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Sample records for antiseptics

  1. Quantitative evaluation of dermatological antiseptics.

    Science.gov (United States)

    Leitch, C S; Leitch, A E; Tidman, M J

    2015-12-01

    Topical antiseptics are frequently used in dermatological management, yet evidence for the efficacy of traditional generic formulations is often largely anecdotal. We tested the in vitro bactericidal activity of four commonly used topical antiseptics against Staphylococcus aureus, using a modified version of the European Standard EN 1276, a quantitative suspension test for evaluation of the bactericidal activity of chemical disinfectants and antiseptics. To meet the standard for antiseptic effectiveness of EN 1276, at least a 5 log10 reduction in bacterial count within 5 minutes of exposure is required. While 1% benzalkonium chloride and 6% hydrogen peroxide both achieved a 5 log10 reduction in S. aureus count, neither 2% aqueous eosin nor 1 : 10 000 potassium permanganate showed significant bactericidal activity compared with control at exposure periods of up to 1 h. Aqueous eosin and potassium permanganate may have desirable astringent properties, but these results suggest they lack effective antiseptic activity, at least against S. aureus.

  2. Quantitative evaluation of dermatological antiseptics.

    Science.gov (United States)

    Leitch, C S; Leitch, A E; Tidman, M J

    2015-12-01

    Topical antiseptics are frequently used in dermatological management, yet evidence for the efficacy of traditional generic formulations is often largely anecdotal. We tested the in vitro bactericidal activity of four commonly used topical antiseptics against Staphylococcus aureus, using a modified version of the European Standard EN 1276, a quantitative suspension test for evaluation of the bactericidal activity of chemical disinfectants and antiseptics. To meet the standard for antiseptic effectiveness of EN 1276, at least a 5 log10 reduction in bacterial count within 5 minutes of exposure is required. While 1% benzalkonium chloride and 6% hydrogen peroxide both achieved a 5 log10 reduction in S. aureus count, neither 2% aqueous eosin nor 1 : 10 000 potassium permanganate showed significant bactericidal activity compared with control at exposure periods of up to 1 h. Aqueous eosin and potassium permanganate may have desirable astringent properties, but these results suggest they lack effective antiseptic activity, at least against S. aureus. PMID:26456933

  3. [Hexetidine--an oral antiseptic].

    Science.gov (United States)

    Kapić, Elvedina; Becić, Fahir; Becić, Ervina

    2002-01-01

    Hexetidine is very safe oral antiseptic with broad antibacterial and antifungal activity in vivo and in vitro. It has local-anesthetics, astringent and deodorant activity. Also, it has very strong antiplac effects. Resistention of microorganisms on hexetidine is short and transient. These characteristics give important therapeutic role in treatment of oral infections.

  4. Pre-operative antiseptic skin preparation.

    Science.gov (United States)

    Murkin, Claire Elizabeth

    Theatre nurses use antiseptic skin preparation products every day, but little thought seems to be given as to why a surgeon has a particular preference for one antiseptic skin preparation over another - whether it is for its efficacy, safety or application properties. Woodhead et al (2004) states that nurses still work in a ritualistic environment. Rituals are 'any action performed according to custom, without understanding the reasons why it is being practised'. Nursing practice should be evidence-based; nurses should understand the rationale behind the choice of a particular antiseptic, and be knowledgable about the clinical effectiveness of antiseptic's use pre-operatively, to achieve optimum results. This article focuses on the main types of antiseptic skin preparation while highlighting each product's activity and the relevant considerations for choosing the appropriate product for each patient. Theatre staff need to emphasize the importance of skin preparation and the correct application techniques, while educating the scrub team and surgeons with respect to skin preparation.

  5. Novel water-based antiseptic lotion demonstrates rapid, broad-spectrum kill compared with alcohol antiseptic.

    Science.gov (United States)

    Czerwinski, Steven E; Cozean, Jesse; Cozean, Colette

    2014-01-01

    A novel alcohol-based antiseptic and a novel water-based antiseptic lotion, both with a synergistic combination of antimicrobial ingredients containing 0.2% benzethonium chloride, were evaluated using the standard time-kill method against 25 FDA-specified challenge microorganisms. The purpose of the testing was to determine whether a non-alcohol product could have equivalent rapid and broad-spectrum kill to a traditional alcohol sanitizer. Both the alcohol- and water-based products showed rapid and broad-spectrum antimicrobial activity. The average 15-s kill was 99.999% of the challenge organism for the alcohol-based antiseptic and 99.971% for the water-based antiseptic. The alcohol-based product demonstrated 100% of peak efficacy (60s) within the first 15s, whereas the water-based product showed 99.97%. The novel alcohol-based antiseptic reduced concentrations of 100% of organisms by 99.999%, whereas the water-based antiseptic lotion showed the same reduction for 96% of organisms. A novel water-based antiseptic product demonstrated equivalent rapid, broad-spectrum antimicrobial activity to an alcohol-based sanitizer and provided additional benefits of reduced irritation, persistent effect, and greater efficacy against common viruses. The combination of rapid, broad-spectrum immediate kill and persistent efficacy against pathogens may have significant clinical benefit in limiting the spread of disease.

  6. Antibiotics and antiseptics for pressure ulcers

    OpenAIRE

    Norman, G.

    2015-01-01

    This is the protocol for a review and there is no abstract. The objectives are as follows: To assess the effects of systemic and topical antimicrobials, and topical antiseptics on the healing of infected and uninfected pressure ulcers being treated in any clinical setting.

  7. In vitro antiplaque effects of antiseptic phenols.

    Science.gov (United States)

    Evans, R T; Baker, P J; Coburn, R A; Fischman, S L; Genco, R J

    1977-03-01

    Several phenols known to be antiseptics were tested in vitro for their ability to adsorb to saliva-coated enamel and subsequently inhibit plaque formation. 3,5,4'-tribromosalicylanilide was found to be effective against growth and plaque formation of A viscosus, A naeslundii, S mutans and S sanguis. Dibromsalicil was effective against A viscosus. The other phenols (hexylresorcinol, thymol, phenylphenol and zinc phenolsulfonate) did not inhibit in vitro growth or plaque formation.

  8. A review of antiseptics. Cleansing agents.

    Science.gov (United States)

    Leclair, J

    1990-10-01

    1. The cells of the stratum corneum are rough, jagged, and contain myriad niches in which bacteria dwell. The complex structure of the stratum corneum limits complete access of any chemical; an antiseptic agent must have contact to kill bacteria. 2. Shaving hair on the skin, long considered essential prior to surgery, has been shown to sabotage the effectiveness of the preoperative skin preparation. 3. Experimental evidence over a long period has documented that no other agent can achieve as rapid and large a reduction in skin flora as alcohol. 4. The preoperative hand scrub calls not only for removal of dirt, grease, and pathogens from the surface of the hands, but also a maximum reduction of the resident flora.

  9. Current and new strategies for the delivery of antiseptic agents.

    Science.gov (United States)

    Constant, Hélène; Falson, Françoise; Pirot, Fabrice

    2006-07-01

    The present mini-review explores the current methods used for the delivery of antiseptics and topical antimicrobials. Relevance of hand scrub with antiseptic liquid soap (e.g. chlorhexidine, PVP-iodine, triclosan) and alcohol-based hand rub is discussed and compared in terms of bactericidal activity, skin tolerance, and medical staff observance. New strategies for antibacterial delivery focus on the challenge of colloidal drug carrier such as liposomes, micro- and nanoparticles enabling sustained bactericidal effect and effective bacterial targeting.

  10. Experimental evaluation of antifungal and antiseptic agents against Rhodotorula spp.

    Science.gov (United States)

    Preney, L; Théraud, M; Guiguen, C; Gangneux, J P

    2003-12-01

    We studied the susceptibility of 21 strains of Rhodotorula rubra and nine strains of R. glutinis to eight antifungals and tested eight antiseptic agents on one strain of R. rubra. The tested strains were susceptible to ketoconazole, 5-fluorocytosine, amphotericin B, and nystatin, intermediate to econazole and resistant to fluconazole, itraconazole and miconazole. After 5-min contact, six of the eight antiseptic agents tested showed a fungicidal activity on the tested R. rubra strain.

  11. [Antiseptics in neonatology: the inheritance of the past in the daylight].

    Science.gov (United States)

    Lahmiti, S; El Fakiri, K; Aboussad, A

    2010-01-01

    The antiseptics are antimicrobial substances that are applied to living tissue to reduce the growth of microorganisms. The physiological particularities of newborns, and especially premature, reduce the list of used antiseptic in neonatology. The choice of antiseptic in neonates depends on its activity, its presentation and its tolerance. Using a large spectrum antiseptic like iodine and chlorhexidin seems to be the best choice; however the thyroid toxicity of iodine products contraindicates their use in the newborn. The usage of minor antiseptics like hexamidin shows no interest due to their limited spectrum and their long action delay. Using eosin for umbilical cord care is not recommended because it doesn't have any antiseptic activity and causes neonatal infections. Through this article, the authors review the principal aspects of antiseptic and report the properties and advantages-disadvantages of each antiseptic. The goal is to facilitate the choice of antiseptic used in neonatology.

  12. Toxicity of antiseptics against chondrocytes: What is best for the cartilage in septic joint surgery?

    OpenAIRE

    Röhner, Eric; Kolar, Paula; Seeger, Joern B.; Arnholdt, Joerg; Thiele, Kathi; Perka, Carsten; Matziolis, Georg

    2011-01-01

    In septic joint surgery, the most frequently used antiseptics are polyhexanide, hydrogen peroxide and taurolidine. The aim of this study was to examine the effects of these antiseptics on viability of human chondrocytes. Our hypothesis was that antiseptics and supplemental irrigation with sodium chloride lavage are less toxic on human chondrocytes than treatment with antiseptics only. Primary human chondrocytes were isolated and cultured from six donated human knee joints. Polyhexanide, hydro...

  13. 78 FR 76443 - Safety and Effectiveness of Consumer Antiseptics; Topical Antimicrobial Drug Products for Over...

    Science.gov (United States)

    2013-12-17

    ... active ingredients were classified for OTC antiseptic handwash use (59 FR 31402 at 31435) (for a list of... TFM covering the first aid antiseptics in the Federal Register of July 22, 1991 (56 FR 33644) (First... requested relevant data and information (59 FR 31402 at 31440). Antiseptics for use by the food industry...

  14. Fluorescence Assay for Evaluating Microbicidal Activity of Hand Antiseptics

    OpenAIRE

    Lopez-Gigosos, Rosa M.; Mariscal, Alberto; Mariscal-Lopez, Eloisa; Gutierrez-Bedmar, Mario; Fernandez, Joaquin

    2015-01-01

    We developed a fluorescent β-d-glucuronidase activity (BGA)-based assay for detecting and quantifying Escherichia coli in samples to assess the biocide efficacy of hand antiseptics. The fluorescence level is proportional to the number of viable E. coli organisms present. We compared our assay results to those of the E. coli plate count method specified by the European standard for testing hygienic hand rub disinfectant products (EN1500). The plate count method requires excessive handling and ...

  15. ANTIMICROBIAL, PHYSICAL AND CHEMICAL QUALITIES OF MEDICINAL ANTISEPTIC DRUGS

    Directory of Open Access Journals (Sweden)

    Paliy D. V.

    2014-12-01

    Full Text Available In our research results of the study of antimicrobial, physical and chemical qualities of antiseptic medicines of decamethoxin (DCM. Antimicrobial activity of DCM, palisan, decasan, deseptol against srains of S.aureus (n 56, S.epidermidis (n 26, E.coli (n 24, P.mirabilis (n 11, P.vulgaris (n 8 was studied by means of method of serial dilutions. Obtained data of mass spectrometry study of antimicrobial compositions with constant concentrations of DCM have shown that medicinal forms of DCM are complex physical and chemical systems, because of different origin and number of adjuvant ingredients used during their fabrication. Among synthetic quaternary ammonium agents there have been found the substance (commercial name of medicine is decamethoxin to have high antimicrobial activity against strains of grampositive and gram-negative microorganisms, an also C.albicans. There was found that antimicrobial activity of antiseptic palisan had been higher comparably to DCM in equivalent concentration. The composition and concentrations of acting agents and the methodology of preparation of palisan have been substantiated on the basis of microbiological, mass spectrometry characteristics of antiseptics DCM, palisan.

  16. Correlation of the antiseptic resistance in Acinetobacter baumannii and the antiseptic resistance gene qacE△ 1 located in class Ⅰ integron

    Institute of Scientific and Technical Information of China (English)

    WEI FENG SHI; JIAN PING QIN; NING XU

    2006-01-01

    In the past decade, uses of antiseptics and disinfectants in hospitals and other health care centers are rather common, but the chance to develop resistance to antiseptics and disinfectants is also increased. Acinetobacter baumannii is one of the opportunistic bacteria involving in the nosocomial infection. In the present study, the correlation of the antiseptic resistance in A. baumannii and the antiseptic resistance gene qacE△1 was investigated by means of determination of MICs. Meanwhile, the MICs of glutaraldehyde, chlorhexidine, benzalkonium bromide, iodophor and trichloroisocyanurate to 80 clinical isolates of A. baumannii were detected by tube dilution assay and the resistance genes intI1 and qacE △ 1 in these isolates were amplified by PCR and verified by DNA sequencer. It was found that the MIC50 for these 5 antiseptics tested were 32, 8, 8, 4 and 1 μg/ml respectively, and the detection rates of intI1 and qacE△1 gene were 60.0% and 77.6% respectively. In addition, 55% of the 80 isolates simultaneously possessed both intI1 and qacE△ 1 gene, and the percentage of antiseptic resistance of A. baumannii carring both genes to benzalkonium bromide were higher than that without these two genes, however, there was no significant difference between intI1 and qacE△1 gene. The result in bactericidal efficiency assay indicated that chlorhexidine could still produce rapid and strong bactericidal effect at concentration of 1 MIC after 10 min exposure. These results suggest that the antiseptic resistance of A. baumannii to various antiseptics is correlated with the presence of the antiseptic resistance genes qacE△1 in bacteria, thus warning that the increase of the antiseptic resistance should not be ignored and the relative high concentration or prolonged application time is required to achieve a sufficient bactericidal effect.

  17. Controlled antiseptic release by alginate polymer films and beads.

    Science.gov (United States)

    Liakos, Ioannis; Rizzello, Loris; Bayer, Ilker S; Pompa, Pier Paolo; Cingolani, Roberto; Athanassiou, Athanassia

    2013-01-30

    Biodegradable polymeric materials based on blending aqueous dispersions of natural polymer sodium alginate (NaAlg) and povidone iodine (PVPI) complex, which allow controlled antiseptic release, are presented. The developed materials are either free standing NaAlg films or Ca(2+)-cross-linked alginate beads, which properly combined with PVPI demonstrate antibacterial and antifungal activity, suitable for therapeutic applications, such as wound dressing. Glycerol was used as the plasticizing agent. Film morphology was studied by optical and atomic force microscopy. It was found that PVPI complex forms well dispersed circular micro-domains within the NaAlg matrix. The beads were fabricated by drop-wise immersion of NaAlg/PVPI/glycerol solutions into aqueous calcium chloride solutions to form calcium alginate beads encapsulating PVPI solution (CaAlg/PVPI). Controlled release of PVPI was possible when the composite films and beads were brought into direct contact with water or with moist media. Bactericidal and fungicidal properties of the materials were tested against Escherichia coli bacteria and Candida albicans fungi. The results indicated very efficient antibacterial and antifungal activity within 48 h. Controlled release of PVPI into open wounds is highly desired in clinical applications to avoid toxic doses of iodine absorption by the wound. A wide variety of applications are envisioned such as external and internal wound dressings with controlled antiseptic release, hygienic and protective packaging films for medical devices, and polymer beads as water disinfectants.

  18. Antiseptics and microcosm biofilm formation on titanium surfaces

    Directory of Open Access Journals (Sweden)

    Georgia VERARDI

    2016-01-01

    Full Text Available Abstract Oral rehabilitation with osseointegrated implants is a way to restore esthetics and masticatory function in edentulous patients, but bacterial colonization around the implants may lead to mucositis or peri-implantitis and consequent implant loss. Peri-implantitis is the main complication of oral rehabilitation with dental implants and, therefore, it is necessary to take into account the potential effects of antiseptics such as chlorhexidine (CHX, chloramine T (CHT, triclosan (TRI, and essential oils (EO on bacterial adhesion and on biofilm formation. To assess the action of these substances, we used the microcosm technique, in which the oral environment and periodontal conditions are simulated in vitro on titanium discs with different surface treatments (smooth surface - SS, acid-etched smooth surface - AESS, sand-blasted surface - SBS, and sand-blasted and acid-etched surface - SBAES. Roughness measurements yielded the following results: SS: 0.47 µm, AESS: 0.43 µm, SB: 0.79 µm, and SBAES: 0.72 µm. There was statistical difference only between SBS and AESS. There was no statistical difference among antiseptic treatments. However, EO and CHT showed lower bacterial counts compared with the saline solution treatment (control group. Thus, the current gold standard (CHX did not outperform CHT and EO, which were efficient in reducing the biofilm biomass compared with saline solution.

  19. N-chlorotaurine, a natural antiseptic with outstanding tolerability.

    Science.gov (United States)

    Gottardi, Waldemar; Nagl, Markus

    2010-03-01

    N-chlorotaurine, the N-chloro derivative of the amino acid taurine, is a long-lived oxidant produced by activated human granulocytes and monocytes. Supported by a high number of in vitro studies, it has mainly anti-inflammatory properties and seems to be involved in the termination of inflammation. The successful synthesis of the crystalline sodium salt (Cl-HN-CH(2)-CH(2)-SO(3)Na, NCT) facilitated its development as an endogenous antiseptic. NCT can be stored long-term at low temperatures, and it has killing activity against bacteria, fungi, viruses and parasites. Transfer of the active chlorine to amino groups of molecules of both the pathogens and the human body (transhalogenation) enhances rather than decreases its activity, mainly because of the formation of monochloramine. Furthermore, surface chlorination after sublethal incubation times in NCT leads to a post-antibiotic effect and loss of virulence of pathogens, as demonstrated for bacteria and yeasts. Being a mild oxidant, NCT proved to be very well tolerated by human tissue in Phase I and II clinical studies. A 1% aqueous solution can be applied to the eye, skin ulcerations, outer ear canal, nasal and paranasal sinuses, oral cavity and urinary bladder, and can probably be used for inhalation. Therapeutic efficacy in Phase II studies has been shown in external otitis, purulently coated crural ulcerations and keratoconjunctivitis, so far. Based upon all presently available data, NCT seems to be an antiseptic with a very good relation between tolerability and activity. Recently, C-methylated derivatives of NCT have been invented, which are of interest because of improved stability at room temperature.

  20. [Antibacterial activity of antiseptics used at Military Teaching Hospital Mohamed V of Rabat].

    Science.gov (United States)

    Essayagh, Touria; Elameri, Abdouelouhab; Zohoun, Alban; Miloudi, Mouhcine; Elhamzaoui, Sakina

    2010-01-01

    Antiseptics have a major role against the infections and their prevention. The good management of antiseptics allows the reduction of antibiotics use and thus the emergence of resistant bacterial strains. The evaluation of the antibacterial activity of three antiseptics (povidone iodine [PVPI], iodized alcohol and alcohol 70 degrees) used at HMIMV and taken from pharmacy was based on AFNOR method NF T 72-150. The analysis of their chemical properties were done by standardized methods (manganimetry, Bunsen's method, test to determine sodium thiosulfate levels [or sodium thiosulfate test] and Guy Lussac alcoholmeter). Our results were compared with those obtained in another two university hospitals of Rabat: Hospital of Speciality and Ibn Sina. The frequencies of resistant bacterial strains were respectively 4.6%, 30.7% and 15.4% to PVPI, alcohol iodized and alcohol 70 degrees . Our results have shown that the PVPI is the best antiseptic in our hospital.

  1. Design of antiseptic formulations containing extract of Plinia cauliflora

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    Lara Alexandre de Oliveira

    2011-09-01

    Full Text Available The leaves of the Brazilian species Plinia cauliflora were used to obtain active hydroalcoholic extract and fractions enabling the development of efficient antiseptic pharmaceutical formulations. A chemical composition of 70% ethanol extract, aqueous and ethyl acetate fractions was analyzed by thin-layer chromatography and for phenol content. Antimicrobial activity was tested against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Lactobacillus acidophilus and Candida albicans by the agar diffusion method and the minimum inhibitory concentration was assayed by broth microdilution. Extract microbiological quality was tested to avoid contamination in the formulations. A mouthwash and a topical cream containing the extract were developed and antiseptic activity was assessed by agar diffusion. Sensory and physicochemical stability of the formulations were assayed. Chromatography indicated the presence of terpenes, flavonoids and tannins in the extract and fractions and total phenol content were found to be high. The plant samples were active against all the microorganisms tested, except for Lactobacillus acidophilus. Both topical formulations showed antiseptic activity and stability. Thus, these may be used as antimicrobials in skin infections, but would be more useful in the treatment of candidiasis.As folhas da espécie brasileira Plinia cauliflora foram utilizadas a fim de se obter um extrato hidroalcoólico e frações ativas proporcionando o desenvolvimento de eficazes formulações farmacêuticas antissépticas. A composição química do extrato etanólico 70%, fração aquosa e acetato de etila foi analisada por cromatografia em camada delgada e teor de fenóis. A atividade antimicrobiana foi testada frente a Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Lactobacillus acidophilus e Candida albicans por difusão em ágar e a concentração inibitória mínima foi determinada por microdiluição. A

  2. Antibacterial effect of oral antiseptics on facultative bacteria

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    Marcel da Silva Garrote

    2013-01-01

    Full Text Available Purpose: To evaluate the antibacterial effect of four oral antiseptics (two solutions of cetylpyridinium chloride, chlorhexidine gluconate and benzalkonium chloride on facultative bacteria using two methods. Methods: Strains were inoculated in 7 mL of brain heart infusion (BHI and incubated at 37°C for 24 hours. For the agar diffusion test, 15 Petri plates with 20 mL of brain heart infusion agar (BHIA were inoculated with 0.1 mL of microbial suspensions using sterile swabs to produce confluent growth; one Petri plate was not inoculated. Thirty-six 9-mm paper discs were immersed in the experimental solutions (0.07% cetylpyridinium chloride, 0.075% cetylpyridinium chloride, 0.12% chlorhexidine gluconate, and 0.13% benzalkonium chloride for 1 minute. Subsequently, three paper discs containing irrigant solutions were placed on the BHIA in each plate. The plates were kept at room temperature for 1 hour and incubated at 37°C for 48 hours. Two measurements of the inhibition zones were made on the paper discs containing the solutions, and mean values were calculated. For the direct exposure test, 216 #50 sterilized paper points were immersed in the microorganism suspensions for 5 minutes, placed onto Petri plates and covered with 10 mL of irrigant solution. At one, five, 10 and 30 minutes, three paper points were removed from the contact substances, transported individually, immersed in 7 mL Letheen broth and incubated at 37°C for 48 hours. Bacterial growth was evaluated by turbidity. An inoculum of 0.1 mL Letheen broth was transferred to 7 mL BHI, and incubated as described above. Bacterial growth was evaluated according to turbidity. Results: Inhibition zones were greater than 10 mm for all substances and all microorganisms under study. The antibacterial effect of 0.13% benzalkonium chloride against the biological indicators was observed after five minutes in direct exposure, while cetylpyridinium chloride and chlorhexidine gluconate had an

  3. Evaluation of antiseptic antiviral activity of chemical agents.

    Science.gov (United States)

    Geller, Chloé; Finance, Chantal; Duval, Raphaël Emmanuel

    2011-06-01

    Antiviral antisepsis and disinfection are crucial for preventing the environmental spread of viral infections. Emerging viruses and associated diseases, as well as nosocomial viral infections, have become a real issue in medical fields, and there are very few efficient and specific treatments available to fight most of these infections. Another issue is the potential environmental resistance and spread of viral particles. Therefore, it is essential to properly evaluate the efficacy of antiseptics-disinfectants (ATS-D) on viruses. ATS-D antiviral activity is evaluated by (1) combining viruses and test product for an appropriately defined and precise contact time, (2) neutralizing product activity, and (3) estimating the loss of viral infectivity. A germicide can be considered to have an efficient ATS-D antiviral activity if it induces a >3 or >4 log(10) reduction (American and European regulatory agency requirements, respectively) in viral titers in a defined contact time. This unit describes a global methodology for evaluating chemical ATS-D antiviral activity.

  4. Activity of the antiseptic polyhexanide against gram-negative bacteria.

    Science.gov (United States)

    Fabry, Werner Hugo Karl; Kock, Hans-Jürgen; Vahlensieck, Winfried

    2014-04-01

    The activity of the antiseptic polyhexanide was tested against 250 gram-negative clinical isolates, that is, 50 isolates each of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Moraxella catarrhalis, and Haemophilus influenzae. Minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were determined by using a serial broth microdilution technique according to DIN 58940. Time-kill studies were performed for reference stains E. coli ATCC 25922, K. pneumoniae ATCC 4352, P. aeruginosa ATCC 15442, M. catarrhalis ATCC 43617, and H. influenzae ATCC 49247. All tested isolates had MICs and MBCs within a range of 1-32 mg/L and were regarded as susceptible to polyhexanide. The highest values were found for P. aeruginosa and H. influenzae with MICs and MBCs of 32 mg/L. Addition of up to 4% albumin to the test medium did not change MICs and MBCs. Time-kill studies of the reference strains showed reduction rates from 3 log10 colony forming units (CFU)/ml to more than 5 log10 CFU/ml for 200 and 400 mg/L polyhexanide within 5-30 min. Testing of polyhexanide in combination with antibiotics showed indifference with amoxicillin, cefotaxime, imipenem, gentamicin, and ciprofloxacin; no antagonism was found. As no resistance and no antagonism with antibiotics were detected, polyhexanide is regarded as suitable agent for topical eradication of gram-negative bacteria.

  5. Fluorescence assay for evaluating microbicidal activity of hand antiseptics.

    Science.gov (United States)

    Lopez-Gigosos, Rosa M; Mariscal, Alberto; Mariscal-Lopez, Eloisa; Gutierrez-Bedmar, Mario; Fernandez, Joaquin

    2015-11-01

    We developed a fluorescent β-d-glucuronidase activity (BGA)-based assay for detecting and quantifying Escherichia coli in samples to assess the biocide efficacy of hand antiseptics. The fluorescence level is proportional to the number of viable E. coli organisms present. We compared our assay results to those of the E. coli plate count method specified by the European standard for testing hygienic hand rub disinfectant products (EN1500). The plate count method requires excessive handling and materials and is not valid if the number of organisms per plate is too low or high for counting in many of the samples. We optimized the fluorescent assay based on the cleavage of 4-methylumbelliferyl-β-d-glucuronide by adding 4-nitrophenyl-β-d-glucuronide, a nonfluorogenic BGA substrate, to induce glucuronidase activity and reduce assay time. Furthermore, our method can be automated and eliminates the need for multiple dilutions. Fluorescence was temporally monitored, and the time required to reach a specific value of fluorescence was correlated with the initial number of viable E. coli organisms on the samples. There was a positive correlation (P counts by plate count and fluorescence methods. Reported effects in fluorescent BGA were compared to the EN1500 plate count method with five hand disinfectants. We found our method more advantageous, because it was as sensitive as the EN1500 method, requires less time to complete, and is less expensive and less laborious than conventional plating techniques.

  6. Evaluation of antiseptic use in plastic and hand surgery.

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    Keser, Abdulmuttalip; Bozkurt, Murat; Taner, Omer Faruk; Yorgancigil, Birdal; Dogan, Metin; Sensoz, Omer

    2005-11-01

    Despite the growing complexity of plastic surgical cases, antiseptics available for preoperative preparation do not always prove effective. In addition to the varied sites of plastic surgery, there is a likelihood of considerable contamination with microorganisms since surgical gloves are frequently punctured during surgical procedures. With an aim to find a solution to all of these problems, a study was conducted in 2 stages. First, antimicrobial effects on the normal flora of hands of 4 different surgical handwashing procedures, which consist of scrubbing with 7.5% povidone-iodine (PI) scrub by using the sponge/brush, 7.5% PI scrub alone, 0.5% benzalkonium chloride (BC) solution, and 0.1% BC solution, were compared. In the second stage, bacteriological samplings were obtained from fingertips of the operating team during the surgical procedure. At the end of the study, the following results were obtained: (1) scrubbing with 7.5% PI detergent by using a scrub sponge was significantly more effective than without using a scrub sponge (P 0.05) were compared. (3) At the end of the surgery lasting 1-5 hours, hands were found to be cleaner than previously washed hands for preoperative preparation, which was considered a consequence of the sustained effect of PI. (4) There was a statistically significant difference between the cultures taken from the gloves at the end of the operation and from the hands after the gloves were taken off (P hands inside them. As a conclusion, bacterial escape due to glove perforation occurs from outside of the glove to the inside, contrary to the generally accepted concept.

  7. Synergistic effects in the short-term preservation of hides with antiseptics and gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Du Plessis, T.A.; Russell, A.E.; Stevens, R.C.B.; Galloway, A.C. (Iso-Ster (Pty) Ltd., Kempton Park (South Africa))

    1983-01-01

    The normal time lapse between the skinning and tanning processes of green hides necessitates the need for a short-term preservation technique to be employed. The most common method of bringing about such preservation is the coarse salting of the flesh side of hides. More recently the antiseptic treatment of hides was introduced to overcome the serious environmental pollution brought about by the salting process. The antiseptic treatment, however, must also be carefully controlled to avoid upsetting the biological breakdown processes in effluent plants. The gamma sterilization of such hides presents a non-polluting alternative to these methods. As the nature of this product demands excessively high radiation doses to be effective, which negatively influences the economics of the process and the physical properties of the resultant leather, a combination process employing radiation and antiseptics was investigated. It was observed that the radiation dose could be lowered from 50 kGy to 8 kGy in combination with certain antiseptics, whilst the required antiseptic concentration could be substantially lowered in the presence of radiation. The resultant leather was of an excellent quality whilst minimizing the environmental pollution problem.

  8. Synergistic effects in the short-term preservation of hides with antiseptics and gamma radiation

    Science.gov (United States)

    Du Plessis, TA; Russell, AE; Stevens, RCB; Galloway, AC

    The normal time lapse between the skinning and tanning processes of green hides necessitates the need for a short-term preservation technique to be employed. The most common method of bringing about such preservation is the coarse salting of the flesh side of hides. More recently the antiseptic treatment of hides was introduced to overcome the serious environmental pollution brought about by the salting process. The antiseptic treatment, however, must also be carefully controlled to avoid upsetting the biological breakdown processes in effluent plants. The gamma sterilization of such hides presents a non-polluting alternative to these methods. As the nature of this product demands excessively high radiation doses to be effective, which negatively influences the economics of the process and the physical properties of the resultant leather, a combination process employing radiation and antiseptics was investigated. It was observed that the radiation dose could be lowered from 50 kGy to 8 kGy in combination with certain antiseptics, whilst the required antiseptic concentration could be substantially lowered in the presence of radiation. The resultant leather was of an excellent quality whilst minimizing the environmental pollution problem.

  9. Synergistic effects in the short-term preservation of hides with antiseptics and gamma radiation

    International Nuclear Information System (INIS)

    The normal time lapse between the skinning and tanning processes of green hides necessitates the need for a short-term preservation technique to be employed. The most common method of bringing about such preservation is the coarse salting of the flesh side of hides. More recently the antiseptic treatment of hides was introduced to overcome the serious environmental pollution brought about by the salting process. The antiseptic treatment, however, must also be carefully controlled to avoid upsetting the biological breakdown processes in effluent plants. The gamma sterilization of such hides presents a non-polluting alternative to these methods. As the nature of this product demands excessively high radiation doses to be effective, which negatively influences the economics of the process and the physical properties of the resultant leather, a combination process employing radiation and antiseptics was investigated. It was observed that the radiation dose could be lowered from 50 kGy to 8 kGy in combination with certain antiseptics, whilst the required antiseptic concentration could be substantially lowered in the presence of radiation. The resultant leather was of an excellent quality whilst minimizing the environmental pollution problem. (author)

  10. Analyses comparing the antimicrobial activity and safety of current antiseptic agents: a review.

    Science.gov (United States)

    Hibbard, John S

    2005-01-01

    This article reviews the results and conclusions from four pivotal and two comparative clinical trials. The six randomized, controlled, single-blinded, parallel-group clinical trials were conducted to determine which antiseptic is best for use as a patient preoperative skin preparation. The objective of these studies was to compare the immediate, persistent (residual), and cumulative antimicrobial efficacy and safety of 2% chlorhexidine gluconate (CHG) combined with 70% isopropyl alcohol (IPA) (ChloraPrep); another combination CHG and IPA antiseptic (CHG+IPA) and 2% aqueous CHG alone; 4% CHG (Hibiclens) alone; 70% isopropyl alcohol (IPA) alone; and an iodine-containing solution, 10% povidone-iodine (Betadine) alone as preoperative skin topical antiseptics for potential prevention of nosocomial infections.

  11. Identification and compatibility of the major active principles in some new natural origin antiseptics

    Science.gov (United States)

    Nikolić, G. S.; Zlatković, S.; Nikolić, N.

    2009-09-01

    The newly established instrumentation of HPLC/DAD, FTIR, and NMR techniques have been applied for simultaneous identification and physicochemical compatibility determination of the potential major antiseptic constituents ( Hypericum perforatum L. and Usnea barbata extracts) which can be present in some new origin pharmaceutical preparation. Based on the obtained results the conclusion is that a simultaneous use of the analyzed constituents in production of some new preparations with antiseptic properties is possible. The chromatographic separation of antiseptic mixture was performed on a RP-HPLC C18 column. For the NMR detection, the analytes eluted from LC column were trapped and hereafter transported into the NMR flow-cell. The NMR and FTIR techniques allowed the characterization of the major constituent of Hypericum perforatum L., mainly hypericin, as well as of Usnea barbata, mainly usnic acid.

  12. Antiseptics and disinfectants for the treatment of bacterial vaginosis: A systematic review

    Directory of Open Access Journals (Sweden)

    Verstraelen Hans

    2012-06-01

    Full Text Available Abstract Background The study objective was to assess the available data on efficacy and tolerability of antiseptics and disinfectants in treating bacterial vaginosis (BV. Methods A systematic search was conducted by consulting PubMed (1966-2010, CINAHL (1982-2010, IPA (1970-2010, and the Cochrane CENTRAL databases. Clinical trials were searched for by the generic names of all antiseptics and disinfectants listed in the Anatomical Therapeutic Chemical (ATC Classification System under the code D08A. Clinical trials were considered eligible if the efficacy of antiseptics and disinfectants in the treatment of BV was assessed in comparison to placebo or standard antibiotic treatment with metronidazole or clindamycin and if diagnosis of BV relied on standard criteria such as Amsel’s and Nugent’s criteria. Results A total of 262 articles were found, of which 15 reports on clinical trials were assessed. Of these, four randomised controlled trials (RCTs were withheld from analysis. Reasons for exclusion were primarily the lack of standard criteria to diagnose BV or to assess cure, and control treatment not involving placebo or standard antibiotic treatment. Risk of bias for the included studies was assessed with the Cochrane Collaboration’s tool for assessing risk of bias. Three studies showed non-inferiority of chlorhexidine and polyhexamethylene biguanide compared to metronidazole or clindamycin. One RCT found that a single vaginal douche with hydrogen peroxide was slightly, though significantly less effective than a single oral dose of metronidazole. Conclusion The use of antiseptics and disinfectants for the treatment of BV has been poorly studied and most studies are somehow methodologically flawed. There is insufficient evidence at present to advocate the use of these agents, although some studies suggest that some antiseptics may have equal efficacy compared to clindamycin or metronidazole. Further study is warranted with special regard to

  13. Efficacy of a novel skin antiseptic against carbapenem-resistant Enterobacteriaceae.

    Science.gov (United States)

    Wiemken, Timothy L; Kelley, Robert R; Carrico, Ruth M; Binford, Laura E; Guinn, Brian E; Mattingly, William A; Peyrani, Paula; Ramirez, Julio A

    2015-04-01

    Infections caused by carbapenem-resistant Enterobacteriaceae (CRE) are increasing on a global scale. Because of the need for CRE transmission prevention and control, we sought to evaluate the efficacy of a silver-based skin antiseptic against these organisms. Using a human skin analog, a third party laboratory conducted efficacy testing. The results suggest that this product provides antimicrobial activity against CRE on human skin. Because of the unique properties, this antiseptic may be useful for daily bathing of hospitalized patients to assist in the control of CRE.

  14. [THE ROLE OF ANTISEPTICS AND STRATEGY OF BIOFILM REMOVAL IN CHRONIC WOUND].

    Science.gov (United States)

    Kucisec-Tepes, Nastja

    2016-03-01

    Chronic wound does not heal within the expected time frame because it remains in the inflammation phase of healing. The reason for this is the presence of necrotic tissue and a large number of microorganisms, primarily bacteria that secrete the biofilm, along with ischemia, hypoxia and edema. Biofilm is present in 90% of chronic wounds and 6% of the acute ones. Biofilm is a corporative association of microbes which adhere to the surface of the wound, guided by quorum sensing molecules. The association is surrounded by a moisturizing matrix of extracellular polymeric substances (slime) which protect the microbes from the impact of antibiotics, antiseptics, macro-organism defense and stress. Biofilm is the primary cause of the wound chronicity because it causes permanent inflammation, delayed granulation tissue formation and migration of epithelium cells, thus providing a reservoir of microbes that lead to infection of the chronic wound. The aim of good clinical practice is to enable healing of a chronic wound within the expected time frame. In order to achieve this aim, it is necessary to reduce and thoroughly remove the biofilm from the wound and prevent its reappearance. This is achieved by the application of active anti-biofilm compounds and procedures that disintegrate the quorum sensing molecules, degrade the extracellular polymeric substances and block adherence to the surfaces. Recent researches have shown that the application of antiseptics is effective in the prevention of infection and is a support to targeted treatment. However, the fact is that only some antiseptics are applicable to chronic wounds and can have an impact on biofilms of the primary infective agents such as Staphylococcus spp., Streptococcus spp., and Pseudomonas aeruginosa. Effective antiseptics are octenidine dihydrochloride, polyhexanides, povidone and cadexomer iodine, nanocrystal silver and Manuka-type honey. Immobile biofilm is a persistent problem of chronic and chronic infected

  15. [Analysis of antibacterial activity of a new antiseptic for therapy of maxillofacial inflammations].

    Science.gov (United States)

    Leont'ev, V K; Kuznetsova, L I; Leshchankina, E L; Kuznetsov, V L

    2002-01-01

    Clinical and experimental studies of polydimethyldiallylammonium chloride (PDMDAAC) experimentally validated the choice of optimal concentration and composition of this agent. The results indicate a high antiseptic activity of 1% PDMDAAC and the possibility of using it in local therapy of periodontitis and posttraumatic infection.

  16. 77 FR 71804 - Antiseptic Patient Preoperative Skin Preparation Products; Public Hearing; Request for Comments...

    Science.gov (United States)

    2012-12-04

    ... (FDA) is correcting a document that appeared in the Federal Register of November 21, 2012 (77 FR 69863... INFORMATION: Correction In the Federal Register of November 21, 2012, in FR Doc. 2012- 28357, on page 69863... HUMAN SERVICES Food and Drug Administration Antiseptic Patient Preoperative Skin Preparation...

  17. Use of antiseptics in preparing feed yeast for the production of alcohol from cereals and potatoes

    Energy Technology Data Exchange (ETDEWEB)

    Cojocaru, C.; Iliescu, V.

    1959-01-01

    When the breeding vessels are made antiseptic by NH/sub 4/F, fermentation starts immediately upon seeding. The fermentation cycle is shortened by 16%. No abnormal increase in acidity is observed. The alcohol yield is increased by 1.8%.

  18. 食品防腐剂的研究现状%Research Progress of Food Antiseptic

    Institute of Scientific and Technical Information of China (English)

    惠秋沙

    2011-01-01

    The application and research status of food antiseptic in recent years were reviewed and its development trend wa described.%综述了最近几年食品防腐剂的应用和研究现状,阐述了食品防腐剂的发展趋势。

  19. Efficacy of two ethanol-based skin antiseptics on the forehead at shorter application times

    Directory of Open Access Journals (Sweden)

    Pitten Frank-Albert

    2007-09-01

    Full Text Available Abstract Background Recent research suggests that alcohol-based skin antiseptics exhibit their efficacy on the resident skin flora of the forehead in less than 10 minutes. That is why we have looked at the efficacy of two ethanol-based skin antiseptics applied for 10, 2.5 and 2 minutes on skin with a high density of sebaceous glands. Each experiment was performed in a reference-controlled cross-over design with at least 20 participants. Application of isopropanol (70%, v/v for 10 minutes to the forehead served as the reference treatment. The clear (skin antiseptic A and coloured preparations (skin antiseptic B contain 85% ethanol (w/w. Pre-values and post-values (immediately after the application and after 30 min were obtained by swabbing a marked area of 5 cm2 for about 10 s. Swabs were vortexed in tryptic soy broth containing valid neutralizing agents. After serial dilution aliquots were spread on tryptic soy agar. Colonies were counted after incubation of plates at 36°C for 48 h. The mean log10 reduction of bacteria was calculated. The Wilcoxon matched-pairs signed-ranks test was used for a comparison of treatments. Results Skin antiseptic A applied for 10 min was significantly more effective than the reference treatment. When applied for 2.5 min (three experiments it was significantly more effective than the reference treatment immediately after application (2.7 versus 2.2 log10 reduction; p 10 reduction; p = 0.053. Skin antiseptic B applied for 2.5 min (three experiments was significantly more effective than the reference treatment both immediately after application (2.3 versus 1.9 log10 reduction; p 10 reduction; p = 0.002. Conclusion The clear and coloured skin antiseptics applied for 2.5 min on the skin of the forehead fulfilled the efficacy requirements for skin antisepsis. The shorter application time on skin with a high density of sebaceous glands will allow to act more efficiently in clinical practice.

  20. The effect of two different oral antiseptics on dental plaque formation (De Novo Biofilm) and on gingival inflammation

    OpenAIRE

    Zelić Obrad; Čakić Saša; Luković Natalija

    2009-01-01

    Introduction. Since the discovery that periodontal diseases are caused by microbial plaque the interest of many scientists has been focused on oral antiseptics. There are very few mouthrinses with oral antiseptic effect originally designed in our country. One of these is Ozosept® solution. Objective. This study evaluated the effect of Ozosept® solution (phenol compound) on the oral hygiene and gingival inflammation, in comparison with Hibidex DAP® solution (chlorhexidine digluconate). Methods...

  1. ANTISEPTIC SELECTION RATIONALE FOR COMPLEX NASAL MEDICINE HAVING ANTIMI- CROBIAL AND VASOCONSTRICTOR ACTION

    Directory of Open Access Journals (Sweden)

    Boyko N.N.

    2013-12-01

    Full Text Available Screening of antimicrobial properties of Myramistinum 0.01 % solution, Decasanum 0.02 % solution, Chlorhexdine digluconate 0.05 % solution, Benzalkonium chloride 0.05 % solution, and Octenisept 0.1 % solution has been carried out. It has been found that Chlorhexidine digluconate 0.05 % solution has the greatest antimicrobial activity, whereas Myramistinum 0.01 % solution has the lowest activity. For manifestation of optimal antiseptic properties of cation-active antiseptics, it is proposed to increase their concentration in the preparation by not less than 0.05% and not more than 0.1%. It is noted that use of Octenisept is inefficient due to its expensiveness and high concentration needed to achieve the optimal level ofmanifestation of its anti-microbial properties.

  2. In vitro investigation of the follicular penetration of porcine ear skin using a nanoparticle-emulsion containing the antiseptic polihexanide

    International Nuclear Information System (INIS)

    Earlier investigations regarding the distribution of the bacterial flora on the human skin demonstrate that the hair follicle acts as a bacterial reservoir, providing a quick source for secondary recontamination. These findings highlight the importance of the hair follicle as a target for modern antiseptics. In the present study, we have assessed the follicular penetration of a curcumin-labeled particle-associated antiseptic into porcine skin by laser scanning microscopy. Therefore, the follicular penetration depth of the curcumin-labeled particle-associated antiseptic was compared to the follicular penetration depth of curcumin-labeled particles without antiseptic. The investigation was performed in vitro using porcine skin biopsies. By superposition of the images acquired in the transmission and the fluorescent modus, it was possible to visualize the distribution of the fluorescent dye inside the hair follicles. Quantitative and qualitative results showed that both dispersions penetrated efficiently into the hair follicles. The average penetration depth of the particles with attached antiseptic polihexanide was significantly higher than that of particles without the attached antiseptic. Also, whilst very little sample preparation was needed, laser scanning microscopy was found to be an efficient tool to visualize the skin relief and in particular the hair follicle shaft and localize fluorescent markers within the skin tissue and hair follicles

  3. Evaluation of a routine antiseptic and two disinfectants for reducing bacterial population of cow hoof

    OpenAIRE

    Moosa Javdani,; Seifollah Dehghani,; Ali Ghashghaii; Zahra Nikousefat

    2011-01-01

    A routine antiseptic and two disinfectant agents were used separately for reducing bacterial population of cow hoof: 1) 7.5% povidone–iodine scrub mixed with 10% povidone–iodine solution, 2) 10% copper sulfate, and 3) 8% formaldehyde. Swabbing for microbial colony counts were used to evaluate pre and post–scrub of hooves of eight cows. The results revealed no significant differences in reduction of bacterial colony count between post–scrubs of povidone–iodine and formaldehyde. B...

  4. Ex vivo vs. in vivo antibacterial activity of two antiseptics on oral biofilm

    Directory of Open Access Journals (Sweden)

    ISABEL ePRADA-LÓPEZ

    2015-07-01

    Full Text Available Aim: To compare the immediate antibacterial effect of two application methods (passive immersion and active mouthwash of two antiseptic solutions on the in situ oral biofilm.Material and Methods: A randomized observer-masked crossover study was conducted. Fifteen healthy volunteers wore a specific intraoral device for 48 hours to form a biofilm in three glass disks. One of these disks was used as a baseline; another one was immersed in a solution of 0.2% Chlorhexidine (0.2% CHX, remaining the third in the device, placed in the oral cavity, during the 0.2% CHX mouthwash application. After a two-week washout period, the protocol was repeated using a solution of Essential Oils (EO. Samples were analysed for bacterial viability with the confocal laser scanning microscope after previous staining with LIVE/DEAD® BacLight™.Results: The EO showed a better antibacterial effect compared to the 0.2% CHX after the mouthwash application (% of bacterial viability= 1.16 ± 1.00% vs. 5.08 ± 5.79%, respectively, and was more effective in all layers (p<0.05. In the immersion, both antiseptics were significantly less effective (% of bacterial viability= 26.93 ± 13.11%, EO vs. 15.17 ± 6.14%, 0.2% CHX; in the case of EO immersion, there were no significant changes in the bacterial viability of the deepest layer in comparison with the baseline. Conclusions: The method of application conditioned the antibacterial activity of the 0.2% CHX and EO solutions on the in situ oral biofilm. The in vivo active mouthwash was more effective than the ex vivo passive immersion in both antiseptic solutions. There was more penetration of the antiseptic inside the biofilm with an active mouthwash, especially with the EO.

  5. Partitioning, Persistence, and Accumulation in Digested Sludge of the Topical Antiseptic Triclocarban During Wastewater Treatment

    OpenAIRE

    Heidler, Jochen; Sapkota, Amir; Halden, Rolf U.

    2006-01-01

    The topical antiseptic agent triclocarban (TCC) is a common additive in many antimicrobial household consumables, including soaps and other personal care products. Long-term usage of the mass-produced compound and a lack of understanding of its fate during sewage treatment motivated the present mass balance analysis conducted at a typical U.S. activated sludge wastewater treatment plant featuring a design capacity of 680 million liters per day. Using automated samplers and grab sampling, the ...

  6. In vitro antimicrobial activity of a commercial ear antiseptic containing chlorhexidine and Tris-EDTA.

    Science.gov (United States)

    Guardabassi, Luca; Ghibaudo, Giovanni; Damborg, Peter

    2010-06-01

    Minimum bactericidal concentrations (MBCs) of a commercial ear antiseptic containing chlorhexidine 0.15% and Tris-EDTA (Otodine) were determined by broth microdilution for 150 isolates representing the most common pathogens associated with canine otitis. The microorganisms were classified into three groups according to their levels of susceptibility. The most susceptible group included Staphylococcus pseudintermedius, Malassezia pachydermatis, Streptococcus canis and Corynebacterium auriscanis, which were generally killed by 1 : 64 dilution of the antiseptic product (MBC = 23/0.8 microg/mL of chlorhexidine/Tris-EDTA). The most resistant organism was Proteus mirabilis, which survived up to 1 : 8 dilution of the product (MBC = 375/12 microg/mL). Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus displayed intermediate MBCs ranging between 188/6 and 47/1.5 microg/mL. Interestingly, S. pseudintermedius was more susceptible than S. aureus, and no significant difference was observed between meticillin-resistant and meticillin-susceptible isolates within each species, indicating that antiseptic use is unlikely to co-select for meticillin resistance. Although the concentrations required for killing (MBCs) varied considerably with microorganism type, the combination of chlorhexidine 0.15% and Tris-EDTA was active against all the pathogens most commonly involved in canine otitis. PMID:20030799

  7. Povidone-iodine: use in hand disinfection, skin preparation and antiseptic irrigation.

    Science.gov (United States)

    Durani, Piyush; Leaper, David

    2008-06-01

    Iodine and its antibacterial properties have been used for the prevention or management of wound infections for over 150 years. However, the use of solutions (tincture) of iodine has been replaced by the widespread use of povidone-iodine, a water-soluble compound, which is a combination of molecular iodine and polyvinylpyrrolidone. The resultant broad spectrum of antimicrobial activity is well documented and its efficacy, particularly in relation to resistant micro-organisms such as methicillin-resistant Staphylococcus aureus, has been shown. In the clinical environment, there is no general agreement regarding the 'best' antiseptic and the practice varies widely. This article reviews the studies that have assessed the efficacy of povidone-iodine in hand disinfection and skin preparation and its use as an antiseptic irrigant. Although there is a distinct lack of well-designed, randomised controlled trials evaluating antiseptic efficacy, selection should be based on the next best available evidence. This evidence suggests that the use of povidone-iodine as an agent of choice is dependent on the clinical need but is also likely to be influenced by personal preference.

  8. Antiseptic properties of two calix[4]arenes derivatives on the human coronavirus 229E.

    Science.gov (United States)

    Geller, C; Fontanay, S; Mourer, M; Dibama, H Massimba; Regnouf-de-Vains, J-B; Finance, C; Duval, R E

    2010-12-01

    Facing the lack in specific antiviral treatment, it is necessary to develop new means of prevention. In the case of the Coronaviridae this family is now recognized as including potent human pathogens causing upper and lower respiratory tract infections as well as nosocomial ones. Within the purpose of developing new antiseptics molecules, the antiseptic virucidal activity of two calix[4]arene derivatives, the tetra-para-sulfonato-calix[4]arene (C[4]S) and the 1,3-bis(bithiazolyl)-tetra-para-sulfonato-calix[4]arene (C[4]S-BTZ) were evaluated toward the human coronavirus 229E (HCoV 229E). Comparing these results with some obtained previously with chlorhexidine and hexamidine, (i) these two calixarenes did not show any cytotoxicity contrary to chlorhexidine and hexamidine, (ii) C[4]S showed as did hexamidine, a very weak activity against HCoV 229E, and (iii) the C[4]S-BTZ showed a stronger activity than chlorhexidine, i.e. 2.7 and 1.4log₁₀ reduction in viral titer after 5min of contact with 10⁻³mol L⁻¹ solutions of C[4]S-BTZ and chlorhexidine, respectively. Thus, the C[4]S-BTZ appeared as a promising virucidal (antiseptic) molecule.

  9. In vitro antimicrobial activity of a commercial ear antiseptic containing chlorhexidine and Tris-EDTA.

    Science.gov (United States)

    Guardabassi, Luca; Ghibaudo, Giovanni; Damborg, Peter

    2010-06-01

    Minimum bactericidal concentrations (MBCs) of a commercial ear antiseptic containing chlorhexidine 0.15% and Tris-EDTA (Otodine) were determined by broth microdilution for 150 isolates representing the most common pathogens associated with canine otitis. The microorganisms were classified into three groups according to their levels of susceptibility. The most susceptible group included Staphylococcus pseudintermedius, Malassezia pachydermatis, Streptococcus canis and Corynebacterium auriscanis, which were generally killed by 1 : 64 dilution of the antiseptic product (MBC = 23/0.8 microg/mL of chlorhexidine/Tris-EDTA). The most resistant organism was Proteus mirabilis, which survived up to 1 : 8 dilution of the product (MBC = 375/12 microg/mL). Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus displayed intermediate MBCs ranging between 188/6 and 47/1.5 microg/mL. Interestingly, S. pseudintermedius was more susceptible than S. aureus, and no significant difference was observed between meticillin-resistant and meticillin-susceptible isolates within each species, indicating that antiseptic use is unlikely to co-select for meticillin resistance. Although the concentrations required for killing (MBCs) varied considerably with microorganism type, the combination of chlorhexidine 0.15% and Tris-EDTA was active against all the pathogens most commonly involved in canine otitis.

  10. Choline and Geranate Deep Eutectic Solvent as a Broad-Spectrum Antiseptic Agent for Preventive and Therapeutic Applications.

    Science.gov (United States)

    Zakrewsky, Michael; Banerjee, Amrita; Apte, Sanjana; Kern, Theresa L; Jones, Mattie R; Sesto, Rico E Del; Koppisch, Andrew T; Fox, David T; Mitragotri, Samir

    2016-06-01

    Antiseptic agents are the primary arsenal to disinfect skin and prevent pathogens spreading within the host as well as into the surroundings; however the Food and Drug Administration published a report in 2015 requiring additional validation of nearly all current antiseptic agents before their continued use can be allowed. This vulnerable position calls for urgent identification of novel antiseptic agents. Recently, the ability of a deep eutectic, Choline And Geranate (CAGE), to treat biofilms of Pseudomonas aeruginosa and Salmonella enterica was demonstrated. Here it is reported that CAGE exhibits broad-spectrum antimicrobial activity against a number of drug-resistant bacteria, fungi, and viruses including clinical isolates of Mycobacterium tuberculosis, Staphylococcus aureus, and Candida albicans as well as laboratory strains of Herpes Simplex Virus. Studies in human keratinocytes and mice show that CAGE affords negligible local or systemic toxicity, and an ≈180-14 000-fold improved efficacy/toxicity ratio over currently used antiseptic agents. Further, CAGE penetrates deep into the dermis and treats pathogens located in deep skin layers as confirmed by the ability of CAGE in vivo to treat Propionibacterium acnes infection. In combination, the results clearly demonstrate CAGE holds promise as a transformative platform antiseptic agent for preventive as well as therapeutic applications. PMID:26959835

  11. [Comparison of the efficacy of 2 antiseptic solutions in the prevention of infection from peridural catheters].

    Science.gov (United States)

    Adam, M N; Dinulescu, T; Mathieu, P; Giacomini, T; Le Pennec, M P

    1996-01-01

    Two antiseptic solutions (iodine polyvidone and chlorhexidine) were compared-in a prospective non-randomized study including 294 parturient women. This study aimed to assess their efficacy against infections through epidural catheters. All catheters were subsequently cultivated. Cultures were significantly positive in 3% of cases after iodine polyvidone and 1% after chlorhexidine decontamination (not significant). No clinical or biological infections were detected. Notwithstanding some apparently unavoidable but moderate contaminations, prevention of infections post epidural analgesia depends principally on a complete adherence to asepsia rules.

  12. Characterization of antiseptic apatite powders prepared at biomimetics temperature and pH

    Directory of Open Access Journals (Sweden)

    Soumia Belouafa

    2008-03-01

    Full Text Available Antiseptic apatite-based calcium phosphates were prepared as the single-phase powders. Phosphocalcic oxygenated apatites were synthesized from calcium salts and orthophosphate dissolved in oxygenated water solution at 30%, under the biomimetic conditions of 37 °C and pH 7.4. The characterization and chemical analysis of the synthesized biomimetic apatite powders were performed by scanning electron microscopy (SEM, powder X ray diffraction (XRD, Fourier-transformed infrared spectroscopy (FT-IR and chemical analysis. The obtained materials are a calcium deficient apatites with different morphologies.

  13. 2,3,4,6-Tetrachlorophenyl esters as antiseptics for fibrous materials

    Energy Technology Data Exchange (ETDEWEB)

    Stepanenko, N.I.; Ryakhovskaya, A.I.; Romanenko, O.A.; Garifullina, K.R.

    1983-09-10

    Wastes containing tri- and tetrachlorobenzenes are obtained during production of di- and trichlorobenzenes. One way of utilizing these wastes is conversion into di- and trichlorophenols by saponification. Chlorination of a mixture of di- and trichlorophenols converts it into 2,3,4,6-tetrachlorophenol with high yields (95-96 wt. %). The uses of tetrachlorophenol are limited by its strong specific odor and volatility. The possibility of obtaining esters of 2,3,4,6-tetrachlorophenol and saturated aliphatic C/sub 2/-C/sub 12/ monocarboxylic acids, which have good antiseptic properties in relation to fibrous materials are described.

  14. Treating infected diabetic wounds with superoxidized water as anti-septic agent: a preliminary experience

    International Nuclear Information System (INIS)

    To evaluate the effectiveness of superoxidized water (MicrocynTM) in diabetic patients with different wounds. One hundred known diabetic patients were enrolled. Half were randomized to the intervention group (those whose wounds were managed with superoxidized water) and half to the control group (whose wounds were treated with normal saline) using a table of random numbers. The two groups were matched for age, gender, duration of diabetes and category of wound. All patients received appropriate surgical treatment for their wounds as required. Local wound treatment was carried out daily using superoxidized water soaked gauzes on twice daily basis in the intervention group and normal saline in the control group. The treatment was continued until wound healing. The main outcome measures were duration of hospital stay, downgrading of the wound category, wound healing time and need for interventions such as amputation. Statistically significant differences were found in favour of the superoxidized water group with respect to duration of hospital stay, downgrading of the wound category and wound healing time. Although the initial results of employing superoxidized water for the management of infected diabetic wounds are encouraging, further multicentre clinical trials are warranted before this antiseptic is recommended for general use. It may offer an economical alternative to other expensive antiseptics with positive impact on the prevailing infection rates, patient outcomes and patient satisfaction. (author)

  15. Inhibition of the anti-staphylococcal activity of the antiseptic polihexanide by mucin.

    Science.gov (United States)

    Ansorg, Rainer; Rath, Peter-Michael; Fabry, Werner

    2003-01-01

    The antiseptic Lavasept (LS), containing the polymeric biguanide polihexanide (CAS 28757-48-4), possesses microbicidal activity against a broad spectrum of bacteria including Staphylococcus aureus. It is used for antiseptic wound care in concentrations corresponding to 0.2-0.4 mg polihexanide per ml. To obtain basic data on its ability to eradicate S. aureus colonizing the nasal mucosa, the influence of mucin on the anti-staphylococcal activity was investigated. A disk agar-diffusion method was applied. Two reference strains of S. aureus (methicillin-sensitive S. aureus ATCC 29213 and methicillin-resistant S. aureus ATCC 33591) and 20 fresh clinical isolates were used. In the absence of mucin, the growth of all strains was inhibited by polihexanide concentrations of 0.1 mg/ml. In the presence of 0.25% mucin in the test medium, a concentration of 0.4 mg/ml was necessary to inhibit all strains. Mucin concentrations of 0.5% and 1%, that are even lower than the mucin concentrations in healthy nasal secretions, abolished the activity of the therapeutic concentrations of polihexanide. It is concluded that the inactivation of LS by mucin obstructs a reliable clearance of nasal S. aureus carriage.

  16. Controlled antiseptic/eosin release from chitosan-based hydrogel modified fibrous substrates.

    Science.gov (United States)

    Romano, Ilaria; Ayadi, Farouk; Rizzello, Loris; Summa, Maria; Bertorelli, Rosalia; Pompa, Pier Paolo; Brandi, Fernando; Bayer, Ilker S; Athanassiou, Athanassia

    2015-10-20

    Fibers of cellulose networks were stably coated with N-methacrylate glycol chitosan (MGC) shells using subsequent steps of dip coating and photo-curing. The photo-crosslinked MGC-coated cellulose networks preserved their fibrous structure. A model hydrophilic antiseptic solution containing eosin, chloroxylenol and propylene glycol was incorporated into the shells to study the drug release dynamics. Detailed drug release mechanism into phosphate buffered saline (PBS) solutions from coated and pristine fibers loaded with the antiseptic was investigated. The results show that the MGC-coated cellulose fibers enable the controlled gradual release of the drug for four days, as opposed to fast, instantaneous release from eosin coated pristine fibers. This release behavior was found to affect the antibacterial efficiency of the fibrous cellulose sheets significantly against Staphylococcus aureus and Candida albicans. In the case of the MGC-eosin functionalized system the antibacterial efficiency was as high as 85% and 90%, respectively, while for the eosin coated pristine cellulose system the efficiency was negative, indicating bacterial proliferation. Furthermore, the MGC-eosin system was shown to be efficacious in a model of wound healing in mice, reducing the levels of various pro-inflammatory cytokines that modulate early inflammatory phase responses. The results demonstrate good potential of these coated fibers for wound dressing and healing applications. Due to its easy application on common passive commercial fibrous dressings such as gauzes and cotton fibers, the method can render them active dressings in a cost effective way. PMID:26256189

  17. Antiseptic wick: does it reduce the incidence of wound infection following appendectomy?

    LENUS (Irish Health Repository)

    McGreal, Gerald T

    2012-02-03

    The role of prophylactic antibiotics is well established for contaminated wounds, but the use of antiseptic wound wicks is controversial. The aim of this work was to study the potential use of wound wicks to reduce the rate of infection following appendectomy. This prospective randomized controlled clinical trial was conducted at a university hospital in the department of surgery. The subjects were patients undergoing appendectomy for definite acute appendicitis. They were randomized by computer to primary subcuticular wound closure or use of an antiseptic wound wick. For the latter, ribbon gauze soaked in povidone-iodine was placed between interrupted nylon skin sutures. Wicks were soaked daily and removed on the fourth postoperative day. All patients received antibiotic prophylaxis. They were reviewed while in hospital and 4 weeks following operation for evidence of wound infection. The main outcome measures were wound infection, wound discomfort, and cosmetic result. The overall wound infection rate was 8.6% (15\\/174). In patients with wound wicks it was 11.6% (10\\/86) compared to 5.6% (5\\/88) in those whose wounds were closed by subcuticular sutures (p = NS). We concluded that the use of wound wicks was not associated with decreased wound infection rates following appendectomy. Subcuticular closure is therefore appropriate in view of its greater convenience and safety.

  18. Controlled antiseptic/eosin release from chitosan-based hydrogel modified fibrous substrates.

    Science.gov (United States)

    Romano, Ilaria; Ayadi, Farouk; Rizzello, Loris; Summa, Maria; Bertorelli, Rosalia; Pompa, Pier Paolo; Brandi, Fernando; Bayer, Ilker S; Athanassiou, Athanassia

    2015-10-20

    Fibers of cellulose networks were stably coated with N-methacrylate glycol chitosan (MGC) shells using subsequent steps of dip coating and photo-curing. The photo-crosslinked MGC-coated cellulose networks preserved their fibrous structure. A model hydrophilic antiseptic solution containing eosin, chloroxylenol and propylene glycol was incorporated into the shells to study the drug release dynamics. Detailed drug release mechanism into phosphate buffered saline (PBS) solutions from coated and pristine fibers loaded with the antiseptic was investigated. The results show that the MGC-coated cellulose fibers enable the controlled gradual release of the drug for four days, as opposed to fast, instantaneous release from eosin coated pristine fibers. This release behavior was found to affect the antibacterial efficiency of the fibrous cellulose sheets significantly against Staphylococcus aureus and Candida albicans. In the case of the MGC-eosin functionalized system the antibacterial efficiency was as high as 85% and 90%, respectively, while for the eosin coated pristine cellulose system the efficiency was negative, indicating bacterial proliferation. Furthermore, the MGC-eosin system was shown to be efficacious in a model of wound healing in mice, reducing the levels of various pro-inflammatory cytokines that modulate early inflammatory phase responses. The results demonstrate good potential of these coated fibers for wound dressing and healing applications. Due to its easy application on common passive commercial fibrous dressings such as gauzes and cotton fibers, the method can render them active dressings in a cost effective way.

  19. Formaldehyde as an antiseptic in the production of alcohol from molasses

    Energy Technology Data Exchange (ETDEWEB)

    Rosa, M.; Vernerova, J.; Stros, F.

    1962-01-01

    The effect of formaldehyde as an antiseptic for the noncontaminated alcohol fermentation of molasses was examined with five different yeast-mash batches containing formaldehyde 0.01%. Following 24 hour cultivation, increase in the activity of formaldehyde-inoculated mash was lowered by 47%; the formaldehyde decreased the alcohol fermentation and lessened the rate of fermentation of sugar by 20% in noncontaminated mash. The decrease in the fermentation rate was also observed during the entire course of fermentation. On the other hand, formaldehyde addition to molasses contaminated with lactic acid bacteria accelerated the fermentation rate by inhibiting the bacterial growth; the degree of retardation due to the contamination was greater than that due to the antiseptic. The view that formaldehyde decreased the effectiveness of growth substances by binding the amino acid present in mash mixtures could not be confirmed; it was believed that formaldehyde was absorbed by yeast cells even through the entities thus destroyed could not be differentiated from living cells by methylene blue. Higher resistance of yeast cultures to formaldehyde was obtained by repeated transfers of the yeast in media containing formaldehyde. It was concluded that formaldehyde added to contaminated molasses inhibited the further growth of the contamination, increased yield of ethanol on the basis of the sugar, and simultaneously decreased the net yield of ethanol in contrast to the yield from noncontaminated mashes in the absence of formaldehyde.

  20. Irritancy of antiseptics tested by repeated open exposures on the human skin, evaluated by non-invasive methods

    NARCIS (Netherlands)

    Tupker, RA; Schuur, J; Coenraads, PJ

    1997-01-01

    The aim of the study was to test the irritancy of 6 antiseptics in an open exposure model. The following agents were tested in their normal use concentrations using open exposures, 2x daily for 4 days in 20 subjects: chlorhexidine 4% (CH), chlorhexidine 0.5% in ethanol 70% (CE), ethanol 70% (ET), io

  1. Evaluation of a routine antiseptic and two disinfectants for reducing bacterial population of cow hoof

    Directory of Open Access Journals (Sweden)

    Moosa Javdani,

    2011-03-01

    Full Text Available A routine antiseptic and two disinfectant agents were used separately for reducing bacterial population of cow hoof: 1 7.5% povidone–iodine scrub mixed with 10% povidone–iodine solution, 2 10% copper sulfate, and 3 8% formaldehyde. Swabbing for microbial colony counts were used to evaluate pre and post–scrub of hooves of eight cows. The results revealed no significant differences in reduction of bacterial colony count between post–scrubs of povidone–iodine and formaldehyde. Bacterial colony counts after the povidone–iodine scrub solution and formaldehyde scrub were significantly different from those obtained after the copper sulfate scrub. Significant reduction in number of microbial colony in post–scrub by povidone–iodine, formaldehyde, and copper sulfate were observed which were different from the control (warm tap water.

  2. In vitro evaluation of the antimicrobial activity of HM-242, a novel antiseptic compound.

    Science.gov (United States)

    Okunishi, Junji; Nishihara, Yutaka; Maeda, Shirou; Ikeda, Masahiro

    2009-09-01

    The antimicrobial activities of N(4)-octyl-6,6-dimethyl-N(2)-(4-methylbenzyl)-1,6-dihydro-1,3,5-triazine-2,4-diamine (HM-242), a novel synthetic compound, were compared with those of chlorhexidine gluconate (CHG). HM-242 was a more potent microbicide than CHG in vitro; however, its minimal inhibitory concentrations were similar. In particular, HM-242 killed various Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis, both efficiently and rapidly. HM-242 also showed potent virucidal activity against enveloped viruses such as influenza virus and herpes simplex virus. These characteristics suggest that HM-242 may well be useful as an antiseptic.

  3. Collective relaxation dynamics and crystallization kinetics of the amorphous Biclotymol antiseptic.

    Science.gov (United States)

    Tripathi, Pragya; Romanini, Michela; Tamarit, Josep Lluis; Macovez, Roberto

    2015-11-10

    We employ dielectric spectroscopy to monitor the relaxation dynamics and crystallization kinetics of the Biclotymol antiseptic in its amorphous phase. The glass transition temperature of the material as determined by dielectric spectroscopy is Tg = 290 ± 1K. The primary (α) relaxation dynamics is observed to follow a Vogel-Fulcher-Tammann temperature dependence, with a kinetic fragility index m = 86 ± 13, which classifies Biclotymol as a relatively fragile glass former. A secondary relaxation is also observed, corresponding to an intramolecular dynamic process of the non-rigid Biclotymol molecule. The crystallization kinetics, measured at four different temperatures above the glass transition temperature, follows an Avrami behavior with exponent virtually equal to n = 2, indicating one-dimensional crystallization into needle-like crystallites, as experimentally observed, with a time-constant nucleation rate. The activation barrier for crystallization is found to be Ea = 115 ± 22 kJ mol(-1). PMID:26364713

  4. Thermodynamic clarification of interaction between antiseptic compounds and lipids consisting of stratum corneum

    International Nuclear Information System (INIS)

    The interactions of antiseptic compounds with quaternary ammonium, such as benzalkonium chloride (BC), benzethonium chloride (BZC), dodecyldiaminoethyl-glycine hydrochloride (AEG), and chlorhexidine gluconate (CHG), with components of the stratum corneum were investigated by isothermal titration calorimetry at pH 7.5 and 25 deg. C. The different mechanisms for their permeation to stratum corneum were clarified. Cationic surfactants of BC and BZC bound to cholesterol and cholesterol sulfate with high affinity (105-106 M-1) to extract endogenous cholesterol and its derivatives from the stratum corneum and penetrated via an intercellular route. CHG also bound to cholesterol and accumulated in the stratum corneum without removing endogenous cholesterol. On the other hand, an amphoteric surfactant of AEG seemed to be incorporated into the lipid bilayer and bound to ceramide with its polar end close to the lipid polar heads by hydrophobic interaction

  5. Collective relaxation dynamics and crystallization kinetics of the amorphous Biclotymol antiseptic.

    Science.gov (United States)

    Tripathi, Pragya; Romanini, Michela; Tamarit, Josep Lluis; Macovez, Roberto

    2015-11-10

    We employ dielectric spectroscopy to monitor the relaxation dynamics and crystallization kinetics of the Biclotymol antiseptic in its amorphous phase. The glass transition temperature of the material as determined by dielectric spectroscopy is Tg = 290 ± 1K. The primary (α) relaxation dynamics is observed to follow a Vogel-Fulcher-Tammann temperature dependence, with a kinetic fragility index m = 86 ± 13, which classifies Biclotymol as a relatively fragile glass former. A secondary relaxation is also observed, corresponding to an intramolecular dynamic process of the non-rigid Biclotymol molecule. The crystallization kinetics, measured at four different temperatures above the glass transition temperature, follows an Avrami behavior with exponent virtually equal to n = 2, indicating one-dimensional crystallization into needle-like crystallites, as experimentally observed, with a time-constant nucleation rate. The activation barrier for crystallization is found to be Ea = 115 ± 22 kJ mol(-1).

  6. Thermodynamic clarification of interaction between antiseptic compounds and lipids consisting of stratum corneum

    Energy Technology Data Exchange (ETDEWEB)

    Aki, Hatsumi; Kawasaki, Yuhsuke

    2004-06-24

    The interactions of antiseptic compounds with quaternary ammonium, such as benzalkonium chloride (BC), benzethonium chloride (BZC), dodecyldiaminoethyl-glycine hydrochloride (AEG), and chlorhexidine gluconate (CHG), with components of the stratum corneum were investigated by isothermal titration calorimetry at pH 7.5 and 25 deg. C. The different mechanisms for their permeation to stratum corneum were clarified. Cationic surfactants of BC and BZC bound to cholesterol and cholesterol sulfate with high affinity (10{sup 5}-10{sup 6} M{sup -1}) to extract endogenous cholesterol and its derivatives from the stratum corneum and penetrated via an intercellular route. CHG also bound to cholesterol and accumulated in the stratum corneum without removing endogenous cholesterol. On the other hand, an amphoteric surfactant of AEG seemed to be incorporated into the lipid bilayer and bound to ceramide with its polar end close to the lipid polar heads by hydrophobic interaction.

  7. Anti-septic activity of α-cubebenoate isolated from Schisandra chinensis.

    Science.gov (United States)

    Kook, Minsoo; Lee, Sung Kyun; Kim, Sang Doo; Lee, Ha Young; Hwang, Jae Sam; Choi, Young Whan; Bae, Yoe-Sik

    2015-06-01

    Sepsis is a life-threatening, infectious, systemic inflammatory disease. In this study, we investigated the therapeutic effect of α-cubebenoate, a novel compound isolated from Schisandra chinensis against polymicrobial sepsis in a cecal ligation and puncture (CLP) experimental model. Administration of α-cubebenoate strongly enhanced survival in the CLP model. α-cubebenoate administration also markedly blocked CLP-induced lung inflammation and increased bactericidal activity by enhancing phagocytic activity and hydrogen peroxide generation in mouse bone marrow-derived macrophages and neutrophils. Expression of two important inflammatory cytokines, IL-1β and IL-6, was strongly increased in the CLP model, and this was dramatically blocked by α-cubebenoate. Lymphocyte apoptosis and caspase-3 activation, which are associated with immune paralysis during sepsis, were markedly attenuated by α-cubebenoate. Taken together, our findings indicate that α-cubebenoate, a natural compound isolated from Schisandra chinensis, is a powerful potential anti-septic agent.

  8. N-Chlorotaurine and ammonium chloride: an antiseptic preparation with strong bactericidal activity.

    Science.gov (United States)

    Gottardi, Waldemar; Arnitz, Roland; Nagl, Markus

    2007-04-20

    The bactericidal activity of the endogenous antiseptic N-chlorotaurine (NCT) is significantly enhanced in the presence of ammonium chloride which induces the formation of monochloramine (NH(2)Cl) whose strong bactericidal activity is well known. In this study the properties of NCT plus ammonium chloride have been investigated. The reaction of active chlorine compounds like chloramine-T (N-chlorotoluene-sulfonamide sodium), chloroisocyanuric acid derivatives, hypochlorites (NaOCl, CaOCl(2)) with ammonium chloride did not stop at the stage of monochloramine, and the pungent smelling by-products di- and trichloramine, NHCl(2) and NCl(3), were also formed. This was not the case with NCT where only monochloramine was generated. The equilibrium constant of the reaction of NCT with ammonium was found to be [Formula: see text] , which allows to estimate the equilibrium concentration of monochloramine in aqueous solutions of NCT and ammonium chloride. At concentrations each ranging between 0.01% and 1.0% it comes to [NH(2)Cl]=3.5-254 ppm. As an unexpected result the monochloramine containing formulation turned out to be most stable in plain water without buffer additives. Quantitative killing assays revealed complete inactivation of 10(6) to 10(7)CFU/mL of seven bacterial strains by 0.1% NCT plus 0.1% ammonium chloride within 5 min, while with plain 0.1% NCT an incubation time of 2-4h was needed to achieve the same effect. The highly significant increase of bactericidal activity (200-300-fold) could be assigned to the presence of monochloramine which could be isolated by vacuum distillation. Aqueous solutions of NCT and ammonium chloride provide a highly effective and well tolerable antiseptic preparation appropriate to a treatment cycle of at least 1 month if stored in the refrigerator.

  9. Cetylpyridinium chloride and miramistin as antiseptic substances in chronic wound management - prospects and limitations.

    Science.gov (United States)

    Fromm-Dornieden, Carolin; Rembe, Julian-Dario; Schäfer, Nadine; Böhm, Julia; Stuermer, Ewa K

    2015-04-01

    The antimicrobial activity of cetylpyridinium chloride (CPC) and miramistin (MST) solutions at different concentrations (5×10(-5) to 0.4%) and a dressing, containing 0.15% CPC, were tested against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli after 30 (solutions) and 60 min (fleece) incubation, respectively. Furthermore, the cytotoxic effects of CPC and MST were examined in human keratinocyte (HaCaT) and murine fibroblast (L929) cell lines. A dose of 3×10(-3)% CPC or MST was sufficient to entirely eradicate S. aureus after 30 min incubation. To achieve the same effect, higher concentrations were required against E. coli (0.025% CPC; 0.0125% MST) and P. aeruginosa (0.5% CPC; 0.05% MST). The CPC-fleece showed a high antiseptic effect against all three bacterial strains, although it did not completely eliminate P. aeruginosa. Both substances showed a high cytotoxic impact at higher tested concentrations (CPC >3×10(-3)%; MST >8×10(-4)%). CPC showed high antimicrobial potency at low concentrations against S. aureus, accompanied by low cytotoxic (side) effects at these concentrations, whilst the required minimal concentration to eradicate E. coli and P. aeruginosa was shown to be cytotoxic for keratinocytes and fibroblasts. The necessary antibacterial amounts of MST were lower, but also cytotoxic in direct contact with typical human wound cells. With regard to demographic changes and increasing bacterial resistance, new effective antiseptics, such as CPC and MST, incorporated in wound dressings without releasing an active substance could help to improve the treatment and healing rates of chronic wounds.

  10. [Effects of antiseptic on the analysis of greenhouse gases concentrations in lake water].

    Science.gov (United States)

    Xiao, Qi-Tao; Hu, Zheng-Hu; James, Deng; Xiao, Wei; Liu, Shou-Dong; Li, Xu-Hui

    2014-01-01

    To gain insight into antiseptic effects on the concentrations of CO2, CH4, and N2O in lake water, antisepetic (CuSO4 and HgCl2) were added into water sample, and concentrations of greenhouse gases were measured by the gas chromatography based on water equilibrium method. Experiments were conducted as following: the control group without antisepetic (CK), the treatment group with 1 mL CuSO4 solution (T1), the treatment group with 5 mL CuSO4 solution (T2), and the treatment group with 0.5 mL HgCl2 solution (T3). All groups were divided into two batches: immediately analysis (I), and after 2 days analysis (II). Results showed that CuSO4 and HgCl2 significantly increased CO2 concentration, the mean CO2 concentration (Mco2) of CK (I) and CK (II) were (11.5 +/- 1.47) micromol x L(-1) and (14.38 +/- 1.59) micromol x L(-1), respectively; the Mco2 of T1 (I) and T1 (II) were (376 +/- 70) micromol x L(-1) and (448 +/- 246.83) micromol x L(-1), respectively; the Mco2 of T2 (I) and T2 (II) were (885 +/- 51.53) micromol x L(-1) and (988.83 +/- 101.96) micromol x L(-1), respectively; the Mco2 of T3 (I) and T3 (II) were (287.19 +/- 30.01) micromol x L(-1) and (331.33 +/- 22.06) micromol x L(-1), respectively. The results also showed that there was no difference in CH4 and N2O concentrations among treatments. Water samples should be analyzed as soon as possible after pretreatment. Our findings suggest that adding antiseptic may lead an increase in CO2 concentration.

  11. ASSESSMENT OF THE EFFECTIVENESS OF APPLICATION ANTISEPTICS IN PREVENTION OF FOOT SKIN INFLAMMATION

    Directory of Open Access Journals (Sweden)

    Brzeziński Piotr

    2011-01-01

    Full Text Available Introduction: Skin inflammation in the foot soldiers is a major problem, affecting armies around the world. Hence it is important preventive action, to prevent the development of such states. Antiseptic is the destruction of microorganisms present in the tissues by means of antiseptic.Aim: The effectiveness of the use Pigmentum Castelani and Kalium hypermanganicum in the prevention of skin inflammation in the foot soldiers.Material and methods: The study included 30 men middle-aged men 20.5 years and 60 soldiers, as a control group of men with a mean age 21.5 years. The survey was conducted over a period of 3 months. Of the 30 soldiers from the study group in 15 using liquid Pigmentum Castelani - Group I, and in another 15 people a solution of Kalium hypermanganicum - group II. Prophylactic treatment of soldiers received by a certain pattern. The control group was 60 men received no treatment. The observations were performed during clinical examination of foot skin, mycological examination and examination under Wood's lamp.Results: None of the 30 seamen (group I and II test, there was no bacterial or fungal infection of the feet. In the control group were diagnosed 11 cases of athlete's foot feet (64.71% of cases, 3 cases of fungal infection of the back foot (17.65% and 3 cases pitted keratolysis (17.65% of cases.Conclusions: Prophylactic Pigmentum Castelani and Kalium hypermanganicum effectively protects against the development of inflammatory skin feet. This is reflected in the increase in disposal and good health for the soldier.

  12. The effects of biocides (antiseptics and disinfectants on the endospores of Rhinosporidium seeberi

    Directory of Open Access Journals (Sweden)

    Arseculeratne S

    2006-01-01

    Full Text Available No data exists on the activity of biocides (antiseptics and disinfectants on Rhinosporidium seeberi that causes rhinosporidiosis in humans and animals. On account of the inability to culture R. seeberi, in vitro , dyes were used to assess the morphological integrity and viability of biocide-treated endospores that are considered to be the infective stage of this pathogen. Evan′s Blue (EvB identifies the morphological integrity of the endospores while MTT (3-[4, 5-dimethylthiazol-2yl]-2, 5-diphenyl tetrazolium bromide identifies metabolic activity through its reduction by cellular dehydrogenases to microscopically visible deposits of insoluble formazan. MTT-negativity has earlier been shown to correlate with absence of growth of yeast and mycelial fungi in culture and could thus indicate the loss of viability of MTT-negative rhinosporidial endospores. Hydrogen peroxide, glutaraldehyde, chloroxylenol, chlorhexidine, cetrimide, thimerosal, 70% ethanol, iodine in 70% ethanol, 10% formalin, povidone-iodine, sodium azide and silver nitrate were tested on freshly-harvested endospores and all biocides caused metabolic inactivation with or without altered structural integrity as shown by absence of MTT-staining after 3, 24 or 36 hour after exposure, while EvB stained only the endospores treated with sodium azide, ethanol, thimerosal, chloroxylenol, glutaraldehyde and hydrogen peroxide. With clinically useful biocides - chlorhexidine, cetrimide-chlorhexidine, 70% ethanol, povidone-iodine and silver nitrate, a total period of exposure of endospores to the biocide, for seven minutes, produced metabolic inactivation of the endospores. Anti-rhinosporidial antiseptics that could be used in surgery on rhinosporidial patients include povidone-iodine in nasal packs for nasal and naso-pharyngeal surgery, chlorhexidine and cetrimide-chlorhexidine on the skin, while povidone-iodine and silver nitrate could have application in ocular rhinosporidiosis.

  13. Estimated hospital costs associated with preventable health care-associated infections if health care antiseptic products were unavailable

    Directory of Open Access Journals (Sweden)

    Schmier JK

    2016-05-01

    Full Text Available Jordana K Schmier,1 Carolyn K Hulme-Lowe,1 Svetlana Semenova,2 Juergen A Klenk,3 Paul C DeLeo,4 Richard Sedlak,5 Pete A Carlson6 1Health Sciences, Exponent, Inc., Alexandria, VA, 2EcoSciences, Exponent, Inc., Maynard, MA, 3Health Sciences, Exponent, Inc., Alexandria, VA, 4Environmental Safety, 5Technical and International Affairs, American Cleaning Institute, Washington, DC, 6Regulatory Affairs, Ecolab, Saint Paul, MN, USA Objectives: Health care-associated infections (HAIs pose a significant health care and cost burden. This study estimates annual HAI hospital costs in the US avoided through use of health care antiseptics (health care personnel hand washes and rubs; surgical hand scrubs and rubs; patient preoperative and preinjection skin preparations. Methods: A spreadsheet model was developed with base case inputs derived from the published literature, supplemented with assumptions when data were insufficient. Five HAIs of interest were identified: catheter-associated urinary tract infections, central line-associated bloodstream infections, gastrointestinal infections caused by Clostridium difficile, hospital- or ventilator-associated pneumonia, and surgical site infections. A national estimate of the annual potential lost benefits from elimination of these products is calculated based on the number of HAIs, the proportion of HAIs that are preventable, the proportion of preventable HAIs associated with health care antiseptics, and HAI hospital costs. The model is designed to be user friendly and to allow assumptions about prevention across all infections to vary or stay the same. Sensitivity analyses provide low- and high-end estimates of costs avoided. Results: Low- and high-end estimates of national, annual HAIs in hospitals avoided through use of health care antiseptics are 12,100 and 223,000, respectively, with associated hospital costs avoided of US$142 million and US$4.25 billion, respectively. Conclusion: The model presents a novel

  14. Extremely high prevalence of antiseptic resistant Quaternary Ammonium Compound E gene among clinical isolates of multiple drug resistant Acinetobacter baumannii in Malaysia

    OpenAIRE

    Babaei, Mohammad Reza; Sulong, Anita; Hamat, Rukman Awang; Nordin, Syafinaz Amin; Neela, Vasantha Kumari

    2015-01-01

    Background Antiseptics are commonly used for the management of MDR (multiple drug resistance) pathogens in hospitals. They play crucial roles in the infection control practices. Antiseptics are often used for skin antisepsis, gauze dressing, preparation of anatomical sites for surgical procedure, hand sterilization before in contact with an infected person, before an invasive procedure and as surgical scrub. Methods We screened 122 multiple drug resistant Acinetobacter baumannii (MDRAB) isola...

  15. Search for microorganisms in antiseptic, disinfectant and detergent dispensers of a local health care unit from Northeast Portugal

    OpenAIRE

    Alves, Maria José; Pombo, Graça; Pereira, Ana; Conde, Andreia; Soares, Isabel; Rosmaninho, Marina; Ferreira, Isabel C. F. R.

    2015-01-01

    Introduction: Health care associated infections (HCAI) are among the main causes of mortality worldwide, and of the increase of internment time and related costs. Many HCAI acquired in hospitals result from improper disinfection practices of medical devices and surfaces of the hospital environment, as also insufficient asepsis of health professional’s skin [1,2]. Objectives: The main objectives of the present work were to verify the presence of microorganisms in antiseptic, ...

  16. Evaluation of the tissue toxicity of antiseptics by the hen's egg test on the chorioallantoic membrane (HETCAM

    Directory of Open Access Journals (Sweden)

    Marquardt C

    2010-05-01

    Full Text Available Abstract Background Antiseptics are frequently used for the prophylaxis and treatment of local infections of chronic wounds. Whereas local antiseptics in general have a positive effect on wound healing an uncritical use may impair wound healing due to toxic side effects. Objective We sought to assess the vascular irritation potential of different antiseptic solutions and ointments commonly used for short and long term application as a measure of tissue toxicity. Method The vascular irritation was evaluated by the hen's egg test (HET on the chorioallantoic membrane (CAM. The effects on the vessels of a mucous membrane were directly assessed by stereomicroscopic observation in vivo. Results Severe CAM irritation was observed after short-term applications of 1% octenidin-2HCl (Octeni sept™, 72% isopropanol (Cutasept™, 0.35% chloroxylenol (Dettol™ and 10% PVP-I ointment (Betaisodona™. Medium irritations were observed for 10% PVP-I solution (Betaisodona™, 3% lysosomal PVP-I ointment (Repithel™, 1.8% cadexomer-iodine ointment (Iodosorb™ and 1% cadexomer-iodine pellets (Iodosorb™. Finally, slight irritations were observed for 1% PVP-I solution (Betaisodona™, 0.1% polyhexanid plus betain (Prontosan™ and 1% silver-sulfadiazine ointment (Flammazine™, whereas 0.04% polyhexanid solution (Lavanid™, washings from sterile maggots of Lucilia sericata and filtrated enzymes from Clostridium histolyticum (Iruxol-N™ showed no effects of irritation. In the long-term approaches, no vascular irritations were found for polyhexanid, washings from Lucilia sericata and enzyme filtrations from Clostridium histolyticum. Conclusion The vascular injuries caused by the studied antiseptics are an indirect indicator of their tissue toxicity. Strikingly, even therapeutic substances, which have been regarded as safe in their application for the treatment of chronic wounds in clinical studies, showed severe irritations on the CAM. We suggest that agents

  17. Human coronaviruses: insights into environmental resistance and its influence on the development of new antiseptic strategies.

    Science.gov (United States)

    Geller, Chloé; Varbanov, Mihayl; Duval, Raphaël E

    2012-11-12

    environment and the efficacy of well-known antiseptic-disinfectants against them, with particular focus on the development of new methodologies to evaluate the activity of new antiseptic-disinfectants on viruses.

  18. Partitioning, persistence, and accumulation in digested sludge of the topical antiseptic triclocarban during wastewater treatment.

    Science.gov (United States)

    Heidler, Jochen; Sapkota, Amir; Halden, Rolf U

    2006-06-01

    The topical antiseptic agent triclocarban (TCC) is a common additive in many antimicrobial household consumables, including soaps and other personal care products. Long-term usage of the mass-produced compound and a lack of understanding of its fate during sewage treatment motivated the present mass balance analysis conducted at a typical U.S. activated sludge wastewater treatment plant featuring a design capacity of 680 million liters per day. Using automated samplers and grab sampling, the mass of TCC contained in influent, effluent, and digested sludge was monitored by isotope dilution liquid chromatography (tandem) mass spectrometry. The average mass of TCC (mean +/- standard deviation) entering and exiting the plant in influent (6.1 +/- 2.0 microg/L) and effluent (0.17 +/- 0.03 microg/ L) was 3737 +/- 694 and 127 +/- 6 g/d, respectively, indicating an aqueous-phase removal efficiency of 97 +/- 1%. Tertiary treatment by chlorination and sand filtration provided no detectable benefit to the overall removal. Due to strong sorption of TCC to wastewater particulate matter (78 +/- 11% sorbed), the majority of the TCC mass was sequestered into sludge in the primary and secondary clarifiers of the plant. Anaerobic digestion for 19 days did not promote TCC transformation, resulting in an accumulation of the antiseptic compound in dewatered, digested municipal sludge to levels of 51 +/- 15 mg/kg dry weight (2815 +/- 917 g/d). In addition to the biocide mass passing through the plant contained in the effluent (3 +/- 1%), 76 +/- 30% of the TCC input entering the plant underwent no net transformation and instead partitioned into and accumulated in municipal sludge. Based on the rate of beneficial reuse of sludge produced by this facility (95%), which exceeds the national average (63%), study results suggest that approximately three-quarters of the mass of TCC disposed of by consumers in the sewershed of the plant ultimately is released into the environment by application

  19. Synergistic antibiofilm efficacy of various commercial antiseptics, enzymes and EDTA: a study of Pseudomonas aeruginosa and Staphylococcus aureus biofilms.

    Science.gov (United States)

    Lefebvre, Elodie; Vighetto, Christophe; Di Martino, Patrick; Larreta Garde, Véronique; Seyer, Damien

    2016-08-01

    A multistep strategy was used to generate a combined antibiofilm treatment that could efficiently decrease the biomass of dense biofilms (≥6 × 10(7) CFU/cm(2)). Several compounds that exhibited activity against various targets were tested individually and in combination to search for possible synergistic effects. First, the antibiofilm activity of various commercially available antiseptics was tested on Pseudomonas aeruginosa and Staphylococcus aureus. Second, antiseptics were mixed with ethylene diamine tetra-acetic acid (EDTA), which is an ion chelator that can disturb biofilm organisation, and additive effects on biofilm biomass degradation were found for both strains. Then, enzymes with the ability to destabilise the biofilm matrix by hydrolysing either its proteins or its polysaccharides were used; as expected, they did not decrease bacterial viability but were revealed as efficient biomass reducers. The combination of antiseptics, EDTA and proteases, all at low concentrations, revealed a synergistic effect leading to total eradication of dense biofilms both of P. aeruginosa and S. aureus. PMID:27424598

  20. Orthopaedic metal devices coated with a novel antiseptic dye for the prevention of bacterial infections.

    Science.gov (United States)

    Bahna, Paul; Dvorak, Tanya; Hanna, Hend; Yasko, Alan W; Hachem, Ray; Raad, Issam

    2007-05-01

    Gendine is a novel antiseptic dye with broad-spectrum antimicrobial activity that may be used to coat plastics and metal devices. Our objective was to determine the efficacy of gendine-coated orthopaedic metal devices in preventing methicillin-resistant Staphylococcus aureus (MRSA) colonisation. Stainless steel and titanium Schanz rods were coated with gendine. The zone of inhibition (ZoI) around the rods with and without gamma-irradiation was determined by a modified Kirby-Bauer method. A previously published bioprosthetic biofilm colonisation model, modified Kuhn's method, was used to determine the adherence of MRSA to coated and uncoated rods, with and without irradiation, after insertion into bovine bone and after 3 months shelf life followed by 2 weeks of immersion in serum. The gendine-coated Schanz metal rods showed a net ZoI of 16 mm against MRSA before and after irradiation. Gendine-coated rods showed no biofilm formation (0 colony-forming units (CFU)), which was a significant reduction (P5000 CFU). Coated rods exposed to high-dose gamma-irradiation and coated rods drilled into bone also showed significant efficacy (P<0.001) in preventing biofilm adherence. After 2 weeks, gendine-coated rods maintained significant durability (P<0.01), resulting in 90% reduction in MRSA biofilm adherence compared with uncoated control rods. Results indicate that gendine-coated metal rods are highly efficacious in the prevention of MRSA biofilm.

  1. Evaluation Clinical Effects of Anti-Septic Solution Based on Propolis in Caries-Active Children

    Directory of Open Access Journals (Sweden)

    Rossana Vanessa Dantas de ALMEIDA

    2006-04-01

    Full Text Available Objective: This study aimed validate the action of one anti-septic solution based on propolis against the clinical indexes and levels of S. mutans. Method: The antimicrobial activity of the extract was done on solid plates to determinate the Minimum Inhibitory Concentration (MIC using sorts of S. mutans. With the MIC of the extract, was made a mouthrinse solution of propolis (6.25%, which was used clinically as a test solution and compared with positive control solution, the chlorexidine (0.12%. Through the crossover design, fifteen children used the mouthrinse solution of propolis during 15 consecutive days and after a break of 21 days, they used daily mouthrinse with the chlorexidine. Were collected indexes for accumulation of oral biofilm (OHI-S and for gun disease (GBI before (T0 and 24 hours after (T1 the use of the solutions, besides of the couting of the S. mutans from saliva's samples before (T0 and 24 hours (T0, 7 days (T7, 15 days (T15 e 21 days (T21 after the end of both mouthrinses. Results: The results demonstrated significative reduction of the S. mutans levels 24 hours (p0.05. Conclusion: The propolis extract showed satisfactory antimicrobial activity and close to the chlorexidine's action, besides of act against clinical conditions of oral biofilm's presence and gun disease what becomes it useful as therapeutic agent.

  2. SENSITIVITY TO ANTIBIOTICS, ANTISEPTICAL NOSOCOMIAL PSEUDOMONAS AERUGINOSA, ISOLATED IN UROLOGICAL PATIENTS

    Directory of Open Access Journals (Sweden)

    Rymsha E.V.

    2015-05-01

    Full Text Available Introduction. Given the active introduction into clinical practice of new groups of antibiotics and antiseptics, the problem of treatment of purulent-inflammatory complications after prostatectomy and today is relevant. Of particular concern belated cases of diagnosis and treatment of postoperative complications in urological practice patients receiving antibiotic therapy The use of traditional antibiotics is not prevents the development of infection, because the problem of resistance of microorganisms to antibiotics and antiseptics remains relevant. The solution to the problem of development of infectious complications and prevent the formation of resistant clinical strains largely depends on the isolated pathogen, susceptibility to antimicrobial agents based on its bioavailability , ability to spread and penetrate into cells and tissues, selection of dose, interval, and route of administration to maintain minimum bactericidal concentration Material and methods. The study involved 145 patients who were treated in the urology Department of the Vinnytsia regional clinical hospital named of M. I. Pirogov. Patients underwent the surgical treatment of benign hypertrophic prostate. Material for bacteriological studies of purulent-inflammatory diseases were urine, pieces of the prostate, remote operationally, urinary catheters, through which conducted irrigation of the bladder. Specimen collection, transportation was carried out in accordance with modern requirements. Identification was done by morphological, cultural and biochemical properties. The definition of antibiotic resistance were performed according to "guidelines for the definition of sensitivity of microorganisms to antibiotics by the method of diffusion in agar using discs" (No. 2675-83, Kiev, 2007 12 .]. Evaluation of the results of determining the sensitivity of microorganisms to antibiotics was carried out on the basis of the determination of the zone of growth (mm of the studied

  3. Quantitative Analysis of Single and Mix Food Antiseptics Basing on SERS Spectra with PLSR Method.

    Science.gov (United States)

    Hou, Mengjing; Huang, Yu; Ma, Lingwei; Zhang, Zhengjun

    2016-12-01

    Usage and dosage of food antiseptics are very concerned due to their decisive influence in food safety. Surface-enhanced Raman scattering (SERS) effect was employed in this research to realize trace potassium sorbate (PS) and sodium benzoate (SB) detection. HfO2 ultrathin film-coated Ag NR array was fabricated as SERS substrate. Protected by HfO2 film, the SERS substrate possesses good acid resistance, which enables it to be applicable in acidic environment where PS and SB work. Regression relationship between SERS spectra of 0.3~10 mg/L PS solution and their concentration was calibrated by partial least squares regression (PLSR) method, and the concentration prediction performance was quite satisfactory. Furthermore, mixture solution of PS and SB was also quantitatively analyzed by PLSR method. Spectrum data of characteristic peak sections corresponding to PS and SB was used to establish the regression models of these two solutes, respectively, and their concentrations were determined accurately despite their characteristic peak sections overlapping. It is possible that the unique modeling process of PLSR method prevented the overlapped Raman signal from reducing the model accuracy. PMID:27299651

  4. Quantitative Analysis of Single and Mix Food Antiseptics Basing on SERS Spectra with PLSR Method

    Science.gov (United States)

    Hou, Mengjing; Huang, Yu; Ma, Lingwei; Zhang, Zhengjun

    2016-06-01

    Usage and dosage of food antiseptics are very concerned due to their decisive influence in food safety. Surface-enhanced Raman scattering (SERS) effect was employed in this research to realize trace potassium sorbate (PS) and sodium benzoate (SB) detection. HfO2 ultrathin film-coated Ag NR array was fabricated as SERS substrate. Protected by HfO2 film, the SERS substrate possesses good acid resistance, which enables it to be applicable in acidic environment where PS and SB work. Regression relationship between SERS spectra of 0.3~10 mg/L PS solution and their concentration was calibrated by partial least squares regression (PLSR) method, and the concentration prediction performance was quite satisfactory. Furthermore, mixture solution of PS and SB was also quantitatively analyzed by PLSR method. Spectrum data of characteristic peak sections corresponding to PS and SB was used to establish the regression models of these two solutes, respectively, and their concentrations were determined accurately despite their characteristic peak sections overlapping. It is possible that the unique modeling process of PLSR method prevented the overlapped Raman signal from reducing the model accuracy.

  5. An antiseptic religion: discovering a hybridity on the flux of hygiene and Christianity.

    Science.gov (United States)

    Kim, Shin K

    2008-06-01

    An antiseptic religion is a form of Protestant Christianity that was shaped in the context of colonization in Korea. This term was coined to explain a religious hybrid that was produced by the intermingling of American Evangelical Protestant Christianity, the concept of hygiene (germ theory) and indigenous Korean religiosity. This research deals with a historical process of making 'a medicalized religion' in Asia from a perspective of postcolonialism. Most of the early American Protestant missionaries in Korea were medical doctors who were influenced by the Germ theory of illness and considered Western medicine as an efficient tool to evangelize the country. As a result of their mission, a religious culture which emphasized washing away sins from the soul as analogous to washing away germs from the body was born. In addition, the Korean people developed a very unique form of public bathing ritual centered in the development of public baths to alleviate anxiety and to destabilize the solid strategies of the Japanese and the Americans, the two major colonial powers in Korea's history in the late 19th century. PMID:19105015

  6. 药物制剂防腐剂概述%A Overview of Antiseptics for Pharmaceutical Preparations

    Institute of Scientific and Technical Information of China (English)

    欧阳; 灵莉

    2015-01-01

    Objective:To learn the application of additives ,mainly antiseptics ,so as to learn its safe and rational application.Methods:Collect,view,file up and analyze document literature at home and abroad related to such problem.Results:The application of drug antiseptics is researched ,developed and manufactured based on the standard which were set based on the research achievement at home and abroad and a large part of it is clinical drug and there is medical criteria for it,and there are criteria of various level for the manufacture of other antiseptics and there are scientific bases for them .Conclusion:Although a lot of researches and im-provement still wait to be done for antiseptics which are taken as additives ,without drug antiseptics,it is im-possible to be called modern pharmacy industry.%目的::了解添加剂,主要是防腐剂的应用,以了解其使用的安全和合理性。方法:收集国内讨论此问题的文献资料进行阅读、整理和分析。结果:药品防腐剂的使用是借鉴国内外的研究成果制订的标准而进行研究、开发和生产的、它的相当部分也是临床使用的药品,有药用标准,其他防腐剂的生产也制订有各种级别的标准,是有科学依据的。结论:虽然作为添加剂的防腐剂尚有许多需要研究完善的空间,但没有药品防腐剂是不能成其为现代的的制药工业。

  7. Biocompatibility and other properties of acrylic bone cements prepared with antiseptic activators.

    Science.gov (United States)

    de la Torre, B; Fernández, M; Vázquez, B; Collía, F; de Pedro, J A; López-Bravo, A; San Román, J

    2003-08-15

    Acrylic bone cements prepared with activators of reduced toxicity have been formulated with the aim of improving the biocompatibility of the final material. The activators used were N,N-dimethylaminobenzyl alcohol (DMOH) and 4,4'-dimethylamino benzydrol (BZN). The toxicity, cytotoxicity, and antiseptic action of these activators were first studied. DMOH and BZN presented LD50 values 3-4 times higher than DMT, were less cytotoxic against polymorphonuclear leucocytes, and possessed an antimicrobial character, with a high activity against the most representative microorganisms involved in postoperative infections. The properties of the acrylic bone cements formulated with DMOH and BZN were evaluated to determine the influence of these activators on the curing process and the physicochemical characteristics of the cements. A decrease of the peak temperature was observed for the curing with DMOH or BZN with respect to that of one commercially available formulation (CMW 3). However, residual monomer content and mechanical properties in tension and compression were comparable to those of CMW 3. The biocompatibility of acrylic bone cements containing DMOH or BZN was studied and compared with CMW 3. To that end, intramuscular and intraosseous implantation procedures were carried out and the results were obtained from the histological analysis of the surrounding tissues at different periods of time. Implantation of rods of cement into the dorsal muscle of rats showed the presence of a membrane of connective tissue, which increased in collagen fibers with time of implantation, for all formulations. The intraosseous implantation of the cements in the dough state in the femur of rabbits, revealed a higher and early osseous neoformation, with the presence of osteoid material surrounding the rest of the cured material, for the cement prepared with the activator BZN in comparison with that obtained following the implantation of the cement cured with DMOH or DMT (CMW 3).

  8. Bactericidal Effects and Mechanism of Action of Olanexidine Gluconate, a New Antiseptic.

    Science.gov (United States)

    Hagi, Akifumi; Iwata, Koushi; Nii, Takuya; Nakata, Hikaru; Tsubotani, Yoshie; Inoue, Yasuhide

    2015-08-01

    Olanexidine gluconate [1-(3,4-dichlorobenzyl)-5-octylbiguanide gluconate] (development code OPB-2045G) is a new monobiguanide compound with bactericidal activity. In this study, we assessed its spectrum of bactericidal activity and mechanism of action. The minimal bactericidal concentrations of the compound for 30-, 60-, and 180-s exposures were determined with the microdilution method using a neutralizer against 320 bacterial strains from culture collections and clinical isolates. Based on the results, the estimated bactericidal olanexidine concentrations with 180-s exposures were 869 μg/ml for Gram-positive cocci (155 strains), 109 μg/ml for Gram-positive bacilli (29 strains), and 434 μg/ml for Gram-negative bacteria (136 strains). Olanexidine was active against a wide range of bacteria, especially Gram-positive cocci, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci, and had a spectrum of bactericidal activity comparable to that of commercial antiseptics, such as chlorhexidine and povidone-iodine. In vitro experiments exploring its mechanism of action indicated that olanexidine (i) interacts with the bacterial surface molecules, such as lipopolysaccharide and lipoteichoic acid, (ii) disrupts the cell membranes of liposomes, which are artificial bacterial membrane models, (iii) enhances the membrane permeability of Escherichia coli, (iv) disrupts the membrane integrity of S. aureus, and (v) denatures proteins at relatively high concentrations (≥160 μg/ml). These results indicate that olanexidine probably binds to the cell membrane, disrupts membrane integrity, and its bacteriostatic and bactericidal effects are caused by irreversible leakage of intracellular components. At relatively high concentrations, olanexidine aggregates cells by denaturing proteins. This mechanism differs slightly from that of a similar biguanide compound, chlorhexidine.

  9. Antiseptic effect of a novel alcohol-free mouthwash: a convenient prophylactic alternative for high-risk patients.

    Science.gov (United States)

    Bahna, Paul; Hanna, Hend A; Dvorak, Tanya; Vaporciyan, Ara; Chambers, Mark; Raad, Issam

    2007-02-01

    We developed an efficacious and non-irritant mouthwash that is alcohol-free and that has a low concentration of chlorhexidine, in order to be used for preventing oral cavity infections in immunocompromised and cancer patients. The novel mouthwash solution was tested for its antimicrobial efficacy against both free floating (planktonic) and the biofilm forms of Candida albicans. The solution was also tested against Klebsiella pneumoniae, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus (MRSA), using a modification of a previously published method. The activity of the novel mouthwash was also compared with that of three commercially available mouthwashes. The experimental mouthwash showed efficacy against C. albicans, both in free-floating form and in biofilm. With higher concentration of chlorhexidine, the solution was also efficacious in inhibiting the growth of K. pneumoniae, P. aeruginosa, and MRSA. The antiseptic activity of the alcohol-free mouthwash against other bacterial organisms and C. albicans was comparable to other commercially available alcohol-based mouthwash solutions. A novel alcohol-free mouthwash solution, that has low concentration of chlorhexidine, showed antiseptic effect against planktonic and biofilm forms of C. albicans and against K. pneumoniae, P. aeruginosa, and MRSA.

  10. Study on antiseptic effect of curcumin on meat products%姜黄色素对肉制品的防腐作用研究

    Institute of Scientific and Technical Information of China (English)

    雷昌贵; 孟宇竹

    2012-01-01

    本试验以五香牛肉、西式香肠、酱卤肉、腊肉四种具有代表性的肉制品为供试材料,探讨姜黄色素对肉制品的防腐作用。结果表明:浓度在0.04%以上的姜黄色素碱水溶液对西式香肠、腊肉具有良好的防腐性;浓度在0.06%以上的姜黄色素碱水溶液对五香牛肉、酱卤肉具有较好的防腐性,姜黄色素在加热煮沸的条件下失去防腐功效。%Spiced beef, western sausage, sauced meat, Chinese bacon were taken as experimental materials to study antiseptic effect of curcumin in this paper. The results showed that curcumin solution had good antiseptic effect on western sausage and Chinese bacon when the concentration was above 0. 04%, and it had good antiseptic effect on spiced beef and cooked meat when the concentration was above 0. 06%. But the cureumin had no antiseptic effect at all after boiled.

  11. 激素和防腐剂对树木损伤修复技术研究%Studies Technology on both Hormone and Antiseptic Renovating Trees Injury

    Institute of Scientific and Technical Information of China (English)

    王晓英; 冯殿齐; 王玉山; 赵进红; 陶娟; 高尚坤

    2012-01-01

    对5种树种的树干伤及形成层情况下,设置5个伤口涂补处理:1.空白,2.防腐剂,3.6-BA(5ppm)+防腐剂,4.IAA(15ppm)+防腐剂,5.6-BA(5ppm)+IAA(15ppm)+防腐剂。结果表明,防腐剂一纯松烟墨汁可以有效防止病原微生物侵染伤口和树体腐朽。涂补激素和防腐剂处理3、4、5与只涂补防腐剂处理2相比,添加激素涂补明显加速了伤口愈合,而处理3、4、5之间未见显著差异。激素并口墨汁溶液可以作为新型树木伤口液体涂料,具有一定的开发价值。%In the cambium injury of trunk of 5 tree species, the experiment seted up five wounds smearing treatments: 1. Blank, 2. Antiseptic, 3. 6--BA (5 ppm) and antiseptic, 4. IAA (15 ppm) and antiseptic, 5. 6--BA (5 ppm), IAA (15 ppm) and antiseptic. The results showed that the antiseptic--pure SongYan ink could effectively prevent the pathogenic microbes from infecting the wound and tree trunk decayed. Compare smearing hormone and antiseptic treatments 3, 4, 5 with only smea- ring antiseptic treatment 2, adding hormone significantly accelerated the wound healing, and there were no significantly differ- ences between 3, 4, and 5 treatments. Hormone and ink solution can be used as a new tree would liquid coating products, and it has some of the development value.

  12. OBSERVATION ON SKIN AND MUCOSA IRRITATION OF A KUSHEN ANTISEPTIC LOTION%苦参抑菌洗液皮肤和黏膜刺激性观察

    Institute of Scientific and Technical Information of China (English)

    张在平; 尹幸念; 王莉; 钱毅春; 樊树理; 解国梁

    2012-01-01

    Objective To observe the irritation of the Kushen antiseptic lotion on skin and mucosa in order to supply basis for administration of safe use. Methods Animal experiment was used to observe the irritation of the Kushen antiseptic lotion on rabbits' skin and vaginal mucosa. Results The score of skin irritation reaction was 0 when rabbits'back skin contacted with the Kushen antiseptic stock lotion for many times; the irritating index of vaginal mucosa was 0. 45 when the rabbits'vaginal mucosa contacted with the same concentration of the Kushen antiseptic lotion for many times. Conclusion The Kushen antiseptic lotion has no obvious irritation effect on rabbits' skin and vaginal mucosa.%目的 观察苦参抑菌洗液对皮肤和黏膜的刺激性,为安全使用提供依据.方法 通过动物试验法,观察了苦参抑菌洗液对家兔皮肤和阴道黏膜刺激性.结果 用苦参抑菌洗液原液与家兔背部皮肤多次接触试验,皮肤刺激反应积分为0.同样浓度该洗液多次接触家兔阴道黏膜,刺激指数为0.45.结论 苦参抑菌洗液对家兔皮肤及阴道黏膜组织均无刺激性.

  13. Enrichment, development, and assessment of Indian basil oil based antiseptic cream formulation utilizing hydrophilic-lipophilic balance approach.

    Science.gov (United States)

    Yadav, Narayan Prasad; Meher, Jaya Gopal; Pandey, Neelam; Luqman, Suaib; Yadav, Kuldeep Singh; Chanda, Debabrata

    2013-01-01

    The present work was aimed to develop an antiseptic cream formulation of Indian basil oil utilizing hydrophilic-lipophilic balance approach. In order to determine the required-hydrophilic lipophilic balance (rHLB) of basil oil, emulsions of basil oil were prepared by phase inversion temperature technique using water, Tween 80, and Span 80. Formulated emulsions were assessed for creaming (BE9; 9.8, BE10; 10.2), droplet size (BE18; 3.22 ± 0.09 μ m), and turbidity (BE18; 86.12 ± 2.1%). To ensure correctness of the applied methodology, rHLB of light liquid paraffin was also determined. After rHLB determination, basil oil creams were prepared with two different combinations of surfactants, namely, GMS : Tween 80 (1 : 3.45) and SLS : GMS (1 : 3.68), and evaluated for in vitro antimicrobial activity, skin irritation test, viscosity and consistency. The rHLB of basil oil and light liquid paraffin were found to be 13.36 ± 0.36 and 11.5 ± 0.35, respectively. Viscosity, and consistency parameters of cream was found to be consistent over 90 days. Cream formulations showed net zone of growth inhibition in the range of 5.0-11.3 mm against bacteria and 4.3-7.6 mm against fungi. Primary irritation index was found to be between 0.38 and1.05. Conclusively stable, consistent, non-irritant, enriched antiseptic basil oil cream formulations were developed utilizing HLB approach.

  14. Evaluation of inactivation of intracanal antiseptics by dentin, demineralized dentin, dentin matrix and mineral component of dentin

    Directory of Open Access Journals (Sweden)

    Razmi H

    2007-01-01

    Full Text Available Background and Aim: Many studies have shown that microorganisms are the main cause of pulpal diseases and the main purpose of root canal therapy is their elimination from the root canal system. Antiseptic agents are used to reduce bacteria but their antibacterial activities differ from in vivo to in vitro studies and might be inactivated by dentin and its components in root canal space. This study was designed to investigate the effect of dentin on antibacterial activity of different antimicrobial agents. Materials and Methods: In this experimental study, two antibacterial agents (sodium hypochlorite and chlorhexidine with different concentrations were used in four experimental groups: Group 1: dentin, Group 2: demineralized dentin with EDTA, Group 3: dentin matrix and Group 4: dentin mineral component. The species used in this study was Entrococcus faecalis. Different concentration of agents were added to mixture of each experimental group and bacteria. At the baseline and after one and 24 hours, samples were collected and cultured. After incubation period, colonies were counted. Data were analyzed by Tukey test with p<0.05 as the limit of significance. Results: 2% and 0.2% chlorhexidine, and 5% sodium hypochlorite solutions at the three studied times eliminated Entrococcus faecalis completely. 1% sodium hypochlorite eliminated all bacteria in 1h and 24 hs. Statistical analysis showed significant differences between experimental and control groups (P<0.05. Sodium 1% hypochlorite at time 0, could reduce bacteria significantly (P<0.05 but didn’t eliminate them completely. Conclusion: Inactivation of intracanal antiseptics was not observed in this study. As elimination of bacteria occurred, application of these antibacterial agents are recommended in endodontic treatment. Further investigations on other antibacterial agents, other concentrations and shorter time intervals are recommended.

  15. 术前消毒剂对小鼠术后近期听性脑干反应的影响%Ototoxicity of Conventional Preoperative Antiseptics

    Institute of Scientific and Technical Information of China (English)

    周韧; 顾晓东; 李健; 王云峰

    2012-01-01

    Objective To evaluate and compare the ototoxicity of chiorhcxidinc, povidonc -iodine, alcohol, and bcnzalkonium bromide tincture-four conventional antiseptics used before the car surgery. Methods ICR mice were randomly assigned to five groups, each group receiving individually application of a different antiseptics or salinc: chlorhcxidinc, povidonc-iodine, alcohol, bcnzalkonium bromide tincture, and saline as control. The baseline of all mice's ABR was recorded before experiment. Kach animal received application of the antiseptics or saline into both middle cars and washed away 5 min later. ABR was tested after 24 hours and compared with early baseline measurements. Results Administration of saline didn't affect ABR threshold. In contrast,significant changes were detected in the ABRs thresholds of all the application of antiseptics. Conclusion Prcopcrativc antiseptics had a clear toxic effect on the cochlcar function in ICR mice. Our results strongly suggested that antiseptics should be avoided to flow into middle car through eardrum perforation before the surgery.%目的 观察术前消毒剂醋酸氯己定(安尔碘)、聚维酮碘(点而康)、乙醇、苯扎溴铵酊对听功能的影响.方法 25只ABR反应阈正常的ICR小鼠随机分为5组,每组5只.将醋酸氯己定、聚维酮碘、乙醇、苯扎溴铵酊及生理盐水分别注入各组双耳中耳腔内,5 min后吸出消毒剂,24 h后观察小鼠8、16、24、32 kHz短纯音ABR反应阈变化.结果 醋酸氯己定、聚维酮碘、乙醇、及苯扎溴铵酊组小鼠8、16、24、36 kHz ABR反应阈均较实验前明显升高(均为P<0.05),而生理盐水对照组实验前后ABR反应阈无显著变化.结论 皮肤消毒剂进入中耳可导致小鼠听力下降,因此,术前消毒时应避免消毒剂通过外耳道及穿孔的鼓膜进入中耳.

  16. Class 1 Integrons and the Antiseptic Resistance Gene (qacEΔ1) in Municipal and Swine Slaughterhouse Wastewater Treatment Plants and Wastewater—Associated Methicillin-Resistant Staphylococcus aureus

    OpenAIRE

    Min Tao Wan; Chin Cheng Chou

    2015-01-01

    Class 1 integrons are mobile gene elements (MGEs) containing qacEΔ1 that are resistant to quaternary ammonium compound (QAC) disinfectants. This study compared the abundances of class 1 integrons and antiseptic resistance genes in municipal (M) and swine slaughterhouse (S) wastewater treatment plants (WWTPs) and investigated the presence of class 1 integrons and antiseptic resistance genes in methicillin-resistant Staphylococcus aureus (MRSA) isolated from wastewater samples. The abundances o...

  17. In vitro investigation of the follicular penetration of porcine ear skin using a nanoparticle-emulsion containing the antiseptic polihexanide In vitro investigation of the follicular penetration of porcine ear skin

    Science.gov (United States)

    Ulmer, M.; Patzelt, A.; Vergou, T.; Lademann, J.; Richter, H.; Kramer, A.; Müller, G.; Sterry, W.; Lange-Asschenfeldt, B.

    2012-05-01

    Earlier investigations regarding the distribution of the bacterial flora on the human skin demonstrate that the hair follicle acts as a bacterial reservoir, providing a quick source for secondary recontamination. These findings highlight the importance of the hair follicle as a target for modern antiseptics. In the present study, we have assessed the follicular penetration of a curcumin-labeled particle-associated antiseptic into porcine skin by laser scanning microscopy. Therefore, the follicular penetration depth of the curcumin-labeled particle-associated antiseptic was compared to the follicular penetration depth of curcumin-labeled particles without antiseptic. The investigation was performed in vitro using porcine skin biopsies. By superposition of the images acquired in the transmission and the fluorescent modus, it was possible to visualize the distribution of the fluorescent dye inside the hair follicles. Quantitative and qualitative results showed that both dispersions penetrated efficiently into the hair follicles. The average penetration depth of the particles with attached antiseptic polihexanide was significantly higher than that of particles without the attached antiseptic. Also, whilst very little sample preparation was needed, laser scanning microscopy was found to be an efficient tool to visualize the skin relief and in particular the hair follicle shaft and localize fluorescent markers within the skin tissue and hair follicles.

  18. Prospective evaluation of 2% (w/v alcoholic chlorhexidine gluconate as an antiseptic agent for blood donor arm preparation

    Directory of Open Access Journals (Sweden)

    Sweta Shah

    2014-01-01

    Full Text Available Aim: A prospective study was undertaken to evaluate the use of 2% (w/v alcoholic chlorhexidine gluconate (2% AlcCHG in donor arm preparation, to monitor the contamination rate of blood products after the collection and to find incidence of transfusion associated bacteremia. Settings and Design: Optimal skin antisepsis of the phlebotomy site is essential to minimize the risk of contamination. Food and Drug Administration (FDA in India has recommended antisepsis with three-step regimen of spirit-10% povidone iodine-spirit for donor arm antisepsis, but not with chlorhexidine, which is recommended by many other authors. Material and Methods: A total of 795 donors were studied from July 2011 to January 2012. Spirit-10% povidone iodine-spirit was used for 398 donors and 2% AlcCHG was used for 397 donors with the two-step method for arm antisepsis. Swabs were collected before and after use of antiseptic agents for all the donors. All the blood products collected from donors with growth in post-antisepsis swabs were cultured. A total of 123 various blood products were cultured irrespective of the method and result of antisepsis was observed. A total of seven patients had mild transfusion reaction. The transfused blood products, blood and urine specimen of the patients who had transfusion reaction were also cultured. Results: Seven donors out of 398 donors had growth in post-antisepsis swab with spirit-10% povidone iodine-spirit protocol and three donors out of 397 donors had growth in post-antisepsis swab with 2% AlcCHG protocol. All blood products collected from donors who had growth in post-antisepsis swabs when cultured had no growth. There was no contamination of blood products. Conclusions: Two percent (w/v alcoholic chlorhexidine gluconate with two-step protocol can be used as an antiseptic agent for donor arm preparation without considerable cost difference. It is at par with spirit 10% povidone iodine spirit protocol as suggested by FDA in India

  19. Safety assessment for ethanol-based topical antiseptic use by health care workers: Evaluation of developmental toxicity potential.

    Science.gov (United States)

    Maier, Andrew; Ovesen, Jerald L; Allen, Casey L; York, Raymond G; Gadagbui, Bernard K; Kirman, Christopher R; Poet, Torka; Quiñones-Rivera, Antonio

    2015-10-01

    Ethanol-based topical antiseptic hand rubs, commonly referred to as alcohol-based hand sanitizers (ABHS), are routinely used as the standard of care to reduce the presence of viable bacteria on the skin and are an important element of infection control procedures in the healthcare industry. There are no reported indications of safety concerns associated with the use of these products in the workplace. However, the prevalence of such alcohol-based products in healthcare facilities and safety questions raised by the U.S. FDA led us to assess the potential for developmental toxicity under relevant product-use scenarios. Estimates from a physiologically based pharmacokinetic modeling approach suggest that occupational use of alcohol-based topical antiseptics in the healthcare industry can generate low, detectable concentrations of ethanol in blood. This unintended systemic dose probably reflects contributions from both dermal absorption and inhalation of volatilized product. The resulting internal dose is low, even under hypothetical, worst case intensive use assumptions. A significant margin of exposure (MOE) exists compared to demonstrated effect levels for developmental toxicity under worst case use scenarios, and the MOE is even more significant for typical anticipated occupational use patterns. The estimated internal doses of ethanol from topical application of alcohol-based hand sanitizers are also in the range of those associated with consumption of non-alcoholic beverages (i.e., non-alcoholic beer, flavored water, and orange juice), which are considered safe for consumers. Additionally, the estimated internal doses associated with expected exposure scenarios are below or in the range of the expected internal doses associated with the current occupational exposure limit for ethanol set by the Occupational Safety and Health Administration. These results support the conclusion that there is no significant risk of developmental or reproductive toxicity from

  20. Molecular dynamics and docking simulation of a natural variant of Activated Protein C with impaired protease activity: implications for integrin-mediated antiseptic function.

    Science.gov (United States)

    D'Ursi, Pasqualina; Orro, Alessandro; Morra, Giulia; Moscatelli, Marco; Trombetti, Gabriele; Milanesi, Luciano; Rovida, Ermanna

    2015-01-01

    Activated Protein C (APC) is a multifunctional serine protease, primarily known for its anticoagulant function in the coagulation system. Several studies have already elucidated its role in counteracting apoptosis and inflammation in cells, while significant effort is still ongoing for defining its involvement in sepsis. Earlier literature has shown that the antiseptic function of APC is mediated by its binding to leukocyte integrins, which is due to the presence of the integrin binding motif Arg-Gly-Asp at the N-terminus of the APC catalytic chain. Many natural mutants have been identified in patients with Protein C deficiency diagnosis including a variant of specificity pocket (Gly216Asp). In this work, we present a molecular model of the complex of APC with αVβ3 integrin obtained by protein-protein docking approach. A computational analysis of this variant is hereby presented, based on molecular dynamics and docking simulations, aiming at investigating the effects of the Gly216Asp mutation on the protein conformation and inferring its functional implications. Our study shows that such mutation is likely to impair the protease activity while preserving the overall protein fold. Moreover, superposition of the integrin binding motifs in wild-type and mutant forms suggests that the interaction with integrin can still occur and thus the mutant is likely to retain its antiseptic function related to the neutrophyl integrin binding. Therapeutic applications could result in this APC mutant which retains antiseptic function without anticoagulant side effects.

  1. Antagonistic Interactions of “Ya-Sa-Marn-Phlae” Ethanol Extract in Combination with Topical Antiseptics against Clinical Isolates of Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Sasitorn Chusri

    2014-01-01

    Full Text Available This investigation was aimed at assessing a possible interaction of a traditional Thai herbal recipe, “Ya-Sa-Marn-Phlae (YSMP,” used for wound treatments with topical antiseptics, povidone-iodine (PI solution and hydrogen peroxide (H2O2, and effects of THR-SK010 alone and the combinations on Staphylococcus aureus. Antibacterial activities of ethanol extracts from the herbal recipe were determined against both methicillin resistant Staphylococcus aureus (MRSA and methicillin-susceptible S. aureus (MSSA. YSMP exhibited remarkable antistaphylococcal activity with MIC values of 3.9–7.8 μg/mL. This recipe possessed bacteriostatic activity and did not reduce the tolerance of both MRSA and MSSA isolates to the high ionic strength. Interaction between THR-SK010 and the antiseptics was carried out by checkerboard testing and time-kill assay. Both indifferent and slightly antagonistic effects were observed with THR-SK010/PI and THR-SK010/H2O2 combinations against the tested isolates. In addition to commercially available antiseptics, THR-SK010 offered additional therapeutic options for the decolonization of MRSA and MSSA. Topical application of plant extracts with antioxidant activity, such as THR-SK010, should not be used immediately with PI or H2O2 and further investigation on this interaction is needed.

  2. 台湾香肠制作中复合防腐剂的研究%Research on Compound Antiseptic Applying to the Taiwan Sausage

    Institute of Scientific and Technical Information of China (English)

    韩毅; 杨振泉; 方维明

    2011-01-01

    The paper studied the function of several kinds of antiseptics on enhancing the shelf time of Taiwan Sausage product,and the best antiseptic combination was screened through the single factors experiment and orthogonal experiments.Fresh keeping effect was assessed by the total number of bacteria,TVB-N and pH value,and the results indicated that the best fresh keeping effect was obtained when using combination of four antiseptics.%研究添加不同防腐剂对低温灌肠制品(台湾香肠)保质期的影响。设计单因素和正交试验,分别将不同浓度防腐剂及其组合在台湾香肠制作工序中(二次搅拌)加入原料内,分别对成品台湾香肠细菌总数、TVB-N值、pH值的变化进行测定,筛选最佳的防腐剂组合。结果表明:4种防腐剂复合后使用效果最佳。

  3. Comparison of in vivo antiseptic and in vitro antimicrobial effects of Peganum harmala L. seeds ethanolic extract with Betadine

    Institute of Scientific and Technical Information of China (English)

    Ali Asghar Khademalhosseini; Abutorab Tabatabaei; Paria Akbari; Mohammad Saeed Fereidouni; Mostafa Akhlaghi

    2015-01-01

    Objective: To compare the antibacterial activity and antiseptic effect of ethanol extract of Peganum harmala L. (P. harmala) seeds and Betadine on eight reference strains of Streptococcus pneumoniae, Salmonella typhimurium, Escherichia coli, Corynebacterium pseudotuberculosis, Klebsiella pneumoniae, Staphylococcus aureus, Micrococcus luteus which are known to cause different types of skin infections, and Corynebacterium pseudotuberculosis isolated from an abscess on horse's neck. Methods:The antibacterial activity was assessed using a disc diffusion method. The minimum inhibitory concentration was tested by serial dilution method and the percentage of bacterial growth inhibition by absorbance microplate reader device. The minimum bactericidal concentration was then recorded. Clinical outcomes were obtained through washed up healing time of longitudinal and surface skin on the back of 16 rats with concentrations of 10, 50, 100, 150 and 500 mg/mL of P. harmala extract. Results: The results of in vitro experiments showed that the lowest minimum inhibitory concentration (0.68-1.3 mg/mL) and minimum bactericidal concentration (1.3-5 mg/mL) values were observed in the ethanol extract of P. harmala seeds. Also the results of in vivo experiment showed that wound healing in the concentration of 50 mg/mL of this plant extract was better and quicker than Betadine. Conclusions:This study confirmed that ethanol extract of P. harmala has appropriate effect on the microorganisms and the healing of skin wounds in comparison with Betadine.

  4. Comparison of in vivo antiseptic and in vitro antimicrobial effects of Peganum harmala L. seeds ethanolic extract with Betadine

    Directory of Open Access Journals (Sweden)

    Ali Asghar Khademalhosseini

    2015-01-01

    Full Text Available Objective: To compare the antibacterial activity and antiseptic effect of ethanol extract of Peganum harmala L. (P. harmala seeds and Betadine on eight reference strains of Streptococcus pneumoniae, Salmonella typhimurium, Escherichia coli, Corynebacterium pseudotuberculosis, Klebsiella pneumoniae, Staphylococcus aureus, Micrococcus luteus which are known to cause different types of skin infections, and Corynebacterium pseudotuberculosis isolated from an abscess on horse's neck. Methods: The antibacterial activity was assessed using a disc diffusion method. The minimum inhibitory concentration was tested by serial dilution method and the percentage of bacterial growth inhibition by absorbance microplate reader device. The minimum bactericidal concentration was then recorded. Clinical outcomes were obtained through washed up healing time of longitudinal and surface skin on the back of 16 rats with concentrations of 10, 50, 100, 150 and 500 mg/mL of P. harmala extract. Results: The results of in vitro experiments showed that the lowest minimum inhibitory concentration (0.68-1.3 mg/mL and minimum bactericidal concentration (1.3-5 mg/mL values were observed in the ethanol extract of P. harmala seeds. Also the results of in vivo experiment showed that wound healing in the concentration of 50 mg/mL of this plant extract was better and quicker than Betadine. Conclusions: This study confirmed that ethanol extract of P. harmala has appropriate effect on the microorganisms and the healing of skin wounds in comparison with Betadine.

  5. Antimicrobial Activity of Lippia Species from the Brazilian Semiarid Region Traditionally Used as Antiseptic and Anti-Infective Agents

    Directory of Open Access Journals (Sweden)

    Cristiana da Purificação Pinto

    2013-01-01

    Full Text Available Lippia origanoides Kunth, Lippia alnifolia Schauer, and Lippia thymoides Martius and Schauer are shrubs used in the traditional Brazilian medicine as antiseptics, as well as in the treatment of infectious diseases. This study was designed to investigate the antibacterial and antifungal activities of the methanolic extracts of these species, as new potential sources of antimicrobial drugs. The antimicrobial activity of methanolic extracts was investigated against resistant yeasts and bacteria by agar disk diffusion. Then, the MIC determination of the most active species and its fractions in hexane, dichloromethane, ethyl acetate, and water was performed. By the agar diffusion assay, all species were active against at least two microorganisms, giving evidence to support their use in the popular medicine. L. origanoides leaves exhibited the widest antimicrobial action, inhibiting the growth of two Gram-positive bacteria and two yeasts; this activity was also confirmed by the MIC evaluation. The fractionation of L. origanoides crude extracts improved the activity in spectrum and intensity. The results obtained in this study indicate that L. origanoides may be a promising alternative in the treatment of bacterial and fungal infections and in the seeking of new antimicrobial drugs.

  6. 一种免冲洗手消毒乳液杀菌效果的观察%OBSERVATION ON GERMICIDAL EFFECT OF A HAND ANTISEPTIC AGENT EMULSION

    Institute of Scientific and Technical Information of China (English)

    钱蕊硃; 银燕; 贾兴真

    2012-01-01

    To observe the germicidal effect of a hand antiseptic agent emulsion. Methods Suspension quantitative germicidal test and field disinfectant test of hand and skin are used to observe germicidal effect of the hand antiseptic agent. Results The main germicidal ingredients of this compound hand antiseptic agent emulsion are 5. 66 g/L chlorhexidine gluconate and 40% ethanol. the killing logarithm value of Staphylococeus aureus and Escherichia coli for 1 min, of Pseudomonas aeruginosa for 5 min are all >5. 00. The killing logarithm value of Candida albicans for 1 min is >4.00. Wipe the hands of subjects with the antiseptic agent for 1. 0 min, the killing logarithm value of natural germ on hands is > 1.00, the killing logarithm value of skin germ is > 1.00. Conclusion The germicidal effect of this hand antiseptic agent is good with stable property.%目的 观察一种免冲洗手消毒乳液的杀菌效果.方法 采用悬液定量杀菌试验和现场消毒试验方法,对该手消毒乳液进行了试验观察.结果 该手消毒乳液原液是由含5.66 g/L葡萄糖酸氯己定与体积分数40%乙醇等组成的复方手消毒液.以其原液作用1.0 min,对悬液内金黄色葡萄球菌和大肠杆菌平均杀灭对数值>5.00;对悬液内铜绿假单胞菌作用5 min,平均杀灭对数值>5.00;对悬液内白色念珠菌作用1 min,平均杀灭对数值>4.00.用该手消毒乳液原液对受试者手擦拭消毒1 min,对手部自然菌的平均杀灭对数值>1.00;用该手消毒乳液原液对受试者皮肤擦拭消毒5 min,对皮肤自然菌的平均杀灭对数值>1.00.结论 该复方手消毒乳液杀菌效果良好,性能稳定.

  7. Novel antiseptic urinary catheters for prevention of urinary tract infections: correlation of in vivo and in vitro test results.

    Science.gov (United States)

    Hachem, Ray; Reitzel, Ruth; Borne, Agatha; Jiang, Ying; Tinkey, Peggy; Uthamanthil, Rajesh; Chandra, Jyotsna; Ghannoum, Mahmoud; Raad, Issam

    2009-12-01

    Urinary catheters are widely used for hospitalized patients and are often associated with high rates of urinary tract infection. We evaluated in vitro the antiadherence activity of a novel antiseptic Gendine-coated urinary catheter against several multidrug-resistant bacteria. Gendine-coated urinary catheters were compared to silver hydrogel-coated Foley catheters and uncoated catheters. Bacterial biofilm formation was assessed by quantitative culture and scanning electron microscopy. These data were further correlated to an in vivo rabbit model. We challenged 31 rabbits daily for 4 days by inoculating the urethral meatus with 1.0 x 10(9) CFU streptomycin-resistant Escherichia coli per day. In vitro, Gendine-coated urinary catheters reduced the CFU of all organisms tested for biofilm adherence compared with uncoated and silver hydrogel-coated catheters (P < 0.004). Scanning electron microscopy analysis showed that a thick biofilm overlaid the control catheter and the silver hydrogel-coated catheters but not the Gendine-coated urinary catheter. Similar results were found with the rabbit model. Bacteriuria was present in 60% of rabbits with uncoated catheters and 71% of those with silver hydrogel-coated catheters (P < 0.01) but not in those with Gendine-coated urinary catheters. No rabbits with Gendine-coated urinary catheters had invasive bladder infections. Histopathologic assessment revealed no differences in toxicity or staining. Gendine-coated urinary catheters were more efficacious in preventing catheter-associated colonization and urinary tract infections than were silver hydrogel-coated Foley catheters and uncoated catheters.

  8. The study for antiseptic activity of Pumpkin%南瓜不同部位抗菌活性研究

    Institute of Scientific and Technical Information of China (English)

    向阳; 董昌金

    2014-01-01

    利用乙醇、甲醇和蒸馏水作提取剂分别提取南瓜不同部位(瓜皮、瓜肉、瓜瓤)中的抗菌物质,并通过滤纸片法、琼脂孔扩散法测定其抗菌活性。实验结果显示,南瓜的甲醇提取液对枯草芽孢杆菌(G+)和大肠杆菌(G-)的抑菌作用更为明显,且瓜瓤中抗菌物质含量最为丰富;南瓜的甲醇-瓤提取液对大肠杆菌的最低抑菌浓度为10%,对枯草芽孢杆菌的最低抑菌浓度为15%;初步鉴定瓜瓤中有良好抗菌活性的成分为脂溶性物质。%In this study, the antibacterial material was extracted by 95%ethyl alcohol, methyl alcohol and distilled water in different parts of the ripe Pumpkin(pericarp, sarcocarp and melon pulp).Filter paper method and agar diffusion method were used to measure the antiseptic activity of antibacterial material .The trial results showed that inhibitory effect of the methyl al-cohol extract of pumpkin on Bacillus subtilis ( G+) and Escherichia coli ( G-) was more obvious than others , and the anti-bacterial material content is most abundant in the melon pulp .The minimum inhibition concentration ( MIC) of the antibacteri-al material to E.coli was 10%, and 15%to B.subtilis.Preliminary conclusion can be drawn that antibacterial activity com-ponents were liposoluble substance in the melon pulp .

  9. The Synthesis of Antiseptic Sodium Diacetate%新型防霉剂双乙酸钠的合成工艺初报

    Institute of Scientific and Technical Information of China (English)

    徐清海; 李秉超

    2000-01-01

    以乙酸酐和氢氧化钠为原料合成了新型防霉剂双乙酸钠,其生产工艺简单、操作方便、收率高,产品符合质量标准要求。%Sodium diacetate as a new type antiseptic was synthetized using acetic anhydride and sodium hydroxide. The technique of manufacture was simple and the method of operation was easy, the yield was high. The quality of the procduct accorded with the FAO/WHO standard.

  10. Antimicrobial Analysis of an Antiseptic Made from Ethanol Crude Extracts of P. granatum and E. uniflora in Wistar Rats against Staphylococcus aureus and Staphylococcus epidermidis

    Directory of Open Access Journals (Sweden)

    Thaís Honório Lins Bernardo

    2015-01-01

    Full Text Available Introduction. Surgical site infection remains a challenge for hospital infection control, especially when it relates to skin antisepsis in the surgical site. Objective. To analyze the antimicrobial activity in vivo of an antiseptic from ethanol crude extracts of P. granatum and E. uniflora against Gram-positive and Gram-negative bacteria. Methods. Agar drilling and minimal inhibitory tests were conducted for in vitro evaluation. In the in vivo bioassay were used Wistar rats and Staphylococcus aureus (ATCC 25923 and Staphylococcus epidermidis (ATCC 14990. Statistical analysis was performed through variance analysis and Scott-Knott cluster test at 5% probability and significance level. Results. In the in vitro, ethanolic extracts of Punica granatum and Eugenia uniflora and their combination showed the best antimicrobial potential against S. epidermidis and S. aureus. In the in vivo bioassay against S. epidermidis, there was no statistically significant difference between the tested product and the patterns used after five minutes of applying the product. Conclusion. The results indicate that the originated product is an antiseptic alternative source against S. epidermidis compared to chlorhexidine gluconate. It is suggested that further researches are to be conducted in different concentrations of the test product, evaluating its effectiveness and operational costs.

  11. Selection of Antiseptic Agents for 50%Magnesium Sulfate Solution%50%硫酸镁溶液的防腐剂筛选研究

    Institute of Scientific and Technical Information of China (English)

    袁惠英

    2011-01-01

    OBJECTIVE: To select the type and concentration of antiseptic agents for 50% magnesium sulfate solution, and to improve the quality of magnesium sulfate solution.METHODS: 50% magnesium sulfate solution were added 0.01% ethylparaben,0.02% ethylparaben, 0.03% benzoic acid, 0.05% benzoic acid, 0.01% domiphen bromide, 0.02% domiphen bromide, 0.03% hydrogen peroxide, 0.06% hydrogen peroxide, 0.01% benzalkonium bromide, and 0.02% benzalkonium bromide, referring to commonly used inhibitory concentration of antiseptic agents.The dissolution of antiseptic agents was observed under normal temperature or cool condition.The content determination and microbial limit test of magnesium sulfate were conducted at different time (within 12 months).RESULTS: Except 0.01% ethylparaben bromide, 0.02% ethylparaben bromide and 0.05% benzoic acid, other antiseptic agents dissolved completely in 50% magnesium sulfate solution.The content determination and microbial limit test of magnesium sulfate solution added hydrogen peroxide, benzalkonium bromide, domiphen bromide, and benzoic acid after 12 months were in line with the standard of content determination and microbial limit test.CONCLUSION: 0.03% hydrogen peroxide, 0.01% benzalkonium bromide, 0.01% domiphen bromide or 0.03% benzoic acid could be selected as antiseptic agents for 50% magnesiun sulfate solution.%目的:筛选50%硫酸镁溶液的防腐剂的种类及浓度,以提高硫酸镁溶液的质量.方法:参照防腐荆的常用抑菌浓度,在50%硫酸镁溶液中分别加入0.01%、0.02%的羟苯乙酯,0.03%、0.05%的苯甲酸,0.01%、0.02%的度米芬,0.03%、0.06%的过氧化氢和0.01%、0.02%的苯扎溴铵,置于常温或冷处条件下观察其溶解情况,并在不同留样期(12个月内)对硫酸镁进行含量测定及微生物限度检查.结果:除0.01%、0.02%的羟苯乙酯和0.05%的苯甲酸外,其余浓度防腐剂均在50%硫酸镁溶液中溶解完全;含有过氧化

  12. MODIFICATION OF CELLULOSE MATERIALS BY ANTISEPTICS AND THEIR ANTIMICROBIAL PROPERTIES(Ⅱ)--Release of antiseptics from modified cellulose materials and their antimicrobial activity%抗菌剂改性纤维素物料及其抗菌性能(Ⅱ)--改性纤维素物料抗菌素的释出及其抗菌性能

    Institute of Scientific and Technical Information of China (English)

    柯捷里尼科娃N E; 侯永发; 帕纳林E F; 库吉娜N P; 李淑秀; 扎伊金娜N A

    2000-01-01

    抗菌剂(CA)改性棉纱布(CM)和漂白棉布(CC)系经CA与CM和CC相互吸附作用而制成.本文研究了在不同pH值溶液中抗菌素释出状况.试验结果证明,在pH=7.0时抗菌素释出量最大.改性纤维素物料的微生物试验表明其具有抗菌性能,作为重要医疗包扎物品,具有实际应用价值.%Dressing cellulose materials(CM) and bleached cotton cloth (CC) modified by surfactant antiseptics(CA) have been prepared by the method of adsorption interaction between CM and CA. Antiseptics release was studied by the method of desorption in solutions at different pH values. Maximum release of surfactants is achieved in solutions at pH=7.0. Microbiological tests of modified cellulose materials have shown that they exhibit antimicrobial activity. These results are valuable for practical application in clinics for imparting antimicrobial properties to dressing materials.

  13. OBSERVATION ON THE in vitro DISINFECTION EFFECT OF A RAPID HAND ANTISEPTIC%一种快速手消毒剂现场消毒效果观察

    Institute of Scientific and Technical Information of China (English)

    李荷花; 王苗凤; 马玲平

    2013-01-01

    提要 目的 观察一种快速手消毒剂的实际消毒效果.方法 通过现场消毒试验方法,对该快速手消毒剂对外科手实际消毒效果进行了观察,同时与聚维酮碘作平行比较.结果 两组各200人次分别使用快速手消毒剂和聚维酮碘消毒后,即刻、60、120、180 min等4个时段采样检测,全部无细菌生长.200人次使用某品牌快速手消毒剂消毒手部皮肤,无1例出现红斑、水肿和皮疹,有3例出现瘙痒,不良反应率为1.5%.200人次使用聚维酮碘消毒手部皮肤,不良反应率为6%.结论 快速手消毒剂和聚维酮碘对医务人员外科手消毒效果无差别,消毒后均未培养出存活细菌,但快速手消毒剂消毒后皮肤速干性好,不良反应率低.%Objective To observe the actual disinfection effect of a rapid hand antiseptic. Methods The in vitro disinfection test was used to compare the actual disinfection effect of this rapid hand antiseptic with povidone iodine. Results There was no bacteria detected in samples of four times(immediate,60min,120min ,180min) from two groups whose hands exposed to the rapid hand antiseptic and povidone iodine respectively. There was no case of skin erythema or dropsy and rash while 3 cases of itching were found in the experiment group including 200 people exposed to this rapid hand disinfection antiseptic. The adverse reaction rate was 1. 5% (3/200) in experiment group and that was 6% in control group( povidone iodine group). Conclusion The disinfection effects of a rapid hand antiseptic and povidone iodine on hands of medical staff have no difference and there is no bacteria after disinfection. But there are quick dry skin and low adverse reaction rate after disinfection with a rapid hand antiseptic.

  14. 民政专用纳米光催化空气净化消毒机的设计与实现%Design and implementation of civil administration dedicated nano-TiO2 photocatalytic air antiseptic equipment

    Institute of Scientific and Technical Information of China (English)

    李秉杰; 王志良; 李伯森; 张培昆; 刘冀伟; 孟浩

    2013-01-01

    In order to reduce the air pollution discharged by funeral parlor of civil administration section during the funeral processes ,special antiseptic equipment was developed to purify the in-door air .Based on the filter technology ,activated carbon adsorption technology and photo-cata-lytic technology ,an intellectualized air flow control system was designed applying the modified chaos algorithm and PID optimal control algorithm .Then the prototype equipment was built at the optimal parameters .As shown by the experimental results ,the designed nano-TiO2 photo-catalytic antiseptic equipment is easy to operate and has a powerful antiseptic ability .This equip-ment can satisfyingly disinfect the air ,and therefore can replace the ultraviolet antiseptic equip-ment in the funeral parlor .%  为解决民政行业殡仪场所空气污染问题,研制了一种专用于殡仪场所空气净化的消毒机。以滤网过滤技术、活性炭吸附技术、纳米光催化技术为设计基础,应用改进混沌优化算法与智能PID 最优控制算法设计了智能风面控制系统,优化设计了空气消毒机的结构参数,最后制作样机。结果表明,设计的纳米TiO2光催化空气净化消毒机具有良好的消毒能力且操作简便,对环境空气具有良好的消毒净化功能,可替代紫外线用于殡仪场所空气净化和消毒。

  15. 抗感染中心静脉导管预防导管相关性血流感染的效果%Effect in prevention of catheter-related bloodstream infection using antiseptic impregnated central venous catheter

    Institute of Scientific and Technical Information of China (English)

    罗蕾; 秦英; 向亚娟

    2011-01-01

    Objective To evaluate the clinical effect in prevention of catheter-related bloodstream infection(CRBSI) using antiseptic impregnated central venous catheter. Methods The incidence of catheterrelated bloodstream infection was compared between 420 cases performed with antiseptic impregnated central venous catheter ( experimental group ) and 430 cases performed with general central venous catheter (control).Besides, the categories and characteristics of pathogenic bacteria which resulted in catheter-related bloodstream infection were analyzed. Results There were 66 cases of CRBSI in 850 cases, 25 cases were inserted antiseptic impregnated central venous catheter and 41 cases inserted general central venous catheter. No significant difference in the incidence between the two groups was found during 7 days(χ2 = 0. 06 ,P > 0. 05), however, the incidence of CRBSI was lower in the experimental group than of the control group after 7 days( χ2 = 3.91,4.30 ;P 0.05),7 d后实验组CRBSI感染率较对照组低,差异有统计学意义(χ2分别为3.91,4.30,P<0.05).实验组减少导管相关性血流感染的病原体主要为革兰阳性菌.结论 抗感染中心静脉导管可明显减少导管相关性血流感染的发生,有很大的临床价值.

  16. In Vitro bactericidal experiment of an'erdian skin antiseptic on common drug resistant bacteria%安尔碘皮肤消毒剂对常见耐药细菌的体外杀菌试验

    Institute of Scientific and Technical Information of China (English)

    唐毅; 萧晨路; 李和姐; 倪语星

    2011-01-01

    目的 了解安尔碘皮肤消毒剂对临床耐药菌的最小杀菌浓度.方法 采用对倍稀释法,以安尔碘皮肤消毒剂不同浓度对耐甲氧西林金黄色葡萄球菌(MRSA)和泛耐药鲍氏不动杆菌作用不同时间,观察杀菌效果.结果 安尔碘皮肤消毒剂在有效碘含量为8.125μg/ml时,作用1 min后,可将检测的所有菌株全部杀灭.结论 安尔碘皮肤消毒剂为强而有效的杀菌剂.%OBJECTIVE To understand the minimum bactericidal concentration of An'erdian Skin Antiseptic to drug resistant strains in clinics.METHODS Different concentrations of An'erdian Skin Antiseptic were applied to MRSA and pan-drug resistant Acinetobacter baumannii suspension for periods of time, then the sterilization effects were observed.RESULTS All of the drug resistant strains were eradicated with the effective content of iodine was 8.125 μg/ml and was applied for 1 min.CONCLUSION An' erdian skin antiseptic is a kind of strong and effective disinfectant.

  17. Synthesis of a new molecularly imprinted polymer for sorption of the silver ions from geological and antiseptic samples for determination by flame atomic absorption spectrometry.

    Science.gov (United States)

    Hashemi-Moghaddam, Hamid; Yahyazadeh, Faegheh; Vardini, Mohammad Taghi

    2014-01-01

    A new molecularly imprinted polymer (MIP) was synthesized using methacrylic acid (functional monomer), ethylene glycol dimethacrylate (crosslinker), 2,2'-azobisisobutironitril (initiator), silver (Ag) dithizone complex (template), and chloroform (porogenic solvent). This process was a noncovalent, bulk, thermal radical-polymerization. To compare the performance of this polymer, control polymer (nonimprinted polymer) was prepared under well-defined conditions without the use of a template. Extraction experiments were performed on the MIP and a nonimprinted polymer. Then, various parameters were optimized, such as pH, time, concentration of sample, and type of eluent for elution of Ag from polymer. In addition, interfering effects were investigated on the absorption of Ag by the MIP. This polymer was used for the rapid extraction and preconcentration of Ag from an antiseptic and geological sample. Finally, the amount of Ag was measured by flame atomic absorption spectrometry after preconcentration by the synthesized MIP, and results were compared with a direct inductively coupled plasma method. The results showed high performance of this method in preconcentration of Ag. PMID:25902996

  18. Carbohydrate derived fulvic acid (CHD-FA: an in vitro investigation of a novel membrane active antiseptic agent against Candida albicans biofilms

    Directory of Open Access Journals (Sweden)

    Leighann eSherry

    2012-03-01

    Full Text Available Carbohydrate derived fulvic acid (CHD-FA is a heat stable low molecular weight, water soluble, cationic, colloidal material with proposed therapeutic properties. The aim of this study was to evaluate the antifungal activity of CHD-FA against Candida albicans, and to characterise its mode of action. A panel of C. albicans isolates (n=50 derived from a range of clinical specimens were grown planktonically and as biofilms, and the minimum inhibitory concentrations (MICs determined. Scanning electron microscopy was performed to examine ultrastructural changes and different cell membrane assays were used to determine its mode of action. In addition, the role of C. albicans biofilm resistance mechanisms were investigated to determine their effects of CHD-FA activity. CHD-FA was active against planktonic and sessile C. albicans at concentrations 0.125% and 0.25% respectively, and was shown to be fungicidal, acting through disruption of the cell membrane activity. Resistance mechanisms, including matrix, efflux and stress, had a limited role upon CHD-FA activity. Overall, based on the promising in vitro spectrum of activity and minimal biofilm resistance of the natural and cheap antiseptic CHD-FA, further studies are required to determine its applicability for clinical use.

  19. Antisépticos orales: Revisión de la literatura y perspectiva actual Oral antiseptics: Review of the literature and actual perspectives

    Directory of Open Access Journals (Sweden)

    A Bascones

    2006-04-01

    Full Text Available El control de placa bacteriana es el método principal en la prevención de las enfermedades periodontales. Cada vez está más extendido el denominado control químico de la placa de manera complementaria a un control mecánico ineficaz. Los fármacos más utilizados a tal fin son los antisépticos bucodentales, siendo ampliamente aceptada la clorhexidina como el de mayor eficacia. Existen sin embargo numerosas formulaciones de distintos principios activos, ante lo cual, parece justificado revisar la literatura con el objetivo de aclarar la eficacia de los mismos, así como sus diferentes indicaciones.The plaque control is the main method in the prevention of periodontal diseases. It´s well known the use of chemical plaque control as a complement to unadequate mechanical control. The most used drugs have been the oral antiseptics, being the chlorhexidine the gold standar. There are many formulations with different chemical components; therefore it seems to be useful to review their properties, efficacy and indications.

  20. Prevalence of antiseptic resistance genes increases in staphylococcal isolates from orthokeratology lens wearers over initial six-month period of use.

    Science.gov (United States)

    Guang-Sen, Shi; Boost, Maureen; Cho, Pauline

    2016-06-01

    The purpose of this study was to investigate the prevalence of antiseptic-resistance (QAC) genes in staphylococci colonizing periorbital tissues and accessories of orthokeratology (ortho-k) lens wearers over a 6-month period and determine the effect of their presence on minimum inhibitory (MIC) and bactericidal concentrations (MBC) of disinfectants and log reduction of multipurpose contact lens solutions (MPS). Staphylococci were isolated from periorbital tissues and accessories of patients before commencing ortho-k therapy and at two subsequent visits. Presence of QAC genes in 116 S. aureus and 67 CNS isolates was determined by PCR and association with period of ortho-k use determined. MICs and MBCs of staphylococci gene-positive were compared with gene-negative strains and the effectiveness of four MPS for rigid contact lenses investigated. S. aureus carriage rates in the conjunctiva decreased significantly from 41.2 % (baseline) to 11.8 % (3-months), and 13.3 % (6-months) (p trend 0.03), while CNS increased from 58.8 %(baseline) to 94.1 % (3-months), and 93.3 % (6-months) (p trend 0.02). Prevalence of qacA/B increased considerably over time (S. aureus: 4.4 % to 15.4 %, CNS: 6.7 % to 25 %), but frequency of smr was relatively stable. Only five CNS isolates harboured qacH. MICs and MBCs of gene-positive isolates were significantly increased and three MPS did not achieve a 3-log reduction of many QAC-positive strains. Ortho-k lens wear contributed to changes of staphylococcal carriage rates in the conjunctival sac. Use of MPS containing quaternary ammonium compounds may select for the carriage of organisms harbouring QAC genes, as the low concentration of disinfecting agents is not adequate for killing gene-positive strains. PMID:26993290

  1. EXPERIMENTAL STUDY ON DISINFECTION PERFORMANCE OF A KIND OF QUICKDRYING ANTISEPTIC SOLUTION%一种速干性消毒液消毒相关性能实验研究

    Institute of Scientific and Technical Information of China (English)

    苏裕心; 蒋莉; 武雪冰; 任哲; 帖金凤; 张文福

    2012-01-01

    目的 研究一种速干性消毒液消毒相关性能及其影响因素.方法 采用悬液定量杀菌试验和动物试验方法,对该消毒液消毒相关性能进行了实验室观察.结果 该速干性消毒液为一种复方制剂,主要有效成分为8 g/L醋酸氯已定与体积分数70%乙醇.用该消毒液10倍稀释液作用2 min,对悬液内金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌的杀灭率均达到100%.菌悬液内加入有机干扰物对该消毒液杀菌作用有轻度影响,消毒液pH值7.0以上有利于杀菌效果的提高,温度越高杀菌效果越好.该消毒液急性经口毒性LD50值>5 000 mg/kg(体重),其原液对家兔完整皮肤刺激性试验最高积分为0,小鼠骨髓嗜多染红细胞微核试验结果为阴性.结论 该速干性消毒液杀菌作用快速,效果较好;属于基本无毒物质,对完整皮肤无刺激性,无致微核作用.%Objective To study the disinfection performance and influential factors of a kind of quick - drying liquid skin antiseptic with chlorhexidine acetate as the active principle. Methods Suspension quantitative germicidal test and animal toxicity test were used to carry out laboratory observation of this antiseptic. Results At room temperature, the killing rate of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans exposed to the 10 - fold dilu-tion of compound chlorhexidine alcohol antiseptic solution containing chlorhexidine acetate 8 mg/L and 70% (v/v) ethanol for 2 min were 100%. Adding BSA to the bacterial suspension slightly influenced the germicidal efficacy of the antiseptic solution in killing E. Coli . With the increase of pH and temperature, the germicidal efficacy of the 8 - fold dilution of the antiseptic solution could obviously be enhanced in killing E. Coli . The acute oral toxicity test of the disinfection solution showed that LD50 values in both rats were >5 000 mg/kg · bw, which were

  2. 含抗菌成分香皂除菌效果评价方法的研究%Study on evaluating method for bacteria - eliminating efficacy of soap containing antiseptic ingredients

    Institute of Scientific and Technical Information of China (English)

    张伟; 马玲; 李炎; 赵斌秀; 王妍彦; 班海群; 李新武

    2011-01-01

    目的:为含抗菌成分洗手产品的除菌效果评价方法提供依据.方法:对某品牌含抗菌成分的香皂进行了金黄色葡萄球菌和大肠杆菌的除菌效果检测.结果:该香皂对金黄色葡萄球菌和大肠杆菌的细菌减少对数值均大于3.00;受试者的左、右手之间、不同性别的受试者之间,对金黄色葡萄球菌和大肠杆菌的细菌减少对数值均无显著性差异.结论:该香皂具有除菌作用,该方法有很好的重复性和可操作性.%Objective: In order to provide the basis for evaluating method for bacteria - eliminating efficacy of hand - washing products containing antiseptic ingredients.Methods: Examination of bacteria - eliminating efficacy of the soap, which containing antiseptic ingredients, in eliminating Staphylococcus aureus and Escherichia coli.Results: Both of logarithms of Staphylococcus aureus and Escherichia coli count decreased are more than 3.00; The decrease in logarithm of Staphylococcus aureus and Escherichia coli counts on left and right hands of subjects, male and female subjects showed no significant difference.Conclusion: This soap has antiseptic function; this evaluation method is simple and accurate with a good reproducibility and convenient operation.

  3. Study on Antiseptic Property of Water Soluble Polyvinyl Alcohol Building Adhesive%水溶性聚乙烯醇建筑胶粘剂防腐性能研究

    Institute of Scientific and Technical Information of China (English)

    董黎明; 宋明; 周俊; 黄菊

    2016-01-01

    随着游离甲醛含量的降低,水溶性聚乙烯醇建筑胶粘剂长期储存时发霉变质问题越来越引发人们的关注。本研究从生产工艺路线出发研究了胶粘剂发霉变质的原因,采用长期模拟验证实验和微生物侵染实验,研究了CuSO4、NaNO2、A26、CP19和CN46五种防腐剂对胶粘剂的防腐效果。结果表明,不同品种的防腐剂对胶粘剂的防腐性效果不同,CuSO4、NaNO2防腐性能较弱,并会出现离子络合沉淀;含有异噻唑啉酮衍生物类杀菌剂A26、CP19与CN46防腐剂杀菌抑菌效果明显,随着有效物质含量的增加,其抑菌效果越好。研究发现,CN46用量为0.25%时具有最优的长期防腐效果。%With people’s strengthened environmental awareness and the decrease of free formaldehyde content in water soluble polyvinyl alcohol (PVA) building adhesive, the problems about mildewing and change of quality in PVA adhesive are getting people's attention. The reasons for the adhesive mildewing were studied on the aspect of PVA adhesive production process. The different dosage of preservatives, such as CuSO4, NaNO2, A26, CP19 and CN46 which affect on antiseptic properties of adhesive were studied by long-term simulation veriifed experiment and microorganism infection experiment. The results showed that the antiseptic properties of PVA adhesives were affected by different species and dosage of preservatives. The isothiazolone derivatives such as A26, CP19 and CN46 had better antiseptic properties and compatibility than CuSO4 and NaNO2. Research shows that CN46 has optimal and long-term antiseptic effect when its amount was 0.25%.

  4. Analysis of the drug-consumption for oral antiseptic ulcer drugs in our hospital from 2011 to 2013%2011-2013年我院口服抗消化性溃疡药物的用药分析

    Institute of Scientific and Technical Information of China (English)

    吴骏; 陈集志; 徐小燕; 徐江红

    2014-01-01

    Objective To evaluate the medication state and tendency of oral antiseptic ulcer drugs in our hospital in order to ensure clinical medicine supply and management.Methods The sum,pharmacology category,ordering,daily defined doses ( DDDs) and defined daily cost( DDC) of oral antiseptic ulcer drugs in 94 hospital of PLA were analysed statistically.Results The sales amount of oral antiseptic ulcer drugs was increased year by year.The sales amount of proton pump inhibitor ranked first for three years and the top two were lansoprazole capsules and omeprazole capsules in respect of DDDs.Conclusion The medication state of oral antiseptic ulcer drugs in our hospital showed relatively smooth and the drug use was basically rational.The sales amount of proton pump inhibitor was increased year by year.%目的:对医院口服抗消化性溃疡药物的用药现状和趋势作出客观评估,确保临床供应与管理。方法对解放军94医院2011-2013年口服抗消化性溃疡药物的用药金额、药理分类、用药排序及用药频度( DDDs)、限定日费用( DDC)等情况进行统计分析。结果口服抗消化性溃疡药物的销售金额逐年上升,其中质子泵抑制剂3年的销售金额均居首位,DDDs排名前2位的是兰索拉唑胶囊和奥美拉唑胶囊。结论2011-2013年我院口服抗消化性溃疡药物的用药趋势较平稳,使用情况基本合理,其中以兰索拉唑胶囊为首的质子泵抑制剂用药数量呈逐年增加趋势。

  5. Influence of antiseptic on the impurities in the preparation of dimercaptosuccinic labelled with {sup 99m}Tc; Influence des antiseptiques sur la presence d'impuretes dans la preparation de l'acide dimercaptosuccinique marque au 99mTc

    Energy Technology Data Exchange (ETDEWEB)

    Metaye, T.; Rosenberg, T.; Perdrisot, R. [CHU de Poitiers, Service de medecine nucleaire et biophysique, 86 (France)

    2010-07-01

    Purpose: antiseptic solutions are used to disinfect the rubber stoppers on the bottles of radiopharmaceutical. Small amounts of these solutions can pass into the bottle when the cap is pricked to inject the {sup 99m}Tc. After finding in a patient a suboptimal labelling using biseptin, we studied the effect of various antiseptics on the presence of impurities in the preparation of the dimercaptosuccinic acid labelled with {sup 99m}Tc ({sup 99m}Tc-D.M.S.A.). Conclusions: We recommend using 70% isopropanol or modified alcohol Cooper instead of biseptin to disinfect the D.M.S.A. bottles caps. (N.C.)

  6. Content Determination of Chlorhexidine Acetate in Wash-free Antiseptic Lotion by Coefficient Multiplying Ratio Spectrophotometry%系数倍率法测定免洗手消毒液中醋酸氯己定的含量

    Institute of Scientific and Technical Information of China (English)

    张青; 娄月芬; 唐跃年

    2012-01-01

    目的:测定免洗手消毒液中醋酸氯己定的含量.方法:采用系数倍率法,样品不经分离直接测定免洗手消毒液中醋酸氯已定的含量,测定波长为260、280 nm.结果:醋酸氯己定检测浓度线性范围为8~12 μg·mL-1(r=0.999 9),平均回收率为100.66%(RSD=0.21%).结论:本方法操作简便快速、结果准确,可用于该制剂的质量控制.%OBJECTIVE: To determine the content of chlorhexidine acetate in Wash-free antiseptic lotion. METHODS: The content of chlorhexidine acetate was directly determined at 260 nm and 280 nm by coefficient multiplying ratio spectrophotometry without preliminary separation. RESULTS: The linear range of chlorhexidine acetate was 8-12 μg·mL-1 (r=0.999 9) with an recovery of 100.66% (RSD=0.21%). CONCLUSION: The method is proved to be simple, rapid, accurate and applicable for the quality control of wash-free antiseptic lotion.

  7. EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY AND TOXICITY OF A HAND ANTISEPTIC GEL%一种手消毒凝胶杀菌效果与毒性试验观察

    Institute of Scientific and Technical Information of China (English)

    陈焕菊; 朱汉泉; 徐凤; 王可洲

    2011-01-01

    目的 观察一种手消毒凝胶的杀菌效果及毒性.方法 采用载体定量杀菌试验和动物毒性试验方法进行了实验室观察.结果 该手消毒凝胶主要杀菌成分为含体积分数60%乙醇与10 g/L葡萄糖酸氯己定组成.用该手消毒凝胶原液对载体上金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌分别作用0.5 min,杀灭对数值均>5.00;对白色念珠菌作用0.5 min,杀灭对数值>4.00.用该手消毒凝胶原液对志愿者手擦拭消毒1 min,对手部自然菌的平均杀灭对数值>1.00.该手消毒凝胶对小鼠急性经口毒性试验LD50 ﹥5 000 mg/kg(体重);其原液对家兔多次完整皮肤刺激试验积分为0;该消毒凝胶对小鼠骨髓嗜多染红细胞微核试验阴性.结论 该手消毒凝胶具有良好的杀菌效果,属于实际无毒级物质,对皮肤无刺激性,无致微核作用.%Objective To observe the germicidal efficacy and toxicity of a hand antiseptic gel. Methods Carrier quantitative germicidal test and animal toxicity test were used to carry out experimental observation. Results The main germicidal ingredients of the hand antiseptic gel contained 60% ethanol and 10 g/L chlorhexidine gluconate. The killing logarithm values of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa on carriers exposed to this hand antiseptic gel for 0.5 min were > 5.00 and that of Candida albicans exposed for 0.5 min was > 4.00. The average killing logarithm value of the natural bacteria on hand of volunteers exposed to this hand antiseptic gel for 1 min was > 1.00. The LD50 of mice acute oral toxicity was > 5 000 mg/kg · bw with the disinfection solution. Its cumulative score in skin irritation test by repeated contact with intact skin was 0. The mouse bone marrow polychromatic erythrocytes micronucleus test was negative.Conclusion The hand antiseptic gel has good germicidal efficacy. It belongs to practically non - toxic class. It has no irritation to

  8. Simultaneous Determination of 6 Antiseptics in Tobacco with LC-MS/MS Method%LC-MS/MS同时检测烟草中的6种杀菌剂

    Institute of Scientific and Technical Information of China (English)

    杨飞; 边照阳; 唐纲岭; 胡清源

    2012-01-01

    A method basing on high performance liquid chromatography-tandem mass spectrometry (LC-MS/ MS) was developed for determining the antiseptic residues in tobacco. The analytes extracted from tobacco samples were separated on C18 column (150 mm×2.1 mm×3 μm) using acetonitrile-water (containing 0.1% formic acid) as mobile phase. The antiseptics were determined by a tandem mass spectrometer with internal standard under multiple reaction monitoring (MRM)mode. The 145 domestic and imported tobacco samples were determined with this method. The results showed that: 1)The linear range was 5-200 ng/mL (/R2>0.999). The average recoveries ranged from 85.3% to 118.8% with the relative standard deviations (RSD) less than 7.7%. 2)No antiseptic residue was detected in 52 tobacco samples and 30 cigarette samples, which accounted for about 52% of all leave samples and 66.7% of all cigarette samples; all the mentioned antiseptics, except benomyl, were detectable in samples. This method was suitable for the fast determination of the residues of the 6 antiseptics in tobacco and tobacco products.%为测定烟草中的杀菌剂残留量,利用液相色谱-串联质谱(LC-MS/MS)建立了一种同时检测烟草中6种杀菌剂的方法:烟草样品提取液经处理后,在Atiantis dC18柱上以乙腈-水(含0.1%甲酸)为流动相进行分离,用LC-MS/MS在多反应监测(MRM)模式下测定,内标法定量.采用该方法测定了145个国内外烟草样品中6种杀菌剂农药的残留量.结果表明:①6种杀菌剂线性范围是5~200 ng/mL,线性关系良好(R2>0.999),平均回收率为85.3%~118.8%,相对标准偏差(RSD)小于7.7%,定量限在0.007~0.033 mg/kg之间.②所测样品中,有52个烟叶和30个卷烟样品中未检出任何杀菌剂农药,分别占所选烟叶和卷烟样品数的52%和66.7%;在6种杀菌剂农药中,除苯菌灵未检出外,其他5种农药均有检出.该方法适用于烟草及烟草制品中6种杀菌剂残留的快速检测.

  9. 气相色谱法测定果蔬汁饮料中6种防腐剂%Determination of 6 antiseptics in fruit and vegetable juice drink by gas chromatography

    Institute of Scientific and Technical Information of China (English)

    孙颖霞; 翁光灿; 倪炜华

    2011-01-01

    Concentrations of six antiseptics in fruit and vegetable juice were determined by gas chromatography (GC)/FID.The antiseptics are 2,4-hexadienoic acid, benzoic acid, dehydroacetic acid, methyl 4-hydroxybenzoate, ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate.Under the acidic condition, the sample was extracted with ether once, diluted with the multiple dilution method, and concentrated with little extraction solution and settled to permit with the acetone.The recoveries of all antiseptics were from 87.8% to 105.5% with the relative standard deviations between 0.8% and 3.1% when adding 10, 30, 50, 70 and 100 mg/kg standards to the blank samples.The detection limits were in the range of 0.4-1.0 mg/kg.The sample preparing of the method is simple and rapid, and the result of the method is reliable.%采用气相色谱仪(Agilent 6890N)配备火焰离子化检测器(FID)测定果蔬汁饮料中山梨酸、苯甲酸、脱氢乙酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯和对羟基苯甲酸丙酯6种防腐剂的含量.试样在酸性条件下,经乙醚一次提取后,采用稀释倍数法,移取少量提取液,浓缩近干,最后用丙酮定容.在10,30,50,70,100mg/kg5个加标水平下,6种防腐剂的回收率为87.8%~105.5%,相对标准偏差为0.8%~3.1%,检出限为0.4~1.0mg/kg.实验表明该方法样品前处理简便、快速,分析结果可靠.

  10. 爱尔碘皮肤消毒剂对生物膜阳性致病菌的体外杀菌试验%In vitro bactericidal experiment of ai'erdian skin antiseptic on positive pathogenic bacteria

    Institute of Scientific and Technical Information of China (English)

    王福兰

    2012-01-01

    目的 了解爱尔碘皮肤消毒剂对生物膜阳性致病菌的最小杀菌浓度.方法 将所有受试菌进行生物膜形成检测,再用对倍稀释法,以爱尔碘皮肤消毒剂不同浓度对生物膜阳性致病菌作用不同时间,观察杀菌效果.结果 金黄色葡萄球菌、大肠埃希菌和铜绿假单胞菌生物膜形成阳性率分别为54.3%、47.3%和57.0%;爱尔碘皮肤消毒剂在有效碘含量为9.203×10-4 mg/L时,作用1 min后,可将所有受试菌株全部杀灭.结论 爱尔碘皮肤消毒剂对生物膜阳性致病菌具有高效的杀菌性.%OBJECTIVE To study the minimum bactericidal concentration of ai erdian skin antiseptic on positive pathogenic bacteria. METHODS All the pathogenic bacteria producing biofilm were tested, then different concentrations of ai erdian skin antiseptic were applied to the biofilm-positive pathogens for difference period of time,the sterilization effects were observed. RESULTS The positive rates of the formation of biofilm of Staphylo-coccus aureus , Escherichia coli and Pseudomonasaeruginosa were 54. 3% , 47. 3% and 57. 0% ; All of the positive pathogenic bacteria were eradicated with the effective content of iodine of 9. 203 × 10-4 mg/L for lmin. CONCLUSION Ai erdian skin antiseptic is a kind of strong and effective disinfectant on biofilm positive pathogenic bacteria.

  11. 不同种类手消毒剂对脊髓灰质炎病毒灭活效果的实验观察%EXPERIMENTAL OBSERVATION ON THE INACTIVATION EFFICACY OF DIFFERENT HAND ANTISEPTICS ON POLIOVIRUS

    Institute of Scientific and Technical Information of China (English)

    王玲; 刘起展; 冷红英; 褚宏亮

    2013-01-01

    目的 观察不同种类手消毒剂对脊髓灰质炎病毒的灭活效果.方法 采用悬液定量试验法和载体定量试验法进行了实验室观察.结果 含有效碘4500 mg/L的聚维酮碘消毒液在清洁条件下作用10 min,对悬液内脊髓灰质炎病毒灭活对数值>4.00;在有机干扰物存在的条件下作用15 min,对悬液内脊髓灰质炎病毒灭活对数值>4.00.含体积分数70%乙醇与10 g/L过氧化氢的手消毒液在清洁条件下作用15 min,对悬液内脊髓灰质炎病毒灭活对数值>4.00;在有机干扰物存在的条件下作用30 min,对悬液内脊髓灰质炎病毒灭活对数值>4.00.含体积分数70%乙醇与10 g/L氯己定的手消毒凝胶作用3min,对载体上脊髓灰质炎病毒灭活对数值>4.00.结论 乙醇氯己定手消毒凝胶可以快速灭活载体上脊髓灰质炎病毒,聚维酮碘消毒液与乙醇过氧化氢手消毒液有效灭活悬液内脊髓灰质炎病毒需要10 min以上.%Objective To observe the inactivation efficacy of different hand antiseptics on poliovirus. Methods The suspension quantitative germicidal method and carrier quantitative germicidal method were used in this study to carry out the experimental observation. Results The average killing logarithm values of poliovirus in suspension was > 4. 00 exposed to the povidone iodine antiseptic containing available iodine 4 500 mg/L for 10 min under clean condition , While it was > 4.00 for 15 min under interfering substances condition. The average killing logarithm values of poliovirus in suspension was >4. 00 exposed to the 70% ( v/v) alcohol and 10 g/L hydrogen peroxid compound for 15 min under clean condition, however it was >4.00 for 30 min under interfering substances condition. The average killing logarithm values of poliovirus on carrier was >4.00 exposed to the 70% ( v/v) ethyl alcohol and 10 g/L chlorhexidine compound hand antiseptic gel for 3 min. Conclusion The compound hand antiseptic

  12. ε-聚赖氨酸在即食湿面条防腐中的应用%The antiseptic application of ε - Poly - L -lysine in wet ready - to - eat noodles

    Institute of Scientific and Technical Information of China (English)

    李维娜; 贾士儒; 谭之磊; 王洪翠; 张然

    2011-01-01

    ε - Poly - L - lysine ( ε-- PL) consists of 25 - 35 L - lysine residues with isopeptide linkage between it's a - carboxyl and e - amino group. Because of its.strong antimicrobial activity, safety and high thermostability, e - PL has been industrially produced and widely used as a natural food preservative. Wet ready - to - eat noodles are very susceptible to spoilage and have a short shelf life due to high moisture. The antiseptic effects of e - PL and e - PL with auxiliaries were researched in wet ready - to - eat noodles in this study. The results showed that e - PL was a potential preservative in wet noodle. In this study, soaking wet noodles into e - PL solution showed better antibacterial effects than other treatment method. The optimal concentration of e - PL alone was 0. 15% ; the antiseptic effect of e - PL with acetic acid was better than that of e -PL with alcohol. The optimal formulation was 0. 1% e - PL with 0. 15% acetic acid. This could extend the wet noodle shelf life to 10 d at 37 ℃. This study provides an experimental basis for the antiseptic application of e - PL in wet ready - to - eat noodles.%ε-聚赖氨酸(ε - Poly -L - lysine,ε-PL)是由25 - 35个L-赖氨酸单体在ε-氨基和α-羧基间脱羧形成的聚合物,具有很强的抑菌性、安全性和热稳定性,至今已工业化生产并作为一种天然食品防腐剂得到广泛的应用.即食湿面条因水分含量高,常温下易腐败变质,为延长其货架期,研究了ε - PL以及ε-PL与其他助剂复配对即食湿面条的抑菌效果.结果表明,e-PL对即食湿面条具有良好的抑菌效果.在本实验条件下,ε-PL对即食湿面条的较好处理方式为浸泡方式,单独使用ε-PL的最适浓度为0.15%; ε-PL与醋酸助剂复配的效果要好于酒精助剂,复合配方为:0.1%ε- PL和0.25%醋酸,经该配方浸泡过的即食湿面条在10 d内品质较好、总菌落数较低(37℃保藏),为ε-PL在即食湿面条防腐中的应用提供了实验依据.

  13. 纳米银医用抗菌敷料在烧伤患者治疗中的应用%The Application of Treatment Fire Burn Patients by Nanometer Silver Antiseptic Dressing

    Institute of Scientific and Technical Information of China (English)

    程景武

    2014-01-01

    目的:探讨纳米银医用抗菌敷料在烧伤患者治疗中的应用效果。方法:分析笔者所在医院2005年1月-2014年2月烧伤科收治的50例患者的临床资料,依据烧伤患者抗菌敷料不同进行分组,对照组20例和观察组30例。对照组采用磺胺嘧啶软膏进行创面的涂抹,观察组采用纳米银医用抗菌敷料进行处理,比较两组疗效。结果:观察组浅Ⅱ度烧伤患者伤后7d创面愈合率、平均愈合时间均优于对照组,两组比较差异均有统计学意义(P<0.05)。观察组深Ⅱ度烧伤患者伤后14 d创面愈合率、平均愈合时间均优于对照组,两组比较差异均有统计学意义(P<0.05)。结论:纳米银医用抗菌敷料应用在烧伤患者的治疗中可以明显提高愈合率,值得临床推广应用。%Objective:To explore fire burn patients by application nanometer silver antiseptic dressing. Method:50 cases clinical data of fire burn patients in department of burns our hospital from January 2005 to February 2014,which was to be divided into two group by different nanometer silver antiseptic dressing, control group 20 cases and detection group 30 cases. Daub of sulfadiazine ointment to the wound of control group,Nano silver antibacterial medical dressings for processing of detection group,the two group curative effect were detected. Result:The wound healing rate after fire burn 7 d、average healing time of detection group was better than control group in Superficial II degree burn,and the difference between the two groups was statistically significant(P<0.05).Wound healing rate after fire burn 14 d、average healing time of detection group was better than control group in deep second degree burn,and the difference between the two groups was statistically significant(P<0.05). Conclusion:The healing rate is obviously improved treatment fire burn patients by nanometer silver antiseptic dressing, which is worthy of promotion of clinical

  14. HPLC 法测定蛋白琥珀酸铁口服溶液中防腐剂的含量%Determination of antiseptic in iron proteinsuccinylate oral solutionby HPLC

    Institute of Scientific and Technical Information of China (English)

    刘存富

    2015-01-01

    Objective To establish an HPLC method for content determination of antiseptic in iron proteinsuccinylate oral solution. Methods HPLC with AichromBond-AQ C18 column was adopted.The mobile phase was acetonitrile-water (40∶60)at the flow rate of 1.0 mL·min -1 ,and the detective wavelength was 254 nm.The column temperature was 40℃.Results Sodium methylparaben and sodium propyl p-hydroxybenzoate isolated completely.The calibration curves were linear in the range from 0.873 mg·L -1 to 8.732 mg ·L -1 and from 0.296 mg·L -1 to 2.964 mg·L -1 (r >0.999).The average recoveries were 99.1% and 100.1% (RSD <2.0%). RSDs of precision were 0.04% and 0.22% respectively.Conclusions The method is convenient and rapid,which can be used for ac-curate and reliable quantitative detection of antiseptic in iron proteinsuccinylate oral solution.%目的:建立测定蛋白琥珀酸铁口服溶液中防腐剂含量测定方法。方法采用高效液相色谱法。使用 AichromBond-AQ C18柱,流动相为乙腈—水(40∶60),流速1.0 mL·min -1;检测波长254 nm,柱温40℃。结果口服液中的防腐剂对羟基苯甲酸甲酯钠和对羟基苯甲酸丙酯钠分离完全,检测浓度的线性范围分别为0.873~8.732 mg·L -1和0.296~2.964 mg·L -1,r均大于0.999;平均回收率分别为99.1%和100.1%(RSD 均小于2.0%),精密度 RSD 分别0.04%和0.22%。结论该方法简单快捷,可用于新药蛋白琥珀酸铁口服液防腐剂的定量检测,结果准确可靠。

  15. 硝酸咪康唑乳膏中防腐剂测定方法的建立%Construction of a Method to Determine the Antiseptics in Miconazole Nitrate Cream

    Institute of Scientific and Technical Information of China (English)

    刘燕

    2015-01-01

    目的:建立测定硝酸咪康唑乳膏中防腐剂含量的高效液相色谱法。方法色谱柱为Diamonsil C18柱(250 mm ×4.6 mm,5μm),以0.05 mol/L磷酸二氢钾-乙腈(72∶28)为流动相,检测波长为235 nm。结果苯甲酸、山梨酸、羟苯乙酯的检出限分别为4.77,5.26,10.24 ng,进样量线性范围分别12.5~200μg,5~80μg,25~400μg,相关系数分别为0.99996,0.99997,0.99991(n=6)。结论该方法操作简单、重复性好、检测结果准确,能更好地检测硝酸咪康唑乳膏中防腐剂的含量。%Objective To establish a method to determine the antiseptics in Miconazole Nitrate Cream.Methods The HPLC analysis was performed on Diamonsil C18(250 mm ×4.6 mm,5 μm)column;the mobile phase consisted of 0.05 mol/L monopotassium phosphate and acetonitrile(72 ∶28);the detective wavelength was 235 nm.Results The limit of detection was 4.77,5.26 and 10.24 ng for ben-zoic,sorbic acid and ethylhydroxybenzoate respectively.It had good linear relationship at the range of 12.5~200 μg,5~80 μg,25 ~400 μg respectively and the correlation coefficient was 0.999 96,0.999 97 and 0.999 91(n=6)for benzoic,sorbic acid and ethylhy-droxybenzoate respectively.Conclusion The method is simple,good in repeatability,accurate for quantitation and can be used for deter-mining the antiseptics in Miconazole Nitrate Cream.

  16. Screening of Germicides. to Kill Microbes from Reservoirs that can Corrupt Steel and Testing of Antiseptic Paints%水库中致腐蚀微生物杀菌剂的筛选及抗腐蚀涂料的检测

    Institute of Scientific and Technical Information of China (English)

    崔恒林; 仓一华; 陆玲; 孙延涛; 孙红尧; 李震; 朱秀娟

    2000-01-01

    We examined the microorganisms in reservoir water and obtained some corrupted the steel in reservoir, with multi-tube fermentation method. Incubating these microbes in different media in which there are different germicides with different concentrations, we screened more effective germicides to produce antiseptic paints. Discs made of the antiseptic paints were used to do bacteriostatic experiments. Long-time experiments were also done. The result showed that the No.22 germicide and its antiseptic paint are more effective materials, which can prevent the steel in reservoir from being corrupted.%采用多管发酵培养法检测水样中致腐蚀菌并富集出测试菌群.设置杀菌剂浓度梯度,筛选出有效的杀菌剂.对不同配比及含不同杀菌剂的树脂涂层进行耐腐蚀及杀菌效果检测.并对其中效果最好的22号杀菌剂涂层进行了长效性实验和耐菌性实验.结果表明含22号杀菌剂的抗菌涂料是一种很好的抗水库中微生物腐蚀的材料.

  17. The Present Situation and Prospect of Nature Food Antiseptic and Preservation Agent%天然食品防腐保鲜剂的发展现状及前景

    Institute of Scientific and Technical Information of China (English)

    张海霞

    2015-01-01

    The natural food preservative agent is an additive in food processing, which plays an extremely important role in food preservation. However, it is not reasonable to use of natural food additives, this will have a bad impact on food quality, and food safety is threatened. Therefore, according to the development of natural food preservative agent, according to the actual situation of the development of natural food preservative, combining with the actual situation of social development, this paper analyzed its development prospect, and the relevant opinions and suggestions of the use of natural food antiseptic was put forward.%天然食品防腐保鲜剂是食品加工中必须加入的添加剂,其对食品保鲜起着极为重要的作用。但是,不合理地使用天然食品添加剂,会对食品质量造成不良的影响,使得食品的安全受到一定的威胁。因此,针对天然食品防腐保鲜剂的发展进行具体分析,根据天然食品防腐保鲜剂发展的实际情况,结合社会发展实际,分析其发展的前景,并对天然食品防腐保鲜剂的使用提出相关的意见和建议。

  18. Screening of Antitumor and Antiseptic Activities of Endophytic Fungus from White Eaglewood (Aquilaria sinensis)%白木香内生真菌抗肿瘤抗菌活性的筛选研究

    Institute of Scientific and Technical Information of China (English)

    李冬利; 张庆波; 陈玉婵; 李浩华; 潘清灵; 高晓霞; 章卫民

    2009-01-01

    White eaglewood (Aquilaria sinensis) is a rare and endangered medicinal plant endemic of China. Endo-phytic fungi from the plant with antitumor and antiseptic activities in the extract of fermentation broth were screened. The results indicated that there were 6 strains exhibited inhibition against the proliferation of tumor cell SF-268 at the rate above 90%, account for 31.6% of total strains isolated; And another 16 strains possessed a certain bacterial in-hibition against Staphylococcus aureus, account for 84.2% of the total strains isolated, among them 9 strains had fairly strong inhibition against bacteria, one strain indicated fairly strong inhibition against Candida albicans, two strains showed a certain inhibition activity against AspergiUus niger. And all the strains isolated had not obvious inhibition ac-tivity against E. coli.%白木香[Aquilaria sineusis(Lonr.)Gilg]是我国特有的珍稀濒危药用植物.对已分离得到的白木香内生真菌的发酵液提取物的抗肿瘤抗菌活性进行了筛选.结果表明,对肿瘤细胞SF-268增殖的抑制率在90%以上的菌株有6株,占总菌株数的31.6%.其中有16株对金黄色葡萄球菌具有一定的抑菌活性,占总菌株教的84.2%,有9株表现较强的抑菌活性.另外有1株对白色念珠菌表现较强的抑菌活性;有2株对黑曲霉表现一定的抑菌活性.所有菌株对大肠埃希菌都没有表现明显的抑菌活性.

  19. High pressure antiseptic chemical monitoring different test package of test effect comparison%高压灭菌化学监测不同测试包测试效果比较

    Institute of Scientific and Technical Information of China (English)

    关瑞锋; 杨晓霞; 张玉琴; 张淑北; 杨燕; 安云凤; 邓晓华

    2008-01-01

    Objective Installs PCD and the self-made test cloth wrapper test effect compared with the housing process interpellation.Methods The maintenance test research technique carries on the grouping com-parison to 200 high-pressured antiseptic circulation chemical monitoring,the experimental group uses the hous-ing process interpellation to install PCD,in this equipment puts in chemistry indicator card 1; The control group uses the self-made test cloth wrapper,Bao Neifang has chemistry indicator card 1,total 5 packages.Results Two groups experiment the qualified rate and the human factor,the equipment factor are remarkable to the experi-mental influence contrast difference,P <0.01.Conclusion The housing process interpellation installs the PCD qualified rate to be higher than the self-made test cloth wrapper obviously,it may reduce the hnman factor to the experiment the influence,may take must implants the load to allow to pass the important basis,active control nusocomial infection occurrence.%目的 比较腔体型过程质询装置PCD和自制测试布包测试效果.方法 应用试验研究方法对200次高压灭菌循环化学监测进行分组对照,实验组采用腔体型过程质询装置PCD,该装置内放入化学指示卡1枚;对照组采用自制测试布包,包内放有化学指示卡1枚,共计5个包.结果 两组试验合格率及人为因素、设备因素对试验影响的对比差异显著(P<0.01).结论 腔体型过程质询装置PCD合格率明显高于自制测试布包,它可减少人为因素对试验的影响,可作为非植入物负荷放行重要依据,有效控制医院感染的发生.

  20. 不同环境下二氧化氯消毒剂的消毒效果及其稳定性研究%The research of disinfectant effect and stabilization of chlorine dioxide antiseptic in different environments

    Institute of Scientific and Technical Information of China (English)

    张庆华; 姬素霞; 胡金花; 闫丽; 张鹏; 胡建和; 徐彦召

    2014-01-01

    为了研究不同环境下二氧化氯的消毒效果及其稳定性,试验以大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、白色念珠菌为测试菌株,采用菌悬液定量杀菌试验、压缩雾化法和平板计数的方法,对二氧化氯的杀菌效果进行试验。结果表明:在菌液密度为5×106 CFU/mL的条件下,90 mg/L的二氧化氯作用5 min,即可对测试菌株达到100%的杀灭效果。不同的pH值、温度及不同浓度有机物存在的条件下,二氧化氯同样具有极强的消毒效果。雾化的二氧化氯对空气自然菌及雾化菌液气溶胶的杀灭效果同样可以达到100%。试验表明二氧化氯作为一种高效、稳定的广谱消毒剂具有良好的杀菌效果。%To research the disinfetant effect and stabilization of chlorine dioxide in different environments,the antibacterial activity of chlorine dioxide to Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Manidia albican was tested by quantitative germicidal test,compression atomizing effect and plate counting method.The results showed that chlorine dioxide at 90 mg/L could kill all the bacteria in 5 min.Chlorine dioxide exhibits widely antiseptic properties in multi-environment,despite of the pH value,temperature and organic matter.Chlorine dioxide in gaseous also had the forceful sterilizing effect to the normal air and the theaerosol of bacterial suspension.It indicated that chlorine dioxide with the quality of efficient and stable have the excellent antibacterial activity as an disinfectant.

  1. 不同防腐剂及不同添加方式对血豆腐抑菌效果的研究%Study on Bacteriostatic Effect of Different Antiseptics and Different Adding Ways on Blood Tofu

    Institute of Scientific and Technical Information of China (English)

    崔瑞颖; 张志胜; 淑英; 崔波; 王桓; 李叶

    2014-01-01

    The antibacterial effects of different antiseptics of potassium sorbate and Sodium diacetate which add by different ways were studied. The results showed Using the same preservatives , the antibacterial effect by soaking to blood tofu than adding directly is better and the difference is significantly. By the way of soaking to blood tofu, the antibacterial time solution extended 32 h by the optimal concentration 4%of potassium sorbate solution than 0.2%of sodium diacetate, the difference is significantly. By the way of adding directly to blood tofu, the bacteriostatic effect have no differences by the most appropriate to adding 0.25%of sodium diacetate and 4%of potassium sorbet. So, choosing the concentration of 4%potassium sorbet solution to blood tofu can achieve the best antibacterial effect.%对不同浓度的山梨酸钾、双乙酸钠及不同的添加方式对血豆腐的抑菌效果进行了研究。结果表明:使用相同的防腐剂,采用溶液浸泡的方法比直接将防腐剂添加至血豆腐中,抑菌效果更好,并差异性显著;采用防腐溶液浸泡血豆腐的方式,最适浓度4%的山梨酸钾溶液比0.2%的双乙酸钠溶液抑菌时间延长了32 h,效果差异显著;采用血豆腐中直接添加的方式,最适添加量0.25%的双乙酸钠及4%的山梨酸钾抑菌效果差异不显著。因此,选择4%浓度的山梨酸钾溶液对血豆腐进行浸泡,可以达到最好的抑菌效果。

  2. Observation on disinfection effect and toxicity of hand antiseptic agent in healthcare settings%医院手部消毒液消毒效果与毒性观察

    Institute of Scientific and Technical Information of China (English)

    林立旺; 陈路瑶; 陈明中; 吴心勤

    2011-01-01

    目的:观察医院手部消毒液消毒效果与毒性情况.方法:依据(2002年版)对其进行了实验室定量杀菌试验、手现场消毒试验与毒理学试验等.结果:该消毒液对金黄色葡萄球菌、大肠杆菌、绿脓杆菌作用0.5 min杀灭对数值均>5.00,对白色念珠菌作用0.5 min杀灭对数值>4.00;对30人次志愿受试者手上自然菌消毒作用1 min平均杀灭对数值>1.00,上述消毒试验各项指标均达到消毒合格要求;大鼠急性经口毒性试验结果为LD50>5000 mg/kg体重;微核试验结果为阴性;多次皮肤刺激试验结果为无刺激性.结论:该消毒剂具有良好的杀菌效果,杀菌谱广、作用快,且毒性低,为实际无毒级,可用于医护人员卫生手消毒和外科手消毒.%Objective:Observation on disinfection effect and toxicity of hand antiseptic agent in healthcare settings. Methods:Base on "Technical Stsndard For Disinfection" ( 2002 ), quantitative germicidal test, hand field trial, toxicity test were carried out.Results: The results showed that the killing log values of Staphylococcus, Escherichia coli, Bacillus aeruginosus exposed to the disinfectant solution for 0. 5 min were all > 5.00, Candida albicans exposed to the disinfectant solution for 0.5 min is > 4.00 and the mean kill logrithm of hand natural bacteria for thirty volunteer with one min is > 5.00 too, all the disinfect test index reach the disinfection requirements. The acute oral toxicity test in rats is LD50 > 5000 mg/kg;Micronucleus test result were negative; repeated skin irritation test showed non -irritaion. Conclution: The disinfect had good bactericidal effect, wide spectrum sterilization,quick action,and low toxicity, which is the actual non- toxic level. It can use for the hand hygiene and surgery disinfection.

  3. 畜禽生产区常用消毒剂杀菌效果监测方法探讨%An Inquiry into the Detection Method of the Antiseptic Effect of Commonly Used Disinfectant in Livestock and Poultry Production Area

    Institute of Scientific and Technical Information of China (English)

    周志远; 黄华英; 曾培林

    2011-01-01

    本试验以菌毒敌、烧碱等常用消毒剂为代表,监测它们在人车进出畜禽生产区消毒池中的杀菌抑菌效果,对已知大肠杆菌的最小抑菌浓度及其有效浓度持续时间的变化情况,测得烧碱的最小抑菌浓度为0.25%、菌毒敌为0.16%,按菌毒敌使用说明配制的浓度在消毒池中持续到第九天仍有较好的抑菌效果。%With the commonly used disinfectant,such as Jun-Du-Di and caustic soda,as examples,this experiment detect their antiseptic effect in livestock and poultry areas and the minimum inhibitory concentration(MIC) against ascherichia,together with the change of the lasting time of effective concentration.The result is that the MIC of caustic soda is 0.25%,that of Jun-Du-Di is 0.16%,and the concentration in accordance with the instructions of Jun-Du-Di lasts its antiseptic effect to the 9th day.

  4. Antiseptic skin agents for percutaneous procedures.

    Science.gov (United States)

    Lepor, Norman E; Madyoon, Hooman

    2009-01-01

    Infections associated with percutaneously implanted devices, such as pacemakers, internal cardiac defibrillators, and endovascular prostheses, create difficult and complex clinical scenarios because management can entail complete device removal, antibiotic therapy, and prolonged hospitalization. A source for pathogens is often thought to be the skin surface, making skin preparation at the time of the procedure a critical part of minimizing implantation of infected devices and prostheses. The most common skin preparation agents used today include products containing iodophors or chlorhexidine gluconate. Agents are further classified by whether they are aqueous-based or alcoholbased solutions. Traditional aqueous-based iodophors, such as povidone-iodine, are one of the few products that can be safely used on mucous membrane surfaces. Alcohol-based solutions are quick, sustained, and durable, with broader spectrum antimicrobial activity. These agents seem ideal for percutaneous procedures associated with prosthesis implantation, when it is critical to minimize skin colony counts to prevent hardware infection.

  5. [New antiseptics and disinfectants in surgery (2)].

    Science.gov (United States)

    Pkhakadze TYa; Bogomolova, N S; Vinogradova, L N

    1996-01-01

    Various forms of plivasept, an agent produced by Pliva (Croatia), were studied in the laboratory and clinic and found to be highly effective. For the prevention of intrahospital infections the use of 0.02% and 0.05% water solutions of plivasept without PAV is advisable as an antiseptec for inyracavitary administration, 0.05% water solution of plivasept for disinfection of surfaces and equipment, 0.5% alcohol plivasept solution for disinfection of instruments, a plivasept tincture for surgical treatment of the skin on the hands and the injection and operative fields, and foam-forming plivasept for hygienic treatment of the hands.

  6. In vitro activity of antifungal and antiseptic agents against dermatophyte isolates from patients with tinea pedis Atividade in vitro de antifúngicos e anti-sépticos frente a dermatófitos isolados de pacientes com tinea pedis

    Directory of Open Access Journals (Sweden)

    Maria Magali Stelato Rocha Soares

    2001-06-01

    Full Text Available The in vitro activity of antifungal and antiseptic agents were evaluated against dermatophytes isolated from patients with tinea pedis. The antifungals studied were: ciclopirox olamine, cetoconazole, tolciclate and terbinafine, and the antiseptics were: povidine iodine (PVPI, propolis, Fungol®, Andriodermol®, and boric acid. The minimum inhibitory concentration (MIC or the minimal dilution concentration (MDC was determined by an agar dilution method using modified yeast nitrogen agar base, and the minimum fungicidal concentration (MFC or minimum fungicidal dilution (MFD was determined with subcultures on Sabouraud dextrose agar. All drugs studied were active against the dermatophytes at lower concentrations than those used in products and/or pharmaceutical preparations for topical use. Some antifungal agents, mainly terbinafine and tolciclate, presented higher efficacy than the other drugs, with lower MICs and MFCs values. It was concluded that the use of these antiseptic drugs represent an excellent alternative for the topical treatment of tinea pedis. For the treatment of severe cases these are the antifungal agents of choice.A atividade in vitro de antifúngicos e anti-sépticos foram avaliadas frente a dermatófitos isolados de pacientes com tinea pedis. Os antifúngicos estudados foram: ciclopirox olamine, cetoconazol, tolciclato e terbinafina, e os anti-sépticos foram: iodo povidine (PVPI, própolis, Fungol®, Andriodermol®e ácido bórico. A concentração inibitória mínima (CIM ou a diluição inibitória mínima (DIM foi determinada pelo método de diluição em ágar utilizando "yeast nitrogen" base modificado, e a concentração fungicida mínima (CFM ou diluição fungicida mínima (DFM foi determinada por subcultura em Saboraud dextrose ágar. Todas as drogas estudadas foram ativas frente aos dermatófitos em concentrações menores do que as utilizadas em produtos e/ou preparações farmacêuticas para uso tópico. Alguns

  7. 壳聚糖抗菌成膜喷雾剂联合多功能治疗仪治疗中度痤疮临床观察%Clinical Observation of Chitosan Antiseptic Material Combined with Multifunctional Therapeutical in the Treatment of Moderate Acne Vulgaris

    Institute of Scientific and Technical Information of China (English)

    侯艳霞; 夏华; 韩旭; 李定超; 蒋靖

    2014-01-01

    Objective To evaluate the ef icacy of chitosan antiseptic material combined with multifunctional therapeutical in the treatment of moderate acne vulgaris. Methods The 50 patients were randomly divided into two groups, each group included 25 patients. The treatment group was given chitosan antiseptic material combined with multifunctional therapeutical while the control group was only given multifunctional therapeutical. The therapeutic ef ect was observed at the end of the 4th week after the treatment. Results After 4 weeks,the quantity of skin eruption in both groups was apparently lower than before but that of treatment group was apparently lower than that in control group.Besides, the ef ective rate in the treatment group (84.00%) was higher than that in the control group (56.00%).The dif erences were statistical y significant ( <0.05).No side effects were observed. Conclusion Chitosan antiseptic material combined with multifunctional therapeutical is ef ective in the treatment of moderate acne vulgaris with lit le adverse reaction.%目的:评价壳聚糖抗菌成膜喷雾剂联合多功能治疗仪治疗中度痤疮的临床疗效。方法将入选的50例中度痤疮患者随机分为两组,各25例。治疗组予壳聚糖抗菌成膜喷雾剂喷涂联合多功能治疗仪治疗,对照组仅予多功能治疗仪治疗,均在治疗4w后判定临床疗效。结果治疗4w后,治疗组和对照组的皮损总数均明显低于治疗前,而且治疗组明显少于对照组,治疗组有效率(84.00%)优于对照组(56.00%),差异均有统计学意义(<0.05)。两组患者也均未见明显的不良反应。结论壳聚糖抗菌成膜喷雾剂联合多功能治疗仪治疗中度痤疮有较好的临床疗效,且无明显不良反应。

  8. 万金消毒液与全自动内镜清洗消毒机对消化内镜消毒效果比较%Effects of Wanjin antiseptic germicides and fully automatic endoscope cleaning and sterilization machine on sterilization of endoscopes : a comparative study

    Institute of Scientific and Technical Information of China (English)

    刘奉; 宋燕玉; 毕楠楠

    2012-01-01

    目的 观察万金消毒液不同消毒作用时间对细菌、乙型肝炎病毒(HBV)与幽门螺杆菌(Hp)感染患者所使用的消化内镜消毒效果,并与明泰科全自动内镜洗消机进行对照,探讨万金消毒液对细菌、HOV与Hp感染患者所使用的消化内镜消毒的实用性.方法 研究组用含有效氯2 g/L万金消毒液对清洗后的消化内镜作循环冲洗后随机浸泡5 min和10 min,消毒后内镜采样做细菌、Hp培养及RT-PCR检测HBV-DNA、放射免疫法测定HBsAg定量,比较不同消毒作用时间对HBV与Hp感染患者所使用的消化内镜的消毒效果;对照组用明泰科全自动内镜洗消机对HBV与Hp感染患者所使用的消化内镜进行消毒,对消毒后内镜采样做细菌、Hp培养及HBV-DNA、HBsAg检测.结果 万金消毒液浸泡消毒内镜5、10 min对细菌、Hp消毒合格率均为100.00%,对HBV 5 min内镜检测出HBV-DNA阳性,合格率为93.33%,浸泡消毒10 min合格率为100.00%;对照组明泰科全自动洗消机消毒的内镜均未检出细菌、Hp及HBV,合格率均100.00%;万金医用器械消毒液对HBV与Hp感染患者所使用的消化内镜浸泡消毒10 min与明泰科全自动洗消机的消毒效果差异无统计学意义.结论 万金消毒液可作为HBV与Hp感染患者的消化内镜消毒的首选消毒剂之一.%OBJECTIVE To investigate the practicability and observe the degermation effect of Wanjin antiseptic germicide for different time, compared with Minntech DSD201 automatic endoscopy sterilizer, on digestive endoscopies used by the patients of HBV and Helicobacter pylori ( Hp) infections. METHODS The digestive endoscopies of the research group were randomly disinfected by immersion for 5min or l0min after they were iteratively rinsed in Wanjin antiseptic germicide of freshly prepared and 2g/L active chlorine-included, then the samples gained from these pre and post-sterilized endoscopies were treated by germiculture, Hp-culture, RT

  9. Synthesis of Ag-TiO2/CNTs nanopartical composites and their photocatalytic activity and antiseptic property%载银 TiO2/碳纳米管复合材料的制备及其催化杀菌性能

    Institute of Scientific and Technical Information of China (English)

    李君建; 李巧玲

    2015-01-01

    采用溶胶-凝胶法,在450℃下煅烧制备载银 TiO2/碳纳米管纳米复合材料;采用 XRD、SEM 以及 N2吸脱附对样品的晶体结构及样品微观形貌进行表征;通过 UV-vis 漫反射光谱表征了复合材料对紫外可见光的吸收性能;以甲基橙为模型污染物,评价了样品紫外光光催化降解甲基橙的活性,以大肠杆菌和枯草芽孢杆菌为菌种,采用抑菌圈法表征样品的杀菌性能。结果表明,载银 TiO2/碳纳米管纳米复合材料比纯 TiO2纳米颗粒有更好的紫外光光催化活性,其中掺碳纳米管(CNTs)1%(质量分数)的效果最好,紫外光照射150min,降解率达到76.5%;载银 TiO2/碳纳米管纳米复合材料比 TiO2纳米颗粒、Ag 纳米粒子、CNTs(纯化处理后)的杀菌效果都要好很多,其中掺 CNTs 10%(质量分数)的样品的杀菌效果最好,抑菌圈直径达到25.8mm。%Ag-TiO2/CNTs nanopartical composites were synthesized by sol-gel method,and then obtained samples were thermally treated at 450℃. The crystal morphology and the micro-surface structures were characterized by XRD,SEM and low temperature N2 adsorption-desorption. Absorbency of composites was analyzed by UV-vis diffuse reflection spectra. Under artificial UV-light irradiation,the degradation of methylorange was used to evaluate the photo-catalytic activity of Ag-TiO2/CNTs nanopartical composites. E.coli and bacillus subtilis were used to characterize antiseptic property by bacteriostatic circle method. The result showed that the photocatalytic activity of Ag-TiO2/CNTs nanopartical composites was higher than that of pure TiO2,and Ag-TiO2/CNTs nanoparticles with 1% (mass fraction) CNTs showed the best photocatalytic activity. The degradation rate is 76.5% exposuring to ultraviolet light about 150min. Ag-TiO2/CNTs nanopartical had much better antiseptic property than TiO2nanopartical,Ag nanoparticles, CNTs (purified),and Ag-TiO2/CNTs nanopartical

  10. Macroscopic and microscopic study of tissue response to oral antiseptics and its influence on carcinigenesis Estudo macro e microscópico da resposta tecidual frente ao uso de anti-sépticos bucais e sua influência na carcinogênese

    Directory of Open Access Journals (Sweden)

    Camila Lopes Cardoso

    2005-09-01

    Full Text Available Studies have related the action of alcohol on the oral mucosa as a promoter of carcinogenesis, once most oral antiseptics contain alcohol. Its utilization for mouthrinses from 30 to 60 seconds, as indicated on the labels, yields a longer-lasting topical action when compared to the intake of alcoholic beverages. This study aimed at conducting a macroscopic and microscopic analysis of the tissue response of tongue mucosa of hamsters to daily topical applications of antiseptics (Anapyon, Listerine, Oral B during 13 and 20 weeks, following the methodology for carcinogenesis investigation developed by the Discipline of Pathology of Bauru Dental School, University of São Paulo. After sacrificing the animals, their tongues were removed and fixed on 10% formalin. Macroscopic examination did not reveal significant alterations, and the specimens were processed by routine histotechnical procedures for HE staining. Three serial sections of each tongue were evaluated, and characteristics related to epithelial hyperkeratinization, atrophy, hyperplasia and dysplasia were organized in tables. Despite the observation for moderate dysplasia in one case in the Anapyon 20 week group, the further results were very similar to the control group (saline solution, eliminating the need of comparative statistical tests. By means of such methodology for testing the carcinogenesis-initiating action, it was concluded that oral antiseptics are unable to trigger the development of neoplasms.Estudos associam a ação do álcool na mucosa bucal como promotora da carcinogênese e a maioria dos anti-sépticos bucais contém álcool. Sua utilização com bochechos de 30 a 60 segundos indicados nos frascos possui ação tópica mais duradoura em comparação com a ingestão de bebidas alcoólicas. Este estudo objetivou analisar macro e microscopicamente a resposta tecidual da mucosa lingual de hamsters após aplicações tópicas diárias de anti-sépticos (Anapyon, Listerine, Oral

  11. Evaluation of concentration and antiseptic effect of sodium hypochlorite in cattle foot bath/ Avaliação da concentração e do efeito sanitizante do hipoclorito de sódio em pedilúvio para bovinos

    Directory of Open Access Journals (Sweden)

    Maria Ivete de Moura

    2007-07-01

    Full Text Available This study aimed to evaluate the antiseptic effect of 2% and 4% sodium hypochloride solutions in footbath for bovines, considering parameters as stability during storage and use, action on mesophilic aerobic and anaerobic microorganisms and direct effects on tissue. Healthy bovines passed by a footbath, once a day, during five days. At the end of passages, samples of each solution were harvested for physiochemical and microbiological analysis. The first samples were obtained immediately after the solution deposition in footbath. Student t Test (p= 0,05% was applied for data analysis. It was observed that 2% and 4% solutions didn’t show significant differences on aerobic and anaerobic microorganisms counting, suggesting that solutions have the same antiseptic effect. In both solutions, the pH remained steady. Free chlorine concentrations showed levels equivalent to zero from the third day. Clinical signs of injury weren’t observed in interdigital skin and others digital regions. Thus, these results allow us to conclude that sodium hypochloride solution in 2% and 4% dilutions, when they are used in footbath, have antiseptic effect in bovine digits, however, the renewal must be after 48 hours.O objetivo deste trabalho foi avaliar o efeito sanitizante do hipoclorito de sódio em soluções a 2% e 4% em pedilúvio para bovinos, empregando como parâmetros sua estabilidade durante estocagem e uso, ação sobre microrganismos aeróbios mesófilos e anaeróbios e o efeito direto sobre o tecido animal. Durante cinco dias, bovinos saudáveis passaram em um pedilúvio, em intervalos de 24 horas. Ao final da passagem eram colhidas amostras de cada solução para análises físico-químicas e microbiológica sendo a primeira colheita realizada imediatamente após a deposição no pedilúvio. Aplicou-se o teste t de Student (p=0,05% para análise dos resultados. As soluções a 2% e a 4% não apresentaram diferença significativa, quanto à contagem de

  12. Avaliação da atividade anti-séptica de extrato seco de Stryphnodendron adstringens (Mart. Coville e de preparação cosmética contendo este extrato Antiseptic activity evaluation of the dry extract from Stryphnodendron adstringens (Mart. Coville and cosmetic preparation

    Directory of Open Access Journals (Sweden)

    Tatiana M. Souza

    2007-03-01

    Full Text Available As espécies pertencentes ao gênero Stryphnodendron são conhecidas como "barbatimão" e são nativas do cerrado brasileiro. Possuem taninos como metabólitos secundários primordiais de suas cascas apresentando, entre outras, atividade antimicrobiana e cicatrizante. A concentração bactericida mínima (CBM do extrato seco das cascas de Stryphnodendron adstringens frente a duas bactérias Gram-positivas e uma bactéria Gram-negativa foi determinada pela técnica de diluição em tubos. A atividade antimicrobiana do extrato seco e a atividade anti-séptica de sabonete líquido contendo o extrato seco foram avaliadas pelo método de difusão em ágar. O extrato apresentou valores de concentração bactericida mínima de 50 mg/mL frente a Staphylococcus aureus e 75 mg/mL contra Staphylococcus epidermidis e Escherichia coli e no teste de difusão em ágar, S. aureus apresentou maior sensibilidade ao extrato seco que as outras bactérias. O sabonete líquido mostrou maior eficiência na atividade anti-séptica contra as bactérias testadas na concentração de 100 mg de extrato/mL de sabonete.The species of the genus Stryphnodendron are known as "barbatimão" and are native from Brazilian "cerrado". They present tannins as main secondary metabolites of their barks and show antimicrobial and cicatrisation activity among others. The minimum bactericidal concentration (MBC of the dry extract from the barks of Stryphnodendron adstringens against two Gram-positive and one Gram-negative bacteria was done using methodology of broth dilution in tubes. The antimicrobial activity of the dry extract and the antiseptic activity of the liquid soap containing that dry extract were evaluated by agar difusion method. For the extract were obtained the minimum bactericidal concentration values of 50 mg/mL against Staphylococcus aureus and 75 mg/mL against Staphylococcus epidermidis and Escherichia coli and by agar difusion, S. aureus showed more sensibility to the

  13. Interpretation of New Standard of JC/T897-2014:Antiseptic Function of Antibacterial Ceramic%《抗菌陶瓷制品抗菌性能》(JC/T 897-2014)新版标准解读

    Institute of Scientific and Technical Information of China (English)

    王静; 冀志江; 赵春艳; 王晓燕

    2015-01-01

    本文对《抗菌陶瓷制品抗菌性能》(JC/T897-2014)新修订版标准进行了解读.从目前抗菌陶瓷产业现状、标准修订意义、详细技术要求和试验方法的确定、试验验证情况等几方面进行了详细介绍.让从业人员充分了解认识这个新修订标准的具体内容.%This paper interpreters the newly revised standard of JC/T897-2014: Antiseptic function of antibacterial ceramic and expounds the current situation of antibacterial ceramic industry, the significance of standard revision, the detailed technical requirements and test methods, test condition and so on, providing specific contents for practitioners to fully understand the newly revised standard.

  14. 抗感染中心静脉导管在减少导管相关性感染和细菌定植中的临床随机对照研究%Reduction of bacterial colonization and catheter-related infection with antiseptic central venous catheter: a randomized controlled clinical trial

    Institute of Scientific and Technical Information of China (English)

    杨钧; 程芮; 公静; 奚晶晶

    2011-01-01

    目的 比较用磺胺嘧啶银和洗必泰抗感染的中心静脉导管(CVC)与普通导管在减少细菌定植和导管相关性感染方面的差异.方法 采用前瞻性、随机对照分析方法,对2007年6月-2009年6月武警总医院ICU收治的70例需要深静脉置管患者进行随机分组,留置双腔抗感染CVC(抗感染组,n=28)和双腔普通CVC(对照组,n=42),两组采用相同的穿刺部位和消毒护理措施,观察记录患者的一般情况、APACHE Ⅱ评分、导管留置天数、拔除导管原因等,通过对导管尖端及皮下部分的细菌半定量培养、血培养以及局部皮肤有无红肿、脓性分泌物来确定有无导管定植(CBC)、导管相关性血流感染(CRBSI)和局部导管相关感染(CRI).采用SPSS 11.5软件包进行统计分析,CVC留置时间与细菌定植和感染发生率采用Kaplan-Meier曲线分析,组间差异比较用Log-rank检验.结果 抗感染组由于感染原因被迫提前拔管1例,占3.6%,对照组9例,占21.4%,比较差异有统计学意义(x2=5.143,P 15 CFU was found by semi-quantitative roll-plate technique from a proximal or distal catheter segment. A catheter-related infection ( CRI) was defined as a colonized catheter with local signs of inflammation. A catheter-related bloodstream infection ( CR-BSI) was defined as a colonized catheter with isolation of the same organism from the patient' s blood with accompanying clinical signs of infection. SPSS 11.5 software was used for statistical analysis. Kaplan Meier curve was used to evaluate the association between CVC retention time and bacterial colonization or infection, and Log-rank test was performed to compare between the groups. Results CVC was removed from 3.6% (1/28) patients of antiseptic group and 21.4% (9/42) patients of control group because of infection (x2 = 5. 143, P 2 weeks, the colonization and infection will increase significantly in both standard or antiseptic CVC, so to shorten the insertion time is an

  15. Study of broad-spectrum antibiotics and antiseptics resistance genes in Acinetobacter baumannii strains isolated from burned patients%鲍曼不动杆菌烧伤分离株广谱抗生素及消毒剂耐药基因研究

    Institute of Scientific and Technical Information of China (English)

    程华莉; 潘宇红; 黄璇; 吕国忠; 朱婕; 糜祖煌; 张烽

    2011-01-01

    目的 研究鲍曼不动杆菌烧伤分离株对广谱抗生素的耐药性及所携带的广谱抗生素及消毒剂耐药基因.方法 测定20株分离自烧伤患者的鲍曼不动杆菌对四环素、米诺环素、氯霉素、利福平、复方磺胺甲噁唑5种广谱抗生素的敏感性,PCR检测catB、cmlA、arr-2/3、tetA、tetB、smr-2、emrE、dfrA1、dfrA5、dfrA7、dfrA12、dfrA17、dfrB5、qacE△l-sull和intI 共15种基因.结果 20株细菌对5种抗生素的敏感率分别为10%、100%、0、0和5%.tetB、qacE△l-sull和intl基因检出率均为95%(19/20),其余12种基因为阴性,且一株静脉导管分离株携带了上述3种基因.结论 本组鲍曼不动杆菌烧伤分离株对除米诺环素外的广谱抗生素耐药严重,并携带了四环素类和消毒剂耐药基因.应规范此类抗生素在养殖业中的使用,同时采取措施防止多重耐药菌株利用静脉导管在烧伤科传播.%Objective To investigate the broad-spectrum antibiotics resistance and broad-spectrum antibiotics and antiseptics resistance genes of Acinetobacter baumannii strains isolated from burned patients.Methods Susceptibilities to tetracycline, minocycline, chloramphenicol, rifampicin and trimethoprim-sulfamethoxazole were tested.Subsequently, catB, cmlA, arr-2/3, tetA, tetB, smr-2, emrE, dfrA1, dfrA5, dfr4 7, dfr412, dfrA17, dfrB5, qacE⊿l-sull and intI were detected by PCR.Results Susceptibilities to five broad-spectrum antibiotics were 10%, 100%, 0, 0 and 5% respectively.19/20(95%) isolates carried tetB, qacE⊿l-sull and intI genes while other 12 genes were not detected.Notably, an isolate colonising a central venous catheter carried all three genes mentioned above.Conclusions Acinetobacter baumannii strains isolated from burned patients we studied had serious resistances to broad-spectrum antibiotics except minocycline and carried tetracyclines and antiseptics resistance genes.Accordingly, broad-spectrum antibiotics should be

  16. Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers Evaluación microbiológica de las diferentes formulaciones antisépticas, polivinil-pirrolidona-yodo y clorexidina, después de la contaminación intencional de los recipientes Avaliação microbiológica das diferentes formulações anti-sépticas, polivinilpirrolidona-iodo e clorexidina, após contaminação intencional das almotolias

    Directory of Open Access Journals (Sweden)

    Christiane Moreira Padovani

    2008-12-01

    Full Text Available This study aimed to evaluate the survival rate of microorganisms within different antiseptic formulations - povidone-iodine (PVP-I and chlorhexidine (CHX - after intentional contamination, and to establish the minimum care necessary to ensure sterilization of non-disposable antiseptic solution containers. A laboratory study was performed with 180 antiseptic containers, which were contaminated with Serratia marcescens [1 x 105 UFC/mL]. The containers were closed and stored, at room temperature, during seven days and shaken daily. The antiseptic cultures were evaluated to be 100% negative to Serratia marcescens in all of the non-disposable containers. These results suggested that antiseptic solutions inactivate microorganisms [1 x 105 UFC/mL]. Since cleaned antiseptic containers have around 102 UFC coming from tap water, it can be inferred that cleansing is a safe minimum procedure to ensure reuse of containers for distribution of CHX and PVP-I solutions in aqueous, detergent and alcoholic formulations.Los objetivos de este estudio fueron evaluar la sobre vivencia de los micro organismos en las diferentes formulaciones de los antisépticos clorexidina (CHX y polivinil-pirrolidona-yodo (PVP-Y, después de una contaminación intencional de los recipientes y extrapolar los resultados de los laboratorios para el cuidado mínimo a ser dispensado a los recipientes de múltiple uso para el envase de los antisépticos probados. Para esto, fue desarrollado un estudio de laboratorio, en que 180 recipientes fueron contaminados con 1 x 105 UFC/mL de suspensión conteniendo S.marcescens. Después de la contaminación, seis diferentes formulaciones de antisépticos (clorexidina y PVP-Y en los vehículos alcohólico, detergente y acuoso fueron distribuidos y sometidos a la cultura diaria durante siete días, a fin de verificar se hubo crecimiento del microorganismo. Los resultados de esta investigación permiten recomendar la limpieza como el procedimiento m

  17. [Antimicrobial properties of antiseptic composite with prolonged action].

    Science.gov (United States)

    Paliĭ, G K; Nazarchuk, A A; Paliĭ, D V; Nazarchuk, G G; Gonchar, O O; Sukhliak, V V; Trofimenko, Iu Iu; Zadereĭ, N V; Stukan, O K

    2013-01-01

    Antimicrobial properties of a composite based on decamethoxine and modified polysaccharides (carboxymethylamylum, oxyethyl-cellulose) were studied. The composite was shown to have high antimicrobial activity against grampositive and gramnegative bacteria under different conditions of the experiment.

  18. 抗菌内外墙涂料及抗菌腻子%Antiseptic Coating and Antiseptic Luting

    Institute of Scientific and Technical Information of China (English)

    田树霖; 康平

    2003-01-01

    @@涂料是建筑装饰行业不可缺少的原材料。随着人民生活水平提高,涂料产量逐年猛增,1996年高达150万吨,并以每年10%速度增长,截止2002年8月统计,我国年产各种涂料约200万吨。其中以有机溶剂为分散介质的毒性强、污染严重的涂料虽逐渐被淘汰,但目前……

  19. Pesquisa de Micobactérias Ambientais em água de torneira, luvas e soluções utilizadas em procedimentos cirúrgicos no Hospital Universitário Getúlio Vargas - Manaus/AM Investigation of Environmental Mycobacteria in tap water, surgical gloves and antiseptic solutions used in surgical procedures at the Getúlio Vargas University Hospital, Manaus-AM/Brazil

    Directory of Open Access Journals (Sweden)

    Adriana Vallejo Restrepo

    2009-01-01

    Full Text Available Investigou-se por métodos bacteriológicos (cultivo e moleculares (PCR - Restriction Enzyme Analysis, PRA, a presença de micobactérias ambientais em águas de torneira, soluções e luvas cirúrgicas, utilizadas nas etapas dos procedimentos cirúrgicos executados no centro cirúrgico do Hospital Universitário Getulio Vargas (HUGV, na cidade de Manaus-Amazonas/Brasil. Foram colhidas e analisadas 105 amostras sendo: 24 de águas (colhidas das 2 torneiras existentes no centro cirúrgico, 8 de solução de Povidine e 7 de solução de Clorhexidina, que servem para a higienização das mãos dos cirurgiões; 39 de luvas cirúrgicas (superfícies internas e externas; e 27 de soluções que foram efetivamente utilizadas durante o ato cirúrgico. Por método bacteriológico obteve-se 41 isolados micobacterianos apenas de águas das torneiras. Pelo PRA obteve-se a detecção de DNA micobacteriano somente na amostra de água que forneceu acima de 100 colônias de micobactérias por tubo semeado. Os isolados foram identificados como sendo Mycobacterium celatum perfil 2, M. gordonae perfil 3, M. gordonae perfil 6, M. intracellulare perfil 1, M. lentiflavum perfil 3 e M. mucogenicum perfil 1. O encontro de M. mucogenicum, espécie já incriminada em surtos pós-cirúrgicos, indica que devem ser efetuados procedimentos de limpeza e monitoramento em todos os pontos de distribuição de águas, visando à prevenção de surtos de micobacterioses nosocomiais induzidos pelo uso das águas nas diferentes atividades de manuseio ou higienização dos pacientes submetidos a procedimentos invasivos.Using bacteriological (culture and molecular (PCR - Restriction Enzyme Analysis, PRA methods, we investigated the presence of environmental mycobacteria in tap water, antiseptic solutions and surgical gloves, used in carrying out surgical procedures at the Getúlio Vargas University Hospital Surgical Center, in Manaus -Amazonas/Brazil. Samples (105 were collected and

  20. Percutaneous treatment of chronic MRSA osteomyelitis with a novel plant-derived antiseptic

    Directory of Open Access Journals (Sweden)

    Boeck Harry

    2001-05-01

    Full Text Available Abstract Background Antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA and vancomycin-resistant enterococcus (VRE, are an increasing problem world-wide, causing intractable wound infections. Complex phytochemical extracts such as tea tree oil and eucalypt-derived formulations have been shown to have strong bactericidal activity against MRSA in vitro. Polytoxinol (PT antimicrobial, is the trade name of a range of antimicrobial preparations in solution, ointment and cream form. Methods We report the first use of this drug, administered percutaneously, via calcium sulphate pellets (Osteoset,TM, into bone, to treat an intractable MRSA infection of the lower tibia in an adult male. Results and Discussion Over a three month period his symptoms resolved with a healing response on x-ray and with a reduced CRP.

  1. The efficacy of antiseptic mouth rinses in comparison with dental floss in controlling interproximal gingivitis

    OpenAIRE

    Mythri, H.; Ananda, S. R.; G M Prashant; V V Subba Reddy; Chandu, G. N.

    2011-01-01

    Objectives: Maintaining good oral hygiene is important to combat periodontal diseases. The use of tooth brush alone does not serve the purpose, especially in inaccessible areas like proximal embrasures, which demand the use of some adjuncts like proximal cleaning aids. Hence, the objective of this study was to compare the clinical efficacy of two antimicrobial mouth rinses (Cool mint Listerine and 0.2% Chlorhexidine gluconate) with dental floss in reducing interproximal gingivitis and dental ...

  2. First detection of the antiseptic resistance gene qacA/B in Enterococcus faecalis.

    Science.gov (United States)

    Bischoff, Meike; Bauer, Johann; Preikschat, Petra; Schwaiger, Karin; Mölle, Gabriele; Hölzel, Christina

    2012-02-01

    Resistance to disinfectants is well investigated in staphylococci and pseudomonads but nearly unexplored in bacteria of the genus Enterococcus, despite their rising significance as nosocomial pathogens. In this study, Enterococcus faecalis (n=585) from blood (n=42) and stool (n=109) of hospitalized humans, from faeces of farm animals (n=226), and from food (milk and dairy products, n=96; meat and meat products, n=112) were screened for the presence of qac-genes (qacA, qacB, qacC, smr [qacC+qacD], qacEΔ1, qacG, qacH, qacJ) via PCR. The isolates' susceptibility to a quaternary ammonium compound (didecyldimethylammoniumchloride, DDAC) and antibiotics was assessed by microdilution. Four E. faecalis strains were positive for qac-genes: qacA/B was found in one isolate from cattle and one isolate from human blood; smr (qacC+qacD) was detected in one isolate from human stool and in one isolate from cheese ("Camembert"). The sequences of the qacA/B-amplicons differed in two basepairs. DDAC had an elevated minimum inhibitory concentration (MIC) of 2.45-3.5 mg/L in one qacA/B-positive strain from human blood, whereas the other qac-gene carriers had wild-type MIC-values for DDAC (1.05 mg/L). This is the first detection of qacA/B in the genus Enterococcus.

  3. Antibacterial Chemical Constituent and Antiseptic Herbal Soap from Salvinia auriculata Aubl.

    OpenAIRE

    Samia Lima; Gaspar Diaz; Marisa Alves Nogueira Diaz

    2013-01-01

    The bioassay-guided isolation of the active extract of Salvinia auriculata Aubl. led to the separation of three main compounds, characterized as stigmasterone, stigmasterol, and friedelinol. The pure form of diketosteroid presented a potential antibacterial activity with a minimum inhibitory concentration (MIC) value of 0.01 mg mL−1 against Staphylococcus aureus isolated from animals with mastitis infections. The active extract also showed a similar result to that previously obtained with pur...

  4. Antibacterial Chemical Constituent and Antiseptic Herbal Soap from Salvinia auriculata Aubl.

    Science.gov (United States)

    Lima, Samia; Diaz, Gaspar; Diaz, Marisa Alves Nogueira

    2013-01-01

    The bioassay-guided isolation of the active extract of Salvinia auriculata Aubl. led to the separation of three main compounds, characterized as stigmasterone, stigmasterol, and friedelinol. The pure form of diketosteroid presented a potential antibacterial activity with a minimum inhibitory concentration (MIC) value of 0.01 mg mL(-1) against Staphylococcus aureus isolated from animals with mastitis infections. The active extract also showed a similar result to that previously obtained with pure diketosteroid when tested with the same isolates. The present study's results demonstrate the potential of this plant as an excipient for the production of antibacterial soaps aimed at controlling bovine mastitis infections, especially on small farms. PMID:24459530

  5. Antibacterial Chemical Constituent and Antiseptic Herbal Soap from Salvinia auriculata Aubl.

    Directory of Open Access Journals (Sweden)

    Samia Lima

    2013-01-01

    Full Text Available The bioassay-guided isolation of the active extract of Salvinia auriculata Aubl. led to the separation of three main compounds, characterized as stigmasterone, stigmasterol, and friedelinol. The pure form of diketosteroid presented a potential antibacterial activity with a minimum inhibitory concentration (MIC value of 0.01 mg mL−1 against Staphylococcus aureus isolated from animals with mastitis infections. The active extract also showed a similar result to that previously obtained with pure diketosteroid when tested with the same isolates. The present study’s results demonstrate the potential of this plant as an excipient for the production of antibacterial soaps aimed at controlling bovine mastitis infections, especially on small farms.

  6. Chlorhexidine-based antiseptic solutions effectively reduce catheter-related bacteremia.

    Science.gov (United States)

    Onder, Ali Mirza; Chandar, Jayanthi; Billings, Anthony; Diaz, Rosa; Francoeur, Denise; Abitbol, Carolyn; Zilleruelo, Gaston

    2009-09-01

    The aim of this retrospective study was to investigate if the application of chlorhexidine-based solutions (ChloraPrep) to the exit site and the hub of long-term hemodialysis catheters could prevent catheter-related bacteremia (CRB) and prolong catheter survival when compared with povidone-iodine solutions. There were 20,784 catheter days observed. Povidone-iodine solutions (Betadine) were used in the first half of the study and ChloraPrep was used in the second half for all the patients. Both groups received chlorhexidine-impregnated dressings at the exit sites. The use of ChloraPrep significantly decreased the incidence of CRB (1.0 vs 2.2/1,000 catheter days, respectively, P = 0.0415), and hospitalization due to CRB (1.8 days vs 4.1 days/1,000 catheter days, respectively, P = 0.0416). The incidence of exit site infection was similar for the two groups. Both the period of overall catheter survival (207.6 days vs 161.1 days, P = 0.0535) and that of infection-free catheter survival (122.0 days vs 106.9 days, P = 0.1100) tended to be longer for the catheters cleansed with ChloraPrep, with no statistical significance. In conclusion, chlorhexidine-based solutions are more effective for the prevention of CRB than povidone-iodine solutions. This positive impact cannot be explained by decreased number of exit site infections. This study supports the notion that the catheter hub is the entry site for CRB. PMID:19296135

  7. Photocytotoxicity of a 5-nitrofuran-ethenyl-quinoline antiseptic (Quinifuryl to P388 mouse leukemia cells

    Directory of Open Access Journals (Sweden)

    Daghastanli N.A.

    2004-01-01

    Full Text Available Quinifuryl (MW 449.52, 2-(5'-nitro-2'-furanylethenyl-4-{N-[4'-(N,N-diethylamino-1'-methylbutyl]carbamoyl} quinoline, is a water soluble representative of a family of 5-nitrofuran-ethenyl-quinoline drugs which has been shown to be highly toxic to various lines of transformed cells in the dark. In the present study, the toxicity of Quinifuryl to P388 mouse leukemia cells was compared in the dark and under illumination with visible light (390-500 nm. Illumination of water solutions of Quinifuryl (at concentrations ranging from 0.09 to 9.0 µg/ml in the presence of P388 cells resulted in its photodecomposition and was accompanied by elevated cytotoxicity. A significant capacity to kill P388 cells was detected at a drug concentration as low as 0.09 µg/ml. The toxic effect detected at this drug concentration under illumination exceeded the effect observed in the dark by more than three times. Moreover, the general toxic effect of Quinifuryl, which included cell proliferation arrest, was nearly 100%. Both dose- and time-dependent toxic effects were measured under illumination. The LC50 value of Quinifuryl during incubation with P388 cells was ~0.45 µg/ml under illumination for 60 min and >12 µg/ml in the dark. We have demonstrated that the final products of the Quinifuryl photolysis are not toxic, which means that the short-lived intermediates of Quinifuryl photodecomposition are responsible for the phototoxicity of this compound. The data obtained in the present study are the first to indicate photocytotoxicity of a nitroheterocyclic compound and demonstrate the possibility of its application as a photosensitizer drug for photochemotherapy.

  8. 水下防腐混凝土的配制%Distribution of underwater antiseptic concrete

    Institute of Scientific and Technical Information of China (English)

    张俊岭

    2005-01-01

    结合具体的工程实践,从原材料选择、配合比设计、计算防腐混凝土配比等方面,阐述了防腐混凝土的配制,并对水下防腐混凝土灌注效果作了分析,指出其经济效益明显.

  9. Organ mercury levels in infants with omphaloceles treated with organic mercurial antiseptic

    Energy Technology Data Exchange (ETDEWEB)

    Fagan, D.G.; Pritchard, J.S.; Clarkson, T.W.; Greenwood, M.R.

    1977-01-01

    Samples of fresh and fixed tissues from infants with exomphalos treated by thiomersal application were analyzed for mercury content. The results showed that thiomersal can induce blood and organ levels of organic mercury which are well in excess of the minimum toxic level in adults and fetuses. The analysis of fresh and fixed tissues must be carefully controlled against normal tissues in order to interpret mercury levels accurately.

  10. Septisol antiseptic foam: a sensible alternative to the conventional surgical scrub.

    Science.gov (United States)

    Rubio, P A

    1987-01-01

    Over a recent 8-year period, Septisol Foam (0.23% hexachlorophene in a 46% ethyl alcohol base) alone was used to prepare the hands and forearms of the author's surgical team. In 3480 cases, only 11 postoperative infections were encountered, for an incidence of 0.3%. All 11 infections involved patients who underwent gastrointestinal surgery and/or who had high-risk factors such as cancer, hypertension, diabetes mellitus, perforated appendix, emergency surgery, age over 65 years, and foreign bodies in the form of hemodialysis access shunts. Septisol Foam is an effective bacteriostatic agent that minimizes the risk of dermatitis and offers sufficient antibacterial protection without supplementary handwashing. When used according to the method described here, the foam eliminates the need for scrub brushes and reduces the total scrub time to approximately 2 minutes in each case. In addition to being safe, effective, and easy to use, this method results in a marked decrease in water consumption and considerable monetary savings.

  11. 77 FR 69863 - Antiseptic Patient Preoperative Skin Preparation Products; Public Hearing; Request for Comments

    Science.gov (United States)

    2012-11-21

    ... purposes in healthcare or home settings (e.g., wound care or maintenance care for indwelling catheters)? If..., healthcare personnel handwash, or skin wound and general skin cleanser. Despite their inherent...

  12. The Soluble and Antiseptic Effects of 5 Different Antiseptic Agents in the Solution of 50% Magnesium Sulfate%五种防腐剂在50%硫酸镁溶液中的溶解及防腐效果

    Institute of Scientific and Technical Information of China (English)

    莫玉芳; 叶延武

    2006-01-01

    目的筛选50%硫酸镁溶液的防腐剂.方法比较五种防腐剂在50%硫酸镁溶液的溶解情况以及防腐效果.结果在抑菌浓度范围内,度灭芬在50%硫酸镁溶液中溶解完全,防腐效果佳,苯甲酸次之.结论度灭芬作为50%硫酸镁溶液的防腐剂,效果最好.

  13. Sustained ex vivo skin antiseptic activity of chlorhexidine in poly(epsilon-caprolactone) nanocapsule encapsulated form and as a digluconate.

    Science.gov (United States)

    Lboutounne, Hassan; Chaulet, Jean-François; Ploton, Christine; Falson, Françoise; Pirot, Fabrice

    2002-08-21

    In this work, the sustained bactericidal activity of chlorhexidine base loaded poly(epsilon-caprolactone), PCL, nanocapsules against Staphylococcus epidermidis inoculated onto porcine ear skin was investigated. Drug loaded nanocapsules were prepared by the interfacial polymer deposition following solvent displacement method, then characterized by photon correlation spectroscopy, electrophoretic measurements, transmission and scanning electron microscopy. Antimicrobial activity of these colloidal carriers was evaluated (i) in vitro against eight strains of bacteria, and (ii) ex vivo against Staphylococcus epidermidis inoculated for 12 h onto porcine ear skin surface treated for 3 min either with 0.6% chlorhexidine base loaded or unloaded nanocapsules suspended in hydrogel, or 1% chlorhexidine digluconate aqueous solution. Chlorhexidine absorption into the stratum corneum (SC) was evaluated by the tape-stripping method. The results showed that chlorhexidine nanocapsules in aqueous suspension having a 200-300 nm size and a positive charge exhibited similar minimum inhibitory concentrations against several bacteria with chlorhexidine digluconate aqueous solution. Ex vivo, there was a significant reduction in the number of colony forming units (CFUs) from 3-min treated skin with chlorhexidine nanocapsule suspension (5 to <1 log(10)) compared to chlorhexidine digluconate solution (5 to 2.02 log(10)) after a 8-h artificial contamination. After a 12-h artificial contamination, both formulations failed to achieve a 5 log(10) reduction. Furthermore, from a 3-min treatment with an identical applied dose and a subsequent 12-h artificial contamination, a residual chlorhexidine concentration in the SC was found to be three-fold higher with chlorhexidine nanocapsule suspension than with chlorhexidine digluconate solution. Interestingly, nanocapsules were shown in porcine skin follicles. Consequently, a topical application of chlorhexidine base-loaded positively charged nanocapsules in an aqueous gel achieved a sustained release of bactericide against Staphylococcus epidermidis for at least 8 h. Enhancement of drug delivery by mediating a more direct and prolonged contact between the carrier and (i) bacteria, (ii) skin surface, and (iii) skin follicles was assumed.

  14. 橡胶木的防腐及干燥%Antiseptic treating and drying for Hevea wood

    Institute of Scientific and Technical Information of China (English)

    黄荣文

    2002-01-01

    橡胶木作为商品材具有双重经济价值,在木材利用方面因其易受真菌和昆虫的侵蚀,妨碍了对其材质的使用。现从木材干燥的角度论述:如何最大限度地减少真菌的产生和昆虫的侵蚀,合理地利用橡胶木。

  15. Behaviour of antiseptic containing chlorine and its application%含氯消毒剂的性能与应用

    Institute of Scientific and Technical Information of China (English)

    曲显恩

    2005-01-01

    详细介绍了含氯消毒剂的组成,性能及应用范围.主要成分次氯酸钠的化学特性、杀菌作用和杀菌机理.阐述了基于次氯酸钠的不稳定性和腐蚀性,提出了解决措施.

  16. [Comparative study of the effects of a local antibiotic and a local antiseptic in emergency hand surgery].

    Science.gov (United States)

    Iselin, F; Audren, J L; Gouet, O; Hautefort, E; Peze, W; Pradet, G

    1990-01-01

    An open randomised comparative study was conducted in patients presenting with a hand injury requiring a surgical operation, in order to compare the efficacy of topical application of rifamycin SV with that of iodinated polyvidone dermal solution, in terms of the quality and rate of healing evaluated by the clinician, 268 patients were included in the study and 223 of them participated in the analysis of the results. Analysis of the results demonstrated the following conclusions: signs of infection developed in 8 patients in the rifamycin SV group (7%) and in 20 patients in the iodinated polyvidone group (18.5%). This difference was significant (p = 0.011) in favour of rifamycin SV. The rate of healing was considered to be rapid in 10% of patients in the rifamycin SV group and in 4% of patients in the iodinated polyvidone group. It was considered to be slow in 14% of the subjects in the rifamycin SV group and in 21% of those in the iodinated polyvidone group. This difference was also significant in favour of the rifamycin SV group (p = 0.038). In terms of local tolerance, 32 patients equally distributed between the two treatment groups developed signs of cutaneous intolerance.

  17. Development of an antiseptic procedure to improve retention rates during the grafting process of black-lip oyster, Pinctada margaritifera

    OpenAIRE

    Cochennec, Nathalie; Haffner, Philippe; Levy, Peva; Saulnier, Denis; Fougerouse, A; Langy, Sandra

    2006-01-01

    In French Polynesia, black pearl cultivation now takes place on about 30 of the 118 atolls or islands. This activity has largely replaced copra, coconut oil and fishing as a source of income. Around 1,000 pearl farms, generally small ones of less than 5 ha, are registered. The pearl farms are mainly owned by families. In 1998, about 7,000 persons were employed in theentire pearl industry, from production to retailing. In value, this activity represents the first exportation of French Po...

  18. Microbiological and biochemical effectiveness of an antiseptic gel on the bacterial contamination of the inner space of dental implants: a 3-month human longitudinal study.

    Science.gov (United States)

    D'Ercole, S; Tetè, S; Catamo, G; Sammartino, G; Femminella, B; Tripodi, D; Spoto, G; Paolantonio, M

    2009-01-01

    Microbial penetration inside the implants internal cavity produces a bacterial reservoir that is associated with an area of inflamed connective tissue facing the fixture-abutment junction. The aim of this clinical trial is to evaluate the effectiveness of a 1 percent chlorhexidine gel on the internal bacterial contamination of implants with screw-retained abutments and on the level of AST secreted in peri-implant crevicular fluid. Twenty-five patients (aged 29 to 58 years) each received one implant. Three months after the end of the restorative treatment, and immediately after a clinical and radiographic examination and the abutment removal, microbiological samples were obtained from the internal part of each fixture and biochemical samples were collected by peri-implant sulci. The patients were then divided into two groups: the control (CG; n=10) and test (TG; n=15) groups. The CG had the abutment screwed into place and the crown cemented without any further intervention. In contrast, before the abutment placement and screw tightening, the TG had the internal part of the fixture filled with a 1 percent chlorhexidine gel. Three months later, the same clinical, microbiological and biochemical procedures were repeated in both groups. Total bacterial count, specific pathogens and AST activity were detected. The clinical parameters remained stable throughout the study. From baseline to the 3-month examination, the total bacterial counts underwent a significant reduction only in the TG. In contrast, the AST activity showed a significant increase in the CG. The administration of a 1% chlorhexidine gel appears to be an effective method for the reduction of bacterial colonization of the implant cavity and for safeguarding the health status of peri-implant tissue over a 3-month administration period.

  19. Synthesis of Highly Active Rare Earth Cerium/Silver Mesoporous Composite Antiseptics%高活性铈银介孔复合抗菌剂的制备

    Institute of Scientific and Technical Information of China (English)

    陆斌; 李辉; 张昉; 张蝶青; 何其庄; 李和兴

    2008-01-01

    利用分步浸渍法制备了Ce银介孔复合无机抗菌剂,并对其进行XRD,TEM,N2吸脱附等表征,结果显示,该抗菌剂仍然保持有序介孔结构,活性物种Ag以纳米线状稳定存在于孔道内.运用抑菌圈法和最小抑菌浓度法对其抗菌性能进行了评价,测试表明,该样品对金黄色葡萄球菌和大肠杆菌均有着优良的抑杀效果.这是由于抗菌活性物种Ag高度分散在介孔材料孔道中,以及Ce有促进Ag+产生羟基自由基,因而能强化Ag的抗菌性能的原因.

  20. Amuchina 10% solution, safe antiseptic for preventing infections of exit-site of Tenckhoff catheters, in the pediatric population of a dialysis program.

    Science.gov (United States)

    Mendoza-Guevara, L; Castro-Vazquez, F; Aguilar-Kitsu, A; Morales-Nava, A; Rodriguez-Leyva, F; Sanchez-Barbosa, J L

    2007-01-01

    Although, decreasing in incidence with the disconnection systems, the first complication is still peritonitis in patients with chronic renal failure and the second is infection of Tenckhoff catheter exit-site. All efforts made to diminish the frequency of exit-site infection lower the possibility of peritonitis. The pediatric population is well-known to have a major risk of infectious complications, and making easy and safe the care of the exit-site will prevent the peritonitis that follows. The aim of the study was to evaluate the efficacy of the Amuchina 10% solution vs. pH neutral soap in children with chronic renal failure, on preventing exit-site infection. There were 60 patients who were assigned randomly to one of two groups. One group used Amuchina 10% solution for the daily cleaning of the exit-site, and the other used pH neutral soap, with 14 months of follow-up. Before the study they have to be free of infection for at least 30 days. All were taught by the same nurse how to clean their exit-site. Groups were almost identical in years, sex, and time on dialysis. We had nine infections in the soap group and none in the Amuchina 10% solution group, with an OR: 17 (p = 0.004). From these nine infections, the bacteria isolated were: 4 (13%) were caused by Pseudomona aeruginosa, 1 (3.3%) by Staphylococcus aureus, coagulase-positive staphylococci in 2 (6.6%) and Serratia marcensens in 1 (3.3%). In conclusion, Amuchina 10% solution is effective in preventing infection on the exit-site, without any secondary topical reaction.

  1. Application of antiseptic napkins on patients with tracheotomy%灭菌餐巾纸在气管切开患者中的应用

    Institute of Scientific and Technical Information of China (English)

    徐敏; 忻珊

    2007-01-01

    气管切开是临床上抢救呼吸困难及呼吸衰竭患者的一种重要而积极的措施。为了预防切口和肺部感染,常规在气管套管口垫气管切开纱布,一般每天更换1~2次,但对于痰液较多并能自行咳出的患者,更换频率就要增加。2005年1月至2005年9月,本院神经内科收治的气管切开患者中,有11例气管切开纱布的更换频率平均在1h左右更换1次,频繁地更换纱布易引起气管套管脱出,增加患者痛苦和经济负担。为减少气管切开纱布的更换次数,从2005年10月起,在气管切开纱布上加垫1张灭菌餐巾纸,效果较好。现介绍如下。

  2. Research and producing of SX efficient antiseptic napkin%SX高效杀菌消毒餐巾纸的研制

    Institute of Scientific and Technical Information of China (English)

    马铭杰; 崔中敏; 张彦浩

    2006-01-01

    研制一种以漂粉精为主原料加入适量助剂,能够快速杀菌消毒的实用餐巾纸,实验证明该餐巾纸对细菌、乙肝病毒具有强力杀灭作用和良好的贮存稳定性,成功地解决了含有效氯类消毒液贮存中易分解难题.

  3. Amuchina 10% solution, safe antiseptic for preventing infections of exit-site of Tenckhoff catheters, in the pediatric population of a dialysis program.

    Science.gov (United States)

    Mendoza-Guevara, L; Castro-Vazquez, F; Aguilar-Kitsu, A; Morales-Nava, A; Rodriguez-Leyva, F; Sanchez-Barbosa, J L

    2007-01-01

    Although, decreasing in incidence with the disconnection systems, the first complication is still peritonitis in patients with chronic renal failure and the second is infection of Tenckhoff catheter exit-site. All efforts made to diminish the frequency of exit-site infection lower the possibility of peritonitis. The pediatric population is well-known to have a major risk of infectious complications, and making easy and safe the care of the exit-site will prevent the peritonitis that follows. The aim of the study was to evaluate the efficacy of the Amuchina 10% solution vs. pH neutral soap in children with chronic renal failure, on preventing exit-site infection. There were 60 patients who were assigned randomly to one of two groups. One group used Amuchina 10% solution for the daily cleaning of the exit-site, and the other used pH neutral soap, with 14 months of follow-up. Before the study they have to be free of infection for at least 30 days. All were taught by the same nurse how to clean their exit-site. Groups were almost identical in years, sex, and time on dialysis. We had nine infections in the soap group and none in the Amuchina 10% solution group, with an OR: 17 (p = 0.004). From these nine infections, the bacteria isolated were: 4 (13%) were caused by Pseudomona aeruginosa, 1 (3.3%) by Staphylococcus aureus, coagulase-positive staphylococci in 2 (6.6%) and Serratia marcensens in 1 (3.3%). In conclusion, Amuchina 10% solution is effective in preventing infection on the exit-site, without any secondary topical reaction. PMID:17099309

  4. Antiseptic efficacy of selected agents and tissue tolerable plasma (TTP) on C. albicans biofilms – has the biofilm maturity influence on it?

    OpenAIRE

    Koban, Ina; Hübner, Nils-Olaf; Matthes, Rutger; Welk, Alexander; Kindel, Eckhard; Weltmann, Klaus-Dieter; Kramer, Axel; Kocher, Thomas

    2009-01-01

    Background: The formation of biofilms is crucial to the pathogenesis of many dental microbial infections. Oral candidosis are common and often found under removable partial dentures. Nonthermal atmospheric plasma (tissue tolerable plasma, TTP) was tested for its antimicrobial activity against different matured Candida albicans biofilms.Methods: We assessed the efficacy of selected agents (chlorhexidine, sodium hypochlorite, fluconazol) and TTP against in vitro biofilms of C. albicans grown 12...

  5. Contrast of bacteriostasis effect between aloe and common food antiseptic%芦荟与常见食品防腐剂的抑菌效果比较

    Institute of Scientific and Technical Information of China (English)

    陈涛

    2009-01-01

    以中华芦荟为研究对象,比较了芦荟和食品防腐剂的抑菌作用.结果表明,高浓度的芦荟汁具有一定的抑菌能力,其抑菌效果与被测试防腐剂的抑菌效果相近.

  6. Stability and molecular dynamics of chloroxylenol (API of antiseptics and drugs) in solid state studied by 35Cl-NQR spectroscopy and DFT calculations

    Science.gov (United States)

    Latosińska, J. N.; Tomczak, M. A.; Kasprzak, J.

    2009-02-01

    Thermal stability of 4-chloro-3,5-dimethyl-phenol (chloroxylenol) in solid state has been studied by 35Cl-NQR spectroscopy. Two NQR resonance lines at the frequencies 34.348 and 34.415 MHz at 77 K have been assigned to chlorine atoms from two crystallographically inequivalent molecules on the basis of the B3LYP/6-311++G∗∗ results. The temperature dependence of the resonance frequency and full width at half maximum suggest the occurrence of small-angle torsional oscillations of the mean activation energy of 3.83 kJ/mol and rotation of both methyl groups around their symmetry axis C3 with the activation energies 12.49 and 11.27 kJ/mol for CH3 in molecule A and B, respectively. B3LYP/6-311++G∗∗ method reproduced very well the activation energies of both motions.

  7. Drug: D00008 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available A01 STOMATOLOGICAL PREPARATIONS A01A STOMATOLOGICAL PREPARATIONS A01AB Antiinfectives and antiseptics for lo...ide (USP) D DERMATOLOGICALS D08 ANTISEPTICS AND DISINFECTANTS D08A ANTISEPTICS AND DISINFECTANTS D08AX Other antiseptic

  8. Drug: D01711 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available iseptics and disinfectants D08AX07 Sodium hypochlorite D...C) classification [BR:br08303] D DERMATOLOGICALS D08 ANTISEPTICS AND DISINFECTANTS D08A ANTISEPTICS AND DISINFECTANTS D08AX Other ant

  9. White blood cell count - series (image)

    Science.gov (United States)

    ... the hand. The puncture site is cleaned with antiseptic, and a tourniquet (an elastic band) or blood ... or young child: The area is cleansed with antiseptic and punctured with a sharp needle or a ...

  10. 21 CFR 347.20 - Permitted combinations of active ingredients.

    Science.gov (United States)

    2010-04-01

    ... antiseptic active ingredients. Any one (two when required to be in combination) or more of the active... generally recognized as safe and effective single first aid antiseptic active ingredient, or any...

  11. 21 CFR 347.60 - Labeling of permitted combinations of active ingredients.

    Science.gov (United States)

    2010-04-01

    ... labeled for concurrent symptoms. (2) Combinations of skin protectant and first aid antiseptic active... § 347.50(b)(1), the required indications for first aid antiseptic drug products should be used....

  12. Antiseptica ter preventie of behandeling van wondinfecties

    NARCIS (Netherlands)

    Ubbink, D.T.; Vermeulen, H.; Lubbers, M.J.; Voss, A.

    2009-01-01

    Antiseptics are commonly applied to prevent or treat wound infections. Many common opinions about antiseptics appear not to be supported by convincing evidence. The aim of this review is to compare such traditional beliefs with currently available evidence. While antiseptics obviously reduce the num

  13. 30 CFR 50.20-3 - Criteria-Differences between medical treatment and first aid.

    Science.gov (United States)

    2010-07-01

    ..., first degree burns and splinters. Ointments, salves, antiseptics, and dressings to minor injuries are..., applying antiseptic and nonprescription medication and bandages on the first visit and follow-up visits... antiseptic constitutes first aid where it is required by work duties that soil the bandage. (ii)...

  14. Studies on Hawthorn (Crataegus L.) Drink and Antiseptic Effect of Garlic (Allium sativum L.)%山楂(Crataegus L.)饮料及大蒜(Allium sativum L.)防腐效果研究

    Institute of Scientific and Technical Information of China (English)

    田学军; 郭亚力; 杨金; 闵勇

    2004-01-01

    以山楂叶、山楂果、蜂蜜、白糖等为原料,用大蒜作为天然防腐剂,研制含天然植物黄酮的健康饮料.通过3×3正交试验确定产品的最佳配方.结果表明,以5%山楂叶汁、50%山楂果汁、8%蜂蜜配制的饮料口感和理化性质较好,并含有保健黄酮;大蒜能有效地防止饮料腐败,有很好的防腐效果.

  15. Novel antiseptic compound OPB-2045G shows potent bactericidal activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus both in vitro and in vivo: a pilot study in animals.

    Science.gov (United States)

    Inoue, Yasuhide; Hagi, Akifumi; Nii, Takuya; Tsubotani, Yoshie; Nakata, Hikaru; Iwata, Koushi

    2015-01-01

    There is a need for new compounds to effectively treat methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). The novel monobiguanide compound 1-(3,4-dichlorobenzyl)-5-octylbiguanide gluconate (OPB-2045G) has potential bactericidal activity. We sought to elucidate the potency of OPB-2045G bactericidal activity against MRSA and VRE compared to those of chlorhexidine digluconate (CHG) and povidone iodine (PVP-I). In vitro bactericidal activity was analysed using minimum bactericidal concentration (MBC) as the index. The in vivo bactericidal efficacy of OPB-2045G was examined by determining MRSA and VRE contamination of the normal dorsal skin of mice following removal of hair. After a 3 min treatment period, the MBC of OPB-2045G was lower than that of CHG and PVP-I against standard strains and clinical isolates. Additionally, in our in vivo mouse model, the in vivo bactericidal activity of 1.5 % OPB-2045G (a clinically relevant dose) was higher than that of 0.5 % CHG and equivalent to that of 10 % PVP-I against MRSA. Similarly, the in vivo bactericidal activity of OPB-2045G was higher than that of 0.5 % CHG and 10 % PVP-I against VRE. OPB-2045G showed more potent bactericidal activity against MRSA and VRE both in vitro and in vivo compared to CHG and PVP-I, indicating that OPB-2045G may provide better protection against health care-associated infections caused by these pathogens.

  16. 肉桂、丁香、连翘挥发油联合抑菌作用研究%Study on Combined Antiseptic Effects of Cinnamon, Cloves and Forsythia Suspense Volatile Oils

    Institute of Scientific and Technical Information of China (English)

    黄涵年; 阙斐

    2016-01-01

    Spices volatile oils of cinnamon, cloves and forsythia suspense were extracted by steam distillation. The inhibition zone diameters, minimum inhibition concentrations ( MICs) and the antimicrobial activities of two volatile oils compound on Escherichia coli and Staphylococcus aureus were determined and evaluated. The results showed that the tested microorganisms were highly sensitive to three kinds of volatile oils, all with a dose effect. The antimicrobial activities were decreased with the decrease of concentration of the volatile oils. The combined use of cinnamon (2 times efficiency) and cloves volatile oils ( 2 times efficiency ) , cinnamon ( 2 times efficiency ) and forsythia suspense ( 4 times efficiency ) volatile oils both had additive effects on Escherichia coli, and synergic effects on Staphylococcus aureus ( all 4 times efficiency) , and the combined use of cloves and forsythia suspense volatile oils showed indifferent effects on Escherichia coli and Staphylococcus aureus.%水蒸气蒸馏提取肉桂、丁香、连翘三种香辛料挥发油,测定与评价三种挥发油对大肠杆菌与金黄色葡萄球菌的抑菌圈直径、最低抑菌浓度( MIC)以及挥发油两两复配联用的抑菌活性。结果显示,供试菌对三种挥发油均高度敏感,且存在剂量效应,随着挥发油浓度的降低而降低。另外,肉桂(增效2倍)和丁香(增效2倍)、肉桂(增效2倍)和连翘(增效4倍)挥发油复配后对大肠杆菌表现出相加作用,对金黄色葡萄球菌表现出协同作用(均增效4倍);而丁香和连翘挥发油复配后对大肠杆菌和金黄色葡萄球菌,则表现出无关作用。

  17. Study on the anti-pathogenic activities of capsaicin and effects of its antiseptic application to ketchup%辣椒碱抗病原菌活性及其在番茄酱防腐中的应用

    Institute of Scientific and Technical Information of China (English)

    郭丽; 王巧珍; 朱林

    2006-01-01

    从红辣椒中提取的辣椒碱对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌这3种常见的病原细菌及黑曲霉、啤酒酵母等7种病原真菌进行拮抗试验.结果表明,辣椒碱具有广谱的抗菌范围和较强的拮抗病原菌的活性,且抗菌活性有明显的量效关系,其中辣椒碱对供试细菌的拮抗作用远大于对供试真菌的作用.将辣椒碱添加到番茄酱中进行防腐试验的结果表明,辣椒碱可有效的抑制微生物的生长,从而延长番茄酱的贮藏期.

  18. 新疆地区黄花蒿粗提物的体外抑菌效果观察%Effect on Antiseptic Activity of Extracts of Artem/s/a annua in the Xinjiang Region

    Institute of Scientific and Technical Information of China (English)

    刘亚丽; 剡根强; 王静梅

    2009-01-01

    采用乙醚、乙醇及水煎法提取黄花蒿的活性物质,以平板孔穴法测定不同提取液的抑菌效果,结果表明:3种提取液对单核增多性李氏杆菌、白色念珠菌、鸡源大肠杆菌牛源金黄色葡萄球菌均有不同程度的抑菌作用,其中以乙醚提取液的效果最好;黄花蒿茎叶的乙醚提取液对单核增多性李氏杆菌的抑制作用最强.

  19. Mensurating the Antiseptic Activity of Extracts from Artemisia annua L. to Mold with the Filter Paper Method%滤纸片法测定黄花蒿提取物对霉菌的抑制活性

    Institute of Scientific and Technical Information of China (English)

    张佳; 王莹; 张峰; 王付丽

    2009-01-01

    应用滤纸片法测定了黄花蒿对霉菌的抑制活性.结果表明,黄花蒿提取物浓度为50mg·mL-1时对霉菌具有较强的抑制活性,抑菌圈直径为10~15mm,其对霉菌的最小抑制浓度为9~12 mg·mL-1.

  20. 外排泵抑制剂小肽1号对抗菌药抗菌活性的影响%The Effects of Efflux Pump Inhibitor No. 1 Small Peptide on Antibacterial Activity of Antiseptic Drug

    Institute of Scientific and Technical Information of China (English)

    裴亚玲; 袁业友; 胡功政; 刘建华; 魏永俊

    2009-01-01

    为了观察外排泵抑制剂小肽1号对抗菌药抗菌活性的影响,本试验采用标准微量稀释法,测定了6类8种抗菌药单用和与小肽1号联用对12株临床分离鸡大肠杆菌的MIC值.结果表明,12株鸡大肠杆菌中有9株为产超广谱酶的多重耐药菌株,小肽1号(1:2)使恩诺沙星等8种药物的抗菌活性多数增强2倍.使氟苯尼考对A8、A15的抗菌活性增强了4倍,小肽1号(1:2或1:4)使恩诺沙星、甲替沙星、环丙沙星的抗菌活性增强2倍,使左旋氧氟沙星对A13的抗菌活性增强4倍.以上结果表明,细菌外排泵抑制剂-小肽1号对大多数药物的抗菌活性有一定的增强作用,产酶多重耐药的鸡大肠杆菌至少同时存在产ESBLs、外排泵两种耐药机制.

  1. Outbreaks where food workers have been implicated in the spread of foodborne disease. Part 11. Use of antiseptics and sanitizers in community settings and issues of hand hygiene compliance in health care and food industries.

    Science.gov (United States)

    Todd, Ewen C D; Greig, Judy D; Michaels, Barry S; Bartleson, Charles A; Smith, Debra; Holah, John

    2010-12-01

    Hand washing with soap is a practice that has long been recognized as a major barrier to the spread of disease in food production, preparation, and service and in health care settings, including hospitals, child care centers, and elder care facilities. Many of these settings present multiple opportunities for spread of pathogens within at-risk populations, and extra vigilance must be applied. Unfortunately, hand hygiene is not always carried out effectively, and both enteric and respiratory diseases are easily spread in these environments. Where water is limited or frequent hand hygiene is required on a daily basis, such as for many patients in hospitals and astronauts in space travel, instant sanitizers or sanitary wipes are thought to be an effective way of preventing contamination and spread of organisms among coworkers and others. Most concerns regarding compliance are associated with the health care field, but the food industry also must be considered. Specific reasons for not washing hands at appropriate times are laziness, time pressure, inadequate facilities and supplies, lack of accountability, and lack of involvement by companies, managers, and workers in supporting proper hand washing. To facilitate improvements in hand hygiene, measurement of compliant and noncompliant actions is necessary before implementing any procedural changes. Training alone is not sufficient for long-lasting improvement. Multiactivity strategies also must include modification of the organization culture to encourage safe hygienic practices, motivation of employees willing to use peer pressure on noncompliant coworkers, a reward and/or penalty system, and an operational design that facilitates regular hand hygiene. PMID:21219754

  2. Progress of the Research on the Fresh-keeping Effects of Nisin Complex Antiseptic in the Pasteurized Meat Products%Nisin复合防腐剂对低温肉制品保鲜效果的研究进展

    Institute of Scientific and Technical Information of China (English)

    赵晔; 汪敏

    2009-01-01

    乳酸链球菌素(Nisin)复合防腐剂作为高效、安全的防腐剂,在低温肉制品的保鲜上具有良好的效果.本论文综述了近几年国内外Nisin复合防腐剂在低温肉制品保鲜效果中的研究进展以及对其发展前景的展望.

  3. 呼吸机相关性肺炎环境致病菌的监测及消毒对策%MONITORING AND ANTISEPTIC POLICY ANALYSIS OF ENVIRONMENT PATHOGENIC BACTERIA WITH VENTILATOR-ASSOCIATED PNEUMONIA

    Institute of Scientific and Technical Information of China (English)

    乔宏; 邓小虹; 李宗麟; 王若东; 张俐; 王劲

    2011-01-01

    Objective To research species and source of environment pathogenic bacteria with ventilator - associated pneumonia (VAP) and discuss to disinfection measures.Methods Field sampling and detection were used to investgate the material and enviromental contact with patient used ventilator in respsratory word and lCU in Beijing Hospital.Results Bacterium were detected out from the table outside environment, air, operation nurse's hands, air route, operation panel,the outer line interfacing, a bottle of the inner surface wet, water with wet of breathing machine.But the same kind of bacteria is not detected from sputum culture of contemporaneous in - patients and samples of environment with disinfected breathing machine.Conclusion Ectogenous infection was not detected from environment with disinfected breathing machine through above analysis.Trial of the surface with the guide to recover breath was effective for specification for the cleaning and sanitizing and reducing infection of VAP from environment.%目的 研究呼吸机相关性肺炎外环境中致病菌种类及其来源,探讨呼吸机消毒措施.方法 通过现场调查和采样检测方法,对北京市部分医院呼吸科和重症监护病房使用呼吸机的患者接触的物品与环境进行了调查.结果 医院呼吸科和重症监护病房物体表面、室内空气、呼吸机操作面板、外管路内接口、湿化瓶内表面、湿化水、呼吸机整机气路以及病房护士手上均检出细菌阳性标本,但患者痰液标本与消毒后呼吸机上和环境物品上均未检出同源菌株.结论 医院呼吸科和重症监护病房患者痰标本未检出与消毒后呼吸机上同源菌株,提示医院所实施的呼吸机清洗消毒指南规范的方法有效.

  4. 用于管道防腐的中频炉谐波状况研究%Harmonics Status of High Power Intermediate Frequency Inductive Stove Used by West-to-east Gas Pipeline Antiseptics Process and Tackle Blue Print

    Institute of Scientific and Technical Information of China (English)

    田大强; 王宝安; 蒋平; 郑玉海

    2003-01-01

    中石油西气东输管道防腐公司利用中频感应加热炉对输气管道进行防腐处理,在中频炉投入运行时,却导致其他并联支路的无功补偿电容器组过流、损坏.通过对其中主要的中频炉设备的谐波状况所作测试的结果,分析了该谐波的特点及引起故障的原因,并介绍了相应的谐波治理方案.

  5. 尼泊金复合酯作为防腐剂在食品中的应用%The usage of complex paraben as antiseptic in the food field

    Institute of Scientific and Technical Information of China (English)

    张显久; 苏德俏; 沈健; 陈国安

    2007-01-01

    本文详细介绍了尼泊金酯作为食品防腐剂使用时适应pH值广、抑菌能力强等优点,但其在食品中使用时也存在着如分散性差、有舌麻感等缺点,本文通过合理的尼泊金酯复配,解决了尼泊金酯作为食品防腐剂的上述缺点,并取得了很好的效果.

  6. High Performance Liquid Chromatography in Determining Parabens Antiseptic in Foods%食品中4种对羟基苯甲酸酯类防腐剂的高效液相色谱测定法

    Institute of Scientific and Technical Information of China (English)

    仲岳桐; 柳其芳; 陈卫; 康莉

    2005-01-01

    目的建立样品前处理方法简单,灵敏度高,适于对食品中对羟基苯甲酸酯类进行测定的方法.方法使用带二极管阵列检测器的液相色谱仪进行分离检测.结果前处理方法简单易行,应用二极管阵列检测器能有效提高定性的可靠性和定量的准确性.该方法精密度、准确度与相关系数均符合的要求.结论方法测定重复性好,灵敏度高,定性定量准确,结果令人满意.

  7. 尼泊金复合酯作为防腐剂在食品中的应用%The usage of complex paraben as antiseptic in the food field

    Institute of Scientific and Technical Information of China (English)

    张显久; 苏德俏; 沈健; 陈国安

    2006-01-01

    本文详细介绍了尼泊金酯作为食品防腐剂使用时适应pH值广、抑菌能力强等优点,但其在食品中使用时也存在着如分散性差、有舌麻感等缺点,本文通过合理的尼泊金酯复配,解决了尼泊金酯作为食品防腐剂的上述缺点,并取得了很好的效果.

  8. 77 FR 11560 - Government-Owned Inventions; Availability for Licensing

    Science.gov (United States)

    2012-02-27

    ... antimicrobial activity against drug resistant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) and... populations. Antiseptics in hospital settings. Competitive Advantages Effective for commonly occurring...

  9. 29 CFR 1910.1030 - Bloodborne pathogens.

    Science.gov (United States)

    2010-07-01

    ... whose legally permitted scope of practice allows him or her to independently perform the activities... with 29 CFR 1910.20(e). (iv) The Exposure Control Plan shall be reviewed and updated at least annually... antiseptic hand cleanser in conjunction with clean cloth/paper towels or antiseptic towelettes....

  10. Drug: D07245 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D07245 Drug Nitroxoline (INN); Nitroxoline (TN) C9H6N2O3 190.0378 190.1555 D07245.gif Urinary tract antisept...ic ATC code: J01XX07 Anatomical Therapeutic Chemical (ATC) classification [BR:br083

  11. 重组人β防御素2在真皮多能干细胞中的表达及抗菌活性的测定%Expression of recombinant human β-defensin 2 in dermal multipotent stem cells and its antiseptic activity

    Institute of Scientific and Technical Information of China (English)

    李楠; 肖桃元; 粟永萍; 徐辉; 王军平; 宗兆文; 冉新泽; 董世武; 刘志君

    2006-01-01

    目的检测重组人β防御素2(human β-defensin 2, hBD2)腺病毒表达载体在大鼠真皮多能干细胞(dermal multipotent stem cells, dMSCs)中的表达,并观察重组hBD2的体外抗菌活性.方法将含有hBD2重组腺病毒转染dMSCs,RT-PCR、荧光免疫化学、Western blotting检测hBD2的表达情况,ELISA测定培养上清中hBD2的浓度,K-B纸片扩散法检测上清对大肠埃希菌、金黄色葡萄球菌和铜绿假单胞菌等标准菌株的杀灭效果.结果 RT-PCR、荧光免疫化学和Western blotting的结果显示转染后hBD2可在dMSCs中有效地表达,上清中hBD2的浓度为743.6 ng/ml,K-B纸片法显示HBD2对上述标准菌株有明显的杀灭效应.结论 hBD2重组腺病毒表达载体在dMSCs可高效表达,并对大肠埃希菌等标准菌株有杀灭效应.

  12. 活性银离子抗菌液预防胆道闭锁患儿发生鹅口疮的研究%Study on active silver ions of antiseptic solution for prevention of oral thrush occurred in children with biliary atresia

    Institute of Scientific and Technical Information of China (English)

    彭英

    2012-01-01

    [目的]探讨活性银离子抗菌液预防胆道闭锁患儿发生鹅口疮的效果.[方法]将80例胆道闭锁患儿随机分为对照组和实验组.对照组采用生理盐水清洁口腔,每日3次;实验组采用生理盐水清洁口腔后,喷活性银离子抗菌液,每日3次,从入院到出院.[结果]实验组口腔鹅口疮发生率低于对照组(P<0.05).[结论]预防性使用活性银离子抗菌液能降低胆道闭锁患儿口腔鹅口疮发生率.

  13. 乌蕨与黄连、黄芩、穿心莲抗菌活性的比较%The antiseptic activity of Stenoloma chusanum (L) Ching and its comparison to those of Coptis Chinenisis Franch, Scutellaria baicalensis Georgi,Andrographis paniculate (Bum.f) Nees

    Institute of Scientific and Technical Information of China (English)

    陈明; 段萍; 陈金秀

    2001-01-01

    为研究乌蕨的抗菌活性强度,采用二倍平皿稀释法将其与黄连、黄芩、穿心莲等抑菌结果进行比较.结果显示:乌蕨抗菌作用与黄连、黄芩相近,而强于穿心莲.认为乌蕨是一种具有较强抗菌活性的中草药.

  14. Security Evaluation for the Effect on Water Environment Quality Using Corrosion and Scale Inhibitor and Antiseptic Agent IMC-932-H.%IMC-932-H除氧缓蚀阻垢杀菌剂对水环境质量影响的安全性评估

    Institute of Scientific and Technical Information of China (English)

    苏娜; 王玉平

    2008-01-01

    依据美国环境保护局工业环境实验室提出的多介质环境目标值(MEG)及其综合排放指数对IMC-932-H除氧缓蚀阻垢杀菌剂在使用过程中一旦泄漏或排放,可能对水环境质量、人体健康和生态系统产生的影响进行了安全性评估.

  15. 德国赫尔曼O3水疗法配伍灭菌灵治疗外阴阴道假丝酵母菌病400例疗效观察%German Herrmann the O3 hydrotherapy blends the antiseptic spirit treatment vulva vagina rayon saccharomycetes to get sick 400 example curative effect observation

    Institute of Scientific and Technical Information of China (English)

    张海燕

    2010-01-01

    外阴阴道假丝酵母菌病是由假丝酵母菌引起的外阴阴道炎,我院自2007年开展应用德国赫尔曼O3水疗法配伍灭菌灵治疗外阴阴道假丝酵母菌病收到了满意的疗效,尤其能缓解急性、重症的外阴阴道假丝酵母菌病的瘙痒症状.

  16. Influence of low-intensity laser radiation upon the microflora of carious cavities and root canal

    Science.gov (United States)

    Shumilovitch, Bogdan R.; Nekrylov, Valery; Mazo, Leonid

    1995-04-01

    Laser stomatology- a relatively young branch of stomatology -has been developing actively lately. Bactericidal action of laser radiation enables to use it widely for processing carious cavities and root canals in the treatment of caries and its complications. 113 patients were studied by us. The 40 patients had antiseptic procedure of the caries cavity and then the procedure of laser therapy, so micro-organisms were found out in 26% cases. The 63 patients had antiseptic procedure only, so micro-organisms were found out in 70% cases. Control group were consisted of patients, where laser therapy was carried out without antiseptic remedies.

  17. Drug: D00863 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ANTISEPTICS AND DISINFECTANTS D08AG Iodine products D08AG02 Povidone-iodine D0086... antiinfectives D09AA09 Povidone-iodine D00863 Povidone-Iodine (JP16/USP) D11 OTHER DERMATOLOGICAL PREPARATI...ONS D11A OTHER DERMATOLOGICAL PREPARATIONS D11AC Medicated shampoos D11AC06 Povidone-iodine...X Other antiinfectives and antiseptics G01AX11 Povidone-iodine D00863 Povidone-Iodine (JP16/USP) R RESPIRATO...RY SYSTEM R02 THROAT PREPARATIONS R02A THROAT PREPARATIONS R02AA Antiseptics R02AA15 Povidone-iodine

  18. Eucalyptus

    Science.gov (United States)

    ... as an expectorant to loosen coughs, antiseptic, fever reducer, and in vaporizer fluids. Other uses include treatment ... leaf contains chemicals that might help control blood sugar. It also contains chemicals that might have activity ...

  19. 75 FR 36423 - Government-Owned Inventions; Availability for Licensing

    Science.gov (United States)

    2010-06-25

    ... tested for antimicrobial activity against drug resistant bacteria, methicillin- resistant Staphylococcus... bacterial infections in vivo, including for clinical and veterinary applications. Antiseptics in hospital... GTPase activity. Inhibit drug-susceptible and drug-resistant bacteria. Development Status: ]...

  20. Arthrography

    Science.gov (United States)

    ... the skin around the joint is cleansed with antiseptic and is often covered with a sterile surgical ... period is necessary. You may resume your usual activities and normal diet immediately after the exam. On ...

  1. 75 FR 81628 - Government-Owned Inventions; Availability for Licensing

    Science.gov (United States)

    2010-12-28

    ... tested for antimicrobial activity against drug resistant bacteria, methicillin- resistant Staphylococcus... bacterial infections in vivo, including for clinical and veterinary applications. Antiseptics in hospital... FtsZ GTPase activity. Inhibit drug-susceptible and drug-resistant bacteria. Development...

  2. 27 CFR 19.58 - Use of taxpaid distilled spirits to manufacture products unfit for beverage use.

    Science.gov (United States)

    2010-04-01

    ... PLANTS Administrative and Miscellaneous Provisions Activities Not Subject to This Part § 19.58 Use of...) Toilet, medicinal, and antiseptic preparations and solutions that are unfit for use for beverage...

  3. Drug: D07067 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ATIONS A01A STOMATOLOGICAL PREPARATIONS A01AB Antiinfectives and antiseptics for lo...D01AE05 Anatomical Therapeutic Chemical (ATC) classification [BR:br08303] A ALIMENTARY TRACT AND METABOLISM A01 STOMATOLOGICAL PREPAR

  4. Boils

    Science.gov (United States)

    ... the boil is very bad or comes back. Antibacterial soaps and creams cannot help much once a boil ... following may help prevent the spread of infection: Antibacterial soaps Antiseptic (germ-killing) washes Keeping clean (such as ...

  5. Lavender

    Science.gov (United States)

    ... was used as an antiseptic and for mental health purposes. Today, lavender is used as a folk or traditional remedy for anxiety, restlessness, insomnia, depression, headache, upset stomach, and hair loss. Lavender ...

  6. Topical wound-healing effects and phytochemical composition of heartwood essential oils of Juniperus virginiana L., Juniperus occidentalis Hook., and Juniperus ashei Juniperus Buchholz

    Science.gov (United States)

    Ethnobotanical surveys indicated that several Juniperus species are utilized as an antihelmintic, diuretic, stimulant, antiseptic, carminative, antirheumatic, antifungal and for wound healing. In the present study, essential oils obtained from heartwood samples of Juniperus virginiana L., J. occide...

  7. Blood culture

    Science.gov (United States)

    Culture - blood ... A blood sample is needed . The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  8. Testicular biopsy

    Science.gov (United States)

    Biopsy - testicle ... or at a hospital. The skin over the testicle is cleaned with a germ-killing (antiseptic) medicine. ... through the skin. A small piece of the testicle tissue is removed. The opening in the testicle ...

  9. Cancer treatment: preventing infection

    Science.gov (United States)

    ... cut, clean it right away with soap, warm water, and an antiseptic. Clean your cut this way every day until it forms a scab. Use gloves when gardening. There are often bacteria in soil. Stay away ...

  10. The Topical Evolution: Free Ions, Orthomolecular Agents, Phytochemicals, and Insect-Produced Substances

    OpenAIRE

    Conner-Kerr, Teresa

    2014-01-01

    Significance: A variety of topical antiseptic substances have been used historically to treat open wounds with suspected tissue infection or that are slow to heal. However, the effectiveness of these substances in treating infected or recalcitrant wounds remains controversial.

  11. Drug: D00882 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available BOLISM A01 STOMATOLOGICAL PREPARATIONS A01A STOMATOLOGICAL PREPARATIONS A01AB Antiinfectives and antisepti...cs for local oral treatment A01AB09 Miconazole D00882 Miconazole nitrate (JP16/USP)

  12. The Irrelevant Curriculum: A Review from Four Perspectives.

    Science.gov (United States)

    Ornstein, Allan C.

    1988-01-01

    Examines four varieties of curriculum irrelevance, including curricula that are fixed, antiseptic, trivial, and right answer-oriented. Suggests that educators need to stress curricular context and concern themselves more with process and less with products. Includes 10 references. (MLH)

  13. Aseptic tissue necrosis and chronic inflammation after irrigation of penetrating hand wounds using Octenisept®.

    Science.gov (United States)

    Franz, T; Vögelin, E

    2012-01-01

    Penetrating hand wounds are common and these are managed by thorough debridement. However, stab wounds without evidence of divided structures are often treated with irrigation using antiseptic substances, antibiotic therapy, and immobilization. Octenisept® (Schülke & Mayr Ltd) is a widely used antiseptic agent for disinfection of acute or chronic wounds. It has a broad spectrum of antiseptic efficacy and has become an antiseptic of first choice in many hospitals. Within a few months, four patients presented to us with chronic inflammation and severe tissue necrosis after irrigation of penetrating hand wounds with Octenisept®. Repeated surgery and debridement was required in all patients. Wound healing was prolonged and patients had persisting oedema. Penetrating hand wounds must not be irrigated with Octenisept®.

  14. Factors affecting bacterial counts during preparation of the hands for aseptic surgery.

    Science.gov (United States)

    Corder, K; Knowles, T G; Holt, P E

    2007-06-30

    This study was designed to compare the efficacy of four hand preparation techniques in removing bacteria from the hands preoperatively. The effect of bacteriological swabbing itself on bacterial counts was also investigated. The numbers of bacteria obtained from the dominant and non-dominant hands were also determined. The techniques all used 4 per cent chlorhexidine gluconate, and consisted of rubbing for five minutes with one application of antiseptic; rubbing for five minutes with five applications of antiseptic; rubbing for one minute with one application of antiseptic; and scrubbing with a brush for five minutes with one application of antiseptic. The results showed that the four techniques were equally effective at removing bacteria. There was no significant difference in the bacterial counts obtained from the dominant and non-dominant hands. The wearing of gloves for up to 30 minutes after scrubbing had no effect on the bacterial counts. Swabbing itself significantly reduced the number of bacteria cultured from the hands.

  15. Staph infections - self-care at home

    Science.gov (United States)

    ... include: Taking antibiotics Cleaning and draining the wound Surgery to remove an infected device ... DO NOT touch other people's bandages. Wash your hands well before and ... with antiseptic solution or wipes. Use clothing or a towel ...

  16. Drug: D07110 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ANTIINFECTIVES A07AX Other intestinal antiinfectives A07AX02 Acetarsol D07110 Acetarsol (INN) G GENITO URI...NARY SYSTEM AND SEX HORMONES G01 GYNECOLOGICAL ANTIINFECTIVES AND ANTISEPTICS G01A

  17. Drug: D07068 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available code: A01AB12 Anatomical Therapeutic Chemical (ATC) classification [BR:br08303] A ALIMENTARY TRACT AND METABOLISM A01 STOMA...TOLOGICAL PREPARATIONS A01A STOMATOLOGICAL PREPARATIONS A01AB Antiinfectives and antiseptics

  18. Drug: D06098 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available TC) classification [BR:br08303] A ALIMENTARY TRACT AND METABOLISM A01 STOMATOLOGICAL PREPARATIONS A01A STO...MATOLOGICAL PREPARATIONS A01AB Antiinfectives and antiseptics for local oral treatm

  19. PREPARATIVE SKIN PREPARATION AND SURGICAL WOUND INFECTION

    OpenAIRE

    Anjanappa; Arjun

    2015-01-01

    BACKGROUND AND OBJECTIVE: It is an established fact now that the normal skin of healthy human beings harbours a rich bacterial fl ora. Normally considered non - pathogenic , these organisms way be a potential source of infection of the surgical wound. Approximately 20% of the resident flora is beyond the reach of surgical scrubs and antiseptics. The goal of surgical preparation of the skin with antiseptics is to remove transient and pathogenic microorganism...

  20. Severe anaphylaxis: the secret ingredient.

    Science.gov (United States)

    Buergi, Andreas; Jung, Barbara; Padevit, Christian; John, Hubert; Ganter, Michael T

    2014-02-01

    In this case report, we describe a healthy urological patient who suffered severe intraoperative anaphylaxis to chlorhexidine, an ingredient contained in frequently used lubricants (Instillagel, Endosgel). Chlorhexidine is a well-known skin disinfectant and antiseptic component in mouthwash or other over the counter antiseptic pharmaceuticals. There is little awareness that commonly used lubricants may contain hidden chlorhexidine. After severe intraoperative anaphylaxis, it is important to investigate all potential (including hidden) agents that might have caused this life-threatening reaction. PMID:25611155

  1. ELIMINATION OF INTRACANAL INFECTION IN DOGS' TEETH WITH INDUCED PERIAPICAL LESIONS AFTER ROTARY INSTRUMENTATION: INFLUENCE OF DIFFERENT CALCIUM HYDROXIDE PASTES

    OpenAIRE

    Janir Alves Soares; Mário Roberto Leonardo; Léa Assed Bezerra da Silva; Mário Tanomaru Filho; Izabel Yoko Ito

    2006-01-01

    The aim of this study was to evaluate the antiseptic efficacy of rotary instrumentation associated with calcium hydroxide-based pastes prepared with different vehicles and antiseptics. Chronic periapical lesions were experimentally induced in 72 premolar root canals of four dogs. Under controlled asepsis, after initial microbiological sampling (A1), the root canals were instrumented using the ProFile system in conjunction with 5.25% sodium hypochlorite and the intracanal medication was placed...

  2. New strategies for preoperative skin antisepsis.

    Science.gov (United States)

    Ulmer, Miriam; Lademann, Juergen; Patzelt, Alexa; Knorr, Fanny; Kramer, Axel; Koburger, Torsten; Assadian, Ojan; Daeschlein, Georg; Lange-Asschenfeldt, Bernhard

    2014-01-01

    During the past decades, encouraging progress has been made in the prevention of surgical site infections (SSI). However, as SSI still occur today, strategic prevention measures such as standardized skin antisepsis must be implemented and rigorously promoted. Recent discoveries in skin physiology necessitate the development of novel antiseptic agents and procedures in order to ameliorate their efficacy. In particular, alternate target structures in the skin need to be taken into consideration for the development of the next generation of antiseptics. Recent investigations have shown that a high number of microorganisms are located within and in the close vicinity of the hair follicles. This suggests that these structures are an important reservoir of bacterial growth and activity in human skin. To date, it has not been fully elucidated to what extent conventional liquid antiseptics sufficiently target the hair follicle-related microbial population. Modern technologies such as tissue-tolerable plasma (TTP) have been tested for their potential antiseptic efficiency by reducing the bacterial load in the skin and in the hair follicles. First experiments using liposomes to deliver antiseptics into the hair follicles have been evaluated for their potential clinical application. The present review evaluates these two innovative methods for their efficacy and applicability in preoperative skin antiseptics.

  3. Polypragmasia in the therapy of infected wounds - conclusions drawn from the perspectives of low temperature plasma technology for plasma wound therapy.

    Science.gov (United States)

    Kramer, Axel; Hübner, Nils-Olaf; Weltmann, Klaus-Dieter; Lademann, Jürgen; Ekkernkamp, Axel; Hinz, Peter; Assadian, Ojan

    2008-11-03

    As long as a wound is infected, the healing process cannot begin. The indication for wound antiseptic is dependent on the interaction between the wound, the causative micro-organisms, and the host immune system. An uncritical colonisation is a condition whereby micro-organisms on a wound will proliferate, yet the immune system will not react excessively. Wound antiseptic is most often not necessary unless for epidemiologic reasons like colonisation with multi-resistant organisms. In most instances of a microbial contamination of the wound and colonisation, thorough cleaning will be sufficient.Bacterial counts above 10(5) to 10(6) cfu per gram tissue (critical colonisation) might decrease wound healing due to release of toxins, particularly in chronic wounds. Traumatic and heavily contaminated wounds therefore will require anti-infective measures, in particular wound antiseptic. In such situations, even a single application of an antiseptic compound will significantly reduce the number of pathogens, and hence, the risk of infection. If a wound infection is clinically manifest, local antiseptics and systemic antibiotics are therapeutically indicated.The prophylactic and therapeutic techniques for treatment of acute and chronic wounds (chemical antiseptics using xenobiotics or antibiotics, biological antiseptic applying maggots, medical honey or chitosan, physical antiseptic using water-filtered infrared A, UV, or electric current) mostly have been empirically developed without establishing a fundamental working hypothesis for their effectiveness.The most important aspect in controlling a wound infection and achieving healing of a wound is meticulous debridement of necrotic material. This is achieved by surgical, enzymatic or biological means e.g. using maggots. However, none of these methods (with some exception for maggots) is totally gentle to vital tissue and particularly chemical methods possess cytotoxicity effects.DERIVED FROM THE GENERAL PRINCIPLES OF

  4. Handwashing practices for the prevention of nosocomial infections.

    Science.gov (United States)

    Steere, A C; Mallison, G F

    1975-11-01

    Handwashing is generally considered the most important procedure in preventing nosocomial infections, because many types of these infections may be caused by organisms transmitted on the hands of personnel. Personnel should wash their hands before and after significant contact with any patient. The risk of personnel acquiring transient hand carriage of organisms is usually greatest after contact with excretions, secretions, or blood; patients at greatest risk are those undergoing surgery, those with catheters, and newborn infants. Although handwashing with an antiseptic agent between patient contacts is theoretically desirable, handwashing with soap, water, and mechanical friction are sufficient ro remove most transiently acquired organisms. Antiseptic agents may produce excessively dry skin if used frequently, and any regimen of handwashing that leads to dermatitis negates the purpose of handwashing. We favor antiseptics for handwashing before surgery and other high-risk invasive procedures and in the care of newborn infants but prefer soap and water for other handwashing.

  5. [Mouthwash solutions with microencapsuled natural extracts: Efficiency for dental plaque and gingivitis].

    Science.gov (United States)

    Vervelle, A; Mouhyi, J; Del Corso, M; Hippolyte, M-P; Sammartino, G; Dohan Ehrenfest, D M

    2010-06-01

    Mouthwash solutions are mainly used for their antiseptic properties. They currently include synthetic agents (chlorhexidine, triclosan, etc.) or essential oils (especially Listerine). Many natural extracts may also be used. These associate both antiseptic effects and direct action on host response, due to their antioxidant, immunoregulatory, analgesic, buffering, or healing properties. The best known are avocado oil, manuka oil, propolis oil, grapefruit seed extract, pycnogenol, aloe vera, Q10 coenzyme, green tea, and megamin. The development of new technologies, such as microencapsulation (GingiNat concept), may allow an in situ slow release of active ingredients during several hours, and open new perspectives for mouthwash solutions.

  6. Surgical Skills Beyond Scientific Management

    Science.gov (United States)

    Whitfield, Nicholas

    2015-01-01

    During the Great War, the French surgeon Alexis Carrel, in collaboration with the English chemist Henry Dakin, devised an antiseptic treatment for infected wounds. This paper focuses on Carrel’s attempt to standardise knowledge of infected wounds and their treatment, and looks closely at the vision of surgical skill he espoused and its difference from those associated with the doctrines of scientific management. Examining contemporary claims that the Carrel–Dakin method increased rather than diminished demands on surgical work, this paper further shows how debates about antiseptic wound treatment opened up a critical space for considering the nature of skill as a vital dynamic in surgical innovation and practice. PMID:26090737

  7. Design, synthesis, and antibacterial activities of neomycin-lipid conjugates: polycationic lipids with potent gram-positive activity.

    Science.gov (United States)

    Bera, Smritilekha; Zhanel, George G; Schweizer, Frank

    2008-10-01

    Aminoglycoside antibiotics and cationic detergents constitute two classes of clinically important drugs and antiseptics. Their bacteriological and clinical efficacy, however, has decreased recently due to antibiotic resistance. We have synthesized aminoglycoside-lipid conjugates in which the aminoglycoside neomycin forms the cationic headgroup of a polycationic detergent. Our results show that neomycin-C16 and neomycin-C20 conjugates exhibit strong Gram-positive activity but reduced Gram-negative activity. The MIC of neomycin-C16 (C20) conjugates against methicillin-resistant Staphylococcus aureus (MRSA) is comparable to clinically used antiseptics.

  8. Octenidine dihydrochloride: chemical characteristics and antimicrobial properties.

    Science.gov (United States)

    Assadian, Ojan

    2016-03-01

    The empiric use of antibiotics is being restricted due to the spread of antimicrobial resistance. However, topical antiseptics are less likely to induce resistance, owing to their unspecific mode of action and the high concentrations in which they can be used. One such antiseptic, octenidine dihydrochloride (OCT), can be used either prophylactically or therapeutically on the skin, mucosa and wounds. Evidence to support its use comes from in-vitro, animal and clinical studies on its safety, tolerability and efficacy. This article summarises the physical, chemical and antimicrobial properties of OCT in the context of wound care. PMID:26949863

  9. Rapid anti-pathogen response in ant societies relies on high genetic diversity

    DEFF Research Database (Denmark)

    Ugelvig, Line V; Kronauer, Daniel Jan Christoph; Schrempf, Alexandra;

    2010-01-01

    group-level resistance to pathogens relates directly to individual physiology, defence behaviour and social interactions. We investigated the effects of high versus low genetic diversity on both the individual and collective disease defences in the ant Cardiocondyla obscurior. We compared the antiseptic...... fungal spores from the nest, workers from homogeneous colonies only removed sick larvae late after infection. This difference was not caused by a reduced repertoire of antiseptic behaviours or a generally decreased brood care activity in ants from homogeneous colonies. Our data instead suggest...

  10. Alkylphenoxyalkylstannanes as biocidal additives to lubricants

    Energy Technology Data Exchange (ETDEWEB)

    Belov, P.S.; Gulo, R.A.; Komarova, N.N.; Korenev, K.D.; Poddubnyi, V.N.; Tsvetkov, O.N.

    1980-01-01

    The synthesis of akylphenoxyalkylstannanes with different numbers and lengths of akyl radicals on the tin atom and different structures and lengths of radicals on the aromatic ring is described. They were investigated as biocidal additives to lubricants. Alkylphenoxytriethylstannanes have the best protective properties. In 0.25% concentration (by wt) neither the structure nor the alkyl substituent (C/sub 1/-C/sub 16/) length has an effect on their antiseptic properties. From the results of the conducted studies, for antiseptization of lubricating compositions, the additive AFOTAS a reaction product of an industrial alkylphenol and bis(triethylol) oxide is recommended.

  11. 78 FR 34795 - Formaldehyde; Third-Party Certification Framework for the Formaldehyde Standards for Composite...

    Science.gov (United States)

    2013-06-10

    ... press fabrics, carpets, antiseptics, medicines, cosmetics, dishwashing liquids, fabric softeners, shoe...-laboratory testing comparisons for laboratories used by the TPC. 3. Recent activities related to this..., accredit, oversee, audit, and inspect both domestic and foreign TPCs--activities that would enable EPA...

  12. 78 FR 1574 - Regulatory Agenda

    Science.gov (United States)

    2013-01-08

    ... antiseptic products. Timetable: Action Date FR Cite NPRM (Healthcare) 06/17/94 59 FR 31402 Comment Period End... public more effectively participate in the Department's regulatory activity, and the Department welcomes... underlying medicine, public health, and social services. This agenda presents the rulemaking activities...

  13. FORMULATION AND EVALUATION OF HERBAL HAND WASH FROM MATRICARIA CHAMOMILLA FLOWERS EXTRACTS

    Directory of Open Access Journals (Sweden)

    Ali Heyam Saad

    2011-06-01

    Full Text Available Nasocomial infection has emerged as a critical issue in hospital care outcome, resulting in substantial morbidity and mortality. The hands of health care workers are the primary routes of transmission of infection to patients. Hence, it brings up the use of antiseptic for hand washing purposes. Many of the antiseptic available in market are alcohol based sanitizers which have some shortcomings or adverse effects. Their frequent use can lead to skin irritation. Chamomile is one of the most widely used and well-documented medicinal plants in the world. This study aimed to formulate effective herbal hand wash using Matricaria chamomilla (German chamomile flowers with emphasis on safety and efficacy and to avoid the risk posed by synthetic antimicrobials. Evaluation of the antimicrobial activity against skin pathogens of the prepared herbal hand wash was performed using disc diffusion method. Its efficacy was checked and compared with the commercial ones. Results revealed that chamomile soap formulation was more efficient in reducing the number of organisms from hands than the commercial antiseptic soaps thus it can be used as an antiseptic soap with less or no side effects.

  14. Drug: D04441 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available c ATC code: R02AA12 Anatomical Therapeutic Chemical (ATC) classification [BR:br08303] R RESPIRATORY SYSTEM R02 THROAT PREPARATION...S R02A THROAT PREPARATIONS R02AA Antiseptics R02AA12 Hexylresorcinol D04441 Hexylresorc

  15. A validated spectrofluorimetric method for the determination of nifuroxazide through coumarin formation using experimental design

    OpenAIRE

    El-Zaher, Asmaa Ahmed; Mahrouse, Marianne Alphonse

    2013-01-01

    Background Nifuroxazide (NF) is an oral nitrofuran antibiotic, having a wide range of bactericidal activity against gram positive and gram negative enteropathogenic organisms. It is formulated either in single form, as intestinal antiseptic or in combination with drotaverine (DV) for the treatment of gastroenteritis accompanied with gastrointestinal spasm. Spectrofluorimetry is a convenient and sensitive technique for pharmaceutical quality control. The new proposed spectrofluorimetric method...

  16. Healthcare-Wide Hazards: Surgical Suite

    Science.gov (United States)

    ... soon as feasible. If there has been no occupational exposure to blood or OPIM, use of an appropriate antiseptic hand cleanser is acceptable. Additional Information: FDA, NIOSH and OSHA Joint Safety Communication: Blunt-Tip Surgical Suture Needles Reduce Needlestick Injuries ...

  17. The Topical Evolution: Free Ions, Orthomolecular Agents, Phytochemicals, and Insect-Produced Substances.

    Science.gov (United States)

    Conner-Kerr, Teresa

    2014-08-01

    Significance: A variety of topical antiseptic substances have been used historically to treat open wounds with suspected tissue infection or that are slow to heal. However, the effectiveness of these substances in treating infected or recalcitrant wounds remains controversial. Recent Advances: Newly formulated topical antiseptics delivered through differing dressing technologies, such as ionic substances, hold the potential to limit the development of and treat antibiotic-resistant microbes in open wounds. Other topically delivered substances, such as insect-derived substances, orthomolecular agents, and phytochemicals, also present opportunities to optimize wound healing by decreasing tissue bioburden and facilitating the wound healing process. Critical Issues: Limited systemic perfusion of open wounds in individuals with certain diagnoses, such as peripheral arterial disease or necrotizing infection and the increasing number of antibiotic-resistant wound pathogens, suggests a continued role for topically applied antiseptic agents. Likewise, the failure of wounds to heal when treated with standard of care therapy opens the door to innovative treatment approaches that include the natural substances described in this article. Future Directions: Evidence for the use of select topical antiseptic agents from each of the aforementioned categories will be discussed in this article. Additional well-controlled clinical studies are needed to provide definitive recommendations for many of these topical agents.

  18. Involvement of Vacuolar Sequestration and Active Transport in Tolerance of Saccharomyces cerevisiae to Hop Iso-α-Acids

    NARCIS (Netherlands)

    Hazelwood, L.A.; Walsh, M.C.; Pronk, J.T.; Daran, J.M.

    2009-01-01

    The hop plant, Humulus lupulus L., has an exceptionally high content of secondary metabolites, the hop -acids, which possess a range of beneficial properties, including antiseptic action. Studies performed on the mode of action of hop iso--acids have hitherto been restricted to lactic acid bacteria.

  19. The danger of chlorhexidine in lignocaine gel: A case report of anaphylaxis during urinary catheterisation

    OpenAIRE

    Stewart, Michael; Lenaghan, Daniel

    2015-01-01

    This article describes a case of anaphylaxis secondary to chlorhexidine during urethral catheterisation. Despite little evidence for the use of antiseptic lubricants in preventing catheter-associated urinary tract infections, the distribution and use of such products continues to be widespread. Chlorhexidine-free lubricating gel is widely available and should be used for urological procedures wherever possible.

  20. Perioperative chlorhexidine allergy: Is it serious?

    OpenAIRE

    Abdallah, Claude

    2015-01-01

    Chlorhexidine is an antiseptic agent, commonly used, in many different preparations, and for multiple purposes. Despite its superior antimicrobial properties, chlorhexidine is a potentially allergenic substance. The following is a review of the current evidence-based knowledge of allergic reactions to chlorhexidine associated with surgical and interventional procedures.

  1. Wound infection caused by Lichtheimia ramosa due to a car accident

    NARCIS (Netherlands)

    Bibashi, Evangelia; de Hoog, G Sybren; Pavlidis, Theodoros E; Symeonidis, Nikolaos; Sakantamis, Athanasios; Walther, Grit

    2012-01-01

    A 32-year-old immunocompetent man sustained severe traumas contaminated with organic material due to a car accident. An infection caused by Lichtheimia ramosa at the site of contamination was early diagnosed and cured by multiple surgical debridement and daily cleansing with antiseptic solution only

  2. Wound infection caused by Lichtheimia ramosa due to a car accident

    NARCIS (Netherlands)

    E. Bibashi; G.S. de Hoog; T.E. Pavlidis; N. Symeonidis; A. Sakantamis; G. Walther

    2013-01-01

    A 32-year-old immunocompetent man sustained severe traumas contaminated with organic material due to a car accident. An infection caused by Lichtheimia ramosa at the site of contamination was early diagnosed and cured by multiple surgical debridement and daily cleansing with antiseptic solution only

  3. Bismuth toxicity in patients treated with bismuth iodoform paraffin packs.

    Science.gov (United States)

    Atwal, A; Cousin, G C S

    2016-01-01

    Bismuth is a heavy metal used in bismuth iodoform paraffin paste (BIPP) antiseptic dressings and in a number of other medical preparations. It can be absorbed systemically and cause toxicity. We report 2 cases of such neurotoxicity after it was used in operations on the jaws.

  4. Review on antibacterial characteristics of bridge engineering biomaterials.

    Science.gov (United States)

    Zhao, Qing-Qing; Chen, Meng-Yao; He, Rui-Lin; Zhang, Zhong-Feng; Ashraf, Muhammad Aqeel

    2016-01-01

    This review summarizes the research on timber construction materials used in bridge construction. It focuses on the application of antiseptic treatments and the use of timber engineering materials in decks and bridges. This review also provides an overview on the future research and prospects of engineered timber materials. PMID:26858558

  5. The Biochemical Impact of Surgery and Anesthesia

    NARCIS (Netherlands)

    J.W. Hol (Jaap Willem)

    2014-01-01

    markdownabstract__Abstract__ General anesthesia has been considered by some medical historians as one of the most important contributions to modern medicine second to perhaps the concept of antiseptic medicine and hygiene. The first historical mention of a deep unnatural sleep so that surgery can t

  6. The Topical Evolution: Free Ions, Orthomolecular Agents, Phytochemicals, and Insect-Produced Substances.

    Science.gov (United States)

    Conner-Kerr, Teresa

    2014-08-01

    Significance: A variety of topical antiseptic substances have been used historically to treat open wounds with suspected tissue infection or that are slow to heal. However, the effectiveness of these substances in treating infected or recalcitrant wounds remains controversial. Recent Advances: Newly formulated topical antiseptics delivered through differing dressing technologies, such as ionic substances, hold the potential to limit the development of and treat antibiotic-resistant microbes in open wounds. Other topically delivered substances, such as insect-derived substances, orthomolecular agents, and phytochemicals, also present opportunities to optimize wound healing by decreasing tissue bioburden and facilitating the wound healing process. Critical Issues: Limited systemic perfusion of open wounds in individuals with certain diagnoses, such as peripheral arterial disease or necrotizing infection and the increasing number of antibiotic-resistant wound pathogens, suggests a continued role for topically applied antiseptic agents. Likewise, the failure of wounds to heal when treated with standard of care therapy opens the door to innovative treatment approaches that include the natural substances described in this article. Future Directions: Evidence for the use of select topical antiseptic agents from each of the aforementioned categories will be discussed in this article. Additional well-controlled clinical studies are needed to provide definitive recommendations for many of these topical agents. PMID:25126473

  7. Advances on the Technologies of Keeping Pickled Vegetables Fresh%酱腌菜防腐保鲜技术研究进展

    Institute of Scientific and Technical Information of China (English)

    张爽

    2011-01-01

    The technologies of keeping pickled vegetables fresh were summarized in this paper. The usual ways to keep pickled vegetgbles fresh included salting, killing bacteria with a high temperature, using antiseptic and vacuum packing, as well as some specific methods such as microwave sterilizing, ultrasonic cleansing, adding biologic antiseptic and compound antiseptic and the application of railing technology. By expanding the physical fresh-keeping technologies and applying compound antiseptic and railing technologies scientifically, low-salt pickled vegetables producers could prolong their products' quality guarantee period and improve quality safety.%对酱腌莱防腐保鲜技术进行了综述.酱腌菜防腐保鲜的传统方法包括盐腌法、高温灭菌法、化学防腐剂和真空包装法,特殊方法包括微波灭菌技术、超声波清洗技术、生物防腐剂和复合防腐剂的运用以及栅栏技术的运用.酱腌菜生产企业可以通过拓展酱腌莱的物理保鲜技术,结合工艺控制科学复合使用防腐剂,利用栅栏防腐技术综合提高低盐酱菜保质期和食用安全性.

  8. THE STUDY OF RESISTENCE OF STAPHYLOCOCCUS AUREUS STRAINS TO ANTIMICROBIALS

    Directory of Open Access Journals (Sweden)

    Nazarchuk GG

    2012-12-01

    Full Text Available In the research work the results of the study of resistance forming to antibiotics, antiseptics and decametoxine composition with modified polysaccharides in S.aureus strains are presented. The development of resistance to penicillins, cephalosporins, glycopeptides, macrolides is shown. Slow forming of resistance to decasan and decametoxine composition with carboxymethylamylum, oxyethylcellulose was determined.

  9. Post-operative effects on silver coated tumor endoprosthesis and biofilm prophylaxis systems

    Science.gov (United States)

    Heinrich, L.; Ahrens, H.; Wahrenburg, M.; Pajaziti, B.; Kurzina, I. A.

    2015-11-01

    Despite the high state of the art in limp replacement and the reconstructive tumor surgery, failures cannot be excluded. Investigations on post-operative effects on explanted silver coated modules of MUTARS® prostheses have disclosed changes in the silver surface. Both, the silver coatings and the perioperative lavage with antiseptics reduce efficiently the risks of infections.

  10. SPRINGBOARDS, TEXTS THE STUDENTS STEAL.

    Science.gov (United States)

    HARRIS, CHARLES

    ON THE PREMISE THAT GHETTO YOUTH ARE NONREADERS MAINLY BECAUSE MOST READING MATERIALS ARE UNINSPIRED, MIDDLE-CLASS, AND ANTISEPTIC, THE SPRINGBOARDS READING PROGRAM WAS DEVELOPED. PRESENTLY IMPLEMENTED IN HARLEM (NEW YORK CITY) "STREET ACADEMIES," THE PROGRAM UTILIZES A SERIES OF FOUR-PAGE BOOKLETS DESIGNED TO INTEREST DISADVANTAGED MALE DROPOUTS…

  11. Antifungal compounds from Piper species

    Science.gov (United States)

    Piper is a big genus of the plant family Piperaceae, with more than 700 species widely distributed in the tropical and subtropical regions of the world. Some species are used in folk medicine as analgesics, antiseptics, insecticides, and antimicrobials or for the treatment of toothache, haemorrhoid...

  12. Staphylococcal scalded skin syndrome hos voksne

    DEFF Research Database (Denmark)

    Mikkelsen, Carsten Sauer; Mikkelsen, Dorthe Bisgaard; Jensen, Thøger Gorm;

    2010-01-01

    parts of her body. The bullae ruptured easily and left a erythematous base. The histopathological changes were characteristic for adult SSSS. The patient was well-treated with intravenous dicloxacillin, topical antibiotic and antiseptic treatment. The patient had marked thrombocytosis, but no interest...

  13. Teaching Undergraduates Psychoeducational Management Skills: From Theory to Practice with Preschool Children.

    Science.gov (United States)

    Molloy, Christine

    1989-01-01

    Described are positive classroom practices used for behavior management at a child development center, including proximity control, signal interference, touch control, decoding skills, antiseptic bouncing, planned ignoring with positive reinforcement, and supporting cooperative play. Also described is a situational crisis controlled through use of…

  14. Toxicological Problems with the Redy System

    DEFF Research Database (Denmark)

    Broch Møller, B.; Bahnsen, M.; Solgaard, Per Bent;

    1976-01-01

    be substituted by another antiseptic. Of special interest was the finding of a very high concentration of boron in the dialysate. It is suggested that this metal, which is a potentially toxic substance, may emanate from the patient. It is concluded that use of the REDY system carries no obvious toxicological...

  15. Drug: D03347 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available fungal ATC code: G01AA04 polyene macrolide antibiotic Anatomical Therapeutic Chemical (ATC) classification [BR:br08303] G GENITO URIN...ARY SYSTEM AND SEX HORMONES G01 GYNECOLOGICAL ANTIINFECTIVES AND ANTISEPTICS G01A A

  16. Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury

    OpenAIRE

    Renata Grespan; Rafael Pazinatto Aguiar; Frederico Nunes Giubilei; Rafael Rocco Fuso; Marcio José Damião; Expedito Leite Silva; Jane Graton Mikcha; Luzmarina Hernandes; Ciomar Bersani Amado; Roberto Kenji Nakamura Cuman

    2014-01-01

    Acute liver damage caused by acetaminophen overdose is a significant clinical problem and could benefit from new therapeutic strategies. Objective. This study investigated the hepatoprotective effect of Thymus vulgaris essential oil (TEO), which is used popularly for various beneficial effects, such as its antiseptic, carminative, and antimicrobial effects. The hepatoprotective activity of TEO was determined by assessing serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), ...

  17. PREPARATIVE SKIN PREPARATION AND SURGICAL WOUND INFECTION

    Directory of Open Access Journals (Sweden)

    Anjanappa

    2015-01-01

    Full Text Available BACKGROUND AND OBJECTIVE: It is an established fact now that the normal skin of healthy human beings harbours a rich bacterial fl ora. Normally considered non - pathogenic , these organisms way be a potential source of infection of the surgical wound. Approximately 20% of the resident flora is beyond the reach of surgical scrubs and antiseptics. The goal of surgical preparation of the skin with antiseptics is to remove transient and pathogenic microorganisms on the skin surface and to reduce the resident flora to a low level. Povidone iodine (I odophors and chlorhexidine are most often used antiseptics for pre - operative skin preparation. OBJECTIVES : To evaluate the efficacy of povidone iodine alone and in combination with antiseptic agent containing alcoholic chlorhexidine in preoperative skin p reparation by taking swab culture. (2 To compare the rate of postoperative wound infection in both the groups. METHODS: One hundred patients (fifty in each group undergoing clean elective surgery with no focus of infection on the body were included in th e study. The pre - operative skin preparation in each group is done with the respective antiseptic regimen. In both the groups after application of antiseptics , sterile saline swab culture was taken immediately from site of incision. In cases which showed gr owth of organisms , the bacteria isolated were identified by their morphological and cultural characteristics. Grams staining , coagulase test and antibiotic sensitivity test were done wherever necessary and difference in colonization rates was determined as a measure of efficacy of antiseptic regimen. RESULTS: The results of the study showed that when compared to povidone iodine alone , using a combination of povidone iodine and alcoholic solution of chlorhexidine , the colonization rates of the site of incisi on were reduced significantly. As for the rate of post - operative wound infection , it is also proven that wound infections are also

  18. Pitfalls in efficacy testing – how important is the validation of neutralization of chlorhexidine digluconate?

    Directory of Open Access Journals (Sweden)

    Heisig Peter

    2008-12-01

    Full Text Available Abstract Background Effective neutralization of active agents is essential to obtain valid efficacy results, especially when non-volatile active agents like chlorhexidine digluconate (CHG are tested. The aim of this study was to determine an effective and non-toxic neutralizing mixture for a propan-1-ol solution containing 2% CHG. Methods Experiments were carried out according to ASTM E 1054-02. The neutralization capacity was tested separately with five challenge microorganisms in suspension, and with a rayon swab carrier. Either 0.5 mL of the antiseptic solution (suspension test or a saturated swab with the antiseptic solution (carrier test was added to tryptic soy broth containing neutralizing agents. After the samples were mixed, aliquots were spread immediately and after 3 h of storage at 2 – 8°C onto tryptic soy agar containing a neutralizing mixture. Results The neutralizer was, however, not consistently effective in the suspension test. Immediate spread yielded a valid neutralization with Staphylococcus aureus, Staphylococcus epidermidis and Corynebacterium jeikeium but not with Micrococcus luteus (p Candida albicans (p Staphylococcus epidermidis (p Corynebacterium jeikeium (p = 0.044. In the carrier test, the neutralizing mixture was found to be effective and non toxic to all challenge microorganisms when spread immediately. However, after 3 h storage of the neutralized active agents significant carry-over activity of CHG against Micrococcus luteus (p = 0.004; Tukey HSD was observed. Conclusion Without effective neutralization in the sampling fluid, non-volatile active ingredients will continue to reduce the number of surviving microorganisms after antiseptic treatment even if the sampling fluid is kept cold straight after testing. This can result in false-positive antiseptic efficacy data. Attention should be paid during the neutralization validation process to the amount of antiseptic solution, the storage time and to the choice of

  19. Perbandingan Antiseptik Chlorhexidine Alkohol dengan Povidone Iodine terhadap Penurunan Pertumbuhan Koloni Bakteri pada Kateter Epidural yang Dipasang di Kamar Operasi Rumah Sakit Dr. Hasan Sadikin Bandung

    Directory of Open Access Journals (Sweden)

    Andie Muhari Barzah

    2016-04-01

    Full Text Available Chlorhexidine-alcohol and povidone iodine are commonly used as antiseptic solutions in epidural anesthesia. Aseptic and antiseptic procedures must be performed before any epidural procedure to lower infection complications after the procedure. The objective of this study was to investigate the lowest bacterial growth on epidural catheter after chlorhexidine-alcohol or povidone iodine application as the antiseptic solution in Dr. Hasan Sadikin General Hospital Bandung. This was a single blind randomized controlled trial on 78 patients underwent epidural anesthesia in the period of November 2014–February 2015. The subjects were divided in to 2 groups, each consisted of 38 patients. Aseptic and antiseptic procedures were performed using chlorhexidine-alcohol or povidone iodine with skin swab culture collected before and after the procedure. Epidural catheter culture was performed in the 3rd days after the installment. Data were analyzed using t-test, chi-square test, and Mann Whitney test. The result of this study showed positive bacterial cultures prior to aseptic and antiseptics, 33 from 38 in chlorhexidine-alcohol and 35 from 38 in povidone iodine. Positive bacterial cultures became 4 from 38, both in chlorhexidine-alcohol and povidone iodine. Positive bacterial culture on the 3rd days after epidural catheter placement was 7 from 38 after chlorhexidine-alcohol and 5 from 38 after povidone iodine (p< 0.05. It is concluded that the ability of chlorhexidine-alcohol to reduce bacterial growth on epidural catheter is lower than povidone-iodine with regards to reducing infection after catheter epidural installment.

  20. Daily Bathing with Chlorhexidine and Its Effects on Nosocomial Infection Rates in Pediatric Oncology Patients.

    Science.gov (United States)

    Raulji, Chittalsinh M; Clay, Kristin; Velasco, Cruz; Yu, Lolie C

    2015-01-01

    Infections remain a serious complication in pediatric oncology patients. To determine if daily bathing with Chlorhexidine gluconate can decrease the rate of nosocomial infection in pediatric oncology patients, we reviewed rates of infections in pediatric oncology patients over a 14-month span. Intervention group received daily bath with Chlorhexidine, while the control group did not receive daily bath. The results showed that daily bath with antiseptic chlorhexidine as daily prophylactic antiseptic topical wash leads to decreased infection density amongst the pediatric oncology patients, especially in patients older than 12 years of age. Furthermore, daily chlorhexidine bathing significantly reduced the rate of hospital acquired infection in patients older than 12 years of age. The findings of this study suggest that daily bathing with chlorhexidine may be an effective measure of reducing nosocomial infection in pediatric oncology patients.

  1. Application of Microbiological Test in Cream Cosmetics by Membrane Filter Method%薄膜过滤法在膏霜类化妆品微生物检验中的应用研究

    Institute of Scientific and Technical Information of China (English)

    庞燕军; 赵晓冬; 郭春梅; 矫筱蔓

    2011-01-01

    采用薄膜过滤法去除化妆品中防腐剂的抗菌活性,完善膏霜类化妆品微生物检验方法.该方法可以有效地去除化妆品中防腐剂的抗菌作用,使加菌回收率达到满意的效果,方法准确、可靠.%The filtration method was used to inhibit the residual antimicrobial activity of antiseptic in cream cosmetics to improve the microbial test method for cream cosmetics. The antimicrobial activity of antiseptic in cream cosmetics could be eliminated effectively to improve the recovery of bacterium by this method, and it is an accurate and reliable method.

  2. Application of neutralization method in detergent microbiology test%中和法在洗洁精微生物检测中的应用

    Institute of Scientific and Technical Information of China (English)

    江丹; 钟关; 刘翅; 张利萍

    2012-01-01

    The neutralization method was used to inhibit the residual antimaicrobial activity of antiseptic in detergent to improve the microbial test method for detergent.The antimicrobial activity of antiseptic in detergent was eliminated effectively and the recovery of bacterium improved in this method.It is accurate and reliable.%采用中和法中和洗洁精中的防腐剂,完善洗洁精的微生物检验方法。该方法可以有效去除洗洁精中防腐剂的抗菌作用,使加菌回收率达到满意的效果,方法准确、可靠。

  3. Composite Scaffolds Based on Silver Nanoparticles for Biomedical Applications

    Directory of Open Access Journals (Sweden)

    Jenel Marian Patrascu

    2015-01-01

    Full Text Available This paper presents the synthesis, characterisation, and in vitro testing of homogenous and heterogeneous materials containing silver nanoparticles (nanoAg. Three types of antiseptic materials based on collagen (COLL, hydroxyapatite (HA, and collagen/hydroxyapatite (COLL/HA composite materials were obtained. The synthesis of silver nanoparticles was realized by chemical reaction as well as plasma sputtering deposition. The use of chemical reduction allows the synthesis of homogenous materials while the plasma sputtering deposition can be easily used for the synthesis of homogeneous and heterogeneous support. Based on the in vitro assays clear antiseptic activity against Escherichia coli was relieved even at low content of nanoAg (10 ppm.

  4. Current Approaches for the Prevention of Surgical Site Infections

    Directory of Open Access Journals (Sweden)

    Sander Florman

    2007-01-01

    Full Text Available Surgical site infections (SSIs are the most common type of nosocomial infection among surgical patients and are commonly caused by the patients’ own microbial flora. The prevalence of SSI is a major concern because of the associated increase in the incidence of morbidity and mortality, length of hospitalization and cost of care for postoperative patients. Key factors that determine whether patients are at risk for developing SSI include the inherent potential contamination of the surgical site, the duration of the operation and the individual patient susceptibility. Preventive preoperative measures that can reduce the risk of SSIs include administration of antimicrobial prophylaxis, proper utilization of skin antiseptic agents for both the patient and the surgical team, proper patient preoperative hair removal and the policy of canceling elective procedures when remote skin, urinary or pulmonary infections occur. This paper will review the efficacy and safety of available antiseptic agents, as well as discuss patient-specific prevention strategies.

  5. No need to change the skin knife in modern arthroplasty surgery

    DEFF Research Database (Denmark)

    Ottesen, C; Skovby, A; Troelsen, A;

    2014-01-01

    to conflicting conclusions regarding discarding the skin knife or not. This study evaluates the prevalence of contamination of a separate skin knife using modern antiseptic technique in primary THA and TKA without laminar airflow. Three knives from each primary THA and TKA surgery in non-laminar airflow...... operating rooms were collected: one used for the skin, one used for deeper tissues and one control knife. A total of 831 knife blades from 277 patients were cultured 12 days. Contamination of the skin knife was found in eight patients (2.8 %), contamination of the "deep" knife in five patients (1.......8 %) and contamination of the control knife in five patients (1.8 %). No patient developed an infection with 1-year follow-up. Our findings suggest a very low rate of contamination of the skin knife using modern antiseptic technique without laminar airflow and/or plastic adhesive draping and do not support the use...

  6. [Outbreak due to Serratia marcescens associated with intrinsic contamination of aqueous chlorhexidine].

    Science.gov (United States)

    Hervé, Beatrice; Chomali, May; Gutiérrez, Cecilia; Luna, Mariana; Rivas, Jeannette; Blamey, Rodrigo; Espinoza, Ricardo; Izquierdo, Giannina; Cabezas, Catalina; Alvarez, Claudia; de la Fuente, Sebastián

    2015-10-01

    Serratia marcescens is a widely distributed gram-negative rod, often associated to nosocomial infections. Some outbreaks linked to contaminated antiseptic solutions have been reported. In this study we report a nosocomial outbreak of surgical site infection and catheter insertion site infection due to S. marcescens. 33 patients with positive cultures were studied after an index case was identified. Epidemiological, microbiological and molecular analysis demostrated an intrinsic contamination of alcohol free chlorhexidine solution as causal factor. Positive cultures were associated with 13 clinical infections, 9 colonized patients, 6 pseudobacteremia episodes and 5 patients without documented exposure. Hospital and national recall of contaminated chlorhexidine solution was performed after this study. Intrinsic contamination of antiseptic solutions is an infrequent cause of nosocomial infections with major epidemiological relevance.

  7. Drug utilization pattern in OPD of government dental college and hospital, Aurangabad

    Directory of Open Access Journals (Sweden)

    M. D. Kulkarni

    2013-02-01

    Full Text Available Background: To study drug prescription pattern in dental OPD of Government Dental College and Hospital. Methods: 100 prescriptions were screened & analyzed as per the study parameters from OPD of Government Dental College & Hospital, Aurangabad. Groups of drugs commonly prescribed, like antibiotic, analgesics & others prescription were recorded. Results: Most common groups of drugs prescribed by dental surgeons were NSAIDs, antimicrobials, antiseptics and multivitamins. NSAIDs- 86%; Antimicrobials- 85%; Antiseptics- 8.6%; Multivitamins- 12.3%. The average number of drugs prescribed per patient was 3 and the average number of antimicrobial prescribed per patient was 1. Conclusions: For rational prescribing of drugs there is a need of mass-awareness amongst dental surgeons about the good prescribing by following 6 steps of WHO program on rational use of drugs. [Int J Basic Clin Pharmacol 2013; 2(1.000: 69-70

  8. Combined Treatment Of Purulent Wounds With CO2 Laser

    Science.gov (United States)

    Tolstych, P. I.; Skobelkin, O. K.; Derbenev, V. A.

    1988-06-01

    The authors have worked out a programme for combined treatment of purulent wounds with CO2 laser. It consists of following components: a) laserinsision of suppurative wounds,necrectomy,or irradiation of the incised wound with the defocused laser beam; b )diplication of proteinerses and antiseptics immobilized on the textile dressing and dranaige materials; c) applications of biocompatible materials with the immobilized antiseptics and enzyme agents; d) application of low energy laser irradiation in the postoperative period; e) lavage of the sutured wound if indicated. The points of the above-mentioned programme can be used separately as independed methods in several thousands of patients. Their combined applications has been studied on 400 patients. Such treatment allowed to refuse the antibioticotherapy in 98,3% of cases,to reduce the terms of trestment in I,5-2 % times as compared with traditional methods, and to low significantely costs.

  9. Chlorhexidine--pharmacology and clinical applications.

    Science.gov (United States)

    Lim, K-S; Kam, P C A

    2008-07-01

    Chlorhexidine is a widely used skin antisepsis preparation and is an ingredient in toothpaste and mouthwash. It is an especially effective antiseptic when combined with alcohol. Its antimicrobial effects persist because it is binds strongly to proteins in the skin and mucosa, making it an effective antiseptic ingredient for handwashing, skin preparation for surgery and the placement of intravascular access. Catheters impregnated with chlorhexidine and antimicrobial agents can reduce the incidence of catheter-related bloodstream infections. Contact dermatitis related to chlorhexidine is not common in health care workers. The incidence of contact dermatitis to chlorhexidine in atopic patients is approximately 2.5 to 5.4%. Acute hypersensitivity reactions to chlorhexidine are often not recognised and therefore may be underreported. This review discusses the pharmacology, microbiology, clinical applications and adverse effects of chlorhexidine.

  10. BACTERIAL MICROORGANISMS ASSOCIATED WITH THE PLANT TISSUE CULTURE: IDENTIFICATION AND POSSIBLE ROLE (review)

    OpenAIRE

    S.E. DUNAEVA; Yu, S.

    2015-01-01

    Effective sterilization of plant explants and antiseptics rules compliance do not exclude the presence of so-called covert (endophytic) bacteria in in vitro cultures. But the role of these bacteria in tissues cultures has been not enough studied whereas it was related to the explants regeneration capacity and the possibility of animal and human cells transformation under in vitro cultivation. Bacterial strains pathogenic to humans can be stably maintained in cultivated tissues and ex vitro pl...

  11. [Contact allergy to essential oils].

    Science.gov (United States)

    Selvåg, E; Holm, J O; Thune, P

    1995-11-10

    Ethereal oils are widely used, in food, toothpaste, as flavouring agents in perfumes and cosmetics, and in dermatological treatment, as antiseptics, adstringenta, antipruritic tinctures, lotions and pomades. The use of ethereal oils seems to have increased in recent years, probably due to a growing interest in alternative medicine, and especially aromatherapy. By describing the cases of three patients with allergic contact dermatitis, we wish to point out possible unwanted side effects of using ethereal oils. PMID:7491578

  12. Evaluation of Anti-Inflammatory Activity of Citrus latifolia Tanaka Essential Oil and Limonene in Experimental Mouse Models

    OpenAIRE

    Raquel Kummer; Fernanda Carolina Fachini-Queiroz; Camila Fernanda Estevão-Silva; Renata Grespan; Expedito Leite Silva; Ciomar Aparecida Bersani-Amado; Roberto Kenji Nakamura Cuman

    2013-01-01

    The genus Citrus (Rutaceae) includes several species of plants that produce some of the most cultivated fruits in the world, providing an appreciable content of essential oil. In folk medicine, they are used as a cholagogue, antipyretic, anti-inflammatory, sedative, and antitoxic effects. Lemon essential oil has been used since ancient times for its antiseptic, carminative, diuretic, and eupeptic effects. In this study, we investigated the anti-inflammatory activity of Citrus latifolia Tanaka...

  13. The efficacy of mupirocin ointment and chlorhexidine body scrubs in the eradication of nasal carriage of Staphylococcus aureus among patients undergoing long-term hemodialysis

    OpenAIRE

    Watanakunakorn, C; Brandt, J.; Durkin, P; Santore, S.; Bota, B.; Stahl, C.

    1992-01-01

    textabstractPatients undergoing long-term hemodialysis have a high prevalence of Staphylococcus aureus nasal carriage, which may lead to serious infections. Mupirocin ointment has been used intranasally to eradicate S. aureus carriage in health human volunteers and health care workers. Chlorhexidine, an antiseptic with excellent antistaphylococcal activity, is widely used for handwashing and skin cleansing. METHODS: Anterior nares cultures were obtained from patients older than 18 years who w...

  14. Bioactivity and chemical characterization of phenolic and anthocyanin extracts of Arbutus unedo L. wild fruits

    OpenAIRE

    Guimarães, Rafaela; Barros, Lillian; Ricardo C. Calhelha; Dueñas, Montserrat; Carvalho, Ana Maria; Queiroz, Maria João R. P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.

    2013-01-01

    Phenolic metabolites are common constituents of fruits and vegetables, and the interest of plant phenolic extracts derives from the evidence of their potent antioxidant activity and their wide range of pharmacologic properties including anticancer, antimicrobial and platelet aggregation inhibition activities. The fruits of Arbutus unedo L. are eaten raw or made in liqueurs (1 ]. In traditional folk medicine, they are reported as having antiseptic, diuretic and laxative effects, be...

  15. Efficacy of diode laser for treating acne keloidalis nuchae

    OpenAIRE

    Shah Girish

    2005-01-01

    Acne keloidalis nuchae is usually treated with oral antibiotics, local antiseptics or intralesional steroids but with limited success. I assessed the efficacy of diode laser for treating the inflammatory and keloidal papules of acne keloidalis nuchae in two cases. The lesions in both the cases showed about 90 to 95% clearance after 4 treatment sessions at one to one and half month intervals. No new lesions were observed during the follow up period of six months after the last laser tre...

  16. Efficacy of octenidine dihydrochloride and 2-phenoxyethanol in the topical treatment of inflammatory acne:

    OpenAIRE

    Aberer, W.; Kränke, Birger; Mayr-Kanhäuser, Sigrid

    2008-01-01

    Background: With the increase in antibiotic-resistant strains of microorganisms in acne lesions, the search for alternative treatment methods has become important. We studied the efficacy of a combination of the antiseptic substances octenidine dihydrochloride and 2-phenoxyethanol (O/P) in mild to moderate inflammatory acne vulgaris. Methods: Thirty patients were instructed to apply O/P once or twice daily for a 6-week treatment period. Determination of efficacy included the numerical documen...

  17. Preparation and Antimicrobial Activity of s-Triazine Hydrazones of 7-Hydroxy Coumarin and Their Metal Complexes

    Directory of Open Access Journals (Sweden)

    G. R. Jani

    2009-01-01

    Full Text Available Metal complexes of 7-hydroxy coumarin hydrazone of s-triazine derivatives derived from 7-hydroxy-8-aceto-N-(4',6’-dichloro-1',3',5'-s-triazine coumarin hydrazone and transition metals have been synthesized and screened for their antibacterial, antifungal and antiseptic activity. The geometry of the complexes has been proposed. The ligand system co-ordinates with the metal ion in a bidentate manner through the nitrogen atom of hydrazone group.

  18. Tablets with thyme (Thymus Vulgaris L extracts

    Directory of Open Access Journals (Sweden)

    Zeković Zoran P.

    2002-01-01

    Full Text Available The strongly antiseptic and antifungal activities of thyme is mainly due to the presence of phenolic compounds, thymol and carvacrol. Thyme extracts essential oil, extract obtained using 70% ethanol and extract obtained by supercritical fluid extraction (SFE using carbon dioxide, were incorporated in the most useful form of pharmaceutical products - tablets. The work is concerned with the characterisation of the obtained tablets containing thyme extracts.

  19. Preparation of hydrogel by radiation for the healing of diabetic ulcer

    Science.gov (United States)

    Nho, Young-Chang; Park, Jong-Seok; Lim, Youn-Mook

    2014-01-01

    Honey has been used in wound care for thousands of years. The major advantage of honey in wound care is the high osmotic activity, which accelerates the debridement of necrotic tissue and procures an antibacterial effect. It has been reported that the ancient Greeks and Romans used honey as a topical antiseptic for sores and skin ulcers. The aims of this study were to evaluate the antibacterial activities and the healing effect for diabetic ulcers from carboxymethyl cellulose (CMC) hydrogel involving honey.

  20. Hepatitis C Virus Maintains Infectivity for Weeks After Drying on Inanimate Surfaces at Room Temperature: Implications for Risks of Transmission

    OpenAIRE

    Paintsil, Elijah; Binka, Mawuena; Patel, Amisha; Lindenbach, Brett D.; Heimer, Robert

    2013-01-01

    Background. Healthcare workers may come into contact with fomites that contain infectious hepatitis C virus (HCV) during preparation of plasma or following placement or removal of venous lines. Similarly, injection drugs users may come into contact with fomites. Hypothesizing that prolonged viability of HCV in fomites may contribute significantly to incidence, we determined the longevity of virus infectivity and the effectiveness of antiseptics.

  1. COMPARATIVE, QUALITATIVE AND QUANTITATIVE CHEMOTYPIC CHARACTERIZATION AMONG NORTH INDIAN TRIBULUS TERRESTRIS

    OpenAIRE

    Kumar Ashwani; Bhardwaj Ashish

    2012-01-01

    In India, Several medicinal plant species are used in herbal drug industries, whereas Tribulus terrestris extract has an ancient tradition in folk medicine and in ayurveda as a diuretic, mood enhancer, antiseptic and anti-inflammatory agent. Due to few phytochemical and pharmacological studies, there are no quality criteria for this Tribulus terrestris species as raw material. In this work, we present unique fingerprints of six samples of Tribulus terrestris population relating to the presenc...

  2. Chlorine dioxide is a size-selective antimicrobial agent.

    Directory of Open Access Journals (Sweden)

    Zoltán Noszticzius

    Full Text Available BACKGROUND / AIMS: ClO2, the so-called "ideal biocide", could also be applied as an antiseptic if it was understood why the solution killing microbes rapidly does not cause any harm to humans or to animals. Our aim was to find the source of that selectivity by studying its reaction-diffusion mechanism both theoretically and experimentally. METHODS: ClO2 permeation measurements through protein membranes were performed and the time delay of ClO2 transport due to reaction and diffusion was determined. To calculate ClO2 penetration depths and estimate bacterial killing times, approximate solutions of the reaction-diffusion equation were derived. In these calculations evaporation rates of ClO2 were also measured and taken into account. RESULTS: The rate law of the reaction-diffusion model predicts that the killing time is proportional to the square of the characteristic size (e.g. diameter of a body, thus, small ones will be killed extremely fast. For example, the killing time for a bacterium is on the order of milliseconds in a 300 ppm ClO2 solution. Thus, a few minutes of contact time (limited by the volatility of ClO2 is quite enough to kill all bacteria, but short enough to keep ClO2 penetration into the living tissues of a greater organism safely below 0.1 mm, minimizing cytotoxic effects when applying it as an antiseptic. Additional properties of ClO2, advantageous for an antiseptic, are also discussed. Most importantly, that bacteria are not able to develop resistance against ClO2 as it reacts with biological thiols which play a vital role in all living organisms. CONCLUSION: Selectivity of ClO2 between humans and bacteria is based not on their different biochemistry, but on their different size. We hope initiating clinical applications of this promising local antiseptic.

  3. Drug: D05142 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available Chemical (ATC) classification [BR:br08303] A ALIMENTARY TRACT AND METABOLISM A01 STOMATOLOGICAL PREPARATIO...NS A01A STOMATOLOGICAL PREPARATIONS A01AB Antiinfectives and antiseptics for local ... RESPIRATORY SYSTEM R02 THROAT PREPARATIONS R02A THROAT PREPARATIONS R02AB Antibi...) S03 OPHTHALMOLOGICAL AND OTOLOGICAL PREPARATIONS S03A ANTIINFECTIVES S03AA Antiinfectives S03AA01 Neomycin

  4. Essential oils used in aromatherapy: A systemic review

    OpenAIRE

    Babar Ali; Naser Ali Al-Wabel; Saiba Shams; Aftab Ahamad; Shah Alam Khan; Firoz Anwar

    2015-01-01

    Nowadays, use of alternative and complementary therapies with mainstream medicine has gained the momentum. Aromatherapy is one of the complementary therapies which use essential oils as the major therapeutic agents to treat several diseases. The essential or volatile oils are extracted from the flowers, barks, stem, leaves, roots, fruits and other parts of the plant by various methods. It came into existence after the scientists deciphered the antiseptic and skin permeability properties of es...

  5. Tablets with thyme (Thymus Vulgaris L) extracts

    OpenAIRE

    Zeković Zoran P.; Lepojević Žika D.; Markov Siniša L.; Milošević Svetlana G.

    2002-01-01

    The strongly antiseptic and antifungal activities of thyme is mainly due to the presence of phenolic compounds, thymol and carvacrol. Thyme extracts essential oil, extract obtained using 70% ethanol and extract obtained by supercritical fluid extraction (SFE) using carbon dioxide, were incorporated in the most useful form of pharmaceutical products - tablets. The work is concerned with the characterisation of the obtained tablets containing thyme extracts.

  6. [Labor epidural analgesia for a woman with a pityriasis versicolor in the lumbar region].

    Science.gov (United States)

    Dubar, G; Omarjee, M; Viguié, C; Barbarot, S; Mignon, A

    2011-01-01

    Epidural analgesia is usually contraindicated in case of infection at the site of needle insertion. Tinea versicolor is a benign superficial cutaneous fungal infection caused by the proliferation of a skin commensal yeast of low pathogenicity. We report the case of a pregnant woman with a tinea versicolor in the lumbar region, who benefited from a labor epidural analgesia, realised with reinforced antiseptic measures. No neurological or infectious complication occurred.

  7. Microbicide activity of clove essential oil (Eugenia caryophyllata)

    OpenAIRE

    Nuñez, L.; M.D. Aquino

    2012-01-01

    Clove essential oil, used as an antiseptic in oral infections, inhibits Gram-negative and Gram-positive bacteria as well as yeast. The influence of clove essential oil concentration, temperature and organic matter, in the antimicrobial activity of clove essential oil, was studied in this paper, through the determination of bacterial death kinetics. Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were the microorganisms selected for a biological test. To determine the temper...

  8. Composite Scaffolds Based on Silver Nanoparticles for Biomedical Applications

    OpenAIRE

    Jenel Marian Patrascu; Ioan Avram Nedelcu; Maria Sonmez; Denisa Ficai; Anton Ficai; Bogdan Stefan Vasile; Camelia Ungureanu; Madalina Georgiana Albu; Bogdan Andor; Ecaterina Andronescu; Laura Cristina Rusu

    2015-01-01

    This paper presents the synthesis, characterisation, and in vitro testing of homogenous and heterogeneous materials containing silver nanoparticles (nanoAg). Three types of antiseptic materials based on collagen (COLL), hydroxyapatite (HA), and collagen/hydroxyapatite (COLL/HA) composite materials were obtained. The synthesis of silver nanoparticles was realized by chemical reaction as well as plasma sputtering deposition. The use of chemical reduction allows the synthesis of homogenous mater...

  9. Stimulatory effect of Eucalyptus essential oil on innate cell-mediated immune response

    OpenAIRE

    Rasi Guido; Federici Memmo; Mercuri Luana; Zonfrillo Manuela; Andreola Federica; Vallebona Paola; Serafino Annalucia; Garaci Enrico; Pierimarchi Pasquale

    2008-01-01

    Abstract Background Besides few data concerning the antiseptic properties against a range of microbial agents and the anti-inflammatory potential both in vitro and in vivo, little is known about the influence of Eucalyptus oil (EO) extract on the monocytic/macrophagic system, one of the primary cellular effectors of the immune response against pathogen attacks. The activities of this natural extract have mainly been recognized through clinical experience, but there have been relatively little...

  10. PHARMACOGNOSTIC, PHYTOCHEMICAL AND PHYSICOCHEMICAL STUDIES OF PIPER NIGRUM LINN. FRUIT (PIPERACEAE)

    OpenAIRE

    P.V. Kadam; K.N. Yadav; F.A. Patel; F.A. Karjikar; M J Patil

    2013-01-01

    Piper species are reported to have great medicinal value in Indian medicine. Fruits of Piper nigrum Linn is commonly known as “Kalimiri” belongs to the family of Piperaceae and widely used as a pungent condiment. Traditionally it is used as antipyretic, diuretic, aphrodisiac, immune-stimulant, antioxidant, hepatoprotective, digestive, rubefacient, counter irritant, antiseptic, antispasmodic agent. Present work is related to standardization of Piper nigrum by using Pharmacognostic (Macroscopy,...

  11. Evaluation of in vitro antioxidant activity and free radical scavenging potential of variety of Tagetes erecta L. flowers growing in Bulgaria

    OpenAIRE

    Miglena Valyova; Stanimir Stoyanov; Yuliana Markovska; Yordanka Ganeva

    2012-01-01

    Summary. Marigold (Tagetes erecta L.) is well known for its antimicrobial, antiseptic, wound and ulcer healing, antiinflammatory, antioxidant and antiviral properties, and it has a long history of being used as an herbal remedy. T. erecta L. produces a variety of substances that possess pharmacological effects and antioxidant activity. The present study was therefore aimed to analyze the antioxidant activity of extracts and fractions of T. erecta L. flowers, cultivated in Bulgaria. Radical sc...

  12. The Local Treatment of Burns With Antibiotics

    OpenAIRE

    Napoli, B.; D’Arpa, N.; Masellis, A.; Masellis, M.

    2005-01-01

    After presenting an analysis of the principal antiseptics used for the local treatment of burns, highlighting their toxicity and the limitations of their antibacterial effectiveness, we describe the therapeutic protocol used in our burns centre (where antibacterial treatment consists exclusively of antibiotics for both local and systemic use). We review the data regarding actual and predicted mortality, and mortality due to septicaemia during the years 2000-2003.

  13. Slow Release of Nitric Oxide from Charged Catheters and Its Effect on Biofilm Formation by Escherichia coli▿

    OpenAIRE

    Regev-Shoshani, Gilly; Ko, Mary; Miller, Chris; Av-Gay, Yossef

    2009-01-01

    Catheter-associated urinary tract infection is the most prevalent cause of nosocomial infections. Bacteria associated with biofilm formation play a key role in the morbidity and pathogenesis of these infections. Nitric oxide (NO) is a naturally produced free radical with proven bactericidal effect. In this study, Foley urinary catheters were impregnated with gaseous NO. The catheters demonstrated slow release of nitric oxide over a 14-day period. The charged catheters were rendered antiseptic...

  14. Skin protection creams in medical settings: successful or evil ?

    OpenAIRE

    Charlier Corinne; Denooz Raphaël; Macarenko Elena; Xhauflaire-Uhoda Emmanuelle; Piérard Gérald E

    2008-01-01

    Abstract Background Chronic exposure to mild irritants including cleansing and antiseptic products used for hand hygiene generates insults to the skin. To avoid unpleasant reactions, skin protection creams are commonly employed, but some fail to afford protection against a variety of xenobiotics. In this study, two skin protection creams were assayed comparatively looking for a protective effect if any against a liquid soap and an alcohol-based gel designed for hand hygiene in medical setting...

  15. Presence of emerging contaminants priority substances and heavy metals in treated wastewater and groundwater in the Llobregat delta area (Barcelona, Spain)

    OpenAIRE

    Teijón Ávila, Gloria; Tamoh, Karim; Candela Lledó, Lucila

    2011-01-01

    Initial sampling results of emerging contaminants at the Llobregat delta deep aquifer (Barcelona, Spain) are presented. Among micro contaminants selected are 82 pharmaceuticals (antibiotics, lipid regulation agents, analgesic/anti-inflammatory drugs, etc), 18 personal care products (sunscreen agents, preservatives and disinfectants/antiseptics), 4 metals, 44 pesticides, 6 volatile priority pollutants, 16 PAHS and dioxins. Monitoring programme consisted on periodical water sampling from mun...

  16. Study on Patients Who Underwent Suspected Diagnosis of Allergy to Amide-Type Local Anesthetic Agents by the Leukocyte Migration Test

    Directory of Open Access Journals (Sweden)

    Mikio Saito

    2014-01-01

    Conclusions:: There is a high possibility that these adverse reactions were caused by pseudoallergy to drug. Even by allergic reactions, it was assumed that 80% of them might be caused by antiseptic agents such as paraben. In addition, it was suggested that ALAs, especially lidocaine hydrochloride preparations have high antigenicity (sensitizing property. Furthermore, it was considered that patients with past history of drug or food allergies have a high potential for manifestation of the reactions.

  17. Effect of Different Levels of Drought Stress (PEG 6000 Concentrations) On Seed Germination and Inorganic Elements Content in Purslane (Portulaca oleraceae L.) Leaves

    OpenAIRE

    Parvaneh Rahdari; Seyed Meysam Hoseini

    2012-01-01

    One of the most important environmental stresses is drought that great effect on plant grow and metabolism. In this study, drought stress effect on germination and inorganic elements content in leaves of medical plant Purslane (Portulaca oleraceae L.) were study. This plant as an Antiseptic, Antispasmodic, Diuretic, and Blood Filtering has medical application. Drought stress imposed by polyethylene glycol 6000 on seedlings and experiments in completely random model framework and with 3 replic...

  18. Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): A Review of Their Traditional Uses, Phytochemistry, and Pharmacology

    OpenAIRE

    Mahbubeh Bozorgi; Zahra Memariani; Masumeh Mobli; Mohammad Hossein Salehi Surmaghi; Mohammad Reza Shams-Ardekani; Roja Rahimi

    2013-01-01

    Pistacia, a genus of flowering plants from the family Anacardiaceae, contains about twenty species, among them five are more popular including P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus. Different parts of these species have been used in traditional medicine for various purposes like tonic, aphrodisiac, antiseptic, antihypertensive and management of dental, gastrointestinal, liver, urinary tract, and respiratory tract disorders. Scientific findings also revealed the w...

  19. [Health problems of combatants during the First World War].

    Science.gov (United States)

    Lefort, Hugues; Ferrandis, Jean-Jacques; Tabbagh, Xavier; Domanski, Laurent; Tourtier, Jean-Pierre

    2014-06-01

    The First World War because of the use of new weapons, injured more than 3 500 000 people (500 000 in the face), more than diseases (tuberculosis, typhoid fever, etc.) or even weather circumstances. The healing of the war wounds through surgery undertook a significant evolution thanks to the use of asepsis and antiseptics. Mortality go down, opening the way to the physical and psychological rehabilitation of those injured by the war.

  20. The percutaneous permeation of a combination of 0.1% octenidine dihydrochloride and 2% 2-phenoxyethanol (octenisept®) through skin of different species in vitro

    OpenAIRE

    Kietzmann Manfred; Siebert Joerg; Braun Michael; Stahl Jessica

    2011-01-01

    Abstract Background A water based combination of 0.1% octenidine dihydrochloride and 2% 2 - phenoxyethanol is registered in many European countries as an antiseptic solution (octenisept®) for topical treatment with high antimicrobial activity for human use, but octenidine based products have not been registered for veterinary use yet. The aim of the present study was to investigate whether octenidine dihydrochloride or 2 -phenoxyethanol, the two main components of this disinfectant, permeate ...

  1. Non-Surgical Chemotherapeutic Treatment Strategies for the Management of Periodontal Diseases

    OpenAIRE

    Krayer, Joe W.; Leite, Renata S.; Kirkwood, Keith L.

    2010-01-01

    Periodontal diseases are initiated by subgingival periodontal pathogens in susceptible periodontal sites. The host immune response towards periodontal pathogens helps to sustain periodontal disease and eventual alveolar bone loss. Numerous adjunctive therapeutic strategies have evolved to manage periodontal diseases. Systemic and local antibiotics, antiseptics, and past and future host immune modulatory agents are reviewed and discussed to facilitate the dental practitioner’s appreciation of ...

  2. Growth factors, silver dressings and negative pressure wound therapy in the management of hard-to-heal postoperative wounds in obstetrics and gynecology: a review

    OpenAIRE

    Stanirowski, Paweł Jan; Wnuk, Anna; Cendrowski, Krzysztof; Sawicki, Włodzimierz

    2015-01-01

    Purpose The last two decades witnessed the development of numerous innovative regimens for the management of patients with abnormally healing and infected wounds. Growth factors, negative pressure wound therapy (NPWT) and antiseptic dressings containing silver are examples of methods with best documented efficacy, being widely used in the treatment of acute and chronic post-traumatic wounds, burns and ulcers of various etiology. As far as obstetrics and gynecology are concerned, prevention an...

  3. Ultraviolet-B Protective Effect of Flavonoids from Eugenia caryophylata on Human Dermal Fibroblast Cells

    OpenAIRE

    Patwardhan, Juilee; Bhatt, Purvi

    2015-01-01

    Background: The exposure of skin to ultraviolet-B (UV-B) radiations leads to deoxyribonucleic acid (DNA) damage and can induce production of free radicals which imbalance the redox status of the cell and lead to increased oxidative stress. Clove has been traditionally used for its analgesic, anti-inflammatory, anti-microbial, anti-viral, and antiseptic effects. Objective: To evaluate the UV-B protective activity of flavonoids from Eugenia caryophylata (clove) buds on human dermal fibroblast c...

  4. Anti-inflammatory and antinociceptive activities A of eugenol essential oil in experimental animal models

    OpenAIRE

    Apparecido N. Daniel; Sartoretto, Simone M.; Gustavo Schmidt; Caparroz-Assef, Silvana M.; Ciomar A. Bersani-Amado; Roberto Kenji N. Cuman

    2009-01-01

    Eugenia caryophyllata, popular name "clove", is grown naturally in Indonesia and cultivated in many parts of the world, including Brazil. Clove is used in cooking, food processing, pharmacy; perfumery, cosmetics and the clove oil (eugenol) have been used in folk medicine for manifold conditions include use in dental care, as an antiseptic and analgesic. The objective of this study was evaluated the anti-inflammatory and antinociceptive activity of eugenol used for dentistry purposes following...

  5. Anti-cholinesterase activity of the standardized extract of Syzygium aromaticum L.

    OpenAIRE

    Manoj K. Dalai; Santanu Bhadra; Sushil K Chaudhary; Arun Bandyopadhyay; Mukherjee, Pulok K.

    2014-01-01

    Background: Clove (Syzygium aromaticum) is a well-known culinary spice with strong aroma; contains a high amount of oil known as clove oil. The major phyto-constituent of the clove oil is eugenol. Clove and its oil possess various medicinal uses in indigenous medicine as an antiseptic, anti-oxidant, analgesic and neuroprotective properties. Thus, it draws much attention among researchers from pharmaceutical, food and cosmetic industries. Objective: The aim of the present study was to determin...

  6. Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins

    OpenAIRE

    Hassan, Karl A.; Jackson, Scott M; Penesyan, Anahit; Patching, Simon G.; Tetu, Sasha G.; Eijkelkamp, Bart A.; Brown, Melissa H.; Henderson, Peter J. F.; Paulsen, Ian T.

    2013-01-01

    Drug resistance is an increasing problem in clinical settings with some bacterial pathogens now resistant to virtually all available drugs. Chlorhexidine is a commonly used antiseptic and disinfectant in hospital environments, and there is increasing resistance to chlorhexidine seen in some pathogenic bacteria, such as Acinetobacter baumannii. This paper examines the global gene expression of A. baumannii in response to chlorhexidine exposure and identifies a gene that we demonstrate to media...

  7. Chlorhexidine resistance in methicillin-resistant Staphylococcus aureus or just an elevated MIC? An in vitro and in vivo assessment.

    OpenAIRE

    Cookson, B D; Bolton, M C; Platt, J H

    1991-01-01

    Chlorhexidine (Hibiscrub; ICI) is generally accepted to be effective as an antiseptic hand wash for methicillin-susceptible Staphylococcus aureus (MSSA), but there is dispute whether the chlorhexidine MIC for methicillin-resistant S. aureus (MRSA) strains is higher than that for MSSA strains and, indeed, whether it is relevant. In addition, the link between resistance to chlorhexidine, gentamicin, and "nucleic acid-binding" compounds (NAB; which code, in particular, for propamidine isethionat...

  8. Chlorhexidine urticaria: A rare occurrence with a common mouthwash

    OpenAIRE

    Sharma Anamika; Chopra Harneet

    2009-01-01

    Chlorhexidine is a widely used antiseptic and disinfectant in medical and nonmedical environments. Compared to its ubiquitous use, allergic contact dermatitis from chlorhexidine has rarely been reported and so its sensitization rate seems to be low. The prevalence of contact urticaria and anaphylaxis due to chlorhexidine remains to be unknown. This case report presents a case of urticaria due to oral use of chlorhexidine. The adverse reaction was confirmed by skin prick test.

  9. Percutaneous absorption of chlorhexidine in neonatal cord care.

    OpenAIRE

    Aggett, P J; Cooper, L. V.; Ellis, S H; McAinsh, J

    1981-01-01

    The percutaneous absorption of chlorhexidine during its routine use in topical antiseptic preparations used in umbilical cord care was investigated by determining plasma chlorhexidine concentrations at ages 5 and 9 days. These showed that percutaneous absorption of chlorhexidine occurred in preterm neonates treated with a 1% solution of chlorhexidine in ethanol, but not in term infants similarly treated, or in preterm infants treated only with a dusting powder containing 1% chlorhexidine and ...

  10. An investigation into the role of dendrimers as potential enhancers of the dermal delivery of topically applied chlorhexidine

    OpenAIRE

    Judd, Amy Maryanne

    2013-01-01

    The reduction of bacteria on the skin results in prophylactic and therapeutic benefits by reducing the occurrence of skin infections. Currently, chlorhexidine digluconate, a conventional topical antiseptic, permeates the skin poorly leaving viable opportunistic pathogens below the superficial layers of the stratum corneum. The aim of this study was to use polyamidoamine (PAMAM) dendrimers to enhance the topical delivery of chlorhexidine digluconate to improve its antimicrobial efficacy.

  11. Chlorhexidine, A Medicine for all the Oral Diseases

    OpenAIRE

    Radhika Gupta; Vidya Chandavarkar; Sushama R Galgali; Mithilesh Mishra

    2012-01-01

    Chlorhexidine is a bisbiguanide antiseptic. It is active against both Gram-positive and Gram-negative strains as well as fungi. It has bacteriostatic and bactericidal actions. Chlorhexidine has excellent antiplaque activity and unique property of substantivity. So it has got wide applications starting from maintaining oral hygiene pre surgically to post operative and also in physically and mentally handicapped patients. Chlorhexidine is now routinely used by clinicians when they treat patient...

  12. Preoperative skin preparation with 2% chlorhexidine as a factor in the prevention of surgical site infection

    OpenAIRE

    Evelyn Solano Castro

    2014-01-01

    The results of secondary research that refers to preoperative skin preparation with antiseptic chlorhexidine 2% are presented. Surgical Site Infections are one of the most common complications in surgical procedures are associated with significant morbidity and mortality in the user and are the third -associated infection more frequent in the health care . Steps of clinical practice based on evidence were applied, considering in the first instance a question in PICO format, then a search for ...

  13. Wirkung einer Mundspülung mit Teebaumöl auf Plaque und Entzündung

    OpenAIRE

    Saxer, U P; Stäuble, A; Szabo, S H; Menghini, G

    2003-01-01

    English: The tea tree oil (melaleuca alternifolia) has antiseptic, fungicide and bactericide effects. The efficiency against oral bacteria was also evident. Xylitol is known for counterattacking the cariogenic effect caused by the streptococcus mutans. Less plaque was developed during the time of the study. Deutsch: Teebaumöl (Melaleuca alternifolia)verfügt über antiseptische, fungizide und bakterizide Wirkungen, welche auch gegen orale Keime nachgewiesen wurden. Xylitol ist bekannt als Z...

  14. Chemical composition and antimicrobial activity of peppermint (Mentha piperita L.) Essential oil

    OpenAIRE

    Mohaddese Mahboubi; Nastaran Kazempour

    2014-01-01

    Peppermint with antiseptic and known healing properties is a plant from the Labiatae family. In this study, we analyzed the chemical composition of essential oil from the flowering aerial part of peppermint by GC and GC/MS. Its antimicrobial activity was evaluated against bacteria, fungi and yeast by micro broth dilution assay. The fractional inhibitory concentration (FIC) and FIC Index (FICI) and related isobologram curve were determined by check board micro titer assay. The results...

  15. Chemical Composition and Antibacterial Activity of the Essence oil of Peppermint (Mentha piperita L)

    OpenAIRE

    Z Izadi; M Esna-Ashari; G Ahmadvand; P. Davoodi; KH Piri

    2009-01-01

    Introduction & Objective: Peppermint (Mentha piperita L.) is a perennial herbaceous essence oil bearing plant which belongs to the Lamiaceae family. This plant is a valuable and important herb which has many therapeutic properties. Recent investigations have shown its excellent anti-irritable bowel syndrome effects. Other properties of this plant are anti-inflammatory, analgesic, promote menstrual flow, antipyretic, antiseptic and anti-rheumatoid effects. This investigation was conducted to s...

  16. «KING OF PROBIOTICS» BACILLUS COAGULANS IN MODERN COMBINED PROBIOTIC PREPARATIONS LAKTOVIT FORTE (FULL REVIEW)

    OpenAIRE

    Bomko TV; Martynov AV; Nosalska TN; Каблучко ТВ

    2016-01-01

    Bacillus coagulans has an advantage over most other bacteria used as probiotics. It occupies an intermediate position between the genera Bacillusand Lactobacillus, is a spore-forming bacteria that produce lactic acid.This bacteria in the spores form can tolerate well technology processes, resistant to antibiotics and antiseptics, does not collapse under the influence of gastric juice and bile. Getting into the duodenum, the spores germinate into vegetative forms and begin vegetation and grow...

  17. Antibacterial Targets in Fatty Acid Biosynthesis

    OpenAIRE

    Wright, H. Tonie; Reynolds, Kevin A.

    2007-01-01

    The fatty acid biosynthesis pathway is an attractive but still largely unexploited target for development of new anti-bacterial agents. The extended use of the anti-tuberculosis drug isoniazid and the antiseptic triclosan, which are inhibitors of fatty acid biosynthesis, validates this pathway as a target for anti-bacterial development. Differences in subcellular organization of the bacterial and eukaryotic multi-enzyme fatty acid synthase systems offer the prospect of inhibitors with host vs...

  18. Drug: D03454 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D03454 Drug Cetrimonium bromide (NF/INN) C19H42N. Br 363.2501 364.4475 D03454.gif S...ium compounds D08AJ02 Cetrimonium D03454 Cetrimonium bromide (NF/INN) R RESPIRATO...RY SYSTEM R02 THROAT PREPARATIONS R02A THROAT PREPARATIONS R02AA Antiseptics R02AA17 Cetrimonium D03454 Cetrimonium bromide

  19. A REVIEW ON KRISHNA TULSI, OCIMUM TENUIFLORUM LINN.

    OpenAIRE

    Palla Ravi; A. Elumalai; M. Chinna Eswaraiah; Raju Kasarla

    2012-01-01

    Ocimum tenuiflorum is the most sacred herb in India and it is otherwise called as Krishna tulsi. O.tenuiflorum belongs to Lamiaceae family, which posses various healing medicinal properties for human life. Traditionally the various parts like leaves, flowers and stems are being used in the treatment various disorders such as skin diseases, cold, cough, fever, vomiting, swelling etc. Into this, O.tenuiflorum was reported to have anti-cancer, antimicrobial, antiseptic, antispasmodic, antifungal...

  20. Stimulation of proliferation and biocompatibility of polihexanide

    OpenAIRE

    C. Wiegand; Abel, M.; Kramer, A; Müller, G; Van Ruth, P.; Hipler, UC

    2007-01-01

    The therapy of critically colonized or locally infected chronic wounds often demands a antiseptic treatment to ensure the removal of pathogenic micro-organisms that could lead to an infection. Therefore, wound dressings combined with antimicrobial agents such as silver, povidone-iodine or polihexanide are increasingly utilized. Polihexanide is regarded first choice for chronic wounds because of its good skin and wound tolerance beside its antimicrobial effectivity. Furthermore, it possesses t...

  1. Wound Chemotherapy by the Use of Negative Pressure Wound Therapy and Infusion

    OpenAIRE

    Giovinco, Nicholas A.; Bui, Trung D.; Fisher, Timothy; Mills, Joseph L.; Armstrong, David G.

    2010-01-01

    Introduction: Although the use of negative pressure wound therapy (NPWT) is broadly efficacious, it may foster some potentially adverse complications. This is particularly true in patients with diabetes who have a wound colonized with aerobic organisms. Traditional antiseptics have been proven useful to combat such bacteria but require removal of some NPWT devices to be effective. Methods: In this article, we describe a method of “wound chemotherapy” by combining NPWT and a continuous infusio...

  2. Antioxidant Activities of Iranian Corn Silk

    OpenAIRE

    Ebrahimzadeh, Mohammad Ali; POURMORAD, Fereshteh; HAFEZI, Samira

    2008-01-01

    Traditionally corn silk (CS) has been used as diuretic, antilithiasic, uricosuric, and antiseptic. It is used for the treatment of edema as well as for cystitis, gout, kidney stones, nephritis, and prostatitis. In the present study, the antioxidant properties of ethanol-water extract from CS were estimated by different methods. Also phenol and flavonoid content of the extract were measured by Folin Ciocalteu and AlCl3 assays. CS extract contained a significant amount of phenol and flavonoids....

  3. Full-mouth treatment modalities (within 24 hours) for chronic periodontitis in adults

    OpenAIRE

    Eberhard, J.; Jepsen, S.; Jervøe-Storm, P. M.; Needleman, I.; Worthington, H. V.

    2015-01-01

    Periodontitis is chronic inflammation that causes damage to the soft tissues and bones supporting the teeth. Mild to moderate periodontitis affects up to 50% of adults. Conventional treatment is quadrant scaling and root planing. In an attempt to enhance treatment outcomes, alternative protocols for anti-infective periodontal therapy have been introduced: full-mouth scaling (FMS) and full-mouth disinfection (FMD), which is scaling plus use of an antiseptic. This review updates our previous re...

  4. The Clinical Effectiveness of Subgingival Irrigation with Povidone-Iodine and Hydrogen Peroxide in Treatment of Moderate to Severe Chronic Periodontitis

    OpenAIRE

    Khosropanah H.; Koohpeima F.; Ieal F.; Kiani Yazdi F.

    2012-01-01

    tatement of Problem: Although mechanical debridement is considered as the conventional technique in the management of chronic periodontitis, the locally delivered antiseptic agents have also been investigated as an adjunctive therapy. Purpose: The purpose of this study was to investigate the clinical effectiveness of subgingival irrigation with polyvinylpyrrolidone-Iodine (PVP-I) 10%, H2O2 3%, and the combination of both in the measurement of probing depth and plaque and gingival indices of p...

  5. Anti-Biofilm Compounds Derived from Marine Sponges

    OpenAIRE

    Christian Melander; Justin J. Richards; Richele Thompson; Ashley T. Tucker; Stowe, Sean D.; John Cavanagh

    2011-01-01

    Bacterial biofilms are surface-attached communities of microorganisms that are protected by an extracellular matrix of biomolecules. In the biofilm state, bacteria are significantly more resistant to external assault, including attack by antibiotics. In their native environment, bacterial biofilms underpin costly biofouling that wreaks havoc on shipping, utilities, and offshore industry. Within a host environment, they are insensitive to antiseptics and basic host immune responses. It is esti...

  6. Casbane diterpene as novel and natural antimicrobial agent against biofilm infections

    OpenAIRE

    Carneiro, Victor Alves; Teixeira, Edson Holanda; Santos, Hélcio Silva dos; M. O. Pereira; Henriques, Mariana; Lemos, Telma; Arruda, Francisco Vassiliepe Sousa

    2010-01-01

    Croton nepetaefolius is a plant native from northeastern Brazil and belongs to Euphorbiaceae family. The essential oil of this plant is widely used in folk medicine from the treatment of gastrointestinal disorders to the use as an antiseptic agent, with an antifungal action scientifically proven. The action of this plant has been extensively explored by the scientific community, being the secondary metabolites, which are responsible for their properties, alkaloids, diterpenes, and...

  7. Chemical composition and antioxidative activity of essential oil of Thymus serpyllum L.

    OpenAIRE

    Petrović Slobodan S.; Ristić Mihailo S.; Babović Nada V.; Lazić Miodrag L.; Francišković Marina; Petrović Slobodan D.

    2014-01-01

    Wild thyme (Thymus serpyllum L.) is a popular remedy regarding both traditional and conventional medicine. It is used as the antiseptic, aromatic, expectorant, stomachic, antispasmodic, carminative and preservative substance. For the purpose of this paper, wild thyme essential oil was isolated from the dried herb T. serpyllum by hydrodistillation. Original semi-industrial distillation device SP-130 performing distillation by water and steam was used for the...

  8. Rooting of Melaleuca alternifolia cuttings with different plant regulators
    Enraizamento de estacas de Melaleuca alternifolia submetidas a diferentes reguladores vegetais

    OpenAIRE

    Tereza Cristina de Carvalho; Luís Fernando Roveda; Marina Costacurta Antunes; Rosemeire Carvalho da Silva; Luiz Antonio Biasi

    2012-01-01

    The Melaleuca alternifolia is a Myrtaceae tree of great medicinal importance. Its essential oil is widely used both in human and in veterinary medicine, having great antiseptic power. It is a difficult plant in the process of rooting cuttings. This study aimed to evaluate the efficacy of different concentrations of NAA and IBA on the rooting of M. alternifolia. Melaleuca cuttings with 10 cm in length were submitted to treatments with two regulators, NAA and IBA, in the following concentration...

  9. The Styracaceae Styracaceae

    OpenAIRE

    Patrícia M. Pauletti; Helder L. Teles; Dulce H.S. Silva; Ângela R. Araújo; Vanderlan S. Bolzani

    2006-01-01

    The Styracaceae contains 11 genera and approximately 160 species consisting of small trees and shrubs, mostly native to tropical and subtropical regions. This family is well-known by the genus Styrax, which is notorious due to the production of resinous material, a pathological product, harvested by making incisions into the tree’s bark. The gum is used in perfumes, as antiseptic, expectorant, incense, and fumigating material. This paper reviews the phytochemical and biological studies carrie...

  10. Nephroprotective and hepatoprotective effects of Tribulus terrestris L. growing in Saudi Arabia

    OpenAIRE

    Maged S. Abdel-Kader; Abdullah Al-Qutaym­; Abdulaziz S. Bin Saeedan; Abubaker M. Hamad; Alkharfy, Khalid M

    2016-01-01

    Context: Tribulus terrestris (Zygophyllaceae) is a popular leafy prostrate branching herb used in folk medicine as a diuretic and urinary antiseptic. Aims: To evaluate the hepatoprotective and nephroprotective activities of the ethanolic plant extract and petroleum ether, dichloromethane and aqueous methanol fractions against CCl4 induced toxicity in adult Wistar rats. Methods: The total 95% ethanol extract at 200 and 400 mg/kg and petroleum ether, dichloromethane and aqueous methanol...

  11. Antisepsis of the Skin by Treatment with Tissue-Tolerable Plasma (TTP): Risk Assessment and Perspectives

    Science.gov (United States)

    Lademann, Jürgen; Richter, Heike; Patzelt, Alexa; Meinke, Martina C.; Fluhr, Joachim W.; Kramer, Axel; Weltmann, Klaus-Dieter; Lademann, Olaf

    The application of tissue tolerable plasma (TTP) is well suited for ­disinfection of living tissue. In particular, when treating chronic wounds, it has ­several advantages in comparison to the classical application of antiseptics, which do not penetrate sufficiently into the tissue or inhibit wound regeneration. The mode of action of the plasma is mainly based on synergetic effects between temperature increase and the formation of free radicals, which destroy the bacteria and fungi.

  12. 对提高我国酱油生产质量的探讨%Inquiring into how to improve the quality of soy sauce

    Institute of Scientific and Technical Information of China (English)

    杨纪红; 李文德

    2001-01-01

    本文主要在酱油原料处理方面介绍了一些新的技术,并在制曲,后期调配,防腐剂方面提出了一些探讨和建议。%This paper mainly gives some new informations on how to deal with the raw materials of soy and provides some suggestions on making leaven,mixing and antiseptics.

  13. A new surgical handwashing and hand antisepsis from scrubbing to rubbing.

    Science.gov (United States)

    Furukawa, Kiyonori; Ogawa, Rho; Norose, Yoshihiro; Tajiri, Takashi

    2004-06-01

    In 2002, the Centers for Disease Control and Prevention (CDC) published guidelines for surgical handwashing and hand antisepsis on the Internet. According to these guidelines, we revised our surgical handwashing method from scrubbing with brushes to rubbing with antiseptic. The new method consists of scrubbing around the nails with brushes and rubbing the hands and arms with antiseptic from the elbow to the antebrachium. A total of 182 surgeons and operating-room nurses participated in the current study. Bacterial contamination was investigated using the glove-juice method. The new surgical handwashing method is simple, and requires only a short time to perform (2 minutes 50 seconds). The bacterial examination confirmed that rubbing the hands with antiseptic was significantly more effective than scrubbing with brushes. In terms of sterilization or prolonged effects, 4% chlor-hexidine gluconate (CHG) was superior to 7.5% povidone-iodine (PVI) throughout a 3-hour period after hand antisepsis. Although bacterial counts were increased 3 hours after the beginning of surgery, additional hand rubbing with 0.2% chlorhexidine-83% ethanol (Hibisoft(TM)) was effective in suppressing the number of bacteria. Hibisoft(TM) successfully prolonged sterilization for more than 3 hours. For long surgical procedures, CHG should be used as an antiseptic and gloves should be changed every 3 hours, alcohol-based hand rubbing should also be performed 3 hours after the initial handwashing. This new technique will be included in the OSCE curriculum to ensure its standardization. Moreover, in-depth education regarding central operating-room practices is desired.

  14. Potassium permanganate ingestion as a suicide attempt

    OpenAIRE

    Tuba Cimilli Ozturk; Ozlem Guneysel; Onur Yesil; Sebnem Eren Cevik

    2012-01-01

    Potassium permanganate is a highly corrosive, water-soluble oxidizing antiseptic. A 68- year-old female patient was admitted to our Emergency Department after ingestion of 3 tablets of 250 mg potassium permanganate as a suicide attempt. The physical exam revealed brown stained lesions in the oropharynx. Emergency endoscopy was performed by the gastroenterologist after the third hour of ingestion. Emergency endoscopy revealed multiple superficial (Grade I-II) lesions on the esophagus and cardi...

  15. The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia

    OpenAIRE

    Mejdoub Hafedh; Bekir Ahmed; Ben Mansour Riadh; Ben Chobba Ines; Kadri Adel; Zarai Zied; Gharsallah Néji

    2011-01-01

    Abstract Background In order to validate its antiseptic and anticancer properties with respect to traditional uses, we have screened for the first time the antimicrobial activity of aerial parts of M. vulgare L. essential oil against different pathogenic microorganisms and the cytotoxic activity against HeLa cell lines. Methods The agar disk diffusion method was used to study the antibacterial activity of M. vulgare essential oil against 12 bacterial and 4 fungi strains. The disc diameters of...

  16. Co-operative inhibitory effects of hydrogen peroxide and iodine against bacterial and yeast species

    OpenAIRE

    Zubko, Elena I; Zubko, Mikhajlo K

    2013-01-01

    Background Hydrogen peroxide and iodine are powerful antimicrobials widely used as antiseptics and disinfectants. Their antimicrobial properties are known to be enhanced by combining them with other compounds. We studied co-operative inhibitory activities (synergism, additive effects and modes of growth inhibition) of hydrogen peroxide and iodine used concurrently against 3 bacterial and 16 yeast species. Results Synergistic or additive inhibitory effects were shown for hydrogen peroxide and ...

  17. p-Cresol Affects Reactive Oxygen Species Generation, Cell Cycle Arrest, Cytotoxicity and Inflammation/Atherosclerosis-Related Modulators Production in Endothelial Cells and Mononuclear Cells

    OpenAIRE

    Mei-Chi Chang; Hsiao-Hua Chang; Chiu-Po Chan; Sin-Yuet Yeung; Hsiang-Chi Hsien; Bor-Ru Lin; Chien-Yang Yeh; Wan-Yu Tseng; Shui-Kuan Tseng; Jiiang-Huei Jeng

    2014-01-01

    AIMS: Cresols are present in antiseptics, coal tar, some resins, pesticides, and industrial solvents. Cresol intoxication leads to hepatic injury due to coagulopathy as well as disturbance of hepatic circulation in fatal cases. Patients with uremia suffer from cardiovascular complications, such as atherosclerosis, thrombosis, hemolysis, and bleeding, which may be partly due to p-cresol toxicity and its effects on vascular endothelial and mononuclear cells. Given the role of reactive oxygen sp...

  18. The possibilities of using essential oils as an active ingredients or preservatives in cosmetic products

    OpenAIRE

    Adaszy?ska, Michalina; Swarcewicz, Maria

    2011-01-01

    An important trend in the development of the cosmetics industry is searching for new biologically active, natural compounds and preservative systems, which will find application in the natural cosmetics production. Natural cosmetics are of considerable interest nowadays and essential oils could be employed in theirs production. The huge potential of essential oils indicates the possibility of applying them in practice because of theirs antibacterial, antiseptic, antifungal, and antioxidant...

  19. BENEFITS OF HERBAL EXTRACTS IN COSMETICS: A REVIEW

    OpenAIRE

    Amreen Fatima*, Shashi Alok, Parul Agarwal, Prem Prakash Singh and Amita Verma

    2013-01-01

    Herbal extracts are primarily added to the cosmetic formulations due to several associated properties such as antioxidant, anti inflammatory, antiseptic and antimicrobial properties. Even today, people in rural and urban areas depend upon herbs for traditional cosmetics. Information on the herbal cosmetics was collected via electronic search (using pub med, scifinder, Google Scholar and web of science) and library search for articles published in peer-reviewed journals. Furthermore, informati...

  20. Hygienic requirements for changing wound dressings

    OpenAIRE

    Daeschlein, Georg; Chergui, Bettina; Kramer, Axel

    2006-01-01

    The purpose of changing wound dressings is to promote wound healing. To prevent wound infection or colonization with pathogens, hygienically correct wound care is necessary. Every change of dressing must be performed in accordance with aseptic or - if necessary - antiseptic principles. Following preparation of the dressing cart (or tray), the non-aseptic phase is performed, followed by the aseptic phase. The first rule of infection prevention is correct hand hygiene (hand disinfection, protec...

  1. In vitro antimicrobial activity of Pistacia lentiscus L. edible oil and phenolic extract.

    Science.gov (United States)

    Mezni, F; Aouadhi, C; Khouja, M L; Khaldi, A; Maaroufi, A

    2015-01-01

    Pistacia lentiscus L. is known in some Tunisian forest area by its fixed oil used in traditional medicine as an antiseptic product. This investigation is the first to study the antimicrobial activity of P.lentiscus edible oil and its phenolic extract. Oil was extracted from fruits harvested from six provenances located in Tunisia. The antimicrobial activity was tested using disc diffusion assay and the broth dilution method. Kbouch and Sidi Zid oils were most efficient (p oil and extract.

  2. The Effect of Different Levels of Altitutes on Composition and Content of Essential Oils of Ziziphora Clinopodioides in Southern of Turkey

    OpenAIRE

    MARAL, Hasan; Taghikhani, Hassan; KAYA, Alpaslan; Kirici, Saliha

    2015-01-01

    The genus Ziziphora L. belongs to the Lamiaceae family and consists of four species (Ziziphora clinopodioides, Ziziphora capitata, Ziziphora persica and Ziziphora tenuior). Z. clinopodioides is an edible medicinal plant that its leaves, flowers and stems are frequently used as wild vegetable or additive in foods to offer aroma and flavor.  The plant known locally as ‘Kır nanesi’ is used in the preparation of an aromatic tea for gastrointestinal disorders and as a carminative, antiseptic and w...

  3. Multilayer hydrogel coatings to combine hemocompatibility and antimicrobial activity.

    Science.gov (United States)

    Fischer, Marion; Vahdatzadeh, Maryam; Konradi, Rupert; Friedrichs, Jens; Maitz, Manfred F; Freudenberg, Uwe; Werner, Carsten

    2015-07-01

    While silver-loaded catheters are widely used to prevent early-onset catheter-related infections [1], long term antimicrobial protection of indwelling catheters remains to be achieved [2] and antiseptic functionalization of coatings often impairs their hemocompatibility characteristics. Therefore, this work aimed to capitalize on the antimicrobial properties of silver nanoparticles, incorporated in anticoagulant poly(ethylene glycol) (PEG)-heparin hydrogel coatings [3] on thermoplastic polyurethane materials. For prolonged antimicrobial activity, the silver-containing starPEG-heparin hydrogel layers were shielded with silver-free hydrogel layers of otherwise similar composition. The resulting multi-layered gel coatings showed long term antiseptic efficacy against Escherichia coli and Staphylococcus epidermidis strains in vitro, and similarly performed well when incubated with freshly drawn human whole blood with respect to hemolysis, platelet activation and plasmatic coagulation. The introduced hydrogel multilayer system thus offers a promising combination of hemocompatibility and long-term antiseptic capacity to meet an important clinical need.

  4. Effectiveness of ozone against periodontal pathogenic microorganisms.

    Science.gov (United States)

    Huth, Karin C; Quirling, Martina; Lenzke, Stefanie; Paschos, Ekaterini; Kamereck, Klaus; Brand, Korbinian; Hickel, Reinhard; Ilie, Nicoleta

    2011-06-01

    Ozone has been proposed as an adjunct antiseptic in periodontitis therapy. The aim of this study was to investigate the antimicrobial effectiveness of gaseous/aqueous ozone, in comparison with that of the established antiseptic chlorhexidine digluconate (CHX), against periodontal microorganisms. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Parvimonas micra in planktonic or biofilm cultures were exposed, for 1 min, to gaseous ozone, aqueous ozone, CHX, or phosphate-buffered saline (control). None of the agents was able to substantially reduce the A. actinomycetemcomitans count in biofilm cultures. In contrast, P. gingivalis, T. forsythia, and P. micra could be eliminated by 2% CHX or by ozone gas at 53 gm(-3) . Significantly greater antimicrobial effects were observed against planktonic cultures than against biofilm-associated bacteria. The rate of killing was influenced by the species of bacteria, and by the type and concentration of agent. There were no significant differences in the effectiveness of aqueous ozone (20 μg ml(-1) ) or gaseous ozone (≥ 4 gm(-3) ) compared with 2% CHX but they were more effective than 0.2% CHX. Therefore, high-concentrated gaseous and aqueous ozone merit further investigation as antiseptics in periodontitis therapy. A safe system for applying gaseous ozone into the periodontal pocket that avoids inhalation still needs to be developed.

  5. [The efficacy of three hand asepsis techniques using chlorhexidine gluconate (CHG 2%)].

    Science.gov (United States)

    da Cunha, Érika Rossetto; Matos, Fabiana Gonçalves de Oliveira Azevedo; da Silva, Adriana Maria; de Araújo, Eutália Aparecida Cândido; Ferreira, Karine Azevedo São Leão; Graziano, Kazuko Uchikawa

    2011-12-01

    The scrubbing of hands and forearms using antiseptic agents has been the standard pre-operative procedure to prevent surgical site infection. With the introduction of antiseptic agents, the need to use brushes for pre-operative disinfection has been questioned and it has been recommended that the procedure be abandoned due to the injuries it may cause to the skin. With the purpose to provide the foundations for the efficacy of pre-operative asepsis without using brushes or sponges, the objective of this study was to evaluate three methods of pre-operative asepsis using an antimicrobial agent containing chlorhexidine gluconate - CHG 2%; hand-scrubbing with brush (HSB), hand-scrubbing with sponge (HSS), and hand-rubbing with the antiseptic agent (HRA) only. A comparative crossover study was carried with 29 healthcare providers. Antimicrobial efficacy was measured using the glove-juice method before and after each tested method. Statistical analyses showed there were no significant differences regarding the number of colony-forming units when comparing HRA, HSB, and HSS techniques (p=0.148), which theoretically disregards the need to continue using brushes or sponges for hand asepsis.

  6. Mouthwashes for the control of supragingival biofilm and gingivitis in orthodontic patients: evidence-based recommendations for clinicians

    Directory of Open Access Journals (Sweden)

    Alex Nogueira HAAS

    2014-01-01

    Full Text Available Properly performed daily mechanical biofilm control is the most important prevention strategy for periodontal diseases. However, proper mechanical biofilm control is not performed effectively by the majority of the population, mainly due to lack of motivation and of manual dexterity. Local biofilm retention factors may aggravate home oral hygiene quality. For this reason, patients wearing fixed orthodontic appliances comprise a group that may benefit from the daily use of mouthwashes. The purpose of this review was to perform a systematic search in the literature on antiseptics used to control supragingival biofilm and gingivitis in orthodontic patients. Six studies investigating the effect of chlorhexidine and 5 studies evaluating the effect of the daily use of antiseptics were found. Chlorhexidine showed better results in reducing plaque and gingivitis. However, because of its adverse effects after continuous use, it should not be indicated for long-term periods. Among the agents considered for daily use, the fixed combination of essential oils was the only one evaluated in a clinical trial, in which a comparative group presented a statistically significant clinical impact. There is no direct evidence supporting the indication of antiseptic agents for orthodontic patients other than chlorhexidine and essential oils. It can be concluded that, for patients undergoing orthodontic treatment, chlorhexidine should be considered for treating acute gingival inflammation, whereas essential oils should be indicated for long-term daily use in controlling supragingival biofilm.

  7. Risk assessment of the application of a plasma jet in dermatology

    Science.gov (United States)

    Lademann, Juergen; Richter, Heike; Alborova, Alena; Humme, Daniel; Patzelt, Alexa; Kramer, Axel; Weltmann, Klaus-Dieter; Hartmann, Bernd; Ottomann, Christian; Fluhr, Joachim W.; Hinz, Peter; Hübner, Georg; Lademann, Olaf

    2009-09-01

    Regardless of the fact that several highly efficient antiseptics are commercially available, the antiseptic treatment of chronic wounds remains a problem. In the past, electrical plasma discharges have been frequently used in biometrical science for disinfection and sterilization of material surfaces. Plasma systems usually have a temperature of several hundred degrees. Recently, it was reported that ``cold'' plasma can be applied onto living tissue. In in vitro studies on cell culture, it could be demonstrated that this new plasma possesses excellent antiseptic properties. We perform a risk assessment concerning the in vivo application of a ``cold'' plasma jet on patients and volunteers. Two potential risk factors, UV radiation and temperature, are evaluated. We show that the UV radiation of the plasma in the used system is an order of magnitude lower than the minimal erythema dose, necessary to produce sunburn on the skin in vivo. Additionally, thermal damage of the tissue by the plasma can be excluded. The results of the risk assessment stimulate the in vivo application of the investigated plasma jet in the treatment of chronic wounds.

  8. 甜高粱茎秆保存方法与优化研究%RESEARCH ON SWEET SORGHUM STALKS STORAGE TECHNOLOGIES FOR FUEL ETHANOL PRODUCTION

    Institute of Scientific and Technical Information of China (English)

    韩冰; 李纪红; 李十中; 李天成

    2012-01-01

    The preservation of sugars in sweet sorghum stalks is one of she key blocks on the way to realize the industrialization of sweet sorghum talks as a feedstock of fuel ethanol. The "antiseptic and anaerobic" method for sweet sorghum stalks storage was proposed and validated. Two kinds of antiseptics (potassium sorbate and sodium dehydroacetate) were investigated during the anaerobic storage of sorghum stalks at different conditions. The sugar and water contents in sweet sorghum stalks can be maintained effectively by using "antiseptic-anaerobic" storage. The sugar loss rate is less than 5. 95% after 6 months, and the sugar conversion rate is above 93. 43%.%针对环境微生物的侵染是造成茎秆糖分损失的状况,提出“抑菌-厌氧”贮存法,考察厌氧条件下两种抑菌剂(山梨酸钾和脱氢醋酸钠)对不同气候条件甜高粱茎秆贮藏效果的影响.结果表明,最优的“抑菌-厌氧”贮存法6个月茎秆糖分损失率为5.95%,无需复水便可进行固态发酵,且乙醇发酵总糖转化率在93.43%以上.

  9. 麻辣风味香肠防霉措施研究%Study on mildew prevention measure of hot and spicy flavor sausage

    Institute of Scientific and Technical Information of China (English)

    王家国; 董镔; 周明光; 吴平

    2012-01-01

    研究了散装麻辣风味香肠(以下简称麻辣肠)加工工序防腐处理对保质期的影响,发现以下措施均可提高麻辣肠的保质期:用复配防腐液浸泡肠衣;对成品表面进行复配防腐液喷涂;在包装中添加干燥剂。采用综合措施生产的一批麻辣肠,产品放在0—4℃冷库存放16d,在蒸煮间存放20d产品没有发霉变质现象,达到了预期效果。%In this article, the effect of antiseptic treatment during producing process of hot and spicy flavor sausage on its shelf life was researched, and the results showed that all of the following treatments could prolong the product shelf life: soaking enteric with compound antiseptic liquid; Spraying compound antiseptic liquid on the surface of end -product; adding dry agent inside of the package. A batch of hot and spicy flavor sausage were produced with this synthetical measure, and the product showed no mildew or degeneration after 16d storage at 0 -4℃ or stored at steam boil room for 20d, which achieved the expect result.

  10. Lubricant for hot processing of metals

    Energy Technology Data Exchange (ETDEWEB)

    Forostyan, Yu.N.; D' yachenko, K.A.; Grudev, A.P.; Lobarev, M.I.; Sigalov, Yu.B.

    1980-04-30

    The compositon of lubricant for hot processing of metals is based on water, hydrolysis lignin, phosphoric acid salt and an antiseptic. In order to increase the wear resistance of the instrument, it contains Ca(OH)/sub 2/, a soap stock of vegetable oil (SRM), dichlorstearic acid (I), tetrachlorstearic acid (II) and as the phosphoric acid salt, the lubricant contains trisodium phosphate. The % composition of the content are: hydrolysis lignin, 5-25; trisodium phosphate, 1-5; Ca(OH)/sub 2/, 0-5; vegetable oil soap stock, 10-30; I, 0.5-2; II, 0.5-10; antiseptic, 0.01-5 and water to 100%. The calculated volumes of lignin, Ca(OH)/sub 2/ and water are ground in a ball mill to a powder dispersion of 10-20 mkm, after which the SRM and the trisodium phosphate are added and the griding is continued for 1.5-2 hours. The formed mixture is reloaded into the reactor, heated to 75-80/sup 0/C with mixing, I and II, preheated to 70-80/sup 0/C are introduced. The heating and mixing are continued until the formation of a uniform gel. After cooling, the antiseptic is added. The use of the lubricant provides for a reduction in the rolling pressures from 17.3 to 15.6-16.8 t.

  11. 大豆无菌苗的获得%Suggestion of Obtaining Aseptic Soybean Seedlings

    Institute of Scientific and Technical Information of China (English)

    邢少辰; 林秀峰; 庄炳昌

    2001-01-01

    采用升汞和次氯酸纳两种消毒剂对野生大豆和栽培大豆进行处理,获得大豆的无菌苗。结果表明:①野生大豆对消毒剂的抵抗能力明显高于栽培大豆;②用水浸泡的栽培大豆再用升汞和次氯酸纳处理时,都不能发芽或极少发芽,说明消毒剂对浸泡的栽培大豆伤害十分严重;③种子放置在固体培养基的表面生长较快。%To obtain aseptic soybean seedlings,wide soybean and cultivated soybean were asepticized using HgCl2 and NaClO.The results showed that the wide soybean performed much higer resistance to antiseptic than cultivatied soybean.Secondly,the soaked seeds of cultivatied soybean which were treated by antiseptic can't germinate or very few seeds able to germinate,that means the antiseptic hurted the seeds seriously.Thirdly,the seeds grow faster on the surface of solid MS medium than that the seeds in other positions.

  12. [What is the scientific basis of our surgical beliefs?].

    Science.gov (United States)

    Gorj, M; Revol, M

    2015-02-01

    The purpose of the study is to assess the validity of surgical beliefs and their application in the operating room… An exhaustive revue of literature was performed using PubMed and the Cochrane Library. We found over 400 articles, and chose to select the 135 most pertinent ones. Antiseptic hand washing and the use of sterile gloves are the main elements of SSI prevention. In this field, use of hydroalcoholic solutions and chlorhexidine seem to be the more efficient. Regarding the surgical gown, the use of masks and surgical hands contamination. Antiseptic pre-operative shower has no advantage over a simple soap shower. Also depilation has no influence. Regarding the surgery in itself, cutaneous preparation is more efficient with alcoholic antiseptics or with chlorhexidine. After the surgery, no wound dressing has shown to ease the healing when compared to the use of regular water. There are two types of attitudes in surgery: those that have a proven benefit for the patient and/or the surgeon and those based on good intentions or beliefs but that have no scientific ground.

  13. Clinical investigation of catheter-related infections in two central venous cathers%两种中心静脉导管相关性感染的临床观察

    Institute of Scientific and Technical Information of China (English)

    高立平

    2012-01-01

    目的 评价抗感染中心静脉导管在减少重症监护病房(ICU)患者导管相关性感染(CRI)中的作用.方法 将248例在ICU留置中心静脉导管的患者随机分成普通中心静脉导管组(对照组120例)和抗感染中心静脉导管组(抗感染组128例),观察两组CRI的发生率和病原菌分布情况.结果 抗感染组的CRI发生率明显低于对照组(6.3%vs14.2%),差异有统计学意义(P<0.05).CRI的病原菌为金黄色葡萄球菌、鲍曼不动杆菌、阴沟肠杆菌、肠球菌、肺炎克雷伯菌和白色念珠菌,但两组患者CRI病原菌分布比较,差异无统计学意义(P>0.05).结论 抗感染中心静脉导管可明显降低ICU患者CRI的发生率.%Objective To evaluate the effect of reducing intravenous catheter-ralated infection (CRT) using antiseptic impregnated central venous catheter in patients in intensive care unit (ICU). Methods 248 patients treated in ICU who needed intravascular catheterization were randomly divided into the control group (120 cases, treated with ordinary central venous catheter) and the antiseptic group (128 cases, treated with antiseptic impregnated central venous catheter). The incidence of CRI and pathogenic distribution were observed. Results The incidence of CRI in the antiseptic group was significantly lower than that of the control group (6.3% vs 14.2%, P<0.05). The pathogens of CRI were Staphytococcus aureus, Acmetabactor baumarwiii, E. Cloacae, entemcoccus, Klebsiella pneumonias and Candida albicans. Pathogenic distribution between the two groups showed no statistically significant difference (PX).O5). Conclusion Antiseptic impregnated central venous catheter can obviously reduce the incidence of CRI in patients in ICU.

  14. Genital antisepsis--test methodology and efficacy of povidone-iodine.

    Science.gov (United States)

    Wewalka, G; Kurz, C; Enzelsberger, H

    1993-01-01

    Indications for antiseptic prophylaxis include use prior to urinary catheterization and other transurethral instrumentations, diagnostic or therapeutic instrumentation of the cavum uteri, and operations on the external genitalia and the vagina. Indications for antiseptic therapy include treatment of wounds and of infections of the genital tract and also include treatment of the vaginas of pregnant women to prevent infection in the newborn. For prophylactic antisepsis the aim is the optimal reduction of potentially pathogenic microorganisms. In some fields of application, e.g. before urinary catheterization, an immediate effect on the mucous membrane is required, but on other occasions, such as transvaginal operations, an additional sustained effect is desired. Testing the efficacy of mucous membrane antiseptics, especially for the genital tract, necessitates the establishment of standardized test methods. The variability in vaginal flora at various ages makes it reasonable to study women aged 20-50 years. Studies presented in this paper indicate that microorganism sampling using a cotton swab moistened with neutralizing fluid can be favoured compared to using a rinsing technique and that anaerobic culture techniques enable the measurement of the high reduction factors achieved by very efficient antiseptic procedures. Test methods validated for hand disinfectants are used for calculations and statistical evaluation. The antimicrobial efficacy of six vaginal antiseptic procedures were compared in patients prior to vaginal surgery. Three minutes after treatment, the highest mean reduction (log RF) of the normal vaginal flora as well as of potentially pathogenic microorganisms was obtained by povidone-iodine solution undiluted and povidone-iodine solution 1:10 (log RF 3.60 and 2.68, respectively). Out of three detergents with antimicrobial efficacy, octenidine 0.1% was the most effective preparation (log RF 2.32). Chlorhexidine 0.1%, hexetidine 0.1% and chlorhexidine 0

  15. Comparison of Antiseptics’ Efficacy on Pseudomonas Aeruginosa, StaphylococcusEpidermidis and Enterobacter Aeruginosa in Hospital of Imam Khomeini (Urmia

    Directory of Open Access Journals (Sweden)

    Fahim Amini

    2012-04-01

    Full Text Available Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 Background and Objectives: Nosocomial infection is the cause of deaths, morbidity, higher costs and increased length of stay in hospitals. Correct and appropriate use of antiseptic and disinfectants play an important role in reducing infections. In this study the efficacy of antiseptics on bacteria causing hospital infections has been studied.Materials and Methods: This study was conducted in the laboratory of Imam Khomeini Hospital of Uremia. In this study the Antimicrobial activity of Descocid, Korsolex basic, Mikrobac forte and persidin 1% was studied against bacteria causing hospital infections such as Enterobacter aeruginosa 1221 (NCTC 10006, Staphylococcus epidermidis (PTCC: 1435 (Cip81.55 and Pseudomonas aeruginosa Strain PAO1. Sensitivities of bacteria were determined by Minimum inhibitory Concentration (MIC and Minimum bactericidal Concentration (MBC antiseptics. In the second stage, the concentration of antiseptics was prepared according to the manufacturer's suggested protocol and the effect of antimicrobial agents were studied at the certain concentration and contact time.Result: All disinfectants (Descocid, Korsolex basic, Mikrobac forte concentration and contact time, Accordance with the manufacturer's brochure, had inhibitory effect on all bacteria. That this is consistent with the manufacturer's brochure. Persidin one percent in concentration of from 2 and 4 V/V % and exposure time 5 minutes could not inhibit the growth of bacterial. But at concentrations of 10 and 20% respectively 15 and 30 minutes exposure time, all three types of bacteria can be inhibited, which is consistent with the manufacturer's claims.Conclusion: In this study, the efficacy of antiseptics was determined with the Micro-dilution method recommended by the NCCLS. Korsolex basic, weakest antiseptics (the highest MIC for the inhibition of three bacteria was determined

  16. CLINICAL EVALUATION OF PREOPERATIVE SKIN PREPARATION WITH AQUEOUS POVIDONE IODINE ONLY AND IN COMBINATION WITH ALCHOLIC CHLORHEXIDINE IN PATIENTS UNDERGOING ELECTIVE AND EMERGENCY SURGERIES

    Directory of Open Access Journals (Sweden)

    Latchu

    2015-10-01

    Full Text Available INTRODUCTION: Many techniques are there for skin preparation before surgery, the commonest being initial scrub with antiseptic soap solution, followed by painting the prepared area with antiseptic paint solution. But degerming of the skin can be done with antiseptics us ed for less than one minute which is as effective as five minute scrub with germicidal soap solution followed by painting with antiseptics . AIMS AND OBJECTIVES : 1. To evaluate the efficacy of povidone iodine alone and antiseptic agent containing alcoholic chlorhexidine with povidone iodine in preoperative skin preparation by taking swab culture. 2. To compare the rate of postoperative wound infection in both the groups . METHODS: STUDY DESIGN: Comparative study conducted on 100 patients in two groups. STUDY SETTING: Sri Venkateswara Medical College Tirupathi SOURCE OF DATA: 100 Patients (50 in each Group undergoing elective and emergency surgery admitted in the Department of General Surgery in S.V.R.R. Government General Hospital, Tirupati from 2013 to 2014. INCLUSION CRITERIA: 1. Patients undergoing elective & emergency surgery in department of general surgery. 2. Patients with no focus of infection anywhere on the body. 3. Patients irrespective of their age and sex. 4. Patients neither immunocompromised nor on any long term steroids. 5. Patients undergoing mes h repair of hernia are also included. EXCLUSION CRITERIA: 1. Immuno compromised patients and patients on long term steroids. 2. Patients with septicemia. 3. Patients suffering from malignancies or undergoing chemotherapy or radiation therapy. 4. Contaminat ed surgeries in which viscus was opened were excluded from the study. 5. Patients with co - morbid medical conditions like diabetes, hypertension etc. METHOD OF COLLECTION OF DATA: In each case preoperatively, detailed history was taken and routine investiga tions like haemoglobin, total count, differential count, ESR, RBS and chest X - ray, ultrasound were done to

  17. ESTIMATION OF THE EFFECTIVENESS OF DECASAN, DECAMETHOXIN AND ITS COMPOSITION USAGE IN PATIENTS WITH SEVERE THERMAL INJURY

    Directory of Open Access Journals (Sweden)

    Nazarchuk O.А.

    2015-05-01

    Full Text Available Introduction. Nowadays victims with burn trauma are one of the most important categories of patients in the emergent surgery. According to the data of WHO burns happen in 5,6 – 10 % of cases among all kinds of trauma. Purulentinflammatory complications in these patients are of great importance.The aim was to study microbiological, clinical effectiveness of antiseptics, antimicrobial composition of decamethoxin with modified polysaccharides, antimicrobial materials in prophylaxis and treatment of infectious complications in patients with burn injury. Materials and methods. In the research microbiological study of antimicrobial activity of modern antiseptics, antimicrobial materials against opportunistic pathogens of purulent-inflammatory complications in patients with difficult burn injury and clinical observation of effectiveness of the use of antimicrobial composition (AMC of decamethoxin (DKM with carboxymethylamylum, oxyethylcellulose, polyvinilacetate. There were 130 patient with difficult burn injury (the 3rd- 4th stages; injury square – 10,0 – 85,0 % of surface enrolled in the study. All patients underwent early surgery on the 2nd- 3rd day after trauma. Complex intensive care was provided to every patient. Microbiological examinations of patients (100 % were carried out before antibacterial treatment and every 7 days during treatment. Antimicrobial qualities of antiseptics (decasan, miramistin, chlorhexidine digluconate and AMC against S. aureus (n 35, S. epidermidis (n 12, Enterococcus spp. (n 9, P. aeruginosa (n 39, A. baumannii (n 54, Proteus spp. (n 16, Enterobacter spp. (n 11, K. pneumoniae (n 12, E. coli (n 9, C. albicans (n 7 were studied according to standard methods. Antimicrobial qualities of dressings, containing antiseptics we studied on clinical strains of S. aureus, E. coli, P. aeruginosa on dense medium, counting diameter of growth delay zones (mm. Results and discussion. Results of study of sensitivity of Gram

  18. Comparison of the antibacterial activity of an ozonated oil with chlorhexidine digluconate and povidone-iodine. A disk diffusion test.

    Science.gov (United States)

    Montevecchi, Marco; Dorigo, Antonio; Cricca, Monica; Checchi, Luigi

    2013-07-01

    Ozonated oils are antiseptics obtained from the chemical reaction between ozone and unsaturated fatty acids of vegetable oils. The aim of this study was to investigate the antimicrobial effectiveness of a commercially available ozonated oil (O3-Oil), in comparison with 0.2% chlorhexidine digluconate (CHX) and 10% povidone-iodine (PVP-I) through a disk diffusion test. For each antiseptic a series of two-fold dilutions was made, obtaining seven dilutions: 1:2, 1:4, 1:8, 1:16, 1:32, 1:64 and 1:128. The undiluted antiseptics and the seven dilutions were tested against two freeze-dried bacterial strains: Staphylococcus aureus (Sa) and Porphyromonas gingivalis (Pg). O3-Oil showed significantly greater diameters of growth inhibition (p<0.01) than CHX and PVP-I in all dilutions for both tested strains. CHX lost any antibacterial efficacy when diluted more than 1:32. At the highest dilution, the diameters of growth inhibition against Sa were 20.67±0.58 mm and 15.33±0.58 mm, for O3-Oil and PVP-I, respectively. At the same dilution, the diameters of growth inhibition against Pg were: 19.00 mm for O3-Oil and 13.67±0.58 mm for PVP-I. The promising results obtained for the O3-Oil, against the opportunistic Sa, and Pg, one of the main periodontal pathogens, suggest its potential applicability for periodontal treatment. Further preclinical and clinical investigations are warranted.

  19. In vitro growth-inhibitory effect of ethanol GRAS plant and supercritical CO₂ hop extracts on planktonic cultures of oral pathogenic microorganisms.

    Science.gov (United States)

    Pilna, J; Vlkova, E; Krofta, K; Nesvadba, V; Rada, V; Kokoska, L

    2015-09-01

    Conventional chemical antiseptics used for treatment of oral infections often produce side-effects, which restrict their long-term use. Plants are considered as perspective sources of novel natural antiseptics. However, little is still known about their inhibitory properties against oral pathogens. The objective of this study was to test in vitro antimicrobial activities of generally recognized as safe (GRAS) species against planktonic cultures of cariogenic, periodontal and candidal microorganisms and identify active compounds of the most active extracts. Growth-inhibitory effects of ethanol extracts from 109 GRAS plant species, six Humulus lupulus cultivars and two hop supercritical CO2 extracts were evaluated using broth microdilution method. The chemical analysis was done through high-performance liquid chromatography. Best results were obtained for supercritical CO2 and ethanol extracts of H. lupulus with minimum inhibitory concentrations (MIC) ≥8 μg/mL and ≥16 μg/mL, respectively. The chemical analysis of supercritical CO2H. lupulus extracts revealed that α- and β-acids were their main constituents. Capsicum annuum and Capsicum frutescens showed antibacterial effect against Streptococcus sobrinus and Streptococcus salivarius (MIC=64-128 μg/mL). These strains were further inhibited by Zanthoxylum clava-herculis (MIC=64-128 μg/mL) and Myristica fragrans (both MIC≥128 μg/mL). The latter also exhibited antimicrobial activity against Fusobacterium nucleatum (MIC=64 μg/mL). Punica granatum possessed inhibitory effects against Candida albicans (MIC=128 μg/mL) and F. nucleatum (MIC=64 μg/mL). The results indicate that supercritical CO2H. lupulus extracts together with ethanol extracts of C. annuum, C. frutescens, M. fragrans, P. granatum and Z. clava-herculis are promising materials for further investigation on new antiseptic agents of oral care products. PMID:26232134

  20. Clinical implications of the growth-suppressive effects of chlorhexidine at low and high concentrations on human gingival fibroblasts and changes in morphology.

    Science.gov (United States)

    Wyganowska-Swiatkowska, Marzena; Kotwicka, Malgorzata; Urbaniak, Paulina; Nowak, Agnieszka; Skrzypczak-Jankun, Ewa; Jankun, Jerzy

    2016-06-01

    Chlorhexidine (CHX) is considered the gold standard in the antiseptic treatment of the oral cavity, due to its high antibactericidal capability. With the use of CHX mouth-rinse formulations, the bacteriostatic effects are maintained by the adsorption and prolonged release of CHX from oral surfaces. It was believed that antiplaque formation ability and the lack of systemic toxicity of CHX render it an excellent antiseptic in post-surgical dental treatment. However, recent studies have demonstrated that CHX exerts cytotoxic effects on human periodontal tissues, such as gingival fibroblasts and other cells. It also reduces gingival fibroblast adhesion to fibronectin and prevents fibroblast attachment to root surfaces, thus interfering with periodontal regeneration. In this study, using human gingival fibroblasts (HGFs), we investigated effects of CHX on the growth, morphology and proliferation of HGFs. We found that a low concentration (0.002%) of CHX does not interfere with the proliferation and morphology of HGFs. However, a higher concentration (≥0.04%) of CHX inhibits cell proliferation and to a certain extent, affects cell morphology in a time-dependent manner. A decrease in the percentage of cells in the G0/G1 phase and the accumulation of cells in the S phase following treatment with CHX also occurred in a dose-dependent manner. We thus concluded that CHX only at the concentration of 0.002% does not interfere with HGF growth, that is so critical to wound healing. Thus, the application of CHX in the post-surgical antiseptic treatment of the oral cavity should be limited. PMID:27082817

  1. 年糕中腐败霉菌的分离纯化与控制的研究%Isolation, purification and inhibition of the spoilage moulds in the rice cake

    Institute of Scientific and Technical Information of China (English)

    谢欣; 许喜林; 林加燕

    2012-01-01

    The moulds contamination of the rice cake on its transportation and storage in Guangdong province was studied. Four mould strains were gained after isolation and purification of the spoilage moulds in rice cake, namely Aspergillus, Penicillium and Trichothecium. Five types of inhibitors were used to inhibit the moulds from rice cake, and the results showed that sodium dehydroacetate has the best antiseptic effect, besides the natural antiseptic-cinnamon also showed good results. It would achieve a better antiseptic effect and extend the shelf life of rice cake if we combined the inhibitors of sodium dehydroacetate and cinnamon.%针对广东地区年糕在储藏、运输过程中易滋生霉菌等问题进行研究。从已发霉变质的年糕中经过分离、纯化得到4株霉菌,并进行初步鉴定,结果表明它们分别为曲霉菌(Aspergillus)、青霉菌(Penicillium)和单端孢霉(Trichothecium)。选取5种抑菌剂分别对分离出的4株霉菌进行抑菌试验,结果表明脱氢醋酸钠抑菌效果最佳,而作为天然防腐剂的肉桂精油也表现出较好的抑菌效果;若能将两者结合使用,则可达到抑菌、防腐和延长年糕保质期的目的。

  2. Comparative study of different treatment options of grade III and IV diabetic foot ulcers to reduce the incidence of amputations

    Directory of Open Access Journals (Sweden)

    Poras Chaudhary

    2013-02-01

    Full Text Available This study aims to compare the efficacy of antiseptic dressings, hyperbaric oxygen therapy, and recombinant human platelet derived growth factor (rhPDGF for two reasons: i to reduce the incidence of lower limb amputations in diabetic foot ulcer; ii to limit the duration of stay in the hospital. A prospective randomized trial was conducted on 60 patients with stage III and IV diabetic foot ulcers (International Association of Enterostomal Therapy classification and patients were divided randomly in three different therapy groups - antiseptics, hyperbaric oxygen therapy, recombinant platelet derived growth factor, with 20 patients in each group. Patients were managed initially on inpatient and then on outpatient basis till the ulcer healed completely. Results among three groups were compared using unpaired T test and the level of significance was set at P<0.05 using ANOVA. This study compares the efficacy of hyperbaric oxygen therapy, antiseptic dressings, and rhPDGF in grade III and IV diabetic foot ulcers. P value (0.0348 was significant for complete wound contraction while p value healing time (0.6534 and ulcer size (0.0593 in the groups was not significant. PDGF is safe, effective and easy to apply. Results are comparable with hyperbaric oxygen (HBO therapy and cost of treatment is lower than other therapies. Diabetic foot ulcer management requires multidisciplinary and aggressive approach. PDGF should be recommended for all grade III and IV diabetic foot ulcer at least 8 weeks old. HBO is equally good an option but has limitations and side effects.

  3. Aperçu sur la composition phytochimique et les activités biologiques de Retama monosperma L Bois. (Fabacée

    Directory of Open Access Journals (Sweden)

    BELMOKHTAR Zoubir

    2016-06-01

    Full Text Available An overeview on phytochemical composition and biological activities of Retama monosperma L Bois. (Fabaceae The phytochemical study of Retama monosperma is little discussed in Algeria. This article is a summary of the data and scientific articles published on these phytochemicals properties. In traditional herbal medicine, this species is considered toxic in high doses, but in small doses, it is used as an emetic, purgative, vermifuge, vulnerary, sedative, anthelmintic and antiseptic Alkaloids, flavonoids and terpene compounds are the main phytochemicals known in Retama monosperma. In the last five years, several scientific studies have been published to explain the activity of the extracts of this species, particularly anti - inflammatory, anticancer and antioxidant.

  4. Evaluation of the Antimicrobial Properties of the Essential Oil of Myrtus communis L. against Clinical Strains of Mycobacterium spp.

    Directory of Open Access Journals (Sweden)

    Stefania Zanetti

    2010-01-01

    Full Text Available Mycobacterium tuberculosis is the etiological agent of tuberculosis. The World Health Organization has estimated that 8 million of people develop active TB every year and the situation is complicated by an increase of Mycobacterium tuberculosis strains resistant to drugs used in antitubercular therapy: MDR and XDR-TB. Myrtle leaf extracts, used as an antiseptic in Sardinian traditional medicine, have strong antibacterial activity as several investigations showed. In this study we investigated the antimicrobial properties of the essential oil of Myrtus communis against clinical strains of M. tuberculosis and M. paratuberculosis.

  5. 茶多酚的功效特性及其在军用食品中的开发应用%Functions of Tea Polyphenol and Its Applications in Military Food

    Institute of Scientific and Technical Information of China (English)

    裴晨琛; 程鹏

    2011-01-01

    茶多酚具有抑菌、抗病毒、免疫调节、抗氧化等诸多功能,可在军用食品中开发保健食品、抗氧化剂、防腐剂、食用色素及稳定剂等,应用前景广阔.%Tea polyphenol has many functions such as bacteriostasic activity, anti-viral effect, immunoregulative effect and oxidation resistance. Its applications in military food were introduced, including health food, antioxidant, antiseptic, edible pigment and color stability.

  6. [Prospects of using miramistin for individual prevention of sexual HIV transmission].

    Science.gov (United States)

    Krivorutchenko, Iu L; Andronovskaia, I B

    2013-03-01

    For more than 20 years cationic surfactant Miramistin has been used in Russia and Ukraine as an antiseptic mean for individual prophylaxis of venereal diseases and for the treatment of genitourinary tract and other systems infections. Complete inhibition of HIV-1 activity in vitro by Miramistin in concentrations higher than 0.0075%, has been demonstrated, that allows to consider this detergent as a potent first-generation vaginal microbicide for the prevention of HIV transmission. Higher anti-HIV effect of Miramistin than of nonoxynol-9 and low local toxicity show good prospects of using Miramistin for individual prevention of HIV transmission. PMID:24605621

  7. PHARMACOGNOSTIC, PHYTOCHEMICAL AND PHYSICOCHEMICAL STUDIES OF PIPER NIGRUM LINN. FRUIT (PIPERACEAE

    Directory of Open Access Journals (Sweden)

    P.V. Kadam

    2013-06-01

    Full Text Available Piper species are reported to have great medicinal value in Indian medicine. Fruits of Piper nigrum Linn is commonly known as “Kalimiri” belongs to the family of Piperaceae and widely used as a pungent condiment. Traditionally it is used as antipyretic, diuretic, aphrodisiac, immune-stimulant, antioxidant, hepatoprotective, digestive, rubefacient, counter irritant, antiseptic, antispasmodic agent. Present work is related to standardization of Piper nigrum by using Pharmacognostic (Macroscopy, Cytomorphology, Physical constants and Photochemical study of fruit. All parameters were studied according to the WHO and Pharmacopoeial guidelines to standardize the Piper nigrum.

  8. Bioactive phytoconstituents and plant extracts from genus Heliotropium

    Directory of Open Access Journals (Sweden)

    Naveen Goyal

    2014-01-01

    Full Text Available Heliotropium is a genus of herbs and rarely shrubs of family Boraginaceae. Heliotropium species have been used in folk medicine for the treatment of gout, rheumatism and as antiseptic, febrifuge, cholagogue, anti-inflammatory and healing agents. The alkaloids mainly pyrrolizidine alkaloids are the main constituents of Heliotropium species, which are responsible for several biological activities viz. anti-tumoural, anti-microbial, and anti-viral effects. Phenolic compounds, terpenoids, and quinones have also been reported in this genus. The present review summarizes the various biological studies done on the extracts and bioactive phytoconstituents from the plants of the genus Heliotropium over the past few decades.

  9. Acute and Subchronic Toxic Effects of the Fruits of Physalis peruviana L.

    OpenAIRE

    Basak Ozlem Perk; Sinem Ilgin; Ozlem Atli; Hale Gamze Duymus; Basar Sirmagul

    2013-01-01

    The fruit of Physalis peruviana L. (PPL) has been traditionally used as antispasmodic, diuretic, antiseptic, sedative, and analgesic all over the world. We aimed to perform qualitative content analysis of the fruits of PPL and to clarify the in vitro genotoxicity and in vivo acute and subchronic toxicity of the fruit. Lyophilized fruit juice does not induce genetic damage. In the acute toxicity studies, LD50 value of the fruit was found to be more than 5000 mg kg−1 for both sexes. According t...

  10. Drug: D01730 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D01730 Drug Silver nitrate (JP16/USP); Silver nitrate (TN) Ag. NO3 168.8929 169.873...ing sensory organs 131 Ophthalmic agents 1319 Others D01730 Silver nitrate (JP16/...USP) 2 Agents affecting individual organs 26 Epidermides 266 Emollients 2663 Metallic agents D01730 Silver n...TISEPTICS AND DISINFECTANTS D08A ANTISEPTICS AND DISINFECTANTS D08AL Silver compounds D08AL01 Silver... nitrate D01730 Silver nitrate (JP16/USP) S SENSORY ORGANS S01 OPHTHALMOLOGICALS S01A ANT

  11. Drug: D05840 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D05840 Drug Silver nitrate, toughened (USP) 2Ag. NO3. Cl 310.7669 313.1943 D05840.g...if Caustic ATC code: D08AL01 S01AX02 A mixture of Silver nitrate [DR:D01730] and Silver chloride Anatomical ...08A ANTISEPTICS AND DISINFECTANTS D08AL Silver compounds D08AL01 Silver nitrate D05840 Silver... antiinfectives S01AX02 Silver compounds D05840 Silver nitrate, toughened (USP) CAS: 8007-31-6 PubChem: 4720... nitrate, toughened (USP) S SENSORY ORGANS S01 OPHTHALMOLOGICALS S01A ANTIINFECTIVES S01AX Other

  12. The three modern faces of mercury.

    OpenAIRE

    Clarkson, Thomas W

    2002-01-01

    The three modern "faces" of mercury are our perceptions of risk from the exposure of billions of people to methyl mercury in fish, mercury vapor from amalgam tooth fillings, and ethyl mercury in the form of thimerosal added as an antiseptic to widely used vaccines. In this article I review human exposure to and the toxicology of each of these three species of mercury. Mechanisms of action are discussed where possible. Key gaps in our current knowledge are identified from the points of view bo...

  13. Management of Open Fracture-Dislocation of the Ankle. A Case Report

    Directory of Open Access Journals (Sweden)

    Yaniel Truffin Rodriguez

    2014-12-01

    Full Text Available An open fracture-dislocation of the ankle is a rare injury. The case of a 57-year-old patient without a history of previous diseases who attended the emergency department of the Dr. Gustavo Aldereguía Lima University General Hospital in Cienfuegos after suffering a work-related accident is presented. To repair this serious injury, the surgical cleaning of the joint with antiseptic solutions was performed urgently, the fracture-dislocation was reduced and an osteosynthesis was carried out. Twelve weeks after surgery the patient returned to his previous work.

  14. Myrrh: medical marvel or myth of the Magi?

    Science.gov (United States)

    Nomicos, Effie Y H

    2007-01-01

    Since antiquity, the genus Commiphora is composed of more than 200 species, and has been exploited as a natural drug to treat pain, skin infections, inflammatory conditions, diarrhea, and periodontal diseases. In more recent history, products derived from Commiphora myrrha and various other species of Commiphora are becoming recognized to possess significant antiseptic, anesthetic, and antitumor properties. Traditional practice and evidence-based research have supported that these properties are directly attributable to terpenoids (especially furanosesquiterpenes), the active compounds present in myrrh essential oil. More recently, current studies have focused on applying clinical trial methodologies to validate its use as an antineoplastic, an antiparasitic agent, and as an adjunct in healing wounds.

  15. Perbandingan Pengaruh Larutan dan Gel Klorheksidin Terhadap Kekuatan Geser Perlekatan Breket Logam

    OpenAIRE

    Malayati

    2012-01-01

    The aim of this study was to know the comparison of the influence of chlorhexidine solution and chlorhexidine gel as antiseptic agents on the shear bond strength of metallic brackets. For this purpose, 50 samples of man’s first maxillary premolars which were cleaned and extracted were divided into five equal clusters. Cluster 1 was controlling cluster, cluster 2 was the application of 0.2% of chlorhexidine solution, cluster 3 was the application of 0.2% of chlorhexidine gel, cluster 4 was the...

  16. Comparative Evaluation of CNS Depressant Activity of the Flavonoid Fractions from the Fresh Leaves and Flowers of Ixora coccinea Linn.

    Directory of Open Access Journals (Sweden)

    Sen AK*

    2011-01-01

    Full Text Available Ixora coccinea Linn belonging to the family Rubiaceae is a plant native to India and Sri Lanka. It is also called Jungle Jeranium. It has been traditionally used in eczema, irregular mensturation, diarrhoea, hypertension skin ulcers, as an antiseptic, antipyretic, anti-inflammatory and anticancer agent. Our present work is the study of its CNS depression activity so that it can be used a herbal sedative The flavonoid fractions of leaves and flowers were tested for CNS depressant activity using Actophotometer where both the leaf and flower extracts showed marked CNS depression up to 87.36%and 95.40%, respectively.

  17. ISOLATION AND IDENTIFICATION OF AMYLASE PRODUCING YEASTS IN ‘TELLA’ (ETHIOPIAN LOCAL BEER) AND THEIR AMYLASE CONTRIBUTION FOR ‘TELLA’ PRODUCTION

    OpenAIRE

    Berhanu Andualem; Amare Gessesse

    2013-01-01

    ‘Tella’ is local beer which is used in most part of Ethiopia. It is made from cereals, such as barley, wheat, maize and other crops. Rhamnus prinoides is also used to provide a special aroma and flavor as well as antiseptic agent. The objective of this study is to determine the contribution of amylases from tella yeast isolates and compare with the role of amylase from malt. House hold ‘tella’ samples were collected and plated on starch agar and then amylase positive isolates of yeast were id...

  18. Contact dermatitis presenting as non-healing wound: case report

    OpenAIRE

    Leelavathi M; Le YY; Tohid H; Hasliza AH

    2011-01-01

    Abstract Topical antiseptics are commonly used in the management of minor wounds, burns, and infected skin. These agents are widely used by health professionals and are often self-prescribed by patients as they are easily available over-the-counter. This case illustrates a 73 year old man who presented with a non-healing wound on his right forearm for 4 weeks. The wound started from an insect bite and progressively enlarged with increasing pruritus and burning sensation. Clinically an ill-def...

  19. Copper complexes as 'radiation recovery' agents

    Energy Technology Data Exchange (ETDEWEB)

    Sorenson, J.R.J.

    1989-02-01

    Copper and its compounds have been used for their remedial effects since the beginning of recorded history. As early as 3000 BC the Egyptians used copper as an antiseptic for healing wounds and to sterilise drinking water; and later, ca 1550 BC, the Ebers Papyrus reports the use of copper acetate, copper sulphate and pulverised metallic copper for the treatment of eye infections. These historical uses of copper and its compounds are particularly interesting in the light of modern evidence concerning the use of certain copper complexes for the treatment of radiation sickness and more recently as an adjunct to radiotherapy for cancer patients.

  20. Effect of the activity of the Brazilian polyherbal formulation: Eucalyptus globulus Labill, Peltodon radicans Pohl and Schinus terebinthifolius Radd in inflammatory models

    OpenAIRE

    Karina C.P. Medeiros; Monteiro, Juliana C; Margareth F. F. M. Diniz; Isac A. Medeiros; Bagnólia A. Silva; Márcia R. Piuvezam

    2007-01-01

    The Brazilian polyherbal formulation (BPF) is composed by dyes of Eucalyptus globulus Labill, Peltodon radicans Pohl and Schinus terebinthifolius Raddi in alcohol at 13.3° GL. The formulation is popularly used in Paraíba state, Brazil since 1889 and it is used as an antiseptic and anti-inflammatory medicine. The aim of this study was to evaluate the anti-inflammatory property of the polyherbal formulation. For this purpose it was used the12-O-tetradecanoylphorbol 13-acetate (TPA) and capsaici...

  1. [Staphylococcal scalded skin syndrome in adults].

    Science.gov (United States)

    Mikkelsen, Carsten Sauer; Mikkelsen, Dorthe Bisgaard; Jensen, Thøger Gorm; Vestergaard, Vibeke

    2010-03-01

    We describe a case of adult staphylococcal scalded skin syndrome (SSSS) in a 91-year-old woman. The patient had a Staphylococcus aureus infection in her right ear with redness and yellow secretion. A few days later she developed Nikolsky sign-positive tender enlarged flaccid bullae involving most parts of her body. The bullae ruptured easily and left a erythematous base. The histopathological changes were characteristic for adult SSSS. The patient was well-treated with intravenous dicloxacillin, topical antibiotic and antiseptic treatment. The patient had marked thrombocytosis, but no interest in further examination. PMID:20199754

  2. 纳米材料对常见厌氧致病菌最低浓度的测定%Measurement of nanometer materials on minimal inhibitory concentration of ordinary anaerobic pathogenic bacteria

    Institute of Scientific and Technical Information of China (English)

    熊德鑫; 梁明

    2003-01-01

    @@ INTRODUCTION Nanometer technology is a new cross-linked progressing science. It has quickly be applied into biology or pharmacy field generally for its definite antibacterial effect. We have proved in above paper that the maximum antiseptic concentration is 109 Cfu/ml in 33 ordinary strains isolated from 11 genera. In this experiment, we continue to explore the minimal bacteriostasis of nanometer materials. Through reports of maximal bactericidal concentration and minimal bacteriostasis, we can confirm bacteriostasis effect of nanometer materials and do some preparations on clinical application.

  3. Tetracycline Loaded Collagen/Hydroxyapatite Composite Materials for Biomedical Applications

    Directory of Open Access Journals (Sweden)

    Laura Cristina Rusu

    2015-01-01

    Full Text Available The paper describes the preparation, characterisation, and testing of tetracycline loaded collagen-carboxymethylcellulose/hydroxyapatite ternary composite materials. The synthesis of this drug delivery system consists in two steps: the first step is the mineralization of collagen-carboxymethylcellulose gel while the second step corresponds to the loading of the ternary composite material with tetracycline. The obtained DDS is characterised by physicochemical, morphological, and release behaviour by using FTIR spectroscopy and microscopy, scanning electron microscopy, and UV-VIS spectroscopy. Based on the release study, it can be assumed that tetracycline is released in a prolonged way, assuring at least 6 days of antiseptic properties.

  4. Softlithographic partial integration of surface-active nanoparticles in a PDMS matrix for microfluidic biodevices

    Energy Technology Data Exchange (ETDEWEB)

    Demming, Stefanie; Buettgenbach, Stephanus [Institute for Microtechnology (IMT), Technische Universitaet Braunschweig, Alte Salzdahlumer Strasse 203, 38124 Braunschweig (Germany); Hahn, Anne; Barcikowski, Stephan [Nanotechnology Department, Laser Zentrum Hannover e.V. (LZH), Hollerithallee 8, 30419 Hannover (Germany); Edlich, Astrid; Franco-Lara, Ezequiel; Krull, Rainer [Institute of Biochemical Engineering (IBVT), Technische Universitaet Braunschweig, Gaussstrasse 17, 38106 Braunschweig (Germany)

    2010-04-15

    The mergence of microfluidics and nanocomposite materials and their in situ structuring leads to a higher integration level within microsystems technology. Nanoparticles (Cu and Ag) produced via laser radiation were suspended in Poly(dimethylsiloxane) to permanently modify surface material. A microstructuring process was implemented which allows the incorporation of these nanomaterials globally or partially at defined locations within a microbioreactor (MBR) for the determination of their antiseptic and toxic effects on the growth of biomass. Partially structured PDMS with nanoparticle-PDMS composite. (Abstract Copyright [2010], Wiley Periodicals, Inc.)

  5. Morphology and transport in biodegradable polymer compositions based on poly(3-hydroxybutyrate) and polyamide 54C

    Energy Technology Data Exchange (ETDEWEB)

    Zhul' kina, A. L.; Ivantsova, E. L.; Filatova, A. G.; Kosenko, R. Yu.; Gumargalieva, K. Z.; Iordanskii, A. L., E-mail: iordan@chph.ras.ru [Russian Academy of Sciences, Semenov Institute of Chemical Physics (Russian Federation)

    2009-05-15

    Complex investigation of the equilibrium sorption of water, diffusive transport of antiseptic, and morphology of mixed compositions based on polyoxybutirate and polyamide resin 54C has been performed to develop and analyze new biodegradable polymer compositions for controlled release of medicinal substances. Samples of mixtures were prepared by two methods: pressing under pressure and solvent evaporation from a polymer solution. The samples were compared and their morphology was analyzed by scanning electron microscopy. It is shown that the component ratio in the obtained mixtures affects their morphological, transport, and sorption characteristics.

  6. Application of EDTA in Cosmetics Industry%EDTA在化妆品中的应用

    Institute of Scientific and Technical Information of China (English)

    聂文燕

    2012-01-01

    EATA is a kind of raw material commonly used in cosmetics industry. We summarized the compl- exation effect, the anti-pseudomonas aeruginosa effect, the antiseptic effect of EDTA, and analyzed the mechanism.%EDTA是化妆品行业常用的原料,对EDTA在化妆品中的螯合作用、抗绿脓假单胞菌作用、防腐作用等进行了总结,并对作用机理进行了分析。

  7. Mechanism of action and application of virocids in health care-associated viral infections

    Directory of Open Access Journals (Sweden)

    Babak Shahbaz

    2016-03-01

    Full Text Available Viruses are important causes of acute and chronic diseases in humans. Newer viruses are still being discovered. Apart from frequently causing infections in the general community, many types of viruses are significant nosocomial pathogens that with emerging viruses has become a real issue in medical field. There are specific treatments, vaccine and physical barrier to fight some of these infections. Health care-associated viral infections are an important source of patient’s morbidity and mortality. The method of sterilization or disinfection depends on the intended use of the medical devices (comprising critical, semicritical and noncritical items and failure to perform proper sterilization or disinfection of these items may leads to introduction of viruses, resulting in infection. Disinfection is an essential way in reducing or disruption of transmission of viruses by environmental surfaces, instruments and hands which achieves by chemical disinfectants and antiseptics, respectively. This review discusses about chemical agents with virocids properties (e.g. alcohols, chlorine compounds, formaldehyde, phenolic compounds, glutaraldehyde, ortho-phthaldehyde, hydrogen peroxide, peracetic acid, iodophor, ammonium compounds quaternary, bigunides and so on., mechanisms of action and their applications in health care-associated viral infection control. As well as, we described an overview for hierarchy of viruses in challenge with disinfantans, effective agents on viral inactivation, i.e.targect viruses, viral stability or survival duration time in enviromental surfaces and hands. We explained disinfection of surfaces, challenges in emerging viral pathogens inactivation, viral resistance to chemical disinfectants and antiseptics. Because, there are laboratory studies and clinical evidences for some viruses which viral resistance to biocide or failure to perform proper disinfection can lead to infection outbreaks. Also, we described virucidal

  8. Apparatus and method for continuous production of beverage spirits, industrial alcohol and/or fuel

    Energy Technology Data Exchange (ETDEWEB)

    Ramos de Mattos, A.

    1981-10-13

    A process and apparatus for the continuous production of spirits, industrial EtOH, or fuel, characterized by maintaining the fermenting yeast in the fermenting tank at all times. Periodic treatment with antiseptics and yeast stimulants are performed within the fermentor tank to reactivate the yeast. Optionally, the yeasts can be recovered into an external tank without interrupting the fermentation. The CO/sub 2/ produced during the fermentation is channeled into a tank which contains water to trap any alcohol present. A diagram of the apparatus is given. The advantages of this process and apparatus in terms of efficiency and practicality are discussed.

  9. Orthodontic treatment of dogs on the basis of modern knowledge and own experience

    Directory of Open Access Journals (Sweden)

    Polkowska Izabela

    2014-12-01

    Full Text Available The aim of the study was to demonstrate the orthodontic treatment of malocclusions in dogs, a condition which can lead to cranio-mandibular and functional disorders of the stomatognathic system. The treatment involved the use of maxillofacialorthopaedic appliances, which type depended on the type of disorder and the degree of malocclusion. The applied treatment induced changes in the alveolar bone. Throughout the process of the treatment a great attention was paid to regular brushing off the orthodontic appliance using antiseptics for prophylactic prevention of inflammation of gingival tissue and the palate caused by food getting stuck in the spaces between the teeth.

  10. Silicon nanoparticles produced by femtosecond laser ablation in water as novel contamination-free photosensitizers

    Science.gov (United States)

    Rioux, David; Laferrière, Marie; Douplik, Alexandre; Shah, Duoaud; Lilge, Lothar; Kabashin, Andrei V.; Meunier, Michel M.

    2009-03-01

    We report the synthesis of novel inorganic contamination-free photosensitizers based on colloidal silicon nanoparticles prepared by laser ablation in pure deionized water. We show that such nanoparticles are capable of generating singlet oxygen (1O2) under laser irradiation with a yield estimated at 10% of that of photofrin, which makes them a potential candidate for therapeutics, antiseptics, or disinfectants. We also discuss a model of 1O2 generation and the possibility for optimizing its release. Potential advantages of such novel inorganic photosensitizers include stable and nonphotobleaching 1O2 release, easy removal, and low dark toxicity.

  11. An assessment of skin preparation in upper limb surgery.

    Science.gov (United States)

    Sullivan, P J; Healy, C E; Hirpara, K M; Hussey, A J; Potter, S M; Kelly, J L

    2008-08-01

    Postoperative wound infections remain a major source of upper limb morbidity. The effectiveness of peri-operative human upper limb preparation was determined using a clear fluid antiseptic and an iodine-based solution over 60 and 90 seconds. Less area was missed using iodine over both times and increasing clear solution preparation time from 60 to 90 seconds improved coverage. Surgical experience had little outcome relevance and a 90-second preparation time with either solution was insufficient, with fingers being the sites most commonly missed.

  12. Antimicrobial activity of magnolol and honokiol against periodontopathic microorganisms.

    Science.gov (United States)

    Chang, B; Lee, Y; Ku, Y; Bae, K; Chung, C

    1998-05-01

    Magnolol (1) and honokiol (2), main compounds from the stem bark of Magnolia obovata Thunb., were evaluated for an antimicrobial activity against periodontopathic microorganisms, Porphyromonas gingivalis, Prevotella gingivalis, Actinobacillus actinomycetemcomitans, Capnocytophaga gingivalis, and Veillonella disper, and a cytotoxicity against human gingival fibroblasts and epithelial cells. Our results indicate that magnolol and honokiol, although less potent than chlorhexidine, show a significant antimicrobial activity against these microorganisms, and a relatively low cytotoxic effect on human gingival cells. Thus, it is suggested that magnolol and honokiol may have a potential therapeutic use as a safe oral antiseptic for the prevention and the treatment of periodontal disease. PMID:9619121

  13. -Sitosterol-3-O--D-xylopyranoside from the flowers of Tridax procumbens Linn.

    Indian Academy of Sciences (India)

    V K Saxena; Sosanna Albert

    2005-05-01

    Tridax procumbens Linn belongs to the natural order Compositae and is locally known as `Ghamra’. It has been found to possess significant medicinal properties. Its leaves are used in bronchial catarrh, dysentery, diarrhoea and to prevent falling of hair. Its flowers and leaves possess antiseptic, insecticidal and parasiticidal properties, and are also used to check haemorrhage from cuts, bruises and wounds. The present work deals with the isolation and identification of steroidal saponin, characterized as -sitosterol 3-O--D-xylopyranoside, which has been isolated from the flowers of Tridax procumbens Linn.

  14. Botanical medicines for the urinary tract.

    Science.gov (United States)

    Yarnell, Eric

    2002-11-01

    Four important categories of urologic herbs, their history, and modern scientific investigations regarding them are reviewed. Botanical diuretics are discussed with a focus on Solidago spp (goldenrod) herb, Levisticum officinale (lovage) root, Petroselinum crispus (parsley) fruit, and Urtica dioica (stinging nettle) herb. Urinary antiseptic and anti-adhesion herbs, particularly Arctostaphylos uva-ursi (uva-uri) leaf, Juniperus spp (juniper) leaf, and Vaccinium macrocarpon (cranberry) fruit are reviewed. The antinephrotoxic botanicals Rheum palmatum (Chinese rhubarb) root and Lespedeza capitata (round-head lespedeza) herb are surveyed, followed by herbs for symptoms of benign prostatic hyperplasia, most notably Serenoa repens (saw palmetto) fruit, Urtica dioica root, and Prunus africana (pygeum) bark.

  15. Aloe Vera in Dentistry

    OpenAIRE

    Sujatha, G; Kumar, G. Senthil; Muruganandan, J; Prasad, T Srinivasa

    2014-01-01

    Aloe vera is a medicinal plant which has been used for thousands of years. The health benefits of aloe vera is well known and the dental uses of this plant is multiple. Interest is gathering among researchers regarding the use of this plant. Studies have proved the antiseptic, anti inflammatory, antiviral and antifungal properties of aloe vera and the use of this plant is proved beneficial. This plant is proved to be non allergic and very good in building up the immune system. Aloe vera is ga...

  16. A novel in vitro wound biofilm model used to evaluate low-frequency ultrasonic-assisted wound debridement

    DEFF Research Database (Denmark)

    Crone, S.; Garde, Christian; Bjarnsholt, T.;

    2015-01-01

    device (UA W) in the presence of saline irrigation and treatment with a polyhexamethylene biguanide (PHMB)-containing antiseptic. Confocal microscopy was used to evaluate the effect of treatments on biofilm disruption and cell viability counting measured the antibacterial effects. Results: Confocal...... microscopy showed that application of 10 seconds of moderate-intensity UA W could effectively disrupt semi-solid biofilms grown on both media settings. This treatment only had a small effect on the cell viability. A 24-hour treatment with PHMB was able to reduce the number of bacteria but not eradicate...

  17. Application of topical drugs in burn wound%烧伤创面用药

    Institute of Scientific and Technical Information of China (English)

    张国安

    2008-01-01

    For bum patients, topical treatment is as important as systemic treatment. Reasonable and timely wound treatment will influence the homeostatic equilibrium, and the progression, the prognosis, and the outcome of the disease. The therapeutic principle should be varied for wounds with different depth of injury. But avoiding or at least alleviating infection, and accelerating healing period, were the common principles. In common, the medication for local wound treatment includes: topical antiseptic, surgical dressing products, artificial skins, and so on.Ideal topical antiseptic should have the following zcharacteristics: the antimierobial spectrum is broad, including Pseudomonas aeruginosa and MRSA; be able to penetrate necrotic tissue; does not induce drug resistatance easily; no local irritating effect and not painful; no side effect to body; can be applied easily; low cost. The functions of surgical dressing in clude: protect the wounds, keep the microcirculation open, and accelerate wound healing. Artificial skin has been used as the autoskin carrier in skin transplantation operation for large burn area to protect the autoskin grafts, accelerate wound healing, and cover the wounds temporarily. Bums therapy has developed for 50 years in China, the study of local treatment for burn wounds has also experienced a tortuous path of trial and error. This review might contribute some ideas future research.

  18. Prospects of zinc nano oxide application in the treatment of purulent wounds

    Directory of Open Access Journals (Sweden)

    Popadyuk O.Ya.

    2015-03-01

    Full Text Available Surgical infection is a difficult problem in modern medical practice. Prevalence of surgical infection, new strains of microorganisms and their resistance to antibiotics, poor outcomes of wound treatment, lack of sensitivity of microorganisms to commonly used antiseptics necessitate the search for the new methods and means of treatment in wound surgery. This article provides an overview of domestic and foreign literature on the possibility of solving the problem of effective local treatment of purulent wounds through the study and implementation of nanotechnology in modern medicine. Researchers around the world are beginning to use nanoparticles and developments of nanotechnology in various fields of science and medicine to synthesize new drugs and vaccines, including zinc nano oxide usage as a highly effective local antiseptic that is non-toxic to the cells of the human body at very low, concentrations but with sufficient antibacterial action. Small sizes and large surface area relatively to volume increases efficiency of interaction of nanoparticles with germs and makes it probable a wide range of antimicrobial activity. Nanoparticles on metals base due to their biological and physical-chemical properties are perspective antibacterial agents and may be used to solve many problems in nanomedicine and surgery in particular. Study and application of zinc nano oxide in preparations of multidirectional local action will ensure high effective defense against pathogenic microorganisms in the wound.

  19. The in Vitro Antimicrobial Efficacy of Propolis against Four Oral Pathogens: A Review

    Directory of Open Access Journals (Sweden)

    Nadine Michèle Waldner-Tomic

    2014-07-01

    Full Text Available This study screened the available evidence for the in vitro antimicrobial efficacy of propolis, a natural herbal resin bee product, against a selection of three bacterial species of relevance to oral diseases. For this purpose, papers dealing with laboratory studies assessing minimum inhibitory concentration (MIC, minimum bactericidal concentrations (MBC or the agar diffusion method to analyze the antimicrobial properties of propolis on three oral pathogens (S. mutans, P. gingivalis, F. nucleatum and a yeast (C. albicans are reviewed. Overall, a positive antimicrobial effect could be shown. However, when compared to the commonly used control substances (e.g., specific antibiotics, antiseptics and antifungals, propolis appeared less effective, depending on the bacterial strain, and required higher concentrations than the control substances, in order to show a measurable effect. Nevertheless, propolis as a natural herbal resin bee product can be considered as a natural antiseptic agent within the range of other herbal products, like sanguinarine. Therefore, it may be a valuable compound of non-synthetic, natural origin for patients seeking complementary agents and alternatives for “hard” chemicals.

  20. Chemical Characterization and in Vitro Antibacterial Activity of Myrcianthes hallii (O. Berg McVaugh (Myrtaceae, a Traditional Plant Growing in Ecuador

    Directory of Open Access Journals (Sweden)

    Patricia Chavez Carvajal

    2016-06-01

    Full Text Available Myrcianthes hallii (O. Berg McVaugh (Myrtaceae is a plant native to Ecuador, traditionally used for its antiseptic properties. The composition of the hydro-methanolic extract of this plant was determined by submitting it to ultra-high performance liquid chromatography (UHPLC hyphenated to heated-electrospray ionization mass spectrometry and UV detection. The presence of antimicrobial components prompted us to test the extract against methicillin-resistant and methicillin-susceptible Staphylococcus aureus, multidrug-resistant and susceptible Escherichia coli, Pseudomonas aeruginosa, Enterococcus spp. and Streptococcus pyogenes strains. The chromatographic analysis led to the identification of 38 compounds, including polyphenols and organic acids, and represents the first chemical characterization of this plant. The extract showed modest antibacterial activity against all tested bacteria, with the exception of E. coli which was found to be less sensitive. Whilst methicillin-resistant strains usually display resistance to several drugs, no relevant differences were observed between methicillin-susceptible and resistant strains. Considering its long-standing use in folk medicine, which suggests the relative safety of the plant, and the presence of many known antibacterial polyphenolic compounds responsible for its antibacterial activity, the results show that M. hallii extract could be used as a potential new antiseptic agent. Moreover, new anti-infective biomaterials and nanomaterials could be designed through the incorporation of M. hallii polyphenols. This prospective biomedical application is also discussed.

  1. The nitric acid burn trauma of the skin.

    Science.gov (United States)

    Kolios, L; Striepling, E; Kolios, G; Rudolf, K-D; Dresing, K; Dörges, J; Stürmer, K M; Stürmer, E K

    2010-04-01

    Nitric acid burn traumata often occur in the chemical industry. A few publications addressing this topic can be found in the medical database, and there are no reports about these traumata in children. A total of 24 patients, average 16.6 years of age, suffering from nitric acid traumata were treated. Wound with I degrees burns received open therapy with panthenol-containing creams. Wound of II degrees and higher were initially treated by irrigation with sterile isotonic saline solution and then by covering with silver-sulphadiazine dressing. Treatment was changed on the second day to fluid-absorbent foam bandages for superficial wounds (up to IIa degrees depth) and occlusive, antiseptic moist bandages in combination with enzymatic substances for IIb degrees -III degrees burns. After the delayed demarcation, necrectomy and mesh-graft transplantation were performed. All wounds healed adequately. Chemical burn traumata with nitric acid lead to specific yellow- to brown-stained wounds with slower accumulation of eschar and slower demarcation compared with thermal burns. Remaining wound eschar induced no systemic inflammation reaction. After demarcation, skin transplantation can be performed on the wounds, as is commonly done. The distinguishing feature of nitric-acid-induced chemical burns is the difficulty in differentiation and classification of burn depth. An immediate lavage should be followed by silver sulphadiazine treatment. Thereafter, fluid-absorbent foam bandages or occlusive, antiseptic moist bandages should be used according to the burn depth. Slow demarcation caused a delay in performing surgical treatments. PMID:19875347

  2. Disinfection of the Access Orifice in NOTES: Evaluation of the Evidence Base

    Directory of Open Access Journals (Sweden)

    Mikael H. Sodergren

    2011-01-01

    Full Text Available Introduction. Appropriate prevention of infection is a key area of research in natural orifice translumenal endoscopic surgery (NOTES, as identified by the Natural Orifice Surgery Consortium for Assessment and Research (NOSCAR. Methods. A review of the literature was conducted evaluating the evidence base for access orifice preparation/treatment in NOTES procedures in the context of infectious complications. Recommendations based on the Oxford Centre for Evidence-Based Medicine guidelines were made. Results. The most robust evidence includes several experimental randomised controlled trials assessing infectious complications in the transgastric approach to NOTES. Transvaginal procedures are long established for accessing the peritoneal cavity following disinfection with antiseptic. Only experimental case series for transcolonic and transvesical approaches are described. Conclusion. Grade C recommendation requiring no preoperative preparation can be made for the transgastric approach. Antiseptic irrigation is recommended for transvaginal (grade C NOTES access, as is current practice. Further human trials need to be conducted to corroborate the current evidence base for transgastric closure. It is important that future trials are conducted in a methodologically robust fashion, with emphasis on clinical outcomes and standardisation of enterotomy closure and postoperative therapy.

  3. Preparation and physicochemical properties of surfactant-free emulsions using electrolytic-reduction ion water containing lithium magnesium sodium silicate.

    Science.gov (United States)

    Okajima, Masahiro; Wada, Yuko; Hosoya, Takashi; Hino, Fumio; Kitahara, Yoshiyasu; Shimokawa, Ken-ichi; Ishii, Fumiyoshi

    2013-04-01

    Surfactant-free emulsions by adding jojoba oil, squalane, olive oil, or glyceryl trioctanoate (medium chain fatty acid triglycerides, MCT) to electrolytic-reduction ion water containing lithium magnesium sodium silicate (GE-100) were prepared, and their physiochemical properties (thixotropy, zeta potential, and mean particle diameter) were evaluated. At an oil concentration of 10%, the zeta potential was ‒22.3 ‒ ‒26.8 mV, showing no marked differences among the emulsions of various types of oil, but the mean particle diameters in the olive oil emulsion (327 nm) and MCT emulsion (295 nm) were smaller than those in the other oil emulsions (452-471 nm). In addition, measurement of the hysteresis loop area of each type of emulsion revealed extremely high thixotropy of the emulsion containing MCT at a low concentration and the olive emulsion. Based on these results, since surfactants and antiseptic agents markedly damage sensitive skin tissue such as that with atopic dermatitis, surfactant- and antiseptic-free emulsions are expected to be new bases for drugs for external use.

  4. Delayed norovirus epidemic in the 2009-2010 season in Japan: potential relationship with intensive hand sanitizer use for pandemic influenza.

    Science.gov (United States)

    Inaida, S; Shobugawa, Y; Matsuno, S; Saito, R; Suzuki, H

    2016-09-01

    Norovirus (NoV) epidemics normally peak in December in Japan; however, the peak in the 2009-2010 season was delayed until the fourth week of January 2010. We suspected intensive hand hygiene that was conducted for a previous pandemic influenza in 2009 as the cause of this delay. We analysed the NoV epidemic trend, based on national surveillance data, and its associations with monthly output data for hand hygiene products, including alcohol-based skin antiseptics and hand soap. The delayed peak in the NoV incidence in the 2009-2010 season had the lowest number of recorded cases of the five seasons studied (2006-2007 to 2010-2011). GII.4 was the most commonly occurring genotype. The monthly relative risk of NoV and monthly output of both alcohol-based skin antiseptics and hand soap were significantly and negatively correlated. Our findings suggest an association between hand hygiene using these products and prevention of NoV transmission. PMID:27301793

  5. Skin care: an essential component of hand hygiene and infection control.

    Science.gov (United States)

    Bissett, Linda

    Skin care is an important component of hand hygiene and also infection prevention education programmes relating to hand hygiene (Bissett, 2007a,b). Hand hygiene is the term commonly used to describe hand washing using plain soap or antiseptic soaps and hand rubbing using waterless antiseptic products or alcohol-based products. The importance of effective hand hygiene is well documented (Larson, 1997; Boyce et al, 2002; Horton and Parker, 2002) and can be achieved by following the six-step technique used for hand washing as illustrated by the Royal College of Nursing (2000). During hand washing, hand soaps not only remove soils, but also the natural oils that protect the skin. This can vary depending on the frequency of hand washing, the temperature of the water and the ability of the soap to be rinsed from the surface of the hands (Starobin, 2007). This article aims to examine the evidence available to enable healthcare staff to make an informed decision on the importance of following a skin care regime to reduce the risk of bacterial loading on the hands caused by damaged skin. This would consequently lead to an improvement in hand hygiene efficacy.

  6. Assessment of oral malodor and tonsillar microbiota after gargling with benzethonium chloride.

    Science.gov (United States)

    Iwamura, Yuki; Hayashi, Jun-Ichiro; Sato, Takashi; Sato, Sota; Murakami, Taeko; Fujimura, Takeki; Sasaki, Yasuyuki; Okada, Kosuke; Takahashi, Eri; Kikuchi, Takeshi; Aino, Makoto; Noguchi, Toshihide; Shimazaki, Yoshihiro; Mitani, Akio; Fukuda, Mitsuo

    2016-01-01

    The oropharyngeal area can be a source of halitosis. However, the relationship between healthy tonsillar microbiota and halitosis is poorly understood. We conducted a pilot clinical study to clarify the effect of gargling with an antiseptic agent on tonsillar microbiota in patients with halitosis. Twenty-nine halitosis patients who did not have otolaryngologic disease or periodontitis were assigned randomly to one of three groups: benzethonium chloride (BZC) gargle; placebo gargle; no gargle. Concentrations of volatile sulfur compounds (VSCs) in mouth air, the organoleptic score (ORS) and tongue-coating score (TCS) were measured before and after testing. Tonsillar microbiota were assessed by detection of periodontal pathogens, and profiling with terminal-restriction fragment length polymorphism (T-RFLP) analysis and sequencing of 16SrRNA clone libraries for taxonomic assignment. Gargling with BZC reduced the concentrations of methyl mercaptan and hydrogen sulfide and the ORS, but did not affect the TCS or prevalence of periodontal pathogens. T-RFLP analyses and 16SrRNA clone sequencing showed a tendency for some candidate species to decrease in the test group. Although gargling of the oropharyngeal area with an antiseptic agent can reduce oral malodor, it appears that tonsillar microbiota are not influenced greatly. (J Oral Sci 58, 83-91, 2016). PMID:27021544

  7. Enhanced chlorhexidine skin penetration with eucalyptus oil

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    Worthington Tony

    2010-09-01

    Full Text Available Abstract Background Chlorhexidine digluconate (CHG is a widely used skin antiseptic, however it poorly penetrates the skin, limiting its efficacy against microorganisms residing beneath the surface layers of skin. The aim of the current study was to improve the delivery of chlorhexidine digluconate (CHG when used as a skin antiseptic. Method Chlorhexidine was applied to the surface of donor skin and its penetration and retention under different conditions was evaluated. Skin penetration studies were performed on full-thickness donor human skin using a Franz diffusion cell system. Skin was exposed to 2% (w/v CHG in various concentrations of eucalyptus oil (EO and 70% (v/v isopropyl alcohol (IPA. The concentration of CHG (μg/mg of skin was determined to a skin depth of 1500 μm by high performance liquid chromatography (HPLC. Results The 2% (w/v CHG penetration into the lower layers of skin was significantly enhanced in the presence of EO. Ten percent (v/v EO in combination with 2% (w/v CHG in 70% (v/v IPA significantly increased the amount of CHG which penetrated into the skin within 2 min. Conclusion The delivery of CHG into the epidermis and dermis can be enhanced by combination with EO, which in turn may improve biocide contact with additional microorganisms present in the skin, thereby enhancing antisepsis.

  8. Improving stability of a novel dextran-degrading enzyme from marine Arthrobacter oxydans KQ11.

    Science.gov (United States)

    Wang, Delong; Lu, Mingsheng; Wang, Xiaobei; Jiao, Yuliang; Fang, Yaowei; Liu, Zhaopu; Wang, Shujun

    2014-03-15

    Dextranases can hydrolyze dextran, so they are used in the sugar industry to mitigate the milling problems associated with dextran contamination. Few studies have been carried out on the storage stability of dextranase, let alone the dextranase of Arthrobacter oxydans KQ11 isolated from sea mud samples. This study improved the storage stability of dextranase from marine A. oxydans KQ11 by adding enzymatic protective reagents (stabilizer and antiseptic). Initially, the conditions (55 °C and 30 min) for maintaining 50% dextranase activity were obtained. Then, the best stabilizers of dextranase were obtained, namely, glycerol (16%), sodium acetate (18%) and sodium citrate (20%). Results showed that p-hydroxybenzoic acid compound sodium acetate (0.05%), D-sodium isoascorbiate (0.03%), and potassium sorbate (0.05%) were the best antiseptics. Subsequent validation experiment showed that dextranase with enzymatic protective reagents maintained 70.8% and 28.96% activities at the 13th week at 25 and 37 °C, respectively. PMID:24528732

  9. Application of the low-level laser therapy for the treatment of vaginitis

    Science.gov (United States)

    Passeniouk, A. N.; Mikhailov, V. A.

    2000-06-01

    Vaginitis is the most common female infectious disease. Females suffering from this disorder are annually increasing in number. There are a lot of modalities of treatment of vaginitis, but because of drug allergy and microbe's stability to drug the treatment of vaginitis is difficult. Our study compares the efficacy of laser-therapy with drug therapy in the treatment of non-specific vaginitis and vaginal candidiasis. Thirty women reci4eed the LLLT by local action with antiseptic liquid daily during ten days, 20 women received metronidazole and fluconozole and vaginal application of metronidazole. The results suggest that local laser-therapy is able to remove sights of vaginitis more efficiently and faster than drug therapy. Repair of normal vaginal microflora, which is the best indicator of recovery, was significantly at a faster rate in laser-therapy group. There were no report of adverse reaction with vaginal laser- therapy, whereas there were women on drug therapy who reported side effects. In conclusion, vaginal aser-therapy with antiseptic liquid is a suitable, effective, safe and chip alternative to drug therapy in the treatment of vaginitis.

  10. Guidelines for hand hygiene in hospital

    Directory of Open Access Journals (Sweden)

    Christina Sotnikova

    2013-10-01

    Full Text Available The appropriate compliance of rules regarding hand hygiene, have been and continue to be, one of the basic points of all prevention programs of nosocomial infections. Aim: The aim of the present study was the presentation of guidelines for hand hygiene by nurses. Method and material: Study of international and Greek literature from electronic databases Medline, PubMed and scientific journals, KEELPNO mainly from the last three years. The keywords used were: nosocomial infections, hand hygiene, antiseptics and guidelines. Results: There are numerous studies that demonstrate the central role of hand hygiene of employees in transmission of microorganisms in the hospital environment. Hand hygiene is a process, which should not be omitted or replaced by the use of antiseptics. The professionals position in the hospital, the workplace and staff shortages, in conjunction with the large number of hospitalized patients seem to be the main factors that negatively affect the compliance with hand hygiene. The staff working in the hospital, especially with high-risk patients (ICU, surgery should not wear artificial nails, nail polish and jewelry - rings and should not have inflammation or infection on their hands. Conclusions: Hand hygiene should be applied before and after contact with each patient. Nurses have to comply with these guidelines, in order for the effective prevention and control of nosocomial infections.

  11. Antimicrobial functionalization of bacterial nanocellulose by loading with polihexanide and povidone-iodine.

    Science.gov (United States)

    Wiegand, Cornelia; Moritz, Sebastian; Hessler, Nadine; Kralisch, Dana; Wesarg, Falko; Müller, Frank A; Fischer, Dagmar; Hipler, Uta-Christina

    2015-10-01

    Bacterial nanocellulose (BNC) is chemically identical with plant cellulose but free of byproducts like lignin, pectin, and hemicelluloses, featuring a unique reticulate network of fine fibers. BNC sheets are mostly obtained by static cultivation. Now, a Horizontal Lift Reactor may provide a cost efficient method for mass production. This is of particular interest as BNC features several properties of an ideal wound dressing although it exhibits no bactericidal activity. Therefore, BNC was functionalized with the antiseptics povidone-iodine (PI) and polihexanide (PHMB). Drug loading and release, mechanical characteristics, biocompatibility, and antimicrobial efficacy were investigated. Antiseptics release was based on diffusion and swelling according to Ritger-Peppas equation. PI-loaded BNC demonstrated a delayed release compared to PHMB due to a high molar drug mass and structural changes induced by PI insertion into BNC that also increased the compressive strength of BNC samples. Biological assays demonstrated high biocompatibility of PI-loaded BNC in human keratinocytes but a distinctly lower antimicrobial activity against Staphylococcus aureus compared to PHMB-loaded BNC. Overall, BNC loaded with PHMB demonstrated a better therapeutic window. Moreover, compressive and tensile strength were not changed by incorporation of PHMB into BNC, and solidity during loading and release could be confirmed. PMID:26411441

  12. Contact dermatitis presenting as non-healing wound: case report

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    Leelavathi M

    2011-05-01

    Full Text Available Abstract Topical antiseptics are commonly used in the management of minor wounds, burns, and infected skin. These agents are widely used by health professionals and are often self-prescribed by patients as they are easily available over-the-counter. This case illustrates a 73 year old man who presented with a non-healing wound on his right forearm for 4 weeks. The wound started from an insect bite and progressively enlarged with increasing pruritus and burning sensation. Clinically an ill-defined ulcer with surrounding erythema and erosion was noted. There was a yellow crust overlying the center of the ulcer and the periphery was scaly. Further inquiry revealed history of self treatment with a yellow solution to clean his wound for 3 weeks. Patient was provisionally diagnosed to have allergic contact dermatitis secondary to acriflavine. Topical acriflavine was stopped and the ulcer resolved after treatment with non-occlusive saline dressing. Skin patch test which is the gold standard for detection and confirmation of contact dermatitis showed a positive reaction (2+ to acriflavine. Acriflavine is widely used as a topical antiseptic agent in this part of the world. Hence, primary care physicians managing a large variety of poorly healing wounds should consider the possibility of contact allergy in recalcitrant cases, not responding to conventional treatment. Patient education is an important aspect of management as this would help curb the incidence of future contact allergies.

  13. Medical Plasma in Dentistry: A Future Therapy for Peri-implantitis

    Science.gov (United States)

    Koban, Ina; Jablonowski, Lukasz; Kramer, Axel; Weltmann, Klaus-Dieter; Kocher, Thomas

    Biofilm formation plays a major role in the pathogenesis of many oral diseases especially in peri-implantits. To evaluate the anti-biofilm effect of different plasma devices and processes we used different dental biofilm models: Candida albicans, Streptococcus mutans, Streptococcus sanguinis, aerobe multispecies human saliva and anaerobe plaque biofilms. After 10 min treatment we reduced the biofilms by 5 log10 steps using dielectric barrier discharge (DBD) plasma. Chlorhexidine is the gold standard antiseptic which achieved in the same time only a 1.5 log10 reduction. All plasma devices (DBD or plasma jets) damaged the membrane of the microorganisms but only etching plasma sources can remove the biofilm as shown in CLSM micrographs. It is possible to improve the plasma process using antiseptics like octenidine. This combination significantly reduced CFU values after 1 min plasma treatment compared to the plasma control. Beside the anti-biofilm effect an additional effect of plasma is the contact angle reduction of different titanium implant surfaces from 90° to super-hydrophilic (<5°). This can improve the implant healing process. Thus in the future, plasma could be an interesting treatment option in dentistry, especially in treatment of peri-implantits.

  14. Rapid anti-pathogen response in ant societies relies on high genetic diversity.

    Science.gov (United States)

    Ugelvig, Line V; Kronauer, Daniel J C; Schrempf, Alexandra; Heinze, Jürgen; Cremer, Sylvia

    2010-09-22

    Social organisms are constantly exposed to infectious agents via physical contact with conspecifics. While previous work has shown that disease susceptibility at the individual and group level is influenced by genetic diversity within and between group members, it remains poorly understood how group-level resistance to pathogens relates directly to individual physiology, defence behaviour and social interactions. We investigated the effects of high versus low genetic diversity on both the individual and collective disease defences in the ant Cardiocondyla obscurior. We compared the antiseptic behaviours (grooming and hygienic behaviour) of workers from genetically homogeneous and diverse colonies after exposure of their brood to the entomopathogenic fungus Metarhizium anisopliae. While workers from diverse colonies performed intensive allogrooming and quickly removed larvae covered with live fungal spores from the nest, workers from homogeneous colonies only removed sick larvae late after infection. This difference was not caused by a reduced repertoire of antiseptic behaviours or a generally decreased brood care activity in ants from homogeneous colonies. Our data instead suggest that reduced genetic diversity compromises the ability of Cardiocondyla colonies to quickly detect or react to the presence of pathogenic fungal spores before an infection is established, thereby affecting the dynamics of social immunity in the colony. PMID:20444720

  15. ALA-based fluorescent diagnosis of malignant oral lesions in the presence of bacterial porphyrin formation

    Science.gov (United States)

    Schleier, P.; Berndt, A.; Zinner, K.; Zenk, W.; Dietel, W.; Pfister, W.

    2006-02-01

    The aminolevulinic acid (5-ALA) -based fluorescence diagnosis has been found to be promising for an early detection and demarcation of superficial oral squamous cell carcinomas (OSCC). This method has previously demonstrated high sensitivity, however this clinical trial showed a specificity of approximately 62 %. This specificity was mainly restricted by tumor detection in the oral cavity in the presence of bacteria. After topical ALA application in the mouth of patients with previously diagnosed OSSC, red fluorescent areas were observed which did not correlate to confirm histological findings. Swabs and plaque samples were taken from 44 patients and cultivated microbiologically. Fluorescence was investigated (OMA-system) from 32 different bacteria strains found naturally in the oral cavity. After ALA incubation, 30 of 32 strains were found to synthesize fluorescent porphyrins, mainly Protoporphyrin IX. Also multiple fluorescent spectra were obtained having peak wavelengths of 636 nm and around 618 nm - 620 nm indicating synthesis of different porphyrins, such as the lipophylic Protoporphyrin IX (PpIX) and hydrophylic porphyrins (water soluble porphyrins, wsp). Of the 32 fluorescent bacterial strains, 18 produced wsp, often in combination with PpIX, and 5 produced solely wsp. These results clarify that ALA-based fluorescence diagnosis without consideration or suppression of bacteria fluorescence may lead to false-positive findings. It is necessary to suppress bacteria fluorescence with suitable antiseptics before starting the procedure. In this study, when specific antiseptic pre-treatment was performed bacterial associated fluorescence was significantly reduced.

  16. 二氧化氯在循环水杀菌灭藻中出现的问题及解决措施%PROBLEMS AND .RESOLUTIONS IN STERILIZATION OF RECYCLED WATER WITH CHLORINE DIOXIDE

    Institute of Scientific and Technical Information of China (English)

    熊梦辉; 覃华锋

    2012-01-01

    The necessity in using chlorine dioxide to replace liquid chlorine as antiseptic for recycled water system is analyzed based upon both the mechanism and safety,the application of chlorine dioxide to ammonia recycled water system is introduced and the problem therefrom analyzed and resolutions put forward for improvement, the obvious effect shows that the application of chlorine dioxide to replace liquid chlorine as antiseptic in recycled water system is successful.%从杀菌机理和安全性分析循环水系统用二氧化氯替代液氯作为杀菌灭藻剂的必要性,介绍二氧化氯在合成氨循环水系统的应用情况,对出现的问题进行分析并提出相应措施进行改进,取得了明显的效果,应用二氧化氯替代液氯在循环水系统中作为杀菌剂是成功的。

  17. Study on combination of not contain bivalent salt KATHON and DMDMH%不含二价盐卡松与 DMDMH 的复配增效研究

    Institute of Scientific and Technical Information of China (English)

    徐亚霞; 李程碑; 杨俊伟

    2014-01-01

    Inhibition trend of nonsalt KATHON and DMDMH on bacteria and epiphyte was evaluated for by means of inhibition zone. Best combined antiseptic system as ppm ratio is 0. 60: 200(mg / L)was de-termined by means of synergy coefficient experiment. Challenging experiment was used in order to validate the bacteriostasis of the combined system,and it was possible to be used as antiseptic daily cosmetic of in-dustry.%应用抑菌圈法测试无盐卡松与 DMDMH 对细菌和真菌的抑制生长趋势,应用增效系数法实验确定了它们的最佳复配体系0.60:200(mg/ L),应用挑战实验法验证了该体系优良的抑制细菌、真菌生长效果,使其有望作为日化等行业防腐剂使用。

  18. Mouthwashes with hydrogen peroxide are carcinogenic, but are freely indicated on the internet: warn your patients!

    Directory of Open Access Journals (Sweden)

    Alberto Consolaro

    2013-12-01

    Full Text Available It all began in Ancient Egypt where people used to bleach their teeth with antiseptic mouthwashes made of urea from human urine. Teeth harmony is promoted by expression of feelings, communication, a real window of the brain and its content! Tooth bleaching products are medicines, not cosmetics! Mouth washing with hydrogen peroxide is an illogical and dangerous procedure! Hydrogen peroxide must be used in one's mouth only when employed by a dentist who has been properly instructed to protect the mucosa, preventing it from receiving these products. How and for how long these products are going to be used require caution in order to avoid or decrease any adverse effects on the tissues. Many websites instruct people on how to purchase and prepare hydrogen peroxide so that it is used as an antiseptic mouthwash and tooth bleaching agent. Some websites even refer to dentists as "exploiters", accusing them of not instructing patients properly. In this article, we aim at providing evidence and information upon which dentists and assistants may base their thinking as well as their opinion and procedures regarding "the indiscriminate and free use of hydrogen peroxide in the mouth, on teeth and oral mucosa". Those websites, blogs and social network profiles trespass the limits of public trust and should be immediately sued by the government for committing a crime against public health.

  19. THE STUDY THE EFFICACY AND SAFETY OF ANTIMICROBIAL AGENTS

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    V. V. Bagaeva

    2015-01-01

    Full Text Available Abstract:Effective treatment of patients with infectious and inflammatory diseases of the skin and mucous membranes often involves the use of antimicrobial agents.The purpose of the study was an in vitro estimation of cytotoxicity and the efficiency of national resources for local use: gel with bacteriophages («Otofag», «Fagogin», «Fagoderm», «Fagodent» and antiseptic — «Сhlorhexidine» and «Miramistin».Materials and Methods. To study the effectiveness of antimicrobial agents they used to provide crop strains of Staphylococcus aureus and Streptococcus pyogenes as one of the most common representatives of pathogens. The study of cell viability and cytotoxicity antimicrobials performed on cell lines KB — epidermoid carcinoma of the oral cavity of a human. For this purpose we use mikrotetrazoly test, which is widely used in the assessment of the effects on the cells of toxins, pharmaceuticals, adverse environmental factors, allowing to evaluate the toxicity of investigational drugs in vitro.The results showed that the efficacy against pathogens Staphylococcus aureus and Streptococcus pyogenes, has even a 10‑fold dilution of «Сhlorhexidine» 0.05% and gels with bacteriophages. Antiseptic «Miramistin» is effective only on the initial concentration. The study of cytotoxicity showed that the processing of epidermoid carcinoma cells with «Chlorhexidine» and «Мiramistin» invokes the irreversible reactions, while the composition processing of gels based on bacteriophages not further affect cell viability.Conclusions The results of the experiment confirmed the significant toxicity of tools such as «Сhlorhexidine» and «Miramistin» in proposed concentrations in the pharmacy network. Despite the high efficiency of these vehicles with regard to the studied pathogens, their long-term use in treatment of inflammatory diseases of the skin and mucous membranes can cause a slowing of repair processes. Gel means with bacteriophages

  20. Fresh preservation of roast chicken%烧鸡的防腐与保鲜

    Institute of Scientific and Technical Information of China (English)

    谷小慧; 农绍庄; 崔瑾; 张琪

    2012-01-01

    Study of synthetic preserving techniques is carried out in this thesis,which bases on roast chicken.On the best culture condition,apply different antiseptic agents to chicken bacterium so as to seek out optimum antiseptic agents through different bacteriostatic effects.Finally,keep the roast chicken combining with vacuum packaging and microwave sterilization.The results indicated that e optimum antiseptic agent are 0.05% Nisin,0.05% lysozyme,1% sodium diacedete.Soak the roast chicken in this multiple preservative solution for 60 seconds,and then adopt vacuum packaging and microwave sterilization together.The shelf-life of roast chicken which was processed could be extended at least 11 days at 36 ℃,and all of the indicators are permitted in the national standards range,prolong the valid date.%实验以烧鸡鸡肉为原材料,对烧鸡的防腐保鲜进行初步研究。根据培养条件培养鸡肉细菌来做抑菌试验,通过对不同防腐保鲜液的抑菌实验,筛选出最佳保鲜液;再结合真空包装和微波杀菌的保鲜方法,进行贮藏。结果表明:最佳复合保鲜剂配方为Nisin浓度0.05,溶菌酶浓度0.05,双乙酸钠浓度1的保鲜液。将烧鸡鸡肉浸泡于此保鲜液中60s,再进行真空包装和微波杀菌,在36℃条件下,至少可保藏11d,其微生物、感官指标均在国标允许范围内,可以有效延长鸡肉的保质期。

  1. 几种主要植物性熟食货架期的调查和分析%Major effect factor and solution research about several kinds of main vegetative prepared food

    Institute of Scientific and Technical Information of China (English)

    申可佳; 胡建中; 谭周进

    2009-01-01

    具有湘菜特色的预包装熟食制品自问世以来深受好评,风靡省内外.但是大部分熟食企业都是手工作坊,技术含量低,生产不规范,面对国家对食品保质期日益严格的要求,一些熟食企业便采取超标添加防腐剂来应付.所以,对延长熟食货架寿命的研究有着重要的意义.本文结合强制性地方标准中的一些与熟食保质期有关的要求对目前市场上一些主要熟食生产厂家生产的植物性熟食的保质期长短、灭菌措施、使用的防腐剂、维护措施等多方面做了调查及分析.最后提出自己的建议和看法.%High praise was given to the pre-packing prepared food product since it appeared,and the prepared food product is all around Hunan province.But the majority of prepared foods enterprise are the manual workshop.And the technical content is low,guarantees the nature time day by day strict request facing the country to food,some prepared food enterprises then adopt exceed the allowed figure the increase antiseptic to deal with.Therefore,to lengthens a prepared food goods shelf-life the research to have the vital significance.This article unified the Hunan Prepared food in compulsory place standard some to guarantee the nature time related request with the prepared food some main prepared foods to produce the factory production to the present market in the vegetative prepared food to guarantee the nature time length,the antiseptic measure,the use antiseptic,the maintenance measure and so on has variously made the investigation and the analysis.Finally puts forward own proposal and the view.

  2. 燃煤电厂脱硫烟囱防腐技术创新和应用探讨%Discussion on technology innovation of coal -fired power plant desulfurization chimney corrosion and its application

    Institute of Scientific and Technical Information of China (English)

    张庆虎; 吴金土

    2015-01-01

    Through the analysis of the existing coal-fired power plant desulfurization chimney corrosion technolo-gy, summed up the basic reason desulfurization chimney corrosion failures, technological innovation ideas pro-posed chimney desulfurization corrosion.And briefly describes the use of self-vulcanized butyl rubber anti-corrosion lining for coal-fired power plant desulfurization chimney antiseptic feasibility and success stories.%通过对现行燃煤电厂脱硫烟囱防腐技术分析,总结了脱硫烟囱防腐失效的原因,提出了脱硫烟囱防腐的技术创新思路。简要介绍了采用自硫化丁基橡胶防腐衬里对燃煤电厂脱硫烟囱进行防腐的可行性和成功案例。

  3. Difficult to control atopic dermatitis.

    Science.gov (United States)

    Darsow, Ulf; Wollenberg, Andreas; Simon, Dagmar; Taïeb, Alain; Werfel, Thomas; Oranje, Arnold; Gelmetti, Carlo; Svensson, Ake; Deleuran, Mette; Calza, Anne-Marie; Giusti, Francesca; Lübbe, Jann; Seidenari, Stefania; Ring, Johannes

    2013-01-01

    Difficult to control atopic dermatitis (AD) presents a therapeutic challenge and often requires combinations of topical and systemic treatment. Anti-inflammatory treatment of severe AD most commonly includes topical glucocorticosteroids and topical calcineurin antagonists used for exacerbation management and more recently for proactive therapy in selected cases. Topical corticosteroids remain the mainstay of therapy, the topical calcineurin inhibitors tacrolimus and pimecrolimus are preferred in certain locations. Systemic anti-inflammatory treatment is an option for severe refractory cases. Microbial colonization and superinfection contribute to disease exacerbation and thus justify additional antimicrobial / antiseptic treatment. Systemic antihistamines (H1) may relieve pruritus but do not have sufficient effect on eczema. Adjuvant therapy includes UV irradiation preferably of UVA1 wavelength. "Eczema school" educational programs have been proven to be helpful. PMID:23663504

  4. Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria.

    Science.gov (United States)

    Fani, Mohammadmehdi; Kohanteb, Jamshid

    2012-03-01

    Aloe vera is a medicinal plant with anti-inflammatory, antimicrobial, antidiabetic and immune-boosting properties. In the present study we investigated the inhibitory activities of Aloe vera gel on some cariogenic (Streptococcus mutans), periodontopathic (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis) and an opportunistic periodontopathogen (Bacteroides fragilis) isolated from patients with dental caries and periodontal diseases. Twenty isolates of each of these bacteria were investigated for their sensitivity to Aloe vera gel using the disk diffusion and microdilution methods. S. mutans was the species most sensitive to Aloe vera gel with a MIC of 12.5 µg/ml, while A. actinomycetemcomitans, P. gingivalis, and B. fragilis were less sensitive, with a MIC of 25-50 µg/ml (P Aloe vera gel at optimum concentration could be used as an antiseptic for prevention of dental caries and periodontal diseases.

  5. Aqueous dispersions of oxide nanoparticles as a treatment for pyoinflammatory diseases with chronic component

    International Nuclear Information System (INIS)

    Promising direction of surgery related to the treatment of acute purulent wounds with chronic component could be utilization of aqueous dispersions of nanostructures (ADN) produced by pulsed electric discharge in water. The investigation is addressed to finding out the opportunity of usage of an ADN for treatment of purulent wounds with a chronic component and comparison of its efficiency with the widespread antiseptics. For realization of investigation was used ADN, which has maximal share of 'small' nanostructures (<100 nm) with the greatest surface electric charge. High activity of reparative processes is established at use of ADN and subsequent moderate changes of the further healing. The attributes of cellular atypia and preternatural representations about inflammatory reactions are not revealed at local use of ADN.

  6. Malodorous oozing lesions after local cancer treatment - ethacridine powder prevents local infection

    Directory of Open Access Journals (Sweden)

    Gerhard Dyckhoff

    2011-08-01

    Full Text Available A common problem in local cancer treatment is infection of the resulting superficial tumour necrosis. The malodorous oozing lesions not only severely interfere with the patient’s quality of life but may also result in serious sequelae such as accelerated wasting, sepsis, and death. We developed a new formulation of the well-known antiseptic drug Ethacridine lactate in powder form to prevent such infections in local antineoplastic therapy. In a pilot study, the powder was applied in four patients receiving either an intralesional treatment with a platinum-based gel in the course of a study or photodynamic therapy. In all four patients with cervical respectively facial wounds with a surface of up to 80 cm2 a virtually dry and odorless scabby lesion resulted with no signs of infection. These initial clinical experiences suggest that Ethacridine lactate powder may be of benefit in the prevention of infection in palliative intratumoural cancer treatment.

  7. Determination of teat dip germicidal activity using the excised teat model.

    Science.gov (United States)

    Watts, J L; Boddie, R L; Owens, W E; Nickerson, S C

    1988-01-01

    Fifteen postmilking teat antiseptics were evaluated in seven trials using the excised teat model. All products were tested against Staphylococcus aureus Newbould 305 and Streptococcus agalactiae Cornell 48. Eight of nine iodophor products provided log reductions greater than 3 against Staph. aureus and Strep. agalactiae. A .045% iodophor product was ineffective. A .5% chlorhexidine product yielded a log reduction of 3.22 against Staph. aureus but only 2.92 against Strep. agalactiae. Two products containing fermented milk emulsified in detergent were tested upon receipt and after 1 yr storage. Fresh product provided log reductions greater than 3 against both pathogens. Storage for 1 yr resulted in minimal loss of germicidal activity. Three products containing 1, .5, and .2% dodecylaminoalkyl glycine provided log reductions greater than 3.5 against Staph. aureus and greater than 4 log reduction against Strep. agalactiae. PMID:3286695

  8. EVALUATION OF ANTIMICROBIAL ACTIVITY OF THE FRUITS OF CUCUMIS TRIGONUS ROXB.

    Directory of Open Access Journals (Sweden)

    S. Gopalakrishnan

    2012-05-01

    Full Text Available The antibacterial and antifungal activities of the successive extracts (petroleum ether (40-60ºC, benzene, chloroform, ethanol and water of the fruit of Cucumis trigonus Roxb. (Fam. Cucurbitaceae have been carried out against three gram positive bacteria, Bacillus cereus, Baccillus subtilis, Streptococcus faecalis, three gram negative bacteria, Pseudomonas aeruginosa, Klebsiella aerogenos, Proteaus vulgaris and two fungi, Candida albicans, Asparagillus flavans by using disk diffusion method. Zones of inhibition of the extracts have been compared with that of the standard antibiotics. The petroleum ether (40-60ºC and chloroform extracts showed no activity while the ethanolic extract showed more activity than the benzene and aqueous extracts. Minimum inhibitory concentration (MIC for the ethanolic extract of the fruit of Cucumis trigonus has also been determined. The results indicate that Cucumis trigonus is a potential antiseptic for the prevention and treatment of microbial infections.

  9. A Therapeutic Approach for Wound Healing by Using Essential Oils of Cupressus and Juniperus Species Growing in Turkey

    Directory of Open Access Journals (Sweden)

    Ibrahim Tumen

    2012-01-01

    Full Text Available Juniperus and Cupressus genera are mainly used as diuretic, stimulant, and antiseptic, for common cold and wound healing in Turkish folk medicine. In the present study, essential oils obtained from cones of Cupressus and berries of Juniperus were evaluated for their wound healing and anti-inflammatory effects. In vivo wound healing activity was evaluated by linear incision and circular excision experimental wound models, assessment of hydroxyproline content, and subsequently histopathological analysis. The healing potential was comparatively assessed with a reference ointment Madecassol. Additionally acetic-acid-induced capillary permeability test was used for the oils' anti-inflammatory activity. The essential oils of J. oxycedrus subsp. oxycedrus and J. phoenicea demonstrated the highest activities, while the rest of the species did not show any significant wound healing effect. The experimental study revealed that J. oxycedrus subsp. oxycedrus and J. phoenicea display remarkable wound healing and anti-inflammatory activities, which support the folkloric use of the plants.

  10. Alkaline ionic liquids applied in supported ionic liquid catalyst for selective hydrogenation of citral to citronellal

    Science.gov (United States)

    Salminen, Eero; Virtanen, Pasi; Mikkola, Jyri-Pekka

    2014-02-01

    The challenge in preparation of ionic liquids containing a strong alkaline anion is to identify a suitable cation which can tolerate the harsh conditions induced by the anion. In this study, a commercial quaternary ammonium compound (quat) benzalkonium [ADBA] (alkyldimethylbenzylammonium) was used as a cation in the synthesis of different alkaline ionic liquids. In fact, the precursor, benzalkonium chloride, is a mixture of alkyldimethylbenzylammonium chlorides of various alkyl chain lengths and is commonly used in the formulation of various antiseptic products. The prepared ionic liquids were utilized as Supported Ionic Liquid Catalysts (SILCAs). Typically, a SILCA contains metal nanoparticles, enzymes or metal complexes in an ionic liquid layer which is immobilized on a solid carrier material such as an active carbon cloth (ACC). The catalysts were applied in the selective hydrogenation of citral to citronellal which is an important perfumery chemical. Interestingly, 70 % molar yield towards citronellal was achieved over a catalyst containing the alkaline ionic liquid benzalkonium methoxide.

  11. Oil Essential Mouthwashes Antibacterial Activity against Aggregatibacter actinomycetemcomitans: A Comparison between Antibiofilm and Antiplanktonic Effects

    Directory of Open Access Journals (Sweden)

    Matteo Erriu

    2013-01-01

    Full Text Available The aim of this work is to determine the antibacterial activity of three marketed mouthwashes on suspended and sessile states of Aggregatibacter actinomycetemcomitans. The efficacy of two commonly used products in clinical practice, containing essential oils as active ingredients (menthol, thymol, methyl salicylate, and eucalyptol in association with or without alcohol, has been evaluated in comparison with a chlorhexidine-based mouthwash. The microtiter plate assay, in order to obtain a spectrophotometric measurement of bacterial responses at growing dilutions of each antiseptic, was used for the study. The analysis revealed that a good antibacterial activity is reached when the abovementioned mouthwashes were used at concentration over a 1/24 dilution and after an exposure time of 30 seconds at least. In conclusion, the alcoholic mouthwash appears to have a better biofilm inhibition than its antiplanktonic activity while the nonalcoholic product demonstrates an opposite effect with a better antiplanktonic behavior.

  12. Chemical composition and antimicrobial activity of peppermint (Mentha piperita L. Essential oil

    Directory of Open Access Journals (Sweden)

    Mohaddese Mahboubi

    2014-02-01

    Full Text Available Peppermint with antiseptic and known healing properties is a plant from the Labiatae family. In this study, we analyzed the chemical composition of essential oil from the flowering aerial part of peppermint by GC and GC/MS. Its antimicrobial activity was evaluated against bacteria, fungi and yeast by micro broth dilution assay. The fractional inhibitory concentration (FIC and FIC Index (FICI and related isobologram curve were determined by check board micro titer assay. The results exhibited that the MIC, MLC value of peppermint oil against different kinds of microorganisms were in the range of 0.125-2 and 0.125- >64 µl/ml, respectively. Candida albicans was the most sensitive microorganism and Pseudomonas aeruginosa was the less sensitive ones. The oil showed synergistic activities with vancomycin, gentamycin, and amphotericin B with the FICI less of 0.5. This oil could be used as natural antibiotics and may decrease the effective dose of antibiotics.

  13. Chlorhexidine, A Medicine for all the Oral Diseases

    Directory of Open Access Journals (Sweden)

    Radhika Gupta

    2012-03-01

    Full Text Available Chlorhexidine is a bisbiguanide antiseptic. It is active against both Gram-positive and Gram-negative strains as well as fungi. It has bacteriostatic and bactericidal actions. Chlorhexidine has excellent antiplaque activity and unique property of substantivity. So it has got wide applications starting from maintaining oral hygiene pre surgically to post operative and also in physically and mentally handicapped patients. Chlorhexidine is now routinely used by clinicians when they treat patients with fixed appliances in orthodontia and maxillofacial surgeries. Chlorhexidine has been extensively used in various medical fields such as gynecology, urology and ophthalmology; also in disinfection of operation fields and treatment of burns. Its products are available in various forms like mouth rinses, gels, sprays, toothpastes and varnishes.

  14. Rheology and physical-chemical characteristics of the solutions of the medicines

    Science.gov (United States)

    Urakov, A.; Urakova, N.

    2015-04-01

    In the laboratory studied the dynamics of rheology of water solutions with plasma- inflammatory and antiseptic funds when mixing them with blood, plasma and pus under the influence of the following physical and chemical factors of local interaction: gravity, specific gravity, temperature, relative viscosity, internal pressure, sparkling water, total concentration of the ingredients, surface activity, volume of acid and osmotic activity of medicines. Found that the rheology of biological liquids improve hyperthermic, highly alkaline and highly carbonated solution medicines. For the dilution of pus, dense festering mass of sulfur plugs and tear stones invited to apply heated to +39 - +42°C with aqueous solution of 0.5 - 3% hydrogen peroxide and 0.5 - 10% sodium bicarbonate saturated with carbon dioxide to excess pressure 0.2 ATM.

  15. Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies

    Science.gov (United States)

    Hayakumo, Sae; Arakawa, Shinichi; Takahashi, Masayoshi; Kondo, Keiko; Mano, Yoshihiro; Izumi, Yuichi

    2014-10-01

    The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were evaluated using in vitro time-kill assays. The cytotoxicity of NBW3 was evaluated using three-dimensional human buccal and gingival tissue models. The numbers of colony forming units (CFUs)/mL of P. gingivalis and A. actinomycetemcomitans exposed to NBW3 dropped to below the lower limit of detection (activity against representative periodontopathogenic bacteria and is not cytotoxic to cells of human oral tissues. The use of NBW3 as an adjunct to periodontal therapy would be promising.

  16. 植物精油的抑菌活性及其在食品贮藏中的应用

    Institute of Scientific and Technical Information of China (English)

    杨巍巍

    2014-01-01

    Essential oils, as the secondary plant metabolism substances, have strong biological activities and show good potential for application in antibacterial, antiseptic and other aspects. The domestic and overseas research of plant essential oils for antimicrobial activity is reviewed, and its application in food storage is briefly introduced.%植物精油具有很强的生物活性,作为植物代谢的次生物质,在抑菌、防腐等方面展现出很好的应用潜力。综述近年来国内外对于植物精油在抑菌方面的研究,及其在食品贮藏方面的应用性研究进展。

  17. New guаnidin-containing compounds with biocidal active: synthesis and prospects of study

    Directory of Open Access Journals (Sweden)

    Nadejda Kyznecova

    2014-04-01

    Full Text Available Synthesized a new complex compound-ditsitratoborat guanidinium studied its composition, structure , physical and chemical properties using IR spectroscopy , thermal , X-ray diffraction and elemental analysis , conductivity , quantum-chemical modeling . Proven biocidal activity against Escherichia coli, Staphylo-coccus aureus, yeasts bioassay methods . On the basis of silicate (clay and the Trans-Baikal Territory materials synthesized coordination compound obtained organic-inorganic composites in various ways (using mechanical activation and ultrasonic vibrations , studied its properties. The present invention extends the range of antiseptic preparations , the resulting composite is promising a wide range of applications due to their sorption properties and biocidal activity : qualitative improvement of natural , domestic, wastewater , swimming pools, reservoirs , soil objects .

  18. Antiproliferative and Molecular Mechanism of Eugenol-Induced Apoptosis in Cancer Cells

    Directory of Open Access Journals (Sweden)

    Eko Supriyanto

    2012-05-01

    Full Text Available Phenolic phytochemicals are a broad class of nutraceuticals found in plants which have been extensively researched by scientists for their health-promoting potential. One such a compound which has been comprehensively used is eugenol (4-allyl-2-methoxyphenol, which is the active component of Syzigium aromaticum (cloves. Aromatic plants like nutmeg, basil, cinnamon and bay leaves also contain eugenol. Eugenol has a wide range of applications like perfumeries, flavorings, essential oils and in medicine as a local antiseptic and anesthetic. Increasing volumes of literature showed eugenol possesses antioxidant, antimutagenic, antigenotoxic, anti-inflammatory and anticancer properties. Molecular mechanism of eugenol-induced apoptosis in melanoma, skin tumors, osteosarcoma, leukemia, gastric and mast cells has been well documented. This review article will highlight the antiproliferative activity and molecular mechanism of the eugenol induced apoptosis against the cancer cells and animal models.

  19. Scrum kidney: epidemic pyoderma caused by a nephritogenic Streptococcus pyogenes in a rugby team.

    Science.gov (United States)

    Ludlam, H; Cookson, B

    1986-08-01

    In December, 1984, an outbreak of pyoderma affected five scrum players in the St Thomas' Hospital rugby team. The causative organism, Streptococcus pyogenes, was acquired during a match against a team experiencing an outbreak of impetigo, and was transmitted to two front row players of another team a week later, and to two girlfriends of affected St Thomas' players a month later. The strain was M-type 49, tetracycline-resistant, and virulent. It caused salpingitis in a girlfriend and acute glomerulonephritis in one rugby player. No case of subclinical glomerulonephritis was detected in eight patients with pyoderma. Screening of the St Thomas' Hospital team revealed four further cases of non-streptococcal skin infection, with evidence for contemporaneous spread of Staphylococcus aureus. Teams should not field players with sepsis, and it may be advisable to apply a skin antiseptic to traumatised skin after the match. PMID:2874337

  20. Mouse bladder wall injection.

    Science.gov (United States)

    Fu, Chi-Ling; Apelo, Charity A; Torres, Baldemar; Thai, Kim H; Hsieh, Michael H

    2011-07-12

    Mouse bladder wall injection is a useful technique to orthotopically study bladder phenomena, including stem cell, smooth muscle, and cancer biology. Before starting injections, the surgical area must be cleaned with soap and water and antiseptic solution. Surgical equipment must be sterilized before use and between each animal. Each mouse is placed under inhaled isoflurane anesthesia (2-5% for induction, 1-3% for maintenance) and its bladder exposed by making a midline abdominal incision with scissors. If the bladder is full, it is partially decompressed by gentle squeezing between two fingers. The cell suspension of interest is intramurally injected into the wall of the bladder dome using a 29 or 30 gauge needle and 1 cc or smaller syringe. The wound is then closed using wound clips and the mouse allowed to recover on a warming pad. Bladder wall injection is a delicate microsurgical technique that can be mastered with practice.

  1. COMPARATIVE, QUALITATIVE AND QUANTITATIVE CHEMOTYPIC CHARACTERIZATION AMONG NORTH INDIAN TRIBULUS TERRESTRIS

    Directory of Open Access Journals (Sweden)

    Kumar Ashwani

    2012-06-01

    Full Text Available In India, Several medicinal plant species are used in herbal drug industries, whereas Tribulus terrestris extract has an ancient tradition in folk medicine and in ayurveda as a diuretic, mood enhancer, antiseptic and anti-inflammatory agent. Due to few phytochemical and pharmacological studies, there are no quality criteria for this Tribulus terrestris species as raw material. In this work, we present unique fingerprints of six samples of Tribulus terrestris population relating to the presence of flavonoids, alkaloid and saponins. Qualitative analysis of the phytochemicals of methanolic extracts revealed the presence of carbohydrates, saponins, phytosterols, phenols, flavonoids and tannins in all the plants. Quantitative analysis showed that the crude saponin was the major phytochemical constituent present in highest percentage followed by crude tannin in all six plants. These chemical characterizations can provide, for example, authentication of samples, detection of adulterations, and differentiation between closely related species.

  2. PICUNG (PANGIUM EDULE SEBAGAI BAHAN PENGAWET IKAN

    Directory of Open Access Journals (Sweden)

    Hermana Hermana

    2012-11-01

    Full Text Available (Pangium edule sebagai bahan pengawet ikan (Pitjung (Pangium edule as a preservative for fish. Pitjung (Pangium edule contains an antiseptic substance. It can be used as a preservative for fish in combination with salt. The self-life of fish will be increased if, in addition to 3% salt, certain amounts of pitjung are added. Sensory evaluation indicated that a combination of 6% pitjung with 3% salt could keep the fresh characteristics of fish for 6 days, therefore is useful to preserve fish during transport. Cara mengawet ikan seperti dilakukan orang di Labuan Jawa Barat, menggunakan picung (Pangium edule dan garam. Dengan cara ini garam yang digunakan lebih sedikit daripada yang diperlukan untuk mengasin. Picung terdapat di seluruh Indonesia, memungkinkan penggunaan cara ini di daerah-daerah penangkapan ikan yang kekurangan es atau garam. Penelitian ini bertujuan menilai kegunaan cara tersebut sehubungan dengan kesukaan konsumen.

  3. Development of bacterial resistance to biocides and antimicrobial agents as a consequence of biocide usage

    DEFF Research Database (Denmark)

    Seier-Petersen, Maria Amalie

    Biocides are chemical compounds with antimicrobial properties and they are widely used for disinfection, antiseptic and preservation purposes. Biocides have been applied for centuries due to early empirical approaches, such as cleansing of wounds with wine, vinegar and honey and salting of fish...... determined (Manuscript III). The biocides comprised benzalkonium chloride (BC), hydrogen peroxide (HP), sodium hypochlorite (SH), formaldehyde (FH), and caustic soda (NaOH). S. aureus isolates did in general not show reduced susceptibility to the biocides tested. However, a quaternary ammonium compound (QAC...... be of potential risk for human health, since these disinfectants are widely used at hospitals and in the food industry. Mobile genetic elements such as conjugative transposons are important vectors in the dissemination of antibiotic resistance determinants. Tn916 including the tetracycline resistance gene tet...

  4. Characterization of putative multidrug resistance transporters of the major facilitator-superfamily expressed in Salmonella Typhi

    DEFF Research Database (Denmark)

    Shaheen, Aqsa; Ismat, Fouzia; Iqbal, Mazhar;

    2015-01-01

    Multidrug resistance mediated by efflux pumps is a well-known phenomenon in infectious bacteria. Although much work has been carried out to characterize multidrug efflux pumps in Gram-negative and Gram-positive bacteria, such information is still lacking for many deadly pathogens. The aim......-hypersensitive Escherichia coli strain KAM42, and tested for transport of 25 antibacterial compounds, including representative antibiotics of various classes, antiseptics, dyes and detergents. Of the 15 tested putative transporters, STY0901, STY2458 and STY4874 exhibited a drug-resistance phenotype. Among these, STY4874...... conferred resistance to at least ten of the tested antimicrobials: ciprofloxacin, norfloxacin, levofloxacin, kanamycin, streptomycin, gentamycin, nalidixic acid, chloramphenicol, ethidium bromide, and acriflavine, including fluoroquinolone antibiotics, which were drugs of choice to treat S. Typhi infections...

  5. Lemon grass oil for improvement of oral health

    Directory of Open Access Journals (Sweden)

    Ruckmani Rajesvari

    2013-01-01

    Full Text Available Lemon grass essential oil has been used for decades to treat respiratory infections, sinusitis, bladder infections, high cholesterol, digestive problem, varicose veins and also for regeneration of connective tissue. It has anti spasmodic, anti-pyretic, anti-fungal, anti-bacterial, anti-inflammatory, anti-septic, insect repellent, sedative, vasodilator and flavoring properties. In china, it has been used traditionally as a remedy for stomach and liver diseases and also to treat rheumatism. Since lemon grass oil possess various pharmacological actions, it is also quite useful in dentistry. Hence, the objective of this article is to highlight various uses of lemon grass oil in the dental field and in the medical field in order to aid the professionals for future research.

  6. Nosocomial methicillin resistant staphylococcus aureus pneumonia - epidemiology and trends based on data of a network of 586 German ICUs (2005-2009

    Directory of Open Access Journals (Sweden)

    Meyer Elisabeth

    2010-11-01

    Full Text Available Abstract The epidemiology of MRSA pneumonia varies across countries. One of the most import risk factors for the development of nosocomial MRSA pneumonia is mechanical ventilation. Methicillin resistance in S. aureus ventilator associated pneumonia (VAP ranged between 37% in German, 54% in the US American and 78% in Asian and Latin American ICUs. In 2009, the incidence density of nosocomial VAP caused by MRSA was 0.28 per 1000 ventilation days in a network of 586 German ICUs. Incidences peaked in neurological and neurosurgical ICUs. Crude hospital mortality in studies performed after 2005 lay between 27% and 59% and attributable MRSA pneumonia mortality at 40%. Since 2005, US American and German data indicate decreasing trends for MRSA pneumonia. Measures to reduce MRSA pneumonia or to control the spread of MRSA include hand hygiene, standard and contact precautions, oral contamination with chlor hexidine, skin decontamination with antiseptics, screening, and (possibly patient isolation in a single room.

  7. 抗菌包装研究进展%Research Development of Antimicrobial Packaging

    Institute of Scientific and Technical Information of China (English)

    赵俊燕; 罗世永; 许文才

    2012-01-01

    总结了引起食品变质的原因,介绍了抗菌包装中所用抗菌剂,如无机抗菌剂、有机抗菌剂、天然抗菌剂、纳米抗菌剂和气体抗菌剂,简要描述了抗菌剂在食品包装中的应用进展。%The causes of food spoilage were summarized.The antimicrobial agents used in packaging were introduced,such as inorganic antiseptics,organic antibacterial agent,natural antimicrobials,nano antibacterial agent and gas antibacterial agent.The application progress of antibacterial agents in food packaging was described.

  8. Production of anticandidal cotton textiles treated with oak gall extract.

    Science.gov (United States)

    Tayel, Ahmed A; El-Tras, Wael F; Abdel-Monem, Omnia A; El-Sabbagh, Sabha M; Alsohim, Abdullah S; El-Refai, Elham M

    2013-01-01

    Candida albicans, one of the most dreadful fungal pathogens threatening humans, could not be easily prevented. The anticandidal activity of oak gall extract, Quercus infectoria (QIE), was investigated as a potential natural alternative to synthetic and chemical fungicides. QIE anticandidal potentiality was confirmed using both qualitative and quantitative assays. Cotton textiles were treated with QIE and then evaluated as anticandidal fabrics. QIE-treated textiles had a potent anticandidal activity, which could completely inhibit the inoculated C. albicans cells. The durability of anticandidal activity in QIE-treated textiles almost completely disappeared after the fourth laundering cycle. QIE could be recommended, however, as a potent anticandidal agent for preparing antiseptic solutions and emulsions and as a finishing agent for manufacturing anticandidal disposable diapers and hygienic clothes. PMID:24401783

  9. Herbs as new type of green inhibitors for acidic corrosion of steel

    Energy Technology Data Exchange (ETDEWEB)

    Khamis, E. [Faculty of Science, Chemistry Department, Alexandria University, P.O. Box 426 Ibrahimia Alexandria 21321 (Egypt); AlAndis, N. [College of Science, Chemistry Department, King Saud University (Saudi Arabia)

    2002-09-01

    Corrosion inhibition of steel in sulphuric acid by six different herb plants has been studied using a.c and d.c electrochemical techniques. The environmentally friendly investigated compounds are namely: thyme, coriander, hibiscus, anis, black cumin and Garden cress. Electrochemical impedance spectroscopy has been successfully used to evaluate the performance of these compounds. The ac measurements showed that the dissolution process is activation controlled. Bode and theta diagrams show only one time constant ({tau}). Potentiodynamic polarization curves indicate that the studied compounds are mixed-type inhibitors. The order of increasing inhibition efficiency was correlated with the change of the constituent active materials of the compounds. Thyme, which contains the powerful antiseptic thymol as the active ingredient, offers excellent protection for steel surface. (Abstract Copyright [2002], Wiley Periodicals, Inc.)

  10. Reconstruction of the pelvis and perineum with a free latissimus dorsi myocutaneous flap: a case report.

    LENUS (Irish Health Repository)

    Kieran, I

    2012-11-01

    Reconstruction of the perineum and pelvic cavity in continuity is an uncommon and difficult challenge. This case describes a 66-year-old man who presented following recurrence of a Dukes\\' B rectosigmoid adenocarcinoma that had been treated nine years previously by anterior resection, 5-fluorouracil and radiotherapy. His recurrent disease was treated with radical pelvic exenteration with formation of an end colostomy and urinary ileal conduit. A post-operative pelvic collection necessitated incisional drainage via the perineum. This resulted in a perineal defect in continuity with the pelvic cavity, neither of which healed in spite of alternate day packing with antiseptic dressings. The perineum and cavity were reconstructed successfully with a microvascular transfer of the latissimus dorsi using the primary gracilis pedicle as recipient donor vessels.

  11. Periimplantitis treatment: literature review

    Directory of Open Access Journals (Sweden)

    Leonardo FRANCIO

    2008-08-01

    Full Text Available Introduction and objective: The aim of this study was to review in theliterature the aim modalities of periimplantitis treatment. They were study the use of buccal antiseptics, antimicrobial treatment, open debridment,close debridment, osseous grafts, the use of membranes, combination with osseous grafts and membrane technique and laserteraphy.Literature review: The most of studies showed cases about these treatment types but only one related a specific protocol of peri-implantar disease. All of other demonstrated treatment associations had success on the most absolute cases, independent of treatment. Conclusion: The techniques association of treatment is valid and necessary, but to have a more specific conclusion should be done more studies in humans.

  12. The 'liaisons dangereuses' between iron and antibiotics.

    Science.gov (United States)

    Ezraty, Benjamin; Barras, Frédéric

    2016-05-01

    The decline in the rate of new antibiotic discovery is of growing concern, and new antibacterial strategies must now be explored. This review brings together research in two fields (metals in biology and antibiotics) in the hope that collaboration between scientists working in these two areas will lead to major advances in understanding and the development of new approaches to tackling microbial pathogens. Metals have been used as antiseptics for centuries. In this review, we focus on iron, an essential trace element that can nevertheless be toxic to bacteria. We review the many situations in which iron and antibiotics have combinatorial effects when used together. Understanding the molecular relationships between iron and antibiotics, from pure chemistry to gene reprogramming via biochemical competition, is important not only to increase basic knowledge, but also for the development of treatments against pathogens, with a view to optimizing antibiotic efficacy. PMID:26945776

  13. Slow Release of Nitric Oxide from Charged Catheters and Its Effect on Biofilm Formation by Escherichia coli▿

    Science.gov (United States)

    Regev-Shoshani, Gilly; Ko, Mary; Miller, Chris; Av-Gay, Yossef

    2010-01-01

    Catheter-associated urinary tract infection is the most prevalent cause of nosocomial infections. Bacteria associated with biofilm formation play a key role in the morbidity and pathogenesis of these infections. Nitric oxide (NO) is a naturally produced free radical with proven bactericidal effect. In this study, Foley urinary catheters were impregnated with gaseous NO. The catheters demonstrated slow release of nitric oxide over a 14-day period. The charged catheters were rendered antiseptic, and as such, were able to prevent bacterial colonization and biofilm formation on their luminal and exterior surfaces. In addition, we observed that NO-impregnated catheters were able to inhibit the growth of Escherichia coli within the surrounding media, demonstrating the ability to eradicate a bacterial concentration of up to 104 CFU/ml. PMID:19884372

  14. Slow release of nitric oxide from charged catheters and its effect on biofilm formation by Escherichia coli.

    Science.gov (United States)

    Regev-Shoshani, Gilly; Ko, Mary; Miller, Chris; Av-Gay, Yossef

    2010-01-01

    Catheter-associated urinary tract infection is the most prevalent cause of nosocomial infections. Bacteria associated with biofilm formation play a key role in the morbidity and pathogenesis of these infections. Nitric oxide (NO) is a naturally produced free radical with proven bactericidal effect. In this study, Foley urinary catheters were impregnated with gaseous NO. The catheters demonstrated slow release of nitric oxide over a 14-day period. The charged catheters were rendered antiseptic, and as such, were able to prevent bacterial colonization and biofilm formation on their luminal and exterior surfaces. In addition, we observed that NO-impregnated catheters were able to inhibit the growth of Escherichia coli within the surrounding media, demonstrating the ability to eradicate a bacterial concentration of up to 10(4) CFU/ml. PMID:19884372

  15. Research on Characteristics of Nisin and the Application in Food Industry%乳酸链球菌肽的特性及其在食品工业中的应用

    Institute of Scientific and Technical Information of China (English)

    孙兆竹; 申秋华; 白文杰

    2013-01-01

    乳酸链球菌素是由乳酸链球菌产生的一种高效、无毒、安全、无副作用的天然食品防腐剂.介绍了乳酸链球菌素(Nisin)的特性及其抑菌防腐机理,并就其在食品工业中的应用加以论述.%Nisin is a kind of high efficiency and non-poisonous effect natural bio-preservative food antiseptic produced by Lactococcus lactis.The characteristics and antibiotic mechanism of Nisin were introduced,as well as the application in food industry.

  16. Thiomersal-containing vaccines - a review of the current state of knowledge.

    Science.gov (United States)

    Gołoś, Aleksandra; Lutyńska, Anna

    2015-01-01

    Thiomersal is an organomercury compound known for its antiseptic and antifungal properties and used as an antibacterial agent in pharmaceutical products, including vaccines and other injectable biological products. In recent years, concerns about the possible link between immunization with thiomersal-containing vaccines and autism development have grown. Many case-control and cohort studies have been conducted on a number of populations, and none of them have confirmed the hypothetical relation between thiomersal and increased risk of autism spectrum disorders (ASDs) development. It is also confirmed by the fact, that since 1999, number of thiomersal-containing vaccines used worldwide is decreasing year by year, while the prevalence of ASDs cases is rising. There are no contraindications to the use of vaccines with thiomersal in infants, children and non-pregnant women. The risk of serious complications associated with the development of diseases in unvaccinated individuals far outweighs the potential risk of adverse consequences associated with immunization with thiomersal-containing vaccines.

  17. Aloe vera in dentistry.

    Science.gov (United States)

    Sujatha, G; Kumar, G Senthil; Muruganandan, J; Prasad, T Srinivasa

    2014-10-01

    Aloe vera is a medicinal plant which has been used for thousands of years. The health benefits of aloe vera is well known and the dental uses of this plant is multiple. Interest is gathering among researchers regarding the use of this plant. Studies have proved the antiseptic, anti inflammatory, antiviral and antifungal properties of aloe vera and the use of this plant is proved beneficial. This plant is proved to be non allergic and very good in building up the immune system. Aloe vera is gaining popularity in dentistry as it is completely natural and there is no side effects being reported with its use. This paper gives an overview of the uses of this miracle plant and its uses in dentistry.

  18. Joint-action of antifouling substances in copper-free paints.

    Science.gov (United States)

    Faÿ, Fabienne; Carteau, David; Linossier, Isabelle; Delbury, Maxime; Vallée-Réhel, Karine

    2013-02-01

    Due to the environmentally harmful impact of tributyltin self-polishing paints, there is a critical need of more ecological alternatives. The aim of the present work is to study the joint-action of three molecules chosen in order to combine the two modes of prevention: chemical and physical repelling of biofouling. This "hybrid" system is principally dedicated to disturb durable settlement of microfouling. Each component was chosen according to its specific properties: chlorhexidine is a bisdiguanide antiseptic with antibacterial activity, zinc peroxide is an inorganic precursor of high instable entities which react with seawater to create hydrogen peroxide, Tween 85 is a non ionic surfactant disturbing interactions between colonizing organisms and surface. Obtained results highlighted the interest on mixing such molecules to get additive action on antifouling efficiency. PMID:23104027

  19. Ulcerative Uremic Stomatitis - Review of the Literature and A Rare Case Report

    Directory of Open Access Journals (Sweden)

    Shantala Arunkumar

    2015-01-01

    Full Text Available Uremic Stomatitis (US represents a comparatively uncommon intraoral complication seen, mostly, in cases of end-stage renal disease or undiagnosed or untreated chronic renal failure. Its frequency has diminished due to the advent of renal dialysis. Clinically uremic stomatitis is characterized by the presence of painful plaques and crusts that are usually distributed on the buccal and labial mucosa, dorsal or ventral surface of the tongue, gingiva, and floor of the mouth. Ultimate treatment consists of improvement of blood urea concentration and underlying renal failure is supported by enhancement of oral hygiene with antiseptic mouthwashes and antimicrobial/antifungal agents, if necessary. Here we report a rare case of ulcerative type of uremic stomatitis occurring in a patient of chronic renal failure due to sudden relapse of uremia and reviewed the possible pathophysiology of oral symptoms of chronic renal failure.

  20. Intrinsic Antimicrobial Resistance Determinants in the Superbug Pseudomonas aeruginosa

    Science.gov (United States)

    Murray, Justine L.; Kwon, Taejoon; Marcotte, Edward M.

    2015-01-01

    ABSTRACT Antimicrobial-resistant bacteria pose a serious threat in the clinic. This is particularly true for opportunistic pathogens that possess high intrinsic resistance. Though many studies have focused on understanding the acquisition of bacterial resistance upon exposure to antimicrobials, the mechanisms controlling intrinsic resistance are not well understood. In this study, we subjected the model opportunistic superbug Pseudomonas aeruginosa to 14 antimicrobials under highly controlled conditions and assessed its response using expression- and fitness-based genomic approaches. Our results reveal that gene expression changes and mutant fitness in response to sub-MIC antimicrobials do not correlate on a genomewide scale, indicating that gene expression is not a good predictor of fitness determinants. In general, fewer fitness determinants were identified for antiseptics and disinfectants than for antibiotics. Analysis of gene expression and fitness data together allowed the prediction of antagonistic interactions between antimicrobials and insight into the molecular mechanisms controlling these interactions. PMID:26507235

  1. Being Lister: ethos and Victorian medical discourse.

    Science.gov (United States)

    Connor, J J; Connor, J T H

    2008-06-01

    Stylistic analysis and rhetorical theory are used in this study to inform our understanding of impediments to the successful uptake of a new medical idea. Through examination of the work of the Victorian surgeon Joseph Lister, who was described by one biographer as suffering from "stylistic ham-handedness", the study provides insights into the difficulty that Lister had in explaining his theory of antiseptic surgery. Using three comparisons-Lister's scientific style in public discourse with that of his students, and Lister's scientific style in private discourse with those of both a surbordinate and a superior-the study suggests that the rhetorical concept of ethos played a major role in his communication difficulties. In this way, it presents a more nuanced perspective on modern presentations of "model" communications versus communication failures: that is, that problematic written discourse offers as useful a heuristic device as does exemplary discourse.

  2. Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): a review of their traditional uses, phytochemistry, and pharmacology.

    Science.gov (United States)

    Bozorgi, Mahbubeh; Memariani, Zahra; Mobli, Masumeh; Salehi Surmaghi, Mohammad Hossein; Shams-Ardekani, Mohammad Reza; Rahimi, Roja

    2013-01-01

    Pistacia, a genus of flowering plants from the family Anacardiaceae, contains about twenty species, among them five are more popular including P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus. Different parts of these species have been used in traditional medicine for various purposes like tonic, aphrodisiac, antiseptic, antihypertensive and management of dental, gastrointestinal, liver, urinary tract, and respiratory tract disorders. Scientific findings also revealed the wide pharmacological activities from various parts of these species, such as antioxidant, antimicrobial, antiviral, anticholinesterase, anti-inflammatory, antinociceptive, antidiabetic, antitumor, antihyperlipidemic, antiatherosclerotic, and hepatoprotective activities and also their beneficial effects in gastrointestinal disorders. Various types of phytochemical constituents like terpenoids, phenolic compounds, fatty acids, and sterols have also been isolated and identified from different parts of Pistacia species. The present review summarizes comprehensive information concerning ethnomedicinal uses, phytochemistry, and pharmacological activities of the five mentioned Pistacia species. PMID:24453812

  3. HPLC analysis of diosgenin in three species of Costus

    Directory of Open Access Journals (Sweden)

    G.Sulakshana

    2014-11-01

    Full Text Available Medicinal plants form an essential part of indigenous pharmaceutical system. Costus species (Family: Costaceae, commonly called as spiral ginger or crepe ginger are important medicinal plants used in traditional system of medicine in India. These plants are used for their stimulant, carminative, diuretic, digestive and antiseptic properties. Some of the species of Costus are mainly used for treating diabetes. Species of Costus are known to contain a steroidal saponin- diosgenin as a major bioactive component, which is utilized as a precursor for the synthesis of various drugs. The present study is taken up to quantify the amount of diosgenin present in rhizomes and leaves of three species of Costus (C.pictus, C.speciosus and C.igneus using HPLC analysis.

  4. PREPARATION AND PROPERTIES OF THE COLLODIAL SOLUTION BASED ON BIOGENIC METAL NANOPARTICLES

    Directory of Open Access Journals (Sweden)

    K. V. Liapina

    2014-12-01

    Full Text Available The aim of the work was obtaining a stable suspension based on biocompatible substances with application of biogenic metal nanoparticles encapsulated into NaCl salt matrix, as a precursor. Water-soluble complex based on different amine derivatives with antiseptic properties was selected as a liquid for salt dissolution. The solution was subjected to dispersion using ultrasonication at elevated temperature. Dispersion is accompanied by salt shell removal with simultaneous formation of an organic shell on the surfaces of metal nanoparticles that ensure their stabilization. Study of the suspension after soaking at room temperature for 100 days showed that its characteristics remain stable. A method for producing a stable colloidal solution based on nanoparticles of biogenic metal (Cu, Co, fem etc. was developed. Metal nanopowder encapsulated into salt shell was used as a precursor. It is shown that such colloidal solutions are characterized by narrow size dispersion, as well as stability to temperature impact and time factor.

  5. Influence of subinhibitory concentrations of antimicrobials on hydrophobicity, adherence and ultra-structure of Fusobacterium nucleatum

    Directory of Open Access Journals (Sweden)

    Okamoto Ana C.

    2002-01-01

    Full Text Available Fusobacterium nucleatum is considered a bridge organism between earlier and later colonizers in dental biofilms and a putative periodontopathogen. In Dentistry, antimicrobial agents are used for treatment and control of infectious diseases associated with dental plaque. Antiseptics have been used in association with antibiotics to reduce infections after oral surgeries. In this study, the influence of subinhibitory concentrations (SC of chlorhexidine, triclosan, penicillin G and metronidazole, on hydrophobicity, adherence to oral epithelial cells, and ultra-structure of F. nucleatum was examined. All isolates were susceptible to chlorhexidine, triclosan, and metronidazole; however, most of the isolates were susceptible to penicillin G, and all of them were hydrophilic when grown with or without antimicrobials. Adherence was decreased by all antimicrobials. Results suggest that adherence of F. nucleatum was influenced by adhesins because structures such as fimbries or capsule were not observed by transmission electronic microscope.

  6. Aqueous dispersions of oxide nanoparticles as a treatment for pyoinflammatory diseases with chronic component

    Energy Technology Data Exchange (ETDEWEB)

    Rutberg, Ph; Kolikov, V; Snetov, V; Stogov, A [Institute for Electrophysics and Electric Power Russian Academy of Sciences, 18 Dvortsovaya nab., St.-Petersburg, 191186 (Russian Federation); Moshkin, A; Khalilov, M, E-mail: Stogov2007@yandex.ru [Oryol State University, Medical Institute, October st. 25, Oryol, 302028 (Russian Federation)

    2011-04-01

    Promising direction of surgery related to the treatment of acute purulent wounds with chronic component could be utilization of aqueous dispersions of nanostructures (ADN) produced by pulsed electric discharge in water. The investigation is addressed to finding out the opportunity of usage of an ADN for treatment of purulent wounds with a chronic component and comparison of its efficiency with the widespread antiseptics. For realization of investigation was used ADN, which has maximal share of 'small' nanostructures (<100 nm) with the greatest surface electric charge. High activity of reparative processes is established at use of ADN and subsequent moderate changes of the further healing. The attributes of cellular atypia and preternatural representations about inflammatory reactions are not revealed at local use of ADN.

  7. [Pharmaceutical history of capuchin monastery in Prague-Hradčan Part II. Capuchin balsam (Balsamum capucinorum)].

    Science.gov (United States)

    Nesměrák, Karel; Kunešová, Jana

    2015-06-01

    The history of traditional capuchin balsam is the focal point of the second part of the article on the unknown history of pharmacy at the capuchin monastery in Prague-Hradčany. Capuchin balsam, a medicinal speciality, was being manufactured in the monastery from the end of the 18th century till the year 1950. It is a spirit tincture, its prescription originating from the formulation by Oswald Croll. Balsamum Peruvianum, Gummiresina myrrha, Gummiresina olibanum, and Styrax are the main ingredients, besides assorted plants. The balsam was taken as an antiseptic, antiphlogistic, and analgesic. The balsam was a favoured rustic medicine, and it was sold also abroad (Germany, Poland, USA, Ireland, Belgium). The profit made from the sale of the balsam supported the reconstruction and the maintenance of the monastery and the local theological studies. Other medical formulations connected with the name of the capuchin order are also mentioned.Key words: pharmaceutical history capuchins capuchin balsam monastics pharmacies.

  8. Postradiation therapy skin complications and aspects of their treatment

    International Nuclear Information System (INIS)

    X-ray is a common therapy for malignant skin cancers. 60-70 Gy is the necessary therapeutic dose for squamous cell carcinoma. Early and late postradiation complications are X-ray dermatitis, erosions, ulcers and atrophies. The therapy for complications includes topical antiseptics and antibacterial agents, debridement, dressing, and even plastic and reconstructive surgery. Capillary regrowth, immunosuppression, local ischemia and epithelialisation are to be considered. The aim of this study was to observe and compare the topical agents in treatment of postradiation skin compilations. Early compilations were treated effectively using Diaethonum ointment (prophylaxis and therapy), Fucicort cream, Linoladiol-HN cream. With late complications Chlorhexidine gluconate 0.05% solution, Iruxol and Desitin ointments showed good results, dealing with trophical ulcers. There is no universal drug for treating X-ray caused complications, in every case the therapy is to be considered individually. (authors)

  9. Bioactive Coatings for Orthopaedic Implants—Recent Trends in Development of Implant Coatings

    Directory of Open Access Journals (Sweden)

    Bill G. X. Zhang

    2014-07-01

    Full Text Available Joint replacement is a major orthopaedic procedure used to treat joint osteoarthritis. Aseptic loosening and infection are the two most significant causes of prosthetic implant failure. The ideal implant should be able to promote osteointegration, deter bacterial adhesion and minimize prosthetic infection. Recent developments in material science and cell biology have seen the development of new orthopaedic implant coatings to address these issues. Coatings consisting of bioceramics, extracellular matrix proteins, biological peptides or growth factors impart bioactivity and biocompatibility to the metallic surface of conventional orthopaedic prosthesis that promote bone ingrowth and differentiation of stem cells into osteoblasts leading to enhanced osteointegration of the implant. Furthermore, coatings such as silver, nitric oxide, antibiotics, antiseptics and antimicrobial peptides with anti-microbial properties have also been developed, which show promise in reducing bacterial adhesion and prosthetic infections. This review summarizes some of the recent developments in coatings for orthopaedic implants.

  10. COMPLICATIONS AFTER EXTRACTION OF IMPACTED THIRD MOLARS - LITERATURE REVIEW

    Directory of Open Access Journals (Sweden)

    Elitsa G. Deliverska

    2016-07-01

    Full Text Available Third molar surgery is the most common procedure performed by oral and maxillofacial surgeons worldwide. This article addresses the incidence of specific complications and, where possible, offers a preventive or management strategy. Complications, such as pain, dry socket, swelling, paresthesia of the lingual or inferior alveolar nerve, bleeding, and infection are most common. Factors thought to influence the incidence of complications after third molar removal include age, gender, medical history, oral contraceptives, presence of pericoronitis, poor oral hygiene, smoking, type of impaction, relationship of third molar to the inferior alveolar nerve, surgical time, surgical technique, surgeon experience, use of perioperative antibiotics, use of topical antiseptics, use of intra-socket medications, and anaesthetic technique. For the general dental practitioner, as well as the oral and maxillofacial surgeon, it is important to be familiar with all the possible complications after this procedure. This improves patient education and leads to prevention, early recognition and management.

  11. Hautreizungen durch Propylenglykol

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Storrs, F J

    1982-01-01

    Propylene glycol is used in foods, medicine and cosmetics because it is a good solvent which has, simultaneously, moisture-regulating, antiseptic, and preservative effects. Propylene glycol may produce eczematous skin reactions of toxic and, more rarely, of allergic nature. Positive patch test...... reactions to propylene glycol are difficult to interpret. Allergic reactions may be confirmed by a clear clinical relevance, repeated local skin provocation (usage test), or oral provocation. In the Department of Dermatology, University of Oregon, 84 patients were patch tested with 100% propylene glycol....... Five of 12 patch test-positive patients had allergic reactions while seven had irritant reactions. In the Department of Dermatology, Gentofte Hospital, 248 consecutive eczema patients were patch-tested with propylene glycol in concentrations of 100%, 20%, and 2% in water. Two of five patients...

  12. How World War 1 changed global attitudes to war and infectious diseases.

    Science.gov (United States)

    Shanks, G Dennis

    2014-11-01

    World War 1 was a key transition point towards scientific medicine. Medical officers incorporated Louis Pasteur's discoveries into their understanding of microorganisms as the cause of infectious diseases, which were therefore susceptible to rational control and treatment measures even in the pre-antibiotic era. Typhoid vaccination led to the successful evasion of the disastrous epidemics of previous wars. The incidence of tetanus was probably decreased by giving millions of doses of horse antitoxin to wounded soldiers. Quinine treated but could not control malaria; its use required mass compulsion. Tuberculosis was not a great military problem during World War 1, although mortality in civilian populations increased substantially. Treatment of sexually transmitted infections remained a matter of aversive conditioning, with invasive antiseptics used in the absence of antibiotics. Pandemic influenza in 1918-19 killed more people than died during the entire war, showing how much remained beyond the capability of the scientists and doctors who fought infectious diseases during World War 1.

  13. Allergy to Chlorhexidine.

    Science.gov (United States)

    Pemberton, Michael N

    2016-04-01

    Chlorhexidine is an effective antiseptic which is widely used in dentistry. Over recent years, it has also been used in other healthcare products as well as in cosmetics. Anaphylaxis to chlorhexidine has been increasingly reported throughout the world, including two incidents in the UK where chlorhexidine-containing mouthwash had been used to wash tooth sockets following recent tooth extraction. Chlorhexidine is under-recognized as a cause of anaphylaxis and dentists should be aware of its potential for serious adverse effects. Dentists need to consider whether the washing out of a tooth socket with chlorhexidine solution should be avoided in the treatment of established dry socket. On current evidence the potential risks of using chlorhexidine as irrigation solution for treating an established dry socket appears to outweigh any known benefit. CPD/Clinical Relevance: Chlorhexidine has the potential to cause anaphylaxis in the dental surgery. PMID:27439274

  14. Alkaline ionic liquids applied in supported ionic liquid catalyst for selective hydrogenation of citral to citronellal

    Directory of Open Access Journals (Sweden)

    Eero eSalminen

    2014-02-01

    Full Text Available The challenge in preparation of ionic liquids containing a strong alkaline anion is to identify a suitable cation which can tolerate the harsh conditions induced by the anion. In this study, a commercial quaternary ammonium compound (quat benzalkonium [ADBA] (alkyldimethylbenzylammonium was used as a cation in the synthesis of different alkaline ionic liquids. In fact, the precursor, benzalkonium chloride, is a mixture of alkyldimethylbenzylammonium chlorides of various alkyl chain lengths and is commonly used in the formulation of various antiseptic products. The prepared ionic liquids were utilized as Supported Ionic Liquid Catalysts (SILCAs. Typically, a SILCA contains metal nanoparticles, enzymes or metal complexes in an ionic liquid layer which is immobilized on a solid carrier material such as an active carbon cloth (ACC. The catalysts were applied in the selective hydrogenation of citral to citronellal which is an important perfumery chemical. Interestingly, 70 % molar yield towards citronellal was achieved over a catalyst containing the alkaline ionic liquid benzalkonium methoxide.

  15. A NEW VARIETY OF ORIGANUM VULGARE L. – DENIS, CREATED AT VRDS BACĂU IN ECOLOGIC AGRICULTURE CONDITION, CERTIFIED IN 2007 YEAR

    Directory of Open Access Journals (Sweden)

    FALTICEANU MARCELA

    2008-11-01

    Full Text Available Oregano is a perennial plant of 0,6 – 0,8m high. The flowers are small, coloured in red till lilac-lavender. It blossom from July till September, being pollinated by bees. The utility rate of plants is 3 : 5.In the literature is mentioned as a plants with multiple uses: culinary (as a condiment plant or for the preparation of a aromatised tea, the leaves can be consumed fresh or cooked; ornamental (is decorative through port, bush and flowers: often is cultivated in pots; medicinal (is has an antiseptically and expectorant effects, being used also in affections of respiratory systems, indigestions, arthritis, aromatherapy etc; melliferous (is a good melliferous plant; in biologic agriculture (with repellent effect for insects, is recommended for association with many vegetable species, also because the plants cover very well the soil, thus providing an herbicide effect; cosmetics (perfume, soap, spay industry.

  16. Potassium permanganate ingestion as a suicide attempt

    Directory of Open Access Journals (Sweden)

    Tuba Cimilli Ozturk

    2012-01-01

    Full Text Available Potassium permanganate is a highly corrosive, water-soluble oxidizing antiseptic. A 68- year-old female patient was admitted to our Emergency Department after ingestion of 3 tablets of 250 mg potassium permanganate as a suicide attempt. The physical exam revealed brown stained lesions in the oropharynx. Emergency endoscopy was performed by the gastroenterologist after the third hour of ingestion. Emergency endoscopy revealed multiple superficial (Grade I-II lesions on the esophagus and cardia, which were considered secondary to the caustic substance. The mainstay in the treatment of potassium permanganate is supportive and the immediate priority is to secure the airway. Emergency endoscopy is an important tool used to evaluate the location and severity of injury to the esophagus, stomach and duodenum after caustic ingestion. Patients with signs and symptoms of intentional ingestion should undergo endoscopy within 12 to 24 h to define the extent of the disease.

  17. Progress of Composition and Function of Black Garlic%黑蒜成分及功能的研究进展

    Institute of Scientific and Technical Information of China (English)

    安东; 李新胜; 马超; 周萍; 葛邦国; 王朝川; 孟晓峰

    2014-01-01

    We summarized main chemical composition of black garlic and biological functions of antioxidant, antiseptic, anti-aging, enhance immunity, anticancer and blood sugar regulation in this paper. Health promoting benefits of black garlic as a healthy food to human were introduced, and the direction for future research was proposed as well.%本文总结了黑蒜的主要化学成分以及其抗氧化作用、杀菌、抗衰老、增强免疫力、抗癌和调节血糖等生物学功能,介绍了黑蒜作为健康食品对人机体的积极作用,提出了今后的研究方向。

  18. An FDA-Drug Library Screen for Compounds with Bioactivities against Meticillin-Resistant Staphylococcus aureus (MRSA

    Directory of Open Access Journals (Sweden)

    Qiu Ying Lau

    2015-10-01

    Full Text Available The lack of new antibacterial drugs entering the market and their misuse have resulted in the emergence of drug-resistant bacteria, posing a major health crisis worldwide. In particular, meticillin-resistant Staphylococcus aureus (MRSA, a pathogen responsible for numerous human infections, has become endemic in hospitals worldwide. Drug repurposing, the finding of new therapeutic indications for approved drugs, is deemed a plausible solution to accelerate drug discovery and development in this area. Towards this end, we screened 1163 drugs approved by the Food and Drug Administration (FDA for bioactivities against MRSA in a 10 μM single-point assay. After excluding known antibiotics and antiseptics, six compounds were identified and their MICs were determined against a panel of clinical MRSA strains. A toxicity assay using human keratinocytes was also conducted to gauge their potential for repurposing as topical agents for treating MRSA skin infections.

  19. Acute and Chronic Toxicity of an Aqueous Fraction of the Stem Bark of Stryphnodendron adstringens (Barbatimão) in Rodents.

    Science.gov (United States)

    Costa, Marco Antonio; Palazzo de Mello, João Carlos; Kaneshima, Edílson Nobuyoshi; Ueda-Nakamura, Tânia; Dias Filho, Benedito Prado; Audi, Elisabeth Aparecida; Nakamura, Celso Vataru

    2013-01-01

    Stryphnodendron adstringens has a high tannin content and is used as an antiseptic and antimicrobial and in the treatment of leucorrhea, gonorrhea, wound healing, and gastritis. The present study evaluated the toxic effects of the heptamer prodelphinidin (F2) from the stem bark of S. adstringens in rodents. In the acute toxicity test, the mice that received oral doses exhibited reversible effects, with an LD50 of 3.015 mg · kg(-1). In the chronic toxicity test at 90 days, Wistar rats were treated with different doses of F2 (10, 100, and 200 mg · kg(-1)). In the biochemical, hematological, and histopathological examinations and open-field test, the different dose groups did not exhibit significant differences compared with controls. The present results indicate that F2 from the stem bark of S. adstringens caused no toxicity with acute and chronic oral treatment in rodents at the doses administered.

  20. Acute and Chronic Toxicity of an Aqueous Fraction of the Stem Bark of Stryphnodendron adstringens (Barbatimão in Rodents

    Directory of Open Access Journals (Sweden)

    Marco Antonio Costa

    2013-01-01

    Full Text Available Stryphnodendron adstringens has a high tannin content and is used as an antiseptic and antimicrobial and in the treatment of leucorrhea, gonorrhea, wound healing, and gastritis. The present study evaluated the toxic effects of the heptamer prodelphinidin (F2 from the stem bark of S. adstringens in rodents. In the acute toxicity test, the mice that received oral doses exhibited reversible effects, with an LD50 of 3.015 mg·kg−1. In the chronic toxicity test at 90 days, Wistar rats were treated with different doses of F2 (10, 100, and 200 mg·kg−1. In the biochemical, hematological, and histopathological examinations and open-field test, the different dose groups did not exhibit significant differences compared with controls. The present results indicate that F2 from the stem bark of S. adstringens caused no toxicity with acute and chronic oral treatment in rodents at the doses administered.