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Sample records for anticuerpos anti hbs

  1. Anti-HBs antibody of normal human subjects predominantly binds 54 K and 60 K dalton HBs polypeptides.

    OpenAIRE

    Ono,Ryosaku; Koide, Norio; Nagashima,Hideo

    1986-01-01

    The structure of hepatitis B virus surface antigen (HBs) recognized by anti-HBs antibody was analyzed by western blotting using anti-HBs sera obtained from normal subjects, from rabbits immunized with purified HBs and commercially available goat serum. The HBs used had 7 components of 24 K, 27 K, 33 K, 36 K, 39 K, 43 K and 67-72 K daltons. Goat anti-HBs serum bound all of these components, while human and rabbit anti-HBs sera bound only two components (60 K and 54 K daltons), which were hardl...

  2. Anti-HBs antibody of normal human subjects predominantly binds 54 K and 60 K dalton HBs polypeptides.

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    Ono,Ryosaku

    1986-06-01

    Full Text Available The structure of hepatitis B virus surface antigen (HBs recognized by anti-HBs antibody was analyzed by western blotting using anti-HBs sera obtained from normal subjects, from rabbits immunized with purified HBs and commercially available goat serum. The HBs used had 7 components of 24 K, 27 K, 33 K, 36 K, 39 K, 43 K and 67-72 K daltons. Goat anti-HBs serum bound all of these components, while human and rabbit anti-HBs sera bound only two components (60 K and 54 K daltons, which were hardly visible in the gel even by silver staining. Mixing the 24 K and 27 K components, and the 24 K and 43 K components without reducing reagent produced several polymerized forms of HBs components including 60 K and 54 K polypeptides, which were recognized by anti-HBs rabbit serum. Other combinations of HBs components did not yield any new polymeric forms. Thus, it was concluded that the formation of anti-HBs antibody in normal subjects might predominantly require an antigenic structure of polymeric forms of specific combinations of HBs polypeptides, other than previously known antigenic determinants.

  3. Differential reactivity of mouse monoclonal anti-HBs antibodies with recombinant mutant HBs antigens

    Institute of Scientific and Technical Information of China (English)

    Azam Roohi; Yaghoub Yazdani; Jalal Khoshnoodi; Seyed Mohammad Jazayeri; William F Carman; Mahmood Chamankhah; Manley Rashedan; Fazel Shokri

    2006-01-01

    AIM: To investigate the reactivity of a panel of 8 mouse anti-hepatitis B surface antigen (HBsAg) monoclonal antibodies (mAbs) using a collection of 9 recombinant HBsAg mutants with a variety of amino acid substitutions mostly located within the "a" region.METHODS: The entire HBs genes previously cloned into a mammalian expression vector were transiently transfected into COS7 cells. Two standard unmutated sequences of the ayw and adw subtypes served as controls. Secreted mutant proteins were collected and measured by three commercial diagnostic immunoassays to assess transfection efficiency. Reactivity of anti-HBs mAbs with mutated HBsAgs was determined by sandwich enzyme-linked immunosorbent assay (ELISA).RESULTS: Reactivity of anti-HBs mAbs with mutated HBsAgs revealed different patterns. While three mutants reacted strongly with all mAbs, two mutants reacted weakly with only two mAbs and the remaining proteins displayed variable degrees of reactivity towards different mAbs. Accordingly, four groups of mAbs with different but overlapping reactivity patterns could be envisaged. One group consisting of two mAbs (37C5-S7 and 35C6-S11) was found to recognize stable linear epitopes conserved in all mutants. Mutations outside the "a"determinant at positions 120 (P→S), 123(T→N) and 161(M→T) were found to affect reactivity of these mAbs.CONCLUSION: Our findings could have important implications for biophysical studies, vaccination strategies and immunotherapy of hepatitis B virus (HBV) mutants.

  4. Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs

    Institute of Scientific and Technical Information of China (English)

    Hai-Ying Lu; Zheng Zeng; Xiao-Yuan Xu; Nai-Lin Zhang; Min Yu; Wei-Bo Gong

    2006-01-01

    AIM: To investigate the clinical significance and presence of mutations in the surface (S) and overlapping polymerase gene of hepatitis B patients with coexisting HBsAg and anti-HBs.METHODS: Twenty-three patients with chronic hepatitis B were studied. Of the 23 patients, 11 were both positive for hepatitis B virus (HBV) surface antigen (HBsAg) and antibody to HBV surface antigen (anti-HBs), 12 were negative for anti-HBs while positive for HBsAg. DNA was extracted from 200 μL serum of the patients. Nucleotide of the surface and overlapping polymerase gene from HBV-infected patients was amplified by PCR, and the PCR products were sequenced.RESULTS: Forty-one mutations were found within the surface gene protein of HBV in 15 patients (10 with coexisting HBsAg and anti-HBs). Six (14.6%) out of 41mutations were located at "α" determinant region in 5 patients (4 positive for HBsAg and anti-HBs). Eleven mutations (26.8%) occurred in the downstream or upstream of "α" determinant region. Lamivudine (LMV)-selected mutations were found in three patients who developed anti-HBs, which occurred in amino acid positions (196, 198, 199) of the surface protein and in YMDD motif (M204T/V) of the polymerase protein simultaneously. Presence of these mutations did not relate to changes in ALT and HBV DNA levels.CONCLUSION: Besides mutations in the "α" determinant region, mutations at downstream or upstream of the "α" determinant region may contribute to the development of anti-HBs. These mutations do not block the replicating competency of HBV in the presence of high titer of anti-HBs.

  5. Can HB vaccine yield a booster effect on individuals with positive serum anti-HBs and anti-HBc markers?

    Institute of Scientific and Technical Information of China (English)

    Ru-Xiang Wang; Christina Y. Shi; Ying Guo; Chang-Hong Yang; Yu Song; Juan Chen; Fu-Sheng Pang; Shao-Ping Lei; Xiao-Ming Jia; Jin-Ying Wen

    2004-01-01

    AIM: To evaluate if HB vaccination can yield a booster effecton the anti-HBs level of those naturally acquired HBV positivemarkers.METHODS: Sera were collected from 1399 newly enrolleduniversity students aged between 18-20 years at theentrance medical examination in 2001. Forty-four students(28 males and 16 females) with positive serum anti-HBs and anti-HBc markers served as an observation group and another 44 students (24 males and 20 females) without any HBV markers as the control. HB vaccination was given to all the students without positive serum HBs Ag according to 0,1, 6 month regimen and the peripheral venous blood was sampled from those of both observation and control groups for anti-HBs detection one month after the second and third doses. Anti-HBs levels were measured by ELISA.RESULTS: The seroconversion rate of anti-HBs in the control group was 100% after the second dose, but the geometric mean titers (GMTs) were low. The tendency of serum anti-HBs changes after the 3rd dose was completely different between the two groups. Although more than half of those with positive anti-HBs and anti-HBc showed a mild increase of anti-HBs levels after the 2nd boosting dose (mean anti-HBs level was 320:198 mIU), but the increase of serum anti-HBs titer was much smaller than that in the control group. The averages of their initial serum anti-HBs levelsand the levels after the 2nd and 3rd doses were 198, 320 and 275 mIU respectively. All the subjects from the control group had an obvious increase in their serum anti-HBs levels which was nearly 4 times the baseline level (302:78 mIU).CONCLUSION: HB vaccination can not enhance anti-HBs levels in those with positive serum anti-HBs and anti-HBc markers.

  6. Epidemiological characteristics of the carriers with coexistence of HBsAg and anti-HBs based on a community cohort study.

    Science.gov (United States)

    Pu, Z; Li, D; Wang, A; Su, H; Shao, Z; Zhang, J; Ji, Z; Gao, J; Choi, B C K; Yan, Y

    2016-04-01

    The coexistence of HBsAg and anti-HBs is an atypical serological pattern in HBV infection. There is no epidemiological characteristics of this serological pattern in the community and there is controversy over the molecular mechanisms underlying this pattern. We investigated the epidemiological characteristics of the carriers with HBsAg and anti-HBs in a longitudinal community cohort study. The prevalence of this atypical serological pattern was 2.93% (122/4169) in HBsAg-positive populations. The prevalence progressively increased with age from 40 to 70 years old. The rate of HBeAg positive and detectable HBV DNA were both significantly higher in carriers with this pattern than in carriers who were HBsAg positive but anti-HBs negative (26/122 verse 598/4047, P = 0.046; 86/122 verse 275/529,P < 0.001). After 1 year of follow-up, 85.19% of the carriers still had coexistence HBsAg and anti-HBs, 14.81% of the carriers lost their anti-HBs. Viral sequencing showed that carriers with coexistence of HBsAg and anti-HBs had higher numbers of residue changes within the S gene than carriers who were HBsAg positive but anti-HBs negative (2.42 verse 1.33 changes per 100 residues, P < 0.05). Hence, the coexistence of HBsAg and anti-HBs is a unique serological pattern which may be associated with an increased risk of adverse clinical outcome and may be related to HBsAg immune variants which have genotypic heterogeneity.

  7. Kan vericilerde HBsAg, anti-HCV, anti-HIV ve Sifilis seroprevalansı

    OpenAIRE

    Altındiş, Mustafa; Aslan, Savaş; Kalaycı, Raike

    2011-01-01

    Bu çalışmada Afyon Kocatepe Üniversitesi Tıp Fakültesi Kan Merkezi'ne başvuran donörlerde HBsAg, anti-HCV, anti-HIV 1/2 ve RPR seroprevalansının tespiti ve oranların yıllara ve cinsiyete göre dağılımının belirlenmesi amaçlanmıştır. Ocak'2001 ve Aralık'2010 tarihleri arasında Kan Merkezi'ne başvuran 37343 kan donörünün HBsAg, anti-HCV, anti-HIV ½ tarama testleri mikropartikül ELISA (Vitros, Ortho-Clinical Diagnostics) yöntemi ile Ortho-Clinical Diagnostics (HBsAg, HCV 3.jenerasyon, HIV 1/2) ki...

  8. Inmunogenicidad de una vacuna recombinante anti-HBs en trabajadores de la salud, del Instituto de Medicina Legal de Colombia

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    Heber Siachoque

    2007-12-01

    Full Text Available Objetivo: Evaluar la inmunogenicidad de la vacuna recombinante Hepavax-Gene para hepatitis B desde la última dosis administrada en trabajadores del INML.Materiales y métodos: Estudio de corte transversal en 603 trabajadores de la salud con mínimo 3 dosis de vacuna recombinante (0, 1, 6 meses donde se midieron los niveles de anticuerpos anti-HBs con la técnica de ELISA entre diciembre de 2000 y enero de 2001 desde la aplicación de la última dosis de la vacuna, que varió entre 1 y 6 años.Resultados: El grupo de estudio lo conformaron 344 hombres y 259 mujeres, con un promedio de edad de 38.8±7.3 años. El nivel de protección fue 90.7% (>10 U/l que disminuyó significativamente con el tiempo de aplicación de la última dosis de la vacuna (p45 (RRI=3.58, IC 95%:1.83, 6.99 con respecto a <40 años. Conclusión: La vacuna recombinante Hepavax-Gene anti-HBs tiene alta efectividad en los trabajadores de la salud (90.7% aunque presenta disminución de protección a mayor tiempo de aplicación de la última dosis y al aumentar la edad del trabajador.

  9. Seroprevalencia de anticuerpos anti-Toxoplasma gondii en pacientes con afecciones Oncohematológicas

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    Dailín Cobos Valdes

    2009-01-01

    Full Text Available Se analiza la reactividad de 23 muestras de suero de pacientes con hemopatías malignas provenientes de la sala de hematología del Hospital Clínico Quirúrgico de Holguín, mediante la técnica de Inmunofluorescencia Indirecta, reportándose un 65,2% de seroprevalencia. La Toxoplasmosis es una enfermedad causada por el agente etiológico Toxoplasma gondii, parásito intracelular obligado ampliamente distribuido en todo el mundo y capaz de infectar un gran número de huéspedes, incluida la especie humana. Numerosas han sido las investigaciones sobre la presencia de anticuerpos anti- Toxoplasma gondii en individuos inmunodeficientes, donde los pacientes con enfermedades oncohematológicas son un grupo representativo dentro de esta población.

  10. Seropositivity of HBsAg, anti-HCV and anti-HIV in preoperative patients

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    Berrin Karaayak Uzun

    2014-12-01

    Full Text Available Objective: The infections caused by human immunodeficiency virus (HIV, hepatitis B (HBV and C (HCV viruses pose a serious occupational risk for the healthcare workers especially those in emergency services, laboratories and surgery wards. Vaccination and establishment of the strict biosafety procedures are the main principles to prevent blood-borne infections in healthcare workers. Additionally, serological screening of the preoperative patients could decrease the risk for exposure. In this study, we aimed to determine the seroprevalence of HBsAg, anti-HCV, anti-HIV 1/2 in preoperative patients. Methods: Hospital automation records were evaluated retrospectively for 4.367 patients who were scheduled for surgery and scanned for anti-HIV 1/2, HBsAg and anti-HCV as preoperative procedures in the preparation period of operation between January 2012 and December 2012. Results: HBsAg positivity rate was found in 7.7% (n=336, anti-HCV positivity rate was found in 2.3% (n=101. A two (0.05% of five patients were positive for anti-HIV 1/2 was found positive verification test and the other three samples were accepted as false positive test results. Conclusion: All healthcare workers must be trained about occupational diseases and vaccinated against Hepatitis B. Universal precautions must be strictly followed particularly in the operating room. In addition, all patients should be considered as potential carriers regarded as a carrier of the potential for infection. J Clin Exp Invest 2013; 4 (4: 449-452

  11. Evaluation of Serum Anti-Hbs Concentration in Children Vaccinated with Recombinant Hepatitis B Vaccine at Birth

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    M Nejad-Ghaderi

    2006-07-01

    Full Text Available Introduction: Vaccination with the major surface antigen of hepatitis B virus (HBsAg induces anti-HBs antibody production and level of 10 IU/L is considered protective. It has been shown that the level of anti-HBs antibody does wane after vaccination. The aim of this study was to evaluate the persistence of anti-HBs antibodies in healthy Iranian children 10 years after primary vaccination. Methods: Blood samples were collected from 146 children, 10 years after completion of primary hepatitis B vaccination course at birth. The sera were tested for anti-HBs, antibody to hepatitis B core antigen (anti-HBc and HBsAg by use of ELISA technique. Results: At 10 years after primary vaccination, 70 (47.9% children had protective levels of antibody (anti-HBs> 10 IU/L with mean titer of 68.1 IU/ml. Moreover, 45 (30.82% children were negative for anti-HBs antibody. Distribution of children according to anti-HBs concentration revealed that the proportion of subjects with antibody titer of 0-10 IU/L, 10-100 IU/L, 100-500 IU/L and 500-1000 IU/L was 52.1%, 24.6%, 20.5% and 2.7%, respectively. All children were negative for HBsAg, although anti-HBc was positive in 11 (7.5% children. There was no difference in the seroprotection rates of males and females. Conclusion: The results of present study show that after 10 years after primary vaccination with recombinant HB vaccine, 47.9% of the children had protective levels of anti-HBs antibody. On basis of the HBsAg and anti-HBc results, it seems that effective immunological memory exists in children. Additional follow-up studies need to be conducted to determine the duration of protection.

  12. Evaluation of Serum Anti-Hbs Concentration in Children Vaccinated with Recombinant Hepatitis B Vaccine at Birth

    OpenAIRE

    M Nejad-Ghaderi; Mozafari, A.; J Montazerifar; GH Hassanshahi; HR Rashidi-Nejad; A Jafarzadeh

    2006-01-01

    Introduction: Vaccination with the major surface antigen of hepatitis B virus (HBsAg) induces anti-HBs antibody production and level of 10 IU/L is considered protective. It has been shown that the level of anti-HBs antibody does wane after vaccination. The aim of this study was to evaluate the persistence of anti-HBs antibodies in healthy Iranian children 10 years after primary vaccination. Methods: Blood samples were collected from 146 children, 10 years after completion of primary hepatitis...

  13. Mutations in the S gene and in the overlapping reverse transcriptase region in chronic hepatitis B Chinese patients with coexistence of HBsAg and anti-HBs

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    Feng Ding

    2016-02-01

    Full Text Available Abstract Background The mechanism underlying the coexistence of hepatitis B surface antigen and antibodies to HBsAg in chronic hepatitis B patients remains unknown. Aims This research aimed to determine the clinical and virological features of the rare pattern. Methods A total of 32 chronic hepatitis B patients infected by HBV genotype C were included: 15 carrying both HBsAg and anti-HBs (group I and 17 solely positive for HBsAg (group II. S gene and reverse transcriptase region sequences were amplified, sequenced and compared with the reference sequences. Results The amino acid variability within major hydrophilic region, especially the “a” determinant region, and within reverse transcriptase for regions overlapping the major hydrophilic region in group I is significantly higher than those in group II. Mutation sI126S/T within the “a” determinant was the most frequent change, and only patients from group I had the sQ129R, sG130N, sF134I, sG145R amino acid changes, which are known to alter immunogenicity. Conclusions In chronic patients, the concurrent HBsAg/anti-HBs serological profile is associated with an increased aa variability in several key areas of HBV genome. Additional research on these genetic mutants are needed to clarify their biological significance for viral persistence.

  14. Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix

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    Vázquez-Corzo Sara

    2003-01-01

    Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.

  15. Presence of maternal anti-HBs antibodies does not influence hepatitis B vaccine response in Brazilian neonates

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    Ana Luiza N Junqueira

    2011-02-01

    Full Text Available Recently, it was suggested that maternal hepatitis B surface antigen antibodies (anti-HBs acquired transplacentally could play a negative role in newborn infants' immune response to the hepatitis B vaccine. We compared the hepatitis B virus (HBV vaccine response in infants born to mothers previously vaccinated against HBV (n = 91 to infants born to mothers who were not previously vaccinated (n = 221. All newborn infants received three intramuscular doses (10 μg of HBV vaccine (Butang® at 0,1 and six months. The first dose was administered at the maternity hospital within 12 h of birth. The geometric mean titres of anti-HBs were not different among newborn infants born to mothers who were anti-HBs-negative (492.7 mIU/mL and anti-HBs-positive (578.7 mIU/mL (p = 0.38. Eight infants did not respond to the HBV vaccine. Of them, six were born to anti-HBs-negative mothers and two were born to mothers with anti-HBs titres less than 50 mlU/mL. Despite the mother's anti-HBs-positive status, our data show a good immunogenicity of the Brazilian HBV recombinant vaccine in neonates.

  16. Presence of maternal anti-HBs antibodies does not influence hepatitis B vaccine response in Brazilian neonates.

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    Junqueira, Ana Luiza N; Tavares, Viviane R; Martins, Regina M B; Frauzino, Kamilla V; Silva, Agabo M da Costa e; Rodrigues, Izolina M X; Minamisava, Ruth; Teles, Sheila A

    2011-02-01

    Recently, it was suggested that maternal hepatitis B surface antigen antibodies (anti-HBs) acquired transplacentally could play a negative role in newborn infants' immune response to the hepatitis B vaccine. We compared the hepatitis B virus (HBV) vaccine response in infants born to mothers previously vaccinated against HBV (n = 91) to infants born to mothers who were not previously vaccinated (n = 221). All newborn infants received three intramuscular doses (10 μg) of HBV vaccine (Butang®) at 0,1 and six months. The first dose was administered at the maternity hospital within 12 h of birth. The geometric mean titres of anti-HBs were not different among newborn infants born to mothers who were anti-HBs-negative (492.7 mIU/mL) and anti-HBs-positive (578.7 mIU/mL) (p = 0.38). Eight infants did not respond to the HBV vaccine. Of them, six were born to anti-HBs-negative mothers and two were born to mothers with anti-HBs titres less than 50 mlU/mL. Despite the mother's anti-HBs-positive status, our data show a good immunogenicity of the Brazilian HBV recombinant vaccine in neonates.

  17. Significance of tests for HBeAg and anti-HBe in HBsAg positive blood donors.

    OpenAIRE

    Dow, B C; Macvarish, I; Barr, A.; Crawford, R J; Mitchell, R.

    1980-01-01

    A sensitive radioimmunoassay method for HBeAg and anti-HBe allowed 98.4% of HBsAg positive blood donors to be classified. HBeAg was more prevalent in blood donors under 30 years of age and anti-HBe was more prevalent in those over 30 years. The mean HBsAg concentration was four times greater in donors with HBeAg than in those with anti-HBe. No significant associations were found with sex, blood groups, or HBsAg subtypes. It is likely that this test system will be extremely useful to blood tra...

  18. DETECCIÓN DE ANTICUERPOS ANTI-Leishmania (Trypanosomatidae EN POBLACIONES CANINAS DEL DEPARTAMENTO DE SUCRE, COLOMBIA

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    Luís Enrique Paternina Tuirán

    2015-11-01

    Full Text Available RESUMENEl objetivo de la investigación fue determinar la prevalencia de anticuerpos anti-Leishmania en perros del departamento de Sucre, Colombia. Se analizaron 122 perros del área rural de los municipios de Ovejas, Sampués y Sincelejo, mediante la técnica de inmunofluorescencia indirecta (IFI. Anticuerpos anti-Leishmania fueron detectados en el 69,6 % (IC95 % = 61,1 – 78,2 de la población canina estudiada. En el municipio de Sincelejo la seropositividad fue del 72 % (IC95 % = 61,1 – 82,8, en el municipio de Sampués del 68,4 % (IC95 % = 52,3 – 84,5 y en el municipio de Ovejas del 55,5 % (IC95 % = 21,2 – 86,3. Los animales seropositivos fueron principalmente caninos jóvenes y adultos. El 17,6 % de los perros seropositivos mostró signos clínicos compatibles con leishmaniasis canina, entre los cuales la onicogrifosis, alopecia y caquexia fueron los más comunes. La alta frecuencia de caninos con anticuerpos a Leishmania detectada en el presente estudio, pone de manifiesto la hiperendemicidad de la leishmaniasis canina en esta zona del país, así como el alto riesgo de brotes epidémicos de la enfermedad.ABSTRACTThe aim of this survey was to determine prevalence of antibodies against Leishmania in dogs from Sucre, Colombia. We analyzed 122 dogs in rural areas from municipalities of Sampués, Ovejas and Sincelejo, by indirect immunofluorescence (IFA. Anti-Leishmania antibodies were detected in 69.6 % (95 % CI = 61.1 to 78.2 of the selected canine population. In the municipality of Sincelejo, Sampues and Ovejas, the seropositivities were 72 % (95 % CI = 61.1 to 82.8, 68.4 % (95 % CI = 52.3 to 84.5 and 55.5 % (95 % CI = 21.2 to 86.3 respectively. Seropositive animals were mainly young and adult dogs. The 17.6 % of seropositive dogs showed clinical signs compatible with canine leishmaniasis; onychogryphosis, alopecia and cachexia were the most common ones. The detected high frequency of canines with antibodies against Leishmania in the

  19. Evaluation of HBsAg, anti-HCV, anti-HIV and VDRL test results in blood donors

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    Deveci, Özcan; Tekin, Alicem; Günbay, Seda Sibel; Kılıç, Dilek; KAYGUSUZ, Sedat; Ağalar, Canan; Özer, Türkan Toka

    2011-01-01

    Objectives: The most frequently encountered complication in the transfusion of blood and blood products are transmitted infections from these products. Infections caused by hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) remain the leading most important health problems in the transfusion of blood and blood products worldwide. Therefore, screening tests such as HBsAg, anti-HCV, anti-HIV, and RPR or VDRL for Treponema pallidum are mandatory tests to loo...

  20. [Requirement of standardizing anti-HBs assay methods in Japan for HBV infection-preventing strategy--discrepancy of anti-HBs measurements among three different kits widely used in Japan].

    Science.gov (United States)

    Ogata, Norio

    2006-09-01

    The strategy to eliminate hepatitis B virus (HBV) infection by administrating an HB vaccine is changing worldwide; however, this is not the case in Japan. An important concern about the HBV infection-preventing strategy in Japan may be that the assay methods for the antibody to hepatitis B surface antigen (anti-HBs) are not standardized. The minimum protective anti-HBs titer against HBV infection has been established as 10 mIU/ml by World Health Organization (WHO) -standardized assay methods worldwide, but that is still determined as a "positive" test result by the passive hemagglutination (PHA) method in Japan. We compared anti-HBs measurements in given samples among PHA(Mycell II, Institute of Immunology), chemiluminescent enzyme immunoassay (CLEIA) (Lumipulse, Fujirebio), and chemiluminescent immunoassay (CLIA) (Architect, Abbott), all of which are currently in wide use in Japan. First, anti-HBs measurements in serum from individuals who received a yeast-derived recombinant HB vaccine composed of the major surface protein of either subtype adr or subtype ayw were compared. The results clearly showed that in subtype adr-vaccinees CLIA underestimated the anti-HBs amount compared with CLEIA and PHA, but in ayw-vaccinees, the discordance in the measurements among the three kits was not prominent. Second, anti-HBs measurements in standard or calibration solutions of each assay kit were compared. Surprisingly, CLEIA showed higher measurements in all three kit-associated standard or calibration solutions than CLIA. Thus, the anti-HBs titer of 10 mIU/ml is difficult to introduce in Japan as the minimum protective level against HBV infection. Efforts to standardize anti-HBs assay methods are expected to share international evidence about the HBV infection-preventing strategy.

  1. Reactivation of viral replication in anti-HBe positive chronic HBsAg carriers

    DEFF Research Database (Denmark)

    Krogsgaard, K; Aldershvile, J; Kryger, Peter;

    1990-01-01

    Reactivation of hepatitis B virus replication was investigated in an unselected group of 44 HBV DNA negative, anti-HBe positive chronic HBsAg carriers. Twenty-five patients (54%) were intravenous drug addicts and 7 (16%) were male homosexuals. Sixteen patients had evidence of delta infection...... and five of the seven male homosexuals had human immunodeficiency virus infection. The patients were followed for 1 to 180 months (median, 24 months) while HBV DNA negative, anti-HBe positive. Reactivation, defined as reappearance of HBV DNA or HBeAg, or both, was detected in six patients corresponding...

  2. Reactivation of viral replication in anti-HBe positive chronic HBsAg carriers

    DEFF Research Database (Denmark)

    Krogsgaard, K; Aldershvile, J; Kryger, Peter;

    1990-01-01

    Reactivation of hepatitis B virus replication was investigated in an unselected group of 44 HBV DNA negative, anti-HBe positive chronic HBsAg carriers. Twenty-five patients (54%) were intravenous drug addicts and 7 (16%) were male homosexuals. Sixteen patients had evidence of delta infection and...... five of the seven male homosexuals had human immunodeficiency virus infection. The patients were followed for 1 to 180 months (median, 24 months) while HBV DNA negative, anti-HBe positive. Reactivation, defined as reappearance of HBV DNA or HBeAg, or both, was detected in six patients corresponding to...

  3. Anti-HBs in immunized children with cuban hepatitis B vaccine and impact of booster dose after five years

    International Nuclear Information System (INIS)

    Hepatitis B virus infection and associated diseases are a major public health problem. This study was planned to find out the persistence of antibody against hepatitis B surface antigen in Iranian vaccinated children after five years. Anti-HBs titers in a group of healthy good - responder children who were vaccinated with Cuban hepatitis B vaccine in infancy were measured after five years. Children with antibody titers <100 mlU/ml were revaccinated and retested after four weeks. Mean anti-HBs titers in 68 children (29 females, 39 males) were 482.1 mlU/ml at six months after the third dose of primary vaccination and 153 mlU/ml at five years later. Total mean anti-HBs titers in 36 (52.9%) children out of 68 (17 females, 19 males) were 38.3 mlU/ml and 4(5.8%) of 68 children (two of each sexes) had no detectable antibody after five years. Total mean anti-HBs titers in these hypo- responder and non- responder were 774.3 mlU/ml and 625.5 mlU/ml respectively after booster dose. In a group of children, who were immunized with Cuban hepatitis B vaccine from birth, anti-HBS titers fell at 6.5 years of age and almost half of children became hypo responder or no responder and their anti-HBs titers developed secondary rise after booster vaccination. All children showed immunologic memory to a booster dose. (author)

  4. Detection of HBsAg and Anti HBc on donors of a blood bank by IRMA and ELISA methods

    International Nuclear Information System (INIS)

    Comparative evaluation of two methods, Immunoradiometric Assay (IRMA) and Enzyme Immunoassay (ELISA), for detecting HBsAg and Anti HBc was made for determining which is the most advantageous and reliable. The study was made on 300 donors of the Hospital San Juan de Dios Blood Bank. In comparison with the reference method (IRMA), ELISA shows 91.67% of sensitivity. The Anti HBc detection by IRMA is more reliable than the HBsAg detection by IRMA and ELISA for determining the carrier state

  5. Screening of HBsAg and anti HCV from tertiary care, private and public sector hospitals

    International Nuclear Information System (INIS)

    Objectives: To find out the frequency of hepatitis B surface antigen and hepatitis C antibodies in patients referred from a tertiary care public sector hospital, other public sector and private hospitals of Karachi. Settings and duration: Pakistan Medical Research Council's Specialized Research Centre for Gastroenterology and Hepatology, at Jinnah Postgraduate Medical Centre Karachi from January to December 2009. Patients and Methods: A cross sectional study was conducted where patients were referred from different departments of Jinnah Postgraduate Medical Centre (tertiary care public sector hospital), other public sector hospitals, private hospitals and clinics for the screening of hepatitis B and C virus infection. Three ml blood was collected from each patient, serum separated and tested for HBsAg and Anti HCV using Abbott Murex fourth Generation ELISA kits. Results: A total of 2965 cases were referred in a year. Overall sero prevalence of HBsAg and Anti-HCV was 5.9% and 12.8% respectively. HBsAg positivity in patient referred from public sector hospitals was 5.8%, those from private hospitals/clinics were 7.2%, and self-referred patients was 5.6%. Anti HCV positivity rates amongst these cases were 12.5%, 16.7% and 8.5% respectively. Co-infection of hepatitis B virus and hepatitis C virus was seen in 0.9, 2.5 and 1.4% cases respectively. Breakdown of viral positivity within different departments of Jinnah Postgraduate Medical Centre Karachi showed HBsAg positivity of 7.1% in Medical department, 5.2% in Surgical department, 5.0% in Gynaecology department, 6.6% in other departments of Jinnah Postgraduate Medical Centre while, only 1.7% were positive from Pakistan Railway, hospital Anti HCV positivity was maximally (20.3%) seen in medical department followed by 14% in other departments, 10.9% in surgical department, 7.9% in gynaecology and 5.1% in railway hospital. Co-infection of HBV and HCV was seen in 2% cases referred from medical department, while rest of the

  6. Comparison of hepatitis B, core, HBc, and hepatitis B antibody, anti HBs, in a presumed low risk donor population.

    Science.gov (United States)

    Heck, Ellen; Cavanagh, H Dwight

    2014-09-01

    Donors screened by medical social history interview negative for high risk behavior or communicable disease history, but subsequently exhibiting reactive serological markers, emphasize importance of duel safe guarding factors for determining donor suitability. This report examines a relationship between two immunoabsorption assay tests, hepatitis B core (HBc) antibody, a required food and drug administration (FDA) test, and hepatitis B antibody (anti HBs), non-required test. Reactive serology results, 129 cases, 3,581 donors (2008-2012) for HBc as the only initially positive serological marker were subjected to anti HBs testing in this history pre-screened donor population. Enzyme linked immunoabsorption assay kits hepatitis B, core and antibody, were used in this study. All samples were initially tested for human immunodeficiency virus, hepatitis B, and hepatitis C, utilizing nucleic acid testing and antigen antibody immunoabsorption assay. Testing was performed by a FDA-registered CLEA-certified reference laboratory. Samples were deceased donor blood samples and a limited number of pre-mortem samples, separated, stored and analyzed according to manufacturer recommendation and FDA regulations. 129 reactive HBc only samples, were subsequently tested for anti HBs. Of these 129, 94 were found to be reactive for anti HBs. This represented 72 % of samples tested for antibody, a higher percentage than anticipated for a medical history negative, low risk population. PMID:24374389

  7. Vasculitis asociadas a anticuerpos anti-citoplasma de neutrófilos: Clínica y tratamiento

    Directory of Open Access Journals (Sweden)

    María Virginia Paolini

    2013-04-01

    Full Text Available Las vasculitis asociadas a anticuerpos anti-citoplasma de neutrófilos (ANCA comprenden a un grupo de enfermedades caracterizadas por la inflamación de la pared de pequeños vasos. Analizamos las características epidemiológicas y clínicas en una serie de 47 pacientes: 23 (49% granulomatosis de Wegener (GW, 15 (32% poliangeítis microscópica (PAM y nueve (19% vasculitis limitada al riñón (VLR. La edad media al inicio de los síntomas fue de 50.7 ± 14.9 años. La manifestación clínica más frecuente fue el compromiso renal en 41 (87% pacientes, seguido por el pulmonar en 26 (55% y el otorrinolaringológico en 17 (36%. En 26 (55% se asoció compromiso renal y pulmonar. La forma clínica más frecuente fue la generalizada en 23 (49%, seguida por la grave en 18 (38%. El 89% presentaron determinaciones de ANCA positivas. Cuatro (8% no recibieron tratamiento inmunosupresor de inicio. De los 43 que recibieron tratamiento de inicio, 29 (67% tuvieron remisión completa, con un tiempo de remisión promedio de 35.3 meses. Once (26% presentaron recaídas, diez (91% recaídas mayores y uno (9% menor. Doce (28% fallecieron, siete en forma temprana y cinco durante la evolución de la enfermedad. Quince (31% evolucionaron a insuficiencia renal crónica. Los 26 pacientes en seguimiento tuvieron respuesta al tratamiento y 20 (77% de ellos estaban en remisión al finalizar el estudio. Las vasculitis asociadas a ANCA continúan siendo enfermedades de alta morbilidad y mortalidad, a pesar de las mejorías logradas con los tratamientos inmunosupresores.

  8. Serum Anti-Hbs-Ag in Stable Hemodialysis Patients and its Relationship with Various Demographic and Biochemical Data

    Directory of Open Access Journals (Sweden)

    Azar BARADARAN

    2010-12-01

    Full Text Available Introduction: To evaluate the relationship between various biochemical, nutritional and demographic factors with immune response to hepatitis B vaccine in maintenance hemodialysis (HD patients. Material and Methods: A retro-prospective study was carried out on 68 patients undergoing maintenance hemodialysis .Patients were vaccinated against hepatitis B virus with an intramuscular hepatitis B vaccination schedule, 40 micrograms at 0, 1, and 6 months. We also selected 32 age matched normal healthy persons who had vaccinated against hepatitis B previously to compare the antibody production with HD patients. Results: The value of serum antibody level against hepatitis B surface antigen ( HBs in hemodialisis patients and healthy persons were 35±55(median=5.5 and 135±71 (median=175 mIU/ml respectively. There was a significant deference between mean serum antibody level against HBs antigen of hemodialysis patients and normal subjects (p<0.001, there were not any significant differences of antibody production against HBs antigen between males and females or diabetic and non diabetics. There were no correlation between serum antibody level against HBs-Ag and serum albumin and also with body mass index. There were not significant correlation between anti-HBs antibody level and age, amounts of hemodialysis, duration of dialysis, dialysis adequacy, serum ferritin level and serum lipids. There were not also significant correlation between anti-HBs antibody level and serum parathormone, calcium, phosphorus, serum hemoglobin and hematocrit level. Conclusion: In this study, there was not significant correlation between serum antibody level against hepatitis B surface antigen and various nutritional and demographic factors of patients under regular hemodialysis.

  9. Anticuerpos anti 21 hidroxilasa séricos en pacientes con anticuerpos antifracción microsomal: Síndrome poliendocrino autoinmune Seric 21- hydroxilase antibodies in patients with anti-microsomal fraction antibodies: Autoimmune polyendocrine syndrome

    Directory of Open Access Journals (Sweden)

    Silvia Botta

    2007-04-01

    Full Text Available El síndrome poliendocrino autoinmune (SPA es la asociación de enfermedades endocrinas autoinmunes con otros desórdenes autoinmunes no endocrinos. Los tipos 1, 2 y 4 presentan adrenalitis autoinmune, esto indica la presencia de autoanticuerpos, y su marcador serológico específico es el anti 21 hidroxilasa (a21-OH. El SPA tipo 2 es la asociación de adrenalitis, enfermedad tiroidea y/o diabetes mellitus inducidas por autoanticuerpos. Como componentes menores, pueden estar asociados entre otros, vitiligo, alopecia y miastenia. Nuestros objetivos fueron: establecer la prevalencia de a21-OH séricos en pacientes con anticuerpos anti fracción microsomal (AFM positivos, enfermedad tiroidea autoinmune y/o afecciones endocrinas y no endocrinas autoinmunes; diagnosticar formas incompletas de SPA y estudiar individuos con probable riesgo de progresión a un SPA completo. Estudiamos 72 pacientes AFM positivos y 60 sujetos tomados como grupo control, AFM negativos. Hallamos a21-OH elevados en dos pacientes: A= 47 U/ml, hipotiroidismo autoinmune y miastenia; y B= 8.75 U/ml, hipotiroidismo autoinmune y vitiligo; ambos con ausencia de insuficiencia adrenal. La prevalencia de a21-OH encontrada fue del 2.8%. Las pacientes A y B corresponden a un SPA tipo 2 incompleto y latente en relación al componente adrenal. Considerando a los a21-OH marcadores de enfermedad autoinmune latente, el eventual riesgo de evolución hacia la afección clínica sugiere la necesidad de estrechos controles clínicos y bioquímicos periódicos.Autoimmune polyendocrine syndrome (APS is the association of autoimmune endocrine diseases, with other autoimmune nonendocrine disorders. APS types 1, 2 and 4 include autoimmune adrenalitis; this suggests the presence of autoantibodies. A specific serological marker for these is the anti 21- hydroxilase autoantibody (a21-OH. APS type 2 is the association of autoimmune adrenalitis, to autoimmune thyroid disease and/or diabetes mellitus, all

  10. Primary research of hepatitis B virus infected patients serum with HBsAg and anti-HBs double positive%乙型肝炎病毒表面抗原与表面抗体同时阳性的血清学模式初步研究

    Institute of Scientific and Technical Information of China (English)

    王珍光; 邓芝云; 郭建巍; 马骢; 荣扬; 刘敏

    2011-01-01

    目的 分析乙型肝炎病毒感染者乙型肝炎表面抗原与乙型肝炎表面抗体同时阳性的血清学模式与HBV DNA 的关系,并探究其原因及临床意义.方法 用酶联免疫分析法筛选出HBsAg和抗-HBs同时阳性的标本,用化学发光微粒子免疫分析法确认,用实时荧光定量聚合酶链反应检测其HBV DNA含量.结果 在选取的HBsAg和抗-HBs双阳性的56人中,共出现4 种HBV血清学模式:(1)HBsAg、抗-HBs、抗-Hbe、抗-HBc 阳性模式占42.8%,HBV DNA阳性率41.6%;(2)HBsAg、抗-HBs、HBeAg、抗-HBc阳性模式占30.4%,HBV DNA阳性率70.5%;(3)HBsAg、抗-HBs、HBcAb阳性模式占25%,HBV DNA 阳性率21.4%.(4)HBsAg、抗-HBs、HBeAg 、抗-Hbe、抗-HBc阳性模式占1.8%,HBV DNA阳性率100%.56 例样本中,有27例血清HBV DNA 检测阳性,阳性率为48.2%.结论 HBsAg和抗-HBs 双阳性伴HBeAg阳性者,血清中有较高水平的HBV DNA.HBsAg 和抗-HBs 双阳性并不代表疾病好转.%Objective To analyze of serum with HBsA g and anti HBs double positive in patients with Hepatitis B virus infec tion and discuss the relationship with HBV DNA. Methods to screening HBsAg and anti HBs double positive serum with enzyme linked immunosorbent assay and check the serum HBV markers and HBV DNA by Immunochemiluminescent technology and real time PCR respectively. Results Among the 56 cases with HBsAg and anti-HBs double positive, there were four HBV serology models: (1)HBsAg,anti-HBs, HBeAg and anti-HBc positive, with a positive rate of 42.8 % and HBV DNA positive rate 41.6 %.(2)HBsAg,anti-HBs and anti-HBc positive with a positive rate 30.4% and HBV DNA positive rate 70.5%. (3)HBsAg,anti HBs,anti HBe and anti HBc positive with a positive rate 25% and HBV DNA positive rate 21.4%. (4)HBsAg,anti HBs,HBeAg,anti HBe and anti HBc positive model with a 1.8% positive rate and whole positive of HBV DNA. There were 27 cases in 56 HBsAg and anti HBs double positive samples with a positive rate of 48

  11. Presence of maternal anti-HBs antibodies does not influence hepatitis B vaccine response in Brazilian neonates

    OpenAIRE

    Ana Luiza N Junqueira; Viviane R Tavares; Regina MB Martins; Kamilla V Frauzino; Agabo M da Costa e Silva; Izolina MX Rodrigues; Ruth Minamisava; Teles, Sheila A

    2011-01-01

    Recently, it was suggested that maternal hepatitis B surface antigen antibodies (anti-HBs) acquired transplacentally could play a negative role in newborn infants' immune response to the hepatitis B vaccine. We compared the hepatitis B virus (HBV) vaccine response in infants born to mothers previously vaccinated against HBV (n = 91) to infants born to mothers who were not previously vaccinated (n = 221). All newborn infants received three intramuscular doses (10 μg) of HBV vaccine (Butang®) a...

  12. Prevention of de novo HBV infection by the presence of antiHBs in transplanted patients receiving core antibody-positive livers

    Institute of Scientific and Technical Information of China (English)

    Rafael Barcena; Gloria Moraleda; Javier Moreno; M Dolores Martín; Emilio de Vicente; Jesús Nu(n)o; M Luisa Mateos; Santos del Campo

    2006-01-01

    AIM: To analyze whether the presence of anti-HBs in liver transplant recipients is effective in preventing HBV infection.METHODS: Twenty-three patients receiving anti-HBc positive liver were studied. Nine recipients were anti HBc positive as a result of previous HBV infection. Of them, one also received HBV vaccine during the pre-liver transplantation period. Fourteen recipients were anti-HBs positive due to HBV vaccine administered during the pretransplant period. Liver biopsy was obtained in 10/14anti-HBc negative/anti-HBs positive recipients and in 4/9anti-HBc positive recipients.RESULTS: After a mean follow-up period of 46 months,1 recipient with protective serum anti-HBs levels developed de novo HBV infection as a consequence of immune escape HBV mutants. Among the 14 vaccinated anti-HBc negative/anti-HBs positive recipients, 1/10patients with available liver biopsy (10%) had liver HBVDNA at 13 mo post-liver transplantation without serum viral markers and did not develop de novo HBV infection.The vaccinated anti-HBc positive recipient without HBV vaccine response was HBV-DNA positive in serum and liver, viral DNA was continuously negative in the followlng tests, so a spontaneous seroconversion was diagnosed.CONCLUSION: The presence of anti-HBs as a result of HBV vaccine or past HBV infection seems to be effective at protecting patients receiving livers from anti-HBc positive donors. However, the emergence of immune escape HBV mutants, which can evade the anti-HBs protection, should be considered as a risk of HBV infection.

  13. Awareness and Practice of Complete Hepatitis B Vaccination and Anti-HBs Testing in Vaccinated Health Care Workers

    Directory of Open Access Journals (Sweden)

    Annapurna G. Sajjan

    2015-04-01

    Full Text Available Background: Hepatitis B is a serious and common infectious disease affecting millions of people worldwide. Health Care Workers (HCW are at an increased risk of occupational exposure to HBV and the incidence is 2-4 times higher than in the general population. Despite potential risks, awareness and vaccine compliance is poor among the HCWs. Aim: To assess the awareness of complete Hepatitis B vaccination, anti-HBs testing & protective titres and determine the anti HBs titres amongst vaccinated HCWs. Material & Methods: A total of 500 Health care workers of both sexes in the age group from 20- 60 years vaccinated against Hepatitis B were tested for anti-HBs titres by quantitative ELISA. Results: The rate of complete immunization was 81.4% in doctors, 63.3% in nursing staff and 90% in the technical staff. Amongst the 500 participants, 70.8% had received all the doses and 29.2% incomplete doses of the vaccine. Titres of ≥ 10 mIU/ml were demonstrated in 84.4% of HCWs who received all the doses and in 65.7% those who defaulted. Conclusions: The results of the study indicate lack of awareness about complete HB vaccination and the importance of post vaccination testing in HCWs.

  14. HBs Fab-IFNα融合蛋白的制备与初步鉴定%Preparation and preliminary identification of anti HBs Fab-IFNa fusion protein

    Institute of Scientific and Technical Information of China (English)

    陆慧琦; 宋杰; 叶伟民; 韩焕兴

    2011-01-01

    目的 构建抗HBsAg的pComb Fab-IFNα载体,原核表达具双重生物活性的抗HBs Fab-IFNα融合蛋白.方法 以pBAD-Fab和pBAD IFNα作为模板,分别扩增Fd、λ和IENα基因,经相应的限制性内切酶酶切后分3次克隆人pComBHss质粒,转化XL-1 Blue大肠埃希菌.限制性酶切和测序鉴定重组质粒,免疫蛋向印迹(Western blotting)和斑点印迹(Dot blotting)鉴定融合蛋白的表达及抗原结合活性.结果 重组载体的酶切、电泳及测序表明抗HBs Fab-IFNα基因克隆正确.表达产物经12%SDS-PAGE电泳、转印,Western blotting显示该融合蛋白分子量约为65kD,Dot blotting显示其与HBsAg具有结合能力.细胞病变抑制法测定IFNα生物学活性为7.8×104~5.1×105U/ml.结论 该原核系统成功表达了抗HBs Fab-IFNα融合蛋白,表明其既具有抗HBsAg结合能力,又具备IFNα的生物活性,为进一步的系统表达和应用研究提供了条件.%Objective To construct the anti HBsAg pComb Fab-IFNe vector, and to express the fusion protein consisting of IFN and anti-HBs Fab in prokaryon. Methods Using pBAD-IFN plasmid and pBAD-Fab plasmid as template, the anti-HBs Fd, and IFN were amplified separately with corresponding endonuclease sites by polymerase chain reaction (PCR). Each PCR product was digested with specific endonuclease and inserted into pComBHss vector, and then transformed into XL-1 Blue. The recombinant plasmid was isolated by miniprep for restriction analysis and sequencing. Fusion protein was identified by Western blotting and Dot blotting. Results The recombinant plasmid was confirmed by restriction electrophoresis and sequencing. Aliquots of human anti-HBs Fab-IFNα were concentrated and size fractionated by 12% SDS-PAGE and stained with Coomassie. The proteins were transferred to nitrocellulose, incubated with HRP- conjugated goat anti-human IgG Fab and Rah anti-human IFN. The molecular weight of the fusion protein was about 65kD. Anti HBs Fab-IFNa fusion

  15. Desarrollo de superficies modificadas oro - anticuerpo anti-PSPB para su uso como biosensor en la detección de preñez bovina

    OpenAIRE

    Justo Laíz; Karen Keel; Juan Pablo Tosar

    2011-01-01

    En este trabajo se presentan los resultados preliminares para el desarrollo de un biosensor amperométrico destinado a la cuantificación de Proteína B Específica de la Preñez (PSPB) en fluidos biológicos como medio de diagnóstico clínico precoz de la preñez bovina. A la superficie de oro modificada con ácido mercaptoundecanoico (MUA) se fijaron anticuerpos anti-PSPB y peroxidasa de rábano (HRP). Se verificó la inmovilización de las biomoléculas mediante voltamperometría cíclica y medida del án...

  16. Soroprevalência e fatores de risco para a infecção pelo vírus da hepatite B pelos marcadores AgHBs e anti-HBs em prisioneiros e primodoadores de sangue Seroprevalence and risk factors for hepatitis B by AgHBs and anti-HBs markers in prisoners and first-time donors, in Central Brazil

    Directory of Open Access Journals (Sweden)

    Celina Maria Turchi Martelli

    1990-08-01

    Full Text Available Foram rastreados 1.033 primodoadores de sangue e 201 prisioneiros, para a infecção pelo vírus da hepatite B (VHB, durante o período de junho de 1988 a fevereiro de 1989, em Goiânia, GO (Brasil. Foi estimada a soroprevalência dos marcadores AgHBs e anti-HBs, e estudados os fatores de risco associados a soropositividade. Os testes sorológicos foram realizados pela técnica de ELISA e a presença de qualquer dos marcadores estudados foi interpretada como exposição ao vírus da hepatite B. Um questionário padronizado foi aplicado em ambos os grupos populacionais para avaliar: transfusão sangüínea prévia, número de parceiros sexuais, atividade homo/bissexual, história de outras doenças sexualmente transmissíveis, uso de droga injetável, uso de medicação injetável, acupuntura, tatuagem e soropositividade ao VDRL. Foram obtidas soroprevalências globais para a infecção pelo VHB de 26,4% e 12,8% para a população carcerária e de primodoadores respectivamente, diferença estatisticamente significante (pTwo cross-sectional surveys on hepatitis B virus (HBV infection were carried out among 1,033 volunteer first-time blood donors in five blood banks (3 private, 2 public and among 201 prisoners in the Penitentiary Center of Industrial Activity, in Goiânia, Central Brazil, between June 1988 and February 1989. Those surveys were part of a major study designed to estimate seroprevalence of HBsAg and anti-HBsAg markers by ELISA test, and to study risk factors associated with seropositivity. The presence of any serum marker was considered as previous exposure to HBV. A standard questionnaire was applied to both populations to evaluate previous blood transfusion, number of sexual partners, homo/bisexual activity, history of sexually transmitted diseases, drug abusers, use of parenteral medicine, accupunture, tattooing and VDRL seropositivity. Seroprevalence varied from 12.8% to 26.4% in blood donors and prisoners, respectively, (p<0

  17. Determinación de anticuerpos anti-β2glicoproteína I en pacientes con síndrome antifosfolípido Anti- β2 glycoprotein antibodies in patients with antiphospholipid syndrome

    Directory of Open Access Journals (Sweden)

    Oscar Uribe Uribe

    2004-09-01

    Full Text Available EL objetivo de este estudio fue comparar la presencia de anticuerpos anti-β2glicoproteína I (anti- β2GPI con las pruebas convencionales de laboratorio de anticuerpos anticardiolipina (aCL y anticoagulante lúpico, y con las manifestaciones clínicas del síndrome antifosfolípido (SAF. Se incluyeron en el estudio 80 mujeres con SAF; 35 de ellas de la consulta de Reumatología y las otras 45 con historia de aborto recurrente espontáneo (ARE; 5 mujeres de la consulta de Reumatología sin SAF, 27 mujeres con ARE, sin SAF y un grupo control de 20 mujeres sanas en edad reproductiva. Se investigaron la presencia de anticuerpos IgG e IgM anticardiolipina (aCL e IgG anti- β2GPI por la técnica de ELISA, y el anticoagulante lúpico por la determinación del tiempo parcial de tromboplastina activado. Adicionalmente, se registraron las manifestaciones clínicas asociadas al SAF. De las pacientes con SAF, 25.7% del grupo de Reumatología (9/35 y 4.4% de las pacientes con ARE (2/45 fueron positivas para anticuerpos anti-β2GPI, mientras que ninguna de las mujeres sin SAF, ni de las mujeres del grupo control, fue positiva. La asociación entre la presencia de anti- β2GPI y los anticuerpos IgG e IgM aCL mostró una diferencia significativa en los títulos de 3+ (altamente positivos en contraste con los individuos negativos para anti- β2GPI. La positividad del anticoagulante lúpico también se correlacionó con la presencia de anticuerpos anti- β2GPI. No hubo diferencia significativa entre las diversas manifestaciones clínicas del SAF y la presencia de dichos anticuerpos. En conclusión, la determinación de anticuerpos anti- β2GPI tiene una alta especificidad en pacientes con SAF pero no se asoció con ninguna manifestación clínica en particular. The objective of this study was to compare the presence of anti-β2glycoprotein (anti- β2GPI antibodies with the conventional laboratory tests of anticardiolipin antibodies (aCL and lupus anticoagulant

  18. "PERSISTENCE OF ANTI-HBs ANTIBODIES IN HEALTHY IRANIAN CHILDREN VACCINATED WITH RECOMBINANT HEPATITIS B VACCINE AND RESPONSE TO A BOOSTER DOSE"

    Directory of Open Access Journals (Sweden)

    A. Jafarzadeh

    2005-05-01

    Full Text Available Long-term protection against hepatitis B virus (HBV is dependent on persistence of anti-HBs antibodies and/or strong immunological memory. In this study we evaluated the persistence of anti-HBs antibodies in healthy Iranian children 5 years after primary vaccination and the response to a booster dose using recombinant hepatitis B vaccine. Totally, 81 children who had received primary course of hepatitis B vaccine at 0, 1.5 and 9 months of age were included in this study. A booster dose of hepatitis B vaccine was administered at 5 years after completion of primary vaccination program. Children were tested for anti-HBs antibody just before administration of booster dose and at 4 weeks after booster vaccination. An 81.5% seroprotection rate (anti-HBs > 10 IU/L was observed 5 years after primary vaccination. After administration of booster dose, 100% of the children developed protective level of anti-HBs antibody and geometric mean titer rose from 206 IU/L to 1278 IU/L. These results indicate the existence of an effective immunological memory over a period of 5 years after primary vaccination with recombinant hepatitis B vaccine in healthy Iranian children.

  19. Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix Association between anti-Ras and anti-HPV16 E4/E7 antibodies with cervical intraepithelial lesions

    Directory of Open Access Journals (Sweden)

    Sara Vázquez-Corzo

    2003-10-01

    Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.OBJECTIVE: To evaluate whether serum antibodies anti-E4, E7 and Ras could be used as markers for early cervical lesions associated with HPV (human papillomavirus. MATERIAL AND METHODS: A seroepidemiological case-control study was conducted between March 1999 and April 2000 at the dysplasia clinic of Hospital General Doctor Gea Gonzalez, in Mexico City, to evaluate the presence of antibodies anti-E4, E7, and Ras through a sandwich ELISA. Analysis was done using odds ratios and 95% confidence intervals. RESULTS: Anti-E7 antibodies were associated to women with CIN III lesions, while anti-E4 and Ras antibodies were strongly associated with CIN I-II lesions. The antibody profile of women with different

  20. Hepatitis B virus screening in contacts of blood donors with antibodies against core protein (anti-HBc, but without surface antigen (HBsAg

    Directory of Open Access Journals (Sweden)

    Hildenete Monteiro Fortes

    2006-03-01

    Full Text Available To increase blood safety Brazil introduced screening for anti-HBc among blood donors in 1993. There was a decrease in the hepatitis B virus (HBV transmission, but this measure identified a great number of HBsAg-negative, anti-HBc-positive donors. Surveillance policy determines that contacts of HBV carriers should be screened to HBV markers, but there is no recommendation about how to guide contacts of HBsAg-negative, anti-HBc-positive donors. Aiming to evaluate whether the contacts of this group are at greater risk for HBV infection, a cross-sectional study was performed to compare prevalence of HBV infection between contacts of HBsAg-positive blood donors (group I and contacts of HBsAg-negative, anti-HBc-positive donors (group II. Contacts were submitted to a questionnaire and blood tests for HBV markers. In group I (n = 143, 53 (37.1% were anti-HBc-positive and 11 (7.7% were HBsAg-positive. In group II (n = 111, there were 9 and 0.9%, respectively. HBV exposure was associated with group I, sexual activity, blood transfusion, being one of the donor's parents, and living for more than ten years with the donor. Regarding the families as sample units, it was more common to find at least one member with HBV markers (p < 0.05 among the families of group I compared to group II. Contacts of HBsAg-negative, anti-HBc-positive individuals presented a much lower risk of having already been exposed to HBV and there is no need to screen them for HBV in low to moderate prevalence populations.

  1. "PERSISTENCE OF ANTI-HBs ANTIBODIES IN HEALTHY IRANIAN CHILDREN VACCINATED WITH RECOMBINANT HEPATITIS B VACCINE AND RESPONSE TO A BOOSTER DOSE"

    OpenAIRE

    Jafarzadeh, A; S. M. A. Sajjadi

    2005-01-01

    Long-term protection against hepatitis B virus (HBV) is dependent on persistence of anti-HBs antibodies and/or strong immunological memory. In this study we evaluated the persistence of anti-HBs antibodies in healthy Iranian children 5 years after primary vaccination and the response to a booster dose using recombinant hepatitis B vaccine. Totally, 81 children who had received primary course of hepatitis B vaccine at 0, 1.5 and 9 months of age were included in this study. A booster dose of he...

  2. Patrón de immunoblotting y niveles de anticuerpos anti-Toxoplasma gondii en suero y humor acuoso de pacientes con lesiones de toxoplasmosis ocular

    Directory of Open Access Journals (Sweden)

    Morella Bouchard Pereira

    2014-07-01

    Full Text Available El objetivo de este estudio fue evaluar en muestras de suero y humor acuoso los niveles de anticuerpos anti-toxoplasma a través del Coeficiente de Goldmann y Witmer (CGW y el patrón de reconocimiento antigénico a través del immunoblotting (IB, en pacientes con serología positiva con y sin lesiones de toxoplasmosis ocular. Se recogieron simultáneamente muestras de suero y humor acuoso de 26 pacientes: un grupo de casos que poseían lesiones retinales de toxoplasmosis ocular en fase activa e inactiva (n=17 y un grupo control que requería cirugía ocular por presencia de cataratas (n=9. Las determinación de IgM e IgG específicas se realizó por ELISA de inmunocaptura e indirecto, respectivamente. Se utilizó la inmunodifusión radial para la cuantificación de la IgG total. El CGW resultó >2, indicativo de producción local de anticuerpos específicos en 12/17 de los casos, mientras que en los controles no se observó, esto evidenció una sensibilidad del 71% y una especificidad de 100%. En IB, la aparición de bandas diferentes en humor acuoso, indicativo de producción local de anticuerpos específicos se observaron en 11/17 de los casos y 1/9 de los controles, reflejando una sensibilidad de 65% y una especificidad de 89%. Al considerar las dos pruebas la sensibilidad se incrementó a un 73%, pero la especificidad disminuyó a 89%. En conclusión el IB es útil como prueba confirmatoria para diagnóstico de toxoplasmosis ocular, pero sólo como un complemento del coeficiente de GW especialmente en pacientes con lesiones atípicas donde el diagnóstico clínico es difícil. Aqueous humor and serum immunoblotting profiles and anti–toxoplasma gondii antibodies in patients with toxoplasmosis-induced retinal lesions Abstract The purpose of this study was to analize the anti-Toxoplasma gondii antibodies levels in serum and aqueous humor samples in patients with ocular toxoplasmosis by using Goldman and Witmer Coefficient (GWC and the

  3. Detección de anticuerpos Anti-brucella en focas de Weddell (Leptonychotes weddellii de Cabo Shirref, Antártica Detection of anti-brucella antibodies in Weddell seals (Leptonychotes weddellii from cape Shirref, Antarctica

    Directory of Open Access Journals (Sweden)

    O. BLANK

    2002-01-01

    Full Text Available Posterior al hallazgo de anticuerpos anti-Brucella en muestras de lobo fino antártico (Arctocephalus gazella, el estudio serológico en Pinnipedia Antárticos se continuó con el fin de determinar la presencia de anticuerpos anti-Brucella en otras especies. Se colectaron muestras de sangre y de fluido extravascular de 12 ejemplares de foca de Weddell (Leptonychotes weddellii encontrados en el Sitio de Especial Interés Científico (SEIC Nº 32 y sitio CCAMLR Ecosystem Monitoring Program (CEMP N° 2, "Cabo Shirreff e Islotes San Telmo" (62º 47' S; 60º 27' W, localizado en la costa Noroeste de la isla Livingston (Shetland del Sur, Antártica. Las muestras obtenidas fueron analizadas por la técnica convencional Rosa de Bengala (RB y dos inmunoensayos enzimáticos de competencia: Compelisa®, y c-ELISA. De las muestras estudiadas se identificaron cinco con anticuerpos anti-Brucella, siendo las pruebas inmunoenzimáticas las técnicas más sensibles. Estos resultados muestran una alta probabilidad de presencia de infección por una bacteria del género Brucella en ejemplares L. weddellii y plantean la necesidad de realizar estudios complementarios, que permitan conocer la etiología y entender la epidemiología de Brucella en esta región del mundoAfter the finding of anti-Brucella antibodies in samples of Antarctic fur seal (Arctocephalus gazella, the serological study on Antarctic Pinniped was continued in order to determine the presence of anti-Brucella antibodies in other species. Blood and extra vascular fluid samples were taken from 12 Weddell seals (Leptonychotes weddellii at the Site of Special Scientific Interest (SSSI Nº 32 and CCAMLR* Ecosystem Monitoring Program (CEMP site N° 2 "Cape Shirreff and San Telmo Islets" (62º 47' S; 60º 27' W, located on the Norwest coast Livingston Island (South Shetland Islands, Antarctica. The samples were tested by the conventional Rose Bengal test (RB and two competitive enzymatic immunoassay

  4. Purification and Analysis of Structure of Fab Fragment of Recombinant Human Anti-HBs%重组人抗HBs-Fab抗体的纯化及结构分析

    Institute of Scientific and Technical Information of China (English)

    饶桂荣; 陈文吟; 粟宽源; 任向荣; 郑业华; 余宙耀

    2006-01-01

    目的研究重组人抗HBs-Fab抗体的纯化工艺,并对其结构等特性进行分析.方法采用离子交换-分子筛层析法分离纯化由酵母工程菌(GS115/Fab)发酵的重组人抗HBsAg Fab抗体,并经ELISA检测其抗体活性,等电聚焦电泳法检测其等电点(pI),基质辅助激光解吸飞行时间质谱(MALDI-TOF-MS)检测其相对分子质量和肽质量图谱.结果纯化的重组Fab抗体的纯度可达90%以上,经Sephacryl-100进一步层析后,纯度达99%以上,总收率可达80%以上.Fab抗体具有较好的抗体活性,其pI值为7.6,为一碱性蛋白,相对分子质量为50 494,比其理论值约多2 579.35,经Endoglycosidase H内切酶消化后的相对分子质量为49 609,证明Fab的一级结构中有糖基化现象,且分布于Fab抗体的H链和L链上.胰酶酶解肽段中有21个与理论肽段相符,另检测到1对二硫键正确.结论已建立了重组人抗HBs-Fab抗体的稳定的纯化工艺,且Fab抗体的一级结构正确.

  5. Neuromielitis óptica con alta expresión de acuaporina-4 y anticuerpos anti-acuaporina-4 positivos en suero

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    Alejandra Báez

    2012-04-01

    Full Text Available La presencia de anticuerpos IgG en suero, con blanco en los canales de acuaporina-4, es específica de la neuromielitis óptica (NMO. El 60% de los pacientes con NMO presentan lesiones cerebrales en la resonancia magnética (RM; en un 8% (mayoría niños estas lesiones se consideraron "atípicas". Presentamos dos pacientes con NMO y lesiones en el SNC de alta expresión de acuaporina-4. Caso 1: varón de 50 años, que comenzó con pérdida de visión en ojo derecho (OD. Recibió tratamiento empírico con metilprednisolona 1 g/d x 3 días. Al mes presentó dolor generalizado y hemiparesia derecha; nuevamente recibió metilprednisolona 1 g/d x 5 días e IgG IV 400 mg/kg/d × 5 días. Recuperó la deambulación persistiendo el dolor y fenómenos paroxísticos en los 4 miembros. Potenciales evocados visuales: P100, ojo izquierdo (OI 123 mseg. OD sin respuesta. La RM de cerebro (FLAIR mostró hiperintensidad en nervio óptico derecho, hipotálamo y comisura blanca anterior. RM cervical: lesión medular extensa (5 cuerpos vertebrales. Caso 2: mujer de 53 años, con disminución de la agudeza visual en ambos ojos y parestesias en miembros inferiores que remitieron espontáneamente. Evolucionó al mes con cuadriparesia e incontinencia esfinteriana. Recibió metilprednisolona 1 g/d x 5 días, sin mejoría. Potenciales evocados visuales: P100 OI 124 mseg. OD 128 mseg. RM cerebro: (FLAIR hiperintensidad hipotalámica y periacueductal. RM cervical: lesión medular extensa (7 cuerpos vertebrales. Anticuerpos anti-acuaporina-4 positivos en ambos pacientes (inmunofluorescencia indirecta. Las lesiones consideradas "atípicas", como aquí, en sitios con alta densidad de proteínas canales de agua AQP4 deberán considerarse para el diagnóstico diferencial.

  6. Determinación de las condiciones de ensayo óptimas en un ELISA para la detección de anticuerpos séricos IgG anti-LPS de Pseudomonas aeruginosa O11

    OpenAIRE

    Tania Valmaseda; Lázaro Alba; Rolando Ochoa; Aniel Moya; Yadira Pino; Sara Catalina Esnard

    2004-01-01

    La selección de sueros de donantes sanos para la obtención de una gamma hiperinmune contra la infección por Pseudomona aeruginosa, así como la evaluación de la respuesta inmunológica de cualquier candidato vacunal contra este microorganismo necesita contar en el laboratorio con técnicas estandarizadas. En este trabajo se realizaron los ensayos necesarios para el montaje y la optimización de un ELISA indirecto para la determinación de anticuerpos séricos de clase IgG anti- LPS de P. aeruginosa...

  7. Los niveles de anticuerpos anti factor plaquetario 4-heparina y el índice 4T para trombocitopenia inducida por heparina

    Directory of Open Access Journals (Sweden)

    Marta E. Martinuzzo

    2012-02-01

    Full Text Available La trombocitopenia inducida por heparina (HIT es un efecto adverso del tratamiento con heparina, mediada por anticuerpos anti complejo factor plaquetario 4 (PF4-heparina (HPIA. La HIT es frecuentemente moderada pero pueden desarrollarse complicaciones trombóticas. El diagnóstico precoz es importante. La detección de HPIA por ELISA tiene alta sensibilidad pero baja especificidad (títulos bajos sin significación clínica. El índice de las 4T (índice 4T puede detectar pacientes con alto riesgo de HIT. El propósito del estudio fue correlacionar los niveles de HPIA y el índice 4T de un grupo de pacientes derivados a nuestro centro. Evaluamos 84 pacientes, 34 de ellos desarrollaron trombosis. Cada médico completó un cuestionario clínico que fue remitido con la muestra a nuestro centro. Los cuestionarios fueron analizados por un investigador externo y el índice 4T se calculó previamente al ensayo. Los HPIA se determinaron por un ELISA (Asserachrom HPIA que detecta los 3 isotipos, IgG, IgM e IgA, único reactivo disponible en Argentina. Los resultados se expresaron como porcentaje de absorbancia (%ABS. La correlación del índice 4T con los HPIA fue 0.472 (rho spearman, p < 0.001. Los pacientes con índice 4T ≥ 6 presentaban %ABS mayores que los ≤ 5 (67 vs. 39, p < 0.001. Aquéllos con trombosis presentaron títulos mayores que los que no la desarrollaron (%ABS 59 vs. 39, p = 0.017. En conclusión: Los títulos altos de HPIA medidos por ELISA, que detecta los 3 isotipos, correlacionaron claramente con el índice 4T ≥ 6 y fueron más frecuentes en los pacientes con trombosis, coincidiendo con lo ya descripto para ensayos de ELISA específicos para isotipo IgG.

  8. Efecto de la vacunación anti-leptospira en bovinos lecheros sobre la producción de anticuerpos anti-leptospira en trabajadores de lecherías de cuatro zonas de Costa Rica

    Directory of Open Access Journals (Sweden)

    Rafael González

    2007-07-01

    Full Text Available Objetivo: Este estudio experimental midió el efecto indirecto de la vacuna anti-Leptospira Leptoferm-5® (Pfizer® en la producción de anticuerpos anti-Leptospira en trabajadores de lecherías de cuatro zonas lecheras de Costa Rica. Materiales y Métodos: Tomaron parte en el estudio 171 vaqueros, 94 en el grupo tratamiento y 77 en el grupo control. A cada vaquero se le tomó una muestra sanguínea en tres diferentes momentos durante un año: la primera, antes de aplicar la vacuna en los animales, la segunda a los 6 meses y la tercera un año después de la primera vacunación. Se utilizó la técnica de micro aglutinación para la detección de anticuerpos anti-Leptospira interrogans serovares pomona, hardjo, canícola y grippotyphosa en el laboratorio del INCIENSA. Se utilizó la razón de prevalencias y el riesgo relativo para determinar si existió asociación entre la vacunación anti-Leptospira a las vacas y la seropositividad en los vaqueros. Resultados: En los tres muestreos se obtuvo un 10.27% (50/487 de muestras positivas a cualquiera de los serovares de Leptospira interrogans a los que fueron analizados. Tanto en el grupo tratamiento como en el control se observaron cambios en la seropositividad entre los distintos muestreos, con un aumento entre el primer y segundo muestreo (p Objective: This experimental study assessed the indirect effect of the anti-Leptospira vaccine Leptoferm-5® (Pfizer® regarding the production of anti-Leptospira antibodies in dairymen from four dairy areas in Costa Rica. Materials and Methods: 171 dairymen took part in the study; 94 in the treatment group and 77 in the control group. Three blood samples were taken from each worker three times throughout a year period: before the vaccination of the animals, and six and twelve months after the initial vaccination. The microscopic agglutination test (MAT was used to detect anti-Leptospira interrogans antibodies, specifically towards pomona, hardjo, canicola

  9. [The management of dialysis patients seropositive for HBsAg, anti-HCV, or anti-HIV antibodies].

    Science.gov (United States)

    Fabrizi, Fabrizio; Messa, Piergiorgio

    2012-01-01

    Infections by hepatitis B or hepatitis C virus are still common among patients on maintenance dialysis in Western countries. The natural history of HBV and HCV in the dialysis population remains unclear; however, there is good evidence showing an adverse impact of an anti-HCV seropositive status on survival in dialysis patients. A recent meta-analysis of observational studies (n=7, 11,589 unique patients) reported that anti-HCV-positive patients on dialysis had a higher mortality rate than those who were anti-HCV negative (adjusted hazard ratio=1.35, 95% confidence interval, 1.13; 1.59, pdialysis. Standard precautions and specific procedures against the transmission of blood-borne agents have been recommended to control HCV infection within dialysis units. Isolation by dialysis machines, staff and rooms has been strongly recommended to control HBV. Vaccination is an important tool against transmission of HBV infection among patients on maintenance dialysis; however, the immune response towards the hepatitis B vaccine in uremic patients remains unsatisfactory. Monotherapy with lamivudine is currently used for dialysis patients with hepatitis B whereas combination antiviral therapy (pegylated interferon plus ribavirin) is the standard of care for hepatitis C in the dialysis population, even if various side effects have been observed.

  10. Toxocara seroprevalence in children from a subtropical city in Argentina Prevalencia de anticuerpos anti Toxocara en niños de una ciudad subtropical de Argentina

    Directory of Open Access Journals (Sweden)

    José Mario ALONSO

    2000-08-01

    Full Text Available Most studies from Argentina have focused on toxocariasis as an environmental problem of big cities, and there are no available data about children infection from small or middle-sized cities. In order to assess the prevalence of anti-Toxocara antibodies in infantile population, 206 children from Resistencia, of both sexes, aged 1-14 years old were studied by Elisa testing with E/S T. canis L2 antigens. Hematological parameters and immunoglobulin levels were determined; five days' stool samples were studied and epidemiological data were obtained by means of a questionnaire to parents. Results showed that 73% of the children had one or more dogs living at home, 57% reported geophagia and 37.9% were positive for Toxocara serology, but there was no significant difference in prevalence neither for boys and girls, nor concerning age. An increased risk of infection was observed in age groups 5-6 and 7-8 for boys, and in age groups 3-4 and 5-6 for girls.La mayoría de los estudios realizados en Argentina, han enfocado a la Toxocariosis como un problema medioambiental propio de las grandes ciudades y no existen datos acerca de esta infección entre los niños de ciudades medianas o pequeñas. A fin de determinar la prevalencia de anticuerpos anti-Toxocara en la población infantil de la ciudad de Resistencia, se estudiaron 206 niños de ambos sexos, con edades comprendidas entre los 1-14 años, mediante el test de Elisa, empleando antígenos E/S de larva L2 de Toxocara canis. También se evaluaron los niveles de inmunoglobulinas séricas y los parámetros hematológicos; se efectuaron análisis coproparasitológico seriados en heces de 5 días y se recolectó información epidemiológica mediante cuestionario a los padres. Los resultados obtenidos señalan que el 73% de los niños tenían uno o mas perros viviendo en la casa, que el 57% tenían antecedentes de geofagia y que el 37.9% tenían serología positiva para Toxocara, aunque no hubo diferencias

  11. Effect of Revaccination Using Different Schemes among Adults with Low or Undetectable Anti-HBs Titers after Hepatitis B Virus Vaccination▿

    OpenAIRE

    Lin, Chao-Shuang; Xie, Shi-Bin; Jing LIU; Zhao, Zhi-Xin; Chong, Yu-Tian; Gao, Zhi-Liang

    2010-01-01

    Our objective was to investigate the effect of various reimmunization schemes for hepatitis B in adults with low or undetectable anti-HBs titers. Over 2 years, 10 μg of Saccharomyces cerevisiae-recombinant hepatitis B virus (HBV) vaccine (synthesized in China) was used in at least one standardized scheme to immunize 2,310 healthy male and nonpregnant female adults. Of these, 240 subjects tested negative for hepatitis B markers. These 240 subjects were equally divided into 4 groups. The first ...

  12. 非竞争性ELISA法测定人源抗HBsAg Fab功能性亲和常数%Measurement of functional affinity of humanized anti-HBsAg Fab using non-competitive ELISA method

    Institute of Scientific and Technical Information of China (English)

    郑大勇; 罗荣城; 韩焕兴

    2004-01-01

    目的测定完全人源化基因工程抗体HBsAg Fab的亲和常数.方法采用非竞争性ELISA固相法,经确定最佳抗原包板浓度、最佳抗原包板时间及最佳抗原与抗体结合反应时间后,得到了HBsAg与抗体片段抗HBsAg Fab及完整抗体抗HBsAg IgG的抗原抗体结合反应曲线,计算出抗HBsAg Fab及抗HBsAg IgG的亲和常数.结果人源基因工程抗体抗HBsAg Fab的功能性亲和常数在107~108M-1水平,比完整抗HBsAg IgG仅仅小约1个数量级(108~109M-1).结论该基因工程抗体与抗原结合能力较强,为今后开发应用Fab进行生物导向治疗提供了理论基础.

  13. Infección oculta por el virus de la hepatitis B en hijos de madres positivas al HBsAg

    Directory of Open Access Journals (Sweden)

    Marité Bello-Corredor

    2016-04-01

    Full Text Available La infección oculta por el virus de la hepatitis B (IOB, se caracteriza por la presencia en suero o plasma del genoma viral (ADN-VHB y anticuerpos contra la proteína de la cápside (anti-HBc en ausencia del antígeno de superficie (HBsAg, marcador que tradicionalmente se emplea para identificar la presencia del virus. Con el objetivo de caracterizar la presencia de IOB en hijos de madres positivas al HBsAg, se estudiaron 291 muestras séricas de niños con la condición de ser HBsAg (- y anticuerpos anti-HBsAg (anti-HBs menores de 50 UI/L, conservadas en la seroteca del Laboratorio de Referencia Nacional de Hepatitis Virales. Se realizaron ensayos para determinar la exposición al virus (anti-HBc, a los sueros anti-HBc (+ se les realizó Reacción en Cadena de la Polimerasa en Tiempo Real (RCP-TR para determinar y cuantificar el ADN-VHB. La prevalencia de exposición al VHB (anti-HBc fue 16,8% (49/291. El ADN viral se cuantificó en el 14% (6/43 de los casos anti-HBc (+, observándose cargas virales que oscilaban entre 2,15 x 101 hasta 3,42 x 101 UI/mL. La prevalencia de la IOB para el total de los pacientes analizados fue 2,1% (6/291, considerada relativamente baja. No se encontró asociación significativa entre las variables sociodemográficas analizadas tales como: edad, sexo y provincia de procedencia. La IOB está presente en hijos de madres positivas al HBsAg, a pesar de la profilaxis contra la hepatitis B. Por lo tanto, se requiere de pesquisajes adecuados para detectar dicha entidad. Las implicaciones clínicas y epidemiológicas de la misma, requieren de un estrecho monitoreo y atención de estos pacientes. Este estudio se realiza por primera vez en Cuba y aporta conocimientos útiles para el diagnóstico, prevención y control de esta enfermedad en niños.

  14. Prevalencia de anticuerpos anti-Leptospira spp. en personas con exposición laboral en el departamento del Tolima / Prevalence of anti-Leptospira spp. antibodies among people with occupational exposure in Tolima Department

    Directory of Open Access Journals (Sweden)

    Blanca L. Guzmán-Barragán

    2016-05-01

    Full Text Available Resumen Objetivo: estimar la prevalencia de anticuerpos IgM contra Leptospira spp., mediante el Ensayo de Inmunoabsorción Ligado a Enzimas (elisa, en la población de riesgo laboral de 8 municipios del Tolima. Metodología: se obtuvieron muestras de sangre de 261 empleados, las cuales fueron analizadas mediante la técnica de elisa para la detección de anticuerpos IgM anti-Leptospira spp., seguido de mat y serotipificación. Resultado: se estimó una seroprevalencia del 25,29%, con una seroreactividad mayor en trabajadores de plantas de beneficio animal (34,2%, recolección de residuos sólidos (27,1% y trabajadores de acueducto y alcantarillado (14,8%. La actividad en plantas de beneficio animal se identificó como factor de riesgo de Leptospira spp. (OR=1,86. Los serovares identificados fueron L. Bratislava (16, Ballum (5, Tarassovi (3, Hebdomadis (2, Sejroe (2 y Icterhemorragiae (1. El municipio de Libano presento el mayor porcentaje de positividad (36,96%, seguido de Espinal y Guamo con 28,57% cada uno. Discusión: la evaluación del sistema de vigilancia indicó deficiencia en recursos y debilidades de los profesionales de la salud al desconocer los procedimientos, investigación, diagnóstico y notificación de la enfermedad. Conclusiones: la leptospirosis está presente en poblaciones de riesgo laboral en el Tolima y se hace necesario abordar esta problemática en la población de otros municipios y los animales transmisores de la enfermedad. / Abstract Objective: to estimate the prevalence of IgM antibodies against Leptospira spp. Using the enzyme-linked immunosorbent assay (elisa in a population at occupational risk from 8 municipalities of the Tolima department, Colombia. Methodology: blood samples were collected from 261 employees and analyzed with the elisa technique to detect IgM and anti-Leptospira spp. antibodies. This was followed by mat and serotyping. Results: a seroprevalence of 25.29% was estimated, with higher

  15. Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance Caracterização de uma cepa de hepatite por vírus B no sudoeste do Paraná, Brasil, apresentando mutações previamente associadas à resistência anti-HBs

    Directory of Open Access Journals (Sweden)

    Dennis Armando Bertolini

    2010-02-01

    Full Text Available The present study investigated if hepatitis B virus (HBV mutants circulate in the southwestern region of the State of Paraná, Brazil, by analyzing samples from children who received immunoprophylaxis but were born to HBV carrier mothers. Samples from 25 children were screened for HBV serum markers and for HBV DNA by PCR. Only one sample was positive for HBsAg, anti-HBs and HBV DNA, although the child had been vaccinated. Analysis of the S gene sequence of this sample showed the presence of a proline at position 105, a serine at position 114, three threonines at positions 115, 116 and 140, and a glutamine at position 129. The presence of these amino acids, except for serine at position 114, has been related to monoclonal or polyclonal therapy with anti-HBs after liver transplantation, whereas the presence of threonine at position 116 has been described in immunized children from Singapore. This finding demonstrates the possible circulation of HBV strains resistant to hepatitis B immunoprophylaxis in southwestern Paraná, Brazil. The genotype of the sample was identified as genotype D, which is frequently found in the region studied. Since 36% of the children had received incomplete or no immunoprophylaxis, more extensive follow-up of children born to HBsAg-positive mothers is needed.O presente estudo investigou se mutantes do vírus da hepatite B (HBV circulam na região Sudoeste do Estado do Paraná, Brasil, analisando amostras de crianças que receberam a imunoprofilaxia por terem nascido de mães portadoras do HBV. Amostras de 25 crianças foram analisadas para os marcadores sorológicos do HBV e para o DNA-HBV por PCR. Somente uma amostra foi positiva para AgHBs, anti-HBs e DNA-HBV, apesar da criança ter sido vacinada. Análises da seqüência do gene S desta amostra mostrou a presença de uma prolina na posição 105, uma serina na posição 114, três treoninas nas posições 115, 116 e 140, e uma glutamina na posição 129. A presen

  16. Measurements in international units of antibody to hepatitis B surface antigen(anti-HBs) after immunization with a yeast-derived, subtype adr hepatitis B vaccine are considerably different between chemiluminescent immunoassay (CLIA) and chemiluminescent enzyme immunoassay (CLEIA).

    Science.gov (United States)

    Ogata, Norio

    2006-04-01

    The worldwide consensus of the minimum protective anti-HBs level against HBV infection is 10 mIU/mL on assays standardized by the World Health Organization (WHO) reference preparations. To investigate whether this value could be applied to recipients of yeast-derived recombinant HB vaccine containing the major surface protein of subtype adr (Bimmugen, Astellas Pharmaceutical, Tokyo), we compared anti-HBs measurements between chemiluminescent immunoassay (CLIA) (Architect Ausab, Abbott Japan, Tokyo) and chemiluminescent enzyme immunoassay (CLEIA) (Lumipulse Forte, Fujirebio, Tokyo) in given serum samples obtained from the vaccinees. The vaccine and the two assay methods are currently in a wide use in Japan. The study included 300 medical students who completed a standard vaccination course (0, 1 and 6 months). Serum samples obtained 1 month or 13 months after completing the vaccination were simultaneously tested for anti-HBs by CLIA and CLEIA. In 147 samples with quantifiable values on both CLIA and CLEIA (10 to 1000 mIU/mL) the geometric mean titer on CLEIA (225.0 mIU/mL) was significantly higher than that on CLIA (94.5 mIU/mL) (p < 0.0001). Of 26 subjects with CLIA measurements below 10 mIU/mL, 15 samples (57.7%) showed CLEIA measurements more than 10 mIU/mL. Thus, in the subtype adr-vaccinees CLEIA demonstrated considerably high serum anti-HBs measurements compared to CLIA and discordance in determining critical anti-HBs level of 10 mIU/mL was observed in more than half the samples. This suggests that the minimum HBV-protective anti HBs titer of 10 mIU/mL is difficult to be introduced to Japan where subtype adr-HB vaccines or -HBV infection are prevalent, unless characteristics of assay methods are carefully evaluated.

  17. Anti-HBs levels among children and adolescents with complete immunization schedule against hepatitis B virus. A cross-sectional study in Blumenau, State of Santa Catarina, Brazil, 2007-2008 Níveis de anti-HBs entre crianças e adolescentes com o esquema completo de imunização contra o vírus da hepatite B. Um estudo transversal em Blumenau, Estado de Santa Catarina, 2007-2008

    Directory of Open Access Journals (Sweden)

    Andréa do Livramento

    2011-08-01

    Full Text Available INTRODUCTION: Vaccination is the main tool for preventing hepatitis B virus (HBV infection; however, following the completion of the vaccination series, the concentrations of anti-HBs can decline over the years and reach levels less than 10mIU/mL. The persistence of protection in these individuals is still unknown. The present study aimed to determine the anti-HBs antibody levels among children and adolescents who had received a complete vaccination course for hepatitis B. METHODS: Antibodies against HBV surface antigen (anti-HBs were tested in 371 individuals aged 10 to 15 years-old. RESULTS: Volunteers who showed undetectable quantities of anti-HBs accounted for 10.2% of the population studied and 39.9% presented antibody titers of less than 10mIU/mL. Anti-HBs > 10mIU/mL were verified in 49.9%. CONCLUSIONS: These results corroborate other studies indicating levels of anti-HBs below 10mIU/mL in vaccinated individuals. Additional studies are required to assess whether this indicates susceptibility to HBV infection and the need and age for booster doses.INTRODUÇÃO: A vacinação é o principal instrumento para prevenir a infecção pelo vírus da hepatite B. Todavia, após a conclusão da série de vacinação, as concentrações de anti-HBs podem diminuir ao longo dos anos e atingir níveis inferiores a 10mUI/mL. A persistência da proteção nestes indivíduos ainda é desconhecida. O presente estudo objetivou determinar os níveis do anticorpo anti-HBs em crianças e adolescentes que receberam o esquema completo de vacinação para a hepatite B. MÉTODOS: O anticorpo para o antígeno de superfície do vírus da hepatite B (anti-HBs foi testado em 371 indivíduos com idade entre 10-15 anos. RESULTADOS: Os voluntários que apresentaram quantidades indetectáveis de anti-HBs corresponderam a 10,2% da população estudada, e 39,9% apresentaram títulos do anticorpo inferiores a 10mUI/mL. Anti-HBs > 10mUI/mL foi verificado em 49,9%. CONCLUS

  18. Presence of anti-HBc is associated to high rates of HBV resolved infection and low threshold for Occult HBV Infection in HIV patients with negative HBsAg in Chile.

    Science.gov (United States)

    Vargas, Jose Ignacio; Jensen, Daniela; Sarmiento, Valeska; Peirano, Felipe; Acuña, Pedro; Fuster, Felipe; Soto, Sabrina; Ahumada, Rodrigo; Huilcaman, Marco; Bruna, Mario; Jensen, Werner; Fuster, Francisco

    2016-04-01

    HBV-HIV coinfection is prevalent. Frequently, anti-HBc is the only serological marker of HBV, which can be indicative of HBV resolved infection, when found together with anti-HBs reactivity; or present as "isolated anti-HBc," related to HBV occult infection with presence of detectable DNA HBV, more prevalent in HIV-positive individuals. Regional data about this condition are scarce. Anti-HBc rapid test has been used as screening, but its performance has not been described in HIV-positive patients. The aim of this study was determine prevalence of anti-HBc in HIV-positive patients, serological pattern of HBV resolved infection and isolated anti-HBc, evaluating presence of HBV occult infection. Assess anti-HBc rapid test compared to ECLIA. Methods included measurement of anti-HBc and anti-HBs in HIV-positive patients with negative HBsAg. Serum HBV DNA quantification and HBV booster vaccination to "isolated anti-HBc" individuals. Detection of anti-HBc by rapid test and ECLIA. In 192 patients, prevalence of anti-HBc was 42.7% (82/192); associated to male gender, drug use, men-sex-men, positive-VDRL, and longer time HIV diagnosis. 34.4% (66/192) had presence of anti-HBs, mean titers of 637 ui/ml. Isolated anti-HBc in 8.3% (16/192), associated to detectable HIV viral load and no-use of HAART; in them, HBV DNA was undetectable, and 60% responded to HBV vaccination booster. Anti-HBc rapid test showed low sensibility (32.9%) compared to ECLIA. These results show that prevalence of anti-HBc in HIV-positive individuals is high, in most cases accompanied with anti-HBs as HBV resolved infection. Low prevalence of "isolated anti-HBc," with undetectable HBV DNA, and most had anamnestic response to HBV vaccination; suggest low possibility of occult HBV infection. Anti-HBc rapid test cannot be recommended as screening method for anti-HBc. PMID:26381185

  19. Encuesta sobre la realización de las pruebas de detección de los anticuerpos anti-VIH en los laboratorios de Cataluña Survey of HIV testing in laboratories in Catalonia [Spain

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    María Jesús Casado

    2004-02-01

    Full Text Available Para poder interpretar correctamente la epidemia de infección por el VIH, es indispensable garantizar la calidad en la realización del test anti-VIH. Con el objetivo de describir las características durante el proceso analítico e identificar aspectos susceptibles de mejora, en 1998 se realizó una encuesta sobre la garantía de calidad en los laboratorios de Cataluña que realizaban pruebas de detección de anticuerpos anti-VIH. Los resultados de la encuesta muestran la variabilidad de los procesos analíticos llevados a cabo por los diferentes laboratorios para la determinación diagnóstica de anticuerpos anti-VIH. Hay laboratorios que todavía realizan prácticas incorrectas, como identificar especialmente las muestras biológicas VIH, realizar segundas extracciones para pruebas de confirmación y no garantizar la confidencialidad en la entrega de resultados. Sería aconsejable unificar criterios según sea el objetivo de la prueba y la prevalencia de infección en la población analizada. Ello mejoraría la calidad del resultado analítico, ya que el concepto global de calidad incluye todo el proceso (preanalítico, analítico y postanalítico.Quality assurance of HIV testing is essential to correctly assess the HIV epidemic. To describe the characteristics of HIV testing and identify features that could be improved, a questionnaire on the quality of HIV tests was sent to Catalan laboratories in 1998. The survey revealed variability in the procedures used by the participating laboratories when performing HIV tests. Some of the laboratories were still performing incorrect activities such as identifying HIV specimens with specific labels, extracting new specimens for a second test to confirm diagnosis, or failing to guarantee the confidentiality of the results. The criteria for HIV testing should be standardized according to the purpose of the test and the prevalence of the infection in the population analyzed. This approach would improve

  20. The Expression of Humanized Fab Fragment of the Anti-HBsAg Antibody in Methylotropic Yeast Pichia pastoris%人源性抗HBsAg抗体Fab段在酵母中的表达

    Institute of Scientific and Technical Information of China (English)

    邓宁; 粟宽源; 王珣章; 龙綮新; 杨林; 余宙耀

    2002-01-01

    通过分步整合的方式,将人源性抗乙肝表面抗原(HBsAg)抗体Fab的轻、重链基因分步整合到巴斯德毕赤(Pichia pastoris)酵母GS115菌株的染色体上,经甲醇诱导,成功地分泌表达出抗HBsAg抗体的Fab片段,表达量达50~80mg/L.ELISA结果显示重组酵母分泌表达出的Fab具有较强的结合HBsAg的能力.通过抗Fab的抗体柱亲和层析,纯化出了纯度较高的Fab产品.

  1. [Evaluation of the analytic performance of blood collection tubes (BD Vacutainer SST) for the screening of anti-HIV, anti-HTLV, anti-HCV, anti-HBc, anti-CMV antibodies, and of HBs, P24 HIV antigens, and of alanine aminotransferase].

    Science.gov (United States)

    Gobin, E; Desruelle, J M; Vigier, J P

    2001-02-01

    The Laboratory of Viral Diseases Immunology (Laboratoire d'Immunologie des Maladies Virales) of the Northern Region Blood Bank (Etablissement Français du Sang Nord de France) performs between 180.000 and 200.000 viral blood qualifications per year. The use of a serum gel separator evacuated tube should contribute to improve the quality of the pre-analytical phase. However, it must not impact negatively the analytical performances. We evaluated such tube within our specific environment and with the various reagents used in routine. The open study compared the BD Vacutainer plain tube (7 mL, non siliconised) with the BD Vacutainer SST tube (6 mL siliconised with serum gel separator) against the anti-HIV, anti-HTLV, anti-HCV, anti-HBc, anti-HBs, anti-CMV antibodies, the HBs, HIV P24 antigen and the alanine aminotransferase. The study objectives were to find potential gel interference; to verify the diagnostic sensitivity, reagents specificity, and reproducibility. The results analysis show: equivalent performances with the anti-HIV Ab (Anti HIV 1/2 recombinant--Biotest et Genscreen HIV 1/2--Sanofi), anti HIV WB Ab (New Lav Blot 1--Sanofi), anti-HBs Ab (Enzygnost anti-HBs micro--Behring), anti-HBc Ab (HBc Elisa Test System--Ortho), anti-CMV Ab (Enzygnost anti-CMV IgG + M--Behring) kits; lower performances with: The Vironostika HIV Uni Form II plus 0--Organon kit with a -3.5% signal decrease around the ratio R = 2.7 for positive anti-HIV Ab. The Elisa test System 3 Ag HBs-Ortho kit with an increase of the mean ratio of the negative Ag HBs samples; better performances with: the Vironostika HIV 1 Antigen--Organon kit with a +10% signal increase around the threshold ratio R = 1 for positive Ag HIV samples. This deserves further study to verify that the specificity is maintained. The HTLV Type 1 et 2 EIA--Ortho kit with +8% signal increase around the ratio R = 2 for positive anti-HTLV Ab samples without change of the specificity. The Ortho HCV 3.0 Elisa Test System and

  2. The effects of booster vaccination on combined hepatitis A and hepatitis B vaccine in both anti-HBs and anti-HAV negative children 5-15 years after hepatitis B vaccine primary immunization.

    Science.gov (United States)

    Chen, Yongdi; Gu, Hua; Cheng, Suyun; Shen, Lingzhi; Cui, Fujiang; Wang, Fuzhen; Yao, Jun; Xia, Shichang; Lv, Huakun; Liang, Xiaofeng

    2013-04-01

    In the present study, we investigated the changes in both anti-HAV lgG and anti-HBs lgG levels and compared the antibody seroconversion rates of different doses of combined hepatitis A and hepatitis B vaccine in children. Children who were vaccinated as infants with Hepatitis B vaccine were revaccinated at 5-15 y of age, then the antibody titers were monitored. Among 283 children, this study found that the anti-HAV seroconversion rates (defined as anti-HAV ≥ 1 mIU/ml) after the first and the third dose were 79.9% and 100% respectively; these observed differences were statistically significant (P0.05). This study demonstrated that the immunization effects of booster vaccination with combined hepatitis A and hepatitis B vaccine is successful for children. A single booster dose is adequate for younger children, while three doses are needed for older children.

  3. 重组人抗HBsAg Fab抗体的纯化方法比较研究%Study on the purification of recombinant human anti-HBsAg Fab fragment

    Institute of Scientific and Technical Information of China (English)

    饶桂荣; 粟宽源; 陈文吟; 余宙耀

    2005-01-01

    目的研究重组人抗HBsAg Fab抗体的纯化条件.方法采用羊抗人Fab抗体亲和层析柱、14F7单克隆抗体亲和层析柱、离子交换-分子筛层析柱,分别纯化由酵母工程菌(GS115/Fab)发酵的重组人抗HBsAg Fab抗体,并对3种纯化方法所得Fab抗体的纯度、收率、与HBsAg的结合活性进行比较.结果 3种纯化方法中,14F7单克隆抗体柱纯化的Fab抗体的纯度达98%左右,Fab抗体柱纯化的Fab抗体的纯度为95%,但这两种亲和柱的目的蛋白收率都不高,分别为35%、55%.而离子交换柱纯化的Fab抗体的纯度为93.8%,经分子筛柱进一步纯化后,可达98%以上,Fab抗体蛋白收率可达80%以上.经ELISA分析,3种方法纯化的Fab抗体均具有较高的HBsAg抗原结合力和特异性.结论通过对3种纯化方法的比较得出,离子交换-分子筛层析法是重组人抗HBsAg Fab抗体的最佳纯化方法,这为抗HBsAg Fab抗体的产业化生产和临床研究打下良好基础.

  4. Determinación de las condiciones de ensayo óptimas en un ELISA para la detección de anticuerpos séricos IgG anti-LPS de Pseudomonas aeruginosa O11

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    Tania Valmaseda

    2004-12-01

    Full Text Available La selección de sueros de donantes sanos para la obtención de una gamma hiperinmune contra la infección por Pseudomona aeruginosa, así como la evaluación de la respuesta inmunológica de cualquier candidato vacunal contra este microorganismo necesita contar en el laboratorio con técnicas estandarizadas. En este trabajo se realizaron los ensayos necesarios para el montaje y la optimización de un ELISA indirecto para la determinación de anticuerpos séricos de clase IgG anti- LPS de P. aeruginosa O11. Se evaluaron la concentración de recubrimiento, condiciones de bloqueo, dilución de trabajo de las muestras a evaluar y del conjugado con el fin de seleccionar en cada caso las mejores respuestas para el control positivo, el control negativo y el blanco del ensayo. Una concentración de 1,5 μg/mL de LPS O11 en PBS toda la noche a 4 °C como recubrimiento, la necesidad de no incluir un paso adicional de bloqueo y el conjugado humano anti IgG-HRP diluido 1:3000, 1 h a 37 °C resultaron las variables óptimas para el ensayo. Por otra parte, se estableció el rango lineal de la curva del control positivo y se seleccionó la dilución de trabajo 1:100 para las muestras de sueros a evaluar.

  5. THE DETECTION OF ANTI-HBs,ANTI-HBc,AND HBV-DNA OF 2 274 BLOOD DONORS WITH HBsAg NEGATIVE%2 274名HBsAg阴性献血员抗-HBs、抗-HBc、HBV-DNA检测结果分析

    Institute of Scientific and Technical Information of China (English)

    许顺姬

    2007-01-01

    [目的]检测2 274名HBsAg阴性献血员血清抗-HBs、抗-HBc、HBV-DNA,探讨献血员筛查中检测抗-HBs、抗-HBc的必要性.[方法]对2 274名HBsAg阴性献血员血清ELISA法检测抗-HBs、抗-HBc,用PCR法检测HBV-DNA.[结果]检测者2 274名中①抗-HBs阳性/抗-HBc阴性59.3%,HBV-DNA阳性率0.2%;②抗-HBs阴性/抗-HBc阴性17.4%,HBV-DNA阳性率0.3%;③抗-HBs阴性/抗-HBc阳性1.4%,HBV-DNA阳性率6.3%:④抗HBs阳性/抗-HBc阳性21.9%,HBV-DNA阳性率0.2%.[结论]抗-HBs阴性/抗-HBc阳性群体中HBV-DNA阳性率最高(6.3%),提示献血员筛查中检测抗-HBs、抗-HBc,可以降低通过输血途径的感染率.

  6. 慢性乙型肝炎患者血清表面抗原与抗体同时阳性结果分析%Analysis of coexistence of hepatitis B surface antigen and anti - HBs antibodies in patients with chronic hepatitis B virus infection

    Institute of Scientific and Technical Information of China (English)

    陈天宝; 范公忍; 李娟; 胡学玲

    2011-01-01

    Objective:To study the relationship between concurrence mode of hepatitis B surface antigen and anti - HBs antibodies in patients with chronic hepatitis B virus infection and HBV DNA. Methods :Serological diagnosis for HBV infection was detected by VITROS Eci/ECiQ immunodiagnostic systems and HBV DNA viral load was quantified by using the commercially a-vailable real -time fluorescence quantitative kit ( PG Biotech). Results:Among the 5717 patients monitored at our hospital, 248 (4.34% ) have been identified as carrying both HBsAg and anti - HBs antibodies. In which, the majority appeared with HBV DNA at the same time. Conclusion: Coexistence of HBsAg and anti - HBs is not rare. HBV DNA concentration is an important parameter to diagnose and treat patients with simultaneous positive for HBsAg and anti - HBs antibodies.%目的:探讨乙型肝炎患者HBsAg和抗HBs共存模式及与HBV DNA的关系.方法:采用增强化学发光法检测血清乙肝标志物,并采用荧光定量PCR法检测HBV DNA.结果:5717例慢性乙肝患者检测出HBsAg和抗HBs双阳性248例,占4.34%,其中多数与HBV DNA同时出现.结论:HBsAg和抗HBs同时阳性并不少见,慢性乙肝患者出现抗HBs,不完全代表病毒复制终止,需结合HBV DNA定量综合分析.

  7. Expression of human anti-HBsAg single chain Fab gene in Pichia pastoris%人源性抗HBsAg单链Fab基因在Pichia pastoris中的表达

    Institute of Scientific and Technical Information of China (English)

    邓宁; 向军俭; 粟宽源; 王宏; 唐勇

    2006-01-01

    目的:研究重组Fab的结构与亲和活性的关系.方法:通过重叠PCR,将人源性抗HBsAg Fab的H和L链基因融合构建单链Fab基因,并将其转入毕赤酵母表达载体pPICZαA中.以单链Fab基因表达载体通过氯化锂转化法转化毕赤酵母GS115.将获得的重组酵母在摇瓶中培养进行重组单链Fab的可溶性表达.表达上清经硫酸铵沉淀及亲和层析纯化后,用直接ELISA检测表达产物和纯化Fab的活性.结果:SDS-PAGE和Western blot分析显示,单链Fab在毕赤酵母中获得分泌型表达.薄层扫描显示,在摇瓶中培养毕赤酵母表达的单链Fab约为5~10 mg/L.经亲和层析纯化获得纯度达97.8%的重组单链Fab.经直接ELISA测定的结果显示,重组单链Fab具有较好的结合HBsAg的活性.结论:通过重叠PCR构建的融合单链Fab基因,可成功地在毕赤酵母中获得分泌型表达,表达产物具有较好的结合HBsAg的活性.

  8. 重组人源性抗HBsAg Fab抗体纯化方法的比较研究%Study on the Methods of Purification of Recombinant Humanized anti-HBsAg Fab Antibody

    Institute of Scientific and Technical Information of China (English)

    邓宁; 向军俭; 饶桂; 陈文吟

    2004-01-01

    HBsAg Fab抗体被认为在预防和治疗HBV引起的肝病中具有重要的作用.为了建立稳定的、适于生产应用的重组人源性抗HBsAg Fab抗体的纯化工艺,本实验对不同纯化方法进行了比较研究.比较了抗Fab抗体亲和层析、ScFv单克隆抗体亲和层析和离子交换层析等3套纯化工艺在酵母发酵生产的重组人源性抗HBsAg Fab抗体纯化中的效率.结果显示,抗Fab抗体亲和层析柱纯化酵母表达的重组Fab,纯度为96.8%,但回收率偏低,只有30%~40%.ScFv单克隆抗体亲和层析纯化的重组Fab,纯度为97.5%,回收率达75%~85%.该工艺能很好的适应较小规模的生产应用.离子交换层析纯化的重组Fab,纯度为97%,回收率为75%~85%.该工艺能很好的适应较大规模的生产应用.以上结果表明,应用ScFv单克隆抗体亲和层析和离子交换层析纯化技术均能很好的纯化出重组人源性抗HBsAg Fab抗体,这两种纯化工艺不仅大大节约纯化成本,且纯化效率和回收率有很大提高.为重组Fab抗体的工业化生产应用奠定了基础.

  9. Level of Anti-HBs Among Children Aged Between 1 and 15 Years in Zhuzhou%株洲市1~15岁儿童乙型病毒性肝炎抗体水平

    Institute of Scientific and Technical Information of China (English)

    唐雨新; 龙术国; 任重

    2011-01-01

    目的 了解株洲市1~15岁儿童抗-HBs水平,评价儿童乙肝疫苗预防接种效果.方法 用单纯随机抽样方法 抽取1~15岁儿童288人,调查既往乙肝疫苗免疫史,每人抽静脉血检测乙肝抗-HBs.分析各年龄段乙肝免疫史与抗体滴度均数、抗-HBs阳性率的关系.结果 288名1~15岁儿童乙肝抗-HBs阳性率为74.31%,有免疫史人群抗-HBs阳性率明显高于无免疫史人群(P<0.01);1~岁、6~15岁年龄组抗-HBs阳性率差异有统计学意义(P<0.05),抗体滴度均数差异无统计学意义(P>0.05);1~15岁儿童乙肝全程接种率为87.15%,其中1~岁为87.22%,6~15岁为87.04%.结论 株洲市1~15岁儿童乙肝全程接种率高,儿童乙肝保护性抗体(抗-HBs)亦处于较高水平,但应在进一步提高乙肝疫苗接种率和首针及时接种率同时,探索适合低/无应答人群的免疫策略,以提高人群免疫水平.%Objective To investigate the level of anti - HBs in children aged between 1 and 5 years, and to evaluate the effect of HBV vaccine in these children. Methods With the simple random sampling method, 228 children aged 1~ 15 years were selected to survey their histories of hepatitis B vaccine inoculation. Their venous blood samples were collected to test anti - HBs antibodies. The correlation between the histories of hepatitis B vaccine inoculation and the average antibody titer, the anti - HBs positive rate in different age- groups was analyzed. Results Among the 288 children, the prevalence rate of HBsAb was 74.31%. The anti - HBs positive rate of children with histories of hepatitis B vaccine inoculation was significantly higher than that of children without the histories (P<0.01). There were statistically significant differences in the anti - HBs positive rate between the 1~ age group and the 6~15 age group (P<0.05), but no statistically significant difference was found in the average antibody titer between the two groups (P>0.05). The

  10. Intestinal helminthes and/or Toxocara infection are unrelated to anti-HBs titers in seven-year-old children vaccinated at birth with recombinant hepatitis B vaccine Helmintos intestinais e/ou infecção por Toxocara não tem relação com títulos de anti-HBs em crianças de sete anos de idade vacinadas ao nascer com vacina recombinante para hepatite B

    Directory of Open Access Journals (Sweden)

    Marisa B.C.L. Monteiro

    2007-04-01

    Full Text Available The aim of this investigation was to evaluate the possible effect of nematode infection on anti-HBs antibody levels in the serum of seven-year-old schoolchildren vaccinated at birth with the recombinant hepatitis B vaccine. Anti-HBs and anti HBc antibodies were evaluated in the sera of 100 schoolchildren with at least one intestinal nematode and/or a positive serological reaction for anti-Toxocara antibodies and in 95 schoolchildren without intestinal helminthiasis or serum anti-Toxocara antibodies. Both groups were from public elementary schools located on the urban periphery of Vitória, ES, Brazil. Among these 195 children, the median anti-HBs antibody titer was 31.3IU/ml and the frequency of titers less than 10IU/ml was 33.8% (95% CI: 27.1-40.4%. There were no significant differences between the medians of anti-HBs titers or the frequency of titers less than 10IU/ml between the groups with or without helminthes (29.5 and 32.9IU/ml and 33 and 34.7%, respectively; p>0.05. Even when the children with intestinal nematodes and/or anti-Toxocara antibodies and with blood eosinophil counts over 600/mm³ were compared with children without infection from intestinal nematodes and without anti-Toxocara antibodies, with blood eosinophil counts less than 400 eosinophils/mm³, these differences were not significant. None of the children presented anti-HBc antibodies. In conclusion, infections with intestinal nematodes and/or the presence of anti-Toxocara antibodies did not interfere with the anti-HBs antibody titers in seven-year-old children vaccinated at birth with the recombinant hepatitis B vaccine.O objetivo dessa investigação foi avaliar um possível efeito de infecções por nematóides sobre os níveis de anticorpos anti-HBs no soro de escolares de sete anos de idade, vacinados ao nascer com a vacina recombinante para hepatite B. Anticorpos anti-HBs e anti-HBc foram avaliados no soro de 100 escolares portadores de pelo menos um nemat

  11. Trasplante renal en pacientes con anticuerpos dirigidos contra donante

    Directory of Open Access Journals (Sweden)

    Federico Cicora

    2014-10-01

    Full Text Available En trasplante renal, los anticuerpos donante-específicos por ensayos de fase sólida predicen el rechazo temprano mediado por anticuerpos, incluso con resultados negativos de citometría de flujo o citotoxicidad dependiente del complemento. Aquí se describen los protocolos de inmunosupresión y los resultados a diez meses de cuatro pacientes en los que se detectó anticuerpos donante-específicos anti-antígenos leucocitarios humanos (HLA por Luminex®, pero no detectados por el método de citotoxicidad dependiente de complemento (CDC ni por citometría de flujo. Los cuatro pacientes recibieron tratamiento de inducción con 5 dosis de timoglobulina de 1.25 mg/kg y 5 dosis de inmunoglobulina intravenosa (IVIG de 400 mg/kg. Además, uno recibió 20 mg de basiliximab el mismo día del trasplante y el día 4 postrasplante; otro recibió 3 sesiones de plasmaféresis en los días -5, -3, y -1 y eculizumab en dosis de 1200 mg antes del trasplante, 900 mg el día 1, and 600 mg por semana durante un mes. En todos los casos, la inmunosupresión de mantenimiento consistió en tacrolimus, micofenolato y deltisona. Todos presentaron buenos resultados en el corto plazo. Nuestra experiencia sugiere que los pacientes con anticuerpos donante-específicos anti-HLA detectados solo por Luminex® deben recibir un seguimiento estricto y que en esta población se pueden obtener buenos resultados a partir del uso de terapia de inducción con timoglobulina e IVIG.

  12. Anticuerpo anticitrulina y manifestaciones extra articulares en artritis reumatoidea

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    María Jezabel Haye Salinas

    2013-02-01

    Full Text Available Los pacientes con artritis reumatidea (AR pueden desarrollar manifestaciones extra articulares (MExA, relacionadas a su morbi-mortalidad. Los anticuerpos anti-péptidos citrulinados cíclicos (ACCP son específicos para la AR y estan relacionados con el daño articular; y podrían tener rol patogénico en las MExA. Nuestro objetivo fue determinar la relación entre los anticuerpos ACCP y MExA en pacientes con AR. Se incluyeron 74 pacientes con diagnóstico de AR (ACR 1987 mayores de 18 años, de más de 6 meses de evolución, con MExA, y un control apareado por sexo y edad sin MExA por cada paciente. Las variables demográficas, clínicas y de laboratorio se compararon con test t, chi cuadrado o Mann-Whitney. Se realizó análisis multivariado; p ≤ 0.05. Los pacientes con MExA presentaron mayor título de anticuerpo ACCP (116 vs. 34, p < 0.01 y de factor reumatoideo (FR (108 vs. 34.5, p < 0.01. En el análisis multivariado hubo asociación entre la presencia de MExA y tabaquismo activo (p = 0.02, OR: 3.78, IC 95%: 1.17-12.2, FR positivo (p = 0.04, OR: 3.23, IC95%: 1.04-11.8 y anticuerpo ACCP positivo (p = 0.04, OR: 3.23, IC 95%: 1.04-10. Presentaron mayor título de anticuerpo ACCP que los controles los pacientes con xerostomía (109 vs. 34, p = 0.04, xeroftalmia (150 vs. 34, p < 0.01, nódulos sub-cutáneos (NSC (141 vs. 34, p < 0.01 y fibrosis pulmonar (158 vs. 34, p = 0.04. En conclusión, el anticuerpo ACCP positivo, el FR positivo y el tabaquismo activo fueron factores de riesgo independientes para el desarrollo de MExA.

  13. Rabbit IgG antibodies against Phospholipase A2 from Crotalus durissus terrificus neutralize the lethal activity of the venom Los anticuerpos IgG de conejos anti-fosfolipasa A2 de Crotalus durissus terrificus neutralizan la actividad letal del veneno

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    Juan P. Rodríguez

    2006-12-01

    Full Text Available Crotalus durissus terrificus (C.d.t. (South American rattlesnake venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2 and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutralize the lethality of the whole venom. PLA2 was isolated by gel filtration chromatography (Sephadex G-75. Specific antibodies were obtained by subcutaneous and intramuscular inoculation of PLA2 (700 µg with Freund adjuvant. Groups of six mice (20 + 2 g were inoculated with 0.5 ml i.p. of C. d. t. venom (4 µg or a mixture of venom that had been preincubated with the desired volume of IgG antibodies. Mortality, recorded 24 and 48 h after inoculation, showed that IgG anti-PLA2 were more effective than anticrotalic serum in neutralizing the lethal activity. These results demonstrate that it could be possible to obtain an anti-venom made by specific antibodies with a high level of protection against the lethal component of C.d.t. venom, and/or the inclusion of these antibodies as a supplement in heterologous anti-venoms.El veneno de Crotalus durissus terrificus (C.d.t. (Cascabel de Sud América posee actividad miotóxica y neurotóxica, actividades que también exhibe el complejo crotoxina, principal componente tóxico de este veneno. El complejo crotoxina está constituido por una fosfolipasa A2 básica (PLA2 y una proteína acídica no tóxica, el crotapotín. En este trabajo se estudió la capacidad neutralizante de anticuerpos IgG anti-PLA2 sobre la letalidad inducida por el veneno entero. El antígeno PLA2, fue aislado por cromatografía de filtración en gel (Sephadex G-75. Se inocularon conejos machos por vía subcutánea e intramuscular, con 700 µg de PLA2 y adyuvante para la obtención de anticuerpos específicos. La capacidad neutralizante del

  14. Morfología y respuesta de anticuerpos IgM e IgG anti-Blastocystis sp. en pacientes con síntomas gastrointestinales.

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    Emilia Elena Barrios

    2013-12-01

    Full Text Available Morphology and response of IgM and IgG anti - Blastocystis sp. in patients with gastrointestinal symptoms. It is important to define diagnostic criteria to elucidate the pathogenic role of Blastocystis sp . Morphology was assessed and diameter of the shapes, number of parasites per field and per gram of faeces and Blastocystis sp . Ig M and IgG antibodies was estimated by ELISA in subjects with constitutional symptoms an d healthy carriers. Seventy percent Blasctocystis sp positive samples were found, 52% from patients with symp toms such as flatulence 6 (24% , abdomi nal pain 5 (20 %, nausea 2 (8% , diarrhea 6 (24% and cons tipation 6 (24% . Ranges of parasites per field f ound more frequently in symptomatic patients were 0 - 3 (74% and 10 - 12 (26% , while 100% of asymptomatic patients had Blastocystis sp . in the range 0 - 3. Asymptomatic patients showed lower number of parasites per gram feces in the range of 0 - 3 and the 7 - 10 r ange with high parasitic load w as only observed in symptomatic . Less granular forms in both groups was found , in the range 0 - 3 in symptomatic patients : 85% vacuolar and 15% granular ; 76% vacuolar and 24 % granular in asymptomatic s . In the range 10 - 12, 98% v acuolar and 2% granular in symptomatic patients . The average diamet er of the vacuolar forms was 11. 9 ± 2 μm in s ymptomatic patients and 7.9 ± 3. 7 μm in asymptomatic s . In the granular form was 9.3 ± 2. 3 μm in symptomatic and 8.2 ± 0. 1 μm in asymp tomatics . Significant difference was found among vacuolar forms of both groups ( P = 0. 000026 , but not in the granular form (P = 0. 346 . Only 3. 5 % of s ymptomatic patients had IgG antibodies to Blastocystis sp. The m orphological study employed contributed to the diagn osis optimization while immunoenzymatic techniques were of low sensitivity.

  15. 国产抗-HBs定性试剂评估人群对乙型肝炎病毒的免疫状态%Evaluation of domestic qualitative reagents for detecting anti-HBs in assessing the immunity to hepatitis B virus

    Institute of Scientific and Technical Information of China (English)

    冯振华; 毕永春; 张姝; 陈洁; 胡娅莉; 周乙华

    2013-01-01

    目的:探讨国产抗-HBs定性试剂在评估人群对乙型肝炎病毒(HBV)有无免疫力的应用价值.方法:对1016例HBsAg阴性血清,分别用国产定性试剂和美国雅培定量试剂检测抗-HBs,比较结果的一致性和差异.结果:639例(62.9%)血清国产试剂检测抗-HBs阳性,742例(73.0%)雅培试剂检测阳性(≥10 mIU/ml,x2=23.98,P<0.001).在抗-HBs≥100 mIU/ml的444例、50 mIU/ml ~99 mIU/ml的83例和10 mIU/ml~49 mIU/ml的215例,国产试剂检测阳性率分别为99.1%、92.8%和52.1%.在雅培试剂检测抗-HBs<10 mIU/ml的274例中,国产试剂98.2%阴性.结论:国产抗-HBs定性试剂具有良好的特异性,虽然对低水平抗-HBs阳性血清的检出率较低,但抗-HBs ≥50 mIU/ml时,具有良好的检出率.国产定性试剂抗-HBs阳性,说明对HBV有免疫力,通常无需定量检测.%Objective:To assess the value of domestic qualitative reagents for anti-HBs in evaluating immunity to hepatitis B virus (HBV).Methods:A total of 1016 sera with negative HBsAg were tested for anti-HBs with domestic qualitative ELISA and Abbott quantitative reagents.The agreement and differences of the results with various reagents were statistically analyzed.Results:Of the 1016 sera,639 cases (62.9%) and 742 cases(73.0%) were positive for anti-HBs detected by the domestic reagents and Abbott quantitative reagents respectively (x2 =23.98,P <0.001).By detection with the domestic reagents,four hundred and forty (99.1%) of 444 sera with anti-HBs ≥100 mIU/ml and 77 (92.8%) of 83 sera with anti-HBs 50-99 mIU/ml were also positive.However,domestic qualitative reagents showed positive only in 112 (52.1%) of 215 sera with anti-HBs 10-49 mIU/ml.On the other hand,269 (98.2%) of 274 sera with anti-HBs < 10 mIU/ml were also negative detected with the domestic reagents.Conclusion:The domestic qualitative reagents for anti-HBs show high specificity and high agreement with Abbott quantitative reagents in

  16. Neuromielitis óptica con alta expresión de acuaporina-4 y anticuerpos anti-acuaporina-4 positivos en suero Neuromyelitis optica with high aquaporin-4 expression and positive serum aquaporin-4 autoantibodies

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    Alejandra Báez

    2012-04-01

    Full Text Available La presencia de anticuerpos IgG en suero, con blanco en los canales de acuaporina-4, es específica de la neuromielitis óptica (NMO. El 60% de los pacientes con NMO presentan lesiones cerebrales en la resonancia magnética (RM; en un 8% (mayoría niños estas lesiones se consideraron "atípicas". Presentamos dos pacientes con NMO y lesiones en el SNC de alta expresión de acuaporina-4. Caso 1: varón de 50 años, que comenzó con pérdida de visión en ojo derecho (OD. Recibió tratamiento empírico con metilprednisolona 1 g/d x 3 días. Al mes presentó dolor generalizado y hemiparesia derecha; nuevamente recibió metilprednisolona 1 g/d x 5 días e IgG IV 400 mg/kg/d × 5 días. Recuperó la deambulación persistiendo el dolor y fenómenos paroxísticos en los 4 miembros. Potenciales evocados visuales: P100, ojo izquierdo (OI 123 mseg. OD sin respuesta. La RM de cerebro (FLAIR mostró hiperintensidad en nervio óptico derecho, hipotálamo y comisura blanca anterior. RM cervical: lesión medular extensa (5 cuerpos vertebrales. Caso 2: mujer de 53 años, con disminución de la agudeza visual en ambos ojos y parestesias en miembros inferiores que remitieron espontáneamente. Evolucionó al mes con cuadriparesia e incontinencia esfinteriana. Recibió metilprednisolona 1 g/d x 5 días, sin mejoría. Potenciales evocados visuales: P100 OI 124 mseg. OD 128 mseg. RM cerebro: (FLAIR hiperintensidad hipotalámica y periacueductal. RM cervical: lesión medular extensa (7 cuerpos vertebrales. Anticuerpos anti-acuaporina-4 positivos en ambos pacientes (inmunofluorescencia indirecta. Las lesiones consideradas "atípicas", como aquí, en sitios con alta densidad de proteínas canales de agua AQP4 deberán considerarse para el diagnóstico diferencial.Disease-specific aquaporin-4 antibodies (NMO-IgG are the main effector of lesions in neuromyelitis optica (NMO patients. Brain MRI lesions are detected in 60% of them, with 8% (almost infants at sites of high

  17. Auto-anticorpos anti-β2-glicoproteína I e síndrome metabólica Anticuerpos anti-β2-glicoproteína I y síndrome metabólico Anti-beta2-glycoprotein I autoantibodies and metabolic syndrome

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    Rodrigo B. Krás Borges

    2011-04-01

    Full Text Available FUNDAMENTO: A síndrome metabólica (SM é uma entidade pró-aterogênica. Autoanticorpos tais como β2-glicoproteína I (β2-gpI podem influenciar o aparecimento de ateromas. Estudos anteriores confirmaram uma associação entre anticorpos IgA anti-β2-gpI e isquemia cerebral, infarto do miocárdio, doença arterial periférica e doença da carótida. OBJETIVO: O objetivo desse estudo de caso-controle foi avaliar uma possível associação entre anticorpos anti-β2-gpI e anticardiolipina (aCL com SM não-complicada. MÉTODOS: Pacientes com SM sem histórico de eventos vasculares e indivíduos-controle, consistindo em pacientes da Enfermaria de Ortopedia admitidos devido a doenças musculoesqueléticas foram incluídos no estudo. Idade, sexo, etnia, histórico de hipertensão, tabagismo, hipercolesterolemia e diabetes mellitus foram avaliados como fatores de risco em ambos os grupos. Anticorpos IgG, IgM, e IgA anti-β2-gpI e aCL foram detectados através de imunoensaios enzimáticos. RESULTADOS: Um total de 68 pacientes com SM e 82 controles foram estudados. Os pacientes com SM tinham média de idade superior à dos controles (P = 0,001, enquanto homens (P = 0,003; OR 0,31; IC95%: 0,15-0,16 e etnia caucasiana (P = 0,004; OR 0,25; IC95%:0,10-0,60 eram predominantes nos controles. Histórico de hipertensão, hipercolesterolemia e diabetes mellitus foi mais prevalente nos pacientes com SM do que nos controles (P FUNDAMENTO: El síndrome metabólico (SM es una entidad pro-aterogénica. Autoanticuerpos tales como β2-glicoproteína I (β2-GPI pueden influir en la aparición de ateromas. Estudios previos han confirmado una asociación entre anticuerpos IgA anti-β2-GPI y la isquemia cerebral, infarto de miocardio, enfermedad arterial periférica y enfermedad carotidea. OBJETIVO: El objetivo de este estudio de caso-control fue evaluar una posible asociación entre los anticuerpos anti-β2-GPI y anticardiolipina (aCL con SM complicada. MÉTODOS: Se

  18. Immunofluorescence detection of new antigen-antibody system (delta/anti-delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers.

    Science.gov (United States)

    Rizzetto, M; Canese, M G; Aricò, S; Crivelli, O; Trepo, C; Bonino, F; Verme, G

    1977-01-01

    A new antigen-antibody system associated with the hepatitis B virus and immunologically distinct from the HB surface, core, and e systems is reported. The new antigen, termed delta, was detected by direct immunofluorescence only in the liver cell nuclei of patients with HBsAg positive chronic liver disease. At present, the intrahepatic expression of HBcAg and delta antigen appears to be mutually exclusive. No ultrastructural aspect corresponding to the delta antigen could be identified under the electron microscope. delta antibody was found in the serum of chronic HBsAg carriers, with a higher prevalence in patients with liver damage. The nuclear fluorescence patterns of HBcAg and delta antigen were similar; it is only possible to discriminate between the two antigens by using the respective specific antisera. Images Figure PMID:75123

  19. Marcadores inflamatórios e anticorpos anti-chlamydia em pacientes com síndrome metabólica Marcadores inflamatorios y anticuerpos anti-chlamydia en pacientes con síndrome metabólico Inflammatory markers and antichlamydial antibodies in patients with metabolic syndrome

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    Rosecler Riethmuller Franco

    2011-02-01

    ão mostraram diferença significativa em pacientes com SM, com e sem eventos.FUNDAMENTO: El síndrome metabólico está asociado al aumento de riesgo de eventos cardiovasculares. Marcadores inflamatorios y anticuerpos anti-Chlamydia han sido relacionados al desarrollo y a la progresión de la aterosclerosis y de los eventos cardiovasculares. OBJETIVO: Evaluar los marcadores inflamatorios interleucina-6 (IL-6 y factor de necrosis tumoral-alfa (TNF-α y los anticuerpos anti-Chlamydia pneumoniae en pacientes con síndrome metabólico (SM, con y sin eventos cardiovasculares. MÉTODOS: Estudio transversal constituido por 147 individuos. De estos, 100 (68% con SM y sin eventos cardiovasculares; y 47 (32% con SM y con eventos cardiovasculares. De los individuos que habían sufrido eventos cardiovasculares, 13 (6,11% presentan infarto agudo de miocardio (IAM, y diez (4,7%, accidente cerebro vascular (ACV. El diagnóstico del SM fue determinado por los criterios del NCEP-ATPIII. RESULTADOS: La media de edad de los sujetos con eventos cardiovasculares fue de 61,26 ± 8,5 y de 59,32 ± 9,9 en los individuos sin esos eventos (p=0,279, habiendo predominio del sexo femenino. El grupo con SM y sin evento presentó mayor peso, altura, IMC y circunferencia abdominal. Para los individuos con eventos cardiovasculares (p=0,001, los marcadores inflamatorios IL-6 y TNF-α y la enfermedad vascular periférica fueron significativamente mayores. Se obtuvieron niveles elevados de anticuerpos IgG para Chlamydia pneumoniae en el grupo SM, sin eventos y de IgA en el grupo con eventos cuando fueron comparados los dos grupos. Con relación al IAM y al ACV, los anticuerpos anti-Chlamydia pneumoniae no demostraron significación estadística, comparados al grupo sin eventos cardiovasculares. Asociación fue observada con el uso de estatinas, hipoglicemiantes orales, inyectables y antiinflamatorios no esteroides en el grupo con esos eventos. CONCLUSIÓN: Marcadores inflamatorios se encuentran

  20. A Delayed Type Hypersensitivity (DTH) Skin Reaction to Hepatitis B Surface Antigen (HBsAg) and Intradermal Hepatitis B Vaccination

    OpenAIRE

    Nagafuchi, Seiho; Kashiwagi, Seizaburo; Hayashi, Jun; Katsuta, Hitoshi; Ikematsu, Hideyuki; Harada, Mine

    2004-01-01

    The significance of a delayed type hypersensitivity skin reaction to hepatitis B surface antigen (HBsAg) (HBs-DTH) in type B viral hepatitis (VHB) and in intradermal hepatitis B (HB) vaccination is reviewed. HBs-DTH could be developed by the intradermal injection of HB vaccine in anti-HBs positive people and also in persons immunized with HB vaccine. Thus, HBs-DTH could serve as a useful marker for the acquisition of an active Thl type immunoreactivity to HBsAg. HBs-DTH was absent in patients...

  1. 简阳地区乙型肝炎免疫水平研究%Analysis of Testing for Anti- HBs in 41490 peoples Cases

    Institute of Scientific and Technical Information of China (English)

    李阳超

    2013-01-01

    目的通过检测分析乙肝表面抗体(抗-HBs),了解本地区人群对乙肝的免疫水平,为预防乙肝提供科学依据.方法采用时间分辨免疫荧光分析法.结果41490例检出抗-HBs阳性20460例,阳性率49.31%;男性阳性率48.97%,女性阳性率49.68%,男女之比 P>0.05无显著性差异;0~9岁组阳性率为73.76%,与其余各年龄组之比,P0.05).The positive rate of 0-9 age group was 73.76%, there was significant difference (P<0.001) between the 0-9 age group and the other age groups. It was confirmed that all the peoples in this group were inoculated hepatitis B vaccination.The positive rate of HBsAb decreased with ages increase.Conclusion It is the most effective way to enhance the level of hepatitis B immunization by Increasing the rate of hepatitis B vaccine.

  2. Application of a newly developed high-sensitivity HBsAg chemiluminescent enzyme immunoassay for hepatitis B patients with HBsAg seroclearance.

    Science.gov (United States)

    Shinkai, Noboru; Matsuura, Kentaro; Sugauchi, Fuminaka; Watanabe, Tsunamasa; Murakami, Shuko; Iio, Etsuko; Ogawa, Shintaro; Nojiri, Shunsuke; Joh, Takashi; Tanaka, Yasuhito

    2013-11-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstrated seroclearance by the Abbott Architect assay. The performance of the Lumipulse HBsAg-HQ assay was compared with that of a quantitative HBsAg detection system (Abbott Architect) and the Roche Cobas TaqMan HBV DNA assay (CTM) (lower limit of detection, 2.1 log copies/ml) using blood serum samples from patients who were determined to be HBsAg seronegative by the Abbott Architect assay. Ten patients had spontaneous HBsAg loss. Of 8 patients treated with nucleotide analogues (NAs), two were HBsAg seronegative after stopping lamivudine therapy and 6 were HBsAg seronegative during entecavir therapy. Eight acute hepatitis B (AH) patients became HBsAg seronegative. Of the 26 patients, 16 were HBsAg positive by the Lumipulse HBsAg-HQ assay but negative by the Abbott Architect assay. The differences between the two assays in terms of detectable HBsAg persisted over the long term in the spontaneous loss group (median, 10 months), the NA-treated group (2.5 months), and the AH group (0.5 months). In 9 patients, the Lumipulse HBsAg-HQ assay detected HBsAg when HBV DNA was negative by the CTM assay. HBsAg was also detected by the Lumipulse HBsAg-HQ assay in 4 patients with an anti-HBs concentration of >10 mIU/ml, 3 of whom had no HBsAg escape mutations. The automatic, highly sensitive HBsAg CLEIA Lumipulse HBsAg-HQ is a convenient and precise assay for HBV monitoring.

  3. Long-term persistence of T cell memory to HBsAg after hepatitis B vaccination

    Institute of Scientific and Technical Information of China (English)

    Ru-Xiang Wang; Greet J. Boland; Jan van Hattum; Gijsbert C. de Gast

    2004-01-01

    AIM: To determine if the T cell memory to HBsAg can persist for a long time after hepatitis B (HB) vaccination.METHODS: Thirty one vaccine recipients who were healthcare workers (18 females and 13 males aged 34-58 years) from Utrecht University Hospital, Netherlands, and had previously Received a standard course of vaccination for hepatitis B were investigated and another 9 unvaccinated healthy volunteers from the same hospital were used as the control. Blood samples were taken just before the experiment to test serum anti-HBs levels and the subjects were classified into different groups according to their serum titers of anti-HBs and vaccination history. Their peripheral blood mononuclear cells (pBrvMc) were isolated from freshly heparinized venous blood and the proliferative response of Tlymphocytes to the recombinant hepatitis B surface antigen(HBsAg) was investigated.RESULTS: Positive serum anti-HBs was found in 61.3%(19/31) vaccine recipients and a significant in vitro lymphocyte proliferative response to recombinant HBsAg was observed in all the vaccinees with positive anti-HBs. Serum anti-HBs level ≤10 IU/L was found in 38.7% (12/31)subjects. In this study, we specially focused on lymphocyte proliferative response to recombinant HBsAg in those vaccine recipients with serum anti-HBsAg less than 10 IU/L.Most of them had Received a standard course of vaccination about 10 years before. T lymphocyte proliferative response was found positive in 7 of the 12 vaccine recipients. These results confirmed that HBsAg-specific memory T cells remained detectable in the circulation for a long time after vaccination, even when serum anti-HBs level had been undetectable.CONCLUSION: The T cell memory to HBsAg can persist for at least 10 years after HB vaccination. Further booster injection is not necessary in healthy responders to HB vaccine.

  4. UltramicroELISA para la detección de anticuerpos IgM anti M. leprae UltramicroELISA assay for the detection of human IgM antibodies to M. leprae

    OpenAIRE

    José Laferte; Elba G. Abreu; René Robaina; Vicente Verez

    1991-01-01

    La disponibilidad del sistema Ultramicroanalítico (SUMA) y de un antígeno especie-específico del M. leprae obtenido mediante síntesis química, permitió la normalización y validación de un ultramicroELISA para la detección de anticuerpos IgM específicos a esta micobacteria. El análisis de 433 sueros de banco de sangre y 265 sueros usados para validar el método y clasificados en un grupo control de donantes de banco de sangre (100), un grupo de pacientes tuberculosos (50), un grupo de enfermos ...

  5. Immunization with HBsAg-Fc fusion protein induces a predominant production of Th1 cytokines and reduces HBsAg level in transgenic mice

    Institute of Scientific and Technical Information of China (English)

    MENG Zhe-feng; WANG Hua-jing; YAO Xin; WANG Xuan-yi; WEN Yu-mei; DAI Jian-xin; XIE You-hua; XU Jian-qing

    2012-01-01

    Background The Fc receptor associated pathway might improve the immune responses against hepatitis B virus (HBV) as previously described by us.In addition,the Flt3 ligand (FL) has been reported to potentiate antigen presenting cells in vivo and may act as a potential adjuvant to boost antigen-specific immune responses.In this study,the immune efficacies of a set of fusion proteins of HBsAg and Fc and/or FL were evaluated in HBsAg transgenic mice.Methods The fusion proteins composed of HBsAg and the Fc domain of murine IgG1 (HBsAg-Fc) and/or the Flt3 ligand,and yeast-derived recombinant HBsAg were used as immunogen to immunize HBsAg transgenic mice,respectively.Serum and liver HBsAg levels,serum anti-HBsAg and cytokine profile,and the activities of alanine aminotransferase (ALT)/AST were investigated after immunization.Results After six injections,the most pronounced decrease in serum and liver HBsAg levels was observed in the HBsAg-Fc immunized group.In addition,serum Th1 cytokines and ALT/AST activities were highest in this group,indicating an effective induction of a favorable cellular immune response.Interestingly,the fusion protein containing HBsAg-Fc and the Flt3 ligand stimulated an alternative Th1-type immune response featured with high level productions of tumor necrosis factor α (TNF- α) and monocyte chemoabstractant protein 1 (MCP-1),causing a more severe cytotoxicity in hepatocytes while showed less effective in reducing serum HBsAg level.Conclusion HBsAg-Fc is effective in eliciting both the humoral and cellular immune responses against HBsAg in HBsAg transgenic mice,which makes it a potential immunogen for the immunotherapy of chronic hepatitis B.

  6. Thyroid autoantibodies in autoimmune diseases Anticuerpos antitiroideos en enfermedades autoinmunes

    Directory of Open Access Journals (Sweden)

    Regina M. Innocencio

    2004-06-01

    Full Text Available Abnormalities in the thyroid function and thyroid autoantibodies have been frequently described in patients with autoimmune diseases but seldom in antiphospholipid syndrome patients. In order to determine the prevalence of thyroid function and autoimmune abnormalities, we compared serum thyrotropin (TSH, serum free thyroxine (T4 levels, thyroid antithyroglobulin (TgAb and antithyroperoxidase (TPOAb levels of 25 patients with systemic sclerosis, 25 patients with rheumatoid arthritis and 13 patients with antiphospholipid syndrome to a control group of 113 healthy individuals. Evaluation included a thorough clinical examination with particular attention to thyroid disease and a serologic immune profile including rheumatoid factor, antinuclear and anticardiolipin antibody measurements. Subclinical hypothyroidism (4.2Ciertas anormalidades en la función tiroidea y anticuerpos antitiroideos han sido frecuentemente descriptos en pacientes con enfermedades autoinmunes, y más raramente en pacientes con el síndrome antifosfolipídico. Para determinar la prevalencía de anormalidades en la función tiroidea y de autoinmunidad, comparamos los niveles séricos de tirotropina (TSH tiroxina libre en suero (T4 anticuerpos antitiroglobulina (TgAb y antitiroperoxidasa (TPOAb en 25 pacientes con esclerosis sistémica, 25 pacientes con artritis reumatoidea y 13 pacientes con el síndrome antifosfolipídico con un grupo control de 113 individuos aparentemente sanos. La evaluación incluyó un completo examen clínico con particular atención para las enfermedades de la tiroides y una evaluación inmunológica incluyendo dosaje del factor reumatoideo, anticuerpos antinucleares y anticardiolipina. Hipotiroidismo subclínico (4.2anticuerpos anti-tiroglobulina (TgAb y/o anti-peroxidasa (TPOAb estaban presentes en 21/63 (33% de los

  7. Oral vaccination of mice with Tremella fuciformis yeast-like conidium cells expressing HBsAg.

    Science.gov (United States)

    Shin, Dong-Il; Song, Kyu-Seon; Park, Hee-Sung

    2015-03-01

    Tremella fuciformis yeast-like conidium (YLC) cells were transformed by co-cultivation with Agrobacterium cells harboring the hepatitis B surface antigen (HBsAg) gene construct under the control of the CaMV35S promoter. Integration of HBsAg DNA into the YLC genome was confirmed by PCR and dot-blot hybridization. Immunoblotting verified expression of the recombinant protein. Oral administration of YLC cells expressing HBsAg in mice significantly increased anti-HBsAg antibody titer levels using a double prime-boost strategy that combined parenteral and oral HBsAg boosters. PMID:25374008

  8. Prevalencia de anticuerpos anti-Chlamydia trachomatis y anti-Neisseria gonorrhoeae en grupos de individuos de la población mexicana Prevalence of antibodies against Chlamydia trachomatis and Neisseria gonorrhoeae in Mexican populations

    Directory of Open Access Journals (Sweden)

    María del Carmen Cravioto

    2003-01-01

    Full Text Available OBJETIVO: Estimar la prevalencia de infección por Chlamydia trachomatis (CT y Neisseria gonorrhoeae (NG en grupos de individuos con diferente riesgo para infecciones de transmisión sexual. MATERIAL Y MÉTODOS: Estudio transversal multicéntrico efectuado en el Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, de la Ciudad de México, de enero de 1992 a diciembre de 1993, en el que se estudiaron 945 individuos en edad reproductiva, 585 mujeres y 360 hombres. Según su riesgo para infecciones de transmisión sexual se clasificaron en grupos de alto y bajo riesgo. Los de alto riesgo incluyeron mujeres infértiles con daño tubario, mujeres con embarazo ectópico o aborto, hombres infértiles, pacientes con VIH/SIDA, hombres homo o bisexuales y trabajadoras del sexo comercial (TSC. Los de bajo riesgo: mujeres primigestas, hombres fértiles y mujeres infértiles sin daño tubario. Se determinó en duplicado IgG e IgA anti-NG y anti-CT en suero, por análisis inmunoenzimático, utilizando como antígeno el pili de NG y la fracción L1 de CT. Se calcularon porcentajes. RESULTADOS: En mujeres la prevalencia para NG fue IgG 13.7% e IgA 14.3%, y para CT fue IgG 11.4% e IgA 4.4%. En hombres, NG 3.3% y 13.3%, respectivamente, y para CT 7.2% y 5.5%, respectivamente. En TSC se encontró NG en 31.2 % y 28.4%, respectivamente, y para CT 25.0% y 5.7%, respectivamente. En mujeres con infertilidad por daño tubario, NG 5.6% y 9.8%, respectivamente, y CT 8.4% y 1.4%, respectivamente. En 110 primigestas jóvenes, NG 4.5% y 10.0%, respectivamente, y CT 3.6% y 9.1%, respectivamente. CONCLUSIONES: Estos datos confirman la prevalencia elevada de Neisseria gonorrhoeae y Chlamydia trachomatis en trabajadoras del sexo comercial y en hombres homo/bisexuales, pero no en otros grupos de alto riesgo como las mujeres infértiles, con aborto o embarazo ectópico.OBJECTIVE: To estimate the prevalence of Chlamydia trachomatis (CT and Neisseria gonorrhoeae

  9. The Preparation of Mice Ascites with Anti-HBs/a Monoclonal Antibody Hybridoma Cell Separated from Mice Ascites%分离小鼠腹水中抗HBs/a单克隆抗体杂交瘤细胞制备小鼠腹水

    Institute of Scientific and Technical Information of China (English)

    邹汉武; 刘琦

    2001-01-01

    探讨利用从小鼠腹水中分离获得的单克隆抗体杂交瘤细胞, 大量制备小鼠单克隆抗体腹水的方法:收集已接种单克隆抗体杂交瘤细胞的小鼠腹水,用淋巴细胞分离液离心分离腹水中的杂交瘤细胞;再将此分离出的杂交瘤细胞,注入其他小鼠腹腔又制得腹水。每只小鼠分离得到的杂交瘤细胞可供5只小鼠腹腔注射, 平均每只小鼠产腹水3.97 ml。此法可规模制备大量高效价的单克隆抗体腹水。%A large quantity of mice McAb ascites with monoclonal antibody (McAb) hybridoma cells separated from mice ascites was prepared. Ascites of mice inoculated with McAb hybridoma cells were collected, and the McAb hybridoma cells were isolated by centrifugation with lymphocyte separating fluid. McAb hybridoma cells then were injected into other mice abdominal cavity to prepare ascites again. McAb hybridoma cells separated from a mouse could be injected into the abdominal cavity of 5 mice. Each mouse could produce 3.97 ml ascites on average. This method could be used to prepare large quantity of high titer mice monoclonal anti-HBs/a ascites.

  10. Diagnóstico diferencial en la encefalitis por anticuerpos contra el receptor NMDA

    Science.gov (United States)

    González-Valcárcel, J.; Rosenfeld, M.R.; Dalmau, J.

    2011-01-01

    Resumen Introducción La encefalitis por anticuerpos contra el receptor de NMDA (NMDAR) suele desarrollarse como un síndrome característico de evolución multifásica y diagnóstico diferencial amplio. Pacientes Presentamos a 2 pacientes diagnosticadas de encefalitis por anticuerpos NMDAR con un cuadro clínico típico, pero que inicialmente señaló otras etiologías. Discusión La afectación frecuente de pacientes jóvenes con manifestaciones psiquiátricas prominentes indica frecuentemente otras consideraciones diagnósticas; las más frecuentes son las encefalitis virales, los procesos psiquiátricos y el síndrome neuroléptico maligno. Varios síndromes previamente definidos de manera parcial o descriptiva en adultos y pacientes pediátricos probablemente eran casos de encefalitis anti-NMDAR. Conclusiones La encefalitis anti-NMDAR debe considerarse en pacientes jóvenes con manifestaciones psiquiátricas subagudas, movimientos anormales y alteraciones autonómicas. La caracterización clínica e inmunológica de esta enfermedad ha llevado a la identificación de nuevos anticuerpos que afectan a procesos de memoria, aprendizaje, conducta y psicosis. PMID:20964986

  11. UltramicroELISA para la detección de anticuerpos IgM anti M. leprae UltramicroELISA assay for the detection of human IgM antibodies to M. leprae

    Directory of Open Access Journals (Sweden)

    José Laferte

    1991-12-01

    Full Text Available La disponibilidad del sistema Ultramicroanalítico (SUMA y de un antígeno especie-específico del M. leprae obtenido mediante síntesis química, permitió la normalización y validación de un ultramicroELISA para la detección de anticuerpos IgM específicos a esta micobacteria. El análisis de 433 sueros de banco de sangre y 265 sueros usados para validar el método y clasificados en un grupo control de donantes de banco de sangre (100, un grupo de pacientes tuberculosos (50, un grupo de enfermos de lepra (65 y un grupo de contactos de estos enfermos (50, mostró la especificidad del ensayo para evidenciar la infección con el M. leprae. Los resultados obtenidos del estudio adicional de 140 muestras de suero de contactos de enfermos estuvieron estrechamente correlacionados (r = 0,98 con los resultados obtenidos por la técnica de microELISA convencional. La utilización del SUMA no solo permite un notable ahorro de reactivos si no además facilita la lectura, cálculo, validación y almacenamiento automático de los resultados.The availability of an ultramicroanalitic system (SUMA and specie-specific antigen of M. leprae obtained by chemical synthesis, have made possible the standardization and validation of an ultramicroELISA assay for detecting specific human IgM antibodies to this mycobacterium. The specificity of this test to demonstrate the infection with M. leprae was corroborated through a screening of 433 blood bank serum samples and other 265 from diferent groups (100, control group, 50 tuberculosis patients, 65 leprosy patients, 50 from household. The results obtained in the aditional study of 140 household sero showed a high correlation (r = 0.98 with the conventional microELISA method. The use of SUMA allows saving reagents and time since sample handling, plate reading, print out and storing the data are computer assisted.

  12. HBsAg阳性母亲所生婴儿联合免疫后乙型肝炎表面抗体的动态变化%Combined immunoprophylaxis induces changes in anti-hepatitis B surface protein titer in infants born to mothers with positivity for hepatitis B surface antigen

    Institute of Scientific and Technical Information of China (English)

    王静; 冯玉岭; 刘明晖; 白云; 冯达红; 袁宁霞; 杜冬青; 冯卫红; 刘红莉

    2013-01-01

    Objective To conduct a prospective randomized controlled trial of infants born to hepatitis B virus (HBV) surface antigen (HBsAg)-positive mothers in order to investigate the dynamic changes in the titer of anti-HBV surface protein (HBS) induced by treatment with combined immunoprophylaxis (200 IU hepatitis B immunoglobulin (HBIG) and 5 or 10 μg yeast recombinant hepatitis B vaccine),to compare the protective effect of 5 and 10 μg hepatitis B vaccine,and to provide an immunization strategy,monitoring mode and booster immunization schedule for the high-risk group.Methods Two-hundred-and-sixty-nine infants born to HBsAg positive mothers were given combined immunoprophylaxis at birth,and the venous blood samples (at birth,and 1,7 and 12 months) were tested for HBV DNA load,and HBsAg and anti-HBS titers.Results The overall 1-year protective rate of combined immunoprophylaxis was 95.9%.There was no significant difference between the infectious rates of infants given the 5 μg or the 10 μg hepatitis B vaccine (x2 =0.876,P =0.377).The geometric mean titers (GMTs) of anti-HBS were 144.1 mIU/ml at 1-month old and 564.9 mIU/ml at the age of 7 months old (the highest point),but declined to 397.6 mIU/ml at the age of 12 months old.The rate of infants with anti-HBS titer < 100 mIU/ml was 20.9%,and that of< 10 mIU/ml was 7.4% at 7-month-old; the rate of infants with anti-HBS titer < 100 mIU/ml increased to 30.2% and that of < 10 mIU/ml increased to 15.9% at 12-monthold.At 7-month-old,the GMT of the 10 μg vaccine group was higher than that of the 5 μg vaccine group (675.3 mIU/ml vs.25.0 mIU/ml,P =0.001) and the rate of infants with anti-HBS titer < 10 mIU/ml was significantly lower in the 10 μg vaccine group (2.3% vs.12.6%,P =0.002); at 12-month-old,the rate of infants with anti-HBS titer < 100 mIU/ml was also significantly lower in the 10 μg group (20.6% vs.40.2%,P =0.001).Conclusion Combined immunoprophylaxis is therapeutically efficacious

  13. 乙型肝炎疫苗初次免疫成年无应答者再次免疫后24个月免疫持久性观察%Anti-HBs persistence after revaccination with three doses of hepatitis B vaccines among non-responsive adults:24-month of follow-up

    Institute of Scientific and Technical Information of China (English)

    张丽; 王富珍; 徐爱强; 吕静静; 颜丙玉; 刘甲野; 冯艺; 陈士玉; 周立波; 梁晓峰; 崔富强

    2015-01-01

    值为2.16(1.10~4.23)],女性高于男性[OR(95%CI)值为1.88(1.07~3.31)],T1时抗体GMC为50~99和≥100 mU/ml者高于T1时抗体GMC<50 mU/ml者[OR(95%CI)值分别为3.43(1.55~7.60)和21.26(12.12~37.29)];T24时,T1时抗体GMC为50~99和≥100 mU/ml者的抗体GMC高于T1时抗体GMC<50 mIU/ml者[β(95%CI)值分别为0.95(0.35~1.54)和2.90(2.54~3.25)]。T24时未发现HBsAg阳转者,抗-HBc阳转率为3.64%(17/467)。结论 HepB成年无应答者3剂次再次免疫24个月后抗-HBs阳性率和抗体GMC下降,但多数人仍维持在保护水平以上;无应答者再次免疫后的抗体持久性主要与疫苗种类、性别和再次免疫1个月时的抗体GMC有关。%Objective To access the antibody persistence 24-month after revaccination with 3-dose of hepatitis B vaccine (HepB) among non-response adults. Methods A total of 24 237 healthy adults who had no histories of hepatitis B infection and hepatitis B vaccination, resided in the local area for more than six months and were aged 18-49 years were selected from 79 villages of Zhangqiu county, Shandong province, China in 2009. Blood samples were obtained and hepatitis B surface antigen (HBsAg), antibody against hepatitis B surface antigen (anti-HBs) and antibody against hepatitis B core antigen (anti-HBc) were detected using ELISA method. A total of 11 590 persons who were negative for all of these indicators were divided into four groups by cluster sampling methods. Each group was vaccinated with one of the following four types of HepB at 0-, 1-, 6- months schedule: 20 μg HepB derived in Saccharomyces Cerevisiae (HepB-SC), 20 μg HepB derived in Chinese hamster ovary cell (HepB-CHO), 10 μg HepB-SC and 10 μg HepB derived in Hansenula Polymorpha (HepB-HP). Blood samples were collected one month after the third dose of primary immunization and tested for anti-HBs using chemiluminescence microparticle immunoassay (CMIA). The non-responders were revaccinated

  14. Relationship Between HBV-DNA and Pre S1 Antigen in Patients with HBsAg, Anti-HBe and Anti-HBc Positive Hepatitis B%乙型肝炎“小三阳”患者HBV-DNA定量检测与前S1抗原的关系

    Institute of Scientific and Technical Information of China (English)

    石万元; 阚秉辉; 刘克芹; 尹卫东

    2013-01-01

    目的 探讨“小三阳”患者HBV-DNA含量与前S1抗原的关系.方法 收集乙型肝炎“小三阳”患者血清152例,用ELISA方法检测前S1抗原,用实时荧光定量PCR(FQ-PCR)方法检测HBV-DNA.结果 前S1与HBV-DNA同时阳性者74例,二者同时阴性者34例,符合率为71.1%.前S1阳性、HBV-DNA阴性者为30例,HBV-DNA阳性而前S1阴性者为14例.即“小三阳”患者中前S1的检出率为68.4%,HBV-DNA的检出率为57.9%,并且拷贝数高低有差别.结论 “小三阳”患者体内也存在着不同程度的病毒复制.前S1的检测不能代替HBV-DNA,只能作为其补充指标.对于“小三阳”患者,同时检测HBV-DNA定量有助于判断病毒在体内是否复制.%Objective To investigate the relationship between HBV-DNA with pre S1 antigen in patients with HBsAg,Anti-HBe and Anti-HBc positive hepatitis B.Methods The serum express of pre S1 antigen and HBV-DNA in 152 patients with HBsAg,Anti-HBe and Anti-HBc positive hepatitis B were detected by ELISA and PCR(FQ-PCR) respectively.Results 74 cases of samples were both pre S1 and HBV-DNA positive,while 34 cases were found Pre S1 and HBV-DNA negative.The positive coincidence rate between Pre S1 and HBV-DNA was 71.1%.There were 30 cases with pre S1 positive and HBV-DNA negative,while 14 cases had HBV-DNA positive and pre S1 negative.The detection rate of pre S1 and HBV-DNA in patients with HBsAg,Anti-HBe and Anti-HBc positive hepatitis B were 68.4% and 57.9%,respectively.Conclusion There are virus replications of different degrees in patients with HBsAg,Anti-HBe and Anti-HBc positive hepatitis B.The detection of Pre S1 can not replace HBV-DNA detection,and it could be only used as a supplementary index.The HBV-DNA quantitative detection could facilitate to judge whether virus in-vivo replicate or not.

  15. Stabilization and immune response of HBsAg encapsulated within poly(lactic-co-glycolic acid) microspheres using HSA as a stabilizer.

    Science.gov (United States)

    Xu, Wenjuan; He, Jintian; Wu, Guanghao; Xiong, Fangfang; Du, Huijuan; Wang, Gaizhen

    2015-12-30

    The aim of this study was to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres containing hepatitis B virus surface antigen (HBsAg) using human serum albumin (HSA) as a stabilizer. Lyophilization and emulsification of HBsAg solution with dichloromethane caused a considerable loss of HBsAg antigenicity. Thus, the effects of HSA and trehalose on HBsAg recovery during lyophilization and emulsification were investigated. Adding HSA to HBsAg solutions significantly improved antigen recovery to >90% during lyophilization and emulsification. The effects of co-encapsulated HSA on the characteristics of the PLGA microspheres and stability of HBsAg released from the microspheres were also investigated. The in vitro release test showed that HBsAg was released from the PLGA microspheres continuously over seventy days. A large amount of released HBsAg was inactive without co-encapsulation of HSA. On the contrary, with HSA co-encapsulation, the released HBsAg retained approximately 90% of its antigenicity. The single injection of the HBsAg-HSA-loaded PLGA microspheres in rats resulted in higher anti-HBsAg IgG and Th1 cytokine levels than the single injection of the HBsAg-loaded microspheres or two injections of the conventional aluminum-adjuvanted HBsAg vaccine. Based on these findings, the HBsAg-HSA-loaded PLGA microspheres could be an effective carrier for HBsAg and form a promising depot system.

  16. Obtención de anticuerpos monoclonales de ratón contra proteasa de cisteína 5 recombinante de Entamoeba histolytica

    Directory of Open Access Journals (Sweden)

    Juanita Trejos S.

    2012-08-01

    Full Text Available Objetivo. Obtener anticuerpos monoclonales de ratón contra la proteasas de cisteína 5 (EhCP5 de Entamoeba histolytica. Materiales y métodos. Se inmunizaron ratones BALB/c por vía intraperitoneal con adyuvante de Freund completo e incompleto con la proteína recombinante EhCP5 obtenida a partir del cultivo de E.coli DH5α trasfectada con el vector recombinante pJC45 que expresa dicha proteína. Se seleccionó el animal con mejor respuesta de anticuerpos. Al cual se le extrajo su bazo como fuente de linfocitos B, los cuales se fusionaron utilizando PEG con células de mieloma de ratón SP2-0/Ag14. Se procedió a selección de los hibridomas y a la evaluación de los sobrenadantes de las colonias que crecieron a los 7 días mediante ELISA. Los hibridomas con valores más altos de anticuerpos específicos contra la proteína EhCP5r se seleccionaron, y los clones obtenidos por diluciones limitantes fueron expandidos. Resultados. A partir de un clon secretor estable se purifico el anticuerpo monoclonal anti EhCP5r del isotipo IgG1 por cromatografía de afinidad con proteína G. Los clones fueron expandidos in vivo e in vitro. Con el anticuerpo purificado se diseñaron tres sistemas de captura para evaluar la aplicabilidad del anticuerpo monoclonal anti EhCP5r como método inmunodiagnóstico. Conclusiones. Se logro la producción de un anticuerpo monoclonal específico contra EhCP5r que permite diferenciar Entamoeba histolytica de Entamoeba dispar.

  17. Inmunofluorescencia con Crithidia luciliae para la detección de anticuerpos anti-ADN: Imágenes atípicas y su relación con enfermedad de Chagas y leishmaniasis Immunofluorescence assay with Crithidia luciliae for the detection of anti-DNA antibodies: Atypical images and their relationship with Chagas’ disease and leishmaniasis

    Directory of Open Access Journals (Sweden)

    Gloria Griemberg

    2006-02-01

    Full Text Available Los anticuerpos anti-ADN nativo pueden detectarse por inmunofluorescencia indirecta con Crithidia luciliae, observándose tinción fluorescente anular del kinetoplasto que contiene ADN de doble cadena. En algunos casos pueden observarse imágenes fluorescentes en flagelo, membrana y corpúsculo basal, consideradas atípicas. Como C. luciliae pertenece a la familia Trypanosomatidae, que incluye patógenos para el hombre como Trypanosoma cruzi y Leishmaniaspp., se consideró que las imágenes atípicas pudieran deberse a reacciones cruzadas. Se realizaron estudios serológicos para Chagas a 105 muestras provenientes de zona endémica (Corrientes y no endémica (Buenos Aires para T. cruzi que presentaban imágenes atípicas con C. luciliae. La serología para Chagas resultó positiva en el 64.7% de las muestras de Buenos Aires y en el 78.3% de las de Corrientes que presentaban frente a C. luciliae imagen conjunta de membrana y flagelo. No presentaron la imagen conjunta ninguna de las muestras de dadores de sangre normales, ni de pacientes con enfermedades del tejido conectivo, excepto dos con lupus que también eran chagásicos. Todas las muestras de pacientes chagásicos analizadas frente a C. luciliae presentaron la imagen conjunta. Se estudiaron también 46 muestras de pacientes con leishmaniasis, 28 de ellos coinfectados con T. cruzi. La imagen conjunta se observó en el 88.0% de las muestras de leishmaniásicos y en el 78.5% de las de coinfectados. Los resultados sugieren que C. luciliae podría ser un sustrato alternativo, económico y de bajo riesgo para el diagnóstico serológico de enfermedad de Chagas, aunque no discrimina la infección por Leishmania. El hallazgo de la imagen conjunta en la detección de anti-ADN nativo señala la conveniencia de realizar en esos pacientes, estudios clínicos y de laboratorio para enfermedad de Chagas y leishmaniasis.Anti-native DNA antibodies can be detected by indirect immunofluorescence assay with

  18. 献血者HBsAg及抗-HCV ELISA筛查不合格标本的假阳性分析%Evaluation on the false positive rate of unqualified donors with positive HBsAg and anti-HCV ELISA screen test

    Institute of Scientific and Technical Information of China (English)

    宋美兰; 任芙蓉; 龚晓燕; 王卓妍

    2013-01-01

    目的 了解献血者乙型肝炎表面抗原(HBsAg)及抗-丙型肝炎病毒(HCV)ELISA筛查不合格标本的假阳性情况.方法 对2009年2~5月常规国产和进口双试剂ELISA筛查HBsAg不合格的107份标本采用核酸和血清学中和试验加以确认,对常规国产和进口双试剂ELISA筛查抗-HCV不合格的184份标本采用核酸和血清学RIBA试验加以确认.核酸和(或)血清学补充试验阳性判为确认阳性,不能被确认阳性者判为假阳性.对假阳性情况进行统计分析.结果 HBsAg筛查不合格标本中,HBsAg ELISA双试剂阳性、单试剂阳性、灰区标本的假阳性率分别为2.0%、58.7%、63.6%,总假阳性率为32.7%;抗-HCV筛查不合格标本中,抗-HCV ELISA试剂假阳性率分别为23.9% 、95.2%、96.1%,总假阳性率为67.9%.HBsAg国产试剂单阳及灰区的假阳性率[78.6%(11/14),100%(3/3)]高于进口试剂单阳及灰区的假阳性率[50.0%(16/32),50.0% (4/8);x2=5.188,P<0.05];抗-HCV国产试剂单阳及灰区的假阳性率[96.3% (26/27),95.5% (21/22)]与进口试剂单阳及灰区的假阳性率[94.3% (33/35),93.5%(29/31)]差别不大(x2=1.048,P>0.05).结论 灰区标本的假阳性率极高,但从血液安全考虑灰区设置有必要;抗-HCV ELISA检测的假阳性问题较HBsAg ELISA严重;针对血液筛查假阳性问题,建议对血液及献血者应独立管理,建立献血者归队方案.目前HBsAg进口试剂的特异性优于国产试剂,而抗-HCV试剂的特异性进口试剂与国产试剂差别不大.

  19. Immune effect of different hepatitis A vaccine and emergency vaccination of appropriate technology study(1)Analysis on the results of anti-HAV,HBsAg,and ALT of 829 children at age of 4 to 10 in Huadian city%不同甲型肝炎疫苗免疫效果和应急接种适宜技术研究(一)桦甸市829名4~10岁儿童抗HAV、HBsAg和ALT检测结果分析

    Institute of Scientific and Technical Information of China (English)

    王洪海; 范学彬; 姜桂秋; 王艳萍; 刘娟; 陈超; 周剑惠; 曹风瑞; 潘飞

    2011-01-01

    目的 对桦甸市829名儿童的抗HAV、HBsAg和ALT检测结果进行分析,从而确定健康人群.方法 采用整群随机抽样的方法,对1~3年级学生和学前班儿童进行体检和问卷调查,选择既往无甲肝病史和甲肝疫苗接种史的人群作为研究对象,检测其血液中抗HAV、HBsAg和ALT的情况.结果 通过对829名学生进行检测,3项均为正常者为710名.结论 通过检测,确定了健康人群,为评价疫苗的安全性、免疫原性,比较儿童接种不同类型甲肝疫苗后不同时间抗体水平变化,以及应急接种方案确定了研究对象.%Objective To analysis the testing results of anti - HAV, HBsAg and ALT of 829 children in Huadian city, consequently, made sure the healthy population. Method Adopting randomly chester sampling method, students of grade 1 to grade 3 and preschool children had physical examination and questionnaires, selecting the people with no previous hepatitis A history and no hepatitis A vaccination history as research objects, testing anti - HAV, HBsAg and ALT in their blood. Results Examined 829 children, in them, 710 were normal. Conclusions By detection, we made sure the healthy population, and provided research objects for evaluation vaccine safety, immunogenicity, compared antibody levels of children at different time of different hepatitis A vaccination types, and emergency vaccination program

  20. 乙肝病毒表面抗原和抗体双阳性者中病毒S区基因序列分析%The Sequence Analysis of HBV S Gene Isolated from Both HBsAg and Anti-HBs Antibody Positive Blood Donors

    Institute of Scientific and Technical Information of China (English)

    辜文洁; 林京香; 王佑春; 张华远; 蓝海云; 李河民

    2001-01-01

    为了解乙肝病毒(HBV)表面抗原和抗体双阳性患者中病毒的基因型及其HVB S区是否有变异.用放射免疫试剂检测HBsAg阳性样品中的抗-HBs抗体,用聚合酶链反应法检测双阳性样品中的HBV DNA,然后对阳性样品进行克隆和基因序列分析,并将所得序列与HBV不同基因型的代表株进行比较分析.结果显示389例HBsAg阳性样品中有10例为抗HBs抗体阳性;该10例双阳性样品中有5例为HBV DNA阳性;序列分析显示该5株HBV均为B基因型,其中4株为adw亚型,1株为adr亚型;其中有2株在S区的"a"决定簇的氨基酸发生了变异.

  1. The Lumipulse G HBsAg-Quant assay for screening and quantification of the hepatitis B surface antigen.

    Science.gov (United States)

    Yang, Ruifeng; Song, Guangjun; Guan, Wenli; Wang, Qian; Liu, Yan; Wei, Lai

    2016-02-01

    Qualitative HBsAg assay is used to screen HBV infection for decades. The utility of quantitative assay is also rejuvenated recently. We aimed to evaluate and compare the performance of a novel ultra-sensitive and quantitative assay, the Lumipulse assay, with the Architect and Elecsys assays. As screening methods, specificity was compared using 2043 consecutive clinical routine samples. As quantitative assays, precision and accuracy were assessed. Sera from 112 treatment-naïve chronic hepatitis B patients, four patients undergoing antiviral therapy and one patient with acute infection were tested to compare the correlations. Samples with concurrent HBsAg/anti-HBs were also quantified. The Lumipulse assay precisely quantified ultra-low level of HBsAg (0.004 IU/mL). It identified additional 0.98% (20/2043) clinical samples with trance amount of HBsAg. Three assays displayed excellent linear correlations irrespective of genotypes and S-gene mutations (R(2)>0.95, PLumipulse assay did not yield higher HBsAg concentrations in samples with concomitant anti-HBs. Compared with other assays, the Lumipulse assay is sensitive and specific for detecting HBsAg. The interpretation of the extremely low-level results, however, is challenging. Quantitative HBsAg results by different assays are highly correlated, but they should be interpreted interchangeably only after conversion to eliminate the biases.

  2. Obtención de un conjugado anti IgG de ratón - FITC mediante la tecnología IgY para uso como anticuerpo secundario en la detección de antígenos de superficie celular

    Directory of Open Access Journals (Sweden)

    Esteban J. Gutiérrez Calzado

    2007-01-01

    Full Text Available Desde el año 1996, el Centro Europeo para la Validación de Métodos Alternativos recomendó el uso de la IgY como sustituto de la IgG de mamíferos con el objetivo de reducir al mínimo las situaciones de daño que se les causa a los animales que se someten a la producción de anticuerpos (Acs. En 1999, la tecnología IgY se aprobó por la Oficina Federal Veterinaria del gobierno suizo como un método alternativo para sustentar el cuidado y bienestar de los animales. Muchas de las publicaciones relacionadas con IgY demuestran las características desde el punto de vista bioquímico de esta inmunoglobulina que la hace tomar ventajas sobre la IgG, entre las que refiere que los complejos inmunes que contienen Acs aviares, no pueden interactuar con los receptores Fc o del complemento sobre las células, por lo que el reemplazamiento de los Acs de mamíferos por sus similares en gallinas pudieran evitar interferencias en muchos ensayos diagnósticos. Este conocimiento ha sido la base objetiva de este trabajo que consistió en la obtención de Acs IgY anti IgG de ratón con elevado grado de pureza, los que fueron conjugados por unión covalente vía grupos amino primarios libres al isotiocianato de fluoresceína (FITC. Estos conjugados mostraron poseer una razón molar FITC/proteína en intervalos reportados para un buen desempeño. La dilución óptima de trabajo obtenida para ellos fue muy adecuada y demostró su utilidad como Acs secundarios en el conteo de antígenos de superficie celular linfocitario humano por sus resultados comparables con los reactivos similares de origen mamífero ensayados en paralelo.

  3. Distribución heterogénea de la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre en Puebla, México Heterogeneous distribution of the prevalence of anti-Trypanosoma cruzi antibodies among blood donors in the State of Puebla, Mexico

    Directory of Open Access Journals (Sweden)

    Víctor M Monteón

    2005-04-01

    Full Text Available OBJETIVO: Identificar la seroprevalencia de anticuerpos anti Trypanosoma cruzi (Ac anti-T. cruzi en donadores de sangre que habitan en ámbito rural y suburbano, así como las regiones del estado de mayor riesgo y factores asociados. MATERIAL Y MÉTODOS: Estudio transversal realizado de enero a diciembre de 2003. Se analizaron 2 489 donadores de sangre reclutados en 10 puestos de sangrado del Instituto Mexicano del Seguro Social (IMSS distribuidos en las siete regiones económicas del estado de Puebla, México. Se determinó la seroprevalencia mediante las pruebas serológicas obligatorias del panel viral y, además, para T. cruzi, región de reclutamiento y de origen de los donadores. RESULTADOS: La seroprevalencia de Ac anti-T. cruzi fue de 1.24% (31/2 489 comparable con la obtenida para el virus de la hepatitis C (1.5% y por arriba de la del virus de la inmunodeficiencia humana (0.4% y del antígeno de superficie del virus de la hepatitis B (0.3%. Las regiones de Tehuacán-Sierra Negra y Mixteca fueron las de mayor riesgo con seroprevalencias, por el origen del donador, de 2.6% para T. cruzi, mientras que en los originarios de las regiones Sierra nororiental y Angelópolis no se detectaron casos positivos. Se observó asociación entre ser seropositivo y mayor de 40 años y ser originario de las regiones de Tehuacán-Sierra Negra y Mixteca. CONCLUSIONES: La distribución de seroprevalencia a T. cruzi es heterogénea, oscila desde 0% hasta 2.6%, y se reconoce a Tehuacán-Sierra Negra y Mixteca como las regiones de mayor riesgo.OBJECTIVE: To determine the seroprevalence and associated factors, of antibodies against Trypanosoma cruzi (T. cruzi Ab among blood donors living in rural and suburban areas and risk regions. MATERIAL AND METHODS: A cross-sectional study was conducted from January to December 2003, in 2489 blood donors of seven regions of Puebla, who were evaluated for mandatory viral and T. cruzi serological tests using validated

  4. Anti-HBs levels in infants of hepatitis B carrier mothers after delayed active immunization with recombinant vaccine concomitant with DTP-polio vaccine: Is there need for a second dose of HBIg?

    OpenAIRE

    Grosheide, Pia Maria; Del Canho, R.; Voogd, M.; Heijtink, R.A.; Schalm, Solko

    1994-01-01

    textabstractThe need for an additional dose of hepatitis B immune globulin (HBIg) was studied by comparing infants receiving 1 ml HBIg at birth followed by hepatitis B immunization, concomitant with DTP-polio vaccine, at 3, 4, 5 and 11 months (schedule E), with infants receiving the same schedule with additional HBIg at 3 months (schedule F). The immune response to recombinant hepatitis B vaccine (20 μg) was evaluated in 195 infants born to HBsAg-positive mothers allocated to groups E and F a...

  5. A novel stop codon mutation in HBsAg gene identified in a hepatitis B virus strain associated with cryptogenic cirrhosis

    Institute of Scientific and Technical Information of China (English)

    Xu Yang; Xiao-Peng Tang; Jian-Hua Lei; Hong-Yu Luo; Yong-Hong Zhang

    2003-01-01

    AIM: HBsAg is the most important serological marker for acute or chronic hepatitis B. Nevertheless, there were reports of HBsAg-negative infection caused by hepatitis B virus in recent years. We had a patient with crytogenic cirrhosis who was negative for HBsAg, positive for anti-HBs and HBeAg.This paper was to explore the pathogenic and molecular basis of the unusual serological pattern.METHODS: HBV serologic markers were qualitatively and quantitatively determined. HBV DNA in serum was qualitatively tested using routine Polymerase chain reaction (PCR), and the viral level was determined with real-time fluorescence quantitative PCR. HBsAg gene was amplified and cloned. Four clones were sequenced. The new genomic sequences were compared with GenBank on the DNA level as well as the protein level.RESULTS: The qualitative results of serological markers were HBsAg(-), anti-HBs(+), HBeAg(+), anti-HBe(-) and anti-HBc(+). The quantitative results of serological marker were HBsAg (S/N): 0.77 (cut off of S/N: ≥2.00), HBeAg (S/N): 56.43 (cut off S/N: ≥2.10), anti-HBc (S/Co): 2.03 (cut off of S/Co: ≤ 1.00). The viral level was as high as 1.54×109copies/ml. Sequencing of the HBsAg gene clones revealed a unique point mutation at nucleotide 336 (C to A), which resulted in a novel stop codon at aa 61. The novel HBsAg gene stop mutation had not been described.CONCLUSION: The lack of detection of HBsAg in the presence of high viral levels of replication may be caused by the existence of viral genomes harboring point mutations which resulted in stop codon upstream of the″a″ determinant in HBsAg gene.

  6. Prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre del IMSS, Orizaba, Veracruz, México

    OpenAIRE

    Ramos-Ligonio Angel; Ramírez-Sánchez Michaía Elián; González-Hernández Juan Carlos; Rosales-Encina José Luis; López-Monteon Aracely

    2006-01-01

    OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS) en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO) del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70) y un extracto crudo de epimastigotes. Las muestras fueron o...

  7. Avaliação eosinofílica e soropositividade para anticorpos IgG anti-toxocara em crianças atendidas pelo Sistema Único de Saúde Evaluación eosinófila y suero-positividad para anticuerpos igG anti-toxocara en niños atendidos por el Sistema Único de Salud Eosinophilic count and seropositivity for IgG antibodies to toxocara spp. in children assisted at the public health service

    Directory of Open Access Journals (Sweden)

    Ariella Andrade Marchioro

    2011-03-01

    Full Text Available OBJETIVO: Verificar a associação entre a presença de eosinofilia e a soropositividade para anticorpos IgG anti-Toxocara spp. em crianças atendidas pelo Sistema Único de Saúde no Noroeste do Paraná, Brasil. MÉTODOS: Estudo retrospectivo com crianças de sete meses a 12 anos, atendidas pelo Sistema Único de Saúde do Noroeste do Paraná, com teste ELISA para a pesquisa de anticorpos IgG anti-Toxocara spp. e contagem de eosinófilos (eosinofilia >600 células/mm³. RESULTADOS: Entre as 1.199 crianças, 386 (32,2% apresentaram anticorpos IgG anti-Toxocara spp. A soroprevalência e a eosinofilia foram mais frequentes em crianças de sete meses a cinco anos. A eosinofilia foi observada em 7,8% dos pacientes soro-reagentes ao Toxocara spp.. CONCLUSÕES: Foi observada elevada prevalência de anticorpos anti-Toxocara spp., principalmente nos menores de cinco anos. Com exceção de algumas crianças que apresentaram sintomas respiratórios e presença de eosinofilia, a maioria foi assintomática e não mostrava eosinofilia. A pesquisa de eosinófilos é ferramenta secundária para o diagnóstico de toxocaríase.OBJETIVO: Verificar la asociación entre la presencia de eosinofilia y una suero-positividad para anticuerpos IgG anti-Toxocara spp. en niños atendidos por el Sistema Único de Salud en el noroeste de Paraná, Brasil. MÉTODOS: Estudio retrospectivo en niños de siete meses a 12 años de edad, atendidos por el Sistema Único de Salud en el noroeste de Paraná, con prueba de ELISA para la investigación de anticuerpos IgG anti-Toxocara spp. y recuento de eosinófilos (eosinofilia > 600 células/mm³. RESULTADOS: Entre los 1.199 niños, 386 (32,2% presentaron anticuerpos IgG anti-Toxocara spp. La suero-prevalencia y la eosinofilia fueron más frecuentes en niños de siete meses a cinco años. La eosinofilia fue observada en 7,8% de los pacientes suero-reactivos al Toxocara spp. CONCLUSIÓN: Se observó elevada prevalencia de anticuerpos

  8. Antisperm autoantibodies and infertility Anticuerpos antiespermatozoides e infertilidad

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    Jorge Eliécer Ossa Londoño

    1997-02-01

    Full Text Available Fourteen human infertile couples were studied along with seven fertile control couples in order to determine the presence of antisperm au. toantibodies and to correlate results with their clinical reproductive status, spermogram parameters and the quality of cervical mucus. Immunoglobulins of IgG and IgA isotypes were evaluated on spermatozoa by the direct immunobead technique and in serum and cervical mucus by the indirect method. The following differences were found: Nine of 14 male patients but only 1 of 7 male controls had IgG on spermatozoa (p= 0.042 and OR= 10.8. Seven out of 14 male patiens and 1 of 7 controls were positive for IgA on spermatozoa (p= 0.12. Infertile males had a higher frequency of abnormal spermogram parameters as compared to controls (p= 0.0029 and OR= 3.32. Interestingly, the presence of only IgG in serum appeared to be a "protective factor" against IgG on sperm; only one out of the 16 positive males contradicted this observation. Para determinar la presencia de anticuerpos antiespermatozoides y correlacionarla con la situación reproductiva, el espermograma y la calidad del moco cervical, se estudiaron 7 parejas sanas, con hijos, y 14 infértiles. Se determinaron por las técnicas directa e indirecta de inmunoesferas los anticuerpos de los isotipos IgG e IgA en espermatozoides, suero y moco cervical. En 9 de los 14 hombres infértiles y en 1 de los 7 fértiles se encontraron anticuerpos IgG en la superficie de los espermatozoides (p= 0.042 y una Razón de disparidad [RD] = 10.8 y 8 de éstos también tuvieron IgA en los espermatozoides. El número de pruebas alteradas en el espermograma de los hombres infértiles fue significativamente mayor (p= 0.0029 y RD = 3.32. Tanto en los hombres infértiles como en los fértiles la presencia de sólo IgG en el suero parece ser un "factor de protección" contra IgG en los espermatozoides; de los 16 hombres que presentaron anticuerpos, fuera en el suero o en los espermatozoides, s

  9. Enhancement of humoral immune responses to HBsAg by heat shock protein gp96 and its N-terminal fragment in mice

    Institute of Scientific and Technical Information of China (English)

    Hong-Tao Li; Jia-Bin Yan; Jing Li; Ming-Hai Zhou; Xiao-Dong Zhu; Yu-Xia Zhang; Po Tien

    2005-01-01

    AIM: Most studies on the immune effect of gp96 were focused on its enhancement of CTLs. It is interesting to know whether gp96 could influence the humoral immune response, and whether the recombinant N-terminal fragment of gp96 could substitute native gp96 to stimulate the immune system.METHODS: gp96 isolated from livers of normal mice and its N-terminal fragment (amino acid 22-355) expressed in E coli were used for immunization of BALb/c mice. Eight groups of mice received one of the following regiments subcutaneously in 100 μL phosphate buffered saline (PBS)at an interval of 3 wk. Group 1: PBS only; group 2:gp96 only; group 3: N-terminal fragment only; group 4: HBsAg only; group 5: HBsAg+gp96; group 6: HBsAg+N-terminalfragment; group 7: HBsAg+incomplete Freud's adjuvant; group 8: HBsAg+N-terminal fragment (95 ℃ heated for 30 min). Serum anti-HBsAg antibody levels were assayed by ELISA. CTL responses in splenocytes were analyzed by ELISPOT after the last vaccination.RESULTS: The average titer of serum anti-HBsAg antibodyin the mice immunized with HBsAg together with gp96 or its N-terminal fragment were much higher than those immunized with HBsAg alone detected by ELISA. The cellular immune response of the mice immunized with HBsAg together with gp96 or its N-terminal fragment was not different with those immunized with HBsAg alone measured by ELISPOT assay.CONCLUSION: gp96 or its N-terminal fragment greatly improved humoral immune response induced by HBsAg, but failed to enhance the CTL response, which demonstrated the potential of using gp96 or its N-terminal fragment as a possible adjuvant to augment humoral immune response against HBV infection.

  10. Frecuencia de anticuerpos contra el virus C de la hepatitis en pacientes con cirrosis hepática en Yucatán, México Frequency of antibodies against the hepatitis C virus in patients with hepatic cirrhosis in Yucatan, Mexico

    Directory of Open Access Journals (Sweden)

    Renán A Góngora-Biachi

    2003-10-01

    Full Text Available OBJETIVO: En este estudio reportamos la prevalencia de anticuerpos contra el virus C de la hepatitis (Ac-VCH en un grupo de pacientes con cirrosis hepática (CH. MATERIAL Y MÉTODOS: Se hizo un estudio prospectivo, transversal y descriptivo, de marzo de 1998 a mayo de 1999. Se estudiaron a 153 pacientes (117 (76% hombres y 36 (24% mujeres con diagnóstico de CH, que eran atendidos en el Hospital General Agustín O' Horan y en el Centro de Investigaciones Regionales Doctor Hideyo Noguchi, en la ciudad de Mérida, Yucatán, México. Se aplicó un cuestionario con datos clínico-epidemiológicos y se determinó la presencia de Ac-VCH (ELISA de 2ª generación y RIBA-2 para confirmar el diagnóstico a cada paciente. Se determinó también el antígeno de superficie de la hepatitis B (AgsHB y anticuerpos contra el antígeno central de la hepatitis B (Anti-HBc mediante el método de ELISA. La presencia de Ac-VCH fue relacionada con las variables epidemiológicas de los sujetos. La prevalencia de anti-HCV y la frecuencia de características se compararon entre los pacientes positivos y negativos con las pruebas de c² y exacta de Fisher. RESULTADOS: El 32% de los pacientes con CH (35/117 (30% hombres y 14/36 (39% mujeres fueron positivos para los Ac-VCH. El alcoholismo estuvo presente en todos los hombres serorreactivos y en ninguna de las mujeres positivas (pOBJECTIVE: To report the prevalence of antibodies against the hepatitis C virus (anti-HCV in a group of patients with hepatic cirrhosis (HC. MATERIAL AND METHODS: A prospective transversal and descriptive study was carried out from March 1998 to May 1999. Study subjects were 153 patients; 117 (76% male and 36 (24% female, diagnosed with HC. They were attended at the General Hospital Agustín O' Horan and at Regional Research Center Doctor Hideyo Noguchi, in Merida, Yucatan, Mexico. A clinical-epidemiologic questionnaire completed by interview was used for data collection. Anti-HCV were detected

  11. HDV Seroprevalence in HBsAg Positive Patients

    International Nuclear Information System (INIS)

    Objective: To find out the frequency of HDV seroprevalence and the demographic characteristics or HBsAg-HDV positive patients. Study Design: Cross-sectional analytical study. Place and Duration of Study: Jinnah Postgraduate Medical Centre and Civil Hospital, Medical Unit-III, Karachi, from March 2007 to April 2011. Methodology: Patients with positive HBsAg were included in the study. Those having co-infection with HCV or HIV, autoimmune hepatitis, alcoholic hepatitis, Wilson's disease and haemochromatosis were excluded. After detailed history and physical examination all the patients underwent laboratory workup including complete blood count, liver function test, viral profile (HAV, HCV, HIV and anti-HDV) and prothrombin time. While in selected patients, HBc (core) antibodies, ultrasound abdomen, serum iron profile, ANA and liver biopsy were also carried out whenever needed to establish a clinical stage of liver disease. Results: There were 374 patients with 266 (71.1%) males and 108 (28.9%) females with overall mean age of 31.64 +- 8.66 years. Overall frequency of anti-HDV antibodies positivity was found in 28.1% (n = 105) patients. HDV seropositivity was slightly more prevalent in males as compared to females (28.57% vs. 26.57%). HDV seropositivity frequency was significantly higher in patients who presented with acute hepatitis/hepatic failure as compared to other clinical diagnoses (p = 0.027) and in those sub-sets of patients who had raised ALT levels (p = 0.012). Conclusion: There was a high frequency of HDV seropositivity in the studied population particularly in males with acute hepatitis or hepatic failure, having raised ALT levels. The emphasis should be on preventive measures taken by other countries to reduce the prevalence of these treatment challenging infections. (author)

  12. Detección de pepsinógeno mediante el uso de antisueros y anticuerpos monoclonales.

    Directory of Open Access Journals (Sweden)

    Oscar Orozco

    2000-02-01

    Full Text Available

    El pepsinógeno (PGA y PGC es una aspartato proteasa secretada por células principales gástricas que a pH ácido se convierte en pepsina. Los niveles séricos de dicha enzima se han postulado como indicadores del estado de la mucosa gástrica ya que dependiendo de la patología de base estos pueden encontrarse elevados o disminuidos. Así, en la gastritis superficial y úlcera duodenal los niveles de PGA se encuentran aumentados, mientras que en la gastritis atrófica y cáncer gástrico se encuentran disminuidos. Se planteó como objetivo de este trabajo producir anticuerpos policlonales y monoclonales anti-pepsinógeno con el fin de ser empleados en el diseño de sistemas de cuantificación de pepsinógeno sérico, ya que los sistemas de cuantificación comerciales son muy costosos y de difícil acceso. Para la producción de los anticuerpos se empleó pepsina A porcina como inmunógeno teniendo en cuenta que presenta una alta homología en la secuencia de aminoácidos (86% con la pepsina A humana y además tienen una estructura secundaria idéntica. Anticuerpos policlonales fueron obtenidos de conejos Nueva Zelanda inmunizados vía subcutánea con pepsina A porcina y los anticuerpos monoclonales a partir de células de bazo de los ratones BALB/c inmunizados con pepsina A vía intraperitoneal. Los anticuerpos tanto policlonales como monoclonales fueron purificados por precipitación con sulfato de amonio y posteriormente cromatografía de intercambio iónico a partir del suero o líquido ascítico respectivamente. Los anticuerpos purificados, tanto policlonales como monoclonales, son capaces de reconocer pepsina porcina y pepsinógeno humano presente en sonicados de biopsias gástricas en ensayos de ELISA y western blot. En ensayos de inmunohistoquímica realizados sobre cortes de biopsias gástricas los anticuerpos monoclonales detectan el pepsinógeno presente en el

  13. Improved cell mediated immune responses after successful re-vaccination of non-responders to the hepatitis B virus surface antigen (HBsAg) vaccine using the combined hepatitis A and B vaccine.

    Science.gov (United States)

    Nyström, Jessica; Cardell, Kristina; Björnsdottir, Thora Björg; Fryden, Aril; Hultgren, Catharina; Sällberg, Matti

    2008-11-01

    We successfully re-vaccinated hepatitis B virus (HBV) vaccine non-responders using a double dose of the combined hepatitis A virus (HAV) and HBV vaccine. The hope was to improve priming of hepatitis B surface antigen (HBsAg)-specific cell mediated immune response (CMI) by an increased antigen dose and a theoretical adjuvant-effect from the local presence of a HAV-specific CMI. A few non-responders had a detectable HBsAg-specific CMI before re-vaccination. An in vitro detectable HBsAg-specific CMI was primed equally effective in non-responders (58%) as in first time vaccine recipients (68%). After the third dose a weak, albeit significant, association was observed between the magnitude of HBsAg-specific proliferation and anti-HBs levels. This regimen improves the priming of HBsAg-specific CMIs and antibodies.

  14. HBsAg inhibits the translocation of JTB into mitochondria in HepG2 cells and potentially plays a role in HCC progression.

    Directory of Open Access Journals (Sweden)

    Yun-Peng Liu

    Full Text Available BACKGROUND AND AIMS: The expression of the jumping translocation breakpoint (JTB gene is upregulated in malignant liver tissues; however, JTB is associated with unbalanced translocations in many other types of cancer that suppress JTB expression. No comprehensive analysis on its function in human hepatocellular carcinoma (HCC has been performed to date. We aimed to define the biological consequences for interaction between JTB and HBsAg in HCC cell lines. METHODS: We employed the stable transfection to establish small HBsAg expressing HepG2 cell line, and stably silenced the JTB expression using short hairpin RNA in HepG2 cell line. The effects of JTB and small HBsAg in vitro were determined by assessing cell apoptosis and motility. RESULTS: Silencing of JTB expression promoted cancer cell motility and reduced cell apoptosis, which was significantly enhanced by HBs expression. Expression of HBsAg inhibited the translocation of JTB to the mitochondria. Furthermore, silencing of the JTB resulted in an increase in the phosphorylation of p65 in HepG2 cells and HepG2-HBs cells, whereas HBsAg expression decreased the phosphorylation of p65. The silencing of JTB in HepG2-HBs cells conferred increased advantages in cell motility and anti-apoptosis. CONCLUSION: HBsAg inhibited the translocation of JTB to the mitochondria and decreased the phosphorylation of p65 through the interaction with JTB, After JTB knockdown, HBsAg exhibited a stronger potential to promote tumor progression. Our data suggested that JTB act as a tumor suppressor gene in regards to HBV infection and its activation might be applied as a therapeutic strategy for in control of HBV related HCC development.

  15. Observation on immune effect of hepatitis B vaccine in infants born to HBsAg positive mothers%HBsAg阳性母亲的婴儿乙肝疫苗免疫效果观察

    Institute of Scientific and Technical Information of China (English)

    丰淑英

    2011-01-01

    目的 比较HBsAg阳性母亲所生婴儿接种不同剂量乙肝疫苗后的免疫效果.方法 将HBsAg阳性母亲所生婴儿118例根据知情、自愿原则分为两组,每组59例.分别接种10 μg重组酵母乙肝疫苗和20 μg重组CHO(中国仓鼠卵巢细胞)乙肝疫苗,检测婴儿出生和12月龄时HBsAg和抗-HBs水平,比较不同剂量疫苗免疫后阻断乙肝病毒母婴传播效果.结果 婴儿出生时,接种10 μg和20 μg乙肝疫苗组婴儿HBsAg阳性率分别为8.47%和5.08%,差异无统计学意义.12月龄随访时,接种10 μg和20 μg乙肝疫苗的婴儿HBsAg阳性率分别为3.4%和1.7%,抗-HBs阳性率分别为83.1%和88.1%,差异无统计学意义;10 μg乙肝疫苗组婴儿抗-HBs无/弱应答率高于20 μg组婴儿,差异具有统计学意义(52.5% vs 33.9%,P<0.05).结论 20 μg乙肝疫苗免疫效果优于10 μg乙肝疫苗,对于母亲HBsAg阳性的婴儿,建议接种20 μg乙肝疫苗.%Objective To investigate the immune effect of different dosages of hepatitis B( HB ) vaccine in infants born to HBsAg positive mothers. Methods A total of 118 infants born to HBsAg positive mothers were divided into two groups,and were immunized with recombinant yeast-derived hepatitis B vaccines( 10 μg )or recombinant CHO cell derived hepatitis B vaccines( 20 g ), respectively. The HBsAg and anti-HBs were detected. The blocking effects of different doses of vaccine on mother-to-infant transmission of hepatitis B virus were compared. Results When the infants were born,HBsAg positive rate were 8.47% and 5.08% in 10 μg group and 20 μg group, respectively, and there was no significant difference between 2 groups. When the infants were 12 months old, HBsAg and antiHBs positive rates were not statistically significant between 2 groups, but the non( low )-responsive rate in 10 μg group was significantly higher than that in 20 μg group( P < 0.05 ). Conclusion The immune effect of 20 μg vaccine is better than that of 10 μg vaccine

  16.  Active-passive Immunization Effectiveness Against Hepatitis B Virus in Children Born to HBsAg Positive Mothers in Amol, North of Iran

    Directory of Open Access Journals (Sweden)

    2011-11-01

    Full Text Available  Objectives: HBV infection is a contagious disease that may transmit vertically from mothers to their neonates or horizontally by blood products and body secretions. Over 50�0of Iranian carriers have contracted the infection perinatally, making this the most likely route of transmission of HBV in Iran. This study assesses the serologic markers of HBV in children born to HBsAg positive mothers who received HBIG and 3 doses of HBV vaccine. Methods: To evaluate the effectiveness of vaccination against HBV, a study was conducted on 95 Children, born to hepatitis B surface antigen (HBsAg-positive mothers, who had received Hepatitis B Immune Globulin and HBV vaccines during 2004-2008. All children were tested for the presence of HBsAg, anti-HBs and anti-HB core antigen (anti-HBc. Results: Among an estimated 30000 pregnant women during the five year study, about 130 (0.42�20were HBV carriers. Ninety-five children from these mothers were enrolled in this study. Only one child (1.1�20was HBsAg positive, while 88.4�0of children were Anti-HBs Positive. Eleven children (11.6�20were exposed to HBV as shown by the presence of anti-HBc. A significant difference was observed between the children’s age and Anti-HBs (p=0.0001. Conclusion: Passive-active immunoprophylaxis of high risk babies was highly efficacious in preventing perinatal transmission of the HBV carrier state. Also, evaluation of serologic markers in HBV infected people is important for designing the strategies for disease control.

  17. Prevalence of Hepatitis B surface antigen (HBsAg among visitors of Shashemene General Hospital voluntary counseling and testing center

    Directory of Open Access Journals (Sweden)

    Medhin Girmay

    2011-02-01

    Full Text Available Abstract Background Hepatitis B virus (HBV infection is significant health problem, as it can lead to chronic hepatitis, liver cirrhosis, and hepatic carcinoma. Due to shared routes of transmission, HBV and human immunodeficiency virus (HIV co-infection is common and is an emerging concern in the clinical management of patients because of increased mortality, accelerated hepatic disease progression, and the frequent hepatotoxicity caused by anti-retroviral therapy. The aim of this study was to determine the prevalence of Hepatitis B surface antigen (HBsAg and its risk factors, among individuals visiting Shashemene General Hospital VCT center. Findings Institution based cross-sectional study was performed from November 3, 2008 to December 29, 2008 and 384 voluntary counseling and testing (VCT clients were investigated. Data on socio demographic and HBV risk factors was collected using structured questionnaires. Blood samples were collected and screened for hepatitis B surface antigen (HBsAg and HIV by commercially available rapid test kits. The prevalence of HBsAg in this study group was 5.7%. Fourteen percent of HIV positive subjects (8/57 and 4.3% (14/327 of HIV negative subjects were positive for HBsAg. Significantly high prevalence of HBsAg was observed among individuals who had history of invasive procedures, like tooth extraction, abortion and ear piercing; history of hospital admission, history of unsafe inject and HIV positives. Conclusions Although HBsAg prevalence is much higher among subjects who are HIV positive (14.0% versus 4.3%, the prevalence of HBsAg in HIV negative subjects is high enough to warrant a recommendation to screen all clients at VCT centers irrespective of HIV status.

  18. Quantification of HBsAg: Basic virology for clinical practice

    Institute of Scientific and Technical Information of China (English)

    Jung Min Lee; Sang Hoon Ahn

    2011-01-01

    Hepatitis B surface antigen (HBsAg) is produced and secreted through a complex mechanism that is still not fully understood. In clinical fields, HBsAg has longserved as a qualitative diagnostic marker for hepatitis B virus infection. Notably, advances have been made in the development of quantitative HBsAg assays, whichhave allowed viral replication monitoring, and there is an opportunity to make maximal use of quantitative HBsAg to elucidate its role in clinical fields. Yet, it needs to be underscored that a further understanding of HBsAg, not only from clinical point of view but also from a virologic point of view, would enable us to deepen our insights, so that we could more widely expand and applyits utility. It is also important to be familiar with HBsAg variants and their clinical consequences in terms of immune escape mutants, issues resulting from overlap with corresponding mutation in the P gene, and detection problems for the HBsAg variants. In this article, we review current concepts and issues on the quantificationof HBsAg titers with respect to their biologic nature, method principles, and clinically relevant topics.

  19. Viral hepatitis and rapid diagnostic test based screening for HBsAg in HIV-infected patients in rural Tanzania.

    Directory of Open Access Journals (Sweden)

    Fabian C Franzeck

    Full Text Available BACKGROUND: Co-infection with hepatitis B virus (HBV is highly prevalent in people living with HIV in Sub-Saharan Africa. Screening for HBV surface antigen (HBsAg before initiation of combination antiretroviral therapy (cART is recommended. However, it is not part of diagnostic routines in HIV programs in many resource-limited countries although patients could benefit from optimized antiretroviral therapy covering both infections. Screening could be facilitated by rapid diagnostic tests for HBsAg. Operating experience with these point of care devices in HIV-positive patients in Sub-Saharan Africa is largely lacking. We determined the prevalence of HBV and Hepatitis C virus (HCV infection as well as the diagnostic accuracy of the rapid test device Determine HBsAg in an HIV cohort in rural Tanzania. METHODS: Prospectively collected blood samples from adult, HIV-1 positive and antiretroviral treatment-naïve patients in the Kilombero and Ulanga antiretroviral cohort (KIULARCO in rural Tanzania were analyzed at the point of care with Determine HBsAg, a reference HBsAg EIA and an anti-HCV EIA. RESULTS: Samples of 272 patients were included. Median age was 38 years (interquartile range [IQR] 32-47, 169/272 (63% subjects were females and median CD4+ count was 250 cells/µL (IQR 97-439. HBsAg was detected in 25/272 (9.2%, 95% confidence interval [CI] 6.2-13.0% subjects. Of these, 7/25 (28% were positive for HBeAg. Sensitivity of Determine HBsAg was rated at 96% (95% CI 82.8-99.6% and specificity at 100% (95% CI, 98.9-100%. Antibodies to HCV (anti-HCV were found in 10/272 (3.7%, 95% CI 2.0-6.4% of patients. CONCLUSION: This study reports a high prevalence of HBV in HIV-positive patients in a rural Tanzanian setting. The rapid diagnostic test Determine HBsAg is an accurate assay for screening for HBsAg in HIV-1 infected patients at the point of care and may further help to guide cART in Sub-Saharan Africa.

  20. Preparation and Properties of HBS Lignin from Masson Pine

    Institute of Scientific and Technical Information of China (English)

    CHENG Xian-su; CHEN Wei-jian; CHEN Yun-ping; CHEN Yue-xian; LI Mian-jun; FANG Hua-shu

    2004-01-01

    In order to establish a new method for making cellulose and lignin from Masson pine, a high boiling solvent (HBS) pulping process with an aqueous solvent of 1,4-butanediol was investigated. Masson pine chips were pulped with a 70%-90% aqueous solution of 1,4-butanediol containing a small amount of a catalyst at 200-220 ℃ for 60-180 min. HBS Masson pine cellulose is suitable for making paper. Water-insoluble HBS lignin was separated from the liquor reaction mixture by water precipitation. The recovered high boiling solvent (RHBS) is able to be recycled as a pulping solvent, indicating that the HBS method is a pulping process of Masson pine which is energy saving, resources saving and pollution free. HBS lignin has a better chemical reactivity and a lower ash content than lignin sulfonate.

  1. Hepatitis B virus markers in anti-HBc only positive individuals.

    Science.gov (United States)

    Weber, B; Melchior, W; Gehrke, R; Doerr, H W; Berger, A; Rabenau, H

    2001-07-01

    Isolated reactivity to hepatitis B virus (HBV) core antigen (anti-HBc) is observed relatively frequently in immunocompromised individuals, intravenous drug abusers (IVDA), and in the presence of HCV infection. The reason for the lack of HBsAg is not clear. The aim of the present study was to investigate which factors (genetic variability of S gene, low-level HBsAg, and immune complexes may be responsible for the failure of HBsAg detection with commercial HBsAg screening assays. Dilution series of two recombinant HBsAg escape mutants and dilutions of serum samples from chronic HBV carriers with multiple insertions in the a determinant and different HBsAg subtypes were tested with a highly sensitive assay that detects wild-type HBsAg (Elecsys HBsAg, Roche Diagnostics, Penzberg, Germany) and two assays that detect HBV wild-type and escape mutants (Murex HBsAg Version 3, Murex and Enzygnost HBsAg 5.0, Dade Behring, Marburg, Germany). Elecsys HBsAg showed in comparison to Murex HBsAg Version 3 and Enzygnost HBsAg 5.0 a reduced sensitivity for escape mutant detection. On the other hand, the best performance for HBsAg subtype detection was obtained with Elecsys HBsAg. In the second part of the study, a selected panel of isolated anti-HBc reactive (n = 104) serum samples (AxSYM Core) was submitted to testing by Elecsys HBsAg, Murex HBsAg Version 3, Enzygnost HBsAg 5.0, and HBsAg detection after immune complex dissociation (ICD) and anti-HBs determination with two different assays (AxSYM Ausab and Elecsys Anti-HBs). To assess the specificity of anti-HBc test results, all the samples were tested by a second anti-HBc assay (Elecsys Anti-HBc). Quantitative HBV DNA detection was undertaken with a commercially available HBV PCR assay (Amplicor HBV Monitor). HCV infection was present in 65.4% of anti-HBc only reactive individuals. Five AxSYM Core positive samples were negative by Elecsys Anti-HBc. Overall, 15 (14.4%) AxSYM Ausab negative samples gave positive results with Elecsys

  2. Difference in hTERT Gene Expressions between HbsAg-Positive and HbsAg-Negative Hepatocellular Carcinoma

    Institute of Scientific and Technical Information of China (English)

    GUO Yueqing; ZHOU Xu; LIU Enyu; LI Xingrui; LIU Jinwen; YANG Zhifang; YI Jilin

    2005-01-01

    Summary: To investigate the difference in expression of hTERT gene between HbsAg-positive human hepatocellular carcinoma (HCC) and HbsAg-negative HCC and to explore the relationship between HBV infection and hTERT gene expression in HCC. The expression of hTERT protein in 30 cases of HbsAg positive HCC and 17 cases of HbsAg negative HCC was detected by immunohistochemistry (SP method), and the expression of hTERT mRNA was analyzed by reverse transcription polymerase chain reaction (RT-PCR). t-test, Chi-squared test and cochran- armitage trend test were used to see whether there was an interrelation between HBsAg and hTERT gene in HCC. The expression of hTERT protein was mostly located in plasm and occasionally in the nucleus of liver cancer cells. The positive rate of hTERT protein and hTERT mRNA in HbsAg positive HCC- 93.33 % (28/30) and 83.33 % (25/30) respectively which were much higher than those in HbsAg negative HCC- 52.94 % (9/17), 47.06 % (8/17) (P<0.01) respectively. HbsAg is related to hTERT gene expression in human hepatocellular carcinoma. The hTERT gene activated by the efficacious ingredient of HBV may play an important role in hepatocellular transformation and carcinogenesis.

  3. Efficacy evaluation of two synthetic lysine lipidated tripeptides as vaccine adjuvants against HBsAg.

    Science.gov (United States)

    Sidiq, Tabasum; Khajuria, Anamika; Shafi, Syed; Ismail, Tabasum; Sampath Kumar, Halmathur; Kannappa Srinivas, Vellimedu; Krishna, Ella; Kamal Johri, Rakesh

    2013-04-01

    In the present investigation, adjuvant potential of two novel lipidated tripeptide lysine derivatives (KKSM and KKSMB) was evaluated using various in vitro and animal-derived models of humoral and cell-mediated immune events in response to hepatitis B surface antigen (HBsAg). The results were compared with alum adjuvanted with HBsAg. Both these molecules were found to stimulate anti-HBsAg IgG and neutralizing (IgG1 and IgG2a) antibody titres in mice sera. The two molecules stimulated the proliferation of T-lymphocyte sub-sets (CD4/CD8) as well as the production of soluble mediators of Th1 (IL-2 and IFN-γ) and Th2 response (IL-4) in spleen cell culture supernatant. Furthermore, the two lipidated tripeptides enhanced the CD4, CD8, CD3 and CD19 cell populations as well as CD4/CD8 derived IL-2, IL-4, IFN-γ and TNF-α in whole blood of treated mice. There was found to be the significant enhancement in the release of IL-12, IFN-γ and nitrite content in macrophage supernatant. Moreover, the two lipidated tripeptides enhanced the population of CD80 and CD86 in spleen-derived macrophages and did not show any hemolytic effect on rabbit RBCs. Taken together, these results suggest that both these molecules are the potent enhancers of anti-HBsAg immune response via augmenting Th1/Th2 response in a dose dependent manner. PMID:23474022

  4. Posintro™-HBsAg, a modified ISCOM including HBsAg, induces strong cellular and humoral responses

    DEFF Research Database (Denmark)

    Schiött, Asa; Larsson, Kristina; Manniche, Søren;

    2011-01-01

    To improve the hepatitis B vaccines on the market new adjuvant systems have to substitute aluminium. In this study the hepatitis B surface antigen (HBsAg) was incorporated into a novel adjuvant system, the Posintro™, a modification of the traditional immune stimulatory complexes (ISCOMs). This new...... HBsAg vaccine formulation, Posintro™-HBsAg, was compared to two commercial hepatitis B vaccines including aluminium or monophosphoryl lipid A (MPL) and the two adjuvant systems MF59 and QS21 in their efficiency to prime both cellular and humoral immune responses. The Posintro™-HBsAg induced....... The results demonstrate that this novel experimental vaccine formulation, the Posintro™-HBsAg, is strongly immunogenic and can induce both class I and class II responses in experimental animals. This shows promise both for the protection against hepatitis B virus infection and as a potential therapeutic...

  5. Seguridad post-comercialización de los anticuerpos monoclonales antineoplásicos

    OpenAIRE

    González Iglesias, Verónica

    2015-01-01

    INTRODUCCIÓN: Los primeros anticuerpos monoclonales comercializados en España han sido rituximab, trastuzumab, bevacizumab, cetuximab y panitumumab, en este orden. El estudio de su seguridad en los primeros años postcomercialización es fundamental para definir su relación beneficio/riesgo en las condiciones de uso autorizadas. JUSTIFICACIÓN Y OBJETIVOS: Con el fin de estudiar la seguridad postcomercialización de los anticuerpos monoclonales nos planteamos como objetivos: 1) Analizar las s...

  6. UltramicroELISA indirecto para la deteccion de anticuerpos totales a citomegalovirus en suero humano Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples

    OpenAIRE

    Jose Laferte; Miguel Marrero; Maritza Alvarez; Liliana Jomarron; Serafina Garcia; Susana Vazquez; Luis Morier; Mario Ulacia; Antonio Melchor

    1992-01-01

    Se normalizó un ultramicroELISA indirecto para la detección de anticuerpos a Citomegalovirus (CMV) humano (UMELISA CMV). Se determinó la concentración óptima de antígeno en 30 ug/ml, la dilución de los sueros fue de 1:40 y la dilución de trabajo del conjugado fue de 1:1500. El UMELISA CMV fue comparado con las técnicas de aglutinación de latex para anticuerpos anti-CMV (Dupont de Neumors) y la inmunofluorescencia indirecta (EFT). Los resultados mostraron un alto grado de concordancia y elevad...

  7. [Clinical evaluation of a novel HBsAg quantitative assay].

    Science.gov (United States)

    Takagi, Kazumi; Tanaka, Yasuhito; Naganuma, Hatsue; Hiramatsu, Kumiko; Iida, Takayasu; Takasaka, Yoshimitsu; Mizokami, Masashi

    2007-07-01

    The clinical implication of the hepatitis B surface antigen (HBsAg) concentrations in HBV-infected individuals remains unclear. The aim of this study was to evaluate a novel fully automated Chemiluminescence Enzyme Immunoassay (Sysmex HBsAg quantitative assay) by comparative measurements of the reference serum samples versus two independent commercial assays (Lumipulse f or Architect HBsAg QT). Furthermore, clinical usefulness was assessed for monitoring of the serum HBsAg levels during antiviral therapy. A dilution test using 5 reference-serum samples showed linear correlation curve in range from 0.03 to 2,360 IU/ml. The HBsAg was measured in total of 400 serum samples and 99.8% had consistent results between Sysmex and Lumipulse f. Additionally, a positive linear correlation was observed between Sysmex and Architect. To compare the Architect and Sysmex, both methods were applied to quantify the HBsAg in serum samples with different HBV genotypes/subgenotypes, as well as in serum contained HBV vaccine escape mutants (126S, 145R). Correlation between the methods was observed in results for escape mutants and common genotypes (A, B, C) in Japan. Observed during lamivudine therapy, an increase in HBsAg and HBV DNA concentrations preceded the aminotransferase (ALT) elevation associated with drug-resistant HBV variant emergence (breakthrough hepatitis). In conclusion, reliability of the Sysmex HBsAg quantitative assay was confirmed for all HBV genetic variants common in Japan. Monitoring of serum HBsAg concentrations in addition to HBV DNA quantification, is helpful in evaluation of the response to lamivudine treatment and diagnosis of the breakthrough hepatitis.

  8. Anticuerpos derivados de pacientes se dirigen a células tumorales

    Science.gov (United States)

    Artículo del blog Temas y relatos de un anticuerpo derivado de pacientes que destruye células tumorales en estirpes celulares de varios tipos de cáncer e hizo lento el crecimiento en modelos murinos de cáncer de cerebro y pulmón sin signo de efectos.

  9. Prevalence of antibody to Hepatitis B core antigen and Hepatitis B virus DNA in HBsAg negative healthy blood donors

    OpenAIRE

    Karimi, Gharib; Zadsar, Maryam; Vafaei, Nasrin; SHARIFI, Zohreh; FalahTafti, Mohammad

    2016-01-01

    Background Hepatitis B virus is one of the most important blood born viruses. Although the sensitivity of screening tests has been considerably increased, transmission may still occur due to window period or occult hepatitis B infections (OBIs). This study was aimed at evaluating the prevalence of the anti-HBc and identifying the HBV DNA in HBsAg negative blood donors. Methods The Blood samples from 2031 HBsAg-negative blood donors were divided into three aliquots and tested for anti-HBc, ant...

  10. HCV and HBV coexist in HBsAg-negative patients with HCV viremia; possibility of coinfection in these patients must be considered in HBV-high endemic area

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dong Soon [Korea Cancer Center Hospital, Seoul (Korea, Republic of)

    1998-01-01

    Hepatocellular carcinoma (HCC) is one of the most common cancers and is highly associated with HBV infection in Korea. It has been suggested that HCV core protein may impair the polymerase activity of HBV in vitro, potentially lowering HBV titre in coinfected patients. The aim of this study was to confirm the coexistence of HBV viremia in HCV infected patients HCC who have apparent HBsAg seronegativity. The serological profiles of HBV and HCV in 616 patients with HCC were analysed and coinfection rate of HBV and HCV investigated. Sera were obtained from 16 patients who were both anti-HCV and HCV RNA positive but HbsAg negative, and tested for HBV BY PCR. As a control group, sera were obtained from 15 patients with HCC and 30 non-A abd non-B chronic hepatitis patients without HCC; both were anti-HCV, HCV-RNA, and HBsAg negative and tested for HBV PCR. Of 616 patients with HCC, 450 (73.1 %) had current HBV infection, 48 (7.8 %) had anti-HCV antibodies, and nine (1.5 %) had viral markers of both HCV abd HBV by serological profiles. Of 27 the patients with HCV viremia and HBsAg seronegativity, 14 (51.9 %) showed HBV viremia by PCR. In contrast, of the 75 patients in the control group who were both HCV PCR negative and HBsAg negative, five (11.1 %) showed HBV viremia by PCR. The PCR for HBV revealed coexistent HBV viremia in HCV viremia patients, despite HBsAg negativity by EIA. In HBV-endemic areas, the possibility of coinfection of HBV in HBsAg-negative patients with HCV viremia should be considered and molecular analysis for HBV-DNA performed. (author). 18 refs., 4 tabs.

  11. Prevalence of hepatitis B surface antigen (HBsAg) in blood donors from Bombay.

    Science.gov (United States)

    Satoskar, A; Ray, V

    1992-01-01

    Analysis of serum samples from 3104 blood donors from Bombay screened for hepatitis B surface antigen (HBsAg) by ELISA. HBsAg was detected in 4.7% of the subjects. Relatives showed a significantly higher prevalence of HBsAg than volunteer donors. There was no significant association between HBsAg positivity and a particular blood group.

  12. Clearance of HBV DNA in immunized children born to HBsAg-positive mothers, years after being diagnosed with occult HBV infection.

    Science.gov (United States)

    Sadeghi, A; Yahyapour, Y; Poortahmasebi, V; Shahmoradi, S; Roggendorf, M; Karimzadeh, H; Alavian, S M; Jazayeri, S M

    2016-04-01

    In a previous study, we observed immunoprophylaxis failure due to occult hepatitis B virus (HBV) infection (OBI) despite the presence of adequate levels of anti-HBs in 21 (28%) of 75 children born to HBsAg-positive mothers. The aim of the study was to explore the maintenance of this cryptic condition in this population. Of 21 OBI-positive children, 17 were enrolled. HBV serological profiles were determined by enzyme-linked immunosorbent assay. Highly sensitive real-time and standard PCR followed by direct sequencing were applied in positive cases. The mean age (±SD) of studied patients was 6.57 ± 2.75 years. All children still were negative for HBsAg. All but one (94%) were negative for HBV DNA. Only two children were positive for anti-HBc. The results of the most recent anti-HBs titration showed that 4 (23.5%) and 13 (76.5%) had low (10 IU/mL) levels of anti-HBs, respectively. The only still OBI-positive patient had an HBV DNA level of 50 copy/mL, carried the G145R mutation when tested in 2009 and again in 2013 in the 'a' determinant region of the surface protein. Further follow-up showed that after 18 months, he was negative for HBV DNA. In high-risk children, the initial HBV DNA positivity early in the life (vertical infection) does not necessarily indicate a prolonged persistence of HBV DNA (occult infection). Adequate levels of anti-HBs after vaccine and hepatitis B immune globulin immunoprophylaxis following birth could eventually clear the virus as time goes by. Periodic monitoring of these children at certain time intervals is highly recommended.

  13. Anticuerpo anticitrulina y manifestaciones extra articulares en artritis reumatoidea Anticitrulin antibody and the extra-articular manifestations in rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    María Jezabel Haye Salinas

    2013-02-01

    Full Text Available Los pacientes con artritis reumatidea (AR pueden desarrollar manifestaciones extra articulares (MExA, relacionadas a su morbi-mortalidad. Los anticuerpos anti-péptidos citrulinados cíclicos (ACCP son específicos para la AR y estan relacionados con el daño articular; y podrían tener rol patogénico en las MExA. Nuestro objetivo fue determinar la relación entre los anticuerpos ACCP y MExA en pacientes con AR. Se incluyeron 74 pacientes con diagnóstico de AR (ACR 1987 mayores de 18 años, de más de 6 meses de evolución, con MExA, y un control apareado por sexo y edad sin MExA por cada paciente. Las variables demográficas, clínicas y de laboratorio se compararon con test t, chi cuadrado o Mann-Whitney. Se realizó análisis multivariado; p ≤ 0.05. Los pacientes con MExA presentaron mayor título de anticuerpo ACCP (116 vs. 34, p A large proportion of rheumatoid arthritis (RA patients develop extra-articular manifestations (EAM, which are associated with morbidity and early mortality. Anti cyclic citrullinated peptide (ACCP antibody has proven to be highly specific for the diagnosis of RA, associated with severe joint damage and may have some role in the pathogenesis of EAM. The aim of this study was to determine the relationship between ACCP antibody and the presence of EAM in RA patients. Seventy four RA patients (ACR 1987 with EAM, > 18 years, more than 6 months duration were included, and an EAM free control, matched by sex and age, for each patient. Demographic, clinical and laboratory variables were compared using t-test, chi-square or Mann-Whitney test. Multivariate analysis was performed: p ≤ 0.05. Patients with EAM presented a greater value of ACCP antibody (116 vs. 34, p < 0.01 and rheumatoid factor (108 vs. 34.5, p < 0.01. Independent association with current smoking habit (p = 0.02, OR = 3.78, 95%: 1.17-12.2, RF positive (p = 0.04, OR 3.23, CI 95%: 1.04 to 11.8 and ACCP antibody positive (p = 0.04, OR 3.23, 95% CI: 1

  14. Dose-response characteristics of a monoclonal antibody based two-si immunoradiometric assay for hepatitis B surface antigen (HBsAg)

    International Nuclear Information System (INIS)

    Since the correlation between HBV infectivity and circulating HBsAG was established, increasingly sensitive tests for HBsAg have been developed. Of these, two-site immunoradiometric assays (2S-IRMA) employing polyclonal antibodies of diverse animal origins have proven to be the most sensitive. A number of 2S-IRMA based on mouse monoclonal antibodies have been developed and in 1983 one was released commercially by NML(R). The present study has compared the performance of this assay with that of the polyclonal antibody based HBsAg-assay, AUSRIA(R) II-125, and has looked particularly at dose responsiveness, sensitivity and specificity. The NML(R) monoclonal antibody based assay was found to have the capacity to detect HBsAg at concentrations of 0.31 ng/mL ad and 0.22 ng/mL ay respectively, whereas the polyclonal-based assay (AUSRIA(R) II-125) detected HBsAg as low as 0.09 ng/mL ad and 0.17 ng/mL ay. These findings are contrary to the expectations raised by the claim of significantly improved assay sensitivity with research prototype of the NML(R) assay published by the workers who developed the anti-HBs producing mouse hybridomas

  15. A low proportion of HBeAg among HBsAg-positive pregnant women with known HIV status could suggest low perinatal transmission of HBV in Cameroon

    Directory of Open Access Journals (Sweden)

    Kfutwah Anfumbom KW

    2012-03-01

    Full Text Available Abstract Background Transmission of hepatitis B virus (HBV from HBV-positive mothers to their infants is common and usually occurs when the mother is hepatitis B e antigen (HBeAg positive and/or has a high HBV DNA load. In this study, we determined the prevalence of hepatitis B surface antigen (HBsAg and HBeAg among pregnant women with known HIV status. Findings A total of 650 pregnant women with a mean age of 26.2 years including 301 HIV-positives and 349 HIV-negatives were screened for HBsAg (Monolisa AgHBs Plus Biorad, France. Among the HBsAg-positives, HBeAg and anti-HBe were tested (Monolisa Ag HBe Plus Biorad, France. Overall, 51 (7.85% were positive for HBsAg. The prevalence of HBsAg was not statistically different between HIV-positive and HIV-negative pregnant women [28/301 (9.3% vs 23/349 (6.59%; p = 0.2]. None of the 45 HBsAg-positive samples was reactive for HBeAg. Conclusions Our study indicates a high prevalence of HBsAg with very low proportion of HBeAg in Cameroonian pregnant women. Since perinatal transmission of HBV is mostly effective when the mother is also HBeAg-positive, our data could suggest that perinatal transmissions play a minor role in HBV prevalence in Cameroon. In line with previous African studies, these findings further suggests that horizontal transmission could be the most common mechanism of HBV infections in Cameroon.

  16. Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants

    DEFF Research Database (Denmark)

    Schirmbeck, Reinhold; Dikopoulos, Nektarios; Kwissa, Marcin;

    2003-01-01

    Processing exogenous hepatitis B surface antigen (HBsAg) of the hepatitis B virus (HBV) generates the K(b)-binding S(208-215) epitope 1; processing endogenous HBsAg generates the K(b)-binding S(190-197) epitope 2. Cross-reactive CD8(+) T cell responses were primed to epitope 1 but not epitope 2......-tg mice by HBsAg(adw2); these specific CD8(+) T cells cross-reacted with epitope 1 processed from the transgene-encoded HBsAg(ayw). The liver of vaccinated HBsAg(ayw) transgenic mice showed severe histopathology and contained functional (IFNgamma-producing), cross-reactive CD8(+) T cells, and vaccinated...... HBs-tg mice showed reduced antigenemia. Hence, vaccination with natural HBsAg variants from different HBV sero/genotypes can prime cross-reactive, specific CD8(+) T cell immunity that breaks tolerance to HBsAg....

  17. Protective effect of hepatitis B vaccine combined with two-dose hepatitis B immunoglobulin on infants born to HBsAg-positive mothers.

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    Huaibin Zou

    Full Text Available BACKGROUND: Despite the use of hepatitis B (HB vaccine and hepatitis B immunoglobulin (HBIG, a portion of infants are still non- or low-responders, or even immunoprophylaxis failure. We aimed to determine the immune response in the infants from the mothers being positive for hepatitis B surface antigen (HBsAg, by which the infants received three doses of HB vaccine in combination with two-dose 200 IU HBIG injections. METHODS: In this retrospective study, 621 infants from HBsAg-positive mothers in Beijing YouAn Hospital between January 2008 and December 2009 were included. All the infants were given three doses of 10 µg HB vaccine (at 0, 1 and 6 months of age and two-dose of 200 IU HBIG (at birth and in 2 weeks of age. Serum HBsAg and antibody to HBsAg (anti-HBs in all the infants were determined at 7 months of age. RESULTS: Of the 621 infants, 2.9% were immunoprophylaxis failure (positive for HBsAg, 1.4% were non-responders (anti-HBs undetectable, 95.7% were responders. The 594 responders could be categorized into three subsets, 22 were 10 to 99 IU/L for anti-HBs levels, 191 were 100 to 999 IU/L, and 381 were ≥1000 IU/L. The immunoprophylaxis failure rate was at 0% and 5.2% for the infants of HBeAg-negative and HBeAg-positive mothers(P<0.001. Infants from mothers with detectable HBV DNA had higher incidence of immunoprophylaxis failure than those of mothers without detectable HBV DNA (P = 0.002. The factors including gender, birth weight, gestation weeks, the rates of maternal HBeAg-positive, and detectable HBV DNA did not contribute to the no response to HB vaccination. CONCLUSIONS: Through vaccination by three doses of HB and two-dose of HBIG, majority of the infants (95.7% achieved a protective level of anti-HBs at 7 months of age. Maternal HBeAg-positive and HBV DNA detectable were associated with the immunoprophylaxis failure, but not contribute to the non- or low-response to HB vaccination.

  18. Serological markers of hepatitis B infection in incidentally detected HBsAg positive patients

    International Nuclear Information System (INIS)

    To evaluate the serological profile of the incidentally detected HBsAg positive patients, to asses the severity of disease and to identify the risk factors for the transmission. an observational study. All patients who presented to gastroenterology clinic of Bolan Medical Complex hospital Quetta and at few private clinics with positive HbsAg, detected incidentally, were tested for alamine transaminase (ALT), hepatitis Be antigen (HbeAg) anti. HBe antibody and hepatitis-B virus DNA (HBV DNA) by polymerase chain reaction (PCR). Their risk factors for acquisition of infection were assessed with specific questions. A total of one hundred and four (104) patients with HBsAg reactive were included, 89 were male and 15 were females. The mean age was 30 years, with minimum age of 07 years and maximum of 57 year. Out of 104 patients, 93 patients were for HbeAg, 14 (15%) were positive for HBeAg and 79 (85%) were negative. Anti HBe antibody was checked in 91 patients, and it was reactive in 72 (79%), while it was non-reactive in only 19(21%) patients. HBV DNA (qualitative) determination was done in 95 patients and it was detected in 11(11.6%) ana 84 (88.4%) were negative for HBV DNA. No significant correlation is seen between seroconversion and AL T levels. Normal AL T levels were observed only in 36 patients having seroconversion i.e AntiHBeantibody positive, while it was also normal in 5 HBeAg positive patients. Common risk factors detected in these patients were intramuscular injections, surgery and dental treatment, however, in a large number, risk factors were unknown. Conclusion: Fifteen percent asymptomatic subjects with positive HBsAg were found to be HBeAg positive. Large number of patients 79% has seroconversiqn and only 11.6 % have HBV DNA detected in their serum. Intramuscular injections surgery and dental treatment were noted to be frequent risk factors in these subjects. (author)

  19. Prevalencia de anticuerpos para Trypanosoma cruzi en caninos de dos municipios endémicos de Boyacá

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    Diego Manrique Abril

    2012-04-01

    Full Text Available Objetivo. evaluar la prevalencia de anticuerpos anti Trypanosoma cruzi (T. cruzi en una muestra de caninos domésticos residentes en dos municipios endémicos. Materiales y métodos. Se tomaron muestras séricas de 20 caninos procedentes de hogares donde residen mujeres gestantes seropositivas y 40 perros habitantes de hogares de mujeres gestantes seronegativas en Miraflores y Moniquira, Boyacá. El análisis se realizó mediante prueba diagnóstica rápida dipstick de InBios. Resultados. Se encontró prevalencia del 16.7% en Moniquirá y del 13.3% Miraflores respectivamente con una prevalencia general del 15% en los dos municipios. Se halló riesgo 3 veces mayor de que ocurra la infección en caninos, en los hogares donde residen gestantes seropositivas; además la infestación por pulgas y garrapatas en el animal, hábitat cercano a la vivienda, se relacionan con mayor seropositividad en el canino. Conclusiones. La raza, el sexo, la presencia de aves en la casa y al examen clínico general son considerados factores pronósticos en en la infección por Trypanosoma cruzi en caninos. Como factores protectores se identificó la desparasitación y vacunación de los animales.

  20. Prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre del IMSS, Orizaba, Veracruz, México

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    Ramos-Ligonio Angel

    2006-01-01

    Full Text Available OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70 y un extracto crudo de epimastigotes. Las muestras fueron obtenidas entre los meses de octubre de 2001 a enero de 2002. RESULTADOS: Los 420 donadores de sangre analizados fueron seronegativos para HBV, HCV, BrA, VDRL y HIV. Después del tamizaje de los 420 donadores, se identificaron dos individuos seropositivos por las pruebas de ELISA, Western blot e IFI, con una seroprevalencia de 0.48%. CONCLUSIONES: En este estudio se muestran evidencias de seropositividad para T. cruzi en donadores de sangre del HGRO, lo que sugiere la existencia de riesgo de contaminación por transfusión sanguínea. Por tal motivo, es necesario aplicar programas para el tamizaje serológico a través de técnicas inmunológicas con alta sensibilidad y especificidad.

  1. Detection of HBV DNA by PCR on HBsAg negative blood donors%HBV DNA PCR检测在HBsAg阴性献血人群中的应用

    Institute of Scientific and Technical Information of China (English)

    陈筱华; 林碧; 刘保林; 孔令光

    2008-01-01

    Objective To define the application value of HBV DNA detection on HBsAg-negative blood donors and assess the necessity for nucleic acid detection.Methods Real-time PCR was used to detect HBV DNA on HBsAg negative blood donors.Pools of eight donor samples were used for NAT testing.Viruses were concentrated by centrifugation and the viral DNA extraction was performed using magnetic beads.If HBV DNA wag positive,serological indicators including HBsAg,anti-HBs,HBeAg, anti-Hbe,total anti-HBc was further detected.Results The HBV DNA detection limit was 25 U/ml.There were four HBV DNA positive cases among 23 225 specimens.and the detection rate was 0.17‰ The further serological examination showed anti-Hbe(+),anti-HBc(+) in the two cases and anti-HBc(+) in one case and anti-Hbs(+),anti-HBc(+)in 1 case.The viral load can range form 50 to 200 U/ml. Conclusions The results indicate that there is false negative possibility in blood screening by ELISA.It is necessary to employ anti-Hbe screening or NAT to blood donors screening.%目的 探讨HBsAg阴性献血者HBV DNA榆测的应用价值,评估核酸检测的必要性.方法 采用PCR检测HBsAg阴性献血者HBV DNA.采用8人份混合血样测定,超离心浓缩病毒,磁珠法提取病毒核酸.如HBV DNA为阳性,则进一步检测乙型肝炎病毒血清标志物5项.结果 HBVDNA检测限量为25 U/ml,23 225份标本中有4份为HBV DNA阳性,检出率为0.17‰.进一步检测其他HBV感染的血清学指标,发现这4份标本中有2份为抗HBe和抗HBc阳性,1份为抗HBc阳性,1份为抗HBs、抗HBc阳性.对HBV DNA的定量测定表明,其含量在50~200 U/ml.结论 现行的2次酶联免疫技术的血液筛查存在HBV漏检,有必要在现有的血液筛查模式中增加抗HBc检测,或增加病毒核酸筛查.

  2. Quantitative HBsAg and HBeAg predict hepatitis B seroconversion after initiation of HAART in HIV-HBV coinfected individuals.

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    Gail V Matthews

    Full Text Available OBJECTIVE: Anti-HBe seroconversion and HBsAg loss are important therapeutic endpoints in patients with hepatitis B virus (HBV infection. Quantitative measures of hepatitis B surface antigen (qHBsAg and e antigen (qHBeAg have been identified as potentially useful indicators of therapeutic response in HBV monoinfection. The aim of this study was to examine serological change including quantitative biomarkers in HIV-HBV coinfected patients initiating HBV active antiretroviral therapy (ART. METHODS: HIV-HBV coinfected individuals from Thailand were followed for up to 168 weeks post ART. Rates and associations of qualitative serological change were determined. Longitudinal changes in qHBsAg and qHBeAg were measured and their utility as predictors of response examined. RESULTS: Forty seven patients were included of whom 27 (57% were HBeAg positive at baseline. Median CD4 count was 48 cells/mm(3. Over a median follow-up of 108 weeks 48% (13/27 lost HBeAg, 12/27 (44% achieved anti-HBe seroconversion and 13% (6/47 HBsAg loss. Anti-HBe seroconversion was associated with higher baseline ALT (p = 0.034, lower qHBsAg (p = 0.015, lower qHBeAg (p = 0.031 and greater HBV DNA decline to week 24 (p = 0.045. Sensitivity and specificity for qHBsAg and qHBeAg decline of >0.5 log at week 12 and >1.0 log at week 24 were high for both anti-HBe seroconversion and HBsAg loss. CONCLUSIONS: Rates of serological change in these HIV-HBV coinfected individuals with advanced immunodeficiency initiating HBV-active ART were high. Baseline and on treatment factors were identified that were associated with a greater likelihood of subsequent anti-HBe seroconversion, including both quantitative HBsAg and HBeAg, suggesting these biomarkers may have utility in this clinical setting.

  3. Immuno-potentiating pathway of HBsAg-HBIG immunogenic complex visualized.

    Science.gov (United States)

    Liu, Hu; Geng, Shuang; Wang, Bo; Wu, Bing; Xie, Xiaoping; Wang, Shuang; Zhong, Yiwei; Wang, Xuanyi; Qu, Di; Wen, Yumei; Wang, Bin

    2016-01-01

    Chronic viral hepatitis B (CHB) is a major global health problem. A therapeutic vaccine for CHB comprised of yeast-derived recombinant HBsAg-anti-HBs immunogenic complexes (YIC) has been devloped by us. A series of clinical trials has shown its therapeutic efficacy in decreasing HBV viral load and converting serum HBeAg-positive to anti-HBe-positive status in a subpopulation of CHB patients. Herein, we present a study of the immuno-potentiating mechanisms of YIC revealed by live-cell imaging technology. We studied internalization and dissociation of YIC in cells in vitro, and antigen presentation and T cell stimulation in mice. We found that after YIC was internalized via the Fcγ receptors (FcγR) of antigen presenting cells, it was subsequently transferred through early and late endosomal into lysosomal compartments. The dissociation of YIC was mainly observed in the late endosome. Furthermore when YIC were injected into mice, the populations of IFN-γ- and TNF-α-producing CD8+ and CD4+ T cells were higher in the YIC group than in controls receiving antigen or antibody alone. These observations supplement the known mechanisms of YIC action as a therapeutic vaccine for CHB. PMID:26618396

  4. Helicobacter pylori, hepatitis viruses A, C, E antibodies and HBsAg-prevalence and associated risk factors in pediatric communities of karachi

    International Nuclear Information System (INIS)

    To document the prevalence of Helicobacter pylori (H. pylori), Hepatitis A virus (HAV), Hepatitis C virus (HCV), Hepatitis E virus (HEV) antibodies and Hepatitis B virus surface antigen (HBsAg), in the pediatric age group of low socioeconomic urban communities of Karachi and to identify risk factors associated with these infections. Three hundred and eighty children, ages 5 months to 15 years were investigated. Venous blood samples were collected and questionnaire filled on sociodemographic characteristics (family income, number of dependents in the family, area of living, number of people per room per house, and number of children sharing bed with parents and siblings). Gastrointestinal symptoms were recorded. Anti-HAV IgG (Hepatitis A virus IgG antibody), anti-HCV (Hepatitis C virus antibody), anti-HEV (Hepatitis E antibodies) and HBsAg, were analyzed by enzyme immunoassays (EIAs). Samples were also screened for anti-HIV1/2 (human immunodeficiency virus 1 and 2 antibodies by EIA. IgG antibodies against H. pylori were detected by immunochromatography. A correlation between increasing age and seroconversion was seen for hepatotropic viruses. At 14 years and above,100% of the children were found to be positive for anti-HAV, 26% for anti-HEV, and 1.4%, for anti-HCV while HBsAg was positive in 1.9%. H. pylori infection did not show a significant increase with age. Both anti-HAV and anti-H. pylori were present simultaneously in 30% of the population investigated. With age, increasing number of children acquired antibodies against hepatotropic viruses and H. pylori. Occurrence of HBsAg and anti-HEV at a later age suggests horizontal, rather than vertical transmission. (author)

  5. Immune responses to HBsAg conjugated to protein D of non-typeable Haemophilus influenzae in mice.

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    Qiudong Su

    Full Text Available Hepatitis B vaccine that contains an aluminum hydroxide adjuvant induces apoptotic death of Hepa 1-6 cells. Difficult-to-degrade chemical additives in vaccines effectively enhance vaccine immunogenicity, but also affect the host tissue. Identification of bio-molecules that are readily degraded and compatible in vivo as an adjuvant is important for vaccine research. The hapten-carrier effect suggests that stimulation of helper T (Th cells by carrier adjuvants is feasible. Protein D (PD of non-typeable Haemophilus influenzae covalently conjugated to some polysaccharide vaccines has been confirmed to convert T-cell independent (TI antigens into T-cell dependent (TD antigens, and elicit strong T-cell responses ultimately. Herein, we would substitube PD for aluminum hydroxide adjuvant in Hepatitis B vaccine.Truncated PD (amino acids 20-364 was expressed in Escherichia coli and purified by (NH42SO4 precipitation and DEAE chromatography. After evaluation of antigenicity by western blotting, PD was covalently conjugated to yeast-derived recombinant HBsAg by cross-linking with glutaraldehyde. Intramuscular immunization with the conjugate induced higher level of HBsAg-specific antibody than did HBsAg alone (p < 0.05, and was comparable to commercial Hepatitis B vaccine. During the surveillance period (days 35-105, anti-HBs titers were hold high. Moreover, the conjugated vaccine enhanced Th1 immune responses, while Th2 responses were also activated and induced an antibody response, as determined by IFN-γ ELISPOT and IgG1/IgG2a ratio assays.Recombinant truncated PD covalently conjugated to HBsAg antigen enhanced the immunogenicity of the antigen in mice simultaneously by humoral and cellular immune response, which would facilitate therapeutic hepatitis B vaccines.

  6. Prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre del IMSS, Orizaba, Veracruz, México Prevalence of antibodies against Trypanossoma cruzi in blood bank donors from the IMSS General Hospital in Onizaba, Veracruz, Mexico

    OpenAIRE

    Angel Ramos-Ligonio; Michaía Elián Ramírez-Sánchez; Juan Carlos González-Hernández; José Luis Rosales-Encina; Aracely López-Monteon

    2006-01-01

    OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS) en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO) del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70) y un extracto crudo de epimastigotes. Las muestras fueron o...

  7. A retrospective study of the role of delta agent infection in children with HBsAg-positive chronic hepatitis.

    Science.gov (United States)

    Maggiore, G; Hadchouel, M; Sessa, F; Vinci, M; Craxì, A; Marzani, M D; De Giacomo, C; Alagille, D

    1985-01-01

    The prevalence of intrahepatic delta antigen and/or anti-delta antibody was retrospectively investigated in 102 children with chronic HBsAg-positive hepatitis who were seen consecutively in three medical institutions between 1974 and 1982. Delta infection markers were found in 13 patients (12.7%) who exhibited high serum titers of anti-delta antibody; intrahepatic delta antigen was detected in ten. Eleven of the 13 children had severe progressive liver disease associated in all but one with absence of hepatitis B virus replication as evaluated by analysis of serum hepatitis B virus DNA. The factors which seem to increase the risk of delta infection in children who are hepatitis B virus carriers are geographic origin, a history of exposure to blood derivatives and age. A further 37 of 102 children had chronic active hepatitis (20 patients) or cirrhosis (17 patients) without evidence of delta infection. These results indicate that delta infection occurs in children with chronic hepatitis. This possibility should be considered in investigation of children with HBsAg-positive chronic liver disease. Although the delta agent is an important cause of progressive liver disease in children who are chronic HBsAg carriers, severe liver injury and especially cirrhosis can occur without evidence of delta infection. PMID:3967866

  8. Immune Responses to a Dicistronic Plasmid Expressing HBsAg of Hepatitis B Virus and Interferon-γ

    Institute of Scientific and Technical Information of China (English)

    WANG Chun-yi; QI Feng-chun; WU Xiao-juan; ZHAO Da-peng; LENG Mei; SHENG Jun

    2007-01-01

    DNA vaccines encoding a viral protein have been shown to induce antiviral immune responses and provide protection against subsequent viral challenge. The present article deals with the efficacy of a DNA vaccine greatly improved by the simultaneous expression of HBsAg and interferon-γ gene. We constructed a dual expression vector pHIN encoding the HBsAg of Hepatitis B virus and murine IFN-γ which are connected with Internal Ribosome Entry Site(IRES). Mice inmunized with this dual expression DNA vaccine exhibited the enhancement of cellular immune response and increased the production of anti-HBV surface antibody, compared with the mice of single gene expression control. Taken together, these results demonstrate that the application of a cytokine gene in a DNA vaccine formulation as an adjuvant can improve its immunigenicity.

  9. UltramicroELISA indirecto para la deteccion de anticuerpos totales a citomegalovirus en suero humano Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples

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    Jose Laferte

    1992-02-01

    Full Text Available Se normalizó un ultramicroELISA indirecto para la detección de anticuerpos a Citomegalovirus (CMV humano (UMELISA CMV. Se determinó la concentración óptima de antígeno en 30 ug/ml, la dilución de los sueros fue de 1:40 y la dilución de trabajo del conjugado fue de 1:1500. El UMELISA CMV fue comparado con las técnicas de aglutinación de latex para anticuerpos anti-CMV (Dupont de Neumors y la inmunofluorescencia indirecta (EFT. Los resultados mostraron un alto grado de concordancia y elevada copositividad y conegatividad del UMELISA con respecto a estos dos ensayos. El método es válido para el pesquisaje de anticuerpos en banco de sangre asi como para el diagnóstico de la infección mediante sueros pareados.We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV using human serum samples (UMELISA CMV. The optimal concentration of coating antigen (30 ug/ml, serum dilution (1:40 and anti-human conjugate working dilution (1:1500, were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours and with an indiret immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.

  10. ELISA法检测HBsAg假阳性原因分析%Analysis of ELISA Method for Detection of HBsAg False Positive

    Institute of Scientific and Technical Information of China (English)

    奚焕琴

    2014-01-01

    Objective Detection of HBsAg ELISA method to cause false positive.Methods Col ection of 160 cases of ELISA was detected in HBsAg positive specimens were detected by col oidal gold method, the two do not meet, re were ELISA and col oidal gold method review.Results The initial inspection in 160 HBsAg positive cases and 7 false positive .Conclusion Detection of HBsAg ELISA is af ected by many factors, only to master the detection of HBsAg ELISA factors causing false positive, can reduce errors and accidents at work.%目的了解ELISA法检测HBsAg造成假阳性的原因。方法采集160例ELISA法检测HBsAg阳性标本进行胶体金法检测,二者不符合者,重新抽血进行ELISA和胶体金法复检。结果初检160例HBsAg阳性标本有7例假阳性。结论 ELISA法检测HBsAg受多种因素影响,只有掌握ELISA法检测HBsAg造成假阳性的影响因素,才能在工作中减少差错事故。

  11. Asociación entre enfermedad cardiovascular y anticuerpos contra Chlamydia pneumoniae

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    García-Elorriaga Guadalupe de los A

    2002-01-01

    Full Text Available Objetivo. Precisar si existe asociación entre enfermedad cardiovascular (ECV y anticuerpos contra Chlamydia en población mexicana. Material y métodos. Estudio transversal, realizado en la Unidad de Investigación en Inmunología e Infectología -Hospital de Infectología del Centro Médico Nacional La Raza (CMNR- y en el Servicio de Cirugía Cardiovascular y Asistencia Circulatoria, del Hospital General del CMNR, Instituto Mexicano del Seguro Social (IMSS, de agosto de 1998 a abril de 2000. Se determinaron anticuerpos IgG e IgM contra C. psittaci, C. trachomatis y C. pneumoniae mediante microinmunofluorescencia, en suero de 70 pacientes con ECV hospitalizados en el CMNR, mayores de 30 años, de uno u otro sexo, y se compararon con 140 sanos, pareados por edad y sexo. Se utilizaron muestras aleatorias simples, con un tamaño poblacional de 110, una prevalencia de 50% y un nivel de confianza de 99%. Para establecer la diferencia entre las proporciones de los títulos se utilizó ji cuadrada y se calculó la razón de momios. Resultados. El 94.3% (66/70 de los pacientes presentó IgG en contra de C. pneumoniae vs 37% (52/140 de los individuos sanos (p<0.001. Conclusiones. Existe una fuerte asociación entre anticuerpos IgG hacia C. pneumoniae y ECV.

  12. Suppression of the antigen-specific T cell immune response by co-immunization with the HBV DNA vaccine and recombinant HbsAg%重组质粒与重组蛋白共免疫诱导HBsAg特异性T细胞免疫抑制

    Institute of Scientific and Technical Information of China (English)

    杜小刚; 王军朋; 康有敏; 王肖; 赵干; 王宾

    2009-01-01

    [Objective] To explore a new therapeutic strategy against acute hepatitis B and fulminant hepatitis B, we studied effect of co-immunization with HBV DNA and HBsAg on the T cell proliferation reaction. [Methods] We immunized the BALB/ c mice with HBV DNA vaccine (pcDS2) plus HBsAg by intramuscular injection. The immunization was performed on week 0, 2 and 4. The anti-HBs(IgC)antibody titer, T lymphocyte proliferation reaction , and the expression of IL-10 and Foxp3 in CD3 + T cell were detected on week 6. [Results] The anti-HBs IgC titer induced by pcDS2 plus HBsAg group was higher than that induced by pcDS2, or HBsAg alone. Compared to mice immunized with pcDS2, or HBsAg alone, the stimulated index (SI) of T cell proliferation induced by the pcDS2 plus HBsAg group tested by MTT methods decreased. Besides, the immune suppression of T cell proliferation response induced by co-immunization group was further confirmed by flow cytometry. Finally, the expression of IL-10 and Foxp3 in CD3+ T cell was up-regulated in the co-immunization group significantly. [Conclusion] The co-immunization of HBV DNA vaccine and HBsAg can induce the humoral immune response, but cannot induce antigen specific T cell proliferation reaction. Besides, the immune suppression induced by co-immunization may be correlated with the expression of IL-10 and Foxp3.%[目的]为了探索治疗急性乙型肝炎和爆发性乙型肝的新策略,本研究将HBV DNA疫苗和相应抗原的蛋白质分子联合免疫小鼠,旨在探讨联合免疫对小鼠抗原特异性T细胞增殖反应的影响.[方法]我们将HBV DNA疫苗(pcDS2)和相应抗原蛋白质分子(HBsAg)联合免疫BALB/c小鼠;分别在第0、2和4周进行免疫,在第6周用ELISA方法检测抗-HBs IgG效价,MTr和流式细胞仪检测T细胞增殖反应及流式细胞仪检测细胞因子表达水平.[结果]pcDs2和HBsAg联合免疫组小鼠的抗-HBs水平显著提高;免疫小鼠的T细胞体外经HBsAg刺激后,联

  13. Obtención de anticuerpos policlonales IgY antiparvovirus canino y evaluación en un sistema de aglutinación con látex

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    Jannet González-Figueredo

    2015-12-01

    Full Text Available La parvovirosis canina es una de las principales infecciones que provoca gastroenteritis, fundamentalmente en cachorros, con altos índices de morbiletalidad. Los diagnosticadores más utilizados se basan en la detección de partículas virales excretadas durante la fase aguda de la enfermedad. Algunos requieren equipos especializados, lo que aumenta los costos y el tiempo de diagnóstico. Por esto, la simplificación de estos métodos con alta sensibilidad y especificidad es prioritaria. En Cuba solo se logra un diagnóstico presuntivo sin la completa confirmación de la enfermedad, principalmente por la escasez o ausencia de un medio diagnóstico en toda la red de consultorios y clínicas veterinarias, rápido, eficaz y ajustable a nuestras condiciones. Este trabajo tuvo como objetivo obtener anticuerpos policlonales IgY a partir de yema de huevo de gallina y evaluarlos mediante un sistema de látex-aglutinación, para su posible uso como terapia y principalmente en el diagnóstico. Se inmunizaron por vía intramuscular dos gallinas de raza Leghorn con una cepa atenuada de parvovirus canino (PVC tipo 2. Se aplicaron 8 inoculaciones por ave cada 15 días. Se cosecharon los huevos y se purificaron los anticuerpos por el método de sulfato de dextrana/sulfato de sodio. Se determinó el título de IgY anti-PVC por el método de Inhibición de la Hemoaglutinación (IH y con antígeno de PVC acoplado a partículas de látex de poliestireno. Por IH se obtuvo un título de IgY anti-PVC de 1:1024. Las mezclas de anticuerpos con títulos alto y medio de ambas gallinas aglutinaron con los reactivos preparados con 230 y 460 µg/mL de antígeno. La mayor intensidad de la reacción y la mejor detectabilidad correspondieron a los reactivos elaborados con los anticuerpos de mayor título.

  14. Application of a Newly Developed High-Sensitivity HBsAg Chemiluminescent Enzyme Immunoassay for Hepatitis B Patients with HBsAg Seroclearance

    OpenAIRE

    Shinkai, Noboru; Matsuura, Kentaro; Sugauchi, Fuminaka; Watanabe, Tsunamasa; Murakami, Shuko; Iio, Etsuko; Ogawa, Shintaro; Nojiri, Shunsuke; Joh, Takashi; Tanaka, Yasuhito

    2013-01-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstr...

  15. Establishment and identification of OVA-HBsAg transgenic mice regulated by Cre recombinase

    OpenAIRE

    Xiu-mei LI; Guang-ze LIU; Mei-juan CHEN; Xie, Yong; Kong, Xiang-Ping

    2015-01-01

    Objective To breed OVA-HBsAg transgenic mice regulated by Cre recombinase in order to provide a better animal model for the study of HBV prevention and therapy. Methods The OVA-HBsAg transgenic mice were generated by microinjection of OVA-HBsAg gene with LoxP sites into the pronucleus of C57BL/6J×DBA zygotes. Pups of F1 OVA-HBsAg female mice cross-fertilized with Alb-Cre male mice were assayed for the expression of HBsAg induced by Cre recombinase. PCR, ELISA and immunohistochemical methods w...

  16. Detection of HBs antigen in routine paraffin embedded liver tissue by enzyme-labelled antibody technique

    Directory of Open Access Journals (Sweden)

    Tsuji,Takao

    1976-02-01

    Full Text Available HB surface antigen (HBs Ag was detected using the enzyme-labelled antibody technique on routinely processed liver biopsy material fixed in Bouin's fixative and embedded in paraffin. Of 85 examined specimens, 45 cases were HBs Ag positive by both the immunofluorescent test and the enzyme labelled antibody technique. The remaining 40 cases were negative by both techniques. The specificity of HBs Ag detected by the enzyme-labelled antibody technique was confirmed by the blocking test using guinea pig specific HBs antibody. The results indicate that the enzyme-labelled antibody technique may be useful for detecting HBs Ag on routine paraffin sections.

  17. Anticuerpos monoclonales contra la Gonadotropina Corionica Humana (HCG) y su empleo en el diagnóstico precoz del embarazo.

    OpenAIRE

    Torres E.; N. Pentón; Blanco, R. (Raimundo); N. Abad; A. Triguero; L. Sorell; J. Gavilondo

    2006-01-01

    Se describe la obtención y caracterización de anticuerpos monoclonales contra la hormona gonadotropina cariónica humana (HCG), seleccionados de la inmunización subcutánea en ratones BALB/c. Se reporta también el desarrollo del sistema rápido HeberFast Line® Embarazo, para la detección de la hormona en muestras de orina, basado en los anticuerpos obtenidos y la tecnología del flujo lateral desarrollada en membranas de nitrocelulosa como soporte sólido. El nivel de sensibilidad alcanzado con es...

  18. [Diagnostic value of the quantitative HBsAg determination in hepatitis B infections].

    Science.gov (United States)

    van Helden, J; Weiskirchen, R

    2016-04-01

    Introduction of systematic hepatitis B vaccination has lead to a strong decrease of new infections, but there are still a high numbers of chronically infected persons suffering on long-term complications. Using quantitative assays for the determination of HbsAg (qHBsAg) has improved our understanding of chronic hepatitis B (CHB). The concentrations of HBsAg are strongly varying through the different stages of infection. The quantitative determination of HBsAg does not only yield in additional information to the infection activity, but also provides data for an improved follow up independent from the virus load. As to the prediction of disease progression, low-viremic carriers with high HbsAg levels have been shown to be at higher risk of HBeAg negative hepatitis, cirrhosis and hepatocellular carcinoma (HCC). Although, quantitative HBsAg determination has been widely used in CHB patients receiving pegylated interferon therapy, the HbsAg decline is slow compared to HBV-DNA levels during nucleos(t)ide analogue (NUC) therapy. However a rapid HbsAg decline during NUC therapy may identify patients who will finally clear HbsAg. A 6- to 12-monthly assessment of HbsAg level could be considered during NUC therapy. Taking these lines of evidence together, qHBsAg can complement HBV-DNA levels to optimize the management of CHB patients.

  19. Inmunodeficiencias primarias por déficit de anticuerpos en adultos

    Directory of Open Access Journals (Sweden)

    María Mercedes Bello Rodríguez

    2014-10-01

    Full Text Available Se realizó una investigación sobre los pacientes con inmunodeficiencias primarias por déficit de anticuerpos, atendidos en el servicio de inmunología del Hospital General “Dr. Ernesto Guevara” de Las Tunas, con el objetivo de caracterizar las inmunodeficiencias por déficit de anticuerpos en pacientes adultos, atendiendo al tipo de inmunoglobulina afectada, el sexo y la edad, así como las principales manifestaciones clínicas. Se evaluaron un total de diez pacientes tratados por esta causa entre los años 2005 y 2013. Para la evaluación de los resultados se utilizó el análisis de frecuencias simple. Las inmunodeficiencias diagnosticadas se correspondieron en un 50% con el déficit de las tres clases de inmunoglobulinas. Predominó el sexo masculino en un 70% y las edades más afectadas se correspondieron con la cuarta y quinta décadas de la vida. Las infecciones respiratorias y digestivas fueron las más frecuentes (80%, tanto por gérmenes bacterianos como virales, la infección por Giardia lamblia también apareció muy frecuentemente en los pacientes estudiados

  20. Frecuencia de anticuerpos contra el virus C de la hepatitis en pacientes con cirrosis hepática en Yucatán, México

    Directory of Open Access Journals (Sweden)

    Góngora-Biachi Renán A

    2003-01-01

    Full Text Available OBJETIVO: En este estudio reportamos la prevalencia de anticuerpos contra el virus C de la hepatitis (Ac-VCH en un grupo de pacientes con cirrosis hepática (CH. MATERIAL Y MÉTODOS: Se hizo un estudio prospectivo, transversal y descriptivo, de marzo de 1998 a mayo de 1999. Se estudiaron a 153 pacientes (117 (76% hombres y 36 (24% mujeres con diagnóstico de CH, que eran atendidos en el Hospital General Agustín O' Horan y en el Centro de Investigaciones Regionales Doctor Hideyo Noguchi, en la ciudad de Mérida, Yucatán, México. Se aplicó un cuestionario con datos clínico-epidemiológicos y se determinó la presencia de Ac-VCH (ELISA de 2ª generación y RIBA-2 para confirmar el diagnóstico a cada paciente. Se determinó también el antígeno de superficie de la hepatitis B (AgsHB y anticuerpos contra el antígeno central de la hepatitis B (Anti-HBc mediante el método de ELISA. La presencia de Ac-VCH fue relacionada con las variables epidemiológicas de los sujetos. La prevalencia de anti-HCV y la frecuencia de características se compararon entre los pacientes positivos y negativos con las pruebas de c² y exacta de Fisher. RESULTADOS: El 32% de los pacientes con CH (35/117 (30% hombres y 14/36 (39% mujeres fueron positivos para los Ac-VCH. El alcoholismo estuvo presente en todos los hombres serorreactivos y en ninguna de las mujeres positivas (p< 0.001. Ninguna de las variables epidemiológicas analizadas estuvo asociada con la seropositividad. Los anti-HBc se encontraron en 16% de los pacientes positivos para Ac-VCH y en 12% de los seronegativos (p=0.69. CONCLUSIONES: La prevalencia encontrada fue mayor a los reportes previos realizados en población general de la Península de Yucatán (1.3%. La alta prevalencia de Ac-VCH en este grupo de pacientes sugiere que la CH está más frecuentemente asociada con el VCH en Yucatán, México, que con el virus B de la hepatitis. El alcoholismo probablemente actúa como un cofactor en el

  1. A comparison on HBsAg and HBsAb prevalence in patients with Down syndrome and other mentally retarded patients living in retarded children institutes in Tehran

    Directory of Open Access Journals (Sweden)

    Sahebjamei M. Associate Professor

    2003-06-01

    Full Text Available Statement of Problem: The Prevalence of HBsAg, among patients with Down Syndrome (DS has been"nreported higher than normal population. Living in institutes, comparing to home, leads to higher"nprobability of HBsAg prevalence."nAim: The purpose of the present study was to determine HBsAg prevalence in patients with DS and"ncompare it with other mentally retarded patients with similar conditions, and also with healthy population"nin Iran."nMaterials and Methods: One hundred patients, with average age of 12 years, living in five institutes in"nTehran, were chosen. Children with DS and other mentally retarded children were considered as the first"nand second groups, respectively (50 members in each group. Blood samples were taken and tested"n(ELLSA to determine HBsAg and HBsAb titre. For statistical analysis, Chi- Square test was used."nResults: HBsAg was positive in 8% and 4% of DS and MR groups, respectively. HBsAb was seen in"n25% of DS, comparing to 32% of MR. The prevalence of HBsAg in retarded patients living in institutes"nwas higher than normal population of Iran (less than 3%, which was not statistically significant."nConclusion: Consideration of sterilization principles and infection control upon dental operations, in"nthese centers, are necessary. It is also suggested that these children, as a high- risk population, should be"nvaccinated against hepatitis B.

  2. Serologic tracers (HBsAg and HBsAc) of hepatitis B virus in expectant mothers of the Perinatal Maternal Institute

    CERN Document Server

    Garcia, B M

    1997-01-01

    A study on hepatitis B tracers, (HBsAg and HBsAc), was conducted in women with different months of pregnancy at the Perinatal Maternal Institute in Lima, Peru. A total of 1010 mothers were studied during the period of January to October 1996, establishing by radioimmunoassay (RIA) whether they were positive or not. The results showed a prevalence rate of 1,6 for HBsAc and 1,3 for HBsAg for every 100,000 inhabitants. The incidence rate was 0,332 for HBsAc and 0,07 for HBsAg for every 100,000 inhabitants. This means that 21 expectant mothers are HBsAc positive and 5 are HBsAg positive. According to the investigations, there were different ways of transmission; promiscuity must be highlighted, as well as age -most of the mothers who were positive were between 20 and 25 years old- and origin. Most of them were immigrants from different places and live in shantytowns, which indicates that most of them have limited economic resources and have not received any orientation on family planning.

  3. Serologic tracers (HBsAg and HBsAc) of hepatitis B virus in expectant mothers of the Perinatal Maternal Institute

    International Nuclear Information System (INIS)

    A study on hepatitis B tracers, (HBsAg and HBsAc), was conducted in women with different months of pregnancy at the Perinatal Maternal Institute in Lima, Peru. A total of 1010 mothers were studied during the period of January to October 1996, establishing by radioimmunoassay (RIA) whether they were positive or not. The results showed a prevalence rate of 1,6 for HBsAc and 1,3 for HBsAg for every 100,000 inhabitants. The incidence rate was 0,332 for HBsAc and 0,07 for HBsAg for every 100,000 inhabitants. This means that 21 expectant mothers are HBsAc positive and 5 are HBsAg positive. According to the investigations, there were different ways of transmission; promiscuity must be highlighted, as well as age -most of the mothers who were positive were between 20 and 25 years old- and origin. Most of them were immigrants from different places and live in shantytowns, which indicates that most of them have limited economic resources and have not received any orientation on family planning

  4. Comparison of serum HBsAg quantitation by four immunoassays, and relationships of HBsAg level with HBV replication and HBV genotypes.

    Directory of Open Access Journals (Sweden)

    Edouard Tuaillon

    Full Text Available BACKGROUND: The decline in hepatitis B virus surface antigen (HBsAg may be an early predictor of the viral efficacy of Hepatitis B virus (HBV therapy. The HBsAg levels obtained by different immunoassays now need comparing and the relationships between levels of HBsAg and HBV DNA alongside HBsAg and genotype must be evaluated. METHODOLOGY/PRINCIPAL FINDINGS: HBsAg levels were compared among 80 patients using the Abbott Architect assay, a commercial immunoassay approved for HBsAg detection and quantitation, and three other assays derived from immunoassays approved for HBsAg detection (manufactured by Diasorin, Bio-Rad and Roche. Good correlation was found between the Abbot vs. Diasorin, Bio-Rad and Roche assays with narrow 95% limits of agreement and small mean differences: -0.06 to 0.11, -0.09 log(10 IU/mL; -0.57 to 0.64, -0.04 log(10 IU/mL; -0.09 to 0.45, -0.27 log(10 IU/mL, respectively. These agreements were not affected by genotypes A or D. HBsAg was weakly correlated with HBV DNA, whatever the HBsAg assay used: Abbott, ρ = 0.36 p = 0.001, Diasorin ρ = 0.34, p = 0.002; Bio-Rad ρ = 0.37, p<0.001; or Roche ρ = 0.41, p<0.001. This relationship between levels of HBsAg and HBV DNA seemed to depend on genotypes. Whereas HBsAg (Abbott assay tended to correlate with HBV DNA for genotype A (ρ = 0.44, p = 0.02, no such correlation was significant for genotypes D (ρ = 0.29, p = 0.15. CONCLUSION/SIGNIFICANCE: The quantitation of HBsAg in routine clinical samples is comparable between the reference assay and the adapted assays with acceptable accuracy limits, low levels of variability and minimum discrepancy. While HBsAg quantitation is not affected by HBV genotype, the observed association between levels of HBsAg and HBV DNA seems genotype dependent.

  5. OBTENCIÓN DE ANTICUERPOS POLICLONALES IgY ANTIPARVOVIRUS CANINO A PARTIR DE YEMA DE HUEVO DE GALLINA

    Directory of Open Access Journals (Sweden)

    Pinto, J.

    2005-06-01

    Full Text Available Inmunizando un grupo de diez gallinas de postura raza Lohmann de 16 semanas de edad con parvovirus canino (CPV cepa vacunal, se obtuvieron anticuerpos IgY policlonales contra CPV en yema de huevo.En la extracción de los anticuerpos de la yema se requirieron dos pasos, el primero para la remoción de lípidos y el segundo para la precipitación de proteínas. Para la remoción de lípidos se usó el método PBS:Cloroformo y para la precipitación de los anticuerpos solubles (Inmunoglobulinas-IgY se usó el método de salting-out con sulfato de amonio ((NH42SO4. La evaluación del proceso se efectuó empleando el método comercial estándar “EGGstract IgY Purification System®” de PROMEGA.La metodología empleada permitió la obtención de anticuerpos IgY policlonales contra CPV a partir de yema de huevo de gallina en concentraciones altas por mililitro de yema, con una pureza aceptable y títulos altos; los resultados fueron comparables con el método comercial.

  6. Establishment and identification of OVA-HBsAg transgenic mice regulated by Cre recombinase

    Directory of Open Access Journals (Sweden)

    Xiu-mei LI

    2015-06-01

    Full Text Available Objective To breed OVA-HBsAg transgenic mice regulated by Cre recombinase in order to provide a better animal model for the study of HBV prevention and therapy. Methods The OVA-HBsAg transgenic mice were generated by microinjection of OVA-HBsAg gene with LoxP sites into the pronucleus of C57BL/6J×DBA zygotes. Pups of F1 OVA-HBsAg female mice cross-fertilized with Alb-Cre male mice were assayed for the expression of HBsAg induced by Cre recombinase. PCR, ELISA and immunohistochemical methods were used to detect the integration and expression of HBsAg gene and Cre gene in the transgenic mice. Results 491 fertilized eggs were injected and a total of 337 survived. The survival rate of injection was 68.6%. 29 F0 pups were produced with 4 PCR-positive mice, and the positive rate was 13.8%. Up to now, F4 pups were obtained. The positive rates from F1 to F4 were 27.5%, 32.0%, 22.9% and 25.0%, respectively. No HBsAg-positive mice were found among these pups. Furthermore, among 16 pups of F1 OVA-HBsAg female mice crossed with Alb-Cre male mice, 6 were positive for both HBsAg and Cre as detected by PCR. There were 2 pups showed positive HBsAg by ELISA assay. The expression rate of HBsAg after Cre recombinase induction was 33.3%. Conclusion OVA-HBsAg transgenic mice has been reproduced successfully, and it can be stably passaged. Cre recombinase can induce the expression of HBsAg in vivo. DOI: 10.11855/j.issn.0577-7402.2015.05.08

  7. Perfil infectológico de poblaciones mapuche de Cerro Policía y Aguada Guzmán de la provincia de Río Negro

    OpenAIRE

    Rey, Jorge Alberto; Dejean, Cristina Beatriz; Niborski, Ricardo; Arrayago, Alicia M.; Kohan, Abraham I.; Carnese, Francisco R.

    1996-01-01

    Nuestro trabajo analiza la prevalencia de marcadores de algunas infecciones que afectan las poblaciones mapuche de Cerro Policía (CP) y Aguada Guzmán (AG) en la Prov. de Río Negro. Los estudios incluídos son: determinación de anticuerpos anti-Treponema pallidum, anti-Trypanosoma cruzii, anti-Echinococcus granulosus, antígeno de superficie de la Hepatitis B (HBsAg), anticuerpos contra el core de la Hepatitis B (anti-HBc), anticuerpos contra el virus de la Hepatitis A (anti-HAV). Se estudiaron ...

  8. Anticuerpos séricos contra la enfermedad de Newcastle e Influenza Aviar en aves rapaces de Chile.

    Directory of Open Access Journals (Sweden)

    Daniel González-Acuña

    2012-12-01

    Full Text Available Objetivo. Detectar la presencia de anticuerpos séricos sanguíneos contra los virus de la Enfermedad de Newcastle (ENC e Influenza aviar (IA, para comprender la contribución de las aves silvestres en la transmisión de estos virus en Chile. Materiales y métodos. Se analizaron 63 aves pertenecientes a los órdenes Falconiformes y Strigiformes desde centros de rehabilitación de aves de las zonas central y sur de Chile. Se realizaron las pruebas de inhibición de la hemoaglutinación (IHA para detectar anticuerpos contra el virus ENC e inmunodifusión en gel agar (IDGA y ELISA para IA. Resultados. Se detectaron 14 aves positivas (22.2% para anticuerpos séricos contra el virus de la ENC. En cambio, no se registraron anticuerpos séricos sanguíneos para el virus de la IA. Conclusiones. La presencia de aves rapaces positivas en los centros de rescate a los anticuerpos séricos contra el virus de la ENC puede ser explicada por el consumo de carne de pollos que han sido vacunados contra ENC o consumo de aves que han adquirido directamente el virus vacunal a través de los distintos procedimientos de administración (aerosoles, bebederos de la vacuna o por el ingreso a los centros de rescate de aves rapaces migratorias, las que podrían facilitar la diseminación de la infección desde los países de origen, hecho que debe ser investigado.

  9. Survey of both hepatitis B virus (HBsAg and hepatitis C virus (HCV-Ab coinfection among HIV positive patients

    Directory of Open Access Journals (Sweden)

    Pournia Yadollah

    2009-11-01

    Full Text Available Abstract Background HIV, HBVand HCV is major public health concerns. Because of shared routes of transmission, HIV-HCV coinfection and HIV-HBV coinfection are common. HIV-positive individuals are at risk of coinfection with HBV and HCV infections. The prevalence rates of coinfection with HBV and HCV in HIV-patients have been variable worldwide depending on the geographic regions, and the type of exposure. Aim This study aimed to examine HBV and HCV coinfection serologically and determine the shared and significant factors in the coinfection of HIV-positive patients. Methods This descriptive, cross-sectional study was carried out on 391 HIV-positive patients including 358 males and 33 females in Lorestan province, west Iran, to survey coinfection with HBsAg and anti-HCV. The retrospective demographic data of the subjects was collected and the patients' serums were analyzed by ELISA kits including HBsAg and anti-HCV. The collected data was analyzed with SPSS software (15 and Chi-square. Fisher's exact test with 5% error intervals was used to measure the correlation of variables and infection rates. Results The results of the study indicated that the prevalence of coinfection in HIV-positive patients with hepatitis viruses was 94.4% (370 in 391, out of whom 57 (14.5% cases were HBsAg positive, 282 (72% cases were anti-HCV positive, and 31 (7.9% cases were both HBsAg and anti-HCV positive. Conclusion There was a significant correlation between coinfection with HCV and HBV and/or both among HIV-positive patients depending on different variables including sex, age, occupation, marital status, exposure to risk factors.(p

  10. Producción de un anticuerpo IgY específico contra el antígeno CD41 humano

    OpenAIRE

    Esteban J. Gutiérrez Calzado; Marlene Toledano Heredia; Hans Bäumler; Rüdiger Schade

    2009-01-01

    Desde hace dos décadas, la comunidad científica muestra un gran interés hacia la generación de anticuerpos en gallinas ponedoras por las ventajas que estas aportan al compararse con otros métodos tradicionales para este fin, sobre todo, en el hecho de lograrse en ellas, mejores respuestas de anticuerpos hacia antígenos de mamíferos muy conservados en la filogenia entre los que se encuentran glicoproteínas de membrana celular. El objetivo de este trabajo fue la generación de anticuerpos especí...

  11. 阻断PD-1/PD-L1信号通路对负载HBsAg的树突细胞抗HBV免疫功能的影响%Effect of programmed death-1/progranuned death ligand-1 interaction on immune response of HBsAg-pulsed denddtic cells to hepatitis B virus

    Institute of Scientific and Technical Information of China (English)

    郭忠胜; 潘修成; 陈明; 杨帆; 李丽; 张言超; 李秀华

    2010-01-01

    Ag protein.HBV transgenic mice were injected i.D with PD-L1 blocking monoclonal antibodies(Abs)1 d before DC/HBsAg transfer with an interval of 3 d for 3 times,and then divided into 5 groups:DC/HBsAg+PD-L1 Abs group,DC/HBsAg group,DC group,mlgG group and phosphate buffered saline(PBS)group.Extracellular molecule and intraceilular cytokine of splenic T cells were analyzed by flow cytometry,HBsAg-specific activity of splenic cytotoxic T lymphocytes (CTLs)was detected by LDH release assay,HBsAg and HBV DNA titers in sera were monitored by ELISA and fluorescent quantitative PCR,and hepatic injury Was monitored by measuring serum ALT at 3th and 6th after immunization,respectively.SPSS 13.0 software Was used for data processing,and ANOVA(analysis of variance)as well as q test were performed to compare the measurement data among the groups.Results At 3th and 6th after immunization,PD-L1 blockade stimulated splenic CD_3~+CD_8~+T lymphocytes proliferation more efficiently than that with no PD-L1 blockade(mice with DC/HBsAg treatment alone)(F=25.22 and 39.01,P<0.01),and the secretion of IFNγ WaS hisher(F=32.35 and 36.98.P<0.01);meanwhile the differences between mice with DC/HBsAg treatment and those with DC alone were only observed at 3th(t=6.79,P=0.012).The activity of HBsAg-specific CTL and serum HBsAg levels at 6th were significantly higher in mice with anti-PD-L1 Abs treatment compared with those in other groups (F=6.12,P<0.05).Serum ALT levels in mice with anti-PD-L1 Abs treatment were the same with DC/HBsAg group at 3th,but were higher than those in other groups at both 3th and 6th(F=19.22 and 14.30,P<0.05).All groups were similar in the clearance of HBV DNA.but HBV DNA hvel decreased one log copy in one mouse with anti-PD-L1 Abs treatment.Conclusion Blocking PD-1/PD-L1 interaction can enhance specific CD_3~+CD_8~+ T lymphocyte proliferation and the capability of secreting IFNγ,which improves the inhibition of HBV in transgenic mice induced by HBsAg-pulsed DC vaccine.

  12. 麻鸭血替代人血测定HBsAg-ELISA法在校内实训中的应用研究%The Research of The Testing Way of HbsAg - ELISA with Ma Duck Blood Replacing Human Blood in the training Courses in Our College

    Institute of Scientific and Technical Information of China (English)

    吴展奎; 宋爱萍

    2012-01-01

    为了避免实验室生物安全事故(学生感染和实验室污染)的发生,探讨采用麻鸭血替代人血用酶联免疫吸附试验(ELISA法)作乙肝病毒表面抗原(HBsAg)检测的可行性。方法:收集黔东南地区100份麻鸭血标本,采用ELISA法检测HBsAg。结果 100份麻鸭血清标本HBsAg检测结果均为阴性。结论:我国多地报道麻鸭血携带鸭乙肝病毒(DHBV),但通过检测,我地区麻鸭血HBsAg为阴性,在校内实训课中可作为标本应用。%purpose In order to avoid the accidents (infection on the students and pollution to the lab) in the biological lab, we have explored the possibility of testing Hepatitis B virsu Surface Anti C, en(HbsAg) in the way of Enzyme Linked Immunosorbent Assay ( ELISA), with the Ma Duck blood replacing the human blood. Methodology Collect the blood from 100 Ma ducks in the area of Qiandongnan as the samples, then test HBsAg using the way of ELISA. Results All the blood samples from the 100 Ma ducks show the test resuls are negative. Conclusion As reported, Ma duck blood carries the Duck Hepatitis B Virus (DHBV) in most areas of China, however, Ya duck blood in the area of Qiandongnan is negative in the test, so it can be appiled for samples in the training courses.

  13. Construction and characterization of calreticulin-HBsAg fusion gene recombinant adenovirus expression vector

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine.METHODS: CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease diges-tion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5′ end. Adenoviral expression vector containing CRT-HBsAg fusion gen...

  14. HBS: a novel biometric feature based on heartbeat morphology.

    Science.gov (United States)

    Islam, Md Saiful; Alajlan, Naif; Bazi, Yakoub; Hichri, Haikel S

    2012-05-01

    In this paper, a new feature named heartbeat shape (HBS) is proposed for ECG-based biometrics. HBS is computed from the morphology of segmented heartbeats. Computation of the feature involves three basic steps: 1) resampling and normalization of a heartbeat; 2) reduction of matching error; and 3) shift invariant transformation. In order to construct both gallery and probe templates, a few consecutive heartbeats which could be captured in a reasonably short period of time are required. Thus, the identification and verification methods become efficient. We have tested the proposed feature independently on two publicly available databases with 76 and 26 subjects, respectively, for identification and verification. The second database contains several subjects having clinically proven cardiac irregularities (atrial premature contraction arrhythmia). Experiments on these two databases yielded high identification accuracy (98% and 99.85%, respectively) and low verification equal error rate (1.88% and 0.38%, respectively). These results were obtained by using templates constructed from five consecutive heartbeats only. This feature compresses the original ECG signal significantly to be useful for efficient communication and access of information in telecardiology scenarios. PMID:22361664

  15. Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells

    Institute of Scientific and Technical Information of China (English)

    Shan Qin; Hong Tang; Lian-San Zhao; Fang He; Yong Lin; Li Liu; Xiao-Mei He

    2003-01-01

    AIM: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monoclone SP2/0 cell strains that are capable of expressing S or S2S proteins stably.METHODS: Segments of S, preS2-S, preS1-preS2-S genes of Hepatitis B virus were amplified by routine PCR and preS1S fragment was amplified by Over-Lap Extension PCR. The amplified segments were cleaved with restricted endonuclease Hind Ⅲ/Not Ⅰ followed by ligation with pRc/CMV, or BamHI/EcoR Ⅰ followed by ligation with pSG5UTPL/Flag. After the plasmid vectors were cleaved with the correspond enzymes, the amplified segments were inserted into pRc/CMV or pSGSUTPL/Flag plasmid vectors with T4DNA ligase. KOZAK sequence was added before the initial ATG code of each fragment using specific primer. The inserted segments in the recombinant plasmids were sequenced after subcloning. BALB/c mice myeloma cells (SP2/0 cell line) were transfected with the recombinant plasmids. The expressions of the different recombinants were compared by Western-blot, using a monoclonal anti-HBs antibody as the primary antibody and peroxidase-labeled multi-linker as the secondary. Stable SP2/0-pRc/CMV-S or SP2/0- pRc/CMV-MS clones were established through clone screening with G418.RESULTS: Fragments with anticipated size were harvested after PCR. After recombination and screening, the sequences of the inserted segments in the recombinants were confirmed to be S, preS2S, preSl-preS2S and preSlS encoding genes,determined by sequencing. The results of Western-blot hybridization were positive for the anticipated proteins.Among them, pRc/CMV-S or pRc/CMV-MS demonstrated the highest expressing their respective antigen.CONCLUSION: Eight recombinant plasmids expressing S,M, L or preSlS proteins are obtained. For hepatitis surface antigen expression in eukaryotic cells

  16. Enhanced HBsAg synthesis correlates with increased severity of fibrosis in chronic hepatitis B patients.

    Directory of Open Access Journals (Sweden)

    Mei-Zhu Hong

    Full Text Available BACKGROUND AND AIMS: Little is known about whether low serum HBsAg levels result from impaired HBsAg synthesis or a reduced number of hepatocytes caused by advanced liver fibrosis. Therefore, we investigated the capacity for HBsAg synthesis in a cross-sectional cohort of treatment-naïve chronic hepatitis B patients. METHODS: Chronic hepatitis B patients (n = 362 were enrolled; liver biopsies were performed and liver histology was scored, and serum HBsAg and HBV DNA levels were investigated. In the enrolled patients, 183 out of 362 have quantitative serum HBsAg levels. Tissue HBsAg was determined by immunohistochemistry. RESULTS: A positive correlation between serum HBsAg and HBV DNA levels was revealed in HBeAg(+ patients (r = 0.2613, p = 0.0050. In HBeAg(+ patients, serum HBsAg and severity of fibrosis were inversely correlated (p = 0.0094, whereas tissue HBsAg levels correlated positively with the stage of fibrosis (p = 0.0280. After applying the mean aminopyrine breath test as a correction factor, adjusted serum HBsAg showed a strong positive correlation with fibrosis severity in HBeAg(+ patients (r = 0.5655, p<0.0001. The adjusted serum HBsAg values predicted 'moderate to severe' fibrosis with nearly perfect performance in both HBeAg(+ patients (area under the curve: 0.994, 95% CI: 0.983-1.000 and HBeAg(- patients (area under the curve: 1.000, 95% CI: 1.000-1.000. CONCLUSIONS: Although serum HBsAg levels were negatively correlated with fibrosis severity in HBeAg(+ patients, aminopyrine breath test-adjusted serum HBsAg and tissue HBsAg, two indices that are unaffected by the number of residual hepatocytes, were positively correlated with fibrosis severity. Furthermore, adjusted serum HBsAg has an accurate prediction capability.

  17. Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg

    Institute of Scientific and Technical Information of China (English)

    Yi Gao; Yina Ma; Mei Li; Tonq Chenq; Shao-Wei Li; Jun Zhang; Ning-Shao Xia

    2003-01-01

    AIM: To investigate the expression of recombinant HBsAg (rHBsAg) in transgenic cherry tomatillo in order to explore the feasibility of producing HBV oral vaccine with cherry tomatillo by animal immune tests.METHODS: The recombinant plant expression vector containing HBsAg gene was constructed. Mediated with Agrobacterium tumefaciens, HBsAg gene was transferred into cotyledons of cherry tomatillo. Transformed cherry tomatillos were obtained through hygromycin delay-selection. Integrated DNA in transgenic cherry tomatillo was confirmed by hygromycin resistance selection, Gus detection, polymerase chain reaction (PCR) and dot blotting analysis. Antigenicity of rHBsAg was examined by ELISA and the immunogenicity of rHBsAg derived from transgenic cherry tomatillo tissues was confirmed by oral feed of transformed tissues to BALB/c mice primed with commercial HBV vaccines. Specific antibody titers in mice's serum were examined by ELISA every week.RESULTS: By far, 10 positive lines of transgenic cherry tomatillos containing HBsAg gene were obtained. Among different organs of the same transgenic cherry tomatillo,level of rHBsAg expressed in leaves was the highest with the yield up to 300ng/g fresh weight. And the rHBsAg expression level in fruits was about 10 ng/g fresh weight.In animal immune tests, oral delivery with transgenic tissues to mice primed with commercial vaccine instead of naive mice resulted in significant immune response.CONCLUSION: The result of this animal immune test indicated the rHBsAg derived from transgenic cherry tomatillo possessed normal immunogenicity. This work demonstrated the feasibility to generate oral immunogenic rHBsAg in transgenic cherry tomatillo, and would provide some experimental approach for the production of low-cost oral vaccines using transgenic cherry tomatillo in large scale.

  18. Study on the risk factors of HBV infection among spouses of HBsAg carriers%乙型肝炎病毒表面抗原携带者配偶感染乙型肝炎危险因素调查

    Institute of Scientific and Technical Information of China (English)

    王青; 徐佳薇; 姚宁

    2014-01-01

    目的:了解乙型肝炎病毒(HBV )表面抗原(HBsAg )携带者配偶 HBV感染状况及影响因素,探讨其有效的防控策略,降低HBV性接触传播的风险。方法采用1∶2病例对照研究设计,运用ELISA对2005年全国乙型肝炎血清学调查中重庆市18~59岁人群HBsAg携带者的配偶、健康人群的配偶开展HBV血清学(HBsAg、抗-HBc、抗-HBs)检测。结果病例组(HB-sAg携带者)HBsAg阳性率(14.8%)明显高于对照组(健康人群的配偶,7.5%),P<0.01;女性配偶其HBsAg阳性率随结婚年限增加呈上升趋势;多因素分析发现,配偶为HBsAg携带者、不使用安全套是HBsAg感染的危险因素。结论 HBsAg携带者配偶感染HBV风险高;性接触传播中,女性较男性更容易感染乙型肝炎,提倡婚前体检,采取安全性行为或配偶及时接种乙型肝炎疫苗等有效措施以降低 HBV性接触传播。%Objective To investigate the risks of HBV infection among the spouses of HBV surface antigen (HBsAg)carriers and to find out effective control strategies on hepatitis B control and prevention .Methods To use case-control study(1∶2) including spouses of HBsAg carriers aged 18 -59 years-old from the nationwide sero-epidemiological survey for Hepatitis B in Chonqing province in 2005 ,and the spouses of the healthy(HBsAg negative persons)as the control groups ,adopt euzymelinked immunosor-bent assay(ELISA) to carry out the sero-epidemiological testing (HBsAg ,anti-HBc ,anti-HBs) for Hepatitis B .Results The posi-tive rate of HBsAgamong the spouses of HBsAg carriers (14 .8% ) was higher than the rate of spouse among the healthy (7 .5% ) , with difference statistically significant (P<0 .01) ,the positive rate of HBsAg in female spouse was uptrend as the marriage age grown ,multiple factor analysis found that the risks of HBV infection among the spouses were their spouse with HBsAg and without condom when the sexual

  19. [Comparison of the clinical performance of the ECLusys HBsAg II assay with the Lumipulse f and HISCL 2000-i HBsAg screening assays].

    Science.gov (United States)

    Sugiura, Aya; Iwahara, Kunihiro; Suga, Yasuyuki; Uchiyama, Sachinori; Maekawa, Masato

    2012-02-01

    We compared the ECLusys HBsAgII (ECL HBsAg) assay to the Lumipulse Forte (LPf HBsAg) and HISCL (HIS HBsAg) assays. Measurement of dilution panels for which the WHO HBsAg international reference panel was the parent specimen revealed that the ECL and HIS assays enabled detection to a theoretical level of 0.04 IU/mL, whereas the LPf assay enabled detection to a level of 0.08 IU/mL. In a specificity test using high RF positive specimens (n = 33), pregnancy specimens (n = 35), cytomegalovirus antibody positive specimens (n = 36), and high M protein positive specimens (n = 21) that were confirmed negative for HBsAg by the LPf assay, negative results were obtained for all specimens on the HIS assay, but the ECL assay yielded a positive result for one of the high RF positive specimens. This individual was suggested on further testing to be an HBV carrier who was strongly positive for HBc antibody. In HBsAg mutants detection test, the detection rate was 92.3% with the ECL assay and 69.2% with the HIS assay. In a correlation test using routinely collected clinical specimens (n = 155), including positive stock specimens, aside from the one case where the LPf assay gave a negative result but both the ECL and HIS assays gave positive results, all of the results were consistent for all specimens. The above results confirmed that the ECL assay is both highly sensitive and specific, and also enables a high rate of HBsAg mutant detection.

  20. Anti-HBc screening in Indian blood donors: Still an unresolved issue

    Institute of Scientific and Technical Information of China (English)

    Hari Krishan Dhawan; Neelam Marwaha; Ratti Ram Sharma; Yogesh Chawla; Beenu Thakral; Karan Saluja; Sanjeev Kumar Sharma; Manish K Thakur; Ashish Jain

    2008-01-01

    AIM:To study the seroprevalence of antibody to hepatitis B core antigen (anti-HBc) in healthy blood donors negative for HBsAg and to evaluate whether anti-HBc detection could be adopted in India as a screening assay for HBV in addition to HBsAg.METHODS:A total of 1700 serum samples collected from HBsAg-negative healthy blood donors were tested for the presence of anti-HBc antibody (IgM + IgG).All samples reactive for anti-HBc antibody were then investigated for presence of anti-HBs and for liver function tests (LFTs).One hundred serum samples reactive for anti-HBc were tested for HBV DNA by PCR method.RESULTS:Out of 1700 samples tested,142 (8.4%)blood samples were found to be reactive for anti-HBc.It was significantly lower in voluntary (6.9%) as compared to replacement donors (10.4%,P=0.011).Seventytwo (50.7%) anti-HBc reactive samples were also reactive for anti-HBs with levels >10 mIU/mL and 70(49.3%) samples were non-reactive for anti-HBs,these units were labeled as anti-HBc-only.These 142 anti-HBc reactive units were also tested for liver function test.HBV DNA was detected in only 1 of 100 samples tested.CONCLUSION:Keeping in view that 8%-18% of donor population in India is anti-HBc reactive,inclusion of anti-HBc testing will lead to high discard rate.Anti-HBs as proposed previously does not seem to predict clearance of the virus.Cost effectiveness of introducing universal anti-HBc screening and discarding large number of blood units versus considering ID NAT (Individual donor nuclic acid testing) needs to be assessed.

  1. Prognosis of chronic hepatitis B transmitted from HBsAg positive mothers.

    OpenAIRE

    Bortolotti, F; Cadrobbi, P; Armigliato, M; Rude, L; Rugge, M; Realdi, G

    1987-01-01

    Nine children born to HBsAg positive mothers, who became chronic HBsAg carriers with associated liver disease, were followed for five to 10 years. Five children with active hepatitis or active cirrhosis at presentation achieved complete remission within six years, while three HBeAg positive patients with minimal histological lesions remained unchanged.

  2. Low serum hyaluronic acid levels associated with spontaneous HBsAg clearance

    NARCIS (Netherlands)

    Harkisoen, S.; Arends, J. E.; van den Hoek, A.; van Erpecum, K. J.; Boland, G. J.; Hoepelman, A. I. M.

    2015-01-01

    Purpose The pathophysiological underlying mechanism of spontaneous HBsAg clearance in hepatitis B virus (HBV) infected patients is largely unknown. However, serum hyaluronic acid (sHA) plays a role in liver fibrosis progression and reversely could serve as a potential biomarker for HBsAg clearance.

  3. High Prevalence of HBsAg during Pregnancy in Asian Communities at Cayenne Hospital, French Guiana

    OpenAIRE

    Mahamat, Aba; Louvel, Dominique; Vaz, Tania; Demar, Magalie; Nacher, Mathieu; Djossou, Félix

    2010-01-01

    We described hepatitis B surface antigen (HBsAg) prevalence among 2,347 pregnant women having delivered at the Cayenne hospital in 2007 according to ethnicity. With 11.0% HBsAg prevalence, Asian women (Hmong and Chinese) were the group with the highest risk of hepatitis B virus (HBV) perinatal transmission compared with other ethnic groups.

  4. Seroprevalence of hepatitis B e antigen (HBe antigen) and B core antibodies (IgG anti-HBcore and IgM anti-HBcore) among hepatitis B surface antigen positive blood donors at a Tertiary Centre in Nigeria

    OpenAIRE

    Akinbami Akinsegun A; Oshinaike Olajumoke O; Dosunmu Owolabi A; Adeyemo Titilope A; Adediran Adewumi; Akanmu Sulaiman; Wright Kikelomo O; Ilori Seun; Aile Kinsley

    2012-01-01

    Abstract Background Hepatitis B virus (HBV) is a common cause of liver disease throughout the world. HBV is transmitted through blood and other body fluids, including semen and saliva. Chronic replication of HBV virons is characterized by persistence circulation of HBsAg, HBeAg and HBV DNA; usually with anti-HBc and occasionally with anti-HBs. Aim: To determine the prevalence of HBeAg, IgG anti-HBcore and IgM anti-HBcore amongst HBsAg positive blood donors. These parameters are reflective of ...

  5. Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines.

    Science.gov (United States)

    Wu, Yajun; Li, Shuai; Li, Haixia; Zhao, Chunzhi; Ma, Hao; Zhao, Xiunan; Wu, Junhua; Liu, Kunlu; Shan, Junjie; Wang, Yuxia

    2016-10-01

    Poria cocos has a long history of medicinal use in China. Polysaccharides and their derivatives in the medicine exhibit many beneficial biological activities including anticancer, anti-inflammatory, antioxidant and antiviral activities. In this study, a new polysaccharide (PCP-II) was isolated from sclerotium of Poria cocos. Its physico-chemical characters were identified and its adjuvant activity was investigated in mice co-immunized with H1N1 influenza vaccine and hepatitis B surface antigen (HBsAg). The results revealed that PCP-II has a molecular weight of 29.0kDa. It was composed of fucose, mannose, glucose and galactose in molar ration of 1.00:1.63:0.16:6.29 respectively. Pharmacological data demonstrated that PCP-II increased antigen-specific antibody levels in mice immunized with influenza vaccine. PCP-II also elicited anti-HBsAg antibodies at significantly higher titers and generated robust and durable immunity compared to mice immunized with HBsAg-alum following two administrations. PCP-II improved proliferation of splenocytes, stimulated IL-12p70 and TNF-α productions in dendritic cells and macrophages respectively. These results suggested that PCP-II-adjuvanted vaccines enhanced humoral and cellular immunity. PCP-II could be developed as an efficacious adjuvant in human and animal vaccines. PMID:27185068

  6. Estudo da soroprevalência do AgHBs em gestantes da 15ª Regional de Saúde e da imunoprofilaxia para os recém-nascidos das gestantes AgHBs positivo = Study into the HBsAg seroprevalence in pregnant women from the 15th Health Regional and the immunoprophylaxia on the newborns of these HBsAg-positive women

    Directory of Open Access Journals (Sweden)

    Sonia Kaori Miyamoto

    2008-01-01

    Full Text Available Determinar a prevalência do AgHBs nas gestantes da 15ª Regional de Saúde (15ª R.S. atendidas no Laboratório de Ensino e Pesquisa em Análises Clínicas (Lepac, Universidade Estadual de Maringá, e verificar se foi solicitada a imunoprofilaxia para hepatite B aos recém-natos das gestantes AgHBs positivo, no período de janeiro de 1998 a dezembro de 2002. A pesquisa do AgHBs foi realizada pela técnica imunoenzimática IMxHBsAg e Axsym HBsAg (Laboratório Abbott. As solicitações de imunobiológicos especiais para a imunoprofilaxia da hepatite B ao CRIE foram obtidas na Seção de Epidemiologia da 15ª R.S. Foram analisadas 12.274 gestantes e a prevalência do AgHBs foide 1,0%. Dentre as 125 gestantes AgHBs positivo, foram solicitadas imunoprofilaxia para 32 (25,6% recém-nascidos. Ainda que a prevalência encontrada indique ser esta uma área de baixa endemicidade, os resultados reafirmam a importância da realização do diagnóstico da hepatite B no atendimento pré-natal, para adoção da imunoprofilaxia no recém-nascido.To determine HBsAg prevalence among pregnant women from the 15th Health Regional assisted in the Laboratório de Ensino e Pesquisa em Análises Clínicas (Lepac, Universidade Estadual de Maringá, and verify whether immunoprophylaxis with vaccine and immunoglobulin was requested for the newborns of HBsAg-positive women during the period from January 1998 to December 2002. The research about HBsAg was conducted using the immunoenzymatic IMxHBsAg (Abbot Lab and Axsym HBsAg (Abbot Lab techniques. The requests to the CRIE for specialimmunobiologic agents for Hepatitis B immunoprophylaxis were obtained in the Department of Epidemiology from the 15th Health Regional. The analysis includes 12,274 pregnant women, and the HBsAg prevalence was 1.0%. Among 125 HBsAg positive pregnant women, immunoprophylaxis was requested for 32 (25.6% newborns. Although the prevalence detected demonstrates this to be a low endemic area, the

  7. HBsAg seroconversion after pegylated interferon alfa 2a rescue in a lamivudine-resistant patient with HBeAg-negative chronic hepatitis B and favourable IL28-B genotype.

    Science.gov (United States)

    Stanzione, Maria; Stornaiuolo, Gianfranca; Rizzo, Viviana; Pontarelli, Agostina; Gaeta, Giovanni Battista

    2016-06-01

    Hepatitis B virus (HBV) surface antigen (HBsAg) seroconversion to anti-HBs antibody is the best final objective for all available chronic hepatitis B (CHB) treatments. Unfortunately, this goal is rarely achieved with the currently applied therapeutic approaches. Here we describe the case of an anti-HBe-positive CHB patient who was successfully treated with a particular therapeutic schedule. The patient was initially treated with lamivudine (LAM) for nine years. Breakthrough was observed after eight years of LAM therapy. HBV-DNA was 3x10E4 IU/mL and LAM resistance mutations were present. Subcutaneous pegylated interferon (PEG-IFN) alfa 2a, 180 mcg/week, was added to LAM and after 4 weeks LAM was discontinued and PEG-IFN alone was continued up to week 52. HBV-DNA became undetectable at week 4 of therapy; serum HBsAg started to decline from week 4 and became undetectable at week 36, with the subsequent appearance of anti-HBs antibodies. IL28-B was genotyped at the polymorphic site rs12979860 and the CC allele was detected. Rescue therapy with Peg-IFN may be an option for selected patients with resistance to nucleos(t)ide analogues. PMID:27367326

  8. Kinetics of serum HBsAg in Chinese patients with chronic HBV infection with long-term adefovir dipivoxil treatment

    Institute of Scientific and Technical Information of China (English)

    Li Minran; Xi Hongli; Wang Qinhuan; Hou Fengqin; Huo Na; Zhang Xiaxia; Li Fang

    2014-01-01

    Background Knowledge on Hepatitis B surface antigen (HBsAg) kinetics in chronic hepatitis B (CHB) patients with longterm adefovir dipivoxil (ADV) treatment is limited.The aims of this study were to investigate HBsAg kinetics in patients with chronic hepatitis B virus (HBV) infection treated with long-term ADV and to evaluate different characteristics between patients with and without HBsAg loss.Methods We retrospectively evaluated HBsAg kinetics in 24 Chinese patients with chronic HBV infection who achieved continuous virologic suppression during ADV therapy.HBV genotype was determined at baseline.Liver biochemistry,hepatitis B e antigen status,serum HBV DNA,and HBsAg levels were measured at baseline,6 months,and once every year thereafter.Results Of these 24 patients,3,1,and 20 patients were followed up for 3,5,and 6 years,respectively.Baseline serum HBsAg level had a moderate correlation with baseline HBV DNA level (r=0.52,P=0.01).The median rate of HBsAg reduction during the therapy period was 0.08 Ig IU·ml-1·y-1.Baseline serum HBsAg level was significantly higher than other time points (P ranges from 0.046 to 0.002).The HBsAg reduction rate during the first year was similar to that in other years (P>0.05).The HBsAg reduction rate during the first year in patients with eventual HBsAg loss was significantly faster than that in patients without HBsAg loss (P=0.005).Conclusions Serum HBsAg levels in Chinese CHB patients receiving long-term ADV demonstrated a gradual reduction.Patients with eventual HBsAg loss had a significantly faster HBsAg reduction rate during the first year than those without HBsAg loss.

  9. Deteksi HBsAg dan HBeAg dalam Saliva Pengidap Virus Hepatitis B

    Directory of Open Access Journals (Sweden)

    Riemawati A. Lesmana

    2015-10-01

    Full Text Available Transmission of hepatitis B virus (HBV via blood or its product has been well established. However, body fluids like urine and saliva may also contain HBV. A complete HBV consists of HBsAg, HBeAg, HBcAg dan partikel DNA. Hepatitis B carrier is detected by the presence of serologic marker HBsAg while the ongoing viral replication or infectivity is diagnosed by the presence of HBeAg or DNA particle. Meanwhile dentists will often contact with the saliva of their patients in daily practice. This cross-sectional study was carried out to assess the infectivity of the saliva of HBV carriers. During a 10 month period (August 1994 - May 1995 detection of HBsAg and HBeAg in blood and saliva were done in 97 HBV carriers using the ELISA method (Enzyme Linked Immunosorbent Assay. Of 97 HBV carriers both positive gor HBsAG in blood were found 56 (Group I and positive HBsAg and negativa HBeAg in the other 41 (Group II. Examination of the saliva of HBV carriers in Group I showed positive HBsAg as well as HBeAg in 48 (85,7%, only positive for HBsAg in 5 (10,7% and both negative for HBsAg and HBeAg in the other 2 (3,6% where as in Group II positive for both HBsAg and HBeAG in the remaining 10 (24,4%. In conclusion, the majority of highly infectious hepatitis B carriers do also have infectious saliva which could be an important source of infection and transmission of the virus in the field of dentistry.

  10. Research Progress in Purification Technology of Genetically Engineered HBsAg%基因工程HBsAg纯化技术的研究进展

    Institute of Scientific and Technical Information of China (English)

    潘太健; 马瑞; 曹春来; 彭韪; 孙万邦; 肖拥军

    2012-01-01

    乙型肝炎病毒表面抗原(HBsAg)是乙肝疫苗(hepatitis B vaccine)的重要组成部分,注射乙肝疫苗是目前预防乙型肝炎感染最有效的方法.血源性乙肝疫苗因成本、来源和潜在其他病毒的感染已被淘汰,取而代之的是基因工程技术生产的第2代重组乙肝疫苗.主要介绍了国内外重组HBsAg在样品净化捕获、中度纯化和精纯3个阶段的主流纯化技术.%Hepatitis B surface antigen(HBsAg) is the important component of hepatitis B vaccine and injecting hepatitis B vaccine is the most effective way to prevent hepatitis B. As a result of high costs,limited sources and potential infection,the plasma - derived hepatitis B vaccine is replaced by the second generation of recombinant HBsAg produced by genetic engineering technology. This paper summarizes the mainstream purification technology during 3 stages of sample purification capture, intermediate purification and fine purification of recombinant HBsAg at home and abroad.

  11. Construction and immunological evaluation of truncated hepatitis B core particles carrying HBsAg amino acids 119-152 in the major immunodominant region (MIR).

    Science.gov (United States)

    Su, Qiudong; Yi, Yao; Guo, Minzhuo; Qiu, Feng; Jia, Zhiyuan; Lu, Xuexin; Meng, Qingling; Bi, Shengli

    2013-09-13

    Hepatitis B capsid protein expressed in Escherichia coli can reassemble into icosahedral particles, which could strongly enhance the immunogenicity of foreign epitopes, especially those inserted into its major immunodominant region. Herein, we inserted the entire 'α' antigenic determinant amino acids (aa) 119-152 of HBsAg into the truncated HBc (aa 1-144), between Asp(78) and Pro(79). Prokaryotic expression showed that the mosaic HBc was mainly in the form of inclusion bodies. After denaturation with urea, it was dialyzed progressively for protein renaturation. We observed that before and after renaturation, mosaic HBc was antigenic as determined by HBsAg ELISA and a lot of viruslike particles were observed after renaturation. Thus, we further purified the mosaic viruslike particles by (NH4)2SO4 precipitation, DEAE chromatography, and Sepharose 4FF chromatography. Negative staining electron microscopy demonstrated the morphology of the viruslike particles. Immunization of Balb/c mice with mosaic particles induced the production of anti-HBs antibody and Th1 cell immune response supported by ELISPOT and CD4/CD8 proportions assay. In conclusion, we constructed mosaic hepatitis core particles displaying the entire 'α' antigenic determinant on the surface and laid a foundation for researching therapeutic hepatits B vaccines. PMID:23969156

  12. [A simple ELISA method for the detection of HBsAg: Organon Teknika HBsAg Uniform II screening and confirmation test. Comparative study using the HBsAg Hapanostika method. A multicenter study].

    Science.gov (United States)

    Pár, A; Mihály, I; Kömives, K

    1994-09-25

    An one-step enzyme-linked immunoabsorbent method, named as HBsAg Uniform II has been described for the detection of serum HBsAg, and a comparison was made with a widely used ELISA technique HBsAg Hepanostika test, to evaluate sensitivity, specificity and reproducibility of the method. A total of 531 serum samples from patients with liver disease and with renal failure, as well as 1065 samples from blood donors have been investigated. While the sensitivity of Uniform II vs. Hepanostika was 99.5% vs. 72.7%, the specificity was 99.2% of both methods. The positive predictive values did not differ (99.5% vs. 99.2%), however, the negative predictive values were 99.2% vs. 71.7%, respectively, in favour of Uniform II test. The Uniform II confirmatory test confirmed the positive HBsAg results in 94%, this rate was 74% using Hepanostika system. The new method proved to be a simple, quick, reliable test, which can be useful as a valuable tool in both the clinical diagnosis and blood donor screening.

  13. HBsAg, HBcAg, and combined HBsAg/HBcAg-based therapeutic vaccines in treating chronic hepatitis B virus infection

    Institute of Scientific and Technical Information of China (English)

    Sheikh Mohammad Fazle Akbar; Mamun Al-Mahtab

    2013-01-01

    BACKGROUND: As  the  host  immunity  is  diminished  in patients with chronic hepatitis B (CHB), different approaches have  been  used  to  up-regulate  their  immune  responses  to produce therapeutic effects. But, cytokines, growth factors and polyclonal  immune  modulators  could  not  exhibit  sufficient therapeutic  effects  in  these  patients.  Immune  therapy  with HBV-related antigens (vaccine therapy) has been used in CHB patients. But there is a paucity of information about the design of HBV antigen-based immune therapy in these patients. DATA  SOURCE: Preclinical  and  clinical  studies  on  immune therapy with HBsAg-based vaccine, HBcAg and combination of HBsAg/HBcAg-based vaccines have been discussed. RESULTS: HBsAg-based  prophylactic  vaccine  was  used  as an  immune  therapeutic  agent  in  CHB  patients;  however, monotherapy  with  HBsAg-based  immune  therapy  could not  lead  to  sustained  control  of  HBV  replication  and/or  liver damages.  HBsAg-based  vaccine  was  used  as  a  combination therapy  with  cytokines,  growth  factors,  and  antiviral  drugs. HBsAg-based  vaccine  was  also  used  for  cell-based  therapy. However,  satisfactory  therapeutic  effects  of  HBsAg-based vaccine could not be documented in CHB patients. In the mean time, evidences have supported that HBcAg-specific immunity is  endowed  with  antiviral  and  liver  protecting  capacities  in CHB  patients.  Recent  data  concentrate  on  the  clinical  use  of combined HBsAg- and HBcAg-based vaccines in CHB patients. CONCLUSION: Antigen-based  immune  therapy

  14. Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan

    Institute of Scientific and Technical Information of China (English)

    Ismail Jalil; Muhammad Arshad; Zara Rafaque; Fazle Raziq; Robina Wazir; Sajid Malik; Javid Iqbal Dasti

    2016-01-01

    Objective: To study the seroprevalence of hepatitis B virus (HBV) and hepatitis delta virus (HDV) infections in patients visiting outpatient department of a major tertiary care hospital in Khyber Pakhtunkhwa region of Pakistan. Methods: Blood samples were collected from non-hospitalized patients. Serological analysis was done by ELISA and viral DNA was amplified by PCR. The amplified DNA was analyzed by agarose gel electrophoresis. Results: Altogether, 946 blood samples were screened, overall percentage of HBsAg-positive patients remained 22.41%(prevalence:224.10/1 000;CI:0.197 5 ± 0.250 7) with the highest incidence rates among relatively younger age groups (20–29 years). The prevalence of HBV–HDV co-infection was found to be 46.75/1 000;CI:0.031 8 ± 0.061 7. In HBsAg-positive patients, anti-HBc-total was detected in 86.79%while 25.00% were positive for anti-HBc-immunoglobulin M. Similarly, among these patients, HBV DNA was detected in 64.13% and 10.85% were co-infected with HDV. Different symptoms were associated with the prevailing infection, including malaise (62%), anorexia (66%) and fa-tigue (73%). The most commonly associated symptom was abdominal discomfort. Among these patients, certain risk factors, including surgery, visit to dentist and intravenus infusions were frequently associated with the infection (c2=95.23;df=11;P<0.000 1). Conclusions: Overall, this study confirmed higher prevalence of active HBV/HDV infection, among young patients from Khyber Pakhtunkhwa region having no prior history of viral hepatitis.

  15. Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales, Phaeophyta)

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A transformation model for Laminaria japonica was established from 1993 to 1998, on the basis of which the transgenic kelp with heterologous gene encoding hepatitis B surface antigen (HBsAg) was obtained by using the micro- particle bombardment transformation method. Results of quantitative ELISA showed that HBsAg in transgenic kelp was 0.529 μg/mg soluble proteins on average and the highest value was 2.497 μg/mg, implying that recombinant HBsAg had natural epitope. Further support for the integration of HBsAg gene into kelp genome was obtained by PCR- Southern and total DNA hybridization. Prospect of kelp bioreactor producing high value materials such as edible HBV vaccine was discussed as well.

  16. Comparative study of methods of detection of hepatitis ′B′ surface antigen (HBsAg.

    Directory of Open Access Journals (Sweden)

    Parab V

    1989-04-01

    Full Text Available The serum samples were collected from 52 patients of acute viral hepatitis and 235 hospital staff from Kasturba Hospital for Infectious Diseases. HBsAg was detected in their sera by counter-immuno-electrophoresis (CIEP, reverse passive hemogglutination (RPHA and by micro-enzyme-linked-immunosorbent assay (ELISA technique. Among the patients, HBsAg was detected in 12 cases (23% by CIEP, in 18 cases (34% by RPHA and in 23 patients (45% by ELISA. In the hospital staff, HBsAg was detected in 4 samples (1.7% by CIEP, in 8 samples (3.5% by RPHA and in 32 samples (13.5% by ELISA. Thus ELISA was found to be the most sensitive technique in detecting HBsAg.

  17. Clinical Significance of Establishing Relationship between the HBsAg Test and HBV DNA%乙肝五项测定结果与HBV DNA的关系及其临床意义

    Institute of Scientific and Technical Information of China (English)

    李昕; 张荣波

    2011-01-01

    Objective To explore the internal relationship and the clinical significance of HBVM( HBsAg, Anti-HBs,HBeAg,Anti-HBe, Anti-HBc )and HBV DNA( Hepatitis B Virus DNA ). Methods HBVM and HBV-DNA of 300 HBV patients were examined by enzyme linked immunosorbent assay( ELISA )and quantitative fluorescence PCR technique respectively. Results Of all the clinical results of 300 HBV patients,110 patients showed HBsAg( + ),HBeAg( + ),HBcAb( + )( HBV DNA positive rate 99. 1% ),85 patients showing HBsAg( + ), HBeAb( + ),HBcAb( + )( HBV DNA positive rate 69.4% ), 39 patients showing HBsAg( + ),HBcAb( + )( HBV DNA positive rate 53.8% ),53 patients showing HBeAb( + ),HBcAb( + )( HBV DNA positive rate 35.8% ). HBV DNA positive rate of HBsAg( + ),HBeAg( + ),HBcAb( + ) and HBsAg( + ), HBeAb( + ), HBcAb( + ) were different significantly( x2 = 35. 406, P < 0.05 ). Conclusion The results of HBsAg and HBV-DNA are closely related with different clinical meanings,which makes it necessary to combine the two results for a correct diagnosis and treatment.%目的探讨乙型肝炎(乙肝)五项结果和HBV DNA检出情况间的关系和临床意义.方法运用聚合酶链反应法检测300例乙肝血清的HBV DNA,并用酶联免疫吸附实验法进行乙肝五项的测定,对结果进行比较分析.结果 300例乙肝门诊的患者中乙肝五项的结果为110例HBsAg(+)、HBeAg(+)、HBcAb(+)(大三阳),其HBV DNA的阳性率为99.1%,85例HBsAg(+)、HBeAb(+)、HBcAb(+)(小三阳),其HBV DNA的阳性率为69.4%,39例HBsAg(+)、HBcAb(+),其HBV DNA的阳性率为53.8%,53例HBeAb(+)、HBcAb(+),其HBV DNA的阳性率为35.8%.大三阳和小三阳的HBV DNA阳性率比较差异有统计学意义(χ2=35.406,P<0.05).结论乙肝五项的结果与HBV DNA的结果有着密切的联系,并且都具备各自的临床意义,因此两者必须结合才能正确地对乙肝患者的病情作出正确分析和判断.

  18. Evaluación de la respuesta de anticuerpos hacia antígenos de Pseudomonas aeruginosa.

    Directory of Open Access Journals (Sweden)

    Aniel Moya

    2007-04-01

    Full Text Available Pseudomonas aeruginosa es un patógeno extracelular que genera una respuesta de anticuerpos específicos con utilidad para el diagnóstico y vacunas. En el presente estudio nos propusimos evaluar en suero humano los niveles de anticuerpos contra antígenos relevantes de P. aeruginosa. Realizamos la determinación de anticuerpos IgG contra tres exoenzimas, consideradas como factores de virulencia de mayor importancia en infecciones. Este resultado dio paso a la evaluación del reconocimiento de IgG e IgA hacia antígenos de la envoltura celular bacteriana por ELISA de células enteras. Todos los sueros evaluados mostraron títulos de IgG e IgA superiores a los individuos sanos, con excepción de dos muestras de pacientes que no mostraron alto título. Este ensayo permitió analizar el nivel de reconocimiento hacia los antígenos más expuestos de la bacteria que incluyen principalmente LPS y proteínas de membrana externa. Se encontró diferencias entre los valores de densidad óptica a 450 nm de individuos sanos y enfermos. El método usado permitió seleccionar dos sueros de pacientes de diferentes tipos de infecciones que fueron comparados por Western blot. Se observó que aunque los sueros tenían reacción hacia distintos serotipos de P. aeruginosa, la intensidad del reconocimiento variaba según el tipo de infección.

  19. Encefalitis por anticuerpos contra el receptor de NMDA: experiencia con seis pacientes pediátricos. Potencial eficacia del metotrexato

    Science.gov (United States)

    Bravo-Oro, Antonio; Abud-Mendoza, Carlos; Quezada-Corona, Arturo; Dalmau, Josep; Campos-Guevara, Verónica

    2016-01-01

    Introducción La encefalitis por anticuerpos contra el receptor de N-metil-D-aspartato (NMDA) es una entidad cada vez más diagnosticada en edad pediátrica. A diferencia de los adultos, en muchos casos no se asocia a tumores y las manifestaciones iniciales en niños más frecuentes son crisis convulsivas y trastornos del movimiento, mientras que en los adultos predominan las alteraciones psiquiátricas. Casos clínicos Presentamos seis casos pediátricos confirmados con anticuerpos contra la subunidad NR1 del receptor de NMDA en suero y líquido cefalorraquídeo. Cinco de los casos comenzaron con crisis convulsivas como manifestación clínica inicial antes de desarrollar el cuadro clásico de esta entidad. En todos los casos se utilizaron esteroides como primera línea de tratamiento, con los que sólo se observó control de las manifestaciones en uno, por lo que el resto de los pacientes requirió inmunomoduladores de segunda línea. Todos los pacientes recibieron metotrexato como tratamiento inmunomodulador para evitar recaídas y la evolución fue a la mejoría en todos ellos. Conclusiones En nuestra serie de pacientes con encefalitis por anticuerpos contra el receptor de NMDA, ninguno se asoció a tumores. Todos los casos recibieron metotrexato por lo menos durante un año, no observamos eventos adversos clínicos ni por laboratorio, ni hubo secuelas neurológicas ni recaídas durante el tratamiento. Aunque es una serie pequeña y es deseable incrementar el número y tiempo de evolución, consideramos el metotrexato una excelente alternativa como tratamiento inmunomodulador para esta patología. PMID:24150952

  20. DNA-mediated immunization of mice with plasmid encoding HBs antigen.

    OpenAIRE

    Yoon, S. J.; Park, J W; Ahn, S. Y.; Choe, B. K.; Suh, M. H.

    1999-01-01

    In order to develop an experimental DNA vaccine for the prevention and treatment of hepatitis B virus infection, hepatitis B virus surface antigen (HBsAg) DNA was subcloned into an E. coli-eukaryotic cell shuttle vector and was expressed in the Baculovirus expression system. Intramuscular, intradermal, and intraperitoneal injections of 30 microg of the plasmid DNA expressing HBsAg induced humoral and cellular immune responses in ICR mice. The first IgG antibodies were detected after ten days ...

  1. Prevalencia de anticuerpos contra Brucella sp. En donantes del banco de sangre de un hospital de Lima

    OpenAIRE

    Abel Ortega; José Paredes; Alfredo Guillén

    2007-01-01

    El Perú es un país endémico de Brucelosis, por lo que existe el riesgo de transmisión a través de sangre donada por no ser parte del tamizaje regular que realizan los bancos de sangre. Para conocer la prevalencia de anticuerpos contra Brucella en donantes del banco de sangre del Hospital Edgardo Rebagliati Martins, se analizó 1003 muestras de suero. La prueba tamiz fue Rosa de Bengala (RB); las muestras positivas fueron evaluadas por las pruebas de aglutinación en tubo (AT) y 2-Mercaptoetanol...

  2. Determination of HBsAg subtypes in Western Siberian part of Russia.

    Science.gov (United States)

    Netesova, I G; Swenson, P D; Osipova, L P; Gubina, M A; Posukh, O L; Netesov, S V

    2003-10-01

    A set of monoclonal antibodies with specificity for hepatitis B surface antigen (HBsAg) was used for subtyping this antigen in sera from indigenous natives, blood donors, and drug users in Western Siberia with a modified commercial enzyme immunoassay kit for HBsAg detection. Three subtypes of HBsAg in a ratio of 36 (78%) ayw2:8 ayw3varB (18%):2 (4%) adw2 were found in 46 (100%) HBsAg-positive sera of different aboriginal populations of Western Siberia: the Tundra Nenets, Northern Khanty, Southern Altaians, and Kazakhs. Four subtypes of HBsAg in a ratio of 81 (57%) ayw2:58 (15 ayw3varA and 43 ayw3varB; 44%):2 (1%) adw2 were detected in 141 (100%) samples of blood donors from ten cities of Western Siberia. Three subtypes of HBsAg in a ratio of 34 ayw3:(both variants, 33 ayw3varA and 1 ayw3varB; 97.1%):1 (2.9%) ayw2 were found in blood of 35 injection drug users in Novosibirsk.

  3. Producción de un anticuerpo IgY específico contra el antígeno CD41 humano

    Directory of Open Access Journals (Sweden)

    Esteban J. Gutiérrez Calzado

    2009-01-01

    Full Text Available Desde hace dos décadas, la comunidad científica muestra un gran interés hacia la generación de anticuerpos en gallinas ponedoras por las ventajas que estas aportan al compararse con otros métodos tradicionales para este fin, sobre todo, en el hecho de lograrse en ellas, mejores respuestas de anticuerpos hacia antígenos de mamíferos muy conservados en la filogenia entre los que se encuentran glicoproteínas de membrana celular. El objetivo de este trabajo fue la generación de anticuerpos específicos contra la glicoproteína de membrana plaquetaria humana gpIIb/IIIa (antígeno CD41 en gallinas ponedoras. Para este propósito, se utilizaron gallinas de 22 semanas de edad, a las que se les aplicaron dosis de 20 ¿g de una preparación inmunopurificada de este antígeno plaquetario, emulsionada con adyuvante completo e incompleto de Freund para una primera y segunda inmunización respectivamente, a intervalos de un mes. Se cosecharon los huevos diariamente de los animales inmunizados a partir de un día antes de esta aplicación hasta 30 d posteriores a la segunda inyección, se procesaron estos para la extracción de los anticuerpos específicos presentes en ellos y se obtuvo la dinámica de producción de anticuerpos por el método clásico de Ouchterlony que se enfrentó a una preparación pura del antígeno. Las respuestas de anticuerpos obtenidas resultaron en títulos adecuados susceptibles de investigar en usos posteriores en la Medicina Transfusional específicamente para el análisis de la calidad de plasmas.

  4. POLYCLONAL ANTIBODIES TO DETECT THE CP-RT PROTEIN OF Potato Mop-Top VIRUS ANTICUERPOS POLICLONALES PARA LA DETECCIÓN DE LA PROTEÍNA CP-RT DEL Potato Mop-Top VIRUS

    Directory of Open Access Journals (Sweden)

    Derly Andrade Molina

    2012-06-01

    Full Text Available Abstract. Potato mop-top virus (PMTV causes an important re-emerging disease in potato crops in Colombia due to the increased incidence of its protozoan vector Spongospora subterranea f.sp. subterranea (Sss, the causing agent of Powdery scab disease. For an accurate detection of PMTV it is recommended to combine different diagnostic tests and evaluate multiple samples per plant or tissue. In order to increase the number of available tools for detection of PMTV, antibodies targeting the RT domain of the CP-RT protein were developed in this work. Sequencing of the RT domain from the colombian strain R25 was achieved and using bioinformatic analysis, a potential antigenic region was identified. A peptide mimicking the antigenic region was inoculated in rabbits for the production of polyclonal antibodies. The antibodies were tested by ELISA using Nicotiana benthamiana bait-plants infected with Sss cystosori and potato plants collected in La Unión (Antioquia. The validity of serological tests was confirmed by RT-PCR. A complete sequence of the RT domain and 441 nt of the CP gene were obtained. Phylogenetic analysis identified strain R25 as closely associated to the PMTV lineage distributed worldwide. A total of 19.26 mg of anti-CP-RT polyclonal antibodies useful in detecting PMTV in infected plants were obtained. As CP-RT is involved in transmission of PMTV by Sss, these antibodies will be useful for supporting not only diagnostic programs but also basic and epidemiologic studies aimed at understanding interactions between PMTV and Sss.Resumen. El Potato mop-top virus (PMTV es uno de los virus re-emergentes en los cultivos de papa de Colombia, como resultado del aumento de la incidencia de su vector natural, el protozoo Spongospora subterranea f.sp. mamarinm@unal.edu.co (Sss, agente causal de la Sarna polvosa de la papa. Para la detección del virus se recomienda la realización simultánea de diferentes pruebas, así como la evaluación de m

  5. Utilidad de las muestras de sangre total desecada en papel de filtro en la detección de anticuerpos antitoxoplasma

    Directory of Open Access Journals (Sweden)

    Joaquim Gascon

    1989-04-01

    Full Text Available Se compara la técnica de Aglutinación Directa (AD utilizando muestras de sangre total desecada en papel de filtro, con la técnica de ELISA y la misma AD utilizando muestras de suero de los mismos pacientes, para la detección de anticuerpos antitoxoplasma. Los resultados muestran la validez del método de la sangre desecada en papel de filtro para la detección de anticuerpos antitoxoplasma con la técnica de AD, y se considera su utilidad en los estudios epidemiológicos de campo.

  6. Prevalencia de anticuerpos IgG al toxoide tetánico en trabajadores sanitarios

    Directory of Open Access Journals (Sweden)

    B. Pérez Bermúdez

    2014-09-01

    Full Text Available Introducción: En el año 2009 el Ministerio de Sanidad y Consumo de España publica una nueva guía para la vacunación de la Difteria y Tétanos. Existen discrepancias con los tiempos de inoculación de las dosis de recuerdo con otros países y sociedades médicas. Material y métodos: Estudio descriptivo. Las variables analizadas fueron: IgG-Toxoide Tetánico, tiempo última dosis de vacunación, dosis totales, edad, sexo, hospital, habito tabáquico e índice de masa corporal. Se construyó un modelo de Regresión Logística. Con el fin de determinar los puntos de corte más sensibles y específicos para las variables temporales (tiempo desde la última dosis y edad se calcularon las curvas R.O.C Resultados: Las variables, edad O,R= 17,56 (1,840 - 167,760, dosis de vacuna inoculadas al trabajador, O,R= 0,064 (0,006 - 0,908 y tiempo de la última dosis de vacunación, O,R= 1,03 (1,017 - 1,047, son las variables significativas en el análisis multivariante. Los puntos de cortes, para la edad son 54 años (Sensibilidad S= 0,72, Especificidad SP=0,77 y 15 años tras la última dosis años (S= 0,88, SP=0,86, por encima de este corte la probabilidad de no tener anticuerpos al Tétanos es alta. Conclusiones: Ante un trabajador que refiere o documenta que hace más de 15 años de la ultima dosis deberíamos plantearnos la revacunación, si además este trabajador refiere no tener más de 5 dosis inoculadas y tiene más de 54 años la revacunación sería muy recomendable.

  7. Anticuerpos monoclonales contra la Gonadotropina Corionica Humana (HCG y su empleo en el diagnóstico precoz del embarazo.

    Directory of Open Access Journals (Sweden)

    Torres E

    2006-03-01

    Full Text Available Se describe la obtención y caracterización de anticuerpos monoclonales contra la hormona gonadotropina cariónica humana (HCG, seleccionados de la inmunización subcutánea en ratones BALB/c. Se reporta también el desarrollo del sistema rápido HeberFast Line® Embarazo, para la detección de la hormona en muestras de orina, basado en los anticuerpos obtenidos y la tecnología del flujo lateral desarrollada en membranas de nitrocelulosa como soporte sólido. El nivel de sensibilidad alcanzado con este sistema diagnóstico es de 20 UI/L de HCG en muestra de orina, y no se observó reactividad cruzada en analitos similares conteniendo concentraciones de las glicohormonas relacionadas; 1000 IU de LH, 5000 IU de FSH, y 1000 µIU de TSH respectivamente. Los estudios de estabilidad arrojaron, que el diagnosticador mantine las características de funcionalidad por un tiempo de 15 meses a temperaturas entre 2 y 30 ºC, conformando este el tiempo de vigencia del sistema. De poseer en Farmacias, Consultorios Médicos de la Familia y Hospital Gineco-Obstétricos, un sistema rápido y sencillo como el establecido en este trabajo, se pueden evitar todos los riesgos e inconvenientes que acarrean los procesos de interrupción del embarazo en la mujer.

  8. Prevalencia de anticuerpos contra Brucella sp. En donantes del banco de sangre de un hospital de Lima

    Directory of Open Access Journals (Sweden)

    Abel Ortega

    2007-10-01

    Full Text Available El Perú es un país endémico de Brucelosis, por lo que existe el riesgo de transmisión a través de sangre donada por no ser parte del tamizaje regular que realizan los bancos de sangre. Para conocer la prevalencia de anticuerpos contra Brucella en donantes del banco de sangre del Hospital Edgardo Rebagliati Martins, se analizó 1003 muestras de suero. La prueba tamiz fue Rosa de Bengala (RB; las muestras positivas fueron evaluadas por las pruebas de aglutinación en tubo (AT y 2-Mercaptoetanol (2-ME. Dos donantes fueron positivos a RB, confirmados por AT y 2-ME, se encontró una prevalencia de 0,20% (IC99%: 0,01-0,92. Se demostró la presencia de donantes portadores de anticuerpos contra Brucella con posible Brucelosis activa, evidenciándose la posibilidad de transmisión de esta infección. Se debe realizar mayores estudios con la finalidad de conocer la realidad de otros bancos de sangre.

  9. 污染HBsAg的口腔器械消毒方法的实验研究%Experimental study on sterilizing methods of the dental instruments contaminated with HBsAg

    Institute of Scientific and Technical Information of China (English)

    杨聚才; 安银东; 黄萍; 王军玲

    1999-01-01

    目的:探讨一种能在较短时间内灭活HBV的消毒方法.方法:采用不同浓度的戊二醛擦拭消毒已污染HBsAg阳性血清的口腔专科常用诊疗器械,用ELISA法检测HBsAg.结果:用20~30ml/L戊二醛消毒剂擦拭消毒1遍已污染HBsAg的口腔器械,HBsAg的灭活率为8%,而采用擦拭消毒3遍的方法,HBsAg的灭活率为92%~100%.结论:选用较高浓度的戊二醛消毒剂擦拭消毒3遍,可以达到灭活HBsAg的目的.

  10. Avaliação eosinofílica e soropositividade para anticorpos IgG anti-toxocara em crianças atendidas pelo Sistema Único de Saúde Evaluación eosinófila y suero-positividad para anticuerpos igG anti-toxocara en niños atendidos por el Sistema Único de Salud Eosinophilic count and seropositivity for IgG antibodies to toxocara spp. in children assisted at the public health service

    OpenAIRE

    Ariella Andrade Marchioro; Cristiane Maria Colli; Salete Mattia; Márcia Liz Paludo; Gisely Cardoso de Melo; Carolina Moreira Adami; Sandra Marisa Pelloso; Ana Lúcia F. Guilherme

    2011-01-01

    OBJETIVO: Verificar a associação entre a presença de eosinofilia e a soropositividade para anticorpos IgG anti-Toxocara spp. em crianças atendidas pelo Sistema Único de Saúde no Noroeste do Paraná, Brasil. MÉTODOS: Estudo retrospectivo com crianças de sete meses a 12 anos, atendidas pelo Sistema Único de Saúde do Noroeste do Paraná, com teste ELISA para a pesquisa de anticorpos IgG anti-Toxocara spp. e contagem de eosinófilos (eosinofilia >600 células/mm³). RESULTADOS: Entre as 1.199 crianças...

  11. Whole-Blood Counting Immunoassay as a Short-Turnaround Test for Detection of Hepatitis B Surface Antigen, Anti-Hepatitis C Virus Antibodies, and Anti-Treponema pallidum Antibodies

    OpenAIRE

    Kudo, Toyoichiro; Kido, Aiko; Nishiyama, Yukiko; Koganeya, Hiroshi; Okuda, Takako; Nabeshima, Motoshige; Iinuma, Yoshitsugu; Ichiyama, Satoshi

    2004-01-01

    Whole-blood samples were used for a counting immunoassay (CIA) with the aim of developing a short- turnaround test. After optimization of the CIA, hepatitis B surface antigen (HBsAg), anti-hepatitis C virus antibodies (anti-HCV), and anti-Treponema pallidum antibodies (anti-TP) were detected as efficiently as by an enzyme immunoassay (EIA) with serum samples. The correlations between whole-blood CIA and serum EIA were 99.8, 97.1, and 99.4% for HBsAg, anti-HCV, and anti-TP, respectively. Whole...

  12. "Anti-HBc alone" in human immunodeficiency virus-positive and immuno-suppressed lymphoma patients

    Institute of Scientific and Technical Information of China (English)

    Yu Xuan Koo; Daniel SW Tan; Iain BH Tan; Richard Quek; Miriam Tao; Soon Thye Lim

    2009-01-01

    Hepatitis B virus (HBV) infection is endemic in various parts of the world. A proportion of patients have resolved prior exposure to HBV, as evidenced by the clearance of circulating hepatitis B surface antigen and the appearance of antibody to hepatitis B core antigen (anti-HBc), which could produce protective antibody to hepatitis B surface antigen (anti-HBs). With time, anti-HBs in some patients may become negative. Such patients are described as having occult HBV infection or "anti-HBc alone". In the context of immunodeficient patients, such as HIV patients or lymphoma patients undergoing immunosuppressive immunotherapy, the lack of protective anti-HBs may increase the risk of hepatitis B reactivation. Serum HBV DNA testing may be necessary in "anti-HBc alone" patients, to detect patients at a high risk of developing HBV infection allowing appropriate prophylactic management.

  13. HBsAg检测结果弱反应的分析与处置

    Institute of Scientific and Technical Information of China (English)

    彭静; 管青; 王斌; 王炼; 孙自镛

    2008-01-01

    目的 通过对某国产定性试剂检测HBsAg弱反应结果的分析,探讨HBsAg检测的影响因素及"灰区"的设定.方法 用某国产ELISA试剂对140 709份血清样本进行HBVM定性检测,对HBsAg弱反应结果样本再用Abbott公司试剂定量检测HBVM.对定性和定量结果不相符的样本用另一种定性试剂验证.对部分弱反应定性结果样本进行HBV DNA检测,同时采用定性和定量两种试剂测定一系列HBsAg定值参比血清,比较其灵敏度.结果 140 709份样本中HBsAg可疑者174份(0.124%),弱阳性者126份(0.089%).对其中49份可疑样本和41份弱阳性样本进行定量检测,结果阳性者分别为39份和32份,符合率分别为79.59%和78.05%.17份HBsAg定性弱反应性而定量阴性的样本经另一种定性试剂验证,结果2份为弱反应性,其中1份样本为HBV DNA阳性.对85份弱反应性样本进行HBV DNA检测,其中阳性3份.某国产ELISA试剂检测HBsAg灵敏度为0.5ng/ml,Abbott试剂为0.05 mg/ml.结论 我国的HBV感染人群中,有一部分血清HBsAg呈低水平状态存在,并且部分感染者存在病毒复制.国产HBsAg ELISA定性试剂灵敏度较Abbott定量试剂低,因此必须重视弱反应性结果,尤其是位于阳性判断值以下者,灰区设置非常必要.

  14. Persistance of HBsAg and serum activities of liver enzymes among chronic carriers of hepatitis B

    OpenAIRE

    Mansoureh Momen-Heravi; Hossein Akbari

    2011-01-01

    Background: Hepatitis B virus (HBV) infection and its squeals are major global health problems. This study was conducted to evaluate HBsAg persistence, risk factors of HBV infection and changes of liver function tests among chronic HBV carriers in Kashan. Materials and Method: This descriptive study was performed in HBsAg positive blood donors who detected after blood donation since 10 years ago. They were requested to retest HBsAg. A questionnaire consisting demographic and history of risk ...

  15. Discordant diagnostic results due to a hepatitis B virus T123A HBsAg mutant.

    Science.gov (United States)

    Osiowy, Carla; Kowalec, Kaarina; Giles, Elizabeth

    2016-07-01

    HBsAg immunoassay results are occasionally discordant among primary and confirmatory assays or with respect to other markers of HBV infection. Such discordance has been observed repeatedly in Canada with samples having a mutation at HBsAg codon 123 (sT123A). Detection of recombinant expressed HBsAg protein having either sT123 or sA123 was evaluated with one manual and six automated HBsAg immunoassays. The recombinant mutant HBsAg was non-reactive by Abbott AxSYM, while the Abbott ARCHITECT Quantitative and Qualitative II, ADVIA Centaur, and VITROS ECi detection signal was reduced compared with the wild-type protein, approaching the assay cut-off for certain assays, dependent upon the level of protein. The Roche Elecsys and manual immunoassays detected both wild-type and mutant proteins comparatively. The sT123A mutation leads to loss of detection by immunoassays commonly used in Canadian diagnostic laboratories, which may produce misleading results and diagnoses. PMID:27133305

  16. The optimized of ELISA examines HBsAg the experimental condition%ELISA法检测HBsAg实验条件的优化

    Institute of Scientific and Technical Information of China (English)

    郭翀; 张真铭

    2011-01-01

    To analysis temperature,the incubating time,the vibration examine HBsAg to the influence of the ELISA method,further in expectation of optimizes the experimental conditions. Methods The HBsAg blood serum specimen were used,which density approximately was 1 ng/mL,in four kinds of different experimental conditions (37 ℃ vibration, 37 ℃ no vibration,25 ℃ room temperatures vibrations,25 ℃ room temperatures no vibration) and three different reaction time (10 minutes, 20 minutes,30 minutes)determine HBSAg,and register the numerical value of the absorbency. Results except 37 ℃ no vibration and 25 ℃ room temperatures no vibration exzist no apparent differences(P>0.05), they exzist the apparent differences(P<0.05).Conclusion 37 ℃ and vibrating 20 minutes is the best optimized condition of this experiment.%目的 探讨温度、孵育时间、振荡对ELISA法检测HBsAg的影响,以期进一步优化检测条件.方法 采用HBsAg含量约为1 ng/mL的血清标本,在4种不同条件(37 ℃振荡、37 ℃不振荡、25 ℃室温震荡、25 ℃室温不振荡)下的3个不同反应时间(10、20、30 min)测定HBsAg,记录吸光度值.结果除10 min 37 ℃不振荡和25 ℃不振荡差异无统计学意义(P>0.05)外,其余各组差异都有统计学意义(P<0.05).结论 37 ℃振荡20 min为ELISA检测HBsAg的较佳条件.

  17. Uso de anticuerpos contra proteínas extracelulares de bacterias de la microbiota intestinal como biomarcadores de Enfermedad Inflamatoria Intestinal

    OpenAIRE

    Hevia González, Arancha

    2012-01-01

    En este trabajo se pretende utilizar los niveles de anticuerpos dirigidos contra bacterias ingeridas con los alimentos, representantes de la microbiota intestinal, como posibles biomarcadores para uso en la Enfermedad Inflamatoria Intestinal con el fin de facilitar el desarrollo de mejores métodos de diagnóstico precoz.

  18. Anti-HBV prevalence in firstyear students, that will practise professions of high risk of infection with the HBV

    OpenAIRE

    Ioannis Ioannidis; Melpomeni Ioannidou; Christodoulos Likartsis; Assimoula Siochou; Penelope Siourda

    2007-01-01

    Aim: We have studied the prevalence of Hepatitis B Virus on the first‐year students of the Paramedical Schools (Medical Lab Department, Nursing Department, Baby Nursing Department and Obstetrics Department of the Health and Foresight Professions School of Technological Educational School of Thessaloniki). Materials and Methods: The sample consists of 502 students of the Paramedical Schools. They were taken serum sample for quantitative determination of HbsAg, Anti-HBs, Anti-HBc. The samples w...

  19. Evaluación de anticuerpos contra enfermedades prevenibles por vacunas en el binomio madre–recién nacido en hospitales de Ciudad de La Habana

    Directory of Open Access Journals (Sweden)

    Rolando Felipe Ochoa

    2007-08-01

    Full Text Available El sistema inmune es inmaduro en los recién nacidos, lo que los hace susceptibles a las enfermedades infecciosas, sin embargo, pueden ser parcialmente protegidos por los anticuerpos adquiridos a través de la placenta. Por ello, nos propusimos evaluar la transferencia transplacentaria de anticuerpos contra algunas enfermedades prevenibles por vacunas. Se realizó un ensayo prospectivo, aleatorio, en el binomio madre–recién nacido. Para ello se analizó un total de 96 pares de muestras de suero, tomadas en el salón de preparto y al nacimiento en hospitales de Ciudad de La Habana, durante el año 2004. Se emplearon técnicas inmunoenzimáticas para determinar los niveles de antitoxina tetánica (ATT, antitoxina diftérica (ATD y anticuerpos contra proteínas de membrana externa del meningococo B (PME, contra el polisacárido capsular del meningococo C (PSC y contra el polisacárido Vi de Salmonella Typhi. Se detectó un nivel apropiado de ATT, tanto en las madres como en los recién nacidos, predominando los valores correspondientes a la protección de larga duración (>1 UI/mL, con 88,54% y 92,71%, respectivamente. Se observó niveles de ATD inferiores a 0,1 UI/mL, límite para una protección confiable, en el 54,17% y 45,83% de las madres y los recién nacidos. El 90,63% de las madres y el 96,88% de los recién nacidos presentaron concentraciones de anticuerpos contra PME superiores a 2 µg/mL, así como el 38,54% y el 41,67% para anticuerpos contra PSC. La seroprotección contra la Salmonella Typhi (>1 µg/mL fue de 5,21% y 8,33%, respectivamente. La mayoría de los recién nacidos presentaron títulos superiores de anticuerpos al de sus madres, lo cual demuestra que la transferencia transplacentaria de anticuerpos es un mecanismo eficiente.

  20. Prevalencia de anticuerpos contra Leptospira en personas asintomáticos y en perros de Chancay, Lima 2001

    Directory of Open Access Journals (Sweden)

    Manuel Céspedes

    2007-10-01

    Full Text Available Objetivos. Determinar la prevalencia de anticuerpos contra Leptospiras en personas asintomáticas dedicadas a la agricultura, pesca y comercio y en perros domésticos de localidades de Chancay (Huaral, Lima. Material y métodos. Estudio transversal analítico, se tomó muestras de suero de 268 pobladores de tres localidades, en quienes se evaluó la presencia de anticuerpos totales contra Leptospiras por el método de ELISA IgG y la prueba de microaglutinación (MAT. Se buscaron los factores asociados con la positividad a Leptospiras calculando el OR con su intervalo de confianza al 95%. De la misma manera, se tomó muestras de suero de 241 perros a los que se realizó la prueba de MAT. Resultados. La prevalencia de anticuerpos contra Leptospira en población asintomática fue de 10,1% (IC95%: 6,3-13,9, la cual estuvo asociada con el abastecimiento de agua para consumo en quebrada o pozo (OR: 3,48, IC95%: 1,39-8,74, con el antecedente de nadar en el río o acequia (OR: 4,75, IC95%: 1,51-14,92 y con tener una edad entre 21-40 años (OR: 2,47, IC95%: 1,10-5,52. Los serovares más frecuentes fueron Icterohaemorrhagiae y Canicola según MAT. En canes, 27,8% (67/241 tuvieron serología positiva a leptospiras. Conclusiones. Existe una mediana prevalencia de serología positiva para Leptospiras en la población general asintomática y condiciones favorables para la presencia de Leptospiras en las localidades estudiadas. En estas zonas se recomienda realizar actividades educativas preventivas frecuentes, tomando en cuenta los resultados de este estudio y el personal de salud debe sospechar de la leptospirosis como una causa de enfermedad febril.

  1. Comparison of three different recombinant hepatitis B vaccines: GeneVac-B, Engerix B and Shanvac B in high risk infants born to HBsAg positive mothers in India

    Institute of Scientific and Technical Information of China (English)

    Vijayakumar Velu; Subhadra Nandakumar; Saravanan Shanmugam; Suresh Sakharam Jadhav; Prasad Suryakant Kulkarni; Sadras Panchatcharam Thyagarajan

    2007-01-01

    AIM: To evaluate a low cost Indian recombinant hepatitis B vaccine GeneVac-B for its immunogenicity and safety in comparison to Engerix B and Shanvac B vaccine in high risk newborn infants born to (hepatitis B surface antigen) HBsAg positive mothers.METHODS: A total of 158 infants were enrolled in the study. Fifty eight infants were enrolled in the GeneVac-B group while 50 each were included for Engerix B and Shanvac B groups. A three-dose regimen of vaccination; at birth (within 24 h of birth), 1st mo and 6 mo. were adopted with 10 ng dosage administered uniformly in all the three groups. Clinical and immunological parameters were assessed for safety and immunogenicity of the vaccines, in all the enrolled infants.RESULTS: Successful follow up until seven months of age was achieved in 83% (48/58) for GeneVac-B, 76% (38/50) and 64% (32/50) for Engerix B and Shanvac B groups respectively. 100% seroconversion and seroprotection was achieved in all the three groups of infants. The geometric mean titers of anti-HBs one month after the completion of three dose of vaccination were 90.5, 80.9 and 72.5 mlU/mL in GeneVac-B, Engerix B and Shanvac B vaccine group respectively. Furthermore the level of anti-HBs increases with age of babies who were born to HBsAg positive mothers. The GMT values of anti-HBs were 226.7, 193.9 and 173.6 mlU/mL respectively in GeneVac-B, Engerix B and Shanvac B groups one year after the completion of the three doses of vaccine. No systemic reactions were reported in infants during the entire vaccination process of GeneVac-B and the other two vaccines. Clinical safety parameters remained within the normal limits throughout the study period.CONCLUSION: The study concludes that there is no significant difference between the three recombinant hepatitis B vaccines. Administration of these vaccines within 24 h of birth to babies, born to HBsAg positive mothers will reduce the incidence of HBV infection.

  2. A Study on HBsAg Carrier Rate of Children under Age Five in Danzhou City

    Institute of Scientific and Technical Information of China (English)

    Bo Hu; Dui-Liu Li; Yuan-Gui Feng

    2015-01-01

    Objective:To learn the state of HBsAg carrier after implementing vaccine hepatitis B immunization program for children in Danzhou, and providing a scientific basis for the development of the hepatitis B vaccination strategies.Methods:A combination of stratified sampling and mechanical sampling method was carried out, the authors surveyed vaccine immunization history with hepatitis B in these children who were born from January 1, 2008 to December 31, 2012, then collected blood 3 mL and detected HBsAg carrier status by ELISA from these surveyed children.Results:250 children were included in the survey, the 24 h of birth Hepatitis B vaccine coverage rate in urban, rural newborns was 97.6% and 93.6%, respectively, hepatitis B vaccine three-pin vaccination rate for the whole vaccination course were 100%, only two children from rural area HBsAg carrier was positive, and their positive carrier rate was 0.8%, below 1%. Two HBsAg-positive carrier children from rural area and their families were under epidemiological survey, with discovery that their mothers were carriers of HBsAg, considered as mother to child transmission.Conclusions:Strengthen propaganda promotion to expand knowledge of Hepatitis B and to improve neonatal hepatitis B vaccine 24 h of the first dose timely rate, and standardize hepatitis B vaccine three-pin qualified vaccination rate for the whole vaccination course in children. Take effective measures to improve PMTCT rate, it can reduce child carrier rate of hepatitis B surface antigen, and take effective measures to improve PMTCT rates, it will reduce children carrier rate of HBsAg.

  3. Cinética y capacidad neutralizante de los anticuerpos homólogos y heterólogos contra serotipos de virus dengue en relación con el intervalo entre las infecciones, la secuencia de infección, duración de la viremia y la amplificación dependiente de anticuerpos en infecciones primarias y secundarias

    OpenAIRE

    Alvarez Vera, Mayling

    2008-01-01

    Para sustentar el papel de la infección secundaria en la etiopatogenia del Dengue y Dengue Hemorrágico en epidemias por Dengue 2 y 3 y la influencia de la secuencia de infección e intervalo de tiempo sobre la sensibilización , evaluamos la presencia de anticuerpos específicos contra serotipos, la cinética de anticuerpos neutralizantes en sueros inmunes a Dengue 1 colectados 4 y 20 años después de la primoinfección, la amplificación dependiente de anticuerpos después de 20 años, la capacidad n...

  4. Passive-active immunisation of neonates of HBsAg positive carrier mothers: preliminary observations.

    OpenAIRE

    Mazel, J A; Schalm, S W; de Gast, B C; Nuijten, A S; Heijtink, R A; Botman, M J; Bänffer, J R; Gerards, L.J.; Zwijnenberg, J; Mettau, J

    1984-01-01

    Screening of pregnant women for hepatitis B surface antigen (HBsAg) in three areas of Holland led to the identification of HBsAg carriers, 20 of whom were subsequently delivered. Within two hours after birth all infants received hepatitis B immune globulin (0.5 ml/kg body weight) and, after randomisation, hepatitis B vaccine (10 micrograms) was given either at 0, 1, and 2 months of age or at 3, 4, and 5 months of age, the latter concomitantly with DPTP vaccination. Eighteen infants complying ...

  5. 透析患者血清HBsAg假阳性伴HIV可疑一例分析%Analysis of a Case of Serum False-Positive HBsAg and Suspicious HIV Antibody in Dialysis Patient

    Institute of Scientific and Technical Information of China (English)

    吴卫; 许少侠; 黄媛; 郭野; 司永珍; 杨卓; 谢田; 王巧凤; 崔巍

    2012-01-01

    HBsAg和HIV抗体的检测分别在HBV感染和HIV感染的诊疗中具有重要意义.现阶段HBsAg和HIV抗体主要通过血清免疫学方法检测,其中ELISA法检测HBsAg或HIV抗体的假阳性病例时有报道,而全自动化学发光免疫分析仪的假阳性病例较为少见.该文即报道了一例用全自动化学发光免疫分析仪检测透析患者血清HBsAg假阳性伴HIV抗体可疑的病例,并对可能的原因进行了深入探讨.%HBsAg and HIV antibody play important role in the diagnosis and treatment of HBV and HIV infection respectively. At present, HBsAg and HIV antibody are mainly detected by serum immunological techniques. Cases of false-positive HBsAg or HIV antibody detected by ELISA were reported occasionally while false-positive cases by automatic chemilumi-nescence immune analyzers were reported rarely. A case of serum false-positive HBsAg and suspicious HIV antibody detected by automatic chemiluminescence immune analyzers in dialysis patient is reported in t paper and analyzed for the possible reasons.

  6. Construction and immunological evaluation of truncated hepatitis B core particles carrying HBsAg amino acids 119–152 in the major immunodominant region (MIR)

    Energy Technology Data Exchange (ETDEWEB)

    Su, Qiudong; Yi, Yao [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China); Guo, Minzhuo [Beijing Entry-Exit Inspection and Quarantine Beureau, Tianshuiyuan Lane 6, Chaoyang District, Beijing 100026 (China); Qiu, Feng; Jia, Zhiyuan; Lu, Xuexin; Meng, Qingling [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China); Bi, Shengli, E-mail: shengli_bi@163.com [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China)

    2013-09-13

    Highlights: •The conformational HBV neutralization antigen domain was successfully displayed on the surface of truncated HBc particles. •Appropriate dialysis procedures to support the renaturing environment for the protein refolding. •Efficient purification procedures to obtain high purity and icosahedral particles of mosaic HBV antigen. •Strong immune responses not only including neutralization antibody response but also Th1 cell response were induced in mice. -- Abstract: Hepatitis B capsid protein expressed in Escherichia coli can reassemble into icosahedral particles, which could strongly enhance the immunogenicity of foreign epitopes, especially those inserted into its major immunodominant region. Herein, we inserted the entire ‘α’ antigenic determinant amino acids (aa) 119–152 of HBsAg into the truncated HBc (aa 1–144), between Asp{sup 78} and Pro{sup 79}. Prokaryotic expression showed that the mosaic HBc was mainly in the form of inclusion bodies. After denaturation with urea, it was dialyzed progressively for protein renaturation. We observed that before and after renaturation, mosaic HBc was antigenic as determined by HBsAg ELISA and a lot of viruslike particles were observed after renaturation. Thus, we further purified the mosaic viruslike particles by (NH{sub 4}){sub 2}SO{sub 4} precipitation, DEAE chromatography, and Sepharose 4FF chromatography. Negative staining electron microscopy demonstrated the morphology of the viruslike particles. Immunization of Balb/c mice with mosaic particles induced the production of anti-HBs antibody and Th1 cell immune response supported by ELISPOT and CD4/CD8 proportions assay. In conclusion, we constructed mosaic hepatitis core particles displaying the entire ‘α’ antigenic determinant on the surface and laid a foundation for researching therapeutic hepatits B vaccines.

  7. Quantitation of HBsAg predicts response to entecavir therapy in HBV genotype C patients

    Institute of Scientific and Technical Information of China (English)

    Etsuro Orito; Kei Fujiwara; Hiroshi Kanie; Tesshin Ban; Tomonori Yamada; Katsumi Hayashi

    2012-01-01

    AIM:To analysis the factors that predict the response to entecavir therapy in chronic hepatitis patients with hepatitis B virus (HBV) genotype C.METHODS:Fifty patients [hepatitis B e antigen (HBeAg)-negative:HBeAg-positive =26:24] with HBV genotype C,who received na(i)ve entecavir therapy for > 2 years,were analyzed.Patients who showed HBV DNA levels ≥3.0 log viral copies/mL after 2 years of entecavir therapy were designated as slow-responders,while those that showed < 3.0 log copies/mL were termed rapidresponders.Quantitative hepatitis B surface antigen (HBsAg) levels (qHBsAg) were determined by the Architect HBsAg QT immunoassay.Hepatitis B core-related antigen was detected by enzyme immunoassay.Pre-C and Core promoter mutations were determined using by polymerase chain reaction (PCR).Drug-resistance mutations were detected by the PCR-Invader method.RESULTS:At year 2,HBV DNA levels in all patients in the HBeAg-negative group were < 3.0 log copies/mL.In contrast,in the HBeAg-positive group,41.7% were slow-responders,while 58.3% were rapid-responders.No entecavir-resistant mutants were detected in the slow-responders.When the pretreatment factors were compared between the slow-and rapid-responders;the median qHBsAg in the slow-responders was 4.57log IU/mL,compared with 3.63 log IU/mL in the rapidresponders (P < 0.01).When the pretreatment factors predictive of HBV DNA-negative status at year 2 in all 50 patients were analyzed,HBeAg-negative status,low HBV DNA levels,and low qHBsAg levels were significant (P < 0.01).Multivariate analysis revealed that the low qHBsAg level was the most significant predictive factor (P =0.03).CONCLUSION:Quantitation of HBsAg could be a useful indicator to predict response to entecavir therapy.

  8. The Association of Maternal HBsAg Carrier Status and Perinatal Outcome

    Directory of Open Access Journals (Sweden)

    Soraya Saleh-Gargari

    2009-09-01

    Full Text Available Background and Aims: Hepatitis B virus infection is still a major public health concern all over the world, and much research must be carried out on the various aspects of this issue. Since infection with hepatitis B virus in pregnant mothers is a threat for both mother and her fetus, this study was performed to determine the relationship between maternal HBsAg carrier status and perinatal outcome..Methods: A retrospective case-control study was performed on 450 carriers of hepatitis B surface antigen (HBsAg pregnant women and compared to 450 controls. Both groups were matched for age, parity, and body mass index (BMI..Results: When compared to the control group, patients with HBsAg displayed significantly higher incidence of gestational diabetes mellitus (GDM (7.7% vs. 2% P=0.001, increased hospitalization period after delivery (22.9% vs. 3.33%, P<0.0001, preterm labor at less than 37 weeks (10.9% vs. 2.67%, P<0.0001, pregnancy induced hypertension (13% vs. 2.89%, P<0.0001, and preterm premature rupture of membranes (3.55% vs. 1.1%, P=0.03. Also, the incidence of macrosomy (6.67% vs. 2.22%, P=0.02, intrauterine fetal death (5.56% vs. 0.44%, P=0.001, still birth (2.89% vs. 0.44%, P=0.005, and NICU admission (25.78% vs. 2.22%, P<0.0001 in the carrier mothers were higher..Conclusions: HBsAg carriers have increased risk of hospitalization period after delivery, preterm labor, gestational hypertension, preterm premature rupture of membranes. In addition higher incidence of macrosomy, intrauterine fetal death, still birth, and NICU admission were observed. Therefore our results showed HBsAg carrier mothers have increased maternal and neonatal complications.

  9. Frekuensi HBsAg Positif pada Uji Saring Darah di Palang Merah Indonesia Cabang Padang Tahun 2012

    Directory of Open Access Journals (Sweden)

    Nadia Ventiani

    2015-09-01

    Full Text Available Abstrak Infeksi virus hepatitis B dapat berkembang menjadi hepatitis kronis, sirosis hati, kanker hati dan bahkan kematian. Salah satu cara penularannya adalah melalui transfusi darah. Tujuan penelitian ini adalah untuk mengetahuifrekuensi HBsAg positif pada uji saring darah donor.  Telah dilakukan penelitian deskriptif dengan menggunakan datasekunder di PMI Padang dengan 26.975 darah donor yang diteliti. Donor laki-laki lebih banyak dari donor perempuandengan perbandingan 11,69:1, dan pendonor sukarela lebih banyak dari pendonor pengganti, dengan perbandingan2,95:1. Pendonor terbanyak terdapat pada kelompok usia dibawah 30 tahun, yaitu sebanyak 38,09%. Hasil penelitianmenunjukan persentase darah donor dengan HBsAg positif sebesar 3,61%. Pendonor laki-laki dengan HBsAg positifsebesar 93,22%, perempuan 6,78% dan pendonor sukarela sebesar 65,09%, pendonor pengganti sebesar 34,91%.Hasil HBsAg positif terbanyak terdapat pada kelompok usia dibawah 30 tahun sebesar 39,01%. Sebagian besar darahdonor yang mengandung HBsAg positif terdapat pada kelompok umur di bawah 30 tahun. Frekuensi HBsAg positif lebih banyak pada donor laki-laki dibanding donor perempuan, dan donor sukarela dengan HBsAg positif lebih banyak dibanding donor pengganti.Kata kunci: HBsAg, donor darah, transfusi darahAbstract Hepatitis B virus infection could progress into chronic hepatitis, liver cirrhosis, liver cancer and even death. One mode of transmission is via blood transfusion. The objective of this study was to determine the frequency of positive HBsAg in the screening test of the blood donors. A descriptive studies has been conducted by using secondary datasin  PMI Padang. There were 26975 blood donors studied that  men donors were higher than female donors, with the number ratio of male and female were 11.69:1, and the number of voluntary donors were higher compared to the replacement donors, with ratio 2.95:1. Most of the donors were in the age group below 30 years, which

  10. Obtención de un anticuerpo contra la proteína p65 del virus de la tristeza de los cítricos y resultados preliminares de la expresión in vivo Obtaining a citric tristeza virus p65 protein antibody and preliminary results of p65 in vivo expression

    Directory of Open Access Journals (Sweden)

    Acosta Orlando

    2003-12-01

    Full Text Available El virus de la tristeza de los cítricos (CTV pertenece a la familia Closteroviridae que incluye a los únicos virus vegetales que poseen en su genoma genes homólogos a las proteínas de choque térmico celulares HSP70. Recien­temente se ha demostrado que las proteínas virales HSP70h, como la p65 de CTV, realizan funciones para el ensam­blaje del virión, como proteína de unión a microtúbulos y de movimiento célula a célula. De allí la importancia de desarrollar trabajos relacionados con esta proteína en el CTV, considerado el patógeno más deletéreo para los cítricos. En el presente estudio, fueron utilizados cuatro péptidos sintéticos correspondientes a la región carboxi-terminal de la proteína de choque térmico p65 del virus de la tristeza de los cítricos (CTV, para inmunizar conejos con el objetivo de obtener anticuerpos policlonales. Todos los péptidos utilizados fueron inmunogénicos. Ninguno de los anticuerpos obtenidos reaccionó contra el extracto de plantas no infectadas pero sí detectaron las proteínas p65 presentes en los extractos proteicos de cítricos infectados con el CTV. Se demostró un comportamiento inmunológico del anticuerpo anti p65 que parece ser independiente de la severidad sintomática del aislado de CTV utilizado, sobre la base de la exposición del anticuerpo a dos aislados colombianos con características serológicas diferentes. Se demostró que el ORF que codifica en el CTV para el homólogo de la HSP70 se expresa in vivo aunque el anticuerpo anti p65 solamente detectó extractos proteicos concentrados de plantas infectadas, lo que apoya lo informado en otros estudios sobre la baja concentración de esta proteína en plantas infectadas con el CTV. Es la primera vez que se obtiene en Colombia un anticuerpo policlonal anti CTV y, particularmente, contra p65, proteína que interviene en el ensamblaje y movimiento viral. La adaptación de una técnica de obtención de anticuerpos anti p65 a

  11. Anticuerpos frente a virus West nile y otros virus transmitidos por artropodos en la poblacion del Delta del Ebro

    Directory of Open Access Journals (Sweden)

    Lozano Alvaro

    1998-01-01

    Full Text Available FUNDAMENTOS: El virus West Nile (VWN es un Flavivirus que se transmite al hombre a través de distintas especies de mosquitos y produce brotes y casos esporádicos de enfermedad en distintas regiones del Viejo Mundo, incluída la Cuenca Mediterránea. Las zonas húmedas europeas que acogen aves migratorias procedentes de África constituyen áreas de alto riesgo para esta infección, así como para otras infecciones víricas transmitidas por artrópodos. MÉTODOS: Con objeto de investigar la prevalencia de la infección por el VWN y otros virus de transmisión similar en la población humana del Delta del Ebro, se estudiaron 1037 muestras de suero, obtenidas en 10 localidades de la zona, para presencia de anticuerpos frente a VWN y otros 12 virus transmitidos por artrópodos (3 Alfavirus, 8 Flaviviridae y 1 Bunyaviridae mediante titulación por inhibición de la hemaglutinación (IHA. En algunos casos se estudió la presencia de IgM específica por IHA tras fraccionar el suero por centrifugación en gradientes de sacarosa. RESULTADOS: En total, se encontró reactividad significativa frente a alguno de los virus probados en 130 casos (12.5%; 4.1% frente a Alfavirus, 8.0% frente a Flaviviridae y 0.4% frente a Bunyaviridae. El análisis de los títulos de anticuerpos reveló porcentajes significativos de muestras con títulos elevados frente a antígenos de VWN y otros. La distribución de la seroprevalencia fue muy desigual, concentrándose fundamentalmente en 3 localidades del interior del Delta (Ampolla, San Jaime y Montells, donde la prevalencia de anticuerpos frente a Flaviviridae llegó a alcanzar el 30% y se observaron niveles residuales de IgM frente a VWN en algunos sueros. CONCLUSIONES: Estos resultados y los obtenidos previamente en otras regiones de la Península Ibérica sugieren que el VWN circula en la población humana de las zonas de riesgo y produce brotes epidémicos periódicos. Habida cuenta del alto porcentaje de

  12. Prevalencia de hepatitis viral A y B y factores de riesgo asociados a su infección en la población escolar de un distrito de Huánuco - Perú

    Directory of Open Access Journals (Sweden)

    C Heriberto Hidalgo

    2002-01-01

    Full Text Available Objetivo: Determinar la prevalencia de marcadores serológicos para la infección por el virus de la hepatitis A y B en la población escolar del distrito de Huánuco e identificar los factores asociados a dichas infecciones. Materiales y métodos: en este estudio transversal analítico se seleccionó una muestra aleatoria y estratificada de 270 del total de escolares registrados en los diferentes centros educativos del distrito de Huánuco, Departamento de Huánuco-Perú, de abril a diciembre del 2000, en quienes se evaluó la presencia de HBsAg y anticuerpos anti-HAV, anticuerpos totales, anticuerpos IgM anti-HBcAg, anti-HDV y antígeno "e" en sangre (Estos últimos tres sólo a los HBsAg reactivos. La presencia de factores de riesgo para la infección por estos dos virus fue evaluada por una encuesta epidemiológica. Resultados: 257 (95,2% escolares tuvieron Anticuerpos anti-HAV, 8 (3,0% resultaron ser portadores de HBsAg, 62 (23,0% tuvieron anticuerpos anti- HBcAg y ninguno de los 8 portadores de HBsAg tuvieron anticuerpos anti-HDV, anticuerpos IgM anti-HBcAg, ni antígeno "e" (HBeAg. La edad mayor a 11 años estuvo asociada a la presencia de anti-HAV (OR=14,3, p<0,001. El tener vivienda de adobe estuvo asociado a la reactividad al HBsAg (OR=5,1, p=0,045 y el tener relaciones sexuales estuvo asociado a la presencia de anticuerpos anti-HBcAg (OR=6,49, p=0,003. Conclusiones: el distrito de Huánuco tiene una alta endemicidad para HAV y endemicidad intermedia para el HBV. La edad mayor de 11 años estuvo asociada a una mayor infección por HAV y el tener vivienda precaria y relaciones sexuales a una mayor infección por HBV.

  13. HYDRODYNAMICALLY BALANCED SYSTEMS (HBS): INNOVATIVE APPROACH OF GASTRO RETENTION: A REVIEW

    OpenAIRE

    Dubey Vivek; Arora Vandana; Singh Amit kumar

    2012-01-01

    The objective of writing this review on hydrodynamically balanced systems (HBS) was collection of the recent literature. The design of hydrodynamically balanced drug delivery system is base on prolong GI residence time of drug in an area of the GI tract to maximize drug reaching its absorption. Gastrointestinal transit time of orally administered dosage forms are controlled by using gastro retentive drug delivery systems (GRDDS). Need for GRDDS a controlled drug delivery system with prolonged...

  14. Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg

    OpenAIRE

    Jackson Marcondes; Ekkehard Hansen

    2008-01-01

    The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons wit...

  15. Induced HBs antigenemia in healthy adults after immunization with two different hepatitis B recombinant vaccines

    OpenAIRE

    Masoud ZIAEE; Saádatjoo, Alireza; Mohamadpour, Malihe; Namaei, Mohammad Hasan

    2010-01-01

    Background and Aims Currently, vaccination is the most effective protective tool against hepatitis B virus infection. Some studies have shown that positive results for a hepatitis B virus surface antigen (HBsAg) test may be seen after vaccination. Materials and Methods In this clinical trial study, 62 healthy adult volunteers were randomly assigned to receive either the Engerix-B or the Hepavax-Gene hepatitis B recombinant vaccine. Blood samples were drawn 1, 3, and 5 days after vaccination a...

  16. Prevalence of HBsAg Positive among Afghan Sweepers in Tehran, 2009

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    Seyed Mohammad Javad Hosseini

    2013-07-01

    Full Text Available AbstractBackground and objective: Hepatitis B virus is one of the great public health problems all over the world. Moreover prevalence of Hepatitis B in Iran is near 1.5 million (2.03% of general population. The objective of this study was to determine the prevalence of Hepatitis B among Afghan sweepers in 2, 5 and 9 municipal areas of Tehran in 2009.Materials and methods: This was a cross-sectional study on 250 afghan sweeper in 2, 5 and 9 municipal areas of Tehran. Demographic form including age, duration of working and migration, history of sexual contacts, needle stick injury, IV drug abuse, surgery and blood transfusion was completed for all cases. The blood samples were collected and examined for HBsAg by Eliza test at Iran Blood transfusion organization laboratory.Results: From 250 Afghan sweepers enrolled in this study, HBsAg positive was detected in 10 (4% cases. We found significant relation between the HBsAg positive, needle stick injury, unprotected sexual contact, age and time migration to Iran (P<0.05.Conclusion: Prevalence of Hepatitis B was approximately high among afghan sweeper in Tehran. The results indicated that prevalence of risk factors like needle stick injury and unprotected sexual contact were high among Afghan sweepers. Educations of Hepatitis B, ways of transmission, screening of infectious disease, and other blood-born infection to sweepers are important.

  17. Development of a Novel, Ultra-rapid Biosensor for the Qualitative Detection of Hepatitis B Virus-associated Antigens and Anti-HBV, Based on “Membrane-engineered” Fibroblast Cells with Virus-Specific Antibodies and Antigens

    Directory of Open Access Journals (Sweden)

    Antonios Perdikaris

    2009-03-01

    Full Text Available A novel miniature cell biosensor detection system for the detection of Hepatis B virus (HBV-associated antigens and anti-HBV is described. The biosensor is based on “membrane-engineered” Vero fibroblast cells immobilized in an alginate matrix. The membrane-engineering process involved the electroinsertion of anti-HBV specific antibodies (anti-HBs, anti-HBe or antigens (HBsAg in the membranes of the Vero cells. The attachment of a homologous antigen to the electroinserted antibody (or, respectively, of the antibody to the electroinserted antigen triggered specific changes to the cell membrane potential that were measured by appropriate microelectrodes, according to the principle of the Bioelectric Recognition Assay (BERA. The sensor was used for screening 133 clinical blood serum samples according to a double-blind protocol. Considerably higher sensor responses were observed against HBV-positive samples, compared with responses against negative samples or samples positive for heterologous hepatitis viruses such as Hepatitis C (HCV virus. Detection of anti-HBs antibodies was made possible by using a biosensor based on immobilized Vero cells bearing the respective antigen (HBsAg. The observed response was rapid (45 sec and quite reproducible. Fluorescence microscopy observations showed that attachment of HBV particles to cells membrane-engineered with anti-HBs was associated with a decrease of [Ca2+]cyt. The perspectives for using the novel biosensor as a qualitative, rapid screening, high throughput assay for HBV antigens and anti-HBs in clinical samples is discussed.

  18. Evaluation of cellular responses for a chimeric HBsAg-HCV core DNA vaccine in BALB/c mice

    Directory of Open Access Journals (Sweden)

    Maryam Yazdanian

    2015-01-01

    Conclusion: Fusion of HBsAg to HCVcp in the context of a DNA vaccine modality could augment Th1-oriented cellular and CTL responses toward a protective epitope, comparable to that of HCVcp (subunit HCV vaccine immunization.

  19. HBsAg detection by passive hemagglutination (Hepanosticon--Organon). Advantages and disadvantages in comparison with other methods.

    Science.gov (United States)

    Balş, M G; Hagiescu, L

    1976-01-01

    Investigated comparatively with immunodiffusion, electroimmunodiffusion, complement fixation and Latex agglutination, passive hemagglutination with the Hepanosticon--Organon reagent proved to be an easy, rapid, highly reproducible method for HBsAg detection.

  20. A Intervention Study to Improve HBsAg Testing and Preventive Practices for Hepatitis B in an Obstetrics Hospital

    Directory of Open Access Journals (Sweden)

    Ibrahim Koruk

    2011-06-01

    Full Text Available Objectives: This study was conducted to determine the effectiveness of a multicomponent intervention in Sanliurfa Gynecology and Obstetrics Hospital (SGOH, which is designed to overcome the obstacles encountered in the application of HBsAg testing to all delivering women, the administration of hepatitis B vaccine and Hepatitis B Immunglobulin(HBIG to newborns of HBsAg positive pregnant women. Methods: This is an intervention study, conducted between December 15, 2007 and December 30, 2008. Intervention procedure is composed of four methods; namely, motivation of all parties through academic leadership, implementation of workshops to determine the obstacles, provision of administrative commitment, and training of healthcare personnel. Results: In 2007, a total of 18,709 deliveries were realized in SGOH; HBsAg testing was performed in only 16.5% of the pregnant women and 0.8% of these women were found to be HBsAg positive. It was determined that HBIG was administered to 28.2% of newborns of HBsAg positive mothers and hepatitis B vaccine was given to 71.4% (n=13349 of all newborns. After the intervention, the increase in HBsAg test application status, HBsAg positivity, HIBG administration and hepatitis B vaccination was 6.0, 5.8, 2.0 and 1.2 fold, respectively (P<0.05. Conclusions: In order to reduce the incidence of hepatitis B and maintain patient safety in newborns, HBsAg testing should be included in routine pregnancy screening programs and this approach should be supported by legal arrangements and systematic continuous training activities. [TAF Prev Med Bull 2011; 10(3.000: 287-292

  1. European collaborative evaluation of Enzygnost HBsAg 6.0: performance on hepatitis B virus surface antigen variants

    OpenAIRE

    Avellón, Ana; Echevarría, Jose-Manuel; Weber, Bernard; Weik, Michael; Schobel, Uwe-Peter; Willems, Wulf R.; Gerlich, Wolfram

    2010-01-01

    Abstract Amino acid changes within the major antigenic determinant of the hepatitis B virus (HBV) surface antigen (HBsAg) may eventually modify the antigenic properties of the protein and may have impact on the sensitivity of diagnostic assays. Modifications in the design of an assay can, however, improve significantly its capability to detect these mutants. One-hundred and forty-seven clinical samples containing HBsAg variants, and 54 supernatants of cells expressing recombinant H...

  2. PATTERN OF HBsAg POSITIVITY IN SELECTED GROUPS AT KING KHALID GENERAL HOSPITAL - HAIL REGION, KINGDOM OF SAUDI ARABIA

    OpenAIRE

    Mahaba, Hisham M.; El-Tayeb, Abd El Kader A.; El-Sekibi, Dahl K; El Gofaei, Ali F.; El-Baz, Hisham S.; Ismail, Nanees A.

    1997-01-01

    Background: Hepatitis B virus (HBV) is endemic in the Kingdom of Saudi Arabia. The acquisition of infection occurs early in life. The availability of safe and efficacious vaccines has led to the feasibility of an effective control of HBV infection. This study compares the pattern of HBsAg positivity among selected groups of patients with similar groups in other regions in the Kingdom of Saudi Arabia. Objectives: This study is conducted to determine the prevalence of HBsAg positive subjects am...

  3. Detection of HBsAg, HBcAg, and HBV DNA in ovarian tissues from patients with HBV infection

    Institute of Scientific and Technical Information of China (English)

    Li-Zhang Chen; Xue-Gong Fan; Jian-Ming Gao

    2005-01-01

    AIM: To investigate the presence of HBsAg, HBcAg, and HBV DNA in ovarian tissues from patients with HBV infection.METHODS: HBsAg and HBcAg were examined in ovarian biopsy tissues from 26 patients with HBV infection by immunocytochemistry, and HBV DNA was detected in ovarian tissues by PCR.RESULTS: HBsAg and HBcAg were present with the same positive rate of 34.6% (9/26). The total positive rate was 46.2% (12/26). HBsAg and HBcAg were positive in 6 (23.1%) of the 26 patients. Brown positive particles were diffusely distributed in ovarian cells. The positive rate of HBV DNA was 58.3% (7/12).CONCLUSION: HBsAg, HBcAg, and HBV DNA can be detected in ovarian tissues from patients with HBV infection. The presence of HBsAg and HBcAg in ovarian tissues does not correlate with the HBV markers in serum.

  4. Advanced characterization of MIMAS MOX fuel microstructure to quantify the HBS formation

    Energy Technology Data Exchange (ETDEWEB)

    Bouloré, Antoine, E-mail: antoine.boulore@cea.fr [CEA, DEN, DEC Fuel Research Department, Cadarache, F13108 Saint-Paul-lez-Durance (France); Aufore, Laurence; Federici, Eric [CEA, DEN, DEC Fuel Research Department, Cadarache, F13108 Saint-Paul-lez-Durance (France); Blanpain, Patrick [AREVA NP SAS, 10 rue Juliette Récamier, F-69456 Lyon (France); Blachier, Rémi [EDF, SEPTEN, 12-14 Av. Dutrievoz, F-69628 Villeurbanne (France)

    2015-01-15

    Highlights: • An advanced characterization of MIMAS MOX fuel based only on fresh fuel pellet characterization. • A probabilistic approach to model the High Burnup Structure formation in oxide fuels. • Validation of the method by comparing to experimental data obtained on fuel irradiated in the Halden reactor. - Abstract: Fission gas behaviour in accidental situations is closely related to the location of fission gas before the accident. More precisely, most of the fission gas in intergranular position is released during the accident and HBS zones contribute a lot to this intergranular quantity. So a methodology to characterize the HBS zones a priori from examination of unirradiated pellet has been developed at CEA. Characterization of plutonium distribution in MIMAS MOX fresh fuel pellets can be performed by image analysis on 1 mm{sup 2} X-ray mappings of plutonium acquired using Electron Probe Micro Analysis (EPMA). The specific software developed to describe the fuel using Pu X-ray mapping (ANACONDA) has been improved in order to simulate the fission products (FP) production and recoil during a given irradiation of the fuel, taking into account the evolution of the plutonium due to neutron irradiation. This simulation results from calculations with our fuel performance code ALCYONE combined with image processing. The final result is a mapping of local burn-up, but also the distribution of the relative FP concentration as a function of the local burn-up. A validation of this simulation process has been done by comparing the simulated mapping of neodymium to one measured on the same fuel batch after irradiation. Using previous studies of mechanisms for HBS formation, a probabilistic criterion for HBS formation has been proposed, based on the EPMA measurements of the decrease of the xenon signal as a function of the local burn-up. Combining the simulated FP cartography with this probabilistic HBS formation criterion, it is possible to calculate the surface

  5. Enzyme-linked Immunoassay (ELISA) for Testing and Evaluation of Low Concentration of HBsAg%酶联免疫法(ELISA)对低浓度HBsAg的检测与评价

    Institute of Scientific and Technical Information of China (English)

    韩福红

    2015-01-01

    目的:探讨酶联免疫法(ELISA)在低浓度HBsAg检测中的效果。方法以本院2014年1~12月门诊与住院患者中53例采用科华ELISA法检测HBsAg呈阳性且S/CO≤10的标本为研究对象,对所有标本采用HBsAg确认试剂盒以及配套使用的ELISA法进行检测,比较检测结果。结果53例科华ELISA检测HBsAg阳性患者,经确认试剂盒及其配套ELISA法检测,阳性的45例(84.9%),8例(15.1%)确认试验为阴性,并经科华试剂复测结果亦为阴性。结论对低浓度阳性标本进行HBsAg ELISA法复检,并用中和试验去除假阳性结果,能够有效避免报告错误率,减少手术,输血及器官移植等安全隐患。%ObjectiveTo investigate the enzyme-linked immunoassay (ELISA) in low concentration effect of HBsAg detection.Methods In January 2014~December 2014 at our hospital outpatient and hospitalized patients in 53 patients with progress ELISA method to detect HBsAg positive and S/CO specimens of 10 or less as the research object, for all specimens using HBsAg conifrm kit as wel as supporting the use of ELISA method for testing, comparing test results.Results Kehua ELISA detected 53 cases of HBsAg positive patients, conifrmed kit and supporting by ELISA. Positive results of 45 cases (84.9%), 8 cases (15.1%) of the validation test were negative, and the progress reagent of retest results are negative. Conclusion HBsAg ELISA method for low concentration samples recheck, and neutralization test to remove false positive results, can effectively avoid the report error rate, reduce the surgery, blood transfusion and organ transplantation and other security hidden danger.

  6. Presencia de anticuerpos sericos contra Neospora caninum en equinos en Chile Sera antibodies to Neospora caninum in Chilean horses

    Directory of Open Access Journals (Sweden)

    A.N. Patitucci

    2004-12-01

    Full Text Available Neospora sp. es un parásito protozoario causante de abortos y/o enfermedad del Sistema Nervioso Central (SNC en caninos, bovinos, ciervos, caprinos, ovinos, equinos y otros. En equinos, se lo ha involucrado como causa de aborto, mortalidad neonatal y enfermedades del SNC y viscerales. La especie que infecta a los equinos es distinta a Neospora caninum y se denomina Neospora hughesi. En Chile no existe evidencia de infección en equinos, sin embargo, la enfermedad ha sido informada en bovinos y caninos, por lo que el objetivo de este trabajo fue buscar anticuerpos contra la enfermedad en sueros equinos . Para ello se tomaron muestras de sangre a 145 equinos (87 de la IX Región y 58 de la VIII Región de Chile. Los sueros fueron analizados mediante Test de Aglutinación para Neospora (NAT. Cada suero se diluyó en 1:40 en solución salina tamponada (PBS pH 7.2 conteniendo 2- mercaptoetanol y fueron analizados en 1:40 y 1:80. Los sueros positivos a 1:80 fueron examinados a 1:160 y 1:320. Anticuerpos contra N.caninum (≥ 1:40 se encontraron en 32% (47/145 de los equinos estudiados. Debido a que N.caninum y N.hughesi dan reacción cruzada, ambos o uno de ellos podrían ser los responsables de la seropositividad. Estos resultados indican que equinos provenientes de la VIII y IX regiones de Chile son reaccionantes a la infección de Neospora, por lo que su diagnóstico presuntivo debería ser considerados ante la presencia de cuadros clínicos con sintomatología nerviosa y/o abortos en esta especie.Neospora caninum is a protozoan parasite that affects cattle and dogs, its infections seem to be widely prevalent in Chile. To date, nothing is known of the presence of N. caninum in horse population of the country. In South America, two seroprevalence studies have failed to find antibodies in Argentina and Brazil. The objective of the study was to investigate the presence of N. caninum exposure in horses of Chile. Sera from 145 asymptomatic horses (87

  7. DETECCIÓN DE ANTICUERPOS IgM A LEPTOSPIRA EN HUMANOS EN RIESGO OCUPACIONAL EN VILLAVICENCIO, META

    Directory of Open Access Journals (Sweden)

    Luz Díaz P

    2008-04-01

    Full Text Available Objetivo. Determinar la seroprevalencia a la infección por leptospira en una población humana y los factores de riesgo ocupacional asociados en Villavicencio, Meta. Materiales y métodos. Se aplicó un modelo epidemiológico transversal con selección por conveniencia de grupos e individuos dentro de grupos. Se obtuvieron muestras de sangre por punción cubital de 273 personas correspondientes a 8 grupos de riesgo. Se obtuvo información de factores de riesgo mediante entrevista. Se determinaron anticuerpos IgM mediante ELISA indirecta. Se utilizó una prueba de c2 mediante el programa SPSS 11.0 para Windows. Resultados. La seroprevalencia general fue 19%. Por grupos fue para trabajadores de matadero 7%, veterinarios y auxiliares de clínica de pequeños animales 17%, estudiantes de último año de MVZ 17%, ordeñadores 21%, trabajadores de arrozales 23%, trabajadores de granjas porcícolas 35%, trabajadores de piscícolas 48%. Se encontraron 3 factores asociados, el estrato rural p = 0.0005 ICCR 1.50 a 3.83, tenencia de mascota canina p = 0.046 ICCR 1.03 a 3.26 y contacto con roedores en el trabajo p = 0.000037 ICCR = 1.73 a 4.75. Conclusiones. Se encontró una alta seroprevalencia general de infectados por Leptospira, siendo los grupos de más alta prevalencia los trabajadores piscícolas y de granjas porcícolas. Se reconoce una vez más el carácter ocupacional de la infección posiblemente por falta de medidas de higiene y protección laboral.

  8. Soroprevalência de anticorpos contra vírus herpes simples 1-2 no Brasil Seroprevalencia de anticuerpos contra virus herpes simples 1-2 en Brasil Seroprevalence of herpes simplex 1-2 antibodies in Brazil

    Directory of Open Access Journals (Sweden)

    Sue Ann Costa Clemens

    2010-08-01

    de sangre para detección de anticuerpos para HSV-1 y HSV-2 con prueba tipo-específica ELISA gG1-gG2. Fueron descritas frecuencias y proporciones y comparadas entre grupos utilizando la prueba de Fisher bilateral exacta. Fue realizado análisis de regresión logística para evaluar influencia de las variables edad, sexo, geografía, grupo económico, histórico de DST, seropositividad para anti-HSV-1 o anti-HSV-2 e interacciones de cualquiera de esos factores sobre la seroprevalencia de HSV-1 y/o HSV-2. RESULTADOS: La tasa de seroprevalencia de anticuerpos para HSV-1 ajustada por edad fue de 67,2%, sin diferencia con relación al sexo, siendo mayor en la Región Norte. Las seroprevalencias aumentaron con la edad, y para HSV-2, hube un aumento significativo en la adolescencia y entre adultos jóvenes. Individuos seropositivos para HSV-1 presentaron mayor riesgo de ser positivos para HSV-2 (15,7% cuando se compararon con los negativos para HSV-1 (4,7%. En el análisis multivariado, el histórico de DST aumentó significativamente (OR=3,2 la probabilidad de seropositividad para HSV-2. CONCLUSIONES: Las seroprevalencias para HSV-1 y para HSV-2 varían con la edad y presentan diferencias significativas entre las regiones de Brasil. Historia anterior de DST es importante factor de riesgo para adquisición de infección por HSV-2.OBJECTIVE: To estimate the seroprevalence of HSV-1 and HSV-2 antibodies in Brazil and to analyze factors associated. METHODS: Cross-sectional study including subjects aged 1-40 years from the general population in four different geographical areas in Brazil between 1996 and 1997. All subjects were stratified by age and gender and 1,090 of them were included in the final analysis. Blood samples were tested for HSV-1 and HSV-2 antibodies by type-specific (gG1 and gG2 ELISA. Frequencies and proportions were described and compared among groups using two-sided Fisher's exact test. A logistic regression analysis was performed to assess the influence of

  9. Intra-familial prevalence of hepatitis B virologic markers in HBsAg positive family members in Nahavand, Iran

    Institute of Scientific and Technical Information of China (English)

    Amir Houshang Mohammad Alizadeh; Mitra Ranjbar; Shahin Ansari; Seyed Moayed Alavian; Hamid Mohaghegh Shalmani; Leila Hekmat; Mohammad Reza Zali

    2005-01-01

    AIM: To determine the prevalence of hepatitis B in Nahavand and evaluate the HBsAg positive prevalence in families with a member who was confirmed to have HBV infection.METHODS: This study was performed in two phases. In the first phase, 1 824 subjects in Nahavand city were selected. The interviewers visited the houses of chosen families to fill the questionnaire and take the blood samples.All subjects signed an informed consent before interviews and blood sampling. The samples were evaluated for HBV virologic markers. In the second phase, 115 HBsAg-positive cases were enrolled and evaluated for HBV virologic markers.RESULTS: The prevalence of positive HBsAg in Nahavand was 2.3%. The most frequent relatives of index cases were sons and daughters (32.2% and 23.5% respectively).Twelve (11%) of all family members were HBsAg positive.Fifty (56.2%) were isolated HBsAb positive and only one person (2.5%) was isolated HBcAb positive. The higher rates of HBsAg marker were detected in the brothers (1-25%) and fathers (1-12.5%). The infection rate in husbands and wives of index cases was 10%. Only two (16.7%) of all HBsAg-positive participants reported previous HBV vaccination.CONCLUSION: The prevalence of intra-familial HBV infection is lower in Nahavand of Iran compared to other studies.More attention should be paid to HBV vaccination and risk-lowering activities.

  10. [Prevention of hepatitis B--HLA DR/DQ in HBs Ag nonresponder].

    Science.gov (United States)

    Ouchi, E; Hoshino, A; Miura, T; Nagahara, N; Kudo, Y; Tamura, M

    1989-11-01

    To evaluate the immunological background in HBs Ag nonresponders against hepatitis B vaccine, the lymphocyte surface marker and HLA-DR/DQ antigen were determined on hospital personnel, 70 males and 256 females, injected hepatitis B vaccine for three times. The vaccine made from the plasma of HBs Ag carriers was injected at the first and second vaccination and recombinant hepatitis B vaccine was injected at the third vaccination. A month after the third vaccination, blood was withdrawn for HBs antigen test by RIA. Border line cases (cut off index 1.0-1.9) are included in nonresponder (cut off index less than 0.9). Both nonresponders and low responders (cut off index 2-49) are more often seen in males than females and high responder (cut off index 50 or more) are seen more often in young females than males. Lymphocyte surface markers were studied by flow cytometry using the following monoclonal antibodies; OKT 3, 4, 8, DR, NK, Ia 1 and B7. No differences between lymphocyte surface markers of nonresponders and responders were noted. HLA-DR/DQ antigens were studied by the cytotoxicity test using Locus DR/DQ, Terasaki Second DR W-60 Tray and using following antibodies; DR 1, DR 2, DRW 15, DR 4, DR 5, DR 7, DR 9, DRW 10, DRW 8, DRW 12, DRW 13, DRW 6, DRW 52, DRW 53, DQW 1, DQW 6, DQW 2, DQW 3, DQW 7 and DQW 4. No significant differences between HLA-DR/DQ of nonresponders and responders were noted. PMID:2601078

  11. Anticuerpos antiprotrombina y resistencia adquirida a la proteína C activada en el síndrome antifosfolipídico

    OpenAIRE

    Muñoz Rodríguez, Francisco José

    2003-01-01

    El síndrome antifosfolipídico (SAF) se caracteriza clínicamente por la aparición de fenómenos trombóticos recurrentes, complicaciones obstétricas, tales como abortos o pérdidas fetales recurrentes, y trombocitopenia. Inmunológicamente se define por la presencia de los anticuerpos antifosfolipídicos (AAF), un grupo heterogéneo de autoanticuerpos que reconocen complejos constituidos por fosfolípidos de membrana y proteínas plasmáticas que actúan como cofactores. Los mejor conocidos y que ayudan...

  12. Detección de anticuerpos contra Borrelia burgdorferi e identificación de garrapatas ixodidas en Piura Y Amazonas, Perú

    OpenAIRE

    Martha Glenny A; Leonardo Mendoza U; Eduardo Falconí R

    2004-01-01

    Objetivos: Detectar anticuerpos IgG/IgM contra Borrelia burgdorferi en población general, procedentes de los departamentos de Piura y Amazonas e identificar especies de garrapatas probablemente incriminadas en la transmisión de la enfermedad de Lyme. Material y Métodos: Entre agosto del año 2001 y junio de 2002, se colectaron muestras de sangre de 232 pobladores procedentes de ocho localidades del Departamento de Piura y 12 del Departamento de Amazonas, para evaluar mediante ELISA Captia™ Lym...

  13. Expression of Hepatitis B virus surface antigen (HBsAg from genotypes A, D and F and influence of amino acid variations related or not to genotypes on HBsAg detection

    Directory of Open Access Journals (Sweden)

    Natalia M. Araujo

    2009-08-01

    Full Text Available The impact of hepatitis B virus (HBV genotypes on the sensitivity of surface antigen (HBsAg detection assays has been poorly investigated. Here, plasmids carrying consensus or variant coding sequences for HBV surface proteins from genotypes A, D and F, were constructed. HBsAg levels were evaluated in medium and extracts of transfected CHO cells by a commercial polyclonal-based assay. We show that HBsAg detection values of consensus forms from genotypes D and F were, respectively, 37% and 30% lower than those obtained by genotype A. However, the presence of two single variations, T143M in genotype A, and T125M in genotype D, produced a decrease of 44% and an increase of 34%, respectively, on HBsAg mean values in comparison with their consensus forms. In conclusion, HBsAg detection levels varied among HBV genotypes. However, unique amino acid substitutions not linked to genotypes, such as T125M and T143M described here, should have more implications in HBV immunological diagnostics than the set of variations characteristic of each HBV genotype.

  14. Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg

    Directory of Open Access Journals (Sweden)

    Jackson Marcondes

    2008-12-01

    Full Text Available The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L. using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety "Vitória de Verão" genetically modified.

  15. Immunostimulatory activities of dendritic cells loaded with adenovirus vector carrying HBcAg/HBsAg

    OpenAIRE

    Jia, Hongyu; Li, Chunling; Zhang, Yimin; Yu, Liang; Xiang, Dairong; Liu, Jun; Chen, Fengzhe; Han, Xiaochun

    2015-01-01

    Objective: This study is to investigate the immunostimulatory activities of dendritic cells (DCs) transfected with HBcAg and/or HBsAg recombinant adenovirus (rAd). Methods: DCs were transfected with rAd (DC/Ad-C+Ad-S, DC/Ad-C, and DC/Ad-S), or pulsed with HBcAg antigen (DC/HBcAg). Flow cytometry was used to detect the phenotype of DCs and the cytokine production of T lymphocytes. Mice were vaccinated with DCs transfected with rAd or pulsed with antigen, and DNA vaccine. Mixed lymphocyte react...

  16. ELISA测定HBsAg室内质控问题的探讨

    Institute of Scientific and Technical Information of China (English)

    范秀芹; 林凌云; 岳志刚; 李爱华

    2002-01-01

    @@ 室内质控是采用一系列的方法连续评价检测过程的稳定性,并保证检测结果的可靠性.目前,ELISA测定HBsAg(还有其他一些测定)结果以定性方式报告,本文对可否采用Levay-Jennings质控图及Westgard多规则进行探讨[1],报告如下.

  17. Estudio de señales de direccionamiento vacuolar del receptor AtRMR1 y su aplicación en la expresión de un anticuerpo recombinante en plantas

    OpenAIRE

    Scabone, Camila

    2012-01-01

    Existe una creciente necesidad de desarrollo de plataformas de producción, a gran escala y de bajo costo, de anticuerpos completos, de manera de satisfacer sus demandas en los distintos tipos de aplicaciones, terapéuticas o diagnósticas. Las plantas son potencialmente el sistema de producción más económico, sin embargo todavía hay que superar varios obstáculos, entre ellos la degradación proteolítica que impacta sobre la calidad y los niveles de acumulación de los anticuerpos producidos. En e...

  18. Prevalencia de anticuerpos contra dengue y leptospira en la población de Jáltipan, Veracruz Prevalence of dengue and leptospira antibodies in the state of Veracruz, Mexico

    OpenAIRE

    Joel Navarrete-Espinosa; Juan Antonio Acevedo-Vales; Emilia Huerta-Hernández; Jorge Torres-Barranca; Dolores Guadalupe Gavaldón-Rosas

    2006-01-01

    OBJETIVO: Conocer la prevalencia de anticuerpos contra dengue y leptospira en Jáltipan, Veracruz, México MATERIAL Y MÉTODOS: Estudio transversal en 500 habitantes de Jáltipan, Veracruz, mediante un muestreo por hogares, aplicación de cuestionario y toma de muestras sanguíneas. Se buscaron anticuerpos IgG y se evaluaron factores asociados a sus seroprevalencias mediante regresión logística RESULTADOS: La seroprevalencia global para denguevirus fue 79.6% (IC95% 76-81); los principales factores ...

  19. A thirty-one year prospective follow-up program on the HBsAg carrier state and primary liver cancer in Qidong, China%乙型肝炎病毒感染与肝癌发生的31年随访研究

    Institute of Scientific and Technical Information of China (English)

    陈建国; 陆建华; 朱源荣; 朱健; 张永辉

    2010-01-01

    Objective To study the relationship between hepatitis B virus (HBV) and primary liver cancer (PLC), and to assess the outcome of PLC in the carriers of HBsAg. Methods General population over age of 15 from a community in Qidong was screened for HBsAg, anti-HBs,and alanine transaminase (ALT) in 1976, and followed-up thenceforth. From January 1, 1977 through December 31, 2007, 12 351 people were enrolled in the cohort, and their occurrence, outcome of PLC and other cancers, together with all the withdrawals due to death were linked to and checked with database from Qidong Cancer Registry and Qidong Vital Registry programs. Results The total observed person-years (PYs) were 355 305.0. One hundred and seventy-three PLC cases were identified among the HBsAg carriers, with an incidence of 361.55 per 100 000 PYs, while PLC cases were only 95 for the non-carriers, with an incidence of 30.90 per 100 000 PYs. The overall relative risk (RR)was 11.70(95%CI: 9.06-15.19) ,with RR 12.30 for men and 10.46 for women. HBsAg carriers had high incidence at each age group,compared with the non-carriers for both men and women. Data from cross-over analysis showed that the incidence rates of PLC for the sub-cohorts of female non-carriers,male non-carriers, female carriers, and male carriers were 1.00,3.07, 10.46, and 37.76, respectively.The cumulative rates of PLC in the 4 groups were 0.86%, 2.73%, 10.22%, and 34.19%, respectively.Results from aon-conditional logistic regression model showed that the gender (male), age, HBsAg ( + ), and ALT( + ) were risk factors for the development of PLC while anti-HBs( + ) demonstrated a protective effect. No relationship was found among carriers and non-carriers for cancer sites such as lung, stomach, esophagus, intestine, pancreas, breast, cervix, bladder, and lymphoma, brain tumor, or leukemia. Conclusion Causation and its strength, together with specificity and persistency of the association were confirmed from this HBsAg-related cohort study in

  20. Anticuerpos antinucleares, imágenes y características obtenidas por inmunofluorescencia: Importancia de los isotipos IgA, IgM e IgG Antinuclear antibodies, patterns and characteristics obtained by immunofluorescence: The importance of the IgA, IgM and IgG isotypes

    Directory of Open Access Journals (Sweden)

    Miriam Arcavi

    2009-10-01

    Full Text Available La técnica de elección para el screening de anticuerpos antinucleares (ANA es la inmunofluorescencia indirecta que utiliza como sustrato una línea de células epiteliales de carcinoma de laringe humano (IFI-HEp2, y como antisuero, anti-IgG o anti-Ig totales. Los ANA-IgG son los más importantes para el diagnóstico y monitoreo de las enfermedades del tejido conectivo (ETC, mientras los ANA-IgM son de menor relevancia clínica en estos pacientes. Sin embargo, poco se sabe de los ANA-IgA ya que estos Ac han sido menos investigados. El objetivo de este trabajo fue estudiar la prevalencia de los diferentes isotipos de inmunoglobulinas de anticuerpos antinucleares en los pacientes con ETC y evaluar la conveniencia de utilizar conjugados monovalentes o polivalentes. Se procesaron 100 sueros de pacientes con diversas ETC empleando IFI-HEp2, en los cuales se detectó 38% de ANA-IgA (títulos ≥ 1:80 y 12% de ANA-IgM (títulos ≤ 1:160. En 29 casos se detectó IgA en ausencia de IgM, en 3 casos IgM en ausencia de IgA. En todos los casos los ANA-IgG estuvieron presentes. En 6 sueros se observó un cambio de imagen con conjugado anti-IgA y en 3 con conjugado anti-IgM. Debido a la alta prevalencia de ANA-IgA detectada por IFI-HEp2, se destaca la conveniencia de utilizar conjugado anti-Ig totales en lugar de anti-IgG, mientras se desconozca la relevancia de los ANA-IgA en el diagnóstico, pronóstico y seguimiento de las enfermedades reumáticas sistémicas.The indirect immunofluorescence with epitelial cell line from human laryngeal carcinoma as substrate (IIF-HEp2 and anti-IgG or anti-total Ig as antisera, is the technique currently used for the detection of antinuclear antibodies. The most important antibodies for the diagnosis and follow-up of connective tissue diseases (CTD are the IgG-ANA, while the IgM-ANA have no clinical relevance. However the IgA-ANA have not been thoroughly investigated so far. The aim of this work was to study the prevalence

  1. [Asymptomatic carriers of HBsAg: is a follow-up necessary?].

    Science.gov (United States)

    Vignote, M L; Gómez-Camacho, F; Poyato, A; González, R; Palomo, D; Hervás, A; Peña, J; Miño, G

    1995-10-01

    We evaluated the clinical and epidemiological data of 142 HBsAg carriers. This prospective trial is part of a program of study and follow-up in HVB patients. The median age was 34.58 years old, males 56.3%. The average follow-up was 32.4 months. Complete clinical history, routine analysis, liver function tests, alfa-fetoprotein, serology for HVB, HCV and HDV and abdominal ecography were done in all patients. DNA-HVB was done only in special cases. Patients with less than 6 months of follow-up were excluded. The 118 remaining carriers were classified into two groups, depending on ALT values. Group 1 (normal ALT): included 98 carriers, 3 of them developed an active chronic hepatitis that was treated with interferon. A small CHC was diagnosed in another patient and it was resected. Group 2 (elevated ALT): included 20 carriers, only 9 of them agreed to biopsy and we found severe hepatic lesions in 4 of them. No group presented coinfection with HCV or HDV. No patient died. We conclude that the study and follow-up of asymptomatic HBsAg carriers permits an early diagnosis and treatment of the complications of this pathology (chronic hepatitis, CHC, etc); in our study, three patients developed chronic hepatitis, successfully treated with interferon, and one small size CHC was diagnosed in another patient. The study of relatives permits also detect subclinic liver disease and facilitates vaccination to prevention transmission of this infection. PMID:8519537

  2. Treatment of 8 civil pilot HbsAg positive cases%民航飞行员HBsAg阳性治疗八例

    Institute of Scientific and Technical Information of China (English)

    沈燕; 陈晓霞

    2001-01-01

    @@ 一、临床资料 8例患者均为A-320男性飞行员,年龄38~52岁,飞行时间14 000~20 000 h.有HBsAg阳性病史7~11年,历年空勤体检肝功能均正常,一般情况好,未作治疗,体检结论:飞行合格.但要求进行定期观察,实行餐具隔离.1996年检查发现,其中4例无变化,1例伴有HBeAg阳性,3例伴乙型肝炎病毒DNA(hepatitis B virus DNA, HBV-DNA)阳性.因HBeAg及HBV-DNA阳性,提示血液中有HBV复制,具有传染性和(或)肝脏病变呈活动性,故让4例患者暂时停飞,到医院作抗HBV治疗.采用干扰素(美国先灵葆雅中国有限公司生产的基因工程α-2b干扰素)肌肉注射,

  3. Detección de anticuerpos contra Borrelia burgdorferi e identificación de garrapatas ixodidas en Piura Y Amazonas, Perú

    Directory of Open Access Journals (Sweden)

    Martha Glenny A

    2004-01-01

    Full Text Available Objetivos: Detectar anticuerpos IgG/IgM contra Borrelia burgdorferi en población general, procedentes de los departamentos de Piura y Amazonas e identificar especies de garrapatas probablemente incriminadas en la transmisión de la enfermedad de Lyme. Material y Métodos: Entre agosto del año 2001 y junio de 2002, se colectaron muestras de sangre de 232 pobladores procedentes de ocho localidades del Departamento de Piura y 12 del Departamento de Amazonas, para evaluar mediante ELISA Captia™ Lyme IgG/IgM (Trinity biotech la presencia de anticuerpos contra Borrelia burgdorferi. Además, se colectaron garrapatas en animales domésticos por búsqueda directa. Resultados: Se detectó seropositividad en 9,9 % de los sueros evaluados. Asimismo, de 433 garrapatas colectadas se identificaron los géneros: Ixodes (5,5%, Amblyomma (18,0%, Rhipicephalus (23,5%, Anocentor (31,1% y Boophilus (21,7%. Conclusiones: Existen personas seropositivas por Borrelia en Piura y Amazonas, coincidiendo con los hallazgos realizados en Sapillica en el año 1992, además se detectó la presencia de garrapatas del género Ixodes en Piura.

  4. 医用臭氧自体血回输对慢性乙型肝炎患者 HBeAg、HBsAg 及 FibroScan 的影响%Effect of medical ozone autologous blood transfusion on FibroScan, HBsAg and HBeAg in patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    康海燕; 董江龙; 李健; 陈蕾; 叶立红; 王艳; 王晓静; 王晨雪

    2015-01-01

    目的:观察医用臭氧对慢性乙型肝炎患者HBeAg、HBsAg及瞬时肝弹性测定(FibroScan)的影响,评估医用臭氧在慢性乙型肝炎患者中的抗病毒、抗纤维化作用。方法将120例HBeAg阳性的慢性乙肝患者随机分成2组,治疗组给予医用臭氧自体血回输治疗(采集患者静脉抗凝全血100 mL,在其中缓慢注入100 mL 30μg/mL医用臭氧,然后再回输到静脉),每周3次;对照组给予聚乙二醇干扰素α-2 a 180μg皮下注射,每周1次。观察2组治疗4周、8周、12周时血清HBsAg、HBeAg定量及FibroScan的变化。结果2组在治疗4周、8周、12周时血清HBsAg、HBeAg定量及FibroScan均逐渐下降(P均<0.05);2组间各指标比较差异无统计学意义(P均>0.05);患者血清HB-sAg定量、HBeAg定量与FibroScan之间呈正相关(r=0.764,0.779,P均<0.05)。结论医用臭氧自体血回输治疗慢性乙型肝炎与聚乙二醇干扰素α-2a效果相当,其抗病毒、抗纤维化效果确切。%Objective It is to observe the influence of medical ozone on HBeAg , HBsAg and transient liver elasticity ( Fi-broScan ) in patients with chronic hepatitis B , and evaluate the antiviral and anti fibrosis effects of medical ozone in the treat -ment of chronic hepatitis B patients .Methods 120 patients with HBeAg positive chronic hepatitis B were randomly divided in-to two groups.The patients in the treatment group was treated with medical ozone autologous blood transfusion (anticoagulant venous blood 100 mL was collected from patients , which was slowly injected into the 100 mL 30μg/mL of medical ozone , and then returned to the vein ) , three times a week;the patients in the control group were treated with the polyethylene glycol inter -feron α-2a 180 μg by subcutaneous injection , once a week.The changes of serum HBeAg , HBsAg quantification and Fi-broScan value in the treatment of 4 weeks, 8 weeks, and 12 weeks were

  5. Eficacia experimental de anticuerpos IgY producidos en huevos, contra el veneno de la serpiente peruana Bothrops atrox Experimental efficacy of IgY antibodies produced in eggs against the venom of the Peruvian snake Bothrops atrox

    Directory of Open Access Journals (Sweden)

    Julio C. Mendoza

    2012-03-01

    Full Text Available Objetivos. Desarrollar un protocolo de inmunización para producir inmunoglobulinas IgY de origen aviar contra el veneno de la serpiente peruana Bothrops atrox y evaluar la capacidad neutralizante. Materiales y métodos. Se inmunizaron seis gallinas de postura de la raza hy line brown con 500 μg/dosis de veneno de B. atrox en un periodo de dos meses. Cada semana, los huevos fueron colectados para el aislamiento de inmunoglobulinas IgY a partir de la yema, usando dos pasos consecutivos con αcido caprνlico y sulfato de amonio. La detecciσn de anticuerpos se realizσ por inmunodifusiσn doble mientras que el tνtulo y reactividad cruzada se determinaron por las técnicas de ELISA y Western blot. El cálculo de DL50 y de la DE50 del antiveneno IgY producido se realizó utilizando el método de Probits. Resultados. La masa de anticuerpos aislados fue de 8,5 ± 1,35 mg de IgY/mL de yema. Asimismo, la DE50 del antiveneno aviar fue calculada en 575 μL de antiveneno/mg de veneno. Adicionalmente, los ensayos de reactividad cruzada mostraron que el veneno de B. atrox comparte mas epνtopes comunes con el veneno de B. brazili (47% que con otros veneno del mismo género, en tanto que los venenos de Lachesis muta (19% y Crotalus durissus (12% mostraron una baja reactividad cruzada. Conclusiones. Se ha obtenido IgY purificada contra el veneno de B. atrox con capacidad neutralizante y se ha demostrado su utilidad como herramienta inmunoanalítica para evaluar la reactividad cruzada con venenos de otras especies.Objectives. To develop an immunization protocol in order to produce avian IgY immunoglobulins against Bothrops atrox Peruvian snake venom and to evaluate its neutralizing capacity. Materials and methods. Six Hy Line Brown hens were immunized each two weeks using 500μg/doses of B. atrox venom in a period of two months. Each week, eggs were collected for IgY isolation from yolk using two consecutive steps with caprilic acid and ammonium sulfate

  6. 制备源自HBsAg基因修饰树突状细胞的外切体%Generation of exosomes derived from adenovirus-mediated HBsAg gene-modified dendritic cells

    Institute of Scientific and Technical Information of China (English)

    杨静悦; 高琳; 付蓉; 薛妍; 刘文超

    2012-01-01

    Objective: To obtain exosomes derived from adenovirus - mediated HBsAg gene - modified dendritic cells. Methods: Full length HBsAg cDNAs were cloned into shuttle2 vector. The HBsAg gene fragments resulted from the - S digested with PI - See and I - Ceu were linked to the linear adeno - X virus DNA. After packaged with HEK293 cells, the adenovirus expression vector was obtained. Then the recombinant adenovirus expression plasmid AdVHBsAg was transfected into human monocyte - derived dendritic cells. The exosomes were isolated from superna-tant of transfected DCs. Transmission electron microscopy was used to observe their structures. The expressions of several proteins were investigated by flow cytometry. Results: The shuttle2 - S showed that band with 630 bp by di-gested with PI - See and I - Ceu, HBsAg gene in the inserted DNA of AdVHBsAg was confirmed by PCR, and pre-dictive fragments proved by restriction enzyme digestion analysis were exhibited. CPE appear 10 after days HEK293 cells transfected AdVHBsAg. Application of the isolation procedure to transfected DCs revealed exosome vesicles by transmission electron microscopy. Protein analysis by Western blot was performed and revealed that the costimulatory molecule CD86,CD83 and HBsAg was detectable. Conclusion; The exosomes derived from HBsAg - DC may be a tool of the HBV related hepatocellular carcinoma immunotherapy.%目的:制备一种新型负载HBsAg基因的外切体(exosome)瘤苗,并探讨其生物学特性、免疫学功能.方法:运用分子克隆和病毒载体转染HBsAg基因构建AdVHBsAg-DC肝癌瘤苗,采用流式细胞术鉴定转染基因表达;提取exosome;以透射电镜观察、Western blot法鉴定exosome.结果:构建的重组AdVHBsAg腺病毒载体,经PCR和酶切鉴定,结果显示HBsAg基因片段已正确插入腺病毒载体中.包装的腺病毒载体具有良好的感染性,可以在293细胞中形成病毒颗粒.提取的exosome在透射电镜下可观察到直径为50-100nm

  7. Expression of a single-chain trimer of MHC restricted HBsAg CTL epitope using adenovirus vector containing GFP-report gene%采用绿色荧光蛋白腺病毒载体表达HBsAgCTL优势表位肽MHC单链三聚体

    Institute of Scientific and Technical Information of China (English)

    陈心春; 刘威龙; 杨桂林; 刘勇军; 朱秀云; 张红梅; 周伯平; Lybarger Lonnie

    2009-01-01

    目的 采用携带GFP报告基因腺病毒载体表达HBsAg细胞毒性T细胞(CIL)表位MHC单链三聚体,为增强HBV特异性免疫提供新方法.方法 构建小鼠MHC Ⅰ类分子(H-2Ld)限制的HBsAg优势CTL表位肽与MHCⅠ类分子重链(Ld)和β2M链(β2-microglobulin,β2微球蛋白)的单链三聚体(HBsAg-SCT).并将HBsAg-SCT亚克隆到GFP标记腺病毒载体,以HBsAg和OVA-SCT作为对照,转染293A细胞,包装产生携带HBsAs-SCT,HBsAg和OVA-SCT的重组腺病毒.结果 经双酶切和克隆测序鉴定,HBsAg-SCT能成功克隆到编码GFP标记的腺病毒载体,通过荧光显微镜观察到转染的293A细胞含有绿色荧光素蛋白.通过Western Blot进一步证实表达的重组蛋白HBsAg-SCT能与抗-腿抗体发生反应.结论 编码HBsAg-SCT的荧光蛋白腺病毒载体成功构建,并能在293A细胞中完整包装成重组腺病毒颗粒.%Objective To generate a recombinant Adenovh'us encoding a GFP (green fluorescent protein)-report gene and a single-chain trimer of MHC restricted HBsAg CTL epitope. Methods An oligonucleotide encoding H-2Ld restricted HBsAg CTL epitopo was synthesized and fused with H-2Ld DNA molecule to construct the eukaryotic expression vector carrying the HBsAg-SCT gene. The HBsAg-SCT gene was subcloned into a GFP adenovirus expression vector,which was transfeeted into Ad293 cells for packaging and amplification of recombinant adenovirus encoding HBsAg-SCT. Results HBsAg-SCT has been cloned into an adenovirus vector encoding GFP report gene successfully as confirmed by double enzyme digestion and direct sequencing. HBsAg-SCT was expressed by infected Ad293 ceils demonstrated by western blot assay. Conclusion A recombinant adenovirus expressing HBsAg-SCT and green fluorescent protein report gene has been generated.

  8. Estudio del perfil de anticuerpos contra Helicobacter pylori producidos por pacientes con diferentes patologías gastroduodenales

    Directory of Open Access Journals (Sweden)

    Spinel Clara

    2004-12-01

    Full Text Available La colonización de la mucosa gástrica con Helicobacter pylori causa la infección bacteriana más frecuente a nivel mundial que es la mayor causa de enfermedades gastrointestinales en humanos. Con el objetivo de examinar el reconocimiento de antígenos de dos cepas de la bacteria en
    150 pacientes infectados y con inflamación (30 de gastritis crónica no atrófica G, úlcera duodenal (30 UD, patologías preneoplásicas (30 de gastritis atrófica GA y 30 de metaplasia intestinal MI y adenocarcinoma gástrico (30 C, se realizó un sistema de cultivo de Helicobacter pylori
    con células epiteliales gástricas. Los sobrenadantes de estos cocultivos fueron empleados como preparación antigénica en pruebas de inmunotransferencia, en las que se detectaron los anticuerpos
    IgG totales, al igual que sus cuatro subclases (IgG1, IgG2, IgG23, IgG4 en los sueros de los pacientes. Se encontró que los perfiles antigénicos de las dos cepas de Helicobacter pylori, aisladas de un paciente con úlcera duodenal y uno con adenocarcinoma gástrico, eran semejantes, con 23 proteínas comunes que incluían los principales factores de virulencia de la bacteria (CagA, VacA, Ureasa, Flagelina. Aunque los sueros mostraron un reconocimiento variado de cada uno de los antígenos, no se encontraron diferencias entre el número de antígenos reconocidos según la patología ni la cepa, tanto para las IgG totales (p = 0,98, como cada una de las subclases (IgG1:p = 0,97; IgG2: p = 0,72; IgG3: p = 0,61; IgG4: p = 0,84. Con el fin de analizar simultáneamente la respuesta de los pacientes hacia el total de los 23 antígenos, se aplicaron análisis multivariados gracias a los cuales se distinguieron cuatro proteínas, de 70,
    82, 90 kDa y VacA de 86 kDa como los principales antígenos implicados en la respuesta inmune de las personas infectadas. Adicionalmente, se diferenciaron dos grupos de patologías según el reconocimiento del conjunto de antígenos: por

  9. Seroprevalence of hepatitis B e antigen (HBe antigen and B core antibodies (IgG anti-HBcore and IgM anti-HBcore among hepatitis B surface antigen positive blood donors at a Tertiary Centre in Nigeria

    Directory of Open Access Journals (Sweden)

    Akinbami Akinsegun A

    2012-03-01

    Full Text Available Abstract Background Hepatitis B virus (HBV is a common cause of liver disease throughout the world. HBV is transmitted through blood and other body fluids, including semen and saliva. Chronic replication of HBV virons is characterized by persistence circulation of HBsAg, HBeAg and HBV DNA; usually with anti-HBc and occasionally with anti-HBs. Aim: To determine the prevalence of HBeAg, IgG anti-HBcore and IgM anti-HBcore amongst HBsAg positive blood donors. These parameters are reflective of transmissibility and active hepatitis B infection. A cross sectional study was carried out at the blood donor clinics of Lagos State University Teaching Hospital Ikeja and Lagos University Teaching Hospital Idiaraba. A total of 267 donors were recruited to determine HBe antigen, IgG and IgM anti-HBcore antibodies amongst hepatitis BsAg positive donors. Five milliliters of blood was collected from those who tested positive to HBsAg screen during donation. The sera were subjected to enzyme linked immunosorbent assay (ELISA. Pearson chi-squared test was used for the analytical assessment. Findings A total number of 267 HBsAg positive blood donors were studied. A seroprevalence of 8.2% (22 of 267 HBeAg was obtained, 4 of 267 (1.5% were indeterminate while 241 (90.3% tested negative. Only 27 out of 267 donors (10.1% tested positive to IgM anti-HBcore, 234(87.6% tested negative, while 6(2.2% were indeterminate. A higher percentage of 60.7% (162 of 267 tested positive to IgG anti-HBcore, while 39.3% (105 of 267 tested negative. Conclusion There is a low seroprevalence rate of HBeAg-positive chronic hepatitis and relatively high IgG anti-HBcore and IgM anti-HBcore rates in South West Nigeria.

  10. Development of a Novel, Ultra-rapid Biosensor for the Qualitative Detection of Hepatitis B Virus-associated Antigens and Anti-HBV, Based on “Membrane-engineered” Fibroblast Cells with Virus-Specific Antibodies and Antigens

    OpenAIRE

    Antonios Perdikaris; Nikos Alexandropoulos; Spiridon Kintzios

    2009-01-01

    A novel miniature cell biosensor detection system for the detection of Hepatis B virus (HBV)-associated antigens and anti-HBV is described. The biosensor is based on “membrane-engineered” Vero fibroblast cells immobilized in an alginate matrix. The membrane-engineering process involved the electroinsertion of anti-HBV specific antibodies (anti-HBs, anti-HBe) or antigens (HBsAg) in the membranes of the Vero cells. The attachment of a homologous antigen to the electroinserted antibody (or, resp...

  11. Frequency of anti-HCV, Hbsag and related risk factors in pregnant women at Nishtar Hospital, Multan

    International Nuclear Information System (INIS)

    Background: Viral hepatitis is a global issue. Among the hepatitis viruses hepatitis B and C are important in South Asia including Pakistan. There are various modes of transmission of these viruses. Vertical transmission is also gaining importance. Antepartum screening for HBV and HCV would help the infected women for appropriate antiviral therapy at appropriate time as well as for taking proper care of the newborns. The present study was designed to see the frequency of HBsAg and anti-HCV in pregnant women at Nishtar Hospital, Multan. Methods: This was a cross-sectional study carried out using non-probability purposive sampling technique. The period of the study was from June 2006 to August 2007. Five hundred (500) pregnant women attending outpatient department of Gynaecology and Obstetrics were included. Informed consent was taken. A specially designed proforma was filled in. Anti-HCV and HBsAg were tested by device method. Data were analyzed on SPSS-11. Results: Out of 500 pregnant women 35 (7.00%) were found to be anti-HCV positive and 23 (4.60%) were positive for HBsAg. Mean age was 26.7+-4.8 years. Majority of the patients 263 (52.60%) were in the age group 26-35 years. 138 (27.60%) women were nulliparous and 282 (56.40%) were para 1-4 and anti-HCV and HBsAg were common in this parity group. Only 80 (16.00%) women were para 5 or more. All anti-HCV and HBsAg positive women were house-wives. Most of them were belonging to rural areas having poor socio-economic status. Among 35 anti-HCV positive women, 20 (57.14%) had history of previous surgery, while 13 (37.14%) had history of multiple injections, 5 (14.28%) received blood transfusion, 4 (11.42%) had ear/nose piercing while tattooing was seen in only 2 (5.71%). Among 23 HBsAg positive women, 10 (43.47%) had history of previous surgery. History of multiple injections was present in 6 (26.08%) patients, 4 (17.39%) patients had history of blood transfusion, tattooing, ear/nose piercing, history of dental

  12. Prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre del IMSS, Orizaba, Veracruz, México Prevalence of antibodies against Trypanossoma cruzi in blood bank donors from the IMSS General Hospital in Onizaba, Veracruz, Mexico

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    Angel Ramos-Ligonio

    2006-02-01

    Full Text Available OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70 y un extracto crudo de epimastigotes. Las muestras fueron obtenidas entre los meses de octubre de 2001 a enero de 2002. RESULTADOS: Los 420 donadores de sangre analizados fueron seronegativos para HBV, HCV, BrA, VDRL y HIV. Después del tamizaje de los 420 donadores, se identificaron dos individuos seropositivos por las pruebas de ELISA, Western blot e IFI, con una seroprevalencia de 0.48%. CONCLUSIONES: En este estudio se muestran evidencias de seropositividad para T. cruzi en donadores de sangre del HGRO, lo que sugiere la existencia de riesgo de contaminación por transfusión sanguínea. Por tal motivo, es necesario aplicar programas para el tamizaje serológico a través de técnicas inmunológicas con alta sensibilidad y especificidad.OBJECTIVE: To estimate the prevalence of antibodies against Trypanosoma cruzi in blood donors from Hospital General Regional (HGRO of the Mexican Institute of Social Security (IMSS per its abbreviation in Spanish. MATERIAL AND METHODS: Between October 2001 and January 2002, blood samples were collected from voluntary donors at the blood bank of the Hospital General Regional of IMSS in Orizaba; Veracruz, Mexico. The samples were assayed for anti-T. cruzi by ELISA, Western blot and IFI, using a recombinant protein (MBP::Hsp70, and crude extract from epimastigotes. RESULTS: A total of 420 blood donors were studied; two of them were seropositive for ELISA, Western blot and IFI, with a seroprevalence of 0.48%. CONCLUSIONS: Some blood donors at the HGRO hospital were seropositive for T

  13. Does the antibody production ability affect the serum anti-Helicobacter pylori IgG titer?

    Science.gov (United States)

    Chung, Hyun Ah; Lee, Sun-Young; Moon, Hee Won; Kim, Jeong Hwan; Sung, In-Kyung; Park, Hyung Seok; Shim, Chan Sup; Han, Hye Seung

    2016-01-01

    AIM To investigate the relationship between serum titers of anti-Helicobacter pylori (H. pylori) immunoglobulin G (IgG) and hepatitis B virus surface antibody (HBsAb). METHODS Korean adults were included whose samples had positive Giemsa staining on endoscopic biopsy and were studied in the hepatitis B virus surface antigen (HBsAg)/HBsAb serologic assay, pepsinogen (PG) assay, and H. pylori serologic test on the same day. Subjects were excluded if they were positive for HBsAg, had a recent history of medication, or had other medical condition(s). We analyzed the effects of the following factors on serum titers of HBsAb and the anti-H. pylori IgG: Age, density of H. pylori infiltration in biopsy samples, serum concentrations of PG I and PG II, PG I/II ratio, and white blood cell count. RESULTS Of 111 included subjects, 74 (66.7%) exhibited a positive HBsAb finding. The serum anti-H. pylori IgG titer did not correlate with the serum HBsAb titer (P = 0.185); however, it correlated with the degree of H. pylori infiltration on gastric biopsy (P < 0.001) and serum PG II concentration (P = 0.042). According to the density of H. pylori infiltration on gastric biopsy, subjects could be subdivided into those with a marked (median: 3.95, range 0.82-4.00) (P = 0.458), moderate (median: 3.37, range 1.86-4.00), and mild H. pylori infiltrations (median: 2.39, range 0.36-4.00) (P < 0.001). Subjects with a marked H. pylori infiltration on gastric biopsy had the highest serological titer, whereas in subjects with moderate and mild H. pylori infiltrations titers were correspondingly lower (P < 0.001). After the successful eradication, significant decreases of the degree of H. pylori infiltration (P < 0.001), serum anti-H. pylori IgG titer (P < 0.001), and serum concentrations of PG I (P = 0.028) and PG II (P = 0.028) were observed. CONCLUSION The anti-H. pylori IgG assay can be used to estimate the burden of bacteria in immunocompetent hosts with H. pylori infection, regardless

  14. Anti-hepatitis B core antigen testing with detection and characterization of occult hepatitis B virus by an in-house nucleic acid testing among blood donors in Behrampur, Ganjam, Orissa in southeastern India: implications for transfusion

    OpenAIRE

    Panigrahi Rajesh; Biswas Avik; Datta Sibnarayan; Banerjee Arup; Chandra Partha K; Mahapatra Pradip K; Patnaik Bharat; Chakrabarti Sekhar; Chakravarty Runu

    2010-01-01

    Abstract Background Occult hepatitis B virus (HBV) infection might transmit viremic units into the public blood supply if only hepatitis B surface antigen (HBsAg) testing is used for donor screening. Our aim was to evaluate the prevalence of occult HBV infection among the HBsAg negative/antiHBc positive donations from a highly HIV prevalent region of India. Methods A total of 729 HBsAg negative donor units were included in this study. Surface gene and precore region were amplified by in house...

  15. HBS-1: A Modular Child-Size 3D Printed Humanoid

    Directory of Open Access Journals (Sweden)

    Lianjun Wu

    2016-01-01

    Full Text Available An affordable, highly articulated, child-size humanoid robot could potentially be used for various purposes, widening the design space of humanoids for further study. Several findings indicated that normal children and children with autism interact well with humanoids. This paper presents a child-sized humanoid robot (HBS-1 intended primarily for children’s education and rehabilitation. The design approach is based on the design for manufacturing (DFM and the design for assembly (DFA philosophies to realize the robot fully using additive manufacturing. Most parts of the robot are fabricated with acrylonitrile butadiene styrene (ABS using rapid prototyping technology. Servomotors and shape memory alloy actuators are used as actuating mechanisms. The mechanical design, analysis and characterization of the robot are presented in both theoretical and experimental frameworks.

  16. HYDRODYNAMICALLY BALANCED SYSTEMS (HBS: INNOVATIVE APPROACH OF GASTRO RETENTION: A REVIEW

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    Dubey Vivek

    2012-06-01

    Full Text Available The objective of writing this review on hydrodynamically balanced systems (HBS was collection of the recent literature. The design of hydrodynamically balanced drug delivery system is base on prolong GI residence time of drug in an area of the GI tract to maximize drug reaching its absorption. Gastrointestinal transit time of orally administered dosage forms are controlled by using gastro retentive drug delivery systems (GRDDS. Need for GRDDS a controlled drug delivery system with prolonged residence time in the stomach. Floating drug delivery systems is one of the important approaches to achieve gastric retention to obtain sufficient drug bioavailability. This delivery system is desirable for drugs with an absorption window in the stomach or in the upper small intestine.

  17. The Method of Confirming HBsAg by MEIA%MEIA确证HBSAg的方法学及其应用评价

    Institute of Scientific and Technical Information of China (English)

    张国祥; 成军; 陆明钢; 金晶; 王国政; 孙长贵; 孙关忠

    2003-01-01

    目的建立简便、快速、价廉的MEIA确证HBsAg的方法.方法采用国产多抗HBsAb及标准阴性血清依据中和试验的原理建立MEIA确证HBsAg的方法.同时采用Abbott确证HBsAg试剂盒分别对80例血清标本进行确证并评价其结果.结果该法能够对在线性范围内的HBsAg"阳性"标本(2.1~300S/N)进行确证,对>300S/N以上的HBsAg"阳性"标本应当进行适当稀释.然后再进行确证;采用两种方法对80例HBsAg"阳性"标本同时进行确证试验,两法符合率为100%.结论该法操作简便、成本低廉、结果易判断,在常规检测中可以替代Abbott确证HBsAg试剂盒.

  18. T- and B-cell responses and previous exposure to hepatitis B virus in 'anti-HBc alone' patients.

    Science.gov (United States)

    Wang, Q; Sachse, P; Semmo, M; Lokhande, M; Montani, M; Dufour, J-F; Zoulim, F; Klenerman, P; Semmo, N

    2015-12-01

    A serologic response to hepatitis B virus (HBV) defined as 'anti-HBc alone' is commonly observed, but its significance remains unclear. This study aimed to define the relationship between 'anti-HBc alone' serostatus and HBV infection, including HBV-specific T- and B-cell memory responses. We enrolled 31 'anti-HBc alone' patients. Total HBV DNA and cccDNA were tested by nested polymerase chain reaction (PCR) analysis in liver samples from 22 'anti-HBc alone' patients vs controls (chronic or resolved HBV infection), followed by HBsAg/HBcAg immunohistochemical (IHC) staining. IFN-γ secretion by HBV-specific T cells was compared in individuals who were 'anti-HBc alone' (n = 27), resolved HBV (n = 21), chronic HBV (n = 24) and 12 healthy controls using enzyme-linked immunospot (ELISpot) assays. An HBsAg-IgG B-cell ELISpot assay was performed in 'anti-HBc alone' patients before and after one dose of recombinant HBsAg vaccine. The majority (23/31, 74.2%) of the 'anti-HBc alone' individuals were co-infected with HCV. Infrequent intrahepatic total HBV DNA (2/22, 9.1%) and cccDNA (1/22, 4.5%) were detected in biopsies; HBsAg and HBcAg IHC staining was negative. HBV-specific T-cell responses were similar between 'anti-HBc alone' individuals and HBV resolvers. Circulating HBV-memory B-cell responses were detected in all 'anti-HBc alone' individuals, consistent with an HBsAg-specific memory pool. After one HBV vaccine dose, increased anti-HBs antibody levels were observed, accompanied by an expansion of HBsAg-specific memory B cells (P = 0.0226). 'Anti-HBc alone' individuals showed HBV-specific T-cell and memory B-cell responses typical of previous viral exposure and protective memory, suggesting a resolved infection. PMID:26075501

  19. DETERMINACIÓN DEL CATABOLISMO DE LOS ANTICUERPOS MATERNOS Y SU INTERACCIÓN CON DIFERENTES PLANES VACUNALES PARA LA ENFERMEDAD DE GUMBORO EN POLLOS DE ENGORDE

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    Robin O. J.

    2006-11-01

    Full Text Available Con el in de evaluar el catabolismo de los anticuerpos maternos y su interferencia con tres planes vacunales diferentes contra la enfermedad de Gumboro se realizó un estudio utilizando pollos de la estirpe Ross 308, divididos en cuatro grupos: grupo 1: control (sin vacuna; grupo 2: vacunado los días 1, 7 y 15; grupo 3: vacunados los días 1 y 12; y grupo 4: vacunado el día 12. En el primer experimento el biológico se suministró en el agua de bebida, y en el segundo se hizo en el pico. Se sacriicaron 15 aves de cada grupo a los 1, 12, 21 y 42 días de edad y se evaluó peso corporal, tamaño y peso de la bolsa de Fabricio, relación peso bolsa/peso corporal (PB/PC, grado de depleción linfoide, presencia de cepas estándar y/o variantes del virus y los títulos de anticuerpos para la enfermedad de Gumboro. El catabolismo de los anticuerpos maternos ocurrió entre los 21 y 28 días de edad. En los grupos vacunados no se evidenció una respuesta inmune activa frente a ningún plan vacunal. La relación PB/PC no indicó atroia de la bolsa en el experimento 1, aunque las lesiones histopatológicas en el último muestreo fueron grado 3; en el segundo experimento solamente el grupo 3 presentó una disminución en la relación PB/PC; las lesiones histopatológicas en el tercer y cuarto muestreos, en todos los grupos, fueron clasiicadas como grado 3 y 4. En los dos experimentos se detectó la presencia de cepas tanto clásicas como variantes y una baja respuesta humoral. Ninguno de los tres planes vacunales conirió adecuada protección a las aves.

  20. DETERMINACIÓN DEL CATABOLISMO DE LOS ANTICUERPOS MATERNOS Y SU INTERACCIÓN CON DIFERENTES PLANES VACUNALES PARA LA ENFERMEDAD DE GUMBORO EN POLLOS DE ENGORDE

    Directory of Open Access Journals (Sweden)

    R. Castañeda

    2006-01-01

    Full Text Available Con el in de evaluar el catabolismo de los anticuerpos maternos y su interferencia con tres planes vacunales diferentes contra la enfermedad de Gumboro se realizó un estudio utilizando pollos de la estirpe Ross 308, divididos en cuatro grupos: grupo 1: control (sin vacuna; grupo 2: vacunado los días 1, 7 y 15; grupo 3: vacunados los días 1 y 12; y grupo 4: vacunado el día 12. En el primer experimento el biológico se suministró en el agua de bebida, y en el segundo se hizo en el pico. Se sacriicaron 15 aves de cada grupo a los 1, 12, 21 y 42 días de edad y se evaluó peso corporal, tamaño y peso de la bolsa de Fabricio, relación peso bolsa/peso corporal (PB/PC, grado de depleción linfoide, presencia de cepas estándar y/o variantes del virus y los títulos de anticuerpos para la enfermedad de Gumboro. El catabolismo de los anticuerpos maternos ocurrió entre los 21 y 28 días de edad. En los grupos vacunados no se evidenció una respuesta inmune activa frente a ningún plan vacunal. La relación PB/PC no indicó atroia de la bolsa en el experimento 1, aunque las lesiones histopatológicas en el último muestreo fueron grado 3; en el segundo experimento solamente el grupo 3 presentó una disminución en la relación PB/PC; las lesiones histopatológicas en el tercer y cuarto muestreos, en todos los grupos, fueron clasiicadas como grado 3 y 4. En los dos experimentos se detectó la presencia de cepas tanto clásicas como variantes y una baja respuesta humoral. Ninguno de los tres planes vacunales conirió adecuada protección a las aves.

  1. Optimization of in vitro HBV replication and HBsAg production in HuH7 cell line.

    Science.gov (United States)

    Cavallone, Daniela; Moriconi, Francesco; Colombatto, Piero; Oliveri, Filippo; Bonino, Ferruccio; Brunetto, Maurizia Rossana

    2013-04-01

    The Gunther's vector-free method (GM), using PCR-amplified full length HBV-DNA (fl-HBV-DNA), is currently the best in vitro HBV replication system despite the low intracellular HBV-DNA production. The replication efficiency and HBsAg secretion of 12 isolates from HBsAg/HBeAg positive sera by GM, Monomer-Linear-Sticky-Ends-DNA (MLSE) and Monomer-Circular-Closed (MCC) were compared in HuH7 cells. Eight of twelve genomes (67%) were replication competent by GM; however direct sequencing (DS) showed that more than 80% of input DNA was undigested in spite of SapI treatment. Replication Intermediates (RI) were detected earlier (24 vs. 48h) and in higher amounts (2.51±0.32 and 6.43±0.43 fold) by MCC than GM or MLSE. By MCC 10 of 12 genomes (83%) were replication competent and 7 produced high RI levels. RI and HBsAg kinetics correlated positively in MCC (R=0.696, p=0.017 overall; R=0.928, p=0.008), but not in GM (R=-0.437, p=0.179 overall; R=-0.395, p=0.439) in genotype D isolates. In conclusion, HBV-DNA circularization prior transfection improves in vitro viral replication and replication competent HBsAg production, mimicking better the in vivo conditions.

  2. Serum Anti-Hbs-Ag in Stable Hemodialysis Patients and its Relationship with Various Demographic and Biochemical Data

    OpenAIRE

    Baradaran, Azar; Ardalan, Mohammad-Reza; Nasri, Hamid

    2010-01-01

    Introduction: To evaluate the relationship between various biochemical, nutritional and demographic factors with immune response to hepatitis B vaccine in maintenance hemodialysis (HD) patients. Material and Methods: A retro-prospective study was carried out on 68 patients undergoing maintenance hemodialysis .Patients were vaccinated against hepatitis B virus with an intramuscular hepatitis B vaccination schedule, 40 micrograms at 0, 1, and 6 months. We also selected 32 age matched normal he...

  3. Detección de anticuerpos contra el virus de la peste porcina clásica mediante la prueba inmunoenzimática (CIV-ELISA y seroneutralización Detection of antibodies against the classical swine fever virus by enzime-linked immunosorbent assay (ELISA

    Directory of Open Access Journals (Sweden)

    A. ISLAS

    1997-01-01

    Full Text Available La peste porcina clásica (PPC es una enfermedad de gran importancia mundial. En Chile está sometida a un programa de control y erradi-cación. El presente ensayo tuvo como objetivo estandarizar y comparar una técnica inmunoenzimática (CIV-ELISA que detecta anticuerpos del tipo IgG dirigidos contra el virus de la PPC, con la seroneutralización (SN, técnica oficial para el diagnóstico serológico. Se utilizaron 20 cerdos híbridos Landrace x Large White de 20 kg de peso vivo, los que fueron inoculados experimentalmente con una dosis de 100.000 DICT50 del aislado virulento Quillota del virus PPC. Un grupo constituido por 4 cerdos fue vacunado 14 días antes de la inoculación. Se tomaron muestras de sangre a los 3, 6, 9, 12 y 14 días post-inoculación. Además, se obtuvieron muestras serológicas de 100 hembras reproduc-toras. Los anticuerpos anti-PPC fueron determinados por un CIV-ELISA policlonal, realizándose comparación con la prueba de SN. La prueba CIV-ELISA no detectó anticuerpos en los cerdos inoculados experimentalmente hasta los 14 días post-inoculación. Sin embargo, los cerdos vacunados previamente fueron seropositivos, situación ratificada por la SN. La sensibilidad y especificidad del CIV-ELISA fue de 90.1% y 76.4%, respectivamente. El CIV-ELISA demostró ser una prueba de fácil aplicación y los resultados son similares a los de la SNClassical swine fever (CSF is an important worldwide disease. In Chile, it is subject to a control and erradication programme. The objective of the present assay was to standardize an immunoenzimatic technique (CIV-ELISA that detects antibodies of the IgG type directed against the CSF virus and to compare it with seroneutralization. Twenty hybrid Landrace x Large White pigs with a live weight of 20 kg were used. They were experimentally inoculated with a dose of 100.000 TCID50 of the Quillota virulent isolate of the CSF virus. A four-pig group was vaccinated 14 days before inoculation

  4. Prevalence of Serologic Hepatitis B Markers in Blood Donors From Puebla, Mexico: The Association of Relatively High Levels of Anti-Core Antibodies With the Detection of Surface Antigen and Genomic DNA

    Directory of Open Access Journals (Sweden)

    Sosa-Jurado

    2016-06-01

    Full Text Available Background The hepatitis B virus (HBV causes chronic hepatitis, hepatic cirrhosis, and hepatocellular carcinoma. Surface antigen (HBsAg detection is a definitive test that can confirm HBV infection, while the presence of antibodies against the core protein (anti-HBc suggests either a previous or ongoing infection or occult hepatitis B infection (OBI. Objectives The aim of the present study was to determine the prevalence of anti-HBc and HBsAg in blood donors. Further, the study aimed to estimate the anti-HBc level at which HBV DNA is detected in putative OBI cases, as well as to search for mutations in the “a” determinant associated with the non-detection of HBsAg in serum. Patients and Methods We conducted a cross-sectional study from 2003–2009. The study included 120,552 blood donors from the state of Puebla, Mexico. Different commercial systems based on microparticles (enzymatic (MEIA or chemiluminescent (CMIA were used to determine the HBsAg and anti-HBc levels. For the detection of HBV DNA, a nested polymerase chain reaction (nested PCR was used and the genotypes were determined using Sanger sequencing. Results Of the 120,552 blood donors, 1437 (1.19%, 95% CI: 1.12 - 1.26 were reactive to anti-HBc, while 82 (0.066%, 95% CI: 0.053 - 0.079 were reactive to HBsAg. Some 156 plasma samples collected in 2009 from anti-HBc-positive/HBsAg-negative blood donors were submitted for HBV DNA detection in a search for probable OBI. Viral DNA was detected in 27/156 (17.3%, 95% CI: 11.5 - 23.1. Our results show an association between HBV DNA or HBsAg and anti-HBc S/CO levels ≥ 4.0. All DNA samples were identified as genotype H and some “a” determinant mutations were identified, although none corresponded to mutations previously reported to hinder the detection of HBsAg by commercial immunoassays. Conclusions We observed that as the anti-HBc levels increase, there is a higher prevalence of the viral protein HBsAg in blood donors. Samples testing

  5. HBsAg and HBeAg Test Analysis of Employees from Food Industry and Recreational Services%南宁市食品及公共场所从业人员HBsAg和HBeAg检测结果分析

    Institute of Scientific and Technical Information of China (English)

    马海芳

    2011-01-01

    Objective To chracterize carriers of hepatitis B virus ( HBV ) in employees of food industry,catering, and recreational services. Methods Serological testing of HBsAg and HBeAg was performed by using enzyme-linked immunosorbent assay ( ELISA ) in 1248 subjects from these sectors. Results Furthermore ,37.22% of HBeAg-positive carriers aged 18-27 years ,27.92% aged 28-37 years,20.22% aged 38-47 years, and 14.64% aged 48-60 years ( x2 = 40.1, P < 0.01 ). No significant differences in gender ratio were identified among age groups except for age group 28-37 years. Conclusion Employees of food industry and recreational services have a high positivity of HBsAg and HBeAg. Monitoring of HBsAg-positive carriers and HBV vaccination are required in addition to HBV awareness education.%目的 了解南宁市从事食品生产、饮食和公共场所从业人员乙型肝炎病毒携带情况.方法 采用酶联免疫吸附试验对1248例从业人员进行血清HBsAg和HBeAg检测.结果 HBeAg构成比的年龄分布最多是18 ~27岁(37.22%),其次是28 ~37岁(27.92%).各年龄组间HBeAg构成比的差异有统计学意义(χ2=40.1,P<0.05).28 ~37岁年龄组男女间HBeAg构成比的差异有统计学意义(χ2=6.25,P<0.05).结论 南宁市食品及公共场所从业人员HBsAg阳性率和HBeAg构成比比较高,应加强对HBsAg阳性人群的监管力度和乙肝疫苗的接种,同时做好乙肝防治知识的宣传.

  6. Multi-copy CpG ODN-containing plasmid enhancing immune responses to HBsAg%含CpG免疫刺激序列的质粒增强HBsAg诱导的免疫应答

    Institute of Scientific and Technical Information of China (English)

    解冰; 陈志辉; 唐紫薇; 劳文光; 李建军; 王岩; 朱诗应; 陶清源; 戚中田; 赵平

    2012-01-01

    Objective To explore the possibility of using plasmid containing muti-copy of CpG ODN as the adjuvant for therapeutic vaccine against hepatitis B. Methods A plasmid pKO-CG6 containing six copies of D type CpG ODN was constructed. The plasmid and the carrier plasmid pKO were used to stimulate peripheral blood monouclear cells (PBMC) of healthy or HBV infected subjects; then the proliferation of PBMCs and secretion of IFN-γ and IL-12 were examined. Recombinant HBsAg combined with either of the plasmid was used to immunize BALB/c mice, and immune responses to HBsAg were assayed. Results Both plasmid pKO-CG6 and carrier plasmid pKO not only effectively activated the proliferation response of PBMCs from healthy controls and HBV infected subjects in vitro, but also promoted the production of IFN-y and IL-12; the immuno-stimulation activity of pK0-CG6 was greatly stronger than that of the carrier plasmid pKO. In vivo study showed that although vector pKO could also act as immunological adjuvant for HBsAg in mice, plasmid pKOCG6 elicited much stronger immune responses to HBsAg, especially the cell-mediated response. Conclusion Plasmid containing multi-copy of CpG ODN can effectively activate PBMCs of HBV infected subjects and enhance the immune responses to HBsAg in mice.%目的 探讨以含有多拷贝CpG寡脱氧核苷酸(CpG ODN)的质粒作为治疗性乙肝疫苗佐剂的可行性.方法 构建含有6个拷贝D型CpG ODN的质粒pKO-CG6,将该质粒以及载体pKO分别刺激健康人及HBV感染者的外周血单核细胞(PBMC),检测PBMC的增殖及分泌的细胞因子IFN-γ、IL-12,进一步将重组HBsAg分别联合这两种质粒免疫小鼠,检测小鼠的细胞和体液免疫应答.结果 质粒pKO-CG6与载体pKO在体外均能有效激活健康人及HBV感染者PBMC增殖反应,并促进IFN-γ、IL-12的产生,其中pKO-CG6的免疫刺激活性强于载体pKO.小鼠体内试验表明,虽然载体pKO也具有免疫佐剂作用,但pKO-CG6更能显著增强HBs

  7. The Comparison of Detection of HBsAg of Blood - donation Volunteers between ELISA Method and Gold Test Strip Method%ELISA法与金标试纸法检测无偿献血者HBsAg的比较

    Institute of Scientific and Technical Information of China (English)

    汪青山

    2010-01-01

    目的 研究金标试纸法与ELISA法检测无偿献血者HBsAg结果的阳性率和漏检率.方法 我站收集的18000份样本分别用金标试纸法与ELISA法测试HBsAg,比较两种方法的阳性率.结果 金标试纸检出的阳性与ELISA法检出的阳性完全相符,另外ELISA法检出的134份阳性标本为金标试纸法漏检.结论 金标试纸法漏检率高于ELISA法.

  8. 健康教育路径对HBsAg阳性产妇母乳喂养依从性的影响%Effects of Health Education Path on Breastfeeding Compliance of HBsAg Positive Puerperae

    Institute of Scientific and Technical Information of China (English)

    尤炜; 轧春妹; 李楠

    2012-01-01

    Objective To investigate the effects of health education path on breastfeeding compliance of HBsAg positive puerperae.Methods Using convenience sampling method, 238 HBsAg positive puerperae from January 2009 to January 201.1 were selected as observational group to receive health education path.263 HBs/Vg positive puerperae from December 2006 to December 2008 were selected as control group to receive, routine health education.The inter-group maternal breastfeeding compliance and awareness of maternal breastfeeding knowledge were compared.Results Compared with the control group, the awareness of maternal breastfeeding knowledge was significantly higher and 70.b9% oi the puerperae gained 80 points or higher( P < 0.01).Compared with the control group, 69.75% of the puerperae' breastfeeding time are more than six months,19.75% are less than six months.only 10.50% without breastfeeding in the observational group, and the differences are significant ( P <0.01).Conclusion Breastfeeding of HBsAg-positive mothers does not increase the HBV infection of infants.Health education path can effectively improve the compliance of maternal breastfeeding and awareness of feeding knowledge.%目的 探讨健康教育路径对HBsAg阳性产妇母乳喂养依从性的影响.方法 便利抽样法选择2009年1月至2011年1月住院分娩的238例HBsAg阳性产妇为观察组,采用健康教育路径方式宣教;选择2006年12月至2008年12月住院分娩的263例HBsAg阳性产妇为对照组,采用传统健康教育方式进行宣教.比较两组产妇母乳喂养依从性及产妇母乳喂养知识知晓情况.结果 观察组产妇对于母乳喂养的相关知识知晓情况较好,得分80分以上的占70.59%,与对照组相比,差异有统计学意义(P<0.001).观察组中,哺乳≥6个月的产妇占69.75%,哺乳<6个月者占19.75%,未采用母乳喂养者仅占10.50%,与对照组相比,差异有统计学意义(P<0.01).结论 HBsAg阳性产妇进行母乳喂养并不增加婴

  9. Anti-HBV prevalence in firstyear students, that will practise professions of high risk of infection with the HBV

    Directory of Open Access Journals (Sweden)

    Ioannis Ioannidis

    2007-10-01

    Full Text Available Aim: We have studied the prevalence of Hepatitis B Virus on the first‐year students of the Paramedical Schools (Medical Lab Department, Nursing Department, Baby Nursing Department and Obstetrics Department of the Health and Foresight Professions School of Technological Educational School of Thessaloniki. Materials and Methods: The sample consists of 502 students of the Paramedical Schools. They were taken serum sample for quantitative determination of HbsAg, Anti-HBs, Anti-HBc. The samples were checked with immunological analyser that uses ELISA method by the manufacturer's criteria. Results: 502 serum samples were checked and 4 samples were found HbsAg positive. A Confirmation Assay used on these samples and showed that truly HbsAg positive was only one sample (percentage ~0,2%. Quantitative determination of Anti‐HBs showed: 175 serum samples had antibody titer >500 MIU/mL, 97 serum samples had antibody titer 101-500 MIU/mL, 78 serum samples had antibody titer 11-100 MIU/mL and 152 serum samples had antibody titer 0-10 MIU/mL. Conclusions: The prevalence of Hepatitis B on our sample is low. However anyone must be careful with Hepatitis B, specially the students of Paramedical Schools and other professionals of high risk professions. Vaccination of high risk groups against HB virus would help to decrease he danger from Hepatitis B.

  10. Significance of application of high-sensitive HBsAg ELISA kits in qualified blood donors determined by routine screening tests%超敏HBsAg ELISA检测试剂在常规检测合格的献血者中应用的意义

    Institute of Scientific and Technical Information of China (English)

    桑列勇; 傅立强; 庄培芬; 方放

    2011-01-01

    Objective;To assess the significance of the application of high - sensitive HBsAag ELISA kits in qualified blood donors determined by routine screening tests. Methods: HBsAg was determined in 8692 blood donors by one imported high - sensitive ELISA kit and two domestical produced ELISA kits, respectively. The results were further confirmed by three nested - PCRs specific for different regions in HBV - DNA and other serologic markers of HBV. Results; 48 out of 8692 blood donors were HBsAg positive. Among them, 32 were detected by Hepanostika? HBsAg ELBA kit, 22 by InTec PRODUCTS kit and 14 by Kehua Bio - engineering kit. The "a" epitope was detected in 40 out of 8692 samples by HBV - DNA analysis. Among those, 13 were determined as HBsAg positive and 8 negative by all three ELISA kits. In another 8 samples, HBsAg could be detected only by high - sensitive Hepanostika? HBsAg ELISA kit. Conclusion: Although the application of high - sensitive HBsAg ELISA kit can significantly decrease the risk of HBV infection in blood transfusion, the possibility of missing detection still remains. Therefore, the current strategy should focus on increasing the sensitivity of ELISA kit and promoting the use of DNA analysis technology. The combination of ELISA determination and DNA analysis can profoundly ensure the safety of blood transfusion.%目的:探讨在无偿献血HBsAg筛查中使用超敏HBsAg ELISA的意义.方法:使用一种进口超敏HBsAg试剂和两种国产HBsAg试剂对8692例无偿献血标本进行平行检测,并使用不同区域的三套巢式PCR和检测乙肝三系其他指标对HBsAg结果进行验证.结果:8692例标本中共检测出HBsAg阳性48例,其中Hepanostika HBsAg ELISA试剂32例,英科新创HBsAg ELISA试剂22例,科华HBsAg ELISA试剂14例,对8692例标本进行HBV-DNA测定,有40例获得a表位基因,其中13例为三种HBsAg ELISA试剂检测均阳性,8例为三种HBsAg ELISA试剂检测均阴性,8例为Hepanostika.HBsAg ELISA试剂检测

  11. Immune responses elicited in hepatitis B virus transgenic mice by CpG-ODN and recombinant HBsAg vaccine%佐剂CpG-ODN与重组HBsAg疫苗联合免疫乙型肝炎病毒转基因小鼠的免疫应答研究

    Institute of Scientific and Technical Information of China (English)

    张卓然; 杨淑凤; 李炎; 郑丛龙

    2006-01-01

    目的以CpG-ODN为佐剂与重组HBsAg(rHBsAg)疫苗合用,研究其对乙型肝炎病毒转基因(HBV Tg)小鼠模型的免疫应答效果.方法 40只HBV Tg小鼠随机分为4组,每组小鼠分别注射rHBsAg疫苗(单用rHBsAg组)、rHBsAg疫苗+CpG-ODN(试验组)、rIFNα-2b(IFN组)、生理盐水(对照组).经多次免疫HBV转基因小鼠,于免疫前、后不同时间采血,动态观察各组小鼠血清中HBsAg量、抗-HBs阳性率和HBV DNA的变化,检测肝组织中HBsAg的表达.检测免疫小鼠的外周血T淋巴细胞亚群和白细胞介素2(IL-2)、IL-12(p70)以及γ干扰素(IFN-γ)的含量,分别检测免疫小鼠的脾细胞增殖和细胞毒性T淋巴细胞(CTL)杀伤功能并计算各组小鼠肝组织活性指数(HAI).结果 rHBsAg组和rHBsAg+CpG组在免疫小鼠后2周100%诱导抗-HBs;rHBsAg+CpG组能显著降低血清中的HBsAg量或使HBsAg转阴,rHBsAg+CpG组肝组织中HBsAg的表达量与血清中一样降低,并降低血清中HBV DNA的拷贝数.rHBsAg组的CD3+、CD4+、CD8+细胞在T细胞中所占百分比,IL-2、IL-12(p70)和IFN-γ的含量以及淋巴细胞特异性增殖和杀伤效应均明显高于对照组(P<0.05).rHBsAg+CpG组与rHBsAg组比较,免疫小鼠产生更强的HBV特异性细胞应答(P<0.05),且以Th1型细胞免疫应答为主.在rHBsAg+CpG组肝组织中出现大量淋巴细胞,肝脏的HAI在4个组中最高.结论 CpG-ODN作为佐剂可以增强重组HBsAg疫苗诱导HBV转基因小鼠产生抗病毒免疫应答,重组HBsAg疫苗辅以CpG ODN可作为免疫治疗慢性HBV感染的可行性途经.

  12. [Prevalence of HBs antigen carriage in a population of national Gendarmerie recruits in Ivory Coast in 2008].

    Science.gov (United States)

    Kra, O; N'dri, N; Ouattara, B; Kadjo, K; Aba, T; Bissagnéné, E

    2012-01-01

    The purpose of this study was to determine the prevalence of HBsAg carriage in Ivorian gendarmerie recruits in 2008. This prevalence was 15.6%. It was highest in recruits aged 18 years (24%), in those who were married (33.3%) and in those who had had more than three sexual partners in the 6 months before the survey (19.4%). It was also higher in recruits who had never used condoms (20.6%) and in those who had undergone blood transfusions (27%). A policy of vaccination against HBV in the army, in teenagers and adults must be implemented to reduce HBsAg carriage in the army and in the Ivorian population.

  13. Bajo índice de sospecha para deficiencia de anticuerpos en niños sometidos a procedimientos quirúrgicos de Otorrinolaringología

    Directory of Open Access Journals (Sweden)

    AB Ramírez-López

    2016-06-01

    Full Text Available INTRODUCCIÓN: las inmunodeficiencias primarias están subdiagnosticadas en todo el mundo, aun en centros de tercer nivel de atención. Los defectos de anticuerpos constituyen el grupo más prevalente y suelen manifestarse clínicamente después de los 6 meses de edad como infecciones respiratorias recurrentes causadas por bacterias encapsuladas. Algunas cirugías de oído-nariz y garganta se indican en pacientes con infecciones respiratorias recurrentes o complicadas que no han respondido adecuadamente al tratamiento médico. Estos niños sometidos a procedimientos quirúrgicos luego de una historia de infecciones respiratorias recurrentes pueden constituir un grupo de alto riesgo para inmunodeficiencias primarias.   OBJETIVO: indagar qué tan frecuentes son los defectos de anticuerpos en niños sometidos a cualquiera de tres cirugías de otorrinolaringología.   MATERIALES Y MÉTODOS: revisamos los expedientes electrónicos de niños que hubieran sido sometidos a adenoamigdalectomía (ATT, drenaje endoscópico de senos paranasales (ESD y colocación de tubos timpánicos de ventilación (TVT en nuestro instituto durante 2011-2012, en busca de niveles séricos de inmunoglobulinas (IgE, IgG, IgA, IgM.   RESULTADOS: encontramos 112 procedimientos quirúrgicos en 87 pacientes, de los cuales la indicación para cirugía fue infecciosa en 37 (21 varones, 1 muerto, edad media 7.3 años, más frecuentemente rinosinusitis (19/37 y amigdalitis (9/37 crónicas. Los procedimientos incluyeron: 24 adenoamigdalectomías, 13 drenajes endoscópicos de senos paranasales y 6 colocaciones de tubos timpánicos de ventilación, para un total de 43; ocho pacientes (21.6% fueron sometidos a más de una cirugía. La IgE sérica se encontró en el expediente de 27 pacientes (72.9%, y “al menos IgG” en 18 (48.6%. Sólo 70% de las pruebas fueron ordenadas antes de la cirugía. Los resultados anormales incluyeron: IgE elevada en 10/27, Ig

  14. 我国研制的乙型肝炎基因重组(CHO)疫苗对乙型肝炎病毒母婴传播阻断效果观察%Study on Protective Efficacy of CHO Expressed Recombinant HBsAg Vaccine in Blocking Mother-Infant Transmission of Hepatitis B Virus

    Institute of Scientific and Technical Information of China (English)

    孟宗达; 韩秀兰; 林惠芳; 杜敏; 侯通; 杨翠丽; 董永辉; 赵玉良; 刘洪斌

    2001-01-01

    为观察我国研制开发的乙型肝炎(乙肝)基因重组(CHO)疫苗阻断乙肝病毒母婴传播的效果,选乙肝表面抗原(HBsAg)、乙肝e抗原(HBeAg)和乙肝核心抗体(抗-HBc)3项均阳性的母亲所生婴儿,用乙肝CHO疫苗免疫,并随访观察3年。婴儿随机分为A组(52例)和B组(50例),A组为单纯疫苗组,注射3次乙肝CHO疫苗(0、1、6个月,10μg/次);B组为疫苗加50IU乙肝免疫球蛋白(HBIG)组,同A组一样注射3次乙肝CHO疫苗,但在0月时同时注射HBIG(50IU)。全程免疫后2~12个月、13~24个月、25~36个月分别采血,以固相放射免疫(RIA)法检测乙肝表面抗体(抗-HBs)、HBsAg和抗-HBc。综合3次随访结果,单独及时全程接种乙肝CHO疫苗组,婴儿抗-HBs阳性率为82.69%,无应答率为3.85%,HBsAg阳性率为13.46%,保护率为83.17%;乙肝CHO疫苗加HBIG组,抗-HBs阳性率为90.00%,无应答率为2.00%,HBsAg阳性率为8.00%,保护率为90.00%。A、B两组的保护率虽不同,但差异无显著统计学意义(χ2=0.77,P=0.3788)。由此可见,乙肝CHO疫苗具有良好的安全性和免疫原性,可有效阻断乙肝病毒的母婴传播,出生时加注50IU HBIG效果更好。%This study was to investigate the preventive efficacy of CHO expressed recombinant HBsAg vaccine in blocking mother-infant transmission of hepatitis B virus. One hundred and two newborns born to HBsAg, HBeAg and anti-HBc positive mothers were randomly divided into two groups. Group A received full-dose HB vaccine at birth, 1 month and 6 months, 10μg/dose, and group B was the same as group A in receiving vaccine but additionally injected 50IU hepatitis B immune globulin(HBIG)at birth. Infants were collected blood 2~12 months, 13~24 months and 25~36 months after full-dose vaccination, and detected anti-HBs, HBsAg and anti-HBc by RIA method. The results were as follows

  15. Evaluation of Domestic Chemiluminescence Immunoassay Kit as a Potential Application in the Detection of HBsAg%国产化学发光免疫试剂检测血清HBsAg的应用评价

    Institute of Scientific and Technical Information of China (English)

    张雄民; 伍晓菲; 刘宇宁; 孟妍; 贾尧; 王迅

    2009-01-01

    目的 考核国产化学发光免疫分析试剂检测血清HBsAg的效果,探讨其在血液筛查和临床检测应用中的可行性.方法 采用国产化学发光免疫分析试剂筛查345份HBsAg确认阳性的血清标本,比较其与常用的进口和国产酶联免疫检测试剂盒的漏检率;并采用不同浓度的标准HBsAg血清对国产化学发光免疫分析试剂进行线性分析和精密性试验.结果 国产化学发光免疫分析检测试剂的漏检率高于Murex和Organon酶联免疫试剂盒,差异有统计学意义(P<0.01),而与国产英科新创(厦门)科技公司和上海科华生物技术有限公司的HBsAg酶联免疫诊断试剂盒的差异无统计学意义;采用标准血清进行线性分析.标本浓度在(0.5~150)ng/ml范围时与化学发光单位量(RLUs)呈良好的线性正相关;分别对低、中、高浓度标准血清进行板内和板间的重复检测,其反应具有良好的重复稳定性.结论 化学发光免疫分析法检测血清HBsAg具有快速、可定量、反应稳定的特点.可用于血液筛查和临床标本的检测.

  16. Tissue localization of C1q in HBs antigen positive liver disease patients by direct immunofluorescent technique

    Directory of Open Access Journals (Sweden)

    Tsuji,Takao

    1977-02-01

    Full Text Available Tissue localization of a subcomponent of the first component of complement (CLq was examined in one postmortem case of HBs antigen (HBs Ag positive hepatocellular carcinoma and in six cases of chronic hepatitis from liver biopsy specimens. The direct immunofluorescent method was used after fixation with 2% para-formaldehyde in concentrated ammonium sulfate. CLq localization was found in collagen fibers and the cytoplasm of fibroblasts in the connective tissues of specimens examined. The localization was particularly marked in the region of the fundal glands of the gastric wall. Apart from collagen fibers, other sites of localization included the surface membrane of lymphocytes, especially those cells of the mesenteric lymph nodes. In HBs Ag positive specimens, immune deposit-like substances appeared localized intra-hepatically and in the renal glomeruli. Since C3 and C4 were identified concomitantly, it indicates that these substances were indeed immune diposits. Despite the finding that C3 and C4 were identified together in the hepatic cell cytoplasm, C1q itself was not demonstrated in all hepatic cell cytoplasms.

  17. Complement fixing hepatitis B core antigen immune complexes in the liver of patients with HBs antigen positive chronic disease.

    Science.gov (United States)

    Rizzetto, M; Bonino, F; Crivelli, O; Canese, M G; Verme, G

    1976-01-01

    One hundred and fifty-two biopsies from serologically HBsAg positive and negative patients with liver disease were studied in immunofluorescence: for the presence of the surface (HBs) and the core (HBc) antigenic determinants foeterminants of the hepatitis B virus, of immunoglobulins and complement (C) deposits, and for the capacity to fix human C. Circumstantial evidence is presented suggesting that HBc immune-complexes are a relevant feature in the establishment and progression of chronic HBSAg liver disease. C fixation by liver cells was shown in all HBC positive patients with chronic hepatitis; an active form was present in every case, except two with a persistent hepatitis, an inverse ratio of HBc to C binding fluorescence being noted between active chronic hepatitis and cirrhotic patients. HBc without C fixation was observed in only three patients in the incubation phase of infectious hepatitis. IgG deposits were often found in HBc containing, C fixing nuclei. No C binding or IgG deposits were observed in acute self-limited type B hepatitis, in serologically positive patients with normal liver or minimal histological lesions, with and without HBs cytoplasmic fluorescence in their biopsy, or in serologically negative individuals. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:1001973

  18. 重组人源性抗HBsAg Fab抗体的肽质量图谱分析

    Institute of Scientific and Technical Information of China (English)

    邓宁; 向军俭; 唐勇; 王宏

    2005-01-01

    重组人源性抗HBsAg Fab抗体具有较好的特异性和抗原结合活性,为了更好的阐明毕赤酵母表达的重组人源性抗HBsAg Fab抗体的性质,用基质辅助激光解析飞行时间质谱(MALDI-TOF-MS)对重组Fab抗体的分子质量和肽质量图谱进行了分析。结果显示,毕赤酵母表达的重组人源性抗HBsAg Fab抗体的分子质量为50678.49Da,与根据其一级结构计算的理论分子质量相比多2763.84Da,显示酵母表达的重组Fab抗体为糖蛋白。用胰蛋白酶酶解重组Fab抗体后进行MALDI-TOF-MS分析显示,大部分的酶解肽段均能检测出来。结果表明毕赤酵母表达的重组Fab抗体与预期的结构一致。

  19. Anticuerpos contra Chlamydophilaen pacientes con infarto agudo del miocardio y riesgo coronario, y su relación con la muerte Antibodies against Chlamydophila in patients with acute myocardial infarction and coronary risk and their association with mortality

    Directory of Open Access Journals (Sweden)

    Guadalupe García-Elorriaga

    2005-06-01

    Full Text Available OBJETIVO: Determinar si los anticuerpos contra Chlamydophila pneumoniae en pacientes con infarto agudo del miocardio y factores de riesgo coronario se asocian con la muerte. MATERIAL Y MÉTODOS: Se hizo un estudio observacional, prospectivo, transversal y comparativo. Se incluyeron en el estudio 100 sujetos que, entre 1999 y 2000, estuvieron hospitalizados en la Unidad Coronaria del Hospital de Especialidades del Centro Médico La Raza, del Instituto Mexicano del Seguro Social. Se trataba de una muestra constituida por pacientes de ambos sexos, mayores de 18 años, con infarto agudo del miocardio y riesgo coronario. Mediante microinmunofluorescencia indirecta se identificaron anticuerpos contra Chlamydophila pneumoniae, Chlamydophila psitacii y Chlamydia trachomatis. De entre los 100 sujetos, se eligieron al azar 33, a quienes se les determinaron anticuerpos contra Chlamydophila, no sólo durante su estancia en el hospital, sino también al salir de éste y a los tres meses de haber sufrido el infarto agudo del miocardio. Se calcularon las medias y las desviaciones geométricas estándares para los títulos de anticuerpos contra Chlamydophila, y se determinó la razón de momios y el intervalo de confianza al 95% entre los factores de riesgo coronario y la muerte. RESULTADOS: Setenta por ciento de los pacientes de la muestra inicial presentaron anticuerpos contra Chlamydophila pneumoniae; no se identificaron anticuerpos contra Chlamydophila psitacii y Chlamydia trachomatis. No se observó una fuerza de asociación estadísticamente significativa con la muerte en pacientes con infarto agudo del miocardio y factores de riesgo coronario. De los 33 individuos de la submuestra, 25 presentaron anticuerpos contra Chlamydophila pneumoniae, y en 83% de estos últimos casos, se registró un descenso de dichos anticuerpos a los tres meses de haberse presentado el infarto agudo del miocardio. CONCLUSIONES: A pesar de que en pacientes con infarto agudo del

  20. HBsAg阴性HBcAb阳性弥漫大B细胞淋巴瘤患者CHOP与R-CHOP方案化疗的肝功能损害分析%Comparative analysis of liver function in HBsAg -/HBcAb + patients with diffuse large B-cell lymphoma treated with CHOP and R-CHOP regimens

    Institute of Scientific and Technical Information of China (English)

    黄燕华; 杨建良; 石远凯; 何小慧; 秦燕; 杨晟; 吕铮; 董梅; 周生余; 刘鹏; 张长弓

    2012-01-01

    目的 分析既往感染乙型肝炎病毒(HBV)的HBsAg(-)HBcAb(+)弥漫大B细胞淋巴瘤(DLBCL)患者在接受CHOP和R-CHOP方案化疗后的肝功能耐受情况.方法 2005年1月至2008年12月收治的86例HBsAg(-)HBcAb(+)DLBCL患者,其中CHOP方案组47例,R-CHOP 方案组39例,均未给予抗乙肝病毒治疗,比较两组患者各周期化疗后及化疗结束后1年内肝功能损害程度.结果 CHOP方案组和R-CHOP方案组在第5个周期化疗后,肝功能损害发生率分别为28.6%和6.2%,差异有统计学意义(P=0.026);而在第1、2、3、4、6个周期化疗后,肝功能损害发生率差异均无统计学意义(均P>0.05).CHOP方案组和R-CHOP方案组的大部分患者中在不同化疗 周期时肝功能正常,均未见Ⅳ度肝功能损害.CHOP方案组和R-CHOP方案组患者在化疗结束后1 ~3个月肝功能正常者分别占 60.0%和68.0%,4~6个月占92.3%和75.0%,7~9个月占90.0%和81.8%,10 ~ 12个月占92.3%和92.9%,差异均无统计学意义(均P>0.05).结论 HBsAg(-)HbcAb(+)的DLBCL患者接受CHOP方案或R-CHOP方案化疗时,肝功能损害发生率均很低,且利妥昔单抗的应用也并未增加患者肝功能损害的发生率,因此本研究不支持常规预防性抗乙肝病毒治疗,但治疗期间应密切监测患者的肝功能、乙肝标志物及HBV-DNA水平.%Objective To analyze the liver function in patients with diffuse large B-cell lymphoma (DLBCL),who are hepatitis B surface antigen negative/antibody to hepatitis B core antigen positive ( HBsAg -/HBcAb + ),treated with CHOP and R-CHOP regimens.Methods In this retrospective study,86 DLBCL patients,who were HBsAg -/HBcAb +,were collected from Cancer Hospital of Chinese Academy of Medical Sciences between January 2005 and December 2008.The patients were given at least two cycles of chemotherapy using CHOP-like or R-CHOP-like regimen without anti-HBV treatment,and followed--up for at least 12 months after completion of

  1. Asociación entre anticuerpos antifosfolípidos y complicaciones de la gestación en mujeres de Costa Rica

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    Roubier Rojas-Barahona

    2007-04-01

    Full Text Available Objetivo: Determinar asociación entre los anticuerpos antifosfolípidos y complicaciones de la gestación (aborto de cualquier tipo, preeclampsia, eclampsia, insuficiencia placentaria grave o parto antes de la semana 34 de gestación, tomando como base la población de pacientes del servicio de Obstetricia del Hospital México. Materiales y métodos: Estudio de casos y controles en la población de pacientes atendidas por el servicio de Obstetricia del Hospital México en el período comprendido entre enero de 2002 y diciembre de 2004. Se tomaron los datos del registro de egresos Hospitalarios, se hizo un análisis descriptivo, en análisis univariado y multivariado de las principales variables registradas. Resultados: El promedio de edad de las pacientes en los casos y los controles no mostró diferencia significativa. La provincia de San José reporta el mayor porcentaje de casos con un 39.9%, seguido por Heredia con un 26.8% y Alajuela con un 24.6%. En cuanto al número de gestaciones, se determinó que no influyen significativamente en relación con el aborto y otras complicaciones de la gestación. El análisis univariado y multivariado mantiene asociación entre los antecedentes clínicos de las gestaciones anteriores, además de los anticuerpos antifosfolípidos respecto al aborto y otras complicaciones de la gestación. Conclusión: Los anticuerpos antifosfolípidos deberían ser tomados en cuenta como parte del seguimiento del embarazo en pacientes con historia de abortos y complicaciones de la gestación en embarazos previos.Aim: To determine the association between antiphospholipid antibodies and pregnancy failures (abortion, preclampsia, eclampsia, placental insufficiency or delivery before the 34th week of gestation in Costa Rican women. Materials and Methods: The present is a case-control study, the participants belong to the Obstetrics Department of the Hospital Mexico. The period of the study was from January 2002 to December

  2. Neosporosis canina: Presencia de anticuerpos sericos en poblaciones caninas rurales y urbanas de Chile Neosporosis canine: detection of sera antibodies in rural and urban canine population of chile

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    A.N. Patitucci

    2001-01-01

    Full Text Available Neospora caninum es un protozoo parásito cuya infección se ha reportado en vacunos, caninos y otras especies en diversas partes del mundo. Los perros actúan como huéspedes definitivos e intermediario a la vez. En esta especie la enfermedad se caracteriza por paresia y/o parálisis de los miembros posteriores y puede ser fatal tanto en cachorros infectados transplacentariamente como en adultos. En Chile, no existe información sobre Neospora en caninos pero se ha demostrado la presencia de anticuerpos contra el protozoo en bovinos, por tal motivo se propuso la identificación de la infección mediante la detección de anticuerpos séricos contra N. caninum en perros. Se estudiaron 201 sueros caninos de diferentes edades, sexo, raza, ambiente ("rural" vs. "urbano" y tipo de alimentación ("carne cruda" vs. "no cruda" en una dilución 1:50 mediante la técnica de Inmuno Fluorescencia Indirecta (IFA. Del total de perros estudiados, el 18% (36/201 presentó anticuerpos contra N. caninum. Además se demostró asociación entre la cantidad de positivos y el ambiente y con el tipo de alimentación; no obstante, no hubo relación con el sexo, raza y edad. Los resultados indican que el pertenecer al ambiente rural y/o consumir carne cruda pueden ser importantes factores de riesgo dentro de la epidemiología de la infección por N.caninum. Complementariamente se procesaron dos sueros de zorros (Pseudolapex fulvipes mediante la técnica del Aglutinación de taquizoitos de Neospora (NAT que resultaron positivos hasta la máxima dilución analizada (1:320, demostrando que los animales estaban infectados al momento de la toma de muestra y abriendo interrogantes en la epidemiología de la enfermedad a nivel local.Neospora caninum is a protozoan parasite whose infection has been detected in canine, bovine and others species around the world. In Chile, the presence of the disease has been already demonstrated in cows of the IX Region of the country. In the

  3. Trend of Hepatitis B Surface Antigen (HBsAg) among blood donors at the blood bank of a tertiary care referral teaching hospital in Southern India

    OpenAIRE

    Yashovardhan A.; Sreedhar Babu K.V.; Jothi Bai D.S.

    2015-01-01

    Background: Blood is a scarce, but lifesaving resource; it is also the most efficient vehicle for the transmission of Hepatitis B virus (HBV). Hence there is a need for accurate screening of hepatitis B surface antigen (HBsAg) among blood donors. The present study was designed to assess the seroprevalence of HBsAg, among the voluntary and replacement blood donors in the blood bank of a tertiary care referral teaching hospital in Andhra Pradesh. Methods: This is a prospective cross sectiona...

  4. 两种不同模式洗板机对ELISA法检测HBsAg结果比较

    Institute of Scientific and Technical Information of China (English)

    徐志强; 钱迎芬; 肖晓晨

    2014-01-01

    目的:比较两种不同模式洗板机(水平式和立式)对酶联免疫吸附试验(ELISA)检测乙型肝炎表面抗原(HBsAg)的洗板技术对检测结果的影响。方法对61例临床确诊为HBsAg阴性和31例临床确诊为HBsAg阳性的标本,经随机排列,用同一批号HBsAg试剂进行检测,分别用手工洗板、anthos fluido水平式洗板机、基波全自动快速立式洗板机洗板,后由酶标仪判读结果(OD值≥0.105为阳性),对阴性数、阳性数进行分析。结果 anthos fluido洗板机洗板5次,假阳性达12例,与标本原始结果相比较(χ2=6.93,p0.05)无差异;手工洗板5次,假阳性4例,与标本原始结果相比较(χ2=2.12,p>0.05)无差异。两种模式洗板机洗板方式对ELISA法检测HBsAg结果(χ2=7.69,p<0.01)有差异。结论在ELISA方法检验当中,立式洗板机比水平式洗板机在洗板时增加了蒸馏水冲洗和脱水的过程,减少了污染和残留,结果更加可靠。

  5. HBsAg腺病毒疫苗载体的构建%THE CONSTRUCTION OF RECOMBINANT ADENOVIRAL VACCINE VECTOR OF HBSAG

    Institute of Scientific and Technical Information of China (English)

    陈枫; 杜光红; 赵新华

    2007-01-01

    目的:构建含HBsAg基因的重组复制缺陷型腺病毒疫苗载体.方法:以pEcob-6为模板,PCR扩增目的基因HBsAg,腺病毒穿梭载体PAd-track-cmv与HBsAg经T4连接酶连接,重组为PAd-track-cmv-HBs穿梭质粒;腺病毒骨架载体质粒PAd-Easy-1再与PAd-track-cmv-HBs在大肠杆菌BJ-5183中同源重组为PAd-Easy-1-HBs质粒.结果:PAd-track-cmv-HBs穿梭质粒经上海基康生物技术公司进行DNA测序,结果与预期结果相同,含有完整的HBsAg目的基因.PAd-Easy-1-HBs用Pac-1酶切,Pac-1能将载体切成两个片段,大片段约为30 kb,小片段约为3 kb.结论:HBsAg腺病毒疫苗载体构建成功,为进一步研究PAd-track-cmv-HBs诱发抗HBV免疫打下了基础.

  6. Determinación de Anticuerpos IgM contra Virus dengue partir de sangre absorbida en papel filtro: Un método alternativo y sencillo

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    María García M

    2000-01-01

    Full Text Available Objetivo: Se propone como alternativa la obtención de muestras de sangre total en papel filtro, para la determinación de anticuerpos IgM contra dengue, por ser un método de recolección sencillo y no requerir de muchos cuidados en el envío al laboratorio. Materiales y métodos: De 100 pacientes con diagnóstico clínico de dengue clásico, se obtuvieron en forma simultánea, muestras de suero en tubos al vacío y de sangre total en papel filtro. Ambas muestras fueron evaluadas con el método serológico ELISA Captura de IgM a los 30 días de obtenida la muestra. Resultados: De las 100 muestras 25 fueron positivas y 75 negativas en suero, y 24 positivas y 74 negativas en papel filtro. Se obtuvo una concordancia por índice Kappa de 0,97, sensibilidad y especificidad de 96,0% y 98,0% respectivamente; el valor predictivo positivo fue 96,0%, y valor predictivo negativo fue 98,0%. Conclusión: Se evidencia muy buena sensibilidad, especificidad y concordancia en la determinación de IgM contra dengue, al utilizar papel filtro en la obtención de muestra de sangre.

  7. Effect of hepatitis B vaccine combined with hepatitis B immune globulin on infection in newborn infants of HBsAg-positive mothers

    Institute of Scientific and Technical Information of China (English)

    Si-Jia Yang; Jin-Hong Liu; Xue-Lian Tong; Ming Chen

    2016-01-01

    Objective:To investigate the effect of hepatitis B vaccine combined with hepatitis B immune globulin on infection in newborn infants of HBsAg-positive mothers. Methods:Two hundred newborn infants of HBsAg-positive mothers who had received prenatal examination and given birth to their child were selected as the study subjects and divided into the control group and the observation group in accordance with the voluntary principle. Newborn infants in the control group received 100 IU of vaccinations of hepatitis B vaccine and hepatitis B immune globulin at postnatal 24 h, 1 month and 6 months, while lying-in women in the observation group were continuously given 200 IU of immune globulin injection at the 27th, 30th, 33rd and 36th weeks of pregnancy. The infection condition and the influence of different delivery modes in infants of the two groups would be observed 12 months after birth. Results:The HBsAg positive rate and HBsAb positive rate of infants in the observation group were 1.00%and 59.00%respectively, while the control group’s were 10.00%and 73.00%. The HBsAg positive rate and HBsAb positive rate of infants in the observation group were significantly lower than those of the control group. The data showed statistical significances. Seven cases of infants born vaginally showed positive HBsAg. One of them belonged to the observation group and the other six were in the control group. Four cases of infants delivered by cesarean section showed positive HBsAg and they all belonged to the control group. The HBsAg positive rate of the observation group was obviously lower than that of the control group no matter what deliver mode those children had received. Conclusions:Hepatitis B vaccine combined with hepatitis B immune globulin could effectively decrease the morbidity rate of positive HBsAg for newborn infants no matter what birth way they had received.

  8. Psychological Health Counselling of HBsAg Carriers among the People Entering or Leaving Fuzhou Frontier Port%福州口岸入出境人员中HBsAg携带者的心理卫生咨询

    Institute of Scientific and Technical Information of China (English)

    高居健; 熊国欢

    1996-01-01

    本文报道了对福州口岸入出境人员在疾病监测体检中所发现HBsAg携带者的异常心理状况.为此,作者针对性提供了心理卫生咨询服务,收到良好的社会效果,为今后更好地开展国际旅行心理卫生保健工作提供了经验.

  9. HBsAg阴性献血者核酸检测阳性结果分析%Positive Analysis of Nucleic Acid Detection for HBsAg Negative Blood Donors

    Institute of Scientific and Technical Information of China (English)

    张冬民; 周小安; 任红红

    2015-01-01

    目的 ELISA检测HBsAg结果阴性的无偿献血者样本进行核酸检测,再对核酸检测阳性结果样本进行“两对半”检测并对献血者追踪检测,分析是窗口期感染还是隐匿型乙型肝炎。方法使用双ELISA试剂检测无偿献血者样本,对检测结果阴性的无偿献血者样本使用浩源核酸筛查系统进行核酸检测,对核酸检测阳性结果样本进行“两对半”检测并对献血者追踪检测。结果 HBsAg(ELISA)阴性献血者血液样本12548份进行核酸检测,结果有13份样本检出HBV DNA,通过“两对半”检测以及对献血者追踪检测,确定2例为窗口期感染,11例为隐匿型乙型肝炎。结论核酸检测应用于血液筛查,防止输血传播疾病有及其重要的意义。%Objective ELISA in the detection of HBsAg negative blood donation sample nucleic acid detection, and of nucleic acid positive test results for samples of "two half and half" detection and the donors track detection, is to analyze the infection in window period or occult hepatitis B. Methods Double ELISA reagent testing samples of unpaid blood donors, negative unpaid donors samples using the result of the test grandsoo DNA screening system for nucleic acid detection, the nucleic acid testing positive results "two half-and-half" samples and blood donors track detection.Results HBsAg (ELISA) negative blood samples of 12548 blood donors for nucleic acid detection, the results of 13 samples detected HBV DNA, by "two half-and-half" detection and track testing of blood donors and to determine 2 cases for the window period, 11 cases of hepatitis b virus infection aslar-pacific perspective. Conclusion Nucleic acid detection applied in blood screening to prevent blood transfusion transmit ed diseases and its important significance.

  10. 化学发光磁微粒免疫分析法定量检测HBsAg

    Institute of Scientific and Technical Information of China (English)

    周齐洋; 金晶; 江波; 先小龙; 颜彬; 吕华; 苏恩本

    2013-01-01

    目的 建立定量检测HBsAg的化学发光磁微粒免疫分析法.方法 2株抗HBs单克隆抗体分别标记吖啶衍生物和生物素化,生物素化的抗HBs通过链霉亲合素包被到磁珠上形成致敏磁珠.将待测标本加入吖啶标记的抗HBs(吖啶-抗HBs)与致敏磁珠的体系中,形成致敏磁珠-HBsAg-吖啶标记抗HBs夹心复合物,磁性分离清洗.重复加样1次,磁性捕获分离后激发吖啶衍生物发光,用化学发光分析仪检测光强度,计算标本中HBsAg的浓度.结果 该法线性方程为Y=0.919X +4.28,相关系数r2为0.99,线性范围为0.019 7~250.00 IU/mL.灵敏度可达0.020 7 IU/mL.0.1、3.2和40 IU/mL样本各重复测定10次的变异系数(CV)值分别为2.70%、4.40%和2.70%.与Abbott公司HBsAg定量检测试剂盒(化学发光微粒子免疫检测法)检测40份标本浓度的线性相关方程为Y=0.980 8X+0.209 9,r2=0.99,两法结果差异无统计学意义(江1.519,P>0.05).以10、50、100和200 IU/mL作为检定点,各检定点相对偏差分别为0.18%、1.50%、1.71%和1.81%.结论 建立的方法有较高的灵敏度、合适的线性,同时具备良好的可重复性,与Abbott公司试剂盒检测结果高度相关.

  11. Los complejos Chaperonina(MSP63inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica.

    Directory of Open Access Journals (Sweden)

    Juan Marzoa

    2009-08-01

    Full Text Available Alteration of the native structure of antigens can lead to the loss of protective epitopes. Our previous results showed that separation of the meningococcal outer membrane proteins in native conditions revealed the existence of protein complexes that could be relevant for the development of new vaccine formulations. The aim of this work was to analyse the immunogenic characteristics of a highly conserved 700 kDa chaperonin complex (CxChap detected and purified by using high resolution clear native electrophoresis. Analysis of the anti-CxChap serum by Western-blotting revealed the presence of antibodies against the MSP63 but also against the macrophage infectivity potentiator-like protein (MIP, which is coopurified with the chaperonin complex. Antibodies raised by immunisation with CxChap chaperonin complex show bactericidal and opsonophagocytic activity.

  12. 青少年HBsAg携带率12年前后的变化及影响因素分析%The change of HBsAg carrier rate of youngsters 12 years before and later and its influencing factors

    Institute of Scientific and Technical Information of China (English)

    余进胜; 陈建华; 林振南; 雷建国

    2009-01-01

    Objective To investigate the change of HBsAg carrier rate of youngsters 12 years before and later in Huidong region. Methods HBsAg census was carried out by mean of physical examination for youngsters; HBsAg was tested by using ELISA. Results (1) The HBsAg positive rates children in nursery school and junior high school admission students from 2005 to 2008 were decreased year by year; (2) The HBsAg positive rates of high school admission students in 2008 were significantly higher than that of junior high school admission ones in 2008; (3) Compared with that of 12years ago, the HBsAg positive rate of high school admission students was decreased significantly; (4)As the same crowd, their HBsAg positive rate at physical examination before the college entrance examination was higher than that of high school admission. Conclusion (1) Immune interruption of the transmission from mother to baby and expanded program on immunization (EPI) control hepatitis B infection effectively; (2) High school girls have a higher HBsAg positive rate at physical examination before college entrance examination than that when they enter the high school, which may be a result of learning tension in high school. A school girl may be infected hepatitis B by the route of horizontal transmission, which is associated with their physiological factors.%目的 调查惠东地区青少年HBsAg携带率,分析受调查群体12年前后HBsAg携带率的变化.方法 对青少年以健康体检的形式进行HBsAg普查;HBsAg采用ELISA法检测.结果 (1)2005-2008年入托儿童、初中入学生的HBsAg阳性率逐年下降;(2)2008年高中入学组HBsAg阳性率明显高于初中入学组;(3)高中入学人群与12年前比较HBsAg阳性率明显下降;(4)同一人群高考体检比高中入学时的HBsAg阳性率高.结论 (1)母婴免疫阻断和新生儿计划免疫有效地控制了乙型肝炎的感染;(2)高中女生的HBsAg阳性率高考前比高中入学时高,可能是高中阶段学习紧

  13. Influence factors of quantitative changes of dendritic cells in neonate born to HBsAg positive mother%HBsAg阳性母亲新生儿树突状细胞数量变化的影响因素

    Institute of Scientific and Technical Information of China (English)

    郭健; 高怡; 郭珍; 付振东; 郝海燕; 汪波; 王素萍

    2012-01-01

    Objective To investigate the influence factors of quantitative changes of dendritic cells (DC) in neonate born to HBsAg positive mother.Methods Sixty HBsAg positive mothers and their newborns were enrolled from the Third People's Hospital of Taiyuan from July 2011 to March 2012.The serum hepatitis B virus (HBV) markers and HBV DNA in mothers and newborns before vaccination were determined by chemiluminescence immunoassay (CLIA) and fluorescence quantitative polymerase chain reaction (PCR).The circulating frequencies of DC subsets were determined in the newborns by flow cytometry (FCM).The comparison of data was done by Mann-Whitney test and t test.The correlation analysis was done by Spearman rank correlation analysis and chi square test.Results Among 60 newborns,5 were HBsAg positive and HBV DNA negative.Among 60 HBsAg positive mothers,21 were HBeAg positive and 29 were HBV DNA positive.There was no significant quantitative difference of neonatal myeloid dendritic cells (mDC) and plasmacytoid dendritic cells (pDC) between intrauterine infection group and intrauterine non-infection group (Z=-0.535,P=0.59 and Z=-0.027,P=0.98,respectively).However,mother's HBeAg positive status was closely related with neonatal HBeAg positive status (Pearson contingency coefficient was 0.928,P<0.01).The frequencies of mDC in newborns born to HBeAg positive mothers were significantly lower than those born to HBeAg negative mothers (0.60±0.57 vs 0.87±0.58; Z=-2.085,P<0.05).However,there was no significant quantitative differences of mDC and pDC between newborns born to HBV DNA positive mothers and born to negative mothers (Z=-1.272,P=0.20 and Z=-0.806,P=0.42,respectively).The frequencies of pDC were significantly lower in newborns born to mothers with HBV DNA> 1 × 107 copy/mL compared to newborns born to HBV DNA negative mothers (0.30±0.18 vs 0.64±0.55; t=-2.996,P=0.005).Conclusions HBeAg positive status of mothers may reduce neonatal frequencies of mDC.Neonatal frequencies

  14. HBsAg阴性献血者输血HBV感染残余风险分析%Residual Risk of Transfusion-transmitted HBV Infection in HBsAg-negative Blood Donors

    Institute of Scientific and Technical Information of China (English)

    方昌志; 傅颖媛; 钱榕; 熊丽红

    2012-01-01

    目的 分析HBsAg ELISA法检测阴性献血者的输血HBV感染残余风险,评价其感染状况.方法 采用瑞士罗氏诊断公司的Cobas s201核酸检测平台对2011年1月1日至12月31日57 141人份2遍ELISA检测阴性标本进行HBV DNA/HCV RNA/HIV-1,-2 RNA三项联合核酸检测(Cobas TaqScreen MPX 试剂),检测模式为混样检测+拆分检测,即先进行6标本混样检测,再对检测阳性标本进行拆分检测;对127人份拆分检测阳性标本进行分项鉴别试验及乙型肝炎两对半检测.结果 1)2遍ELISA法共检测标本60 037人份,检出HBsAg阴性标本57 141人份;2)对57 141人份阴性标本进行HBV DNA/HCV RNA/HIV-1,-2 RNA三项联检,共检出127人份病毒核酸阳性标本;3)127人份病毒核酸阳性标本进行分项鉴别试验,共检出HBV DNA 阳性标本69人份,输血HBV残余风险为0.12%,其定量检测结果以<20 U·mL-1为主(51人份,占73.9%);4)69人份HBV DNA阳性标本进行乙型肝炎两对半检测,发现乙型肝炎可疑窗口期感染6人份(占8.7%),隐匿性感染 52人份(占75.4%).结论 HBsAg ELISA法检测阴性献血者的输血HBV感染残余风险依然存在,其感染状况多以隐匿性感染为主,将核酸检测纳入血液筛查常规模式,可降低输血残余风险,提高输血安全.%Objective To analyze the residual risk of transfusion-transmitted HBV infection by ELISA method in HBsAg-negative blood donors,and to assess the infection status. Methods TaqScreen MPX test was performed for the detection of HBV DNA/HCV RNA/HIV-1,-2 RNA on a Cobas s201 system (Swiss Roche Diagnosis Company) in 57 141 HBsAg-negative blood donations. The detection was repeated two times. The detection mode was a combination of 6-sample minipool and individual positive donation test. Then, subentry identification and HBV marker detection were conducted in the 127 positive individual donations. Results A total of 60 037 samples were detected by twice ELISA test and 57 141 of them were

  15. Efetividade das vacinas anti-VHB (DNA-recombinante) em doadores de sangue de uma região endêmica para hepatite B no sul do Brasil Effectiveness of recombinant DNA vaccines against hepatitis B in blood donors in an endemic region of South Brazil

    OpenAIRE

    Andrea Petry; Emil J. Kupek

    2006-01-01

    O objetivo deste estudo foi de estimar a efetividade das vacinas anti-VHB em um estudo longitudinal, retrospectivo composto por 1.012 doadores de sangue que completaram o esquema padrão de vacinação (três doses, incluindo doses de reforço nos doadores com títulos de anti-HBs

  16. MicroRNA-122 as a predictor of HBsAg seroclearance in hepatitis B and C dual infected patients treated with interferon and ribavirin

    Science.gov (United States)

    Yen, Yi-Hao; Huang, Chao-Min; Wei, Kuo-Liang; Wang, Jing-Houng; Lu, Sheng-Nan; Lee, Chuan-Mo; Hung, Chao-Hung; Chen, Chien-Hung; Tseng, Po-Lin; Chang, Kuo-Chin; Tsai, Ming-Chao; Lin, Ming-Tsung; Wu, Cheng-Kun; Yang, Cheng-Hong; Moi, Sin-Hua; Cho, Chung-Lung; Hu, Tsung-Hui

    2016-01-01

    It has been demonstrated that microRNA-122 (miR-122) plays key roles in the modulation of hepatitis B virus (HBV) replication. This study examined the role of miR-122 in patients with hepatitis C virus (HCV)-HBV dual infection with active hepatitis C who received pegylated interferon-α and ribavirin dual therapy. We enrolled 121 patients with HCV-HBV dual infection after dual therapy. Stored serum was collected before treatment. RT-PCR was used to analyze miR-122. HBsAg seroclearance was noted in 37 (30.1%) cases during a median follow-up period of 5.4 years. miR-122 was significantly lower in HBsAg seroclearance patients than in non-HBsAg seroclearance patients (P  100 IU/mL versus ≤100 IU/mL (P < 0.001). We concluded that in patients with HBV-HCV dual infection with active hepatitis C, miR-122 was associated with HBsAg seroclearance after therapy and qHBsAg level before therapy, indicating that miR-122 plays key roles in modulating HBV replication. PMID:27665934

  17. Anti-hepatitis B core antigen testing with detection and characterization of occult hepatitis B virus by an in-house nucleic acid testing among blood donors in Behrampur, Ganjam, Orissa in southeastern India: implications for transfusion

    Directory of Open Access Journals (Sweden)

    Panigrahi Rajesh

    2010-08-01

    Full Text Available Abstract Background Occult hepatitis B virus (HBV infection might transmit viremic units into the public blood supply if only hepatitis B surface antigen (HBsAg testing is used for donor screening. Our aim was to evaluate the prevalence of occult HBV infection among the HBsAg negative/antiHBc positive donations from a highly HIV prevalent region of India. Methods A total of 729 HBsAg negative donor units were included in this study. Surface gene and precore region were amplified by in house nucleic acid test (NAT for detection of occult HBV infection and surface gene was analyzed after direct sequencing. Results A total of 220 (30.1% HBsAg negative donors were antiHBc positive, of them 66 (30% were HBV DNA positive by NAT. HBV DNA positivity among 164 antiHBc only group, was 27.1% and among 40 antiHBs positive group was 30.0%. HBV/D (93.3% was predominant and prevalence of both HBV/C and HBV/A was 3.3%. Single or multiple amino acids substitutions were found in 95% samples. Conclusion Thus, a considerable number of HBV infected donors remain undiagnosed, if only HBsAg is used for screening. Addition of antiHBc testing for donor screening, although will lead to rejection of a large number of donor units, will definitely eliminate HBV infected donations and help in reducing HBV transmission with its potential consequences, especially among the immunocompromised population. The HBV genetic diversity found in this donor population are in accordance with other parts of India.

  18. Seroprevalencia de anticuerpos bactericidas frente al meningococo C en Cantabria 10 meses tras la campaña de vacunación

    Directory of Open Access Journals (Sweden)

    Aledo Linos Alvaro Gonzalez de

    1998-01-01

    Full Text Available FUNDAMENTO: En febrero-marzo de 1997 se realizó en Cantabria (Comunidad Autónoma del Estado Español con 541.885 habitantes, de los cuales 107.787 tienen una edad entre 18 meses y 19 años una campaña de vacunación antimeningocócica dirigida a niños entre 18 meses y 19 años, que abarcó a todos sus municipios y alcanzó una cobertura mayor del 95%. En los 12 meses posteriores la eficacia vacunal fue del 95.68% en todas las edades. Para ayudar a decidir una revacunación se realizó un estudio de seroprevalencia de anticuerpos bactericidas en la población vacunada. MÉTODOS: en diciembre de 1997 se analizaron muestras de sangre de 414 niños vacunados, obtenidas al azar de extracciones oportunistas realizadas en Atención Primaria y en Hospitales Públicos de la Comunidad, y de niños escolarizados en las guarderías públicas dependientes de la Dirección General de Bienestar Social de Cantabria. Se analizó la tasa de anticuerpos bactericidas en el Centro Nacional de Microbiología, estableciéndose el nivel de "efecto vacunal" en la dilución 1/8. RESULTADOS: se obtuvieron los siguientes porcentajes de títulos > o = 1/8 (entre paréntesis, grupos de edad al vacunarse: 0% (18-24 meses, 4% (1.5 a 4 años, 7.1% (1.5 a 6 años, 51.3% (6 a 12 años y 81.2% (12 a 19 años. Debido a que la definición de "efecto vacunal" se cifró en la dilución 1/8 de forma artificial, y a que otros trabajos la cifran en 1/4, en 287 sueros con resultado <1/8 se repitió el ensayo bactericida con la dilución 1/4, resultando que 286 (el 99.6% también dieron negativo. Es decir, que el resultado final no variaría si situásemos el punto de corte en la dilución 1/4 en vez de en 1/8. No se encontraron diferencias significativas según la procedencia de municipios donde hubiera habido casos de meningitis C o no los hubiera habido. CONCLUSIONES: la actividad bactericida es muy baja en los menores de 4-6 años, inferior a lo publicado, y mayor por encima de esa

  19. Prevalencia de anticuerpos contra virus dengue en el cantón de Golfito (2005 y en el Distrito Central de Puntarenas (2005-2006, Costa Rica

    Directory of Open Access Journals (Sweden)

    Margarita Lee-Lui

    2008-09-01

    Full Text Available Justificación y objetivo: La primo infección con el virus dengue varía desde asintomática hasta cuadros muy severos, como el dengue hemorrágico o el síndrome de choque por dengue. El Distrito Primero de Puntarenas ha sido una de las poblaciones más afectadas desde 1993, cuando aparecieron los primeros brotes, hasta hoy, con una disminución de la incidencia en los últimos años. La región Brunca fue la segunda en incidencia en el país en 1998, y si bien la endemicidad se ha mantenido, no existen estudios epidemiológicos sobre la prevalencia de esta enfermedad; los datos que aporta el Ministerio de Salud corresponden a la incidencia de casos clínicos en un período determinado. El objetivo de este estudio fue determinar la seroprevalencia en el Distrito Primero de Puntarenas y en Golfito para conocer la vulnerabilidad de la población de sufrir fiebre hemorrágica por dengue y analizar la situación asociada a los datos epidemiológicos de estas regiones, tales como edad, sexo y ubicación geográfica, ya que una población susceptible es la que presenta una alta seroprevalencia, unida a condiciones de hacinamiento y alta densidad vectorial. Métodos: El muestreo se realizó con el método EPI descrito por la OMS. Se recolectaron 210 muestras de suero en cada distrito, se desarrolló y estandarizó una prueba de ELISA de captura de antígeno para la detección de anticuerpos tipo IgG contra dengue, usando como referencia el método de reducción de placas de Dulbecco considerado estándar de oro. Resultados: La seroprevalencia contra dengue fue del 43.8% (92 y del 90% (189 en los distritos de Golfito y Central de Puntarenas, respectivamente, sin diferencias significativas respecto al sexo, la edad y la localidad. Se obtuvo un 83% y un 95% de correlación en Golfito y Puntarenas, respectivamente, entre los casos sintomáticos y la serología con el método estandarizado. Un 32% y un 42% del total de seropositivos en cada localidad

  20. SISTEMA INMUNE Y GENÉTICA:: UN ABORDAJE DIFERENTE A LA DIVERSIDAD DE ANTICUERPOS Immune System and Genetics:: A Different Approach to the Diversity of Antibodies

    Directory of Open Access Journals (Sweden)

    NUBIA ESTELA MATTA CAMACHO

    Full Text Available Es común encontrar en los libros de inmunología o de genética un capítulo con el título de -sistema inmune y genética-, sin embargo su asociación se centra en cómo la generación de anticuerpos rompió el paradigma -un gen, una proteína-, pues en el caso de la producción de anticuerpos, un gen produce millones de proteínas. El sistema inmune tiene muchos vínculos con la genética y la herencia; esta asociación se da porque cualquier sustancia o compuesto que produzca un organismo, es un antígeno potencial cuando es reconocido como extraño por el sistema inmune de otro organismo, sea este de la misma o de diferente especie. La producción de proteínas que potencialmente pueden ser antigénicas están ligadas al genotipo del individuo. La capacidad de responder y el tipo e intensidad de respuesta a antígenos también ha sido demostrado que están correlacionados con el genotipo del individuo en cuestión, así como deficiencias en las respuestas inmunes pueden estar asociadas con mutaciones o polimorfismos genéticos que resultan en la susceptibilidad a enfermedades infecciosas. Este manuscrito busca presentar ejemplos de la relación sistema inmune - genética, en la misma dirección de la conferencia ofrecida para la cátedra de Sede -Todo lo que usted quiere saber de genética y nunca se atrevió a preguntar-.It is common to find in immunology or genetic books a chapter entitled -immune system and genetics-; this association focuses on how the generation of antibodies broke the paradigm -one gene, one protein-, since in this case one gene generates millions of proteins. However, the immune system has many more links to genetics and heredity. For example, any substance or compound produced by an organism can be a potential antigen, when it is recognized as foreign by the immune system of another organism, belonging to the same or different species. The proteins, which are potentially antigenic are encoded by the individual s

  1. Faster heme loss from hemoglobin E than HbS, in acidic pH: Effect of aminophospholipids

    Indian Academy of Sciences (India)

    Mousumi Banerjee; Malini Pramanik; Dipankar Bhattacharya; Mohini Lahiry; Samita Basu; Abhjit Chakrabarti

    2011-12-01

    We report studies on loss of heme at or below pH 3.0 from two clinically important hemoglobin variants, HbE and HbS, in the presence and absence of phopholipid membranes. The kinetics of heme loss has been studied at pH 3.0 to simulate the same at a faster rate than at physiological pH, for spectroscopic investigation. Results obtained from the study clearly establish the probable fate of the lost heme to partition into the phospholipid bilayer independent of the pH range. This is also of particular importance to membranes containing the aminophospholipid and cholesterol which are predominantly localized in the inner leaflet of erythrocytes. Absorption measurements indicated such loss of heme when the Soret peak at 415 nm blue-shifted to 380 nm at pH 3.0. The extent of this blue shift decreased from 35 nm to ∼15 nm in the presence of small unilammelar vesicles of both dimyristoyl- and dioleoyl-based phosphatidylcholine and phosphatidylethanolamine, indicating partitioning of the released heme in the membrane bilayer. The kinetics of heme loss was faster from HbE than HbA and HbS, obeying first-order reaction kinetics. Released heme could be involved in the premature destruction of erythrocytes in hemoglobin disorders.

  2. Nivel de corte de los ELISAs para cuantificación de anticuerpos inducidos por la vacuna antimeningocócica VA-MENGOC-BC

    Directory of Open Access Journals (Sweden)

    Rolando Ochoa

    2001-06-01

    Full Text Available Para medir el grado de protección inducido por vacunas antimeningocócicas se ha establecido el Ensayo Bactericida en Suero (EBS y se perfeccionan otros ensayos inmunobiológicos, sin embargo, es necesario contar con pruebas sencillas como el ELISA, capaz de evaluar un gran número de muestras. Se estimó el nivel de corte de los ELISAs para la cuantificación de IgG humana contra los antígenos de VA-MENGOC-BC, vacuna antimeningocócica compuesta por vesículas proteicas de membrana externa de meningococo B y polisacárido capsular de meningococo C, con respecto a un panel de muestras de suero de lactantes, caracterizado por Ensayo Bactericida en Sangre Total (EBST. Los valores correspondientes a la máxima sensibilidad y especificidad fueron respectivamente; 2 μg/mL y 12 μg/mL para antipolisacárido C, y 1000 U/mL y 7000 U/mL para antiproteínas de membrana externa. La mayor coincidencia se obtuvo con 6 μg/mL y 2500 U/mL. Se evaluó otro panel de muestras de suero de adolescentes entre 14 y 18 años, por ELISA y EBS para Neisseria meningitidis serogrupos B y C, alcanzándose una buena concordancia. Doce años después de la inmunización con VA-MENGOC-BC persiste una importante concentración de anticuerpos contra los antígenos vacunales en los sueros estudiados.

  3. Detección de anticuerpos contra Trypanosoma cruzi utilizando discos de 3 mm en muestras de sangre seca sobre papel de filtro

    Directory of Open Access Journals (Sweden)

    Idialis Hernández-Spengler

    2014-01-01

    Full Text Available La utilización de sangre seca sobre papel de filtro ha despertado gran interés en los últimos años en los inmunoensayos debido a las ventajas que ofrece este modo de presentación de las muestras, lo que permite su utilización en países con recursos limitados para realizar estudios epidemiológicos. En este trabajo, se determinaron las condiciones óptimas de elución de muestras de sangre seca sobre papel de filtro para discos de 3 mm, como referencia se tomaron muestras de suero y las condiciones de elución establecidas para discos de 5 mm, en la detección de anticuerpos específicos a Trypanosoma cr uzi mediante el ensayo inmunoenzimático indirecto UMELISA ® CHAGAS ( Tecnosuma Internacional S.A. basado en el Sistema Ultra Micro Analítico (SUMA ® . Se evaluaron 12 muestras de suero positivos a la enfermedad de Chagas procedentes de Colombia confirmados p or inmunofluorescencia indirecta (IFI y ELISA – Chagatek ® y 75 muestras de sangre seca sobre papel de filtro procedentes de Bolivia reactivas por el UMELISA ® CHAGAS. Además, se evaluaron muestras de suero y sangre seca sobre papel de filtro negativas a la enfermedad de Chagas procedente de donantes sanos del banco de sangre provincial de La Habana. Los resultados del estudio de sensibilidad y especificidad para discos de 3 mm y 5 mm fueron similares. La reducción del diámetro del disco permite, a partir d e una misma mancha, realizar una mayor cantidad pruebas de diagnósticos para la detección de otras enfermedades, como el síndrome de inmunodeficiencia humana adquirida, hepatitis B y C entre otras.

  4. Prueba de Elisa indirecta para la detección de anticuerpos IgM para el diagnóstico de Leptospirosis humana

    Directory of Open Access Journals (Sweden)

    Manuel Céspedes Z

    2002-01-01

    Full Text Available Para el diagnóstico temprano de enfermedades con cuadro clínico inespecífico como la leptospirosis, es necesario la confirmación laboratorial mediante pruebas específicas, con la finalidad de que el diagnóstico sea más acertado y rápido. Objetivo: se realizó un estudio comparativo entre la prueba de microaglutinacion (MAT y la prueba de ELISA indirecta estandarizada con un "pool" de antígenos de Leptospira interrogans, para la detección de anticuerpos IgM, en muestras de suero de fase aguda de leptospirosis humana. Materiales y métodos: 40 muestras de pacientes con sospecha clínica y con títulos de 1:100-1:12800 por la prueba de MAT, 80 muestras negativas de pacientes aparentemente sanos con enfermedades como Brucelosis, Sífilis, Tifus murino, Hepatitis B, Fiebre Amarilla, Dengue y Enfermedad de Carrión fueron evaluados por ELISA IgM. Resultados: se obtuvo una sensibilidad de 97,5% y especificidad de 98,75%, no observándose reacción cruzada con otras enfermedades. Conclusión: ELISA IgM validado en el laboratorio es suficiente sensible, específico y de fácil aplicación para el uso como prueba de tamizaje en una infección por Leptospiras con la subsecuente confirmación por MAT.

  5. 献血人群血清anti-HBC和HBV DNA检测的意义

    Institute of Scientific and Technical Information of China (English)

    朱紫苗; 谢步飞

    2014-01-01

    目的 探讨无偿献血者乙型肝炎病毒核心抗体与隐匿性感染的关系.方法 收集无偿献血者样本9 100份,采用ELISA法进行HBsAg和anti-HBC血清学筛查,对anti-HBC阳性血清PCR检测乙肝病毒核酸.结果 911份(10.01%)标本为anti-HBC阳性,其中820份(90.01%)HBsAg阴性,34例血清HBV DNA阳性.结论 常规检测对于献血者筛查具有重要意义.

  6. Generación de anticuerpos policlonales para la detección de la variante genotípica GIII de PVY, en cultivos de tomate de árbol y papa de Colombia

    Directory of Open Access Journals (Sweden)

    Yuliana Gallo García

    2012-04-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Título en ingles: Generation of policlonal antibodies for detection of the PVY genotypic variant GIII in tamarillo and potato crops from Colombia Resumen: El potyvirus PVY es uno de los agentes causales más frecuentemente asociados a problemas virales en cultivos de papa y tomate de árbol en Colombia. Dada la importancia económica de las enfermedades causadas por PVY y a la necesidad de generar material de siembra certificado por su sanidad viral, es fundamental la generación de herramientas de diagnóstico que permitan la detección temprana de este virus. En este trabajo se reporta la obtención de anticuerpos policlonales específicos, útiles para la detección del genotipo III de PVY (GIII, una de las tres variantes que recientemente han sido reportadas en cultivos de papa y tomate de árbol de la región Andina de Colombia. Como antígeno, se utilizó un péptido sintético diseñado a partir de la región variable del extremo N-terminal del gen de la cápside viral. La sensibilidad de los anticuerpos fue evaluada mediante pruebas de ELISA y dot-blot utilizando péptidos sintéticos. Se realizó una prueba piloto para validar el uso de los anticuerpos a partir de plantas sintomáticas y asintomáticas obtenidas de una región donde confluyen cultivos de ambas solanáceas, encontrándose que los anticuerpos generados ofrecen mayores niveles de detección que los anticuerpos comerciales comúnmente utilizados para detectar los serotipos PVY-O,C y PVY-N de este virus. Palabras clave: anticuerpos policlonales; ELISA; potyvirus; RT-PCR; virus Y de la papa Abstract: PVY is one of the potyvirus more frequently associated with viral infections in tomato and tamarillo crops in Colombia. Due to the economic impact of PVY and the need to certify seeds as virus-free it is important to develop diagnostic tools that allow its premature detection. In this work, the

  7. Serum soluble interleukin-2 receptor levels in patients with chronic hepatitis B virus infection and its relation with anti-HBc

    Institute of Scientific and Technical Information of China (English)

    Ping Xiao; Qing-Feng Chen; Yan-Ling Yang; Zhen-Hua Guo; Hong Chen

    2006-01-01

    AIM: To investigate the relationship between serum soluble interleukin-2 receptor (sIL-2R) level and anti-HBc in patients with chronic hepatitis B virus (HBV) infection.METHODS: Sera from 100 patients with chronic HBV infection and 30 healthy controls were included in this study. The patients were divided into group A [HBsAg (+),HBeAg (+) and anti-HBc (+),n = 50] and group B [HBsAg(+), HBeAg (+) and anti-HBc (-), n = 50]. sIL-2R levels were determined using ELISA. HBV DNA and alanine aminotransferase (ALT) were also detected.RESULTS: Serum sIL-2R levels were significantly higher in patients with chronic HBV infection than in healthy controls. Moreover, serum sIL-2R levels were significantly higher in patients with HBsAg (+), HBeAg (+) and antiHBc (+) (976.56±213.51×L03 U/L) than in patients with HBsAg (+), HBeAg (+) and anti-HBc (-) (393.41 ± 189.54×103 U/L, P<0.01). A significant relationship was found between serum sIL-2R and ALT levels (P< 0.01) in patients with chronic HBV infection, but there was no correlation between sIL-2R and HBV DNA levels. The anti-HBc status was significantly related to the age of patients (P< 0.0t).CONCLUSION: The high sIL-2R level is related to positive anti-HBc in chronic hepatitis B patients. Positive anti-HBc may be related to T-lymphocyte activation and negative anti-HBc may imply immune tolerance in these patients.

  8. Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L

    OpenAIRE

    Long Ma; Haibo Li; Jun Zhao; Tao Liu

    2010-01-01

    Suosuo grape (Vitis vinifer L) is traditionally used as a therapeutic agent for measles and hepatitis by the ethnic Uighurs. This work aimed to investigate the anti-HBV effect of total triterpene (VTT), total flavonoids (VTF) and total polysaccharides (VTP) from Suosuo grape, and their synergistic effects were also tested. The viral antigens of cellular secretion, HBsAg and HBeAg, were determined by enzyme linked immunosorbent assay (ELISA).The quantity of HBV-DNA released in the supernatant ...

  9. Analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-HBsAg for pharmaceutical use

    OpenAIRE

    Valdes, Rodolfo; Medina, Yenisley; Fernández, Eutimio G.; Geada, Déborah; Ferro, Williams; Álvarez, Tatiana; Padilla, Sigifredo; Guevara, Yaryse; Tamayo, Andrés de; Gomez, Leonardo; Montero, José; Figueroa, Alejandro; Calas, Gisela; García A., Cristina

    2008-01-01

    Immunoaffinity chromatography (IAC) has a wide application in protein purification. The aim of the study was to investigate the influence of five operational factors on an IAC procedure to purify a yeast derived-Hepatitis B surface antigen (rHBsAg) for pharmaceutical use. The immunosorbent adsorption capacity of the rHBsAg is affected at 4 °C. The applied antigen concentration, 100-1000 μg mL-1, does not have influence on this IAC efficiency. The residence time, applied antigen amo...

  10. Operating Experience of Collection of Femoral Vein Blood in Neonates of HBsAg-positive Puerpera%HBsAg阳性产妇所产新生儿股静脉取血的操作体会

    Institute of Scientific and Technical Information of China (English)

    尤炜; 轧春妹; 张平; 刘学兰; 陈颖杰

    2011-01-01

    目的 探讨HBsAg阳性产妇所产新生儿股静脉取血的适宜时间,以期为预防新生儿感染提供依据.方法 1999年10月至2010年1月,天津市传染病医院住院及门诊HBsAg阳性产妇所产足月新生儿21 348例,按随机数字表法将21 348例新生儿分为观察组(10 826例)与对照组(10 522例).观察组新生儿于出生后2 h至4周经股静脉取血检测乙型肝炎5项和HBVDNA定量,对照组新生儿于出生后4~52周经股静脉取血检测相同项目,比较两组新生儿一次针刺取血的成功率.结果 观察组新生儿一次针刺取血成功率明显高于与对照组新生儿,差异有统计学意义(χ2=233.868,P<0.01).结论 新生儿出生4周内静脉取血检测乙型肝炎五项和HBVDNA定量更容易操作,且可以尽早地为临床提供新生儿HBV感染的相关信息,对乙型肝炎阻断治疗的实施意义重大.%Objective To explore the collection time of femoral vein blood in neonates of HBsAg-positive puerpera so as to provide the evidence for protecting infections of neonates. Methods From October 1999to January 2010,21348 full-term neonates were delivered by HBsAg-positive mother in our inpatient and outpatient department. The 21348 neonates were randomized into observation group(n= 10 826) and control group(n=10 522). The neonates in the observation group were collected of femoral vein blood to test hepatitis B makers and HBV-DNA between 2 h and 4 w after birth. While the neonates in the control.group were collected of femoral vein blood for the same test between 4 w and 52 w after birth. Comparison was conducted on the one-time success rate of acupuncture for blood collection. Results The one-time success rate for taking blood of the newborns of the observation group was significantly higher than that of the control group (x2 = 233..868, P<0.01). Conclusion Collection of femoral vein blood of the newborns within 4w after birth is more convenient for five tests of Hepatitis B and

  11. Comparison of Test Results of HBsAg in Serum and Plasma by Automatic ELISA Analysis%血清与血浆中HBsAg在全自动酶免分析检测结果比较

    Institute of Scientific and Technical Information of China (English)

    李小溪; 李筱涵; 郭晓东; 李波; 孙志强

    2011-01-01

    目的:探讨血浆取代血清检测乙肝标志物的检测结果以及临床意义.方法:选取我科检测乙肝标志物的血样30份,分别放在抗凝剂管和普通干燥试管,采用全自动酶免疫分析仪检测HBsAg,将检测结果进行OD及S/CO值统计处理,并进行比较分析.结果:血清与二种血浆检测结果相关系数均0.99,二种血浆与血清结果相关关系良好(P>0.0 5),无显著性差异.结论:血浆代替血清完全可以用于全自动酶标分析仪进行检测,既可以节省预处理时间,又可以减少标本在分离吸移血清过程中出现差错,值得临床推广和应用.%Objective: To investigate the clinical significance of the plasma instead of the serum in the detection ofHBV markers.Methods: A total of 30 blood samples were selected and detected HbsAg by automatic enzyme immunoassay analyzer, The test results were statistically treated and comparatively analyzed. Results: Serum and plasma results of two correlation coefficients were 0.99, two kinds of relationship between plasma and serum results well (P>0.05), no significant difference. Conclusion: Plasma instead of serum enzyme fully automated analyzer can be used to detect both pre-save time and reduce the serum specimens in the process of separation pipette errors and is worthy of promotion and application.

  12. Hepatitis B virus surface antigen and anti-hepatitis C virus rapid tests underestimate hepatitis prevalence among HIV-infected patients

    DEFF Research Database (Denmark)

    Hønge, Bo Langhoff; Jespersen, S; Medina, C;

    2014-01-01

    OBJECTIVES: In the case of coinfection with HIV and hepatitis B virus (HBV) and/or hepatitis C virus (HCV), hepatic disease progression is often accelerated, with higher rates of liver cirrhosis and liver-related mortality. We aimed to evaluate the performance of the rapid tests used routinely...... to detect HBV surface antigen (HBsAg) and anti-HCV among HIV-infected patients in Guinea-Bissau. METHODS: Blood samples from HIV-infected patients in Guinea-Bissau were stored after testing for HBsAg and anti-HCV with rapid tests. Samples were subsequently re-tested for HBsAg and anti-HCV in Denmark....... RESULTS: Two rapid tests were used in Guinea-Bissau: HBsAg Strip Ref 2034 (VEDA.LAB, Alençon, France; sensitivity 62.3%; specificity 99.2%) and HEPA-SCAN (Bhat Bio-Tech, Bangalore, India; sensitivity 57.1%; specificity 99.7%). In the two tests the ability to obtain the correct outcome depended...

  13. 钙网蛋白融合HBsAg基因重组腺病毒新型载体疫苗的构建与鉴定%Construction and characterization of a novel therapeutic vaccine of recombinant adenovirus vector containing calreticulin/HBsAg fusion gene

    Institute of Scientific and Technical Information of China (English)

    张兰春; 王宝红; 王芳; 马春玲

    2011-01-01

    Objective:To generate recombinant adenoviral vector containing CRT-HBsAg fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine.Methods:The fusion of CRT and HBsAg gene was constructed by using polymerase chain reaction(PCR), endonuclease digestion and ligation methods, and then the fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5' end.Adenoviral expression vector(Ad-CRT/HBsAg)containing CRT-HBsAg fusion gene was constructed by homologous recombinantion.The linearized DNA plasmid of the recombinant adenoviral vector was transfected into human embryo kidney (HEK 293A) cells to package and amplify recombinant adenovirns.The recombinant adenovirus titer was characterized by using the End-dilution assay.The expression of the CRT/HBsAg fusion protein in Ad-CRT/HBsAg transfected 293A cells was detected by Western blot.Results:The CRT-HBsAg fusion gene was characterized by using PCR, and sequencing result revealed that the length and sequence were accurate.The recombinant adenoviral vector, Ad-CRT/HBsAg, was generated successfully.The titer of Ad-CRT/HBsAg was characterized as 2.68×1011 pfu/ml.The CRT-HBsAg fusion protein was expressed by HEK 293A cells correctly.Conclusion:Recombinant replication-defective adenovirus expression vector containing CRT/HBsAg fusion gene was constructed successfully, and this study has provided an experimental basis for further research of HBV gene therapy.%目的:构建表达钙网蛋白(calreticulin,CRT)与乙型肝炎病毒表面抗原(hepatitis B surface antigen,HBsAg)融合基因重组腺病毒载体(Ad-CRT/HBsAg),为研发新型乙型肝炎病毒(hepatitis B virus,HBV)治疗性疫苗奠定基础.方法:采用腺病毒表达系统(ViraPowerTM Adenofiral Expression System)构建重组腺病毒表达载体.首先利用RT-PCR的方法扩增CRT基因,并进一步构建CRT与HBsAg基因融合重组的pJW4303表达载体,在构建过程中给

  14. Blood Staions’ Study on the Correlation between HBsAg detection via ELISA and the Reactive Samples with PCR%血站HBsAg反应性样本的ELISA与PCR法检测结果的相关性

    Institute of Scientific and Technical Information of China (English)

    冯娟

    2016-01-01

    Objective To explore the agreement between the testing results of reactive samples by ELISA for HBsAg and HBV DNA by PCR,so as that a more accurate testing may be provided for the donors.Methods PCR was apply to the reactive samples from volunteer donors who had been tested by ELISA for HBsAg to make a comparison between the results. Results By employing two ELISA reagents,698 samples are tested to be reacted positively in the total of122,675. Among the698 samples, there are382 have been equally detected positive by the two ELISA reagents.203 cases were found PCR positive, account for29.08%.179 cases are PCR negative, account for25.64%. Among the204 positive samples tested by one ELISA reagent,8 cases are found PCR positive, account for1.15%;196 negative cases existed, account for28.08%. By employing two ELISA reagents,5 samples were within the gray area. None of them were positive for PCR, account for0%; while5 negative were observed, account for0.72%; by employing one single ELISA reagent,107 cases were within the gray area and5 cases were PCR positive, comprise0.72%;102 are negative, account for14.61 %.Conclusion In the HBsAg blood screening, different ELISIA reagents showed different degrees of sensitivity and specificity. If double reagents are applied, the positive reaction results are more likely to be consistent with the results from HBV DNA. A few cases within gray area are also identical with them, but the development of HBV DNA should be closely observed and followed-up.%目的:探讨无偿献血者ELISA法检测HBsAg反应性样本与PCR检测HBV DNA结果的符合程度,为献血者提供更为准确的检测结果;并探讨新模式下,两种检测方法的结果报告及血源的召回制度。方法对无偿献血者ELISA法检测HBsAg反应性样本,采用PCR检测,并进行结果比较。结果122657份献血者样本中,2种ELISA试剂共检测出反应性样本698例,其中2种ELISA试剂检测均为阳性样本382

  15. Detection of PMTV Using Polyclonal Antibodies Raised Against a Capsid-Specific Peptide Antigen / Detección de PMTV Utilizando Anticuerpos Policlonales Contra un Péptido Antigénico Derivado de la Cápside Viral

    Directory of Open Access Journals (Sweden)

    Yuliana Gallo García

    2013-12-01

    Full Text Available Potato mop-top virus (PMTV; genus Pomovirus;family Virgaviridae is the causing agent of the spraing disease in potato (Solanum tuberosum. PMTV is transmitted by Spongospora subterranea f. sp. subterranea (Sss. This disease has a widespread distribution in potato growing regions around the world. The possibility of obtaining strain specific antibodies at low cost can greatly increase the sensitivity and use of serological tests in seed certification programs, plant breeding and quarantine regulations to avoid dissemination of this injurious virus. This work presents an alternative procedure for the production of PMTV specific antibodies useful in serological test such as ELISAand lateral flow. In contrast to standard methods requiring theisolation of viral particles or expression of recombinant capsid, this method uses peptides mimicking the N-terminal region of PMTV capsid protein as antigen for the production of specific polyclonal antibodies. The antibodies were tested against bait plants grown in soil infested with viruliferous Sss, as well as potato plants obtained from naturally Sss infested fields in Colombia. PMTV was detected in 9/14 and 24/28 foliage samples of N. benthamiana and S. phureja, respectively. In the case of field plants, the virus wasdetected in eight out of 12 root tissues evaluated. The minimumpeptide concentration detected by ELISA was of the order of 0.1 nM. / Potato mop-top virus (PMTV; género Pomovirus; familia Virgaviridae es transmitido por Spongospora subterranea f. sp. subterranea (Sss, agente causal de la sarna polvosa de la papa. Esta enfermedad tiene una amplia distribución en las regiones cultivadoras de papa alrededor del mundo. La posibilidad de obtener anticuerpos específicos contra cepas de este virus, puede incrementar la sensibilidad y la utilización de pruebas serológicas en programas de certificación de semilla, mejoramiento genético y regulaciones cuarentenarias que eviten su diseminaci

  16. Prevalencia de anticuerpos anti-Chlamydia trachomatis y anti-Neisseria gonorrhoeae en grupos de individuos de la población mexicana Prevalence of antibodies against Chlamydia trachomatis and Neisseria gonorrhoeae in Mexican populations

    OpenAIRE

    María del Carmen Cravioto; Oscar Matamoros; Yvonne Villalobos-Zapata; Oscar Peña; Enrique García-Lara; Maribel Martínez; Julio Castelo; José Sifuentes-Osornio

    2003-01-01

    OBJETIVO: Estimar la prevalencia de infección por Chlamydia trachomatis (CT) y Neisseria gonorrhoeae (NG) en grupos de individuos con diferente riesgo para infecciones de transmisión sexual. MATERIAL Y MÉTODOS: Estudio transversal multicéntrico efectuado en el Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, de la Ciudad de México, de enero de 1992 a diciembre de 1993, en el que se estudiaron 945 individuos en edad reproductiva, 585 mujeres y 360 hombres. Según su riesgo p...

  17. Optimización del ensayo vibriocida colorimétrico para la determinación de anticuerpos séricos contra V. cholerae del serogrupo O139

    Directory of Open Access Journals (Sweden)

    Joivier Vichi-Lozada

    2011-01-01

    Full Text Available El título de anticuerpos vibriocidas es reconocido actualmente como el mejor marcador de inmunidad protectora ante la infección con Vibrio cholerae. Su evaluación permite estudiar la efectividad de vacunas durante ensayos preclínicos y clínicos, a través de la determinación de la funcionalidad de anticuerpos séricos, capaces de lisar células en presencia de complemento exógeno. Se han desarrollado distintos ensayos bactericidas con puntos finales y métodos de determinación del título vibriocida diferentes. La presencia de cápsula en el serogrupo O139 de Vibrio cholerae constituye un impedimento para la lisis eficiente de las células bajo condiciones del ensayo vibriocida utilizado en cepas del serogrupo O1. Por tal razón, se hace necesario adaptar los ensayos bactericidas existentes para V. cholerae O1, con la finalidad de utilizarlos con el serogrupo O139. A partir de un ensayo vibriocida colorimétrico, desarrollado para el serogrupo O1, se optimizaron las condiciones necesarias para una eficiente lisis celular del serogrupo O139 por anticuerpos específicos y el complemento. Este método colorimétrico tiene la ventaja que mediante la adición de glucosa y un indicador de pH al medio de cultivo utilizado para el crecimiento de las células sobrevivientes, puede determinarse visualmente el título de forma clara e inequívoca. Los mejores resultados se obtuvieron después del crecimiento de la cepa diana en cuñas de agar LB, al usar una concentración inicial de vibrión diana de 106 UFC/mL, una concentración inicial del complemento de 100 %, disolución salina fisiológica como diluente y tiempo de reacción de vibriolisis de una hora. Estas condiciones de experimentación permitieron obtener resultados exactos y precisos para V. cholerae O139.

  18. Genetic variation at MECOM, TERT, JAK2 and HBS1L-MYB predisposes to myeloproliferative neoplasms

    Science.gov (United States)

    Tapper, William; Jones, Amy V.; Kralovics, Robert; Harutyunyan, Ashot S.; Zoi, Katerina; Leung, William; Godfrey, Anna L.; Guglielmelli, Paola; Callaway, Alison; Ward, Daniel; Aranaz, Paula; White, Helen E.; Waghorn, Katherine; Lin, Feng; Chase, Andrew; Joanna Baxter, E.; Maclean, Cathy; Nangalia, Jyoti; Chen, Edwin; Evans, Paul; Short, Michael; Jack, Andrew; Wallis, Louise; Oscier, David; Duncombe, Andrew S.; Schuh, Anna; Mead, Adam J.; Griffiths, Michael; Ewing, Joanne; Gale, Rosemary E.; Schnittger, Susanne; Haferlach, Torsten; Stegelmann, Frank; Döhner, Konstanze; Grallert, Harald; Strauch, Konstantin; Tanaka, Toshiko; Bandinelli, Stefania; Giannopoulos, Andreas; Pieri, Lisa; Mannarelli, Carmela; Gisslinger, Heinz; Barosi, Giovanni; Cazzola, Mario; Reiter, Andreas; Harrison, Claire; Campbell, Peter; Green, Anthony R.; Vannucchi, Alessandro; Cross, Nicholas C.P.

    2015-01-01

    Clonal proliferation in myeloproliferative neoplasms (MPN) is driven by somatic mutations in JAK2, CALR or MPL, but the contribution of inherited factors is poorly characterized. Using a three-stage genome-wide association study of 3,437 MPN cases and 10,083 controls, we identify two SNPs with genome-wide significance in JAK2V617F-negative MPN: rs12339666 (JAK2; meta-analysis P=1.27 × 10−10) and rs2201862 (MECOM; meta-analysis P=1.96 × 10−9). Two additional SNPs, rs2736100 (TERT) and rs9376092 (HBS1L/MYB), achieve genome-wide significance when including JAK2V617F-positive cases. rs9376092 has a stronger effect in JAK2V617F-negative cases with CALR and/or MPL mutations (Breslow–Day P=4.5 × 10−7), whereas in JAK2V617F-positive cases rs9376092 associates with essential thrombocythemia (ET) rather than polycythemia vera (allelic χ2 P=7.3 × 10−7). Reduced MYB expression, previously linked to development of an ET-like disease in model systems, associates with rs9376092 in normal myeloid cells. These findings demonstrate that multiple germline variants predispose to MPN and link constitutional differences in MYB expression to disease phenotype. PMID:25849990

  19. Generación de anticuerpos monoclonales contra el antígeno específico de próstata (psa)para la detección del psa y su purificación

    OpenAIRE

    Acevedo Castro, Boris Ernesto

    2012-01-01

    El cáncer de próstata en Cuba es un problema de salud (2 672 casos diagnosticados y 2 769 defunciones en el 2007). Varios métodos diagnósticos se han implementado para la detección y manejo de esta enfermedad, destacando entre ellos la determinación serológica del antígeno específico de próstata (PSA). En el presente trabajo se generó y caracterizó un panel de 11 anticuerpos monoclonales (AcMs) IgG1 que detectan con alta afinidad los epítopos principales descritos del PSA, tanto en solución c...

  20. Diagnóstico rápido de citomegalovirus (CMV en pacientes inmunocomprometidos mediante anticuerpos monoclonales que reconocen proteinas precoces virales Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by using monoclonal antibodies against early viral antigens

    Directory of Open Access Journals (Sweden)

    Maritza Alvarez

    1989-06-01

    Full Text Available Se aplicó la técnica de detección de antigenos precoces fluorescentes (DAPF usando el anticuerpo monoclonal E-13 McAb, mediante el cual se lograron detectar 15 casos positivos a CMV de 75 muestras de orina o sangre ("buffy coat" tomadas de 52 pacientes inmunocomprometidos ingresados en el Instituto de Nefrología de ciudad Habana. Aplicando las técnicas clásicas de aislamiento en fibroblastos humanos diploides (MRC-5, se lograron aislar 12 cepas de CMV de casos previamente positivos por DAPF; lográndose además un aislamiento en una muestra reportada negativa por fluorescencia. Se observó una coincidencia de un 80% entre ambas técnicas. Se detectó la presencia de anticuerpos IgG contra CMV en todos los casos estudiados, utilizando para ello la técnica ELISA.A technique was applied to detect early fluorescent antigens (DEFA of cytomegalovirus (CMV using the E13 monoclonal antibodies in 52 immunocompromised patients hospitalized in the Nephrology Institute of Havana. Of the 75 urine or blood (buffy coat samples taken, 15 were found positive to CMV. Using classical diploide human fibroblast isolation technique, 12 CMV strains were isloation of previously detected positive samples by DEFA. In addition, CMV was isolated from one sample reported to be negative by DEFA. A coincidence of 80% was found between both techniques. With the ELISA test, all the sample studied have IgG antibodies to CMV.

  1. Prevalencia de anticuerpos contra dengue y leptospira en la población de Jáltipan, Veracruz Prevalence of dengue and leptospira antibodies in the state of Veracruz, Mexico

    Directory of Open Access Journals (Sweden)

    Joel Navarrete-Espinosa

    2006-06-01

    Full Text Available OBJETIVO: Conocer la prevalencia de anticuerpos contra dengue y leptospira en Jáltipan, Veracruz, México MATERIAL Y MÉTODOS: Estudio transversal en 500 habitantes de Jáltipan, Veracruz, mediante un muestreo por hogares, aplicación de cuestionario y toma de muestras sanguíneas. Se buscaron anticuerpos IgG y se evaluaron factores asociados a sus seroprevalencias mediante regresión logística RESULTADOS: La seroprevalencia global para denguevirus fue 79.6% (IC95% 76-81; los principales factores vinculados con la infección fueron la edad (pOBJECTIVE: To discover the prevalence of antibodies against denguevirus and leptospira. MATERIAL AND METHODS: A cross-sectional study was carried out in 500 inhabitants of Veracruz, Mexico through household sampling, administration of a questionnaire and collection of blood samples. The presence of IgG antibodies was determined and factors associated with seroprevalence were evaluated using a logistic regression model. RESULTS: Total denguevirus seroprevalence was 79.6% (IC95% 76-81. The main factors associated with infection were age and residence in neighborhoods with higher vectorial indices (RMP=2.94, IC95% 1.46-6.1. Overall seroprevalence against leptospira was 4% (IC95% 2-6. Prevalence was higher in the 25 to 44 years age group (35%, IC95% 15-59. Seroprevalence in individuals who referred to living with dogs, pigs, cows and rats was 25% (IC95% 0.63-80. Eighty-five percent of individuals who were leptospira positive were also dengue positive. CONCLUSIONS: The prevalence of denguevirus antibodies suggests the risk of serious dengue hemorrhagic outbreaks. Although leptospirosis prevalence was low, the coexistence of both agents was confirmed.

  2. HBV vaccination of HCV-infected patients with occult HBV infection and anti-HBc-positive blood donors

    Directory of Open Access Journals (Sweden)

    J.S.F. Pereira

    2006-04-01

    Full Text Available Anti-HBc positivity is a frequent cause of donation rejection at blood banks. Hepatitis B virus (HBV infection may also occur in HBsAg-negative patients, a situation denoted occult infection. Similarly, very low levels of HBV-DNA have also been found in the sera of patients with chronic hepatitis C virus (HCV infection, even in the absence of serum HBsAg. Initially we searched for HBV-DNA in serum of 100 blood donors and 50 HCV-infected patients who were HBsAg negative/anti-HBc positive by nested-PCR and by an HBV monitor commercial test for HBV-DNA. Anti-HBs seroconversion rates were measured in 100 blood donors and in 22 patients with chronic HCV infection after HBV vaccination to determine if the HBV vaccination could eliminate an occult HBV infection in these individuals. Occult HBV infection was detected in proportionally fewer blood donors (6/100 = 6% than chronic hepatitis C patients (12/50 = 24% (P 0.05. All subjects who were HBV-DNA(+ before the first dose of HBV vaccine (D1, became HBV-DNA(- after D1, D2, and D3. Among 22 HCV-positive patients, 10 HBV-DNA(+ and 12 HBV-DNA(-, seroconversion was observed in 9/10 (90% HBV-DNA(+ and in 9/12 (75% HBV-DNA(- subjects (P > 0.05. The disappearance of HBV-DNA in the majority of vaccinated patients suggests that residual HBV can be eliminated in patients with occult infection.

  3. HBsAg、HBeAg定量检测在慢性乙型肝炎抗病毒治疗中的临床意义%Clinical signiifcance of HBsAg and HBeAg quantitative detection in chronic hepatitis B antiviral treatment

    Institute of Scientific and Technical Information of China (English)

    李勤英

    2013-01-01

    Objective To study the clinical significance of HBsAg and HBeAg quantitative detection in antiviral treatment. Methods Total of 152 patients with chronic hepatitis B selected from the author’s hospital received antiviral treatment with interferon and nucleoside analogues. After 12 weeks, 24 weeks and 52 weeks of treatment, quantitative detections of HBsAg, HBeAg and HBV DNA have been carried out so as to observe the correlation between the quantitative detection and the treatment effect. Results For the interferon treatment group:the changes of HBeAg after 12 weeks and HBeAg after 24 weeks have correlation with long-term outcome, which is of statistical signiifcance (both P<0.05);for the nucleoside analogues treatment group:HBsAg quantitative didn’t change signiifcantly in the course of treatment, and the changes of HBeAg after 12 weeks and 24 weeks have been found to be associated with the prognosis of the patients, which also has statistical signiifcance (both P<0.05);HBeAg can serve as a reference index for forecasting the risk of drug resistance. Conclusions HBsAg and HBeAg are two stable and reliable biological markers;the quantitative detection can visually analyze the patients’ conditional changes, predict therapeutic response and help formulate the individualized treatment plan.%目的:探讨HBsAg、HBeAg定量检测在CHB抗病毒治疗中的临床意义。方法选择本院门诊及住院CHB患者152例,应用干扰素、核苷(酸)类似物抗病毒治疗,在治疗前、12周、24周、52周定量检测HBsAg、HBeAg、HBV DNA定量,观察HBsAg、HBeAg与治疗疗效的相关性。结果干扰素治疗组12周HBeAg的变化及24周HBsAg变化与治疗远期效果有相关性,差异均有统计学意义(P均<0.05);核苷类似物治疗组HBsAg定量在治疗过程中变化不明显,12周、24周HBeAg变化与患者预后有关,差异均有统计学意义(P均<0.05),HBeAg可做为预测耐药风险的参考指标。

  4. Serum concentration of sFas and sFasL in healthy HBsAg carriers, chronic viral hepatitis B and C patients

    Institute of Scientific and Technical Information of China (English)

    Tadeusz Wojciech Lapinski; Oksana Kowalczuk; Danuta Prokopowicz; Lech Chyczewski

    2004-01-01

    AIM: To estimate the amount of apoptosis among healthy HBsAg carriers, patients with chronic HBV infection treated with lamk udine and patients with chronic HCV infection treated with interferon alpha and ribavirin. Activity of apoptosis was evaluated by serum sFas/sFasL concentration measurement.Moreover dependence between apoptosis and HBV-DNA or HCV-RNA levels was studied.METHODS: Eighty-six persons were included into study: 34healthy HBsAg carriers, 33 patients with chronic HBV infection and 19 patients with chronic HCV infection. Serum levels of sFas/sFasL were measured by ELISA assay. HBV-DNA and HCV-RNA were measured by RT-PCR assay. Levels of sFas/sFasL were determined before and 2 and 12 wk after therapy in patients with chronic hepatitis B and C infection.HBV-DNA or HCV-RNA was detected before treatment and 6 mo after treatment.RESULTS: Twenty-four (71%) healthy HBsAg carriers showed HBV-DNA over 105/mL, which was comparable to the patients with chronic hepatitis B. Independently from HBV-DNA levels,the concentration of sFas among healthy HBsAg carriers was comparable to healthy persons. Among patients with chronic hepatitis B and C, the concentration of sFas was significantly higher in comparison to healthy HBsAg carriers and healthy persons. In chronic hepatitis B patients the concentration of sFas was decreased during lamivudine treatment. Among chronic hepatitis C patients the concentration of sFas was increased during IFN alpha and ribavirin treatment. sFasL was not detected in control group. Furthermore sFasL occurred more frequently in chronic hepatitis C patients in comparison to chronic hepatitis B patients.CONCLUSION: There are no correlations between apoptosis and HBV-DNA levels. However ther is an association between apoptosis and activity of inflammation in patients with chronic HBV infection. Apoptosis can be increased in patients with chronic hepatitis C by effective treatment which may be a result of apoptosis stimulation by IFN-α.

  5. Evaluación en animales del efecto protector de una inmunoglobulina anti Pseudomonas aeruginosa para uso terapéutico

    Directory of Open Access Journals (Sweden)

    Bárbara Cedré

    2007-04-01

    Full Text Available Pseudomonas aeruginosa constituye uno de los agentes patógenos oportunistas de mayor frecuencia de aislamiento en los diversos procesos infecciosos, por lo que es reconocido como un gran problema de salud a nivel mundial. Al no existir un fármaco de alta efectividad ni vacunas disponibles contra esta bacteria, se emplea una terapia con inmunoglobulinas polivalentes comerciales que de forma combinada con los antibióticos contribuyen a eliminar la infección, aunque los preparados disponibles en el mercado no contienen concentraciones suficientemente elevadas de anticuerpos específicos contra este microorganismo. En este trabajo se llevó a cabo la evaluación en un modelo animal de una inmunoglobulina anti- Pseudomonas aeruginosa para uso terapéutico mediante un ensayo de reto con una cepa virulenta. Se evaluó dosis y vía de administración de la misma, así como el valor profiláctico o terapéutico de los anticuerpos. Esta gammaglobulina resultó ser protectora en animales mostrando una sobreviviencia cercana a un 75% en comparación con el grupo control no protegido y además se logra eliminar el estado de portador en los individuos infectados.

  6. Clinical Research of Enzyme Linked Immunosorbent Assay for The Detection of Hepatitis B Virus Surface Antigen False Positive%酶联免疫法检测HBsAg假阳性临床研究

    Institute of Scientific and Technical Information of China (English)

    张海军

    2013-01-01

    目的:研究分析酶联免疫法(ELISA)检测乙肝病毒表面抗原(HBsAg)的假阳性情况.方法:对酶联免疫法检测HBsAg结果为阳性的1500例血清标本用金标法验证,并用化学发光仪微粒子捕捉免疫发光法(MEIA)定量检测HBsAg的含量,以确认酶联免疫法检测结果的假阳性.结果:1500例酶联免疫法检测HBsAg阳性的血清标本,其中1479例为真阳性,真阳性率为98.6%(1479/1500);21例为假阳性,假阳性率为1.4%(21/1500).结论:酶联免疫法检测HBsAg有一定的假阳性,临床检测时应高度注意,避免错报误诊.%Objective: To study enzyme linked immunosorbent assay ( ELISA ) in detecting hepatitis B virus surface antigen ( HBsAg ) of the false positive cases. Method: ELISA for detection of HBsAg results for 1500 cases with positive serum samples using the colloidal gold method validation, and using chemical luminous instrument microparticle enzyme lmmunoassay ( MEIA ) for quantitative detection of HBsAg content, to confirm the enzyme-linked immunosorbent assay for detection of false positive results. Result: 1500 cases of enzyme-linked immunosorbent assay for detection of HBsAg positive serum samples, including 1479 cases of true positive, true positive rate was 98. 6% ( 1479/1500 ); 21 were false positive, false positive rate was 1. 4% ( 21/1500 ). Conclusion: Enzyme-linked immunosorbent assay for detection of HBsAg have false positive, clinical test should be highly attention, avoid the error diagnosis.

  7. Cloning, Eukaryotic Expression of Human ISG20 and Preliminary Study on the Effect of Its Anti-HBV

    Institute of Scientific and Technical Information of China (English)

    Youhua HAO; Dongliang YANG

    2008-01-01

    Human ISG20 gene was cloned and the effect of its anti-HBV was primarily studied. The ISG20 gene was amplified from HeLa cells by RT-PCR and recombinant vector expressing ISG20 was constructed by genetic engineering. The overexpression of ISG20 in HepG2 cells was detected by Western blot and the levels of secretion of HBs antigen and HBe antigen tested by ELISA. The results showed that: (1) Sequence of ISG20 cloned was consistent to that published in Genebank; (2) Recombinant vector expressing ISG20 could be expressed in HepG2 cells by transfection; (3) The overexpression of ISG20 protein could reduce the levels of the secretion of HBs antigen and HBe an-tigen in transfected HepG2 cells. It was suggested that the overexpression of recombinant ISG20 in culture cells could reduce the synthesis of HBV proteins.

  8. Hepatitis B vaccination with or without hepatitis B immunoglobulin at birth to babies born of HBsAg-positive mothers prevents overt HBV transmission but may not prevent occult HBV infection in babies: a randomized controlled trial.

    Science.gov (United States)

    Pande, C; Sarin, S K; Patra, S; Kumar, A; Mishra, S; Srivastava, S; Bhutia, K; Gupta, E; Mukhopadhyay, C K; Dutta, A K; Trivedi, S S

    2013-11-01

    infection with good immune response 28%. Women who were anti-HBe positive were a low-risk group, and their babies were most likely to remain free of HBV infection (occult or overt) and had good immune response to the vaccine. Maternal HBeAg-positive status and negativity for anti-HBe predicted not only overt but also any infection (both overt and occult) in babies. In addition, high maternal HBV DNA and treatment with vaccine alone were significant factors for overt HBV infection in babies. The current practice of administration of vaccine with HBIG at birth to babies born of HBsAg-positive mothers is not effective in preventing occult HBV infection in babies, which may be up to 40%. Because the most important risk factors for mother-to-baby transmission of HBV infection are the replicative status and high HBV DNA level in mothers; it will be worthwhile investigating the role of antivirals and HBIG administration during pregnancy to prevent mother-to-child transmission of HBV infection. PMID:24168259

  9. Construction of eukaryotic expression plasmids for HbsAg and HSV-2gD antigens%HBsAg、HSV-2gD双抗原真核表达载体的构建

    Institute of Scientific and Technical Information of China (English)

    郑绮菡; 王玉; 焦凤萍; 于爱莲; 于广福

    2012-01-01

    目的 利用简并引物PCR法及重叠PCR法构建HBsAg(S),HSV-2gD模拟抗原表位P6及天然抗原表位NP6,IL-18真核表达载体,并对其表达能力进行鉴定,为后期疫苗的研制奠定基础.方法 采用简并引物PCR法扩增IL-18-P6(包括P6-IL-18)片段及IL-18-NP6(包括NP6-IL-18);根据GenBank(FJ589066.1)公布的S基因设计引物,扩增获得含有重叠区的S片段(包括S1,S2,S3,S4);采用重叠PCR法扩增三基因融合片段IL-18-P6-S,IL-18-NP6 S,S-P6-IL-18和S-NP6-IL-18,经纯化回收后将其克隆到原核载体pMD 18-T simple vector中,测序正确的目的片段插入真核表达载体pcDNA3.1(-),构建重组质粒pcDNA3.1-IL-18-P6-S,pcDNA3.1-IL-18-NP6-S,pcDNA3.1-S-P6-IL-18和pcD-NA3.1-S-NP6-IL-18,采用间接免疫荧光法检测靶基因的表达情况.结果 成功构建了含有双抗原的真核表达载体pcDNA3.1-IL-18-P6-S,pcDNA3.1-IL-18-NP6-S,pcDNA3.1-S-P6-IL-18和pcDNA3.1-S-NP6-IL-18.脂质体介导真核表达载体转染CHO细胞,间接免疫荧光法检测细胞浆中有黄绿色荧光.结论 构建的真核表达载体pcDNA3.1-IL-18-P6-S,pcDNA3.1-IL-18-NP6-S,pcDNA3.1-S-P6-IL-18和pcDNA3.1-S-NP6-IL-18能在CHO细胞中有效表达.%Objective Degenerate primer PCR and overlapping PCR were used to construct IL-18 eukaryotic expression plasmids for natural epitope NP6 and epitope P6 derived from HBsAg(S) and HSV-2gD. Expression of plasmids was determined, laying the foundation for subsequent research. Methods Fragments of IL-18-P6 (including fragments of PS-IL-18) , and IL-18-NP6 (including NP6-IL-18) were subjected to degenerate primer PCR. Primers were designed in accordance with the S gene in GenBank (FJ589066. 1) , and S fragments containing the overlapping zone (including S1, S2, S3, and S4) were obtained. Fragments of the 4 fusion genes IL-18-P6-S, IL-18-NP6-S, S-P6-IL-18, and S-NP6-IL-18 were amplified by overlapping PCR. purified, and inserted into a pMD-18-T simple vector. Correct sequence fragments were

  10. 乙肝父婴垂直传播的临床观察%Clinical observation on vertical transmission from HBsAg-positive fathers to infants

    Institute of Scientific and Technical Information of China (English)

    陈起燕; 张荣莲; 黄欣欣; 修晓燕; 任坤海; 王梅颖

    2013-01-01

    Objective: To explore the effect of paternal hepatitis B virus (HBV) infection on infants.Methods: The positive status of serum HBeAg, paternal serum HBV DNA load, paternal spermic HBV DNA load, and neonatal umbilical cord blood HBV DNA were observed among 306 families with HBsAg - positive fathers, serum negative - HBVM and negative - HBVDNA mothers.Results: The incidence rates of paternal liver dysfunction was 7.5% ; the incidence rate of HBV infection among neonates was 21.6% (66/306) ; in case group, 81.8% of fathers were found with positive HBeAg, the positive rates of blood HBV DNA and spermic HBV DNA were 100% and 54.2% , respectively, which were 8.7, 2.1, and 34.3 times more than those among HBsAg - negative fathers.Conclusion: Paternal serum HBeAg, HBV DNA, and spermic HBV DNA detections should be included into conventional detection of men with positive HBsAg during antenatal care; necessary, systematic, and effective antiviral therapy among the patients with positive results may play an active and effective role in prevention of vertical transmission from HBsAg - positive fathers to infants.%目的:探讨父亲单方为乙肝病毒(HBV)感染者对子代的影响.方法:对父亲HBsAg阳性,母亲血清HBVM及HBVDNA均为阴性的306个家庭进行父亲血清HBeAg阳性状态、父亲血清HBV-DNA病毒载量、父亲精液HBV-DNA载量及新生儿脐带血HBV-DNA进行临床观察.结果:父亲肝功能异常发生率为7.5%;新生儿HBV感染率为21.6% (66/306);病例组中父亲为HBeAg阳性者达81.8%、血液HBV DNA阳性者达100%、精液HBV DNA阳性者达54.2%,分别是阴性者中发生HBV父婴垂直传播的8.7倍、2.1倍和34.3倍.结论:临床观察结果表明,孕前保健中务必将准父亲的血清HBeAg、HBVDNA、精液HBV DNA列入男性HBsAg阳性者的检测常规,对阳性者进行必要的系统有效的抗病毒治疗将对防范乙肝父婴垂直传播起积极和有效的作用.

  11. Investigation of occult hepatitis B virus infection in anti-hbc positive patients from a liver clinic.

    Directory of Open Access Journals (Sweden)

    Maria Carmela Martinez

    Full Text Available Occult hepatitis B infection (OBI is manifested by presence of very low levels (<200IU/mL of Hepatitis B viral DNA (HBV DNA in the blood and the liver while exhibiting undetectable HBV surface antigen (HBsAg. The molecular mechanisms underlying this occurrence are still not completely understood. This study investigated the prevalence of OBI in a high-risk Australian population and compared the HBV S gene sequences of our cohort with reference sequences. Serum from HBV DNA positive, HBsAg negative, and hepatitis B core antibody (anti-HBc positive patients (study cohort were obtained from samples tested at SEALS Serology Laboratory using the Abbott Architect, as part of screening and diagnostic testing. From a total of 228,108 samples reviewed, 1,451 patients were tested for all three OBI markers. Only 10 patients (0.69% out of the 1,451 patients were found to fit the selection criteria for OBI. Sequence analysis of the HBV S gene from 5 suspected OBI infected patients showed increased sequence variability in the 'a' epitope of the major hydrophilic region compared to reference sequences. In addition, a total of eight consistent nucleotide substitutions resulting in seven amino acid changes were observed, and three patients had truncated S gene sequence. These mutations appeared to be stable and may result in alterations in HBsAg conformation. These may negatively impact the affinity of hepatitis B surface antibody (anti-HBs and may explain the false negative results in serological HBV diagnosis. These changes may also enable the virus to persist in the liver by evading immune surveillance. Further studies on a bigger cohort are required to determine whether these amino acid variations have been acquired in the process of immune escape and serve as markers of OBI.

  12. PIKA as an Adjuvant Enhances Specific Humoral and Cellular Immune Responses Following the Vaccination of Mice with HBsAg plus PIKA

    Institute of Scientific and Technical Information of China (English)

    Erxia Shen; Li Li; Lietao Li; Lianqiang Feng; Lin Lu; Ziliang Yao; Haixiang Lin; Changyou Wu

    2007-01-01

    An adjuvant is usually used to enhance the immune response induced by vaccines. The choice of adjuvant or immune enhancer determines the effectiveness of the immune response. Currently, aluminium (Alum, a generic term for salts of aluminium) is the only FDA-approved adjuvant. Alum predominantly induces the differentiation of Th2 cells and thus mediates an antibody immune response. Therefore, there is an urgent need for new adjuvants that enhance not only humoral but also cellular immune responses. In the present study, we demonstrates that PIKA (a stabilized dsRNA) as an adjuvant directly induces the activation and the proliferation of both B and NK cells in vitro. Injection of PIKA into mice results in the production of cytokines in vivo. In addition, the study demonstrates that PIKA promotes the maturation of bone marrow-derived dendritic cells (BMDCs) including up-regulation of the co-stimulatory molecules CD80, CD86 and CD40, and the induction of cytokines such as IL-12p70, IL-12p40 and IL-6. Importantly, after immunization of mice with HBsAg plus PIKA, the presence of PIKA enhances the titers of HBsAg-specific IgG and HBsAg-specific IFN-γ production. These results demonstrate that PIKA as an adjuvant can promote both humoral and cellular immune responses. These might have an implication in applying PIKA as an adjuvant to be used in the design and development of both therapeutic and preventive vaccines, and used in the clinical study.

  13. Vaccine self-assembling immune matrix is a new delivery platform that enhances immune responses to recombinant HBsAg in mice.

    Science.gov (United States)

    Grenfell, Rafaella F Q; Shollenberger, Lisa M; Samli, E Farah; Harn, Donald A

    2015-03-01

    Vaccination remains the most effective public health tool to prevent infectious diseases. Many vaccines are marginally effective and need enhancement for immunocompromised, elderly, and very young populations. To enhance immunogenicity, we exploited the biphasic property of the (RADA)4 synthetic oligopeptide to create VacSIM (vaccine self-assembling immune matrix), a new delivery method. VacSIM solution can easily be mixed with antigens, organisms, and adjuvants for injection. Postinjection, the peptides self-assemble into hydrated nanofiber gel matrices, forming a depot with antigens and adjuvants in the aqueous phase. We believe the depot provides slow release of immunogens, leading to increased activation of antigen-presenting cells that then drive enhanced immunogenicity. Using recombinant hepatitis B virus surface antigen (rHBsAg) as a model immunogen, we compared VacSIM delivery to delivery in alum or complete Freund's adjuvant (CFA). Delivery of the rHBsAg antigen to mice via VacSIM without adjuvant elicited higher specific IgG responses than when rHBsAg was delivered in alum or CFA. Evaluating IgG subtypes showed a mixed Th1/Th2 type response following immunization with VacSIM, which was driven further toward Th1 with addition of CpG as the adjuvant. Increased specific IgG endpoint titers were observed in both C57BL/6 and BALB/c mice, representative of Th1 and Th2 environments, respectively. Restimulation of splenocytes suggests that VacSIM does not cause an immediate proinflammatory response in the host. Overall, these results suggest that VacSIM, as a new delivery method, has the potential to enhance immunogenicity and efficacy of numerous vaccines. PMID:25609075

  14. Perfil infectológico de poblaciones mapuche de Cerro Policía y Aguada Guzmán de la provincia de Río Negro

    Directory of Open Access Journals (Sweden)

    Rey, Jorge Alberto

    1996-01-01

    Full Text Available Nuestro trabajo analiza la prevalencia de marcadores de algunas infecciones que afectan las poblaciones mapuche de Cerro Policía (CP y Aguada Guzmán (AG en la Prov. de Río Negro. Los estudios incluídos son: determinación de anticuerpos anti-Treponema pallidum, anti-Trypanosoma cruzii, anti-Echinococcus granulosus, antígeno de superficie de la Hepatitis B (HBsAg, anticuerpos contra el core de la Hepatitis B (anti-HBc, anticuerpos contra el virus de la Hepatitis A (anti-HAV. Se estudiaron 160 muestras en CP y 83 en AG. Los resultados muestran alta prevalencia para anti-HAV en CP (89.2% y en AG (77.1% y para hidatidosis (10.6% y 9.6% respectivamente. Se hallaron valores nulos o bajos para sífilis (CP: 0.0 % y AG:2.4%. La prevalencia para Chagas fue del 10% en CP y 0.0% en AG. Los datos para Hepatitis B fueron nulos o bajos: HBsAg (CP:0.0% y AG:1.2% y anti-HBc(CP:0.6% y AG:0.0%. Los resultados indican que, aún estando estas dos poblaciones tan próximas muestran diferencias en los perfiles de los marcadores serológicos estudiados. Al comparar las tasas de prevalencia con las de otras poblaciones de América, cada una presenta un perfil diferente que depende de la población y del agente infeccioso considerado.

  15. A novel β-globin gene mutation HBB.c.22 G>C produces a hemoglobin variant (Hb Vellore) mimicking HbS in HPLC.

    Science.gov (United States)

    Edison, E S; Sathya, M; Rajkumar, S V; Nair, S C; Srivastava, A; Shaji, R V

    2012-10-01

    Hemoglobinopathies are highly prevalent in Indian population. DNA analysis to detect causative mutations is required for identifying rare hemoglobin variants or when hematological results are discordant with the clinical phenotype. In this report, we describe a novel hemoglobin variant caused by a mutation in beta-globin gene, Codon 7 GAG→CAG (Glu→Gln) that elutes in the position of sickle haemoglobin (HbS) in cation exchange high performance liquid chromatography. This report highlights possible diagnostic pitfalls in interpreting data solely based on haemoglobin analysis and usefulness of mutation screening in definitive diagnosis of hemoglobinopathies. PMID:22471768

  16. HBsAg及B7-2抗原重组腺病毒载体感染免疫研究%Humoral immunization and cell-mediated immunization evoked by HBsAg and B7-2 Ag coexpression recombinant adenovirus vector

    Institute of Scientific and Technical Information of China (English)

    周智; 张定凤; 任红

    2001-01-01

    目的为激发机体对HBsAg的CTL反应,寻求对慢性乙型肝炎更有效的治疗方法。方法构建了共表达HBsAg及B7-2抗原的E1区插入重组腺病毒载体,用脂质体法转染293细胞,经空斑筛选表达目的抗原的重组腺病毒,用ELISA法及Western blotting法分别检测HBsAg及B7-2抗原表达。在293细胞中扩增,再纯化、浓缩后,体内感染C57小鼠,检测体液免疫反应和细胞免疫反应。结果重组腺病毒在体外培养的293细胞及HepG2细胞中高效表达HBsAg及B7-2抗原。感染小鼠体液免疫较弱,但可通过注射乙型肝炎疫苗而加强;重组腺病毒载体能诱导强而有效的细胞免疫反应;未观察到明显毒副作用。结论 HBsAg及B7-2抗原重组腺病毒载体体外感染293、HepG2细胞后高效表达目的抗原,感染免疫后诱导有效的细胞免疫反应。初步结果显示腺病毒载体是一种高效、安全的载体系统。%Objective To evoke cytotoxic T lymphocytes (CTL) response and seek for a more effective method to treat chronic hepatitis B. Methods The adenovirus vector was constructed with the foreign genes inserted in the early region 1(E1), which directed coexpression of HBV-S and B7-2 antigens by means of an internal ribosomal entry site placed between the two coding sequences. The vector was transfected into 293 cell lines by liposome and the adenovirus expressing the target antigens was obtained by plaque select. The HBsAg and B7-2 antigen expression in in vitro cell culture was measured by ELISA and Western blotting, respectively. The immune responses were measured by ELISA for antibody response and a LDH release assay for CTL activity after immunization with the recombinant adenovirus vector in C57 mice. Results HBsAg and B7-2 antigens were highly expressed after infecting the 293 and HepG2 cell lines in vitro. The humoral response to hepatitis B surface antigen was mildly induced and could be enhanced by reinjecting a

  17. Dynamic expression profile of HBsAg according to hepatic parenchyma cells' volume at different liver fibrosis stages in the immune clearance phase%慢性乙型肝炎不同肝纤维化分期的肝实质细胞体积所分摊的HBsAg表达变化

    Institute of Scientific and Technical Information of China (English)

    邬喆斌; 曹红; 刘婷; 吴泽倩; 柯伟民; 高志良

    2012-01-01

    Objective The aim of this study was to determine the dynamic expression profile of hepatitis B surface antigen (HBsAg) according to hepatic parenchyma cells' volume at different stages of liver fibrosis during the immune clearance phase.Methods Eighty-nine patients with HBeAg-positive chronic hepatitis B (CHB) in the immune clearance stage were recruited for study.Each patient's serum HBsAg levels were detected by electrochemiluminescence.The serum HBsAg levels were apportioned according to hepatic parenchyma cells' volume at liver fibrosis stages 1,2,3,and 4 and compared by ANOVA.Results The unapportioned serum HBsAg levels (IU/mL) at liver fibrosis stages 1 (227.2 ± 237.7),2 (211.0 ± 131.4),3 (300.1 ± 144.6),and 4 (278.7 ± 148.8) were not significantly different (all comparisons,P range:0.061~0.759).However,when the serum HBsAg levels were apportioned by the same hepatic parenchyma cells' volume at liver fibrosis stages 1 (343.9 ± 359.8),2 (336.4 ± 209.5),3 (508.7 ± 245.1),and 4 (525.2 ± 274.8),the levels were significantly different (all comparisons,F =3.045 and P =0.033; stage 1 vs.3,P =0.041;stage 1 vs.4,P =0.046; stage 2 vs.3,P =0.028; stage 2 vs.4,P =0.034).Conclusion During the immune clearance phase of chronic hepatitis B,increased HBsAg expression is associated with increased hepatic parenchyma cells' volume and progressive liver fibrosis stage.%目的 观察慢性乙型肝炎免疫清除期肝纤维化S1、S2、S3和S4期,相同肝实质细胞体积所分摊HBsAg表达的动态变化. 方法 对89例HBeAg阳性慢性乙型肝炎免疫清除期患者应用电化学发光法检测肝纤维化S1、S2、S3和S4期血清HBsAg的水平;进一步用相同肝实质细胞体积分摊并且两两比较肝纤维化S1、S2、S3和S4期血清HBsAg的水平.使用SPSS 15.0统计软件,组间用两两比较,采用ANOVA检验进行分析. 结果 肝纤维化S1、S2、S3和S4期的血清HBsAg水平分别为(227.2±237.7)IU/ml、(211.0±131.4)IU/ml、(300.2

  18. HBsAg quantification in prediction of the long-term efficacy of interferon for chronic hepatitis B%HBsAg定量检测对干扰素治疗慢性乙型肝炎远期疗效的预测价值

    Institute of Scientific and Technical Information of China (English)

    杨阳; 杨龙; 蒋雪花; 陈志勇

    2015-01-01

    目的:探讨血清乙肝表面抗原(HBsAg)定量检测在预测干扰素治疗HBeAg 阳性慢性乙型肝炎远期疗效中的价值。方法:对75例HBeAg 阳性慢性乙型肝炎患者采用聚乙二醇干扰素α-2a,180μg,皮下注射,每周1次,连续48周后停用,完成治疗后随访24周;治疗及随访期间定期检测血清HBsAg 及HBV DNA水平变化。结果:治疗结束后随访24周,根据血清HBV DNA 变化情况将患者分为病毒学持续应答组、无应答组及复发组。在治疗第12、24及48周,持续应答组与复发组相比,血清HBV DNA 水平差异无显著性,且均明显低于无应答组;而在治疗第12、24及48周,持续应答组血清HBsAg 定量检测水平明显低于复发组,复发组与无应答组差异无显著性。在治疗第12周,血清HBsAg 水平下降幅度≥1 log10 IU/mL 对预测持续病毒学应答的敏感性、特异性、阳性预测值及阴性预测值分别为86%、94%、95%及94%;通过受试者工作特征曲线评估治疗第12周血清HBsAg 水平下降幅度≥1 log10 IU/mL 对预测患者病毒学持续应答的价值,得出的曲线下面积为0.952。结论:血清HBV DNA 水平的变化不能有效预测治疗结束后患者能否达到持续病毒学应答,但血清HBsAg 定量检测可作为预测干扰素治疗HBeAg 阳性慢性乙型病毒性肝炎患者疗效的理想指标。%Objective To explore the value of serum HBsAg quantification in prediction of interferon treatment of HBeAg-positive chronic hepatitis B (CHB). Methods 75 patients with HBeAg-positive CHB received peginterferon alfa-2a (PegIFNα-2a) at a dose of 180 μg weekly for 48 weeks with 24 weeks of a pose-treatment follow-up. Serum HBsAg quantification and level of HBV DNA were measured during the treatment and the pose-treatment follow-up. Results In the post-treatment follow-up , the patients were divided into sustained response (SR) group, relapse group

  19. Anticuerpos circulantes contra Influenza Aviar y Newcastle en zanates (Quiscalus mexicanus de la ciudad de Guatemala (Avian flu and Newcastle Antibodies in Great-tailed Grackles (Quiscalus mexicanus in Guatemala city.

    Directory of Open Access Journals (Sweden)

    Escobar, Luis E.

    2011-03-01

    Full Text Available ResumenAunque el zanate (Quiscalus mexicanus está distribuido en todaGuatemala y se encuentra estrechamente ligado a poblaciones humanas,se sabe muy poco acerca de su estado sanitario y su papel comoreservorio de enfermedades aviares de importancia. Esta investigacióngenera información sobre el papel del zanate en la epizootiología de la Influenza Aviar (IA; Orthomyxoviridae y de Newcastle (ENC;Paramixoviridae, midiendo los anticuerpos circulantes en zanates norelacionados a granjas avícolas. Se colectaron 71 muestras de suero dezanates urbanos asintomáticos del la ciudad de Guatemala entre abril yjulio de 2008 y se analizaron con la prueba de Inhibición de laHemoaglutinación.SummaryDespite the Great-tailed Grackle (Quiscalus mexicanus is distributed in all Guatemala and it is so close to human populations, few is knownabout its sanitarian status and role as reservoir of important aviandiseases. This research provides information about the role of the Greattailed Grackle in the epizootiology of Avian Influenza (AI;Orthomyxoviridae and Newcastle Disease (NCD; Paramixoviridae,measuring the antibodies of Grackles non-related to avian farms. Wecollected 71 serum samples from urban Great-tailed Grackles without any symptoms in Guatemala City from April to June of 2008, and analyzed with hemagglutination Inhibition Test.

  20. HBsAg人VH基因中异常序列对抗体活性影响的研究

    Institute of Scientific and Technical Information of China (English)

    陈晓穗; 朱迎春; 正欲晓; 王琰; 顾维丰

    2000-01-01

    目的:我们曾从噬菌体抗体库中克隆到一株VH第3骨架区中含有异常序列的抗HBsAg人Fab基因,本工作拟探讨该VH基因中异常序列对抗体活性的影响.方法:采用重叠PCR方法去除这段异常序列,构建表达载体,转化大肠杆菌表达出抗HBsAg噬菌体抗体和可溶性Fab,测定了它们与抗原的结合活性.结果:与原抗体比较,改造后的抗体与抗原的结合活性明显下降,分子模建提示这段异常序列对VH CDR1和CDR2的部分氨基酸残基的位置有一些影响,测定改造前Fab的亲和力约为0.55×109M-1,改造后Fab因抗原结合活性过低,无法检测其亲和力.结论:提示这段异常序列在体内就可能存在于该VH之中.

  1. Heterogeneous UO2 fuel irradiated up to a high burn-up: Investigation of the HBS and of fission product releases

    Science.gov (United States)

    Noirot, J.; Lamontagne, J.; Nakae, N.; Kitagawa, T.; Kosaka, Y.; Tverberg, T.

    2013-11-01

    A UO2 fuel with a heterogeneous distribution of 235U was irradiated up to a high burn-up in the Halden Boiling Water Reactor (HBWR). The last 100 days of irradiation were performed with an increased level of linear power. The effect of the heterogeneous fissile isotope distribution on the formation of the HBS was studied free of the possible influence of Pu which exists in heterogeneous MOX fuels. The HBS formed in 235U-rich agglomerates and its main characteristics were very similar to those of the HBS formed in Pu-rich agglomerates of heterogeneous MOX fuels. The maximum local contents of Nd and Xe before HBS formation were studied in this fuel. In addition to a Pu effect that promotes the HBS phenomenon, comparison with previous results for heterogeneous MOX fuels showed that the local fission product concentration was not the only parameter that has to be taken into consideration. It appears that the local actinide depletion by fission and/or the energy locally deposited through electronic interactions in the fission fragment recoils also have an effect on the HBS formation threshold. Moreover, a major release of fission gases from the peripheral 235U-rich agglomerates of HBS bubbles and a Cs radial movement are also evidenced in this heterogeneous UO2. Cs deposits on the peripheral grain boundaries, including the HBS grain boundaries, are considered to reveal the release paths. SUP>235U-rich agglomerates, SUP>235U-poor areas, an intermediate phase with intermediate 235U concentrations. Short fuel rods were fabricated with these pellets. The main characteristics of these fuel rods are shown in Table 1.These rods were irradiated to high burn-ups in the IFA-609/626 of the HBWR and then one was irradiated in the IFA-702 for 100 days. Fig. 2 shows the irradiation history of this fuel. The final average burn-up of the rod was 69 GWd/tU. Due to the flux differences along the rod, however, the average burn-up of the cross section examined was 63 GWd/tU. This fuel

  2. Radioimmunoassay for the detection of HBeAg and anti-HBe.

    Science.gov (United States)

    Zanetti, A R; Ferroni, P; Bonino, F; Recchia, S; Aricò, S; Palla, M; Caspani, B

    1979-01-31

    We describe a solid-phase radioimmunoassay, based on the "sandwich" principle, using anti-HBe1/anti-HBe2 containing IgG precoated to polystirene beads as the solid-phase, and 125I-anti-HBE as labelled antibody for the detection of HBeAg and a two steps competitive procedure for the detection of anti-HBe. Comparison of titre obtained by ID and RIA or reference sera containing HBeAg and anti-HBe revealed that RIA is about 200 fold more sensitive than ID in detecting both HBeAg and anti-HBe. Moreover an increase in the detection rate of HBeAg from 5.9% (ID) to 15% (RIA) and of anti-HBe from 47.8% (ID) to 74.2% (RIA) and consequently a reduction of the frequency of negative samples for both HBeAg and anti-HBe from 46.3% (ID) to 10.8% (RIA) was determined by testing sera from HBsAg asymptomatic carriers. With regard to specificity, the frequency of presumptive HBeAg positive samples not confirmed was of 1 out of 29 (3.4%). Technical improvements of sensitivity and specificity of the described RIA are under development. PMID:95390

  3. Anti-inflammatory and antiviral effects of Glossogyne tenuifolia.

    Science.gov (United States)

    Wu, Ming-Jiuan; Weng, Ching-Yi; Ding, Hsiou-Yu; Wu, Pei-Jong

    2005-01-21

    Glossogyne tenuifolia (Hsiang-Ju) is a traditional antipyretic and hepatoprotective herb used in Chinese medicine. The aim of this research is to investigate the pharmacological activities and potent components of the ethanol extract of Glossogyne tenuifolia (GT) in human primary cells and cell line. We found that GT (0.1 approximately 0.25 mg/ml) exerted dose-dependent inhibitions on the release of TNF-alpha and IL-6 in LPS-activated human whole blood and peripheral blood mononuclear cells (PBMC), and IFN-gamma in PHA-stimulated human whole blood. The lack of cytotoxicity indicated that the inhibitory effects of GT on cytokine production were not due to cell death. Luteolin, the deglycosylated derivative of one of the major compositions, luteolin-7-glucoside, exerted inhibitory effects on TNF-alpha, IL-6 and IFN-gamma production in activated human whole blood with estimated IC(50)s of 42.73 microM, 44.86 microM and 3.34 microM, respectively. Furthermore, GT had potent anti-hepatitis B virus (HBV) effects on the human hepatocellular carcinoma cell line, PLC/PRF/5. GT exhibited a dose-dependent inhibition on the release of hepatitis B surface antigen (HBsAg) by repressing the expression of HBsAg with IC(50) of 0.093 mg/ml. We concluded that GT exerted combinatorial anti-inflammatory and antiviral effects, and the multiple actions may underlie its traditional hepatoprotective function. PMID:15620577

  4. Seroprevalence of hepatitis B surface antigen and anti HCV antibody and its associated risk factors among pregnant women attending maternity ward of Felege Hiwot Referral Hospital, northwest Ethiopia: a cross-sectional study

    OpenAIRE

    Molla, Sefinew; Munshea, Abaineh; Nibret, Endalkachew

    2015-01-01

    Background Viral hepatitis is a life-threatening liver disease that has become important public health issue in developing countries including Ethiopia. This study was undertaken to determine the seroprevalence of HBsAgs and anti-HCV antibodies and what socio-demographic factors are associated with sero-positivity of Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) infections among pregnant women attending maternity ward of Felege Hiwot Referral Hospital, northwest, Ethiopia. Methods Hospi...

  5. Association of variants at BCL11A and HBS1L-MYB with hemoglobin F and hospitalization rates among sickle cell patients in Cameroon.

    Directory of Open Access Journals (Sweden)

    Ambroise Wonkam

    Full Text Available BACKGROUND: Genetic variation at loci influencing adult levels of HbF have been shown to modify the clinical course of sickle cell disease (SCD. Data on this important aspect of SCD have not yet been reported from West Africa. We investigated the relationship between HbF levels and the relevant genetic loci in 610 patients with SCD (98% HbSS homozygotes from Cameroon, and compared the results to a well-characterized African-American cohort. METHODS AND FINDINGS: Socio-demographic and clinical features were collected and medical records reviewed. Only patients >5 years old, who had not received a blood transfusion or treatment with hydroxyurea were included. Hemoglobin electrophoresis and a full blood count were conducted upon arrival at the hospital. RFLP-PCR was used to describe the HBB gene haplotypes. SNaPshot PCR, Capillary electrophoresis and cycle sequencing were used for the genotyping of 10 selected SNPs. Genetic analysis was performed with PLINK software and statistical models in the statistical package R. Allele frequencies of relevant variants at BCL11A were similar to those detected in African Americans; although the relationships with Hb F were significant (p <.001, they explained substantially less of the variance in HbF than was observed among African Americans (∼ 2% vs 10%. SNPs in HBS1L-MYB region (HMIP likewise had a significant impact on HbF, however, we did not find an association between HbF and the variations in HBB cluster and OR51B5/6 locus on chromosome 11p, due in part to the virtual absence of the Senegal and Indian Arab haplotypes. We also found evidence that selected SNPs in HBS1L-MYB region (HMIP and BCL11A affect both other hematological indices and rates of hospitalization. CONCLUSIONS: This study has confirmed the associations of SNPs in BCL11A and HBS1L-MYB and fetal haemoglobin in Cameroonian SCA patients; hematological indices and hospitalization rates were also associated with specific allelic variants.

  6. Neospora caninum antibodies in dairy cattle of Lages Municipality, Santa Catarina State, Brazil Anticuerpos séricos contra Neospora caninum en rebaños lecheros de la ciudad de Lages, Estado de Santa Catarina, Brasil

    Directory of Open Access Journals (Sweden)

    AB Moura

    2012-01-01

    Full Text Available Bovines are the main intermediary host of the protozoan Neospora caninum, which is a major cause of bovine abortions and neonatal mortality worldwide. Sera were collected from 373 dairy cattle and 33 dogs on 19 dairy farms in Lages city, Santa Catarina State, Brazil, to determine the prevalence of N. caninum antibodies and risk factors. Tests for N. caninum antibodies were done using an indirect immunofluorescent antibody test (IFAT. Positive reactions with titers ≥ 1:200 (cattle and ≥ 1:50 (dogs were found in 86 (23.1% and seven (21.2% bovines and dogs, respectively. Of the bovines, four (1.1% were calves, 33 (8.8% were heifers, 314 (84.2% were lactating cows, 19 (5.1% were dry cows and three (0.8% were bulls. Infection was primarily found in animals with low milk production (P = 0.008. The presence of seropositive dogs on the properties did not affect the prevalence of bovine infection. No correlation between bovine reproductive disorders and the prevalence of N. caninum antibodies was observed. Our results showed that N. caninum infection is widespread among dairy cattle of Lages, Santa Catarina.Los bovinos son los principales hospederos intermediarios del coccidio Neospora caninum. En esta especie se concentran la mayoría de los daños reproductivos. A fin de determinar la prevalencia de N. caninum e identificar factores de riesgo para la infección en el ganado lechero de la ciudad de Lages, Estado de Santa Catarina, se tomaron muestras de sangre de bovinos (n = 373 y los perros (n = 33 en 19 propiedades. La detección de anticuerpos contra N. caninum fue realizada por medio de IFA. Fueron consideradas positivas las muestras con títulos ≥ 200 (ganado bovino y ≥ 50 (perros. Informaciones relacionadas con la categoría de los animales, la raza, la frecuencia y tipo de trastornos de la reproducción, la producción de leche y la presencia de los perros fueron obtenidos mediante un cuestionario. Los datos fueron analizados con la

  7. Estudio del anticuerpo DOG1 en el diagnóstico de tumores del estroma gastrointestinal - GIST The role of the DOG1 antibody in the diagnosis of gastrointestinal stromal tumours - GIST

    Directory of Open Access Journals (Sweden)

    M.R. Mercado

    2011-08-01

    Full Text Available Los tumores del estroma gastrointestinal (GIST poseen mutaciones en los genes del receptor de la tirosín quinasa (RTKs KIT y PDGFRA. La posibilidad de bloquear esta actividad ha significado una nueva esperanza terapéutica. El diagnóstico de GIST recae en la expresión inmunohistoquímica del c-KIT, pero un 4-15% son c-KIT negativos (aún en presencia de mutación, y sin embargo estos pacientes podrían beneficiarse del tratamiento con inhibidores tirosín quinasa (TKIs. El DOG1 es un nuevo anticuerpo cuya sensibilidad y especificidad parece ser superior o igual a la del c-KIT. El objetivo de este trabajo es evaluar la sensibilidad (Se y especificidad (Sp de DOG1 en GIST de tipo usual (c-KIT positivos, de tipo inusual (c-KIT negativos y frente a otros tumores fusocelulares mesenquimales, y comparar la validez diagnóstica del DOG1 frente al c-KIT. Estudiamos 40 GIST, 39 c-KIT positivos y un c-KIT negativo. Se realizó un panel inmunohistoquímico con los anticuerpos: c-KIT, CD34, actina músculo liso, DOG1 y S100, en los GIST como en siete tumores fusocelulares. La Se y Sp de GIST para DOG1 fue del 100 y 97,5% para c-KIT. La inmunoreactividad para DOG1 en todos los tumores fusocelulares fue negativa. La validez diagnóstica de DOG1 y C-KIT fue similar a la hora de detectar GIST y no GIST. DOG1 es un marcador específico y sensible para el diagnóstico y diagnóstico diferencial de GISTs (es capaz de detectar algunos GIST sin mutación en RTK. El DOG1 debería de formar parte del panel inmunohistoquímico para el diagnóstico de GIST.Gatrointestinal stromal tumours (GIST harbour oncogenic mutations in tyrosin kynases receptors (RTKs including KIT and PDGFRA. The inhibition of this activity has been regarded as the primary target for the treatment of these patients. Diagnosis of GIST relies on c-KIT inmunoreactivity; however there is a 4-15% of GISTs that are C-KIT negative which may lead to underdiagnosis of GISTs and possible withholding of

  8. Clinical Evaluation of HBsAg/TP Antibody/HIV Antibody Colloidal Gold Joint Detection Reagent for Blood Screening%HBsAg/TP抗体/HIV抗体胶体金联合检测试剂在献血者筛查中的应用评价

    Institute of Scientific and Technical Information of China (English)

    郑优荣; 林茹; 李仲平; 彭运平; 杨永崧; 熊新灿; 钟俊玲

    2010-01-01

    目的 评价乙型肝炎病毒表面抗原(HBsAg)、梅毒螺旋体抗体(TP抗体)和人类免疫缺陷病毒抗体(HIV抗体)胶体金联合检测试剂的检测灵敏性、特异性和重复性,以及应用于献血筛查的意义.方法 采用胶体金联合检测试剂分别对2 250份正常献血者血液标本、85份HBsAg阳性标本,78份TP抗体阳性标本和62份HIV抗体阳性标本进行检测,并与参比试剂检测结果进行比较.结果 胶体金联合检测试剂与TP抗体参比试剂的总符合率、灵敏度(阳性符合率)和特异性(阴性符合率)分别为99.87%,100%和99.87%;与HBsAg参比试剂的总符合率、灵敏度和特异性分别为99.82%,94.74%和99.87%,与HIV抗体参比试剂的总符合率和特异性为100%,各项检测结果差异均无统计学意义(χ2=0.36,P>0.05;χ2=0.09,P>0.05;χ2=0.08,P>0.05).胶体金联合检测试剂实验重复性阳性符合率为100%.结论 胶体金联合检测试剂具有反应速度快,操作更加简单和快捷、灵敏度高、特异性和重复性好,适用于献血前血液筛查.%Objective To evaluate method sensitivity, specificity and repeatability of the hepatitis B virus surface antigen (HBsAg), Treponema pallidum Antibody (TP antibody) and human immunodeficiency virus antibody (HIV antibody) colloidal gold joint detection reagent and their application significances for blood donation screening. Methods 2 250 blood samples of normal blood donors, 85 samples of HBsAg positive, 78 samples of TP-positive and 62 samples of HIV antibody positive were detected by the colloidal gold reagent respectively, and with reference reagent test results were compared. Results Compared with TP antibody reference reagents, total coincidence rate, sensitivity (the positive coincidence rate) and specificity (negative coincidence rate) of the colloidal gold joint detection reagent were 99. 87% , 100% and 99.87% respectively, and with HBsAg reference reagents were 99. 82%, 94. 74% and 99. 87

  9. Universal screening for hepatitis B among pregnant women led to 96% vaccination coverage among newborns of HBsAg positive mothers in Denmark

    DEFF Research Database (Denmark)

    Harder, Katja Majlund; Cowan, Susan; Eriksen, Mette Brandt;

    2011-01-01

    to examine the effectiveness of universal HBV-screening of pregnant women and HBV-immunizations of their newborn, and to provide a prevalence-estimate for HBV in Denmark. On a opt out basis all women in Denmark attending antenatal care were tested for hepatitis B serology. Vaccination data of the newborns......%) and 13% had a HBVDNA ≥10(8) IU/ml. The prevalence estimate of chronic hepatitis B in Denmark was 0.2-0.3% in the general population. Among children born within the project period, 96% received vaccination at birth compared to 50% of siblings born prior to universal screening. During 3 years of passive......In Denmark selective screening programs of pregnant women for hepatitis B missed 30-50% of high-risk groups and in late 2005 a universal screening of pregnant women for HBsAg was implemented. During a 2-year period a prospective enhanced surveillance of the universal screening was performed...

  10. Evaluation of serum HBsAg, HBcrAg and HBV DNA in prediction of the pathological status of liver in patients with chronic hepatitis B%血清HBsAg、HBcrAg和HBV DNA预测慢性乙型肝炎肝脏病理状态的评价

    Institute of Scientific and Technical Information of China (English)

    张占卿; 陆伟; 翁齐铖; 张智勇; 沈芳; 王雁冰; 冯艳玲

    2016-01-01

    目的:评价血清乙型肝炎病毒表面抗原(HBsAg)、乙型肝炎病毒核心相关抗原(HBcrAg)和乙型肝炎病毒DNA(HBV DNA)预测慢性乙型肝炎(CHB)患者肝脏不同病理学分级和分期的有效性。方法共选取CHB患者211例,其中HBeAg阳性和阴性分别为125例和86例。血清HBsAg、HBeAg和HBcrAg分别采用化学发光微粒子免疫法和化学发光酶免疫法检测,血清HBV DNA采用实时荧光定量PCR检测。结果 HBeAg阳性患者,血清HBsAg、HBcrAg和HBV DNA与肝脏病理学分级和分期均呈显著负相关(P均<0.05);HBeAg阴性患者,血清HBcrAg和HBV DNA与肝脏病理学分级和分期均呈显著正相关(P均<0.01)。Bayes逐步判别分析显示,HBeAg阳性患者,仅血清HBsAg符合判别肝脏不同病理学分级和分期的方程纳入自变量的标准(F >3.84),Fisher判别函数预测肝脏病理学分级G1、G3和分期S1、S4的正确率分别为62.7%、58.8%和63.8%、69.6%;HBeAg阴性患者,血清HBV DNA和HBcrAg分别符合判别肝脏不同病理学分级和分期的方程纳入自变量的标准(F >3.84),Fisher判别函数预测肝脏病理学分级G1、G3和分期S1、S4的正确率分别为79.3%、66.7%和71.7%、75.0%。结论血清HBsAg对HBeAg阳性患者以及血清HBV DNA和HBcrAg分别对HBeAg阴性患者的肝脏病理学分级G1、G3和分期S1、S4有显著的预测意义。%Objective To evaluate the efficacy of serum hepatitis B surface antigen (HBsAg), hepatitis B core-related antigen (HBcrAg) and hepatitis B virus DNA (HBV DNA) in predicting the pathological grading and staging of liver in patients with chronic hepatitis B (CHB). Methods Total of 211 patients with CHB, including 125 cases with HBeAg-positive and 86 cases with HBeAg-negative, were enrolled. The levels of serum HBsAg, HBcrAg and HBV DNA were measured by chemiluminescence microparticle immunoassay, chemiluminescence enzyme immunoassay and real time

  11. Respuesta de anticuerpos inducidos por la vacuna antimeningocócica cubana VA-MENGOC-BC® frente a la cepa de Neisseria meningitidis B:4:P1.19,15 en adolescentes después de 12 años de inmunizados

    Directory of Open Access Journals (Sweden)

    María A. Camaraza

    2006-12-01

    Full Text Available Se estudió la respuesta de anticuerpos inducida por la vacuna antimeningocócica cubana VA-MENGOC-BC® contra la cepa de Neisseria meningitidis B:4:P1.19,15 mediante el Ensayo Bactericida del Suero (EBS y ELISA de tipo indirecto, para medir anticuerpos contra vesículas de membrana externa (VME de N. meningitidis B a 184 adolescentes de un politécnico de Ciego de Ávila que fueron inmunizados en campañas masivas 12 años antes. Se realizaron extracciones de sangre antes de aplicar la primera dosis (T0, 4 semanas después de ésta (T1 y 4 semanas después de la segunda dosis (T2. Transcurridos 12 años de esta vacunación el 42% de los adolescentes presentó títulos bactericidas ≥ 1:4 frente a la cepa homóloga (B:4:P1.19,15 y el 98% mostró anticuerpos detectables contra las VME. En el EBS; el porcentaje de seroconversión T1/T0 fue del 57% y T2/T0 del 60%. Mediante ELISA la seroconversión fue del 59% y 54%, respectivamente, por lo que se demostró que la aplicación de una sola dosis después de 12 años indujo una respuesta inmune importante que puede sugerir una respuesta anamnésica.

  12. Comparison of three different recombinant hepatitis B vaccines: GeneVac-B, Engerix B and Shanvac B in high risk infants born to HBsAg positive mothers in India

    OpenAIRE

    Velu, Vijayakumar; Nandakumar, Subhadra; Shanmugam, Saravanan; Jadhav, Suresh Sakharam; Kulkarni, Prasad Suryakant; Thyagarajan, Sadras Panchatcharam

    2007-01-01

    AIM: To evaluate a low cost Indian recombinant hepatitis B vaccine GeneVac-B® for its immunogenicity and safety in comparison to Engerix B® and Shanvac B® vaccine in high risk newborn infants born to hepatitis B surface antigen (HBsAg) positive mothers.

  13. Activation of a heterogeneous hepatitis B (HB) core and e antigen-specific CD4+ T-cell population during seroconversion to anti-HBe and anti-HBs in hepatitis B virus infection.

    OpenAIRE

    Jung, M C; Diepolder, H M; Spengler, U; Wierenga, E A; Zachoval, R; Hoffmann, R M; Eichenlaub, D; Frösner, G; Will, H; Pape, G. R.

    1995-01-01

    Overcoming hepatitis B virus infection essentially depends on the appropriate immune response of the infected host. Among the hepatitis B virus antigens, the core (HBcAg) and e (HBeAg) proteins appear highly immunogenic and induce important lymphocyte effector functions. In order to investigate the importance of HBcAg/HBeAg-specific T lymphocytes in patients with acute and chronic hepatitis B and to identify immunodominant epitopes within the HBcAg/HBeAg, CD4+ T-cell responses to hepatitis B ...

  14. CpG ODN佐剂促进HBsAg疫苗诱导小鼠细胞免疫应答的探讨%CpG ODN adjuvant enhances the cellular immune response in mice vaccinated with recombinant hepatitis B surface antigen

    Institute of Scientific and Technical Information of China (English)

    马瑞; 黄俊; 吴长有

    2006-01-01

    目的探讨CpG ODN对重组乙型肝炎表面抗原(HBsAg)诱导小鼠细胞免疫应答的影响.方法HBsAg和HBsAg+CpG ODN分别肌肉注射免疫Balb/c小鼠后,从淋巴组织(脾和淋巴结)和非淋巴组织(肺)分离淋巴细胞,体外经HBsAg抗原刺激后,利用ELISA检测细胞培养液中IFN-γ的水平,再利用流式细胞仪在单个细胞水平上检测IFN-γ+细胞的频率,分析IFN-γ+细胞亚群.结果对照组和HBsAg免疫组IFN-γ产生的水平很低,当HBsAg加强免疫1~2次后,IFN-γ表达仍然没有明显升高;而CpG ODN+HBsAg免疫1次或加强免疫后,CpG显著促进HBsAg诱导的IFN-γ产生.进一研究结果表明CpG促进HBsAg诱导淋巴器官和非淋巴器官内CD4+和CD8+T细胞IFN-γ的表达.结论CpG免疫佐剂不仅能诱导细胞免疫应答同时也诱导体液免疫应答,提示CpGODN可以用于HBsAg预防和治疗性佐剂.

  15. Recombinant HBV vaccine enhances the rate of sustained virological response when early initiated after anti-HCV combination therapy.

    Science.gov (United States)

    Hanafy, Amr Shaaban; Farag, Alaa Ahmad; Hassanin, Hassan Mahmoud; Hassaneen, Ahmad Mahmoud

    2016-01-01

    The overall SVR rate for chronic hepatitis C genotype 4 using the Standard of care is 54.3%. HBV infection can be prevented by the administration of effective and safe vaccine. Evaluation of the vaccination-induced anti-HBs response rates in a cohort of HCV Egyptian patients after being exposed to antiviral combination therapy and the magnitude of its effect on the rate of SVR through its putative role in induction of crossed immunity. (A) 500 HCV patients who had completed the course of antiviral therapy and achieved ETR were retrospectively analyzed and received 20 μg of recombinant DNA vaccine for hepatitis B at time intervals (0, 1, and 4 months). The first dose of the vaccine was initiated one month post treatment. (B) Laboratory analysis: Included routine preliminary investigations to anti viral therapy and specific investigations as determination of anti-HBs antibodies 2 months following the third dose of vaccine. 433 patients showed protective response (86.6%), 67 patients were non-responders (13.4%) (P = 0.003). Adding HBV vaccine 1 month post-treatment increased SVR (400 patients, 80%) (χ(2)  = 40.3, P = 0.000). Diabetes affect response to HBV vaccine (P = 0.0001). Adding HBV vaccine to the post treatment care of patients with HCV after termination of antiviral therapy gain two benefits; protection from HBV and significant increase in rates of SVR. PMID:26147509

  16. Guidelines for screening, prophylaxis and critical information prior to initiating anti-TNF-alpha treatment

    DEFF Research Database (Denmark)

    Lassen, Inge Nordgaard; Dahlerup, Jens Frederik; Belard, Erika;

    2012-01-01

    -TNF-alpha agents. Screening should take place for both active tuberculosis and latent tuberculosis. Screening must evaluate the risk of hepatitis B exposure/infection and that of other viral infections such as human immunodeficiency virus (HIV) and varicella zoster virus (VZV). The assessment should include......-rays and laboratory tests, including an interferon gamma release assay, a hepatitis B test, an HIV test and, when prior VZV infection is uncertain, a VZV antibody test. Prophylaxis: Isoniazid should be administered in cases of suspected latent TB infection. Antiviral treatment is recommended in HBs......Ag-positive patients at the start of anti-TNF-alpha treatment. Before anti-TNF-alpha therapy, vaccination with 23-valent pneumococcal vaccine is recommended, and HBV vaccination may be considered in seronegative patients. Annual vaccination against seasonal influenza is recommended. Human papilloma virus vaccination...

  17. Riesgo de enfermedades infecciosas transmitidas por transfusión en el Valle del Cauca, Colombia.

    Directory of Open Access Journals (Sweden)

    Armando Cortés B.

    2009-10-01

    Full Text Available Se informa el potencial de riesgo para enfermedades infecciosas obtenidas a través de transfusión en el Valle del Cauca en 1997. Todas de las unidades de sangre colectadas en este departamento se tamizaron para los siguientes marcadores de infección: anticuerpos contra el virus de la inmunodeficiencia humana (anti-VIH-1,2, anticuerpos contra el virus de la hepatitis C (anti-VHC, antígeno de superficie de la hepatitis B (AgHBs y anticuerpos contra Trypanosoma cruzi (anti-Tc, y parcialmente para anticuerpos no treponémicos (VDRL/RPR, anticuerpos contra el virus linfotrópico de células T (anti-HTLV-I/II y anticuerpos totales contra el "core" de la hepatitis B (anti-HBc. El riesgo de adquirir VIH y hepatitis B fue más bajo que para hepatitis C, HTLV y T. cruzi debido al completo tamizaje serológico y reducida prevalencia. El índice calculado de diseminación para cada enfermedad infecciosa fue mayor valor obtenido para bancos de sangre de Cali y tipo de donación coactiva con 12 y 16 infecciones/10,000 transfusiones, respectivamente. El índice fue más bajo en bancos de sangre situados fuera de Cali y con la donación voluntaria correspondiendo a 10 y 7 infecciones/10,000 transfusiones, respectivamente. A pesar de que el número de unidades potencialmente infectadas o personas infectadas es probablemente más bajo que nuestras estimaciones por los falsos positivos y receptores ya infectados, estos datos refuerzan la necesidad de mantener esta información para evaluar el nivel de tamizaje de enfermedades infecciosas en la sangre disponible para la comunidad. Estos datos también le permite a los médicos usuarios y ordenadores de las transfusiones conocer el riesgo aproximado de inducir iatrogénicamente una infección por transfusión en el Valle del Cauca, elemento útil para considerar en la toma de decisiones y a los epidemiológos y encargados de vigilar la salud, el impacto de las transfusiones en la diseminación de ciertas

  18. Detection of hepatitis B virus DNA in sera from 18 alcoholic carriers of "anti-HBc alone" and response to a single dose of hepatitis B vaccine Pesquisa de DNA do vírus da hepatite B no soro de 18 alcoolistas portadores de "anti-HBc isolado" e resposta a uma dose da vacina para hepatite B

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Marques de Oliveira

    2008-09-01

    Full Text Available To evaluate the possibility of occult hepatitis B virus (HBV infection in alcoholics carriers of "anti-HBc alone", and to verify the behavior of this serological pattern after a single dose of hepatitis B vaccine, 18 alcoholics who had this serological profile were evaluated by the polymerase chain reaction method, and 17 of them were vaccined. All were negative for HBV DNA. Nine (52.9% of those vaccined had anamnestic response, mainly those with positive anti-HBe (8/10; 80%. "Anti-HBc alone" was compatible with low levels of anti-HBs in half of the patients, and probably with false positive results for anti-HBc in the others.Para avaliar a possibilidade de infecção oculta pelo vírus da hepatite B em alcoolistas com "anti-HBc isolado" e a resposta a uma dose da vacina para a hepatite B, 18 alcoolistas com este perfil sorológico foram avaliados pelo método de reação em cadeia da polimerase e 17 deles foram vacinados. Todos tiveram negativos os exames para o VHB DNA. Nove (52,9% dos vacinados tiveram resposta anamnéstica, principalmente aqueles com anti-HBe positivo (8/10; 80%. "Anti-HBc isolado" foi compatível com baixos títulos de anti-HBs em metade dos pacientes, e provavelmente com resultado falso-positivo para o anti-HBc nos demais.

  19. Design, synthesis, molecular docking studies and anti-HBV activity of phenylpropanoid derivatives.

    Science.gov (United States)

    Liu, Sheng; Li, Yubin; Wei, Wanxing; Wang, Kuiwu; Wang, Lisheng; Wang, Jianyi

    2016-05-01

    In this work, a series of phenylpropanoid derivatives were synthesized, and their anti-hepatitis B virus (HBV) activity was evaluated. Most of the synthesized derivatives showed effective anti-HBV activity. And compound 4d-3 showed the most effective anti-HBV activity, performing strong potent inhibitory not only on the secretion of HBsAg (IC50 = 58.28 μM, SI = 23.26) and HBeAg (IC50 = 97.21 μM, SI = 13.95), but also on the HBV DNA replication (IC50 = 42.28 μM, SI = 32.06). The structure-activity relationships (SARs) of the derivatives had been discussed, which were useful for developing phenylpropanoid derivatives as novel anti-HBV agents. Moreover, the docking study of all synthesized compounds inside the HLA-A protein (PDB ID: 3OX8) active site was carried out to explore the molecular interactions and a molecular target for activity and a modified assay method measuring the interaction between our derivatives and HBcAg was investigated, indicating that the HBV core protein might be their potential target for anti-HBV. This study identified a new class of potent non-nucleoside anti-HBV agents.

  20. Evaluación de la calidad de los reactivos que se utilizan en la tecnica de contrainmunoelectroforesis para la determinación de anticuerpos antirrábicos Evaluation of the quality of the reagents employed in the counterimmunoelectrophoresis technique for the determination of rabies antibodies

    Directory of Open Access Journals (Sweden)

    A. M. Díaz

    1991-02-01

    Full Text Available En este estudio se comprobó que el Instituto Butantan produce antígenos y sueros indicadores que se pueden utilizar con éxito en la prueba de contrainmunoelectroforesis para titular anticuerpos antirrábicos en personas inmunizadas. No se pudieron demostrar diferencias estadísticamente significativas entre los resultados de las pruebas de estandarización realizadas en el Instituto Butantan y las pruebas de control de referencia llevadas a cabo en el Centro Panamericano de Zoonosis. Se propone que el Instituto Butantan produzca y distribuya a nivel nacional los reactivos para que los laboratorios de diagnóstico apliquen la técnica de contrainmunoelectroforesis para la determinación de anticuerpos antirrábicos.This study demonstrated that the antigens and indicator sera produced by the Butantan Institute may be employed with success in the counterimmunoelectrophoresis technique for the titration of rabies antibodies in sera from immunized individuals. No statistically significant differences were demonstrated between the results obtained in the standardization tests carried out at the Butantan Institute and the reference control tests performed at the Pan American Zoonoses Center. It is proposed that the Butantan Institute be in charge of the production and distribution of these reagents at the national level.

  1. Study on the risk of HBV infection among spouses of HBsAg carriers%乙型肝炎病毒表面抗原携带者配偶感染乙型肝炎危险性研究

    Institute of Scientific and Technical Information of China (English)

    孙爱武; 毕胜利; 王锋; 王富珍; 张爽; 涂秋风; 邵晓萍; 郑徽; 孙校金

    2013-01-01

    目的 探讨乙型肝炎(乙肝)病毒(HBV)表面抗原(HBsAg)携带者配偶感染HBV危险性,为制定乙肝相关防控策略提供参考依据.方法 选择广东和江西省2006年全国乙肝血清流行病学调查发现的20 ~ 45岁HBsAg携带者及配偶作为病例组,运用病例对照方法按居住地、性别、年龄及婚龄等因素配比选择HBsAg阴性者及配偶为对照组,开展问卷调查和乙肝血清学指标检测.利用PCR方法分析夫妻双方均HBsAg阳性的HBV基因型.结果 HBsAg携带者配偶HBsAg携带率为13.21%,明显高于对照组配偶(6.29%),差异有统计学意义(x2=4.23,P<0.05);HBsAg携带者其配偶的携带率均高于HBsAg阴性者的配偶;HBsAg携带者配偶的HBsAg携带率与结婚年限、性生活频率呈正相关,并与安全性行为关系密切.21对HBsAg均阳性的夫妻中,13对PCR扩增HBV阳性,其中11对(84.62%)HBV基因型相同(8对HBV为B型,3对为C型),仅2对夫妻HBV基因型不同.结论 HBsAg携带者配偶感染HBV风险高;提倡HBsAg携带者主动告知配偶,采取安全性行为或配偶及时接种乙肝疫苗等有效措施.%Objective To investigate the risk of HBV infection among the spouses of hepatitis B virus surface antigen (HBsAg) carriers and to provide a reference for developing strategies on hepatitis B control and prevention.Methods A case-control study including HBsAg carriers aged 20-45 years-old from the nationwide sero-epidemiological survey for Hepatitis B in both Guangdong and Jiangxi provinces in 2006,together with their spouses were selected as case group,while.HBsAg negative persons and their spouses were among the control groups,under the same residencial areas,gender,age and age of marriage to the HBsAg carriers.Questionnaire survey and hepatitis B serological markers detection were carried out,together with the HBV genotype detection among the HBsAg positive couples between husband and wife by PCR.Results Among the spouses of HBsAg carriers

  2. A comparison of difference in psychological stress between normal pregnant women and HbsAg positive pregnant women%乙肝孕产妇与正常孕产妇心理变化及比较

    Institute of Scientific and Technical Information of China (English)

    李亚萍; 郭新贤; 周文勤

    2011-01-01

    Objective To compare difference in psychological stress level between normal pregnant women and pregnant women with positive hepatitis B surface antigen (HbsAg). Methods 69 pregnant women with positive HbsAg (case group) and 123 normal pregnant women (control group) were randomly extracted from all pregnant women who delivered in outpatient or inpatient of Xi' an Municipal Central Hospital. The psychological status of the pregnant women in the two groups was assessed with Hamilton Rating Scale for Depression( HRSD), Hamilton Rating Scale for Anxiety(HAMA) and Symptom Checklist 90(SCL-90). Results Incidence rates of depression and anxiety in the case group were significantly higher than the control group (X2depression = 13. 487, X2anxiety = 53. 756, both P < 0. 01 ). There were no significant differences in all physical and psychological health indexes between the two groups ( all P > 0.05 ) except hostile symptom( t = - 2.513, P < 0.05). Conclusion The psychological stress level of those pregnant women with positive HbsAg is higher than the normal pregnant women, especially the incidence rates of moderate and severe depression and moderate and severe anxiety, although their main psychic reaction and emotional response and stages are consistent to those of the normal pregnant women.%目的 分析乙肝孕产妇与正常孕产妇的心理应激的差异.方法 随机抽取69名门诊或住院就诊的HbsAg(+)孕产妇(病例组),随机抽取同期具有可比性的正常妊娠妇女123例(对照组).采用汉密尔顿抑郁量表、汉密尔顿焦虑量表及症状自评量表评定HbsAg(+)孕产妇与正常妊娠妇女的心理状况.结果 病例组抑郁和焦虑的发生率均较对照组高,经比较有显著性差异(χ2抑郁=13.487,P0.05).结论 HbsAg(+)孕产妇的心理应激水平比正常孕妇更高,以中度、重度抑郁、焦虑最为显著;其主要心理情绪反应及阶段与正常孕妇基本一致.

  3. Surveillance of risk behawiors facilitating among commercial sex works and analysis of HIV, Syphilis, HCV and HBV infection%街头暗娼危险行为监测和HIV HCV HbsAg梅毒检测结果分析

    Institute of Scientific and Technical Information of China (English)

    姜楠

    2012-01-01

    目的 分析本市女性性服务人群艾滋病病毒(HIV)、梅毒螺旋体(TP)、丙型肝炎病毒(HCV)、乙型肝炎病毒(HBV)感染情况.为我市疾病预防和控制提供依据.方法 对性服务者填写调查问卷、采集血液标本,用酶联免疫吸附试验(ELISA)、蛋白印迹试验(WB)检测、确证HIV感染;用快速血浆凝集试验(RPR)明胶颗粒凝集试验( TPPA)检测梅毒感染;用ELISA检测丙肝和乙肝.结果 最近一次与客人使用安全套的占55.69,与客人每次都用安全套的占12.66%.475例性服务人员中检出HIV抗体阳性1例(0.21%),梅毒阳性4例(0.84%),HbsAg阳性38例(11.95%),HCV阳性26例(5.42%).结论 应加大对该类人群的宣传和干预力度,充分利用各种机构促进SSWs人群的健康,从而遏制AIDS/STDR流行.%Objective To understand HIV Syphilis HCV and HBV infection among sex service crowd and to provide information for controlling these diseases. Methods Sex workers filled out questionnaires, blood collected specimens, enzyme immunosorbent adsorption test (ELISA) , protein imprinting test (WB) detection, corroborating HIV infection; With the fast plasma agglutinate experiment (RPR) gelatin particles agglutinate experiment (TPPA) testing syphilis infection ; With ELISA test hepatitis c and hepatitis b, in strict accordance with the operation of the kit. Results Guest 3. 56m Recent and guest condoms 55. 69, with the guest of every time use condoms accounted for 12. 66% . Example, sex service personnel in the check out anti -HIV positive in 1 case (accuracy is 0.21%) syphilis positive 4 cases (0. 84% ) HbsAg positive (38 cases 11. 95% ) HCV positive 26 cases (statistics show 5. 42% ). Conclusions We should strengthen the efficiency of this spread of people's health educations our chance.

  4. Incidence of anti-toxoplasma antibodies in women with high-risk pregnancy and habitual abortions

    Directory of Open Access Journals (Sweden)

    Maria de la Luz Galvan Ramirez

    1995-12-01

    Full Text Available Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, an obligate intracellular parasite. In pregnant women on the worldwide scale, there are seroprevalences from 7% to 51.3% and in women with abnormal pregnancies and abortions the seroprevalences vary from 17.5% to 52.3%. In Mexico, seropositivity has been found to vary from 18.2% to 44.8% in women with abnormal deliveries or abortions. This study's aim was to determine the incidence oflgG and IgM anti-Toxoplasma antibodies in women at the Gineco-Obstetrics Hospital of the Western Medical Center of the Mexican Social Security Institute. Three hundred and fifty women with high-risk pregnancies were studied, and 122 (34.9% were found to be IgG seropositive and 76 (20.7% were IgM positive. In one group of women with habitual abortions there were 48 (44.9% with the preseiwe of IgG antibodies and 33 (33-3% were IgM seropositive. Seropositivity was analyzed according to age, occupation, socio-economic level, eating raw or poorly cooked meat, and living with cats.La toxoplasmosis es una zoonosis causada por Toxoplasma gondii , parãsito intracellular obligado, en mujeres embarazadas a nivel mundial existen seroprevalencias del 7% al 51.3% y mujeres con embarazos anormales y aborto varian desde 17.5% al 52.3%. En México se ha referido seropositividad del 18.2% al 44.8% en mujeres con partos anormales 6 abortos. El propósito de este trabajo consistió en determinar la frecuencia de anticuerpos antitoxoplasma IgG e IgM en mujeres del Hospital de Gineco Obstetrícia del Centro Médico de Occidente del Instituto Mexicano del Seguro Social. Se estudiaron 350 mujeres con embarazo de alto riesgo encontrando 122 (34.9% seropositivas a IgG y 76 (20.7% a IgM, y en un grupo de 105 mujeres con aborto habitual resultando 48 (44.9% con presencia de anticuerpos IgG y 33 (33-3% a IgM. Se analizó la seropositividad con la edad, ocupación, nivel socioeconómicoa, ingesta de came cruda 6 mal cocida y conviveticia

  5. Seroprevalencia de hepatitis viral B en estudiantes universitarios en Abancay, Perú Seroprevalence of viral hepatitis B in university students in Abancay, Perú

    Directory of Open Access Journals (Sweden)

    Max Carlos Ramírez-Soto

    2011-09-01

    Full Text Available Para determinar la prevalencia de marcadores serológicos de hepatitis viral B en estudiantes universitarios de la ciudad de Abancay, realizamos un estudio transversal en 240 estudiantes de tres universidades, entre enero a octubre de 2010. Previo consentimiento informado, se llenó, por cada estudiante, una ficha epidemiológica y se tomó una muestra sanguínea para determinar la presencia de HBsAg, anti-HBcAg total, anti-HBe, HBeAg e IgM anti-HBc por el método de ELISA. Se encontró una prevalencia de 2,5 % (seis seropositivos para el HBsAg y 28,3 % (68 seropositivos para los anticuerpos Anti-HBcAg. El sexo masculino estuvo asociado con la presencia del anti-HBcAg (OR = 2,0; IC 95 %, 1,2- 3,6. No se encontró la presencia del HBeAg e IgM anti-HBc; los seis portadores del HBsAg fueron anti-HBe positivos. En conclusión, la infección por hepatitis B sigue siendo un problema de salud pública en Abancay, con una prevalencia importante en estudiantes universitarios.To determine the prevalence of serological markers of viral hepatitis B in university students of the city of Abancay, we performed a cross-sectional study on 240 students from three universities, from January to October 2010. Informed consent was requested to every student, an epidemiological record was filled, and a venous blood sample was drawn to determine the presence of HBsAg, total anti - HBcAg, anti - HBe, HBeAg and IgM Anti - HBc by ELISA. A prevalence of 2.5% (six positive samples was found for HBsAg and of 28.3% (68 positive samples for anti - HbcAg antibodies. The male sex was associated with the presence of anti - HBcAg (OR = 2.0, 95% CI, 1.2 to 3.6. We did not found HBeAg or IgM anti - HBc, however, the 6 HBsAg carriers were anti - HBe positive. In conclusion hepatitis B infection is still a public health problem in Abancay, with a significant prevalence in university students

  6. Simeprevir and Sofosbuvir Combination Treatment in a Patient with HCV Cirrhosis and HbS Beta 0-Thalassemia: Efficacy and Safety despite Baseline Hyperbilirubinemia

    Directory of Open Access Journals (Sweden)

    Nikolaos Papadopoulos

    2016-01-01

    Full Text Available Hyperbilirubinemia is an adverse reaction of simeprevir (SMV. The majority of these patients were taking concurrent ribavirin presenting elevated unconjugated hyperbilirubinemia due to hemolysis. However, cases of hepatic failure with elevated bilirubin level have also been reported in patients with decompensated cirrhosis. We describe a 51-year-old female patient with HbS beta 0-thalassemia and recently diagnosed compensated cirrhosis due to chronic hepatitis C infection. Laboratory evaluation revealed total bilirubin: 2.7 mg/dL and serum HCV-RNA 137.204 IU/mL. HCV was genotyped as 4. A FibroScan revealed 35.3 kPa. She was considered as illegible for pegylated-interferon-free treatment with direct acting antivirals and a course with simeprevir and sofosbuvir (SOF combination for twelve weeks was planned. Hyperbilirubinemia developed from the beginning with peak values during the 3rd month of treatment. However, no findings of liver decompensation were noticed. Hyperbilirubinemia was benign and fully reversible and our patient finally achieved sustained virological response 24 weeks after the end of treatment.

  7. Diagnosis and treatment of hepatic veno-occlusive disease induced by sedum aizoon in HBsAg positive patients%土三七致HBsAg阳性患者肝小静脉闭塞病的诊断和治疗

    Institute of Scientific and Technical Information of China (English)

    陈华忠; 董米连; 邵辉; 张智勤; 朱坚胜; 甘梅富; 阮冰

    2010-01-01

    Objective To review the diagnosis and treatment of hepatic veno-occlusive disease(HVOD)induced by sedum aizoon in HBsAg positive patients. Methods Clinical data of 35 HBsAg positive cases who took sedum aizoon decoction and developed HVOD were collected, the clinical manifestation, imaging examination, histological examination of liver puncture biopsy, and the outcomes of patients were reviewed. Results Hepatomegaly, liver dysfunction, abdominal effusion and map-like density changes in liver CT scan were observed in 35 patients. Liver biopsy wag performed in 17 patients. In histopathological examination, the swelling and point-like necrosis of liver cells, expansion and congestion of sinus, endothelial swelling, wall thickening, incomplete lumen occlusion of small liver vascular were observed. Map-like density changes in liver CT scan were found in all 17 patients who were diagnosed by histological examination. Fifteen patients presented small amount of ascites within 4 weeks of onset, 13 of whom recovered or improved after treated with low-molecular weight heparin and albumin; while among the remaining 20 patients. only half of them were benefited from the same treatments. Conclusion HVOD can be diagnosed by liver CT scan instead of histological examination; treatment of patients in early stage may improve the outcome.%目的 总结乙型肝炎表面抗原(HBsAg)阳性患者服用土三七致肝小静脉闭塞病(hepatic veno-occlusive disease,HVOD)的诊断和治疗经验.方法 对35例HBsAg阳性HVOD患者的临床表现、影像学检查和肝组织活检结果,以及治疗效果进行分析.结果 35例患者均有肝脏肿大、肝功能异常和腹腔积液等临床表现,肝脏CT有地图状密度改变.17例患者进行了肝组织活检,病理表现为肝细胞肿胀伴点状坏死;肝窦明显扩张、淤血;汇管区肝小静脉内皮肿胀,管壁增厚,管腔不完全闭塞.17例肝组织活检确诊的HVOD患者肝脏CT检查均

  8. Anti-hepatitis B Virus Activity of 8-epi-Kingiside in Jasminum officinale var. grandiflorum

    Institute of Scientific and Technical Information of China (English)

    ZHAO; Gui-qin; YIN; Zhi-feng; LIU; Li-yan; MAO; Xiao-xia; SU; Zhan-hui

    2013-01-01

    Objective To evaluate the effect of 8-epi-kingiside (8-Epik) derived from the buds of Jasminum officinale var. grandiflorum (JOG) on hepatitis B virus (HBV) replication in HepG2 2.2.15 cell line in vitro and duck hepatitis B virus (DHBV) replication in ducklings in vivo. Methods The concentration of extracellular hepatitis B e antigen and hepatitis B surface antigen (HBsAg) in cell culture medium was determined by ELISA, respectively. The anti-HBV effects of 8-Epik were also demonstrated in the model of DHBV. 8-Epik was ip given (20, 40, and 80 mg/kg, twice daily) to the DHBV-infected ducklings for 10 d. The isotonic saline liquid diet was ip given as negative control and Lamivudine (50 mg/kg, twice daily) was given as positive control. DHBV DNA was measured at days 0 (T0), 5 (T5), 10 (T10), and day 3 after cessation of treatment (P3) by dot blotting. Results 8-Epik effectively blocked HBsAg secretion in HepG2 2.2.15 cells in a dose-dependent manner [IC 50 = (19.4 ± 1.04) μg/mL]. 8-Epik (40 or 80 mg/kg, ip, twice daily) also reduced viremia in DHBV-infected ducks. Conclusion Therefore, 8-Epik is warranted as a potential therapeutic agent for HBV infection.

  9. Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L

    Directory of Open Access Journals (Sweden)

    Long Ma

    2010-10-01

    Full Text Available Suosuo grape (Vitis vinifer L is traditionally used as a therapeutic agent for measles and hepatitis by the ethnic Uighurs. This work aimed to investigate the anti-HBV effect of total triterpene (VTT, total flavonoids (VTF and total polysaccharides (VTP from Suosuo grape, and their synergistic effects were also tested. The viral antigens of cellular secretion, HBsAg and HBeAg, were determined by enzyme linked immunosorbent assay (ELISA.The quantity of HBV-DNA released in the supernatant was assayed by real-time PCR. It was found that it effectively suppressed the secretion of HBsAg and HBeAg from HepG2.2.15 cells in a dose-dependent manner, as well as the HBV DNA. The results of orthogonal design experiment showed that the combination of VTT 20 μg/mL, VTF 50 μg/mL and VTP 50 μg/mL had the best optimistic inhibitory effects on HBeAg secretion.

  10. Clinical analysis of HBsAg clearance in ten chronic hepatitis B patients treated with nucleoside analogs%核苷(酸)类似物治疗慢性乙型肝炎10例临床分析

    Institute of Scientific and Technical Information of China (English)

    许春; 李佰君; 魏倪

    2012-01-01

    本研究对10例用核苷(酸)类似物(NUCs)治疗出现HBsAg阴转或血清学转换的病例资料进行了回顾性分析,现报道如下.一、资料与方法1.病例资料:收集2005年2月至2010年8月于沈阳市第六人民医院入院的10例慢性乙型肝炎(CHB)患者应用NUCs治疗出现HBsAg阴转或血清学转换的患者资料.临床诊断符合2005年慢性乙型肝炎防治指南[11标准.

  11. Anti-hepatitis B virus activity of Boehmeria nivea leaf extracts in human HepG2.2.15 cells.

    Science.gov (United States)

    Wei, Jingchen; Lin, Lianku; Su, Xiaojian; Qin, Shaoyan; Xu, Qing; Tang, Zunian; Deng, Yan; Zhou, Yuehan; He, Songqing

    2014-01-01

    Boehmeria nivea (Linn.) Gaudich of the Urticaceae family is a perennial ratoon herbal plant, the root of which is used in traditional Chinese medicine and possesses a variety of pharmacological properties. The 20% ethanol Boehmeria nivea root extract was shown to exert an anti-hepatitis B virus (HBV) effect in vitro and in vivo; however, whether the Boehmeria nivea leaf (BNL) extract possesses similar properties has not been determined. In this study, we aimed to investigate the anti-HBV effects of the BNL extract in HepG2.2.15 cells transfected with human HBV DNA. Our results demonstrated that the secretion of HBsAg and HBeAg was reduced in HepG2.2.15 cells treated with the BNL extract, without any recorded cytotoxic effects. In addition, the chloroform fraction (CF) and ethyl acetate fraction (EAF) of BNL were shown to be more potent compared to the other fractions: CF (100 mg/l) inhibited the secretion of HBsAg by 94.00±1.78% [inhibitory concentration 50 (IC50) = 20.92 mg/l] and that of HBeAg by 100.19±0.35% (IC50=19.67 mg/l) after 9 days of treatment. Similarly, EAF (200 mg/l) inhibited the secretion of HBsAg by 89.95±2.26% (IC50=39.90 mg/l) and that of HBeAg by 98.90±1.42% (IC50=36.45 mg/l). Furthermore, we observed that the content of HBV DNA in the medium secreted by the HepG2.2.15 cells was significantly decreased under CF (100 mg/l) or EAF (200 mg/l) treatment. Thus, we concluded that the BNL extracts exhibited anti-HBV activity, with CF and EAF being the most potent among the fractions.

  12. Prevalence of HBsAg, knowledge, and vaccination practice against viral hepatitis B infection among doctors and nurses in a secondary health care facility in Lagos state, South-western Nigeria

    OpenAIRE

    Abiola, Abdul-Hakeem Olatunji; Agunbiade, Adebukola Bola; Badmos, Kabir Bolarinwa; Lesi, Adenike Olufunmilayo; Lawal, Abdulrazzaq Oluwagbemiga; Alli, Quadri Olatunji

    2016-01-01

    Introduction Hepatitis B Virus, a highly infectious blood-borne virus poses a major threat to public health globally due to its high prevalence rate and grave consequence in causing liver cirrhosis and hepatocelullar carcinoma, the third cause of cancer death worldwide. The aim is determine the prevalence of HBsAg, knowledge, and vaccination practices against viral hepatitis B infection among doctors and nurses in a health care facility. Methods Study design was a descriptive cross-sectional ...

  13. Evaluation of Colloidal Gold Immunochromatography Paper Test for HbsAg Detection%胶体金免疫层析试纸检测乙型肝炎病毒表面抗原的评价

    Institute of Scientific and Technical Information of China (English)

    沈红燕; 王万仓; 樊瑞军

    2002-01-01

    目的:对四种胶体金免疫层析测定(GICA)试纸条检测乙型肝炎病毒表面抗原(HBsAg)进行评价,以此为实验室选择提供依据.方法:采用GICA法和酶免疫(ELA)法对定值的HbsAg样品和1210份病人血清标本同时测定,测定其敏感性、特异性和物理特性.结果:四种GILA对表面抗原的最小检出量为1~5ng/ml.与ELA法比较检出率为92.7%~99.1%,未发现假阳性及前带现象.结论:GICA检测血清中HbsAg敏感性低于ELA法,成本较高,但特异性强,简便快速,无需特殊仪器设备,适用急诊和献血员的现场采血前的筛选,有一定的应用价值.

  14. After anti-racism?

    OpenAIRE

    Lentin, Alana

    2008-01-01

    Abstract Anti-racism as a political discourse and a form of collective social action has long been ignored as a serious field of research. In contrast, I envision the study of anti-racism as a vital lens on both 'race' and racism. First, the heterogeneity of anti-racism is demonstrated, spanning both pro- and anti-state-based analyses of the origins of racism. Second, a parallel discourse of 'anti-anti-racism' within the radical Left reveals the reluctance of many on the Left to id...

  15. Hallazgos de anticuerpos contra Leptospira sp., serovariedades Panama, Lai, Australis, Shermani y Patoc, en un grupo de monos rhesus (Macaca mulatta en condiciones de cautiverio Antibodies findings against Leptospira sp., of the serovars Panama, Lai, Australis, Shermani, and Patoc, in a group of rhesus monkeys (Macaca mulatta in conditions of captivity

    Directory of Open Access Journals (Sweden)

    A Ibáñez-Contreras

    2010-01-01

    Full Text Available La leptospirosis ha emergido como una importante enfermedad infecciosa, lo que ha ocurrido en diferentes entornos desde urbanos hasta medios naturales; los portadores son animales salvajes o domésticos. El objetivo fue evaluar la presencia de anticuerpos contra Leptospira en monos rhesus en condiciones de bioterio. Se utilizaron 59 monos de diferentes etapas fisiológicas. Se determinaron anticuerpos contra Leptospira a partir de la prueba de aglutinación microscópica (TAM, utilizando como antígenos 25 serovariedades de Leptospira. Se observó que de los 59 animales, únicamente el 30,50% reaccionaron contra una o más serovariedades de Leptospira. En este trabajo se determinaron anticuerpos contra cuatro serovariedades de Leptospira: Panama, Lai, Australis y Shermani, consideradas como patógenas, y once de las muestras reaccionaron contra la serovariedad Patoc, considerada no patógena y saprófita. Ninguno de los animales que resultaron positivos contra Leptospira presentó signos clínicos compatibles con leptospirosis.During the last decade leptospirosis has emerged as a worldwide and very important infectious disease, affecting different environments from urban to rural, with carriers usually being wild or domestic animals. The objective of this study was to evaluate the presence of antibodies against Leptospira in rhesus monkeys (Macaca mulatta in a Research Center. Fifty nine non-human primates of different physiological stages were studied. The animals were held in groups and in individual cages. Blood samples were obtained from the saphenous vein to determine the presence of antibodies through the microscopic agglutination test (MA, using twenty five serovars of Leptospira as antigens. It was observed that 18 out of the 59 monkeys (30.50% reacted against one or more serovars of Leptospira. In this study, antibodies of 4 serovars of Leptospira, (Panama, Lai, Australis, and Shermani were detected. All 4 serovars were considered

  16. Utilización de un anticuerpo monoclonal en el diagnóstico directo e indirecto del virus IPN en peces infectados experimentalmente Utilization of a monoclonal antibody in the diagnosis of infectious pancreatic necrosis virus in fish

    Directory of Open Access Journals (Sweden)

    F. CIFUENTES

    1999-01-01

    Full Text Available El virus de la necrosis pancreática infecciosa, virus IPN, es el agente causal de una enfermedad altamente contagiosa que afecta a peces salmonídeos jóvenes, éstos cuando sobreviven pueden convertirse en portadores que pueden transmitir la enfermedad. Para contribuir al diagnóstico rápido y efectivo del virus tanto en peces con la infección aguda, como en peces portadores, es que ensayamos la aplicación de un anticuerpo monoclonal en la detección del virus en animales infectados experimentalmente. Utilizamos alevines de truchas arcoiris (Oncorhynchus mykiss y de salmones coho (Oncorhynchus kisutch infectados con virus IPN aislado en Chile. Para detectar a los antígenos virales empleamos ensayos de ELISA inmunodot e inmunofluorescencia. Los resultados muestran que en los peces con la enfermedad aguda el virus se detecta aplicando directamente los inmunoensayos en extractos de peces enteros. En cambio, para detectar al virus en los peces portadores fue necesario incorporar un paso adicional para amplificar al virus en cultivos celulares. A pesar de lo cual, sin embargo, los métodos se optimizaron de manera que el procedimiento completo se realizó en menos de tres días para ELISA e inmunodot y menos de un día y medio para la inmunofluorescencia. Debido a que la técnica de inmunofluorescencia permite la identificación inequívoca de una o pocas células infectadas, es que ensayamos condiciones para diagnosticar al virus IPN desde improntas de peces portadores del virus. Demostramos que los antígenos virales pueden ser facilmente identificados por medio de la tinción directa de improntas de órganos de peces con el tamaño adecuadoThe infectious pancreatic necrosis virus, IPNV, is the etiological agent of a highly contagious disease that affects young salmon. If they survive, they become carriers and can transmit the disease. To contribute to the fast and effective diagnosis in fish with the acute infection, as well as of carrier

  17. Clinical significance of "anti-HBc alone" in human immunodeficiency virus-positive patients

    Institute of Scientific and Technical Information of China (English)

    Ma Teresa Pérez-Rodríguez; Bernardo Sope(n)a; Manuel Crespo; Alberto Rivera; Teresa Gonzólez del Blanco; Antonio Ocampo; César Martínez-Vázquez

    2009-01-01

    AIM: To determine the prevalence and clinical relevance of isolated antibodies to hepatitis B core antigen as the only marker of infection ("anti-HBc alone") among human immunodeficiency virus (HIV) type-1 infected patients. Occult hepatitis B infection frequency was also evaluated.METHODS: Three hundred and forty eight histories from 2388 HIV-positive patients were randomly reviewed. Patients with serological markers of hepatitis B virus (HBV) infection were classified into three groups: past hepatitis, "anti-HBc alone" and chronic hepatitis. Determination of DNA from HBV, and RNA and genotype from hepatitis C virus (HCV) were performed on "anti-HBc alone" patients.RESULTS: One hundred and eighty seven (53.7%) HIV-positive patients had markers of HBV infection: 118 past infection (63.1%), 14 chronic hepatitis (7.5%) and 55 "anti-HBc alone" (29.4%). Younger age [2.3-fold higher per every 10 years younger; 95% confidence intervals (CI) 1.33-4.00] and antibodies to HCV infection [odds ratio (OR) 2.87; 95% CI 1.10-7.48] were factors independently associated with the "anti-HBc alone" pattern. No differences in liver disease frequency were detected between both groups.Serum levels of anti-HBs were not associated with HCV infection (nor viral replication or HCV genotype), or with HIV replication or CD4 level. No "anti-HBc alone" patient tested positive for HBV DNA.CONCLUSION: "Anti-HBc alone" prevalence in HIVpositive patients was similar to previously reported data and was associated with a younger age and with antibodies to HCV infection. In clinical practice, HBV DNA determination should be performed only in those patients with clinical or analytical signs of liver injury.

  18. HBsAg阳性孕妇乙型肝炎防治知识调查及乙型肝炎疫苗联合HBIG阻断乙型肝炎病毒母婴传播的效果%Investigation of hepatitis B prevention knowledge of HBsAg positive pregnant women and effect of the hepatitis B vaccine combined with HBIG in the blocking of mother-to-fetus transmission of hepatitis B virus

    Institute of Scientific and Technical Information of China (English)

    杨少鹏; 李静

    2015-01-01

    目的:探讨乙型肝炎疫苗联合乙型肝炎免疫球蛋白(HBIG)阻断乙型肝炎病毒(HBV)母婴传播的效果。方法选择2009年1月~2011年6月本院妇产科收治的116例乙型肝炎表面抗原(HBsAg)阳性的乙型肝炎产妇以及已按照免疫程序注射过HBIG以及乙型肝炎疫苗的年龄为1岁的婴儿作为研究对象,采用自制调查问卷对产妇进行乙型肝炎防治知识调查,检测婴儿血清HBsAg。结果 HBsAg阳性产妇对于乙型肝炎能够通过母婴传播的知晓率较高,认为乙型肝炎疫苗和HBIG可以预防乙型肝炎母婴传播者所占比例为74.14%。受检婴幼儿中,HBsAg检测阳性11例(9.48%),阻断成功率为90.52%。母亲为乙肝大、小三阳所生的婴儿HBsAg阳性率分别为14.71%和2.08%,两者比较差异有统计学意义(P0.05). Conclusion To strengthen prevention and control of hepatitis B knowledge health education of preg-nant women infected with HBV,and in accordance with the immunization program on neonatal joint vaccination of hep-atitis B vaccine and HBIG,can effectively reduce the rate of mother-to-fetus transmission of hepatitis B.

  19. PRODUCCIÓN DE LA PROTEÍNA DE CHOQUE TÉRMICO HSC70 RECOMBINANTE EN Escherichia Coli BL21(DE3) PARA GENERAR ANTICUERPOS POLICLONALES PRODUCTION OF RECOMBINANT HEAT SHOCK PROTEIN HSC70 IN Escherinchia coli BL21(DE3) TO OBTAIN POLYCLONAL ANTIBODIES

    OpenAIRE

    Rocio Cely Castro; Julia Díaz Gómez; Diana Pulido; Orlando Acosta L; Carlos A Guerrero F

    2006-01-01

    Antecedentes. Los organismos vivos responden al estrés aumentando la síntesis de proteínas. El estrés por choque térmico ha sido el más estudiado y las proteínas que se inducen, se han denominado genéricamente como proteínas de choque térmico. Objetivo.En este trabajo se establecieron las condiciones óptimas de producción de la proteína HSC70, expresada en E.coli BL21 (DE3) y de los anticuerpos policlonales que permitan identificarla. Material y métodos. Mediante varios ensayos se establecier...

  20. Cross-sectional investigation on HBsAg screening and health intervention after screening among pregnant women%孕产妇乙肝表面抗原阳性筛查及筛查后健康干预现况调查

    Institute of Scientific and Technical Information of China (English)

    黄艳; 李雷雷; 孙小红; 吕梅; 刘慧慧; 唐光鹏; 王定明

    2012-01-01

    Objective: To understand the current situations of HBsAg screening among pregnant women and health intervention after screening among the pregnant women with positive HBsAg. Methods: Constant - volume sampling method was used to select eight counties from Qiandongnan Autonomous Prefecture, a hospital at county level was randomly selected from each county; 82 pregnant women with positive HBsAg who gave birth to their babies in 2010 were selected as survey objects, cross sectional investigation was carried out. Results; The screening rate of HBsAg among the pregnant women in Qiandongnan Autonomous Prefecture was 97. 68% , and the positive rate of HBsAg was 6. 14%. Among 82 pregnant women with positive HBsAg, only 54. 88% of them knew that themselves had received HBsAg screening during pregnancy, 26. 83% of them still didn't know that themselves were infected by HBV; 48.78% of them were informed to stop smoking and drinking, 47.56% of them were informed to treat on occasion, 39.02% of them were informed that the family members of the pregnant women with negative HBsAg needed to inoculate hepatitis B vaccine, 69. 52% of them were informed that hepatitis B vaccine could prevent hepatitis B. Among 82 children born by the pregnant women with positive HBsAg, only 14.63% of them received high - dose hepatitis B vaccine, 11. 76% of them received hepatitis B immunoglobulin. Only 19.51% of the children received examination after vaccination, although 98. 78% of the persons wanted to examinate the effect of hepatitis B vaccine among the children by drawing blood after vaccine. Conclusion: The screening rate of HBsAg among the pregnant women in Qiandongnan Autonomous Prefecture of Guizhou province was relatively high, but the cognition rate of the pregnant women with positive HBsAg to screening knowledge was relatively low, and the health intervention and help they got after screening were deficient. It is suggested to enhance and carry out health intervention for pregnant women

  1. Génotypes du virus de l'hépatite B et marqueurs évolutifs des patients porteurs chroniques de l'AgHBs à Bujumbura

    Science.gov (United States)

    Ntagirabiri, Rénovat; Munezero, Belyse; Nahimana, Caritas; Ndabaneze, Evariste

    2016-01-01

    Introduction L'infection par le virus de l'hépatite B (VHB) est une affection grave suite à ses complications notamment la cirrhose et le carcinome hépatocellulaire (CHC). Les génotypes du VHB influent beaucoup sur son évolution et sur l'efficacité du traitement. Le but était d’évaluer les génotypes du VHB et les profils évolutifs des patients porteurs chroniques de l'AgHBs. Méthodes Étude transversale, menée au Centre hospitalo-universitaire de Kamenge et au Centre des maladies du tube digestif et du foie « CEMADIF » entre Juin 2013 et Mai 2014. Le génotypage, les dosages quantitatifs de l'AgHBe et de l'ADN virale B ont été réalisés au Laboratoire Cerba, Cergy Pontoise, France. L’évaluation de la fibrose était faite par le Fibrotest ou le FibroScan. Résultats Au total, 143 patients, 52,4% de sexe masculin, âge moyen 38,1 ans ont été inclus. Selon les marqueurs évolutifs, 112 patients (78,3%) avaient un AgHBe négatif. Quant à la charge virale, 106 patients (74,2%) avaient une virémie inférieure à 2000UI/ml et une fibrose minime inférieure à 7kpa selon le FibroScan. Parmi eux, 13 malades avaient un ADN du VHB indétectable (0,8UI/ml). Il a été possible de déterminer le génotype chez 51 patients qui avaient une virémie assez élevée pour permettre techniquement ce dosage. Ces patients avaient tous un génotype A. Conclusion Le génotype A du VHB est le plus fréquent à Bujumbura. Il est associé à un portage inactif élevé. PMID:27222687

  2. GPC3 fused to an alpha epitope of HBsAg acts as an immune target against hepatocellular carcinoma associated with hepatitis B virus

    Institute of Scientific and Technical Information of China (English)

    Jun-Wen Yang; Dong-Ye Yang; Fang-Gen Lu; Cai-Hong Li; Hui Chen; Ning Xie; Xin Zhao

    2011-01-01

    BACKGROUND: The incidence of hepatocellular carcinoma (HCC) in China is closely related to the population infected with hepatitis B virus (HBV). HCC cells with HBV secrete soluble HBsAg into blood but do not express it on the cell membrane. This study aimed to construct and investigate a new glycosyl-phosphatidylinositol (GPI)-anchored protein (GPC3+α+EGFP) as a DNAvaccineagainstHCCassociatedwithHBV. METHODS: A recombinant plasmid (pcDNA3.1(+)/GPC3+α+EGFP) was constructed and verified by restriction endo-nuclease digestion and sequencing. pcDNA3.1(+)/GPC3+α+EGFP was transfected into HepG2 cells (experimental group) using lipofectamine 2000. pEGFP-N1-transfected HepG2 cells were used as a negative control, and non-transfected HepG2 cells sreved as a blank control. HepG2 cells that steadily expressed the fusion protein GPC3+α+EGFP were screened by G418, propagated, and co-cultured with lymphocytes from healthy donors. Cell proliferation was measured by the classic sulforhodamine B assay. Apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and Fas gene transcription was determined by quantitative fluorescent PCR. RESULTS:  The pcDNA3.1(+)/GPC3+α+EGFP plasmid was successfully constructed. In the experimental group, green fluorescence was observed at the cell periphery and in the cytoplasm, whereas in the negative control group, fluorescence was evenly distributed throughout the cell. Proliferation of the experimental group significantly decreased after 72 hours compared to the negative and blank control groups. Furthermore, the number of apoptotic cells was statistically different among the three groups as determined by a contingency table Chi-square test; the experimental group had the highest incidence of apoptosis. Fas gene transcription in the experimental group was higher than in the two control groups, and an increasing trend with time in the experimental group was observed. CONCLUSION: A chimeric

  3. HBsAg阳性的1355名孕妇乙型肝炎病毒母婴传播的随访调查%Follow-up study on hepatitis B virus mother-to-child transmission in 1355 HBsAg-positive pregnant women

    Institute of Scientific and Technical Information of China (English)

    周瑾; 尹玉竹; 吴玲玲; 张培珍; 李小毛

    2012-01-01

    Objective To investigate hepatitis B virus (HBV) mother-to-child transmission rate in hepatitis B virus surface antigen (HBsAg)-positive pregnant women.Methods A total of 1355 HBsAg-positive pregnant women and their 1360 newborns (included 5 twins) were collected prospectively.All newborns received hepatitis B immunoglobulin (HBIG) 200 U intramuscularly within 6 hours of birth as early as possible,and were administered with routine 10 μg recombinant hepatitis B vaccine (at 0,1,6 months of birth).The venous blood HBV markers and HBV DNA levels were detected in all newborns at 0,7,12 months of age.The measurement data were analyzed by t test.Qualitative data were analyzed by chi square test,rank sum test or Fisher exact test.Results The intrauterine HBV infection rate of 1360 infants were 1.54% (21/1360) during 12 months of follow-up.The rate of intrauterine infection in HBeAg positive mothers was significant higher than that of HBeAg negative mothers (4.44% vs 0,χ2 =35.99; P<0.05); the rate of intrauterine infection in HBV DNA positive mothers was significant higher than that of HBV DNA negativemothers (3.13% vs 0,χ2 =21.84; P<0.05).When maternal serum HBV DNA≥1 × 107 IU/mL,the rate of intrauterine infection was 6.01 %,which was significantly higher than that of maternal serum HBV DNA< 1 × 107 IU/mL (χ2 =39.43,P<0.05).Conclusions After strict combined active-passive immunization,the rate of HBV intrauterine infection is 1.54%.When mothers are HBeAg positive or with high level of HBV DNA,the rate of HBV intrauterine infection increases significantly.Intrauterine infection is the main cause of failure in immunoblockade of HBV mother-to-child transmission.%目的 观察HBsAg阳性孕妇所分娩婴儿的HBV感染情况.方法 前瞻性收集血清HBsAg阳性孕妇1355名及其所分娩的新生儿1360名(包括5名双胎)的资料,所有新生儿均于出生6h内注射乙型肝炎免疫球蛋白200U,并按0-1-6方案接种

  4. The immune responses induced by the DNA vaccine expressing fusion protein of HSV-2gD-HBsAg and IL-18%HSV-2gD模拟抗原表位、HBsAg与IL-18融合蛋白DNA疫苗的免疫效果观察

    Institute of Scientific and Technical Information of China (English)

    焦凤萍; 郑绮菡; 于爱莲; 王玉; 于广福

    2012-01-01

    Objective:To evaluate humoral and cellular immune responses induced by the DNA vaccine expressing fusion pro-lein of HSV-2gD-HBsAg and IL-18 in BALB/c mice. The P6 is mimolope of HSV-2gD, the NP6 is the sequence of nalural HSV-2gD which is similar Lo P6. Methods:The plasmid pcDNA3. 1 ( negative conlrol) and the recombinanl plasmids pc-S-P6-IL18 and pc-S-NP6-IL18 were inoculated into BALB/c mice by intramuscular injection for three Limes, Lwice a week. Two weeks after the last vaccination, the level of specific IgG anlibody,IFN-γ and IL-18 were measured by ELISA;one month after the last vaccination,spleen cells of vaccinated mice were separated and the proliferative response of spleen lymphocytes was stimulated by ConA and measured by MTT. Results:After inoculated by pc-S-P6-IL18 and pc-S-NP6-IL18 vaccines,the mice could produce higher level of splenocyte proliferative response and secrected IFN-γ,IL-18 and specific antibody than by pcDNA 3. 1. Conclusion:The recombinants of pc-S-P6-IL18 and pc-S-NP6-IL18 can effectively induce humoral and cellular immune responses,which can offered the basic study for preventing a-gainst the infections of hepatitis B virus and herpes simplex virus type 2.%目的:研究串联重组核酸疫苗pc(pcDNA3.1)-S(HBsAg)-P6-IL18和pc(pcDNA3.1)-S(HBsAg)-NP6-IL18对机体的免疫效果,P6是我们前期采用噬菌体展示技术筛选出的HSV-2gD的模拟抗原表位,NP6为与HSV-2gD模拟抗原表位P6最相似的天然抗原表位序列.方法:分别将空质粒pcDNA3.1(阴性对照组)和构建的真核表达质粒pc-S-P6-IL18和pc-S-NP6-IL18肌内注射免疫接种BALB/c小鼠3次,每次间隔2周.末次免疫后2周眼眶静脉采血,ELISA法检测小鼠血清特异性抗体滴度、IFN-γ及IL-18含量;末次免疫后一月,处死小鼠,无菌分离脾脏,用刀豆蛋白A刺激淋巴细胞,采用MTT法测定脾淋巴细胞增殖率.结果:重组核酸疫苗pc-S-P6-IL18和pc-S-NP6-IL18免疫小鼠后可刺激血清特异性抗体(抗HBs

  5. Evaluation of hepatic functional gain by hepato-biliary scintigraphy (HBS) after portal embolization; Evaluation du gain functionnel hepatique par la scintigraphie hepato-biliaire (SHB) apres embolisation portale

    Energy Technology Data Exchange (ETDEWEB)

    Zerbib, E.; Azoulay, D.; Wartski, M.; Castaing, D.; Bismuth, H. [Medecine Nucleaire CCML, 92350 Le Plessis-Robinson, CHB, Hopital Paul Brousse, 94804 Villejuif Cedex (France)

    1997-12-31

    The partial hepatic extirpation is an alternative therapeutics in malign tumors of liver. The post-surgery risk of hepato-cellular insufficiency (HCI) is important when the extracted volume is higher than 70 - 80% of the total volume of a non-cirrhotic liver. In this case, sometimes, a homolateral-to-tumor portal embolization (PE) was proposed in order to induce an atrophy of the embolized side and a compensating hypertrophy of the contralateral side. The HBS was proposed as a method for evaluated the functional gain after PE. Six patients have benefited by 2 HBS: one of them before PE, the others, 3 weeks after. The dynamic acquisition was achieved after intravenous injection of 300 MBq of {sup 99m}Tc - tBIDA, on a DST (SMV) camera equipped with HRBE collimators. The regions of interest (ROI) corresponding to right and left liver, were drawn on an anterior and posterior image of the `parenchymatous` time (the forth or fifth minute), allowing the calculation of the functional ratio by arithmetic mean. The gain of the functional ratio of healthy liver was in average of 7% (ranging from 1 to 13%). These results appear to be comparable with those from scanner in 5 cases but different in one case (functional gain without volumetric gain at scanner); this last patient was finally successfully operated. Totally, the surgery indication was posed in these 6 patients, one of whom, solely, on arguments of scintigraphic quantification. The HBS allows estimating the variations in functional ratios before and after PE. This is a supplementary tool in the evaluation of the feasibility of a major hepatectomy after PE

  6. Anti-Hepatitis B Virus Activity of Chickweed [Stellaria media (L. Vill.] Extracts in HepG2.2.15 Cells

    Directory of Open Access Journals (Sweden)

    Donghao Xie

    2012-07-01

    Full Text Available Stellaria media (Linn. Villars is a traditional Chinese medicine that has been used for over 200 years, mainly for the treatment of dermatitis and other skin diseases. It has also been used as an anti-viral agent. All the fresh chickweed juice samples used in this study were prepared using macroporous resin and ultrafiltration technology. The anti-hepatitis B virus (HBV activity of S. media was evaluated in vitro using the human HBV-transfected liver cell line HepG2.2.15. The concentrations of hepatitis B surface antigen (HBsAg and hepatitis B e antigen (HBeAg in HepG2.2.15 cell culture medium were determined by enzyme-linked immunosorbent assay (ELISA after S. media-n (SM-n treatment for 6 or 9 days. HBV DNA was quantified using transcription-mediated amplification and real-time polymerase chain reaction. In HepG2.2.15 cells, 30 μg/mL SM-3 effectively suppressed the secretion of HBsAg and HBeAg with inhibition rates of 27.92% and 25.35% after 6 days of treatment, respectively. Consistent with the reduction in HBV antigens, SM-3 also reduced the level of HBV DNA in a dose-dependent manner. The characterization and quantitation of the chemical composition of SM-3 showed the presence of flavonoid C-glycosides, polysaccharides, and protein, which exhibited diverse antiviral activities. In conclusion, our results demonstrate that SM-3 possesses potential anti-HBV activity in vitro. This is the first report demonstrating the anti-HBV effects of S. media, which is currently under early development as a potential anti-HBV drug candidate.

  7. Analysis of HBsAg/NAT test results in Shenzhen city in 2003-2012%深圳地区2003~2012年间无偿献血者抗-HCV 抗体与核酸检出情况分析∗

    Institute of Scientific and Technical Information of China (English)

    熊姣梅; 吴桂丹; 郑欣; 杨爱莲; 魏天莉

    2015-01-01

    目的:调查分析深圳地区2003~2013年献血者抗-丙型肝炎病毒(HCV)抗体/病毒核酸检测(NAT)阳性检出情况。方法献血者经乙型肝炎病毒表面抗原(HBsAg)、丙氨酸氨基转移酶(ALT)快速试纸条初筛后,使用进口与国产两种酶联免疫吸附试验(ELISA)及血液病毒 NAT 方法检测抗-HCV 抗体、HCV RNA,对11年来献血者抗-HCV 抗体、NAT 阳性率流行趋势进行统计分析比较。结果深圳地区2003~2013年献血人数逐年增长,抗-HCV 抗体阳性率呈 M 型检出走势,于2013年达到最低。献血者抗-HCV 抗体阳性/NAT 阴性检出率与抗-HCV 抗体走势一致,而真实感染 HCV 的指标抗-HCV 抗体与 NAT 双阳性组检出率则缓慢下降;对抗-HCV 抗体阳性献血者在不同分类组别比较发现,31~45岁献血者组、初次献血者组的抗-HCV 抗体检出率分别高于其他年龄组与重复献血者(P <0.05);抗-HCV 抗体阴性/NAT 阳性献血者有3例(检出率为1/134518)。结论NAT 检测为常规酶联免疫方法的补充检测手段,缺一不可,能大大降低输血风险,而 NAT 阴性献血者的抗-HCV 抗体阳性占多数的检测结果应引起重视,避免因假阳性导致此类献血者资格淘汰。%Objective To investigate and analyze the popular trend of HBV infection in blood donors in Shenzhen City from 2003 to 2012.Methods After preliminary routine strip screening of HBsAg、ALT,blood samples were tested by using both domestic and imported ELISA reagents along with nucleic acid detection,then tried to find a trend of HCV prevalence in blood donor by analysis data.Results Blood donors′number rised year by year from 2003 to 2013,but prevalence of anti-HCV gave a volatile reception, which had a lowest rate at 2013.The detection rate of anti-HCV positive/NAT-was basically the same as anti-HCV positive,where-as the detection rate of anti-HCV positive/NAT positive declined slightly in those confirmed HCV infectors;blood donors were

  8. Risk of Severe Acute Exacerbation of Chronic HBV Infection Cancer Patients Who Underwent Chemotherapy and Did Not Receive Anti-Viral Prophylaxis.

    Directory of Open Access Journals (Sweden)

    Chih-An Shih

    Full Text Available Reactivation of HBV replication with an increase in serum HBV DNA and alanine aminotransferase (ALT activity has been reported in 20-50% of hepatitis B carriers undergoing cytotoxic chemotherapy for cancer treatment. Manifestation of HBV reactivation ranges from asymptomatic self-limiting hepatitis to severe progressive hepatic failure and fatal consequences.To investigate the risk of severe acute exacerbation of chronic HBV infection in HBsAg-positive cancer patients with solid tumors or hematological malignancies who underwent chemotherapy without antiviral prophylaxis.A retrospective review of charts was conducted for HBsAg-positive cancer patients in our institution who underwent chemotherapy and did not receive anti-viral prophylaxis between the periods of July 2007 to January 2013. We investigate the incidence of severe acute exacerbation of chronic HBV infection if these patients with a variety of solid tumors and hematological malignancies.A total of 156 patients (hematological malignancies: 16; solid tumors: 140 were included. The incidence of severe acute HBV exacerbation in the patients with hematological malignancy was higher than that in solid tumors (25.0% [4/16] vs 4.3% [6/140]; P = 0.005. Additionally, patients receiving rituximab-based chemotherapy had higher acute exacerbation rate than those with non-rituximab-based chemotherapy (40.0% vs 4.1%, P = 0.001. Among the patients with solid tumors, the incidences of severe acute exacerbation of chronic HBV in hepatocellular carcinoma, colorectal cancer, lung cancer, breast cancer, gynecological cancer, urological tract cancer, head/neck cancer and other solid malignancies were 2.3%, 4.0%, 7.1%, 9.0%, 16.7%, 6.7%, 0% and 0%, respectively.Severe acute exacerbation of chronic HBV infection may occur in HBsAg-positive patients with a variety of solid tumors who received chemotherapy without adequate anti-viral prophylaxis. Hematological malignancy and rituximab-based chemotherapy are

  9. Níveis de vale de ciclosporina elevados em transplantados renais anti-HCV positivos Elevated cyclosporine A trough levels in HCV positive kidney transplant recipients

    Directory of Open Access Journals (Sweden)

    Luciano Wolffenbüttel

    2003-06-01

    Full Text Available OBJETIVO: Comparar os níveis de vale de CsA de transplantados renais anti-HCV+ com um grupo controle. MÉTODOS: Incluímos como casos todos os pacientes anti-HCV+ transplantados entre janeiro de 1992 e abril de 1996, e os anti-HCV- transplantados a seguir do caso como controles. Excluímos pacientes diabéticos, HbsAg+, os que recebiam fármacos com interação com a CsA e aqueles com transaminases elevadas. A sorologia para HCV foi testada pelo método ELISA de 3ª geração, e as dosagens de ciclosporina através de fluorimetria polarizada com anticorpo policlonal. RESULTADOS: As principais variáveis demográficas não diferiram entre os grupos. O nível de vale médio de CsA do primeiro mês pós-transplante foi maior nos 23 pacientes anti-HCV+ (551 ± 280 ng/ml do que nos 31 controles (418 ± 228 ng/ml, pOBJECTIVE: Compare the CsA trough levels of HCV+ kidney transplant recipients to a control group METHODS: All anti-HCV positive patients that received a renal allograft between January 1992 and April 1996 were initially included as cases. Patients with diabetes mellitus, HBsAg+, who were taking medication that could modify CsA pharmacokinetics and those with elevated aminotransferases were excluded. For each anti-HCV positive index case the following transplanted anti-HCV negative patient was included as a control. Third generation ELISA was used for determination of the anti-HCV status and CsA dosages were performed by polarized fluorometry with polyclonal antibodies. RESULTS: No differences in the demographic variables were found. The average CsA through levels in the first month were higher (551 ± 280 ng/ml in the 23 cases as compared to the 31 controls (418 ± 228 ng/ml; p< 0.05. The differences became apparent at the end of the first week (528 ± 275 versus 344 ± 283 ng/ml; p<0.01 and persisted at discharge (582 ± 284 versus 457 ± 229; p=0,08. CONCLUSION: We concluded that anti-HCV positive patients have higher blood levels of Cs

  10. Adjuvant CpG ODN increases production of anti-HBs induced by HBV gene vaccines%佐剂CpG ODN对HBV基因疫苗诱导抗体产生的影响

    Institute of Scientific and Technical Information of China (English)

    李文波; 姚志强; 周永兴; 冯志华

    2000-01-01

    目的探讨人工合成的CpG ODN作为佐剂对乙肝病毒基因疫苗诱导小鼠产生抗HBs的影响. 方法构建编码乙型肝炎病毒(HBV)表面抗原蛋白S的重组真核表达质粒pCR3.1-S作为HBV基因疫苗, 人工合成含CpG motif的硫代磷酸寡核苷酸作为佐剂, 以BALB/c小鼠作为实验动物进行免疫接种, 采用ELISA法检测免疫小鼠的抗HBs应答. 结果 与生理盐水作为佐剂组相比较,应用CpG ODN组小鼠产生更强的抗HBs反应(P<0.05). 结论 CpG ODN作为佐剂可促进HBV基因疫苗诱导BALB/c小鼠产生抗HBs应答.

  11. Anti-HBs levels in infants of hepatitis B carrier mothers after delayed active immunization with recombinant vaccine concomitant with DTP-polio vaccine: Is there need for a second dose of HBIg?

    NARCIS (Netherlands)

    P.M. Grosheide (Pia Maria); R. Del Canho (R.); M. Voogd (M.); R.A. Heijtink; S.W. Schalm (Solko)

    1994-01-01

    textabstractThe need for an additional dose of hepatitis B immune globulin (HBIg) was studied by comparing infants receiving 1 ml HBIg at birth followed by hepatitis B immunization, concomitant with DTP-polio vaccine, at 3, 4, 5 and 11 months (schedule E), with infants receiving the same schedule wi

  12. Study of quantitative assay and calculate method with RIA A dot-assay in titrating IU of anti-HBs%抗-HBs国际单位RIA一点法定量测定与计算方法的探讨

    Institute of Scientific and Technical Information of China (English)

    席三强; 虞现河

    2001-01-01

    在Radio-immuno Assay(RIA)试验测定抗-HBs国际单位的简易定量与计算中,采用RIA一点法测定,以分析表达式mIU/ml=SC(mIU/ml)[exp(0.69315S-NC/SC-NC)-1]进行结果计算.此式可以常用对数式表示为mIU/ml=SC(mIU/ml)[lg-1(0.30103S-NC/SC-NC)-1],当(S-NC)/(SC-NC)在0.3~1.1界线内时,其计算结果的相对误差小于6%,是目前误差最小的简易计算方法.同时还推算求出Holliger公式的阳性对照的最适含量为124.26mIU/ml,Richardson公式的阳性对照为125 mIU/ml,中国药品生物制品检定所公式的阳性对照为92.5 mIU/ml.

  13. PRODUCCIÓN DE LA PROTEÍNA DE CHOQUE TÉRMICO HSC70 RECOMBINANTE EN Escherichia Coli BL21(DE3 PARA GENERAR ANTICUERPOS POLICLONALES PRODUCTION OF RECOMBINANT HEAT SHOCK PROTEIN HSC70 IN Escherinchia coli BL21(DE3 TO OBTAIN POLYCLONAL ANTIBODIES

    Directory of Open Access Journals (Sweden)

    Rocio Cely Castro

    2006-07-01

    Full Text Available Antecedentes. Los organismos vivos responden al estrés aumentando la síntesis de proteínas. El estrés por choque térmico ha sido el más estudiado y las proteínas que se inducen, se han denominado genéricamente como proteínas de choque térmico. Objetivo.En este trabajo se establecieron las condiciones óptimas de producción de la proteína HSC70, expresada en E.coli BL21 (DE3 y de los anticuerpos policlonales que permitan identificarla. Material y métodos. Mediante varios ensayos se establecieron las concentraciones óptimas del agente inductor isopropil- -D-thiogalacto­piranosido (PTG, del inóculo bacteriano, de empleo de los plásmidos de expresión pET-3a y pET-28a (+; y el método más eficiente para la recuperación de las formas soluble o insoluble de la proteína y de anticuerpos policlonales que la identifiquen. Resultados. Encontramos que el inóculo de cinco colonias con IPTG (2mM en tubos con cinco mililitros de medio modificado e incubadas por 24 horas a 37°C con agitación constante (200 r.p.m y tratamiento de sonicación, produce el mejor rendimiento de HSC70. La calidad de la proteína inducida se estableció mediante “Western blotting”. Conclusión. La proteína recombinante así obtenida permitió, generar anticuerpos policlonales que a su vez permiten detectar la proteína HSC70 natural en la membrana citoplasmática de diferentes células por inmunofluorescencia, en ELISA, en Western Blot y en pruebas de bloqueo de infección de rotavirus.Background. Live organisms respond against stressing conditions raising the production of stress proteins. The heat shock stress is a very well kwon condition that produces heat shock proteins. Objective. In this work we determined the optimum conditions to produce HSC70 protein in E. coli BL12 (DE3 under stress conditions, and the best way to obtain its polyclonal antibodies. Materials and methods. The best concen­trations for isopropyl- ß -D

  14. Anti-addiction vaccines

    Science.gov (United States)

    Shen, Xiaoyun; Orson, Frank M.

    2011-01-01

    Despite intensive efforts to eradicate it, addiction to both legal and illicit drugs continues to be a major worldwide medical and social problem. Anti-addiction vaccines can produce the antibodies to block the effects of these drugs on the brain, and have great potential to ameliorate the morbidity and mortality associated with illicit drug intoxications. This review provides a current overview of anti-addiction vaccines that are under clinical trial and pre-clinical research evaluation. It also outlines the development challenges, ethical concerns, and likely future intervention for anti-addiction vaccines. PMID:22003367

  15. Encefalitis aguda mediada por anticuerpos contra el receptor ionotrópico de glutamato activado por N-metil-D-aspartato (NMDAR: análisis de once casos pediátricos en Argentina (Premio Benito Yelín

    Directory of Open Access Journals (Sweden)

    Elisa Pérez

    2013-09-01

    Full Text Available Las encefalitis son procesos inflamatorios de diverso origen, entre las cuales se incluyen las autoinmunes. La identificación de anticuerpos contra el receptor N-metil-D-aspartato permitió la caracterización clínico-inmunológica de una entidad susceptible a tratamiento inmunomodulador. Descripta originalmente en mujeres jóvenes, asociada a teratoma de ovario, es hoy una entidad reconocida en niños aun en ausencia de tumores detectables. El objetivo del trabajo, realizado a través de la revisión de historias clínicas, fue describir las características clínicas, evolutivas y los hallazgos en estudios complementarios de once niños con diagnóstico confirmado de esta entidad a través de la identificación de los anticuerpos específicos. Todos debutaron con síntomas psiquiátricos, en nueve de ellos asociando a convulsiones, y dos a movimientos extrapiramidales. En su evolución, todos presentaron compromiso del lenguaje, nueve, síntomas autonómicos graves, en uno con hipoventilación y requerimientos de ARM. La RM de cerebro fue anormal en tres. Ocho tuvieron EEG con asimetría del voltaje y/o amplitud, tres de ellos presentaron espigas. En el LCR seis tuvieron pleocitosis y tres de siete bandas oligoclonales positivas. Cinco, serologías IgM para micoplasma. El aumento de CPK en coincidencia con antipsicóticos ocurrió en cinco. Con el tratamiento inmunomodulador, cinco tuvieron recuperación total, tres, alteraciones conductuales/cognitivas y uno déficits severo. Un paciente resolvió su cuadro clínico sin tratamiento. En ninguno se detectó tumor asociado. Concluimos que frente a un niño con encefalopatía aguda y clínica compatible con esta entidad, una vez descartada causa infecciosa, se debe comenzar tratamiento inmunomodulador precozmente, evitar la utilización de fármacos antipsicóticos y realizar la búsqueda de posibles tumores ocultos.

  16. SITUASI PATEN OBAT ANTI DIABETES, ANTI HIPERTENSI, ANTI MALARIA DAN ANTI TUBERKULOSIS DI INDONESIA

    OpenAIRE

    Basundari Sri Utami; Sekar Tuti; Anggita Bunga Anggraini; Mukhlissul Faatih; Siswanto Siswanto; Trihono Trihono

    2014-01-01

    AbstrakIndonesia merupakan negara berpenduduk keempat terbanyak setelah Cina, India dan Amerika. Indonesia sedang mengalami transisi epidemiologi, dimana terjadi peningkatan penyakit tidak menular (PTM), sementara penyakit menular (PM) seperti malaria, tuberkulosis dan demam dengue prevalensinya masih tinggi. Tingginya morbiditas merupakan lahan yang bagus untuk melaksanakan obat anti PM dan anti PTM yang mendapat paten karena pangsa pasarnya yang sangat luas. Sayangnya potensi pasar yang mas...

  17. Anti-addiction vaccines

    OpenAIRE

    Shen, Xiaoyun; Orson, Frank M.; Kosten, Thomas R.

    2011-01-01

    Despite intensive efforts to eradicate it, addiction to both legal and illicit drugs continues to be a major worldwide medical and social problem. Anti-addiction vaccines can produce the antibodies to block the effects of these drugs on the brain, and have great potential to ameliorate the morbidity and mortality associated with illicit drug intoxications. This review provides a current overview of anti-addiction vaccines that are under clinical trial and pre-clinical research evaluation. It ...

  18. Efetividade das vacinas anti-VHB (DNA-recombinante em doadores de sangue de uma região endêmica para hepatite B no sul do Brasil Effectiveness of recombinant DNA vaccines against hepatitis B in blood donors in an endemic region of South Brazil

    Directory of Open Access Journals (Sweden)

    Andrea Petry

    2006-10-01

    Full Text Available O objetivo deste estudo foi de estimar a efetividade das vacinas anti-VHB em um estudo longitudinal, retrospectivo composto por 1.012 doadores de sangue que completaram o esquema padrão de vacinação (três doses, incluindo doses de reforço nos doadores com títulos de anti-HBs The objective of this work was to estimate the effectiveness of DNA recombinant anti-HBV vaccines in a retrospective cohort study of 1,012 Brazilian blood donors who completed the vaccination schedule (3 doses + booster of antibody titer <10IU/L during the period 1998-2002. The results showed that seroconversion rates were significantly lower among the donors whose antibody titers was measured six months after completing the vaccination scheme and among older donors, particularly those aged over 50. Overall vaccine effectiveness was 88.7%, ranging from 80.6% in the oldest (50 years or over to 91.4% among the youngest (18-30 years donors. The booster regimen was effective at reducing the percentage of non-responders. We conclude that vaccine effectiveness was significantly better in younger blood donors and that the anti-HBs testing interval influenced the vaccine effectiveness.

  19. Detection of anti-preS1 antibodies for recovery of hepatitis B patients by immunoassay

    Institute of Scientific and Technical Information of China (English)

    Jun Wei; Yu-Qin Wang; Zhi-Meng Lu; Guang-Di Li; Yuan Wang; Zu-Chuan Zhang

    2002-01-01

    AIM: To establish a convenient immuncassay method based on recombinant antigen preS1(21-1l9aa) to detect anti-preS1antibodies and evaluate the clinical significance of antibodies in hepatitis B.METHODS: The expression plasmid pET-28a-preS1 wasconstructed, and a large quantity of preS1 (21-119aa)fragment of the large HBsAg protein was obtained. ThepreS1 fragment purified by Ni2+ -IDA affinity chromatographywas used as coated antigen to establish the indirect ELISAbased on streptavidin-biotin system for detection of the anti-preS1 antibodies in sera from HBV-infected patients. Forfollow-up study, serial sera were collected during theclinical course of 21 HBV-infected patients and anti-preS1antibodies, preS1 antigen, HBV-DNA and other serologicalHBV markers were analyzed.RESULTS: preS1 (21-119aa) fragment was highly expressedfrom the plasmid pET-28a-preS1 in a soluble form in E. Coli(30 rog@ L-1 ), and easily purified to high purity over 90 % byone step of Ni2+ -IDA-sepharose 6B affinity chromatography.The purity and antigenicity of the purified preS1 (21-119aa)protein was determined by 150 g@ L-1 SDS-PAGE, Westemblot and a direct ELISA. Recombinant preS1 (21-119aa)protein was successfully applied in the immunoessay whichcould sensitively detect the anti-preS1 antibodies in serumspecimens of acute or chronic hepatitis B patients. Resultsshowed that more than half of 19 acute hepatitis B patientsproduced anti-preS1 antibodies during recovery of thedisease, however, the response was only found in a few ofchronic patients. In the clinical follow-up study of 11patients with anti-preS1 positive serological profile, HBsAgand HBV-DNA clearance occurred in 6 of 10 acute hepatitis Bpatients in 5-6 months, and seroconversion of HBeAg anddisappearance of HBV-DNA occurred in 1 chronic patientstreated with lavumidine, a antiviral agent.CONCLUSION: The high-purity preS1 ( 21-119aa) coatedantigen was successfully prepared by gene expression andaffinity chromatography. Using this

  20. Diagnostic incidence of the presence of positive HBsAg: epidemiologic, clinical, and virological characteristics Incidencia diagnóstica de AgHBs positivo: Características epidemiológicas, clínicas y virológicas

    Directory of Open Access Journals (Sweden)

    Elvira Poves Martínez

    2012-01-01

    Full Text Available Objective: to analyze the epidemiological, clinical, and virological characteristics of patients newly diagnosed with active hepatitis B virus (HBV infection based on the presence of positive hepatitis B surface antigen (HBsAg in the digestive diseases department of a district hospital. Patients and methods: we performed a 3-year prospective study in patients newly diagnosed with HBV infection. We analyzed epidemiological, clinical, and virological characteristics, complete HBV markers, quantification of HBV DNA, and infection by hepatitis delta virus. We performed genotyping and resistance testing in patients with a high viral load. Results were obtained for patients who required liver biopsy. Results: we diagnosed 213 patients (18.8/10,000 inhabitants/year. Men accounted for 61%, and 59% were aged 20 to 40 years. Immigrants accounted for 53% of the population: 46% were from Rumania and 37% from Sub-Saharan African countries. At diagnosis, 2.3% had acute hepatitis (all with jaundice and 3.3% had cirrhosis with portal hypertension. With the exception of cases of acute hepatitis, positive HBeAg was observed in 9%. Serum transaminase levels were normal in 62.2% of patients, HBV DNA was > 2,000 IU/mL in 33.8%, and delta virus was present in 3.3%. Genotyping and resistance testing were performed in 70 patients: the most common genotype was D, followed by A. Resistance was detected at baseline in only 2 cases: to adefovir in one case and to entecavir in another. Among the 36 biopsies performed, 32.4% showed inflammatory activity ≥ 2, and 23.5% had fibrosis ≥ 2 according to the METAVIR scoring system. According to clinical practice, specific treatment for HBV infection was necessary (any reason in 17.4% of those diagnosed (3 patients per 100,000 inhabitants/year. Conclusions: despite prevention and vaccination, HBV infection is a health problem that most commonly affects the immigrant population and men. Serum transaminase levels are normal in 62

  1. Anti-nutritional Factors.

    Science.gov (United States)

    2016-01-01

    Anti-nutritional factors such as trypsin inhibitor, phytic acid and cyanogen are as important as nutritional content of any edible plant part. The anti-nutritional factors can be defined as those substances generated in natural food substances by the normal metabolism of species and by different mechanisms (e.g. inactivation of some nutrients, diminution of the digestive process or metabolic utilization of feed) which exert effects contrary to optimum nutrition. Hence, trypsin inhibitor, phytic acid and cyanogens present in edibles with the methods in the chapter would be helpful. PMID:26939264

  2. Two distinct subtypes of hepatitis B virus-related acute liver failure are separable by quantitative serum immunoglobulin M anti-hepatitis B core antibody and hepatitis B virus DNA levels

    DEFF Research Database (Denmark)

    Dao, Doan Y; Hynan, Linda S; Yuan, He-Jun;

    2012-01-01

    Hepatitis B virus (HBV)-related acute liver failure (HBV-ALF) may occur after acute HBV infection (AHBV-ALF) or during an exacerbation of chronic HBV infection (CHBV-ALF). Clinical differentiation of the two is often difficult if a previous history of HBV is not available. Quantitative measurements...... of immunoglobulin M (IgM) anti-hepatitis B core antibody (anti-HBc) titers and of HBV viral loads (VLs) might allow the separation of AHBV-ALF from CHBV-ALF. Of 1,602 patients with ALF, 60 met clinical criteria for AHBV-ALF and 27 for CHBV-ALF. Sera were available on 47 and 23 patients, respectively. A quantitative......) of the AHBV-ALF group had no hepatitis B surface antigen (HBsAg) detectable on admission to study, wheras no CHBV-ALF patients experienced HBsAg clearance. Rates of transplant-free survival were 33% (20 of 60) for AHBV-ALF versus 11% (3 of 27) for CHBV-ALF (P = 0.030). CONCLUSIONS: AHBV-ALF and CHBV...

  3. The Anti-Landscape

    DEFF Research Database (Denmark)

    humanities. The Anti-Landscape provides an interdisciplinary approach that moves beyond the false duality of nature vs. culture, and beyond diagnosis and complaint to the recuperation of damaged sites into our complex heritage. Collection of essays based on a conference held at SDU in 2011....

  4. Cloning and expression of truncated HBcAg gene,whole-length HBcAg gene and HBc-HBsAg fusion gene in vitro%截短序列和全序列HBcAg基因以及HBV C-S融合基因的克隆与表达

    Institute of Scientific and Technical Information of China (English)

    朱翔; 路文明; 丁宁玲; 叶建中; 王锋; 沙莉; 李扬; 高胜兰

    2015-01-01

    目的:构建截短序列和全序列HBcAg基因和HBc-HBsAg融合基因原核表达质粒,研究目的蛋白在大肠杆菌中的表达及其免疫原性。方法利用HBV全基因(adr亚型)质粒pUCmT-HBV分别扩增HBsAg截短基因、HBcAg截短基因和HBcAg全基因,构建成重组质粒pSK-HBs、pSK-HBc和pKS-HBV C,经DNA序列测定鉴定后,分别将HBcAg截短基因、HBcAg全基因及HBc-HBsAg融合基因亚克隆至表达质粒PET-30a,在大肠杆菌BL21(DE3)中进行表达HBcAg截短基因、HBcAg全基因和HBc-HBsAg融合基因产物,采用PAGE-SDS和免疫印迹法对表达产物进行鉴定。结果成功构建了含HBcAg截短基因、HBcAg全基因和HBc-HBsAg融合基因的原核表达质粒;成功构建的质粒在大肠杆菌BL21(DE3)中能大量表达HBcAg蛋白和HBc-HBsAg融合蛋白,免疫印迹分析结果显示表达产物具有免疫原性。结论成功构建的原核表达载体在大肠杆菌BL21(DE3)中能顺利表达HBcAg蛋白和HBc-HBsAg融合蛋白,表达产物具有免疫原性,为慢性乙型肝炎特异性免疫治疗研究提供了实验基础。%Objective To construct the prokaryotic recombinant plasmids carring truncated HBcAg gene, whole-length HBcAg gene and HBc-HBsAg fusion gene,and to observe the expression of target proteins in E.coli and their immunogenicity in vitro. Methods Truncated HBcAg gene,whole-length HBcAg gene and HBc-HBsAg fusion gene were obtained from plasmid pUCmT-HBV containing whole-length HBV gene (subtype adr)and con-struct recombinant plasmids of pSK-HBs,pSK-HBc and pKS-HBV C. Truncated HBcAg gene,whole-length HBcAg gene and HBc-HBsAg fusion gene which were obtained by fusing truncated HBsAg and truncated HBcAg gene,were subcloned into a expression vector pET-30a respectively after confirmed by DNA sequencing. The gene products were expressed in E.coli BL21 (DE3) and identified by SDS-PAGE and Western blot. Results The prokaryotic expression plasmids

  5. Anti- CC-Groups and Anti-PC-Groups

    Directory of Open Access Journals (Sweden)

    Francesco Russo

    2007-01-01

    subgroup H of G. An anti-PC group G is a group in which each nonfinitely generated subgroup K has the quotient group G/coreG(NG(K which is a polycyclic-by-finite group. Anti-CC groups and anti-PC groups are the subject of the present article.

  6. SITUASI PATEN OBAT ANTI DIABETES, ANTI HIPERTENSI, ANTI MALARIA DAN ANTI TUBERKULOSIS DI INDONESIA

    Directory of Open Access Journals (Sweden)

    Basundari Sri Utami

    2014-10-01

    Full Text Available AbstrakIndonesia merupakan negara berpenduduk keempat terbanyak setelah Cina, India dan Amerika. Indonesia sedang mengalami transisi epidemiologi, dimana terjadi peningkatan penyakit tidak menular (PTM, sementara penyakit menular (PM seperti malaria, tuberkulosis dan demam dengue prevalensinya masih tinggi. Tingginya morbiditas merupakan lahan yang bagus untuk melaksanakan obat anti PM dan anti PTM yang mendapat paten karena pangsa pasarnya yang sangat luas. Sayangnya potensi pasar yang masih luas ini hanya ditangkap oleh luar negeri. Data dari Direktorat Jendral Hak Kekayaan Intelektual (Ditjen HKI pada tahun 2010 menunjukkan pemohon paten dalam negeri yang mendapatkan persetujuan perlindungan paten (granted hanya 4,6% sedangkan dari luar negeri sebanyak 92,03%. Hal yang sangat ironis bagi Indonesia yang merupakan negara dengan potensi bahan dasar obat alam dan keanekaragaman hayati terbanyak ketiga setelah Brazil dan Cina. Tujuan penelitian ini untuk mengevaluasi situasi paten obat yang terdaftar di Direktorat Paten, Ditjen HKI, Kementerian Hukum dan Hak Asasi Manusia RI dalam 7 tahun terakhir (tahun 2005 sampai 2011 untuk PM (malaria dan tuberkulosis dan PTM (hipertensi dan diabetes. Metode observasional dengan penelusuran dokumen paten dari alamat web instansi terkait. Hasilnya Indonesia hanya mendaftarkan 4,9% dari seluruh paten yang didaftarkan di Dirjen HKI dari tahun 2005 sampai dengan 2011, sebagai berikut untuk obat anti-hipertensi 3,4% dari 89 paten, anti-diabetes hanya 4,8% dari 250 paten, anti malaria 21,1% dari 18 paten anti-tuberkulosis 7,1% dari 14 paten. Sebagian besar paten yang didaftarkan oleh pendaftar Indonesia merupakan paten obat ekstrak herbal atau komposisinya. Kesimpulan dari penelitian ini adalah paten obat untuk PTM dan PM di Indonesia masih didominasi paten luar negeri.Kata Kunci : Situasi paten, obat, Ditjen HKI, IndonesiaAbstractIndonesia is the fourth most populous country after China, India and America. There

  7. Anti-Microtubule Drugs.

    Science.gov (United States)

    Florian, Stefan; Mitchison, Timothy J

    2016-01-01

    Small molecule drugs that target microtubules (MTs), many of them natural products, have long been important tools in the MT field. Indeed, tubulin (Tb) was discovered, in part, as the protein binding partner of colchicine. Several anti-MT drug classes also have important medical uses, notably colchicine, which is used to treat gout, familial Mediterranean fever (FMF), and pericarditis, and the vinca alkaloids and taxanes, which are used to treat cancer. Anti-MT drugs have in common that they bind specifically to Tb in the dimer, MT or some other form. However, their effects on polymerization dynamics and on the human body differ markedly. Here we briefly review the most-studied molecules, and comment on their uses in basic research and medicine. Our focus is on practical applications of different anti-MT drugs in the laboratory, and key points that users should be aware of when designing experiments. We also touch on interesting unsolved problems, particularly in the area of medical applications. In our opinion, the mechanism by which any MT drug cures or treats any disease is still unsolved, despite decades of research. Solving this problem for particular drug-disease combinations might open new uses for old drugs, or provide insights into novel routes for treatment. PMID:27193863

  8. Evaluación de la seroprotección contra sarampión, rubéola y hepatitis B en niños menores de cinco años del Perú, 2011 Evaluation of the seroprotection against measles, rubella and hepatitis B in children under 5 years of age in Peru, 2011

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    Víctor Fiestas Solórzano

    2012-12-01

    Full Text Available Objetivos. Estimar la prevalencia de anticuerpos contra sarampión, rubéola y hepatitis B en niños de 1 a 4 años del Perú. Materiales y métodos. Se realizó una encuesta nacional basada en la aplicación de un cuestionario y obtención de muestra de sangre capilar en papel de filtro para el estudio de anticuerpos contra sarampión, rubéola y hepatitis B en niños de 1 a 4 años. Se utilizó un muestreo probabilístico, estratificado y multietápico con inferencia a nivel nacional y siete ámbitos de estudio: Lima metropolitana, resto de costa urbana, costa rural, sierra urbana, sierra rural, selva urbana y selva rural. Las muestras de sangre capilar fueron procesadas siguiendo protocolos estandarizados para la determinación de anticuerpos mediante técnica de ELISA utilizando reactivos comerciales. Resultados. Se encontró una prevalencia nacional de 91,6% (IC95%: 90,6-92,7%, 91,3% (IC 95%: 90,3-92,4% y 95,9% (IC 95%: 95,0-96,8% para anticuerpos contra sarampión, rubéola y hepatitis B respectivamente. No se evidenció diferencias significativas de las prevalencias entre los diferentes ámbitos de estudio y en los diferentes estratos socioeconómicos de los conglomerados. Conclusiones. En niños de 1 a 4 años se ha estimado una prevalencia nacional de anticuerpos contra sarampión y rubéola entre 90-93%, mientras que para anticuerpos contra hepatitis B (anti-HBsAg entre 95-97%.Objectives. To estimate the prevalence of antibodies against measles, rubella and hepatitis B in children aged between 1 and 4 years in Peru. Materials and methods. A national survey was conducted based on a questionnaire and capillary blood sample taken on filter paper in order to study antibodies against measles, rubella and hepatitis B in children from 1 to 4 years of age. A stratified, multistage, probability sampling design was used to be representative at the national level and at level of seven ambits, including the Metropolitan Lima Area, the rest of the

  9. ELISA cualitativo de IgA anti-Lipopolisacárido de Vibrio cholerae en saliva de humanos

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    Judith Mónica del Campo

    2002-03-01

    Full Text Available Se estandarizó un ELISA para detectar el principal antígeno inductor de protección de Vibrio cholerae en saliva IgA contra el lipopolisacárido (LPS. El estudio se llevó a cabo en voluntarios que fueron inoculados por vía oral con dosis de 0, 107, 108, 109 unidades formadoras de colonias (ufc del candidato vacunal El Tor Ogawa, cepa 638. Las muestras de saliva fueron tomadas de forma seriada, a los 0, 7, 8, 9, 10 y 14 días postinoculación. Se consideró seroconversión si las densidades ópticas eran superiores al nivel de corte y si los incrementos después de inmunizar duplicaban los valores antes de la inmunización. Los resultados, al ser comparados con los grupos experimentales, condición individuos inoculados y los placebos, demostraron que la técnica tiene una sensibilidad del 93,3%, una especificidad del 96,0%, un valor predictivo positivo de 98,2% y negativo de 85,7%, y una eficiencia del 94,1%. Se demostró la presencia de IgA anti LPS en saliva de los individuos inoculados con el candidato vacunal, con una mayor concentración de anticuerpos con el inóculo de (109 ufc y se obtuvo la máxima positividad a los nueve días.

  10. Hepatitis B or hepatitis C co-infection in individuals infected with human immunodeficiency virus and effect of anti-tuberculosis drugs on liver function

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    Padmapriyadarsini C

    2006-01-01

    Full Text Available Background: Tuberculosis (TB and hepatitis are the two common co-infections in patients infected with human immunodeficiency virus (HIV. Anti-tuberculosis treatment (ATT may have an effect on the liver enzymes in these co-infected HIV patients. Aims: To determine the prevalence of Hepatitis B and C virus coinfection in HIV infected patients in Tamilnadu and assess effects of anti-tuberculosis drugs on their liver function. Settings: HIV positive subjects referred to the Tuberculosis Research Centre, Chennai Materials and Methods: All HIV infected patients referred to the Tuberculosis Research centre, from March 2000 to May 2004, were screened for Hepatitis B surface antigen (HBsAg & Hepatitis C virus (HCV antibodies by enzyme linked immunoabsorbent assay (ELISA. HIV infection was confirmed using two rapid tests and one ELISA. Patients were given either short- course anti-tuberculosis treatment or preventive therapy for tuberculosis, depending on the presence or absence of active TB, if their baseline liver functions were within normal limits. None of these patients were on antiretroviral therapy during the study period. Statistical Analysis: Paired t-test was used to find the significance between baseline and end of treatment liver enzymes levels, while logistic regression was done for assessing various associations. Results: Of the 951 HIV-infected patients, 61 patients (6.4% were HBsAg positive, 20 (2.1% had demonstrable anti HCV antibodies in their blood. Serial estimation of liver enzymes in 140 HIV patients (81 being co-infected with either HBV or HCV showed that 95% did not develop any liver toxicity while they were on anti-tuberculosis treatment or prophylaxis. Conclusions: The prevalence of hepatitis B and C coinfection was fairly high in this largely heterosexually infected population supporting the use of more careful screening for these viruses in HIV positive persons in this region. Anti-tuberculosis therapy as well as TB preventive

  11. Prescription Nonsteroidal Anti-Inflammatory Medicines

    Science.gov (United States)

    MENU Return to Web version Prescription Nonsteroidal Anti-Inflammatory Medicines Prescription Nonsteroidal Anti-Inflammatory Medicines How do prescription nonsteroidal anti-inflammatory drugs work? Nonsteroidal anti-inflammatory drugs (also called NSAIDs) stop cyclooxygenase ...

  12. Effectiveness of DNA-recombinant anti-hepatitis B vaccines in blood donors: a cohort study

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    Petry Andrea

    2007-11-01

    Full Text Available Abstract Background Although various studies have demonstrated efficacy of DNA-recombinant anti-hepatitis B vaccines, their effectiveness in health care settings has not been researched adequately. This gap is particularly visible for blood donors, a group of significant importance in the reduction of transfusion-transmitted hepatitis B. Methods This is a double cohort study of 1411 repeat blood donors during the period 1998–2002, involving a vaccinated and an unvaccinated cohort, with matching of the two in terms of sex, age and residence. Average follow-up was 3.17 person-years. The outcome measure was infection with hepatitis B virus (HBV, defined by testing positive on serologic markers HBsAg or anti-HBC. All blood donors were from the blood bank in Joaçaba, federal state of Santa Catarina, Brazil. Results The cohorts did not differ significantly regarding sex, age and marital status but the vaccinated cohort had higher mean number of blood donations and higher proportion of those residing in the county capital Joaçaba. Hepatitis B incidences per 1000 person-years were zero among vaccinated and 2,33 among non-vaccinated, resulting in 100% vaccine effectiveness with 95% confidence interval from 30,1% to 100%. The number of vaccinated persons necessary to avoid one HBV infection in blood donors was estimated at 429 with 95% confidence interval from 217 to 21422. Conclusion The results showed very high effectiveness of DNA-recombinant anti-HBV vaccines in blood donors. Its considerable variation in this study is likely due to the limited follow-up and the influence of confounding factors normally balanced out in efficacy clinical trials.

  13. Anti-hepatitis B virus activities of cinobufacini and its active components bufalin and cinobufagin in HepG2.2.15 cells.

    Science.gov (United States)

    Cui, Xiaoyan; Inagaki, Yoshinori; Xu, Huanli; Wang, Dongliang; Qi, Fanghua; Kokudo, Norihiro; Fang, Dingzhi; Tang, Wei

    2010-01-01

    Cinobufacini (Huachansu) is a Chinese medicine prepared from the skin of Bufo bufo gargarizans Cantor (Bufonidae), which has long been used in traditional Chinese medicine (TCM). The aim of present study was to examine the anti-hepatitis B virus (HBV) activities of cinobufacini and its active components bufalin and cinobufagin in the human HBV-transfected cell line HepG2.2.15. The hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and hepatitis B core-related antigen (HBcrAg) concentrations in cell culture medium were determined by chemiluminescent enzyme immunoassay after HepG2.2.15 cells were respectively treated with different concentrations of cinobufacini, bufalin, and cinobufagin for 3 or 6 d. HBV DNA and mRNA were determined using transcription-mediated amplification and real-time polymerase chain reaction (PCR), respectively. On d 3, cinobufacini at a concentration of 1 µg/ml had no activity against HBV virological markers. However, on d 6, cinobufacini at 1 µg/ml effectively inhibited the secretion of HBsAg, HBeAg, and HBcrAg by 29.58, 32.87, and 42.52%. It was more potent than the positive control lamivudine (100 µg/ml). Bufalin and cinobufagin slightly inhibited HBV antigen secretion. Treatment with cinobufacini, bufalin, or cinobufagin had no anti-HBV effect on DNA in cell culture medium. Consistent with the HBV antigen reduction, HBV mRNA expression was markedly inhibited in comparison to the control when HepG2.2.15 cells were treated with cinobufacini, bufalin, or cinobufagin. Results suggested that cinobufacini had more potent activity against HBV antigen secretion than its components bufalin and cinobufagin and this inhibitory role was attributed to the specific inhibition of HBV mRNA expression.

  14. Anti-inflammatory Activity.

    Science.gov (United States)

    2016-01-01

    Inflammation is the body's first response to infection or injury and is critical for both innate and adaptive immunity. It can be considered as part of the complex biological response of vascular tissues to harmful stimuli such as pathogens, damaged cells, or irritants. The search for natural compounds and phytoconstituents that are able to interfere with these mechanisms by preventing a prolonged inflammation could be useful for human health. Here, the anti-inflammatory properties of plant-based drugs are put together with both in vitro and acute (carrageenan, egg albumin and croton oil) and chronic (cotton pellet) in vivo models. PMID:26939273

  15. Anti-Sharing

    OpenAIRE

    Kirstein, Roland; Cooter, Robert

    2005-01-01

    The paper proposes a mechanism that may implement first-best effort in simultaneous teams. Within the framework of this mechanism, each team members is obliged to make a fixed, non-contingent payment, and chooses his individual effort. After the output is produced, each team member receives a gross payment that equals the actual team output. We demonstrate that a Nash equilibrium exists in which each team member chooses first-best effort. We call this mechanism ?Anti-Sharing? since it solves ...

  16. Immunogenicity of Hepavax-Gene recombinant hepatitis B vaccine in INML workers, Colombia.

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    Heber Siachoque

    2009-11-01

    Full Text Available Objetivo: Evaluar la inmunogenicidad de la vacuna recombinante Hepavax-Gene para hepatitis B desde la última dosis administrada en trabajadores del INML. Materiales y métodos: Estudio de corte transversal en 603 trabajadores de la salud con mínimo 3 dosis de vacuna recombinante (0, 1, 6 meses donde se midieron los niveles de anticuerpos anti-HBs con la técnica de ELISA entre diciembre de 2000 y enero de 2001 desde la aplicación de la última dosis de la vacuna, que varió entre 1 y 6 años. Resultados: El grupo de estudio lo conformaron 344 hombres y 259 mujeres, con un promedio de edad de 38.8±7.3 años. El nivel de protección fue 90.7% (>10 U/l que disminuyó significativamente con el tiempo de aplicación de la última dosis de la vacuna (p45 (RRI=3.58, IC 95%:1.83, 6.99 con respecto a Conclusión: La vacuna recombinante Hepavax-Gene anti-HBs tiene alta efectividad en los trabajadores de la salud (90.7% aunque presenta disminución de protección a mayor tiempo de aplicación de la última dosis y al aumentar la edad del trabajador.

  17. Asociación entre el estado de la función tiroidea, parámetros metabólicos, niveles de anticuerpos anti tiroperoxidasa y los niveles séricos de la Adipocitoquina chemerin / Association Between the state of thyroid function, metabolic parameters, thyroid peroxidase antibody levels and levels of serum adipocytokine chemerin

    OpenAIRE

    López Pompey, Néstor Alfonso

    2012-01-01

    Chemerin es una adipocitoquina que se expresa principalmente en tejido adiposo, descrita recientemente, la cual ha sido asociada con el desarrollo de obesidad y síndrome metabólico, es codificada por el gen RARRES2. Se ha sugerido que las alteraciones metabólicas asociadas con la disfunción tiroidea podrían en parte atribuirse a cambios en expresión y secreción de adipocitoquinas tales como resistina, leptina, adiponectina y visfatina en el tejido adiposo. No existen a la fecha informes qu...

  18. Seroprevalence of anti-HCV and hepatitis B surface antigen in HIV infected patients

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    Tankhiwale S

    2003-01-01

    Full Text Available Human immunodeficiency virus (HIV is known to influence the natural history of infections with certain hepatitis viruses and interactions between HIV and hepatitis viruses may potentiate HIV replication. There is high degree of epidemiological similarity between hepatitis B virus and HIV as regard to high-risk group and route of transmission. Transmission of hepatitis C virus (HCV through blood transfusion and intravenous drug abuse is well documented. Present study deals with the study of concurrent infection of HBV and HCV with HIV infection. In the study of 110 HIV seropositive patients, 34(30.4% were positive for HBV and 8(7.27% for HCV. The difference of concomitant infection was highly significant compared to controls. (p value < 0.0001. Heterosexual high risk behaviour was observed in 89(80.91% of 110 HIV positive patients, out of which 23(25.8% and 5(5.62% were HBsAg and anti-HCV positive respectively. History of transmission was unclear in remaining patients. Concomitant infection of HIV and HBV was found to be significantly more in the symptomatic group (40.68% compared to asymptomatic group (19.6%. As HIV infection is known to affect the natural history of both HBV and HCV infection, screening of their concurrent association is necessary.

  19. Study on in vitro inhibiting effect of Rabdosia Serra in anti-HBV%溪黄草抗乙型肝炎病毒体外抑制作用研究

    Institute of Scientific and Technical Information of China (English)

    庞琼; 胡志立

    2016-01-01

    Objective To provide an important scientific basis for verifying the in vitro anti-HBV activity of single herb Rabdosia Serra. Methods 50%ethanol extract of Rabdosia Serra was dispensed as certain concentrations. The HepG2.2.15 cel-lular model was adopted to conduct the cellular toxicity test and HBsAg and HBeAg enzyme-linked immunosorbent ssay(ELISA). Results IC50 was 4.275 mg/mL,IC50 of crude extract of Rabdosia Serra on HBsAg was 2.250 mg/mL,which on HBeAg was 2.150 mg/mL,the therapeutic index(TI)<2. Conclusion The crude extract of Rabdosia Serra has the effect for in vitro inhibiting HBV.%目的:为证实溪黄草单味药具有体外抗乙型肝炎病毒活性提供重要的科学依据。方法将溪黄草50%乙醇提取物配置成一定药物浓度,拟采用HepG2.2.15细胞模型进行细胞毒试验和乙型肝炎表面抗原(HBsAg)、乙型肝炎e抗原(HBeAg)酶联免疫检测。结果半数细胞抑制时其浓度为4.275 mg/mL,溪黄草粗提物对HBsAg的半抑制浓度(IC50)为2.250 mg/mL,对HBeAg的IC50为2.150 mg/mL,治疗指数小于2。结论溪黄草粗提物具有抑制体外乙型肝炎病毒的作用。

  20. Anti-cartilage antibody.

    Science.gov (United States)

    Greenbury, C L; Skingle, J

    1979-08-01

    Antibody to cartilage has been demonstrated by indirect immunofluorescence on rat trachea in the serum of about 3% of 1126 patients with rheumatoid arthritis. Titres ranged from 1:20 to 1:640. The antibody was not found in 284 patients with primary or secondary osteoarthritis or in 1825 blood donors, nor, with the exception of two weak reactors, in 1314 paraplegic patients. In most cases the antibody appears to be specific for native type II collagen. Using this as an antigen in a haemagglutination test 94% of anti-cartilage sera were positive, whereas among 100 rheumatoid control sera there were only three weak positives. More than 80% of patients with antibody had some erosion of articular cartilage, but there was no correlation with age, sex, duration of disease, nor any recognisable clinical event or change.

  1. [Anti-NMDA receptor encephalitis: two paediatric cases].

    Science.gov (United States)

    González-Toro, M Cristina; Jadraque-Rodríguez, Rocío; Sempere-Pérez, Ángela; Martínez-Pastor, Pedro; Jover-Cerdá, Jenaro; Gómez-Gosálvez, Francisco

    2013-12-01

    Introduccion. La encefalitis asociada a anticuerpos antirreceptores de N-metil-D-aspartato (NMDA) es una patologia neurologica autoinmune documentada en la poblacion pediatrica de manera creciente en los ultimos años. Se presentan dos casos de nuestra experiencia con clinica similar. Casos clinicos. Caso 1: niña de 5 años que inicia un cuadro de convulsiones y alteracion de conciencia, asociando trastornos del movimiento y regresion de habilidades previamente adquiridas que evoluciona a autismo. Caso 2: niña de 13 años que presenta hemiparesia izquierda, movimientos anomalos, trastorno de conducta y disautonomia. En ambos casos se obtienen anticuerpos antirreceptores de NMDA positivos en el liquido cefalorraquideo y se diagnostican de encefalitis antirreceptor de NMDA. En el primer caso se inicia el tratamiento con perfusion intravenosa de corticoides e inmunoglobulinas y es necesario asociar rituximab. En el segundo, corticoides e inmunoglobulinas. La evolucion fue favorable en ambas pacientes, con una leve alteracion del lenguaje como secuela en el primer caso y una recaida en el segundo caso, con resolucion completa. Conclusion. La encefalitis antirreceptor de NMDA es un trastorno tratable y es importante el diagnostico y tratamiento precoz, ya que mejora el pronostico y disminuye las recaidas.

  2. Prevalencia de anticuerpos contra virus dengue en el cantón de Golfito (2005 y en el Distrito Central de Puntarenas (2005-2006, Costa Rica Dengue seroprevalence in Golfito and the central district of Puntarenas, Costa Rica 2005-2006

    Directory of Open Access Journals (Sweden)

    Margarita Lee-Lui

    2008-09-01

    Full Text Available Justificación y objetivo: La primo infección con el virus dengue varía desde asintomática hasta cuadros muy severos, como el dengue hemorrágico o el síndrome de choque por dengue. El Distrito Primero de Puntarenas ha sido una de las poblaciones más afectadas desde 1993, cuando aparecieron los primeros brotes, hasta hoy, con una disminución de la incidencia en los últimos años. La región Brunca fue la segunda en incidencia en el país en 1998, y si bien la endemicidad se ha mantenido, no existen estudios epidemiológicos sobre la prevalencia de esta enfermedad; los datos que aporta el Ministerio de Salud corresponden a la incidencia de casos clínicos en un período determinado. El objetivo de este estudio fue determinar la seroprevalencia en el Distrito Primero de Puntarenas y en Golfito para conocer la vulnerabilidad de la población de sufrir fiebre hemorrágica por dengue y analizar la situación asociada a los datos epidemiológicos de estas regiones, tales como edad, sexo y ubicación geográfica, ya que una población susceptible es la que presenta una alta seroprevalencia, unida a condiciones de hacinamiento y alta densidad vectorial. Métodos: El muestreo se realizó con el método EPI descrito por la OMS. Se recolectaron 210 muestras de suero en cada distrito, se desarrolló y estandarizó una prueba de ELISA de captura de antígeno para la detección de anticuerpos tipo IgG contra dengue, usando como referencia el método de reducción de placas de Dulbecco considerado estándar de oro. Resultados: La seroprevalencia contra dengue fue del 43.8% (92 y del 90% (189 en los distritos de Golfito y Central de Puntarenas, respectivamente, sin diferencias significativas respecto al sexo, la edad y la localidad. Se obtuvo un 83% y un 95% de correlación en Golfito y Puntarenas, respectivamente, entre los casos sintomáticos y la serología con el método estandarizado. Un 32% y un 42% del total de seropositivos en cada localidad

  3. Pesquisa de anticuerpos seroneutralizantes para pestivirus y herpesvirus en ovinos, caprinos y camélidos sudamericanos de Chile Survey for antibodies to pestivirus and herpesvirus in sheep, goats, alpacas (Lama pacos, llamas (Lama glama guanacos (Lama guanicoe and vicuña (Vicugna vicugna from Chile

    Directory of Open Access Journals (Sweden)

    M Celedón

    2001-01-01

    Full Text Available Se investigó la presencia de anticuerpos por dilución punto final seroneutralizante (DPFSN para pestivirus (cepa NADL del virus de la diarrea viral bovina y para herpesvirus (cepa Los Angeles del virus herpes bovino 1, en muestras de sueros de 321 ovinos, 322 caprinos, 74 alpacas (Lama pacos , 43 llamas (Lama glama , 48 guanacos (Lama guanicoe y 34 vicuñas (Vicugna vicugna , procedentes de diferentes regiones del país. Para pestivirus se detectó reacción serológica positiva en 60 (18,7% ovinos, en 21 (6,5% caprinos, en 8 (10,8% alpacas y en 6 (14% llamas. Los guanacos y vicuñas fueron seronegativos para pestivirus. Para herpesvirus, la seropositividad se obtuvo en 8 (2,5% ovinos y 62 (19,3% caprinos. No se detectaron anticuerpos para herpesvirus en las muestras de camélidos. Según la distribución geográfica los mayores porcentajes de positividad resultaron ser: para pestivirus en ovinos de 2/3 predios de la XII Región (con positividad de 66,7% y 82,1%, con rangos de títulos de 16 a 710 y, para herpesvirus en caprinos de 5/6 predios de la IV Región (con positividad de 4,2%, 13,3%, 28,6%, 61,5% y 66,7%, con rango de títulos de 2 a 45. Las alpacas y llamas serorreaccionantes a pestivirus se encontraban ubicadas en la Región Metropolitana, en confinamiento en conjunto con otras especies de rumiantes, en cambio que las especies silvestres, guanacos y vicuñas fueron muestreadas en sus lugares de origen. Se confirma que en Chile existe infección por pestivirus en ovinos, caprinos, llamas y alpacas y por herpesvirus en ovinos y caprinosMicrotitration serum virus-neutralization tests were used to determine antibody titres for pestivirus: bovine viral diarrhea virus (NADL strain and herpesvirus: bovine herpes virus 1 (Los Angeles strain in 321 sheep, 322 goats, 74 alpacas (Lama pacos , 43 llamas (Lama glama , 48 guanacos (Lama guanicoe and 34 vicuñas (Vicugna vicugna , from several Regions of Chile. Antibodies to pestivirus were found

  4. Anti-androgen treatments.

    Science.gov (United States)

    Bachelot, Anne; Chabbert-Buffet, Nathalie; Salenave, Sylvie; Kerlan, Véronique; Galand-Portier, Marie-Béatrice

    2010-02-01

    1. Estrogen plus progestin contraceptives (EPP) are the first-line treatment of moderate hirsutism and acne in women of child bearing age (grade C). 2. CPA, 50mg/day, 20 days out of 28, associated with estrogen is the first-line treatment of "moderate to severe hirsutism" in women of childbearing age (grade C). 3. Spironolactone, given as a contraceptive, can be proposed as a second-line treatment in case of side effects or counter-indications to CPA in moderate to severe hirsutism (grade C) in women of childbearing age. No market authorization in this indication. 4. Flutamide or Finasteride are "only" to be used under the guise of contraception as a "thirdline therapy" in cases of severe hirsutism, the presence of side effects or counter-indications to EPP, CPA 50mg/day or spironolactone (grade C). No market authorization in this indication 5. There is no indication for GnRH analogs as an anti-androgen treatment in women of childbearing age given the current therapeutic alternatives (grade C) 6. Only long-term hair removal treatments can be proposed (grade C): electrolysis or laser hair removal. PMID:20096826

  5. Anti-parallel triplexes

    DEFF Research Database (Denmark)

    Kosbar, Tamer R.; Sofan, Mamdouh A.; Waly, Mohamed A.;

    2015-01-01

    -parallel TFO strand was modified with Y with one or two insertions at the end of the TFO strand, the thermal stability was increased 1.2 °C and 3 °C at pH 7.2, respectively, whereas one insertion in the middle of the TFO strand decreased the thermal stability 1.4 °C compared to the wild type oligonucleotide......-1-yl chain, especially at the end of the TFO strand. On the other hand, the thermal stability of the anti-parallel triplex was dramatically decreased when the TFO strand was modified with the LNA monomer analog Z in the middle of the TFO strand (ΔTm = -9.1 °C). Also the thermal stability...... decreased about 6.1 °C when the TFO strand was modified with Z and the Watson-Crick strand with adenine-LNA (AL). The molecular modeling results showed that, in case of nucleobases Y and Z a hydrogen bond (1.69 and 1.72 Å, respectively) was formed between the protonated 3-aminopropyn-1-yl chain...

  6. The anti pp yields anti λ λ reaction near threshold

    International Nuclear Information System (INIS)

    Measurements of differential and integrated cross sections as well as final state polarizations for the anti p p → anti Λ Λ reaction are presented. The reaction was studied at two incident antiproton momenta (1476.5 MeV/c and 1507.5 MeV/c) corresponding to total center of mass energies of 15.5 MeV and 26.4 MeV above the reaction threshold. The trajectories of charged decay products of the anti Λ and Λ were observed in a multiwire proportional chamber and in two sets of drift chambers. The data were analyzed with a computer program which reconstructed anti p p → anti Λ Λ → anti p π+pπ- events and performed kinematic fitting. The results are compared to several recent meson exchange calculations, and a one-gluon exchange calculation. The experiment was performed at the Low Energy Antiproton Ring (LEAR) at CERN. The data presented represent the first results of the PS185 collaboration's study of the threshold production of hyperon-antihyperon states

  7. Seroprevalencia de los virus de la Hepatitis A y B en grupos etarios de Costa Rica

    Directory of Open Access Journals (Sweden)

    Mayra L. Taylor

    2001-10-01

    Full Text Available Justificación y Objetivos: Debido a la persistencia de casos y brotes de hepatitis viral A y B en el país, junto con la observación de un aumento importante de infecciones por el tipo A en la población de adultos, se planteó la necesidad de establecer la prevalencia de estos dos tipos de hepatitis en diferentes grupos etarios en nuestro país. Métodos: La seroprevalencia de los virus de la hepatitis A (VHA y de hepatitis (VHB; fueron investigadas en 873 muestras de niños pre-escolares menores de 7 años de la encuesta nacional de nutrición en 1996; 614 niños escolares de 9 a 15 años de una encuesta de nutrición de la Universidad de Costa Rica realizada en 1997 y 996 muestras en adultos, estudiantes y funcionario de esta misma universidad recolectadas en 1994. Las muestras se analizaron por anticuerpos totales contra al VHA (anti-VHA total, VHB antígeno de superficie (HbsAG y anticuerpos totales contra la proteína del núcleo o "core" (anti-HBc total. Resultados: Se encontró un 6.8%, 13.2% y 71.7% de anti-VHA total en los prescolares, escolares y adultos, respectivamente, con una creciente prevalencia en relación con la edad. La presencia de estos anticuerpos en los escolares estuvo asociada con la escolaridad de los padres y su condición socieconómica; siendo mayor cuando los padres tenían un menor nivel de escolaridad (24% con secundaria incompleta versus 7.6% con nivel educativo superior y entre padres sin capacitación técnica o profesionales (16% versus 8.7% en los padres con estudios superiores. También se encontró una diferencia en la prevalencia de anti-VHA entre estudiantes de escuelas públicas (19.5% versus privadas (6.9%. En los preescolares, el único grupo con representación estadística geográfica; la provincia de Guanacaste presentó un riesgo de 2 a 3 veces mayor de tener anticuerpos contra el VHA (21% que los niños procedentes de otras provincias, lo cual es concordante con los datos relacionados con

  8. Study of anti-deuterons, anti-tritons and anti-{sup 3}He at Belle

    Energy Technology Data Exchange (ETDEWEB)

    Kuenze, Stephanie; Galuska, Martin; Gessler, Thomas; Kuehn, Wolfgang; Lange, Jens Soeren; Muenchow, David; Semmler, Diego; Spruck, Bjoern; Ullrich, Matthias; Werner, Marcel [2. Physikalisches Institut, Universitaet Giessen (Germany)

    2010-07-01

    Events of the type e{sup +}e{sup -}{yields} anti d+X, anti t+X, anti-{sup 3}He+X, are rare events in e{sup +}e{sup -} collisions as they require the production of several anti-quarks together with their corresponding quarks (6+6 in the deuteron case, 9+9 for triton and {sup 3}He). In addition, in order to form a bound state, the anti-quarks must be close in phasespace, so that coalescence (i.e. overlap of the wave functions) can be applied. Preliminary results from Belle at {radical}(s){approx_equal}10.6 GeV with a data set of 589fb{sup -1}, using dE/dx in the Belle drift chamber for particle identification, are presented. The momentum distribution of the anti-nuclei can be compared to a fireball model. Preliminary results of the search for anti-{sup 4}He are also presented.

  9. HBV父婴垂直传播水平与HBV-DNA载量的相关研究%A correlation analysis between the rate of vertical transmission of HBV and HBsAg-positive father to infant and the rate of neonatal cord blood HBV-DNA

    Institute of Scientific and Technical Information of China (English)

    张荣莲; 罗颖; 谢婧娴; 陈起燕; 成玲; 郭胜斌; 黄欣欣

    2010-01-01

    Objective To study the influence of HBV-DNA with different load levels of HBsAg-positive among fathers on the rate of neonatal cord blood HBV-DNA.Methods Using HBsAg and HBV-DNA as screening indicators for pregnant women and their husbands from an obstetric clinic.161 pregnant women whose HBsAg and HBV-DNA were negative,but HBsAg was positive among their husbands and their newborns,were selected.Blood samples from those pregnant women,their husbands and their newborns were collected to detect the related indicators.Using ELISA to detect hepatitis B virus markers(HBVM),and FQ-PCR to detect the levels of HBV-DNA load.According to neonatal cord blood HBV-DNA detection guideline,newborns with cord blood HBV-DNA positive were selected as cases,others as controls.Results(1)Result of the study showed that there was a dose-response relationship between paternal serum HBV-DNA load levels and neonatal cord blood HBV-DNA positive rates in newborns(trend χ~2=64.117,P=0.000).The rate of vertical transmission of HBV from HBsAg-positive father to infant in the paternal serum HBV-DNA>1.0×107 copies/ml group was significantly higher than HBV-DNA<1.0×107 copies/ml group(χ~2=71.539,P=0.000).(2)There was a positive rank correlation between semen positive HBeAg and vertical transmission of HBV from HBsAg-positive father to infant(χ~2=6.892,P=0.009).Conclusion There was a dose-response relationship between paternal serum HBV-DNA load levels and neonatal cord blood HBV-DNA positive in newborns.Paternal serum HBV-DNA≥1.0×107 copies/ml and with HBeAg positive status were risk factors of vertical transmission of HBV from HBsAg-positive father to infant.%目的 探讨HBsAg阳性父亲血HBV-DNA的不同载量水平对其新生儿发生HBV父婴垂直传播的影响.方法 对161例HBsAg阳性的父亲及其新生儿(母亲血清HBVM全阴性或仅HBsAb阳性及HBV-DNA均为阴性)HBV感染状况进行调查分析.采用ELISA检测HBVM,FQ-PCR法检测血清HBV DNA

  10. Thyroid autoantibodies in autoimmune diseases Anticuerpos antitiroideos en enfermedades autoinmunes

    OpenAIRE

    Regina M. Innocencio; João H. Romaldini; Ward, Laura S.

    2004-01-01

    Abnormalities in the thyroid function and thyroid autoantibodies have been frequently described in patients with autoimmune diseases but seldom in antiphospholipid syndrome patients. In order to determine the prevalence of thyroid function and autoimmune abnormalities, we compared serum thyrotropin (TSH, serum free thyroxine (T4) levels, thyroid antithyroglobulin (TgAb) and antithyroperoxidase (TPOAb) levels of 25 patients with systemic sclerosis, 25 patients with rheumatoid arthritis and 13 ...

  11. Neue Anti-Spam-Techniken

    OpenAIRE

    Richter, Frank

    2004-01-01

    Workshop "Netz- und Service-Infrastrukturen" Dieser Beitrag zum Workshop "Netz- und Service-Infrastrukturen" 2004 analysiert den Stand der Anti-Spam-Maßnahmen an der TU Chemnitz und zeigt neue Techniken auf.

  12. Online Anti-Brand Herds

    DEFF Research Database (Denmark)

    Langley, David J.; Tan, Chee-Wee; Worm, Daniël;

    The online environment offers a fertile breeding ground for anti-brand herds of disgruntled consumers. Firms are often caught off guard by the unpredictability of such herds and, as a consequence, are forced into a reactive, defensive stance. We conduct a social media analysis that aims to shed...... light on the formation, growth, and dissolution of online anti-brand herds. First we expand on the concept of environmental turbulence to advance core properties unique to online herd behavior. Next, based on evidence gathered from 40 online anti-brand herd episodes targeting two prominent firms from...... the Netherlands, we develop an analytical model to investigate drivers of herd formation, growth, and dissolution. Finally, combining environmental turbulence literature with our empirical findings, we derive a novel typology of online anti-brand herd behaviors, and put forward six propositions to guide theory...

  13. Anti-reflux surgery - children

    Science.gov (United States)

    ... stomach). Problems with these muscles can lead to gastroesophageal reflux disease (GERD). This surgery can also be done during a ... Anti-reflux surgery is usually done to treat GERD in children only after medicines have not worked ...

  14. [Reversible cortical atrophy secondary to anti-NMDA receptor antibody encephalitis].

    Science.gov (United States)

    Bravo-Oro, Antonio; Acosta-Yebra, Danae; Grimaldo-Zapata, Ilse P; Reyes-Vaca, Guillermo

    2015-05-16

    Introduccion. La encefalitis por anticuerpos antirreceptor de N-metil-D-aspartato (NMDA) inicialmente se describio como un sindrome paraneoplasico asociado a teratoma de ovario, pero cada vez con mas frecuencia se han ido publicando casos en mujeres jovenes y niños como un cuadro encefalopatico autoinmune secundario en el 40-50% de los casos a un proceso viral. Clinicamente, se caracteriza por un cuadro progresivo de manifestaciones psiquiatricas, crisis convulsivas, discinesias y disautonomias. Un hallazgo neurorradiologico poco comunicado es la atrofia cortical reversible, de la cual se desconoce su mecanismo. Caso clinico. Niña que a los 6 años comenzo con crisis convulsivas focales, con electroencefalograma epileptogeno y tomografia de craneo inicial normal. Se inicio tratamiento anticonvulsionante. A las tres semanas aparecieron nuevas crisis convulsivas, manifestaciones psiquiatricas y alteraciones en el ciclo de sueño-vigilia. Ante la sospecha de encefalitis por anticuerpos antirreceptor de NMDA, estos se determinaron en el suero y el liquido cefalorraquideo con resultado positivo. Resonancia magnetica durante el ingreso con atrofia cortical generalizada. Oncologia Pediatrica descarto asociacion a tumores. A los dos años del cuadro, con la paciente libre de crisis convulsivas, una valoracion neuropsicologica mostro la afectacion de funciones ejecutivas y una resonancia magnetica de control evidencio la recuperacion de la atrofia cortical. Conclusion. El mecanismo de la atrofia cortical reversible se desconoce, pero en pacientes con encefalitis por anticuerpos antirreceptor de NMDA podria ser directamente proporcional a la cantidad de anticuerpos circulantes y el tiempo de exposicion a estos en la corteza cerebral. Es muy importante el diagnostico temprano y el inicio de inmunomodulacion.

  15. Anti Rh Hemolytic Disease due to Anti C Antibody: Is Testing for Anti D Antibodies Enough?

    OpenAIRE

    Negi, Gita; Singh, Gaur Dushyant

    2011-01-01

    Rh blood group system is a complex blood group system. Rh antibodies are produced in Rh negative individuals following exposure to foreign RBCs after transfusion or pregnancy. Anti C is a rare cause of hemolytic disease of newborn and is very scarcely reported in the literature. The aim of the present case report of Hemolytic disease caused by Anti C antibody is to bring out the fact that antibodies other than anti D should be considered in cases that give a suggestive history but no evidence...

  16. Solidarity, authenticity and anti racism

    OpenAIRE

    Dinesh Wadiwel

    2009-01-01

    Anti Racist struggles often aim to construct alliances between groups under the banner of "solidarity" in order to achieve change. Using Foucault's Society Must be Defended lectures as a basis, this paper interrogates the difficulties associated with creating solidarity in anti racist movements, when conflict and war might be treated as the basis for racial stratifications. Following from this, it is argued that friendships in spite of differences are possible under the guise of "truce": that...

  17. Anti-hepatitis B virus effect of matrine-type alkaloid and involvement of p38 mitogen-activated protein kinase and tumor necrosis factor receptor-associated factor 6.

    Science.gov (United States)

    Chen, Jia-Xin; Shen, Hong-Hui; Niu, Ming; Guo, Yu-Ming; Liu, Xiao-Qiong; Han, Yan-Zhong; Zhang, Ya-Ming; Zhao, Yan-Ling; Bai, Bing-Ke; Zhou, Wen-Jun; Xiao, Xiao-He

    2016-04-01

    The matrine-type alkaloid, oxymatrine inhibits hepatitis B virus (HBV) replication but very little is known about these effects in other matrine-type alkaloids, including sophoridine and sophocarpine. Therefore, we compared the in vitro anti-HBV effects of matrine, oxymatrine, sophocarpine, and sophoridine by treating an HBV-transfected cell line (HepG2.2.15) with 0.4-1.6mM of the compounds for 24 or 72h. The levels of the HBV surface antigen (HBsAg) and e antigen (HBeAg) in the culture medium, as well as the intracellular and extracellular HBV DNA levels, were determined. Metabolomic analysis and detection of the mRNA level of p38 mitogen-activated protein kinase (MAPK), tumor necrosis factor receptor-associated factor (TRAF) 6, extracellular signal-regulated kinase (ERK) 1, NOD-like receptor family pyrin domain containing 10 (NLRP10), and caspase-1 were conducted in sophoridine-treated HepG2.2.15 cells. HepG2.2.15 cell exposure to 0.4-1.6mM sophocarpine or sophoridine for 24h reduced the HBsAg level of the medium more effectively than exposure to matrine and oxymatrine did, and reduced the HBeAg levels more effectively than these compounds did at 1.6mM. Sophoridine (0.4-1.6mM) reduced the cell medium HBV DNA levels more than the same concentrations of matrine, oxymatrine, or sophocarpine did. After 72h, 0.4 and 0.8mM sophoridine reduced HBsAg and intracellular HBV DNA levels more potently than matrine, oxymatrine, or sophocarpine did. Furthermore, sophoridine (0.8mM) potently reduced the cell medium HBeAg levels while the metabolomic analyses revealed that HepG2.2.15 cells exposed to 0.8mM sophoridine for 72h exhibited reduced cycloleucine and phytosphingosine levels. In addition, the mRNA expression analyses revealed that HepG2.2.15 cells exposed to 0.8mM sophoridine showed reduced levels of p38 MAPK, TRAF6, ERK1, NLRP10, and caspase-1. Sophoridine produced more potent anti-HBV effects than matrine, oxymatrine, and sophocarpine did. These effects may be related

  18. Anti-inflammatory and anti-cancer activity of mulberry (Morus alba L.) root bark

    OpenAIRE

    Eo, Hyun Ji; Park, Jae Ho; Park, Gwang Hun; Lee, Man Hyo; Lee, Jeong Rak; Koo, Jin Suk; Jeong, Jin Boo

    2014-01-01

    Background Root bark of mulberry (Morus alba L.) has been used in herbal medicine as anti-phlogistic, liver protective, kidney protective, hypotensive, diuretic, anti-cough and analgesic agent. However, the anti-cancer activity and the potential anti-cancer mechanisms of mulberry root bark have not been elucidated. We performed in vitro study to investigate whether mulberry root bark extract (MRBE) shows anti-inflammatory and anti-cancer activity. Methods In anti-inflammatory activity, NO was...

  19. Indicación de la determinación de los anticuerpos para los virus de la hepatitis C y de la hepatitis A en los protocolos de atención a los niños inmigrantes Indication of determination of antibodies against hepatitis C and A viruses in the protocol for the care of young immigrants

    Directory of Open Access Journals (Sweden)

    Rosa M. Masvidal Aliberch

    2010-08-01

    Full Text Available Objetivo: Valorar la determinación sistemática de los anticuerpos para el virus de la hepatitis C (Ac. VHC y para el virus de la hepatitis A (Ac. VHA dentro de los protocolo de atención a niños inmigrantes (PANI. Métodos: Estudio descriptivo, transversal y multicéntrico. Se determinaron los Ac. VHC y Ac.VHA, en niños de 6 meses a 15 años procedentes de países de baja renta que habían inmigrado hacía menos de 12 meses. Se registró la edad, el sexo y el país de origen. Se ha comparado el coste de la vacunación (con vacuna HA y vacuna HA-HB, precio de sanidad pública y precio de venta al público a todos los niños inmigrantes sin cribado, con el coste de la vacunación sólo a los no inmunes. Resultados: Se determinaron los Ac. VHC a 1055 niños/as, un caso resultó positivo: 0,09% (IC95%: 0-0,53%. Se determinaron los Ac. VHA a 992 niños/as, y fueron positivos el 38% (IC 95%: 35,0-41,1; en los de América Central y del Sur (n=352 el 34,9%; del Magreb (n=246 el 44,7%; de Indo-Pakistán (n=162 el 58,6%. Según el país de procedencia, la edad y el precio de la vacuna utilizada, es eficiente la determinación de los Ac. VHA previamente a la vacunación para el VHA. Conclusiones: La baja prevalencia de Ac. VHC en población infantil inmigrada no justifica su determinación sistemática. Valorar la determinación de los Ac. VHA en cada niño inmigrante en particular puede aumentar la eficiencia de los PANI.Objectives: To evaluate systematic determination of antibodies against hepatitis C virus (HCV and hepatitis A virus (HAV within the protocols for the care of young immigrants (PCYI. Methods: We performed a descriptive, cross-sectional, multicenter study. Antibodies against HCV and HAV were determined in children aged from 6 months to 15 years from low-income countries who had immigrated to Spain less than 12 months previously. Age, sex and country of origin were registered. The cost of vaccinating all immigrant children (with the HA

  20. Detección de anticuerpos para BVDV y BoHV-1 en llamas de la región de Tandil - Provincia de Buenos Aires Detection of antibodies to BVDV and BoHV-1 in llamas from Tandil - Province of Buenos Aires

    Directory of Open Access Journals (Sweden)

    P.E. Morán

    2010-12-01

    Full Text Available La llama (Lama glama es la especie predominante de camélidos sudamericanos de la República Argentina, con una poblaci