mixture expressed explicitly in terms of binary diffusion coefficients and mole fractions

    International Nuclear Information System (INIS)

    Furuta, Hiroshi; Yamamoto, Ichiro

    1996-01-01

    Diffusion coefficients in 4-component mixture D ij (4) were expressed explicitly in terms of binary diffusion coefficients and mole fractions by solving a ratio of determinants defined by Hirschfelder et al. The explicit expressions of D ij (4) were divided into two terms, a term due to the i-j pairs of attention and a term common to all the pairs out of the 4 components. The two terms of D ij (4) had extended structures similar to corresponding those of D ij (3) respectively. (author)

  11. Hepatotoxicidade associada ao uso de esteroides anabolizantes

    Directory of Open Access Journals (Sweden)

    Mónica Brinquinho

    2017-06-01

    Full Text Available O consumo de esteroides androgénicos anabolizantes tem vindo a aumentar no meio desportivo amador, sendo de fácil acesso apesar de se tratarem de substâncias proibidas. Estão descritos múltiplos efeitos laterais, sendo a hepatotoxicidade uma das mais reportadas. Apresentam-se dois casos clínicos representativos da lesão hepática associada ao consumo de esteroides anabolizantes. Ambos evidenciaram morbilidade significativa, embora nenhum deles tenha evoluído para falência hepática. Os autores pretendem chamar a atenção para o consumo destes fármacos, particularmente frequente em indivíduos jovens, praticantes de desporto amador.

  12. Transcriptome sequencing of the blind subterranean mole rat, Spalax galili: Utility and potential for the discovery of novel evolutionary patterns

    KAUST Repository

    Malik, Assaf

    2011-08-12

    The blind subterranean mole rat (Spalax ehrenbergi superspecies) is a model animal for survival under extreme environments due to its ability to live in underground habitats under severe hypoxic stress and darkness. Here we report the transcriptome sequencing of Spalax galili, a chromosomal type of S. ehrenbergi. cDNA pools from muscle and brain tissues isolated from animals exposed to hypoxic and normoxic conditions were sequenced using Sanger, GS FLX, and GS FLX Titanium technologies. Assembly of the sequences yielded over 51,000 isotigs with homology to ~12,000 mouse, rat or human genes. Based on these results, it was possible to detect large numbers of splice variants, SNPs, and novel transcribed regions. In addition, multiple differential expression patterns were detected between tissues and treatments. The results presented here will serve as a valuable resource for future studies aimed at identifying genes and gene regions evolved during the adaptive radiation associated with underground life of the blind mole rat. 2011 Malik et al.

  13. Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia from South-Eastern Europe

    Directory of Open Access Journals (Sweden)

    Ivo Savić

    2017-10-01

    Full Text Available Mole rats are exclusively subterranean and highly specialized rodents. Their long lifespans, remarkable anti-cancer mechanisms, and various distinctive adaptive features make them a useful research model. Moreover, opposing convergence of morphological traits, they have developed extremely high karyotype variability. Thus, 74 chromosomal forms have been described so far and new ones are being revealed continuously. These evolved during the process of rapid radiation and occur in different biogeographical regions. During research into their reproductive biology we have already provided substantial evidence for species-level separation of these taxa. Here, we review diverse chromosomal forms of the lesser blind mole rat, Mediterranean Nannospalax leucodon, distributed in South-eastern Europe, their karyotype records, biogeography, origin, and phylogeny from our extensive research. In the light of new data from molecular genetic studies, we question some former valuations and propose a cryptospecies rank for seven reproductively isolated chromosomal forms with sympatric and parapatric distribution and clear ecogeographical discrepances in their habitats, as well as new experimental and theoretical methods for understanding the courses of speciation of these unique fossorial mammals.

  14. Environmental physiology of the mole crab Emerita asiatica, at a power plant discharge area on the east coast of India

    International Nuclear Information System (INIS)

    Suresh, K.; Ahamed, M.S.; Durairaj, G.; Nair, K.V.K.

    1995-01-01

    Cage experiments at the discharge area of Madras Atomic Power Station (MAPS) facilitated studies of thermal tolerance in Emerita asiatica. At the laboratory, oxygen consumption at various temperatures and varying salinities was also investigated. In the field 100% mortality of crabs was recorded at the Condenser Cooling Water Pumps (CCWP) discharge site compared to no mortality at the Processed Sea Water Pumps (PSWP) site. This observation implicated temperature as a stress factor at the CCWP outfall, because other factors, including residual chlorine and water velocity, were the same at the PSWP and CCWP sites. Laboratory experiments on tolerance revealed that 38.5 o C was lethal to mole crabs. The time taken for 100% mortality decreased as the temperature increased from 35 to 40 o C. Oxygen metabolism showed a progressive increase with temperature from 29 to 36 o c, and declined at 37 o C. The influence of salinity on oxygen consumption was marginal at salinities of 20 to 35o/oo but, when reduced to 15o/oo, the oxygen consumption declined. The present study thus indicates that temperature could be the lethal factor, determining the distribution of mole crabs near the power station, where water temperature can exceed 40 o C. (author)

  15. The anti-tumorigenic activity of A2M-A lesson from the naked mole-rat.

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    Susanne Kurz

    Full Text Available Cancer resistance is a major cause for longevity of the naked mole-rat. Recent liver transcriptome analysis in this animal compared to wild-derived mice revealed higher expression of alpha2-macroglobulin (A2M and cell adhesion molecules, which contribute to the naked mole-rat's cancer resistance. Notably, A2M is known to dramatically decrease with age in humans. We hypothesize that this might facilitate tumour development. Here we found that A2M modulates tumour cell adhesion, migration and growth by inhibition of tumour promoting signalling pathways, e.g. PI3K / AKT, SMAD and up-regulated PTEN via down-regulation of miR-21, in vitro and in tumour xenografts. A2M increases the expression of CD29 and CD44 but did not evoke EMT. Transcriptome analysis of A2M-treated tumour cells, xenografts and mouse liver demonstrated a multifaceted regulation of tumour promoting signalling pathways indicating a less tumorigenic environment mediated by A2M. By virtue of these multiple actions the naturally occurring A2M has strong potential as a novel therapeutic agent.

  16. The anti-tumorigenic activity of A2M-A lesson from the naked mole-rat.

    Science.gov (United States)

    Kurz, Susanne; Thieme, René; Amberg, Ronny; Groth, Marco; Jahnke, Heinz-Georg; Pieroh, Philipp; Horn, Lars-Christian; Kolb, Marlen; Huse, Klaus; Platzer, Matthias; Volke, Daniela; Dehghani, Faramarz; Buzdin, Anton; Engel, Kathrin; Robitzki, Andrea; Hoffmann, Ralf; Gockel, Ines; Birkenmeier, Gerd

    2017-01-01

    Cancer resistance is a major cause for longevity of the naked mole-rat. Recent liver transcriptome analysis in this animal compared to wild-derived mice revealed higher expression of alpha2-macroglobulin (A2M) and cell adhesion molecules, which contribute to the naked mole-rat's cancer resistance. Notably, A2M is known to dramatically decrease with age in humans. We hypothesize that this might facilitate tumour development. Here we found that A2M modulates tumour cell adhesion, migration and growth by inhibition of tumour promoting signalling pathways, e.g. PI3K / AKT, SMAD and up-regulated PTEN via down-regulation of miR-21, in vitro and in tumour xenografts. A2M increases the expression of CD29 and CD44 but did not evoke EMT. Transcriptome analysis of A2M-treated tumour cells, xenografts and mouse liver demonstrated a multifaceted regulation of tumour promoting signalling pathways indicating a less tumorigenic environment mediated by A2M. By virtue of these multiple actions the naturally occurring A2M has strong potential as a novel therapeutic agent.

  17. Negligible senescence in the longest living rodent, the naked mole-rat: insights from a successfully aging species.

    Science.gov (United States)

    Buffenstein, Rochelle

    2008-05-01

    Aging refers to a gradual deterioration in function that, over time, leads to increased mortality risk, and declining fertility. This pervasive process occurs in almost all organisms, although some long-lived trees and cold water inhabitants reportedly show insignificant aging. Negligible senescence is characterized by attenuated age-related change in reproductive and physiological functions, as well as no observable age-related gradual increase in mortality rate. It was questioned whether the longest living rodent, the naked mole-rat, met these three strict criteria. Naked mole-rats live in captivity for more than 28.3 years, approximately 9 times longer than similar-sized mice. They maintain body composition from 2 to 24 years, and show only slight age-related changes in all physiological and morphological characteristics studied to date. Surprisingly breeding females show no decline in fertility even when well into their third decade of life. Moreover, these animals have never been observed to develop any spontaneous neoplasm. As such they do not show the typical age-associated acceleration in mortality risk that characterizes every other known mammalian species and may therefore be the first reported mammal showing negligible senescence over the majority of their long lifespan. Clearly physiological and biochemical processes in this species have evolved to dramatically extend healthy lifespan. The challenge that lies ahead is to understand what these mechanisms are.

  18. Walking the oxidative stress tightrope: a perspective from the naked mole-rat, the longest-living rodent.

    Science.gov (United States)

    Rodriguez, Karl A; Wywial, Ewa; Perez, Viviana I; Lambert, Adriant J; Edrey, Yael H; Lewis, Kaitlyn N; Grimes, Kelly; Lindsey, Merry L; Brand, Martin D; Buffenstein, Rochelle

    2011-01-01

    Reactive oxygen species (ROS), by-products of aerobic metabolism, cause oxidative damage to cells and tissue and not surprisingly many theories have arisen to link ROS-induced oxidative stress to aging and health. While studies clearly link ROS to a plethora of divergent diseases, their role in aging is still debatable. Genetic knock-down manipulations of antioxidants alter the levels of accrued oxidative damage, however, the resultant effect of increased oxidative stress on lifespan are equivocal. Similarly the impact of elevating antioxidant levels through transgenic manipulations yield inconsistent effects on longevity. Furthermore, comparative data from a wide range of endotherms with disparate longevity remain inconclusive. Many long-living species such as birds, bats and mole-rats exhibit high-levels of oxidative damage, evident already at young ages. Clearly, neither the amount of ROS per se nor the sensitivity in neutralizing ROS are as important as whether or not the accrued oxidative stress leads to oxidative-damage-linked age-associated diseases. In this review we examine the literature on ROS, its relation to disease and the lessons gleaned from a comparative approach based upon species with widely divergent responses. We specifically focus on the longest lived rodent, the naked mole-rat, which maintains good health and provides novel insights into the paradox of maintaining both an extended healthspan and lifespan despite high oxidative stress from a young age.

  19. Disparate patterns of age-related changes in lipid peroxidation in long-lived naked mole-rats and shorter-lived mice.

    Science.gov (United States)

    Andziak, Blazej; Buffenstein, Rochelle

    2006-12-01

    A key tenet of the oxidative stress theory of aging is that levels of accrued oxidative damage increase with age. Differences in damage generation and accumulation therefore may underlie the natural variation in species longevity. We compared age-related profiles of whole-organism lipid peroxidation (urinary isoprostanes) and liver lipid damage (malondialdehyde) in long living naked mole-rats [maximum lifespan (MLS) > 28.3 years] and shorter-living CB6F1 hybrid mice (MLS approximately 3.5 years). In addition, we compared age-associated changes in liver non-heme iron to assess how intracellular conditions, which may modulate oxidative processes, are affected by aging. Surprisingly, even at a young age, concentrations of both markers of lipid peroxidation, as well as of iron, were at least twofold (P naked mole tats than in mice. This refutes the hypothesis that prolonged naked mole-rat longevity is due to superior protection against oxidative stress. The age-related profiles of all three parameters were distinctly species specific. Rates of lipid damage generation in mice were maintained throughout adulthood, while accrued damage in old animals was twice that of young mice. In naked mole-rats, urinary isoprostane excretion declined by half with age (P naked mole-rats is independent of oxidative stress parameters.

  20. Fos expression in the suprachiasmatic nucleus in response to light stimulation in a solitary and social species of African mole-rat (family Bathyergidae).

    Science.gov (United States)

    Oosthuizen, M K; Bennett, N C; Cooper, H M

    2005-01-01

    Mole-rats are strictly subterranean rodents that are rarely exposed to environmental light. They are well adapted to their environment and have reduced eyes and a severely regressed visual system. It has been shown, however, that mole-rats do exhibit endogenous circadian rhythms that can be entrained, suggesting an intact and functional circadian system. To determine whether light is the entraining agent in these animals, Fos expression in response to light pulses at different circadian times was investigated to obtain phase response curves. Light is integrated effectively in the suprachiasmatic nucleus of the Cape mole-rat (Georychus capensis), and Fos expression is gated according to the phase of the circadian clock. The Fos response in the Cape mole-rat was comparable to that of aboveground rodents. In contrast, the highveld mole-rat (Cryptomys hottentotus pretoriae) was less sensitive to light and did not show a selective Fos response according to the phase of the circadian cycle. Social species appear to be less sensitive to light than their solitary counterparts, which compares well with results from locomotor activity studies.

  1. Photon counting arrays for AO wavefront sensors

    CERN Document Server

    Vallerga, J; McPhate, J; Mikulec, Bettina; Clark, Allan G; Siegmund, O; CERN. Geneva

    2005-01-01

    Future wavefront sensors for AO on large telescopes will require a large number of pixels and must operate at high frame rates. Unfortunately for CCDs, there is a readout noise penalty for operating faster, and this noise can add up rather quickly when considering the number of pixels required for the extended shape of a sodium laser guide star observed with a large telescope. Imaging photon counting detectors have zero readout noise and many pixels, but have suffered in the past with low QE at the longer wavelengths (>500 nm). Recent developments in GaAs photocathode technology, CMOS ASIC readouts and FPGA processing electronics have resulted in noiseless WFS detector designs that are competitive with silicon array detectors, though at ~40% the QE of CCDs. We review noiseless array detectors and compare their centroiding performance with CCDs using the best available characteristics of each. We show that for sub-aperture binning of 6x6 and greater that noiseless detectors have a smaller centroid error at flu...

  2. Adesao ao guia alimentar para populacao brasileira

    Directory of Open Access Journals (Sweden)

    Eliseu Verly Junior

    2013-12-01

    Full Text Available OBJETIVO : Analisar a adesão ao Guia Alimentar para População Brasileira. MÉTODOS : Amostra composta por participantes do Inquérito de Saúde de São Paulo (n = 1.661 que preencheram dois recordatórios de 24 horas. Foi utilizado modelo bivariado de efeito misto para a razão entre o consumo de energia do grupo de alimentos e o consumo calórico total. A razão estimada foi utilizada para calcular o percentual de indivíduos com consumo abaixo ou acima da recomendação. RESULTADOS : Pelo menos 80,0% da população consome abaixo do recomendado para: leite e derivados; frutas e sucos de frutas; e cereais, tubérculos e raízes; aproximadamente 60,0% para legumes e verduras; 30,0% para feijões; e 8,0% para carnes e ovos. Adolescentes apresentaram a maior inadequação para legumes e verduras (90,0%, e o estrato de maior renda foi associado à menor inadequação para óleos, gorduras e sementes oleaginosas (57,0%. CONCLUSÕES : Foi observado consumo inadequado dos grupos de alimentos relacionados com aumento do risco de doenças crônicas.

  3. Toxicologia do tolueno: aspectos relacionados ao abuso

    Directory of Open Access Journals (Sweden)

    Forster Letícia M.K.

    1994-01-01

    Full Text Available O tolueno está presente em muitos produtos de uso doméstico e industrial e é o principal solvente envolvido no abuso de substâncias e na exposição ocupacional. O problema mais grave no estudo de patologias relacionadas ao tolueno é que este está geralmente associado, em suas preparações comerciais, a outras substâncias. O potencial tóxico do tolueno foi abordado nos seguintes aspectos: parâmetros farmacológicos; características físico-químicas; exposição; estudos clínicos; diagnóstico; pesquisa experimental; tolerância e dependência; efeitos agudos e crônicos; neurotoxicidade; teratogenicidade; doenças psiquiátricas; carcinogenicidade e tratamento. Conclui-se ser de grande importância e urgência que se realizem estudos clínicos com amostras maiores para definição mais precisa das conseqüências do uso crônico

  4. Toxicologia do tolueno: aspectos relacionados ao abuso

    Directory of Open Access Journals (Sweden)

    Letícia M.K. Forster

    1994-04-01

    Full Text Available O tolueno está presente em muitos produtos de uso doméstico e industrial e é o principal solvente envolvido no abuso de substâncias e na exposição ocupacional. O problema mais grave no estudo de patologias relacionadas ao tolueno é que este está geralmente associado, em suas preparações comerciais, a outras substâncias. O potencial tóxico do tolueno foi abordado nos seguintes aspectos: parâmetros farmacológicos; características físico-químicas; exposição; estudos clínicos; diagnóstico; pesquisa experimental; tolerância e dependência; efeitos agudos e crônicos; neurotoxicidade; teratogenicidade; doenças psiquiátricas; carcinogenicidade e tratamento. Conclui-se ser de grande importância e urgência que se realizem estudos clínicos com amostras maiores para definição mais precisa das conseqüências do uso crônico

  5. Clinical significance of measurement of changes of serum TNF-α and EGF levels after chemotherapy in patients with malignant hydatidiform mole

    International Nuclear Information System (INIS)

    Chen Xiaochao; Zhou Dongxia; Zhang Liming

    2008-01-01

    Objective: To study the clinical significance of changes of serum TNF-α and EGF levels after chemotherapy in patients with malignant hydatidiform mole. Methods: Serum TNF-α, EGF levels (with RIA) were measured both before and after chemotherapy in 32 patients with malignant hydatidiform mole as well as in 35 controls. Results: Before chemotherapy, serum TNF- α and EGF levels were significantly higher in the patients than those in controls (P<0.01). Six months after chemotherapy, serum TNF-α and EGF levels, though dropped markedly, remained significantly higher than those in controls (P<0.05). Conclusion: Development of malignant hydatidiform mole in patients was closely related to the serum TNF-α and EGF levels. (authors)

  6. The naked mole-rat exhibits an unusual cardiac myofilament protein profile providing new insights into heart function of this naturally subterranean rodent.

    Science.gov (United States)

    Grimes, Kelly M; Barefield, David Y; Kumar, Mohit; McNamara, James W; Weintraub, Susan T; de Tombe, Pieter P; Sadayappan, Sakthivel; Buffenstein, Rochelle

    2017-12-01

    The long-lived, hypoxic-tolerant naked mole-rat well-maintains cardiac function over its three-decade-long lifespan and exhibits many cardiac features atypical of similar-sized laboratory rodents. For example, they exhibit low heart rates and resting cardiac contractility, yet have a large cardiac reserve. These traits are considered ecophysiological adaptations to their dank subterranean atmosphere of low oxygen and high carbon dioxide levels and may also contribute to negligible declines in cardiac function during aging. We asked if naked mole-rats had a different myofilament protein signature to that of similar-sized mice that commonly show both high heart rates and high basal cardiac contractility. Adult mouse ventricles predominantly expressed α-myosin heavy chain (97.9 ± 0.4%). In contrast, and more in keeping with humans, β myosin heavy chain was the dominant isoform (79.0 ± 2.0%) in naked mole-rat ventricles. Naked mole-rat ventricles diverged from those of both humans and mice, as they expressed both cardiac and slow skeletal isoforms of troponin I. This myofilament protein profile is more commonly observed in mice in utero and during cardiomyopathies. There were no species differences in phosphorylation of cardiac myosin binding protein-C or troponin I. Phosphorylation of both ventricular myosin light chain 2 and cardiac troponin T in naked mole-rats was approximately half that observed in mice. Myofilament function was also compared between the two species using permeabilized cardiomyocytes. Together, these data suggest a cardiac myofilament protein signature that may contribute to the naked mole-rat's suite of adaptations to its natural subterranean habitat.

  7. Sensibilidade de estirpes de Bradyrhizobium ao glyphosate

    Directory of Open Access Journals (Sweden)

    Rodrigo Josemar Seminoti Jacques

    2010-02-01

    Full Text Available A aplicação do glyphosate sobre a soja resistente a este herbicida pode causar prejuízos à simbiose com o rizóbio. O objetivo deste trabalho foi avaliar a sensibilidade ao herbicida glyphosate de três estirpes de Bradyrhizobium recomendadas para a produção de inoculantes de sementes de soja no Brasil. Avaliou-se o efeito das concentrações de 0,0; 5,4; 10,8; 21,6 e 43,2 µg L-1 do ingrediente ativo do glyphosate [N-(fosfonometil glicina] no meio YM líquido sobre o crescimento de B. japonicum (estirpe SEMIA 5079 e de B. elkanii (estirpe SEMIA 5019 e estirpe SEMIA 587, por meio de leituras das densidades óticas e geração de curvas de crescimento. As reduções de crescimento na presença da menor concentração do glyphosate foram de 18% para SEMIA 5079, 29% para SEMIA 5019 e de 35% para SEMIA 587, sendo, de modo geral, quanto maior a concentração do herbicida no meio de cultura maior a inibição do crescimen­to. As estirpes apresentaram sensibilidade diferencial somente às concentrações mais baixas do glyphosate; nesse caso, foi possível determinar a seguinte ordem de sensibilidade: SEMIA 587 > SEMIA 5019 > SEMIA 5079. Essa sensibilidade diferencial é dependente da concentração do herbicida, pois na presença de 43,2 µg L-1 todas as estirpes tiveram seu crescimento severamente reduzido, não havendo diferença entre elas.

  8. Familial atypical multiple mole melanoma syndrome in an adult Indian male-case report and literature review

    Directory of Open Access Journals (Sweden)

    Radhika C G Raj

    2015-01-01

    Full Text Available Familial atypical multiple mole melanoma syndrome (FAMMMS is an autosomal dominant genodermatosis characterized by multiple melanocytic nevi, usually more than 50, and a family history of melanoma. It is known to be associated with carcinoma of pancreas and other malignancies involving gastrointestinal tract, breast, lung, larynx, and skin in the kindred. There is no published report of FAMMMS in dark-skinned individuals. We report a case of FAMMMS in a dark-skinned adult Indian male, who had multiple extensive nevi all over the body and oral mucosa; associated with malignant melanoma, squamous cell carcinoma (Marjolin′s ulcer, and carcinoma of pancreas. His father had died of carcinoma of lung and his sister had a partial phenotypic expression. The clinical presentation of the case is discussed with review of literature.

  9. Spatial and temporal activity patterns of the free-living giant mole-rat (Fukomys mechowii, the largest social bathyergid.

    Directory of Open Access Journals (Sweden)

    Matěj Lövy

    Full Text Available Despite the considerable attention devoted to the biology of social species of African mole-rats (Bathyergidae, Rodentia, knowledge is lacking about their behaviour under natural conditions. We studied activity of the largest social bathyergid, the giant mole-rat Fukomys mechowii, in its natural habitat in Zambia using radio-telemetry. We radio-tracked six individuals during three continuous 72-h sessions. Five of these individuals, including a breeding male, belonged to a single family group; the remaining female was probably a solitary disperser. The non-breeders of the family were active (i.e. outside the nest 5.8 hours per 24h-day with the activity split into 6.5 short bouts. The activity was more concentrated in the night hours, when the animals also travelled longer distances from the nest. The breeding male spent only 3.2 hours per day outside the nest, utilizing less than 20% of the whole family home range. The dispersing female displayed a much different activity pattern than the family members. Her 8.0 hours of outside-nest activity per day were split into 4.6 bouts which were twice as long as in the family non-breeders. Her activity peak in the late afternoon coincided with the temperature maximum in the depth of 10 cm (roughly the depth of the foraging tunnels. Our results suggest that the breeding individuals (at least males contribute very little to the work of the family group. Nevertheless, the amount of an individual's activity and its daily pattern are probably flexible in this species and can be modified in response to actual environmental and social conditions.

  10. Response of mole crab, Emerita emeritus to the thermal effluents from the Madras Atomic Power Station at Kalpakkam

    International Nuclear Information System (INIS)

    Subramoniam, T.; Munuswamy, N.; Israel, Sunil; Sathish, R.; Venugopalan, V.P.

    2007-01-01

    Mole crabs belonging to the genus Emerita are exclusively inhabitants of exposed wave washed sandy beaches in certain temperate and tropical seas. Its sedentary adult life and a primitive filter-feeding behavior make this intertidal crab an excellent indicator species for monitoring anthropogenic impact. Hence, this sand crab has been used to determine the effect of elevated temperature (rT) arising out of the thermal effluent from the Madras Atomic Power Station (MAPS), Kalpakkam with special reference to the intertidal fauna. Since Emerita exhibits limited capacity to move from their place of habitation, an analysis of the population structure of Emerita emeritus in the Kalpakkam coast including the impacted and the adjoining control sandy beaches on either side of the mixing point in the vicinity of the power plant was done. The data indicate that in the impact zone having an elevated seawater temperature of <35 deg C, the crab is completely absent. However, as we move away from the impact zone, with normalization of seawater temperature, Emerita move to safer areas on either side of the impact zone. Thus, the extensive field data on the distribution of mole crabs indicated that population structure is affected by the thermal plume, although the shifting positions of the mixing point (caused by northerly and southerly long shore currents) allowed decolonization of Emerita due to transmigration in positions that were earlier mixing points. This paper also describes a complete larval development of Emerita emeritus in the laboratory. Different life stages of E. emeritus were subjected to various power plant stressors to determine their varying responses. The expression of heat shock protein (hsp) in the gill tissue of the adult crabs was also observed when the crabs were exposed to elevated temperatures (35 deg C). However, no expression of heat shock proteins was observed when the crabs were subjected to chlorine stress. (author)

  11. Resistance to experimental tumorigenesis in cells of a long-lived mammal, the naked mole-rat (Heterocephalus glaber).

    Science.gov (United States)

    Liang, Sitai; Mele, James; Wu, Yuehong; Buffenstein, Rochelle; Hornsby, Peter J

    2010-08-01

    The naked mole-rat (NMR, Heterocephalus glaber) is a long-lived mammal in which spontaneous cancer has not been observed. To investigate possible mechanisms for cancer resistance in this species, we studied the properties of skin fibroblasts from the NMR following transduction with oncogenes that cause cells of other mammalian species to form malignant tumors. Naked mole-rat fibroblasts were transduced with a retrovirus encoding SV40 large T antigen and oncogenic Ras(G12V). Following transplantation of transduced cells into immunodeficient mice, cells rapidly entered crisis, as evidenced by the presence of anaphase bridges, giant cells with enlarged nuclei, multinucleated cells, and cells with large number of chromosomes or abnormal chromatin material. In contrast, similarly transduced mouse and rat fibroblasts formed tumors that grew rapidly without crisis. Crisis was also observed after > 40 population doublings in SV40 TAg/Ras-expressing NMR cells in culture. Crisis in culture was prevented by additional infection of the cells with a retrovirus encoding hTERT (telomerase reverse transcriptase). SV40 TAg/Ras/hTERT-expressing NMR cells formed tumors that grew rapidly in immunodeficient mice without evidence of crisis. Crisis could also be induced in SV40 TAg/Ras-expressing NMR cells by loss of anchorage, but after hTERT transduction, cells were able to proliferate normally following loss of anchorage. Thus, rapid crisis is a response of oncogene-expressing NMR cells to growth in an in vivo environment, which requires anchorage independence, and hTERT permits cells to avoid crisis and to achieve malignant tumor growth. The unique reaction of NMR cells to oncogene expression may form part of the cancer resistance of this species.

  12. Metabolic clues to salubrious longevity in the brain of the longest-lived rodent: the naked mole-rat.

    Science.gov (United States)

    Triplett, Judy C; Swomley, Aaron; Kirk, Jessime; Lewis, Katilyn; Orr, Miranda; Rodriguez, Karl; Cai, Jian; Klein, Jon B; Buffenstein, Rochelle; Butterfield, D Allan

    2015-08-01

    Naked mole-rats (NMRs) are the oldest-living rodent species. Living underground in a thermally stable ecological niche, NMRs have evolved certain exceptional traits, resulting in sustained health spans, negligible cognitive decline, and a pronounced resistance to age-related disease. Uncovering insights into mechanisms underlying these extraordinary traits involved in successful aging may conceivably provide crucial clues to extend the human life span and health span. One of the most fundamental processes inside the cell is the production of ATP, which is an essential fuel in driving all other energy-requiring cellular activities. Not surprisingly, a prominent hallmark in age-related diseases, such as neurodegeneration and cancer, is the impairment and dysregulation of metabolic pathways. Using a two-dimensional polyacrylamide gel electrophoresis proteomics approach, alterations in expression and phosphorylation levels of metabolic proteins in the brains of NMRs, aged 2-24 years, were evaluated in an age-dependent manner. We identified 13 proteins with altered levels and/or phosphorylation states that play key roles in various metabolic pathways including glycolysis, β-oxidation, the malate-aspartate shuttle, the Tricarboxylic Acid Cycle (TCA) cycle, the electron transport chain, NADPH production, as well as the production of glutamate. New insights into potential pathways involved in metabolic aspects of successful aging have been obtained by the identification of key proteins through which the NMR brain responds and adapts to the aging process and how the NMR brain adapted to resist age-related degeneration. This study examines the changes in the proteome and phosphoproteome in the brain of the naked mole-rat aged 2-24 years. We identified 13 proteins (labeled in red) with altered expression and/or phosphorylation levels that are conceivably associated with sustained metabolic functions in the oldest NMRs that may promote a sustained health span and life span.

  13. Subcaste differences in neural activation suggest a prosocial role for oxytocin in eusocial naked mole-rats.

    Science.gov (United States)

    Hathaway, Georgia A; Faykoo-Martinez, Mariela; Peragine, Deane E; Mooney, Skyler J; Holmes, Melissa M

    2016-03-01

    The neuropeptide oxytocin (OT) influences prosocial behavior(s), aggression, and stress responsiveness, and these diverse effects are regulated in a species- and context-specific manner. The naked mole-rat (Heterocephalus glaber) is a unique species with which to study context-dependent effects of OT, exhibiting a strict social hierarchy with behavioral specialization within the subordinate caste: soldiers are aggressive and defend colonies against unfamiliar conspecifics while workers are prosocial and contribute to in-colony behaviors such as pup care. To determine if OT is involved in subcaste-specific behaviors, we compared behavioral responses between workers and soldiers of both sexes during a modified resident/intruder paradigm, and quantified activation of OT neurons in the hypothalamic paraventricular nucleus (PVN) and supraoptic nucleus (SON) using the immediate-early-gene marker c-fos co-localized with OT neurons. Resident workers and soldiers were age-matched with unfamiliar worker stimulus animals as intruders, and encounters were videorecorded and scored for aggressive behaviors. Colony-matched controls were left in their home colony for the duration of the encounters. Brains were extracted and cell counts were conducted for OT immunoreactive (ir), c-fos-ir, and percentage of OT-c-fos double-labeled cells. Results indicate that resident workers were less aggressive but showed greater OT neural activity than soldiers. Furthermore, a linear model including social treatment, cortisol, and subcaste revealed that subcaste was the only significant predictor of OT-c-fos double-labeled cells in the PVN. These data suggest that in naked mole-rats OT promotes prosocial behaviors rather than aggression and that even within subordinates status exerts robust effects on brain and behavior. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Análise do perfil facial esquelético e de tecidos moles pré e pós-cirurgia ortognática em pacientes Classe II e III, e sua relação com a proporção áurea

    Directory of Open Access Journals (Sweden)

    Leticia Ângelo WALEWSKI

    Full Text Available Resumo Introdução A proporção áurea, representada pela razão matemática 1:1.618, tem sido investigada na especialidade Ortodontia e no procedimento de Cirurgia Ortognática, a fim de ser estabelecida como um guia de sucesso clínico relacionado à estética facial. Objetivo Verificar a estética dos perfis faciais de pacientes Classes II e III de Angle, antes e após tratamento ortodôntico-cirúrgico, além de correlacionar 13 razões dentoesqueléticas e cinco razões de tecidos moles à proporção áurea. Material e método Um total de 94 radiografias cefalométricas laterais foram analisadas, em que 13 razões dentoesqueléticas e cinco razões em tecidos moles foram medidas e comparadas ao número áureo. Adicionalmente, uma análise subjetiva da estética facial pré e pós-tratamento foi realizada por 270 examinadores. Resultado As razões dentoesqueléticas 1, 3, 6, 7, 8 e 9 aproximaram-se do número áureo após a cirurgia ortognática, nos pacientes Classe III. Para o perfil mole, apenas a razão 4 se aproximou ao número áureo, em ambos os pacientes Classe II e III. Em relação à avaliação subjetiva da estética, 91,49% dos perfis faciais foram considerados mais harmônicos após o tratamento. Conclusão Frente à metodologia usada, pode-se concluir que a proporção áurea exerce pouca influência na avaliação da estética facial, não servindo como um guia para o planejamento e o tratamento ortodôntico-ortognático.

  15. CARACTERÍSTICAS ESTRUTURAIS DA MADEIRA DE Pinus elliottii Engelm AOS 30 ANOS DE IDADE

    Directory of Open Access Journals (Sweden)

    Clovis Roberto Haselein

    2000-09-01

    Full Text Available No presente estudo, seis árvores de um povoamento de Pinus elliottii com 30 anos de idade foram coletadas e transformadas em toras de 2,5 m de comprimento. De cada uma das duas primeiras toras, retirou-se um pranchão central, do qual foram confeccionados, segundo a norma ASTM D143-93 (1995, nas regiões próximas à medula e à casca, os corpos de prova utilizados nos ensaios de flexão estática. As propriedades de flexão (módulos de elasticidade e de ruptura e massa específica básica a 12% de umidade foram relacionados como número de anéis de crescimento e a porcentagem de lenho tardio determinados na secção transversal para cada corpo de prova. Os resultados evidenciaram, para as propriedades estudadas, a existência de diferenças significativas entre as posições.

  16. Adaptabilidade de trigo mole às condições mediterrânicas em função do seu ciclo vegetativo Adaptability of bread wheat under mediterranean conditions as influenced by its vegetative cycle

    Directory of Open Access Journals (Sweden)

    José Manuel Godinho Calado

    2008-01-01

    Full Text Available O estudo realizado tinha como objectivo verificar a capacidade de adaptação de genótipos de trigo mole, quantificada pela resposta produtiva, às condições mediterrânicas em função do seu ciclo vegetativo. Devido à irregularidade das chuvas de Outono, que afecta a data óptima de sementeira, é de extrema importância para a cerealicultura das regiões mediterrâneas. Na experimentação efectuaram-se diferentes datas de sementeira de quinze genótipos de trigo mole durante cinco anos. O delineamento experimental foi em blocos casualizados com divisão dos talhões e quatro repetições. A partir dos resultados, conclui-se que o ciclo vegetativo do trigo mole de 125 a 145 dias desde a sementeira até ao espigamento ou de 1350 a 1450 ºC permite uma boa adaptação dos genótipos às condições mediterrânicas e, em consequência, uma boa resposta produtiva. Assim, as variedades que apresentam um ciclo vegetativo com a duração referida, mesmo quando a data de sementeira varia entre o fim de Outubro e o fim de Novembro, como é o exemplo da variedade Nabão, adaptam-se à irregularidade das condições mediterrânicas e atingem rendimentos aceitáveis.This study was conducted to verify the adaptability of bread wheat genotypes with different vegetative cycles to Mediterranean conditions in terms of their productivity. Due to the very irregular rainfall in autumn, which conditions sowing date, the knowledge of the performance of the wheat varieties used with regard to different sowing dates is of extreme importance for a successful cereal production under Mediterranean conditions. Fifteen wheat bread genotypes were sown at different dates during autumn over a period of five years. The experimental layout was a randomized block design for sowing dates with a split for the different genotypes and four replications. The results indicate that wheat genotypes with a cycle duration of 125 to 145 days or 1350 to 1450 days • degree (d

  17. Parentage analysis of Ansell’s mole-rat family groups indicates a high reproductive skew despite relatively relaxed ecological constraints on dispersal

    Czech Academy of Sciences Publication Activity Database

    Patzenhauerová, Hana; Šklíba, J.; Bryja, Josef; Šumbera, R.

    2013-01-01

    Roč. 22, č. 19 (2013), s. 4988-5000 ISSN 0962-1083 R&D Projects: GA AV ČR IAA601410802; GA ČR GAP506/10/0983 Institutional support: RVO:68081766 Keywords : African mole-rat * dispersal * eusociality * Fukomys * mating system * reproductive skew Subject RIV: EH - Ecology, Behaviour Impact factor: 5.840, year: 2013

  18. The Evasion of the Text; Invasion of the Text; Again, Evasion of the Text; "The Eagle and the Mole": The Affective Fallacy Revisited.

    Science.gov (United States)

    Ducharme, Edward R.; And Others

    1970-01-01

    Three writers debate whether English teachers should depend on external or internal criteria when analyzing and teaching literature. Each writer discusses the meaning of Elinor Wylie's poem, "The Eagle and the Mole," according to his particular view of literary analysis. Edward Ducharme, in "The Evasion of the Text," submits…

  19. Variability of space-use patterns in a free living eusocial rodent, Ansell’s mole-rat indicates age-based rather than caste polyethism

    Czech Academy of Sciences Publication Activity Database

    Šklíba, Jan; Lövy, M.; Burda, H.; Šumbera, R.

    2016-01-01

    Roč. 6, DEC 06 (2016), č. článku 37497. ISSN 2045-2322 EU Projects: European Commission(XE) 669609 - Diversity6continents Institutional support: RVO:60077344 Keywords : eusocial rodent * mole-rats * Fukomys anselli Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 4.259, year: 2016 https://www.nature.com/articles/srep37497

  20. An energy stable evolution method for simulating two-phase equilibria of multi-component fluids at constant moles, volume and temperature

    KAUST Repository

    Kou, Jisheng; Sun, Shuyu; Wang, Xiuhua

    2016-01-01

    In this paper, we propose an energy-stable evolution method for the calculation of the phase equilibria under given volume, temperature, and moles (VT-flash). An evolution model for describing the dynamics of two-phase fluid system is based on Fick

  1. Identification of patients with persistent trophoblastic disease after complete hydatidiform mole by using a normal 24-hour urine hCG regression curve

    NARCIS (Netherlands)

    Cromvoirt, S.M. van; Thomas, C.M.G.; Quinn, M.A.; McNally, O.M.; Bekkers, R.L.M.

    2014-01-01

    OBJECTIVE: The aim of this study was to establish a reference 24-hour urine human chorionic gonadotropin (hCG) regression curve in patients with complete hydatidiform mole (CHM) as diagnostic tool in the prediction of persistent trophoblastic disease (PTD). METHODS: From 2004 to 2011, 312 cases

  2. SCExAO: First Results and On-Sky Performance

    Science.gov (United States)

    Currie, Thayne; Guyon, Olivier; Martinache, Frantz; Clergeon, Christophe; McElwain, Michael; Thalmann, Christian; Jovanovic, Nemanja; Singh, Garima; Kudo, Tomoyuki

    2013-01-01

    We present new on-sky results for the Subaru Coronagraphic Extreme Adaptive Optics imager (SCExAO) verifying and quantifying the contrast gain enabled by key components: the closed-loop coronagraphic low-order wavefront sensor (CLOWFS) and focal plane wavefront control ("speckle nulling"). SCExAO will soon be coupled with a high-order, Pyramid wavefront sensor which will yield greater than 90% Strehl ratio and enable 10(exp 6) -10(exp 7) contrast at small angular separations allowing us to image gas giant planets at solar system scales. Upcoming instruments like VAMPIRES, FIRST, and CHARIS will expand SCExAO's science capabilities.

  3. Effects of laboratory housing on exploratory behaviour, novelty discrimination and spatial reference memory in a subterranean, solitary rodent, the Cape mole-rat (Georychus capensis).

    Science.gov (United States)

    Oosthuizen, Maria Kathleen; Scheibler, Anne-Gita; Bennett, Nigel Charles; Amrein, Irmgard

    2013-01-01

    A large number of laboratory and field based studies are being carried out on mole-rats, both in our research group and others. Several studies have highlighted the development of adverse behaviours in laboratory animals and have emphasised the importance of enrichment for captive animals. Hence we were interested in evaluating how laboratory housing would affect behavioural performance in mole-rats. We investigated exploratory behaviour, the ability to discriminate between novel and familiar environments and reference memory in the solitary Cape mole-rat (Georychus capensis). Our data showed that both wild and captive animals readily explore open spaces and tunnels. Wild animals were however more active than their captive counterparts. In the Y maze two trial discrimination task, wild animals failed to discriminate between novel and familiar environments, while laboratory housed mole-rats showed preferential spatial discrimination in terms of the length of time spent in the novel arm. The performance of the laboratory and wild animals were similar when tested for reference memory in the Y maze, both groups showed a significant improvement compared to the first day, from the 3rd day onwards. Wild animals made more mistakes whereas laboratory animals were slower in completing the task. The difference in performance between wild and laboratory animals in the Y-maze may be as a result of the lower activity of the laboratory animals. Laboratory maintained Cape mole-rats show classic behaviours resulting from a lack of stimulation such as reduced activity and increased aggression. However, they do display an improved novelty discrimination compared to the wild animals. Slower locomotion rate of the laboratory animals may increase the integration time of stimuli, hence result in a more thorough inspection of the surroundings. Unlike the captive animals, wild animals show flexibility in their responses to unpredictable events, which is an important requirement under

  4. Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat.

    Science.gov (United States)

    Rodriguez, Karl A; Edrey, Yael H; Osmulski, Pawel; Gaczynska, Maria; Buffenstein, Rochelle

    2012-01-01

    The longest-lived rodent, the naked mole-rat (Bathyergidae; Heterocephalus glaber), maintains robust health for at least 75% of its 32 year lifespan, suggesting that the decline in genomic integrity or protein homeostasis routinely observed during aging, is either attenuated or delayed in this extraordinarily long-lived species. The ubiquitin proteasome system (UPS) plays an integral role in protein homeostasis by degrading oxidatively-damaged and misfolded proteins. In this study, we examined proteasome activity in naked mole-rats and mice in whole liver lysates as well as three subcellular fractions to probe the mechanisms behind the apparently enhanced effectiveness of UPS. We found that when compared with mouse samples, naked mole-rats had significantly higher chymotrypsin-like (ChT-L) activity and a two-fold increase in trypsin-like (T-L) in both whole lysates as well as cytosolic fractions. Native gel electrophoresis of the whole tissue lysates showed that the 20S proteasome was more active in the longer-lived species and that 26S proteasome was both more active and more populous. Western blot analyses revealed that both 19S subunits and immunoproteasome catalytic subunits are present in greater amounts in the naked mole-rat suggesting that the observed higher specific activity may be due to the greater proportion of immunoproteasomes in livers of healthy young adults. It thus appears that proteasomes in this species are primed for the efficient removal of stress-damaged proteins. Further characterization of the naked mole-rat proteasome and its regulation could lead to important insights on how the cells in these animals handle increased stress and protein damage to maintain a longer health in their tissues and ultimately a longer life.

  5. Specific paucity of unmyelinated C-fibers in cutaneous peripheral nerves of the African naked-mole rat: comparative analysis using six species of Bathyergidae.

    Science.gov (United States)

    St John Smith, Ewan; Purfürst, Bettina; Grigoryan, Tamara; Park, Thomas J; Bennett, Nigel C; Lewin, Gary R

    2012-08-15

    In mammalian peripheral nerves, unmyelinated C-fibers usually outnumber myelinated A-fibers. By using transmission electron microscopy, we recently showed that the saphenous nerve of the naked mole-rat (Heterocephalus glaber) has a C-fiber deficit manifested as a substantially lower C:A-fiber ratio compared with other mammals. Here we determined the uniqueness of this C-fiber deficit by performing a quantitative anatomical analysis of several peripheral nerves in five further members of the Bathyergidae mole-rat family: silvery (Heliophobius argenteocinereus), giant (Fukomys mechowii), Damaraland (Fukomys damarensis), Mashona (Fukomys darlingi), and Natal (Cryptomys hottentotus natalensis) mole-rats. In the largely cutaneous saphenous and sural nerves, the naked mole-rat had the lowest C:A-fiber ratio (∼1.5:1 compared with ∼3:1), whereas, in nerves innervating both skin and muscle (common peroneal and tibial) or just muscle (lateral/medial gastrocnemius), this pattern was mostly absent. We asked whether lack of hair follicles alone accounts for the C-fiber paucity by using as a model a mouse that loses virtually all its hair as a consequence of conditional deletion of the β-catenin gene in the skin. These β-catenin loss-of function mice (β-cat LOF mice) displayed only a mild decrease in C:A-fiber ratio compared with wild-type mice (4.42 compared with 3.81). We suggest that the selective cutaneous C-fiber deficit in the cutaneous nerves of naked mole-rats is unlikely to be due primarily to lack of skin hair follicles. Possible mechanisms contributing to this unique peripheral nerve anatomy are discussed. Copyright © 2012 Wiley Periodicals, Inc.

  6. Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat.

    Directory of Open Access Journals (Sweden)

    Karl A Rodriguez

    Full Text Available The longest-lived rodent, the naked mole-rat (Bathyergidae; Heterocephalus glaber, maintains robust health for at least 75% of its 32 year lifespan, suggesting that the decline in genomic integrity or protein homeostasis routinely observed during aging, is either attenuated or delayed in this extraordinarily long-lived species. The ubiquitin proteasome system (UPS plays an integral role in protein homeostasis by degrading oxidatively-damaged and misfolded proteins. In this study, we examined proteasome activity in naked mole-rats and mice in whole liver lysates as well as three subcellular fractions to probe the mechanisms behind the apparently enhanced effectiveness of UPS. We found that when compared with mouse samples, naked mole-rats had significantly higher chymotrypsin-like (ChT-L activity and a two-fold increase in trypsin-like (T-L in both whole lysates as well as cytosolic fractions. Native gel electrophoresis of the whole tissue lysates showed that the 20S proteasome was more active in the longer-lived species and that 26S proteasome was both more active and more populous. Western blot analyses revealed that both 19S subunits and immunoproteasome catalytic subunits are present in greater amounts in the naked mole-rat suggesting that the observed higher specific activity may be due to the greater proportion of immunoproteasomes in livers of healthy young adults. It thus appears that proteasomes in this species are primed for the efficient removal of stress-damaged proteins. Further characterization of the naked mole-rat proteasome and its regulation could lead to important insights on how the cells in these animals handle increased stress and protein damage to maintain a longer health in their tissues and ultimately a longer life.

  7. DA PSICODINÂMICA DO TRABALHO AO DIREITO FUNDAMENTAL AO CONTEÚDO DO PRÓPRIO TRABALHO E AO MEIO AMBIENTE ORGANIZACIONAL SAUDÁVEL

    Directory of Open Access Journals (Sweden)

    Leonardo Vieira Wandelli

    2015-10-01

    Full Text Available O presente artigo procura traçar alguns elementos que pretendem articular como, a partir do discurso jurídico, podem ser significados os aportes sobre saúde e trabalho, especialmente aqueles trazidos pela abordagem da Psicodinâmica do Trabalho. Um caminho, assim, que vai dos estudos sobre sujeito, trabalho e saúde até o Direito Constitucional, que afirma como seu fundamento a dignidade humana, indissociável da centralidade antropológica do trabalho; um vínculo expressado pelo direito fundamental ao trabalho que, aqui, se propõe reconstruir a partir do desvelamento de um direito fundamental ao conteúdo do próprio trabalho, articulado ao conceito de meio ambiente organizacional do trabalho. 

  8. [Effects of prophylactic chemotherapy on outcomes and prognosis of patients older than 40 years with invasive mole].

    Science.gov (United States)

    Jiang, S Y; Li, L; Zhao, J; Xiang, Y; Wan, X R; Feng, F Z; Ren, T; Yang, J J

    2017-06-25

    Objective: To discuss the effects of prophylactic chemotherapy on the outcomes and prognosis of invasive mole patients. Methods: One hundred and fifteen invasive mole (IM) patients older than 40 years were registered in Peking Union Medical Collage Hospital.Eleven of them were treated with prophylactic chemotherapy before diagnosed as IM prophylactic chemotherapy group, while the other 104 cases received therapeutic chemotherapy after diagnosed as IM (non-prophylactic chemotherapy group). The general clinical data (including age, clinical stage, risk factor score), treatment, outcomes and relapse of patients were retrospectively compared between two groups. Results: (1) The age of prophylactic chemotherapy group and non-prophylactic chemotherapy group were (47±5) versus (46±4) years old. Ratio of clinical stageⅠ-Ⅱ were 3/11 versus 29.8% (31/104), clinical stage Ⅲ-Ⅳ were 8/11 versus 70.2% (73/104). Ratio of risk factor score 0-6 were 11/11 versus 84.6% (88/104), risk factor score >6 were 0 versus 15.4% (16/104). There were no significant statistical differences between two groups in age, clinical stage or risk factor score (all P> 0.05). (2) Treatment: the total chemotherapy courses between prophylactic chemotherapy group and non-prophylactic chemotherapy group (median 7 versus 5) were significantly different ( Z= 3.071, P= 0.002). There were no significant statistical differences between two groups in the chemotherapy courses until negative conversion of β-hCG, consolidation chemotherapy courses, total therapeutic chemotherapy courses or ratio of hysterectomy (all P> 0.05). (3) Outcomes and relapse: between the prophylactic chemotherapy group and the non-prophylactic chemotherapy group, the complete remission rate were 11/11 versus 98.1%(102/104), the relapse rate were 0 versus 1.0%(1/102). There were no significant difference between the two groups in outcomes or relapse rate ( P> 0.05). Conclusions: Prophylactic chemotherapy does not substantially

  9. Mola hidatiforme completa e eclâmpsia: relato de caso Complete hydatidiform mole and eclampsia: a case report

    Directory of Open Access Journals (Sweden)

    Izildinha Maestá

    2003-07-01

    Full Text Available O desenvolvimento de pré-eclâmpsia ou eclâmpsia antes da 20ª semana deve levar à suspeita de mola hidatiforme. Descrevemos um caso de mola hidatiforme completa (MHC e eclâmpsia concomitante em paciente com 20 anos que apresentava sangramento genital, anemia, tamanho uterino excessivo e cistos de ovário, associados a hipertensão arterial e proteinúria. Os níveis de b-hCG estavam elevados e a função tiroidiana, alterada. A ultra-sonografia mostrou-se compatível com MHC. Após o esvaziamento uterino apresentou cefaléia e alterações visuais, seguidas por convulsões tônico-clônicas que cessaram com sulfato de magnésio hepta-hidratado a 50%. No seguimento pós-molar foi diagnosticado tumor trofoblástico gestacional (TTG prontamente tratado com quimioterapia. A associação de MHC e eclâmpsia determina esvaziamento uterino imediato e seguimento pós-molar rigoroso, pelo risco aumentado de desenvolvimento de TTG.Development of preeclampsia/eclampsia prior to 20 weeks of pregnancy should raise the suspicion of hydatidiform mole. We report a case of complete hydatidiform mole (CHM concurrent with eclampsia in a 20-year-old patient with vaginal bleeding, anemia, large uterine size, and ovary cysts associated with hypertension and proteinuria. Plasmatic b-hCG levels were high and there was abnormal thyroid function. The ultrasonographic findings were compatible with CHM. After uterine evacuation, the patient had headache and visual alterations, followed by tonic-clonic seizures, which ceased with the administration of 50% magnesium sulfate. At post-molar follow-up, a gestational trophoblastic tumor (GTT was diagnosed and promptly treated with chemotherapy. Association between CHM and eclampsia requires immediate uterine evacuation and strict post-molar follow-up, due to increased risk of GTT development.

  10. Influence of 17β-estradiol treatment on the expression of NF-κB in complete hydatidiform mole culture

    Directory of Open Access Journals (Sweden)

    Tatit Nurseta

    2013-12-01

    Full Text Available Background:  Genetic evidence has established a role of nuclear factor kappa B (NF-κB signaling in oncogenesis. However, activity of NF-κB in complete hydatidiform mole (CHM cell culture under 17β-estradiol (E2 treatment is not yet known. Recently, a positive cross-talk between estrogen receptor (ER and NF-κB to promote survival and progress of cancer cells to a more aggressive phenotype was established. In the present study, we examined the influence of E2 treatment on the NF-κB expression in CHM’s culture.Methods:  This experimental study measured the expression of NF-κB in CHM culture treated with E2: 10, 100, 300, 600, and 1000 pg/mL and without E2. Imunohistochemistry staining was used to assess the expression of NF-κB. Microphotographs were taken using 400x magnification. Adobe photoshop CS2 was used to assess the NF-κB expression in cell nucleus. The lower the color intensity of cell RGBbv, is the higher the expression of NF-κB in cells. ANOVA test was performed to compare the expression of NF-κB.Results: NF-κB expression as indicated by color intensity in control group was 114.84 ± 9.02. NF-κB expression in E2 treatment groups were respectively: E2 10 pg/mL: 106.30 ± 13.95; E2 100 pg/mL: 82.47 ± 4.72; E2 300 pg/mL: 82.24 ± 2.67; E2 600 pg/mL: 69.05 ± 6.47; E2 1000 pg/mL: 68.49 ± 2.37. There was progressive decline in color intensity of cells with E2 treatment indicating the increase expression of NF-κB. Significant differences with the control group occurred in doses of E2 100, 300, 600, dan 1000 pg/mL.Conclusion: Treatment of CHM trophoblast culture with escalating doses of E2 was associated with the increase of NF-κB expression in a dose dependent manner. (Med J Indones. 2013;22:197-201. doi: 10.13181/mji.v22i4.599Keywords: 17-β Estradiol, Hydatidiform mole, NF-κB

  11. Quitosana no controle pós-colheita da podridão mole em caqui 'rama forte' Chitosan on the postharvest control of soft rot in 'rama forte' persimmon

    Directory of Open Access Journals (Sweden)

    Patrícia Cia

    2010-01-01

    Full Text Available Este trabalho objetivou avaliar o efeito da quitosana, aliada ao processo de destanização, no controle de Rhizopus stolonifer em caqui 'Rama Forte' e sobre o crescimento micelial do fungo in vitro. Caquis foram submetidos ao processo de destanização com CO2 (70% / 18 horas, em tambores herméticos, sendo em seguida submetidos à inoculação com suspensão de esporos de R. stolonifer (3x10(5 esporos mL-1. Após inoculação, os frutos permaneceram por 2 horas a 25 °C, quando foram imersos em quitosana (0; 0,5; 1,0; 1,5 e 2,0%, por 1 minuto. Os frutos foram mantidos a 25 °C / 80% UR e avaliados quanto à severidade e incidência da podridão mole, durante cinco dias. Após o período de armazenamento, avaliaram-se a coloração de casca, a firmeza e o índice de adstringência. In vitro, avaliou-se o crescimento micelial em placas contendo meio BDA incorporado com quitosana ou ácido cítrico. Os resultados mostraram que a quitosana, a 1,5%, reduz a severidade e a incidência da podridão mole em caquis e não influencia no processo de perda de adstringência, firmeza e na coloração de casca. In vitro, a quitosana inibe completamente o crescimento micelial de R. stolonifer, em concentração tão baixa quanto 0,5%.The objective of this research was to evaluate the effect of chitosan in addition to de-astringency process on the control of Rhizopus stolonifer in 'Rama Forte' persimmon and on in vitro mycelial growth. Persimmon were submitted to de-astringency process using CO2 (70% / 18 h, in hermetic chambers. Next, fruit were inoculated through subcuticular injections of a R. stolonifer spore suspension (3x10(5 spore mL-1 and 2 hours later at 25 °C immersed into chitosan (0, 0.5, 1.0, 1.5, and 2.0%, for 1 min. Fruit were stored at 25 °C / 80% RH, and checked for rot severity and incidence of soft rot, during 5-days of storage. After storage the skin color, firmness and astringency index were evaluated in persimmons. In vitro, mycelial

  12. A distinct role of the queen in coordinated workload and soil distribution in eusocial naked mole-rats.

    Directory of Open Access Journals (Sweden)

    Nobuyuki Kutsukake

    Full Text Available We investigated how group members achieve collective decision-making, by considering individual intrinsic behavioural rules and behavioural mechanisms for maintaining social integration. Using a simulated burrow environment, we investigated the behavioural rules of coordinated workload for soil distribution in a eusocial mammal, the naked mole-rat (Heterocephalus glaber. We tested two predictions regarding a distinct role of the queen, a socially dominant individual in the caste system: the presence of a queen would increase the workload of other caste individuals, and the cues by a queen would affect the soil distribution. In experiment 1, we placed four individuals of various castes from the same colony into an experimental burrow. Workers exhibited the highest frequency of workload compared to other castes. The presence of a queen activated the workload by other individuals. Individuals showed a consistent workload in a particular direction so as to bias the soil distribution. These results suggest that individuals have a consensus on soil distribution and that the queen plays a distinct role. In experiment 2, we placed the odour of a queen in one of four cells and observed its effect on other individuals' workload and soil distribution. Relative to other cells, individuals frequently dug in the queen cell so the amount of soil in the queen cell decreased. These results suggest that queen odour is an important cue in coordinated workload and soil distribution in this species.

  13. Recurrent hydatidiform mole: A case report of six consecutive molar pregnancies complicated by choriocarcinoma, and review of the literature

    Directory of Open Access Journals (Sweden)

    Ahlam A Al-Ghamdi

    2011-01-01

    Full Text Available Hydatidiform mole (HM is the most common form of gestational trophoblastic neoplasia. Recurrence of HM is extremely rare. Here, we report the case of a patient with six consecutive partial HMs without normal pregnancy. A 42-year-old lady who was referred to us at King Fahad Hospital of the University, Al Khobar, initially as a case of 26-year-old with persistent trophoblastic disease after three recurrent molar pregnancies that were confirmed histologically in the referring hospital. She underwent evacuation and curettage and was followed up by serial β-human chorionic gonadotropin levels, and did not require chemotherapy. She then had three more molar pregnancies in 1995, 1996, and 2004; all molar pregnancies were evacuated by suction curettage at her base hospital, but in the last event, she complained of shortness of breath and abdominal pain. Diagnostic workup in our hospital confirmed choriocarcinoma, for which she received multiple regimen chemotherapy and was cured. Unfortunately, she lately presented with symptoms suggestive of premature menopause.

  14. Assistência de enfermagem ao escolar: uma introdução ao problema

    Directory of Open Access Journals (Sweden)

    Maria das Graças C. Ferriani

    1983-12-01

    Full Text Available Neste estudo são apresentados alguns aspectos da assistência de saúde ao escolar através dos tempos. Atualmente, profissionais de saúde estão voltados para a saúde escolar e todos tentam de alguma forma desempenhar o seu papel contribuindo para transformar os escolares em cidadãos úteis e capazes. A enfermagem de há muito tempo despertou seu interesse para melhorar as condições de vida do escolar. Na literatura consultada verificamos que a enfermeira, em alguns países da Europa e Estados Unidos, faz parte integrante do Sistema Escolar, e esta atividade é oficializada pelo governo. No Brasil, embora exista uma lei que coloca a enfermeira no sistema escolar, não encontramos nenhuma atividade de enfermagem oficializada e próprias das Secretarias de Educação dos diversos setores da União. O objet'vo deste ensaio é mostrar a importância da assistência de enfermagem dentro do sistema escolar.

  15. Bilateral lesions of the medial frontal cortex disrupt recognition of social hierarchy during antiphonal communication in naked mole-rats (Heterocephalus glaber).

    Science.gov (United States)

    Yosida, Shigeto; Okanoya, Kazuo

    2012-02-01

    Generation of the motor patterns of emotional sounds in mammals occurs in the periaqueductal gray matter of the midbrain and is not directly controlled by the cortex. The medial frontal cortex indirectly controls vocalizations, based on the recognition of social context. We examined whether the medial frontal cortex was responsible for antiphonal vocalization, or turn-taking, in naked mole-rats. In normal turn-taking, naked mole-rats vocalize more frequently to dominant individuals than to subordinate ones. Bilateral lesions of the medial frontal cortex disrupted differentiation of call rates to the stimulus animals, which had varied social relationships to the subject. However, medial frontal cortex lesions did not affect either the acoustic properties of the vocalizations or the timing of the vocal exchanges. This suggests that the medial frontal cortex may be involved in social cognition or decision making during turn-taking, while other regions of the brain regulate when animals vocalize and the vocalizations themselves.

  16. Numerical Investigation of the Effect of C/O Mole Ratio on the Performance of Rotary Hearth Furnace Using a Combined Model

    Science.gov (United States)

    Liu, Ying; Wen, Zhi; Lou, Guofeng; Li, Zhi; Yong, Haiquan; Feng, Xiaohong

    2014-12-01

    In a rotary hearth furnace (RHF) the direct reduction of composite pellets and processes of heat and mass transfer as well as combustion in the chamber of RHF influence each other. These mutual interactions should be considered when an accurate model of RHF is established. This paper provides a combined model that incorporates two sub-models to investigate the effects of C/O mole ratio in the feed pellets on the reduction kinetics and heat and mass transfer as well as combustion processes in the chamber of a pilot-scale RHF. One of the sub-models is established to describe the direct reduction process of composite pellets on the hearth of RHF. Heat and mass transfer within the pellet, chemical reactions, and radiative heat transfer from furnace walls and combustion gas to the surface of the pellet are considered in the model. The other sub-model is used to simulate gas flow and combustion process in the chamber of RHF by using commercial CFD software, FLUENT. The two sub-models were linked through boundary conditions and heat, mass sources. Cases for pellets with different C/O mole ratio were calculated by the combined model. The calculation results showed that the degree of metallization, the total amounts of carbon monoxide escaping from the pellet, and heat absorbed by chemical reactions within the pellet as well as CO and CO2 concentrations in the furnace increase with the increase of C/O mole ratio ranging from 0.6 to 1.0, when calculation conditions are the same except for C/O molar ratio. Carbon content in the pellet has little influence on temperature distribution in the furnace under the same calculation conditions except for C/O mole ratio in the feed pellets.

  17. The long gestation of the small naked mole-rat (Heterocephalus glaber Rüppell, 1842) studied with ultrasound biomicroscopy and 3D-ultrasonography.

    Science.gov (United States)

    Roellig, Kathleen; Drews, Barbara; Goeritz, Frank; Hildebrandt, Thomas Bernd

    2011-03-07

    The naked mole-rat (Heterocephalus glaber) is one of the two known mammalian species that live in a eusocial population structure. Here we investigate the exceptionally long gestation period of 70 days observed in the mole-rat queen. The course of seven successful pregnancies in two individuals was recorded in a colony of captive naked mole-rats using ultrasound biomicroscopy (UBM) and 3D-ultrasonography. We establish a catalogue of basic reference ultrasound data for this species by describing the ultrasonographic appearance of reproductive organs, calculating growth curves to predict gestational age and defining ultrasonographic milestones to characterize pregnancy stages. Mean litter size was 10.9±2.7, of which 7.2±1.5 survived the weaning period. Mean interbirth interval was 128.8±63.0 days. The reproductive success in our colony did not differ from previously published data. In the queen the active corpora lutea had an anechoic, fluid filled centre. Using UBM, pregnancy could be detected 53 days before parturition. The period of embryonic development is assumed to last until 30 days before parturition. Embryonic resorptions were detected frequently in the queen, indicating that this might be an ordinary event in this species. We discuss the extraordinary long gestation period of this small rodent and postulate that the long gestation is beneficial to both the eusocial structure and longevity. An increased litter size, twice as large as for other rodents of similar size, seemingly compensates for the doubling of pregnancy length. We demonstrate that the lifetime reproductive effort of a naked mole-rat queen is equivalent to the mass of offspring that would be produced if all of the females of a colony would be reproducing.

  18. The long gestation of the small naked mole-rat (Heterocephalus glaber Ruppell, 1842 studied with ultrasound biomicroscopy and 3D-ultrasonography.

    Directory of Open Access Journals (Sweden)

    Kathleen Roellig

    Full Text Available The naked mole-rat (Heterocephalus glaber is one of the two known mammalian species that live in a eusocial population structure. Here we investigate the exceptionally long gestation period of 70 days observed in the mole-rat queen. The course of seven successful pregnancies in two individuals was recorded in a colony of captive naked mole-rats using ultrasound biomicroscopy (UBM and 3D-ultrasonography. We establish a catalogue of basic reference ultrasound data for this species by describing the ultrasonographic appearance of reproductive organs, calculating growth curves to predict gestational age and defining ultrasonographic milestones to characterize pregnancy stages. Mean litter size was 10.9±2.7, of which 7.2±1.5 survived the weaning period. Mean interbirth interval was 128.8±63.0 days. The reproductive success in our colony did not differ from previously published data. In the queen the active corpora lutea had an anechoic, fluid filled centre. Using UBM, pregnancy could be detected 53 days before parturition. The period of embryonic development is assumed to last until 30 days before parturition. Embryonic resorptions were detected frequently in the queen, indicating that this might be an ordinary event in this species. We discuss the extraordinary long gestation period of this small rodent and postulate that the long gestation is beneficial to both the eusocial structure and longevity. An increased litter size, twice as large as for other rodents of similar size, seemingly compensates for the doubling of pregnancy length. We demonstrate that the lifetime reproductive effort of a naked mole-rat queen is equivalent to the mass of offspring that would be produced if all of the females of a colony would be reproducing.

  19. Tunable diode laser absorption spectroscopy-based tomography system for on-line monitoring of two-dimensional distributions of temperature and H2O mole fraction

    International Nuclear Information System (INIS)

    Xu, Lijun; Liu, Chang; Jing, Wenyang; Cao, Zhang; Xue, Xin; Lin, Yuzhen

    2016-01-01

    To monitor two-dimensional (2D) distributions of temperature and H 2 O mole fraction, an on-line tomography system based on tunable diode laser absorption spectroscopy (TDLAS) was developed. To the best of the authors’ knowledge, this is the first report on a multi-view TDLAS-based system for simultaneous tomographic visualization of temperature and H 2 O mole fraction in real time. The system consists of two distributed feedback (DFB) laser diodes, a tomographic sensor, electronic circuits, and a computer. The central frequencies of the two DFB laser diodes are at 7444.36 cm −1 (1343.3 nm) and 7185.6 cm −1 (1391.67 nm), respectively. The tomographic sensor is used to generate fan-beam illumination from five views and to produce 60 ray measurements. The electronic circuits not only provide stable temperature and precise current controlling signals for the laser diodes but also can accurately sample the transmitted laser intensities and extract integrated absorbances in real time. Finally, the integrated absorbances are transferred to the computer, in which the 2D distributions of temperature and H 2 O mole fraction are reconstructed by using a modified Landweber algorithm. In the experiments, the TDLAS-based tomography system was validated by using asymmetric premixed flames with fixed and time-varying equivalent ratios, respectively. The results demonstrate that the system is able to reconstruct the profiles of the 2D distributions of temperature and H 2 O mole fraction of the flame and effectively capture the dynamics of the combustion process, which exhibits good potential for flame monitoring and on-line combustion diagnosis

  20. Long-term analysis of carbon dioxide and methane column-averaged mole fractions retrieved from SCIAMACHY

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    O. Schneising

    2011-03-01

    Full Text Available Carbon dioxide (CO2 and methane (CH4 are the two most important anthropogenic greenhouse gases contributing to global climate change. SCIAMACHY onboard ENVISAT (launch 2002 was the first and is now with TANSO onboard GOSAT (launch 2009 one of only two satellite instruments currently in space whose measurements are sensitive to CO2 and CH4 concentration changes in the lowest atmospheric layers where the variability due to sources and sinks is largest.

    We present long-term SCIAMACHY retrievals (2003–2009 of column-averaged dry air mole fractions of both gases (denoted XCO2 and XCH4 derived from absorption bands in the near-infrared/shortwave-infrared (NIR/SWIR spectral region focusing on large-scale features. The results are obtained using an upgraded version (v2 of the retrieval algorithm WFM-DOAS including several improvements, while simultaneously maintaining its high processing speed. The retrieved mole fractions are compared to global model simulations (CarbonTracker XCO2 and TM5 XCH4 being optimised by assimilating highly accurate surface measurements from the NOAA/ESRL network and taking the SCIAMACHY averaging kernels into account. The comparisons address seasonal variations and long-term characteristics.

    The steady increase of atmospheric carbon dioxide primarily caused by the burning of fossil fuels can be clearly observed with SCIAMACHY globally. The retrieved global annual mean XCO2 increase agrees with CarbonTracker within the error bars (1.80±0.13 ppm yr−1 compared to 1.81±0.09 ppm yr−1. The amplitude of the XCO2 seasonal cycle as retrieved by SCIAMACHY, which is 4.3±0.2 ppm for the Northern Hemisphere and 1.4±0.2 ppm for the Southern Hemisphere, is on average about 1 ppm larger than for CarbonTracker.

    An investigation of the boreal forest carbon uptake during the

  1. Reduced Utilization of Selenium by Naked Mole Rats Due to a Specific Defect in GPx1 Expression*

    Science.gov (United States)

    Kasaikina, Marina V.; Lobanov, Alexei V.; Malinouski, Mikalai Y.; Lee, Byung Cheon; Seravalli, Javier; Fomenko, Dmitri E.; Turanov, Anton A.; Finney, Lydia; Vogt, Stefan; Park, Thomas J.; Miller, Richard A.; Hatfield, Dolph L.; Gladyshev, Vadim N.

    2011-01-01

    Naked mole rat (MR) Heterocephalus glaber is a rodent model of delayed aging because of its unusually long life span (>28 years). It is also not known to develop cancer. In the current work, tissue imaging by x-ray fluorescence microscopy and direct analyses of trace elements revealed low levels of selenium in the MR liver and kidney, whereas MR and mouse brains had similar selenium levels. This effect was not explained by uniform selenium deficiency because methionine sulfoxide reductase activities were similar in mice and MR. However, glutathione peroxidase activity was an order of magnitude lower in MR liver and kidney than in mouse tissues. In addition, metabolic labeling of MR cells with 75Se revealed a loss of the abundant glutathione peroxidase 1 (GPx1) band, whereas other selenoproteins were preserved. To characterize the MR selenoproteome, we sequenced its liver transcriptome. Gene reconstruction revealed standard selenoprotein sequences except for GPx1, which had an early stop codon, and SelP, which had low selenocysteine content. When expressed in HEK 293 cells, MR GPx1 was present in low levels, and its expression could be rescued neither by removing the early stop codon nor by replacing its SECIS element. In addition, GPx1 mRNA was present in lower levels in MR liver than in mouse liver. To determine if GPx1 deficiency could account for the reduced selenium content, we analyzed GPx1 knock-out mice and found reduced selenium levels in their livers and kidneys. Thus, MR is characterized by the reduced utilization of selenium due to a specific defect in GPx1 expression. PMID:21372135

  2. Awareness of sunburn in childhood, use of sunbeds and change of moles in Denmark, Northern Ireland, Norway and Sweden.

    Science.gov (United States)

    Hajdarevic, Senada; Hvidberg, Line; Lin, Yulan; Donnelly, Conan; Gavin, Anna; Lagerlund, Magdalena; Pedersen, Anette F; Rasmussen, Birgit H; Runesdotter, Sara; Vedsted, Peter; Tishelman, Carol

    2016-02-01

    Malignant melanoma (MM) is increasing rapidly in Northern Europe. To reduce incidence and mortality through earlier diagnosis, public awareness of MM is important. Thus, we aim to examine awareness of risk factors and a symptom of MM, and how awareness varies by country and socio-demographic factors in Denmark, Northern Ireland (NI), Norway and Sweden. Population-based telephone interviews using the 'Awareness and Beliefs about Cancer' measure were conducted in 2011 among 8355 adults ≥50 years as part of the International Cancer Benchmarking Partnership Module 2. Prevalence ratios (PRs) with 95% confidence intervals were calculated. In these four countries, lowest awareness was found for 'sunburn in childhood' (63%), whereas awareness was high for 'use of sunbeds' (91%) and 'mole change' (97%). Lack of awareness of 'sunburn in childhood' was more prevalent among respondents from Norway [PR = 1.38 (1.28-1.48)] but less prevalent among respondents from Northern Ireland (NI) [PR = 0.78 (0.72-0.85)] and Sweden [PR = 0.86 (0.79-0.93)] compared with respondents from Denmark. Lack of awareness of 'use of sunbeds' was more prevalent among respondents from Norway [PR = 2.99 (2.39-3.74)], Sweden [PR = 1.57 (1.22-2.00)], and NI [PR = 1.65 (1.30-2.10)] compared with respondents form Denmark. Being a man, age ≥70, living alone, and having lower education, were each independently associated with lack of MM-awareness. The results indicate relatively low awareness of 'sunburn in childhood' as a risk factor for MM, and important disparities in MM-awareness across countries and socio-demographic groups. Improved and more directed initiatives to enhance public MM-awareness, particularly about 'sunburn in childhood', are needed. © The Author 2015. Published by Oxford University Press on behalf of the European Public Health Association. All rights reserved.

  3. Immunohistochemical characterization of the chemosensory pulmonary neuroepithelial bodies in the naked mole-rat reveals a unique adaptive phenotype.

    Directory of Open Access Journals (Sweden)

    Jie Pan

    Full Text Available The pulmonary neuroepithelial bodies (NEBs constitute polymodal airway chemosensors for monitoring and signaling ambient gas concentrations (pO2, pCO2/H+ via complex innervation to the brain stem controlling breathing. NEBs produce the bioactive amine, serotonin (5-HT, and a variety of peptides with multiple effects on lung physiology and other organ systems. NEBs in mammals appear prominent and numerous during fetal and neonatal periods, and decline in the post-natal period suggesting an important role during perinatal adaptation. The naked mole-rat (NMR, Heterocephalus glaber, has adapted to the extreme environmental conditions of living in subterranean burrows in large colonies (up to 300 colony mates. The crowded, unventilated burrows are environments of severe hypoxia and hypercapnia. However, NMRs adjust readily to above ground conditions. The chemosensory NEBs of this species were characterized and compared to those of the conventional Wistar rat (WR to identify similarities and differences that could explain the NMR's adaptability to environments. A multilabel immunohistochemical analysis combined with confocal microscopy revealed that the expression patterns of amine, peptide, neuroendocrine, innervation markers and chemosensor component proteins in NEBs of NMR were similar to that of WR. However, we found the following differences: 1 NEBs in both neonatal and adult NMR lungs were significantly larger and more numerous as compared to WR; 2 NEBs in NMR had a more variable compact cell organization and exhibited significant differences in the expression of adhesion proteins; 3 NMR NEBs showed a significantly greater ratio of 5-HT positive cells with an abundance of 5-HT; 4 NEBs in NMR expressed the proliferating cell nuclear antigen (PCNA and the neurogenic gene (MASH1 indicating active proliferation and a state of persistent differentiation. Taken together our findings suggest that NEBs in lungs of NMR are in a hyperactive, functional

  4. They live in the land down under: thyroid function and basal metabolic rate in the Blind Mole Rat, Spalax.

    Science.gov (United States)

    Avivi, Aaron; Nevo, Eviatar; Cohen, Keren; Sotnichenko, Nick; Hercbergs, Aleck; Band, Mark; Davis, Paul J; Ellis, Martin; Ashur-Fabian, Osnat

    2014-01-01

    The Israeli blind subterranean mole rat (Spalax ehrenbergi superspecies) lives in sealed underground burrows under extreme, hypoxic conditions. The four Israeli Spalax allospecies have adapted to different climates, the cool-humid (Spalax galili, 2 n = 52 chromosomes), semihumid (S. golani, 2 n = 54) north regions, warm-humid (S. carmeli, 2 n = 58) central region and the warm-dry S. judaei, 2 n = 60) southern regions. A dramatic interspecies decline in basal metabolic rate (BMR) from north to south, even after years of captivity, indicates a genetic basis for this BMR trait. We examined the possibility that the genetically-conditioned interspecies BMR difference was expressed via circulating thyroid hormone. An unexpected north to south increase in serum free thyroxine (FT4) and total 3, 5, 3'-triiodo-L-thyronine (T3) (p BMR measurements. The increases in serum FT4 and T3 were symmetrical, so that the T3:FT4 ratio - interpretable as an index of conversion of T4 to T3 in nonthyroidal tissues - did not support relative decrease in production of T3 as a contributor to BMR. Increased north-to-south serum FT4 and T3 levels also correlated negatively with hemoglobin/hematocrit. North-to-south adaptations in spalacids include decreased BMR and hematocrit/hemoglobin in the face of increasing thyroid hormone levels, arguing for independent control of hormone secretion and BMR/hematocrit/hemoglobin. But the significant inverse relationship between thyroid hormone levels and BMR/hematocrit/hemoglobin is also consistent with a degree of cellular resistance to thyroid hormone action that protects against hormone-induced increase in oxygen consumption in a hostile, hypoxic environment.

  5. Removal of reproductive suppression reveals latent sex differences in brain steroid hormone receptors in naked mole-rats, Heterocephalus glaber.

    Science.gov (United States)

    Swift-Gallant, Ashlyn; Mo, Kaiguo; Peragine, Deane E; Monks, D Ashley; Holmes, Melissa M

    2015-01-01

    Naked mole-rats are eusocial mammals, living in large colonies with a single breeding female and 1-3 breeding males. Breeders are socially dominant, and only the breeders exhibit traditional sex differences in circulating gonadal steroid hormones and reproductive behaviors. Non-reproductive subordinates also fail to show sex differences in overall body size, external genital morphology, and non-reproductive behaviors. However, subordinates can transition to breeding status if removed from their colony and housed with an opposite-sex conspecific, suggesting the presence of latent sex differences. Here, we assessed the expression of steroid hormone receptor and aromatase messenger RNA (mRNA) in the brains of males and females as they transitioned in social and reproductive status. We compared in-colony subordinates to opposite-sex subordinate pairs that were removed from their colony for either 1 day, 1 week, 1 month, or until they became breeders (i.e., produced a litter). Diencephalic tissue was collected and mRNA of androgen receptor (Ar), estrogen receptor alpha (Esr1), progesterone receptor (Pgr), and aromatase (Cyp19a1) was measured using qPCR. Testosterone, 17β-estradiol, and progesterone from serum were also measured. As early as 1 week post-removal, males exhibited increased diencephalic Ar mRNA and circulating testosterone, whereas females had increased Cyp19a1 mRNA in the diencephalon. At 1 month post-removal, females exhibited increased 17β-estradiol and progesterone. The largest changes in steroid hormone receptors were observed in breeders. Breeding females had a threefold increase in Cyp19a1 and fivefold increases in Esr1 and Pgr, whereas breeding males had reduced Pgr and increased Ar. These data demonstrate that sex differences in circulating gonadal steroids and hypothalamic gene expression emerge weeks to months after subordinate animals are removed from reproductive suppression in their home colony.

  6. Immunohistochemical characterization of the chemosensory pulmonary neuroepithelial bodies in the naked mole-rat reveals a unique adaptive phenotype.

    Science.gov (United States)

    Pan, Jie; Park, Thomas J; Cutz, Ernest; Yeger, Herman

    2014-01-01

    The pulmonary neuroepithelial bodies (NEBs) constitute polymodal airway chemosensors for monitoring and signaling ambient gas concentrations (pO2, pCO2/H+) via complex innervation to the brain stem controlling breathing. NEBs produce the bioactive amine, serotonin (5-HT), and a variety of peptides with multiple effects on lung physiology and other organ systems. NEBs in mammals appear prominent and numerous during fetal and neonatal periods, and decline in the post-natal period suggesting an important role during perinatal adaptation. The naked mole-rat (NMR), Heterocephalus glaber, has adapted to the extreme environmental conditions of living in subterranean burrows in large colonies (up to 300 colony mates). The crowded, unventilated burrows are environments of severe hypoxia and hypercapnia. However, NMRs adjust readily to above ground conditions. The chemosensory NEBs of this species were characterized and compared to those of the conventional Wistar rat (WR) to identify similarities and differences that could explain the NMR's adaptability to environments. A multilabel immunohistochemical analysis combined with confocal microscopy revealed that the expression patterns of amine, peptide, neuroendocrine, innervation markers and chemosensor component proteins in NEBs of NMR were similar to that of WR. However, we found the following differences: 1) NEBs in both neonatal and adult NMR lungs were significantly larger and more numerous as compared to WR; 2) NEBs in NMR had a more variable compact cell organization and exhibited significant differences in the expression of adhesion proteins; 3) NMR NEBs showed a significantly greater ratio of 5-HT positive cells with an abundance of 5-HT; 4) NEBs in NMR expressed the proliferating cell nuclear antigen (PCNA) and the neurogenic gene (MASH1) indicating active proliferation and a state of persistent differentiation. Taken together our findings suggest that NEBs in lungs of NMR are in a hyperactive, functional and

  7. Pigmentary Markers in Danes--Associations with Quantitative Skin Colour, Nevi Count, Familial Atypical Multiple-Mole, and Melanoma Syndrome.

    Directory of Open Access Journals (Sweden)

    Peter Johansen

    Full Text Available To investigate whether pigmentation genes involved in the melanogenic pathway (melanogenesis contributed to melanoma predisposition, we compared pigmentary genetics with quantitative skin pigmentation measurements, the number of atypical nevi, the total nevus count, and the familial atypical multiple mole and melanoma (FAMMM syndrome. We typed 32 pigmentary SNP markers and sequenced MC1R in 246 healthy individuals and 116 individuals attending periodic control for malignant melanoma development, 50 of which were diagnosed with FAMMM. It was observed that individuals with any two grouped MC1R variants (missense, NM_002386:c. 456C > A (p.TYR152*, or NM_002386:c.83_84insA (p.Asn29Glnfs*14 had significantly (p<0.001 lighter skin pigmentation of the upper-inner arm than those with none or one MC1R variant. We did not observe any significant association of the MC1R variants with constitutive pigmentation measured on the buttock area. We hypothesize that the effect of MC1R variants on arm pigmentation is primarily reflecting the inability to tan when subjected to UVR. A gender specific effect on skin pigmentation was also observed, and it was found that the skin pigmentation of females on average were darker than that of males (p<0.01. We conclude that MC1R variants are associated with quantitative skin colour in a lightly pigmented Danish population. We did not observe any association between any pigmentary marker and the FAMMM syndrome. We suggest that the genetics of FAMMM is not related to the genetics of the pigmentary pathway.

  8. Theoretical paper: exploring overlooked natural mitochondria-rejuvenative intervention: the puzzle of bowhead whales and naked mole rats.

    Science.gov (United States)

    Prokopov, Arkadi F

    2007-12-01

    There is an imperative need for exploring and implementing mitochondria-rejuvenative interventions that can bridge the current gap toward the step-by step realization of strategies for engineered negligible senescence (SENS) agenda. Recently discovered in mammals, natural mechanism mitoptosis-a selective "suicide" of mutated mitochondria-can facilitate continuous purification of mitochondrial pool in an organism from the most reactive oxygen species (ROS)-producing mitochondria. Mitoptosis, which is considered to be the first stage of ROS-induced apoptosis, underlies follicular atresia (a "quality control" mechanism in female germline cells that eliminates most germinal follicles in female embryos). Mitoptosis can be also activated in adult postmitotic somatic cells by evolutionary conserved phenotypic adaptations to intermittent oxygen restriction (IOR) and synergistically acting intermittent caloric restriction (ICR). IOR and ICR are common in mammals and seem to underlie extraordinary longevity and augmented cancer resistance in bowhead whales (Balena mysticetus) and naked mole rats (Heterocephalus glaber). Furthermore, in mammals IOR can facilitate continuous stromal stem cells-de-pendent tissue repair. A comparative analysis of IOR and ICR mechanisms in both mammals, in conjunction with the experience of decades of biomedical and clinical research on emerging preventative, therapeutic, and rehabilitative modality-the intermittent hypoxic training/therapy (IHT)-indicates that the notable clinical efficiency of IHT is based on the universal adaptational mechanisms that are common in mammals. Further exploration of natural mitochondria-preserving and -rejuvenating strategies can help refinement of IOR- and ICR-based synergistic protocols, having value in clinical human rejuvenation.

  9. As sementes geneticamente modificadas e o direito ao desenvolvimento

    Directory of Open Access Journals (Sweden)

    Milena Barbosa Melo

    2017-12-01

    Full Text Available O presente artigo apresenta como objeto de investigação a ligação entre os direitos da propriedade intelectual no contexto dos organismos geneticamente modificados (OGM´S. Objetivou-se, especificamente, responder inquietações acerca dos danos reais para os consumidores, bem como o lucro para as empresas multinacionais, sem desconsiderar, ainda, os efetivos danos ao meio ambiente e à saúde. Utilizou metodologia pautada em pesquisa bibliográfica e documental a partir do método hermenêutico fazendo uso da interpretação judicial-histórica. Conclui pela relevância da temática para efetivação do direito ao desenvolvimento, bem como pela urgência de uma regulamentação da biossegurança, de forma a permitir melhor sustentação aos OGM´s.

  10. Adesão ao tratamento no transtorno bipolar

    OpenAIRE

    Santin,Aida; Ceresér,Keila; Rosa,Adriane

    2005-01-01

    O transtorno bipolar (TB) é uma doença crônica, recorrente, presente em 1,5% da população, estando associada a altas taxas de mortalidade e prejuízos socioeconômicos. O lítio, a carbamazepina e o ácido valpróico são os estabilizadores de humor mais usados. Em tratamentos prolongados, como é o caso do TB, a má-adesão dos pacientes é uma das maiores dificuldades. Verifica-se que fatores ligados ao paciente, aos medicamentos e aos médicos possam ser responsáveis pela baixa adesão. A psicoeducaçã...

  11. An energy stable evolution method for simulating two-phase equilibria of multi-component fluids at constant moles, volume and temperature

    KAUST Repository

    Kou, Jisheng

    2016-02-25

    In this paper, we propose an energy-stable evolution method for the calculation of the phase equilibria under given volume, temperature, and moles (VT-flash). An evolution model for describing the dynamics of two-phase fluid system is based on Fick’s law of diffusion for multi-component fluids and the Peng-Robinson equation of state. The mobility is obtained from diffusion coefficients by relating the gradient of chemical potential to the gradient of molar density. The evolution equation for moles of each component is derived using the discretization of diffusion equations, while the volume evolution equation is constructed based on the mechanical mechanism and the Peng-Robinson equation of state. It is proven that the proposed evolution system can well model the VT-flash problem, and moreover, it possesses the property of total energy decay. By using the Euler time scheme to discretize this evolution system, we develop an energy stable algorithm with an adaptive choice strategy of time steps, which allows us to calculate the suitable time step size to guarantee the physical properties of moles and volumes, including positivity, maximum limits, and correct definition of the Helmhotz free energy function. The proposed evolution method is also proven to be energy-stable under the proposed time step choice. Numerical examples are tested to demonstrate efficiency and robustness of the proposed method.

  12. Effect of the growth temperature and the AlN mole fraction on In incorporation and properties of quaternary III-nitride layers grown by molecular beam epitaxy

    International Nuclear Information System (INIS)

    Fernandez-Garrido, S.; Pereiro, J.; Munoz, E.; Calleja, E.; Redondo-Cubero, A.; Gago, R.; Bertram, F.; Christen, J.; Luna, E.; Trampert, A.

    2008-01-01

    Indium incorporation into wurtzite (0001)-oriented In x Al y Ga 1-x-y N layers grown by plasma-assisted molecular beam epitaxy was studied as a function of the growth temperature (565-635 deg. C) and the AlN mole fraction (0.01< y<0.27). The layer stoichiometry was determined by Rutherford backscattering spectrometry (RBS). RBS shows that indium incorporation decreased continuously with increasing growth temperature due to thermally enhanced dissociation of In-N bonds and for increasing AlN mole fractions. High resolution x-ray diffraction and transmission electron microscopy (TEM) measurements did not show evidence of phase separation. The mosaicity of the quaternary layers was found to be mainly determined by the growth temperature and independent on alloy composition within the range studied. However, depending on the AlN mole fraction, nanometer-sized composition fluctuations were detected by TEM. Photoluminescence spectra showed a single broad emission at room temperature, with energy and bandwidth S- and W-shaped temperature dependences typical of exciton localization by alloy inhomogeneities. Cathodoluminescence measurements demonstrated that the alloy inhomogeneities, responsible of exciton localization, occur on a lateral length scale below 150 nm, which is corroborated by TEM

  13. Low sulfide levels and a high degree of cystathionine β-synthase (CBS) activation by S-adenosylmethionine (SAM) in the long-lived naked mole-rat.

    Science.gov (United States)

    Dziegelewska, Maja; Holtze, Susanne; Vole, Christiane; Wachter, Ulrich; Menzel, Uwe; Morhart, Michaela; Groth, Marco; Szafranski, Karol; Sahm, Arne; Sponholz, Christoph; Dammann, Philip; Huse, Klaus; Hildebrandt, Thomas; Platzer, Matthias

    2016-08-01

    Hydrogen sulfide (H2S) is a gaseous signalling molecule involved in many physiological and pathological processes. There is increasing evidence that H2S is implicated in aging and lifespan control in the diet-induced longevity models. However, blood sulfide concentration of naturally long-lived species is not known. Here we measured blood sulfide in the long-lived naked mole-rat and five other mammalian species considerably differing in lifespan and found a negative correlation between blood sulfide and maximum longevity residual. In addition, we show that the naked mole-rat cystathionine β-synthase (CBS), an enzyme whose activity in the liver significantly contributes to systemic sulfide levels, has lower activity in the liver and is activated to a higher degree by S-adenosylmethionine compared to other species. These results add complexity to the understanding of the role of H2S in aging and call for detailed research on naked mole-rat transsulfuration. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  14. Low sulfide levels and a high degree of cystathionine β-synthase (CBS activation by S-adenosylmethionine (SAM in the long-lived naked mole-rat

    Directory of Open Access Journals (Sweden)

    Maja Dziegelewska

    2016-08-01

    Full Text Available Hydrogen sulfide (H2S is a gaseous signalling molecule involved in many physiological and pathological processes. There is increasing evidence that H2S is implicated in aging and lifespan control in the diet-induced longevity models. However, blood sulfide concentration of naturally long-lived species is not known. Here we measured blood sulfide in the long-lived naked mole-rat and five other mammalian species considerably differing in lifespan and found a negative correlation between blood sulfide and maximum longevity residual. In addition, we show that the naked mole-rat cystathionine β-synthase (CBS, an enzyme whose activity in the liver significantly contributes to systemic sulfide levels, has lower activity in the liver and is activated to a higher degree by S-adenosylmethionine compared to other species. These results add complexity to the understanding of the role of H2S in aging and call for detailed research on naked mole-rat transsulfuration.

  15. The effects of oxotremorine, epibatidine, atropine, mecamylamine and naloxone in the tail-flick, hot-plate, and formalin tests in the naked mole-rat (Heterocephalus glaber).

    Science.gov (United States)

    Dulu, Thomas D; Kanui, Titus I; Towett, Philemon K; Maloiy, Geoffrey M; Abelson, Klas S P

    2014-01-01

    The naked mole-rat (Heterocephalus glaber) is a promising animal model for the study of pain mechanisms, therefore a thorough characterization of this species is essential. The aim of the present study was to establish the naked mole-rat as a model for studying the cholinergic receptor system in antinociception by investigating the involvement of muscarinic, nicotinic and opioid receptors in nociceptive tests in this species. The effects of systemic administration of the muscarinic receptor agonist oxotremorine and the nicotinic receptor agonist epibatidine were investigated in the tail-flick, the hot-plate, and the formalin tests. The effects of co-administration of the muscarinic receptor antagonist atropine, the nicotinic receptor antagonist mecamylamine, and the opioid receptor antagonist naloxone were also investigated. Oxotremorine and epibatidine induced a significant, dose-dependent antinociceptive effect in the tail-flick, hot-plate, and formalin tests, respectively. The effects of oxotremorine and epibatidine were blocked by atropine and mecamylamine, respectively. In all three nociceptive tests, naloxone in combination with oxotremorine or epibatidine enhanced the antinociceptive effects of the drugs. The present study demonstrated that stimulation of muscarinic and nicotinic receptors produces antinociceptive effects in the naked-mole rat. The reversal effect of atropine and mecamylamine suggests that this effect is mediated by cholinergic receptors. As naloxone increases the antinociceptive effects of cholinergic agonists, it is suggested that the cholinergic antinociception acts via a gateway facilitated by opioid receptor blockage; however, the precise interaction between these receptor systems needs further investigation.

  16. A window into extreme longevity; the circulating metabolomic signature of the naked mole-rat, a mammal that shows negligible senescence.

    Science.gov (United States)

    Lewis, Kaitlyn N; Rubinstein, Nimrod D; Buffenstein, Rochelle

    2018-04-20

    Mouse-sized naked mole-rats (Heterocephalus glaber), unlike other mammals, do not conform to Gompertzian laws of age-related mortality; adults show no age-related change in mortality risk. Moreover, we observe negligible hallmarks of aging with well-maintained physiological and molecular functions, commonly altered with age in other species. We questioned whether naked mole-rats, living an order of magnitude longer than laboratory mice, exhibit different plasma metabolite profiles, which could then highlight novel mechanisms or targets involved in disease and longevity. Using a comprehensive, unbiased metabolomics screen, we observe striking inter-species differences in amino acid, peptide, and lipid metabolites. Low circulating levels of specific amino acids, particularly those linked to the methionine pathway, resemble those observed during the fasting period at late torpor in hibernating ground squirrels and those seen in longer-lived methionine-restricted rats. These data also concur with metabolome reports on long-lived mutant mice, including the Ames dwarf mice and calorically restricted mice, as well as fruit flies, and even show similarities to circulating metabolite differences observed in young human adults when compared to older humans. During evolution, some of these beneficial nutrient/stress response pathways may have been positively selected in the naked mole-rat. These observations suggest that interventions that modify the aging metabolomic profile to a more youthful one may enable people to lead healthier and longer lives.

  17. Is Ki-67 of Diagnostic Value in Distinguishing Between Partial and Complete Hydatidiform Moles? A Systematic Review and Meta-analysis.

    Science.gov (United States)

    Zhao, Yue; Xiong, Guang-Wu; Zhang, Xiao-Wei; Hang, B O

    2018-02-01

    To demonstrate the value of Ki-67 in distinguishing between partial and complete hydatidiform moles. We searched electronic databases included Medline, WOK, Cochrane Library and CNKI, through January 24, 2015. Experts were consulted, and references from related articles were examined. The meta-analysis was conducted with RevMan5.3, according to the PRISMA guidelines. Mantel-Haenszel estimates were calculated and pooled under a random effect model, with data expressed as odds ratio (OR) and 95% confidence interval (CI). We analyzed eight trials with a total of 337 participants who underwent uterine curettage and met the inclusion criteria. A significantly higher expression of Ki-67 was observed in complete than in partial hydatidiform moles (OR=3.28; 95%CI=1.80-5.96; pvalue in distinguishing between partial and complete hydatidiform moles. However, the present study had only a limited number of samples, so investigation of a greater number of cases is needed to confirm this conclusion. Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

  18. Human chorionic gonadotrophin regression rate as a predictive factor of postmolar gestational trophoblastic neoplasm in high-risk hydatidiform mole: a case-control study.

    Science.gov (United States)

    Kim, Bo Wook; Cho, Hanbyoul; Kim, Hyunki; Nam, Eun Ji; Kim, Sang Wun; Kim, Sunghoon; Kim, Young Tae; Kim, Jae-Hoon

    2012-01-01

    The aim of this study was early prediction of postmolar gestational trophoblastic neoplasm (GTN) after evacuation of high-risk mole, by comparison of human chorionic gonadotrophin (hCG) regression rates. Fifty patients with a high-risk mole initially and spontaneously regressing after molar evacuation were selected from January 1, 1996 to May 31, 2010 (spontaneous regression group). Fifty patients with a high-risk mole initially and progressing to postmolar GTN after molar evacuation were selected (postmolar GTN group). hCG regression rates represented as hCG/initial hCG were compared between the two groups. The sensitivity and specificity of these rates for prediction of postmolar GTN were assessed using receiver operating characteristic curves. Multivariate analyses of associations between risk factors and postmolar GTN progression were performed. The mean regression rate of hCG between the two groups was compared. hCG regression rates represented as hCG/initial hCG (%) were 0.36% in the spontaneous regression group and 1.45% in the postmolar GTN group in the second week (p=0.003). Prediction of postmolar GTN by hCG regression rate revealed a sensitivity of 48.0% and specificity of 89.5% with a cut-off value of 0.716% and area under the curve (AUC) of 0.759 in the 2nd week (pfactor for postmolar GTN. Crown Copyright © 2011. Published by Elsevier Ireland Ltd. All rights reserved.

  19. Body Mole Map

    Science.gov (United States)

    ... find any new or suspicious spots on your skin, or any spots that are changing, itching or bleeding, make an ... to you by the American Academy of Dermatology’s SPOT Skin Cancer ™ campaign, dedicated to a world without skin ...

  20. Contribuição ao estudo tecnológico da canela-azeitona, Rapanea ferruginea (Ruiz e Pav. Mez, uma espécie pioneira. I - Características anatômicas da madeira.

    Directory of Open Access Journals (Sweden)

    Antônio Lelis Pinheiro

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 São descritos neste trabalho os aspectos anatômicos da madeira de Rapanea ferruginea (Ruiz et Pav. Mez. Apresentam-se dados quantitativos de 11 caracteres do xilema secundário, bem como fotomicrografias das estruturas anatômicas da madeira. Os raios largos e a largos e a baixa percentagem de parênquima axial são os caracteres mais importantes na estrutura do lenho.

  1. Adesão ao tratamento da ambliopia

    OpenAIRE

    Arakaki,Mara Regina; Schellini,Silvana Artioli; Heimbeck,Felipe Jorge; Furuya,Margareth Tiemi; Padovani,Carlos Roberto

    2004-01-01

    OBJETIVO: Avaliar a influência da adesão no resultado do tratamento da ambliopia. MÉTODOS: Foi feito estudo retrospectivo avaliando a resposta ao tratamento da ambliopia em 151 crianças portadoras de ambliopia, tratadas com esquema de oclusão diária, com o número de dias variando de acordo com a idade da criança. Os pacientes foram separados em dois grupos que diferiam entre si pela adesão ou não ao tratamento. RESULTADOS: Observou-se que o tratamento teve melhores resultados nas crianças de ...

  2. High-Performance CCSDS AOS Protocol Implementation in FPGA

    Science.gov (United States)

    Clare, Loren P.; Torgerson, Jordan L.; Pang, Jackson

    2010-01-01

    The Consultative Committee for Space Data Systems (CCSDS) Advanced Orbiting Systems (AOS) space data link protocol provides a framing layer between channel coding such as LDPC (low-density parity-check) and higher-layer link multiplexing protocols such as CCSDS Encapsulation Service, which is described in the following article. Recent advancement in RF modem technology has allowed multi-megabit transmission over space links. With this increase in data rate, the CCSDS AOS protocol implementation needs to be optimized to both reduce energy consumption and operate at a high rate.

  3. A Prediction of the Damping Properties of Hindered Phenol AO-60/polyacrylate Rubber (AO-60/ACM) Composites through Molecular Dynamics Simulation

    Science.gov (United States)

    Yang, Da-Wei; Zhao, Xiu-Ying; Zhang, Geng; Li, Qiang-Guo; Wu, Si-Zhu

    2016-05-01

    Molecule dynamics (MD) simulation, a molecular-level method, was applied to predict the damping properties of AO-60/polyacrylate rubber (AO-60/ACM) composites before experimental measures were performed. MD simulation results revealed that two types of hydrogen bond, namely, type A (AO-60) -OH•••O=C- (ACM), type B (AO-60) - OH•••O=C- (AO-60) were formed. Then, the AO-60/ACM composites were fabricated and tested to verify the accuracy of the MD simulation through dynamic mechanical thermal analysis (DMTA). DMTA results showed that the introduction of AO-60 could remarkably improve the damping properties of the composites, including the increase of glass transition temperature (Tg) alongside with the loss factor (tan δ), also indicating the AO-60/ACM(98/100) had the best damping performance amongst the composites which verified by the experimental.

  4. Adesão ao tratamento da ambliopia

    Directory of Open Access Journals (Sweden)

    Arakaki Mara Regina

    2004-01-01

    Full Text Available OBJETIVO: Avaliar a influência da adesão no resultado do tratamento da ambliopia. MÉTODOS: Foi feito estudo retrospectivo avaliando a resposta ao tratamento da ambliopia em 151 crianças portadoras de ambliopia, tratadas com esquema de oclusão diária, com o número de dias variando de acordo com a idade da criança. Os pacientes foram separados em dois grupos que diferiam entre si pela adesão ou não ao tratamento. RESULTADOS: Observou-se que o tratamento teve melhores resultados nas crianças de 4 a 7 anos e que aderiram ao tratamento; as crianças que aderiram ao tratamento necessitaram de tempo menor de permanência em tratamento. Mesmo crianças com idade superior a 7 anos tiveram resultados positivos com a oclusão. CONCLUSÃO:O tratamento oclusivo é um meio terapêutico eficiente desde que haja a cooperação do paciente.

  5. Resposta ao estresse: II. Resiliência e vulnerabilidade

    Directory of Open Access Journals (Sweden)

    Nicole Leite Galvão-Coelho

    2015-06-01

    Full Text Available ResumoA crescente exposição a estressores na vida cotidiana aumentou significativamente a investigação da resposta ao estresse nas duas últimas décadas. Embora associada a consequências negativas, pois muitas patologias físicas e mentais são desencadeadas por exposição crônica a estressores, esta resposta é indispensável para sobrevivencia do indivíduo e é extremamente adaptativa quando ativada de forma aguda. Na parte I desta revisão foram abordados os conceitos de homestase e alostase e os sistemas fisiológicos ativados durante a resposta ao estresse. Na parte II serão discutidos fatores que modulam a resposta ao estresse tais como sexo, temperamento, períodos críticos do desenvolvimento e a presença ou ausência de suporte social. A interação entre os fatores genéticos e ambientais gera os perfis da resposta psicofisiológica que caracterizam os fenótipos de susceptibilidade e resiliência frente aos estressores e sua relação com uma patologia mental cada vez mais presente na sociedade moderna, o transtorno de estresse pós-traumático.

  6. Polycladida from Curaçao and faunistically related regions

    NARCIS (Netherlands)

    Bois-Reymond Marcus, du Eveline; Marcus, Ernst

    1968-01-01

    Professor Dr. DIVA DINIZ CORRÊA, Head of the Department of Zoology of the University of São Paulo, was able to work at the “Caraïbisch Marien-Biologisch Instituut” (Caribbean Marine Biological Institute: Carmabi) at Curaçao from December 1965 to March 1966, thanks to a grant received from the

  7. Reptiles and Amphibians of Curaçao

    NARCIS (Netherlands)

    Rooij, de Nelly

    1922-01-01

    The Zoological Museum of Amsterdam received some collections of reptiles from Curaçao made by Dr. J. BOEKE in 1905, by Dr. C. J. VAN DER HORST in 1920 and by Mr. G. J. H. MOLENGRAAFF „Engineer” in 1921. I had the opportunity of studying them and will here give an enumeration of all the reptiles

  8. On some Polychaetous Annelids from Curaçao

    NARCIS (Netherlands)

    Horst, R.

    1922-01-01

    Though we know already a rather large number of Polychaetous Annelids from the Caribbean Sea, hitherto, as far as I know, no Annelids have been described from the coast of the island Curaçao and I therefore was very glad, that my colleague Dr. VAN DER HORST kindly placed in my hands for

  9. [Richard C. M. Mole: The Baltic states from the Soviet Union to the European Union. Identity, discourse and power in the post-communist transition of Estonia, Latvia and Lithuania] / Karsten Brüggemann

    Index Scriptorium Estoniae

    Brüggemann, Karsten, 1965-

    2014-01-01

    Arvustus: Mole, Richard C. M. The Baltic States from the Soviet Union to the European Union : identity, discourse and power in the post-communist transition of Estonia, Latvia and Lithuania. London ; New York : Routledge, 2012, 2013

  10. Effect of the Ti/B{sub 4}C mole ratio on the reaction products and reaction mechanism in an Al–Ti–B{sub 4}C powder mixture

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Jingjing [Key Laboratory of Liquid Structure and Heredity of Materials, Ministry of Education, Shandong University, Jinan 250100 (China); Light Metal Division, Korea Institute of Materials Science, Changwon 642-831 (Korea, Republic of); Lee, Jung-Moo, E-mail: jmoolee@kims.re.kr [Light Metal Division, Korea Institute of Materials Science, Changwon 642-831 (Korea, Republic of); Cho, Young-Hee; Kim, Su-Hyeon [Light Metal Division, Korea Institute of Materials Science, Changwon 642-831 (Korea, Republic of); Yu, Huashun [Key Laboratory of Liquid Structure and Heredity of Materials, Ministry of Education, Shandong University, Jinan 250100 (China)

    2014-10-15

    The effect of the Ti/B{sub 4}C mole ratio on the fabrication behavior of Al composites is investigated using Al–Ti–B{sub 4}C powder mixtures as reactants. The quick spontaneous infiltration (QSI) process combined with the combustion reaction and DTA analysis were used. According to the thermodynamic predictions, which are verified in the experimental results, TiB{sub 2} is formed in all the samples whereas TiC is only formed in reactants with a Ti/B{sub 4}C mole ratio of more than two. The C atoms from the reacted B{sub 4}C do not move into TiC but instead they move into Al{sub 3}BC or Al{sub 4}C{sub 3} when the Ti/B{sub 4}C mole ratio is less than two. In addition, the reaction mechanism with a Ti/B{sub 4}C mole ratio of 0.75 is investigated extensively. - Highlights: • The critical role of the Ti/B{sub 4}C mole ratio on the reaction products of Al–Ti–B{sub 4}C was studied using experiments. • The experimental results are also supported by thermodynamic calculations presented in this paper. • The reaction mechanism with a Ti/B{sub 4}C mole ratio of 0.75 is investigated extensively.

  11. Effect of Al mole fraction on structural and electrical properties of AlxGa1-xN/GaN heterostructures grown by plasma-assisted molecular beam epitaxy

    International Nuclear Information System (INIS)

    Hussein, A. SH.; Hassan, Z.; Thahab, S.M.; Ng, S.S.; Hassan, H. Abu; Chin, C.W.

    2011-01-01

    The effect of Al mole fractions on the structural and electrical properties of Al x Ga 1-x N/GaN thin films grown by plasma-assisted molecular beam epitaxy (PA-MBE) on Si (1 1 1) substrates has been investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), and current-voltage (I-V) measurements. X-ray results revealed that the AlGaN/GaN/AlN was epitaxially grown on Si substrate. By applying Vegard's law, the Al mole fractions of Al x Ga 1-x N samples were found to be 0.11, 0.24, 0.30 and 0.43, respectively. The structural and morphology results indicated that there is a relatively larger tensile strain for the sample with the smallest Al mole fraction; while a smaller compressive strain and larger grain size appear with Al mole fraction equal to 0.30. The strain gets relaxed with the highest Al mole fraction sample. Finally, the linear relationship between the barrier height and Al mole fraction was obtained.

  12. Frequência de mola hidatiforme em tecidos obtidos por curetagem uterina Frequency of hydatidiform mole in tissue obtained by curettage

    Directory of Open Access Journals (Sweden)

    Andressa Biscaro

    2012-06-01

    Full Text Available OBJETIVO: Determinar a frequência de mola hidatiforme em tecidos obtidos por curetagem uterina. MÉTODOS: Estudo transversal, prospectivo e descritivo que incluiu pacientes submetidas à curetagem uterina por diagnóstico de aborto ou mola hidatiforme cujo material obtido foi encaminhado para exame anatomopatológico. Foram excluídas aquelas que não aceitaram participar da pesquisa, recusando-se a assinar o Termo de Consentimento Informado Livre e Esclarecido. Foram analisadas as seguintes variáveis: achados anatomopatológicos, idade, raça/cor, número de gestações e abortos prévios, idade gestacional no momento do diagnóstico, níveis séricos quantitativos da fração beta da gonadotrofina coriônica humana e achados ultrassonográficos. As variáveis foram empregadas para a verificação com o diagnóstico histológico, considerado o padrão-ouro. Os dados foram armazenados e analisados no software Microsoft Excel® e no programa Epi-Info, versão 6.0 (STATCALC e os resultados apresentados como frequência (porcentagem ou média±desvio padrão. Para a associação entre variáveis qualitativas foi usado o teste do χ², e admitiu-se significância estatística quando pPURPOSE: To determine the frequency of hydatiform mole in tissues obtained by curettage. METHODS: A cross-sectional, prospective and descriptive conducted on patients who underwent curretage due to a diagnosis of abortion or hydatiform mole whose material was sent for pathological examination. We excluded women who did not accept to participate and refused to sign the free informed consent form. We studied the following variables: pathological findings, age, race, number of pregnancies and previous abortions, gestational age at diagnosis, quantitative serum beta fraction of human chorionic gonadotropin and ultrasound findings. The data were compared to the to histological diagnosis, considered to be the gold standard. Data were stored and analyzed in Microsoft Excel

  13. Tamanho ao nascer e problemas de saúde mental aos 11 anos em uma coorte brasileira de nascimentos

    Directory of Open Access Journals (Sweden)

    Erika Alejandra Giraldo Gallo

    2011-08-01

    Full Text Available O objetivo foi avaliar a associação entre tamanho ao nascer e problemas de saúde mental aos 11 anos na Coorte de Nascimentos de Pelotas, Rio Grande do Sul, Brasil, de 1993. Foram pesados e medidos ao nascer 4.358 recém-nascidos. Avaliou-se problemas de saúde mental com o questionário de capacidades e dificuldades (Strengths and Difficulties Questionnaire - SDQ. A prevalência de problemas de saúde mental foi de 32% (IC95%: 31-33. Na análise ajustada, os 291 (6,7% recém-nascidos com escorez de peso/idade e os 268 (6,2% com índice de massa corporal (IMC/idade +2 DP tiveram, respectivamente, 34% (IC95%: 6-71 e 19% (IC95%: 1-40 maior risco de apresentar esses problemas se comparados com aqueles com escore normal. Os resultados sugerem que fatores ocorridos na gestação e refletidos nas medidas de tamanho ao nascer podem ocasionar problemas de saúde mental em etapas tardias.

  14. Holocene Evolution of Qing'ao Embayment, Southern China

    Science.gov (United States)

    Switzer, A. D.; Yu, F.; Chen, B.; Zheng, Z.; Wang, D.

    2012-12-01

    The Holocene evolution of the Qing'ao embayment, Nan'ao Island, southern China, is primarily the result of the interaction of tectonic activity, climate variation and changes in relative sea level. Characterizing the evolutionary history of the relatively small Qing'ao embayment during the Holocene will help improve our understanding of the driving mechanisms of coastal evolution in the area. To reconstruct the Holocene evolution history we analyzed the grain size, loss on ignition (LOI) and carbonate content of modern and core samples. Modern environmental analogs were examined in surface samples ranging from the coastal sand dunes through to offshore. The results of these modern samples suggest that dune sand (mean size of ~2.33Phi) are slightly finer than beach sand (mean size of 2.13Phi), and nearshore sediment is much coarser than offshore sediment (mean size of 5.90Phi). This modern analogs were then applied to 8 percussion cores from the Qing'ao embayment. A chronological framework obtained from 11 radiocarbon samples suggests that the embayment started to accept deposition since early Holocene, ~8500 cal. yr. BP. Three main phases of Holocene evolution were identified. A basin wide shell-rich sand sheet forms the basal Holocene facies and overlies clay rich presumably Pleistocene sediments or bedrock. This facies records an initial sedimentation phase associated with the early Holocene transgression into the embayment (~8500-6000 cal. yr. BP). The basal facies grades upward to a mixed sandy-mud facies which includes lagoonal clayey-silts, flood tide delta sands and records an estuarine phase lasting from ~6000-1000 cal. yr. BP that appears coincident with falling regional sea levels. Coincident with the estuarine phase is a period of coastal dune building recorded as yet undated massive sands that are found in the upper fill. Toward the end of the estuarine phase it is apparent that dune migration has restricted the lagoon entrance and that this was

  15. Phase behaviour of pseudo-binary systems of pressurized ((propane + L,L-lactide)) at different ethanol to L,L-lactide mole ratios

    International Nuclear Information System (INIS)

    Bender, João P.; Tres, Marcus V.; Corazza, Marcos L.; Ferreira, Sandra R.S.; Oliveira, J. Vladimir

    2014-01-01

    Highlights: • Phase equilibrium data of (propane + L,L-lactide) system at different ethanol to monomer mole ratios. • Static synthetic method from (323 to 353) K and pressures up to 3.3 MPa. • (Vapour + liquid) (VLE) was observed with bubble point (BP) type transitions. • Experimental modelled using the Peng–Robinson (PR) equation with the Wong–Sandler (PR–WS) rule. - Abstract: This work reports phase equilibrium data of pressurized (propane + L,L-lactide) system at different ethanol to monomer mole ratios (9:1; 7:1; 5:1). Phase equilibrium experiments were accomplished in a high-pressure variable-volume view cell employing the static synthetic method. (Vapour + liquid) equilibrium data for the pseudo-binary systems were determined within the temperature range from (323 to 353) K and pressures up to 3.3 MPa. For the systems investigated, (vapour + liquid) equilibrium (VLE) was visually recorded. It was observed that an increase in temperature or in propane concentration led to a pronounced rise in pressure transition values. On the other hand, an increase in the ethanol to L,L-lactide mole ratio led to a reduction in pressure transitions, whereas a reduction in ethanol concentration complicates the achievement of one-phase homogeneous system. Thus, rapid complete miscibility of the system can be controlled by the amount of ethanol added as a co-solvent. The experimental results were modelled using the Peng–Robinson (PR) equation of state with the Wong–Sandler (PR–WS) mixing rule, providing a good representation of the experimental phase transition points

  16. Structural Changes and Lack of HCN1 Channels in the Binaural Auditory Brainstem of the Naked Mole-Rat (Heterocephalus glaber).

    Science.gov (United States)

    Gessele, Nikodemus; Garcia-Pino, Elisabet; Omerbašić, Damir; Park, Thomas J; Koch, Ursula

    2016-01-01

    Naked mole-rats (Heterocephalus glaber) live in large eu-social, underground colonies in narrow burrows and are exposed to a large repertoire of communication signals but negligible binaural sound localization cues, such as interaural time and intensity differences. We therefore asked whether monaural and binaural auditory brainstem nuclei in the naked mole-rat are differentially adjusted to this acoustic environment. Using antibody stainings against excitatory and inhibitory presynaptic structures, namely the vesicular glutamate transporter VGluT1 and the glycine transporter GlyT2 we identified all major auditory brainstem nuclei except the superior paraolivary nucleus in these animals. Naked mole-rats possess a well structured medial superior olive, with a similar synaptic arrangement to interaural-time-difference encoding animals. The neighboring lateral superior olive, which analyzes interaural intensity differences, is large and elongated, whereas the medial nucleus of the trapezoid body, which provides the contralateral inhibitory input to these binaural nuclei, is reduced in size. In contrast, the cochlear nucleus, the nuclei of the lateral lemniscus and the inferior colliculus are not considerably different when compared to other rodent species. Most interestingly, binaural auditory brainstem nuclei lack the membrane-bound hyperpolarization-activated channel HCN1, a voltage-gated ion channel that greatly contributes to the fast integration times in binaural nuclei of the superior olivary complex in other species. This suggests substantially lengthened membrane time constants and thus prolonged temporal integration of inputs in binaural auditory brainstem neurons and might be linked to the severely degenerated sound localization abilities in these animals.

  17. Structural Changes and Lack of HCN1 Channels in the Binaural Auditory Brainstem of the Naked Mole-Rat (Heterocephalus glaber.

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    Nikodemus Gessele

    Full Text Available Naked mole-rats (Heterocephalus glaber live in large eu-social, underground colonies in narrow burrows and are exposed to a large repertoire of communication signals but negligible binaural sound localization cues, such as interaural time and intensity differences. We therefore asked whether monaural and binaural auditory brainstem nuclei in the naked mole-rat are differentially adjusted to this acoustic environment. Using antibody stainings against excitatory and inhibitory presynaptic structures, namely the vesicular glutamate transporter VGluT1 and the glycine transporter GlyT2 we identified all major auditory brainstem nuclei except the superior paraolivary nucleus in these animals. Naked mole-rats possess a well structured medial superior olive, with a similar synaptic arrangement to interaural-time-difference encoding animals. The neighboring lateral superior olive, which analyzes interaural intensity differences, is large and elongated, whereas the medial nucleus of the trapezoid body, which provides the contralateral inhibitory input to these binaural nuclei, is reduced in size. In contrast, the cochlear nucleus, the nuclei of the lateral lemniscus and the inferior colliculus are not considerably different when compared to other rodent species. Most interestingly, binaural auditory brainstem nuclei lack the membrane-bound hyperpolarization-activated channel HCN1, a voltage-gated ion channel that greatly contributes to the fast integration times in binaural nuclei of the superior olivary complex in other species. This suggests substantially lengthened membrane time constants and thus prolonged temporal integration of inputs in binaural auditory brainstem neurons and might be linked to the severely degenerated sound localization abilities in these animals.

  18. Intelligent vibration control of ELTs and large AO hardware

    Science.gov (United States)

    Pott, J.-U.; Kürster, M.; Trowitzsch, J.; Borelli, J.; Rohloff, R.-R.; Herbst, T.; Böhm, M.; Keck, A.; Ruppel, T.; Sawodny, O.

    2012-07-01

    MPIA leads the construction of the LINC-NIRVANA instrument, the MCAO-supported Fizeau imager for the LBT, serves as pathfinder for future ELT-AO imagers in terms of size and technology. In this contribution, we review recent results and significant progress made on the development of key items of our stratgey to achieve a piston stability of up to 100nm during a science exposure. We present an overview of our vibration control strategies for optical path and tip-tilt stabilization, involving accelerometer based real-time vibration measurements, vibration sensitive active control of actuators, and the development of a dynamical model of the LBT. MPIA also co-develops the E-ELT first-light NIR imager MICADO (both SCAO and MCAO assisted). Our experiences, made with LINC-NIRVANA, will be fed into the MICADO structural AO design to reach highest on-sky sensitivity.

  19. Magnetic MOF for AO7 Removal and Targeted Delivery

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    Xue-Qing Zhan

    2018-06-01

    Full Text Available Owing to their high surface area and porosity, metal-organic frameworks (MOFs have been gradually employed for a myriad of applications ranging from sensing, pollutant adsorption, and drug delivery to environmental remediation and catalysis. Magnetic nanoparticles-metal-organic frameworks (MMOFs hybrid materials can facilitate facile removal of MOFs from solutions. In this report, we report the synthesis of Fe3O4@UiO-66 by encapsulation and simulated the drug loading and release by studying the adsorption and release of AO7. Thus, we loaded these MMOFs with AO7 and found that they were able to trigger and control its release by simply applying an external magnetic field. The magnetic field heats the magnets in the MOF, which causes the load to burst from the framework.

  20. Morphogenetic Effects of Resettlement of Mole Voles (Ellobius talpinus Pall., 1770) from the Southern Population to the Northern Boundary of the Species Range.

    Science.gov (United States)

    Vasil'ev, A G; Bol'shakov, V N; Vasil'eva, I A; Evdokimov, N G; Sineva, N V

    2018-01-01

    Geometric morphometry has been used to reveal transformations of mandible morphogenesis in the offspring of mole voles resettled to the northern part of the species range from a southern population. The transformations were new compared to both the original (southern) and the aboriginal (northern) populations. A significant increase in the intragroup morphological disparity estimated by the mean nearest neighbor distance (MNND) in the resettled animals compared to both aboriginal populations is an indirect indication of an increased developmental instability in the resettled animals exposed to new climatic conditions.

  1. Diagnostic reproducibility of hydatidiform moles: ancillary techniques (p57 immunohistochemistry and molecular genotyping) improve morphologic diagnosis for both recently trained and experienced gynecologic pathologists.

    Science.gov (United States)

    Gupta, Mamta; Vang, Russell; Yemelyanova, Anna V; Kurman, Robert J; Li, Fanghong Rose; Maambo, Emily C; Murphy, Kathleen M; DeScipio, Cheryl; Thompson, Carol B; Ronnett, Brigitte M

    2012-12-01

    Distinction of hydatidiform moles from nonmolar specimens (NMs) and subclassification of hydatidiform moles as complete hydatidiform mole (CHM) and partial hydatidiform mole (PHM) are important for clinical practice and investigational studies; however, diagnosis based solely on morphology is affected by interobserver variability. Molecular genotyping can distinguish these entities by discerning androgenetic diploidy, diandric triploidy, and biparental diploidy to diagnose CHMs, PHMs, and NMs, respectively. Eighty genotyped cases (27 CHMs, 27 PHMs, 26 NMs) were selected from a series of 200 potentially molar specimens previously diagnosed using p57 immunohistochemistry and genotyping. Cases were classified by 6 pathologists (3 faculty level gynecologic pathologists and 3 fellows) on the basis of morphology, masked to p57 immunostaining and genotyping results, into 1 of 3 categories (CHM, PHM, or NM) during 2 diagnostic rounds; a third round incorporating p57 immunostaining results was also conducted. Consensus diagnoses (those rendered by 2 of 3 pathologists in each group) were also determined. Performance of experienced gynecologic pathologists versus fellow pathologists was compared, using genotyping results as the gold standard. Correct classification of CHMs ranged from 59% to 100%; there were no statistically significant differences in performance of faculty versus fellows in any round (P-values of 0.13, 0.67, and 0.54 for rounds 1 to 3, respectively). Correct classification of PHMs ranged from 26% to 93%, with statistically significantly better performance of faculty versus fellows in each round (P-values of 0.04, <0.01, and <0.01 for rounds 1 to 3, respectively). Correct classification of NMs ranged from 31% to 92%, with statistically significantly better performance of faculty only in round 2 (P-values of 1.0, <0.01, and 0.61 for rounds 1 to 3, respectively). Correct classification of all cases combined ranged from 51% to 75% by morphology and 70% to 80

  2. Mujer de 39 a??os con cefalea intensa

    OpenAIRE

    Santiago-D??az, Carlos; Zamora-Pasadas, M??nica; S??nchez-Bern??, Isabel; Jim??nez-Alonso, Juan

    2012-01-01

    Paciente de 39 a??os que acude a urgencias por presentar un cuadro de cefalea intensa, holocraneal, con foto y sonofobia intensas, acompa??ada de febr??cula. En la exploraci??n f??sica ??nicamente destacaban leves roncus en base de hemit??rax izquierdo. Las pruebas complementarias iniciales fueron normales. Tras un estudio exhaustivo y fundamentalmente una insistencia en la anamnesis por ??rganos y aparatos, finalmente se lleg?? al diagn??stico.

  3. Da esclerodermia ao síndrome de poems

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    Ana Sofia Montez

    2015-12-01

    Full Text Available Homem, 84 anos, com antecedentes de hipotiroidismo e alterações cutâneas recentes compatíveis com esclerodermia, confirmadas em biópsia cutânea, mas com estudo auto-imune negativo. Foi internado por queixas constitucionais. Ao exame objetivo, apresentava-se emagrecido, com lesões de vitiligo nas mãos além das de esclerodermia (figura 1 e distensão abdominal com ascite

  4. Da esclerodermia ao síndrome de poems

    OpenAIRE

    Ana Sofia Montez; Elsa Rocha

    2015-01-01

    Homem, 84 anos, com antecedentes de hipotiroidismo e alterações cutâneas recentes compatíveis com esclerodermia, confirmadas em biópsia cutânea, mas com estudo auto-imune negativo. Foi internado por queixas constitucionais. Ao exame objetivo, apresentava-se emagrecido, com lesões de vitiligo nas mãos além das de esclerodermia (figura 1) e distensão abdominal com ascite

  5. Cultivo de Spirulina maxima ao ar livre - I. Inverno

    Directory of Open Access Journals (Sweden)

    Rogério Lacaz Ruiz

    1990-12-01

    e noturno indicaram que o melhor período de produção de biomassa durante o dia foi das 8 - 13 h e a produtividade atingiu valor máximo de 8,08 g/metro quadrado/dia. Durante todo o experimento foi necessário adicionar água ao sistema, devido à ausência de chuvas; e o total acrescentado equivaleu a 72,14 mm de chuva.

  6. Um crime delicado: conversando aos infinitos - "Um retrato de cavalo"

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    Cid Ottoni Bylaardt

    2010-12-01

    um cavalo e seu retrato, a partir dos sentimentos contraditórios de seu dono, que considera um crime aquele clique. Temos aí um retrato, imagem subtraída ilicitamente ao seu dono: escrita, representação. Temos também uma narrativa, escrita do retrato, representação da representação. Este artigo pretende mostrar como Guimarães Rosa manipula as ambiguidades da linguagem literária, levando-a além dos limites da representação, explorando seu fascínio, seu saber, que não é da ordem da compreensão. O cavalo e seu retrato fazem refletir sobre a literatura, sobre seu excesso de verdade que compõe sua mentira. Ao escrever o cavalo e seu retrato, o texto não consegue fixar nenhuma verdade, apenas imagens fugidias que compõem recapítulos: há sempre um escrito a se sobrepor a outro, sem determinar onde está o verdadeiro, onde está o que o nega, a remeter o olhar ao reino da fascinação, onde a imagem perde o valor de significação para se tornar pura paixão da indeterminação, da indiferença.

  7. AO Distal Radius Fracture Classification: Global Perspective on Observer Agreement

    Science.gov (United States)

    Jayakumar, Prakash; Teunis, Teun; Giménez, Beatriz Bravo; Verstreken, Frederik; Di Mascio, Livio; Jupiter, Jesse B.

    2016-01-01

    Background The primary objective of this study was to test interobserver reliability when classifying fractures by consensus by AO types and groups among a large international group of surgeons. Secondarily, we assessed the difference in inter- and intraobserver agreement of the AO classification in relation to geographical location, level of training, and subspecialty. Methods A randomized set of radiographic and computed tomographic images from a consecutive series of 96 distal radius fractures (DRFs), treated between October 2010 and April 2013, was classified using an electronic web-based portal by an invited group of participants on two occasions. Results Interobserver reliability was substantial when classifying AO type A fractures but fair and moderate for type B and C fractures, respectively. No difference was observed by location, except for an apparent difference between participants from India and Australia classifying type B fractures. No statistically significant associations were observed comparing interobserver agreement by level of training and no differences were shown comparing subspecialties. Intra-rater reproducibility was “substantial” for fracture types and “fair” for fracture groups with no difference accounting for location, training level, or specialty. Conclusion Improved definition of reliability and reproducibility of this classification may be achieved using large international groups of raters, empowering decision making on which system to utilize. Level of Evidence Level III PMID:28119795

  8. Barreiras ao tratamento da hipertensão arterial

    Directory of Open Access Journals (Sweden)

    Maria Vilani Cavalcante Guedes

    2011-12-01

    Full Text Available Objetivou-se descrever as barreiras encontradas pelas pessoas portadoras de hipertensão arterial para a não adesão ao tratamento e controle dos níveis de sua pressão arterial. Estudo descritivo e transversal realizado em seis unidades básicas de saúde em Fortaleza-Ceará, Brasil, com 246 pessoas inscritas no Programa de Controle de Hipertensão Arterial há, no mínimo, um ano. Coletaram-se dados com entrevista estruturada e no prontuário eletrônico. Identificaram-se 69 pesquisados com níveis pressóricos normalizados. As principais barreiras apontadas foram: baixas condições financeiras, tratamento contínuo com muitos remédios e prática de atividade física. Concluiu-se que as barreiras ao tratamento anti-hipertensivo compreendem a pessoa adoecida, seu ambiente de vida e acesso aos cuidados de saúde.

  9. Resistências aos Antibióticos em Pneumologia

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    J.G. Saraiva da Cunha

    1999-01-01

    Full Text Available RESUMO: A crescente resistência dos microrganismos aos agentes antimicrobianos tem motivado o interesse da comunidade científica com o objectivo de avaliar a dimensão do problema, perceber a sua origem e encontrar eveotuais soluções. São quatro os mecanismos basicos de resistência das bácterias aos antibióticos: inactivação enzimática, alteração ou excesso de produção das molécules alvo, diminuição da permeabilidade da parede celulalr e eliminação actia (eflauxo do antibiótico para o exterior da bactéria. Para cada uma das diferentes classes de antibioticos sio referidos os priocipais mecanismos de resisteocia, assim como se exemplifica quais os germes de particular relevância em pneumologia que maiores dificuldades terapêuticas actualmente colocam. Finalmente enumeram-se algumas medidas para contrariar a expansão do problema. Palavras-Chave: Resistências aos antibióticos, Pneumologia, Pneumococo

  10. O Primeiro Ano do Grupo de Apoio ao Primeiranista

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    Rodolfo Furlan Damiano

    Full Text Available Este trabalho é um relato de experiência do primeiro ano de funcionamento do Grupo de Apoio ao Primeiranista (GAP. O GAP é um grupo desenvolvido por alunos de Medicina de uma universidade privada do Estado de São Paulo, apoiado por sua direção, com o intuito de amparar e humanizar a recepção aos ingressantes. O início das atividades do GAP ocorreu em 2013 com base nos conceitos de tutoria/mentoring, aplicado por outras escolas médicas brasileiras. Nas reuniões semanais do GAP, os participantes (alunos do primeiro ano e mentores (acadêmicos veteranos e uma psicóloga institucional discutiam temas como resiliência, compaixão, humildade e respeito. Durante as reuniões, notou-se uma problemática extensa em relação ao trote universitário, referido pelos primeiranistas como o pior problema enfrentado na universidade. Na sequência dos encontros, o grupo direcionou suas ações para reduzir o trote na universidade. O GAP, apesar de estar em seu primeiro ano de funcionamento, mostrou ter eficácia relevante, podendo ser um modelo a ser implementado em outras universidades.

  11. Fatores associados a baixa adesao ao tratamento medicamentoso em idosos

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    Noemia Urruth Leao Tavares

    2013-12-01

    Full Text Available OBJETIVO : Analisar fatores associados à baixa adesão ao tratamento medicamentoso em idosos. MÉTODOS : Estudo transversal de base populacional, com amostra representativa de 1.593 indivíduos com 60 anos ou mais, residentes na região urbana de Bagé, RS, em 2008. A amostragem foi realizada em múltiplos estágios. Os dados foram coletados em entrevistas individuais nos domicílios. Analisou-se a associação entre a baixa adesão referida ao tratamento medicamentoso mensurado pelo Brief Medication Questionnaire (BMQ e fatores demográficos, socioeconômicos, comportamentais e de saúde, assistência e prescrição. Foi utilizado modelo de regressão de Poisson para estimar as razões de prevalência bruta e ajustada, os respectivos intervalos de confiança de 95% e p-valor (teste de Wald. RESULTADOS : Cerca de 78,0% dos indivíduos referiram ter usado algum medicamento nos sete dias precedentes à entrevista. Desses, cerca de 1 / 3 foram considerados com baixa adesão ao tratamento. Os fatores significativamente associados à baixa adesão foram: idade (65 a 74 anos, não ter plano de saúde, ter que comprar (totalmente ou em parte os seus medicamentos, ter três ou mais morbidades, possuir incapacidade instrumental para a vida diária e usar três ou mais medicamentos. CONCLUSÕES : A utilização elevada de medicamentos, decorrente da alta prevalência de doenças crônico-degenerativas em idosos, e o acesso ao tratamento devem ser considerados pelos profissionais de saúde para adoção de estratégias que visem diminuir a baixa adesão ao tratamento, aumentando a resolutividade terapêutica e a qualidade de vida desses pacientes.

  12. SIMS chemical and isotopic analysis of impact features from LDEF experiments AO187-1 and AO187-2

    Science.gov (United States)

    Stadermann, Frank J.; Amari, Sachiko; Foote, John; Swan, Pat; Walker, Robert M.; Zinner, Ernst

    1995-01-01

    Previous secondary ion mass spectrometry (SIMS) studies of extended impact features from LDEF capture cell experiment AO187-2 showed that it is possible to distinguish natural and man-made particle impacts based on the chemical composition of projectile residues. The same measurement technique has now been applied to specially prepared gold target impacts from experiment AO187-1 in order to identify the origins of projectiles that left deposits too thin to be analyzed by conventional energy-dispersive x-ray (EDX) spectroscopy. The results indicate that SIMS may be the method of choice for the analysis of impact deposits on a variety of sample surfaces. SIMS was also used to determine the isotopic compositions of impact residues from several natural projectiles. Within the precision of the measurements all analyzed residues show isotopically normal compositions.

  13. Suppression of Plant Immune Responses by the Pseudomonas savastanoi pv. savastanoi NCPPB 3335 Type III Effector Tyrosine Phosphatases HopAO1 and HopAO2

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    María Pilar Castañeda-Ojeda

    2017-05-01

    Full Text Available The effector repertoire of the olive pathogen P. savastanoi pv. savastanoi NCPPB 3335 includes two members of the HopAO effector family, one of the most diverse T3E families of the P. syringae complex. The study described here explores the phylogeny of these dissimilar members, HopAO1 and HopAO2, among the complex and reveals their activities as immune defense suppressors. Although HopAO1 is predominantly encoded by phylogroup 3 strains isolated from woody organs of woody hosts, both HopAO1 and HopAO2 are phylogenetically clustered according to the woody/herbaceous nature of their host of isolation, suggesting host specialization of the HopAO family across the P. syringae complex. HopAO1 and HopAO2 translocate into plant cells and show hrpL-dependent expression, which allows their classification as actively deployed type III effectors. Our data also show that HopAO1 and HopAO2 possess phosphatase activity, a hallmark of the members of this family. Both of them exert an inhibitory effect on early plant defense responses, such as ROS production and callose deposition, and are able to suppress ETI responses induced by the effectorless polymutant of P. syringae pv. tomato DC3000 (DC3000D28E in Nicotiana. Moreover, we demonstrate that a ΔhopAO1 mutant of P. savastanoi NCPBB 3335 exhibits a reduced fitness and virulence in olive plants, which supports the relevance of this effector during the interaction of this strain with its host plants. This work contributes to the field with the first report regarding functional analysis of HopAO homologs encoded by P. syringae or P. savastanoi strains isolated from woody hosts.

  14. Suppression of Plant Immune Responses by the Pseudomonas savastanoi pv. savastanoi NCPPB 3335 Type III Effector Tyrosine Phosphatases HopAO1 and HopAO2

    Science.gov (United States)

    Castañeda-Ojeda, María Pilar; Moreno-Pérez, Alba; Ramos, Cayo; López-Solanilla, Emilia

    2017-01-01

    The effector repertoire of the olive pathogen P. savastanoi pv. savastanoi NCPPB 3335 includes two members of the HopAO effector family, one of the most diverse T3E families of the P. syringae complex. The study described here explores the phylogeny of these dissimilar members, HopAO1 and HopAO2, among the complex and reveals their activities as immune defense suppressors. Although HopAO1 is predominantly encoded by phylogroup 3 strains isolated from woody organs of woody hosts, both HopAO1 and HopAO2 are phylogenetically clustered according to the woody/herbaceous nature of their host of isolation, suggesting host specialization of the HopAO family across the P. syringae complex. HopAO1 and HopAO2 translocate into plant cells and show hrpL-dependent expression, which allows their classification as actively deployed type III effectors. Our data also show that HopAO1 and HopAO2 possess phosphatase activity, a hallmark of the members of this family. Both of them exert an inhibitory effect on early plant defense responses, such as ROS production and callose deposition, and are able to suppress ETI responses induced by the effectorless polymutant of P. syringae pv. tomato DC3000 (DC3000D28E) in Nicotiana. Moreover, we demonstrate that a ΔhopAO1 mutant of P. savastanoi NCPBB 3335 exhibits a reduced fitness and virulence in olive plants, which supports the relevance of this effector during the interaction of this strain with its host plants. This work contributes to the field with the first report regarding functional analysis of HopAO homologs encoded by P. syringae or P. savastanoi strains isolated from woody hosts. PMID:28529516

  15. Suporte relacionado ao cuidado em saúde ao doente com aids: o modelo de comboio e a enfermagem

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    Edilene Aparecida Araújo da Silveira

    2011-06-01

    Full Text Available O presente estudo teve como objetivo conhecer a relação e a troca de suporte entre o doente com AIDS e pessoas à sua volta. O estudo está baseado no modelo de comboio das relações sociais que tem como uma de suas características a representação da rede em três círculos concêntricos. Os participantes eram pessoas que estavam internadas e que falaram sobre o suporte relacionado ao cuidado em saúde proporcionado por pessoas próximas, ou seja, que pertenciam ao círculo interno do comboio. A maioria dos participantes descreveu o círculo como sendo composto por no máximo cinco integrantes, sendo que pessoas da família foram as mais citadas. Os profissionais de saúde precisam conhecer o paciente e seu comboio, reconhecendo-os no contexto psicossocial e cultural de forma a favorecer a aceitação da soropositividade, mudanças no estilo de vida, ajuda nos cuidados de saúde e adesão ao tratamento.

  16. Reproduction is associated with a tissue-dependent reduction of oxidative stress in eusocial female Damaraland mole-rats (Fukomys damarensis.

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    Christina M Schmidt

    Full Text Available Oxidative stress has been implicated as both a physiological cost of reproduction and a driving force on an animal's lifespan. Since increased reproductive effort is generally linked with a reduction in survival, it has been proposed that oxidative stress may influence this relationship. Support for this hypothesis is inconsistent, but this may, in part, be due to the type of tissues that have been analyzed. In Damaraland mole-rats the sole reproducing female in the colony is also the longest lived. Therefore, if oxidative stress does impact the trade-off between reproduction and survival in general, this species may possess some form of enhanced defense. We assessed this relationship by comparing markers of oxidative damage (malondialdehyde, MDA; protein carbonyls, PC and antioxidants (total antioxidant capacity, TAC; superoxide dismutase, SOD in various tissues including plasma, erythrocytes, heart, liver, kidney and skeletal muscle between wild-caught reproductive and non-reproductive female Damaraland mole-rats. Reproductive females exhibited significantly lower levels of PC across all tissues, and lower levels of MDA in heart, kidney and liver relative to non-reproductive females. Levels of TAC and SOD did not differ significantly according to reproductive state. The reduction in oxidative damage in breeding females may be attributable to the unusual social structure of this species, as similar relationships have been observed between reproductive and non-reproductive eusocial insects.

  17. Effects of KOH:ZnCl2 mole ratio on the phase formation, morphological and inhibitive properties of potassium zinc phosphate (PZP) pigments

    International Nuclear Information System (INIS)

    Askari, F.; Ghasemi, E.; Ramezanzadeh, B.; Mahdavian, M.

    2015-01-01

    Graphical abstract: This figure illustrates the inhibition mechanism. The film precipitation on the anodic and cathodic regions of the metal surface causes a drop in dissolution rate of steel. - Highlights: • We synthesized the ZP pigments via coprecipitation method. • Effect of KOH:ZnCl 2 on phase formation, morphology and inhibitive performance of pigment. • KOH:ZnCl 2 ratio is effective on phase formation and morphology of ZP pigments. • KZn 2 H(PO 4 ) 2 phase showed superior corrosion inhibition behavior than KZnPO 4 . - Abstract: Different types of potassium zinc phosphate (PZP) pigments were synthesized by a co-precipitation method. The PZPs were obtained through changing KOH:ZnCl 2 mole ratio. The chemical composition of the pigments was studied by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM). Then, the extracts of the PZPs were prepared in 3.5% (w/w) NaCl solution. The inhibitive performance of the pigments was investigated by polarization technique and SEM surface analysis through dipping mild steel panels in the pigment extracts for different immersion times. Results showed that changing ZnCl 2 /KOH mole ratio could influence formation of KZn 2 H(PO 4 ) 2 and KZnPO 4 phases. It was known that the KZn 2 H(PO 4 ) 2 phase has a superior corrosion inhibition behavior in 3.5% NaCl solution than KZnPO 4 phase

  18. How attractive is the girl next door? An assessment of spatial mate acquisition and paternity in the solitary Cape dune mole-rat, Bathyergus suillus.

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    Timothy C Bray

    Full Text Available Behavioural observations of reproduction and mate choice in wild fossorial rodents are extremely limited and consequently indirect methods are typically used to infer mating strategies. We use a combination of morphological, reproductive, spatial, and genetic data to investigate the reproductive strategy of a solitary endemic species, the Cape dune mole-rat Bathyergus suillus. These data provide the first account on the population dynamics of this species. Marked sexual dimorphism was apparent with males being both significantly larger and heavier than females. Of all females sampled 36% had previously reproduced and 12% were pregnant at the time of capture. Post-partum sex ratio was found to be significantly skewed in favour of females. The paternity of fifteen litters (n = 37 was calculated, with sires assigned to progeny using both categorical and full probability methods, and including a distance function. The maximum distance between progeny and a putative sire was determined as 2149 m with males moving between sub-populations. We suggest that above-ground movement should not be ignored in the consideration of mate acquisition behaviour of subterranean mammals. Estimated levels of multiple paternity were shown to be potentially as high as 26%, as determined using sibship and sire assignment methods. Such high levels of multiple paternity have not been found in other solitary mole-rat species. The data therefore suggest polyandry with no evidence as yet for polygyny.

  19. Familial Melanoma Associated with Li-Fraumeni Syndrome and Atypical Mole Syndrome: Total-body Digital Photography, Dermoscopy and Confocal Microscopy.

    Science.gov (United States)

    Giavedoni, Priscila; Ririe, Marnie; Carrera, Cristina; Puig, Susana; Malvehy, Josep

    2017-06-09

    Li-Fraumeni syndrome (LFS) is a rare autosomal dominant disorder caused by a mutation in the p53 gene. Melanoma is considered to be a rare, controversial component of LFS. The aim of this study is to describe the utility of systematic screening for melanoma in patients with LFS and atypical mole syndrome. Two 28-year-old identical twin sisters with LFS and atypical moles were monitored by physical examination, total-body digital photography and dermoscopy be-tween 2006 and 2014. A total of 117, predominantly dark-brown, reticular naevi were identified on case 1 and 105 on case 2. Excisions were performed during the evaluation period of 1 in-situ melanoma and 3 basal cell carcinomas in case 1, and 1 in-situ melanoma and 1 early invasive melanoma in case 2. The remaining melanocytic lesions in both patients were stable during follow-up. The 3 melanomas were new atypical lesions detected with total-body photography and dermoscopy. In conclusion, monitoring LFS patients with total-body photography and dermoscopy may be useful to detect early melanoma.

  20. Gestação gemelar de mola hidatiforme completa com feto vivo Twin pregnancy with complete hydatidiform mole and living fetus

    Directory of Open Access Journals (Sweden)

    Daniela Angerame Yela

    2011-04-01

    Full Text Available A gestação gemelar de uma mola hidatiforme completa coexistindo com um feto vivo é uma entidade rara. Os poucos casos descritos na literatura mostram que, em geral, esse tipo de gestação cursa com risco aumentado de aborto espontâneo, parto prematuro, morte fetal intraútero, sangramento, pré-eclâmpsia e doença trofoblástica persistente. Neste artigo, descreveremos o caso de uma primigesta de 20 anos de idade que apresentou uma gestação gemelar de feto vivo com mola completa, a qual transcorreu sem intercorrências e complicações e cujo diagnóstico diferencial com degeneração da placenta foi feito com anatomopatológico.Twin pregnancy with a complete hydatidiform mole and a living fetus is a rare entity. According to the few cases described in the literature, this kind of gestation commonly has higher risks of miscarriage, preterm delivery, intrauterine fetal death, bleeding, preeclampsia and persistent trophoblastic disease. This study reports the case of a 20 years old primiparous patient with complete hydatidiform mole and living fetus, whose pregnancy was successfully carried to term. The differential diagnosis with placental degeneration was reached through anatomopathological investigation.

  1. Effect of Cu{sup 2+}/Al{sup 3+} mole ratio on structure of Cu-Al bimetallic nanoparticles prepared by radiation induced method

    Energy Technology Data Exchange (ETDEWEB)

    Abedini, Alam; Larki, Farhad; Saion, Elias; Noroozi, Monir [Putra Malaysia Univ., Serdang, Selangor (Malaysia). Dept. of Physics

    2013-07-15

    Cu-Al bimetallic nanoparticles were synthesized by gamma irradiation technique in aqueous solutions containing metal chlorides as precursors, polyvinyl alcohol (PVA) as a capping agent, isopropanol as a radical scavenger, and distilled water as a solvent. The Cu-Al bimetallic nanoparticles were characterized by transmission electron microscopy (TEM), UV-visible absorption spectrometry, powder X-ray diffractometer (XRD), and Energy-dispersive X-ray spectroscopy (EDX). The TEM, XRD, EDX, and absorption analyses confirmed the formation of core-shell structure of Cu-Al bimetallic nanoparticles at lower Cu{sup 2+}/Al{sup 3+} mole ratio, and the formation of Cu-Al alloy nanoparticles at higher Cu{sup 2+}/Al{sup 3+} mole ratio. The TEM analysis for particle size and size distribution revealed that the average particle size of Cu-Al bimetallic nanoparticles decreased with the increase of absorbed dose. It may be explained due to the competition between nucleation and aggregation processes in the formation of metallic nanoparticles under irradiation. (orig.)

  2. Development of Mole Concept Module Based on Structured Inquiry with Interconection of Macro, Submicro, and Symbolic Representation for Grade X of Senior High School

    Science.gov (United States)

    Sagita, R.; Azra, F.; Azhar, M.

    2018-04-01

    The research has created the module of mole concept based on structured inquiry with interconection of macro, submicro, and symbolic representation and determined the validity and practicality of the module. The research type was Research and Development (R&D). The development model was 4-D models that consist of four steps: define, design, develop, and disseminate. The research was limited on develop step. The instrument of the research was questionnaire form that consist of validity and practicality sheets. The module was validated by 5 validators. Practicality module was tested by 2 chemistry teachers and 28 students of grade XI MIA 5 at SMAN 4 of Padang. Validity and practicality data were analysed by using the kappa Cohen formula. The moment kappa average of 5 validators was 0,95 with highest validity category. The moment kappa average of teachers and students were 0,89 and 0,91 praticality with high category. The result of the research showed that the module of mole concept based on structured inquiry with interconection of macro, submicro, and symbolic representation was valid and practice to be used on the learning chemistry.

  3. Green FLASH: energy efficient real-time control for AO

    Science.gov (United States)

    Gratadour, D.; Dipper, N.; Biasi, R.; Deneux, H.; Bernard, J.; Brule, J.; Dembet, R.; Doucet, N.; Ferreira, F.; Gendron, E.; Laine, M.; Perret, D.; Rousset, G.; Sevin, A.; Bitenc, U.; Geng, D.; Younger, E.; Andrighettoni, M.; Angerer, G.; Patauner, C.; Pescoller, D.; Porta, F.; Dufourcq, G.; Flaischer, A.; Leclere, J.-B.; Nai, A.; Palazzari, P.; Pretet, D.; Rouaud, C.

    2016-07-01

    The main goal of Green Flash is to design and build a prototype for a Real-Time Controller (RTC) targeting the European Extremely Large Telescope (E-ELT) Adaptive Optics (AO) instrumentation. The E-ELT is a 39m diameter telescope to see first light in the early 2020s. To build this critical component of the telescope operations, the astronomical community is facing technical challenges, emerging from the combination of high data transfer bandwidth, low latency and high throughput requirements, similar to the identified critical barriers on the road to Exascale. With Green Flash, we will propose technical solutions, assess these enabling technologies through prototyping and assemble a full scale demonstrator to be validated with a simulator and tested on sky. With this R&D program we aim at feeding the E-ELT AO systems preliminary design studies, led by the selected first-light instruments consortia, with technological validations supporting the designs of their RTC modules. Our strategy is based on a strong interaction between academic and industrial partners. Components specifications and system requirements are derived from the AO application. Industrial partners lead the development of enabling technologies aiming at innovative tailored solutions with potential wide application range. The academic partners provide the missing links in the ecosystem, targeting their application with mainstream solutions. This increases both the value and market opportunities of the developed products. A prototype harboring all the features is used to assess the performance. It also provides the proof of concept for a resilient modular solution to equip a large scale European scientific facility, while containing the development cost by providing opportunities for return on investment.

  4. Introdución ao laboratorio químico

    OpenAIRE

    Rumbo Gómez, Antonio

    2010-01-01

    A materia inclúese no primeiro ano de estudo da titulación de Enxeñería Técnica Industrial especialidade de Química Industrial. Impártese no primeiro cuadrimestre e é o primeiro contacto que ten o alumnado co laboratorio de química, polo que se considera de indubidable interese o estudo de certos conceptos básicos desta area. Os conceptos desta unidade didáctica serán aplicables a todos os laboratorios que o alumnado terá que realizar ao longo da titulación noutras materias práctic...

  5. Fatores associados ao excesso de peso entre mulheres

    OpenAIRE

    Lopes,Aline Cristine Souza; Reyes,Alessandra Nunes Lima; Menezes,Mariana Carvalho de; Santos,Luana Caroline dos; César,Cibele Comini

    2012-01-01

    Objetivou-se caracterizar as usuárias e identificar os fatores associados ao excesso de peso em serviço público de Promoção à Saúde (SPS). Estudo transversal com todas as mulheres de SPS, denominado Academia da Cidade. Utilizaram-se variáveis antropométricas, consumo e hábitos alimentares, e relato de morbidades. Realizou-se análise descritiva e regressão linear múltipla (p

  6. Atenção ao Suicídio

    Directory of Open Access Journals (Sweden)

    Berenice Rheinreimer

    2015-11-01

    Full Text Available De acordo com a OMS a cada ano ocorrem 1 milhão de mortes causadas por suicídio, número maior que a soma das mortes causadas por guerras e homicídios (1. Até 2030, as mortes por suicídio podem chegar a 1 milhão e quinhentos mil, caso sejam mantidos os índices de crescimento atuais (2, 3. Para cada morte, ocorrem de 10 a 40 tentativas de suicídio, ou seja, 10 a 40 milhões de tentativas de suicídio ao ano (4.

  7. Do ensinar ao aprender: o passaporte para o futuro

    OpenAIRE

    Almeida, Ana Rita Conceição

    2016-01-01

    O presente Relatório Final é apresentado no âmbito da conclusão do Mestrado de Educação Pré-Escolar e Ensino do 1.º Ciclo do Ensino Básico. Este documento reflete o caminho percorrido pelas aprendizagens adquiridas e reflexões proporcionadas ao longo dos dois estágios que constituem este Mestrado, sendo que um decorreu em Educação Pré-Escolar e o outro no 1.º Ciclo do Ensino Básico. Este documento divide-se em duas grandes partes estruturantes que constituem o pilar de todo este trabalho. Na ...

  8. Aspect-Oriented Business Process Modeling with AO4BPMN

    Science.gov (United States)

    Charfi, Anis; Müller, Heiko; Mezini, Mira

    Many crosscutting concerns in business processes need to be addressed already at the business process modeling level such as compliance, auditing, billing, and separation of duties. However, existing business process modeling languages including OMG's Business Process Modeling Notation (BPMN) lack appropriate means for expressing such concerns in a modular way. In this paper, we motivate the need for aspect-oriented concepts in business process modeling languages and propose an aspect-oriented extension to BPMN called AO4BPMN. We also present a graphical editor supporting that extension.

  9. Contabilidade gerencial como apoio ao "just in time"

    OpenAIRE

    Costa, Sérgio De Carvalho

    2010-01-01

    Descreve as técnicas da filosofia "Just in Time" e apresenta as modificações necessárias aos Sistemas Gerencias para que se tornem coerentes com a filosofia JIT, bem como meçam adequadamente a melhoria contínua da Empresa. Critica tanto o Custeio' Direto como o Custeio por Absorção, apresentando como alternativa o Custeio "ABC". Mostra como o JIT facilita o Custeio dos Produtos e destaca os aspectos estratégicos da Avaliação de Desempenho. Finalmente discorre sobre um estudo de...

  10. Checklist of the birds of Aruba, Curaçao and Bonaire, South Caribbean

    NARCIS (Netherlands)

    Prins, T.G.; Reuter, J.H.; Debrot, A.O.; Wattel, J.; Nijman, V.

    2009-01-01

    We present an updated checklist of the birds of the islands of Aruba, Curaçao and Bonaire, and the islets of Klein Curaçao and Klein Bonaire, southern Caribbean, and compare this with earlier checklists (K.H. Voous, Stud. Fauna Curaçao Carib. Isl. 7: 1-260, 1957; Ardea 53: 205-234, 1965; Birds of

  11. Clinical significance of measurement of changes of serum IL-2, SIL-2R levels, B lymphocyte number and T-cell subsets after chemotherapy in patients with malignant hydatidiform mole

    International Nuclear Information System (INIS)

    Zhang Guangcai

    2007-01-01

    Objective: To study the clinical significance of changes of serum IL-2, SIL-2R level, peripheral blood B lymphocyte number and T-cell subsets after chemotherapy in patients with malignant hydatidiform mole. Methods: Serum IL-2 ( with RIA), SIL-2R level (with ELISA) and peripheral blood B lymphocytes number as well as T subsets (with monoclonal antibody technique) were measured both before and after chemotherapy in 32 patients with malignant hydatidiform mole as well as in 35 controls. Results: Before chemotherapy serum SIL-2R level and B lymphocyte were significantly higher in the patients than those in controls (P<0.01), while the serum IL-2 level, CD3, CD4, CD4/CD8 were significantly lower (P<0.01). Six months after chemotherapy the levels changed markedly toward normal, but remained significantly different from those in controls (P<0.05). Conclusion: Abnormal immuno-regulation were present in patients with malignant mole. (authors)

  12. O SENTIMENTO DO PACIENTE FRENTE AO CANCELAMENTO DA CIRURGIA

    Directory of Open Access Journals (Sweden)

    Fernanda Aparecida Silva

    2017-02-01

    Full Text Available O paciente, frente ao cancelamento da cirurgia, pode apresentar sentimentos: tristeza, agressividade, revolta, e perder a confiança da equipe cirúrgica. Objetivo: Identificar o sentimento do paciente internado frente ao cancelamento de sua cirurgia. Método: Pesquisa descritiva e quali-quantitativa. A coleta de dados ocorreu após aprovação do Comitê de Ética sob o n0 45/11, com 20 pacientes internados em um hospital do vale do Paraíba paulista. Resultados: Predominou:80% do sexo masculino, 30% de 51 a 60 anos. Principal causa cancelamento: 35% material, 10% vaga na Unidade de Terapia Intensiva (UTI, e 55% médicos, exames, vaga no centro cirúrgico ou infecção. Os sentimentos foram agrupados em quatro categorias: tristeza; estresse; conformismo e sentimentos variados.Conclusão: O perfil predominante dos pacientes foi de homens, entre 51 a 60 anos. A principal causa do cancelamento foi a falta de material; o sentimento de maior destaque foi a tristeza.

  13. Implante Coclear: estudos concernentes à biopolítica, ao biopoder e ao biocapital em III volumes

    OpenAIRE

    Garcia, Eduardo de Campos

    2015-01-01

    Esta tese, embora aparentemente trate de assuntos concernentes ao Implante Coclear, seu objeto esbarra numa análise do que de modo visuoimperceptual autoriza a operacionalidade das modificações corporais que implicam do imbricamento entre homem e máquina: pela criação de linguagem clínico discursiva, na ordem jurídico-biológica, nas tramas interpessoais e pela própria pessoa que se autodeclara ciborgue de ouvido biônico e que acaba envolvida pela ideia da formação de outras identidades e de u...

  14. OBSOLESCÊNCIA PROGRAMADA E TEORIA DO DECRESCIMENTO VERSUS DIREITO AO DESENVOLVIMENTO E AO CONSUMO (SUSTENTÁVEIS)

    OpenAIRE

    Oliveira da Silva, Maria Beatriz Beatriz; UNiversidade Federal de Santa Maria (UFSM)

    2012-01-01

    Inspirado em um documentário intitulado “Prêt à Jeter”, este texto tem como objetivo central colocar em apreciação algumas reflexões acerca da obsolescência programada como uma das estratégias do modelo de desenvolvimento capitalista e ensaiar uma reposta às seguintes questões: para combater a obsolescência programada se faz necessária a defesa de uma “teoria do decrescimento”? Sendo a obsolescência programada uma das estratégias da sociedade de consumo, é possível combatê-la e, ao mesmo temp...

  15. Antimicrobial resistance profile of Staphylococcus aureus isolates obtained from skin and soft tissue infections of outpatients from a university hospital in Recife -PE, Brazil Perfil de resistência antimicrobiana de isolados de Staphylococcus aureus provenientes de infecções de pele e tecidos moles de pacientes ambulatoriais de um hospital universitário em Recife - PE, Brasil

    Directory of Open Access Journals (Sweden)

    Fabiana Beserra Caraciolo

    2012-12-01

    tornando importante também fora do ambiente hospitalar, particularmente nos Estados Unidos. No Brasil, desde 2005, têm sido relatados casos de infecções cutâneas comunitárias causadas por MRSA, porém estudos de resistência envolvendo pacientes ambulatoriais são escassos. OBJETIVO: Conhecer o perfil de resistência de S. aureus envolvidos em infecções de pele e partes moles de pacientes atendidos no ambulatório de Dermatologia de um hospital universitário de Recife, Pernambuco. MÉTODO: Estudo prospectivo envolvendo 30 pacientes com infecções de pele e tecidos moles atendidos no ambulatório de Dermatologia de maio a novembro de 2011. Para avaliação da suscetibilidade dos S. aureus aos antibióticos foram utilizados teste de disco-difusão e placa de screening de oxacilina. RESULTADOS: Das 30 amostras analisadas, 19 (63% tiveram cultura positiva para S. aureus. Os seguintes padrões de resistência dos S. aureus foram observados: penicilina, 95%; tetraciclina, 32%; eritromicina, 21%; gentamicina, 16%; cefoxitina, 11%; oxacilina, 11%; sulfametoxazol-trimetoprima, 11%; clorafenicol, 11%; clindamicina, 5%; e ciprofloxacina, 0%. Um dos MRSA identificados foi obtido de paciente sem fatores de risco para sua aquisição, e além de aos betalactâmicos, mostrou-se resistente apenas à tetraciclina. CONCLUSÕES: Em relação aos padrões de resistência dos S. aureus, destacaram-se as resistências à tetraciclina, eritromicina e gentamicina. Documentou-se, pela primeira vez em Pernambuco, um caso de infecção cutânea causada por MRSA associado à comunidade.

  16. Alternativas de controle pós-colheita da podridão-parda e da podridão-mole em frutos de pessegueiro Alternatives for post-harvest control of brown rot and soft rot in peach fruits

    Directory of Open Access Journals (Sweden)

    Vicente Luiz de Carvalho

    2009-03-01

    Full Text Available As exigências do consumidor são cada vez maiores com relação à qualidade de produtos in natura. As podridões, além de causar perdas na produção, reduzem a qualidade final do fruto, interferindo significativamente na comercialização. Objetivou-se, com este trabalho, avaliar a eficiência de produtos alternativos, aplicados na pós-colheita, no controle da podridão-parda (Monilinia fructicola e da podridão-mole (Rhizopus spp. em pêssegos. O experimento foi conduzido no município de Nepomuceno-MG, em talhão de pessegueiro da cultivar Diamante, com 10 anos de idade. O delineamento experimental utilizado foi o inteiramente casualizado, com 7 tratamentos e 3 repetições. Para o estudo, foram selecionados frutos sem lesões e sem sintomas das doenças. Os frutos foram mergulhados por 30 segundos em solução contendo os seguintes tratamentos: 1-Óleo de cravo 0,01%; 2-Dióxido de cloro 0,1%; 3-Dióxido de cloro 0,05%; 4-Cloreto de benzalcônio 0,25%; 5-Dicloran 0,12%; 6-Iprodione 0,15% e 7-Testemunha (somente água. Após os tratamentos, os frutos, em número de 10, foram colocados em bandejas esterilizadas, em três repetições. O experimento foi conduzido em condições de ambiente não controlado, sendo feitas avaliações do desenvolvimento das doenças aos 3 e aos 5 dias após os tratamentos em 2005 e aos 3; 6 e aos 9 dias após o tratamento em 2006. O iprodione controlou a incidência e a severidade de M. fructicola e Rhizopus spp. O dicloran foi o tratamento mais eficiente para o controle do Rhizopus spp. e intermediário para M. fructicola. Os tratamentos com óleo de cravo e dióxido de cloro, na maior dose, reduziram a incidência de Rhizopus spp. e para severidade apresentaram resultados intermediários.The consumers demand are increasing with regard to the quality of in nature products. The rot, besides causing loss in production, reduce the quality of the product, interfering significantly in the marketing. The objective

  17. Quantification of urban atmospheric boundary layer greenhouse gas dry mole fraction enhancements in the dormant season: Results from the Indianapolis Flux Experiment (INFLUX

    Directory of Open Access Journals (Sweden)

    Natasha L. Miles

    2017-06-01

    Full Text Available We assess the detectability of city emissions via a tower-based greenhouse gas (GHG network, as part of the Indianapolis Flux (INFLUX experiment. By examining afternoon-averaged results from a network of carbon dioxide (CO2, methane (CH4, and carbon monoxide (CO mole fraction measurements in Indianapolis, Indiana for 2011–2013, we quantify spatial and temporal patterns in urban atmospheric GHG dry mole fractions. The platform for these measurements is twelve communications towers spread across the metropolitan region, ranging in height from 39 to 136 m above ground level, and instrumented with cavity ring-down spectrometers. Nine of the sites were deployed as of January 2013 and data from these sites are the focus of this paper. A background site, chosen such that it is on the predominantly upwind side of the city, is utilized to quantify enhancements caused by urban emissions. Afternoon averaged mole fractions are studied because this is the time of day during which the height of the boundary layer is most steady in time and the area that influences the tower measurements is likely to be largest. Additionally, atmospheric transport models have better performance in simulating the daytime convective boundary layer compared to the nighttime boundary layer. Averaged from January through April of 2013, the mean urban dormant-season enhancements range from 0.3 ppm CO2 at the site 24 km typically downwind of the edge of the city (Site 09 to 1.4 ppm at the site at the downwind edge of the city (Site 02 to 2.9 ppm at the downtown site (Site 03. When the wind is aligned such that the sites are downwind of the urban area, the enhancements are increased, to 1.6 ppm at Site 09, and 3.3 ppm at Site 02. Differences in sampling height affect the reported urban enhancement by up to 50%, but the overall spatial pattern remains similar. The time interval over which the afternoon data are averaged alters the calculated urban enhancement by an average of 0.4 ppm

  18. Reaching Out to Send a Message: Proteins Associated with Neurite Outgrowth and Neurotransmission are Altered with Age in the Long-Lived Naked Mole-Rat.

    Science.gov (United States)

    Triplett, Judy C; Swomley, Aaron M; Kirk, Jessime; Grimes, Kelly M; Lewis, Kaitilyn N; Orr, Miranda E; Rodriguez, Karl A; Cai, Jian; Klein, Jon B; Buffenstein, Rochelle; Butterfield, D Allan

    2016-07-01

    Aging is the greatest risk factor for developing neurodegenerative diseases, which are associated with diminished neurotransmission as well as neuronal structure and function. However, several traits seemingly evolved to avert or delay age-related deterioration in the brain of the longest-lived rodent, the naked mole-rat (NMR). The NMR remarkably also exhibits negligible senescence, maintaining an extended healthspan for ~75 % of its life span. Using a proteomic approach, statistically significant changes with age in expression and/or phosphorylation levels of proteins associated with neurite outgrowth and neurotransmission were identified in the brain of the NMR and include: cofilin-1; collapsin response mediator protein 2; actin depolymerizing factor; spectrin alpha chain; septin-7; syntaxin-binding protein 1; synapsin-2 isoform IIB; and dynamin 1. We hypothesize that such changes may contribute to the extended lifespan and healthspan of the NMR.

  19. Correlation analysis of reactivity in the oxidation of methionine by benzimidazolium fluorochromate in different mole fractions of acetic acid–water mixture

    Directory of Open Access Journals (Sweden)

    S. Sheik Mansoor

    2016-09-01

    Full Text Available The kinetics of oxidation of methionine (Met by benzimidazolium fluorochromate (BIFC has been studied in the presence of chloroacetic acid. The reaction is first order with respect to methionine, BIFC and acid. The reaction rate has been determined at different temperatures and activation parameters calculated. With an increase in the mole fraction of acetic acid in its aqueous mixture, the rate increases. The solvent effect has been analyzed using the Kamlet’s multi parametric equation. A correlation of data with the Kamlet–Taft solvatochromic parameters (α, β, π∗ suggests that the specific solute–solvent interactions play a major role in governing the reactivity. The reaction does not induce polymerization of acrylonitrile. A suitable mechanism has been proposed.

  20. Avaliação das alterações em tecidos moles e duros de pacientes submetidos à cirurgia ortognática combinada utilizando cefalometria computadorizada

    OpenAIRE

    Becker, Otávio Emmel

    2012-01-01

    OBJETIVO: Avaliar as diferenças e verificar a correlação existente entre pontos de tecido mole e pontos de tecido duro do perfil facial e entre medidas em vias aéreas faríngeas e os movimentos nos ossos gnáticos, nos períodos pré operatório e pós operatórios, a curto e médio prazo, em pacientes com padrão facial Classe III, submetidos à cirurgia ortognática (CO) bimaxilar (combinada-avanço de maxila e recuo de mandíbula).MATERIAL E METODOS: Tele radiografias pré-operatórias (T1), pós-operatór...

  1. NEGRITUDE E UNIVERSIDADE: EVIDENCIANDO QUESTÕES RELACIONADAS AO INGRESSO E AOS PROJETOS CURRICULARES. IOLANDA OLIVEIRA (ORGANIZADORA, 2015

    Directory of Open Access Journals (Sweden)

    Joselina da Silva

    2017-04-01

    Full Text Available A década de setenta marca um dos momentos inauguradores, no Brasil, da constituição do corpo como bandeira e afirmação identitária por parte dos movimentos sociais negros. A negritude, em performance inspirada nos movimentos brasileiros dos anos trinta, em São Paulo, tomou a rua nas diversas capitais do país. Eram os movimentos Black Soul e os blocos afros. Os primeiros, aludiam diretamente aos afro estadunidenses; os segundos, por sua vez, cunhados por uma juventude cultural afro-baiana, instituíam uma moda capilar advinda dos trançados de diferentes países do continente africano, somados aos dreadlocks jamaicanos rematados com as roupas trajadas pelos orixás do panteão yorubá, das casas de culto ancestral. Nessa ambiência é que se destaca o protagonismo da intelectual que organiza o livro Negritude e universidade.

  2. Assessing Animal Welfare Impacts in the Management of European Rabbits (Oryctolagus cuniculus), European Moles (Talpa europaea) and Carrion Crows (Corvus corone)

    Science.gov (United States)

    Baker, Sandra E.; Sharp, Trudy M.; Macdonald, David W.

    2016-01-01

    Human-wildlife conflict is a global issue. Attempts to manage this conflict impact upon wild animal welfare, an issue receiving little attention until relatively recently. Where human activities harm animal welfare these effects should be minimised where possible. However, little is known about the welfare impacts of different wildlife management interventions, and opinions on impacts vary widely. Welfare impacts therefore need to be assessed objectively. Our objectives were to: 1) establish whether an existing welfare assessment model could differentiate and rank the impacts of different wildlife management interventions (for decision-making purposes); 2) identify and evaluate any additional benefits of making formal welfare assessments; and 3) illustrate issues raised by application of the model. We applied the welfare assessment model to interventions commonly used with rabbits (Oryctolagus cuniculus), moles (Talpa europaea) and crows (Corvus corone) in the UK. The model ranked interventions for rabbits (least impact first: fencing, head shot, chest shot) and crows (shooting, scaring, live trapping with cervical dislocation). For moles, managing molehills and tunnels scored least impact. Both spring trapping, and live trapping followed by translocation, scored greater impacts, but these could not be compared directly as they scored on different axes of the model. Some rankings appeared counter-intuitive, highlighting the need for objective formal welfare assessments. As well as ranking the humaneness of interventions, the model highlighted future research needs and how Standard Operating Procedures might be improved. The model is a milestone in assessing wildlife management welfare impacts, but our research revealed some limitations of the model and we discuss likely challenges in resolving these. In future, the model might be developed to improve its utility, e.g. by refining the time-scales. It might also be used to reach consensus among stakeholders about

  3. Assessing Animal Welfare Impacts in the Management of European Rabbits (Oryctolagus cuniculus), European Moles (Talpa europaea) and Carrion Crows (Corvus corone).

    Science.gov (United States)

    Baker, Sandra E; Sharp, Trudy M; Macdonald, David W

    2016-01-01

    Human-wildlife conflict is a global issue. Attempts to manage this conflict impact upon wild animal welfare, an issue receiving little attention until relatively recently. Where human activities harm animal welfare these effects should be minimised where possible. However, little is known about the welfare impacts of different wildlife management interventions, and opinions on impacts vary widely. Welfare impacts therefore need to be assessed objectively. Our objectives were to: 1) establish whether an existing welfare assessment model could differentiate and rank the impacts of different wildlife management interventions (for decision-making purposes); 2) identify and evaluate any additional benefits of making formal welfare assessments; and 3) illustrate issues raised by application of the model. We applied the welfare assessment model to interventions commonly used with rabbits (Oryctolagus cuniculus), moles (Talpa europaea) and crows (Corvus corone) in the UK. The model ranked interventions for rabbits (least impact first: fencing, head shot, chest shot) and crows (shooting, scaring, live trapping with cervical dislocation). For moles, managing molehills and tunnels scored least impact. Both spring trapping, and live trapping followed by translocation, scored greater impacts, but these could not be compared directly as they scored on different axes of the model. Some rankings appeared counter-intuitive, highlighting the need for objective formal welfare assessments. As well as ranking the humaneness of interventions, the model highlighted future research needs and how Standard Operating Procedures might be improved. The model is a milestone in assessing wildlife management welfare impacts, but our research revealed some limitations of the model and we discuss likely challenges in resolving these. In future, the model might be developed to improve its utility, e.g. by refining the time-scales. It might also be used to reach consensus among stakeholders about

  4. Extended postnatal brain development in the longest-lived rodent: prolonged maintenance of neotenous traits in the naked mole-rat brain

    Directory of Open Access Journals (Sweden)

    Miranda E. Orr

    2016-11-01

    Full Text Available The naked mole-rat (NMR is the longest-lived rodent with a maximum lifespan >31 years. Intriguingly, fully-grown naked mole-rats (NMRs exhibit many traits typical of neonatal rodents. However, little is known about NMR growth and maturation, and we question whether sustained neotenous features when compared to mice, reflect an extended developmental period, commensurate with their exceptionally long life. We tracked development from birth to three years of age in the slowest maturing organ, the brain, by measuring mass, neural stem cell proliferation, axonal and dendritic maturation, synaptogenesis and myelination. NMR brain maturation was compared to data from similar sized rodents, mice, and to that of long-lived mammals, humans and non-human primates. We found that at birth, NMR brains are significantly more developed than mice, and rather are more similar to those of newborn primates, with clearly laminated hippocampi and myelinated white matter tracts. Despite this more mature brain at birth than mice, postnatal NMR brain maturation occurs at a far slower rate than mice, taking four-times longer than required for mice to fully complete brain development. At four months of age, NMR brains reach 90% of adult size with stable neuronal cytostructural protein expression whereas myelin protein expression does not plateau until nine months of age in NMRs, and synaptic protein expression continues to change throughout the first three years of life. Intriguingly, NMR axonal composition is more similar to humans than mice whereby NMRs maintain expression of three-repeat (3R tau even after brain growth is complete; mice experience an abrupt downregulation of 3R tau by postnatal day 8 which continues to diminish through six weeks of age. We have identified key ages in NMR cerebral development and suggest that the long-lived NMR may provide neurobiologists an exceptional model to study brain developmental processes that are compressed in common short

  5. Karyotypic evolution and organization of the highly repetitive DNA sequences in the Japanese shrew-moles, Dymecodon pilirostris and Urotrichus talpoides.

    Science.gov (United States)

    Nakata, A; Yoshimura, A; Kuro-o, M; Obara, Y

    2005-01-01

    The karyological relationship and organization of highly repetitive DNA sequences in Japanese shrew-moles were studied by zoo-blot hybridization and fluorescence in situ hybridization (FISH). When the genomic DNA of the eastern race of Urotrichus talpoides was digested with PstI, three fragments of highly repetitive DNA sequences, approximately 0.7, 0.9, and 1.4 kb in length, were observed as distinct bands. The results of FISH in the eastern race of U. talpoides using these three fragments separately as probes showed that the 0.7-kb PstI fragment was distributed in the centromeric regions of most chromosomes, and that the 0.9- and 1.4-kb fragments were predominantly located in the C-heterochromatin region of chromosome 13p. Although the western race of U. talpoides also had three PstI fragments, 0.9- and 1.4-kb PstI fragments were more ambiguous than those of the eastern race. The PstI- digested genomic DNA in Dymecodonpilirostris produced only a faint 0.9-kb band, and its signal patterns obtained by zoo-blot hybridization were clearly different from those of U. talpoides. The 0.7-kb fragment of U. talpoides hybridized strongly with the 0.9-kb fragment of D. pilirostris. In a FISH analysis, the 0.9-kb fragment of D. pilirostris hybridized with highly repetitive DNA in the centromeric regions of most chromosomes from both D. pilirostris and U. talpoides. Zoo-blot hybridization and FISH analyses suggest that the 0.9- and 1.4-kb PstI fragments were generated specifically in the genome of U. talpoides after the common ancestor differentiated into two extant shrew-mole species. A difference in the length of the centromeric elements between U. talpoides and D. pilirostris might be observed due to certain modifications of the repeating unit.

  6. Assessing Animal Welfare Impacts in the Management of European Rabbits (Oryctolagus cuniculus, European Moles (Talpa europaea and Carrion Crows (Corvus corone.

    Directory of Open Access Journals (Sweden)

    Sandra E Baker

    Full Text Available Human-wildlife conflict is a global issue. Attempts to manage this conflict impact upon wild animal welfare, an issue receiving little attention until relatively recently. Where human activities harm animal welfare these effects should be minimised where possible. However, little is known about the welfare impacts of different wildlife management interventions, and opinions on impacts vary widely. Welfare impacts therefore need to be assessed objectively. Our objectives were to: 1 establish whether an existing welfare assessment model could differentiate and rank the impacts of different wildlife management interventions (for decision-making purposes; 2 identify and evaluate any additional benefits of making formal welfare assessments; and 3 illustrate issues raised by application of the model. We applied the welfare assessment model to interventions commonly used with rabbits (Oryctolagus cuniculus, moles (Talpa europaea and crows (Corvus corone in the UK. The model ranked interventions for rabbits (least impact first: fencing, head shot, chest shot and crows (shooting, scaring, live trapping with cervical dislocation. For moles, managing molehills and tunnels scored least impact. Both spring trapping, and live trapping followed by translocation, scored greater impacts, but these could not be compared directly as they scored on different axes of the model. Some rankings appeared counter-intuitive, highlighting the need for objective formal welfare assessments. As well as ranking the humaneness of interventions, the model highlighted future research needs and how Standard Operating Procedures might be improved. The model is a milestone in assessing wildlife management welfare impacts, but our research revealed some limitations of the model and we discuss likely challenges in resolving these. In future, the model might be developed to improve its utility, e.g. by refining the time-scales. It might also be used to reach consensus among

  7. Extended Postnatal Brain Development in the Longest-Lived Rodent: Prolonged Maintenance of Neotenous Traits in the Naked Mole-Rat Brain.

    Science.gov (United States)

    Orr, Miranda E; Garbarino, Valentina R; Salinas, Angelica; Buffenstein, Rochelle

    2016-01-01

    The naked mole-rat (NMR) is the longest-lived rodent with a maximum lifespan >31 years. Intriguingly, fully-grown naked mole-rats (NMRs) exhibit many traits typical of neonatal rodents. However, little is known about NMR growth and maturation, and we question whether sustained neotenous features when compared to mice, reflect an extended developmental period, commensurate with their exceptionally long life. We tracked development from birth to 3 years of age in the slowest maturing organ, the brain, by measuring mass, neural stem cell proliferation, axonal, and dendritic maturation, synaptogenesis and myelination. NMR brain maturation was compared to data from similar sized rodents, mice, and to that of long-lived mammals, humans, and non-human primates. We found that at birth, NMR brains are significantly more developed than mice, and rather are more similar to those of newborn primates, with clearly laminated hippocampi and myelinated white matter tracts. Despite this more mature brain at birth than mice, postnatal NMR brain maturation occurs at a far slower rate than mice, taking four-times longer than required for mice to fully complete brain development. At 4 months of age, NMR brains reach 90% of adult size with stable neuronal cytostructural protein expression whereas myelin protein expression does not plateau until 9 months of age in NMRs, and synaptic protein expression continues to change throughout the first 3 years of life. Intriguingly, NMR axonal composition is more similar to humans than mice whereby NMRs maintain expression of three-repeat (3R) tau even after brain growth is complete; mice experience an abrupt downregulation of 3R tau by postnatal day 8 which continues to diminish through 6 weeks of age. We have identified key ages in NMR cerebral development and suggest that the long-lived NMR may provide neurobiologists an exceptional model to study brain developmental processes that are compressed in common short-lived laboratory animal models.

  8. Effects of KOH:ZnCl{sub 2} mole ratio on the phase formation, morphological and inhibitive properties of potassium zinc phosphate (PZP) pigments

    Energy Technology Data Exchange (ETDEWEB)

    Askari, F. [Inorganic Pigment and Glazes Department, Institute for Color Science and Technology, Tehran 1668814811 (Iran, Islamic Republic of); Surface Coatings and Corrosion Department, Institute for Color Science and Technology, Tehran 1668814811 (Iran, Islamic Republic of); Ghasemi, E., E-mail: eghasemi@iust.ac.ir [Inorganic Pigment and Glazes Department, Institute for Color Science and Technology, Tehran 1668814811 (Iran, Islamic Republic of); Ramezanzadeh, B.; Mahdavian, M. [Surface Coatings and Corrosion Department, Institute for Color Science and Technology, Tehran 1668814811 (Iran, Islamic Republic of)

    2015-05-15

    Graphical abstract: This figure illustrates the inhibition mechanism. The film precipitation on the anodic and cathodic regions of the metal surface causes a drop in dissolution rate of steel. - Highlights: • We synthesized the ZP pigments via coprecipitation method. • Effect of KOH:ZnCl{sub 2} on phase formation, morphology and inhibitive performance of pigment. • KOH:ZnCl{sub 2} ratio is effective on phase formation and morphology of ZP pigments. • KZn{sub 2}H(PO{sub 4}){sub 2} phase showed superior corrosion inhibition behavior than KZnPO{sub 4}. - Abstract: Different types of potassium zinc phosphate (PZP) pigments were synthesized by a co-precipitation method. The PZPs were obtained through changing KOH:ZnCl{sub 2} mole ratio. The chemical composition of the pigments was studied by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM). Then, the extracts of the PZPs were prepared in 3.5% (w/w) NaCl solution. The inhibitive performance of the pigments was investigated by polarization technique and SEM surface analysis through dipping mild steel panels in the pigment extracts for different immersion times. Results showed that changing ZnCl{sub 2}/KOH mole ratio could influence formation of KZn{sub 2}H(PO{sub 4}){sub 2} and KZnPO{sub 4} phases. It was known that the KZn{sub 2}H(PO{sub 4}){sub 2} phase has a superior corrosion inhibition behavior in 3.5% NaCl solution than KZnPO{sub 4} phase.

  9. Polarization burst in the BL Lac object AO 0235 + 164

    Energy Technology Data Exchange (ETDEWEB)

    Impey, C D; Brand, P W.J.L. [Edinburgh Univ. (UK). Dept. of Astronomy; Tapia, S [Steward Observatory, Tucson, AZ (USA)

    1982-01-01

    Simultaneous infrared and optical polarimetry and photometry have been obtained for AO 0235 + 164 covering a five night period. The object underwent a polarization burst during which the 2.2 ..mu..m polarization rose from 17.5 to 28.7 per cent and fell again to 14.9 per cent. At its peak the degree of optical polarization was 43.9 per cent, the highest linear polarization observed in a BL Lac object. The data show the degree of polarization to increase towards shorter wavelengths, and the effect is inconsistent with either dilution by a galactic component or simple one-component synchrotron models. The large changes in polarization are not accompanied by large changes in flux, a result which is difficult to explain using conventional models of these objects. Other implications of the luminosity, polarization and variability are discussed.

  10. MODERNIDADE E LIBERTAÇÃO: DOIS DESAFIOS AO CRISTIANISMO

    Directory of Open Access Journals (Sweden)

    . Editorial

    1987-01-01

    Full Text Available A recente Assembléia Geral da Sociedade de Teologia e Ciências da Religião (SOTER, reunida em Belo Horizonte de 6 a 9 de julho passado, dedicou boa parte de seus trabalhos ao "debate em torno da Modernidade em relação com as preocupações da Teologia da Libertação no Brasil". Está mais uma vez relançada a sempre conflitiva questão da relação entre Cristianismo e Modernidade, só que agora percebida num Continente que não simplesmente goza de algumas luzes da Modernidade, como também sofre efeitos perversos deste processo histórico.

  11. Respostas de pupunheiras (Bactris gasipaes Kunth) jovens ao alagamento

    OpenAIRE

    Carvalho Cláudio José Reis de; Ishida Françoise Yoko

    2002-01-01

    O objetivo deste trabalho foi estudar os efeitos do encharcamento do solo sobre a condutância estomática, o conteúdo relativo de água, o teor de clorofila e a concentração de N, P, K e de açúcares solúveis nos tecidos das folhas, bulbos e raízes de pupunheiras (Bactris gasipaes Kunth) jovens. Plantas com seis meses de idade foram submetidas ao alagamento das raízes por períodos contínuos de sete, 14 e 21 dias. O alagamento induziu o fechamento dos estômatos, apesar de os tecidos das folhas te...

  12. SOCIALISMO E TEOLOGIA FACE AOS ACONTECIMENTOS DO LESTE EUROPEU

    Directory of Open Access Journals (Sweden)

    . Editorial

    1990-01-01

    Full Text Available Com os fatos que se atropelaram no Leste europeu, determinada realização histórica do socialismo — a leninista-estalinista — chegou ao fim. As repercussões deste fato atingem o mundo inteiro, desde a própria Rússia, o centro do sistema, até as longínquas periferias do socialismo chamado ''real", em Cuba e Nicarágua, e também, de modo paradoxal, a África do Sul, onde o desmantelamento do socialismo soviético diminuiu o medo dos afrikaanders brancos diante do movimento antiapartheid, suspeito de ser um posto avançado do comunismo mundial.

  13. Resistência carnavalesca ao espetáculo global

    OpenAIRE

    M. Boje, David

    2002-01-01

    Este artigo apresenta uma proposta de aplicação crítica pós-moderna do "espetáculo" de Debord e do "carnaval" de Bakhtin às encenações que têm acontecido nas ruas das cidades, nos campi de universidades e na Internet resistindo à nova economia globalizada. Na última década, a Administração Pública passou por uma virada pós-moderna, ficando presa às encenações conflitantes de um espetáculo corporativo orquestrado pela mídia e ao carnaval de resistência do discurso de globalização. Pretende-se ...

  14. Cirurgia conservadora, radioterapia externa e reforço de dose com braquiterapia de alta taxa de dose: uma nova perspectiva no tratamento de sarcomas de partes moles do adulto Limb-sparing surgery, external beam radiotherapy and boost with high-dose rate brachytherapy: a new perspective for the treatment of soft tissue sarcomas in adults

    Directory of Open Access Journals (Sweden)

    Antonio Cássio Assis Pellizzon

    2002-03-01

    Full Text Available OBJETIVO: Avaliar a influência no controle local de pacientes adultos e portadores de sarcoma de partes moles em extremidades e submetidos a cirurgia conservadora do membro, com braquiterapia de alta taxa de dose (BATD como reforço para a radioterapia externa (RT. MATERIAL E MÉTODOS: Foram avaliados 16 pacientes tratados, de 1993 até 1999. A RT foi utilizada com finalidade pré ou pós-operatória (30--55 Gy e BATD com dose de 18--36 Gy (fx 3--6 Gy BID. Com base no modelo linear quadrático calculou-se a dose efetiva biológica ("biological effective dose" - BED para o tumor e comparou-se seu valor a dados da literatura internacional, que utiliza tratamentos com RT e braquiterapia de baixa taxa de dose (BBTD. RESULTADOS: Os valores médios e medianos da BED para os sarcomas de partes moles foram de 78,5 Gy7 e 80 Gy7. A análise univariada mostrou que a BED para o tumor, quando utilizada BATD, era semelhante ao valor de 83 Gy7 quando utilizada BBTD (p = 0,008. As taxas de controle local, sobrevida livre de doença e sobrevida global atuarial em cinco anos foram de 83,2%, 75% e 93,7%, respectivamente. CONCLUSÕES: A BATD, quando utilizada como método complementar no reforço de dose da RT no tratamento conservador dos sarcomas de partes moles, apresenta taxas de controle local equiparáveis às da literatura internacional; no entanto, estudos com número maior de pacientes e período maior de seguimento são ainda necessários para determinar o verdadeiro potencial da BATD em substituir a BBTD.PURPOSE: To evaluate the influence on local control in adult patients with soft tissue sarcomas of the limbs that underwent limb-sparing surgery and high-dose rate brachytherapy (HDRB in association with teletherapy. MATERIAL AND METHODS: Sixteen patients treated from 1993 to 1999 were reviewed. Teletherapy was used pre- or postoperatively (30--55 Gy in association with HDRB in a dose range of 18--36 Gy (fx 3--6 Gy BID. The linear quadratic model was

  15. Uso de antibióticos e leveduras para controle da podridão-mole em couve-chinesa Use of antibiotics and yeasts for controlling Chinese cabbage soft rot

    Directory of Open Access Journals (Sweden)

    MRF Mello

    2011-03-01

    Full Text Available A podridão-mole causada por Pectobacterium carotovorum subsp. carotovorum (Pcc pode ocorrer em plantios de couve-chinesa (Brassica pekinensis com até 67% de incidência. O trabalho visou avaliar a sensibilidade in vitro de Pcc a bactericidas, o efeito de Mycoshield® nas dosagens de 3,0 e 1,5 g L-1, e de leveduras a 10(8 cel/mL no controle da doença em casa de vegetação e em campo. As plantas foram pulverizadas com Mycoshield® (oxitetraciclina 20% e leveduras (Rh1 e Rh2 (Rhodotorula spp. e Sc1 (Saccharomyces cerevisae sete dias após o transplante, e inoculadas por picada com o isolado Pcc120, sete dias e 12 h após o tratamento, respectivamente. Em todos os experimentos foram avaliados os componentes epidemiológicos da doença. In vitro, 40 isolados de Pcc testados apresentaram resistência ao sulfato de cobre e sensibilidade a oxitetraciclina, estreptomicina, oxitetraciclina+estreptomicina e oxitetraciclina+sulfato de cobre, todos na concentração de 0,2 g L-1. Seis isolados de Pcc foram mais inibidos por Mycoshield® do que por Agri-Micina® (oxitetraciclina 1,5% + estreptomicina 15% ambos a 3,0 g L-1, não sendo inibidos por Kasumin® (casugamicina 2% (2,0 mL L-1. Em casa de vegetação, Mycoshield® (3,0 g L-1 reduziu a severidade e o índice de doença em até 47,4 e 19%; já a levedura Sc1 reduziu a severidade da doença e a área abaixo da curva de progresso da doença (AACPD em até 27,6 e 39,3%, respectivamente, enquanto Rh1 reduziu a AACPD em até 33,5%. Em campo, Mycoshield® reduziu o índice de doença (14,4% a severidade (15,5% e a AACPD (28,9%; enquanto que Rh1 reduziu o índice de doença (8,8% e Sc1 reduziu a AACPD (15,7%. Conclui-se que o Mycoshield® e as leveduras apresentaram baixa eficiência para controle da podridão-mole da couve-chinesa em campo.The soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc may occur in Chinese cabbage (Brassica pekinensis plantations presenting till 67% of incidence

  16. Ab urbe condita. Desde a fundaçao da cidade de Bracara Augusta

    Directory of Open Access Journals (Sweden)

    Rui Morais

    2005-01-01

    Full Text Available Neste estudo aborda-se a problemática da fundaçao da antiga cidade de Bracara Augusta, levando em consideraçao o registro arqueológico e sua problemática e significado. A parte da fundaçao ex novo da cidade ou da preexistência de populaçoes autóctones no local, apresentam-se fortes vestígios indicadores da importância do culto imperial e dinástico que pensamos fortemente associados ao acto simbólico da sua fundaçao ou "refundaçao" segundo uma cerimónia à da "Roma Quadrada" augústea.

  17. Decisões judiciais sobre acesso aos medicamentos em Pernambuco

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    Artur Stamford

    2012-10-01

    Full Text Available OBJETIVO: Analisar as decisões do sistema jurídico sobre acesso da população a medicamentos no Sistema Único de Saúde por via judicial quanto a critérios de decisão e eventuais pressões políticas e econômicas. MÉTODOS: Estudo documental descritivo e retrospectivo de abordagem qualitativa e quantitativa. A coleta de dados foi realizada na Superintendência Estadual de Assistência Farmacêutica de Pernambuco, utilizando-se como fontes de dados 105 ações judiciais e relatórios administrativos de janeiro a junho de 2009. Verificaram-se os medicamentos com pedido ou carta patente no banco de dados do Instituto Nacional de Propriedade Industrial para identificar a frequência das patentes nas ações judiciais. Os dados obtidos foram classificados conforme o Sistema Anatômico Terapêutico Químico. Para análise das decisões judiciais, foi utilizada a teoria dos sistemas sociais autopoiéticos. RESULTADOS: As ações judiciais somaram 134 medicamentos com valor estimado de R$ 4,5 milhões para atender aos tratamentos solicitados; 70,9% dos medicamentos estavam com carta patente ou pedido e se concentraram em três classes terapêuticas: antineoplásicos e moduladores imunológicos, aparelho digestório e metabolismo, órgãos dos sentidos. Foram identificadas seis ideias centrais com os critérios de decisão dos magistrados (Constituição Federal e prescrição médica e as pressões entre os sistemas do direito, da economia e da política quanto ao acesso a medicamentos. CONCLUSÕES: A análise das decisões judiciais fundamentadas na teoria dos sistemas sociais autopoiéticos permitiu identificar as estimulações mútuas (dependências entre o sistema do direito e demais sistemas sociais em relação à questão do acesso dos cidadãos aos medicamentos. Essas dependências foram representadas pela Constituição Federal e pela propriedade intelectual. A Constituição Federal e a prescrição médica foram identificadas como

  18. Obesidade da genética ao ambiente

    Directory of Open Access Journals (Sweden)

    Odete Amaral

    2016-02-01

    Full Text Available A obesidade é reconhecida pela Organização Mundial de Saúde (OMS como um importante problema de saúde pública, afectando crianças, adolescentes e adultos (WHO, 1998. Os dados do International Obesity Task Force (2000 mostraram que, nos últimos anos, a prevalência de obesidade tem aumentado significativamente em várias regiões do mundo, sendo responsável, em grande parte, pelo aumento da mortalidade e morbilidade com implicações significativas no indivíduo, na família e na comunidade (Dietz et al, 1999; Cole et al, 2000. O impacto no indivíduo pode ser quantificado pela influência na qualidade de vida, no recurso aos serviços de saúde, no absentismo escolar, na limitação nas actividades de vida diária, nomeadamente na prática de desporto e alterações nas relações interpessoais, marginalização, depressão e isolamento. Ao nível da família, as repercussões podem traduzir-se no absentismo profissional, nas alterações dos estilos de vida e na necessidade de acompanhamento dos familiares.  O aumento da prevalência de obesidade e consequente aumento do risco de desenvolvimento de doenças crónicas na idade adulta, que lhe está associado, deve ser rigorosamente monitorizado (Cole et al, 2000; WHO, 1995. Essa monitorização DEVE permitir avaliar a evolução do problema, proceder a comparações entre várias regiões e ou países e determinar a eficácia das intervenções introduzidas para minimizar o problema. Para que tal aconteça, torna-se necessário uniformizar os conceitos de excesso de peso e obesidade para que possam ser amplamente utilizados.

  19. Robo-AO KP: A new era in robotic adaptive optics

    Science.gov (United States)

    Riddle, Reed L.; Baranec, Christoph; Law, Nicholas M.; Kulkarni, Shrinivas R.; Duev, Dmitry; Ziegler, Carl; Jensen-Clem, Rebecca M.; Atkinson, Dani Eleanor; Tanner, Angelle M.; Zhang, Celia; Ray, Amy

    2016-01-01

    Robo-AO is the first and only fully automated adaptive optics laser guide star AO instrument. It was developed as an instrument for 1-3m robotic telescopes, in order to take advantage of their availability to pursue large survey programs and target of opportunity observations that aren't possible with other AO systems. Robo-AO is currently the most efficient AO system in existence, and it can achieve an observation rate of 20+ science targets per hour. In more than three years of operations at Palomar Observatory, it has been quite successful, producing technology that is being adapted by other AO systems and robotic telescope projects, as well as several high impact scientific publications. Now, Robo-AO has been selected to take over operation of the Kitt Peak National Observatory 2.1m telescope. This will give Robo-AO KP the opportunity to pursue multiple science programs consisting of several thousand targets each during the three years it will be on the telescope. One-sixth of the observing time will be allocated to the US community through the NOAO TAC process. This presentation will discuss the process adapting Robo-AO to the KPNO 2.1m telescope, the plans for integration and initial operations, and the science operations and programs to be pursued.

  20. A avaliçao da concretizaçao do processo de Bolonha numa instituiçao de ensino superior portuguesa

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    Pedro Rocha Dos Reis

    2009-01-01

    Full Text Available A questão central do Processo de Bolonha consiste na mudança de paradigma de ensino, implicando a transição de um modelo passivo, fortemente centrado na aquisição de conhecimentos, para um modelo baseado no desenvolvimento de competências, onde a componente experimental e de projecto desempenham um papel importante. Concluídos dois anos de funcionamento dos seus cursos de 1º Ciclo, a Escola Superior de Educação de Santarém (Portugal procedeu a uma avaliação da implementação do Processo de Bolonha ao nível dos cursos. Este artigo apresenta os resultados dessa reflexão alargada. A partir da análise de dados recolhidos junto dos coordenadores (através de entrevista e dos professores e alunos dos cursos (através de questionário, conclui-se que o balanço da implementação do Processo de Bolonha é francamente positivo. Com efeito, permitiu, por um lado, uma maior reflexão sobre os perfis profissionais e centrar o processo formativo nas experiências educativas e nas aprendizagens, nomeadamente as adquiridas no âmbito dos programas de mobilidade, por outro lado, permitiu também uma maior articulação entre a actividade docente, de tutoria e a componente prática. Apesar de tudo, subsistem questões por aprofundar emelhorar, as quais irão sendo trabalhadas em função das avaliações que forem sendo feitas.The main issue of the Bologna’s Process consists in a change of the teaching paradigm, implying the transition of a passive model, strongly focused in the knowledge acquisition, for a model based in the skills development, where the experimental component and the project play an important role. Completed two years of operation of their courses of the FirstCicle, the Higher School of Education of Santarem (Portugal carriedon with the evaluation of the implementation of the Bologna’s Process at the courses’level. This article presents the results of this extended reflection.From the analysis of data collected from

  1. Subsunção do trabalho imaterial ao capital

    Directory of Open Access Journals (Sweden)

    Eleutério F. S. Prado

    Full Text Available O presente artigo examina a transformação do modo de produção capitalista que acompanha a expansão do trabalho imaterial como fonte de geração de riqueza efetiva. Essa avaliação é feita no interior da teoria do valor-trabalho e da crítica da economia política. Para tanto, interroga, primeiro, o verdadeiro impacto da informatização na natureza capitalista dos processos produtivos atualmente em andamento, principalmente nos países centrais. Compara, depois, as principais características da indústria taylorista com as características marcantes da indústria pós-taylorista. Ao final, conclui que uma transformação importante do sistema capitalista está em curso: a grande indústria, tal como foi caracterizada por Marx em O Capital, não predomina mais nos países do centro do sistema, pois o que aí prevalece agora é a pós-grande indústria.

  2. Do Antissocialismo ao Anticapitalismo: um estudo sobre a Rerum Novarum

    Directory of Open Access Journals (Sweden)

    Claudinei Magno Magre Mendes

    2016-05-01

    Full Text Available O objetivo deste artigo é examinar o processo de constituição do anticapitalismo por meio da análise da Rerum Novarum, a primeira encíclica social. Comparando esta com as encíclicas anteriores do século XIX, pudemos estabelecer o que ela trouxe de novidade no que diz respeito ao combate que a Igreja travava contra o socialismo. Com essa comparação, foi possível caracterizar a postura anticapitalista, confrontando a nossa maneira de concebê-la com as demais. Para o exame da Rerum Novarum foi igualmente importante analisar alguns estudos referentes a ela, tanto os que apareceram logo após a sua publicação como os que foram realizados em décadas mais recentes. Como conclusão, definimos o anticapitalismo como uma atitude que somente poderia ter surgido em condições sociais e políticas bastante particulares e que apenas apareceram em fins do século XIX.

  3. FIREWALL E SEGURANÇA DE SISTEMAS APLICADO AO LINUX

    Directory of Open Access Journals (Sweden)

    Rodrigo Ribeiro

    2017-04-01

    Full Text Available Tendo em vista a evolução da internet no mundo, torna-se necessário investir na segurança da informação, alguns importantes conceitos referentes as redes de computadores e sua evolução direcionam para o surgimento de novas vulnerabilidades. O objetivo principal deste trabalho é comprovar que, por meio da utilização de software livre como o Linux e suas ferramentas, é possível criar um cenário seguro contra alguns ataques, por meio de testes em ambientes controlados utilizando-se de arquiteturas testadas em tempo real e verificando qual o potencial de uso entre uma pesquisa autoral sobre o assunto, a partir dessa ideia, foi possível reconhecer a grande utilização dos mecanismos de segurança, validando a eficiência de tais ferramentas estudadas na mitigação de ataques a redes de computares. Os sistemas de defesa da plataforma Linux são extremamente eficientes e atende ao objetivo de prevenir uma rede de acesso indevido.

  4. [Variability of Cytochrome b Gene and Adjacent Section of Gene tRNA-Thr of Mitochondrial DNA in the Northern Mole Vole Ellobius talpinus (Mammalia, Rodentia)].

    Science.gov (United States)

    Bogdanov, A S; Lebedev, V S; Zykov, A E; Bakloushinskaya, I Yu

    2015-12-01

    The Northern mole vole E. talpinus, despite its wide distribution, is characterized by a stable karyotype (2n = NF = 54) and slight morphological polymorphism. We made a preliminary analysis of a mitochondrial DNA fragment to clarify the level of genetic variation and differentiation of E. talpinus. the complete cytochrome b gene (cyt b, 1143 bp) and a short part of its flanking gene tRNA-Thr (27 bp) were sequenced. We studied 16 specimens from eight localities, including Crimea, the Volga region, the Trans-Volga region, the Southern Urals, Western Siberia, and Eastern Turkmenistan. Mitotypes of E. talpinus were distributed on a ML dendrogram as four distinct clusters: the first (I) contains specimens from the Crimea, the second (II) combines individuals from the Volgograd region and the left bank of the Don River, the third (III) includes those from the Trans-Volga region, Southern Urals, the left bank of the Irtysh River, and Eastern Turkmenistan; the fourth (IV) are those from the right bank of the Irtysh River. These clusters were relatively distant from each other: the mean genetic distances (D) between them are 0.021-0.051. The Eastern mole vole E. tancrei differed from E. talpinus population groups 1.5-2 times more (D = 0.077-0.084) than the latter did among themselves. Such variations indirectly proved the unity of E. talpinus, despite its high intraspecific differentiation for the studied fragment of mitochondrial DNA. This differentiation apparently occurred because of the long isolation of E. talpinus population groups, which was due to geographic barriers, in particular, the large rivers that completely separate the species range meridionally (the Volga River, the Irtysh River). Sociality and underground lifestyle could accelerate the fixation of mutations in disjunct populations. The composition and distribution of intraspecific groups of E. talpinus, which were identified in analysis of the mitochondrial DNA fragment, do not coincide with the

  5. ROTEIRO DE VIAGEM AO PAÍS DE LACAN

    Directory of Open Access Journals (Sweden)

    Laéria Fontenele

    2015-12-01

    Full Text Available Grande leitor e crítico da cultura de seu tempo e um dos nomes de maior destaque da psicanálise contemporânea, Slavoj Žižek desfaz a fábula segundo a qual ler Lacan é um trabalho árduo e difícil que seria apenas privilégio dos especialistas em psicanálise e, com isso, brinda o seu leitor com um envolvente passeio pelo país de Lacan. De origem eslovena, e quase eleito presidente de seu país, Slavoj Žižek é autor de diversos livros - dentre os quais os instigantes ensaios “Hegel, o mais sublime dos histéricos” e “Eles não sabem o que dizem” -, a maioria publicada em por volta de trinta idiomas. É ele reconhecido como sendo um profundo conhecedor de Hegel e Karl Marx; apaixonado leitor de Sigmund Freud e de Jacques Lacan; crítico contundente das ideologias totalitárias, espírito cultivado por sua filia às múltiplas artes – sendo, inclusive, especialista na cinematografia de Alfred Hitchcock. Os traços de sua erudição e sensibilidade para com as artes, se refletem e particularizam, também, o seu estilo de escrever, o qual se encontra muito bem representado no modo como urdiu esse seu novo livro. “Como ler Lacan” nos ensina a adotar um método especial para orientar em uma tal empreitada. Žižek advoga que, para lermos Lacan, devemos aplicar o mesmo método empregado pela psicanálise para ler e interpretar o texto que resulta do trabalho inconsciente. Mas há uma condição para esse emprego: abandonar o vício de querer entender, pois, assim como o inconsciente, Lacan não deve ser compreendido e sim lido, pois, conforme ele mesmo já nos advertira, o pior destino a que podemos ser conduzidos é o destino que nos leva à compreensão. Só assim será fácil ler Lacan, mais do que, amiúde, se imagina. Muitas introduções ao seu ensino foram publicadas, algumas delas marcadas por rigor conceitual, outras resultaram em versões grosseiras de sua letra. Sem desmerecer as boas introduções a Lacan

  6. INTRODUÇAO AOS MODELOS ODE PARTICIPAÇÃO DE MERCADO EM SERVIÇOS DE TRANSPORTES

    Directory of Open Access Journals (Sweden)

    Antonio Galvão Novaes

    2010-04-01

    Full Text Available

    Análises de participação de mercado ("market share" são comuns em estudos na área de "marketing", envolvendo produtos e respectivas marcas, de um lado, o perfil da demanda de outro, e o posicionamento das empresas produtoras que competem nesse mercado, num terceiro bloco. No setor de transportes a literatura registra aplicações em ligações aéreas e em serviços intermunicipais de ônibus. Os modelos de participação de mercado se aplicam normalmente a situações em que os operadores oferecem serviços de transportes similares num determinado mercado, com a competição sendo feita não com base em atributos modais intrínsecos, mas sim apoiada em características diferenciadoras intra-modais mais tênues, tais como conforto, atendimento, promoções tarifárias, "marketing" e propaganda, etc. Neste artigo é feita uma introdução aos modelos de "market share" em transportes, com a apresentação de um exemplo de aplicação ao transporte intermunicipal de passageiros em ônibus, sendo discutidos também aspectos ligados a calibração e tais modelos.

    ABSTRACT

    Market share analysis is common in marketing studies involving products and respective brands, on one hand, the demand profile on the other hand, and the competiting industries on a third set. In the transport sector the literature registers a number of applications to air transport problems and to intercity bus services. Market share models are normally applied to situations in which operators offer, in a specific market, transport services that are similar in nature, with competition based not on intrinsic modal attributes, but rather on more tenous intra-mode differentiating characteristics, such as comfort, attendance, tariff promotions, marketing and advertising efforts, etc (sub-modal configurations. It is presented in this paper an introduction to market share modelling in transport, with an example concerning an intercity bus service in

  7. A low-fluorine solution with a 2:1 F/Ba mole ratio for the fabrication of YBCO films

    DEFF Research Database (Denmark)

    Wu, Wei; Feng, Feng; Yue, Zhao

    2014-01-01

    must be at least 2 for full conversion of the Ba-precursor to BaF2 to avoid the formation of BaCO3, which is detrimental to the superconducting performance of YBCO films. In this study, a solution with a 2:1 F/Ba mole ratio was developed, and the fluorine content of this solution was approximately only...... 10.3% of that used in the conventional TFA-MOD method. Attenuated total reflectance-Fourier transform-infrared spectra (ATR-FT-IR) revealed that BaCO3 was remarkably suppressed in the as-pyrolyzed film—and eliminated at 700 °C. Thus, YBCO films with a critical current density (Jc) of over 5 MA cm−2...... (77 K, 0 T, 200 nm thickness) could be obtained on lanthanum aluminate single-crystal substrates. In situ FT-IR spectra showed that no obvious fluorinated gaseous by-products were detected in the pyrolysis step, which indicated that all F atoms might remain in the film as fluorides. X-ray diffraction...

  8. Biomonitoring of the genotoxic effects and oxidative potentials of commercial edible dung beetles (Onitis sp.), grasshopper (Caelifera sp.) and mole crickets (Gryllotalpa sp.) in vitro.

    Science.gov (United States)

    Koc, Kubra; Incekara, Umit; Turkez, Hasan

    2014-09-01

    In this investigation, the genotoxic and oxidative effects of water soluble extracts of dung beetles, flying grasshopper and mole crickets have been assessed on cultured human blood cells. The extracts were added to the culture tubes at 12 different concentrations (0-2000 ppm). Micronucleus test was used to monitor the DNA and the chromosomal damage produced by aqueous extracts in vitro. In addition, to assess the oxidative effects, total antioxidant capacity (TAC) and total oxidant status (TOS) levels were also measured. Our results indicated that these extracts did not show genotoxic effects at the tested concentrations. However, the extracts caused dose-dependent alterations in both TAC and TOS levels. Based on the findings, it was concluded that the studied insects can be consumed safely, but it is necessary to consider the cellular damages which are likely to appear depending on oxidative stress at higher concentrations. It has also been suggested that this in vitro approach for oxidative and genotoxicity assessments may be useful to evaluate the potential health risks of edible insects. © The Author(s) 2014.

  9. Amyloid beta and the longest-lived rodent: the naked mole-rat as a model for natural protection from Alzheimer's disease.

    Science.gov (United States)

    Edrey, Yael H; Medina, David X; Gaczynska, Maria; Osmulski, Pawel A; Oddo, Salvatore; Caccamo, Antonella; Buffenstein, Rochelle

    2013-10-01

    Amyloid beta (Aβ) is implicated in Alzheimer's disease (AD) as an integral component of both neural toxicity and plaque formation. Brains of the longest-lived rodents, naked mole-rats (NMRs) approximately 32 years of age, had levels of Aβ similar to those of the 3xTg-AD mouse model of AD. Interestingly, there was no evidence of extracellular plaques, nor was there an age-related increase in Aβ levels in the individuals examined (2-20+ years). The NMR Aβ peptide showed greater homology to the human sequence than to the mouse sequence, differing by only 1 amino acid from the former. This subtle difference led to interspecies differences in aggregation propensity but not neurotoxicity; NMR Aβ was less prone to aggregation than human Aβ. Nevertheless, both NMR and human Aβ were equally toxic to mouse hippocampal neurons, suggesting that Aβ neurotoxicity and aggregation properties were not coupled. Understanding how NMRs acquire and tolerate high levels of Aβ with no plaque formation could provide useful insights into AD, and may elucidate protective mechanisms that delay AD progression. Copyright © 2013 Elsevier Inc. All rights reserved.

  10. Mola hidatiforme en un embarazo ectópico. Presentación de un caso Hydatid mole in an ectopic pregnancy

    Directory of Open Access Journals (Sweden)

    Vladimir Díaz Noda

    2005-09-01

    Full Text Available La Mola Hidatiforme se caracteriza por la presencia de anormalidades de las vellosidades coriónicas que consisten en grados variables de proliferación trofoblásticas y de edema del estroma de las vellosidades.Su incidencia varia de manera importante en las diversas regiones del mundo, no ocurriendo así con el embarazo ectópico que ha tenido un aumento significativo en todo el planeta. Pero la asociación de estas dos entidades es muy rara. Presentamos un caso de 35 años de edad donde observamos esta rara asociación, en el que se realizó salpingectomia derecha con evolución favorable.The Hydatiform mole is characterized by the presence of abnormalities of chorionic vellus which consist of variables degrees of trophoblastic proliferation and edema of the vellus stroma. Its incidence varies very importantly in the varuopus regions all over the world, which is not the same for the ectopic pregnancy that has increased significantly around the globe. The association between these two entities is uncommon. We presented a case with a 35 year - old person and observed this uncommon association, and performed a right salpingectomy with favorable progress.

  11. Efeitos do ruído de tráfego na saúde: para além das "moléstias"

    Directory of Open Access Journals (Sweden)

    Julio Díaz Jiménez

    2015-12-01

    Full Text Available Os transtornos auditivos têm sido tradicionalmente associados ao ruído laboral das pessoas expostas. Recentemente, o ruído produzido nas proximidades de aeroportos foi relacionado com diferentes patologias, sobretudo as cardiovasculares. Nos últimos anos, o ruído ambiental das grandes cidades, principalmente devido ao tráfego, foi relacionado com diversas patologias, tanto de natureza cardiovascular, como respiratória e inclusivamente com diabetes. Neste trabalho, para além se fazer uma breve descrição dos estudos que relacionam o ruído ambiental com a morbilidade e os diferentes mecanismos biológicos implicados, possíveis de explicar nessas associações; apresentam-se os resultados de um conjunto de investigações que, mediante a Análise de Séries Temporais, relacionam o ruído de tráfego na cidade de Madrid com a mortalidade diária por causas circulatórias, respiratórias e diabetes na faixa etária de maiores de 65 anos. Assim como a mortalidade anual atribuída ao ruído de tráfego em Madrid e a sua comparação com a atribuição às PM2,5.

  12. As hérnias medem-se aos palmos?

    Directory of Open Access Journals (Sweden)

    Cátia Pereira

    2016-06-01

    Full Text Available Apresenta-se o caso de uma doente do sexo feminino com 74 anos, autónoma, com antecedentes patológicos de obesidade, diabetes mellitus tipo 2, hipertensão arterial e doença osteoarticular degenerativa, que recorreu à urgência por infeção respiratória inferior. Negava sintomas gastrointestinais ou antecedentes cirúrgicos. Ao exame objetivo, destacava-se a presença de uma hérnia umbilical volumosa não dolorosa à palpação, mas que condicionava a sua mobilidade, sendo impossível a redução do conteúdo herniário. Após insistência na anamnese, a doente referiu o seu aparecimento na infância, na altura com recusa em efetuar a sua correção cirúrgica, tendo sido perdido o seu seguimento posteriormente. A radiografia e a tomografia computorizada abdominais mostraram um colo herniário de seis centímetros de diâmetro e herniação de praticamente todo o intestino delgado e parte do cólon, sem evidência de encarceramento (Figuras 1 e 2. Apesar de décadas de evolução condicionarem um progressivo e forte impacto nas atividades da vida diária, a doente não procurou os cuidados de saúde por esse motivo, mantendo a sua decisão e sendo encaminhada para a consulta externa. As complicações herniárias abdominais incluem a obstrução intestinal e a temida emergência cirúrgica da hérnia encarcerada, não se desprezando a morbilidade inerente, bem evidente neste caso.1-2

  13. O sindicalismo mineiro frente aos desafios dos anos 90

    Directory of Open Access Journals (Sweden)

    Antônio Carvalho Neto

    2000-12-01

    Full Text Available O que se analisa neste artigo é como os sindicatos dos bancários, metalúrgicos e trabalhadores em telecomunicações têm negociado o (desemprego, a demanda por qualificação e a remuneração variável. Foram tabuladas/analisadas 3.569 cláusulas de acordos coletivos, e realizadas 26 entrevistas com dirigentes sindicais e empresariais em três Estados (RJ, SP e MG. Os resultados indicam que as mudanças organizacionais foram incomparavelmente mais negociadas no setor metalúrgico do ABC. A flexibilização da jornada foi trocada pela redução (da jornada sem redução de salário nos setores metalúrgicos paulistas, mas não em Minas Gerais. No setor bancário, houve poucos resultados nas negociações sobre jornada. As negociações em torno da remuneração variável vinculada às metas das empresas aconteceram nos setores metalúrgicos, e não nos setores de serviços. As entrevistas apontam para um sindicalismo mais propositivo, no sentido de uma intervenção negociada, predisposição que não é correspondida pelos empresários, grosso modo. O Estado, que poderia utilizar seu poder regulador para induzir os empresários a negociarem nessa direção não tem produzido resultados satisfatórios. Conclui-se que não há maturidade empresarial no Brasil que permita ao Estado ausentar-se das relações de trabalho.

  14. Tecnologias e letramento digital: navegando rumo aos desafios

    Directory of Open Access Journals (Sweden)

    Ivanda Martins Silva

    2011-12-01

    Full Text Available Computadores, celulares, redes sociais, ferramentas de pesquisa e interação on-line são recursos utilizados pelos alunos no mundo dinâmico da cibercultura. As tecnologias da informação e comunicação estão exigindo leitores e produtores de textos ainda mais participativos. Aos textos impressos, somam-se os hipertextos e os suportes eletrônicos, que demandam diferentes estratégias de leitura e escrita. Será que a escola consegue criar espaços para as práticas de letramento digital dos alunos? Os professores precisam aprender a gerenciar as tecnologias da informação e comunicação. Ensinar com e para o uso crítico das tecnologias é função primordial da escola no processo de inclusão digital. Nesse sentido, não basta apenas usar aleatoriamente as tecnologias, sem planejamentos ou objetivos claros, mas sim transformar as relações dos sujeitos com os suportes tecnológicos. O presente trabalho discute a inserção das tecnologias na escola e apresenta contribuições para repensar o planejamento didático, considerando a diversidade de recursos tecnológicos que os professores podem utilizar na organização de situações de aprendizagem que levem o aluno a aprimorar as práticas de letramento digital.

  15. The nature of ethnic identity among the people of Curaçao

    NARCIS (Netherlands)

    Dijs, N.M. van der

    2011-01-01

    This qualitative research explores the nature of ethnic identity among the people of Curaçao. The questions that are of fundamental interest to this study are: how do the people of Curaçao perceive themselves ethnically, how do they experience their sense of belonging to an ethnic group and what

  16. Optimal control strategy to reduce the temporal wavefront error in AO systems

    NARCIS (Netherlands)

    Doelman, N.J.; Hinnen, K.J.G.; Stoffelen, F.J.G.; Verhaegen, M.H.

    2004-01-01

    An Adaptive Optics (AO) system for astronomy is analysed from a control point of view. The focus is put on the temporal error. The AO controller is identified as a feedback regulator system, operating in closed-loop with the aim of rejecting wavefront disturbances. Limitations on the performance of

  17. Freshwater Bryozoa (Phylactolaemata) from Curaçao, Aruba and Bonaire

    NARCIS (Netherlands)

    Lacourt, A.W.

    1955-01-01

    The Phylactolaemata-fauna of Curaçao, Aruba and Bonaire, as it appears from the samples collected by dr P. Wagenaar Hummelinck, is connected with the fauna of the South-American mainland. The material belongs to three species only: Hyalinella agilis — Curaçao, Aruba, Bonaire — in temporary or

  18. Propriedades tecnológicas do lenho de louro-preto

    Directory of Open Access Journals (Sweden)

    Jordão Cabral Moulin

    2017-01-01

    Full Text Available Os estudos de caracterização da madeira permitem a sua otimização nos diversos segmentos madeireiros, principalmente o beneficiamento da madeira. O presente trabalho teve por objetivo caracterizar as propriedades da madeira de Cordia glabrata (louro-preto, visando aumentar o conhecimento a respeito do potencial de espécies madeireiras menos tradicionais. Foi realizada a caracterização macroscópica e microscópica, análises químicas, físicas e avaliadas algumas propriedades mecânicas da madeira de C. glabrata. A madeira apresenta altos teores de lignina total, fibras longas e com espessura de parede média. Foi classificada como madeira de alta densidade e média estabilidade dimensional. A espécie também possui valores médios de resistência à compressão paralela e módulo de ruptura, altos valores de tensão de cisalhamento e valores considerados baixos pelos teste de fendilhamento. C. glabrata possui características adequadas para ser utilizada em diversas finalidades no setor madeireiro brasileiro, especialmente para o mercado de lâminas decorativas, pisos, em usos que requerem boa durabilidade natural e certa resistência mecânica.

  19. ANATOMIA DO LENHO DE MURTA – Blepharocalyx salicifolius (H.B.K. Berg

    Directory of Open Access Journals (Sweden)

    José Newton Cardoso Marchiori

    2005-08-01

    Full Text Available O presente trabalho teve por objetivo a descrição da estrutura anatômica da madeira de murta – Blepharocalyx salicifolius (H.B.K. Berg (Myrtaceae. Foram coletados seis exemplares da espécie, sendo três na região da Serra do Sudeste e três nos Campos de Cima da Serra, RS. Para confecção de lâminas histológicas, seguiu-se a técnica padrão, utilizada no Laboratório de Anatomia da Madeira da Universidade Federal do Paraná. A maceração da madeira foi realizada pelo método de Jeffrey. A estrutura anatômica da madeira coincide, em linhas gerais, com o padrão típico da família, reunindo um grande número de poros estreitos e solitários, com placas de perfuração simples, além de parênquima apotraqueal difuso, raios heterogêneos e fibras com pontoações areoladas. A presença de monocristais rombóides, bem como a ausência de traqueídeos vasicêntricos permite distinguir a espécie de outras Mirtáceas sul-rio-grandenses anteriormente descritas.

  20. DESCRIÇÃO ANATÔMICA DO LENHO DE Melaleuca leucadendron (L. L

    Directory of Open Access Journals (Sweden)

    Linéia Roberta Zen

    2016-03-01

    Full Text Available This work aimed the anatomical description of Melaleuca leucadendron wooden. The study was conducted from an individual tree collecting three discs for confection of histological slides and macerated. Was used to mashing nitricacetic method and for histology was used simple staining with 1% safranin. The wood anatomy agrees in general aspects to the typical pattern of Myrtaceae Family: flocking diffuse porosity, solitary pores, simple perforation plates, besides paratracheal axial parenchyma linear aliforme, very numerous rays and heterocellular, pits-ray vascular parenchyma-vascular and fibrous tissue composed of prominent fibrotraqueoids, very short fibers, distinct growth rings of walls marked by the end of the fibers flattened latewood. Qualitatively and quantitatively the wood did not reveal expressive characteristic, thus can be characterized by presenting a relatively simple anatomical structure, agreeing in general lines to Myrtaceae family.

  1. Research on AO/FO batch management technology in aircraft production

    Directory of Open Access Journals (Sweden)

    Yin Haijun

    2018-01-01

    Full Text Available Based on the analysis of the characteristics and significance of AO/FO in the process of aircraft production, this paper analyzes the format rules of AO/FO batch management from the perspective of technology realization, and details the AO/FO The change of the query and the change status tracking, introduces the AO/FO single-stand status display in the batch management, increases the structure definition of the attribute table in the batch management, and designs the relevant algorithm to store and calculate the batch information. Finally, based on the above theory support AO/FO batch management system successfully used in the production of a machine.

  2. Common Moles, Atypical Moles (Dysplastic Nevi), and Risk of Melanoma

    Science.gov (United States)

    ... Data Conducting Clinical Trials Statistical Tools and Data Terminology Resources NCI Data Catalog Cryo-EM NCI's Role ... freckles have an increased chance of melanoma. Certain medical conditions or medicines : Medical conditions or medicines (such ...

  3. Um sopro de Luz e Letra em meio ao caos

    Directory of Open Access Journals (Sweden)

    Marcelo Tomazi

    2009-06-01

    Full Text Available Enquanto compreende avanços científicos, o homem busca compreender a si mesmo. Partindo da citação de Carl Sagan, vemos que a Arte é um dos caminhos para a humanização, e os vínculos entre ciência (tecnologia e arte são cada vez mais estreitos. Um dos artistas que melhor trabalham dentro do nicho conhecido como “arte tecnológica” é Eduardo Kac. Sua trajetória inicial é apresentada aqui, dos “holopoemas” à “arte da telepresença”. Vemos o artista-teórico completo, profundo conhecedor da história da arte eletrônica. O livro Luz & letra: ensaios de arte, literatura e comunicação, sua obra de referência, é apresentado em essência, situando-se sua importância. É uma das poucas obras no Brasil a discutir, pela ótica de quem fez parte, eventos ligados ao advento da arte tecnológica nos anos 1980.In our quest to understand scientific advances, we seek to understand ourselves. Employing Carl Sagan's insight as a departure point, we see that art is one of the paths for humanization and that the links between science (technology and art are increasingly narrow. One of the artists who best works within the realm known as “art and technology” is Eduardo Kac. This essay explores Kac's early artworks, starting with “holopoems” and arriving at “telepresence art”, and especially his texts. We see a complete artist-theorist, with an unrivaled knowledge of electronic art and literature, in part because his theorizing emerges from having actively participated in the creation of the events he refers to. Of special interest is Kac's book Light & letter: essays in art, literature, and communication, which collects his writings of the 1980s. This is a fundamental work and mandatory reference for the study of the experimental art and literature of the period.

  4. Schedule control in Ling Ao nuclear power project

    International Nuclear Information System (INIS)

    Xie Ahai

    2007-01-01

    Ling Ao Nuclear Power Station (LANP) is first one built up by self-reliance in China with power capacity 990x2 MWe. The results of quality control, schedule control and cost control are satisfactory. The commercial operation days of Unit 1 and Unit 2 were 28th May 2002 and 8th Jan. 2003 respectively, which were 48 days and 66 days in advance of the project schedule. This paper presents the practices of self-reliance schedule control system in LANP. The paper includes 10 sections: schedule control system; targets of schedule control; schedule control at early stage of project; construction schedule; scheduling practice; Point curves; schedule control of design and procurement; a good practice of construction schedule control on site; commissioning and startup schedule; schedule control culture. Three figures are attached. The main contents of the self-reliance schedule control system are as follows: to draw up reasonable schedules and targets; to setup management mechanism and procedures; to organize powerful project management team; to establish close monitoring system; to provide timely progress reports and statistics information. Five kinds of schedule control targets are introduced, i.e. bar-chart schedule; milesones; Point curves; interface management; hydraulic test schedule of auxiliary piping loops; EMR/EMC/EESR issuance schedules. Six levels of bar-chart schedules were adopted in LANP, but the bar-chart schedules were not satisfactory for complicated erection condition on site, even using six levels of schedules. So a kind of Point curves was developed and their advantages are explained. Scheduling method of three elements: activity, duration, logic, which was adopted in LANP, is introduced. The duration of each piping activities in LANP level 2 project schedule was calculated based on the relevant working Point quantities. The analysis and adjustment of Point curves are illustrated, i.e. balance of monthly quantities; possible production in the peakload

  5. O direito fundamental ao conteúdo do próprio trabalho: uma reconstrução normativa do direito ao trabalho

    Directory of Open Access Journals (Sweden)

    Leonardo Vieira Wandelli

    2016-12-01

    Full Text Available Resumo: Pretende-se enfrentar a inefetividade do direito humano e fundamental ao trabalho a partir de um esforço de reconstrução normativa da sua fundamentação, apoiada em um duplo pilar teórico, nas teorias das necessidades e nas teorias do reconhecimento. Com isso, não somente se recupera a importância do vínculo entre trabalho e dignidade humana, como se explicitam aspectos essenciais da centralidade do trabalho para os sujeitos. A partir disso, o direito ao trabalho se desvela não apenas como necessidade radical, tendente à transformação da institucionalidade vigente, mas também como direito positivado em um amplo leque de normas internacionais e nacionais, que contempla diversas dimensões normativas atualmente exigíveis. Propõe-se que, no centro desse direito, está um direito fundamental ao conteúdo do próprio trabalho, que determina juridicamente as condições da atividade e da organização do trabalho. Recente estudo adotado pelo Alto Comissariado em Direitos Humanos da Organização das Nações Unidas (ONU sobre o direito ao trabalho reforça essa compreensão. Palavras-chave: Direitos humanos. Direitos fundamentais. Direito ao trabalho. Psicodinâmica do trabalho. Direito do trabalho.

  6. Tunable diode laser absorption spectroscopy-based tomography system for on-line monitoring of two-dimensional distributions of temperature and H{sub 2}O mole fraction

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Lijun, E-mail: lijunxu@buaa.edu.cn; Liu, Chang; Jing, Wenyang; Cao, Zhang [School of Instrument Science and Opto-Electronic Engineering, Beihang University, Beijing 100191 (China); Ministry of Education’s Key Laboratory of Precision Opto-Mechatronics Technology, Beijing 100191 (China); Xue, Xin; Lin, Yuzhen [School of Energy and Power Engineering, Beihang University, Beijing 100191 (China)

    2016-01-15

    To monitor two-dimensional (2D) distributions of temperature and H{sub 2}O mole fraction, an on-line tomography system based on tunable diode laser absorption spectroscopy (TDLAS) was developed. To the best of the authors’ knowledge, this is the first report on a multi-view TDLAS-based system for simultaneous tomographic visualization of temperature and H{sub 2}O mole fraction in real time. The system consists of two distributed feedback (DFB) laser diodes, a tomographic sensor, electronic circuits, and a computer. The central frequencies of the two DFB laser diodes are at 7444.36 cm{sup −1} (1343.3 nm) and 7185.6 cm{sup −1} (1391.67 nm), respectively. The tomographic sensor is used to generate fan-beam illumination from five views and to produce 60 ray measurements. The electronic circuits not only provide stable temperature and precise current controlling signals for the laser diodes but also can accurately sample the transmitted laser intensities and extract integrated absorbances in real time. Finally, the integrated absorbances are transferred to the computer, in which the 2D distributions of temperature and H{sub 2}O mole fraction are reconstructed by using a modified Landweber algorithm. In the experiments, the TDLAS-based tomography system was validated by using asymmetric premixed flames with fixed and time-varying equivalent ratios, respectively. The results demonstrate that the system is able to reconstruct the profiles of the 2D distributions of temperature and H{sub 2}O mole fraction of the flame and effectively capture the dynamics of the combustion process, which exhibits good potential for flame monitoring and on-line combustion diagnosis.

  7. High specific activity N-Acetyl-3{sup H}-{alpha}-Aspartyl- L-Glutamic at micro mole scale; Sintesis de N-Acetil-3{sup H}- {alpha} -Aspartil-Glutamico a escala de Micromoles

    Energy Technology Data Exchange (ETDEWEB)

    Suarez, C

    1984-07-01

    High specific activity N-Acetyl-3{sup H}- {alpha} -Aspartyl-I-Glutamic acid at micro mole scale in prepared acetylating L- {alpha} -Aspartyl-L-glutamic with 3{sup H}-acetic anhydride in re distilled toluene. The product le purified through cationic and anionic columns. The radiochemical purity as determined by thin-layer chromatography is greater then 99% at the time preparation. (Author) 5 refs.

  8. Ciências Humanas e Sociais: ciências moles? A propósito do trabalho científico nesta contemporaneidade

    Directory of Open Access Journals (Sweden)

    Maria Adélia Aparecida de Souza

    2011-07-01

    Full Text Available Este ensaio busca atender uma velha e interminável discussão sobre uma questão que longe de ser científica é, sobretudo, política, qual seja a classificação das ciências. A Geografia tem oscilado nessas classificações: ora é uma ciência humana, social, ora é uma ciência da natureza. Este estímulo é o motivo principal da realização deste ensaio que, inspirado em uma interessante reflexão de Abraham Moles, prêmio Nobel de Matemática, quando sugere que reflitamos com seriedade científica e acadêmica sobre esse debate. Esse autor propõe corajosamente que as ciências humanas sejam elas sim consideradas “ciências duras”. Aqui é defendida a tese de que, dadas as características e dinâmicas do mundo do presente, as classificações carecem de um processo de discussão condizente com a essência do mundo de hoje, que reverte por vezes o conhecimento pré-estabelecido. Sugere-se, por exemplo, nesta perspectiva, que o espaço geográfico, por ser uma instância social, tal como a economia, a política e a cultura, seja considerado uma categoria social de análise e não um palco de realização da vida, como tem sido considerado pela geografia descritiva, completamente ultrapassada pela velocidade do mundo e pelos sistemas técnicos disponíveis. Resumé SCIENCES HUMAINES ET SOCIALES: SCIENCES "MOLLES"? A PROPOS DU TRAVAIL SCIENTIFIQUE DANS LA CONTEMRAINETÉ Ce teste cherche à participer d´une interminable discussion sur question du classement des sciences. Catte discussion, loin d'être scientifique ou académique est uni discussion politique. Une source d'inspiration de cette reflexion a été I'oeuvre de Abraham Moles, Prix Nobel de Mathématique. Eant la Géographie une science que parfois est classée comme science de la nature, parfois comme science humaine et sociale. Catte discussion a inspirée aussi cet article. II attire I' ATTENTION SUR LA necessité, pour cette de prendre en consideration les

  9. Avaliação da qualidade de vida e vulnerabilidade ao estresse no contexto hospitalar

    Directory of Open Access Journals (Sweden)

    Fernanda Ottati

    2013-05-01

    Full Text Available O conceito de Qualidade de Vida é usado para medir as condições de vida de um ser humano, já a palavra vulnerabilidade refere-se à chance das pessoas estarem expostas ao adoecer. Objetivou-se analisar possíveis relações entre percepção de qualidade de vida e a vulnerabilidade ao estresse no trabalho em enfermeiros, técnicos e auxiliares de enfermagem que atuam na UTI e no setor de emergência de um hospital público. Utilizou-se o instrumento de qualidade de vida da Organização Mundial da Saúde, WHOQOL-Bref e a Escala de Vulnerabilidade ao Estresse no Trabalho. Encontrou-se, em relação ao setor de trabalho, diferença significativa apenas para Qualidade de vida total. Portanto pode-se afirmar que os profissionais que atuam na UTI possuem maior percepção de qualidade de vida quando comparados aos do SUS. Na correlação entre os dois instrumentos, o domínio meio ambiente do WHOQOL-Bref correlacionou-se negativa e significativamente com todos os fatores da EVENT, indicando que a percepção da pessoa em relação aos diversos aspectos do meio em que vive tem relação com a vulnerabilidade ao estresse.

  10. RECURSO AO LEGISLADOR: CONSIDERAÇÕES EM TORNO DO CONTROLE LEGISLATIVO AO PODER JUDICIÁRIO (UM EPITÁFIO À PEC Nº33)

    OpenAIRE

    Dettmam, Deborah; Matos, Nelson Juliano Cardoso

    2017-01-01

    Este artigo discute se o recurso ao povo ou ao legislador, como instrumento de resolução dos conflitos entre os poderes, viola a separação de poderes e a independência do poder judiciário ou se, antes disso, dá fiel execução à separação de poderes. Para alcançar esse objetivo, esse artigo investiga se existe relação entre o recurso ao legislador e os regimes autoritários; se a Constituição Federal de 1988 proíbe outro guardião constitucional, que não o Supremo Tribunal Federal, e quais as con...

  11. Multiple pregnancies with complete mole and coexisting normal fetus in North and South America: A retrospective multicenter cohort and literature review.

    Science.gov (United States)

    Lin, Lawrence H; Maestá, Izildinha; Braga, Antonio; Sun, Sue Y; Fushida, Koji; Francisco, Rossana P V; Elias, Kevin M; Horowitz, Neil; Goldstein, Donald P; Berkowitz, Ross S

    2017-04-01

    To determine the clinical characteristics of multiple gestation with complete mole and coexisting fetus (CHMCF) in North and South America. Retrospective non-concurrent cohorts compromised of CHMCF from New England Trophoblastic Disease Center (NETDC) (1966-2015) and four Brazilian Trophoblastic Disease Centers (BTDC) (1990-2015). From a total of 12,455 cases of gestational trophoblastic disease seen, 72 CHMCF were identified. Clinical characteristics were similar between BTDC (n=46) and NETDC (n=13) from 1990 to 2015, apart from a much higher frequency of potentially life-threatening conditions in Brazil (p=0.046). There were no significant changes in the clinical presentation or outcomes over the past 5 decades in NETDC (13 cases in 1966-1989 vs 13 cases in 1990-2015). Ten pregnancies were electively terminated and 35 cases resulted in viable live births (60% of 60 continued pregnancies). The overall rate of gestational trophoblastic neoplasia (GTN) was 46%; the cases which progressed to GTN presented with higher chorionic gonadotropin levels (p=0.026) and higher frequency of termination of pregnancy due to medical complications (p=0.006) when compared to those with spontaneous remission. The main regional difference in CHMCF presentation is related to a higher rate of potentially life-threatening conditions in South America. Sixty percent of the expectantly managed CHMCF delivered a viable infant, and the overall rate of GTN in this study was 46%. Elective termination of pregnancy did not influence the risk for GTN; however the need for termination due to complications and higher hCG levels were associated with development of GTN in CHMCF. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Caught in the act: Incipient speciation across a latitudinal gradient in a semifossorial mammal from Madagascar, the mole tenrec Oryzorictes hova (Tenrecidae).

    Science.gov (United States)

    Everson, Kathryn M; Hildebrandt, Kyndall B P; Goodman, Steven M; Olson, Link E

    2018-02-28

    Madagascar is one of the world's foremost biodiversity hotspots, yet a large portion of its flora and fauna remains undescribed and the driving forces of in situ diversification are not well understood. Recent studies have identified a widespread, latitudinally structured phylogeographic pattern in Madagascar's humid-forest mammals, amphibians, reptiles, and insects. Several factors may be driving this pattern, namely biogeographic barriers (i.e., rivers or valleys) or past episodes of forest contraction and expansion. In this study, we describe the phylogeographic structure of the small, semifossorial mammal Oryzorictes hova, one of Madagascar's two species of mole tenrec, found throughout Madagascar's eastern humid forest belt, from high-elevation montane forest to low-elevation forests, as well as disturbed habitat such as rice fields. Using one mitochondrial locus, four nuclear loci, and 31 craniomandibular measurements, we identified three distinct populations of O. hova associated with the northern, central, and southern regions of the island. We found little evidence of gene flow among these populations, so we treated each population as a potential species. We validated species limits using two Bayesian methods: BP&P, employing only DNA sequence data, and iBPP using both DNA and morphological data, and we assessed whether these methods are susceptible to producing false positive errors. Molecular and morphological data support the recognition of each of the three populations of O. hova as distinct species, but formal species descriptions will require additional data from type specimens. This study illustrates the importance of using integrative datasets, multiple methodological approaches, and extensive geographic sampling for species delimitation and adds evidence for a widespread phylogeographic pattern in Madagascar's humid forest taxa. Copyright © 2018 Elsevier Inc. All rights reserved.

  13. Influence of soft tissues on mandibular gray scale levels Influência dos tecidos moles sobre os níveis de cinza mandibulares

    Directory of Open Access Journals (Sweden)

    Paulo Henrique Couto Souza

    2004-03-01

    Full Text Available The purpose of this study was to analyze the gray levels, expressed in pixels, of the mandibular retromolar region, with regard to the influence of muscular and fat soft tissues near this region. Fifteen dry mandibles were X-rayed with the presence of soft tissue simulators. The radiographs were digitized and evaluated by Digora® software. A one cm thick layer of wax was used as a simulator of the muscular soft tissue. Animal fat samples of different thicknesses - 0.5, 1.0, 1.5 and 2.0 cm - were used as a simulator of the fat soft tissue. Results showed that the fat soft tissue simulator influenced the gray level values in pixels of the mandibular retromolar region when analyzed in different thicknesses using the Digora® digitized image software.O presente trabalho foi desenvolvido com o objetivo de analisar os níveis de cinza, expressos em "pixels", da região retromolar mandibular, considerando a influência dos tecidos moles muscular e, principalmente, adiposo adjacentes a essa região. Para o estudo, 15 mandíbulas secas foram tecnicamente radiografadas, sendo que as radiografias obtidas foram escaneadas e avaliadas pelo programa de imagens digitalizadas Digora®. Como simuladores dos tecidos muscular e adiposo, foram utilizadas cera utilidade na espessura de 1,0 cm e amostras de gordura animal em espessuras diferentes de 0,5; 1,0; 1,5 e 2,0 cm. Os resultados mostraram que o tecido adiposo foi capaz de influenciar a análise dos valores de níveis de cinza em "pixels" da região retromolar mandibular quando estudado em espessuras diferentes pelo programa de imagens digitalizadas Digora®.

  14. Socially regulated reproductive development: analysis of GnRH-1 and kisspeptin neuronal systems in cooperatively breeding naked mole-rats (Heterocephalus glaber).

    Science.gov (United States)

    Zhou, Shuzhi; Holmes, Melissa M; Forger, Nancy G; Goldman, Bruce D; Lovern, Matthew B; Caraty, Alain; Kalló, Imre; Faulkes, Christopher G; Coen, Clive W

    2013-09-01

    In naked mole-rat (NMR) colonies, breeding is monopolized by the queen and her consorts. Subordinates experience gonadal development if separated from the queen. To elucidate the neuroendocrine factors underlying reproductive suppression/development in NMRs, we quantified plasma gonadal steroids and GnRH-1- and kisspeptin-immunoreactive (ir) neurons in subordinate adults and in those allowed to develop into breeders, with or without subsequent gonadectomy. In males and females, respectively, plasma testosterone and progesterone are higher in breeders than in subordinates. No such distinction occurs for plasma estradiol; its presence after gonadectomy and its positive correlation with adrenal estradiol suggest an adrenal source. Numbers of GnRH-1-ir cell bodies do not differ between gonad-intact breeders and subordinates within or between the sexes. As in phylogenetically related guinea pigs, kisspeptin-ir processes pervade the internal and external zones of the median eminence. Their distribution is consistent with actions on GnRH-1 neurons at perikaryal and/or terminal levels. In previously investigated species, numbers of kisspeptin-ir cell bodies vary from substantial to negligible according to sex and/or reproductive state. NMRs are exceptional: irrespective of sex, reproductive state, or presence of gonads, substantial numbers of kisspeptin-ir cell bodies are detected in the rostral periventricular region of the third ventricle (RP3V) and in the anterior periventricular (PVa), arcuate, and dorsomedial hypothalamic nuclei. Nevertheless, the greater number in the RP3V/PVa of female breeders compared with female subordinates or male breeders suggests that emergence from a hypogonadotrophic state in females may involve kisspeptin-related mechanisms similar to those underlying puberty or seasonal breeding in other species. Copyright © 2013 Wiley Periodicals, Inc.

  15. Sox9 gene regulation and the loss of the XY/XX sex-determining mechanism in the mole vole Ellobius lutescens.

    Science.gov (United States)

    Bagheri-Fam, Stefan; Sreenivasan, Rajini; Bernard, Pascal; Knower, Kevin C; Sekido, Ryohei; Lovell-Badge, Robin; Just, Walter; Harley, Vincent R

    2012-01-01

    In most mammals, the Y chromosomal Sry gene initiates testis formation within the bipotential gonad, resulting in male development. SRY is a transcription factor and together with SF1 it directly up-regulates the expression of the pivotal sex-determining gene Sox9 via a 1.3-kb cis-regulatory element (TESCO) which contains an evolutionarily conserved region (ECR) of 180 bp. Remarkably, several rodent species appear to determine sex in the absence of Sry and a Y chromosome, including the mole voles Ellobius lutescens and Ellobius tancrei, whereas Ellobius fuscocapillus of the same genus retained Sry. The sex-determining mechanisms in the Sry-negative species remain elusive. We have cloned and sequenced 1.1 kb of E. lutescens TESCO which shares 75% sequence identity with mouse TESCO indicating that testicular Sox9 expression in E. lutescens might still be regulated via TESCO. We have also cloned and sequenced the ECRs of E. tancrei and E. fuscocapillus. While the three Ellobius ECRs are highly similar (94-97% sequence identity), they all display a 14-bp deletion (Δ14) removing a highly conserved SOX/TCF site. Introducing Δ14 into mouse TESCO increased both basal activity and SF1-mediated activation of TESCO in HEK293T cells. We propose a model whereby Δ14 may have triggered up-regulation of Sox9 in XX gonads leading to destabilization of the XY/XX sex-determining mechanism in Ellobius. E. lutescens/E. tancrei and E. fuscocapillus could have independently stabilized their sex determination mechanisms by Sry-independent and Sry-dependent approaches, respectively.

  16. Development of accurate dimethyl sulphide primary standard gas mixtures at low nanomole per mole levels in high-pressure aluminium cylinders for ambient measurements

    Science.gov (United States)

    Eon Kim, Mi; Kang, Ji Hwan; Doo Kim, Yong; Lee, Dong Soo; Lee, Sangil

    2018-04-01

    Dimethyl sulphide (DMS) plays an important role in atmospheric chemistry and climate change. Ambient DMS is monitored in a global network and reported at sub-nanomole per mole (nmol/mol) levels. Developing traceable, accurate DMS standards at ambient levels is essential for tracking the long-term trends and understanding the role of DMS in the atmosphere. Gravimetrically prepared gas standards in cylinders are widely used for calibrating instruments. Therefore, a stable primary standard gas mixture (PSM) is required for traceable ambient DMS measurement at remote sites. In this study, to evaluate adsorption loss on the internal surface of the gas cylinder, 6 nmol mol-1 DMS gas mixtures were prepared in three types of aluminium cylinders: a cylinder without a special coating on its internal surface (AL), an Aculife IV  +  III-treated cylinder (AC), and an Experis-treated cylinder (EX). There was little adsorption loss on the EX cylinder, whereas there was substantial adsorption loss on the other two cylinders. The EX cylinder was used to prepare 0.5, 2, 5, and 7 nmol mol-1 DMS PSMs with relative expanded uncertainties of less than 0.4%. The DMS PSMs were analytically verified and consistent within a relative expanded uncertainty of less than 1.2%. The long-term stability of the 7 nmol mol-1 DMS PSM was assessed by tracking the ratio of the DMS to the internal standard, benzene. The results showed that the DMS was stable for about seven months and it was projected to be stable for more than 60 months within a relative expanded uncertainty of 3%.

  17. And the beat goes on: maintained cardiovascular function during aging in the longest-lived rodent, the naked mole-rat.

    Science.gov (United States)

    Grimes, Kelly M; Reddy, Anilkumar K; Lindsey, Merry L; Buffenstein, Rochelle

    2014-08-01

    The naked mole-rat (NMR) is the longest-lived rodent known, with a maximum lifespan potential (MLSP) of >31 years. Despite such extreme longevity, these animals display attenuation of many age-associated diseases and functional changes until the last quartile of their MLSP. We questioned if such abilities would extend to cardiovascular function and structure in this species. To test this, we assessed cardiac functional reserve, ventricular morphology, and arterial stiffening in NMRs ranging from 2 to 24 years of age. Dobutamine echocardiography (3 μg/g ip) revealed no age-associated changes in left ventricular (LV) function either at baseline or with exercise-like stress. Baseline and dobutamine-induced LV pressure parameters also did not change. Thus the NMR, unlike other mammals, maintains cardiac reserve with age. NMRs showed no cardiac hypertrophy, evidenced by no increase in cardiomyocyte cross-sectional area or LV dimensions with age. Age-associated arterial stiffening does not occur since there are no changes in aortic blood pressures or pulse-wave velocity. Only LV interstitial collagen deposition increased 2.5-fold from young to old NMRs (P < 0.01). However, its effect on LV diastolic function is likely minor since NMRs experience attenuated age-related increases in diastolic dysfunction in comparison with other species. Overall, these findings conform to the negligible senescence phenotype, as NMRs largely stave off cardiovascular changes for at least 75% of their MLSP. This suggests that using a comparative strategy to find factors that change with age in other mammals but not NMRs could provide novel targets to slow or prevent cardiovascular aging in humans.

  18. O direito fundamental ao conteúdo do próprio trabalho: uma reconstrução normativa do direito ao trabalho

    OpenAIRE

    Wandelli, Leonardo Vieira

    2016-01-01

    Resumo: Pretende-se enfrentar a inefetividade do direito humano e fundamental ao trabalho a partir de um esforço de reconstrução normativa da sua fundamentação, apoiada em um duplo pilar teórico, nas teorias das necessidades e nas teorias do reconhecimento. Com isso, não somente se recupera a importância do vínculo entre trabalho e dignidade humana, como se explicitam aspectos essenciais da centralidade do trabalho para os sujeitos. A partir disso, o direito ao trabalho se desvela não apenas ...

  19. Preditores das atitudes negativas face ao envelhecimento e face à sexualidade na terceira idade

    OpenAIRE

    Pereira, Diane; Ponte, Filomena; Costa, Eleonora

    2018-01-01

    Este estudo analisou as diferenças entre jovens e idosos ao nível das atitudes e dos conhecimentos face ao envelhecimento e face à sexualidade na terceira idade, a relação entre as variáveis em estudo, e os preditores das atitudes negativas face ao envelhecimento e das atitudes negativas face à sexualidade na terceira idade. Cento e cinquenta e três jovens universitários e 42 idosos participaram no estudo. Os resultados indicaram que os jovens apresentam um menor nível de conhecimentos acerca...

  20. Comportamentos de adesão ao rastreio do cancro do colo do útero

    OpenAIRE

    Areias, Paula Alexandra Pombo Pereira

    2011-01-01

    Enquadramento: O cancro do colo do útero é o segundo tipo de cancro mais frequente na mulher, a adesão ao rastreio possibilita a identificação do problema numa fase precoce. Objectivos: O nosso estudo tem como objectivo analisar em que medida as características sóciodemográficas e profissionais, os conhecimentos em relação ao cancro do colo do útero e rastreio, as crenças de saúde afectam a adesão ao rastreio. Métodos: Realizamos um estudo de natureza quantitativa, não experime...

  1. Contribuição ao estudo da hipsarritmia

    Directory of Open Access Journals (Sweden)

    Ana Maria Fisch

    1966-03-01

    Full Text Available Resumindo as principais contribuições que resultaram na conceituação atual da hipsarritmia, destacam os autores a noção de que não constitui ela uma entidade clínica determinada, mas uma sindrome eletroclínica especial, própria da primeira infância, e cujo diagnóstico só pode ser firmado à luz do traçado eletrencefalográfico característico. Analisam a variedade de fatôres etiológicos à maneira do que ocorre com as encefalopatias infantis em geral, chamando a atenção para a importância do fator etário, das condições traumáticas e do problema da anóxia cerebral neonatorum. Necessária ênfase é conferida ao fenômeno da maturação encefálica que explica, segundo o consenso dos autores modernos, as características peculiares da síndrome. A hipsarritmia pode instalar-se em indivíduo previamente são ou em encefalopata, tendo evolução variável, pois tanto pode evolver favoràvelmente, o que é mais raro, como modificar-se, seja no sentido da epilepsia focal ou outra forma de epilepsia, o que é mais comum, ou, então, permanecer inalterada. Essa evolução - a história natural da hipsarritmia - é apreciada especialmente sob o aspecto eletrencefalográfico, o qual pode, ou não, ser acompanhado de correspondente evolução clínica. A sintomatologia é variável, sendo de notar a grande dominância das crises de espasmos em flexão. Sendo pobre o subsídio anátomo-patológico da condição, os autores, a êsse propósito, apenas acrescentam os dados referentes à necropsia de um dos seus casos, com achados inespecíficos. O prognóstico, na maioria das vêzes mau, pode variar, especialmente após o uso do ACTH. O tratamento é difícil, freqüentemente desapontador. As melhores respostas são obtidas com o ACTH e, algumas vêzes, com os antibióticos e medicação sintomática, porém, quase sempre reaparece a sintomatologia, se suprimida a medicação que, por outro lado, nem sempre pode ser continuada.

  2. Potencialidades e espaços ao empreendedorismo

    Directory of Open Access Journals (Sweden)

    Adriano Lago

    2008-01-01

    Full Text Available A criação de negócios é apontada como uma das causas da prosperidade de um local, região, estado ou de um país. É neste sentido que o empreendedorismo tem recebido destaque nos últimos anos, como promotor de novos negócios. Captar, descrever e analisar o fenômeno do empreendedorismo é, portanto, necessário para o desenho de ações de promoção do desenvolvimento. Assim, o presente estudo aborda o empreendedorismo na piscicultura, tomando como base três casos analisados: uma propriedade com rizipiscicultura, uma com piscicultura de ciclo completo inclusive com frigorífico, e outra onde a piscicultura desenvolve-se através de um pesque-pague, com vistas a proporcionar o turismo rural. Para fins de pesquisa, entrevistaram-se os proprietários de cada estabelecimento na busca dos dados necessários para a construção de uma abordagem consistente da temática proposta, ou seja, identificar a presença do empreendedorismo e suas características particulares em cada propriedade analisada. Verificou-se que determinadas características empreendedoras são comuns aos três casos analisados: habilidade para identificar oportunidades; busca de conhecimento de sua área de atuação; senso de organização; disposição para tomar decisões; capacidade de liderança; talento para empreender; otimismo e tino empresarial; ser estrategista, além do dinamismo. Portanto, foi possível observar que o perfil empreendedor dos proprietários de cada negócio tornou possível a transformação de idéias e problemas em oportunidades reais de negócio.Abstract The business opening is indicated as one of the prosperity causes of a country, state, region or local. It’s in this context that the entrepreneurship has received attention along the last years as new business promoter. This way, to study and promote development actions it’s necessary, firstly, to get, to describe and to analyze the entrepreneurship phenomenon by itself. So, this study

  3. Dynamic SPECT of the brain using a lipophilic technetium-99m complex, PnAO

    DEFF Research Database (Denmark)

    Holm, S; Andersen, A R; Vorstrup, S

    1985-01-01

    m PnAO was injected i.v. as a bolus of 15 to 25 mCi. The distribution was followed over 10-sec intervals using a highly sensitive, rapidly rotating SPECT (Tomomatic 64) and compared to 133Xe flow maps. Upon arrival of the PnAO bolus to the brain, a high uptake was found in brain tissue with high......The lipophilic 99mTc-labeled oxime propylene amine oxime (PnAO) should, according to recent reports behave like 133Xe in the human brain. This study compares SPECT images of the two tracers in six subjects: four stroke cases, one transitory ischemic attack case and one normal subject. Technetium-99......AO has a high yet incomplete brain extraction yielding a flow dominated initial distribution with limitations mentioned....

  4. Exame Nacional do Ensino Médio e acesso de estudantes surdos ao Ensino Superior Brasileiro

    Directory of Open Access Journals (Sweden)

    Diléia Ap. Martins

    2015-12-01

    Full Text Available Resumo Este trabalho apresenta as primeiras aproximações relativas à pesquisa "Acessibilidade e Exame Nacional do Ensino Médio: desafios para a escolarização de estudantes surdos", inserida no programa Observatório em Educação (Obeduc, Projeto Acessibilidade no Ensino Superior. Investiga dados oficiais publicados pelo Instituto Nacional de Estudos e Pesquisas (Inep, referentes ao aumento progressivo do número de matrículas de estudantes surdos em Instituições de Ensino Superior (IES. Analisa questões relativas à nota obtida por esses estudantes, ao se submeterem ao Exame Nacional do Ensino Médio (Enem, e o uso dessa nota para ingressarem em universidades brasileiras. Os dados analisados, no presente artigo, indicam discrepância de desempenho de estudantes sem deficiência, quando comparados aos estudantes surdos.

  5. Percepção do consumidor frente aos riscos associados aos alimentos, sua segurança e rastreabilidade

    Directory of Open Access Journals (Sweden)

    Juliana Cunha de Andrade

    2013-09-01

    mostrou relevante nos três grupos, pois esta deve contribuir para o recolhimento mais eficiente de produtos, caso seja necessário. No entanto, sob a ótica do consumidor, a rastreabilidade leva ao aumento dos preços, embora aumente a confiança em relação à segurança dos alimentos. Muitas das preocupações dos consumidores refletem as informações publicadas em revistas e jornais, como o caso da crença de que hormônios são utilizados na cadeia da carne de frango. Indústria e varejistas devem incrementar a comunicação de informações científicas sobre a segurança dos alimentos, contribuindo, assim, na educação do consumidor.

  6. Desempenho de vacas Charolês e Nelore desterneiradas aos três ou sete meses

    Directory of Open Access Journals (Sweden)

    Restle João

    2001-01-01

    Full Text Available Foi avaliado o desempenho de vacas Charolês (C e Nelore (N, agrupadas em três classes de idade, jovens (3 e 4 anos, adultas (5 a 7 anos e velhas (8 ou mais anos, desmamadas aos três (precoce ou sete meses no outono (tradicional. O peso no outono das vacas desterneiradas aos três meses (T3 foi 45 kg superior ao das vacas com remoção do bezerro aos sete meses (T7. O estado corporal aos sete meses também foi melhor nas vacas do T3 (3,3 contra 2,1 pontos. Vacas do T3 apresentaram maior ganho de peso do parto ao final do período reprodutivo e apresentaram maiores porcentagem de cio (81 contra 51% e prenhez (67,2 contra 37,3% e menor intervalo do parto ao primeiro cio pós-parto (102 contra 114 dias que vacas do T7. Vacas adultas apresentaram melhor estado corporal aos sete meses e tiveram melhor desempenho reprodutivo do que vacas velhas e jovens. A diferença na porcentagem de prenhez entre o T3 e T7 foi mais evidente nas vacas jovens (42,11 contra 12,5% e velhas (51,72 contra 35,71% que nas adultas (62,50 contra 53,33%. Vacas C foram mais pesadas que as N, ao parto, aos três e sete meses pós-parto e apresentaram melhor estado corporal aos sete meses. O efeito do desmame precoce no desempenho reprodutivo foi mais evidente nas vacas C. A porcentagem de fêmeas prenhes nas C foi de 80,60% para o T3 e 41,90% para o T7, já nas N as porcentagens foram de 45,50 e 30,00%, respectivamente, para o T3 e T7. Nas vacas C, a produção de leite e a amamentação apresentaram efeito inibidor, sobre a reprodução, mais marcante que nas vacas N.

  7. Attitude toward contraception and abortion among Curaçao women. Ineffective contraception due to limited sexual education?

    NARCIS (Netherlands)

    Brink, M.J.; Boersma, A.A.; Meyboom-deJong, B; de Bruijn, J.G.M.

    2011-01-01

    Background: In Curaçao is a high incidence of unintended pregnancies and induced abortions. Most of the induced abortions in Curaçao are on request of the woman and performed by general practitioners. In Curaçao, induced abortion is strictly prohibited, but since 1999 there has been a policy of

  8. Safety evaluation of pectin-derived acidic oligosaccharides (pAOS): genotoxicity and sub-chronic studies.

    NARCIS (Netherlands)

    Garthoff, J.A.; Heemskerk, S.; Hempenius, R.A.; Lina, B.A.; Krul, C.A.; Koeman, J.H.; Speijers, G.J.

    2010-01-01

    Pectin-derived acidic oligosaccharides (pAOS) are non-digestible carbohydrates to be used in infant formulae and medical nutrition. To support its safety, the genotoxic potential of pAOS was evaluated. pAOS was not mutagenic in the Ames test. Positive results were obtained in the chromosome

  9. Safety evaluation of pectin-derived acidic oligosaccharides (pAOS): Genotoxicity and sub-chronic studies

    NARCIS (Netherlands)

    Garthoff, J.A.; Heemskerk, S.; Hempenius, R.A.; Lina, B.A.R.; Krul, C.A.M.; Koeman, J.H.; Speijers, G.J.A.

    2010-01-01

    Pectin-derived acidic oligosaccharides (pAOS) are non-digestible carbohydrates to be used in infant formulae and medical nutrition. To support its safety, the genotoxic potential of pAOS was evaluated. pAOS was not mutagenic in the Ames test. Positive results were obtained in the chromosome

  10. OPERA, an automatic PSF reconstruction software for Shack-Hartmann AO systems: application to Altair

    Science.gov (United States)

    Jolissaint, Laurent; Veran, Jean-Pierre; Marino, Jose

    2004-10-01

    When doing high angular resolution imaging with adaptive optics (AO), it is of crucial importance to have an accurate knowledge of the point spread function associated with each observation. Applications are numerous: image contrast enhancement by deconvolution, improved photometry and astrometry, as well as real time AO performance evaluation. In this paper, we present our work on automatic PSF reconstruction based on control loop data, acquired simultaneously with the observation. This problem has already been solved for curvature AO systems. To adapt this method to another type of WFS, a specific analytical noise propagation model must be established. For the Shack-Hartmann WFS, we are able to derive a very accurate estimate of the noise on each slope measurement, based on the covariances of the WFS CCD pixel values in the corresponding sub-aperture. These covariances can be either derived off-line from telemetry data, or calculated by the AO computer during the acquisition. We present improved methods to determine 1) r0 from the DM drive commands, which includes an estimation of the outer scale L0 2) the contribution of the high spatial frequency component of the turbulent phase, which is not corrected by the AO system and is scaled by r0. This new method has been implemented in an IDL-based software called OPERA (Performance of Adaptive Optics). We have tested OPERA on Altair, the recently commissioned Gemini-North AO system, and present our preliminary results. We also summarize the AO data required to run OPERA on any other AO system.

  11. East Asian winter temperature variation associated with the combined effects of AO and WP pattern

    Science.gov (United States)

    Park, Hye-Jin; Ahn, Joong-Bae

    2016-04-01

    The combined effects of the Arctic Oscillation (AO) and Western Pacific (WP) teleconnection pattern on the East Asian winter monsoon (EAWM) over the last 56 years (1958/59-2013/2014) were investigated using NCEP/NCAR reanalysis data (Park and Ahn, 2015). The study results revealed that the effect of the AO on winter temperature in East Asia could be changed depending on the phases of the WP pattern in the North Pacific. The negative relationship between the EAWM and the AO increased when the AO and WP were in-phase with each other. Hence, when winter negative (positive) AO was accompanied by negative (positive) WP, negative (positive) temperature anomalies were dominant across the entire East Asia region. Conversely, when the AO and WP were of-of-phase, the winter temperature anomaly in East Asia did not show distinct changes. Furthermore, from the perspective of stationary planetary waves, the zonal wavenumber-2 patterns of sea level pressure and geopotential height at 500hPa circulation strengthened when the AO and WP were in-phase but were not significant for the out-of-phase condition. It explained the possible mechanism of the combined effects of the AO and WP on the circulation related to EAWM. Reference Park, H.-J., and J.-B. Ahn (2015) Combined effect of the Arctic Oscillation and the Western Pacific pattern on East Asia winter temperature, Clim. Dyn. DOI:10.1007/s00382-015-2763-2. Acknowledgements This work was funded by the Korea Meteorological Administration Research and Development Program under grant KMIPA2015-2081.

  12. Functional analysis of AoAtg11 in selective autophagy in the filamentous fungus Aspergillus oryzae.

    Science.gov (United States)

    Tadokoro, Takayuki; Kikuma, Takashi; Kitamoto, Katsuhiko

    2015-07-01

    Autophagy is a highly conserved cellular degradation process in eukaryotes and consists of both non-selective and selective types. Selective autophagic processes include pexophagy, mitophagy, and the cytoplasm-to-vacuole targeting (Cvt) pathway of yeast, in which particular vacuolar proteins, such as aminopeptidase I (Ape1), are selectively transported to vacuoles. Although selective autophagy has been mainly studied in the yeasts Saccharomyces cerevisiae and Pichia pastoris, there is evidence for selective autophagy in filamentous fungi; however, the details are poorly understood. In S. cerevisiae, Atg11 is a selective autophagy-specific protein that recognizes and transports substrates to the pre-autophagosomal structure (PAS). Here, we first identified an ATG11 homologue in the filamentous fungus Aspergillus oryzae and analyzed the localization of the corresponding protein, designated AoAtg11, fused to enhanced green fluorescent protein (EGFP). Imaging analysis revealed that AoAtg11-EGFP was localized to PAS-like structures. We next constructed an Aoatg11 disruptant of A. oryzae and showed that AoAtg11 is involved in pexophagy and mitophagy. In addition, AoAtg11 was found to be dispensable for non-selective autophagy and for transporting AoApe1 to vacuoles. Taken together, these results suggest that AoAtg11 is a selective autophagy-specific protein in A. oryzae, and has distinct molecular functions from that of S. cerevisiae Atg11. Copyright © 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  13. Surface and bulk modifications of 1.5 mole % Sr/La{sub 2}O{sub 3} catalyst with different Co, Ni, and Cu amounts

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez Cortes, Sergio; Fontal, Bernardo [Universidad de los Andes, Merida (Venezuela); Moronta, Delfin [Universidad Central de Venezuela, Caracas (Venezuela)

    2001-08-01

    The effects of Co, Ni and Cu oxide content on the surface and bulk of the 1.5 mole % Sr/La{sub 2}O{sub 3} catalysts were studied. Series of M/Sr-La catalysts (MCo, Ni, Cu) were prepared by successive immersion impregnation. The solids were characterized by X-ray diffraction (XRD), infrared spectroscopy (FTIR), temperature-programmed reduction (TPR), electron paramagnetic resonance (EPR), specific surface area measurements (BET) and thermogravimetric analysis (TGA). The Sr/La{sub 2}O{sub 3} modified with different Co, Ni, and Cu loading shows a strong metal oxide interaction with La{sub 2}O{sub 3}. This probably begin over anionic vacancies or structure defects favoring LaSrMO{sub x} solid solution formation with island-like structures according to a Volmer-Weber growth. It is proposed that the growth mode of La-Co, La-Ni and La-Cu stoichiometric phases occur from solid solutions. The formation of only a La-M single phase, probably non-stoichiometric, increases the material porosity while different phases favor the agglomeration or sintering of the particles. [Spanish] En este trabajo se estudia el efecto de la cantidad de cobalto, niquel y cobre sobre las estructuras superficial y masica del sistema 1.5% molar Sr/La{sub 2}O{sub 3}. Los catalizadores M/Sr-La (M = Co, Ni, Cu) fueron preparados por el metodo de impregnacion con exceso de solucion. Los solidos se caracterizaron por difraccion de rayos-X, espectroscopia infrarroja con transformada de Fourier, reduccion a temperatura programada, resonancia paramagnetica del electron, medidas del area superficial especifica por el metodo de BET y analisis termogravimetrico. El catalizador Sr/La{sub 2}O{sub 3} modificado con diferentes cantidades de Co, Ni y Cu muestra una interaccion fuerte con el oxido del metal de transicion, probablemente sobre las vacancias anionicas o defectos estructurales que favorecen la formacion de la solucion solida LaSrMox en forma de isla, segun el mecanismo de crecimiento de fase de Volmer

  14. Estimating surface CO2 fluxes from space-borne CO2 dry air mole fraction observations using an ensemble Kalman Filter

    Directory of Open Access Journals (Sweden)

    S. Dance

    2009-04-01

    Full Text Available We have developed an ensemble Kalman Filter (EnKF to estimate 8-day regional surface fluxes of CO2 from space-borne CO2 dry-air mole fraction observations (XCO2 and evaluate the approach using a series of synthetic experiments, in preparation for data from the NASA Orbiting Carbon Observatory (OCO. The 32-day duty cycle of OCO alternates every 16 days between nadir and glint measurements of backscattered solar radiation at short-wave infrared wavelengths. The EnKF uses an ensemble of states to represent the error covariances to estimate 8-day CO2 surface fluxes over 144 geographical regions. We use a 12×8-day lag window, recognising that XCO2 measurements include surface flux information from prior time windows. The observation operator that relates surface CO2 fluxes to atmospheric distributions of XCO2 includes: a the GEOS-Chem transport model that relates surface fluxes to global 3-D distributions of CO2 concentrations, which are sampled at the time and location of OCO measurements that are cloud-free and have aerosol optical depths 2 profiles to XCO2, accounting for differences between nadir and glint measurements, and the associated scene-dependent observation errors. We show that OCO XCO2 measurements significantly reduce the uncertainties of surface CO2 flux estimates. Glint measurements are generally better at constraining ocean CO2 flux estimates. Nadir XCO2 measurements over the terrestrial tropics are sparse throughout the year because of either clouds or smoke. Glint measurements provide the most effective constraint for estimating tropical terrestrial CO2 fluxes by accurately sampling fresh continental outflow over neighbouring oceans. We also present results from sensitivity experiments that investigate how flux estimates change with 1 bias and unbiased errors, 2 alternative duty cycles, 3 measurement density and correlations, 4 the spatial resolution of estimated flux estimates, and 5 reducing the length of the lag window and the

  15. Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma.

    Science.gov (United States)

    Thieme, René; Kurz, Susanne; Kolb, Marlen; Debebe, Tewodros; Holtze, Susanne; Morhart, Michaela; Huse, Klaus; Szafranski, Karol; Platzer, Matthias; Hildebrandt, Thomas B; Birkenmeier, Gerd

    2015-01-01

    The naked mole-rat (NMR) is a long-lived and cancer resistant species. Identification of potential anti-cancer and age related mechanisms is of great interest and makes this species eminent to investigate anti-cancer strategies and understand aging mechanisms. Since it is known that the NMR expresses higher liver mRNA-levels of alpha 2-macroglobulin than mice, nothing is known about its structure, functionality or expression level in the NMR compared to the human A2M. Here we show a comprehensive analysis of NMR- and human plasma-A2M, showing a different prediction in glycosylation of NMR-A2M, which results in a higher molecular weight compared to human A2M. Additionally, we found a higher concentration of A2M (8.3±0.44 mg/mL vs. and 4.4±0.20 mg/mL) and a lower total plasma protein content (38.7±1.79 mg/mL vs. 61.7±3.20 mg/mL) in NMR compared to human. NMR-A2M can be transformed by methylamine and trypsin resulting in a conformational change similar to human A2M. NMR-A2M is detectable by a polyclonal antibody against human A2M. Determination of tryptic and anti-tryptic activity of NMR and human plasma revealed a higher anti-tryptic activity of the NMR plasma. On the other hand, less proteolytic activity was found in NMR plasma compared to human plasma. We found transformed NMR-A2M binding to its specific receptor LRP1. We could demonstrate lower protein expression of LRP1 in the NMR liver tissue compared to human but higher expression of A2M. This was accompanied by a higher EpCAM protein expression as central adhesion molecule in cancer progression. NMR-plasma was capable to increase the adhesion in human fibroblast in vitro most probably by increasing CD29 protein expression. This is the first report, demonstrating similarities as well as distinct differences between A2M in NMR and human plasma. This might be directly linked to the intriguing phenotype of the NMR and suggests that A2M might probably play an important role in anti-cancer and the anti

  16. Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma.

    Directory of Open Access Journals (Sweden)

    René Thieme

    Full Text Available The naked mole-rat (NMR is a long-lived and cancer resistant species. Identification of potential anti-cancer and age related mechanisms is of great interest and makes this species eminent to investigate anti-cancer strategies and understand aging mechanisms. Since it is known that the NMR expresses higher liver mRNA-levels of alpha 2-macroglobulin than mice, nothing is known about its structure, functionality or expression level in the NMR compared to the human A2M.Here we show a comprehensive analysis of NMR- and human plasma-A2M, showing a different prediction in glycosylation of NMR-A2M, which results in a higher molecular weight compared to human A2M. Additionally, we found a higher concentration of A2M (8.3±0.44 mg/mL vs. and 4.4±0.20 mg/mL and a lower total plasma protein content (38.7±1.79 mg/mL vs. 61.7±3.20 mg/mL in NMR compared to human. NMR-A2M can be transformed by methylamine and trypsin resulting in a conformational change similar to human A2M. NMR-A2M is detectable by a polyclonal antibody against human A2M. Determination of tryptic and anti-tryptic activity of NMR and human plasma revealed a higher anti-tryptic activity of the NMR plasma. On the other hand, less proteolytic activity was found in NMR plasma compared to human plasma.We found transformed NMR-A2M binding to its specific receptor LRP1. We could demonstrate lower protein expression of LRP1 in the NMR liver tissue compared to human but higher expression of A2M. This was accompanied by a higher EpCAM protein expression as central adhesion molecule in cancer progression. NMR-plasma was capable to increase the adhesion in human fibroblast in vitro most probably by increasing CD29 protein expression. This is the first report, demonstrating similarities as well as distinct differences between A2M in NMR and human plasma. This might be directly linked to the intriguing phenotype of the NMR and suggests that A2M might probably play an important role in anti-cancer and the

  17. Instrumento mensurador de adesao para hipertensos: contribuicao da Teoria da Resposta ao Item

    Directory of Open Access Journals (Sweden)

    Malvina Thais Pacheco Rodrigues

    2013-06-01

    Full Text Available OBJETIVO: Analisar instrumento de medição da adesão ao tratamento da hipertensão por meio da "Teoria da Resposta ao Item". MÉTODOS: Estudo analítico com 406 hipertensos com complicações associadas, atendidos na atenção básica em Fortaleza, CE, 2011, pela "Teoria da Resposta ao Item". As etapas de execução foram: teste de dimensionalidade, calibração dos itens; tratamento dos dados e construção da escala, analisadas com base no modelo de resposta gradual. Estudo da dimensionalidade do instrumento foi feito pela análise da matriz de correlação policórica e análise fatorial de informação completa. Utilizou-se o software Multilog para calibração dos itens e estimação dos escores. RESULTADOS: Os itens referentes ao tratamento medicamentoso foram os mais diretamente relacionados à adesão, enquanto aqueles referentes ao tratamento não medicamentoso precisam ser reformulados, pois possuíram menor quantidade de informação psicométrica e baixa discriminação. A independência dos itens, o reduzido número de níveis da escala e as baixas variâncias explicadas no ajuste dos modelos mostraram as principais fragilidades do instrumento analisado. A "Teoria da Resposta ao Item" mostrou-se relevante para análise, pois avaliou o respondente quanto à adesão ao tratamento da hipertensão, ao nível de dificuldade dos itens e à sua capacidade de discriminação entre indivíduos com diferentes níveis de adesão, o que gerou maior quantidade de informação. CONCLUSÕES: O instrumento analisado é limitado para medir a adesão ao tratamento da hipertensão, mediante análise pela "Teoria da Resposta ao Item", e necessita de ajustes. A adequada formulação dos itens é importante para medir precisamente o traço latente desejado.

  18. O processo de qualificação dos ovos moles de Aveiro: indicação geográfica protegida

    OpenAIRE

    Barros, Patrícia Cláudia Cardoso Teixeira Freitas da Naia

    2010-01-01

    Os produtos tradicionais possuem características únicas, diferenciadoras, ligadas à sua região de origem e ao saber fazer, que são determinantes para as suas qualidades intrínsecas e notoriedade. O Regulamento (CE) n.º 510/2006 prevê a protecção comunitária das denominações dos produtos agrícolas e dos géneros alimentícios que têm uma relação estreita com a sua região geográfica de produção. Dependendo das especificidades dos produtos e da sua ligação à origem, os nomes dos produtos tradicion...

  19. Characterization of gonadotrophin-releasing hormone precursor cDNA in the Old World mole-rat Cryptomys hottentotus pretoriae: high degree of identity with the New World guinea pig sequence.

    Science.gov (United States)

    Kalamatianos, T; du Toit, L; Hrabovszky, E; Kalló, I; Marsh, P J; Bennett, N C; Coen, C W

    2005-05-01

    Regulation of pituitary gonadotrophins by the decapeptide gonadotrophin-releasing hormone 1 (GnRH1) is crucial for the development and maintenance of reproductive functions. A common amino acid sequence for this decapeptide, designated as 'mammalian' GnRH, has been identified in all mammals thus far investigated with the exception of the guinea pig, in which there are two amino acid substitutions. Among hystricognath rodents, the members of the family Bathyergidae regulate reproduction in response to diverse cues. Thus, highveld mole-rats (Cryptomys hottentotus pretoriae) are social bathyergids in which breeding is restricted to a particular season in the dominant female, but continuously suppressed in subordinate colony members. Elucidation of reproductive control in these animals will be facilitated by characterization of their GnRH1 gene. A partial sequence of GnRH1 precursor cDNA was isolated and characterized. Comparative analysis revealed the highest degree of identity (86%) to guinea pig GnRH1 precursor mRNA. Nevertheless, the deduced amino acid sequence of the mole-rat decapeptide is identical to the 'mammalian' sequence rather than that of guinea pigs. Successful detection of GnRH1-synthesizing neurones using either a guinea pig GnRH1 riboprobe or an antibody against the 'mammalian' decapeptide is consistent with the guinea pig-like sequence for the precursor and the classic 'mammalian' form for the decapeptide. The high degree of identity in the GnRH1 precursor sequence between this Old World mole-rat and the New World guinea pig is consistent with the theory that caviomorphs and phiomorphs originated from a common ancestral line in the Palaeocene to mid Eocene, some 63-45 million years ago.

  20. Potential use of the non-random distribution of N2 and N2O mole masses in the atmosphere as a tool for tracing atmospheric mixing and isotope fractionation processes

    International Nuclear Information System (INIS)

    Well, R.; Langel, R.; Reineking, A.

    2002-01-01

    The variation in the natural abundance of 15 N in atmospheric gas species is often used to determine the mixing of trace gases from different sources. With conventional budget calculations one unknown quantity can be determined if the remaining quantities are known. From 15 N tracer studies in soils with highly enriched 15 N-nitrate a procedure is known to calculate the mixing of atmospheric and soil derived N 2 based on the measurement of the 30/28 and 29/28 ratios in gas samples collected from soil covers. Because of the non-random distribution of the mole masses 30 N 2 , 29 N 2 and 28 N 2 in the mixing gas it is possible to calculate two quantities simultaneously, i.e. the mixing ratio of atmospheric and soil derived N 2 , and the isotopic signature of the soil derived N 2 . Routine standard measurements of laboratory air had suggested a non-random distribution of N 2 -mole masses. The objective of this study was to investigate and explain the existence of non-random distributions of 15 N 15 N, 14 N 15 N and 14 N 14 N in N 2 and N 2 O in environmental samples. The calculation of theoretical isotope data resulting from hypothetical mixing of two sources differing in 15 N natural abundance demonstrated, that the deviation from an ideal random distribution of mole masses is not detectable with the current precision of mass spectrometry. 15 N-analysis of N 2 or N 2 O was conducted with randomised and non-randomised replicate samples of different origin. 15 N abundance as calculated from 29/28 ratios were generally higher in randomised samples. The differences between the treatments ranged between 0.05 and 0.17 δper mille 15 N. It was concluded that the observed randomisation effect is probably caused by 15 N 15 N fractionation during environmental processes. (author)

  1. Morfologia e Crescimento dos 6 aos 10 anos de idade em Viana do Castelo, Portugal

    Directory of Open Access Journals (Sweden)

    Luis Paulo Rodrigues

    2008-09-01

    Full Text Available Ao longo de quatro anos, o Estudo Morfofuncional da Criança Vianense observou 1911 crianças entre os 6e os 10 anos de idade, resultando em 4064 observações individuais(2054 de raparigas, 2006 de rapazes.Os resultados encontrados nos indicadores morfológicos simples(altura, peso, pregas adiposas, diâmetros ósseos e perímetros muscularese no somatótipo, são descritos normativamente (valores percentílicose comparados com outros estudos nacionais e internacionais. As crianças vianenses demonstraram possuir uma estatura média ligeiramente superior às reportadas nos estudos portugueses e um ritmo de crescimento diferente das norte-americanas; peso semelhante ao das congéneres nacionais mas inferior ao das EUA; valores de pregas adiposas geralmente inferiores aos encontrados em Portugal e EUA; perímetros musculares e diâmetros ósseos semelhantes aos seus pares portugueses; e uma tendência para o aumento, com a idade, do ectomorfismo nos rapazes e do endomorfismo em ambos os sexos. Este panorama parece indicar que as crianças vianenses apresentam características de aptidão morfologia que estão longe de espelhar as preocupações internacionais nesta matéria.

  2. Control rod drop accident analysis for the mixed core project in Ling Ao NPS

    International Nuclear Information System (INIS)

    Zhang Shishun; Zhou Zhou; Xiao Min

    2004-01-01

    AFA-2G assemblies in Ling Ao NPS (LNPS) have been replaced gradually by AFA-3G assemblies from cycle 2 and subsequent cycles. the enrichment of the fuels will be increased from 3.2% to 3.7% from cycle 3 in Ling Ao. Therefore, the study of ling Ao mixed core and increased enrichment have been performed since 2001. Lots of accidents need to be re-analyzed in Ling Ao NPS in order to verify its safety requirements for the new fuel management. Control rod drop accident for LNPS was re-analyzed in 2001 in frame of FRAMATOME ANP analytical methodology. The analytical codes used in the accident analysis include SCIENCE, ESPADON, CINEMA, CANTAL and FLICA III. The control rod drop accident analysis is performed with respect to the 10 reference cycles of the generic fuel management design for Ling Ao mixed core and increased enrichment study. The pre-drop FδH for the first transition cycles and other cycles are 1.52 and 1.55, respectively. For detected dropped rod configurations, the negative flux rate protection system actuates a reactor trip. For the non-detected dropped rod configurations, the minimum DNBR values have been evaluated with conservative analysis methodology and assumptions and the DNBR fuel design limit is respected the analytical results shows that, for all the non-detected dropped rod configurations, the minimum DNB margin is about 2% which occurs in AFA-2G fuel assembly in the first transition cycle. (author)

  3. Requirements Modeling with the Aspect-oriented User Requirements Notation (AoURN): A Case Study

    Science.gov (United States)

    Mussbacher, Gunter; Amyot, Daniel; Araújo, João; Moreira, Ana

    The User Requirements Notation (URN) is a recent ITU-T standard that supports requirements engineering activities. The Aspect-oriented URN (AoURN) adds aspect-oriented concepts to URN, creating a unified framework that allows for scenario-based, goal-oriented, and aspect-oriented modeling. AoURN is applied to the car crash crisis management system (CCCMS), modeling its functional and non-functional requirements (NFRs). AoURN generally models all use cases, NFRs, and stakeholders as individual concerns and provides general guidelines for concern identification. AoURN handles interactions between concerns, capturing their dependencies and conflicts as well as the resolutions. We present a qualitative comparison of aspect-oriented techniques for scenario-based and goal-oriented requirements engineering. An evaluation carried out based on the metrics adapted from literature and a task-based evaluation suggest that AoURN models are more scalable than URN models and exhibit better modularity, reusability, and maintainability.

  4. Adesão de pessoas com diabetes mellitus tipo 2 ao tratamento medicamentoso

    Directory of Open Access Journals (Sweden)

    Lilian Cristiane Gomes-Villas Boas

    2014-04-01

    Full Text Available Estudo seccional de abordagem quantitativa que objetivou avaliar a adesão ao tratamento medicamentoso entre pessoas com diabetes mellitus, e sua relação com variáveis clínicas, de tratamento e controle metabólico. A amostra constituiu-se de 162 pessoas com diabetes mellitus tipo 2 em seguimento ambulatorial. Para a coleta dos dados, foram utilizadas a Medida de Adesão aos Tratamentos e a consulta aos prontuários dos participantes. Obteve-se elevada adesão ao tratamento medicamentoso. Para um p<0,05, obteve-se correlação inversa com a pressão arterial diastólica e correlação direta com a frequência diária de administração de insulina e de antidiabéticos orais. Não houve correlações estatisticamente significantes entre adesão e variáveis de controle metabólico. Os resultados divergem da literatura no que se refere à taxa de adesão ao tratamento medicamentoso em doenças crônicas, bem como na correlação entre adesão e complexidade do regime medicamentoso, o que aponta para a necessidade de mais estudos sobre essa temática.

  5. Photoluminescent properties of Pr3+ doped YTiAO6 (A= Nb&Ta) euxenite compounds

    Science.gov (United States)

    Venugopal, Meenu; Kumar, H. Padma

    2018-04-01

    Pr3+ doped YTiAO6 (A = Nb and Ta) compounds were prepared by conventional solid state ceramic route. X - ray diffraction studies of the samples confirmed the euxenite orthorhombic phase formation. Effect of Pr3+ doping on the optical and luminescence properties of YTiAO6 (A = Nb and Ta) were studied. The strong absorption in the UV region are due to the absorption by the host YTiAO6 (A = Nb and Ta) and all other peaks can be attributed to that of Pr3+. Host emission peaks are observed around 485 nm and 460 nm in all samples. The red emission observed at 614 nm in the doped samples corresponds to the 1D2 to 3H4 transition of Pr3+ ion. CIE colour coordinates and chromaticity values were also discussed.

  6. A Arqueologia do cuidado: da prática ao discurso

    Directory of Open Access Journals (Sweden)

    Sidi Askofare

    2006-06-01

    Full Text Available A noção de cuidado, assimilada há muito tempo ao tratamento e, então, ao conceito de terapêutica, retorna, logo depois, nos estudos, pesquisas e reflexões nas ciências humanas. Mas, daí em diante, está emancipado de suas aderências e conotações médicas por serem demasiadamente redutoras. O presente artigo propõe uma arqueologia (no sentido foucaultiano do cuidado &– de suas origens animais às suas formas culturalmente e historicamente definidas &– e põe em evidência a subversão que produz no campo do cuidado &– das práticas aos discursos &– a emergência e a dominação do discurso da ciência.

  7. Obtenção de plantas de feijão-caupi resistentes ao Cowpea severe mosaic virus e ao Cowpea aphid-borne mosaic virus

    Directory of Open Access Journals (Sweden)

    Gislanne Brito Barros

    2013-06-01

    Full Text Available Dentre os vírus que infectam o feijão-caupi (Vigna unguiculata L. Walp. destacam-se, respectivamente, pela severidade e ampla ocorrência o Cowpea severe mosaic virus (CPSMV e o Cowpea aphid-borne mosaic virus (CABMV. Portanto, objetivaram-se, no presente trabalho, obter e avaliar plantas de feijão-caupi com resistência ao CPSMV e ao CABMV, visando ao desenvolvimento de cultivares essencialmente derivadas e novas cultivares. Realizaram-se oito cruzamentos seguidos de retrocruzamentos, utilizando a linhagem TE 97-309G-9 e a cultivar Patativa como genitores resistentes, e as cultivares BR3-Tracuateua, BRS-Urubuquara, BRS-Novaera, BRS-Guariba e Pretinho como genitores suscetíveis. As gerações F2 e F2RC1 foram desafiadas quanto à resistência por meio de inoculação mecânica com isolados do CPSMV e do CABMV. Nas gerações F2RC1, além da resistência foram avaliados os caracteres: número de dias para o início da floração, comprimento das vagens, número de grãos. vagem-1, peso de cem grãos e produção de grãos.planta-1. Todos os indivíduos F2 e F2RC1 foram analisados pelo teste χ² e se ajustaram à frequência esperada de 15 plantas suscetíveis 1 planta resistente a ambos os vírus. As médias das plantas F2RC1 resistentes, de cada retrocruzamento, foram comparadas com a média do seu respectivo genitor recorrente pelo teste 't' e as médias dos retrocruzamentos foram comparadas pelo teste de Scott-Knott. Foi detectada variabilidade genética entre os retrocruzamentos para todos os caracteres. Todos os retrocruzamentos foram considerados promissores para produção de cultivares essencialmente derivadas resistentes ao CPSMV e ao CABMV e as plantas selecionadas possuem características que possibilitam a seleção de linhagens com grãos de bom padrão comercial e altamente produtivas.

  8. Facilitadores e barreiras de retorno ao trabalho de trabalhadores acometidos por LER/DORT

    Directory of Open Access Journals (Sweden)

    Jorge Henrique Santos Saldanha

    Full Text Available INTRODUÇÃO: As Lesões por Esforços Repetitivos/Distúrbios Osteomusculares Relacionados ao Trabalho (LER/DORT causam afastamento do trabalho de uma grande parcela de trabalhadores. OBJETIVO: Entender e sistematizar de que forma os fatores relacionados ao retorno ao trabalho (RT de trabalhadores acometidos por LER/DORT são descritos na literatura como facilitadores ou barreiras. MÉTODOS: Revisão crítica de literatura do período de janeiro de 2005 a outubro de 2010 disponível nas bases Lilacs, MedLine e SciELO. As informações foram sistematizadas em três categorias de fatores que influenciam o RT e como facilitadores ou barreiras - de ordem pessoal, de ordem organizacional e relacionadas às características dos serviços, sistemas e políticas. RESULTADOS: Dos 633 artigos inicialmente levantados, 21 atenderam aos critérios de inclusão. Fatores identificados como mais relevantes: de ordem pessoal - dor, fatores psicossociais, afastamento do trabalho; organizacionais - modificações nos postos de trabalho, demanda psicológica do trabalho, apoio organizacional, apoio de colegas e da chefia; relacionados aos serviços, sistemas e políticas - programa de retorno ao trabalho, tempo de afastamento e serviços de apoio. CONCLUSÃO: O estudo evidenciou que o RT é um processo complexo, que demanda políticas intersetoriais, cooperação técnica e pactuação de metas entre os atores envolvidos no processo.

  9. Adesão ao Tratamento em Gestação de Alto Risco

    Directory of Open Access Journals (Sweden)

    Fabíola Langaro

    Full Text Available No Brasil, aproximadamente 15% das gestações são de alto risco, sendo os diagnósticos de diabetes gestacional e hipertensão as causas mais frequentes dessa condição. Para a efetividade dos tratamentos indicados nesses casos, bem como alcance de desfechos favoráveis, é essencial a adesão da paciente às recomendações fornecidas pelas equipes de saúde, o que torna fundamental a avaliação desse indicador. A pesquisa apresentada avaliou a adesão de participantes de um programa de atendimento interdisciplinar a gestantes de alto risco desenvolvido em um hospital geral privado por meio de três instrumentos: Questionário Morisky-Green de adesão ao uso de medicação, Questionário de Adesão ao Tratamento e Pergunta descritiva de avaliação qualitativa. Durante oito meses, 83 gestantes responderam aos questionários, que possibilitaram identificar níveis de adesão ao longo do tempo de participação no programa, situações de não adesão mais frequentes, bem como variáveis que interferem nas medidas de adesão ao uso de medicação e ao tratamento global. Considera-se que os índices levantados são passíveis de uso como indicadores clínicos e gerenciais, possibilitando a comprovação da efetividade e melhoria constante das rotinas de saúde. Tais mecanismos são úteis para definição de protocolos, discussão de casos clínicos e mesmo para feedbackàs próprias pacientes.

  10. Genes regulated by AoXlnR, the xylanolytic and cellulolytic transcriptional regulator, in Aspergillus oryzae.

    Science.gov (United States)

    Noguchi, Yuji; Sano, Motoaki; Kanamaru, Kyoko; Ko, Taro; Takeuchi, Michio; Kato, Masashi; Kobayashi, Tetsuo

    2009-11-01

    XlnR is a Zn(II)2Cys6 transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Overexpression of the aoxlnR gene in Aspergillus oryzae (A. oryzae xlnR gene) resulted in elevated xylanolytic and cellulolytic activities in the culture supernatant, in which nearly 40 secreted proteins were detected by two-dimensional electrophoresis. DNA microarray analysis to identify the transcriptional targets of AoXlnR led to the identification of 75 genes that showed more than fivefold increase in their expression in the AoXlnR overproducer than in the disruptant. Of these, 32 genes were predicted to encode a glycoside hydrolase, highlighting the biotechnological importance of AoXlnR in biomass degradation. The 75 genes included the genes previously identified as AoXlnR targets (xynF1, xynF3, xynG2, xylA, celA, celB, celC, and celD). Thirty-six genes were predicted to be extracellular, which was consistent with the number of proteins secreted, and 61 genes possessed putative XlnR-binding sites (5'-GGCTAA-3', 5'-GGCTAG-3', and 5'-GGCTGA-3') in their promoter regions. Functional annotation of the genes revealed that AoXlnR regulated the expression of hydrolytic genes for degradation of beta-1,4-xylan, arabinoxylan, cellulose, and xyloglucan and of catabolic genes for the conversion of D-xylose to xylulose-5-phosphate. In addition, genes encoding glucose-6-phosphate 1-dehydrogenase and L-arabinitol-4- dehydrogenase involved in D-glucose and L-arabinose catabolism also appeared to be targets of AoXlnR.

  11. Vulnerabilidade de adolescentes ao HIV/AIDS: revisão integrativa

    OpenAIRE

    Melina Mafra Toledo

    2008-01-01

    Introdução: A adolescência é um dos períodos mais intensos da vida, pelos desafios, descobertas e oportunidades de exploração nela presentes e, por isso, se constitui um determinante da vulnerabilidade ao HIV/AIDS. Objetivo: identificar as evidências científicas da literatura sobre os elementos da vulnerabilidade de adolescentes ao HIV/AIDS. Metodologia: Revisão sistemática da literatura, na modalidade de denominada revisão integrativa. A busca de dados foi realizada nas seguintes bases e ban...

  12. Imunidade celular em caninos neonatos - do nascimento ao 45° dia de idade

    Directory of Open Access Journals (Sweden)

    R.P. Klein

    2014-06-01

    Full Text Available O objetivo do presente trabalho foi acompanhar o desenvolvimento imunológico dos neonatos caninos, a fim de avaliar a imunidade celular pela análise dos leucócitos e linfócitos totais e das subpopulações de linfócitos T (CD4+ e CD8+ pela técnica de citometria de fluxo. Foram utilizados 30 cães neonatos de ambos os sexos, sem raça definida, aos três, 10, 17, 24, 31, 38 e 45 dias de idade. A contagem de leucócitos totais aos 45 dias (11.639±3.574 foi significativamente maior que no terceiro dia de idade (8.740±1.812 (P<0,05; não houve diferença entre a contagem total de linfócitos aos 45 dias em relação ao terceiro dia de idade. Quanto às subpopulações de LT CD4+ e LT CD8+, os percentuais de LT CD4+, aos três dias de idade (24,9±16,8%, foram inferiores quando comparados à média entre o 10°, o 24° e o 31°dia (35,5%, e os de CD8+, ao terceiro dia, menores em relação às médias do 10° e do 31° dia de idade. Pode-se concluir que as subpopulações de LT CD4+ e CD8+ sofrem oscilações durante o desenvolvimento pós-natal, sendo estas crescentes em relação aos níveis obtidos aos três dias de idade. A relação CD4+:CD8+ mostrou superioridade para o primeiro tipo celular, sendo que a maior relação entre CD4+ e CD8+ ocorreu no terceiro dia de idade. Com base nos resultados obtidos neste estudo, notaram-se as diferenças semanais nas populações linfocitárias, o que demonstra a dinâmica dessas células durante o período neonatal.

  13. A importância da auditoria na análise ao pressuposto de continuidade

    OpenAIRE

    Aires, Vanessa Alexandra Campos

    2016-01-01

    Mestrado em Auditoria A análise ao pressuposto de continuidade tem alcançado uma enorme relevância, nos últimos anos, tendo em consideração os inúmeros casos de falência e escândalos que têm ocorrido, como por exemplo, a Enron, a Worldcom e o BES. A insolvência de entidades que foram alvo de auditorias proporcionou inúmeras incertezas, aos utilizadores das demonstrações financeiras, que se questionam quanto à responsabilidade dos auditores relativamente a estas situações. O presente trabal...

  14. A MEMÕRIA DE JESUS E A MANVFESTAÇAO DO PAI NO QUARTO EVANGELHO

    Directory of Open Access Journals (Sweden)

    Johan Konings

    1988-01-01

    Full Text Available Não somos os primeiros a constatar que as cristologias narrativas pouco se inspiram no Quarto Evangelho (QE, tanto na atual teologia latino-americana como na europeia, de E. Schillebeeckx, por exemplo. Entretanto, as cristologias especulativas usam amplamente pelo menos o prólogo de João como ponto de partida para suas especulações. Será que este tratamento faz jus ao QE, ou repousa num preconceito espiritualista com relação ao evangelho que Clemente de Alexandria chamou de "espiritual"?

  15. The Ashkenazi Jews of Curaçao, a trading minority

    Directory of Open Access Journals (Sweden)

    Eva Abraham-Van der Mark

    2000-07-01

    Full Text Available First describes the early Sephardi presence in Curaçao, the arrival of the Ashkenazi in the 20th c., and the relations between these 2 groups. Author goes on to discuss the Ashkenazis' economic success and the exodus of the 1980s. She asks whether the success and the exodus can be attributed to the characteristics of the group itself or whether conditions and developments in Curaçao account for economic fortune and the departure of the Ashkenazi.

  16. Ressalto no canto posteromedial do joelho relacionado ao músculo sartório

    Directory of Open Access Journals (Sweden)

    Marcello Henrique Nogueira-Barbosa

    2011-06-01

    Full Text Available As causas da síndrome de ressalto no joelho relatadas com maior frequência são relacionadas a anormalidades meniscais, ao tendão do músculo bíceps femoral e aos tendões grácil e semitendíneo. Neste trabalho é descrito um caso de ressalto no canto posteromedial relacionado à junção miotendínea do músculo sartório em um paciente do sexo masculino, de 58 anos de idade, com hiperextensão do joelho.

  17. O direito penal na regulaçao da vida e da morte ante a biotecnologia

    OpenAIRE

    Minahim, Maria Auxiliadora

    2005-01-01

    A sociedade pós-industrial tem testemunhado, nas últimas décadas, um desenvolvimento tecnológico e científico que ocorre em velocidade e intensidade superiores ao verificado ao longo de toda a trajetória da humanidade. Este fenômeno produz, como não podia deixa de ser, profundas alterações nas relações humanas. As novas tecnologias têm modificado profundamente o perfil da sociedade, afetando relações, tensionando crenças e valores. Este desenvolvimento tem proposto uma reflexão sobre os limit...

  18. Um modelo de hipertexto para apoio ao ensino mediado pela Web

    OpenAIRE

    Willie Dresler Leiva

    2003-01-01

    Atualmente há uma demanda crescente por aplicações hipermídia baseadas na WWW (World Wide Web), conhecidas como WIS (Web Information Systems). Esse novo tipo de aplicação apresenta requisitos adicionais aos sistemas de software clássicos, o que resulta na necessidade de investigar modelos mais adequados para apoiar o seu desenvolvimento. Em especial, os sistemas para apoio ao EaD (Ensino a Distância) baseados na Web apresentam características e requisitos ainda mais específicos. Os modelos at...

  19. Resistência ao fogo de vigas parcialmente embebidas com betão

    OpenAIRE

    Piloto, P.A.G.; Gavilan, Ana; Mesquita, L.M.R.

    2011-01-01

    Neste artigo serão apresentados resultados dos ensaios de resistência ao fogo de elementos de viga parcialmente embebidos com betão. Os elementos de viga foram ensaiados à flexão, com aplicação de uma carga constante localizada a meio vão, submetida a uma curva de incêndio nominal ISO 834, [1-2]. Foram determinados os tempos de resistência ao fogo, as temperaturas críticas e os modos de colapso para cada grau de utilização.

  20. Quality control of Ling'ao nuclear power station civil construction

    International Nuclear Information System (INIS)

    Lu Buliang; Ye Changyuan

    2001-01-01

    Based on the quality control model adopted during Ling'ao Nuclear Power Station construction, the author briefly introduces quality control process of some main civil construction activities (reinforced concrete, steel liner, steel works and prestressing force) of nuclear power station, and makes some descriptions on non-conformance control of civil works. These quality control processes described come from the concrete practice during civil construction of Ling'ao Nuclear Power Station, and are based on Daya Bay Nuclear Power Station construction experience