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Sample records for anticuerpos anti hbs

  1. KOEKSISTENSI HBsAg DAN ANTI-HBs DI MAKASSAR

    OpenAIRE

    uleng, -

    2015-01-01

    - Infeksi virus hipatitis B (VHB) merupakan penyakit endemik dengan insidensi tinggi di indonesia dan merupakan persoalan kesehatan global. Virus hepatitis B adalah suatu virus DNA yang berlapis ganda (double shelled) yang terdiri dari bagian luar yang dikenal sebagai hepatitis B surface antigen (HbsAG) dan bagian dalam atau hepatitis core antigen (HbcAg). Keberadaan HbsAg dalam serum merupakan tanda infeksi VHB. pembersihan VHB ditandai dengan didapatkannya anti-HBs dalam serum. Anti...

  2. Clinical and Virological Characteristics of Chronic Hepatitis B Patients with Coexistence of HBsAg and Anti-HBs.

    Science.gov (United States)

    Liu, Yong; Zhang, Le; Zhou, Jin-Yong; Pan, Jinshun; Hu, Wei; Zhou, Yi-Hua

    2016-01-01

    Coexistence of hepatitis B surface antigen (HBsAg) and antibody against HBsAg (anti-HBs) comprises an atypical serological profile in patients with chronic hepatitis B virus (HBV) infection. In this study, in total 94 patients with coexisting HBsAg and anti-HBs and 94 age- and sex-matched patients with positive HBsAg were characterized by quantitatively measuring HBsAg and HBV DNA, sequencing large S genes, and observing clinical features. Compared with common hepatitis B patients, the patients with coexisting HBsAg and anti-HBs had lower HBsAg and HBV DNA levels. These two groups had similar rate of pre-S deletion mutations. However, in patients with coexisting HBsAg and anti-HBs, more amino acid substitutions in the a determinant of S gene were observed in HBV genotype C, but not in genotype B. Fourteen patients with coexisting HBsAg and anti-HBs were followed up for an average of 15.5 months. There were no significant changes in the levels of HBsAg, anti-HBs, HBV DNA and ALT over the follow-up period. Compared with the baseline sequences, amino acid substitutions in the MHR of HBsAg occurred in 14.3% (2/14) patients. In conclusion, coexistence of HBsAg and anti-HBs may be associated with higher frequency of mutations in the a determinant of HBV genotype C.

  3. Clinical and Virological Characteristics of Chronic Hepatitis B Patients with Coexistence of HBsAg and Anti-HBs

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    Yong Liu; Le Zhang; Jin-Yong Zhou; Jinshun Pan; Wei Hu; Yi-Hua Zhou

    2016-01-01

    Coexistence of hepatitis B surface antigen (HBsAg) and antibody against HBsAg (anti-HBs) comprises an atypical serological profile in patients with chronic hepatitis B virus (HBV) infection. In this study, in total 94 patients with coexisting HBsAg and anti-HBs and 94 age- and sex-matched patients with positive HBsAg were characterized by quantitatively measuring HBsAg and HBV DNA, sequencing large S genes, and observing clinical features. Compared with common hepatitis B patients, the patien...

  4. Time to seroconversion of HBsAg to anti-HBs in individuals who lost HBsAg during follow-up.

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    Roushan, M R H; Mohammadpour, M; Baiany, M; Soleimani, S; Bijani, A

    2016-09-01

    To determine the time to appearance of antibody against hepatitis B surface antigen (anti-HBs) after clearance of hepatitis B surface antigen (HBsAg) in chronically infected individuals, we followed up 3963 cases with positive antibody against hepatitis B e antigen (anti-HBe) from 1991 to 2014. Of these, 101 (67 males, 34 females) lost HBsAg. These serocleared cases were checked every 6-month interval regarding HBsAg, anti-HBs, liver function tests, and liver sonography. Hepatitis B virus DNA was assessed at the time of seroclearance or the appearance of anti-HBs. The mean age of these patients at entry to this study was 34·4 ± 13 years. The mean follow-up duration until seroclearance of HBsAg was 6·6 ± 4·3 years. After the mean follow-up of 43·7 ± 45 months, anti-HBs appeared in 64 (63·4%) cases. The cumulative probabilities of anti-HBs appearance for 2, 5 and 10 years were 24·3%, 58% and 78·2%, respectively. The appearance of anti-HBs was associated with age ⩾35 years and seroclearance of HBsAg (hazard ratio 1·96, 95% confidence interval 1·32-3·38, P = 0·016) but not with sex. The results show that anti-HBs may develop in 78·2% of cases within 10 years of HBsAg clearance. Age ⩾35 years at HBsAg loss was associated with earlier development of anti-HBs.

  5. Efecto de un anticuerpo monoclonal anti CD20 (Rituximab) en trombocitopenia inmune.

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    Untama, José; Médico, Departamento de Hematología, Hospital Nacional Edgardo Rebagliati Martins – EsSalud. Lima.; Del Carpio, Daniel; Médico, Departamento de Hematología, Hospital Nacional Edgardo Rebagliati Martins – EsSalud. Lima.

    2012-01-01

    Objetivo: Describir la respuesta terapéutica con un anticuerpo monoclonal anti CD20 (Rituximab), en pacientes con Trombocitopenia Inmune (PTI). Material y métodos: Estudio retrospectivo, descriptivo y observacional tipo serie de casos. Se revisaron las historias clínicas de pacientes adultos con PTI que recibieron el anticuerpo monoclonal anti CD20 (Rituximab), desde diciembre 2005 hasta diciembre 2010. Se definió respuesta: conteo plaquetario >30 mil, por lo menos duplicar el conteo plaqu...

  6. Mutation in the S gene of hepatitis B virus and anti-HBs subtype-nonspecificity contributed to the co-existence of HBsAg and anti-HBs in patients with chronic hepatitis B virus infection.

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    Fu, Xiaochun; Chen, Jing; Chen, Huijuan; Lin, Jinpiao; Xun, Zhen; Li, Shiqi; Liu, Can; Zeng, Yongbin; Chen, Tianbin; Yang, Bin; Ou, Qishui

    2017-02-15

    The mechanism for the co-existence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) in chronic HBV infected patients remains controversial. This study aimed to explore the role of HBV S gene mutation and anti-HBs subtype-nonspecificity in patients with simultaneous HBsAg/anti-HBs positivity. Chronic HBV infections with (n = 145, group I) and without (n = 141, group II) anti-HBs were included. The S gene was amplified and sequenced. The neutralization experiment was used in group I patients' sera to determine the specificity of anti-HBs. Additionally, the HBV vaccinated persons' sera were used to estimate the neutralize capacity of anti-HBs against HBsAg in group I patients. Results showed that 2.63% (145/5513) chronic HBV infected patients had positive results for anti-HBs. HBsAg amino acid (aa) substitution rate in 35 patients of group I was significantly higher than that in 58 patients of group II (1.89% vs 0.95%, P anti-HBs in (74.29%, 26/35) patients was not directed to the subtypes of the co-existing HBsAg. Besides, some HBsAg variations in group I patients, sG145R mutation, inserted mutations, and continuous aa mutations within the major hydrophilic region (MHR), decreased the neutralized capacity of anti-HBs from HBV vaccinated persons. In conclusion, both of HBsAg mutation and anti-HBs subtype-nonspecificity contributed to the co-existence of HBsAg and anti-HBs in chronic HBV infection. HBV vaccine recipients may still have a risk of HBV infection when exposure to patients with simultaneous HBsAg/anti-HBs positivity.

  7. Differential reactivity of mouse monoclonal anti-HBs antibodies with recombinant mutant HBs antigens

    Institute of Scientific and Technical Information of China (English)

    Azam Roohi; Yaghoub Yazdani; Jalal Khoshnoodi; Seyed Mohammad Jazayeri; William F Carman; Mahmood Chamankhah; Manley Rashedan; Fazel Shokri

    2006-01-01

    AIM: To investigate the reactivity of a panel of 8 mouse anti-hepatitis B surface antigen (HBsAg) monoclonal antibodies (mAbs) using a collection of 9 recombinant HBsAg mutants with a variety of amino acid substitutions mostly located within the "a" region.METHODS: The entire HBs genes previously cloned into a mammalian expression vector were transiently transfected into COS7 cells. Two standard unmutated sequences of the ayw and adw subtypes served as controls. Secreted mutant proteins were collected and measured by three commercial diagnostic immunoassays to assess transfection efficiency. Reactivity of anti-HBs mAbs with mutated HBsAgs was determined by sandwich enzyme-linked immunosorbent assay (ELISA).RESULTS: Reactivity of anti-HBs mAbs with mutated HBsAgs revealed different patterns. While three mutants reacted strongly with all mAbs, two mutants reacted weakly with only two mAbs and the remaining proteins displayed variable degrees of reactivity towards different mAbs. Accordingly, four groups of mAbs with different but overlapping reactivity patterns could be envisaged. One group consisting of two mAbs (37C5-S7 and 35C6-S11) was found to recognize stable linear epitopes conserved in all mutants. Mutations outside the "a"determinant at positions 120 (P→S), 123(T→N) and 161(M→T) were found to affect reactivity of these mAbs.CONCLUSION: Our findings could have important implications for biophysical studies, vaccination strategies and immunotherapy of hepatitis B virus (HBV) mutants.

  8. Sequence analysis of the HBV S protein in Chinese patients with coexisting HBsAg and anti-HBs antibodies.

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    Ding, Feng; Yu, Hong-Gang; Li, Yan-Xia; Cui, Ning; Dai, Jin-Fen; Yu, Jie-Ping

    2015-12-01

    The coexistence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) in patients with hepatitis B virus (HBV) infection has been discovered and explained for several decades, but debate still exists. This study was to explore the relationship between this special serological pattern and mutations in S gene region. Fifteen patients with coexisting HBsAg and anti-HBs were selected as the experimental group, and 27 patients with HBsAg positive only were selected as the control group. The S gene region was amplified and sequenced. No significant differences were observed between the two groups with regard to age, gender, alanine aminotransferase level, HBsAg titer, genotype, and HBV DNA level. The patients from the two groups were infected with HBV of the genotype B and C. Compared with the control group, the experimental group showed a higher variability in amino acid within the N-terminal region and the MHR, especially the "a" determinant. The most frequent change in patients from the experimental group was located at positions s126. The coexistence of HBsAg and anti-HBs might be associated with the increased amino acid mutations in the "a" determinant. Further studies should be performed to determine the clinical implication of this serological pattern, including the binding of anti-HBs to HBsAg, escape from immune system, and efficacy of antiviral therapy.

  9. Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs

    Institute of Scientific and Technical Information of China (English)

    Hai-Ying Lu; Zheng Zeng; Xiao-Yuan Xu; Nai-Lin Zhang; Min Yu; Wei-Bo Gong

    2006-01-01

    AIM: To investigate the clinical significance and presence of mutations in the surface (S) and overlapping polymerase gene of hepatitis B patients with coexisting HBsAg and anti-HBs.METHODS: Twenty-three patients with chronic hepatitis B were studied. Of the 23 patients, 11 were both positive for hepatitis B virus (HBV) surface antigen (HBsAg) and antibody to HBV surface antigen (anti-HBs), 12 were negative for anti-HBs while positive for HBsAg. DNA was extracted from 200 μL serum of the patients. Nucleotide of the surface and overlapping polymerase gene from HBV-infected patients was amplified by PCR, and the PCR products were sequenced.RESULTS: Forty-one mutations were found within the surface gene protein of HBV in 15 patients (10 with coexisting HBsAg and anti-HBs). Six (14.6%) out of 41mutations were located at "α" determinant region in 5 patients (4 positive for HBsAg and anti-HBs). Eleven mutations (26.8%) occurred in the downstream or upstream of "α" determinant region. Lamivudine (LMV)-selected mutations were found in three patients who developed anti-HBs, which occurred in amino acid positions (196, 198, 199) of the surface protein and in YMDD motif (M204T/V) of the polymerase protein simultaneously. Presence of these mutations did not relate to changes in ALT and HBV DNA levels.CONCLUSION: Besides mutations in the "α" determinant region, mutations at downstream or upstream of the "α" determinant region may contribute to the development of anti-HBs. These mutations do not block the replicating competency of HBV in the presence of high titer of anti-HBs.

  10. Mutation in the S gene a determinant of the hepatitis B virus associated with concomitant HBsAg and anti-HBs in a population in Northeastern Brazil.

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    de Campos Albuquerque, Ingrid; Sousa, Marinilde Teles; Santos, Max Diego Cruz; Nunes, Jomar Diogo Costa; Moraes, Maria Josélia Diniz; Gomes-Gouvêa, Michele Soares; Pinho, João Renato Rebelo; Carrilho, Flair José; Fonseca, Lena Maria Barros; de Sousa Paiva Ferreira, Adalgisa

    2017-03-01

    Mutations in the a determinant of S gene may develop co-existence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) in the serum of infected hepatitis B virus (HBV) individuals. Mutations in this region may change the antigenicity of HBsAg, which in turn, lead to escape of neutralizing action of anti-HBs antibodies. This study identified individuals with concomitant HBsAg and anti-HBs serological markers in individuals of Maranhão, Northeastern Brazil. Samples from a population-based study were evaluated for HBsAg, anti-HBs, and anti-HBc, and those that tested positive for simultaneous HBsAg and anti-HBs were submitted to HBV DNA quantification and S gene characterization by Sanger sequencing. Mutations were investigated in the a determinant located in major hydrophilic region (MHR) of the S gene. Among 3,984 samples analyzed, 92 (2.3%) were positive for HBsAg and three had the atypical HBsAg and anti-HBs-positive profile (3.26%). The frequency of HBsAg and anti-HBs co-existence was similar to previous studies. Only one individual harbored mutation in the S gene a determinant associated with this profile. Little is known about this phenomenon; however, studies as ours may contribute for future enlightenment of this important issue. J. Med. Virol. 89:458-462, 2017. © 2016 Wiley Periodicals, Inc.

  11. Epidemiological characteristics of the carriers with coexistence of HBsAg and anti-HBs based on a community cohort study.

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    Pu, Z; Li, D; Wang, A; Su, H; Shao, Z; Zhang, J; Ji, Z; Gao, J; Choi, B C K; Yan, Y

    2016-04-01

    The coexistence of HBsAg and anti-HBs is an atypical serological pattern in HBV infection. There is no epidemiological characteristics of this serological pattern in the community and there is controversy over the molecular mechanisms underlying this pattern. We investigated the epidemiological characteristics of the carriers with HBsAg and anti-HBs in a longitudinal community cohort study. The prevalence of this atypical serological pattern was 2.93% (122/4169) in HBsAg-positive populations. The prevalence progressively increased with age from 40 to 70 years old. The rate of HBeAg positive and detectable HBV DNA were both significantly higher in carriers with this pattern than in carriers who were HBsAg positive but anti-HBs negative (26/122 verse 598/4047, P = 0.046; 86/122 verse 275/529,P anti-HBs, 14.81% of the carriers lost their anti-HBs. Viral sequencing showed that carriers with coexistence of HBsAg and anti-HBs had higher numbers of residue changes within the S gene than carriers who were HBsAg positive but anti-HBs negative (2.42 verse 1.33 changes per 100 residues, P anti-HBs is a unique serological pattern which may be associated with an increased risk of adverse clinical outcome and may be related to HBsAg immune variants which have genotypic heterogeneity.

  12. Can HB vaccine yield a booster effect on individuals with positive serum anti-HBs and anti-HBc markers?

    Institute of Scientific and Technical Information of China (English)

    Ru-Xiang Wang; Christina Y. Shi; Ying Guo; Chang-Hong Yang; Yu Song; Juan Chen; Fu-Sheng Pang; Shao-Ping Lei; Xiao-Ming Jia; Jin-Ying Wen

    2004-01-01

    AIM: To evaluate if HB vaccination can yield a booster effecton the anti-HBs level of those naturally acquired HBV positivemarkers.METHODS: Sera were collected from 1399 newly enrolleduniversity students aged between 18-20 years at theentrance medical examination in 2001. Forty-four students(28 males and 16 females) with positive serum anti-HBs and anti-HBc markers served as an observation group and another 44 students (24 males and 20 females) without any HBV markers as the control. HB vaccination was given to all the students without positive serum HBs Ag according to 0,1, 6 month regimen and the peripheral venous blood was sampled from those of both observation and control groups for anti-HBs detection one month after the second and third doses. Anti-HBs levels were measured by ELISA.RESULTS: The seroconversion rate of anti-HBs in the control group was 100% after the second dose, but the geometric mean titers (GMTs) were low. The tendency of serum anti-HBs changes after the 3rd dose was completely different between the two groups. Although more than half of those with positive anti-HBs and anti-HBc showed a mild increase of anti-HBs levels after the 2nd boosting dose (mean anti-HBs level was 320:198 mIU), but the increase of serum anti-HBs titer was much smaller than that in the control group. The averages of their initial serum anti-HBs levelsand the levels after the 2nd and 3rd doses were 198, 320 and 275 mIU respectively. All the subjects from the control group had an obvious increase in their serum anti-HBs levels which was nearly 4 times the baseline level (302:78 mIU).CONCLUSION: HB vaccination can not enhance anti-HBs levels in those with positive serum anti-HBs and anti-HBc markers.

  13. Nationwide seroepidemiology of hepatitis B virus infection in South Korea in 2009 emphasizes the coexistence of HBsAg and anti-HBs.

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    Lee, Byung Seok; Cho, Yong Kyun; Jeong, Sook-Hyang; Lee, Joon Hyeok; Lee, Don; Park, Neung Hwa; Ki, Moran

    2013-08-01

    Hepatitis B virus (HBV) infection is the major cause of chronic liver disease in Korea. This study investigated the seroprevalence of HBV infection with an emphasis on the coexistence of hepatitis B surface antigen (HBsAg) and antibody (anti-HBs). In all, 290,212 people undergoing health check-up examinations in 29 institutions during 2009 were recruited. The crude seroprevalences of HBsAg and anti-HBs was adjusted by age, sex, and geographic area using the 2009 estimated population of Korea. The adjusted seroprevalences of HBsAg and anti-HBs was 4.0% and 73.5%, respectively. Males showed higher HBsAg positivity and lower anti-HBs positivity than females (Panti-HBs coexisted in 0.1% of the total subjects and in 2.9% of the HBsAg-positive group, showing distinct age distribution and higher alanine aminotransferase levels than those of the group positive for only HBsAg. In conclusion, the seroprevalence of HBsAg and anti-HBs in Korea varies significantly by age, sex and geographical location and coexisted in 2.9% of HBsAg-positive subjects. Continuous monitoring of seroepidemiology may facilitate the eventual eradication of HBV infection.

  14. Hepatitis B virus surface antigen (HBsAg and antibody (anti-HBs forming immune complexes in fulminant hepatitis

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    Soares Manoel C.P.

    1999-01-01

    Full Text Available This paper reports an unusual pattern of serological HBV markers and the presence of HBsAg/anti-HBs immune complexes in serum samples from two patients with fulminant hepatitis from the Brazilian Western Amazon Basin. The diagnosis was made by both serologic tests and demonstration of antigen/antibody complexes by transmission electron microscopy. Concurrent Delta virus superinfection is also discussed.

  15. Detection of HBsAg-anti-HBs complex by double-labelling immunofluorescence method%免疫荧光双标记技术检测胎盘HBsAg-抗-HBs复合物

    Institute of Scientific and Technical Information of China (English)

    王歆; 徐德忠; 刘蓬勃; 李玉松; 王素萍

    2001-01-01

    AIM To find out which form Hepatitis B Virus (HBV) takes in the infection of the first layer cell of placenta barrier-trophoblast cells. METHODS HBsAg-anti-HBs complexes in HBsAg seropositive maternal placenta tissues were tested by double-labeling immunofluorenscence method visualized with laser scanning confocal microscope. RESULTS HBsAg-anti-HBs complexes were found mainly on trophoblast cells (TC) of HBsAg seropositive maternal placenta tissues. CONCLUSION HBsAg and anti-HBs are formed as HBsAg-anti-HBs complexes before entry into trophoblast cells, which may result in the infection of the first layer cell of placenta barrier.%目的探讨乙肝病毒(HBV)以何种形式感染胎盘滋养层细胞. 方法通过免疫荧光双标记技术检测12例血清 HBsAg阳性母亲的胎盘滋养层细胞HBsAg-抗-HBs复合物. 结果血清学HBsAg阳性母亲胎盘滋养层细胞中存在HBsAg-抗-HBs复合物. 结论提示HBV可能通过HBsAg与抗-HBs结合成复合物的方式进入胞内,导致胎盘屏障的第一层细胞受到HBV感染.

  16. Evolution of full-length genomes of HBV quasispecies in sera of patients with a coexistence of HBsAg and anti-HBs antibodies.

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    Zhou, Tai-Cheng; Li, Xiao; Li, Long; Li, Xiao-Fei; Zhang, Liang; Wei, Jia

    2017-04-06

    Although the evolutionary changes of viral quasispecies are correlated to the pathological status of a disease, little is known in the coexistence of hepatitis B surface antigen (HBsAg) and antibodies to these antigens (anti-HBs). To examine evolutionary changes in hepatitis B virus (HBV) and their relationship to the coexistence of HBsAg and anti-HBs antibodies, HBV genomes in patients with a coexistence of HBsAg and anti-HBs antibodies (experimental group) and HBsAg positive without anti-HBs (control group) were assessed. Our results showed that quasispecies diversity was significantly higher in the experimental group for large HBsAg (LHBsAg), middle HBsAg (MHBsAg), and HBsAg genes. LHBsAg harbored dN/dS values eight times higher in the experimental group; however, the mean dN/dS ratios in genes HbxAg, Pol and PreC/C of the experimental patients had an opposite trend. Phylogenetic trees in the experimental group were more complex than the control group. More positive selection sites, mutations and deletions were observed in the experimental group in specific regions. Furthermore, several amino acid variants in epitopes were potentially associated with the immune evasion. In conclusion, cumulative evolutionary changes in HBV genome that facilitate immune evasion provide insights into the genetic mechanism of a coexistence of HBsAg and anti-HBs antibodies.

  17. Frecuencia de anticuerpos anti Toxoplasma gondii en gestantes de Cúcuta, Colombia

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    Cárdenas, Denny; Lozano, Claudia; Castillo, Zaida; Cedeño, Jessenia; Galvis, Viannis; Rios, Jessica; Tórres, Madalhene

    2016-01-01

    La toxoplasmosis es una infección ocasionada por Toxoplasma gondii, peligrosa durante la gestación. La presencia de anticuerpos IgG específicos implica contacto previo individuo-parásito, mientras que la detección de IgM anti-T. gondii, es considerada marcador de infección aguda, fase en la que se incrementa además el título IgG. Objetivo: Determinar la frecuencia y título de anticuerpos contra Toxoplasma gondii en gestantes. Material y métodos: Estudio transversal, se determinó el título de ...

  18. The effects of booster vaccination on hepatitis B vaccine in anti-HBs negative infants of HBsAg-positive mothers after primary vaccination.

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    Gu, Hua; Yao, Jun; Zhu, Wei; Lv, Huakun; Cheng, Suyun; Ling, Luoya; Xia, Shichang; Chen, Yongdi

    2013-06-01

    The purpose of this study was to investigate the changes in anti-HBs IgG levels after booster vaccinations in anti-HBs negative infants of HBsAg-positive mothers. After primary vaccination, the immunization effects of different dosages of booster vaccinations of hepatitis B vaccine (CHO) were compared. A group of 472 newborns were vaccinated with three-dose hepatitis B vaccine at birth, 1 mo and 6 mo of age. Blood serum was collected within 6-12 mo after the third dose, and HBsAg, anti-HBs and anti-HBc levels were determined. Of this group, 101 infants who were both anti-HBs and HBsAg negative were revaccinated with 20 μg hepatitis B vaccine (CHO), and their antibody titers were monitored. Among these 101 infants, the anti- HBs positive rates (defined as anti-HBs ≥ 100 mIU/ml) differed after the first and the third dose (79% and 90%, respectively (panti-HBs GMTs after booster vaccination were 10-fold larger than those before booster vaccination. We conclude that a single booster dose is generally adequate for infants of HBsAg-positive mothers, whereas a further booster dose should be given for non-responders.

  19. The seroprevalences of HBs Ag and anti-HCV in pregnant women in Ankara.

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    Altinbas, Sibel; Erdogan, Mine; Danişman, Nuri

    2010-02-01

    In the previous decade, the prevalence of HBs-Ag positivity and the anti-HCV positivity declined in Turkey. We aimed to investigate the prevalences of HBs Ag and anti-HCV positivity in pregnant women in Ankara, the capital city of Turkey, while the vertical transmission should be important way of HBV and HCV transmission. HBs-Ag positivity was determined 2.8% (102) out of 4,700 pregnant women, and 0.1% (6) out of them were positive for anti-HCV. The prevalences of HBs Ag and anti-HCV were both similar to the rate of that seen in western region of Turkey.

  20. Inmunogenicidad de una vacuna recombinante anti-HBs en trabajadores de la salud, del Instituto de Medicina Legal de Colombia

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    Heber Siachoque

    2007-12-01

    Full Text Available Objetivo: Evaluar la inmunogenicidad de la vacuna recombinante Hepavax-Gene para hepatitis B desde la última dosis administrada en trabajadores del INML.Materiales y métodos: Estudio de corte transversal en 603 trabajadores de la salud con mínimo 3 dosis de vacuna recombinante (0, 1, 6 meses donde se midieron los niveles de anticuerpos anti-HBs con la técnica de ELISA entre diciembre de 2000 y enero de 2001 desde la aplicación de la última dosis de la vacuna, que varió entre 1 y 6 años.Resultados: El grupo de estudio lo conformaron 344 hombres y 259 mujeres, con un promedio de edad de 38.8±7.3 años. El nivel de protección fue 90.7% (>10 U/l que disminuyó significativamente con el tiempo de aplicación de la última dosis de la vacuna (p45 (RRI=3.58, IC 95%:1.83, 6.99 con respecto a <40 años. Conclusión: La vacuna recombinante Hepavax-Gene anti-HBs tiene alta efectividad en los trabajadores de la salud (90.7% aunque presenta disminución de protección a mayor tiempo de aplicación de la última dosis y al aumentar la edad del trabajador.

  1. Predictive factors for anti-HBs status after 1 booster dose of hepatitis B vaccine.

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    Lu, I-Cheng; Jean, Mei-Chu Yen; Lin, Chi-Wei; Chen, Wei-Hung; Perng, Daw-Shyong; Lin, Chih-Wen; Chuang, Hung-Yi

    2016-09-01

    In Taiwan, infants need to receive 3 doses of hepatitis B virus (HBV) vaccine under the public health policy from the government. However, there are many young adults who even though received complete HBV vaccination in their childhood would lose the positive response of anti-hepatitis B surface antibody (HBs) and need the booster dose of HBV vaccine. The aim of our study is to determine the powerful predictive factor for screening the candidates who need only 1 booster dose of HB vaccine then they can regain positive postbooster anti-HBs status (≧10 mIU/mL) or protective postbooster anti-HBs status (≧100 mIU/mL).We recruited 103 university freshmen who were born after July 1986 with complete HBV vaccination in childhood, but displayed negative results for hepatitis B surface antigen and anti-HBs levels at their health examinations upon university entry. They received 1 booster dose of HB vaccine, and their anti-HBs titers were rechecked 4 weeks after the booster administration. Multivariate analysis logistic regression for positive postbooster anti-HBs status (≧10 mIU/mL, model 1) and protective postbooster anti-HBs status (≧100 mIU/mL, model 2) was done with predictive factors of prebooster anti-HBs level, body mass index, serum glutamate pyruvate transaminase level, and sex.Twenty-four students got positive postbooster anti-HBs status (10-100 mIU/mL) and 50 students got protective postbooster anti-HBs status (≧100 mIU/mL). In the model of multivariate analysis logistic regression for positive postbooster anti-HBs status (≧10 mIU/mL), prebooster anti-HBs level was the strongest predictive factor. The odds ratio was 218.645 and the P value was 0.001. Even in the model of multivariate analysis logistic regression for protective postbooster anti-HBs status (≧100 mIU/mL), prebooster anti-HBs level was still the strongest predictive factor, but the odds ratio of a protective booster effect was 2.143, with 95% confidence interval between 1

  2. [Atypical serological profiles in hepatitis B infections: investigation of S gene mutations in cases with concurrently positive for HBsAg and anti-HBs].

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    Aydın, Neriman; Kırdar, Sevin; Uzun, Nilgül; Eyigör, Mete; Sayan, Murat

    2016-10-01

    Hepatitis B virus (HBV) causes different clinical manifestations, ranging from asymptomatic carriage to fulminant or chronic hepatitis. Serological tests are widely used for the diagnosis of HBV infections to detect viral markers. However, facing with atypical serological profiles in some patients leads to problems in interpreting of the results and management of the patients. The aims of this study were to investigate the atypical serologic profiles seen in patients screened for HBV infection and the S gene mutations in patients with concurrent positivity of HBsAg and anti-HBs. A total of 592 sera from patients (332 male, 260 female; age range: 13-84 years, mean age: 43.9 years) prediagnosed as HBV infection between January to September 2013, and screened for HBV markers (HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc-IgM, anti-HBc-total and HBV-DNA) were included in the study. Of those samples 364 were screened only for HBsAg and anti-HBs markers. S gene mutations were investigated by direct sequencing method in sera which were concurrent positive for HBsAg and anti-HBs. In our study, 5.2% (31/592) of the sera yielded atypical serologic profiles. Of these 13 cases were concurrently positive for HBsAg and anti-HBs; nine were HBeAg positive, anti-HBe and HBV-DNA negative; eight were HBeAg, anti-HBe and HBV-DNA positive; and one was HBsAg and anti-HBs negative, anti-HBe and HBV-DNA positive. The rate of concurrent positivity of HBsAg and anti-HBs was 3.6% (13/364), while 76.9% (10/13) of those cases were also positive for HBV-DNA. DNA sequencing was performed for seven out of 10 samples which were positive for HBsAg, anti-HBs and HBV-DNA, however three samples were not used because of the low amounts. Sequence analysis of seven samples showed S gene mutations in two samples, one was sS143L with sS193L, a HBV vaccine escape mutation, and the other was sP120R, a HBV immune escape mutation. Of the patients 2.7% (10/364) was negative for both HBsAg and anti-HBs; in which

  3. Effects of Risk Factors on Anti-HBs Development in Hepatitis B Vaccinated and Nonvaccinated Populations.

    Science.gov (United States)

    Shaha, Modhusudon; Hoque, Sheikh Ariful; Ahmed, Mohammad Firoz; Rahman, Sabita Rezwana

    2015-05-01

    Hepatitis B infection is still a major global health problem even though safe and effective vaccines have been available for more than 30 years. Although development of protective antibody to hepatitis B surface antigen (anti-HBs) is a common phenomenon after vaccination as well as natural infection, sometimes it does not appear even after complete vaccination. In the present study, whether the impairment of the development of anti-HBs in naturally infected and/or vaccinated populations is associated with immunomodulating risk factors (i.e., age, gender, smoking, and diabetes) and/or other risk factors (i.e., socioeconomic status, dental, and saloon exposure) was investigated through a cross-sectional study. Among 204 nonvaccinated patients, 132 (64.7%) tested positive for anti-HBc, indicating that they had been exposed to hepatitis B virus (HBV) at least once in their lifetime. Exposure to HBV (anti-HBc positive) was significantly higher among low-income people, dental exposed, and saloon users. Among anti-HBc positive patients, only 44 (33.3%) developed natural immunity with anti-HBs. Impairment in anti-HBs formation was found to be significantly high among cigarette smokers. However, no significant association of anti-HBs development was observed with age, gender, socioeconomic status, diabetes, dental exposure, and using saloon. Consistently, the frequency of developing protective anti-HBs (≥10 IU/L) among a vaccinated population was almost nine times less among smokers. These data suggest that anti-HBs development, either naturally or after vaccination, is significantly lower among smokers. It emphasizes the need to check the anti-HBs status in smokers after vaccination, and a booster vaccination should be administered if the anti-HBs antibody titer decreases below the protective level.

  4. Seroprevalencia de anticuerpos anti-Toxoplasma gondii en pacientes con afecciones Oncohematológicas

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    Dailín Cobos Valdes

    2009-01-01

    Full Text Available Se analiza la reactividad de 23 muestras de suero de pacientes con hemopatías malignas provenientes de la sala de hematología del Hospital Clínico Quirúrgico de Holguín, mediante la técnica de Inmunofluorescencia Indirecta, reportándose un 65,2% de seroprevalencia. La Toxoplasmosis es una enfermedad causada por el agente etiológico Toxoplasma gondii, parásito intracelular obligado ampliamente distribuido en todo el mundo y capaz de infectar un gran número de huéspedes, incluida la especie humana. Numerosas han sido las investigaciones sobre la presencia de anticuerpos anti- Toxoplasma gondii en individuos inmunodeficientes, donde los pacientes con enfermedades oncohematológicas son un grupo representativo dentro de esta población.

  5. Effects of hepatitis B vaccine boosters on anti-HBs-negative children after primary immunization.

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    Lu, Shunshun; Ren, Jingjing; Li, Qian; Jiang, Zhenggang; Chen, Yongdi; Xu, Kaijin; Ruan, Bing; Yang, Shigui; Xie, Tiansheng; Yang, Linna; Li, Jing; Yao, Jun

    2016-12-01

    This study was aimed at evaluating the changes of hepatitis B surface antibody (anti-HBs) titer after booster vaccinations in 5-15-year-old children with negative antibodies (anti-HBs level: Group I, anti-HBs ≥10.0 mIU/mL) among 225 subjects at the 4 time-points were 93.8%, 100%, 83.6% and 73.4%, respectively (χ(2) = 90.29, p anti-HBs geometric mean titer (GMT) in Group III were always higher than those in the other 2 groups (all p < 0.05). The immune effect of a 3 -dose booster revaccination is good, and the booster-induced immune response was correlated with the pre-booster titer level, and ≥1.0 mIU/mL ensuring a robust positive response, whereas titers below this value may indicate the need for a course of booster vaccination.

  6. Coexistence of hepatitis B surface antigen (HBs Ag) and anti-HBs antibodies in chronic hepatitis B virus carriers: influence of "a" determinant variants.

    Science.gov (United States)

    Lada, Olivier; Benhamou, Yves; Poynard, Thierry; Thibault, Vincent

    2006-03-01

    In chronic hepatitis B (CHB), the persistence of hepatitis B surface antigen (HBs Ag) is sometimes associated with antibodies (Ab) to HBs (anti-HBs). To assess the hypothesis of the selection of HBs Ag immune escape variants in CHB patients, the variability of the HBV S gene was determined for patients persistently carrying both HBs Ag and anti-HBs antibodies and patients solely positive for HBs Ag. We selected 14 patients who presented both markers (group I) in several consecutive samples and 12 patients positive for HBs Ag only (group II). The HBs Ag-encoding gene was amplified and cloned, and at least 15 clones per patient were sequenced and analyzed. The number of residue changes within the S protein was 2.7 times more frequent for group I than for group II patients and occurred mostly in the "a" determinant of the major hydrophilic region (MHR), with 9.52 versus 2.43 changes per 100 residues (P = 0.009), respectively. Ten patients (71%) from group I, but only three (25%) from group II, presented at least two residue changes in the MHR. The most frequent changes in group I patients were located at positions s145, s129, s126, s144, and s123, as described for immune escape variants. In CHB patients, the coexistence of HBs Ag and anti-HBs Ab is associated with an increase of "a" determinant variability, suggesting a selection of HBV immune escape mutants during chronic carriage. The consequences of this selection process with regard to vaccine efficacy, diagnosis, and clinical evolution remain partially unknown.

  7. Prevalencia de anticuerpos anti envoltura nuclear y sus isotipos en sueros positivos para anticuerpos antinucleares Prevalence of antinuclear envelope antibodies and their isotypes in sera positive for antinuclear antibodies

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    Miriam Arcavi

    2006-08-01

    Full Text Available Los anticuerpos antinucleares detectados por inmunofluorescencia indirecta en células HEp-2 presentan una gran variedad de imágenes, entre ellas el patrón de envoltura nuclear que suele ser un hallazgo poco frecuente. Se procesaron 2594 sueros en los cuales se detectó un 37.6% de anticuerpos antinucleares. La prevalencia de anticuerpos anti-envoltura nuclear (ANEA fue del 1.2% presentando una alta asociación con hepatopatías autoinmunes (83% y baja con lupus eritematoso sistémico. En los 21 sueros de los pacientes que presentaron ANEA no se detectaron anticuerpos anti-ADNn hallándose 28.6% de anticuerpos anti-músculo liso y 19% de anticuerpos anti-mitocondriales. El corte triple de tejido de rata mostró ser un sustrato menos sensible que HEp-2 para la detección de ANEA. Al utilizar conjugados dirigidos contra diferentes isotipos de anticuerpos para la detección de ANEA, se encontró: 90.5% de IgG, 66.6% de IgA y 9.5% de IgM. Dos de los pacientes presentaron ANEA-IgA a altos títulos (³1:160 en ausencia de ANEA-IgG. En este trabajo se destaca la importancia de realizar pruebas complementarias que detecten anticuerpos anti-músculo liso, anti-mitocondriales y anti-ADNn, para orientar el diagnóstico clínico de los pacientes que presentan ANEA. Además, sostiene la postura de utilizar como conjugado para IFI-HEp2 anticuerpos anti-inmunoglobulinas totales en lugar de anti-IgG hasta tanto se clarifique el rol que juegan los anticuerpos IgA en estas enfermedades autoimunes.Antinuclear antibodies detected in HEp-2 cells by indirect immunofluorescence assay display a great variety of images, including the nuclear envelope pattern. This is quite a less frequent finding. Two thousand five hundred and ninety-four sera were processed, and 37.6% of ANA were detected. The prevalence of anti-nuclear envelope antibodies (ANEA was of 1.2%, with a high association with autoimmune liver diseases (83% and a low association with systemic lupus

  8. Seropositivity of HBsAg, anti-HCV and anti-HIV in preoperative patients

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    Berrin Karaayak Uzun

    2014-12-01

    Full Text Available Objective: The infections caused by human immunodeficiency virus (HIV, hepatitis B (HBV and C (HCV viruses pose a serious occupational risk for the healthcare workers especially those in emergency services, laboratories and surgery wards. Vaccination and establishment of the strict biosafety procedures are the main principles to prevent blood-borne infections in healthcare workers. Additionally, serological screening of the preoperative patients could decrease the risk for exposure. In this study, we aimed to determine the seroprevalence of HBsAg, anti-HCV, anti-HIV 1/2 in preoperative patients. Methods: Hospital automation records were evaluated retrospectively for 4.367 patients who were scheduled for surgery and scanned for anti-HIV 1/2, HBsAg and anti-HCV as preoperative procedures in the preparation period of operation between January 2012 and December 2012. Results: HBsAg positivity rate was found in 7.7% (n=336, anti-HCV positivity rate was found in 2.3% (n=101. A two (0.05% of five patients were positive for anti-HIV 1/2 was found positive verification test and the other three samples were accepted as false positive test results. Conclusion: All healthcare workers must be trained about occupational diseases and vaccinated against Hepatitis B. Universal precautions must be strictly followed particularly in the operating room. In addition, all patients should be considered as potential carriers regarded as a carrier of the potential for infection. J Clin Exp Invest 2013; 4 (4: 449-452

  9. Characterization of C69R variant HBsAg: effect on binding to anti-HBs and the structure of virus-like particles.

    Science.gov (United States)

    Hadiji-Abbes, Nadia; Mihoubi, Wafa; Martin, Marta; Karakasyan-Dia, Carole; Frikha, Fakher; Gergely, Csilla; Jouenne, Thierry; Gargouri, Ali; Mokdad-Gargouri, Raja

    2015-10-01

    Several variants of the major "a" determinant of the HBsAg, the main target of HBV neutralization by antibodies, have been described. However, mutations outside this region have not been as thoroughly investigated. During the genotyping of HBV from Tunisian patients with chronic hepatitis B, we identified a variant with a C69R substitution in the cytosolic loop of the S protein, resulting in a change in the hydrophobicity profile compared to the wild-type HBsAg. Wild-type and mutant HBsAgs were produced in Saccharomyces cerevisiae and recombinant proteins were tested for their ability to correctly self-assemble into virus-like particles (VLPs), and their ability to bind to HBs antibodies. The C69R substitution resulted in a decrease in binding to commercial anti-HBs antibodies, and although the variant appeared to assemble properly into VLPs, the average size of the particles was larger than that of the wild-type HBsAg. Prediction of the tertiary structure of the C69R mutant revealed a change in the first (aa 60-70) and the second loop (aa 110 to 120) compared to the wild-type protein. Furthermore, we showed by an isothermal titration calorimetry assay that the interaction between the wild-type HBsAg and the anti-HBs antibody was exothermic, whereas that with the mutant C69R was endothermic, indicating an effect on the binding affinity.

  10. Risk association of hepatocellular carcinoma with S-gene additional N-glycosylation mutation of hepatitis B virus in HBsAg and anti-HBs coexistent patients

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    Yan QIAO

    2016-12-01

    Full Text Available Objective  To investigate the association of additional N-glycosylation mutation in major hydrophilic region (MHR of hepatitis B virus (HBV S gene with the risk of hepatocellular carcinoma (HCC in HBsAg and anti-HBs coexistent patients. Methods  A total of 284 patients with coexistence of HBsAg and anti-HBs were enrolled in this study, who were admitted in 302 Hospital of PLA from July 2009 to June 2016. HBV DNA was extracted from serum samples and subjected to nested PCR for full-length S-gene sequencing. The association of MHR additional N-glycosylation mutation and clinical parameters with HCC occurrence risk was analyzed. Specifically, the additional N-glycosylation mutation was dynamically analyzed pre-and post-HCC occurrence for 18 patients. Results  Multivariate analysis showed that age >40 years, HBsAg >median, HBeAg negativity, and additional N-glycosylation mutation in MHR were associated with HCC occurrence for the HBsAg and anti-HBs coexistent patients (OR=4.281, 95%CI 1.843-9.941, P=0.001; OR=3.146, 95%CI 1.633-6.060, P=0.001; OR=2.097, 95%CI 1.010-4.357, P=0.047; and OR=4.381, 95%CI 1.842-10.417, P=0.001. In contrast, ALT, anti-HBs, anti-HBe, and HBV DNA levels had no significant association with HCC occurrence. Dynamical analysis showed that the additional N-glycosylation mutation had already developed 1-4 years prior to HCC occurrence in the 8 of 18 observed patients. Conclusion  Additional N-glycosylation mutation in MHR of HBV S gene had close association with HCC occurrence in HBsAg and anti-HBs coexistent patients, suggesting that HBsAg and anti-HBs coexistence and additional N-glycosylation mutation together could serve as a predictive indicator for HCC occurrence in chronic HBV-infected patients. DOI: 10.11855/j.issn.0577-7402.2016.11.08

  11. Occult HBV infection in anti-HBs-positive young adults after neonatal HB vaccination.

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    Xu, Libin; Wei, Yong; Chen, Taoyang; Lu, Jianhua; Zhu, Chang-Lin; Ni, Zhengping; Huang, Fei; Du, Jun; Sun, Zongtang; Qu, Chunfeng

    2010-08-23

    Previous follow-up on our neonatal HB vaccination cohorts with 80,000 individuals in Qidong, China, showed significant protective efficacy of immunization against HBV infection in childhood. However, some vaccinees were found to be HBsAg negative, but anti-HBs positive and anti-HBc positive at age 10-11 years. To study this phenomenon, 2919 vaccinees at age 19-21 years were sampled from the cohort. HBsAg(-), anti-HBs(+) and anti-HBc(+) were found in 124/2919 (4.2%) of the vaccinees. HBV DNA was detectable in 81/106 sample sera by using nested PCR. The PreS-S regions of HBV were sequenced in 41 randomly sampled sera. All the HBV isolates were HBV genotype C. Twenty one isolates (21/41, 51.2%) were identical to an HBV isolated in this area (GU434374). Only 4/41 (9.8%) showed mutations at the "a" epitope and three of them were G145A. The other mutations were found outside of the "a" epitope. Most of the sera contained anti-HBs(+) and anti-HBc(+) status, who received neonatal vaccination in Qidong.

  12. HbsAg and antiHCV seroprevalence in an Eastern province of Turkey

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    Özlem Demirpençe

    2016-03-01

    Full Text Available Objective: The aim of this study was to investigate the seroprevalance of the Hepatitis B (HBV and Hepatitis C (HCV viruses among patients living in Tunceli province who had been admitted to the Tunceli State Hospital. Method: The seropositivity rates of hepatitis B surface antigens (HBsAg, n:3640, antibodies to hepatitis B surface antigen (anti-HBs, n:3941 and HCV antibodies (anti-HCV, n:3489 were retrospectively studied at the Tunceli StateHospital for the period between July 2013 and January 2015. Data was collected from the database of Tunceli State Hospital and analyzed using SPSS 15.00. Results: A total of 3640 patients tested for HBsAg were included in this study. 154 patients (4.23 % were found HBsAg positive. 33 (0.94 % of 3489 patients were found anti-HCV positive and 1829 (46.41% of 3941 patients were found anti-HBs positive. Conclusions:While an earlier study had produced similar results to the current study in Tunceli province, the seroprevalence of HBV and HCV are still high. Therefore, community awareness should be raised through health education about transmission and prevention of HBVand HCV infections.

  13. Survey of the level of anti-HBs antibody titer in vaccinated Iranian general dentists.

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    Alavian, Seyed-Moayed; Izadi, Morteza; Zare, Ali-Akbar; Lankarani, Maryam Moghani; Assari, Shervin; Vardi, Mohammad-Mahdi

    2008-01-01

    Hepatitis B is an infectious disease to which dentists are susceptible. The main aim of this study was to determine the level of antibody titer and immunity in vaccinated Iranian general dentists. A total of 861 general dentists were invited to participate in this study; 598 persons who could recall their history of vaccination and consented to have blood samples taken were recruited. Demographic and work-related data were recorded, and anti-Hepatitis B surface antigen (anti-HBs-Ag) evaluations were measured using the enzyme-linked immunosorbent assay (ELISA). Of the 598 participants, 35 (5.9%) were nonimmune (anti-HBs anti-HBs = 10-99 IU/l), and 462 (77.3%) were completely immune (anti-HBs > or =100 IU/l). Only 218 (36.5%) of the dentists knew their HBs antibody titer. Fourteen (2.3%) persons reported receiving one dose and 65 (10.9%) had received two doses. The number of those who had received the three recommended doses totaled 519 (86.8%), 491 (82.1%) of them receiving their vaccine on schedule. Age, city, pack-years of smoking, years of smoking, and the interval between the last vaccination and the commencement of the study had a significant relationship to the antibody titer level, whereas sex, marital status, place of practice, smoking, and vaccination schedule were not related. Only 36.5% of the general dentists had checked their antibody titer. We, therefore, recommend that dentists, as a potential high-risk group, should know their level of anti-HBs antibody titer so that those who require revaccination can get treatment.

  14. Research On Athletes in 2010 HBsAg,Anti-HBs Survey Guangxi%2010年广西运动员HBsAg、抗-HBs现状调查

    Institute of Scientific and Technical Information of China (English)

    蒋琳; 赵丽

    2011-01-01

    对2010年广西运动员乙型肝炎表面抗原(HBsAg)、表面抗体(抗-HBs)、分布特点以及发病率进行调查分析.结果表明:广西现役运动员抗-HBs阳性率高达48.1396,略高于1997年;HBsAg阳性率大幅降低.

  15. Mutations in the S gene and in the overlapping reverse transcriptase region in chronic hepatitis B Chinese patients with coexistence of HBsAg and anti-HBs

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    Feng Ding

    2016-02-01

    Full Text Available Abstract Background The mechanism underlying the coexistence of hepatitis B surface antigen and antibodies to HBsAg in chronic hepatitis B patients remains unknown. Aims This research aimed to determine the clinical and virological features of the rare pattern. Methods A total of 32 chronic hepatitis B patients infected by HBV genotype C were included: 15 carrying both HBsAg and anti-HBs (group I and 17 solely positive for HBsAg (group II. S gene and reverse transcriptase region sequences were amplified, sequenced and compared with the reference sequences. Results The amino acid variability within major hydrophilic region, especially the “a” determinant region, and within reverse transcriptase for regions overlapping the major hydrophilic region in group I is significantly higher than those in group II. Mutation sI126S/T within the “a” determinant was the most frequent change, and only patients from group I had the sQ129R, sG130N, sF134I, sG145R amino acid changes, which are known to alter immunogenicity. Conclusions In chronic patients, the concurrent HBsAg/anti-HBs serological profile is associated with an increased aa variability in several key areas of HBV genome. Additional research on these genetic mutants are needed to clarify their biological significance for viral persistence.

  16. Acute hepatitis B virus infection with simultaneous high HBsAg and high anti-HBs signals in a previously HBV vaccinated HIV-1 positive patient.

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    van Dommelen, Laura; Verbon, Annelies; van Doorn, H Rogier; Goossens, Valère J

    2010-03-01

    We present a case of a clinical manifest hepatitis B virus infection and a potentially misleading HBV serological profile in an HIV-1 positive patient despite previous HBV vaccination. The patient presented with an acute hepatitis B and there was no indication of chronic HBV infection or the presence of a mutation in the 'a' determinant. Remarkably, simultaneously with high HBV surface antigen and HBV viral load, high anti-HBs antibodies were present. If, due to previous HBV vaccination only anti-HBs was tested in this patient, the result of the high anti-HBs antibodies could be very misleading and offering a false sense of security. Our findings contribute to the ongoing discussion on how to assess HBV specific immunological memory and determining the role of HBV booster vaccinations in immunocompromised individuals.

  17. [Seroprevalence of HBs Ag and of anti-HCV antibodies among HIV infected people in N'Djamena, Chad].

    Science.gov (United States)

    Bessimbaye, N; Moussa, A M; Mbanga, D; Tidjani, A; Mahamat, S O; Ngawara, M Nahor; Ngarnayal, G; Fissou, H Y; Sangare, L; Ndoutamia, G; Barro, N

    2014-12-01

    This is a prospective study conducted as part of a voluntary testing for HBV, HCV and HIV. The aim of the study is to determine the seroprevalence of HBs Ag and anti-HCV antibodies among HIV infected people and a control group of HIV negative people. HIV prevalence among newly diagnosed volunteers is 9.1%. The overall seroprevalence of HBs Ag and anti-HCV antibodies is respectively 13.5% and 2.0%. The seroprevalence of HBs Ag and anti-HCVantibodies in the control group (HIV-negative) is respectively 12.2% and 2%. The seroprevalence of HBs Ag and anti-HCV antibodies among HIV infected people (old and new) is respectively 16.1% and 1%.This study, the first one conducted in Chad, has allowed us to know the seroprevalence of HBs Ag and anti-HCV antibodies among HIV infected people.

  18. Presence of maternal anti-HBs antibodies does not influence hepatitis B vaccine response in Brazilian neonates.

    Science.gov (United States)

    Junqueira, Ana Luiza N; Tavares, Viviane R; Martins, Regina M B; Frauzino, Kamilla V; Silva, Agabo M da Costa e; Rodrigues, Izolina M X; Minamisava, Ruth; Teles, Sheila A

    2011-02-01

    Recently, it was suggested that maternal hepatitis B surface antigen antibodies (anti-HBs) acquired transplacentally could play a negative role in newborn infants' immune response to the hepatitis B vaccine. We compared the hepatitis B virus (HBV) vaccine response in infants born to mothers previously vaccinated against HBV (n = 91) to infants born to mothers who were not previously vaccinated (n = 221). All newborn infants received three intramuscular doses (10 μg) of HBV vaccine (Butang®) at 0,1 and six months. The first dose was administered at the maternity hospital within 12 h of birth. The geometric mean titres of anti-HBs were not different among newborn infants born to mothers who were anti-HBs-negative (492.7 mIU/mL) and anti-HBs-positive (578.7 mIU/mL) (p = 0.38). Eight infants did not respond to the HBV vaccine. Of them, six were born to anti-HBs-negative mothers and two were born to mothers with anti-HBs titres less than 50 mlU/mL. Despite the mother's anti-HBs-positive status, our data show a good immunogenicity of the Brazilian HBV recombinant vaccine in neonates.

  19. Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix

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    Vázquez-Corzo Sara

    2003-01-01

    Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.

  20. [Requirement of standardizing anti-HBs assay methods in Japan for HBV infection-preventing strategy--discrepancy of anti-HBs measurements among three different kits widely used in Japan].

    Science.gov (United States)

    Ogata, Norio

    2006-09-01

    The strategy to eliminate hepatitis B virus (HBV) infection by administrating an HB vaccine is changing worldwide; however, this is not the case in Japan. An important concern about the HBV infection-preventing strategy in Japan may be that the assay methods for the antibody to hepatitis B surface antigen (anti-HBs) are not standardized. The minimum protective anti-HBs titer against HBV infection has been established as 10 mIU/ml by World Health Organization (WHO) -standardized assay methods worldwide, but that is still determined as a "positive" test result by the passive hemagglutination (PHA) method in Japan. We compared anti-HBs measurements in given samples among PHA(Mycell II, Institute of Immunology), chemiluminescent enzyme immunoassay (CLEIA) (Lumipulse, Fujirebio), and chemiluminescent immunoassay (CLIA) (Architect, Abbott), all of which are currently in wide use in Japan. First, anti-HBs measurements in serum from individuals who received a yeast-derived recombinant HB vaccine composed of the major surface protein of either subtype adr or subtype ayw were compared. The results clearly showed that in subtype adr-vaccinees CLIA underestimated the anti-HBs amount compared with CLEIA and PHA, but in ayw-vaccinees, the discordance in the measurements among the three kits was not prominent. Second, anti-HBs measurements in standard or calibration solutions of each assay kit were compared. Surprisingly, CLEIA showed higher measurements in all three kit-associated standard or calibration solutions than CLIA. Thus, the anti-HBs titer of 10 mIU/ml is difficult to introduce in Japan as the minimum protective level against HBV infection. Efforts to standardize anti-HBs assay methods are expected to share international evidence about the HBV infection-preventing strategy.

  1. DETECCIÓN DE ANTICUERPOS ANTI-Leishmania (Trypanosomatidae EN POBLACIONES CANINAS DEL DEPARTAMENTO DE SUCRE, COLOMBIA

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    Luís Enrique Paternina Tuirán

    2015-11-01

    Full Text Available RESUMENEl objetivo de la investigación fue determinar la prevalencia de anticuerpos anti-Leishmania en perros del departamento de Sucre, Colombia. Se analizaron 122 perros del área rural de los municipios de Ovejas, Sampués y Sincelejo, mediante la técnica de inmunofluorescencia indirecta (IFI. Anticuerpos anti-Leishmania fueron detectados en el 69,6 % (IC95 % = 61,1 – 78,2 de la población canina estudiada. En el municipio de Sincelejo la seropositividad fue del 72 % (IC95 % = 61,1 – 82,8, en el municipio de Sampués del 68,4 % (IC95 % = 52,3 – 84,5 y en el municipio de Ovejas del 55,5 % (IC95 % = 21,2 – 86,3. Los animales seropositivos fueron principalmente caninos jóvenes y adultos. El 17,6 % de los perros seropositivos mostró signos clínicos compatibles con leishmaniasis canina, entre los cuales la onicogrifosis, alopecia y caquexia fueron los más comunes. La alta frecuencia de caninos con anticuerpos a Leishmania detectada en el presente estudio, pone de manifiesto la hiperendemicidad de la leishmaniasis canina en esta zona del país, así como el alto riesgo de brotes epidémicos de la enfermedad.ABSTRACTThe aim of this survey was to determine prevalence of antibodies against Leishmania in dogs from Sucre, Colombia. We analyzed 122 dogs in rural areas from municipalities of Sampués, Ovejas and Sincelejo, by indirect immunofluorescence (IFA. Anti-Leishmania antibodies were detected in 69.6 % (95 % CI = 61.1 to 78.2 of the selected canine population. In the municipality of Sincelejo, Sampues and Ovejas, the seropositivities were 72 % (95 % CI = 61.1 to 82.8, 68.4 % (95 % CI = 52.3 to 84.5 and 55.5 % (95 % CI = 21.2 to 86.3 respectively. Seropositive animals were mainly young and adult dogs. The 17.6 % of seropositive dogs showed clinical signs compatible with canine leishmaniasis; onychogryphosis, alopecia and cachexia were the most common ones. The detected high frequency of canines with antibodies against Leishmania in the

  2. HBsAg阳性人群家庭成员中抗-HBs水平观察与研究%Observation and research of anti-HBs level in family members of HBsAg positive crowd

    Institute of Scientific and Technical Information of China (English)

    江金伦; 陈静; 竺稽定

    2011-01-01

    Objective;To understand the anti - HBs level change before and after immunization through the quantitative detection of anti - HBs in negative members aged under 15 years old among the HBsAg positive crowd and provide scientific basis for immune prevention and control on those who have close contact with chronic HBV infected people, as well as adult HB vaccine immunity. Methods: Microparticle enzyme immunoassay ( MELA) was adopted. Results: 30 HBsAg negative members under 15 years old from HBsAg positive crowd of 2 villages were conducted this detection. The result showed that before immunization, 8 cases were 100 mlU/ml, accounting for 20.0% and average GMT is 112.48 mlU/ml; while after immunization, 1 case was 100 mlU/ml, accounting for 96.1% and average GMT is 712.51 miU/ml. Conclusion; Anti - HBs level increased obviously after HB vaccine inoculation, which can be taken as an effective way to prevent and control HBV infection for those family members of HBsAg positive crowd.%目的:通过对HBsAg阳性人群家庭成员中大于15岁HBsAg阴性成员进行抗-HBs水平的定量检测,了解其免疫前后抗-HBs水平的变化,为慢性HBV感染者人群密切接触者免疫预防与控制和成人乙肝疫苗免疫策略提供重要的科学依据.方法:采用微粒子酶免疫分析技术(MEIA).结果:2010年对我市的两个自然村HBsAg阳性人群家庭成员中大于15岁HBsAg阴性成员共30人进行了抗-HBs水平的定量检测,其中免疫前<10 mIU/ml 8人,占26.7%a;10 mIU/nd-50 mIU/ml9人,占30.0%;50 mIU/ml-100 mIU/ml 7人,占23.3%;>100 mIU/ml 6人,占20.0%;平均GMT为112.48 mIU/ml;免疫后<10 mIU/ml 1人,占3.3%;>100 mIU/nil 29人,占96.7%;平均GMT为712.51 mIU/ml.结论:经过乙肝疫苗的接种后,抗-HBs水平都呈明显的上升,因而HBsAg阳性人群家庭成员可通过接种乙肝疫苗来预防与控制HBsAg阳性人群通过水平或性传播等方式造成家庭成员的HBV的感染.

  3. Reactivation of viral replication in anti-HBe positive chronic HBsAg carriers

    DEFF Research Database (Denmark)

    Krogsgaard, K; Aldershvile, J; Kryger, Peter

    1990-01-01

    Reactivation of hepatitis B virus replication was investigated in an unselected group of 44 HBV DNA negative, anti-HBe positive chronic HBsAg carriers. Twenty-five patients (54%) were intravenous drug addicts and 7 (16%) were male homosexuals. Sixteen patients had evidence of delta infection...... and five of the seven male homosexuals had human immunodeficiency virus infection. The patients were followed for 1 to 180 months (median, 24 months) while HBV DNA negative, anti-HBe positive. Reactivation, defined as reappearance of HBV DNA or HBeAg, or both, was detected in six patients corresponding...

  4. Vasculitis asociadas a anticuerpos anti-citoplasma de neutrófilos: Clínica y tratamiento

    OpenAIRE

    María Virginia Paolini; Juan Pablo Ruffino; Diego S. Fernández Romero

    2013-01-01

    Las vasculitis asociadas a anticuerpos anti-citoplasma de neutrófilos (ANCA) comprenden a un grupo de enfermedades caracterizadas por la inflamación de la pared de pequeños vasos. Analizamos las características epidemiológicas y clínicas en una serie de 47 pacientes: 23 (49%) granulomatosis de Wegener (GW), 15 (32%) poliangeítis microscópica (PAM) y nueve (19%) vasculitis limitada al riñón (VLR). La edad media al inicio de los síntomas fue de 50.7 ± 14.9 años. La manifestación clínica más fre...

  5. Association between anti-HBc positivity and hepatocellular carcinoma in HBsAg-negative subjects with chronic liver disease

    Science.gov (United States)

    Coppola, Nicola; Onorato, Lorenzo; Sagnelli, Caterina; Sagnelli, Evangelista; Angelillo, Italo F.

    2016-01-01

    Abstract A meta-analysis was performed to ascertain to what extent hepatitis B surface antigen (HBsAg)-negative/anti-hepatitis B core (anti-HBc)-positive subjects with chronic liver disease are at a higher risk of developing hepatocellular carcinoma (HCC) than the anti-HBc-negative. All studies included had to fulfill the following characteristics and inclusion criteria: they investigated the relationship between HBsAg-negative/anti-HBc-positive serology and the occurrence of HCC, whether a case–control or cohort study, they provided relative risk (RR) or odds ratios (ORs) and 95% confidence intervals (CIs), were available as a full text written in English, and were published and indexed up to April 2015. Twenty-six original studies met the inclusion criteria, allowing a meta-analysis on 44,553 patients. The risk of HCC among the 9986 anti-HBc-positive subjects was 67% higher than in the 34,567 anti-HBc-negative (95% CI = 1.44–1.95, P HCV and non-HCV) were considered. The risk of HCC was significantly higher in the 651 anti-HBs/anti-HBc-positive patients (RR = 1.36; 95% CI = 1.17–1.58, P = 0.03) and in the 595 anti-HBs-negative/anti-HBc-positive subjects (RR = 2.15; 95% CI = 1.58–2.92, P < 0.0001) than in the 1242 anti-HBs/anti-HBc negative. However, the RR from 8 studies indicated that the risk of HCC was 35% lower among the anti-HBs/anti-HBc-positive subjects compared to the anti-HBs-negative/anti-HBc-positive (RR = 0.65; 95% CI = 0.52–0.8, P < 0.0001). This meta-analysis shows that in HBsAg-negative subjects with chronic liver disease, anti-HBc positivity is strongly associated with the presence of HCC, an association observed in all subgroups according to the stage of the disease, etiology, and ethnicity. PMID:27472708

  6. Anticuerpos ANTI-HTLV-I/II en una población del Amazonas colombiano

    Directory of Open Access Journals (Sweden)

    Abraham Blank

    2004-03-01

    Full Text Available

    En Colombia ya son varias las regiones donde se ha informado la presencia de la infección por los retrovirus linfotrópicos humanos tipos I y II (HTLV-I y HTLV-II. La principal región endémica para HTLV-I es el sur de la costa pacífica. El HTLV-II se ha encontrado en grupos indígenas que viven en diferentes regiones rurales como en la Guajira, la Orinoquía, en la costa pacífica y en una familia en Cali (urbana. Hasta el momento los estudios hechos en el Amazonas colombiano no habían confirmado la presencia de estos retrovirus. Se pretende estudiar la presencia de anticuerpos contra HTLV-I y/o HTLV-II utilizando una prueba comercial de Western blot que confirma y diferencia la infección por cada uno de los dos retrovirus mencionados, en caso de encontrar pruebas reactivas en pruebas de tamización.

    Luego de aceptar la participación en este estudio, se buscó la presencia de anticuerpos contra los retrovirus HTLV-I/II con pruebas de tamización en un total de 401 muestras de suero de personas que atendieron al Hospital de Leticia y a la consulta médica en Puerto Nariño. La muestra correspondió a 238 personas de raza mestiza y 163 de origen amerindio. Se realizaron pruebas de ELISA y/o aglutinación de partículas. Las muestras con pruebas de tamización reactivas, se sometieron a un estudio confirmatorio de la infección mediante el estuche comercial de Western blot Problot 2.4 de GeneLabs. En una mujer amerindia se demostró anticuerpos contra el HTLV-II, enun hombre mestizo contra el HTLV-I y en otro hombre mestizo contra el HTLV-II. No había enfermedad asociada en las personas estudiadas.

    La prevalencia de infección por los HTLV en la muestra estudiada fue 0.75%. Aunque este porcentaje no es muy elevado, debe

  7. Reactivation of viral replication in anti-HBe positive chronic HBsAg carriers

    DEFF Research Database (Denmark)

    Krogsgaard, K; Aldershvile, J; Kryger, Peter

    1990-01-01

    Reactivation of hepatitis B virus replication was investigated in an unselected group of 44 HBV DNA negative, anti-HBe positive chronic HBsAg carriers. Twenty-five patients (54%) were intravenous drug addicts and 7 (16%) were male homosexuals. Sixteen patients had evidence of delta infection...... and five of the seven male homosexuals had human immunodeficiency virus infection. The patients were followed for 1 to 180 months (median, 24 months) while HBV DNA negative, anti-HBe positive. Reactivation, defined as reappearance of HBV DNA or HBeAg, or both, was detected in six patients corresponding...... to an annual reactivation rate of 5%. Reactivation in four patients was detected by reversion to HBV DNA positivity only, whereas HBeAg/anti-HBe status remained unchanged. Two patients became both HBV DNA and HBeAg positive. None of the patients developed hepatitis-like symptoms and transaminase elevation...

  8. Study of IgG subclass profiles of anti-HBs in populations with different HBV infection status.

    Science.gov (United States)

    Tsai, Tzu-Hsin; Huang, Chien-Fu; Wei, James Cheng-Chung; Ho, Mei-Shang; Wang, Lina; Tsai, Wei-Yu; Lin, Chien-Chou; Xu, Fang-Ling; Yang, Chi-Chiang

    2006-01-01

    To study IgG-specific subclasses of hepatitis B virus (HBV) surface antigen (anti-HBs), in different populations in Taiwan, a comparison was made between 104 chronic carriers (60 male and 44 female) and 439 recovered individuals (247 male and 192 female). Biochemical analyses of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were also performed. Among the 104 chronic carriers, 21 patients had abnormal ALT and AST levels (> 25 IU/ml). When comparing the patients with abnormal ALT and AST levels to chronic carriers with normal ALT and AST levels, no statistical difference was observed for anti-HBs levels (p > 0.05). The IgG subclass pattern of the relative anti-HBs IgG subclass titers was IgG1 > IgG3 = IgG4 in both chronic carriers and recovered individuals (p anti-HBs antibody after HBV infection, in either chronic carriers or in HBV-cured individuals. This finding is partly inconsistent with data reported from other group who suggested in individuals naturally infected, the anti-HBs IgG consists mainly of IgG3 and IgG1. In contrast to that of our previous studies of anti-HBe and anti-HBc, the mean OD values of anti-HBs total IgG, and all IgG subclasses except for IgG2, of either males or females, were significantly higher in recovered individuals than in chronic carriers, while the mean OD values of anti-HBe and anti-HBc were significantly higher in chronic carriers than in recovered individuals (P anti-HBs in chronic carriers was not changed with liver inflammation and was independent of sex and age, except in individuals with abnormal ALT and AST for whom anti-HBs IgG1 was not significantly higher than IgG3 (p > 0.05), in spite of that whose mean O.D. value is higher.

  9. Short-term response to a booster dose of hepatitis B vaccine in anti-HBs negative adolescents who had received primary vaccination 16 years ago.

    Science.gov (United States)

    Wang, Li-Yu; Lin, Hans Hsienhong

    2007-10-10

    We conducted a revaccination study to investigate the short-term response to booster hepatitis B (HB) vaccination in seronegative adolescents who had received primary infantile HB vaccination. A booster dose of recombinant HB vaccine was administered to 395 adolescents 15-18 years of age whose serum titers of antibody against hepatitis B surface antigen (HBsAg) (anti-HBs) were anti-HBs seropositivity (postbooster titers> or =10 mIU/mL). As compared with adolescents who had undetectable prebooster anti-HBs titers (anti-HBs titers than for those with prebooster titers of 0.1-0.9 and 1.0-9.9 mIU/mL. Our observations indicate that a booster dose of HB vaccine maybe unable to induce sufficient immunological response in adolescents who had undetectable residual anti-HBs titers.

  10. Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix

    OpenAIRE

    Vázquez-Corzo Sara; Trejo-Becerril Catalina; Cruz-Valdez Aurelio; Hernández-Nevarez Pilar; Esquivel-Guadarrama Fernando Roger; Gutiérrez-Xicotencatl Ma de Lourdes

    2003-01-01

    Objetivo. Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. Material y métodos. Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELI...

  11. Analysis on S-gene quasispecies sequence of patients with simultaneously positive HBsAg and Anti-HBs%HBsAg与抗-HBs同时阳性者HBV S基因准种分析

    Institute of Scientific and Technical Information of China (English)

    张硕; 沈立萍; 张爽; 王锋; 张勇; 毕胜利

    2014-01-01

    目的 分析HBsAg与抗-HBs同时阳性者体内乙型肝炎病毒(HBV)S基因准种分布及基因突变情况.方法 收集3例HBsAg与抗_HBs同时阳性者的血清和基本信息,提取HBV DNA,用PCR法扩增HBV S基因,将S基因克隆至载体,测序后通过软件分析准种序列差异、计算遗传距离分析进化选择压力.结果 1号样本感染的HBV为B2型,2号和3号为C5型;准种遗传进化分析发现1号同时存在两种优势病毒株,2号和3号只有一种优势病毒株;三者的信息熵水平无统计学差异;选择压力分析显示1号样本病毒种群的Ka<Ks,ω=0.58,受到纯化选择压力;2号发生AAF134S、AAS143P置换突变,3号发生AAT125A置换突变和ntG494A、ntC500A无义突变.结论 HBsAg与抗_HBs同时阳性者S基因存在复杂的准种多样性;在准种中发现少量a抗原决定簇关键位点突变毒株,通过准种分析研究能真实地体现HBV生存状态.%Objective To analyze distribution and mutation of HBV quasispecies heterogeneity in patients with concurrent existence of HBsAg and HBsAb in serum.Methods Three HBsAg and Anti-HBs simultaneously positive carriers who received whole course timely vaccination were analyzed.The HBV DNA was extracted from the serum,S gene was amplified by PCR assay,then cloned and sequenced.Entropy,genetic distances,nosynonymous (Ka) and synonymous (Ks) substitution rates and molecular phylogenetics,were evaluated for evolutionary analysis.Results The genotype of HBV in first sample was B2 and in other samples was C5.Two kinds of predominant quasispecies were observed in first sample base on genetic distances and quasispecies distribution.One predominant quasispecies were observed in other samples.Entropy values of three samples had no significant difference.The ratios of Ka/Ks of first sample were lower than those of the other samples with ω =0.58 (Ka < Ks).The quasispecties in first sample were under negative (purifying) selection pressure.The point

  12. Anti-HIV seropositivity was related to HBsAg seropositivity among injecting drug users in Taiwan.

    Science.gov (United States)

    Hsieh, Meng-Hsuan; Hsieh, Ming-Yen; Huang, Chung-Feng; Yeh, Ming-Lun; Wang, Shu-Chi; Yang, Jeng-Fu; Chang, Ko; Lin, Wei-Ru; Lin, Chun-Yu; Chen, Tun-Chieh; Huang, Jee-Fu; Dai, Chia-Yen; Tsai, Jih-Jin; Chuang, Wan-Long; Yu, Ming-Lung

    2016-02-01

    In Taiwan, the number of new cases of human immunodeficiency virus (HIV) infection via drug injection has been increasing since 2003. Due to HIV and hepatitis B virus (HBV) having similar transmission routes, HBV and HIV infections among injecting drug users (IDUs) has become an important public health issue. The aim of this study was explore the prevalence of HBV infection among IDUs with and without HIV infection, and examine whether HIV infection is associated with HBV infection among IDUs in Southern Taiwan. We enrolled 566 IDUs, including 87 anti-HBV positive IDUs and 479 anti-HBV negative IDUs, and also analyzed the results of liver function tests, HBV DNA, anti-HIV, HIV RNA, and CD4 cell count. The results showed that the prevalence of HBV infection among IDUs was 15.4%. The prevalence of hepatitis B surface antigen (HBsAg) was higher among individuals born before 1985 (15.9% vs. 4.0%), but this was not significant. Anti-HIV seropositivity was related to HBsAg seropositivity [odds ratio (OR) = 2.47, 95% confidence interval = 1.26-4.82, p = 0.008). Anti-HCV and anti-HIV were risk factors for abnormal alanine aminotransferase (ALT; OR = 2.11, 95% confidence interval = 1.005-4.42, p = 0.048 and OR = 1.47, 95% confidence interval = 1.02-2.10, p = 0.04, respectively), and HBsAg was not a factor related to abnormal ALT. In conclusion, the prevalence of HBV infection was similar in the general population and in IDUs, and due to anti-HIV seropositivity being significantly related to HBsAg seropositivity, HBV infection among IDUs is still important. We suggest that for IDUs, HBsAg should be monitored closely.

  13. HLA and response to booster hepatitis B vaccination in anti-HBs-seronegative adolescents who had received primary infantile vaccination.

    Science.gov (United States)

    Lin, Hans Hsienhong; Liao, Huei-Wen Chang; Lin, Sheng-Kai; Wang, Li-Yu

    2008-06-25

    To explore contemporarily genetic and non-genetic determinants of long-term immunological memory to hepatitis B (HB) vaccination, we conducted a case-control study nested in an adolescent cohort of booster recipients who had received primary infantile HB vaccination but with residual anti-HBs titers anti-HBs levels, the absences of HLA-A*02 and -DRB1*08, simply expressed as A*02(-) and -DRB1*08(-), and the presence of B*15 were significantly associated with elevated risks of non-response (post-booster anti-HBs titers0.05), and 1.0, respectively. Recent cigarette smoking and/or betel-quid chewing was associated with a 12-fold risk of non-response to booster vaccination. Further comparisons between responders and adolescents who had undetectable post-booster anti-HBs titers (<0.1 mIU/mL) demonstrated similar results. Our results indicated that response to booster HB vaccination as well as long-term immunological responses to HB vaccination are closely related with host genetic factors, and probably modified by recent substance use.

  14. Comparison of hepatitis B, core, HBc, and hepatitis B antibody, anti HBs, in a presumed low risk donor population.

    Science.gov (United States)

    Heck, Ellen; Cavanagh, H Dwight

    2014-09-01

    Donors screened by medical social history interview negative for high risk behavior or communicable disease history, but subsequently exhibiting reactive serological markers, emphasize importance of duel safe guarding factors for determining donor suitability. This report examines a relationship between two immunoabsorption assay tests, hepatitis B core (HBc) antibody, a required food and drug administration (FDA) test, and hepatitis B antibody (anti HBs), non-required test. Reactive serology results, 129 cases, 3,581 donors (2008-2012) for HBc as the only initially positive serological marker were subjected to anti HBs testing in this history pre-screened donor population. Enzyme linked immunoabsorption assay kits hepatitis B, core and antibody, were used in this study. All samples were initially tested for human immunodeficiency virus, hepatitis B, and hepatitis C, utilizing nucleic acid testing and antigen antibody immunoabsorption assay. Testing was performed by a FDA-registered CLEA-certified reference laboratory. Samples were deceased donor blood samples and a limited number of pre-mortem samples, separated, stored and analyzed according to manufacturer recommendation and FDA regulations. 129 reactive HBc only samples, were subsequently tested for anti HBs. Of these 129, 94 were found to be reactive for anti HBs. This represented 72 % of samples tested for antibody, a higher percentage than anticipated for a medical history negative, low risk population.

  15. Detection of Hepatitis B Virus (HBV) Genotype E Carried—Even in the Presence of High Titers of Anti-HBs Antibodies—by an Argentinean Patient of African Descent Who Had Received Vaccination against HBV

    Science.gov (United States)

    Mathet, Verónica L.; Cuestas, María L.; Ruiz, Vanesa; Minassian, María L.; Rivero, Cintia; Trinks, Julieta; Daleoso, Graciela; León, Liliana M.; Sala, Andrea; Libellara, Beatriz; Corach, Daniel; Oubiña, José R.

    2006-01-01

    Genotype E hepatitis B virus (HBV) was detected in two Argentine sisters exhibiting an African mitochondrial lineage. One of them (who had been vaccinated against HBV) exhibited anti-HBs cocirculating antibodies without HBsAg escape mutants, while her unvaccinated sister showed a D144A HBsAg escape mutant without anti-HBs antibodies. Both sisters carried an unusual L209V substitution within HBsAg. PMID:16954295

  16. Vasculitis asociadas a anticuerpos anti-citoplasma de neutrófilos: Clínica y tratamiento

    Directory of Open Access Journals (Sweden)

    María Virginia Paolini

    2013-04-01

    Full Text Available Las vasculitis asociadas a anticuerpos anti-citoplasma de neutrófilos (ANCA comprenden a un grupo de enfermedades caracterizadas por la inflamación de la pared de pequeños vasos. Analizamos las características epidemiológicas y clínicas en una serie de 47 pacientes: 23 (49% granulomatosis de Wegener (GW, 15 (32% poliangeítis microscópica (PAM y nueve (19% vasculitis limitada al riñón (VLR. La edad media al inicio de los síntomas fue de 50.7 ± 14.9 años. La manifestación clínica más frecuente fue el compromiso renal en 41 (87% pacientes, seguido por el pulmonar en 26 (55% y el otorrinolaringológico en 17 (36%. En 26 (55% se asoció compromiso renal y pulmonar. La forma clínica más frecuente fue la generalizada en 23 (49%, seguida por la grave en 18 (38%. El 89% presentaron determinaciones de ANCA positivas. Cuatro (8% no recibieron tratamiento inmunosupresor de inicio. De los 43 que recibieron tratamiento de inicio, 29 (67% tuvieron remisión completa, con un tiempo de remisión promedio de 35.3 meses. Once (26% presentaron recaídas, diez (91% recaídas mayores y uno (9% menor. Doce (28% fallecieron, siete en forma temprana y cinco durante la evolución de la enfermedad. Quince (31% evolucionaron a insuficiencia renal crónica. Los 26 pacientes en seguimiento tuvieron respuesta al tratamiento y 20 (77% de ellos estaban en remisión al finalizar el estudio. Las vasculitis asociadas a ANCA continúan siendo enfermedades de alta morbilidad y mortalidad, a pesar de las mejorías logradas con los tratamientos inmunosupresores.

  17. Serum Anti-Hbs-Ag in Stable Hemodialysis Patients and its Relationship with Various Demographic and Biochemical Data

    Directory of Open Access Journals (Sweden)

    Azar BARADARAN

    2010-12-01

    Full Text Available Introduction: To evaluate the relationship between various biochemical, nutritional and demographic factors with immune response to hepatitis B vaccine in maintenance hemodialysis (HD patients. Material and Methods: A retro-prospective study was carried out on 68 patients undergoing maintenance hemodialysis .Patients were vaccinated against hepatitis B virus with an intramuscular hepatitis B vaccination schedule, 40 micrograms at 0, 1, and 6 months. We also selected 32 age matched normal healthy persons who had vaccinated against hepatitis B previously to compare the antibody production with HD patients. Results: The value of serum antibody level against hepatitis B surface antigen ( HBs in hemodialisis patients and healthy persons were 35±55(median=5.5 and 135±71 (median=175 mIU/ml respectively. There was a significant deference between mean serum antibody level against HBs antigen of hemodialysis patients and normal subjects (p<0.001, there were not any significant differences of antibody production against HBs antigen between males and females or diabetic and non diabetics. There were no correlation between serum antibody level against HBs-Ag and serum albumin and also with body mass index. There were not significant correlation between anti-HBs antibody level and age, amounts of hemodialysis, duration of dialysis, dialysis adequacy, serum ferritin level and serum lipids. There were not also significant correlation between anti-HBs antibody level and serum parathormone, calcium, phosphorus, serum hemoglobin and hematocrit level. Conclusion: In this study, there was not significant correlation between serum antibody level against hepatitis B surface antigen and various nutritional and demographic factors of patients under regular hemodialysis.

  18. Late hepatitis B virus reactivation after lamivudine prophylaxis interruption in an anti-HBs-positive and anti-HBc-negative patient treated with rituximab-containing therapy.

    Science.gov (United States)

    Ceccarelli, Laura; Salpini, Romina; Sarmati, Loredana; Svicher, Valentina; Bertoli, Ada; Sordillo, Pasquale; Ricciardi, Alessandra; Perno, Carlo Federico; Andreoni, Massimo; Sarrecchia, Cesare

    2012-08-01

    We describe a case of an anti-HBs-positive patient who experienced hepatitis B reactivation 18 months after the discontinuation of rituximab and after 12 months of lamivudine prophylaxis. The patient carried a hepatitis B genotype D virus harbouring a single immune escape mutation, sT118K. No consensus guidelines regarding the optimal length of treatment or the best elective drug have been defined for antiviral prophylaxis for HBsAg-negative, anti-HBc- and/or anti-HBs-positive patients undergoing immunosuppressive treatment. Screening based on HBV serological markers and HBV DNA testing is a critical issue to recognise hepatitis B reactivation as early as possible. Furthermore, it is of outstanding importance to identify alternative markers (e.g. cccDNA, HBV core related antigen, etc.), that could be predictive of HBV reactivation.

  19. Primary research of hepatitis B virus infected patients serum with HBsAg and anti-HBs double positive%乙型肝炎病毒表面抗原与表面抗体同时阳性的血清学模式初步研究

    Institute of Scientific and Technical Information of China (English)

    王珍光; 邓芝云; 郭建巍; 马骢; 荣扬; 刘敏

    2011-01-01

    目的 分析乙型肝炎病毒感染者乙型肝炎表面抗原与乙型肝炎表面抗体同时阳性的血清学模式与HBV DNA 的关系,并探究其原因及临床意义.方法 用酶联免疫分析法筛选出HBsAg和抗-HBs同时阳性的标本,用化学发光微粒子免疫分析法确认,用实时荧光定量聚合酶链反应检测其HBV DNA含量.结果 在选取的HBsAg和抗-HBs双阳性的56人中,共出现4 种HBV血清学模式:(1)HBsAg、抗-HBs、抗-Hbe、抗-HBc 阳性模式占42.8%,HBV DNA阳性率41.6%;(2)HBsAg、抗-HBs、HBeAg、抗-HBc阳性模式占30.4%,HBV DNA阳性率70.5%;(3)HBsAg、抗-HBs、HBcAb阳性模式占25%,HBV DNA 阳性率21.4%.(4)HBsAg、抗-HBs、HBeAg 、抗-Hbe、抗-HBc阳性模式占1.8%,HBV DNA阳性率100%.56 例样本中,有27例血清HBV DNA 检测阳性,阳性率为48.2%.结论 HBsAg和抗-HBs 双阳性伴HBeAg阳性者,血清中有较高水平的HBV DNA.HBsAg 和抗-HBs 双阳性并不代表疾病好转.%Objective To analyze of serum with HBsA g and anti HBs double positive in patients with Hepatitis B virus infec tion and discuss the relationship with HBV DNA. Methods to screening HBsAg and anti HBs double positive serum with enzyme linked immunosorbent assay and check the serum HBV markers and HBV DNA by Immunochemiluminescent technology and real time PCR respectively. Results Among the 56 cases with HBsAg and anti-HBs double positive, there were four HBV serology models: (1)HBsAg,anti-HBs, HBeAg and anti-HBc positive, with a positive rate of 42.8 % and HBV DNA positive rate 41.6 %.(2)HBsAg,anti-HBs and anti-HBc positive with a positive rate 30.4% and HBV DNA positive rate 70.5%. (3)HBsAg,anti HBs,anti HBe and anti HBc positive with a positive rate 25% and HBV DNA positive rate 21.4%. (4)HBsAg,anti HBs,HBeAg,anti HBe and anti HBc positive model with a 1.8% positive rate and whole positive of HBV DNA. There were 27 cases in 56 HBsAg and anti HBs double positive samples with a positive rate of 48

  20. Anticuerpos anti 21 hidroxilasa séricos en pacientes con anticuerpos antifracción microsomal: Síndrome poliendocrino autoinmune Seric 21- hydroxilase antibodies in patients with anti-microsomal fraction antibodies: Autoimmune polyendocrine syndrome

    Directory of Open Access Journals (Sweden)

    Silvia Botta

    2007-04-01

    Full Text Available El síndrome poliendocrino autoinmune (SPA es la asociación de enfermedades endocrinas autoinmunes con otros desórdenes autoinmunes no endocrinos. Los tipos 1, 2 y 4 presentan adrenalitis autoinmune, esto indica la presencia de autoanticuerpos, y su marcador serológico específico es el anti 21 hidroxilasa (a21-OH. El SPA tipo 2 es la asociación de adrenalitis, enfermedad tiroidea y/o diabetes mellitus inducidas por autoanticuerpos. Como componentes menores, pueden estar asociados entre otros, vitiligo, alopecia y miastenia. Nuestros objetivos fueron: establecer la prevalencia de a21-OH séricos en pacientes con anticuerpos anti fracción microsomal (AFM positivos, enfermedad tiroidea autoinmune y/o afecciones endocrinas y no endocrinas autoinmunes; diagnosticar formas incompletas de SPA y estudiar individuos con probable riesgo de progresión a un SPA completo. Estudiamos 72 pacientes AFM positivos y 60 sujetos tomados como grupo control, AFM negativos. Hallamos a21-OH elevados en dos pacientes: A= 47 U/ml, hipotiroidismo autoinmune y miastenia; y B= 8.75 U/ml, hipotiroidismo autoinmune y vitiligo; ambos con ausencia de insuficiencia adrenal. La prevalencia de a21-OH encontrada fue del 2.8%. Las pacientes A y B corresponden a un SPA tipo 2 incompleto y latente en relación al componente adrenal. Considerando a los a21-OH marcadores de enfermedad autoinmune latente, el eventual riesgo de evolución hacia la afección clínica sugiere la necesidad de estrechos controles clínicos y bioquímicos periódicos.Autoimmune polyendocrine syndrome (APS is the association of autoimmune endocrine diseases, with other autoimmune nonendocrine disorders. APS types 1, 2 and 4 include autoimmune adrenalitis; this suggests the presence of autoantibodies. A specific serological marker for these is the anti 21- hydroxilase autoantibody (a21-OH. APS type 2 is the association of autoimmune adrenalitis, to autoimmune thyroid disease and/or diabetes mellitus, all

  1. High titers of anti-HBs prevent rituximab-related viral reactivation in resolved hepatitis B patient with non-Hodgkin's lymphoma.

    Science.gov (United States)

    Cho, Yuri; Yu, Su Jong; Cho, Eun Ju; Lee, Jeong-Hoon; Kim, Tae Min; Heo, Dae Seog; Kim, Yoon Jun; Yoon, Jung-Hwan

    2016-06-01

    Rituximab, an anti-CD20 monoclonal antibody, is associated with an increased risk of hepatitis B virus (HBV) reactivation. This study aimed to determine the predictive factors for rituximab-related HBV reactivation in resolved hepatitis B patients, defined as HBsAg-negative, anti-HBc-positive, and undetectable HBV DNA. Among 840 consecutive patients with CD20-positive B-cell lymphoma who received rituximab-based chemotherapy from 2003 through 2014 at Seoul National University Hospital, 732 patients were excluded because either anti-HBc was not assessed or they were HBsAg-seropositive. This retrospective study included 108 resolved hepatitis B patients. During a median 33.5-month follow-up period, eight cases of HBV reactivation occurred only among the patients with low anti-HBs titers (anti-HBs titers were the protective factors for HBV reactivation (hazard ratio [HR], 0.90 and 0.95, respectively). Among those who did not receive antiviral prophylaxis, patients with high baseline anti-HBs (≥100 mIU/ml) experienced significantly lower risk of HBV reactivation (HR, 0.49; P = 0.006) than the patients with low baseline anti-HBs (anti-HBs titer at baseline and antiviral prophylaxis prevented HBV reactivation, suggesting antiviral prophylaxis should be considered according to baseline anti-HBs titer. Meticulous follow-up for ALT and HBV DNA without antiviral prophylaxis might be possible for the patients with high baseline anti-HBs (≥100 mIU/ml).

  2. [Is it necessary to revaccinate against hepatitis B virus when the titer of anti-HBs drops below 10 IU/L?

    Science.gov (United States)

    Polák, Pavel; Husa, Petr; Smejkal, Petr; Kamelander, Jan; Chlupová, Gabriela; Penka, Miroslav

    2016-09-01

    Viral hepatitis B still represents a major epidemiological issue worldwide. After recombinant vaccine administration, a titer of anti-HBs antibodies ≥ 10 IU/L has long been considered to be seroprotective. Persons without this antibody level after complete vaccination are referred to as non-responders. A progressive decline in anti-HBs levels over years is also commonly seen in responders. Recently, there has been increasing evidence that the titer of anti-HBs ≥ 10 IU/L does not provide 100 % protection against infection and clinically manifest illness. Additionally, an important role of cellular immune memory has been demonstrated - without any correlation with anti-HBs titer. Based on current knowledge, there is no need for regular anti-HBs titer testing or booster vaccination in immunocompetent individuals with anti-HBs ≤ 10 IU/L. At present, regular serological testing and, if need be, revaccination to maintain anti-HBs ≥ 10 IU/L are recommended in immunocompromised persons and after liver transplantation.

  3. Inmunodetección de anticuerpos anti-insulina libres en suero y unidos a la molécula de insulina en pacientes insulino-dependientes

    OpenAIRE

    Domínguez Romero, Leidy Yohana

    2014-01-01

    El uso de la terapia insulínica como tratamiento para la Diabetes, basado en la administración de Insulina Exógena (IE) de origen animal –con alta homología a la insulina humana, a nivel de la secuencia proteica, se asoció en sus inicios con la prevalencia de Anticuerpos Anti-Insulina (AAI) [1, 2], relacionados a su vez con alteraciones en la seguridad y eficacia de estas formulaciones [3-6]. Con la aparición de insulinas de origen recombinante –bajo la premisa de la identidad con la secuenci...

  4. 2658份血清标本中anti-HBs、anti-HBe、anti-HBc分布模式及形成机制的探讨%Distribution pattern and formation mechanism of anti-HBs, anti-HBe, anti-HBc in 2658 serum samples

    Institute of Scientific and Technical Information of China (English)

    孔丽清; 刘恩华; 齐雅君; 唱丽敏

    2013-01-01

    Objective:To explore the distribution pattern of antiboby markers during acute and chronic HBV infection and their formation mechanism.Methods:HBV infectious makers were quantified by chemiluminescent microparticle immunoassay (CMIA) in 2658 serum samples of outpatients and hospitalized patients,and the test data of antiboby markers were statistically grouped and analyzed.Results:The distribution rate of single antiHBsIgG was different from that of anti-HBsIgG+anti-HbcIgG,with statistical significance; the comparation of anti-H-BcIgG levels between HBsAg(N)+ anti-HBs(R) group and HBsAg(N)+ anti-HBs(R)+ anti-HBc(R) group had statistical significance (P<0.05; anti-HBe(S/CO) levels between HBsAg(R)+ anti-HBe(R)+ anti-HBc (R) group and HBsAg(R) +HBeAg(R)+ anti-HBc(R) group had statistical significance (P<0.01); anti-HBc(S/CO) levels between HBsAg(R)+ anti-HBe(R)+ anti-HBc(R) group and HBsAg(R) +HBeAg(N)+ anti-HBc(R) group had statistical significanc (P<0.05).Conclusion:Not only can HBV infectious markers be used for diagnosis of acute and chronic HBV infections,but also the stratification,cure-guide and evaluation.%目的:探讨HBV病毒急慢性感染过程中抗体类标志物的分布模式及形成机制.方法:采用化学发光微粒子免疫检测法(CMIA法)定量分析2012年2月10日-6月10日来我院就诊的2658例门诊或住院患者的血清标本中的HBV病毒感染标志物,根据抗原性标志物实验结果将抗体类标志物实验结果的分布模式分组进行统计学分析.结果:发现HBsAg无反应性组(N)内单纯anti-HbsIgG与有反应性分布差异有统计学意义;HBsAg有反应性组anti-HBeIgG+anti-HBcIgG有反应性分布与另两组组间比较差异有统计学意义(P<0.05);anti-HBsIgG、anti-HBcIgG、anti-HBeIgG表达含量在各实验组存在着差异.anti-HBcIgG含量在HBsAg(N)+ anti-HBs(R)组和HBsAg(N)+ anti-HBc(R)组差异有统计学意义(P<0.05);anti-HBe(S/CO)在HB-sAg(R)+ anti-HBe(R)+ anti-HBc(R)组和HBs

  5. PreS deletion mutations of hepatitis B virus in chronically infected patients with simultaneous seropositivity for hepatitis-B surface antigen and anti-HBS antibodies.

    Science.gov (United States)

    Huang, Xiangyan; Qin, Yanghua; Zhang, Peng; Tang, Gusheng; Shi, Qingfen; Xu, Jun; Qi, Falian; Shen, Qian

    2010-01-01

    Hepatitis B surface antigen (HBsAg) and anti-HBs antibodies (anti-HBs) may coexist in certain chronic hepatitis B (CHB) patients. This study was designed to further explore the relationship between this coexistence and hepatitis B Virus (HBV) preS deletions. Sera of 28 patients carrying both HBsAg and anti-HBs (Group I) and those of another 28 HBsAg positive but anti-HBs negative patients (Group II) were collected from CHB patients. Direct sequencing of polymerase chain reaction products or sequencing of clones was applied to both groups to determine sequences of HBV preS and S genes. Genotyping of the S gene indicated that all sampled HBVs were either Genosubtype Ba or Genosubtype Ce. Seven samples in Group I harbored HBV preS deletion mutations. Three of the seven samples showed large deletion mutations in 3' terminus of preS1 and co-existence of the mutant type and the full-length wild type, and the remaining four samples showed deletion mutations in 5' terminus of preS2. All mutant strains were found to be genosubtype Ce. Only two samples in Group I showed G145R/A mutation. Only one sample in Group II contained preS deletion mutation. It is therefore concluded that HBV preS deletion mutations are likely to be related to the coexistence of HBsAg and anti-HBs in CHB patients (P-value = 0.024). Some immune reactions may select for the preS deletion in CHB patients with anti-HBs, the possible marker for immune selection.

  6. 中学生乙型肝炎表面抗原及表面抗体检测分析%Analysis on HBsAg and anti-HBs detection in middle school students

    Institute of Scientific and Technical Information of China (English)

    马雪贞; 郑晓健

    2009-01-01

    Objective To investigete the situation of HBsAg and anti-HBs positive rates of middle school students in Chaoyang district,and to provide the basis for the hepatitis B vaccine immunity in school.Methods Colloid gold fast diagnosis reagent was applied to detect HBsAg and anti-HBs.Results 33 170 students were investigated.The total HBsAg-postitive rate was 5.68%;the total anti-HBs postitive rate was 13.43%.The HBsAg-postitive rate in boys was 5.80%;the HBsAg-postitive rate in girls was 5.54%,the HBsAg-postitive rate from different gender of students was no statistical difference(X2 = 0.93,P>0.05).HBsAg-positive rate in city students(5.22%)was significantly lower than that in rural students(6.14%),and there was statistical difference(X2 = 12.76,P<0.05).HBsAg positive rate of junior high school students(5.29%)was less than that in high school students(6.08%),there was statistical difference(X2 = 9.53,P<0.05).There was no significant difference in anti-HBs postitive rate between the boy (13.6%)and girls(13.2%)(X2 = 0.82,P>0.05).anti-HBs-positive rate(18.18%)in city student was higher than in rural students(8.75%),there was statistical difference(X2 = 633.42,P<0.05);anti-HBs positive rate in high school (15.17%)was up to junior high school(11.69%)(X2 = 85.94,P<0.05).Conclusions HBV infection in middle school students in this area is low popular intensity,however,the hepatits B vaccine basis immunity and strengthening immunity is the key point,especially for the non-infected people.There are statistial difference in the positive rates of HBsAg and anti-HBs between the different area and grade,the vaccine immunity should be set under program.%目的 了解汕头市潮阳区中学生HBsAg和抗-HBs的阳性率情况,为在校内开展有效的免疫干预工作提供科学依据.方法 采用胶体金快速诊断试剂检测HBsAg、抗-HBs.结果 共检测33 170名中学生,HBsAg总阳性率5.68%,抗-HBs总阳性率13.43%.男生HBsAg阳性率(5.80%)与女生HBs

  7. Implications of different serological patterns of HBsAg and anti-HBs coexistence in patients with chronic hepatitis B%慢性乙型肝炎病毒感染者HBsAg和抗-HBs共存的不同血清学模式及其意义分析

    Institute of Scientific and Technical Information of China (English)

    于乐成; 侯金林; 马世武; 王战会; 何长伦; 汪茂荣; 王燕军

    2011-01-01

    Objective To investigate the serological patterns of HBsAg and anti-HBs coexistence in patitents with chronic hepatitis B. Methods 93 patients with HBsAg and anti-HBs coexistence were selected from 3042 patients with chronic HBV infection,and their clinical,serological and virological features were analyzed.Results Serological patterns of HBsAg and anti-HBs coexistence were devided into four types according to the levels of HBsAg and anti-HBs,i.e.,high (H)/low (L) , H/H, UL and UH,which accounted for 43.0%, 14.0%,35.5% and 7.5%,respectively;the positive percentages of HBeAg in patients with the four serological patterns were 55.O% in H/L,38.5% in H/H,18.2% in UL and 14.3% in L/H group;there was a significant difference for the HBV DNA levels as compared between H/L and UL group (P=0.016),but not between H/L and H/H group(P=0.569)or H/H and L/L group (P=0.095). Concluson The lower serum HBsAg level combined with higher anti-HBs level were accompanied with lower rates of HBeAg positivity and lower HBV DNA level in patients with HBsAg and anti-HBs coexistence.%目的 探讨慢性HBV感染者HBsAg和抗-HBs共存的不同血清学模式及其意义.方法 在3042例慢性HBV感染者中筛选出HBsAg和抗-HBs共存患者93例,分析其临床特征、HBsAg和抗-HBs相对滴度模式、HBeAg状态和HBV DNA水平.结果 根据HBsAg和抗-HBs水平的高(H)或低(L),将HBsAg+/抗-HBs+血清学模式分为H/L、H/H、L/L和L/H四型,分别占43.0%、14.0%、35.5%和7.5%;其HBeAg+比例依次为H/L(55.0%)>H/H(38.5%)>L/L(18.2%)>L/H(14.3%);H/L与L/L型患者HBV DNA水平之间差别显著(P=0.016),而在H/L与H/H(P=0.569)以及H/H与L/L之间(P=0.095)均无显著性差别.结论 在HBsAg和抗-HBs共存患者,HBsAg水平越低和抗-HBs水平越高,则HBeAg阳性率和HBV DNA水平越低.

  8. Prevention of de novo HBV infection by the presence of antiHBs in transplanted patients receiving core antibody-positive livers

    Institute of Scientific and Technical Information of China (English)

    Rafael Barcena; Gloria Moraleda; Javier Moreno; M Dolores Martín; Emilio de Vicente; Jesús Nu(n)o; M Luisa Mateos; Santos del Campo

    2006-01-01

    AIM: To analyze whether the presence of anti-HBs in liver transplant recipients is effective in preventing HBV infection.METHODS: Twenty-three patients receiving anti-HBc positive liver were studied. Nine recipients were anti HBc positive as a result of previous HBV infection. Of them, one also received HBV vaccine during the pre-liver transplantation period. Fourteen recipients were anti-HBs positive due to HBV vaccine administered during the pretransplant period. Liver biopsy was obtained in 10/14anti-HBc negative/anti-HBs positive recipients and in 4/9anti-HBc positive recipients.RESULTS: After a mean follow-up period of 46 months,1 recipient with protective serum anti-HBs levels developed de novo HBV infection as a consequence of immune escape HBV mutants. Among the 14 vaccinated anti-HBc negative/anti-HBs positive recipients, 1/10patients with available liver biopsy (10%) had liver HBVDNA at 13 mo post-liver transplantation without serum viral markers and did not develop de novo HBV infection.The vaccinated anti-HBc positive recipient without HBV vaccine response was HBV-DNA positive in serum and liver, viral DNA was continuously negative in the followlng tests, so a spontaneous seroconversion was diagnosed.CONCLUSION: The presence of anti-HBs as a result of HBV vaccine or past HBV infection seems to be effective at protecting patients receiving livers from anti-HBc positive donors. However, the emergence of immune escape HBV mutants, which can evade the anti-HBs protection, should be considered as a risk of HBV infection.

  9. The one year effects of three doses of hepatitis B vaccine as a booster in anti-HBs-negative children 11-15 years after primary immunization; China, 2009-2011.

    Science.gov (United States)

    Yao, Jun; Shan, Huan; Chen, Yongdi; Jiang, Zheng-gang; Dai, Xue-wei; Ren, Jing-jing; Xu, Kai-jin; Ruan, Bing; Yang, Shi-gui; Li, Qian

    2015-01-01

    The aim of this study was to evaluate hepatitis B surface antibody (anti-HBs) levels one year after hepatitis B booster vaccination in anti-HBs-negative (Anti-HBs titers were examined in 235 children who were negative for hepatitis B surface antigen (HBsAg), anti-HBs, and hepatitis B core antibody (anti-HBc). The children were then divided into 3 groups based on their anti-HBs levels pre-booster: Group I, anti-HBs levels were measured. One month after the first dose, the anti-HBs positive rates (≥ 10 mIU/mL) in Groups I-III were 56.14%, 83.61% and 100%. One month after the third dose, the anti-HBs-positive rates in Groups I-III were 96.49%, 98.36% and 100%. One year after the third dose, the anti-HBs-positive rates in Groups I-III were 73.68%, 75.41% and 98.29%, respectively. Protective levels declined more rapidly for those with lower titers. Children with pre-booster anti-HBs titers of 1-9.9 mIU/mL might not need any booster dose, and the children with pre-booster titers of 0.1-0.9 and <0 .1 mIU/mL might need more than one dose booster vaccination.

  10. Awareness and Practice of Complete Hepatitis B Vaccination and Anti-HBs Testing in Vaccinated Health Care Workers

    Directory of Open Access Journals (Sweden)

    Annapurna G. Sajjan

    2015-04-01

    Full Text Available Background: Hepatitis B is a serious and common infectious disease affecting millions of people worldwide. Health Care Workers (HCW are at an increased risk of occupational exposure to HBV and the incidence is 2-4 times higher than in the general population. Despite potential risks, awareness and vaccine compliance is poor among the HCWs. Aim: To assess the awareness of complete Hepatitis B vaccination, anti-HBs testing & protective titres and determine the anti HBs titres amongst vaccinated HCWs. Material & Methods: A total of 500 Health care workers of both sexes in the age group from 20- 60 years vaccinated against Hepatitis B were tested for anti-HBs titres by quantitative ELISA. Results: The rate of complete immunization was 81.4% in doctors, 63.3% in nursing staff and 90% in the technical staff. Amongst the 500 participants, 70.8% had received all the doses and 29.2% incomplete doses of the vaccine. Titres of ≥ 10 mIU/ml were demonstrated in 84.4% of HCWs who received all the doses and in 65.7% those who defaulted. Conclusions: The results of the study indicate lack of awareness about complete HB vaccination and the importance of post vaccination testing in HCWs.

  11. HBs Fab-IFNα融合蛋白的制备与初步鉴定%Preparation and preliminary identification of anti HBs Fab-IFNa fusion protein

    Institute of Scientific and Technical Information of China (English)

    陆慧琦; 宋杰; 叶伟民; 韩焕兴

    2011-01-01

    目的 构建抗HBsAg的pComb Fab-IFNα载体,原核表达具双重生物活性的抗HBs Fab-IFNα融合蛋白.方法 以pBAD-Fab和pBAD IFNα作为模板,分别扩增Fd、λ和IENα基因,经相应的限制性内切酶酶切后分3次克隆人pComBHss质粒,转化XL-1 Blue大肠埃希菌.限制性酶切和测序鉴定重组质粒,免疫蛋向印迹(Western blotting)和斑点印迹(Dot blotting)鉴定融合蛋白的表达及抗原结合活性.结果 重组载体的酶切、电泳及测序表明抗HBs Fab-IFNα基因克隆正确.表达产物经12%SDS-PAGE电泳、转印,Western blotting显示该融合蛋白分子量约为65kD,Dot blotting显示其与HBsAg具有结合能力.细胞病变抑制法测定IFNα生物学活性为7.8×104~5.1×105U/ml.结论 该原核系统成功表达了抗HBs Fab-IFNα融合蛋白,表明其既具有抗HBsAg结合能力,又具备IFNα的生物活性,为进一步的系统表达和应用研究提供了条件.%Objective To construct the anti HBsAg pComb Fab-IFNe vector, and to express the fusion protein consisting of IFN and anti-HBs Fab in prokaryon. Methods Using pBAD-IFN plasmid and pBAD-Fab plasmid as template, the anti-HBs Fd, and IFN were amplified separately with corresponding endonuclease sites by polymerase chain reaction (PCR). Each PCR product was digested with specific endonuclease and inserted into pComBHss vector, and then transformed into XL-1 Blue. The recombinant plasmid was isolated by miniprep for restriction analysis and sequencing. Fusion protein was identified by Western blotting and Dot blotting. Results The recombinant plasmid was confirmed by restriction electrophoresis and sequencing. Aliquots of human anti-HBs Fab-IFNα were concentrated and size fractionated by 12% SDS-PAGE and stained with Coomassie. The proteins were transferred to nitrocellulose, incubated with HRP- conjugated goat anti-human IgG Fab and Rah anti-human IFN. The molecular weight of the fusion protein was about 65kD. Anti HBs Fab-IFNa fusion

  12. 从159例HBsAg、Anti-HBs的测定谈乙型肝炎免疫预防的必要性

    Institute of Scientific and Technical Information of China (English)

    莫宁萍; 凌锌

    2001-01-01

    目的对159份血清进行HBsAg,Anti-HBs检测,并进一步分析乙型肝炎(以下简称乙肝)的预防.方法HBsAg,Anti-HBs的测定用ELISA法.结果HBsAg携带者为13人,无免疫力者为78人,Anti-HBs阳性68人.结论血清学测定HBsAg.Anti-HBs对于及早发现乙肝病毒携带者或易感者有准确的效果,对于乙肝的防治有较好的辅助作用.

  13. Low dose revaccination induces robust protective anti-HBs antibody response in the majority of healthy non-responder neonates.

    Science.gov (United States)

    Jafarzadeh, A; Zarei, S; Shokri, F

    2008-01-10

    A sizeable proportion (1-10%) of healthy adults and to lesser extent neonates vaccinated with triple 10 microg hepatitis B (HB) vaccine fail to mount a protective antibody response. Revaccination with the same vaccine dose has proved to be effective in a significant number of primary non-responders. The influence of revaccination with lower vaccine doses however has not been studied adequately in non-responder neonates. This study was conducted to evaluate the influence of supplementary vaccination with a single low and standard dose of a recombinant hepatitis B (HB) vaccine in healthy Iranian non-responder neonates to primary vaccination. Iranian neonates unable to respond to primary vaccination with 10, 5 or 2.5 microg doses of recombinant HB vaccine were revaccinated with a single additional dose of the same concentration. Serum anti-HBs antibody titer was measured by sandwich ELISA. Administration of a single additional dose induced seroprotection (anti-HBs> or =10IU/L) in 10/12 (83%), 10/12 (83%) and 21/24 (87.5%) of non-responder neonates in 10, 5 and 2.5 microg vaccine recipients with geometric mean titers (and 95% confidence limits) of 1358 (258-7142), 401 (79-2038) and 164 (62-433) IU/L, respectively. The log-transformed antibody titer obtained for the 10 microg dose recipients was significantly higher than that of the 2.5 microg dose vaccinees (p=0.028). No significant differences in anti-HBs titer were observed between other groups of vaccinees. However, the total seroprotection rates obtained after administration of four low doses of 2.5 and 5 microg were significantly higher than that obtained after administration of the classical three 10 microg doses (p=0.029 and p=0.006, respectively). The total seroprotection rates were similar between all groups of vaccines receiving four doses of 2.5, 5 and 10 microg vaccine doses. These results indicate that a significant proportion of non-responder neonates can be induced to develop a protective anti-HBs

  14. HLA-DPB1 and anti-HBs titer kinetics in hepatitis B booster recipients who completed primary hepatitis B vaccination during infancy.

    Science.gov (United States)

    Wu, T-W; Chu, C-C; Liao, H-W Chang; Lin, S-K; Ho, T-Y; Lin, M; Lin, H H; Wang, L-Y

    2014-01-01

    Previously we reported significant associations of the human leukocyte antigen (HLA)-DPB1 05:01 with memory against hepatitis B (HB) vaccination. However, the effects of HLA-DPB1 on antibodies to hepatitis B surface antigen (anti-HBs) kinetics were not explored. We followed up a cohort of 1974 HB booster recipients and quantified their 1-month and 1-year post-booster anti-HBs titers. A total of 681 subjects were randomly selected and typed for HLA-DPB1. We found that male subjects, undetectable pre-booster titers, and 05:01 homozygotes led to significantly lower post-booster anti-HBs titers. The geometric means (95% confidence interval (CI)) of 1-month post-booster anti-HBs titers were 4.68 (2.69-8.12), 23.01 (14.96-35.40) and 50.06 (27.20-92.13) mIU ml(-1) for subjects carrying two, one and no HLA-DPB1 05:01 allele. The corresponding figures for 1-year post-booster anti-HBs titers were 1.26 (0.73-2.18), 4.72 (3.08-7.25) and 7.32 (3.75-13.56) mIU ml(-1). There were significant associations of post-booster anti-HBs titers with the number of HLA-DPB1 risk and protective alleles. Among booster responders, anti-HBs decay rates were significantly reduced in subjects who had detectable pre-booster anti-HBs titers and the HLA-DPB1 05:01 allele. Our results indicated that HLA-DPB1 influences the kinetics of anti-HBs. The long-term memory against hepatitis B surface antigen (HBsAg) and the residual serum titers of anti-HBs after HB vaccination may be influenced by different mechanisms as evidenced by their inverse trend of associations with the 05:01 allele.

  15. Anti-HBs profiles in children treated for neoplastic disease who had been vaccinated against hepatitis B postnatally or as infants.

    Science.gov (United States)

    Koltan, S; Koltan, A; Wysocki, M; Debski, R; Styczynski, J

    2005-05-01

    Children with cancer are a risk group for hepatitis B virus (HBV) infection. In Poland, the initiation of a national HBV vaccination programme in neonates and infants in 1995 has contributed to the prevention of HBV infection in children treated for neoplastic diseases. The objective of this study was to analyse the anti-HBs pattern and occurrence of HBV infections in children with cancer who had been vaccinated during infancy. The study included 96 children divided into three groups: Group A, children who had received a full vaccination course with an initial level of anti-HBs >/=100 IU/L; Group B, children who had received a full vaccination course with anti-HBs anti-HBs after the full vaccination programme was found in 80.5% of children after three months, 74.2% after six months, 61.5% after 12 months and 78.6% after 18 months. Among children who produced antibodies, a slow decrease in the level of anti-HBs was observed. It was still protective during follow-up in Group A, but occasionally fell below 100 IU/L in Group B. In Group C, five of 11 subjects had a protective level of anti-HBs throughout follow-up. Of 28 children who showed the presence of HBsAg during follow-up, 23 eradicated the virus. In children with cancer vaccinated against HBV according to the vaccination schedule, the immune response maintains a protective level of anti-HBs in more than 60% of cases, despite immunosuppression.

  16. [Retrospective evaluation of HBsAg, anti-HCV, anti-HIV and syphilis reagin antibody seropositivity in blood donors at the Trabzon Farabi Hospital].

    Science.gov (United States)

    Aydin, Faruk; Cubukçu, Kivanç; Yetişkul, Serpil; Yazici, Yelda; Kaklikkaya, Neşe

    2002-01-01

    Transfusion of blood and blood products is a widely used method for therapy in medicine, however it may result with the transmission of infectious agents from donor to recipient. In order to achieve safe blood transfusions and to minimize post-transfusion infections, several screening tests for infectious agents are routinely done all around the world as well as in our country. In this study, HBsAg, anti-HCV, anti-HIV and syphilis reagin antibody tests results have been retrospectively evaluated for 33.766 blood donors during January 1, 1997 to December 31, 2000 in Blood Center of Farabi Hospital, Black Sea Technical University. Testing for HBsAg, anti-HIV and anti-HCV has been done by using commercially available micro and/or macro enzyme immunoassays, and syphilis reagin antibody test by latex agglutination (RPR) method. The indeterminate results were confirmed by retesting of sera with microparticle enzyme immunoassay and Western blot methods. As a result, in 1331 (3.94%) subjects HBsAg, in 250 (0.74%) subjects anti-HCV, and in 161 (0.47%) subjects RPR were found positive. Twenty samples which have had the results in gray-zone for anti-HIV, have been found negative with the confirmation tests.

  17. Analysis and clinial significance of the coexistence of HBsAg and anti-HBs%HBsAg和抗-HBs双阳性模式分析及临床意义

    Institute of Scientific and Technical Information of China (English)

    肖青; 朱金养; 谢理成

    2010-01-01

    目的 分析乙型肝炎病毒(HBV)血清标志物乙型肝炎表面抗原(HBsAg)和乙型肝炎表面抗体(抗-HBs)同时阳性的感染模式,探讨此类模式的产生原因及其临床意义.方法 应用时间分辨免疫荧光分析法定量检测10 939例血清标本,从中筛选出HBsAg和抗-HBs同时阳性的标本,进而分析其检测结果,并与酶联免疫吸附试验(ELISA)检测的12 000例血清标本得到的HBsAg和抗-HBs同时阳性的结果进行比较.结果 定量检测10 939例血清标本,其中HBsAg阳性3 338例,阳性率为30.5%,HBsAg和抗-HBs同时阳性者占0.8%(28/3 338);ELISA定性检测12 000例标本,HBsAg和抗-HBs同时阳性者占0.3%(12/12 000),统计学分析两种方法在检测HBsAg和抗-HBs同时阳性的结果,差异有统计学意义(P<0.05).该28例表现出4种类型HBV血清学指标的组合方式,以HBsAg、乙型肝炎e抗原(HBeAg)、乙型肝炎核心抗体(抗-HBc)阳性模式和HBsAg、乙型肝炎e抗体(抗-HBe)、抗-HBc阳性模式为主,分别有8例和17例;HBsAg、抗-HBs和抗-HBc阳性模式1例;其余2例仅HBsAg和抗-HBs双阳性.结论 HBsAg和抗-HBs同时阳性虽然少见,但具有一定的临床意义,应当引起实验室和临床的注意.

  18. Age-dependent decrease of anti-HBs titers and effect of booster doses using 2 different vaccines in Palestinian children vaccinated in early childhood.

    Science.gov (United States)

    Qawasmi, Mohammad; Samuh, Monjed; Glebe, Dieter; Gerlich, Wolfram H; Azzeh, Maysa

    2015-01-01

    Immunization against hepatitis B virus (HBV) has proven to be highly effective and led to significant reduction of new infections worldwide. However, protective immunity measured by anti-HBs titers may decrease to critical levels in the years after basal immunization, particularly in case of exposure to HBV variants different from the vaccine strain. We tested 400 Palestinian children between one and 19 years of age for their anti-HBs titer, challenged the immune memory of those with low or absent anti-HBs with 2 types of hepatitis B vaccines and determined thereafter the anti-HBs titer. At the age of one, 92.2% of the children presented with protective anti-HBs titers (≥ 10 mIU/ml) with the majority having ≥ 100 mIU/ml. Protective immunity was still high at ages 2 (87.5%) and 4 (95%), declining by age 5 and 6 (from 69.2% to 66.7%) and down to an average of 39.8% between the ages of 7 and 19. 160 children with a nonprotective or low immune response challenged with either the yeast-derived Engerix-B or the mammalian cell-derived preS1-containing Sci-B-Vac vaccine showed an anamnestic immune response. 92.4% and 85.9% of the children challenged with one dose Sci-B-Vac and Engerix-B presented with anti-HBs titers >100 mIU/ml respectively. Our results reveal that vaccine-induced protective anti-HBs titers against HBV decrease rapidly beyond the age of 6 in Palestinian children, but can be strongly enhanced with a single booster vaccine dose, independent of brand and antigen composition. Our data suggest that a booster vaccine dose against HBV during school years may be useful.

  19. Desarrollo de superficies modificadas oro - anticuerpo anti-PSPB para su uso como biosensor en la detección de preñez bovina

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    Justo Laíz

    2011-05-01

    Full Text Available En este trabajo se presentan los resultados preliminares para el desarrollo de un biosensor amperométrico destinado a la cuantificación de Proteína B Específica de la Preñez (PSPB en fluidos biológicos como medio de diagnóstico clínico precoz de la preñez bovina. A la superficie de oro modificada con ácido mercaptoundecanoico (MUA se fijaron anticuerpos anti-PSPB y peroxidasa de rábano (HRP. Se verificó la inmovilización de las biomoléculas mediante voltamperometría cíclica y medida del ángulo de contacto.Se estudió el comportamiento electroquímico del electrodo desarrollado por medio de voltamperometría cíclica y medidas a potencial constante, estableciendo la relación entre el cambio de corriente catódica (al potencial de reducción de la quinona y la concentración de PSPB. Esta relación se ve afectada por la formación de complejos antígeno – anticuerpo que limitan el acceso del sustrato al sitio activo de la enzima, provocando una disminución en la señal eléctrica.El electrodo modificado de oro - anti-PSPB – HRP respondió de forma inversamente proporcional a la concentración de PSPB a un potencial de -200 mV (contra electrodo de referencia Ag/AgCl, tanto por voltamperometría cíclica como a potencial constante, y por tanto podría ser la base para el desarrollo de un biosensor amperométrico para detectar precozmente la preñez bovina.

  20. A CCD-based reader combined quantum dots-labeled lateral flow strips for ultrasensitive quantitative detection of anti-HBs antibody.

    Science.gov (United States)

    Zhang, Xueqing; Li, Ding; Wang, Can; Zhi, Xiao; Zhang, Chunlei; Wang, Kan; Cui, Daxiang

    2012-06-01

    Herein we reported a CCD-based reader combined quantum dots-labeled lateral flow strips for ultrasensitive quantitative detection of anti-HBs antibody. The CdTe quantum dots were prepared, then were used to label Hepatitis B Virus surface antigen, and then were fabricated into lateral flow strips. The as-prepared lateral flow strips were used to test different concentration of anti-HBV surface antibodies. The CCD-based reader was designed and fabricated, the quantitative analysis software was compiled, and resultant CCD-based reader system was used for quantitative analysis of examined anti-HBs antibodies on the strips. Results showed that the quantum dots-labeled lateral flow strips could detect the anti-HBs antibody with the limitation concentration of 200 pg/mL, the CCD-based reader system could detect anti-HBs antibody with the sensitivity of 2 pg/mL. In conclusion, the prepared CCD-based reader combined quantum dots-labeled lateral flow strips can be used for quantitative detection of anti-HBs antibody in sera with the sensitivity of 2 pg/mL, and has great potential in applications such as ultrasensitive detection of HBV antigens or antibodies, and other tumor biomarkers in near future.

  1. Serological Analysis of the Hepatitis B Patients who had Positive HBsAg and Anti-HBs%HBsAg与抗-HBs同时阳性的乙型肝炎患者血清学分析

    Institute of Scientific and Technical Information of China (English)

    姜慧敏; 蔡云娥; 于爱娥

    2007-01-01

    乙型肝炎病毒(HBV)血清学标志物(HBV-M)模式主要体现为大三阳(HBsAg+、HBeAg+、抗-HBc+)和小三阳(HBsAg+、抗-HBe、抗-HBc+),但也存在许多不典型的模式。笔者主要分析了HBsAg与抗-HBs同时阳性的乙型肝炎患者HBV-M及HBV感染状态。

  2. Anti-HAV seropositivity in adult patients with HBsAg positive from various locations of Turkey

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    Mesut ORTATATLI

    2012-04-01

    Full Text Available Objective: This study aims to determine the rate of hepatitis A virus (HAV seropositivity in adult HBsAg (+ patients from various regions of Turkey.Method: 137 adult (≥20 age male patients admitted to Erzurum Mareşal Çakmak Military Hospital in 2009 who were previously diagnosed as HBsAg(+ were included. The subjects were not vaccinated for HAV. Serum samples were analyzed by EIA (enzyme immunassay using Abbott/AxSYM. Chi-square test was used for statistical analysis of serological data.Results: The Anti-HAV IgG (+ rates was 83.2% in the study populations (114/137, 61.5% (8/13 for those from Marmara region, 83.3% (13/16 for Mediterranean region, 84.6% (22/26 for Mid-Anatolian region, 66.7% (8/12 for Blacksea region, 87.5% (21/24 for East Anatolian region, 94.1% (32/34 for Southeast-Anatolian region. According to our study, no significant difference was found between seven geographical regions due to HAV seropozitivity rates (χ2= 9.511, p=0.147. The seven geographical regions were classified two main grups as East-Southeast Anatolia and other regions. The percentage of anti-HAV positivity rate was significantly higher in East-Southeast Anatolia grup (91.4%; 53/58 comparedto other regions grup (77.2%; 61/79 (χ2= 4.803;p=0.028.Conclusion: The prevalance of Hepatitis A variesin different countries and even in different regionsof a specific country. Age, low socioeconomic leveland worse living conditions have been reportedas the most important risk factors in studies withhealthy individuals. In this study where subjects withHBsAg(+ were evaluated for anti-HAV positivity, anincrease in the risk was found as OR =3.13 times larger(95% confidence interval, 1.09-9.01 when especiallyliving conditions in East-Southeast Anatolia wascompared with other regions. It has been postulatedthat all patients monitored for chronic HBV infectionshould be assessed for anti-HAV IgG and negativeindividuals need to be vaccinated due to highermortality and more severe

  3. Effects of HBsAg pulsed dendritic vaccination on anti-HBs production in immunosuppressed rats after liver transplantation%HBsAg-树突状细胞疫苗对肝移植后免疫抑制状态大鼠抗-HBs产生的影响

    Institute of Scientific and Technical Information of China (English)

    李钺; 陈先锋; 刘作金; 龚建平; 李生伟

    2010-01-01

    目的 观察乙型肝炎表面抗原(HBsAg)冲击的树突状细胞(DCs)疫苗对肝移植(LT)术后处于免疫抑制状态大鼠的抗一HBs产生的影响.方法 Lewis大鼠LT术后(BN大鼠为供体)给予大剂量他克莫司(FK506,2 mg/kg),建立LT术后免疫抑制状态的动物模型,并随机分为两组.术后14 d及28 d,经腹腔注入HBsAg-DCs疫苗者为HBsAg-DCs组(15只),连续观察12周,注入HBsAg疫苗200 μl者为HBsAg组(15只);未予免疫抑制的LT术后Lewis大鼠为对照组(5只).切取肝脏组织行光、电镜检查;RT-PCR检测脾脏白细胞介素(IL)-2、干扰素(IFN)γ mRNA表达水平;酶联免疫吸附法检测血清IL-2、IFN γ及抗-HBs水平.两组间比较采用t检验,多组间比较采用单因素方差分析.结果 大剂量FK506使HBsAg-DCs组及HBsAg组动物处于高度免疫抑制状态,肝脏无排斥反应、IL-2及IFN γ表达水平明显低于对照组,t值为21.35~48.74,P值均<0.05; HBsAg-DCs组在疫苗注射后1、2、3个月均能检测到高滴度的抗-HBs,分别为(255.24±34.16)IU/L、(145.11±12.37)IU/L、(185.43±17.18)IU/L,明显高于HBsAg组(抗-HBs几乎为0),t值分别为45.24、47.69、58.38,P值均<0.05.结论 在LT术后处于免疫抑制状态的大鼠体内,HBsAg-DCs疫苗仍能诱导出高血清滴度的抗-HBs.%Objective To explore the effects of HBsAg pulsed dendritic vaccination on anti-HBs production in immunosuppressed rats after liver transplantation (LT). Methods Brown-Norway liver al-lografts were transplantated into Lewis reipients. The transplantated Lewis rats were injected with EK506 (2 mg/kg) and randomly divided into two groups: rats in HBsAg-DCs group (n = 15) were intraperitoneally injected with HBsAg pulsed DCs at 14 d and 28 d after LT, and rats in the HBsAg group (n = 15) were injected with HBsAg (200 μl) once a week for 12 weeks. Rats without any immunosuppressive treatment after LT served as controls (n = 5). IL-2 and IFN γ mRNA expression in spleen were analyzed

  4. The Serum Anti-HBs Level Among Children Who Received Routine Hepatitis B Vaccination During Infancy in Mianyang City, China: A Cross-Sectional Study.

    Science.gov (United States)

    He, Fang; Ma, Yuan-ji; Zhou, Tao-you; Duan, Jin-chao; Wang, Jun-feng; Ji, Yu-lin; Li, Hong; Zhang, Ju-ying; Tang, Hong

    2016-01-01

    Hepatitis B virus (HBV) prevalence has declined remarkably in children due to nationwide universal vaccination program for HBV in China. However, the persistence of immune response against HBV infection and the optimal time point when a booster vaccination should be performed remain to be elucidated. To assess the persistence and level of antibody against hepatitis B surface antigen (anti-HBs) in a representative population of age 15 and younger who received routine hepatitis B vaccination in Mianyang City, China. A cross-sectional study was conducted in 2011. One thousand five hundred twenty-six children of age 15 and younger who received three doses of 5 μg hepatitis B vaccine series during infancy but did not receive a booster vaccination later were enrolled. Of the 1,526 children, the mean age was 8.2 ± 4.1 and 739 children were male. The median anti-HBs level was 23.0 mIU/mL, and the total percentage of anti-HBs levels ≥10 mIU/mL was 60.9%. With an increase of age, median anti-HBs level, percentage of anti-HBs levels ≥10 mIU/mL, and percentage of anti-HBs levels ≥100 mIU/mL declined remarkably in the early period and reached the lowest level at the age of 3 and then remained relatively stable. The median anti-HBs level, the percentage of anti-HBs levels ≥10 mIU/mL, and the percentage of anti-HBs levels ≥100 mIU/mL in 1- and 2-year-old children were much higher than that in children aged 3-15 (p < 0.05, respectively). Immunity against HBV infection gradually decreased in early ages of children of 15 and younger who received three doses of 5 μg hepatitis B vaccine series during infancy in China. Three dosages of 10 μg hepatitis B vaccine for infants and repeated vaccination or additional booster vaccination for some children at or before age 3 should be provided to get much more powerful immunity to HBV.

  5. Fatal hepatitis B reactivation treated with entecavir in an isolated anti-HBs positive lymphoma patient: a case report and literature review.

    Science.gov (United States)

    Ferreira, Rosa; Carvalheiro, Joana; Torres, Joana; Fernandes, Alexandra; Giestas, Sílvia; Mendes, Sofia; Agostinho, Cláudia; Campos, Mário J

    2012-01-01

    Hepatitis B virus (HBV) reactivation is a well-recognized complication that occurs in lymphoma patients who undergo chemotherapy. Only very few cases of HBV reactivation in patients with isolated antibody against hepatitis B surface antigen (anti-HBs) have been reported. We present a case of a 78-year-old woman diagnosed with diffuse large B cell non-Hodgkin's lymphoma who only displayed a positive anti-HBs, as the single possible marker of occult HBV infection, before starting therapy. She was treated with several chemotherapeutic regimens (including rituximab) for disease relapses during 3 years. Forty days after the last cycle of chemotherapy, she presented with jaundice, markedly elevated serum aminotransferase levels, and coagulopathy. HBV serology showed positivity for HBsAg, anti-HBc and anti-HBs. HBV DNA was positive. Antiviral treatment with entecavir was promptly initiated, but the patient died from liver failure. A review of the literature of HBV reactivation in patients with detectable anti-HBs levels is discussed.

  6. Soroprevalência e fatores de risco para a infecção pelo vírus da hepatite B pelos marcadores AgHBs e anti-HBs em prisioneiros e primodoadores de sangue Seroprevalence and risk factors for hepatitis B by AgHBs and anti-HBs markers in prisoners and first-time donors, in Central Brazil

    Directory of Open Access Journals (Sweden)

    Celina Maria Turchi Martelli

    1990-08-01

    Full Text Available Foram rastreados 1.033 primodoadores de sangue e 201 prisioneiros, para a infecção pelo vírus da hepatite B (VHB, durante o período de junho de 1988 a fevereiro de 1989, em Goiânia, GO (Brasil. Foi estimada a soroprevalência dos marcadores AgHBs e anti-HBs, e estudados os fatores de risco associados a soropositividade. Os testes sorológicos foram realizados pela técnica de ELISA e a presença de qualquer dos marcadores estudados foi interpretada como exposição ao vírus da hepatite B. Um questionário padronizado foi aplicado em ambos os grupos populacionais para avaliar: transfusão sangüínea prévia, número de parceiros sexuais, atividade homo/bissexual, história de outras doenças sexualmente transmissíveis, uso de droga injetável, uso de medicação injetável, acupuntura, tatuagem e soropositividade ao VDRL. Foram obtidas soroprevalências globais para a infecção pelo VHB de 26,4% e 12,8% para a população carcerária e de primodoadores respectivamente, diferença estatisticamente significante (pTwo cross-sectional surveys on hepatitis B virus (HBV infection were carried out among 1,033 volunteer first-time blood donors in five blood banks (3 private, 2 public and among 201 prisoners in the Penitentiary Center of Industrial Activity, in Goiânia, Central Brazil, between June 1988 and February 1989. Those surveys were part of a major study designed to estimate seroprevalence of HBsAg and anti-HBsAg markers by ELISA test, and to study risk factors associated with seropositivity. The presence of any serum marker was considered as previous exposure to HBV. A standard questionnaire was applied to both populations to evaluate previous blood transfusion, number of sexual partners, homo/bisexual activity, history of sexually transmitted diseases, drug abusers, use of parenteral medicine, accupunture, tattooing and VDRL seropositivity. Seroprevalence varied from 12.8% to 26.4% in blood donors and prisoners, respectively, (p<0

  7. Determinación de anticuerpos anti-β2glicoproteína I en pacientes con síndrome antifosfolípido Anti- β2 glycoprotein antibodies in patients with antiphospholipid syndrome

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    Oscar Uribe Uribe

    2004-09-01

    Full Text Available EL objetivo de este estudio fue comparar la presencia de anticuerpos anti-β2glicoproteína I (anti- β2GPI con las pruebas convencionales de laboratorio de anticuerpos anticardiolipina (aCL y anticoagulante lúpico, y con las manifestaciones clínicas del síndrome antifosfolípido (SAF. Se incluyeron en el estudio 80 mujeres con SAF; 35 de ellas de la consulta de Reumatología y las otras 45 con historia de aborto recurrente espontáneo (ARE; 5 mujeres de la consulta de Reumatología sin SAF, 27 mujeres con ARE, sin SAF y un grupo control de 20 mujeres sanas en edad reproductiva. Se investigaron la presencia de anticuerpos IgG e IgM anticardiolipina (aCL e IgG anti- β2GPI por la técnica de ELISA, y el anticoagulante lúpico por la determinación del tiempo parcial de tromboplastina activado. Adicionalmente, se registraron las manifestaciones clínicas asociadas al SAF. De las pacientes con SAF, 25.7% del grupo de Reumatología (9/35 y 4.4% de las pacientes con ARE (2/45 fueron positivas para anticuerpos anti-β2GPI, mientras que ninguna de las mujeres sin SAF, ni de las mujeres del grupo control, fue positiva. La asociación entre la presencia de anti- β2GPI y los anticuerpos IgG e IgM aCL mostró una diferencia significativa en los títulos de 3+ (altamente positivos en contraste con los individuos negativos para anti- β2GPI. La positividad del anticoagulante lúpico también se correlacionó con la presencia de anticuerpos anti- β2GPI. No hubo diferencia significativa entre las diversas manifestaciones clínicas del SAF y la presencia de dichos anticuerpos. En conclusión, la determinación de anticuerpos anti- β2GPI tiene una alta especificidad en pacientes con SAF pero no se asoció con ninguna manifestación clínica en particular. The objective of this study was to compare the presence of anti-β2glycoprotein (anti- β2GPI antibodies with the conventional laboratory tests of anticardiolipin antibodies (aCL and lupus anticoagulant

  8. Intestinal helminthes and/or Toxocara infection are unrelated to anti-HBs titers in seven-year-old children vaccinated at birth with recombinant hepatitis B vaccine.

    Science.gov (United States)

    Monteiro, Marisa B C L; Fragoso, Roberta; Foletto, Silvio; Lemos, Elenice M; Pereira, Fausto E L

    2007-01-01

    The aim of this investigation was to evaluate the possible effect of nematode infection on anti-HBs antibody levels in the serum of seven-year-old schoolchildren vaccinated at birth with the recombinant hepatitis B vaccine. Anti-HBs and anti HBc antibodies were evaluated in the sera of 100 schoolchildren with at least one intestinal nematode and/or a positive serological reaction for anti-Toxocara antibodies and in 95 schoolchildren without intestinal helminthiasis or serum anti-Toxocara antibodies. Both groups were from public elementary schools located on the urban periphery of Vitória, ES, Brazil. Among these 195 children, the median anti-HBs antibody titer was 31.3 IU/ml and the frequency of titers less than 10 IU/ml was 33.8% (95% CI: 27.1-40.4%). There were no significant differences between the medians of anti-HBs titers or the frequency of titers less than 10 IU/ml between the groups with or without helminthes (29.5 and 32.9 IU/ml and 33 and 34.7%, respectively; p>0.05). Even when the children with intestinal nematodes and/or anti-Toxocara antibodies and with blood eosinophil counts over 600/mm(3) were compared with children without infection from intestinal nematodes and without anti-Toxocara antibodies, with blood eosinophil counts less than 400 eosinophils/mm(3), these differences were not significant. None of the children presented anti-HBc antibodies. In conclusion, infections with intestinal nematodes and/or the presence of anti-Toxocara antibodies did not interfere with the anti-HBs antibody titers in seven-year-old children vaccinated at birth with the recombinant hepatitis B vaccine.

  9. Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix Association between anti-Ras and anti-HPV16 E4/E7 antibodies with cervical intraepithelial lesions

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    Sara Vázquez-Corzo

    2003-10-01

    Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.OBJECTIVE: To evaluate whether serum antibodies anti-E4, E7 and Ras could be used as markers for early cervical lesions associated with HPV (human papillomavirus. MATERIAL AND METHODS: A seroepidemiological case-control study was conducted between March 1999 and April 2000 at the dysplasia clinic of Hospital General Doctor Gea Gonzalez, in Mexico City, to evaluate the presence of antibodies anti-E4, E7, and Ras through a sandwich ELISA. Analysis was done using odds ratios and 95% confidence intervals. RESULTS: Anti-E7 antibodies were associated to women with CIN III lesions, while anti-E4 and Ras antibodies were strongly associated with CIN I-II lesions. The antibody profile of women with different

  10. The persistence of anti-HBs antibody and anamnestic response 20 years after primary vaccination with recombinant hepatitis B vaccine at infancy.

    Science.gov (United States)

    Bagheri-Jamebozorgi, Masoomeh; Keshavarz, Jila; Nemati, Maryam; Mohammadi-Hossainabad, Saeed; Rezayati, Mohammad-Taghi; Nejad-Ghaderi, Mohsen; Jamalizadeh, Ahmad; Shokri, Fazel; Jafarzadeh, Abdollah

    2014-01-01

    Hepatitis B (HB) vaccine induces protective levels of antibody response (anti-HBs≥10 mIU/mL) in 90-99% of vaccinees. The levels of anti-HBs antibody decline after vaccination. The aim of this study was to evaluate the persistence of anti-HBs antibodies and immunologic memory in healthy adults at 20 years after primary vaccination with recombinant HB vaccine. Blood samples were collected from 300 adults at 20 years after primary HB vaccination and their sera were tested for anti-HBs antibody by ELISA technique. A single booster dose of HB vaccine was administered to a total of 138 subjects, whose anti-HBs antibody titer was anti-HBs antibody levels at 4 weeks after booster vaccination. At 20 years after primary vaccination 37.0% of participants had protective levels of antibody with geometric mean titer (GMT) of 55.44±77.01 mIU/mL. After booster vaccination, 97.1% of vaccinees developed protective levels of antibody and the GMT rose from 2.35±6.49 mIU/mL to 176.28±161.78 mIU/mL. 125/138 (90.6%) of re-vaccinated subjects also showed an anamnestic response to booster vaccination. At 20 years after primary vaccination with HB vaccine, low proportion of the subjects had protective levels of antibody. However, the majority of the re-vaccinated subjects developed protective levels of anti-HBs and showed an anamnestic response after booster vaccination. Additional follow-up studies are necessary to determine the duration of immunological memory.

  11. Long-term anti-HBs antibody persistence following infant vaccination against hepatitis B and evaluation of anamnestic response: a 20-year follow-up study in Thailand.

    Science.gov (United States)

    Poovorawan, Yong; Chongsrisawat, Voranush; Theamboonlers, Apiradee; Crasta, Priya Diana; Messier, Marc; Hardt, Karin

    2013-08-01

    Hepatitis B vaccine has been available worldwide since the mid-1980s. This vaccine was evaluated in a clinical trial in Thailand, conducted on subjects born to hepatitis B surface antigen positive and hepatitis B e-antigen positive mothers and vaccinated according to a 4-dose schedule at 0, 1, 2 and 12 mo of age and a single dose of hepatitis B immunoglobulin concomitantly at birth. All enrolled subjects seroconverted and were followed for 20 y to assess the persistence of antibody to the hepatitis B surface antigen (anti-HBs) (NCT00240539). At year 20, 64% of subjects had anti-HBs antibody concentrations≥10 milli-international units per milli liter (mIU/ml) and 92% of subjects had detectable levels (≥3.3 mIU/ml) of anti-HBs antibodies. At year 20, subjects with anti-HBs antibody titeranti-HBs antibody geometric mean concentrations. This study confirms the long-term immunogenicity of the 4-dose regimen of the HBV vaccine eliciting long-term persistence of antibodies and immune memory against hepatitis B for up to at least 20 y after vaccination.

  12. Analysis of Test Results of HBsAg anti-HBs among Shizuishan Students%石嘴山市学生HBsAg抗-HBs检测结果分析

    Institute of Scientific and Technical Information of China (English)

    李月英; 郭志明

    2010-01-01

    目的 了解三年来中小学生中HBsAg携带率和保护性抗体抗-HBs的产生率的变化情况.方法采用酶联免疫吸附法(ELLSA)对本市中小学生进行HBsAg、体抗-HBs检测,并进行结果分析.结果 我市中小学学生HBsAg总阳性率为1.74%,高中组高于初中组,初中高于小学组;抗-HBs阳性率小学组高于初中组,初中组高于高中组,且同年龄组中,随着年份的增加而增加.每年的HBsAg阳性率城市明显低于农村,每年的抗-HBs阳性率城市明显高于农村.结论 我市学生HBsAg总阳性率为1.74%,低于全国平均水平9.09%,属于低感染地区;保护性抗体抗-HBs的阳性率逐年增加,城市高于农村.

  13. Asociación del anticuerpo anti-C1q con las manifestaciones clínicas, hematológicas e inmunológicas en el Lupus Eritematoso Sistémico : Estudio observacional retrospectivo de 135 casos.

    OpenAIRE

    Córica, María Emilia

    2012-01-01

    : Las concentraciones de anticuerpo anti-C1q están elevadas en los pacientes afectados de LES. Según algunos estudios la presencia de este anticuerpo está asociada a la nefropatía lúpica, sin embargo en otros estudios esta relación no se comprobó. En nuestro trabajo relacionamos la positividad del anticuerpo anti-C1q con las manifestaciones clínicas, hematológicas e inmunológicas de la enfermedad. A diferencia de estudios previos sólo pudimos establecer una relación estadísticamente significa...

  14. Hepatitis B virus screening in contacts of blood donors with antibodies against core protein (anti-HBc, but without surface antigen (HBsAg

    Directory of Open Access Journals (Sweden)

    Hildenete Monteiro Fortes

    2006-03-01

    Full Text Available To increase blood safety Brazil introduced screening for anti-HBc among blood donors in 1993. There was a decrease in the hepatitis B virus (HBV transmission, but this measure identified a great number of HBsAg-negative, anti-HBc-positive donors. Surveillance policy determines that contacts of HBV carriers should be screened to HBV markers, but there is no recommendation about how to guide contacts of HBsAg-negative, anti-HBc-positive donors. Aiming to evaluate whether the contacts of this group are at greater risk for HBV infection, a cross-sectional study was performed to compare prevalence of HBV infection between contacts of HBsAg-positive blood donors (group I and contacts of HBsAg-negative, anti-HBc-positive donors (group II. Contacts were submitted to a questionnaire and blood tests for HBV markers. In group I (n = 143, 53 (37.1% were anti-HBc-positive and 11 (7.7% were HBsAg-positive. In group II (n = 111, there were 9 and 0.9%, respectively. HBV exposure was associated with group I, sexual activity, blood transfusion, being one of the donor's parents, and living for more than ten years with the donor. Regarding the families as sample units, it was more common to find at least one member with HBV markers (p < 0.05 among the families of group I compared to group II. Contacts of HBsAg-negative, anti-HBc-positive individuals presented a much lower risk of having already been exposed to HBV and there is no need to screen them for HBV in low to moderate prevalence populations.

  15. De Novo Superinfection of Hepatitis B Virus in an Anti-HBs Positive Patient with Recurrent Hepatitis C Following Liver Transplantation.

    Science.gov (United States)

    Ha, Sung-Hae; Park, Young-Min; Hong, Sun-Pyo; Back, So-Ya; Shin, Soo-Kyeong; Ji, Seung-Il; Kim, Soo-Ok; Yoo, Wang-Don; Kim, Bo-Hyun; Park, Sang-Jong; Hong, Zheng

    2011-06-01

    A 60-year-old woman with end stage liver cirrhosis caused by genotype 2 hepatitis C virus (HCV) infection received an orthotopic liver transplantation (OLT). The patient was negative for the hepatitis B surface antigen (HBsAg) and positive for the anti-hepatitis B surface antibody (anti-HBs) prior to and one and a half months following the OLT. Due to reactivation of hepatitis C, treatment with interferon-alpha and Ribavirin started two months following the OLT and resulted in a sustained virological response. We performed a liver biopsy because a biochemical response was not achieved. Surprisingly, liver pathology showed HBsAg-positive hepatocytes with a lobular hepatitis feature, which had been negative in the liver biopsy specimen obtained one and a half months post-OLT. High titers of both HBsAg and HBeAg were detected, while anti-HBs antibodies were not found. Tests for IgM anti-hepatitis B core antibody and anti-delta virus antibodies were negative. The serum HBV DNA titer was over 1×10(7) copies/mL. A sequencing analysis showed no mutation in the "a" determinant region, but revealed a mixture of wild and mutant strains at an overlapping region of the S and P genes (S codon 213 (Leu/Ile); P codons 221 (Phe/Tyr) and 222 (Ala/Thr)). These findings suggest that de novo hepatitis B can develop in patients with HCV infection during the post-OLT period despite the presence of protective anti-HBs.

  16. Anti-preS responses influence the anti-HBs response in newborns after vaccination with the third generation Sci-B-Vac vaccine.

    Science.gov (United States)

    Sylvan, Staffan P E; Madalinski, Kazimierz; Hellström, Ulla B

    2009-12-11

    We analysed the specificity and significance of the antibody response towards the linear preS1 sequence that has been shown to represent the "hepatocyte binding site" comprising amino acids preS1 (21-47) or the specific preS2 (131-140) antibody response to the "polymerised albumin receptor" in relation to the antibody response to hepatitis B surface antigen during immunisation of healthy children with the preS-containing Sci-B-Vac vaccine. Twenty-eight healthy newborns received three doses of the Sci-B-Vac vaccine according to a 0-, 1-, and 6-month scheme. Seventeen (61%) of the 28 newborns had detectable levels of anti-preS1 (21-47) antibodies and 14 (50%) were anti-preS2 (131-140) reactive at 6 and/or 9 months after initiation of the vaccination. The mean levels of anti-HBs were significantly higher in the anti-preS2 (131-140) non-reactive (24580+/-7815IU/l, mean+SEM) compared with the reactive sera (7287+/-2317IU/l, panti-HBs levels were found in newborns who exhibited reactivity towards the aa 21-47 of the preS1 but lacked anti-preS2 (131-140) reactivity.

  17. 孕期注射乙肝免疫球蛋白对婴儿anti-HBs产生的影响%The effect ofhepatitis B immunoglobulin administration during pregnancy in the produce anti- HBs in infants

    Institute of Scientific and Technical Information of China (English)

    刘洁; 李淑红; 刘志芬; 刘绍菲; 贾蓓; 董梅

    2011-01-01

    Objective; To study the efficacy of hepatitis B immunoglobulin (HBIG) administrated during pregnancy for the infants immune response to hepatitis B vaccine through a follow - up study program. Methods: 300 HBsAg positive pregnant women who were consecutively collected and were administered with HBIG were selected as intervention group, 80 HBsAg positive pregnant women who were not administered with HBIG were selected as controls. The titer of anti - HBsAg at birth and 7th months were detected. Results; The rate of detectable anti - HBs in infants waslO. 3% (31/300) and 1. 25% ( 1/80) among in intervention group and control group. After infants were inmmunized by HBIG combining hepatitis B vaccine, the anti - HBs positive rate reached 96. 1% (124/ 129) and 95. 3% (41/43) at 7th months. Of the 17 infants HBsAg positive at birth, HBsAg was found persistently sera positive in 1 neonate at two years old, the liver function is normal, the others all became HBsAg negative at 7th month, among the 147 infants anti - HBs negative at birth, there were 7 infants who had no detective anti - HBs at7th month, however, of the25infants having detective anti - HBs at birth, all infants detected anti - HBs at 7th month. Conclusions: The results of the present study suggested that HBIG administration during pregnancy can raise the rate of anti - HBs at birth but but there is no significance at 7th month.%目的 探讨孕期注射乙肝免疫球蛋白的孕妇分娩的婴儿对乙肝疫苗免疫应答情况.方法 以HBsAg阳性孕妇及其新生儿为研究对象,孕期母亲注射乙肝免疫球蛋白的300例新生儿为实验组,未使用者80例为对照组,比较两组新生儿出生时及联合免疫后7个月anti - HBs产生情况.结果 实验组与对照组婴儿出生时anti - HBs阳性率分别为10.3%(31/300)和1.25% (1/80),差异有统计学意义;7个月龄实验组anti - HBs产生率为96.1% (124/129),对照组anti -HBs产生率95.3% (41/43),差异无统计学意义;出生时anti

  18. Patrón de immunoblotting y niveles de anticuerpos anti-Toxoplasma gondii en suero y humor acuoso de pacientes con lesiones de toxoplasmosis ocular

    Directory of Open Access Journals (Sweden)

    Morella Bouchard Pereira

    2014-07-01

    Full Text Available El objetivo de este estudio fue evaluar en muestras de suero y humor acuoso los niveles de anticuerpos anti-toxoplasma a través del Coeficiente de Goldmann y Witmer (CGW y el patrón de reconocimiento antigénico a través del immunoblotting (IB, en pacientes con serología positiva con y sin lesiones de toxoplasmosis ocular. Se recogieron simultáneamente muestras de suero y humor acuoso de 26 pacientes: un grupo de casos que poseían lesiones retinales de toxoplasmosis ocular en fase activa e inactiva (n=17 y un grupo control que requería cirugía ocular por presencia de cataratas (n=9. Las determinación de IgM e IgG específicas se realizó por ELISA de inmunocaptura e indirecto, respectivamente. Se utilizó la inmunodifusión radial para la cuantificación de la IgG total. El CGW resultó >2, indicativo de producción local de anticuerpos específicos en 12/17 de los casos, mientras que en los controles no se observó, esto evidenció una sensibilidad del 71% y una especificidad de 100%. En IB, la aparición de bandas diferentes en humor acuoso, indicativo de producción local de anticuerpos específicos se observaron en 11/17 de los casos y 1/9 de los controles, reflejando una sensibilidad de 65% y una especificidad de 89%. Al considerar las dos pruebas la sensibilidad se incrementó a un 73%, pero la especificidad disminuyó a 89%. En conclusión el IB es útil como prueba confirmatoria para diagnóstico de toxoplasmosis ocular, pero sólo como un complemento del coeficiente de GW especialmente en pacientes con lesiones atípicas donde el diagnóstico clínico es difícil. Aqueous humor and serum immunoblotting profiles and anti–toxoplasma gondii antibodies in patients with toxoplasmosis-induced retinal lesions Abstract The purpose of this study was to analize the anti-Toxoplasma gondii antibodies levels in serum and aqueous humor samples in patients with ocular toxoplasmosis by using Goldman and Witmer Coefficient (GWC and the

  19. Detección de anticuerpos Anti-brucella en focas de Weddell (Leptonychotes weddellii de Cabo Shirref, Antártica Detection of anti-brucella antibodies in Weddell seals (Leptonychotes weddellii from cape Shirref, Antarctica

    Directory of Open Access Journals (Sweden)

    O. BLANK

    2002-01-01

    Full Text Available Posterior al hallazgo de anticuerpos anti-Brucella en muestras de lobo fino antártico (Arctocephalus gazella, el estudio serológico en Pinnipedia Antárticos se continuó con el fin de determinar la presencia de anticuerpos anti-Brucella en otras especies. Se colectaron muestras de sangre y de fluido extravascular de 12 ejemplares de foca de Weddell (Leptonychotes weddellii encontrados en el Sitio de Especial Interés Científico (SEIC Nº 32 y sitio CCAMLR Ecosystem Monitoring Program (CEMP N° 2, "Cabo Shirreff e Islotes San Telmo" (62º 47' S; 60º 27' W, localizado en la costa Noroeste de la isla Livingston (Shetland del Sur, Antártica. Las muestras obtenidas fueron analizadas por la técnica convencional Rosa de Bengala (RB y dos inmunoensayos enzimáticos de competencia: Compelisa®, y c-ELISA. De las muestras estudiadas se identificaron cinco con anticuerpos anti-Brucella, siendo las pruebas inmunoenzimáticas las técnicas más sensibles. Estos resultados muestran una alta probabilidad de presencia de infección por una bacteria del género Brucella en ejemplares L. weddellii y plantean la necesidad de realizar estudios complementarios, que permitan conocer la etiología y entender la epidemiología de Brucella en esta región del mundoAfter the finding of anti-Brucella antibodies in samples of Antarctic fur seal (Arctocephalus gazella, the serological study on Antarctic Pinniped was continued in order to determine the presence of anti-Brucella antibodies in other species. Blood and extra vascular fluid samples were taken from 12 Weddell seals (Leptonychotes weddellii at the Site of Special Scientific Interest (SSSI Nº 32 and CCAMLR* Ecosystem Monitoring Program (CEMP site N° 2 "Cape Shirreff and San Telmo Islets" (62º 47' S; 60º 27' W, located on the Norwest coast Livingston Island (South Shetland Islands, Antarctica. The samples were tested by the conventional Rose Bengal test (RB and two competitive enzymatic immunoassay

  20. HBsAg、抗-HCV反应性无偿献血者归队检测模式探讨%Study on rejoin detection mode for unpaid blood donors with HBsAgor anti-HCV positive reactivity

    Institute of Scientific and Technical Information of China (English)

    曾劲峰; 陈云龙; 郑欣; 刘衡; 邬林枫; 杜丹丹

    2014-01-01

    Objective To investigate the detection mode for those unpaid blood donors screening as positive HBsAg+ and/or positive anti-HCV could be permitted to recall again for re-detection under the regulation condition in order to determine whether or not regain their qualifications of donating blood and rejoin the blood donation again.Methods The unpaid blood donors preliminari-ly screening as positive HBsAg and/or positive anti-HCV in Shenzhen from Otcober 2007 to December 2013 and conforming to the rules for recalling to re-detection formulated by our center were analyzed and researched for conducting the feasibility discussion on the rejoin mode of unpaid blood donors.Results A total of 415 759 case-times of blood donation were conducted during 2007 ~2013.Among them,2 506 cases(0.60%)and 1 357 cases(0.33%)were screened as positive HBsAg or positive anti-HCV,respec-tively.The recall process of rejoin re-detection was initiated in 59 positive HBsAg donors and 16 positive anti-HCV donors with many times of blood donation.But only 31 positive HBsAg donors and 9 positive anti-HCV donors successfully completed the detec-tion items of re-detection process.Among them 29 positive HBsAg donor regained the qualifications of donating blood and 2 cases were shielded for the blood donation qualification due to unqualification in the following detection.All of the 9 recalled donators with positive anti-HCV regained the blood donation qualification.Conclusion Under present detection mode,the detection tech-nique of blood screening is hard to avoid the occurrence of false positive results caused by the reagents,instruments and personnel operating.In order to protect the donation qualifications of the unpaid blood donators,a set of scientific,reasonable and practical re-detection mode for rejoin of the blood donators should be established for protecting the limited blood donation resource.%目的:探讨深圳地区无偿献血人群因血液筛查检测 HBsAg 或抗-HCV 呈阳性反

  1. Neuromielitis óptica con alta expresión de acuaporina-4 y anticuerpos anti-acuaporina-4 positivos en suero

    Directory of Open Access Journals (Sweden)

    Alejandra Báez

    2012-04-01

    Full Text Available La presencia de anticuerpos IgG en suero, con blanco en los canales de acuaporina-4, es específica de la neuromielitis óptica (NMO. El 60% de los pacientes con NMO presentan lesiones cerebrales en la resonancia magnética (RM; en un 8% (mayoría niños estas lesiones se consideraron "atípicas". Presentamos dos pacientes con NMO y lesiones en el SNC de alta expresión de acuaporina-4. Caso 1: varón de 50 años, que comenzó con pérdida de visión en ojo derecho (OD. Recibió tratamiento empírico con metilprednisolona 1 g/d x 3 días. Al mes presentó dolor generalizado y hemiparesia derecha; nuevamente recibió metilprednisolona 1 g/d x 5 días e IgG IV 400 mg/kg/d × 5 días. Recuperó la deambulación persistiendo el dolor y fenómenos paroxísticos en los 4 miembros. Potenciales evocados visuales: P100, ojo izquierdo (OI 123 mseg. OD sin respuesta. La RM de cerebro (FLAIR mostró hiperintensidad en nervio óptico derecho, hipotálamo y comisura blanca anterior. RM cervical: lesión medular extensa (5 cuerpos vertebrales. Caso 2: mujer de 53 años, con disminución de la agudeza visual en ambos ojos y parestesias en miembros inferiores que remitieron espontáneamente. Evolucionó al mes con cuadriparesia e incontinencia esfinteriana. Recibió metilprednisolona 1 g/d x 5 días, sin mejoría. Potenciales evocados visuales: P100 OI 124 mseg. OD 128 mseg. RM cerebro: (FLAIR hiperintensidad hipotalámica y periacueductal. RM cervical: lesión medular extensa (7 cuerpos vertebrales. Anticuerpos anti-acuaporina-4 positivos en ambos pacientes (inmunofluorescencia indirecta. Las lesiones consideradas "atípicas", como aquí, en sitios con alta densidad de proteínas canales de agua AQP4 deberán considerarse para el diagnóstico diferencial.

  2. Purification and Analysis of Structure of Fab Fragment of Recombinant Human Anti-HBs%重组人抗HBs-Fab抗体的纯化及结构分析

    Institute of Scientific and Technical Information of China (English)

    饶桂荣; 陈文吟; 粟宽源; 任向荣; 郑业华; 余宙耀

    2006-01-01

    目的研究重组人抗HBs-Fab抗体的纯化工艺,并对其结构等特性进行分析.方法采用离子交换-分子筛层析法分离纯化由酵母工程菌(GS115/Fab)发酵的重组人抗HBsAg Fab抗体,并经ELISA检测其抗体活性,等电聚焦电泳法检测其等电点(pI),基质辅助激光解吸飞行时间质谱(MALDI-TOF-MS)检测其相对分子质量和肽质量图谱.结果纯化的重组Fab抗体的纯度可达90%以上,经Sephacryl-100进一步层析后,纯度达99%以上,总收率可达80%以上.Fab抗体具有较好的抗体活性,其pI值为7.6,为一碱性蛋白,相对分子质量为50 494,比其理论值约多2 579.35,经Endoglycosidase H内切酶消化后的相对分子质量为49 609,证明Fab的一级结构中有糖基化现象,且分布于Fab抗体的H链和L链上.胰酶酶解肽段中有21个与理论肽段相符,另检测到1对二硫键正确.结论已建立了重组人抗HBs-Fab抗体的稳定的纯化工艺,且Fab抗体的一级结构正确.

  3. Effect of revaccination using different schemes among adults with low or undetectable anti-HBs titers after hepatitis B virus vaccination.

    Science.gov (United States)

    Lin, Chao-Shuang; Xie, Shi-Bin; Liu, Jing; Zhao, Zhi-Xin; Chong, Yu-Tian; Gao, Zhi-Liang

    2010-10-01

    Our objective was to investigate the effect of various reimmunization schemes for hepatitis B in adults with low or undetectable anti-HBs titers. Over 2 years, 10 μg of Saccharomyces cerevisiae-recombinant hepatitis B virus (HBV) vaccine (synthesized in China) was used in at least one standardized scheme to immunize 2,310 healthy male and nonpregnant female adults. Of these, 240 subjects tested negative for hepatitis B markers. These 240 subjects were equally divided into 4 groups. The first group, designated Engerix-40, was revaccinated with 40 μg Engerix-B; the second, Engerix-20, was revaccinated with 20 μg Engerix-B; the third, Chinese-20, was revaccinated with 20 μg Chinese-made yeast-recombinant vaccine; and the last group, Chinese-10, was revaccinated with 10 μg Chinese-made yeast-recombinant vaccine. Blood samples were collected before and 1, 2, 8, and 12 months after the first injection. The anti-HBs-positive conversion rates of the Engerix-40, Engerix-20, and Chinese-20 groups were higher than that of the Chinese-10 group (P anti-HBs conversion rate increased in all groups, but values were significantly different from those for the other groups only in the Chinese-10 group (P anti-HBs geometric mean titers (GMTs) of the Engerix-40, Engerix-20, and Chinese-20 groups were higher than in the Chinese-10 group (P anti-HBs titers in subjects with low or undetectable titers after HBV vaccination.

  4. Influence of single nucleotide polymorphisms of cytokine genes on anti-HBs antibody production after hepatitis B vaccination in a Japanese young adult population.

    Science.gov (United States)

    Yukimasa, Nobuyasu; Sato, Shoichi; Oboshi, Wataru; Watanabe, Toru; Uzawa, Ryuichi

    2016-01-01

    Hepatitis B (HB) vaccination is one of the most efficient tools to prevent the transmission of the virus. Considerable variability exists in HB vaccine responses, with 5-10% of healthy Japanese adults demonstrating no response following a standard vaccination. Recently, polymorphisms of immune-regulatory genes, such as cytokine genes, have been reported to influence the immune response to HB vaccine. The aim of this study was to investigate the underlying mechanisms of the genetic association between several cytokine gene polymorphisms and the immune response to HB vaccination in a Japanese population. One hundred and twenty three vaccinated young adults were classified according to the level of antibody-titer (anti-HBs). Single nucleotide polymorphism typing for IFN-γ (+874, 3'-UTR), IL-10 (-591, -819, -1082), and TNF-α (-308, -857), was accomplished using the PCR-RFLP or SSP-PCR method. The TNF-α (-857) CC type and the IL-10 (-1082) AG type were present more frequently in the low titer group than in the high titer group. The TNF-α (-857) CC type was found to be significantly associated with low response of serum anti-HBs. The anti-HBs antibody was not readily produced in the IL-10 (-1082) AG and TNF-α (-857) CC haplotype. Conversely, the antibody was readily produced in the IL-10 (-1082) AA and TNF-α (-857) CC haplotype, and the IL-10 (-1082) AA and TNF-α (-857) CT haplotype, suggesting a high likelihood of the IL-10 (-1082) AG type to be included in the low anti-HBs group, and high anti-HBs antibody production in those with the TNF-α (-857) CT type. These SNPs may produce ethnically-specific differences in the immune response to HB vaccine in the Japanese population. J. Med. Invest. 63: 256-261, August, 2016.

  5. Validación de un ensayo ELISA para la determinación de anticuerpos anti LPS de Vibrio Cholerae

    Directory of Open Access Journals (Sweden)

    Yadira Pino

    2003-03-01

    Full Text Available Para evaluar la respuesta inmunológica de cualquier candidato vacunal es necesario contar en el laboratorio con técnicas estandarizadas y validadas. Es por ello que en este trabajo se realizaron los ensayos para la estandarización del ELISA indirecto, usado para la determinación de anticuerpos séricos anti-LPS Ogawa contra cólera en suero de humanos inoculados por vía oral, así como la evaluación de los parámetros de validación típicos de este ensayo. Para la selección de las condiciones óptimas se evaluaron la concentración de recubrimiento, condiciones de bloqueo y dilución de conjugado, con el objetivo de seleccionar las mejores respuestas para el control positivo, el control negativo y el blanco en cada caso.Una concentración de 25 μg/mL de LPS Ogawa en PBS, como recubrimiento, durante toda la noche; leche descremada al 0,5% en PBS, 30 min a temperatura ambiente como bloqueo y el conjugado anti-IgA-HRP diluido 1:5000, resultaron las variables óptimas para el ensayo. En cuanto a la validación de la técnica los parámetros evaluados fueron precisión, exactitud, límite de detección, especificidad y robustez. Para ello se siguió un protocolo de validación que permitiera evaluarlos, y en todos los casos el ensayo se consideró adecuado, siempre y cuando el coeficiente de variación resultara ser menor del 20%. En todos los parámetros evaluados se cumplió con este requisito, considerando así los resultados confiables y reproducibles.

  6. Anti-HBs seroconversion during treatment with entecavir in a patient with chronic hepatitis B virus infection on hemodialysis.

    Science.gov (United States)

    Spaziante, Martina; Biliotti, Elisa; Grieco, Stefania; Palazzo, Donatella; Esvan, Rozenn; Taliani, Gloria

    2014-01-01

    Hepatitis B (HBV) virus infection is one of the most important causes of liver disease in patients with end-stage renal failure on hemodialysis. The natural history of chronic HBV infection acquired in childhood starts with an immune tolerant phase, followed by an immune clearance phase that may lead to the inactive carrier state or the development of chronic liver disease. Information on antiviral therapy administered very early during the immune clearance phase are lacking and no data exist on the treatment of early immune activation in the hemodialysis setting. This report describes the case of a patient affected by end-stage renal failure and HBeAg-positive chronic HBV virus infection treated very early during the immune clearance phase of HBV infection with an adjusted-dose of nucleoside analogue entecavir. The patient achieved a very rapid HBV-DNA undetectability, anti-HBe, and anti-HBs seroconversion. This is the first report of antiviral therapy with entecavir started during the immune reactive phase of HBV infection in a patient on hemodialysis and it suggests that antiviral treatment can enhance the effects of host immune activation resulting in biochemical, serological, and viral response, even in end-stage renal failure patients with partial immunodeficiency. Antiviral therapy with entecavir in the setting of hemodialysis was safe and well tolerated.

  7. Coexistence of hepatitis B surface antigen and anti-HBs in Chinese chronic hepatitis B virus patients relating to genotype C and mutations in the S and P gene reverse transcriptase region.

    Science.gov (United States)

    Liu, Weiwei; Hu, Tingting; Wang, Xinyu; Chen, Yuming; Huang, Minying; Yuan, Chao; Guan, Ming

    2012-04-01

    We aimed to determine the prevalence of the coexistence of HBsAg and anti-HBs and to analyze the clinical and virological features of infection, including amino acid (aa) patterns of the S gene and reverse transcriptase (RT) region in Chinese chronic hepatitis B (CHB) patients. Fifty-four (2.90%) CHB patients who were positive for both HBsAg and anti-HBs were tested, and sequences were obtained from 52 of them as well as 48 patients from a control group. S gene and RT region sequences were amplified and sequenced using in-house protocols. There was no significant difference between patients with and without anti-HBs with regard to age, gender, alanine aminotransferase level, and the proportion positive for HBeAg and HBcAb. The occurrence of genotype C (P = 0.001) and anti-HBeAb positivity (P = 0.027) was significantly higher in HBsAg+/anti-HBs+ individuals. In the S gene, the number of mutated residues in the HBsAg+/anti-HBs+ group was markedly higher than in control patients (1.88 versus 1.02 substitutions per 100 amino acids, P = 0.022). The amino acid exchange occurred mostly within the N-terminal region (2.15 versus 0.87 substitutions per 100 amino acids, P = 0.023) and the "a" determinant (3.61 versus 1.56 substitutions per 100 amino acids, P = 0.049) in the two groups. In the RT region, the mean number of substitution per 100 aa showed a tendency to be significantly higher in HBsAg+/anti-HBs+ patients than in controls (2.34 versus 1.46, P = 0.040). This study showed a prevalence of coexistence of anti-HBs in HBsAg-positive patients and an increased frequency of genotype C and aa variability within both HBsAg and RT involving functionally important regions of those proteins.

  8. 乙型肝炎病毒表面抗原和表面抗体双阳性者S蛋白序列分析%Mutations of S protein gene in positive HBsAg and anti-HBs patients with chronic hepatitis B virus infection

    Institute of Scientific and Technical Information of China (English)

    丁锋; 于红刚; 李艳霞; 戴金芬; 慕刚刚

    2015-01-01

    Objective To investigate mutations of S protein gene in positive HBsAg and anti-HBs patients with chronic hepatitis B virus (HBV) infection.Methods Fifteen HBsAg(+) and anti-HBs(+) patients and 22 HBsAg(+) and anti-HBs (-) patients (control group) admitted in Renmin Hospital of Wuhan University during January and December 2011 were enrolled in the study.The S protein gene was amplified and sequenced, and the amino acid sequences were translated from the obtained DNA sequences and compared with the reference sequences.Results Compared with the control group, HBsAg (+) and anti-HBs(+) patients showed a higher variability in amino acid within major hydrophilic region (2.95 vs.0.78,x2 =18.059, P<0.01) and the "a" determinant (4.44 vs.1.52, x2 =6.985, P<0.01).The mutations in "a" determinant at positions P127T, G130E, G130N, M133S, F134I, T140I and G145R were detected only in HBsAg(+) and anti-HBs (+) patients.Conclusion Co-existence of HBsAg and anti-HBs in patients with chronic HBV infection might be associated with the increased amino acid mutations in and around the "a" determinant of protein S.%目的 探讨慢性乙型肝炎病毒(HBV)感染者乙型肝炎表面抗原(HBsAg)和乙型肝炎表面抗体(抗-HBs)双阳性与S蛋白氨基酸突变的关系.方法 连续收集2011年1至12月武汉大学人民医院HBsAg(+)且抗-HBs(+)慢性HBV感染患者15例作为观察组,选取22例HBsAg(+)且抗-HBs(-)慢性HBV感染患者作为对照组.对两组患者HBV DNA S基因序列进行扩增和测序,将测序结果与标准序列比对并翻译为氨基酸序列,将两组的氨基酸突变率进行对比分析.结果 观察组在主要亲水区(2.95 vs.0.78,x2=18.059,P<0.01)、"a"决定簇(4.44vs.1.52,x2 =6.985,P<0.01)氨基酸突变率高于对照组.仅有观察组出现"a"决定簇内氨基酸突变:P127T、G130E、G130N、M133S、F134I、T140I和G145R.结论 HBsAg和抗-HBs共存可能与S蛋白"a"决定簇及其周围区域的高氨基酸突变有关.

  9. Long-term anti-HBs antibody persistence and immune memory in children and adolescents who received routine childhood hepatitis B vaccination.

    Science.gov (United States)

    Behre, Ulrich; Bleckmann, Gerhard; Crasta, Priya Diana; Leyssen, Maarten; Messier, Marc; Jacquet, Jeanne-Marie; Hardt, Karin

    2012-06-01

    This paper presents data from two studies that evaluated 5-y and 10-y persistence of antibodies against hepatitis B (HBV) surface antigen (anti-HBs) and immune response to an HBV vaccine challenge in children and adolescents who had received three doses of a HBV vaccine in infancy as part of routine clinical practice [NCT00519649/NCT00984139]. Anti-HBs antibody concentrations ≥ 10 mIU/ml persisted in 83.3% (95% confidence interval [CI]: 78.5–87.5) and 78.3% (95% CI: 73.1–83.0) of subjects aged 7–8 y and 12–13 y, respectively 5–10 y after infant vaccination. One month postchallenge dose, 98.2% (95% CI: 95.9–99.4) and 93.7% (95% CI: 90.2–96.2) of subjects in the two age groups, respectively had anti-HBs antibody concentrations ≥ 100 mIU/ml. Overall, 99.6% (95% CI: 98–100) and 97.2% (95% CI: 94.5–98.8) of subjects aged 7–8 y and 12–13 y mounted an anamnestic response to the HBV challenge dose, which was well-tolerated. Healthy children aged 7–8 y and adolescents aged 12–13 y received three doses of a monovalent pediatric HBV vaccine (10 μg of HBsAg) before 18 mo of age. Serum samples collected before and one month post-HBV vaccine challenge dose were tested for anti-HBs antibody concentrations. Safety assessments were made for the HBV vaccine challenge dose. A three-dose childhood HBV immunization regimen induced persistence of antibodies against HBV infection for 10 y, up to adolescence. This vaccination regimen also conferred long-term immune memory against HBV as evidenced by the strong anamnestic response to the HBV vaccine challenge, despite waning anti-HBs antibody levels.

  10. The effects of different dosage levels of hepatitis B vaccine as booster on anti-HBs-negative children 5-15 y after primary immunization; China, 2009-2010.

    Science.gov (United States)

    Chen, Yongdi; Lv, Huakun; Gu, Hua; Cui, Fujiang; Wang, Fuzhen; Yao, Jun; Xia, Shichang; Liang, Xiaofeng

    2014-01-01

    The changes in lgG antibody levels to hepatitis B surface antigen (HBsAg) and in antibody to HBsAg (anti-HBs) seroconversion rates due to different dosages of hepatitis B vaccine (HepB) were compared in 2106 children. Children who had been previously vaccinated as infants with HepB were revaccinated at 5-15 y of age, after which the antibody titers were determined. After the first booster dose, the anti-HBs seroconversion rate (defined as an anti-HBs ≥10 mIU/ml) with 10 μg of HepB (93.6%) was significantly greater than the rate with 5 µg of HepB (90.3%) (Panti-HBs seroconversion rate in 10-15-y-old boys vaccinated with 10 μg of HepB (90.9%) was significantly greater than the rate with 5 µg of HepB (84.3%) (Panti-HBs seroconversion rates after the third booster dose with 5 or 10 μg of HepB were greater than those after the first booster dose (99.6% and 99.7%, vs. 90.3% and 93.6%, Panti-HBs-negative children after a single dose of booster, 3 doses are needed.

  11. Deficit de proteina Z de la coagulación y anticuerpos anti-proteina Z como factores de riesgo de recidiva trombotica arterial y venosa. Estudio prospectivo a largo plazo

    OpenAIRE

    Pardos Gea, José

    2016-01-01

    Antecedentes: La proteína Z (PZ) es un cofactor de la coagulación que unido a su proteasa activa (ZPI) forman el ultimo sistema anticoagulante descrito. Los modelos animales confirman que el déficit de PZ es trombofílico. El número y calidad de los estudios clínicos ha sido insuficiente para constatar este hecho. Los anticuerpos anti-PZ descritos en patología obstétrica nunca se habían testado en patología trombótica vascular. Objetivos: El objetivo inicial fue investigar si el déficit de PZ ...

  12. Buldan Göğüs Hastalıkları Hastanesi’ne Başvuran Hastalarda HBsAg ve Anti HBs Seropozitifliği

    OpenAIRE

    DEDE, Ayşe; ÇALIŞKAN, Emel

    2015-01-01

    Hepatit B Virüsü hepatite en sık neden olan virüslerden olup, ciddi klinik tablolara neden olabilmektedir. Günümüzde yenidoğan döneminde başlayan aşılama çalışmaları ile hastalığın bulaşı önlenmeye çalışılmaktadır. Bu çalışmada, ilçemizdeki Hepatit B Virüsü ile karşılaşma sıklığının ve yaş aralığının belirlenmesi amaçlanmıştır. Buldan Göğüs Hastalıkları Hastanesi’ne Ocak 2012-Ocak 2013 tarihleri arasında çeşitli nedenlerle başvuran hastalardaki HBsAg ve AntiHBs pozitiflikleri retrospektif ola...

  13. The effects of booster vaccination on combined hepatitis A and hepatitis B vaccine in both anti-HBs and anti-HAV negative children 5-15 years after hepatitis B vaccine primary immunization.

    Science.gov (United States)

    Chen, Yongdi; Gu, Hua; Cheng, Suyun; Shen, Lingzhi; Cui, Fujiang; Wang, Fuzhen; Yao, Jun; Xia, Shichang; Lv, Huakun; Liang, Xiaofeng

    2013-04-01

    In the present study, we investigated the changes in both anti-HAV lgG and anti-HBs lgG levels and compared the antibody seroconversion rates of different doses of combined hepatitis A and hepatitis B vaccine in children. Children who were vaccinated as infants with Hepatitis B vaccine were revaccinated at 5-15 y of age, then the antibody titers were monitored. Among 283 children, this study found that the anti-HAV seroconversion rates (defined as anti-HAV ≥ 1 mIU/ml) after the first and the third dose were 79.9% and 100% respectively; these observed differences were statistically significant (Panti-HBs seroconversion rates (defined as an anti-HBs ≥ 10 mIU/ml) were 82.3% and 99.0% respectively; these observed differences were statistically significant (Panti-HAV seroconversion rate was statistically significant in children aged 5-9 y and 10-15 y (Panti-HBs seroconversion, whereas after the third dose the difference was not statistically significant (P>0.05). This study demonstrated that the immunization effects of booster vaccination with combined hepatitis A and hepatitis B vaccine is successful for children. A single booster dose is adequate for younger children, while three doses are needed for older children.

  14. Los niveles de anticuerpos anti factor plaquetario 4-heparina y el índice 4T para trombocitopenia inducida por heparina

    Directory of Open Access Journals (Sweden)

    Marta E. Martinuzzo

    2012-02-01

    Full Text Available La trombocitopenia inducida por heparina (HIT es un efecto adverso del tratamiento con heparina, mediada por anticuerpos anti complejo factor plaquetario 4 (PF4-heparina (HPIA. La HIT es frecuentemente moderada pero pueden desarrollarse complicaciones trombóticas. El diagnóstico precoz es importante. La detección de HPIA por ELISA tiene alta sensibilidad pero baja especificidad (títulos bajos sin significación clínica. El índice de las 4T (índice 4T puede detectar pacientes con alto riesgo de HIT. El propósito del estudio fue correlacionar los niveles de HPIA y el índice 4T de un grupo de pacientes derivados a nuestro centro. Evaluamos 84 pacientes, 34 de ellos desarrollaron trombosis. Cada médico completó un cuestionario clínico que fue remitido con la muestra a nuestro centro. Los cuestionarios fueron analizados por un investigador externo y el índice 4T se calculó previamente al ensayo. Los HPIA se determinaron por un ELISA (Asserachrom HPIA que detecta los 3 isotipos, IgG, IgM e IgA, único reactivo disponible en Argentina. Los resultados se expresaron como porcentaje de absorbancia (%ABS. La correlación del índice 4T con los HPIA fue 0.472 (rho spearman, p < 0.001. Los pacientes con índice 4T ≥ 6 presentaban %ABS mayores que los ≤ 5 (67 vs. 39, p < 0.001. Aquéllos con trombosis presentaron títulos mayores que los que no la desarrollaron (%ABS 59 vs. 39, p = 0.017. En conclusión: Los títulos altos de HPIA medidos por ELISA, que detecta los 3 isotipos, correlacionaron claramente con el índice 4T ≥ 6 y fueron más frecuentes en los pacientes con trombosis, coincidiendo con lo ya descripto para ensayos de ELISA específicos para isotipo IgG.

  15. Efecto de la vacunación anti-leptospira en bovinos lecheros sobre la producción de anticuerpos anti-leptospira en trabajadores de lecherías de cuatro zonas de Costa Rica

    Directory of Open Access Journals (Sweden)

    Rafael González

    2007-07-01

    Full Text Available Objetivo: Este estudio experimental midió el efecto indirecto de la vacuna anti-Leptospira Leptoferm-5® (Pfizer® en la producción de anticuerpos anti-Leptospira en trabajadores de lecherías de cuatro zonas lecheras de Costa Rica. Materiales y Métodos: Tomaron parte en el estudio 171 vaqueros, 94 en el grupo tratamiento y 77 en el grupo control. A cada vaquero se le tomó una muestra sanguínea en tres diferentes momentos durante un año: la primera, antes de aplicar la vacuna en los animales, la segunda a los 6 meses y la tercera un año después de la primera vacunación. Se utilizó la técnica de micro aglutinación para la detección de anticuerpos anti-Leptospira interrogans serovares pomona, hardjo, canícola y grippotyphosa en el laboratorio del INCIENSA. Se utilizó la razón de prevalencias y el riesgo relativo para determinar si existió asociación entre la vacunación anti-Leptospira a las vacas y la seropositividad en los vaqueros. Resultados: En los tres muestreos se obtuvo un 10.27% (50/487 de muestras positivas a cualquiera de los serovares de Leptospira interrogans a los que fueron analizados. Tanto en el grupo tratamiento como en el control se observaron cambios en la seropositividad entre los distintos muestreos, con un aumento entre el primer y segundo muestreo (p Objective: This experimental study assessed the indirect effect of the anti-Leptospira vaccine Leptoferm-5® (Pfizer® regarding the production of anti-Leptospira antibodies in dairymen from four dairy areas in Costa Rica. Materials and Methods: 171 dairymen took part in the study; 94 in the treatment group and 77 in the control group. Three blood samples were taken from each worker three times throughout a year period: before the vaccination of the animals, and six and twelve months after the initial vaccination. The microscopic agglutination test (MAT was used to detect anti-Leptospira interrogans antibodies, specifically towards pomona, hardjo, canicola

  16. 重庆市2008~2012年无偿献血者HBsAg,ALT及抗HIV、抗HCV、抗TP抗体检测结果的分析%A survey on test results of HBsAg,ALT and anti-HIV,anti-HCV,anti-TP antibodies among voluntary blood donors in Chongqing from 2008 to 2012

    Institute of Scientific and Technical Information of China (English)

    程颖; 李维; 程燃

    2014-01-01

    目的:分析重庆市2008~2012年无偿献血者乙型肝炎病毒表面抗原(HBsAg),丙氨酸氨基转移酶(ALT)及抗丙型肝炎病毒(HCV)、抗人类免疫缺陷病毒(HIV)、抗梅毒螺旋体(TP)抗体检测结果,为招募低危无偿献血者,减少血液报废提供依据。方法选择2008~2012年于重庆市血液中心接受酶联免疫吸附测定(ELISA)筛查的无偿献血者的血液标本551133例,检测其HBsAg ,ALT及抗HIV、抗HCV、抗TP抗体。采用实验室信息管理系统ITSWELL对检测结果进行读取、保存、汇总,并判断标本是否合格。结果共计检出不合格标本37534例(6.81%),其中,HBsAg ,ALT 及抗 HCV、HIV、TP抗体不合格率分别为1.10%、3.79%、0.51%、0.33%、1.08%。结论加强血液检测试剂筛选及无偿献血者队伍的建设将有助于临床的输血安全。%Objective To analyze the test results of hepatitis B virus surface antigen(HBsAg) ,alanine aminotransferase(ALT) and anti-hepatitis C virus (HCV) ,anti-human immunodeficiency virus (HIV) ,anti-treponema pallidum (TP) antibodies among voluntary blood donors ,and to provide basis for recruiting low-risk voluntary blood donors and reducing blood abandonment . Methods 551 133 blood samples derived from voluntary blood donors which had accepted enzyme-linked immunosorbent assay (ELISA) screening in Chongqing Blood Center from 2008 to 2012 were collected and were subject to HBsAg ,ALT and anti-HIV , anti-HCV ,anti-TP antibodies detection .Laboratory information management system ITSWELL was employed to read ,save and gather the test results ,and whether the samples were qualified was determined .Results Total of 37 534(6 .81% ) substandard blood samples were detected .Among them ,the substandard rates of HBsAg ,ALT and anti-HCV ,anti-HIV ,anti-TP antibodies were 1 .10% ,3 .79% ,0 .51% ,0 .33% and 1 .08% ,respectively .Conclusion Strengthening the screen of

  17. [The management of dialysis patients seropositive for HBsAg, anti-HCV, or anti-HIV antibodies].

    Science.gov (United States)

    Fabrizi, Fabrizio; Messa, Piergiorgio

    2012-01-01

    Infections by hepatitis B or hepatitis C virus are still common among patients on maintenance dialysis in Western countries. The natural history of HBV and HCV in the dialysis population remains unclear; however, there is good evidence showing an adverse impact of an anti-HCV seropositive status on survival in dialysis patients. A recent meta-analysis of observational studies (n=7, 11,589 unique patients) reported that anti-HCV-positive patients on dialysis had a higher mortality rate than those who were anti-HCV negative (adjusted hazard ratio=1.35, 95% confidence interval, 1.13; 1.59, pdialysis. Standard precautions and specific procedures against the transmission of blood-borne agents have been recommended to control HCV infection within dialysis units. Isolation by dialysis machines, staff and rooms has been strongly recommended to control HBV. Vaccination is an important tool against transmission of HBV infection among patients on maintenance dialysis; however, the immune response towards the hepatitis B vaccine in uremic patients remains unsatisfactory. Monotherapy with lamivudine is currently used for dialysis patients with hepatitis B whereas combination antiviral therapy (pegylated interferon plus ribavirin) is the standard of care for hepatitis C in the dialysis population, even if various side effects have been observed.

  18. Toxocara seroprevalence in children from a subtropical city in Argentina Prevalencia de anticuerpos anti Toxocara en niños de una ciudad subtropical de Argentina

    Directory of Open Access Journals (Sweden)

    José Mario ALONSO

    2000-08-01

    Full Text Available Most studies from Argentina have focused on toxocariasis as an environmental problem of big cities, and there are no available data about children infection from small or middle-sized cities. In order to assess the prevalence of anti-Toxocara antibodies in infantile population, 206 children from Resistencia, of both sexes, aged 1-14 years old were studied by Elisa testing with E/S T. canis L2 antigens. Hematological parameters and immunoglobulin levels were determined; five days' stool samples were studied and epidemiological data were obtained by means of a questionnaire to parents. Results showed that 73% of the children had one or more dogs living at home, 57% reported geophagia and 37.9% were positive for Toxocara serology, but there was no significant difference in prevalence neither for boys and girls, nor concerning age. An increased risk of infection was observed in age groups 5-6 and 7-8 for boys, and in age groups 3-4 and 5-6 for girls.La mayoría de los estudios realizados en Argentina, han enfocado a la Toxocariosis como un problema medioambiental propio de las grandes ciudades y no existen datos acerca de esta infección entre los niños de ciudades medianas o pequeñas. A fin de determinar la prevalencia de anticuerpos anti-Toxocara en la población infantil de la ciudad de Resistencia, se estudiaron 206 niños de ambos sexos, con edades comprendidas entre los 1-14 años, mediante el test de Elisa, empleando antígenos E/S de larva L2 de Toxocara canis. También se evaluaron los niveles de inmunoglobulinas séricas y los parámetros hematológicos; se efectuaron análisis coproparasitológico seriados en heces de 5 días y se recolectó información epidemiológica mediante cuestionario a los padres. Los resultados obtenidos señalan que el 73% de los niños tenían uno o mas perros viviendo en la casa, que el 57% tenían antecedentes de geofagia y que el 37.9% tenían serología positiva para Toxocara, aunque no hubo diferencias

  19. El potencial de la inmunomodulación con anticuerpos monoclonales anti-CD137 (4-1BB para terapia de enfermedades malignas e infecciones virales crónicas The immunotherapy potential of agonistic anti-CD137 (4-1BB monoclonal antibodies for malignancies and chronic viral diseases

    Directory of Open Access Journals (Sweden)

    C. Alfaro

    2006-04-01

    Full Text Available La manipulación farmacológica del sistema inmunitario para conseguir respuestas linfocitarias de mayor intensidad tiene aplicación potencial en inmunoterapia tumoral y en el tratamiento de enfermedades virales crónicas. Los anticuerpos monoclonales inmunoestimuladores se definen como una familia de fármacos que aumentan la respuesta inmunitaria al interaccionar como ligandos artificiales con proteínas funcionales del sistema inmunitario, activando o inhibiendo su función. Hay anticuerpos monoclonales humanizados dirigidos frente al receptor inhibidor linfocitario CD152 (CTLA-4 que se están probando en ensayos clínicos con evidencia de actividad antitumoral, aunque con la contrapartida de producir reacciones autoinmunitarias severas. Los anticuerpos anti-CD137 tienen la capacidad de inducir potentes respuestas inmunitarias, mediadas principalmente por linfocitos T citotóxicos, con el resultado de erradicar tumores transplantables de ratón de forma comparativamente superior a los anticuerpos frente a CD152. CD137 (4-1BB es un antígeno de diferenciación expresado selectivamente en la superficie de linfocitos T y NK activados y sobre células dendríticas. Los anticuerpos monoclonales que actúan como ligandos artificiales estimuladores de este receptor (anticuerpos monoclonales agonistas anti-CD137 potencian la inmunidad celular antitumoral y antiviral en modelos experimentales murinos. Paradójicamente, estos mismos anticuerpos previenen o mejoran el curso de enfermedades autoinmunitarias establecidas en ratones como modelo. A la luz de estos datos experimentales, varios grupos de investigación han procedido a la humanización de anticuerpos dirigidos frente a CD137 humano y se plantea la inminente realización de los primeros ensayos clínicos.Pharmacological intervention on the immune system to achieve more intense lymphocyte responses has potential application in tumour immunology and in the treatment of chronic viral diseases

  20. Unusual Naturally Occurring Humoral and Cellular Mutated Epitopes of Hepatitis B Virus in a Chronically Infected Argentine Patient with Anti-HBs Antibodies

    Science.gov (United States)

    Cuestas, María L.; Mathet, Verónica L.; Ruiz, Vanesa; Minassian, María L.; Rivero, Cintia; Sala, Andrea; Corach, Daniel; Alessio, Analía; Pozzati, Marcia; Frider, Bernardo; Oubiña, José R.

    2006-01-01

    Serum hepatitis B virus (HBV) DNA was extracted from a chronically infected patient with cocirculation of hepatitis B surface antigen (HBsAg) and anti-HBs antibodies. Direct PCR and clone-derived sequences of the S and overlapped P genes were obtained. DNA sequences and phylogenetic analysis ascribed this isolate to genotype A (serotype adw2). Five of six HBV DNA clones exhibited point mutations inside and outside the major hydrophilic region, while the sixth clone exhibited a genotype A “wild-type” amino acid sequence. Observed replacements included both humoral and/or cellular (major histocompatibility complex class I [MHC-I] and MHC-II) HBV mutated epitopes, such as S45A, P46H, L49H, C107R, T125A, M133K, I152F, P153T, T161S, G185E, A194T, G202R, and I213L. None of these mutants were individually present within a given clone. The I213L replacement was the only one observed in the five clones carrying nonsynonymous mutations in the S gene. Some of the amino acid substitutions are reportedly known to be responsible for the emergence of immune escape mutants. C107R replacement prevents disulfide bonding, thus disrupting the first loop of the HBsAg. Circulation of some of these mutants may represent a potential risk for the community, since neither current hepatitis B vaccines nor hyperimmune hepatitis B immune globulin are effectively prevent the liver disease thereto associated. Moreover, some of the recorded HBsAg variants may influence the accuracy of the results obtained with currently used diagnostic tests. PMID:16757620

  1. Infección oculta por el virus de la hepatitis B en hijos de madres positivas al HBsAg

    Directory of Open Access Journals (Sweden)

    Marité Bello-Corredor

    2016-04-01

    Full Text Available La infección oculta por el virus de la hepatitis B (IOB, se caracteriza por la presencia en suero o plasma del genoma viral (ADN-VHB y anticuerpos contra la proteína de la cápside (anti-HBc en ausencia del antígeno de superficie (HBsAg, marcador que tradicionalmente se emplea para identificar la presencia del virus. Con el objetivo de caracterizar la presencia de IOB en hijos de madres positivas al HBsAg, se estudiaron 291 muestras séricas de niños con la condición de ser HBsAg (- y anticuerpos anti-HBsAg (anti-HBs menores de 50 UI/L, conservadas en la seroteca del Laboratorio de Referencia Nacional de Hepatitis Virales. Se realizaron ensayos para determinar la exposición al virus (anti-HBc, a los sueros anti-HBc (+ se les realizó Reacción en Cadena de la Polimerasa en Tiempo Real (RCP-TR para determinar y cuantificar el ADN-VHB. La prevalencia de exposición al VHB (anti-HBc fue 16,8% (49/291. El ADN viral se cuantificó en el 14% (6/43 de los casos anti-HBc (+, observándose cargas virales que oscilaban entre 2,15 x 101 hasta 3,42 x 101 UI/mL. La prevalencia de la IOB para el total de los pacientes analizados fue 2,1% (6/291, considerada relativamente baja. No se encontró asociación significativa entre las variables sociodemográficas analizadas tales como: edad, sexo y provincia de procedencia. La IOB está presente en hijos de madres positivas al HBsAg, a pesar de la profilaxis contra la hepatitis B. Por lo tanto, se requiere de pesquisajes adecuados para detectar dicha entidad. Las implicaciones clínicas y epidemiológicas de la misma, requieren de un estrecho monitoreo y atención de estos pacientes. Este estudio se realiza por primera vez en Cuba y aporta conocimientos útiles para el diagnóstico, prevención y control de esta enfermedad en niños.

  2. 非竞争性ELISA法测定人源抗HBsAg Fab功能性亲和常数%Measurement of functional affinity of humanized anti-HBsAg Fab using non-competitive ELISA method

    Institute of Scientific and Technical Information of China (English)

    郑大勇; 罗荣城; 韩焕兴

    2004-01-01

    目的测定完全人源化基因工程抗体HBsAg Fab的亲和常数.方法采用非竞争性ELISA固相法,经确定最佳抗原包板浓度、最佳抗原包板时间及最佳抗原与抗体结合反应时间后,得到了HBsAg与抗体片段抗HBsAg Fab及完整抗体抗HBsAg IgG的抗原抗体结合反应曲线,计算出抗HBsAg Fab及抗HBsAg IgG的亲和常数.结果人源基因工程抗体抗HBsAg Fab的功能性亲和常数在107~108M-1水平,比完整抗HBsAg IgG仅仅小约1个数量级(108~109M-1).结论该基因工程抗体与抗原结合能力较强,为今后开发应用Fab进行生物导向治疗提供了理论基础.

  3. Prevalencia de anticuerpos anti-Leptospira spp. en personas con exposición laboral en el departamento del Tolima / Prevalence of anti-Leptospira spp. antibodies among people with occupational exposure in Tolima Department

    Directory of Open Access Journals (Sweden)

    Blanca L. Guzmán-Barragán

    2016-05-01

    Full Text Available Resumen Objetivo: estimar la prevalencia de anticuerpos IgM contra Leptospira spp., mediante el Ensayo de Inmunoabsorción Ligado a Enzimas (elisa, en la población de riesgo laboral de 8 municipios del Tolima. Metodología: se obtuvieron muestras de sangre de 261 empleados, las cuales fueron analizadas mediante la técnica de elisa para la detección de anticuerpos IgM anti-Leptospira spp., seguido de mat y serotipificación. Resultado: se estimó una seroprevalencia del 25,29%, con una seroreactividad mayor en trabajadores de plantas de beneficio animal (34,2%, recolección de residuos sólidos (27,1% y trabajadores de acueducto y alcantarillado (14,8%. La actividad en plantas de beneficio animal se identificó como factor de riesgo de Leptospira spp. (OR=1,86. Los serovares identificados fueron L. Bratislava (16, Ballum (5, Tarassovi (3, Hebdomadis (2, Sejroe (2 y Icterhemorragiae (1. El municipio de Libano presento el mayor porcentaje de positividad (36,96%, seguido de Espinal y Guamo con 28,57% cada uno. Discusión: la evaluación del sistema de vigilancia indicó deficiencia en recursos y debilidades de los profesionales de la salud al desconocer los procedimientos, investigación, diagnóstico y notificación de la enfermedad. Conclusiones: la leptospirosis está presente en poblaciones de riesgo laboral en el Tolima y se hace necesario abordar esta problemática en la población de otros municipios y los animales transmisores de la enfermedad. / Abstract Objective: to estimate the prevalence of IgM antibodies against Leptospira spp. Using the enzyme-linked immunosorbent assay (elisa in a population at occupational risk from 8 municipalities of the Tolima department, Colombia. Methodology: blood samples were collected from 261 employees and analyzed with the elisa technique to detect IgM and anti-Leptospira spp. antibodies. This was followed by mat and serotyping. Results: a seroprevalence of 25.29% was estimated, with higher

  4. Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance Caracterização de uma cepa de hepatite por vírus B no sudoeste do Paraná, Brasil, apresentando mutações previamente associadas à resistência anti-HBs

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    Dennis Armando Bertolini

    2010-02-01

    Full Text Available The present study investigated if hepatitis B virus (HBV mutants circulate in the southwestern region of the State of Paraná, Brazil, by analyzing samples from children who received immunoprophylaxis but were born to HBV carrier mothers. Samples from 25 children were screened for HBV serum markers and for HBV DNA by PCR. Only one sample was positive for HBsAg, anti-HBs and HBV DNA, although the child had been vaccinated. Analysis of the S gene sequence of this sample showed the presence of a proline at position 105, a serine at position 114, three threonines at positions 115, 116 and 140, and a glutamine at position 129. The presence of these amino acids, except for serine at position 114, has been related to monoclonal or polyclonal therapy with anti-HBs after liver transplantation, whereas the presence of threonine at position 116 has been described in immunized children from Singapore. This finding demonstrates the possible circulation of HBV strains resistant to hepatitis B immunoprophylaxis in southwestern Paraná, Brazil. The genotype of the sample was identified as genotype D, which is frequently found in the region studied. Since 36% of the children had received incomplete or no immunoprophylaxis, more extensive follow-up of children born to HBsAg-positive mothers is needed.O presente estudo investigou se mutantes do vírus da hepatite B (HBV circulam na região Sudoeste do Estado do Paraná, Brasil, analisando amostras de crianças que receberam a imunoprofilaxia por terem nascido de mães portadoras do HBV. Amostras de 25 crianças foram analisadas para os marcadores sorológicos do HBV e para o DNA-HBV por PCR. Somente uma amostra foi positiva para AgHBs, anti-HBs e DNA-HBV, apesar da criança ter sido vacinada. Análises da seqüência do gene S desta amostra mostrou a presença de uma prolina na posição 105, uma serina na posição 114, três treoninas nas posições 115, 116 e 140, e uma glutamina na posição 129. A presen

  5. Measurements in international units of antibody to hepatitis B surface antigen(anti-HBs) after immunization with a yeast-derived, subtype adr hepatitis B vaccine are considerably different between chemiluminescent immunoassay (CLIA) and chemiluminescent enzyme immunoassay (CLEIA).

    Science.gov (United States)

    Ogata, Norio

    2006-04-01

    The worldwide consensus of the minimum protective anti-HBs level against HBV infection is 10 mIU/mL on assays standardized by the World Health Organization (WHO) reference preparations. To investigate whether this value could be applied to recipients of yeast-derived recombinant HB vaccine containing the major surface protein of subtype adr (Bimmugen, Astellas Pharmaceutical, Tokyo), we compared anti-HBs measurements between chemiluminescent immunoassay (CLIA) (Architect Ausab, Abbott Japan, Tokyo) and chemiluminescent enzyme immunoassay (CLEIA) (Lumipulse Forte, Fujirebio, Tokyo) in given serum samples obtained from the vaccinees. The vaccine and the two assay methods are currently in a wide use in Japan. The study included 300 medical students who completed a standard vaccination course (0, 1 and 6 months). Serum samples obtained 1 month or 13 months after completing the vaccination were simultaneously tested for anti-HBs by CLIA and CLEIA. In 147 samples with quantifiable values on both CLIA and CLEIA (10 to 1000 mIU/mL) the geometric mean titer on CLEIA (225.0 mIU/mL) was significantly higher than that on CLIA (94.5 mIU/mL) (p anti-HBs measurements compared to CLIA and discordance in determining critical anti-HBs level of 10 mIU/mL was observed in more than half the samples. This suggests that the minimum HBV-protective anti HBs titer of 10 mIU/mL is difficult to be introduced to Japan where subtype adr-HB vaccines or -HBV infection are prevalent, unless characteristics of assay methods are carefully evaluated.

  6. Anti-HBs levels among children and adolescents with complete immunization schedule against hepatitis B virus. A cross-sectional study in Blumenau, State of Santa Catarina, Brazil, 2007-2008 Níveis de anti-HBs entre crianças e adolescentes com o esquema completo de imunização contra o vírus da hepatite B. Um estudo transversal em Blumenau, Estado de Santa Catarina, 2007-2008

    Directory of Open Access Journals (Sweden)

    Andréa do Livramento

    2011-08-01

    Full Text Available INTRODUCTION: Vaccination is the main tool for preventing hepatitis B virus (HBV infection; however, following the completion of the vaccination series, the concentrations of anti-HBs can decline over the years and reach levels less than 10mIU/mL. The persistence of protection in these individuals is still unknown. The present study aimed to determine the anti-HBs antibody levels among children and adolescents who had received a complete vaccination course for hepatitis B. METHODS: Antibodies against HBV surface antigen (anti-HBs were tested in 371 individuals aged 10 to 15 years-old. RESULTS: Volunteers who showed undetectable quantities of anti-HBs accounted for 10.2% of the population studied and 39.9% presented antibody titers of less than 10mIU/mL. Anti-HBs > 10mIU/mL were verified in 49.9%. CONCLUSIONS: These results corroborate other studies indicating levels of anti-HBs below 10mIU/mL in vaccinated individuals. Additional studies are required to assess whether this indicates susceptibility to HBV infection and the need and age for booster doses.INTRODUÇÃO: A vacinação é o principal instrumento para prevenir a infecção pelo vírus da hepatite B. Todavia, após a conclusão da série de vacinação, as concentrações de anti-HBs podem diminuir ao longo dos anos e atingir níveis inferiores a 10mUI/mL. A persistência da proteção nestes indivíduos ainda é desconhecida. O presente estudo objetivou determinar os níveis do anticorpo anti-HBs em crianças e adolescentes que receberam o esquema completo de vacinação para a hepatite B. MÉTODOS: O anticorpo para o antígeno de superfície do vírus da hepatite B (anti-HBs foi testado em 371 indivíduos com idade entre 10-15 anos. RESULTADOS: Os voluntários que apresentaram quantidades indetectáveis de anti-HBs corresponderam a 10,2% da população estudada, e 39,9% apresentaram títulos do anticorpo inferiores a 10mUI/mL. Anti-HBs > 10mUI/mL foi verificado em 49,9%. CONCLUS

  7. Presence of anti-HBc is associated to high rates of HBV resolved infection and low threshold for Occult HBV Infection in HIV patients with negative HBsAg in Chile.

    Science.gov (United States)

    Vargas, Jose Ignacio; Jensen, Daniela; Sarmiento, Valeska; Peirano, Felipe; Acuña, Pedro; Fuster, Felipe; Soto, Sabrina; Ahumada, Rodrigo; Huilcaman, Marco; Bruna, Mario; Jensen, Werner; Fuster, Francisco

    2016-04-01

    HBV-HIV coinfection is prevalent. Frequently, anti-HBc is the only serological marker of HBV, which can be indicative of HBV resolved infection, when found together with anti-HBs reactivity; or present as "isolated anti-HBc," related to HBV occult infection with presence of detectable DNA HBV, more prevalent in HIV-positive individuals. Regional data about this condition are scarce. Anti-HBc rapid test has been used as screening, but its performance has not been described in HIV-positive patients. The aim of this study was determine prevalence of anti-HBc in HIV-positive patients, serological pattern of HBV resolved infection and isolated anti-HBc, evaluating presence of HBV occult infection. Assess anti-HBc rapid test compared to ECLIA. Methods included measurement of anti-HBc and anti-HBs in HIV-positive patients with negative HBsAg. Serum HBV DNA quantification and HBV booster vaccination to "isolated anti-HBc" individuals. Detection of anti-HBc by rapid test and ECLIA. In 192 patients, prevalence of anti-HBc was 42.7% (82/192); associated to male gender, drug use, men-sex-men, positive-VDRL, and longer time HIV diagnosis. 34.4% (66/192) had presence of anti-HBs, mean titers of 637 ui/ml. Isolated anti-HBc in 8.3% (16/192), associated to detectable HIV viral load and no-use of HAART; in them, HBV DNA was undetectable, and 60% responded to HBV vaccination booster. Anti-HBc rapid test showed low sensibility (32.9%) compared to ECLIA. These results show that prevalence of anti-HBc in HIV-positive individuals is high, in most cases accompanied with anti-HBs as HBV resolved infection. Low prevalence of "isolated anti-HBc," with undetectable HBV DNA, and most had anamnestic response to HBV vaccination; suggest low possibility of occult HBV infection. Anti-HBc rapid test cannot be recommended as screening method for anti-HBc.

  8. The Expression of Humanized Fab Fragment of the Anti-HBsAg Antibody in Methylotropic Yeast Pichia pastoris%人源性抗HBsAg抗体Fab段在酵母中的表达

    Institute of Scientific and Technical Information of China (English)

    邓宁; 粟宽源; 王珣章; 龙綮新; 杨林; 余宙耀

    2002-01-01

    通过分步整合的方式,将人源性抗乙肝表面抗原(HBsAg)抗体Fab的轻、重链基因分步整合到巴斯德毕赤(Pichia pastoris)酵母GS115菌株的染色体上,经甲醇诱导,成功地分泌表达出抗HBsAg抗体的Fab片段,表达量达50~80mg/L.ELISA结果显示重组酵母分泌表达出的Fab具有较强的结合HBsAg的能力.通过抗Fab的抗体柱亲和层析,纯化出了纯度较高的Fab产品.

  9. 乙型肝炎病毒表面抗体两种检测方法的一致性研究%Study on conformance between two kinds of detection method of anti-HBs antibody

    Institute of Scientific and Technical Information of China (English)

    安哲; 张妮; 李思鹏; 王香玲; 李妙羡

    2014-01-01

    目的:研究外周血乙肝病毒表面抗体定量和定性检测结果之间的一致性。方法采用微粒子酶免疫分析法定量检测,酶联免疫吸附法定性检测。结果以 CMIA 为参考实验,ELISA 法检测 anti-HBs 的 Se 、Sp 、J 、PV +、PV -分别为0.95、0.53、0.48、0.74、0.88,k =0.51;吸光度为0.4009时 Se 、Sp 、J 、PV +、PV -分别为0.50、0.95、0.45、0.93、0.43;对吸光度0.1043~0.4009范围外样本分析,ELISA 定性检测的 Se 、Sp 、J 、PV+、PV-分别为0.90、0.91、0.81、0.93、0.88,k =0.81。结论吸光度值0.105为 ELISA 检测结果判定的 Cut-off 值具有良好的检测灵敏度(Se =0.95)和较好的阴性预测值(PV-=0.88),ELISA 检测 Anti-HBs 阴性可认为 Anti-HBs 浓度小于10 mIU/mL 而不具有保护价值;当样本吸光度大于或等于0.4009时可认为 Anti-HBs 浓度大于或等于10 mIU/mL,具有保护意义;ELISA 检测 Anti-HBs 灰区范围主要是吸光度0.105~0.4009,应定量检测以判定 Anti-HBs 真实水平。%Objective To analyze the conformance between the quantitative and qualitative tests of hepatitis B surface antibody (anti-HBs).Methods The chemiluminecence microparticle enzyme immunoassay(CMIA)was adopted to quantitatively detect anti-HBs and the enzyme linked immunosorbent assay(ELISA)was adopted to qualitatively detect anti-HBs.Results With the CMIA as the reference experiment,Se ,Sp ,J ,PV+ and PV-of anti-HBs detected by ELISA were 0.95,0.53,0.48,0.74 and 0.88 respec-tively,k=0.51;when the absorbance was 0.400 9,Se ,Sp ,J ,PV+ and PV-were 0.50,0.95,0.45,0.93 and 0.43 respectively;for the samples exceeding the absorbance range of 0.104 3 -0.400 9,Se ,Sp ,J ,PV+ and PV-qualitatively detected by ELISA were 0.90,0.91,0.81,0.93 and 0.88 respectively,k =0.81.Conclusion Determining cutoff value with the absorbance value 0.105 as the ELISA detection result has the good detection sensitivity(Se =0.95)and

  10. 重组人抗HBsAg Fab抗体的纯化方法比较研究%Study on the purification of recombinant human anti-HBsAg Fab fragment

    Institute of Scientific and Technical Information of China (English)

    饶桂荣; 粟宽源; 陈文吟; 余宙耀

    2005-01-01

    目的研究重组人抗HBsAg Fab抗体的纯化条件.方法采用羊抗人Fab抗体亲和层析柱、14F7单克隆抗体亲和层析柱、离子交换-分子筛层析柱,分别纯化由酵母工程菌(GS115/Fab)发酵的重组人抗HBsAg Fab抗体,并对3种纯化方法所得Fab抗体的纯度、收率、与HBsAg的结合活性进行比较.结果 3种纯化方法中,14F7单克隆抗体柱纯化的Fab抗体的纯度达98%左右,Fab抗体柱纯化的Fab抗体的纯度为95%,但这两种亲和柱的目的蛋白收率都不高,分别为35%、55%.而离子交换柱纯化的Fab抗体的纯度为93.8%,经分子筛柱进一步纯化后,可达98%以上,Fab抗体蛋白收率可达80%以上.经ELISA分析,3种方法纯化的Fab抗体均具有较高的HBsAg抗原结合力和特异性.结论通过对3种纯化方法的比较得出,离子交换-分子筛层析法是重组人抗HBsAg Fab抗体的最佳纯化方法,这为抗HBsAg Fab抗体的产业化生产和临床研究打下良好基础.

  11. 慢性乙型肝炎患者血清表面抗原与抗体同时阳性结果分析%Analysis of coexistence of hepatitis B surface antigen and anti - HBs antibodies in patients with chronic hepatitis B virus infection

    Institute of Scientific and Technical Information of China (English)

    陈天宝; 范公忍; 李娟; 胡学玲

    2011-01-01

    Objective:To study the relationship between concurrence mode of hepatitis B surface antigen and anti - HBs antibodies in patients with chronic hepatitis B virus infection and HBV DNA. Methods :Serological diagnosis for HBV infection was detected by VITROS Eci/ECiQ immunodiagnostic systems and HBV DNA viral load was quantified by using the commercially a-vailable real -time fluorescence quantitative kit ( PG Biotech). Results:Among the 5717 patients monitored at our hospital, 248 (4.34% ) have been identified as carrying both HBsAg and anti - HBs antibodies. In which, the majority appeared with HBV DNA at the same time. Conclusion: Coexistence of HBsAg and anti - HBs is not rare. HBV DNA concentration is an important parameter to diagnose and treat patients with simultaneous positive for HBsAg and anti - HBs antibodies.%目的:探讨乙型肝炎患者HBsAg和抗HBs共存模式及与HBV DNA的关系.方法:采用增强化学发光法检测血清乙肝标志物,并采用荧光定量PCR法检测HBV DNA.结果:5717例慢性乙肝患者检测出HBsAg和抗HBs双阳性248例,占4.34%,其中多数与HBV DNA同时出现.结论:HBsAg和抗HBs同时阳性并不少见,慢性乙肝患者出现抗HBs,不完全代表病毒复制终止,需结合HBV DNA定量综合分析.

  12. The anti-HBsAg (human type B hepatitis, surface antigen) and anti-HBeAg (human type B hepatitis, e antigen) C18 dibenzocyclooctadiene lignans from Kadsura matsudai and Schizandra arisanensis.

    Science.gov (United States)

    Wu, Ming-Der; Huang, Ray-Ling; Kuo, Li-Ming Yang; Hung, Chia-Cheng; Ong, Chi-Wi; Kuo, Yao-Haur

    2003-11-01

    The C(18) dibenzocyclooctadiene lignans including three novel schizanrin F (1), G (2), H (3), along with the known kadsurarin (4), were isolated from Kadsura matsudai. A new C(19) homolignan named schiarisanrin E (5), together with the known C(18) lignans, gomisin B (6), G (7) and (+)-gomisin K(3) (8) were obtained from Schizandra arisanensis. Gomisin B, G and (+)-gomisin K(3) showed moderate to strong activity for antihepatitis in anti-HBsAg (human type B hepatitis, surface antigen) and/or anti-HBeAg (human type B hepatitis, e antigen) tests. The structural elucidations of new compounds 1-3 and 5 were based on two-dimensional (2D) NMR techniques including COSY, HMQC, HMBC, NOESY and CD spectra. Preliminary structure-activity relationship studies for these isolated lignans are also discussed.

  13. Determinación de las condiciones de ensayo óptimas en un ELISA para la detección de anticuerpos séricos IgG anti-LPS de Pseudomonas aeruginosa O11

    Directory of Open Access Journals (Sweden)

    Tania Valmaseda

    2004-12-01

    Full Text Available La selección de sueros de donantes sanos para la obtención de una gamma hiperinmune contra la infección por Pseudomona aeruginosa, así como la evaluación de la respuesta inmunológica de cualquier candidato vacunal contra este microorganismo necesita contar en el laboratorio con técnicas estandarizadas. En este trabajo se realizaron los ensayos necesarios para el montaje y la optimización de un ELISA indirecto para la determinación de anticuerpos séricos de clase IgG anti- LPS de P. aeruginosa O11. Se evaluaron la concentración de recubrimiento, condiciones de bloqueo, dilución de trabajo de las muestras a evaluar y del conjugado con el fin de seleccionar en cada caso las mejores respuestas para el control positivo, el control negativo y el blanco del ensayo. Una concentración de 1,5 μg/mL de LPS O11 en PBS toda la noche a 4 °C como recubrimiento, la necesidad de no incluir un paso adicional de bloqueo y el conjugado humano anti IgG-HRP diluido 1:3000, 1 h a 37 °C resultaron las variables óptimas para el ensayo. Por otra parte, se estableció el rango lineal de la curva del control positivo y se seleccionó la dilución de trabajo 1:100 para las muestras de sueros a evaluar.

  14. Anti-HBe阳性慢性乙型肝炎患者与Anti-HBs阳性者PD-1及其配体的变化与意义%Clinical significance of peripheral blood PD-1 and its ligands in patients with anti-HBe positive CHB =or anti-HBs positive of normal ALT

    Institute of Scientific and Technical Information of China (English)

    骆华; 杨建杰; 骆方军

    2012-01-01

    目的 分析丙氨酸氨基转移酶(ALT)正常的血清Anti-HBe阳性的慢性乙型肝炎(CHB)患者与Anti-HBs阳性者外周血程序性死亡分子-1(PD-1,CD279)及其配体(CD274和CD273)的变化并探讨其临床意义.方法 分别选择23例、22例和25例ALT正常的血清Anti-HBe阳性的CHB患者、Anti-HBs阳性者和对照者.采用流式细胞术检测其外周血淋巴细胞CD279、CD274和CD273的表达水平.用多元方差分析比较各组的差异.结果 CD279在对照组、Anti-HBe阳性组和Anti-HBs阳性组的表达水平分别为:(33.3±5.4)%、(48.0±9.1)%、(42.8±9.0)%.CD274在对照组、Anti-HBe阳性组和Anti-HBs阳性组的表达水平分别为:(59.4±4.4)%、(71.3±10.3)%、(62.9±10.2)%.CD273在对照组、Anti-HBe阳性组和Anti-HBs阳性组的表达水平分别为:(5.3±2.2)%、(15.6±5.3)%、(5.1±1.0)%.从Anti-HBe阳性组到Anti-HBs阳性组再到正常对照组,CD279和CD274表达水平呈逐渐下降趋势.3个因变量在总体差异有统计学意义(Pillai迹=0.988,F=3090.105,P=0.000),η偏平方为0.458.Anti-HBs阳性组的CD273表达水平与对照组比较,其差异无统计学意义(P=0.082);其他组两两比较,差异均有统计学意义(P<0.01).结论 对于ALT正常的Anti-HBe阳性的CHB患者与Anti-HBs阳性者进行免疫状况的评估是必要的.监测HBeAg血清学转换的CHB患者或Anti-HBs阳性者外周血PD-1及其配体的变化,可更好地了解HBV感染后的病程变化,从而改善HBV感染者的预后.%Objective To study the clinical significance of peripheral blood PD-1 (CD279) and its ligands(CD274 and CD273)between patients with anti-HBe positive chronic hepatitis B (CHB) or anti-HBs positive group of normal glutamic acid alanine aminotransferase (ALT).Methods A total of 70 subjects of normal ALT,including 23 patients with anti-HBe positive,22 anti-HBs positive and 25 normal controls,were enrolled.The expression level of CD279,CD274 and CD273 in peripheral

  15. Level of Anti-HBs Among Children Aged Between 1 and 15 Years in Zhuzhou%株洲市1~15岁儿童乙型病毒性肝炎抗体水平

    Institute of Scientific and Technical Information of China (English)

    唐雨新; 龙术国; 任重

    2011-01-01

    目的 了解株洲市1~15岁儿童抗-HBs水平,评价儿童乙肝疫苗预防接种效果.方法 用单纯随机抽样方法 抽取1~15岁儿童288人,调查既往乙肝疫苗免疫史,每人抽静脉血检测乙肝抗-HBs.分析各年龄段乙肝免疫史与抗体滴度均数、抗-HBs阳性率的关系.结果 288名1~15岁儿童乙肝抗-HBs阳性率为74.31%,有免疫史人群抗-HBs阳性率明显高于无免疫史人群(P<0.01);1~岁、6~15岁年龄组抗-HBs阳性率差异有统计学意义(P<0.05),抗体滴度均数差异无统计学意义(P>0.05);1~15岁儿童乙肝全程接种率为87.15%,其中1~岁为87.22%,6~15岁为87.04%.结论 株洲市1~15岁儿童乙肝全程接种率高,儿童乙肝保护性抗体(抗-HBs)亦处于较高水平,但应在进一步提高乙肝疫苗接种率和首针及时接种率同时,探索适合低/无应答人群的免疫策略,以提高人群免疫水平.%Objective To investigate the level of anti - HBs in children aged between 1 and 5 years, and to evaluate the effect of HBV vaccine in these children. Methods With the simple random sampling method, 228 children aged 1~ 15 years were selected to survey their histories of hepatitis B vaccine inoculation. Their venous blood samples were collected to test anti - HBs antibodies. The correlation between the histories of hepatitis B vaccine inoculation and the average antibody titer, the anti - HBs positive rate in different age- groups was analyzed. Results Among the 288 children, the prevalence rate of HBsAb was 74.31%. The anti - HBs positive rate of children with histories of hepatitis B vaccine inoculation was significantly higher than that of children without the histories (P<0.01). There were statistically significant differences in the anti - HBs positive rate between the 1~ age group and the 6~15 age group (P<0.05), but no statistically significant difference was found in the average antibody titer between the two groups (P>0.05). The

  16. THE DETECTION OF ANTI-HBs,ANTI-HBc,AND HBV-DNA OF 2 274 BLOOD DONORS WITH HBsAg NEGATIVE%2 274名HBsAg阴性献血员抗-HBs、抗-HBc、HBV-DNA检测结果分析

    Institute of Scientific and Technical Information of China (English)

    许顺姬

    2007-01-01

    [目的]检测2 274名HBsAg阴性献血员血清抗-HBs、抗-HBc、HBV-DNA,探讨献血员筛查中检测抗-HBs、抗-HBc的必要性.[方法]对2 274名HBsAg阴性献血员血清ELISA法检测抗-HBs、抗-HBc,用PCR法检测HBV-DNA.[结果]检测者2 274名中①抗-HBs阳性/抗-HBc阴性59.3%,HBV-DNA阳性率0.2%;②抗-HBs阴性/抗-HBc阴性17.4%,HBV-DNA阳性率0.3%;③抗-HBs阴性/抗-HBc阳性1.4%,HBV-DNA阳性率6.3%:④抗HBs阳性/抗-HBc阳性21.9%,HBV-DNA阳性率0.2%.[结论]抗-HBs阴性/抗-HBc阳性群体中HBV-DNA阳性率最高(6.3%),提示献血员筛查中检测抗-HBs、抗-HBc,可以降低通过输血途径的感染率.

  17. Expression of human anti-HBsAg single chain Fab gene in Pichia pastoris%人源性抗HBsAg单链Fab基因在Pichia pastoris中的表达

    Institute of Scientific and Technical Information of China (English)

    邓宁; 向军俭; 粟宽源; 王宏; 唐勇

    2006-01-01

    目的:研究重组Fab的结构与亲和活性的关系.方法:通过重叠PCR,将人源性抗HBsAg Fab的H和L链基因融合构建单链Fab基因,并将其转入毕赤酵母表达载体pPICZαA中.以单链Fab基因表达载体通过氯化锂转化法转化毕赤酵母GS115.将获得的重组酵母在摇瓶中培养进行重组单链Fab的可溶性表达.表达上清经硫酸铵沉淀及亲和层析纯化后,用直接ELISA检测表达产物和纯化Fab的活性.结果:SDS-PAGE和Western blot分析显示,单链Fab在毕赤酵母中获得分泌型表达.薄层扫描显示,在摇瓶中培养毕赤酵母表达的单链Fab约为5~10 mg/L.经亲和层析纯化获得纯度达97.8%的重组单链Fab.经直接ELISA测定的结果显示,重组单链Fab具有较好的结合HBsAg的活性.结论:通过重叠PCR构建的融合单链Fab基因,可成功地在毕赤酵母中获得分泌型表达,表达产物具有较好的结合HBsAg的活性.

  18. Research on time resolved fluorescence immunoassay in quantitative detection of the anti-HBs of children's serum%时间分辨技术定量检测儿童乙肝病毒抗-HBs水平的研究

    Institute of Scientific and Technical Information of China (English)

    程邦宁; 李林; 金载璇; 郝家砚; 孙亮; 许莉莉; 张功

    2011-01-01

    Objective To detect the anti-HBs of children's serum quantitatively, so as to analyze their immunity to hepatitis B virus (HBV). Methods Time resolved fluorescence immunoassay was used to measure the anti-HBs of 3480 serum samples from the children of non-infective HBV. Results There were 817 cases in 0~ mIU/ml about con-tent of the anti-HBs (23.5%), 1142 cases in 10-100 mlU/ml (32.8%), 1 521 cases > 100 mlU/ml (43.7%). There was no statistical difference between deferent sex of the anti-HBs (Z = 0.34,P = 0.735). There was statistical dif-ference between different age groups (Z = 3 634.00, P100 mIU/ml 1 521人(43.7%),不同性别间抗-HBs含量差异无统计学意义(Z=0.34,P=0.735);不同年龄组之间抗-HBs含量差异有统计学意义(Z=3 634.00,P<0.001),且年龄组与抗-HBs含量呈现负相关(rs=-0.217,P<0.001).结论 安徽省部分儿童对HBV的免疫力不容乐观,且随着年龄的增长,抗-HBs水平呈下降趋势,因此定期定量检测抗-HBs含量和加强乙肝疫苗预防接种非常必要.

  19. 重组人源性抗HBsAg Fab抗体纯化方法的比较研究%Study on the Methods of Purification of Recombinant Humanized anti-HBsAg Fab Antibody

    Institute of Scientific and Technical Information of China (English)

    邓宁; 向军俭; 饶桂; 陈文吟

    2004-01-01

    HBsAg Fab抗体被认为在预防和治疗HBV引起的肝病中具有重要的作用.为了建立稳定的、适于生产应用的重组人源性抗HBsAg Fab抗体的纯化工艺,本实验对不同纯化方法进行了比较研究.比较了抗Fab抗体亲和层析、ScFv单克隆抗体亲和层析和离子交换层析等3套纯化工艺在酵母发酵生产的重组人源性抗HBsAg Fab抗体纯化中的效率.结果显示,抗Fab抗体亲和层析柱纯化酵母表达的重组Fab,纯度为96.8%,但回收率偏低,只有30%~40%.ScFv单克隆抗体亲和层析纯化的重组Fab,纯度为97.5%,回收率达75%~85%.该工艺能很好的适应较小规模的生产应用.离子交换层析纯化的重组Fab,纯度为97%,回收率为75%~85%.该工艺能很好的适应较大规模的生产应用.以上结果表明,应用ScFv单克隆抗体亲和层析和离子交换层析纯化技术均能很好的纯化出重组人源性抗HBsAg Fab抗体,这两种纯化工艺不仅大大节约纯化成本,且纯化效率和回收率有很大提高.为重组Fab抗体的工业化生产应用奠定了基础.

  20. Trasplante renal en pacientes con anticuerpos dirigidos contra donante

    Directory of Open Access Journals (Sweden)

    Federico Cicora

    2014-10-01

    Full Text Available En trasplante renal, los anticuerpos donante-específicos por ensayos de fase sólida predicen el rechazo temprano mediado por anticuerpos, incluso con resultados negativos de citometría de flujo o citotoxicidad dependiente del complemento. Aquí se describen los protocolos de inmunosupresión y los resultados a diez meses de cuatro pacientes en los que se detectó anticuerpos donante-específicos anti-antígenos leucocitarios humanos (HLA por Luminex®, pero no detectados por el método de citotoxicidad dependiente de complemento (CDC ni por citometría de flujo. Los cuatro pacientes recibieron tratamiento de inducción con 5 dosis de timoglobulina de 1.25 mg/kg y 5 dosis de inmunoglobulina intravenosa (IVIG de 400 mg/kg. Además, uno recibió 20 mg de basiliximab el mismo día del trasplante y el día 4 postrasplante; otro recibió 3 sesiones de plasmaféresis en los días -5, -3, y -1 y eculizumab en dosis de 1200 mg antes del trasplante, 900 mg el día 1, and 600 mg por semana durante un mes. En todos los casos, la inmunosupresión de mantenimiento consistió en tacrolimus, micofenolato y deltisona. Todos presentaron buenos resultados en el corto plazo. Nuestra experiencia sugiere que los pacientes con anticuerpos donante-específicos anti-HLA detectados solo por Luminex® deben recibir un seguimiento estricto y que en esta población se pueden obtener buenos resultados a partir del uso de terapia de inducción con timoglobulina e IVIG.

  1. Rabbit IgG antibodies against Phospholipase A2 from Crotalus durissus terrificus neutralize the lethal activity of the venom Los anticuerpos IgG de conejos anti-fosfolipasa A2 de Crotalus durissus terrificus neutralizan la actividad letal del veneno

    Directory of Open Access Journals (Sweden)

    Juan P. Rodríguez

    2006-12-01

    Full Text Available Crotalus durissus terrificus (C.d.t. (South American rattlesnake venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2 and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutralize the lethality of the whole venom. PLA2 was isolated by gel filtration chromatography (Sephadex G-75. Specific antibodies were obtained by subcutaneous and intramuscular inoculation of PLA2 (700 µg with Freund adjuvant. Groups of six mice (20 + 2 g were inoculated with 0.5 ml i.p. of C. d. t. venom (4 µg or a mixture of venom that had been preincubated with the desired volume of IgG antibodies. Mortality, recorded 24 and 48 h after inoculation, showed that IgG anti-PLA2 were more effective than anticrotalic serum in neutralizing the lethal activity. These results demonstrate that it could be possible to obtain an anti-venom made by specific antibodies with a high level of protection against the lethal component of C.d.t. venom, and/or the inclusion of these antibodies as a supplement in heterologous anti-venoms.El veneno de Crotalus durissus terrificus (C.d.t. (Cascabel de Sud América posee actividad miotóxica y neurotóxica, actividades que también exhibe el complejo crotoxina, principal componente tóxico de este veneno. El complejo crotoxina está constituido por una fosfolipasa A2 básica (PLA2 y una proteína acídica no tóxica, el crotapotín. En este trabajo se estudió la capacidad neutralizante de anticuerpos IgG anti-PLA2 sobre la letalidad inducida por el veneno entero. El antígeno PLA2, fue aislado por cromatografía de filtración en gel (Sephadex G-75. Se inocularon conejos machos por vía subcutánea e intramuscular, con 700 µg de PLA2 y adyuvante para la obtención de anticuerpos específicos. La capacidad neutralizante del

  2. 郑州市某民办学校学生HBsAg阳性率和抗-HBs水平分析%Analysis on the positive rate of HBsAg and anti-HBs level among students of a voluntary school in Zhengzhou City

    Institute of Scientific and Technical Information of China (English)

    苗秋丽; 臧改华; 司珩

    2006-01-01

    目的了解近年来乙肝疫苗普遍接种后,郑州市某民办学校学生的HBsAg阳性率和抗-HBs水平状况.方法对2003~2004年郑州市民办某小哈佛双语学校465名5~15岁入学新生进行了血清免疫检测(HBsAg和抗-HBs).结果HBsAg阳性率为0.4%(2/465),较1992年有大幅度下降,2003~2004年0~6岁年龄组未检出;抗-HBs阳性率为58.5%(272/465),较1992年河南省调查的抗-HBs阳性水平有大幅度提高.结论抗-HBs阳性率随年龄增大而降低,而HBsAg阳性率则随年龄增大而升高,提示加强接种疫苗的重要性.

  3. Anticuerpo anticitrulina y manifestaciones extra articulares en artritis reumatoidea

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    María Jezabel Haye Salinas

    2013-02-01

    Full Text Available Los pacientes con artritis reumatidea (AR pueden desarrollar manifestaciones extra articulares (MExA, relacionadas a su morbi-mortalidad. Los anticuerpos anti-péptidos citrulinados cíclicos (ACCP son específicos para la AR y estan relacionados con el daño articular; y podrían tener rol patogénico en las MExA. Nuestro objetivo fue determinar la relación entre los anticuerpos ACCP y MExA en pacientes con AR. Se incluyeron 74 pacientes con diagnóstico de AR (ACR 1987 mayores de 18 años, de más de 6 meses de evolución, con MExA, y un control apareado por sexo y edad sin MExA por cada paciente. Las variables demográficas, clínicas y de laboratorio se compararon con test t, chi cuadrado o Mann-Whitney. Se realizó análisis multivariado; p ≤ 0.05. Los pacientes con MExA presentaron mayor título de anticuerpo ACCP (116 vs. 34, p < 0.01 y de factor reumatoideo (FR (108 vs. 34.5, p < 0.01. En el análisis multivariado hubo asociación entre la presencia de MExA y tabaquismo activo (p = 0.02, OR: 3.78, IC 95%: 1.17-12.2, FR positivo (p = 0.04, OR: 3.23, IC95%: 1.04-11.8 y anticuerpo ACCP positivo (p = 0.04, OR: 3.23, IC 95%: 1.04-10. Presentaron mayor título de anticuerpo ACCP que los controles los pacientes con xerostomía (109 vs. 34, p = 0.04, xeroftalmia (150 vs. 34, p < 0.01, nódulos sub-cutáneos (NSC (141 vs. 34, p < 0.01 y fibrosis pulmonar (158 vs. 34, p = 0.04. En conclusión, el anticuerpo ACCP positivo, el FR positivo y el tabaquismo activo fueron factores de riesgo independientes para el desarrollo de MExA.

  4. 国产抗-HBs定性试剂评估人群对乙型肝炎病毒的免疫状态%Evaluation of domestic qualitative reagents for detecting anti-HBs in assessing the immunity to hepatitis B virus

    Institute of Scientific and Technical Information of China (English)

    冯振华; 毕永春; 张姝; 陈洁; 胡娅莉; 周乙华

    2013-01-01

    目的:探讨国产抗-HBs定性试剂在评估人群对乙型肝炎病毒(HBV)有无免疫力的应用价值.方法:对1016例HBsAg阴性血清,分别用国产定性试剂和美国雅培定量试剂检测抗-HBs,比较结果的一致性和差异.结果:639例(62.9%)血清国产试剂检测抗-HBs阳性,742例(73.0%)雅培试剂检测阳性(≥10 mIU/ml,x2=23.98,P<0.001).在抗-HBs≥100 mIU/ml的444例、50 mIU/ml ~99 mIU/ml的83例和10 mIU/ml~49 mIU/ml的215例,国产试剂检测阳性率分别为99.1%、92.8%和52.1%.在雅培试剂检测抗-HBs<10 mIU/ml的274例中,国产试剂98.2%阴性.结论:国产抗-HBs定性试剂具有良好的特异性,虽然对低水平抗-HBs阳性血清的检出率较低,但抗-HBs ≥50 mIU/ml时,具有良好的检出率.国产定性试剂抗-HBs阳性,说明对HBV有免疫力,通常无需定量检测.%Objective:To assess the value of domestic qualitative reagents for anti-HBs in evaluating immunity to hepatitis B virus (HBV).Methods:A total of 1016 sera with negative HBsAg were tested for anti-HBs with domestic qualitative ELISA and Abbott quantitative reagents.The agreement and differences of the results with various reagents were statistically analyzed.Results:Of the 1016 sera,639 cases (62.9%) and 742 cases(73.0%) were positive for anti-HBs detected by the domestic reagents and Abbott quantitative reagents respectively (x2 =23.98,P <0.001).By detection with the domestic reagents,four hundred and forty (99.1%) of 444 sera with anti-HBs ≥100 mIU/ml and 77 (92.8%) of 83 sera with anti-HBs 50-99 mIU/ml were also positive.However,domestic qualitative reagents showed positive only in 112 (52.1%) of 215 sera with anti-HBs 10-49 mIU/ml.On the other hand,269 (98.2%) of 274 sera with anti-HBs < 10 mIU/ml were also negative detected with the domestic reagents.Conclusion:The domestic qualitative reagents for anti-HBs show high specificity and high agreement with Abbott quantitative reagents in

  5. In vivo activity of a mixture of two human monoclonal antibodies (anti-HBs) in a chronic hepatitis B virus carrier chimpanzee

    NARCIS (Netherlands)

    R. Heijtink; W. Paulij; P.A.C. van Bergen (Patrick); M.H. van Roosmalen (Mark); D. Rohm; B. Eichentopf; E. Muchmore; A.D.M.E. Osterhaus (Albert); R.A. de Man (Robert)

    1999-01-01

    textabstractA 35-year-old female hepatitis B virus carrier chimpanzee was infused with one dose of a mixture of human monoclonal antibodies 9H9 and 4-7B (antibodies against hepatitis B virus surface antigen; HBsAg). Blood samples were taken before and up to 3 weeks afte

  6. The Analysis of 22 Cases of Occult Hepatitis B with Positive Anti-HBs%表面抗体阳性隐匿性乙型肝炎22例分析

    Institute of Scientific and Technical Information of China (English)

    孙和才; 李淑丽

    2013-01-01

    目的:了解乙型肝炎表面抗体(抗-HBs)阳性隐匿性乙型肝炎的发病情况、临床特点及可能的发病机制。方法对158例抗-HBs阳性者进行血清HBVDNA荧光定量筛查,对检测出HBV DNA的22例乙型肝炎表面抗体(抗-HBs)阳性隐匿性乙型肝炎患者进行临床、生化、影像、组织病理及免疫组化检查分析。结果22例乙型肝炎表面抗体(抗-HBs)阳性隐匿性乙型肝炎患者中部分患者有慢性肝病的特征,影像学检查13.64%有终末期肝病表现;有不同程度肝功能异常者达68.18%;HBV DNA大多(86.36%)在1×102~103IU/mL之间;对影像学检查未见异常的病例肝组织穿刺活检63%有炎症活动,有纤维化表现者达81.8%。结论隐匿性乙型肝炎是HBV感染的特殊形式。表面抗体(抗-HBs)阳性隐匿性乙型肝炎,是终末期隐匿性肝脏疾病的原因之一。抗-HBs与HBV DNA同时存在是由HBV变异等多因素的结果,应引起关注。%Objective To find out the prevalence, clinical manifestations and possible mechanisms of occult hepatitis B with positive anti-HBs. Methods Screen serum HBV-DNA in 158 patients with positive anti-HBs by qRT-PCR and analyze the clinical, biochemical, imaging, histopathological and immunohistochemical data in patients of occult hepatitis B with positive anti-HBs. Results 22 cases of occult hepatitis B with positive anti-HBs were found out, part of them showing chronic hepatitis. End-stage liver disease was highly doubted in 13.64%patients through imaging studies. Abnormal liver function with different stages appeared in 68.18%patients by biochemical tests. The concentration of HBV-DNA in 86.36%patients fluctuated between 1×102~1 ×103IU/mL. In patients with normal imaging studies, inflammation was found out in 63%and fibrosis in 81.8%through immunohistochemical analysis. Conclusion Occult hepatitis B is a special form of HBV infection, occult hepatitis B with positive anti-HBs

  7. 乙型肝炎病毒表面抗原和表面抗体同时阳性样本的体外中和反应特性分析%Study on neutralization reaction characteristics in vitro of HBsAg and anti-HBs coexist positive samples

    Institute of Scientific and Technical Information of China (English)

    朱浩稳; 黄剑臻; 林曼跃

    2012-01-01

    目的 研究同时阳性样本的乙型肝炎病毒(HBV)表面抗原(HBsAg)和乙型肝炎病毒表面抗体(抗-HBs)的体外中和反应特性.方法 运用化学发光定量检测技术在HBsAg阳性样本中收集抗-HBs同时阳性的样本血清作为研究对象;选择单纯HBsAg阳性的样本和单纯抗-HBs阳性的样本分别与同时阳性的研究组样本中的抗-HBs和HBsAg进行体外血清学中和反应,测定中和物中的HBsAg和抗-HBs含量;测定HBsAg单阳性样本的基因型和HBsAg亚型,记录各测定结果.结果 获取1例HBsAg(130.21 ng/mL)和抗-HBs(50.6 U/L)双阳性样本,HBV基因型为B型,HBsAg亚型为adw;20例HBsAg单阳性样本中有6例能中和研究组样本中的抗-HBs(中和物抗- HBs≤6.5 U/L);20例抗-HBs单阳性样本均能中和研究组样本中的HBsAg(中和物HBsAg≤0.06ng/mL).结论 同时阳性样本中的抗-HBs不是与其共存的HBsAg的特异性抗体.

  8. Auto-anticorpos anti-β2-glicoproteína I e síndrome metabólica Anticuerpos anti-β2-glicoproteína I y síndrome metabólico Anti-beta2-glycoprotein I autoantibodies and metabolic syndrome

    Directory of Open Access Journals (Sweden)

    Rodrigo B. Krás Borges

    2011-04-01

    Full Text Available FUNDAMENTO: A síndrome metabólica (SM é uma entidade pró-aterogênica. Autoanticorpos tais como β2-glicoproteína I (β2-gpI podem influenciar o aparecimento de ateromas. Estudos anteriores confirmaram uma associação entre anticorpos IgA anti-β2-gpI e isquemia cerebral, infarto do miocárdio, doença arterial periférica e doença da carótida. OBJETIVO: O objetivo desse estudo de caso-controle foi avaliar uma possível associação entre anticorpos anti-β2-gpI e anticardiolipina (aCL com SM não-complicada. MÉTODOS: Pacientes com SM sem histórico de eventos vasculares e indivíduos-controle, consistindo em pacientes da Enfermaria de Ortopedia admitidos devido a doenças musculoesqueléticas foram incluídos no estudo. Idade, sexo, etnia, histórico de hipertensão, tabagismo, hipercolesterolemia e diabetes mellitus foram avaliados como fatores de risco em ambos os grupos. Anticorpos IgG, IgM, e IgA anti-β2-gpI e aCL foram detectados através de imunoensaios enzimáticos. RESULTADOS: Um total de 68 pacientes com SM e 82 controles foram estudados. Os pacientes com SM tinham média de idade superior à dos controles (P = 0,001, enquanto homens (P = 0,003; OR 0,31; IC95%: 0,15-0,16 e etnia caucasiana (P = 0,004; OR 0,25; IC95%:0,10-0,60 eram predominantes nos controles. Histórico de hipertensão, hipercolesterolemia e diabetes mellitus foi mais prevalente nos pacientes com SM do que nos controles (P FUNDAMENTO: El síndrome metabólico (SM es una entidad pro-aterogénica. Autoanticuerpos tales como β2-glicoproteína I (β2-GPI pueden influir en la aparición de ateromas. Estudios previos han confirmado una asociación entre anticuerpos IgA anti-β2-GPI y la isquemia cerebral, infarto de miocardio, enfermedad arterial periférica y enfermedad carotidea. OBJETIVO: El objetivo de este estudio de caso-control fue evaluar una posible asociación entre los anticuerpos anti-β2-GPI y anticardiolipina (aCL con SM complicada. MÉTODOS: Se

  9. Neuromielitis óptica con alta expresión de acuaporina-4 y anticuerpos anti-acuaporina-4 positivos en suero Neuromyelitis optica with high aquaporin-4 expression and positive serum aquaporin-4 autoantibodies

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    Alejandra Báez

    2012-04-01

    Full Text Available La presencia de anticuerpos IgG en suero, con blanco en los canales de acuaporina-4, es específica de la neuromielitis óptica (NMO. El 60% de los pacientes con NMO presentan lesiones cerebrales en la resonancia magnética (RM; en un 8% (mayoría niños estas lesiones se consideraron "atípicas". Presentamos dos pacientes con NMO y lesiones en el SNC de alta expresión de acuaporina-4. Caso 1: varón de 50 años, que comenzó con pérdida de visión en ojo derecho (OD. Recibió tratamiento empírico con metilprednisolona 1 g/d x 3 días. Al mes presentó dolor generalizado y hemiparesia derecha; nuevamente recibió metilprednisolona 1 g/d x 5 días e IgG IV 400 mg/kg/d × 5 días. Recuperó la deambulación persistiendo el dolor y fenómenos paroxísticos en los 4 miembros. Potenciales evocados visuales: P100, ojo izquierdo (OI 123 mseg. OD sin respuesta. La RM de cerebro (FLAIR mostró hiperintensidad en nervio óptico derecho, hipotálamo y comisura blanca anterior. RM cervical: lesión medular extensa (5 cuerpos vertebrales. Caso 2: mujer de 53 años, con disminución de la agudeza visual en ambos ojos y parestesias en miembros inferiores que remitieron espontáneamente. Evolucionó al mes con cuadriparesia e incontinencia esfinteriana. Recibió metilprednisolona 1 g/d x 5 días, sin mejoría. Potenciales evocados visuales: P100 OI 124 mseg. OD 128 mseg. RM cerebro: (FLAIR hiperintensidad hipotalámica y periacueductal. RM cervical: lesión medular extensa (7 cuerpos vertebrales. Anticuerpos anti-acuaporina-4 positivos en ambos pacientes (inmunofluorescencia indirecta. Las lesiones consideradas "atípicas", como aquí, en sitios con alta densidad de proteínas canales de agua AQP4 deberán considerarse para el diagnóstico diferencial.Disease-specific aquaporin-4 antibodies (NMO-IgG are the main effector of lesions in neuromyelitis optica (NMO patients. Brain MRI lesions are detected in 60% of them, with 8% (almost infants at sites of high

  10. Immunofluorescence detection of new antigen-antibody system (delta/anti-delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers.

    Science.gov (United States)

    Rizzetto, M; Canese, M G; Aricò, S; Crivelli, O; Trepo, C; Bonino, F; Verme, G

    1977-01-01

    A new antigen-antibody system associated with the hepatitis B virus and immunologically distinct from the HB surface, core, and e systems is reported. The new antigen, termed delta, was detected by direct immunofluorescence only in the liver cell nuclei of patients with HBsAg positive chronic liver disease. At present, the intrahepatic expression of HBcAg and delta antigen appears to be mutually exclusive. No ultrastructural aspect corresponding to the delta antigen could be identified under the electron microscope. delta antibody was found in the serum of chronic HBsAg carriers, with a higher prevalence in patients with liver damage. The nuclear fluorescence patterns of HBcAg and delta antigen were similar; it is only possible to discriminate between the two antigens by using the respective specific antisera. Images Figure PMID:75123

  11. Marcadores inflamatórios e anticorpos anti-chlamydia em pacientes com síndrome metabólica Marcadores inflamatorios y anticuerpos anti-chlamydia en pacientes con síndrome metabólico Inflammatory markers and antichlamydial antibodies in patients with metabolic syndrome

    Directory of Open Access Journals (Sweden)

    Rosecler Riethmuller Franco

    2011-02-01

    ão mostraram diferença significativa em pacientes com SM, com e sem eventos.FUNDAMENTO: El síndrome metabólico está asociado al aumento de riesgo de eventos cardiovasculares. Marcadores inflamatorios y anticuerpos anti-Chlamydia han sido relacionados al desarrollo y a la progresión de la aterosclerosis y de los eventos cardiovasculares. OBJETIVO: Evaluar los marcadores inflamatorios interleucina-6 (IL-6 y factor de necrosis tumoral-alfa (TNF-α y los anticuerpos anti-Chlamydia pneumoniae en pacientes con síndrome metabólico (SM, con y sin eventos cardiovasculares. MÉTODOS: Estudio transversal constituido por 147 individuos. De estos, 100 (68% con SM y sin eventos cardiovasculares; y 47 (32% con SM y con eventos cardiovasculares. De los individuos que habían sufrido eventos cardiovasculares, 13 (6,11% presentan infarto agudo de miocardio (IAM, y diez (4,7%, accidente cerebro vascular (ACV. El diagnóstico del SM fue determinado por los criterios del NCEP-ATPIII. RESULTADOS: La media de edad de los sujetos con eventos cardiovasculares fue de 61,26 ± 8,5 y de 59,32 ± 9,9 en los individuos sin esos eventos (p=0,279, habiendo predominio del sexo femenino. El grupo con SM y sin evento presentó mayor peso, altura, IMC y circunferencia abdominal. Para los individuos con eventos cardiovasculares (p=0,001, los marcadores inflamatorios IL-6 y TNF-α y la enfermedad vascular periférica fueron significativamente mayores. Se obtuvieron niveles elevados de anticuerpos IgG para Chlamydia pneumoniae en el grupo SM, sin eventos y de IgA en el grupo con eventos cuando fueron comparados los dos grupos. Con relación al IAM y al ACV, los anticuerpos anti-Chlamydia pneumoniae no demostraron significación estadística, comparados al grupo sin eventos cardiovasculares. Asociación fue observada con el uso de estatinas, hipoglicemiantes orales, inyectables y antiinflamatorios no esteroides en el grupo con esos eventos. CONCLUSIÓN: Marcadores inflamatorios se encuentran

  12. Application of a newly developed high-sensitivity HBsAg chemiluminescent enzyme immunoassay for hepatitis B patients with HBsAg seroclearance.

    Science.gov (United States)

    Shinkai, Noboru; Matsuura, Kentaro; Sugauchi, Fuminaka; Watanabe, Tsunamasa; Murakami, Shuko; Iio, Etsuko; Ogawa, Shintaro; Nojiri, Shunsuke; Joh, Takashi; Tanaka, Yasuhito

    2013-11-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstrated seroclearance by the Abbott Architect assay. The performance of the Lumipulse HBsAg-HQ assay was compared with that of a quantitative HBsAg detection system (Abbott Architect) and the Roche Cobas TaqMan HBV DNA assay (CTM) (lower limit of detection, 2.1 log copies/ml) using blood serum samples from patients who were determined to be HBsAg seronegative by the Abbott Architect assay. Ten patients had spontaneous HBsAg loss. Of 8 patients treated with nucleotide analogues (NAs), two were HBsAg seronegative after stopping lamivudine therapy and 6 were HBsAg seronegative during entecavir therapy. Eight acute hepatitis B (AH) patients became HBsAg seronegative. Of the 26 patients, 16 were HBsAg positive by the Lumipulse HBsAg-HQ assay but negative by the Abbott Architect assay. The differences between the two assays in terms of detectable HBsAg persisted over the long term in the spontaneous loss group (median, 10 months), the NA-treated group (2.5 months), and the AH group (0.5 months). In 9 patients, the Lumipulse HBsAg-HQ assay detected HBsAg when HBV DNA was negative by the CTM assay. HBsAg was also detected by the Lumipulse HBsAg-HQ assay in 4 patients with an anti-HBs concentration of >10 mIU/ml, 3 of whom had no HBsAg escape mutations. The automatic, highly sensitive HBsAg CLEIA Lumipulse HBsAg-HQ is a convenient and precise assay for HBV monitoring.

  13. Long-term persistence of T cell memory to HBsAg after hepatitis B vaccination

    Institute of Scientific and Technical Information of China (English)

    Ru-Xiang Wang; Greet J. Boland; Jan van Hattum; Gijsbert C. de Gast

    2004-01-01

    AIM: To determine if the T cell memory to HBsAg can persist for a long time after hepatitis B (HB) vaccination.METHODS: Thirty one vaccine recipients who were healthcare workers (18 females and 13 males aged 34-58 years) from Utrecht University Hospital, Netherlands, and had previously Received a standard course of vaccination for hepatitis B were investigated and another 9 unvaccinated healthy volunteers from the same hospital were used as the control. Blood samples were taken just before the experiment to test serum anti-HBs levels and the subjects were classified into different groups according to their serum titers of anti-HBs and vaccination history. Their peripheral blood mononuclear cells (pBrvMc) were isolated from freshly heparinized venous blood and the proliferative response of Tlymphocytes to the recombinant hepatitis B surface antigen(HBsAg) was investigated.RESULTS: Positive serum anti-HBs was found in 61.3%(19/31) vaccine recipients and a significant in vitro lymphocyte proliferative response to recombinant HBsAg was observed in all the vaccinees with positive anti-HBs. Serum anti-HBs level ≤10 IU/L was found in 38.7% (12/31)subjects. In this study, we specially focused on lymphocyte proliferative response to recombinant HBsAg in those vaccine recipients with serum anti-HBsAg less than 10 IU/L.Most of them had Received a standard course of vaccination about 10 years before. T lymphocyte proliferative response was found positive in 7 of the 12 vaccine recipients. These results confirmed that HBsAg-specific memory T cells remained detectable in the circulation for a long time after vaccination, even when serum anti-HBs level had been undetectable.CONCLUSION: The T cell memory to HBsAg can persist for at least 10 years after HB vaccination. Further booster injection is not necessary in healthy responders to HB vaccine.

  14. Immunization with HBsAg-Fc fusion protein induces a predominant production of Th1 cytokines and reduces HBsAg level in transgenic mice

    Institute of Scientific and Technical Information of China (English)

    MENG Zhe-feng; WANG Hua-jing; YAO Xin; WANG Xuan-yi; WEN Yu-mei; DAI Jian-xin; XIE You-hua; XU Jian-qing

    2012-01-01

    Background The Fc receptor associated pathway might improve the immune responses against hepatitis B virus (HBV) as previously described by us.In addition,the Flt3 ligand (FL) has been reported to potentiate antigen presenting cells in vivo and may act as a potential adjuvant to boost antigen-specific immune responses.In this study,the immune efficacies of a set of fusion proteins of HBsAg and Fc and/or FL were evaluated in HBsAg transgenic mice.Methods The fusion proteins composed of HBsAg and the Fc domain of murine IgG1 (HBsAg-Fc) and/or the Flt3 ligand,and yeast-derived recombinant HBsAg were used as immunogen to immunize HBsAg transgenic mice,respectively.Serum and liver HBsAg levels,serum anti-HBsAg and cytokine profile,and the activities of alanine aminotransferase (ALT)/AST were investigated after immunization.Results After six injections,the most pronounced decrease in serum and liver HBsAg levels was observed in the HBsAg-Fc immunized group.In addition,serum Th1 cytokines and ALT/AST activities were highest in this group,indicating an effective induction of a favorable cellular immune response.Interestingly,the fusion protein containing HBsAg-Fc and the Flt3 ligand stimulated an alternative Th1-type immune response featured with high level productions of tumor necrosis factor α (TNF- α) and monocyte chemoabstractant protein 1 (MCP-1),causing a more severe cytotoxicity in hepatocytes while showed less effective in reducing serum HBsAg level.Conclusion HBsAg-Fc is effective in eliciting both the humoral and cellular immune responses against HBsAg in HBsAg transgenic mice,which makes it a potential immunogen for the immunotherapy of chronic hepatitis B.

  15. Immunogenicity of recombinant HBsAg/HCV particles in mice pre-immunised with hepatitis B virus-specific vaccine.

    Science.gov (United States)

    Netter, Hans J; Woo, Wai-Ping; Tindle, Robert; Macfarlan, Roderick I; Gowans, Eric J

    2003-06-20

    Due to their spatial structure virus-like particles (VLPs) generally induce effective immune responses. VLPs derived from the small envelope protein (HBsAg-S) of hepatitis B virus (HBV) comprise the HBV vaccine. Modified HBsAs-S VLPs, carrying the immunodominant hypervariable region (HVR1) of the hepatitis C virus (HCV) envelope protein E2 within the exposed 'a'-determinant region (HBsAg/HVR1-VLPs), elicited HVR1-specific antibodies in mice. A high percentage of the human population is positive for anti-HBsAg antibodies (anti-HBs), either through vaccination or natural infection. We, therefore, determined if pre-existing anti-HBs could influence immunisation with modified VLPs. Mice were immunised with a commercial HBV vaccine, monitored to ensure an anti-HBs response, then immunised with HBsAg/HVR1-VLPs. The resulting anti-HVR1 antibody titre was similar in mice with or without pre-existing anti-HBs. This suggests that HBsAg/HVR1-VLPs induce a primary immune response to HVR1 in anti-HBs positive mice and, hence, they may be used successfully in individuals already immunised with the HBV vaccine.

  16. Thyroid autoantibodies in autoimmune diseases Anticuerpos antitiroideos en enfermedades autoinmunes

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    Regina M. Innocencio

    2004-06-01

    Full Text Available Abnormalities in the thyroid function and thyroid autoantibodies have been frequently described in patients with autoimmune diseases but seldom in antiphospholipid syndrome patients. In order to determine the prevalence of thyroid function and autoimmune abnormalities, we compared serum thyrotropin (TSH, serum free thyroxine (T4 levels, thyroid antithyroglobulin (TgAb and antithyroperoxidase (TPOAb levels of 25 patients with systemic sclerosis, 25 patients with rheumatoid arthritis and 13 patients with antiphospholipid syndrome to a control group of 113 healthy individuals. Evaluation included a thorough clinical examination with particular attention to thyroid disease and a serologic immune profile including rheumatoid factor, antinuclear and anticardiolipin antibody measurements. Subclinical hypothyroidism (4.2Ciertas anormalidades en la función tiroidea y anticuerpos antitiroideos han sido frecuentemente descriptos en pacientes con enfermedades autoinmunes, y más raramente en pacientes con el síndrome antifosfolipídico. Para determinar la prevalencía de anormalidades en la función tiroidea y de autoinmunidad, comparamos los niveles séricos de tirotropina (TSH tiroxina libre en suero (T4 anticuerpos antitiroglobulina (TgAb y antitiroperoxidasa (TPOAb en 25 pacientes con esclerosis sistémica, 25 pacientes con artritis reumatoidea y 13 pacientes con el síndrome antifosfolipídico con un grupo control de 113 individuos aparentemente sanos. La evaluación incluyó un completo examen clínico con particular atención para las enfermedades de la tiroides y una evaluación inmunológica incluyendo dosaje del factor reumatoideo, anticuerpos antinucleares y anticardiolipina. Hipotiroidismo subclínico (4.2anticuerpos anti-tiroglobulina (TgAb y/o anti-peroxidasa (TPOAb estaban presentes en 21/63 (33% de los

  17. Factors associated with anti-HBs titers in patients with hepatitis B diseases in the early stage following liver transplantation%乙型肝炎患者肝移植后早期抗HBs滴度的影响因素分析

    Institute of Scientific and Technical Information of China (English)

    王凯; 朱志军; 郑虹; 邓永林; 潘澄; 孙丽莹; 沈中阳

    2014-01-01

    Objective To identify the changes of anti-HBs titers of patients with hepatitis B virus (HBV)-related diseases in the early stage (within the first week) post-liver transplantation (LT) and analyze their influencing factors.Method A total of 26 patients were enrolled in this study.They were all positive for HBsAg pre-LT and received the prophylaxis of lamivudine in combination with intravenous hepatitis B immunoglobulin (HBIG) in the first week post-LT.The titers of anti-HBs were detected daily in blood and drainage fluid every day in the first week post-LT.If the anti-HBs titers were greater than 1000 IU/L,blood and drainage were diluted,then detected again.Result The titers of anti-HBs in HBV-DNA negative groups,low HBsAg groups,and HBeAg negative groups were higher than those in the HBV-DNA positive groups,high HBsAg groups and HBeAg positive groups in the first five days post-LT.The median titer of anti-HBs in drainage fluid was 181.60 IU/L (0.00-968.50 IU/L).And the titer of anti-HBs in drainage fluid was correlated with anti-HBs titers in blood at the same time (r =0.927,P =0.000).The amount of anit-HBs calculated in drainage fluid was very high,but it fluctuated in a wide range (0.00-908.55 IU).Conclusion In the early stage post-LT,patients in high risk groups should receive higher doses of HBIG to maintain safe levels of anti-HBs,while the lower doses of HBIG are enough to the patients in low risk groups.Furthermore,the anti-HBs titers in blood aren't affected by the anti-HBs loss in drainage fluid.%目的 分析乙型肝炎相关性终末期肝病患者接受肝移植术后早期(1周内)外周血中抗乙型肝炎病毒(HBV)表面抗体(抗HBs)滴度的变化及其影响因素.方法 纳入26例肝移植受者,术前HBV表面抗原(HBsAg)检测均为阳性,术后1周内均接受口服拉米夫定与静脉注射乙型肝炎免疫球蛋白(HBIG)治疗.术后第1周内每天检测腹腔引流液及外周血中抗HBs滴度.若抗HBs滴度大于1 000 IU/L,

  18. Prevalencia de anticuerpos anti-Chlamydia trachomatis y anti-Neisseria gonorrhoeae en grupos de individuos de la población mexicana Prevalence of antibodies against Chlamydia trachomatis and Neisseria gonorrhoeae in Mexican populations

    Directory of Open Access Journals (Sweden)

    María del Carmen Cravioto

    2003-01-01

    Full Text Available OBJETIVO: Estimar la prevalencia de infección por Chlamydia trachomatis (CT y Neisseria gonorrhoeae (NG en grupos de individuos con diferente riesgo para infecciones de transmisión sexual. MATERIAL Y MÉTODOS: Estudio transversal multicéntrico efectuado en el Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, de la Ciudad de México, de enero de 1992 a diciembre de 1993, en el que se estudiaron 945 individuos en edad reproductiva, 585 mujeres y 360 hombres. Según su riesgo para infecciones de transmisión sexual se clasificaron en grupos de alto y bajo riesgo. Los de alto riesgo incluyeron mujeres infértiles con daño tubario, mujeres con embarazo ectópico o aborto, hombres infértiles, pacientes con VIH/SIDA, hombres homo o bisexuales y trabajadoras del sexo comercial (TSC. Los de bajo riesgo: mujeres primigestas, hombres fértiles y mujeres infértiles sin daño tubario. Se determinó en duplicado IgG e IgA anti-NG y anti-CT en suero, por análisis inmunoenzimático, utilizando como antígeno el pili de NG y la fracción L1 de CT. Se calcularon porcentajes. RESULTADOS: En mujeres la prevalencia para NG fue IgG 13.7% e IgA 14.3%, y para CT fue IgG 11.4% e IgA 4.4%. En hombres, NG 3.3% y 13.3%, respectivamente, y para CT 7.2% y 5.5%, respectivamente. En TSC se encontró NG en 31.2 % y 28.4%, respectivamente, y para CT 25.0% y 5.7%, respectivamente. En mujeres con infertilidad por daño tubario, NG 5.6% y 9.8%, respectivamente, y CT 8.4% y 1.4%, respectivamente. En 110 primigestas jóvenes, NG 4.5% y 10.0%, respectivamente, y CT 3.6% y 9.1%, respectivamente. CONCLUSIONES: Estos datos confirman la prevalencia elevada de Neisseria gonorrhoeae y Chlamydia trachomatis en trabajadoras del sexo comercial y en hombres homo/bisexuales, pero no en otros grupos de alto riesgo como las mujeres infértiles, con aborto o embarazo ectópico.OBJECTIVE: To estimate the prevalence of Chlamydia trachomatis (CT and Neisseria gonorrhoeae

  19. 孕期注射乙肝免疫球蛋白阻断HBV宫内感染及对婴儿产生anti-HBs的影响%Blocking HBV intrauterine infection by injection of hepatitis B immunoglobulin during gestational period and the effect on production of anti-HBs in infants

    Institute of Scientific and Technical Information of China (English)

    李淑红; 贾蓓; 孙红; 董梅; 刘志芬

    2013-01-01

    目的:探讨孕期注射乙肝免疫球蛋白(hepatitis B immunoglobulin,HBIG)对HBV母婴传播的影响及婴儿对乙肝疫苗免疫应答情况.方法:以HBsAg阳性孕妇及其新生儿为研究对象,孕期注射乙肝免疫球蛋白的孕妇300例及新生儿为实验组,未使用者80例为对照组,比较两组新生儿宫内感染率及出生时和7个月抗体产生情况.结果:孕期注射HBIG组与未注射组新生儿宫内感染率分别为5.33% (16/300)和1.25% (1/80),差异无统计学意义(x2=2.464,P>0.05);孕期注射HBIG组与未注射组新生儿出生时anti-HBs阳性率分别为10.33% (31/300)和1.25% (1/80),差异有统计学意义(x2=6.757,P<0.05),7个月龄anti-HBs产生率为96.12% (124/129)和95.35% (41/43),差异无统计学意义(x2=0.049,P>0.05);出生时anti-HBs阳性的新生儿25例,7个月复查均产生anti-HBs,而出生时anti-HBs阴性或弱阳性的147例婴儿,7个月复查有7例婴儿anti-HBs仍为阴性或弱阳性,但差异无统计学意义(x2=0.321,P>0.05).结论:孕期注射HBIG不能降低HBV宫内感染率,可提高新生儿出生时anti-HBs阳性率,但对7个月anti-HBs产生率无影响.%Objective: To explore the effect of injection of hepatitis B immunoglobulin ( HBIG) during gestational period on maternal - infantile transmission of hepatitis B virus (HBV) and immune response of infants to HBV vaccine. Methods: The pregnant women with positive HBsAg and their neonates were selected as research objects, 300 pregnant women who were treated with injection of HBIG during gestational period and their neonates were selected as experimental group, and 80 pregnant women who didnt receive the treatment and their neonates were selected as control group; the incidence rates of intrauterine infection of neonates and the productions of HBV antibodies at seven months after birth were compared between the two groups. Results: The incidence rates of intrauterine infection in experimental group and

  20. Diagnóstico diferencial en la encefalitis por anticuerpos contra el receptor NMDA

    Science.gov (United States)

    González-Valcárcel, J.; Rosenfeld, M.R.; Dalmau, J.

    2011-01-01

    Resumen Introducción La encefalitis por anticuerpos contra el receptor de NMDA (NMDAR) suele desarrollarse como un síndrome característico de evolución multifásica y diagnóstico diferencial amplio. Pacientes Presentamos a 2 pacientes diagnosticadas de encefalitis por anticuerpos NMDAR con un cuadro clínico típico, pero que inicialmente señaló otras etiologías. Discusión La afectación frecuente de pacientes jóvenes con manifestaciones psiquiátricas prominentes indica frecuentemente otras consideraciones diagnósticas; las más frecuentes son las encefalitis virales, los procesos psiquiátricos y el síndrome neuroléptico maligno. Varios síndromes previamente definidos de manera parcial o descriptiva en adultos y pacientes pediátricos probablemente eran casos de encefalitis anti-NMDAR. Conclusiones La encefalitis anti-NMDAR debe considerarse en pacientes jóvenes con manifestaciones psiquiátricas subagudas, movimientos anormales y alteraciones autonómicas. La caracterización clínica e inmunológica de esta enfermedad ha llevado a la identificación de nuevos anticuerpos que afectan a procesos de memoria, aprendizaje, conducta y psicosis. PMID:20964986

  1. Stabilization and immune response of HBsAg encapsulated within poly(lactic-co-glycolic acid) microspheres using HSA as a stabilizer.

    Science.gov (United States)

    Xu, Wenjuan; He, Jintian; Wu, Guanghao; Xiong, Fangfang; Du, Huijuan; Wang, Gaizhen

    2015-12-30

    The aim of this study was to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres containing hepatitis B virus surface antigen (HBsAg) using human serum albumin (HSA) as a stabilizer. Lyophilization and emulsification of HBsAg solution with dichloromethane caused a considerable loss of HBsAg antigenicity. Thus, the effects of HSA and trehalose on HBsAg recovery during lyophilization and emulsification were investigated. Adding HSA to HBsAg solutions significantly improved antigen recovery to >90% during lyophilization and emulsification. The effects of co-encapsulated HSA on the characteristics of the PLGA microspheres and stability of HBsAg released from the microspheres were also investigated. The in vitro release test showed that HBsAg was released from the PLGA microspheres continuously over seventy days. A large amount of released HBsAg was inactive without co-encapsulation of HSA. On the contrary, with HSA co-encapsulation, the released HBsAg retained approximately 90% of its antigenicity. The single injection of the HBsAg-HSA-loaded PLGA microspheres in rats resulted in higher anti-HBsAg IgG and Th1 cytokine levels than the single injection of the HBsAg-loaded microspheres or two injections of the conventional aluminum-adjuvanted HBsAg vaccine. Based on these findings, the HBsAg-HSA-loaded PLGA microspheres could be an effective carrier for HBsAg and form a promising depot system.

  2. Relationship Between HBV-DNA and Pre S1 Antigen in Patients with HBsAg, Anti-HBe and Anti-HBc Positive Hepatitis B%乙型肝炎“小三阳”患者HBV-DNA定量检测与前S1抗原的关系

    Institute of Scientific and Technical Information of China (English)

    石万元; 阚秉辉; 刘克芹; 尹卫东

    2013-01-01

    目的 探讨“小三阳”患者HBV-DNA含量与前S1抗原的关系.方法 收集乙型肝炎“小三阳”患者血清152例,用ELISA方法检测前S1抗原,用实时荧光定量PCR(FQ-PCR)方法检测HBV-DNA.结果 前S1与HBV-DNA同时阳性者74例,二者同时阴性者34例,符合率为71.1%.前S1阳性、HBV-DNA阴性者为30例,HBV-DNA阳性而前S1阴性者为14例.即“小三阳”患者中前S1的检出率为68.4%,HBV-DNA的检出率为57.9%,并且拷贝数高低有差别.结论 “小三阳”患者体内也存在着不同程度的病毒复制.前S1的检测不能代替HBV-DNA,只能作为其补充指标.对于“小三阳”患者,同时检测HBV-DNA定量有助于判断病毒在体内是否复制.%Objective To investigate the relationship between HBV-DNA with pre S1 antigen in patients with HBsAg,Anti-HBe and Anti-HBc positive hepatitis B.Methods The serum express of pre S1 antigen and HBV-DNA in 152 patients with HBsAg,Anti-HBe and Anti-HBc positive hepatitis B were detected by ELISA and PCR(FQ-PCR) respectively.Results 74 cases of samples were both pre S1 and HBV-DNA positive,while 34 cases were found Pre S1 and HBV-DNA negative.The positive coincidence rate between Pre S1 and HBV-DNA was 71.1%.There were 30 cases with pre S1 positive and HBV-DNA negative,while 14 cases had HBV-DNA positive and pre S1 negative.The detection rate of pre S1 and HBV-DNA in patients with HBsAg,Anti-HBe and Anti-HBc positive hepatitis B were 68.4% and 57.9%,respectively.Conclusion There are virus replications of different degrees in patients with HBsAg,Anti-HBe and Anti-HBc positive hepatitis B.The detection of Pre S1 can not replace HBV-DNA detection,and it could be only used as a supplementary index.The HBV-DNA quantitative detection could facilitate to judge whether virus in-vivo replicate or not.

  3. Anti-tissue transglutaminase antibodies (IgA and IgG in both Crohn's disease and autoimmune diabetes Anticuerpos (IgA e IgG antitransglutaminasa tisular en la enfermedad de Crohn y diabetes autoinmune

    Directory of Open Access Journals (Sweden)

    Virgínia Lúcia Ribeiro-Cabral

    2011-09-01

    Full Text Available Objective: a strong association has been observed between celiac disease, generally its silent clinical form, and autoimmune disorders. A potential correlation with inflammatory bowel disease has also been suggested. Anti-tissue transglutaminase antibodies have been detected in Crohn's disease. We investigated the prevalence of celiac disease in patients with autoimmune diabetes and in Crohn's disease patients and also evaluated the correlation between anti-transglutaminase antibody positivity and the clinical status of these diseases. Methods: anti-tissue transglutaminase and anti-endomysium antibodies were assessed by enzyme-linked immunosorbent assay and indirect immunofluorescence, respectively. Upper digestive endoscopy and duodenal biopsy were indicated for cases with positive serology. Results: anti-transglutaminase antibodies were detected in five diabetic patients (prevalence of 11.1%, only one serum sample was positive for IgG isotypes. Nine of thirty-three patients with Crohn's disease had low positive levels for IgA anti-transglutaminase. Anti-endomysium antibodies were detected only in celiac patients. Celiac disease was confirmed in all diabetic patients submitted to duodenal biopsies who presented both anti-transglutaminase and anti-endomisyum antibodies positivity. In Crohn's disease, its clinical status and the diagnosis of celiac disease were not associated with positive anti-transglutaminase result. Conclusions: the prevalence of celiac disease was high in diabetic patients. Anti-tissue transglutaminase antibodies were sensitive and specific markers of celiac disease in this diabetic group, while these antibodies were of limited value for celiac disease screening in patients with Crohn's disease.

  4. Haptenos, conjugados y anticuerpos para el fungicida pirimetanil

    OpenAIRE

    Mercader Badia, Josep Vicent; Abad Fuentes, Antonio; Abad Somovilla, Antonio; Agulló, Consuelo

    2012-01-01

    [ES] La presente invención se refiere a haptenos, conjugados, derivados marcados y anticuerpos para pirimetanil. Así mismo, la presente invención también se refiere al uso de los derivados marcados de pirimetanil como antígenos de ensayo o inmunógenos para obtener anticuerpos de este fungicida; y al uso de los derivados marcados de pirimetanil como antígenos de ensayo. Además, la presente invención también se refiere a un método de análisis de pirimetanil utilizando los anticuerpos obtenidos,...

  5. Haptenos, conjugados y anticuerpos para el fungicida pirimetanil

    OpenAIRE

    Mercader Badia, Josep Vicent; Abad Fuentes, Antonio; Abad Somovilla, Antonio; Agulló, Consuelo

    2012-01-01

    La presente invención se refiere a haptenos, conjugados, derivados marcados y anticuerpos para pirimetanil. Así mismo, la presente invención también se refiere al uso de conjugados de pirimetanil como antígenos de ensayo o inmunógenos para obtener anticuerpos de este fungicida; y al uso de los derivados marcados de pirimetanil como antígenos de ensayo. Además, la presente invención también se refiere a un método de análisis de pirimetanil utilizando los anticuerpos obtenidos, en ocasiones jun...

  6. Obtención de anticuerpos monoclonales de ratón contra proteasa de cisteína 5 recombinante de Entamoeba histolytica

    Directory of Open Access Journals (Sweden)

    Juanita Trejos S.

    2012-08-01

    Full Text Available Objetivo. Obtener anticuerpos monoclonales de ratón contra la proteasas de cisteína 5 (EhCP5 de Entamoeba histolytica. Materiales y métodos. Se inmunizaron ratones BALB/c por vía intraperitoneal con adyuvante de Freund completo e incompleto con la proteína recombinante EhCP5 obtenida a partir del cultivo de E.coli DH5α trasfectada con el vector recombinante pJC45 que expresa dicha proteína. Se seleccionó el animal con mejor respuesta de anticuerpos. Al cual se le extrajo su bazo como fuente de linfocitos B, los cuales se fusionaron utilizando PEG con células de mieloma de ratón SP2-0/Ag14. Se procedió a selección de los hibridomas y a la evaluación de los sobrenadantes de las colonias que crecieron a los 7 días mediante ELISA. Los hibridomas con valores más altos de anticuerpos específicos contra la proteína EhCP5r se seleccionaron, y los clones obtenidos por diluciones limitantes fueron expandidos. Resultados. A partir de un clon secretor estable se purifico el anticuerpo monoclonal anti EhCP5r del isotipo IgG1 por cromatografía de afinidad con proteína G. Los clones fueron expandidos in vivo e in vitro. Con el anticuerpo purificado se diseñaron tres sistemas de captura para evaluar la aplicabilidad del anticuerpo monoclonal anti EhCP5r como método inmunodiagnóstico. Conclusiones. Se logro la producción de un anticuerpo monoclonal específico contra EhCP5r que permite diferenciar Entamoeba histolytica de Entamoeba dispar.

  7. Inmunofluorescencia con Crithidia luciliae para la detección de anticuerpos anti-ADN: Imágenes atípicas y su relación con enfermedad de Chagas y leishmaniasis Immunofluorescence assay with Crithidia luciliae for the detection of anti-DNA antibodies: Atypical images and their relationship with Chagas’ disease and leishmaniasis

    Directory of Open Access Journals (Sweden)

    Gloria Griemberg

    2006-02-01

    Full Text Available Los anticuerpos anti-ADN nativo pueden detectarse por inmunofluorescencia indirecta con Crithidia luciliae, observándose tinción fluorescente anular del kinetoplasto que contiene ADN de doble cadena. En algunos casos pueden observarse imágenes fluorescentes en flagelo, membrana y corpúsculo basal, consideradas atípicas. Como C. luciliae pertenece a la familia Trypanosomatidae, que incluye patógenos para el hombre como Trypanosoma cruzi y Leishmaniaspp., se consideró que las imágenes atípicas pudieran deberse a reacciones cruzadas. Se realizaron estudios serológicos para Chagas a 105 muestras provenientes de zona endémica (Corrientes y no endémica (Buenos Aires para T. cruzi que presentaban imágenes atípicas con C. luciliae. La serología para Chagas resultó positiva en el 64.7% de las muestras de Buenos Aires y en el 78.3% de las de Corrientes que presentaban frente a C. luciliae imagen conjunta de membrana y flagelo. No presentaron la imagen conjunta ninguna de las muestras de dadores de sangre normales, ni de pacientes con enfermedades del tejido conectivo, excepto dos con lupus que también eran chagásicos. Todas las muestras de pacientes chagásicos analizadas frente a C. luciliae presentaron la imagen conjunta. Se estudiaron también 46 muestras de pacientes con leishmaniasis, 28 de ellos coinfectados con T. cruzi. La imagen conjunta se observó en el 88.0% de las muestras de leishmaniásicos y en el 78.5% de las de coinfectados. Los resultados sugieren que C. luciliae podría ser un sustrato alternativo, económico y de bajo riesgo para el diagnóstico serológico de enfermedad de Chagas, aunque no discrimina la infección por Leishmania. El hallazgo de la imagen conjunta en la detección de anti-ADN nativo señala la conveniencia de realizar en esos pacientes, estudios clínicos y de laboratorio para enfermedad de Chagas y leishmaniasis.Anti-native DNA antibodies can be detected by indirect immunofluorescence assay with

  8. Detección de anticuerpos contra artritis encefalitis caprina (AEC) mediante inmunoelectrotransferencia

    OpenAIRE

    Emiliano Tesoro Cruz; Rafael Hernández González; Alejandro Martínez Rodríguez; Hugo Ramírez Álvarez; María Elena Trujillo Ortega; Roberto Kretschmer Schmid; Álvaro Aguilar Setién

    2003-01-01

    En México la artritis encefalitis caprina (AEC) está presente, pero se ignora la frecuencia y prevalencia. El objetivo del presente trabajo fue evaluar la técnica de inmunoelectrotransferencia o western blot (WB) para la detección de anticuerpos anti-VAEC en sueros caprinos. Se evaluaron 50 sueros de cabras nativas seleccionadas al azar (25 de Amazcala, Querétaro, y 25 de Cuautitlán, Estado de México) por ensayo inmunoenzimático (ELISA) y WB. El criterio de positividad para WB fue el propuest...

  9. Influencia del bloqueo del factor de necrosis tumoral-alfa sobre los niveles del factor reumatoide y anticuerpos anti-péptidos cíclicos citrulinados en pacientes con artritis reumatoide

    OpenAIRE

    Martínez Estupiñán, Lina Patricia

    2015-01-01

    La respuesta clínica a los anti-TNF-α en los pacientes con Artritis Reumatoide (AR) es muy variable, en muchos casos se logra la remisión de la enfermedad, sin embargo una cantidad sustancial de pacientes no responden y persisten con actividad de la enfermedad o presentan recaídas a pesar del tratamiento. Los marcadores clínicos, radiológicos, serológicos y genéticos disponibles para el diagnóstico y seguimiento de la enfermedad tienen un valor limitado a la hora de predecir de manera precisa...

  10. Influencia del bloqueo del factor de necrosis tumoral-alfa sobre los niveles del factor reumatoide y anticuerpos anti-péptidos cíclicos citrulinados en pacientes con artritis reumatoide

    OpenAIRE

    Martínez Estupiñán, Lina Patricia

    2016-01-01

    La respuesta clínica a los anti-TNF-α en los pacientes con Artritis Reumatoide (AR) es muy variable, en muchos casos se logra la remisión de la enfermedad, sin embargo una cantidad sustancial de pacientes no responden y persisten con actividad de la enfermedad o presentan recaídas a pesar del tratamiento. Los marcadores clínicos, radiológicos, serológicos y genéticos disponibles para el diagnóstico y seguimiento de la enfermedad tienen un valor limitado a la hora de predecir de manera precisa...

  11. Immune effect of different hepatitis A vaccine and emergency vaccination of appropriate technology study(1)Analysis on the results of anti-HAV,HBsAg,and ALT of 829 children at age of 4 to 10 in Huadian city%不同甲型肝炎疫苗免疫效果和应急接种适宜技术研究(一)桦甸市829名4~10岁儿童抗HAV、HBsAg和ALT检测结果分析

    Institute of Scientific and Technical Information of China (English)

    王洪海; 范学彬; 姜桂秋; 王艳萍; 刘娟; 陈超; 周剑惠; 曹风瑞; 潘飞

    2011-01-01

    目的 对桦甸市829名儿童的抗HAV、HBsAg和ALT检测结果进行分析,从而确定健康人群.方法 采用整群随机抽样的方法,对1~3年级学生和学前班儿童进行体检和问卷调查,选择既往无甲肝病史和甲肝疫苗接种史的人群作为研究对象,检测其血液中抗HAV、HBsAg和ALT的情况.结果 通过对829名学生进行检测,3项均为正常者为710名.结论 通过检测,确定了健康人群,为评价疫苗的安全性、免疫原性,比较儿童接种不同类型甲肝疫苗后不同时间抗体水平变化,以及应急接种方案确定了研究对象.%Objective To analysis the testing results of anti - HAV, HBsAg and ALT of 829 children in Huadian city, consequently, made sure the healthy population. Method Adopting randomly chester sampling method, students of grade 1 to grade 3 and preschool children had physical examination and questionnaires, selecting the people with no previous hepatitis A history and no hepatitis A vaccination history as research objects, testing anti - HAV, HBsAg and ALT in their blood. Results Examined 829 children, in them, 710 were normal. Conclusions By detection, we made sure the healthy population, and provided research objects for evaluation vaccine safety, immunogenicity, compared antibody levels of children at different time of different hepatitis A vaccination types, and emergency vaccination program

  12. Enhancement of antibody production to hepatitis B surface antigen by anti-idiotypic antibody.

    OpenAIRE

    Kakumu, S; Murase, K.; A Tsubouchi; Yoshioka, K.; Sakamoto, N.

    1986-01-01

    Studies were undertaken to determine whether anti-idiotypic antibody (anti-Id) against antibody to hepatitis B surface antigen (anti-HBs) could modulate in vitro anti-HBs production by human peripheral blood mononuclear cells stimulated with pokeweed mitogen. Peripheral blood mononuclear cells from patients positive for serum anti-HBs produced significantly increased amounts of anti-HBs by the addition of IgG fraction of anti-anti-HBs as well as purified HBsAg in a soluble form when compared ...

  13. The Lumipulse G HBsAg-Quant assay for screening and quantification of the hepatitis B surface antigen.

    Science.gov (United States)

    Yang, Ruifeng; Song, Guangjun; Guan, Wenli; Wang, Qian; Liu, Yan; Wei, Lai

    2016-02-01

    Qualitative HBsAg assay is used to screen HBV infection for decades. The utility of quantitative assay is also rejuvenated recently. We aimed to evaluate and compare the performance of a novel ultra-sensitive and quantitative assay, the Lumipulse assay, with the Architect and Elecsys assays. As screening methods, specificity was compared using 2043 consecutive clinical routine samples. As quantitative assays, precision and accuracy were assessed. Sera from 112 treatment-naïve chronic hepatitis B patients, four patients undergoing antiviral therapy and one patient with acute infection were tested to compare the correlations. Samples with concurrent HBsAg/anti-HBs were also quantified. The Lumipulse assay precisely quantified ultra-low level of HBsAg (0.004 IU/mL). It identified additional 0.98% (20/2043) clinical samples with trance amount of HBsAg. Three assays displayed excellent linear correlations irrespective of genotypes and S-gene mutations (R(2)>0.95, PLumipulse assay did not yield higher HBsAg concentrations in samples with concomitant anti-HBs. Compared with other assays, the Lumipulse assay is sensitive and specific for detecting HBsAg. The interpretation of the extremely low-level results, however, is challenging. Quantitative HBsAg results by different assays are highly correlated, but they should be interpreted interchangeably only after conversion to eliminate the biases.

  14. Obtención de un conjugado anti IgG de ratón - FITC mediante la tecnología IgY para uso como anticuerpo secundario en la detección de antígenos de superficie celular

    Directory of Open Access Journals (Sweden)

    Esteban J. Gutiérrez Calzado

    2007-01-01

    Full Text Available Desde el año 1996, el Centro Europeo para la Validación de Métodos Alternativos recomendó el uso de la IgY como sustituto de la IgG de mamíferos con el objetivo de reducir al mínimo las situaciones de daño que se les causa a los animales que se someten a la producción de anticuerpos (Acs. En 1999, la tecnología IgY se aprobó por la Oficina Federal Veterinaria del gobierno suizo como un método alternativo para sustentar el cuidado y bienestar de los animales. Muchas de las publicaciones relacionadas con IgY demuestran las características desde el punto de vista bioquímico de esta inmunoglobulina que la hace tomar ventajas sobre la IgG, entre las que refiere que los complejos inmunes que contienen Acs aviares, no pueden interactuar con los receptores Fc o del complemento sobre las células, por lo que el reemplazamiento de los Acs de mamíferos por sus similares en gallinas pudieran evitar interferencias en muchos ensayos diagnósticos. Este conocimiento ha sido la base objetiva de este trabajo que consistió en la obtención de Acs IgY anti IgG de ratón con elevado grado de pureza, los que fueron conjugados por unión covalente vía grupos amino primarios libres al isotiocianato de fluoresceína (FITC. Estos conjugados mostraron poseer una razón molar FITC/proteína en intervalos reportados para un buen desempeño. La dilución óptima de trabajo obtenida para ellos fue muy adecuada y demostró su utilidad como Acs secundarios en el conteo de antígenos de superficie celular linfocitario humano por sus resultados comparables con los reactivos similares de origen mamífero ensayados en paralelo.

  15. Diseño de un algoritmo para la inclusión de unidades de bancos de sangre positivas para Anti-HBc y sin infección por el virus de la hepatitis B

    Directory of Open Access Journals (Sweden)

    Sandra Vega

    2016-06-01

    Full Text Available Introducción: Una de las mayores causas de descarte de unidades transfusionales en los bancos de sangre es la infección por el virus de la hepatitis B (VHB. Objetivo: Diseñar un algoritmo estadístico con base en los títulos para anti-HBs y anti-HBc contra el VHB en sueros de donantes de sangre, con el fin de evitar el descarte de unidades sanguíneas de donantes que resolvieron la infección por el virus. Materiales y métodos: 700 alícuotas de suero de donantes del año 2015 fueron suministradas por el Hemocentro del Hospital universitario de Santander (HUS para realizar titulaciones de anti-HBs y anti-HBc. Se busca cuantificar los títulos de anticuerpos se realizará en sueros utilizando ensayos inmunoenzimáticos competitivo (ELISA, siguiendo el protocolo de la casa comercial Diapro. Adicionalmente, se realizará extracción de ácido desoxirribonucleico (ADN con el método de columnas de silica gel (Ultra Sense kit Virus QIAamp a partir de suero siguiendo el protocolo de la casa comercial. El material genético del virus será amplificado por ensayo de PCR anidado utilizando dos tipos de cebadores; un par externo (HBS1 hacia adelante y HBS2 inversa y un par interno (HBS3 hacia adelante y HBS4 inversa, siguiendo el protocolo previamente estandarizado por nuestro laboratorio y obteniendo un producto final de 1100 pares de base. Se establecerá el punto de corte de acuerdo, con lo cual se procederá a crear un algoritmo estadístico utilizando el método de análisis por correspondencia con las variables “Anti-HBc-anti-HBs y DNA viral” teniendo en cuenta cada una de las modalidades de la tabla, cercanía/lejanía entre los puntos calculados (punto de corte. Resultados: Mediante la cuantificación de anticuerpos anti-HBs y anti-HBc se determinará un punto de corte en las unidades de sangre que no tengan el DNA viral siendo estas útiles en caso de llegar a ser transfundidas. Conclusiones: En Santander existen aproximadamente 2’061

  16. Prevalencia de anticuerpos antitreponémicos y características sociodemográficas de la población mexicana adulta en el año 2000 Anti-Treponema pallidum seroprevalence and sociodemographic characteristics in Mexican adult population, 2000

    Directory of Open Access Journals (Sweden)

    Carlos J Conde-González

    2007-01-01

    Full Text Available OBJETIVO: Determinar la frecuencia de anticuerpos antitreponémicos en mexicanos adultos de ambos sexos y las características de la población relacionadas con la infección sifilítica. MATERIAL Y MÉTODOS: Se analizaron para anti-Treponema pallidum 12 010 sueros seleccionados de forma aleatoria de la Encuesta Nacional de Salud 2000, con aplicación de un algoritmo conformado por técnicas serológicas treponémicas y no treponémicas, de tamizaje y confirmatorias, y se realizó un análisis estadístico para identificar los factores vinculados con la seropositividad. RESULTADOS: La seroprevalencia de anticuerpos antitreponémicos fue de 3.1% (IC95% 2.5-3.7. Las características relacionadas de modo significativo con la exposición a la infección fueron la mayor edad (RM 3.3, sexo masculino (RM 1.3, residencia en la región geográfica sur del país (RM 1.7, vivir en unión libre (RM 2.7 o ser viudo (RM 1.8, inicio de relaciones sexuales a edad temprana (RM 6.3 y, en las mujeres en particular, se identificó el antecedente de mortinatos (RM 1.8 y el informe de falta de uso del condón como método anticonceptivo (RM 14.1. CONCLUSIONES: Los hallazgos del estudio demuestran que la magnitud del antecedente de la infección por el treponema en la población mexicana adulta es considerablemente mayor respecto del que permite calcular la notificación oficial de casos de la enfermedad. Como sucede con otras infecciones de transmisión sexual, aspectos sociodemográficos, de género y las prácticas sexuales no seguras incrementan la transmisión del agente etiológico. En México existe la necesidad de reforzar la vigilancia de la infección sifilítica a través de un diagnóstico oportuno y eficaz de los casos, más su adecuado tratamiento, para coadyuvar al control del padecimiento.OBJECTIVE: To measure for the first time in a population based study the prevalence of antibodies against Treponema pallidum among the Mexican adult population and

  17. Distribución heterogénea de la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre en Puebla, México Heterogeneous distribution of the prevalence of anti-Trypanosoma cruzi antibodies among blood donors in the State of Puebla, Mexico

    Directory of Open Access Journals (Sweden)

    Víctor M Monteón

    2005-04-01

    Full Text Available OBJETIVO: Identificar la seroprevalencia de anticuerpos anti Trypanosoma cruzi (Ac anti-T. cruzi en donadores de sangre que habitan en ámbito rural y suburbano, así como las regiones del estado de mayor riesgo y factores asociados. MATERIAL Y MÉTODOS: Estudio transversal realizado de enero a diciembre de 2003. Se analizaron 2 489 donadores de sangre reclutados en 10 puestos de sangrado del Instituto Mexicano del Seguro Social (IMSS distribuidos en las siete regiones económicas del estado de Puebla, México. Se determinó la seroprevalencia mediante las pruebas serológicas obligatorias del panel viral y, además, para T. cruzi, región de reclutamiento y de origen de los donadores. RESULTADOS: La seroprevalencia de Ac anti-T. cruzi fue de 1.24% (31/2 489 comparable con la obtenida para el virus de la hepatitis C (1.5% y por arriba de la del virus de la inmunodeficiencia humana (0.4% y del antígeno de superficie del virus de la hepatitis B (0.3%. Las regiones de Tehuacán-Sierra Negra y Mixteca fueron las de mayor riesgo con seroprevalencias, por el origen del donador, de 2.6% para T. cruzi, mientras que en los originarios de las regiones Sierra nororiental y Angelópolis no se detectaron casos positivos. Se observó asociación entre ser seropositivo y mayor de 40 años y ser originario de las regiones de Tehuacán-Sierra Negra y Mixteca. CONCLUSIONES: La distribución de seroprevalencia a T. cruzi es heterogénea, oscila desde 0% hasta 2.6%, y se reconoce a Tehuacán-Sierra Negra y Mixteca como las regiones de mayor riesgo.OBJECTIVE: To determine the seroprevalence and associated factors, of antibodies against Trypanosoma cruzi (T. cruzi Ab among blood donors living in rural and suburban areas and risk regions. MATERIAL AND METHODS: A cross-sectional study was conducted from January to December 2003, in 2489 blood donors of seven regions of Puebla, who were evaluated for mandatory viral and T. cruzi serological tests using validated

  18. A novel stop codon mutation in HBsAg gene identified in a hepatitis B virus strain associated with cryptogenic cirrhosis

    Institute of Scientific and Technical Information of China (English)

    Xu Yang; Xiao-Peng Tang; Jian-Hua Lei; Hong-Yu Luo; Yong-Hong Zhang

    2003-01-01

    AIM: HBsAg is the most important serological marker for acute or chronic hepatitis B. Nevertheless, there were reports of HBsAg-negative infection caused by hepatitis B virus in recent years. We had a patient with crytogenic cirrhosis who was negative for HBsAg, positive for anti-HBs and HBeAg.This paper was to explore the pathogenic and molecular basis of the unusual serological pattern.METHODS: HBV serologic markers were qualitatively and quantitatively determined. HBV DNA in serum was qualitatively tested using routine Polymerase chain reaction (PCR), and the viral level was determined with real-time fluorescence quantitative PCR. HBsAg gene was amplified and cloned. Four clones were sequenced. The new genomic sequences were compared with GenBank on the DNA level as well as the protein level.RESULTS: The qualitative results of serological markers were HBsAg(-), anti-HBs(+), HBeAg(+), anti-HBe(-) and anti-HBc(+). The quantitative results of serological marker were HBsAg (S/N): 0.77 (cut off of S/N: ≥2.00), HBeAg (S/N): 56.43 (cut off S/N: ≥2.10), anti-HBc (S/Co): 2.03 (cut off of S/Co: ≤ 1.00). The viral level was as high as 1.54×109copies/ml. Sequencing of the HBsAg gene clones revealed a unique point mutation at nucleotide 336 (C to A), which resulted in a novel stop codon at aa 61. The novel HBsAg gene stop mutation had not been described.CONCLUSION: The lack of detection of HBsAg in the presence of high viral levels of replication may be caused by the existence of viral genomes harboring point mutations which resulted in stop codon upstream of the″a″ determinant in HBsAg gene.

  19. [Evaluation of high-sensitivity HBsAg quantitative assay for HBV genotype].

    Science.gov (United States)

    Takagi, Kazumi; Tanaka, Yasuhito; Hiramatu, Kumiko; Kani, Satomi; Tatematsu, Kanako; Naganuma, Hatsue; Ueno, Tetsuo; Gotou, Takaaki; Wakimoto, Yukio; Mizokami, Masashi

    2009-07-01

    The clinical implication of the hepatitis B surface antigen (HBsAg) concentrations has been reported in HBV-infected patients during anti-viral treatment. HBV genotypes A and D are ubiquitous and scattered worldwide, especially northern America as well as Europe, whereas genotypes B and C are common in Asia. The aim of this study was to evaluate a new version of the Sysmex HBsAg quantitative kit based on Chemiluminescence Enzyme Immunoassay. Sera collected from 172 patients infected with any of the four major genotypes A to D (HBV/A, n = 18; B, n = 25; C, n = 84; D, n = 45), including the genotype D cases with weak reaction in the previous version of the kit. The new version of the kit having additional monoclonal antibody, showed improved sensitivity compared to the previous version as well as robust correlation with another quantitative HBsAg assay: the Abbot Architect. Observed during lamivudine therapy, increase in HBsAg and HBV DNA concentrations preceded the aminotransferase (ALT) elevation associated with drug-resistant HBV variant emergence (breakthrough hepatitis). In conclusion, reliability of the Sysmex HBsAg quantitative assay was confirmed for the four HBV genotypes common worldwide. Monitoring of serum HBsAg concentrations in addition to HBV DNA quantification, is helpful in evaluation of the response or resistance to anti-viral therapy.

  20. 血清抗-HBs定量检测在评价儿童乙肝疫苗接种效果方面的价值%Quantitative anti-HBs test application in evaluation of HBV vaccine inoculating on children

    Institute of Scientific and Technical Information of China (English)

    唐跃华; 谢健敏; 刘建辉

    2006-01-01

    目的通过检测接种过乙肝疫苗但抗-HBs定性检测阴性儿童血清中抗-HBs的含量,探讨定量检测抗-HBs在评价儿童乙肝疫苗接种效果方面的价值.方法采用微粒子免疫吸附试验的原理在AXYMS全自动免疫分析仪上定量检测全程免疫过乙型肝炎疫苗而酶联免疫吸附(ELISA)定性检测抗-HBs(-)的76名儿童血清标本中抗-HBs含量.结果76份ELISA定性检测抗-HBs(-)的标本中有41份抗-HBs>10mIU/ml,22份抗-HBs<10mIU/ml,13份抗-HBs<2.1mIU/ml.结论ELISA定性检测抗-HBs(-)的血清中仍有54.0%抗-HBs含量能够达到预防乙肝病毒感染的水平.因此,仅凭ELISA定性检测抗-HBs不能准确判断乙型肝炎疫苗的免疫效果,抗-HBs定量检测在评价疫苗接种效果和确定疫苗再接种方案方面有不可取代的价值.

  1. 母源性抗-HBs对新生儿接种乙型肝炎疫苗的远期影响%Long-term Influence of Maternal Anti-HBs On the Antibody Response to Hepatitis B Vaccine in Infants

    Institute of Scientific and Technical Information of China (English)

    骆超; 武巧珍; 何凤香; 许碧云; 周乙华; 胡娅莉

    2010-01-01

    目的:观察新生儿体内母源性乙型肝炎表面抗体(抗-HBs)对乙型肝炎疫苗接种后抗体应答的远期影响.方法:2006年10月-2007年1月在南京大学医学院附属鼓楼医院产前检查并住院分娩的单胎足月妊娠妇女中,选择抗-HBs阳性孕妇32例,抗-HBs阴性孕妇32例,其新生儿均按0、1、6方案接种重组(酵母)乙型肝炎疫苗,检测两组儿童乙型肝炎疫苗第3针接种后2年血清抗-HBs浓度.结果:新生儿接种乙型肝炎疫苗第3针后2年,母源性抗-HBs阴性组与阳性组儿童抗-HBs阳性率分别为90.6%与87.5%,其抗-HBs几何平均浓度分别为73.48 mIU/ml与75.49 mIU/ml,两组间抗-HBs阳性率与GMC的差异均无统计学意义(P=1.000,P=0.778);6例母源性抗-HBs>1000 mIU/ml儿童中1例抗-HBs转阴(16.7%),而母源性抗-HBs<1000 mIU/ml组和母源性抗-HBs组的儿童抗-HBs转阴率分别仅为7.69%和11.1%,但差异无统计学意(P=0.811).结论:新生儿目前按照0,1,6方案接种乙型肝炎疫苗,能够有效保护其抵抗乙型肝炎病毒的感染;母源性抗-HBs对新生儿接种乙型肝炎疫苗后抗体应答可能不产生远期的影响,但仍需大样本进一步研究.

  2. Detection of serum blocking factors and antibodies to the albumin receptor on HBsAG particles in healthy persons and patients with liver diseases.

    Directory of Open Access Journals (Sweden)

    Tsuji,Takao

    1984-04-01

    Full Text Available An enzyme-linked immunosorbent assay (ELISA for the detection of serum blocking factors (BF, or antibodies to the albumin receptor on HBsAg particles, was developed, and its clinical usefulness was examined in healthy persons and patients with liver diseases. Thirteen of 80 anti-HBs-positive female (16.3% had BF, but all 25 male anti-HBs-positive, 41 female and 32 male anti-HBs-negative subjects were negative for BF. The activity of BF in BF-positive cases was not associated with the positive reciprocal hemagglutination titer of anti-HBs. For a neutralization test of BF, the BFs from 5 cases were absorbed with IgG-immunobeads. It was determined that these IgG-BFs were antibodies to the albumin receptors on HBsAg particles. No significance between positive-BF and abnormal S-GPT levels was recognized. These results suggest that the present test for the detection of BF, or anti-albumin receptor antibody, different from anti-HBs, might be useful for diagnosis of hepatitis B and as a marker for HB virus.

  3. Analysis of Viremia and Clinical Significance in Different Serological Patterns of HBV Infection Associated with Anti-HBs%抗-HBs阳性不同血清学模式病毒学及临床意义分析

    Institute of Scientific and Technical Information of China (English)

    黄利华; 黄祖瑚; 蒋耀明; 刘海肃

    2001-01-01

    目的:通过对抗-HBs阳性不同血清学模式病毒学和临床意义的分析,了解抗-HBs阳性不同血清学模式的特点,探讨其形成机理及抗-HBs的作用.方法:采用Abbott和PCR定量、PCR定性方法分别检测抗-HBs阳性不同血清学模式病人的HBV血清标志物和HBVDNA.结果:抗-HBs、抗-HBe、抗-HBc阳性组与其它模式组比较,其抗-HBs值显著升高(P<0.05);H-BsAg、抗-HBs、HBeAg阳性组与其它模式组比较,其HBV DNA含量显著增高(P<0.01);定性检测HBV DNA阳性组与HBV DNA阴性组比较抗-HBs值,前者抗-HBs值显著降低(P<0.05);抗-HBs阳性不同血清学模式病人生化指标比较无显著性差异(P>0.05);抗-HBs阳性不同血清学模式病人比较其肝炎临床类型的构成有非常显著性差异(P<0.005):即抗-HBs、抗-HBe、抗-HBc阳性组主要表现为急性肝炎,HBsAg、抗-HBs、HBeAg阳性组与HBsAg、抗-HBs阳性组主要表现为慢性肝炎.结论:抗-HBs阳性时HBV处于复制水平,但并不标志HBV复制的停止,仅预示病人感染相和恢复相的动态消长过程.如抗-HBs水平不断提高,则感染相向稳定的恢复相发展.同时从肝功能损伤的程度及肝炎临床类型分析,提示抗-HBs引发的免疫效应最终使部分病人由感染相进入恢复相.

  4. A prospective follow - up study on the HBV - infected cases with anti - HBs positive sera%抗-HBs阳性HBV感染者5年前瞻性随访观察

    Institute of Scientific and Technical Information of China (English)

    黄利华; 张英

    2007-01-01

    目的 观察乙肝表面抗体(抗-HBs)阳性HBV感染者5年疾病演变.方法 对62例抗-HBs阳性者检测5年前后血清乙肝病毒标志物(HBVM)、HBVDNA水平及观察临床演变.结果 5年后肝硬化、肝癌发生率分别11.3%、4.8%,死亡5例见于123、12阳性模式组,123、12、245阳性模式5年后仍有48.1%、66.7%、61.1%保持原模式不变,临床类型构成比有显著性差异,5年后抗-HBs和HBVDNA水平总趋势下降,123模式HBVDNA定量显著下降,12模式抗-HBs水平显著下降.结论 HBVDNA和血清抗-HBs水平高低是预示疾病转归的重要指标.

  5. Frecuencia de anticuerpos contra el virus C de la hepatitis en pacientes con cirrosis hepática en Yucatán, México Frequency of antibodies against the hepatitis C virus in patients with hepatic cirrhosis in Yucatan, Mexico

    Directory of Open Access Journals (Sweden)

    Renán A Góngora-Biachi

    2003-10-01

    Full Text Available OBJETIVO: En este estudio reportamos la prevalencia de anticuerpos contra el virus C de la hepatitis (Ac-VCH en un grupo de pacientes con cirrosis hepática (CH. MATERIAL Y MÉTODOS: Se hizo un estudio prospectivo, transversal y descriptivo, de marzo de 1998 a mayo de 1999. Se estudiaron a 153 pacientes (117 (76% hombres y 36 (24% mujeres con diagnóstico de CH, que eran atendidos en el Hospital General Agustín O' Horan y en el Centro de Investigaciones Regionales Doctor Hideyo Noguchi, en la ciudad de Mérida, Yucatán, México. Se aplicó un cuestionario con datos clínico-epidemiológicos y se determinó la presencia de Ac-VCH (ELISA de 2ª generación y RIBA-2 para confirmar el diagnóstico a cada paciente. Se determinó también el antígeno de superficie de la hepatitis B (AgsHB y anticuerpos contra el antígeno central de la hepatitis B (Anti-HBc mediante el método de ELISA. La presencia de Ac-VCH fue relacionada con las variables epidemiológicas de los sujetos. La prevalencia de anti-HCV y la frecuencia de características se compararon entre los pacientes positivos y negativos con las pruebas de c² y exacta de Fisher. RESULTADOS: El 32% de los pacientes con CH (35/117 (30% hombres y 14/36 (39% mujeres fueron positivos para los Ac-VCH. El alcoholismo estuvo presente en todos los hombres serorreactivos y en ninguna de las mujeres positivas (pOBJECTIVE: To report the prevalence of antibodies against the hepatitis C virus (anti-HCV in a group of patients with hepatic cirrhosis (HC. MATERIAL AND METHODS: A prospective transversal and descriptive study was carried out from March 1998 to May 1999. Study subjects were 153 patients; 117 (76% male and 36 (24% female, diagnosed with HC. They were attended at the General Hospital Agustín O' Horan and at Regional Research Center Doctor Hideyo Noguchi, in Merida, Yucatan, Mexico. A clinical-epidemiologic questionnaire completed by interview was used for data collection. Anti-HCV were detected

  6. Antisperm autoantibodies and infertility Anticuerpos antiespermatozoides e infertilidad

    Directory of Open Access Journals (Sweden)

    Jorge Eliécer Ossa Londoño

    1997-02-01

    Full Text Available Fourteen human infertile couples were studied along with seven fertile control couples in order to determine the presence of antisperm au. toantibodies and to correlate results with their clinical reproductive status, spermogram parameters and the quality of cervical mucus. Immunoglobulins of IgG and IgA isotypes were evaluated on spermatozoa by the direct immunobead technique and in serum and cervical mucus by the indirect method. The following differences were found: Nine of 14 male patients but only 1 of 7 male controls had IgG on spermatozoa (p= 0.042 and OR= 10.8. Seven out of 14 male patiens and 1 of 7 controls were positive for IgA on spermatozoa (p= 0.12. Infertile males had a higher frequency of abnormal spermogram parameters as compared to controls (p= 0.0029 and OR= 3.32. Interestingly, the presence of only IgG in serum appeared to be a "protective factor" against IgG on sperm; only one out of the 16 positive males contradicted this observation. Para determinar la presencia de anticuerpos antiespermatozoides y correlacionarla con la situación reproductiva, el espermograma y la calidad del moco cervical, se estudiaron 7 parejas sanas, con hijos, y 14 infértiles. Se determinaron por las técnicas directa e indirecta de inmunoesferas los anticuerpos de los isotipos IgG e IgA en espermatozoides, suero y moco cervical. En 9 de los 14 hombres infértiles y en 1 de los 7 fértiles se encontraron anticuerpos IgG en la superficie de los espermatozoides (p= 0.042 y una Razón de disparidad [RD] = 10.8 y 8 de éstos también tuvieron IgA en los espermatozoides. El número de pruebas alteradas en el espermograma de los hombres infértiles fue significativamente mayor (p= 0.0029 y RD = 3.32. Tanto en los hombres infértiles como en los fértiles la presencia de sólo IgG en el suero parece ser un "factor de protección" contra IgG en los espermatozoides; de los 16 hombres que presentaron anticuerpos, fuera en el suero o en los espermatozoides, s

  7. Persistence of protective anti-HBs antibody levels and anamnestic response to HBV booster vaccination: A cross-sectional study among healthcare students 20 years following the universal immunization campaign in Italy.

    Science.gov (United States)

    Dini, Guglielmo; Toletone, Alessandra; Barberis, Ilaria; Debarbieri, Nicoletta; Massa, Emanuela; Paganino, Chiara; Bersi, Francesca; Montecucco, Alfredo; Alicino, Cristiano; Durando, Paolo

    2017-02-01

    Vaccination against Hepatitis B Virus (HBV) became mandatory in Italy for all newborns and 12 years-old individuals in the 1991. The immunogenicity of HBV vaccine and the effectiveness of the universal immunization strategy have been widely demonstrated. However the need to assess the antibody concentrations above the well known serological correlate of protection for HBV infection (≥10 mIU/mL), established in individuals immunized with a 3 doses vaccination course, is still recommended in subjects exposed to occupational risks in different settings, particularly the healthcare services. This practice has to be performed during the preventive medical examination, before the worker's exposure to biological hazards, as a fundamental part of Occupational Health Surveillance Programs in several Countries, including Italy: the goal is to assure individual protection, also providing booster doses when needed, after many years following the primary vaccination. During the 2011-2013 period, an observational study was performed in Healthcare students (HCSs) trained at a regional university acute-care hospital in North-Western Italy, properly immunized against HBV during infancy or adolescence, in order to evaluate the persistence of seroprotection and to assess the anamnestic response to booster vaccination. Data from 717 subjects undergoing HbsAg Ab and HBc Ab testing during the preventive medical examination, and receiving a booster dose of HBV vaccine when resulting with a non-protective titer (<10 mIU/mL), were collected and analyzed. Most of the HCSs (74.6%) included in the survey, mean age 24.8 y ( ± 4.6 SD), had received the primary vaccination course during the first year of life (3-5-11 months). Globally, 507 (70.7%) HCSs showed protective antibody titres, and an anamnestic response was observed in more than 95% subjects receiving the booster dose. Our study demonstrated the long-term persistence of protection of HBV vaccine, more than 20 y following the

  8. Persistence of protective anti-HBs antibody levels and anamnestic response to HBV booster vaccination: A cross-sectional study among healthcare students 20 years following the universal immunization campaign in Italy

    Science.gov (United States)

    Dini, Guglielmo; Toletone, Alessandra; Debarbieri, Nicoletta; Massa, Emanuela; Bersi, Francesca; Montecucco, Alfredo; Alicino, Cristiano; Durando, Paolo

    2017-01-01

    ABSTRACT Vaccination against Hepatitis B Virus (HBV) became mandatory in Italy for all newborns and 12 years-old individuals in the 1991. The immunogenicity of HBV vaccine and the effectiveness of the universal immunization strategy have been widely demonstrated. However the need to assess the antibody concentrations above the well known serological correlate of protection for HBV infection (≥10 mIU/mL), established in individuals immunized with a 3 doses vaccination course, is still recommended in subjects exposed to occupational risks in different settings, particularly the healthcare services. This practice has to be performed during the preventive medical examination, before the worker's exposure to biological hazards, as a fundamental part of Occupational Health Surveillance Programs in several Countries, including Italy: the goal is to assure individual protection, also providing booster doses when needed, after many years following the primary vaccination. During the 2011–2013 period, an observational study was performed in Healthcare students (HCSs) trained at a regional university acute-care hospital in North-Western Italy, properly immunized against HBV during infancy or adolescence, in order to evaluate the persistence of seroprotection and to assess the anamnestic response to booster vaccination. Data from 717 subjects undergoing HbsAg Ab and HBc Ab testing during the preventive medical examination, and receiving a booster dose of HBV vaccine when resulting with a non-protective titer (<10 mIU/mL), were collected and analyzed. Most of the HCSs (74.6%) included in the survey, mean age 24.8 y ( ± 4.6 SD), had received the primary vaccination course during the first year of life (3–5–11 months). Globally, 507 (70.7%) HCSs showed protective antibody titres, and an anamnestic response was observed in more than 95% subjects receiving the booster dose. Our study demonstrated the long-term persistence of protection of HBV vaccine, more than 20

  9. Detección de pepsinógeno mediante el uso de antisueros y anticuerpos monoclonales.

    Directory of Open Access Journals (Sweden)

    Oscar Orozco

    2000-02-01

    Full Text Available

    El pepsinógeno (PGA y PGC es una aspartato proteasa secretada por células principales gástricas que a pH ácido se convierte en pepsina. Los niveles séricos de dicha enzima se han postulado como indicadores del estado de la mucosa gástrica ya que dependiendo de la patología de base estos pueden encontrarse elevados o disminuidos. Así, en la gastritis superficial y úlcera duodenal los niveles de PGA se encuentran aumentados, mientras que en la gastritis atrófica y cáncer gástrico se encuentran disminuidos. Se planteó como objetivo de este trabajo producir anticuerpos policlonales y monoclonales anti-pepsinógeno con el fin de ser empleados en el diseño de sistemas de cuantificación de pepsinógeno sérico, ya que los sistemas de cuantificación comerciales son muy costosos y de difícil acceso. Para la producción de los anticuerpos se empleó pepsina A porcina como inmunógeno teniendo en cuenta que presenta una alta homología en la secuencia de aminoácidos (86% con la pepsina A humana y además tienen una estructura secundaria idéntica. Anticuerpos policlonales fueron obtenidos de conejos Nueva Zelanda inmunizados vía subcutánea con pepsina A porcina y los anticuerpos monoclonales a partir de células de bazo de los ratones BALB/c inmunizados con pepsina A vía intraperitoneal. Los anticuerpos tanto policlonales como monoclonales fueron purificados por precipitación con sulfato de amonio y posteriormente cromatografía de intercambio iónico a partir del suero o líquido ascítico respectivamente. Los anticuerpos purificados, tanto policlonales como monoclonales, son capaces de reconocer pepsina porcina y pepsinógeno humano presente en sonicados de biopsias gástricas en ensayos de ELISA y western blot. En ensayos de inmunohistoquímica realizados sobre cortes de biopsias gástricas los anticuerpos monoclonales detectan el pepsinógeno presente en el

  10. Improved cell mediated immune responses after successful re-vaccination of non-responders to the hepatitis B virus surface antigen (HBsAg) vaccine using the combined hepatitis A and B vaccine.

    Science.gov (United States)

    Nyström, Jessica; Cardell, Kristina; Björnsdottir, Thora Björg; Fryden, Aril; Hultgren, Catharina; Sällberg, Matti

    2008-11-01

    We successfully re-vaccinated hepatitis B virus (HBV) vaccine non-responders using a double dose of the combined hepatitis A virus (HAV) and HBV vaccine. The hope was to improve priming of hepatitis B surface antigen (HBsAg)-specific cell mediated immune response (CMI) by an increased antigen dose and a theoretical adjuvant-effect from the local presence of a HAV-specific CMI. A few non-responders had a detectable HBsAg-specific CMI before re-vaccination. An in vitro detectable HBsAg-specific CMI was primed equally effective in non-responders (58%) as in first time vaccine recipients (68%). After the third dose a weak, albeit significant, association was observed between the magnitude of HBsAg-specific proliferation and anti-HBs levels. This regimen improves the priming of HBsAg-specific CMIs and antibodies.

  11. Hepatitis B virus markers in anti-HBc only positive individuals.

    Science.gov (United States)

    Weber, B; Melchior, W; Gehrke, R; Doerr, H W; Berger, A; Rabenau, H

    2001-07-01

    Isolated reactivity to hepatitis B virus (HBV) core antigen (anti-HBc) is observed relatively frequently in immunocompromised individuals, intravenous drug abusers (IVDA), and in the presence of HCV infection. The reason for the lack of HBsAg is not clear. The aim of the present study was to investigate which factors (genetic variability of S gene, low-level HBsAg, and immune complexes may be responsible for the failure of HBsAg detection with commercial HBsAg screening assays. Dilution series of two recombinant HBsAg escape mutants and dilutions of serum samples from chronic HBV carriers with multiple insertions in the a determinant and different HBsAg subtypes were tested with a highly sensitive assay that detects wild-type HBsAg (Elecsys HBsAg, Roche Diagnostics, Penzberg, Germany) and two assays that detect HBV wild-type and escape mutants (Murex HBsAg Version 3, Murex and Enzygnost HBsAg 5.0, Dade Behring, Marburg, Germany). Elecsys HBsAg showed in comparison to Murex HBsAg Version 3 and Enzygnost HBsAg 5.0 a reduced sensitivity for escape mutant detection. On the other hand, the best performance for HBsAg subtype detection was obtained with Elecsys HBsAg. In the second part of the study, a selected panel of isolated anti-HBc reactive (n = 104) serum samples (AxSYM Core) was submitted to testing by Elecsys HBsAg, Murex HBsAg Version 3, Enzygnost HBsAg 5.0, and HBsAg detection after immune complex dissociation (ICD) and anti-HBs determination with two different assays (AxSYM Ausab and Elecsys Anti-HBs). To assess the specificity of anti-HBc test results, all the samples were tested by a second anti-HBc assay (Elecsys Anti-HBc). Quantitative HBV DNA detection was undertaken with a commercially available HBV PCR assay (Amplicor HBV Monitor). HCV infection was present in 65.4% of anti-HBc only reactive individuals. Five AxSYM Core positive samples were negative by Elecsys Anti-HBc. Overall, 15 (14.4%) AxSYM Ausab negative samples gave positive results with Elecsys

  12. HBsAg inhibits the translocation of JTB into mitochondria in HepG2 cells and potentially plays a role in HCC progression.

    Directory of Open Access Journals (Sweden)

    Yun-Peng Liu

    Full Text Available BACKGROUND AND AIMS: The expression of the jumping translocation breakpoint (JTB gene is upregulated in malignant liver tissues; however, JTB is associated with unbalanced translocations in many other types of cancer that suppress JTB expression. No comprehensive analysis on its function in human hepatocellular carcinoma (HCC has been performed to date. We aimed to define the biological consequences for interaction between JTB and HBsAg in HCC cell lines. METHODS: We employed the stable transfection to establish small HBsAg expressing HepG2 cell line, and stably silenced the JTB expression using short hairpin RNA in HepG2 cell line. The effects of JTB and small HBsAg in vitro were determined by assessing cell apoptosis and motility. RESULTS: Silencing of JTB expression promoted cancer cell motility and reduced cell apoptosis, which was significantly enhanced by HBs expression. Expression of HBsAg inhibited the translocation of JTB to the mitochondria. Furthermore, silencing of the JTB resulted in an increase in the phosphorylation of p65 in HepG2 cells and HepG2-HBs cells, whereas HBsAg expression decreased the phosphorylation of p65. The silencing of JTB in HepG2-HBs cells conferred increased advantages in cell motility and anti-apoptosis. CONCLUSION: HBsAg inhibited the translocation of JTB to the mitochondria and decreased the phosphorylation of p65 through the interaction with JTB, After JTB knockdown, HBsAg exhibited a stronger potential to promote tumor progression. Our data suggested that JTB act as a tumor suppressor gene in regards to HBV infection and its activation might be applied as a therapeutic strategy for in control of HBV related HCC development.

  13. 固相反向间接血凝试验检测抗-HBs的抗独特型抗体%A solid phase reverse passive hemagglutinatin for the detection of anti-Id-iotype directed against anti-HBs molecules

    Institute of Scientific and Technical Information of China (English)

    何东风

    2003-01-01

    目的建立固相反向间接血凝试验检测抗-HBs的抗独特型抗体的方法.方法用羊抗人IgG包被聚苯乙烯微孔板(V型),捕捉血清中的抗-HBs的抗独特型抗体(IgG),再加入抗-HBs致敏的血球,观察其凝集结果.结果对正常人血清标本116例以及HBsAg阳性血清标本102例进行了抗-HBs的抗独特型抗体检测,在HBsAg阳性血清标本中共检出抗-HBs的抗独特型抗体阳性标本24例,阳性率为23.7%.结论该方法具有简便、快速,又不需要特殊设备等优点.

  14. Viral hepatitis and rapid diagnostic test based screening for HBsAg in HIV-infected patients in rural Tanzania.

    Directory of Open Access Journals (Sweden)

    Fabian C Franzeck

    Full Text Available BACKGROUND: Co-infection with hepatitis B virus (HBV is highly prevalent in people living with HIV in Sub-Saharan Africa. Screening for HBV surface antigen (HBsAg before initiation of combination antiretroviral therapy (cART is recommended. However, it is not part of diagnostic routines in HIV programs in many resource-limited countries although patients could benefit from optimized antiretroviral therapy covering both infections. Screening could be facilitated by rapid diagnostic tests for HBsAg. Operating experience with these point of care devices in HIV-positive patients in Sub-Saharan Africa is largely lacking. We determined the prevalence of HBV and Hepatitis C virus (HCV infection as well as the diagnostic accuracy of the rapid test device Determine HBsAg in an HIV cohort in rural Tanzania. METHODS: Prospectively collected blood samples from adult, HIV-1 positive and antiretroviral treatment-naïve patients in the Kilombero and Ulanga antiretroviral cohort (KIULARCO in rural Tanzania were analyzed at the point of care with Determine HBsAg, a reference HBsAg EIA and an anti-HCV EIA. RESULTS: Samples of 272 patients were included. Median age was 38 years (interquartile range [IQR] 32-47, 169/272 (63% subjects were females and median CD4+ count was 250 cells/µL (IQR 97-439. HBsAg was detected in 25/272 (9.2%, 95% confidence interval [CI] 6.2-13.0% subjects. Of these, 7/25 (28% were positive for HBeAg. Sensitivity of Determine HBsAg was rated at 96% (95% CI 82.8-99.6% and specificity at 100% (95% CI, 98.9-100%. Antibodies to HCV (anti-HCV were found in 10/272 (3.7%, 95% CI 2.0-6.4% of patients. CONCLUSION: This study reports a high prevalence of HBV in HIV-positive patients in a rural Tanzanian setting. The rapid diagnostic test Determine HBsAg is an accurate assay for screening for HBsAg in HIV-1 infected patients at the point of care and may further help to guide cART in Sub-Saharan Africa.

  15. Quantification of HBsAg: basic virology for clinical practice.

    Science.gov (United States)

    Lee, Jung Min; Ahn, Sang Hoon

    2011-01-21

    Hepatitis B surface antigen (HBsAg) is produced and secreted through a complex mechanism that is still not fully understood. In clinical fields, HBsAg has long served as a qualitative diagnostic marker for hepatitis B virus infection. Notably, advances have been made in the development of quantitative HBsAg assays, which have allowed viral replication monitoring, and there is an opportunity to make maximal use of quantitative HBsAg to elucidate its role in clinical fields. Yet, it needs to be underscored that a further understanding of HBsAg, not only from clinical point of view but also from a virologic point of view, would enable us to deepen our insights, so that we could more widely expand and apply its utility. It is also important to be familiar with HBsAg variants and their clinical consequences in terms of immune escape mutants, issues resulting from overlap with corresponding mutation in the P gene, and detection problems for the HBsAg variants. In this article, we review current concepts and issues on the quantification of HBsAg titers with respect to their biologic nature, method principles, and clinically relevant topics.

  16. Early invasive prenatal diagnosis in HBsAg-positive women

    NARCIS (Netherlands)

    P.M. Grosheide (Pia Maria); H.W.P. Quartero (H. W P); S.W. Schalm (Solko); R.A. Heijtink; C.G.M.L. Christiaens

    1994-01-01

    textabstractFrom 1982 to 1989, pregnant women in two large city hospitals in The Netherlands had serum samples screened for hepatitis B surface antigen (HBsAg). Infants of mothers found to be HBsAg-positive received hepatitis B immune globulin immediately after birth and hepatitis B vaccine in the f

  17. Difference in hTERT Gene Expressions between HbsAg-Positive and HbsAg-Negative Hepatocellular Carcinoma

    Institute of Scientific and Technical Information of China (English)

    GUO Yueqing; ZHOU Xu; LIU Enyu; LI Xingrui; LIU Jinwen; YANG Zhifang; YI Jilin

    2005-01-01

    Summary: To investigate the difference in expression of hTERT gene between HbsAg-positive human hepatocellular carcinoma (HCC) and HbsAg-negative HCC and to explore the relationship between HBV infection and hTERT gene expression in HCC. The expression of hTERT protein in 30 cases of HbsAg positive HCC and 17 cases of HbsAg negative HCC was detected by immunohistochemistry (SP method), and the expression of hTERT mRNA was analyzed by reverse transcription polymerase chain reaction (RT-PCR). t-test, Chi-squared test and cochran- armitage trend test were used to see whether there was an interrelation between HBsAg and hTERT gene in HCC. The expression of hTERT protein was mostly located in plasm and occasionally in the nucleus of liver cancer cells. The positive rate of hTERT protein and hTERT mRNA in HbsAg positive HCC- 93.33 % (28/30) and 83.33 % (25/30) respectively which were much higher than those in HbsAg negative HCC- 52.94 % (9/17), 47.06 % (8/17) (P<0.01) respectively. HbsAg is related to hTERT gene expression in human hepatocellular carcinoma. The hTERT gene activated by the efficacious ingredient of HBV may play an important role in hepatocellular transformation and carcinogenesis.

  18. Manejo de la embarazada con isoinmunización por anticuerpos irregulares

    OpenAIRE

    Fuenzalida C,Javiera; Carvajal C,Jorge A

    2014-01-01

    La isoinmunización eritrocitaria feto-materna se define como la presencia de anticuerpos maternos dirigidos contra antígenos presentes en los glóbulos rojos fetales. Los anticuerpos maternos pueden atravesar la barrera placentaria y provocar hemólisis de los glóbulos rojos fetales produciendo anemia hemolítica e hiperbilirrubinemia, características de la enfermedad hemolítica perinatal (EHP). La principal causa de EHP es la incompatibilidad ABO, seguida de la isoinmunización por RhD; esta últ...

  19. Seguridad post-comercialización de los anticuerpos monoclonales antineoplásicos

    OpenAIRE

    González Iglesias, Verónica

    2016-01-01

    INTRODUCCIÓN: Los primeros anticuerpos monoclonales comercializados en España han sido rituximab, trastuzumab, bevacizumab, cetuximab y panitumumab, en este orden. El estudio de su seguridad en los primeros años postcomercialización es fundamental para definir su relación beneficio/riesgo en las condiciones de uso autorizadas. JUSTIFICACIÓN Y OBJETIVOS: Con el fin de estudiar la seguridad postcomercialización de los anticuerpos monoclonales nos planteamos como objetivos: 1) Analizar las s...

  20. [Clinical evaluation of a novel HBsAg quantitative assay].

    Science.gov (United States)

    Takagi, Kazumi; Tanaka, Yasuhito; Naganuma, Hatsue; Hiramatsu, Kumiko; Iida, Takayasu; Takasaka, Yoshimitsu; Mizokami, Masashi

    2007-07-01

    The clinical implication of the hepatitis B surface antigen (HBsAg) concentrations in HBV-infected individuals remains unclear. The aim of this study was to evaluate a novel fully automated Chemiluminescence Enzyme Immunoassay (Sysmex HBsAg quantitative assay) by comparative measurements of the reference serum samples versus two independent commercial assays (Lumipulse f or Architect HBsAg QT). Furthermore, clinical usefulness was assessed for monitoring of the serum HBsAg levels during antiviral therapy. A dilution test using 5 reference-serum samples showed linear correlation curve in range from 0.03 to 2,360 IU/ml. The HBsAg was measured in total of 400 serum samples and 99.8% had consistent results between Sysmex and Lumipulse f. Additionally, a positive linear correlation was observed between Sysmex and Architect. To compare the Architect and Sysmex, both methods were applied to quantify the HBsAg in serum samples with different HBV genotypes/subgenotypes, as well as in serum contained HBV vaccine escape mutants (126S, 145R). Correlation between the methods was observed in results for escape mutants and common genotypes (A, B, C) in Japan. Observed during lamivudine therapy, an increase in HBsAg and HBV DNA concentrations preceded the aminotransferase (ALT) elevation associated with drug-resistant HBV variant emergence (breakthrough hepatitis). In conclusion, reliability of the Sysmex HBsAg quantitative assay was confirmed for all HBV genetic variants common in Japan. Monitoring of serum HBsAg concentrations in addition to HBV DNA quantification, is helpful in evaluation of the response to lamivudine treatment and diagnosis of the breakthrough hepatitis.

  1. HCV and HBV coexist in HBsAg-negative patients with HCV viremia; possibility of coinfection in these patients must be considered in HBV-high endemic area

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dong Soon [Korea Cancer Center Hospital, Seoul (Korea, Republic of)

    1998-01-01

    Hepatocellular carcinoma (HCC) is one of the most common cancers and is highly associated with HBV infection in Korea. It has been suggested that HCV core protein may impair the polymerase activity of HBV in vitro, potentially lowering HBV titre in coinfected patients. The aim of this study was to confirm the coexistence of HBV viremia in HCV infected patients HCC who have apparent HBsAg seronegativity. The serological profiles of HBV and HCV in 616 patients with HCC were analysed and coinfection rate of HBV and HCV investigated. Sera were obtained from 16 patients who were both anti-HCV and HCV RNA positive but HbsAg negative, and tested for HBV BY PCR. As a control group, sera were obtained from 15 patients with HCC and 30 non-A abd non-B chronic hepatitis patients without HCC; both were anti-HCV, HCV-RNA, and HBsAg negative and tested for HBV PCR. Of 616 patients with HCC, 450 (73.1 %) had current HBV infection, 48 (7.8 %) had anti-HCV antibodies, and nine (1.5 %) had viral markers of both HCV abd HBV by serological profiles. Of 27 the patients with HCV viremia and HBsAg seronegativity, 14 (51.9 %) showed HBV viremia by PCR. In contrast, of the 75 patients in the control group who were both HCV PCR negative and HBsAg negative, five (11.1 %) showed HBV viremia by PCR. The PCR for HBV revealed coexistent HBV viremia in HCV viremia patients, despite HBsAg negativity by EIA. In HBV-endemic areas, the possibility of coinfection of HBV in HBsAg-negative patients with HCV viremia should be considered and molecular analysis for HBV-DNA performed. (author). 18 refs., 4 tabs.

  2. Virus-specific immune response in HBeAg-negative chronic hepatitis B: relationship with clinical profile and HBsAg serum levels.

    Directory of Open Access Journals (Sweden)

    Elisabetta Loggi

    Full Text Available BACKGROUND AIMS: The immune impairment characterizing chronic hepatitis B (cHBV infection is thought to be the consequence of persistent exposure to viral antigens. However, the immune correlates of different clinical stages of cHBV and their relation with different levels of HBsAg have not been investigated. The aim of the present study was to evaluate the relationship between HBV-specific T cells response and the degree of in vivo HBV control and HBsAg serum levels in HBeAg-HBeAb+ cHBV. METHODS: Peripheral blood mononuclear cells from 42 patients with different clinical profiles (treatment-suppressed, inactive carriers and active hepatitis of cHBV, 6 patients with resolved HBV infection and 10 HBV-uninfected individuals were tested with overlapping peptides spanning the entire HBV proteome. The frequency and magnitude of HBV-specific T cell responses was assessed by IFNγ ELISPOT assay. Serum HBsAg was quantified with a chemiluminescent immunoassay. RESULTS: The total breadth and magnitude of HBV-specific T cell responses did not differ significantly between the four groups. However, inactive carriers targeted preferentially the core region. In untreated patients, the breadth of the anti-core specific T cell response was inversely correlated with serum HBsAg concentrations as well as HBV-DNA and ALT levels and was significantly different in patients with HBsAg levels either above or below 1000 IU/mL. The same inverse association between anti-core T cell response and HBsAg levels was found in treated patients. CONCLUSIONS: Different clinical outcomes of cHBV infection are associated with the magnitude, breadth and specificity of the HBV-specific T cell response. Especially, robust anti-core T cell responses were found in the presence of reduced HBsAg serum levels, suggesting that core-specific T cell responses can mediate a protective effect on HBV control.

  3. Screening of Human Antibody Fab Fragment against HBsAg and the Construction of its dsFv Form

    Directory of Open Access Journals (Sweden)

    Leili Jia, Jiyun Yu, Hongbin Song, Xuelin Liu, Weina Ma, Yuanyong Xu, Chuanfu Zhang, Shicun Dong, Qiao Li

    2008-01-01

    Full Text Available The objective of this study was to pursue the techniques involving the screening of the human antibody Fab fragment against hepatitis B virus surface antigen (HBsAg and the construction of its disulfide-stabilized Fv fragment (dsFv. The phage antibody Fab fragments against HBsAg were screened from the human combinatorial immunoglobulin library. Sequence analysis revealed that its heavy chain gene was complete, but the light chain gene was lost. To improve the affinity of the antibody by chain shuffling, a human antibody light chain gene repertoire was generated by reverse transcriptase-polymerase chain reaction (RT-PCR from the human peripheral blood lymphocytes. A phage antibody sub-library was then constructed by inserting the light chain gene repertoire into the phagmid that contained the Fd gene. Five clones with appreciably higher absorbance than that of the original clone were obtained, which indicated that the affinity of the light chain-shuffled phage antibodies was improved. Then, the mutated genes of dsFv against HBsAg were constructed by using PCR-based point mutagenesis method. Purified VH and VL proteins were folded into a 25-kDa protein, designated as anti-HBsAg dsFv. ELISA and competition ELISA revealed that the dsFv maintained the specificity of the Fab by binding to HBsAg, even through with a lower binding activity. These results have facilitated the undertaking of further functional analyses of the constructed dsFv, and may therefore provide an improved technique for the production and application of dsFvs against HBsAg.

  4. Prevalence of hepatitis B surface antigen (HBsAg) in blood donors from Bombay.

    Science.gov (United States)

    Satoskar, A; Ray, V

    1992-01-01

    Analysis of serum samples from 3104 blood donors from Bombay screened for hepatitis B surface antigen (HBsAg) by ELISA. HBsAg was detected in 4.7% of the subjects. Relatives showed a significantly higher prevalence of HBsAg than volunteer donors. There was no significant association between HBsAg positivity and a particular blood group.

  5. Hepatocellular carcinoma HBsAg positive in pregnancy.

    Science.gov (United States)

    Gonçalves, C S; Pereira, F E; de Vargas, P R; Ferreira, L S

    1984-01-01

    The authors present a case of hepatocellular carcinoma diagnosed in a pregnant woman (four months pregnancy). The clinical evolution was complicated because of a severe hypoglicemia and the patient died 12 weeks after admission. The fetus died before a tentative of surgical delivery. The patient was HBsAg positive and five out of eight sons (inclusively the fetus), were HBsAg positive. There was not indication that the pregnancy had enhanced the tumor evolution.

  6. 盐城市1~7岁儿童乙型肝炎病毒表面抗体水平及加强免疫研究%Boosting Vaccination and the Anti-HBs Level of Children Aged 1-7 Years Old in Yancheng City

    Institute of Scientific and Technical Information of China (English)

    陈胤忠; 刘社兰; 吴巨飞; 刘秀兰; 张盐珠; 顾善坤; 丁松林; 汤书霞; 姜仁杰; 沈进进; 朱凤才; 梁争论

    2007-01-01

    目的 了解盐城市1~7岁儿童乙型肝炎(乙肝)病毒表面抗原(HBsAg)阳性率和乙肝病毒表面抗体(抗-HBs)水平,评估实施乙肝疫苗免疫以来保护效果.方法 采用多阶段分层整群抽样方法 ,对1998~2004年出生的儿童进行HBsAg阳性率和抗-HBs水平测定,并对其中的阴性对象进行加强免疫.结果 调查1~7岁儿童2 477人,HBsAg阳性率为0.61%,抗-HBs阳性率为62.4%,各年龄组之间的抗-HBs阳性率和几何平均浓度(GMC)有明显的差异,即随着年龄的增长,抗-HBs阳性率和GMC逐渐下降.对310名抗-HBs阴性对象分别采用重组乙肝疫苗[中国仓鼠卵巢细胞(CHO细胞)]10μg、20μg和重组乙肝疫苗(酵母)5μg、10μg进行加强免疫,结果 显示:重组乙肝疫苗(CHO细胞)20μg组抗-HBs阳性率和GMC最高,分别为96.3%和301.65mIU/ml;重组乙肝疫苗(酵母)5μg的抗-HBs阳性率和GMC最低,分别为90.7%和203.10mIU/ml.结论 盐城市1~7岁儿童HBsAg阳性率显著低于全国平均水平,随着年龄的增长,抗-HBs阳性率和GMC逐渐下降,3岁儿童的抗-HBs阳性率下降至56.6%,有必要对5μg重组乙肝疫苗(酵母)在儿童中的应用效果进行进一步研究,考虑在适当年龄采用10μg以上的重组乙肝疫苗(CHO细胞或酵母)予以加强免疫.

  7. Detection of HBV DNA by PCR on HBsAg negative blood donors%HBV DNA PCR检测在HBsAg阴性献血人群中的应用

    Institute of Scientific and Technical Information of China (English)

    陈筱华; 林碧; 刘保林; 孔令光

    2008-01-01

    Objective To define the application value of HBV DNA detection on HBsAg-negative blood donors and assess the necessity for nucleic acid detection.Methods Real-time PCR was used to detect HBV DNA on HBsAg negative blood donors.Pools of eight donor samples were used for NAT testing.Viruses were concentrated by centrifugation and the viral DNA extraction was performed using magnetic beads.If HBV DNA wag positive,serological indicators including HBsAg,anti-HBs,HBeAg, anti-Hbe,total anti-HBc was further detected.Results The HBV DNA detection limit was 25 U/ml.There were four HBV DNA positive cases among 23 225 specimens.and the detection rate was 0.17‰ The further serological examination showed anti-Hbe(+),anti-HBc(+) in the two cases and anti-HBc(+) in one case and anti-Hbs(+),anti-HBc(+)in 1 case.The viral load can range form 50 to 200 U/ml. Conclusions The results indicate that there is false negative possibility in blood screening by ELISA.It is necessary to employ anti-Hbe screening or NAT to blood donors screening.%目的 探讨HBsAg阴性献血者HBV DNA榆测的应用价值,评估核酸检测的必要性.方法 采用PCR检测HBsAg阴性献血者HBV DNA.采用8人份混合血样测定,超离心浓缩病毒,磁珠法提取病毒核酸.如HBV DNA为阳性,则进一步检测乙型肝炎病毒血清标志物5项.结果 HBVDNA检测限量为25 U/ml,23 225份标本中有4份为HBV DNA阳性,检出率为0.17‰.进一步检测其他HBV感染的血清学指标,发现这4份标本中有2份为抗HBe和抗HBc阳性,1份为抗HBc阳性,1份为抗HBs、抗HBc阳性.对HBV DNA的定量测定表明,其含量在50~200 U/ml.结论 现行的2次酶联免疫技术的血液筛查存在HBV漏检,有必要在现有的血液筛查模式中增加抗HBc检测,或增加病毒核酸筛查.

  8. Quantitative HBsAg and HBeAg predict hepatitis B seroconversion after initiation of HAART in HIV-HBV coinfected individuals.

    Directory of Open Access Journals (Sweden)

    Gail V Matthews

    Full Text Available OBJECTIVE: Anti-HBe seroconversion and HBsAg loss are important therapeutic endpoints in patients with hepatitis B virus (HBV infection. Quantitative measures of hepatitis B surface antigen (qHBsAg and e antigen (qHBeAg have been identified as potentially useful indicators of therapeutic response in HBV monoinfection. The aim of this study was to examine serological change including quantitative biomarkers in HIV-HBV coinfected patients initiating HBV active antiretroviral therapy (ART. METHODS: HIV-HBV coinfected individuals from Thailand were followed for up to 168 weeks post ART. Rates and associations of qualitative serological change were determined. Longitudinal changes in qHBsAg and qHBeAg were measured and their utility as predictors of response examined. RESULTS: Forty seven patients were included of whom 27 (57% were HBeAg positive at baseline. Median CD4 count was 48 cells/mm(3. Over a median follow-up of 108 weeks 48% (13/27 lost HBeAg, 12/27 (44% achieved anti-HBe seroconversion and 13% (6/47 HBsAg loss. Anti-HBe seroconversion was associated with higher baseline ALT (p = 0.034, lower qHBsAg (p = 0.015, lower qHBeAg (p = 0.031 and greater HBV DNA decline to week 24 (p = 0.045. Sensitivity and specificity for qHBsAg and qHBeAg decline of >0.5 log at week 12 and >1.0 log at week 24 were high for both anti-HBe seroconversion and HBsAg loss. CONCLUSIONS: Rates of serological change in these HIV-HBV coinfected individuals with advanced immunodeficiency initiating HBV-active ART were high. Baseline and on treatment factors were identified that were associated with a greater likelihood of subsequent anti-HBe seroconversion, including both quantitative HBsAg and HBeAg, suggesting these biomarkers may have utility in this clinical setting.

  9. Prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre del IMSS, Orizaba, Veracruz, México

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    Ramos-Ligonio Angel

    2006-01-01

    Full Text Available OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70 y un extracto crudo de epimastigotes. Las muestras fueron obtenidas entre los meses de octubre de 2001 a enero de 2002. RESULTADOS: Los 420 donadores de sangre analizados fueron seronegativos para HBV, HCV, BrA, VDRL y HIV. Después del tamizaje de los 420 donadores, se identificaron dos individuos seropositivos por las pruebas de ELISA, Western blot e IFI, con una seroprevalencia de 0.48%. CONCLUSIONES: En este estudio se muestran evidencias de seropositividad para T. cruzi en donadores de sangre del HGRO, lo que sugiere la existencia de riesgo de contaminación por transfusión sanguínea. Por tal motivo, es necesario aplicar programas para el tamizaje serológico a través de técnicas inmunológicas con alta sensibilidad y especificidad.

  10. Prevalencia de anticuerpos para Trypanosoma cruzi en caninos de dos municipios endémicos de Boyacá

    Directory of Open Access Journals (Sweden)

    Diego Manrique Abril

    2012-04-01

    Full Text Available Objetivo. evaluar la prevalencia de anticuerpos anti Trypanosoma cruzi (T. cruzi en una muestra de caninos domésticos residentes en dos municipios endémicos. Materiales y métodos. Se tomaron muestras séricas de 20 caninos procedentes de hogares donde residen mujeres gestantes seropositivas y 40 perros habitantes de hogares de mujeres gestantes seronegativas en Miraflores y Moniquira, Boyacá. El análisis se realizó mediante prueba diagnóstica rápida dipstick de InBios. Resultados. Se encontró prevalencia del 16.7% en Moniquirá y del 13.3% Miraflores respectivamente con una prevalencia general del 15% en los dos municipios. Se halló riesgo 3 veces mayor de que ocurra la infección en caninos, en los hogares donde residen gestantes seropositivas; además la infestación por pulgas y garrapatas en el animal, hábitat cercano a la vivienda, se relacionan con mayor seropositividad en el canino. Conclusiones. La raza, el sexo, la presencia de aves en la casa y al examen clínico general son considerados factores pronósticos en en la infección por Trypanosoma cruzi en caninos. Como factores protectores se identificó la desparasitación y vacunación de los animales.

  11. ECi/ECiQ和AxSYM全自动免疫系统的性能比较%Laboratory evaluation of the Eci/ECiQ and AxSYM automatic immunoassay system for detection of HBsAg and anti-hepatitis C virus

    Institute of Scientific and Technical Information of China (English)

    曾争; 于敏; 张乃临; 公维波

    2007-01-01

    目的:比较ECi/ECiQ和AxSYM系统对样品检测的重复性、气泡检出率及线性范围,讨论其对临床检测结果的影响.方法:HBsAg/抗-HCV强阳性样品各20份,正常人血清标本中分别加入一定比例的上述样品至S/Co比值在阈值(Cut-Off)并重复检测40次作重复性实验;气泡检出试验是在样品中加入50μL气体并重复检测20次;正常人血清连续系列稀释HBsAg强阳性标本并检测线性范围试验.结果:ECi/ECiQ和AxSYM系统HB sAg重复性(CV%)各为3.57%和3.87%;ECi/ECiQ和AxSYM系统抗-HCV各为5.05%和17.01%.ECi/ECiQ和AxSYM系统HBsAg气泡检出率各为100%和55%;ECi/ECiQ和AxSYM系统抗-HCV各为35%和15%.ECi/ECiQ系统和AxSYM系统HBsAg均未出现假阴性;ECi/ECiQ系统抗-HCV未出现假阴性,而AxSYM系统为70.6%.ECi/ECQ在稀释1∶40000时仍阳性,其线性相关系数为0.9957,而AxSYM系统在稀释1∶10000时阳性,其线性相关系数为0.9384.结论:ECi/ECiQ和AxSYM两系统均适用于临床检测,但ECi/ECiQ系统对整个检测流程质量控制要求高,而AxSYM系统对样品的质量要求相对较低,在低值标本检测中容易造成假阴性,ECi/ECiQ系统的敏感性和重复性比AxSYM系统好.

  12. Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants

    DEFF Research Database (Denmark)

    Schirmbeck, Reinhold; Dikopoulos, Nektarios; Kwissa, Marcin;

    2003-01-01

    Processing exogenous hepatitis B surface antigen (HBsAg) of the hepatitis B virus (HBV) generates the K(b)-binding S(208-215) epitope 1; processing endogenous HBsAg generates the K(b)-binding S(190-197) epitope 2. Cross-reactive CD8(+) T cell responses were primed to epitope 1 but not epitope 2...... HBs-tg mice showed reduced antigenemia. Hence, vaccination with natural HBsAg variants from different HBV sero/genotypes can prime cross-reactive, specific CD8(+) T cell immunity that breaks tolerance to HBsAg....

  13. Madopar HBS in Parkinson patients with nocturnal akinesia.

    Science.gov (United States)

    Jansen, E N; Meerwaldt, J D

    1988-01-01

    Madopar Hydrodynamically Balanced System (HBS), a new sustained-release levodopa preparation, was used to control severe nightly disabilities in 15 outpatients suffering from Parkinson's disease in an advanced state and with long-term levodopa therapy. This medication was given ante noctem in addition to an otherwise unchanged daily regimen of levodopa administration. In 13 patients a considerable diminution in nocturnal akinesia and in the frequency of waking up was reached with a mean dosage of 308 mg of Madopar HBS. Early morning akinesia was only slightly alleviated in four patients. The nocturnal off-period pain disappeared in one patient. Adverse effects consisted of nocturnal dyskinesia in two patients and early morning dystonia in another two patients. The regular use of sleeping pills was clearly reduced after Madopar HBS therapy.

  14. Papel de los anticuerpos en la protección contra micobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Nesty Olivares

    2006-12-01

    Full Text Available Convencionalmente se asume que la defensa del hospedero contra Mycobacterium tuberculosis se basa en los mecanismos de inmunidad celular exclusivamente y se descarta el papel de los anticuerpos en la protección. En este trabajo se analizan evidencias recientes que retan este dogma y sugieren la importancia de considerar la manipulación de la respuesta inmune humoral como una alternativa en la investigación de vacunas contra la tuberculosis.

  15. Anticuerpos anticardiolipina en pacientes con hipertensión arterial esencial

    OpenAIRE

    Diumenjo, M. S.; Testasecca, E.; Testasecca, A.; Maneschi, E.

    2014-01-01

    Los anticuerpos antifosfolípidos pueden activar el endotelio, modificar su función, alterar la regulación del tono vascular, producir daño renal, y así participar en la patogenia de la hipertensión arterial esencial. Antiphospholipid antibodies can activate the endothelium, alter their function, alter the regulation of vascular tone, cause kidney damage, and thus participate in the pathogenesis of essential hypertension. Fil: Diumenjo, M. S.. Universidad Nacional de Cuyo...

  16. Quantitative anti- HBs test application in observation of HBV vaccine inoculating effect and determination of booster schedule%抗-HBs定量检测在观察乙肝疫苗接种效果和确定复种方案方面的价值

    Institute of Scientific and Technical Information of China (English)

    唐跃华; 谢健敏; 刘建辉

    2004-01-01

    目的探讨定量检测抗-HBs在乙型肝炎疫苗接种效果观察和选择复种方案上的必要性.方法采用AUSAB试剂在AXYMS全自动免疫分析仪上定量检测2年前全程免疫或加强免疫过乙型肝炎疫苗而酶联免疫吸附(ELISA)定性检测抗-HBs(-)的174份血清标本的抗-HBs含量.结果174份ELISA定性检测抗-HBs(-)的标本中有45份(25.86%)抗-HBs>10mIU/mL,达到预防感染的最低水平.结论ELISA定性检测抗-HBs不能准确评价乙型肝炎疫苗接种效果和被接种者的应答状态.评价乙型肝炎疫苗接种效果尤其是确定无(弱)应答者和选择复种方案应该依据抗-HBs的定量结果.

  17. Immune Responses to a Dicistronic Plasmid Expressing HBsAg of Hepatitis B Virus and Interferon-γ

    Institute of Scientific and Technical Information of China (English)

    WANG Chun-yi; QI Feng-chun; WU Xiao-juan; ZHAO Da-peng; LENG Mei; SHENG Jun

    2007-01-01

    DNA vaccines encoding a viral protein have been shown to induce antiviral immune responses and provide protection against subsequent viral challenge. The present article deals with the efficacy of a DNA vaccine greatly improved by the simultaneous expression of HBsAg and interferon-γ gene. We constructed a dual expression vector pHIN encoding the HBsAg of Hepatitis B virus and murine IFN-γ which are connected with Internal Ribosome Entry Site(IRES). Mice inmunized with this dual expression DNA vaccine exhibited the enhancement of cellular immune response and increased the production of anti-HBV surface antibody, compared with the mice of single gene expression control. Taken together, these results demonstrate that the application of a cytokine gene in a DNA vaccine formulation as an adjuvant can improve its immunigenicity.

  18. UltramicroELISA indirecto para la deteccion de anticuerpos totales a citomegalovirus en suero humano Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples

    Directory of Open Access Journals (Sweden)

    Jose Laferte

    1992-02-01

    Full Text Available Se normalizó un ultramicroELISA indirecto para la detección de anticuerpos a Citomegalovirus (CMV humano (UMELISA CMV. Se determinó la concentración óptima de antígeno en 30 ug/ml, la dilución de los sueros fue de 1:40 y la dilución de trabajo del conjugado fue de 1:1500. El UMELISA CMV fue comparado con las técnicas de aglutinación de latex para anticuerpos anti-CMV (Dupont de Neumors y la inmunofluorescencia indirecta (EFT. Los resultados mostraron un alto grado de concordancia y elevada copositividad y conegatividad del UMELISA con respecto a estos dos ensayos. El método es válido para el pesquisaje de anticuerpos en banco de sangre asi como para el diagnóstico de la infección mediante sueros pareados.We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV using human serum samples (UMELISA CMV. The optimal concentration of coating antigen (30 ug/ml, serum dilution (1:40 and anti-human conjugate working dilution (1:1500, were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours and with an indiret immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.

  19. OBTENCIÓN DE ANTICUERPOS ESPECÍFICOS PARA LA DETECCIÓN DEL Tamarillo leaf malformation virus (TALMV EN TOMATE DE ÁRBOL

    Directory of Open Access Journals (Sweden)

    YULIANA GALLO GARCÍA

    2011-01-01

    Full Text Available En Colombia el rendimiento del cultivo de tomate de árbol se ha visto seriamente afectado por la expansión de una enfermedad conocida como virosis de tomate de árbol. Esta patología se registró inicialmente en 1991 en el norte de Antioquia y su expansión ha alcanzado todas las regiones cultivadoras de este frutal en el país. Trabajos recientes han detectado la presencia de por lo menos dos especies del género Potyvirus (Potyviridae asociadas a esta enfermedad en los cultivos de tomate de árbol de Antioquia: Potato virus Y (PVY y Tamarillo leaf malformation virus (TaLMV, especie propuesta. Con el fin de reducir la diseminación de estos patógenos virales en el país, es necesario contar con herramientas de diagnóstico que permitan la certificación del material de siembra y la detección tem- prana en plantas asintomáticas. En este trabajo se obtuvieron anticuerpos policlonales específicos para la detección del virus TaLMV utilizando una región antigénica de 15 re- siduos de la cápside viral. La sensibilidad y especificidad de los anticuerpos anti-TaLMV fue evaluada mediante pruebas de ELISA y dot-blot utilizando proteína recombinante y péptidos sintéticos como controles. La utilidad de estos anticuerpos fue validada a partir de una prueba piloto de detección de TaLMV en muestras de plantas de tomate de árbol con y sin síntomas de virosis obtenidas en el oriente antioqueño. Los resultados serolgicos fueron comparados con los niveles de detección que ofrece la técnica de RT-PCR con cebadores específicos para la cápside viral de TaLMV.

  20. The investigation of immune escape mutant HBsAg in the quinquagenarian carriers in Wuhan area by ELISA differential analysis%采用ELISA差减分析法探讨武汉地区中老年患者IEM HBsAg的携带状态

    Institute of Scientific and Technical Information of China (English)

    殷波涛; 李方和; 张永东; 张春燕; 陈妍; 周明权

    2013-01-01

    目的 探讨武汉地区中老年患者血清免疫逃逸变异表面抗原(IEM HBsAg)携带状态.方法 以2种不同抗HBs mAb包被,采用酶联免疫吸附法(ELISA)对1 641例中老年既往HBV感染者的血清标本进行检测,并以中和实验及广谱IEM HBsAg试剂对单一阳性者血清HBsAg检测的特异度进行验证.结果 所建立的G6-ELISA与E2-ELISA具有大致相同的稳定性、特异度与灵敏度,对该组血清检测的阳性率分别为29.37%、29.01%.6例G6-ELISA单一阳性血清中,抗HBs、抗HBe、抗HBc、HBeAg及 HBV DNA阳性例数分别为3、1、6、1、2例,抗HBs中和实验阳性率为100.00%,平均中和率81.14%.雅培化学发光试剂复核此6例均为阳性结果.结论 结合文献与该课题组既往研究分析,该组中老年患者中存在少量呈优势表达的IEM HBV感染者.%Objective To investigate the situation of the immune escape mutant HBsAg in the quinquagenarian carriers in Wuhan area by using ELISA differential analysis. Methods Two different anti-HBs mAbs were used as coated antibodies to detect HBsAg in 1 641 quinquagenarian HBV carriers under the same reagents and operation condition. Neutralization reactions and broad spectrum immune escape mutant HBsAg reagents were used to verify the specificity of the HBsAg positive reaction in single regent above-mentioned. Results The two reagents respectively named G6-ELISA and E2-ELISA showed similar in the stability,specificity and sensitivity. The positive rates of the two reagents in detecting the quinquagenarian serum HBV carriers were 29. 37% and 29. 01 % respectively. In the 6 positive samples which were detected by single G6-ELISA,the positive samples of anti-HBs, anti-HBe,anti-HBc, HBeAg and HBV DNA were 3,1,6,1 and 2 respectively. The positive rate in anti-HBs neutralization test was 100. 00%,and mean neutralization rate was 81. 14%. 6 samples all showed positive reactions in Abbott chemoluminescence regent. Conclusion Combining the

  1. Posintro™-HBsAg, a modified ISCOM including HBsAg, induces strong cellular and humoral responses

    DEFF Research Database (Denmark)

    Schiött, Asa; Larsson, Kristina; Manniche, Søren;

    2011-01-01

    To improve the hepatitis B vaccines on the market new adjuvant systems have to substitute aluminium. In this study the hepatitis B surface antigen (HBsAg) was incorporated into a novel adjuvant system, the Posintro™, a modification of the traditional immune stimulatory complexes (ISCOMs). This ne...

  2. Application of a Newly Developed High-Sensitivity HBsAg Chemiluminescent Enzyme Immunoassay for Hepatitis B Patients with HBsAg Seroclearance

    OpenAIRE

    2013-01-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstr...

  3. Prevalencia de anticuerpos neutralizantes contra los serotipos del virus dengue en universitarios de Tabasco, México Prevalence of neutralizing antibodies to dengue virus serotypes in university students from Tabasco, Mexico

    Directory of Open Access Journals (Sweden)

    Gilma Guadalupe Sánchez-Burgos

    2008-10-01

    Full Text Available OBJETIVO: Determinar la seroprevalencia de anticuerpos neutralizantes de los serotipos del virus dengue en estudiantes universitarios de Tabasco, México, durante los meses de septiembre a noviembre del año 2005. MATERIAL Y MÉTODOS: Se determinó la presencia de IgG contra el virus en el suero de estudiantes que acudieron al centro clínico de la universidad; en los sueros positivos se determinaron los anticuerpos neutralizantes mediante el ensayo de reducción de placa lítica. RESULTADOS: La prevalencia de IgG contra el dengue fue de 9.1%; de esta proporción, los anticuerpos neutralizantes fueron DENV-1 (20%, DENV-2 (100%, DENV-3 (4% y DENV-4 (68%. CONCLUSIONES: Este estudio muestra que el serotipo transmitido con mayor frecuencia en el estado de Tabasco es el DENV-2, aunque no ha sido el aislado con más frecuencia. La elevada prevalencia de anticuerpos neutralizantes contra el DENV-4, al parecer de reacción cruzada, podría explicar la baja circulación de este serotipo en Tabasco.OBJECTIVE: Determine the seroprevalence of neutralizing antibodies to dengue virus in students from the state university of Tabasco, Mexico. MATERIAL AND METHODS: A transversal study was conducted of serum collected from students between September and November, 2005. The sera were screened for anti-dengue IgG and those that had evidence of dengue antibodies were analyzed by a plaque reduction neutralization test. RESULTS: Prevalence of anti-dengue IgG was 9.1%. The frequency of neutralizing antibodies was 100% for DENV-2, 68% for DENV-4, 20% for DENV-1, and 4 % for DENV-3. CONCLUSIONS: We found that in this population, DENV-2 circulates more than DENV-3 despite the fact that DENV-3 is more frequently isolated. Unexpectedly, neutralizing antibodies against DENV-4 were frequently found even though this serotype is almost extinct; thus, it is probable that cross-immunity could suppress DEN-4 transmission, as has been suggested.

  4. Obtención de anticuerpos policlonales IgY antiparvovirus canino y evaluación en un sistema de aglutinación con látex

    Directory of Open Access Journals (Sweden)

    Jannet González-Figueredo

    2015-12-01

    Full Text Available La parvovirosis canina es una de las principales infecciones que provoca gastroenteritis, fundamentalmente en cachorros, con altos índices de morbiletalidad. Los diagnosticadores más utilizados se basan en la detección de partículas virales excretadas durante la fase aguda de la enfermedad. Algunos requieren equipos especializados, lo que aumenta los costos y el tiempo de diagnóstico. Por esto, la simplificación de estos métodos con alta sensibilidad y especificidad es prioritaria. En Cuba solo se logra un diagnóstico presuntivo sin la completa confirmación de la enfermedad, principalmente por la escasez o ausencia de un medio diagnóstico en toda la red de consultorios y clínicas veterinarias, rápido, eficaz y ajustable a nuestras condiciones. Este trabajo tuvo como objetivo obtener anticuerpos policlonales IgY a partir de yema de huevo de gallina y evaluarlos mediante un sistema de látex-aglutinación, para su posible uso como terapia y principalmente en el diagnóstico. Se inmunizaron por vía intramuscular dos gallinas de raza Leghorn con una cepa atenuada de parvovirus canino (PVC tipo 2. Se aplicaron 8 inoculaciones por ave cada 15 días. Se cosecharon los huevos y se purificaron los anticuerpos por el método de sulfato de dextrana/sulfato de sodio. Se determinó el título de IgY anti-PVC por el método de Inhibición de la Hemoaglutinación (IH y con antígeno de PVC acoplado a partículas de látex de poliestireno. Por IH se obtuvo un título de IgY anti-PVC de 1:1024. Las mezclas de anticuerpos con títulos alto y medio de ambas gallinas aglutinaron con los reactivos preparados con 230 y 460 µg/mL de antígeno. La mayor intensidad de la reacción y la mejor detectabilidad correspondieron a los reactivos elaborados con los anticuerpos de mayor título.

  5. Asociación entre enfermedad cardiovascular y anticuerpos contra Chlamydia pneumoniae

    Directory of Open Access Journals (Sweden)

    García-Elorriaga Guadalupe de los A

    2002-01-01

    Full Text Available Objetivo. Precisar si existe asociación entre enfermedad cardiovascular (ECV y anticuerpos contra Chlamydia en población mexicana. Material y métodos. Estudio transversal, realizado en la Unidad de Investigación en Inmunología e Infectología -Hospital de Infectología del Centro Médico Nacional La Raza (CMNR- y en el Servicio de Cirugía Cardiovascular y Asistencia Circulatoria, del Hospital General del CMNR, Instituto Mexicano del Seguro Social (IMSS, de agosto de 1998 a abril de 2000. Se determinaron anticuerpos IgG e IgM contra C. psittaci, C. trachomatis y C. pneumoniae mediante microinmunofluorescencia, en suero de 70 pacientes con ECV hospitalizados en el CMNR, mayores de 30 años, de uno u otro sexo, y se compararon con 140 sanos, pareados por edad y sexo. Se utilizaron muestras aleatorias simples, con un tamaño poblacional de 110, una prevalencia de 50% y un nivel de confianza de 99%. Para establecer la diferencia entre las proporciones de los títulos se utilizó ji cuadrada y se calculó la razón de momios. Resultados. El 94.3% (66/70 de los pacientes presentó IgG en contra de C. pneumoniae vs 37% (52/140 de los individuos sanos (p<0.001. Conclusiones. Existe una fuerte asociación entre anticuerpos IgG hacia C. pneumoniae y ECV.

  6. OBTENCIÓN DE ANTICUERPOS POLICLONALES IgY ANTIPARVOVIRUS CANINO A PARTIR DE YEMA DE HUEVO DE GALLINA

    OpenAIRE

    Pinto, J.; Barco, M.; Afanador, M.C.; Merchán, A.M.*;; Montañez, M.F.; Andrade, F; Torres, O.

    2005-01-01

    Inmunizando un grupo de diez gallinas de postura raza Lohmann de 16 semanas de edad con parvovirus canino (CPV) cepa vacunal, se obtuvieron anticuerpos IgY policlonales contra CPV en yema de huevo.En la extracción de los anticuerpos de la yema se requirieron dos pasos, el primero para la remoción de lípidos y el segundo para la precipitación de proteínas. Para la remoción de lípidos se usó el método PBS:Cloroformo y para la precipitación de los anticuerpos solubles (Inmunoglobulinas-IgY) se u...

  7. Asociación entre enfermedad cardiovascular y anticuerpos contra Chlamydia pneumoniae

    OpenAIRE

    García-Elorriaga Guadalupe de los A; Calderón-Abbo Moisés; González-Bonilla César R

    2002-01-01

    Objetivo. Precisar si existe asociación entre enfermedad cardiovascular (ECV) y anticuerpos contra Chlamydia en población mexicana. Material y métodos. Estudio transversal, realizado en la Unidad de Investigación en Inmunología e Infectología -Hospital de Infectología del Centro Médico Nacional La Raza (CMNR)- y en el Servicio de Cirugía Cardiovascular y Asistencia Circulatoria, del Hospital General del CMNR, Instituto Mexicano del Seguro Social (IMSS), de agosto de 1998 a abril de 2000. Se d...

  8. DESARROLLO Y ESTANDARIZACION DE TECNOLOGIAS PARA LA PRODUCCION DE ANTICUERPOS MONOCLONARES MODIFICADOS CON APLICACIONES EN BIOMEDICINA

    OpenAIRE

    AGUILLON GUTIERREZ, JUAN CARLOS

    2004-01-01

    Las proteínas recombinantes terapéuticas, conocidas como biofármacos, han revolucionado la industria farmacéutica, esperándose una creciente demanda en los próximos años. Dentro de éstos, destacan los anticuerpos recombinantes (AcR), que pueden clasificarse en quiméricos, humanizados y completamente humanos, hallándose varios de ellos disponibles comercialmente, con aplicaciones en el tratamiento del cáncer, enfermedades autoinmunes, rechazo de transplante renal, tratamiento de cardiopatía co...

  9. Anticuerpos antifosfolípido en el lupus eritematoso sistémico

    OpenAIRE

    López Soto, Alfonso

    1990-01-01

    INTRODUCCION: El lupus eritematoso sistémico (LES) es una enfermedad autoinmune que se caracteriza por la producción de numerosos autoanticuerpos. Recientemente, se ha descrito un nuevo grupo denominado anticuerpos antifosfolípido (AAF), cuyo estudio ha despertado un gran interés por sus implicaciones clínicas y patogenéticas. Los AAF son un grupo heterogéneo de autoanticuerpos adquiridos de forma espontánea, que van dirigidos contra estructuras fosfolipídicas de las membranas celulares.La de...

  10. ¿Sabía usted? Anticuerpos antifosfolípidos

    Directory of Open Access Journals (Sweden)

    Javier Molina

    1992-02-01

    Full Text Available

    Los anticuerpos antifosfolípidos comprenden varias poblaciones de inmunoglobulinas, que pueden ocasionar falsos positivos biológicos con el VDRL, interferir con el complejo activador de la protrombina (anticoagulante lúpico o reaccionar con elfosfolípido cardiolipina en una prueba de enzimoinmunoanálisis (EIA.

  11. Cubeta para la incubación y lavado de anticuerpos

    OpenAIRE

    Nogal París, María Luisa; Revilla Novella, Yolanda

    2008-01-01

    Cubeta para la incubación y lavado de anticuerpos. La cubeta comprende un cuerpo (1) lavable, inerte y no poroso, que está dotado de unos pocillos (2) ubicados en su interior, cuyo número y tamaño puede variar en función del requerimiento experimental. En dichos pocillos (2) se introducen preferiblemente fragmentos o tiras (3) de nitrocelulosa, que contienen proteínas que han sido previamente transferidas desde un gel de poliacrilamida utilizando un campo eléctrico. Es...

  12. [Diagnostic value of the quantitative HBsAg determination in hepatitis B infections].

    Science.gov (United States)

    van Helden, J; Weiskirchen, R

    2016-04-01

    Introduction of systematic hepatitis B vaccination has lead to a strong decrease of new infections, but there are still a high numbers of chronically infected persons suffering on long-term complications. Using quantitative assays for the determination of HbsAg (qHBsAg) has improved our understanding of chronic hepatitis B (CHB). The concentrations of HBsAg are strongly varying through the different stages of infection. The quantitative determination of HBsAg does not only yield in additional information to the infection activity, but also provides data for an improved follow up independent from the virus load. As to the prediction of disease progression, low-viremic carriers with high HbsAg levels have been shown to be at higher risk of HBeAg negative hepatitis, cirrhosis and hepatocellular carcinoma (HCC). Although, quantitative HBsAg determination has been widely used in CHB patients receiving pegylated interferon therapy, the HbsAg decline is slow compared to HBV-DNA levels during nucleos(t)ide analogue (NUC) therapy. However a rapid HbsAg decline during NUC therapy may identify patients who will finally clear HbsAg. A 6- to 12-monthly assessment of HbsAg level could be considered during NUC therapy. Taking these lines of evidence together, qHBsAg can complement HBV-DNA levels to optimize the management of CHB patients.

  13. Frecuencia de anticuerpos contra el virus C de la hepatitis en pacientes con cirrosis hepática en Yucatán, México

    Directory of Open Access Journals (Sweden)

    Góngora-Biachi Renán A

    2003-01-01

    Full Text Available OBJETIVO: En este estudio reportamos la prevalencia de anticuerpos contra el virus C de la hepatitis (Ac-VCH en un grupo de pacientes con cirrosis hepática (CH. MATERIAL Y MÉTODOS: Se hizo un estudio prospectivo, transversal y descriptivo, de marzo de 1998 a mayo de 1999. Se estudiaron a 153 pacientes (117 (76% hombres y 36 (24% mujeres con diagnóstico de CH, que eran atendidos en el Hospital General Agustín O' Horan y en el Centro de Investigaciones Regionales Doctor Hideyo Noguchi, en la ciudad de Mérida, Yucatán, México. Se aplicó un cuestionario con datos clínico-epidemiológicos y se determinó la presencia de Ac-VCH (ELISA de 2ª generación y RIBA-2 para confirmar el diagnóstico a cada paciente. Se determinó también el antígeno de superficie de la hepatitis B (AgsHB y anticuerpos contra el antígeno central de la hepatitis B (Anti-HBc mediante el método de ELISA. La presencia de Ac-VCH fue relacionada con las variables epidemiológicas de los sujetos. La prevalencia de anti-HCV y la frecuencia de características se compararon entre los pacientes positivos y negativos con las pruebas de c² y exacta de Fisher. RESULTADOS: El 32% de los pacientes con CH (35/117 (30% hombres y 14/36 (39% mujeres fueron positivos para los Ac-VCH. El alcoholismo estuvo presente en todos los hombres serorreactivos y en ninguna de las mujeres positivas (p< 0.001. Ninguna de las variables epidemiológicas analizadas estuvo asociada con la seropositividad. Los anti-HBc se encontraron en 16% de los pacientes positivos para Ac-VCH y en 12% de los seronegativos (p=0.69. CONCLUSIONES: La prevalencia encontrada fue mayor a los reportes previos realizados en población general de la Península de Yucatán (1.3%. La alta prevalencia de Ac-VCH en este grupo de pacientes sugiere que la CH está más frecuentemente asociada con el VCH en Yucatán, México, que con el virus B de la hepatitis. El alcoholismo probablemente actúa como un cofactor en el

  14. Inmunodeficiencias primarias por déficit de anticuerpos en adultos

    Directory of Open Access Journals (Sweden)

    María Mercedes Bello Rodríguez

    2014-10-01

    Full Text Available Se realizó una investigación sobre los pacientes con inmunodeficiencias primarias por déficit de anticuerpos, atendidos en el servicio de inmunología del Hospital General “Dr. Ernesto Guevara” de Las Tunas, con el objetivo de caracterizar las inmunodeficiencias por déficit de anticuerpos en pacientes adultos, atendiendo al tipo de inmunoglobulina afectada, el sexo y la edad, así como las principales manifestaciones clínicas. Se evaluaron un total de diez pacientes tratados por esta causa entre los años 2005 y 2013. Para la evaluación de los resultados se utilizó el análisis de frecuencias simple. Las inmunodeficiencias diagnosticadas se correspondieron en un 50% con el déficit de las tres clases de inmunoglobulinas. Predominó el sexo masculino en un 70% y las edades más afectadas se correspondieron con la cuarta y quinta décadas de la vida. Las infecciones respiratorias y digestivas fueron las más frecuentes (80%, tanto por gérmenes bacterianos como virales, la infección por Giardia lamblia también apareció muy frecuentemente en los pacientes estudiados

  15. Serologic tracers (HBsAg and HBsAc) of hepatitis B virus in expectant mothers of the Perinatal Maternal Institute

    CERN Document Server

    Garcia, B M

    1997-01-01

    A study on hepatitis B tracers, (HBsAg and HBsAc), was conducted in women with different months of pregnancy at the Perinatal Maternal Institute in Lima, Peru. A total of 1010 mothers were studied during the period of January to October 1996, establishing by radioimmunoassay (RIA) whether they were positive or not. The results showed a prevalence rate of 1,6 for HBsAc and 1,3 for HBsAg for every 100,000 inhabitants. The incidence rate was 0,332 for HBsAc and 0,07 for HBsAg for every 100,000 inhabitants. This means that 21 expectant mothers are HBsAc positive and 5 are HBsAg positive. According to the investigations, there were different ways of transmission; promiscuity must be highlighted, as well as age -most of the mothers who were positive were between 20 and 25 years old- and origin. Most of them were immigrants from different places and live in shantytowns, which indicates that most of them have limited economic resources and have not received any orientation on family planning.

  16. Comparison of serum HBsAg quantitation by four immunoassays, and relationships of HBsAg level with HBV replication and HBV genotypes.

    Directory of Open Access Journals (Sweden)

    Edouard Tuaillon

    Full Text Available BACKGROUND: The decline in hepatitis B virus surface antigen (HBsAg may be an early predictor of the viral efficacy of Hepatitis B virus (HBV therapy. The HBsAg levels obtained by different immunoassays now need comparing and the relationships between levels of HBsAg and HBV DNA alongside HBsAg and genotype must be evaluated. METHODOLOGY/PRINCIPAL FINDINGS: HBsAg levels were compared among 80 patients using the Abbott Architect assay, a commercial immunoassay approved for HBsAg detection and quantitation, and three other assays derived from immunoassays approved for HBsAg detection (manufactured by Diasorin, Bio-Rad and Roche. Good correlation was found between the Abbot vs. Diasorin, Bio-Rad and Roche assays with narrow 95% limits of agreement and small mean differences: -0.06 to 0.11, -0.09 log(10 IU/mL; -0.57 to 0.64, -0.04 log(10 IU/mL; -0.09 to 0.45, -0.27 log(10 IU/mL, respectively. These agreements were not affected by genotypes A or D. HBsAg was weakly correlated with HBV DNA, whatever the HBsAg assay used: Abbott, ρ = 0.36 p = 0.001, Diasorin ρ = 0.34, p = 0.002; Bio-Rad ρ = 0.37, p<0.001; or Roche ρ = 0.41, p<0.001. This relationship between levels of HBsAg and HBV DNA seemed to depend on genotypes. Whereas HBsAg (Abbott assay tended to correlate with HBV DNA for genotype A (ρ = 0.44, p = 0.02, no such correlation was significant for genotypes D (ρ = 0.29, p = 0.15. CONCLUSION/SIGNIFICANCE: The quantitation of HBsAg in routine clinical samples is comparable between the reference assay and the adapted assays with acceptable accuracy limits, low levels of variability and minimum discrepancy. While HBsAg quantitation is not affected by HBV genotype, the observed association between levels of HBsAg and HBV DNA seems genotype dependent.

  17. Studies on specific interaction of beta-2-glycoprotein I with HBsAg

    Institute of Scientific and Technical Information of China (English)

    Pu-Jun Gao; Yun-Feng Piao; Xiao-Dong Liu; Li-Ke Qu; Yang Shi; Xiao-Cong Wang; Han-Yi Yang

    2003-01-01

    AIM: To observe the binding activity of beta-2-glycoprotein I (β2GPI) to hepatitis B surface antigen (HBsAg) and the possible roles of β2GPI in hepatitis B virus (HBV) infection.METHODS: The rationale of ELISA methods and ELISAbased research method and ligand-blotting technique were used to detect the specific interaction of β2GPI with HBsAg.RESULTS: With the increase of rHBsAg, the binding of β2GPI to rHBsAg elevated, and these changes had statistic significance. When we added non- biotinlyated β2GPI, the OD value significantly decreased though they still were positively relevant to rHBsAg, suggesting non- biotinlyated β2GPI competed with biotinlyated β2GPI to saturate the binding sites on rHBsAg. Meanwhile BSA was used as negative control to substitute for rHBsAg coating the plates.The results indicated no interaction between β2GPI and BSA,suggesting the affinity of β2GPI to rHBsAg was specific. The ligand blotling indicated that β2GPI might bind to rHBsAg no matter whether it was under reduced condition or not.CONCLUSION: The binding of β2GPI to HBsAg suggests that β2GPI may be a carrier of HBV and that β2GPI may play important roles in HBV infection.

  18. Survey of both hepatitis B virus (HBsAg and hepatitis C virus (HCV-Ab coinfection among HIV positive patients

    Directory of Open Access Journals (Sweden)

    Pournia Yadollah

    2009-11-01

    Full Text Available Abstract Background HIV, HBVand HCV is major public health concerns. Because of shared routes of transmission, HIV-HCV coinfection and HIV-HBV coinfection are common. HIV-positive individuals are at risk of coinfection with HBV and HCV infections. The prevalence rates of coinfection with HBV and HCV in HIV-patients have been variable worldwide depending on the geographic regions, and the type of exposure. Aim This study aimed to examine HBV and HCV coinfection serologically and determine the shared and significant factors in the coinfection of HIV-positive patients. Methods This descriptive, cross-sectional study was carried out on 391 HIV-positive patients including 358 males and 33 females in Lorestan province, west Iran, to survey coinfection with HBsAg and anti-HCV. The retrospective demographic data of the subjects was collected and the patients' serums were analyzed by ELISA kits including HBsAg and anti-HCV. The collected data was analyzed with SPSS software (15 and Chi-square. Fisher's exact test with 5% error intervals was used to measure the correlation of variables and infection rates. Results The results of the study indicated that the prevalence of coinfection in HIV-positive patients with hepatitis viruses was 94.4% (370 in 391, out of whom 57 (14.5% cases were HBsAg positive, 282 (72% cases were anti-HCV positive, and 31 (7.9% cases were both HBsAg and anti-HCV positive. Conclusion There was a significant correlation between coinfection with HCV and HBV and/or both among HIV-positive patients depending on different variables including sex, age, occupation, marital status, exposure to risk factors.(p

  19. Analysis of Results of Anti-HBs Levels of Healthy Children in Dingxi from 2008 to 2013%定西市2008-2013年健康儿童乙肝抗体水平监测结果分析

    Institute of Scientific and Technical Information of China (English)

    陈军莲

    2015-01-01

    目的 回顾分析2008-2013年定西市健康儿童抗-HBs水平,为制定定西市乙肝控制策略提供参考依据.方法 采用随机整群抽样的方法,选择定西市2~7岁儿童632人为研究对象,采用ELISA方法检测入选儿童乙肝抗体(抗-HBs)水平.结果本次调查儿童的抗-HBs总阳性率为47.15%,不同县、区和性别儿童之间抗-HBs阳性率比较无显著性差异,不同年龄组儿童抗-HBs阳性率比较差异有统计学意义,其中2~岁组儿童阳性率最高,为72.92%,5~岁组儿童阳性率最低,为36.80%.结论 抗-HBs对儿童能够起到有效的保护作用,但是抗体效价却随着时间的延长逐渐减弱,因此,儿童群体要及时加强接种,从而有效地控制乙肝发病.

  20. OBTENCIÓN DE ANTICUERPOS POLICLONALES IgY ANTIPARVOVIRUS CANINO A PARTIR DE YEMA DE HUEVO DE GALLINA

    Directory of Open Access Journals (Sweden)

    Pinto, J.

    2005-06-01

    Full Text Available Inmunizando un grupo de diez gallinas de postura raza Lohmann de 16 semanas de edad con parvovirus canino (CPV cepa vacunal, se obtuvieron anticuerpos IgY policlonales contra CPV en yema de huevo.En la extracción de los anticuerpos de la yema se requirieron dos pasos, el primero para la remoción de lípidos y el segundo para la precipitación de proteínas. Para la remoción de lípidos se usó el método PBS:Cloroformo y para la precipitación de los anticuerpos solubles (Inmunoglobulinas-IgY se usó el método de salting-out con sulfato de amonio ((NH42SO4. La evaluación del proceso se efectuó empleando el método comercial estándar “EGGstract IgY Purification System®” de PROMEGA.La metodología empleada permitió la obtención de anticuerpos IgY policlonales contra CPV a partir de yema de huevo de gallina en concentraciones altas por mililitro de yema, con una pureza aceptable y títulos altos; los resultados fueron comparables con el método comercial.

  1. 桂林市居民乙肝疫苗接种率及乙肝表面抗体水平调查%Investigation on hepatitis B vaccination rate and HBsAb levels of residents in Guilin City

    Institute of Scientific and Technical Information of China (English)

    黄少新; 张振开; 冯厚滨

    2013-01-01

    目的 通过检测分析桂林市居民乙型肝炎(乙肝)疫苗接种率及乙肝表面抗体水平,为桂林市制定乙肝控制策略提供依据.方法 随机抽取2个社区和1个乡作为调查现场,对各调查点中随机抽选不同年龄段人群采血,运用酶链免疫吸附试验(ELISA)法检测抗-HBs,并运用SPSS 10.0对检测结果进行分析.结果 桂林市居民抗-HBs阳性率为59.83%,抗-HBs几何平均滴度(GMT)为71.79 mU/ml.抗-HBs阳性率和抗-HBs的GMT均随年龄增加而下降,各年龄组间差异有统计学意义(x2=117.911,F=92.865,均P<0.01).城区居民抗-HBs阳性率(65.12%)和抗-HBs的GMT[(93.75±7.78) mU/ml]均高于农村居民阳性率(40.59%)和GMT[(57.38 ±6.35) mU/ml].结论 桂林市居民抗-HBs阳性率和抗-HBs的GMT水平均不高,对大年龄组和农村居民加强乙肝疫苗接种很有必要.%[Objective] To analyze the hepatitis B vaccination rate and HBsAb levels of residents in Guilin City via detection,and provide evidence for developing hepatitis B control strategies.[Methods] Two communities and a town were randomly selected as survey scene.People of different age groups were randomly chosen for blood sampling,using ELISA for anti-HBs detection and SPSS 10.0 for results analysis.[Results] The positive rate of anti-HBs was 59.83%,GMT of anti-HBs was 71.79 mU/ml.The positive rate of anti-HBs and GMT of anti-HBs decreased with rising age,the differences between age groups were significant(x2 =117.911,F =92.865,all P < 0.01).The positive rate of anti-HBs (65.12%) and GMT of anti-HBs [(93.75 ± 7.78)mU/ml] of urban residents were both higher than those of rural residents (40.59% and [(57.38 ± 6.35) mU/ml)].[Conclusion]The positive rate of anti-HBs and GMT of anti-HBs are not high in Guilin City.It is necessary to strengthen hepatitis B vaccination among old age groups and rural residents.

  2. 阻断PD-1/PD-L1信号通路对负载HBsAg的树突细胞抗HBV免疫功能的影响%Effect of programmed death-1/progranuned death ligand-1 interaction on immune response of HBsAg-pulsed denddtic cells to hepatitis B virus

    Institute of Scientific and Technical Information of China (English)

    郭忠胜; 潘修成; 陈明; 杨帆; 李丽; 张言超; 李秀华

    2010-01-01

    Ag protein.HBV transgenic mice were injected i.D with PD-L1 blocking monoclonal antibodies(Abs)1 d before DC/HBsAg transfer with an interval of 3 d for 3 times,and then divided into 5 groups:DC/HBsAg+PD-L1 Abs group,DC/HBsAg group,DC group,mlgG group and phosphate buffered saline(PBS)group.Extracellular molecule and intraceilular cytokine of splenic T cells were analyzed by flow cytometry,HBsAg-specific activity of splenic cytotoxic T lymphocytes (CTLs)was detected by LDH release assay,HBsAg and HBV DNA titers in sera were monitored by ELISA and fluorescent quantitative PCR,and hepatic injury Was monitored by measuring serum ALT at 3th and 6th after immunization,respectively.SPSS 13.0 software Was used for data processing,and ANOVA(analysis of variance)as well as q test were performed to compare the measurement data among the groups.Results At 3th and 6th after immunization,PD-L1 blockade stimulated splenic CD_3~+CD_8~+T lymphocytes proliferation more efficiently than that with no PD-L1 blockade(mice with DC/HBsAg treatment alone)(F=25.22 and 39.01,P<0.01),and the secretion of IFNγ WaS hisher(F=32.35 and 36.98.P<0.01);meanwhile the differences between mice with DC/HBsAg treatment and those with DC alone were only observed at 3th(t=6.79,P=0.012).The activity of HBsAg-specific CTL and serum HBsAg levels at 6th were significantly higher in mice with anti-PD-L1 Abs treatment compared with those in other groups (F=6.12,P<0.05).Serum ALT levels in mice with anti-PD-L1 Abs treatment were the same with DC/HBsAg group at 3th,but were higher than those in other groups at both 3th and 6th(F=19.22 and 14.30,P<0.05).All groups were similar in the clearance of HBV DNA.but HBV DNA hvel decreased one log copy in one mouse with anti-PD-L1 Abs treatment.Conclusion Blocking PD-1/PD-L1 interaction can enhance specific CD_3~+CD_8~+ T lymphocyte proliferation and the capability of secreting IFNγ,which improves the inhibition of HBV in transgenic mice induced by HBsAg-pulsed DC vaccine.

  3. Anti-HBc screening in Indian blood donors: Still an unresolved issue

    Institute of Scientific and Technical Information of China (English)

    Hari Krishan Dhawan; Neelam Marwaha; Ratti Ram Sharma; Yogesh Chawla; Beenu Thakral; Karan Saluja; Sanjeev Kumar Sharma; Manish K Thakur; Ashish Jain

    2008-01-01

    AIM:To study the seroprevalence of antibody to hepatitis B core antigen (anti-HBc) in healthy blood donors negative for HBsAg and to evaluate whether anti-HBc detection could be adopted in India as a screening assay for HBV in addition to HBsAg.METHODS:A total of 1700 serum samples collected from HBsAg-negative healthy blood donors were tested for the presence of anti-HBc antibody (IgM + IgG).All samples reactive for anti-HBc antibody were then investigated for presence of anti-HBs and for liver function tests (LFTs).One hundred serum samples reactive for anti-HBc were tested for HBV DNA by PCR method.RESULTS:Out of 1700 samples tested,142 (8.4%)blood samples were found to be reactive for anti-HBc.It was significantly lower in voluntary (6.9%) as compared to replacement donors (10.4%,P=0.011).Seventytwo (50.7%) anti-HBc reactive samples were also reactive for anti-HBs with levels >10 mIU/mL and 70(49.3%) samples were non-reactive for anti-HBs,these units were labeled as anti-HBc-only.These 142 anti-HBc reactive units were also tested for liver function test.HBV DNA was detected in only 1 of 100 samples tested.CONCLUSION:Keeping in view that 8%-18% of donor population in India is anti-HBc reactive,inclusion of anti-HBc testing will lead to high discard rate.Anti-HBs as proposed previously does not seem to predict clearance of the virus.Cost effectiveness of introducing universal anti-HBc screening and discarding large number of blood units versus considering ID NAT (Individual donor nuclic acid testing) needs to be assessed.

  4. Anti-HBc screening in Indian blood donors: Still an unresolved issue

    Science.gov (United States)

    Dhawan, Hari Krishan; Marwaha, Neelam; Sharma, Ratti Ram; Chawla, Yogesh; Thakral, Beenu; Saluja, Karan; Sharma, Sanjeev Kumar; Thakur, Manish K; Jain, Ashish

    2008-01-01

    AIM: To study the seroprevalence of antibody to hepatitis B core antigen (anti-HBc) in healthy blood donors negative for HBsAg and to evaluate whether anti-HBc detection could be adopted in India as a screening assay for HBV in addition to HBsAg. METHODS: A total of 1700 serum samples collected from HBsAg-negative healthy blood donors were tested for the presence of anti-HBc antibody (IgM + IgG). All samples reactive for anti-HBc antibody were then investigated for presence of anti-HBs and for liver function tests (LFTs). One hundred serum samples reactive for anti-HBc were tested for HBV DNA by PCR method. RESULTS: Out of 1700 samples tested, 142 (8.4%) blood samples were found to be reactive for anti-HBc. It was significantly lower in voluntary (6.9%) as compared to replacement donors (10.4%, P = 0.011). Seventy-two (50.7%) anti-HBc reactive samples were also reactive for anti-HBs with levels > 10 mIU/mL and 70 (49.3%) samples were non-reactive for anti-HBs, these units were labeled as anti-HBc-only. These 142 anti-HBc reactive units were also tested for liver function test. HBV DNA was detected in only 1 of 100 samples tested. CONCLUSION: Keeping in view that 8%-18% of donor population in India is anti-HBc reactive, inclusion of anti-HBc testing will lead to high discard rate. Anti-HBs as proposed previously does not seem to predict clearance of the virus. Cost effectiveness of introducing universal anti-HBc screening and discarding large number of blood units versus considering ID NAT (Individual donor nuclic acid testing) needs to be assessed. PMID:18785287

  5. 麻鸭血替代人血测定HBsAg-ELISA法在校内实训中的应用研究%The Research of The Testing Way of HbsAg - ELISA with Ma Duck Blood Replacing Human Blood in the training Courses in Our College

    Institute of Scientific and Technical Information of China (English)

    吴展奎; 宋爱萍

    2012-01-01

    为了避免实验室生物安全事故(学生感染和实验室污染)的发生,探讨采用麻鸭血替代人血用酶联免疫吸附试验(ELISA法)作乙肝病毒表面抗原(HBsAg)检测的可行性。方法:收集黔东南地区100份麻鸭血标本,采用ELISA法检测HBsAg。结果 100份麻鸭血清标本HBsAg检测结果均为阴性。结论:我国多地报道麻鸭血携带鸭乙肝病毒(DHBV),但通过检测,我地区麻鸭血HBsAg为阴性,在校内实训课中可作为标本应用。%purpose In order to avoid the accidents (infection on the students and pollution to the lab) in the biological lab, we have explored the possibility of testing Hepatitis B virsu Surface Anti C, en(HbsAg) in the way of Enzyme Linked Immunosorbent Assay ( ELISA), with the Ma Duck blood replacing the human blood. Methodology Collect the blood from 100 Ma ducks in the area of Qiandongnan as the samples, then test HBsAg using the way of ELISA. Results All the blood samples from the 100 Ma ducks show the test resuls are negative. Conclusion As reported, Ma duck blood carries the Duck Hepatitis B Virus (DHBV) in most areas of China, however, Ya duck blood in the area of Qiandongnan is negative in the test, so it can be appiled for samples in the training courses.

  6. Anticuerpos séricos contra la enfermedad de Newcastle e Influenza Aviar en aves rapaces de Chile.

    Directory of Open Access Journals (Sweden)

    Daniel González-Acuña

    2012-12-01

    Full Text Available Objetivo. Detectar la presencia de anticuerpos séricos sanguíneos contra los virus de la Enfermedad de Newcastle (ENC e Influenza aviar (IA, para comprender la contribución de las aves silvestres en la transmisión de estos virus en Chile. Materiales y métodos. Se analizaron 63 aves pertenecientes a los órdenes Falconiformes y Strigiformes desde centros de rehabilitación de aves de las zonas central y sur de Chile. Se realizaron las pruebas de inhibición de la hemoaglutinación (IHA para detectar anticuerpos contra el virus ENC e inmunodifusión en gel agar (IDGA y ELISA para IA. Resultados. Se detectaron 14 aves positivas (22.2% para anticuerpos séricos contra el virus de la ENC. En cambio, no se registraron anticuerpos séricos sanguíneos para el virus de la IA. Conclusiones. La presencia de aves rapaces positivas en los centros de rescate a los anticuerpos séricos contra el virus de la ENC puede ser explicada por el consumo de carne de pollos que han sido vacunados contra ENC o consumo de aves que han adquirido directamente el virus vacunal a través de los distintos procedimientos de administración (aerosoles, bebederos de la vacuna o por el ingreso a los centros de rescate de aves rapaces migratorias, las que podrían facilitar la diseminación de la infección desde los países de origen, hecho que debe ser investigado.

  7. Construction and characterization of calreticulin-HBsAg fusion gene recombinant adenovirus expression vector

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine.METHODS: CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease diges-tion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5′ end. Adenoviral expression vector containing CRT-HBsAg fusion gen...

  8. HBsAg阴性母亲及其婴儿乙肝疫苗接种情况和免疫效果研究%The Status of Hepatitis B Vaccine Immunization and the Immune Effects of HBsAg Negative Mothers and Their Infants

    Institute of Scientific and Technical Information of China (English)

    张维璐; 郭振军; 张磊; 李晶华; 徐剑秋; 张景霞; 邵中军

    2013-01-01

    Objective: To study the status of hepatitis B vaccine immunization and the anti-HBs titers of HBsAg negative mothers and their infants so as to make contributions to their immunization strategies of hepatitis B vaccine. Methods: A cross-sectional study was conducted with a standardized questionnaire in 227 pairs of mothers and infants (month age were 8~24 months) from May 2010 to October 2010. Their peripheral blood was withdrawn for anti-HBs detection. Results: 45.4% of mothers were anti-HBs-positive, and the GMT of the titers was 12.88 mIU/mL (95% CI: 8.91-18.19). The rates of the first dose, the second dose and the third dose of hepatitis B vaccine vaccinated on time were 95.2%, 93.8% and 85.9% respectively. 77.1% of infants were anti-HBs-positive, and the GMT of the titers was 37.15 mIU/mL (95% CI: 28.18-48.98). Conclusions: The rate was high for the first dose of the infant's hepatitis B vaccine vaccinated on time. And the rates of all the 3-dose immunization which were vaccinated on time still need to be improved. The immunity of hepatitis B vaccine and the detection of HBV markers for HBsAg negative women of childbearing age should be paid more attention, which can improve the level of immunity against HBV of this population.%目的:了解乙肝表面抗原阴性(HBsAg阴性)母亲及其婴儿乙肝疫苗接种情况及抗-HBs滴度水平,从而为今后针对该特殊人群进行更好的乙肝疫苗免疫策略提供依据.方法:2010年5月~2010年10月,对陕西省227对HBsAg阴性母亲及其婴幼儿(月龄为8~24月)进行流行病学调查并采集血液标本,对母婴血清抗-HBs进行定性及定量检测.结果:母亲乙肝表面抗体(抗-HBs)阳性率为45.4%,抗-HBs平均滴度为12.88 mIU/mL(95% CI:8.91-18.19).婴儿乙肝疫苗首针、第二针和第三针的及时接种率分别为95.2%,93.8%和85.9%.婴儿抗-HBs阳性率为77.1%,抗-HBs平均滴度为37.15 mIU/mL(95% CI:28.18-48.98).结论:婴儿乙肝疫苗首针及

  9. Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells

    Institute of Scientific and Technical Information of China (English)

    Shan Qin; Hong Tang; Lian-San Zhao; Fang He; Yong Lin; Li Liu; Xiao-Mei He

    2003-01-01

    AIM: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monoclone SP2/0 cell strains that are capable of expressing S or S2S proteins stably.METHODS: Segments of S, preS2-S, preS1-preS2-S genes of Hepatitis B virus were amplified by routine PCR and preS1S fragment was amplified by Over-Lap Extension PCR. The amplified segments were cleaved with restricted endonuclease Hind Ⅲ/Not Ⅰ followed by ligation with pRc/CMV, or BamHI/EcoR Ⅰ followed by ligation with pSG5UTPL/Flag. After the plasmid vectors were cleaved with the correspond enzymes, the amplified segments were inserted into pRc/CMV or pSGSUTPL/Flag plasmid vectors with T4DNA ligase. KOZAK sequence was added before the initial ATG code of each fragment using specific primer. The inserted segments in the recombinant plasmids were sequenced after subcloning. BALB/c mice myeloma cells (SP2/0 cell line) were transfected with the recombinant plasmids. The expressions of the different recombinants were compared by Western-blot, using a monoclonal anti-HBs antibody as the primary antibody and peroxidase-labeled multi-linker as the secondary. Stable SP2/0-pRc/CMV-S or SP2/0- pRc/CMV-MS clones were established through clone screening with G418.RESULTS: Fragments with anticipated size were harvested after PCR. After recombination and screening, the sequences of the inserted segments in the recombinants were confirmed to be S, preS2S, preSl-preS2S and preSlS encoding genes,determined by sequencing. The results of Western-blot hybridization were positive for the anticipated proteins.Among them, pRc/CMV-S or pRc/CMV-MS demonstrated the highest expressing their respective antigen.CONCLUSION: Eight recombinant plasmids expressing S,M, L or preSlS proteins are obtained. For hepatitis surface antigen expression in eukaryotic cells

  10. Enhanced HBsAg synthesis correlates with increased severity of fibrosis in chronic hepatitis B patients.

    Directory of Open Access Journals (Sweden)

    Mei-Zhu Hong

    Full Text Available BACKGROUND AND AIMS: Little is known about whether low serum HBsAg levels result from impaired HBsAg synthesis or a reduced number of hepatocytes caused by advanced liver fibrosis. Therefore, we investigated the capacity for HBsAg synthesis in a cross-sectional cohort of treatment-naïve chronic hepatitis B patients. METHODS: Chronic hepatitis B patients (n = 362 were enrolled; liver biopsies were performed and liver histology was scored, and serum HBsAg and HBV DNA levels were investigated. In the enrolled patients, 183 out of 362 have quantitative serum HBsAg levels. Tissue HBsAg was determined by immunohistochemistry. RESULTS: A positive correlation between serum HBsAg and HBV DNA levels was revealed in HBeAg(+ patients (r = 0.2613, p = 0.0050. In HBeAg(+ patients, serum HBsAg and severity of fibrosis were inversely correlated (p = 0.0094, whereas tissue HBsAg levels correlated positively with the stage of fibrosis (p = 0.0280. After applying the mean aminopyrine breath test as a correction factor, adjusted serum HBsAg showed a strong positive correlation with fibrosis severity in HBeAg(+ patients (r = 0.5655, p<0.0001. The adjusted serum HBsAg values predicted 'moderate to severe' fibrosis with nearly perfect performance in both HBeAg(+ patients (area under the curve: 0.994, 95% CI: 0.983-1.000 and HBeAg(- patients (area under the curve: 1.000, 95% CI: 1.000-1.000. CONCLUSIONS: Although serum HBsAg levels were negatively correlated with fibrosis severity in HBeAg(+ patients, aminopyrine breath test-adjusted serum HBsAg and tissue HBsAg, two indices that are unaffected by the number of residual hepatocytes, were positively correlated with fibrosis severity. Furthermore, adjusted serum HBsAg has an accurate prediction capability.

  11. Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg

    Institute of Scientific and Technical Information of China (English)

    Yi Gao; Yina Ma; Mei Li; Tonq Chenq; Shao-Wei Li; Jun Zhang; Ning-Shao Xia

    2003-01-01

    AIM: To investigate the expression of recombinant HBsAg (rHBsAg) in transgenic cherry tomatillo in order to explore the feasibility of producing HBV oral vaccine with cherry tomatillo by animal immune tests.METHODS: The recombinant plant expression vector containing HBsAg gene was constructed. Mediated with Agrobacterium tumefaciens, HBsAg gene was transferred into cotyledons of cherry tomatillo. Transformed cherry tomatillos were obtained through hygromycin delay-selection. Integrated DNA in transgenic cherry tomatillo was confirmed by hygromycin resistance selection, Gus detection, polymerase chain reaction (PCR) and dot blotting analysis. Antigenicity of rHBsAg was examined by ELISA and the immunogenicity of rHBsAg derived from transgenic cherry tomatillo tissues was confirmed by oral feed of transformed tissues to BALB/c mice primed with commercial HBV vaccines. Specific antibody titers in mice's serum were examined by ELISA every week.RESULTS: By far, 10 positive lines of transgenic cherry tomatillos containing HBsAg gene were obtained. Among different organs of the same transgenic cherry tomatillo,level of rHBsAg expressed in leaves was the highest with the yield up to 300ng/g fresh weight. And the rHBsAg expression level in fruits was about 10 ng/g fresh weight.In animal immune tests, oral delivery with transgenic tissues to mice primed with commercial vaccine instead of naive mice resulted in significant immune response.CONCLUSION: The result of this animal immune test indicated the rHBsAg derived from transgenic cherry tomatillo possessed normal immunogenicity. This work demonstrated the feasibility to generate oral immunogenic rHBsAg in transgenic cherry tomatillo, and would provide some experimental approach for the production of low-cost oral vaccines using transgenic cherry tomatillo in large scale.

  12. Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines.

    Science.gov (United States)

    Wu, Yajun; Li, Shuai; Li, Haixia; Zhao, Chunzhi; Ma, Hao; Zhao, Xiunan; Wu, Junhua; Liu, Kunlu; Shan, Junjie; Wang, Yuxia

    2016-10-01

    Poria cocos has a long history of medicinal use in China. Polysaccharides and their derivatives in the medicine exhibit many beneficial biological activities including anticancer, anti-inflammatory, antioxidant and antiviral activities. In this study, a new polysaccharide (PCP-II) was isolated from sclerotium of Poria cocos. Its physico-chemical characters were identified and its adjuvant activity was investigated in mice co-immunized with H1N1 influenza vaccine and hepatitis B surface antigen (HBsAg). The results revealed that PCP-II has a molecular weight of 29.0kDa. It was composed of fucose, mannose, glucose and galactose in molar ration of 1.00:1.63:0.16:6.29 respectively. Pharmacological data demonstrated that PCP-II increased antigen-specific antibody levels in mice immunized with influenza vaccine. PCP-II also elicited anti-HBsAg antibodies at significantly higher titers and generated robust and durable immunity compared to mice immunized with HBsAg-alum following two administrations. PCP-II improved proliferation of splenocytes, stimulated IL-12p70 and TNF-α productions in dendritic cells and macrophages respectively. These results suggested that PCP-II-adjuvanted vaccines enhanced humoral and cellular immunity. PCP-II could be developed as an efficacious adjuvant in human and animal vaccines.

  13. Clinical Significance of HBV - DNA Detection for Coexistent Serum Models of HBsAg and HBsAb%HBV DNA 检测在血清 HBsAg 与 HBsAb 共存模式中的临床价值

    Institute of Scientific and Technical Information of China (English)

    刘伟平; 殷明刚; 阮艳秋

    2012-01-01

    目的 检测HBsAg与HBsAb共存模式血清中HBV DNA的拷贝数,探讨其在相应患者临床诊疗中的应用价值.方法 用ELISA法检测乙肝两对半,统计HBsAg与HbsAb双阳性的血清学模式分布;荧光定量PCR检测HBsAg与HBsAb共存模式标本的HBV DNA病毒载量;在96例共存模式中,按S/CO比值高低将其分为A、B、C、D 4组,计算各组的HBV DNA阳性率.结果 在所有HBsAg与HBsAb共存模式中,HBsAg、HBsAb、HBeAg、HBcAb阳性模式比例最高,为53.1% (51/96);HBsAg、HBsAb、HBeAb、HBcAb阳性模式所占比例为35.4% (34/96).HBeAg阳性组的HBV DNA阳性率为86%;HBeAg阴性组HBV DNA 阳性率为53.1%,两组差异显著(P<0.05).HBV DNA阳性样本中,HBeAg阳性组的平均病毒载量对数值高于HBeAg阴性组(5.08±1.12/ml vs 4.43±1.15/ml,P<0.05).在共存模式中,以C组(HBsAg≥2,HBsAb 1.0~1.9)HBV DNA阳性率最高,达61.5%(59/96),显著高于其他各组(P<0.05).结论 对于HBsAg与HBsAb共存模式的血清样品,可用荧光定量PCR技术检测HBV DNA来确定体内病毒是否处于复制状态和载量高低,以提高临床诊断的准确性和改进治疗方法.%HBV DNA copies for coexistent models of HBsAg and HbsAb were detected to discuss the application value for diagnosis and treatment of corresponding cases. Methods HBsAg,HBsAb,HBeAg, HBeAb and HBcAb were detected by ELISA and the statistical distribution of coexistent serum models of HBsAg and HBsAb were obtained. HBV DNA copies were determined for the coexistent serum samples by fluorescence quantitative PCR. Ninety -six cases of coexistent samples were divided into four groups ( A,B,C,D) according to the cut off values( CO) of HBsAg and HbsAb. Results Among the coexistent models of HBsAg and HBsAb, the positive model of HBsAg, HBsAb, HBeAg and HBcAb acounted for 53. 1% (51/96) , and the positive model of HBsAg, HBaAb, HBeAb and HBcAb acounted for 35.4% (34/96). Besides, the positive rate of HBV DNA was 86% in

  14. Riesgo de enfermedades infecciosas transmitidas por transfusión en el Valle del Cauca, Colombia.

    OpenAIRE

    Armando Cortés B.; Mauricio Beltrán; Beatriz Olaya; Marlene Hernández

    2009-01-01

    Se informa el potencial de riesgo para enfermedades infecciosas obtenidas a través de transfusión en el Valle del Cauca en 1997. Todas de las unidades de sangre colectadas en este departamento se tamizaron para los siguientes marcadores de infección: anticuerpos contra el virus de la inmunodeficiencia humana (anti-VIH-1,2), anticuerpos contra el virus de la hepatitis C (anti-VHC), antígeno de superficie de la hepatitis B (AgHBs) y anticuerpos contra Trypanosoma cruzi (anti-Tc), y parcialmente...

  15. [Comparison of the clinical performance of the ECLusys HBsAg II assay with the Lumipulse f and HISCL 2000-i HBsAg screening assays].

    Science.gov (United States)

    Sugiura, Aya; Iwahara, Kunihiro; Suga, Yasuyuki; Uchiyama, Sachinori; Maekawa, Masato

    2012-02-01

    We compared the ECLusys HBsAgII (ECL HBsAg) assay to the Lumipulse Forte (LPf HBsAg) and HISCL (HIS HBsAg) assays. Measurement of dilution panels for which the WHO HBsAg international reference panel was the parent specimen revealed that the ECL and HIS assays enabled detection to a theoretical level of 0.04 IU/mL, whereas the LPf assay enabled detection to a level of 0.08 IU/mL. In a specificity test using high RF positive specimens (n = 33), pregnancy specimens (n = 35), cytomegalovirus antibody positive specimens (n = 36), and high M protein positive specimens (n = 21) that were confirmed negative for HBsAg by the LPf assay, negative results were obtained for all specimens on the HIS assay, but the ECL assay yielded a positive result for one of the high RF positive specimens. This individual was suggested on further testing to be an HBV carrier who was strongly positive for HBc antibody. In HBsAg mutants detection test, the detection rate was 92.3% with the ECL assay and 69.2% with the HIS assay. In a correlation test using routinely collected clinical specimens (n = 155), including positive stock specimens, aside from the one case where the LPf assay gave a negative result but both the ECL and HIS assays gave positive results, all of the results were consistent for all specimens. The above results confirmed that the ECL assay is both highly sensitive and specific, and also enables a high rate of HBsAg mutant detection.

  16. Is Quantitative HBsAg Measurement a Reliable Substitute for HBV DNA Quantitation?

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Mahdavi

    2015-07-01

    Conclusion: There are many factors affecting the correlation between serum HBV DNA copy number and HBsAg level such as genotype of HBV virus, phase of infection, methods of measurement, HBeAg status, and drug and types of treatment procedures. Therefore, these factors should be considered in further studies dealing with the correlation between quantitative HBV DNA and HBsAg tests.

  17. Delayed clearance of serum HBsAg in compensated cirrhosis B

    DEFF Research Database (Denmark)

    Fattovich, G; Giustina, G; Sanchez-Tapias, J

    1998-01-01

    The aim of this study was to evaluate the incidence, prognostic factors and clinical significance of delayed clearance of serum HBsAg in compensated cirrhosis B.......The aim of this study was to evaluate the incidence, prognostic factors and clinical significance of delayed clearance of serum HBsAg in compensated cirrhosis B....

  18. Estudo da soroprevalência do AgHBs em gestantes da 15ª Regional de Saúde e da imunoprofilaxia para os recém-nascidos das gestantes AgHBs positivo = Study into the HBsAg seroprevalence in pregnant women from the 15th Health Regional and the immunoprophylaxia on the newborns of these HBsAg-positive women

    Directory of Open Access Journals (Sweden)

    Sonia Kaori Miyamoto

    2008-01-01

    Full Text Available Determinar a prevalência do AgHBs nas gestantes da 15ª Regional de Saúde (15ª R.S. atendidas no Laboratório de Ensino e Pesquisa em Análises Clínicas (Lepac, Universidade Estadual de Maringá, e verificar se foi solicitada a imunoprofilaxia para hepatite B aos recém-natos das gestantes AgHBs positivo, no período de janeiro de 1998 a dezembro de 2002. A pesquisa do AgHBs foi realizada pela técnica imunoenzimática IMxHBsAg e Axsym HBsAg (Laboratório Abbott. As solicitações de imunobiológicos especiais para a imunoprofilaxia da hepatite B ao CRIE foram obtidas na Seção de Epidemiologia da 15ª R.S. Foram analisadas 12.274 gestantes e a prevalência do AgHBs foide 1,0%. Dentre as 125 gestantes AgHBs positivo, foram solicitadas imunoprofilaxia para 32 (25,6% recém-nascidos. Ainda que a prevalência encontrada indique ser esta uma área de baixa endemicidade, os resultados reafirmam a importância da realização do diagnóstico da hepatite B no atendimento pré-natal, para adoção da imunoprofilaxia no recém-nascido.To determine HBsAg prevalence among pregnant women from the 15th Health Regional assisted in the Laboratório de Ensino e Pesquisa em Análises Clínicas (Lepac, Universidade Estadual de Maringá, and verify whether immunoprophylaxis with vaccine and immunoglobulin was requested for the newborns of HBsAg-positive women during the period from January 1998 to December 2002. The research about HBsAg was conducted using the immunoenzymatic IMxHBsAg (Abbot Lab and Axsym HBsAg (Abbot Lab techniques. The requests to the CRIE for specialimmunobiologic agents for Hepatitis B immunoprophylaxis were obtained in the Department of Epidemiology from the 15th Health Regional. The analysis includes 12,274 pregnant women, and the HBsAg prevalence was 1.0%. Among 125 HBsAg positive pregnant women, immunoprophylaxis was requested for 32 (25.6% newborns. Although the prevalence detected demonstrates this to be a low endemic area, the

  19. Kinetics of serum HBsAg in Chinese patients with chronic HBV infection with long-term adefovir dipivoxil treatment

    Institute of Scientific and Technical Information of China (English)

    Li Minran; Xi Hongli; Wang Qinhuan; Hou Fengqin; Huo Na; Zhang Xiaxia; Li Fang

    2014-01-01

    Background Knowledge on Hepatitis B surface antigen (HBsAg) kinetics in chronic hepatitis B (CHB) patients with longterm adefovir dipivoxil (ADV) treatment is limited.The aims of this study were to investigate HBsAg kinetics in patients with chronic hepatitis B virus (HBV) infection treated with long-term ADV and to evaluate different characteristics between patients with and without HBsAg loss.Methods We retrospectively evaluated HBsAg kinetics in 24 Chinese patients with chronic HBV infection who achieved continuous virologic suppression during ADV therapy.HBV genotype was determined at baseline.Liver biochemistry,hepatitis B e antigen status,serum HBV DNA,and HBsAg levels were measured at baseline,6 months,and once every year thereafter.Results Of these 24 patients,3,1,and 20 patients were followed up for 3,5,and 6 years,respectively.Baseline serum HBsAg level had a moderate correlation with baseline HBV DNA level (r=0.52,P=0.01).The median rate of HBsAg reduction during the therapy period was 0.08 Ig IU·ml-1·y-1.Baseline serum HBsAg level was significantly higher than other time points (P ranges from 0.046 to 0.002).The HBsAg reduction rate during the first year was similar to that in other years (P>0.05).The HBsAg reduction rate during the first year in patients with eventual HBsAg loss was significantly faster than that in patients without HBsAg loss (P=0.005).Conclusions Serum HBsAg levels in Chinese CHB patients receiving long-term ADV demonstrated a gradual reduction.Patients with eventual HBsAg loss had a significantly faster HBsAg reduction rate during the first year than those without HBsAg loss.

  20. Access免疫分析仪检测HBsAg的评价

    Institute of Scientific and Technical Information of China (English)

    何思春; 王利君

    2006-01-01

    目的评价Access免疫分析仪对血清HBsAg的测定.方法利用Access免疫分析仪及配套试剂检测血清HBsAg.结果 Access免疫分析仪测定HBsAg的灵敏度为0.1ng/ml,HBsAg在0.3~100ng/ml范围内线性良好;批内CV 3.97%~10.77%,批间CV 4.34%~14.48%;溶血显著影响HBsAg测定结果.抗-HBs、HBeAg、抗-HBe与HBsAg无交叉反应.结论 Access免疫分析仪测定HBsAg快速、灵敏、重复性好、线性范围宽、特异性强,可用于乙型肝炎病毒感染的确认.

  1. Construction and immunological evaluation of truncated hepatitis B core particles carrying HBsAg amino acids 119-152 in the major immunodominant region (MIR).

    Science.gov (United States)

    Su, Qiudong; Yi, Yao; Guo, Minzhuo; Qiu, Feng; Jia, Zhiyuan; Lu, Xuexin; Meng, Qingling; Bi, Shengli

    2013-09-13

    Hepatitis B capsid protein expressed in Escherichia coli can reassemble into icosahedral particles, which could strongly enhance the immunogenicity of foreign epitopes, especially those inserted into its major immunodominant region. Herein, we inserted the entire 'α' antigenic determinant amino acids (aa) 119-152 of HBsAg into the truncated HBc (aa 1-144), between Asp(78) and Pro(79). Prokaryotic expression showed that the mosaic HBc was mainly in the form of inclusion bodies. After denaturation with urea, it was dialyzed progressively for protein renaturation. We observed that before and after renaturation, mosaic HBc was antigenic as determined by HBsAg ELISA and a lot of viruslike particles were observed after renaturation. Thus, we further purified the mosaic viruslike particles by (NH4)2SO4 precipitation, DEAE chromatography, and Sepharose 4FF chromatography. Negative staining electron microscopy demonstrated the morphology of the viruslike particles. Immunization of Balb/c mice with mosaic particles induced the production of anti-HBs antibody and Th1 cell immune response supported by ELISPOT and CD4/CD8 proportions assay. In conclusion, we constructed mosaic hepatitis core particles displaying the entire 'α' antigenic determinant on the surface and laid a foundation for researching therapeutic hepatits B vaccines.

  2. Clinical Significance of Establishing Relationship between the HBsAg Test and HBV DNA%乙肝五项测定结果与HBV DNA的关系及其临床意义

    Institute of Scientific and Technical Information of China (English)

    李昕; 张荣波

    2011-01-01

    Objective To explore the internal relationship and the clinical significance of HBVM( HBsAg, Anti-HBs,HBeAg,Anti-HBe, Anti-HBc )and HBV DNA( Hepatitis B Virus DNA ). Methods HBVM and HBV-DNA of 300 HBV patients were examined by enzyme linked immunosorbent assay( ELISA )and quantitative fluorescence PCR technique respectively. Results Of all the clinical results of 300 HBV patients,110 patients showed HBsAg( + ),HBeAg( + ),HBcAb( + )( HBV DNA positive rate 99. 1% ),85 patients showing HBsAg( + ), HBeAb( + ),HBcAb( + )( HBV DNA positive rate 69.4% ), 39 patients showing HBsAg( + ),HBcAb( + )( HBV DNA positive rate 53.8% ),53 patients showing HBeAb( + ),HBcAb( + )( HBV DNA positive rate 35.8% ). HBV DNA positive rate of HBsAg( + ),HBeAg( + ),HBcAb( + ) and HBsAg( + ), HBeAb( + ), HBcAb( + ) were different significantly( x2 = 35. 406, P < 0.05 ). Conclusion The results of HBsAg and HBV-DNA are closely related with different clinical meanings,which makes it necessary to combine the two results for a correct diagnosis and treatment.%目的探讨乙型肝炎(乙肝)五项结果和HBV DNA检出情况间的关系和临床意义.方法运用聚合酶链反应法检测300例乙肝血清的HBV DNA,并用酶联免疫吸附实验法进行乙肝五项的测定,对结果进行比较分析.结果 300例乙肝门诊的患者中乙肝五项的结果为110例HBsAg(+)、HBeAg(+)、HBcAb(+)(大三阳),其HBV DNA的阳性率为99.1%,85例HBsAg(+)、HBeAb(+)、HBcAb(+)(小三阳),其HBV DNA的阳性率为69.4%,39例HBsAg(+)、HBcAb(+),其HBV DNA的阳性率为53.8%,53例HBeAb(+)、HBcAb(+),其HBV DNA的阳性率为35.8%.大三阳和小三阳的HBV DNA阳性率比较差异有统计学意义(χ2=35.406,P<0.05).结论乙肝五项的结果与HBV DNA的结果有着密切的联系,并且都具备各自的临床意义,因此两者必须结合才能正确地对乙肝患者的病情作出正确分析和判断.

  3. Hepatitis B virus and hepatitis D virus in blood donors from Argentina: circulation of HBsAg and reverse transcriptase mutants.

    Science.gov (United States)

    Delfino, Cecilia María; Gentile, Emiliano Alberto; Castillo, Amalia Inés; Cuestas, María Luján; Pataccini, Gabriela; Cánepa, Camila; Malan, Richard; Blejer, Jorgelina; Berini, Carolina; Eirin, María Emilia; Pedrozo, Williams; Oubiña, José Raúl; Biglione, Mirna Marcela; Mathet, Verónica Lidia

    2014-05-01

    In Argentina, current procedures to ensure the safety of the blood supply for transfusion include the serologic detection of specific blood-borne infections. The aim of this study was to evaluate the prevalence and the genetic diversity of hepatitis B virus (HBV) and hepatitis D virus (HDV) in blood donor populations from two distantly located Argentine regions. Data from 56,983 blood donations from the Favaloro Foundation, in the city of Buenos Aires (Central Region), and the Central Blood Bank of Misiones Province (Northeast Region) were analyzed. Samples that were reactive for HBsAg were analyzed for HBV-DNA characterization and HDV serological and molecular analysis. The HBV prevalence was 0.12 % for HBsAg and 1.68 % for anti-HBc antibodies in Buenos Aires, and 0.73 % and 8.55 %, respectively, in Misiones. Seventy-seven HBsAg-reactive samples were analyzed by polymerase chain reaction for HBV-DNA. Subgenotypes A2, B2, C2, F1b and F4 (Buenos Aires) and F1b and D3 (Misiones) were detected. Several mutations within the major hydrophilic region of HBsAg, the reverse transcriptase, the basal core promoter, and the precore/core were detected. HDV genotype 1 was identified in Buenos Aires. This study confirms the circulation of several HBV subgenotypes, as well as known and newly identified variants, and the presence of HDV1 in this population. A thorough investigation has to be carried out to evaluate the clinical importance of some of the documented mutations as well as those detected in the HDV1 case.

  4. Seroprevalence of HDV among non-hospitalized HBs Ag positive patients from KPK-region of Pakistan

    Institute of Scientific and Technical Information of China (English)

    Ismail Jalil; Muhammad Arshad; Zara Rafaque; Fazle Raziq; Robina Wazir; Sajid Malik; Javid Iqbal Dasti

    2016-01-01

    Objective: To study the seroprevalence of hepatitis B virus(HBV) and hepatitis delta virus(HDV) infections in patients visiting outpatient department of a major tertiary care hospital in Khyber Pakhtunkhwa region of Pakistan.Methods: Blood samples were collected from non-hospitalized patients. Serological analysis was done by ELISA and viral DNA was amplified by PCR. The amplified DNA was analyzed by agarose gel electrophoresis.Results: Altogether, 946 blood samples were screened, overall percentage of HBs Agpositive patients remained 22.41%(prevalence: 224.10/1 000; CI: 0.197 5 ± 0.250 7) with the highest incidence rates among relatively younger age groups(20–29 years). The prevalence of HBV–HDV co-infection was found to be 46.75/1 000; CI: 0.031 8 ± 0.061 7.In HBs Ag-positive patients, anti-HBc-total was detected in 86.79% while 25.00% were positive for anti-HBc-immunoglobulin M. Similarly, among these patients, HBV DNA was detected in 64.13% and 10.85% were co-infected with HDV. Different symptoms were associated with the prevailing infection, including malaise(62%), anorexia(66%) and fatigue(73%). The most commonly associated symptom was abdominal discomfort. Among these patients, certain risk factors, including surgery, visit to dentist and intravenus infusions were frequently associated with the infection(x~2= 95.23; df = 11; P < 0.000 1).Conclusions: Overall, this study confirmed higher prevalence of active HBV/HDV infection, among young patients from Khyber Pakhtunkhwa region having no prior history of viral hepatitis.

  5. Long-term effects of maternal positive-HBsAb on children with hepatitis B vaccine%孕妇乙肝表面抗体对儿童乙肝疫苗接种效果的长期影响

    Institute of Scientific and Technical Information of China (English)

    吴限; 高夕雷; 吴凤会; 钱雷

    2013-01-01

    OBJECTIVE To investigate long-term effects of maternal positive-HBsAb on children with hepatitis B vaccine.METHODS 215 pairs of mothers and children were enrolled including 106 anti-HBs positive mothers and 109 anti-HBs negative mothers.All infants were routinely vaccinated against hepatitis B based on 0-,1-and 6-month schedule.At the age of one and half years,the concentration of serum anti-HBs of childhood was observed.RESULTS All 106 anti-HBs positive mothers transferred anti-HBs to their infants and 84.9% of the infants had higher anti-HBs concentrations than their mothers.One and half years after vaccination with three doses of hepatitis B vaccine,the positive rate and geometric mean concentration (GMC) of anti-HBs in infants with maternal anti-HBs compared with those in infants without maternal antibody were 89.6% vs 87.1% (P> 0.05) and 79.5 IU/L vs 78.7 IU/L (P> 0.05) respectively.CONCLUSION Maternal anti-HBs in infants do not inhibit the long-term immunogenicity of hepatitis B vaccine.%目的 了解孕妇乙肝表面抗体(抗HBs)对儿童乙肝疫苗接种效果的长期影响.方法 研究对象为106例抗HBs阳性和109例抗HBs阴性孕妇及其婴儿,检测孕妇产时外周血和新生儿脐带血抗HBs浓度,婴儿出生后接种5ug乙肝疫苗(0、1、6月),在儿童1岁半时检测血液抗HBs浓度.结果 抗HBs阳性孕妇的新生儿脐带血中均含有抗HBs,84.9%的新生儿脐带血抗HBs浓度高于母亲外周血,抗HBs阳性孕妇组和抗HBs阴性孕妇组的儿童在1岁半时外周血抗HBs阳性率分别为89.6%和87.1% (P> 0.05),抗HBs浓度几何均数分别为79.5 IU/L和78.7 IU/L (P>0.05),差异均无显著性.结论 母亲乙肝表面抗体不影响儿童乙肝疫苗接种的长期效果.

  6. [A simple ELISA method for the detection of HBsAg: Organon Teknika HBsAg Uniform II screening and confirmation test. Comparative study using the HBsAg Hapanostika method. A multicenter study].

    Science.gov (United States)

    Pár, A; Mihály, I; Kömives, K

    1994-09-25

    An one-step enzyme-linked immunoabsorbent method, named as HBsAg Uniform II has been described for the detection of serum HBsAg, and a comparison was made with a widely used ELISA technique HBsAg Hepanostika test, to evaluate sensitivity, specificity and reproducibility of the method. A total of 531 serum samples from patients with liver disease and with renal failure, as well as 1065 samples from blood donors have been investigated. While the sensitivity of Uniform II vs. Hepanostika was 99.5% vs. 72.7%, the specificity was 99.2% of both methods. The positive predictive values did not differ (99.5% vs. 99.2%), however, the negative predictive values were 99.2% vs. 71.7%, respectively, in favour of Uniform II test. The Uniform II confirmatory test confirmed the positive HBsAg results in 94%, this rate was 74% using Hepanostika system. The new method proved to be a simple, quick, reliable test, which can be useful as a valuable tool in both the clinical diagnosis and blood donor screening.

  7. Open multicenter trial with Madopar HBS in parkinsonian patients. Preliminary assessment after short-term treatment.

    Science.gov (United States)

    Jansen, E N; Meerwaldt, J D; Heersema, T; van Manen, J; Speelman, J D

    1987-01-01

    In 23 patients with idiopathic Parkinson's disease presenting with severe fluctuations in motor performance and 'on-off' phenomena after long-term treatment with levodopa, the standard form of Madopar was replaced by the controlled-release form Madopar HBS. The Meerwaldt's patient card has been used to evaluate the frequency and intensity of response swings. Only 2 patients, who suffered from clear-cut 'end-of-dose' deterioration, significantly benefited by this switch from standard Madopar to Madopar HBS. Eight patients had a minimal or not essential improvement of the parkinsonian symptomatology and/or of the response fluctuations. Thirteen patients returned to their previous standard Madopar treatment after deterioration of parkinsonian symptoms or increase of dyskinesia under the HBS treatment. The overall increase in dosage of levodopa with Madopar HBS was 54% in comparison with the initial standard Madopar dosage.

  8. Comparative study of methods of detection of hepatitis ′B′ surface antigen (HBsAg.

    Directory of Open Access Journals (Sweden)

    Parab V

    1989-04-01

    Full Text Available The serum samples were collected from 52 patients of acute viral hepatitis and 235 hospital staff from Kasturba Hospital for Infectious Diseases. HBsAg was detected in their sera by counter-immuno-electrophoresis (CIEP, reverse passive hemogglutination (RPHA and by micro-enzyme-linked-immunosorbent assay (ELISA technique. Among the patients, HBsAg was detected in 12 cases (23% by CIEP, in 18 cases (34% by RPHA and in 23 patients (45% by ELISA. In the hospital staff, HBsAg was detected in 4 samples (1.7% by CIEP, in 8 samples (3.5% by RPHA and in 32 samples (13.5% by ELISA. Thus ELISA was found to be the most sensitive technique in detecting HBsAg.

  9. Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales, Phaeophyta)

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A transformation model for Laminaria japonica was established from 1993 to 1998, on the basis of which the transgenic kelp with heterologous gene encoding hepatitis B surface antigen (HBsAg) was obtained by using the micro- particle bombardment transformation method. Results of quantitative ELISA showed that HBsAg in transgenic kelp was 0.529 μg/mg soluble proteins on average and the highest value was 2.497 μg/mg, implying that recombinant HBsAg had natural epitope. Further support for the integration of HBsAg gene into kelp genome was obtained by PCR- Southern and total DNA hybridization. Prospect of kelp bioreactor producing high value materials such as edible HBV vaccine was discussed as well.

  10. "Anti-HBc alone" in human immunodeficiency virus-positive and immuno-suppressed lymphoma patients

    Institute of Scientific and Technical Information of China (English)

    Yu Xuan Koo; Daniel SW Tan; Iain BH Tan; Richard Quek; Miriam Tao; Soon Thye Lim

    2009-01-01

    Hepatitis B virus (HBV) infection is endemic in various parts of the world. A proportion of patients have resolved prior exposure to HBV, as evidenced by the clearance of circulating hepatitis B surface antigen and the appearance of antibody to hepatitis B core antigen (anti-HBc), which could produce protective antibody to hepatitis B surface antigen (anti-HBs). With time, anti-HBs in some patients may become negative. Such patients are described as having occult HBV infection or "anti-HBc alone". In the context of immunodeficient patients, such as HIV patients or lymphoma patients undergoing immunosuppressive immunotherapy, the lack of protective anti-HBs may increase the risk of hepatitis B reactivation. Serum HBV DNA testing may be necessary in "anti-HBc alone" patients, to detect patients at a high risk of developing HBV infection allowing appropriate prophylactic management.

  11. HBsAg, HBcAg, and combined HBsAg/HBcAg-based therapeutic vaccines in treating chronic hepatitis B virus infection

    Institute of Scientific and Technical Information of China (English)

    Sheikh Mohammad Fazle Akbar; Mamun Al-Mahtab

    2013-01-01

    BACKGROUND: As  the  host  immunity  is  diminished  in patients with chronic hepatitis B (CHB), different approaches have  been  used  to  up-regulate  their  immune  responses  to produce therapeutic effects. But, cytokines, growth factors and polyclonal  immune  modulators  could  not  exhibit  sufficient therapeutic  effects  in  these  patients.  Immune  therapy  with HBV-related antigens (vaccine therapy) has been used in CHB patients. But there is a paucity of information about the design of HBV antigen-based immune therapy in these patients. DATA  SOURCE: Preclinical  and  clinical  studies  on  immune therapy with HBsAg-based vaccine, HBcAg and combination of HBsAg/HBcAg-based vaccines have been discussed. RESULTS: HBsAg-based  prophylactic  vaccine  was  used  as an  immune  therapeutic  agent  in  CHB  patients;  however, monotherapy  with  HBsAg-based  immune  therapy  could not  lead  to  sustained  control  of  HBV  replication  and/or  liver damages.  HBsAg-based  vaccine  was  used  as  a  combination therapy  with  cytokines,  growth  factors,  and  antiviral  drugs. HBsAg-based  vaccine  was  also  used  for  cell-based  therapy. However,  satisfactory  therapeutic  effects  of  HBsAg-based vaccine could not be documented in CHB patients. In the mean time, evidences have supported that HBcAg-specific immunity is  endowed  with  antiviral  and  liver  protecting  capacities  in CHB  patients.  Recent  data  concentrate  on  the  clinical  use  of combined HBsAg- and HBcAg-based vaccines in CHB patients. CONCLUSION: Antigen-based  immune  therapy

  12. SISTEMA INMUNE Y GENÉTICA: UN ABORDAJE DIFERENTE A LA DIVERSIDAD DE ANTICUERPOS.

    Directory of Open Access Journals (Sweden)

    Matta Camacho Nubia Estela

    2011-12-01

    Full Text Available RESUMEN Es común encontrar en los libros de inmunología o de genética un capítulo con el título de “sistema inmune y genética”, sin embargo su asociación se centra en cómo la generación de anticuerpos rompió el paradigma “un gen, una proteína”, pues en el caso de la producción de anticuerpos, un gen produce millones de proteínas. El sistema inmune tiene muchos vínculos con la genética y la herencia; esta asociación se da porque cualquier sustancia o compuesto que produzca un organismo, es un antígeno potencial cuando es reconocido como extraño por el sistema inmune de otro organismo, sea este de la misma o de diferente especie. La producción de proteínas que potencialmente pueden ser antigénicas están ligadas al genotipo del individuo. La capacidad de responder y el tipo e intensidad de respuesta a los antígenos también ha sido demostrado que está correlacionado con el genotipo del individuo en cuestión, así como deficiencias en las respuestas inmunes pueden estar asociadas con mutaciones o polimorfismos genéticos que resultan en la susceptibilidad a enfermedades infecciosas. Este artículo se busca presentar ejemplos de la relación del sistema inmune y la genética, en la misma dirección de la conferencia ofrecida para la cátedra de Sede “Todo lo que usted quiere saber de genética y nunca se atrevió a preguntar”. Palabras claves: HLA, respuesta inmune, grupos sanguíneos, antígenos. ABSTRACT It is common to find in immunology or genetic books a chapter entitled “immune system and genetics”; this association focuses on how the generation of antibodies broke the paradigm “one gene, one protein”, since in this case one gene generates millions of proteins. However, the immune system has many more links to genetics and heredity. For example, any substance or compound that an organism produces is a potential antigen, when it is recognized as foreign by the immune system of another

  13. Determination of HBsAg subtypes in Western Siberian part of Russia.

    Science.gov (United States)

    Netesova, I G; Swenson, P D; Osipova, L P; Gubina, M A; Posukh, O L; Netesov, S V

    2003-10-01

    A set of monoclonal antibodies with specificity for hepatitis B surface antigen (HBsAg) was used for subtyping this antigen in sera from indigenous natives, blood donors, and drug users in Western Siberia with a modified commercial enzyme immunoassay kit for HBsAg detection. Three subtypes of HBsAg in a ratio of 36 (78%) ayw2:8 ayw3varB (18%):2 (4%) adw2 were found in 46 (100%) HBsAg-positive sera of different aboriginal populations of Western Siberia: the Tundra Nenets, Northern Khanty, Southern Altaians, and Kazakhs. Four subtypes of HBsAg in a ratio of 81 (57%) ayw2:58 (15 ayw3varA and 43 ayw3varB; 44%):2 (1%) adw2 were detected in 141 (100%) samples of blood donors from ten cities of Western Siberia. Three subtypes of HBsAg in a ratio of 34 ayw3:(both variants, 33 ayw3varA and 1 ayw3varB; 97.1%):1 (2.9%) ayw2 were found in blood of 35 injection drug users in Novosibirsk.

  14. Evaluación de la respuesta de anticuerpos hacia antígenos de Pseudomonas aeruginosa.

    Directory of Open Access Journals (Sweden)

    Aniel Moya

    2007-04-01

    Full Text Available Pseudomonas aeruginosa es un patógeno extracelular que genera una respuesta de anticuerpos específicos con utilidad para el diagnóstico y vacunas. En el presente estudio nos propusimos evaluar en suero humano los niveles de anticuerpos contra antígenos relevantes de P. aeruginosa. Realizamos la determinación de anticuerpos IgG contra tres exoenzimas, consideradas como factores de virulencia de mayor importancia en infecciones. Este resultado dio paso a la evaluación del reconocimiento de IgG e IgA hacia antígenos de la envoltura celular bacteriana por ELISA de células enteras. Todos los sueros evaluados mostraron títulos de IgG e IgA superiores a los individuos sanos, con excepción de dos muestras de pacientes que no mostraron alto título. Este ensayo permitió analizar el nivel de reconocimiento hacia los antígenos más expuestos de la bacteria que incluyen principalmente LPS y proteínas de membrana externa. Se encontró diferencias entre los valores de densidad óptica a 450 nm de individuos sanos y enfermos. El método usado permitió seleccionar dos sueros de pacientes de diferentes tipos de infecciones que fueron comparados por Western blot. Se observó que aunque los sueros tenían reacción hacia distintos serotipos de P. aeruginosa, la intensidad del reconocimiento variaba según el tipo de infección.

  15. Encefalitis por anticuerpos contra el receptor de NMDA: experiencia con seis pacientes pediátricos. Potencial eficacia del metotrexato

    Science.gov (United States)

    Bravo-Oro, Antonio; Abud-Mendoza, Carlos; Quezada-Corona, Arturo; Dalmau, Josep; Campos-Guevara, Verónica

    2016-01-01

    Introducción La encefalitis por anticuerpos contra el receptor de N-metil-D-aspartato (NMDA) es una entidad cada vez más diagnosticada en edad pediátrica. A diferencia de los adultos, en muchos casos no se asocia a tumores y las manifestaciones iniciales en niños más frecuentes son crisis convulsivas y trastornos del movimiento, mientras que en los adultos predominan las alteraciones psiquiátricas. Casos clínicos Presentamos seis casos pediátricos confirmados con anticuerpos contra la subunidad NR1 del receptor de NMDA en suero y líquido cefalorraquídeo. Cinco de los casos comenzaron con crisis convulsivas como manifestación clínica inicial antes de desarrollar el cuadro clásico de esta entidad. En todos los casos se utilizaron esteroides como primera línea de tratamiento, con los que sólo se observó control de las manifestaciones en uno, por lo que el resto de los pacientes requirió inmunomoduladores de segunda línea. Todos los pacientes recibieron metotrexato como tratamiento inmunomodulador para evitar recaídas y la evolución fue a la mejoría en todos ellos. Conclusiones En nuestra serie de pacientes con encefalitis por anticuerpos contra el receptor de NMDA, ninguno se asoció a tumores. Todos los casos recibieron metotrexato por lo menos durante un año, no observamos eventos adversos clínicos ni por laboratorio, ni hubo secuelas neurológicas ni recaídas durante el tratamiento. Aunque es una serie pequeña y es deseable incrementar el número y tiempo de evolución, consideramos el metotrexato una excelente alternativa como tratamiento inmunomodulador para esta patología. PMID:24150952

  16. Anticuerpos séricos contra la enfermedad de Newcastle e Influenza Aviar en aves rapaces de Chile

    OpenAIRE

    Daniel González-Acuña; Álvaro Gaete; Lucila Moreno; Karen Ardiles; Fabiola Cerda-Leal; Christian Mathieu; René Ortega

    2012-01-01

    RESUMENObjetivo. Detectar la presencia de anticuerpos séricos sanguíneos contra los virus de la Enfermedad de Newcastle (ENC) e Influenza aviar (IA), para comprender la contribución de las aves silvestres en la transmisión de estos virus en Chile. Materiales y métodos. Se analizaron 63 aves pertenecientes a los órdenes Falconiformes y Strigiformes desde centros de rehabilitación de aves de las zonas central y sur de Chile. Se realizaron las pruebas de inhibición de la hemoaglutinación (IHA) p...

  17. Descripción de los anticuerpos, y manifestaciones clínicas de pacientes con polimiositis y dermatomiositis

    OpenAIRE

    Vega Castro, Kelly Patricia

    2014-01-01

    Introducción: La polimiositis y dermatomiositis, son enfermedades del grupo de miopatías inflamatorias, su curso es crónico, etiología autoinmune, tienen debilidad muscular simétrica de inicio proximal y la dermatomiositis se asocia a manifestaciones cutáneas. Genera importante situación de discapacidad física y emocional, costo social y económico elevado. La asociación de los anticuerpos circulantes con las manifestaciones clínicas, posibilidad de complicaciones y pronóstico ha tenido en los...

  18. Procedimiento para la obtención de anticuerpos monoclonales a partir de muestras complejas de antígenos

    OpenAIRE

    Fraile de Paz, Sofía; Zafra-Amorós, Olga; Jiménez, José Ignacio; Lorenzo Prieto, Víctor de

    2011-01-01

    La presente invención se refiere a un proceso de selección, en un solo paso, de dominios variables de anticuerpos, preferiblemente de camello, unidos a fagos, de gran afinidad y especificidad para un antígeno concreto o diversas proteínas que comparten epítopos comunes. Además el procedimiento incluye de forma preferente una amplificación de los fagos que comprenden los dominios variables de interés, mediante la infección de bacterias por dichos fagos, lo que permite la obtención de un elevad...

  19. Producción de un anticuerpo IgY específico contra el antígeno CD41 humano

    Directory of Open Access Journals (Sweden)

    Esteban J. Gutiérrez Calzado

    2009-01-01

    Full Text Available Desde hace dos décadas, la comunidad científica muestra un gran interés hacia la generación de anticuerpos en gallinas ponedoras por las ventajas que estas aportan al compararse con otros métodos tradicionales para este fin, sobre todo, en el hecho de lograrse en ellas, mejores respuestas de anticuerpos hacia antígenos de mamíferos muy conservados en la filogenia entre los que se encuentran glicoproteínas de membrana celular. El objetivo de este trabajo fue la generación de anticuerpos específicos contra la glicoproteína de membrana plaquetaria humana gpIIb/IIIa (antígeno CD41 en gallinas ponedoras. Para este propósito, se utilizaron gallinas de 22 semanas de edad, a las que se les aplicaron dosis de 20 ¿g de una preparación inmunopurificada de este antígeno plaquetario, emulsionada con adyuvante completo e incompleto de Freund para una primera y segunda inmunización respectivamente, a intervalos de un mes. Se cosecharon los huevos diariamente de los animales inmunizados a partir de un día antes de esta aplicación hasta 30 d posteriores a la segunda inyección, se procesaron estos para la extracción de los anticuerpos específicos presentes en ellos y se obtuvo la dinámica de producción de anticuerpos por el método clásico de Ouchterlony que se enfrentó a una preparación pura del antígeno. Las respuestas de anticuerpos obtenidas resultaron en títulos adecuados susceptibles de investigar en usos posteriores en la Medicina Transfusional específicamente para el análisis de la calidad de plasmas.

  20. POLYCLONAL ANTIBODIES TO DETECT THE CP-RT PROTEIN OF Potato Mop-Top VIRUS ANTICUERPOS POLICLONALES PARA LA DETECCIÓN DE LA PROTEÍNA CP-RT DEL Potato Mop-Top VIRUS

    Directory of Open Access Journals (Sweden)

    Derly Andrade Molina

    2012-06-01

    Full Text Available Abstract. Potato mop-top virus (PMTV causes an important re-emerging disease in potato crops in Colombia due to the increased incidence of its protozoan vector Spongospora subterranea f.sp. subterranea (Sss, the causing agent of Powdery scab disease. For an accurate detection of PMTV it is recommended to combine different diagnostic tests and evaluate multiple samples per plant or tissue. In order to increase the number of available tools for detection of PMTV, antibodies targeting the RT domain of the CP-RT protein were developed in this work. Sequencing of the RT domain from the colombian strain R25 was achieved and using bioinformatic analysis, a potential antigenic region was identified. A peptide mimicking the antigenic region was inoculated in rabbits for the production of polyclonal antibodies. The antibodies were tested by ELISA using Nicotiana benthamiana bait-plants infected with Sss cystosori and potato plants collected in La Unión (Antioquia. The validity of serological tests was confirmed by RT-PCR. A complete sequence of the RT domain and 441 nt of the CP gene were obtained. Phylogenetic analysis identified strain R25 as closely associated to the PMTV lineage distributed worldwide. A total of 19.26 mg of anti-CP-RT polyclonal antibodies useful in detecting PMTV in infected plants were obtained. As CP-RT is involved in transmission of PMTV by Sss, these antibodies will be useful for supporting not only diagnostic programs but also basic and epidemiologic studies aimed at understanding interactions between PMTV and Sss.Resumen. El Potato mop-top virus (PMTV es uno de los virus re-emergentes en los cultivos de papa de Colombia, como resultado del aumento de la incidencia de su vector natural, el protozoo Spongospora subterranea f.sp. mamarinm@unal.edu.co (Sss, agente causal de la Sarna polvosa de la papa. Para la detección del virus se recomienda la realización simultánea de diferentes pruebas, así como la evaluación de m

  1. 聚集性HBV感染家系中父亲HBsAg阳性家庭传播特点分析%Characteristics of HBV transmission in families with HBsAg-positive fathers and familial clustering of HBV infection

    Institute of Scientific and Technical Information of China (English)

    杨瑗; 金李; 何英利; 刘锦锋; 王静; 王科; 马晓华; 李倩; 冯玉岭

    2016-01-01

    Objective To investigate the characteristics of hepatitis B virus (HBV) transmission among family members in families with familial clustering of HBV infection and poor outcomes,as well as the prevalence and distribution characteristics of HBsAg in offspring with different parental HBsAg status.Methods The general information of each member in families with poor outcomes were collected from 2007 to 2010,and serological test was performed to analyze the prevalence and distribution of HBsAg in family members.The chi-square test or Fisher's exact test was used to analyze and compare the sex of offspring and the prevalence of HBsAg in them in 266 nuclear families with different paternal and maternal HBsAg status.Results The positive rates of HBsAg in parents,siblings,children,and spouses of the probands were 20%,88.2%,76.8%,and 9.5%,respectively.The nuclear families with HBsAg-positive fathers and HBsAg-negative mothers had a significantly increased proportion of male offspring (male/female ratio =2.02) compared with those with HBsAg-positive mothers and HBsAg-negative fathers (1.22) or those with HBsAg-negative fathers and mothers (0.96).In addition,in the nuclear families with HBsAg-positive fathers and HBsAg-negative mothers,the male offspring had a significantly higher HBsAg positive rate than female offspring (37.4% vs 13.8%),while in those with HBsAg-positive mothers and HBsAg-negative fathers or those with HBsAg-negative fathers and mothers,HBsAg positive rate showed no significant difference between male and female offspring.Conclusion In families with familial clustering of HBV infection and poor outcomes,mother-to-child transmission is still the major route of HBV transmission,but father-to-child transmission also plays a role in HBV transmission in this special population.Positive HBsAg in fathers is associated with the increased proportion of male offspring,and father-to-son transmission of HBV is higher than father-to-daughter transmission

  2. EVIDENCIA DE LA PRESENCIA DE ANTICUERPOS DE Mycoplasma hyopneumoniae EN CERDOS PROVENIENTES DE GRANJAS DE CRIANZA ARTESANAL DEL SUR DE LIMA.

    OpenAIRE

    Ibarra C., Marco; Practica privada.; Noé M., Norma; Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Alvarado S., Arnaldo; Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima. Apdo. 41-0068; Perales C., Rosa; Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima. Apdo. 41-0068

    2014-01-01

    Se realizó un estudio para determinar la presencia de anticuerpos de Mycoplasma hyopneumoniae en cerdos provenientes de granjas de crianza artesanal, mediante la detección de anticuerpos, utilizando el ensayo inmuno absorbente ligado a enzimas(ELISA). Ciento sesenta animales de 8 a 9 meses de edad procedentes de granjas de 4 distritos ubicados al Sur de Lima, fueron muestreados al momento de ser beneficiados en 2 carmales frigoríficos del valle de Lima. Se obtuvo una frecuencia de animales po...

  3. Utilidad de las muestras de sangre total desecada en papel de filtro en la detección de anticuerpos antitoxoplasma

    Directory of Open Access Journals (Sweden)

    Joaquim Gascon

    1989-04-01

    Full Text Available Se compara la técnica de Aglutinación Directa (AD utilizando muestras de sangre total desecada en papel de filtro, con la técnica de ELISA y la misma AD utilizando muestras de suero de los mismos pacientes, para la detección de anticuerpos antitoxoplasma. Los resultados muestran la validez del método de la sangre desecada en papel de filtro para la detección de anticuerpos antitoxoplasma con la técnica de AD, y se considera su utilidad en los estudios epidemiológicos de campo.

  4. 污染HBsAg的口腔器械消毒方法的实验研究%Experimental study on sterilizing methods of the dental instruments contaminated with HBsAg

    Institute of Scientific and Technical Information of China (English)

    杨聚才; 安银东; 黄萍; 王军玲

    1999-01-01

    目的:探讨一种能在较短时间内灭活HBV的消毒方法.方法:采用不同浓度的戊二醛擦拭消毒已污染HBsAg阳性血清的口腔专科常用诊疗器械,用ELISA法检测HBsAg.结果:用20~30ml/L戊二醛消毒剂擦拭消毒1遍已污染HBsAg的口腔器械,HBsAg的灭活率为8%,而采用擦拭消毒3遍的方法,HBsAg的灭活率为92%~100%.结论:选用较高浓度的戊二醛消毒剂擦拭消毒3遍,可以达到灭活HBsAg的目的.

  5. 溶血、脂血、黄疸对HBsAg胶体金法检测的影响%Influences of hemolysis,blood lipid, jaundice on HBsAg detection

    Institute of Scientific and Technical Information of China (English)

    张孔志; 顾猛; 吴海燕; 王拥军

    2009-01-01

    目的 测试溶血、脂血、黄疸对HBsAg胶体金法检测的影响.方法 将不同程度溶血、脂血、黄疸HBsAg含量为5ng/ml的标本用HBsAg胶体金法进行检测,同时将HBsAg含量为5ng/ml、2.5ng/ml溶血、脂血、黄疸三项指标正常的标本作为对照,通过仪器检测OD值来比较三者对检测结果的影响.结果 TBiL为80mmol/L、TBiL为160mmol/L和TG为2.56mmol/L,HBsAg含量为5ng/ml的标本对HBsAg胶体金法检测影响不明显(P>0.05);TG为3.68mmol/L,HBsAg含量为5ng/ml的标本对HBsAg胶体金法检测影响较明显(P<0.05);Hb为5g/L、10g/L和20g/L HBsAg含量为5ng/ml的标本对HBsAg胶体金法检测影响显著(P<0.01).结论 溶血和严重脂血对HBsAg胶体金法检测的影响较大.黄疸对HBsAg胶体金法检测的影响不明显.

  6. MEIA检测低水平血清HBsAg及结果分析%Determination of Low Level HBsAg in Serum by Microparticle Enzyme Immunoassay and Analysis of Results

    Institute of Scientific and Technical Information of China (English)

    谭德安; 龙智钢; 罗胜权; 马双双

    2006-01-01

    目的 了解低水平血清乙肝病毒表面抗原(HBsAg)的人群分布及其相关乙肝病毒血清标志物模式特征.方法 微粒子酶免疫分析技术(microparticle enzyme immunoassay,MEIA)测定8 089例非肝炎病区住院患者血清HBsAg及其表面抗体(抗-HBs)、乙肝病毒e抗原及其e抗体(HBeAg、抗-Hbe)和乙肝病毒核心抗体(抗-HBc);根据定值参比血清和样本的HBsAg荧光速率值/阴性对照荧光速率值之比值(S/N值),并结合中和试验结果,确定浓度在5μg/L以下的HBsAg阳性例数,并分析相关乙肝病毒(HBV)血清标志物模式.结果 共检出HBsAg阳性816例,HBsAg浓度在5 μg/L以下的有189例,占总数的2.34%,占HBsAg阳性人群的23.16%,其中,HBsAg浓度在1 μg/L以下的有84例(44.04%);1~2 μg/L的有33例(17.5%);2~5 μg/L的有72例(38.10%).对上述低水平HBsAg人群的5项HBV血清标志物检测获得8种模式,以"HBsAg、抗-HBc、抗-Hbe阳性","HBsAg和抗-HBs阴性"模式为主(74.60%);累计HB-sAg和抗-HBc同时阳性者占94.17%;HBsAg与HBs同时阳性只出现在HBsAg 1 μg/L以下人群中(6.45%). 结论 低水平血清HBsAg人群不容忽视,提高HBsAg检测灵敏度有重要意义;同时检测相关HBV血清标志物,对于确定上述人群有帮助.

  7. 电化学发光法进行HBsAg阳性确认的可行性及应用研究%Feasibility and application of HBsAg positive confirmation by electrochemiluminescence assay in blood screening

    Institute of Scientific and Technical Information of China (English)

    邓雪莲; 陈辉; 王新梅; 臧亮; 王东; 高慧卉; 邹亚轩; 周璐; 梁晓华

    2016-01-01

    Objective To evaluate the feasibility of HBsAg positive confirmation by electrochemiluminescence assay (ECL) in blood screening and to investigate the gray area setting of HBsAg ELISA and an alternative method of HBsAg positive confirmation,and to simplify the algorithm of donor reentry.Method As the supplementary test,HBsAg ECL was adopted to retest samples of HBsAg reactive (ELISA) in blood screening and those HBsAg ECL reactive were confirmed by neutralization test (ECL).HBsAg positive was confirmed by combination of nucleic acid test (NAT),ECL and ELISA and blood donor follow-up.Neutralization test (ECL) and HBsAg (ECL) were compared for detecting HBV DNA positive among samples that were HBsAg reactive to both of two ELISA kits (S/CO≥ 1).The detection efficiency of HBsAg positive confirmed in different detection processes supplemented with HBsAg ECL were analyzed with sensitivity (SEN) and positive predictive value (PPV),as well as grey area setting.The S/CO value distributions of HBsAg false reactive and HBsAg positive were analyzed for each ELISA kit.Results Among 821 HBsAg reactive excluded from 192 065 samples collected in Dalian Blood Center between January 1st,2013 and June 30th,2015,about 160 HBsAg positive were confirmed by combination tests and blood donor follow-up.The ability of HBsAg (ECL) in detecting HBV DNA positive among samples that were HBsAg reactive to both of two ELISA kits (S/CO ≥ 1) was significandy higher than that of neutralization test (ECL) (P < 0.05).The PPV of different detection processes in detecting HBsAg positive confirmed was increased to 92.6%-99.2% by HBsAg (ECL) supplementary test (P =0).There was significant difference between S/CO value distributions of HBsAg false reactive and of the HBsAg positive confirmed for each ELISA kit.The plateau regions of SEN and PPV for two ELISA kits were observed when gray area setting was decreased.The SEN and PPV for ELISA1 were already in plateau regions when S/CO≤ 1,and S

  8. Prevalencia de anticuerpos contra Brucella sp. En donantes del banco de sangre de un hospital de Lima

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    Abel Ortega

    2007-10-01

    Full Text Available El Perú es un país endémico de Brucelosis, por lo que existe el riesgo de transmisión a través de sangre donada por no ser parte del tamizaje regular que realizan los bancos de sangre. Para conocer la prevalencia de anticuerpos contra Brucella en donantes del banco de sangre del Hospital Edgardo Rebagliati Martins, se analizó 1003 muestras de suero. La prueba tamiz fue Rosa de Bengala (RB; las muestras positivas fueron evaluadas por las pruebas de aglutinación en tubo (AT y 2-Mercaptoetanol (2-ME. Dos donantes fueron positivos a RB, confirmados por AT y 2-ME, se encontró una prevalencia de 0,20% (IC99%: 0,01-0,92. Se demostró la presencia de donantes portadores de anticuerpos contra Brucella con posible Brucelosis activa, evidenciándose la posibilidad de transmisión de esta infección. Se debe realizar mayores estudios con la finalidad de conocer la realidad de otros bancos de sangre.

  9. Anticuerpos monoclonales contra la Gonadotropina Corionica Humana (HCG y su empleo en el diagnóstico precoz del embarazo.

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    Torres E

    2006-03-01

    Full Text Available Se describe la obtención y caracterización de anticuerpos monoclonales contra la hormona gonadotropina cariónica humana (HCG, seleccionados de la inmunización subcutánea en ratones BALB/c. Se reporta también el desarrollo del sistema rápido HeberFast Line® Embarazo, para la detección de la hormona en muestras de orina, basado en los anticuerpos obtenidos y la tecnología del flujo lateral desarrollada en membranas de nitrocelulosa como soporte sólido. El nivel de sensibilidad alcanzado con este sistema diagnóstico es de 20 UI/L de HCG en muestra de orina, y no se observó reactividad cruzada en analitos similares conteniendo concentraciones de las glicohormonas relacionadas; 1000 IU de LH, 5000 IU de FSH, y 1000 µIU de TSH respectivamente. Los estudios de estabilidad arrojaron, que el diagnosticador mantine las características de funcionalidad por un tiempo de 15 meses a temperaturas entre 2 y 30 ºC, conformando este el tiempo de vigencia del sistema. De poseer en Farmacias, Consultorios Médicos de la Familia y Hospital Gineco-Obstétricos, un sistema rápido y sencillo como el establecido en este trabajo, se pueden evitar todos los riesgos e inconvenientes que acarrean los procesos de interrupción del embarazo en la mujer.

  10. Prevalencia de anticuerpos IgG al toxoide tetánico en trabajadores sanitarios

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    B. Pérez Bermúdez

    2014-09-01

    Full Text Available Introducción: En el año 2009 el Ministerio de Sanidad y Consumo de España publica una nueva guía para la vacunación de la Difteria y Tétanos. Existen discrepancias con los tiempos de inoculación de las dosis de recuerdo con otros países y sociedades médicas. Material y métodos: Estudio descriptivo. Las variables analizadas fueron: IgG-Toxoide Tetánico, tiempo última dosis de vacunación, dosis totales, edad, sexo, hospital, habito tabáquico e índice de masa corporal. Se construyó un modelo de Regresión Logística. Con el fin de determinar los puntos de corte más sensibles y específicos para las variables temporales (tiempo desde la última dosis y edad se calcularon las curvas R.O.C Resultados: Las variables, edad O,R= 17,56 (1,840 - 167,760, dosis de vacuna inoculadas al trabajador, O,R= 0,064 (0,006 - 0,908 y tiempo de la última dosis de vacunación, O,R= 1,03 (1,017 - 1,047, son las variables significativas en el análisis multivariante. Los puntos de cortes, para la edad son 54 años (Sensibilidad S= 0,72, Especificidad SP=0,77 y 15 años tras la última dosis años (S= 0,88, SP=0,86, por encima de este corte la probabilidad de no tener anticuerpos al Tétanos es alta. Conclusiones: Ante un trabajador que refiere o documenta que hace más de 15 años de la ultima dosis deberíamos plantearnos la revacunación, si además este trabajador refiere no tener más de 5 dosis inoculadas y tiene más de 54 años la revacunación sería muy recomendable.

  11. Comparison of three different recombinant hepatitis B vaccines: GeneVac-B, Engerix B and Shanvac B in high risk infants born to HBsAg positive mothers in India

    Institute of Scientific and Technical Information of China (English)

    Vijayakumar Velu; Subhadra Nandakumar; Saravanan Shanmugam; Suresh Sakharam Jadhav; Prasad Suryakant Kulkarni; Sadras Panchatcharam Thyagarajan

    2007-01-01

    AIM: To evaluate a low cost Indian recombinant hepatitis B vaccine GeneVac-B for its immunogenicity and safety in comparison to Engerix B and Shanvac B vaccine in high risk newborn infants born to (hepatitis B surface antigen) HBsAg positive mothers.METHODS: A total of 158 infants were enrolled in the study. Fifty eight infants were enrolled in the GeneVac-B group while 50 each were included for Engerix B and Shanvac B groups. A three-dose regimen of vaccination; at birth (within 24 h of birth), 1st mo and 6 mo. were adopted with 10 ng dosage administered uniformly in all the three groups. Clinical and immunological parameters were assessed for safety and immunogenicity of the vaccines, in all the enrolled infants.RESULTS: Successful follow up until seven months of age was achieved in 83% (48/58) for GeneVac-B, 76% (38/50) and 64% (32/50) for Engerix B and Shanvac B groups respectively. 100% seroconversion and seroprotection was achieved in all the three groups of infants. The geometric mean titers of anti-HBs one month after the completion of three dose of vaccination were 90.5, 80.9 and 72.5 mlU/mL in GeneVac-B, Engerix B and Shanvac B vaccine group respectively. Furthermore the level of anti-HBs increases with age of babies who were born to HBsAg positive mothers. The GMT values of anti-HBs were 226.7, 193.9 and 173.6 mlU/mL respectively in GeneVac-B, Engerix B and Shanvac B groups one year after the completion of the three doses of vaccine. No systemic reactions were reported in infants during the entire vaccination process of GeneVac-B and the other two vaccines. Clinical safety parameters remained within the normal limits throughout the study period.CONCLUSION: The study concludes that there is no significant difference between the three recombinant hepatitis B vaccines. Administration of these vaccines within 24 h of birth to babies, born to HBsAg positive mothers will reduce the incidence of HBV infection.

  12. Diagnóstico laboratorial de hemoglobinas semelhantes à HbS Laboratorial diagnosis for hemoglobin like HbS

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    Paula Juliana Antoniazzo Zamaro

    2002-01-01

    Full Text Available A hemoglobina S (HbS está presente na população brasileira com prevalência variável, dependente dos grupos raciais formadores de cada região. A migração eletroforética em pH alcalino apresenta similaridade com outras hemoglobinas, e estudos complementares para sua correta caracterização são necessários. No presente estudo objetivamos traçar um fluxograma com as metodologias disponíveis para a caracterização da hemoglobina S e das hemoglobinas que apresentam migração semelhante em pH alcalino. No período de janeiro a junho de 2000, analisamos amostras de sangue com suspeita de hemoglobina S encaminhadas ao Laboratório de Hemoglobinas da Unesp. Caracterizamos diferentes mutantes e formas interativas com hemoglobina S, por procedimentos eletroforéticos, em variados pH, análises citológicas e testes bioquímicos específicos. Os procedimentos de análise aplicados resultaram em orientação fornecida aos laboratórios de rotina sobre como proceder no diagnóstico laboratorial destas alterações de hemoglobina. Desta forma contribuímos para um melhor conhecimento sobre a variabilidade genética das hemoglobinas em nossa população, auxiliando no acompanhamento clínico e no aconselhamento genético das hemoglobinopatias com fisiopatologia relacionada à alteração.The hemoglobin S is presented at Brazilian population with variable prevalence dependent of constitutive racial groups in each region. The electrophoretic migration on alkaline pH showed similarities with another hemoglobins. Complementary studies to the correct caracterization becomes necessary. At the present study we aimed make a fluxogram with the avaliable methodologies for the caracterization of hemoglobin S and hemoglobins that present similar migration on alkaline pH. During the period of January to June, 2000, we had analised blood samples with suspicious hemoglobin S sent to the Laboratory of Hemoglobins, Unesp. Were caracterized different mutants and

  13. Aplicación de una biblioteca de anticuerpos lineales humanos frente al polisacárido capsular de Neisseria meningitidis serogrupo B

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    Emigdio León-Toirac

    2013-12-01

    Full Text Available Neisseria meningitidis serogrupo B produce aún morbimortalidad significativa. Su polisacárido capsular muestra similitud estructural con proteínas humanas y pobre inmunogenicidad, obstaculizando así el desarrollo de vacunas y anticuerpos monoclonales (AcM y policlonales contra esta bacteria. Recientemente se han creado bibliotecas artificiales de anticuerpos humanos expresados en bacteriófagos que reconocen específicamente a moléculas diana existentes, con la ventaja sobre los AcM convencionales por su rápida obtención, sin utilización de animales de laboratorio, lo que emerge como alternativa atractiva para la producción de AcM contra antígenos peculiares o complejos. Se realizó un trabajo de investigación básica, utilizando una biblioteca de fagos filamentosos que expresan constitutivamente regiones variables de anticuerpos humanos, que se enfrentó al polisacárido capsular de N. meningitidis serogrupo B. Los resultados que se obtuvieron mediante ELISA policlonal sugieren la existencia de anticuerpos humanos expresados en fagos que lo reconocen.

  14. Evaluación de anticuerpos contra enfermedades prevenibles por vacunas en el binomio madre–recién nacido en hospitales de Ciudad de La Habana

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    Rolando Felipe Ochoa

    2007-08-01

    Full Text Available El sistema inmune es inmaduro en los recién nacidos, lo que los hace susceptibles a las enfermedades infecciosas, sin embargo, pueden ser parcialmente protegidos por los anticuerpos adquiridos a través de la placenta. Por ello, nos propusimos evaluar la transferencia transplacentaria de anticuerpos contra algunas enfermedades prevenibles por vacunas. Se realizó un ensayo prospectivo, aleatorio, en el binomio madre–recién nacido. Para ello se analizó un total de 96 pares de muestras de suero, tomadas en el salón de preparto y al nacimiento en hospitales de Ciudad de La Habana, durante el año 2004. Se emplearon técnicas inmunoenzimáticas para determinar los niveles de antitoxina tetánica (ATT, antitoxina diftérica (ATD y anticuerpos contra proteínas de membrana externa del meningococo B (PME, contra el polisacárido capsular del meningococo C (PSC y contra el polisacárido Vi de Salmonella Typhi. Se detectó un nivel apropiado de ATT, tanto en las madres como en los recién nacidos, predominando los valores correspondientes a la protección de larga duración (>1 UI/mL, con 88,54% y 92,71%, respectivamente. Se observó niveles de ATD inferiores a 0,1 UI/mL, límite para una protección confiable, en el 54,17% y 45,83% de las madres y los recién nacidos. El 90,63% de las madres y el 96,88% de los recién nacidos presentaron concentraciones de anticuerpos contra PME superiores a 2 µg/mL, así como el 38,54% y el 41,67% para anticuerpos contra PSC. La seroprotección contra la Salmonella Typhi (>1 µg/mL fue de 5,21% y 8,33%, respectivamente. La mayoría de los recién nacidos presentaron títulos superiores de anticuerpos al de sus madres, lo cual demuestra que la transferencia transplacentaria de anticuerpos es un mecanismo eficiente.

  15. rhIL-12与HBsAg联合给药对食蟹猴rhIL-12药代动力学的影响%Effect of rhIL-12 combined with HBsAg on pharmacokinetics of rhIL-12 in Cynomolgus monkeys

    Institute of Scientific and Technical Information of China (English)

    李茹冰; 李健; 李淑穗; 陈新; 孙智平; 张宜俊; 傅泳航

    2012-01-01

    Objective To investigate the effect of rhIL-12 combined with HBsAg on pharmacokineties of rhIL-12 in Cynomolgus monkeys. Methods A double antibody sandwich BAS-ELISA method for serum rhIL-12 content in Cynomolgus monkeys was developed by using anti-human IL-12 monoclonal antibody as coating antibody and biotin-labeled anti-human IL-12 monoclonal antibody as detection antibody,and verified. Cynomolgus monkeys were divided into two groups,and injected with 90 μg/kg HBsAg + 0. 5 μg/kg rhIL-12 and 90 μg/kg HBsAg + 40 μg/kg rhIL-12 respectively. HBsAg was injected by i.m. route,while rhIL-12 by s.c. route. Serum samples were collected at various time points after injection and determined for rhIL-12 concentrations by the developed BAS-ELISA method,based on which the pharmacokinetic parameters were calculated by statistical moment method. Results The lower limit of quantificaion (LLOQ) of the developed BAS-ELISA method was 31. 25 pg / ml. The method showed high specificity,precision and accuracy,by which the determination results of samples treated under various conditions were stable. After injection with HBsAg + rhIL-12,the rhIL-12 concentrations in sera of Cynomolgus monkeys increased with the increasing dosage of rhlL-12 and reached peak values about 9 h after injection. The half-life of rhIL-12 in Cynomlgus monkeys after injection with 90 μg/kg HBsAg + 40 μg/kg rhIL-12 and 90 μg/kg HBsAg + 0. 5 μg/kg rhIL-12 were (21. 24 ± 2. 03) and (16. 05 ± 4. 76) h respectively. Conclusion The combination of rhIL-12 with HBsAg showed no effect on the pharmacokinetics of rhIL-12 in Cynomolgus monkeys,which laid foundation of clinical application of IL-12.%目的 探讨重组人白细胞介素-12(Recombinant human interleukin-12,rhIL-12)与乙型肝炎表面抗原(HBsAg)联合给药对食蟹猴rhIL-12药代动力学的影响.方法 以抗rhIL-12单克隆抗体为包被抗体,生物素标记的抗人IL-12单克隆抗体为检测抗体,建立双抗体夹心BAS-ELISA法,

  16. A Study on HBsAg Carrier Rate of Children under Age Five in Danzhou City

    Institute of Scientific and Technical Information of China (English)

    Bo Hu; Dui-Liu Li; Yuan-Gui Feng

    2015-01-01

    Objective:To learn the state of HBsAg carrier after implementing vaccine hepatitis B immunization program for children in Danzhou, and providing a scientific basis for the development of the hepatitis B vaccination strategies.Methods:A combination of stratified sampling and mechanical sampling method was carried out, the authors surveyed vaccine immunization history with hepatitis B in these children who were born from January 1, 2008 to December 31, 2012, then collected blood 3 mL and detected HBsAg carrier status by ELISA from these surveyed children.Results:250 children were included in the survey, the 24 h of birth Hepatitis B vaccine coverage rate in urban, rural newborns was 97.6% and 93.6%, respectively, hepatitis B vaccine three-pin vaccination rate for the whole vaccination course were 100%, only two children from rural area HBsAg carrier was positive, and their positive carrier rate was 0.8%, below 1%. Two HBsAg-positive carrier children from rural area and their families were under epidemiological survey, with discovery that their mothers were carriers of HBsAg, considered as mother to child transmission.Conclusions:Strengthen propaganda promotion to expand knowledge of Hepatitis B and to improve neonatal hepatitis B vaccine 24 h of the first dose timely rate, and standardize hepatitis B vaccine three-pin qualified vaccination rate for the whole vaccination course in children. Take effective measures to improve PMTCT rate, it can reduce child carrier rate of hepatitis B surface antigen, and take effective measures to improve PMTCT rates, it will reduce children carrier rate of HBsAg.

  17. Prevalencia de anticuerpos contra Leptospira en personas asintomáticos y en perros de Chancay, Lima 2001

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    Manuel Céspedes

    2007-10-01

    Full Text Available Objetivos. Determinar la prevalencia de anticuerpos contra Leptospiras en personas asintomáticas dedicadas a la agricultura, pesca y comercio y en perros domésticos de localidades de Chancay (Huaral, Lima. Material y métodos. Estudio transversal analítico, se tomó muestras de suero de 268 pobladores de tres localidades, en quienes se evaluó la presencia de anticuerpos totales contra Leptospiras por el método de ELISA IgG y la prueba de microaglutinación (MAT. Se buscaron los factores asociados con la positividad a Leptospiras calculando el OR con su intervalo de confianza al 95%. De la misma manera, se tomó muestras de suero de 241 perros a los que se realizó la prueba de MAT. Resultados. La prevalencia de anticuerpos contra Leptospira en población asintomática fue de 10,1% (IC95%: 6,3-13,9, la cual estuvo asociada con el abastecimiento de agua para consumo en quebrada o pozo (OR: 3,48, IC95%: 1,39-8,74, con el antecedente de nadar en el río o acequia (OR: 4,75, IC95%: 1,51-14,92 y con tener una edad entre 21-40 años (OR: 2,47, IC95%: 1,10-5,52. Los serovares más frecuentes fueron Icterohaemorrhagiae y Canicola según MAT. En canes, 27,8% (67/241 tuvieron serología positiva a leptospiras. Conclusiones. Existe una mediana prevalencia de serología positiva para Leptospiras en la población general asintomática y condiciones favorables para la presencia de Leptospiras en las localidades estudiadas. En estas zonas se recomienda realizar actividades educativas preventivas frecuentes, tomando en cuenta los resultados de este estudio y el personal de salud debe sospechar de la leptospirosis como una causa de enfermedad febril.

  18. 透析患者血清HBsAg假阳性伴HIV可疑一例分析%Analysis of a Case of Serum False-Positive HBsAg and Suspicious HIV Antibody in Dialysis Patient

    Institute of Scientific and Technical Information of China (English)

    吴卫; 许少侠; 黄媛; 郭野; 司永珍; 杨卓; 谢田; 王巧凤; 崔巍

    2012-01-01

    HBsAg和HIV抗体的检测分别在HBV感染和HIV感染的诊疗中具有重要意义.现阶段HBsAg和HIV抗体主要通过血清免疫学方法检测,其中ELISA法检测HBsAg或HIV抗体的假阳性病例时有报道,而全自动化学发光免疫分析仪的假阳性病例较为少见.该文即报道了一例用全自动化学发光免疫分析仪检测透析患者血清HBsAg假阳性伴HIV抗体可疑的病例,并对可能的原因进行了深入探讨.%HBsAg and HIV antibody play important role in the diagnosis and treatment of HBV and HIV infection respectively. At present, HBsAg and HIV antibody are mainly detected by serum immunological techniques. Cases of false-positive HBsAg or HIV antibody detected by ELISA were reported occasionally while false-positive cases by automatic chemilumi-nescence immune analyzers were reported rarely. A case of serum false-positive HBsAg and suspicious HIV antibody detected by automatic chemiluminescence immune analyzers in dialysis patient is reported in t paper and analyzed for the possible reasons.

  19. Modelamiento del sistema inmune aviar para la optimización de la producción de anticuerpos específicos / Modelling of the avian immune system for the optimization of specific antibody production

    OpenAIRE

    Olarte Dussan, Fredy Andres

    2011-01-01

    Esta tesis presenta una metodología para optimizar la producción de anticuerpos específicos en aves. Anticuerpos (también conocidos como inmunoglobulinas) son herramientas biotecnológicas de gran valor usadas en investigación, diagnóstico y tratamiento. Usualmente, la producción de anticuerpos se realiza por medio de la inoculación en varias oportunidades de un animal con una concentración particular de un antígeno y la recolección de estas moléculas en las respuestas secundarias ...

  20. Construction and immunological evaluation of truncated hepatitis B core particles carrying HBsAg amino acids 119–152 in the major immunodominant region (MIR)

    Energy Technology Data Exchange (ETDEWEB)

    Su, Qiudong; Yi, Yao [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China); Guo, Minzhuo [Beijing Entry-Exit Inspection and Quarantine Beureau, Tianshuiyuan Lane 6, Chaoyang District, Beijing 100026 (China); Qiu, Feng; Jia, Zhiyuan; Lu, Xuexin; Meng, Qingling [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China); Bi, Shengli, E-mail: shengli_bi@163.com [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China)

    2013-09-13

    Highlights: •The conformational HBV neutralization antigen domain was successfully displayed on the surface of truncated HBc particles. •Appropriate dialysis procedures to support the renaturing environment for the protein refolding. •Efficient purification procedures to obtain high purity and icosahedral particles of mosaic HBV antigen. •Strong immune responses not only including neutralization antibody response but also Th1 cell response were induced in mice. -- Abstract: Hepatitis B capsid protein expressed in Escherichia coli can reassemble into icosahedral particles, which could strongly enhance the immunogenicity of foreign epitopes, especially those inserted into its major immunodominant region. Herein, we inserted the entire ‘α’ antigenic determinant amino acids (aa) 119–152 of HBsAg into the truncated HBc (aa 1–144), between Asp{sup 78} and Pro{sup 79}. Prokaryotic expression showed that the mosaic HBc was mainly in the form of inclusion bodies. After denaturation with urea, it was dialyzed progressively for protein renaturation. We observed that before and after renaturation, mosaic HBc was antigenic as determined by HBsAg ELISA and a lot of viruslike particles were observed after renaturation. Thus, we further purified the mosaic viruslike particles by (NH{sub 4}){sub 2}SO{sub 4} precipitation, DEAE chromatography, and Sepharose 4FF chromatography. Negative staining electron microscopy demonstrated the morphology of the viruslike particles. Immunization of Balb/c mice with mosaic particles induced the production of anti-HBs antibody and Th1 cell immune response supported by ELISPOT and CD4/CD8 proportions assay. In conclusion, we constructed mosaic hepatitis core particles displaying the entire ‘α’ antigenic determinant on the surface and laid a foundation for researching therapeutic hepatits B vaccines.

  1. OBTENCIÓN DE ANTICUERPOS ESPECÍFICOS PARA LA DETECCIÓN DEL Tamarillo leaf malformation virus (TALMV EN TOMATE DE ÁRBOL Specific Antibodies to Detect Tamarillo leaf malformation virus (TaLMV in Tamarillo

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    YULIANA GALLO GARCÍA

    2011-08-01

    Full Text Available En Colombia el rendimiento del cultivo de tomate de árbol se ha visto seriamente afectado por la expansión de una enfermedad conocida como virosis de tomate de árbol. Esta patología se registró inicialmente en 1991 en el norte de Antioquia y su expansión ha alcanzado todas las regiones cultivadoras de este frutal en el país. Trabajos recientes han detectado la presencia de por lo menos dos especies del género Potyvirus (Potyviridae asociadas a esta enfermedad en los cultivos de tomate de árbol de Antioquia: Potato virus Y (PVY y Tamarillo leaf malformation virus (TaLMV, especie propuesta. Con el fin de reducir la diseminación de estos patógenos virales en el país, es necesario contar con herramientas de diagnóstico que permitan la certificación del material de siembra y la detección temprana en plantas asintomáticas. En este trabajo se obtuvieron anticuerpos policlonales específicos para la detección del virus TaLMV utilizando una región antigénica de 15 residuos de la cápside viral. La sensibilidad y especificidad de los anticuerpos anti-TaLMV fue evaluada mediante pruebas de ELISA y dot-blot utilizando proteína recombinante y péptidos sintéticos como controles. La utilidad de estos anticuerpos fue validada a partir de una prueba piloto de detección de TaLMV en muestras de plantas de tomate de árbol con y sin síntomas de virosis obtenidas en el oriente antioqueño. Los resultados serológicos fueron comparados con los niveles de detección que ofrece la técnica de RT-PCR con cebadores específicos para la cápside viral de TaLMV.In Colombia, yields of tamarillo are seriously affected by a complex viral disease known as virosis. This pathology was first reported in 1991 in the north of Antioquia and currently affects all tamarillo growing regions in the country. Recent works have demonstrated the association of two potyviruses (potyviridae with this disease: Potato virus Y (PVY and Tamarillo leaf malformation virus

  2. Seroprevalencia de anticuerpos contra Moquillo canino en perros no vacunados en dos municipios de Ciudad de la Habana

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    Márquez Álvarez, Mario:

    2006-09-01

    Full Text Available Esto es el resumen de un trabajorealizado con el objetivo de determinarla presencia de anticuerpos séricoscontra Moquillo Canino en perros novacunados de dos municipios de Ciudadde la Habana, se estudiaron 117 canesmayores de 1 año de edad y con accesoa la calle, 51 del municipio Boyeros y 57del municipio Cotorro, que no contabancon antecedentes de vacunaciónanterior. Se estudiaron además 9animales vacunados recientemente quese utilizaron como controles positivos delestudio. Se tomaron datos referentes ala edad y sexo de los animales y seextrajeron muestras de sangre quefueron centrifugadas para la obtenciónde suero. Para la determinación deanticuerpos se empleó un ELISAestandarizado por la División deBiotecnología y Control de la Calidad delCentro Nacional para la Producción deAnimales de Laboratorio (Cuba. Lasplacas se leyeron en un lector SensidentScan a 414 nm. Se consideró como valorde corte el valor promedio de laDensidad Óptica del suero controlnegativo. La actividad de anticuerpos delas muestras se midió sobre la base delvalor de Densidad Óptica obtenidoconsiderando positivas todas las quepresentaron valores por encima del Valorde Corte. Se establecieron rangosagrupando los valores individuales enaltos, medios y bajos. Se comprobó queexiste una alta seroprevalencia deanticuerpos contra el virus en losmunicipios estudiados, con valores deDensidad Óptica en su mayoría incluidosdentro de los rangos medio y alto, lo queevidencia una amplia circulación delagente en el área, sin que se observarainfluencia del sexo, la edad de losanimales y el municipio de procedenciasobre la respuesta inmune observada.

  3. ANTICUERPOS DIRIGIDOS A PEQUEÑAS MOLÉCULAS DE NEUROTRANSMISORES Y PÉPTIDOS: PRODUCCIÓN Y APLICACIÓN DE ANTICUERPOS A DOPAMINA, SEROTONINA, GABA, VASOPRESINA, PÉPTIDO VASOACTIVO INTESTINAL, NEUROPÉPTIDO Y, SOMATOSTATINA Y SUSTANCIA P

    OpenAIRE

    Buijs, Ruud M.; Ma. del Carmen Basualdo S.

    2009-01-01

    En el presente trabajo se describe un procedimiento por medio del cual pueden ser obtenidos anticuerpos a partir de moléculas pequeñas de neurotransmisores usando glutaraldehido como agente acoplador. Este procedimiento fue desarrollado bajo la condición de que el inmunógeno usado para la preparación del anticuerpo fuera, hasta donde fuese posible, de la misma identidad que la molécula transmisora fijada al tejido. Con el de fin de lograr mayor éxito, los péptidos, aminas o aminoácidos1 fuero...

  4. Co-administration of CpG oligonucleotides enhances the late affinity maturation process of human anti-hepatitis B vaccine response.

    Science.gov (United States)

    Siegrist, Claire-Anne; Pihlgren, Maria; Tougne, Chantal; Efler, Sue M; Morris, Mary Lou; AlAdhami, Mohammed J; Cameron, D William; Cooper, Curtis L; Heathcote, Jenny; Davis, Heather L; Lambert, Paul-Henri

    2004-12-16

    We assessed the avidity maturation process elicited by human immunization with alum-adsorbed HBsAg alone or with a novel adjuvant containing CpG motifs (CpG 7909). Mean avidity indexes and distribution of low- and high-avidity anti-HBs indicated that avidity maturation essentially takes place late after priming. CpG 7909 markedly enhanced this affinity maturation process, increasing the pool of high-avidity antibodies. The influence of CpG 7909 was antigen-specific, isotype-specific and distinct from the influence on anti-HBs production, as avidity did not correlate with anti-HBs IgG titers. This is the first demonstration that a novel human adjuvant may induce antibodies with higher antigen-binding affinity.

  5. Quantitation of HBsAg predicts response to entecavir therapy in HBV genotype C patients

    Institute of Scientific and Technical Information of China (English)

    Etsuro Orito; Kei Fujiwara; Hiroshi Kanie; Tesshin Ban; Tomonori Yamada; Katsumi Hayashi

    2012-01-01

    AIM:To analysis the factors that predict the response to entecavir therapy in chronic hepatitis patients with hepatitis B virus (HBV) genotype C.METHODS:Fifty patients [hepatitis B e antigen (HBeAg)-negative:HBeAg-positive =26:24] with HBV genotype C,who received na(i)ve entecavir therapy for > 2 years,were analyzed.Patients who showed HBV DNA levels ≥3.0 log viral copies/mL after 2 years of entecavir therapy were designated as slow-responders,while those that showed < 3.0 log copies/mL were termed rapidresponders.Quantitative hepatitis B surface antigen (HBsAg) levels (qHBsAg) were determined by the Architect HBsAg QT immunoassay.Hepatitis B core-related antigen was detected by enzyme immunoassay.Pre-C and Core promoter mutations were determined using by polymerase chain reaction (PCR).Drug-resistance mutations were detected by the PCR-Invader method.RESULTS:At year 2,HBV DNA levels in all patients in the HBeAg-negative group were < 3.0 log copies/mL.In contrast,in the HBeAg-positive group,41.7% were slow-responders,while 58.3% were rapid-responders.No entecavir-resistant mutants were detected in the slow-responders.When the pretreatment factors were compared between the slow-and rapid-responders;the median qHBsAg in the slow-responders was 4.57log IU/mL,compared with 3.63 log IU/mL in the rapidresponders (P < 0.01).When the pretreatment factors predictive of HBV DNA-negative status at year 2 in all 50 patients were analyzed,HBeAg-negative status,low HBV DNA levels,and low qHBsAg levels were significant (P < 0.01).Multivariate analysis revealed that the low qHBsAg level was the most significant predictive factor (P =0.03).CONCLUSION:Quantitation of HBsAg could be a useful indicator to predict response to entecavir therapy.

  6. Frekuensi HBsAg Positif pada Uji Saring Darah di Palang Merah Indonesia Cabang Padang Tahun 2012

    Directory of Open Access Journals (Sweden)

    Nadia Ventiani

    2015-09-01

    Full Text Available Abstrak Infeksi virus hepatitis B dapat berkembang menjadi hepatitis kronis, sirosis hati, kanker hati dan bahkan kematian. Salah satu cara penularannya adalah melalui transfusi darah. Tujuan penelitian ini adalah untuk mengetahuifrekuensi HBsAg positif pada uji saring darah donor.  Telah dilakukan penelitian deskriptif dengan menggunakan datasekunder di PMI Padang dengan 26.975 darah donor yang diteliti. Donor laki-laki lebih banyak dari donor perempuandengan perbandingan 11,69:1, dan pendonor sukarela lebih banyak dari pendonor pengganti, dengan perbandingan2,95:1. Pendonor terbanyak terdapat pada kelompok usia dibawah 30 tahun, yaitu sebanyak 38,09%. Hasil penelitianmenunjukan persentase darah donor dengan HBsAg positif sebesar 3,61%. Pendonor laki-laki dengan HBsAg positifsebesar 93,22%, perempuan 6,78% dan pendonor sukarela sebesar 65,09%, pendonor pengganti sebesar 34,91%.Hasil HBsAg positif terbanyak terdapat pada kelompok usia dibawah 30 tahun sebesar 39,01%. Sebagian besar darahdonor yang mengandung HBsAg positif terdapat pada kelompok umur di bawah 30 tahun. Frekuensi HBsAg positif lebih banyak pada donor laki-laki dibanding donor perempuan, dan donor sukarela dengan HBsAg positif lebih banyak dibanding donor pengganti.Kata kunci: HBsAg, donor darah, transfusi darahAbstract Hepatitis B virus infection could progress into chronic hepatitis, liver cirrhosis, liver cancer and even death. One mode of transmission is via blood transfusion. The objective of this study was to determine the frequency of positive HBsAg in the screening test of the blood donors. A descriptive studies has been conducted by using secondary datasin  PMI Padang. There were 26975 blood donors studied that  men donors were higher than female donors, with the number ratio of male and female were 11.69:1, and the number of voluntary donors were higher compared to the replacement donors, with ratio 2.95:1. Most of the donors were in the age group below 30 years, which

  7. Hepatitis B Virus Infection and Anti-HBc (Total Positivity in CKD Patients before Dialysis

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    Fareha Jesmin Rabbi

    2016-09-01

    Full Text Available Background: CKD patients are associated with HBV infection both as a cause and complication of treatment. CKD patients before starting dialysis therapy are considered as a high risk group because of impaired immune response compared with healthy individuals and also other risk factors related with treatment and management. Only HBsAg marker does not always follow the presence or absence of HBV infection. Anti-HBc (total alone positivity indicates previous exposure to HBV infection, window period and even after reactivation of resolved HBV infection. In some cases only anti-HBc positivity is interpreted as possible chronic low dose HBV infection (chronic carriage. Predialytic CKD patients were tested with three serological markers [HBsAg, anti-HBc (total and anti-HBs] for screening HBV infection. Proper diagnosis before dialysis and knowing the infection status would help both the patient and doctor to choose proper treatment approach. Objective: This cross-sectional study was done in the CKD patients before starting dialysis therapy to find out the HBV infection and to evaluate the infection by minimal serological markers as for screening. Materials and Methods: A total of 211 patients with chronic kidney disease stage five (CKD-V before starting dialysis therapy were included as subjects of this cross-sectional study. Among the CKD patients HBsAg was tested to see the prevalence. Other serological markers, i.e., anti-HBc (total and anti-HBs were tested in combination with HBsAg in 89 randomly selected patients among the subjects. The patients were also tested for anti-HCV to assess co-infection. After collecting all the data of different test results analyses were done by SPSS version 15.0. Results: Among total study population 10 (4.7% patients were found HBsAg positive. No patient was found positive for both HBsAg and anti-HCV. Among the 89 CKD patients only 2 (2.2% patients were HBsAg positive, and only one patient (0.9% was found positive

  8. Obtención de un anticuerpo contra la proteína p65 del virus de la tristeza de los cítricos y resultados preliminares de la expresión in vivo Obtaining a citric tristeza virus p65 protein antibody and preliminary results of p65 in vivo expression

    Directory of Open Access Journals (Sweden)

    Acosta Orlando

    2003-12-01

    Full Text Available El virus de la tristeza de los cítricos (CTV pertenece a la familia Closteroviridae que incluye a los únicos virus vegetales que poseen en su genoma genes homólogos a las proteínas de choque térmico celulares HSP70. Recien­temente se ha demostrado que las proteínas virales HSP70h, como la p65 de CTV, realizan funciones para el ensam­blaje del virión, como proteína de unión a microtúbulos y de movimiento célula a célula. De allí la importancia de desarrollar trabajos relacionados con esta proteína en el CTV, considerado el patógeno más deletéreo para los cítricos. En el presente estudio, fueron utilizados cuatro péptidos sintéticos correspondientes a la región carboxi-terminal de la proteína de choque térmico p65 del virus de la tristeza de los cítricos (CTV, para inmunizar conejos con el objetivo de obtener anticuerpos policlonales. Todos los péptidos utilizados fueron inmunogénicos. Ninguno de los anticuerpos obtenidos reaccionó contra el extracto de plantas no infectadas pero sí detectaron las proteínas p65 presentes en los extractos proteicos de cítricos infectados con el CTV. Se demostró un comportamiento inmunológico del anticuerpo anti p65 que parece ser independiente de la severidad sintomática del aislado de CTV utilizado, sobre la base de la exposición del anticuerpo a dos aislados colombianos con características serológicas diferentes. Se demostró que el ORF que codifica en el CTV para el homólogo de la HSP70 se expresa in vivo aunque el anticuerpo anti p65 solamente detectó extractos proteicos concentrados de plantas infectadas, lo que apoya lo informado en otros estudios sobre la baja concentración de esta proteína en plantas infectadas con el CTV. Es la primera vez que se obtiene en Colombia un anticuerpo policlonal anti CTV y, particularmente, contra p65, proteína que interviene en el ensamblaje y movimiento viral. La adaptación de una técnica de obtención de anticuerpos anti p65 a

  9. Tuberculosis meníngea: desarrollo de inmunoensayos para detección de anticuerpos específicos y su potencial uso diagnóstico

    Directory of Open Access Journals (Sweden)

    María L. Gennaro

    2001-04-01

    Full Text Available

    En el mundo y en Colombia la tuberculosis meníngea (TBM es la causa más frecuente de las meningitis crónicas (1,2. Las características clínicas y los hallazgos de laboratorio la sugieren pero no la confirman, requiriéndose el cultivo que es demorado y de baja sensibilidad (2. La detección de anticuerpos contra una combinación de antígenos podría contribuir al diagnóstico (3.
    Para evaluarlo, nuestro objetivo fue determinar la utilidad diagnóstica de la detección de anticuerpos específicos para Mycobacterium tuberculosis en líquido cefalorraquídeo (LCR.

     

     

  10. Status of HBsAg seroprevalence in 15 million rural couples in China: a cross-sectional study

    Science.gov (United States)

    Zhang, Long; Wang, Yuan-Yuan; Huang, Yan-Jie; Wang, Qiao-Mei; Nelson, Kenrad E.; Wang, An-Qi; Shen, Hai-Ping; Liu, Xiao-Li; Zhang, Yi-Ping; Yan, Dong-Hai; Peng, Zuo-Qi; Zhang, Hong-Guang; Zhang, Ya; Zhao, Jun; Wang, Yan; Yang, Ying; He, Yuan; Xu, Ji-Hong; Liu, Du-Jia; Guo, Tong-Jun; Xin, Xiao-Na; Zhou, Hong; Ma, Xu

    2017-01-01

    A cross-sectional analysis of prevalence of hepatitis B virus infection (HBV) among rural couples was conducted between 2010 and 2014. Serologic HBV markers, including hepatitis B surface antigen (HBsAg) and e antigen (HBeAg), were tested. Primary outcome of interest comprised HBsAg positivity in couples (both positive: F+M+, only wife positive: F+M−, only husband positive: F−M+), and secondary outcome consisted of prevalence and risk factors of HBsAg positivity among husbands or wives. Of 14,816,300 couples included, 0.7% were F+M+; 6.3% were F−M+; 4.4% were F+M−, resulting in the overall seroprevalence of 11.4%. Individually, 6.1% were HBsAg positive with a higher rate seen in husbands (7.0%) than in wives (5.2%). Wife’s HBeAg(+)/HBsAg (+) (AOR = 2.61), HBeAg(−)/HBsAg (+) (AOR = 2.23), positivity of syphilis (AOR = 1.50), living in a high-risk region (AOR = 1.46) were significantly predictors of HBsAg positivity in husbands. Prevalence and predictors of HBsAg positivity in wives had similar results. Our data show a high burden and discordant pattern of HBV infection in rural couples, and partner’s double positivity of HBeAg and HBsAg was the most significant factor of HBV infection in couples. A comprehensive strategy that emphasizes vaccination and education is needed. PMID:28220812

  11. The Prevalence of Hepatitis B Virus Surface Antigen (HBsAg Variations and Correlation with the Clinical and Serologic Pictures in Chronic Carriers from Khorasan Province, North-East of Iran

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    Alireza Namazi

    2012-04-01

    Full Text Available This study was designed to determine the correlation of hepatitis B virus surface Ag (HBsAg variations with the clinical/serological pictures among chronic HBsAg positive patients. The surface gene (S-gene was amplified and directly sequenced in twenty-five patients. Eight samples (group I contained at least one mutation at the amino acid level. Five showed alanine aminotransferase (ALT levels above the normal range of which only one sample was anti-HBe positive. Group II (17 samples did not contain any mutation, 4 were anti-HBe positive and 9 had increased ALT levels. In both groups, from a total of 18 mutations, 5 (27.5% and 13 (72.5% occurred in anti-HBe and HBeAg positive groups respectively. The small number of amino acid mutations might belong to either the initial phase of chronicity in our patients; or that even in anti-HBe positive phase in Iranian genotype D-infected patients, a somehow tolerant pattern due to the host genetic factors may be responsible.

  12. Anticuerpos frente a virus West nile y otros virus transmitidos por artropodos en la poblacion del Delta del Ebro

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    Lozano Alvaro

    1998-01-01

    Full Text Available FUNDAMENTOS: El virus West Nile (VWN es un Flavivirus que se transmite al hombre a través de distintas especies de mosquitos y produce brotes y casos esporádicos de enfermedad en distintas regiones del Viejo Mundo, incluída la Cuenca Mediterránea. Las zonas húmedas europeas que acogen aves migratorias procedentes de África constituyen áreas de alto riesgo para esta infección, así como para otras infecciones víricas transmitidas por artrópodos. MÉTODOS: Con objeto de investigar la prevalencia de la infección por el VWN y otros virus de transmisión similar en la población humana del Delta del Ebro, se estudiaron 1037 muestras de suero, obtenidas en 10 localidades de la zona, para presencia de anticuerpos frente a VWN y otros 12 virus transmitidos por artrópodos (3 Alfavirus, 8 Flaviviridae y 1 Bunyaviridae mediante titulación por inhibición de la hemaglutinación (IHA. En algunos casos se estudió la presencia de IgM específica por IHA tras fraccionar el suero por centrifugación en gradientes de sacarosa. RESULTADOS: En total, se encontró reactividad significativa frente a alguno de los virus probados en 130 casos (12.5%; 4.1% frente a Alfavirus, 8.0% frente a Flaviviridae y 0.4% frente a Bunyaviridae. El análisis de los títulos de anticuerpos reveló porcentajes significativos de muestras con títulos elevados frente a antígenos de VWN y otros. La distribución de la seroprevalencia fue muy desigual, concentrándose fundamentalmente en 3 localidades del interior del Delta (Ampolla, San Jaime y Montells, donde la prevalencia de anticuerpos frente a Flaviviridae llegó a alcanzar el 30% y se observaron niveles residuales de IgM frente a VWN en algunos sueros. CONCLUSIONES: Estos resultados y los obtenidos previamente en otras regiones de la Península Ibérica sugieren que el VWN circula en la población humana de las zonas de riesgo y produce brotes epidémicos periódicos. Habida cuenta del alto porcentaje de

  13. Anticuerpos antifosfolípidos en nefropatía membranosa idiopática

    OpenAIRE

    Ardiles A,Leopoldo; Blackburn T,Eileen; Alruiz S,Paula; Flores W,Claudio; Mezzano A,Sergio

    2005-01-01

    Background: Antiphospholipid antibodies have been found in the sera from patients with idiopathic and secondary glomerulopathies, mainly related to lupus. No special attention has been devoted to idiopathic membranous nephropathy, a glomerular disease with a high frequency of thrombotic complications, particularly of the renal vein. Aim: To study the presence and significance of antiphospholipid antibodies in idiopathic membranous nephropathy. Material and methods: Anticardiolipin and anti-ß2...

  14. The biological meaning of anti-HBC positive result in blood donors: relation to HBV-DNA and to other serological markers Significado biológico do resultado anti-HBc positivo em doadores de sangue: relação com HBV-DNA e outros marcadores sorológicos

    Directory of Open Access Journals (Sweden)

    Luiz C. Arraes

    2003-06-01

    Full Text Available In order to assess the potential risk of anti-HBc-positive blood donors for post-transfusional hepatitis and to investigate whether other HBV serological markers are capable of identifying the presence of the virus, 1000 first-time blood donors were enrolled between June and July 1997. These donors were screened using routine Brazilian blood center tests (HIV 1 and 2, HTLV 1 and 2, Chagas disease, Syphilis, HCV, HBsAg, anti-HBc and ALT . The 120 (12% found to be anti-HBc-positive underwent further tests: HBe, anti-HBe, anti-HBs and HBV-DNA by PCR. Ten cases were HBsAg positive and all were HBV-DNA positive by PCR. Three HBsAg-negative donors were HBV-DNA-positive. Two HBV-DNA-positive donors were also anti-HBs-positive. All the HBV-positive donors had at least one HBV marker other than anti-HBc. Anti-HBc is an important cause of blood rejection. Testing for HBsAg alone is not fully protective and anti-HBc remains necessary as a screening test. The presence of anti-HBs is not always indicative of absence of the virus. The addition of other HBV serological markers could represent an alternative in predicting the presence of the virus when compared with PCR. It is recommended that other studies should be carried out to confirm this finding.Para verificar o risco potencial para hepatite viral pós-transfusional de doadores anti-HBc positivos e investigar se outros marcadores sorológicos do HBV poderiam identificar a presença ou não do vírus, mil doadores de primeira vez foram recrutados entre junho e julho de 1997. Estes doadores foram testados para os testes de rotina utilizados em centros de transfusão brasileiros. Cento e vinte desses doadores foram anti-HBc positivos (12%. Nestes foram realizados os testes HbeAg, anti-HBc, anti-HBs e a pesquisa do HBV-DNA por PCR. Dez eram HbsAg positivos, todos com presença do HBV-DNA demonstrada por PCR. Três doadores HbsAg negativos foram HBV-DNA positivos. Dois doadores HBV-DNA positivos também o

  15. Increased susceptibility to experimental steatohepatitis induced by methionine-choline deficiency in HBs-Tg mice

    Institute of Scientific and Technical Information of China (English)

    Miao-MiaoFu; RuiSun; Zhi-GangTian; Hai-MingWei

    2010-01-01

    BACKGROUND: Worldwide, about 25% of individuals with chronic hepatitis B have fatty liver disease. Lipogenic diets that are completely devoid of methionine and choline induce nonalcoholic fatty liver disease. However, no animal model of nonalcoholic steatohepatitis associated with HBV infection is available, and the influence of viral infection on nutritional hepatic steatosis is unclear. METHODS: We used HBV surface antigen transgenic mice (HBs-Tg mice), which mimic healthy human carriers with hepatitis B surface antigen. The mice were fed with a high-fat methionine-choline-deficient diet (MCD) to build a reliable rodent nutritional model of nonalcoholic steatohepatitis associated with HBV infection, and the changes in body weight and serum triglycerides were measured. Hepatocyte ballooning changes were determined by hematoxylin and eosin staining. The extent of hepatic fat accumulation was evaluated by oil red O staining. Immunohistochemical assays were performed to detect proliferating cell nuclear antigen as an index of cell proliferation. RESULTS: MCD feeding provoked systemic weight loss and liver injury. MCD feeding caused more macrovesicular fat droplets and fat accumulation in the livers of HBs-Tg mice than in wild-type C57BL/6 mice. In addition, within 30 days of MCD exposure, more PCNA-positive nuclei were found in the livers of HBs-Tg mice. CONCLUSIONS: HBs-Tg mice fed with a lipogenic MCD form more macrovesicular fat droplets earlier, coincident with more hepatocyte proliferation, resulting in the appearance of increased susceptibility to experimental steatohepatitis in these mice.

  16. Prevalencia de marcadores serológicos para hepatitis B y delta e infección intrafamiliar en el valle del río Pampas, Perú¹

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    Gualberto Segovia M

    2002-04-01

    Full Text Available Objetivo: Determinar la prevalencia de marcadores serológicos para hepatitis viral B (HBV y Delta (HDV en la población escolar de las localidades del valle del Río Pampas y evaluar el riesgo de transmisión intrafamiliar de HBV. Materiales y métodos: esta investigación fue realizada en 8 localidades del valle del Río Pampas (Ayacucho-Andahuaylas de marzo a noviembre de 2000 y estuvo compuesta por dos fases: en la primera fase (seroprevalencia, se seleccionó una muestra en forma aleatoria y estratificada de 130 del total de escolares registrados, quienes fueron evaluados para identificar la presencia de HBsAg, anticuerpos anti-HBcAg y anti-HDV (sólo a los HBsAg positivos. En la segunda fase (riesgo de transmisión intrafamiliar, se anidó un estudio caso-control, en el cual los casos fueron los familiares de los niños reactivos al HBsAg y los controles fueron los familiares de los escolares no reactivos (relación 1/1. En todos se evaluó la presencia de anticuerpos anti-HBcAg, HBsAg y HbeAg. Resultados: 18 (13,8% escolares resultaron ser portadores de HBsAg, 93 (71,5% tuvieron anticuerpos anti-HBcAg y 3 (16,7% de 18 portadores de HBsAg tuvieron anticuerpos anti-HDV. No se encontró diferencias significativas en los marcadores serológicos de HBV entre los casos y controles. Conclusión: las localidades del valle del río Pampas (Ayacucho-Andahuaylas, presentan una alta endemicidad para el HBV y una endemicidad intermedia para el HDV. No se halló riesgo de transmisión intrafamiliar.

  17. 胶体金法与酶联免疫法联合检测HBsAg的结果比较%Compared results of colloidal gold method and enzyme linked immunosorbent assay in HBsAg

    Institute of Scientific and Technical Information of China (English)

    张晓飞

    2013-01-01

    目的 探讨基层医院HBsAg的有效检测方法.方法 采用胶体金法(GIA)与酶联免疫法(EIA)对18 638例血清标本同时进行HBsAg检测,并且对结果进行对比分析.结果 18 638例标本中有1例HBsAg EIA阴性而GIA阳性;13例HBsAg EIA阳性或OD值为临界值的样本,GIA结果为阴性;其余的标本EIA和GIA对于HBsAg阴性及阳性的标本检测结果一致.结论 两种方法对HBsAg检测的特异性相近.两种方法若联合检测应用于临床,可提高检测结果的准确性.

  18. Evaluation of cellular responses for a chimeric HBsAg-HCV core DNA vaccine in BALB/c mice

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    Maryam Yazdanian

    2015-01-01

    Conclusion: Fusion of HBsAg to HCVcp in the context of a DNA vaccine modality could augment Th1-oriented cellular and CTL responses toward a protective epitope, comparable to that of HCVcp (subunit HCV vaccine immunization.

  19. HBsAg detection by passive hemagglutination (Hepanosticon--Organon). Advantages and disadvantages in comparison with other methods.

    Science.gov (United States)

    Balş, M G; Hagiescu, L

    1976-01-01

    Investigated comparatively with immunodiffusion, electroimmunodiffusion, complement fixation and Latex agglutination, passive hemagglutination with the Hepanosticon--Organon reagent proved to be an easy, rapid, highly reproducible method for HBsAg detection.

  20. Prevalence of HBsAg Positive among Afghan Sweepers in Tehran, 2009

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    Seyed Mohammad Javad Hosseini

    2013-07-01

    Full Text Available AbstractBackground and objective: Hepatitis B virus is one of the great public health problems all over the world. Moreover prevalence of Hepatitis B in Iran is near 1.5 million (2.03% of general population. The objective of this study was to determine the prevalence of Hepatitis B among Afghan sweepers in 2, 5 and 9 municipal areas of Tehran in 2009.Materials and methods: This was a cross-sectional study on 250 afghan sweeper in 2, 5 and 9 municipal areas of Tehran. Demographic form including age, duration of working and migration, history of sexual contacts, needle stick injury, IV drug abuse, surgery and blood transfusion was completed for all cases. The blood samples were collected and examined for HBsAg by Eliza test at Iran Blood transfusion organization laboratory.Results: From 250 Afghan sweepers enrolled in this study, HBsAg positive was detected in 10 (4% cases. We found significant relation between the HBsAg positive, needle stick injury, unprotected sexual contact, age and time migration to Iran (P<0.05.Conclusion: Prevalence of Hepatitis B was approximately high among afghan sweeper in Tehran. The results indicated that prevalence of risk factors like needle stick injury and unprotected sexual contact were high among Afghan sweepers. Educations of Hepatitis B, ways of transmission, screening of infectious disease, and other blood-born infection to sweepers are important.

  1. Detection of HBsAg, HBcAg, and HBV DNA in ovarian tissues from patients with HBV infection

    Institute of Scientific and Technical Information of China (English)

    Li-Zhang Chen; Xue-Gong Fan; Jian-Ming Gao

    2005-01-01

    AIM: To investigate the presence of HBsAg, HBcAg, and HBV DNA in ovarian tissues from patients with HBV infection.METHODS: HBsAg and HBcAg were examined in ovarian biopsy tissues from 26 patients with HBV infection by immunocytochemistry, and HBV DNA was detected in ovarian tissues by PCR.RESULTS: HBsAg and HBcAg were present with the same positive rate of 34.6% (9/26). The total positive rate was 46.2% (12/26). HBsAg and HBcAg were positive in 6 (23.1%) of the 26 patients. Brown positive particles were diffusely distributed in ovarian cells. The positive rate of HBV DNA was 58.3% (7/12).CONCLUSION: HBsAg, HBcAg, and HBV DNA can be detected in ovarian tissues from patients with HBV infection. The presence of HBsAg and HBcAg in ovarian tissues does not correlate with the HBV markers in serum.

  2. Enzyme-linked Immunoassay (ELISA) for Testing and Evaluation of Low Concentration of HBsAg%酶联免疫法(ELISA)对低浓度HBsAg的检测与评价

    Institute of Scientific and Technical Information of China (English)

    韩福红

    2015-01-01

    目的:探讨酶联免疫法(ELISA)在低浓度HBsAg检测中的效果。方法以本院2014年1~12月门诊与住院患者中53例采用科华ELISA法检测HBsAg呈阳性且S/CO≤10的标本为研究对象,对所有标本采用HBsAg确认试剂盒以及配套使用的ELISA法进行检测,比较检测结果。结果53例科华ELISA检测HBsAg阳性患者,经确认试剂盒及其配套ELISA法检测,阳性的45例(84.9%),8例(15.1%)确认试验为阴性,并经科华试剂复测结果亦为阴性。结论对低浓度阳性标本进行HBsAg ELISA法复检,并用中和试验去除假阳性结果,能够有效避免报告错误率,减少手术,输血及器官移植等安全隐患。%ObjectiveTo investigate the enzyme-linked immunoassay (ELISA) in low concentration effect of HBsAg detection.Methods In January 2014~December 2014 at our hospital outpatient and hospitalized patients in 53 patients with progress ELISA method to detect HBsAg positive and S/CO specimens of 10 or less as the research object, for all specimens using HBsAg conifrm kit as wel as supporting the use of ELISA method for testing, comparing test results.Results Kehua ELISA detected 53 cases of HBsAg positive patients, conifrmed kit and supporting by ELISA. Positive results of 45 cases (84.9%), 8 cases (15.1%) of the validation test were negative, and the progress reagent of retest results are negative. Conclusion HBsAg ELISA method for low concentration samples recheck, and neutralization test to remove false positive results, can effectively avoid the report error rate, reduce the surgery, blood transfusion and organ transplantation and other security hidden danger.

  3. Advanced characterization of MIMAS MOX fuel microstructure to quantify the HBS formation

    Energy Technology Data Exchange (ETDEWEB)

    Bouloré, Antoine, E-mail: antoine.boulore@cea.fr [CEA, DEN, DEC Fuel Research Department, Cadarache, F13108 Saint-Paul-lez-Durance (France); Aufore, Laurence; Federici, Eric [CEA, DEN, DEC Fuel Research Department, Cadarache, F13108 Saint-Paul-lez-Durance (France); Blanpain, Patrick [AREVA NP SAS, 10 rue Juliette Récamier, F-69456 Lyon (France); Blachier, Rémi [EDF, SEPTEN, 12-14 Av. Dutrievoz, F-69628 Villeurbanne (France)

    2015-01-15

    Highlights: • An advanced characterization of MIMAS MOX fuel based only on fresh fuel pellet characterization. • A probabilistic approach to model the High Burnup Structure formation in oxide fuels. • Validation of the method by comparing to experimental data obtained on fuel irradiated in the Halden reactor. - Abstract: Fission gas behaviour in accidental situations is closely related to the location of fission gas before the accident. More precisely, most of the fission gas in intergranular position is released during the accident and HBS zones contribute a lot to this intergranular quantity. So a methodology to characterize the HBS zones a priori from examination of unirradiated pellet has been developed at CEA. Characterization of plutonium distribution in MIMAS MOX fresh fuel pellets can be performed by image analysis on 1 mm{sup 2} X-ray mappings of plutonium acquired using Electron Probe Micro Analysis (EPMA). The specific software developed to describe the fuel using Pu X-ray mapping (ANACONDA) has been improved in order to simulate the fission products (FP) production and recoil during a given irradiation of the fuel, taking into account the evolution of the plutonium due to neutron irradiation. This simulation results from calculations with our fuel performance code ALCYONE combined with image processing. The final result is a mapping of local burn-up, but also the distribution of the relative FP concentration as a function of the local burn-up. A validation of this simulation process has been done by comparing the simulated mapping of neodymium to one measured on the same fuel batch after irradiation. Using previous studies of mechanisms for HBS formation, a probabilistic criterion for HBS formation has been proposed, based on the EPMA measurements of the decrease of the xenon signal as a function of the local burn-up. Combining the simulated FP cartography with this probabilistic HBS formation criterion, it is possible to calculate the surface

  4. Serum anti-hepatitis B surface antigen in hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Rafieian-Kopaei Mahmoud

    2012-01-01

    Full Text Available To evaluate the immune response to hepatitis B vaccination in stable hemodialysis (HD patients, a retro-prospective investigation was conducted on 68 HD patients. Participants were vaccinated against hepatitis B virus with an intramuscular hepatitis B vaccination schedule, 40 micrograms at 0, 1, and 6 months. The serum antibody level against hepatitis B surface antigen (HBs in HD patients was 35±55. In this study, no significant differences of Anti-HBs antibody between diabetic and non-diabetics or male and female subjects were observed. There were not any significant correlation between antibody against HBs-Ag and serum albumin. There was not significant correlation between anti-HBs antibody and age, proportion of HD, duration of HD or dialysis efficacy. In this study, there was not significant correlation between serum antibody level against hepatitis B surface antigen and some demographic indices of HD patients, however, these findings need to re-test in other centers with more participants.

  5. Clinical significance of isolated anti-HBc positivity in cases of chronic liver disease in New Delhi, India

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    Manisha Jain

    2009-01-01

    Full Text Available Background: The presence of anti-HBc IgG in the absence of HBsAg is usually indicative of a past self-limiting HBV infection. But it is frequently associated with co-infection with HCV which can worsen the existing status of chronic liver disease (CLD. Objectives: The present study was planned to evaluate the significance of isolated HBc IgG positivity in patients of CLD and look for the presence of HCV co-infection in such patients. Methods: Clinical profiles and biochemical tests were done for all the 77 CLD cases included in the study. Blood samples were taken from these patients and tested by the commercially available EIA for the presence of HBsAg, anti-HBc IgG, anti-HBs and anti-HCV. HBV DNA was detected by amplifying the surface region in all the cases. Results: Isolated anti-HBc IgG positivity defined as the presence of anti-HBc IgG in absence of any other serological markers of HBV infection was detected in 28 patients . Out of 64 patients positive for anti-HBc IgG 36 had the markers of HBV, either HBsAg, HBV DNA or anti-HBs alone or in combination. There was a significant association between isolated anti-HBc IgG positivity and HCV co-infection. Conclusion: Anti-HBc IgG should be tested in all patients with CLD as it is frequently the only marker of HBV infection in such patients and they should be monitored closely as such patients can develop CLD. Presence of co-infection with HCV should be actively searched for in such patients.

  6. Soroprevalência de anticorpos contra vírus herpes simples 1-2 no Brasil Seroprevalencia de anticuerpos contra virus herpes simples 1-2 en Brasil Seroprevalence of herpes simplex 1-2 antibodies in Brazil

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    Sue Ann Costa Clemens

    2010-08-01

    de sangre para detección de anticuerpos para HSV-1 y HSV-2 con prueba tipo-específica ELISA gG1-gG2. Fueron descritas frecuencias y proporciones y comparadas entre grupos utilizando la prueba de Fisher bilateral exacta. Fue realizado análisis de regresión logística para evaluar influencia de las variables edad, sexo, geografía, grupo económico, histórico de DST, seropositividad para anti-HSV-1 o anti-HSV-2 e interacciones de cualquiera de esos factores sobre la seroprevalencia de HSV-1 y/o HSV-2. RESULTADOS: La tasa de seroprevalencia de anticuerpos para HSV-1 ajustada por edad fue de 67,2%, sin diferencia con relación al sexo, siendo mayor en la Región Norte. Las seroprevalencias aumentaron con la edad, y para HSV-2, hube un aumento significativo en la adolescencia y entre adultos jóvenes. Individuos seropositivos para HSV-1 presentaron mayor riesgo de ser positivos para HSV-2 (15,7% cuando se compararon con los negativos para HSV-1 (4,7%. En el análisis multivariado, el histórico de DST aumentó significativamente (OR=3,2 la probabilidad de seropositividad para HSV-2. CONCLUSIONES: Las seroprevalencias para HSV-1 y para HSV-2 varían con la edad y presentan diferencias significativas entre las regiones de Brasil. Historia anterior de DST es importante factor de riesgo para adquisición de infección por HSV-2.OBJECTIVE: To estimate the seroprevalence of HSV-1 and HSV-2 antibodies in Brazil and to analyze factors associated. METHODS: Cross-sectional study including subjects aged 1-40 years from the general population in four different geographical areas in Brazil between 1996 and 1997. All subjects were stratified by age and gender and 1,090 of them were included in the final analysis. Blood samples were tested for HSV-1 and HSV-2 antibodies by type-specific (gG1 and gG2 ELISA. Frequencies and proportions were described and compared among groups using two-sided Fisher's exact test. A logistic regression analysis was performed to assess the influence of

  7. Intra-familial prevalence of hepatitis B virologic markers in HBsAg positive family members in Nahavand, Iran

    Institute of Scientific and Technical Information of China (English)

    Amir Houshang Mohammad Alizadeh; Mitra Ranjbar; Shahin Ansari; Seyed Moayed Alavian; Hamid Mohaghegh Shalmani; Leila Hekmat; Mohammad Reza Zali

    2005-01-01

    AIM: To determine the prevalence of hepatitis B in Nahavand and evaluate the HBsAg positive prevalence in families with a member who was confirmed to have HBV infection.METHODS: This study was performed in two phases. In the first phase, 1 824 subjects in Nahavand city were selected. The interviewers visited the houses of chosen families to fill the questionnaire and take the blood samples.All subjects signed an informed consent before interviews and blood sampling. The samples were evaluated for HBV virologic markers. In the second phase, 115 HBsAg-positive cases were enrolled and evaluated for HBV virologic markers.RESULTS: The prevalence of positive HBsAg in Nahavand was 2.3%. The most frequent relatives of index cases were sons and daughters (32.2% and 23.5% respectively).Twelve (11%) of all family members were HBsAg positive.Fifty (56.2%) were isolated HBsAb positive and only one person (2.5%) was isolated HBcAb positive. The higher rates of HBsAg marker were detected in the brothers (1-25%) and fathers (1-12.5%). The infection rate in husbands and wives of index cases was 10%. Only two (16.7%) of all HBsAg-positive participants reported previous HBV vaccination.CONCLUSION: The prevalence of intra-familial HBV infection is lower in Nahavand of Iran compared to other studies.More attention should be paid to HBV vaccination and risk-lowering activities.

  8. Presencia de anticuerpos sericos contra Neospora caninum en equinos en Chile Sera antibodies to Neospora caninum in Chilean horses

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    A.N. Patitucci

    2004-12-01

    Full Text Available Neospora sp. es un parásito protozoario causante de abortos y/o enfermedad del Sistema Nervioso Central (SNC en caninos, bovinos, ciervos, caprinos, ovinos, equinos y otros. En equinos, se lo ha involucrado como causa de aborto, mortalidad neonatal y enfermedades del SNC y viscerales. La especie que infecta a los equinos es distinta a Neospora caninum y se denomina Neospora hughesi. En Chile no existe evidencia de infección en equinos, sin embargo, la enfermedad ha sido informada en bovinos y caninos, por lo que el objetivo de este trabajo fue buscar anticuerpos contra la enfermedad en sueros equinos . Para ello se tomaron muestras de sangre a 145 equinos (87 de la IX Región y 58 de la VIII Región de Chile. Los sueros fueron analizados mediante Test de Aglutinación para Neospora (NAT. Cada suero se diluyó en 1:40 en solución salina tamponada (PBS pH 7.2 conteniendo 2- mercaptoetanol y fueron analizados en 1:40 y 1:80. Los sueros positivos a 1:80 fueron examinados a 1:160 y 1:320. Anticuerpos contra N.caninum (≥ 1:40 se encontraron en 32% (47/145 de los equinos estudiados. Debido a que N.caninum y N.hughesi dan reacción cruzada, ambos o uno de ellos podrían ser los responsables de la seropositividad. Estos resultados indican que equinos provenientes de la VIII y IX regiones de Chile son reaccionantes a la infección de Neospora, por lo que su diagnóstico presuntivo debería ser considerados ante la presencia de cuadros clínicos con sintomatología nerviosa y/o abortos en esta especie.Neospora caninum is a protozoan parasite that affects cattle and dogs, its infections seem to be widely prevalent in Chile. To date, nothing is known of the presence of N. caninum in horse population of the country. In South America, two seroprevalence studies have failed to find antibodies in Argentina and Brazil. The objective of the study was to investigate the presence of N. caninum exposure in horses of Chile. Sera from 145 asymptomatic horses (87

  9. A thirty-one year prospective follow-up program on the HBsAg carrier state and primary liver cancer in Qidong, China%乙型肝炎病毒感染与肝癌发生的31年随访研究

    Institute of Scientific and Technical Information of China (English)

    陈建国; 陆建华; 朱源荣; 朱健; 张永辉

    2010-01-01

    Objective To study the relationship between hepatitis B virus (HBV) and primary liver cancer (PLC), and to assess the outcome of PLC in the carriers of HBsAg. Methods General population over age of 15 from a community in Qidong was screened for HBsAg, anti-HBs,and alanine transaminase (ALT) in 1976, and followed-up thenceforth. From January 1, 1977 through December 31, 2007, 12 351 people were enrolled in the cohort, and their occurrence, outcome of PLC and other cancers, together with all the withdrawals due to death were linked to and checked with database from Qidong Cancer Registry and Qidong Vital Registry programs. Results The total observed person-years (PYs) were 355 305.0. One hundred and seventy-three PLC cases were identified among the HBsAg carriers, with an incidence of 361.55 per 100 000 PYs, while PLC cases were only 95 for the non-carriers, with an incidence of 30.90 per 100 000 PYs. The overall relative risk (RR)was 11.70(95%CI: 9.06-15.19) ,with RR 12.30 for men and 10.46 for women. HBsAg carriers had high incidence at each age group,compared with the non-carriers for both men and women. Data from cross-over analysis showed that the incidence rates of PLC for the sub-cohorts of female non-carriers,male non-carriers, female carriers, and male carriers were 1.00,3.07, 10.46, and 37.76, respectively.The cumulative rates of PLC in the 4 groups were 0.86%, 2.73%, 10.22%, and 34.19%, respectively.Results from aon-conditional logistic regression model showed that the gender (male), age, HBsAg ( + ), and ALT( + ) were risk factors for the development of PLC while anti-HBs( + ) demonstrated a protective effect. No relationship was found among carriers and non-carriers for cancer sites such as lung, stomach, esophagus, intestine, pancreas, breast, cervix, bladder, and lymphoma, brain tumor, or leukemia. Conclusion Causation and its strength, together with specificity and persistency of the association were confirmed from this HBsAg-related cohort study in

  10. DETECCIÓN DE ANTICUERPOS IgM A LEPTOSPIRA EN HUMANOS EN RIESGO OCUPACIONAL EN VILLAVICENCIO, META

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    Luz Díaz P

    2008-04-01

    Full Text Available Objetivo. Determinar la seroprevalencia a la infección por leptospira en una población humana y los factores de riesgo ocupacional asociados en Villavicencio, Meta. Materiales y métodos. Se aplicó un modelo epidemiológico transversal con selección por conveniencia de grupos e individuos dentro de grupos. Se obtuvieron muestras de sangre por punción cubital de 273 personas correspondientes a 8 grupos de riesgo. Se obtuvo información de factores de riesgo mediante entrevista. Se determinaron anticuerpos IgM mediante ELISA indirecta. Se utilizó una prueba de c2 mediante el programa SPSS 11.0 para Windows. Resultados. La seroprevalencia general fue 19%. Por grupos fue para trabajadores de matadero 7%, veterinarios y auxiliares de clínica de pequeños animales 17%, estudiantes de último año de MVZ 17%, ordeñadores 21%, trabajadores de arrozales 23%, trabajadores de granjas porcícolas 35%, trabajadores de piscícolas 48%. Se encontraron 3 factores asociados, el estrato rural p = 0.0005 ICCR 1.50 a 3.83, tenencia de mascota canina p = 0.046 ICCR 1.03 a 3.26 y contacto con roedores en el trabajo p = 0.000037 ICCR = 1.73 a 4.75. Conclusiones. Se encontró una alta seroprevalencia general de infectados por Leptospira, siendo los grupos de más alta prevalencia los trabajadores piscícolas y de granjas porcícolas. Se reconoce una vez más el carácter ocupacional de la infección posiblemente por falta de medidas de higiene y protección laboral.

  11. Seroprevalence of hepatitis B e antigen (HBe antigen and B core antibodies (IgG anti-HBcore and IgM anti-HBcore among hepatitis B surface antigen positive blood donors at a Tertiary Centre in Nigeria

    Directory of Open Access Journals (Sweden)

    Akinbami Akinsegun A

    2012-03-01

    Full Text Available Abstract Background Hepatitis B virus (HBV is a common cause of liver disease throughout the world. HBV is transmitted through blood and other body fluids, including semen and saliva. Chronic replication of HBV virons is characterized by persistence circulation of HBsAg, HBeAg and HBV DNA; usually with anti-HBc and occasionally with anti-HBs. Aim: To determine the prevalence of HBeAg, IgG anti-HBcore and IgM anti-HBcore amongst HBsAg positive blood donors. These parameters are reflective of transmissibility and active hepatitis B infection. A cross sectional study was carried out at the blood donor clinics of Lagos State University Teaching Hospital Ikeja and Lagos University Teaching Hospital Idiaraba. A total of 267 donors were recruited to determine HBe antigen, IgG and IgM anti-HBcore antibodies amongst hepatitis BsAg positive donors. Five milliliters of blood was collected from those who tested positive to HBsAg screen during donation. The sera were subjected to enzyme linked immunosorbent assay (ELISA. Pearson chi-squared test was used for the analytical assessment. Findings A total number of 267 HBsAg positive blood donors were studied. A seroprevalence of 8.2% (22 of 267 HBeAg was obtained, 4 of 267 (1.5% were indeterminate while 241 (90.3% tested negative. Only 27 out of 267 donors (10.1% tested positive to IgM anti-HBcore, 234(87.6% tested negative, while 6(2.2% were indeterminate. A higher percentage of 60.7% (162 of 267 tested positive to IgG anti-HBcore, while 39.3% (105 of 267 tested negative. Conclusion There is a low seroprevalence rate of HBeAg-positive chronic hepatitis and relatively high IgG anti-HBcore and IgM anti-HBcore rates in South West Nigeria.

  12. Expression of Hepatitis B virus surface antigen (HBsAg from genotypes A, D and F and influence of amino acid variations related or not to genotypes on HBsAg detection

    Directory of Open Access Journals (Sweden)

    Natalia M. Araujo

    2009-08-01

    Full Text Available The impact of hepatitis B virus (HBV genotypes on the sensitivity of surface antigen (HBsAg detection assays has been poorly investigated. Here, plasmids carrying consensus or variant coding sequences for HBV surface proteins from genotypes A, D and F, were constructed. HBsAg levels were evaluated in medium and extracts of transfected CHO cells by a commercial polyclonal-based assay. We show that HBsAg detection values of consensus forms from genotypes D and F were, respectively, 37% and 30% lower than those obtained by genotype A. However, the presence of two single variations, T143M in genotype A, and T125M in genotype D, produced a decrease of 44% and an increase of 34%, respectively, on HBsAg mean values in comparison with their consensus forms. In conclusion, HBsAg detection levels varied among HBV genotypes. However, unique amino acid substitutions not linked to genotypes, such as T125M and T143M described here, should have more implications in HBV immunological diagnostics than the set of variations characteristic of each HBV genotype.

  13. Asociación entre anticuerpos antifosfolípidos y complicaciones de la gestación en mujeres de Costa Rica

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    Roubier Rojas-Barahona

    2007-04-01

    Full Text Available Objetivo: Determinar asociación entre los anticuerpos antifosfolípidos y complicaciones de la gestación (aborto de cualquier tipo, preeclampsia, eclampsia, insuficiencia placentaria grave o parto antes de la semana 34 de gestación, tomando como base la población de pacientes del servicio de Obstetricia del Hospital México. Materiales y métodos: Estudio de casos y controles en la población de pacientes atendidas por el servicio de Obstetricia del Hospital México en el período comprendido entre enero de 2002 y diciembre de 2004. Se tomaron los datos del registro de egresos Hospitalarios, se hizo un análisis descriptivo, en análisis univariado y multivariado de las principales variables registradas. Resultados: El promedio de edad de las pacientes en los casos y los controles no mostró diferencia significativa. La provincia de San José reporta el mayor porcentaje de casos con un 39.9%, seguido por Heredia con un 26.8% y Alajuela con un 24.6%. En cuanto al número de gestaciones, se determinó que no influyen significativamente en relación con el aborto y otras complicaciones de la gestación. El análisis univariado y multivariado mantiene asociación entre los antecedentes clínicos de las gestaciones anteriores, además de los anticuerpos antifosfolípidos respecto al aborto y otras complicaciones de la gestación. Conclusión: Los anticuerpos antifosfolípidos deberían ser tomados en cuenta como parte del seguimiento del embarazo en pacientes con historia de abortos y complicaciones de la gestación en embarazos previos.

  14. OBTENCIÓN DE ANTICUERPOS ESPECÍFICOS PARA LA DETECCIÓN DEL VIRUS TaLMV DEL TOMATE DE ÁRBOL

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    Gutiérrrez Sánchez Pablo Andrés

    2011-08-01

    Full Text Available En Colombia el rendimiento del cultivo de árbol se ha visto seriamente afectado por la expansión de la enfermedad conocida como virosis. Esta patología se registró inicialmente en 1991 en el norte de Antioquia y su expansión ha alcanzado todas las regiones cultivadoras de este frutal en el país. Trabajos recientes han establecido la presencia de al menos dos especies de potyvirus asociadas a la virosis en los cultivos de tomate de árbol de Antioquia: Potato virus Y (PVY y Tamarillo leaf malformation virus (TaLMV, especie propuesta. Con el fin de reducir la diseminación de estos patógenos virales en el país, es necesario contar con herramientas de diagnóstico que permitan la certificación del material de siembra y la detección temprana en plantas asintomáticas. En este trabajo se presenta la obtención de anticuerpos específicos para la detección del virus TaLMV utilizando una región antigénica de 15 residuos de la cápside viral. La sensibilidad y especificidad de los anticuerpos fue evaluada mediante pruebas de ELISA y dot-blot utilizando proteína recombinante y péptidos sintéticos. La utilidad de estos anticuerpos fue validada a partir de una prueba piloto de detección de TaLMV en muestras de plantas de tomate de árbol obtenidas en el Oriente Antioqueño y los resultados comparados con los niveles de detección que ofrece la técnica de RT-PCR con cebadores específicos para la cápside viral de TaLMV.

  15. Does the antibody production ability affect the serum anti-Helicobacter pylori Ig G titer?

    Institute of Scientific and Technical Information of China (English)

    Hyun Ah Chung; Sun-Young Lee; Hee Won Moon; Jeong Hwan Kim; In-Kyung Sung; Hyung Seok Park; Chan Sup Shim; Hye Seung Han

    2016-01-01

    AIM: To investigate the relationship between serum titers of anti-Helicobacter pylori(H.pylori) immunoglobulin G(IgG) and hepatitis B virus surface antibody(HBsA b).METHODS: Korean adults were included whose samples had positive Giemsa staining on endoscopic biopsy and were studied in the hepatitis B virus surface antigen(HBsA g)/HBsA b serologic assay,pepsinogen(PG) assay,and H.pylori serologic test on the same day.Subjects were excluded if they were positive for HBs Ag,had a recent history of medication,or had other medical condition(s).We analyzed the effects of the following factors on serum titers of HBsA b and the anti-H.pylori IgG : Age,density of H.pylori infiltration in biopsy samples,serum concentrations of PG Ⅰ and PG Ⅱ,PG Ⅰ/Ⅱ ratio,and white blood cell count.RESULTS: Of 111 included subjects,74(66.7%) exhibited a positive HBsA b finding.The serum anti-H.pylori IgG titer did not correlate with the serum HBsA b titer(P = 0.185); however,it correlated with the degree of H.pylori infiltration on gastric biopsy(P < 0.001) and serum PG Ⅱ concentration(P = 0.042).According to the density of H.pylori infiltration on gastric biopsy,subjects could be subdivided into those with a marked(median: 3.95,range 0.82-4.00)(P = 0.458),moderate(median: 3.37,range 1.86-4.00),and mild H.pylori infiltrations(median: 2.39,range 0.36-4.00)(P < 0.001).Subjects with a marked H.pylori infiltration on gastric biopsy had the highest serological titer,whereas in subjects with moderate and mild H.pylori infiltrations titers were correspondingly lower(P < 0.001).After the successful eradication,significant decreases of the degree of H.pylori infiltration(P < 0.001),serum anti-H.pylori IgG titer(P < 0.001),and serum concentrations of PG I(P = 0.028) and PG Ⅱ(P = 0.028) were observed.CONCLUSION: The anti-H.pylori IgG assay can be used to estimate the burden of bacteria in immunocompetent hosts with H.pylori infection,regardless of the HBsA b titer after HBV vaccination.

  16. ELISA测定HBsAg室内质控问题的探讨

    Institute of Scientific and Technical Information of China (English)

    范秀芹; 林凌云; 岳志刚; 李爱华

    2002-01-01

    @@ 室内质控是采用一系列的方法连续评价检测过程的稳定性,并保证检测结果的可靠性.目前,ELISA测定HBsAg(还有其他一些测定)结果以定性方式报告,本文对可否采用Levay-Jennings质控图及Westgard多规则进行探讨[1],报告如下.

  17. Bajo índice de sospecha para deficiencia de anticuerpos en niños sometidos a procedimientos quirúrgicos de Otorrinolaringología

    OpenAIRE

    AB Ramírez-López; CG Zúñiga-Lagunes; A Martínez-Viveros; EA Medina-Torres; C Murata; SE Espinosa-Padilla; SO Lugo-Reyes

    2016-01-01

    INTRODUCCIÓN: las inmunodeficiencias primarias están subdiagnosticadas en todo el mundo, aun en centros de tercer nivel de atención. Los defectos de anticuerpos constituyen el grupo más prevalente y suelen manifestarse clínicamente después de los 6 meses de edad como infecciones respiratorias recurrentes causadas por bacterias encapsuladas. Algunas cirugías de oído-nariz y garganta se indican en pacientes con infecciones respiratorias recurrentes o complicadas que no han respondido adecuadame...

  18. Anticuerpos monoclonales específicos de inmunoglobulina G canina: caracterización y aplicación en inmunoensayos

    OpenAIRE

    2011-01-01

    En este trabajo se ha conseguido aislar la IgG+IgM de perro a partir de sueros de animales sanos y enfermos, considerado de gran interés dada la escasez de este tipo de reactivos en el mercado y de la importancia de los estudios inmunologicos en las especies de interes veterinario. A partir de la IgG comercial se ha producido y posteriormente caracterizado un panel de seis anticuerpos (CA2E9, CA3B8, CA3H1, CA4E7, CA4F1, CA5B2) frente a dicha inmunogloblulina. Dada la reaccion cruzada de los a...

  19. Encefalitis límbica autoinmune por anticuerpos anticanales de potasio dependientes de voltaje: a propósito de un caso

    OpenAIRE

    2016-01-01

    La encefalitis límbica (EL) se ha descrito como una entidad de origen viral o autoinmunitario secundaria a la presencia de autoanticuerpos antineuronales. Una de estas formas de presentación se asocia a la presencia de anticuerpo frente a canales de potasio voltaje dependientes (CKVD). Clínicamente se manifiesta como pérdida de memoria subaguda, cambios de comportamiento, desorientación progresiva, crisis epilépticas, trastornos psiquiátricos, insomnio, depresión, alucinaciones e hiponatremia...

  20. Complicaciones clínicas en una serie de pacientes con anticuerpos anticardiolipina Clinical complications in a series of patients with anticardiolipin antibodies

    OpenAIRE

    Pujol, A; E. Alegría; J González; Ch. Ngare; J. Pérez; J. A. Páramo

    2003-01-01

    Se han analizado las complicaciones clínicas determinadas con métodos objetivos en 68 pacientes de la Clínica Universitaria de Navarra con sospecha clínica de síndrome antifospolípido (SAF) y presencia de anticuerpos anticardiolipina (ACA). Se consideraron para el estudio, pacientes con título de IgG superior a 23 GPL. La patología más prevalente fue la trombosis: trombosis venosas (42,6%) y arteriales (22%). Otras complicaciones fueron abortos (23,8% de las 42 mujeres) y trombocitopenia (12,...

  1. Prueba de Elisa indirecta para la detección de anticuerpos IgM para el diagnóstico de Leptospirosis humana

    OpenAIRE

    Manuel Céspedes Z; Martha Glenny A; Vidal Felices A; Lourdes Balda J; Víctor Suárez M

    2002-01-01

    Para el diagnóstico temprano de enfermedades con cuadro clínico inespecífico como la leptospirosis, es necesario la confirmación laboratorial mediante pruebas específicas, con la finalidad de que el diagnóstico sea más acertado y rápido. Objetivo: se realizó un estudio comparativo entre la prueba de microaglutinacion (MAT) y la prueba de ELISA indirecta estandarizada con un "pool" de antígenos de Leptospira interrogans, para la detección de anticuerpos IgM, en muestras de suero de fase aguda ...

  2. Anticuerpos antinucleares, imágenes y características obtenidas por inmunofluorescencia: Importancia de los isotipos IgA, IgM e IgG Antinuclear antibodies, patterns and characteristics obtained by immunofluorescence: The importance of the IgA, IgM and IgG isotypes

    Directory of Open Access Journals (Sweden)

    Miriam Arcavi

    2009-10-01

    Full Text Available La técnica de elección para el screening de anticuerpos antinucleares (ANA es la inmunofluorescencia indirecta que utiliza como sustrato una línea de células epiteliales de carcinoma de laringe humano (IFI-HEp2, y como antisuero, anti-IgG o anti-Ig totales. Los ANA-IgG son los más importantes para el diagnóstico y monitoreo de las enfermedades del tejido conectivo (ETC, mientras los ANA-IgM son de menor relevancia clínica en estos pacientes. Sin embargo, poco se sabe de los ANA-IgA ya que estos Ac han sido menos investigados. El objetivo de este trabajo fue estudiar la prevalencia de los diferentes isotipos de inmunoglobulinas de anticuerpos antinucleares en los pacientes con ETC y evaluar la conveniencia de utilizar conjugados monovalentes o polivalentes. Se procesaron 100 sueros de pacientes con diversas ETC empleando IFI-HEp2, en los cuales se detectó 38% de ANA-IgA (títulos ≥ 1:80 y 12% de ANA-IgM (títulos ≤ 1:160. En 29 casos se detectó IgA en ausencia de IgM, en 3 casos IgM en ausencia de IgA. En todos los casos los ANA-IgG estuvieron presentes. En 6 sueros se observó un cambio de imagen con conjugado anti-IgA y en 3 con conjugado anti-IgM. Debido a la alta prevalencia de ANA-IgA detectada por IFI-HEp2, se destaca la conveniencia de utilizar conjugado anti-Ig totales en lugar de anti-IgG, mientras se desconozca la relevancia de los ANA-IgA en el diagnóstico, pronóstico y seguimiento de las enfermedades reumáticas sistémicas.The indirect immunofluorescence with epitelial cell line from human laryngeal carcinoma as substrate (IIF-HEp2 and anti-IgG or anti-total Ig as antisera, is the technique currently used for the detection of antinuclear antibodies. The most important antibodies for the diagnosis and follow-up of connective tissue diseases (CTD are the IgG-ANA, while the IgM-ANA have no clinical relevance. However the IgA-ANA have not been thoroughly investigated so far. The aim of this work was to study the prevalence

  3. 左旋咪唑搽剂治疗儿重HBsAg携带者效果研究%Therapeutic Effects of 60 cases of HBsAg carriers children treated with levamisole liniment

    Institute of Scientific and Technical Information of China (English)

    王德英; 刘相申; 王来祥

    2004-01-01

    目的观察左旋咪唑擦剂治疗儿童HBsAg携带者的疗效.方法应用左旋咪唑搽剂治疗儿童HBsAg携带者,并设立对照组60例.结果治疗3个月后,治疗组与对照组相比,HBV-DNA阴转率(35%比11.7%)差异有显著性.治疗6个月后,治疗组与对照组相比,HBV-DNA、HBeAg阴转率明显优于患者母亲HbeAg阳性组.结论左旋咪唑搽剂用于治疗儿童HBsAg携带者有效,安全可靠.

  4. Optimización del ensayo vibriocida colorimétrico para la determinación de anticuerpos séricos contra V. cholerae del serogrupo O139

    OpenAIRE

    Joivier Vichi-Lozada; Edith Suzarte-Portal; Talena Ledón-Pérez; Rafael Fando-Calzada

    2011-01-01

    El título de anticuerpos vibriocidas es reconocido actualmente como el mejor marcador de inmunidad protectora ante la infección con Vibrio cholerae. Su evaluación permite estudiar la efectividad de vacunas durante ensayos preclínicos y clínicos, a través de la determinación de la funcionalidad de anticuerpos séricos, capaces de lisar células en presencia de complemento exógeno. Se han desarrollado distintos ensayos bactericidas con puntos finales y métodos de determinación del título vibrioci...

  5. 医用臭氧自体血回输对慢性乙型肝炎患者 HBeAg、HBsAg 及 FibroScan 的影响%Effect of medical ozone autologous blood transfusion on FibroScan, HBsAg and HBeAg in patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    康海燕; 董江龙; 李健; 陈蕾; 叶立红; 王艳; 王晓静; 王晨雪

    2015-01-01

    目的:观察医用臭氧对慢性乙型肝炎患者HBeAg、HBsAg及瞬时肝弹性测定(FibroScan)的影响,评估医用臭氧在慢性乙型肝炎患者中的抗病毒、抗纤维化作用。方法将120例HBeAg阳性的慢性乙肝患者随机分成2组,治疗组给予医用臭氧自体血回输治疗(采集患者静脉抗凝全血100 mL,在其中缓慢注入100 mL 30μg/mL医用臭氧,然后再回输到静脉),每周3次;对照组给予聚乙二醇干扰素α-2 a 180μg皮下注射,每周1次。观察2组治疗4周、8周、12周时血清HBsAg、HBeAg定量及FibroScan的变化。结果2组在治疗4周、8周、12周时血清HBsAg、HBeAg定量及FibroScan均逐渐下降(P均<0.05);2组间各指标比较差异无统计学意义(P均>0.05);患者血清HB-sAg定量、HBeAg定量与FibroScan之间呈正相关(r=0.764,0.779,P均<0.05)。结论医用臭氧自体血回输治疗慢性乙型肝炎与聚乙二醇干扰素α-2a效果相当,其抗病毒、抗纤维化效果确切。%Objective It is to observe the influence of medical ozone on HBeAg , HBsAg and transient liver elasticity ( Fi-broScan ) in patients with chronic hepatitis B , and evaluate the antiviral and anti fibrosis effects of medical ozone in the treat -ment of chronic hepatitis B patients .Methods 120 patients with HBeAg positive chronic hepatitis B were randomly divided in-to two groups.The patients in the treatment group was treated with medical ozone autologous blood transfusion (anticoagulant venous blood 100 mL was collected from patients , which was slowly injected into the 100 mL 30μg/mL of medical ozone , and then returned to the vein ) , three times a week;the patients in the control group were treated with the polyethylene glycol inter -feron α-2a 180 μg by subcutaneous injection , once a week.The changes of serum HBeAg , HBsAg quantification and Fi-broScan value in the treatment of 4 weeks, 8 weeks, and 12 weeks were

  6. Prevalence of Serologic Hepatitis B Markers in Blood Donors From Puebla, Mexico: The Association of Relatively High Levels of Anti-Core Antibodies With the Detection of Surface Antigen and Genomic DNA

    Directory of Open Access Journals (Sweden)

    Sosa-Jurado

    2016-06-01

    Full Text Available Background The hepatitis B virus (HBV causes chronic hepatitis, hepatic cirrhosis, and hepatocellular carcinoma. Surface antigen (HBsAg detection is a definitive test that can confirm HBV infection, while the presence of antibodies against the core protein (anti-HBc suggests either a previous or ongoing infection or occult hepatitis B infection (OBI. Objectives The aim of the present study was to determine the prevalence of anti-HBc and HBsAg in blood donors. Further, the study aimed to estimate the anti-HBc level at which HBV DNA is detected in putative OBI cases, as well as to search for mutations in the “a” determinant associated with the non-detection of HBsAg in serum. Patients and Methods We conducted a cross-sectional study from 2003–2009. The study included 120,552 blood donors from the state of Puebla, Mexico. Different commercial systems based on microparticles (enzymatic (MEIA or chemiluminescent (CMIA were used to determine the HBsAg and anti-HBc levels. For the detection of HBV DNA, a nested polymerase chain reaction (nested PCR was used and the genotypes were determined using Sanger sequencing. Results Of the 120,552 blood donors, 1437 (1.19%, 95% CI: 1.12 - 1.26 were reactive to anti-HBc, while 82 (0.066%, 95% CI: 0.053 - 0.079 were reactive to HBsAg. Some 156 plasma samples collected in 2009 from anti-HBc-positive/HBsAg-negative blood donors were submitted for HBV DNA detection in a search for probable OBI. Viral DNA was detected in 27/156 (17.3%, 95% CI: 11.5 - 23.1. Our results show an association between HBV DNA or HBsAg and anti-HBc S/CO levels ≥ 4.0. All DNA samples were identified as genotype H and some “a” determinant mutations were identified, although none corresponded to mutations previously reported to hinder the detection of HBsAg by commercial immunoassays. Conclusions We observed that as the anti-HBc levels increase, there is a higher prevalence of the viral protein HBsAg in blood donors. Samples testing

  7. Validación del ensayo Vibriocida colorimétrico para determinar anticuerpos séricos contra cepas candidatas vacunales de Vibrio cholerae

    Directory of Open Access Journals (Sweden)

    Bárbara Cedré

    2003-03-01

    Full Text Available El Instituto Finlay posee un grupo de cepas atenuadas candidatas a vacuna oral contra el cólera, las cuales fueron evaluadas desde el punto de vista de su capacidad inmunogénica mediante la determinación de anticuerpos por el método ELISA y principalmente por el ensayo Vibriocida. Este ensayo mide la funcionalidad de los anticuerpos estimulados por la cepa vacunal, por lo que se considera la herramienta más eficaz para medir el estado de la inmunidad en individuos vacunados contra el cólera y constituye el soporte analítico para el desarrollo de la vacuna anticolérica cubana. En este trabajo se evaluaron la precisión, la especificidad y la robustez del ensayo y los resultados obtenidos estuvieron dentro del criterio de aceptación de esta técnica (>50% de coincidencia. Se demostró que las variaciones entre las muestras negativas y positivas se debían precisamente al crecimiento de V. cholerae y que se pueden incorporar pequeños cambios al ensayo sin que esto traiga consigo variaciones en el título de la muestra ni falsos resultados. De acuerdo con los resultados obtenidos, el mismo se considera apto para la evaluación de la respuesta inmune estimulada por un candidato vacunal contra el cólera.

  8. Determinación de IgG y anticuerpos totales contra el Virus Dengue, en muestras obtenidas en papel filtro

    Directory of Open Access Journals (Sweden)

    María García

    1997-01-01

    Full Text Available Se propone como alternativa para la determinación de anticuerpos contra el virus del dengue, el uso de muestras de sangre total obtenidos en papel filtro, por ser un método sencillo y no requerir de muchos cuidados en el envío al laboratorio. Se evaluaron 100 muestras de suero obtenidas en tubos al vacío y sangre total en papel filtro respectivamente, de pacientes con diagnóstico clínico de Dengue clásico. Ambas muestras fueron evaluadas con los métodos serológicos de ELISA Indirecta para detectar IgG e Inhibición de la Hemoaglutinación para detectar anticuerpos totales. Por ELISA indirecta se obtuvo un total de 51 positivos y 49 negativos en suero; 45 positivos y 48 negativos en papel filtro, con una concordancia por índice Kappa de 0.92, sensibilidad de 88% y especificidad de 98%. Por Inhibición de la Hemaglutinación se obtuvo un total de 45 positivos y 55 negativos en suero; 40 positivos y 50 negativos en papel filtro, con una concordancia por índice Kappa de 0,88, sensibilidad de 87% y especificidad de 91%.

  9. Detección de anticuerpos contra Borrelia burgdorferi e identificación de garrapatas ixodidas en Piura Y Amazonas, Perú

    Directory of Open Access Journals (Sweden)

    Martha Glenny A

    2004-01-01

    Full Text Available Objetivos: Detectar anticuerpos IgG/IgM contra Borrelia burgdorferi en población general, procedentes de los departamentos de Piura y Amazonas e identificar especies de garrapatas probablemente incriminadas en la transmisión de la enfermedad de Lyme. Material y Métodos: Entre agosto del año 2001 y junio de 2002, se colectaron muestras de sangre de 232 pobladores procedentes de ocho localidades del Departamento de Piura y 12 del Departamento de Amazonas, para evaluar mediante ELISA Captia™ Lyme IgG/IgM (Trinity biotech la presencia de anticuerpos contra Borrelia burgdorferi. Además, se colectaron garrapatas en animales domésticos por búsqueda directa. Resultados: Se detectó seropositividad en 9,9 % de los sueros evaluados. Asimismo, de 433 garrapatas colectadas se identificaron los géneros: Ixodes (5,5%, Amblyomma (18,0%, Rhipicephalus (23,5%, Anocentor (31,1% y Boophilus (21,7%. Conclusiones: Existen personas seropositivas por Borrelia en Piura y Amazonas, coincidiendo con los hallazgos realizados en Sapillica en el año 1992, además se detectó la presencia de garrapatas del género Ixodes en Piura.

  10. 制备源自HBsAg基因修饰树突状细胞的外切体%Generation of exosomes derived from adenovirus-mediated HBsAg gene-modified dendritic cells

    Institute of Scientific and Technical Information of China (English)

    杨静悦; 高琳; 付蓉; 薛妍; 刘文超

    2012-01-01

    Objective: To obtain exosomes derived from adenovirus - mediated HBsAg gene - modified dendritic cells. Methods: Full length HBsAg cDNAs were cloned into shuttle2 vector. The HBsAg gene fragments resulted from the - S digested with PI - See and I - Ceu were linked to the linear adeno - X virus DNA. After packaged with HEK293 cells, the adenovirus expression vector was obtained. Then the recombinant adenovirus expression plasmid AdVHBsAg was transfected into human monocyte - derived dendritic cells. The exosomes were isolated from superna-tant of transfected DCs. Transmission electron microscopy was used to observe their structures. The expressions of several proteins were investigated by flow cytometry. Results: The shuttle2 - S showed that band with 630 bp by di-gested with PI - See and I - Ceu, HBsAg gene in the inserted DNA of AdVHBsAg was confirmed by PCR, and pre-dictive fragments proved by restriction enzyme digestion analysis were exhibited. CPE appear 10 after days HEK293 cells transfected AdVHBsAg. Application of the isolation procedure to transfected DCs revealed exosome vesicles by transmission electron microscopy. Protein analysis by Western blot was performed and revealed that the costimulatory molecule CD86,CD83 and HBsAg was detectable. Conclusion; The exosomes derived from HBsAg - DC may be a tool of the HBV related hepatocellular carcinoma immunotherapy.%目的:制备一种新型负载HBsAg基因的外切体(exosome)瘤苗,并探讨其生物学特性、免疫学功能.方法:运用分子克隆和病毒载体转染HBsAg基因构建AdVHBsAg-DC肝癌瘤苗,采用流式细胞术鉴定转染基因表达;提取exosome;以透射电镜观察、Western blot法鉴定exosome.结果:构建的重组AdVHBsAg腺病毒载体,经PCR和酶切鉴定,结果显示HBsAg基因片段已正确插入腺病毒载体中.包装的腺病毒载体具有良好的感染性,可以在293细胞中形成病毒颗粒.提取的exosome在透射电镜下可观察到直径为50-100nm

  11. Eficacia experimental de anticuerpos IgY producidos en huevos, contra el veneno de la serpiente peruana Bothrops atrox Experimental efficacy of IgY antibodies produced in eggs against the venom of the Peruvian snake Bothrops atrox

    Directory of Open Access Journals (Sweden)

    Julio C. Mendoza

    2012-03-01

    Full Text Available Objetivos. Desarrollar un protocolo de inmunización para producir inmunoglobulinas IgY de origen aviar contra el veneno de la serpiente peruana Bothrops atrox y evaluar la capacidad neutralizante. Materiales y métodos. Se inmunizaron seis gallinas de postura de la raza hy line brown con 500 μg/dosis de veneno de B. atrox en un periodo de dos meses. Cada semana, los huevos fueron colectados para el aislamiento de inmunoglobulinas IgY a partir de la yema, usando dos pasos consecutivos con αcido caprνlico y sulfato de amonio. La detecciσn de anticuerpos se realizσ por inmunodifusiσn doble mientras que el tνtulo y reactividad cruzada se determinaron por las técnicas de ELISA y Western blot. El cálculo de DL50 y de la DE50 del antiveneno IgY producido se realizó utilizando el método de Probits. Resultados. La masa de anticuerpos aislados fue de 8,5 ± 1,35 mg de IgY/mL de yema. Asimismo, la DE50 del antiveneno aviar fue calculada en 575 μL de antiveneno/mg de veneno. Adicionalmente, los ensayos de reactividad cruzada mostraron que el veneno de B. atrox comparte mas epνtopes comunes con el veneno de B. brazili (47% que con otros veneno del mismo género, en tanto que los venenos de Lachesis muta (19% y Crotalus durissus (12% mostraron una baja reactividad cruzada. Conclusiones. Se ha obtenido IgY purificada contra el veneno de B. atrox con capacidad neutralizante y se ha demostrado su utilidad como herramienta inmunoanalítica para evaluar la reactividad cruzada con venenos de otras especies.Objectives. To develop an immunization protocol in order to produce avian IgY immunoglobulins against Bothrops atrox Peruvian snake venom and to evaluate its neutralizing capacity. Materials and methods. Six Hy Line Brown hens were immunized each two weeks using 500μg/doses of B. atrox venom in a period of two months. Each week, eggs were collected for IgY isolation from yolk using two consecutive steps with caprilic acid and ammonium sulfate

  12. Estudio del perfil de anticuerpos contra Helicobacter pylori producidos por pacientes con diferentes patologías gastroduodenales

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    Spinel Clara

    2004-12-01

    Full Text Available La colonización de la mucosa gástrica con Helicobacter pylori causa la infección bacteriana más frecuente a nivel mundial que es la mayor causa de enfermedades gastrointestinales en humanos. Con el objetivo de examinar el reconocimiento de antígenos de dos cepas de la bacteria en
    150 pacientes infectados y con inflamación (30 de gastritis crónica no atrófica G, úlcera duodenal (30 UD, patologías preneoplásicas (30 de gastritis atrófica GA y 30 de metaplasia intestinal MI y adenocarcinoma gástrico (30 C, se realizó un sistema de cultivo de Helicobacter pylori
    con células epiteliales gástricas. Los sobrenadantes de estos cocultivos fueron empleados como preparación antigénica en pruebas de inmunotransferencia, en las que se detectaron los anticuerpos
    IgG totales, al igual que sus cuatro subclases (IgG1, IgG2, IgG23, IgG4 en los sueros de los pacientes. Se encontró que los perfiles antigénicos de las dos cepas de Helicobacter pylori, aisladas de un paciente con úlcera duodenal y uno con adenocarcinoma gástrico, eran semejantes, con 23 proteínas comunes que incluían los principales factores de virulencia de la bacteria (CagA, VacA, Ureasa, Flagelina. Aunque los sueros mostraron un reconocimiento variado de cada uno de los antígenos, no se encontraron diferencias entre el número de antígenos reconocidos según la patología ni la cepa, tanto para las IgG totales (p = 0,98, como cada una de las subclases (IgG1:p = 0,97; IgG2: p = 0,72; IgG3: p = 0,61; IgG4: p = 0,84. Con el fin de analizar simultáneamente la respuesta de los pacientes hacia el total de los 23 antígenos, se aplicaron análisis multivariados gracias a los cuales se distinguieron cuatro proteínas, de 70,
    82, 90 kDa y VacA de 86 kDa como los principales antígenos implicados en la respuesta inmune de las personas infectadas. Adicionalmente, se diferenciaron dos grupos de patologías según el reconocimiento del conjunto de antígenos: por

  13. Hepatitis B virus infection status in the PBMC of newborns of HBsAg positivemothers

    Institute of Scientific and Technical Information of China (English)

    Su Ping Wang; De Zhong Xu; Yong Ping Yah; Meng Yuan Shi; Ru Lin Li; Jing Xia Zhang; Gang Zuan Bai; Jian Xin Ma

    2000-01-01

    AIM To study the hepatitis B virus (HBV) status in peripheral blood mononuclear cells (PBMC) and itsrelationship with serum HBV infection in newborns of hepatitis B surface antigen (HBsAg) positive mothers.METHODS Blood specimens were collected by femoral puncture from newborns of HBsAg positive motherswithin 24 hours after birth between February, 1997 and May, 1998. All sera were examined for HBV DNAand HBsAg by nested polymerase chain reaction (nPCR) and enzyme-linked immunosorbent assay (ELISA).PBMC were separated from above blood specimen of newborns. Fifty-five FBMC smear of newborns wereobtained whose sera were HBV DNA positive and 38 PBMC smear were randomly selected from newbornswhose'sera were HBV DNA negative. These Ninety-three smear of newborns' PBMC at birth were detectedfor HBV DNA by in situ polymerase chain reaction (IS PCR) and in situ hybridization (ISH) using digoxin-labelled HBV DNA probe.RESULTS Twenty-seven (49.09%) out of 55 HBV DNA positive newborns sera had HBV DNA in PBMCand 4 (10.53%) out of 38 HBV DNA negative newborns sera were detected for HBV DNA in their PBMC byISH. Sixty-two HBV DNA negative newborns PBMC by ISH were examined for HBV DNA by IS PCR. Ten(35.71%) out of 28 HBV DNA positive newborns sera had HBV DNA in their PBMC. Two (5.88%) out of 34 HBV DNA negative newborns sera were found HBV DNA in their PBMC. Total positive rates of PBMCHBV DNA (by ISH and/or IS PCR) were 67.27% (37/55) in those newborns with HBV DNA positive seraand 15.79% (6/38) in those newborns with HBV DNA negative sera.CONCLUSION HBV DNA in PBMC were found in most of newborns who had HBV DNA positive sera.But HBV DNA in PBMC also were positive in some of newborns who were negative for HBV DNA in theirsera at birth. It suggests that intrauterine HBV infection may be demonstrated only by HBV infection intheir PBMC and should be served as diagnosis index for intrauterine HBV infection. HBV infection in PBMCmay play some role in HBV intrauterine infection and

  14. Prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre del IMSS, Orizaba, Veracruz, México Prevalence of antibodies against Trypanossoma cruzi in blood bank donors from the IMSS General Hospital in Onizaba, Veracruz, Mexico

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    Angel Ramos-Ligonio

    2006-02-01

    Full Text Available OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70 y un extracto crudo de epimastigotes. Las muestras fueron obtenidas entre los meses de octubre de 2001 a enero de 2002. RESULTADOS: Los 420 donadores de sangre analizados fueron seronegativos para HBV, HCV, BrA, VDRL y HIV. Después del tamizaje de los 420 donadores, se identificaron dos individuos seropositivos por las pruebas de ELISA, Western blot e IFI, con una seroprevalencia de 0.48%. CONCLUSIONES: En este estudio se muestran evidencias de seropositividad para T. cruzi en donadores de sangre del HGRO, lo que sugiere la existencia de riesgo de contaminación por transfusión sanguínea. Por tal motivo, es necesario aplicar programas para el tamizaje serológico a través de técnicas inmunológicas con alta sensibilidad y especificidad.OBJECTIVE: To estimate the prevalence of antibodies against Trypanosoma cruzi in blood donors from Hospital General Regional (HGRO of the Mexican Institute of Social Security (IMSS per its abbreviation in Spanish. MATERIAL AND METHODS: Between October 2001 and January 2002, blood samples were collected from voluntary donors at the blood bank of the Hospital General Regional of IMSS in Orizaba; Veracruz, Mexico. The samples were assayed for anti-T. cruzi by ELISA, Western blot and IFI, using a recombinant protein (MBP::Hsp70, and crude extract from epimastigotes. RESULTS: A total of 420 blood donors were studied; two of them were seropositive for ELISA, Western blot and IFI, with a seroprevalence of 0.48%. CONCLUSIONS: Some blood donors at the HGRO hospital were seropositive for T

  15. HBsAg阳性孕妇血清中乙肝病毒前S/S基因的相似株现象%Quasispecies of HBV preS/S gene in HBsAg carrying pregnant women

    Institute of Scientific and Technical Information of China (English)

    李端; 闫永平; 徐德忠; 张景霞

    2001-01-01

    目的探讨HBsAg阳性孕妇体内HBV病毒前S/S基因变异的状况. 方法应用全长PCR技术从4例表面抗原阳性孕妇血清中扩增出HBV全长基因,将其克隆于pUC19载体,每例随机筛选5个阳性克隆,对其前S/S区进行序列分析. 结果 4例患者各克隆间均存在碱基的差异,此类患者体内HBV株同源性较差. 结论 HBsAg阳性孕妇体内HBV株呈“相似株”分布.%AIM To study the mutation of HBV preS/S gene in HBsAg carrying pregnant women. METHODS Full-length polymerase chain reaction (PCR) was used to amplify whole HBV gene from sera of 4 HBsAg carrying pregnant women. The full- length PCR products were cloned into pUC19 vector,5 clones were obtained every woman and subgenomic PCR was used to amplify HBV preS/S gene for DNA sequencing. RESULTS Compared with the wild-type HBV C, all clones exhibited base insertion and substitution. CONCLUSION Quasispecies are the major characteristics in these HBsAg carrying pregnant women.hepatitis B virus; preS/S gene; quasispecies; variation (genetics)

  16. 金标法检测HBsAg漏检原因探讨

    Institute of Scientific and Technical Information of China (English)

    刘赴平; 黄志森; 王德文

    2005-01-01

    随着非固定采血点的无偿献血深入开展,快速金标试纸法筛选HBsAg已经在许多血站得到广泛应用。但由于试纸条的灵敏度和操作方法所限,存在着一定的漏检。为了解漏检原因,笔者对金标法初筛合格的血液,经ELISA法复检出的阳性标本,再用金标法作对比试验,并对漏检原因进行了初步探讨,报告如下。

  17. HBS-1: A Modular Child-Size 3D Printed Humanoid

    Directory of Open Access Journals (Sweden)

    Lianjun Wu

    2016-01-01

    Full Text Available An affordable, highly articulated, child-size humanoid robot could potentially be used for various purposes, widening the design space of humanoids for further study. Several findings indicated that normal children and children with autism interact well with humanoids. This paper presents a child-sized humanoid robot (HBS-1 intended primarily for children’s education and rehabilitation. The design approach is based on the design for manufacturing (DFM and the design for assembly (DFA philosophies to realize the robot fully using additive manufacturing. Most parts of the robot are fabricated with acrylonitrile butadiene styrene (ABS using rapid prototyping technology. Servomotors and shape memory alloy actuators are used as actuating mechanisms. The mechanical design, analysis and characterization of the robot are presented in both theoretical and experimental frameworks.

  18. HBsAg和抗-HCV酶免试剂批检结果分析

    Institute of Scientific and Technical Information of China (English)

    李燕; 徐晓艳; 王欣; 刘虎林

    2003-01-01

    @@ 目前,国家规定对每批试剂都要做好检定工作,以保证血液质量,预防输血后肝炎(PTHC),而对血站来说,选用高质量合格的ELISA诊断试剂是影响检验工作质量的关键因素[1].作者对本站2001年1月~2002年3月HBsAg和抗-HCV酶免试剂检定结果进行分析,希望能为我站选用合格的试剂提供一些有益的参考.

  19. HYDRODYNAMICALLY BALANCED SYSTEMS (HBS: INNOVATIVE APPROACH OF GASTRO RETENTION: A REVIEW

    Directory of Open Access Journals (Sweden)

    Dubey Vivek

    2012-06-01

    Full Text Available The objective of writing this review on hydrodynamically balanced systems (HBS was collection of the recent literature. The design of hydrodynamically balanced drug delivery system is base on prolong GI residence time of drug in an area of the GI tract to maximize drug reaching its absorption. Gastrointestinal transit time of orally administered dosage forms are controlled by using gastro retentive drug delivery systems (GRDDS. Need for GRDDS a controlled drug delivery system with prolonged residence time in the stomach. Floating drug delivery systems is one of the important approaches to achieve gastric retention to obtain sufficient drug bioavailability. This delivery system is desirable for drugs with an absorption window in the stomach or in the upper small intestine.

  20. CEA、AFP、TPA及HBsAg联检在肝癌诊断中的意义

    Institute of Scientific and Technical Information of China (English)

    李红梅; 敬华; 仲人前; 刘春雷; 于嘉屏; 孔宪涛

    2005-01-01

    肝细胞癌(HCC)是最常见的肿瘤之一,甲胎蛋白(AFP)是HCC最重要的肿瘤标志物,但其特异性仍有一定局限性。癌胚抗原(CEA)、组织多肽抗原(TPA)均可高浓度地存在于多种肿瘤病人的血清中,乙肝病毒(HBV)是肝细胞癌的重要致癌因子,它们对HCC的诊断、病情监测均有一定的价值。我们对HCC病人进行了CEA、AFP、TPA及乙肝表面抗原(HBsAg)的联合检测,分析它们的临床意义,现报道如下。

  1. Optimization of in vitro HBV replication and HBsAg production in HuH7 cell line.

    Science.gov (United States)

    Cavallone, Daniela; Moriconi, Francesco; Colombatto, Piero; Oliveri, Filippo; Bonino, Ferruccio; Brunetto, Maurizia Rossana

    2013-04-01

    The Gunther's vector-free method (GM), using PCR-amplified full length HBV-DNA (fl-HBV-DNA), is currently the best in vitro HBV replication system despite the low intracellular HBV-DNA production. The replication efficiency and HBsAg secretion of 12 isolates from HBsAg/HBeAg positive sera by GM, Monomer-Linear-Sticky-Ends-DNA (MLSE) and Monomer-Circular-Closed (MCC) were compared in HuH7 cells. Eight of twelve genomes (67%) were replication competent by GM; however direct sequencing (DS) showed that more than 80% of input DNA was undigested in spite of SapI treatment. Replication Intermediates (RI) were detected earlier (24 vs. 48h) and in higher amounts (2.51±0.32 and 6.43±0.43 fold) by MCC than GM or MLSE. By MCC 10 of 12 genomes (83%) were replication competent and 7 produced high RI levels. RI and HBsAg kinetics correlated positively in MCC (R=0.696, p=0.017 overall; R=0.928, p=0.008), but not in GM (R=-0.437, p=0.179 overall; R=-0.395, p=0.439) in genotype D isolates. In conclusion, HBV-DNA circularization prior transfection improves in vitro viral replication and replication competent HBsAg production, mimicking better the in vivo conditions.

  2. DETERMINACIÓN DEL CATABOLISMO DE LOS ANTICUERPOS MATERNOS Y SU INTERACCIÓN CON DIFERENTES PLANES VACUNALES PARA LA ENFERMEDAD DE GUMBORO EN POLLOS DE ENGORDE

    Directory of Open Access Journals (Sweden)

    Robin O. J.

    2006-11-01

    Full Text Available Con el in de evaluar el catabolismo de los anticuerpos maternos y su interferencia con tres planes vacunales diferentes contra la enfermedad de Gumboro se realizó un estudio utilizando pollos de la estirpe Ross 308, divididos en cuatro grupos: grupo 1: control (sin vacuna; grupo 2: vacunado los días 1, 7 y 15; grupo 3: vacunados los días 1 y 12; y grupo 4: vacunado el día 12. En el primer experimento el biológico se suministró en el agua de bebida, y en el segundo se hizo en el pico. Se sacriicaron 15 aves de cada grupo a los 1, 12, 21 y 42 días de edad y se evaluó peso corporal, tamaño y peso de la bolsa de Fabricio, relación peso bolsa/peso corporal (PB/PC, grado de depleción linfoide, presencia de cepas estándar y/o variantes del virus y los títulos de anticuerpos para la enfermedad de Gumboro. El catabolismo de los anticuerpos maternos ocurrió entre los 21 y 28 días de edad. En los grupos vacunados no se evidenció una respuesta inmune activa frente a ningún plan vacunal. La relación PB/PC no indicó atroia de la bolsa en el experimento 1, aunque las lesiones histopatológicas en el último muestreo fueron grado 3; en el segundo experimento solamente el grupo 3 presentó una disminución en la relación PB/PC; las lesiones histopatológicas en el tercer y cuarto muestreos, en todos los grupos, fueron clasiicadas como grado 3 y 4. En los dos experimentos se detectó la presencia de cepas tanto clásicas como variantes y una baja respuesta humoral. Ninguno de los tres planes vacunales conirió adecuada protección a las aves.

  3. DETERMINACIÓN DEL CATABOLISMO DE LOS ANTICUERPOS MATERNOS Y SU INTERACCIÓN CON DIFERENTES PLANES VACUNALES PARA LA ENFERMEDAD DE GUMBORO EN POLLOS DE ENGORDE

    Directory of Open Access Journals (Sweden)

    R. Castañeda

    2006-01-01

    Full Text Available Con el in de evaluar el catabolismo de los anticuerpos maternos y su interferencia con tres planes vacunales diferentes contra la enfermedad de Gumboro se realizó un estudio utilizando pollos de la estirpe Ross 308, divididos en cuatro grupos: grupo 1: control (sin vacuna; grupo 2: vacunado los días 1, 7 y 15; grupo 3: vacunados los días 1 y 12; y grupo 4: vacunado el día 12. En el primer experimento el biológico se suministró en el agua de bebida, y en el segundo se hizo en el pico. Se sacriicaron 15 aves de cada grupo a los 1, 12, 21 y 42 días de edad y se evaluó peso corporal, tamaño y peso de la bolsa de Fabricio, relación peso bolsa/peso corporal (PB/PC, grado de depleción linfoide, presencia de cepas estándar y/o variantes del virus y los títulos de anticuerpos para la enfermedad de Gumboro. El catabolismo de los anticuerpos maternos ocurrió entre los 21 y 28 días de edad. En los grupos vacunados no se evidenció una respuesta inmune activa frente a ningún plan vacunal. La relación PB/PC no indicó atroia de la bolsa en el experimento 1, aunque las lesiones histopatológicas en el último muestreo fueron grado 3; en el segundo experimento solamente el grupo 3 presentó una disminución en la relación PB/PC; las lesiones histopatológicas en el tercer y cuarto muestreos, en todos los grupos, fueron clasiicadas como grado 3 y 4. En los dos experimentos se detectó la presencia de cepas tanto clásicas como variantes y una baja respuesta humoral. Ninguno de los tres planes vacunales conirió adecuada protección a las aves.

  4. [A retrospective study on HBsAg clearance rate after antiviral therapy in children with HBeAg-positive chronic hepatitis B aged 1-7 years].

    Science.gov (United States)

    Zhu, S S; Dong, Y; Xu, Z Q; Wang, L M; Chen, D W; Gan, Y; Wang, F C; Yan, J G; Cao, L L; Wang, P; Zhang, H F

    2016-10-20

    Objective: To investigate the HBsAg clearance rate after antiviral therapy in children with HBeAg-positive chronic hepatitis B (CHB) aged 1-7 years. Methods: A retrospective analysis was performed for the HBsAg clearance rate in 293 children who were hospitalized in 302 Hospital of PLA from June 2006 to December 2013, met the inclusion criteria, received antiviral therapy, and were followed up for at least 6 months after the withdrawal of antiviral therapy. The t-test or the rank sum test was applied according to the distribution of continuous data, and the chi-square test was used for comparison of categorical data. Results: The HBsAg positive rate of children's mothers was 91.1%. In the age groups of >1-≤2 years, >2-≤3 years, >3-≤4 years, >4-≤5 years, >5-≤6 years, and >6-≤7 years, the HBsAg clearance rates were 66.1%, 65.5%, 45.7%, 41.3%, 20.6%, and 27.6%, respectively. There were significant differences in HBsAg clearance rate between the age groups of >1-≤3 years and >3-≤5 years, >1-≤3 years and >5-≤7 years, and >3-≤5 years and >5-≤7 years (P = 0.001, 0.000, and 0.008). Of all children, 64.8% were boys, among whom 41.1% achieved HBsAg clearance, and 35.2% were girls, among whom 61.2% achieved HBsAg clearance; there was a significant difference in HBsAg clearance rate between boys and girls (P = 0.001). The children with pretreatment alanine aminotransferase (ALT) levels of ≤80 IU/L, > 80 IU/L, ≤200 IU/L, and > 200 IU/L had HBsAg clearance rates of 40.7%, 51.2%, 47.6%, and 49.4%, respectively; there were no significant differences in HBsAg clearance rate between the ALT ≤80 IU/L and ALT > 80 IU/L groups and the ALT ≤200 IU/L and ALT > 200 IU/L groups (P = 0.101 and 0.778). There was no significant difference in HBsAg clearance rate between the pretreatment HBV DNA load clearance rate of 57.1%, and 85% had genotype C and an HBsAg clearance rate of 39.5%; there was no significant difference in HBsAg clearance rate between the

  5. New amino acid changes in drug resistance sites and HBsAg in hepatitis B virus genotype H.

    Science.gov (United States)

    Fernández-Galindo, D A; Sánchez-Ávila, F; Bobadilla-Morales, L; Gómez-Quiróz, P; Bueno-Topete, M; Armendáriz-Borunda, J; Sánchez-Orozco, L V

    2015-06-01

    Long-term treatment with retrotranscriptase (RT) inhibitors eventually leads to the development of drug resistance. Drug-related mutations occur naturally and these can be found in hepatitis B virus (HBV) carriers who have never received antiviral therapy. HBsAg are overlapped with RT domain, thus nucleot(s)ide analogues (NAs) resistance mutations and naturally-occurring mutations can cause amino acid changes in the HBsAg. Twenty-two patients with chronic hepatitis B were enrolled; three of them were previously treated with NAs and 19 were NAs-naïve treated. HBV reverse transcriptase region was sequenced; genotyping and analysis of missense mutations were performed in both RT domain and HBsAg. There was predominance of genotype H. Drug mutations were present in 18.2% of patients. Classical lamivudine resistance mutations (rtM204V/rtL180M) were present in one naïve-treatment patient infected with genotype G. New amino acid changes were identified in drug resistance sites in HBV strains from patients infected with genotype H; rtQ215E was present in two naïve-NAs treatment patients and rtI169M was identified in a patient previously treated with lamivudine. Mutations at sites rt169, rt204, and rt215 resulted in the Y161C, I195M, and C206W mutations at HBsAg. Also, new amino acid changes were identified in B-cell and T-cell epitopes and were more frequent in HBsAg compared to RT domain. In conclusion, new amino acid changes at antiviral resistance sites, B-cell and T-cell epitopes in HBV genotype H were identified in Mexican patients.

  6. Vacinação contra hepatite B e Anti-HBS entre trabalhadores da saúde

    OpenAIRE

    Souza,Fernanda de Oliveira; Freitas,Paloma de Sousa Pinho; de Araújo, Tânia Maria; Gomes,Mariana Rabelo

    2015-01-01

    ResumoObjetivoAvaliar a prevalência de vacinação para hepatite B e os fatores associados entre trabalhadores da atenção primária e da média complexidade do setor saúde.MetodologiaEstudo transversal com 506 trabalhadores do setor saúde de um município do Recôncavo Baiano. Utilizou-se questionário para investigar situação vacinal para hepatite B. Análises uni e bivariada foram utilizadas para avaliação dos fatores associados à vacinação.ResultadosHouve predomínio do sexo feminino (78,3%) e do v...

  7. Significance of application of high-sensitive HBsAg ELISA kits in qualified blood donors determined by routine screening tests%超敏HBsAg ELISA检测试剂在常规检测合格的献血者中应用的意义

    Institute of Scientific and Technical Information of China (English)

    桑列勇; 傅立强; 庄培芬; 方放

    2011-01-01

    Objective;To assess the significance of the application of high - sensitive HBsAag ELISA kits in qualified blood donors determined by routine screening tests. Methods: HBsAg was determined in 8692 blood donors by one imported high - sensitive ELISA kit and two domestical produced ELISA kits, respectively. The results were further confirmed by three nested - PCRs specific for different regions in HBV - DNA and other serologic markers of HBV. Results; 48 out of 8692 blood donors were HBsAg positive. Among them, 32 were detected by Hepanostika? HBsAg ELBA kit, 22 by InTec PRODUCTS kit and 14 by Kehua Bio - engineering kit. The "a" epitope was detected in 40 out of 8692 samples by HBV - DNA analysis. Among those, 13 were determined as HBsAg positive and 8 negative by all three ELISA kits. In another 8 samples, HBsAg could be detected only by high - sensitive Hepanostika? HBsAg ELISA kit. Conclusion: Although the application of high - sensitive HBsAg ELISA kit can significantly decrease the risk of HBV infection in blood transfusion, the possibility of missing detection still remains. Therefore, the current strategy should focus on increasing the sensitivity of ELISA kit and promoting the use of DNA analysis technology. The combination of ELISA determination and DNA analysis can profoundly ensure the safety of blood transfusion.%目的:探讨在无偿献血HBsAg筛查中使用超敏HBsAg ELISA的意义.方法:使用一种进口超敏HBsAg试剂和两种国产HBsAg试剂对8692例无偿献血标本进行平行检测,并使用不同区域的三套巢式PCR和检测乙肝三系其他指标对HBsAg结果进行验证.结果:8692例标本中共检测出HBsAg阳性48例,其中Hepanostika HBsAg ELISA试剂32例,英科新创HBsAg ELISA试剂22例,科华HBsAg ELISA试剂14例,对8692例标本进行HBV-DNA测定,有40例获得a表位基因,其中13例为三种HBsAg ELISA试剂检测均阳性,8例为三种HBsAg ELISA试剂检测均阴性,8例为Hepanostika.HBsAg ELISA试剂检测

  8. Role of quantitative hepatitis B surface antigen in predicting inactive carriers and HBsAg seroclearance in HBeAg-negative chronic hepatitis B patients

    Science.gov (United States)

    Ungtrakul, Teerapat; Sriprayoon, Tassanee; Kusuman, Pattama; Chunnuan, Pitchayachuda; Soonklang, Kamonwan; Sornsamdang, Gaidganok; Auewarakul, Chirayu U.; Tanwandee, Tawesak

    2017-01-01

    Abstract To evaluate quantitative hepatitis B surface antigen (qHBsAg) as a diagnostic marker for inactive carriers (ICs) and hepatitis B surface antigen (HBsAg) seroclearance in hepatitis B e antigen (HBeAg)-negative chronic hepatitis B (CHB) patients. We retrospectively studied 300 HBeAg-negative CHB patients with initial serum hepatitis B virus (HBV) Deoxyribonucleic acid (DNA) levels <2000 IU/mL. Serum HBV DNA and alanine aminotransferase (ALT) levels were monitored every 6 months for 24 months. ICs were identified as having persistent HBV DNA levels <2000 IU/mL and normal ALT levels, whereas active carriers (ACs) were identified as having HBV DNA levels ≥2000 IU/mL, with or without elevated ALT levels. The serum qHBsAg level was defined at baseline and evaluated as a diagnostic predictor using a receiver-operating characteristic curve. The study group comprised 134 men and 166 women with a median age of 41.5 years. At baseline, 200 ICs displayed lower levels of qHBsAg (1492 IU/mL) compared with 100 ACs (2936 IU/mL) (P = 0.005). The qHBsAg level was independently associated with the IC state and HBsAg seroclearance. Baseline qHBsAg levels <1000 IU/mL and HBV DNA levels <2000 IU/mL, when detected simultaneously, allowed for identification of ICs with 41% sensitivity and 72% specificity. Fifteen patients (5%) displayed HBsAg seroclearance after 24 months. A qHBsAg cutoff value of <50 IU/mL provided 100% sensitivity and 92% specificity in predicting HBsAg seroclearance. The qHBsAg level at a single timepoint among HBeAg-negative CHB patients with low HBV DNA levels at baseline was not a predictive marker for ICs; however, it accurately predicted spontaneous HBsAg seroclearance at 24 months. PMID:28353619

  9. The Comparison of Detection of HBsAg of Blood - donation Volunteers between ELISA Method and Gold Test Strip Method%ELISA法与金标试纸法检测无偿献血者HBsAg的比较

    Institute of Scientific and Technical Information of China (English)

    汪青山

    2010-01-01

    目的 研究金标试纸法与ELISA法检测无偿献血者HBsAg结果的阳性率和漏检率.方法 我站收集的18000份样本分别用金标试纸法与ELISA法测试HBsAg,比较两种方法的阳性率.结果 金标试纸检出的阳性与ELISA法检出的阳性完全相符,另外ELISA法检出的134份阳性标本为金标试纸法漏检.结论 金标试纸法漏检率高于ELISA法.

  10. 健康教育路径对HBsAg阳性产妇母乳喂养依从性的影响%Effects of Health Education Path on Breastfeeding Compliance of HBsAg Positive Puerperae

    Institute of Scientific and Technical Information of China (English)

    尤炜; 轧春妹; 李楠

    2012-01-01

    Objective To investigate the effects of health education path on breastfeeding compliance of HBsAg positive puerperae.Methods Using convenience sampling method, 238 HBsAg positive puerperae from January 2009 to January 201.1 were selected as observational group to receive health education path.263 HBs/Vg positive puerperae from December 2006 to December 2008 were selected as control group to receive, routine health education.The inter-group maternal breastfeeding compliance and awareness of maternal breastfeeding knowledge were compared.Results Compared with the control group, the awareness of maternal breastfeeding knowledge was significantly higher and 70.b9% oi the puerperae gained 80 points or higher( P < 0.01).Compared with the control group, 69.75% of the puerperae' breastfeeding time are more than six months,19.75% are less than six months.only 10.50% without breastfeeding in the observational group, and the differences are significant ( P <0.01).Conclusion Breastfeeding of HBsAg-positive mothers does not increase the HBV infection of infants.Health education path can effectively improve the compliance of maternal breastfeeding and awareness of feeding knowledge.%目的 探讨健康教育路径对HBsAg阳性产妇母乳喂养依从性的影响.方法 便利抽样法选择2009年1月至2011年1月住院分娩的238例HBsAg阳性产妇为观察组,采用健康教育路径方式宣教;选择2006年12月至2008年12月住院分娩的263例HBsAg阳性产妇为对照组,采用传统健康教育方式进行宣教.比较两组产妇母乳喂养依从性及产妇母乳喂养知识知晓情况.结果 观察组产妇对于母乳喂养的相关知识知晓情况较好,得分80分以上的占70.59%,与对照组相比,差异有统计学意义(P<0.001).观察组中,哺乳≥6个月的产妇占69.75%,哺乳<6个月者占19.75%,未采用母乳喂养者仅占10.50%,与对照组相比,差异有统计学意义(P<0.01).结论 HBsAg阳性产妇进行母乳喂养并不增加婴

  11. Application of Shewhart-cusun Control Chart in the Internal Quality Control of ELISA Detecting HbsAg%Shewhart-cusum质控图在酶联法检测HBsAg室内质控中的应用

    Institute of Scientific and Technical Information of China (English)

    夏勇; 吴宗华; 谭佩仪; 林丽英

    2010-01-01

    目的 探讨ELISA法检测HBsAg的室内质控方法 .方法 用ELISA法检测0.5 ng/ml的HBsAg质控血清,采用Shewhart-cusum联合质控图制作质控图,结果 检测浓度为0.5 ng/ml的HBsAg质控品的s/co均值为1.879,s为0.205,告警限k为均值士1s,分剐是1.674和2.084,失控限h为均值±2.7s,分别是2.433和1.325.结论 Shewhartcusum联合质控图对于容易出现随机误差和系统误差的定性ELISA方法 较为适用,并且准确区分随机误差和系统误差有助于临床实验室采取相应的措施有针对性的进行改正.

  12. [Prevalence of HBs antigen carriage in a population of national Gendarmerie recruits in Ivory Coast in 2008].

    Science.gov (United States)

    Kra, O; N'dri, N; Ouattara, B; Kadjo, K; Aba, T; Bissagnéné, E

    2012-01-01

    The purpose of this study was to determine the prevalence of HBsAg carriage in Ivorian gendarmerie recruits in 2008. This prevalence was 15.6%. It was highest in recruits aged 18 years (24%), in those who were married (33.3%) and in those who had had more than three sexual partners in the 6 months before the survey (19.4%). It was also higher in recruits who had never used condoms (20.6%) and in those who had undergone blood transfusions (27%). A policy of vaccination against HBV in the army, in teenagers and adults must be implemented to reduce HBsAg carriage in the army and in the Ivorian population.

  13. High brilliant thermal and cold moderator for the HBS neutron source project Jülich

    Science.gov (United States)

    Cronert, T.; Dabruck, J. P.; Doege, P. E.; Bessler, Y.; Klaus, M.; Hofmann, M.; Zakalek, P.; Rücker, U.; Lange, C.; Butzek, M.; Hansen, W.; Nabbi, R.; Brückel, T.

    2016-09-01

    The proposed High Brilliance Neutron Source (HBS), recognized within the Helmholtz Association of German Research Centres, will optimize the entire chain from particle source through particle accelerator, target, moderator, reflector, shielding, beam extraction, beam transport all the way to the detector, utilizing the nuclear Be(p,n) or Be(d,n) reaction in the lower MeV energy range. A D2O moderating reflector prototype (MRP) and a cold source were constructed and build according to MCNP parameter studies. The MRP was tested in a feasibility study at the TREFF instrument at MLZ (Garching). Cold beam extraction from the flux maximum within the moderator based on liquid para H2 and other cold moderators will be tested by energy spectroscopy via TOF-method. Different ratios of liquid ortho/para H2 will be fed to the cold moderator. The ratio will be controlled by feeding from reservoires of natural liquid H2 and a storage loop with an ortho/para converter and determined via online heat capacity measurement.

  14. ASSOCIATION OF HBsAG WITH SEVERE MALARIA - FACT OR FICTION?

    Directory of Open Access Journals (Sweden)

    Anup K

    2012-12-01

    Full Text Available ABSTRACT: India lies in the endemic belt of PF malaria. Annua lly we are faced with regular outbreaks of severe malaria with its attendent adve rse effects on public health in the North-East also. Owing to the diverse geographic, sociopolitical , economic, ethnic and cultural characteristics, this region is also ideal for futu re “epidemics” of potentially dangerous consequences of HBV infection e.g in Arunachal Prad esh in particular. Epidemiologically, a significant serological association between HbsAg po sitivity and severe malaria has been reported recently from Africa(,Gambia. But, the mul tiple factors predisposing to severe malaria, and those influencing the progression of HB V infection have not been elucidated properly. It was postulated that the liver stage parasi tes are not properly cleared due to concurrent HBV infection of the hepatocytes. Inducib le Genetic mutations protective in nature may be a factor. Similarly, those who are vaccinate d against HBV are reported to suffer from malaria in South Asia. However, it is premature to d raw any definite conclusions as our personal experience give us results to the contrary.

  15. Characterisation and follow-up study of occult hepatitis B virus infection in anti-HBc-positive qualified blood donors in southern China

    Science.gov (United States)

    Ye, Xianlin; Li, Tong; Xu, Xiaoxuan; Du, Peng; Zeng, Jinfeng; Zhu, Weigang; Yang, Baocheng; Li, Chengyao; Allain, Jean-Pierre

    2017-01-01

    Background Most major Chinese blood centres look for hepatitis B surface antigen (HBsAg) and perform nucleic acid testing to screen blood for hepatitis B virus infection. The search for antibodies to the core of hepatitis B virus (anti-HBc) has not been implemented because it would lead to a high rate of discarded blood units. The aim of this study was to evaluate the prevalence of occult HBV infection among anti-HBc-positive qualified blood donors in southern China. Materials and methods We tested anti-HBc-positive blood donations negative for HBsAg and HBV DNA by standard NAT from Shenzhen for the presence of HBV DNA by sensitive nested and quantitative polymerase chain reactions. Anti-HBs titres were quantified. HBV DNA-positive donors were traced and followed-up. Results Of the 1,033 qualified donors, 47.4% (95% CI: 44.4 to 50.5%) carried anti-HBc as evidence of exposure to HBV. The rate of anti-HBc positivity increased steadily with age, ranging from 32.6% in the age group 10 IU/L). HBV DNA was detected in 14 donors who were anti-HBc-positive, HBsAg-negative and negative by routine NAT. Seven of those 14 specimens had an anti-HBs titre above 100 mIU/mL. The prevalence of OBI in anti-HBc-positive qualified blood donors was 2.86% (95% CI: 1.57 to 4.75%). Eight of the 14 OBI cases were genotype B and one was genotype C; 7/14 cases were followed-up, one case converted to anti-HBe. HBV DNA became undetectable in all follow-up samples. Discussion A small proportion of anti-HBc-positive qualified donors carry HBV DNA after HBsAg and NAT screening. This finding suggests the possibility of HBV transmission from asymptomatic donors, especially in areas of high HBV prevalence. More sensitive NAT rather than anti-HBc testing should be considered to improve blood safety. PMID:27416568

  16. Tissue localization of C1q in HBs antigen positive liver disease patients by direct immunofluorescent technique

    Directory of Open Access Journals (Sweden)

    Tsuji,Takao

    1977-02-01

    Full Text Available Tissue localization of a subcomponent of the first component of complement (CLq was examined in one postmortem case of HBs antigen (HBs Ag positive hepatocellular carcinoma and in six cases of chronic hepatitis from liver biopsy specimens. The direct immunofluorescent method was used after fixation with 2% para-formaldehyde in concentrated ammonium sulfate. CLq localization was found in collagen fibers and the cytoplasm of fibroblasts in the connective tissues of specimens examined. The localization was particularly marked in the region of the fundal glands of the gastric wall. Apart from collagen fibers, other sites of localization included the surface membrane of lymphocytes, especially those cells of the mesenteric lymph nodes. In HBs Ag positive specimens, immune deposit-like substances appeared localized intra-hepatically and in the renal glomeruli. Since C3 and C4 were identified concomitantly, it indicates that these substances were indeed immune diposits. Despite the finding that C3 and C4 were identified together in the hepatic cell cytoplasm, C1q itself was not demonstrated in all hepatic cell cytoplasms.

  17. Complement fixing hepatitis B core antigen immune complexes in the liver of patients with HBs antigen positive chronic disease.

    Science.gov (United States)

    Rizzetto, M; Bonino, F; Crivelli, O; Canese, M G; Verme, G

    1976-01-01

    One hundred and fifty-two biopsies from serologically HBsAg positive and negative patients with liver disease were studied in immunofluorescence: for the presence of the surface (HBs) and the core (HBc) antigenic determinants foeterminants of the hepatitis B virus, of immunoglobulins and complement (C) deposits, and for the capacity to fix human C. Circumstantial evidence is presented suggesting that HBc immune-complexes are a relevant feature in the establishment and progression of chronic HBSAg liver disease. C fixation by liver cells was shown in all HBC positive patients with chronic hepatitis; an active form was present in every case, except two with a persistent hepatitis, an inverse ratio of HBc to C binding fluorescence being noted between active chronic hepatitis and cirrhotic patients. HBc without C fixation was observed in only three patients in the incubation phase of infectious hepatitis. IgG deposits were often found in HBc containing, C fixing nuclei. No C binding or IgG deposits were observed in acute self-limited type B hepatitis, in serologically positive patients with normal liver or minimal histological lesions, with and without HBs cytoplasmic fluorescence in their biopsy, or in serologically negative individuals. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:1001973

  18. 重组人源性抗HBsAg Fab抗体的肽质量图谱分析

    Institute of Scientific and Technical Information of China (English)

    邓宁; 向军俭; 唐勇; 王宏

    2005-01-01

    重组人源性抗HBsAg Fab抗体具有较好的特异性和抗原结合活性,为了更好的阐明毕赤酵母表达的重组人源性抗HBsAg Fab抗体的性质,用基质辅助激光解析飞行时间质谱(MALDI-TOF-MS)对重组Fab抗体的分子质量和肽质量图谱进行了分析。结果显示,毕赤酵母表达的重组人源性抗HBsAg Fab抗体的分子质量为50678.49Da,与根据其一级结构计算的理论分子质量相比多2763.84Da,显示酵母表达的重组Fab抗体为糖蛋白。用胰蛋白酶酶解重组Fab抗体后进行MALDI-TOF-MS分析显示,大部分的酶解肽段均能检测出来。结果表明毕赤酵母表达的重组Fab抗体与预期的结构一致。

  19. 两种检测方法在HbsAg低浓度标本检测结果的分析%Two Kinds of Detection Methods Analyzing the Results of HbsAg low Con-centration Samples

    Institute of Scientific and Technical Information of China (English)

    刘鹏

    2015-01-01

    目的:对于化学发光磁微粒子免疫分析法(CMIA)和酶联免疫吸附法(ELISA)在乙肝表面抗原检测中低浓度标本结果对比分析。方法先用时间分辨免疫荧光法(TRFIA)检测并确定HbsAg低浓度标本(0.5~1.0 ng/mL)236份和HbsAg阴性标本(约0.5 ng/mL)100份。分别使用CMIA和ELISA进行检测,对检测结果进行对比分析。结果ELISA法检测出阳性标本为195例,阳性率为82.6%。CMIA法检测出阳性标本为232例,阳性率为98.3%。差异有统计学意义(χ2=33.62,P0.05)。结论CMIA法敏感度和特异性均高于ELISA法,建议在HbsAg的检测尤其是低浓度标本的检测中使用高敏感度与高特异性的方法。%Objective To compare and analysis the results for chemiluminescent magnetic micro particle immunoassay (CMIA) and enzyme-linked immunosorbent assay (ELISA) in low concentration samples of hepatitis B surface antigen detection. Methods Hb-sAg low concentration (0.5~1.0 ng/mL) 236 and HbsAg negative samples (0.5 ng/mL) 100 were detected by time resolved im munofluorescence assay (TRFIA). CMIA and ELISA were used to detect and compare the results. Results 195 positive samples were detected by ELISA, the positive rate was 82.6%. 232 cases positive samples were detected by CMIA method, the positive rate was 98.3%. The two groups was statistically significant (χ²=33.62,P0.05). Conclusion The sensitivity and specificity of MIA were higher than ELISA, and it was suggested that the method of high sensitivity and specificity in the detection of HbsAg was used, especially in low concentration samples.

  20. Evaluation of Domestic Chemiluminescence Immunoassay Kit as a Potential Application in the Detection of HBsAg%国产化学发光免疫试剂检测血清HBsAg的应用评价

    Institute of Scientific and Technical Information of China (English)

    张雄民; 伍晓菲; 刘宇宁; 孟妍; 贾尧; 王迅

    2009-01-01

    目的 考核国产化学发光免疫分析试剂检测血清HBsAg的效果,探讨其在血液筛查和临床检测应用中的可行性.方法 采用国产化学发光免疫分析试剂筛查345份HBsAg确认阳性的血清标本,比较其与常用的进口和国产酶联免疫检测试剂盒的漏检率;并采用不同浓度的标准HBsAg血清对国产化学发光免疫分析试剂进行线性分析和精密性试验.结果 国产化学发光免疫分析检测试剂的漏检率高于Murex和Organon酶联免疫试剂盒,差异有统计学意义(P<0.01),而与国产英科新创(厦门)科技公司和上海科华生物技术有限公司的HBsAg酶联免疫诊断试剂盒的差异无统计学意义;采用标准血清进行线性分析.标本浓度在(0.5~150)ng/ml范围时与化学发光单位量(RLUs)呈良好的线性正相关;分别对低、中、高浓度标准血清进行板内和板间的重复检测,其反应具有良好的重复稳定性.结论 化学发光免疫分析法检测血清HBsAg具有快速、可定量、反应稳定的特点.可用于血液筛查和临床标本的检测.

  1. Bajo índice de sospecha para deficiencia de anticuerpos en niños sometidos a procedimientos quirúrgicos de Otorrinolaringología

    Directory of Open Access Journals (Sweden)

    AB Ramírez-López

    2016-06-01

    Full Text Available INTRODUCCIÓN: las inmunodeficiencias primarias están subdiagnosticadas en todo el mundo, aun en centros de tercer nivel de atención. Los defectos de anticuerpos constituyen el grupo más prevalente y suelen manifestarse clínicamente después de los 6 meses de edad como infecciones respiratorias recurrentes causadas por bacterias encapsuladas. Algunas cirugías de oído-nariz y garganta se indican en pacientes con infecciones respiratorias recurrentes o complicadas que no han respondido adecuadamente al tratamiento médico. Estos niños sometidos a procedimientos quirúrgicos luego de una historia de infecciones respiratorias recurrentes pueden constituir un grupo de alto riesgo para inmunodeficiencias primarias.   OBJETIVO: indagar qué tan frecuentes son los defectos de anticuerpos en niños sometidos a cualquiera de tres cirugías de otorrinolaringología.   MATERIALES Y MÉTODOS: revisamos los expedientes electrónicos de niños que hubieran sido sometidos a adenoamigdalectomía (ATT, drenaje endoscópico de senos paranasales (ESD y colocación de tubos timpánicos de ventilación (TVT en nuestro instituto durante 2011-2012, en busca de niveles séricos de inmunoglobulinas (IgE, IgG, IgA, IgM.   RESULTADOS: encontramos 112 procedimientos quirúrgicos en 87 pacientes, de los cuales la indicación para cirugía fue infecciosa en 37 (21 varones, 1 muerto, edad media 7.3 años, más frecuentemente rinosinusitis (19/37 y amigdalitis (9/37 crónicas. Los procedimientos incluyeron: 24 adenoamigdalectomías, 13 drenajes endoscópicos de senos paranasales y 6 colocaciones de tubos timpánicos de ventilación, para un total de 43; ocho pacientes (21.6% fueron sometidos a más de una cirugía. La IgE sérica se encontró en el expediente de 27 pacientes (72.9%, y “al menos IgG” en 18 (48.6%. Sólo 70% de las pruebas fueron ordenadas antes de la cirugía. Los resultados anormales incluyeron: IgE elevada en 10/27, Ig

  2. Asociación entre anticuerpos antifosfolípidos y complicaciones de la gestación en mujeres de Costa Rica

    Directory of Open Access Journals (Sweden)

    Roubier Rojas-Barahona

    2007-04-01

    Full Text Available Objetivo: Determinar asociación entre los anticuerpos antifosfolípidos y complicaciones de la gestación (aborto de cualquier tipo, preeclampsia, eclampsia, insuficiencia placentaria grave o parto antes de la semana 34 de gestación, tomando como base la población de pacientes del servicio de Obstetricia del Hospital México. Materiales y métodos: Estudio de casos y controles en la población de pacientes atendidas por el servicio de Obstetricia del Hospital México en el período comprendido entre enero de 2002 y diciembre de 2004. Se tomaron los datos del registro de egresos Hospitalarios, se hizo un análisis descriptivo, en análisis univariado y multivariado de las principales variables registradas. Resultados: El promedio de edad de las pacientes en los casos y los controles no mostró diferencia significativa. La provincia de San José reporta el mayor porcentaje de casos con un 39.9%, seguido por Heredia con un 26.8% y Alajuela con un 24.6%. En cuanto al número de gestaciones, se determinó que no influyen significativamente en relación con el aborto y otras complicaciones de la gestación. El análisis univariado y multivariado mantiene asociación entre los antecedentes clínicos de las gestaciones anteriores, además de los anticuerpos antifosfolípidos respecto al aborto y otras complicaciones de la gestación. Conclusión: Los anticuerpos antifosfolípidos deberían ser tomados en cuenta como parte del seguimiento del embarazo en pacientes con historia de abortos y complicaciones de la gestación en embarazos previos.Aim: To determine the association between antiphospholipid antibodies and pregnancy failures (abortion, preclampsia, eclampsia, placental insufficiency or delivery before the 34th week of gestation in Costa Rican women. Materials and Methods: The present is a case-control study, the participants belong to the Obstetrics Department of the Hospital Mexico. The period of the study was from January 2002 to December

  3. Hepatitis B virus surface antigen and anti-hepatitis C virus rapid tests underestimate hepatitis prevalence among HIV-infected patients

    DEFF Research Database (Denmark)

    Hønge, Bo Langhoff; Jespersen, S; Medina, C

    2014-01-01

    to detect HBV surface antigen (HBsAg) and anti-HCV among HIV-infected patients in Guinea-Bissau. METHODS: Blood samples from HIV-infected patients in Guinea-Bissau were stored after testing for HBsAg and anti-HCV with rapid tests. Samples were subsequently re-tested for HBsAg and anti-HCV in Denmark....... RESULTS: Two rapid tests were used in Guinea-Bissau: HBsAg Strip Ref 2034 (VEDA.LAB, Alençon, France; sensitivity 62.3%; specificity 99.2%) and HEPA-SCAN (Bhat Bio-Tech, Bangalore, India; sensitivity 57.1%; specificity 99.7%). In the two tests the ability to obtain the correct outcome depended...... on the antigen and antibody concentrations, respectively. Sex, age, CD4 cell count and antiretroviral therapy status did not differ between false negative and true positive samples in either of the tests. The study is limited by a low number of anti-HCV positive samples. CONCLUSIONS: New diagnostic rapid tests...

  4. 献血人群血清anti-HBC和HBV DNA检测的意义

    Institute of Scientific and Technical Information of China (English)

    朱紫苗; 谢步飞

    2014-01-01

    目的 探讨无偿献血者乙型肝炎病毒核心抗体与隐匿性感染的关系.方法 收集无偿献血者样本9 100份,采用ELISA法进行HBsAg和anti-HBC血清学筛查,对anti-HBC阳性血清PCR检测乙肝病毒核酸.结果 911份(10.01%)标本为anti-HBC阳性,其中820份(90.01%)HBsAg阴性,34例血清HBV DNA阳性.结论 常规检测对于献血者筛查具有重要意义.

  5. Effect of hepatitis B vaccine combined with hepatitis B immune globulin on infection in newborn infants of HBsAg-positive mothers

    Institute of Scientific and Technical Information of China (English)

    Si-Jia Yang; Jin-Hong Liu; Xue-Lian Tong; Ming Chen

    2016-01-01

    Objective:To investigate the effect of hepatitis B vaccine combined with hepatitis B immune globulin on infection in newborn infants of HBsAg-positive mothers. Methods:Two hundred newborn infants of HBsAg-positive mothers who had received prenatal examination and given birth to their child were selected as the study subjects and divided into the control group and the observation group in accordance with the voluntary principle. Newborn infants in the control group received 100 IU of vaccinations of hepatitis B vaccine and hepatitis B immune globulin at postnatal 24 h, 1 month and 6 months, while lying-in women in the observation group were continuously given 200 IU of immune globulin injection at the 27th, 30th, 33rd and 36th weeks of pregnancy. The infection condition and the influence of different delivery modes in infants of the two groups would be observed 12 months after birth. Results:The HBsAg positive rate and HBsAb positive rate of infants in the observation group were 1.00%and 59.00%respectively, while the control group’s were 10.00%and 73.00%. The HBsAg positive rate and HBsAb positive rate of infants in the observation group were significantly lower than those of the control group. The data showed statistical significances. Seven cases of infants born vaginally showed positive HBsAg. One of them belonged to the observation group and the other six were in the control group. Four cases of infants delivered by cesarean section showed positive HBsAg and they all belonged to the control group. The HBsAg positive rate of the observation group was obviously lower than that of the control group no matter what deliver mode those children had received. Conclusions:Hepatitis B vaccine combined with hepatitis B immune globulin could effectively decrease the morbidity rate of positive HBsAg for newborn infants no matter what birth way they had received.

  6. Serum soluble interleukin-2 receptor levels in patients with chronic hepatitis B virus infection and its relation with anti-HBc

    Institute of Scientific and Technical Information of China (English)

    Ping Xiao; Qing-Feng Chen; Yan-Ling Yang; Zhen-Hua Guo; Hong Chen

    2006-01-01

    AIM: To investigate the relationship between serum soluble interleukin-2 receptor (sIL-2R) level and anti-HBc in patients with chronic hepatitis B virus (HBV) infection.METHODS: Sera from 100 patients with chronic HBV infection and 30 healthy controls were included in this study. The patients were divided into group A [HBsAg (+),HBeAg (+) and anti-HBc (+),n = 50] and group B [HBsAg(+), HBeAg (+) and anti-HBc (-), n = 50]. sIL-2R levels were determined using ELISA. HBV DNA and alanine aminotransferase (ALT) were also detected.RESULTS: Serum sIL-2R levels were significantly higher in patients with chronic HBV infection than in healthy controls. Moreover, serum sIL-2R levels were significantly higher in patients with HBsAg (+), HBeAg (+) and antiHBc (+) (976.56±213.51×L03 U/L) than in patients with HBsAg (+), HBeAg (+) and anti-HBc (-) (393.41 ± 189.54×103 U/L, P<0.01). A significant relationship was found between serum sIL-2R and ALT levels (P< 0.01) in patients with chronic HBV infection, but there was no correlation between sIL-2R and HBV DNA levels. The anti-HBc status was significantly related to the age of patients (P< 0.0t).CONCLUSION: The high sIL-2R level is related to positive anti-HBc in chronic hepatitis B patients. Positive anti-HBc may be related to T-lymphocyte activation and negative anti-HBc may imply immune tolerance in these patients.

  7. Determinación de Anticuerpos IgM contra Virus dengue partir de sangre absorbida en papel filtro: Un método alternativo y sencillo

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    María García M

    2000-01-01

    Full Text Available Objetivo: Se propone como alternativa la obtención de muestras de sangre total en papel filtro, para la determinación de anticuerpos IgM contra dengue, por ser un método de recolección sencillo y no requerir de muchos cuidados en el envío al laboratorio. Materiales y métodos: De 100 pacientes con diagnóstico clínico de dengue clásico, se obtuvieron en forma simultánea, muestras de suero en tubos al vacío y de sangre total en papel filtro. Ambas muestras fueron evaluadas con el método serológico ELISA Captura de IgM a los 30 días de obtenida la muestra. Resultados: De las 100 muestras 25 fueron positivas y 75 negativas en suero, y 24 positivas y 74 negativas en papel filtro. Se obtuvo una concordancia por índice Kappa de 0,97, sensibilidad y especificidad de 96,0% y 98,0% respectivamente; el valor predictivo positivo fue 96,0%, y valor predictivo negativo fue 98,0%. Conclusión: Se evidencia muy buena sensibilidad, especificidad y concordancia en la determinación de IgM contra dengue, al utilizar papel filtro en la obtención de muestra de sangre.

  8. Analysis on HBV DNA copies in the serum and breast milk of parturients with HBsAg positive%HBsAg阳性产妇血清和乳汁中HBV DNA载量分析

    Institute of Scientific and Technical Information of China (English)

    邹军; 汪永强

    2015-01-01

    Objective To study the relationship and correlation between serum HBV DNA and breast milk HBV DNA of HBsAg(+) parturients, and to provide evidence of guiding breast feeding for women infected by chronic hepatitis B virus (HBV). Methods A total of 148 parturients with HBsAg(+) of HBV were divided into the four groups according to the copies of HBV DNA in serum:<420 IU/ml group, 420~104 IU/ml group, 105~106 IU/ml group, 107~108 IU/ml group. The HBV DNA copies in the serum and breast milk from the four groups of women were detect-ed by fluorescence quantitative PCR (FQ-PCR) technique respectively. And the relationship and correlation of the load of HBV DNA were performed between in serum and breast milk from the four groups. Results The HBV positive rate of women with HBsAg (+) was 31.8%(47/148) in serum and 16.2%(24/148) in breast milk (χ2=7.355, P<0.05). In the parturients with serum HBV DNA<420 IU/ml, the corresponding breast milk was negative for the expression of HBV DNA. In the women with serum HBV DNA of 420~104 IU/ml, 105~106 IU/ml, 107~108 IU/ml, the corresponding HBV DNA positive rate in breast milk was 14.3%(3/18), 71.4%(5/7) and 84.2%(16/19), respectively (χ2=20.88, P<0.05). When serum HBV DNA load increased, the content of breast milk increased accordingly, which showed a mod-erate correlation (r=0.628, P<0.05). Conclusion HBV DNA is detected in breast milk of HBsAg(+) parturients. Its content is lower than that in the corresponding serum, and it increases when the load of HBV DNA in serum increases, which indicates that baby may be get infected through breast feeding.%目的:探讨HBsAg(+)产妇血清和乳汁中HBV DNA载量关系及相关性,为慢性乙型肝炎产妇母乳喂养提供实验依据。方法对148例HBsAg(+)产妇血清和乳汁中乙肝病毒采用实时荧光定量PCR (FQ-PCR)方法检测HBV DNA,按产妇血清HBV载量分为HBV DNA载量<420 IU/ml组、420~104 IU/ml组、105~106 IU/ml组、107~108 IU/ml组,并同

  9. Los complejos Chaperonina(MSP63inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica.

    Directory of Open Access Journals (Sweden)

    Juan Marzoa

    2009-08-01

    Full Text Available Alteration of the native structure of antigens can lead to the loss of protective epitopes. Our previous results showed that separation of the meningococcal outer membrane proteins in native conditions revealed the existence of protein complexes that could be relevant for the development of new vaccine formulations. The aim of this work was to analyse the immunogenic characteristics of a highly conserved 700 kDa chaperonin complex (CxChap detected and purified by using high resolution clear native electrophoresis. Analysis of the anti-CxChap serum by Western-blotting revealed the presence of antibodies against the MSP63 but also against the macrophage infectivity potentiator-like protein (MIP, which is coopurified with the chaperonin complex. Antibodies raised by immunisation with CxChap chaperonin complex show bactericidal and opsonophagocytic activity.

  10. MicroRNA-122 as a predictor of HBsAg seroclearance in hepatitis B and C dual infected patients treated with interferon and ribavirin

    Science.gov (United States)

    Yen, Yi-Hao; Huang, Chao-Min; Wei, Kuo-Liang; Wang, Jing-Houng; Lu, Sheng-Nan; Lee, Chuan-Mo; Hung, Chao-Hung; Chen, Chien-Hung; Tseng, Po-Lin; Chang, Kuo-Chin; Tsai, Ming-Chao; Lin, Ming-Tsung; Wu, Cheng-Kun; Yang, Cheng-Hong; Moi, Sin-Hua; Cho, Chung-Lung; Hu, Tsung-Hui

    2016-01-01

    It has been demonstrated that microRNA-122 (miR-122) plays key roles in the modulation of hepatitis B virus (HBV) replication. This study examined the role of miR-122 in patients with hepatitis C virus (HCV)-HBV dual infection with active hepatitis C who received pegylated interferon-α and ribavirin dual therapy. We enrolled 121 patients with HCV-HBV dual infection after dual therapy. Stored serum was collected before treatment. RT-PCR was used to analyze miR-122. HBsAg seroclearance was noted in 37 (30.1%) cases during a median follow-up period of 5.4 years. miR-122 was significantly lower in HBsAg seroclearance patients than in non-HBsAg seroclearance patients (P  100 IU/mL versus ≤100 IU/mL (P < 0.001). We concluded that in patients with HBV-HCV dual infection with active hepatitis C, miR-122 was associated with HBsAg seroclearance after therapy and qHBsAg level before therapy, indicating that miR-122 plays key roles in modulating HBV replication. PMID:27665934

  11. Incidência pós-transfusional do HBsAg em crianças com doenças neoplásicas Post-transfusional incidence of HBsAg in children with neoplastic diseases

    Directory of Open Access Journals (Sweden)

    Maria Zélia Fernandes

    2009-04-01

    Full Text Available Há 350 milhões de indivíduos cronicamente infectados pelo vírus da hepatite B (VHB. O desenvolvimento e a gravidade dessa infecção dependem de vários fatores, tais como a idade da primoinfecção e resposta imune, sendo o risco dessa cronificação menor que 5% em adultos e maior que 90% em neonatos. Indivíduo cronicamente infectado pelo VHB durante a infância tem 25% de chance de morrer por cirrose ou hepatocarcinoma. Tais evidências conduziram os autores a desenvolver este estudo cujo objetivo foi estimar a incidência pós-transfusional do antígeno de superfície do vírus da hepatite B (HBsAg em crianças com neoplasias, que foram transfundidas no curso do tratamento ou seguimento da doença. Foi realizado um estudo retrospectivo, com revisão de 333 prontuários do serviço de oncologia do HIVS, de janeiro de 1993 a janeiro de 2005. Adotaram-se como critérios de inclusão: idade menor que 16 anos, diagnóstico de doença neoplásica e realização da pesquisa do HBsAg. Assim, 199 prontuários foram excluídos por não preencherem tais critérios, restando 134 que foram analisados quanto à realização de hemotransfusão. Das 134 crianças, 116 foram transfundidas e 18 não. Apresentaram pesquisa reativa para o HBsAg 32,8% das transfundidas e apenas 5,6% das não-transfundidas. O teste Exato de Fisher mostrou que houve significância estatística (p = 0,023 e observou-se que as proporções de pacientes com pesquisa reativa diferem entre os transfundidos e os não-transfundidos. O odds ratio de um paciente transfundido apresentar pesquisa reativa para o HBsAg foi calculado em 8,28 vezes maior do que um não-transfundido.There are 350 million individuals infected by the hepatitis B virus (HBV. The development and the severity of the infection depend on several factors, such as: age at the first infection and the immunity response of the subject. The risk of chronic infection is less than 5% for adults and greater than 90% for

  12. Influence factors of quantitative changes of dendritic cells in neonate born to HBsAg positive mother%HBsAg阳性母亲新生儿树突状细胞数量变化的影响因素

    Institute of Scientific and Technical Information of China (English)

    郭健; 高怡; 郭珍; 付振东; 郝海燕; 汪波; 王素萍

    2012-01-01

    Objective To investigate the influence factors of quantitative changes of dendritic cells (DC) in neonate born to HBsAg positive mother.Methods Sixty HBsAg positive mothers and their newborns were enrolled from the Third People's Hospital of Taiyuan from July 2011 to March 2012.The serum hepatitis B virus (HBV) markers and HBV DNA in mothers and newborns before vaccination were determined by chemiluminescence immunoassay (CLIA) and fluorescence quantitative polymerase chain reaction (PCR).The circulating frequencies of DC subsets were determined in the newborns by flow cytometry (FCM).The comparison of data was done by Mann-Whitney test and t test.The correlation analysis was done by Spearman rank correlation analysis and chi square test.Results Among 60 newborns,5 were HBsAg positive and HBV DNA negative.Among 60 HBsAg positive mothers,21 were HBeAg positive and 29 were HBV DNA positive.There was no significant quantitative difference of neonatal myeloid dendritic cells (mDC) and plasmacytoid dendritic cells (pDC) between intrauterine infection group and intrauterine non-infection group (Z=-0.535,P=0.59 and Z=-0.027,P=0.98,respectively).However,mother's HBeAg positive status was closely related with neonatal HBeAg positive status (Pearson contingency coefficient was 0.928,P<0.01).The frequencies of mDC in newborns born to HBeAg positive mothers were significantly lower than those born to HBeAg negative mothers (0.60±0.57 vs 0.87±0.58; Z=-2.085,P<0.05).However,there was no significant quantitative differences of mDC and pDC between newborns born to HBV DNA positive mothers and born to negative mothers (Z=-1.272,P=0.20 and Z=-0.806,P=0.42,respectively).The frequencies of pDC were significantly lower in newborns born to mothers with HBV DNA> 1 × 107 copy/mL compared to newborns born to HBV DNA negative mothers (0.30±0.18 vs 0.64±0.55; t=-2.996,P=0.005).Conclusions HBeAg positive status of mothers may reduce neonatal frequencies of mDC.Neonatal frequencies

  13. HBsAg阴性献血者输血HBV感染残余风险分析%Residual Risk of Transfusion-transmitted HBV Infection in HBsAg-negative Blood Donors

    Institute of Scientific and Technical Information of China (English)

    方昌志; 傅颖媛; 钱榕; 熊丽红

    2012-01-01

    目的 分析HBsAg ELISA法检测阴性献血者的输血HBV感染残余风险,评价其感染状况.方法 采用瑞士罗氏诊断公司的Cobas s201核酸检测平台对2011年1月1日至12月31日57 141人份2遍ELISA检测阴性标本进行HBV DNA/HCV RNA/HIV-1,-2 RNA三项联合核酸检测(Cobas TaqScreen MPX 试剂),检测模式为混样检测+拆分检测,即先进行6标本混样检测,再对检测阳性标本进行拆分检测;对127人份拆分检测阳性标本进行分项鉴别试验及乙型肝炎两对半检测.结果 1)2遍ELISA法共检测标本60 037人份,检出HBsAg阴性标本57 141人份;2)对57 141人份阴性标本进行HBV DNA/HCV RNA/HIV-1,-2 RNA三项联检,共检出127人份病毒核酸阳性标本;3)127人份病毒核酸阳性标本进行分项鉴别试验,共检出HBV DNA 阳性标本69人份,输血HBV残余风险为0.12%,其定量检测结果以<20 U·mL-1为主(51人份,占73.9%);4)69人份HBV DNA阳性标本进行乙型肝炎两对半检测,发现乙型肝炎可疑窗口期感染6人份(占8.7%),隐匿性感染 52人份(占75.4%).结论 HBsAg ELISA法检测阴性献血者的输血HBV感染残余风险依然存在,其感染状况多以隐匿性感染为主,将核酸检测纳入血液筛查常规模式,可降低输血残余风险,提高输血安全.%Objective To analyze the residual risk of transfusion-transmitted HBV infection by ELISA method in HBsAg-negative blood donors,and to assess the infection status. Methods TaqScreen MPX test was performed for the detection of HBV DNA/HCV RNA/HIV-1,-2 RNA on a Cobas s201 system (Swiss Roche Diagnosis Company) in 57 141 HBsAg-negative blood donations. The detection was repeated two times. The detection mode was a combination of 6-sample minipool and individual positive donation test. Then, subentry identification and HBV marker detection were conducted in the 127 positive individual donations. Results A total of 60 037 samples were detected by twice ELISA test and 57 141 of them were

  14. Faster heme loss from hemoglobin E than HbS, in acidic pH: Effect of aminophospholipids

    Indian Academy of Sciences (India)

    Mousumi Banerjee; Malini Pramanik; Dipankar Bhattacharya; Mohini Lahiry; Samita Basu; Abhjit Chakrabarti

    2011-12-01

    We report studies on loss of heme at or below pH 3.0 from two clinically important hemoglobin variants, HbE and HbS, in the presence and absence of phopholipid membranes. The kinetics of heme loss has been studied at pH 3.0 to simulate the same at a faster rate than at physiological pH, for spectroscopic investigation. Results obtained from the study clearly establish the probable fate of the lost heme to partition into the phospholipid bilayer independent of the pH range. This is also of particular importance to membranes containing the aminophospholipid and cholesterol which are predominantly localized in the inner leaflet of erythrocytes. Absorption measurements indicated such loss of heme when the Soret peak at 415 nm blue-shifted to 380 nm at pH 3.0. The extent of this blue shift decreased from 35 nm to ∼15 nm in the presence of small unilammelar vesicles of both dimyristoyl- and dioleoyl-based phosphatidylcholine and phosphatidylethanolamine, indicating partitioning of the released heme in the membrane bilayer. The kinetics of heme loss was faster from HbE than HbA and HbS, obeying first-order reaction kinetics. Released heme could be involved in the premature destruction of erythrocytes in hemoglobin disorders.

  15. Application of HBS in multi-connected central air conditioning%HBS在多联中央空调中的应用

    Institute of Scientific and Technical Information of China (English)

    何惠湘

    2016-01-01

    HBS with its high reliability, easy wiring and other advantages is widely used in multi-connected central air conditioning products. In this paper, the HBS driver chip MM1192 and its communication circuit design and working process analysis are introduced in detail, and the application of the HBS protocol in the multi-connnected of the central air conditioning product controller is discussed.%HBS以其高可靠性、接线方便等优点被广泛应用在多联中央空调产品中。文章详细介绍了HBS驱动芯片MM1192及与之相连的通信电路的设计及工作过程分析,对多联中央空调产品控制器HBS协议的应用进行了探讨。

  16. SISTEMA INMUNE Y GENÉTICA:: UN ABORDAJE DIFERENTE A LA DIVERSIDAD DE ANTICUERPOS Immune System and Genetics:: A Different Approach to the Diversity of Antibodies

    Directory of Open Access Journals (Sweden)

    NUBIA ESTELA MATTA CAMACHO

    Full Text Available Es común encontrar en los libros de inmunología o de genética un capítulo con el título de -sistema inmune y genética-, sin embargo su asociación se centra en cómo la generación de anticuerpos rompió el paradigma -un gen, una proteína-, pues en el caso de la producción de anticuerpos, un gen produce millones de proteínas. El sistema inmune tiene muchos vínculos con la genética y la herencia; esta asociación se da porque cualquier sustancia o compuesto que produzca un organismo, es un antígeno potencial cuando es reconocido como extraño por el sistema inmune de otro organismo, sea este de la misma o de diferente especie. La producción de proteínas que potencialmente pueden ser antigénicas están ligadas al genotipo del individuo. La capacidad de responder y el tipo e intensidad de respuesta a antígenos también ha sido demostrado que están correlacionados con el genotipo del individuo en cuestión, así como deficiencias en las respuestas inmunes pueden estar asociadas con mutaciones o polimorfismos genéticos que resultan en la susceptibilidad a enfermedades infecciosas. Este manuscrito busca presentar ejemplos de la relación sistema inmune - genética, en la misma dirección de la conferencia ofrecida para la cátedra de Sede -Todo lo que usted quiere saber de genética y nunca se atrevió a preguntar-.It is common to find in immunology or genetic books a chapter entitled -immune system and genetics-; this association focuses on how the generation of antibodies broke the paradigm -one gene, one protein-, since in this case one gene generates millions of proteins. However, the immune system has many more links to genetics and heredity. For example, any substance or compound produced by an organism can be a potential antigen, when it is recognized as foreign by the immune system of another organism, belonging to the same or different species. The proteins, which are potentially antigenic are encoded by the individual s

  17. Seroprevalencia de anticuerpos bactericidas frente al meningococo C en Cantabria 10 meses tras la campaña de vacunación

    Directory of Open Access Journals (Sweden)

    Aledo Linos Alvaro Gonzalez de

    1998-01-01

    Full Text Available FUNDAMENTO: En febrero-marzo de 1997 se realizó en Cantabria (Comunidad Autónoma del Estado Español con 541.885 habitantes, de los cuales 107.787 tienen una edad entre 18 meses y 19 años una campaña de vacunación antimeningocócica dirigida a niños entre 18 meses y 19 años, que abarcó a todos sus municipios y alcanzó una cobertura mayor del 95%. En los 12 meses posteriores la eficacia vacunal fue del 95.68% en todas las edades. Para ayudar a decidir una revacunación se realizó un estudio de seroprevalencia de anticuerpos bactericidas en la población vacunada. MÉTODOS: en diciembre de 1997 se analizaron muestras de sangre de 414 niños vacunados, obtenidas al azar de extracciones oportunistas realizadas en Atención Primaria y en Hospitales Públicos de la Comunidad, y de niños escolarizados en las guarderías públicas dependientes de la Dirección General de Bienestar Social de Cantabria. Se analizó la tasa de anticuerpos bactericidas en el Centro Nacional de Microbiología, estableciéndose el nivel de "efecto vacunal" en la dilución 1/8. RESULTADOS: se obtuvieron los siguientes porcentajes de títulos > o = 1/8 (entre paréntesis, grupos de edad al vacunarse: 0% (18-24 meses, 4% (1.5 a 4 años, 7.1% (1.5 a 6 años, 51.3% (6 a 12 años y 81.2% (12 a 19 años. Debido a que la definición de "efecto vacunal" se cifró en la dilución 1/8 de forma artificial, y a que otros trabajos la cifran en 1/4, en 287 sueros con resultado <1/8 se repitió el ensayo bactericida con la dilución 1/4, resultando que 286 (el 99.6% también dieron negativo. Es decir, que el resultado final no variaría si situásemos el punto de corte en la dilución 1/4 en vez de en 1/8. No se encontraron diferencias significativas según la procedencia de municipios donde hubiera habido casos de meningitis C o no los hubiera habido. CONCLUSIONES: la actividad bactericida es muy baja en los menores de 4-6 años, inferior a lo publicado, y mayor por encima de esa

  18. Seroprevalencia de anticuerpos contra el poliovirus 1 en niños mexicanos Seroprevalence of antibodies against poliovirus type 1 in Mexican children

    Directory of Open Access Journals (Sweden)

    Juan Ruiz-Gómez

    2007-01-01

    Full Text Available OBJETIVO: Analizar la frecuencia y distribución de la prevalencia de anticuerpos contra el virus de la poliomielitis tipo 1 en niños menores de 10 años en México, además de contribuir a la evaluación del programa de vacunación. MATERIAL Y MÉTODOS: Se estudió la presencia de anticuerpos contra el poliovirus tipo 1 en una muestra de la Encuesta Nacional de Salud 2000. Los sueros se recolectaron entre noviembre de 1999 y junio de 2000 a nivel nacional. La muestra constó de 6 270 niños de uno a nueve años de edad y se utilizó la técnica de neutralización. RESULTADOS: La seropositividad fue de 99.3% (IC95%99.1-99.7. Se identificaron como factores de riesgo de susceptibilidad el analfabetismo (RM= 1.5, p= 0.002 y el bajo ingreso familiar (RM= 1.4, p= 0.0487 y como factor protector el acceso a la seguridad social (RM= 0.41, p=0.04. CONCLUSIONES: Las actividades del programa de vacunación que han llevado a cabo las instituciones de salud han dado resultados en el control y eliminación de la enfermedad. Sin embargo, los programas de vacunación no deben interrumpirse, incluso si se ha registrado 99.3% de seropositividad; no puede soslayarse que al convertir el 0.7% restante, se calcula que hay 190 000 niños susceptibles de contraer la enfermedad. Estos niños se localizan sobre todo en el sur del país.OBJECTIVE: To analyze the frequency and distribution of the prevalence of antibodies against the poliomyelitis type 1 virus in children 1-9 years old in Mexico. MATERIAL AND METHODS: Antibodies against poliovirus type 1 (neutralization method were studied in 6 270 sera selected from the 24 232 sera from children one to nine years old, collected by the 2000 National Health Survey (ENSA 2000 that was conducted from November 1999 to June 2000. RESULTS: Overall seroprevalence was 99.3% (95%CI: 99.1-99.7. Using bivariate analysis, absence of antibodies was shown to be associated with illiteracy (OR= 1.5, p=0.002 and low household income (OR= 1

  19. Asociación entre enfermedad cardiovascular y anticuerpos contra Chlamydia pneumoniae Association between cardiovascular disease and antibodies against Chlamydia pneumoniae

    Directory of Open Access Journals (Sweden)

    Guadalupe de los A García-Elorriaga

    2002-06-01

    Full Text Available Objetivo. Precisar si existe asociación entre enfermedad cardiovascular (ECV y anticuerpos contra Chlamydia en población mexicana. Material y métodos. Estudio transversal, realizado en la Unidad de Investigación en Inmunología e Infectología -Hospital de Infectología del Centro Médico Nacional La Raza (CMNR- y en el Servicio de Cirugía Cardiovascular y Asistencia Circulatoria, del Hospital General del CMNR, Instituto Mexicano del Seguro Social (IMSS, de agosto de 1998 a abril de 2000. Se determinaron anticuerpos IgG e IgM contra C. psittaci, C. trachomatis y C. pneumoniae mediante microinmunofluorescencia, en suero de 70 pacientes con ECV hospitalizados en el CMNR, mayores de 30 años, de uno u otro sexo, y se compararon con 140 sanos, pareados por edad y sexo. Se utilizaron muestras aleatorias simples, con un tamaño poblacional de 110, una prevalencia de 50% y un nivel de confianza de 99%. Para establecer la diferencia entre las proporciones de los títulos se utilizó ji cuadrada y se calculó la razón de momios. Resultados. El 94.3% (66/70 de los pacientes presentó IgG en contra de C. pneumoniae vs 37% (52/140 de los individuos sanos (pObjective. To evaluate the association between cardiovascular disease (CVD and antibodies against Chlamydia in Mexican population. Material and Methods. A cross-sectional study was conducted from August 1988 to April 2000, at the Immunology and Infectology Research Unit of Hospital de Infectología, Centro Médico Nacional La Raza (CMNR- and at the Cardiovascular Surgery and Circulatory Care, Hospital General CMNR, Instituto Mexicano del Seguro Social (IMSS. Study subjects were 70 CVD hospitalized patients, older than 30 years, from both genders. Serum IgG and IgM antibodies against C. psitaccii, C. trachomatis and C. pneumoniae were determined by microimmunofluorescence in study subjects and compared with those from 140 healthy individuals, matched by age and sex. Simple random sampling was used

  20. Prevalencia de anticuerpos contra virus dengue en el cantón de Golfito (2005 y en el Distrito Central de Puntarenas (2005-2006, Costa Rica

    Directory of Open Access Journals (Sweden)

    Margarita Lee-Lui

    2008-09-01

    Full Text Available Justificación y objetivo: La primo infección con el virus dengue varía desde asintomática hasta cuadros muy severos, como el dengue hemorrágico o el síndrome de choque por dengue. El Distrito Primero de Puntarenas ha sido una de las poblaciones más afectadas desde 1993, cuando aparecieron los primeros brotes, hasta hoy, con una disminución de la incidencia en los últimos años. La región Brunca fue la segunda en incidencia en el país en 1998, y si bien la endemicidad se ha mantenido, no existen estudios epidemiológicos sobre la prevalencia de esta enfermedad; los datos que aporta el Ministerio de Salud corresponden a la incidencia de casos clínicos en un período determinado. El objetivo de este estudio fue determinar la seroprevalencia en el Distrito Primero de Puntarenas y en Golfito para conocer la vulnerabilidad de la población de sufrir fiebre hemorrágica por dengue y analizar la situación asociada a los datos epidemiológicos de estas regiones, tales como edad, sexo y ubicación geográfica, ya que una población susceptible es la que presenta una alta seroprevalencia, unida a condiciones de hacinamiento y alta densidad vectorial. Métodos: El muestreo se realizó con el método EPI descrito por la OMS. Se recolectaron 210 muestras de suero en cada distrito, se desarrolló y estandarizó una prueba de ELISA de captura de antígeno para la detección de anticuerpos tipo IgG contra dengue, usando como referencia el método de reducción de placas de Dulbecco considerado estándar de oro. Resultados: La seroprevalencia contra dengue fue del 43.8% (92 y del 90% (189 en los distritos de Golfito y Central de Puntarenas, respectivamente, sin diferencias significativas respecto al sexo, la edad y la localidad. Se obtuvo un 83% y un 95% de correlación en Golfito y Puntarenas, respectivamente, entre los casos sintomáticos y la serología con el método estandarizado. Un 32% y un 42% del total de seropositivos en cada localidad

  1. Nivel de corte de los ELISAs para cuantificación de anticuerpos inducidos por la vacuna antimeningocócica VA-MENGOC-BC

    Directory of Open Access Journals (Sweden)

    Rolando Ochoa

    2001-06-01

    Full Text Available Para medir el grado de protección inducido por vacunas antimeningocócicas se ha establecido el Ensayo Bactericida en Suero (EBS y se perfeccionan otros ensayos inmunobiológicos, sin embargo, es necesario contar con pruebas sencillas como el ELISA, capaz de evaluar un gran número de muestras. Se estimó el nivel de corte de los ELISAs para la cuantificación de IgG humana contra los antígenos de VA-MENGOC-BC, vacuna antimeningocócica compuesta por vesículas proteicas de membrana externa de meningococo B y polisacárido capsular de meningococo C, con respecto a un panel de muestras de suero de lactantes, caracterizado por Ensayo Bactericida en Sangre Total (EBST. Los valores correspondientes a la máxima sensibilidad y especificidad fueron respectivamente; 2 μg/mL y 12 μg/mL para antipolisacárido C, y 1000 U/mL y 7000 U/mL para antiproteínas de membrana externa. La mayor coincidencia se obtuvo con 6 μg/mL y 2500 U/mL. Se evaluó otro panel de muestras de suero de adolescentes entre 14 y 18 años, por ELISA y EBS para Neisseria meningitidis serogrupos B y C, alcanzándose una buena concordancia. Doce años después de la inmunización con VA-MENGOC-BC persiste una importante concentración de anticuerpos contra los antígenos vacunales en los sueros estudiados.

  2. Prueba de Elisa indirecta para la detección de anticuerpos IgM para el diagnóstico de Leptospirosis humana

    Directory of Open Access Journals (Sweden)

    Manuel Céspedes Z

    2002-01-01

    Full Text Available Para el diagnóstico temprano de enfermedades con cuadro clínico inespecífico como la leptospirosis, es necesario la confirmación laboratorial mediante pruebas específicas, con la finalidad de que el diagnóstico sea más acertado y rápido. Objetivo: se realizó un estudio comparativo entre la prueba de microaglutinacion (MAT y la prueba de ELISA indirecta estandarizada con un "pool" de antígenos de Leptospira interrogans, para la detección de anticuerpos IgM, en muestras de suero de fase aguda de leptospirosis humana. Materiales y métodos: 40 muestras de pacientes con sospecha clínica y con títulos de 1:100-1:12800 por la prueba de MAT, 80 muestras negativas de pacientes aparentemente sanos con enfermedades como Brucelosis, Sífilis, Tifus murino, Hepatitis B, Fiebre Amarilla, Dengue y Enfermedad de Carrión fueron evaluados por ELISA IgM. Resultados: se obtuvo una sensibilidad de 97,5% y especificidad de 98,75%, no observándose reacción cruzada con otras enfermedades. Conclusión: ELISA IgM validado en el laboratorio es suficiente sensible, específico y de fácil aplicación para el uso como prueba de tamizaje en una infección por Leptospiras con la subsecuente confirmación por MAT.

  3. Generación de anticuerpos policlonales para la detección de la variante genotípica GIII de PVY, en cultivos de tomate de árbol y papa de Colombia

    Directory of Open Access Journals (Sweden)

    Yuliana Gallo García

    2012-04-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Título en ingles: Generation of policlonal antibodies for detection of the PVY genotypic variant GIII in tamarillo and potato crops from Colombia Resumen: El potyvirus PVY es uno de los agentes causales más frecuentemente asociados a problemas virales en cultivos de papa y tomate de árbol en Colombia. Dada la importancia económica de las enfermedades causadas por PVY y a la necesidad de generar material de siembra certificado por su sanidad viral, es fundamental la generación de herramientas de diagnóstico que permitan la detección temprana de este virus. En este trabajo se reporta la obtención de anticuerpos policlonales específicos, útiles para la detección del genotipo III de PVY (GIII, una de las tres variantes que recientemente han sido reportadas en cultivos de papa y tomate de árbol de la región Andina de Colombia. Como antígeno, se utilizó un péptido sintético diseñado a partir de la región variable del extremo N-terminal del gen de la cápside viral. La sensibilidad de los anticuerpos fue evaluada mediante pruebas de ELISA y dot-blot utilizando péptidos sintéticos. Se realizó una prueba piloto para validar el uso de los anticuerpos a partir de plantas sintomáticas y asintomáticas obtenidas de una región donde confluyen cultivos de ambas solanáceas, encontrándose que los anticuerpos generados ofrecen mayores niveles de detección que los anticuerpos comerciales comúnmente utilizados para detectar los serotipos PVY-O,C y PVY-N de este virus. Palabras clave: anticuerpos policlonales; ELISA; potyvirus; RT-PCR; virus Y de la papa Abstract: PVY is one of the potyvirus more frequently associated with viral infections in tomato and tamarillo crops in Colombia. Due to the economic impact of PVY and the need to certify seeds as virus-free it is important to develop diagnostic tools that allow its premature detection. In this work, the

  4. [Dynamic expression profile of HBsAg according to hepatic parenchyma cells' volume at different liver fibrosis stages in the immune clearance phase].

    Science.gov (United States)

    Wu, Zhe-bin; Cao, Hong; Liu, Ting; Wu, Ze-qian; Ke, Wei-min; Gao, Zhi-liang

    2012-10-01

    The aim of this study was to determine the dynamic expression profile of hepatitis B surface antigen (HBsAg) according to hepatic parenchyma cells' volume at different stages of liver fibrosis during the immune clearance phase. Eighty-nine patients with HBeAg-positive chronic hepatitis B (CHB) in the immune clearance stage were recruited for study. Each patient's serum HBsAg levels were detected by electrochemiluminescence. The serum HBsAg levels were apportioned according to hepatic parenchyma cells' volume at liver fibrosis stages 1, 2, 3, and 4 and compared by ANOVA. The unapportioned serum HBsAg levels (IU/mL) at liver fibrosis stages 1 (227.2+/-237.7), 2 (211.0+/-131.4), 3(300.1+/-144.6), and 4 (278.7+/-148.8) were not significantly different (all comparisons, P range: 0.061 to 0.759). However, when the serum HBsAg levels were apportioned by the same hepatic parenchyma cells' volume at liver fibrosis stages 1 (343.9+/-359.8), 2 (336.4+/-209.5), 3 (508.7+/-245.1), and 4 (525.2+/-274.8), the levels were significantly different (all comparisons, F = 3.045 and P = 0.033; stage 1 vs. 3, P = 0.041; stage 1 vs. 4, P = 0.046; stage 2 vs. 3, P = 0.028; stage 2 vs. 4, P = 0.034). During the immune clearance phase of chronic hepatitis B, increased HBsAg expression is associated with increased hepatic parenchyma cells' volume and progressive liver fibrosis stage.

  5. 化学发光免疫分析法检测低水平HBsAg与其他方法的比较%Chemiluminescence Immunoassay in Detection of Low Level HBsAg

    Institute of Scientific and Technical Information of China (English)

    李春莉; 杨桂林; 李秋红

    2014-01-01

    目的 比较并分析化学发光免疫分析法、ELISA法、胶体金法三种方法检测低水平HBsAg的结果.方法 以化学发光免疫分析仪Architect i2000检测结果作为参考,将100例<50 IU/ml标本分为3组,分别为:<1 IU/ml,1~5 IU/ml,>5 IU/ml.采用ELISA、胶体金法对3组标本进行检测,对测定结果进行比较分析.结果 应用化学发光免疫分析仪Ar-chitect i2000与ELISA测定低水平(<1 IU /ml) HBsAg的差异有统计学意义,在<1 IU /ml组,两者符合率仅为3.8%(P<0.05),(1~5) IU/ml组和>5 IU /ml组符合率分别为76.5%和100.0%.Architect i2000与胶体金法在测定低水平HB-sAg的差异有统计学意义(P<0.05),两种方法在<1 IU/ml组符合率为0,(1~5) IU /ml组符合率为2.9%,>5 IU /ml组符合率为100.0%.结论 化学发光免疫分析法检测低水平HBsAg (<5IU /ml)灵敏度明显高于ELISA和胶体金法.

  6. Péptidos y anticuerpos útiles para la elaboración de medicamentos para el tratamiento o prevención de enfermedades humanas neurodegenerativas, medicamentos así obtenidos y sus aplicaciones

    OpenAIRE

    Trullás Oliva, Ramón; Enguita, Marta; Abad, Alba

    2007-01-01

    Péptidos y anticuerpos útiles para la elaboración de medicamentos para el tratamiento o prevención de enfermedades humanas neurodegenerativas, medicamentos así obtenidos y sus aplicaciones. La presente invención describe inhibidores de la expresión génica del gen que codifica para la proteína NP1, que actúa como un inductor de apoptosis neural y neurodegeneración, útiles para la elaboración de medicamentos o composiciones farmacéuticas para el tratamiento o prevenci...

  7. Efficacy of lamivudine in prevention against virus reactivation during chemotherapy in HBsAg-positive patients with breast cancer%拉米夫定预防 HBsAg 阳性乳腺癌患者化学治疗中病毒再激活的疗效

    Institute of Scientific and Technical Information of China (English)

    刘宇; 李展翼; 黄泽楠; 黄勇

    2014-01-01

    Objective To observe the clinical efficacy of lamivudine in preventing the HBV reactiva-tion during chemotherapy in HBsAg-positive patients with breast cancer.Methods Forty-five subjects diag-nosed with HBsAg positive breast cancer,who did not receive anti-HBV treatment before,were enrolled in this study.All participants underwent 6 cycles of chemotherapy.Twenty-five patients in the treatment group were delivered with lamivudine at a dose of 0.1 mg/d prior to chemotherapy (starting from 1 week before and 1 2 weeks after chemotherapy).The other 20 cases who declined to receive lamivudine treatment were assigned into the control group (liver protection drugs were given based on the individual hepatic conditions).Following chemotherapy,the reactivation rate of HBV,liver damages,influence on chemotherapy,lamivudine-related ad-verse reactions and viral drug resistance and variability were compared between two groups.Results No pa-tients in the treatment group presented with HBV reactivation and the reactivation rate was 30% in the control group (P <0.05).The percentage of liver damages in the treatment group was 1 2%,lower compared with 45% in the control group (P <0.05).In the treatment group,the HBV reactivation had no influence upon chemotherapy.In the control group,one case (5%)discontinued the chemotherapy during the treatment,4 (20%)postponed the chemotherapy.No lamivudine-induced adverse reaction,HBV drug resistance or varia-bility was observed in the treatment group.Conclusion Preventive use of lamivudine can reduce the reactiva-tion rate of HBV during the chemotherapy in patients with breast cancer and yield no severe adverse reactions.%目的:观察接受化学治疗且 HBsAg 阳性的乳腺癌患者使用拉米夫定预防 HBV 再激活的疗效。方法既往均无接受抗 HBV 治疗的45例 HBsAg 阳性的乳腺癌患者被纳入研究,所有患者均接受6个疗程的化学治疗,其中于化学治疗前开始使用拉米夫定的25例被设为

  8. Comparison of Test Results of HBsAg in Serum and Plasma by Automatic ELISA Analysis%血清与血浆中HBsAg在全自动酶免分析检测结果比较

    Institute of Scientific and Technical Information of China (English)

    李小溪; 李筱涵; 郭晓东; 李波; 孙志强

    2011-01-01

    目的:探讨血浆取代血清检测乙肝标志物的检测结果以及临床意义.方法:选取我科检测乙肝标志物的血样30份,分别放在抗凝剂管和普通干燥试管,采用全自动酶免疫分析仪检测HBsAg,将检测结果进行OD及S/CO值统计处理,并进行比较分析.结果:血清与二种血浆检测结果相关系数均0.99,二种血浆与血清结果相关关系良好(P>0.0 5),无显著性差异.结论:血浆代替血清完全可以用于全自动酶标分析仪进行检测,既可以节省预处理时间,又可以减少标本在分离吸移血清过程中出现差错,值得临床推广和应用.%Objective: To investigate the clinical significance of the plasma instead of the serum in the detection ofHBV markers.Methods: A total of 30 blood samples were selected and detected HbsAg by automatic enzyme immunoassay analyzer, The test results were statistically treated and comparatively analyzed. Results: Serum and plasma results of two correlation coefficients were 0.99, two kinds of relationship between plasma and serum results well (P>0.05), no significant difference. Conclusion: Plasma instead of serum enzyme fully automated analyzer can be used to detect both pre-save time and reduce the serum specimens in the process of separation pipette errors and is worthy of promotion and application.

  9. 重组乙型肝炎疫苗和乙型肝炎表面抗原诱导细胞免疫应答%Cellular immune responses of recombinant hepatitis B(rHB)vaccine and HBsAg derived from Hansenular polymorpha cells

    Institute of Scientific and Technical Information of China (English)

    胡忠玉; 庄辉; 何鹏; 张瑞; 方鑫; 朱凤才; 邱少辉; 李河民; 汪华; 梁争论

    2008-01-01

    (ELISPOT)methods after stimulation in vitro with HBsAg MHC class Ⅰ peptide S28-39,respectively.At serial time points.the immunized mice were detected for IFN-γ by ELISPOT as above and for the lymphocytotoxicity test(CTL)by specific lysis assay.The levels of IFN-γ,IL-2,IL-5 and anti-HBs in mice induced by rHB vaccine were detected after single or three doses.Four adults were vaccinated with rHB vaccine according to 0,1 and 2 month schedule.The peripheral blood mononuclear cells(PBMCs)were collected at the 3,8,21,34 and 65 days after the first dose.The CD8+T cells with high purity obtained by sorting from PBMcs were stimulated with rHBsAg or HBsAg peptides.The SFC of IFN-γ,IL-2 and IL-4 of CD4+ and CD8+ T cells were determined by ELISPOT.Results The cytokine of IFN-γ became detectable on day 7 and its peak value appeared on day 14 by ELISPOT.The CTL was detected on day 7 and the maximum lysis of CTL appeared on day 28.The cellular immune response of IFN-γ of MNCs were significantly correlated with the doses vaccinated from 1 μg to 8 μg(Υpositive rates=0.951,Ppositive rates=0.049<0.05;rSFC=0.996,PSFC=0.000<0.05).IFN-γSFC of CD8+T cells were significantly associated with the doses from 1 μg to 4 μg(Υ=0.999,P=0.025<0.05).The HBsAg specific cellular immune and humoral responses of mice immunized with three doses were significantly higher than that with a single dose(P<0.05).The characteristics of IFN-γ,IL-2 and IL-4 of CD4+ and CD8+ T cells were variable between individuals immunized with the same rHB vaccine.The level of IL-2 and IL-4 of responders were significantly related to the titer of anti-HBs.Conclusion Data from this study showed the kinesis of cellular immunity in mice and adults vaccinated with rHBsAg or rHB vaccine respectively.and the characteristics of cellular immune response in adults induced by the vaccine.Our data provided the basis of standardizing the analysis of cellular immune response to rHB vaccine.

  10. Blood Staions’ Study on the Correlation between HBsAg detection via ELISA and the Reactive Samples with PCR%血站HBsAg反应性样本的ELISA与PCR法检测结果的相关性

    Institute of Scientific and Technical Information of China (English)

    冯娟

    2016-01-01

    Objective To explore the agreement between the testing results of reactive samples by ELISA for HBsAg and HBV DNA by PCR,so as that a more accurate testing may be provided for the donors.Methods PCR was apply to the reactive samples from volunteer donors who had been tested by ELISA for HBsAg to make a comparison between the results. Results By employing two ELISA reagents,698 samples are tested to be reacted positively in the total of122,675. Among the698 samples, there are382 have been equally detected positive by the two ELISA reagents.203 cases were found PCR positive, account for29.08%.179 cases are PCR negative, account for25.64%. Among the204 positive samples tested by one ELISA reagent,8 cases are found PCR positive, account for1.15%;196 negative cases existed, account for28.08%. By employing two ELISA reagents,5 samples were within the gray area. None of them were positive for PCR, account for0%; while5 negative were observed, account for0.72%; by employing one single ELISA reagent,107 cases were within the gray area and5 cases were PCR positive, comprise0.72%;102 are negative, account for14.61 %.Conclusion In the HBsAg blood screening, different ELISIA reagents showed different degrees of sensitivity and specificity. If double reagents are applied, the positive reaction results are more likely to be consistent with the results from HBV DNA. A few cases within gray area are also identical with them, but the development of HBV DNA should be closely observed and followed-up.%目的:探讨无偿献血者ELISA法检测HBsAg反应性样本与PCR检测HBV DNA结果的符合程度,为献血者提供更为准确的检测结果;并探讨新模式下,两种检测方法的结果报告及血源的召回制度。方法对无偿献血者ELISA法检测HBsAg反应性样本,采用PCR检测,并进行结果比较。结果122657份献血者样本中,2种ELISA试剂共检测出反应性样本698例,其中2种ELISA试剂检测均为阳性样本382

  11. Ultrasensitive potentiometric immunosensor based on SA and OCA techniques for immobilization of HBsAb with colloidal Au and polyvinyl butyral as matrixes.

    Science.gov (United States)

    Yuan, Ruo; Tang, Dianping; Chai, Yaqin; Zhong, Xia; Liu, Yan; Dai, Jianyuan

    2004-08-17

    A novel potentiometric immunosensor for detection of hepatitis B surface antigen (HBsAg) has been developed by means of self-assembly (SA) and opposite-charged adsorption (OCA) techniques to immobilize hepatitis B surface antibody (HBsAb) on a platinum electrode. A cleaned platinum electrode was first pretreated in the presence of 10% HNO3 and 2.5% K2CrO4 solution and held at -1.5 V (vs SCE) for 1 min to make it negatively charged and then immersed in a mixing solution containing hepatitis B surface antibody, colloidal gold (Au), and polyvinyl butyral (PVB). Finally, HBsAb was successfully immobilized onto the surface of the negatively charged platinum electrode modified nanosized gold and PVB sol-gel matrixes. The modified procedure was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The immobilized hepatitis B surface antibody exhibited direct electrochemical behavior toward hepatitis B surface antigen (HBsAg). The performance and factors influencing the performance of the resulting immunosensor were studied in detail. More than 95.7% of the results of the human serum samples obtained by this method were in agreement with those obtained by enzyme-linked immunosorbent assays (ELISAs). The resulting immunosensor exhibited fast potentiometric response (6 months).

  12. Recent structural studies on Dom34/aPelota and Hbs1/aEF1α: important factors for solving general problems of ribosomal stall in translation.

    Science.gov (United States)

    Kobayashi, Kan; Ishitani, Ryuichiro; Nureki, Osamu

    2013-01-01

    In the translation process, translating ribosomes usually move on an mRNA until they reach the stop codon. However, when ribosomes translate an aberrant mRNA, they stall. Then, ribosomes are rescued from the aberrant mRNA, and the aberrant mRNA is subsequently degraded. In eukaryotes, Pelota (Dom34 in yeast) and Hbs1 are responsible for solving general problems of ribosomal stall in translation. In archaea, aPelota and aEF1α, homologous to Pelota and Hbs1, respectively, are considered to be involved in that process. In recent years, great progress has been made in determining structures of Dom34/aPelota and Hbs1/aEF1α. In this review, we focus on the functional roles of Dom34/aPelota and Hbs1/aEF1α in ribosome rescue, based on recent structural studies of them. We will also present questions to be answered by future work.

  13. Identification of a natural intergenotypic recombinant hepatitis delta virus genotype 1 and 2 in Vietnamese HBsAg-positive patients.

    Science.gov (United States)

    Sy, B T; Nguyen, H M; Toan, N L; Song, L H; Tong, H V; Wolboldt, C; Binh, V Q; Kremsner, P G; Velavan, T P; Bock, C-T

    2015-01-01

    Hepatitis D virus (HDV) infection is acquired as a co- /superinfection of Hepatitis B virus (HBV) and can modulate the pathophysiology of chronic hepatitis B and related liver diseases including hepatocellular carcinoma. Among the eight distinct HDV genotypes reported, relatively few studies have attempted to investigate the prevalence of HDV mixed genotypes and RNA recombination of HDV. With a recorded prevalence of 10-20% HBV infection in Vietnam, this study investigated the HDV variability, HDV genotypes and HDV recombination among twenty-one HDV isolates in Vietnamese HBsAg-positive patients. HDV subgenomic and full-length genome sequences were obtained using newly established HDV-specific RT-PCR techniques. The nucleotide homology was observed from 74.6% to 99.4% among the investigated full-length genome of the HDV isolates. We observed HDV genotype 1 and HDV genotype 2 in the investigated Vietnamese patients. Although no HDV genotype mixtures were observed, we report here a newly identified recombinant of HDV genotypes (HDV 1 and HDV 2). The identified recombinant HDV isolate C03 revealed sequence homology to both HDV genotype 1 (nt1 to nt907) and HDV genotype 2 (nt908 to nt1675; HDAg coding region) with a breakpoint at nt908. Our findings demonstrate the prevalence of intergenotypic recombination between HDV genotypes 1 and 2 in a Vietnamese HBsAg-positive patient. Extended investigation on the distribution and prevalence of HDV, HDV mixed genotypes and recombinant HDV genotypes in a larger Vietnamese population offers vital insights into understanding of the micro-epidemiology of HDV and subsequent pathophysiology in chronic HBV- /HDV-related liver diseases.

  14. Estrés psicosocial, reactividad al estrés y marcadores bioquímicos LDL-Oxidada y anti-HSP60: Su relación en el ACV isquémico

    OpenAIRE

    Rodriguez de Cruz, Norys Margarita

    2016-01-01

    Tesis doctoral inédita, leída en la Universidad Autónoma de Madrid. Facultad de Medicina, Departamento de Psiquiatría. Fecha de lectura: 9 de febrero, 2016 A objeto de comparar y relacionar el estrés psicosocial, el índice de reactividad al estrés, la LDL oxidada y anticuerpos anti-HSP60 en individuos con enfermedad cerebrovascular isquémica (ACV) y sus respectivos controles (con similar edad y sexo), a 32 individuos de cada grupo les fue medido el estrés psicosocial por la esc...

  15. Prevalencia de anticuerpos anti-Chlamydia trachomatis y anti-Neisseria gonorrhoeae en grupos de individuos de la población mexicana Prevalence of antibodies against Chlamydia trachomatis and Neisseria gonorrhoeae in Mexican populations

    OpenAIRE

    María del Carmen Cravioto; Oscar Matamoros; Yvonne Villalobos-Zapata; Oscar Peña; Enrique García-Lara; Maribel Martínez; Julio Castelo; José Sifuentes-Osornio

    2003-01-01

    OBJETIVO: Estimar la prevalencia de infección por Chlamydia trachomatis (CT) y Neisseria gonorrhoeae (NG) en grupos de individuos con diferente riesgo para infecciones de transmisión sexual. MATERIAL Y MÉTODOS: Estudio transversal multicéntrico efectuado en el Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, de la Ciudad de México, de enero de 1992 a diciembre de 1993, en el que se estudiaron 945 individuos en edad reproductiva, 585 mujeres y 360 hombres. Según su riesgo p...

  16. Complicaciones clínicas en una serie de pacientes con anticuerpos anticardiolipina Clinical complications in a series of patients with anticardiolipin antibodies

    Directory of Open Access Journals (Sweden)

    A. Pujol

    2003-12-01

    Full Text Available Se han analizado las complicaciones clínicas determinadas con métodos objetivos en 68 pacientes de la Clínica Universitaria de Navarra con sospecha clínica de síndrome antifospolípido (SAF y presencia de anticuerpos anticardiolipina (ACA. Se consideraron para el estudio, pacientes con título de IgG superior a 23 GPL. La patología más prevalente fue la trombosis: trombosis venosas (42,6% y arteriales (22%. Otras complicaciones fueron abortos (23,8% de las 42 mujeres y trombocitopenia (12,1%. En cuanto a la localización de la trombosis venosa, el predominio correspondió a extremidades inferiores (51,7%, seguida de tromboflebitis superficial (27,5% y tromboembolismo pulmonar (20,6%. Entre las complicaciones arteriales, las más frecuentes fueron enfermedad cerebrovascular (86,6% y la cardiopatía isquémica (13,4%. No existió correlación entre la presencia de títulos elevados de ACA y la prevalencia o severidad de las manifestaciones clínicas. En la serie de 68 pacientes con ACA IgG 23 GPL se aprecia un elevado porcentaje de trombosis venosas y arteriales detectadas con métodos objetivos. La presencia de ACA constituye un factor de riesgo protrombótico.An analysis was made of clinical complications, determined with objective methods, in 68 patients at the University Clinic of Navarra with the clinical suspicion of antiphospholipid syndrome (APS and presence of anticardiolipin antibodies (ACA. Patients with IgG higher than 23 GPL were considered for the study. The most prevalent pathology was thrombosis: venous thrombosis (42.6% and arterial thrombosis (22%. Other complications were abortions (23.8% of the 42 women and thrombocytopenia (12.1%. With respect to the localisation of venous thrombosis, predominance corresponded to lower extremities (51.7%, followed by superficial thrombophlebitis (27.5% and pulmonary thromboembolism (20.6%. Among the arterial complications, the most frequent were cerebrovascular disease (86.6 % and

  17. Urticaria crónica con alteraciones de la función tiroidea y anticuerpos antiperoxidasa tiroidea Chronic urticaria with alterations of the thyroid function and thyroid peroxidase antibodies

    Directory of Open Access Journals (Sweden)

    Diego S. Fernández Romero

    2005-06-01

    Full Text Available La urticaria crónica es una enfermedad frecuente, caracterizada por la presencia de ronchas y/o angioedema con una duración superior a las 6 semanas. En un número importante de pacientes se comporta como una enfermedad autoinmune asociada frecuentemente con alteraciones en la función tiroidea y con la presencia de anticuerpos antitiroideos. Presentamos una serie de 70 pacientes consecutivos con diagnóstico de urticaria crónica a los cuales les investigamos la función tiroidea y la presencia de anticuerpos antiperoxidasa tiroidea. Siete (10% tenían diagnóstico de enfermedad tiroidea previa al momento de la consulta. A los 63 pacientes restantes se les estudió los niveles de tirotrofina sérica, 11 de los cuales (17% presentaron valores anormales, que sumados a los 7 con enfermedad previa llegan a 18 (26% con función tiroidea alterada. A 61 pacientes se les investigó anticuerpos antiperoxidasa tiroidea, 22 (36% fueron positivos. De 57 pacientes sin diagnóstico de patología tiroidea previa al momento de la consulta por urticaria, a los que se les estudió tanto los niveles de tirotrofina sérica como la presencia de anticuerpos antiperoxidasa tiroidea, 24 (42% presentaron alguno de los estudios alterados. El alto porcentaje de alteraciones tiroideas en nuestra serie de pacientes resalta la necesidad de estudiar la función tiroidea y la presencia de anticuerpos antiperoxidasa tiroidea en pacientes con urticaria crónica.Chronic urticaria is a frequent pathology, characterized by the presence of hives and/or angioedema lasting longer than 6 weeks. In an important number of patients it behaves as an autoimmune illness, frequently associated with alterations in thyroid function and thyroid antibodies. We herein describe a consecutive series of 70 patients with a diagnosis of chronic urticaria. Seven (10% had a diagnosis of thyroid illness previous to their first consultation. Thyroid function and thyroid antibodies were studied in the

  18. Detection of PMTV Using Polyclonal Antibodies Raised Against a Capsid-Specific Peptide Antigen / Detección de PMTV Utilizando Anticuerpos Policlonales Contra un Péptido Antigénico Derivado de la Cápside Viral

    Directory of Open Access Journals (Sweden)

    Yuliana Gallo García

    2013-12-01

    Full Text Available Potato mop-top virus (PMTV; genus Pomovirus;family Virgaviridae is the causing agent of the spraing disease in potato (Solanum tuberosum. PMTV is transmitted by Spongospora subterranea f. sp. subterranea (Sss. This disease has a widespread distribution in potato growing regions around the world. The possibility of obtaining strain specific antibodies at low cost can greatly increase the sensitivity and use of serological tests in seed certification programs, plant breeding and quarantine regulations to avoid dissemination of this injurious virus. This work presents an alternative procedure for the production of PMTV specific antibodies useful in serological test such as ELISAand lateral flow. In contrast to standard methods requiring theisolation of viral particles or expression of recombinant capsid, this method uses peptides mimicking the N-terminal region of PMTV capsid protein as antigen for the production of specific polyclonal antibodies. The antibodies were tested against bait plants grown in soil infested with viruliferous Sss, as well as potato plants obtained from naturally Sss infested fields in Colombia. PMTV was detected in 9/14 and 24/28 foliage samples of N. benthamiana and S. phureja, respectively. In the case of field plants, the virus wasdetected in eight out of 12 root tissues evaluated. The minimumpeptide concentration detected by ELISA was of the order of 0.1 nM. / Potato mop-top virus (PMTV; género Pomovirus; familia Virgaviridae es transmitido por Spongospora subterranea f. sp. subterranea (Sss, agente causal de la sarna polvosa de la papa. Esta enfermedad tiene una amplia distribución en las regiones cultivadoras de papa alrededor del mundo. La posibilidad de obtener anticuerpos específicos contra cepas de este virus, puede incrementar la sensibilidad y la utilización de pruebas serológicas en programas de certificación de semilla, mejoramiento genético y regulaciones cuarentenarias que eviten su diseminaci

  19. Detection of antibodies to hepatitis B core antigen using the Abbott ARCHITECT anti-HBc assay: analysis of borderline reactive sera.

    Science.gov (United States)

    Ollier, Laurence; Laffont, Catherine; Kechkekian, Aurore; Doglio, Alain; Giordanengo, Valérie

    2008-12-01

    Routine use of the automated chemiluminescent microparticle immunoassay Abbott ARCHITECT anti-HBc for diagnosis of hepatitis B is limited in case of borderline reactive sera with low signal close to the cut-off index. In order to determine the significance of anti-HBc detection when borderline reactivity occurs using the ARCHITECT anti-HBc assay, a comparative study was designed. 3540 serum samples collected over a 2-month period in the hospital of Nice were examined for markers of HBV infection (HBsAg, anti-HBs and anti-HBc). One hundred seven samples with sufficient volume and with borderline reactivity by the ARCHITECT assay were tested by two other anti-HBc assays, a microparticle enzyme immunoassay (MEIA, AxSYM Core, Abbott Laboratories, IL, USA) and an enzyme linked fluorescent assay (ELFA, VIDAS Anti-HBc Total II, bioMérieux, Lyon, France). Only 46 samples were confirmed by the AxSYM and the VIDAS assays. Additional serological information linked to patient history showed that the remaining samples (61) were false positives (11), had low titer of anti-HBc antibodies (13), or were inconclusive (37). This comparative study highlighted the existence of a grey zone around the cut-off index. Confirmative results through a different immunoassay are needed to confirm the diagnosis of HBV on borderline reactive sera using the ARCHITECT anti-HBc assay.

  20. Hepatitis D Virus Infection Among Hepatitis B Surface Antigen Carriers and in “Isolated anti-HBc” Antibodies Profile in Central Tunisia

    Science.gov (United States)

    Mhalla, Salma; Kadri, Yosr; Alibi, Sana; Letaief, Amel; Boukadida, Jalel; Hannachi, Naila

    2016-01-01

    Background: Hepatitis D Virus (HDV) causes accelerated liver diseases in patients with Hepatitis B Virus (HBV) infection. There is lack of data about its prevalence, related risk factors and interaction with HBV carriers in our country. Objectives: The aim of this study was to estimate the prevalence of hepatitis delta and associated risk factors among Hepatitis B surface antigen (HBsAg) and “isolated anti-HBc” profile carriers in central Tunisia. Patients and Methods: In this cross-sectional study, 540 patients with positive HBsAg and 109 “isolated anti-HBc” profile receiving care in a teaching hospital were tested for the presence of HDV serum-markers using commercially available enzyme immunoassay kit. HBV-DNA was detected by nested PCR in “isolated anti-HBc” profile group. Results: Prevalence of HDV was 8.1% in HBsAg carriers group, but it was significantly higher in active than inactive hepatitis (30.2% and 4.5%, respectively, OR = 9, 95% CI: [4.48-18.58]). There was no significant association between studied risk factors and HDV infection. In the “isolated anti-HBc” profile group, prevalence of HDV was 4.6% and HBV-DNA had negative result in all patients with positive results for HDV. Conclusions: Although HDV had low prevalence in our area, it is vital to plan preventive strategies for HDV spread as well as HBV prevention. It is particularly important to suspect HDV infection in active HBV carriers to manage a particularly severe dual infection. HDV infection should be suspected even in negative HBsAg patients having “isolated anti-HBc” profile. PMID:27110257

  1. HBV vaccination of HCV-infected patients with occult HBV infection and anti-HBc-positive blood donors

    Directory of Open Access Journals (Sweden)

    J.S.F. Pereira

    2006-04-01

    Full Text Available Anti-HBc positivity is a frequent cause of donation rejection at blood banks. Hepatitis B virus (HBV infection may also occur in HBsAg-negative patients, a situation denoted occult infection. Similarly, very low levels of HBV-DNA have also been found in the sera of patients with chronic hepatitis C virus (HCV infection, even in the absence of serum HBsAg. Initially we searched for HBV-DNA in serum of 100 blood donors and 50 HCV-infected patients who were HBsAg negative/anti-HBc positive by nested-PCR and by an HBV monitor commercial test for HBV-DNA. Anti-HBs seroconversion rates were measured in 100 blood donors and in 22 patients with chronic HCV infection after HBV vaccination to determine if the HBV vaccination could eliminate an occult HBV infection in these individuals. Occult HBV infection was detected in proportionally fewer blood donors (6/100 = 6% than chronic hepatitis C patients (12/50 = 24% (P 0.05. All subjects who were HBV-DNA(+ before the first dose of HBV vaccine (D1, became HBV-DNA(- after D1, D2, and D3. Among 22 HCV-positive patients, 10 HBV-DNA(+ and 12 HBV-DNA(-, seroconversion was observed in 9/10 (90% HBV-DNA(+ and in 9/12 (75% HBV-DNA(- subjects (P > 0.05. The disappearance of HBV-DNA in the majority of vaccinated patients suggests that residual HBV can be eliminated in patients with occult infection.

  2. HBV vaccination of HCV-infected patients with occult HBV infection and anti-HBc-positive blood donors.

    Science.gov (United States)

    Pereira, J S F; Gonçales, N S L; Silva, C; Lazarini, M S K; Pavan, M H P; Fais, V C; Gonçales Júnior, F L

    2006-04-01

    Anti-HBc positivity is a frequent cause of donation rejection at blood banks. Hepatitis B virus (HBV) infection may also occur in HBsAg-negative patients, a situation denoted occult infection. Similarly, very low levels of HBV-DNA have also been found in the sera of patients with chronic hepatitis C virus (HCV) infection, even in the absence of serum HBsAg. Initially we searched for HBV-DNA in serum of 100 blood donors and 50 HCV-infected patients who were HBsAg negative/anti-HBc positive by nested-PCR and by an HBV monitor commercial test for HBV-DNA. Anti-HBs seroconversion rates were measured in 100 blood donors and in 22 patients with chronic HCV infection after HBV vaccination to determine if the HBV vaccination could eliminate an occult HBV infection in these individuals. Occult HBV infection was detected in proportionally fewer blood donors (6/100 = 6%) than chronic hepatitis C patients (12/50 = 24%) (P 0.05). All subjects who were HBV-DNA(+) before the first dose of HBV vaccine (D1), became HBV-DNA(-) after D1, D2, and D3. Among 22 HCV-positive patients, 10 HBV-DNA(+) and 12 HBV-DNA(-), seroconversion was observed in 9/10 (90%) HBV-DNA(+) and in 9/12 (75%) HBV-DNA(-) subjects (P > 0.05). The disappearance of HBV-DNA in the majority of vaccinated patients suggests that residual HBV can be eliminated in patients with occult infection.

  3. Analysis of the feasibility of using a gold-immunochromatography assay to recheck HBsAg-positive samples%胶体金免疫层析法复检HBsAg阳性标本的可行性分析

    Institute of Scientific and Technical Information of China (English)

    夏伟伟; 姜国胜

    2016-01-01

    目的 评价利用胶体金免疫层析法(GICA)复检酶联免疫吸附试验(ELISA)所检测出的乙型肝炎表面抗原(H BsAg)阳性标本的效果.方法 分离用ELISA法筛选出的无黄疸、无溶血HBsAg阳性标本血清,按初检A值/cutoff值的大小分为A-E 5组,每组随机选取40例患者标本,另取40例健康者血清纳入对照组F组.每组标本分别用GICA法、ELISA法和化学发光免疫分析法(CLIA)进行复检.以CLIA法检测结果为金标准,计算并比较GICA法和ELISA法复检的假阳性率及假阴性率.结果 GICA法复检全部标本的假阳性率为0.当1≤A值/cutoff值<5时,GICA法的假阴性率为95.24%,同时,检测出1例因HBsAg滴度过高导致ELISA漏检的标本;当5≤A值/cutoff值<10时,GICA法的假阴性率降为23.68%;当A值/cutoff值≥10时,GICA法的假阴性率为0.结论 ELISA法HB-sAg初检A值/cutoff值≥10时,用GICA法复检为阳性,可及时发出报告;当A值/cutoff值<10时,为保证检验结果的准确性,除胶体金法外,还应使用ELISA等方法进行复检.

  4. 父母HBsAg携带状态对HBsAg阳性儿童心理行为的影响%The Influence of the State of Parents Carrying Hepatitis B Surface Antigens on the Mental Health and Behavior of Children with Hepatitis B Surface Antigens Masculine

    Institute of Scientific and Technical Information of China (English)

    李贤芬; 万国斌

    2009-01-01

    目的 探讨父母乙型肝炎表面抗原(HBsAg)携带状态对HBsAg阳性儿童心理行为的影响,为制定干预措施提供依据.方法 采用中国3~7岁儿童气质量表(Chinese preschool children temperament scale,CPTS)、中国8~12岁儿童气质量表(Chinese school children temperament scale,CSTS)和Achenhach儿童行为量表(Child Behavior Checklist,CBCL)以及自制的儿童基本情况调查表,对123例父母一方或双方为HBsAg阳性,75例父母HBsAg均为阴性的HBsAg阳性儿童进行测试和比较,并收集患儿个人及父母的健康状况等信息.结果 ①在低年龄组中实验蛆与对照组儿童在趣避性和心境2个气质维度上得分差异存在显著性(P<0.01),高年龄组两组儿童在节律性、趋避性和心境3个气质维度上得分差异存在显著性(P<0.05或P<0.01),②实验组儿童在退缩、焦虑/抑郁、社交同题以及行为问题总分等因子上得分明显高于对照组,差异具显著性(P<0.01或P<0.05).结论 父母为HBsAg携带者对HBsAg阳性儿童的心理行为产生不良影响,在治疗躯体疾病的同时,对HBsAg阳性儿童及家庭进行社会心理干预非常必要.

  5. Optimización del ensayo vibriocida colorimétrico para la determinación de anticuerpos séricos contra V. cholerae del serogrupo O139

    Directory of Open Access Journals (Sweden)

    Joivier Vichi-Lozada

    2011-01-01

    Full Text Available El título de anticuerpos vibriocidas es reconocido actualmente como el mejor marcador de inmunidad protectora ante la infección con Vibrio cholerae. Su evaluación permite estudiar la efectividad de vacunas durante ensayos preclínicos y clínicos, a través de la determinación de la funcionalidad de anticuerpos séricos, capaces de lisar células en presencia de complemento exógeno. Se han desarrollado distintos ensayos bactericidas con puntos finales y métodos de determinación del título vibriocida diferentes. La presencia de cápsula en el serogrupo O139 de Vibrio cholerae constituye un impedimento para la lisis eficiente de las células bajo condiciones del ensayo vibriocida utilizado en cepas del serogrupo O1. Por tal razón, se hace necesario adaptar los ensayos bactericidas existentes para V. cholerae O1, con la finalidad de utilizarlos con el serogrupo O139. A partir de un ensayo vibriocida colorimétrico, desarrollado para el serogrupo O1, se optimizaron las condiciones necesarias para una eficiente lisis celular del serogrupo O139 por anticuerpos específicos y el complemento. Este método colorimétrico tiene la ventaja que mediante la adición de glucosa y un indicador de pH al medio de cultivo utilizado para el crecimiento de las células sobrevivientes, puede determinarse visualmente el título de forma clara e inequívoca. Los mejores resultados se obtuvieron después del crecimiento de la cepa diana en cuñas de agar LB, al usar una concentración inicial de vibrión diana de 106 UFC/mL, una concentración inicial del complemento de 100 %, disolución salina fisiológica como diluente y tiempo de reacción de vibriolisis de una hora. Estas condiciones de experimentación permitieron obtener resultados exactos y precisos para V. cholerae O139.

  6. Séroprévalence de l’AgHBs chez la femme enceinte dans le centre du Maroc

    Science.gov (United States)

    Sbiti, Mohammed; Khalki, Hanane; Benbella, Imane; louzi, Lhoussaine

    2016-01-01

    La transmission materno-fœtale du virus de l'hépatite B (VHB) est un problème qui préoccupe toujours les autorités sanitaires à travers le monde et suscite la mise en place de mesures préventives renforcées. Le statut réplicatif du virus chez la mère séropositive, évalué par la positivité de l'AgHbe et le taux de la charge virale, conditionne le risque de contamination qui est plus important en période péri-natale. Les mères porteuses chroniques du VHB constituent un véritable réservoir de la transmission verticale de cette infection. Nous avons étudié, à travers cette enquête sur 1120 femmes enceintes Marocaines, la séroprévalence de l'AgHBs qui était de 2,35%, dans le but d'alimenter les données nationales sur ce sujet. Parmi ces femmes séropositives pour l'AgHBs, 79,1% avaient un AgHBe négatif et ont bénéficié d'une recherche moléculaire qui s'est révélée positive dans 89,4% des cas. La vaccination de la femme en âge de procréer est l'un des piliers majeurs de la prévention de la transmission verticale du VHB, seulement 2,4% de nos patientes étaient vaccinées. Ceci relève l'intérêt du dépistage au cours de la grossesse, qui doit se focaliser sur la sensibilisation à la vaccination de femmes séronégatives, et le suivi par biologie moléculaire des mères séropositives dans le but d'instaurer un traitement prophylactique adéquat. PMID:27795784

  7. Anti-Hepatitis B Virus X Protein in Sera Is One of the Markers of Development of Liver Cirrhosis and Liver Cancer Mediated by HBV

    Directory of Open Access Journals (Sweden)

    Hang Zhang

    2009-01-01

    Full Text Available Hepatitis B virus X protein (HBx plays a crucial role in the development of hepatocellular carcinoma (HCC. However, the significance of circulating antibody to hepatitis B virus X antigen (anti-HBx in sera remains unclear. In the present study, we examined the titers of anti-HBx (IgG in the sera from 173 patients with chronic hepatitis B (CHB, 106 liver cirrhosis (LC, and 61 HCC by enzyme-linked immunosorbent assay (ELISA, respectively. Our data showed that the positive rates of anti-HBx were higher in sera of LC (40.6% and HCC (34.4% than those of CHB (10.4%, P<.05. In all 40 patients with anti-HBx+ out of 340 patients, 39 (97.5% were HBsAg/HBeAg/anti-HBc+ and 1 (2.5% was anti-HBs+ (P<.01, suggesting that anti-HBx in sera is a marker of HBV replication rather than a protective antibody. Thus, our findings reveal that circulating anti-HBx in sera is one of the markers of development of LC and HCC mediated by HBV.

  8. Cloning, Eukaryotic Expression of Human ISG20 and Preliminary Study on the Effect of Its Anti-HBV

    Institute of Scientific and Technical Information of China (English)

    Youhua HAO; Dongliang YANG

    2008-01-01

    Human ISG20 gene was cloned and the effect of its anti-HBV was primarily studied. The ISG20 gene was amplified from HeLa cells by RT-PCR and recombinant vector expressing ISG20 was constructed by genetic engineering. The overexpression of ISG20 in HepG2 cells was detected by Western blot and the levels of secretion of HBs antigen and HBe antigen tested by ELISA. The results showed that: (1) Sequence of ISG20 cloned was consistent to that published in Genebank; (2) Recombinant vector expressing ISG20 could be expressed in HepG2 cells by transfection; (3) The overexpression of ISG20 protein could reduce the levels of the secretion of HBs antigen and HBe an-tigen in transfected HepG2 cells. It was suggested that the overexpression of recombinant ISG20 in culture cells could reduce the synthesis of HBV proteins.

  9. Simeprevir and Sofosbuvir Combination Treatment in a Patient with HCV Cirrhosis and HbS Beta 0-Thalassemia: Efficacy and Safety despite Baseline Hyperbilirubinemia

    OpenAIRE

    Nikolaos Papadopoulos; Melanie Deutsch; Athanasios Georgalas; Helias Poulakidas; Lazaros Karnesis

    2016-01-01

    Hyperbilirubinemia is an adverse reaction of simeprevir (SMV). The majority of these patients were taking concurrent ribavirin presenting elevated unconjugated hyperbilirubinemia due to hemolysis. However, cases of hepatic failure with elevated bilirubin level have also been reported in patients with decompensated cirrhosis. We describe a 51-year-old female patient with HbS beta 0-thalassemia and recently diagnosed compensated cirrhosis due to chronic hepatitis C infection. Laboratory evaluat...

  10. Existence of HbF Enhancer Haplotypes at HBS1L-MYB Intergenic Region in Transfusion-Dependent Saudi β-Thalassemia Patients

    Science.gov (United States)

    Vatte, Chittibabu; Borgio, J. Francis; Al-Rubaish, Abdullah; Nasserullah, Zaki A.; Jarrash, Sana Al; Sulaiman, Ahmed; Qutub, Hatem; Alsaleem, Hassan; Alzahrani, Alhusain J.; Steinberg, Martin H.

    2017-01-01

    Background and Objectives. β-Thalassemia and sickle cell disease are genetic disorders characterized by reduced and abnormal β-globin chain production, respectively. The elevation of fetal hemoglobin (HbF) can ameliorate the severity of these disorders. In sickle cell disease patients, the HbF level elevation is associated with three quantitative trait loci (QTLs), BCL11A, HBG2 promoter, and HBS1L-MYB intergenic region. This study elucidates the existence of the variants in these three QTLs to determine their association with HbF levels of transfusion-dependent Saudi β-thalassemia patients. Materials and Methods. A total of 174 transfusion-dependent β-thalassemia patients and 164 healthy controls from Eastern Province of Saudi Arabia were genotyped for fourteen single nucleotide polymorphisms (SNPs) from the three QTL regions using TaqMan assay on real-time PCR. Results. Genotype analysis revealed that six alleles of HBS1L-MYB QTL (rs9376090C p = 0.0009, rs9399137C p = 0.008, rs4895441G p = 0.004, rs9389269C p = 0.008, rs9402686A p = 0.008, and rs9494142C p = 0.002) were predominantly associated with β-thalassemia. In addition, haplotype analysis revealed that haplotypes of HBS1L-MYB (GCCGCAC p = 0.022) and HBG2 (GTT p = 0.009) were also predominantly associated with β-thalassemia. Furthermore, the HBS1L-MYB region also exhibited association with the high HbF cohort. Conclusion. The stimulation of HbF gene expression may provide alternative therapies for the amelioration of the disease severity of β-thalassemia. PMID:28280727

  11. Existence of HbF Enhancer Haplotypes at HBS1L-MYB Intergenic Region in Transfusion-Dependent Saudi β-Thalassemia Patients

    Directory of Open Access Journals (Sweden)

    Cyril Cyrus

    2017-01-01

    Full Text Available Background and Objectives. β-Thalassemia and sickle cell disease are genetic disorders characterized by reduced and abnormal β-globin chain production, respectively. The elevation of fetal hemoglobin (HbF can ameliorate the severity of these disorders. In sickle cell disease patients, the HbF level elevation is associated with three quantitative trait loci (QTLs, BCL11A, HBG2 promoter, and HBS1L-MYB intergenic region. This study elucidates the existence of the variants in these three QTLs to determine their association with HbF levels of transfusion-dependent Saudi β-thalassemia patients. Materials and Methods. A total of 174 transfusion-dependent β-thalassemia patients and 164 healthy controls from Eastern Province of Saudi Arabia were genotyped for fourteen single nucleotide polymorphisms (SNPs from the three QTL regions using TaqMan assay on real-time PCR. Results. Genotype analysis revealed that six alleles of HBS1L-MYB QTL (rs9376090C p=0.0009, rs9399137C p=0.008, rs4895441G p=0.004, rs9389269C p=0.008, rs9402686A p=0.008, and rs9494142C p=0.002 were predominantly associated with β-thalassemia. In addition, haplotype analysis revealed that haplotypes of HBS1L-MYB (GCCGCAC p=0.022 and HBG2 (GTT p=0.009 were also predominantly associated with β-thalassemia. Furthermore, the HBS1L-MYB region also exhibited association with the high HbF cohort. Conclusion. The stimulation of HbF gene expression may provide alternative therapies for the amelioration of the disease severity of β-thalassemia.

  12. Existence of HbF Enhancer Haplotypes at HBS1L-MYB Intergenic Region in Transfusion-Dependent Saudi β-Thalassemia Patients.

    Science.gov (United States)

    Cyrus, Cyril; Vatte, Chittibabu; Borgio, J Francis; Al-Rubaish, Abdullah; Chathoth, Shahanas; Nasserullah, Zaki A; Jarrash, Sana Al; Sulaiman, Ahmed; Qutub, Hatem; Alsaleem, Hassan; Alzahrani, Alhusain J; Steinberg, Martin H; Ali, Amein K Al

    2017-01-01

    Background and Objectives. β-Thalassemia and sickle cell disease are genetic disorders characterized by reduced and abnormal β-globin chain production, respectively. The elevation of fetal hemoglobin (HbF) can ameliorate the severity of these disorders. In sickle cell disease patients, the HbF level elevation is associated with three quantitative trait loci (QTLs), BCL11A, HBG2 promoter, and HBS1L-MYB intergenic region. This study elucidates the existence of the variants in these three QTLs to determine their association with HbF levels of transfusion-dependent Saudi β-thalassemia patients. Materials and Methods. A total of 174 transfusion-dependent β-thalassemia patients and 164 healthy controls from Eastern Province of Saudi Arabia were genotyped for fourteen single nucleotide polymorphisms (SNPs) from the three QTL regions using TaqMan assay on real-time PCR. Results. Genotype analysis revealed that six alleles of HBS1L-MYB QTL (rs9376090C p = 0.0009, rs9399137C p = 0.008, rs4895441G p = 0.004, rs9389269C p = 0.008, rs9402686A p = 0.008, and rs9494142C p = 0.002) were predominantly associated with β-thalassemia. In addition, haplotype analysis revealed that haplotypes of HBS1L-MYB (GCCGCAC p = 0.022) and HBG2 (GTT p = 0.009) were also predominantly associated with β-thalassemia. Furthermore, the HBS1L-MYB region also exhibited association with the high HbF cohort. Conclusion. The stimulation of HbF gene expression may provide alternative therapies for the amelioration of the disease severity of β-thalassemia.

  13. Diagnóstico rápido de citomegalovirus (CMV en pacientes inmunocomprometidos mediante anticuerpos monoclonales que reconocen proteinas precoces virales Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by using monoclonal antibodies against early viral antigens

    Directory of Open Access Journals (Sweden)

    Maritza Alvarez

    1989-06-01

    Full Text Available Se aplicó la técnica de detección de antigenos precoces fluorescentes (DAPF usando el anticuerpo monoclonal E-13 McAb, mediante el cual se lograron detectar 15 casos positivos a CMV de 75 muestras de orina o sangre ("buffy coat" tomadas de 52 pacientes inmunocomprometidos ingresados en el Instituto de Nefrología de ciudad Habana. Aplicando las técnicas clásicas de aislamiento en fibroblastos humanos diploides (MRC-5, se lograron aislar 12 cepas de CMV de casos previamente positivos por DAPF; lográndose además un aislamiento en una muestra reportada negativa por fluorescencia. Se observó una coincidencia de un 80% entre ambas técnicas. Se detectó la presencia de anticuerpos IgG contra CMV en todos los casos estudiados, utilizando para ello la técnica ELISA.A technique was applied to detect early fluorescent antigens (DEFA of cytomegalovirus (CMV using the E13 monoclonal antibodies in 52 immunocompromised patients hospitalized in the Nephrology Institute of Havana. Of the 75 urine or blood (buffy coat samples taken, 15 were found positive to CMV. Using classical diploide human fibroblast isolation technique, 12 CMV strains were isloation of previously detected positive samples by DEFA. In addition, CMV was isolated from one sample reported to be negative by DEFA. A coincidence of 80% was found between both techniques. With the ELISA test, all the sample studied have IgG antibodies to CMV.

  14. Influence of Paternal Serum HBV-DNA Load Levels and Pregnant Women's HBsAb on Paternal Vertical Transmission of Hepatitis B Virus%父亲血清HBV-DNA载量和孕母HBsAb对HBV父婴垂直传播的影响

    Institute of Scientific and Technical Information of China (English)

    陈顺萍; 张荣莲; 任坤海

    2014-01-01

    目的 探讨父亲血清HBV-DNA载量和孕母HBsAb对乙肝病毒(HBV)父婴垂直传播发生的影响,以期寻找阻断HBV父婴垂直传播的有效方法.方法 在患者知情同意的前提下,以HBsAg及HBV-DNA为指标筛检父亲HBsAg阳性、孕母HBVM全阴或者仅HBsAb阳性及HBV-DNA均为阴性的121个家庭作为研究对象,依据其新生儿脐带血HBV-DNA检测结果作为分组标准,将HBV-DNA检测阳性23例作为病例组,阴性98例作为对照组,进行病例对照研究.结果 ①新生儿脐带血HBV-DNA阳性率为19.0% (23/121);②父亲血清HBV-DNA载量与新生儿脐带血HBV-DNA阳性率存在剂量反应关系(趋势x2=60.108,P=0.000),受试者工作特征曲线(ROC曲线)分析表明:父亲血清HBV-DNA载量106拷贝/mL是预测HBV垂直传播发生较好的分界点;③孕母HBsAb阳性组与HBsAb阴性组其新生儿脐带血HBV-DNA阳性率差异有统计学意义(x2=12.399,P=0.000),当父亲血清HBV-DNA载量≥107拷贝/mL时,两组新生儿脐带血HBV-DNA阳性率差异无统计学意义(P>0.05).结论 父亲血清HBV-DNA阳性和孕母HBsAb阴性是HBV父婴垂直传播的危险因素,父亲血清HBV-DNA载量106拷贝/mL是较好的垂直传播发生的预测指标.

  15. Correlation analysis between HBsAg,HbeAg,HbeAb and HBcAb with HBV DNA in Lanzhou area and its clinical value%兰州地区 HBsAg、HBeAg、HBeAb、HBcAb 与 HBV DNA 的相关分析及临床价值

    Institute of Scientific and Technical Information of China (English)

    李彩东; 吴斌; 陈锡莲

    2014-01-01

    Objective To investigate the relationship between HBV DNA load with the serological markers(HB-M)HBsAg, HBeAg,HBeAb,HBcAb in the persons infected by chronic hepatitis B virus (HBV)in Lanzhou.Methods The real-time fluores-cent quantitative PCR was used to detect the HBV DNA load and the double antibody sandwich chemiluminesent immunoassay was used to measure the serum HBsAg,HbeAg,HbeAb and HbcAb levels in 724 cases of HBVinfection.Results The HBsAg level was positively correlated with the HBV DNA load in chronic HBV infection in Lanzhou area(r=0.342,P <0.05),there was an ob-vious positive correlation between HBeAg and HBV DNA load(r=0.463,P <0.05),the HBeAb level and HBV DNA load had the negative correlation (r=-0.227,P =0.001),the HBcAb level and HBV DNA load had no significantly correlation (r=-0.062, P =0.366).Conclusion There is obvious positive correlation between HBV DNA load with HBsAg,HBeAg in chronic HBV infec-tion in Lanzhou area,which indicating that the observation by combining HBsAg and HBeAg with HBV DNA can judge the infec-tious degree of the patients more accurately.%目的:探讨兰州地区慢性乙型肝炎病毒(HBV)感染者血清乙型肝炎病毒脱氧核糖核酸(HBV DNA)载量与乙型肝炎病毒血清学标志物(HBV-M)HBsAg、HBeAg、HBeAb、HBcAb 的相关性。方法724例 HBV 感染者分别利用实时荧光定量PCR 法检测 HBV DNA 载量,运用双抗体夹心化学发光免疫分析法检测血清 HBsAg、HBeAg、HBeAb、HBcAb 水平。结果兰州地区慢性 HBV 感染者 HBsAg 水平与 HBV DNA 载量呈正相关关系(r=0.342,P <0.05),HBeAg 水平与 HBV DNA 载量呈正相关关系(r=0.463,P <0.05),HBeAb 水平与 HBV DNA 载量之间呈负相关关系(r =-0.227,P =0.001),HBcAb 水平与HBV DNA 载量变化无相关性(r=-0.062,P =0.366)。结论兰州地区慢性 HBV 感染者 HBV DNA 载量和 HBsAg、HBeAg均有明显的正相关

  16. Investigation of occult hepatitis B virus infection in anti-hbc positive patients from a liver clinic.

    Directory of Open Access Journals (Sweden)

    Maria Carmela Martinez

    Full Text Available Occult hepatitis B infection (OBI is manifested by presence of very low levels (<200IU/mL of Hepatitis B viral DNA (HBV DNA in the blood and the liver while exhibiting undetectable HBV surface antigen (HBsAg. The molecular mechanisms underlying this occurrence are still not completely understood. This study investigated the prevalence of OBI in a high-risk Australian population and compared the HBV S gene sequences of our cohort with reference sequences. Serum from HBV DNA positive, HBsAg negative, and hepatitis B core antibody (anti-HBc positive patients (study cohort were obtained from samples tested at SEALS Serology Laboratory using the Abbott Architect, as part of screening and diagnostic testing. From a total of 228,108 samples reviewed, 1,451 patients were tested for all three OBI markers. Only 10 patients (0.69% out of the 1,451 patients were found to fit the selection criteria for OBI. Sequence analysis of the HBV S gene from 5 suspected OBI infected patients showed increased sequence variability in the 'a' epitope of the major hydrophilic region compared to reference sequences. In addition, a total of eight consistent nucleotide substitutions resulting in seven amino acid changes were observed, and three patients had truncated S gene sequence. These mutations appeared to be stable and may result in alterations in HBsAg conformation. These may negatively impact the affinity of hepatitis B surface antibody (anti-HBs and may explain the false negative results in serological HBV diagnosis. These changes may also enable the virus to persist in the liver by evading immune surveillance. Further studies on a bigger cohort are required to determine whether these amino acid variations have been acquired in the process of immune escape and serve as markers of OBI.

  17. Evaluación en animales del efecto protector de una inmunoglobulina anti Pseudomonas aeruginosa para uso terapéutico