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Sample records for anticuerpos anti hbs

  1. Anticuerpo anti-Dectin-1 humano, hibridoma productor de dicho anticuerpo y sus aplicaciones

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    Llorente Gómez, María de las Mercedes; Fernández Ruíz, Elena; Galán Díez, Marta; Kremer, Leonor; Casasnovas, José María

    2008-01-01

    Anticuerpo anti-Dectin-1 humano, hibridoma productor de dicho anticuerpo y sus aplicaciones. La presente invención consiste en el hibridoma MGD3 y el anticuerpo monocional producido por él (denominado también MGD3), que reconoce de forma especifica al receptor de membrana Decti-1 humano. El anticuerpo MGD3 es capaz de inhibir la unión de Dectin-1 a su ligando natural, los -glucanos que son componentes de la pared de los hongos. Además, bloquea específicamente la un...

  2. Identified OAS3 gene variants associated with coexistence of HBsAg and anti-HBs in chronic HBV infection.

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    Wang, Sa; Wang, Jing; Fan, Mengjie; Li, Tengyan; Pan, Hong; Wang, Xi; Liu, Hankui; Lin, Qiongfen; Zhang, Jianguo; Guan, Liping; Zhernakova, Daria V; O'Brien, Stephen J; Feng, Zhenru; Chang, Le; Dai, Erhei; Lu, Jianhua; Xi, Hongli; Zeng, Zheng; Yu, Yanyan; Wang, Binbin

    2018-03-27

    The underlying mechanism of coexistence of hepatitis B surface antigen (HBsAg) and hepatitis B surface antigen antibody (anti-HBs) is still controversial. To identify the host genetic factors related to this unusual clinical phenomenon, a two-staged study was conducted in the Chinese Han population. In the first stage, we performed a case-control (1:1) age, gender matched study of 101 cases with concurrent HBsAg and anti-HBs and 102 controls with negative HBsAg and positive anti-HBs using whole exome sequencing. In the second validation stage, we directly sequence the 16 exomes on the OAS3 gene in two dependent cohorts of 48 cases and 200 controls. Although in the first stage, a genome-wide association study of 58,563 polymorphism variants in 101 cases and 102 controls found no significant loci (P-value ≤ 0.05/58563), and neither locus achieved a conservative genome-wide significance threshold (P-value ≤ 5e-08), gene burden analysis showed that OAS3 gene rare variants were associated with the coexistence of HBsAg and anti-HBs. (P-value =4.127e-06 ≤ 0.05/6994). 16 rare variants were screened out from 21 cases and 3 controls. In the second validation stage, one case with a new different rare variant was identified. Fisher's exact test of all 149 cases and 302 controls showed that the rare coding-sequence mutations were more frequent in cases versus controls [P-value=7.299e-09, OR=17.27, 95% CI (5.01-58.72)]. Protein-coding rare variations on the OAS3 gene are associated with the coexistence of HBsAg and anti-HBs in patients with chronic HBV infection in Chinese Han population. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  3. Anti-HBc testing for blood donations in areas with intermediate hepatitis B endemicity Detección de anticuerpos anti-HBc en sangre donada en lugares donde la hepatitis B tiene endemicidad intermedia

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    Celina Maria Turchi Martelli

    1999-07-01

    Full Text Available En otros estudios se ha evaluado el menor riesgo de infecciones transmitidas por transfusión sanguínea que acarrea el tamizaje de anticuerpos contra los antígenos nucleares de la hepatitis B (anti-HBc, sumado a la prueba detectora de antígenos de superficie (HbsAg. No obstante, la mayoría de estos estudios se basaron en datos procedentes de zonas con baja endemicidad por hepatitis B o de bancos de sangre de países desarrollados, donde la mayoría de la sangre proviene de personas que han donado previamente. A fin de examinar los pros y los contras del tamizaje de anticuerpos anti-HBc en el Brasil, los autores describen los antecedentes y la interpretación de las pruebas de tamizaje para prevenir la hepatitis postransfusión. También presentan los datos sobre el tamizaje de la hepatitis B en todas las regiones del Brasil. No hay pruebas suficientes para suspender el tamizaje de los anticuerpos anti-HBc en el país. Debe darse alta prioridad a comparar el costo-efectividad de un marcador basado en la biología molecular con el de la actual prueba detectora de anticuerpos anti-HBc aplicada comúnmente, a fin de mejorar las medidas de tamizaje que garantizan la inocuidad de la sangre.

  4. Predicting response to HBV vaccination in people with positive anti-HBc but negative HBsAg and anti-HBs.

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    Kabir, Ali; Keshvari, Maryam; Kashani, Amir Hossein Faghihi; Alavian, Seyed-Moayed

    2008-01-01

    There are 5.1-6.5% of people with positive anti-HBc in Iran. The aim of this study was to assess the predicting factors of response to hepatitis B vaccination in anti-HBc positive subjects. Total response rate to vaccination was 79.8% (75 cases) and 67.9% (38 cases) in cases and controls, respectively. Nineteen persons (20.2%) in cases and 18 persons (32.1%) of controls had negative anti-HBs even after three doses of HB vaccination. Factor associated with decreased response to vaccination was prior history of being HBsAg positive (OR = 1.3, p = 0.01). In a quasi-experimental study, 94 people with negative HBsAg, negative anti-HBs and positive anti-HBc (cases) and 56 persons with negative HBsAg, anti-HBs and anti-HBc (controls) were vaccinated at zero, one and six months with recombinant hepatitis B vaccine. Successful immunization was defined by anti-HBs antibody titer > or =10 mIU/mL. The rate of response to hepatitis B vaccination is nearly like other studies but somewhat different. Higher percent of married cases together with higher percent of positive HBsAg in spouses may explain the slight difference in the response to vaccination in cases in comparison with controls as a result of booster like effect that seldom happens because of recurrent contacts between the subjects and the HBsAg positive spouses spontaneously.

  5. Epidemiological characteristics of the carriers with coexistence of HBsAg and anti-HBs based on a community cohort study.

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    Pu, Z; Li, D; Wang, A; Su, H; Shao, Z; Zhang, J; Ji, Z; Gao, J; Choi, B C K; Yan, Y

    2016-04-01

    The coexistence of HBsAg and anti-HBs is an atypical serological pattern in HBV infection. There is no epidemiological characteristics of this serological pattern in the community and there is controversy over the molecular mechanisms underlying this pattern. We investigated the epidemiological characteristics of the carriers with HBsAg and anti-HBs in a longitudinal community cohort study. The prevalence of this atypical serological pattern was 2.93% (122/4169) in HBsAg-positive populations. The prevalence progressively increased with age from 40 to 70 years old. The rate of HBeAg positive and detectable HBV DNA were both significantly higher in carriers with this pattern than in carriers who were HBsAg positive but anti-HBs negative (26/122 verse 598/4047, P = 0.046; 86/122 verse 275/529,P coexistence HBsAg and anti-HBs, 14.81% of the carriers lost their anti-HBs. Viral sequencing showed that carriers with coexistence of HBsAg and anti-HBs had higher numbers of residue changes within the S gene than carriers who were HBsAg positive but anti-HBs negative (2.42 verse 1.33 changes per 100 residues, P coexistence of HBsAg and anti-HBs is a unique serological pattern which may be associated with an increased risk of adverse clinical outcome and may be related to HBsAg immune variants which have genotypic heterogeneity. © 2015 John Wiley & Sons Ltd.

  6. Evolution of full-length genomes of HBV quasispecies in sera of patients with a coexistence of HBsAg and anti-HBs antibodies.

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    Zhou, Tai-Cheng; Li, Xiao; Li, Long; Li, Xiao-Fei; Zhang, Liang; Wei, Jia

    2017-04-06

    Although the evolutionary changes of viral quasispecies are correlated to the pathological status of a disease, little is known in the coexistence of hepatitis B surface antigen (HBsAg) and antibodies to these antigens (anti-HBs). To examine evolutionary changes in hepatitis B virus (HBV) and their relationship to the coexistence of HBsAg and anti-HBs antibodies, HBV genomes in patients with a coexistence of HBsAg and anti-HBs antibodies (experimental group) and HBsAg positive without anti-HBs (control group) were assessed. Our results showed that quasispecies diversity was significantly higher in the experimental group for large HBsAg (LHBsAg), middle HBsAg (MHBsAg), and HBsAg genes. LHBsAg harbored dN/dS values eight times higher in the experimental group; however, the mean dN/dS ratios in genes HbxAg, Pol and PreC/C of the experimental patients had an opposite trend. Phylogenetic trees in the experimental group were more complex than the control group. More positive selection sites, mutations and deletions were observed in the experimental group in specific regions. Furthermore, several amino acid variants in epitopes were potentially associated with the immune evasion. In conclusion, cumulative evolutionary changes in HBV genome that facilitate immune evasion provide insights into the genetic mechanism of a coexistence of HBsAg and anti-HBs antibodies.

  7. Mutation in the S gene a determinant of the hepatitis B virus associated with concomitant HBsAg and anti-HBs in a population in Northeastern Brazil.

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    de Campos Albuquerque, Ingrid; Sousa, Marinilde Teles; Santos, Max Diego Cruz; Nunes, Jomar Diogo Costa; Moraes, Maria Josélia Diniz; Gomes-Gouvêa, Michele Soares; Pinho, João Renato Rebelo; Carrilho, Flair José; Fonseca, Lena Maria Barros; de Sousa Paiva Ferreira, Adalgisa

    2017-03-01

    Mutations in the a determinant of S gene may develop co-existence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) in the serum of infected hepatitis B virus (HBV) individuals. Mutations in this region may change the antigenicity of HBsAg, which in turn, lead to escape of neutralizing action of anti-HBs antibodies. This study identified individuals with concomitant HBsAg and anti-HBs serological markers in individuals of Maranhão, Northeastern Brazil. Samples from a population-based study were evaluated for HBsAg, anti-HBs, and anti-HBc, and those that tested positive for simultaneous HBsAg and anti-HBs were submitted to HBV DNA quantification and S gene characterization by Sanger sequencing. Mutations were investigated in the a determinant located in major hydrophilic region (MHR) of the S gene. Among 3,984 samples analyzed, 92 (2.3%) were positive for HBsAg and three had the atypical HBsAg and anti-HBs-positive profile (3.26%). The frequency of HBsAg and anti-HBs co-existence was similar to previous studies. Only one individual harbored mutation in the S gene a determinant associated with this profile. Little is known about this phenomenon; however, studies as ours may contribute for future enlightenment of this important issue. J. Med. Virol. 89:458-462, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  8. Occult hepatitis B virus infection in anti-HBs-positive infants born to HBsAg-positive mothers in China.

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    Haixia Su

    Full Text Available OBJECTIVE: To investigate the prevalence of occult HBV infection (OBI among children and to characterize virology of occult HBV, we conducted an epidemiological survey. METHODS: 186 HB-vaccinated infants born to HBsAg-positive mothers were included in the study. Serological tests for HBV markers were performed using commercial ELISA kits. Real-time quantitative PCR and nested PCR were used to detect HBV DNA. PCR products of the C and pre-S/S regions were sequenced and analyzed. RESULTS: 1.61% (3/186 infants were HBsAg positive, and 4.92% (9/183 infants were considered as occult infection. The viral load of mothers was associated with occult infection (P = 0.020. Incomplete three-dose injections of HB vaccine was associated with HBV infection (P = 0.022. Six OBI infants were positive for anti-HBs, but their titers were not greater than 100 mIU/mL. Seven isolated HBV pre-S/S sequences were obtained from nine OBI infants. Three of the sequences were genotype C, and four of the sequences were genotype C/D. Escape mutation S143L was found in the four sequences of genotype C/D. All seven sequences lacked G145R and other escape mutation in S region. CONCLUSIONS: Occult HBV infection was detected in anti-HBs positive infants born to HBsAg-positive mothers in China. Occult infection was associated with absent anti-HBs or with low anti-HBs level, high maternal viral loads and escape mutations in the S gene.

  9. The effects of booster vaccination on hepatitis B vaccine in anti-HBs negative infants of HBsAg-positive mothers after primary vaccination.

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    Gu, Hua; Yao, Jun; Zhu, Wei; Lv, Huakun; Cheng, Suyun; Ling, Luoya; Xia, Shichang; Chen, Yongdi

    2013-06-01

    The purpose of this study was to investigate the changes in anti-HBs IgG levels after booster vaccinations in anti-HBs negative infants of HBsAg-positive mothers. After primary vaccination, the immunization effects of different dosages of booster vaccinations of hepatitis B vaccine (CHO) were compared. A group of 472 newborns were vaccinated with three-dose hepatitis B vaccine at birth, 1 mo and 6 mo of age. Blood serum was collected within 6-12 mo after the third dose, and HBsAg, anti-HBs and anti-HBc levels were determined. Of this group, 101 infants who were both anti-HBs and HBsAg negative were revaccinated with 20 μg hepatitis B vaccine (CHO), and their antibody titers were monitored. Among these 101 infants, the anti- HBs positive rates (defined as anti-HBs ≥ 100 mIU/ml) differed after the first and the third dose (79% and 90%, respectively (panti-HBs GMTs after booster vaccination were 10-fold larger than those before booster vaccination. We conclude that a single booster dose is generally adequate for infants of HBsAg-positive mothers, whereas a further booster dose should be given for non-responders.

  10. Predictive factors for anti-HBs status after 1 booster dose of hepatitis B vaccine.

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    Lu, I-Cheng; Jean, Mei-Chu Yen; Lin, Chi-Wei; Chen, Wei-Hung; Perng, Daw-Shyong; Lin, Chih-Wen; Chuang, Hung-Yi

    2016-09-01

    In Taiwan, infants need to receive 3 doses of hepatitis B virus (HBV) vaccine under the public health policy from the government. However, there are many young adults who even though received complete HBV vaccination in their childhood would lose the positive response of anti-hepatitis B surface antibody (HBs) and need the booster dose of HBV vaccine. The aim of our study is to determine the powerful predictive factor for screening the candidates who need only 1 booster dose of HB vaccine then they can regain positive postbooster anti-HBs status (≧10 mIU/mL) or protective postbooster anti-HBs status (≧100 mIU/mL).We recruited 103 university freshmen who were born after July 1986 with complete HBV vaccination in childhood, but displayed negative results for hepatitis B surface antigen and anti-HBs levels at their health examinations upon university entry. They received 1 booster dose of HB vaccine, and their anti-HBs titers were rechecked 4 weeks after the booster administration. Multivariate analysis logistic regression for positive postbooster anti-HBs status (≧10 mIU/mL, model 1) and protective postbooster anti-HBs status (≧100 mIU/mL, model 2) was done with predictive factors of prebooster anti-HBs level, body mass index, serum glutamate pyruvate transaminase level, and sex.Twenty-four students got positive postbooster anti-HBs status (10-100 mIU/mL) and 50 students got protective postbooster anti-HBs status (≧100 mIU/mL). In the model of multivariate analysis logistic regression for positive postbooster anti-HBs status (≧10 mIU/mL), prebooster anti-HBs level was the strongest predictive factor. The odds ratio was 218.645 and the P value was 0.001. Even in the model of multivariate analysis logistic regression for protective postbooster anti-HBs status (≧100 mIU/mL), prebooster anti-HBs level was still the strongest predictive factor, but the odds ratio of a protective booster effect was 2.143, with 95% confidence interval between 1

  11. Development of anti-hepatitis B surface (HBs) antibodies after HBs antigen loss in HIV-hepatitis B virus co-infected patients.

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    Boyd, Anders; Canini, Laetitia; Gozlan, Joël; Lascoux-Combe, Caroline; Miailhes, Patrick; Fonquernie, Laurent; Girard, Pierre-Marie; Lacombe, Karine

    2017-10-01

    Hepatitis B surface antigen (HBsAg)-seroconversion, or loss of HBsAg and acquisition of anti-hepatitis B surface (HBs) antibodies, defines functional cure of chronic hepatitis B virus (HBV) infection. After HBsAg-loss, little is known regarding the development of anti-HBs antibodies and even less so in individuals co-infected with HIV. To determine anti-HBs antibody kinetics after HBsAg-loss and explore determinants of HBsAg-seroconversion in HIV-HBV co-infected patients. Patients enrolled in the French HIV-HBV cohort were included if they had >1 study visit after HBsAg-loss. Individual patient kinetics of anti-HBs antibody levels were modeled over time using mixed-effect non-linear regression, whereby maximum specific growth rate and maximal level of antibody production were estimated from a Gompertz growth equation. Fourteen (4.6%) of 308 co-infected patients followed in the cohort exhibited HBsAg-loss, all of whom were undergoing antiretroviral therapy. Nine (64.3%) of these patients achieved HBsAg-seroconversion during a median 3.0 years (IQR=1.1-5.1) after HBsAg-loss. Across individuals with HBsAg-seroconversion, the fastest rates of antibody growth ranged between 0.57-1.93year -1 (population maximum growth rate=1.02) and antibody production plateaued between 2.09-3.66 log 10 mIU/mL at the end of follow-up (population maximal antibody levels=2.66). Patients with HBsAg-seroconversion had substantial decreases in HBV DNA viral loads (P=0.03) and proportion with elevated ALT levels (P=0.02) and HBeAg-positive serology (P=0.08). No such differences were observed in those without HBsAg-seroconversion. Most co-infected patients with HBsAg-seroconversion produced and maintained stable antibody levels, yet kinetics of anti-HBs production were much slower compared to those observed post-vaccination or after clearance of acute HBV-infection. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. GAMBARAN ANTI HBc POSITIF PADA DONOR DARAH DENGAN HbsAG NEGATIF

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    Susila Sastri

    2009-09-01

    Full Text Available AbstrakPemeriksaan HBsAg saja untuk skrining hepatitis B (HBV belum dapat menjamin donor darah bebas dari HBV sehingga darah donor belum memenuhi persyaratan untuk ditransfusikan. Darah donor yang akan ditranfusikan hendaklah memenuhi syarat diantaranya donor tidak pernah menderita HBV. Skrining darah donor terhadap HBV pada PMI hanya dengan uji HBsAg saja dimana HBsAg akan negatif pada stadium HBV tertentu, pada hal donor menderita atau dalam masa penyembuhan HBV. Anti-HBc dapat memberi informasi tentang perjalanan HBV bila digabungkan dengan marker HBV lain dan anti-HBc bertahan lebih lama dalam darah dibandingkan dengan marker lain. Donor darah HBsAg negatif dengan anti-HBc positif dari beberapa penelitian terdahulu masih ada yang mengandung HBV-DNA dan dapat menularkan HBV.Telah dilakukan penelitian terhadap donor darah dengan melakukan uji anti-HBc secara deskriptif terhadap donor darah dengan HBsAg negatif (n=100 pada UTD PMI Cabang Padang secara cross sectional study. Sampel diambil secara proportional random sampling. Darah sampel adalah darah HBsAg negatif dengan VDRL, HVC, HIV  negatif , semuanya diperiksa dengan dengan ELISATujuan penelitian untuk melihat gambaran anti-HBc positif pada donor darah dengan HBsAg negatif dan melihat hubungan antara indeks HBsAg dengan indeks anti-HBc. Pemeriksaan anti-HBc dilakukan dengan ELISA, alat dan reagen keluaran yang sama.Hasil penelitian menunjukkan frekuensi anti-HBc positif pada donor darah dengan HBsAg negatif sebanyak 27%, terutama pada laki-laki berumur antara 20-29 tahun (44,4%. Terdapat korelasi positif antara indeks HBsAg negatif dengan indeks anti-HBc (r = 0,02. Anti-HBc positif banyak ditemukan pada indeks HBsAg negatif 0,21-0,60 (76%.Kesimpulan, darah donor dengan HBsAg negatif yang selama ini dianggap aman untuk transfusi terbukti masih mungkin menularkan HBV dengan ditemukannya anti-HBc yang positif. Karena itu perlu pemeriksaan lanjutan DNA HBV pada donor darah dengan Hbs

  13. Experimental HBsAg/anti-HBs complex assay for prediction of HBeAg loss in chronic hepatitis B patients treated with pegylated interferon and adefovir

    NARCIS (Netherlands)

    de Niet, Annikki; Jansen, Louis; Zaaijer, Hans L.; Klause, Ursula; Takkenberg, Bart; Janssen, Harry L. A.; Chu, Tom; Petric, Rosemary; Reesink, Hendrik W.

    2014-01-01

    Background: We studied whether hepatitis B surface antigen (HBsAg)/anti-HBs immune complex levels in chronic hepatitis B (CHB) patients receiving antiviral therapy could be used as a response marker at baseline (BL) or early during treatment to predict treatment outcome. Methods: An experimental

  14. Mutations in the S gene and in the overlapping reverse transcriptase region in chronic hepatitis B Chinese patients with coexistence of HBsAg and anti-HBs

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    Feng Ding

    2016-01-01

    Conclusions: In chronic patients, the concurrent HBsAg/anti-HBs serological profile is associated with an increased aa variability in several key areas of HBV genome. Additional research on these genetic mutants are needed to clarify their biological significance for viral persistence.

  15. Clinical patterns associated with the concurrent detection of anti-HBs and HBV DNA.

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    Anastasiou, Olympia E; Widera, Marek; Korth, Johannes; Kefalakes, Helenie; Katsounas, Antonios; Hilgard, Gudrun; Gerken, Guido; Canbay, Ali; Ciesek, Sandra; Verheyen, Jens

    2018-02-01

    Simultaneous detection of anti-HBs and HBV DNA is a rare serological combination and has been described in acute and chronic HBV infection. To scrutinize viral and clinical patterns associated with concurrent detection of anti-HBs and HBV DNA. Simultaneous detection of anti-HBs and HBV DNA was observed in 64/1444 (4.4%) patients treated for HBV infection at the University Hospital of Essen from 2006 to 2016 (8 with acute, 20 with reactivated, and 36 chronic HBV infection). Clinical data and laboratory parameters were analyzed. Regions of the small hepatitis B surface antigen (SHB) and the reverse transcriptase (RT) were sequenced using next generation sequencing (NGS). Among the 64 patients with detectable HBV DNA and anti-HBs, 17 were HBsAg negative (HBsAg[-]), and two had acute liver failure. Patients with acute HBV infection had fewer genotype specific amino acid substitutions in the SHB region than patients with reactivated HBV infection (4 [4.5] vs 9 [16.25], P = 0.043). However, we could observe a significantly higher number of mutations in the a-determinant region when comparing chronically infected patients to patients with acute infection (0 [1] vs 1 [1], P = 0.044). The ratio of nonsynonymous to synonymous mutations (Ka/Ks) was on average >1 for the SHB region and 1) in the SHB region indicates that anti-HBs might have exerted selection pressure on the HBsAg. In three cases the diagnosis of acute HBV infection would have been at least delayed by only focusing on HBsAg testing. © 2017 Wiley Periodicals, Inc.

  16. Experiencia clínica con el uso de los anticuerpos anti-CdtB y anti-vinculina en pacientes con diarrea en México

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    Schmulson, M.; Balbuena, R.; Corona de Law, C.

    2016-01-01

    Introducción: Se ha validado el uso de los anticuerpos anti-CdtB/anti-vinculina como biomarcadores para discriminar el SII-D de la EII, más no hay experiencia con ellos en Latinoamérica. Materiales y métodos: Se analizaron pacientes que consultaron a una clínica de TGIF/motilidad en los últimos 7 meses por diarrea con dolor/distensión abdominal, y a quienes se les solicitó estos anticuerpos. Los pacientes fueron diagnosticados mediante los criterios de Roma III o con trastornos orgánicos. ...

  17. Anticuerpos anti-Ro y anti-La. Caracterización molecular, correlación clínica e inmunogenética

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    Sergio Guevara Pacheco

    1992-06-01

    Full Text Available Los anticuerpos anti-Ro y anti-La se vienen estudiando desde hace 25 años, pero su estructura molecular, asociación clínica y relación genética adquieren relevancia en los últimos siete (7 años. Estos anticuerpos tienen especial lugar en la nosología del LES y del síndrome Sjögren. En el lupus neonatal los anticuerpos tienen un papel patológico directo, mientras que en otras condiciones (vasculitis, nefritis , el daño tisular puede resultar por depósito de complejos inmunes en las estructuras vasculares.

  18. Prevalencia de anticuerpos anti envoltura nuclear y sus isotipos en sueros positivos para anticuerpos antinucleares Prevalence of antinuclear envelope antibodies and their isotypes in sera positive for antinuclear antibodies

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    Miriam Arcavi

    2006-08-01

    Full Text Available Los anticuerpos antinucleares detectados por inmunofluorescencia indirecta en células HEp-2 presentan una gran variedad de imágenes, entre ellas el patrón de envoltura nuclear que suele ser un hallazgo poco frecuente. Se procesaron 2594 sueros en los cuales se detectó un 37.6% de anticuerpos antinucleares. La prevalencia de anticuerpos anti-envoltura nuclear (ANEA fue del 1.2% presentando una alta asociación con hepatopatías autoinmunes (83% y baja con lupus eritematoso sistémico. En los 21 sueros de los pacientes que presentaron ANEA no se detectaron anticuerpos anti-ADNn hallándose 28.6% de anticuerpos anti-músculo liso y 19% de anticuerpos anti-mitocondriales. El corte triple de tejido de rata mostró ser un sustrato menos sensible que HEp-2 para la detección de ANEA. Al utilizar conjugados dirigidos contra diferentes isotipos de anticuerpos para la detección de ANEA, se encontró: 90.5% de IgG, 66.6% de IgA y 9.5% de IgM. Dos de los pacientes presentaron ANEA-IgA a altos títulos (³1:160 en ausencia de ANEA-IgG. En este trabajo se destaca la importancia de realizar pruebas complementarias que detecten anticuerpos anti-músculo liso, anti-mitocondriales y anti-ADNn, para orientar el diagnóstico clínico de los pacientes que presentan ANEA. Además, sostiene la postura de utilizar como conjugado para IFI-HEp2 anticuerpos anti-inmunoglobulinas totales en lugar de anti-IgG hasta tanto se clarifique el rol que juegan los anticuerpos IgA en estas enfermedades autoimunes.Antinuclear antibodies detected in HEp-2 cells by indirect immunofluorescence assay display a great variety of images, including the nuclear envelope pattern. This is quite a less frequent finding. Two thousand five hundred and ninety-four sera were processed, and 37.6% of ANA were detected. The prevalence of anti-nuclear envelope antibodies (ANEA was of 1.2%, with a high association with autoimmune liver diseases (83% and a low association with systemic lupus

  19. Acute hepatitis B virus infection with simultaneous high HBsAg and high anti-HBs signals in a previously HBV vaccinated HIV-1 positive patient.

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    van Dommelen, Laura; Verbon, Annelies; van Doorn, H Rogier; Goossens, Valère J

    2010-03-01

    We present a case of a clinical manifest hepatitis B virus infection and a potentially misleading HBV serological profile in an HIV-1 positive patient despite previous HBV vaccination. The patient presented with an acute hepatitis B and there was no indication of chronic HBV infection or the presence of a mutation in the 'a' determinant. Remarkably, simultaneously with high HBV surface antigen and HBV viral load, high anti-HBs antibodies were present. If, due to previous HBV vaccination only anti-HBs was tested in this patient, the result of the high anti-HBs antibodies could be very misleading and offering a false sense of security. Our findings contribute to the ongoing discussion on how to assess HBV specific immunological memory and determining the role of HBV booster vaccinations in immunocompromised individuals.

  20. Effects of hepatitis B vaccine boosters on anti-HBs-negative children after primary immunization.

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    Lu, Shunshun; Ren, Jingjing; Li, Qian; Jiang, Zhenggang; Chen, Yongdi; Xu, Kaijin; Ruan, Bing; Yang, Shigui; Xie, Tiansheng; Yang, Linna; Li, Jing; Yao, Jun

    2017-04-03

    This study was aimed at evaluating the changes of hepatitis B surface antibody (anti-HBs) titer after booster vaccinations in 5-15-year-old children with negative antibodies (vaccination during infancy. The participants were divided into 3 groups according to their pre-booster anti-HBs level: Group I, vaccination (0-1-6 month, 20 µg), and changes in the levels of antibodies were examined at 4 time-points (one month after the first and the third dose, one year and 5 years after the third dose). The seroprotective rate (defined as anti-HBs ≥10.0 mIU/mL) among 225 subjects at the 4 time-points were 93.8%, 100%, 83.6% and 73.4%, respectively (χ 2 = 90.29, p anti-HBs geometric mean titer (GMT) in Group III were always higher than those in the other 2 groups (all p effect of a 3 -dose booster revaccination is good, and the booster-induced immune response was correlated with the pre-booster titer level, and ≥1.0 mIU/mL ensuring a robust positive response, whereas titers below this value may indicate the need for a course of booster vaccination.

  1. Detección de anticuerpos anti-Leishmania (Trypanosomatidae en poblaciones caninas del departamento de Sucre, Colombia

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    Luís Enrique Paternina Tuirán

    2016-01-01

    Full Text Available El objetivo de la investigación fue determinar la prevalencia de anticuerpos anti-Leishmania en perros del departamento de Sucre, Colombia. Se analizaron 122 perros del área rural de los municipios de Ovejas, Sampués y Sincelejo, mediante la técnica de inmunofluorescencia indirecta (IFI. Anticuerpos anti-Leishmania fueron detectados en el 69,6 % (IC95 % = 61,1 – 78,2 de la población canina estudiada. En el municipio de Sincelejo la seropositividad fue del 72 % (IC95 % = 61,1 – 82,8, en el municipio de Sampués del 68,4 % (IC95 % = 52,3 – 84,5 y en el municipio de Ovejas del 55,5 % (IC95 % = 21,2 – 86,3. Los animales seropositivos fueron principalmente caninos jóvenes y adultos. El 17,6 % de los perros seropositivos mostró signos clínicos compatibles con leishmaniasis canina, entre los cuales la onicogrifosis, alopecia y caquexia fueron los más comunes. La alta frecuencia de caninos con anticuerpos a Leishmania detectada en el presente estudio, pone de manifiesto la hiperendemicidad de la leishmaniasis canina en esta zona del país, así como el alto riesgo de brotes epidémicos de la enfermedad.

  2. Hepatitis B reactivation characterized by HBsAg negativity and anti-HbsAg antibodies persistence in haematopoietic stem cell transplanted patient after lamivudine withdrawal.

    Science.gov (United States)

    Cerva, C; Maffongelli, G; Svicher, V; Salpini, R; Colagrossi, L; Battisti, A; Mariotti, B; Cerretti, R; Cudillo, L; Sarmati, L

    2017-08-15

    HBV reactivation is associated with high mortality rates in hematopoietic stem cell transplantation (HSCT) and prophylactic lamivudine (LMV) treatment is suggested to prevent this phenomenon. However, the duration of LMV treatment in HSCT patients is not fully defined and the time of immune recovery is considered the best parameter for a drug to be safely interrupted. In patients undergoing allogeneic HSCT, the time of immune recovery is not easy to define and may take years after transplantation and prolonged LMV treatments, which can lead to drug-resistant viral strains. An anti-HBc-positive hematological patient who was undergoing prolonged immunosuppression and who experienced HBV reactivation 3 months after the suspension of a prolonged LMV prophylaxis is described. HBV-DNA matching an atypical serological profile characterized by HbsAg negativity and anti-HBs positivity was detected in the patient. The genotypic analysis of the HBV strain identified T127P, F170FL and S204R mutations of HbsAg, which can hinder HBsAg recognition in a diagnostic assay. HBV reactivation in the HSCT host can be sustained by HBsAg viral variants with characteristics of altered immunogenicity that cannot be detected by usual laboratory tests. This clinical case description suggests the importance of screening for serum HBV-DNA levels in the diagnosis of HBV reactivation and monitoring HBV-DNA after prophylaxis suspension, particularly in HSCT subjects who have undergone prolonged periods of LMV treatment.

  3. [Vaccination against hepatitis B on the Ivory Coast: study of the anti-HBs response in healthy adult subjects carrying only anti-HBc antibodies before vaccination].

    Science.gov (United States)

    Ouattara, S A; Meite, M; Aron, Y

    1986-01-01

    A total of 103 volunteers, from 18 to 55 years of age, have received the hevac B Pasteur 5 micrograms vaccine subcutane on sly; one injection a month during three months and one booster injection after one year. The study of the anti-HBs reaction of the subjects, with regard to the serological status to the hepatite B virus before inoculation, has shown that only 78.8% of the subjects, who are only positive towards the anti-HBc antibody, will develop an anti-HBs response of primary type with a relatively low value. In contrast, all anti-HBc and/or anti-HBs subjects, who are positive before inoculation, react with relatively high anti-HBs values right after the first injection, of the seronegative subjects before inoculation. 93.3% will develop an anti-HBs seroconversion after the complete inoculation procedure.

  4. [Requirement of standardizing anti-HBs assay methods in Japan for HBV infection-preventing strategy--discrepancy of anti-HBs measurements among three different kits widely used in Japan].

    Science.gov (United States)

    Ogata, Norio

    2006-09-01

    The strategy to eliminate hepatitis B virus (HBV) infection by administrating an HB vaccine is changing worldwide; however, this is not the case in Japan. An important concern about the HBV infection-preventing strategy in Japan may be that the assay methods for the antibody to hepatitis B surface antigen (anti-HBs) are not standardized. The minimum protective anti-HBs titer against HBV infection has been established as 10 mIU/ml by World Health Organization (WHO) -standardized assay methods worldwide, but that is still determined as a "positive" test result by the passive hemagglutination (PHA) method in Japan. We compared anti-HBs measurements in given samples among PHA(Mycell II, Institute of Immunology), chemiluminescent enzyme immunoassay (CLEIA) (Lumipulse, Fujirebio), and chemiluminescent immunoassay (CLIA) (Architect, Abbott), all of which are currently in wide use in Japan. First, anti-HBs measurements in serum from individuals who received a yeast-derived recombinant HB vaccine composed of the major surface protein of either subtype adr or subtype ayw were compared. The results clearly showed that in subtype adr-vaccinees CLIA underestimated the anti-HBs amount compared with CLEIA and PHA, but in ayw-vaccinees, the discordance in the measurements among the three kits was not prominent. Second, anti-HBs measurements in standard or calibration solutions of each assay kit were compared. Surprisingly, CLEIA showed higher measurements in all three kit-associated standard or calibration solutions than CLIA. Thus, the anti-HBs titer of 10 mIU/ml is difficult to introduce in Japan as the minimum protective level against HBV infection. Efforts to standardize anti-HBs assay methods are expected to share international evidence about the HBV infection-preventing strategy.

  5. Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix

    Directory of Open Access Journals (Sweden)

    Vázquez-Corzo Sara

    2003-01-01

    Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.

  6. Reactivation of viral replication in anti-HBe positive chronic HBsAg carriers

    DEFF Research Database (Denmark)

    Krogsgaard, K; Aldershvile, J; Kryger, Peter

    1990-01-01

    Reactivation of hepatitis B virus replication was investigated in an unselected group of 44 HBV DNA negative, anti-HBe positive chronic HBsAg carriers. Twenty-five patients (54%) were intravenous drug addicts and 7 (16%) were male homosexuals. Sixteen patients had evidence of delta infection...... and five of the seven male homosexuals had human immunodeficiency virus infection. The patients were followed for 1 to 180 months (median, 24 months) while HBV DNA negative, anti-HBe positive. Reactivation, defined as reappearance of HBV DNA or HBeAg, or both, was detected in six patients corresponding...... to an annual reactivation rate of 5%. Reactivation in four patients was detected by reversion to HBV DNA positivity only, whereas HBeAg/anti-HBe status remained unchanged. Two patients became both HBV DNA and HBeAg positive. None of the patients developed hepatitis-like symptoms and transaminase elevation...

  7. Antioxidative Flavonol Glucuronides and Anti-HBsAg Flavonol from Rotala rotundifolia

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    Li-Jie Zhang

    2011-10-01

    Full Text Available Two new flavonol glucuronides, quercetin 3-O-β-d-2″-acetylglucuronide (1 and quercetin 3-O-β-d-2″-acetylglucuronide methyl ester (2, along with four known flavonoids (3-6 were isolated from the whole parts of Rotala rotundifolia. The structures of 1 and 2 were elucidated by application of various spectroscopic methods, including 1D and 2D NMR techniques. Biological evaluation showed that all of isolated flavonoid compounds have potent anti-oxidative activities by DPPH and superoxide anion methods, and kaempferol (3 and quercetin (4 exhibited significant anti-HBV activity assayed by anti-HBsAg production. The HPLC fingerprint with photodiode array detection (HPLC-DAD for quality control of R. rotundifolia partitioned EtOAc layer was also established.

  8. Valor pronóstico de los anticuerpos anti-Chlamydia pneumoniae en la cardiopatía isquémica

    OpenAIRE

    González Castañeda, C.; Pérez Castrillón, José Luis; Casero Lambas, A.; Mazón Ramos, M.Á.; Cañibano González, M. Á.; López Bello, M.; Herreros Fernández, V.

    2004-01-01

    Producción Científica Se ha relacionado la enfermedad arterial coronaria con infección por Chlamydia pneumoniae, pero existen pocos estudios que valoren la presencia de anticuerpos frente a este microorganismo y el pronóstico de los pacientes con cardiopatía isquémica. El objetivo de nuestro estudio fue valorar el impacto de la positividad de anticuerpos anti-Chlamydia pneumoniae en la morbimortalidad asociada a la cardiopatía isquémica.

  9. Frecuencia de anticuerpos anti Trypanozoma cruzi en pacientes portadores de marcapasos de la Clinica San Pedro Claver de Bogotá

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    Guillermo Mora

    2007-12-01

    Conclusiones. La prevalencia de anticuerpos anti-T. cruzi en pacientes portadores de marcapaso es alta. La implantación de marcapasos se presentó más tempranamente en el grupo seropositivo. No hay adecuada sospecha clínica ni información al paciente sobre el diagnóstico de la enfermedad de Chagas.

  10. Evaluation of HBsAg and anti-HBc assays in saliva and dried blood spot samples according HIV status.

    Science.gov (United States)

    Flores, Geane Lopes; Cruz, Helena Medina; Potsch, Denise Vigo; May, Silvia Beatriz; Brandão-Mello, Carlos Eduardo; Pires, Marcia Maria Amendola; Pilotto, Jose Henrique; Lewis-Ximenez, Lia Laura; Lampe, Elisabeth; Villar, Livia Melo

    2017-09-01

    Influence of HIV status in HBV markers detection in saliva and dried blood spots (DBS) was not well established. This study aims to evaluate the performance of optimized commercial immunoassay for identifying HBsAg and anti-HBc in saliva and DBS according HIV status. A sum of 535 individuals grouped as HIV + , HBV + , HIV/HBV + and HIV/HBV- were recruited where 347 and 188 were included for HBsAg and anti-HBc evaluation, respectively. Serum, DBS collected in Whatman 903 paper and saliva obtained using salivette device were analyzed using EIA. Increased sample volume and ROC curve analysis for cut off determination were used for DBS and saliva testing. HBsAg detection in saliva and DBS exhibited sensitivities of 80.9% and 85.6% and specificities of 86.8% and 96.3%. Sensitivity of anti-HBc in saliva and DBS were 82.4% and 76.9% and specificities in saliva and DBS were 96.9% and 91.7%. Low sensitivities were observed for HBsAg (62%) and anti-HBc (47%) detection in saliva of HIV/HBV+ individuals. OD values were also lower for HBsAg detection in DBS and saliva of HIV/HBV+ individuals compared to their serum samples. Statistical significance was found for sensitivities in HBsAg detection between saliva and DBS demonstrating high sensitivity for DBS specimens. In conclusion, HIV status or antiretroviral treatment appears to interfere in the performance of HBsAg and anti-HBc detection in DBS and saliva samples using the adapted commercial EIA. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Anti-HBs in immunized children with cuban hepatitis B vaccine and impact of booster dose after five years

    International Nuclear Information System (INIS)

    Dahifar, H.; Mousavi, F.; Ghorbani, A.

    2008-01-01

    Hepatitis B virus infection and associated diseases are a major public health problem. This study was planned to find out the persistence of antibody against hepatitis B surface antigen in Iranian vaccinated children after five years. Anti-HBs titers in a group of healthy good - responder children who were vaccinated with Cuban hepatitis B vaccine in infancy were measured after five years. Children with antibody titers <100 mlU/ml were revaccinated and retested after four weeks. Mean anti-HBs titers in 68 children (29 females, 39 males) were 482.1 mlU/ml at six months after the third dose of primary vaccination and 153 mlU/ml at five years later. Total mean anti-HBs titers in 36 (52.9%) children out of 68 (17 females, 19 males) were 38.3 mlU/ml and 4(5.8%) of 68 children (two of each sexes) had no detectable antibody after five years. Total mean anti-HBs titers in these hypo- responder and non- responder were 774.3 mlU/ml and 625.5 mlU/ml respectively after booster dose. In a group of children, who were immunized with Cuban hepatitis B vaccine from birth, anti-HBS titers fell at 6.5 years of age and almost half of children became hypo responder or no responder and their anti-HBs titers developed secondary rise after booster vaccination. All children showed immunologic memory to a booster dose. (author)

  12. Detection of HBsAg and Anti HBc on donors of a blood bank by IRMA and ELISA methods

    International Nuclear Information System (INIS)

    Freire Martinez, D.Y.

    1985-10-01

    Comparative evaluation of two methods, Immunoradiometric Assay (IRMA) and Enzyme Immunoassay (ELISA), for detecting HBsAg and Anti HBc was made for determining which is the most advantageous and reliable. The study was made on 300 donors of the Hospital San Juan de Dios Blood Bank. In comparison with the reference method (IRMA), ELISA shows 91.67% of sensitivity. The Anti HBc detection by IRMA is more reliable than the HBsAg detection by IRMA and ELISA for determining the carrier state

  13. STUDY OF IMMUNISATION STATUS BY ESTIMATION OF ANTI-HBS ANTIBODY IN POST HEPATITIS B VACCINATED INDIVIDUALS

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    Karthik Pichika Lakshmanan

    2017-10-01

    Full Text Available BACKGROUND Hepatitis B Virus (HBV infection is a major public health problem in India. Hepatitis B can be prevented by hepatitis B vaccine, which is the first anticancer vaccine, because it can prevent a form of liver cancer. The protective antibodies induced by vaccination wane gradually over period of time. The aim of the study is to- 1. Estimate serum levels of anti-HBs in individuals vaccinated with hepatitis B vaccine. 2. Immunisation status of hepatitis B vaccination in individuals. MATERIALS AND METHODS A serological study was carried out from March 2015 to the end of September 2016 aimed at estimating the level of HBsantibody. Total of 330 individuals from healthcare workers, staff and children who have received full course of hepatitis B vaccine were selected for study. In a cross-sectional study, anti-HBs antibody was determined by Enzyme-Linked Immunosorbent Assay (ELISA method. RESULTS Three hundred and thirty individuals were enrolled in the study, out of which, 136 were men and 194 were women. Majority were in the age group 20 to 40 years. Anti-HBs antibody titre was more than 100 IU/L in 74% individuals. Titre was between 10 IU/L-100 IU/L in 16% individuals. Anti-HBs titre was less than 10 IU/L in 10% individuals. There was a significant decline in the levels of antibody overtime post vaccination. Antibody titre was low in individuals with diabetes mellitus. Low antibody titre was noted in smokers. CONCLUSION In this study, majority had desirable immune response to the HBV vaccine. Diabetes mellitus, long duration post vaccination and positive smoking history have attributed to low anti-HBs titre in subjects who had inadequate levels in our study. As immunological memory persists for long time even in the absence of significant titre of anti-HBs, booster dose vaccination is routinely not advocated for general population. But, healthcare professionals are advised to receive booster dose vaccination at 5 years if anti-HBs value is

  14. Detección de anticuerpos Anti-brucella en focas de Weddell (Leptonychotes weddellii) de Cabo Shirref, Antártica Detection of anti-brucella antibodies in Weddell seals (Leptonychotes weddellii) from cape Shirref, Antarctica

    OpenAIRE

    O. BLANK; P. RETAMAL; P. ABALOS; D. TORRES

    2002-01-01

    Posterior al hallazgo de anticuerpos anti-Brucella en muestras de lobo fino antártico (Arctocephalus gazella), el estudio serológico en Pinnipedia Antárticos se continuó con el fin de determinar la presencia de anticuerpos anti-Brucella en otras especies. Se colectaron muestras de sangre y de fluido extravascular de 12 ejemplares de foca de Weddell (Leptonychotes weddellii) encontrados en el Sitio de Especial Interés Científico (SEIC) Nº 32 y sitio CCAMLR Ecosystem Monitoring Program (CEMP) N...

  15. Serum Anti-Hbs-Ag in Stable Hemodialysis Patients and its Relationship with Various Demographic and Biochemical Data

    Directory of Open Access Journals (Sweden)

    Azar BARADARAN

    2010-12-01

    Full Text Available Introduction: To evaluate the relationship between various biochemical, nutritional and demographic factors with immune response to hepatitis B vaccine in maintenance hemodialysis (HD patients. Material and Methods: A retro-prospective study was carried out on 68 patients undergoing maintenance hemodialysis .Patients were vaccinated against hepatitis B virus with an intramuscular hepatitis B vaccination schedule, 40 micrograms at 0, 1, and 6 months. We also selected 32 age matched normal healthy persons who had vaccinated against hepatitis B previously to compare the antibody production with HD patients. Results: The value of serum antibody level against hepatitis B surface antigen ( HBs in hemodialisis patients and healthy persons were 35±55(median=5.5 and 135±71 (median=175 mIU/ml respectively. There was a significant deference between mean serum antibody level against HBs antigen of hemodialysis patients and normal subjects (p<0.001, there were not any significant differences of antibody production against HBs antigen between males and females or diabetic and non diabetics. There were no correlation between serum antibody level against HBs-Ag and serum albumin and also with body mass index. There were not significant correlation between anti-HBs antibody level and age, amounts of hemodialysis, duration of dialysis, dialysis adequacy, serum ferritin level and serum lipids. There were not also significant correlation between anti-HBs antibody level and serum parathormone, calcium, phosphorus, serum hemoglobin and hematocrit level. Conclusion: In this study, there was not significant correlation between serum antibody level against hepatitis B surface antigen and various nutritional and demographic factors of patients under regular hemodialysis.

  16. Monitoring of post-vaccination anti-HBs titles vaccine in children and adolescents in the pre-dialysis of chronic kidney disease

    Directory of Open Access Journals (Sweden)

    Nathália Cristina Pereira da Costa

    2017-08-01

    Full Text Available Abstract Introduction: Bacterial or viral diseases are one of the major causes of death in patients with chronic kidney disease (CKD. These patients show a quantitative reduction of levels of antibodies over time. Among the infectious diseases that affect CKD patients, stands out hepatitis B (HB. Immunization and control of antibodies levels against the hepatitis B surface antigen (anti-HBs are ways to prevent the HB infection in this population. Patients with anti-HBs levels ≥10 IU/ml are considered adequate responders, whereas those with anti-HBs levels ≥ 100 IU/ml are considered excellent responders. Objective: To analyze the variation of the anti-HBs levels obtained after vaccination against HB in children and adolescents in the pre-dialysis stage of CKD. Methods: A retrospective cohort study on anti-HBs levels of children and adolescents in the pre-dialysis stage of CKD. Correlation between levels of anti-HBs titers and time since the vaccination were estimated. Results: From the total of 116 studied patients most of the studied patients were considered excellent responders, obtaining in the three anti-HBs titers percentages of 70.7%, 62.1% and 54.9% respectively. The anti-HBs titer levels showed a negative correlation with the time since vaccination (Kendall Tau-b = -0.16; p = 0.02. Conclusion: The majority of the studied population was vaccinated by PNI and showed excellent anti-HBs titer levels, even experiencing a progressive reduced response over the time.

  17. Evaluación de la presencia de anticuerpos anti-Toxoplasma gondii y anti-Neospora caninum en sueros caninos de la provincia de Buenos Aires mediante las técnicas de inmunofluorescencia indirecta y aglutinación directa

    OpenAIRE

    Gos, María Laura

    2016-01-01

    Objetivos generales: Determinar la presencia de anticuerpos anti-T. gondii y anti-N. caninum en caninos de la provincia de Buenos Aires por la técnica de inmunofluorescencia indirecta y evaluar una prueba de aglutinación comercial para la detección de anticuerpos anti-T. gondii en caninos domésticos. Objetivos específicos: 1. Determinar la presencia de anticuerpos anti-T. gondii y anti-N. caninum en caninos provenientes de la provincia de Buenos Aires en el perí...

  18. Anticuerpos IgG anti-toxoplasma gondii en pacientes con síntomas atribuíbles a toxoplasmosis

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    Jorge E. Collazo

    1993-12-01

    Full Text Available Se evaluaron 6.520 pacientes con síntomas atribuíbles a toxoplasmosis por la presencia de anticuerpos IgG anti - Toxoplasma gondii mediante la técnica de inmunofluorescencia indirecta. El 51,27% de los evaluados resultaron positivos.El 60,85% de los pacientes con trastornos oculares son seropositivos a IgG anti - T. gondii; seguidos del 56,25 y 48,10% para aquellos con trastornos generales y gineco-obstétricos. Los síntomas o manifestaciones que evidenciaron mayor porcentaje de positividad fueron: astenia, coriorretinitis, trastornos menstruales, cefaleas y uveítis.

  19. Decrease in Anti-HBs Antibodies over Time in Medical Students and Healthcare Workers after Hepatitis B Vaccination

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    H. V. Sahana

    2017-01-01

    Full Text Available Background. Hepatitis B is one of the most important occupational hazards among healthcare workers (HCWs. This study aimed to measure the anti-HBs titres among the medical students and HCWs vaccinated against hepatitis B virus and to determine the association between anti-HBs levels and time since vaccination. Materials and Methods. In this cross-sectional study, medical students and healthcare workers who had received all three doses of hepatitis B vaccination and completed at least six months after vaccination since the last dose were included. 3 ml blood was collected from subjects (n=340 and anti-HBs titre was estimated using ELISA. Results. A total of 340/400 subjects were aged between 18 and 60 years; 204 were females and 136 males. The median and interquartile range for time since vaccination were 5 and 5 years, respectively. Duration since vaccination was ≤5 years in 223 (65.5%, 6–10 years in 84 (24.7%, and >10 years in 33 (9.70%; among them, antibody titres were >10 mIU/ml in 94.1%, 79.7%, and 72.7% subjects, respectively. There was significant decline in antibody titres as duration of postvaccination increased. Conclusion. The proportion of subjects who were unprotected after 5 and 10 years after vaccination were 20% and 27%, respectively. The need for a booster dose can be made mandatory at least for healthcare professionals.

  20. Awareness and Practice of Complete Hepatitis B Vaccination and Anti-HBs Testing in Vaccinated Health Care Workers

    Directory of Open Access Journals (Sweden)

    Annapurna G. Sajjan

    2015-04-01

    Full Text Available Background: Hepatitis B is a serious and common infectious disease affecting millions of people worldwide. Health Care Workers (HCW are at an increased risk of occupational exposure to HBV and the incidence is 2-4 times higher than in the general population. Despite potential risks, awareness and vaccine compliance is poor among the HCWs. Aim: To assess the awareness of complete Hepatitis B vaccination, anti-HBs testing & protective titres and determine the anti HBs titres amongst vaccinated HCWs. Material & Methods: A total of 500 Health care workers of both sexes in the age group from 20- 60 years vaccinated against Hepatitis B were tested for anti-HBs titres by quantitative ELISA. Results: The rate of complete immunization was 81.4% in doctors, 63.3% in nursing staff and 90% in the technical staff. Amongst the 500 participants, 70.8% had received all the doses and 29.2% incomplete doses of the vaccine. Titres of ≥ 10 mIU/ml were demonstrated in 84.4% of HCWs who received all the doses and in 65.7% those who defaulted. Conclusions: The results of the study indicate lack of awareness about complete HB vaccination and the importance of post vaccination testing in HCWs.

  1. The one year effects of three doses of hepatitis B vaccine as a booster in anti-HBs-negative children 11-15 years after primary immunization; China, 2009-2011.

    Science.gov (United States)

    Yao, Jun; Shan, Huan; Chen, Yongdi; Jiang, Zheng-gang; Dai, Xue-wei; Ren, Jing-jing; Xu, Kai-jin; Ruan, Bing; Yang, Shi-gui; Li, Qian

    2015-01-01

    The aim of this study was to evaluate hepatitis B surface antibody (anti-HBs) levels one year after hepatitis B booster vaccination in anti-HBs-negative (vaccination. Anti-HBs titers were examined in 235 children who were negative for hepatitis B surface antigen (HBsAg), anti-HBs, and hepatitis B core antibody (anti-HBc). The children were then divided into 3 groups based on their anti-HBs levels pre-booster: Group I, vaccinated with 3 doses of hepatitis B vaccine (0-1-6 month, 20 ug), and anti-HBs levels were measured. One month after the first dose, the anti-HBs positive rates (≥ 10 mIU/mL) in Groups I-III were 56.14%, 83.61% and 100%. One month after the third dose, the anti-HBs-positive rates in Groups I-III were 96.49%, 98.36% and 100%. One year after the third dose, the anti-HBs-positive rates in Groups I-III were 73.68%, 75.41% and 98.29%, respectively. Protective levels declined more rapidly for those with lower titers. Children with pre-booster anti-HBs titers of 1-9.9 mIU/mL might not need any booster dose, and the children with pre-booster titers of 0.1-0.9 and vaccination.

  2. [Anti-HBs persistence after revaccination with three doses of hepatitis B vaccine among low-responder infants following primary vaccination: 4-year of follow-up].

    Science.gov (United States)

    Lyu, J J; Yan, B Y; Liu, J Y; Feng, Y; Wu, W L; Liang, X F; Cui, F Q; Wang, F Z; Zhang, G M; Xu, A Q; Zhang, L

    2017-06-06

    Objective: Assess the 4-year antibody against hepatitis B surface antigen (anti-HBs) persistence after revaccination with 3-dose of hepatitis B vaccine (HepB) among low-responder infants following primary vaccination. Methods: According to stratified cluster sampling, a total of 4 147 infants were enrolled and primarily vaccinated with 5 μg HepB derived in Saccharomyces Cerevisiae (HepB-SC) at 0-1-6 months schedule from 75 towns of Jinan, Weifang, Yantai, Weihai prefectures, Shandong Province, China in Aug and Sep 2009. Blood samples were collected one to six months after the third dose of primary immunization and tested for anti-HBs using chemiluminescence microparticle immunoassay (CMIA). 717 infants who appeared low response (10 mU/ml ≤ anti-HBsvaccination were collected. The risk factors associated with positive rate of anti-HBs and GMC of anti-HBs were identified by multiple non-conditional logistic regression analysis and multifactor linear regression model analysis, respectively. Results: Among 315 children, 165 (52.38%) were male and 150 (47.62%) were female. The positive rate was 83.81% (264/315) at T(0) and it decreased to 16.51% (149/529) at T(1). The corresponding GMC decreased from 473.15 mU/ml to 17.37 mU/ml. The average annual decreasing rate of positive rate and GMC was 33.38% and 56.23% from T(0) to T(1). Multivariable analysis showed the positive rate and GMC among those whose anti-HBs titer higher at T(0) were significantly higher at T(1). The positive rate at T(1) among those whose anti-HBs titer 400-low-responder infants, but still kept good protection. The anti-HBs persistence after revaccination was associated with anti-HBs level of titer one month after revaccination.

  3. Preliminary Assessment of Various Additives on the Specific Reactivity of Anti- rHBsAg Monoclonal Antibodies

    Science.gov (United States)

    Yazdani, Yaghoub; Mohammadi, Saeed; Yousefi, Mehdi; Shokri, Fazel

    2015-01-01

    Background: Antibodies have a wide application in diagnosis and treatment. In order to maintain optimal stability of various functional parts of antibodies such as antigen binding sites, several approaches have been suggested. Using additives such as polysaccharides and polyols is one of the main methods in protecting antibodies against aggregation or degradation in the formulation. The aim of this study was to evaluate the protective effect of various additives on the specific reactivity of monoclonal antibodies (mAbs) against recombinant HBsAg (rHBsAg) epitopes. Methods: To estimate the protective effect of different additives on the stability of antibody against conformational epitopes (S3 antibody) and linear epitopes (S7 and S11 antibodies) of rHBsAg, heat shock at 37°C was performed in liquid and solid phases. Environmental factors were considered to be constant. The specific reactivity of antibodies was evaluated using ELISA method. The data were analyzed using SPSS software by Mann-Whitney nonparametric test with the confidence interval of 95%. Results: Our results showed that 0.25 M sucrose, 0.04 M trehalose and 0.5% BSA had the most protective effect on maintaining the reactivity of mAbs (S3) against conformational epitopes of rHBsAg. Results obtained from S7 and S11 mAbs against linear characteristics showed minor differences. The most efficient protective additives were 0.04 M trehalose and 1 M sucrose. Conclusion: Nowadays, application of appropriate additives is important for increasing the stability of antibodies. It was concluded that sucrose, trehalose and BSA have considerable effects on the specific reactivity of anti rHBsAg mAbs during long storage. PMID:26605008

  4. Vasculitis asociadas a anticuerpos anti-citoplasma de neutrófilos: Clínica y tratamiento

    Directory of Open Access Journals (Sweden)

    María Virginia Paolini

    2013-04-01

    Full Text Available Las vasculitis asociadas a anticuerpos anti-citoplasma de neutrófilos (ANCA comprenden a un grupo de enfermedades caracterizadas por la inflamación de la pared de pequeños vasos. Analizamos las características epidemiológicas y clínicas en una serie de 47 pacientes: 23 (49% granulomatosis de Wegener (GW, 15 (32% poliangeítis microscópica (PAM y nueve (19% vasculitis limitada al riñón (VLR. La edad media al inicio de los síntomas fue de 50.7 ± 14.9 años. La manifestación clínica más frecuente fue el compromiso renal en 41 (87% pacientes, seguido por el pulmonar en 26 (55% y el otorrinolaringológico en 17 (36%. En 26 (55% se asoció compromiso renal y pulmonar. La forma clínica más frecuente fue la generalizada en 23 (49%, seguida por la grave en 18 (38%. El 89% presentaron determinaciones de ANCA positivas. Cuatro (8% no recibieron tratamiento inmunosupresor de inicio. De los 43 que recibieron tratamiento de inicio, 29 (67% tuvieron remisión completa, con un tiempo de remisión promedio de 35.3 meses. Once (26% presentaron recaídas, diez (91% recaídas mayores y uno (9% menor. Doce (28% fallecieron, siete en forma temprana y cinco durante la evolución de la enfermedad. Quince (31% evolucionaron a insuficiencia renal crónica. Los 26 pacientes en seguimiento tuvieron respuesta al tratamiento y 20 (77% de ellos estaban en remisión al finalizar el estudio. Las vasculitis asociadas a ANCA continúan siendo enfermedades de alta morbilidad y mortalidad, a pesar de las mejorías logradas con los tratamientos inmunosupresores.

  5. [Retrospective evaluation of HBsAg, anti-HCV, anti-HIV and syphilis reagin antibody seropositivity in blood donors at the Trabzon Farabi Hospital].

    Science.gov (United States)

    Aydin, Faruk; Cubukçu, Kivanç; Yetişkul, Serpil; Yazici, Yelda; Kaklikkaya, Neşe

    2002-01-01

    Transfusion of blood and blood products is a widely used method for therapy in medicine, however it may result with the transmission of infectious agents from donor to recipient. In order to achieve safe blood transfusions and to minimize post-transfusion infections, several screening tests for infectious agents are routinely done all around the world as well as in our country. In this study, HBsAg, anti-HCV, anti-HIV and syphilis reagin antibody tests results have been retrospectively evaluated for 33.766 blood donors during January 1, 1997 to December 31, 2000 in Blood Center of Farabi Hospital, Black Sea Technical University. Testing for HBsAg, anti-HIV and anti-HCV has been done by using commercially available micro and/or macro enzyme immunoassays, and syphilis reagin antibody test by latex agglutination (RPR) method. The indeterminate results were confirmed by retesting of sera with microparticle enzyme immunoassay and Western blot methods. As a result, in 1331 (3.94%) subjects HBsAg, in 250 (0.74%) subjects anti-HCV, and in 161 (0.47%) subjects RPR were found positive. Twenty samples which have had the results in gray-zone for anti-HIV, have been found negative with the confirmation tests.

  6. Anticuerpos anti 21 hidroxilasa séricos en pacientes con anticuerpos antifracción microsomal: Síndrome poliendocrino autoinmune Seric 21- hydroxilase antibodies in patients with anti-microsomal fraction antibodies: Autoimmune polyendocrine syndrome

    Directory of Open Access Journals (Sweden)

    Silvia Botta

    2007-04-01

    Full Text Available El síndrome poliendocrino autoinmune (SPA es la asociación de enfermedades endocrinas autoinmunes con otros desórdenes autoinmunes no endocrinos. Los tipos 1, 2 y 4 presentan adrenalitis autoinmune, esto indica la presencia de autoanticuerpos, y su marcador serológico específico es el anti 21 hidroxilasa (a21-OH. El SPA tipo 2 es la asociación de adrenalitis, enfermedad tiroidea y/o diabetes mellitus inducidas por autoanticuerpos. Como componentes menores, pueden estar asociados entre otros, vitiligo, alopecia y miastenia. Nuestros objetivos fueron: establecer la prevalencia de a21-OH séricos en pacientes con anticuerpos anti fracción microsomal (AFM positivos, enfermedad tiroidea autoinmune y/o afecciones endocrinas y no endocrinas autoinmunes; diagnosticar formas incompletas de SPA y estudiar individuos con probable riesgo de progresión a un SPA completo. Estudiamos 72 pacientes AFM positivos y 60 sujetos tomados como grupo control, AFM negativos. Hallamos a21-OH elevados en dos pacientes: A= 47 U/ml, hipotiroidismo autoinmune y miastenia; y B= 8.75 U/ml, hipotiroidismo autoinmune y vitiligo; ambos con ausencia de insuficiencia adrenal. La prevalencia de a21-OH encontrada fue del 2.8%. Las pacientes A y B corresponden a un SPA tipo 2 incompleto y latente en relación al componente adrenal. Considerando a los a21-OH marcadores de enfermedad autoinmune latente, el eventual riesgo de evolución hacia la afección clínica sugiere la necesidad de estrechos controles clínicos y bioquímicos periódicos.Autoimmune polyendocrine syndrome (APS is the association of autoimmune endocrine diseases, with other autoimmune nonendocrine disorders. APS types 1, 2 and 4 include autoimmune adrenalitis; this suggests the presence of autoantibodies. A specific serological marker for these is the anti 21- hydroxilase autoantibody (a21-OH. APS type 2 is the association of autoimmune adrenalitis, to autoimmune thyroid disease and/or diabetes mellitus, all

  7. Triple positivity of HBsAg, anti-HCV antibody, and HIV and their ...

    African Journals Online (AJOL)

    Background: Few studies exist on hospital-based seroprevalence of triple positivity of HIV/HBV/HCV in Nigeria. Objectives: The study aimed at determining the triple positivity of HIV, HBsAg and HCV among HIV-infected individuals in Abeokuta, Nigeria and defining the influence of these triple infections on CD4+ counts of ...

  8. The association of the vitamin D status with the persistence of anti-HBs antibody at 20years after primary vaccination with recombinant hepatitis B vaccine in infancy.

    Science.gov (United States)

    Jafarzadeh, A; Keshavarz, J; Bagheri-Jamebozorgi, M; Nemati, M; Frootan, R; Shokri, F

    2017-02-01

    Vitamin D has potent immunoregulatory effects due to the expression of its receptor on the majority of immune cells. The aim was to evaluate the association of the vitamin D status with the persistence of anti-HBs antibody and immune response to booster immunization at 20years after primary vaccination with hepatitis B (HB) vaccine. Blood samples were collected from 300 adults 20years after completion of the primary HB vaccination in infancy. The serum levels of vitamin D and anti-HBs antibody were measured by ELISA. A single booster dose of a recombinant HB vaccine was administered to a total of 138 subjects, whose anti-HBs titer wasanti-HBs antibody, 4weeks after booster vaccination. At 20years after primary vaccination, the mean vitamin D concentrations were significantly higher in seroprotective subjects as compared to non-seroprotective individuals (Panti-HBs were significantly increased with advanced concentrations of vitamin D (PD were significantly higher in subjects with an anamnestic response to booster vaccination as compared with subjects without this response (PD status may influence the persistence of anti-HBs antibody and durability of protection after primary vaccination with a recombinant HB vaccine in infancy. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  9. Age-dependent decrease of anti-HBs titers and effect of booster doses using 2 different vaccines in Palestinian children vaccinated in early childhood

    Science.gov (United States)

    Qawasmi, Mohammad; Samuh, Monjed; Glebe, Dieter; Gerlich, Wolfram H; Azzeh, Maysa

    2015-01-01

    Immunization against hepatitis B virus (HBV) has proven to be highly effective and led to significant reduction of new infections worldwide. However, protective immunity measured by anti-HBs titers may decrease to critical levels in the years after basal immunization, particularly in case of exposure to HBV variants different from the vaccine strain. We tested 400 Palestinian children between one and 19 years of age for their anti-HBs titer, challenged the immune memory of those with low or absent anti-HBs with 2 types of hepatitis B vaccines and determined thereafter the anti-HBs titer. At the age of one, 92.2% of the children presented with protective anti-HBs titers (≥10 mIU/ml) with the majority having ≥100 mIU/ml. Protective immunity was still high at ages 2 (87.5%) and 4 (95%), declining by age 5 and 6 (from 69.2% to 66.7%) and down to an average of 39.8% between the ages of 7 and 19. 160 children with a nonprotective or low immune response challenged with either the yeast-derived Engerix-B or the mammalian cell-derived preS1-containing Sci-B-Vac vaccine showed an anamnestic immune response. 92.4% and 85.9% of the children challenged with one dose Sci-B-Vac and Engerix-B presented with anti-HBs titers >100 mIU/ml respectively. Our results reveal that vaccine-induced protective anti-HBs titers against HBV decrease rapidly beyond the age of 6 in Palestinian children, but can be strongly enhanced with a single booster vaccine dose, independent of brand and antigen composition. Our data suggest that a booster vaccine dose against HBV during school years may be useful. PMID:25996579

  10. Producción de anticuerpos IgG1 anti-glucuronoxilomanana en ratones resistentes a la criptococosis

    Directory of Open Access Journals (Sweden)

    Claudia Parra

    2005-03-01

    Full Text Available lntroducción. Cryptococcus neoformans es un hongo ubicuo que está relacionado con infecciones oportunistas en pacientes inmunocomprometidos, especialmente los pacientes infectados con el virus de la inmunodeficiencia humana. C. neoformans posee una cápsula que contiene principalmente glucuronoxilomanana que actúa como factor de virulencia y es considerado como antígeno timo independiente tipo-2. Objetivos. Determinar la cinética de producción de las subclases de lgG específicas para la glucuronoxilomanana, medir los anticuerpos de reactividad cruzada contra el polisacárido de Streptococcus pneumoniae y determinar las subpoblaciones esplénicas de linfocitos en tres modelos de criptococosis en ratones con diferentes susceptibilidades a la infección. Materiales y métodos. Los anticuerpos fueron detectados por la técnica inmunoenzimática ELlSA después de la infección con C. neoformans en ratones moderadamente resistentes (Balb/c, resistentes (CBA/j y susceptibles (C57BL/6. Las subpoblaciones de linfocitos B se determinaron por citometría de flujo. Resultados. Se observó la producción temprana de anticuerpos lgG que coincidía con una disminución en el número de unidades formadoras de colonias de C. neoformans en pulmón. Los anticuerpos de reactividad cruzada se detectaron en las tres cepas de ratones después de la infección; fueron mayores en la cepa susceptible C57BL/6, la cual mostró el porcentaje más alto de linfocitos B, CD5+ en el bazo. En contraste, los ratones CBA/J presentaron los mayores niveles de linfocitos B, CD43+. Conclusiones. Estos hallazgos sugieren que los anticuerpos lgG específicos para la glucuronoxilomanana están implicados en la protección del hospedero contra la infección por C. neoformans y podrían estar regulados por células CD43+. También sugiere que los anticuerpos de reactividad cruzada no poseen un papel relevante en el control de la infección.

  11. Soroprevalência e fatores de risco para a infecção pelo vírus da hepatite B pelos marcadores AgHBs e anti-HBs em prisioneiros e primodoadores de sangue Seroprevalence and risk factors for hepatitis B by AgHBs and anti-HBs markers in prisoners and first-time donors, in Central Brazil

    Directory of Open Access Journals (Sweden)

    Celina Maria Turchi Martelli

    1990-08-01

    Full Text Available Foram rastreados 1.033 primodoadores de sangue e 201 prisioneiros, para a infecção pelo vírus da hepatite B (VHB, durante o período de junho de 1988 a fevereiro de 1989, em Goiânia, GO (Brasil. Foi estimada a soroprevalência dos marcadores AgHBs e anti-HBs, e estudados os fatores de risco associados a soropositividade. Os testes sorológicos foram realizados pela técnica de ELISA e a presença de qualquer dos marcadores estudados foi interpretada como exposição ao vírus da hepatite B. Um questionário padronizado foi aplicado em ambos os grupos populacionais para avaliar: transfusão sangüínea prévia, número de parceiros sexuais, atividade homo/bissexual, história de outras doenças sexualmente transmissíveis, uso de droga injetável, uso de medicação injetável, acupuntura, tatuagem e soropositividade ao VDRL. Foram obtidas soroprevalências globais para a infecção pelo VHB de 26,4% e 12,8% para a população carcerária e de primodoadores respectivamente, diferença estatisticamente significante (pTwo cross-sectional surveys on hepatitis B virus (HBV infection were carried out among 1,033 volunteer first-time blood donors in five blood banks (3 private, 2 public and among 201 prisoners in the Penitentiary Center of Industrial Activity, in Goiânia, Central Brazil, between June 1988 and February 1989. Those surveys were part of a major study designed to estimate seroprevalence of HBsAg and anti-HBsAg markers by ELISA test, and to study risk factors associated with seropositivity. The presence of any serum marker was considered as previous exposure to HBV. A standard questionnaire was applied to both populations to evaluate previous blood transfusion, number of sexual partners, homo/bisexual activity, history of sexually transmitted diseases, drug abusers, use of parenteral medicine, accupunture, tattooing and VDRL seropositivity. Seroprevalence varied from 12.8% to 26.4% in blood donors and prisoners, respectively, (p<0

  12. The Serum Anti-HBs Level Among Children Who Received Routine Hepatitis B Vaccination During Infancy in Mianyang City, China: A Cross-Sectional Study.

    Science.gov (United States)

    He, Fang; Ma, Yuan-ji; Zhou, Tao-you; Duan, Jin-chao; Wang, Jun-feng; Ji, Yu-lin; Li, Hong; Zhang, Ju-ying; Tang, Hong

    2016-01-01

    Hepatitis B virus (HBV) prevalence has declined remarkably in children due to nationwide universal vaccination program for HBV in China. However, the persistence of immune response against HBV infection and the optimal time point when a booster vaccination should be performed remain to be elucidated. To assess the persistence and level of antibody against hepatitis B surface antigen (anti-HBs) in a representative population of age 15 and younger who received routine hepatitis B vaccination in Mianyang City, China. A cross-sectional study was conducted in 2011. One thousand five hundred twenty-six children of age 15 and younger who received three doses of 5 μg hepatitis B vaccine series during infancy but did not receive a booster vaccination later were enrolled. Of the 1,526 children, the mean age was 8.2 ± 4.1 and 739 children were male. The median anti-HBs level was 23.0 mIU/mL, and the total percentage of anti-HBs levels ≥10 mIU/mL was 60.9%. With an increase of age, median anti-HBs level, percentage of anti-HBs levels ≥10 mIU/mL, and percentage of anti-HBs levels ≥100 mIU/mL declined remarkably in the early period and reached the lowest level at the age of 3 and then remained relatively stable. The median anti-HBs level, the percentage of anti-HBs levels ≥10 mIU/mL, and the percentage of anti-HBs levels ≥100 mIU/mL in 1- and 2-year-old children were much higher than that in children aged 3-15 (p children of 15 and younger who received three doses of 5 μg hepatitis B vaccine series during infancy in China. Three dosages of 10 μg hepatitis B vaccine for infants and repeated vaccination or additional booster vaccination for some children at or before age 3 should be provided to get much more powerful immunity to HBV.

  13. Asociación entre variables reproductivas y anticuerpos anti Neospora caninum en bovinos lecheros de un municipio de Colombia

    OpenAIRE

    Martín Orlando Pulido Medellín; Adriana María Díaz Anaya; Roy José Andrade Becerra

    2017-01-01

    El objetivo fue determinar el porcentaje de vacas seropositivas a Neospora caninum y la identificación de las variables reproductivas que se encuentran relacionadas con la presencia de anticuerpos contra este protozoo. Se tomaron 1,000 muestras de sangre a hembras bovinas del municipio de Sotaquirá, Boyacá, las cuales fueron evaluadas por medio de ELISA indirecta. Se determinó una seroprevalencia de Neospora caninum del 45 %; las variables reproductivas asociadas con la positividad de N. cani...

  14. Protection status against hepatitis B infection assessed fromanti-HBs level, history of vaccination andhistory of infection based on anti-HBc in medical students

    Science.gov (United States)

    Annisa; Zain, LH; Loesnihari, R.

    2018-03-01

    Hepatitis B virus (HBV) is one of the most contagious pathogens where the risk of exposure is very high among health care workers, especially students in the clerkship. This study describes the protection status by measuring anti-HBs level, history of vaccination, and history of HBV infection in medical students.Forty-four (44) students over 18 years old were randomly selected, interviewed for their vaccination history and then had their blood serum taken for anti-HBs and anti-HBc examinations to determine the protectivity and history of infection.There were 81.8% students without a protective anti-HBs level. Before starting their clerkship, 18.2% students received thevaccination, and only one-fourth formed protective antibody level above 10mIU/mL. Seventeen (38.6%) students had been exposed to HBV(positive anti-HBc), and only six of them showed protective anti-HBs level. None of the students that received vaccine underwent a post-vaccination serological test (PVST) to determine their immune response. These results indicated the vulnerability of medical students to the risk of HBV transmission while performing medical care. With the high incidence of HBV transmission, educational institutions are encouraged to make provisions for vulnerable students to receive a booster and an adequate PVST before their clerkship.

  15. Desarrollo de superficies modificadas oro - anticuerpo anti-PSPB para su uso como biosensor en la detección de preñez bovina

    Directory of Open Access Journals (Sweden)

    Justo Laíz

    2011-05-01

    Full Text Available En este trabajo se presentan los resultados preliminares para el desarrollo de un biosensor amperométrico destinado a la cuantificación de Proteína B Específica de la Preñez (PSPB en fluidos biológicos como medio de diagnóstico clínico precoz de la preñez bovina. A la superficie de oro modificada con ácido mercaptoundecanoico (MUA se fijaron anticuerpos anti-PSPB y peroxidasa de rábano (HRP. Se verificó la inmovilización de las biomoléculas mediante voltamperometría cíclica y medida del ángulo de contacto.Se estudió el comportamiento electroquímico del electrodo desarrollado por medio de voltamperometría cíclica y medidas a potencial constante, estableciendo la relación entre el cambio de corriente catódica (al potencial de reducción de la quinona y la concentración de PSPB. Esta relación se ve afectada por la formación de complejos antígeno – anticuerpo que limitan el acceso del sustrato al sitio activo de la enzima, provocando una disminución en la señal eléctrica.El electrodo modificado de oro - anti-PSPB – HRP respondió de forma inversamente proporcional a la concentración de PSPB a un potencial de -200 mV (contra electrodo de referencia Ag/AgCl, tanto por voltamperometría cíclica como a potencial constante, y por tanto podría ser la base para el desarrollo de un biosensor amperométrico para detectar precozmente la preñez bovina.

  16. [Anti-HBs persistence following revaccination with three doses of hepatitis B vaccine among low-responsive adults after primary vaccination: a 4-year follow-up study].

    Science.gov (United States)

    Lyu, J J; Yin, X W; Yan, B Y; Liu, J Y; Feng, Y; Wu, W L; Chen, S Y; Zhou, L B; Liang, X F; Cui, F Q; Wang, F Z; Zhang, L; Xu, A Q

    2016-06-01

    To assess the 4-year anti-HBs persistence after revaccination with 3-dose of hepatitis B vaccine (HepB) among low-responsive adults. A total of 24 237 healthy adults who had no history of hepatitis B infection and hepatitis B vaccination, resided in the local area for more than six months and were aged 18-49 years were selected from 79 villages of Zhangqiu county, Shandong province, China in 2009. Blood samples were obtained and hepatitis B surface antigen (HBsAg), antibody against hepatitis B surface antigen (anti-HBs) and antibody against hepatitis B core antigen (anti-HBc) were detected using ELISA method. A total of 11 590 persons who were negative for all of these indicators were divided into four groups by cluster sampling method. Each group was vaccinated with one of the following four types of HepB at 0-1-6 months schedule: 20 μg HepB derived in Saccharomyces cerevisiae (HepB-SC), 20 μg HepB derived in Chinese hamster ovary cell (HepB-CHO), 10 μg HepB-SC and 10 μg HepB derived in Hansenula polymorpha (HepB-HP). Blood samples were collected one month after the third dose of primary immunization and tested for anti-HBs using chemiluminescence microparticle immunoassay (CMIA). The 892 low-responders were revaccinated with three doses of HepB at 0-1-6 months schedule and the type of HepB was the same as which was used for primary immunization. During the follow-up to low-responders, the following informations were collected: the demographic characteristics (including age, gender), histories of hepatitis B infection, hepatitis B vaccination, smoking, drinking and chronic diseases. Blood samples were collected one month (T1) and four years after revaccination and anti-HBs, anti-HBc and HBsAg (if anti-HBs low-responders. Among 529 participants, 276 (52.2%) were males and 253 (47.8%) were females. The positive rate was 82.6% (437/529) at T1 and it decreased to 28.2% (149/529) four years after revaccination. The corresponding GMC decreased from 542.06 (95% CI

  17. Determinación de anticuerpos anti-β2glicoproteína I en pacientes con síndrome antifosfolípido Anti- β2 glycoprotein antibodies in patients with antiphospholipid syndrome

    Directory of Open Access Journals (Sweden)

    Oscar Uribe Uribe

    2004-09-01

    Full Text Available EL objetivo de este estudio fue comparar la presencia de anticuerpos anti-β2glicoproteína I (anti- β2GPI con las pruebas convencionales de laboratorio de anticuerpos anticardiolipina (aCL y anticoagulante lúpico, y con las manifestaciones clínicas del síndrome antifosfolípido (SAF. Se incluyeron en el estudio 80 mujeres con SAF; 35 de ellas de la consulta de Reumatología y las otras 45 con historia de aborto recurrente espontáneo (ARE; 5 mujeres de la consulta de Reumatología sin SAF, 27 mujeres con ARE, sin SAF y un grupo control de 20 mujeres sanas en edad reproductiva. Se investigaron la presencia de anticuerpos IgG e IgM anticardiolipina (aCL e IgG anti- β2GPI por la técnica de ELISA, y el anticoagulante lúpico por la determinación del tiempo parcial de tromboplastina activado. Adicionalmente, se registraron las manifestaciones clínicas asociadas al SAF. De las pacientes con SAF, 25.7% del grupo de Reumatología (9/35 y 4.4% de las pacientes con ARE (2/45 fueron positivas para anticuerpos anti-β2GPI, mientras que ninguna de las mujeres sin SAF, ni de las mujeres del grupo control, fue positiva. La asociación entre la presencia de anti- β2GPI y los anticuerpos IgG e IgM aCL mostró una diferencia significativa en los títulos de 3+ (altamente positivos en contraste con los individuos negativos para anti- β2GPI. La positividad del anticoagulante lúpico también se correlacionó con la presencia de anticuerpos anti- β2GPI. No hubo diferencia significativa entre las diversas manifestaciones clínicas del SAF y la presencia de dichos anticuerpos. En conclusión, la determinación de anticuerpos anti- β2GPI tiene una alta especificidad en pacientes con SAF pero no se asoció con ninguna manifestación clínica en particular. The objective of this study was to compare the presence of anti-β2glycoprotein (anti- β2GPI antibodies with the conventional laboratory tests of anticardiolipin antibodies (aCL and lupus anticoagulant

  18. Frequency of hepatitis B surface antibody (anti-HBs) in various Canadian populations as measured by modified solid-phase radioimmunoassay

    International Nuclear Information System (INIS)

    Bishai, F.R.; MacMillan, S.; Rhodes, A.J.; Dempster, G.; Spence, L.; Wrobel, D.M.

    1977-01-01

    A short incubation solid-phase radioimmunoassay test was developed and used for the detection of hepatitis B surface antibody (anti-HBs) in the sera collected from patients recovering from hepatitis B infection, health care personnel, staff and residents of an intstitution for the mentally retarded and in pregnant and non-pregnant women in Ontario. (author)

  19. Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix Association between anti-Ras and anti-HPV16 E4/E7 antibodies with cervical intraepithelial lesions

    Directory of Open Access Journals (Sweden)

    Sara Vázquez-Corzo

    2003-10-01

    Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.OBJECTIVE: To evaluate whether serum antibodies anti-E4, E7 and Ras could be used as markers for early cervical lesions associated with HPV (human papillomavirus. MATERIAL AND METHODS: A seroepidemiological case-control study was conducted between March 1999 and April 2000 at the dysplasia clinic of Hospital General Doctor Gea Gonzalez, in Mexico City, to evaluate the presence of antibodies anti-E4, E7, and Ras through a sandwich ELISA. Analysis was done using odds ratios and 95% confidence intervals. RESULTS: Anti-E7 antibodies were associated to women with CIN III lesions, while anti-E4 and Ras antibodies were strongly associated with CIN I-II lesions. The antibody profile of women with different

  20. Effect of revaccination using different schemes among adults with low or undetectable anti-HBs titers after hepatitis B virus vaccination.

    Science.gov (United States)

    Lin, Chao-Shuang; Xie, Shi-Bin; Liu, Jing; Zhao, Zhi-Xin; Chong, Yu-Tian; Gao, Zhi-Liang

    2010-10-01

    Our objective was to investigate the effect of various reimmunization schemes for hepatitis B in adults with low or undetectable anti-HBs titers. Over 2 years, 10 μg of Saccharomyces cerevisiae-recombinant hepatitis B virus (HBV) vaccine (synthesized in China) was used in at least one standardized scheme to immunize 2,310 healthy male and nonpregnant female adults. Of these, 240 subjects tested negative for hepatitis B markers. These 240 subjects were equally divided into 4 groups. The first group, designated Engerix-40, was revaccinated with 40 μg Engerix-B; the second, Engerix-20, was revaccinated with 20 μg Engerix-B; the third, Chinese-20, was revaccinated with 20 μg Chinese-made yeast-recombinant vaccine; and the last group, Chinese-10, was revaccinated with 10 μg Chinese-made yeast-recombinant vaccine. Blood samples were collected before and 1, 2, 8, and 12 months after the first injection. The anti-HBs-positive conversion rates of the Engerix-40, Engerix-20, and Chinese-20 groups were higher than that of the Chinese-10 group (P anti-HBs conversion rate increased in all groups, but values were significantly different from those for the other groups only in the Chinese-10 group (P anti-HBs geometric mean titers (GMTs) of the Engerix-40, Engerix-20, and Chinese-20 groups were higher than in the Chinese-10 group (P anti-HBs titers in subjects with low or undetectable titers after HBV vaccination.

  1. Long term persistence of anti-HBs protective levels in young patients with type 1 diabetes after recombinant hepatitis B vaccine.

    Science.gov (United States)

    Marseglia, G; Alibrandi, A; d'Annunzio, G; Gulminetti, R; Avanzini, M A; Marconi, M; Tinelli, C; Lorini, R

    2000-11-22

    The aim of the present study was to evaluate the persistence of anti-hepatitis B protective levels in young patients with type 1 diabetes, successfully immunised with a recombinant hepatitis B vaccine. We re-evaluated, after a 4 year follow-up, 54 patients and 70 age and sex-matched healthy subjects. Protective antibodies levels were found in 50/54 (92%) patients and in 67/70 (96%) controls. Moreover, anti-HBs levels were similar in diabetic patients and controls (means of log-titre and (sd); 1.95 (0.88) and 2.18 (0.64) patients and controls, respectively; P=0.11). No cases of clinical hepatitis were reported and all patients and controls remained HBc negative. These data demonstrate the persistence of anti-HBs levels in children, adolescents and young patients with type 1 diabetes after recombinant hepatitis B vaccine showing evidence of longterm immunogenity and protective effect.

  2. Efficacy of prophylactic antiviral therapy and outcomes in HBsAg-negative, anti-HBc-positive patients receiving chemotherapy: a real-life experience.

    Science.gov (United States)

    Papadopoulos, Nikolaos; Deutsch, Melanie; Manolakopoulos, Spilios; Bitsi, Chrysoula; Michalakeas, Helias; Poulakidas, Helias; Tsironi, Eftychia; Giannouli, Stavroula; Papatheodoridis, George V; Koskinas, John; Pectasides, Dimitrios

    2017-01-01

    The aim of this study is to evaluate the outcomes of hepatitis B surface antigen (HBsAg)-negative, anti-HBc-positive patients who received immunosuppressive therapies. We retrospectively evaluated the medical records of HBsAg-negative, anti-HBc-positive patients with hematological diseases or solid tumors who underwent immunosuppressive therapies and were referred because of positive baseline hepatitis B virus (HBV) serology or HBV reactivation. The referral date was according to the judgment of the treating physician at the time of identification of any signs of HBV infection. We included 55 HBsAg-negative, anti-HBc-positive patients. Of these, 31 received antiviral prophylaxis (group 1), whereas 24 patients did not receive any anti-HBV agent (group 2). The majority of patients [49/55 (89%)] had hematological malignancies and most of them 39/55 (71%) received rituximab-containing regimens. Lamivudine was used as antiviral prophylaxis in 13/31 (42%) patients of group 1. One patient in this group experienced HBV reactivation and was treated successfully with tenofovir add-on therapy. All patients in the second group experienced HBV reactivation and most of them [19/24 (79%)] were treated with tenofovir or entecavir as rescue therapy. Two of these patients (one of the tenofovir/entecavir subgroup and one of the lamivudine subgroup) eventually died because of hepatic failure despite rescue treatment. Patients with serological markers of previous HBV infection are still at risk for HBV reactivation. Screening of both anti-HBs and anti-HBc is mandatory before chemotherapy. Pre-emptive antiviral prophylaxis, including lamivudine, is highly effective in all subgroups of such patients, whereas deferring treatment upon HBV reactivation is not enough to rescue all cases.

  3. Patrón de immunoblotting y niveles de anticuerpos anti-Toxoplasma gondii en suero y humor acuoso de pacientes con lesiones de toxoplasmosis ocular

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    Morella Bouchard Pereira

    2014-07-01

    Full Text Available El objetivo de este estudio fue evaluar en muestras de suero y humor acuoso los niveles de anticuerpos anti-toxoplasma a través del Coeficiente de Goldmann y Witmer (CGW y el patrón de reconocimiento antigénico a través del immunoblotting (IB, en pacientes con serología positiva con y sin lesiones de toxoplasmosis ocular. Se recogieron simultáneamente muestras de suero y humor acuoso de 26 pacientes: un grupo de casos que poseían lesiones retinales de toxoplasmosis ocular en fase activa e inactiva (n=17 y un grupo control que requería cirugía ocular por presencia de cataratas (n=9. Las determinación de IgM e IgG específicas se realizó por ELISA de inmunocaptura e indirecto, respectivamente. Se utilizó la inmunodifusión radial para la cuantificación de la IgG total. El CGW resultó >2, indicativo de producción local de anticuerpos específicos en 12/17 de los casos, mientras que en los controles no se observó, esto evidenció una sensibilidad del 71% y una especificidad de 100%. En IB, la aparición de bandas diferentes en humor acuoso, indicativo de producción local de anticuerpos específicos se observaron en 11/17 de los casos y 1/9 de los controles, reflejando una sensibilidad de 65% y una especificidad de 89%. Al considerar las dos pruebas la sensibilidad se incrementó a un 73%, pero la especificidad disminuyó a 89%. En conclusión el IB es útil como prueba confirmatoria para diagnóstico de toxoplasmosis ocular, pero sólo como un complemento del coeficiente de GW especialmente en pacientes con lesiones atípicas donde el diagnóstico clínico es difícil. Aqueous humor and serum immunoblotting profiles and anti–toxoplasma gondii antibodies in patients with toxoplasmosis-induced retinal lesions Abstract The purpose of this study was to analize the anti-Toxoplasma gondii antibodies levels in serum and aqueous humor samples in patients with ocular toxoplasmosis by using Goldman and Witmer Coefficient (GWC and the

  4. Prevalencia de anticuerpos anti-hepatitis C en pacientes en hemodiálisis crónica.

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    Raúl DE LOS RIOS

    1997-04-01

    Full Text Available Objetivo: Determinar la prevalencia de anticuerpos antihepatitis C (Ac HCV en pacientes en hemodiálisis crónica, su correlación con marcadores de enfermedad hepática y los factores de riesgo asociados a la infección. Material y métodos: Se realizó un estudio trasversal y multicéntrico. La población estuvo compuesta por pacientes con insuficiencia renal crónica (IRC, en programa de hemodiálisis (HD crónica intermitente, que acudieron a las sesiones de HD durante los meses de marzo y abril de 1996, en 3 centros de diálisis de Lima. Para determinar Ac HCV se utilizó la prueba de ELISA de segunda generación, asimismo se determinó alanino aminotrasferasa (ALT y fosfatasa alcalina (FA en sangre. Resultados: La población estuvo compuesta por 124 pacientes, 72(58.1% fueron varones (41.9%, mujeres. La edad promedio fue de 54.98 ± 14.3 años. La prevalencia de Ac HCV fue 83.9 ± 6.5 (IC 95%, no encontrándose diferencia entre los 3 centros de diálisis. El único factor de riesgo asociado a la presencia de Ac HCV (+ fue el tiempo de ALT y FA ni de la causa de la IRC, con la presencia de Ac HCV. Conclusión: La prevalencia de Ac HCV es alta en pacientes en hemodiálisis crónica y el factor de riesgo asociado a la infección, es el tiempo en programa de hemodiálisis crónica. (Rev Med Hered 1997; 8: 67-71.

  5. Neuromielitis óptica con alta expresión de acuaporina-4 y anticuerpos anti-acuaporina-4 positivos en suero

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    Alejandra Báez

    2012-04-01

    Full Text Available La presencia de anticuerpos IgG en suero, con blanco en los canales de acuaporina-4, es específica de la neuromielitis óptica (NMO. El 60% de los pacientes con NMO presentan lesiones cerebrales en la resonancia magnética (RM; en un 8% (mayoría niños estas lesiones se consideraron "atípicas". Presentamos dos pacientes con NMO y lesiones en el SNC de alta expresión de acuaporina-4. Caso 1: varón de 50 años, que comenzó con pérdida de visión en ojo derecho (OD. Recibió tratamiento empírico con metilprednisolona 1 g/d x 3 días. Al mes presentó dolor generalizado y hemiparesia derecha; nuevamente recibió metilprednisolona 1 g/d x 5 días e IgG IV 400 mg/kg/d × 5 días. Recuperó la deambulación persistiendo el dolor y fenómenos paroxísticos en los 4 miembros. Potenciales evocados visuales: P100, ojo izquierdo (OI 123 mseg. OD sin respuesta. La RM de cerebro (FLAIR mostró hiperintensidad en nervio óptico derecho, hipotálamo y comisura blanca anterior. RM cervical: lesión medular extensa (5 cuerpos vertebrales. Caso 2: mujer de 53 años, con disminución de la agudeza visual en ambos ojos y parestesias en miembros inferiores que remitieron espontáneamente. Evolucionó al mes con cuadriparesia e incontinencia esfinteriana. Recibió metilprednisolona 1 g/d x 5 días, sin mejoría. Potenciales evocados visuales: P100 OI 124 mseg. OD 128 mseg. RM cerebro: (FLAIR hiperintensidad hipotalámica y periacueductal. RM cervical: lesión medular extensa (7 cuerpos vertebrales. Anticuerpos anti-acuaporina-4 positivos en ambos pacientes (inmunofluorescencia indirecta. Las lesiones consideradas "atípicas", como aquí, en sitios con alta densidad de proteínas canales de agua AQP4 deberán considerarse para el diagnóstico diferencial.

  6. The effects of booster vaccination on combined hepatitis A and hepatitis B vaccine in both anti-HBs and anti-HAV negative children 5-15 years after hepatitis B vaccine primary immunization.

    Science.gov (United States)

    Chen, Yongdi; Gu, Hua; Cheng, Suyun; Shen, Lingzhi; Cui, Fujiang; Wang, Fuzhen; Yao, Jun; Xia, Shichang; Lv, Huakun; Liang, Xiaofeng

    2013-04-01

    In the present study, we investigated the changes in both anti-HAV lgG and anti-HBs lgG levels and compared the antibody seroconversion rates of different doses of combined hepatitis A and hepatitis B vaccine in children. Children who were vaccinated as infants with Hepatitis B vaccine were revaccinated at 5-15 y of age, then the antibody titers were monitored. Among 283 children, this study found that the anti-HAV seroconversion rates (defined as anti-HAV ≥ 1 mIU/ml) after the first and the third dose were 79.9% and 100% respectively; these observed differences were statistically significant (Panti-HBs seroconversion rates (defined as an anti-HBs ≥ 10 mIU/ml) were 82.3% and 99.0% respectively; these observed differences were statistically significant (Panti-HAV seroconversion rate was statistically significant in children aged 5-9 y and 10-15 y (Panti-HBs seroconversion, whereas after the third dose the difference was not statistically significant (P>0.05). This study demonstrated that the immunization effects of booster vaccination with combined hepatitis A and hepatitis B vaccine is successful for children. A single booster dose is adequate for younger children, while three doses are needed for older children.

  7. Detección de anticuerpos anti-Brucella spp. en cerdos mediante técnicas de aglutinación y ELISA indirecto en las provincias de Buenos Aires y La Pampa: Argentina Detection of anti-Brucella spp. antibodies in swine by agglutination techniques and indirect ELISA in the Buenos Aires and La Pampa provinces: Argentina

    OpenAIRE

    H.A. Castro; S.R. González; M.I. Prat; P.C. Baldi

    2006-01-01

    En nuestro país no existe un programa de control sobre brucelosis porcina y su verdadera situación epidemiológica es desconocida. El objetivo de nuestro trabajo fue detectar la presencia de anticuerpos anti-Brucella spp. en porcinos provenientes de criaderos del sudoeste de la provincia de Buenos Aires y del este de la provincia de La Pampa. La toma de muestras de sangre se realizó en el momento del faenado de los animales. La detección de anticuerpos se efectuó mediante las técnicas de aglut...

  8. The combination of anti-HBc and anti-HBs levels is a useful predictor of the development of chemotherapy-induced reactivation in lymphoma patients with resolved HBV infection

    Science.gov (United States)

    Matsubara, Tokuhiro; Nishida, Tsutomu; Shimoda, Akiyoshi; Shimakoshi, Hiromi; Amano, Takahiro; Sugimoto, Aya; Takahashi, Kei; Mukai, Kaori; Yamamoto, Masashi; Hayashi, Shiro; Nakajima, Sachiko; Fukui, Koji; Inada, Masami

    2017-01-01

    Fatal chemotherapy-induced hepatitis B virus reactivation (HBV-R) is a well-described serious complication observed in patients with lymphoma and resolved HBV infection. The aim of the present study was to determine the predictive factors of the development of chemotherapy-induced HBV-R. A total of 77 consecutive newly diagnosed patients with lymphoma and resolved HBV infection, who received chemotherapy from 2007 through 2015 were analysed retrospectively. Significant predictive factors associated with HBV-R were identified based on the data from these patients. Ten patients developed HBV-R during and following chemotherapy, and two of these 10 patients developed HBV-associated hepatitis flares. There was a significant negative correlation between anti-hepatitis B core (HBc) titres prior to chemotherapy and time to HBV-R (P=0.016, R=−0.732). Univariate and multivariate logistic regression analyses demonstrated that anti-HBc and anti-hepatitis B surface (HBs) titres at baseline were significant predictive factors for HBV-R. In addition, patients with high anti-HBc titres at baseline (above 10 S/CO) were significantly more likely to experience HBV-R than patients with low anti-HBc and high anti-HBs titres (above 28 mIU/ml), who did not experience complete reactivation (PHBV-R than those with high anti-HBs titres (P=0.031). All HBV-R episodes among the patients with high anti-HBc titres occurred within 3 months following the initiation of chemotherapy. The combination of anti-HBc and anti-HBs titres, as opposed to either titre alone, at baseline in patients with lymphoma may serve as a surrogate marker for the occurrence of HBV-R under the influence of chemotherapy. PMID:29151907

  9. Seroprevalence of hepatitis B virus infection and seroconvertion to anti-HBsAg in laboratory staff in Goiânia, Goiás Soroprevalência da infecção pelo vírus da hepatite B e soroconversão para anti-HBsAg em profissionais de laboratório em Goiânia, Goiás

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    Paula Andréia Silva

    2005-04-01

    Full Text Available Were analyzed 648 serum samples from laboratory staff in Goiânia, Goiás aiming detection of three serological markers of HBV: HBsAg, anti-HBsAg and anti-HBcAg. The HBsAg and anti-HBcAg positive samples were also analyzed for HBeAg, anti-HBeAg and anti-HBcAgIgM markers. HBV infection rate of 24.1% was observed and, from them, 0.7% were positive for HBsAg. Viral DNA was detected by PCR in two HBsAg positive samples. A vaccination index of 74.5% and a global index of 89.9% of serological response to vaccination were observed. The direct work with biological fluids as well as cleaning workers represented significant risks for acquisition of HBV infection. The data from the present study showed an increase of the vaccination index among laboratory staff but the rates of HBV infection did not change through the years in the region.Neste estudo, foram analisadas 648 amostras de soro, provenientes de profissionais de laboratório de Goiânia-Goiás, visando a detecção de três marcadores sorológicos do VHB: HBsAg, anti-HBsAg e anti-HBcAg. As amostras HBsAg e anti-HBcAg positivas foram também analisadas para os marcadores HBeAg, anti-HBeAg e anti-HBcAgIgM. Foi observado um percentual de soropositividade para infecção pelo VHB de 24,1% sendo que 0,7% destes foram positivos para o HBsAg. O DNA viral foi detectado por PCR nas duas amostras HBsAg positivas. Dos 648 profissionais, 74,5% relataram vacinação para o VHB e, destes, 89,9% apresentaram soroconversão. Trabalho direto com fluidos biológicos bem como trabalho em serviços gerais representaram risco significativo para aquisição da infecção pelo VHB. Os resultados do estudo mostram que embora tenha havido um aumento no índice de vacinação entre os trabalhadores de laboratório, o índice de infecção pelo VHB manteve-se inalterado em relação ao tempo na região.

  10. The effects of different dosage levels of hepatitis B vaccine as booster on anti-HBs-negative children 5-15 y after primary immunization; China, 2009-2010.

    Science.gov (United States)

    Chen, Yongdi; Lv, Huakun; Gu, Hua; Cui, Fujiang; Wang, Fuzhen; Yao, Jun; Xia, Shichang; Liang, Xiaofeng

    2014-01-01

    The changes in lgG antibody levels to hepatitis B surface antigen (HBsAg) and in antibody to HBsAg (anti-HBs) seroconversion rates due to different dosages of hepatitis B vaccine (HepB) were compared in 2106 children. Children who had been previously vaccinated as infants with HepB were revaccinated at 5-15 y of age, after which the antibody titers were determined. After the first booster dose, the anti-HBs seroconversion rate (defined as an anti-HBs ≥10 mIU/ml) with 10 μg of HepB (93.6%) was significantly greater than the rate with 5 µg of HepB (90.3%) (Panti-HBs seroconversion rate in 10-15-y-old boys vaccinated with 10 μg of HepB (90.9%) was significantly greater than the rate with 5 µg of HepB (84.3%) (Panti-HBs seroconversion rates after the third booster dose with 5 or 10 μg of HepB were greater than those after the first booster dose (99.6% and 99.7%, vs. 90.3% and 93.6%, Peffects of booster vaccination with 3 doses of HepB with 5 or 10 µg are effective; a single booster dose with 10 µg of HepB for 10-15-y-old boys and with 5 or 10 µg of HepB for 5-9 y old boys and for 5-15-y-old girls are effective in generating protective antibody against HBV; however, for anti-HBs-negative children after a single dose of booster, 3 doses are needed.

  11. Infección oculta por el virus de la hepatitis B en hijos de madres positivas al HBsAg

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    Marité Bello-Corredor

    2016-04-01

    Full Text Available La infección oculta por el virus de la hepatitis B (IOB, se caracteriza por la presencia en suero o plasma del genoma viral (ADN-VHB y anticuerpos contra la proteína de la cápside (anti-HBc en ausencia del antígeno de superficie (HBsAg, marcador que tradicionalmente se emplea para identificar la presencia del virus. Con el objetivo de caracterizar la presencia de IOB en hijos de madres positivas al HBsAg, se estudiaron 291 muestras séricas de niños con la condición de ser HBsAg (- y anticuerpos anti-HBsAg (anti-HBs menores de 50 UI/L, conservadas en la seroteca del Laboratorio de Referencia Nacional de Hepatitis Virales. Se realizaron ensayos para determinar la exposición al virus (anti-HBc, a los sueros anti-HBc (+ se les realizó Reacción en Cadena de la Polimerasa en Tiempo Real (RCP-TR para determinar y cuantificar el ADN-VHB. La prevalencia de exposición al VHB (anti-HBc fue 16,8% (49/291. El ADN viral se cuantificó en el 14% (6/43 de los casos anti-HBc (+, observándose cargas virales que oscilaban entre 2,15 x 101 hasta 3,42 x 101 UI/mL. La prevalencia de la IOB para el total de los pacientes analizados fue 2,1% (6/291, considerada relativamente baja. No se encontró asociación significativa entre las variables sociodemográficas analizadas tales como: edad, sexo y provincia de procedencia. La IOB está presente en hijos de madres positivas al HBsAg, a pesar de la profilaxis contra la hepatitis B. Por lo tanto, se requiere de pesquisajes adecuados para detectar dicha entidad. Las implicaciones clínicas y epidemiológicas de la misma, requieren de un estrecho monitoreo y atención de estos pacientes. Este estudio se realiza por primera vez en Cuba y aporta conocimientos útiles para el diagnóstico, prevención y control de esta enfermedad en niños.

  12. Original paper Assessment of the level of vaccine-induced anti-HBs antibodies in children with inflammatory systemic connective tissue diseases treated with immunosuppression

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    Izabela Szczygielska

    2015-05-01

    Full Text Available Objectives : Protective vaccinations are the most effective method of prevention of type B virus hepatitis. The aim of the study was to determine whether in children receiving immunosuppressive therapy due to inflammatory systemic connective tissue diseases the protective concentration of the anti-HBs antibodies produced after vaccination against type B virus hepatitis in infancy is maintained. Material and methods : The concentration of anti-HBs antibodies was assessed in the sera of 50 children with inflammatory connective tissue diseases – 37 girls (74% and 13 boys (26%, aged 1.5–17.5 years – during the immunosuppressive treatment, which lasted at least 6 months. The control group consisted of 50 healthy children – 28 girls (56% and 22 boys (44% aged 2–17 years. All children were vaccinated in infancy with Engerix B vaccine according to the 0–1–6 months schedule. The antibody concentration of ≥ 10 mIU/ml in patients is regarded as protective. Results: No protective antibody concentrations were found in 25 cases (50% in the group of diseased children and only in 2 children in the control group (4%. Conclusions : The concentration of vaccine-induced antibodies should be assessed in children with inflammatory systemic connective tissue diseases and, in case of the absence of a protective concentration, revaccination should be started. The use of glucocorticosteroids, synthetic and biological disease-modifying antirheumatic drugs is no contraindication to vaccination against hepatitis B.

  13. Nuevos Conceptos en el Síndrome por Anticuerpos Antisintetasa

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    Labirua-Iturburu Ruiz, Ane

    2013-01-01

    El síndrome por anticuerpos antisintetasa (SAS) es una entidad autoinmune que comprende la presencia de un anticuerpo antisintetasa (AAS), principalmente anti-Jo-1, anti-PL-7 y anti-PL-12 y al menos una de las siguientes características clínicas: miositis, enfermedad pulmonar intersticial –EPI-, artritis, fiebre, fenómeno de Raynaud y “manos de mecánico”. La rareza del síndrome y los pocos estudios al respecto hacen que aún existan múltiples interrogantes en torno a la etiopatogenia, tratamie...

  14. Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance Caracterização de uma cepa de hepatite por vírus B no sudoeste do Paraná, Brasil, apresentando mutações previamente associadas à resistência anti-HBs

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    Dennis Armando Bertolini

    2010-02-01

    Full Text Available The present study investigated if hepatitis B virus (HBV mutants circulate in the southwestern region of the State of Paraná, Brazil, by analyzing samples from children who received immunoprophylaxis but were born to HBV carrier mothers. Samples from 25 children were screened for HBV serum markers and for HBV DNA by PCR. Only one sample was positive for HBsAg, anti-HBs and HBV DNA, although the child had been vaccinated. Analysis of the S gene sequence of this sample showed the presence of a proline at position 105, a serine at position 114, three threonines at positions 115, 116 and 140, and a glutamine at position 129. The presence of these amino acids, except for serine at position 114, has been related to monoclonal or polyclonal therapy with anti-HBs after liver transplantation, whereas the presence of threonine at position 116 has been described in immunized children from Singapore. This finding demonstrates the possible circulation of HBV strains resistant to hepatitis B immunoprophylaxis in southwestern Paraná, Brazil. The genotype of the sample was identified as genotype D, which is frequently found in the region studied. Since 36% of the children had received incomplete or no immunoprophylaxis, more extensive follow-up of children born to HBsAg-positive mothers is needed.O presente estudo investigou se mutantes do vírus da hepatite B (HBV circulam na região Sudoeste do Estado do Paraná, Brasil, analisando amostras de crianças que receberam a imunoprofilaxia por terem nascido de mães portadoras do HBV. Amostras de 25 crianças foram analisadas para os marcadores sorológicos do HBV e para o DNA-HBV por PCR. Somente uma amostra foi positiva para AgHBs, anti-HBs e DNA-HBV, apesar da criança ter sido vacinada. Análises da seqüência do gene S desta amostra mostrou a presença de uma prolina na posição 105, uma serina na posição 114, três treoninas nas posições 115, 116 e 140, e uma glutamina na posição 129. A presen

  15. Anticuerpos anti-CCP en artritis reumatoidea: relación con características clínicas, citocinas Th1/Th2 y HLA-DRB1.

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    Paula A. Correa

    2004-06-01

    Full Text Available En el presente estudio se examinó la especificidad y la sensibilidad de los anticuerpos antipéptidos citrulinados cíclicos (CCP en pacientes latinoamericanas con artritis reumatoidea (AR, así como su relación con la actividad de la enfermedad, manifestaciones extraarticulares (MEA, síntesis de citocinas (IL-4, IL-10, IL-12, TNF-? e IFN-? y factor reumatoideo (FR IgM e IgA, y con el polimorfismo del HLA-DRB1. Se examinaron 79 pacientes con AR (69 con AR establecida y 10 con AR temprana sin previo tratamiento, 56 pacientes con espondilitis anquilosante (EA, 25 con lupus eritematoso sistémico (LES, 50 con síndrome de Sjögren primario (SSp y diez individuos sanos. De las 69 pacientes con AR establecida, 36 fueron reevaluadas 2 años después. La actividad de la AR se examinó según los criterios del Colegio Americano de Reumatología. Los anticuerpos anti-CCP2, el FR y los niveles de citocinas se determinaron mediante inmunoensayo, y la genotipificación del HLA se llevó a cabo por reacción en cadena de la polimerasa utilizando mezclas de iniciadores específicos. Los anticuerpos anti-CCP se observaron en 96% de los pacientes con AR en la primera evaluación y en 86% en la segunda (p=0,12, sin modificación significativa en los valores (131±58,7 vs. 130,6±67,1 UI. Su sensibilidad y especificidad global fue de 94% y 92%, respectivamente, pero cuando sólo se consideraron los niveles altos (>60 UI fueron de 84% y 95%, respectivamente. La razón de probabilidades (RP positiva fue de 12 y la RP negativa de 0,06. El valor predictivo (VP positivo fue de 87% y el VP negativo de 96%. Los anticuerpos anti-CCP se observaron en 12% de los pacientes con LES y con SSp, en 2% de los de EA y en 10% de los controles sanos. En los pacientes con AR no se asociaron con la actividad de la enfermedad, MEA y alelos del HLA-DRB1. Tampoco se observaron correlaciones significativas entre sus valores y los niveles de citocinas. En conclusión, los anticuerpos

  16. Measurements in international units of antibody to hepatitis B surface antigen(anti-HBs) after immunization with a yeast-derived, subtype adr hepatitis B vaccine are considerably different between chemiluminescent immunoassay (CLIA) and chemiluminescent enzyme immunoassay (CLEIA).

    Science.gov (United States)

    Ogata, Norio

    2006-04-01

    The worldwide consensus of the minimum protective anti-HBs level against HBV infection is 10 mIU/mL on assays standardized by the World Health Organization (WHO) reference preparations. To investigate whether this value could be applied to recipients of yeast-derived recombinant HB vaccine containing the major surface protein of subtype adr (Bimmugen, Astellas Pharmaceutical, Tokyo), we compared anti-HBs measurements between chemiluminescent immunoassay (CLIA) (Architect Ausab, Abbott Japan, Tokyo) and chemiluminescent enzyme immunoassay (CLEIA) (Lumipulse Forte, Fujirebio, Tokyo) in given serum samples obtained from the vaccinees. The vaccine and the two assay methods are currently in a wide use in Japan. The study included 300 medical students who completed a standard vaccination course (0, 1 and 6 months). Serum samples obtained 1 month or 13 months after completing the vaccination were simultaneously tested for anti-HBs by CLIA and CLEIA. In 147 samples with quantifiable values on both CLIA and CLEIA (10 to 1000 mIU/mL) the geometric mean titer on CLEIA (225.0 mIU/mL) was significantly higher than that on CLIA (94.5 mIU/mL) (p < 0.0001). Of 26 subjects with CLIA measurements below 10 mIU/mL, 15 samples (57.7%) showed CLEIA measurements more than 10 mIU/mL. Thus, in the subtype adr-vaccinees CLEIA demonstrated considerably high serum anti-HBs measurements compared to CLIA and discordance in determining critical anti-HBs level of 10 mIU/mL was observed in more than half the samples. This suggests that the minimum HBV-protective anti HBs titer of 10 mIU/mL is difficult to be introduced to Japan where subtype adr-HB vaccines or -HBV infection are prevalent, unless characteristics of assay methods are carefully evaluated.

  17. Toxocara seroprevalence in children from a subtropical city in Argentina Prevalencia de anticuerpos anti Toxocara en niños de una ciudad subtropical de Argentina

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    José Mario ALONSO

    2000-08-01

    Full Text Available Most studies from Argentina have focused on toxocariasis as an environmental problem of big cities, and there are no available data about children infection from small or middle-sized cities. In order to assess the prevalence of anti-Toxocara antibodies in infantile population, 206 children from Resistencia, of both sexes, aged 1-14 years old were studied by Elisa testing with E/S T. canis L2 antigens. Hematological parameters and immunoglobulin levels were determined; five days' stool samples were studied and epidemiological data were obtained by means of a questionnaire to parents. Results showed that 73% of the children had one or more dogs living at home, 57% reported geophagia and 37.9% were positive for Toxocara serology, but there was no significant difference in prevalence neither for boys and girls, nor concerning age. An increased risk of infection was observed in age groups 5-6 and 7-8 for boys, and in age groups 3-4 and 5-6 for girls.La mayoría de los estudios realizados en Argentina, han enfocado a la Toxocariosis como un problema medioambiental propio de las grandes ciudades y no existen datos acerca de esta infección entre los niños de ciudades medianas o pequeñas. A fin de determinar la prevalencia de anticuerpos anti-Toxocara en la población infantil de la ciudad de Resistencia, se estudiaron 206 niños de ambos sexos, con edades comprendidas entre los 1-14 años, mediante el test de Elisa, empleando antígenos E/S de larva L2 de Toxocara canis. También se evaluaron los niveles de inmunoglobulinas séricas y los parámetros hematológicos; se efectuaron análisis coproparasitológico seriados en heces de 5 días y se recolectó información epidemiológica mediante cuestionario a los padres. Los resultados obtenidos señalan que el 73% de los niños tenían uno o mas perros viviendo en la casa, que el 57% tenían antecedentes de geofagia y que el 37.9% tenían serología positiva para Toxocara, aunque no hubo diferencias

  18. Anti-HBs levels among children and adolescents with complete immunization schedule against hepatitis B virus. A cross-sectional study in Blumenau, State of Santa Catarina, Brazil, 2007-2008 Níveis de anti-HBs entre crianças e adolescentes com o esquema completo de imunização contra o vírus da hepatite B. Um estudo transversal em Blumenau, Estado de Santa Catarina, 2007-2008

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    Andréa do Livramento

    2011-08-01

    Full Text Available INTRODUCTION: Vaccination is the main tool for preventing hepatitis B virus (HBV infection; however, following the completion of the vaccination series, the concentrations of anti-HBs can decline over the years and reach levels less than 10mIU/mL. The persistence of protection in these individuals is still unknown. The present study aimed to determine the anti-HBs antibody levels among children and adolescents who had received a complete vaccination course for hepatitis B. METHODS: Antibodies against HBV surface antigen (anti-HBs were tested in 371 individuals aged 10 to 15 years-old. RESULTS: Volunteers who showed undetectable quantities of anti-HBs accounted for 10.2% of the population studied and 39.9% presented antibody titers of less than 10mIU/mL. Anti-HBs > 10mIU/mL were verified in 49.9%. CONCLUSIONS: These results corroborate other studies indicating levels of anti-HBs below 10mIU/mL in vaccinated individuals. Additional studies are required to assess whether this indicates susceptibility to HBV infection and the need and age for booster doses.INTRODUÇÃO: A vacinação é o principal instrumento para prevenir a infecção pelo vírus da hepatite B. Todavia, após a conclusão da série de vacinação, as concentrações de anti-HBs podem diminuir ao longo dos anos e atingir níveis inferiores a 10mUI/mL. A persistência da proteção nestes indivíduos ainda é desconhecida. O presente estudo objetivou determinar os níveis do anticorpo anti-HBs em crianças e adolescentes que receberam o esquema completo de vacinação para a hepatite B. MÉTODOS: O anticorpo para o antígeno de superfície do vírus da hepatite B (anti-HBs foi testado em 371 indivíduos com idade entre 10-15 anos. RESULTADOS: Os voluntários que apresentaram quantidades indetectáveis de anti-HBs corresponderam a 10,2% da população estudada, e 39,9% apresentaram títulos do anticorpo inferiores a 10mUI/mL. Anti-HBs > 10mUI/mL foi verificado em 49,9%. CONCLUS

  19. Prevalencia de anticuerpos anti-Leptospira spp. en personas con exposición laboral en el departamento del Tolima / Prevalence of anti-Leptospira spp. antibodies among people with occupational exposure in Tolima Department

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    Blanca L. Guzmán-Barragán

    2016-05-01

    Full Text Available Resumen Objetivo: estimar la prevalencia de anticuerpos IgM contra Leptospira spp., mediante el Ensayo de Inmunoabsorción Ligado a Enzimas (elisa, en la población de riesgo laboral de 8 municipios del Tolima. Metodología: se obtuvieron muestras de sangre de 261 empleados, las cuales fueron analizadas mediante la técnica de elisa para la detección de anticuerpos IgM anti-Leptospira spp., seguido de mat y serotipificación. Resultado: se estimó una seroprevalencia del 25,29%, con una seroreactividad mayor en trabajadores de plantas de beneficio animal (34,2%, recolección de residuos sólidos (27,1% y trabajadores de acueducto y alcantarillado (14,8%. La actividad en plantas de beneficio animal se identificó como factor de riesgo de Leptospira spp. (OR=1,86. Los serovares identificados fueron L. Bratislava (16, Ballum (5, Tarassovi (3, Hebdomadis (2, Sejroe (2 y Icterhemorragiae (1. El municipio de Libano presento el mayor porcentaje de positividad (36,96%, seguido de Espinal y Guamo con 28,57% cada uno. Discusión: la evaluación del sistema de vigilancia indicó deficiencia en recursos y debilidades de los profesionales de la salud al desconocer los procedimientos, investigación, diagnóstico y notificación de la enfermedad. Conclusiones: la leptospirosis está presente en poblaciones de riesgo laboral en el Tolima y se hace necesario abordar esta problemática en la población de otros municipios y los animales transmisores de la enfermedad. / Abstract Objective: to estimate the prevalence of IgM antibodies against Leptospira spp. Using the enzyme-linked immunosorbent assay (elisa in a population at occupational risk from 8 municipalities of the Tolima department, Colombia. Methodology: blood samples were collected from 261 employees and analyzed with the elisa technique to detect IgM and anti-Leptospira spp. antibodies. This was followed by mat and serotyping. Results: a seroprevalence of 25.29% was estimated, with higher

  20. No response to hepatitis B vaccine in infants born to HBsAg(+) mothers is associated to the transplacental transfer of HBsAg.

    Science.gov (United States)

    Wang, Jing; He, Yingli; Jin, Dongfang; Liu, Jinfeng; Zheng, Jie; Yuan, Ningxia; Bai, Yun; Yan, Taotao; Yang, Yuan; Liu, Yong; Zhang, Shulin; Zhao, Yingren; Chen, Tianyan

    2017-08-01

    No or low hepatitis B (HB) vaccine response is more frequent in infants from HBsAg(+) mothers than those from HBsAg(-). Our previous study found temporary positivity of HBsAg in infants from HBsAg(+) mothers. In this study, we hypothesized that HBsAg in infant blunt immune response to standard hepatitis B vaccination. A total of 328 consecutive HBsAg(+) mothers and their offspring were enrolled. Blood samples were taken from mothers and their infants and quantified for HBsAg, anti-HBs titer and HBV DNA load concentration; Placenta samples were collected to stain for HBsAg. First, 6.7% infants (22/328) showed anti-HBs titer lower than 10 mIU/mL after HB vaccination (non-response to HB vaccine). HBsAg(+) newborns showed higher risk of non-response than HBsAg(-) infants (13.0% versus 5.0%, p = 0.016). Infants from high HBsAg titer mothers displayed higher risk of HBsAg positivity at birth than those from low titer mothers (45.3% versus 2.8%, p < 0.001). HBsAg titer in mothers of HBsAg(+) newborns was much higher than mothers of HBsAg(-) newborns (p < 0.001). All those data supported HBsAg can be transferred through placenta. Our hypothesis was further reinforced by immunostaining with specific antibody against HBsAg, a substantial higher prevalence (87.5% versus 30.8%, p = 0.024) and stronger immunostaining (p = 0.008) was demonstrated in HBsAg(+) group comparing with placenta of the HBsAg(-) group. No response to HB vaccine in infants of HBsAg(+) mothers was associated to the transplacental transfer of HBsAg.

  1. Hepatitis B, C and D virus genotypes detected in HBsAg- or anti-HCV-positive people from the Republic of Moldova.

    Science.gov (United States)

    Gori, Ala; Gheorgita, Stela; Spinu, Constantin; Pinzaru, Iurie; Halacu, Ala; Sajen, Octavian; Suveica, Luminița; Sausy, Aurélie; Muller, Claude P; Hübschen, Judith M

    2018-02-01

    In the Republic of Moldova, little is known about hepatitis B, C and D virus (HBV, HCV, HDV) genotypes, although the genetic variant may influence the course and outcome of disease. For HBV genotyping, 301 hepatitis B surface antigen (HBsAg)-positive sera collected in 2010 and 2011 from drug users, prison inmates, commercial sex workers, and the general population in different geographical regions were investigated. The 31 HBsAg-positive sera collected in 2011 were also tested for HDV. Eighty-eight anti-HCV-positive sera collected between 2010 and 2011 from the general population and health care workers were used for HCV genotyping. Phylogenetic analysis of 84 HBV sequences showed that most of the viruses belonged to genotype D (n = 82, 97.6%), predominantly to the subgenotype D1/D2 cluster (n = 75/82, 91.5%). One sequence (74110) clustered as an outlier to this cluster, and six sequences belonged to subgenotype D3. Only two subgenotype A2 sequences were found. Cloning of six samples with ambiguous sequence chromatogram signals showed no mixed infections. Phylogenetic analysis of HCV sequences from 66 patients showed a predominance of subtype 1b (n = 63, 95.5%). Two sequences belonged to subtype 3a, and one to subtype 2a. HDV RNA belonging to genotype 1 was found in two sera (2/31, 6.5%). Thus, genotypes prevalent in Europe were detected for all three hepatitis viruses. For both HBV and HCV, one genotype was dominant, while occasional other variants seem to be restricted to certain cohorts and/or transmission routes.

  2. Anti-HBc testing for blood donations in areas with intermediate hepatitis B endemicity

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    Celina Maria Turchi Martelli

    1999-07-01

    Full Text Available En otros estudios se ha evaluado el menor riesgo de infecciones transmitidas por transfusión sanguínea que acarrea el tamizaje de anticuerpos contra los antígenos nucleares de la hepatitis B (anti-HBc, sumado a la prueba detectora de antígenos de superficie (HbsAg. No obstante, la mayoría de estos estudios se basaron en datos procedentes de zonas con baja endemicidad por hepatitis B o de bancos de sangre de países desarrollados, donde la mayoría de la sangre proviene de personas que han donado previamente. A fin de examinar los pros y los contras del tamizaje de anticuerpos anti-HBc en el Brasil, los autores describen los antecedentes y la interpretación de las pruebas de tamizaje para prevenir la hepatitis postransfusión. También presentan los datos sobre el tamizaje de la hepatitis B en todas las regiones del Brasil. No hay pruebas suficientes para suspender el tamizaje de los anticuerpos anti-HBc en el país. Debe darse alta prioridad a comparar el costo-efectividad de un marcador basado en la biología molecular con el de la actual prueba detectora de anticuerpos anti-HBc aplicada comúnmente, a fin de mejorar las medidas de tamizaje que garantizan la inocuidad de la sangre.

  3. Distribución heterogénea de la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre en Puebla, México Heterogeneous distribution of the prevalence of anti-Trypanosoma cruzi antibodies among blood donors in the State of Puebla, Mexico

    OpenAIRE

    Víctor M Monteón; Pedro A Reyes-López; Adalid Sosa-Palacio; Gloria León-Tello; Jaime Martínez-Murguía; Francisca Sosa-Jurado

    2005-01-01

    OBJETIVO: Identificar la seroprevalencia de anticuerpos anti Trypanosoma cruzi (Ac anti-T. cruzi) en donadores de sangre que habitan en ámbito rural y suburbano, así como las regiones del estado de mayor riesgo y factores asociados. MATERIAL Y MÉTODOS: Estudio transversal realizado de enero a diciembre de 2003. Se analizaron 2 489 donadores de sangre reclutados en 10 puestos de sangrado del Instituto Mexicano del Seguro Social (IMSS) distribuidos en las siete regiones económicas del estado de...

  4. Rabbit IgG antibodies against Phospholipase A2 from Crotalus durissus terrificus neutralize the lethal activity of the venom Los anticuerpos IgG de conejos anti-fosfolipasa A2 de Crotalus durissus terrificus neutralizan la actividad letal del veneno

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    Juan P. Rodríguez

    2006-12-01

    Full Text Available Crotalus durissus terrificus (C.d.t. (South American rattlesnake venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2 and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutralize the lethality of the whole venom. PLA2 was isolated by gel filtration chromatography (Sephadex G-75. Specific antibodies were obtained by subcutaneous and intramuscular inoculation of PLA2 (700 µg with Freund adjuvant. Groups of six mice (20 + 2 g were inoculated with 0.5 ml i.p. of C. d. t. venom (4 µg or a mixture of venom that had been preincubated with the desired volume of IgG antibodies. Mortality, recorded 24 and 48 h after inoculation, showed that IgG anti-PLA2 were more effective than anticrotalic serum in neutralizing the lethal activity. These results demonstrate that it could be possible to obtain an anti-venom made by specific antibodies with a high level of protection against the lethal component of C.d.t. venom, and/or the inclusion of these antibodies as a supplement in heterologous anti-venoms.El veneno de Crotalus durissus terrificus (C.d.t. (Cascabel de Sud América posee actividad miotóxica y neurotóxica, actividades que también exhibe el complejo crotoxina, principal componente tóxico de este veneno. El complejo crotoxina está constituido por una fosfolipasa A2 básica (PLA2 y una proteína acídica no tóxica, el crotapotín. En este trabajo se estudió la capacidad neutralizante de anticuerpos IgG anti-PLA2 sobre la letalidad inducida por el veneno entero. El antígeno PLA2, fue aislado por cromatografía de filtración en gel (Sephadex G-75. Se inocularon conejos machos por vía subcutánea e intramuscular, con 700 µg de PLA2 y adyuvante para la obtención de anticuerpos específicos. La capacidad neutralizante del

  5. Trasplante renal en pacientes con anticuerpos dirigidos contra donante

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    Federico Cicora

    2014-10-01

    Full Text Available En trasplante renal, los anticuerpos donante-específicos por ensayos de fase sólida predicen el rechazo temprano mediado por anticuerpos, incluso con resultados negativos de citometría de flujo o citotoxicidad dependiente del complemento. Aquí se describen los protocolos de inmunosupresión y los resultados a diez meses de cuatro pacientes en los que se detectó anticuerpos donante-específicos anti-antígenos leucocitarios humanos (HLA por Luminex®, pero no detectados por el método de citotoxicidad dependiente de complemento (CDC ni por citometría de flujo. Los cuatro pacientes recibieron tratamiento de inducción con 5 dosis de timoglobulina de 1.25 mg/kg y 5 dosis de inmunoglobulina intravenosa (IVIG de 400 mg/kg. Además, uno recibió 20 mg de basiliximab el mismo día del trasplante y el día 4 postrasplante; otro recibió 3 sesiones de plasmaféresis en los días -5, -3, y -1 y eculizumab en dosis de 1200 mg antes del trasplante, 900 mg el día 1, and 600 mg por semana durante un mes. En todos los casos, la inmunosupresión de mantenimiento consistió en tacrolimus, micofenolato y deltisona. Todos presentaron buenos resultados en el corto plazo. Nuestra experiencia sugiere que los pacientes con anticuerpos donante-específicos anti-HLA detectados solo por Luminex® deben recibir un seguimiento estricto y que en esta población se pueden obtener buenos resultados a partir del uso de terapia de inducción con timoglobulina e IVIG.

  6. Anticuerpo anticitrulina y manifestaciones extra articulares en artritis reumatoidea

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    María Jezabel Haye Salinas

    2013-02-01

    Full Text Available Los pacientes con artritis reumatidea (AR pueden desarrollar manifestaciones extra articulares (MExA, relacionadas a su morbi-mortalidad. Los anticuerpos anti-péptidos citrulinados cíclicos (ACCP son específicos para la AR y estan relacionados con el daño articular; y podrían tener rol patogénico en las MExA. Nuestro objetivo fue determinar la relación entre los anticuerpos ACCP y MExA en pacientes con AR. Se incluyeron 74 pacientes con diagnóstico de AR (ACR 1987 mayores de 18 años, de más de 6 meses de evolución, con MExA, y un control apareado por sexo y edad sin MExA por cada paciente. Las variables demográficas, clínicas y de laboratorio se compararon con test t, chi cuadrado o Mann-Whitney. Se realizó análisis multivariado; p ≤ 0.05. Los pacientes con MExA presentaron mayor título de anticuerpo ACCP (116 vs. 34, p < 0.01 y de factor reumatoideo (FR (108 vs. 34.5, p < 0.01. En el análisis multivariado hubo asociación entre la presencia de MExA y tabaquismo activo (p = 0.02, OR: 3.78, IC 95%: 1.17-12.2, FR positivo (p = 0.04, OR: 3.23, IC95%: 1.04-11.8 y anticuerpo ACCP positivo (p = 0.04, OR: 3.23, IC 95%: 1.04-10. Presentaron mayor título de anticuerpo ACCP que los controles los pacientes con xerostomía (109 vs. 34, p = 0.04, xeroftalmia (150 vs. 34, p < 0.01, nódulos sub-cutáneos (NSC (141 vs. 34, p < 0.01 y fibrosis pulmonar (158 vs. 34, p = 0.04. En conclusión, el anticuerpo ACCP positivo, el FR positivo y el tabaquismo activo fueron factores de riesgo independientes para el desarrollo de MExA.

  7. Application of a newly developed high-sensitivity HBsAg chemiluminescent enzyme immunoassay for hepatitis B patients with HBsAg seroclearance.

    Science.gov (United States)

    Shinkai, Noboru; Matsuura, Kentaro; Sugauchi, Fuminaka; Watanabe, Tsunamasa; Murakami, Shuko; Iio, Etsuko; Ogawa, Shintaro; Nojiri, Shunsuke; Joh, Takashi; Tanaka, Yasuhito

    2013-11-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstrated seroclearance by the Abbott Architect assay. The performance of the Lumipulse HBsAg-HQ assay was compared with that of a quantitative HBsAg detection system (Abbott Architect) and the Roche Cobas TaqMan HBV DNA assay (CTM) (lower limit of detection, 2.1 log copies/ml) using blood serum samples from patients who were determined to be HBsAg seronegative by the Abbott Architect assay. Ten patients had spontaneous HBsAg loss. Of 8 patients treated with nucleotide analogues (NAs), two were HBsAg seronegative after stopping lamivudine therapy and 6 were HBsAg seronegative during entecavir therapy. Eight acute hepatitis B (AH) patients became HBsAg seronegative. Of the 26 patients, 16 were HBsAg positive by the Lumipulse HBsAg-HQ assay but negative by the Abbott Architect assay. The differences between the two assays in terms of detectable HBsAg persisted over the long term in the spontaneous loss group (median, 10 months), the NA-treated group (2.5 months), and the AH group (0.5 months). In 9 patients, the Lumipulse HBsAg-HQ assay detected HBsAg when HBV DNA was negative by the CTM assay. HBsAg was also detected by the Lumipulse HBsAg-HQ assay in 4 patients with an anti-HBs concentration of >10 mIU/ml, 3 of whom had no HBsAg escape mutations. The automatic, highly sensitive HBsAg CLEIA Lumipulse HBsAg-HQ is a convenient and precise assay for HBV monitoring.

  8. Auto-anticorpos anti-β2-glicoproteína I e síndrome metabólica Anticuerpos anti-β2-glicoproteína I y síndrome metabólico Anti-beta2-glycoprotein I autoantibodies and metabolic syndrome

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    Rodrigo B. Krás Borges

    2011-04-01

    Full Text Available FUNDAMENTO: A síndrome metabólica (SM é uma entidade pró-aterogênica. Autoanticorpos tais como β2-glicoproteína I (β2-gpI podem influenciar o aparecimento de ateromas. Estudos anteriores confirmaram uma associação entre anticorpos IgA anti-β2-gpI e isquemia cerebral, infarto do miocárdio, doença arterial periférica e doença da carótida. OBJETIVO: O objetivo desse estudo de caso-controle foi avaliar uma possível associação entre anticorpos anti-β2-gpI e anticardiolipina (aCL com SM não-complicada. MÉTODOS: Pacientes com SM sem histórico de eventos vasculares e indivíduos-controle, consistindo em pacientes da Enfermaria de Ortopedia admitidos devido a doenças musculoesqueléticas foram incluídos no estudo. Idade, sexo, etnia, histórico de hipertensão, tabagismo, hipercolesterolemia e diabetes mellitus foram avaliados como fatores de risco em ambos os grupos. Anticorpos IgG, IgM, e IgA anti-β2-gpI e aCL foram detectados através de imunoensaios enzimáticos. RESULTADOS: Um total de 68 pacientes com SM e 82 controles foram estudados. Os pacientes com SM tinham média de idade superior à dos controles (P = 0,001, enquanto homens (P = 0,003; OR 0,31; IC95%: 0,15-0,16 e etnia caucasiana (P = 0,004; OR 0,25; IC95%:0,10-0,60 eram predominantes nos controles. Histórico de hipertensão, hipercolesterolemia e diabetes mellitus foi mais prevalente nos pacientes com SM do que nos controles (P FUNDAMENTO: El síndrome metabólico (SM es una entidad pro-aterogénica. Autoanticuerpos tales como β2-glicoproteína I (β2-GPI pueden influir en la aparición de ateromas. Estudios previos han confirmado una asociación entre anticuerpos IgA anti-β2-GPI y la isquemia cerebral, infarto de miocardio, enfermedad arterial periférica y enfermedad carotidea. OBJETIVO: El objetivo de este estudio de caso-control fue evaluar una posible asociación entre los anticuerpos anti-β2-GPI y anticardiolipina (aCL con SM complicada. MÉTODOS: Se

  9. Marcadores inflamatórios e anticorpos anti-chlamydia em pacientes com síndrome metabólica Marcadores inflamatorios y anticuerpos anti-chlamydia en pacientes con síndrome metabólico Inflammatory markers and antichlamydial antibodies in patients with metabolic syndrome

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    Rosecler Riethmuller Franco

    2011-02-01

    ão mostraram diferença significativa em pacientes com SM, com e sem eventos.FUNDAMENTO: El síndrome metabólico está asociado al aumento de riesgo de eventos cardiovasculares. Marcadores inflamatorios y anticuerpos anti-Chlamydia han sido relacionados al desarrollo y a la progresión de la aterosclerosis y de los eventos cardiovasculares. OBJETIVO: Evaluar los marcadores inflamatorios interleucina-6 (IL-6 y factor de necrosis tumoral-alfa (TNF-α y los anticuerpos anti-Chlamydia pneumoniae en pacientes con síndrome metabólico (SM, con y sin eventos cardiovasculares. MÉTODOS: Estudio transversal constituido por 147 individuos. De estos, 100 (68% con SM y sin eventos cardiovasculares; y 47 (32% con SM y con eventos cardiovasculares. De los individuos que habían sufrido eventos cardiovasculares, 13 (6,11% presentan infarto agudo de miocardio (IAM, y diez (4,7%, accidente cerebro vascular (ACV. El diagnóstico del SM fue determinado por los criterios del NCEP-ATPIII. RESULTADOS: La media de edad de los sujetos con eventos cardiovasculares fue de 61,26 ± 8,5 y de 59,32 ± 9,9 en los individuos sin esos eventos (p=0,279, habiendo predominio del sexo femenino. El grupo con SM y sin evento presentó mayor peso, altura, IMC y circunferencia abdominal. Para los individuos con eventos cardiovasculares (p=0,001, los marcadores inflamatorios IL-6 y TNF-α y la enfermedad vascular periférica fueron significativamente mayores. Se obtuvieron niveles elevados de anticuerpos IgG para Chlamydia pneumoniae en el grupo SM, sin eventos y de IgA en el grupo con eventos cuando fueron comparados los dos grupos. Con relación al IAM y al ACV, los anticuerpos anti-Chlamydia pneumoniae no demostraron significación estadística, comparados al grupo sin eventos cardiovasculares. Asociación fue observada con el uso de estatinas, hipoglicemiantes orales, inyectables y antiinflamatorios no esteroides en el grupo con esos eventos. CONCLUSIÓN: Marcadores inflamatorios se encuentran

  10. Neuromielitis óptica con alta expresión de acuaporina-4 y anticuerpos anti-acuaporina-4 positivos en suero Neuromyelitis optica with high aquaporin-4 expression and positive serum aquaporin-4 autoantibodies

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    Alejandra Báez

    2012-04-01

    Full Text Available La presencia de anticuerpos IgG en suero, con blanco en los canales de acuaporina-4, es específica de la neuromielitis óptica (NMO. El 60% de los pacientes con NMO presentan lesiones cerebrales en la resonancia magnética (RM; en un 8% (mayoría niños estas lesiones se consideraron "atípicas". Presentamos dos pacientes con NMO y lesiones en el SNC de alta expresión de acuaporina-4. Caso 1: varón de 50 años, que comenzó con pérdida de visión en ojo derecho (OD. Recibió tratamiento empírico con metilprednisolona 1 g/d x 3 días. Al mes presentó dolor generalizado y hemiparesia derecha; nuevamente recibió metilprednisolona 1 g/d x 5 días e IgG IV 400 mg/kg/d × 5 días. Recuperó la deambulación persistiendo el dolor y fenómenos paroxísticos en los 4 miembros. Potenciales evocados visuales: P100, ojo izquierdo (OI 123 mseg. OD sin respuesta. La RM de cerebro (FLAIR mostró hiperintensidad en nervio óptico derecho, hipotálamo y comisura blanca anterior. RM cervical: lesión medular extensa (5 cuerpos vertebrales. Caso 2: mujer de 53 años, con disminución de la agudeza visual en ambos ojos y parestesias en miembros inferiores que remitieron espontáneamente. Evolucionó al mes con cuadriparesia e incontinencia esfinteriana. Recibió metilprednisolona 1 g/d x 5 días, sin mejoría. Potenciales evocados visuales: P100 OI 124 mseg. OD 128 mseg. RM cerebro: (FLAIR hiperintensidad hipotalámica y periacueductal. RM cervical: lesión medular extensa (7 cuerpos vertebrales. Anticuerpos anti-acuaporina-4 positivos en ambos pacientes (inmunofluorescencia indirecta. Las lesiones consideradas "atípicas", como aquí, en sitios con alta densidad de proteínas canales de agua AQP4 deberán considerarse para el diagnóstico diferencial.Disease-specific aquaporin-4 antibodies (NMO-IgG are the main effector of lesions in neuromyelitis optica (NMO patients. Brain MRI lesions are detected in 60% of them, with 8% (almost infants at sites of high

  11. ¿Es necesario investigar anticuerpos IgM contra el virus de la hepatitis A cuando el enzimograma hepático es normal? Is it necessary to investigate anti-hepatitis A virus (HAV IgM antibodies when the hepatic enzymogram is normal?

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    C. Coitinho

    2007-09-01

    Full Text Available La hepatitis por virus A (VHA es la más frecuente de las hepatitis virales en el mundo, especialmente en los países subdesarrollados. Donde esta enfermedad es endémica, se suelen realizar un gran número de estudios de laboratorio para confirmarla. El objetivo del presente trabajo fue evaluar la utilidad de investigar anticuerpos IgM anti hepatitis A (IgM anti-VHA para el diagnóstico de la VHA en pacientes con niveles séricos normales de aspartato y alanina aminotransferasas (AST/ALT. Todos los pacientes que concurrieron al laboratorio con solicitud de enzimograma hepático y anticuerpos IgM anti-VHA durante el período octubre 2005-marzo 2006 fueron evaluados en este estudio. Los datos clínicos más frecuentes fueron presunción de hepatitis e ictericia (27,5 y 12,7%. La determinación de IgM anti-VHA se realizó por ensayo inmunoenzimático de micropartículas (MEIA; la de enzimas hepáticas en un multianalizador. De los 158 pacientes estudiados, 84 tenían valores elevados de AST/ALT; dentro de este grupo, el 82% fue reactivo para IgM anti-VHA. Los 74 pacientes restantes mostraron niveles normales de AST/ALT y solo 7 de ellos, bajo control de evolución por VHA ya confirmada, fueron reactivos para IgM anti-VHA. El 49% de los pacientes IgM anti-VHA reactivos correspondió a menores de 10 años. De acuerdo con estas observaciones, sugerimos que los estudios de laboratorio para confirmar infección aguda por VHA deberían realizarse en forma secuencial, ya que es innecesaria la determinación de anticuerpos IgM anti-VHA cuando las aminotransferasas séricas son normales.Type A viral Hepatitis (HAV is the most frequent viral hepatitis around the world, especially in low income countries. In order to confirm this disease, a lot of laboratory tests are annually carried out where HAV is endemic. Our objective was to establish the utility of investigating anti-hepatitis A virus (HAV IgM antibodies for HAV diagnosis in patients with normal

  12. Anticuerpos policlonales contra la proteína recombinante NS3 del virus del dengue

    Directory of Open Access Journals (Sweden)

    Liliana Morales

    2017-01-01

    Resultados. Los anticuerpos producidos fueron útiles en ensayos de Western blot e inmunofluorescencia y se reportó por primera vez un anticuerpo policlonal anti-NS3 que permitió la inmunoprecipitación de la proteína viral y la detecta con Western blot sin necesidad de inducir sobreexpresión de NS3 o de usar extractos de células marcados metabólicamente con radioisótopos. Conclusión. Las proteínas recombinantes expresadas y los anticuerpos producidos constituyen herramientas valiosas para estudiar procesos infecciosos del DENV que involucren a la proteína NS3 y evaluar pruebas dirigidas a interferir las funciones de esta proteína.

  13. Analysis of hepatitis B surface antigen (HBsAg) using high-sensitivity HBsAg assays in hepatitis B virus carriers in whom HBsAg seroclearance was confirmed by conventional assays.

    Science.gov (United States)

    Ozeki, Itaru; Nakajima, Tomoaki; Suii, Hirokazu; Tatsumi, Ryoji; Yamaguchi, Masakatsu; Kimura, Mutsuumi; Arakawa, Tomohiro; Kuwata, Yasuaki; Ohmura, Takumi; Hige, Shuhei; Karino, Yoshiyasu; Toyota, Joji

    2018-02-01

    We investigated the utility of high-sensitivity hepatitis B surface antigen (HBsAg) assays compared with conventional HBsAg assays. Using serum samples from 114 hepatitis B virus (HBV) carriers in whom HBsAg seroclearance was confirmed by conventional HBsAg assays (cut-off value, 0.05 IU/mL), the amount of HBsAg was re-examined by high-sensitivity HBsAg assays (cut-off value, 0.005 IU/mL). Cases negative for HBsAg in both assays were defined as consistent cases, and cases positive for HBsAg in the high-sensitivity HBsAg assay only were defined as discrepant cases. There were 55 (48.2%) discrepant cases, and the range of HBsAg titers determined by high-sensitivity HBsAg assays was 0.005-0.056 IU/mL. Multivariate analysis showed that the presence of nucleos(t)ide analog therapy, liver cirrhosis, and negative anti-HBs contributed to the discrepancies between the two assays. Cumulative anti-HBs positivity rates among discrepant cases were 12.7%, 17.2%, 38.8%, and 43.9% at baseline, 1 year, 3 years, and 5 years, respectively, whereas the corresponding rates among consistent cases were 50.8%, 56.0%, 61.7%, and 68.0%, respectively. Hepatitis B virus DNA negativity rates were 56.4% and 81.4% at baseline, 51.3% and 83.3% at 1 year, and 36.8% and 95.7% at 3 years, among discrepant and consistent cases, respectively. Hepatitis B surface antigen reversion was observed only in discrepant cases. Re-examination by high-sensitivity HBsAg assays revealed that HBsAg was positive in approximately 50% of cases. Cumulative anti-HBs seroconversion rates and HBV-DNA seroclearance rates were lower in these cases, suggesting a population at risk for HBsAg reversion. © 2017 The Japan Society of Hepatology.

  14. Low prevalence of hepatitis B and C among tuberculosis patients in Duhok Province, Kurdistan: Are HBsAg and anti-HCV prerequisite screening parameters in tuberculosis control program?

    Science.gov (United States)

    Merza, Muayad A; Haji, Safer M; Alsharafani, Abid Mohialdeen Hasan; Muhammed, Shivan U

    2016-09-01

    Viral hepatitis, particularly hepatitis B virus (HBV) and hepatitis C virus (HCV), infections and tuberculosis (TB) are a global public health concern. Co-infection with HBV or HCV among TB patients may potentiate the risk of hepatotoxicity induced by anti-TB drugs. Hence, the aim of this study was to identify the prevalence of HBV and HCV among TB patients included in the Duhok National Tuberculosis Program (NTP). The Duhok NTP Center is a specialized institution in Duhok City, Iraq, concerned with management and follow-up of TB patients. A cross-sectional study was conducted at the center between June 2015 and May 2016. All documented TB patients were analyzed on the basis of socio-demographic and other characteristics. Thereafter, all patients underwent screening for hepatitis B surface antigen (HBsAg), anti-HCV, and anti-HIV using enzyme-linked immunosorbent assay (ELISA). The results obtained were analyzed by entering the data in binary format into a Microsoft Excel spreadsheet. A p value of Kurdistan, the negative history of injection drug use, and adherence to universal infection-control measures, including vaccination for HBV. Both history of dental intervention and belonging to a Syrian population were independent risk factors for HBV/TB co-infection. Copyright © 2016 Asian-African Society for Mycobacteriology. Published by Elsevier Ltd. All rights reserved.

  15. Low prevalence of hepatitis B and C among tuberculosis patients in Duhok Province, Kurdistan: Are HBsAg and anti-HCV prerequisite screening parameters in tuberculosis control program?

    Directory of Open Access Journals (Sweden)

    Muayad A Merza

    2016-01-01

    Full Text Available Objective/background: Viral hepatitis, particularly hepatitis B virus (HBV and hepatitis C virus (HCV, infections and tuberculosis (TB are a global public health concern. Co-infection with HBV or HCV among TB patients may potentiate the risk of hepatotoxicity induced by anti-TB drugs. Hence, the aim of this study was to identify the prevalence of HBV and HCV among TB patients included in the Duhok National Tuberculosis Program (NTP. Methods: The Duhok NTP Center is a specialized institution in Duhok City, Iraq, concerned with management and follow-up of TB patients. A cross-sectional study was conducted at the center between June 2015 and May 2016. All documented TB patients were analyzed on the basis of socio-demographic and other characteristics. Thereafter, all patients underwent screening for hepatitis B surface antigen (HBsAg, anti-HCV, and anti-HIV using enzyme-linked immunosorbent assay (ELISA. The results obtained were analyzed by entering the data in binary format into a Microsoft Excel spreadsheet. A p value of <.05 was considered to be statistically significant. Results: Two-hundred fourteen documented TB patients were recruited in this study, with 127 (59.3% males and 87 (40.7% females. The mean age of the patients was 40.34 years (±20.29. Of the total number of patients, four cases (1.8% were HBsAg-positive and one case (0.9% was positive for anti-HCV. The variables significantly associated with HBV were history of surgical dental procedure [odds ratio (OR, 0.04; 95% confidence interval (CI, −0.01 to 0.04; p = .03], and nationality (OR, 13.67; 95% CI, 0.46–210.85; p = .007. Conclusion: The prevalence of HBV and HCV co-infection among TB patients in this study was low. This may be explained by the low rate of blood transfusion among the patients, the very low prevalence of HIV infections in Kurdistan, the negative history of injection drug use, and adherence to universal infection-control measures, including vaccination for HBV

  16. Anticuerpos anti-CCP en artritis reumatoidea: relación con características clínicas, citocinas Th1/Th2 y HLA-DRB1.

    OpenAIRE

    Paula A. Correa; Gabriel J. Tobón; Gustavo Citera; José Cadena; Emilce Schneeberger; José F. Camargo; José A. Maldonado-Cocco; Juan Manuel Anaya

    2004-01-01

    En el presente estudio se examinó la especificidad y la sensibilidad de los anticuerpos antipéptidos citrulinados cíclicos (CCP) en pacientes latinoamericanas con artritis reumatoidea (AR), así como su relación con la actividad de la enfermedad, manifestaciones extraarticulares (MEA), síntesis de citocinas (IL-4, IL-10, IL-12, TNF-? e IFN-?) y factor reumatoideo (FR) IgM e IgA, y con el polimorfismo del HLA-DRB1. Se examinaron 79 pacientes con AR (69 con AR establecida y 10 con AR temprana si...

  17. Low occurrence of HBsAg but high frequency of transient occult HBV infection in vaccinated and HBIG-administered infants born to HBsAg positive mothers.

    Science.gov (United States)

    Zhou, Shan; Li, Tingting; Allain, Jean-Pierre; Zhou, Bin; Zhang, Yuming; Zhong, Mei; Fu, Yongshui; Li, Chengyao

    2017-12-01

    The status of chronic and occult HBV infection (OBI) in neonatal hepatitis B vaccine and immunoglobulin (HBIG) vaccinated infants born to HBsAg+ mothers was investigated at a major hospital in China. Seventy-seven and 15 blood samples were collected in first or second follow-up detection from the vaccinated babies aged 3-36 months born to 43 HBsAg+ or plus 25 HBeAg+ mothers. HBV infection was analyzed between the paired baby and mother by serology and DNA analysis. Among 77 children born to 68 HBsAg+ mothers, 3.9% (3/77) were HBsAg+, and 36.4% (28/77) were HBV DNA+/HBsAg- (OBIs) by a single PCR, respectively. Thirteen of 28 HBV DNA+/HBsAg- samples were conformed by two PCRs or S sequence, which accounted for 16.9% (13/77) of children. Three HBsAg+ and six OBIs were genotyped in consistent with their mother's HBV strains. Of 77 babies' blood samples, anti-HBs reactivity varied slightly according to age groups, while passively transmitted anti-HBc reactivity declined from 100% high reactivity at age 3-5 months to mostly negative at age ≥12 months. Babies with apparent OBI had higher levels of anti-HBc and lower levels of anti-HBs than those without OBI but all eight OBI babies with second follow-up samples became HBV DNA negative beyond 1 year of age. The vaccinated infants born to HBsAg+ mothers presented the low rate of HBsAg occurrence as vaccination failure and high frequency of viral persistence in the form of transient OBIs since no evidence of active HBV infection occurred beyond 1 year of age. © 2017 Wiley Periodicals, Inc.

  18. Detección de anticuerpos anti-Brucella spp. en cerdos mediante técnicas de aglutinación y ELISA indirecto en las provincias de Buenos Aires y La Pampa: Argentina Detection of anti-Brucella spp. antibodies in swine by agglutination techniques and indirect ELISA in the Buenos Aires and La Pampa provinces: Argentina

    Directory of Open Access Journals (Sweden)

    H.A. Castro

    2006-04-01

    Full Text Available En nuestro país no existe un programa de control sobre brucelosis porcina y su verdadera situación epidemiológica es desconocida. El objetivo de nuestro trabajo fue detectar la presencia de anticuerpos anti-Brucella spp. en porcinos provenientes de criaderos del sudoeste de la provincia de Buenos Aires y del este de la provincia de La Pampa. La toma de muestras de sangre se realizó en el momento del faenado de los animales. La detección de anticuerpos se efectuó mediante las técnicas de aglutinación con antígeno tamponado en placa (BPA, seroaglutinación en tubo (SAT, aglutinación con 2-ME (2-ME y ELISA indirecto, con dos antígenos diferentes: el antígeno CYT (fracción citoplasmática de B. abortus S19 y el antígeno CP (extracto citoplasmático libre de lipopolisacárido. Del total de las muestras analizadas (n=325, el 17,8% fue positivo para BPA, el 13,8% fue positivo para SAT y sólo el 8,0% fue positivo para 2-ME. Mediante ELISA-CYT, este porcentaje se elevó a 21,0%, mientras que a través del ELISA-CP sólo se halló un 10,0% de muestras reactivas. Estos resultados son compatibles con los informados en los escasos reportes previos para todo el país y sugieren la necesidad de extender los estudios a otras zonas, donde sea habitual la cría de cerdos.Porcine brucellosis is one of the most important zoonoses in this country. Currently, there is no control program for porcine brucellosis in Argentina and the epidemiological situation is still unknown. The purpose of our study was to detect anti-Brucella spp. antibodies in swine in the southwest of the Buenos Aires province and the east of the La Pampa province. Blood samples were obtained when animals were slaughtered. The presence of anti-brucella antibodies was studied by the buffered plate agglutination test (BPA, the tube agglutination test (SAT, the 2-mercaptoethanol (2-ME agglutination test and indirect ELISA tests, using the cytosolic fraction from Brucella abortus S19

  19. Thyroid autoantibodies in autoimmune diseases Anticuerpos antitiroideos en enfermedades autoinmunes

    Directory of Open Access Journals (Sweden)

    Regina M. Innocencio

    2004-06-01

    Full Text Available Abnormalities in the thyroid function and thyroid autoantibodies have been frequently described in patients with autoimmune diseases but seldom in antiphospholipid syndrome patients. In order to determine the prevalence of thyroid function and autoimmune abnormalities, we compared serum thyrotropin (TSH, serum free thyroxine (T4 levels, thyroid antithyroglobulin (TgAb and antithyroperoxidase (TPOAb levels of 25 patients with systemic sclerosis, 25 patients with rheumatoid arthritis and 13 patients with antiphospholipid syndrome to a control group of 113 healthy individuals. Evaluation included a thorough clinical examination with particular attention to thyroid disease and a serologic immune profile including rheumatoid factor, antinuclear and anticardiolipin antibody measurements. Subclinical hypothyroidism (4.2Ciertas anormalidades en la función tiroidea y anticuerpos antitiroideos han sido frecuentemente descriptos en pacientes con enfermedades autoinmunes, y más raramente en pacientes con el síndrome antifosfolipídico. Para determinar la prevalencía de anormalidades en la función tiroidea y de autoinmunidad, comparamos los niveles séricos de tirotropina (TSH tiroxina libre en suero (T4 anticuerpos antitiroglobulina (TgAb y antitiroperoxidasa (TPOAb en 25 pacientes con esclerosis sistémica, 25 pacientes con artritis reumatoidea y 13 pacientes con el síndrome antifosfolipídico con un grupo control de 113 individuos aparentemente sanos. La evaluación incluyó un completo examen clínico con particular atención para las enfermedades de la tiroides y una evaluación inmunológica incluyendo dosaje del factor reumatoideo, anticuerpos antinucleares y anticardiolipina. Hipotiroidismo subclínico (4.2anticuerpos anti-tiroglobulina (TgAb y/o anti-peroxidasa (TPOAb estaban presentes en 21/63 (33% de los

  20. Prevalencia de anticuerpos antirrubéola y antiparvovirus B19 en embarazadas de la ciudad de Córdoba y en mujeres en edad fértil de la ciudad de Villa Mercedes, San Luis Prevalence of anti-rubella and anti-parvovirus B19 antibodies in pregnant women in the city of Córdoba, and in women of fertile age in the city of Villa Mercedes, province of San Luis

    Directory of Open Access Journals (Sweden)

    M. S. Pedranti

    2007-03-01

    Full Text Available Se determinó la prevalencia de anticuerpos contra virus rubéola en 100 muestras de suero de mujeres embarazadas que concurrían a chequeos de rutina en una institución privada de la ciudad de Córdoba y en 100 muestras de suero de mujeres en edad fértil (42 de ellas embarazadas que concurrían a dispensarios de la ciudad de Villa Mercedes, provincia de San Luis. En las muestras tomadas en la ciudad de Córdoba también se determinaron anticuerpos IgG contra parvovirus B19. Por inhibición de la hemoaglutinación, los resultados de los sueros de Córdoba mostraron una prevalencia de anticuerpos antirrubéola del 98%; en las muestras de Villa Mercedes se observó una prevalencia del 96%. La prevalencia de anticuerpos antiparvovirus B19 en los sueros de Córdoba fue del 66%. Estos datos se asemejan a los de la bibliografía mundial y fundamentan el interés en continuar estudios de este tipo para monitorear el plan de inmunización para rubéola, que en Argentina se lleva a cabo desde 1997, como así también la relevancia de la determinación de IgM antiparvovirus B19 en aquellas embarazadas sintomáticas con resultado negativo para rubéola, a fin de elaborar un diagnóstico diferencial.We determined the prevalence of anti-rubella antibodies in 100 serum samples from pregnant women who attended routine examination at a private institution in the city of Córdoba, and in 100 serum samples from women of gestational age, 42 of whom were pregnant, attending health centres in the city of Villa Mercedes, province of San Luis. IgG antibodies against parvovirus B19 were also determined in the serum samples from Córdoba. Using the hemmagglutination inhibition test, we found a 98 % prevalence of anti-rubella antibodies among pregnant women in Córdoba and of 96 % among the women in Villa Mercedes, whereas the prevalence of anti-parvovirus B19 was 66% in the serum samples from Córdoba. These results coincide with those reported for other cities in the

  1. Prevalencia de anticuerpos anti-Chlamydia trachomatis y anti-Neisseria gonorrhoeae en grupos de individuos de la población mexicana Prevalence of antibodies against Chlamydia trachomatis and Neisseria gonorrhoeae in Mexican populations

    Directory of Open Access Journals (Sweden)

    María del Carmen Cravioto

    2003-01-01

    Full Text Available OBJETIVO: Estimar la prevalencia de infección por Chlamydia trachomatis (CT y Neisseria gonorrhoeae (NG en grupos de individuos con diferente riesgo para infecciones de transmisión sexual. MATERIAL Y MÉTODOS: Estudio transversal multicéntrico efectuado en el Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, de la Ciudad de México, de enero de 1992 a diciembre de 1993, en el que se estudiaron 945 individuos en edad reproductiva, 585 mujeres y 360 hombres. Según su riesgo para infecciones de transmisión sexual se clasificaron en grupos de alto y bajo riesgo. Los de alto riesgo incluyeron mujeres infértiles con daño tubario, mujeres con embarazo ectópico o aborto, hombres infértiles, pacientes con VIH/SIDA, hombres homo o bisexuales y trabajadoras del sexo comercial (TSC. Los de bajo riesgo: mujeres primigestas, hombres fértiles y mujeres infértiles sin daño tubario. Se determinó en duplicado IgG e IgA anti-NG y anti-CT en suero, por análisis inmunoenzimático, utilizando como antígeno el pili de NG y la fracción L1 de CT. Se calcularon porcentajes. RESULTADOS: En mujeres la prevalencia para NG fue IgG 13.7% e IgA 14.3%, y para CT fue IgG 11.4% e IgA 4.4%. En hombres, NG 3.3% y 13.3%, respectivamente, y para CT 7.2% y 5.5%, respectivamente. En TSC se encontró NG en 31.2 % y 28.4%, respectivamente, y para CT 25.0% y 5.7%, respectivamente. En mujeres con infertilidad por daño tubario, NG 5.6% y 9.8%, respectivamente, y CT 8.4% y 1.4%, respectivamente. En 110 primigestas jóvenes, NG 4.5% y 10.0%, respectivamente, y CT 3.6% y 9.1%, respectivamente. CONCLUSIONES: Estos datos confirman la prevalencia elevada de Neisseria gonorrhoeae y Chlamydia trachomatis en trabajadoras del sexo comercial y en hombres homo/bisexuales, pero no en otros grupos de alto riesgo como las mujeres infértiles, con aborto o embarazo ectópico.OBJECTIVE: To estimate the prevalence of Chlamydia trachomatis (CT and Neisseria gonorrhoeae

  2. Prevalence of HBsAg, knowledge, and vaccination practice against ...

    African Journals Online (AJOL)

    Among the respondents, 56.7% had good knowledge and 94.8% reported poor practice of vaccination against viral hepatitis B infection. Mean knowledge and vaccination practices scores (%) were 72.54+7.60 and 29.44+14.37 respectively. Only 29% of the respondents did post vaccination testing for anti HBsAg.

  3. Prevalencia de anticuerpos contra HTLV-1 en una población negra de Colombia

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    María C. Navas

    1995-03-01

    Full Text Available El virus linfotrópico humano 1 (HTLV-1 ha sido asociado con la leucemia de células T del adulto (ATL y la paraparesia espástica tropical (PET o mielopatía asociada con HTLV-1 (HAM. Aunque la prevalencia de anticuerpos contra el HTLV-1 ha sido descrita en diversos países, especialmente en Japón, poco se conoce acerca de este marcador de contacto viral en nuestra población. En este artículo describimos la ausencia de niveles de anticuerpos anti-HTLV-1 en el suero, medidos por la prueba de ELISA, en una población negra de la costa atlántica de Colombia. Nuestros hallazgos sugieren que: a este grupo descendiente de África no se ha expuesto al virus; b puede ser que algunos de los individuos incluidos en el estudio estén en período de incubación y, que debido a su juventud, no fue posible demostrar una respuesta humoral contra el virus; y, c como ha sido descrito en otros estudios, la prevalencia de anticuerpos anti-HTLV- 1 varía entre las diferentes regiones de Colombia.

  4. Diagnóstico diferencial en la encefalitis por anticuerpos contra el receptor NMDA

    Science.gov (United States)

    González-Valcárcel, J.; Rosenfeld, M.R.; Dalmau, J.

    2011-01-01

    Resumen Introducción La encefalitis por anticuerpos contra el receptor de NMDA (NMDAR) suele desarrollarse como un síndrome característico de evolución multifásica y diagnóstico diferencial amplio. Pacientes Presentamos a 2 pacientes diagnosticadas de encefalitis por anticuerpos NMDAR con un cuadro clínico típico, pero que inicialmente señaló otras etiologías. Discusión La afectación frecuente de pacientes jóvenes con manifestaciones psiquiátricas prominentes indica frecuentemente otras consideraciones diagnósticas; las más frecuentes son las encefalitis virales, los procesos psiquiátricos y el síndrome neuroléptico maligno. Varios síndromes previamente definidos de manera parcial o descriptiva en adultos y pacientes pediátricos probablemente eran casos de encefalitis anti-NMDAR. Conclusiones La encefalitis anti-NMDAR debe considerarse en pacientes jóvenes con manifestaciones psiquiátricas subagudas, movimientos anormales y alteraciones autonómicas. La caracterización clínica e inmunológica de esta enfermedad ha llevado a la identificación de nuevos anticuerpos que afectan a procesos de memoria, aprendizaje, conducta y psicosis. PMID:20964986

  5. UltramicroELISA para la detección de anticuerpos IgM anti M. leprae UltramicroELISA assay for the detection of human IgM antibodies to M. leprae

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    José Laferte

    1991-12-01

    Full Text Available La disponibilidad del sistema Ultramicroanalítico (SUMA y de un antígeno especie-específico del M. leprae obtenido mediante síntesis química, permitió la normalización y validación de un ultramicroELISA para la detección de anticuerpos IgM específicos a esta micobacteria. El análisis de 433 sueros de banco de sangre y 265 sueros usados para validar el método y clasificados en un grupo control de donantes de banco de sangre (100, un grupo de pacientes tuberculosos (50, un grupo de enfermos de lepra (65 y un grupo de contactos de estos enfermos (50, mostró la especificidad del ensayo para evidenciar la infección con el M. leprae. Los resultados obtenidos del estudio adicional de 140 muestras de suero de contactos de enfermos estuvieron estrechamente correlacionados (r = 0,98 con los resultados obtenidos por la técnica de microELISA convencional. La utilización del SUMA no solo permite un notable ahorro de reactivos si no además facilita la lectura, cálculo, validación y almacenamiento automático de los resultados.The availability of an ultramicroanalitic system (SUMA and specie-specific antigen of M. leprae obtained by chemical synthesis, have made possible the standardization and validation of an ultramicroELISA assay for detecting specific human IgM antibodies to this mycobacterium. The specificity of this test to demonstrate the infection with M. leprae was corroborated through a screening of 433 blood bank serum samples and other 265 from diferent groups (100, control group, 50 tuberculosis patients, 65 leprosy patients, 50 from household. The results obtained in the aditional study of 140 household sero showed a high correlation (r = 0.98 with the conventional microELISA method. The use of SUMA allows saving reagents and time since sample handling, plate reading, print out and storing the data are computer assisted.

  6. HBV reactivation with fatal fulminating hepatitis during rituximab treatment in a subject negative for HBsAg and positive for HBsAb and HBcAb.

    Science.gov (United States)

    Sarrecchia, Cesare; Cappelli, Alessandra; Aiello, Pasquale

    2005-08-01

    A 51-year-old man who was hepatitis B surface antigen (HBsAg)-negative and positive for anti-hepatitis B surface antigen (anti-HBs) and anti-hepatitis B core antigen (anti-HBc), during rituximab therapy for chronic Lymphocytic leukemia, developed reactivation of hepatitis B virus (HBV) infection with hepatitis that proceeded towards hepatic failure and death in spite of lamivudine therapy. HBsAg remained persistently negative, notwithstanding a high HBV-DNA titer. Our observation, following other cases of fatal reactivation of HBV infection in patients receiving rituximab, suggests that, in all patients with previous markers of HBV infection, lamivudine prophylaxis should be considered during rituximab therapy.

  7. Presencia de anticuerpos contra neurorreceptores cardiacos de acetilcolina muscarínicos tipo II en pacientes con enfermedad de Chagas e implantación de marcapasos

    Directory of Open Access Journals (Sweden)

    María Clara Echeverry

    2009-09-01

    Conclusión. El odds ratio (OR del presente estudio muestra una mayor probabilidad de presentar anticuerpos anti-m2MAChR en pacientes con implantación de marcapaso e infección por Trypanosoma cruzi, que en aquéllos con implantación que no presentan infección por el parásito. La presencia de anticuerpos anti-m2MAChR no está relacionada con ningún tipo de manifestación clínica de las evaluadas en este estudio.

  8. Obtención de anticuerpos monoclonales de ratón contra proteasa de cisteína 5 recombinante de Entamoeba histolytica

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    Juanita Trejos S.

    2012-08-01

    Full Text Available Objetivo. Obtener anticuerpos monoclonales de ratón contra la proteasas de cisteína 5 (EhCP5 de Entamoeba histolytica. Materiales y métodos. Se inmunizaron ratones BALB/c por vía intraperitoneal con adyuvante de Freund completo e incompleto con la proteína recombinante EhCP5 obtenida a partir del cultivo de E.coli DH5α trasfectada con el vector recombinante pJC45 que expresa dicha proteína. Se seleccionó el animal con mejor respuesta de anticuerpos. Al cual se le extrajo su bazo como fuente de linfocitos B, los cuales se fusionaron utilizando PEG con células de mieloma de ratón SP2-0/Ag14. Se procedió a selección de los hibridomas y a la evaluación de los sobrenadantes de las colonias que crecieron a los 7 días mediante ELISA. Los hibridomas con valores más altos de anticuerpos específicos contra la proteína EhCP5r se seleccionaron, y los clones obtenidos por diluciones limitantes fueron expandidos. Resultados. A partir de un clon secretor estable se purifico el anticuerpo monoclonal anti EhCP5r del isotipo IgG1 por cromatografía de afinidad con proteína G. Los clones fueron expandidos in vivo e in vitro. Con el anticuerpo purificado se diseñaron tres sistemas de captura para evaluar la aplicabilidad del anticuerpo monoclonal anti EhCP5r como método inmunodiagnóstico. Conclusiones. Se logro la producción de un anticuerpo monoclonal específico contra EhCP5r que permite diferenciar Entamoeba histolytica de Entamoeba dispar.

  9. The Lumipulse G HBsAg-Quant assay for screening and quantification of the hepatitis B surface antigen.

    Science.gov (United States)

    Yang, Ruifeng; Song, Guangjun; Guan, Wenli; Wang, Qian; Liu, Yan; Wei, Lai

    2016-02-01

    Qualitative HBsAg assay is used to screen HBV infection for decades. The utility of quantitative assay is also rejuvenated recently. We aimed to evaluate and compare the performance of a novel ultra-sensitive and quantitative assay, the Lumipulse assay, with the Architect and Elecsys assays. As screening methods, specificity was compared using 2043 consecutive clinical routine samples. As quantitative assays, precision and accuracy were assessed. Sera from 112 treatment-naïve chronic hepatitis B patients, four patients undergoing antiviral therapy and one patient with acute infection were tested to compare the correlations. Samples with concurrent HBsAg/anti-HBs were also quantified. The Lumipulse assay precisely quantified ultra-low level of HBsAg (0.004 IU/mL). It identified additional 0.98% (20/2043) clinical samples with trance amount of HBsAg. Three assays displayed excellent linear correlations irrespective of genotypes and S-gene mutations (R(2)>0.95, PLumipulse assay did not yield higher HBsAg concentrations in samples with concomitant anti-HBs. Compared with other assays, the Lumipulse assay is sensitive and specific for detecting HBsAg. The interpretation of the extremely low-level results, however, is challenging. Quantitative HBsAg results by different assays are highly correlated, but they should be interpreted interchangeably only after conversion to eliminate the biases. Copyright © 2015 Elsevier B.V. All rights reserved.

  10. HBsAg-redirected T cells exhibit antiviral activity in HBV-infected human liver chimeric mice.

    Science.gov (United States)

    Kruse, Robert L; Shum, Thomas; Tashiro, Haruko; Barzi, Mercedes; Yi, Zhongzhen; Whitten-Bauer, Christina; Legras, Xavier; Bissig-Choisat, Beatrice; Garaigorta, Urtzi; Gottschalk, Stephen; Bissig, Karl-Dimiter

    2018-04-06

    Chronic hepatitis B virus (HBV) infection remains incurable. Although HBsAg-specific chimeric antigen receptor (HBsAg-CAR) T cells have been generated, they have not been tested in animal models with authentic HBV infection. We generated a novel CAR targeting HBsAg and evaluated its ability to recognize HBV+ cell lines and HBsAg particles in vitro. In vivo, we tested whether human HBsAg-CAR T cells would have efficacy against HBV-infected hepatocytes in human liver chimeric mice. HBsAg-CAR T cells recognized HBV-positive cell lines and HBsAg particles in vitro as judged by cytokine production. However, HBsAg-CAR T cells did not kill HBV-positive cell lines in cytotoxicity assays. Adoptive transfer of HBsAg-CAR T cells into HBV-infected humanized mice resulted in accumulation within the liver and a significant decrease in plasma HBsAg and HBV-DNA levels compared with control mice. Notably, the fraction of HBV core-positive hepatocytes among total human hepatocytes was greatly reduced after HBsAg-CAR T cell treatment, pointing to noncytopathic viral clearance. In agreement, changes in surrogate human plasma albumin levels were not significantly different between treatment and control groups. HBsAg-CAR T cells have anti-HBV activity in an authentic preclinical HBV infection model. Our results warrant further preclinical exploration of HBsAg-CAR T cells as immunotherapy for HBV. Copyright © 2018 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

  11. Prevalencia de anticuerpos antitreponémicos y características sociodemográficas de la población mexicana adulta en el año 2000 Anti-Treponema pallidum seroprevalence and sociodemographic characteristics in Mexican adult population, 2000

    Directory of Open Access Journals (Sweden)

    Carlos J Conde-González

    2007-01-01

    Full Text Available OBJETIVO: Determinar la frecuencia de anticuerpos antitreponémicos en mexicanos adultos de ambos sexos y las características de la población relacionadas con la infección sifilítica. MATERIAL Y MÉTODOS: Se analizaron para anti-Treponema pallidum 12 010 sueros seleccionados de forma aleatoria de la Encuesta Nacional de Salud 2000, con aplicación de un algoritmo conformado por técnicas serológicas treponémicas y no treponémicas, de tamizaje y confirmatorias, y se realizó un análisis estadístico para identificar los factores vinculados con la seropositividad. RESULTADOS: La seroprevalencia de anticuerpos antitreponémicos fue de 3.1% (IC95% 2.5-3.7. Las características relacionadas de modo significativo con la exposición a la infección fueron la mayor edad (RM 3.3, sexo masculino (RM 1.3, residencia en la región geográfica sur del país (RM 1.7, vivir en unión libre (RM 2.7 o ser viudo (RM 1.8, inicio de relaciones sexuales a edad temprana (RM 6.3 y, en las mujeres en particular, se identificó el antecedente de mortinatos (RM 1.8 y el informe de falta de uso del condón como método anticonceptivo (RM 14.1. CONCLUSIONES: Los hallazgos del estudio demuestran que la magnitud del antecedente de la infección por el treponema en la población mexicana adulta es considerablemente mayor respecto del que permite calcular la notificación oficial de casos de la enfermedad. Como sucede con otras infecciones de transmisión sexual, aspectos sociodemográficos, de género y las prácticas sexuales no seguras incrementan la transmisión del agente etiológico. En México existe la necesidad de reforzar la vigilancia de la infección sifilítica a través de un diagnóstico oportuno y eficaz de los casos, más su adecuado tratamiento, para coadyuvar al control del padecimiento.OBJECTIVE: To measure for the first time in a population based study the prevalence of antibodies against Treponema pallidum among the Mexican adult population and

  12. Papel protector de los anticuerpos en la salmonellosis experimental

    OpenAIRE

    Guzmán U., Miguel; Ramírez, Germán

    2011-01-01

    El presente trabajo fue realizado con el propósito de demostrar la capacidad protectora de anticuerpos contra la Salmonella typhi, en la infección experimental en pollos recién nacidos, previamente protegidos con tales anticuerpos.  La cepa de Salmonella typhi usada, tanto para preparar los antígenos O y H como para reproducir experimentalmente la Salmonellosis, fue una cepa Standard de la colección de la Sección de Microbiología de la Facultad de Medicina. Los medios de cultivo usados, tanto...

  13. Distribución heterogénea de la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre en Puebla, México Heterogeneous distribution of the prevalence of anti-Trypanosoma cruzi antibodies among blood donors in the State of Puebla, Mexico

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    Víctor M Monteón

    2005-04-01

    Full Text Available OBJETIVO: Identificar la seroprevalencia de anticuerpos anti Trypanosoma cruzi (Ac anti-T. cruzi en donadores de sangre que habitan en ámbito rural y suburbano, así como las regiones del estado de mayor riesgo y factores asociados. MATERIAL Y MÉTODOS: Estudio transversal realizado de enero a diciembre de 2003. Se analizaron 2 489 donadores de sangre reclutados en 10 puestos de sangrado del Instituto Mexicano del Seguro Social (IMSS distribuidos en las siete regiones económicas del estado de Puebla, México. Se determinó la seroprevalencia mediante las pruebas serológicas obligatorias del panel viral y, además, para T. cruzi, región de reclutamiento y de origen de los donadores. RESULTADOS: La seroprevalencia de Ac anti-T. cruzi fue de 1.24% (31/2 489 comparable con la obtenida para el virus de la hepatitis C (1.5% y por arriba de la del virus de la inmunodeficiencia humana (0.4% y del antígeno de superficie del virus de la hepatitis B (0.3%. Las regiones de Tehuacán-Sierra Negra y Mixteca fueron las de mayor riesgo con seroprevalencias, por el origen del donador, de 2.6% para T. cruzi, mientras que en los originarios de las regiones Sierra nororiental y Angelópolis no se detectaron casos positivos. Se observó asociación entre ser seropositivo y mayor de 40 años y ser originario de las regiones de Tehuacán-Sierra Negra y Mixteca. CONCLUSIONES: La distribución de seroprevalencia a T. cruzi es heterogénea, oscila desde 0% hasta 2.6%, y se reconoce a Tehuacán-Sierra Negra y Mixteca como las regiones de mayor riesgo.OBJECTIVE: To determine the seroprevalence and associated factors, of antibodies against Trypanosoma cruzi (T. cruzi Ab among blood donors living in rural and suburban areas and risk regions. MATERIAL AND METHODS: A cross-sectional study was conducted from January to December 2003, in 2489 blood donors of seven regions of Puebla, who were evaluated for mandatory viral and T. cruzi serological tests using validated

  14. Producción y caracterización de un anticuerpo policlonal dirigido contra la fosfoproteína del virus de la rabia

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    Nadia Yadira Castañeda

    2007-06-01

    Materiales y métodos. El gen P que codifica para la proteína P del virus de la rabia, fue amplificado por reacción en cadena de la polimerasa de transcriptasa reversa y clonado en el vector de expresión PinPointTM Xa-1 T (PROMEGA. La proteína recombinante P fue expresada en E. coli purificada por cromatografía de afinidad y usada para la producción del anticuerpo policlonal anti-P. El anticuerpo obtenido fue purificado y caracterizado por inmunocitoquímica con un sistema enzimático, inmunofluorescencia, Cell-ELISA fluorométrica y Western blotting. Resultados. La proteína recombinante se expresó eficientemente como una proteína de fusión biotinilada de aproximadamente 50 kd, que corresponde a la forma completa de la proteína P del virus de la rabia. El anticuerpo policlonal anti-P detectó con alta especificidad la proteína P en cultivos de neuronas sensoriales infectados con el virus de la rabia. Conclusión. La proteína P recombinante expresada en E. coli se constituyó en un antígeno específico para producir un anticuerpo policlonal que reconoce la proteína P nativa en células infectadas con el virus de la rabia.

  15. Canakinumab: un anticuerpo monoclonal prometedor en el tratamiento de enfermedades cardiovasculares

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    Fernando Manzur, MD., FACC

    2013-01-01

    Full Text Available El canakinumab es un anticuerpo monoclonal anti-IL-1β totalmente humano desarrollado por Novartis, cuyo mecanismo de acción se basa en la neutralización de la señalización IL-1β, lo cual conduce a la supresión de la inflamación en pacientes con trastornos de origen autoinmune. La IL-1β actúa como un mediador de la respuesta inmune periférica durante la infección y la inflamación. Mediante la unión antígeno-anticuerpo el canakinumab inhibe la acción de la IL1-β evitando sus efectos pro-inflamatorios. En la actualidad, está en evaluación como un nuevo posible agente dirigido frente a la IL-1β, con el objetivo de reducir la tasa de eventos cardiovasculares y la diabetes de aparición reciente (estudio CANTOS.

  16. Lack of immune potentiation by complexing HBsAg in a heat-inactivated hepatitis B vaccine with antibody in hepatitis B immunoglobulin

    NARCIS (Netherlands)

    Lelie, P. N.; van Amelsfoort, P. J.; Martine de Groot, C. S.; Bakker, E.; Schaasberg, W.; Niessen, J. C.; Reesink, H. W.

    1989-01-01

    In a randomized, dose-response study among 305 health care workers, we examined whether the immunogenicity of a heat-inactivated hepatitis B vaccine could be enhanced when HBsAg was complexed by anti-HBs contained in hepatitis B immunoglobulin either at equivalent proportions or at 10-fold antigen

  17. Persistence of protective anti-HBs antibody levels and anamnestic response to HBV booster vaccination: A cross-sectional study among healthcare students 20 years following the universal immunization campaign in Italy.

    Science.gov (United States)

    Dini, Guglielmo; Toletone, Alessandra; Barberis, Ilaria; Debarbieri, Nicoletta; Massa, Emanuela; Paganino, Chiara; Bersi, Francesca; Montecucco, Alfredo; Alicino, Cristiano; Durando, Paolo

    2017-02-01

    Vaccination against Hepatitis B Virus (HBV) became mandatory in Italy for all newborns and 12 years-old individuals in the 1991. The immunogenicity of HBV vaccine and the effectiveness of the universal immunization strategy have been widely demonstrated. However the need to assess the antibody concentrations above the well known serological correlate of protection for HBV infection (≥10 mIU/mL), established in individuals immunized with a 3 doses vaccination course, is still recommended in subjects exposed to occupational risks in different settings, particularly the healthcare services. This practice has to be performed during the preventive medical examination, before the worker's exposure to biological hazards, as a fundamental part of Occupational Health Surveillance Programs in several Countries, including Italy: the goal is to assure individual protection, also providing booster doses when needed, after many years following the primary vaccination. During the 2011-2013 period, an observational study was performed in Healthcare students (HCSs) trained at a regional university acute-care hospital in North-Western Italy, properly immunized against HBV during infancy or adolescence, in order to evaluate the persistence of seroprotection and to assess the anamnestic response to booster vaccination. Data from 717 subjects undergoing HbsAg Ab and HBc Ab testing during the preventive medical examination, and receiving a booster dose of HBV vaccine when resulting with a non-protective titer (vaccination course during the first year of life (3-5-11 months). Globally, 507 (70.7%) HCSs showed protective antibody titres, and an anamnestic response was observed in more than 95% subjects receiving the booster dose. Our study demonstrated the long-term persistence of protection of HBV vaccine, more than 20 y following the primary immunization, in HCSs who are exposed to occupational health risk. The anamnestic response observed in non-seroprotected subjects who

  18. Prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre del IMSS, Orizaba, Veracruz, México

    OpenAIRE

    Ramos-Ligonio Angel; Ramírez-Sánchez Michaía Elián; González-Hernández Juan Carlos; Rosales-Encina José Luis; López-Monteon Aracely

    2006-01-01

    OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS) en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO) del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70) y un extracto crudo de epimastigotes. Las muestras fueron o...

  19. Modelo de detección de anticuerpos neutralizantes contra IFN-β mediante citometría de flujo

    Directory of Open Access Journals (Sweden)

    Juan Carlos Villa-Camacho

    2012-12-01

    Full Text Available Introducción. El interferón beta (IFN-β se usa para tratar la forma recaída-remisión de la esclerosis múltiple. Sin embargo, el uso de proteínas recombinantes como medicamentos puede generar la producción de anticuerpos, disminuyendo así la efectividad del tratamiento. Objetivo. Estandarizar una técnica de detección de anticuerpos neutralizantes contra IFN-β, mediante citometría de flujo. Materiales y métodos. Se cultivaron dos líneas tumorales humanas (U937 y K562 con IFN-β1a humano recombinante y mediante citometría de flujo se determinó la expresión de la proteína ISG15 intracelular. Los sueros se obtuvieron de un conejo Nueva Zelanda antes y después de la inmunización con 100.000 UI de IFN-β1a en adyuvante de Freund. Para la detección de anticuerpos neutralizantes, se estimularon células K562 con IFN-β1a preincubado con sueros de conejos a una dilución 1:20. Después de 24 horas de incubación se determinó la expresión de la proteína ISG15. Resultados. La expresión de ISG15 fue mayor en células K562 estimuladas. La intensidad media de fluorescencia para la ISG-15 entre células en ausencia IFN-β1a, mostró una mediana de 198 unidades arbitrarias (UA (p25-75= 173-231 UA y, en presencia de IFN-β1a, 430 UA (p25-75 =316-611,5, con una diferencia estadísticamente significativa (p=0,008. La presencia de anticuerpos anti-IFN-β1a en el suero del conejo inmunizado, hizo disminuir de forma acentuada la expresión de la ISG15 en células K562 cultivadas con IFN-β1a, en comparación con el control (mediana=3,040 UA Vs. 43,644 UA, respectivamente. Conclusiones. Este trabajo muestra la detección de anticuerpos neutralizantes contra IFN-β en conejos, utilizando la expresión de la proteína ISG15 por citometría de flujo.   doi: http://dx.doi.org/10.7705/biomedica.v32i4.637

  20. Papel protector de los anticuerpos en la salmonellosis experimental

    Directory of Open Access Journals (Sweden)

    Miguel Guzmán U.

    1967-01-01

    Full Text Available El presente trabajo fue realizado con el propósito de demostrar la capacidad protectora de anticuerpos contra la Salmonella typhi, en la infección experimental en pollos recién nacidos, previamente protegidos con tales anticuerpos.  La cepa de Salmonella typhi usada, tanto para preparar los antígenos O y H como para reproducir experimentalmente la Salmonellosis, fue una cepa Standard de la colección de la Sección de Microbiología de la Facultad de Medicina. Los medios de cultivo usados, tanto para preparar los antígenos como para aislar los microorganismos de los animales inoculados fueron medios usuales, recomendados para el estudio de Enterobacteriaceas; Endo McConkey y Agar-sangre.

  1. Frecuencia de anticuerpos contra el virus C de la hepatitis en pacientes con cirrosis hepática en Yucatán, México Frequency of antibodies against the hepatitis C virus in patients with hepatic cirrhosis in Yucatan, Mexico

    Directory of Open Access Journals (Sweden)

    Renán A Góngora-Biachi

    2003-10-01

    Full Text Available OBJETIVO: En este estudio reportamos la prevalencia de anticuerpos contra el virus C de la hepatitis (Ac-VCH en un grupo de pacientes con cirrosis hepática (CH. MATERIAL Y MÉTODOS: Se hizo un estudio prospectivo, transversal y descriptivo, de marzo de 1998 a mayo de 1999. Se estudiaron a 153 pacientes (117 (76% hombres y 36 (24% mujeres con diagnóstico de CH, que eran atendidos en el Hospital General Agustín O' Horan y en el Centro de Investigaciones Regionales Doctor Hideyo Noguchi, en la ciudad de Mérida, Yucatán, México. Se aplicó un cuestionario con datos clínico-epidemiológicos y se determinó la presencia de Ac-VCH (ELISA de 2ª generación y RIBA-2 para confirmar el diagnóstico a cada paciente. Se determinó también el antígeno de superficie de la hepatitis B (AgsHB y anticuerpos contra el antígeno central de la hepatitis B (Anti-HBc mediante el método de ELISA. La presencia de Ac-VCH fue relacionada con las variables epidemiológicas de los sujetos. La prevalencia de anti-HCV y la frecuencia de características se compararon entre los pacientes positivos y negativos con las pruebas de c² y exacta de Fisher. RESULTADOS: El 32% de los pacientes con CH (35/117 (30% hombres y 14/36 (39% mujeres fueron positivos para los Ac-VCH. El alcoholismo estuvo presente en todos los hombres serorreactivos y en ninguna de las mujeres positivas (pOBJECTIVE: To report the prevalence of antibodies against the hepatitis C virus (anti-HCV in a group of patients with hepatic cirrhosis (HC. MATERIAL AND METHODS: A prospective transversal and descriptive study was carried out from March 1998 to May 1999. Study subjects were 153 patients; 117 (76% male and 36 (24% female, diagnosed with HC. They were attended at the General Hospital Agustín O' Horan and at Regional Research Center Doctor Hideyo Noguchi, in Merida, Yucatan, Mexico. A clinical-epidemiologic questionnaire completed by interview was used for data collection. Anti-HCV were detected

  2. Protective effect of hepatitis B vaccine combined with two-dose hepatitis B immunoglobulin on infants born to HBsAg-positive mothers.

    Science.gov (United States)

    Zou, Huaibin; Chen, Yu; Duan, Zhongping; Zhang, Hua

    2011-01-01

    Despite the use of hepatitis B (HB) vaccine and hepatitis B immunoglobulin (HBIG), a portion of infants are still non- or low-responders, or even immunoprophylaxis failure. We aimed to determine the immune response in the infants from the mothers being positive for hepatitis B surface antigen (HBsAg), by which the infants received three doses of HB vaccine in combination with two-dose 200 IU HBIG injections. In this retrospective study, 621 infants from HBsAg-positive mothers in Beijing YouAn Hospital between January 2008 and December 2009 were included. All the infants were given three doses of 10 µg HB vaccine (at 0, 1 and 6 months of age) and two-dose of 200 IU HBIG (at birth and in 2 weeks of age). Serum HBsAg and antibody to HBsAg (anti-HBs) in all the infants were determined at 7 months of age. Of the 621 infants, 2.9% were immunoprophylaxis failure (positive for HBsAg), 1.4% were non-responders (anti-HBs undetectable), 95.7% were responders. The 594 responders could be categorized into three subsets, 22 were 10 to 99 IU/L for anti-HBs levels, 191 were 100 to 999 IU/L, and 381 were ≥1000 IU/L. The immunoprophylaxis failure rate was at 0% and 5.2% for the infants of HBeAg-negative and HBeAg-positive mothers(Pvaccination. Through vaccination by three doses of HB and two-dose of HBIG, majority of the infants (95.7%) achieved a protective level of anti-HBs at 7 months of age. Maternal HBeAg-positive and HBV DNA detectable were associated with the immunoprophylaxis failure, but not contribute to the non- or low-response to HB vaccination.

  3. Marcadores inflamatórios e anticorpos anti-chlamydia em pacientes com síndrome metabólica Marcadores inflamatorios y anticuerpos anti-chlamydia en pacientes con síndrome metabólico Inflammatory markers and antichlamydial antibodies in patients with metabolic syndrome

    OpenAIRE

    Rosecler Riethmuller Franco; Luiz Carlos Bodanese; Giuseppe Repetto; Jacqueline da Costa Escobar Piccoli; Mario Wiehe; Cassiane Bonato; Marta Maria Medeiros Frescura Duarte; Thiago Duarte

    2011-01-01

    FUNDAMENTO: A síndrome metabólica está associada ao aumento de risco de eventos cardiovasculares. Marcadores inflamatórios e anticorpos anti-Chlamydia têm sido relacionados ao desenvolvimento e à progressão da aterosclerose e dos eventos cardiovasculares. OBJETIVO: Avaliar os marcadores inflamatórios interleucina-6 (IL-6) e fator de necrose tumoral-alfa (TNF-α) e os anticorpos anti-Chlamydia pneumoniae em pacientes com síndrome metabólica (SM), com e sem eventos cardiovasculares. MÉTODOS...

  4. Antisperm autoantibodies and infertility Anticuerpos antiespermatozoides e infertilidad

    Directory of Open Access Journals (Sweden)

    Jorge Eliécer Ossa Londoño

    1997-02-01

    Full Text Available Fourteen human infertile couples were studied along with seven fertile control couples in order to determine the presence of antisperm au. toantibodies and to correlate results with their clinical reproductive status, spermogram parameters and the quality of cervical mucus. Immunoglobulins of IgG and IgA isotypes were evaluated on spermatozoa by the direct immunobead technique and in serum and cervical mucus by the indirect method. The following differences were found: Nine of 14 male patients but only 1 of 7 male controls had IgG on spermatozoa (p= 0.042 and OR= 10.8. Seven out of 14 male patiens and 1 of 7 controls were positive for IgA on spermatozoa (p= 0.12. Infertile males had a higher frequency of abnormal spermogram parameters as compared to controls (p= 0.0029 and OR= 3.32. Interestingly, the presence of only IgG in serum appeared to be a "protective factor" against IgG on sperm; only one out of the 16 positive males contradicted this observation. Para determinar la presencia de anticuerpos antiespermatozoides y correlacionarla con la situación reproductiva, el espermograma y la calidad del moco cervical, se estudiaron 7 parejas sanas, con hijos, y 14 infértiles. Se determinaron por las técnicas directa e indirecta de inmunoesferas los anticuerpos de los isotipos IgG e IgA en espermatozoides, suero y moco cervical. En 9 de los 14 hombres infértiles y en 1 de los 7 fértiles se encontraron anticuerpos IgG en la superficie de los espermatozoides (p= 0.042 y una Razón de disparidad [RD] = 10.8 y 8 de éstos también tuvieron IgA en los espermatozoides. El número de pruebas alteradas en el espermograma de los hombres infértiles fue significativamente mayor (p= 0.0029 y RD = 3.32. Tanto en los hombres infértiles como en los fértiles la presencia de sólo IgG en el suero parece ser un "factor de protección" contra IgG en los espermatozoides; de los 16 hombres que presentaron anticuerpos, fuera en el suero o en los espermatozoides, s

  5. Potential infectivity of blood from HBsAG asymptomatic carriers due to the presence of HBV-DNA and comparison with other markers of hbv infection Potencial de infectividade em sangue de portadores assintomáticos de HBsAg caracterizado pela presença de HBV-DNA e comparação com outros marcadores da Hepatite B.

    Directory of Open Access Journals (Sweden)

    M. Valeria Tedeschi

    1989-12-01

    Full Text Available Serum samples from 356 HBsAg positive asymptomatic carriers, which were titrated by reverse passive hemagglutination, were analysed for the presence of HBV-DNA, HBsAg and IgM anti-HBc. The samples were divided in three classes, according to the titers of HBsAg and IgM anti-HBc and the distribution of HBV-DNA and HBsAg among these classes was studied. In the high titer class of HBsAg, 65% of samples have one or both markers against only 19% in the low titer class. From the total of 356 samples, 121 gave positive results for IgM anti-HBc (33.9%. From these, 38.9% of HBV-DNA and 47.9% of HBeAg were observed, whereas in samples with absence of IgM anti-HBc, 18.3% and 16.6% were respectively found. A higher frequency of agreement between all these markers was found in the class of high titers of HBsAg; however, HBV-DNA was detected in the low titer class of HBsAg and little or no IgM anti-HBc, showing potential blood infectivity even in HBsAg positive borderline samples.Em 356 soros HBsAg positivos de portadores assintomáticos, titulados por hemaglutinação passiva reversa, foi analisada a presença de HBV DNA, HBeAg e IgM anti-HBc, da seguinte forma: as amostras foram divididas em três classes de acordo com o título de HBsAg e IgM anti-HBc e foi verificada a distribuição de HBV DNA e HBsAg nestas classes. Em amostras com títulos elevados de HBsAg, 65% das amostras tiveram um ou ambos os marcadores HBV-DNA e HBsAg e as mesmas foram encontradas em apenas 19% das amostras com baixo título de HBsAg. Do total de 356 amostras, 121 foram positivas para IgM anti-HBc (33.9%. Destas, 38.9% de HBV-DNA e 47.9% de HBeAg foram observadas, ao passo que em amostras com ausência de IgM anti-HBc, 18.3% e 16.6% foram respectivamente encontrados. A concordância entre os três marcadores foi encontrada em. amostras com alto título de HBsAg, entretanto HBV-DNA foi também detectado em grupo de baixo título de HBsAg na ausência de IgM anti-HBc ou em n

  6. Posintro™-HBsAg, a modified ISCOM including HBsAg, induces strong cellular and humoral responses

    DEFF Research Database (Denmark)

    Schiött, Asa; Larsson, Kristina; Manniche, Søren

    2011-01-01

    HBsAg vaccine formulation, Posintro™-HBsAg, was compared to two commercial hepatitis B vaccines including aluminium or monophosphoryl lipid A (MPL) and the two adjuvant systems MF59 and QS21 in their efficiency to prime both cellular and humoral immune responses. The Posintro™-HBsAg induced...... of delayed type hypersensitivity (DTH) reaction and CD4(+) T-cell proliferation. In addition, Posintro™-HBsAg was the only vaccine tested that also induced a strong cytotoxic T lymphocyte (CTL) response, with high levels of antigen specific CD8 T-cells secreting IFN-gamma mediating cytolytic activity...

  7.  Active-passive Immunization Effectiveness Against Hepatitis B Virus in Children Born to HBsAg Positive Mothers in Amol, North of Iran

    Directory of Open Access Journals (Sweden)

    2011-11-01

    Full Text Available  Objectives: HBV infection is a contagious disease that may transmit vertically from mothers to their neonates or horizontally by blood products and body secretions. Over 50�0of Iranian carriers have contracted the infection perinatally, making this the most likely route of transmission of HBV in Iran. This study assesses the serologic markers of HBV in children born to HBsAg positive mothers who received HBIG and 3 doses of HBV vaccine. Methods: To evaluate the effectiveness of vaccination against HBV, a study was conducted on 95 Children, born to hepatitis B surface antigen (HBsAg-positive mothers, who had received Hepatitis B Immune Globulin and HBV vaccines during 2004-2008. All children were tested for the presence of HBsAg, anti-HBs and anti-HB core antigen (anti-HBc. Results: Among an estimated 30000 pregnant women during the five year study, about 130 (0.42�20were HBV carriers. Ninety-five children from these mothers were enrolled in this study. Only one child (1.1�20was HBsAg positive, while 88.4�0of children were Anti-HBs Positive. Eleven children (11.6�20were exposed to HBV as shown by the presence of anti-HBc. A significant difference was observed between the children’s age and Anti-HBs (p=0.0001. Conclusion: Passive-active immunoprophylaxis of high risk babies was highly efficacious in preventing perinatal transmission of the HBV carrier state. Also, evaluation of serologic markers in HBV infected people is important for designing the strategies for disease control.

  8. Evaluation of a novel cold storage solution (HBs) in a rat kidney transplant model.

    Science.gov (United States)

    Lopez-Neblina, Fernando; Toledo, Alexander H; Toledo-Pereyra, Luis H

    2007-01-01

    We developed an improved solution for hypothermic storage (0-4 degrees C) of kidneys. The cold storage solution (HBS) was composed of macromolecules, high-energy cellular substrates, and a mixture of antiproteolytic amino acids, antioxidants, and anti-inflammatory compounds. The objectives in developing this solution were to achieve superior metabolic support of the kidney during cold storage and to protect against ischemic injury. Inbred Brown Norway rats, weighing 225-250 g, were subjected to orthotopic ultrarapid technique for kidney isotransplantation to minimize warm ischemia and to test the preservation process. The kidney was transplanted after 12 h of preservation. The animals were divided into three groups based upon the preservation solution utilized: HBS solution, HTK solution (Custodiol), and UW solution (UWS)(ViaSpan). Among the recipients, each group had two subsets. The first subset of animals was used to assess survival at 7 days as well as the reperfusion damage index (RDI) based on the macroscopic physical characteristics of the kidney at the time of transplantation. The second subset in each group was utilized to measure serum creatinine and blood urea nitrogen at 4 and 7 days, and histology at death or sacrifice. Mean +/- standard deviation (M +/- SD) was used for all parameters studied. The HBS solution showed significantly better protection at 12 h when compared to HTK and UW solutions. The reperfusion damage index (RDI) showed excellent preservation in the HBS (14 +/- 1), good preservation in UWS (13 +/- 1.5), and moderate preservation in the HTK (11 +/- 2) group. Histology was in concordance with the RDI, showing better histological findings with HBS and UW solutions than with the HTK group. Serum creatinine was significantly better in the HBS group when compared to HTK and UWS. Survival was statistically different, with 80% survival at 7 days in the HBS group, 20% survival in the HTK group, and 50% survival in the UWS group (p solution

  9. Viral hepatitis and rapid diagnostic test based screening for HBsAg in HIV-infected patients in rural Tanzania.

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    Fabian C Franzeck

    Full Text Available BACKGROUND: Co-infection with hepatitis B virus (HBV is highly prevalent in people living with HIV in Sub-Saharan Africa. Screening for HBV surface antigen (HBsAg before initiation of combination antiretroviral therapy (cART is recommended. However, it is not part of diagnostic routines in HIV programs in many resource-limited countries although patients could benefit from optimized antiretroviral therapy covering both infections. Screening could be facilitated by rapid diagnostic tests for HBsAg. Operating experience with these point of care devices in HIV-positive patients in Sub-Saharan Africa is largely lacking. We determined the prevalence of HBV and Hepatitis C virus (HCV infection as well as the diagnostic accuracy of the rapid test device Determine HBsAg in an HIV cohort in rural Tanzania. METHODS: Prospectively collected blood samples from adult, HIV-1 positive and antiretroviral treatment-naïve patients in the Kilombero and Ulanga antiretroviral cohort (KIULARCO in rural Tanzania were analyzed at the point of care with Determine HBsAg, a reference HBsAg EIA and an anti-HCV EIA. RESULTS: Samples of 272 patients were included. Median age was 38 years (interquartile range [IQR] 32-47, 169/272 (63% subjects were females and median CD4+ count was 250 cells/µL (IQR 97-439. HBsAg was detected in 25/272 (9.2%, 95% confidence interval [CI] 6.2-13.0% subjects. Of these, 7/25 (28% were positive for HBeAg. Sensitivity of Determine HBsAg was rated at 96% (95% CI 82.8-99.6% and specificity at 100% (95% CI, 98.9-100%. Antibodies to HCV (anti-HCV were found in 10/272 (3.7%, 95% CI 2.0-6.4% of patients. CONCLUSION: This study reports a high prevalence of HBV in HIV-positive patients in a rural Tanzanian setting. The rapid diagnostic test Determine HBsAg is an accurate assay for screening for HBsAg in HIV-1 infected patients at the point of care and may further help to guide cART in Sub-Saharan Africa.

  10. Study on quantification of HBs-antibody by immunoradiometric assay

    International Nuclear Information System (INIS)

    Kondo, Yuichi; Itoi, Yoshihiro; Kajiyama, Shizuo

    1989-01-01

    Quantification of HBs-antibody assay was carried out using a commercialized assay kit and standard solutions of HBs-antibody recognised as 1 st reference preparation of hepatitis B immunogloblin by WHO. Standard curve of HBs-antibody was drawn with the function of 3D-spline and the correlation factor was obtained as r = 0.999. Coefficient of intra-assay variance was 3.8 % and that of inter-assay variance was 7.8 %. Dilution tests showed satisfactory results in the range of 2-16 times. Correlation between value of cut-off indices and concentration of HBs-antibody was obtained as the formula of y = 2.599 x-3.894 (r = 0.992) and 2.1 of cut-off index corresponded to about 5 mIU/ml of HBs-antibody concentration. (author)

  11. Generation of murine triomas secreting bi-specific monoclonal antibodies that recognize HBsAG ad and ay subtypes.

    Science.gov (United States)

    Falero, G; Rodríguez, I; Sarracent, J; Otero, A J; Rodríguez, B L; Rojas, A; Ochoa, E

    1992-12-01

    We report the generation of murine triomas by fusing splenocytes from mice previously immunized with HBsAg ay-subtype and a hybridoma, secreting anti-HBsAg ad-subtype monoclonal antibody, which was rendered HGPRT- by induced mutagenesis with N-methyl-N'nitro-N-nitrosoguanidine. The fusion yielded a 83.8% of hybrids showing the antigen specificity of the parental hybridoma and a 16.1% of bi-specific monoclonal antibodies. One of them, coded as 1C8A5, showing a heavy chain isotype (IgG1/IgG2b) was used as capture reagent in an ultramicro-ELISA. As little as 0.78 I.U. of both HBsAg ad- and ay-subtypes could be realiably detected.

  12. Detección de pepsinógeno mediante el uso de antisueros y anticuerpos monoclonales.

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    Oscar Orozco

    2000-02-01

    Full Text Available

    El pepsinógeno (PGA y PGC es una aspartato proteasa secretada por células principales gástricas que a pH ácido se convierte en pepsina. Los niveles séricos de dicha enzima se han postulado como indicadores del estado de la mucosa gástrica ya que dependiendo de la patología de base estos pueden encontrarse elevados o disminuidos. Así, en la gastritis superficial y úlcera duodenal los niveles de PGA se encuentran aumentados, mientras que en la gastritis atrófica y cáncer gástrico se encuentran disminuidos. Se planteó como objetivo de este trabajo producir anticuerpos policlonales y monoclonales anti-pepsinógeno con el fin de ser empleados en el diseño de sistemas de cuantificación de pepsinógeno sérico, ya que los sistemas de cuantificación comerciales son muy costosos y de difícil acceso. Para la producción de los anticuerpos se empleó pepsina A porcina como inmunógeno teniendo en cuenta que presenta una alta homología en la secuencia de aminoácidos (86% con la pepsina A humana y además tienen una estructura secundaria idéntica. Anticuerpos policlonales fueron obtenidos de conejos Nueva Zelanda inmunizados vía subcutánea con pepsina A porcina y los anticuerpos monoclonales a partir de células de bazo de los ratones BALB/c inmunizados con pepsina A vía intraperitoneal. Los anticuerpos tanto policlonales como monoclonales fueron purificados por precipitación con sulfato de amonio y posteriormente cromatografía de intercambio iónico a partir del suero o líquido ascítico respectivamente. Los anticuerpos purificados, tanto policlonales como monoclonales, son capaces de reconocer pepsina porcina y pepsinógeno humano presente en sonicados de biopsias gástricas en ensayos de ELISA y western blot. En ensayos de inmunohistoquímica realizados sobre cortes de biopsias gástricas los anticuerpos monoclonales detectan el pepsinógeno presente en el

  13. Understanding early serum hepatitis D virus and HBsAg kinetics during pegylated interferon-alfa therapy via mathematical modeling

    Science.gov (United States)

    Guedj, Jeremie; Rotman, Yaron; Cotler, Scott J.; Koh, Christopher; Schmid, Peter; Albrecht, Jeff; Haynes-Williams, Vanessa; Liang, Jake T.; Hoofnagle, Jay H.; Heller, Theo; Dahari, Harel

    2014-01-01

    There is little information on the early kinetics of hepatitis delta virus (HDV) and hepatitis B surface antigen (HBsAg) during interferon-α therapy. Here a mathematical model was developed and fitted to frequent HDV and HBsAg kinetic data from 10 patients during the first 28 weeks of pegylated-interferon-α2a (peg-IFN) therapy. Three patients achieved a complete virological response (CVR), defined as undetectable HDV 6 months after treatment stopped with loss of HBsAg and anti-HBsAg seroconversion. After initiation of therapy a median delay of 9 days (interquartile range IQR:[5;15]) was observed with no significant changes in HDV level. Thereafter, HDV declined in a biphasic manner, where a rapid first-phase lasting for 25 days (IQR:[23;58]) was followed by a slower or plateau second-phase. The model predicts that the main effect of peg-IFN is to reduce HDV production/release with a median effectiveness of 96% (IQR:[93;99.8]). Median serum HDV half-life (t1/2) was estimated to 2.9 days (IQR:[1.5;5.3]) with pretreatment production and clearance of about 1010 (IQR:[109.8-1010.8]) virions/day. None of the patients with flat 2nd phase in HDV achieved CVR. HBsAg kinetics of decline paralleled the second-phase of HDV decline consistent with HBsAg-productive-infected cells being the main source of production of HDV, with a median t1/2 of 135 days (IQR:[20-460]. The interferon lambda-3 polymorphism (rs12979860) was not associated with kinetic parameters. Conclusions Modeling results provide insights into HDV-host dynamics, the relationship between serum HBsAg levels and HBsAg-infected cells, IFN's mode of action and its effectiveness. The observation that a flat second phase in HDV and HBsAg kinetics was associated with failure to achieve CVR provides the basis to develop early stopping rules during peg-IFN treatment in HDV-infected patients. PMID:25098971

  14. [Clinical evaluation of a novel HBsAg quantitative assay].

    Science.gov (United States)

    Takagi, Kazumi; Tanaka, Yasuhito; Naganuma, Hatsue; Hiramatsu, Kumiko; Iida, Takayasu; Takasaka, Yoshimitsu; Mizokami, Masashi

    2007-07-01

    The clinical implication of the hepatitis B surface antigen (HBsAg) concentrations in HBV-infected individuals remains unclear. The aim of this study was to evaluate a novel fully automated Chemiluminescence Enzyme Immunoassay (Sysmex HBsAg quantitative assay) by comparative measurements of the reference serum samples versus two independent commercial assays (Lumipulse f or Architect HBsAg QT). Furthermore, clinical usefulness was assessed for monitoring of the serum HBsAg levels during antiviral therapy. A dilution test using 5 reference-serum samples showed linear correlation curve in range from 0.03 to 2,360 IU/ml. The HBsAg was measured in total of 400 serum samples and 99.8% had consistent results between Sysmex and Lumipulse f. Additionally, a positive linear correlation was observed between Sysmex and Architect. To compare the Architect and Sysmex, both methods were applied to quantify the HBsAg in serum samples with different HBV genotypes/subgenotypes, as well as in serum contained HBV vaccine escape mutants (126S, 145R). Correlation between the methods was observed in results for escape mutants and common genotypes (A, B, C) in Japan. Observed during lamivudine therapy, an increase in HBsAg and HBV DNA concentrations preceded the aminotransferase (ALT) elevation associated with drug-resistant HBV variant emergence (breakthrough hepatitis). In conclusion, reliability of the Sysmex HBsAg quantitative assay was confirmed for all HBV genetic variants common in Japan. Monitoring of serum HBsAg concentrations in addition to HBV DNA quantification, is helpful in evaluation of the response to lamivudine treatment and diagnosis of the breakthrough hepatitis.

  15. Occult HBV infection in HCC and cirrhotic tissue of HBsAg-negative patients: a virological and clinical study.

    Science.gov (United States)

    Coppola, Nicola; Onorato, Lorenzo; Iodice, Valentina; Starace, Mario; Minichini, Carmine; Farella, Nunzia; Liorre, Giulia; Filippini, Pietro; Sagnelli, Evangelista; de Stefano, Giorgio

    2016-09-20

    To evaluate the virological and clinical characteristics of occult HBV infection (OBI) in 68 consecutive HBsAg-negative patients with biopsy-proven cirrhosis and HCC. HBV DNA was sought and sequenced in plasma, HCC tissue and non-HCC liver tissue by PCRs using primers for HBV core, surface and x regions. OBI was identified by the presence of HBV DNA in at least two different PCRs. OBI was detected in HCC tissue of 13 (20%) patients and in non-HCC liver tissue of 3 of these 13. OBI was detected in HCC tissue of 54.5% of 11 anti-HBs- negative/anti-HBc-positive patients, in 29.4% of 17 anti-HBs/anti-HBc-positive and in 5% of 40 anti-HBs/anti-HBc-negative (p HCC tissue more frequently than the 55 without showed Child-B or -C cirrhosis (53.9% vs. 5.5%, p HCC tissue showed amino acid (AA) substitutions (F19L, P24L, S59F, T131I, Q129H) and deletions (in positions 4,8, 17 and 86) in the S region, AA substitutions (T40S, P124K, L54P, G76A, N222T and I273L) in pre-S1 region and AA substitutions in pre-S2 region (P41H and P66L). In the 3 patients showing OBI also in non-HCC liver tissue the S, pre-S1 and pre-S2 sequencing displayed patterns of mutations different. The study showed a significant correlation between OBI and the severity of liver damage, several patterns of mutations in the S, pre-S1 and pre-S2 regions in HCC tissue, some at their first description.

  16. HCV and HBV coexist in HBsAg-negative patients with HCV viremia; possibility of coinfection in these patients must be considered in HBV-high endemic area

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Dong Soon [Korea Cancer Center Hospital, Seoul (Korea, Republic of)

    1998-01-01

    Hepatocellular carcinoma (HCC) is one of the most common cancers and is highly associated with HBV infection in Korea. It has been suggested that HCV core protein may impair the polymerase activity of HBV in vitro, potentially lowering HBV titre in coinfected patients. The aim of this study was to confirm the coexistence of HBV viremia in HCV infected patients HCC who have apparent HBsAg seronegativity. The serological profiles of HBV and HCV in 616 patients with HCC were analysed and coinfection rate of HBV and HCV investigated. Sera were obtained from 16 patients who were both anti-HCV and HCV RNA positive but HbsAg negative, and tested for HBV BY PCR. As a control group, sera were obtained from 15 patients with HCC and 30 non-A abd non-B chronic hepatitis patients without HCC; both were anti-HCV, HCV-RNA, and HBsAg negative and tested for HBV PCR. Of 616 patients with HCC, 450 (73.1 %) had current HBV infection, 48 (7.8 %) had anti-HCV antibodies, and nine (1.5 %) had viral markers of both HCV abd HBV by serological profiles. Of 27 the patients with HCV viremia and HBsAg seronegativity, 14 (51.9 %) showed HBV viremia by PCR. In contrast, of the 75 patients in the control group who were both HCV PCR negative and HBsAg negative, five (11.1 %) showed HBV viremia by PCR. The PCR for HBV revealed coexistent HBV viremia in HCV viremia patients, despite HBsAg negativity by EIA. In HBV-endemic areas, the possibility of coinfection of HBV in HBsAg-negative patients with HCV viremia should be considered and molecular analysis for HBV-DNA performed. (author). 18 refs., 4 tabs.

  17. ¿Es necesario investigar anticuerpos IgM contra el virus de la hepatitis A cuando el enzimograma hepático es normal?

    Directory of Open Access Journals (Sweden)

    C. Coitinho

    Full Text Available La hepatitis por virus A (VHA es la más frecuente de las hepatitis virales en el mundo, especialmente en los países subdesarrollados. Donde esta enfermedad es endémica, se suelen realizar un gran número de estudios de laboratorio para confirmarla. El objetivo del presente trabajo fue evaluar la utilidad de investigar anticuerpos IgM anti hepatitis A (IgM anti-VHA para el diagnóstico de la VHA en pacientes con niveles séricos normales de aspartato y alanina aminotransferasas (AST/ALT. Todos los pacientes que concurrieron al laboratorio con solicitud de enzimograma hepático y anticuerpos IgM anti-VHA durante el período octubre 2005-marzo 2006 fueron evaluados en este estudio. Los datos clínicos más frecuentes fueron presunción de hepatitis e ictericia (27,5 y 12,7%. La determinación de IgM anti-VHA se realizó por ensayo inmunoenzimático de micropartículas (MEIA; la de enzimas hepáticas en un multianalizador. De los 158 pacientes estudiados, 84 tenían valores elevados de AST/ALT; dentro de este grupo, el 82% fue reactivo para IgM anti-VHA. Los 74 pacientes restantes mostraron niveles normales de AST/ALT y solo 7 de ellos, bajo control de evolución por VHA ya confirmada, fueron reactivos para IgM anti-VHA. El 49% de los pacientes IgM anti-VHA reactivos correspondió a menores de 10 años. De acuerdo con estas observaciones, sugerimos que los estudios de laboratorio para confirmar infección aguda por VHA deberían realizarse en forma secuencial, ya que es innecesaria la determinación de anticuerpos IgM anti-VHA cuando las aminotransferasas séricas son normales.

  18. Protective effect of hepatitis B vaccine combined with two-dose hepatitis B immunoglobulin on infants born to HBsAg-positive mothers.

    Directory of Open Access Journals (Sweden)

    Huaibin Zou

    Full Text Available Despite the use of hepatitis B (HB vaccine and hepatitis B immunoglobulin (HBIG, a portion of infants are still non- or low-responders, or even immunoprophylaxis failure. We aimed to determine the immune response in the infants from the mothers being positive for hepatitis B surface antigen (HBsAg, by which the infants received three doses of HB vaccine in combination with two-dose 200 IU HBIG injections.In this retrospective study, 621 infants from HBsAg-positive mothers in Beijing YouAn Hospital between January 2008 and December 2009 were included. All the infants were given three doses of 10 µg HB vaccine (at 0, 1 and 6 months of age and two-dose of 200 IU HBIG (at birth and in 2 weeks of age. Serum HBsAg and antibody to HBsAg (anti-HBs in all the infants were determined at 7 months of age.Of the 621 infants, 2.9% were immunoprophylaxis failure (positive for HBsAg, 1.4% were non-responders (anti-HBs undetectable, 95.7% were responders. The 594 responders could be categorized into three subsets, 22 were 10 to 99 IU/L for anti-HBs levels, 191 were 100 to 999 IU/L, and 381 were ≥1000 IU/L. The immunoprophylaxis failure rate was at 0% and 5.2% for the infants of HBeAg-negative and HBeAg-positive mothers(P<0.001. Infants from mothers with detectable HBV DNA had higher incidence of immunoprophylaxis failure than those of mothers without detectable HBV DNA (P = 0.002. The factors including gender, birth weight, gestation weeks, the rates of maternal HBeAg-positive, and detectable HBV DNA did not contribute to the no response to HB vaccination.Through vaccination by three doses of HB and two-dose of HBIG, majority of the infants (95.7% achieved a protective level of anti-HBs at 7 months of age. Maternal HBeAg-positive and HBV DNA detectable were associated with the immunoprophylaxis failure, but not contribute to the non- or low-response to HB vaccination.

  19. ELISA Ureasa para selección primaria y monitoreo de anticuerpos monoclonales

    Directory of Open Access Journals (Sweden)

    Gladys Thalía Cortes

    1990-12-01

    Full Text Available Se empleó el método de Elisa Ureasa indirecto para detectar anticuerpos monoclonales obtenidos a partir de ratones inmunizados con merozoítos libres de Plasmodium falciparum. Se seleccionaron los anticuerpos monoclonales que mostraron ser muy estables en posteriores ensayos de caracterización. Los anticuerpos obtenidos no fueron específiCos contra antígeno de Plasmodium falciparum pero permitieron identificar la proteína Espectrina del citoesqueleto de eritrocito humano. En el ensayo fue empleado un antígeno de esquizontes concentrados y solubilizados. Se obtuvo la determinación del punto final de la reacción a través de lectura visual semicuantitativa. A partir de la enzima Ureasa de alta pureza que se encuentra disponible comercialmente y, empleando métodos muy sencillos se prepararon los reactivos utilizados en el ensayo.

  20. The Study on the Prevalence and Significance of Urinary HBsAg Detection

    International Nuclear Information System (INIS)

    Han, Jin Suk; Pyo, Heui Jung; Shin, Young Tae; Park, Jeong Sik; Kim, Suhng Gwon; Choe, Kang Won; Lee, Jung Sang; Lee, Mon Ho

    1981-01-01

    HBsAg. was identified in the urine of the patients positive for serum HBsAg. by Tripatzis in 1970. In 1977, Hourani et al reported the incidence of HBsAg. in urine was about 52% in the patients positive for serum HBsAg. with hemodialysis treatment due to chronic renal failure. A series of studies on the HBsAg. in urine has revealed the urine of the patients positive for serum HBsAg. to be important source of infection. But there's much room to debate on the relationship of HBsAg. in urine with infectivity and the exact mechanism of urinary emergence of HBsAg. The authors detected HBsAg. in serum and urine by employing sandwitch solid-phase radioimmunoassay, and performed urinalysis, liver function test and renal function evaluation. Percutanous liver and/or kidney biopsies were done. Among 38 renal disease patients, 9 cases(23.4%) were shown to be positive for serum HBsAg. and 5 cases(55.5%) among above 9 patients positive for urine HBsAg. 56 cases(67.4%) of 83 liver disease patients revealed positive for serum HBsAg. All 10 renal and liver disease patients revealed positive serum HBsAg., and among the 9 cases(90%) positive for urine HBsAg. In the 25 patients positive for urine HBsAg. all of 5 renal patients and 9 renal and liver patients had hematuria or/and proteinuria above 2 positive for albumin. But in the 11 liver patients 6 cases(55.1%) were normal findings. And there's no significant in cpm of urine HBsAg. between the patient positive for serum HBsAg. and negative, and in cpm of serum HBsAg. between liver and renal disease patients. But there's statistical significance in cm of urine HBsAg. between renal and liver diseases.

  1. Prevalencia de anticuerpos antifosfolípidos en pacientes con conectivopatías

    Directory of Open Access Journals (Sweden)

    Óscar Emilio Ruiz-Franco

    2011-07-01

    Full Text Available Objetivos: Determinar la prevalencia de anticuerpos antifosfolípidos en pacientes con conectivopatías. Diseño: Estudio descriptivo transversal. Institución: Hospital Dos de Mayo e Instituto de Investigaciones Clínicas de la UNMSM, Lima, Perú. Participantes: Pacientes con diagnóstico de colagenopatía y sujetos sanos. Intervenciones: En 100 pacientes con diagnóstico de colagenopatía y 50 sujetos sanos, todos provenientes de Lima Metropolitana y atendidos entre enero y noviembre de 2008, se determinó fibrinógeno, tiempo de protrombina, tiempo parcial de tromboplastina activada, anticuerpos anticardiolipina y anticoagulante lúpico. Principales medidas de resultados: Presencia de anticuerpos anticardiolipina y anticoagulante lúpico. Resultados: Los pacientes con conectivopatías presentaron anticuerpos antifosfolípidos en 17%, anticoagulante lúpico en 13% y anticardiolipina IgG 4%. Al dividir el grupo por conectivopatías, en los 70 pacientes con lupus eritematoso sistémico 10 resultaron positivos para anticoagulante lúpico y 3 tuvieron anticardiolipina IgG; de los 30 pacientes con artritis reumatoide, 3 tuvieron anticoagulante lúpico positivo y 1 presentó anticardiolipina IgG. En el grupo de 50 personas sanas, solo se encontró un caso positivo para anticoagulante lúpico. Conclusiones: La prevalencia de anticuerpos antifosfolípidos en pacientes con conectivopatías fue 17%. La conectivopatía estudiada más frecuente fue el lupus eritematoso sistémico. El anticuerpo antifosfolípido más frecuente fue el anticoagulante lúpico.

  2. Quantitative HBsAg and HBeAg predict hepatitis B seroconversion after initiation of HAART in HIV-HBV coinfected individuals.

    Directory of Open Access Journals (Sweden)

    Gail V Matthews

    Full Text Available OBJECTIVE: Anti-HBe seroconversion and HBsAg loss are important therapeutic endpoints in patients with hepatitis B virus (HBV infection. Quantitative measures of hepatitis B surface antigen (qHBsAg and e antigen (qHBeAg have been identified as potentially useful indicators of therapeutic response in HBV monoinfection. The aim of this study was to examine serological change including quantitative biomarkers in HIV-HBV coinfected patients initiating HBV active antiretroviral therapy (ART. METHODS: HIV-HBV coinfected individuals from Thailand were followed for up to 168 weeks post ART. Rates and associations of qualitative serological change were determined. Longitudinal changes in qHBsAg and qHBeAg were measured and their utility as predictors of response examined. RESULTS: Forty seven patients were included of whom 27 (57% were HBeAg positive at baseline. Median CD4 count was 48 cells/mm(3. Over a median follow-up of 108 weeks 48% (13/27 lost HBeAg, 12/27 (44% achieved anti-HBe seroconversion and 13% (6/47 HBsAg loss. Anti-HBe seroconversion was associated with higher baseline ALT (p = 0.034, lower qHBsAg (p = 0.015, lower qHBeAg (p = 0.031 and greater HBV DNA decline to week 24 (p = 0.045. Sensitivity and specificity for qHBsAg and qHBeAg decline of >0.5 log at week 12 and >1.0 log at week 24 were high for both anti-HBe seroconversion and HBsAg loss. CONCLUSIONS: Rates of serological change in these HIV-HBV coinfected individuals with advanced immunodeficiency initiating HBV-active ART were high. Baseline and on treatment factors were identified that were associated with a greater likelihood of subsequent anti-HBe seroconversion, including both quantitative HBsAg and HBeAg, suggesting these biomarkers may have utility in this clinical setting.

  3. Manejo de la embarazada con isoinmunización por anticuerpos irregulares

    OpenAIRE

    Fuenzalida C,Javiera; Carvajal C,Jorge A

    2014-01-01

    La isoinmunización eritrocitaria feto-materna se define como la presencia de anticuerpos maternos dirigidos contra antígenos presentes en los glóbulos rojos fetales. Los anticuerpos maternos pueden atravesar la barrera placentaria y provocar hemólisis de los glóbulos rojos fetales produciendo anemia hemolítica e hiperbilirrubinemia, características de la enfermedad hemolítica perinatal (EHP). La principal causa de EHP es la incompatibilidad ABO, seguida de la isoinmunización por RhD; esta últ...

  4. Seguridad post-comercialización de los anticuerpos monoclonales antineoplásicos

    OpenAIRE

    González Iglesias, Verónica

    2015-01-01

    INTRODUCCIÓN: Los primeros anticuerpos monoclonales comercializados en España han sido rituximab, trastuzumab, bevacizumab, cetuximab y panitumumab, en este orden. El estudio de su seguridad en los primeros años postcomercialización es fundamental para definir su relación beneficio/riesgo en las condiciones de uso autorizadas. JUSTIFICACIÓN Y OBJETIVOS: Con el fin de estudiar la seguridad postcomercialización de los anticuerpos monoclonales nos planteamos como objetivos: 1) Analizar las s...

  5. Terapia con anticuerpos monoclonales en Cardiología y Medicina Interna

    OpenAIRE

    Christian David Adams-Sánchez; Gabriel Jaime Tobón-García

    2016-01-01

    La terapia biológica basada en anticuerpos monoclonales, constituyen una nueva herramienta terapéutica, que de la mano del desarrollo de la biología molecular permiten intervenir puntos claves en la etiopatogenia de la enfermedad, permitiendo una terapia personalizada. Se han desarrollado nuevos anticuerpos monoclonales que intervienen en las enfermedades cardiovasculares, por lo que el entendimiento de los principios terapéuticos, los tipos y el mecanismo de acción de estos fármacos es esenc...

  6. Helicobacter pylori, hepatitis viruses A, C, E antibodies and HBsAg-prevalence and associated risk factors in pediatric communities of karachi

    International Nuclear Information System (INIS)

    Aziz, S.; Muzzafar, R.; Hafiz, S.; Abbas, Z.; Zafar, M.N.; Naqvi, S.A.A.; Rizvi, S.A.U.H.

    2007-01-01

    To document the prevalence of Helicobacter pylori (H. pylori), Hepatitis A virus (HAV), Hepatitis C virus (HCV), Hepatitis E virus (HEV) antibodies and Hepatitis B virus surface antigen (HBsAg), in the pediatric age group of low socioeconomic urban communities of Karachi and to identify risk factors associated with these infections. Three hundred and eighty children, ages 5 months to 15 years were investigated. Venous blood samples were collected and questionnaire filled on sociodemographic characteristics (family income, number of dependents in the family, area of living, number of people per room per house, and number of children sharing bed with parents and siblings). Gastrointestinal symptoms were recorded. Anti-HAV IgG (Hepatitis A virus IgG antibody), anti-HCV (Hepatitis C virus antibody), anti-HEV (Hepatitis E antibodies) and HBsAg, were analyzed by enzyme immunoassays (EIAs). Samples were also screened for anti-HIV1/2 (human immunodeficiency virus 1 and 2 antibodies by EIA. IgG antibodies against H. pylori were detected by immunochromatography. A correlation between increasing age and seroconversion was seen for hepatotropic viruses. At 14 years and above,100% of the children were found to be positive for anti-HAV, 26% for anti-HEV, and 1.4%, for anti-HCV while HBsAg was positive in 1.9%. H. pylori infection did not show a significant increase with age. Both anti-HAV and anti-H. pylori were present simultaneously in 30% of the population investigated. With age, increasing number of children acquired antibodies against hepatotropic viruses and H. pylori. Occurrence of HBsAg and anti-HEV at a later age suggests horizontal, rather than vertical transmission. (author)

  7. Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants

    DEFF Research Database (Denmark)

    Schirmbeck, Reinhold; Dikopoulos, Nektarios; Kwissa, Marcin

    2003-01-01

    Processing exogenous hepatitis B surface antigen (HBsAg) of the hepatitis B virus (HBV) generates the K(b)-binding S(208-215) epitope 1; processing endogenous HBsAg generates the K(b)-binding S(190-197) epitope 2. Cross-reactive CD8(+) T cell responses were primed to epitope 1 but not epitope 2...... HBs-tg mice showed reduced antigenemia. Hence, vaccination with natural HBsAg variants from different HBV sero/genotypes can prime cross-reactive, specific CD8(+) T cell immunity that breaks tolerance to HBsAg....

  8. High-Burnup-Structure (HBS): Model Development in MARMOT for HBS Formation and Stability Under Radiation and High Temperature

    Energy Technology Data Exchange (ETDEWEB)

    Ahmed, K. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bai, X. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Zhang, Y. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Biner, B. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-09-01

    A detailed phase field model for the formation of High Burnup Structure (HBS) was developed and implemented in MARMOT. The model treats the HBS formation as an irradiation-induced recrystallization. The model takes into consideration the stored energy associated with dislocations formed under irradiation. The accumulation of radiation damage, hence, increases the system free energy and triggers recrystallization. The increase in the free energy due to the formation of new grain boundaries is offset by the reduction in the free energy by creating dislocation-free grains at the expense of the deformed grains. The model was first used to study the growth of recrystallized flat and circular grains. The model reults were shown to agree well with theorrtical predictions. The case of HBS formation in UO2 was then investigated. It was found that a threshold dislocation density of (or equivalently a threshold burn-up of 33-40 GWd/t) is required for HBS formation at 1200K, which is in good agrrement with theory and experiments. In future studies, the presence of gas bubbles and their effect on the formation and evolution of HBS will be considered.

  9. High-Burnup-Structure (HBS): Model Development in MARMOT for HBS Formation and Stability Under Radiation and High Temperature

    International Nuclear Information System (INIS)

    Ahmed, K.; Bai, X.; Zhang, Y.; Biner, B.

    2016-01-01

    A detailed phase field model for the formation of High Burnup Structure (HBS) was developed and implemented in MARMOT. The model treats the HBS formation as an irradiation-induced recrystallization. The model takes into consideration the stored energy associated with dislocations formed under irradiation. The accumulation of radiation damage, hence, increases the system free energy and triggers recrystallization. The increase in the free energy due to the formation of new grain boundaries is offset by the reduction in the free energy by creating dislocation-free grains at the expense of the deformed grains. The model was first used to study the growth of recrystallized flat and circular grains. The model results were shown to agree well with theoretical predictions. The case of HBS formation in UO2 was then investigated. It was found that a threshold dislocation density of (or equivalently a threshold burn-up of 33-40 GWd/t) is required for HBS formation at 1200K, which is in good agreement with theory and experiments. In future studies, the presence of gas bubbles and their effect on the formation and evolution of HBS will be considered.

  10. Immune responses to HBsAg conjugated to protein D of non-typeable Haemophilus influenzae in mice.

    Directory of Open Access Journals (Sweden)

    Qiudong Su

    Full Text Available Hepatitis B vaccine that contains an aluminum hydroxide adjuvant induces apoptotic death of Hepa 1-6 cells. Difficult-to-degrade chemical additives in vaccines effectively enhance vaccine immunogenicity, but also affect the host tissue. Identification of bio-molecules that are readily degraded and compatible in vivo as an adjuvant is important for vaccine research. The hapten-carrier effect suggests that stimulation of helper T (Th cells by carrier adjuvants is feasible. Protein D (PD of non-typeable Haemophilus influenzae covalently conjugated to some polysaccharide vaccines has been confirmed to convert T-cell independent (TI antigens into T-cell dependent (TD antigens, and elicit strong T-cell responses ultimately. Herein, we would substitube PD for aluminum hydroxide adjuvant in Hepatitis B vaccine.Truncated PD (amino acids 20-364 was expressed in Escherichia coli and purified by (NH42SO4 precipitation and DEAE chromatography. After evaluation of antigenicity by western blotting, PD was covalently conjugated to yeast-derived recombinant HBsAg by cross-linking with glutaraldehyde. Intramuscular immunization with the conjugate induced higher level of HBsAg-specific antibody than did HBsAg alone (p < 0.05, and was comparable to commercial Hepatitis B vaccine. During the surveillance period (days 35-105, anti-HBs titers were hold high. Moreover, the conjugated vaccine enhanced Th1 immune responses, while Th2 responses were also activated and induced an antibody response, as determined by IFN-γ ELISPOT and IgG1/IgG2a ratio assays.Recombinant truncated PD covalently conjugated to HBsAg antigen enhanced the immunogenicity of the antigen in mice simultaneously by humoral and cellular immune response, which would facilitate therapeutic hepatitis B vaccines.

  11. LDL oxidada circulante y anticuerpos contra LDL oxidada según niveles de ácido úrico en mujeres con exceso de peso

    OpenAIRE

    Ruíz-Fernández,Nelina; Espinoza-Zavala,Milagros; González,Julio C.; Leal-Herrera,Ulises; Reigosa-Yaniz,Aldo

    2011-01-01

    Objetivo: Establecer si el aumento de ácido úrico sérico se asocia a niveles más elevados de LDL oxidada (LDLox), anticuerpos contra LDLox (anti LDLox) e índices de oxidación de la LDL, en mujeres con exceso de peso. Método: Estudio transversal que incluyó 114 mujeres con índice de masa corporal > 25 kg/m². Se determinó peso, talla, circunferencia abdominal, presión arterial, glicemia, ácido úrico, perfil lipídico, creatinina, apolipoproteína B (ApoB), LDLox, anti LDLox e insulina. Se estimó ...

  12. Terapia con anticuerpos monoclonales en Cardiología y Medicina Interna

    Directory of Open Access Journals (Sweden)

    Christian David Adams-Sánchez

    2016-07-01

    Full Text Available La terapia biológica basada en anticuerpos monoclonales, constituyen una nueva herramienta terapéutica, que de la mano del desarrollo de la biología molecular permiten intervenir puntos claves en la etiopatogenia de la enfermedad, permitiendo una terapia personalizada. Se han desarrollado nuevos anticuerpos monoclonales que intervienen en las enfermedades cardiovasculares, por lo que el entendimiento de los principios terapéuticos, los tipos y el mecanismo de acción de estos fármacos es esencial para un uso adecuado. En este artículo se discutirá el perfil de los anticuerpos monoclonales en la enfermedad cardiovascular, como los inhibidores de la PCSK-9 y el antídoto del Dabigatran, pasando por las moléculas ya conocidas como el abciximab, el antídoto de la digoxina y la terapia inmunomoduladora en el trasplante cardiaco. Del mismo modo, se evalúa una posible aplicación de los anticuerpos monoclonales usados en reumatología que podrían tener un papel importante en el manejo de las enfermedades cardiovasculares.

  13. Value of the detection of anti-HBc by radioimmunological assay in liver disease

    International Nuclear Information System (INIS)

    Noly, J.C.; Sepetjan, M.; Chevallier, P.; Trepo, C.

    1983-01-01

    Detection of anti-HBc by radioimmunossay was carried out on 1365 sera obtained from 947 liver disease patients. Anti-HBc always confirmed HBV infection in patients with HBs antigenemia. By contrast in the absence of detectable HBs Ag or anti-HBs, anti-HBc often becomes the only serological clue suggesting evidence of recent or ongoing HBV infection. Since anti-HBc may persist for prolonged period of time following acute HB infection only distinction between the IgG and IgM classes of anti-HBc may help to distinguish between persistant or past infection [fr

  14. Species specificity for HBsAg binding protein endonexin II

    NARCIS (Netherlands)

    deBruin, WCC; Leenders, WPJ; Moshage, H; vanHaelst, UJGM

    Background/Aims: Hepatitis B virus displays a distinct species and tissue tropism, Previously we have demonstrated that a human liver plasma membrane protein,vith a molecular weight of approximately 34 kiloDalton specifically binds to HBsAg. This protein was identified as endonexin II, a Ca2+

  15. Combined radioimmunoassay of HBs-antigen and anti-HBs using the Biotest combRIA-Au kit

    International Nuclear Information System (INIS)

    Kselikova, M.; Novak, J.; Urbankova, J.

    1979-01-01

    Hepatitis B antigen and antibody were determined simultaneously by radioimmunoassay using the Biotest combRIA-Au kit. The results in determining antigens and antibodies in sera with a known content of these substances by this kit nearly equal those of isolated determinations of antigen by the Abbott AUSRIA II-125 kit, and of antibody by the Abbott AUSAB kit, resp., except for a negligible number of sera with a very low content of either antigen or antibody. (author)

  16. Application of a Newly Developed High-Sensitivity HBsAg Chemiluminescent Enzyme Immunoassay for Hepatitis B Patients with HBsAg Seroclearance

    OpenAIRE

    Shinkai, Noboru; Matsuura, Kentaro; Sugauchi, Fuminaka; Watanabe, Tsunamasa; Murakami, Shuko; Iio, Etsuko; Ogawa, Shintaro; Nojiri, Shunsuke; Joh, Takashi; Tanaka, Yasuhito

    2013-01-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstr...

  17. Prevalencia de anticuerpos para Trypanosoma cruzi en caninos de dos municipios endémicos de Boyacá

    Directory of Open Access Journals (Sweden)

    Diego Manrique Abril

    2012-04-01

    Full Text Available Objetivo. evaluar la prevalencia de anticuerpos anti Trypanosoma cruzi (T. cruzi en una muestra de caninos domésticos residentes en dos municipios endémicos. Materiales y métodos. Se tomaron muestras séricas de 20 caninos procedentes de hogares donde residen mujeres gestantes seropositivas y 40 perros habitantes de hogares de mujeres gestantes seronegativas en Miraflores y Moniquira, Boyacá. El análisis se realizó mediante prueba diagnóstica rápida dipstick de InBios. Resultados. Se encontró prevalencia del 16.7% en Moniquirá y del 13.3% Miraflores respectivamente con una prevalencia general del 15% en los dos municipios. Se halló riesgo 3 veces mayor de que ocurra la infección en caninos, en los hogares donde residen gestantes seropositivas; además la infestación por pulgas y garrapatas en el animal, hábitat cercano a la vivienda, se relacionan con mayor seropositividad en el canino. Conclusiones. La raza, el sexo, la presencia de aves en la casa y al examen clínico general son considerados factores pronósticos en en la infección por Trypanosoma cruzi en caninos. Como factores protectores se identificó la desparasitación y vacunación de los animales.

  18. Prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre del IMSS, Orizaba, Veracruz, México

    Directory of Open Access Journals (Sweden)

    Ramos-Ligonio Angel

    2006-01-01

    Full Text Available OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70 y un extracto crudo de epimastigotes. Las muestras fueron obtenidas entre los meses de octubre de 2001 a enero de 2002. RESULTADOS: Los 420 donadores de sangre analizados fueron seronegativos para HBV, HCV, BrA, VDRL y HIV. Después del tamizaje de los 420 donadores, se identificaron dos individuos seropositivos por las pruebas de ELISA, Western blot e IFI, con una seroprevalencia de 0.48%. CONCLUSIONES: En este estudio se muestran evidencias de seropositividad para T. cruzi en donadores de sangre del HGRO, lo que sugiere la existencia de riesgo de contaminación por transfusión sanguínea. Por tal motivo, es necesario aplicar programas para el tamizaje serológico a través de técnicas inmunológicas con alta sensibilidad y especificidad.

  19. Papel de los anticuerpos beta2-glicoproteína I circulantes en pacientes con enfermedad arterial periférica en la modulación de un genotipo endotelial pro-aterogénico

    OpenAIRE

    Varela Casariego, César

    2015-01-01

    Tesis inédita presentada en la Universidad Europea de Madrid. Facultad de Ciencias Biomédicas. Programa de Doctorado en Biomedicina y Ciencias de la Salud La enfermedad arterial periférica (EAP) es una enfermedad arterial inflamatoria crónica que se inicia con la pérdida de las funciones homeostáticas del endotelio. Esta disfunción endotelial se ha asociado con una disminución de la bioactividad del óxido nítrico (NO). En este contexto, los anticuerpos anti-beta2-glicoproteína I (ABGPI) se...

  20. PCSK-9: papel en las hipercolesterolemias y anticuerpos monoclonales específicos inhibitorios

    Directory of Open Access Journals (Sweden)

    Álvaro J. Ruiz

    2017-11-01

    Los anticuerpos monoclonales contra PCSK9, evolocumab y alirocumab, son una novedosa herramienta terapéutica de gran utilidad en los casos de hipercolesterolemia familiar heterocigota, en algunos casos de hipercolesterolemia familiar homocigota y de personas con alto riesgo cardiovascular, cuyos niveles de cLDL no pueden ser controlados con dosis máximas toleradas de las estatinas de mayor efectividad, además de ezetimibe y cambios en el estilo de vida.

  1. Papel de los anticuerpos en la protección contra micobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Nesty Olivares

    2006-12-01

    Full Text Available Convencionalmente se asume que la defensa del hospedero contra Mycobacterium tuberculosis se basa en los mecanismos de inmunidad celular exclusivamente y se descarta el papel de los anticuerpos en la protección. En este trabajo se analizan evidencias recientes que retan este dogma y sugieren la importancia de considerar la manipulación de la respuesta inmune humoral como una alternativa en la investigación de vacunas contra la tuberculosis.

  2. Serologic tracers (HBsAg and HBsAc) of hepatitis B virus in expectant mothers of the Perinatal Maternal Institute

    CERN Document Server

    Garcia, B M

    1997-01-01

    A study on hepatitis B tracers, (HBsAg and HBsAc), was conducted in women with different months of pregnancy at the Perinatal Maternal Institute in Lima, Peru. A total of 1010 mothers were studied during the period of January to October 1996, establishing by radioimmunoassay (RIA) whether they were positive or not. The results showed a prevalence rate of 1,6 for HBsAc and 1,3 for HBsAg for every 100,000 inhabitants. The incidence rate was 0,332 for HBsAc and 0,07 for HBsAg for every 100,000 inhabitants. This means that 21 expectant mothers are HBsAc positive and 5 are HBsAg positive. According to the investigations, there were different ways of transmission; promiscuity must be highlighted, as well as age -most of the mothers who were positive were between 20 and 25 years old- and origin. Most of them were immigrants from different places and live in shantytowns, which indicates that most of them have limited economic resources and have not received any orientation on family planning.

  3. Serologic tracers (HBsAg and HBsAc) of hepatitis B virus in expectant mothers of the Perinatal Maternal Institute

    International Nuclear Information System (INIS)

    Garcia G, B.M.

    1997-01-01

    A study on hepatitis B tracers, (HBsAg and HBsAc), was conducted in women with different months of pregnancy at the Perinatal Maternal Institute in Lima, Peru. A total of 1010 mothers were studied during the period of January to October 1996, establishing by radioimmunoassay (RIA) whether they were positive or not. The results showed a prevalence rate of 1,6 for HBsAc and 1,3 for HBsAg for every 100,000 inhabitants. The incidence rate was 0,332 for HBsAc and 0,07 for HBsAg for every 100,000 inhabitants. This means that 21 expectant mothers are HBsAc positive and 5 are HBsAg positive. According to the investigations, there were different ways of transmission; promiscuity must be highlighted, as well as age -most of the mothers who were positive were between 20 and 25 years old- and origin. Most of them were immigrants from different places and live in shantytowns, which indicates that most of them have limited economic resources and have not received any orientation on family planning

  4. Occult Hepatitis B Virus Infection and Associated Genotypes among HBsAg-negative Subjects in Burkina Faso

    Science.gov (United States)

    Diarra, Birama; Yonli, Albert Théophane; Sorgho, Pegdwendé Abel; Compaore, Tegwindé Rebeca; Ouattara, Abdoul Karim; Zongo, Wendpagnangdé Arsène; Tao, Issoufou; Traore, Lassina; Soubeiga, Serge Théophile; Djigma, Florencia Wendkuuni; Obiri-Yeboah, Dorcas; Nagalo, Bolni-Marius; Pietra, Virginio; Sanogo, Rokia; Simpore, Jacques

    2018-01-01

    Background The presence of HBV DNA in the liver (with detectable or undetectable HBV DNA in the serum) of individuals tested HBsAg negative by currently available assays is defined occult B Infection (OBI). It remains a potential transmission threat and risk to HBV chronic infection. The purpose of this study was to determine the OBI prevalence among HBsAg negative subjects and to characterize associated genotypes. Methods Blood samples of 219 HBsAg-negative subjects tested by ELISA were collected. HBV DNA was investigated in all samples. Viral loads were determined using quantitative real-time PCR. All samples were screened for HBV markers (anti-HBc, anti-HBe, HBsAg). The Pre-S/S region of the HBV genome was sequenced. The database was analyzed using the SPSS and Epi info software. Phylogenetic analysis was performed using the BioEdit and MEGA software. Results Of the 219 samples, 20.1% were anti-HBc positive, 1.8% HBeAg and 22.8% were anti-HBe positive. Fifty-six (56) (25.6%) of the samples had a detectable HBV DNA and viral loads ranging from 4 IU/mL to 13.6 106 IU/mL. Sixteen of them (16/56) had a viral load 200 IU/mL, resulting in a “false OBI” prevalence of 18.3% (40/219). HBV genotype E was predominant followed by the quasi-sub-genotype A3. A single “false OBI” strain had the characteristic mutation G145R. Other mutations were observed and all located in the major hydrophilic region (MHR) of the S gene. Conclusion The study reported a prevalence of 7.3% of occult hepatitis B infection. It confirms the predominance of genotype E and the existence of a subgroup of quasi-sub-genotype A3 of HBV in Burkina Faso. It further provides information on the presence of “false OBI.” This study has found mutations in the major hydrophilic region (MHR) of the pre-S/S gene of HBV. PMID:29326804

  5. Comparison of serum HBsAg quantitation by four immunoassays, and relationships of HBsAg level with HBV replication and HBV genotypes.

    Directory of Open Access Journals (Sweden)

    Edouard Tuaillon

    Full Text Available BACKGROUND: The decline in hepatitis B virus surface antigen (HBsAg may be an early predictor of the viral efficacy of Hepatitis B virus (HBV therapy. The HBsAg levels obtained by different immunoassays now need comparing and the relationships between levels of HBsAg and HBV DNA alongside HBsAg and genotype must be evaluated. METHODOLOGY/PRINCIPAL FINDINGS: HBsAg levels were compared among 80 patients using the Abbott Architect assay, a commercial immunoassay approved for HBsAg detection and quantitation, and three other assays derived from immunoassays approved for HBsAg detection (manufactured by Diasorin, Bio-Rad and Roche. Good correlation was found between the Abbot vs. Diasorin, Bio-Rad and Roche assays with narrow 95% limits of agreement and small mean differences: -0.06 to 0.11, -0.09 log(10 IU/mL; -0.57 to 0.64, -0.04 log(10 IU/mL; -0.09 to 0.45, -0.27 log(10 IU/mL, respectively. These agreements were not affected by genotypes A or D. HBsAg was weakly correlated with HBV DNA, whatever the HBsAg assay used: Abbott, ρ = 0.36 p = 0.001, Diasorin ρ = 0.34, p = 0.002; Bio-Rad ρ = 0.37, p<0.001; or Roche ρ = 0.41, p<0.001. This relationship between levels of HBsAg and HBV DNA seemed to depend on genotypes. Whereas HBsAg (Abbott assay tended to correlate with HBV DNA for genotype A (ρ = 0.44, p = 0.02, no such correlation was significant for genotypes D (ρ = 0.29, p = 0.15. CONCLUSION/SIGNIFICANCE: The quantitation of HBsAg in routine clinical samples is comparable between the reference assay and the adapted assays with acceptable accuracy limits, low levels of variability and minimum discrepancy. While HBsAg quantitation is not affected by HBV genotype, the observed association between levels of HBsAg and HBV DNA seems genotype dependent.

  6. Concentración de anticuerpos contra proteínas de las glándulas salivales de Aedes aegypti e historia de la exposición al virus del dengue en residentes de una zona endémica colombiana

    Directory of Open Access Journals (Sweden)

    Berlin Londoño-Rentería

    2015-12-01

    Full Text Available Introducción. Las proteínas salivales de los mosquitos son capaces de inducir la producción de anticuerpos, lo que a su vez refleja el grado de contacto hombre-vector. Además, los anticuerpos IgG contra el virus del dengue son indicadores de una exposición previa a este virus. El riesgo de transmisión del virus del dengue está asociado no solo con factores relacionados con la biología del mosquito, o factores virales, sino también, con factores socioeconómicos, como la disponibilidad de agua en el hogar, que pueden desempeñar un papel importante durante la temporada epidémica. Objetivo. Determinar el efecto de la presencia de mosquitos Aedes aegypti en las casas y la exposición previa al virus del dengue, sobre los niveles de anticuerpos contra mosquitos en el contacto humano-vector en habitantes de un área endémica de Colombia. Materiales y métodos. Se hizo un estudio piloto de 58 casas y 55 participantes en Norte de Santander, Colombia. Se empleó un cuestionario para recopilar la información sobre los factores socioeconómicos y se examinaron las casas para detectar la presencia de sitios de cría de Ae. aegypti. Se recolectó una muestra de sangre humana total en papel de filtro y se estableció el nivel de anticuerpos IgG contra el virus del dengue, además del de los anticuerpos IgG e IgM anti-Ae. aegypti de extracto de glándula salival mediante ELISA. Resultados. Los resultados revelaron un mayor nivel de anticuerpos IgG de extracto de glándula salival en sujetos que vivían en casas con presencia de mosquitos Ae. aegypti en la fase acuática. Asimismo, se encontró una mayor concentración de anticuerpos IgG de extracto de glándula salival en personas previamente expuestas al virus del dengue. Los resultados evidenciaron una correlación positiva significativa entre los niveles de IgM de extracto de glándula salival y los de IgG anti-virus del dengue de extracto de glándula salival, y una correlación negativa con

  7. [Comparative study between HBsAg positive and HBsAg negative panarteritis nodosa in a series of 25 cases (author's transl)].

    Science.gov (United States)

    Iturralde, J F; Miranda, C F; Barbado, J; Peña, J; Seoane, J; Gil, A; Arnalich, F; Vázquez, J J

    1980-06-25

    Clinical symptoms, biochemical analyses, immunologic status and angiographic findings corresponding to seven HBsAg positive patients with panarteritis nodosa and to 16 HBsAg negative patients with panarteritis nodosa have been compared. HBsAg positive cases showed a statistical significant higher incidences of Raynaud's phenomenon (p less than 0.05) and cardiopathies (p les than 0.05), as well as high occurrence of blood hypertension, artropathy, liver involvement and peripheral neuropathy. Significant differences in relation to cell immunity were not found. Humoral immune disturbances were more common among HBsAg positive patients, being hypergammaglobulinemia (p less than 0.01), IgM increase and decrease of complement factors (C3, C4 and C3PA) the most frequent abnormalities recorded. Angiographic studies revealed a high incidence of microaneurisms for the HBsAg positive group.

  8. The effects of booster vaccination of hepatitis B vaccine on anti-HBV surface antigen negative children 11-15 years after primary vaccination.

    Science.gov (United States)

    Yao, Jun; Ren, Jingjing; Shen, Lingzhi; Chen, Yongdi; Liang, Xiaofeng; Cui, Fuqiang; Li, Qian; Jiang, Zhenggang; Wang, Fuzhen

    2011-10-01

    The twin aims of this study were to investigate the changes in anti-HBs IgG levels after booster vaccinations and to compare the effects of different vaccine doses in children aged 11-15 years who were both negative for HBsAg and had an Anti-HBs vaccination. Children who were born between 1993 and 1998 and who had completed their Hepatitis B vaccination program in infancy were randomly recruited to the study. The participants were divided into three groups according to their anti-HBs IgG levels: group I had a level vaccination program comprised three (20μg) doses of HepB (CHO) vaccine administered at zero, one and six months after they are join this program: anti-HBs levels were measured one month after the first and third vaccinations. Among 448 HBsAg-negative infants, anti-HBs seroconversion rates (defined as an anti-HBs >= 10 mIU/mL) after the first and third vaccinations were 85.5% and 98.6% respectively - these observed differences were statistically significant (χ2 [1dof] = 50.11, pnegative (anti-HBsanti-HBs titer levels decay to 10mIU/ml in 11-15 years of age children completed HepB Basic immunization, which need for booster immunization. The effect is better for those children with a relatively higher antibody titer before booster, and the effect of three doses booster is best.

  9. Hepatitis B Reactivation in a HBsAg-Negative, HBcAb-Positive Patient Receiving Fludarabine for the Treatment of Chronic Lymphocytic Leukaemia

    Directory of Open Access Journals (Sweden)

    Federica Toscanini

    2011-01-01

    Full Text Available Hepatitis B virus (HBV reactivation is an increasingly recognized cause of morbidity and mortality in patients undergoing chemotherapy. In haematology, the risk of reactivation of B hepatitis among HBsAg-positive patients has been documented; therefore, use of lamivudine prophylaxis is recommended before starting chemotherapy. Differently, for HBsAg-negative patients with markers of previous HBV infection (i.e., presence of isolated anti-HBc positivity (anticore patients management strategies are not univocal. We describe a rare case of HBV reactivation in an anticore patient after fludarabine therapy for chronic lymphocytic leukaemia. The patient fully recovered after a 6-month course of lamivudine with persistent HBV-DNA clearance and loss of HBsAg. The most important feature of this case is that fludarabine alone infrequently determines HBV reactivation, especially in anticore patients. Therefore, we suggest that patients candidates to receive fludarabine therapy should be considered for lamivudine prophylaxis, not only if HBsAg-positive, but even if anticore-positive only.

  10. Hepatitis B Reactivation in a HBsAg-Negative, HBcAb-Positive Patient Receiving Fludarabine for the Treatment of Chronic Lymphocytic Leukaemia.

    Science.gov (United States)

    Toscanini, Federica; De Leo, Pasqualina; Calcagno, Giuseppe; Malfatti, Federica; Grasso, Alessandro; Anselmo, Marco

    2011-01-01

    Hepatitis B virus (HBV) reactivation is an increasingly recognized cause of morbidity and mortality in patients undergoing chemotherapy. In haematology, the risk of reactivation of B hepatitis among HBsAg-positive patients has been documented; therefore, use of lamivudine prophylaxis is recommended before starting chemotherapy. Differently, for HBsAg-negative patients with markers of previous HBV infection (i.e., presence of isolated anti-HBc positivity) (anticore patients) management strategies are not univocal. We describe a rare case of HBV reactivation in an anticore patient after fludarabine therapy for chronic lymphocytic leukaemia. The patient fully recovered after a 6-month course of lamivudine with persistent HBV-DNA clearance and loss of HBsAg. The most important feature of this case is that fludarabine alone infrequently determines HBV reactivation, especially in anticore patients. Therefore, we suggest that patients candidates to receive fludarabine therapy should be considered for lamivudine prophylaxis, not only if HBsAg-positive, but even if anticore-positive only.

  11. Espectro de enfermedades neurológicas asociadas a anticuerpos anti-glutamato decarboxilasa (GAD)

    OpenAIRE

    Saiz Hinajeros, Albert

    1998-01-01

    El GAD (glutamato decarboxilasa) es la enzima que cataliza la síntesis del neurotransmisor inhibitorio ácido gamma-aminobutírico (GABA) a partir del glutamato. El GAD se expresa selectivamente en los botones sinápticos de las neuronas GABA-érgicas. Fuera del sistema nervioso se encuentra en altas concentraciones en las células beta de los islotes pancreáticos, sin que existan diferencias entre el GAD de las células pancreáticas o del sistema nervioso.La repuesta inmune contra el GAD se ha rel...

  12. Detection of HBs antigens and antibodies by immunoenzymology and radioimmunology. Comparison of the results

    International Nuclear Information System (INIS)

    Fauchet, R.; Genetet, B.; Phengsavath

    1981-01-01

    The respective sensitivity threshold of immunoenzymology and radioimmunology in HBs antigen and antibody detection were compared and the optical density (o.d.) and counts per minute (cpm) values were correlated with the concentration in ng/ml of a given sample. The sensitivity comparison between RIA and EIA appears in favour of the former technique for both HBs antigen and HBs antibody detection. However the procedure tried here to detect HBs antibodies by immunoenzymology is simple, requires only the choice of neutralising antigenic pool and sensitivity threshold and could usefully be generalized for teams not authorized to handle radioelements. Quantification of the HBs antigen content in ng/ml is a difficult operation, needing great care in the dilution of the sera tested. The reproducibility is not perfect. The semi-quantitative RIA determination expressed in R.U. (radioimmunological units) seems adequate as a mean to follow the progress of an HBs antibody in both liver disease and vaccination [fr

  13. Construction and characterization of calreticulin-HBsAg fusion gene recombinant adenovirus expression vector.

    Science.gov (United States)

    Ma, Chun-Ling; Wang, Gui-Bin; Gu, Run-Guo; Wang, Fang

    2010-06-28

    To generate recombinant adenoviral vector containing calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine. CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease digestion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5' end. Adenoviral expression vector containing CRT-HBsAg fusion gene was constructed by homologous recombinantion. The human embryo kidney (HEK) 293A cells were transfected with linearized DNA plasmid of the recombinant adenoviral vector to package and amplify recombinant adenovirus. The recombinant adenovirus titer was characterized using the end-dilution assay. The expression of the CRT/HBsAg fusion protein in Ad-CRT/HBsAg infected 293A cells was detected by Western blotting. The CRT-HBsAg fusion gene was characterized by PCR and sequencing and its length and sequence were confirmed to be accurate. The CRT-HBsAg fusion gene recombinant pENTR/D-TOPO transfer vector was constructed. The recombinant adenoviral vector, Ad-CRT/HBsAg, was generated successfully. The titer of Ad-CRT/HBsAg was characterized as 3.9 x 10(11) pfu/mL. The CRT-HBsAg fusion protein was expressed by HEK 293A cells correctly. CRT/HBsAg fusion gene recombinant replication-defective adenovirus expression vector is constructed successfully and this study has provided an experimental basis for further studies of Hepatitis B virus gene therapy.

  14. Prevalencia de anticuerpos neutralizantes contra los serotipos del virus dengue en universitarios de Tabasco, México Prevalence of neutralizing antibodies to dengue virus serotypes in university students from Tabasco, Mexico

    Directory of Open Access Journals (Sweden)

    Gilma Guadalupe Sánchez-Burgos

    2008-10-01

    Full Text Available OBJETIVO: Determinar la seroprevalencia de anticuerpos neutralizantes de los serotipos del virus dengue en estudiantes universitarios de Tabasco, México, durante los meses de septiembre a noviembre del año 2005. MATERIAL Y MÉTODOS: Se determinó la presencia de IgG contra el virus en el suero de estudiantes que acudieron al centro clínico de la universidad; en los sueros positivos se determinaron los anticuerpos neutralizantes mediante el ensayo de reducción de placa lítica. RESULTADOS: La prevalencia de IgG contra el dengue fue de 9.1%; de esta proporción, los anticuerpos neutralizantes fueron DENV-1 (20%, DENV-2 (100%, DENV-3 (4% y DENV-4 (68%. CONCLUSIONES: Este estudio muestra que el serotipo transmitido con mayor frecuencia en el estado de Tabasco es el DENV-2, aunque no ha sido el aislado con más frecuencia. La elevada prevalencia de anticuerpos neutralizantes contra el DENV-4, al parecer de reacción cruzada, podría explicar la baja circulación de este serotipo en Tabasco.OBJECTIVE: Determine the seroprevalence of neutralizing antibodies to dengue virus in students from the state university of Tabasco, Mexico. MATERIAL AND METHODS: A transversal study was conducted of serum collected from students between September and November, 2005. The sera were screened for anti-dengue IgG and those that had evidence of dengue antibodies were analyzed by a plaque reduction neutralization test. RESULTS: Prevalence of anti-dengue IgG was 9.1%. The frequency of neutralizing antibodies was 100% for DENV-2, 68% for DENV-4, 20% for DENV-1, and 4 % for DENV-3. CONCLUSIONS: We found that in this population, DENV-2 circulates more than DENV-3 despite the fact that DENV-3 is more frequently isolated. Unexpectedly, neutralizing antibodies against DENV-4 were frequently found even though this serotype is almost extinct; thus, it is probable that cross-immunity could suppress DEN-4 transmission, as has been suggested.

  15. Obtención de anticuerpos policlonales IgY antiparvovirus canino y evaluación en un sistema de aglutinación con látex

    Directory of Open Access Journals (Sweden)

    Jannet González-Figueredo

    2015-12-01

    Full Text Available La parvovirosis canina es una de las principales infecciones que provoca gastroenteritis, fundamentalmente en cachorros, con altos índices de morbiletalidad. Los diagnosticadores más utilizados se basan en la detección de partículas virales excretadas durante la fase aguda de la enfermedad. Algunos requieren equipos especializados, lo que aumenta los costos y el tiempo de diagnóstico. Por esto, la simplificación de estos métodos con alta sensibilidad y especificidad es prioritaria. En Cuba solo se logra un diagnóstico presuntivo sin la completa confirmación de la enfermedad, principalmente por la escasez o ausencia de un medio diagnóstico en toda la red de consultorios y clínicas veterinarias, rápido, eficaz y ajustable a nuestras condiciones. Este trabajo tuvo como objetivo obtener anticuerpos policlonales IgY a partir de yema de huevo de gallina y evaluarlos mediante un sistema de látex-aglutinación, para su posible uso como terapia y principalmente en el diagnóstico. Se inmunizaron por vía intramuscular dos gallinas de raza Leghorn con una cepa atenuada de parvovirus canino (PVC tipo 2. Se aplicaron 8 inoculaciones por ave cada 15 días. Se cosecharon los huevos y se purificaron los anticuerpos por el método de sulfato de dextrana/sulfato de sodio. Se determinó el título de IgY anti-PVC por el método de Inhibición de la Hemoaglutinación (IH y con antígeno de PVC acoplado a partículas de látex de poliestireno. Por IH se obtuvo un título de IgY anti-PVC de 1:1024. Las mezclas de anticuerpos con títulos alto y medio de ambas gallinas aglutinaron con los reactivos preparados con 230 y 460 µg/mL de antígeno. La mayor intensidad de la reacción y la mejor detectabilidad correspondieron a los reactivos elaborados con los anticuerpos de mayor título.

  16. Clinical characteristics of patients with HBsAg(+ versus HBsAg(-/HBcAb(+ primary hepatic carcinoma treated by hepatectomy: a comparative analysis

    Directory of Open Access Journals (Sweden)

    YANG Keli

    2015-06-01

    Full Text Available ObjectiveTo explore the differences in clinical characteristics between patients with HBsAg(+ and HBsAg(-/HBcAb(+ primary hepatic carcinoma (PHC treated by hepatectomy. MethodsForty-three HBsAg(+ and 18 HBsAg(-/HBcAb(+ patients who underwent liver resection against PHC from October 2009 to November 2014 in Guangzhou 8th People′s Hospital were selected for the study. The clinical data of the subjects, including sex, age, histological differentiation, intravascular tumor thrombi, and hepatic cirrhosis, were compared, using t test for continuous data, chi-square test for categorical data, and Mann-Whitney U test for non-parametric data. ResultsNo significant differences existed between patients with HBsAg(+ and HBsAg(-/HBcAb(+ PHC in terms of the age of onset (50.77±12.93 years vs 54.28±9.89 years, t=-1.031, P>0.05, the incidence of cholangiocarcinoma (2.3% vs 167%, χ2=2.24, P>0.05, the incidence of hepatic cirrhosis (62.8% vs 44.4%, χ2=1.746, P>0.05, alpha-fetoprotein level (3638±7869 ng/ml vs 3577±9628 ng/ml, t=0.026, P>0.05, histological differentiation (Z=-1.085, P>0.05, and the rate of intravascular tumor thrombi (34.9% vs 22.2%, χ2=0.949, P>0.05. ConclusionThere are no significant differences in the age of onset and progression of disease between patients with HBsAg(+ and HBsAg(-/HBcAb(+ PHC treated by hepatectomy. However, given the possibility of occult hepatitis B virus infection, it is necessary to monitor hepatic carcinoma even post HBsAg seroconversion

  17. Prediction of the HBS width and Xe concentration in grain matrix by the INFRA code

    International Nuclear Information System (INIS)

    Yang, Yong Sik; Lee, Chan Bok; Kim, Dae Ho; Kim, Young Min

    2004-01-01

    Formation of a HBS(High Burnup Structure) is an important phenomenon for the high burnup fuel performance and safety. For the prediction of the HBS(so called 'rim microstructure') proposed rim microstructure formation model, which is a function of the fuel temperature, grain size and fission rate, was inserted into the high burnup fuel performance code INFRA. During the past decades, various examinations have been performed to find the HBS formation mechanism and define HBS characteristics. In the HBEP(High Burnup Effects Program), several rods were examined by EPMA analysis to measure HBS width and these results were re-measured by improved technology including XRF and detail microstructure examination. Recently, very high burnup(∼100MWd/kgU) fuel examination results were reported by Manzel et al., and EPMA analysis results have been released. Using the measured EPMA analysis data, HBS formation prediction model of INFRA code are verified. HBS width prediction results are compared with measured ones and Xe concentration profile is compared with measured EPMA data. Calculated HBS width shows good agreement with measured data in a reasonable error range. Though, there are some difference in transition region and central region due to model limitation and fission gas release prediction error respectively, however, predicted Xe concentration in the fully developed HBS region shows a good agreement with the measured data. (Author)

  18. Presencia de anticuerpos IgG del virus de la rubéola, virus herpes simple y citomegalovirus en embarazadas residentes en Ciudad de La Habana

    Directory of Open Access Journals (Sweden)

    Carmen Acosta

    2001-06-01

    Full Text Available Se realizó un estudio seroepidemiológico con 207 gestantes, residentes en el municipio Marianao de Ciudad de la Habana, con la finalidad de conocer la presencia de anticuerpos IgG anti-rubéola, anti-citomegalovirus y anti-herpes simple (1 y 2; el nivel de positividad se expresó como la relación de la fluorescencia de la muestra con respecto a la del control positivo, para un nivel de corte de 0,3 para rubéola, 0,2 para citomegalovirus y 0,185 para herpes simple. Las seroconversiones se expresaron como el incremento de esta relación con respecto a la determinación realizada en el primer trimestre de embarazo. El 91,3% de las gestantes resultaron positivas para rubéola, el 92,7% para citomegalovirus y el 98,1% para herpes simple. Se sugiere la valoración de aspectos fundamentales relacionados con estas infecciones dentro del Programa Nacional de Atención a las Embarazadas.

  19. OBTENCIÓN DE ANTICUERPOS POLICLONALES IgY ANTIPARVOVIRUS CANINO A PARTIR DE YEMA DE HUEVO DE GALLINA

    OpenAIRE

    Pinto, J.; Unidad de Investigaciones Agropecuarias (UNIDIA) Microbiología Agrícola y Veterinaria Facltad de Ciencias Pontificia Universidad Javeriana, Bogotá; Barco, M.; Unidad de Investigaciones Agropecuarias (UNIDIA) Microbiología Agrícola y Veterinaria Facltad de Ciencias Pontificia Universidad Javeriana, Bogotá; Afanador, M. C.; Unidad de Investigaciones Agropecuarias (UNIDIA) Microbiología Agrícola y Veterinaria Facltad de Ciencias Pontificia Universidad Javeriana, Bogotá; Merchán, A. M.; Unidad de Investigaciones Agropecuarias (UNIDIA) Microbiología Agrícola y Veterinaria Facltad de Ciencias Pontificia Universidad Javeriana, Bogotá; Montañez, M. F.; Purificación y Análisis de Fluidos Ltda. Bogotá,; Andrade, F.; Purificación y Análisis de Fluidos Ltda. Bogotá.; Torres, O.; Unidad de Investigaciones Agropecuarias (UNIDIA) Microbiología Agrícola y Veterinaria Facltad de Ciencias Pontificia Universidad Javeriana, Bogotá

    2005-01-01

    Inmunizando un grupo de diez gallinas de postura raza Lohmann de 16 semanas de edad con parvovirus canino (CPV) cepa vacunal, se obtuvieron anticuerpos IgY policlonales contra CPV en yema de huevo.En la extracción de los anticuerpos de la yema se requirieron dos pasos, el primero para la remoción de lípidos y el segundo para la precipitación de proteínas. Para la remoción de lípidos se usó el método PBS:Cloroformo y para la precipitación de los anticuerpos solubles (Inmunoglobulinas-IgY) se u...

  20. Asociación entre enfermedad cardiovascular y anticuerpos contra Chlamydia pneumoniae

    Directory of Open Access Journals (Sweden)

    García-Elorriaga Guadalupe de los A

    2002-01-01

    Full Text Available Objetivo. Precisar si existe asociación entre enfermedad cardiovascular (ECV y anticuerpos contra Chlamydia en población mexicana. Material y métodos. Estudio transversal, realizado en la Unidad de Investigación en Inmunología e Infectología -Hospital de Infectología del Centro Médico Nacional La Raza (CMNR- y en el Servicio de Cirugía Cardiovascular y Asistencia Circulatoria, del Hospital General del CMNR, Instituto Mexicano del Seguro Social (IMSS, de agosto de 1998 a abril de 2000. Se determinaron anticuerpos IgG e IgM contra C. psittaci, C. trachomatis y C. pneumoniae mediante microinmunofluorescencia, en suero de 70 pacientes con ECV hospitalizados en el CMNR, mayores de 30 años, de uno u otro sexo, y se compararon con 140 sanos, pareados por edad y sexo. Se utilizaron muestras aleatorias simples, con un tamaño poblacional de 110, una prevalencia de 50% y un nivel de confianza de 99%. Para establecer la diferencia entre las proporciones de los títulos se utilizó ji cuadrada y se calculó la razón de momios. Resultados. El 94.3% (66/70 de los pacientes presentó IgG en contra de C. pneumoniae vs 37% (52/140 de los individuos sanos (p<0.001. Conclusiones. Existe una fuerte asociación entre anticuerpos IgG hacia C. pneumoniae y ECV.

  1. Comparison of hepatitis E virus seroprevalence between HBsAg-positive population and healthy controls in Shandong province, China.

    Science.gov (United States)

    Zhang, Li; Jiang, Zechun; Lv, Jingjing; Liu, Jiaye; Yan, Bingyu; Feng, Yi; Li, Li; Zhang, Guomin; Wang, Fuzhen; Xu, Aiqiang

    2018-02-12

    Persons with chronic hepatitis B (CHB) infection were reported to suffer severe disease after hepatitis E virus (HEV) superinfection, but the studies regarding HEV seroprevalence in this population were limited. A recent study in Vietnam found higher HEV seroprevalence among CHB patients compared with healthy controls. A community-based case-control study was conducted in two counties of Shandong province, China, where hepatitis E incidence was at the highest (Rushan) and lowest (Zhangqiu) in the province based on data from routine public health surveillance. Four townships were selected randomly from each county and all residents in these townships were tested for hepatitis B surface antigen (HBsAg). Those tested positive for HBsAg (CHB group) and the 1:1 age and sex-matched HBsAg-negative residents (control group) were included. Anti-HEV IgM and IgG were tested and positive rates of IgG and IgM were compared between the CHB group and the control group. In total, 2048 CHB participants and 2054 controls were included in the study. In the CHB group, HEV IgG seroprevalence was 9.16% (95% CI: 7.47-11.09) in Zhangqiue and 38.06% (95% CI: 35.07-41.19) in Rushan (P < 0.001); the corresponding rates of IgM were 0.1% (95% CI: 0.002-0.54) and 1.57% (95% CI: 0.90-2.53), respectively (P < 0.001). HEV IgG seroprevalence was similar between CHB group and the control group in both counties (P = 0.21, P = 0.47, respectively) and the same results were found for the positive rate of IgM (P = 0.103, P = 0.262, respectively). Multivariable analysis showed the status of HBsAg was not independently associated with the status of anti-HEV IgG in either Zhangqiu or Rushan [P = 0.187, OR = 1.23(95% CI: 0.90, 1.68); P = 0.609, OR = 1.05 (95% CI: 0.87, 1.26)]. The seroprevalence of HEV varies greatly in different geographic areas, but the seroprevalence is similar between populations with and without CHB. CHB patients residing in high HEV endemic areas

  2. ¿Sabía usted? Anticuerpos antifosfolípidos

    Directory of Open Access Journals (Sweden)

    Javier Molina

    1992-02-01

    Full Text Available

    Los anticuerpos antifosfolípidos comprenden varias poblaciones de inmunoglobulinas, que pueden ocasionar falsos positivos biológicos con el VDRL, interferir con el complejo activador de la protrombina (anticoagulante lúpico o reaccionar con elfosfolípido cardiolipina en una prueba de enzimoinmunoanálisis (EIA.

  3. Anticuerpos antifosfolípido en el lupus eritematoso sistémico

    OpenAIRE

    López Soto, Alfonso

    1990-01-01

    INTRODUCCION: El lupus eritematoso sistémico (LES) es una enfermedad autoinmune que se caracteriza por la producción de numerosos autoanticuerpos. Recientemente, se ha descrito un nuevo grupo denominado anticuerpos antifosfolípido (AAF), cuyo estudio ha despertado un gran interés por sus implicaciones clínicas y patogenéticas. Los AAF son un grupo heterogéneo de autoanticuerpos adquiridos de forma espontánea, que van dirigidos contra estructuras fosfolipídicas de las membranas celulares.La de...

  4. Cubeta para la incubación y lavado de anticuerpos

    OpenAIRE

    Revilla Novella, Yolanda; Nogal París, María Luisa

    2008-01-01

    Cubeta para la incubación y lavado de anticuerpos. La cubeta comprende un cuerpo (1) lavable, inerte y no poroso, que está dotado de unos pocillos (2) ubicados en su interior, cuyo número y tamaño puede variar en función del requerimiento experimental. En dichos pocillos (2) se introducen preferiblemente fragmentos o tiras (3) de nitrocelulosa, que contienen proteínas que han sido previamente transferidas desde un gel de poliacrilamida utilizando un campo eléctrico. Esta cubeta ti...

  5. Detection of S-gene 'a' determinant variants in hepatitis B patients with both positive HBsAg and HBsAb markers

    International Nuclear Information System (INIS)

    Wu Yueping; Ling Yongwu; Huang Songping; Wang Shipeng; Chen Yufeng; Mao Liping; Lu Jianrong

    2005-01-01

    Objective: To explore the S-gene 'a' determinant variants in hepatitis B patients with both positive HBsAg and HBsAb markers and the effect on the antigenicity of HBsAg. Methods: Quantitative determination of HBV - DNA with competent PCR microfluidic chit method was performed in eight sera specimens from seven hepatitis B patients with both positive HBsAg and HBsAb markers. HBV S-gene was amplified with nested PCR, the PCR product was directly examined for any sequence variant of the amino acids. HBV markers were tested with the very sensitive ELISA/MEIA method in these seven patients. The above rests were also performed in 15 children after failed immunization with hepatitis B vaccine and 9 recipients of liver transplantation for terminal hepatitis B treated with HBIG and lamivudine, serving as controls. Results: The HBsAb contents in the seven patients were all below 80 mIu/ml. Two of the patients with positive HBV-DNA showed no 'a' determinant variant. Two of the four HBV-DNA negative patients demonstrated amino-acid variants (126, 131). One patients who was originally HBV-DNA positive but later turned negative after treatment with interferon and lamivudine demonstrated variant (126). In the 9 patients after successful liver transplantation, the HBsAb contents were all about 150mIu/ml with negative HBV-DNA and no variant. In the 15 immunization failures, HBV-DNA was positive in 14 of them, with 2 cases of variant at 145, 1 case at 126 and 1 case at 134. Conclusion: In some patients with chronic B hepatitis with both positive HBsAg and HBsAb markers, as well as in some vaccine immunization failures, there were 'a' determinant variants, which might alter the antigenicity of HBsAg with escape from the neutralization of low HBsAb. The 'a' determinant variant might also affect the replication of the virus. In this study, no variant was shown in patients after successful liver transplantation. However, the number of patients was too small and the result was of no

  6. [Comparison of the clinical performance of the ECLusys HBsAg II assay with the Lumipulse f and HISCL 2000-i HBsAg screening assays].

    Science.gov (United States)

    Sugiura, Aya; Iwahara, Kunihiro; Suga, Yasuyuki; Uchiyama, Sachinori; Maekawa, Masato

    2012-02-01

    We compared the ECLusys HBsAgII (ECL HBsAg) assay to the Lumipulse Forte (LPf HBsAg) and HISCL (HIS HBsAg) assays. Measurement of dilution panels for which the WHO HBsAg international reference panel was the parent specimen revealed that the ECL and HIS assays enabled detection to a theoretical level of 0.04 IU/mL, whereas the LPf assay enabled detection to a level of 0.08 IU/mL. In a specificity test using high RF positive specimens (n = 33), pregnancy specimens (n = 35), cytomegalovirus antibody positive specimens (n = 36), and high M protein positive specimens (n = 21) that were confirmed negative for HBsAg by the LPf assay, negative results were obtained for all specimens on the HIS assay, but the ECL assay yielded a positive result for one of the high RF positive specimens. This individual was suggested on further testing to be an HBV carrier who was strongly positive for HBc antibody. In HBsAg mutants detection test, the detection rate was 92.3% with the ECL assay and 69.2% with the HIS assay. In a correlation test using routinely collected clinical specimens (n = 155), including positive stock specimens, aside from the one case where the LPf assay gave a negative result but both the ECL and HIS assays gave positive results, all of the results were consistent for all specimens. The above results confirmed that the ECL assay is both highly sensitive and specific, and also enables a high rate of HBsAg mutant detection.

  7. Frecuencia de anticuerpos contra el virus C de la hepatitis en pacientes con cirrosis hepática en Yucatán, México

    Directory of Open Access Journals (Sweden)

    Góngora-Biachi Renán A

    2003-01-01

    Full Text Available OBJETIVO: En este estudio reportamos la prevalencia de anticuerpos contra el virus C de la hepatitis (Ac-VCH en un grupo de pacientes con cirrosis hepática (CH. MATERIAL Y MÉTODOS: Se hizo un estudio prospectivo, transversal y descriptivo, de marzo de 1998 a mayo de 1999. Se estudiaron a 153 pacientes (117 (76% hombres y 36 (24% mujeres con diagnóstico de CH, que eran atendidos en el Hospital General Agustín O' Horan y en el Centro de Investigaciones Regionales Doctor Hideyo Noguchi, en la ciudad de Mérida, Yucatán, México. Se aplicó un cuestionario con datos clínico-epidemiológicos y se determinó la presencia de Ac-VCH (ELISA de 2ª generación y RIBA-2 para confirmar el diagnóstico a cada paciente. Se determinó también el antígeno de superficie de la hepatitis B (AgsHB y anticuerpos contra el antígeno central de la hepatitis B (Anti-HBc mediante el método de ELISA. La presencia de Ac-VCH fue relacionada con las variables epidemiológicas de los sujetos. La prevalencia de anti-HCV y la frecuencia de características se compararon entre los pacientes positivos y negativos con las pruebas de c² y exacta de Fisher. RESULTADOS: El 32% de los pacientes con CH (35/117 (30% hombres y 14/36 (39% mujeres fueron positivos para los Ac-VCH. El alcoholismo estuvo presente en todos los hombres serorreactivos y en ninguna de las mujeres positivas (p< 0.001. Ninguna de las variables epidemiológicas analizadas estuvo asociada con la seropositividad. Los anti-HBc se encontraron en 16% de los pacientes positivos para Ac-VCH y en 12% de los seronegativos (p=0.69. CONCLUSIONES: La prevalencia encontrada fue mayor a los reportes previos realizados en población general de la Península de Yucatán (1.3%. La alta prevalencia de Ac-VCH en este grupo de pacientes sugiere que la CH está más frecuentemente asociada con el VCH en Yucatán, México, que con el virus B de la hepatitis. El alcoholismo probablemente actúa como un cofactor en el

  8. Faster heme loss from hemoglobin E than HbS, in acidic pH: Effect ...

    Indian Academy of Sciences (India)

    which are predominantly localized in the inner leaflet of erythrocytes. Absorption measurements indicated such loss .... Human blood samples, taken for diagnosis from patients with hemoglobin disorder, were analysed by ... patients with HbE ranging from 90% to 92% and HbS from homozygous HbS individuals with 90% to ...

  9. Delayed clearance of serum HBsAg in compensated cirrhosis B

    DEFF Research Database (Denmark)

    Fattovich, G; Giustina, G; Sanchez-Tapias, J

    1998-01-01

    The aim of this study was to evaluate the incidence, prognostic factors and clinical significance of delayed clearance of serum HBsAg in compensated cirrhosis B.......The aim of this study was to evaluate the incidence, prognostic factors and clinical significance of delayed clearance of serum HBsAg in compensated cirrhosis B....

  10. Estudo da soroprevalência do AgHBs em gestantes da 15ª Regional de Saúde e da imunoprofilaxia para os recém-nascidos das gestantes AgHBs positivo = Study into the HBsAg seroprevalence in pregnant women from the 15th Health Regional and the immunoprophylaxia on the newborns of these HBsAg-positive women

    Directory of Open Access Journals (Sweden)

    Sonia Kaori Miyamoto

    2008-01-01

    Full Text Available Determinar a prevalência do AgHBs nas gestantes da 15ª Regional de Saúde (15ª R.S. atendidas no Laboratório de Ensino e Pesquisa em Análises Clínicas (Lepac, Universidade Estadual de Maringá, e verificar se foi solicitada a imunoprofilaxia para hepatite B aos recém-natos das gestantes AgHBs positivo, no período de janeiro de 1998 a dezembro de 2002. A pesquisa do AgHBs foi realizada pela técnica imunoenzimática IMxHBsAg e Axsym HBsAg (Laboratório Abbott. As solicitações de imunobiológicos especiais para a imunoprofilaxia da hepatite B ao CRIE foram obtidas na Seção de Epidemiologia da 15ª R.S. Foram analisadas 12.274 gestantes e a prevalência do AgHBs foide 1,0%. Dentre as 125 gestantes AgHBs positivo, foram solicitadas imunoprofilaxia para 32 (25,6% recém-nascidos. Ainda que a prevalência encontrada indique ser esta uma área de baixa endemicidade, os resultados reafirmam a importância da realização do diagnóstico da hepatite B no atendimento pré-natal, para adoção da imunoprofilaxia no recém-nascido.To determine HBsAg prevalence among pregnant women from the 15th Health Regional assisted in the Laboratório de Ensino e Pesquisa em Análises Clínicas (Lepac, Universidade Estadual de Maringá, and verify whether immunoprophylaxis with vaccine and immunoglobulin was requested for the newborns of HBsAg-positive women during the period from January 1998 to December 2002. The research about HBsAg was conducted using the immunoenzymatic IMxHBsAg (Abbot Lab and Axsym HBsAg (Abbot Lab techniques. The requests to the CRIE for specialimmunobiologic agents for Hepatitis B immunoprophylaxis were obtained in the Department of Epidemiology from the 15th Health Regional. The analysis includes 12,274 pregnant women, and the HBsAg prevalence was 1.0%. Among 125 HBsAg positive pregnant women, immunoprophylaxis was requested for 32 (25.6% newborns. Although the prevalence detected demonstrates this to be a low endemic area, the

  11. Inmunodeficiencias primarias por déficit de anticuerpos en adultos

    Directory of Open Access Journals (Sweden)

    María Mercedes Bello Rodríguez

    2014-10-01

    Full Text Available Se realizó una investigación sobre los pacientes con inmunodeficiencias primarias por déficit de anticuerpos, atendidos en el servicio de inmunología del Hospital General “Dr. Ernesto Guevara” de Las Tunas, con el objetivo de caracterizar las inmunodeficiencias por déficit de anticuerpos en pacientes adultos, atendiendo al tipo de inmunoglobulina afectada, el sexo y la edad, así como las principales manifestaciones clínicas. Se evaluaron un total de diez pacientes tratados por esta causa entre los años 2005 y 2013. Para la evaluación de los resultados se utilizó el análisis de frecuencias simple. Las inmunodeficiencias diagnosticadas se correspondieron en un 50% con el déficit de las tres clases de inmunoglobulinas. Predominó el sexo masculino en un 70% y las edades más afectadas se correspondieron con la cuarta y quinta décadas de la vida. Las infecciones respiratorias y digestivas fueron las más frecuentes (80%, tanto por gérmenes bacterianos como virales, la infección por Giardia lamblia también apareció muy frecuentemente en los pacientes estudiados

  12. Obtención, purificación y caracterización de anticuerpos policlonales IgY desarrollados en gallina, dirigidos contra aislamientos colombianos de Giardia duodenalis.

    Directory of Open Access Journals (Sweden)

    Dabeiba Adriana García

    2005-12-01

    Full Text Available Introducción. El desarrollo de anticuerpos policlonales requiere de animales de laboratorio, generalmente, el conejo, que deben sangrarse para su obtención. Como alternativa, se han utilizado las gallinas. Objetivo. Desarrollar anticuerpos policlonales IgY anti-Giardia duodenalis y evaluar diferentes métodos para su purificación a partir de yema de huevo. Materiales y métodos. Se inmunizaron tres gallinas intramuscularmente con trofozoítos del parásito: las inmunizaciones se realizaron a los 0, 15, 30, 45, 60, 90 y 120 días. Se recolectaron huevos en cada etapa de la inmunización y se purificó la IgY por deslipidación (D y precipitación (P mediante cinco protocolos diferentes: M1: (P: sulfato de amonio/D: dextrán sulfato-cloruro de calcio, M2: (D: dextrán sulfato-cloruro de calcio/P: sulfato de amonio, M3: (D: cloroformo/ P: sulfato de amonio 50%, M4: (D: solución A/P:solución B y M5: (D: cloroformo/P: sulfato de amonio 30%. Se realizó evaluación inmunoquímica de la IgY anti-Giardia duodenalis mediante inmunodifusión, contrainmunoelectroforesis e inmunoelectrotransferencia (Western blot; la pureza de IgY por SDS-PAGE en presencia y ausencia de reductor y la concentración de la inmunoglobulina (mg/mL por espectrofotometría y densitometría. Resultados. La IgY anti-G. duodenalis mediante evaluación inmunoquímica presentó títulos hasta de 1:32. La inmunoglobulina en ausencia de reductor mostró una banda de 180 kd y en su presencia bandas de 30 y 68 kd, características de sus cadenas liviana y pesada, respectivamente. Las mayores concentraciones de inmunoglobulina se recuperaron con el método dos (M2, 4,6 mg de IgY por mL de yema de partida. Conclusiones. Por su facilidad y economía de producción en gallinas, los anticuerpos policlonales IgY anti-Giardia así obtenidos podrán utilizarse para desarrollar inmunoensayos que detecten el parásito en eluídos de heces.

  13. OBTENCIÓN DE ANTICUERPOS POLICLONALES IgY ANTIPARVOVIRUS CANINO A PARTIR DE YEMA DE HUEVO DE GALLINA

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    Pinto, J.

    2005-06-01

    Full Text Available Inmunizando un grupo de diez gallinas de postura raza Lohmann de 16 semanas de edad con parvovirus canino (CPV cepa vacunal, se obtuvieron anticuerpos IgY policlonales contra CPV en yema de huevo.En la extracción de los anticuerpos de la yema se requirieron dos pasos, el primero para la remoción de lípidos y el segundo para la precipitación de proteínas. Para la remoción de lípidos se usó el método PBS:Cloroformo y para la precipitación de los anticuerpos solubles (Inmunoglobulinas-IgY se usó el método de salting-out con sulfato de amonio ((NH42SO4. La evaluación del proceso se efectuó empleando el método comercial estándar “EGGstract IgY Purification System®” de PROMEGA.La metodología empleada permitió la obtención de anticuerpos IgY policlonales contra CPV a partir de yema de huevo de gallina en concentraciones altas por mililitro de yema, con una pureza aceptable y títulos altos; los resultados fueron comparables con el método comercial.

  14. ANTICUERPOS CONTRA EL VIRUS DE LA PARAINFLUENZA 3 EN CERDOS DE CRIANZA TECNIFICADA Y DE TRASPATIO BENEFICIADOS EN MATADEROS DE LIMA

    OpenAIRE

    Montesinos P., Milagros; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Rivera G., Hermelinda; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Araínga R., Mariluz; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

    2013-01-01

    El objetivo del presente estudio fue determinar la frecuencia de anticuerpos contra el virus de la Parainfluenza 3 (VPI3) en porcinos beneficiados en la ciudad de Lima. Se colectaron muestras de sangre de cerdos de ambos sexos y de 2 a 6 meses de edad, provenientes de granjas tecnificadas (n=192) y de crianza de traspatio (n= 192) para la detección de anticuerpos contra el VPI3 mediante la técnica de neutralización viral. El 5.5 ± 2.3% (21/384) de las muestras tuvieron anticuerpos contra el V...

  15. HBsAg positivity during pregnancy and adverse maternal outcomes: a retrospective cohort analysis.

    Science.gov (United States)

    Tan, J; Liu, X; Mao, X; Yu, J; Chen, M; Li, Y; Sun, X

    2016-10-01

    Hepatitis B virus infection characterized by HBsAg positivity during pregnancy is a well-recognized issue in developing countries, but the association between HBsAg positivity and adverse maternal outcomes remains uncertain. To examine the association between HBsAg positivity during pregnancy and adverse maternal outcomes, a retrospective cohort study was conducted in Sichuan province, China. Deliveries were recorded from six hospitals between 1 January 2009 and 31 December 2010. Pre-eclampsia, gestational diabetes mellitus (GDM), postpartum haemorrhage (PPH), intrahepatic cholestasis, Caesarean section and placenta previa were prespecified adverse maternal outcomes. We used two multivariate logistic regression models to assess the association between HBsAg positivity and adverse maternal outcomes. In total, 948 (4.2%) pregnant women were HBsAg positive from 22 374 deliveries. Pregnant women with positive HBsAg had higher risk of GDM (aOR1.41, 95%CI 1.15-1.74), PPH (1.44, 1.13-1.83), intrahepatic cholestasis (1.74, 1.40-2.16) and Caesarean section (1.24, 1.06-1.45). No statistical associations were found between HBsAg positivity and pre-eclampsia (1.36, 0.94-1.97), and placenta previa (1.21, 0.87-1.67). HBsAg positivity during pregnancy was associated with higher risk of multiple adverse maternal outcomes. Although the causality has yet to be established, efforts may be warranted in routine care, particularly in those with high risk for adverse maternal outcomes, given the volume population infected with HBsAg. Future studies are needed to establish causality and examine the impact of HBeAg on the adverse outcomes. © 2016 John Wiley & Sons Ltd.

  16. Hepatitis B Viral DNA Among HBs Antigen Negative Healthy Blood Donors

    OpenAIRE

    Vaezjalali, Maryam; Rashidpour, Shabnam; Rezaee, Hanieh; Hajibeigi, Bashir; Zeidi, Majid; Gachkar, Latif; Aghamohamad, Shadi; Najafi, Ronak; Goudarzi, Hossein

    2013-01-01

    Background Presence of occult hepatitis B infection (OBI) renders HBs antigen (HBsAg) undetectable by ELISA. Therefore it is valuable to evaluate the frequency of OBI among healthy blood donors to improve and perhaps change the strategies of blood screening to reduce the risk of HBV transmission. Objectives The aim of this study was to determine the presence of HBcAb and HBV DNA among Iranian HBsAg negative healthy blood donors who donated their blood to the Tehran Blood Transfusion Center du...

  17. Detección de anticuerpos antiplasmodium por ELISA en donantes de sangre

    Directory of Open Access Journals (Sweden)

    Patricia Olaya de Morales

    1982-06-01

    Full Text Available La malaria, una enfermedad transmitida por mosquitos del genero anopheles, puede ser inducida a través de transfusiones de sangre infectada con alguna de las especies de Plasmodium que afectan al hombre. Con el objeto de determinar el riesgo potencial de infección inducida por transfusiones, se analizaron durante 9 meses y mediante la técnica de E.L.I.S.A., las muestras de suero tomadas a los donantes de sangre del Hospital Militar Central de Bogotá. El 8.6 por mil de las 3114 muestras analizadas, resultaron positivas para anticuerpos antimaláricos y durante el tiempo del estudio fueron detectados 3 casos de malaria inducida por transfusiones.

  18. Comparative study of methods of detection of hepatitis ′B′ surface antigen (HBsAg.

    Directory of Open Access Journals (Sweden)

    Parab V

    1989-04-01

    Full Text Available The serum samples were collected from 52 patients of acute viral hepatitis and 235 hospital staff from Kasturba Hospital for Infectious Diseases. HBsAg was detected in their sera by counter-immuno-electrophoresis (CIEP, reverse passive hemogglutination (RPHA and by micro-enzyme-linked-immunosorbent assay (ELISA technique. Among the patients, HBsAg was detected in 12 cases (23% by CIEP, in 18 cases (34% by RPHA and in 23 patients (45% by ELISA. In the hospital staff, HBsAg was detected in 4 samples (1.7% by CIEP, in 8 samples (3.5% by RPHA and in 32 samples (13.5% by ELISA. Thus ELISA was found to be the most sensitive technique in detecting HBsAg.

  19. [Characteristics of HBV transmission in families with HBsAg-positive fathers and familial clustering of HBV infection].

    Science.gov (United States)

    Yang, Y; Jin, L; He, Y L; Liu, J F; Wang, J; Wang, K; Ma, X H; Li, Q; Feng, Y L; Yan, Z; Yi, R T; Chen, T Y; Zhao, Y R

    2016-04-01

    To investigate the characteristics of hepatitis B virus (HBV) transmission among family members in families with familial clustering of HBV infection and poor outcomes, as well as the prevalence and distribution characteristics of HBsAg in offspring with different parental HBsAg status. The general information of each member in families with poor outcomes were collected from 2007 to 2010, and serological test was performed to analyze the prevalence and distribution of HBsAg in family members. The chi-square test or Fisher's exact test was used to analyze and compare the sex of offspring and the prevalence of HBsAg in them in 266 nuclear families with different paternal and maternal HBsAg status. The positive rates of HBsAg in parents, siblings, children, and spouses of the probands were 20%, 88.2%, 76.8%, and 9.5%, respectively. The nuclear families with HBsAg-positive fathers and HBsAg-negative mothers had a significantly increased proportion of male offspring (male/female ratio = 2.02) compared with those with HBsAg-positive mothers and HBsAg-negative fathers (1.22) or those with HBsAg-negative fathers and mothers (0.96). In addition, in the nuclear families with HBsAg-positive fathers and HBsAg-negative mothers, the male offspring had a significantly higher HBsAg positive rate than female offspring (37.4% vs 13.8%), while in those with HBsAg-positive mothers and HBsAg-negative fathers or those with HBsAg-negative fathers and mothers, HBsAg positive rate showed no significant difference between male and female offspring. In families with familial clustering of HBV infection and poor outcomes, mother-to-child transmission is still the major route of HBV transmission, but father-to-child transmission also plays a role in HBV transmission in this special population. Positive HBsAg in fathers is associated with the increased proportion of male offspring, and father-to-son transmission of HBV is higher than father-to-daughter transmission.

  20. [Comparative Study for Anti-Hepatitis B Surface Antigen Titers Based on Two Measurement Methods: Using Monoclonal Antibodies Isolated from Hepatitis B Vaccinated Recipients].

    Science.gov (United States)

    Oone, Kumiko; Kani, Satomi; Oohashi, Minoru; Shinkai, Noboru; Inoue, Takako; Wakimoto, Yukio; Tanaka, Yasuhito

    2015-08-01

    As anti-hepatitis B surface antigen (anti-HBs) titers vary depending on the measurement methods, we compared two different methods to measure anti-HBs titers in sera and HBs monoclonal antibodies. The sera from 182 HB virus-resolved patients who were negative for HBsAg but positive for antiHB core protein (HBc) and/or anti-HBs were obtained. The measurement of anti-HBs was compared using either Lumipulse G1200 or Architect i2000SR. Six different monoclonal antibody (mAbs) clones isolated from healthy individuals inoculated with hepatitis B vaccine Bimmugen (genotype C) were used. A statistically significant correlation in anti-HBs titers was found between the two methods tested (Y = 0.951X + 100.7, R = 0.813, p Lumipulse and 12 (6.6%) were opposite results. Measuring 2 mAbs with HBV neutralizing activity, the titers of the 116 antibody (1.0 μg/mL) were comparable (689.3 mIU/mL by Lumipulse and 440.7 mIU/mL by Architect), whereas those of the 478 antibody (1.0 μg/mL) were much lower by Architect than by Lumipulse (42.6 vs. 818.6 mIU/mL, respectively). Of four other mAbs without HBV neutralizing activity, equal titers were observed for one; two mAbs had less anti-HB titers by Architect; and one was below the cut-off index (Lumipulse, and the potential ability to detect the 478 antibody with neutralizing activity is low, indicating that Architect might underestimate anti-HBs titers. Future studies should standardize the anti-HBs titer measurement system.

  1. Occult Hbv Infection in Hbsag Negative and Anti-Hbc Positive Blood Donors.

    Directory of Open Access Journals (Sweden)

    A Shebanizadeh

    2007-04-01

    Full Text Available Introduction: In recent years with introduction of better screening tests, the risk of infection with transfusion-transmitted viruses has been reduced remarkably, although obtaining a zero-risk blood supply still remains international blood transfusion services goal. The routine test for detection of HBV infected blood samples is examination of HBsAg with ELISA method but in occult HBV infection, HBsAg is not detectable by ELISA. Therefore, a more sensitive or complementary test is needed. Some international blood transfusion services have introduced anti-HBc screening as a surrogate test for the presence of HBV infection. The aim of this study was to evaluate the prevalence of occult HBV infection in Isfahanian blood donors and the potential value of anti-HBc testing of donors as a screening test to detect occult HBV infection. Methods: In this descriptive cross-sectional study, 545 blood units were collected (from Isfahan blood center and tested by HBsAg ELISA kit from April to June 2004 and then all HBsAg negative samples were tested by anti-HBc ELISA kit. To detect occult HBV infection, all HBsAg negative and anti-HBc positive samples were tested by PCR method. Results: All samples were negative for HBsAg while 43 blood units were anti-HBc positive (8%. These HBsAg negative and anti-HBc positive blood units were tested for HBV DNA of which five units (%11.6 were HBV DNA positive. Conclusion: Occult HBV infection is a clinical form of HBV infection that cannot be detected by usual method (ELISA for HBsAg and therefore more sensitive techniques are needed for detection of HBV infection. PCR is a sensitive technique that detects HBV DNA even in a trace mounts. Our results identified that more sensitive and complementary tests such as, PCR and anti-HBc, are essential and helpful to ensure safety of blood units.

  2. Hepatitis B virus surface antigen and anti-hepatitis C virus rapid tests underestimate hepatitis prevalence among HIV-infected patients

    DEFF Research Database (Denmark)

    Hønge, Bo Langhoff; Jespersen, S; Medina, C

    2014-01-01

    OBJECTIVES: In the case of coinfection with HIV and hepatitis B virus (HBV) and/or hepatitis C virus (HCV), hepatic disease progression is often accelerated, with higher rates of liver cirrhosis and liver-related mortality. We aimed to evaluate the performance of the rapid tests used routinely...... to detect HBV surface antigen (HBsAg) and anti-HCV among HIV-infected patients in Guinea-Bissau. METHODS: Blood samples from HIV-infected patients in Guinea-Bissau were stored after testing for HBsAg and anti-HCV with rapid tests. Samples were subsequently re-tested for HBsAg and anti-HCV in Denmark...

  3. Triple positivity of HBsAg, anti-HCV antibody, and HIV and their ...

    African Journals Online (AJOL)

    tested positive to HIV/HBV/HCV triple infection before and after the commencement of HAART. Of these infections, ... Testing of these triple infections should be a big concern in the best choice and commencement of ART. Also, the study showed that ..... Godsworth, N.J., Cooper, D.A. and Disnovan, B. (2009). The influence ...

  4. Evaluation of the highly sensitive chemiluminescent enzyme immunoassay "Lumipulse HBsAg-HQ" for hepatitis B virus screening.

    Science.gov (United States)

    Deguchi, Matsuo; Kagita, Masanori; Yoshioka, Nori; Tsukamoto, Hiroko; Takao, Miyuki; Tahara, Kazuko; Maeda, Ikuhiro; Hidaka, Yoh; Yamauchi, Satoshi; Kaneko, Atsushi; Miyakoshi, Hideo; Isomura, Mitsuo

    2017-10-06

    Ongoing efforts in the development of HBsAg detection kits are focused on improving sensitivity and specificity. The purpose of this study was to evaluate an improved, highly sensitive quantitative assay, "Lumipulse HBsAg-HQ", a chemiluminescent enzyme immunoassay designed for a fully automated instrument, the "Lumipulse G1200". Serum samples for reproducibility, dilution, correlation, sensitivity, and specificity studies were obtained from patients at the Osaka University Hospital. Seroconversion and sensitivity panels were purchased from a commercial vender. Subtype, sensitivity panels, and HBsAg recombinant proteins with one or two amino acid substitutions were prepared in-house. The coefficients of variation for the low, medium, and high concentration samples ranged from 1.93 to 2.55%. The HBsAg-HQ reagent for dilution testing showed good linearity in the 0.005-150 HBsAg IU/mL range and no prozone phenomenon. All 102 HBV carrier samples were positive by HBsAg-HQ, while other commercial reagents showed one or more to be negative. In the seroconversion panel, the 14-day blood sample was positive. The sensitivity against HBsAg-HQ "ad" and "ay" subtypes was 0.025 ng/mL. Comparisons among the HBsAg-HQ, HISCL, and Architect HBsAg reagents were performed using the Bland-Altman plot. Specificity for 1000 seronegative individuals was 99.7%. HBsAg-HQ detected 29 positive serum among 12 231 routinely obtained serum samples, which showed concentrations of 0.005-0.05 HBsAg IU/mL. According to these results, the Lumipulse HBsAg-HQ assay, with a highly sensitive limit of detection of 0.005 IU/mL, may facilitate the development of a better management strategy for a considerable proportion of infected patients. © 2017 Wiley Periodicals, Inc.

  5. In Situ Liver Expression of HBsAg/CD3-Bispecific Antibodies for HBV Immunotherapy

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    Robert L. Kruse

    2017-12-01

    Full Text Available Current therapies against hepatitis B virus (HBV do not reliably cure chronic infection, necessitating new therapeutic approaches. The T cell response can clear HBV during acute infection, and the adoptive transfer of antiviral T cells during bone marrow transplantation can cure patients of chronic HBV infection. To redirect T cells to HBV-infected hepatocytes, we delivered plasmids encoding bispecific antibodies directed against the viral surface antigen (HBsAg and CD3, expressed on almost all T cells, directly into the liver using hydrodynamic tail vein injection. We found a significant reduction in HBV-driven reporter gene expression (184-fold in a mouse model of acute infection, which was 30-fold lower than an antibody only recognizing HBsAg. While bispecific antibodies triggered, in part, antigen-independent T cell activation, antibody production within hepatocytes was non-cytotoxic. We next tested the bispecific antibodies in a different HBV mouse model, which closely mimics the transcriptional template for HBV, covalently closed circular DNA (cccDNA. We found that the antiviral effect was noncytopathic, mediating a 495-fold reduction in HBsAg levels at day 4. At day 33, bispecific antibody-treated mice exhibited 35-fold higher host HBsAg immunoglobulin G (IgG antibody production versus untreated groups. Thus, gene therapy with HBsAg/CD3-bispecific antibodies represents a promising therapeutic strategy for patients with HBV.

  6. A vaccine prepared from the 22 nm particles of surface hepatitis B antigen (HBsAg)

    International Nuclear Information System (INIS)

    Karelin, V.P.; Babaeva, E.E.; Gubenko, E.F.; Kaulen, D.K.; Zhdanov, V.M.

    1980-01-01

    A method for obtaining a subunit inactivated vaccine preparation from the 22-nm particles of HBsAg is proposed. For inactivation of the residual infectious hepatitis B virus (HBV) the preparations were successively treated with 1% sodium dodecyl sulfate (SDS) and nucleases. In addition, thermal denaturation and ultraviolet irradiation of HBV DNA were used. As a control the biologic activity of a reference virus (SV40) was tested after the same treatment. The effectiveness of DNA inactivation was monitored by adding 3H-thymidine labeled reference virus to the vaccine preparations. The purified and inactivated HBsAg was adsorbed on Al2O3. Antigenicity was calculated on the basis of the determination of antibody in guinea pigs immunized with various doses of the vaccine, and the release of 125 I- HBsAg from blood and kidneys in immunized and control mice was analyzed. Possible methods of inactivation and control of HBV vaccine is discussed

  7. Association of social class in HBsAg and hepatocellular carcinoma

    International Nuclear Information System (INIS)

    Pervez, T.; Anwar, M. S.

    2001-01-01

    Objective: To find out the social class difference in relation to frequency of HBsAg and hepatocellular carcinoma in our population. Design: An analytical study. Place and Duration of Study: This study was conducted in Oncology Department, Services Hospital, Lahore from December 1997 to December 2000. Subjects and Methods: The HBsAg positive voluntary and apparently healthy blood donors were grouped into three, based on monthly income. Lower socioeconomic group and had monthly income less than 3,000 Pakistani rupees, middle socioeconomic group had monthly income between 3,000-10,000 rupees and upper socioeconomic group had income of more than 10,000 Pakistani rupees. On the same pattern patients suffering from hepatocellular carcinoma coming for treatment were also grouped. During this period, 1000 blood donors were screened for HBsAg and 95 biopsy proven liver cancer by causes were treated. Medical and demographic data of all subjects were recorded. HBsAg test was performed immuno-chromatographic technique using Daina Screen HBsAg kit manufactured by Dainabot Co. Ltd, Tokyo, Japan. Results: Patients from lower and middle social class had higher percentage (80% and 75%) of hepatocellular carcinoma as compared to higher social class (66.6%). In the healthy asymptomatic blood donors lower social class had higher (13.76%) HBsAg positively as compared to middle social class (11.25%) and higher social class (8.06%). Conclusion: Preventive measures should be taken in identifying and reducing factors predisposing high frequency of these conditions. (author)

  8. Hepatitis B viral DNA among HBs antigen negative healthy blood donors.

    Science.gov (United States)

    Vaezjalali, Maryam; Rashidpour, Shabnam; Rezaee, Hanieh; Hajibeigi, Bashir; Zeidi, Majid; Gachkar, Latif; Aghamohamad, Shadi; Najafi, Ronak; Goudarzi, Hossein

    2013-03-01

    Presence of occult hepatitis B infection (OBI) renders HBs antigen (HBsAg) undetectable by ELISA. Therefore it is valuable to evaluate the frequency of OBI among healthy blood donors to improve and perhaps change the strategies of blood screening to reduce the risk of HBV transmission. The aim of this study was to determine the presence of HBcAb and HBV DNA among Iranian HBsAg negative healthy blood donors who donated their blood to the Tehran Blood Transfusion Center during 2011. 1000 serum specimens negative for HBsAg, HCV antibody and HIV antibody were collected from healthy blood donors and tested for HBcAb. Presence of hepatitis B viral DNA was checked in HBcAb positive samples by nested PCR with two sets of primers to amplify part of HBV S gene. There were 64 women and 936 men in the population under study. The mean ± SD age of the donors was 38 ± 11 years. 80 out of 1000 samples (8%) were found to be positive for HBcAb. HBV DNA was detected in 50% of HBcAb positive specimens. The mean ± SD age of donors without HBV DNA was 37.7 ± 10.5 years and for donors with HBV DNA was 40.9 ± 11.2 years (P = 0.05). OBI was prevalent among 50% of HBcAb positive healthy blood donors. The frequency of positive HBcAb among healthy HBsAg negative blood donors was comparable to previous studies reported from Iran. On the other hand, the frequency of HBV DNA in HBsAg negative blood donors was higher than previous reports.

  9. Bounding the flavor-violating Hbs vertex from the B → Xsγ decay

    International Nuclear Information System (INIS)

    Aranda, J I; Ramirez-Zavaleta, F; Tututi, E S; Montano, J; Toscano, J J

    2011-01-01

    The nondiagonal Hbs coupling within the context of an effective Yukawa sector that comprises SU L (2) x U Y (1)-invariant operators of up to dimension six is studied. The recent experimental result on B → X sγ with hard photons is employed to constrain the Hbs vertex, with which the branching ratio for the B s → γγ decay is estimated. It is found that the B s → γγ decay can reach a branching ratio of the order of 4 x 10 -8 .

  10. Reactivation of hepatitis B virus infection with persistently negative HBsAg on three HBsAg assays in a lymphoma patient undergoing chemotherapy.

    Science.gov (United States)

    Cheung, Wing-I; Chan, Henry Lik-Yuen; Leung, Vincent King-Sun; Tse, Chi-Hang; Fung, Kitty; Lin, Shek-Ying; Wong, Ann; Wong, Vincent Wai-Sun; Chau, Tai-Nin

    2010-02-01

    In patients with occult hepatitis B virus (HBV) infection, acute exacerbation may occur when they become immunocompromised. Usually, these patients develop hepatitis B surface antigen (HBsAg) seroreversion during the flare. Here we report on a patient with occult HBV infection, who developed HBV exacerbation after chemotherapy for diffuse large B-cell lymphoma. The resurgence of HBV DNA preceded the elevation of liver enzymes for 20 weeks. Atypically, despite high viraemia, serological tests showed persistently negative HBsAg using three different sensitive HBsAg assays (i.e., Architect, Murex and AxSYM). On comparing the amino acid sequence of the index patient with the consensus sequence, five mutations were found at pre-S1, five at pre-S2 and twenty-three mutations at the S region. Six amino acid mutations were located in the 'a' determinant, including P120T, K122R, M133T, F134L, D144A and G145A. The mutants K122R, F134L and G145A in our patient have not been tested for their sensitivity to Architect and Murex assays by the previous investigators and might represent the escape mutants to these assays.

  11. Acute hepatitis B virus infection with simultaneous high HBsAg and high anti-HBs signals in a previously HBV vaccinated HIV-1 positive patient

    NARCIS (Netherlands)

    van Dommelen, Laura; Verbon, Annelies; van Doorn, H. Rogier; Goossens, Valère J.

    2010-01-01

    We present a case of a clinical manifest hepatitis B virus infection and a potentially misleading HBV serological profile in an HIV-1 positive patient despite previous HBV vaccination. The patient presented with an acute hepatitis B and there was no indication of chronic HBV infection or the

  12. Uso de una conantokina y anticuerpos policlonales para identificar la subunidad NR2B del receptor

    Directory of Open Access Journals (Sweden)

    Leonardo Lareo,†

    2010-12-01

    Full Text Available Objetivo. Proponer una metodología de identificación de la subunidad NR2B, mediante el uso de conantokina G, así como una adecuada extracción de la subunidad NR2B. Materiales y métodos. Se ensayaron dos metodologías para la extracción de la subunidad NR2B de cerebro de rata adulta, la primera buscó la extracción de la subunidad a partir de la membrana mediante la utilización del detergente deoxicolato de sodio y la segunda, garantizó primero la solubilización y eliminación de proteínas citoplasmáticas para luego realizar la extracción de la subunidad mediante el uso del mismo detergente, a partir del pellet generado en la centrifugación del extracto obtenido. Adicionalmente se biotiniló la conantokina G para evaluar su eficiencia en la identificación de la subunidad y comparar los resultados con los obtenidos por metodologías tradicionales como DOT-BLOT, WESTERN-BLOT, ELISA e Inmunohistoquímica. Resultados. La segunda metodología mostró mayor extracción de NR2B por lo que se seleccionó para la realización de los extractos posteriores. Los ensayos de identificación con la conantokina biotinilada evidenciaron interferencia en el reconocimiento, haciéndose necesaria la identificación de la presencia de la subunidad NR2B mediante el uso de anticuerpos policlonales en los ensayos mencionados. Conclusiones. Se propone que hay un impedimento de tipo estérico en el marcaje de la conantokina con la biotina lo que no favorece la interacción de este péptido con la subunidad.

  13. Evaluación de la respuesta de anticuerpos hacia antígenos de Pseudomonas aeruginosa.

    Directory of Open Access Journals (Sweden)

    Aniel Moya

    2007-04-01

    Full Text Available Pseudomonas aeruginosa es un patógeno extracelular que genera una respuesta de anticuerpos específicos con utilidad para el diagnóstico y vacunas. En el presente estudio nos propusimos evaluar en suero humano los niveles de anticuerpos contra antígenos relevantes de P. aeruginosa. Realizamos la determinación de anticuerpos IgG contra tres exoenzimas, consideradas como factores de virulencia de mayor importancia en infecciones. Este resultado dio paso a la evaluación del reconocimiento de IgG e IgA hacia antígenos de la envoltura celular bacteriana por ELISA de células enteras. Todos los sueros evaluados mostraron títulos de IgG e IgA superiores a los individuos sanos, con excepción de dos muestras de pacientes que no mostraron alto título. Este ensayo permitió analizar el nivel de reconocimiento hacia los antígenos más expuestos de la bacteria que incluyen principalmente LPS y proteínas de membrana externa. Se encontró diferencias entre los valores de densidad óptica a 450 nm de individuos sanos y enfermos. El método usado permitió seleccionar dos sueros de pacientes de diferentes tipos de infecciones que fueron comparados por Western blot. Se observó que aunque los sueros tenían reacción hacia distintos serotipos de P. aeruginosa, la intensidad del reconocimiento variaba según el tipo de infección.

  14. Encefalitis por anticuerpos contra el receptor de NMDA: experiencia con seis pacientes pediátricos. Potencial eficacia del metotrexato

    Science.gov (United States)

    Bravo-Oro, Antonio; Abud-Mendoza, Carlos; Quezada-Corona, Arturo; Dalmau, Josep; Campos-Guevara, Verónica

    2016-01-01

    Introducción La encefalitis por anticuerpos contra el receptor de N-metil-D-aspartato (NMDA) es una entidad cada vez más diagnosticada en edad pediátrica. A diferencia de los adultos, en muchos casos no se asocia a tumores y las manifestaciones iniciales en niños más frecuentes son crisis convulsivas y trastornos del movimiento, mientras que en los adultos predominan las alteraciones psiquiátricas. Casos clínicos Presentamos seis casos pediátricos confirmados con anticuerpos contra la subunidad NR1 del receptor de NMDA en suero y líquido cefalorraquídeo. Cinco de los casos comenzaron con crisis convulsivas como manifestación clínica inicial antes de desarrollar el cuadro clásico de esta entidad. En todos los casos se utilizaron esteroides como primera línea de tratamiento, con los que sólo se observó control de las manifestaciones en uno, por lo que el resto de los pacientes requirió inmunomoduladores de segunda línea. Todos los pacientes recibieron metotrexato como tratamiento inmunomodulador para evitar recaídas y la evolución fue a la mejoría en todos ellos. Conclusiones En nuestra serie de pacientes con encefalitis por anticuerpos contra el receptor de NMDA, ninguno se asoció a tumores. Todos los casos recibieron metotrexato por lo menos durante un año, no observamos eventos adversos clínicos ni por laboratorio, ni hubo secuelas neurológicas ni recaídas durante el tratamiento. Aunque es una serie pequeña y es deseable incrementar el número y tiempo de evolución, consideramos el metotrexato una excelente alternativa como tratamiento inmunomodulador para esta patología. PMID:24150952

  15. POLYCLONAL ANTIBODIES TO DETECT THE CP-RT PROTEIN OF Potato Mop-Top VIRUS ANTICUERPOS POLICLONALES PARA LA DETECCIÓN DE LA PROTEÍNA CP-RT DEL Potato Mop-Top VIRUS

    Directory of Open Access Journals (Sweden)

    Derly Andrade Molina

    2012-06-01

    Full Text Available Abstract. Potato mop-top virus (PMTV causes an important re-emerging disease in potato crops in Colombia due to the increased incidence of its protozoan vector Spongospora subterranea f.sp. subterranea (Sss, the causing agent of Powdery scab disease. For an accurate detection of PMTV it is recommended to combine different diagnostic tests and evaluate multiple samples per plant or tissue. In order to increase the number of available tools for detection of PMTV, antibodies targeting the RT domain of the CP-RT protein were developed in this work. Sequencing of the RT domain from the colombian strain R25 was achieved and using bioinformatic analysis, a potential antigenic region was identified. A peptide mimicking the antigenic region was inoculated in rabbits for the production of polyclonal antibodies. The antibodies were tested by ELISA using Nicotiana benthamiana bait-plants infected with Sss cystosori and potato plants collected in La Unión (Antioquia. The validity of serological tests was confirmed by RT-PCR. A complete sequence of the RT domain and 441 nt of the CP gene were obtained. Phylogenetic analysis identified strain R25 as closely associated to the PMTV lineage distributed worldwide. A total of 19.26 mg of anti-CP-RT polyclonal antibodies useful in detecting PMTV in infected plants were obtained. As CP-RT is involved in transmission of PMTV by Sss, these antibodies will be useful for supporting not only diagnostic programs but also basic and epidemiologic studies aimed at understanding interactions between PMTV and Sss.Resumen. El Potato mop-top virus (PMTV es uno de los virus re-emergentes en los cultivos de papa de Colombia, como resultado del aumento de la incidencia de su vector natural, el protozoo Spongospora subterranea f.sp. mamarinm@unal.edu.co (Sss, agente causal de la Sarna polvosa de la papa. Para la detección del virus se recomienda la realización simultánea de diferentes pruebas, así como la evaluación de m

  16. Construction and immunological evaluation of truncated hepatitis B core particles carrying HBsAg amino acids 119–152 in the major immunodominant region (MIR)

    Energy Technology Data Exchange (ETDEWEB)

    Su, Qiudong; Yi, Yao [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China); Guo, Minzhuo [Beijing Entry-Exit Inspection and Quarantine Beureau, Tianshuiyuan Lane 6, Chaoyang District, Beijing 100026 (China); Qiu, Feng; Jia, Zhiyuan; Lu, Xuexin; Meng, Qingling [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China); Bi, Shengli, E-mail: shengli_bi@163.com [National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206 (China)

    2013-09-13

    Highlights: •The conformational HBV neutralization antigen domain was successfully displayed on the surface of truncated HBc particles. •Appropriate dialysis procedures to support the renaturing environment for the protein refolding. •Efficient purification procedures to obtain high purity and icosahedral particles of mosaic HBV antigen. •Strong immune responses not only including neutralization antibody response but also Th1 cell response were induced in mice. -- Abstract: Hepatitis B capsid protein expressed in Escherichia coli can reassemble into icosahedral particles, which could strongly enhance the immunogenicity of foreign epitopes, especially those inserted into its major immunodominant region. Herein, we inserted the entire ‘α’ antigenic determinant amino acids (aa) 119–152 of HBsAg into the truncated HBc (aa 1–144), between Asp{sup 78} and Pro{sup 79}. Prokaryotic expression showed that the mosaic HBc was mainly in the form of inclusion bodies. After denaturation with urea, it was dialyzed progressively for protein renaturation. We observed that before and after renaturation, mosaic HBc was antigenic as determined by HBsAg ELISA and a lot of viruslike particles were observed after renaturation. Thus, we further purified the mosaic viruslike particles by (NH{sub 4}){sub 2}SO{sub 4} precipitation, DEAE chromatography, and Sepharose 4FF chromatography. Negative staining electron microscopy demonstrated the morphology of the viruslike particles. Immunization of Balb/c mice with mosaic particles induced the production of anti-HBs antibody and Th1 cell immune response supported by ELISPOT and CD4/CD8 proportions assay. In conclusion, we constructed mosaic hepatitis core particles displaying the entire ‘α’ antigenic determinant on the surface and laid a foundation for researching therapeutic hepatits B vaccines.

  17. Quantification of HBsAg to predict low levels and seroclearance in HBeAg-negative patients receiving nucleos(tide analogues.

    Directory of Open Access Journals (Sweden)

    Teresa Broquetas

    Full Text Available HBeAg-negative chronic hepatitis B patients require long-term nucleos(tide analogues(NAs because loss of surface antigen (HBsAg is unusual. Low quantitative HBsAg (qHBsAg levels can identify patients with higher probability of seroclearance. The aim of our study was to evaluate qHBsAg in HBeAg-negative patients receiving NAs to predict a reduction of HBsAg levels and seroclearance.Retrospective analysis of qHBsAg in HBeAg-negative patients before and at years 1, 3, 5, 8 and over of NAs treatment.From 1999 to 2015, HBsAg was quantified in 358 serum samples from 95 HBeAg-negative patients. Low qHBsAg (0.3 log IU/mL showing a positive and negative predictive value of 42% and 100% to identify patients achieving low levels of HBsAg.Reduction of qHBsAg is slow in HBeAg-negative patients receiving NAs, although low levels or faster qHBsAg decline may occur in 14%. A qHBsAg reduction >0.3 log IU/mL at year 3 can identify patients with a higher probability of achieving low levels and HBsAg seroclearance.

  18. Estudio del perfil de anticuerpos contra Helicobacter pylori producidos por pacientes con diferentes patologías gastroduodenales

    Directory of Open Access Journals (Sweden)

    Olga María Bermúdez

    2004-07-01

    de la subclase 1 en los pacientes con gastritis atrófica y adenocarcinoma gástrico, indica que la presencia de anticuerpos hacia esta proteína podría ser usada como un marcador de cáncer, mientras que el estudio del reconocimiento de los demás antígenos principales (70 kDa, 90 kDA y VacA puede convertirse en una herramienta de importante valor para la caracterización de cada enfermedad.

  19. Descripción de los anticuerpos, y manifestaciones clínicas de pacientes con polimiositis y dermatomiositis

    OpenAIRE

    Vega Castro, Kelly Patricia

    2014-01-01

    Introducción: La polimiositis y dermatomiositis, son enfermedades del grupo de miopatías inflamatorias, su curso es crónico, etiología autoinmune, tienen debilidad muscular simétrica de inicio proximal y la dermatomiositis se asocia a manifestaciones cutáneas. Genera importante situación de discapacidad física y emocional, costo social y económico elevado. La asociación de los anticuerpos circulantes con las manifestaciones clínicas, posibilidad de complicaciones y pronóstico ha tenido en los...

  20. Expression of hepatitis B surface antigen (HBsAg) gene in ...

    African Journals Online (AJOL)

    The transformed nature of the plants was confirmed by polymerase chain reaction (PCR) analysis and Southern blot hybridization. Expression of HBsAg was confirmed by western blotting and levels of expression were assayed by enzyme-linked immunosorbent assay (ELISA). Southern blot hybridization confirmed the ...

  1. Prevalence of Hepatitis-B Surface Antigen (HbsAg), Hepatitis C ...

    African Journals Online (AJOL)

    The prevalence of Hepatitis-B surface antigen (HBsAg), Hepatitis C virus (HCV) and Human immunodeficiency virus (HIV) was determined among apparently healthy male blood donors in Aminu Kano Teaching Hospital, Kano, between January and December, 2002. A total of 2,288 blood samples from the blood donors ...

  2. Hepatitis B Antigenaemia (HbS Ag): Risk Of Occupational Exposure ...

    African Journals Online (AJOL)

    The prevalence of Hepatitis B. surface Antigen (Hbs Ag) is high in sub-Saharan Africa, Nigeria inclusive. We set out to assess the risk of occupational exposure to Hepatitis B virus (HIV) infection in medical laboratory workers in Nigeria by screening 200 consecutive serum samples processed over a two week period at the ...

  3. Sero-prevalence of Hepatitis B Surface Antigen (HBsAg), in Sexually ...

    African Journals Online (AJOL)

    Sero-prevalence of Hepatitis B Surface Antigen (HBsAg), in Sexually Transmitted Disease Patients. ... partners in the past 6 months, a history of a number of episodes of STDs, history of heterosexual exposure to partners at risk, for example prostitutes; a history of symptoms of an STD at the commencement of the study.

  4. Utilidad de las muestras de sangre total desecada en papel de filtro en la detección de anticuerpos antitoxoplasma

    Directory of Open Access Journals (Sweden)

    Joaquim Gascon

    1989-04-01

    Full Text Available Se compara la técnica de Aglutinación Directa (AD utilizando muestras de sangre total desecada en papel de filtro, con la técnica de ELISA y la misma AD utilizando muestras de suero de los mismos pacientes, para la detección de anticuerpos antitoxoplasma. Los resultados muestran la validez del método de la sangre desecada en papel de filtro para la detección de anticuerpos antitoxoplasma con la técnica de AD, y se considera su utilidad en los estudios epidemiológicos de campo.

  5. The risk of transmission of hepatitis B from HBsAg(-), HBcAb(+), HBIgM(-) organ donors.

    Science.gov (United States)

    Wachs, M E; Amend, W J; Ascher, N L; Bretan, P N; Emond, J; Lake, J R; Melzer, J S; Roberts, J P; Tomlanovich, S J; Vincenti, F

    1995-01-27

    Liver allografts from HBcAb(+), IgM(-), HBsAg(-) donors can transmit HBV to uninfected recipients. We currently no longer accept these livers for transplantation while continuing to accept the kidneys. The purpose of this study is to determine the risk of donor-transmitted HBV infections from HBcAb(+), HBIgM(-), HBsAg(-) organ donors and determine if the risk of donor-transmitted HBV infections and their severity is dependent on the organ being transplanted. This study consists of a retrospective review of the posttransplant course of recipients of HBcAb(+), HBIgM(-), HBsAg(-) donors accepted at UCSF from 6/85 to 12/93. Transmitted HBV infection was defined as one in which the recipient changed from HBsAg(-) prior to transplantation to HBsAg(+) posttransplant, with no other source. There were 25 of 1190 donors who were HBcAb(+), HBIgM(-), HBsAg(-); 1/42 kidney, 3/6 liver, and 0/7 heart HBsAg(-) transplant recipients of organs from these donors became HBsAg(+) after transplantation. This difference in infection rate (liver vs. kidney and heart) is statistically significant. The clinical course of the liver recipients was also more severe. All of the patients who became infected were HBsAb(-) and HBcAb(-) prior to transplant. We conclude that (1) HBV can be transmitted from HBcAb(+), HBIgM(-), HBsAg(-) organ donors, (2) the rate of transmission is highest and severity of infection is worst in the liver recipients; and (3) we will continue to transplant kidneys from these donors, preferably into immunized recipients.

  6. Anti-dsdna, anti-smith y anti-la: Factores de riesgo y pronóstico de nefritis lúpica

    OpenAIRE

    Roberto Ferro; Francisco Medina; Demetrio Serracín

    2006-01-01

    Introducción:La nefritis lúpica es una manifestación común y de mal pronóstico en pacientes con lupus eritematoso sistémico. Diferentes; autoanticuerpos han mostrado relación con la aparición nefritis lúpica pero los resultados son controversiales. En la presente investigación; se estudian los factores inmunológicos asociados a la aparición y grado morfológico de nefritis lúpica, tales como el anticuerpo anti-dsDNA,; anti-Sm, anti-Ro y anti-La y anti-RNP. Materiales y Métodos:Estudio...

  7. Anti-hepatits B core antigen testing, viral markers, and occult hepatitis B virus infection in Pakistani blood donors: implications for transfusion practice.

    Science.gov (United States)

    Bhatti, Farhat Abbas; Ullah, Zia; Salamat, Nuzhat; Ayub, Muhammad; Ghani, Ejaz

    2007-01-01

    The purpose of this study was to determine the seroprevalence of anti-hepatitis B core antigen (HBc) and the impact of its testing along with other markers of hepatitis B, hepatitis B virus (HBV) DNA, hepatitis C virus antibody (anti-HCV), and syphilis in Pakistani blood donors. The study design was cross-sectional. A total of 966 donors were selected randomly for testing of anti-HBc and HBV markers, including HBV DNA, of 94,177 blood donors who were routinely screened for hepatitis B surface antigen (HBsAg), anti-HCV, human immunodeficiency virus antibody (anti-HIV), Treponema pallidum hemagglutination assay (TPHA), and malarial parasites from 2003 to October 2005. The seroprevalence of various infectious markers was as follows: HBsAg, 2.16 percent; anti-HCV, 4.16 percent; anti-HIV, 0.004 percent; TPHA, 0.75 percent; and malaria, 0.002 percent. Anti-HBc prevalence in HBsAg-negative, HBV DNA-negative blood donors was 167 of 966 (17.28%), with 76 percent demonstrating anti-HBs positivity. Younger donors with mean age of 25 years were exposed to HBV to a lesser extent compared to those with a mean age of 29 years. Anti-HBc positivity was significantly higher in anti-HCV-reactive individuals. HBV DNA was detectable in 5 blood donors who were HBsAg-, anti-HBc-positive and were categorized as having occult HBV infection. The study shows that more than 17 percent of healthy, young blood donors in Pakistan are already exposed to HBV, with two-thirds showing anti-HBs levels of greater than 100 mIU per mL. One in 200 blood donors who are HBsAg-, anti-HBc-positive, however, have occult HBV infection, with likelihood of transmission of hepatitis B in recipients of blood components derived from them. HBsAg-negative individuals who are anti-HBc-negative and those who are anti-HBc-positive, anti-HBs-positive, and HBV DNA-negative should be selected as regular blood donors to minimize transmission due to occult hepatitis B infection.

  8. Anticuerpos monoclonales contra la Gonadotropina Corionica Humana (HCG y su empleo en el diagnóstico precoz del embarazo.

    Directory of Open Access Journals (Sweden)

    Torres E

    2006-03-01

    Full Text Available Se describe la obtención y caracterización de anticuerpos monoclonales contra la hormona gonadotropina cariónica humana (HCG, seleccionados de la inmunización subcutánea en ratones BALB/c. Se reporta también el desarrollo del sistema rápido HeberFast Line® Embarazo, para la detección de la hormona en muestras de orina, basado en los anticuerpos obtenidos y la tecnología del flujo lateral desarrollada en membranas de nitrocelulosa como soporte sólido. El nivel de sensibilidad alcanzado con este sistema diagnóstico es de 20 UI/L de HCG en muestra de orina, y no se observó reactividad cruzada en analitos similares conteniendo concentraciones de las glicohormonas relacionadas; 1000 IU de LH, 5000 IU de FSH, y 1000 µIU de TSH respectivamente. Los estudios de estabilidad arrojaron, que el diagnosticador mantine las características de funcionalidad por un tiempo de 15 meses a temperaturas entre 2 y 30 ºC, conformando este el tiempo de vigencia del sistema. De poseer en Farmacias, Consultorios Médicos de la Familia y Hospital Gineco-Obstétricos, un sistema rápido y sencillo como el establecido en este trabajo, se pueden evitar todos los riesgos e inconvenientes que acarrean los procesos de interrupción del embarazo en la mujer.

  9. Prevalencia de anticuerpos contra Brucella sp. En donantes del banco de sangre de un hospital de Lima

    Directory of Open Access Journals (Sweden)

    Abel Ortega

    2007-10-01

    Full Text Available El Perú es un país endémico de Brucelosis, por lo que existe el riesgo de transmisión a través de sangre donada por no ser parte del tamizaje regular que realizan los bancos de sangre. Para conocer la prevalencia de anticuerpos contra Brucella en donantes del banco de sangre del Hospital Edgardo Rebagliati Martins, se analizó 1003 muestras de suero. La prueba tamiz fue Rosa de Bengala (RB; las muestras positivas fueron evaluadas por las pruebas de aglutinación en tubo (AT y 2-Mercaptoetanol (2-ME. Dos donantes fueron positivos a RB, confirmados por AT y 2-ME, se encontró una prevalencia de 0,20% (IC99%: 0,01-0,92. Se demostró la presencia de donantes portadores de anticuerpos contra Brucella con posible Brucelosis activa, evidenciándose la posibilidad de transmisión de esta infección. Se debe realizar mayores estudios con la finalidad de conocer la realidad de otros bancos de sangre.

  10. Prevalencia de anticuerpos IgG al toxoide tetánico en trabajadores sanitarios

    Directory of Open Access Journals (Sweden)

    B. Pérez Bermúdez

    2014-09-01

    Full Text Available Introducción: En el año 2009 el Ministerio de Sanidad y Consumo de España publica una nueva guía para la vacunación de la Difteria y Tétanos. Existen discrepancias con los tiempos de inoculación de las dosis de recuerdo con otros países y sociedades médicas. Material y métodos: Estudio descriptivo. Las variables analizadas fueron: IgG-Toxoide Tetánico, tiempo última dosis de vacunación, dosis totales, edad, sexo, hospital, habito tabáquico e índice de masa corporal. Se construyó un modelo de Regresión Logística. Con el fin de determinar los puntos de corte más sensibles y específicos para las variables temporales (tiempo desde la última dosis y edad se calcularon las curvas R.O.C Resultados: Las variables, edad O,R= 17,56 (1,840 - 167,760, dosis de vacuna inoculadas al trabajador, O,R= 0,064 (0,006 - 0,908 y tiempo de la última dosis de vacunación, O,R= 1,03 (1,017 - 1,047, son las variables significativas en el análisis multivariante. Los puntos de cortes, para la edad son 54 años (Sensibilidad S= 0,72, Especificidad SP=0,77 y 15 años tras la última dosis años (S= 0,88, SP=0,86, por encima de este corte la probabilidad de no tener anticuerpos al Tétanos es alta. Conclusiones: Ante un trabajador que refiere o documenta que hace más de 15 años de la ultima dosis deberíamos plantearnos la revacunación, si además este trabajador refiere no tener más de 5 dosis inoculadas y tiene más de 54 años la revacunación sería muy recomendable.

  11. Papel de los anticuerpos antiplaquetarios en la infección viral: una revisión sistemática de la literatura

    Directory of Open Access Journals (Sweden)

    Yenny M. Montenegro-Medina

    2011-04-01

    Conclusiones. Aunque la aparición de anticuerpos antiplaquetarios no se considera el único mecanismo que explica la trombocitopenia desarrollada en las infecciones virales mencionadas, de acuerdo con la literatura científica disponible, su presencia se asocia con la gravedad de la trombocitopenia y con potenciales implicaciones clínicas en los pacientes.

  12. RESULTS OF GENOTYPING HEPATITIS VIRUS B IN HBsAg-NEGATIVE BLOOD DONORS IN ASTANA, KAZAKHSTAN

    Directory of Open Access Journals (Sweden)

    Yu. V. Ostankova

    2017-01-01

    Full Text Available The prevalence of HBV infection is estimated by the frequency of occurrence of HBsAg and varies depending on the geographic region. Chronic infection is characterized by a stable presence of HBsAg for 6 months, with the exception of the occult form of the disease, characterized by the absence of HBsAg, an extremely low level of HBV DNA in the blood serum. The problem of identifying occult HBV (ocHBV is especially relevant because of the development of transplantology and transfusiology. However, serological screening of donor blood used in the Russian Federation and Central Asian countries does not reveal HBV seronegative donors. Since HBV infection is possible with the introduction of small doses of the virus, the importance of using complex molecular methods for detecting donor ocHBV is obvious, despite the low viral load, since donor blood is used predominantly in patients with severe course of various diseases characterized by increased susceptibility to HBV because of immunosuppression. The aim of our work was to study the characteristics of the genetic structure of the ocHBV in donors in Astana, Kazakhstan. A total of 500 blood plasma samples from HBsAg-negative donors were obtained in 2012 from residents of Kazakhstan, Astana. Using the method, we proposed to detect HBV DNA with a low viral load, HBV was detected in 9.4% of donors. Serological markers were found in 12.7% of patients with HBV DNA, 8.5% had HBcor IgG antibodies, 4.2% had HBcor IgG and HBe IgG antibodies at the same time. Thus, in 41 (87.3% of the blood donor, ocHBV was seronegative. Based on the phylogenetic analysis of the 47 isolates showed that the HBV of genotype D (95.75% prevails in the examined group in comparison with HBV of genotype A (4.25%. HBV subgenotypes are represented in the following ratios: D1 — 46.8%, D2 — 17.05%, D3 — 31.9%, A2 — 4.25%. In a comparative analysis, the distribution of HBV subgenotypes in the group with ocHBV and in the case of the

  13. Risk of hepatitis B reactivation in HBsAg-negative/HBcAb-positive patients with undetectable serum HBV DNA after treatment with rituximab for lymphoma: a meta-analysis.

    Science.gov (United States)

    Tang, Zilin; Li, Xiaodong; Wu, Shunquan; Liu, Yan; Qiao, Yan; Xu, Dongping; Li, Jin

    2017-09-01

    Hepatitis B surface antigen (HBsAg)-negative/hepatitis B core antibody (HBcAb)-positive patients with undetectable serum hepatitis B virus (HBV) DNA have experienced and resolved hepatitis B virus (HBV) infection. Lymphoma patients with resolved HBV infection have high risk of HBV reactivation when treated with robust immunosuppressive agents, but the reported rate varies extensively between different studies. This study aims to estimate the risk of HBV reactivation in HBsAg-negative/HBcAb-positive patients receiving rituximab-containing chemotherapy for lymphoma. Databases were searched for papers published in English until 8 August 2016. The pooled risk of HBV reactivation was estimated using a random-effects model. Data from 15 studies were retrieved, including a total of 1312 HBsAg-negative/HBcAb-positive lymphoma patients treated with rituximab-containing chemotherapy. The results revealed HBV reactivation rate of 9.0 % [95 % confidence interval (CI) 0.05-0.15]. In subgroup analysis, the reactivation rates for prospective and retrospective studies were 17 % (I 2  = 87.3 %; 95 % 0.08-0.39, p HBcAb-positive patients with rituximab treatment for lymphoma. Prophylactic use of anti-HBV agents should be seriously considered for such patients.

  14. Hepatitis B Virus Infection and Anti-HBc (Total Positivity in CKD Patients before Dialysis

    Directory of Open Access Journals (Sweden)

    Fareha Jesmin Rabbi

    2016-09-01

    Full Text Available Background: CKD patients are associated with HBV infection both as a cause and complication of treatment. CKD patients before starting dialysis therapy are considered as a high risk group because of impaired immune response compared with healthy individuals and also other risk factors related with treatment and management. Only HBsAg marker does not always follow the presence or absence of HBV infection. Anti-HBc (total alone positivity indicates previous exposure to HBV infection, window period and even after reactivation of resolved HBV infection. In some cases only anti-HBc positivity is interpreted as possible chronic low dose HBV infection (chronic carriage. Predialytic CKD patients were tested with three serological markers [HBsAg, anti-HBc (total and anti-HBs] for screening HBV infection. Proper diagnosis before dialysis and knowing the infection status would help both the patient and doctor to choose proper treatment approach. Objective: This cross-sectional study was done in the CKD patients before starting dialysis therapy to find out the HBV infection and to evaluate the infection by minimal serological markers as for screening. Materials and Methods: A total of 211 patients with chronic kidney disease stage five (CKD-V before starting dialysis therapy were included as subjects of this cross-sectional study. Among the CKD patients HBsAg was tested to see the prevalence. Other serological markers, i.e., anti-HBc (total and anti-HBs were tested in combination with HBsAg in 89 randomly selected patients among the subjects. The patients were also tested for anti-HCV to assess co-infection. After collecting all the data of different test results analyses were done by SPSS version 15.0. Results: Among total study population 10 (4.7% patients were found HBsAg positive. No patient was found positive for both HBsAg and anti-HCV. Among the 89 CKD patients only 2 (2.2% patients were HBsAg positive, and only one patient (0.9% was found positive

  15. Faster heme loss from hemoglobin E than HbS, in acidic pH: Effect ...

    Indian Academy of Sciences (India)

    We report studies on loss of heme at or below pH 3.0 from two clinically important hemoglobin variants, HbE and HbS, in the presence and absence of phopholipid membranes. The kinetics of heme loss has been studied at pH 3.0 to simulate the same at a faster rate than at physiological pH, for spectroscopic investigation.

  16. Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg

    OpenAIRE

    Marcondes,Jackson; Hansen,Ekkehard

    2008-01-01

    The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons wit...

  17. ANTICUERPOS DIRIGIDOS A PEQUEÑAS MOLÉCULAS DE NEUROTRANSMISORES Y PÉPTIDOS: PRODUCCIÓN Y APLICACIÓN DE ANTICUERPOS A DOPAMINA, SEROTONINA, GABA, VASOPRESINA, PÉPTIDO VASOACTIVO INTESTINAL, NEUROPÉPTIDO Y, SOMATOSTATINA Y SUSTANCIA P

    Directory of Open Access Journals (Sweden)

    Ruud M. Buijs

    2009-01-01

    Full Text Available En el presente trabajo se describe un procedimiento por medio del cual pueden ser obtenidos anticuerpos a partir de moléculas pequeñas de neurotransmisores usando glutaraldehido como agente acoplador. Este procedimiento fue desarrollado bajo la condición de que el inmunógeno usado para la preparación del anticuerpo fuera, hasta donde fuese posible, de la misma identidad que la molécula transmisora fijada al tejido. Con el de fin de lograr mayor éxito, los péptidos, aminas o aminoácidos1 fueron conjugados a una proteína acarreadora usando glutaraldehido. Los anticuerpos obtenidos fueron usados en estudios de inmunohistoquímica permitiendo la detección de estos transmisores después de un proceso de fijación con glutaraldehido o paraformaldehido para los péptidos.

  18. Prevalencia de anticuerpos contra Leptospira en personas asintomáticos y en perros de Chancay, Lima 2001

    Directory of Open Access Journals (Sweden)

    Manuel Céspedes

    2007-10-01

    Full Text Available Objetivos. Determinar la prevalencia de anticuerpos contra Leptospiras en personas asintomáticas dedicadas a la agricultura, pesca y comercio y en perros domésticos de localidades de Chancay (Huaral, Lima. Material y métodos. Estudio transversal analítico, se tomó muestras de suero de 268 pobladores de tres localidades, en quienes se evaluó la presencia de anticuerpos totales contra Leptospiras por el método de ELISA IgG y la prueba de microaglutinación (MAT. Se buscaron los factores asociados con la positividad a Leptospiras calculando el OR con su intervalo de confianza al 95%. De la misma manera, se tomó muestras de suero de 241 perros a los que se realizó la prueba de MAT. Resultados. La prevalencia de anticuerpos contra Leptospira en población asintomática fue de 10,1% (IC95%: 6,3-13,9, la cual estuvo asociada con el abastecimiento de agua para consumo en quebrada o pozo (OR: 3,48, IC95%: 1,39-8,74, con el antecedente de nadar en el río o acequia (OR: 4,75, IC95%: 1,51-14,92 y con tener una edad entre 21-40 años (OR: 2,47, IC95%: 1,10-5,52. Los serovares más frecuentes fueron Icterohaemorrhagiae y Canicola según MAT. En canes, 27,8% (67/241 tuvieron serología positiva a leptospiras. Conclusiones. Existe una mediana prevalencia de serología positiva para Leptospiras en la población general asintomática y condiciones favorables para la presencia de Leptospiras en las localidades estudiadas. En estas zonas se recomienda realizar actividades educativas preventivas frecuentes, tomando en cuenta los resultados de este estudio y el personal de salud debe sospechar de la leptospirosis como una causa de enfermedad febril.

  19. Prevalencia de anticuerpos séricos contra la bacteria Brucella sp. en una población humana tropical

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    Grettel Chanto

    2007-06-01

    Full Text Available La brucelosis es una enfermedad bacteriana generalizada con tendencia a la cronicidad si no se trata adecuadamente. El diagnóstico de laboratorio se realiza por el aislamiento e identificación del agente o en la mayoría de los casos mediante pruebas serológicas. En este estudio se informa la prevalencia de anticuerpos séricos contra Brucella sp. en habitantes de comunidades rurales de Cartago dedicadas principalmente a la ganadería de leche. Se analizó un total de 714 muestras representativas de la población mediante la técnica de aglutinación Rosa de Bengala en lámina (RBT y la prueba de aglutinación en microplaca (SAT. Se encontró una prevalencia de 0.87 %, y en ninguna persona seropositiva se demostró seroconversión. Aún, cuando el 71 % de la población refirió consumir leche no pasteurizada, no se encontró asociación estadísticamente significativa (1.09 %, IC 0.30-2.77 entre el consumo de leche no pasteurizada y la presencia de anticuerpos sugestivos de infección por Brucella sp. A pesar de ser una zona de alta prevalencia de brucelosis bovina por Brucella abortus, la ausencia de síntomas en la población y los bajos títulos de anticuerpos observados sugieren una escasa exposición previa al agente.Prevalence of serum antibodies for the bacterium Brucella sp. in a tropical human population. Brucellosis is a systemic bacterial disease that can last years if not treated properly. The laboratory diagnosis is made by serological tests or isolation of the agent. We studied the prevalence of antibodies against Brucella sp. in inhabitants of rural communities of Cartago, Costa Rica, who are dedicated mainly to dairy cattle activities. We analyzed 714 representative samples with the Rose Bengal plate agglutination test (RBT and the standard agglutination test in microplate (SAT. The overall prevalence was 0.87 %. No seroconversion was demonstrated for seropositive individuals. Even though 71 % of the population mentioned

  20. HBS-Tools for Hairpin Bisulfite Sequencing Data Processing and Analysis

    Directory of Open Access Journals (Sweden)

    Ming-an Sun

    2015-01-01

    Full Text Available The emerging genome-wide hairpin bisulfite sequencing (hairpin-BS-Seq technique enables the determination of the methylation pattern for DNA double strands simultaneously. Compared with traditional bisulfite sequencing (BS-Seq techniques, hairpin-BS-Seq can determine methylation fidelity and increase mapping efficiency. However, no computational tool has been designed for the analysis of hairpin-BS-Seq data yet. Here we present HBS-tools, a set of command line based tools for the preprocessing, mapping, methylation calling, and summarizing of genome-wide hairpin-BS-Seq data. It accepts paired-end hairpin-BS-Seq reads to recover the original (pre-bisulfite-converted sequences using global alignment and then calls the methylation statuses for cytosines on both DNA strands after mapping the original sequences to the reference genome. After applying to hairpin-BS-Seq datasets, we found that HBS-tools have a reduced mapping time and improved mapping efficiency compared with state-of-the-art mapping tools. The HBS-tools source scripts, along with user guide and testing data, are freely available for download.

  1. A Intervention Study to Improve HBsAg Testing and Preventive Practices for Hepatitis B in an Obstetrics Hospital

    Directory of Open Access Journals (Sweden)

    Ibrahim Koruk

    2011-06-01

    Full Text Available Objectives: This study was conducted to determine the effectiveness of a multicomponent intervention in Sanliurfa Gynecology and Obstetrics Hospital (SGOH, which is designed to overcome the obstacles encountered in the application of HBsAg testing to all delivering women, the administration of hepatitis B vaccine and Hepatitis B Immunglobulin(HBIG to newborns of HBsAg positive pregnant women. Methods: This is an intervention study, conducted between December 15, 2007 and December 30, 2008. Intervention procedure is composed of four methods; namely, motivation of all parties through academic leadership, implementation of workshops to determine the obstacles, provision of administrative commitment, and training of healthcare personnel. Results: In 2007, a total of 18,709 deliveries were realized in SGOH; HBsAg testing was performed in only 16.5% of the pregnant women and 0.8% of these women were found to be HBsAg positive. It was determined that HBIG was administered to 28.2% of newborns of HBsAg positive mothers and hepatitis B vaccine was given to 71.4% (n=13349 of all newborns. After the intervention, the increase in HBsAg test application status, HBsAg positivity, HIBG administration and hepatitis B vaccination was 6.0, 5.8, 2.0 and 1.2 fold, respectively (P<0.05. Conclusions: In order to reduce the incidence of hepatitis B and maintain patient safety in newborns, HBsAg testing should be included in routine pregnancy screening programs and this approach should be supported by legal arrangements and systematic continuous training activities. [TAF Prev Med Bull 2011; 10(3.000: 287-292

  2. Hepatitis B reactivation in HBsAg-negative/HBcAb-positive patients receiving rituximab for lymphoma: a meta-analysis.

    Science.gov (United States)

    Mozessohn, L; Chan, K K W; Feld, J J; Hicks, L K

    2015-10-01

    Patients with chronic hepatitis B (HBsAg-positive) are at risk of viral reactivation if rituximab is administered without antiviral treatment, a potentially fatal complication of treatment. Patients with so-called 'resolved hepatitis B virus infection' (HBsAg-negative/cAb-positive) may also be at risk. We performed a systematic review of the English and Chinese language literature to estimate the risk of hepatitis B virus (HBV) reactivation in HBsAg-negative/cAb-positive patients receiving rituximab for lymphoma. A pooled risk estimate was calculated for HBV reactivation. The impact of HBsAb status and study design on reactivation rates was explored. Data from 578 patients in 15 studies were included. 'Clinical HBV reactivation', (ALT >3 × normal and either an increase in HBV DNA from baseline or HBsAg seroreversion), was estimated at 6.3% (I(2) = 63%, P = 0.006). Significant heterogeneity was detected. Reactivation rates were higher in prospective vs retrospective studies (14.2% vs 3.8%; OR = 4.39, 95% CI 0.83-23.28). Exploratory analyses found no effect of HBsAb status on reactivation risk (OR = 0.083; P = 0.151). Our meta-analysis confirms a measurable and potentially substantial risk of HBV reactivation in HBsAg-negative/cAb-positive patients exposed to rituximab. However, heterogeneity in the existing literature limits the generalizability of our findings. Large, prospective studies, with uniform definitions of HBV reactivation, are needed to clarify the risk of HBV reactivation in HBsAg-negative/cAb-positive patients. © 2015 John Wiley & Sons Ltd.

  3. [Early physical growth and disease analysis among children born delivered by HBsAg-positive mothers].

    Science.gov (United States)

    Zhang, X H; Wang, Q; Zheng, W; Li, X H; Jiang, Q Q; Zhou, C F; Qiu, L Q

    2017-06-06

    Objective: To estimate the early physical growth and disease in children born to HBsAg-positive mothers. Methods: This was a retrospective cohort study. Three areas as Xihu in Hangzhou, Lanxi in Jinhua, and Haiyan in Jiaxing in Zhejiang province were selected by cluster sampling. The growth outcomes of children born to HBsAg-positive mothers (exposure group) and matched 1∶1 women uninfected with HBV (control group) in 2014 were investigated and compared at birth, 6, 9, 12, and 18 months, respectively. There were totally 342 children in each group. Results: The incidences of low birth weight (LBW) for children born to exposure and control group were 1.8% (6/342), and 2.6% (9/342), respectively ( P= 0.433); and, rates of preterm birth were 2.3% (8/342), and 2.0% (7/342), respectively ( P= 0.794). The mean birth weight of children born to mothers without HBV infection (3.4±0.4) kg was dramatically higher than children in exposure group (3.3±0.4) kg ( P= 0.019). At 18 months, the average head circumference was significantly greater among children in control group (47.3±1.3) cm than children in exposure group (47.0±2.0) cm ( P= 0.038). Additional, mean birth weeks, height, weight, increases in height/weight/head circumference each month, weight/height/head circumference for age Z scores, proportion of growth retardation and low weight, disease prevalence were not observed statistically differences between two groups ( P> 0.05). All children born to HBsAg-positive mothers were received three-dose HBV vaccination. The rate of hepatitis B immunoglobulin for births born to HBsAg-positive was 98.8% (338/342). Mother to children transmission of HBV at 18 months was 1.0% (1/97). Conclusion: No significant differences in growth development and disease prevalence were found among children born to HBsAg-positive women and women without HBV infection.

  4. Determination of Relative Frequency of HBS Ag, HCV and HIV Antibodies Serum Markers among Admitted Intravenous Drug Users in Infectious Disease Ward of Razi Hospital in Ahvaz, 2004-2005

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    Abdolrasool Nikkhooy

    2012-07-01

    Full Text Available Introduction: Intravenous drug users as a serious health problem in communities have economical and social effects as well as health and hygienic complications. Viral infections may be transmitted through drug injection by shared syringes among users. The aim of this study has been to determine the relative frequency of HBV, HCV and HIV infection’s markers as epidemiological data in Ahvaz. Materials & Methods: This retrospective cross sectional study was conducted on IV drug users (IVDUs who were admitted in infectious diseases ward of Razi Ahvaz Hospital in 2004-2005. The collected data of serum markers of these patients were coded, and statistical analyses were conducted. Results: 1890 patients were evaluated and 258 patients were IVDUs (14.6%. 154 patients (59.98% were tested for anti HCV-Ab of whom 65 patients were HCV-Ab positive (42.2%. 205 patients (79.45% were tested for anti HIV-Ab of whom 38 patients were HIV-Ab positive (18.53%. 67 patients (25.96% were tested for HBs-Ag of whom 15 patients were HBs-Ag positive (22.67%. 12 patients (4.65% were tested for anti HBc-Ab of whom 8 patients were HBc-Ab positive (66.66%. Conclusion: In this study, high infection rate relates to different causes such as increasing consumes of opium substances and recent differences in fumigated opium substances pattern toward injecting drug use in society level, which increases the prevalence of these infections, The present study determined some critical information about the prevalence of serum markers HBS Ag, HCV and HIV antibodies among intravenous drug users in southwestern of Iran.

  5. Evolución serológica en mujeres con anticuerpos antifosfolípido positivos

    OpenAIRE

    Daroca Bengoa, Germán

    2015-01-01

    En el presente trabajo se estudió de forma retrospectiva una cohorte de 90 mujeres con positividad confirmada de anticuerpos antifosfolípido (aPL), de acuerdo con los criterios diagnósticos de síndrome antifosfolípido (SAF). Las pacientes fueron clasificadas en 3 grupos: SAF primario (n=40), aPL positivos sin cumplir el criterio clínico de SAF (n=38) y lupus eritematoso sistémico (LES) con aPL positivos (n=12). Después de 119,4±33,0 meses de seguimiento, un 60,0% de las pacientes mostró negat...

  6. Advanced characterization of MIMAS MOX fuel microstructure to quantify the HBS formation

    Energy Technology Data Exchange (ETDEWEB)

    Bouloré, Antoine, E-mail: antoine.boulore@cea.fr [CEA, DEN, DEC Fuel Research Department, Cadarache, F13108 Saint-Paul-lez-Durance (France); Aufore, Laurence; Federici, Eric [CEA, DEN, DEC Fuel Research Department, Cadarache, F13108 Saint-Paul-lez-Durance (France); Blanpain, Patrick [AREVA NP SAS, 10 rue Juliette Récamier, F-69456 Lyon (France); Blachier, Rémi [EDF, SEPTEN, 12-14 Av. Dutrievoz, F-69628 Villeurbanne (France)

    2015-01-15

    Highlights: • An advanced characterization of MIMAS MOX fuel based only on fresh fuel pellet characterization. • A probabilistic approach to model the High Burnup Structure formation in oxide fuels. • Validation of the method by comparing to experimental data obtained on fuel irradiated in the Halden reactor. - Abstract: Fission gas behaviour in accidental situations is closely related to the location of fission gas before the accident. More precisely, most of the fission gas in intergranular position is released during the accident and HBS zones contribute a lot to this intergranular quantity. So a methodology to characterize the HBS zones a priori from examination of unirradiated pellet has been developed at CEA. Characterization of plutonium distribution in MIMAS MOX fresh fuel pellets can be performed by image analysis on 1 mm{sup 2} X-ray mappings of plutonium acquired using Electron Probe Micro Analysis (EPMA). The specific software developed to describe the fuel using Pu X-ray mapping (ANACONDA) has been improved in order to simulate the fission products (FP) production and recoil during a given irradiation of the fuel, taking into account the evolution of the plutonium due to neutron irradiation. This simulation results from calculations with our fuel performance code ALCYONE combined with image processing. The final result is a mapping of local burn-up, but also the distribution of the relative FP concentration as a function of the local burn-up. A validation of this simulation process has been done by comparing the simulated mapping of neodymium to one measured on the same fuel batch after irradiation. Using previous studies of mechanisms for HBS formation, a probabilistic criterion for HBS formation has been proposed, based on the EPMA measurements of the decrease of the xenon signal as a function of the local burn-up. Combining the simulated FP cartography with this probabilistic HBS formation criterion, it is possible to calculate the surface

  7. Clinical Characteristics and Correlation Analysis of Subjects with Chronic Hepatitis B Virus (HBV) Infection and Sustained Low Levels of Hepatitis B Surface Antigen (HBsAg)

    Science.gov (United States)

    Cheng, Jun; Dai, Yuzhu; Yan, Li; Zhou, Huajun; Xu, Xujian

    2018-01-01

    Background The aim of this study was to investigate the clinical characteristics of individuals with chronic hepatitis B virus (HBV) infection with persistent low levels of hepatitis B surface antigen (HBsAg) and to undertake a correlation analysis of the clinical characteristics. Material/Methods The study included 1,204 subjects with chronic HBV infection. Serum HBsAg, HBV envelope antigen (HBeAg), and HBV core antigen (HBcAg) levels were measured using the chemiluminescent microparticle immunoassay (CMIA) and the neutralization test. HBV DNA was measured using real-time fluorescence quantitative polymerase chain reaction (RT-FQ-PCR). Results There were 1,023 subjects in the high-level HBsAg group (HBsAg level ≥10 IU/mL) and 181 subjects in the low-level HBsAg group (HBsAg level HBV-M2), and the asymptomatic carrier (ASC) status was 98.34%. The low-level HBsAg group had a lower HBV DNA-positive rate compared with the high-level HBsAg group (40.33% vs. 75.07%), with a normal distribution across all age groups (P>0.05). The low-level HBsAg group included an older age group. A low-level of HBsAg was positively correlated with a low level of replication of HBV DNA (r=0.452). Conclusions The findings of this study showed that individuals with chronic HBV infection and sustained low-levels of HBsAg were an older population and had a lower level of replicating HBV DNA when compared with individuals with high levels of HBsAg, and the majority (93.7%) were also HBsAg and HBeAg and HBcAg-positive. PMID:29593208

  8. Lamivudine monoprophylaxis for de novo HBV infection in HBsAg-negative recipients with HBcAb-positive liver grafts.

    Science.gov (United States)

    Vizzini, Giovanni; Gruttadauria, Salvatore; Volpes, Riccardo; D'Antoni, Adele; Pietrosi, Giada; Filì, Daniela; Petridis, Ioannis; Pagano, Duilio; Tuzzolino, Fabio; Santonocito, M Maria; Gridelli, Bruno

    2011-01-01

    We followed the efficacy of long-term lamivudine monotherapy in preventing development of de novo hepatitis B (DNHB) in a large cohort of hepatitis B surface antigen (HBsAg)-negative recipients with grafts from hepatitis B core antibody (HBcAb)-positive donors. Recipients were observed over a long follow-up. Between July 1999 and December 2008, 45 patients (median age 54, range 19-67) who were HBsAg negative before transplantation were included in the study of monoprophylaxis with lamivudine starting on post-operative day 1, and continuing for life. Mean follow-up: 37.9 months; median 32.1 months (range 2.4-117). No suspension of therapy was reported during the study. Post-transplantation, no DNHB was observed in follow-up: all 45 HBsAg-negative recipients remained HBsAg and HBV DNA negative. Thirty-four of these HBsAg-negative recipients were alive at conclusion of the study. A total of 11 patients died, five of HCV recurrence, two of hepatocellular carcinoma (HCC) recurrence, two of disseminated KSV infection, and two of multiorgan failure because of early graft dysfunction. Patient and graft survival of HBsAg-negative recipients with HBcAb-positive donor grafts (45 cases) were not significantly different from those of the HBsAg-negative recipients with HBcAb-negative donor grafts (302 cases). In our experience, lamivudine monoprophylaxis provided complete protection against HBV reactivation and showed long-term efficacy. © 2010 John Wiley & Sons A/S.

  9. [Comparative analysis of liver function in HBsAg-/HBcAb+ patients with diffuse large B-cell lymphoma treated with CHOP and R-CHOP regimens].

    Science.gov (United States)

    Huang, Yan-hua; He, Xiao-hui; Qin, Yan; Yang, Sheng; Lü, Zheng; Dong, Mei; Zhou, Sheng-yu; Liu, Peng; Zhang, Chang-gong; Yang, Jian-liang; Shi, Yuan-kai

    2012-05-01

    To analyze the liver function in patients with diffuse large B-cell lymphoma(DLBCL), who are hepatitis B surface antigen negative/antibody to hepatitis B core antigen positive (HBsAg-/HBcAb+), treated with CHOP and R-CHOP regimens. In this retrospective study, 86 DLBCL patients, who were HBsAg-/HBcAb+, were collected from Cancer Hospital of Chinese Academy of Medical Sciences between January 2005 and December 2008. The patients were given at least two cycles of chemotherapy using CHOP-like or R-CHOP-like regimen without anti-HBV treatment, and followed-up for at least 12 months after completion of therapy. Forty-seven patients received CHOP-like regimen while 39 patients received R-CHOP-like regimen. There were no significant differences in the degree of liver dysfunction between CHOP group and R-CHOP group after the 1st, 2nd, 3rd, 4th and 6th cycles (22.7% - 46.7% with CHOP and 17.6% - 34.2% with R-CHOP, respectively, (all P > 0.05), except for the 5th cycles (28.6% vs. 6.2%, P = 0.026). Liver function in most patients in CHOP group and R-CHOP group was normal after every cycle (53.3% - 77.3% and 65.8%-93.8%, respectively). Meanwhile, there were no significant differences in the degree of liver dysfunction between CHOP group and R-CHOP group in the 1st-3rd month, 4th-6th month, 7th-9th month and 10th-12th month after completion of therapy (7.7% - 40.0% with CHOP and 7.4% - 32.0% with R-CHOP, respectively, all P > 0.05). The present study reveals a low incidence of liver dysfunction in HBsAg-/HBcAb+ DLBCL patients, both in CHOP group and in R-CHOP group. It may indicate a potential low incidence of HBV reactivation in these groups, and Rituximab do not increase the rate of liver dysfunction. Therefore, these data may not support regularly prophylactic antiviral therapy during chemotherapy, but close monitoring of liver function, HBV serum markers and HBV DNA level are demanded.

  10. Serological profile of incidentally detected asymptomatic HBsAg positive subjects (IDAHS)

    International Nuclear Information System (INIS)

    Khokhar, N.; Gill, M.L.

    2004-01-01

    Objective: To evaluate the serological profile of patients with incidentally detected positive hepatitis-B surface antigen (HBsAg) and to asses the risk factors. Design: An observational study. Place and Duration of Study: This study was conducted at Shifa International Hospital, Islamabad from 1999 to 2003. Patients and Methods: All patients who presented to gastroenterology clinic of Shifa Intentional Hospital, Islamabad with positive HBsAg, detected incidentally, were tested for alamine transaminase (ALT), hepatitis Beantigen (HBeAg) and in certain cases hepatitis-B virus DNA (HBV DNA) by polymerase chain reaction (PCR). Their risk factors for acquisition of infection were assessed with specific questions. Results: A total of 224 patients were examined. One hundred sixty-four (73.2%) were male and 60 (26.8%) female. Mean age of all the subjects was 32.45 plus minus 11.85 years. Out of 224 patients, 48 (21.4%) were positive for HBeAg and 176 (78.6%) were negative. Out of 48 subjects who were positive for HBeAg, 36 underwent HBV DNA determination and 32 (88.8%) were positive for HBV DNA. Out of 176 subjects who had negative HBeAg, 46 had elevated ALT and in those HBV DNA was performed and 14 had positive HBV DNA. Most common risk factors detected in these patients were intramuscular injections and surgery, however, in a large number, risk factors were unknown. Conclusion: Twenty-one percent asymptomatic subjects with positive HBsAg were found to be HBeAg positive. A large number of subjects with negative HBeAg had HBV DNA positive suggesting presence of precore mutants. Intramuscular injections and surgery were noted to be frequent risk factors in these subjects. (author)

  11. Expression of Hepatitis B virus surface antigen (HBsAg from genotypes A, D and F and influence of amino acid variations related or not to genotypes on HBsAg detection

    Directory of Open Access Journals (Sweden)

    Natalia M. Araujo

    Full Text Available The impact of hepatitis B virus (HBV genotypes on the sensitivity of surface antigen (HBsAg detection assays has been poorly investigated. Here, plasmids carrying consensus or variant coding sequences for HBV surface proteins from genotypes A, D and F, were constructed. HBsAg levels were evaluated in medium and extracts of transfected CHO cells by a commercial polyclonal-based assay. We show that HBsAg detection values of consensus forms from genotypes D and F were, respectively, 37% and 30% lower than those obtained by genotype A. However, the presence of two single variations, T143M in genotype A, and T125M in genotype D, produced a decrease of 44% and an increase of 34%, respectively, on HBsAg mean values in comparison with their consensus forms. In conclusion, HBsAg detection levels varied among HBV genotypes. However, unique amino acid substitutions not linked to genotypes, such as T125M and T143M described here, should have more implications in HBV immunological diagnostics than the set of variations characteristic of each HBV genotype.

  12. Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg

    Directory of Open Access Journals (Sweden)

    Jackson Marcondes

    Full Text Available The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L. using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety "Vitória de Verão" genetically modified.

  13. Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg.

    Science.gov (United States)

    Marcondes, Jackson; Hansen, Ekkehard

    2008-12-01

    The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety 'Vitória de Verão' genetically modified.

  14. Hepatitis B reactivation in HBsAg-negative/HBcAb-positive patients receiving immunosuppressive therapy for glomerulonephritis: a retrospective analysis.

    Science.gov (United States)

    Fang, Jing; Li, Wenge; Peng, Xiangxin; Tan, Zhao; Tan, Min; Zhang, Cong; Wang, Wenbo; Xu, Zhihong; Zhou, Gumin

    2017-03-01

    The aim of this study is to investigate the prevalence and risk factors for hepatitis B virus (HBV) reactivation in HBsAg-negative/HBcAb-positive patients receiving immunosuppressive therapy for glomerulonephritis. We performed a retrospective study of 745 HBsAg-negative/HBcAb-positive patients undergoing immunosuppressive therapy for glomerulonephritis from years 2003 to 2012 at the department of nephrology, China-Japan Friendship Hospital, Beijing, China. The patients were divided into HBV reactivation group (n = 27) and non-HBV reactivation group (n = 718). The prevalence of HBV reactivation in patients receiving immunosuppressive therapy for glomerulonephritis was up to 3.62% in serological status of HBsAg-negative/HBcAb-positive. HBV reactivation was associated with several findings: greater proportion of lupus nephritis (25.93 vs. 9.61%, p = 0.014), much higher percentage of HBsAb-negative (74.07 vs. 23.82%, p HBcAb-positive glomerulonephritis patients treated with immunosuppressant, and the prevalence was up to 3.62%. Patients with serological status of HBsAb-negative, more than 1 year of immunosuppressive therapy, and combined immunosuppressant are independent risk factors for HBV reactivation.

  15. Detección de Anticuerpos IgG Específicos para Sarampión mediante la Técnica de Inmunofluorescencia Indirecta

    Directory of Open Access Journals (Sweden)

    Mónica Nieto Z

    2000-01-01

    Full Text Available La técnica de ínmunofluorescencia indirecta para detectar anticuerpos IgG específicos a sarampión (IFI-IgG fue evaluada con la prueba de neutralización por reducción de placas (PRN con 128 muestras de suero de personas con edades entre 0 y 15 años, 64 con antecedente de vacunación y 64 sin éste antecedente. El IFI-IgG alcanzó una sensibilidad de 80,4% y una especificidad de 100,0%, mostrando ser una prueba sencilla, reproducible, y tener correlación con PRN, aunque menos sensible que ésta última, especialmente cuando la PRN estimó títulos bajos de anticuerpos. Se concluye que la técnica IFI-IgG puede ser usada como método de rutina para la confirmación de sarampión en muestras de fase aguda y convalesciente de la enfermedad (sueros pareados, así como para estudios de prevalencia de anticuerpos en la comunidad, aunque no reemplazaría a la PRN.

  16. Anticuerpos frente a virus West nile y otros virus transmitidos por artropodos en la poblacion del Delta del Ebro

    Directory of Open Access Journals (Sweden)

    Lozano Alvaro

    1998-01-01

    Full Text Available FUNDAMENTOS: El virus West Nile (VWN es un Flavivirus que se transmite al hombre a través de distintas especies de mosquitos y produce brotes y casos esporádicos de enfermedad en distintas regiones del Viejo Mundo, incluída la Cuenca Mediterránea. Las zonas húmedas europeas que acogen aves migratorias procedentes de África constituyen áreas de alto riesgo para esta infección, así como para otras infecciones víricas transmitidas por artrópodos. MÉTODOS: Con objeto de investigar la prevalencia de la infección por el VWN y otros virus de transmisión similar en la población humana del Delta del Ebro, se estudiaron 1037 muestras de suero, obtenidas en 10 localidades de la zona, para presencia de anticuerpos frente a VWN y otros 12 virus transmitidos por artrópodos (3 Alfavirus, 8 Flaviviridae y 1 Bunyaviridae mediante titulación por inhibición de la hemaglutinación (IHA. En algunos casos se estudió la presencia de IgM específica por IHA tras fraccionar el suero por centrifugación en gradientes de sacarosa. RESULTADOS: En total, se encontró reactividad significativa frente a alguno de los virus probados en 130 casos (12.5%; 4.1% frente a Alfavirus, 8.0% frente a Flaviviridae y 0.4% frente a Bunyaviridae. El análisis de los títulos de anticuerpos reveló porcentajes significativos de muestras con títulos elevados frente a antígenos de VWN y otros. La distribución de la seroprevalencia fue muy desigual, concentrándose fundamentalmente en 3 localidades del interior del Delta (Ampolla, San Jaime y Montells, donde la prevalencia de anticuerpos frente a Flaviviridae llegó a alcanzar el 30% y se observaron niveles residuales de IgM frente a VWN en algunos sueros. CONCLUSIONES: Estos resultados y los obtenidos previamente en otras regiones de la Península Ibérica sugieren que el VWN circula en la población humana de las zonas de riesgo y produce brotes epidémicos periódicos. Habida cuenta del alto porcentaje de

  17. Evidenciação de antígeno da hepatite B (HBsAg em "manchas" obtidas de exemplares de Triatominae: nota prévia Hepatitis B antigen (HBsAg in wildcaught Triatominae in Brazil: communication

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    J. A. N. Candeias

    1976-09-01

    Full Text Available De 890 "manchas" provenientes de insetos dos gêneros Panstrongylus, Rhodnius e Triatoma, positivas para sangue humano, 7 deram resultados presumptivamente positivos por hemaglutinação passiva para HBsAg. Deste total só um caso foi confirmado como positivo, por RIE (radioimunoensaio, correspondendo a uma "mancha" obtida de Triatoma sordida (ninfa de 5.º estádio.A total of 890 impressions on filter paper identified as containing human blood, were examined for the presence of hepatitis B antigen (HBsAg, using the Hepanosticon haemagglutination and radioimmunoassay techniques. The samples examined belong to the Panstrongylus, Rhodnius and Triatoma genres. The Hepanosticon haemagglutination technique showed that 7 impressions, after elution, were presumably positive for HBsAg, but by radioimmunoassay, this antigen could only be detected in 1. The positive sample was obtained from a specimen of Triatoma sordida (fifth instar nymph.

  18. Impact of HBV genotype and mutations on HBV DNA and qHBsAg levels in patients with HBeAg-negative chronic HBV infection.

    Science.gov (United States)

    Kuhnhenn, L; Jiang, B; Kubesch, A; Vermehren, J; Knop, V; Susser, S; Dietz, J; Carra, G; Finkelmeier, F; Grammatikos, G; Zeuzem, S; Sarrazin, C; Hildt, E; Peiffer, K-H

    2018-04-10

    HBV DNA and quantitative (q)HBsAg levels as prognostic markers for HBV-related disease are mostly validated in Asia and their significance in Western populations is uncertain. To analyse the impact of the HBV genotype and frequent mutations in precore (PC), basal core promoter (BCP) and preS on HBV DNA and qHBsAg levels. HBV DNA and qHBsAg serum levels of 465 patients with HBeAg-negative chronic HBV infection were correlated with the HBV genotype and mutations in PC, BCP and preS. For a detailed analysis of the molecular virology, genotype A2 genomes harbouring these mutations were analysed for replication efficacy and HBsAg release in cell culture. While no impact of the HBV genotype on HBV DNA levels was observed, qHBsAg levels differed up to 1.4 log among the genotypes (P HBV DNA levels (P HBV genome harbouring a preS deletion. In contrast, a perinuclear HBsAg accumulation was detected for the PC and BCP-variants, reflecting an impaired HBsAg release. qHBsAg serum levels depend on the HBV genotype and together with HBV DNA levels on frequent mutations in PC, BCP and preS in HBeAg-negative patients. qHBsAg cut-offs when used as prognostic markers require genotype-dependent validation. © 2018 John Wiley & Sons Ltd.

  19. Spontaneous HBsAg loss in Korean patients: relevance of viral genotypes, S gene mutations, and covalently closed circular DNA copy numbers

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    Kyun-Hwan Kim

    2014-09-01

    Full Text Available Background/AimsOccult HBV infection can persist following HBsAg loss and be transmitted, but the virological features are not well defined.MethodsHere we investigated 25 Korean patients who lost HBsAg during follow up, either spontaneously or subsequent to therapy.ResultsWhereas subtype adr (genotype C was found in 96% of HBsAg positive patients, 75 % of patients who lost HBsAg spontaneously were seemed to be infected with the ayw subtype with sequence similar to genotype D. Mutations in the major hydrophilic region (MHR of HBsAg were found in 7 patients who lost HBsAg spontaneously. The mutations include T123S, M125I/N, C139R, D144E, V177A, L192F, and W196L, some of which have not been reported before. Functional analysis via transfection experiments indicate that the C139R and D144E mutations drastically reduced HBsAg antigenicity, while the Y225del mutation found in one interferon-treated patient impaired HBsAg secretion.ConclusionsLack of detectable HBsAg in patient serum could be explained by low level of ccc DNA in liver tissue, low antigenicity of the surface protein, or its secretion defect.

  20. Features of the electronic structure of the active center of an HbS molecule

    Science.gov (United States)

    Novoselov, D. Yu.; Korotin, Dm. M.; Anisimov, V. I.

    2016-01-01

    Features of the electronic structure of the nonprotein part of the mutant form of the human hemoglobin molecule, HbS, are studied along with the magnetic state of the iron ion that is the "nucleus" of the active center of the molecule. It is found that the mutant form of the HbS molecule differs from a normal hemoglobin molecule by the distortion of the local environment of the iron ion, which changes the energy level splitting by a crystal field. As a result of ab initio calculations, the magnetic transition in the iron atom from the high-spin state to the low-spin state upon the addition of molecular oxygen to hemoglobin molecule is reproduced. It is established for the first time that a change in the crystal and electronic structure of the active center as a result of a mutation can lead to a substantial change in the energy of the bond between the active center of the hemoglobin molecule and an oxygen molecule.

  1. Teniosis y detección de anticuerpos anticisticerco en personas de una comunidad rural del estado de Guerrero

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    Martínez-Maya José Juan

    2003-01-01

    Full Text Available OBJETIVO: Determinar la frecuencia de portadores de Taenia sp. y su relación con el diagnóstico de cisticercos en humanos en una comunidad rural del estado de Guerrero, México. MATERIAL Y MÉTODOS: Para detectar portadores de Taenia sp. se analizaron 403 muestras de heces de personas, por medio de ELISA para coproantígenos de Taenia sp., así como 92 muestras de suero para detectar anticuerpos anticisticerco mediante inmunoelectrotransferencia. El diseño del estudio fue transversal y se llevó a cabo durante 1998. Se hizo estadística descriptiva y se estimó razón de momios. RESULTADOS: De 403 muestras de heces evaluadas, cinco resultaron positivas (1.2 %. Sólo en dos de las cinco personas positivas se obtuvo el cestodo adulto. En 3 (3.26% de los 92 sueros se encontraron anticuerpos anticisticerco. Del total de sueros, 17 fueron de las personas con diagnóstico positivo a teniosis por coproantígenos o que cohabitaban con ellos (primer grupo, los restantes 75 provenían de personas en quienes no se detectaron casos en las viviendas (segundo grupo. En el primer grupo se detectaron 2 (11.8% sueros positivos, mientras que en el segundo sólo 1 (1.3% (RM= 9.87, I.C 0.64-295.56, p= 0.086. CONCLUSIONES: La dificultad para obtener el parásito adulto en las personas positivas a coproantígenos puede deberse a características propias de éste que dificultan su expulsión, a que la permanencia del cestodo en su huésped es menor a la esperada o a que el tratamiento fue insuficiente para obtener el parásito, o bien, a problemas de especificidad de la prueba. Es necesario realizar estudios tendientes a evaluar estas posibilidades, lo cual permitiría conocer mejor la dinámica de transmisión de esta parasitosis, con el fin de establecer medidas de prevención y control, además de poder comparar con mayor veracidad la eficacia de las pruebas diagnósticas en condiciones de campo.

  2. Results of HBsAg examination with immunoelectrophoresis and radioimmunoassay in VPR, Laotian PDR and Cuban R

    Energy Technology Data Exchange (ETDEWEB)

    Michal, L.; Szilagyiova, M.; Zahradny, V.; Holan, J.; Krajnak, V. (Komenskeho Univ., Martin (Czechoslovakia). Lekarska Fakulta); Padurova, J. (Okresna Hygienicka Stanica, Zilina (Czechoslovakia))

    1984-06-21

    The results are compared of examining 112 Vietnamese, 30 Laotian and 50 Cuban citizens for HBsAg using IEP and RIA methods. Of the examined persons with no liver disorders HBsAg was found in 23 (31.5%) of the Vietnamese and 1 (2.5%) of the Cubans using the RIA method and only in 4 (5.5%) of the Vietnamese using the IEP method. Of the examined persons with liver disorders RIA found HBsAg in 12 (30.8%) of the Vietnamese, 5 (71.4%) of the Laotians and 2 (20.0%) of the Cubans while with IEP only in 3 (7.7%) Vietnamese 2 (28.6%) Laotians and 1 (10.0%) of the Cubans examined.

  3. Seroprevalence of hepatitis B e antigen (HBe antigen and B core antibodies (IgG anti-HBcore and IgM anti-HBcore among hepatitis B surface antigen positive blood donors at a Tertiary Centre in Nigeria

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    Akinbami Akinsegun A

    2012-03-01

    Full Text Available Abstract Background Hepatitis B virus (HBV is a common cause of liver disease throughout the world. HBV is transmitted through blood and other body fluids, including semen and saliva. Chronic replication of HBV virons is characterized by persistence circulation of HBsAg, HBeAg and HBV DNA; usually with anti-HBc and occasionally with anti-HBs. Aim: To determine the prevalence of HBeAg, IgG anti-HBcore and IgM anti-HBcore amongst HBsAg positive blood donors. These parameters are reflective of transmissibility and active hepatitis B infection. A cross sectional study was carried out at the blood donor clinics of Lagos State University Teaching Hospital Ikeja and Lagos University Teaching Hospital Idiaraba. A total of 267 donors were recruited to determine HBe antigen, IgG and IgM anti-HBcore antibodies amongst hepatitis BsAg positive donors. Five milliliters of blood was collected from those who tested positive to HBsAg screen during donation. The sera were subjected to enzyme linked immunosorbent assay (ELISA. Pearson chi-squared test was used for the analytical assessment. Findings A total number of 267 HBsAg positive blood donors were studied. A seroprevalence of 8.2% (22 of 267 HBeAg was obtained, 4 of 267 (1.5% were indeterminate while 241 (90.3% tested negative. Only 27 out of 267 donors (10.1% tested positive to IgM anti-HBcore, 234(87.6% tested negative, while 6(2.2% were indeterminate. A higher percentage of 60.7% (162 of 267 tested positive to IgG anti-HBcore, while 39.3% (105 of 267 tested negative. Conclusion There is a low seroprevalence rate of HBeAg-positive chronic hepatitis and relatively high IgG anti-HBcore and IgM anti-HBcore rates in South West Nigeria.

  4. Recidiva de vasculitis asociada a anticuerpos anticitoplasma de neutrófilos en un paciente con trasplante renal

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    Pedro García Cosmes

    2016-03-01

    Full Text Available La afectación renal de las vasculitis asociadas a anticuerpos anticitoplasma de neutrófilos (ANCA puede conducir a enfermedad renal crónica con necesidad de tratamiento renal sustitutivo. En estos enfermos el trasplante renal ofrece excelentes tasas de supervivencia del injerto y del receptor a largo plazo, por lo que pueden ser trasplantados cuando la enfermedad está en remisión. Sin embargo, la amenaza de recidivas de la enfermedad en el injerto se mantiene, aunque, con las modernas pautas de inmunosupresión, su incidencia es menor. Presentamos el caso de un varón diagnosticado de glomerulonefritis extracapilar tipo III C-ANCA (+ que desarrolló una recidiva de la enfermedad en el injerto renal 8 años después de ser trasplantado. La intensificación de la inmunosupresión con plasmaféresis consiguió controlar la enfermedad.

  5. Frequency of anti-HCV, Hbsag and related risk factors in pregnant women at Nishtar Hospital, Multan

    International Nuclear Information System (INIS)

    Taseer, I.H.; Ishaq, F.; Hussain, L.; Safdar, S.; Mirbahar, A.M.; Faiz, S.A.

    2010-01-01

    Background: Viral hepatitis is a global issue. Among the hepatitis viruses hepatitis B and C are important in South Asia including Pakistan. There are various modes of transmission of these viruses. Vertical transmission is also gaining importance. Antepartum screening for HBV and HCV would help the infected women for appropriate antiviral therapy at appropriate time as well as for taking proper care of the newborns. The present study was designed to see the frequency of HBsAg and anti-HCV in pregnant women at Nishtar Hospital, Multan. Methods: This was a cross-sectional study carried out using non-probability purposive sampling technique. The period of the study was from June 2006 to August 2007. Five hundred (500) pregnant women attending outpatient department of Gynaecology and Obstetrics were included. Informed consent was taken. A specially designed proforma was filled in. Anti-HCV and HBsAg were tested by device method. Data were analyzed on SPSS-11. Results: Out of 500 pregnant women 35 (7.00%) were found to be anti-HCV positive and 23 (4.60%) were positive for HBsAg. Mean age was 26.7+-4.8 years. Majority of the patients 263 (52.60%) were in the age group 26-35 years. 138 (27.60%) women were nulliparous and 282 (56.40%) were para 1-4 and anti-HCV and HBsAg were common in this parity group. Only 80 (16.00%) women were para 5 or more. All anti-HCV and HBsAg positive women were house-wives. Most of them were belonging to rural areas having poor socio-economic status. Among 35 anti-HCV positive women, 20 (57.14%) had history of previous surgery, while 13 (37.14%) had history of multiple injections, 5 (14.28%) received blood transfusion, 4 (11.42%) had ear/nose piercing while tattooing was seen in only 2 (5.71%). Among 23 HBsAg positive women, 10 (43.47%) had history of previous surgery. History of multiple injections was present in 6 (26.08%) patients, 4 (17.39%) patients had history of blood transfusion, tattooing, ear/nose piercing, history of dental

  6. Bakens verzetten in het voortgezet onderwijs, 1863-1920 : Gymnasium, h.b.s. en m.m.s. in onderwijssysteem, leerplan en geschiedenisonderwijs

    NARCIS (Netherlands)

    Amsing, H.T.A.

    2002-01-01

    This thesis focuses on the history of four different types of Dutch secondary school: the gymnasium (with classical languages), the HBS-5 (secondary modern school with a five-year course), the HBS-3 (secondary modern school with a three-year course) and the MMS (secondary school for girls). The main

  7. Incidence and clinical characteristics of hepatitis B virus reactivation in HBsAg-negative/HBcAb-positive patients receiving rituximab for rheumatoid arthritis.

    Science.gov (United States)

    Tien, Ya-Chih; Yen, Hsu-Heng; Chiu, Ying-Ming

    2017-01-01

    To analyse the incidence, clinical characteristics, and prognosis of patients with rheumatoid arthritis (RA) and hepatitis B virus (HBV) surface antigen negative/core antibody positive serostatus (HBsAg-/HBcAb+), who underwent rituximab therapy and developed HBV reactivation. Medical records of RA patients with different HBV serostatus who received rituximab from January 2000 through January 2015 were reviewed. Case notes of four HBsAg-/HBcAb+ patients with RA who had HBV reactivation during treatment with rituximab were excerpted and summarised. We also searched the Medline (PubMed) database to identify published reports of other HBsAg-/HBcAb+ RA patients who likewise developed HBV reactivation during rituximab treatment. The study cohort comprised 54 RA patients who received rituximab, of whom 44 (81.5%) were HBsAg-/HBcAb+ whilst receiving rituximab. Four HBsAg-/HBcAb+ patients had HBV reactivations during rituximab therapy; thus, the incidence of HBV reactivation in the HBsAg-/HBcAb+ group was 9.1%. The literature search discovered another three cases, making a total of at least seven known rituximab-treated HBsAg-/HBcAb+ RA patients who have developed HBV reactivation. The mean duration from the first rituximab infusion to HBV reactivation was 25.4±4.6 months; no fatalities occurred. Approximately 9% of Taiwanese RA patients with HBsAg-/HBcAb+ serostatus had HBV reactivation around 2 years after starting regular rituximab therapy; they all had a relatively good prognosis.

  8. HBS-1: A Modular Child-Size 3D Printed Humanoid

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    Lianjun Wu

    2016-01-01

    Full Text Available An affordable, highly articulated, child-size humanoid robot could potentially be used for various purposes, widening the design space of humanoids for further study. Several findings indicated that normal children and children with autism interact well with humanoids. This paper presents a child-sized humanoid robot (HBS-1 intended primarily for children’s education and rehabilitation. The design approach is based on the design for manufacturing (DFM and the design for assembly (DFA philosophies to realize the robot fully using additive manufacturing. Most parts of the robot are fabricated with acrylonitrile butadiene styrene (ABS using rapid prototyping technology. Servomotors and shape memory alloy actuators are used as actuating mechanisms. The mechanical design, analysis and characterization of the robot are presented in both theoretical and experimental frameworks.

  9. Biochemical and biophysical characterization of maize-derived HBsAg for the development of an oral vaccine.

    Science.gov (United States)

    Shah, Shweta; Hayden, Celine A; Fischer, Maria E; Rao, A Gururaj; Howard, John A

    2015-12-15

    Although a vaccine against hepatitis B virus (HBV) has been available since 1982, it is estimated that 600,000 people die every year due to HBV. An affordable oral vaccine could help alleviate the disease burden and to this end the hepatitis B surface antigen (HBsAg) was expressed in maize. Orally delivered maize material induced the strongest immune response in mice when lipid was extracted by CO2 supercritical fluid extraction (SFE), compared to full fat and hexane-extracted material. The present study provides a biochemical and biophysical basis for these immunological differences by comparing the active ingredient in the differently treated maize material. Purified maize-derived HBsAg underwent biophysical characterization by gel filtration, transmission electron microscopy (TEM), dynamic light scattering (DLS), UV-CD, and fluorescence. Gel filtration showed that HBsAg forms higher-order oligomers and TEM demonstrated virus-like particle (VLP) formation. The VLPs obtained from SFE were more regular in shape and size compared to hexane or full fat material. In addition, SFE-derived HBsAg showed the greatest extent of α-helical structure by far UV-CD spectrum. Fluorescence experiments also revealed differences in protein conformation. This work establishes SFE-treated maize material as a viable oral vaccine candidate and advances the development of the first oral subunit vaccine. Copyright © 2015 Elsevier Inc. All rights reserved.

  10. CO partial pressure dependence of the kinetics of melting of HbS aggregates studied in high concentration phosphate buffer

    Science.gov (United States)

    Aroutiounian, Svetlana

    2002-10-01

    Deoxygenated sickle cell hemoglobin (HbS) monomers enter the polymer phase either by incorporation into a critical nucleus, through heterogeneous nucleation and or through linear growth of the polymers when the concentration of monomers exceeds the solubility. CO-bound, R-state HbS monomers do not polymerize. Thus, polymer melting is enhanced by binding of carbon monoxide (CO) to HbS polymerized monomers. In our study, the melting of HbS aggregates mediated by dilution and CO binding to polymerized monomers is observed with time-resolved extinction spectroscopy. The CO partial pressure (pCO) dependence of the kinetics of melting is studied for pCO = 0, 0.25, 0.5, 0.75, 1 atm with difference progress curves. A phenomenological description with slow and fast relaxation modes reveals a variable relaxation time near the pCO=0.5 due to competition of kinetic mechanisms. The slow component increases with increasing pCO. It has a positive intercept due to the combined action of dilution of the sample and CO-ligation. The pCO dependence is near linear due to non-cooperative CO binding. Significant slowing down of aged samples, most likely due to gelation, is observed. As possible mechanism for variable relaxation time near pCO=0.5atm the fractional percolation threshold is discussed. This work was supported by NIH grant HL58091 (awarded to Daniel. B. Kim-Shapiro).

  11. Survey of HBsAg-positive pregnant women and their infants regarding measures to prevent maternal-infantile transmission

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    Meina Hu

    2010-02-01

    Full Text Available Abstract Background Intrauterine infection is the main contributor to maternal-infantile transmission of HBV. This is a retrospective study of 158 HBsAg-positive pregnant women who delivered children from Jan 1st, 2004 to Dec.31th, 2006 in Wuhan City, China. We investigated the measures taken to prevent maternal-infantile transmission of hepatitis B virus and the infection status of children. Methods HBsAg-positive pregnant women were selected by a random sampling method when they accepted prenatal care in district-level Maternal and Child Health Hospitals. On a voluntary basis, these women completed questionnaires by face-to-face or phone interviews. The collected data were used to evaluate the immunization programs that pregnant women had received for preventing hepatitis B maternal-infantile transmission. Results Among the 158 women, 143(90.5% received Hepatitis B immune globulin during pregnancy, and 86.0% of their children were given Hepatitis B immune globulin and Hepatitis B vaccine. The rate of cesarean section was 82.3%, and 28.5% of these were aimed at preventing HBV infection. The rate of bottle feeding was 51.9%, and 89.0% of bottle feeding cases were for the purpose of preventing HBV infection. There were 71 cases of participants who were HBeAg-positive. Compared with the HBsAg+ HBeAg- group (only HBsAg-positive, the HBsAg + HBeAg+ group (HBsAg-positive and HBeAg-positive had significantly higher rates of the caesarean section and bottle feeding resulting from hepatitis B (P Conclusion Most HBsAg positive pregnant women have a growing awareness of maternal-infantile transmission of Hepatitis B virus and are receiving some form of preventative treatment, like combined immunization. Caesarean and bottle feeding are very common, often primarily to prevent transmission. Relatively few intrauterine infections were identified in this sample, but many infants did not appear to seroconvert after vaccination.

  12. Value of HBsAg level in dynamic monitoring of disease progression in patients with chronic HBV infection

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    BAO Teng

    2017-08-01

    Full Text Available ObjectiveTo investigate the clinical value of HBsAg level in dynamic monitoring of disease progression in patients with chronic HBV infection. MethodsA retrospective analysis was performed for the clinical data of 1107 patients with different clinical stages of chronic HBV infection who had not received antiviral therapy at the time of hospitalization in The Second Affiliated Hospital of Anhui Medical University from May 2011 to December 2015, and according to the disease status, they were divided into HBeAg-positive chronic hepatitis B (CHB group, HBeAg-negative CHB group, compensated liver cirrhosis group (LC-C group, decompensated liver cirrhosis group (LC-D group, and primary liver cancer (PLC group. These groups were compared in terms of HBsAg expression and the association between HBsAg and clinical features. An analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between any two groups; the t-test was used for comparison of continuous data between two groups. The chi-square test was used for comparison of categorical data between these groups. Pearson correlation analysis was also performed. ResultsThere was a significant difference in serum HBsAg level between the HBeAg-positive CHB group, HBeAg-negative CHB group, LC-C group, LC-D group, and PLC group (F=100.45, P<0.001. The HBeAg-positive CHB group had significantly higher levels of HBsAg and HBV DNA than the HBeAg-negative CHB group (t= 16.67 an 16.22, both P<0.001. There were significant differences in HBsAg and HBV DNA levels between the HBeAg-positive CHB group, LC-C group, LC-D group, and PLC group (F= 42.92 and 27.38, both P<0.001, as well as between the HBeAg-negative CHB group, LC-C group, LC-D group, and PLC group (F=6.04 and 4.10, both P<0.05. HBV DNA level was significantly different across patients with different HBsAg levels (<1000 IU/ml, 1000-20 000 IU

  13. Prevalence of Serologic Hepatitis B Markers in Blood Donors From Puebla, Mexico: The Association of Relatively High Levels of Anti-Core Antibodies With the Detection of Surface Antigen and Genomic DNA.

    Science.gov (United States)

    Sosa-Jurado, Francisca; Hilda Rosas-Murrieta, Nora; Guzman-Flores, Belinda; Perez Zempoaltecalt, Cintia; Patricia Sanchez Torres, Ana; Ramirez Rosete, Leticia; Bernal-Soto, Maribel; Marquez-Dominguez, Luis; Melendez-Mena, Daniel; Angel Mendoza Torres, Miguel; Teresa Lopez Delgado, Maria; Reyes-Leyva, Julio; Vallejo-Ruiz, Veronica; Santos-Lopez, Gerardo

    2016-06-01

    The hepatitis B virus (HBV) causes chronic hepatitis, hepatic cirrhosis, and hepatocellular carcinoma. Surface antigen (HBsAg) detection is a definitive test that can confirm HBV infection, while the presence of antibodies against the core protein (anti-HBc) suggests either a previous or ongoing infection or occult hepatitis B infection (OBI). The aim of the present study was to determine the prevalence of anti-HBc and HBsAg in blood donors. Further, the study aimed to estimate the anti-HBc level at which HBV DNA is detected in putative OBI cases, as well as to search for mutations in the "a" determinant associated with the non-detection of HBsAg in serum. We conducted a cross-sectional study from 2003-2009. The study included 120,552 blood donors from the state of Puebla, Mexico. Different commercial systems based on microparticles (enzymatic (MEIA) or chemiluminescent (CMIA)) were used to determine the HBsAg and anti-HBc levels. For the detection of HBV DNA, a nested polymerase chain reaction (nested PCR) was used and the genotypes were determined using Sanger sequencing. Of the 120,552 blood donors, 1437 (1.19%, 95% CI: 1.12 - 1.26) were reactive to anti-HBc, while 82 (0.066%, 95% CI: 0.053 - 0.079) were reactive to HBsAg. Some 156 plasma samples collected in 2009 from anti-HBc-positive/HBsAg-negative blood donors were submitted for HBV DNA detection in a search for probable OBI. Viral DNA was detected in 27/156 (17.3%, 95% CI: 11.5 - 23.1). Our results show an association between HBV DNA or HBsAg and anti-HBc S/CO levels ≥ 4.0. All DNA samples were identified as genotype H and some "a" determinant mutations were identified, although none corresponded to mutations previously reported to hinder the detection of HBsAg by commercial immunoassays. We observed that as the anti-HBc levels increase, there is a higher prevalence of the viral protein HBsAg in blood donors. Samples testing positive for HBV-DNA were seen to exhibit a ten-fold higher presence of anti

  14. Prevalence of Serologic Hepatitis B Markers in Blood Donors From Puebla, Mexico: The Association of Relatively High Levels of Anti-Core Antibodies With the Detection of Surface Antigen and Genomic DNA

    Science.gov (United States)

    Sosa-Jurado, Francisca; Hilda Rosas-Murrieta, Nora; Guzman-Flores, Belinda; Perez Zempoaltecalt, Cintia; Patricia Sanchez Torres, Ana; Ramirez Rosete, Leticia; Bernal-Soto, Maribel; Marquez-Dominguez, Luis; Melendez-Mena, Daniel; Angel Mendoza Torres, Miguel; Teresa Lopez Delgado, Maria; Reyes-Leyva, Julio; Vallejo-Ruiz, Veronica; Santos-Lopez, Gerardo

    2016-01-01

    Background The hepatitis B virus (HBV) causes chronic hepatitis, hepatic cirrhosis, and hepatocellular carcinoma. Surface antigen (HBsAg) detection is a definitive test that can confirm HBV infection, while the presence of antibodies against the core protein (anti-HBc) suggests either a previous or ongoing infection or occult hepatitis B infection (OBI). Objectives The aim of the present study was to determine the prevalence of anti-HBc and HBsAg in blood donors. Further, the study aimed to estimate the anti-HBc level at which HBV DNA is detected in putative OBI cases, as well as to search for mutations in the “a” determinant associated with the non-detection of HBsAg in serum. Patients and Methods We conducted a cross-sectional study from 2003–2009. The study included 120,552 blood donors from the state of Puebla, Mexico. Different commercial systems based on microparticles (enzymatic (MEIA) or chemiluminescent (CMIA)) were used to determine the HBsAg and anti-HBc levels. For the detection of HBV DNA, a nested polymerase chain reaction (nested PCR) was used and the genotypes were determined using Sanger sequencing. Results Of the 120,552 blood donors, 1437 (1.19%, 95% CI: 1.12 - 1.26) were reactive to anti-HBc, while 82 (0.066%, 95% CI: 0.053 - 0.079) were reactive to HBsAg. Some 156 plasma samples collected in 2009 from anti-HBc-positive/HBsAg-negative blood donors were submitted for HBV DNA detection in a search for probable OBI. Viral DNA was detected in 27/156 (17.3%, 95% CI: 11.5 - 23.1). Our results show an association between HBV DNA or HBsAg and anti-HBc S/CO levels ≥ 4.0. All DNA samples were identified as genotype H and some “a” determinant mutations were identified, although none corresponded to mutations previously reported to hinder the detection of HBsAg by commercial immunoassays. Conclusions We observed that as the anti-HBc levels increase, there is a higher prevalence of the viral protein HBsAg in blood donors. Samples testing positive

  15. Detección de anticuerpos circulantes en donantes de sangre en México Detection of antibodies present in blood donors in Mexico

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    José J. Serrano Machuca

    2009-10-01

    Full Text Available OBJETIVO: Detectar anticuerpos circulantes contra seis infecciones transmisibles por sangre en donantes de una institución de seguridad social en Querétaro, México. MÉTODO: Se realizó un estudio transversal descriptivo retrospectivo con datos del Banco de Sangre del Hospital General Regional No. 1, del Instituto Mexicano del Seguro Social (IMSS. Se registraron 6 929 donantes, entre los cuales se identificó a los diagnosticados con cualquier anticuerpo circulante contra brucelosis, enfermedad de Chagas, hepatitis B, hepatitis C, sífilis y VIH. Los casos identificados con cualquiera de estas infecciones se analizaron según edad, género, estado civil, escolaridad, lugar de residencia y número de donaciones. Para estimar la prevalencia total se dividió el número de personas identificadas con cualquiera de los anticuerpos circulantes por el total de donantes, y luego por cada una de las diferentes infecciones. RESULTADOS: De los 6 929 donantes, 144 fueron detectados con algún tipo de anticuerpo circulante de las seis infecciones potencialmente transmisibles por sangre, lo cual da una prevalencia total de 2,07% (0-4,4. Las prevalencias más altas por tipo de anticuerpo circulante correspondieron a la hepatitis C, con 0,721% (IC 95%, 0,522-0,920, y a la enfermedad de Chagas, con 0,649% (IC 95%, 0,460-0,838. CONCLUSIÓN: La identificación de la prevalencia de donantes de sangre con anticuerpos circulantes de alguna de estas seis infecciones potencialmente transmisibles por vía sanguínea permite establecer un perfil epidemiológico propio del banco de sangre del Hospital General Regional No. 1. La enfermedad de Chagas se presenta como emergente, dando pauta a dirigir los esfuerzos para su control.OBJECTIVE: To detect antibodies to six potentially blood-borne infections in blood donors at a social security institute in Querétaro, Mexico. METHODS: A cross-sectional, retrospective, descriptive study was performed using data from the blood

  16. DETECCIÓN DE ANTICUERPOS IgM A LEPTOSPIRA EN HUMANOS EN RIESGO OCUPACIONAL EN VILLAVICENCIO, META

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    Luz Díaz P

    2008-04-01

    Full Text Available Objetivo. Determinar la seroprevalencia a la infección por leptospira en una población humana y los factores de riesgo ocupacional asociados en Villavicencio, Meta. Materiales y métodos. Se aplicó un modelo epidemiológico transversal con selección por conveniencia de grupos e individuos dentro de grupos. Se obtuvieron muestras de sangre por punción cubital de 273 personas correspondientes a 8 grupos de riesgo. Se obtuvo información de factores de riesgo mediante entrevista. Se determinaron anticuerpos IgM mediante ELISA indirecta. Se utilizó una prueba de c2 mediante el programa SPSS 11.0 para Windows. Resultados. La seroprevalencia general fue 19%. Por grupos fue para trabajadores de matadero 7%, veterinarios y auxiliares de clínica de pequeños animales 17%, estudiantes de último año de MVZ 17%, ordeñadores 21%, trabajadores de arrozales 23%, trabajadores de granjas porcícolas 35%, trabajadores de piscícolas 48%. Se encontraron 3 factores asociados, el estrato rural p = 0.0005 ICCR 1.50 a 3.83, tenencia de mascota canina p = 0.046 ICCR 1.03 a 3.26 y contacto con roedores en el trabajo p = 0.000037 ICCR = 1.73 a 4.75. Conclusiones. Se encontró una alta seroprevalencia general de infectados por Leptospira, siendo los grupos de más alta prevalencia los trabajadores piscícolas y de granjas porcícolas. Se reconoce una vez más el carácter ocupacional de la infección posiblemente por falta de medidas de higiene y protección laboral.

  17. OBTENCIÓN DE ANTICUERPOS ESPECÍFICOS PARA LA DETECCIÓN DEL VIRUS TaLMV DEL TOMATE DE ÁRBOL

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    Yuliana Marcela Gallo García

    2011-05-01

    Full Text Available En Colombia el rendimiento del cultivo de árbol se ha visto seriamente afectado por la expansión de la enfermedad conocida como virosis. Esta patología se registró inicialmente en 1991 en el norte de Antioquia y su expansión ha alcanzado todas las regiones cultivadoras de este frutal en el país. Trabajos recientes han establecido la presencia de al menos dos especies de potyvirus asociadas a la virosis en los cultivos de tomate de árbol de Antioquia: Potato virus Y (PVY y Tamarillo leaf malformation virus (TaLMV, especie propuesta. Con el fin de reducir la diseminación de estos patógenos virales en el país, es necesario contar con herramientas de diagnóstico que permitan la certificación del material de siembra y la detección temprana en plantas asintomáticas. En este trabajo se presenta la obtención de anticuerpos específicos para la detección del virus TaLMV utilizando una región antigénica de 15 residuos de la cápside viral. La sensibilidad y especificidad de los anticuerpos fue evaluada mediante pruebas de ELISA y dot-blot utilizando proteína recombinante y péptidos sintéticos. La utilidad de estos anticuerpos fue validada a partir de una prueba piloto de detección de TaLMV en muestras de plantas de tomate de árbol obtenidas en el Oriente Antioqueño y los resultados comparados con los niveles de detección que ofrece la técnica de RT-PCR con cebadores específicos para la cápside viral de TaLMV.

  18. Anticuerpos antinucleares, imágenes y características obtenidas por inmunofluorescencia: Importancia de los isotipos IgA, IgM e IgG Antinuclear antibodies, patterns and characteristics obtained by immunofluorescence: The importance of the IgA, IgM and IgG isotypes

    Directory of Open Access Journals (Sweden)

    Miriam Arcavi

    2009-10-01

    Full Text Available La técnica de elección para el screening de anticuerpos antinucleares (ANA es la inmunofluorescencia indirecta que utiliza como sustrato una línea de células epiteliales de carcinoma de laringe humano (IFI-HEp2, y como antisuero, anti-IgG o anti-Ig totales. Los ANA-IgG son los más importantes para el diagnóstico y monitoreo de las enfermedades del tejido conectivo (ETC, mientras los ANA-IgM son de menor relevancia clínica en estos pacientes. Sin embargo, poco se sabe de los ANA-IgA ya que estos Ac han sido menos investigados. El objetivo de este trabajo fue estudiar la prevalencia de los diferentes isotipos de inmunoglobulinas de anticuerpos antinucleares en los pacientes con ETC y evaluar la conveniencia de utilizar conjugados monovalentes o polivalentes. Se procesaron 100 sueros de pacientes con diversas ETC empleando IFI-HEp2, en los cuales se detectó 38% de ANA-IgA (títulos ≥ 1:80 y 12% de ANA-IgM (títulos ≤ 1:160. En 29 casos se detectó IgA en ausencia de IgM, en 3 casos IgM en ausencia de IgA. En todos los casos los ANA-IgG estuvieron presentes. En 6 sueros se observó un cambio de imagen con conjugado anti-IgA y en 3 con conjugado anti-IgM. Debido a la alta prevalencia de ANA-IgA detectada por IFI-HEp2, se destaca la conveniencia de utilizar conjugado anti-Ig totales en lugar de anti-IgG, mientras se desconozca la relevancia de los ANA-IgA en el diagnóstico, pronóstico y seguimiento de las enfermedades reumáticas sistémicas.The indirect immunofluorescence with epitelial cell line from human laryngeal carcinoma as substrate (IIF-HEp2 and anti-IgG or anti-total Ig as antisera, is the technique currently used for the detection of antinuclear antibodies. The most important antibodies for the diagnosis and follow-up of connective tissue diseases (CTD are the IgG-ANA, while the IgM-ANA have no clinical relevance. However the IgA-ANA have not been thoroughly investigated so far. The aim of this work was to study the prevalence

  19. Factors Predicting HBsAg Seroclearance and Alanine Transaminase Elevation in HBeAg-Negative Hepatitis B Virus-Infected Patients with Persistently Normal Liver Function.

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    Tai-Long Chien

    Full Text Available A certain proportion of hepatitis B virus (HBV-infected patients with persistently normal alanine transaminase (ALT levels have significant fibrosis. Using liver stiffness measurements (Fibroscan® and laboratory data, including serum ALT, quantitative HBsAg (qHBsAg, and HBV DNA, we attempted to predict the natural histories of these patients.Non-cirrhotic HBeAg-negative chronic hepatitis B patients with persistently normal ALT were followed up prospectively with the end points of HBsAg seroclearance and ALT elevation above the upper limit of normal. The factors that were predictive of the end points were identified.A total of 235 patients with an average age of 48.1 +/- 10.7 years were followed up for 7 years. Eight patients (3.4% lost HBsAg, and 15 patients (6.4% experienced ALT elevation. The overall cumulative HBsAg seroclearances were 0.4%, 1.3% and 2.3% at years 1, 3 and 5, respectively. Regarding HBsAg seroclearance, the qHBsAg (< 30 IU/ml cutoff resulted in a hazard ratio (HR of 19.6 with a 95% confidence interval (CI of 2.2-166.7 (P = 0.008. The baseline ALT level (odd ratio (OR 1.075, 95% CI 1.020-1.132, P = 0.006 and a qHBsAg above 1000 IU/ml (3.7, 1.1-12.4, P = 0.032 were associated with ALT elevation. Limited to men, the baseline liver stiffness (1.6, 1.0-2.5, P = 0.031 and a qHBsAg above 1000 IU/ml (10.4, 2.1-52.4, P = 0.004 were factors that were independently associated with ALT elevation.A low qHBsAg level predicted HBsAg clearance. Baseline ALT and a qHBsAg above 1000 IU/ml were independent predictive factors for ALT elevation. Among the men, the independent predictive factors for ALT elevation were qHBsAg and liver stiffness.

  20. ALT flares during nucleotide analogue therapy are associated with HBsAg loss in genotype A HBeAg-positive chronic hepatitis B.

    Science.gov (United States)

    Wong, Darren; Littlejohn, Margaret; Edwards, Rosalind; Jackson, Kathy; Revill, Peter; Gaggar, Anuj; Kitrinos, Kathryn; Subramanian, Mani; Marcellin, Patrick; Buti-Ferret, Maria; Janssen, Harry; Gane, Ed; Locarnini, Stephen; Thompson, Alexander

    2018-02-10

    Alanine aminotransferase (ALT) flares during NA therapy are uncommon but occur. Evaluation of ALT flares during nucleos(t)ide analogue (NA) therapy is important as new immunomodulatory therapies for hepatitis B virus (HBV) are developed. We evaluated the association between ALT flares and HBsAg loss during long-term therapy for genotype A CHB. This analysis included genotype A subjects from a phase III study of tenofovir vs adefovir in HBeAg-positive HBV. ALT flare was defined as (i) a rise in ALT >2x ULN from normal ALT levels; or (ii) a rise in ALT >2x baseline ALT level. HBsAg response at week 384 was recorded as one of HBsAg loss vs HBsAg decline (≥1 log 10  IU/mL decline) vs non-response. The primary analysis evaluated the association between ALT flare and HBsAg response. 54 subjects were included. 23/54 (43%) subjects experienced an on-treatment ALT flare. 45% achieved an HBsAg reduction ≥1 log 10  IU/mL, and of these 67% achieved HBsAg loss at a median of 102 weeks [IQR: 64-156]. Flare was associated with HBsAg decline vs non-response (67% vs 23%, P = .002), and were more common in subjects who achieved HBsAg loss vs non-response (56% vs 23%), P = .049). There was a median delay of 56 weeks [IQR: 40-80] between a flare and HBsAg loss. In genotype A subjects undergoing long-term NA therapy, ALT flares predict for HBsAg response. The delay between ALT flare and HBsAg loss has implications for clinical trial design for early phase development of immunomodulatory strategies aiming for HBsAg loss. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  1. Activación, producción de anticuerpos y apoptosis del linfocito b en la inmunodeficiencia variable común

    OpenAIRE

    Clemente Ximenis, Antonio

    2014-01-01

    La Inmunodeficiencia Variable Común es un síndrome heterogéneo caracterizado por hipogammaglobulinemia e infecciones recurrentes. La causa no se conoce, aunque se ha propuesto un fallo en la diferenciación final del linfocito B hacia célula de memoria o célula plasmática productora de anticuerpos. Los pacientes pueden clasificarse en 3 grupos (MB0, MB1 o MB2), con distinta presentación y evolución clínica, en función del grado de alteración de los linfocitos B de memoria. Estudiamos si distin...

  2. Determinación de anticuerpos contra Neospora caninum en búfalos de agua (Bubalus bubalis) en la Amazonía peruana

    OpenAIRE

    Jara V., Javier; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Chávez V., Amanda; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Casas A., Eva; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Sánchez P., Nofre; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Moreno-López, Jorge; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Merza, Malik; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

    2011-01-01

    La neosporosis es una parasitosis de importancia en el sector ganadero por ser causante de abortos y mortalidad neonatal. El objetivo del estudio fue determinar la presencia de anticuerpos contra Neospora caninum en búfalos de agua procedentes de cinco centros de crianza ubicados en el distrito Jenaro Herrera, departamento de Loreto, Perú, en el año 2008. Se evaluaron 83 muestras de suero, obtenidas de búfalas en edad productiva, criadas para la producción de leche. Las muestras se evaluaron ...

  3. Anticuerpos antiprotrombina y resistencia adquirida a la proteína C activada en el síndrome antifosfolipídico

    OpenAIRE

    Muñoz Rodríguez, Francisco José

    2003-01-01

    El síndrome antifosfolipídico (SAF) se caracteriza clínicamente por la aparición de fenómenos trombóticos recurrentes, complicaciones obstétricas, tales como abortos o pérdidas fetales recurrentes, y trombocitopenia. Inmunológicamente se define por la presencia de los anticuerpos antifosfolipídicos (AAF), un grupo heterogéneo de autoanticuerpos que reconocen complejos constituidos por fosfolípidos de membrana y proteínas plasmáticas que actúan como cofactores. Los mejor conocidos y que ayudan...

  4. Frecuencia de anticuerpos contra el virus C de la hepatitis en pacientes con cirrosis hepática en Yucatán, México

    OpenAIRE

    Góngora-Biachi Renán A; Castro-Sansores Carlos J; González-Martínez Pedro; Lara-Perera Dora M; Garrido-Palma Julio; Lara-Perera Víctor

    2003-01-01

    OBJETIVO: En este estudio reportamos la prevalencia de anticuerpos contra el virus C de la hepatitis (Ac-VCH) en un grupo de pacientes con cirrosis hepática (CH). MATERIAL Y MÉTODOS: Se hizo un estudio prospectivo, transversal y descriptivo, de marzo de 1998 a mayo de 1999. Se estudiaron a 153 pacientes (117 (76%) hombres y 36 (24%) mujeres) con diagnóstico de CH, que eran atendidos en el Hospital General Agustín O' Horan y en el Centro de Investigaciones Regionales Doctor Hideyo Noguchi, en ...

  5. Desarrollo de una técnica de inmunoelectrotransferencia "Westernblot" para la detección de anticuerpos contra componentes proteínicos de Salmonella Typhi

    Directory of Open Access Journals (Sweden)

    Miguel Guzmán Urrego

    1990-12-01

    Full Text Available Desde su desarrollo la lnmunoelectrotransferencia (INMET ha sido una herramienta útil en biología molecular así como también en el diagnóstico de diferentes enfermedades infecciosas. Tradicionalniente la demostración de anticuerpos contra Salmonella typhi, se ha enfocado hacia la detección de aquellos, que se producen contra lipopolisacáridos, especialmente los que se relacionan con el antígeno somático "0". Pero pocos trabajos, se han realizado para la detección de anticuerpos contra otros antígenos tales como proteínas protoplasmáticas. Este trabajo intenta demostrar si tales anticuerpos se producen en conejos inmunizados experimentalmente, así como también en voluntarios vacunados contra la fiebre Tifoidea con lavacuna oral (cepa Ty21a. El antígeno preparado y purificado a partir de una cepa de Salmonella typhi, fue suspendido en una solución tampón y sonicado para obtener sus productos celulares; de esta manera y con la adecuada concentración de proteínas fue separado en gel de poliacrilamida en sus diferentes fracciones antigénicas y luego transferidos a membranas de nitrocelulosa en donde por medio de reacción inmunoenzimática, se visualizó la banda característica, al poner en contacto la membrana con el antisuero hiperinmune obtenido en conejos (Modelo Experimental y el suero de voluntarios antes y después de la inmunización (Modelo  Humano. La banda visualizada correspondió a un peso aproximado de 38.000 kd. Trece de los voluntarios (32.5%, presentaron esta misma banda antes de la inmunización, la cual, aunque más tenue, representó un complejo antígeno-anticuerpo, lo cual, induce a pensar en un posible contacto con el microorganismo en el pasado. La posibilidad de la reacción cruzada se descartó realizando el mismo procedimiento con lisados de Escherichia coli.

  6. The hybrid EIA test: a specific and sensitive assay for the detection of woodchuck antibody to hepatitis surface antigen (anti-WHs).

    Science.gov (United States)

    Millman, I; Glass, R G

    1988-05-01

    'Ausria II' polystyrene beads (Abbott Labs, N. Chicago) are reacted with woodchuck serum positive for WHsAg in a dilution predetermined by titration. This modified bead is used in a blocking assay to detect the presence of antibody to the surface antigen of woodchuck hepatitis virus (anti-WHs). Serum containing woodchuck anti-WHs and commercial horseradish peroxidase (HRP) labeled anti-HBs are sequentially added. A drop in optical density at 492 nm of 50% or more due to the blocking of HRP conjugated anti-HBs by anti-WHs compared with a control (negative woodchuck serum) is a measure of anti-WHs. The ease and simplicity of converting readily available 'Ausria II' beads to specific reagents for detecting anti-WHs should be welcomed by investigators studying WHV. The method described is both sensitive and reproducible.

  7. Caracterización del antígeno reconocido por el anticuerpo monoclonal M22.8 y su posible implicación en la biomineralización de bivalvos

    OpenAIRE

    Calvo Iglesias, Juan

    2017-01-01

    En el laboratorio de Inmunología de la Universidad de Vigo se obtuvieron anticuerpos monoclonales dirigidos específicamente frente a larvas de mejillón. Desde su obtención, se han optimizado los métodos de detección específica de dichas larvas, y se vienen empleando de forma rutinaria por parte de la Fundación Cetmar desde finales de 2008 en el muestreo semanal que se realiza para conocer la distribución espacio-temporal de larvas. Uno de los anticuerpos, el M22.8 se ha demostrado que...

  8. Development of an economic and efficient strategy to detect HBsAg: application of "gray-zones" in ELISA and combined use of several detection assays.

    Science.gov (United States)

    Peng, Jing; Cheng, Liming; Yin, Botao; Guan, Qing; Liu, Yanling; Wu, Shiji; Wang, Bin; Tang, Ning; Zhang, Biyu; Wang, Lian; Yang, Dongliang; Sun, Ziyong

    2011-11-20

    ELISA and CMIA are commonly used for detection of HBsAg. However, few investigations have been performed to evaluate their value in clinical practice, especially when jointly used. A reasonable and economic HBsAg testing algorithm is in great need. A total of 161,426 specimens in China were tested for 5 serum HBV markers with commonly used ELISA kits. 498 of these specimens were further tested for HBsAg by another ELISA kit, a CMIA kit and an HBsAg confirmatory assay. The sensitivities of the 2 ELISA kits were 76.21% and 88.42%, respectively. However, when using "gray-zones", the sensitivities were significantly improved to 97.43% and 96.43%. Furthermore, the combined use of the 2 ELISA kits and their "gray-zones" improved the sensitivity to 99.04%. Nevertheless, 2.91% of the samples with S/CO values below the lower "gray-zone" limits were reactive by the CMIA kit and then confirmed as HBsAg positive. However, 71.43% of the samples with HBsAg values within 0.05 and 0.10 IU/ml detected by the CMIA kit could not be confirmed. As a rational and economic strategy, combined use of "gray-zones" in ELISA and several different detection assays can significantly increase the efficiency of HBsAg detection. Copyright © 2011 Elsevier B.V. All rights reserved.

  9. [Predictive study of HBsAg in different stages of neonatal venous blood on failure of blocking HBV mother to infant transmission].

    Science.gov (United States)

    Yi, Wei; Li, Ming-Hui; Hu, Yu-Hong; Liu, Feng; Zhang, Yang-Li; Liu, Xue-Jing; Hao, Hong-Xiao; Song, Shu-Jing; Liu, Ying; Li, Xing-Hong; Sun, Ji-Yun; Liu, Min; Cheng, Jun; Xie, Yao

    2011-10-01

    In this study, we discuss the predictive value of different content of HBsAg in different stages of neotal venous blood on failure of blocking mother to infant transmission of HBV. 150 infants born of chronically HBV infected mothers who were positive of both HBsAg and HBeAg and who also had a HBV DNA virus load above 10(5) copies/ml were enrolled. These infants were given hepatitis B virus immune globin (HBIG) 200 IU immediately after birth and were given hepatitis B vaccine 10 or 20 microg at brith, 1 month and 6 months after birth. HBV serological index of these infants were test at birth, 1 month and 7 months after birth respectively. Different content of HBsAg in different stages of neonatal venus blood were analyzed to predict the failure of blocking mother to infant transmission of HBV. 11 infants failed in blocking of HBV mother to infant transmission. The positive rate of HBsAg at birth, 1 month and 7 months after birth were 41.26%, 10.49% and 7.69% respectively, and were 97.90%, 65.73% and 13.29% of HBeAg. The positive predictive value of HBsAg > or = 0.05 and HBsAg > or = 1 IU/ml at birth were 18.64% and 70% respectively, and were 73.33% and 100% one month after birth. Infants with HBsAg > or = 1 IU/ml at birth should be suspicious of failure on blocking HBV mother-to-infant transmission and it should be more credible if the infant has HBsAg > or = 1 IU/ml one month after birth. How to improve the blocking rate of neonates who were positive of HBsAg at birth and one month after birth should be the focus of our future research.

  10. Predictive role of serum HBsAg and HBcrAg kinetics in patients with HBeAg-negative chronic hepatitis B receiving pegylated interferon-based therapy.

    Science.gov (United States)

    Chuaypen, N; Posuwan, N; Chittmittraprap, S; Hirankarn, N; Treeprasertsuk, S; Tanaka, Y; Shinkai, N; Poovorawan, Y; Tangkijvanich, P

    2018-03-01

    To investigate the role of serum hepatitis B core-related antigen (HBcrAg) kinetics in predicting long-term outcome of pegylated interferon (PEG-IFN)-based therapy in patients with hepatitis B e antigen (HBeAg)-negative chronic hepatitis B (CHB). A total of 121 Thai patients with HBeAg-negative CHB recruited from a previous randomized trial of 48-week PEG-IFN alone or combined with entecavir were enrolled. Hepatitis B surface antigen (HBsAg) and HBcrAg levels were serially examined. Paired biopsy samples taken at baseline and after treatment were assessed for intrahepatic covalently closed circular DNA (cccDNA). Persistent virologic remission (PVR, defined by persistent hepatitis B virus (HBV) DNA <2000 IU/mL) and HBsAg clearance at 3 years after treatment were 29% (35/121) and 9% (11/121) respectively. Baseline HBcrAg correlated with HBV DNA and cccDNA but not with HBsAg. Baseline HBsAg, as well as HBsAg and HBcrAg, declines were associated with PVR, while HBsAg decline was predictive of HBsAg clearance. High baseline antigen levels (HBsAg ≥3.4 log 10 IU/mL plus HBcrAg ≥3.7 log 10 U/mL) yielded high negative predictive values of PVR (45/50, 90%) and HBsAg clearance (50/50, 100%). At week 12, declines of HBsAg, HBcrAg and both antigens combined of <0.5 log 10 yielded negative predictive values for PVR of 90% (71/79), 82% (61/74) and 96% (48/50) respectively. Quantitative HBcrAg was significantly associated with cccDNA in HBeAg-negative CHB. This novel antigen, together with HBsAg, could identify patients with low probability of PVR and HBsAg clearance in long-term follow-up. Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  11. Seven-Year Treatment Outcome of Entecavir in a Real-World Cohort: Effects on Clinical Parameters, HBsAg and HBcrAg Levels.

    Science.gov (United States)

    Lam, Yuk-Fai; Seto, Wai-Kay; Wong, Danny; Cheung, Ka-Shing; Fung, James; Mak, Lung-Yi; Yuen, John; Chong, Chun-Kong; Lai, Ching-Lung; Yuen, Man-Fung

    2017-10-26

    We aimed to determine the levels of alanine aminotransferase (ALT), hepatitis B virus DNA (HBV DNA), HBsAg, and a novel viral marker (hepatitis B core-related antigen (HBcrAg)); hepatitis B e antigen (HBeAg) seroconversion and drug resistance rates after 7 years of entecavir treatment in chronic hepatitis B (CHB) patients. Two hundred and twenty-two Chinese CHB patients on continuous entecavir treatment were recruited. Serologic, virologic, biochemical outcomes, and the occurrence of entecavir signature mutations were determined. The rates of ALT normalization, HBeAg seroconversion, and undetectable HBV DNA were 98.3%, 82.1%, and 98.7%, respectively, after 7 years of entecavir treatment. The genotypic resistance rate was 1.2%. Decline of HBsAg level was modest with a median decline rate of 0.107 log IU/ml/year. Among patients with baseline HBsAg <1,000 IU/ml and annual HBsAg decline rate of ≥0.166 log IU/ml, all have HBsAg of <200 IU/ml (a level highly predictive for HBsAg seroclearance) at year 7. In contrast, in patients with baseline HBsAg ≥1,000 IU/ml and annual HBsAg decline rate of <0.166 log IU/ml, 95.5% had HBsAg of ≥200 IU/ml at year 7. Decline of HBcrAg levels was moderate with a median decline rate of 0.244 log kU/ml/year. Forty-seven patients (32.0%) had undetectable HBcrAg level at year 7. Long-term entecavir therapy continued to have good responses with low drug resistance rate. However, the decline of HBsAg with treatment was suboptimal. HBcrAg level declined at a relatively better rate. Baseline HBsAg level of <1,000 IU/ml and annual decline of 0.166 log IU/ml could be used to predict HBsAg response.

  12. Eficacia experimental de anticuerpos IgY producidos en huevos, contra el veneno de la serpiente peruana Bothrops atrox Experimental efficacy of IgY antibodies produced in eggs against the venom of the Peruvian snake Bothrops atrox

    Directory of Open Access Journals (Sweden)

    Julio C. Mendoza

    2012-03-01

    Full Text Available Objetivos. Desarrollar un protocolo de inmunización para producir inmunoglobulinas IgY de origen aviar contra el veneno de la serpiente peruana Bothrops atrox y evaluar la capacidad neutralizante. Materiales y métodos. Se inmunizaron seis gallinas de postura de la raza hy line brown con 500 μg/dosis de veneno de B. atrox en un periodo de dos meses. Cada semana, los huevos fueron colectados para el aislamiento de inmunoglobulinas IgY a partir de la yema, usando dos pasos consecutivos con αcido caprνlico y sulfato de amonio. La detecciσn de anticuerpos se realizσ por inmunodifusiσn doble mientras que el tνtulo y reactividad cruzada se determinaron por las técnicas de ELISA y Western blot. El cálculo de DL50 y de la DE50 del antiveneno IgY producido se realizó utilizando el método de Probits. Resultados. La masa de anticuerpos aislados fue de 8,5 ± 1,35 mg de IgY/mL de yema. Asimismo, la DE50 del antiveneno aviar fue calculada en 575 μL de antiveneno/mg de veneno. Adicionalmente, los ensayos de reactividad cruzada mostraron que el veneno de B. atrox comparte mas epνtopes comunes con el veneno de B. brazili (47% que con otros veneno del mismo género, en tanto que los venenos de Lachesis muta (19% y Crotalus durissus (12% mostraron una baja reactividad cruzada. Conclusiones. Se ha obtenido IgY purificada contra el veneno de B. atrox con capacidad neutralizante y se ha demostrado su utilidad como herramienta inmunoanalítica para evaluar la reactividad cruzada con venenos de otras especies.Objectives. To develop an immunization protocol in order to produce avian IgY immunoglobulins against Bothrops atrox Peruvian snake venom and to evaluate its neutralizing capacity. Materials and methods. Six Hy Line Brown hens were immunized each two weeks using 500μg/doses of B. atrox venom in a period of two months. Each week, eggs were collected for IgY isolation from yolk using two consecutive steps with caprilic acid and ammonium sulfate

  13. Veterans health administration hepatitis B testing and treatment with anti-CD20 antibody administration.

    Science.gov (United States)

    Hunt, Christine M; Beste, Lauren A; Lowy, Elliott; Suzuki, Ayako; Moylan, Cynthia A; Tillmann, Hans L; Ioannou, George N; Lim, Joseph K; Kelley, Michael J; Provenzale, Dawn

    2016-05-21

    To evaluate pretreatment hepatitis B virus (HBV) testing, vaccination, and antiviral treatment rates in Veterans Affairs patients receiving anti-CD20 Ab for quality improvement. We performed a retrospective cohort study using a national repository of Veterans Health Administration (VHA) electronic health record data. We identified all patients receiving anti-CD20 Ab treatment (2002-2014). We ascertained patient demographics, laboratory results, HBV vaccination status (from vaccination records), pharmacy data, and vital status. The high risk period for HBV reactivation is during anti-CD20 Ab treatment and 12 mo follow up. Therefore, we analyzed those who were followed to death or for at least 12 mo after completing anti-CD20 Ab. Pretreatment serologic tests were used to categorize chronic HBV (hepatitis B surface antigen positive or HBsAg+), past HBV (HBsAg-, hepatitis B core antibody positive or HBcAb+), resolved HBV (HBsAg-, HBcAb+, hepatitis B surface antibody positive or HBsAb+), likely prior vaccination (isolated HBsAb+), HBV negative (HBsAg-, HBcAb-), or unknown. Acute hepatitis B was defined by the appearance of HBsAg+ in the high risk period in patients who were pretreatment HBV negative. We assessed HBV antiviral treatment and the incidence of hepatitis, liver failure, and death during the high risk period. Cumulative hepatitis, liver failure, and death after anti-CD20 Ab initiation were compared by HBV disease categories and differences compared using the χ(2) test. Mean time to hepatitis peak alanine aminotransferase, liver failure, and death relative to anti-CD20 Ab administration and follow-up were also compared by HBV disease group. Among 19304 VHA patients who received anti-CD20 Ab, 10224 (53%) had pretreatment HBsAg testing during the study period, with 49% and 43% tested for HBsAg and HBcAb, respectively within 6 mo pretreatment in 2014. Of those tested, 2% (167/10224) had chronic HBV, 4% (326/7903) past HBV, 5% (427/8110) resolved HBV, 8% (628

  14. Obtención y caracterización de un anticuerpo específico contra la hormona luteinizante humana

    Directory of Open Access Journals (Sweden)

    Adriana Umaña

    1996-12-01

    Full Text Available En el presente trabajo se describe la obtención del anticuerpo policlonal específico contra la hormona luteinizante humana (HL. La hormona fue purificada a partir de una fracción rica en glicoproteínas, obtenida de hipófisis humanas, siguiendo el método desarrollado previamente en el proyecto basado en diferencias de solubilidad y constante dieléctrica entre las diferentes proteínas. El antisuero específico se obtuvo por inoculación del antígeno en conejos raza Nueva Zelanda y su producción controlada valorando el título por RIA. La caracterización parcial del antisuero se realizó mediante la determinación del título óptimo, afinidad, especificidad y su aplicabilidad para la cuantificación en términos de curva estándar y control de calidad, en comparación con un antisuero de referencia internacional. Los resultados indicaron que el anticuerpo obtenido puede emplearse en la cuantificación de HL por RIA.

  15. Detección de anticuerpos contra Borrelia burgdorferi e identificación de garrapatas ixodidas en Piura Y Amazonas, Perú

    Directory of Open Access Journals (Sweden)

    Martha Glenny A

    2004-01-01

    Full Text Available Objetivos: Detectar anticuerpos IgG/IgM contra Borrelia burgdorferi en población general, procedentes de los departamentos de Piura y Amazonas e identificar especies de garrapatas probablemente incriminadas en la transmisión de la enfermedad de Lyme. Material y Métodos: Entre agosto del año 2001 y junio de 2002, se colectaron muestras de sangre de 232 pobladores procedentes de ocho localidades del Departamento de Piura y 12 del Departamento de Amazonas, para evaluar mediante ELISA Captia™ Lyme IgG/IgM (Trinity biotech la presencia de anticuerpos contra Borrelia burgdorferi. Además, se colectaron garrapatas en animales domésticos por búsqueda directa. Resultados: Se detectó seropositividad en 9,9 % de los sueros evaluados. Asimismo, de 433 garrapatas colectadas se identificaron los géneros: Ixodes (5,5%, Amblyomma (18,0%, Rhipicephalus (23,5%, Anocentor (31,1% y Boophilus (21,7%. Conclusiones: Existen personas seropositivas por Borrelia en Piura y Amazonas, coincidiendo con los hallazgos realizados en Sapillica en el año 1992, además se detectó la presencia de garrapatas del género Ixodes en Piura.

  16. Helicobacter pylori y anticuerpos antiislotes pancreáticos en la diabetes mellitus Helicobacter pylori and islet cells antibodies in diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Eduardo Cabrera Rode

    2002-04-01

    Full Text Available Se estudiaron 66 sujetos ICA +: 21 diabéticos tipo 1, 24 diabéticos clasificados inicialmente como de tipo 2 y 21 familiares de primer grado de diabéticos tipo 1, se compararon con 101 individuos ICA -: 20 diabéticos tipo 2, 20 FPG, 21 niños y 40 adultos controles no diabéticos para analizar si la respuesta inmunológica a H. pylori está asociada con anticuerpos antiislotes pancreáticos (ICA. Los ICA se determinaron por el método de inmunofluorescencia indirecta con incubación prolongada y los niveles de anticuerpos (IgG contra H. pylori (HP, por un ELISA utilizando un kit comercial. Se observó frecuencia de anticuerpos (IgG contra H. pylori del 38,0 % (25/66 en los sujetos ICA + y del 39,6 % (40/101 en los ICA-. No se halló correlación entre la presencia de anticuerpos (IgG contra H. pylori y los ICA en los grupos estudiados. No hubo diferencias en relación con la presencia de anticuerpos IgG contra H. pylori entre la DM y los controles (47,7 % [31/65] vs. 40,9 % [25/61]. Se encontró alto porcentaje de anticuerpos (IgG contra H. pylori en los diabéticos tipo 1 (33 %, 7/21 en relación con los controles (14 %, 3/21, pero sin diferencias significativas. Existe mayor frecuencia de anticuerpos contra Helicobacter en los controles adultos, en comparación con los niños (55 %, 22/40 vs. 14 %, 3/21, p = 0,0025. La frecuencia de anticuerpos contra H. pylori está mucho más elevada en los controles adultos quizá por la mayor posibilidad de exposición a reinfecciones. A pesar de estos resultados, no excluimos que la infección por H. pylori esté relacionada con la diabetes tipo 1, porque algunos estudios sugieren que la IgG contra H. pylori no persiste por largo tiempo.66 ICA-positive patients were studied: 21 type 1 diabetics, 24 diabetics initially classified as type 2 and 21 first-degree relatives of type 1 diabetics. They were compared with 101 ICA-negative patients: 20 type 2 diabetics, 20 first-degree relatives, 21 children and

  17. Prevalencia de anticuerpos antimaláricos en donantes del banco de sangre del Hospital Universitario San Vicente de Paúl de Medellín, Colombia

    Directory of Open Access Journals (Sweden)

    Jhon M. Ortíz

    1999-12-01

    Full Text Available Se determinó la prevalencia de anticuerpos antimaláricos, tipo IgG, mediante la técnica de inmunofluorescencia indirecta (IFI, en 392 sueros de donantes voluntarios de sangre del Hospital Universitario San Vicente de Paúl, recolectados durante los meses de septiembre y octubre del año 1995 y mantenidos en congelación a -70 ""C durante 36 meses. La prevalencia de seropositividad fue de 1,8% (7 donantes, de los cuales, tres tenían el antecedente de haber vivido en zona endémica para malaria; de éstos, dos habían sufrido malaria, por lo menos, una vez en la vida; los cuatro donantes restantes no habían vivido en zona endémica para malaria ni tenían antecedentes de haberla padecido. Todos los donantes tuvieron gotas gruesas negativas para Plasmodium. Para tratar de reducir el riesgo de malaria transfusional, se recomienda informar en forma clara a los donantes sobre lasáreas endémicas para malaria en el departamento, complementar la encuesta que realizan los bancos de sangre con gota gruesa en todas las unidades de sangre que se vayan a transfundir y determinar anticuerpos antimaláricos en todos los donantes de riesgo con encuestas sospechosas y que puedan ser transmisores potenciales de Plasmodium.

  18. High brilliant thermal and cold moderator for the HBS neutron source project Jülich

    International Nuclear Information System (INIS)

    Cronert, T; Zakalek, P; Rücker, U; Brückel, T; Dabruck, J P; Doege, P E; Nabbi, R; Bessler, Y; Hofmann, M; Butzek, M; Klaus, M; Lange, C; Hansen, W

    2016-01-01

    The proposed High Brilliance Neutron Source (HBS), recognized within the Helmholtz Association of German Research Centres, will optimize the entire chain from particle source through particle accelerator, target, moderator, reflector, shielding, beam extraction, beam transport all the way to the detector, utilizing the nuclear Be(p,n) or Be(d,n) reaction in the lower MeV energy range. A D 2 O moderating reflector prototype (MRP) and a cold source were constructed and build according to MCNP parameter studies. The MRP was tested in a feasibility study at the TREFF instrument at MLZ (Garching). Cold beam extraction from the flux maximum within the moderator based on liquid para H 2 and other cold moderators will be tested by energy spectroscopy via TOF-method. Different ratios of liquid ortho/para H 2 will be fed to the cold moderator. The ratio will be controlled by feeding from reservoires of natural liquid H 2 and a storage loop with an ortho/para converter and determined via online heat capacity measurement. (paper)

  19. Immunomodulatory effects of HBsAg vaccine and levamisole in chronic hepatitis B and hepatitis B carrier children.

    Science.gov (United States)

    Demirci, Fikret; Bayraktaroğlu, Ziya; Karaoğlan, Murat; Coşkun, Yavuz; Karaoğlan, Ilkay; Okan, Vahap

    2005-12-01

    Pathogenesis of chronic hepatitis B and hepatitis B carrier status is related to deficiencies in the immune system. Thus, treatments regulating the immune system are under discussion. The aim of this study was to investigate the effects of HBsAg vaccine and levamisole on lymphocyte subgroups and immunoglobulins in children with chronic hepatitis B and hepatitis B carriers. A total of 93 naive children (43 chronic hepatitis B carriers, 50 chronic hepatitis B patients) were treated in three groups with HBsAg vaccine, levamisole or levamisole plus HBsAg vaccine. Levamisole (ketrax) was delivered as 2.5 mg/kg/day per os, three times per week for three months; the vaccine (Gen HevacB) was administered subcutaneously as 20, 30, 40 microg at one-month intervals. Both medications were delivered at same dosages in the combined group. The examinations were performed at pre-treatment and at the end of the third month when the treatment concluded. After treatments, CD3, CD4 and CD4/CD8 significantly increased and CD8 significantly decreased in chronic hepatitis B patient groups, except in the levamisole treated group. IgG and IgA were significantly decreased in all groups of chronic hepatitis B patients. It was found that HBsAg vaccine induced cellular immunostimulation in children with chronic hepatitis B; however, levamisole did not. The immune cells of hepatitis B carriers did not manifest a significant change in any treatment group. Although there was no change in B-cell, significant decreases were determined in immunoglobulins (IgG, IgA), especially in chronic hepatitis B patients.

  20. A pilot external quality assurance study of transfusion screening for HIV, HCV and HBsAG in 12 African countries.

    Science.gov (United States)

    Bloch, E M; Shah, A; Kaidarova, Z; Laperche, S; Lefrere, J-J; van Hasselt, J; Zacharias, P; Murphy, E L

    2014-11-01

    Serologic screening for the major transfusion transmissible viruses (TTV) is critical to blood safety and has been widely implemented. However, actual performance as measured by proficiency testing has not been well studied in sub-Saharan Africa. Therefore, we conducted an external quality assessment of laboratories engaged in transfusion screening in the region. Blinded test panels, each comprising 25 serum samples that were pedigreed for HIV, HBsAg, HCV and negative status, were sent to participating laboratories. The panels were tested using the laboratories' routine donor screening methods and conditions. Sensitivity and specificity were calculated, and multivariable analysis was used to compare performance against mode of testing, country and infrastructure. A total of 12 African countries and 44 laboratories participated in the study. The mean (range) sensitivities for HIV, HBsAg and HCV were 91·9% (14·3-100), 86·7% (42·9-100) and 90·1% (50-100), respectively. Mean specificities for HIV, HBsAg and HCV were 97·7%, 97% and 99·5%, respectively. After adjusting for country and infrastructure, rapid tests had significantly lower sensitivity than enzyme immunoassays for both HBsAg (P < 0·0001) and HCV (P < 0·05). Sensitivity also varied by country and selected infrastructure variables. While specificity was high, sensitivity was more variable and deficient in a substantial number of testing laboratories. These findings underscore the importance of proficiency testing and quality control, particularly in Africa where TTV prevalence is high. © 2014 International Society of Blood Transfusion.

  1. Prevalencia de anticuerpos contra Trypanosoma cruzi en donadores de sangre del IMSS, Orizaba, Veracruz, México Prevalence of antibodies against Trypanossoma cruzi in blood bank donors from the IMSS General Hospital in Onizaba, Veracruz, Mexico

    Directory of Open Access Journals (Sweden)

    Angel Ramos-Ligonio

    2006-02-01

    Full Text Available OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70 y un extracto crudo de epimastigotes. Las muestras fueron obtenidas entre los meses de octubre de 2001 a enero de 2002. RESULTADOS: Los 420 donadores de sangre analizados fueron seronegativos para HBV, HCV, BrA, VDRL y HIV. Después del tamizaje de los 420 donadores, se identificaron dos individuos seropositivos por las pruebas de ELISA, Western blot e IFI, con una seroprevalencia de 0.48%. CONCLUSIONES: En este estudio se muestran evidencias de seropositividad para T. cruzi en donadores de sangre del HGRO, lo que sugiere la existencia de riesgo de contaminación por transfusión sanguínea. Por tal motivo, es necesario aplicar programas para el tamizaje serológico a través de técnicas inmunológicas con alta sensibilidad y especificidad.OBJECTIVE: To estimate the prevalence of antibodies against Trypanosoma cruzi in blood donors from Hospital General Regional (HGRO of the Mexican Institute of Social Security (IMSS per its abbreviation in Spanish. MATERIAL AND METHODS: Between October 2001 and January 2002, blood samples were collected from voluntary donors at the blood bank of the Hospital General Regional of IMSS in Orizaba; Veracruz, Mexico. The samples were assayed for anti-T. cruzi by ELISA, Western blot and IFI, using a recombinant protein (MBP::Hsp70, and crude extract from epimastigotes. RESULTS: A total of 420 blood donors were studied; two of them were seropositive for ELISA, Western blot and IFI, with a seroprevalence of 0.48%. CONCLUSIONS: Some blood donors at the HGRO hospital were seropositive for T

  2. Utilización de placas de ELISA de alta y de baja avidez en la determinación de anticuerpos contra la toxina de cólera

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    Elizabeth Castañeda

    1998-06-01

    Full Text Available Los sueros de un estudio de inmunogenicidad de una vacuna oral de células enteras de Vibrio cholerae 01 más la subunidad B recombinante de la toxina (CEIsBr fueron procesados para la determinación de anticuerpos antitoxina de cólera en placas de ELlSA de alta y de baja avidez. Los títulos de anticuerpos antitoxina, de la clase IgG, de los sueros prevacunales estuvieron incrementados de 5 a 7 veces, en las placas de alta avidez, comparados con los títulos obtenidos en las placas de baja avidez. Igualmente, los títulos de los sueros post vacunales fueron 3 veces superiores cuando se realizó la misma comparación de placas. Por consiguiente, la tasa de seroconversión medida en las placas de alta avidez fue de 39%, comparada con 68% al utilizar las placas de baja avidez. El suero control de alto título de anticuerpos no detectó el problema. Se sugiere utilizar placas de baja avidez al determinar anticuerpos contra la toxina de cólera por la técnica de ELISA.

  3. Diagnóstico laboratorial de hemoglobinas semelhantes à HbS

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    Zamaro Paula Juliana Antoniazzo

    2002-01-01

    Full Text Available A hemoglobina S (HbS está presente na população brasileira com prevalência variável, dependente dos grupos raciais formadores de cada região. A migração eletroforética em pH alcalino apresenta similaridade com outras hemoglobinas, e estudos complementares para sua correta caracterização são necessários. No presente estudo objetivamos traçar um fluxograma com as metodologias disponíveis para a caracterização da hemoglobina S e das hemoglobinas que apresentam migração semelhante em pH alcalino. No período de janeiro a junho de 2000, analisamos amostras de sangue com suspeita de hemoglobina S encaminhadas ao Laboratório de Hemoglobinas da Unesp. Caracterizamos diferentes mutantes e formas interativas com hemoglobina S, por procedimentos eletroforéticos, em variados pH, análises citológicas e testes bioquímicos específicos. Os procedimentos de análise aplicados resultaram em orientação fornecida aos laboratórios de rotina sobre como proceder no diagnóstico laboratorial destas alterações de hemoglobina. Desta forma contribuímos para um melhor conhecimento sobre a variabilidade genética das hemoglobinas em nossa população, auxiliando no acompanhamento clínico e no aconselhamento genético das hemoglobinopatias com fisiopatologia relacionada à alteração.

  4. Baicalin benefits the anti-HBV therapy via inhibiting HBV viral RNAs

    International Nuclear Information System (INIS)

    Huang, Hai; Zhou, Wei; Zhu, Haiyan; Zhou, Pei; Shi, Xunlong

    2017-01-01

    Background: Although current antiviral treatments (nucleoside analogs, NAs) for chronic hepatitis B virus (HBV) infection are effective in suppressing HBV-DNA replication, their clinical outcomes can be compromised by the increasing drug resistance and the inefficiency in promoting HBsAg/HBeAg seroconversion. Objectives: In this study, we will explore possible effects and mechanism of a natural product baicalin (BA) with the anti-HBV efficacy of entecavir (ETV), a first-line anti-HBV drug, in HBV-DNA, HBsAg/HBeAg seroconversion and drug-resistance. Methods: The co-effects of BA and ETV were conducted in wild-type/NA-resistance mutant HBV cell lines and DHBV-infected duckling models. HBV-DNA/RNAs, HBsAg/HBeAg, host factors (hepatocyte nuclear factors) were explored for possible anti-HBV mechanism. Results and discussion: BA could significantly enhance and reduced HBsAg and HBeAg in hepG2.2.15, a wild-type HBV cell line. Co-treatment of BA and ETV had a more dramatic effect in NA-resistant HBV rtM204V/rtLl80M transfected hepG2 cells. Our study further revealed that BA mainly inhibited the production of HBV RNAs (3.5, 2.4, 2.1 kb), the templates for viral proteins and HBV-DNA synthesis. BA blocked HBV RNAs transcription possibly by down-regulating transcription and expression of HBV replication dependent hepatocyte nuclear factors (HNF1α and HNF4α). Thus, BA may benefit the anti-HBV therapy via inhibiting HBV viral RNAs. - Highlights: • Baicalin benefits the anti-HBV therapy. • Baicalin enhances ETV antiviral efficacy and overcomes NA-resistant HBV mutation. • The anti-HBV effect of baicalin is achieved by inhibiting HBV RNAs. • Baicalin down-regulates HBV replication-dependent host factors HNF 1α and HNF 4α.

  5. Rendimiento diagnóstico del Western Blot para detectar simultáneamente anticuerpos en pacientes con cisticercosis, hidatidosis y fascioliasis humana

    Directory of Open Access Journals (Sweden)

    Kelly Davelois

    Full Text Available Objetivo. Determinar el rendimiento diagnóstico de la técnica de Western Blot para detectar simultáneamente anticuerpos en pacientes con cisticercosis, hidatidosis y fascioliasis humana. Materiales y métodos. Estudio transversal de evaluación de prueba diagnóstica. Se obtuvieron los antígenos de excreción-secreción de las larvas de Taenia solium, quistes de Echinococcus granulosus; y la forma adulta de Fasciola hepática; que luego fueron separados electroforéticamente en geles de poliacrilamida individuales, transferidos y fijados a una membrana de nitrocelulosa para ser enfrentados con sueros de pacientes con las tres parasitosis. La sensibilidad de la técnica se evaluó empleando 300 sueros individuales, 60 pools de dos parasitosis y 20 pools de tres parasitosis y la especificidad con 75 sueros de pacientes con otras parasitosis, 10 de pacientes con otras enfermedades y 15 sueros de personas no parasitadas. Resultados. La técnica reconoció trece glicoproteínas (GP: GP 35, 31, 24, 23, 18, 17, 14 y 13 kDa para cisticercosis, GP 8,16 y 21 kDa para hidatidosis y GP: 17 y 23 kDa para fascioliasis. La prueba detectó la presencia de anticuerpos alcanzando una sensibilidad de 96% (IC95%: 94,62-98,54% en la detección de una o las trece bandas, una especificidad de 100% (IC95%: 99,50 - 100,00%; individualmente, se tuvo una sensibilidad para cisticercosis de 97% (IC95%: 93,16-100%, para hidatidosis de 94% (IC95%: 88,85-99,15% y para fascioliasis de 96% (IC95%: 91,66-100%. Conclusiones. La prueba de Western blot es eficaz en la detección, simultanea de anticuerpos en pacientes con cisticercosis, hidatidosis y fascioliasis humana, y puede ser utilizada como prueba de descarte o confirmatoria en zonas endémicas.

  6. DETERMINACIÓN DEL CATABOLISMO DE LOS ANTICUERPOS MATERNOS Y SU INTERACCIÓN CON DIFERENTES PLANES VACUNALES PARA LA ENFERMEDAD DE GUMBORO EN POLLOS DE ENGORDE

    Directory of Open Access Journals (Sweden)

    Robin O. J.

    2006-11-01

    Full Text Available Con el in de evaluar el catabolismo de los anticuerpos maternos y su interferencia con tres planes vacunales diferentes contra la enfermedad de Gumboro se realizó un estudio utilizando pollos de la estirpe Ross 308, divididos en cuatro grupos: grupo 1: control (sin vacuna; grupo 2: vacunado los días 1, 7 y 15; grupo 3: vacunados los días 1 y 12; y grupo 4: vacunado el día 12. En el primer experimento el biológico se suministró en el agua de bebida, y en el segundo se hizo en el pico. Se sacriicaron 15 aves de cada grupo a los 1, 12, 21 y 42 días de edad y se evaluó peso corporal, tamaño y peso de la bolsa de Fabricio, relación peso bolsa/peso corporal (PB/PC, grado de depleción linfoide, presencia de cepas estándar y/o variantes del virus y los títulos de anticuerpos para la enfermedad de Gumboro. El catabolismo de los anticuerpos maternos ocurrió entre los 21 y 28 días de edad. En los grupos vacunados no se evidenció una respuesta inmune activa frente a ningún plan vacunal. La relación PB/PC no indicó atroia de la bolsa en el experimento 1, aunque las lesiones histopatológicas en el último muestreo fueron grado 3; en el segundo experimento solamente el grupo 3 presentó una disminución en la relación PB/PC; las lesiones histopatológicas en el tercer y cuarto muestreos, en todos los grupos, fueron clasiicadas como grado 3 y 4. En los dos experimentos se detectó la presencia de cepas tanto clásicas como variantes y una baja respuesta humoral. Ninguno de los tres planes vacunales conirió adecuada protección a las aves.

  7. DETERMINACIÓN DEL CATABOLISMO DE LOS ANTICUERPOS MATERNOS Y SU INTERACCIÓN CON DIFERENTES PLANES VACUNALES PARA LA ENFERMEDAD DE GUMBORO EN POLLOS DE ENGORDE

    Directory of Open Access Journals (Sweden)

    R. Castañeda

    2006-01-01

    Full Text Available Con el in de evaluar el catabolismo de los anticuerpos maternos y su interferencia con tres planes vacunales diferentes contra la enfermedad de Gumboro se realizó un estudio utilizando pollos de la estirpe Ross 308, divididos en cuatro grupos: grupo 1: control (sin vacuna; grupo 2: vacunado los días 1, 7 y 15; grupo 3: vacunados los días 1 y 12; y grupo 4: vacunado el día 12. En el primer experimento el biológico se suministró en el agua de bebida, y en el segundo se hizo en el pico. Se sacriicaron 15 aves de cada grupo a los 1, 12, 21 y 42 días de edad y se evaluó peso corporal, tamaño y peso de la bolsa de Fabricio, relación peso bolsa/peso corporal (PB/PC, grado de depleción linfoide, presencia de cepas estándar y/o variantes del virus y los títulos de anticuerpos para la enfermedad de Gumboro. El catabolismo de los anticuerpos maternos ocurrió entre los 21 y 28 días de edad. En los grupos vacunados no se evidenció una respuesta inmune activa frente a ningún plan vacunal. La relación PB/PC no indicó atroia de la bolsa en el experimento 1, aunque las lesiones histopatológicas en el último muestreo fueron grado 3; en el segundo experimento solamente el grupo 3 presentó una disminución en la relación PB/PC; las lesiones histopatológicas en el tercer y cuarto muestreos, en todos los grupos, fueron clasiicadas como grado 3 y 4. En los dos experimentos se detectó la presencia de cepas tanto clásicas como variantes y una baja respuesta humoral. Ninguno de los tres planes vacunales conirió adecuada protección a las aves.

  8. [A retrospective study on HBsAg clearance rate after antiviral therapy in children with HBeAg-positive chronic hepatitis B aged 1-7 years].

    Science.gov (United States)

    Zhu, S S; Dong, Y; Xu, Z Q; Wang, L M; Chen, D W; Gan, Y; Wang, F C; Yan, J G; Cao, L L; Wang, P; Zhang, H F

    2016-10-20

    Objective: To investigate the HBsAg clearance rate after antiviral therapy in children with HBeAg-positive chronic hepatitis B (CHB) aged 1-7 years. Methods: A retrospective analysis was performed for the HBsAg clearance rate in 293 children who were hospitalized in 302 Hospital of PLA from June 2006 to December 2013, met the inclusion criteria, received antiviral therapy, and were followed up for at least 6 months after the withdrawal of antiviral therapy. The t-test or the rank sum test was applied according to the distribution of continuous data, and the chi-square test was used for comparison of categorical data. Results: The HBsAg positive rate of children's mothers was 91.1%. In the age groups of >1-≤2 years, >2-≤3 years, >3-≤4 years, >4-≤5 years, >5-≤6 years, and >6-≤7 years, the HBsAg clearance rates were 66.1%, 65.5%, 45.7%, 41.3%, 20.6%, and 27.6%, respectively. There were significant differences in HBsAg clearance rate between the age groups of >1-≤3 years and >3-≤5 years, >1-≤3 years and >5-≤7 years, and >3-≤5 years and >5-≤7 years ( P = 0.001, 0.000, and 0.008). Of all children, 64.8% were boys, among whom 41.1% achieved HBsAg clearance, and 35.2% were girls, among whom 61.2% achieved HBsAg clearance; there was a significant difference in HBsAg clearance rate between boys and girls (P = 0.001). The children with pretreatment alanine aminotransferase (ALT) levels of ≤80 IU/L, > 80 IU/L, ≤200 IU/L, and > 200 IU/L had HBsAg clearance rates of 40.7%, 51.2%, 47.6%, and 49.4%, respectively; there were no significant differences in HBsAg clearance rate between the ALT ≤80 IU/L and ALT > 80 IU/L groups and the ALT ≤200 IU/L and ALT > 200 IU/L groups ( P = 0.101 and 0.778). There was no significant difference in HBsAg clearance rate between the pretreatment HBV DNA load antiviral therapy alone and had an HBsAg clearance rate of 48.8%, and 41.3% received interferon alone for 6 months followed by lamivudine antiviral therapy and

  9. No evidence of hepatitis B virus activity in patients with anti-HBc antibody positivity with or without anti-hepatitis C virus antibody positivity.

    Science.gov (United States)

    Haushofer, Alexander C; Hauer, René; Brunner, Harald; Köller, Ursula; Trubert-Exinger, Doris; Halbmayer, Walter-Michael; Koidl, Christoph; Kessler, Harald H

    2004-04-01

    The serological pattern of anti-HBc antibody positivity without both, HBsAg and anti-HBs antibody positivity may be present in up to 4% of the population of Europe and the United States. The aim of the present study was to determine the hepatitis B virus (HBV) activity by detection of serum HBV DNA in patients with anti-HBc antibody positivity only and with confirmed anti-hepatitis C virus (anti-HCV) antibody positivity or without anti-HCV antibody positivity. A total of 141 patients positive for anti-HBc antibodies only, were investigated on serum HBV DNA load. Patients were classified into two groups: patients with confirmed positive anti-HCV antibodies (group 1) and patients without anti-HCV antibodies (group 2). Demographic data of patient groups were similar. In 66 of 70 patients with anti-HBc antibodies and anti-HCV antibodies (group 1), serum HCV RNA was detected; the remaining 4 patients were HCV RNA negative but the presence of anti-HCV antibodies was confirmed by the line probe assay. In none of the patients, with anti-HBc antibodies and without anti-HCV antibodies (group 2), serum HCV RNA was detected. In none of the patients, serum HBV DNA was detected. In this study, serum HBV DNA could not be detected in patients with anti-HBc antibodies only. There seems to be no need for determination of serum HBV DNA in patients without clinical evidence of chronic liver disease. Nevertheless, it would be useful to test patients with progressive liver disease and those, which belong to high-risk groups such as hemophiliacs, intravenous drug abusers, patients on hemodialysis, and immunocompromised patients.

  10. Hepatitis B virus surface antigen (HBsAg)-positive and HBsAg-negative hepatitis B virus infection among mother-teenager pairs 13 years after neonatal hepatitis B virus vaccination.

    Science.gov (United States)

    Yao, Qing-Qing; Dong, Xiao-Lian; Wang, Xue-Cai; Ge, Sheng-Xiang; Hu, An-Qun; Liu, Hai-Yan; Wang, Yueping Alex; Yuan, Quan; Zheng, Ying-Jie

    2013-02-01

    It is unclear whether a mother who is negative for hepatitis B virus surface antigen (HBsAg) but positive for hepatitis B virus (HBV) is at potential risk for mother-to-child transmission of HBV. This study, using a paired mother-teenager population, aimed to assess whether maternal HBsAg-negative HBV infection ((hn)HBI) is a significant source of child HBV infection (HBI). A follow-up study with blood collection has been conducted on the 93 mother-teenager pairs from the initial 135 pregnant woman-newborn pairs 13 years after neonatal HBV vaccination. Serological and viral markers of HBV have been tested, and phylogenetic analysis of HBV isolates has been done. The HBI prevalence was 1.9% (1 (hn)HBI/53) for teenage children of non-HBI mothers, compared with 16.7% (1 (hn)HBI/6) for those of (hn)HBI mothers and 2.9% (1 HBsAg-positive HBV infection [(hp)HBI]/34) for those of (hp)HBI mothers. Similar viral sequences have been found in one pair of whom both the mother and teenager have had (hn)HBI. In comparison with the (hp)HBI cases, those with (hn)HBI had a lower level of HBV load and a higher proportion of genotype-C strains, which were accompanied by differentiated mutations (Q129R, K141E, and Y161N) of the "a" determinant of the HBV surface gene. Our findings suggest that mother-to-teenager transmission of (hn)HBI can occur among those in the neonatal HBV vaccination program.

  11. Prevalencia de hepatitis viral tipo B en pacientes y personal de tres unidades de hemodiálisis en Lima.

    OpenAIRE

    BUSSALLEU, Alejandro; CIEZA, Javier; COLICHON, Alejandro; BERRIOS, Jorge

    2013-01-01

    Se estudiaron 103 pacientes y 41 miembros del personal médico y paramédico de tres centros de hemodiálisis en Lima, Perú, para determinar infección por hepatitis B. El antigeno de superficie de la hepatitis B(HBsAg) fue detectado en 21.36% de los pacientes y2.4% del personal. El anticuerpo de superficie (antiHBs) en 47.57% respectivamente. Las determinaciones de hicieron por el método de ELISA. En los pacientes la presencia de ambos marcadores no se relacionó a las transfusiones sanguíneas. E...

  12. Influence of semisynthetic modification of the scaffold of a contact domain of HbS on polymerization: role of flexible surface topology in polymerization inhibition.

    Science.gov (United States)

    Sonati, Srinivasulu; Bhutoria, Savita; Prabhakaran, Muthuchidambaran; Acharya, Seetharama A

    2018-02-01

    A new variant of HbS, HbS-Einstein with a deletion of segment α 23-26 in the B-helix, has been assembled by semisynthetic approach. B-helix of the α chain of cis αβ-dimer of HbS plays dominant role in the quinary interactions of deoxy HbS dimer. This B-helix is the primary scaffold that provides the orientation for the side chains of contact residues of this intermolecular contact domain. The design of HbS-Einstein has been undertaken to map the influence of perturbation of molecular surface topology and the flexibility of surface residues in the polymerization. The internal deletion exerts a strong inhibitory influence on Val-6 (β)-dependent polymerization, comparable to single contact site mutations and not for complete neutralization of Val-6(β)-dependent polymerization. The scaffold modification in cis-dimer is inhibitory, and is without any effect when present on the trans dimer. The flexibility changes in the surface topology in the region of scaffold modification apparently counteracts the intrinsic polymerization potential of the molecule. The inhibition is close to that of Le Lamentin mutation [His-20 (α) → Gln] wherein a mutation engineered without much change in flexibility of the contact domain. Interestingly, the chimeric HbS with swine-human chimeric α chain with multiple non-conservative mutations completely inhibits the Val-6(β)-dependent polymerization. The deformabilities of surface topology of chimeric HbS are comparable to HbS in spite of the multiple contact site mutations in the α-chain. We conclude that the design of antisickling Hbs for gene therapy of sickle cell disease should involve multiple mutations of intermolecular contact sites.

  13. Prevalencia de anticuerpos anti-Trypanosoma cruzi en mujeres en edad fértil en Socotá, Boyacá, 2014

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    Ángela Liliana Monroy

    2016-04-01

    Conclusiones. Con base en los resultados del presente estudio, se sugiere una búsqueda activa de casos de la enfermedad de Chagas en zonas no endémicas de Colombia que presenten los factores de riesgo para la adquisición de la enfermedad, aun cuando las condiciones climáticas y la altura sobre el nivel del mar sean diferentes a las descritas en la literatura científica.

  14. Anti-hepatitis B core antigen testing with detection and characterization of occult hepatitis B virus by an in-house nucleic acid testing among blood donors in Behrampur, Ganjam, Orissa in southeastern India: implications for transfusion

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    Panigrahi Rajesh

    2010-08-01

    Full Text Available Abstract Background Occult hepatitis B virus (HBV infection might transmit viremic units into the public blood supply if only hepatitis B surface antigen (HBsAg testing is used for donor screening. Our aim was to evaluate the prevalence of occult HBV infection among the HBsAg negative/antiHBc positive donations from a highly HIV prevalent region of India. Methods A total of 729 HBsAg negative donor units were included in this study. Surface gene and precore region were amplified by in house nucleic acid test (NAT for detection of occult HBV infection and surface gene was analyzed after direct sequencing. Results A total of 220 (30.1% HBsAg negative donors were antiHBc positive, of them 66 (30% were HBV DNA positive by NAT. HBV DNA positivity among 164 antiHBc only group, was 27.1% and among 40 antiHBs positive group was 30.0%. HBV/D (93.3% was predominant and prevalence of both HBV/C and HBV/A was 3.3%. Single or multiple amino acids substitutions were found in 95% samples. Conclusion Thus, a considerable number of HBV infected donors remain undiagnosed, if only HBsAg is used for screening. Addition of antiHBc testing for donor screening, although will lead to rejection of a large number of donor units, will definitely eliminate HBV infected donations and help in reducing HBV transmission with its potential consequences, especially among the immunocompromised population. The HBV genetic diversity found in this donor population are in accordance with other parts of India.

  15. Incidência pós-transfusional do HBsAg em crianças com doenças neoplásicas Post-transfusional incidence of HBsAg in children with neoplastic diseases

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    Maria Zélia Fernandes

    2009-04-01

    Full Text Available Há 350 milhões de indivíduos cronicamente infectados pelo vírus da hepatite B (VHB. O desenvolvimento e a gravidade dessa infecção dependem de vários fatores, tais como a idade da primoinfecção e resposta imune, sendo o risco dessa cronificação menor que 5% em adultos e maior que 90% em neonatos. Indivíduo cronicamente infectado pelo VHB durante a infância tem 25% de chance de morrer por cirrose ou hepatocarcinoma. Tais evidências conduziram os autores a desenvolver este estudo cujo objetivo foi estimar a incidência pós-transfusional do antígeno de superfície do vírus da hepatite B (HBsAg em crianças com neoplasias, que foram transfundidas no curso do tratamento ou seguimento da doença. Foi realizado um estudo retrospectivo, com revisão de 333 prontuários do serviço de oncologia do HIVS, de janeiro de 1993 a janeiro de 2005. Adotaram-se como critérios de inclusão: idade menor que 16 anos, diagnóstico de doença neoplásica e realização da pesquisa do HBsAg. Assim, 199 prontuários foram excluídos por não preencherem tais critérios, restando 134 que foram analisados quanto à realização de hemotransfusão. Das 134 crianças, 116 foram transfundidas e 18 não. Apresentaram pesquisa reativa para o HBsAg 32,8% das transfundidas e apenas 5,6% das não-transfundidas. O teste Exato de Fisher mostrou que houve significância estatística (p = 0,023 e observou-se que as proporções de pacientes com pesquisa reativa diferem entre os transfundidos e os não-transfundidos. O odds ratio de um paciente transfundido apresentar pesquisa reativa para o HBsAg foi calculado em 8,28 vezes maior do que um não-transfundido.There are 350 million individuals infected by the hepatitis B virus (HBV. The development and the severity of the infection depend on several factors, such as: age at the first infection and the immunity response of the subject. The risk of chronic infection is less than 5% for adults and greater than 90% for

  16. Resolution of HBV infection occurs sooner than recovery of renal disease in adult serum HBsAg-negative HBV-associated glomerulonephritis.

    Science.gov (United States)

    Xu, Fang; Wang, Chong; Shi, Xiaoju; Hou, Jie; Guo, Xiaolin; Gao, Pujun

    2018-05-02

    Most cases of hepatitis B virus-associated glomerulonephritis (HBV-GN) occur in children and present with serum HBsAg positivity. Few studies have investigated adult HBV-GN patients who are serum HBsAg-negative. This study aimed to determine the clinical and pathological features of serum HBsAg-negative adult HBV-GN patients. Clinical, pathologic and laboratory findings were collected and analyzed in a cohort of 27 adult HBV-GN patients who were serum HBsAg negative upon diagnosis. The study population included mostly men of middle age (40-59 years). Clinically, patients presented with nephrotic syndrome. Serum IgG levels were low, while serum IgM, IgA, C3, and C4 levels as well as liver and renal function tests were normal in most or all patients. Among the 27 patients, 21 tested positively for HBV antibodies. MN was the dominant pathological form on kidney biopsy. In addition, only a few patients showed a "full house" staining pattern and renal immune deposit of C1q. Serum HBsAg negative HBV-GN may represent a late stage of HBV infection. We recommend routine testing for HBV markers on renal biopsy in regions where HBV is prevalent, even when tests for serum HBV markers are negative. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  17. Prevalence of hepatitis B virus surface antigen (HBsAg) in patients undergoing extraction at the University College Hospital, Ibadan.

    Science.gov (United States)

    Odaibo, G N; Arotiba, J T; Fasola, A O; Obiechina, A E; Olaleye, O D; Ajagbe, H A

    2003-09-01

    Hepatitis B Virus (HBV) infection and its sequelae (liver chirrhosis and hepatic carcinoma) are endemic in Africa. The risk of transmission of the infection during dental treatment is real. This study was carried out to determine the rate of Hepatits B Surface Antigen (HBsAg) as a marker of hepatitis B virus infection in patients undergoing dental extraction in order to highlight the potential risk of nosocomial transmission among the Dental Health Workers (DHW) and their patients. Three hundred (143 males and 157 females) consecutive patients requiring dental extraction who volunteered were enrolled into this study. Their ages ranged from 11 years to 95 years with a mean of 37.2 years (SD = 16.725) and a median of 36 years. The overall HBsAg infection rate was 18.3% (55/300). A higher infection rate (23.1%) occurred among the male patients compared with 14% in females (p = 0.0086). The high rate of HBV infection found among this study population suggests that Dental Surgeons in this environment have a high risk of exposure to hepatitis B virus and should be immunized at the beginning of their professional life. Universal biosafety measures should be observed strictly in all invasive procedures.

  18. Baicalin benefits the anti-HBV therapy via inhibiting HBV viral RNAs.

    Science.gov (United States)

    Huang, Hai; Zhou, Wei; Zhu, Haiyan; Zhou, Pei; Shi, Xunlong

    2017-05-15

    Although current antiviral treatments (nucleoside analogs, NAs) for chronic hepatitis B virus (HBV) infection are effective in suppressing HBV-DNA replication, their clinical outcomes can be compromised by the increasing drug resistance and the inefficiency in promoting HBsAg/HBeAg seroconversion. In this study, we will explore possible effects and mechanism of a natural product baicalin (BA) with the anti-HBV efficacy of entecavir (ETV), a first-line anti-HBV drug, in HBV-DNA, HBsAg/HBeAg seroconversion and drug-resistance. The co-effects of BA and ETV were conducted in wild-type/NA-resistance mutant HBV cell lines and DHBV-infected duckling models. HBV-DNA/RNAs, HBsAg/HBeAg, host factors (hepatocyte nuclear factors) were explored for possible anti-HBV mechanism. BA could significantly enhance and reduced HBsAg and HBeAg in hepG2.2.15, a wild-type HBV cell line. Co-treatment of BA and ETV had a more dramatic effect in NA-resistant HBV rtM204V/rtLl80M transfected hepG2 cells. Our study further revealed that BA mainly inhibited the production of HBV RNAs (3.5, 2.4, 2.1kb), the templates for viral proteins and HBV-DNA synthesis. BA blocked HBV RNAs transcription possibly by down-regulating transcription and expression of HBV replication dependent hepatocyte nuclear factors (HNF1α and HNF4α). Thus, BA may benefit the anti-HBV therapy via inhibiting HBV viral RNAs. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Títulos de anticuerpos antisulfátido en pacientes con cardiopatía chagásica crónica y con otras formas de cardiopatía

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    Rando García

    1998-04-01

    Full Text Available Aún no se ha podido encontrar un tratamiento específico para la enfermedad de Chagas, a pesar de su endemicidad en extensas zonas de la Región de las Américas. En estudios anteriores se ha propuesto la posibilidad de que el galactocerebrósido sulfatado presente en Trypanosoma cruzi podría actuar como inmunógeno para producir las concentraciones elevadas de anticuerpos antisulfátido que se han encontrado en pacientes con infección crónica por ese parásito. Ese fenómeno podría ser importante en la patogenia de los síntomas cardíacos o del sistema nervioso periférico que caracteriza a la enfermedad de Chagas, que es la primera causa de miocarditis en Centroamérica y América del Sur y la segunda causa de insuficiencia cardíaca en algunos países de esas subregiones. El presente estudio se realizó con objeto de determinar si había diferencia entre pacientes con la enfermedad de Chagas y otros pacientes sin la enfermedad con respecto a la presencia de anticuerpos antisulfátido y describe las concentraciones de anticuerpos antisulfátido que se encontraron en 124 pacientes (74 hombres y 50 mujeres de 15 a 94 años de edad hospitalizados en el Servicio de Cardiología del Hospital Vargas de Caracas, durante el período del 1 de julio de 1993 al 30 de junio de 1995. Los títulos de anticuerpos antisulfátido se midieron con la técnica del inmunoensayo enzimático (ELISA usando como antígeno cerebrósido sulfatado obtenido de cerebro de bovino. De los 124 pacientes estudiados, 39 (31,5% eran chagásicos y mostraron títulos de anticuerpos antisulfátido más altos que los 85 no chagásicos (P = 0,0298 y que un grupo control de 28 sujetos aparentemente sanos (P = 0,0035. Las concentraciones séricas de anticuerpos antisulfátido en pacientes con otras formas de cardiopatía también se compararon con las del grupo control y se encontraron títulos significativamente altos en sujetos con cardiopatía isquémica aguda (P = 0

  20. Estandarización de una prueba de ELISA para detectar anticuerpos IgE en pacientes con equinococosis quistica y su utilidad en el diagnóstico y seguimiento de pacientes tratados con albendazol: reporte preliminar

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    Herman Vildózola

    2012-01-01

    Full Text Available Objetivos: Determinar las diluciones y concentraciones optimas de una prueba de ELISA para detectar anticuerpos IgE, así como su sensibilidad, espécificidad y valor predictivo en pacientes con equinococosis quistica. Analizar si los niveles de anticuerpos IgE especificos se correlacionan con la respuesta al tratamiento médico con albendazol en un periodo mayor a un año de finalizado el tratamiento. Diseño: Estudio cuasiexperimental con grupo control. Institución: Instituto de Medicina Tropical Daniel A. Carrión, Facultad de Medicina, UNMSM, Lima, Perú. Material de estudio: Prueba de ELISA para anticuerpo IgE. Intervenciones: Estandarizacion de la prueba de ELISA para anticuerpo IgE y diseño preexperimento con preprueba y posprueba en un solo grupo, para evaluar su valor en el diagnóstico y seguimiento postratamiento de pacientes con quiste hidatidico hepatico tratados con albendazol. Para la estandarizacion de la prueba de ELISA, se utilizó suero de cinco pacientes con diagnóstico clínico de equinococosis quistica, y la sensibilidad y especificidad de la prueba se usó suero de 30 pacientes aparentemente sanos. Para determinar las reacciones cruzadas, se utilizó 16 muestras de suero de pacientes con otras helmintiasis (ascariasis, strongiloidiasis, toxocariosis, trichuriasis, himenolepiasis, cisticercosis y teniasis. Para el diagnóstico y seguimiento postratamiento de equinocococosis quística, se utilizó el suero de 17 pacientes. Principales medidas de resultados: Sensibilidad y especificidad de prueba estandarizada de ELISA para detectar anticuerpos IgE. Resultados: La prueba estandarizada de ELISA para detectar anticuerpos IgE tuvo una sensibilidad de 95,6% y una especificidad de 100%. En los pacientes con quiste hidatídico hepático considerados curados, uniformemente disminuyeron los niveles de anticuerpo tipo IgE hasta la negativización. Se obtuvo elevación de los niveles de IgE en los pacientes que presentaron

  1. Bajo índice de sospecha para deficiencia de anticuerpos en niños sometidos a procedimientos quirúrgicos de Otorrinolaringología

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    AB Ramírez-López

    2016-06-01

    Full Text Available INTRODUCCIÓN: las inmunodeficiencias primarias están subdiagnosticadas en todo el mundo, aun en centros de tercer nivel de atención. Los defectos de anticuerpos constituyen el grupo más prevalente y suelen manifestarse clínicamente después de los 6 meses de edad como infecciones respiratorias recurrentes causadas por bacterias encapsuladas. Algunas cirugías de oído-nariz y garganta se indican en pacientes con infecciones respiratorias recurrentes o complicadas que no han respondido adecuadamente al tratamiento médico. Estos niños sometidos a procedimientos quirúrgicos luego de una historia de infecciones respiratorias recurrentes pueden constituir un grupo de alto riesgo para inmunodeficiencias primarias.   OBJETIVO: indagar qué tan frecuentes son los defectos de anticuerpos en niños sometidos a cualquiera de tres cirugías de otorrinolaringología.   MATERIALES Y MÉTODOS: revisamos los expedientes electrónicos de niños que hubieran sido sometidos a adenoamigdalectomía (ATT, drenaje endoscópico de senos paranasales (ESD y colocación de tubos timpánicos de ventilación (TVT en nuestro instituto durante 2011-2012, en busca de niveles séricos de inmunoglobulinas (IgE, IgG, IgA, IgM.   RESULTADOS: encontramos 112 procedimientos quirúrgicos en 87 pacientes, de los cuales la indicación para cirugía fue infecciosa en 37 (21 varones, 1 muerto, edad media 7.3 años, más frecuentemente rinosinusitis (19/37 y amigdalitis (9/37 crónicas. Los procedimientos incluyeron: 24 adenoamigdalectomías, 13 drenajes endoscópicos de senos paranasales y 6 colocaciones de tubos timpánicos de ventilación, para un total de 43; ocho pacientes (21.6% fueron sometidos a más de una cirugía. La IgE sérica se encontró en el expediente de 27 pacientes (72.9%, y “al menos IgG” en 18 (48.6%. Sólo 70% de las pruebas fueron ordenadas antes de la cirugía. Los resultados anormales incluyeron: IgE elevada en 10/27, Ig

  2. The variability of hepatitis B envelope is associated with HBs antigen persistence in either chronic or acute HBV genotype A infection.

    Science.gov (United States)

    Eschlimann, Marine; Malvé, Brice; Velay, Aurélie; Fenaux, Honorine; Berger, Sibel; Frippiat, Jean-Pol; Zoulim, Fabien; Bensenane, Mouni; Bronowicki, Jean-Pierre; Goehringer, François; May, Thierry; Jeulin, Hélène; Schvoerer, Evelyne

    2017-09-01

    More than 240 million people are chronically infected by hepatitis B virus (HBV) worldwide. Envelope proteins play a crucial role in viral cellular entry and immune recognition. The loss of HBs antigen (HBsAg) correlated with a good clinical prognosis is rarely achieved with or without treatment (3-16%). HBV envelope variability was investigated according to HBsAg persistence. The cohort consisted of 15 HBV genotype A-infected patients divided into "resolvers", with HBsAg clearance, and "non-resolvers", with HBsAg persistence and in subgroups: acute (n=5, AHBV) or chronic infection (n=4, CHBV) and HBV/HIV coinfection (n=6, CHBV/HIV). HBV S and preS sequences were studied by direct and ultra-deep sequencing. Amino acid sequences were analyzed with bioinformatics for predicted antigenicity. In S gene, the complexity was lower in AHBV than in chronic-infected patients (p=0.046). Major mutations, detected using direct sequencing, were more frequent in AHBV developing chronicity (p=0.01) than in AHBV resolvers. In the Major Hydrophilic Region, more frequent mutations were observed in non-resolvers versus resolvers (p=0.047) and non-resolvers tended to have more haplotypes with a reduced predicted antigenicity (p=0.07). Most of the mutations in preS/S region were found rather in epitopic than in non-epitopic areas (p=0.025). Interestingly, the mutation sY161F found in 3/8 non-resolvers was associated with a decrease in predicted antigenicity (28%; AnTheProt). HBsAg persistence was correlated with mutations and deletions in areas playing a key role in immune recognition. These data suggest that variability in HBV envelope could favor immune escape in various clinical settings of HBV genotype A-infected patients. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Detection of HbsAg and hATIII genetically modified goats (Caprahircus) by loop-mediated isothermal amplification.

    Science.gov (United States)

    Tao, Chenyu; Zhang, Qingde; Zhai, Shanli; Liu, Bang

    2013-11-01

    In this study, sensitive and rapid detection systems were designed using a loop-mediated isothermal amplification (LAMP) method to detect the genetically modified goats. A set of 4 primers were designed for each exogenous nucleic acids HBsAg and hATIII. The DNA samples were first amplified with the outer and inner primers and released a single-stranded DNA,of which both ends were stem-loop structure. Then one inner primer hybridized with the loop, and initiated displacement synthesis in less than 1 h. The result could be visualized by both agarose gel electrophoresis and unaided eyes directly after adding SYBR GREEN 1. The detection limit of LAMP was ten copies of target molecules, indicating that LAMP was tenfold more sensitive than the classical PCR. Furthermore, all the samples of genetically modified goats were tested positively by LAMP, and the results demonstrated that the LAMP was a rapid and sensitive method for detecting the genetically modified organism.

  4. Anti-tumor and anti-virus activity of polysaccharides extracted from Sipunculus nudus(SNP) on Hepg2.2.15.

    Science.gov (United States)

    Su, Jie; Jiang, Linlin; Wu, Jingna; Liu, Zhiyu; Wu, Yuping

    2016-06-01

    Many polysaccharides have biological activities and have been investigated for their antitumor effects. In this study, we investigated the anti-tumor activity and anti-virus activity of SNP-the water-soluble polysaccharides extracted from Sipunculus nudus on Hepg2.2.15. Flow cytometry analysis demonstrated that SNP induced dose-dependent cell apoptosis on Hepg2.2.15. Real-time PCR and Western Blot analysis showed that SNP down-regulated the synthesis of HBsAg, HBV-DNA and enhanced the expression of pro-apoptosis proteins TNF-α, caspase-3, and Bax, while decreasing the expression of the anti-apoptosis proteins survivin, Bcl-2, and VEGF. These results suggested that SNP suppressed cell viability of Hepg2.2.15 and that could be a novel anti-tumor and anti-HBV agent. Copyright © 2016. Published by Elsevier B.V.

  5. Determinación de Anticuerpos IgM contra Virus dengue partir de sangre absorbida en papel filtro: Un método alternativo y sencillo

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    María García M

    2000-01-01

    Full Text Available Objetivo: Se propone como alternativa la obtención de muestras de sangre total en papel filtro, para la determinación de anticuerpos IgM contra dengue, por ser un método de recolección sencillo y no requerir de muchos cuidados en el envío al laboratorio. Materiales y métodos: De 100 pacientes con diagnóstico clínico de dengue clásico, se obtuvieron en forma simultánea, muestras de suero en tubos al vacío y de sangre total en papel filtro. Ambas muestras fueron evaluadas con el método serológico ELISA Captura de IgM a los 30 días de obtenida la muestra. Resultados: De las 100 muestras 25 fueron positivas y 75 negativas en suero, y 24 positivas y 74 negativas en papel filtro. Se obtuvo una concordancia por índice Kappa de 0,97, sensibilidad y especificidad de 96,0% y 98,0% respectivamente; el valor predictivo positivo fue 96,0%, y valor predictivo negativo fue 98,0%. Conclusión: Se evidencia muy buena sensibilidad, especificidad y concordancia en la determinación de IgM contra dengue, al utilizar papel filtro en la obtención de muestra de sangre.

  6. Relevamiento serológico de anticuerpos contra enfermedades virales de interés sanitario en llamas (Lama glama de la provincia de Jujuy, Argentina

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    Elena S Barbieri

    Full Text Available Las poblaciones de llamas de Argentina se concentran principalmente en la provincia de Jujuy; su explotación representa un importante recurso económico de las comunidades altoandinas. El objetivo de este trabajo fue evaluar la seroprevalencia de anticuerpos contra algunos agentes virales asociados a enfermedades de impacto productivo en rodeos de llamas de Jujuy. Se analizaron 349 sueros de llamas adultas de 6 departamentos de la puna jujeña ubicados por encima de los 3300 msnm. Se obtuvo una prevalencia del 100 % para rotavirus grupo A y del 70 % para el virus parainfluenza-3 bovino, mientras que no se detectaron reactores para herpesvirus bovino 1, virus de la diarrea viral bovina, influenza A humana (H1N1 e influenza equina (H3N8. Los resultados obtenidos confirman la amplia distribución de rotavirus y virus parainfluenza y la baja susceptibilidad a herpesvirus y pestivirus en las tropas de llamas de la puna jujeña.

  7. Historic and current hepatitis B viral DNA and quantitative HBsAg level are not associated with cirrhosis in non-Asian women with chronic hepatitis B.

    Science.gov (United States)

    Harkisoen, S; Arends, J E; van den Hoek, J A R; Whelan, J; van Erpecum, K J; Boland, G J; Hoepelman, A I M

    2014-12-01

    Some studies done in Asian patients have shown that serum levels of hepatitis B virus (HBV) DNA predict the development of cirrhosis. However, it is unclear whether this also applies for non-Asian patients. This study investigated historic and current HBV DNA and quantitative hepatitis B surface antigen (HBsAg) levels as predictors of cirrhosis in non-Asian women with chronic HBV. A retrospective cohort study of non-Asian women with chronic HBV was performed. Among other variables, HBV DNA and quantitative HBsAg levels were measured in stored historic serum samples obtained during pregnancy (period 1990-2004) and current serum samples (period 2011-2012) to determine any association with liver cirrhosis by liver stiffness measurement (LSM). One hundred and nineteen asymptomatic, treatment-naïve non-Asian women were included; the median number of years between the historic sample and the current sample was 17 (interquartile range (IQR) 13-20). The median historic log HBV DNA and quantitative log HBsAg levels were 2.5 (IQR 1.9-3.4) IU/ml and 4.2 (IQR 3.6-4.5) IU/ml, respectively. LSM diagnosed 14 patients (12%) with F3-F4 fibrosis, i.e. stiffness >8.1kPa. No association of cirrhosis was found with historic HBV DNA (relative risk (RR) 0.34, 95% confidence interval (CI) 0.05-2.44) or with the quantitative HBsAg level (HBsAg level >1000 IU/ml, RR 0.35, 95% CI 0.11-1.11). Multivariable analysis identified alcohol consumption (odds ratio (OR) 6.4, 95% CI 1.3-30.1), aspartate aminotransferase >0.5 times the upper limit of normal (OR 15.4, 95% CI 1.9-122.6), and prothrombin time (OR 12.0, 95% CI 1.2-120.4), but not HBV DNA or quantitative HBsAg level, to be independent predictors of the presence of cirrhosis. Neither historic nor current HBV DNA or the quantitative HBsAg level is associated with the development of HBV-related cirrhosis in non-Asian women. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

  8. Impact of "a" determinant mutations on detection of hepatitis B surface antigen (HBsAg) in HBV strains from Chinese patients with occult hepatitis B.

    Science.gov (United States)

    Huang, Xiangyan; Ma, Chenyun; Zhang, Qiang; Shi, Qingfen; Huang, Tao; Liu, Chao; Li, Jie; Hollinger, F Blaine

    2017-10-01

    This study was designed to detect mutations that occur within the "a" determinant in the S gene of the hepatitis B virus (HBV) in patients with occult hepatitis B (OHB), and to analyze the influence of these mutations on expression and reactivity of the hepatitis B surface antigen (HBsAg). Twenty-three certified OHB samples were compared to 32 HBsAg positive samples from patients with chronic hepatitis B. The median HBV DNA levels in the OHB group were significantly lower than those in the control group (P determinant were analyzed by gene amplification and sequencing. This revealed mixed infections in which clones within a sample displayed either different mutations or mutations in association with clones that exhibited wild type amino acid patterns. Sequencing analysis also showed a significant difference between the proportions of amino acid mutations observed in the OHB and control groups. Seven recombinant S (rS) proteins with corresponding OHB mutations and three wild type alleles were expressed and purified in the Pichia pastoris expression system to preserve conformational attributes, and their reactivity analyzed using six commercial HBsAg assays. The OHB sera were HBsAg nonreactive while the rS proteins with corresponding OHB mutations were universally reactive. Thus, we postulate that the reduced binding affinity between mutated HBsAg and its antibody may not be as important in defining OHB as is the effect of specific mutations in the preS/S region of the genome that affect the synthesis and secretion of the S protein and/or the virion. © 2017 Wiley Periodicals, Inc.

  9. Los complejos Chaperonina(MSP63inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica.

    Directory of Open Access Journals (Sweden)

    Juan Marzoa

    2009-08-01

    Full Text Available Alteration of the native structure of antigens can lead to the loss of protective epitopes. Our previous results showed that separation of the meningococcal outer membrane proteins in native conditions revealed the existence of protein complexes that could be relevant for the development of new vaccine formulations. The aim of this work was to analyse the immunogenic characteristics of a highly conserved 700 kDa chaperonin complex (CxChap detected and purified by using high resolution clear native electrophoresis. Analysis of the anti-CxChap serum by Western-blotting revealed the presence of antibodies against the MSP63 but also against the macrophage infectivity potentiator-like protein (MIP, which is coopurified with the chaperonin complex. Antibodies raised by immunisation with CxChap chaperonin complex show bactericidal and opsonophagocytic activity.

  10. Hepatitis B virus activity in patients with anti-hepatitis C virus antibody positivity and hepatitis B antigen positivity.

    Science.gov (United States)

    Haushofer, Alexander C; Hauer, René; Brunner, Harald; Köller, Ursula; Trubert-Exinger, Doris; Halbmayer, Walter Michael; Haas, Josef; Kessler, Harald H

    2002-12-01

    Co-infection with hepatitis B virus (HBV) and HCV seems to be relatively frequent. There might be a mutual influence on replication activity of HBV and HCV. To determine the HBV activity in patients with serum HCV RNA and HBsAg positivity and in those with confirmed anti-HCV antibody and HBsAg positivity but serum HCV RNA negativity. A total of 1,200 anti-HCV antibody positive samples were investigated. Samples of HCV RNA and HBsAg positive patients were compared with those of confirmed anti-HCV and HBsAg positive but serum HCV RNA negative patients. HBV activity was tested with the quantitative Cobas Amplicor HBV Monitor Test (Roche Diagnostic Systems, Pleasanton, CA). Of all studied patients with chronic hepatitis C (serum HCV RNA positivity) only 1.0% were found to be HBsAg positive. In contrast, of all patients with confirmed anti-HCV positivity but serum HCV RNA negativity, 11.9% tested HBsAg positive. The median of HBV DNA levels of patients with serum HCV RNA positivity and HBeAg seroconversion (4.0 x 10(2) HBV DNA copies per ml) was found to be slightly lower than that of patients with serum HCV RNA negativity and HBeAg seroconversion (2.5 x 10(3) HBV DNA copies per ml; P>0.05). The median of HBV DNA levels of patients with serum HCV RNA positivity but without HBeAg seroconversion (1.1 x 10(4) HBV DNA copies per ml) was found to be significantly lower than that of patients with serum HCV RNA negativity but without HBeAg seroconversion (2.6 x 10(7) HBV DNA copies per ml; P<0.05). A mutual effect on HBV and HCV replication could be observed. The molecular assay for quantification of serum HBV DNA was found to be useful for the routine diagnostic laboratory.

  11. Séroprévalence de l’AgHBs chez la femme enceinte dans le centre du Maroc

    Science.gov (United States)

    Sbiti, Mohammed; Khalki, Hanane; Benbella, Imane; louzi, Lhoussaine

    2016-01-01

    La transmission materno-fœtale du virus de l'hépatite B (VHB) est un problème qui préoccupe toujours les autorités sanitaires à travers le monde et suscite la mise en place de mesures préventives renforcées. Le statut réplicatif du virus chez la mère séropositive, évalué par la positivité de l'AgHbe et le taux de la charge virale, conditionne le risque de contamination qui est plus important en période péri-natale. Les mères porteuses chroniques du VHB constituent un véritable réservoir de la transmission verticale de cette infection. Nous avons étudié, à travers cette enquête sur 1120 femmes enceintes Marocaines, la séroprévalence de l'AgHBs qui était de 2,35%, dans le but d'alimenter les données nationales sur ce sujet. Parmi ces femmes séropositives pour l'AgHBs, 79,1% avaient un AgHBe négatif et ont bénéficié d'une recherche moléculaire qui s'est révélée positive dans 89,4% des cas. La vaccination de la femme en âge de procréer est l'un des piliers majeurs de la prévention de la transmission verticale du VHB, seulement 2,4% de nos patientes étaient vaccinées. Ceci relève l'intérêt du dépistage au cours de la grossesse, qui doit se focaliser sur la sensibilisation à la vaccination de femmes séronégatives, et le suivi par biologie moléculaire des mères séropositives dans le but d'instaurer un traitement prophylactique adéquat. PMID:27795784

  12. Seroprevalencia de anticuerpos bactericidas frente al meningococo C en Cantabria 10 meses tras la campaña de vacunación

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    Aledo Linos Alvaro Gonzalez de

    1998-01-01

    Full Text Available FUNDAMENTO: En febrero-marzo de 1997 se realizó en Cantabria (Comunidad Autónoma del Estado Español con 541.885 habitantes, de los cuales 107.787 tienen una edad entre 18 meses y 19 años una campaña de vacunación antimeningocócica dirigida a niños entre 18 meses y 19 años, que abarcó a todos sus municipios y alcanzó una cobertura mayor del 95%. En los 12 meses posteriores la eficacia vacunal fue del 95.68% en todas las edades. Para ayudar a decidir una revacunación se realizó un estudio de seroprevalencia de anticuerpos bactericidas en la población vacunada. MÉTODOS: en diciembre de 1997 se analizaron muestras de sangre de 414 niños vacunados, obtenidas al azar de extracciones oportunistas realizadas en Atención Primaria y en Hospitales Públicos de la Comunidad, y de niños escolarizados en las guarderías públicas dependientes de la Dirección General de Bienestar Social de Cantabria. Se analizó la tasa de anticuerpos bactericidas en el Centro Nacional de Microbiología, estableciéndose el nivel de "efecto vacunal" en la dilución 1/8. RESULTADOS: se obtuvieron los siguientes porcentajes de títulos > o = 1/8 (entre paréntesis, grupos de edad al vacunarse: 0% (18-24 meses, 4% (1.5 a 4 años, 7.1% (1.5 a 6 años, 51.3% (6 a 12 años y 81.2% (12 a 19 años. Debido a que la definición de "efecto vacunal" se cifró en la dilución 1/8 de forma artificial, y a que otros trabajos la cifran en 1/4, en 287 sueros con resultado <1/8 se repitió el ensayo bactericida con la dilución 1/4, resultando que 286 (el 99.6% también dieron negativo. Es decir, que el resultado final no variaría si situásemos el punto de corte en la dilución 1/4 en vez de en 1/8. No se encontraron diferencias significativas según la procedencia de municipios donde hubiera habido casos de meningitis C o no los hubiera habido. CONCLUSIONES: la actividad bactericida es muy baja en los menores de 4-6 años, inferior a lo publicado, y mayor por encima de esa

  13. Seroprevalencia de anticuerpos contra el poliovirus 1 en niños mexicanos Seroprevalence of antibodies against poliovirus type 1 in Mexican children

    Directory of Open Access Journals (Sweden)

    Juan Ruiz-Gómez

    2007-01-01

    Full Text Available OBJETIVO: Analizar la frecuencia y distribución de la prevalencia de anticuerpos contra el virus de la poliomielitis tipo 1 en niños menores de 10 años en México, además de contribuir a la evaluación del programa de vacunación. MATERIAL Y MÉTODOS: Se estudió la presencia de anticuerpos contra el poliovirus tipo 1 en una muestra de la Encuesta Nacional de Salud 2000. Los sueros se recolectaron entre noviembre de 1999 y junio de 2000 a nivel nacional. La muestra constó de 6 270 niños de uno a nueve años de edad y se utilizó la técnica de neutralización. RESULTADOS: La seropositividad fue de 99.3% (IC95%99.1-99.7. Se identificaron como factores de riesgo de susceptibilidad el analfabetismo (RM= 1.5, p= 0.002 y el bajo ingreso familiar (RM= 1.4, p= 0.0487 y como factor protector el acceso a la seguridad social (RM= 0.41, p=0.04. CONCLUSIONES: Las actividades del programa de vacunación que han llevado a cabo las instituciones de salud han dado resultados en el control y eliminación de la enfermedad. Sin embargo, los programas de vacunación no deben interrumpirse, incluso si se ha registrado 99.3% de seropositividad; no puede soslayarse que al convertir el 0.7% restante, se calcula que hay 190 000 niños susceptibles de contraer la enfermedad. Estos niños se localizan sobre todo en el sur del país.OBJECTIVE: To analyze the frequency and distribution of the prevalence of antibodies against the poliomyelitis type 1 virus in children 1-9 years old in Mexico. MATERIAL AND METHODS: Antibodies against poliovirus type 1 (neutralization method were studied in 6 270 sera selected from the 24 232 sera from children one to nine years old, collected by the 2000 National Health Survey (ENSA 2000 that was conducted from November 1999 to June 2000. RESULTS: Overall seroprevalence was 99.3% (95%CI: 99.1-99.7. Using bivariate analysis, absence of antibodies was shown to be associated with illiteracy (OR= 1.5, p=0.002 and low household income (OR= 1

  14. Determinación de anticuerpos contra paracoccidioides brasiliensis e histoplasma capsulatum en una población de mamíferos silvestres

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    Jeannette Castillo

    1989-06-01

    Full Text Available En una revisión reciente sobre la ecología del Paracoccidioides brasiliensis, Restrepo (1 señala lo mucho que se desconoce sobre la relación entre el hongo y su medio ambiente. No obstante que el aislamiento del P. brasiliensis del suelo ha sido informado en la literatura solamente en dos ocasiones y de dos diferentes áreas endémicas (2, 3 se acepta, en general, que el suelo constituye el microhábitat del hongo. También se ha considerado el papel de los animales silvestres en la ecología del hongo aunque los informes presentados en la literatura han sido muy discutidos, tanto el que se refiere a murciélagos (Artibeus literatus (4, 5 como el que trata de un mono boliviano (Saimiri sciureus (6. Sin embargo, en un estudio realizado recientemente en el estado de Pará, Brasil, al hacer un levantamiento de mamíferos del área, dirigido a detectar hospederos silvestres de Leishmania, se informó el aislamiento de P. brasiliensis de órganos de armadillos (Dasypus novemcinctus (7. Este hallazgo fue repetido posteriormente por el mismo grupo, empleando una población mayor de estos animales (8. Debido a que el grupo de Parasitología del INS estaba realizando un estudio similar al mencionado sobre mamíferos silvestres en Leishmania y a que se habían obtenido sueros de tales animales silvestres, decidimos determinar la presencia de anticuerpo contra P. brasiliensis e Histoplasma capsulatum var capsulatum con el objeto de establecer la posible infección natural con estos dos agentes.

  15. Nivel de corte de los ELISAs para cuantificación de anticuerpos inducidos por la vacuna antimeningocócica VA-MENGOC-BC

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    Rolando Ochoa

    2001-06-01

    Full Text Available Para medir el grado de protección inducido por vacunas antimeningocócicas se ha establecido el Ensayo Bactericida en Suero (EBS y se perfeccionan otros ensayos inmunobiológicos, sin embargo, es necesario contar con pruebas sencillas como el ELISA, capaz de evaluar un gran número de muestras. Se estimó el nivel de corte de los ELISAs para la cuantificación de IgG humana contra los antígenos de VA-MENGOC-BC, vacuna antimeningocócica compuesta por vesículas proteicas de membrana externa de meningococo B y polisacárido capsular de meningococo C, con respecto a un panel de muestras de suero de lactantes, caracterizado por Ensayo Bactericida en Sangre Total (EBST. Los valores correspondientes a la máxima sensibilidad y especificidad fueron respectivamente; 2 μg/mL y 12 μg/mL para antipolisacárido C, y 1000 U/mL y 7000 U/mL para antiproteínas de membrana externa. La mayor coincidencia se obtuvo con 6 μg/mL y 2500 U/mL. Se evaluó otro panel de muestras de suero de adolescentes entre 14 y 18 años, por ELISA y EBS para Neisseria meningitidis serogrupos B y C, alcanzándose una buena concordancia. Doce años después de la inmunización con VA-MENGOC-BC persiste una importante concentración de anticuerpos contra los antígenos vacunales en los sueros estudiados.

  16. Prueba de Elisa indirecta para la detección de anticuerpos IgM para el diagnóstico de Leptospirosis humana

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    Manuel Céspedes Z

    2002-01-01

    Full Text Available Para el diagnóstico temprano de enfermedades con cuadro clínico inespecífico como la leptospirosis, es necesario la confirmación laboratorial mediante pruebas específicas, con la finalidad de que el diagnóstico sea más acertado y rápido. Objetivo: se realizó un estudio comparativo entre la prueba de microaglutinacion (MAT y la prueba de ELISA indirecta estandarizada con un "pool" de antígenos de Leptospira interrogans, para la detección de anticuerpos IgM, en muestras de suero de fase aguda de leptospirosis humana. Materiales y métodos: 40 muestras de pacientes con sospecha clínica y con títulos de 1:100-1:12800 por la prueba de MAT, 80 muestras negativas de pacientes aparentemente sanos con enfermedades como Brucelosis, Sífilis, Tifus murino, Hepatitis B, Fiebre Amarilla, Dengue y Enfermedad de Carrión fueron evaluados por ELISA IgM. Resultados: se obtuvo una sensibilidad de 97,5% y especificidad de 98,75%, no observándose reacción cruzada con otras enfermedades. Conclusión: ELISA IgM validado en el laboratorio es suficiente sensible, específico y de fácil aplicación para el uso como prueba de tamizaje en una infección por Leptospiras con la subsecuente confirmación por MAT.

  17. Producción de anticuerpos policlonales IgG contra la proteina iduronato-2-sulfato sulfatasa y desarrollo de un sistema de detección para IDS humana recombinante.

    Directory of Open Access Journals (Sweden)

    Olga Peña

    2005-06-01

    Full Text Available Introducción. La enfermedad de Hunter es un trastorno lisosómico caracterizado por la deficiencia de la enzima iduronato-2-sulfato sulfatasa (IDS (EC 3.1.6.13. Esta enfermedad, al igual que muchos trastornos metabólicos, son patologías intratables mediante la terapéutica convencional; sin embargo, existe la posibilidad de ser tratada alternativamente mediante terapia génica o terapia de reemplazo enzimático. Objetivo. El Instituto de Errores Innatos del Metabolismo (IEIM ha desarrollado un sistema de expresión de sulfatasas para producir IDS humana recombinante (IDShr en Escherichia coli y Pichia pastoris, con resultados favorables. El objetivo principal de este trabajo fue desarrollar un sistema de detección de IDS humana recombinante. Materiales y métodos. Para el efecto, se inmunizaron con IDS comercial de TKT (Cambridge, MA dos conejos de raza Nueva Zelanda blanca y los anticuerpos purificados a partir del suero se utilizaron en el desarrollo de una técnica semicuantitativa por dot-blot. Diferentes muestras de extractos crudos de fermentaciones con P. pastoris y E. coli se procesaron con el fin de poder determinar la presencia de la enzima. Resultados. Se demostró que los anticuerpos eran específicos en el reconocimiento de la IDS sin presentar reactividad cruzada con proteínas contaminantes de los extractos crudos. Conclusión. Por consiguiente, los anticuerpos se podrán usar en el desarrollo de una técnica ELISA tipo sandwich como método de detección y cuantificación de la enzima y en procesos de purificación de la misma mediante cromatografía de afinidad.

  18. Construcción y expresión de una proteína de fusión C-terminal entre la cadena kappa del anticuerpo 14D9 y una proteína fluorescente roja

    OpenAIRE

    González, Cintia Daniela

    2010-01-01

    El objetivo general fue la construcción de una inmunoglobulina reportera y su expresión en plantas a fin de evaluar la factibilidad de generar un reportero funcional. Los objetivos específicos fueron: Objetivo 1: Diseñar y construir una fusión del gen codificante para la cadena liviana del anticuerpo 14D9 a proteínas fluorescentes (kappa-PF). Objetivo 2: Estudiar la síntesis y localización subcelular de la fusión Kappa-PF por expresión temporal. Objetivo 3: Estudiar la síntesis y ...

  19. Urticaria crónica con alteraciones de la función tiroidea y anticuerpos antiperoxidasa tiroidea Chronic urticaria with alterations of the thyroid function and thyroid peroxidase antibodies

    OpenAIRE

    Diego S. Fernández Romero; Alejandro Malbrán

    2005-01-01

    La urticaria crónica es una enfermedad frecuente, caracterizada por la presencia de ronchas y/o angioedema con una duración superior a las 6 semanas. En un número importante de pacientes se comporta como una enfermedad autoinmune asociada frecuentemente con alteraciones en la función tiroidea y con la presencia de anticuerpos antitiroideos. Presentamos una serie de 70 pacientes consecutivos con diagnóstico de urticaria crónica a los cuales les investigamos la función tiroidea y la presencia d...

  20. Estudio de la función de la desaminasa inducida por activación en la diversificación de anticuerpos y en la generación de lesiones linfomagénicas

    OpenAIRE

    Vidal Sernández, Isora

    2011-01-01

    La Desaminasa Inducida por Activación inicia las reacciones de Hipermutación Somática (SHM) y Cambio de Isotipo (CSR), responsables de la diversificación secundaria de anticuerpos. Además, AID promueve la generación de translocaciones cromosómicas con potencial linfomagénico. Por ello, el estudio de la regulación de esta enzima es de vital importancia. En primer lugar, realizamos un análisis exhaustivo de las consecuencias de una reducción en los niveles de AID in vivo e in vitro utilizando a...

  1. Prevalence of anti-hepatitis B surface antibodies among children and adolescents vaccinated in infancy and effect of booster dose administered within a pilot study.

    Science.gov (United States)

    Bassal, R; Markovich, M P; Weil, M; Shinar, E; Carmeli, Y; Dan, M; Sofer, D; Mendelson, E; Cohen, D; Shohat, T

    2017-10-01

    We determined the prevalence of anti-hepatitis B surface antibodies (anti-HBs) among children and adolescents vaccinated for hepatitis B virus in infancy as part of the routine vaccination programme. A representative serum sample of the Israeli population age 0-19 was tested. In a separate pilot study, a booster dose of hepatitis B vaccine was administered to 31 candidates for national service, who were fully vaccinated in infancy and tested negative for hepatitis B surface antibodies at age 17-19 years and anti-HBs antibodies were assessed 8 weeks later. Of the 1273 samples tested, 631 (49·6%) were positive to anti-HBs antibodies. Seropositivity rates were 89·5% among infants aged 6-12 months and declined significantly with age to 20·7% at age 19 years. No differences in seropositivity rates were observed between Jews and Arabs, males and females and those born in Israel and in other countries. Seroconversion rate among the 31 individuals who received a booster dose was 90·3% (95% CI: 75·1-96·6%). We recommend a booster dose for healthcare personnel before starting to work at the health care facility.

  2. Core I97L mutation in conjunction with P79Q is associated with persistent low HBV DNA and HBs antigen clearance in patients with chronic hepatitis B.

    Science.gov (United States)

    Honda, T; Ishigami, M; Ishizu, Y; Kuzuya, T; Hayashi, K; Ishikawa, T; Murakami, Y; Iwadate, M; Umeyama, H; Toyoda, H; Kumada, T; Katano, Y; Goto, H; Hirooka, Y

    2017-06-01

    When considering treatment for chronic hepatitis B (CHB), it is important to discriminate between patients with persistent low HBV DNA and patients with active hepatitis, who may proceed to cirrhosis. In this study, we sought to identify mutations in patients expected to have persistent low HBV DNA and ultimately exhibit clearance of hepatitis B surface antigen (HBsAg). Serum samples were obtained from 33 CHB genotype C patients, divided based on HBV DNA and alanine aminotransferase (ALT) levels following observation for >2 years: Group A (n=10), transient HBV DNA ≥5.0 log copies/mL and ALT ≥120 IU/L; Group B (n=11), persistent HBV DNA DNA mutation and the additional mutation P79Q. We compared cumulative incidences of persistent low HBV DNA and HBsAg clearance in patients with or without I97L and P79Q by the Kaplan-Meier method. Incidence of Core mutation I97L differed significantly among groups: A, 30% (3/10); B, 36.4% (4/11); C, 83.3% (10/12) (p = 0.021). Cumulative incidences of persistent low HBV DNA and HBsAg clearance were significantly higher in patients with I97L than in those with wild-type I97 (p = 0.003 and p = 0.016, respectively), and even higher in those with P79Q. In patients with CHB, measurement of I97L and additional mutation P79Q would be useful for predicting persistent low HBV DNA, normal ALT, and HBsAg clearance. Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  3. Lack of evidence of viral reactivation in HBsAg-negative HBcAb-positive and HCV patients undergoing immunosuppressive therapy for psoriasis.

    Science.gov (United States)

    Morisco, Filomena; Guarino, Maria; La Bella, Serena; Di Costanzo, Luisa; Caporaso, Nicola; Ayala, Fabio; Balato, Nicola

    2014-12-19

    HBV and HCV reactivation have been widely reported in patients undergoing immunosuppressive therapy (IT); however, few data are available on the risk of reactivation in patients with psoriasis receiving IT. The aim of our study was to assess the prevalence of HBV and HCV infection in patients with psoriasis and to evaluate the effects of IT during the course of the infection. The study included psoriatic patients who attended an Italian tertiary referral hospital from 2009 to 2012. A total of 224 patients were enrolled. We evaluated: HBV and HCV markers, type of IT and the occurrence of viral reactivation. The observational period ranged from the beginning of IT to the last visit, with a mean follow-up period of 54 months. Two hundred and twenty patients (135 males and 89 females; mean age 59 years; range 18-86 years) with psoriasis, with or without psoriatic arthritis, receiving conventional IT and/or biological drugs were tested for markers of infection. We identified 23/224 patients (10.2%) with isolated positivity for HBcAb positivity, 36/224 (16%) with positivity for HBsAb/HBcAb, and 15/224 (6.6%) with positivity for HCV-Ab. No patient was HBsAg positive, none of them underwent pre-emptive therapy with lamivudine or other antiviral drugs and no one showed episodes of viral reactivation. The prevalence of HBsAg in patients with psoriasis is lower than that observed in the general population. The prevalence of isolated positivity for HBcAb and of combined positivity for HBcAb and HBsAb is 10.2% and 16%, respectively. The prevalence of HCV infection (HCV-RNA+) is 4%. In patients with psoriasis and HCV-Ab or HBcAb positivity, the IT seems to be safe, regardless of the type of drugs.

  4. Heteroassembled gold nanoparticles with sandwich-immunoassay LSPR chip format for rapid and sensitive detection of hepatitis B virus surface antigen (HBsAg).

    Science.gov (United States)

    Kim, Jinwoon; Oh, Seo Yeong; Shukla, Shruti; Hong, Seok Bok; Heo, Nam Su; Bajpai, Vivek K; Chun, Hyang Sook; Jo, Cheon-Ho; Choi, Bong Gill; Huh, Yun Suk; Han, Young-Kyu

    2018-06-01

    This study aimed to develop a more sensitive method for the detection of hepatitis B surface antigen (HBsAg) using heteroassembled gold nanoparticles (AuNPs). A single layered localized surface plasmon resonance (LSPR) chip format was developed with antigen-antibody reaction-based detection symmetry using AuNPs, which detected HBsAg at 10 pg/mL. To further improve the detection limit, a modified detection format was fabricated by fixing a secondary antibody (to form a heteroassembled sandwich format) to the AuNP monolayer, which enhanced the detection sensitivity by about 100 times. The developed heteroassembled AuNPs sandwich-immunoassay LSPR chip format was able to detect as little as 100 fg/mL of HBsAg within 10-15 min. In addition, the heteroassembled AuNPs sandwich-immunoassay LSPR chip format did not show any non-specific binding to other tested antigens, including alpha fetoprotein (AFP), C-reactive protein (CRP), and prostate-specific antigen (PSA). These findings confirm that the proposed detection strategy of heteroassembled AuNPs sandwich-immunoassay LSPR chip format may provide a new platform for early diagnosis of various human diseases. Copyright © 2018 Elsevier B.V. All rights reserved.

  5. Study of building typology of school constructed during the Dutch Colonial Period in Indonesia. Case study of Hoogere Burgerschool (HBS) in Bandung

    Science.gov (United States)

    Sarwo Wibowo, Arif

    2018-03-01

    Bandung is one of the most important colonial cities in Indonesia. In the early 20th century the capital city of Dutch East-Indies Government planned to move in Bandung. Critical infrastructures were intensively built during that period, such as streets and railways, houses, governmental buildings, train stations, hospitals and educational facilities. Besides the famous campus of Technische Hoogeschool te Bandoeng (ITB), still in the same period, several schools were also constructed. One of the most important schools was Hoogere Burgerschool in Bandung (HBS Bandung), now SMUN 3 and 5, Bandung designed by Charles Prosper Wolff Schoemaker and constructed in 1915. HBS Bandung was the fourth HBS constructed by Dutch East Indies Government, therefore became important and put itself as a reference for the later school buildings in Bandung. This study is analyzing how the architects’ frame of mind in producing this design works. Survey and direct data collecting were used to take the exact embodiment of building design. Usage and functional analysis were also used to match space and other standard used in a school building at that time. This study will give an understanding of building typology of school during the Dutch Colonial Period in Indonesia.

  6. Anticuerpos monoclonales contra la gonadotropina coriónica humana (hCG para su uso en la detección de embarazo Monoclonal antibodies against human chorionic gonadotropin (HCG for their use in pregnancy detection

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    Bertha V. Rodríguez Pendás

    2004-12-01

    Full Text Available Se reporta la generación de 2 anticuerpos monoclonales (AcM de ratón dirigidos contra la hormona gonadotropina coriónica humana (hCG, a partir de la inmunización de ratones BALB/c con hCG humana, purificada en el Instituto Nacional de Endocrinología (INEN. Los AcM obtenidos son de la clase IgG y fueron purificados a partir de líquido ascítico, mediante cromatografía de afinidad en proteína G Sepharosa. El estudio de afinidad y especificidad demostró que estos anticuerpos podían ser útiles en ensayos inmunoenzimáticos, con el uso de uno de ellos en el sistema microELISA, de nuestra institución, para la detección cualitativa de embarazo en orina.The generation of 2 mouse monoclonal antibodies directed against the human chorionic gonadotropin hormone (CGh, starting from the immunization of BALB/c mice with human CGh purified at the National Institute of Endocrinology (NIEN is reported. IgG monoclonal antibodies were obtained. They were purified starting from the ascitic fluid by affinity chromatography in protein G Sepharose. The affinity and specificity study showed that these antibodies could be useful in immunoenzimatic assays, using one of them in the microELISA system of our institution for the qualitative detection of pregnancy in urine.

  7. Correlation between HBsAg, prothrombin time activity, and indocyanine green retention rate at 15 minutes in patients with HBeAg-positive chronic HBV infection

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    FAN Wenhai

    2016-11-01

    Full Text Available ObjectiveTo investigate the correlation between HBsAg, prothrombin time activity (PTA, and indocyanine green retention rate at 15 minutes (ICG R15 in patients with HBeAg-positive chronic HBV infection. MethodsA total of 92 patients with HBeAg-positive chronic HBV infection who were admitted to The First Hospital of Lanzhou University from December 2015 to April 2016 were enrolled and divided into chronic hepatitis B (CHB group (24 patients, compensated liver cirrhosis group (38 patients, and decompensated liver cirrhosis group (30 patients. Serum HBsAg quantitation, PTA test, and liver reserve function test (ICG R15 were performed for all patients. The chi-square test was used for comparison of categorical data between groups, an analysis of variance was used for comparison of continuous data between multiple groups, and a Pearson correlation analysis was also performed. ResultsThere were significant differences between the three groups in serum HBsAg quantitation (3.82±0.43 log10IU/ml vs 2.88±0.36 log10IU/ml vs 2.60±0.27 log10IU/ml, F=25.19, P<0.001, ICG R15 (7.51%±3.10% vs 9.57%±8.18% vs 24.13%±14.28%, F=24.00, P=0.001, and PTA (8100%±1762% vs 83.08%±9.64% vs 62.32%±16.90%, F=13.42, P=0.009. The correlation analysis showed that PTA was negatively correlated with ICG R15 in all three groups (r=-0.948, -0.602, and -0.735, all P<0.01. In the compensated liver cirrhosis group and decompensated liver cirrhosis group, HBsAg was positively correlated with PTA (r=0.410 and 0.473, both P<0.05 and negatively correlated with ICG R15 (r=-0.427 and -0.768, P<0.01. ConclusionIn HBeAg positive patients, there are certain correlations between HBsAg, PTA, and ICG R15, which, to a certain degree, reflects the liver reserve function in patients with chronic HBV infection.

  8. Detection of PMTV Using Polyclonal Antibodies Raised Against a Capsid-Specific Peptide Antigen / Detección de PMTV Utilizando Anticuerpos Policlonales Contra un Péptido Antigénico Derivado de la Cápside Viral

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    Yuliana Gallo García

    2013-12-01

    Full Text Available Potato mop-top virus (PMTV; genus Pomovirus;family Virgaviridae is the causing agent of the spraing disease in potato (Solanum tuberosum. PMTV is transmitted by Spongospora subterranea f. sp. subterranea (Sss. This disease has a widespread distribution in potato growing regions around the world. The possibility of obtaining strain specific antibodies at low cost can greatly increase the sensitivity and use of serological tests in seed certification programs, plant breeding and quarantine regulations to avoid dissemination of this injurious virus. This work presents an alternative procedure for the production of PMTV specific antibodies useful in serological test such as ELISAand lateral flow. In contrast to standard methods requiring theisolation of viral particles or expression of recombinant capsid, this method uses peptides mimicking the N-terminal region of PMTV capsid protein as antigen for the production of specific polyclonal antibodies. The antibodies were tested against bait plants grown in soil infested with viruliferous Sss, as well as potato plants obtained from naturally Sss infested fields in Colombia. PMTV was detected in 9/14 and 24/28 foliage samples of N. benthamiana and S. phureja, respectively. In the case of field plants, the virus wasdetected in eight out of 12 root tissues evaluated. The minimumpeptide concentration detected by ELISA was of the order of 0.1 nM. / Potato mop-top virus (PMTV; género Pomovirus; familia Virgaviridae es transmitido por Spongospora subterranea f. sp. subterranea (Sss, agente causal de la sarna polvosa de la papa. Esta enfermedad tiene una amplia distribución en las regiones cultivadoras de papa alrededor del mundo. La posibilidad de obtener anticuerpos específicos contra cepas de este virus, puede incrementar la sensibilidad y la utilización de pruebas serológicas en programas de certificación de semilla, mejoramiento genético y regulaciones cuarentenarias que eviten su diseminaci

  9. Analysis of immunological mechanisms exerted by HBsAg-HBIG therapeutic vaccine combined with Adefovir in chronic hepatitis B patients.

    Science.gov (United States)

    Zhou, Chenliang; Li, Chaofan; Gong, Guo-Zhong; Wang, Shuang; Zhang, Ji-Ming; Xu, Dao-Zhen; Guo, Li-Min; Ren, Hong; Xu, Min; Xie, Qing; Pan, Chen; Xu, Jie; Hu, Zhongyu; Geng, Shuang; Zhou, Xian; Wang, Xianzheng; Zhou, Xiaoyu; Mi, Haili; Zhao, Gan; Yu, Wencong; Wen, Yu-Mei; Huang, Lihua; Wang, Xuan-Yi; Wang, Bin

    2017-09-02

    An HBsAg-HBIG therapeutic vaccine (Yeast-derived Immune Complexes, YIC) for chronic hepatitis B (CHB) patients has undergone a series of clinical trials. The HBeAg sero-conversion rate of YIC varied from 21.9% to 14% depending on the immunization protocols from 6 to 12 injections. To analyze the immunological mechanisms exerted by 6 injections of YIC, 44 CHB patients were separately immunized with YIC, alum as adjuvant control or normal saline as blank control, with add on of antiviral drug Adefovir in all groups. Kinetic increase in Th1 and Th2 cells CD4 + T cell sub-populations with association in decrease in Treg cells and increase of Tc1 and Tc17 cells in CD8 + T cells were observed in YIC immunized group. No such changes were found in the other groups. By multifunctional analysis of cytokine profiles, significant increase of IL-2 levels was observed, both in CD4 + and CD8 + T cells in the YIC immunized group, accompanied by increase in IFN-gamma and decrease of inhibitory factors (IL-10, TGF-β and Foxp3) in CD4 + T cells. In the alum immunized group, slight increase of IL-10, TGF-β and Foxp3 in CD4 + T cells was found after the second injection, but decreased after more injections, suggesting that alum induced early inflammatory responses to a certain extent. Similar patterns of responses of IL-17A and TNF-α in CD8 + T cells were shown between YIC and the saline group. Results indicate that add on of Adefovir, did not affect host specific immune responses.

  10. Prevalence of antibodies against Kilham virus in experimental rat colonies of Argentina Prevalencia de anticuerpos contra el virus Kilham en colonias experimentales de ratas de Argentina

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    M. P. Cagliada

    2010-02-01

    este agente, particularmente en la producción de animales de laboratorio. En este estudio se analizó la prevalencia de anticuerpos contra el virus Kilham en ratas adultas provenientes de bioterios convencionales de Argentina utilizando la prueba de inhibición de la hemoaglutinación. La seroprevalencia varió entre 27,8% y 75%. Este resultado confirma que el virus está circulando en los bioterios convencionales de Argentina, por lo que podría estar interfiriendo con las investigaciones. La infección por este virus debe ser prevenida aplicando sistemas de vigilancia y control, y manteniendo a los animales con sistemas de barrera.

  11. Detección de anticuerpos contra Trypanosoma cruzi en Somoto, Nicaragua, mediante ELISA indirecto e IFI en muestras de sangre en papel de filtro

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    Palacios Xiomara

    2000-01-01

    Full Text Available Se estandarizó un inmunoensayo enzimático en fase sólida (ELISA para estudiar la presencia de anticuerpos contra Trypanosoma cruzi en personas asintomáticas que viven en un área endémica de enfermedad de Chagas en Nicaragua. El ensayo fue estandarizado para el análisis de muestras de sangre colectadas en papel de filtro como método simple de transporte de muestras de sangre. Se realizó un estudio previo en el que se estudiaron por ELISA 18 muestras de suero total y 18 eluidos de sangre de pacientes con enfermedad de Chagas crónica, 30 muestras de suero y 30 eluidos de sangre de personas sanas que se utilizaron como controles negativos y 14 muestras de suero y 14 eluidos de sangre de pacientes con leishmaniasis cutánea o visceral que se utilizaron para los estudios de reacciones cruzadas. Tanto con el suero total como con los eluidos de sangre, la prueba de ELISA proporcionó una sensibilidad de 100% y una especificidad de 90%; solo se observaron reacciones cruzadas con las muestras de pacientes con leishmaniasis visceral. El estudio poblacional incluyó a ocho comunidades rurales de Somoto, Nicaragua. Mediante un muestreo al azar se colectaron muestras de sangre en papel de filtro a 2 434 personas (1 335 del sexo masculino y 1 099 del sexo femenino de las comunidades de Aguas Calientes, La Manzana, Los Canales, Santa Rosa, Las Playas, El Brocal, Santa Isabel y Santa Teresa. Las muestras fueron estudiadas por ELISA e inmunofluorescencia indirecta (IFI encontrándose un total de 260 seropositivos por ELISA (10,7%, 207 de los cuales fueron también positivos por IFI (8,5%. La mayoría de los sueros seropositivos correspondieron a personas del sexo femenino con ambas técnicas, pero la diferencia entre hombres y mujeres no fue estadísticamente significativa. Los resultados obtenidos con ambas técnicas mostraron una excelente concordancia. Con respecto a la edad se observó una curva ascendente, con 5,4% de seropositivos por ELISA en

  12. Study on Anti-Hepatitis B Surface Antibody Titer and Specific Interferon Gamma Response Among Dentists

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    Manoochehr Makvandi

    2017-01-01

    Full Text Available Background Hepatitis B virus (HBV is a major problem for healthcare workers worldwide, and among them, dentists are at risk of acquiring HBV infection. The prevalence of HBV infection has been reported among the dentists in different regions of the world. Since none of the available drugs can clear HBV infection, the presence of effective immunity against HBV infection is important to prevent HBV infection. Objectives This study aimed at determining HBs antibody and specific HBV gamma interferon among the dentists, who received hepatitis B vaccine. Methods The blood samples were collected from 40 dentists, including 7 endodontics, 2 oral and maxillofacial radiologist, 4 periodontics, 11 oral and maxillofacial surgeons, 6 implantologists, 3 orthodontics, 1 oral and maxillofacial pathologist, 2 esthetic and restorative dentists, and 4 doctors of dental surgery (DDS at from dental college of Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran during December, 2013. Overall, 31 (77.5% dentists had already received 3 doses of recombinant hepatitis B vaccine, and 9 (22.5% had received only two doses of the vaccine. Their sera were tested for HBsAb and anti-HBc-IgG by the Enzyme Linked Immunosorbent Assay (ELISA test. The lymphocyte of individuals was separated from their blood sample by Ficoll-Hypaque, cells were washed with phosphate buffered saline (PBS by centrifugation, and finally the pellet cells was resuspended in RPMI-1640 media. Separated cells were exposed to 2.5 µg of purified recombinant HBs antigen, and supernatants were collected after 72 hours and tested for detection of specific interferon γ level by ELISA test. Results Overall, 97.5% of dentists showed positive HBs antibody test results while 36 showed (90% positive test results for specific interferon γ against hepatitis B virus infection. Conclusions High coverage of 97.5% immune response against hepatitis B infection was found, indicating high efficacy of recombinant

  13. Investigation of occult hepatitis B virus infection in anti-hbc positive patients from a liver clinic.

    Science.gov (United States)

    Martinez, Maria Carmela; Kok, Chee Choy; Baleriola, Cristina; Robertson, Peter; Rawlinson, William D

    2015-01-01

    Occult hepatitis B infection (OBI) is manifested by presence of very low levels (Hepatitis B viral DNA (HBV DNA) in the blood and the liver while exhibiting undetectable HBV surface antigen (HBsAg). The molecular mechanisms underlying this occurrence are still not completely understood. This study investigated the prevalence of OBI in a high-risk Australian population and compared the HBV S gene sequences of our cohort with reference sequences. Serum from HBV DNA positive, HBsAg negative, and hepatitis B core antibody (anti-HBc) positive patients (study cohort) were obtained from samples tested at SEALS Serology Laboratory using the Abbott Architect, as part of screening and diagnostic testing. From a total of 228,108 samples reviewed, 1,451 patients were tested for all three OBI markers. Only 10 patients (0.69%) out of the 1,451 patients were found to fit the selection criteria for OBI. Sequence analysis of the HBV S gene from 5 suspected OBI infected patients showed increased sequence variability in the 'a' epitope of the major hydrophilic region compared to reference sequences. In addition, a total of eight consistent nucleotide substitutions resulting in seven amino acid changes were observed, and three patients had truncated S gene sequence. These mutations appeared to be stable and may result in alterations in HBsAg conformation. These may negatively impact the affinity of hepatitis B surface antibody (anti-HBs) and may explain the false negative results in serological HBV diagnosis. These changes may also enable the virus to persist in the liver by evading immune surveillance. Further studies on a bigger cohort are required to determine whether these amino acid variations have been acquired in the process of immune escape and serve as markers of OBI.

  14. Producción de la proteína de choque térmico HSC70 Recombinante en Escherichia Coli BL21 (DE3 para generar anticuerpos policlonales

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    Rocio Cely Castro

    2006-07-01

    Full Text Available Antecedentes. Los organismos vivos responden al estrés aumentando la síntesis de proteínas. El estrés por choque térmico ha sido el más estudiado y las proteínas que se inducen, se han denominado genéricamente como proteínas de choque térmico. Objetivo.En este trabajo se establecieron las condiciones óptimas de producción de la proteína HSC70, expresada en E.coli BL21 (DE3 y de los anticuerpos policlonales que permitan identificarla. Material y métodos. Mediante varios ensayos se establecieron las concentraciones óptimas del agente inductor isopropil- -D-thiogalacto­piranosido (PTG, del inóculo bacteriano, de empleo de los plásmidos de expresión pET-3a y pET-28a (+; y el método más eficiente para la recuperación de las formas soluble o insoluble de la proteína y de anticuerpos policlonales que la identifiquen. Resultados. Encontramos que el inóculo de cinco colonias con IPTG (2mM en tubos con cinco mililitros de medio modificado e incubadas por 24 horas a 37°C con agitación constante (200 r.p.m y tratamiento de sonicación, produce el mejor rendimiento de HSC70. La calidad de la proteína inducida se estableció mediante "Western blotting". Conclusión. La proteína recombinante así obtenida permitió, generar anticuerpos policlonales que a su vez permiten detectar la proteína HSC70 natural en la membrana citoplasmática de diferentes células por inmunofluorescencia, en ELISA, en Western Blot y en pruebas de bloqueo de infección de rotavirus.

  15. A meta-analysis of the antiviral activity of the HBV-specific immunotherapeutic TG1050 confirms its value over a wide range of HBsAg levels in a persistent HBV pre-clinical model.

    Science.gov (United States)

    Kratzer, Roland; Sansas, Benoît; Lélu, Karine; Evlachev, Alexei; Schmitt, Doris; Silvestre, Nathalie; Inchauspé, Geneviève; Martin, Perrine

    2018-02-01

    Pre-clinical models mimicking persistent hepatitis B virus (HBV) expression are seldom, do not capture all features of a human chronic infection and due to their complexity, are subject to variability. We report a meta-analysis of seven experiments performed with TG1050, an HBV-targeted immunotherapeutic, 1 in an HBV-persistent mouse model based on the transduction of mice by an adeno-associated virus coding for an infectious HBV genome (AAV-HBV). To mimic the clinical diversity seen in HBV chronically infected patients, AAV-HBV transduced mice displaying variable HBsAg levels were treated with TG1050. Overall mean percentages of responder mice, displaying decrease in important clinical parameters i.e. HBV-DNA (viremia) and HBsAg levels, were 52% and 51% in TG1050 treated mice, compared with 8% and 22%, respectively, in untreated mice. No significant impact of HBsAg level at baseline on response to TG1050 treatment was found. TG1050-treated mice displayed a significant shorter Time to Response (decline in viral parameters) with an Hazard Ratio (HR) of 8.3 for viremia and 2.6 for serum HBsAg. The mean predicted decrease for TG1050-treated mice was 0.5 log for viremia and 0.8 log for HBsAg, at the end of mice follow-up, compared to no decrease for viremia and 0.3 log HBsAg decrease for untreated mice. For mice receiving TG1050, a higher decline of circulating viremia and serum HBsAg level over time was detected by interaction term meta-analysis with a significant treatment effect (p = 0.002 and pHBV-persistent model mimicking clinical situations.

  16. CD205-TLR9-IL-12 axis contributes to CpG-induced oversensitive liver injury in HBsAg transgenic mice by promoting the interaction of NKT cells with Kupffer cells.

    Science.gov (United States)

    Hou, Xin; Hao, Xiaolei; Zheng, Meijuan; Xu, Congfei; Wang, Jun; Zhou, Rongbin; Tian, Zhigang

    2017-08-01

    Gut-derived bacterial products contribute to liver inflammation and injury during chronic hepatitis B virus infection; however, the underlying mechanisms remain obscure. In this study, hepatitis B surface antigen transgenic (HBs-Tg) mice and their wild-type (WT) control C57BL/6 mice were injected with CpG-oligodeoxynucleotides (ODNs) to mimic the translocation of gut microbial products into the systemic circulation. We found that, compared with the WT mice, the HBs-Tg mice were oversensitive to CpG-ODN-induced liver injury, which was dependent on natural killer T (NKT) cells. CpG-ODN injection enhanced the expression of Fas ligand (FasL) on NKT cells. In addition, hepatocytes from the HBs-Tg mice expressed higher levels of Fas than did those from the WT mice, which was further augmented by CpG-ODN. Interaction of Fas and FasL was involved in the cytotoxicity of NKT cells against hepatocytes in the HBs-Tg mice. Moreover, Kupffer cells in the HBs-Tg mice expressed higher levels of CD205 and produced greater amounts of interleukin (IL)-12 than did those in the WT mice. Finally, the depletion of Kupffer cells, neutralization of IL-12 or specific silencing of CD205 on Kupffer cells significantly inhibited CpG-ODN-induced liver injury and NKT activation in the HBs-Tg mice. Our data suggest that CD205-expressing Kupffer cells respond to CpG-ODNs and subsequently release IL-12 to promote NKT cell activation. Activated NKT cells induce liver damage through the Fas signaling pathway in HBs-Tg mice.

  17. Hepatitis B reactivation in HBsAg-negative/HBcAb-positive allogeneic haematopoietic stem cell transplant recipients: risk factors and outcome.

    Science.gov (United States)

    Mikulska, M; Nicolini, L; Signori, A; Rivoli, G; Del Bono, V; Raiola, A M; Di Grazia, C; Dominietto, A; Varaldo, R; Ghiso, A; Bacigalupo, A; Viscoli, C

    2014-10-01

    HBsAg-negative/HBcAb-positive haematopoietic stem cell transplant (HSCT) recipients are at high risk of hepatitis B virus (HBV) reactivation. Allogeneic HSCT recipients from years 2000 to 2010 were evaluated in order to study the impact of being HBsAg-negative/HBcAb-positive in this population. Overall, 137 of 764 patients (18%) were HBsAg-negative/HBcAb-positive before HSCT. Overall survival, non-relapse mortality (NRM), acute and chronic graft-vs.-host disease were similar in HBcAb-positive and HBcAb-negative patients. Reactivation occurred in 14 patients (10%) within a median of 19 months after HSCT (range 9-77). Cause-specific hazard for reactivation was decreased in the case of an HBV-immune/exposed donor (HRadjusted = 0.12; 95% CI, 0.02-0.96; p 0.045) and increased in patients who received rituximab treatment (HRadjusted = 2.91; 95%CI, 0.77-10.97; p 0.11). Competing risk analyses documented a protective role of an HBV-immune/exposed donor (p 0.041) and an increased probability associated with the length of treatment with cyclosporine (p <0.001) and treatment with rituximab (but not with low-dose rituximab prophylaxis, p <0.001 at each landmark point). No differences in overall survival and NRM were found between patients with and without HBV reactivation. The donor's immunity was independently and consistently associated with a decreased risk of HBV reactivation, while rituximab and cyclosporine treatments increased the probability. © 2014 The Authors Clinical Microbiology and Infection © 2014 European Society of Clinical Microbiology and Infectious Diseases.

  18. [Relationship between HBeAg from HBsAg positive mothers and regulatory T cells in neonates and its influence on HBV intrauterine transmission].

    Science.gov (United States)

    Hao, H Y; Yang, Z Q; Xu, X X; Wang, X F; Wang, B; Shi, X H; Fu, Z D; Wang, B; Wang, S P

    2017-10-10

    Objective: To explore the relationship between HBeAg in HBsAg positive mothers and CD(4)(+)CD(25)(+) Foxp3 (+)regulatory T cells (Treg) in newborns, as well as how they would influence the increasing risk on HBV intrauterine transmission. Methods: We collected information on general demographic characteristics and delivery on 270 HBsAg positive mothers and their newborns from the Third People's Hospital of Taiyuan. Fluorescence quantitative polymerase chain reaction (FQ-PCR) and chemiluminescence immunoassay (CLIA) were used to detect HBV DNA and HBV serological markers in peripheral blood from both mothers and neonates. The expression of Treg and other immune cells in peripheral blood of neonates were detected with flow cytometry (FCM). Results: Maternal HBeAg positive rates were associated with an increased risk of intrauterine transmission ( OR =4.08, 95 %CI : 1.89-8.82). Rates of Treg in newborns born to HBsAg-positive mothers were higher than that of the negative group ( Z =2.29, P =0.022). Each pair of the subjects was assigned to five different groups according to the HBeAg titers of mothers. Frequencies of both Treg and HBeAg in newborns and HBV DNA in mothers between the above said 5 groups showed similar trends of changing patterns and the differences between groups were statistically significant(χ(2)=18.73, P HBV DNA, mother's HBeAg titers were positively related to the percentage of Treg in their newborns ( r(s) =0.19, P =0.039). In addition, the frequencies of Treg were negatively correlated with pDC and CD(4)(+) T cell in their newborns ( r(s) =-0.21, P =0.017; r(s) =-0.23, P =0.009). Conclusion: HBeAg from HBsAg positive mothers might have inhibited the function of neonatal DC cells and T cells to reduce the immune response to HBV by up-regulating the proportion of Treg and finally increased the risk of HBV intrauterine transmission.

  19. Caracterización de IgM, IgG total, IgG1 y anticuerpos de cadena pesada en calostro de llamas (Lama Glama) mediante Elisa

    OpenAIRE

    Caggiano, Nicolás; Saccodossi, Natalia; Gentile, Maria Teresa; Chiappe Barbará, María Angelina; Leoni, Juliana; de Simone, Emilio Adrian

    2016-01-01

    Objetivos: determinar los niveles de IgM, IgG total y Anticuerpos de Cadena Pesada (HCAbs; por su sigla en inglés Heavy Chain Antibodies) (IgG2 e IgG3) en calostro de llamas y evaluar la concentración de HCAbs en relación a la IgG total y al isotipo convencional IgG1. Métodos: en este estudio se utilizaron 15 llamas preñadas, que fueron ordeñadas dentro de las primeras 24 horas post-parto. Se diseñaron ELISAs Sandwich para la cuantificación de IgM total, IgG total e IgG1. La concent...

  20. Producción y caracterización de un anticuerpo monoclonal que reconoce el fragmento 28-30 kDa de la proteína MSP-1 de Plasmodium falciparum

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    Nina M. Muñoz

    2000-06-01

    Full Text Available Los merozoitos del parásito Plasmodium falciparum contienen en su superficie los fragmentos producidos por el primer procesamiento proteolítico de la proteina MSP-1 (merozoite surface protein-1. Dichos fragmentos tienen pesos moleculares relativos aproximados de 83, 42, 38 y 28-30 kDa, respectivamente. En este estudio se describe la producción y caracterización de un anticuerpo monoclonal, denominado 8F4, que reconoce la proteina MSP-1 dadas las siguientes características del antigeno: 1 localización subcelular, 2 peso molecular relativo, 3 período de expresión durante el ciclo eritrocítrico, 4 tipo de asociación con la membrana plasmática del parásito y 5 presencia de epítopes compartidos con MSP-1. Adicionalmente. se estableció que 8F4 reconoce el fragmento de 28-30 kDa, producto del primer procesamiento proteolitico de MSP-1. Los análisis realizados permiten confirmar que MSP-1, 28-30 , no permanece en la superficie del merozoito desoués de ocurrida la invasión. lo cual suoiere oue hace oarte del com.o le.io de polipéptidos que es liberado por el merozoito previamente al evento de la invasión. 8F4 es el primer anticuerpo monoclonal reportado hasta el momento que reconoce específicamente este fragmento.

  1. Diagnóstico rápido de citomegalovirus (CMV en pacientes inmunocomprometidos mediante anticuerpos monoclonales que reconocen proteinas precoces virales Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by using monoclonal antibodies against early viral antigens

    Directory of Open Access Journals (Sweden)

    Maritza Alvarez

    1989-06-01

    Full Text Available Se aplicó la técnica de detección de antigenos precoces fluorescentes (DAPF usando el anticuerpo monoclonal E-13 McAb, mediante el cual se lograron detectar 15 casos positivos a CMV de 75 muestras de orina o sangre ("buffy coat" tomadas de 52 pacientes inmunocomprometidos ingresados en el Instituto de Nefrología de ciudad Habana. Aplicando las técnicas clásicas de aislamiento en fibroblastos humanos diploides (MRC-5, se lograron aislar 12 cepas de CMV de casos previamente positivos por DAPF; lográndose además un aislamiento en una muestra reportada negativa por fluorescencia. Se observó una coincidencia de un 80% entre ambas técnicas. Se detectó la presencia de anticuerpos IgG contra CMV en todos los casos estudiados, utilizando para ello la técnica ELISA.A technique was applied to detect early fluorescent antigens (DEFA of cytomegalovirus (CMV using the E13 monoclonal antibodies in 52 immunocompromised patients hospitalized in the Nephrology Institute of Havana. Of the 75 urine or blood (buffy coat samples taken, 15 were found positive to CMV. Using classical diploide human fibroblast isolation technique, 12 CMV strains were isloation of previously detected positive samples by DEFA. In addition, CMV was isolated from one sample reported to be negative by DEFA. A coincidence of 80% was found between both techniques. With the ELISA test, all the sample studied have IgG antibodies to CMV.

  2. Determinación de anticuerpos contra patógenos virales y bacterianos seleccionados en la población de cerdos silvestres (Sus scrofa de la Reserva Natural Bahía Samborombón, Argentina

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    B. Carpinetti

    2017-06-01

    Full Text Available Los cerdos silvestres (Sus scrofa descienden de cruzamientos entre cerdos domésticos liberados durante la colonización con jabalíes salvajes euroasiáticos, liberados con propósitos cinegéticos. Son invasivos y su coexistencia con especies domésticas implica riesgos sanitarios. Argentina es considerada libre de fiebre aftosa (FA, peste porcina clásica (PPC y africana (PPA y síndrome reproductivo y respiratorio porcino (PRRS. La enfermedad de Aujeszky (EA y la leptospirosis son endémicas en ciertas áreas del país. El objetivo fue evaluar la presencia de ciertas enfermedades zoonóticas y/o de importancia para la producción animal y la conservación de la biodiversidad en cerdos silvestres de la Bahía de Samborombón. Se capturaron 118 animales. Se tomaron muestras de suero, tonsilas, músculo, intestino delgado, linfonódulos, entre otras. Se estudió la presencia de anticuerpos contra Brucella spp., coronavirus respiratorio porcino, virus de la estomatitis vesicular, de la FA, de la gastroenteritis transmisible porcina (TGEV, de la PPC, PPA, EA, PRRS y Leptospira spp. Se realizaron análisis bacteriológicos para Mycobacterium spp. Los resultados ratificaron la ausencia de las enfermedades exóticas e indicaron que 36 % de los animales presentó anticuerpos contra Leptospira interrogans serovar pomona y 62,5 % contra el virus de la EA. Estos resultados remarcan la importancia del monitoreo de la interfase productiva/silvestre en función de la salud pública, producción animal y conservación de la biodiversidad.

  3. Perfil infectológico de poblaciones mapuche de Cerro Policía y Aguada Guzmán de la provincia de Río Negro

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    Rey, Jorge Alberto

    1996-01-01

    Full Text Available Nuestro trabajo analiza la prevalencia de marcadores de algunas infecciones que afectan las poblaciones mapuche de Cerro Policía (CP y Aguada Guzmán (AG en la Prov. de Río Negro. Los estudios incluídos son: determinación de anticuerpos anti-Treponema pallidum, anti-Trypanosoma cruzii, anti-Echinococcus granulosus, antígeno de superficie de la Hepatitis B (HBsAg, anticuerpos contra el core de la Hepatitis B (anti-HBc, anticuerpos contra el virus de la Hepatitis A (anti-HAV. Se estudiaron 160 muestras en CP y 83 en AG. Los resultados muestran alta prevalencia para anti-HAV en CP (89.2% y en AG (77.1% y para hidatidosis (10.6% y 9.6% respectivamente. Se hallaron valores nulos o bajos para sífilis (CP: 0.0 % y AG:2.4%. La prevalencia para Chagas fue del 10% en CP y 0.0% en AG. Los datos para Hepatitis B fueron nulos o bajos: HBsAg (CP:0.0% y AG:1.2% y anti-HBc(CP:0.6% y AG:0.0%. Los resultados indican que, aún estando estas dos poblaciones tan próximas muestran diferencias en los perfiles de los marcadores serológicos estudiados. Al comparar las tasas de prevalencia con las de otras poblaciones de América, cada una presenta un perfil diferente que depende de la población y del agente infeccioso considerado.

  4. FRECUENCIA DE ANTICUERPOS CONTRA Neospora caninum Y Toxoplasma gondii EN CANES CON SIGNOS CLÍNICOS DE AFECCIÓN NEUROMUSCULAR.

    OpenAIRE

    Ruíz R., Nelson; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima-Perú.; Casas A., Eva; Laboratorio de Microbiología y Parasitología Veterinaria; Suárez A., Francisco; Laboratorio de Medicina Veterinaria Preventiva, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima-Perú.; Díaz C., Diego; Clínica de Animales Menores, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Fernández P., Viviana; Clínica de Animales Menores, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

    2012-01-01

    Toxoplasma gondii and Neospora caninum can cause neuromuscular disorders in dogs. The aim of the Case-Control study was to determine the association between antibodies anti N. caninum and anti-T. gondii in dogs with neuromuscular disorders. Blood samples were collected in 96 and 120 dogs with and without clinical signs of neuromuscular disorders respectively. Serum samples were analyzed by the indirect immunofluorescence test (IFI) for the detection of IgG antibodies, considering positive the...

  5. Prevalencia de anticuerpos IgG específicos dirigidos a Mycobacterium avium en caprinos y ovinos en el sur de la Guajira y norte del Cesar

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    María Fernanda Cepeda

    2016-06-01

    enviados para su procesamiento al Laboratorio de Investigaciones Biomédicas y Biotecnológicas (LIBB del programa de Bacteriología y Laboratorio Clínico de la Universidad de Santander UDES Bucaramanga. Los resultados del procesamiento de las pruebas de ELISA serán ingresados en una base de datos proporcionada por la institución responsable del control de calidad de pruebas, Laboratorios VECOL, para su posterior análisis. El análisis estadístico será realizado mediante la aplicación del paquete estadístico Graphpad Prism (La Jolla, CA, USA. Resultados: Como resultado de este trabajo se plantea la determinación de la prevalencia de anticuerpos específicos de tipo IgG dirigidos a M. avium en caprinos y ovinos, información que aportará herramientas encaminadas a la construcción de un perfil sanitario en los municipios del sur de la Guajira y el Norte del Cesar. Conclusiones: El conocimiento de la prevalencia de M. avium en ovinos y caprinos de la región del sur de la Guajira y el norte del Cesar, se concibe como un aspecto de importancia epidemiológica al ser considerada una infección de notificación obligatoria según la Organización Mundial de Sanidad Animal (OIE según su sigla en inglés dado que estos departamentos encabezan el listado de las regiones con mayor actividad económica basada en la producción y consumo de estos animales y sus productos, adicionalmente, los ovinos y caprinos tienen la capacidad de ser reservorios para este agente infeccioso, llegando a convertirse en potencial causa del desarrollo de enfermedades en estas poblaciones específicas, así como, la importancia que pueden representar desde el punto de vista de su potencial zoonótico.

  6. Genetic Variants at BCL11A and HBS1L-MYB loci Influence Hb F Levels in Chinese Zhuang β-Thalassemia Intermedia Patients.

    Science.gov (United States)

    Lai, Yunli; Chen, Yun; Chen, Biyan; Zheng, Haiyang; Yi, Sheng; Li, Guojian; Wei, Hongwei; He, Sheng; Zheng, Chenguang

    2016-11-01

    Increased Hb F levels can ameliorate the symptoms of β-thalassemia (β-thal). Due to the genetic heterogenicity of β-thal, the relationship between genetic variants in modifier genes and Hb F level has been studied in different populations. The Chinese Zhuang has the second largest population in China and has 6.78% prevalence of β-thal. However, the effects of these single nucleotide polymorphism (SNP) variants on the Hb F levels of β-thal intermedia (β-TI) patients in this population have not been reported. To explore the association between modifier loci (β-globin gene cluster, HBS1L-MYB intergenic region and BCL11A) and Hb F levels in Chinese Zhuang β-TI patients, 96 unrelated β-TI patients (50 males and 46 females) with different Hb F levels were recruited and genotyped by mass spectrometry. A total of 13 SNPs were confirmed to be in a significant relationship with Hb F levels in this population. Of these, high-risk genotypes of six Hb F-associated SNPs, rs9376090, rs7776054, rs9399137, rs9389268, rs9402685 in the HBS1L-MYB intergenic region and rs189984760 in the BCL11A locus, showed association with high Hb F levels, especially for SNPs in linkage disequilibrium. One novel Hb F-associated SNP, rs189984760, was identified in our study. Our findings will be of valuable reference for correlation between modifier genes and Hb F in Chinese Zhuang populations and may lead to better understand the modifying mechanisms for β-thal.

  7. MECOM, HBS1L-MYB, THRB-RARB, JAK2, and TERT polymorphisms defining the genetic predisposition to myeloproliferative neoplasms: A study on 939 patients.

    Science.gov (United States)

    Trifa, Adrian P; Bănescu, Claudia; Bojan, Anca S; Voina, Cristian M; Popa, Ștefana; Vișan, Simona; Ciubean, Alina D; Tripon, Florin; Dima, Delia; Popov, Viola M; Vesa, Ștefan C; Andreescu, Mihaela; Török-Vistai, Tünde; Mihăilă, Romeo G; Berbec, Nicoleta; Macarie, Ioan; Coliţă, Andrei; Iordache, Maria; Cătană, Alina C; Farcaș, Marius F; Tomuleasa, Ciprian; Vasile, Kinga; Truică, Cristina; Todincă, Adriana; Pop-Muntean, Lavinia; Manolache, Raluca; Bumbea, Horia; Vlădăreanu, Ana-Maria; Gaman, Mihaela; Ciufu, Cristina M; Popp, Radu A

    2018-01-01

    Polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF) are classical myeloproliferative neoplasms (MPN), characterized by specific somatic mutations in JAK2, CALR or MPL genes. JAK2 46/1 and TERT rs2736100 polymorphisms are known to significantly predispose to MPN. This study aimed to establish the additional contribution of the recently described MECOM rs2201862, HBS1L-MYB rs9376092 and THRB-RARB rs4858647 polymorphisms to the occurrence of MPN. These three polymorphisms, along with JAK2 46/1 and TERT rs2736100 were genotyped in 939 MPN patients (454 with ET, 337 with PV and 148 with PMF) and 483 controls. MECOM rs2201862 associated significantly with each MPN entity, except for ET, and with all major molecular sub-types, especially those CALR-mutated (OR = 1.4; 95% CI = 1.1-1.8; P-value = .005). HBS1L-MYB rs9376092 associated only with JAK2 V617F-mutated ET (OR = 1.4; 95% CI = 1.1-1.7; P-value = .003). THRB-RARB rs4858647 had a weak association with PMF only (OR = 1.5; 95% CI = 1-2.1; P-value = .04). Surprisingly, JAK2 46/1 haplotype was associated significantly not only with JAK2 V617F-mutated MPN, but also with CALR-mutated MPN (OR = 1.4; 95% CI = 1.1-1.8; P-value = .01). TERT rs2736100 was associated equally strong with all MPN, regardless of phenotype or molecular sub-type. In conclusion, JAK2 46/1, TERT rs2736100 and MECOM rs2201862 are the chief predisposing polymorphisms to MPN. © 2017 Wiley Periodicals, Inc.

  8. Antimalaric antiobodies in school children in El Bagre, Colombia Detección de anticuerpos antimaláricos en un grupo de escolares del municipio de El Bagre, Antioquia

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    Isabel Polanco

    1998-01-01

    Full Text Available A cases and controls study was carried out in 1996 in El Bagre, Colombia, endemic zone for malaria, in order to determine the relationship between nutritional situation and immune response to malaria in 51 children with malaria and 49 without it; IgG and IgM antibodies were determined by means of indirect inmunofluorescence .against P. falciparum antigens (Strain FCB2. Nutritional situation was evaluated according to USA National Center for Health Statistics. No child was found positive for IGM antibodies; concerning IgG response, it was positive in 29 (56.9% malaric children but only in 4 (8.2% of the non-malaric ones (p<0.00001 . Difference was also signjficant (p<0.00001 for malarja antecedents in the previous year (70.6% in malaric children vs 10.2% jn the nonmalaric ones. Of the 29 malaric seropositive children 20 (69% were malnourished (p<0.01 . En 1996, en el municipio de El Bagre (Antioquia-Colombia, zona endémica para malaria y con altos niveles de desnutrición, se realizó un estudio de casos y controles con el objeto de determinar la relación entre el estado nutricional y la respuesta inmune humoral de niños con y sin malaria. Se trabajó con un grupo de 100 niños entre 4 y 9 años de edad, 51 con malaria y 49 sin ella, al cual se le determinaron los niveles de anticuerpos antimaláricos IgG e IgM por la técnica de inmunofluorescencia indirecta (IFI, empleando antigenos de P. falciparum cepa FCB 2 mantenida en cultivo continuo. El estado nutricional se evaluó por comparación de las medidas antropométricas con la referencia de crecimiento del National Center for Health Statistics (NCHS de los Estados Unidos. Los resultados mostraron diferencias en la tasa de positividad de anticuerpos IgG entre los niños maláricos y los no maláricos. En efecto: Veintinueve niños maláricos (56.9% y sólo 4 de los no maláricos (8.2% tenían dichos anticuerpos (P<0.00001 ; la diferencia también fue significativa para el antecedente de

  9. Detection of hepatitis B virus DNA in sera from 18 alcoholic carriers of "anti-HBc alone" and response to a single dose of hepatitis B vaccine Pesquisa de DNA do vírus da hepatite B no soro de 18 alcoolistas portadores de "anti-HBc isolado" e resposta a uma dose da vacina para hepatite B

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    Luiz Carlos Marques de Oliveira

    2008-09-01

    Full Text Available To evaluate the possibility of occult hepatitis B virus (HBV infection in alcoholics carriers of "anti-HBc alone", and to verify the behavior of this serological pattern after a single dose of hepatitis B vaccine, 18 alcoholics who had this serological profile were evaluated by the polymerase chain reaction method, and 17 of them were vaccined. All were negative for HBV DNA. Nine (52.9% of those vaccined had anamnestic response, mainly those with positive anti-HBe (8/10; 80%. "Anti-HBc alone" was compatible with low levels of anti-HBs in half of the patients, and probably with false positive results for anti-HBc in the others.Para avaliar a possibilidade de infecção oculta pelo vírus da hepatite B em alcoolistas com "anti-HBc isolado" e a resposta a uma dose da vacina para a hepatite B, 18 alcoolistas com este perfil sorológico foram avaliados pelo método de reação em cadeia da polimerase e 17 deles foram vacinados. Todos tiveram negativos os exames para o VHB DNA. Nove (52,9% dos vacinados tiveram resposta anamnéstica, principalmente aqueles com anti-HBe positivo (8/10; 80%. "Anti-HBc isolado" foi compatível com baixos títulos de anti-HBs em metade dos pacientes, e provavelmente com resultado falso-positivo para o anti-HBc nos demais.

  10. Assessing risk of liver enzyme elevation in patients with immune-mediated diseases and different hepatitis B virus serostatus receiving anti-TNF agents: a nested case-control study.

    Science.gov (United States)

    Chiu, Ying-Ming; Lai, Mei-Shu; Chan, K Arnold

    2017-11-01

    Liver enzyme elevation is an important and common adverse effect among patients with immune-mediated diseases who receive tumour necrosis factor inhibitors (anti-TNF), and has various causes. Hence, we evaluated the relative risks of developing liver enzyme elevation in anti-TNF users with differing hepatitis B virus (HBV) infection status. At a hospital in central Taiwan, 407 patients with rheumatoid arthritis, ankylosing spondylitis, or psoriasis/psoriatic arthritis received anti-TNF therapy between 1 January 2004 and 30 June 2012. We performed a nested case-control study (n = 368) of cases with serum alanine aminotransferase (ALT) > 40 international units/L ≤ 12 months after starting anti-TNF therapy, and corresponding controls without liver enzyme elevation. Conditional logistic regression was used to evaluate associations between liver enzyme elevation and HBV serostatus, as well as other risk factors. Thirty cases were compared to 338 controls. After adjustment for potential confounders, HBV surface antigen-positive (HBsAg + ) serostatus was associated with substantially higher likelihood of developing elevated ALT (adjusted odds ratio 7.91, 95% confidence interval (CI) 2.16-31.31) relative to those with an uninfected HBV status; no such association was observed among HBsAg-negative/HBV core antibody-positive (HBsAg - /HBcAb + ) patients (adjusted odds ratio 1.00, 95% CI 0.33-3.25). Increased risk of ALT elevation was associated with methotrexate used alone, without folic acid (adjusted odds ratio 11.60, 95% CI 2.52-56.46), and history of ALT elevation (adjusted odds ratio 13.71, 95% CI 4.32-45.75). HBsAg + patients with immune-mediated diseases who received anti-TNF therapy had an approximately eight-fold higher likelihood of liver enzyme elevation than those without HBV infection, whereas patients with HBsAg - /HBcAb + serostatus had a risk similar to that of uninfected patients.

  11. Occult HBV among Anti-HBc Alone: Mutation Analysis of an HBV Surface Gene and Pre-S Gene.

    Science.gov (United States)

    Kim, Myeong Hee; Kang, So Young; Lee, Woo In

    2017-05-01

    The aim of this study is to investigate the molecular characteristics of occult hepatitis B virus (HBV) infection in 'anti-HBc alone' subjects. Twenty-four patients with 'anti-HBc alone' and 20 control patients diagnosed with HBV were analyzed regarding S and pre-S gene mutations. All specimens were analyzed for HBs Ag, anti-HBc, and anti-HBs. For specimens with an anti-HBc alone, quantitative analysis of HBV DNA, as well as sequencing and mutation analysis of S and pre-S genes, were performed. A total 24 were analyzed for the S gene, and 14 were analyzed for the pre-S gene through sequencing. A total of 20 control patients were analyzed for S and pre-S gene simultaneously. Nineteen point mutations of the major hydrophilic region were found in six of 24 patients. Among them, three mutations, S114T, P127S/T, M133T, were detected in common. Only one mutation was found in five subjects of the control group; this mutation was not found in the occult HBV infection group, however. Pre-S mutations were detected in 10 patients, and mutations of site aa58-aa100 were detected in 9 patients. A mutation on D114E was simultaneously detected. Although five mutations from the control group were found at the same location (aa58-aa100), no mutations of occult HBV infection were detected. The prevalence of occult HBV infection is not low among 'anti-HBc alone' subjects. Variable mutations in the S gene and pre-S gene were associated with the occurrence of occult HBV infection. Further larger scale studies are required to determine the significance of newly detected mutations. © Copyright: Yonsei University College of Medicine 2017

  12. DESARROLLO DE TECNOLOGIAS DE GENERACION DE ANTICUERPOS MONOCLONALES COMPLETAMENTE HUMANOS: PRODUCCION DE UN PROTOTIPO CONTRA RECEPTORES TIROSINA QUINASA ERBB PARA USO DE TERAPIA SELECTIVA EN CANCER GASTRICO

    OpenAIRE

    MOLINA SAMPAYO, MARIA CARMEN

    2011-01-01

    Actualmente, estamos siendo testigos del surgimiento de nuevas terapias anti-tumorales dirigidas contra moléculas expresadas diferencialmente en las células neoplásicas. En este sentido, algunos miembros de la familia del factor de crecimiento epidérmico ErbB (familia ErbB) han demostrado ser un buen blanco para el tratamiento de algunas neoplasias, al ser potentes mediadores del desarrollo y crecimiento celular normal. El bloqueo de la activación de los miembros de la familia Erb...

  13. Low incidence of hepatitis B virus reactivation during chemotherapy among diffuse large B-cell lymphoma patients who are HBsAg-negative/ HBcAb-positive: a multicenter retrospective study.

    Science.gov (United States)

    Ji, Dongmei; Cao, Junning; Hong, Xiaonan; Li, Junmin; Wang, Jianmin; Chen, Fangyuan; Wang, Chun; Zou, Shanhua

    2010-09-01

    Reactivation of hepatitis B virus (HBV) is less common in lymphoma patients with prior resolved HBV infection [characterized by hepatitis B surface antigen (HBsAg)-negative/hepatitis B core antibody (HBcAb)-positive status] compared with chronic HBV infection (HBsAg positive) when receiving chemotherapy alone. The use of rituximab in chemotherapy regimen might increase the risk of HBV reactivation in patients with prior resolved HBV infection. However, the incidence of HBV reactivation is uncertain, and prophylactic antiviral treatment for this group of patients during rituximab-containing chemotherapy is controversial. The objective of this study was to determine the incidence of HBV reactivation in HBsAg-negative/HBcAb-positive patients diagnosed of diffuse large B-cell lymphoma (DLBCL) and treated with CHOP-like or RCHOP-like regimen. In addition, this study also aims to explore the relationship of HBV reactivation and HBV serology. Patients were identified using data from six university hospitals collected between January 1998 and November 2008. Four hundred and thirty-seven patients with complete data were selected based on the diagnosis of CD20+ DLBCL, availability of HBV serum markers prior to initiation of chemotherapy and during the development of hepatitis, completion of at least four cycles of chemotherapy using CHOP-like or RCHOP-like regimen, and follow-up for at least 6 months after completion of treatment. The characteristics of the HBsAg-negative/HBcAb-positive patients treated with CHOP-like regimen were compared to those treated with RCHOP-like regimen. Eighty-eight patients of the total 437 patients had pretreatment serology of prior resolved hepatitis B, with a prevalence of 20.1%. Among them, 45 patients received CHOP-like regimen while 43 patients received RCHOP-like regimen. Five patients developed hepatitis during treatment, two from CHOP group and three from RCHOP group. Only one patient treated with RCHOP had hepatitis associated with HBV

  14. After anti-racism?

    OpenAIRE

    2008-01-01

    Abstract Anti-racism as a political discourse and a form of collective social action has long been ignored as a serious field of research. In contrast, I envision the study of anti-racism as a vital lens on both 'race' and racism. First, the heterogeneity of anti-racism is demonstrated, spanning both pro- and anti-state-based analyses of the origins of racism. Second, a parallel discourse of 'anti-anti-racism' within the radical Left reveals the reluctance of many on the Left to id...

  15. Anti-hepatitis B virus activity of Boehmeria nivea leaf extracts in human HepG2.2.15 cells

    Science.gov (United States)

    WEI, JINGCHEN; LIN, LIANKU; SU, XIAOJIAN; QIN, SHAOYAN; XU, QING; TANG, ZUNIAN; DENG, YAN; ZHOU, YUEHAN; HE, SONGQING

    2014-01-01

    Boehmeria nivea (Linn.) Gaudich of the Urticaceae family is a perennial ratoon herbal plant, the root of which is used in traditional Chinese medicine and possesses a variety of pharmacological properties. The 20% ethanol Boehmeria nivea root extract was shown to exert an anti-hepatitis B virus (HBV) effect in vitro and in vivo; however, whether the Boehmeria nivea leaf (BNL) extract possesses similar properties has not been determined. In this study, we aimed to investigate the anti-HBV effects of the BNL extract in HepG2.2.15 cells transfected with human HBV DNA. Our results demonstrated that the secretion of HBsAg and HBeAg was reduced in HepG2.2.15 cells treated with the BNL extract, without any recorded cytotoxic effects. In addition, the chloroform fraction (CF) and ethyl acetate fraction (EAF) of BNL were shown to be more potent compared to the other fractions: CF (100 mg/l) inhibited the secretion of HBsAg by 94.00±1.78% [inhibitory concentration 50 (IC50) = 20.92 mg/l] and that of HBeAg by 100.19±0.35% (IC50=19.67 mg/l) after 9 days of treatment. Similarly, EAF (200 mg/l) inhibited the secretion of HBsAg by 89.95±2.26% (IC50=39.90 mg/l) and that of HBeAg by 98.90±1.42% (IC50=36.45 mg/l). Furthermore, we observed that the content of HBV DNA in the medium secreted by the HepG2.2.15 cells was significantly decreased under CF (100 mg/l) or EAF (200 mg/l) treatment. Thus, we concluded that the BNL extracts exhibited anti-HBV activity, with CF and EAF being the most potent among the fractions. PMID:24649087

  16. Human polyclonal anti-hepatitis B surface antigen immunoglobulin reduces the frequency of acute rejection after liver transplantation for chronic hepatitis B A imunoglobulina policlonal humana anti-antígeno de superfície da hepatite B reduz a freqüência da rejeição aguda após transplante de fígado

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    Claudia Alves COUTO

    2001-12-01

    Full Text Available BACKGROUND: Use of polyclonal anti-hepatitis B surface antigen immunoglobulin (HBIg has been shown to reduce hepatitis B virus (HBV recurrence after liver transplantation (LT and to decrease the frequency of acute cellular rejection (ACR. However, the protective role of HBIg against ACR remains controversial, since HBV infection has been also associated with a lower incidence of ACR. AIM: To assess the relationship between HBIg immunoprophylaxis and the incidence of rejection after LT. METHODS: 260 patients (158 males, 43 ± 14 years old submitted to LT were retrospectively evaluated and divided into three groups, according to the presence of HBsAg and the use of HBIg. Group I was comprised of HBsAg-positive patients (n = 12 that received HBIg for more than 6 months. Group II was comprised of HBsAg-positive patients that historically have not received HBIg or have been treated irregularly for less than 3 months (n = 10. Group III was composed of 238 HBsAg-negative subjects that have not received HBIg. RESULTS: HBIg-treated patients (group I had significantly less ACR episodes, when compared to group II and III. No differences between groups II and III were observed. CONCLUSIONS: Long-term HBIg administration contributes independently to reduce the number of ACR episodes after LT.INTRODUÇÃO: O emprego da imunoglobulina policlonal anti-antígeno de superfície da hepatite B (HBIg tem reduzido a recorrência da hepatite B após transplante hepático (TH, assim como também a freqüência de rejeição celular aguda (RCA. No entanto, o papel protetor da HBIg contra a RCA permanece controverso, pois a própria infecção por vírus B foi também associada a menor incidência de RCA. OBJETIVOS: Verificar a relação entre HBIg e a freqüência de RCA após TH. MÉTODOS: 260 pacientes (158 do sexo masculino, com 43 ± 14 anos submetidos a TH foram avaliados, retrospectivamente, e divididos em três grupos de acordo com a presença de AgHBs e uso de

  17. Seroprevalencia de hepatitis viral B en estudiantes universitarios en Abancay, Perú Seroprevalence of viral hepatitis B in university students in Abancay, Perú

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    Max Carlos Ramírez-Soto

    2011-09-01

    Full Text Available Para determinar la prevalencia de marcadores serológicos de hepatitis viral B en estudiantes universitarios de la ciudad de Abancay, realizamos un estudio transversal en 240 estudiantes de tres universidades, entre enero a octubre de 2010. Previo consentimiento informado, se llenó, por cada estudiante, una ficha epidemiológica y se tomó una muestra sanguínea para determinar la presencia de HBsAg, anti-HBcAg total, anti-HBe, HBeAg e IgM anti-HBc por el método de ELISA. Se encontró una prevalencia de 2,5 % (seis seropositivos para el HBsAg y 28,3 % (68 seropositivos para los anticuerpos Anti-HBcAg. El sexo masculino estuvo asociado con la presencia del anti-HBcAg (OR = 2,0; IC 95 %, 1,2- 3,6. No se encontró la presencia del HBeAg e IgM anti-HBc; los seis portadores del HBsAg fueron anti-HBe positivos. En conclusión, la infección por hepatitis B sigue siendo un problema de salud pública en Abancay, con una prevalencia importante en estudiantes universitarios.To determine the prevalence of serological markers of viral hepatitis B in university students of the city of Abancay, we performed a cross-sectional study on 240 students from three universities, from January to October 2010. Informed consent was requested to every student, an epidemiological record was filled, and a venous blood sample was drawn to determine the presence of HBsAg, total anti - HBcAg, anti - HBe, HBeAg and IgM Anti - HBc by ELISA. A prevalence of 2.5% (six positive samples was found for HBsAg and of 28.3% (68 positive samples for anti - HbcAg antibodies. The male sex was associated with the presence of anti - HBcAg (OR = 2.0, 95% CI, 1.2 to 3.6. We did not found HBeAg or IgM anti - HBc, however, the 6 HBsAg carriers were anti - HBe positive. In conclusion hepatitis B infection is still a public health problem in Abancay, with a significant prevalence in university students

  18. Seroprevalence of dengue virus antibodies in asymptomatic Costa Rican children, 2002-2003: a pilot study La seroprevalencia de anticuerpos contra el virus del dengue en niños costarricenses asintomáticos, 2002-2003: estudio piloto

    Directory of Open Access Journals (Sweden)

    Roberto Iturrino-Monge

    2006-07-01

    Full Text Available OBJECTIVES: Since 1993 dengue has become more frequent in Costa Rica. Adults have been the most affected population, while children have remained virtually unharmed. So far no studies have investigated how many asymptomatic children have been affected by this virus. This pilot study documents the seroprevalence, measured as the presence of IgG antibodies, of dengue virus in asymptomatic children from two different geographical areas. METHODS: This descriptive, prospective epidemiologic study compared the presence of antibodies in children who live in a coastal region of a tropical country where dengue is endemic, and an inland area where dengue is not endemic. An enzyme-linked immunosorbent assay was used to test the serum for dengue virus IgG antibodies. None of the children had a prior history of dengue, fever, immunosuppressive therapy or underlying disease. RESULTS: During the period from July 2002 to July 2003, 103 children were recruited from each area. In the costal region we found a seroprevalence of 36.9%. In the inland area seroprevalence was 2.9% CONCLUSIONS: We found a substantial number of asymptomatic infections in Costa Rican children. This greatly increases the risk of dengue hemorrhagic fever or dengue shock syndrome in these children, in whom previous dengue infection had gone undetected. Preventive efforts should be targeted at the costal region due to the higher prevalence in this area.OBJETIVOS: Desde 1993, la frecuencia de dengue en Costa Rica ha venido aumentando. La población de adultos ha sido la más afectada, mientras que en los niños apenas se han presentado casos. Hasta el momento no se han realizado estudios para determinar cuántos niños asintomáticos se han visto afectados por el virus de la enfermedad. Este estudio piloto documenta la seroprevalencia de anticuerpos de tipo IgG contra el virus del dengue en niños asintomáticos procedentes de dos zonas geográficas distintas. MÉTODOS: En este estudio

  19. El ácido siálico N-acetilado es inmunogénico e induce anticuerpos protectores contra Neisseria meningitidis

    Directory of Open Access Journals (Sweden)

    Gregory R. Moe

    2009-08-01

    Full Text Available Recently, we showed that monoclonal antibodies (mAbs that are reactive with derivatives of polysialic acid containing de-N-acetylated neuraminic acid (Neu residues are protective against N. meningitidis group B strains (Moe et al. 2005, Infect Immun 73:2123; Flitter et al., in preparation. In addition, we found that fully de-N-acetylated PSA (i.e. poly alpha 2,8 Neu conjugated to tetanus toxoid (DeNAc elicits IgM and IgG antibodies of all subclasses in mice that bind to group B strains, activate human complement deposition, are protective in an infant rat model of meningococcal bacteremia and are bactericidal against group C strains (Moe et al, in press. We show here that anti-DeNAc mAbs, DA1 and DA2 (both IgM, are reactive with polysaccharides containing Neu, bind to group B, C, W135 and Y but not X strains grown in chemically defined media (CDM. However, when the group X strain is grown in CDM supplemented with human plasma, DA2 binds. Also both mAbs mediate bactericidal activity against B, C, W135, and X strains with human complement. The esults suggests that N. meningitidis express and/or acquire zwitterionic de-N-acetyl sialic acid antigens that can be the target of protective antibodies.

  20. Simultaneous expression of growth hormone releasing hormone (GHRH) and hepatitis B surface antigen/somatostatin (HBsAg/SS) fusion genes in a construct in the skeletal muscle enhances rabbit weight gain.

    Science.gov (United States)

    Dai, Jian-wei; Liu, Song-cai; Hao, Lin-lin; Zhang, Yong-liang; Zhang, Qianqian; Ren, Xiao-hui; Jiang, Qing-yan

    2008-01-01

    Somatostatin (SS) and growth hormone-releasing hormone (GHRH) are synthesized and secreted by the hypothalamus, which can control the synthesis and secretion of the growth hormone (GH) from the hypophysis as well as regulate the GH concentrations in animals and humans. In this article, we describe the regulation of animal growth using plasmid DNA encoding both the GHRH gene and the SS gene fused with the hepatitis B surface antigen (HBsAg) gene. We constructed a series of expression plasmids to express the GHRH and HBsAg-SS fusion genes individually as well as collectively. The fusion gene and GHRH were successfully expressed in Chinese hamster ovary (CHO) cells, as proven by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting tests. Poly D, L-lactide-co-glycolic acid (PLGA) plasmid-encapsulating microspheres were prepared and injected intramuscularly into the leg skeletal muscles of rabbits. Weight gain/day and the levels of insulinlike growth factor-I (IGF-I), SS, and hepatitis B surface antibody (HBsAb) were monitored. During days 30 postinjection, increase in weight gain/day and IGF- I concentration and decrease in SS were observed in treatment groups. From days 15 to 30 postinjection, the weight gain/day significantly increased (P gain/day (P gain/day in rabbits.

  1. Determinación de anticuerpos de leptospira spp. en pequeños mamíferos no voladores, en un fragmento de bosque andino en la montaña del Zoológico Jaime Duque

    Directory of Open Access Journals (Sweden)

    Laura V. Rojas Arbeláez

    2008-12-01

    Full Text Available Este estudio se concentró en la búsqueda y determinación de anticuerpos de los serovares L. gryppotiphosa, L. icterohaemorrhagiae, L. pomona, L. bratislava, L. hardjo y L. canicola, en pequeños mamíferos no voladores capturados por medio de trampas tipo Sherman y Tomahawk. Se utilizó la metodología de transectos lineales fijos en un área de 107.800 m2 y el empleo de cebos alimenticios como atrayentes para estas especies; en ocho meses, se capturaron 23 individuos de 7 especies diferentes, que fueron muestreados para la obtención del suero sanguíneo. Este último fue analizado en el laboratorio a través de la técnica de Microaglutinación en placa (MAT. Entre los resultados obtenidos, se destaca que en 16 individuos se encontró evidencias de la presencia de leptospira que, en términos de prevalencia puntual, equivale a 69,56% (16/23. Además, todos los serovares mostraron positividad para la mayoría de la población objetivo: para el serovar L. hardjo, 2 fueron positivos; para el L. pomona, 4; para el L. icterohaemorragiae, 12; para el L. Bratislava, 3; para L.canicola, 5; y para el L. grippotyphosa, 1, encontrando una prevalencia serológica para el serovar L. icterohaemorragiae (44,44%. Estos resultados nos pueden ofrecer mayores elementos en la comprensión de la leptospirosis, que es una enfermedad que afecta tanto a los animales como a los humanos y en estos últimos puede causar desde signos poco específicos de hemorragia hasta lesiones renales.

  2. Respuesta de anticuerpos inducidos por la vacuna antimeningocócica cubana VA-MENGOC-BC® frente a la cepa de Neisseria meningitidis B:4:P1.19,15 en adolescentes después de 12 años de inmunizados

    Directory of Open Access Journals (Sweden)

    María A. Camaraza

    2006-12-01

    Full Text Available Se estudió la respuesta de anticuerpos inducida por la vacuna antimeningocócica cubana VA-MENGOC-BC® contra la cepa de Neisseria meningitidis B:4:P1.19,15 mediante el Ensayo Bactericida del Suero (EBS y ELISA de tipo indirecto, para medir anticuerpos contra vesículas de membrana externa (VME de N. meningitidis B a 184 adolescentes de un politécnico de Ciego de Ávila que fueron inmunizados en campañas masivas 12 años antes. Se realizaron extracciones de sangre antes de aplicar la primera dosis (T0, 4 semanas después de ésta (T1 y 4 semanas después de la segunda dosis (T2. Transcurridos 12 años de esta vacunación el 42% de los adolescentes presentó títulos bactericidas ≥ 1:4 frente a la cepa homóloga (B:4:P1.19,15 y el 98% mostró anticuerpos detectables contra las VME. En el EBS; el porcentaje de seroconversión T1/T0 fue del 57% y T2/T0 del 60%. Mediante ELISA la seroconversión fue del 59% y 54%, respectivamente, por lo que se demostró que la aplicación de una sola dosis después de 12 años indujo una respuesta inmune importante que puede sugerir una respuesta anamnésica.

  3. Anticuerpos antitiroperoxidasa y antitransglutaminasa en familiares de primer grado de personas con diabetes tipo 1 y su relación con algunas características clínicas, bioquímicas e inmunológicas Antithyroperoxidase and antitransglutaminase antibodies in first degree relatives of type 1 diabetes persons and its relation to some clinical, biochemical and immunological features

    OpenAIRE

    Levi González Rivero; Eduardo Cabrera Rode; Silvia Elena Turcios Tristá; José Armando Galván Cabrera; Julio César Rodríguez González; Tania Espinosa Reyes; Pedro González Fernández; Manuel Vera González; Celeste Arranz Calzado; Oscar Díaz Horta

    2010-01-01

    INTRODUCCIÓN: los anticuerpos antitiroperoxidasa (AcTPO) y antitransglutaminasa (ATGt) son útiles marcadores de enfermedad tiroidea autoinmune y enfermedad celíaca, respectivamente. Su presencia en familiares de primer grado de personas con diabetes tipo 1 no se ha descrito en Cuba. OBJETIVO: determinar las frecuencias de los AcTPO y ATGt en familiares de primer grado de personas con diabetes tipo 1 y su relación con algunas características clínicas, bioquímicas e inmunológicas. MÉTODOS: en u...

  4. Detección de anticuerpos IgA y PCR como primeras opciones en el diagnóstico de infección perinatal por el VIH-1 IgA antibody detection and PCR as first options in the diagnosis of perinatal HIV-1 infection

    OpenAIRE

    Ma del Carmen Basualdo; Kathya Moran; Patricia Alcántara; Elizabeth González; Esteban Puentes; Carmen Soler

    2004-01-01

    OBJETIVO: Evaluar la sensibilidad y especificidad de la reacción en cadena de la polimerasa y de las pruebas de ELISA e inmunoblot para anticuerpos IgA específicos, como únicos métodos en el diagnóstico de infección perinatal del VIH-1. MATERIAL Y MÉTODOS: Estudio de evaluación comparativa, efectuado entre febrero y octubre de 2001 en la Unidad de Investigación en Retrovirus Humanos de la Universidad Nacional Autónoma de México. Se incluyeron 90 muestras de niños infectados y 153 de no infect...

  5. Resultados de un programa de control de calidad externo del tamizaje serológico de anticuerpos contra Trypanosoma cruzi en donantes de sangre de Brasil

    Directory of Open Access Journals (Sweden)

    Amadeo Sáez-Alquézar

    2003-03-01

    Full Text Available OBJETIVOS: A partir de 1995 en Brasil se iniciaron programas de control de calidad externo de laboratorios de serología (PCCES abiertos a la participación de laboratorios de bancos de sangre públicos y privados. Estos programas se han puesto en práctica tres veces al año desde 1995 y cuentan con la participación de más de 100 entidades. El objetivo de este trabajo es analizar los resultados de los ocho últimos programas de evaluación del tamizaje serológico para la enfermedad de Chagas, que se realizaron entre abril de 1999 y agosto de 2001. MÉTODOS: Participaron en los ocho programas laboratorios de serología de instituciones públicas y privadas. El número de laboratorios participantes que entregó los resultados en cada uno de los ocho PCCES fue de 94, 90, 85, 94, 100, 103, 102 y 116, respectivamente. Al inicio de cada PCCES se envió un panel enmascarado a cada institución participante, con un plazo de 60 días para informar los resultados del procesamiento de las muestras. Se utilizaron pruebas de inmunoadsorción enzimática (ELISA, inmunofluorescencia indirecta (IFI y hemaglutinación indirecta (HAI. Posteriormente, cada institución recibió del centro organizador (PANEL la clave con los resultados correctos para su autoevaluación. Los paneles estaban compuestos por 24 muestras de sueros con diferentes reactividades a los marcadores obligatorios en el tamizaje serológico de donantes de sangre en Brasil, incluido el de muestras negativas. RESULTADOS: La técnica de ELISA fue la más utilizada en el tamizaje (92%-98%. La estrategia de tamizaje más usada por los laboratorios participantes fue la combinación de una prueba de ELISA y una de HAI (58%-83%. La mayoría de los laboratorios participantes obtuvieron resultados correctos en los diferentes programas sin resultados negativos falsos (83,6%-98,1%. De las 5 406 muestras de suero positivas a anti-Trypanosoma cruzi que hubo en los ocho programas, 85 (1,6% fueron

  6. Photon anti bunching versus phantom anti bunching?

    International Nuclear Information System (INIS)

    Miranowicz, A.; Ekert, A.; Ekert, A.; Bajer, J.; Leonski, W.

    1997-01-01

    Photon anti bunching defined by two-time correlation functions has hitherto, to our best knowledge, been considered to constitute a unique, well defined effect. We show explicitly that this is by no means the case. We analyze two of the most famous definitions showing that both anti bunching and bunching effects according to one definition can be accompanied by arbitrary photon correlation effects according to another. As an example we discuss a model of parametric frequency conversion. (authors)

  7. ADCC-Mediated CD56dim NK Cell Responses Are Associated with Early HBsAg Clearance in Acute HBV Infection

    Directory of Open Access Journals (Sweden)

    Wen-Han Yu

    2018-02-01

    Full Text Available Hepatitis B virus (HBV affects up to 400 million people worldwide and accounts for approximately one million deaths per year from liver pathologies. Current treatment regimens are effective in suppressing viremia but usually have to be taken indefinitely, warranting research into new therapeutic approaches. Acute HBV infection in adults almost universally results in resolution of viremia, with the exception of immunocompromised persons, suggesting that the immune response can functionally cure or even eradicate HBV infection. Because immunophenotypic and functional studies have implicated a role for Natural Killer (NK cells in HBV clearance during acute infection, we hypothesized that a distinct NK-cell profile exists in acute HBV infection that could provide information for the mechanism of HBV clearance. Using multivariate flow cytometry, we evaluated the expression of key activating and inhibitory receptors on NK cells, and their ability to respond to classic target cell lines. Multivariate analysis revealed selective perturbation of the CD56 dim NK-cell subset during acute infection, displaying low levels of NKp46+, NKp30+, CD160+ and CD161+ cells. Intriguingly, the CD56 dim NK-cell profile predicted time to HBV surface antigen (HBsAg clearance from the blood, and distinct NK-cell profiles predicted early (NKp30, CD94, CD161 and late clearance (KIR3DL1, CD158a, perforin, NKp46. Finally, functional analysis demonstrated that early and late clearance tracked with elevated degranulation (CD107a or IFNγ production, respectively, in response to ADCC-mediated activation. In conclusion, the cytolytic CD56dim NK-cell subset is selectively activated in acute HBV infection and displays distinct phenotypic and functional profiles associated with efficient and early control of HBV, implicating antibody-mediated cytolytic NK-cell responses in the early control and functional cure of HBV infection.

  8. ADCC-Mediated CD56DIM NK Cell Responses Are Associated with Early HBsAg Clearance in Acute HBV Infection.

    Science.gov (United States)

    Yu, Wen-Han; Cosgrove, Cormac; Berger, Christoph T; Cheney, Patrick C; Krykbaeva, Marina; Kim, Arthur Y; Lewis-Ximenez, Lia; Lauer, Georg M; Alter, Galit

    2018-01-01

    Hepatitis B virus (HBV) affects up to 400 million people worldwide and accounts for approximately one million deaths per year from liver pathologies. Current treatment regimens are effective in suppressing viremia but usually have to be taken indefinitely, warranting research into new therapeutic approaches. Acute HBV infection in adults almost universally results in resolution of viremia, with the exception of immunocompromised persons, suggesting that the immune response can functionally cure or even eradicate HBV infection. Because immunophenotypic and functional studies have implicated a role for Natural Killer (NK) cells in HBV clearance during acute infection, we hypothesized that a distinct NK-cell profile exists in acute HBV infection that could provide information for the mechanism of HBV clearance. Using multivariate flow cytometry, we evaluated the expression of key activating and inhibitory receptors on NK cells, and their ability to respond to classic target cell lines. Multivariate analysis revealed selective perturbation of the CD56 dim NK-cell subset during acute infection, displaying low levels of NKp46+, NKp30+, CD160+ and CD161+ cells. Intriguingly, the CD56 dim NK-cell profile predicted time to HBV surface antigen (HBsAg) clearance from the blood, and distinct NK-cell profiles predicted early (NKp30, CD94, CD161) and late clearance (KIR3DL1, CD158a, perforin, NKp46). Finally, functional analysis demonstrated that early and late clearance tracked with elevated degranulation (CD107a) or IFNγ production, respectively, in response to ADCC-mediated activation. The cytolytic CD56 dim NK-cell subset is selectively activated in acute HBV infection and displays distinct phenotypic and functional profiles associated with efficient and early control of HBV, implicating antibody-mediated cytolytic NK-cell responses in the early control and functional cure of HBV infection.

  9. Títulos de anticuerpos antisulfátido en pacientes con cardiopatía chagásica crónica y con otras formas de cardiopatía Antisulfatide antibody titers in patients with chronic Chagas heart disease and other forms of heart disease

    Directory of Open Access Journals (Sweden)

    Rando García

    1998-04-01

    Full Text Available Aún no se ha podido encontrar un tratamiento específico para la enfermedad de Chagas, a pesar de su endemicidad en extensas zonas de la Región de las Américas. En estudios anteriores se ha propuesto la posibilidad de que el galactocerebrósido sulfatado presente en Trypanosoma cruzi podría actuar como inmunógeno para producir las concentraciones elevadas de anticuerpos antisulfátido que se han encontrado en pacientes con infección crónica por ese parásito. Ese fenómeno podría ser importante en la patogenia de los síntomas cardíacos o del sistema nervioso periférico que caracteriza a la enfermedad de Chagas, que es la primera causa de miocarditis en Centroamérica y América del Sur y la segunda causa de insuficiencia cardíaca en algunos países de esas subregiones. El presente estudio se realizó con objeto de determinar si había diferencia entre pacientes con la enfermedad de Chagas y otros pacientes sin la enfermedad con respecto a la presencia de anticuerpos antisulfátido y describe las concentraciones de anticuerpos antisulfátido que se encontraron en 124 pacientes (74 hombres y 50 mujeres de 15 a 94 años de edad hospitalizados en el Servicio de Cardiología del Hospital Vargas de Caracas, durante el período del 1 de julio de 1993 al 30 de junio de 1995. Los títulos de anticuerpos antisulfátido se midieron con la técnica del inmunoensayo enzimático (ELISA usando como antígeno cerebrósido sulfatado obtenido de cerebro de bovino. De los 124 pacientes estudiados, 39 (31,5% eran chagásicos y mostraron títulos de anticuerpos antisulfátido más altos que los 85 no chagásicos (P = 0,0298 y que un grupo control de 28 sujetos aparentemente sanos (P = 0,0035. Las concentraciones séricas de anticuerpos antisulfátido en pacientes con otras formas de cardiopatía también se compararon con las del grupo control y se encontraron títulos significativamente altos en sujetos con cardiopatía isquémica aguda (P = 0

  10. Evaluación de la calidad de los reactivos que se utilizan en la tecnica de contrainmunoelectroforesis para la determinación de anticuerpos antirrábicos Evaluation of the quality of the reagents employed in the counterimmunoelectrophoresis technique for the determination of rabies antibodies

    Directory of Open Access Journals (Sweden)

    A. M. Díaz

    1991-02-01

    Full Text Available En este estudio se comprobó que el Instituto Butantan produce antígenos y sueros indicadores que se pueden utilizar con éxito en la prueba de contrainmunoelectroforesis para titular anticuerpos antirrábicos en personas inmunizadas. No se pudieron demostrar diferencias estadísticamente significativas entre los resultados de las pruebas de estandarización realizadas en el Instituto Butantan y las pruebas de control de referencia llevadas a cabo en el Centro Panamericano de Zoonosis. Se propone que el Instituto Butantan produzca y distribuya a nivel nacional los reactivos para que los laboratorios de diagnóstico apliquen la técnica de contrainmunoelectroforesis para la determinación de anticuerpos antirrábicos.This study demonstrated that the antigens and indicator sera produced by the Butantan Institute may be employed with success in the counterimmunoelectrophoresis technique for the titration of rabies antibodies in sera from immunized individuals. No statistically significant differences were demonstrated between the results obtained in the standardization tests carried out at the Butantan Institute and the reference control tests performed at the Pan American Zoonoses Center. It is proposed that the Butantan Institute be in charge of the production and distribution of these reagents at the national level.

  11. Anti-Vaccination Movement

    OpenAIRE

    Chapman, Jonathan

    2010-01-01

    The current anti-vaccination movements that have established themselves in the United States as well as other regions in the world are like a hydra of discourse. Right when one effective measure is created to convince people to vaccinate two more anti-vaccination movements sprout up in its place. These anti-vaccination movements are driven by cultural beliefs, ideologies, medical exemption laws, non-medical exemption laws, distrust of the government, distrust of large pharmaceutical companies...

  12. The Impact of XmnI-HBG2, BCL11A and HBS1L-MYB Single Nucleotide Polymorphisms on Hb F Variation of Hematologically Normal Iranian Individuals.

    Science.gov (United States)

    Keyhani, Elaheh; Jafari Vesiehsari, Mahjoobeh; Talebi Kakroodi, Setareh; Darabi, Elham; Zamani, Fahimeh; Karimlou, Masoud; Kamali, Koorosh; Neishabury, Maryam

    2016-06-01

    The impact of Hb F on severity of sickle cell disease and β-thalassemia (β-thal) is well documented. The XmnI-HBG2, BCL11A and HBS1L-MYB single nucleotide polymorphisms (SNPs) have been introduced as the most important factors causing variation in fetal hemoglobin (Hb F) levels in different population studies. However, the extent of their effect could be population-specific. In this study, multivariate linear regression analysis was used to evaluate the association of Hb F with age, sex, and eight SNPs, including XmnI-HBG2, four BCL11A, two HBS1L-MYB SNPs and the polymorphic palindromic 5' hypersensitive 4-locus control region (5'HS4-LCR). One hundred and twenty-two hematologically normal individuals, from a previous study cohort, constituted our study population. In multivariate regression analyses, no association of Hb F was observed with age or sex of the individuals and SNPs in this study. We conducted a univariate regression analysis to further investigate the results, which among all the factors only detected XmnI-HBG2 and 5'HS4 SNPs as significant modifiers of Hb F. The significance of these two factors disappeared in a bivariate analysis. These results suggest that either XmnI-HBG2 or 5'HS4-LCR have a stronger contribution in Hb F variations of the Iranian population than BCL11A and HBS1L-MYB SNPs. Furthermore, the effect of low population size and technical limitations on obtained results could not be ruled out.

  13. Evaluation of hepatic functional gain by hepato-biliary scintigraphy (HBS) after portal embolization; Evaluation du gain functionnel hepatique par la scintigraphie hepato-biliaire (SHB) apres embolisation portale

    Energy Technology Data Exchange (ETDEWEB)

    Zerbib, E.; Azoulay, D.; Wartski, M.; Castaing, D.; Bismuth, H. [Medecine Nucleaire CCML, 92350 Le Plessis-Robinson, CHB, Hopital Paul Brousse, 94804 Villejuif Cedex (France)

    1997-12-31

    The partial hepatic extirpation is an alternative therapeutics in malign tumors of liver. The post-surgery risk of hepato-cellular insufficiency (HCI) is important when the extracted volume is higher than 70 - 80% of the total volume of a non-cirrhotic liver. In this case, sometimes, a homolateral-to-tumor portal embolization (PE) was proposed in order to induce an atrophy of the embolized side and a compensating hypertrophy of the contralateral side. The HBS was proposed as a method for evaluated the functional gain after PE. Six patients have benefited by 2 HBS: one of them before PE, the others, 3 weeks after. The dynamic acquisition was achieved after intravenous injection of 300 MBq of {sup 99m}Tc - tBIDA, on a DST (SMV) camera equipped with HRBE collimators. The regions of interest (ROI) corresponding to right and left liver, were drawn on an anterior and posterior image of the `parenchymatous` time (the forth or fifth minute), allowing the calculation of the functional ratio by arithmetic mean. The gain of the functional ratio of healthy liver was in average of 7% (ranging from 1 to 13%). These results appear to be comparable with those from scanner in 5 cases but different in one case (functional gain without volumetric gain at scanner); this last patient was finally successfully operated. Totally, the surgery indication was posed in these 6 patients, one of whom, solely, on arguments of scintigraphic quantification. The HBS allows estimating the variations in functional ratios before and after PE. This is a supplementary tool in the evaluation of the feasibility of a major hepatectomy after PE

  14. Anti-matter factory

    CERN Document Server

    Michaut, C

    2003-01-01

    In 1928 Paul Dirac predicted the existence of anti-matter as a consequence of his theory. The first experimental fact that supported this idea was the discovery of the positron in 1932. Anti-protons were produced in 1955 at the Bevatron facility in Berkeley and anti-neutrons the following year. Since 1964 scientists know that matter and anti-matter differ not only from their electrical charge but also from their behaviour concerning weak interaction. In 2002 the CERN announced the production of more than 50.000 atoms of anti-hydrogen which was a breakthrough because the last attempt in 1995 ended with the creation of only 9 atoms of anti-hydrogen whose speed neared the velocity of light while the speed of the last ones created is 100.000 times smaller. The next step is to carry out experiments in order to measure the spectra of anti-hydrogen in a very accurate manner and to test the effect of gravity on anti-matter. (A.C.)

  15. Teniosis y detección de anticuerpos anticisticerco en personas de una comunidad rural del estado de Guerrero Taeniosis and detection of antibodies against cysticerci among inhabitants of a rural community in Guerrero State, Mexico

    Directory of Open Access Journals (Sweden)

    José Juan Martínez-Maya

    2003-04-01

    Full Text Available OBJETIVO: Determinar la frecuencia de portadores de Taenia sp. y su relación con el diagnóstico de cisticercos en humanos en una comunidad rural del estado de Guerrero, México. MATERIAL Y MÉTODOS: Para detectar portadores de Taenia sp. se analizaron 403 muestras de heces de personas, por medio de ELISA para coproantígenos de Taenia sp., así como 92 muestras de suero para detectar anticuerpos anticisticerco mediante inmunoelectrotransferencia. El diseño del estudio fue transversal y se llevó a cabo durante 1998. Se hizo estadística descriptiva y se estimó razón de momios. RESULTADOS: De 403 muestras de heces evaluadas, cinco resultaron positivas (1.2 %. Sólo en dos de las cinco personas positivas se obtuvo el cestodo adulto. En 3 (3.26% de los 92 sueros se encontraron anticuerpos anticisticerco. Del total de sueros, 17 fueron de las personas con diagnóstico positivo a teniosis por coproantígenos o que cohabitaban con ellos (primer grupo, los restantes 75 provenían de personas en quienes no se detectaron casos en las viviendas (segundo grupo. En el primer grupo se detectaron 2 (11.8% sueros positivos, mientras que en el segundo sólo 1 (1.3% (RM= 9.87, I.C 0.64-295.56, p= 0.086. CONCLUSIONES: La dificultad para obtener el parásito adulto en las personas positivas a coproantígenos puede deberse a características propias de éste que dificultan su expulsión, a que la permanencia del cestodo en su huésped es menor a la esperada o a que el tratamiento fue insuficiente para obtener el parásito, o bien, a problemas de especificidad de la prueba. Es necesario realizar estudios tendientes a evaluar estas posibilidades, lo cual permitiría conocer mejor la dinámica de transmisión de esta parasitosis, con el fin de establecer medidas de prevención y control, además de poder comparar con mayor veracidad la eficacia de las pruebas diagnósticas en condiciones de campo.OBJECTIVE: To assess the frequency of Taenia solium carriers and

  16. PREVALENCE OF ANTI-HBC IN SLOVENIAN BLOOD DONORS AND THE IMPACTON BLOOD SCREENING

    Directory of Open Access Journals (Sweden)

    Snežana Levičnik Stezinar

    2008-04-01

    Among screened donors, the anti-HBc incidence rate was 179/5959 (3.00 %. Therewere 55 samples that were anti-HBc positive only (69.83 % of anti-HBc pos, 0.90 % of alldonations. The number of units positive for both antibodies was 124 (2.10 % of all testeddonations. No significant difference was noted in the prevalence between male (3.00 %and female (2.99 %. As expected, there was a raise in frequency from 0.78 % in a group of18–29 years to 5.82 % in a group 50–65 years. In the study group there was one donorwho was HBsAg carrier.Conclusion According to the results of this study, Slovenia is ranged among the countries with mediumanti-HBc prevalence rate. Screening of donors would additionally contribute to the higherblood safety. The higher cost due to testing and destroying units is not acceptable, especiallyafter the implementation of screening for HBV DNA and excluding the units that are reallyinfectious

  17. Anti-tuberculosis therapy-induced hepatotoxicity among Ethiopian HIV-positive and negative patients.

    Directory of Open Access Journals (Sweden)

    Getnet Yimer

    2008-03-01

    Full Text Available To assess and compare the prevalence, severity and prognosis of anti-TB drug induced hepatotoxicity (DIH in HIV positive and HIV negative tuberculosis (TB patients in Ethiopia.In this study, 103 HIV positive and 94 HIV negative TB patients were enrolled. All patients were evaluated for different risk factors and monitored biochemically and clinically for development of DIH. Sub-clinical hepatotoxicity was observed in 17.3% of the patients and 8 out of the 197 (4.1% developed clinical hepatotoxicity. Seven of the 8 were HIV positive and 2 were positive for HBsAg.Sub-clinical hepatotoxicity was significantly associated with HIV co-infection (p = 0.002, concomitant drug intake (p = 0.008, and decrease in CD4 count (p = 0.001. Stepwise restarting of anti TB treatment was also successful in almost all the patients who developed clinical DIH. We therefore conclude that anti-TB DIH is a major problem in HIV-associated TB with a decline in immune status and that there is a need for a regular biochemical and clinical follow up for those patients who are at risk.

  18. Anti-HBs levels in infants of hepatitis B carrier mothers after delayed active immunization with recombinant vaccine concomitant with DTP-polio vaccine: Is there need for a second dose of HBIg?

    NARCIS (Netherlands)

    P.M. Grosheide (Pia Maria); R. Del Canho (R.); M. Voogd (M.); R.A. Heijtink; S.W. Schalm (Solko)

    1994-01-01

    textabstractThe need for an additional dose of hepatitis B immune globulin (HBIg) was studied by comparing infants receiving 1 ml HBIg at birth followed by hepatitis B immunization, concomitant with DTP-polio vaccine, at 3, 4, 5 and 11 months (schedule E), with infants receiving the same schedule

  19. Simultaneous passive and active immunization against hepatitis B: noninterference of hepatitis B immune globulin with the anti-HBs response to reduced doses of heat-inactivated hepatitis B vaccine

    NARCIS (Netherlands)

    Lelie, P. N.; Reesink, H. W.; Grijm, R.; de Jong-van Manen, S. T.; Reerink-Brongers, E. E.

    1986-01-01

    The effect of simultaneous administration of hepatitis B immune globulin on the antibody response to a low dose of heat-inactivated hepatitis B vaccine was investigated in 175 health care workers. Subjects were divided into four groups: Groups I and II received 3 monthly injections of a reduced dose

  20. Anti-convulsants and anti-depressants.

    Science.gov (United States)

    Dickenson, A H; Ghandehari, J

    2007-01-01

    Damage to a nerve should only lead to sensory loss. While this is common, the incidence of spontaneous pain, allodynia and hyperalgesia indicate marked changes in the nervous system that are possible compensations for the loss of normal function that arises from the sensory loss. Neuropathic pain arises from changes in the damaged nerve which then alter function in the spinal cord and the brain and lead to plasticity in areas adjacent to those directly influenced by the neuropathy. The peripheral changes drive central compensations so that the mechanisms involved are multiple and located at a number of sites. Nerve damage increases the excitability of both the damaged and undamaged nerve fibres, neuromas and the cell bodies in the dorsal root ganglion. These peripheral changes are substrates for the ongoing pain and the efficacy of excitability blockers such as carbamazepine, lamotrigine and mexiletine, all anti-convulsants. A better understanding of ion channels at the sites of injury has shown important roles of particular sodium, potassium and calcium channels in the genesis of neuropathic pain. Within the spinal cord, increases in the activity of calcium channels and the receptors for glutamate, especially the N-methyl-D-aspartate (NMDA) receptor, trigger wind-up and central hyperexcitability. Increases in transmitter release, neuronal excitability and receptive field size result from the damage to the peripheral nerves. Ketamine and gabapentin/pregabalin, again with anti-convulsant activity, may interact with these mechanisms. Ketamine acts on central spinal mechanisms of excitability whereas gabapentin acts on a subunit of calcium channels that is responsible for the release of pain transmitters into the spinal cord. In addition to these spinal mechanisms of hyperexcitability, spinal cells participate in a spinal-supraspinal loop that involves parts of the brain involved in affective responses to pain but also engages descending excitatory and inhibitory

  1. Serodiagnóstico de giardiosis: identificación de inmunoglobulina G anti-Giardia duodenalis en suero mediante ELISA

    Directory of Open Access Journals (Sweden)

    Sofía Duque

    2001-09-01

    Full Text Available El diagnóstico de la infección por Giardia duodenalis se basa en la identificación de quistes o trofozoítos en materia fecal, alcanzando una sensibilidad máxima de 85% en muestras seriadas, debido a la excreción intermitente de quistes. Aunque la determinación de anticuerpos no permite diferenciar entre una infección pasada y una reciente, si podría ayudar en el diagnóstico diferencial. El presente estudio tuvo como objetivos estandarizar y evaluar una prueba inmunoenzimática (ELISA para la detección de anticuerpos tipo IgG anti-Giardia duodenalis en sueros de pacientes con la infección. La preparación del antigeno se hizo a partir de trofozoitos cultivados que, inicialmente, fueron obtenidos del intestino de gerbils (Meriones unguiculatus infectados experimentalmente con quistes purificados a partir de muestras positivas de materia fecal humana. Para la estandarización y evaluación de la prueba, se emplearon 60 sueros de pacientes con giardiosis comprobada parasitológicamente (muestras positivas y 47 sueros de cordón umbilical recién cortado (muestras negativas. Como conjugado, se utilizó anti-lgG humana ligada a fosfatasa alcalina. La concentración óptima de antígeno de trofozoíto de G. duodenalis fue de 15 ug/ml y las diluciones óptimas de suero y conjugado fueron 1:25 y 1:400, respectivamente. El valor del punto de corte (absorbancia fue 0,300. Los parámetros de la prueba fueron: sensibilidad, 98,3% (intervalo de confianza de 95% (lC 95%: 89,9%-99,974; especificidad, 957% (IC 95%:84,3%-99,3%; valor predictivo positivo, 96,7% (IC 95%: 87,6%-99,4%, y valor predictivo negativo, 97,8% (IC 95%: 87,0%-99,9%. La prueba ELlSA descrita contribuirá a mejorar el diagnóstico de giardiosis y podrá ser empleada para estudios epidemiológicos de seroprevalencia.

  2. Seroprevalence of antibodies against rubella virus in pregnant women in Haiti Seroprevalencia de anticuerpos contra el virus de la rubéola en mujeres embarazadas de Haití

    Directory of Open Access Journals (Sweden)

    Olbeg Y. Désinor

    2004-03-01

    ó entre febrero y mayo de 2002. Se evaluó la presencia de anticuerpos de tipo IgG específicos contra la rubéola en 503 embarazadas mediante un inmunoensayo enzimático. Posteriormente se excluyó a 8 mujeres debido a que no sabían su edad. RESULTADOS: De las 495 participantes estudiadas, 471 (95,2% tenían seropositividad; solo 24 de ellas (4,8% mostraron seronegatividad (susceptibilidad. Se encontraron menos mujeres seronegativas entre las residentes de Puerto Príncipe (17 de 426, 4,0% que entre las residentes de zonas rurales (7 de 69, 10,1% (P = 0,02. En relación con la edad, 81 (16,4% de las 495 mujeres embarazadas estudiadas eran menores de 21 años. CONCLUSIONES: Con este estudio se da el primer paso hacia evaluar la prevalencia de la infección por el virus de la rubéola en mujeres haitianas y hacia el enfrentamiento de un problema de salud que todavía no ha sido reconocido en toda su magnitud: el síndrome de la rubéola congénita en Haití. Se recomienda emprender estudios adicionales con muestreos aleatorios que abarquen una fracción significativa de mujeres provenientes de zonas rurales.

  3. Anti pp interactions in anti p atoms

    International Nuclear Information System (INIS)

    Poth, H.

    1975-01-01

    The x-rays of anti p atoms were recorded to trace out a strong interaction shift and width to the electromagnetic energy and the radiation width of the low-lying levels. The energy of the levels and consequently the energy of the transitions was calculated by solving numerically the Dirac equation with an electromagnetic potential, taking into account the finite size of the nucleus and the vacuum polarization. Due to the lack of sufficient data, the antiproton-nucleon interaction was not well understood at low energies. In order to separate antiproton-proton from antiproton-neutron interactions, the studies of isotope effects were proposed. (L.M.K.)

  4. The Anti-Landscape

    DEFF Research Database (Denmark)

    There have always been some uninhabitable places, but in the last century human beings have produced many more of them. These anti-landscapes have proliferated to include the sandy wastes of what was once the Aral Sea, severely polluted irrigated lands, open pit mines, blighted nuclear zones, coa...

  5. A rare case of Addison's disease, hepatitis, thyreoiditis, positive IgG anti-tissue transglutaminase antibodies and partial IgA deficiency.

    Science.gov (United States)

    Baleva, Marta P; Mihaylova, Snejina; Yankova, Petja; Atanasova, Iliana; Nikolova-Vlahova, Milena; Naumova, Elissaveta

    2016-01-01

    Selective IgA deficiency (IgAD) is the most prevalent type of primary immune deficiencies, but partial IgA deficiency is even more common. Addison's disease is a rare condition associated with primary adrenal insufficiency due to infection or autoimmune destruction of the adrenals. The association between IgA deficiency and Addison's disease is very rare. We observed a 22-year-old male patient with marked darkening of the skin, especially on the palms and areolae, jaundice on the skin and sclera, astheno-adynamia, hypotension (80/50 mm Hg), and pain in the right hypochondrium. The laboratory investigations revealed increased serum levels of total and indirect bilirubin, AST, ALT, GGT and LDH, negative HBsAg, anti-HBc IgM, anti-HCV and anti-HAV IgM, very low serum IgA levels (0.16 g/l) with normal IgG and IgM, negative ANA, ANCA, AMA, LKM-1, anti-GAD-60, anti-IA-2, anti-thyroglobulin antibodies, a mild increase in anti-TPO antibodies titer, a marked increase in IgG anti-tissue transglutaminase antibodies, with no typical changes in cellular immunity, negative T-SPOT-TB test, HLA - A*01; B*08; DRB1*03; DQB1*02, karyotype - 46, XY. We present a rare case of partial IgA deficiency with Addison's disease, hepatitis, thyroiditis and positive anti-tissue transglutaminase antibodies. IgAD and some autoimmune disorders share several predisposing HLA genes, thus explaining the increased prevalence of IgAD in certain patient groups.

  6. Diagnostic incidence of the presence of positive HBsAg: epidemiologic, clinical, and virological characteristics Incidencia diagnóstica de AgHBs positivo: Características epidemiológicas, clínicas y virológicas

    Directory of Open Access Journals (Sweden)

    Elvira Poves Martínez

    2012-01-01

    Full Text Available Objective: to analyze the epidemiological, clinical, and virological characteristics of patients newly diagnosed with active hepatitis B virus (HBV infection based on the presence of positive hepatitis B surface antigen (HBsAg in the digestive diseases department of a district hospital. Patients and methods: we performed a 3-year prospective study in patients newly diagnosed with HBV infection. We analyzed epidemiological, clinical, and virological characteristics, complete HBV markers, quantification of HBV DNA, and infection by hepatitis delta virus. We performed genotyping and resistance testing in patients with a high viral load. Results were obtained for patients who required liver biopsy. Results: we diagnosed 213 patients (18.8/10,000 inhabitants/year. Men accounted for 61%, and 59% were aged 20 to 40 years. Immigrants accounted for 53% of the population: 46% were from Rumania and 37% from Sub-Saharan African countries. At diagnosis, 2.3% had acute hepatitis (all with jaundice and 3.3% had cirrhosis with portal hypertension. With the exception of cases of acute hepatitis, positive HBeAg was observed in 9%. Serum transaminase levels were normal in 62.2% of patients, HBV DNA was > 2,000 IU/mL in 33.8%, and delta virus was present in 3.3%. Genotyping and resistance testing were performed in 70 patients: the most common genotype was D, followed by A. Resistance was detected at baseline in only 2 cases: to adefovir in one case and to entecavir in another. Among the 36 biopsies performed, 32.4% showed inflammatory activity ≥ 2, and 23.5% had fibrosis ≥ 2 according to the METAVIR scoring system. According to clinical practice, specific treatment for HBV infection was necessary (any reason in 17.4% of those diagnosed (3 patients per 100,000 inhabitants/year. Conclusions: despite prevention and vaccination, HBV infection is a health problem that most commonly affects the immigrant population and men. Serum transaminase levels are normal in 62

  7. Comparison of an enzyme-immunoassay with a radio-immunoassay ...

    African Journals Online (AJOL)

    immunoassay (EIA) method for detecting the hepatitis markers anti-HBs, anti-HBc and HBsAg. The results indicated that the RIA and EIA were comparable for the HBsAg marker but that the RIA test was more sensit!ve for anti-HBs and more ...

  8. Investigation of the anti-inflammatory and anti- nociceptive activities ...

    African Journals Online (AJOL)

    PRECIOUS

    2009-06-15

    Jun 15, 2009 ... (1998) Antinociceptive and anti-inflammatory effects of Sambucus ebulus rhizome extract in rats. J. Ethnopharmacol. 61: 229-235. Barros IMC, Lopes LDG, Borges MOR, Borges ACR, Ribeiro MNS,. Freire SMF (2006). Anti-inflammatory and anti-nociceptive activities of Pluchea quitic (D.C) ethanolic extract.

  9. [Anti-HBV effects of genetically engineered replication-defective HBV with combined expression of antisense RNA and dominant negative mutants of core protein and construction of first-generation packaging cell line for HBV vector].

    Science.gov (United States)

    Sun, Dian Xing; Hu, Da Rong; Wu, Guang Hui; Hu, Xue Ling; Li, Juan; Fan, Gong Ren

    2002-08-01

    To explore the possibility of using HBV as a gene delivery vector, and to test the anti-HBV effects by intracellular combined expression of antisense RNA and dominant negative mutants of core protein. Full length of mutant HBV genome, which expresses core-partial P fusion protein and/or antisense RNA, was transfected into HepG2.2.15 cell lines. Positive clones were selected and mixed in respective groups with hygromycin in the culture medium. HBsAg and HBeAg, which exist in the culture medium, were tested by ELISA method. Intracellular HBc related HBV DNA was examined by dot blot hybridization. The existence of recombinant HBV virion in the culture medium was examined by PCR. Free of packaging signal, HBV genome, which express the HBV structural proteins including core, pol and preS/S proteins, was inserted into pCI-neo vector. HepG2 cell lines were employed to transfect with the construct. G418 selection was done at the concentration of 400mug/ml in the culture medium. The G418-resistant clones with the best expression of HBsAg and HBcAg were theoretically considered as packaging cell lines and propagated under the same conditions. It was transfected with plasmid pMEP-CPAS and then selected with G418 and hygromycin in the culture medium. The existence of recombinant HBV virion in the culture medium was examined by PCR. The mean inhibitory rates of HBsAg were 2.74% 3.83%, 40.08 2.05% (t=35.5, PDNA were 0, 82.0%, 59.9%, and 96.6%, respectively. Recombinant HB virion was detectable in the culture medium of all the three treatment groups. G418-resistant HBV packaging cell line, which harbored an HBV mutant whose packaging signal had been deleted, was generated. Expression of HBsAg and HBcAg was detectable. Transfected with plasmid pMEP-CPAS, it was found to secrete recombinant HB virion and no wild-type HBV was detectable in the culture medium. It has stronger anti-HBV effects by combined expression of antisense RNA and dominant negative mutants than by individual

  10. Pure anti-oestrogens.

    Science.gov (United States)

    Hermenegildo, C; Cano, A

    2000-01-01

    Pure anti-oestrogens are a group of at least five new compounds which are able to antagonize the effects of oestrogen in all tissues and species studied. The mechanism by which the pure anti-oestrogens produce their effects remains in question, but all of them are competitive antagonists of the oestrogen receptors and, moreover, have been proposed to block the shuttling of oestrogen receptors into the cell nucleus. When studied in vitro, these compounds are able to block the oestrogen-stimulated growth of breast cancer cells. In animals, their ability to block the effects of oestrogen on breast, uterus, bone, cardiovascular system and other reproductive-associated tissues has been demonstrated. ICI 182780 has been used in preliminary clinical trials in women with advanced, tamoxifen-resistant breast cancer with promising results. Clinical trials with EM-800 are under way to assess the safety and tolerance and to obtain information on its efficacy in patients who have already been treated with tamoxifen. It seems reasonable to assume that pure anti-oestrogens will be a good alternative to tamoxifen in the treatment of breast cancer and also in some non-malignant gynaecological diseases.

  11. Insane anti-Membranes?

    International Nuclear Information System (INIS)

    Giecold, Gregory; Orsi, Francesco; Puhm, Andrea

    2014-01-01

    The backreactions of anti-branes on a variety of supergravity backgrounds have been shown in a recent series of papers to be riled by some unexplained flux singularities. All of the situations studied so far involve backgrounds with (close to) AdS-asymptotics. It is the purpose of this work to study the backreaction of anti-M2 branes on a background exhibiting a different UV behavior: the so-called A 8 regular solution of eleven-dimensional supergravity that we consider has “Taub-NUT type' asymptotics. As it turns out, some subleading infrared singularities are inevitable; they cannot be naturally ascribed to the anti-branes backreacting on this background. Moreover, our configuration does not involve smeared branes. This lends further credence to the work of Bena et al. http://dx.doi.org/10.1007/JHEP09(2013)142 suggesting that the singularities encountered are in no way remnants of smearing that would wash away once brane polarization is taken into account

  12. Anti-parallel triplexes

    DEFF Research Database (Denmark)

    Kosbar, Tamer R.; Sofan, Mamdouh A.; Waly, Mohamed A.

    2015-01-01

    -parallel TFO strand was modified with Y with one or two insertions at the end of the TFO strand, the thermal stability was increased 1.2 °C and 3 °C at pH 7.2, respectively, whereas one insertion in the middle of the TFO strand decreased the thermal stability 1.4 °C compared to the wild type oligonucleotide......The phosphoramidites of DNA monomers of 7-(3-aminopropyn-1-yl)-8-aza-7-deazaadenine (Y) and 7-(3-aminopropyn-1-yl)-8-aza-7-deazaadenine LNA (Z) are synthesized, and the thermal stability at pH 7.2 and 8.2 of anti-parallel triplexes modified with these two monomers is determined. When, the anti...... chain, especially at the end of the TFO strand. On the other hand, the thermal stability of the anti-parallel triplex was dramatically decreased when the TFO strand was modified with the LNA monomer analog Z in the middle of the TFO strand (ΔTm = -9.1 °C). Also the thermal stability decreased...

  13. Valor de la determinación de anticuerpos antitiroideos plasmáticos para el diagnóstico de tiroiditis crónica autoinmune Value of the determination of plasmatic antithyroid antibodies for the diagnosis of chronic autoimmune thyroiditis

    Directory of Open Access Journals (Sweden)

    Francisco Ochoa Torres

    2001-08-01

    Full Text Available Se valoró la eficacia de los métodos de detección de anticuerpos humorales empleados en el Instituto Nacional de Endocrinología para el diagnóstico de TCA. Se estudió un grupo de 100 pacientes con bocio y la sospecha clínica de que padecieran de TCA. A cada uno se le realizó una biopsia con aguja fina (BAF, que se tomó como la regla de oro del diagnóstico, determinación de anticuerpos plasmáticos antitiroglobulina por el método del TRC y antimicrosomales (AMS, para hacer una validación estadística de ambos métodos; sensibilidad, especificidad, valor predictivo para casos positivos, valor predictivo para casos negativos y el índice general de eficacia diagnóstica. Se hallaron 70 pacientes con resultados positivos de TCA por la BAF y 30, negativos. La sensibilidad de ambos métodos de detección de anticuerpos humorales fue pobre (45,7 % para el AMS y 30 % para el TRC. Aún aplicados los dos al mismo paciente no sobrepasó del 60 %. La especificidad fue mucho mejor para ambos métodos (73,3 % para la AMS, 90 % para el TRC y 70 % para ambos métodos juntos. El valor predictivo para casos positivos fue bueno (80 % para AMS, 87,5 % para TRC y 82,3 % para los métodos de conjunto y el predictivo para casos negativos fue 36,3, 35,5 y 42,9 %, respectivamente. La eficacia general fue tan solo del 54 % para el TRC, 48 % para AMS y 63 % para ambos métodos de conjunto. Se concluyó que estos métodos son útiles para reafirmar el diagnóstico cuando resultan positivos, pero de poca utilidad para asegurar que no se trata de esta afección cuando resultan negativosThe effectiveness of the humoral antibody detection methods used in the diagnosis of chronic autoimmune thyroiditis (CAT in the National Institute of Endocrinology was assessed. A group of 100 patients with goitier and presenting with clinical suspicion of CAT was studied. A fine needle biopsy was performed on each of the patients, which was taken as the golden rule of the

  14. Anti-semitism and Xenophobia.

    Science.gov (United States)

    Kogan, Ilany

    2017-12-01

    This paper explores the link between anti-Semitism and xenophobia from a psychoanalytic prism. The author claims that anti-Semitism is a particular form of xenophobia directed against Jews, with some unique characteristics. She first introduces some basic notions about xenophobia, and then discusses the origins of anti-Semitism as they are viewed in early psychoanalytic thinking and in contemporary psychoanalysis. The article explores the nature of anti-Semitism in society today, and presents some reflections on the interplay between anti-Semitism and other kinds of xenophobia.

  15. [HLA and hypo-responsivity to anti-HBV vaccination (genetic study of non-responder subjects to anti-hepatitis B viral vaccine)].

    Science.gov (United States)

    Panichi, G; Cappellacci, S; Mola, A; Morellini, M; Lulli, P; Pescini, A

    1986-01-01

    The control of the immunization due to hepatitis B vaccines (HB-VAX and HEVAC B) showed that a low percentage of healthy adults vaccinated develop a non protective title of HBsAb or do not produce antibodies. The correlation between immunity and HLA has already been demonstrated: HLA is at the base of individual immunological response; this correlation directed our genetic study of low-responders or non-responders patients to anti-HBV vaccine. In our study 11 out of 97 subjects vaccinated (11.34%) with HB VAX or HEVAC B resulted hyporesponsive and underwent complete HLA typing to verify the relation between immune deficiency response and genetic system. There was an increase in phenotype HLA-DR7 incidence, with respect to a non-selective population and a decrease of HLA-DR1, as it has already been mentioned in the literature, the variations were not statistically significant taking into account the exiguity of the samples considered.

  16. Triply heavy tetraquark states with the QQ anti Q anti q configuration

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Kan; Liu, Xiang [Lanzhou University, School of Physical Science and Technology, Lanzhou (China); Lanzhou University and Institute of Modern Physics of CAS, Research Center for Hadron and CSR Physics, Lanzhou (China); Liu, Yan-Rui; Wu, Jing [Shandong University, School of Physics and Key Laboratory of Particle Physics and Particle Irradiation (MOE), Jinan (China); Zhu, Shi-Lin [Peking University, School of Physics and State Key Laboratory of Nuclear Physics and Technology, Beijing (China); Collaborative Innovation Center of Quantum Matter, Beijing (China); Peking University, Center of High Energy Physics, Beijing (China)

    2017-01-15

    In the framework of the color-magnetic interaction, we systematically investigate the mass splittings of the QQ anti Q anti q tetraquark states and estimate their rough masses in this work. These systems include the explicitly exotic states cc anti b anti q and bb anti c anti q and the hidden exotic states cc anti c anti q, cb anti b anti q, bc anti c anti q, and bb anti b anti q. If a state around the estimated mass region can be observed, its nature as a genuine tetraquark is favored. The strong decay patterns shown here will be helpful to the experimental search for these exotic states. (orig.)

  17. Encefalitis aguda mediada por anticuerpos contra el receptor ionotrópico de glutamato activado por N-metil-D-aspartato (NMDAR: análisis de once casos pediátricos en Argentina (Premio Benito Yelín

    Directory of Open Access Journals (Sweden)

    Elisa Pérez

    2013-09-01

    Full Text Available Las encefalitis son procesos inflamatorios de diverso origen, entre las cuales se incluyen las autoinmunes. La identificación de anticuerpos contra el receptor N-metil-D-aspartato permitió la caracterización clínico-inmunológica de una entidad susceptible a tratamiento inmunomodulador. Descripta originalmente en mujeres jóvenes, asociada a teratoma de ovario, es hoy una entidad reconocida en niños aun en ausencia de tumores detectables. El objetivo del trabajo, realizado a través de la revisión de historias clínicas, fue describir las características clínicas, evolutivas y los hallazgos en estudios complementarios de once niños con diagnóstico confirmado de esta entidad a través de la identificación de los anticuerpos específicos. Todos debutaron con síntomas psiquiátricos, en nueve de ellos asociando a convulsiones, y dos a movimientos extrapiramidales. En su evolución, todos presentaron compromiso del lenguaje, nueve, síntomas autonómicos graves, en uno con hipoventilación y requerimientos de ARM. La RM de cerebro fue anormal en tres. Ocho tuvieron EEG con asimetría del voltaje y/o amplitud, tres de ellos presentaron espigas. En el LCR seis tuvieron pleocitosis y tres de siete bandas oligoclonales positivas. Cinco, serologías IgM para micoplasma. El aumento de CPK en coincidencia con antipsicóticos ocurrió en cinco. Con el tratamiento inmunomodulador, cinco tuvieron recuperación total, tres, alteraciones conductuales/cognitivas y uno déficits severo. Un paciente resolvió su cuadro clínico sin tratamiento. En ninguno se detectó tumor asociado. Concluimos que frente a un niño con encefalopatía aguda y clínica compatible con esta entidad, una vez descartada causa infecciosa, se debe comenzar tratamiento inmunomodulador precozmente, evitar la utilización de fármacos antipsicóticos y realizar la búsqueda de posibles tumores ocultos.

  18. Anti-allergic, anti-pruritic, and anti-inflammatory activities of Centella asiatica extracts.

    Science.gov (United States)

    George, Mathew; Joseph, Lincy; Ramaswamy

    2009-07-03

    This study investigated antipruritic and anti-inflammatory effect of Centella asiatica extract in rats and anti-allergic in vitro using sheep (Capra hircus) serum method and compound 48/80 induced mast cell degranulation method, compared with standard drug ketotifen fumarate. In rats, extract of Centella asiatica administered orally was examined for anti-pruritic study and chlorpheniramine maleate was used as standard drug while carageenan paw induced inflammatory method was used for the antiinfammatory study. The results show that the extracts of Centella asiatica exhibited antiallergic, anti-pruritic and anti-inflammatory activities.

  19. The Anti-Landscape

    DEFF Research Database (Denmark)

    There have always been some uninhabitable places, but in the last century human beings have produced many more of them. These anti-landscapes have proliferated to include the sandy wastes of what was once the Aral Sea, severely polluted irrigated lands, open pit mines, blighted nuclear zones...... that no longer sustain life. This history includes T. S. Eliot’s Wasteland and Cormac McCarthy’s The Road as well as air pollution, recycled railway lines, photography and landfills. It links theories of aesthetics, politics, tourism, history, geography, and literature into the new synthesis of the environmental...

  20. ELISA cualitativo de IgA anti-Lipopolisacárido de Vibrio cholerae en saliva de humanos

    Directory of Open Access Journals (Sweden)

    Judith Mónica del Campo

    2002-03-01

    Full Text Available Se estandarizó un ELISA para detectar el principal antígeno inductor de protección de Vibrio cholerae en saliva IgA contra el lipopolisacárido (LPS. El estudio se llevó a cabo en voluntarios que fueron inoculados por vía oral con dosis de 0, 107, 108, 109 unidades formadoras de colonias (ufc del candidato vacunal El Tor Ogawa, cepa 638. Las muestras de saliva fueron tomadas de forma seriada, a los 0, 7, 8, 9, 10 y 14 días postinoculación. Se consideró seroconversión si las densidades ópticas eran superiores al nivel de corte y si los incrementos después de inmunizar duplicaban los valores antes de la inmunización. Los resultados, al ser comparados con los grupos experimentales, condición individuos inoculados y los placebos, demostraron que la técnica tiene una sensibilidad del 93,3%, una especificidad del 96,0%, un valor predictivo positivo de 98,2% y negativo de 85,7%, y una eficiencia del 94,1%. Se demostró la presencia de IgA anti LPS en saliva de los individuos inoculados con el candidato vacunal, con una mayor concentración de anticuerpos con el inóculo de (109 ufc y se obtuvo la máxima positividad a los nueve días.

  1. Identification of anti-HBV activities in Paeonia suffruticosa Andr. using GRP78 as a drug target on Herbochip®.

    Science.gov (United States)

    Lam, Iao-Fai; Huang, Min; Chang, Margaret Dah-Tysr; Yao, Pei-Wun; Chou, Yu-Ting; Ng, Sim-Kun; Tsai, Ying-Lin; Lin, Yu-Chang; Zhang, Yun-Feng; Yang, Xiao-Yuan; Lai, Yiu-Kay

    2017-01-01

    Herbochip ® technology is a high throughput drug screening platform in a reverse screening manner, in which potential chemical leads in herbal extracts are immobilized and drug target proteins can be used as probes for screening process [BMC Complementary and Alternative Medicine (2015) 15:146]. While herbal medicines represent an ideal reservoir for drug screenings, here a molecular chaperone GRP78 is demonstrated to serve as a potential target for antiviral drug discovery. We cloned and expressed a truncated but fully functional form of human GRP78 (hGRP78 1-508 ) and used it as a probe for anti-HBV drug screening on herbochips. In vitro cytotoxicity and in vitro anti-HBV activity of the herbal extracts were evaluated by MTT and ELISA assays, respectively. Finally, anti-HBV activity was confirmed by in vivo assay using DHBV DNA levels in DHBV-infected ducklings as a model. Primary screenings using GRP78 on 40 herbochips revealed 11 positives. Four of the positives, namely Dioscorea bulbifera , Lasiosphaera fenzlii , Paeonia suffruticosa and Polygonum cuspidatum were subjected to subsequent assays. None of the above extracts was cytotoxic to AML12 cells, but P. cuspidatum extract (PCE) was found to be cytotoxic to HepG2 2.2.15 cells. Both PCE and P. suffruticosa extract (PSE) suppressed secretion of HBsAg and HBeAg in HepG2 2.2.15 cells. The anti-HBV activity of PSE was further confirmed in vivo. We have demonstrated that GRP78 is a valid probe for anti-HBV drug screening on herbochips. We have also shown that PSE, while being non-cytotoxic, possesses in vitro and in vivo anti-HBV activities. Taken together, our data suggest that PSE may be a potential anti-HBV agent for therapeutic use.

  2. Evaluación de la seroprotección contra sarampión, rubéola y hepatitis B en niños menores de cinco años del Perú, 2011 Evaluation of the seroprotection against measles, rubella and hepatitis B in children under 5 years of age in Peru, 2011

    Directory of Open Access Journals (Sweden)

    Víctor Fiestas Solórzano

    2012-12-01

    Full Text Available Objetivos. Estimar la prevalencia de anticuerpos contra sarampión, rubéola y hepatitis B en niños de 1 a 4 años del Perú. Materiales y métodos. Se realizó una encuesta nacional basada en la aplicación de un cuestionario y obtención de muestra de sangre capilar en papel de filtro para el estudio de anticuerpos contra sarampión, rubéola y hepatitis B en niños de 1 a 4 años. Se utilizó un muestreo probabilístico, estratificado y multietápico con inferencia a nivel nacional y siete ámbitos de estudio: Lima metropolitana, resto de costa urbana, costa rural, sierra urbana, sierra rural, selva urbana y selva rural. Las muestras de sangre capilar fueron procesadas siguiendo protocolos estandarizados para la determinación de anticuerpos mediante técnica de ELISA utilizando reactivos comerciales. Resultados. Se encontró una prevalencia nacional de 91,6% (IC95%: 90,6-92,7%, 91,3% (IC 95%: 90,3-92,4% y 95,9% (IC 95%: 95,0-96,8% para anticuerpos contra sarampión, rubéola y hepatitis B respectivamente. No se evidenció diferencias significativas de las prevalencias entre los diferentes ámbitos de estudio y en los diferentes estratos socioeconómicos de los conglomerados. Conclusiones. En niños de 1 a 4 años se ha estimado una prevalencia nacional de anticuerpos contra sarampión y rubéola entre 90-93%, mientras que para anticuerpos contra hepatitis B (anti-HBsAg entre 95-97%.Objectives. To estimate the prevalence of antibodies against measles, rubella and hepatitis B in children aged between 1 and 4 years in Peru. Materials and methods. A national survey was conducted based on a questionnaire and capillary blood sample taken on filter paper in order to study antibodies against measles, rubella and hepatitis B in children from 1 to 4 years of age. A stratified, multistage, probability sampling design was used to be representative at the national level and at level of seven ambits, including the Metropolitan Lima Area, the rest of the

  3. Comparison of an enzyme-immunoassay with a radio-immunoassay ...

    African Journals Online (AJOL)

    1990-07-21

    Jul 21, 1990 ... The anti-HBs test uses a 'sandwich' method in which the patient's serum is added to beads coated with HBsAg and, after a period of incubation, HBsAg conjugated with iodine-. 125 which binds to any anti-HBs on the bead, is added. Department of Virology, University of the Witwatersrand and National ...

  4. Anti-vibration gloves?

    Science.gov (United States)

    Hewitt, Sue; Dong, Ren G; Welcome, Daniel E; McDowell, Thomas W

    2015-03-01

    For exposure to hand-transmitted vibration (HTV), personal protective equipment is sold in the form of anti-vibration (AV) gloves, but it remains unclear how much these gloves actually reduce vibration exposure or prevent the development of hand-arm vibration syndrome in the workplace. This commentary describes some of the issues that surround the classification of AV gloves, the assessment of their effectiveness and their applicability in the workplace. The available information shows that AV gloves are unreliable as devices for controlling HTV exposures. Other means of vibration control, such as using alternative production techniques, low-vibration machinery, routine preventative maintenance regimes, and controlling exposure durations are far more likely to deliver effective vibration reductions and should be implemented. Furthermore, AV gloves may introduce some adverse effects such as increasing grip force and reducing manual dexterity. Therefore, one should balance the benefits of AV gloves and their potential adverse effects if their use is considered. © Crown copyright 2014.

  5. Anti-sulfotyrosine antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Bertozzi, Carolyn R [Berkeley, CA; Kehoe, John [Saint Davids, PA; Bradbury, Andrew M [Santa Fe, NM

    2009-09-15

    The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

  6. anti p and anti Λ production in Si+Au collisions at the AGS

    International Nuclear Information System (INIS)

    Wu, Yuedong

    1996-01-01

    Anti p and anti Λ production in central Si + Au collisions has been measured by E589 at the BNL-AGS. Preliminary m perpendicular spectra are presented for anti p's and anti Λ's. The dn/dy distribution for anti p's is also presented. Based on the anti p and anti Λ measurements, anti Λ/anti p ratios are calculated in the rapidity range of 1.1-1.5

  7. Calculations of anti pp → anti yy near threshold

    International Nuclear Information System (INIS)

    Eisenstein, R.A.

    1982-01-01

    The reactions anti pp → anti yy will soon be studied near threshold at LEAR. Comparison of theoretical calculations with the measured differential cross sections, polarizations and spin correlations may help to determine the role of various meson exchange terms and the extent to which quark degrees of freedom are important. Calculations along these lines are in progress and are described briefly

  8. Reviews Evaluating the Anti-Plasmodium, Anti-Pyretic and ...

    African Journals Online (AJOL)

    Discovery and Innovation ... With the introduction of cheap anti-malaria drugs, there was marked reduction in the use of local herbal medicine. ... Key Words: medicinal plants, local traditional medicine, malaria, anti-malarial properties, antipyretic and analgesic properties, concoctions, efficacy, resistance, generic ...

  9. Anti-Hyperglycemic And Anti-Hyperlipidemic Potential Of A ...

    African Journals Online (AJOL)

    Conclusion: Our study suggests that diabegon could be included as a preventive treatment in metabolic syndrome subjects with type 2 diabetes especially for long term treatment as it efficiently shows anti-hyperglycemic and anti-lipidemic effects with no adverse impacts on the liver and kidney. Key words: Metabolic ...

  10. Anticorpos contra LDL-ox e síndrome coronariana aguda Anticuerpos contra LDL-ox y síndrome coronario agudo Antibodies against OxLDL and acute coronary syndrome

    Directory of Open Access Journals (Sweden)

    Ana Maria Brito Medeiros

    2010-07-01

    Full Text Available FUNDAMENTO: A oxidação da lipoproteína de baixa densidade (LDL-ox induz à formação de epítopos imunogênicos na molécula. A presença de autoanticorpos contra a LDL-ox tem sido demonstrada no soro de pacientes com doença arterial coronariana (DAC. Contudo, o papel desses autoanticorpos na fisiopatologia das síndromes coronarianas agudas (SCA e o seu significado clínico permanecem indefinidos. OBJETIVO: Avaliar a associação entre autoanticorpos contra a LDL-ox e SCA. MÉTODOS: Os títulos de imunoglobulina G autoanticorpos contra a LDL-ox por cobre (antiLDL-ox e contra o peptídeo sintético D derivado da apolipoproteína B (antipeptD foram determinados por ensaio imunoenzimático (ELISA em 90 pacientes, nas primeiras 12h de SCA (casos e em 90 pacientes com DAC crônica (controles. RESULTADOS: Os resultados mostraram que os títulos de antiLDL-ox foram significativamente mais elevados (p = 0,017 nos casos (0,40 ± 0,22, do que nos controles (0,33 ± 0,23. Por outro lado, os títulos de antipeptD foram significativamente menores (p FUNDAMENTO: La oxidación de la lipoproteína de baja densidad (LDL-ox induce a la formación de epítopos inmunogénicos en la molécula. La presencia de autoanticuerpos contra la LDL-ox ha sido demostrada en el suero de pacientes con enfermedad arterial coronaria (EAC. No obstante eso, el papel de esos autoanticuerpos en la fisiopatología de los síndromes coronarios agudos (SCA y su significado clínico permanecen indefinidos. OBJETIVO: Evaluar la asociación entre autoanticuerpos contra la LDL-ox y SCA. MÉTODOS: Los títulos de inmunoglobulina G autoanticuerpos contra la LDL-ox por cobre (antiLDL-ox y contra el péptido sintético D derivado de la apolipoproteína B (antipeptD fueron determinados por ensayo inmunoenzimático (ELISA en 90 pacientes, en las primeras 12h de SCA (casos y en 90 pacientes con EAC crónica (controles. RESULTADOS: Los resultados mostraron que los títulos de antiLDL-ox fueron

  11. Television and Anti-Racism.

    Science.gov (United States)

    Ali, Yasmin

    The "new" anti-racists of the 1980s in contemporary Britain consider racism, particularly against blacks, as both a structural and a white problem. Anti-racist activists, whose attitude is a mirror-image of the political left's general concern about the media--where blacks are in the minority--regard the media, especially television, as…

  12. Effect on HBs antigen clearance of addition of pegylated interferon alfa-2a to nucleos(t)ide analogue therapy versus nucleos(t)ide analogue therapy alone in patients with HBe antigen-negative chronic hepatitis B and sustained undetectable plasma hepatitis B virus DNA: a randomised, controlled, open-label trial.

    Science.gov (United States)

    Bourlière, Marc; Rabiega, Pascaline; Ganne-Carrie, Nathalie; Serfaty, Lawrence; Marcellin, Patrick; Barthe, Yoann; Thabut, Dominique; Guyader, Dominique; Hezode, Christophe; Picon, Magali; Causse, Xavier; Leroy, Vincent; Bronowicki, Jean Pierre; Carrieri, Patrizia; Riachi, Ghassan; Rosa, Isabelle; Attali, Pierre; Molina, Jean Michel; Bacq, Yannick; Tran, Albert; Grangé, Jean Didier; Zoulim, Fabien; Fontaine, Hélène; Alric, Laurent; Bertucci, Inga; Bouvier-Alias, Magali; Carrat, Fabrice

    2017-03-01

    Findings from uncontrolled studies suggest that addition of pegylated interferon in patients with HBe antigen (HBeAg)-negative chronic hepatitis B receiving nucleos(t)ide analogues with undetectable plasma hepatitis B virus (HBV) DNA might increase HBs antigen (HBsAg) clearance. We aimed to assess this strategy. In this randomised, controlled, open-label trial, we enrolled patients aged 18-75 years with HBeAg-negative chronic hepatitis B and documented negative HBV DNA while on stable nucleos(t)ide analogue regimens for at least 1 year from 30 hepatology tertiary care wards in France. Patients had to have an alanine aminotransferase concentration of less than or equal to five times the upper normal range, no hepatocellular carcinoma, and a serum α fetoprotein concentration of less than 50 ng/mL, normal dilated fundus oculi examination, and a negative pregnancy test in women. Patients with contraindications to pegylated interferon were not eligible. A centralised randomisation used computer-generated lists of random permuted blocks of four with stratification by HBsAg titres (sida et les hépatites virales (France Recherche Nord&sud Sida-vih Hepatites). Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Charmed meson rescattering in the reaction anti pd→ anti DDN

    International Nuclear Information System (INIS)

    Haidenbauer, J.; Krein, G.; Meissner, U.G.; Sibirtsev, A.

    2008-01-01

    We examine the possibility to extract information about the DN and anti DN interactions from the anti pd→D 0 D - p reaction. We utilize the notion that the open-charm mesons are first produced in the annihilation of the antiproton on one nucleon in the deuteron and subsequently rescatter on the other (the spectator) nucleon. The latter process is then exploited for investigating the DN and anti DN interactions. We study different methods for isolating the contributions from the D 0 p and D - p rescattering terms. (orig.)

  14. Anti-nuclear movements

    International Nuclear Information System (INIS)

    Ruedig, W.

    1990-01-01

    Nuclear power, heralded in the years after World War II as the answer to the world's energy needs, has in more recent times become the focus of intense ecological, political and economic debate. In this study, the current worldwide opposition to nuclear power is examined from its origins in expert dissent to the widespread development of grassroots activity. Chapter headings include: Social Movements: A Theoretical Framework; Creating the Preconditions for Public Protest; Local and Regional Opposition: Mobilizing the Grass Roots; Local Opposition and the Politicization of Nuclear Power; The Use of Local Opposition as a Political Resource; Local Opposition and Social Movement Analysis; The Removal of Political Stimuli: The Unpolitics of Nuclear Siting; Analyzing Host Community Attitudes: The Survey Evidence; Attitudes and Political Action of Nuclear Host Communities: Approaches and Explanations; Novel Siting Approaches and their Political Implications; Siting and Social Movement Analysis; Patterns and Outcomes of Nuclear Energy Conflicts; The Future of the Nuclear Energy Conflict. Throughout the text, analysis and theory are blended with detailed accounts of the growth and activities of individual anti-nuclear organizations in different countries. (author)

  15. Anti-diarrheal, anti-secretory, anti-spasmodic and antiulcer activities of

    African Journals Online (AJOL)

    In isolated tissue (rabbit jejunum), Am.Cr concentration-dependently (0.01 - 3.0 mg/mL) produced relaxation of K+ (80 mM)-induced and spontaneous contractions at ... anti-secretory, antispasmodic and anti-ulcer activities, probably mediated through dual mechanisms, including Ca2+ influx and PDE enzyme(s) inhibition.

  16. Anti-allergic, anti-pruritic, and anti-inflammatory activities of Centella ...

    African Journals Online (AJOL)

    This study investigated antipruritic and anti-inflammatory effect of Centella asiatica extract in rats and anti-allergic in vitro using sheep (Capra hircus) serum method and compound 48/80 induced mast cell degranulation method, compared with standard drug ketotifen fumarate. In rats, extract of Centella asiatica administered ...

  17. Impact of the timing of hepatitis B virus identification and anti-hepatitis B virus therapy initiation on the risk of adverse liver outcomes for patients receiving cancer therapy.

    Science.gov (United States)

    Hwang, Jessica P; Suarez-Almazor, Maria E; Cantor, Scott B; Barbo, Andrea; Lin, Heather Y; Ahmed, Sairah; Chavez-MacGregor, Mariana; Donato-Santana, Christian; Eng, Cathy; Ferrajoli, Alessandra; Fisch, Michael J; McLaughlin, Peter; Simon, George R; Rondon, Gabriela; Shpall, Elizabeth J; Lok, Anna S

    2017-09-01

    Data on the incidence of adverse liver outcomes are limited for cancer patients with chronic (hepatitis B surface antigen [HBsAg]-positive/hepatitis B core antibody [anti-HBc]-positive) or past (HBsAg-negative/anti-HBc-positive) hepatitis B virus (HBV) after chemotherapy. This study was aimed at determining the impact of test timing and anti-HBV therapy on adverse liver outcomes in these patients. Patients with solid or hematologic malignancies who received chemotherapy between 2004 and 2011 were retrospectively studied. HBV testing and anti-HBV therapy were defined as early at the initiation of cancer therapy and as late after initiation. Outcomes included hepatitis flares, hepatic impairment, liver failure, and death. Time-to-event analysis was used to determine incidence, and multivariate hazard models were used to determine predictors of outcomes. There were 18,688 study patients (80.4% with solid tumors). The prevalence of chronic HBV was 1.1% (52 of 4905), and the prevalence of past HBV was 7.1% (350 of 4905). Among patients with solid tumors, late identification of chronic HBV was associated with a higher risk of hepatitis flare (hazard ratio [HR], 4.02; 95% confidence interval [CI], 1.26-12.86), hepatic impairment (HR, 8.48; 95% CI, 1.86-38.66), liver failure (HR, 9.38; 95% CI, 1.50-58.86), and death (HR, 3.90; 95% CI, 1.19-12.83) in comparison with early identification. Among patients with hematologic malignancies and chronic HBV, the risk of death was 7.8 (95% CI, 1.73-35.27) times higher for persons with late initiation of anti-HBV therapy versus early initiation. Patients with late identification of chronic HBV had late or no anti-HBV therapy. Chronic HBV predicted liver failure in patients with solid or hematologic malignancies, whereas male sex and late identification were predictors for patients with solid tumors. Early identification correlates with early anti-HBV therapy and reduces the risk of liver failure and death in chronic HBV patients

  18. What is it really? Anti-G or Anti-D plus Anti-C: Clinical Significance in Antenatal Mothers.

    Science.gov (United States)

    Das, Soumya; Shastry, Shamee; Murugesan, M; B, Poornima Baliga; Shastry, Shamee

    2017-06-01

    G antigen of Rh blood group system is present either along with D and/or C positive red cells. Hence, [serologically anti-G presents with the similar picture as that of multiple antibodies (anti-D + anti-C). Differentiating them is important as anti-D + anti-C causes severe hemolytic disease of the fetus and newborn than anti-G. In pregnancies with anti-G alone, alloimmunization due to D antigen could be prevented by prophylactic administration of RhIg. Differentiating between anti-D + C from anti-G in alloimmunized pregnant mothers becomes essential. Sera from antenatal mothers, whose antibody identification by 11-cell panel gave a pattern for anti-D and anti-C were selected. Extended phenotyping for Rh system was performed for these antenatal cases. Differential adsorption and elution testing using R 2 R 2 cells initially and r'r cells subsequently were performed to distinguish anit-G from anti-D + anti-C. Antibody titers of these antibodies were determined and their clinical outcome in the newborn was followed. A pattern suggestive of anti D and anti C on antibody identification were observed in six antenatal cases. On further workup 50 % of them confirmed to have anti G. Antibody titers of anti-G and anti-C were lower than that of Anti-D. All newborns were sensitized in vivo and the antibody specificity in them were confirmed with elution studies. The mothers who had only anti-G were subsequently administered with an appropriate dose of RhIg.Differential adsorption and elution studies help in identifying anti-G and distinguishing it from anti-D plus anti-C, thus helping in better patient management.

  19. The anti pp yields anti λ λ reaction near threshold

    International Nuclear Information System (INIS)

    Maher, C.J.

    1986-06-01

    Measurements of differential and integrated cross sections as well as final state polarizations for the anti p p → anti Λ Λ reaction are presented. The reaction was studied at two incident antiproton momenta (1476.5 MeV/c and 1507.5 MeV/c) corresponding to total center of mass energies of 15.5 MeV and 26.4 MeV above the reaction threshold. The trajectories of charged decay products of the anti Λ and Λ were observed in a multiwire proportional chamber and in two sets of drift chambers. The data were analyzed with a computer program which reconstructed anti p p → anti Λ Λ → anti p π + pπ - events and performed kinematic fitting. The results are compared to several recent meson exchange calculations, and a one-gluon exchange calculation. The experiment was performed at the Low Energy Antiproton Ring (LEAR) at CERN. The data presented represent the first results of the PS185 collaboration's study of the threshold production of hyperon-antihyperon states

  20. Factores bióticos y abióticos que determinan la seroprevalencia de anticuerpos contra Trypanosoma Cruzi en el municipio de Palmar de Bravo, Puebla, México Biotic and abiotic determinants of seroprevalence of antibodies against Trypanosoma cruzi in Palmar de Bravo, Puebla, Mexico

    Directory of Open Access Journals (Sweden)

    Francisca Sosa-Jurado

    2004-02-01

    Full Text Available OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi y su relación con los factores bióticos y abióticos en Palmar de Bravo, Puebla, México. MATERIAL Y MÉTODOS: Estudio transversal efectuado en agosto de 2000 a septiembre de 2001, con una muestra aleatoria simple de 390 voluntarios residentes en Palmar de Bravo, Puebla, México. Se hizo determinación de anticuerpos contra T cruzi con técnicas serológicas validadas, búsqueda del vector y de reservorios domésticos, así como determinación de asociación entre caso positivo y factores de riesgo bióticos y abióticos. El análisis estadístico consistió en índice Kappa para las pruebas diagnósticas, empleando tabla de contingencia de 2 x 2; ji cuadrada corregida de Yates, exacta de Fisher y la razón de posibilidad para estimar la significancia de la asociación de factores bióticos y abióticos. RESULTADOS: La seroprevalencia fue de 4% en la población humana estudiada y de los reservorios (equinos, porcinos y caninos, sólo 10% de los caninos resultaron reactivos. Los vectores identificados fueron T barberi y T pallidipennis, con índice de dispersión e índice de colonización de 55 y 40%, respectivamente. Los factores de riesgo más importantes fueron la altitud (>2 150 y OBJECTIVE: To establish the relationship between seroprevalence for antibodies against Trypanosoma cruzi and its relationship with biotic and abiotic factors. MATERIAL AND METHODS: A cross-sectional study was conducted between August 2000 and September 2001. The study population consisted of a simple random sample of 390 volunteers residing in Palmar de Bravo, Puebla, Mexico. Sample and data collection procedures included assaying antibodies against T cruzi with validated assays, and searching for domestic reservoirs and triatomine bugs. The relationship between biotic and abiotic factors with seropositivity was assessed. Statistical analysis was conducted using Kappa values for

  1. Online Anti-Brand Herds

    DEFF Research Database (Denmark)

    Langley, David J.; Tan, Chee-Wee; Worm, Daniël

    The online environment offers a fertile breeding ground for anti-brand herds of disgruntled consumers. Firms are often caught off guard by the unpredictability of such herds and, as a consequence, are forced into a reactive, defensive stance. We conduct a social media analysis that aims to shed...... light on the formation, growth, and dissolution of online anti-brand herds. First we expand on the concept of environmental turbulence to advance core properties unique to online herd behavior. Next, based on evidence gathered from 40 online anti-brand herd episodes targeting two prominent firms from...... the Netherlands, we develop an analytical model to investigate drivers of herd formation, growth, and dissolution. Finally, combining environmental turbulence literature with our empirical findings, we derive a novel typology of online anti-brand herd behaviors, and put forward six propositions to guide theory...

  2. Anti-C.Diff Pill

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Anand [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2018-01-30

    The anti- C.diff pill is being developed for patients who are at high risk for Clostridium difficile, a bacterium that causes diarrhea and serious intestinal conditions like colitis. C-diff is deadly, killing over 30,000 people a year in the United States and costing an average of $42,000 per treatment. The most common treatment is a fecal transplant (FT). The anti- C.diff replaces the invasive and messy FT practice with a pill.

  3. Seroprevalencia de anticuerpos contra Trypanosoma cruzi y su asociación con factores de riesgo en menores de 18 años de Veracruz, México Seroprevalence of Trypanosoma cruzi antibodies and associated risk factors among the population under 18 years of age in Veracruz, Mexico

    Directory of Open Access Journals (Sweden)

    Paz María Salazar

    2007-08-01

    Full Text Available OBJETIVOS: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en menores de 18 años del estado de Veracruz, México, e identificar los factores de riesgo asociados con las características de la vivienda y la presencia y distribución del agente transmisor. MÉTODOS: Estudio epidemiológico transversal en la población menor de 18 años que vivía en 10 de las 11 jurisdicciones sanitarias del estado de Veracruz, México, entre 2000 y 2001. La presencia de anticuerpos contra T. cruzi se determinó mediante una prueba de hemaglutinación indirecta (HAI y un ensayo inmunoenzimático indirecto tipo ELISA en muestras de suero tomadas en papel de filtro y se confirmaron mediante las pruebas de HAI, ELISA e inmunofluorescencia indirecta (IFI en muestras de suero. Se aplicó un cuestionario para evaluar las condiciones de la vivienda y se calcularon los índices entomológicos de triatominos intra y peridomiciliarios. Los resultados se evaluaron mediante análisis bifactorial y multifactorial por regresión logística no condicional. RESULTADOS: De las 150 personas inicialmente reactivas, 14 resultaron positivas (5 mediante la prueba de HAI, ELISA e IFI; 6 por HAI y ELISA y 3 por ELISA e IFI, para una prevalencia general de 0,91% (IC95%: 0,85% a 0,94%. Los casos positivos residían en cinco jurisdicciones sanitarias y la mayor prevalencia se encontró en Tuxpan: 5,2% (IC95%: 1,2% a 9,0%. Los factores de riesgo fueron el haber visto chinches dentro de la vivienda y los techos con fisuras. La única especie del agente transmisor capturada fue Triatoma dimidiata. Los índices entomológicos de infestación, colonización e infección natural fueron: 10,9%, 50,0% y 9,0%, respectivamente. CONCLUSIONES: Se confirmó la transmisión vectorial activa de la infección en el estado de Veracruz, México, con una seroprevalencia general de anticuerpos contra T. cruzi en menores de 18 años de 0,91%. Se debe seguir atentamente la seroprevalencia en

  4. Approach to a Pregnant Woman with Anti D + Anti C Reactivity Pattern: A Diagnostic Conundrum.

    Science.gov (United States)

    Rai, Preeti; Sharma, Geetika; Singh, Deeksha; Garg, Jyoti

    2017-09-01

    The Rhesus G antigen is present on all RBCs that are C+ and also on most D+ RBCs. Due to this co-distribution of G with either C or D antigen, it mimics a reactivity pattern of anti C + anti D on Indirect Antiglobulin Test (IAT), though the role of Anti G in causing Hemolytic Disease of Newborn (HDN) is controversial. The differentiation of anti D, anti C, and anti G is essential particularly in pregnant females. We hereby report a rare case of anti G with anti D and anti C in a pregnant woman with emphasis on approach to identify anti D+C+G and its implications.

  5. Performance evaluation of four dominant anti-hepatitis B core antigen (HBcAb) kits in Japan for preventing de novo hepatitis B virus (HBV) infection.

    Science.gov (United States)

    Kobayashi, Eiji; Deguchi, Matsuo; Kagita, Masanori; Yoshioka, Nori; Kita, Mifumi; Asari, Seishi; Suehisa, Etsuji; Hidaka, Yoh; Iwatani, Yoshinori

    2015-01-01

    The determination of antibody to hepatitis B core antigen (HBcAb) has become an important means of evaluating the risk factors of de novo hepatitis B virus (HBV) infection before starting intensive immunosuppressive drug therapies. Four dominant HBcAb determination reagents used in Japan were evaluated with HBcIgM, HBsAg, HBsAb, HBeAb, and HBV DNA reagents in order to study their clinical utility. Four kinds of HBcAb reagent kits (HBcAb Total and HBcAb-IgG reagent) were evaluated with 526 clinical specimens, including 344 negative specimens, at Osaka University Hospital. The dynamic range of each kit was evaluated by testing serially diluted serum from pooled sera with high HBcAb concentration. The reagent that showed the largest dynamic range was the Lumipulse HBcAb-N (HBcAb-IgG reagent). Regarding clinical sensitivity and specificity, Centaur HBcAb (HBcAb Total reagent) gave several "doubtful negative" results and ARCHITECT HBcII (HBcAb Total reagent) had the most discrepant positive results. By comparing the cut-off-index distribution of negative specimens using a parameter of "distance from the mean to the cut-off divided by the SD", Centaur was determined to be the best (distance/SD = 12.65), with Lumipulse and Elecsys Anti-HBc (HBcAb Total reagent) in the second group (8.13 and 7.00, respectively), and ARCHITECT rated as the worst (3.25). In this evaluation, Elecsys and Lumipulse HBcAb kits showed good clinical sensitivity and specificity and were considered to be suitable for evaluating the risk factors of de novo HBV infection.

  6. Persistent risk of HBV reactivation despite extensive lamivudine prophylaxis in haematopoietic stem cell transplant recipients who are anti-HBc-positive or HBV-negative recipients with an anti-HBc-positive donor.

    Science.gov (United States)

    Cerva, C; Colagrossi, L; Maffongelli, G; Salpini, R; Di Carlo, D; Malagnino, V; Battisti, A; Ricciardi, A; Pollicita, M; Bianchi, A; Picardi, A; Cudillo, L; Cerretti, R; De Angelis, G; Cantonetti, M; Andreoni, M; Perno, C F; Arcese, W; Svicher, V; Sarmati, L

    2016-11-01

    The overall rate of hepatitis B virus (HBV) reactivation was evaluated in a population of 373 haematological stem cell transplant (HSCT) patients treated with lamivudine (LMV) if they were anti-HBc-positive/HBV-DNA-negative recipients or if they were HBV-negative recipients with an anti-HBc-positive donor. The incidence of HBV reactivation was calculated in two groups of autologous (auto) or allogeneic (allo) HSCT patients who were stratified according to their HBV serostatus. The former group included 57 cases: 10 auto-HSCT and 27 allo-HSCT anti-HBc-positive recipients, two auto-HSCT and three allo-HSCT inactive carriers, and 15 allo-HSCT recipients with an anti-HBc-positive donor. Forty-seven (82.4%) patients in this group received LMV prophylaxis (the median (interquartile range, IQR) of LMV treatment was 30 (20-38) months). The second group consisted of 320 anti-HBc-negative auto-HSCT and allo-HSCT recipients with anti-HBc-negative donors. None of these patients received any prophylaxis. Two patients in the first group and two in the second group experienced reactivation of HBV infection, with an incidence of 3.5% (95% CI 0.4-12.1%) and 0.6% (95% CI 0.1-2.2%), respectively. Only one out of four reactivated patients was LMV-treated. The cumulative probability of HBV reactivation at 6 years from HSCT was 15.8% (95% CI 15.2-16.4%). Three of four viral isolates obtained from the HBV-reactivated patients harboured mutations in the immune-active HBsAg-region. In a HSCT population carefully evaluated for HBV prophylaxis, a risk of HBV reactivation persisted in the group of patients who were not LMV-treated. Only one LMV-treated patient experienced reactivation of HBV with a resistant HBV isolate. Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  7. Approach to a Pregnant Woman with Anti D + Anti C Reactivity Pattern: A Diagnostic Conundrum

    OpenAIRE

    Rai, Preeti; Sharma, Geetika; Singh, Deeksha; Garg, Jyoti

    2017-01-01

    The Rhesus G antigen is present on all RBCs that are C+ and also on most D+ RBCs. Due to this co-distribution of G with either C or D antigen, it mimics a reactivity pattern of anti C + anti D on Indirect Antiglobulin Test (IAT), though the role of Anti G in causing Hemolytic Disease of Newborn (HDN) is controversial. The differentiation of anti D, anti C, and anti G is essential particularly in pregnant females. We hereby report a rare case of anti G with anti D and anti C in a pregnant woma...

  8. Anti-regulatory T cells

    DEFF Research Database (Denmark)

    Andersen, Mads Hald

    2017-01-01

    Our initial understanding of immune-regulatory cells was based on the discovery of suppressor cells that assure peripheral T-cell tolerance and promote immune homeostasis. Research has particularly focused on the importance of regulatory T cells (Tregs) for immune modulation, e.g. directing host...... responses to tumours or inhibiting autoimmunity development. However, recent studies report the discovery of self-reactive pro-inflammatory T cells—termed anti-regulatory T cells (anti-Tregs)—that target immune-suppressive cells. Thus, regulatory cells can now be defined as both cells that suppress immune...... reactions as well as effector cells that counteract the effects of suppressor cells and support immune reactions. Self-reactive anti-Tregs have been described that specifically recognize human leukocyte antigen-restricted epitopes derived from proteins that are normally expressed by regulatory immune cells...

  9. Anti-G with concomitant anti-C and anti-D: A case report in a pregnant woman

    Directory of Open Access Journals (Sweden)

    Rabeya Yousuf

    2017-01-01

    Full Text Available The G antigen of Rh blood group system is present in almost all D-positive or C-positive red cells but absent from red cells lacking D and C antigens. The differentiation of anti-D and anti-C from anti-G is not necessary for routine transfusion; however, during pregnancy, it is important because anti-G can masquerade as anti-D and anti-C with initial antibody testing. The false presence of anti-D will exclude the patient from receiving anti-D immunoglobulin (RhIG when the patient actually is a candidate for RhIG prophylaxis. Moreover, patients with positive anti-D or anti-G are at risk of developing hemolytic disease of the fetus and newborn and need close monitoring. Thus, proper identification allows the clinicians to manage patients properly. This case report highlights a rare case of anti-G together with anti-D and anti-C in a pregnant woman. This report disseminates knowledge on identification of anti-G and its importance in pregnant women.

  10. Anti-G with concomitant anti-C and anti-D: A case report in a pregnant woman.

    Science.gov (United States)

    Yousuf, Rabeya; Mustafa, Ahmad Nasirudin; Ho, Siew-Ling; Tang, Yee-Loong; Leong, Chooi-Fun

    2017-01-01

    The G antigen of Rh blood group system is present in almost all D-positive or C-positive red cells but absent from red cells lacking D and C antigens. The differentiation of anti-D and anti-C from anti-G is not necessary for routine transfusion; however, during pregnancy, it is important because anti-G can masquerade as anti-D and anti-C with initial antibody testing. The false presence of anti-D will exclude the patient from receiving anti-D immunoglobulin (RhIG) when the patient actually is a candidate for RhIG prophylaxis. Moreover, patients with positive anti-D or anti-G are at risk of developing hemolytic disease of the fetus and newborn and need close monitoring. Thus, proper identification allows the clinicians to manage patients properly. This case report highlights a rare case of anti-G together with anti-D and anti-C in a pregnant woman. This report disseminates knowledge on identification of anti-G and its importance in pregnant women.

  11. Anti-inflammatory, Anti-estrogenic, and Anti-implantation Activity of Bergia suffruticosa (Delile) Fenzl

    Science.gov (United States)

    Bind, Sandeep Kumar; Jivrajani, Mehul; Anandjiwala, Sheetal; Nivsarkar, Manish

    2015-01-01

    Background: Bergia suffruticosa (Delile) Fenzl (Syn. Bergia odorata Edgew) (Elatinaceae family) is used traditionally to repair bones and is applied as a poultice on sores. It is also used for stomach troubles and as an antidote to scorpion stings. So far, very little scientific work has been reported to validate its ethnomedical uses in the alleviation of pain, bone repair, etc., Objective: This study was designed to explore the anti-inflammatory and anti-implantation potential of n-hexane extract of B. suffruticosa whole plant in mice along with identification of its chemical constituents. Materials and Methods: n-Hexane extract of B. suffruticosa whole plant was screened for acute and chronic anti-inflammatory activity followed by an anti-estrogenic activity. Eventually, n-hexane extract was tested for anti-implantation activity by exploiting markers of uterine receptivity, lipid peroxidation, and superoxide enzyme activity. The extract was administered orally at a dose of 100 mg/kg body weight in each study. Results: Thin layer chromatography fingerprint profile of n-hexane extract revealed the presence of lupeol and β-sitosterol. The n-hexane extract reduced the edema by 80% in acute inflammation, whereas it reduced edema to 75% on the 5th day in chronic inflammation. The n-hexane extract reduced elevated malonaldehyde level from 6 to 2.5 nmol/g × 10−5 and increased superoxide dismutase enzyme activity from 0 to 350 units/g in treated animals on the 5th day of pregnancy. Moreover, extract decreased uterine weight from 0.33 to 0.2 g in estradiol treated animals. Conclusion: These results indicate that n-hexane extract of B. suffruticosa is having potent anti-inflammatory, anti-estrogenic, and anti-implantation activity. This is the first report of all the pharmacological activities of B. suffruticosa mentioned above. SUMMARY TLC fingerprint profile of n-hexane extract of Bergia suffruticosa whole plant revealed the presence of lupeol and

  12. Antioxidant, anti-inflammatory and anti-nociceptive properties of ...

    African Journals Online (AJOL)

    The anti-inflammatory potential was investigated in egg-albumin and carrageenan induced paw edema models while the antinociceptive activity was determined using acetic acid induced writhing reflex and tail immersion tests at the doses of 50, 100 and 200 mg/kg. The negative control group (group A) received distilled ...

  13. Selective anti-cancer agents as anti-aging drugs.

    Science.gov (United States)

    Blagosklonny, Mikhail V

    2013-12-01

    Recent groundbreaking discoveries have revealed that IGF-1, Ras, MEK, AMPK, TSC1/2, FOXO, PI3K, mTOR, S6K, and NFκB are involved in the aging process. This is remarkable because the same signaling molecules, oncoproteins and tumor suppressors, are well-known targets for cancer therapy. Furthermore, anti-cancer drugs aimed at some of these targets have been already developed. This arsenal could be potentially employed for anti-aging interventions (given that similar signaling molecules are involved in both cancer and aging). In cancer, intrinsic and acquired resistance, tumor heterogeneity, adaptation, and genetic instability of cancer cells all hinder cancer-directed therapy. But for anti-aging applications, these hurdles are irrelevant. For example, since anti-aging interventions should be aimed at normal postmitotic cells, no selection for resistance is expected. At low doses, certain agents may decelerate aging and age-related diseases. Importantly, deceleration of aging can in turn postpone cancer, which is an age-related disease.

  14. Evaluation of the anti-inflammatory Evaluation of the Anti ...

    African Journals Online (AJOL)

    The anti-inflammatory activity of the aqueous leaf extract of Pterocarpus santalinoides was evaluated using the carrageenan-induced paw oedema and leucocyte migration in rats, and croton oil-induced ear oedema in mice. The extract (50-100mg/kg) and indomethacin (10mg/kg) produced significant (p<0.05) inhibitions of ...

  15. Synthesis, Anti-inflammatory and Anti-nociceptive Evaluation of ...

    African Journals Online (AJOL)

    The final products were purified on column chromatography, eluting with ... the reference drug. Results: The compounds were obtained in high yield (70 – 90 %) and purity. The anti-inflammatory results showed a poor activity for the compounds except o-palmitoylamino ..... causes an increase in the peritoneal fluid level of.

  16. Anti-inflammatory, anti-nociceptive and antipyretic potential of ...

    African Journals Online (AJOL)

    Materials and Methods: Extracts of Terminalia citrina fruits were evaluated at doses of 200mg/kg, 400mg/kg and 600mg/kg in albino mice for preventive effect in inflammatory edema, peripheral pain sensation and pyrexia. Carrageenan induced paw edema method was utilized to evaluate anti-inflammatory activity.

  17. Anti-Oxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Resveratrol in Ocular Diseases.

    Science.gov (United States)

    Lançon, Allan; Frazzi, Raffaele; Latruffe, Norbert

    2016-03-02

    Resveratrol (3,4',5 trihydroxy-trans-stilbene) is one of the best known phytophenols with pleiotropic properties. It is a phytoalexin produced by vine and it leads to the stimulation of natural plant defenses but also exhibits many beneficial effects in animals and humans by acting on a wide range of organs and tissues. These include the prevention of cardiovascular diseases, anti-cancer potential, neuroprotective effects, homeostasia maintenance, aging delay and a decrease in inflammation. Age-related macular degeneration (AMD) is one of the main causes of deterioration of vision in adults in developed countries This review deals with resveratrol and ophthalmology by focusing on the antioxidant, anti-inflammatory, and anti-angiogenic effects of this molecule. The literature reports that resveratrol is able to act on various cell types of the eye by increasing the level of natural antioxidant enzymatic and molecular defenses. Resveratrol anti-inflammatory effects are due to its capacity to limit the expression of pro-inflammatory factors, such as interleukins and prostaglandins, and also to decrease the chemo-attraction and recruitment of immune cells to the inflammatory site. In addition to this, resveratrol was shown to possess anti-VEGF effects and to inhibit the proliferation and migration of vascular endothelial cells. Resveratrol has the potential to be used in a range of human ocular diseases and conditions, based on animal models and in vitro experiments.

  18. Anti-Oxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Resveratrol in Ocular Diseases

    Directory of Open Access Journals (Sweden)

    Allan Lançon

    2016-03-01

    Full Text Available Resveratrol (3,4′,5 trihydroxy-trans-stilbene is one of the best known phytophenols with pleiotropic properties. It is a phytoalexin produced by vine and it leads to the stimulation of natural plant defenses but also exhibits many beneficial effects in animals and humans by acting on a wide range of organs and tissues. These include the prevention of cardiovascular diseases, anti-cancer potential, neuroprotective effects, homeostasia maintenance, aging delay and a decrease in inflammation. Age-related macular degeneration (AMD is one of the main causes of deterioration of vision in adults in developed countries This review deals with resveratrol and ophthalmology by focusing on the antioxidant, anti-inflammatory, and anti-angiogenic effects of this molecule. The literature reports that resveratrol is able to act on various cell types of the eye by increasing the level of natural antioxidant enzymatic and molecular defenses. Resveratrol anti-inflammatory effects are due to its capacity to limit the expression of pro-inflammatory factors, such as interleukins and prostaglandins, and also to decrease the chemo-attraction and recruitment of immune cells to the inflammatory site. In addition to this, resveratrol was shown to possess anti-VEGF effects and to inhibit the proliferation and migration of vascular endothelial cells. Resveratrol has the potential to be used in a range of human ocular diseases and conditions, based on animal models and in vitro experiments.

  19. Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties.

    Science.gov (United States)

    Lee, Jin-Ching; Hou, Ming-Feng; Huang, Hurng-Wern; Chang, Fang-Rong; Yeh, Chi-Chen; Tang, Jen-Yang; Chang, Hsueh-Wei

    2013-06-03

    For their various bioactivities, biomaterials derived from marine algae are important ingredients in many products, such as cosmetics and drugs for treating cancer and other diseases. This mini-review comprehensively compares the bioactivities and biological functions of biomaterials from red, green, brown, and blue-green algae. The anti-oxidative effects and bioactivities of several different crude extracts of algae have been evaluated both in vitro and in vivo. Natural products derived from marine algae protect cells by modulating the effects of oxidative stress. Because oxidative stress plays important roles in inflammatory reactions and in carcinogenesis, marine algal natural products have potential for use in anti-cancer and anti-inflammatory drugs.

  20. Model independent calculation of B(anti B0→D(*)+τ- anti ν)/B(anti B0→D(*)+e- anti ν)

    International Nuclear Information System (INIS)

    Hwang, D.S.

    2000-01-01

    Using the formulas for the dΓ/dq 2 distribution with non-zero lepton mass and experimentally determined form factors, we calculate the dΓ(D (*)+ l - anti ν)/dq 2 spectra and branching fractions for l=e,μ and τ. We obtain the results B(anti B 0 →D + τ - anti ν)/B(anti B 0 →D + e - anti ν)=0.278 +0.049 -0.035 and B(anti B 0 →D *+ τ - anti ν)/B(anti B 0 →D *+ e - anti ν)=0.256 +0.014 -0.013 . Since we used the experimentally measured form factors, these results are independent of theoretical models of the form factors. (orig.)

  1. Equilibrated anti-Monge Matrices

    Czech Academy of Sciences Publication Activity Database

    Fiedler, Miroslav

    2001-01-01

    Roč. 335, - (2001), s. 151-156 ISSN 0024-3795 R&D Projects: GA ČR GA201/98/0222 Institutional research plan: AV0Z1030915 Keywords : Monge matrix * anti-Monge matrix Subject RIV: BA - General Mathematics Impact factor: 0.423, year: 2001<