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Sample records for antlers

  1. Antler development in reindeer in relation to age and sex

    OpenAIRE

    Amund Høymork; Eigil Reimers

    2002-01-01

    Yearling male and adult female reindeer (Rangifer tarandus tarandus) are similar in size and shape. If sexual clues are hidden, it can be difficult to distinguish between them. Antlers can be a useful aid in classifying yearling males and adult females, depending on whether specific antler characteristics are identifiable for these two groups. We recorded antler characteristics in a domestic reindeer herd (Vågå) and found considerable overlap in antler height, width and circumfere...

  2. Antler development in reindeer in relation to age and sex

    Directory of Open Access Journals (Sweden)

    Amund Høymork

    2002-06-01

    Full Text Available Yearling male and adult female reindeer (Rangifer tarandus tarandus are similar in size and shape. If sexual clues are hidden, it can be difficult to distinguish between them. Antlers can be a useful aid in classifying yearling males and adult females, depending on whether specific antler characteristics are identifiable for these two groups. We recorded antler characteristics in a domestic reindeer herd (Vågå and found considerable overlap in antler height, width and circumference between the different age and sex groups. Total tines and number of tine split-offs are use¬ful for the field biologist when discriminating among adult females, yearling males and 2.5 year-old males. For example, when using the tine split-offs with the suggested classification, 79% of the observed adult females and 76% of the yearling males were classified correctly. The antler height, width and circumference provide other biological dif¬ferences between groups, but are not easy to use to identify free ranging reindeer. This is due to the great overlap in antler size between the groups and measuring difficulties in a field study situation. Male and female calves have very similar antlers, and only the antler width is possible for sex discrimination, giving 67% accuracy of discriminating between these two groups.

  3. The antler finds at Bilzingsleben, excavations 1969-1993

    Directory of Open Access Journals (Sweden)

    Jürgen Vollbrecht

    2000-09-01

    Full Text Available 2820 antler remains from the Lower Palaeolithic site of Bilzingsleben, Thuringia, Germany (excavations 1969-1993 were the subject of detailed investigations. The two major goals were: 1.the consideration of taphonomic aspects 2.the critical evaluation of suggestions about artificial modifications to the antler material A detailed morphological description of the antler material provided the basis for the investigation. A prerequisite was the transfer of provenance data onto an x-y coordinate grid. Taphonomic aspects considered in this work include the relative frequencies of antler elements, estimates regarding the minimum number of individual deer, their age structure and seasonality, and, insofar as the condition of the antlers allowed, the classification of surface preservation, size classes and spatial distribution of the finds. The assemblage of antler finds, the majority of which seems to have come from red deer, is dominated by small fragments, mostly of tines. About one quarter of the finds are larger than 150 mm. Lower beams are more abundant than upper beams (e.g. crowns. Detailed counting, substantiated by systematic reconstruction, shows that in general the antlers are incomplete. After reconstruction of unshed antlers, it was possible to assess the minimum number of heads at 150 animals. Preliminary counting of postcranial and cranial (non antler cervid material points to about 70 cervids. Intentional accumulation of antlers by hominids can only be accepted as the reason for these disproportionate figures if other site formation processes can be ruled out. In fact, the correlation between sediment thickness and maximum antler densities, at least for finds smaller than 120mm, suggests that fluvial accumulation has to be taken into account as a probable element of the site formation history. Further, the mixture of unifacially abraded finds together with finds that exhibit bifacial abrasion points to a succession of changing fluvial

  4. 鹿茸干细胞与鹿茸再生%Antler Stem Cells and Antler Regeneration

    Institute of Scientific and Technical Information of China (English)

    魏明立; 褚文辉; 赵海平; 李春义

    2011-01-01

    鹿茸是唯一能够完全再生的哺乳动物器官,因而成为良好的生物医学研究模型.研究发现,鹿茸的再生是一个基于干细胞的过程,其内在的生长机制和调控模式对再生医学等研究领域具有重大的意义.本文对鹿茸的组织学、形态学及鹿茸干细胞的发现/鉴定进行了总结,进而探讨了鹿茸干细胞内与再生相关的基因.%Antler is the unique mammalian organ that can regenerate completely, so it is a very good biomedical research model. The studies find that antler regeneration is a stem cell-based process and that its inner mechanism and regulatory mode of antler growth is of great significance for us to research the area of regeneration medicine. The article summarizes the histology and morphology of velvet antler, then reviews the discovery and identification of antler stem cells, and last further discusses the related genes of antler regeneration in the antler stem cells.

  5. INTEGRATION IN ANTLER REINDEER BREEDING OF THE ALTAI REPUBLIC

    Directory of Open Access Journals (Sweden)

    Glotko A. V.

    2014-12-01

    Full Text Available In this article the questions of vertical integration which is necessary to bring the company of antler reindeer breeding out of crisis are considered. We have presented a scheme of participant of "supplier – consumer” integral interaction

  6. Digital data sets that describe aquifer characteristics of the Antlers aquifer in southeastern Oklahoma

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set consists of digitized water-level elevation contours for the Antlers aquifer in southeastern Oklahoma. The Early Cretaceous-age Antlers Sandstone is...

  7. Digital data sets that describe aquifer characteristics of the Antlers aquifer in southeastern Oklahoma

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set consists of digitized aquifer boundaries of the Antlers aquifer in southeastern Oklahoma. The Early Cretaceous-age Antlers Sandstone is an important...

  8. Digital data sets that describe aquifer characteristics of the Antlers aquifer in southeastern Oklahoma

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set consists of digitized polygons of constant recharge values for the Antlers aquifer in southeastern Oklahoma. The Early Cretaceous-age Antlers...

  9. Simulating antler growth and energy, nitrogen, calcium and phosphorus metabolism in caribou

    Directory of Open Access Journals (Sweden)

    Ron Moen

    1998-03-01

    Full Text Available We added antler growth and mineral metabolism modules to a previously developed energetics model for ruminants to simulate energy and mineral balance of male and female caribou throughout an annual cycle. Body watet, fat, protein, and ash are monitored on a daily time step, and energy costs associated with reproduction and body mass changes are simulated. In order to simulate antler growth, we had to predict calcium and phosphorus metabolism as it is affected by antler growth, gestation, and lactation. We used data on dietary digestibility, protein, calcium and phosphorus content, and seasonal patterns in body mass to predict the energy, nitrogen, calcium, and phosphorus balances of a "generic" male and female caribou. Antler growth in males increased energy requirements during antler growth by 8 to 16%, depending on the efficiency with which energy was used for antler growth. Female energy requirements for antler growth were proportionately much smaller because of the smaller size of female antlers. Protein requirements for antler growth in both males and females were met by forage intake. Calcium and phosphorus must be resorbed from bone during peak antler growth in males, when > 25 g/day of calcium and > 12 g/day of phosphorus are being deposited in antlers. Females are capable of meeting calcium needs during antler growth without bone resorption, but phosphorus was resorbed from bone during the final stages of antler mineralization. After energy, phosphorus was most likely to limit growth of antlers for both males and females in our simulations. Input parameters can be easily changed to represent caribou from specific geographic regions in which dietary nutrient content or body mass patterns differ from those in our "generic" caribou. The model can be used to quantitatively analyze the evolutionary basis for development of antlers in female caribou, and the relationship between body mass and antler size in the Cervidae.

  10. MicroRNA profiling of antler stem cells in potentiated and dormant states and their potential roles in antler regeneration.

    Science.gov (United States)

    Ba, Hengxing; Wang, Datao; Li, Chunyi

    2016-04-01

    MicroRNAs (miRNAs) can effectively regulate gene expression at the post-transcriptional level and play a critical role in tissue growth, development and regeneration. Our previous studies showed that antler regeneration is a stem cell-based process and antler stem cells reside in the periosteum of a pedicle, the permanent bony protuberance, from which antler regeneration takes place. Antlers are the only mammalian organ that can fully regenerate and hence provide a unique opportunity to identify miRNAs that are involved in organ regeneration. In the present study, we used next generation sequencing technology sequenced miRNAs of the stem cells derived from either the potentiated or the dormant pedicle periosteum. A population of both conserved and 20 deer-specific miRNAs was identified. These conserved miRNAs were derived from 453 homologous hairpin precursors across 88 animal species, and were further grouped into 167 miRNA families. Among them, the miR-296 is embryonic stem cell-specific. The potentiation process resulted in the significant regulation (>±2 Fold, q value stem cell potentiation process. This research has identified miRNAs that are associated either with the dormant or the potentiated antler stem cells and identified some target miRNAs for further research into their role played in mammalian organ regeneration. PMID:26738876

  11. Study on the Changes in Enzyme and Insulin-like Growth Factor-1 Concentrations in Blood Serum and Growth Characteristics of Velvet Antler during the Antler Growth Period in Sika Deer (Cervus nippon).

    Science.gov (United States)

    Park, Jaehyun; Jeon, Byongtae; Kang, Sungki; Oh, Mirae; Kim, Myonghwa; Jang, Seyoung; Park, Pyojam; Kim, Sangwoo; Moon, Sangho

    2015-09-01

    This study was conducted to investigate changes in blood enzyme parameters and to evaluate the relationship between insulin-like growth factor-1 (IGF-1), antler growth and body weight during the antler growth of sika deer (Cervus nippon). Serum enzyme activity and IGF-1 concentrations were measured in blood samples collected from the jugular and femoral veins at regular intervals during the antler growth period. Blood samples were taken in the morning from fasted stags (n = 12) which were healthy and showed no clinical signs of disease. Alfalfa was available ad libitum and concentrates were given at 1% of body weight to all stags. The experimental diet was provided at 9 am with water available at all times. There were no significant differences in alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase during antler growth, but alkaline phosphatase concentrations increased with antler growth progression, and the highest alkaline phosphatase concentration was obtained 55 days after antler casting. Serum IGF-1 concentrations measured from blood samples taken from the jugular vein during antler growth, determined that levels of IGF-1 was associated with body weight and antler growth patterns. Serum IGF-1 concentrations were higher at the antler cutting date than other sampling dates. Antler length increased significantly during antler growth (palkaline phosphatase concentration was related to antler growth and both antler growth and body weight were associated positively with IGF-1 concentrations during antler growth. PMID:26194228

  12. Potential of the Bucharest 3 MV Tandetron™ for IBA studies of deer antler mineralization

    Science.gov (United States)

    Gomez, S.; Garcia, A.; Landete-Castillejos, T.; Gallego, L.; Pantelica, D.; Pantelica, Ana; Preoteasa, E. A.; Scafes, Adela; Straticiuc, M.

    2016-03-01

    Combined PIXE and PIGE analysis was applied at the new Bucharest Tandetron to investigate biomineralization in two calcified tissues, deer antlers and femur bone. By annual loss and fast re-growth, antlers are a valuable model for bone as a dynamical system. Samples characterized by optical microscopy and histology were analyzed for P, Ca, F, Na, Mg, S, Cl, K, Zn, Sr by 3 MeV proton simultaneous PIXE and PIGE, using a hydroxyapatite standard and other reference materials. Good correlation between methods was found for P, and the concentrations were related to biological data. Antlers showed lower mineralization than femur, with the lowest values in the third antler beam. A power function of mineralization vs. "mineral age" of antlers was found. Thus combined PIXE and PIGE of antlers may bring highly relevant insights in biomineralization research.

  13. A PCR-based assay for discriminating Cervus and Rangifer (Cervidae) antlers with mitochondrial DNA polymorphisms.

    Science.gov (United States)

    Kim, Young Hwa; Kim, Eung Soo; Ko, Byong Seob; Oh, Seung-Eun; Ryuk, Jin-Ah; Chae, Seong Wook; Lee, Hye Won; Choi, Go Ya; Seo, Doo Won; Lee, Mi Young

    2012-07-01

    This study describes a method for discriminating Rangifer antlers from true Cervus antlers using agarose gel electrophoresis, capillary electrophoresis, quantitative real-time PCR, and allelic discrimination. Specific primers labeled with fluorescent tags were designed to amplify fragments from the mitochondrial D-loop genes for various Cervus subspecies and Rangifer tarandus differentially. A 466-bp fragment that was observed for both Cervus and Rangifer antlers served as a positive control, while a 270-bp fragment was specifically amplified only from Rangifer antlers. Allelic discrimination was used to differentiate between Cervus and Rangifer antlers, based on the amplification of specific alleles for both types of antlers. These PCR-based assays can be used for forensic and quantitative analyses of Cervus and Rangifer antlers in a single step, without having to obtain any sequence information. In addition, multiple PCR-based assays are more accurate and reproducible than a single assay for species-specific analysis and are especially useful in this study for the identification of original Cervus deer products from fraudulent Rangifer antlers.

  14. Nerve Growth Factor mRNA Expression in the Regenerating Antler Tip of Red Deer (Cervus elaphus)

    Science.gov (United States)

    Li, Chunyi; Stanton, Jo-Ann L.; Robertson, Tracy M.; Suttie, James M.; Sheard, Philip W.; John Harris, A.; Clark, Dawn E.

    2007-01-01

    Deer antlers are the only mammalian organs that can fully regenerate each year. During their growth phase, antlers of red deer extend at a rate of approximately 10 mm/day, a growth rate matched by the antler nerves. It was demonstrated in a previous study that extracts from deer velvet antler can promote neurite outgrowth from neural explants, suggesting a possible role for Nerve Growth Factor (NGF) in antler innervation. Here we showed using the techniques of Northern blot analysis, denervation, immunohistochemistry and in situ hybridization that NGF mRNA was expressed in the regenerating antler, principally in the smooth muscle of the arteries and arterioles of the growing antler tip. Regenerating axons followed the route of the major blood vessels, located at the interface between the dermis and the reserve mesenchyme of the antler. Denervation experiments suggested a causal relationship exists between NGF mRNA expression in arterial smooth muscle and sensory axons in the antler tip. We hypothesize that NGF expressed in the smooth muscle of the arteries and arterioles promotes and maintains antler angiogenesis and this role positions NGF ahead of axons during antler growth. As a result, NGF can serve a second role, attracting sensory axons into the antler, and thus it can provide a guidance cue to define the nerve track. This would explain the phenomenon whereby re-innervation of the regenerating antler follows vascular ingrowth. The annual growth of deer antler presents a unique opportunity to better understand the factors involved in rapid nerve regeneration. PMID:17215957

  15. Digital data sets that describe aquifer characteristics of the Antlers aquifer in southeastern Oklahoma

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set consists of digitized polygons of a constant hydraulic conductivity value for the Antlers aquifer in southeastern Oklahoma. The Early Cretaceous-age...

  16. Mule deer antlers as biomonitors of strontium-90 on the Hanford Site

    International Nuclear Information System (INIS)

    This study evaluated deer antlers as indicators of animal uptake of localized 90Sr contamination on the Hanford Site in south-central Washington. Levels of 90Sr were examined in 38 mule deer (Odocoileus hemionus hemionus) antler samples collected near and distant from previously active nuclear reactor facilities and from a reference site in central Oregon. Results showed that 90Sr concentrations in antlers collected near reactor facilities were significantly higher (P90Sr compared with Hanford. Strontium-90 concentrations in deer antlers collected at the reference locations were higher than Hanford site deer, presumably because the deer inhabited mountain regions during the summer months that received more atmospheric fallout from historic weapons testing

  17. Anti-resorptive effect of pilose antler blood (Cervus nippon Temminck) in ovariectomized rats.

    Science.gov (United States)

    Yang, Jian-Hong; Cao, Yi; Wang, Rui-Lin; Fei, Yu-Rong; Zhang, Hui; Feng, Pu; Liu, Jing

    2010-06-01

    Anti-bone resorption activity of pilose antler blood (Cervus nippon Temminck) were evaluated in ovariectomized Wistar rats. The rats were randomly divided into sham operated group (SHAM), ovariectomized group (OVX) and pilose antler blood treated group. The ovariectomized rats were treated with pilose antler blood orally in 4000 microl/kg daily doses for 10 weeks. Compared with SHAM group, serum 17 beta-estradiol level decreased significantly and osteocalcin level increased significantly in OVX group, indicating successful model of osteoporosis. The experiments showed that the bone mineral density of the lumbar spine and left femur in OVX group decreased remarkably compared to SHAM group but normalized by treatment with pilose antler blood. Additionally, serum levels of insulin-like growth factor-land testosterone were lower obviously in OVX group than those in SHAM group but preserved by pilose antler blood treatment. However, no obvious changes in serum levels of calcium, phosphorus, total alkaline phosphatase and osteoprotegerin were observed among three groups. These results suggested that administration of pilose antler blood was effective in alleviating osteoporosis in ovariectomized rats.

  18. Rapid assessment of quality of deer antler slices by using an electronic nose coupled with chemometric analysis

    OpenAIRE

    Guojie Xu; Caili Liao; Xiaolei Ren; Xue Zhang; Xinyue Zhang; Siqi Liu; Xiaorui Fu; Haozhong Wu; Luqi Huang; Chunsheng Liu; Xueyong Wang

    2014-01-01

    Deer antler is a precious animal-sourced traditional Chinese medicine. We aimed to rapidly assess the quality of deer antler slices by electronic nose so that we can ensure medical safety. In this study, response intensity of the electronic nose was favorably optimized, and samples were well assessed by using an electronic nose based on LDA model. The results obtained herein suggested that electronic nose could be an effective method to rapidly assess the quality of deer antler slices, and co...

  19. A Non-Destructive Method for Distinguishing Reindeer Antler (Rangifer tarandus) from Red Deer Antler (Cervus elaphus) Using X-Ray Micro-Tomography Coupled with SVM Classifiers

    Science.gov (United States)

    Lefebvre, Alexandre; Rochefort, Gael Y.; Santos, Frédéric; Le Denmat, Dominique; Salmon, Benjamin; Pétillon, Jean-Marc

    2016-01-01

    Over the last decade, biomedical 3D-imaging tools have gained widespread use in the analysis of prehistoric bone artefacts. While initial attempts to characterise the major categories used in osseous industry (i.e. bone, antler, and dentine/ivory) have been successful, the taxonomic determination of prehistoric artefacts remains to be investigated. The distinction between reindeer and red deer antler can be challenging, particularly in cases of anthropic and/or taphonomic modifications. In addition to the range of destructive physicochemical identification methods available (mass spectrometry, isotopic ratio, and DNA analysis), X-ray micro-tomography (micro-CT) provides convincing non-destructive 3D images and analyses. This paper presents the experimental protocol (sample scans, image processing, and statistical analysis) we have developed in order to identify modern and archaeological antler collections (from Isturitz, France). This original method is based on bone microstructure analysis combined with advanced statistical support vector machine (SVM) classifiers. A combination of six microarchitecture biomarkers (bone volume fraction, trabecular number, trabecular separation, trabecular thickness, trabecular bone pattern factor, and structure model index) were screened using micro-CT in order to characterise internal alveolar structure. Overall, reindeer alveoli presented a tighter mesh than red deer alveoli, and statistical analysis allowed us to distinguish archaeological antler by species with an accuracy of 96%, regardless of anatomical location on the antler. In conclusion, micro-CT combined with SVM classifiers proves to be a promising additional non-destructive method for antler identification, suitable for archaeological artefacts whose degree of human modification and cultural heritage or scientific value has previously made it impossible (tools, ornaments, etc.). PMID:26901355

  20. Does fluctuating asymmetry of antlers in white-tailed deer (Odocoileus virginianus) follow patterns predicted for sexually selected traits?

    Science.gov (United States)

    Ditchkoff, S.S.; Lochmiller, R.L.; Masters, R.E.; Starry, W.R.; Leslie, David M.

    2001-01-01

    Secondary sexual characters have been hypothesized to signal male quality and should demonstrate a negative relationship between the size of the trait and degree of fluctuating asymmetry because they are costly to produce. We collected morphometric and antler data from 439 white-tailed deer (Odocoileus virginianus) in Oklahoma, USA, in order to determine whether measures of antler asymmetry follow the patterns predicted for sexually selected characters. Relative fluctuating asymmetry was negatively related to antler size for all deer and within age groups up to five and a half years of age. We did not detect an association between asymmetry and antler size among deer that were six and a half years or older. When categorizing deer by antler size, we found that deer with small antlers (???33rd percentile) had greater levels of relative asymmetry than deer with large antlers (???67th percentile). The relative asymmetry of antlers was negatively related to age and was greatest in deer that were one and a half years old. Relative asymmetry was also negatively related to carcass mass, inside spread, skull length and body length. These data suggest that asymmetry in the antlers of white-tailed deer may be a reliable signal of quality and, as such, may be important in maintaining honesty in intrasexual advertisements during the breeding season.

  1. Antlers of Cervus elaphus as biomonitors of {sup 90}Sr in the environment

    Energy Technology Data Exchange (ETDEWEB)

    Baeza, A.; Vallejo, I. [LARUEX, Dpt. Applied Physics, Faculty of Veterinary Sciences, University of Extremadura, Avda, 10003 Caceres (Spain); Guillen, J., E-mail: fguillen@unex.e [LARUEX, Dpt. Applied Physics, Faculty of Veterinary Sciences, University of Extremadura, Avda, 10003 Caceres (Spain); Salas, A.; Corbacho, J.A. [LARUEX, Dpt. Applied Physics, Faculty of Veterinary Sciences, University of Extremadura, Avda, 10003 Caceres (Spain)

    2011-03-15

    Adequate radioprotection of the environment requires the identification of biomonitors sensitive to the variation of its radionuclide content. Due to the chemical similarities between calcium and strontium, calcified tissues of mammals are considered to be good {sup 90}Sr biomonitors. This work considered Cervus elaphus antlers which, being shed annually, can give information about the importance of radiostrontium contamination in an ecosystem in the time period required for the growth of the antler. The samples were collected at various points of W and SW Spain. The mean value of their {sup 90}Sr content was (70 {+-} 43 (S.D.)) Bq/kg d.w., range (16-218) Bq/kg d.w., and the radionuclide was evenly distributed in the different parts of the antler. There was a good correlation between the antlers' {sup 90}Sr content and the {sup 90}Sr deposited in the soil. The antlers' content of {sup 226}Ra (from the natural uranium series) and the contents of some stable elements (Ca, Mg, Sr, and K) were also determined. The values for these stable elements were practically constant in the analyzed samples, and the concentrations measured decreased in the following order: Ca > Mg > K > Sr > {sup 90}Sr > {sup 226}Ra - Research highlights: {yields} There was a good correlation between the antlers' {sup 90}Sr content and the {sup 90}Sr deposited in the soil. {yields} The content of stable elements (Ca, Mg, Sr, K) in the analyzed antlers were practically constant. {yields} The concentrations measured decreased in the following order: Ca > Mg > K > Sr >{sup 90}Sr >{sup 226}Ra.

  2. European roe deer antlers as an environmental archive for fallout (236)U and (239)Pu.

    Science.gov (United States)

    Froehlich, M B; Steier, P; Wallner, G; Fifield, L K

    2016-01-01

    Anthropogenic (236)U and (239)Pu were measured in European roe deer antlers hunted between 1955 and 1977 which covers and extends beyond the period of intensive nuclear weapons testing (1954-1962). The antlers were hunting trophies, and hence the hunting area, the year of shooting and the approximate age of each animal is given. Uranium and plutonium are known to deposit in skeletal tissue. Since antler histology is similar to bone, both elements were expected in antlers. Furthermore, roe deer shed their antlers annually, and hence antlers may provide a time-resolved environmental archive for fallout radionuclides. The radiochemical procedure is based on a Pu separation step by anion exchange (Dowex 1 × 8) and a subsequent U purification by extraction chromatography using UTEVA(®). The samples were measured by Accelerator Mass Spectrometry at the VERA facility (University of Vienna). In addition to the (236)U and (239)Pu concentrations, the (240)Pu/(239)Pu isotopic ratios were determined with a mean value of 0.172 ± 0.023 which is in agreement with the ratio of global fallout (∼0.18). Rather high (236)U/(238)U ratios of the order of 10(-6) were observed. These measured ratios, where the (236)U arises only from global fallout, have implications for the use of the (236)U/(238)U ratio as a fingerprint for nuclear accidents or releases from nuclear facilities. Our investigations have shown the potential to use antlers as a temporally resolved archive for the uptake of actinides from the environment. PMID:26119579

  3. European roe deer antlers as an environmental archive for fallout (236)U and (239)Pu.

    Science.gov (United States)

    Froehlich, M B; Steier, P; Wallner, G; Fifield, L K

    2016-01-01

    Anthropogenic (236)U and (239)Pu were measured in European roe deer antlers hunted between 1955 and 1977 which covers and extends beyond the period of intensive nuclear weapons testing (1954-1962). The antlers were hunting trophies, and hence the hunting area, the year of shooting and the approximate age of each animal is given. Uranium and plutonium are known to deposit in skeletal tissue. Since antler histology is similar to bone, both elements were expected in antlers. Furthermore, roe deer shed their antlers annually, and hence antlers may provide a time-resolved environmental archive for fallout radionuclides. The radiochemical procedure is based on a Pu separation step by anion exchange (Dowex 1 × 8) and a subsequent U purification by extraction chromatography using UTEVA(®). The samples were measured by Accelerator Mass Spectrometry at the VERA facility (University of Vienna). In addition to the (236)U and (239)Pu concentrations, the (240)Pu/(239)Pu isotopic ratios were determined with a mean value of 0.172 ± 0.023 which is in agreement with the ratio of global fallout (∼0.18). Rather high (236)U/(238)U ratios of the order of 10(-6) were observed. These measured ratios, where the (236)U arises only from global fallout, have implications for the use of the (236)U/(238)U ratio as a fingerprint for nuclear accidents or releases from nuclear facilities. Our investigations have shown the potential to use antlers as a temporally resolved archive for the uptake of actinides from the environment.

  4. Chondroitin sulphate extracted from antler cartilage using high hydrostatic pressure and enzymatic hydrolysis

    Directory of Open Access Journals (Sweden)

    Chong-Tai Kim

    2014-12-01

    Full Text Available Chondroitin sulphate (CS, a major glycosaminoglycan, is an essential component of the extracellular matrix in cartilaginous tissues. Wapiti velvet antlers are a rich source of these molecules. The purpose of the present study was to develop an effective isolation procedure of CS from fresh velvet antlers using a combination of high hydrostatic pressure (100 MPa and enzymatic hydrolysis (papain. High CS extractability (95.1 ± 2.5% of total uronic acid was obtained following incubation (4 h at 50 °C with papain at pH 6.0 in 100 MPa compared to low extractability (19 ± 1.1% in ambient pressure (0.1 MPa. Antler CS fractions were isolated by Sephacryl S-300 chromatography and identified by western blot using an anti-CS monoclonal antibody. The antler CS fraction did not aggregate with hyaluronic acid in CL-2B chromatography and possessed DPPH radical scavenging activity at 78.3 ± 1.5%. The results indicated that high hydrostatic pressure and enzymatic hydrolysis procedure may be a useful tool for the isolation of CS from antler cartilaginous tissues.

  5. Iodine distribution in the environment as a limiting factor for roe deer antler development.

    Science.gov (United States)

    Lehoczki, Róbert; Erdélyi, Károly; Sonkoly, Krisztina; Szemethy, László; Csányi, Sándor

    2011-02-01

    The iodine-containing hormones produced by the thyroid gland play a role in the complex neuro-hormonal regulation of antler development. The proper function of the thyroid depends on the adequate iodine supply of the organism, which is directly related to the iodine content of food and drinking water. The purpose of this study was to explore the connection between the iodine content of the water base, which has a strong correlation with the iodine concentration of environmental components available to animals, and the antler weight of roe deer (Capreolus capreolus) shot in Hungarian hunting areas. Using a general linear model, controlling for the collective effects of other environmental factors (deer population density, harvest rate, land use, and soil fertility information), the iodine content of the water base explained 51.4% of the total variance of antler weights. The results suggest that antler weights increase with increasing iodine concentration regardless of other factors; thus, the environmental iodine distribution can be a limiting factor suppressing roe deer performance assessed here as antler weight. Further experimental studies of controlled iodine uptake are needed to define the exact physiological iodine requirements of roe deer bucks.

  6. Determination of Sex Hormones in Antler Velvet by High Performance Liquid Chromatography Tandem Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    LU Chun-mei; WANG Ming-tai; MU Jun; BAI Yu-ping; DU Jian-shi; ZHANG Han-qi; WANG Jian-wei

    2012-01-01

    Eighteen sex hormones in antler velvet were determined by high performance liquid chromatography tandem mass spectrometry.The solid phase extraction was applied to eliminating the matrix effect.The experimental conditions were examined and optimized.Under the optimal conditions,the proposed method provides the good linearities and determination limits(0.2-1.0 μg/kg)of the analytes investigated.The recoveries ranging from 72.3% to 149.5% were obtained for the target analytes at two concentration levels.This method was applied to the determination of eighteen sex hormones in different kinds of antler velvet samples and the obtained results are satisfactory.The results indicate that the proposed method is suitable for the determination of sex hormones in antler velvet samples.

  7. Antler possession by west Greenland female caribou in relation to population characteristics

    Directory of Open Access Journals (Sweden)

    Henning Thing

    1986-06-01

    Full Text Available The frequency of antlerless adult female caribou (Rangifer tarandus groenlandicus was studied in four separate populations in west Greenland. Between the herds antlerlessness varied from 21% to 79%. An inverse relationship between winter range quality and percentage of unantlered cows is demonstrated. Relationship between calf percentage and maternal antler status was studied in one population and antlerless cows showed higher reproductive rate than antlered ones. In another population antlerless cows were almost absent outside the calving area. Calves of antlerless mothers were more susceptible to diseases and had significantly higher summer mortality than other calves, 42% and 27% respectively. The relative importance of factors influencing antler development under various environmental conditons are assessed and a close relationship between antlerlessness, physical condition, lactation, and length of period between calving and midsummer is discussed.

  8. Rapid assessment of quality of deer antler slices by using an electronic nose coupled with chemometric analysis

    Directory of Open Access Journals (Sweden)

    Guojie Xu

    2014-12-01

    Full Text Available Deer antler is a precious animal-sourced traditional Chinese medicine. We aimed to rapidly assess the quality of deer antler slices by electronic nose so that we can ensure medical safety. In this study, response intensity of the electronic nose was favorably optimized, and samples were well assessed by using an electronic nose based on LDA model. The results obtained herein suggested that electronic nose could be an effective method to rapidly assess the quality of deer antler slices, and could also be an important tool for categorization of complex aroma mixtures for the control of quality of drugs or food.

  9. Mechanistic aspects of fracture and R-curve behavior in elk antler bone

    Energy Technology Data Exchange (ETDEWEB)

    Launey, Maximilien E.; Chen, Po-Yu; McKittrick, Joanna; Ritchie, Robert O.

    2009-11-23

    Bone is an adaptative material that is designed for different functional requirements; indeed, bones have a variety of properties depending on their role in the body. To understand the mechanical response of bone requires the elucidation of its structure-function relationships. Here, we examine the fracture toughness of compact bone of elk antler which is an extremely fast growing primary bone designed for a totally different function than human (secondary) bone. We find that antler in the transverse (breaking) orientation is one of the toughest biological materials known. Its resistance to fracture is achieved during crack growth (extrinsically) by a combination of gross crack deflection/twisting and crack bridging via uncracked 'ligaments' in the crack wake, both mechanisms activated by microcracking primarily at lamellar boundaries. We present an assessment of the toughening mechanisms acting in antler as compared to human cortical bone, and identify an enhanced role of inelastic deformation in antler which further contributes to its (intrinsic) toughness.

  10. Urban networks and Arctic outlands: Craft specialists and reindeer antler in Viking towns

    DEFF Research Database (Denmark)

    Ashby, Steven P.; Coutu, Ashley N.; Sindbæk, Søren Michael

    2015-01-01

    This paper presents the results of the use of a minimally destructive biomolecular technique to explore the resource networks behind one of the first specialized urban crafts in early mediaeval northern Europe: the manufacture of composite combs of deer antler. The research incorporates the largest...

  11. Biological effect of velvet antler polypeptides on neural stem cells from embryonic rat brain

    Institute of Scientific and Technical Information of China (English)

    LU Lai-jin; CHEN Lei; MENG Xiao-ting; YANG Fan; ZHANG Zhi-xin; CHEN Dong

    2005-01-01

    Background Velvet antler polypeptides (VAPs), which are derived from the antler velvets, have been reported to maintain survival and promote growth and differentiation of neural cells and, especially the development of neural tissues. This study was designed to explore the influence of VAPs on neural stem cells in vitro derived from embryonic rat brain. Methods Neural stem cells derived from E12-14 rat brain were isolated, cultured, and expanded for 7 days until neural stem cell aggregations and neurospheres were generated. The neurospheres were cultured under the condition of different concentration of VAPs followed by immunocytochemistry to detect the differentiation of neural stem cells. Results VAPs could remarkablely promote differentiation of neural stem cells and most neural stem cells were induced to differentiate towards the direction of neurons under certain concentration of VAPs.Conclusion Neural stem cells can be successfully induced into neurons by VAPs in vitro, which could provide a basis for regeneration of the nervous system.

  12. Antler and Body Size in Black-Tailed Deer: An Analysis of Cohort Effects

    Directory of Open Access Journals (Sweden)

    Johanna C. Thalmann

    2015-01-01

    Full Text Available For long-lived species, environmental factors experienced early in life can have lasting effects persisting into adulthood. Large herbivores can be susceptible to cohort-wide declines in fitness as a result of decreases in forage availability, because of extrinsic factors, including extreme climate or high population densities. To examine effects of cohort-specific extrinsic factors on size of adults, we performed a retrospective analysis on harvest data of 450 male black-tailed deer (Odocoileus hemionus columbianus over 19 years in central California, USA. We determined that population density of females had a more dominant effect than did precipitation on body size of males. Harvest of female deer resulted in increases in the overall size of males, even though a 6-year drought occurred during that treatment period. Body size was most influenced by female population density early in life, while antler size was highly affected by both weather early in life and the year directly before harvest. This study provides insights that improve our understanding of the role of cohort effects in body and antler size by cervids; and, in particular, that reduction in female population density can have a profound effect on the body and antler size of male deer.

  13. Genotyping of velvet antlers for identification of country of origin using mitochondrial DNA and fluorescence melting curve analysis with locked nucleic acid probes.

    Science.gov (United States)

    Ahn, Jeong Jin; Kim, Youngjoo; Hong, Ji Young; Kim, Gi Won; Hwang, Seung Yong

    2016-07-01

    Velvet antlers are used medicinally in Asia and possess various therapeutic effects. Prices are set according to the country of origin, which is unidentifiable to the naked eye, and therefore counterfeiting is prevalent. Additionally, antlers of the Canadian elk, which can generate chronic wasting disease, are prevalently smuggled and distributed in the market. Thus, a method for identifying the country of origin of velvet antlers was developed, using polymorphisms in mitochondrial DNA, fluorescence melting curve analysis and analysis of locked nucleic acids (LNA). This combined method is capable of identifying five genotypes of velvet antlers in a single experiment using two probes. It also has advantages in multiplexing, simplicity and efficiency in genotyping, when compared to real-time PCR or microarrays. The developed method can be used to improve identification rates in the velvet antler market and, by extension, research based on polymorphisms in DNA sequences.

  14. ON THE USAGE OF ANIMAL BONES AND ANTLER DURING THE MIDDLE AGES (9th - 13th century

    Directory of Open Access Journals (Sweden)

    Весна Р. Манојловић-Николић

    2012-12-01

    Full Text Available The processing and production of the bone and antler objects could be traced deep into the past. The most beautiful specimens were known as the objects of Paleolithic art, but the tools were also made of these materials. The peak in bone and antler processing was reached during Roman period. During the Middle Ages, mostly utilitarian items were produced especially in the period from the ninth to the thirteenth century.From the chemistry view, the bones were composed of organic and inorganic substances. The bones had tenacity thanks to minerals, but the organic substances gave them flexibility. The most important organic and inorganic components of the bone tissue weree not chemically bounded, so it was possible to make changes in their ratio by physical or chemical acting. The data about technology and processing of the bone and antler objects that referred to the craft in this period could not be found in the written sources. Reconstruction of the processing could be made on the basis of ethnological data and partly modern industry.The bone and antler objects are usually classified in two main groups: tools, essentials in everyday life, and decorative objects made by more skillful craftsmen. The further classifying is done on the basis of their shape and function. It is possible to mark off the items: tools, decorative and toilette requisites, game objects, musical instruments and the parts of the horse equipment. This is the most usual classification of the bone and antler objects in literature. Majority of bone and antler objects, modest for processing but with emphasized functionality, were used during everyday jobs such as skin processing, footwear and clothing making, sewing, fisherman nets making, pottery vessels decorating etc. All the necessary items were made in this way in a settlement. Their functionality, simplicity in making and readily available materials were the reflection of that processing. The found bone and antler objects

  15. Structure and mineralisation density of antler and pedicle bone in red deer (Cervus elaphus L.) exposed to different levels of environmental fluoride: a quantitative backscattered electron imaging study

    Science.gov (United States)

    KIERDORF, UWE; KIERDORF, HORST; BOYDE, ALAN

    2000-01-01

    The structure and relative degree of mineralisation of antler and pedicle bone of yearling red deer stags exposed either to low or high levels of environmental fluoride were determined by digital quantitative backscattered electron (BSE) imaging. Bone fluoride content (BFC) in antlers (845±86 mg F−/kg ash, arithmetic mean± S.E.M.) and pedicles (1448±154 mg F−/kg ash) of deer from a highly fluoride polluted area in North Bohemia (Czech Republic) were significantly higher (P fluoride induced disturbance of bone mineralisation. The rapid growth of antlers leads both to a high mineral demand and a high rate of fluoride uptake during antlerogenesis. This, and the limited lifespan of antlers, which does not allow for a compensation of a delay in the onset or progression of the mineralisation process, renders antler bone particularly susceptible to fluoride. Antlers are therefore considered a useful model for studying fluoride effects on bone formation. Furthermore, analysis of cast antlers enables a noninvasive monitoring of environmental pollution by fluorides. PMID:10697290

  16. MHC, parasites and antler development in red deer: no support for the Hamilton & Zuk hypothesis.

    Science.gov (United States)

    Buczek, M; Okarma, H; Demiaszkiewicz, A W; Radwan, J

    2016-03-01

    The Hamilton-Zuk hypothesis proposes that the genetic benefits of preferences for elaborated secondary sexual traits have their origins in the arms race between hosts and parasites, which maintains genetic variance in parasite resistance. Infection, in turn, can be reflected in the expression of costly sexual ornaments. However, the link between immune genes, infection and the expression of secondary sexual traits has rarely been investigated. Here, we explored whether the presence and identity of functional variants (supertypes) of the highly polymorphic major histocompatibility complex (MHC), which is responsible for the recognition of parasites, predict the load of lung and gut parasites and antler development in the red deer (Cervus elaphus). While we found MHC supertypes to be associated with infection by a number of parasite species, including debilitating lung nematodes, we did not find support for the Hamilton-Zuk hypothesis. On the contrary, we found that lung nematode load was positively associated with antler development. We also found that the supertypes that were associated with resistance to certain parasites at the same time cause susceptibility to others. Such trade-offs may undermine the potential genetic benefits of mate choice for resistant partners. PMID:26687843

  17. [Molecular identification of hairy antler by analysis of high resolution melting].

    Science.gov (United States)

    Chen, Kang; Jiang, Chao; Yuan, Yuan; Huang, Lu-qi; Jin, Yan

    2015-02-01

    High resolution melting (HRM) , an important technology for genotyping and mutation scanning, has broad prospects in the authenticity of traditional Chinese medicine. This paper selected universal CO I primers and used HRM to establish a new method for authenticity of Hairy Antler. PCR was conducted at the annealing temperature of 60 °C and 45 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further optimized. The results showed that the Tm values of Cervus nippon were (81.96 ± 0.07), (84.51 ± 0.03) °C and Cervus elaphus was(82.58 ± 0.13), (85.95 ± 0.05) °C with 10-100 mg · L(-1) DNA template, 0.2 µLmol · L(-1) primer, 2.0 mmol · L(-1) Mg2+. This method can authenticate of hairy antler and is simple, fast, high-throughput, visualization. PMID:26137679

  18. Effects of p21 Gene Down-Regulation through RNAi on Antler Stem Cells In Vitro.

    Directory of Open Access Journals (Sweden)

    Qianqian Guo

    Full Text Available Cell cycle is an integral part of cell proliferation, and consists mainly of four phases, G1, S, G2 and M. The p21 protein, a cyclin dependent kinase inhibitor, plays a key role in regulating cell cyclevia G1 phase control. Cells capable of epimorphic regeneration have G2/M accumulation as their distinctive feature, whilst the majority of somatic cells rest at G1 phase. To investigate the role played byp21 in antler regeneration, we studied the cell cycle distribution of antler stem cells (ASCs, via down-regulation of p21 in vitro using RNAi. The results showed that ASCs had high levels of p21 mRNA expression and rested at G1 phase, which was comparable to the control somatic cells. Down-regulation of p21 did not result in ASC cell cycle re-distribution toward G2/M accumulation, but DNA damage and apoptosis of the ASCs significantly increased and the process of cell aging was slowed. These findings suggest that the ASCs may have evolved to use an alternative, p21-independent cell cycle regulation mechanism. Also a unique p21-dependent inhibitory effect may control DNA damage as a protective mechanism to ensure the fast proliferating ASCs do not become dysplastic/cancerous. Understanding of the mechanism underlying the role played by p21 in the ASCs could give insight into a mammalian system where epimorphic regeneration is initiated whilst the genome stability is effectively maintained.

  19. Effects of p21 Gene Down-Regulation through RNAi on Antler Stem Cells In Vitro.

    Science.gov (United States)

    Guo, Qianqian; Wang, Datao; Liu, Zhen; Li, Chunyi

    2015-01-01

    Cell cycle is an integral part of cell proliferation, and consists mainly of four phases, G1, S, G2 and M. The p21 protein, a cyclin dependent kinase inhibitor, plays a key role in regulating cell cyclevia G1 phase control. Cells capable of epimorphic regeneration have G2/M accumulation as their distinctive feature, whilst the majority of somatic cells rest at G1 phase. To investigate the role played byp21 in antler regeneration, we studied the cell cycle distribution of antler stem cells (ASCs), via down-regulation of p21 in vitro using RNAi. The results showed that ASCs had high levels of p21 mRNA expression and rested at G1 phase, which was comparable to the control somatic cells. Down-regulation of p21 did not result in ASC cell cycle re-distribution toward G2/M accumulation, but DNA damage and apoptosis of the ASCs significantly increased and the process of cell aging was slowed. These findings suggest that the ASCs may have evolved to use an alternative, p21-independent cell cycle regulation mechanism. Also a unique p21-dependent inhibitory effect may control DNA damage as a protective mechanism to ensure the fast proliferating ASCs do not become dysplastic/cancerous. Understanding of the mechanism underlying the role played by p21 in the ASCs could give insight into a mammalian system where epimorphic regeneration is initiated whilst the genome stability is effectively maintained.

  20. Historical biomonitoring of environmental pollution with lead and Fluorides inthe Šalek valley with the aid of roe deer antlers

    OpenAIRE

    Pokorny, Boštjan

    2006-01-01

    Contents of lead and fluorides were determined in the historical set of 129 roe deer antlers, shot in the period 1961-2004 in the vicinity of the Šoštanj Thermal Power Plant (ŠTPP), the Šalek Valley, Slovenia. The highest levels of both pollutants were determined in antlers from the sixties/eighties (Pb: ? = 4.21 } 2.57 mg/kg, max = 7.28 mg/kg; F-: ? = 1453 } 113 mg/kg, max = 2590 mg/kg), while the lowest levels were found in the most recent antlers (2000-2004 period: Pb: ? = 0.58 } 0.11 mg/k...

  1. Anti-inflammatory effects of enzymatic hydrolysates of velvet antler in RAW 264.7 cells in vitro and zebrafish model

    OpenAIRE

    Lee, Seung-Hong; Yang, Hye-Won; Ding, Yuling; Wang, Yanmei; Jeon, You-Jin; Moon, Sang-Ho; Jeon, Byong-Tae; Sung, Si-Heung

    2015-01-01

    Enzymatic hydrolysis has been successfully used for the extraction of numerous biologically active components from a wide variety of natural sources. In the present study, velvet antler was subjected to the extraction process using Alcalase protease. We analyzed bioactive components, such as uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs), and sialic acid, present in the velvet antler Alcalase hydrolysate (VAAH) and assessed their anti-inflammatory effects in zebrafish as well as in ...

  2. Bone regeneration strategy inspired by the study of calcification behavior in deer antler.

    Science.gov (United States)

    Shi, Haishan; Yu, Tao; Li, Zhaoyang; Lu, William; Zhang, Ming; Ye, Jiandong

    2015-12-01

    Bone regeneration has attracted much attention from various researchers and inspired numerous strategies for bone formation. In this study, rapid calcification of deer antlers was studied to unravel bone biology by investigating mineral composition, morphology and microstructure. Calcification model was hypothesized and preliminarily established by in vitro experiments. In our model, mineral deposition and phase conversions in the gel matrix were mimicked. Results revealed that mineral metabolism including deposition and phase conversion plays key roles in calcification in vivo, which inspired the bone regeneration strategy with three main components, i.e. enhanced mineral nucleation, mineral ions sources and crystals habits. Rapid mineral metabolism of implant apatite biomaterials was supposed as the critical aspect of bone regeneration. This study will provide a relatively ideal model for peer bone regeneration studies.

  3. Location analysis and strontium-90 concentrations in deer antlers on the Hanford Site

    International Nuclear Information System (INIS)

    The primary objective of this study was to examine the levels of strontium-90 (90Sr) in deer antlers collected from near previously active reactor sites and distant from the reactor sites along that portion of the Columbia River which borders the Hanford Site. A second objective was to analyze the movements and home-ranges of mule deer residing within these areas and determine to what extent this information contributes to the observed 90Sr concentrations. 90Sr is a long-lived radionuclide (29.1 year half life) produced by fission in irradiated fuel in plutonium production reactors on the Hanford Site. It is also a major component of atmospheric fallout from weapons testing. Concentrations of radionuclides found in the developed environment onsite do not pose a health concern to humans or various wildlife routinely monitored. However, elevated levels of radionuclides in found biota may indicate routes of exposure requiring attention

  4. Effects of velvet antler polypeptide on sexual behavior and testosterone synthesis in aging male mice

    Science.gov (United States)

    Zang, Zhi-Jun; Tang, Hong-Feng; Tuo, Ying; Xing, Wei-Jie; Ji, Su-Yun; Gao, Yong; Deng, Chun-Hua

    2016-01-01

    Twenty-four-month-old male C57BL/6 mice with low serum testosterone levels were used as a late-onset hypogonadism (LOH) animal model for examining the effects of velvet antler polypeptide (VAP) on sexual function and testosterone synthesis. These mice received VAP for 5 consecutive weeks by daily gavage at doses of 100, 200, or 300 mg kg−1 body weight per day (n = 10 mice per dose). Control animals (n = 10) received the same weight-based volume of vehicle. Sexual behavior and testosterone levels in serum and interstitial tissue of testis were measured after the last administration of VAP. Furthermore, to investigate the mechanisms of how VAP affects sexual behavior and testosterone synthesis in vivo, the expression of steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), and 3β-hydroxysteroid dehydrogenase (3β-HSD) in Leydig cells was also measured by immunofluorescence staining and quantitative real-time PCR. As a result, VAP produced a significant improvement in the sexual function of these aging male mice. Serum testosterone level and intratesticular testosterone (ITT) concentration also increased in the VAP-treated groups. The expression of StAR, P450scc, and 3β-HSD was also found to be enhanced in the VAP-treated groups compared with the control group. Our results suggested that VAP was effective in improving sexual function in aging male mice. The effect of velvet antler on sexual function was due to the increased expression of several rate-limiting enzymes of testosterone synthesis (StAR, P450scc, and 3β-HSD) and the following promotion of testosterone synthesis in vivo. PMID:26608944

  5. Full length cDNA cloning and expression analysis of annexinA2 gene from deer antler tissue

    Institute of Scientific and Technical Information of China (English)

    Li Hao; Xianghong Xiao; Heping Li

    2014-01-01

    ANXA2(AnnexinA2), a calcium-dependent phospholipid bind-ing protein, is involved in various Ca2+-related biological activities. In the present study, full-length cDNA of ANXA2 was isolated from the velvet antler tip tissue of sika deer (Cervus nippon hortulorum);the amino acid sequence and gene expression was analyzed by using bioinformatics and real-time reverse transcriptase polymerase chain reaction (RT-PCR) techniques. Nucleotide sequence analysis reveals that the full-length cDNA of the ANXA2 gene was 1372 bp, of which 1020 bp was in the open-reading frame (ORF) encoding 339 amino acids; its relative mo-lecular weight was 38.3 kDa; and isoelectric point was 6.72. Sequence analysis indicates that the protein includes four conserved tan-dem-duplication ANX domains. The gene-accession nucleotide sequence number in GenBank is JX315571. Expression analysis by RT-PCR re-veals that ANXA2 gene expression has a significant positive correlation with the antler-tissue mineralization process, indicating that this gene may play an important role in the regulation of antler-tissue mineraliza-tion.

  6. [Simultaneous determination of eleven sex hormones in antler velvet health products by gas chromatography-tandem mass spectrometry].

    Science.gov (United States)

    Lu, Chunmei; Wang, Mingtai; Mu, Jun; Lu, Lijun; Zhou, Xiao

    2011-06-01

    A method for the simultaneous determination of 11 sex hormones in antler velvet health products by gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed. The sex hormones in antler velvet were enriched and purified by solid phase extraction and derivatized with heptafluorobutyric acid anhydride (HFBA). A DB-5 column (30 m x 0.25 mm, 0.25 microm) with nonlinear gradient program was used in GC separation. The sex hormones were determined in the multiple reaction monitoring mode. The method realized the complete separation of 11 sex hormones. The limits of detection of this method were from 1.0 to 5.0 microg/kg for the 11 sex hormones. The correlation coefficients were between 0.991 6 and 0.999 9. The recoveries were in the range of 67.4% - 99.1% with relative standard deviations (RSDs) of 2.6% - 13%. This method is accurate and reliable for the determination of the sex hormones in antler velvet health products.

  7. 3D-ANTLERS: Virtual Reconstruction and Three-Dimensional Measurement

    Science.gov (United States)

    Barba, S.; Fiorillo, F.; De Feo, E.

    2013-02-01

    The main objective of this paper is to establish a procedural method for measuring and cataloguing antlers through the use of laser scanner and of a 3D reconstruction of complex modeling. The deer's antlers have been used as a test and subjected to capture and measurement. For this purpose multiple data sources techniques have been studied and compared, (also considering low-cost sensors) estimating the accuracy and its errors in order to demonstrate the validity of the process. A further development is the comparison of results with applications of digital photogrammetry, considering also cloud computing software. The study has began with an introduction to sensors, addressing the underlying characteristics of the technology available, the scope and the limits of these applications. We have focused particularly on the "structured light", as the acquisition will be completed through three-dimensional scanners: DAVID and the ARTEC MH. The first is a low-cost sensor, a basic webcam and a linear laser pointer, red coloured, that leads to acquisition of three-dimensional strips. The other one is a hand scanner; even in this case we will explain how to represent a 3D model, with a pipeline that provides data export from the "proprietary" to a "reverse engineering" software. Typically, these are the common steps to the two approaches that have been performed in WRAP format: point sampling, manual and global registration, repair normals, surface editing and texture projection. In fact, after a first and common data processing was done with the use of a software supplied with the equipment, the proto-models thus obtained were treated in Geomagic Studio, which was also chosen to allow the homogenization and standardization of data in order to make a more objective comparison. It is commonplace to observe that the editing of the digital mock-up obtained with the DAVID - which had not yet been upgraded to the 3.5 release at the time of this study - is substantially different

  8. Morphogenetic Mechanisms in the Cyclic Regeneration of Hair Follicles and Deer Antlers from Stem Cells

    Directory of Open Access Journals (Sweden)

    Chunyi Li

    2013-01-01

    Full Text Available We have made comparisons between hair follicles (HFs and antler units (AUs—two seemingly unrelated mammalian organs. HFs are tiny and concealed within skin, whereas AUs are gigantic and grown externally for visual display. However, these two organs share some striking similarities. Both consist of permanent and cyclic/temporary components and undergo stem-cell-based organogenesis and cyclic regeneration. Stem cells of both organs reside in the permanent part and the growth centres are located in the temporary part of each respective organ. Organogenesis and regeneration of both organs depend on epithelial-mesenchymal interactions. Establishment of these interactions requires stem cells and reactive/niche cells (dermal papilla cells for HFs and epidermal cells for AUs to be juxtaposed, which is achieved through destruction of the cyclic part to bring the reactive cells into close proximity to the respective stem cell niche. Developments of HFs and AUs are regulated by similar endocrine (particularly testosterone and paracrine (particularly IGF1 factors. Interestingly, these two organs come to interplay during antlerogenesis. In conclusion, we believe that investigators from the fields of both HF and AU biology could greatly benefit from a comprehensive comparison between these two organs.

  9. HEPATOPROTECTIVE ACTION OF POLAR LIPIDS OF MARAL ANTLERS AND PEAT IN EXPERIMENTAL LIVER DAMAGE CAUSED BY ISONIAZIDE AND PARACETAMOL

    Directory of Open Access Journals (Sweden)

    A. I. Yatsenkov

    2013-01-01

    Full Text Available In experimental liver pathology caused by isoniazide or paracetamol administration to albino rats lipids derived from maral antlers and peat decreased the blood activity of aminotransferases, γ-glutamyltranspeptidase, acid and alkaline phosphatases, phospholipase А, content of common bilirubin, activated the detoxication of biliribin, ammonium and phenols, inhibited the liver formation of dienic conjugates, Schiff’s bases, malonic dialdehyde, improve the reduced glutathione function. Maral anthlers and peat lipids in effective doses 30 and 60 mg/kg had the more pronounced hepatoprotective and antioxidant action than lipids in dose 10 mg/kg and essentiale forte N.

  10. Treatment for intractable anemia with the traditional Chinese medicines Hominis Placenta and Cervi Cornus Colla (deer antler glue

    Directory of Open Access Journals (Sweden)

    Yasuyo Hijikata

    2009-05-01

    Full Text Available Yasuyo Hijikata1, Takashi Kano2, Lu Xi31Toyodo Hijikata Clinic, Osaka, Japan; 2Kano Clinic, Osaka city, Osaka, Japan; 3Traditional Chinese Medicine Institute, Si-chuan Province, ChinaObjective: Intractable anemia, such as aplastic anemia or that presumably associated with chronic herpes virus infections, sometimes require bone marrow transplant. We investigated the use of traditional Chinese medicine (TCM for the treatment of intractable anemia. Method: Placenta Hominis (PH, steam boiled and roasted, and Cervi Cornus Colla (deer antler glue has been used in China for hundreds of years to treat anemia. After consent was obtained, we prescribed these two materials for a 74-year-old female with aplastic anemia and a 26-year-old male with presumably a virus-induced anemia. Concomitant conventional therapy was continued in both patients as prescribed by their respective attending physicians. Conclusion: Conventional therapy with steroid hormones, immunosuppressive drugs, platelet and erythrocyte transfusions were not effective in these patients. In addition, both patients suffered from serious side effects. In two patients, ingestion of Placenta Hominis and Cervi Cornus Colla with TCM prescriptions increased the platelet and enhanced the hemoglobin concentration in several months of therapy accompanied by a dramatic improvement in quality of life. The addition to conventional therapy of PH and Cervi Cornus Colla, the latter of which is very easy to obtain, may be one of the potentially advantageous choices in case of otherwise intractable anemia.Keywords: placenta, antler glue, Cervi Cornus Colla, anemia, aplastic anemia

  11. 浅谈麇鹿茸的药用价值%Pharmacology of Velvet Antlers of P è re David Deer

    Institute of Scientific and Technical Information of China (English)

    邱胜荣; 李坤; 张林源

    2011-01-01

    P è re david deer is one native deer species in China, belongs to the category of the 1st class protected animals, which had big contribution to our nation on ancient time. Its antlers, fats, fleshes and bloods etc. Have medical value. Velvet antler contains some kinds of effective chemical components. It has long been esteemed as a rarity Chinese medicine, It is record in Ancient Chinese medical books. Velvet antlers contain a lot of vitamin, amino acid, minerals. In clinical practice, there is strong evidence about the effects of velvet antler on gynecological diseases.%麋鹿是我国特有鹿科动物,在古代曾广泛分布于长江流域大部分地区,其茸、角、脂、血、肉等均具有药用价值.麋鹿茸是珍惜名贵中药材,在古代医学著作中多有记载,后由于麋鹿在野外灭绝而逐渐失传.麋鹿茸中含有丰富的矿物质、维生素、氨基酸和雌二醇,临床实验证明,麋鹿茸在治疗妇科疾病上有较好疗效.

  12. Historical biomonitoring of fluoride pollution by determining fluoride contents in roe deer (Capreolus capreolus L.) antlers and mandibles in the vicinity of the largest Slovene thermal power plant.

    Science.gov (United States)

    Jelenko, Ida; Pokorny, Boštjan

    2010-12-15

    Roe deer antlers/mandibles are a useful tool for determination of ambient fluoride pollution. Antlers have a well-defined annual cycle of growth, therefore they represent a natural standardisation of samples during winter months. On the contrary, mandibles accumulate fluorides during the whole life of an organism, thus they reflect aggregated effect of fluoride pollution trough the life-span of an organism. Both tissues are easily available; mandibles are often systematically collected with the purpose of cognitive management and control, and antlers could be gathered from private well-dated hunters' collections. Considering these benefits, fluoride contents were measured in 141 antlers (period 1960-2007) and 220 mandibles (period 1997-2009) of roe deer, shot in the vicinity of the largest Slovene Thermal Power Plant of Šoštanj (ŠTPP) as one of the major sources of fluorides in Slovenia. Fluoride contents in antlers significantly differed among age categories, and ranged from 110 to 1210 mg/kg in yearlings, 130 to 2340 mg/kg in young adults, and 250 to 2590 mg/kg in older adults, respectively. Fluoride levels in mandibles were also significantly different among age categories, and ranged from 30.0 to 227 mg/kg in fawns, 33.8 to 383 mg/kg in yearlings, and 61.5 to 1020 mg/kg in adults, respectively. Comparison of these results with previously reported fluoride contents in antlers and mandibles of roe deer from different areas of Europe revealed that the study area has never been extensively contaminated with fluorides. Moreover, trends of fluoride contents in both tissues confirmed a significant decrease of fluoride pollution in the area after the years 1995 and 2000, when flue-gas cleaning devices were constructed on the ŠTPP. Indeed, highly positive correlations between annual emissions from the ŠTPP and mean annual fluoride contents in antlers/mandibles confirmed that both tissues may be a useful tool for assessing temporal trends in ambient fluoride

  13. 鹿茸线粒体DNA的指纹鉴定研究%Identification of Velvet Antler by Mitochondrial DNA Fingerprint

    Institute of Scientific and Technical Information of China (English)

    谷玉娟; 张丽华; 傅桂莲

    2013-01-01

    OBJECTIVE To compare specific primer method and random amplified polymorphic DNA ( RAPD) method for identification of Velvet Antler, thus to choose a simpler method. METHODS DNA samples were extracted from sika deer antler, wild deer antler, reideer pilose antler and commercially available Velvet Antler, and then purified and amplified with specific primer amplification and RAPD amplification. RESULTS The sika deer antler, wild deer antler, reideer pilose antler and some of the commercially available Velvet Antler all showed 313 bp segment in the agarose gel with different brightness while the other commercial products did not show this segment. RAPD not only effectively showed positive and negative DNA amplification results, but also showed the difference in PCR products of sika deer antlerwild deer antler with different strip numbers and strip brightness. CONCLUSION RAPD method is more accurate and rapid and it has wide application prospect.%目的 通过鹿茸特异性引物鉴别和随机扩增多态DNA(RAPD)鉴别的比较,选择一种更简便的方法用于鉴别鹿茸.方法 采用盐析法从梅花鹿茸、马鹿茸、驯鹿茸、市售鹿茸等样品中抽提线粒体DNA,并应用试剂盒进行纯化.进行特异性引物扩增和序列测定,同时进行随机扩增多态DNA扩增.结果 梅花鹿茸,马鹿茸,驯鹿茸以及部分市售鹿茸经特异性引物扩增后均可在琼脂糖凝胶中显示313 bp片段,只是条带亮度不同.而其余市售鹿茸无扩增条带;随机扩增多态DNA不仅有效显示阳性与阴性的DNA扩增结果,而且对梅花鹿茸,马鹿茸,驯鹿茸的聚合酶链反应产物的差异,以不同的条带数目和条带亮度得以验证.结论 随机扩增多态DNA鉴别鹿茸真伪的方法更准确快捷,通过随机扩增多态DNA扩增后主条带与梅花鹿茸或马鹿茸扩增的条带大小和亮度一致,则为正品;如不一致,则为《中国药典》规定外的鹿茸或其他混淆品.这种方法

  14. Effects of aging and dietary antler supplementation on the calcium-regulating hormones and bone status in ovariectomized SAMP8 mice.

    Science.gov (United States)

    Chen, Chun-Chi; Liu, Mei-Hui; Wang, Ming-Fu; Chen, Cheng-Chin

    2007-12-31

    This study was conducted to investigate the effects of aging and long-term dietary antler supplementation on the calcium-regulating hormones and bone status in ovariectomized (Ovx) SAMP8 mice. The female SAMP8 mice were divided into four groups (in each group n = 6), Ovx or sham operated at the age of 2 months, and fed with 0.2% antler containing diet or control diet from the age of 2.5 months. The samples were collected at the age of 3, 6, 9, 12, and 15 months, respectively, for physicochemical analyses, biochemical analyses, and the determination of hormones by radioimmunoassay. The results showed that plasma calcium (Ca) concentrations were maintained in a narrow range in all groups throughout the whole experimental period. With aging and/or ovariectomy, plasma parathyroid hormone (PTH) and 1,25-dihydroxycholecalciferol (1,25-(OH)2-D3) levels increased, and plasma phosphorus (P) and calcitonin (CT) levels decreased, and the femoral bone densities and Ca contents increased during the earlier stage, and then decreased gradually in all groups. Plasma PTH and 1,25-(OH)2-D3 levels in the Ovx mice were significantly higher than those in the intact mice, and plasma P concentrations, plasma CT levels, femoral bone densities, and femoral Ca contents in the Ovx mice were significantly lower than those in the intact mice. In addition, the decreases of plasma P levels, plasma CT levels, femoral bone densities, and femoral Ca contents, and the increases of plasma PTH levels were moderated by antler administration in both Ovx and intact mice. However, there was no effect of the dietary antler supplementation on the plasma 1,25-(OH)2-D3 levels in the female mice. It is concluded that prolonged dietary antler supplementation has important positive effects on bone loss with age and/ or ovarian function deficiency.

  15. Study on Deer Antler Growth Behavior of Captive Manchurian Wapiti%圈养东北马鹿鹿茸生长研究

    Institute of Scientific and Technical Information of China (English)

    郝京民; 何绍纯; 由玉岩

    2012-01-01

    2012年3月8日至5月22日,对北京动物园圈养的一只雄性东北马鹿鹿茸生长进行了研究。每间隔8~12d对鹿茸进行拍照,按比例测算鹿茸的相对长度,在锯鹿茸当日测定鹿茸重量。结果表明:圈养条件下,东北马鹿鹿茸主干生长随时间变化呈"S"型生长曲线,即呈缓慢、快速和减速生长3个阶段。第1阶段为第0~29天,平均生长速度为0.17cm/d;第2阶段为第30~56天,平均生长速度为1.30cm/d;第3阶段为第57天后,鹿茸平均生长速度为0.94cm/d。鹿茸3个阶段生长速率差异显著(P〈0.01)。第72天鹿茸锯下时重量4.0kg,平均增重0.05kg/d;右角主干长度54.5cm,平均生长速度0.76cm/d;左角主干长度56.5cm,平均生长速度0.78cm/d。%From March 8th,2012 to May 22nd,2012,the deer antler growth behavior of a male Cervus elaphus xanthopygus was studied in Beijing Zoo using focal animal sampling method. The sampling intervals were 8-12 days. Different stages of deer antler were taken pictures, the data of the deer antler relative lengths were calculated ,and the deer antler was weighted when amputated. The results showed that the growth of the deer antler main branch of the captive manchurian wapiti was in a ' S' shaped growth curve, which composed by three stages, with time going showed slow, fast and deceleration respectively. The first stage was at 0-29 days of the deer antler trunk growth ,with an average growth rate of 0.17 cm/d. The second stage was at 30-56 days, with an average growth rate of 1.30 cm/d. The third stage was after 57 days,with average growth rate of 0.94 cm/d. The growth rate in three stages were significantly different (P〈0.01).The deer antler was 4.0 kg when sawed after 72 days. The weight increased by 0.05kg/d.The right main branch of the deer antler was 54.5 cm in length, and the average growth rate was 0.76 cm/d. The left main branch of the deer antler was 56.5 cm in length, and the average growth rate was 0.78 cm/d.

  16. First Evaluation of the Biologically Active Substances and Antioxidant Potential of Regrowth Velvet Antler by means of Multiple Biochemical Assays

    Directory of Open Access Journals (Sweden)

    Yujiao Tang

    2015-01-01

    Full Text Available We investigated the biologically active substances contained in RVA (regrowth velvet antler by comparing the composition of biologically active substances and antioxidant potential of different antler segments. RVA was subjected to extraction using DW (distilled water. RVA was divided into 3 segments: T-RVA (top RVA, M-RVA (middle RVA, and B-RVA (base RVA. The T-RVA section possessed the greatest amounts of uronic acid (36.251 mg/g, sulfated GAGs (sulfated glycosaminoglycans (555.76 mg/g, sialic acid (111.276 mg/g, uridine (0.957 mg/g, uracil (1.084 mg/g, and hypoxanthine (1.2631 mg/g. In addition, the T-RVA section possessed the strongest antioxidant capacity as determined by DPPH, H2O2 (hydrogen peroxide, hydroxyl, and ABTS (2,2′-azinobis-3-ethylbenzthiazoline-6-sulphonate radical scavenging activity as well as FRAP (ferric reducing antioxidant power and ORAC (oxygen radical absorbance capacity. The values of those were 53.44, 23.09, 34.12, 60.31, and 35.81 TE/μM at 1 mg/mL and 113.57 TE/μM at 20 μg/mL. These results indicate that the T-RVA section possesses the greatest amount of biologically active substances and highest antioxidant potential. This is the first report on the biologically active substances and antioxidant potential of RVA.

  17. Velvet antler peptide prevents pressure overload-induced cardiac fibrosis via transforming growth factor (TGF)-β1 pathway inhibition.

    Science.gov (United States)

    Zhao, Lihong; Mi, Yang; Guan, Hongya; Xu, Yan; Mei, Yingwu

    2016-07-15

    Velvet antlers (VAs) are commonly used in traditional Chinese medicine and invigorant and contain many functional components for health promotion. The velvet antler peptide sVAP32 is one of active components in VAs; based on structural study, the sVAP32 interacts with TGF-β1 receptors and disrupts the TGF-β1 pathway. We hypothesized that sVAP32 prevents cardiac fibrosis from pressure overload by blocking TGF-β1 signaling. Sprague-Dawley rats underwent transverse aortic constriction (TAC) or a sham operation. After one month, rats received either sVAP32 (15mg/kg/day) or vehicle for an additional one month. TAC surgery induced significant cardiac dysfunction, fibroblast activation and fibrosis; these effects were improved by treatment with sVAP32. In the heart tissue, TAC remarkably increased the expression of TGF-β1 and connective tissue growth factor (CTGF), reactive oxygen species levels, and the phosphorylation levels of Smad2/3 and extracellular signal-regulated kinases 1/2 (ERK1/2). SVAP32 inhibited the increases in reactive oxygen species levels, CTGF expression and the phosphorylation of Smad2/3 and ERK1/2, but not TGF-β1 expression. In cultured cardiac fibroblasts, angiotensin II (Ang II) had similar effects compared to TAC surgery, such as increases in α-SMA-positive cardiac fibroblasts and collagen synthesis. SVAP32 eliminated these effects by disrupting TGF-β1 binding to its receptors and blocking Ang II/TGF-β1 downstream signaling. These results demonstrated that sVAP32 has anti-fibrotic effects by blocking the TGF-β1 pathway in cardiac fibroblasts.

  18. Anti-inflammatory effects of enzymatic hydrolysates of velvet antler in RAW 264.7 cells in vitro and zebrafish model

    Science.gov (United States)

    Lee, Seung-Hong; Yang, Hye-Won; Ding, Yuling; Wang, Yanmei; Jeon, You-Jin; Moon, Sang-Ho; Jeon, Byong-Tae; Sung, Si-Heung

    2015-01-01

    Enzymatic hydrolysis has been successfully used for the extraction of numerous biologically active components from a wide variety of natural sources. In the present study, velvet antler was subjected to the extraction process using Alcalase protease. We analyzed bioactive components, such as uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs), and sialic acid, present in the velvet antler Alcalase hydrolysate (VAAH) and assessed their anti-inflammatory effects in zebrafish as well as in vitro using cell lines. VAAH mainly contained uronic acid (78.22 mg/g) and sulfated-GAGs (50.47 mg/g), while the amount of sialic acid was negligible (5.55 mg/g). VAAH inhibited the production of nitric oxide (NO) by lipopolysaccharide (LPS)-induced cells in a dose-dependent manner and the inhibitory effect of VAAH on NO production was higher than that of hot water extracts. VAAH treatment also reduced the expression of inflammatory mediators such as nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, we evaluated anti-inflammatory effects of VAAH using LPS-stimulated zebrafish. Treatment with LPS significantly increased cell death, NO, and reactive oxygen species (ROS) levels in zebrafish. Notably, VAAH significantly inhibited the extent of LPS-stimulated cell death and generation of NO and ROS in zebrafish. These results suggest that VAAH alleviated inflammation and cell death by inhibiting the generation of ROS induced by LPS treatment. Thus, VAAH could be used as a potential natural remedy with a strong anti-inflammatory effect. Taken together, we believe that based on our present results, enzymatic hydrolysis of velvet antler may be an effective process to make antler products acceptable as elements of health foods and nutraceutical components with increased biological activity. PMID:27152107

  19. Elemental Analysis of Bone, Teeth, Horn and Antler in Different Animal Species Using Non-Invasive Handheld X-Ray Fluorescence

    OpenAIRE

    Kittisak Buddhachat; Sarisa Klinhom; Puntita Siengdee; Brown, Janine L.; Raksiri Nomsiri; Patcharaporn Kaewmong; Chatchote Thitaram; Pasuk Mahakkanukrauh; Korakot Nganvongpanit

    2016-01-01

    Mineralized tissues accumulate elements that play crucial roles in animal health. Although elemental content of bone, blood and teeth of human and some animal species have been characterized, data for many others are lacking, as well as species comparisons. Here we describe the distribution of elements in horn (Bovidae), antler (Cervidae), teeth and bone (humerus) across a number of species determined by handheld X-ray fluorescence (XRF) to better understand differences and potential biologic...

  20. 马鹿茸多肽的提取分离及基质辅助激光解吸飞行时间质谱分析%Separation and Analysis of Velvet Antler Polypeptide by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    季怡萍; 张红明; 朱文彬

    2004-01-01

    In this paper, active components of velvet antler polypeptide were extracted and separated. The molecular weight and purity of velvet antler polypeptide were determined by MALDI-TOFMS. The different influence factors such as matrix, sample concentration and laser energy were studied. This method is convenient and suitable for the identification of congener biochemical samples.

  1. The Effects of Elk Velvet Antler Dietary Supplementation on Physical Growth and Bone Development in Growing Rats

    Directory of Open Access Journals (Sweden)

    Jiongran Chen

    2015-01-01

    Full Text Available Elk velvet antler (EVA has been used in traditional Oriental medicine for centuries to promote general health; however, little evidence for its effect on bone development is available. We investigated the effects of lifelong exposure of Wistar rats to a diet containing 10% EVA on physical growth and bone development. Measurements included weekly body weights, blood chemistry and kidney and testis/ovary indices (sacrificed at 5, 9, or 16 weeks of age, and bone traits of the femur bones by peripheral quantitative computed tomography (pQCT. Mean body weights were higher in the EVA group at 4–8 weeks in males and at 5 weeks of age in females. The kidney indices were greater in EVA dietary supplemented male rats at 5 and 16 weeks of age, in females at 16 weeks of age, and testis/ovary indices at 5 weeks of age. The femoral length was increased in both males and females at 5 weeks, and several pQCT-measured parameters had increased in EVA males and females. The activity of alkaline phosphatase (ALP increased in EVA group while the content of calcium and phosphorus did not differ among groups. Our results seem to support a role for dietary supplementation of EVA on growth and bone development in this model.

  2. The Effects of Elk Velvet Antler Dietary Supplementation on Physical Growth and Bone Development in Growing Rats.

    Science.gov (United States)

    Chen, Jiongran; Yang, Yanfei; Abbasi, Sepideh; Hajinezhad, Daryoush; Kontulainen, Saija; Honaramooz, Ali

    2015-01-01

    Elk velvet antler (EVA) has been used in traditional Oriental medicine for centuries to promote general health; however, little evidence for its effect on bone development is available. We investigated the effects of lifelong exposure of Wistar rats to a diet containing 10% EVA on physical growth and bone development. Measurements included weekly body weights, blood chemistry and kidney and testis/ovary indices (sacrificed at 5, 9, or 16 weeks of age), and bone traits of the femur bones by peripheral quantitative computed tomography (pQCT). Mean body weights were higher in the EVA group at 4-8 weeks in males and at 5 weeks of age in females. The kidney indices were greater in EVA dietary supplemented male rats at 5 and 16 weeks of age, in females at 16 weeks of age, and testis/ovary indices at 5 weeks of age. The femoral length was increased in both males and females at 5 weeks, and several pQCT-measured parameters had increased in EVA males and females. The activity of alkaline phosphatase (ALP) increased in EVA group while the content of calcium and phosphorus did not differ among groups. Our results seem to support a role for dietary supplementation of EVA on growth and bone development in this model. PMID:26366186

  3. Elemental Analysis of Bone, Teeth, Horn and Antler in Different Animal Species Using Non-Invasive Handheld X-Ray Fluorescence.

    Science.gov (United States)

    Buddhachat, Kittisak; Klinhom, Sarisa; Siengdee, Puntita; Brown, Janine L; Nomsiri, Raksiri; Kaewmong, Patcharaporn; Thitaram, Chatchote; Mahakkanukrauh, Pasuk; Nganvongpanit, Korakot

    2016-01-01

    Mineralized tissues accumulate elements that play crucial roles in animal health. Although elemental content of bone, blood and teeth of human and some animal species have been characterized, data for many others are lacking, as well as species comparisons. Here we describe the distribution of elements in horn (Bovidae), antler (Cervidae), teeth and bone (humerus) across a number of species determined by handheld X-ray fluorescence (XRF) to better understand differences and potential biological relevance. A difference in elemental profiles between horns and antlers was observed, possibly due to the outer layer of horns being comprised of keratin, whereas antlers are true bone. Species differences in tissue elemental content may be intrinsic, but also related to feeding habits that contribute to mineral accumulation, particularly for toxic heavy metals. One significant finding was a higher level of iron (Fe) in the humerus bone of elephants compared to other species. This may be an adaptation of the hematopoietic system by distributing Fe throughout the bone rather than the marrow, as elephant humerus lacks a marrow cavity. We also conducted discriminant analysis and found XRF was capable of distinguishing samples from different species, with humerus bone being the best source for species discrimination. For example, we found a 79.2% correct prediction and success rate of 80% for classification between human and non-human humerus bone. These findings show that handheld XRF can serve as an effective tool for the biological study of elemental composition in mineralized tissue samples and may have a forensic application. PMID:27196603

  4. Elemental Analysis of Bone, Teeth, Horn and Antler in Different Animal Species Using Non-Invasive Handheld X-Ray Fluorescence.

    Directory of Open Access Journals (Sweden)

    Kittisak Buddhachat

    Full Text Available Mineralized tissues accumulate elements that play crucial roles in animal health. Although elemental content of bone, blood and teeth of human and some animal species have been characterized, data for many others are lacking, as well as species comparisons. Here we describe the distribution of elements in horn (Bovidae, antler (Cervidae, teeth and bone (humerus across a number of species determined by handheld X-ray fluorescence (XRF to better understand differences and potential biological relevance. A difference in elemental profiles between horns and antlers was observed, possibly due to the outer layer of horns being comprised of keratin, whereas antlers are true bone. Species differences in tissue elemental content may be intrinsic, but also related to feeding habits that contribute to mineral accumulation, particularly for toxic heavy metals. One significant finding was a higher level of iron (Fe in the humerus bone of elephants compared to other species. This may be an adaptation of the hematopoietic system by distributing Fe throughout the bone rather than the marrow, as elephant humerus lacks a marrow cavity. We also conducted discriminant analysis and found XRF was capable of distinguishing samples from different species, with humerus bone being the best source for species discrimination. For example, we found a 79.2% correct prediction and success rate of 80% for classification between human and non-human humerus bone. These findings show that handheld XRF can serve as an effective tool for the biological study of elemental composition in mineralized tissue samples and may have a forensic application.

  5. Spin and chirality effects in antler-topology processes at high energy e{sup +}e{sup -} colliders

    Energy Technology Data Exchange (ETDEWEB)

    Choi, S.Y. [Chonbuk National University, Department of Physics, Jeonbuk (Korea, Republic of); University of Pittsburgh, Department of Physics and Astronomy, Pittsburgh Particle physics, Astrophysics, and Cosmology Center, Pittsburgh, PA (United States); Christensen, N.D. [Illinois State University, Department of Physics, Normal, IL (United States); Salmon, D.; Wang, X. [University of Pittsburgh, Department of Physics and Astronomy, Pittsburgh Particle physics, Astrophysics, and Cosmology Center, Pittsburgh, PA (United States)

    2015-10-15

    We perform a model-independent investigation of spin and chirality correlation effects in the antler-topology processes e{sup +}e{sup -} → P{sup +}P{sup -} → (l{sup +}D{sup 0})(l{sup +} anti D{sup 0}) at highenergy e{sup +}e{sup -} colliders with polarized beams. Generally the production process e{sup +}e{sup -} → P{sup +}P{sup -} can occur not only through the s-channel exchange of vector bosons, V{sup 0}, including the neutral Standard Model (SM) gauge bosons, γ and Z, but also through the s- and t-channel exchanges of new neutral states, S{sup 0} and T{sup 0}, and the u-channel exchange of new doubly charged states, U{sup --}. The general set of (nonchiral) three-point couplings of the new particles and leptons allowed in a renormalizable quantum field theory is considered. The general spin and chirality analysis is based on the threshold behavior of the excitation curves for P{sup +}P{sup -} pair production in e{sup +}e{sup -} collisions with longitudinal- and transverse-polarized beams, the angular distributions in the production process and also the production-decay angular correlations. In the first step, we present the observables in the helicity formalism. Subsequently, we show how a set of observables can be designed for determining the spins and chiral structures of the new particles without any model assumptions. Finally, taking into account a typical set of approximately chiral invariant scenarios, we demonstrate how the spin and chirality effects can be probed experimentally at a high-energy e{sup +}e{sup -} collider. (orig.)

  6. Spin and chirality effects in antler-topology processes at high energy e{sup +}e{sup -} colliders

    Energy Technology Data Exchange (ETDEWEB)

    Choi, S. Y. [Department of Physics, Chonbuk National University, 561-756, Jeonbuk (Korea, Republic of); Department of Physics and Astronomy, Pittsburgh Particle physics, Astrophysics, and Cosmology Center, University of Pittsburgh, 15260, Pittsburgh, PA (United States); Christensen, N. D. [Department of Physics, Illinois State University, 61790, Normal, IL (United States); Salmon, D.; Wang, X., E-mail: xiw77@pitt.edu [Department of Physics and Astronomy, Pittsburgh Particle physics, Astrophysics, and Cosmology Center, University of Pittsburgh, 15260, Pittsburgh, PA (United States)

    2015-10-06

    We perform a model-independent investigation of spin and chirality correlation effects in the antler-topology processes e{sup +}e{sup -}→P{sup +}P{sup -}→(ℓ{sup +}D{sup 0})(ℓ{sup -}D{sup -bar0}) at high-energy e{sup +}e{sup -} colliders with polarized beams. Generally the production process e{sup +}e{sup -}→P{sup +}P{sup -} can occur not only through the s-channel exchange of vector bosons, V{sup 0}, including the neutral Standard Model (SM) gauge bosons, γ and Z, but also through the s- and t-channel exchanges of new neutral states, S{sup 0} and T{sup 0}, and the u-channel exchange of new doubly charged states, U{sup --}. The general set of (non-chiral) three-point couplings of the new particles and leptons allowed in a renormalizable quantum field theory is considered. The general spin and chirality analysis is based on the threshold behavior of the excitation curves for P{sup +}P{sup -} pair production in e{sup +}e{sup -} collisions with longitudinal- and transverse-polarized beams, the angular distributions in the production process and also the production-decay angular correlations. In the first step, we present the observables in the helicity formalism. Subsequently, we show how a set of observables can be designed for determining the spins and chiral structures of the new particles without any model assumptions. Finally, taking into account a typical set of approximately chiral invariant scenarios, we demonstrate how the spin and chirality effects can be probed experimentally at a high-energy e{sup +}e{sup -} collider.

  7. Deer Antler Extract Improves Fatigue Effect through Altering the Expression of Genes Related to Muscle Strength in Skeletal Muscle of Mice

    Directory of Open Access Journals (Sweden)

    Jaw-Chyun Chen

    2014-01-01

    Full Text Available Deer antler is a well-known traditional Chinese medicine used in Asian countries for the tonic and the improvement of aging symptoms. The present study was designed to investigate the antifatigue effect and mechanism of Formosan sambar deer tip antler extract (FSDTAE. The swimming times to exhaustion of mice administered FSDTAE (8.2 mg/day for 28 days were apparently longer than those of the vehicle-treated mice in forced swim test. However, the indicators of fatigue, such as the reduction in glucose level and the increases in blood urea nitrogen and lactic acid levels, were not significantly inhibited by FSDTAE. Therefore, microarray analysis was further used to examine the anti-fatigue mechanism of FSDTAE. We selected genes with fold changes >2 or <−2 in skeletal muscle for pathway analysis. FSDTAE-affected genes were involved in 9 different signaling pathways, such as GnRH signaling pathway and insulin signaling pathway. All of the significantly expressed genes were classified into 8 different categories by their functions. The most enriched category was muscular system, and 6 upregulated genes, such as troponin I, troponin T1, cysteine and glycine-rich protein 2, myosin heavy polypeptide 7, tropomyosin 2, and myomesin family member 3, were responsible for the development and contraction of muscle. Real-time PCR analysis indicated that FSDTAE increased troponins mRNA expression in skeletal muscle. In conclusion, our findings suggested that FSDTAE might increase the muscle strength through the upregulation of genes responsible for muscle contraction and consequently exhibited the anti-fatigue effect in mice.

  8. Effects and efficiency of consolidation treatments on Palaeolithic reindeer antler. Multi-analytical study by means of XRD, FT-IR microspectroscopy, SEM, TEM and μ-PIXE/PIGE analyses

    Science.gov (United States)

    Chadefaux, C.; Vignaud, C.; Menu, M.; Reiche, I.

    2008-07-01

    Bone materials record information on palaeodiets and palaeoenvironment, contained in their chemical and isotopic composition. Therefore, it is very important, while preserving these objects, to keep an open access to this information. In the present paper, we characterise the effects of consolidation treatments on the structure and chemical composition of archaeological reindeer antler at different hierarchical scales. X-ray diffraction, infra-red spectroscopy and microscopy, scanning and transmission electron microscopy coupled with an X-ray analysing system and proton-induced X-ray and γ-ray emission were used to reveal the state of preservation of reindeer antler and to evaluate the effectiveness of their consolidation treatments. These treatments are shown not to affect the chemical composition, but the crystallinity of the solid phase, as far as Palaeolithic material is concerned. In terms of penetration, the most efficient treatment, among those tested, seems to be the Rhodopas 15% single-bath treatment.

  9. Effect of the Velvet Antler of Formosan Sambar Deer (Cervus unicolor swinhoei on the Prevention of an Allergic Airway Response in Mice

    Directory of Open Access Journals (Sweden)

    Ching-Yun Kuo

    2012-01-01

    Full Text Available Two mouse models were used to assay the antiallergic effects of the velvet antler (VA of Formosan sambar deer (Cervus unicolor swinhoei in this study. The results using the ovalbumin- (OVA- sensitized mouse model showed that the levels of total IgE and OVA-specific IgE were reduced after VA powder was administrated for 4 weeks. In addition, the ex vivo results indicated that the secretion of T helper cell 1 (Th1, regulatory T (Treg, and Th17 cytokines by splenocytes was significantly increased (P<0.05 when VA powder was administered to the mice. Furthermore, OVA-allergic asthma mice that have been orally administrated with VA powder showed a strong inhibition of Th2 cytokine and proinflammatory cytokine production in bronchoalveolar fluid compared to control mice. An increase in the regulatory T-cell population of splenocytes in the allergic asthma mice after oral administration of VA was also observed. All the features of the asthmatic phenotype, including airway inflammation and the development of airway hyperresponsiveness, were reduced by treatment with VA. These findings support the hypothesis that oral feeding of VA may be an effective way of alleviating asthmatic symptoms in humans.

  10. 鹿茸顶端组织端粒酶活性检测及TERT基因的差异表达%Detecting Telomerase Activity and Differential Expression Analysis of TERT Gene in Antler Tip Tissue

    Institute of Scientific and Technical Information of China (English)

    胡薇; 李沐; 李婷; 田玉华; 孟星宇; 刘宁

    2013-01-01

    为了检测梅花鹿鹿茸顶端组织不同部位的端粒酶活性及端粒酶逆转录酶( TERT )基因的mRNA表达水平,分离鹿茸顶端组织的茸皮层、间充质层及软骨层,并进行细胞培养,利用TRAP银染法测定不同部位的端粒酶活性。再利用TRIzol试剂法分别提取茸皮层、间充质和软骨细胞的总RNA,逆转录合成cDNA。根据Gen-Bank已发表的相关序列设计梅花鹿TERT基因部分序列特异引物并克隆TERT基因,采用相对荧光定量Real-time PCR法检测TERT在鹿茸顶端不同部位细胞的表达丰度。结果表明:在鹿茸顶端组织茸皮层、间充质层和软骨层均检测到了端粒酶活性,其中间充质层的端粒酶活性最高,软骨层次之,茸皮层端粒酶活性最低;而体外培养的不同代数间充质细胞的端粒酶活性无明显差异。成功获得了鹿茸组织TERT基因部分编码区cDNA序列,该基因长915 bp,编码305个AA;与牛、羊TERT基因进行同源性分析显示,核苷酸序列分别为94.89%和94.31%;氨基酸序列分别为92.16%和92.11%。相对荧光定量PCR差异分析发现,TERT基因在鹿茸顶端不同部位组织均有表达。其中,在间充质组织的表达水平高于软骨和茸皮层组织。%In order to detect the telomerase activity of different parts of top tissue of sika deer antler and mRNA expression of TERT gene, the skin of antler, mesenchymal layer and layer of cartilage were separated from the antler tip tissue and cul-tured.TRAP silver staining was improved to detect the telomerase activity of different parts.And total RNA of cartilage layer and the skin layer of antler and mesenchymal layer were isolated by TRIzol reagent to use reverse transcription for synthesizing the cDNA.According to the correlation sequences that GenBank published, sika TERT gene partial sequence special primers were designed and the gene was cloned.The relative fluorescence

  11. 梅花鹿三种深加工制品的营养保健成分研究%Studies on Nutritious Health- Protecting Component of Wine from Pilose Antler Blood Deer's Heart Flake and deer's Penis Pantocrin

    Institute of Scientific and Technical Information of China (English)

    董万超; 赵伟刚; 张秀莲; 张宝香; 赵立波; 赵景辉

    2001-01-01

    This test hasanalysed and determinal the contents for protein, grease, polysaccharide, Vitamin and inorganic element of the wine from pilose antler, deer's heart flake and deer's penis pantocrin.The test has obviously showed that three products of that are good for nutritious health - protecting function after experiment.%分析测定了鹿茸血酒,鹿心片和鹿鞭精中的蛋白质、脂肪、总糖、维生素及无机微量元素的含量。结果表明,它们是优良的营养保健佳品。

  12. 鹿茸多糖分离纯化及抗氧化活性研究%Purification and antioxidant activity of polysaccharide from velvet antler

    Institute of Scientific and Technical Information of China (English)

    赵玉红; 金秀明; 韩睿

    2012-01-01

    采用DEAE-52离子交换层析和Sepharose CL-6B凝胶排阻层析对鹿茸粗多糖进行分离纯化,并通过对DPPH自由基、羟基自由基(·OH)、超氧阴离子自由基(O2-·)清除能力和还原能力的测定,研究了粗多糖及纯化后多糖的抗氧化能力。结果表明:DEAE-52离子交换层析和Sepharose CL-6B凝胶排阻层析对鹿茸多糖分离效果较好,可以分离纯化得到一种单一多糖;粗多糖和纯化后多糖对DPPH·、·OH、O2-·均有清除作用,且具有一定的还原能力,纯化后多糖抗氧化活性和还原能力均大于粗多糖。%DEAE-52 ion-exchange column chromatography and Sepharose CL-6B gel exclusion chromatography were used to isolate the crude polysaccharide.Antioxidant activities of the crude and purified polysaccharide were evaluated through their capability of scavenging DPPH·,·OH,O-2· radicals and reducing power.The result showed that,DEAE-52 ion-exchange column chromatography and Sepharose CL-6B gel exclusion chromatography were suitable for separating and purifying polysaccharide from velvet antlers,a kind of purified polysaccharide was isolated from the crude polysaccharide.Both of the polysaccharide crude and purified had significant effect on scavenging DPPH·,·OH,O-2· radicals and also had reduction ability.Furthermore purified polysaccharide had more activity antioxidant than crude polysaccharide.

  13. Antilipid peroxidation of polyamines from pilose antler%鹿茸多胺的抗脂质过氧化作用

    Institute of Scientific and Technical Information of China (English)

    陈晓光; 金淑莉; 邸琳; 刘新宇; 张晓宇

    2004-01-01

    目的研究鹿茸多胺的抗氧化作用.方法测定鹿茸多胺在体外对NADPH-维生素C和Fe 2+-半胱氨酸系统诱发的微粒体脂质过氧化反应(MDA形成)的影响,对黄嘌呤-黄嘌呤氧化酶系统超氧阴离子自由基(O-2)产生(还原型细胞色素C形成)的影响,在体内对CCl4和乙醇诱发的小鼠肝脂质过氧化反应(MDA形成)的影响.结果鹿茸多胺在体外能明显抑制NADPH-维生素C和Fe2+-半胱氨酸系统诱发的大鼠脑、肝、肾微粒体脂质过氧化反应(MDA形成),及黄嘌呤-黄嘌呤氧化酶系统O-2的产生(还原型细胞色素C形成).在体内能抑制CCl4和乙醇诱发的小鼠肝脂质过氧化反应(MDA形成).结论鹿茸多胺具有抗氧化作用.%Object To investigate the antioxidant activity of polyamines isolated from pilose antler(paipa).Mthods The effects of PAIPA ON the lipid peroxidation(MDA formation)in microsomes of rat brain,liver ,and kidney induced by NADPH-Vitamine C(Vc)and ferrous-cysteine systems in vitro,the superoxide anion radical production(reduced cytochrome C formation)inxanthine-xanthine oxidase system in vitro,and the CCL4-and ethanol-induced MDA formation in mice liver in vivo were evaluated.Results PAIPA could significantly inhibit the lipid peroxidation(MDA formation)in microsomes of rat brain,liver,and kidney induced by NADPH-Vc and ferrous -cyctein,the superoxide anion radical produc-tion(formation of reduced cytochrome C)in xanthine-oxidase system in vitro,and the CCL4-and ethanol-induced MDA formation in mice liver in vivo.Conclusion PAIPA exhibits an antioxidant activity.

  14. 麋鹿角醇提取液改善衰老小鼠免疫功能%Elk antlers ethanolic extract improves immunological function of aging mice

    Institute of Scientific and Technical Information of China (English)

    秦红兵; 杨朝晔; 于广华; 朱清

    2009-01-01

    Objective To investigate the effect of Elk antlers ethanolic extract (EAEE) on immunological function of aging mice. Methods Fifty-four mice were randomly divided into 4 groups of normal controKgroup NG, 12 mice) and three aging model groups(MG, LG and HG) with 14 mice each. The aging model was induced by D-galactose(D-gal) 120 mg/kg per day for six weeks. During oral administration of D-gal, EAEE 2 g· Kg~(-1)21·~(-1) was given in group HG and 4 g · kg~(-1)·d~(-1) in group LG. Some parameters related to immunological function were examined with MTT, ELISA and RT-PCR methods. Results The D-gal induced aging mice model, which was characterized by depression, loss of appetite, weight loss, pelage losing sheen, lacking of skin elasticity and so on. The serum IgG level, lymphocyte transforming SI of the spleen, serum concentration of interferon-gama (IFN-γ), IFN-γ mRNA and interleukin-2(IL-2) were reduced in aging models of group MG, which were all increased in group HG. Conclusion EAEE improves cellular and humoral immunological function of the aging model of mice induced by D-gal.%目的 观察麋鹿角醇提液对D-半乳糖(D-gal)诱导衰老模型小鼠免疫功能的影响.方法 54只小鼠随机分成正常对照(NG)组和三个D-gal诱导衰老模型(MG、LG、HG)组.在造模同时,LG、HG两组分别给予麋鹿角醇提液2 g/kg和4 g/kg.测定部分免疫相关的血清指标.结果 小鼠经D-gal处理6周后出现衰老体征.MG组血清IgG水平下降,脾淋巴细胞转化刺激指数下降,血清IFN-γ浓度IFN-γ mRNA表达水平下降.HG组小鼠IgG水平、脾淋巴细胞转化刺激指数、血清中IL-2和IFN-γ的浓度升高,并增强模型鼠脾淋巴细胞IL-2、IFN-γ mRNA的表达.结论 麋鹿角醇提液能增强D-gal诱导的衰老模型小鼠体液免疫及细胞免疫功能.

  15. Construction of Full-length cDNA Library for Antler Tip Tissue of Sika Deer%东北梅花鹿鹿茸尖端组织全长cDNA文库的构建

    Institute of Scientific and Technical Information of China (English)

    郝丽; 李和平; 严厉

    2009-01-01

    为克隆出与鹿茸生长发育相关基因的全长序列,采用SMART技术构建了东北梅花鹿鹿茸尖端组织的全长cDNA文库.用SV Total RNA Isolation System试剂盒提取总RNA,以逆转录酶PowerScriptTM 反转录合成第一链cDNA,然后通过LD-PCR合成并扩增ds cDNA.扩增产物经纯化、SfiⅠ酶切、过CHROMA SPIN-400柱去除小片段后,连接到SfiⅠ消化过的pDNR-LIB质粒载体中,最后用电转化法将重组质粒转化到E. coli DH5α内得到原始文库.经测定,构建的原始文库约含有2.56×10~6个重组子,插入片段多在0.5~2kb之间,平均插入片段长度约1.1kb,重组效率接近100%.结果表明,东北梅花鹿鹿茸尖端组织的全长cDNA文库已构建成功.%A study was conducted to construct full-length cDNA library from antler tip tissues of Sika Deer (Cenna nippon hortu-lonun) by SMART technique in order to clone new special genes for development of antler. The total RNA was extracted u-sing SV Total RNA Isolation System. Single-stranded cDNA was synthesized using PowerScripiTM reverse transcriptase,and double-stranded cDNA was synthesized and amplified by long-distance PCR. The PCR products were digested by pro-teinase K and purified. After digestion with Sfi I and size fractionation using CHROMA SPIN -400TM Columns, SMART cDNA was ligated to the Sfi I-digested, dephosphorylated pDNR-LIB vector, and the ligation mixture was transformed into E. call DH5a by electroporation. The primary cDNA library contained 2.56×10~6 independent clones with DNA inserts of 0.5~2. 0 kb, the average size of inserted cDNAs was 1.1 kb, and the recombination percentage was about 100%. Results showed that the full-length cDNA library from antler tip tissues of Sika Deer was successfully constructed.

  16. 鹿茸提取物对顺铂诱导人胚肾细胞损伤的影响∗%Effect of Velvet Antler on Human Embryonic Kidney Cell Injury In-duced by Cisplatin

    Institute of Scientific and Technical Information of China (English)

    王婧瑜; 王露露; 李冰; 刘芳芳; 孙长波; 张晶

    2015-01-01

    This experiment was designed to study the effects of pilose antler extractions ( water ex⁃tract, ethanol extract, ether extract, chloroform extract and ethyl acetate extract) to protect HEK293 injury induced by cisplatin( CDDP ) . HEK293 was cultured in vitro and the results were determined by MTT and LDH. Pilose antler extractions can protect CDDP⁃induced injury of HEK293, and the death rate was more than half at the level of (0�083±0�030) mmol/L. After HEK293 cells were in⁃cubated for 24 h with different concentrations of extract of pilose antler, CDDP was joined respec⁃tively. It was found that water extraction has better protection on CDDP⁃induced HEK293, and at the level of 1 mg/mL, the survival rate of cells was 62%( P<0�01) . Water extraction can effectively protect CDDP⁃induced HEK293 and maintain cell activity.%建立顺铂(CDDP)诱导人胚肾成纤维细胞(HEK293)细胞损伤模型,通过MTT和LDH测定,比较不同浓度的鹿茸提取物(水提物,醇提物,乙醚提取物,氯仿提取物,乙酸乙酯提取物)诱导HEK293细胞损伤的保护作用。体外培养HEK293细胞,观察鹿茸提取物对CDDP诱导HEK293细胞生长的影响,并用MTT法和LDH法检测鹿茸对CDDP诱导HEK293细胞死亡率的变化。结果表明:鹿茸提取物能够拮抗CDDP诱发的细胞损伤,具有浓度依赖性,其半数致死浓度为(0�083±0�030) mmol/L。当HEK293细胞与不同浓度鹿茸提取物共同孵育24 h,分别加入CDDP后,检测表明鹿茸水提物较其他溶剂提取物对顺铂诱导损伤的HEK293有较好的保护作用,其质量浓度为1 mg/mL时对顺铂诱导损伤的HEK293细胞的保护作用最强,细胞存活率达到62�0%( P<0�01)。鹿茸水提物对CDDP诱导人胚肾细胞损伤的抑制作用最显著,可有效保护细胞活性。

  17. 不同部位鹿茸水提液对NRK-49F细胞促增殖作用的研究%Comparative Analysis of Promoting Effects on NRK-49F Cells Proliferation by Different Sections of Velvet Antler Water Extracts

    Institute of Scientific and Technical Information of China (English)

    吴帆; 董玲; 王春梅; 丁倩男; 刘建亭; 赓迪; 戴俊东

    2014-01-01

    目的:研究鹿茸不同部位水提液体外对细胞促增殖作用的差异。方法:以大鼠肾成纤维细胞NRK-49F作为模型,以MTT法检测鲜鹿茸上、中、下三部分水提液的促细胞增殖率,以BCA法检测样品蛋白浓度,SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)方法分析样品蛋白组成的差异。结果:上、中、下部鹿茸样品可溶性蛋白含量分别为17.89、16.04和6.89 mg·mL-1,从顶端到基部,鹿茸可溶性蛋白的含量依次降低。加样24 h时,鹿茸上部和中部样品分别在蛋白浓度800μg·mL-1和600μg·mL-1时达到最大促增殖率,分别为66.76%和64.36%,下部样品在1000μg·mL-1时达到最大促增殖率,为58.87%。作用48 h后,鹿茸上部和中部样品均在蛋白浓度800μg·mL-1时达到最大促增殖率,分别为219.56%和215.86%,下部样品在蛋白浓度1000μg·mL-1时达到最大促增殖率,为169.20%。鹿茸样品对细胞的增殖促进作用远大于10%胎牛血清。3个部位样品蛋白组成的SDS-PAGE图谱差异不大。结论:所有样品对细胞增殖均有促进作用,且呈现浓度依赖效应。不同部位样品主要蛋白组成差异不大。%This study was aimed to explore differential effects of various sections of the velvet antler on promoting cell proliferation in vitro. The NRK-49F cell line from rat kidney fibroblasts was used as the cell model. The cell proliferation rates of the water extracts from the upper, middle and lower section of fresh velvet antler were measured by the MTT method. BCA method was used in the detection of protein concentration. The SDS-PAGE method was used in the analysis of difference composition of the sample protein. The results showed that soluble protein content of the upper, middle and lower section were 17.89, 16.04 and 6.89 mg·mL-1, respectively. From the top to the base, the soluble protein content of velvet antler was decreased. After 24 h treatment, when the protein

  18. Effect of antler velvet alcohol extracts on improving learning and memory sbilities in mice%鹿茸醇提物对小鼠学习记忆功能的影响

    Institute of Scientific and Technical Information of China (English)

    赵玉红; 张睿; 潘强

    2012-01-01

    Objective:To investigate the effect of antler velvet alcohol extract(AVAE)on learning and memory in mice.Methods:One hundred and fifty-six male Kunming mice were randomized into control group(12 mice,given salty water),memory obtain stage group,memory consolidate stage group and memory reappearance stage group(48 mice in each group),each group had 3 small groups:high dose,mid dose and low dose group,given alcohol extract of antler velvet dry matter on 1kg/(kg·d),0.5kg/(kg·d)and 0.1kg/(kg·d)respectively.After 30d,each stage's latency and number of errors of jumping platform experiment as well as swimming time and number of errors of water maze were tested,at the same time,the activity of SOD and the content of MDA in mind were evaluated.Results:The AVAE could prolong the latency(P0.01)of jumping platform experiment on each stage as well as decreasing the number of errors(P0.01)obviously,and decrease the swimming time and the number of errors(P0.01,P0.05)of water maze,then increase the activity of SOD(P0.01,P0.05)and decrease the content of MDA in mice brain(P0.01,P0.05).Conclusions:The AVAE had the function of improving learning and memory impairment obviously.%目的:研究鹿茸醇提物(AVAE)对小鼠学习记忆能力的影响。方法:156只昆明种雄性小鼠分成空白组(12只小鼠,灌胃生理盐水)、记忆获取阶段组、记忆巩固阶段组和记忆再现阶段组(每组48只小鼠),各阶段组中的高、中、低剂量组每天每只小鼠分别灌胃鹿茸乙醇提取物的干粉量为1kg/(kg·d)、0.5kg/(kg·d)和0.1kg/(kg·d)。灌胃30d,测定各个记忆阶段小鼠跳台实验的潜伏期及错误次数、水迷宫实验的游出时间及错误次数和小鼠大脑内的SOD酶活力及MDA含量。结果:鹿茸醇提物能明显延长各个阶段小鼠跳台实验的潜伏期(P〈0.01)并减少错误次数(P〈0.01);缩短小鼠水迷宫实

  19. Cloning of a Full-Length cDNA Encoding Insulin-Like Growth Factor I and Its Expression in Antler Tissue%梅花鹿IGF1全长cDNA克隆及在鹿茸组织的表达

    Institute of Scientific and Technical Information of China (English)

    胡薇; 孟星宇; 田玉华; 刘宁

    2011-01-01

    The study was to detect expression of Insulin-Like Growth Factor I(IGF1) from antler tip tissue to provide a theoretical basis for the molecular mechanism of antler growth. Total RNA was isolated from dermis, mesenchyme, pre-cartilage and cartilage layers in growing antler tip tissue respectively. A pair of primers was designed according to the homology region of IGF1 among other species. The cDNA sequence of 1GF1 gene was cloned by RT-PCR technology. Then, real-time PCR was used to detect the expression level of IGF1 in the antler tip. Results showed that the complete coding sequence of IGF1 gene was obtained (GenBank accession No: HQ890468). It has 465 base pairs in length, encoding a predicted protein of 154 amino acids. Bioinformatic analysis based on IGF1 genes of red deer, cattle, sheep, horse, pig, human and mouse showed that the homologies of ICF1 to red deer, cattle and sheep were 99.78% , 98.28% and 97. 85% , and the homologies of protein were 99. 35% , 98. 05% and 97. 40% respectively. Results of fluorescence quantification showed that IGF1 was expressed in all the tested organs in antler tip tissue, but its transcript level in cartilage and pre-cartilage layers was much higher than that in mesenchyme and dermis layers.%为了检测梅花鹿鹿茸组织IGF1基因的mRNA表达水平,利用TRIzol试剂法分别提取鹿茸真皮、间充质、前软骨和软骨组织总RNA,逆转录合成cDNA.根据GenBank已发表的相关序列设计梅花鹿IGFI基因特异引物并克隆IGFI基因,将IGF1基因DNA序列递交GenBank,并利用相对荧光定量Real-time PCR法检测IGF1在鹿茸顶端不同部位组织的转录表达丰度.研究结果表明:成功获得了梅花鹿鹿茸组织IGF1基因全长编码区cDNA(GenBank登录号为HQ890468),该基因长465 bp,编码154aa长度的多肽;与马鹿、牛、羊、马、猪、人和小鼠IGF1基因进行同源性分析显示,IGF1基因与马鹿、牛和羊同源性最高,核苷酸序列分别为99.78% 、98.28

  20. Simultaneous determination of eleven sex hormones in antler velvet health products by gas chromatography-tandem mass spectrometry%鹿茸保健品中11种性激素的气相色谱-串联质谱法分析

    Institute of Scientific and Technical Information of China (English)

    芦春梅; 王明泰; 牟俊; 卢利军; 周晓

    2011-01-01

    建立了气相色谱-串联质谱法测定鹿茸保健品中11种性激素的分析方法.鹿茸中的性激素经固相萃取富集和净化,经七氟丁酸酐衍生处理.采用DB-5色谱柱(30 m×0.25 mm,0.25μm)、非线性梯度升温程序分离,在串联质谱多反应监测(MRM)模式下检测,外标法定量,实现了11种性激素的有效分离.11种性激素的检出限为1.0~5.0μg/kg,线性相关系数为0.991 6~0.999 9,平均回收率为67.4%~99.1%,相对标准偏差为2.6%~13%.该方法准确,可靠,可满足鹿茸保健品中性激素含量的测定和确证.%A method for the simultaneous determination of 11 sex hormones in antler velvet health products by gas chromatography-tandem mass spectrometry ( GC-MS/MS) was developed. The sex hormones in antler velvet were enriched and purified by solid phase extraction and derivatized with heptafluorobutyric acid anhydride (HFBA). A DB-5 column (30 m xO. 25 mm, 0. 25 μm) with nonlinear gradient program was used in GC separation. The sex hormones were determined in the multiple reaction monitoring mode. The method realized the complete separation of 11 sex hormones. The limits of detection of this method were from 1.0 to 5. 0 (μg/kg for the 11 sex hormones. The correlation coefficients were between 0. 991 6 and 0. 999 9. The recoveries were in the range of 67. 4% -99. 1% with relative standard deviations (RSDs) of 2. 6% - 13%. This method is accurate and reliable for the determination of the sex hormones in antler velvet health products.

  1. Stud of Enhancement to Skin Wound Healing by Pilose Antler Polypeptides%鹿茸多肽促进皮肤创面愈合的基础研究

    Institute of Scientific and Technical Information of China (English)

    牛琼; 刘黎军

    2013-01-01

    目的:探讨鹿茸多肽对皮肤愈合的影响。方法:设计将48只大鼠随机分成1个对照组和3个实验组,每组12只。实验组1单纯肌肉注射用药;实验组2为单纯创口外部用药;实验组3则肌注与外部混合用药。以大鼠背部以脊柱为中心设计5cm*5cm创面,每日给药1次,分别于2、4、6、8、10、12、14日观察创面的愈合情况;于第10天测量创面大小、计算愈合面积、愈合速度;单位重量组织细菌计数;肉芽组织羟脯氨酸含量测定。结果:创面愈合面积、愈合速度和肉芽组织羟脯氨酸含量测定实验组3优于其它3个实验组,而单位重量组织细菌计数低于其它3个实验组。结论:鹿茸多肽能够提高皮肤愈合速度,增加组织细胞间连接,加速上皮化生。%Objective:To discuss the influence of Pilose antler polypeptide(PAP) to skin healing. Methods:Divide 48 rabbits into 4 groups at random, one contrast group and three experimental groups. Only PAP injection in experimental group 1;only external use of PAP in experimental group 2;mixed usage of PAP in experimental group 3. Design a wound of 5 cm in diameter around spine on the back of rabbit. Use medicine once a day and observe growth of the wound on 2、4、6、8、10、12、14 days. Measure wound area, calculate healing area and velocity on 10th day, exam amount of bacteria and hydroxyproline. Results:Healing area, velocity and containing of hydroxyproline of experimental group 3 are higher than other three groups. Amount of bacteria are lower than other three groups. Conclusion:PAP can enhance skin wound healing, increase connection of cells and tissue, accelerate growth of epithelial tissue.

  2. Combination of Supercritical Fluid Extraction with Ultrasonic Extraction for Obtaining Sex Hormones and IGF-1 from Antler Velvet%超临界萃取技术结合超声强化技术提取鹿茸中性激素和IGF-1

    Institute of Scientific and Technical Information of China (English)

    周冉; 李淑芬; 张大成

    2009-01-01

    Supercritical CO2 (SC-CO2) extraction technology and ultrasonic technology were used to extract two active sex hormones, estradiol and progesterone, and insulin-like growth factor-1 (IGF-1) from antler velvet. The effects of SC-CO2 extraction condition on the extraction yield and content of sex hormones, the ultrasonic extraction condition on the content of IGF-1 and the SC-CO2 extraction condition on the activity remaining of IGF-1 were studied. The optimal conditions were obtained. The experimental results velvet residue, when the pHIO ammonia-ammonium chloride buffer solution was used as the solvent, the ratio of solvent to sample was 20/1 (volume/mass), the extraction temperature was 0-35℃, and the extraction time was 4×15 min. Under these conditions, 93.68% activity remaining of IGF-1 in the residue was obtained, while little IGF-1 activity exists in traditional residue. The experimental results indicate that the technology of SC-CO2 with co-solvent is of advantage for getting high content sexual hormones and keeping high activity of IGF-1 in the residue, which can not be achieved by traditional extraction methods.

  3. Suvemaja, Antler Island, Fairy Lake, Ontario / Ants Elken

    Index Scriptorium Estoniae

    Elken, Ants, 1917-2012

    1997-01-01

    Tuli projekteerida suvila kasvavale perele, mis algusest peale kasutamiskõlblikuna areneks koos perekonna laienemisega. Kerged paviljonid meenutavad küla. Ehitati 1966-1989. Arhitekt Elmar Tampõld. Joonised, vaated.

  4. 麋鹿角的FTIR指纹图谱分析%The FTIR fingerprint analysis of Elk's antler

    Institute of Scientific and Technical Information of China (English)

    严加琴; 王丽娟; 周逸芝; 刘训红

    2013-01-01

    目的 建立麋鹿角傅立叶变换红外光谱(FTIR)指纹图谱分析方法.方法 用傅立叶变换红外光谱技术对不同年龄阶段不同部位的麋鹿角进行分析,测定其指纹图谱,并进行相似度评价.结果 初步建立了麋鹿角的对照FTIR指纹图谱.结论 该法准确可靠,重现性好,FTIR指纹图谱分析方法可用于麋鹿角药材的品质评价.

  5. 气流粉碎机超微粉碎鹿茸的研究%Research on Ultrafine Smashing Antler by Jet Mill

    Institute of Scientific and Technical Information of China (English)

    杨静; 张争明; 李伯廷; 秦金山

    2012-01-01

      应用TC型流化床超音速气流粉碎机组对鹿茸进行超微粉碎,研究了气流粉碎机的工艺参数对超微粉碎鹿茸效果的影响;研究了分级机转速、进气压力、进料粒度对鹿茸超微粉碎的影响,同时还研究了不同加工方法干燥的鹿茸和不同部位的鹿茸等药材本身的物料性能对粉碎效果的影响。用激光粒度分析仪测定其微粉的粒度分布。在设备进气压力1畅0MPa ,分级机反转2400r/min ,进料粒度80目的操作参数下,能得到烘干鹿茸微粉的中位粒径为18畅09μm ,冻干鹿茸微粉的中位粒径为30畅6μm;烘干茸较冻干茸易粉碎,鹿茸部位不同粉碎的效果不同。运用TC型流化床超音速气流粉碎机组实现并优化了鹿茸的超微粉碎。

  6. Comparative element analysis on femur of antler hemo-treated ovariectomized Wistar rats by SRXRF%鹿茸血处理前后去势大鼠股骨的SRXRF分析

    Institute of Scientific and Technical Information of China (English)

    杨建虹; 费聿荣; 曹毅; 王瑞林; 黄宇营; 何伟; 刘兢

    2007-01-01

    运用同步辐射X射线荧光(Synchrotron radiation X-ray fluorescence,SRXRF)技术分析鹿茸血处理前后的去势大鼠股骨元素含量及与骨密度(Bone mineral density,BMD)的关系.分别取出假手术组、去势组和去势后经鹿茸血处理组的雌性Wistar大鼠的左股骨,经SRXRF分析,结果显示:元素Ca、P、Zn和Sr之间有非常好的正相关性.与假手术组相比,去势大鼠股骨元素Ca、P、Zn和Sr的相对强度均有降低,其中元素Ca、P的相对强度明显降低(p<0.05),其BMD也显著降低(p<0.05),说明由于Ca和P元素的大量丢失,导致骨质疏松.用鹿茸血处理后,去势大鼠股骨元素Ca、P、Zn的相对强度都有不同程度的升高,因而其BMD也显著升高(p<0.05),骨质疏松得到显著恢复.结果表明:鹿茸血有助于维持骨组织中微量元素Zn的含量,进而保持骨Ca和P的含量,从而提高BMD,减少骨质疏松的发生.

  7. 77 FR 59646 - Notice of Inventory Completion: U.S. Department of Defense, Army Garrison, Redstone Arsenal...

    Science.gov (United States)

    2012-09-28

    ...: Notice is here given in accordance with the Native American Graves Protection and Repatriation Act... the museum, institution, or Federal agency that has control of the Native American human remains and...; 2 preforms; 1 rodent tooth; 1 beaver tooth; 1 deer antler tine; 1 drilled deer antler piece; 2...

  8. 马鹿茸多肽的提取分离及基质辅助激光解吸飞行时间质谱分析

    Institute of Scientific and Technical Information of China (English)

    季怡萍; 张红明; 朱文彬

    2004-01-01

    In this paper, active components of velvet antler polypeptide were extracted and separated. The molecular weight and purity of velvet antler polypeptide were determined by MALDI-TOFMS. The different influence factors such as matrix, sample concentration and laser energy were studied. This method is convenient and suitable for the identification of congener biochemical samples.

  9. A Novel Polypeptide from Cervus elaphus Linnaeus

    Institute of Scientific and Technical Information of China (English)

    LiangWENG; QiuLiZHOU; 等

    2002-01-01

    A novel polypeptide having stimulant effect on some cell proliferation was isolated from the velvet antler (Cervus elaphus Linnaeus). The velvet antler polypeptide consists of a single chain of 32 amino acid residues. Amino acid sequence of the polypeptide was identified as:VLSAADKSNVKAAWGKVGGNAPAFGAEALLRM.

  10. Hillside, Morgans Brake, and Mathews Brake National Wildlife Refuges Deer Harvest Records are from 2008 and 2014

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — These harvest data are from Hillside, Morgans Brake, and Mathews Brake NWRs during the 2008 and 2014 deer seasons. They measure weight, antler size, prevalence of...

  11. Deer Management Assistance Reports at Hillside National Wildlife Refuge for 2011 and 2012

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — These harvest forms are from Hillside during the 2011-2012 deer season. They measure weight, antler size, prevalence of milk, and number of deer harvested.

  12. Mathews Brake National Wildlife Refuge Deer Harvest Records from 2011 Season

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — These harvest data are from Mathews Brake during the 2011 deer season. They measure weight, antler size, prevalence of milk, and number of deer harvested.

  13. Morgan Brake National Wildlife Refuge Deer Harvest Records from 2011 Season

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — These harvest data are from Morgan Brake during the 2011 deer season. They measure weight, antler size, prevalence of milk, and number of deer harvested.

  14. Deer Velvet

    Science.gov (United States)

    Deer velvet covers the growing bone and cartilage that develops into deer antlers. People use deer velvet as medicine for a wide range of health problems. Deer velvet is used to boost strength and endurance, ...

  15. Structure of the Paleozoic rocks in the Tonkin Summit Quadrangle, Eureka County, Nevda

    Science.gov (United States)

    Arney, Eric

    Paleozoic rocks in the northern Simpson Park Range, Tonkin Summit Quadrangle, are comprised of the syn-orogenic Roberts Mountains allochthon, the postorogenic Permian Garden Valley Formation, and autochthonous Devonian carbonates. Complex deformation includes the Late Devonian-Early Mississippian, Antler Orogeny, post-Antler thrusting, and Cenozoic Basin and Range extension. The Roberts Mountains thrust caused eastward advancement of deep marine, mainly siliciclastic strata on top of the shelfal, mainly carbonate platform during the Antler Orogeny. This study shows that an east-vergent, post-Antler thrust, emplace the topographically higher carbonate outliers of the autochthon on top of the Roberts Mountains allochthon. These carbonate masses sit on top of the Henderson thrust in the Tonkin Summit Quadrangle and timing of this thrust is constrained to be post-Permian.

  16. Deer Management Assistant Reports at Hillside National Wildlife Refuge for 2012 and 2013

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — These checklists are produced by the Mississippi Department of Wildlife, Fisheries, and Parks and measure harvest weights and antler indexes as well as recommending...

  17. Deer Management Assistance Reports Mathews Brake National Wildlife Refuge for 2012 and 2013

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — These checklists are produced by the Mississippi Department of Wildlife, Fisheries, and Parks and measure harvest weights and antler indexes as well as recommending...

  18. From Delirium to Coherence: Shamanism and Medicine Plants in Silko's "Ceremony"

    Science.gov (United States)

    Weso, Thomas F.

    2004-01-01

    A nondescript rock shelter in Texas provides the evidence for shamanism in Leslie Marmon Silko's novel, "Ceremony". There, archaeologists found identifiable images of antlered human figures and entheogenic plant substances, which are medicinal plants, associated with shamanistic practices.

  19. Ornament Complexity Is Correlated with Sexual Selection: (A Comment on Raia et al., "Cope's Rule and the Universal Scaling Law of Ornament Complexity").

    Science.gov (United States)

    Holman, Luke; Bro-Jørgensen, Jakob

    2016-08-01

    Raia et al. propose that the evolution of the shape and complexity of animal ornaments (e.g., deer antlers) can be explained by interspecific variation in body size and is not influenced by sexual selection. They claim to show that ornament complexity is related to body size by an 0.25-power law and argue that this finding precludes a role for sexual selection in the evolution of ornament complexity. However, their study does not test alternative hypotheses and mismeasures antler shape allometry by omitting much of the published data. We show that an index of sexual selection (sexual size dimorphism) is positively correlated with size-corrected antler complexity and that the allometric slope of complexity is substantially greater than 0.25, contra Raia et al. We conclude that sexual selection and physical constraints both affect the evolution of antler shape. PMID:27420791

  20. Like a fish out of water

    DEFF Research Database (Denmark)

    Philippsen, Bente

    Terrestrial herbivores are usually considered "safe" for radiocarbon dating, as they incorporate carbon in equilibrium with the atmosphere. Radiocarbon reservoir effects are therefore not expected in these animals. One species, however, might show reservoir effects: the elk/moose (Alces alces...... of their time cooling down in lakes and rivers, munching away on the aquatic vegetation. This is the season when the antlers are formed, so the reservoir effect is expected to be greatest in the antlers. In this study, radiocarbon dating of historical known-age samples, archaeological elk bones from secure...... contexts, and paired samples of elk and terrestrial samples, or of elk bones and antlers, will show how great the risk of reservoir effects in elk is. I aim at a great temporal and geographical variety of sample origins, with a special focus on areas with carbonate-rich water, where the largest effects...

  1. Dama roberti, a new species of deer from the early Middle Pleistocene of Europe, and the origins of modern fallow deer

    Science.gov (United States)

    Breda, Marzia; Lister, Adrian M.

    2013-06-01

    The ancestry of the modern fallow deer, Dama dama, has been tentatively traced back to Pliocene/Early Pleistocene forms referred to 'Pseudodama', characterized by unpalmated three- or four-point antlers. By the late Middle Pleistocene, Dama with palmated antlers appears, as Dama dama clactoniana. However, fallow deer from the interim period, the early Middle Pleistocene, are poorly-known. A new specimen from Pakefield (Suffolk, UK), represented by a portion of cranium with a substantial part of both antlers plus a mandible and scapula, is the most complete medium-sized deer specimen from the British early Middle Pleistocene (ca 700 ka). The position and orientation of the basal tine, together with dental characters and mandibular morphology, are typical of fallow deer. The narrow palmation is reminiscent of D. dama clactoniana, but the lack of palmation tines is unique. Moreover, the lack of second (and third) tines in an adult specimen differs from both D. dama dama and D. d. clactoniana, being a primitive character shared with the last representatives of 'Pseudodama' which, on the other hand, has a circular beam lacking any palmation. This combination of features justifies the erection of a new species provisionally placed within the genus Dama, Dama roberti n. sp. Another specimen, from Soleilhac (Auvergne, France), represented by portions of the two antlers, a mandible and a tibia, shares antler morphology with the Pakefield specimen and can be ascribed to the same new species. Isolated antler and dental remains from coeval British sites are tentatively ascribed to D. roberti n. sp. The new species has implications for the ancestry of modern fallow deer.

  2. Råmaterialer, forarbejder, spild

    DEFF Research Database (Denmark)

    Roesdahl, Else; Dobat, Andres S.; Pedersen, Unn;

    2014-01-01

    Finds from Aggersborg: raw iron, off-cuts and a ?bar, hammer-scale, slag, rough-outs of arrow-heads and leister-prongs, lead waste, copper-alloy raw material and waste, gold and amber waste, raw material, rough-outs and waste of bone, antler and horn, soapstone, pigment?......Finds from Aggersborg: raw iron, off-cuts and a ?bar, hammer-scale, slag, rough-outs of arrow-heads and leister-prongs, lead waste, copper-alloy raw material and waste, gold and amber waste, raw material, rough-outs and waste of bone, antler and horn, soapstone, pigment?...

  3. 水池边的牡鹿%THE · STAG· AT· THE· POOL

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ A thirsty Stag went to a Pool to drink. As he saw his own reflection(倒影) in the water, he was struck with admiration (钦慕) for his fine antlers(角), but at the same time felt disgust for the weakness and slendemess of his legs.

  4. Geophysical expression of the Ghost Dance Fault, Yucca Mountain, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Ponce, D.A.; Langenheim, V.E. [Geological Survey, Menlo Park, CA (United States)

    1995-12-01

    Gravity and ground magnetic data collected along surveyed traverses across Antler and Live Yucca Ridges, on the eastern flank of Yucca Mountain, Nevada, reveal small-scale faulting associated with the Ghost Dance and possibly other faults. These studies are part of an effort to evaluate faulting in the vicinity of a potential nuclear waste repository at Yucca Mountain.

  5. Geophysical expression of the Ghost Dance Fault, Yucca Mountain, Nevada

    International Nuclear Information System (INIS)

    Gravity and ground magnetic data collected along surveyed traverses across Antler and Live Yucca Ridges, on the eastern flank of Yucca Mountain, Nevada, reveal small-scale faulting associated with the Ghost Dance and possibly other faults. These studies are part of an effort to evaluate faulting in the vicinity of a potential nuclear waste repository at Yucca Mountain

  6. Morphological change in Newfoundland caribou: Effects of abundance and climate

    Directory of Open Access Journals (Sweden)

    Shane P. Mahoney

    2011-04-01

    Full Text Available The demographic and environmental influences on large mammal morphology are central questions in ecology. We investigated the effects of population abundance and climate on body size and number of male antler points for the La Poile and Middle Ridge caribou (Rangifer tarandus, L. 1758 herds, Newfoundland, Canada. Across 40 years and 20-fold changes in abundance, adult males and females exhibited diminished stature as indicated by jawbone size (diastema and total mandible length and the number of antler points at the time of harvest. Associations between jawbone size and population abundance at birth were consistently negative for both herds, both sexes, and all age classes. Large-scale climate patterns, as measured by the North Atlantic Oscillation in the winter prior to birth, were also negatively associated with jawbone size. Declines in male antler size, as measured by the number of antler points, were not well predicted by either abundance or climate, suggesting other factors (e.g., current, rather than latent, foraging conditions may be involved. We conclude that these morphological changes indicate competition for food resources.

  7. 78 FR 45956 - Notice of Inventory Completion: Hamilton County Department of Parks and Recreation, Hamilton...

    Science.gov (United States)

    2013-07-30

    ... this notice are the sole responsibility of the museum, institution, or Federal agency that has control... funerary objects are 4 lots of animal bone (burned and unburned); 1 animal incisor tool; 1 antler... humpback knife; 1 lot of intermediate bone tool; 4 lots of light and heavy fraction; 1 modified...

  8. Seroprevalence, isolation, first genetic characterization of Toxoplasma gondii, and possible congenital transmission in wild moose from Minnesota, USA

    Science.gov (United States)

    Toxoplasma gondii infections are widespread in white tailed deer (Odocoileus virginianus) but little is known of its prevalence in other cervids in the USA. Moose (Alces alces) is a popular large game animal, hunted for its meat and trophy antlers. Here, we report seroprevalence, isolation and genet...

  9. PALEOZOOLOGY

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    20151109Deng Tao(Key Laboratory of Vertebrate Evolution and Human Origins,Institute of Vertebrate Paleontology and Paleoanthropology,Chinese Academy of Sciences,Beijing100044,China);Lu Xiaokang A New Species of Crown-Antlered Deer Stephanocemas(Cervidae,Artiodactyla)from the Middle

  10. Deer antler:A stem cell-based organ regeneration research model%基于干细胞的器官再生研究模型-鹿茸

    Institute of Scientific and Technical Information of China (English)

    褚文辉; 王大涛; 鲁晓萍; 李春义

    2013-01-01

    BACKGROUND:Deer antlers are the unique mammalian organs which can periodical y regenerate, and the process is known as a stem cel-based event. Exploring the underlying mechanism of deer antler regeneration and indentifying the functional role of stem cellin mammalian organ regeneration are of great importance to regenerative biology and regenerative medicine. OBJECTIVE:To review the relevant literatures of the research progress in antler regeneration, as wel as effects of stem cells and cytokines on antler regeneration. METHODS:A computer-based online search of PubMed (1994-01/2012-10) was performed for acquiring the articles in English by using the key words of“deer antler;antler regeneration;stem cell. In addition, manual search was also performed for those literatures that cannot be readily obtained from internet search. Articles concerning antler regeneration histology, morphology, antler stem cells and micro-environmental studies, and related cytokines. Repetitive studies or articles that are unrelated to the criteria set for the article were excluded. RESULTS AND CONCLUSION:A total of 87 articles were obtained and final y 31 articles were selected. The key tissue types for antler regeneration are antlerogenic periosteum and pedicle periosteum, the cells within which are known as antler stem cells. The covering skin of antlerogenic periosteum and pedicle periosteum constitutes the functional niche for antler stem cells. Numerous cytokines are involved in the process of antler fast growing and ful regeneration, including insulin-like growth factor, sex hormones, human epidermal growth factor, and vascular endothelial growth factor. It is vital y important to identify the interacting molecules between the antler stem cells and their niche celltypes, and to define the role of each molecule that plays in antler regeneration, which wil greatly advance our knowledge of the stem cel-based mammalian organ regeneration.%背景:鹿茸是惟一能够周期性再生

  11. Current Situation of Venison Processing and Utilization%鹿肉的加工及利用现状

    Institute of Scientific and Technical Information of China (English)

    闫晓侠; 刘芳; 马进喜; 张铁华; 秦凤贤; 胡铁军; 孙尧; 刘晶; 王朝晖; 张凤宽; 尤丽新; 于研

    2014-01-01

    鹿全身都是宝,鹿茸、鹿托盘、鹿血等都是经过研究具有很高的营养价值。目前,鹿肉的利用还不是很多,本文主要介绍了鹿肉的国内外发展现状、鹿肉的相关产品、鹿肉的未来发展前景和对鹿肉未来市场的展望。%In China, deer is an important economic animal. No part of deer goes to waste; the antler, antler base and blood have been demonstrated to have high nutritional value. However, to date venison has been utilized scarcely. This review focuses on the current situation of deer meat processing and related products as well as future development and market prospects.

  12. From flakes to grooves: a technical shift in antlerworking during the last glacial maximum in southwest France.

    Science.gov (United States)

    Pétillon, Jean-Marc; Ducasse, Sylvain

    2012-04-01

    The evolution of antlerworking technology in Paleolithic and Mesolithic Europe, especially the production of splinters, is usually described as a cumulative process. A progressive increase in blank standardization and productivity was prompted by the application of a key technical process, the groove and splinter technique (GST). The Badegoulian, however, appears as an interruption in this continuum. According to the original definition of this post-Solutrean, pre-Magdalenian archeological culture, one of its distinctive features is the absence of the GST and the manufacture of antler blanks by knapping only. However, this conception has been recently questioned, leading to an alternative hypothesis suggesting that both GST and knapping were used during the Badegoulian. In this article, we present new evidence from several sites in southwest France, which sheds new light on the issue of Badegoulian antlerworking and the transition with the subsequent Lower Magdalenian. Our study is based on two complementary methods: the technological analysis of antler assemblages well-dated to the Badegoulian (Le Cuzoul de Vers) or to the Lower Magdalenian (La Grotte des Scilles, Saint-Germain-la-Rivière), and the direct (14)C dating of specific antler artifacts from mixed or problematic contexts (Cap-Blanc, Reverdit and Lassac). The results firmly establish that, in southwest France, knapping is the only method used for the production of antler splinters during the Badegoulian, before ca. 20,500 cal BP (calibrated years before present), and that it is rapidly replaced by the GST at the beginning of the Lower Magdalenian, after ca. 20,500 cal BP. This technical shift is not linked to an influx of new human populations, environmental change or the supposed economic advantages of the GST. Instead, it must be understood as one of the expressions of a broader reconfiguration of the technical world that starts to take shape in the middle of the Last Glacial Maximum. PMID:22386151

  13. Bionics: Biological insight into mechanical design

    OpenAIRE

    Dickinson, Michael H

    1999-01-01

    When pressed with an engineering problem, humans often draw guidance and inspiration from the natural world (1). Through the process of evolution, organisms have experimented with form and function for at least 3 billion years before the first human manipulations of stone, bone, and antler. Although we cannot know for sure the extent to which biological models inspired our early ancestors, more recent examples of biomimetic designs are well documented. For example, birds and bats played a cen...

  14. Selection of trees for rubbing by red and roe deer in forest plantations

    OpenAIRE

    Ramos, Jaime; Bugalho, Miguel; Cortez, José Paulo; Iason, Glenn

    2006-01-01

    Antler rubbing is a form of behaviour by which deer may damage and ultimately induce mortality of trees. Understanding factors affecting selection of trees for rubbing may contribute to mitigation of negative effects of such behaviour in plantations or woodlands. We analysed characteristics of trees rubbed by red and roe deer along transects established in plantations of Pinus pinaster (Aiton), Pseudotsuga menziesii (Mirbel) Franco, Betula alba L. and Quercus robur L. in Northeast Portugal. T...

  15. Talking about Dragon in the Dragon Year

    Institute of Scientific and Technical Information of China (English)

    Liu Zhiyang

    2012-01-01

    In the five-thousand-year history of Chinese nation,dragon is the embodiment of Chinese culture,It represents the profound cultural deposits and expresses the cultural features of China.The characteristics of dragon are snake's body,eagle's claws,deer's antlers and tiger's teeth-the most featured and powerful pans from various animals.It indicates that Chinese culture absorbs merits of other cultures and integrates them,which is also the distinctive feature of Chinese culture.

  16. Wuji Baifeng Wan White Phoenix Bolus of Black-Bone Chicken

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    *Source It is a modified recipe from "Shoushi Baoyuan" (Preserving Essence to Extending Life-span) by Gong Tingxian of Ming Dynasty in beginning of 17th century, the imperial hospital listed as specific drug for royal palace. Carried in Pharmacopoeia of P.R.China (1995 Edition)  *Chief Ingredients Black-bone chicken, Antler glue, Turtle shell, Oyster shell, Mantis egg-case, Ginseng root, Milkvetch root, Chinese angelica root, White peony root, Nutgrass flatsedge rhizome, Lucid asparagus root, Licorice root, Rehmannia root, Prepared rehmannia root, Chuanxiong rhizome, Stellaria root, Red sage root, Chinese yam, Gordon euryale seed, Deglued antler powder.  *Explanation The black-bone chicken can replenish Liver and Kidney, Qi and blood, serve as principal drug; Antler glue, Mantis warm Kidney Yang while Turtle and Oyster shell, Asparagus, Stellaria nourish Yin to clear asthenia heat, Ginseng, Milkvetch, Yam, Licorice, Euryale tonify Spleen Qi, and Chinese angelica, Chuanxiong, Peony, Rehmannia, Red sage replenish blood and regulate menstruation.  *Function Replenishing Qi and nourishing blood, regulating menstruation and arresting vaginal discharge  *Indication Deficiency of both Qi and blood, pathological wasting and asthenia, aching and weak loins and knees, irregular menstruation, metrorrhagia metrostaxis, leukorrhagia……

  17. Wuji Baifeng Wan White Phoenix Bolus of Black-Bone Chicken 乌鸡白凤丸

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@*Source It is a modified recipe from "Shoushi Baoyuan" (Preserving Essence to Extending Life-span) by Gong Tingxian of Ming Dynasty in beginning of 17th century, the imperial hospital listed as specific drug for royal palace. Carried in Pharmacopoeia of P.R.China (1995 Edition) *Chief Ingredients Black-bone chicken, Antler glue, Turtle shell, Oyster shell, Mantis egg-case, Ginseng root, Milkvetch root, Chinese angelica root, White peony root, Nutgrass flatsedge rhizome, Lucid asparagus root, Licorice root, Rehmannia root, Prepared rehmannia root, Chuanxiong rhizome, Stellaria root, Red sage root, Chinese yam, Gordon euryale seed, Deglued antler powder. *Explanation The black-bone chicken can replenish Liver and Kidney, Qi and blood, serve as principal drug; Antler glue, Mantis warm Kidney Yang while Turtle and Oyster shell, Asparagus, Stellaria nourish Yin to clear asthenia heat, Ginseng, Milkvetch, Yam, Licorice, Euryale tonify Spleen Qi, and Chinese angelica, Chuanxiong, Peony, Rehmannia, Red sage replenish blood and regulate menstruation. *Function Replenishing Qi and nourishing blood, regulating menstruation and arresting vaginal discharge *Indication Deficiency of both Qi and blood, pathological wasting and asthenia, aching and weak loins and knees, irregular menstruation, metrorrhagia metrostaxis, leukorrhagia

  18. Investigation of anatomical anomalies in Hanford Site mule deer

    International Nuclear Information System (INIS)

    Rocky Mountain mule deer (Odocoileus hemionus hemionus), common residents of the Hanford Site, are an important part of the shrub-steppe ecosystem as well as being valued for aesthetics and hunting. Because mule deer have been protected from hunting on the Site for 50 years, the herd has developed unique population characteristics, including a large number of old animals and males with either large or atypically developed antlers, in contrast to other herds in the semi-arid regions of the Northwest. Hanford Site mule deer have been studied since 1991 because of the herd's unique nature and high degree of public interest. A special study of the mule deer herd was initiated in 1993 after observations were made of a relatively large number of male deer with atypical, velvet-covered antlers. This report specifically describes our analyses of adult male deer found on the Site with atypical antlers. The report includes estimates of population densities and composition; home ranges, habitat uses, and dietary habits; natural and human-induced causes of mortality; and the herd's overall health and reproductive status

  19. Selection decisions among reindeer herders in Finland

    Directory of Open Access Journals (Sweden)

    Kirsi Muuttoranta

    2011-04-01

    Full Text Available Selection of breeding animals is a tool to improve the revenues in animal production. Information about selection practices and criteria are essential in assessing the possibilities for systematic selection schemes. Attitudes of reindeer herders towards use of selection in improving production were investigated by means of interviews. We interviewed the managers of reindeer herding cooperatives concerning their selection decisions. Fortyfive out of 56 managers answered to the semi-structured questionnaire. Among herding operations, selection of breeding animals was regarded by managers as critical for calf’s autumn weight and survival. The main selection criteria were calf’s health, vigour, body size and muscularity, dam or dam line, and maternal care. Hair quality and hair length were important as well, while such often quoted traits as antler characteristics, e.g. early shedding of antler velvet and thick antler bases, were unimportant. The results show that reindeer herders i acknowledge the importance and effects of selective breeding, and ii have empirical knowledge to list the most important selection criteria.

  20. Preparation of oriented linear copper fiber sintered felt and its performance

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Long metal fibers were manufactured in horizontal lathe with a multi-tooth tool. Based on the coarse antler surface structure of copper fibers, a new sintering technology was put forward to manufacture a kind of oriented linear copper fiber sintered felt. The sintering mechanism of oriented linear copper fiber sintered felt was studied. Compared with sintered copper-wire felt, the characteristics of sintered copper-fiber felts were analyzed in details. Owing to the coarse antler surface structure of copper fibers,oriented linear copper-fiber felt was sintered under the condition of micro/nano scale range, and copper fibers easily bonded together in the sintering process. Microchannels with micro-scale coarse antler surface structure were constructed. These characters give oriented linear copper fiber felt some new merits: high filtration accuracy, high flow capability, low resistance loss, good capability to resistance pressure, stable and uniform pore, high specific surface area. The properties of oriented linear copper fiber sintered felt were analyzed.

  1. Investigation of anatomical anomalies in Hanford Site mule deer

    Energy Technology Data Exchange (ETDEWEB)

    Tiller, B.L.; Cadwell, L.L.; Poston, T.M. [and others

    1997-03-01

    Rocky Mountain mule deer (Odocoileus hemionus hemionus), common residents of the Hanford Site, are an important part of the shrub-steppe ecosystem as well as being valued for aesthetics and hunting. Because mule deer have been protected from hunting on the Site for 50 years, the herd has developed unique population characteristics, including a large number of old animals and males with either large or atypically developed antlers, in contrast to other herds in the semi-arid regions of the Northwest. Hanford Site mule deer have been studied since 1991 because of the herd`s unique nature and high degree of public interest. A special study of the mule deer herd was initiated in 1993 after observations were made of a relatively large number of male deer with atypical, velvet-covered antlers. This report specifically describes our analyses of adult male deer found on the Site with atypical antlers. The report includes estimates of population densities and composition; home ranges, habitat uses, and dietary habits; natural and human-induced causes of mortality; and the herd`s overall health and reproductive status.

  2. Estudo de uma população relictual de veado-campeiro, Ozotoceros bezoarticus (Linnaeus (Artiodactyla, Cervidae no municipio da Lapa, Paraná, Brasil A relictual population study of pampas deer, Ozotoceros bezoarticus (Linnaeus (Artiodactyla, Cervidae at Lapa, Paraná State, Brazil

    Directory of Open Access Journals (Sweden)

    Fernanda Góss Braga Mauro de Moura-Britto

    2000-03-01

    Full Text Available The pampas deer, Ozotoceros bezoarticus (Linnaeus, 1758 was studied between August 1996 and September 1997 at Fazenda Santa Maria (25º34' and 25º36'S, 49º46'and 49º49'W at Lapa, Paraná State. During that period we have collected information on feeding habits, group composition, time of the year for birth of youngsters and time for antlers change. The presence of the animal in the area was confirmed by the finding of vestiges and visual occurrences. Animais were observed feeding on young leaves of soya been (Glicine max, oat (Avenasaliva, ryegrass (Lolium mulliflorum and barley (Hordeum vulgare. Only one female was observed feeding on dry leaves. A stag was observed feeding on a barley ear. Group's average size was 1.5 individual. Stags/female proportion was 1: 0.96. Animais displaying velvet coated antlers were observed in June and July which is in accordance with literature about antlers change in a definite period restricted to winter. Only one youngster was observed in September 1996. It is believed that the species survive in the region despite human activities through adaptation of feeding habits with the introduction of agricultura! species. This population could disappear in a few years because of the small number of individuais and their isolation.

  3. SUPPORT MODEL FOR BREEDING THE RED DEER (CERVUS ELAPHUS, L. IN THE OPEN HUNTING GROUNDS

    Directory of Open Access Journals (Sweden)

    Dražen Degmečić

    2009-12-01

    Full Text Available The aim of this paper is to establish support systems which would resolve the supervision and control of the management plan, ensure sustainability of quality products and create conditions for stability of income. The study was carried out over five hunting seasons (2004/2005 to 2008/2009. Research area was hunting ground „Podunavlje - Podravlje XIV / 9," lowland type, altitude 65 to 120 m, situated between the rivers Danube and Drava in Baranja. The total area within the boundaries of the hunting ground is 26,810 ha. Field data are classified into the following age and sex classes: calves, yearling females, hinds, yearling males, males 3, 4 and 5 years old, males 6, 7 and 8 years old, and males 9 years old and older. The observed parameters were: the net body weight, fertilization, fetus length and weight, the length of the antler branches, the length of the third tine, the number of tines, weight and value of the antlers in the CIC. Statistical data processing and distribution of values of the parameters were established for each age and sex class. The values of parameters are the selection standards that should be reached by deer of every age and gender, or standards within which the values of certain parameters should be in order to enable support with which, together with selection shooting, clear standards can be set for the observed population. Parameter selection for calves is body weight. The accuracy of selection is monitored according to net body weight. Arithmetic mean of net body weight of calves is 37.77 kg. Yearling females were selected on estimates of body mass. A kind of threshold net body weight was established that is required for mating and successful fertilization. Net body weight that yearling females must achieve in order to be fertilized is 55 kg to 60 kg. Arithmetic mean of net body mass for yearling females is 53.13 kg, and the fertilization rate 47%. For hinds it is essential to maintain body weight around the mean

  4. Evolution of Devonian carbonate-shelf margin, Nevada

    Science.gov (United States)

    Morrow, J.R.; Sandberg, C.A.

    2008-01-01

    The north-trending, 550-km-long Nevada segment of the Devonian carbonate-shelf margin, which fringed western North America, evidences the complex interaction of paleotectonics, eustasy, biotic changes, and bolide impact-related influences. Margin reconstruction is complicated by mid-Paleozoic to Paleogene compressional tectonics and younger extensional and strike-slip faulting. Reports published during the past three decades identify 12 important events that influenced development of shelf-margin settings; in chronological order, these are: (1) Early Devonian inheritance of Silurian stable shelf inargin, (2) formation of Early to early Middle 'Devonian shelf-margin basins, (3) propradation of later Middle Devonian shelf margin, (4) late Middle Devonian Taghanic ondap and continuing long-term Frasnian transgression, (5) initiation of latest Middle Devonian to early Frasnian proto-Antler orogenic forebulge, (6) mid-Frasnian Alamo Impact, (7) accelerated development of proto-Antler forebulge and backbulge Pilot basin, (8) global late Frasnian sentichatovae sea-level rise, (9) end-Frasnian sea-level fluctuations and ensuing mass extinction, (10) long-term Famennian regression and continept-wide erosion, (11) late Famennian emergence: of Ahtler orogenic highlands, and (12) end-Devonian eustatic sea-level fall. Although of considerable value for understanding facies relationships and geometries, existing standard carbonate platform-margin models developed for passive settings else-where do not adequately describe the diverse depositional and, structural settings along the Nevada Devonian platform margin. Recent structural and geochemical studies suggest that the Early to Middle Devonian-shelf-margin basins may have been fault-bound and controlled by inherited Precambrian structure. Subsequently, the migrating latest Middle to Late Devonian Antler orogenic forebulge exerted a dominant control on shelf-margin position, morphology, and sedimentation. ??Geological Society of

  5. Nytt om runer, Nr. 7 (1992)

    OpenAIRE

    1992-01-01

    Nytt om runer, sjuende årgang, s. 3. Arbejdet ved Runologisk-epigrafisk Laboratorium, København, s. 3. Die Runenarbeit am Seminar für Deutsche Philologie (Arbeitsstelle: Germanische Altertumskunde), Göttingen, s. 5. New runic fragment from the Isle of Man, s. 6. German runes in Kent?, s. 7. Comment on the Boarley inscription, Kent, s. 8. Comment on the deer's antler from Brandon, Suffolk, s. 8. Runen aus neuerer Zeit, s. 9. Arbeidet ved Runearkivet, Oslo, s. 10. ...

  6. Elements of the Yue Culture in Jade Ornaments in the Ancient Yun- nan-Guizhou Plateau%云贵高原古代玉饰的越文化因素

    Institute of Scientific and Technical Information of China (English)

    杨建芳

    2004-01-01

    In remote antiquity, members of the Yue people in the region south of the Five Ridges migrated westward by the water route of Xijiang-Yujiang-Yongjiang-Youjiang and settled in the Yunnan-Guizhou Plateau. This led to the wide spread of the jades originally prevailing in the territory ofthe Yue people, such as "horned", crescent and square jue penannular ornaments, necked bracelets and mushroom-shaped fittings for sword butt-ends. With the development of amalgamation of ethnic groups, these types of jades (along with a few antler artifacts) became ornaments of the natives in the Yunnan-Guizhou Plateau, including the Yelang, Dian, Qiongdu and Quting groups.

  7. 黑龙江海林市细林河遗址出土的动物骨骼遗存研究%A Study of the Animal Remains from the Xilinhe Site in Hailin City, Heilongjiang

    Institute of Scientific and Technical Information of China (English)

    陈全家; 张伟; 王培新

    2004-01-01

    In 1996, a batch of animal remains was unearthed from the Xilinhe site in Hailin City, Heilongjiang Province. It falls into two categories: domesticated and wild. The former includes horse's, cattle's, dog's and pig's bones; the latter, brown bear's, wolf's, horse's, deer's and roe deer's remains. In the present paper, the authors report their analysis of the traces on the surface of animal bones, the making techniques of the bone and antler objects, the proportion of animal husbandry to hunting reflected from the site, and the then natural-ecological settings. These researches resulted in the achievement of a great amount of important information on man's action and other fields.

  8. Danielle Bassett: Opening Up the Brain with Network Science.

    Science.gov (United States)

    Campbell, Sarah

    2016-01-01

    Here's the drill. Upon waking, count down from 100 to one as quickly as possible. Next, recite the alphabet, giving each letter a corresponding word partner (A, antler; B, bargain; C, cartoon, for example). After that, crank out several lists, numbering each of the items: 20 men's names, 20 women's names, 20 food items, 20 words beginning with the same letter. When you're done, close your eyes, count to 20, and open them. Your mind, according to Mental Aerobics: Exercises for a Stronger, Healthier Mind [1], is now warmed up and ready to face the day. PMID:27414627

  9. The ontogenetic osteohistology of Tenontosaurus tilletti.

    Directory of Open Access Journals (Sweden)

    Sarah Werning

    Full Text Available Tenontosaurus tilletti is an ornithopod dinosaur known from the Early Cretaceous (Aptian-Albian Cloverly and Antlers formations of the Western United States. It is represented by a large number of specimens spanning a number of ontogenetic stages, and these specimens have been collected across a wide geographic range (from central Montana to southern Oklahoma. Here I describe the long bone histology of T. tilletti and discuss histological variation at the individual, ontogenetic and geographic levels. The ontogenetic pattern of bone histology in T. tilletti is similar to that of other dinosaurs, reflecting extremely rapid growth early in life, and sustained rapid growth through sub-adult ontogeny. But unlike other iguanodontians, this dinosaur shows an extended multi-year period of slow growth as skeletal maturity approached. Evidence of termination of growth (e.g., an external fundamental system is observed in only the largest individuals, although other histological signals in only slightly smaller specimens suggest a substantial slowing of growth later in life. Histological differences in the amount of remodeling and the number of lines of arrested growth varied among elements within individuals, but bone histology was conservative across sampled individuals of the species, despite known paleoenvironmental differences between the Antlers and Cloverly formations. The bone histology of T. tilletti indicates a much slower growth trajectory than observed for other iguanodontians (e.g., hadrosaurids, suggesting that those taxa reached much larger sizes than Tenontosaurus in a shorter time.

  10. Game species: extinction hidden by census numbers

    Directory of Open Access Journals (Sweden)

    Carranza, J.

    2003-12-01

    Full Text Available Management of game species may involve a risk of alteration of their genetic properties. Local adaptations may be disrupted if artificially selected individuals from farms or those belonging to distant geographical areas are introduced to increase population density or trophy ‘quality’. In Spain, red deer (Cervus elaphus from different European subspecies have been introduced to increase the size of trophies (antlers of local populations. Legislation against these introductions is not effective for various reasons, and once the individuals are in the Iberian peninsula it is virtually impossible to prevent their spreading throughout the whole territory without a genetic tool to distinguish between autochthonous and foreign specimens. We have developed such a genetic test and propose a strategy to dissuade land-owners from importing foreign deer. Since deer are bred mainly for their antlers, our strategy is based on an agreement with the National Trophy Body in Spain which rejects trophies from foreign populations. Rejection decreases the value of the trophy so that it becomes more profitable to produce autochthonous deer. Using such a strategy at some critical step in the production or commercialisation process may be a good model to apply in protecting genetic properties of exploited species.

  11. Experimental xenoimplantation of antlerogenic cells into mandibular bone lesions in rabbits: two-year follow-up.

    Science.gov (United States)

    Cegielski, Marek; Dziewiszek, Wojciech; Zabel, Maciej; Dziegiel, Piotr; Kuryszko, Jan; Izykowska, Ilona; Zatoński, Maciej; Bochnia, Marek

    2010-01-01

    Different types of cells require activation, and take part in annual, dynamic growth of deer antlers. Stem cells play the most important role in this process. This report shows the results of a two-year long observation of xenogenic implant of antlerogenic stem cells (cell line MIC-1). The cells were derived from growing antler of a deer (Cervus elaphus), seeded onto Spongostan and placed in postoperative lesions of mandibular bones of 15 experimental rabbits. The healing process observed in the implantation sites in all rabbits was normal, and no local inflammatory response was ever observed. Histological and immunohistochemical evaluations were performed after 1, 2, 6, 12 and 24 months, and confirmed the participation of xenogenic cells in the regeneration processes, as well as a lack of rejection of the implants. The deficiencies in the bones were replaced by newly formed, thick fibrous bone tissue that underwent mineralization and was later remodelled into lamellar bone. The results of the experiment with rabbits allow us to believe that antlerogenic cells could be used in reconstruction of bone tissues in other species as well.

  12. Effect of hunter selectivity on harvest rates of radio-collared white-tailed deer in Pennsylvania

    Science.gov (United States)

    Buderman, Frances E.; Diefenbach, Duane R.; Rosenberry, C.S.; Wallingford, Bret D.; Long, Eric S.

    2014-01-01

    Radio transmitters are a commonly used tool for monitoring the fates of harvested species, although little research has been devoted to whether a visible radio transmitter changes a hunters' willingness to harvest that animal. We initially surveyed deer hunters to assess their willingness to harvest radio-collared deer and predicted radio collars were unlikely to affect the harvest of antlerless deer, but hunters may be less willing to harvest small-antlered males with radio collars compared to large-antlered males. We fitted white-tailed deer (Odocoileus virginianus) with radio collars that were visible to hunters or with ear-tag transmitters or ear-tags that were difficult to detect visually and estimated if harvest rates differed among marking methods. For females, the best model failed to detect an effect of radio collars on harvest rates. Also, we failed to detect a difference between male deer fitted with radio collars and ear-tag transmitters. When we compared males fitted with radio collars versus ear tags, we found harvest rate patterns were opposite to our predictions, with lower harvest rates for adult males fitted with radio collars and higher harvest rates for yearling males fitted with radio collars. Our study suggests that harvest rate estimates generated from a sample of deer fitted with visible radio collars can be representative of the population of inference. 

  13. A new Euprox from the Late Miocene of Yuanmou, Yunnan Province, China, with interpretation of its paleoenvironment

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The Yuanmou Basin of Yunnan Province is a hotspot for the research of early hominid evolution and its environmental background. During the implementation of the Program "Research of the Origin and Evolution of Early Man and Its Environmental Background" from 1998 to 2001, a huge quantity of hominoid and mammal fossils were collected. The present report systematically describes an Euprox robustus sp. nov. identified during the recent laboratory work on the collected material. Euprox is a group of earliest cervids with true antlers. The new species is the third one of the genus discovered in China. Judged by its morphologic characteristics, the new species feed on juicy and tender leaves of dicotyledon. It implies that the vegetation of its epoch in the Yuanmou Basin is a kind of southern subtropical evergreen forest and the climate is humid and temperate with evident seasonality. The latter is mostly influenced by the monsoon and secondly by the latitude.

  14. Crustal structure in Nevada and southern Idaho from nuclear explosions

    Science.gov (United States)

    Pakiser, L.C.; Hill, D.P.

    1962-01-01

    The time of first arrival of seismic waves generated by 4 underground nuclear explosions at the Nevada Test Site (NTS) and recorded along a line extending north into southern Idaho is expressed as T0 = 0. 00 + Δ/3.0 (assumed), T1 = 0 .40 + Δ/6.03, and T2 = 6.15 + Δ/7.84, where time is in seconds and the shot-detector distance (Δ) is in km. Assuming constant velocities and horizontal layers, crustal thickness in the vicinity of NTS was determined to be 28 km. Delays in the traveltime segment T2, which represents Pn, indicate that the crust may thicken to 32 km in northern Nevada. A third phase, expressed as T3 = 14.48 + Δ/7.84, was also recognized and has arrival times appropriate for SPS. Amplitudes of Pn were determined at 7 places from recordings of seismic waves from one underground nuclear explosion (ANTLER).

  15. An Alternative Interpretation of Plasma Selenium Data from Endangered Patagonian Huemul Deer ( Hippocamelus bisulcus )

    International Nuclear Information System (INIS)

    The prevalence of osteopathology in 57% in the endangered adult Patagonian huemul deer (Hippocamelus bisulcus), malformed antler development, and general lack of recovery were previously suggested to possibly be related to mineral imbalances like selenium (Se) deficiency, and not to stem from fluorosis. From recent bone analyses of these diseased huemul, fluoride levels averaged 58 ppm (SE=10.7), thus eliminating fluorosis as a causal factor for the osteopathology reported in huemul. In contrast, when analyzing high-elevation sites commonly used by extant populations, we found soils deficient in Se. Ashes from recent volcanism also were very low in Se. As Se-responsive diseases in livestock have been documented in Chile, we reclassified recently published Se levels in huemul and determined that 73% were deficient and 18% marginal. Together with these several lines of indirect evidence, we conclude that Se deficiency plays a role in the lack of recovery of huemul populations.

  16. Characterization of multi-layered fish scales (Atractosteus spatula) using nanoindentation, X-ray CT, FTIR, and SEM.

    Science.gov (United States)

    Allison, Paul G; Rodriguez, Rogie I; Moser, Robert D; Williams, Brett A; Poda, Aimee R; Seiter, Jennifer M; Lafferty, Brandon J; Kennedy, Alan J; Chandler, Mei Q

    2014-07-10

    The hierarchical architecture of protective biological materials such as mineralized fish scales, gastropod shells, ram's horn, antlers, and turtle shells provides unique design principles with potentials for guiding the design of protective materials and systems in the future. Understanding the structure-property relationships for these material systems at the microscale and nanoscale where failure initiates is essential. Currently, experimental techniques such as nanoindentation, X-ray CT, and SEM provide researchers with a way to correlate the mechanical behavior with hierarchical microstructures of these material systems1-6. However, a well-defined standard procedure for specimen preparation of mineralized biomaterials is not currently available. In this study, the methods for probing spatially correlated chemical, structural, and mechanical properties of the multilayered scale of A. spatula using nanoindentation, FTIR, SEM, with energy-dispersive X-ray (EDX) microanalysis, and X-ray CT are presented.

  17. Biomimetic hydroxyapatite as a new consolidating agent for archaeological bone

    Science.gov (United States)

    North, Alexis E.

    Recent studies on calcareous stone and plaster consolidation have demonstrated considerable potential by bio-mimicking the growth of hydroxyapatite (HAP), the main mineralogical constituent of teeth and bone matrix. These initial conservation applications, together with significant fundamental research on the precipitation of HAP for bioengineering and biomedical applications, offer great promise in the use of HAP as a consolidating agent for archaeological bone and other similar materials such as archaeological teeth, ivory, and antler. Experimental research via the controlled application of diammonium phosphate (DAP) precursors to bone flour, modern bone samples, and archaeological bones, indicated the in situ formation of HAP with a simultaneous increase in the cohesiveness of friable bone material, while preserving the bone's physiochemical properties. These preliminary results point towards a promising new method in archaeological conservation.

  18. Molybdenum and copper levels in white-tailed deer near uranium mines in Texas

    Science.gov (United States)

    King, K.A.; LeLeux, J.; Mulhern, B.M.

    1984-01-01

    Molybdenum toxicity, molybdenosis, in ruminant animals has been identified in at least 15 states and in Canada, England, Australia, and New Zealand. In most western states, molybdenosis has been associated with strip-mine spoil deposits. Molybdenum toxicity has been diagnosed in cattle pastured near uranium strip-mine spoils in several Texas counties. Recent reports from hunters and the authors' observations indicated that white-tailed deer (Odocoileus virginianus ) that fed near uranium-mine spoil deposits may also have been exposed to high levels of molybdenum. The objectives of this study were to determine if white-tailed deer from a South Texas uranium mining district were accumulating harmful levels of molybdenum and to compare molybdenum and copper levels with antler development in deer from the mined area vs. an unmined control area.

  19. Mesolithic heritage in early Neolithic burial rituals and personal adornments

    Directory of Open Access Journals (Sweden)

    Eva Lenneis

    2007-12-01

    Full Text Available Some burial rituals such as cremation or the use of colorants, especially ochre, have old roots in the preceding Mesolithic and even in the Palaeolithic. The evidence for these old rituals is more dense in central or western Europe than in south east Europe, whence most of the new Neo- lithic ideas came. Among the personal adornments a small amount of snail-shell ornaments, stag tusks, tusks of wild boar and pendants made from antler are of special interest. People wearing these very traditional, old adornments are generally equipped with precious ‘new’ things such as Spondylus, ceramics, adzes etc, and therefore show them as high status people in early Neolithic society.

  20. Two cases of incidental Podostroma cornu-damae poisoning

    Science.gov (United States)

    Kim, Hee Nyung; Do, Han Ho; Seo, Jun Seok; Kim, Hee Young

    2016-01-01

    Podostroma cornu-damae is a rare, deadly fungus. However, it can be easily mistaken for antler Ganoderma lucidum. In this case report, two patients made tea with the fungus and drank it over a 2-week period. Both patients presented with bicytopenia, and one patient had desquamation of the palms and soles. Both were treated with prophylactic antibiotics and granulocyte colony-stimulating factor. One patient was admitted to the intensive care unit and received a platelet transfusion. Both patients were discharged without complications. Podostroma cornu-damae infections caused by intoxication were successfully treated using our treatment strategy, which consisted of prophylactic antibiotics, platelet transfusion, and granulocyte colony-stimulating factor. We believe this report can guide future treatment. PMID:27752639

  1. 湖北沙市周梁玉桥遗址1987年的发掘%1987 Excavation on the Zhouliangyuqiao Site in Shashi, Hubei

    Institute of Scientific and Technical Information of China (English)

    荆州市周梁玉桥遗址博物馆

    2004-01-01

    This site lies in the northeastern part of Shashi District proper in Jingzhou City, Hubei Province. It has been excavated five times. In 1987, excavation was carried out in Area C, covering 104 sq m. The finds include six ash-pits, distinctive pottery, as well as bronzes, oracle shells with strong chronological features, stone implements, bone objects and antler artifacts. The cultural contents of the site feature the pottery composition in that sandy ware constitutes the main body, reddish-brown vessels form the largest proportion and the check patterns occur most frequently. These show that the site represents a Shang cultural complex with the local cultural elements playing the leading role. The excavation provided new data for the periodization of the site.

  2. Dowling-Degos Disease: A Case Report

    Directory of Open Access Journals (Sweden)

    Selma Emre

    2012-06-01

    Full Text Available Dowling-Degos Disease (DDD, is a rare, autosomal dominantly inherited pigmentation disorder. It is characterized by slowly progressive mottled or reticulated pigmented macules on the axillae, groins, perineum, inframammary regions and other flexural areas. The disease appears after puberty, most frequently during the fourth decade of life. Herein, we report a 42-year old female patient who presented with dark brown hyperpigmented macules on her groins, perineum and inframammary areas at our outpatient clinic. The histopathological examination showed elongation of rete ridges from epidermis to dermis with a filiform or antler-like pattern. Increased melanin pigmentation on the peak of the rete ridges and melanophages in the papillary dermis were seen. Depending on these clinical and histopathological findings, DDD was diagnosed. DDD should be considered in the differential diagnosis of disorders with flexural pigmentation.

  3. Dowling-Degos Disease: A Case Report

    Directory of Open Access Journals (Sweden)

    Selma Emre

    2012-06-01

    Full Text Available Dowling-Degos Disease (DDD, is a rare, autosomal dominantly inherited pigmentation disorder. It is characterized by slowly progressive mottled or reticulated pigmented macules on the axillae, groins, perineum, inframammary regions and other flexural areas. The disease appears after puberty, most frequently during the fourth decade of life. Herein, we report a 42-year old female patient who presented with dark brown hyperpigmented macules on her groins, perineum and inframammary areas at our outpatient clinic. The histopathological examination showed elongation of rete ridges from epidermis to dermis with a filiform or antler-like pattern. Increased melanin pigmentation on the peak of the rete ridges and melanophages in the papillary dermis were seen. Depending on these clinical and histopathological findings, DDD was diagnosed. DDD should be considered in the differential diagnosis of disorders with flexural pigmentation. (Turk J Dermatol 2012; 6: 55-7

  4. An Alternative Interpretation of Plasma Selenium Data from Endangered Patagonian Huemul Deer ( Hippocamelus bisulcus )

    Energy Technology Data Exchange (ETDEWEB)

    Flueck, Werner T. [National Council of Scientific and Technological Research (CONICET), Bariloche (Argentina); Univ. of Basel (Switzerland). Swiss Tropical and Public Health Inst.; ETH Zurich (Switzerland). Inst. of Biogeochemistry and Pollutant Dynamics; Smith-Flueck, Jo Anne M. [Univ. Atlantida Argentina, Bariloche (Argentina). Inst. of Natural Resources Analysis; Mincher, Bruce J. [Idaho National Lab. (INL), Idaho Falls, ID (United States). Aqueous Separations and Radiochemistry Dept.; Winkel, Lenny H. H.E. [Swiss Federal Inst. of Aquatic Science and Technology, Dubendorf (Switzerland). Eawag; ETH Zurich (Switzerland). Inst. of Biogeochemistry and Pollutant Dynamics

    2014-10-01

    The prevalence of osteopathology in 57% in the endangered adult Patagonian huemul deer (Hippocamelus bisulcus), malformed antler development, and general lack of recovery were previously suggested to possibly be related to mineral imbalances like selenium (Se) deficiency, and not to stem from fluorosis. From recent bone analyses of these diseased huemul, fluoride levels averaged 58 ppm (SE=10.7), thus eliminating fluorosis as a causal factor for the osteopathology reported in huemul. In contrast, when analyzing high-elevation sites commonly used by extant populations, we found soils deficient in Se. Ashes from recent volcanism also were very low in Se. As Se-responsive diseases in livestock have been documented in Chile, we reclassified recently published Se levels in huemul and determined that 73% were deficient and 18% marginal. Together with these several lines of indirect evidence, we conclude that Se deficiency plays a role in the lack of recovery of huemul populations.

  5. Reproductive biology of the pampas deer (Ozotoceros bezoarticus: a review

    Directory of Open Access Journals (Sweden)

    Olazabal Daniel

    2008-06-01

    Full Text Available Abstract The pampas deer (Ozotoceros bezoarticus is a South American grazing deer which is in extreme danger of extinction. Very little is known about the biology of the pampas deer. Moreover, most information has not been published in peer-reviewed scientific journals, and is only available in local publications, theses, etc. Therefore, our aim was to update and summarize the available information regarding the reproductive biology of the pampas deer. Moreover, in most sections, we have also included new, unpublished information. Detailed descriptions are provided of the anatomy of both the female and the male reproductive tract, puberty onset, the oestrous cycle and gestational length. Birthing and the early postpartum period are described, as are maternal behaviour and early fawn development, seasonal distribution of births, seasonal changes in male reproduction and antler cycle, reproductive behaviour, semen collection, and cryopreservation. Finally, an overview is given and future directions of research are proposed.

  6. Sobre los llamados silbatos celtibéricos. Una propuesta de interpretación

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    Escudero Navarro, Zoa

    1990-12-01

    Full Text Available In this study we propose a new interpretation for those objects made of deer antler, wich traditionally have been identified as whistles or musical instruments. Starting from other similar European examples, we think that the pieces from the Iberian Peninsula correspond with the cheekpieces of horsebits, psalia, and at the present time must be surely dated to the Second lron Age.

    En el presente artículo proponemos una nueva interpretación para los objetos realizados en asta de ciervo que tradicionalmente se han identificado con silbatos o instrumentos musicales. A partir de otros elementos europeos semejantes, creemos que las piezas de la Península Ibérica se corresponden con camas de bocado de caballo, psalia, y que, hoy por hoy, deben fecharse claramente en la Segunda Edad del Hierro.

  7. Unusual raptor nests around the world

    Science.gov (United States)

    Ellis, D.H.; Craig, T.; Craig, E.; Postupalsky, S.; LaRue, C.T.; Nelson, R.W.; Anderson, D.W.; Henny, C.J.; Watson, J.; Millsap, B.A.; Dawson, J.W.; Cole, K.L.; Martin, E.M.; Margalida, A.; Kung, P.

    2009-01-01

    From surveys in many countries, we report raptors using unusual nesting materials (e.g., paper money, rags, metal, antlers, and large bones) and unusual nesting situations. For example, we documented nests of Steppe Eagles Aquila nipalensis and Upland Buzzards Buteo hemilasius on the ground beside well-traveled roads, Saker Falcon Falco cherrug eyries in attics and a cistern, and Osprey Pandion haliaetus nests on the masts of boats and on a suspended automobile. Other records include a Golden Eagle A. chrysaetos nest 7.0 m in height, believed to be the tallest nest ever described, and, for the same species, we report nesting in rudimentary nests. Some nest sites are within a few meters of known predators or competitors. These unusual observations may be important in revealing the plasticity of a species' behavioral repertoire. ?? 2009 The Raptor Research Foundation, Inc.

  8. The petrosal bone and bony labyrinth of early to middle Miocene European deer (Mammalia, Cervidae) reveal their phylogeny.

    Science.gov (United States)

    Mennecart, Bastien; Rössner, Gertrud E; Métais, Grégoire; DeMiguel, Daniel; Schulz, Georg; Müller, Bert; Costeur, Loïc

    2016-10-01

    Deer (Cervidae) have a long evolutionary history dating back to the Early Miocene, around 19 million years ago. The best known fossils to document this history belong to European taxa, which all bear cranial appendages more or less similar to today's deer antlers. Despite the good fossil record, relationships of the earliest stem deer and earliest crown deer are much debated. This hampers precise calibration against the independent evidence of the fossil record in molecular clock analyses. While much has been written on the Early and Middle Miocene deer, only two phylogenetic analyses have been performed on these taxa to date mostly based on cranial appendage characters. Because the petrosal bone and bony labyrinth have been shown to be relevant for phylogeny in ruminants, we describe for the first time these elements for four iconic early cervids from Europe (Procervulus dichotomus, Heteroprox larteti, Dicrocerus elegans and Euprox furcatus) and include them in a phylogenetic analysis based on the ear region exclusively. The analysis recovered E. furcatus in a sister position to the living red deer (Cervus elaphus). Further, it placed D. elegans in a sister position to Euprox + Cervus and a clade Procervulinae that includes P. dichotomus and H. larteti, in sister position to all other deer. The inclusion of E. furcatus in crown Cervidae, which was previously suggested based on antler morphology, cannot be ruled out here but needs a more comprehensive comparison to other crown deer to be confirmed. J. Morphol. 277:1329-1338, 2016. © 2016 Wiley Periodicals, Inc. PMID:27460747

  9. The first complete skeleton of Megaloceros verticornis (Dawkins, 1868 Cervidae, Mammalia, from Bilshausen (Lower Saxony, Germany: description and phylogenetic implications

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    T. Pfeiffer

    2002-01-01

    Full Text Available The first well preserved, articulated skeleton of a young male deer of Megaloceros verticornis (Dawkins, 1868 was excavated from early Middle Pleistocene sediments of the clay pit of Bilshausen (Unter-Eichsfeld, Lower Saxony. This find made it possible, for the first time, to establish, using cladistic techniques, the systematic position of Megaloceros verticornis among Pleistocene and Holocene plesiometacarpal and telemetacarpal cervids. By contrast to the antler and tooth characters, the postcranial characters, in particular, are suitable for phylogeny reconstruction. Megaloceros verticornis from Bilshausen shows great similarity with M. giganteus of the Upper Pleistocene of Europe in its skeletal morphology, and bootstrap values (BP = 100 show strong support for the monophyly of M. giganteus and M. verticornis. The analysis yields no evidence, however, of a close relationship between Dama and Megaloceros, which has been widely discussed in the literature because of the presence of large, palmated antlers in both genera. Aus der Tongrube von Bilshausen (Unter-Eichsfeld, Niedersachsen konnte das erste, vollständige Skelett eines jugen Hirsches von Megaloceros verticornis (Dawkins, 1868 aus mittelpleistozänen Sedimentablagerungen geborgen werden. Dieser Fund ermöglichte es erstmalig, die systematische Stellung von Megaloceros verticornis im System plesiometacarpaler und telemetacarpaler Hirsche des Pleistozäns und Holozäns auf breiter Basis zu untersuchen. Im Gegensatz zu den Geweih- und Zahnmerkmalen eignen sich die postcranialen Merkmale des Skelettes besonders gut für eine phylogenetische Rekonstruktion der Hirsche. Die Gemeinsamkeit Großer Schaufelgeweihe bei Dama dama und dem Riesenhirsch Megaloceros giganteus hat dazu geführt, beide in eine enge phylogenetische Beziehung zu setzen, was in der Literatur zu einer anhaltenden Kontroverse geführt hat. Die Analyse der Morphologie der postcranialen Elemente zeigt jedoch, dass es keine

  10. Mesola red deer: physical characteristics, population dynamics and conservation perspectives

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    Stefano Mattioli

    2003-10-01

    Full Text Available Abstract The biometry, demography and genetics of red deer Cervus elaphus of Mesola Wood (NE Italy, are presented and discussed in relation to the conservation of this population. Modest body size, low stature, oversimplified antlers and a low reproductive performance characterise red deer from Mesola Wood. The mitochondrial genome showed a private haplotype, different from other red deer in Italy and central Europe. The uniqueness of this nucleus and its biogeographic importance make a long-term conservation plan particularly urgent. Management measures such as fallow deer reduction, winter feeding and pasture mowing were tested, giving promising results. The physical condition of the animals improved, calf and adult mortality declined, and a few cases of antlers with bez tine or crown were reported in this study after four decades. Riassunto Il Cervo della Mesola: caratteristiche fisiche, dinamica di popolazione e prospettive di conservazione La biometria, la demografia e la genetica del cervo Cervus elaphus del Gran Bosco della Mesola (Italia nord-orientale, vengono presentate e discusse in relazione alla salvaguardia di questa popolazione. Il cervo della Mesola risulta caratterizzato dalle modeste dimensioni corporee, dalla struttura semplificata dei palchi e da un basso rendimento riproduttivo. L'analisi del genoma mitocondriale ha evidenziato un aplotipo privato, diverso da quello degli altri cervi italiani e centroeuropei. L'unicità di questo nucleo e la sua importanza biogeografica rendono particolarmente urgente un piano di conservazione a lungo termine. Sono stati verificati interventi gestionali quali la riduzione numerica dei daini, il foraggiamento invernale e lo sfalcio delle superfici a pascolo, con risultati promettenti. Le condizioni fisiche degli animali sono migliorate, la mortalità tra i piccoli e gli adulti è diminuita, e sono stati registrati alcuni

  11. Habitat, wildlife, and one health: Arcanobacterium pyogenes in Maryland and Upper Eastern Shore white-tailed deer populations

    Directory of Open Access Journals (Sweden)

    Melissa M. Turner

    2013-08-01

    Full Text Available Background: Understanding the distribution of disease in wildlife is key to predicting the impact of emerging zoonotic one health concerns, especially for wildlife species with extensive human and livestock interfaces. The widespread distribution and complex interactions of white-tailed deer (Odocoileus virginianus with humans suggest deer population health and management may have implications beyond stewardship of the animals. The intracranial abscessation suppurative meningitis (IASM disease complex in deer has been linked to Arcanobacterium pyogenes, an under-diagnosed and often misdiagnosed organism considered commensal in domestic livestock but associated with serious disease in numerous species, including humans. Methods: Our study used standard bacterial culture techniques to assess A. pyogenes prevalence among male deer sampled across six physiogeographic regions in Maryland and male and female deer in the Upper Eastern Shore under Traditional Deer Management (TDM and Quality Deer Management (QDM, a management protocol that alters population demographics in favor of older male deer. Samples were collected from antler pedicles for males, the top of the head where pedicles would be if present for females, or the whole dorsal frontal area of the head for neonates. We collected nasal samples from all animals by swabbing the nasopharyngeal membranes. A gram stain and catalase test were conducted, and aerobic bacteria were identified to genus and species when possible. We evaluated the effect of region on whether deer carried A. pyogenes using Pearson's chi-square test with Yates’ continuity correction. For the white-tailed deer management study, we tested whether site, age class and sex predisposed animals to carrying A. pyogenes using binary logistic regression. Results: A. pyogenes was detected on deer in three of the 6 regions studied, and was common in only one region, the Upper Eastern Shore. In the Upper Eastern Shore, 45% and 66% of

  12. The Role of Museums in Recovery From Disaster: The Great East Japan Earthquake and Tsunami

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    Sudo Ken‘ichi

    2014-12-01

    Full Text Available The National Museum of Ethnology (Minpaku was founded in 1974 as an Inter-University Research Institute housing a museum and graduate school. A museum is more than a place to store intangible and tangible heritage. Along with its responsibility for conserving and passing on cultural materials, it also creates new culture. On March 11, 2011, Japan was struck by an earthquake and tsunami of unprecedented proportions. From one month after the disaster, conservation experts from Minpaku participated in the rescue of tangible cultural resources for the period of eight months. At the same time, our disaster response team worked with village residents in damaged localities, assisting their efforts to replace costumes and ornaments for traditional performing arts that had been washed out to sea, or to repair damaged lion heads, to aid in reviving traditional performing arts. We had thought that, in the process of revival and recovery, the re-launch of festivals and traditional performing arts would come later than the construction of the homes and livelihoods of the local people. In one case, Minpaku, based on its research, was able to provide deer antlers for the headdresses needed to revive the deer dance, an intangible cultural heritage of a village in Iwate Prefecture. Village elders worked the antlers we donated, restored the costumes, and within a year were able to produce ten full sets of costumes. Subsequently, the deer dance was performed in village after village to calm the spirits of the dead, ward off evil spirits, and restore the confidence of people afflicted by the disaster. In this way, a traditional performing art contributed to the revival and rebuilding of the affected communities. In another village the repair and restoration of stone lions’ heads and providing aid for refugees from the disaster were further other examples of organized activity carried out in connection with the traditional performing arts. In sum, our experience

  13. Is mate choice copying or aggregation responsible for skewed distributions of females on leks?

    Science.gov (United States)

    McComb, K; Clutton-Brock, T

    1994-01-22

    In several lek-breeding populations of birds and mammals, females arriving on leks tend to join males that already have females in their territories. This might occur either because females have an evolved preference for mating with males that are attractive to other females, or because they join groups of other females to obtain greater safety from predation or dangerous harassment by males. We have previously used controlled experiments to show that oestrous fallow deer females join males with established harems because they are attracted to female groups rather than to the males themselves. Here we demonstrate that the preference for males with females over males without females is specific to oestrous females and weak or absent in anoestrous ones, and that it is not associated with a preference for mating with males that have previously been seen to mate with other females. Furthermore, oestrous females given the choice between males that do not already have females with them show no significant preference for antlered over deantlered males or for older males over younger ones. We conclude that female attraction to other females on the lek is likely to be an adaptation to avoiding harassment in mixed-sex herds. In this situation, a male's ability to maintain the cohesion of his harem may be the principal cause of variation in mating success between males.

  14. Ptychographic X-ray nanotomography quantifies mineral distributions in human dentine

    Science.gov (United States)

    Zanette, I.; Enders, B.; Dierolf, M.; Thibault, P.; Gradl, R.; Diaz, A.; Guizar-Sicairos, M.; Menzel, A.; Pfeiffer, F.; Zaslansky, P.

    2015-03-01

    Bones are bio-composites with biologically tunable mechanical properties, where a polymer matrix of nanofibrillar collagen is reinforced by apatite mineral crystals. Some bones, such as antler, form and change rapidly, while other bone tissues, such as human tooth dentine, develop slowly and maintain constant composition and architecture for entire lifetimes. When studying apatite mineral microarchitecture, mineral distributions or mineralization activity of bone-forming cells, representative samples of tissue are best studied at submicrometre resolution while minimizing sample-preparation damage. Here, we demonstrate the power of ptychographic X-ray tomography to map variations in the mineral content distribution in three dimensions and at the nanometre scale. Using this non-destructive method, we observe nanostructures surrounding hollow tracts that exist in human dentine forming dentinal tubules. We reveal unprecedented quantitative details of the ultrastructure clearly revealing the spatially varying mineralization density. Such information is essential for understanding a variety of natural and therapeutic effects for example in bone tissue healing and ageing.

  15. Genetic variation in domestic reindeer and wild caribou in Alaska

    Science.gov (United States)

    Cronin, M.; Renecker, L.; Pierson, B. J.; Patton, J.C.

    1995-01-01

    Reindeer were introduced into Alaska 100 years ago and have been maintained as semidomestic livestock. They have had contact with wild caribou herds, including deliberate cross-breeding and mixing in the wild. Reindeer have considerable potential as a domestic animal for meat or velvet antler production, and wild caribou are important to subsistence and sport hunters. Our objective was to quantify the genetic relationships of reindeer and caribou in Alaska. We identified allelic variation among five herds of wild caribou and three herds of reindeer with DNA sequencing and restriction enzymes for three loci: a DQA locus of the major histocompatibility complex (Rata-DQA1), k-casein and the D-loop of mitochondrial DNA. These loci are of interest because of their potential influence on domestic animal performance and the fitness of wild populations. There is considerable genetic variation in reindeer and caribou for all three loci, including five, three and six alleles for DQA, k-casein and D-loop respectively. Most alleles occur in both reindeer and caribou, which may be the result of recent common ancestry or genetic introgression in either direction. However, allele frequencies differ considerably between reindeer and caribou, which suggests that gene flow has been limited.

  16. Secondary Excavation of the Koguryo Mountain Castle at ,Shitaizi,Shenyang City,Liaoning%辽宁沈阳市石台子高句丽山城第二次发掘简报

    Institute of Scientific and Technical Information of China (English)

    沈阳市文物考古工作队

    2001-01-01

    The secondary excavation of the Shitaizi mountain castle was carried out on the basis of the first digging. It covered an area of 450 sq m. The recorded remains include the excavated sites of the northwestern, eastern, southern and southwestern city-gates, the reservoir and officers-mustering terrace vestiges known through trial diggings, and the drains discovered at the northwestern and eastern city-gates. Among the unearthed objects are pottery (the urn, jar, dish, basin, fu cauldron, bowl, dou stemmed vessel, vessel cover, spindle whorl and disc), bronzes (the buckle, arrowhead and plaque), irons(the arrowhead, armor plate, nail, paring knife, awl, hook, ring, axe, door-nail, door-axle and its base,and cap-like cover), stone tools (the perforated stone, grindstone, spindle whorl and adze) and bone and antler artifacts. The present excavation indicates that this mountain castle was twice damaged by fire.The city-gate structural members found on the gate-sites, such as iron-cast door-leaves, door-axles and their bases, rivets and loops, are all discovered for the first time at Koguryo mountain castle and provide very valuable material for studying the shape and structure of mountain-castle gates in that period.

  17. 驯鹿及其生物学特性研究%Reindeer and Its Biological Characteristics

    Institute of Scientific and Technical Information of China (English)

    常悦; 张玉; 陈巴特尔; 孙亚红

    2013-01-01

    驯鹿分布于北极圈及其周边地区,耐寒怕燥,处于野生或半驯化状态.本文通过对驯鹿的生态分布,形态特征(包括个体大小、被毛、茸角、鼻、蹄、视力),进食习性,繁殖习性,迁徙活动以及天敌等几方面对驯鹿的生物学特性做出较为全面的介绍,以便为今后的驯鹿保护工作奠定基础,从而制定出针对性更强的保护措施.%The reindeer inhabits in the Arctic Circle and its surrounding areas, which is cold resistant and afraid of dryness and in the wild or semi-domesticated state. The paper comprehensively introduced the biological characteristics of the reindeer through such aspects as its ecological distribution, morphological character (including body size, fur, antler, nose, feet and eyesight), eating habit, breeding habit, migrating activities as well as its natural enemies, so as to lay a foundation for the protection work and therefore developing more effective protective measures.

  18. Lower Permian Dry Mountain trough, eastern Nevada: preliminary basin analysis

    Energy Technology Data Exchange (ETDEWEB)

    Schwarz, D.L.; Snyder, W.S.; Spinosa, C.

    1987-08-01

    The Lower Permian Dry Mountain trough (DMT) is one of several basins that developed during the Late Pennsylvanian to Permian along the western edge of the North American continent. A tectonic mechanism has been suggested for the subsidence of the DMT, possibly due to reactivation of the Antler orogenic belt during the waning stages of Ancestral Rocky Mountain deformation. The DMT records marked subsidence with the appearance during the Artinskian (latest Wolfcampian) of a deeper water facies that consists of thin-bedded silty micrites and micritic mudstones rich in radiolarians and sponge spicules, characterized by a relative abundance of ammonoids, and rarer conodonts and Nereites ichnofacies trace fossils. Taxa recovered from a distinctive concretionary horizon at various locations provide an Artinskian datum on which to palinspastically reconstruct the DMT paleogeography. These taxa include ammonoids: Uraloceras, Medlicottia, Marathonites, Crimites, Metalegoceras, properrinitids; and conodonts: Neogondolella bisselli, Sweetognathus whitei, S. behnkeni, and Diplognathodus stevensi. The western margin facies of the DMT consists of Permian Carbon Ridge/Garden Valley Formations. Here, lowermost black Artinskianage euxinic micrites, considered a potential source rock for petroleum generation, are overlain by base-of-slope carbonate apron deposits, which, in turn, are overlain by base-of-slope carbonate apron deposits, which, in turn, are overlain by a thick, eastwardly prograding conglomerate wedge. Seismic profiles across Diamond Valley indicate a 3.0-4.6-km thick Tertiary sequence above the Paleozoic strata.

  19. Discounted Deer Quality Value as a Criterion for Deer Management Decisions

    Directory of Open Access Journals (Sweden)

    Thomas James Straka

    2011-06-01

    Full Text Available Discounted cash flow analysis is a standard financial tool that considers the time value of money and calculates the present value of a future sum of money. A model was developed using the same concept to estimate discounted deer quality value of a white-tailed deer (Odocoileus virginianus herd based on the value and survival rate(s by sex, age. These values are discounted, using market interest rate as the discount rate and as the measure of the time value. The model was used to determine the optimum age for harvest of antlered bucks in commercial hunting operations. Based on discount rate, value by age and quality, and survival rates, optimum harvest age varied from 1.5 years to 5.5 years. Survival rate (s was fundamental in determination of optimal harvest ages. The model was also used to examine the feasibility of management practices aimed at increasing or improving herd condition. Assuming a discount rate of 10% and increasing values for bucks ranging from 1.5 to 5.5 years of age, the optimum harvest ages produced ranged from 5.5 years when s ≥ 80% and 1.5 years when s < 60%.

  20. Using heterozygosity-fitness correlations to study inbreeding depression in an isolated population of white-tailed deer founded by few individuals.

    Science.gov (United States)

    Brommer, Jon E; Kekkonen, Jaana; Wikström, Mikael

    2015-01-01

    A heterozygosity-fitness correlations (HFCs) may reflect inbreeding depression, but the extent to which they do so is debated. HFCs are particularly likely to occur after demographic disturbances such as population bottleneck or admixture. We here study HFC in an introduced and isolated ungulate population of white-tailed deer Odocoileus virginianus in Finland founded in 1934 by four individuals. A total of 422 ≥ 1-year-old white-tailed deer were collected in the 2012 hunting season in southern Finland and genotyped for 14 microsatellite loci. We find significant identity disequilibrium as estimated by g 2. Heterozygosity was positively associated with size- and age-corrected body mass, but not with jaw size or (in males) antler score. Because of the relatively high identity disequilibrium, heterozygosity of the marker panel explained 51% of variation in inbreeding. Inbreeding explained approximately 4% of the variation in body mass and is thus a minor, although significant source of variation in body mass in this population. The study of HFC is attractive for game- and conservation-oriented wildlife management because it presents an affordable and readily used approach for genetic monitoring that allowing identification of fitness costs associated with genetic substructuring in what may seem like a homogeneous population. PMID:25691963

  1. Population dynamics of reindeer

    Directory of Open Access Journals (Sweden)

    L. M. Baskin

    1990-09-01

    Full Text Available Five types of reindeer populations are distinguished in terms of population dynamics, population density, social structure and migration distance. Differences in the biological rhythms of the populations result in calving occuring 20 days before snow melting in all populations as well as maximal utilization by the deer of young green vegetation in summer. The growth of antlers may serve as a regulatior of biological rhytms. Populations differ in the level of social motivation. Formation of groups of not less than 30-35 animals ensures cooperative protection from insects and management of the group by man. The fidelity to the calving sites, summer ranges and constant migration routes is based on the common orientation reactions of the animals and social attraction. The direction and migration routes are detemined by obligate learning. The dynamics of populations depends on the fertility of 2 and 3 year old females which is determined by feeding conditions in summer and the activity of males during the rut. Migration plays an important role in the population dynamics.

  2. Health status of mule deer and white-tailed deer herds on the Rocky Mountain Arsenal

    Energy Technology Data Exchange (ETDEWEB)

    Creekmore, T.E.; Franson, J.C.; Sileo, L. [National Wildlife Health Research Center, Madison, WI (United States); Griess, J.M.; Roy, R.R. [Rocky Mountain Arsenal, Commerce City, CO (United States); Baker, D.L. [Colorado Division of Wildlife, Ft. Collins, CO (United States)

    1994-12-31

    The Rocky Mountain Arsenal is a fenced, 6,900-ha Superfund site under remediation by the US Army and the Shell Oil Company. A variety of environmental contaminants including organochlorine pesticides, metals, and nerve-gas-production by-products are in the soil or in the water on the site. The authors evaluated the health of 18 radio-collared deer (13 mule deer [Odocoileus hemionus] and 5 white-tailed deer [O. virginianus]) collected by gunshot. Prior to collection, more than 4,000 locations of the 18 deer were plotted during a period of more than 2 years. Blood samples from the euthanized animals were collected for serologic, hematologic, and contaminant evaluations. Necropsies were preformed and tissues collected for histopathologic examinations and environmental contaminants analyses. Results indicate that the physical conditions of the mule deer were fair/good and of the white-tailed deer were good. Antibody prevalence against epizootic hemorrhagic disease serotype 2 was 85% and bovine virus diarrhea 56%. Two mule deer had severe testicular atrophy, and one of these animals also had antler deformities. Three mule deer had alopecia with dermatitis and hyperkeratosis. Results of heavy metal, and organochlorine pesticide analyses from blood and tissue samples and other analyses will be presented.

  3. How sexual selection can drive the evolution of costly sperm ornamentation

    Science.gov (United States)

    Lüpold, Stefan; Manier, Mollie K.; Puniamoorthy, Nalini; Schoff, Christopher; Starmer, William T.; Luepold, Shannon H. Buckley; Belote, John M.; Pitnick, Scott

    2016-05-01

    Post-copulatory sexual selection (PSS), fuelled by female promiscuity, is credited with the rapid evolution of sperm quality traits across diverse taxa. Yet, our understanding of the adaptive significance of sperm ornaments and the cryptic female preferences driving their evolution is extremely limited. Here we review the evolutionary allometry of exaggerated sexual traits (for example, antlers, horns, tail feathers, mandibles and dewlaps), show that the giant sperm of some Drosophila species are possibly the most extreme ornaments in all of nature and demonstrate how their existence challenges theories explaining the intensity of sexual selection, mating-system evolution and the fundamental nature of sex differences. We also combine quantitative genetic analyses of interacting sex-specific traits in D. melanogaster with comparative analyses of the condition dependence of male and female reproductive potential across species with varying ornament size to reveal complex dynamics that may underlie sperm-length evolution. Our results suggest that producing few gigantic sperm evolved by (1) Fisherian runaway selection mediated by genetic correlations between sperm length, the female preference for long sperm and female mating frequency, and (2) longer sperm increasing the indirect benefits to females. Our results also suggest that the developmental integration of sperm quality and quantity renders post-copulatory sexual selection on ejaculates unlikely to treat male–male competition and female choice as discrete processes.

  4. Wild reindeer Rangifer tarandus (L. in Chukotka

    Directory of Open Access Journals (Sweden)

    Felix B. Chernyavskii

    1998-03-01

    Full Text Available We reviewed historical records of the abundance and distribution of wild reindeer {Rangifer tarandus L. in Chukotka and studied reindeer numbers, distribution and behavior from 1983 to 1993. There were large numbers of wild reindeer in Chukotka until the end of the eighteenth century, but during the nineteenth century the population declined probably from intensive harvest after the introduction of firearms by the Cossacks. During the nineteenth century herding of domestic reindeer also increased, and reindeer herders continued to hunt wild reindeer intensively. During the 1950s there were only about 8500 wild reindeer in two separate herds in Chukotka. By the late 1970s the wild reindeer population had increased to about 11 000. Ten years later we estimated 16 534 reindeer, and found only one contiguous population. Presently, the population calves and spends the summer in the Anadyr Uplands and migrates west and southwest to spend the winter in forest tundra and northern taiga regions. Predators, primarily wolves and brown bears, kill a significant number of calves. Today, the wild reindeer in Chukotka coexist with 300 000 domestic reindeer. However, current costs of gasoline and helicopters make it prohibitive to herd reindeer in much of central Chukotka, so that wild reindeer have room for expansion. Poaching is a major conservation problem. Poachers shoot wild reindeer from helicopters to obtain velvet antlers. Leaders of domestic reindeer cooperatives encourage poaching by telling people that wild reindeer are in fact just stray domestic reindeer and there is no enforcement of game laws.

  5. Evidence of combat in triceratops.

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    Andrew A Farke

    Full Text Available BACKGROUND: The horns and frill of Triceratops and other ceratopsids (horned dinosaurs are interpreted variously as display structures or as weapons against conspecifics and predators. Lesions (in the form of periosteal reactive bone, healing fractures, and alleged punctures on Triceratops skulls have been used as anecdotal support of intraspecific combat similar to that in modern horned and antlered animals. If ceratopsids with different cranial morphologies used their horns in such combat, this should be reflected in the rates of lesion occurrence across the skull. METHODOLOGY/PRINCIPAL FINDINGS: We used a G-test of independence to compare incidence rates of lesions in Triceratops (which possesses two large brow horns and a smaller nasal horn and the related ceratopsid Centrosaurus (with a large nasal horn and small brow horns, for the nasal, jugal, squamosal, and parietal bones of the skull. The two taxa differ significantly in the occurrence of lesions on the squamosal bone of the frill (P = 0.002, but not in other cranial bones (P > 0.20. CONCLUSIONS/SIGNIFICANCE: This pattern is consistent with Triceratops using its horns in combat and the frill being adapted as a protective structure for this taxon. Lower pathology rates in Centrosaurus may indicate visual rather than physical use of cranial ornamentation in this genus, or a form of combat focused on the body rather than the head.

  6. Concepts of the body and personhood in the Mesolithic-Neolithic Danube Gorges: interpreting animal remains from human burials

    Directory of Open Access Journals (Sweden)

    Ivana Živaljević

    2016-02-01

    Full Text Available In recent years, humanities have brought forward the idea of non-human agency; either in the form of meanings bestowed upon objects, animals and natural phenomena, or through deconstruction of ontological differences between ‘people’ and ‘things’. In case of the former, it has been argued that non-human agents have the power to act as ‘participants’ in social action (e.g. the agentive power of material properties of things, or of animal behaviour. In this paper, I discuss the practice of placing animal body parts alongside human bodies in the Mesolithic-Neolithic Danube Gorges, by using the concept of perspectivism as a theoretical framework. The choice of species and their body parts varied, but was by no means accidental. Rather, it reflected certain culturally specific taxonomies, which were based on animal properties: how they look, move, feel or what they do. Common examples include red deer antlers, which have the power to ‘regenerate’ each year, or dog mandibles (physical remains of ‘mouths’ which have the power to ‘communicate’ (i.e. bark. The aim of the paper is to explore how various aspects of animal corporeality, associated with certain ways of seeing and experiencing the world, could be ‘borrowed’ by humans utilizing animal body parts.

  7. Hunter perceptions and acceptance of alternative deer management regulations

    Science.gov (United States)

    Cornicelli, L.; Fulton, D.C.; Grund, M.D.; Fieberg, J.

    2011-01-01

    Wildlife managers are often confronted with a policy paradox where a majority of the public supports an outcome, but there is no agreement on specific management strategies to achieve this outcome. Previous research has also reported a link between regulatory acceptance, hunter satisfaction, and hunter participation rates. Thus, human dimensions research aimed at understanding hunter motivations and behavior is needed for effective management. In 2005, we surveyed Minnesota (USA) deer hunters (n = 6,000; 59% response) to evaluate attitudes regarding alternative deer (Odocoileus virginianus) harvest regulations. We also conducted a series of forced choice experiments in which respondents were asked to select an option from a list of representative regulations that might be adopted to achieve a particular deer management goal. Specifically, we modeled 5 deer population scenarios ranging from low populations with high buck-harvest rates to populations 50% over goal density. Our results indicate that hunters preferred different regulations depending on the population scenario, but generally preferred antler-point restrictions and disliked limiting buck licenses through a lottery. We also found consistency among scenarios, in that a small percentage of respondents indicated they would not hunt if regulations were changed. The results from this study should help wildlife managers design deer harvest regulations that are both acceptable to hunters and achieve management objectives. ?? 2011 The Wildlife Society.

  8. Unprecedented tools of animal origin in the prehistoric copper mines of La Profunda (León and their C14 dating (AMS

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    Miguel Angel de BLAS CORTINA

    2010-08-01

    Full Text Available La Profunda mine, in Leon, has been acknowledged for decades as a prehistoric mining, site generally attributed to the Bronze Age on account of the appearance of several copper axes (never duly described and which have now disappeared, a strange piece of the same metal and the characteristic, though chrono-culturally ambiguous pebble hammers. The recent finding of tools made from animal material, namely deer antler and tibia (Cervus elaphus as well as goat horn (Capra hircus, provide knowledge not only of a type of instrument well-documented in the significant copper mines of nearby Asturias (prehistoric workings at El Aramo and El Milagro, but also radiocarbon dating that situates the mining works of La Profunda in the second half of the third millennium B.C. This rich copper vein was to provide ore and metal to societies of a much greater scope than that corresponding to the mountainous area where the mines were opened, reaching Copper Age village communities (the so-called “Horizonte las Pozas” in the sedimentary lands of the northern plateau of Castile and also, probably, the authors of the Chalcolithic walled settlements that existed at the time in the south-eastern sector of the extensive Duero River Basin.

  9. Diversity and distribution of adeonid bryozoans (Cheilostomata: Adeonidae in Japanese waters

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    Masato Hirose

    2016-06-01

    Full Text Available Adeonid bryozoans construct antler-like erect colonies and are common in bryozoan assemblages along the Japanese Pacific coast. The taxonomy of Japanese adeonid species, however, has not been studied since their original descriptions more than 100 years ago. In the present study, adeonid specimens from historical collections and material recently collected along the Japanese coast are examined. Eight adeonid species in two genera were detected, of which Adeonella jahanai sp. nov., Adeonellopsis parvirostrum sp. nov., and Adeonellopsis toyoshioae sp. nov. are described as new species based on the branch width, size and morphology of frontal or suboral avicularia, shape and size of areolar pores, and size of the spiramen. Adeonellopsis arculifera (Canu & Bassler, 1929 is a new record for Japan. Lectotypes for Adeonellopsis japonica (Ortmann, 1890 and Adeonella sparassis (Ortmann, 1890 were selected among Ortmann’s syntypes. Most species of Adeonellopsis around Japan have a southern distribution from Sagami Bay to Okinawa, while A. japonica shows a more northern distribution from Kouchi to Otsuchi. In contrast, Adeonellopsis arculifera was collected only from southwestern Japan. A key to Japanese adeonid species is provided.

  10. Systematic review of the management of canine osteoarthritis.

    Science.gov (United States)

    Sanderson, R O; Beata, C; Flipo, R-M; Genevois, J-P; Macias, C; Tacke, S; Vezzoni, A; Innes, J F

    2009-04-01

    This review assesses the evidence for the efficacy of therapies used in the management of osteoarthritis in dogs on the basis of papers published in peer-reviewed journals in English between 1985 and July 2007. Sixty-eight papers were identified and evaluated. They considered four alternative therapies, one use of functional food, two intra-articular agents, six nutraceutical agents, 21 pharmacological agents, two physical therapies, three surgical techniques and two combinations of weight control. There was a high level of comfort (strong evidence) for the efficacy of carprofen, firocoxib and meloxicam, and a moderate level of comfort for the efficacy of etodolac in modifying the signs of osteoarthritis. There was a moderate level of comfort for the efficacy of glycosaminoglycan polysulphate, licofelone, elk velvet antler and a functional food containing green-lipped mussel for the modification of the structures involved in the disease. There was weak or no evidence in support of the use of doxycycline, electrostimulated acupuncture, extracorporeal shockwave therapy, gold wire acupuncture, hyaluronan, pentosan polysulphate, P54FP (extract of turmeric), tiaprofenic acid or tibial plateau levelling osteotomy. PMID:19346540

  11. Aggression and coexistence in female caribou

    Science.gov (United States)

    Weckerly, Floyd W.; Ricca, Mark A.

    2014-01-01

    Female caribou (Rangifer tarandus) are highly gregarious, yet there has been little study of the behavioral mechanisms that foster coexistence. Quantifying patterns of aggression between male and female, particularly in the only cervid taxa where both sexes grow antlers, should provide insight into these mechanisms. We asked if patterns of aggression by male and female caribou followed the pattern typically noted in other polygynous cervids, in which males display higher frequencies and intensity of aggression. From June to August in 2011 and 2012, we measured the frequency and intensity of aggression across a range of group sizes through focal animal sampling of 170 caribou (64 males and 106 females) on Adak Island in the Aleutian Archipelago, Alaska. Males in same-sex and mixed-sex groups and females in mixed-sex groups had higher frequencies of aggression than females in same-sex groups. Group size did not influence frequency of aggression. Males displayed more intense aggression than females. Frequent aggression in mixed-sex groups probably reflects lower tolerance of males for animals in close proximity. Female caribou were less aggressive and more gregarious than males, as in other polygynous cervid species.

  12. What enables size-selective trophy hunting of wildlife?

    Directory of Open Access Journals (Sweden)

    Chris T Darimont

    Full Text Available Although rarely considered predators, wildlife hunters can function as important ecological and evolutionary agents. In part, their influence relates to targeting of large reproductive adults within prey populations. Despite known impacts of size-selective harvests, however, we know little about what enables hunters to kill these older, rarer, and presumably more wary individuals. In other mammalian predators, predatory performance varies with knowledge and physical condition, which accumulates and declines, respectively, with age. Moreover, some species evolved camouflage as a physical trait to aid in predatory performance. In this work, we tested whether knowledge-based faculty (use of a hunting guide with accumulated experience in specific areas, physical traits (relative body mass [RBM] and camouflage clothing, and age can predict predatory performance. We measured performance as do many hunters: size of killed cervid prey, using the number of antler tines as a proxy. Examining ∼ 4300 online photographs of hunters posing with carcasses, we found that only the presence of guides increased the odds of killing larger prey. Accounting for this effect, modest evidence suggested that unguided hunters presumably handicapped with the highest RBM actually had greater odds of killing large prey. There was no association with hunter age, perhaps because of our coarse measure (presence of grey hair and the performance trade-offs between knowledge accumulation and physical deterioration with age. Despite its prevalence among sampled hunters (80%, camouflage had no influence on size of killed prey. Should these patterns be representative of other areas and prey, and our interpretations correct, evolutionarily-enlightened harvest management might benefit from regulatory scrutiny on guided hunting. More broadly, we suggest that by being nutritionally and demographically de-coupled from prey and aided by efficient killing technology and road access

  13. Le comportement symbolique dans la Préhistoire de la Roumanie: art mobilier au Paléolithique supérieur en Moldavie

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    Corneliu BELDIMAN

    2009-12-01

    in a cave site. The types identified are: bâtons percés worked in horse long bone and in roe-deer antler; lithic and bone pendants; disk in roe-deer antler; perforated teeth; lithic objects in quartzite and graphite as well as bone and antler pieces having linear engraved decoration or notches; decorated roe-deer antler harpoon: ivory mammoth tusk fragment; fossil mollusks of Congeria? species; perforated snails of Succinea oblonga? ou Lithoglyphus naticoides? species. Most of the artifacts are of significant importance for the phenomenon of art and of prehistoric technology in these regions; in this point of view we have to mention the pendants discovered at Mitoc, Botosani County and the fragment of bone discovered in 1998 with the engraved image of an animal’s foot from Piatra Neamt, Neamt County. Another exceptional artifact is the fragment of mammoth tusk from Lespezi, Bacau County dated at around 18 kya showing the débitage traces on the proximal part that prove the using of notching and grooving technique and probably of transverse sawing with fiber; this should be the oldest situation of use of such a technique solution in this part of Europe. Taking into account the extreme rarity of ivory artifacts in the Upper Paleolithic of Romania it is probably that the provenance of the objects can be found in the near territories of Central and Eastern Europe (Czech Republic, Ukraine, Republic of Moldavia, Russia where the manufacture and use of such artifacts was common in that epoch. The study contributes essentially to the definition in actual terms of typology and technology of oldest portable art objects from a particular region of Romania as material expression of first spiritual manifestations of huntergatherer communities and allowed to integrate the data of the phenomenon in the South-East and Central European context.

  14. Health status and relative exposure of mule deer and white-tailed deer to soil contaminants at the rocky mountain arsenal

    Science.gov (United States)

    Creekmore, T.E.; Whittaker, D.G.; Roy, R.R.; Franson, J.C.; Baker, D.L.

    1999-01-01

    We evaluated the health of 18 radio-collared deer [13 mule deer (Odocoileus hemionus) and 5 white-tailed deer (O. virginianus)] from the Rocky Mountain Arsenal, near Denver, Colorado, USA, a Superfund site contaminated with a variety of materials, including organochlorine pesticides, metals, and nerve gas production by-products. Radio-collared deer were tracked for 1 to 3 years (1989-1992) to identify relative exposure to contaminants based on telemetry locations plotted on grid maps depicting known soil contaminant concentrations. At the end of the study, all animals were in fair or good body condition at the time of necropsy. Mean ages of mule deer and white-tailed deer were 7.4 (range 4-12) and 10.6 years (range 5-17), respectively. At necropsy, tissues were collected from the deer for serology, histopathology, and analysis for eight chlorinated hydrocarbons and two metals. Detectable residues of mercury were found in the kidneys of 10 deer (range 0.055-0.096 ??g/g), dieldrin was found in fat (n = 9) (range 0.02-0.72 ??g/g), liver (n = 4) (range 0.017-0.12 ??g/g), and brain (n = 1, 0.018 ??g/g), and DDE was found in the muscle of one animal (0.02 ??g/g). Relative exposure estimates derived from telemetry and soil contamination data were correlated with tissue levels of dieldrin (p < 0.001) and mercury (p = 0.05). Two mule deer had severe testicular atrophy, and one of these animals also had antler deformities. The prevalence of antibodies against epizootic hemorrhagic disease serotype 2 was 85%.

  15. Trading of crude drugs between China and Russia as recorded in 3 Chinese versions of Russian works%3部汉译俄文文献记载的中俄药材贸易

    Institute of Scientific and Technical Information of China (English)

    丁兆平; 王振国

    2014-01-01

    19世纪前,中俄两国贸易中涉及到中药材的进出口往来.由中国输入俄国的中药材以植物性来源为主,其中以大黄为最重要的贸易品种,其次为药食两用的辛香料如八角茴香、桂皮、胡椒、姜,还有特殊的药用品种如当归、奎宁.而由俄国输入中国的药材则以动物性来源为主,其中尤以贵重的动物性中药麝香在1854年的大量输入特别引入注目,曾一次性输入7万个麝香囊,其他品种如鹿茸、羚羊角的贸易也典型体现了俄方的地域与物产特点.%Trading of crude drugs between China and Russia appeared before the 19th century.By then,exports of Chinese crude drugs to Russia were mainly plants,with Radix et Rhizoma Rhei as the most exported one,followed by both dietary and medicinal aromatic plants,such as fennel,cinnamon bark,pepper,ginger,and Radix Angelica sinensis,and quinine.Imports of Russian drugs were mainly of animal ones,especially those valuable animals like musk sac.In 1854,the importation of a large amount of such sac,numbering as many as 70 000,was very remarkable.Other drugs includcd stag antler,tender horn of deer,demonstrating typically the regional and productive characteristics of Russia.

  16. Upper Palaeolithic ritualistic cannibalism at Gough's Cave (Somerset, UK): The human remains from head to toe.

    Science.gov (United States)

    Bello, Silvia M; Saladié, Palmira; Cáceres, Isabel; Rodríguez-Hidalgo, Antonio; Parfitt, Simon A

    2015-05-01

    A recurring theme of late Upper Palaeolithic Magdalenian human bone assemblages is the remarkable rarity of primary burials and the common occurrence of highly-fragmentary human remains mixed with occupation waste at many sites. One of the most extensive Magdalenian human bone assemblages comes from Gough's Cave, a sizeable limestone cave set in Cheddar Gorge (Somerset), UK. After its discovery in the 1880s, the site was developed as a show cave and largely emptied of sediment, at times with minimal archaeological supervision. Some of the last surviving remnants of sediment within the cave were excavated between 1986 and 1992. The excavations uncovered intensively-processed human bones intermingled with abundant butchered large mammal remains and a diverse range of flint, bone, antler, and ivory artefacts. New ultrafiltrated radiocarbon determinations demonstrate that the Upper Palaeolithic human remains were deposited over a very short period of time, possibly during a series of seasonal occupations, about 14,700 years BP (before present). The human remains have been the subject of several taphonomic studies, culminating in a detailed reanalysis of the cranial remains that showed they had been carefully modified to make skull-cups. Our present analysis of the postcrania has identified a far greater degree of human modification than recorded in earlier studies. We identify extensive evidence for defleshing, disarticulation, chewing, crushing of spongy bone, and the cracking of bones to extract marrow. The presence of human tooth marks on many of the postcranial bones provides incontrovertible evidence for cannibalism. In a wider context, the treatment of the human corpses and the manufacture and use of skull-cups at Gough Cave have parallels with other Magdalenian sites in central and western Europe. This suggests that cannibalism during the Magdalenian was part of a customary mortuary practice that combined intensive processing and consumption of the bodies with

  17. Tectonostratigraphic terranes of the frontier circum-Pacific region

    Energy Technology Data Exchange (ETDEWEB)

    Howell, D.G.; Jones, D.L.; Schermer, E.R.

    1983-03-01

    Many major exploration frontiers around the Pacific are in regions where complex geologic relations reflect plate-tectonic processes, crustal mobility, and accretion of exotic terranes. The destruction of the proto-Pacific ocean (Panthalassa) involved accretion of terranes to cratonal regions such as Gondwana and Laurasia. Terranes in southwestern New Zealand and eastern Antarctica were also probably accreted during the Paleozoic. The southern margin of Siberia, extending into China, underwent a protracted period of accretion from the late Precambrian through the early Mesozoic. Mid-Paleozoic accretion is reflected in the Innuitian foldbelt of the Arctic Ocean, the Black Clastic unit of the northern Rocky Mountains, and the Antler orogeny of the western US cordillera. The Mesozoic breakup of Pangaea and the acceleration of subduction aided in the rifting and dispersal of terranes from equatorial paleolatitudes. Fragments of these terranes now compose much of the continental margins of the Pacific basin, including New Zealand, Indochina, southern China, southeast Siberia, the North American cordillera, and South America. Some terranes are presently being further fragmented by post-accretionary dispersion processes such as strike-slip faulting in western North America and Japan. Although the character and distribution of terranes in the western US are fairly well documented, details are needed for other terranes around the Pacific basin. Interpretation of structure and stratigraphy at depth will be aided by more data on the timing of accretion and the nature of deformation associated with accretion and dispersion. Such data are needed for further define specific exploration targets in the circum-Pacific region.

  18. Transcriptome analysis of sika deer in China.

    Science.gov (United States)

    Jia, Bo-Yin; Ba, Heng-Xing; Wang, Gui-Wu; Yang, Ying; Cui, Xue-Zhe; Peng, Ying-Hua; Zheng, Jun-Jun; Xing, Xiu-Mei; Yang, Fu-He

    2016-10-01

    Sika deer is of great commercial value because their antlers are used in tonics and alternative medicine and their meat is healthy and delicious. The goal of this study was to generate transcript sequences from sika deer for functional genomic analyses and to identify the transcripts that demonstrate tissue-specific, age-dependent differential expression patterns. These sequences could enhance our understanding of the molecular mechanisms underlying sika deer growth and development. In the present study, we performed de novo transcriptome assembly and profiling analysis across ten tissue types and four developmental stages (juvenile, adolescent, adult, and aged) of sika deer, using Illumina paired-end tag (PET) sequencing technology. A total of 1,752,253 contigs with an average length of 799 bp were generated, from which 1,348,618 unigenes with an average length of 590 bp were defined. Approximately 33.2 % of these (447,931 unigenes) were then annotated in public protein databases. Many sika deer tissue-specific, age-dependent unigenes were identified. The testes have the largest number of tissue-enriched unigenes, and some of them were prone to develop new functions for other tissues. Additionally, our transcriptome revealed that the juvenile-adolescent transition was the most complex and important stage of the sika deer life cycle. The present work represents the first multiple tissue transcriptome analysis of sika deer across four developmental stages. The generated data not only provide a functional genomics resource for future biological research on sika deer but also guide the selection and manipulation of genes controlling growth and development. PMID:27423230

  19. CONSERVATION DEVELOPMENT OF TIMOR DEER (Cervus timorensis AS COMMERCIAL PURPOSE(WITH OPTIMISTIC RATE ESTIMATION

    Directory of Open Access Journals (Sweden)

    N. Hanani

    2012-09-01

    Full Text Available The aim of this research was to determine the profit obtained from breeding of Timor deer commercially. This research was done in East Java. Survey method was used to answer the objective. The study location were selected by purposive sampling. Usually deer was develop in conservation area, but because the area was decrease so the number of deer also decrease. Model of deer raising development should be improved not only for conservation but also for commercial purpose. The optimum deer raising were considered and monitored with a purpose to maximize commercial Timor deer by using Multiple Objective Goal Programming (MOGP to find the Optimistic Rate Estimation. The result of this study showed to get the optimum benefit, it had to be applied together with conservation and commercial effort at the same time. Results of study showed that profit was taken from selling velvet was 164.46%. Profits taken from selling antler was 350.56%, from selling alive deer was 394.28%, from selling recreation tickets was 259.08%, from selling venison1 was 135.98%, and from selling deer leather was 141.24%. Operational cost spent were 168.46% for feeding cost, 213.23% for maintenance cost, and 232.04% for labors’ salaries. The amount of operational cost required in MOGP model, with lower expenses and commercial priority were 185.54% for feeding cost, 253.13% for maintenance cost, and 246.95% for paying labors’ salaries. The MOGP model result with commercial priority reached 335.21%, while in MOGP model with lower costs and commercial priority gave profit for breeders up to 381.26%.

  20. Detrital zircon geochronology of Siluro-Devonian sandstones, Rocky Mountains, northeastern British Columbia

    Energy Technology Data Exchange (ETDEWEB)

    Ross, G.M.; Geldsetzer, H.H.J. (Inst. of Sedimentary and Petroleum Geology, Calgary, AB (Canada)); McNicoll, V.J.; Parrish, R.R. (Geological Survey of Canada, Ottawa, ON (Canada)); Carr, S.D; Kinsman, A. (Carleton Univ., Ottawa, ON (Canada))

    1993-09-01

    Quartzose sandstones of latest Silurian to middle Devonian form the basal part of four unconformity-bounded, carbonate dominated shelf successions of the Alberta Basin in the Rocky Mountains of northeastern British Columbia; the Muncho-McConnell, Stone, Dunedin and Slave Point formations. The off-shelf equivalent to the Dunedin Formation is a massive channel deposit of quartzose sandstone that overlies basinal strata along a 30 km wide re-entrant in the northern part of the Ospika Embayment. Paleocurrent measurements from basal sandstones of the Dunedin Formation, including the channel deposit, contain a northeast-directed component over a large area and suggest possible sediment derivation from a western source area, perhaps reflecting uplift during the early phases of the Antler orogeny. To test this hypothesis, single detrital zircon grains were dated using the U-Pb technique, from sandstones of the shelf (Muncho-McConnell and Slave Point formations) and basinal settings (massive channel deposit) to examine their provenance. Twenty-nine single grain analyses from four different samples yielded a distinctive suite of U-Pb ages that are undistinguishable regardless of paleogeographic setting and closely match the ages of basement terranes that underlie the Peace River Arch in the autocthon to the east. These data support derivation of sediments of both basinal and shelf associations from the same source: the emergent Peace River Arch area to the east. Alternatively, to account for the northeasterly directed paleocurrents, Dunedin-equivalent quartzose sandstones of the basinal association could have been derived from the west and acquired their detrital zircon provenance signature by recycling pre-Devonian sandstones. 29 refs., 7 figs., 1 tab.

  1. Evaluating the transitional mosaic: frameworks of change from Neanderthals to Homo sapiens in eastern Europe

    Science.gov (United States)

    Davies, William; White, Dustin; Lewis, Mark; Stringer, Chris

    2015-06-01

    Defining varying spatial and temporal analytical scales is essential before evaluating the responses of late Neanderthals and early Homo sapiens to Abrupt Environmental Transitions (AETs) and environmental disasters for the period 130-25 ka. Recent advances in addressing the population histories and interactions (using both genetic and archaeological evidence) of Neanderthals and H. sapiens have encouraged consideration of more subtle dynamics of archaeological change. Descriptions of change based on methodologies pioneered some 160 years ago are no longer adequate to explain the patterning we now see in the record. New chronological results, using multiple dating methods, allow us to begin to unpick the spatial and temporal scales of change. Isochronic markers (such as specific volcanic eruptions) can be used to create temporal frameworks (lattices), and results from other dating techniques compared against them. A combination of chronological lattices and direct dating of diagnostic artefacts and human fossils permits us, for the first time, to have greater confidence in connecting human (recent hominin) species and their behavioural responses to environmental conditions, and in quantifying scales of change over time and space (time-transgression). The timing of innovations, particularly those in bone, antler and ivory, can be directly quantified and tested, and used to re-evaluate longstanding models of cultural change. This paper also uses these new chronologies to explore the ecologies of late Neanderthals and early H. sapiens: their population densities, mobilities, resources exploited and possible interactions. Environmental productivity estimates are used to generate new questions of potential population densities and mobilities, and thus the sensitivity of these groups to environmental perturbations. Scales and intensities of effect on environments from natural disasters and AETs (notably Heinrich Events and the Campanian Ignimbrite eruption) are defined

  2. Evaluation of water resources in part of central Texas

    International Nuclear Information System (INIS)

    Water resources in the Brazos, Red, Colorado, and Trinity River basins, in central Texas, were evaluated. In 1985 there was a little less than 81,000 acre-ft of groundwater pumped from all aquifers in the study area, with a little less than 77,000 acre-ft of groundwater pumped from the Trinity Group aquifer. Irrigation accounted for about 56% of all groundwater pumped. A serious problem associated with the development of groundwater from the Trinity Group aquifer is the decline of artesian pressure in areas of large groundwater withdrawals. Degradation of groundwater within the Antlers and Travis Peak Formations from oil-field brines and organic material are problems in several counties. The deterioration of water qualify for the City of Blum has occurred over a 26-year period and is associated with water level declines in the Hensell Member of the Travis Peak Formation. The Woodbine Group yields good quality water at or near the outcrop; however, the residual sodium carbonate and percent sodium limits its use for irrigation, while high iron and fluoride content restricts its use for public supply. Existing surface reservoirs in the study area alone can supply 296,400 acre-ft of water under 2010 conditions. Nearly all of this water is either currently owned or under contract to supply current and future needs. An additional 176,000 acre-ft of surface water could become available with the development of the proposed Lake Bosque and Paluxy Reservoir projects and with reallocation of storage in existing Lakes Waco and Whitney. The amount of groundwater currently pumped exceeds the estimated annual effective recharge to the Trinity Group aquifer; the groundwater supply for the area will continue to be drawn from storage within the aquifer. 84 refs., 21 figs., 3 tabs

  3. Evaluation of water resources in part of north-central Texas

    International Nuclear Information System (INIS)

    Water resources within the North-Central Texas Region which lies in the Brazos, Red, Sulphur, Sabine, and Trinity River basins, were evaluated. The geologic formations underlying the study area range in age from Paleozoic to Recent. The principal water-bearing formations are the Antlers, Twin Mountains, and Paluxy Formations of the Trinity Group, and the Woodbine Group, all of Cretaceous age. Extensive cones of depression have developed in the piezometric surface of each of the region's principal aquifers, coinciding with areas of large groundwater withdrawals. During the 13-year period 1976 to 1989, water level declines of 25 ft were common in the aquifers studied. The study identified a number of existing and potential water quality problems. Some of these are naturally occurring and are related to geologic structure. Elevated levels of some chemical constituents suggest contamination related to oil-field activities and other industrial sources. The projected water demands for the study area by the year 2010 total over 1.4 million acre-ft. Existing surface-water reservoirs can supply 1.6 million acre ft of water under 2010 conditions. The amount of groundwater currently pumped exceeds the estimated annual effective recharge to the area's aquifers; a portion of the water pumped is drawn from aquifer storage resulting in water level declines. Even though the study area has sufficient surface water to meet projected needs through 2010, rural areas that depend on a groundwater supply could face shortages before 2010 due to the limited availability of groundwater and the high cost of converting to a surface water supply. 93 refs., 19 figs., 3 tabs

  4. Effect of Shengdeye on anti-fatigue and the memory in mice

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    AIM: Shengdeye is a kind of medicinal wine. It is fermented with 30 kinds of medicinal herbs such as glossy ganoderma, pilose antler, panax, medlar and sorghum. This study aimed to observe the effects of Shengdeye on anti-fatigue and memory in mice.METHODS: The mice were fed with Shengdeye 5 ml*kg-1 and 2.5 ml*kg-1 2 times per day. After 7 days, the mice were examined on anoxia-resistant and swimming test for anti-fatigue and step down test for memory.RESULTS: The anoxia-resistant of shengdege group was apparently longer than that of the control group [(29.60±1.36) min vs (24.40±3.13) min, P<0.01], and the swimming time was sharply increased [(142.6±53.8) min for 2.5 ml*kg-1 and (162.9±43.5) min for 5 ml*kg-1 vs (94.9±39.1) min, P<0.05]. The error times of shengdege group was lower than that of the control [(1.5±1.4) times and (2.3±1.3) times vs (3.7±1.1) times, P<0.01]. The delitescence of two group were sharply prolonged [(136.8±50.1) s, and (128.0±41.5) s vs (50.2±42.9) s, P<0.01].CONCLUSION: The results indicated that Shengdeye had an anti-fatigne effect and increased the faculty of memory. The experiment of acute toxicity showed that the medicinal herbs in Shyengdeye were safe.

  5. The Population Status and Potential Resources' Utilization of Elaphurus davidianus%中国麋鹿种群现状与潜在资源利用

    Institute of Scientific and Technical Information of China (English)

    张树苗; 梁兵宽; 张林源; 李坤

    2009-01-01

    Elaphurus davidianus is one special species in Chinese deer family, belonging to 1st class of protected animals. Its antler, fat, flesh and blood etc possess medical values. Since human being's irregular hunting and environmental changes, Elaphurus davidianus had been become extinct since 1900. In the middle of 1980s, our country initiated Milu's reintroduction program, which made the quantity of Milu over 2000 individuals. The paper analyzes Elaphurus davidianus' values in food, medicine, leather, medical care, beauty, ecological and cultural aspects,and issues in its population development such as kin mating, few distribution spot, density restrict, as well as measures for its protection and sustainable utilization.%麋鹿是中国特有的鹿科动物,是国家Ⅰ级保护动物,其茸、角、脂、血、肉等均具有药用价值.由于人类的猎捕和环境变迁,麋鹿于1900年灭绝.20世纪80年代中期,我国启动了麋鹿重引进项目,现在我国麋鹿数量超过了2 000头.分析麋鹿在食用、药用、制革、保健与美容、观赏、生态及文化方面的价值及目前种群发展存在着的近亲交配、分布点少、密度制约等问题,提出了保护与可持续利用对策.

  6. Silkworm Bioreactor Industrialization System and Product Development%家蚕生物反应器产业化体系与产品开发

    Institute of Scientific and Technical Information of China (English)

    于威; 李春义; 张志芳; 禇文辉

    2016-01-01

    At present there are several successful bioreactors which can realize industrial mass production. As one of the bioreactor system, insect baculovirus expression system can express many biologicaly active mammalian genes. It has obvious advantages due to the relatively low cost in production when compared to other expression systems. Silkworm is the only economic insect which can carry out this work and realize mass rearing. The recombinant baculovirus can be constructed by gene recombination in vitro and then the foreign gene was expressed in insect cels or larvae. This method has the advantages of simple operation, high expression quantity and good stability. Moreover, silkworm bioreactor, with Chinese characteristics, is currently the most promising insect baculovirus expression system to efficient produce biological products. We first put forward the idea to produce oral drug using silkworm pupa to express hGM-CSF gene. The animal oral experiments showed that the the white cel level of mice increased significantly after chemotherapy. This drug was approved for clinical trials by the SFDA in 2004. It has great theoretical significance and application prospect about this change of delivery form. The drug scale preparation technology of silkworm bioreactor is one of the key technologies to realize the industrial mass production of target protein drugs. Its technical level is also the key point of drugs in market price competition. Velvet antler is the head derivatives of Cervidae animal, the growth rate is amazing. It can be used as organ bioreactor to produce the expensive biological factors. The production of velvet antler will contain the active ingredient of its own and other expensive medicinal substances. Using this bioreactor to produce recombinant protein has great potential market. At present based on the silkworm bioreactor and transgenic mammalian organs bioreactor (antler), we use silkworm bioreactor to produce heterologous protein and virus vaccine in

  7. Modelling the Loktak Lake Basin to Assess Human Impact on Water Resources

    Science.gov (United States)

    Eliza, K.

    2015-12-01

    Loktak Lake is an internationally important, Ramsar designated, fresh water wetland system in the state of Manipur, India. The lake was also listed under Montreux Record on account of the ecological modifications that the lake system has witnessed over time. A characteristic feature of this lake is the extensive occurrence of coalesced, naturally or otherwise, vegetative masses floating over it. A contiguous 40 km2 area of Phumdis, as these vegetative masses are locally referred to, also constitutes the only natural home of the endemic and endangered species of Manipur's brow-antlered deer popularly known as Sangai. Appropriately notified as Keibul Lamjao National Park by Government of India, this natural feature is known to be the world's largest floating park. Water quality and sediment deposition on account of soil erosion in its catchments are some of the emerging concerns along with a reported enhanced frequency and duration of flooding of the shore areas, reduced fish catch within a visibly deteriorated overall natural ecosystem. Disturbances of watershed processes, command area management practices, ineffective as indeed largely absent, waste management practices and management interventions linked to the Loktak Hydroelectric Project are often cited as the principal triggers that are seen to be responsible for the damage. An effective management protocol for the Lake requires a rigorous understanding of its hydrobiology and eco-hydrodynamics. The present study is carried out to establish such a characterization of the various rivers systems draining directly into the Lake using MIKE SHE, MIKE 11 HD and MIKE 11 ECO Lab modelling platforms. Water quality modelling was limited to dissolved oxygen (DO), biological oxygen demand (BOD) and water temperature. Model calibration was done using the available measured water quality data. The derived results were then investigated for causal correlation with anthropogenic influences to assess human impact on water

  8. Biostratigraphy and structure of paleozoic host rocks and their relationship to Carlin-type gold deposits in the Jerritt Canyon mining district, Nevada

    Science.gov (United States)

    Peters, S.G.; Armstrong, A.K.; Harris, A.G.; Oscarson, R.L.; Noble, P.J.

    2003-01-01

    The Jerritt Canyon mining district in the northern Independence Range, northern Nevada, contains multiple, nearly horizontal, thrust masses of platform carbonate rocks that are exposed in a series of north- to northeast-elongated, tectonic windows through rocks of the Roberts Mountains allochthon. The Roberts Mountains allochthon was emplaced during the Late Devonian to Early Mississippian Antler orogeny. These thrust masses contain structurally and stratigraphically controlled Carlin-type gold deposits. The gold deposits are hosted in tectonically truncated units of the Silurian to Devonian Hanson Creek and Roberts Mountains Formations that lie within structural slices of an Eastern assemblage of Cambrian to Devonian carbonate rocks. In addition, these multiply thrust-faulted and folded host rocks are structurally interleaved with Mississippian siliciclastic rocks and are overlain structurally by Cambrian to Devonian siliciclastic units of the Roberts Mountains allochthon. All sedimentary rocks were involved in thrusting, high-angle faulting, and folding, and some of these events indicate substantial late Paleozoic and/or Mesozoic regional shortening. Early Pennsylvanian and late Eocene dikes also intrude the sedimentary rocks. These rocks all were uplifted into a northeast-trending range by subsequent late Cenozoic Basin and Range faulting. Eocene sedimentary and volcanic rocks flank part of the range. Pathways of hydrothermal fluid flow and locations of Carlin-type gold orebodies in the Jerritt Canyon mining district were controlled by structural and host-rock geometries within specific lithologies of the stacked thrust masses of Eastern assemblage rocks. The gold deposits are most common proximal to intersections of northeast-striking faults, northwest-striking dikes, and thrust planes that lie adjacent to permeable stratigraphic horizons. The host stratigraphic units include carbonate sequences that contained primary intercrystalline permeability, which

  9. Drug reaction with eosinophilia and systemic symptoms syndrome probably induced by a lamotrigine-ginseng drug interaction.

    Science.gov (United States)

    Myers, Amy P; Watson, Troy A; Strock, Steven B

    2015-03-01

    The likelihood of a drug reaction with lamotrigine is increased by dose escalation that is too rapid or drug interactions that increase the concentration of lamotrigine. There is a well-documented interaction between valproic acid and lamotrigine in which lamotrigine levels are increased, subsequently increasing the risk of a drug reaction with eosinophilia and systemic symptoms (DRESS) syndrome. This syndrome is characterized by fever, lymphadenopathy, diffuse maculopapular rash, multivisceral involvement, eosinophilia, and atypical lymphocytes and has a mortality rate of 10-40%. We describe the first case, to our knowledge, of DRESS syndrome that was probably induced by a drug interaction between lamotrigine and ginseng. A 44-year-old white man presented to the emergency department after experiencing a possible seizure. His medical history included two other lifetime events concerning for seizures at ages 14 and 29 years old. After referral to the neurology clinic, he was diagnosed with generalized tonic-clonic seizure disorder, and lamotrigine was started with up-titration according to the drug's package insert to a goal dosage of 150 mg twice/day. The patient had also been taking deer antler velvet and ginseng that he continued during his lamotrigine therapy. On day 43 of therapy, the patient presented to the emergency department with a pruritic rash that had started on his extremities and spread to his torso. He was thought to have experienced a drug reaction to lamotrigine, and the drug was discontinued. Thirteen days later, the patient was admitted from the acute care clinic for inpatient observation due to laboratory abnormalities in the setting of continued rash, headache, and myalgias. His admission laboratory results on that day were remarkable for leukocytosis, with a white blood cell count up to 17.6 × 10(3) /mm(3) , with a prominent eosinophilia of 3.04 × 10(3) /mm(3) ; his liver enzyme levels were also elevated, with an aspartate

  10. 鹿食毛症的发病原因与综合防治措施%On the Pathogenesis and Control Measures of Deer Eat-feather-disease

    Institute of Scientific and Technical Information of China (English)

    宋百军; 毕继宏; 刘伍权

    2012-01-01

    The lack of minerals,trace elements,sulfur-containing amino acids and other nutrients or stocking density is too large caused by deer bite hair,food hair and secondary stomach flaccid,intestinal obstruction,a major cause.General incidence of March to April,the incidence of more than 90%,resulting in the decline of the male deer antler quality and yield,fawn stunting,severe and even formed in the stomach and small intestine of the true size,ranging from hairballs,blocking the digestive tract cause death,serious harm to deer industry,resulting in huge economic losses.The main control measures have reasonable clustering management,appropriate stocking density,supplementary minerals and sulfur amino acids,the balance of feed nutrition,sick deer can only be orally laxatives to exclude the hair bulb,a serious need surgery.%矿物质、微量元素、含硫氨基酸等营养物质缺乏或饲养密度过大是引起鹿只咬毛、食毛并继发前胃弛缓、瓣胃阻塞的主要病因。鹿食毛症一般多在3~4月份发病,发病率高达90%以上,导致公鹿鹿茸品质和产量下降,仔鹿生长发育不良,严重的甚至在真胃和小肠内形成大小不等的毛团,阻塞消化道造成死亡,给养鹿业带来严重危害,造成经济上的巨大损失。主要防治措施有合理分群管理,饲养密度适当;补充矿物元素和含硫氨基酸,平衡饲料营养;患病鹿只可以灌胃泻药排除毛球,严重的需要手术治疗。

  11. Viability of Timor deer stag (Cervus timorensis spermatozoa extended in tris egg yolk diluent with different sources of carbohydrate and storage at room temperature

    Directory of Open Access Journals (Sweden)

    W. Marlene Mesang-Nalley

    2007-12-01

    Full Text Available The successful sperm preservation, influenced by the capability of its extender on the maintenance the sperm quality during storage. The carbohydrate such as glucose and fructose were the common sugar added on the mammalian sperm extender to support their live and motility. The sucrose was the main carbohydrate in Timor deer stag seminal plasma. The experiment was conducted to evaluate the effect of carbohydrates in Tris egg yolk (TEY extender on the motility and viability of stag sperm, stored in room temperature (27-28 oC. The semen was collected using electro ejaculator from five Timor deer stags at hard antler stage, 3-5 years old, body weight of 64-102 kg with normal testes. The semen was than evaluated macro-and microscopically and divided into 3 aliquots. Each of them was diluted with TEY-glucose (TEYG, TEY-fructose (TEYF and TEY-Sucrose (TEYS with the concentration of spermatozoa 100 x 106 ml-1. The extended semen was than stored at room temperature. The sperm motility and viability were evaluated every 3 hours. Result of the experiment showed that the semen volume was 2.06 ± 0.63 ml, pH 7.03±0.13, yellow white until creamy in color and the consistency ranged from normal to thick. The mass movement between ++ to +++ and the sperm motility was 68.67 ± 7.4%. The average of sperm concentration was 842.35 ± 258.14x106 ml-1, the viable sperm was 78.11 ± 3.61%, the sperm abnormality was 7.31 ± 2.98%. The percentages of sperm motility on TEYG (18.00 ± 17.63% and TEYS (21.83 ± 15.92% were higher compare to TEYF (4,00 ± 0,00% extender in 24 hours observation. The percentage of sperm viability showed the same pattern. The sperm viability in TEYG (28.17 ± 20.06 and TEYS (24.00 ± 22.59% (P<0.05 were significantly higher compare to TEYF (4.00 ± 0.00%. It is concluded that the deer stag sperm can use the three sugars for their nutrition source. The diluted sperm still can be used for artificial insemination after 12 hour storage.

  12. Uncertainty in age-specific harvest estimates and consequences for white-tailed deer management

    Science.gov (United States)

    Collier, B.A.; Krementz, D.G.

    2007-01-01

    Age structure proportions (proportion of harvested individuals within each age class) are commonly used as support for regulatory restrictions and input for deer population models. Such use requires critical evaluation when harvest regulations force hunters to selectively harvest specific age classes, due to impact on the underlying population age structure. We used a stochastic population simulation model to evaluate the impact of using harvest proportions to evaluate changes in population age structure under a selective harvest management program at two scales. Using harvest proportions to parameterize the age-specific harvest segment of the model for the local scale showed that predictions of post-harvest age structure did not vary dependent upon whether selective harvest criteria were in use or not. At the county scale, yearling frequency in the post-harvest population increased, but model predictions indicated that post-harvest population size of 2.5 years old males would decline below levels found before implementation of the antler restriction, reducing the number of individuals recruited into older age classes. Across the range of age-specific harvest rates modeled, our simulation predicted that underestimation of age-specific harvest rates has considerable influence on predictions of post-harvest population age structure. We found that the consequence of uncertainty in harvest rates corresponds to uncertainty in predictions of residual population structure, and this correspondence is proportional to scale. Our simulations also indicate that regardless of use of harvest proportions or harvest rates, at either the local or county scale the modeled SHC had a high probability (>0.60 and >0.75, respectively) of eliminating recruitment into >2.5 years old age classes. Although frequently used to increase population age structure, our modeling indicated that selective harvest criteria can decrease or eliminate the number of white-tailed deer recruited into older

  13. Dating of Las Mesas Copper Age walled enclosure (La Fuente, Spain

    Directory of Open Access Journals (Sweden)

    Odriozola, Carlos P.

    2014-12-01

    Full Text Available The site of Las Mesas is located in the west bank of the Guadajira river (La Fuente, Badajoz, Spain. It covers a half hectare and is enclosed by a stone wall. Previous assessments based on site surveys suggested a general chronological span from Late Neolithic period (ca. 3600-2900 BC through the Copper Age (3rd millennium BC. Excavations revealed a well-constructed wall with bastions and a domestic space at the centre of the site with three occupational phases. Several functional areas and a votive pit are ascribed to the second phase. A deer antler located in a re-cut ritual pit associated to the second phase was dated by AMS-14C. Samples from a living floor assigned to the second phase (SU-9/10 and the basement of a kiln associated with the first phase (SU-18 was dated by optically stimulated luminescence (OSL using the single aliquot regenerative dose (SAR protocol on 90-160 m quartz grains. Luminescence profiling analysis of 7 samples taken throughout the archaeological stratigraphy was used to assess variations in luminescence behaviour and relative age through the sequence, using infrared-, optically –and thermally– stimulated luminescence (IRSL, OSL, TSL on poly-mineral and etched samples. Luminescence dating and profiling indicates that the upper archaeological layer (SU-1B probably dates to the Late Bronze Age (ca. 1000 BC, but that the sediments in the remainder of this section accumulated during the late Neolithic-Copper Age: the site was most probably inhabited between 3300 and 2900 BC. There were gaps in occupation between the Late Neolithic-Cooper Age phases (I & II, and between the Late Neolithic-Cooper Age phases and Late Bronze Age phase (II & III, indicating that the site was abandoned and reoccupied twice. The AMS-14C date on deer antler of 4254 ± 45 BP (3010-2675 cal BC is consisten with the OSL results for phase II, indicating that the pit was excavated at the beginning of the second occupational phase. Combined OSL

  14. The Trade City of Kulum in Qing Dynasty%清代库伦的买卖城

    Institute of Scientific and Technical Information of China (English)

    赖惠敏

    2015-01-01

    库伦的买卖城可能建于雍正五年(1727)年,中俄签订《恰克图条约》后。当时,库伦买卖城做为囤积货物之地,以张家口的商号为总号,在库伦或恰克图设立分号。晋商与俄罗斯的贸易,19世纪茶叶贸易活络,最高贸易额曾达1500万卢布,每年有7万只骆驼往来于库伦与张家口之间。但1860年签订《中俄北京条约》,俄商到中国贸易以及西伯利亚铁路建成后,晋商的茶叶贸易急遽下降,少数商人维持与俄贸易,他们向俄国人购买鹿茸、皮张,然后向蒙古人购买羊只,转卖给俄商。资金雄厚的商号转而经营金融业或批发百货,如公合全、协和公、双舜全、兴隆魁等。同时,有商铺如林盛元、义合忠、天泰昌等从事农垦或砍伐木材活动。本文主要讨论清中叶以后买卖城商号的转变。%The Trade City of Kulum was probably built in 1727 ,after Kyakhta treaty was signed .At that time ,the Trade City of Kulum was set mainly for a place of stocking goods .With headquarter in Zhangjiakou ,many shops set up their branch offices in Kulum or Kyakhta .T he trading that Shanxi mer‐chants and Russian had was very active during nineteen the century .The peak trading amount once reached to 15 million rubles .There were seventy thousand camels going back and forth between Kulum and Zhangjiakou .After Sino‐Russian Treaty of Beijing was signed in 1860 ,Russian merchants came to China for trading and the Riyadh Siberian Railway completed ,and tea trading of Shanxi merchants dropped sharply .Only a few merchants kept the business with Russian traders .They brought antler ,hides from Russian ,then brought goats/sheep to sell to Russian .Some shops with strong capital turned to do finan‐cial business or wholesalers ,like Gong He Quan ,Xie He Gong ,Shuang Shun Quan ,Xing Long Kui ,etc . Meanwhile some carried on land reclamation and felling timers ,like Lin Shen Yuan ,Yi Ho

  15. Food science in developing countries.

    Science.gov (United States)

    Brown, N L; Pariser, E R

    1975-05-01

    It is particularly important for us not to lose sight of the fact that people have been around for a long time and that they achieved remarkable technical skills long before Western science was developed. An anonymous writer from the Food and Agriculture Organization has observed: "It is a commonplace that the fundamental discoveries which made civilization possible-fire- making, tool-making, agriculture, building, calculating, writing, money-were all apparently made outside the area which has given us the marvels of modern science" (19). The writer might well have added that it is also commonly overlooked that food technology was not suddenly developed in the 20th century but has been very much a part of the lives of people everywhere ever since they began doing more to their food than gathering it and eating it raw. Lamb's "Essay on Roast Pig" may not be an accurate account of the first conjunction of fire and food, but cooking is a rather ancient practice. Fermentation is another complicated processing technology which is a traditional part of most cultures, particularly those in warm climates-beer, yogurt, cheese, the fish pastes and sauces of Asia, the palm wine of Africa, and soy sauce, are butsome examples. Native Americans, besides accomplishing marvels in plant genetics and crop development, also developed water extraction methods for treating acorns to render the flour palatable and edible, and the alkali method of processing maize. Furthermore, they developed a cure for scurvy-by making a water extraction of pine needles which are rich in ascorbic acid-long before it was first reported by Jacques Cartier in the 16th century. Similarly, calcium-deficient diets of pregnant and nursing women were traditionally successfully supplemented by calcium-rich powdered deer antlers in northern China. Among the Chinese and Greeks, goiter was cured by eating certain kinds of seaweed centuries before the disease was traced to a lack of iodine, and Kenyans learned to

  16. Rainwater Wildlife Area Management Plan Executive Summary : A Columbia Basin Wildlife Mitigation Project.

    Energy Technology Data Exchange (ETDEWEB)

    Childs, Allen B.

    2002-02-01

    access. During the past two years, non-Indian public concern over big game hunting issues has at times overwhelmed other issues related to the wildlife area. In 2001, the CTUIR Fish and Wildlife Committee closed the wildlife area to tribal branch antlered bull elk harvest in response to harvest data that indicated harvest rates were greater than expected. In addition, illegal harvest of mature bull elk in southeastern Washington during the 2001 season exceeded the legal tribal and nontribal harvest combined which has created a potential significant regression in the bull;cow ratio in the Blue Mountain Elk herd. CTUIR Fish and Wildlife Committee and staff and Washington Department of Fish and Wildlife Regional Director and staff have been coordinating regularly to develop strategies to address harvest rates and ensure protection of viable big game herds in southeastern Washington. The CTUIR Fish and Wildlife Committee and WDFW has jointly agreed to continue close coordination on this and other issues and continue working together to ensure the long-term vigor of the elk herd on the Rainwater Wildlife Area. The purpose of the project is to protect, enhance, and mitigate fish and wildlife resources impacted by Columbia River Basin hydroelectric development. The effort is one of several wildlife mitigation projects in the region developed to compensate for terrestrial habitat losses resulting from the construction of McNary and John Day Hydroelectric facilities located on the mainstem Columbia River. While this project is driven primarily by the purpose and need to mitigate for wildlife habitat losses, it is also recognized that management strategies will also benefit many other non-target fish and wildlife species and associated natural resources.

  17. 麇鹿角醇提液对环磷酰胺致雄鼠性腺损伤的保护作用

    Institute of Scientific and Technical Information of China (English)

    成海龙; 陈鹤林; 韩中保; 田郡; 陆晓东; 秦红兵

    2011-01-01

    目的:观察麋鹿角醇提液对环磷酰胺所致雄性小鼠性腺损伤的保护作用.方法:应用腹腔注射环磷酰胺造成小鼠性腺抑制模型,给予麋鹿角醇提液不同剂量进行干预,然后测定小鼠血清中的睾酮以及黄体生成素的水平,检测睾丸以及副性器官质量指数、精子密度和精子活动率,检测睾丸组织中超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malonaldehyde,MDA)含量,光镜检测睾丸组织形态学变化.结果:麇鹿角醇提液能良好对抗环磷酰胺引起的睾酮与黄体生成素下降,能提高模型睾丸质量指数,提高模型精子密度与精子活率,增加模型组睾丸SOD活力,并减少其MDA含量,改善环磷酰胺所致模型小鼠睾丸组织的病理性变化.结论:麋鹿角醇提液对环磷酰胺致雄鼠性腺损伤具有保护作用,可能与其促性激素样作用以及抗氧化作用有关.%Objective:To observe the protective effect of Elk antlers ethanolic fluidextract (EAEF) on gonad function impairment of male mice induced by cyclophosphamide (CP). Methods;The model of gonad function depression was prepared by repeatedly in-traperitoneal injection of CP. Low and high dose of EAEF were given to mice with oral administration to intervene this process,then the level of testosterone and luteinizing hormone in serum were detected;the weight index of sex organ and accessory sex organ were observed;sperm density and sperm activity were detected;the superoxide dismutase (SOD) activity and malonaldehyde(MDA) content were measured. Meanwhile the histopathological changes in testis were observed by light microscopy. Results;EAEF reversed the decline of testosterone and luteinizing hormone level in serum,enhanced the testicle mass index,sperm density and sperm activity, increased SOD activity and decreased content of MDA in testis,as well alleviated the histologic pathological formation in testis in cyclophosphamide-induced model mice. Conclusion

  18. Reconsideration of the systematics of the Early Pleistocene Cervavitus (Cervidae, Artiodactyla, Mammalia

    Directory of Open Access Journals (Sweden)

    Dong, W.

    2011-12-01

    Full Text Available Cervavitus were usually found from the Late Miocene and Pliocene deposits in East Europe, Middle Asia and North China, but they were found recently in many Early Pleistocene localities in southern China. The latter resulted in the discussion of their systematic status between Cervavitus and Cervus. Here we show the Early Pleistocene forms from southern China are morphometrically more similar to northern China Cervavitus species, and the cladistic analysis shows that the southern China forms are closer to classic Cervavitus species than Cervus and that also proves their systematic status in Cervavitus rather than in Cervus. Cervavitus originated in Moldovan forests of East Europe in the late Vallesian (MN10 from a brachyodont and holometacarpal ancestor with two/three-tined antlers and Palaeomeryx fold and evolved into C. novorossiae. It dispersed into West Europe forests in the earliest Turolian and further west to France in the Ruscinian. It dispersed into northern China forests in the early Turolian and represented by C. shanxius. The great quantity of C. shanxius specimens with brachyodont teeth and complete lateral metacarpals implies the arid Loess Plateau of today was a humid forested region in the Late Miocene. C. shanxius migrated southwards in the Plio-Pleistocene probably due to the drying environment in northern China with uplifting of Himalayas and evolved into C. ultimus and C. fenqii, which survived in southern China until the Early Pleistocene (MNQ18.La revisión sistemática de Cervivatus sugiere que deriva del principal clado de los cérvidos posteriores a los muntiacinos, e implica que Procervulinae, Dicrocerinae y la primeras formas de Munticiacinae serían holometacarpales, como también lo es Cervivatus, originario en los bosques de Moldavia (Europa del Este durante el Vallesiense final (MN 10, a partir de un antecesor braquiodonto y holometacarpal, con astas con dos o tres candiles y pliegue paleomerícido, y que da lugar a

  19. THE FIRST TRIAL EXCAVATION AND SIGNIFICANCE OF WULANMULUN SITE IN 2010 AT ORDOS,INNER MONGOLIA IN NORTH CHINA%内蒙古鄂尔多斯乌兰木伦遗址2010年1期试掘及其意义

    Institute of Scientific and Technical Information of China (English)

    侯亚梅; 刘扬; 白林云; 包蕾; 李双; 杨俊刚; 张立民; 张智杰; 王志浩; 杨泽蒙; 甄自明; 张家富; 董为; 袁宝印; 李保生; 黄慰文

    2012-01-01

    The Wulanmulun Site was discovered at left bank of Wulamulun River in Ordos, Inner Mongolia Autonomous Region in North China with location of 39°35. 152'N, 109°45. 659'E and 1281m above sea level. The first period trial excavation was carried out in early summer of 2010. The main part of deposition were divided into 8 layers with depth of about 5 ~ 8m. Among them seven archaeological layers are recognized according to their existence of geological deposition, mammalian fauna and stone artifacts remains.More than 1500 stone artifacts were yielded and appeared general types of core,flake and major flake tools. Single platform core are very popular. Used flakes were obviously needed with longer and rather straight edge accompanied by natural or backed back. Prepared platform can be observed on few numbers of flakes. Lot of debris by flaking on core or retouching work were fund from excavation. Retouched pieces are mostly made on flakes. There are different types of scrapers like all kinds of concave, convex, straight and concave-convex. Denticulate and notch were very much seen and even made into subtypes. Borer, drill, and various types of points were frequently appeared out. End-scraper, bec and burin were sometimes observed. Multiple functional phenomenon are common on one piece of tool. In summary, the lithic assemblage belongs to flake tool and share some similarity with characters of Middle Paleolithic of European Mousterian tradition.Totally 1213 pieces of mammalian bones were obtained from seven archaeological layers. Based on the numbers of unearthed fossil specimens of Wulanmulun fauna, the wooly rhinos are the most numerous species, followed by Przewalsky's horse, and then Ordos' giant antlered deer. The other taxa are not numerous. Judging by the condition of deciduous and permanent teeth, the juvenile wooly rhinos are more numerous than adult ones. The other taxa are mostly dominated by adult specimens or difficult to judge due to fragmentary and

  20. Renal Parenchyma Lithotomy by Hypothermic Renal Vascular Block%低温下原位肾动脉阻断治疗复杂性肾结石

    Institute of Scientific and Technical Information of China (English)

    乙从亮; 杨登伦; 任春凯; 朱巍; 余秋健; 陈令秋

    2014-01-01

    目的:探讨开放性手术低温条件下采用肾动脉阻断治疗复杂肾结石患者的方法以及临床疗效。方法:对32例复杂性肾结石患者采用静脉注射肌苷2.0 g,原位低温下阻断肾动脉后行肾实质切开取石术。术前经CT、KUB、IVP等检查诊断鹿角形结石或(和)多发结石。平均年龄48(19~65)岁。左肾24例,右肾8例。病史6个月~20年。结石最大直径2.5~7.0 cm,平均为3.5 cm。,术后随访。结果:本组均采用原位低温肾动脉阻断肾实质切开取石术。血流阻断时间15~40分钟,平均25分钟,术中出血量80~300 ml,平均180ml,肾实质切口大小3~5 cm,平均3.5cm,手术操作时间90~150分钟,平均110分钟。术中无大出血及血管损伤。术后并发大出血1例,再次手术缝合止血顺利出院,3例术后肉眼血尿2~3个月,经保守治疗后治愈,无并发症。结论:小切口原位低温下阻断肾动脉配合静脉注射肌苷行肾实质切开取石手术具有方法容易掌握、出血少、创伤小、肾功能损害小、手术安全等特点,是治疗复杂性肾结石安全、有效的方法之一,易于在无条件行微创手术的医院开展。%Objective:Explore the open surgery by renal artery block under the condition of low temperature treatment of complex method and clinical efficacy in patients with kidney stones .Methods:32 cases of patients with renal stones complexity by intravenous in-jection of inosine 2.0 g, block the renal artery in situ low temperature renal parenchyma incision after nephrolithotomy .Preoperative CT, the KUB, IVP, such as inspection diagnosis antlers or (and) multiple stones.The average age is 48 (19-65).The left kidney was 24 cases, 8 cases of right kidney .History was 6 months to 20 years.The stone diameter is from 2.5 to 7.0 cm, with an average of 3.5 cm. Results:All use of in situ cryogenic renal artery block the renal parenchyma