WorldWideScience

Sample records for antibodies final performance

  1. Final Performance Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Houldin, Joseph [Delaware Valley Industrial Resource Center, Philadelphia, PA (United States); Saboor, Veronica [Delaware Valley Industrial Resource Center, Philadelphia, PA (United States)

    2016-03-30

    about assessing a company’s technical assets, broadening our view of the business to go beyond what they make or what NAICS code they have…to better understand their capacity, capability, and expertise, and to learn more about THEIR customers. Knowing more about the markets they serve can often provide insight into their level of technical knowledge and sophistication. Finally, in the spirit of realizing the intent of the Accelerator we strove to align and integrate the work and activities supported by the five funding agencies to leverage each effort. To that end, we include in the Integrated Work Plan a graphic that illustrates that integration. What follows is our summary report of the project, aggregated from prior reports.

  2. Final Performance Report

    Energy Technology Data Exchange (ETDEWEB)

    Hsieh, S. T. [Tulane Univ., New Orleans, LA (United States)

    2013-08-31

    U.S./China Energy and Environmental Technology Center (EETC), Payson Center for International Development, Law School of Tulane University was officially established in 1997 with initial funds from private sector, US Environmental Protection Agency and the US Department of Energy (DOE.) Lately, DOE has provided EETC funds for operations with cost share from the Ministry of Science and Technology, China. EETC was created to facilitate the development of friendly, broad-based U.S./China relations. Tulane University signed the Memorandum of Understanding (MOU) with the Chinese People’s Institute of Foreign Affairs (1995) to promote the formation of Chinese partners for EETC. EETC’s original goal is to enhance the competitiveness of US clean fossil energy technology in China so that, as her economy expands, local and global environment are well protected. Specifically, through the demonstration and broadly deployment of US developed clean coal technology for power generation, transmission, and emission reductions in China. EETC is also focused on US industry partnerships for local economic development. One of the main the objectives of the EETC is to promote the efficient, responsible production and utilization of energy with a focus on clean fossil energy, promote US clean energy and environmental technologies, and encourage environmental performance while improving the quality of life in China. Another objective is to assist China with environmental and energy policy development and provide supports for China’s development with expertise (best practices) from US industry.

  3. Power performance assessment. Final report

    International Nuclear Information System (INIS)

    Frandsen, S.

    1998-12-01

    In the increasingly commercialised wind power marketplace, the lack of precise assessment methods for the output of an investment is becoming a barrier for wider penetration of wind power. Thus, addressing this problem, the overall objectives of the project are to reduce the financial risk in investment in wind power projects by significantly improving the power performance assessment methods. Ultimately, if this objective is successfully met, the project may also result in improved tuning of the individual wind turbines and in optimisation methods for wind farm operation. The immediate, measurable objectives of the project are: To prepare a review of existing contractual aspects of power performance verification procedures of wind farms; to provide information on production sensitivity to specific terrain characteristics and wind turbine parameters by analyses of a larger number of wind farm power performance data available to the proposers; to improve the understanding of the physical parameters connected to power performance in complex environment by comparing real-life wind farm power performance data with 3D computational flow models and 3D-turbulence wind turbine models; to develop the statistical framework including uncertainty analysis for power performance assessment in complex environments; and to propose one or more procedures for power performance evaluation of wind power plants in complex environments to be applied in contractual agreements between purchasers and manufacturers on production warranties. Although the focus in this project is on power performance assessment the possible results will also be of benefit to energy yield forecasting, since the two tasks are strongly related. (au) JOULE III. 66 refs.; In Co-operation Renewable Energy System Ltd. (GB); Centre for Renewable Energy (GR); Aeronautic Research Centre (SE); National Engineering Lab. (GB); Public Power Cooperation (GR)

  4. High performance MEAs. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-07-15

    The aim of the present project is through modeling, material and process development to obtain significantly better MEA performance and to attain the technology necessary to fabricate stable catalyst materials thereby providing a viable alternative to current industry standard. This project primarily focused on the development and characterization of novel catalyst materials for the use in high temperature (HT) and low temperature (LT) proton-exchange membrane fuel cells (PEMFC). New catalysts are needed in order to improve fuel cell performance and reduce the cost of fuel cell systems. Additional tasks were the development of new, durable sealing materials to be used in PEMFC as well as the computational modeling of heat and mass transfer processes, predominantly in LT PEMFC, in order to improve fundamental understanding of the multi-phase flow issues and liquid water management in fuel cells. An improved fundamental understanding of these processes will lead to improved fuel cell performance and hence will also result in a reduced catalyst loading to achieve the same performance. The consortium have obtained significant research results and progress for new catalyst materials and substrates with promising enhanced performance and fabrication of the materials using novel methods. However, the new materials and synthesis methods explored are still in the early research and development phase. The project has contributed to improved MEA performance using less precious metal and has been demonstrated for both LT-PEM, DMFC and HT-PEM applications. New novel approach and progress of the modelling activities has been extremely satisfactory with numerous conference and journal publications along with two potential inventions concerning the catalyst layer. (LN)

  5. High performance liquid chromatography in studies of radiolabeled antibodies

    International Nuclear Information System (INIS)

    Hnatowich, D.J.

    1986-01-01

    High performance liquid chromatography (HPLC) as applied to the separation of antibodies displays the same advantages as in its other applications, namely good resolution accompanied by fast analysis. It is therefore not surprising that many HPLC columns designed for use with antibodies and other proteins are now available commercially. The properties of proteins which provide the separation are size, hydrophobicity, charge and affinity. The features of each are discussed. (author)

  6. Final Report: Performance Engineering Research Institute

    Energy Technology Data Exchange (ETDEWEB)

    Mellor-Crummey, John [Rice Univ., Houston, TX (United States)

    2014-10-27

    This document is a final report about the work performed for cooperative agreement DE-FC02-06ER25764, the Rice University effort of Performance Engineering Research Institute (PERI). PERI was an Enabling Technologies Institute of the Scientific Discovery through Advanced Computing (SciDAC-2) program supported by the Department of Energy's Office of Science Advanced Scientific Computing Research (ASCR) program. The PERI effort at Rice University focused on (1) research and development of tools for measurement and analysis of application program performance, and (2) engagement with SciDAC-2 application teams.

  7. Final Progress Report: SPECT Assay of Radiolabeled Monoclonal Antibodies

    International Nuclear Information System (INIS)

    Jaszczak, Ronald J.

    2004-01-01

    During the past project period, we proposed to collaborate closely with DOE's Thomas Jefferson National Accelerator Facility (Jefferson Lab or JLab) to design a compact, ultra-high-resolution, high-sensitivity gamma camera for quantifying brain-tumor distributions of I-131. We also proposed to continue our on-going research in developing and evaluating pinhole collimation for quantitative ultra-high-resolution imaging of I-131-labeled MAbs. We have made excellent progress in accomplishing much of the research related to pinhole collimation. Many of the most significant results have been presented in peer-reviewed journal articles and conference proceedings. We have also made good progress in collaborating with JLab's Detector Group in developing a compact, ultra-high-resolution, gamma camera. A prototype I-131 imager was delivered to Duke on May 28, 2003. Our research results are summarized in the following sections. A. JLAB-DUKE DEDICATED BRAIN-TUMOR IMAGING SYSTEM A.1. Determination of Optimal Collimator Design During the current project period a prototype I-131 dedicated brain imager has been designed and built. Computer simulations and analysis of alternate designs were performed at Duke to determine an optimal collimator design. Collimator response was characterized by spatial resolution and sensitivity. Both geometric (non-penetrative) and penetrative sensitivities were considered in selecting an optimal collimator design. Based on these simulation results, two collimator designs were selected and built by external vendors. Initial imaging results were obtained using these collimators. B. INITIAL DEVELOPMENT OF SPECT RECONSTRUCTION SOFTWARE FOR JLAB-DUKE CAMERA B.1. Modeling Thick Septa and Collimator Holes: Geometrical-Phantom Study A geometrical phantom was designed to illuminate spatial resolution effects. The phantom includes a uniformly attenuating medium that consists of all voxels within an elliptical cylinder that is centered on the axis of rotation

  8. 10 CFR 603.890 - Final performance report.

    Science.gov (United States)

    2010-01-01

    ... to Other Administrative Matters Financial and Programmatic Reporting § 603.890 Final performance report. A TIA must require a final performance report that addresses all major accomplishments under the... 10 Energy 4 2010-01-01 2010-01-01 false Final performance report. 603.890 Section 603.890 Energy...

  9. Positron tomographic imaging of tumors using monoclonal antibodies. Final progress report, April 15, 1989--October 31, 1995

    Energy Technology Data Exchange (ETDEWEB)

    Zalutsky, M.R.

    1997-02-01

    The overall objective of this research is to develop methods for utilizing positron emission tomography (PET) to increase the clinical potential of radiolabeled monoclonal antibodies (MAbs). Enhancement of MAb tumor localization by hyperthermia also was proposed. Studies were to have been performed with both {sup 18}F and {sup 124}I; however, the lack of its availability (until quite recently) prevented experiments with {sup 124}I. Instead, two additional lines of inquiry were initiated in which they utilized aspects of the radiofluorination chemistries originally developed for MAbs for labeling chemotactic peptides and meta-iodobenzylguanidine (MIBG) analogues with {sup 18}F. This final report summarizes the original specific aims and the main research accomplishments in studies of mouse, dog and human models.

  10. Positron tomographic imaging of tumors using monoclonal antibodies. Final progress report, April 15, 1989--October 31, 1995

    International Nuclear Information System (INIS)

    Zalutsky, M.R.

    1997-02-01

    The overall objective of this research is to develop methods for utilizing positron emission tomography (PET) to increase the clinical potential of radiolabeled monoclonal antibodies (MAbs). Enhancement of MAb tumor localization by hyperthermia also was proposed. Studies were to have been performed with both 18 F and 124 I; however, the lack of its availability (until quite recently) prevented experiments with 124 I. Instead, two additional lines of inquiry were initiated in which they utilized aspects of the radiofluorination chemistries originally developed for MAbs for labeling chemotactic peptides and meta-iodobenzylguanidine (MIBG) analogues with 18 F. This final report summarizes the original specific aims and the main research accomplishments in studies of mouse, dog and human models

  11. Performance life of HMA mixes : final report.

    Science.gov (United States)

    2016-01-01

    A number of hot mix asphalt (HMA) types, such as permeable friction course (PFC), stone mastic asphalts : (SMA), performance design mixes and conventional dense graded mixes are currently used to construct or overlay : roads. One of the important inp...

  12. Measuring performance of parallel computers. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, F.

    1994-07-01

    Performance Measurement - the authors have developed a taxonomy of parallel algorithms based on data motion and example applications have been coded for each class of the taxonomy. Computational benchmark kernels have been extracted for several applications, and detailed measurements have been performed. Algorithms for Massively Parallel SIMD machines - measurement results and computational experiences indicate that top performance will be achieved by `iteration` type algorithms running on massively parallel SIMD machines. Reformulation as iteration may entail unorthodox approaches based on probabilistic methods. The authors have developed such methods for some applications. Here they discuss their approach to performance measurement, describe the taxonomy and measurements which have been made, and report on some general conclusions which can be drawn from the results of the measurements.

  13. Nuclear fuel performance evaluation. Final report

    International Nuclear Information System (INIS)

    Boerresen, S.; Pomeroy, D.L.; Rolstad, E.; Sauar, T.O.

    1977-06-01

    An evaluation has been made of the ability of Scandpower's empirical fuel performance model POSHO (''Power Shock'') to predict the probability of fuel pin failures resulting from pellet-clad interaction in commercial nuclear power plants. POSHO provides an analytical method to calculate the failure probabilities associated with power level maneuvers for different fuel assembly designs. Application of the method provides a basis for risk-benefit decisions concerning operational procedures, fuel designs and fuel management strategies. One boiling water reactor (BWR) and one pressurized water reactor (PWR) were selected for study to compare model predictions with actual failures, as determined from post irradiation examination of the fuel and activity release data. The fuel duty cycles were reconstructed from operating records and nodal power histories were created by using Scandpower's FMS computer programs. Nodal power histories, coupled with the relative pin power distribution in each node, were processed by the fuel failure prediction model, which tracks the interaction power level for each pin group in each node and calculates the power shocks and the probability for pellet-clad interaction cracks. The results of these calculations are processed statistically to give the expected number of cracks, the number of failed fuel pins in each assembly and the total number of failed assemblies in the core. Fuel performance in the BWR, Quad Cities Unit Two, was calculated by the model in approximate agreement with the observed performance. Fuel performance in the PWR, Maine Yankee, was calculated in approximate agreement for two of the three fuel designs. The high failure rate in the third design, Type B fuel, was not calculated by the POSHO pellet-clad interaction model

  14. Performance of Personal Workspace Controls Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Rubinstein, Francis; Kiliccote, Sila; Loffeld, John; Pettler,Pete; Snook, Joel

    2004-12-01

    One of the key deliverables for the DOE-funded controls research at LBNL for FY04 was the development of a prototype Personal Workspace Control system. The successful development of this system is a critical milestone for the LBNL Lighting Controls Research effort because this system demonstrates how IBECS can add value to today's Task Ambient lighting systems. LBNL has argued that by providing both the occupant and the facilities manager with the ability to precisely control the operation of overhead lighting and all task lighting in a coordinated manner, that task ambient lighting can optimize energy performance and occupant comfort simultaneously [Reference Task Ambient Foundation Document]. The Personal Workspace Control system is the application of IBECS to this important lighting problem. This report discusses the development of the Personal Workspace Control to date including descriptions of the different fixture types that have been converted to IBECS operation and a detailed description of the operation of PWC Scene Controller, which provides the end user with precise control of his task ambient lighting system. The objective, from the Annual Plan, is to demonstrate improvements in efficiency, lighting quality and occupant comfort realized using Personal Workspace Controls (PWC) designed to optimize the delivery of lighting to the individual's workstation regardless of which task-ambient lighting solution is chosen. The PWC will be capable of controlling floor-mounted, desk lamps, furniture-mounted and overhead lighting fixtures from a personal computer and handheld remote. The PWC will use an environmental sensor to automatically monitor illuminance, temperature and occupancy and to appropriately modulate ambient lighting according to daylight availability and to switch off task lighting according to local occupancy. [Adding occupancy control to the system would blunt the historical criticism of occupant-controlled lighting - the tendency of the

  15. Amended Final Report - Antibodies to Radionuclides. Engineering by Surface Display for Immunosensors

    Energy Technology Data Exchange (ETDEWEB)

    Blake, Diane A. [Tulane Univ., New Orleans, LA (United States)

    2013-06-14

    The relatively new techniques of antibody display, which permit molecular engineering of antibody structure and function, have the potential to revolutionize the way scientists generate binding proteins for specific applications. However, the skills required to efficiently use antibody display techniques have proven difficult for other laboratories to acquire without hands-on training and exchange of laboratory personnel. This research project is designed bring important expertise in antibody display to the State of Louisiana while pursuing a project with direct relevance to the DOE’s EM program.

  16. Factors Influencing Student Nurses' Performance in the Final ...

    African Journals Online (AJOL)

    The purpose of this study was to identify leads to factors influencing student nurses' performance in the final year practical examination at Gweru School of Nursing. A descriptive survey was used to collect data from a census of 16 assessors and cluster sample of 35 student nurses. A questionnaire designed for the research ...

  17. 10 CFR 603.900 - Receipt of final performance report.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Receipt of final performance report. 603.900 Section 603.900 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS TECHNOLOGY INVESTMENT AGREEMENTS Award Terms Related to Other Administrative Matters Financial and Programmatic Reporting § 603.900...

  18. Sleep and Final Exam Performance in Introductory Physics

    Science.gov (United States)

    Coletta, Vincent; Wikholm, Colin; Pascoe, Daniel

    2018-03-01

    Most physics instructors believe that adequate sleep is important in order for students to perform well on problem solving, and many instructors advise students to get plenty of sleep the night before an exam. After years of giving such advice to students at Loyola Marymount University (LMU), one of us decided to find out how many hours students actually do sleep the night before an exam, and how that would relate to their performance. The effect of inadequate sleep on exam performance was explored in a second-semester introductory physics course. At the end of the final exam, students reported the number of hours they slept the night before. Sleep deprivation corresponded to lower final exam scores. The main purpose of this study is to provide evidence that instructors can provide to their students to convince them that their time is better spent sleeping rather than studying all night before an exam.

  19. Impact of immunization technology and assay application on antibody performance--a systematic comparative evaluation.

    Directory of Open Access Journals (Sweden)

    Michael C Brown

    Full Text Available Antibodies are quintessential affinity reagents for the investigation and determination of a protein's expression patterns, localization, quantitation, modifications, purification, and functional understanding. Antibodies are typically used in techniques such as Western blot, immunohistochemistry (IHC, and enzyme-linked immunosorbent assays (ELISA, among others. The methods employed to generate antibodies can have a profound impact on their success in any of these applications. We raised antibodies against 10 serum proteins using 3 immunization methods: peptide antigens (3 per protein, DNA prime/protein fragment-boost ("DNA immunization"; 3 per protein, and full length protein. Antibodies thus generated were systematically evaluated using several different assay technologies (ELISA, IHC, and Western blot. Antibodies raised against peptides worked predominantly in applications where the target protein was denatured (57% success in Western blot, 66% success in immunohistochemistry, although 37% of the antibodies thus generated did not work in any of these applications. In contrast, antibodies produced by DNA immunization performed well against both denatured and native targets with a high level of success: 93% success in Western blots, 100% success in immunohistochemistry, and 79% success in ELISA. Importantly, success in one assay method was not predictive of success in another. Immunization with full length protein consistently yielded the best results; however, this method is not typically available for new targets, due to the difficulty of generating full length protein. We conclude that DNA immunization strategies which are not encumbered by the limitations of efficacy (peptides or requirements for full length proteins can be quite successful, particularly when multiple constructs for each protein are used.

  20. Manufacture and Performance of the LHC Main Dipole Final Prototypes

    CERN Document Server

    Modena, M; Bajko, M; Bottura, L; Buzio, M; Fessia, P; Pagano, O; Perini, D; Savary, F; Scandale, Walter; Siemko, A; Spigo, G; Todesco, Ezio; Vanenkov, I; Vlogaert, J; Wyss, C

    2000-01-01

    This paper reports about the program of six LHC main dipole final prototypes. This program, launched in summer 1998, relies on industrially manufactured collared coils and cold masses assembled at the CERN Magnet Assembly Facility. The magnet design for series manufacture features a "6-block" coil and austenitic steel collars, following design, stability and robustness studies. Results of mechanical and magnetic measurements are given and discussed, as well as the performances of the prototypes measured so far.

  1. Effect of formulation on the stability and aerosol performance of a nebulized antibody.

    Science.gov (United States)

    Respaud, Renaud; Marchand, Denis; Parent, Christelle; Pelat, Thibaut; Thullier, Philippe; Tournamille, Jean-François; Viaud-Massuard, Marie-Claude; Diot, Patrice; Si-Tahar, Mustapha; Vecellio, Laurent; Heuzé-Vourc'h, Nathalie

    2014-01-01

    Most monoclonal antibodies (mAbs) are administered to patients intravenously to ensure high bioavailability as rapidly as possible. The airways, however, are an attractive delivery route for mAbs for the treatment of lung diseases, making it possible to increase their concentration in the target organ while limiting their systemic passage. Several challenges must be overcome for translation into clinical practice. For example, the drug and device must be paired for the efficient and reliable deposition of a pharmacologically active and safe mAb in the lung region of interest. Mesh nebulizers appear to be the most effective aerosol-producing devices for delivering large amounts of biopharmaceutical while limiting protein instability during nebulization. We used metrological and analytic methods to analyze the effect of both antibody concentration and surfactant addition on aerosol performance and antibody integrity. These two factors had a limited effect on aerosol performance, but affected antibody aggregation. The addition of surfactants to antibody formulations at concentrations appropriate for lung administration markedly reduced the formation of medium or large aggregates, as shown by dynamic light scattering and fluorescence microscopy. Aggregation was also dependent on the type of mesh nebulizer, highlighting the need to optimize drug and device together.

  2. Novel antibody conjugates for enhanced tumor uptake. Final report, September 1, 1992--August 31, 1997

    International Nuclear Information System (INIS)

    Thakur, M.

    1997-01-01

    Progress in three areas of research is summarized. These are as follows: Labeling Monoclonal antibodies (MAbs) with Tc-99m and Re-186; human melanoma tumors and specific MAbs; evaluation of biological response modifiers (BRM). The techniques of labeling MAbs (IgM, IgG, F(ab') 2 or F(ab')) with Tc-99m was developed in the author's laboratory in 1989 and that with Re-186 in 1992. The techniques are in daily use in the laboratory since then and are adapted to a convenient kit formulation. The metal ions are bound at MAb sulfhydryls generated by a controlled reduction of a pair of disulfide groups. At least two types of MAbs labeled with Tc-99m by this method have been administered into patients and excellent diagnostic results have been obtained. Over the past two and a half years the author has been successfully growing human melanoma tumors in athymic Balb/c nude mice. The cell LINE, WM-9, was obtained from Dr. D Herlyn's laboratory at Wistar Institute in Philadelphia. Sufficient quantities of antihuman melanoma specific antibodies ME 31.3 (Wistar, IgG-1) and MEM-136 (Hybritech, IgG-2A) and their F(ab') 2 fragments are also available in the laboratory. The use of BRM is a rapidly evolving field. Over the past four years, the author has evaluated a number of BRMs in a quest for agents that may augment MAb tumor uptake. These included interferon-α; a pokeweed mitogen and Ukrain, an alkaloid separated from a plant Chelideonium Majis. In these preliminary studies, normal Balb/c mice were used and the BRMs were given i.p. one hour prior to the i.v. administration of tumor necrosis factor or an MAb (TNT-F(ab') 2 ) labeled with Tc-99m which served as an imaging agent. Animals were sacrificed at 1.5 hr or 4 hrs post-injection. Highlights of the work are given here in a table

  3. French Modular Impoundment: Final Cost and Performance Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Drown, Peter [French Development Enterprises, LLC, North Billerica, MA (United States); French, Bill [French Development Enterprises, LLC, North Billerica, MA (United States)

    2017-05-17

    This report comprises the Final Cost and Performance Report for the Department of Energy Award # EE0007244, the French Modular Impoundment (aka the “French Dam”.) The French Dam is a system of applying precast modular construction to water control structures. The “French Dam” is a term used to cover the construction means/methods used to construct or rehabilitate dams, diversion structures, powerhouses, and other hydraulic structures which impound water and are covered under FDE’s existing IP (Patents # US8414223B2; US9103084B2.)

  4. A simple vector system to improve performance and utilisation of recombinant antibodies

    Directory of Open Access Journals (Sweden)

    Vincent Karen J

    2006-12-01

    Full Text Available Abstract Background Isolation of recombinant antibody fragments from antibody libraries is well established using technologies such as phage display. Phage display vectors are ideal for efficient display of antibody fragments on the surface of bacteriophage particles. However, they are often inefficient for expression of soluble antibody fragments, and sub-cloning of selected antibody populations into dedicated soluble antibody fragment expression vectors can enhance expression. Results We have developed a simple vector system for expression, dimerisation and detection of recombinant antibody fragments in the form of single chain Fvs (scFvs. Expression is driven by the T7 RNA polymerase promoter in conjunction with the inducible lysogen strain BL21 (DE3. The system is compatible with a simple auto-induction culture system for scFv production. As an alternative to periplasmic expression, expression directly in the cytoplasm of a mutant strain with a more oxidising cytoplasmic environment (Origami 2™ (DE3 was investigated and found to be inferior to periplasmic expression in BL21 (DE3 cells. The effect on yield and binding activity of fusing scFvs to the N terminus of maltose binding protein (a solubility enhancing partner, bacterial alkaline phosphatase (a naturally dimeric enzymatic reporter molecule, or the addition of a free C-terminal cysteine was determined. Fusion of scFvs to the N-terminus of maltose binding protein increased scFv yield but binding activity of the scFv was compromised. In contrast, fusion to the N-terminus of bacterial alkaline phosphatase led to an improved performance. Alkaline phosphatase provides a convenient tag allowing direct enzymatic detection of scFv fusions within crude extracts without the need for secondary reagents. Alkaline phosphatase also drives dimerisation of the scFv leading to an improvement in performance compared to monovalent constructs. This is illustrated by ELISA, western blot and

  5. Development of High-Performance Cast Crankshafts. Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Bauer, Mark E [General Motors, Detroit, MI (United States)

    2017-03-31

    The objective of this project was to develop technologies that would enable the production of cast crankshafts that can replace high performance forged steel crankshafts. To achieve this, the Ultimate Tensile Strength (UTS) of the new material needs to be 850 MPa with a desired minimum Yield Strength (YS; 0.2% offset) of 615 MPa and at least 10% elongation. Perhaps more challenging, the cast material needs to be able to achieve sufficient local fatigue properties to satisfy the durability requirements in today’s high performance gasoline and diesel engine applications. The project team focused on the development of cast steel alloys for application in crankshafts to take advantage of the higher stiffness over other potential material choices. The material and process developed should be able to produce high-performance crankshafts at no more than 110% of the cost of current production cast units, perhaps the most difficult objective to achieve. To minimize costs, the primary alloy design strategy was to design compositions that can achieve the required properties with minimal alloying and post-casting heat treatments. An Integrated Computational Materials Engineering (ICME) based approach was utilized, rather than relying only on traditional trial-and-error methods, which has been proven to accelerate alloy development time. Prototype melt chemistries designed using ICME were cast as test specimens and characterized iteratively to develop an alloy design within a stage-gate process. Standard characterization and material testing was done to validate the alloy performance against design targets and provide feedback to material design and manufacturing process models. Finally, the project called for Caterpillar and General Motors (GM) to develop optimized crankshaft designs using the final material and manufacturing processing path developed. A multi-disciplinary effort was to integrate finite element analyses by engine designers and geometry-specific casting

  6. Effects of Dietary Supplemental Vitamins and Periods of Administration on Growth Performance and Antibody Titre of Broiler Chickens Vaccinated against Newcastle Disease

    Directory of Open Access Journals (Sweden)

    Odutayo, O. J.

    2016-12-01

    Full Text Available This study investigated the effects of supplemental vitamins and varying administration periods on growth performance and antibody titre of broiler chickens vaccinated against Newcastle Disease (ND. A total of 300 unvaccinated against ND Arbor Acre day-old chicks were used for the study for 8 wk. Birds were brooded together on day 1 of age, and 30 chicks were selected randomly for evaluating the maternally derived antibody titre against ND. At 2 days of age, the remaining 270 chicks were divided based on weight equalization into 9 treatment groups and replicated thrice. The 9 treatments consisted of a factorial arrangement of 4 supplemental vitamins (A, C, E and combination of A, C, E and 2 periods of administration (3 days pre- and post-ND vaccinations with a control. The birds were managed intensively throughout the experimental period, ND vaccines were administered on the 5th (i/o and 24th (Lasota day of age, respectively. Supplemental combined vitamins A, C and E at 0.15, 16.67 and 3.03 mg/kg, respectively, resulted in higher (P < 0.05 final body weight of 1785.00 g/bird and better feed conversion ratio (FCR of 2.89. Also, birds fed vitamin A supplemented diet 3 d pre-i/o vaccine had higher (p<0.05 serum antibody titre (75.20 against ND while higher (p<0.05 serum antibody titre (741.33 was also obtained in birds fed diet supplemented with vitamin E 3 d post-Lasota vaccination. Conclusively, broiler chickens diets can be supplemented with combined vitamins A, C, and E for better growth performance measured as final body weight and FCR, in addition, vitamins A (0.45mg/kg and E (9.1mg/kg dietary supplementation at 3 d pre-i/o and 3 d post-Lasota vaccines, respectively, can be adopted for improved antibody production.

  7. Performance Evaluation of CLIA for Treponema Pallidum Specific Antibodies Detection in Comparison with ELISA.

    Science.gov (United States)

    Li, Lixin; Cai, Bei; Tao, Chuanmin; Wang, Lanlan

    2016-05-01

    In this study we aimed to evaluate the performance effects of chemiluminescence assay (CLIA) for Treponema pallidum specific antibodies detection, and to compare T. pallidum specific antibodies detection accuracy between CLIA and ELISA with TPPA (T. pallidum particle agglutination assay) as a confirmatory test. A total of 865 samples from suspected syphilis patients and preoperative patients were included, in which T. pallidum specific antibodies were simultaneously detected by CLIA and ELISA. Among them, 457 samples were determined by TPPA. All coefficients of variation (CVs) of ELISA in high-, median-, and low-level samples were more than 5% and the maximum CV was 54.39% in the low-level sample. CVs of CLIA in different-level samples were all below 5%. Among the three assays the Spearman correlation and Kappa coefficients were 0.771 (P ≤ 0.001) and 0.854 (P ≤ 0.001, CLIA vs. ELISA), 0.806 (P ≤ 0.001) and 0.897 (P ≤ 0.001, ELISA vs. TPPA), 0.937 (P ≤ 0.001) and 0.967 (P ≤ 0.001, CLIA vs. TPPA), respectively. The area under the receiver operating characteristic curve (AUC) of CLIA was higher than that of ELISA (0.994 vs. 0.989) with TPPA as the confirmatory test. In 18 discrepant samples the consistency rate between CLIA and TPPA was elevated compared with that between ELISA and TPPA (72.22% vs. 27.78%, P = 0.008). In gray zone, the consistency rate of CLIA with TPPA was higher than that of ELISA with TPPA (90.91% vs. 41.67%, P = 0.027). Compared with ELISA, CLIA is more reliable, sensitive and accurate to detect serum T. pallidum specific antibodies. In the future it may be an alternative test with higher sensitivity to ELISA. © 2015 Wiley Periodicals, Inc.

  8. Prebiotic and antimicrobials on performance, carcass characteristics, and antibody production in broilers

    Directory of Open Access Journals (Sweden)

    Maíra Fomentini

    2016-06-01

    Full Text Available ABSTRACT: To evaluate the effect of supplementation with mannan oligosaccharides, avilamycin and halquinol, alone or in combination, on the performance, carcass characteristics and antibody production in broilers (1-49 days old, male broiler chicks (n=1440; Cobb 500; one day old were housed and distributed into a completely randomized design into six treatments (eight replicates; 30 animals per pen. To produce the experimental diets, three types of performance enhancer additives were used. Halquinol (HAL, avilamycin (AVI and mannan oligosaccharides (MOS were included (alone or in combination in the basal diet (instead of corn starch. Effects of diet were observed on results of animal performance in the period 1-21 and 1-42 days old. Broilers fed with a diet without growth promoter showed lower weight gain in relation to those fed with diets with antimicrobials, MOS or a combination of them. In the period 1-49 days old, feed conversion increased in broilers fed with rations without promoter. At the end of the experimental period no influence of diets was observed on the carcass yield and cuts, and titles of specific antibodies to avian infectious bronchitis. The use of MOS and/or antimicrobials (AVI or HAL, alone or in combination, improves feed conversion of broilers reared until 49 days of age.

  9. Pecan Street Grid Demonstration Program. Final technology performance report

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2015-02-10

    This document represents the final Regional Demonstration Project Technical Performance Report (TPR) for Pecan Street Inc.’s (Pecan Street) Smart Grid Demonstration Program, DE-OE-0000219. Pecan Street is a 501(c)(3) smart grid/clean energy research and development organization headquartered at The University of Texas at Austin (UT). Pecan Street worked in collaboration with Austin Energy, UT, Environmental Defense Fund (EDF), the City of Austin, the Austin Chamber of Commerce and selected consultants, contractors, and vendors to take a more detailed look at the energy load of residential and small commercial properties while the power industry is undergoing modernization. The Pecan Street Smart Grid Demonstration Program signed-up over 1,000 participants who are sharing their home or businesses’s electricity consumption data with the project via green button protocols, smart meters, and/or a home energy monitoring system (HEMS). Pecan Street completed the installation of HEMS in 750 homes and 25 commercial properties. The program provided incentives to increase the installed base of roof-top solar photovoltaic (PV) systems, plug-in electric vehicles with Level 2 charging, and smart appliances. Over 200 participants within a one square mile area took advantage of Austin Energy and Pecan Street’s joint PV incentive program and installed roof-top PV as part of this project. Of these homes, 69 purchased or leased an electric vehicle through Pecan Street’s PV rebate program and received a Level 2 charger from Pecan Street. Pecan Street studied the impacts of these technologies along with a variety of consumer behavior interventions, including pricing models, real-time feedback on energy use, incentive programs, and messaging, as well as the corresponding impacts on Austin Energy’s distribution assets.The primary demonstration site was the Mueller community in Austin, Texas. The Mueller development, located less than three miles from the Texas State Capitol

  10. Course Syllabi and Their Effects on Students' Final Grade Performance.

    Science.gov (United States)

    Serafin, Ana Gil

    This study examined the relationship between the changes introduced in a course syllabus for a course titled "Instructional Strategies" and the final grades obtained by freshman and sophomore students in three successive academic periods. A sample of 150 subjects was randomly selected from students enrolled in the course at the…

  11. The Indiana Deaf-Blind Services Project. Final Performance Report.

    Science.gov (United States)

    Goehl, Karen S.

    This final report describes activities and accomplishments of the Indiana Deaf-Blind Services Project, a 3-year federally funded project to enhance and further develop coordinated direct services to children and youth, birth through age 2 and ages 18 through 21. It also was designed to provide technical assistance to public and private agencies…

  12. Heat pumps for geothermal applications: availability and performance. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Reistad, G.M.; Means, P.

    1980-05-01

    A study of the performance and availability of water-source heat pumps was carried out. The primary purposes were to obtain the necessary basic information required for proper evaluation of the role of water-source heat pumps in geothermal energy utilization and/or to identify the research needed to provide this information. The Search of Relevant Literature considers the historical background, applications, achieved and projected performance evaluations and performance improvement techniques. The commercial water-source heat pump industry is considered in regard to both the present and projected availability and performance of units. Performance evaluations are made for units that use standard components but are redesigned for use in geothermal heating.

  13. Final design and performance of in situ testing in Grimsel

    International Nuclear Information System (INIS)

    Fuentes-Cantillana, J.L.; Garcia-SiNeriz, J.L.

    1998-01-01

    This report is focused on the design, engineering, and construction aspects of the in situ test carried out at the Grimsel underground laboratory in Switzerland. This reproduces the AGP-granite concept of ENRESA for HLW repositories in crystalline rock. Two heaters, similar in dimensions and weight to the canisters in the reference concept, have been placed in a horizontal drift with a 2.28-m diameter, a total test length of 17.4 m, and backfilled with a total of 115.7 † of highly-compacted bentonite blocks. The backfilled area has been closed with a concrete plug which is 2.7 m thick. More than 600 sensors have been installed in the test to monitor different parameters such as temperature, pressures, humidity, etc., within both the buffer material and the host rock. The installation was completed and commissioned in February 1997, and then the heating phase, which will last for at least 3 years, was started. During this period, the test will basically be operated in an automatic mode, controlled and monitored from Spain via modem. The report is the Final Report from AITEMIN for Phase 4 of the project and includes a description of the test configuration and layout; the design, engineering, and manufacturing aspects of the different test components and equipment; the emplacement operation; and the as built information regarding the final position of the main components and the sensors. (Author)

  14. Final Report - Certifying the Performance of Small Wind Turbines

    Energy Technology Data Exchange (ETDEWEB)

    Sherwood, Larry [Small Wind Certification Council, Clifton Park, NY (United States)

    2015-08-28

    The Small Wind Certification Council (SWCC) created a successful accredited certification program for small and medium wind turbines using the funding from this grant. SWCC certifies small turbines (200 square meters of swept area or less) to the American Wind Energy Association (AWEA) Small Wind Turbine Performance and Safety Standard (AWEA Standard 9.1 – 2009). SWCC also certifies medium wind turbines to the International Electrical Commission (IEC) Power Performance Standard (IEC 61400-12-1) and Acoustic Performance Standard (IEC 61400-11).

  15. Transportation asset management : organizational performance and risk review : final report.

    Science.gov (United States)

    2012-11-15

    The 2012 Federal reauthorization of surface transportation programs, Moving Ahead for Progress in the 21st : Century (MAP-21) formally introduced performance-based decision making for investments in surface : transportation programs. This report revi...

  16. Heavy point frog performance under passenger vehicles : final report.

    Science.gov (United States)

    2016-06-01

    Federal Railroad Administration contracted with the Transportation Technology Center, Inc., Pueblo, Colorado, to conduct an : investigation of passenger vehicle performance running through heavy point frog (HPF) up to speeds of 110 mph. A NUCARS : ...

  17. Safety performance evaluation of converging chevron pavement markings : final report.

    Science.gov (United States)

    2014-12-01

    The objectives of this study were (1) to perform a detailed safety analysis of converging chevron : pavement markings, quantifying the potential safety benefits and developing an understanding of the : incident types addressed by the treatment, and (...

  18. SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

    Energy Technology Data Exchange (ETDEWEB)

    Jaszczak, R.J.

    1995-12-01

    Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons.

  19. SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

    International Nuclear Information System (INIS)

    Jaszczak, R.J.

    1995-12-01

    Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons

  20. Influence of antibody immobilization strategies on the analytical performance of a magneto-elastic immunosensor for Staphylococcus aureus detection.

    Science.gov (United States)

    Menti, C; Beltrami, M; Pozza, M D; Martins, S T; Henriques, J A P; Santos, A D; Missell, F P; Roesch-Ely, M

    2017-07-01

    Magneto-elastic (ME) sensors have a great advantage in microbiology due to their ability to be queried wirelessly. Staphylococcus aureus is one of the most common bacteria widespread in the environment and a major human pathogen related to numerous illnesses. Immunosensors are affinity-based assays where the analyte is highly selective. The immobilization of antibodies (Ab) is an important step in the development of such devices. This study compared the effects of two antibody immobilization strategies on the analytical performance of a magneto-elastic immunosensor: (1) random antibody covalent immobilization (CysAb) and (2) specific-oriented antibody covalent immobilization (PrGAb). Immunosensors were exposed to solutions containing S. aureus at different concentrations (10 4 to 10 8 CFU/ml) and sensor resonant frequencies were measured. In order to confirm that the frequency shifts were mainly caused by the binding of S. aureus to the sensor's surface, scanning electron microscope (SEM) and indirect immunofluorescence (IIF) images were taken after bacteria exposure at 10 8 CFU/ml. Sensor surfaces were further monitored by non-contact topographic atomic force microscopy (AFM) images. In the covalent-oriented strategy, PrG was first bound covalently to the surface, which in turn, then binds the anti-S. aureus antibody in an oriented manner. Topographic AFM images showed different surface patterns between the two antibody immobilization strategies. Specific-oriented antibody covalent immobilization (PrGAb) strategy gave the highest anti-S. aureus antibody immobilization density. Therefore, the covalent-oriented strategy presented the best performance for S. aureus capture, detecting 10 4 CFU/ml. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. Performance of a multiplexed serological microarray for the detection of antibodies against central nervous system pathogens.

    Science.gov (United States)

    Jääskeläinen, Anne J; Viitala, Sari M; Kurkela, Satu; Hepojoki, Satu; Sillanpää, Heidi; Kallio-Kokko, Hannimari; Bergström, Tomas; Suni, Jukka; Närvänen, Ale; Vapalahti, Olli; Vaheri, Antti

    2014-05-01

    Central nervous system (CNS) infections have multiple potential causative agents for which simultaneous pathogen screening can provide a useful tool. This study evaluated a multiplexed microarray for the simultaneous detection of antibodies against CNS pathogens. The performance of selected microarray antigens for the detection of IgG antibodies against herpes simplex virus 1 and 2 (HSV-1 and HSV-2), varicella-zoster virus (VZV), adenovirus, Mycoplasma pneumoniae and Borrelia burgdorferi sensu lato, was evaluated using serum sample panels tested with reference assays used in a routine diagnostic laboratory. The microarray sensitivity for HSV-1, HSV-2, VZV, adenovirus and M. pneumonia ranged from 77% to 100%, and the specificity ranged from 74% to 97%. Very variable sensitivities and specificities were found for borrelial antigens of three different VlsE protein IR(6) peptide variants (IR6p1, IR6p2, IR6p4) and three recombinant decorin binding proteins A (DbpA; DbpAIa, DbpA91, DbpAG40). For single antigens, good specificity was shown for antigens of IR6p4 and DbpAIa (96%), while DbpA91, IR6p1 and IR6p2 were moderately specific (88-92%). The analytical sensitivity of the microarray was dependent on the borrelial IgG concentration of the specimen. The overall performance and technical features of the platform showed that the platform supports both recombinant proteins, whole viruses and peptides as antigens. This study showed diagnostic potential for all six CNS pathogens, including Borrelia burgdorferi sensu lato, using glutaraldehyde based microarray, and further highlighted the importance of careful antigen selection and the requirement for the use of multiple borrelial antigens in order to increase specificity without a major lack of sensitivity. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. Environmental-performance research priorities: Wood products. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-01-15

    This report describes a research plan to establish environmental, energy, and economic performance measures for renewable building materials, and to identify management and technology alternatives to improve environmental performance in a cost-effective manner. The research plan is designed to: (1) collect environmental and economic data on all life-cycle stages of the materials, (2) ensure that the data follows consistent definitions and collection procedures, and (3) develop analytical procedures for life-cycle analysis to address environmental performance questions. The research will be subdivided into a number of individual project modules. The five processing stages of wood used to organize the research plan are: (1) resource management and harvesting; (2) processing; (3) design and construction of structures; (4) use, maintenance, and disposal; and (5) waste recycling. Individual research module descriptions are provided in the report, as well as assessment techniques, research standards and protocol, and research management. 13 refs., 5 figs., 3 tabs.

  3. Sleep and Final Exam Performance in Introductory Physics

    Science.gov (United States)

    Coletta, Vincent; Wikholm, Colin; Pascoe, Daniel

    2018-01-01

    Most physics instructors believe that adequate sleep is important in order for students to perform well on problem solving, and many instructors advise students to get plenty of sleep the night before an exam. After years of giving such advice to students at Loyola Marymount University (LMU), one of us decided to find out how many hours students…

  4. Performance Measures for Federal Agency Websites: Final Report.

    Science.gov (United States)

    McClure, Charles R.; Sprehe, J. Timothy; Eschenfelder, Kristen

    The purpose of this study was to develop performance measures that will assist federal agencies to assess the quality and usefulness of their World Wide Web sites and to improve public information access service of those Web sites. The study was sponsored jointly by three federal agencies: the Defense Technical Information Center, the Energy…

  5. Mathematical Models of Elementary Mathematics Learning and Performance. Final Report.

    Science.gov (United States)

    Suppes, Patrick

    This project was concerned with the development of mathematical models of elementary mathematics learning and performance. Probabilistic finite automata and register machines with a finite number of registers were developed as models and extensively tested with data arising from the elementary-mathematics strand curriculum developed by the…

  6. High Performance Building Facade Solutions - PIER Final Project Report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eleanor; Selkowitz, Stephen

    2009-12-31

    Building facades directly influence heating and cooling loads and indirectly influence lighting loads when daylighting is considered, and are therefore a major determinant of annual energy use and peak electric demand. Facades also significantly influence occupant comfort and satisfaction, making the design optimization challenge more complex than many other building systems.This work focused on addressing significant near-term opportunities to reduce energy use in California commercial building stock by a) targeting voluntary, design-based opportunities derived from the use of better design guidelines and tools, and b) developing and deploying more efficient glazings, shading systems, daylighting systems, facade systems and integrated controls. This two-year project, supported by the California Energy Commission PIER program and the US Department of Energy, initiated a collaborative effort between The Lawrence Berkeley National Laboratory (LBNL) and major stakeholders in the facades industry to develop, evaluate, and accelerate market deployment of emerging, high-performance, integrated facade solutions. The LBNL Windows Testbed Facility acted as the primary catalyst and mediator on both sides of the building industry supply-user business transaction by a) aiding component suppliers to create and optimize cost effective, integrated systems that work, and b) demonstrating and verifying to the owner, designer, and specifier community that these integrated systems reliably deliver required energy performance. An industry consortium was initiated amongst approximately seventy disparate stakeholders, who unlike the HVAC or lighting industry, has no single representative, multi-disciplinary body or organized means of communicating and collaborating. The consortium provided guidance on the project and more importantly, began to mutually work out and agree on the goals, criteria, and pathways needed to attain the ambitious net zero energy goals defined by California and

  7. Influence choreographic readiness to gymnasts final assessment of performance skills

    Directory of Open Access Journals (Sweden)

    O.A. Omelichyk-Ziurkalova

    2014-10-01

    Full Text Available Purpose : to provide a quantitative assessment and expert choreographic preparedness gymnasts. Material : the study involved eight gymnasts competition finalists in the floor exercise - female members of the Ukrainian national team in gymnastics. Results : the quantitative indicators of acrobatic and dance elements to determine the baseline assessment. Defined methods complications composition on the floor exercise by reducing the number of acrobatic lines and diagonals and increase the number of gymnastic elements. The theoretical performance of the composite sequence is improved structure and increases the difficulty of the exercise. Conclusions : in the process of composition complications need to pay more attention to the technique of performing gymnastic elements. In improving exercise choreography element replace (in some cases acrobatic element. Based on the results is planned future direction of research in order to improve the training process in gymnastics.

  8. Coal-fired high performance power generating system. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-31

    As a result of the investigations carried out during Phase 1 of the Engineering Development of Coal-Fired High-Performance Power Generation Systems (Combustion 2000), the UTRC-led Combustion 2000 Team is recommending the development of an advanced high performance power generation system (HIPPS) whose high efficiency and minimal pollutant emissions will enable the US to use its abundant coal resources to satisfy current and future demand for electric power. The high efficiency of the power plant, which is the key to minimizing the environmental impact of coal, can only be achieved using a modern gas turbine system. Minimization of emissions can be achieved by combustor design, and advanced air pollution control devices. The commercial plant design described herein is a combined cycle using either a frame-type gas turbine or an intercooled aeroderivative with clean air as the working fluid. The air is heated by a coal-fired high temperature advanced furnace (HITAF). The best performance from the cycle is achieved by using a modern aeroderivative gas turbine, such as the intercooled FT4000. A simplified schematic is shown. In the UTRC HIPPS, the conversion efficiency for the heavy frame gas turbine version will be 47.4% (HHV) compared to the approximately 35% that is achieved in conventional coal-fired plants. This cycle is based on a gas turbine operating at turbine inlet temperatures approaching 2,500 F. Using an aeroderivative type gas turbine, efficiencies of over 49% could be realized in advanced cycle configuration (Humid Air Turbine, or HAT). Performance of these power plants is given in a table.

  9. Sacramento State Solar Decathlon 2015: Research Performance Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Mikael [California State Univ., Sacramento, CA (United States). Dept. of Construction Management

    2017-03-14

    Our primary objective is to design and build a 600-1000sf home that produces more energy than it consumes and to showcase this home at the 2015 Solar Decathlon in Irvine, CA. Further objectives are to educate consumers and home builders, alike (including K-12 students – the industry’s future consumers), inspire a shift towards the adoption of net-zero energy solutions in residential building, and to be a leader in the transformation of the California residential marketplace to a net-zero standard. Our specific mission statement for this project is as follows: Solar NEST strives to discover the future of sustainable, energy-efficient housing and deliver these innovations to home buyers at an affordable price. To make substantial improvements to conventional building methods with regard to aesthetics, performance, and affordability. Through our efforts, we aspire to bridge the gap between ‘what is’ and ‘what is possible’ by providing unique, elegant simplicity.

  10. Evolving cellular automata to perform computations. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    Crutchfield, J.P.; Mitchell, M.

    1998-04-01

    The overall goals of the project are to determine the usefulness of genetic algorithms (GAs) in designing spatially extended parallel systems to perform computational tasks and to develop theoretical frameworks both for understanding the computation in the systems evolved by the GA and for understanding the evolutionary process which successful systems are designed. In the original proposal the authors scheduled the first year of the project to be devoted to experimental grounding. During the first year they developed the simulation and graphics software necessary for doing experiments and analysis on one dimensional cellular automata (CAs), and they performed extensive experiments and analysis concerning two computational tasks--density classification and synchronization. Details of these experiments and results, and a list of resulting publications, were given in the 1994--1995 report. The authors scheduled the second year to be devoted to theoretical development. (A third year, to be funded by the National Science Foundation, will be devoted to applications.) Accordingly, most of the effort during the second year was spent on theory, both of GAs and of the CAs that they evolve. A central notion is that of the computational strategy of a CA, which they formalize in terms of domains, particles, and particle interactions. This formalization builds on the computational mechanics framework developed by Crutchfield and Hanson for understanding intrinsic computation in spatially extended dynamical systems. They have made significant progress in the following areas: (1) statistical dynamics of GAs; (2) formalizing particle based computation in cellular automata; and (3) computation in two-dimensional CAs.

  11. Intelligent Facades for High Performance Green Buildings. Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Dyson, Anna [Rensselaer Polytechnic Inst., Troy, NY (United States)

    2017-03-01

    Intelligent Facades for High Performance Green Buildings: Previous research and development of intelligent facades systems has been limited in their contribution towards national goals for achieving on-site net zero buildings, because this R&D has failed to couple the many qualitative requirements of building envelopes such as the provision of daylighting, access to exterior views, satisfying aesthetic and cultural characteristics, with the quantitative metrics of energy harvesting, storage and redistribution. To achieve energy self-sufficiency from on-site solar resources, building envelopes can and must address this gamut of concerns simultaneously. With this project, we have undertaken a high-performance building- integrated combined-heat and power concentrating photovoltaic system with high temperature thermal capture, storage and transport towards multiple applications (BICPV/T). The critical contribution we are offering with the Integrated Concentrating Solar Façade (ICSF) is conceived to improve daylighting quality for improved health of occupants and mitigate solar heat gain while maximally capturing and transferring on- site solar energy. The ICSF accomplishes this multi-functionality by intercepting only the direct-normal component of solar energy (which is responsible for elevated cooling loads) thereby transforming a previously problematic source of energy into a high- quality resource that can be applied to building demands such as heating, cooling, dehumidification, domestic hot water, and possible further augmentation of electrical generation through organic Rankine cycles. With the ICSF technology, our team is addressing the global challenge in transitioning commercial and residential building stock towards on-site clean energy self-sufficiency, by fully integrating innovative environmental control systems strategies within an intelligent and responsively dynamic building envelope. The advantage of being able to use the entire solar spectrum for

  12. A comparison of protein A chromatographic stationary phases: performance characteristics for monoclonal antibody purification.

    Science.gov (United States)

    Liu, Zhuo; Mostafa, Sigma S; Shukla, Abhinav A

    2015-01-01

    Protein A chromatography remains the dominant capture step used during the downstream purification of monoclonal antibodies (mAbs). With the recent expiry of the Repligen patent on recombinant Protein A, a variety of new Protein A resins have been introduced in the market. Given productivity limitations during downstream processing that have come into sharper focus with the recent increase in cell culture titers for mAbs, the selection of an appropriate Protein A resin has direct implications on the overall process economics of mAb production. The performance of seven different Protein A chromatographic resins was compared with respect to static binding capacity and dynamic binding capacity as a function of flow rate. This data was translated into a comparison of productivity (g mAb purified per unit resin volume per unit time) for the seven stationary phases. In addition, elution pH and host cell protein impurity levels after product capture on each of these resins were determined. The current article provides an effective methodology and dataset for the selection of the optimal Protein A chromatographic resin. © 2014 International Union of Biochemistry and Molecular Biology, Inc.

  13. Operations-oriented performance measures for freeway management systems : final report.

    Science.gov (United States)

    2008-12-01

    This report describes the second and final year activities of the project titled Using Operations-Oriented Performance Measures to Support Freeway Management Systems. Work activities included developing a prototype system architecture for testi...

  14. Effect of Repeated/Spaced Formative Assessments on Medical School Final Exam Performance

    Directory of Open Access Journals (Sweden)

    Edward K. Chang

    2017-06-01

    Discussion: Performance on weekly formative assessments was predictive of final exam scores. Struggling medical students will benefit from extra cumulative practice exams while students who are excelling do not need extra practice.

  15. Mastoidectomy performance assessment of virtual simulation training using final-product analysis

    DEFF Research Database (Denmark)

    Andersen, Steven A W; Cayé-Thomasen, Per; Sørensen, Mads S

    2015-01-01

    in virtual simulation and traditional dissection training. STUDY DESIGN: Prospective trial with blinding. METHODS: A total of 34 novice residents performed a mastoidectomy on the Visible Ear Simulator and on a cadaveric temporal bone. Two blinded senior otologists assessed the final-product performance using...... version of a validated final-product performance assessment tool can be used to assess mastoidectomy on virtual temporal bones. Performance assessment of virtual mastoidectomy could potentially save the use of cadaveric temporal bones for more advanced training when a basic level of competency......OBJECTIVES/HYPOTHESIS: The future development of integrated automatic assessment in temporal bone virtual surgical simulators calls for validation against currently established assessment tools. This study aimed to explore the relationship between mastoidectomy final-product performance assessment...

  16. Parameters concerning the preparation and performance of a magnetic microparticle antibody

    International Nuclear Information System (INIS)

    Rongsen, Shen; Ruiyun, Xing; Fengqi, Zhou; Xiuzhen, Liu; Dingquan, Wang

    1997-01-01

    We have described 'Magnetic Microparticle Antibodies and Their Application to RIAs' in a recently published paper. In this article operative parameters for the preparation of a magnetic second antibody (MSA-II) including results of purification of donkey anti-rabbit (D X R) serum by an (NH 4 ) 2 SO 4 precipitation method, rates of recovery of products in preparation of magnetic nucleus (MN, Fe 3 O 4 microparticle), in distillation of acrolein (AL) and in preparation of polyacrolein magnetic particle (PAMP), change in pH value of suspension irradiated before and after 60 Co γ-irradiation and volume of wet sediment in separation of magnetic particles by a magnetic separator, etc., as well as correlation of levels of quality control (QC) sera obtained with liquid-phase double antibody assay (LDA) and MSA-II assay during four years were supplementarily summarized. These operative parameters would be helpful to mastering the procedures for preparation and/or use of the magnetic particles. The better correlation of levels of QC sera for both the assays showed the reliability of the magnetic antibody. (author)

  17. Tumour auto-antibody screening: performance of protein microarrays using SEREX derived antigens

    International Nuclear Information System (INIS)

    Stempfer, René; Weinhäusel, Andreas; Syed, Parvez; Vierlinger, Klemens; Pichler, Rudolf; Meese, Eckart; Leidinger, Petra; Ludwig, Nicole; Kriegner, Albert; Nöhammer, Christa

    2010-01-01

    The simplicity and potential of minimal invasive testing using serum from patients make auto-antibody based biomarkers a very promising tool for use in diagnostics of cancer and auto-immune disease. Although several methods exist for elucidating candidate-protein markers, immobilizing these onto membranes and generating so called macroarrays is of limited use for marker validation. Especially when several hundred samples have to be analysed, microarrays could serve as a good alternative since processing macro membranes is cumbersome and reproducibility of results is moderate. Candidate markers identified by SEREX (serological identification of antigens by recombinant expression cloning) screenings of brain and lung tumour were used for macroarray and microarray production. For microarray production recombinant proteins were expressed in E. coli by autoinduction and purified His-tag (histidine-tagged) proteins were then used for the production of protein microarrays. Protein arrays were hybridized with the serum samples from brain and lung tumour patients. Methods for the generation of microarrays were successfully established when using antigens derived from membrane-based selection. Signal patterns obtained by microarrays analysis of brain and lung tumour patients' sera were highly reproducible (R = 0.92-0.96). This provides the technical foundation for diagnostic applications on the basis of auto-antibody patterns. In this limited test set, the assay provided high reproducibility and a broad dynamic range to classify all brain and lung samples correctly. Protein microarray is an efficient means for auto-antibody-based detection when using SEREX-derived clones expressing antigenic proteins. Protein microarrays are preferred to macroarrays due to the easier handling and the high reproducibility of auto-antibody testing. Especially when using only a few microliters of patient samples protein microarrays are ideally suited for validation of auto-antibody

  18. Anti-citrullinated protein antibodies in the diagnosis of rheumatoid arthritis (RA): diagnostic performance of automated anti-CCP-2 and anti-CCP-3 antibodies assays.

    Science.gov (United States)

    Vos, Ine; Van Mol, Christof; Trouw, Leendert A; Mahler, Michael; Bakker, Jaap A; Van Offel, Jan; De Clerck, Luc; Huizinga, Tom W

    2017-07-01

    This study compares the diagnostic performance of a second generation anti-cyclic citrullinated peptide antibody (CCP2) with a third generation anti-CCP antibodies assay (CCP3), as well as the combination of both tests. Serum samples of 127 patients were analyzed. IgG anti-CCP 2 and IgM rheumatoid factor were determined by EliA™ technique on a Phadia 250 instrument (Thermo Fisher Scientific), anti-CCP3 by the Quanta Flash™ anti-CCP3 IgG kit, BIO-FLASH Rapid Response Chemiluminscence Analyzer (INOVA Diagnostics). Diagnostic performance was compared using ROC-curves, sensitivity, specificity, likelihood ratios, and predictive values. Logistic regressions were used to investigate whether using both tests (anti-CCP2 and anti-CCP3) gives a better prediction of rheumatoid arthritis. At the manufacturer's cut-offs sensitivity and specificity were 79.4 and 61.0% for CCP3 and 80.9 and 69.5% for CCP2. No significant differences could be observed regarding the areas under the curve (AUC) of both ROC-curves. The optimal cut-off point for CCP2 was 10.5 U/ml (sensitivity of 75.0% and specificity of 80.0%) and 5.6 U/ml for CCP3 (sensitivity of 86.9% and specificity of 61.0%). Binary logistic regressions indicated that the likelihood of having rheumatoid arthritis (RA) is significantly higher when testing positive on both CCP2 and CCP3 compared to CCP2 or CCP3 alone. In our cohort, comparable performance was found between the two CCP assays. Positivity for both CCP2 and CCP3 resulted in the most specific identification of RA patients. In patients with joint complaints suspected of having RA and with a weakly positive CCP 2 (≥7 and ≤16 U/ml) CCP3 testing could be of additive value for diagnosing RA.

  19. Mastoidectomy performance assessment of virtual simulation training using final-product analysis.

    Science.gov (United States)

    Andersen, Steven A W; Cayé-Thomasen, Per; Sørensen, Mads S

    2015-02-01

    The future development of integrated automatic assessment in temporal bone virtual surgical simulators calls for validation against currently established assessment tools. This study aimed to explore the relationship between mastoidectomy final-product performance assessment in virtual simulation and traditional dissection training. Prospective trial with blinding. A total of 34 novice residents performed a mastoidectomy on the Visible Ear Simulator and on a cadaveric temporal bone. Two blinded senior otologists assessed the final-product performance using a modified Welling scale. The simulator gathered basic metrics on time, steps, and volumes in relation to the on-screen tutorial and collisions with vital structures. Substantial inter-rater reliability (kappa = 0.77) for virtual simulation and moderate inter-rater reliability (kappa = 0.59) for dissection final-product assessment was found. The simulation and dissection performance scores had significant correlation (P = .014). None of the basic simulator metrics correlated significantly with the final-product score except for number of steps completed in the simulator. A modified version of a validated final-product performance assessment tool can be used to assess mastoidectomy on virtual temporal bones. Performance assessment of virtual mastoidectomy could potentially save the use of cadaveric temporal bones for more advanced training when a basic level of competency in simulation has been achieved. NA. © 2014 The American Laryngological, Rhinological and Otological Society, Inc.

  20. Europium nanoparticle-based high performing immunoassay for the screening of treponemal antibodies.

    Directory of Open Access Journals (Sweden)

    Sheikh M Talha

    Full Text Available Treponema pallidum subspecies pallidum (Tp is the causative agent of syphilis which mainly spreads through sexual contact, blood transfusion and perinatal route. In order to curtail the spread of the infection and to clinically manage the disease, timely, accurate and reliable diagnosis is very important. We have developed an immunoassay for the detection of treponemal antibodies in human serum or plasma samples. In vivo biotinylated and non-biotinylated versions of the recombinant antigen were designed by the fusion of three Tp-specific antigens namely Tp15, Tp17 and Tp47. These fusion antigens were expressed in E. coli and purified using single-step metal affinity chromatography. Biotinylated fusion antigen immobilized on streptavidin coated plate was used to capture the treponemal antibodies and the non-biotinylated antigen coated on europium nanoparticles was used as tracer. Assays with two different incubation times of 10 min and 1 h were developed, and following the incubation the europium fluorescence was measured using time-resolved fluorometry. The developed time-resolved fluorometric (TRF immunoassays were evaluated with in-house and commercial serum/plasma sample panels. For well-established treponemal antibodies positive or negative samples, the sensitivity of TRF immunoassay with 10 min incubation time was 97.4%, and of TRF immunoassay with 1 h incubation time was 98.7%, and the specificities of both the TRF immunoassays were 99.2%. For the samples with discordant results with the reference assays, both the TRF immunoassays showed better specificity than the Enzygnost syphilis enzyme immunoassay as a screening test. The two different incubation times did not have any significant effect on the signal to cutoff (S/Co ratios obtained with the two immunoassays (p=0.06. Our results indicate that the developed immunoassay with a short incubation time of 10 min has the potential to be used in clinical laboratories and in blood

  1. In silico design and performance of peptide microarrays for breast cancer tumour-auto-antibody testing

    Directory of Open Access Journals (Sweden)

    Andreas Weinhäusel

    2012-06-01

    Full Text Available The simplicity and potential of minimally invasive testing using sera from patients makes auto-antibody based biomarkers a very promising tool for use in cancer diagnostics. Protein microarrays have been used for the identification of such auto-antibody signatures. Because high throughput protein expression and purification is laborious, synthetic peptides might be a good alternative for microarray generation and multiplexed analyses. In this study, we designed 1185 antigenic peptides, deduced from proteins expressed by 642 cDNA expression clones found to be sero-reactive in both breast tumour patients and controls. The sero-reactive proteins and the corresponding peptides were used for the production of protein and peptide microarrays. Serum samples from females with benign and malignant breast tumours and healthy control sera (n=16 per group were then analysed. Correct classification of the serum samples on peptide microarrays were 78% for discrimination of ‘malignant versus healthy controls’, 72% for ‘benign versus malignant’ and 94% for ‘benign versus controls’. On protein arrays, correct classification for these contrasts was 69%, 59% and 59%, respectively. The over-representation analysis of the classifiers derived from class prediction showed enrichment of genes associated with ribosomes, spliceosomes, endocytosis and the pentose phosphate pathway. Sequence analyses of the peptides with the highest sero-reactivity demonstrated enrichment of the zinc-finger domain. Peptides’ sero-reactivities were found negatively correlated with hydrophobicity and positively correlated with positive charge, high inter-residue protein contact energies and a secondary structure propensity bias. This study hints at the possibility of using in silico designed antigenic peptide microarrays as an alternative to protein microarrays for the improvement of tumour auto-antibody based diagnostics.

  2. Short communication: final year students' deficits in physical examination skills performance in Germany.

    Science.gov (United States)

    Krautter, Markus; Diefenbacher, Katja; Koehl-Hackert, Nadja; Buss, Beate; Nagelmann, Lars; Herzog, Wolfgang; Jünger, Jana; Nikendei, Christoph

    2015-01-01

    The physical examination of patients is an important diagnostic competence, but little is known about the examination skills of final-year medical students. To investigate physical examination skills of final-year medical students. In a cross-sectional study, 40 final-year students were asked to perform a detailed physical examination on standardized patients. Their performances were video-recorded and rated by independent video assessors. Video ratings showed a mean success rate of 40.1 % (SD 8.2). As regards accompanying doctor-patient communication, final-year students achieved a mean of no more than 36.7 % (SD 8.9) in the appropriate use of the corresponding communication items. Our study revealed severe deficits among final-year medical students in performing a detailed physical examination on a standardized patient. Thus, physical examination skills training should aim to improve these deficits while also paying attention to communicative aspects. Copyright © 2015. Published by Elsevier GmbH.

  3. Hospitality Industry Technology Training (HITT). Final Performance Report, April 1, 1989-December 31, 1990.

    Science.gov (United States)

    Mount Hood Community Coll., Gresham, OR.

    This final performance report includes a third-party evaluation and a replication guide. The first section describes a project to develop and implement an articulated curriculum for grades 8-14 to prepare young people for entry into hospitality/tourism-related occupations. It discusses the refinement of existing models, pilot test, curriculum…

  4. Contributing factors in the final performance of a common mode choke

    NARCIS (Netherlands)

    Roc'h, A.; Leferink, Frank Bernardus Johannes

    2015-01-01

    In order to avoid retro-designed common mode chokes in power system application, a predictive model is used to analyze the contributing factors in the final in-situ performance of the component. Designable parameters, together with environmental and installation aspects are analyzed. A hierarchic

  5. Development of monoclonal-antibody-based products for medical research and diagnostic imaging. Technical report, 28 January 1987-31 December 1988 (Final)

    International Nuclear Information System (INIS)

    Rhodes, B.A.; Pant, K.D.; Chauhan, N.; Buckelew, J.; Budd, P.

    1989-04-01

    Two major areas of application of monoclonal antibodies were examined: the development of products to support the 'Antibody Delivery System', a parent-specific and variable antibody formula drug system for use in imaging and treatment of cancer, and the development of an antibody-based radiopharmaceutical for imaging occult abscesses and other conditions involving high concentrations of white blood cells. In development of the Antibody Delivery System components, methods for characterization and purification of monoclonal antibodies were developed and validated; a dot immunoassay test, under the name RhoDot (TM) Immunoassay, was developed for matching antibodies to putative tumor specimen: a radioimmunoassay, under the name PhoChek (TM) Quality Control Test Kit for Radiolabeled Antibodies, was developed and commercialized for measuring the immunoreactive fraction of radiolabeled antibodies specific to colorecal cancer; and a patient-specific quality control test was developed. In development of the antibody-based radiopharmaceutical for imaging occult abscesses, a candidate antibody was identified and produced under U.S. Food and Drug Administration standards preparatory to human clinical trials

  6. Poor academic performance: A perspective of final year diagnostic radiography students

    International Nuclear Information System (INIS)

    Gqweta, Ntokozo

    2012-01-01

    Introduction: A study was conducted on final year diagnostic radiography students at a University of Technology in Durban. The aim of the study was to investigate the final year diagnostic radiography students' opinions and views on academic performance in order to inform teaching and learning methods. The objectives were: •To explore the students' opinions regarding poor performance. •To identify strategies to improve academic performance. Method: A qualitative, interpretive approach was used to explain and understand the students' lived experiences of their academic performances. A short open ended questionnaire was administered to a cohort of final diagnostic radiography students following feedback on a written assessment. Questionnaire responses were then manually captured and analyzed. Results: Five (5) themes were identified that could possibly be associated with poor academic performance. These themes were, poor preparation, lack of independent study, difficulty in understanding learning content and misinterpretation of assessment questions, inefficient studying techniques as well as perceived improvement strategies. Conclusion: Students identified their inadequate preparation and the lack of dedicated independent studying as the main reasons for poor performance. Students preferred to be taught in an assessment oriented manner. However their identified improvement strategies were aligned with the learner centred approach.

  7. Do peer-tutors perform better in examinations? An analysis of medical school final examination results.

    Science.gov (United States)

    Iwata, Kazuya; Furmedge, Daniel S; Sturrock, Alison; Gill, Deborah

    2014-07-01

    Peer-assisted learning (PAL) is recognised as an effective learning tool and its benefits are well documented in a range of educational settings. Learners find it enjoyable and their performances in assessments are comparable with those of students taught by faculty tutors. In addition, PAL tutors themselves report the development of improved clinical skills and confidence through tutoring. However, whether tutoring leads to actual improvement in performance has not been fully investigated. As high-achieving students are already en route to succeeding in final examinations, we wanted to examine whether participation in a peer-tutoring programme in itself leads to better final-year examination performance. We conducted a retrospective analysis of results on final-year written and clinical examinations at University College London Medical School during 2010-2012. Z-scores were calculated and the performances of PAL tutors and students who were not PAL tutors were compared using analysis of covariance (ancova). Year 4 examination results were used as indicators of previous academic attainment. Of the 1050 students who attempted the final examination, 172 were PAL tutors in the final year. Students who acted as PAL tutors outperformed students who did not in all examination components by 1-3%. Z-scores differed by approximately 0.2 and this was statistically significant, although the significance of this difference diminished when controlling for Year 4 results. Students who acted as PAL tutors who had scored in the top quartile in Year 4 examinations scored significantly better in a long-station objective structured clinical examination (LSO). Although students who acted as PAL tutors performed better than students who did not in final-year examinations, this difference was small and attributable to the students' background academic abilities. High-achieving students appear to be self-selecting as peer-tutors and their enhanced performance in LSOs may reflect their

  8. Peak and ceiling effects in final-product analysis of mastoidectomy performance

    DEFF Research Database (Denmark)

    West, N; Konge, L; Cayé-Thomasen, P

    2015-01-01

    BACKGROUND: Virtual reality surgical simulation of mastoidectomy is a promising training tool for novices. Final-product analysis for assessing novice mastoidectomy performance could be limited by a peak or ceiling effect. These may be countered by simulator-integrated tutoring. METHODS: Twenty......-two participants completed a single session of self-directed practice of the mastoidectomy procedure in a virtual reality simulator. Participants were randomised for additional simulator-integrated tutoring. Performances were assessed at 10-minute intervals using final-product analysis. RESULTS: In all, 45.5 per...... cent of participants peaked before the 60-minute time limit. None of the participants achieved the maximum score, suggesting a ceiling effect. The tutored group performed better than the non-tutored group but tutoring did not eliminate the peak or ceiling effects. CONCLUSION: Timing and adequate...

  9. Student-written single-best answer questions predict performance in finals.

    Science.gov (United States)

    Walsh, Jason; Harris, Benjamin; Tayyaba, Saadia; Harris, David; Smith, Phil

    2016-10-01

    Single-best answer (SBA) questions are widely used for assessment in medical schools; however, often clinical staff have neither the time nor the incentive to develop high-quality material for revision purposes. A student-led approach to producing formative SBA questions offers a potential solution. Cardiff University School of Medicine students created a bank of SBA questions through a previously described staged approach, involving student question-writing, peer-review and targeted senior clinician input. We arranged questions into discrete tests and posted these online. Student volunteer performance on these tests from the 2012/13 cohort of final-year medical students was recorded and compared with the performance of these students in medical school finals (knowledge and objective structured clinical examinations, OSCEs). In addition, we compared the performance of students that participated in question-writing groups with the performance of the rest of the cohort on the summative SBA assessment. Often clinical staff have neither the time nor the incentive to develop high-quality material for revision purposes Performance in the end-of-year summative clinical knowledge SBA paper correlated strongly with performance in the formative student-written SBA test (r = ~0.60, p performance in end-of-year SBA examinations, and therefore can provide a potentially valuable revision resource. There is potential for student-written questions to be incorporated into summative examinations. © 2015 John Wiley & Sons Ltd.

  10. Assessment of estrogen receptor-monoclonal antibody interaction by high performance liquid chromatography

    International Nuclear Information System (INIS)

    Brandt, D.W.; Wittliff, J.L.

    1986-01-01

    To define the interrelationships between the various isoforms of the estrogen receptors (ER), a monoclonal antibody-horse radish peroxidase conjugate H222 was used as a probe in conjunction with HPIEC (Synchrom AX-1000) and HPSEC (TSK-3000 SW Toyo Soda) columns. ER from breast tumors was assessed using [16α- 125 I]-iodoestradiol-17β (3nM) +/-200 fold excess estradiol-17β and excess H222. When ER was analyzed by HPSEC (size and shape), with 400 mM KCl which caused the dissociation of ER into 4S isoforms, a shift in retension time to higher molecular weight species was seen. The H222 appeared to interact with most isoforms of ER. However, when ER was analyzed by HPIEC (surface charge) with H222, a shift in virtually all of the high salt (150mM) isoform to the flow-through was observed with only 46% shift in elution of the low salt (60-70mM) isoforms. H222 did not alter total ER binding capacity. These data suggest that H222 recognized discrete forms of the ER. Therefore, modification in the receptor may have occurred which masks or removes the antigenic determinant limiting the specificity of H222. These results indicate that H222 may be employed as a tool to elucidate the interrelationships between these ER species

  11. Performance evaluation of phage-displayed synthetic human single-domain antibody libraries: A retrospective analysis.

    Science.gov (United States)

    Henry, Kevin A; Tanha, Jamshid

    2018-05-01

    Fully human synthetic single-domain antibodies (sdAbs) are desirable therapeutic molecules but their development is a considerable challenge. Here, using a retrospective analysis of in-house historical data, we examined the parameters that impact the outcome of screening phage-displayed synthetic human sdAb libraries to discover antigen-specific binders. We found no evidence for a differential effect of domain type (V H or V L ), library randomization strategy, incorporation of a stabilizing disulfide linkage or sdAb display format (monovalent vs. multivalent) on the probability of obtaining any antigen-binding human sdAbs, instead finding that the success of library screens was primarily related to properties of target antigens, especially molecular mass. The solubility and binding affinity of sdAbs isolated from successful screens depended both on properties of the sdAb libraries (primarily domain type) and the target antigens. Taking attrition of sdAbs with major manufacturability concerns (aggregation; low expression) and sdAbs that do not recognize native cell-surface antigens as independent probabilities, we calculate the overall likelihood of obtaining ≥1 antigen-binding human sdAb from a single library-target screen as ~24%. Successful library-target screens should be expected to yield ~1.3 human sdAbs on average, each with average binding affinity of ~2 μM. Copyright © 2018 Elsevier B.V. All rights reserved.

  12. Silica final lens performance in laser fusion facilities: HiPER and LIFE

    OpenAIRE

    Garoz, D.; Gonzalez Arrabal, Raquel; Juarez Mañas, Rafael; Alvarez Ruiz, Jesus; Sanz, J.; Perlado Martín, José Manuel; Rivera de Mena, Antonio

    2012-01-01

    Nowadays, the projects LIFE (Laser Inertial Fusion Energy) in USA and HiPER (High Power Laser Energy Research) in Europe are the most advanced ones to demonstrate laser fusion energy viability. One of the main points of concern to properly achieve ignition is the performance of the final optics (lenses) under the severe irradiation conditions that take place in fusion facilities. In this paper, we calculate the radiation fluxes and doses as well as the radiation-induced temperature enha...

  13. The Institute for Sustained Performance, Energy, and Resilience, University of North Carolina, Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Fowler, Robert [Univ. of North Carolina, Chapel Hill, NC (United States)

    2018-01-20

    This is the final report for the UNC component of the SciDAD Institute for Sustained Performance, Energy, and Resilience. In this report, we describe activities on the SUPER project at RENCI at the University of North Carolina at Chapel Hill. While we focus particularly on UNC, we touch on project-wide activities as well as, on interactions with, and impacts on, other projects.

  14. Frequency of chest pain in primary care, diagnostic tests performed and final diagnoses.

    Science.gov (United States)

    Hoorweg, Beatrijs Bn; Willemsen, Robert Ta; Cleef, Lotte E; Boogaerts, Tom; Buntinx, Frank; Glatz, Jan Fc; Dinant, Geert Jan

    2017-11-01

    Observational study of patients with chest pain in primary care: determination of incidence, referral rate, diagnostic tests and (agreement between) working and final diagnoses. 118 general practitioners (GPs) in the Netherlands and Belgium recorded all patient contacts during  2weeks. Furthermore, patients presenting with chest pain were registered extensively. A follow-up form was filled in after 30 days. 22 294 patient contacts were registered. In 281 (1.26%), chest pain was a reason for consulting the GP (mean age for men 54.4/women 53 years). In this cohort of 281 patients, in 38.1% of patients, acute coronary syndrome (ACS) was suspected at least temporarily during consultation, 40.2% of patients were referred to secondary care and 512 diagnostic tests were performed by GPs and consulted specialists. Musculoskeletal pain was the most frequent working (26.1%) and final diagnoses (33.1%). Potentially life-threatening diseases as final diagnosis (such as myocardial infarction) accounted for 8.4% of all chest pain cases. In 23.1% of cases, a major difference between working and final diagnoses was found, in 0.7% a severe disease was initially missed by the GP. Chest pain was present in 281 patients (1.26% of all consultations). Final diagnoses were mostly non-life-threatening. Nevertheless, in 8.4% of patients with chest pain, life-threatening underlying causes were identified. This seems reflected in the magnitude and wide variety of diagnostic tests performed in these patients by GPs and specialists, in the (safe) overestimation of life-threatening diseases by GPs at initial assessment and in the high referral rate we found. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  15. Process performance and product quality in an integrated continuous antibody production process.

    Science.gov (United States)

    Karst, Daniel J; Steinebach, Fabian; Soos, Miroslav; Morbidelli, Massimo

    2017-02-01

    Continuous manufacturing is currently being seriously considered in the biopharmaceutical industry as the possible new paradigm for producing therapeutic proteins, due to production cost and product quality related benefits. In this study, a monoclonal antibody producing CHO cell line was cultured in perfusion mode and connected to a continuous affinity capture step. The reliable and stable integration of the two systems was enabled by suitable control loops, regulating the continuous volumetric flow and adapting the operating conditions of the capture process. For the latter, an at-line HPLC measurement of the harvest concentration subsequent to the bioreactor was combined with a mechanistic model of the capture chromatographic unit. Thereby, optimal buffer consumption and productivity throughout the process was realized while always maintaining a yield above the target value of 99%. Stable operation was achieved at three consecutive viable cell density set points (20, 60, and 40 × 10 6 cells/mL), together with consistent product quality in terms of aggregates, fragments, charge isoforms, and N-linked glycosylation. In addition, different values for these product quality attributes such as N-linked glycosylation, charge variants, and aggregate content were measured at the different steady states. As expected, the amount of released DNA and HCP was significantly reduced by the capture step for all considered upstream operating conditions. This study is exemplary for the potential of enhancing product quality control and modulation by integrated continuous manufacturing. Biotechnol. Bioeng. 2017;114: 298-307. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  16. Clinical observed performance evaluation: a prospective study in final year students of surgery.

    LENUS (Irish Health Repository)

    Markey, G C

    2010-06-24

    We report a prospective study of clinical observed performance evaluation (COPE) for 197 medical students in the pre-qualification year of clinical education. Psychometric quality was the main endpoint. Students were assessed in groups of 5 in 40-min patient encounters, with each student the focus of evaluation for 8 min. Each student had a series of assessments in a 25-week teaching programme. Over time, several clinicians from a pool of 16 surgical consultants and registrars evaluated each student by direct observation. A structured rating form was used for assessment data. Variance component analysis (VCA), internal consistency and inter-rater agreement were used to estimate reliability. The predictive and convergent validity of COPE in relation to summative OSCE, long case, and overall final examination was estimated. Median number of COPE assessments per student was 7. Generalisability of a mean score over 7 COPE assessments was 0.66, equal to that of an 8 x 7.5 min station final OSCE. Internal consistency was 0.88-0.97 and inter-rater agreement 0.82. Significant correlations were observed with OSCE performance (R = 0.55 disattenuated) and long case (R = 0.47 disattenuated). Convergent validity was 0.81 by VCA. Overall final examination performance was linearly related to mean COPE score with standard error 3.7%. COPE permitted efficient serial assessment of a large cohort of final year students in a real world setting. Its psychometric quality compared well with conventional assessments and with other direct observation instruments as reported in the literature. Effect on learning, and translation to clinical care, are directions for future research.

  17. Silica final lens performance in laser fusion facilities: HiPER and LIFE

    Science.gov (United States)

    Garoz, D.; González-Arrabal, R.; Juárez, R.; Álvarez, J.; Sanz, J.; Perlado, J. M.; Rivera, A.

    2013-01-01

    Nowadays, the projects LIFE (Laser Inertial Fusion Energy) in USA and HiPER (High Power Laser Energy Research) in Europe are the most advanced ones to demonstrate laser fusion energy viability. One of the main points of concern to properly achieve ignition is the performance of the final optics (lenses) under the severe irradiation conditions that take place in fusion facilities. In this paper, we calculate the radiation fluxes and doses as well as the radiation-induced temperature enhancement and colour centre formation in final lenses assuming realistic geometrical configurations for HiPER and LIFE. On these bases, the mechanical stresses generated by the established temperature gradients are evaluated showing that from a mechanical point of view lenses only fulfil specifications if ions resulting from the imploding target are mitigated. The absorption coefficient of the lenses is calculated during reactor startup and steady-state operation. The obtained results reveal the necessity of new solutions to tackle ignition problems during the startup process for HiPER. Finally, we evaluate the effect of temperature gradients on focal length changes and lens surface deformations. In summary, we discuss the capabilities and weak points of silica lenses and propose alternatives to overcome predictable problems.

  18. Performance of three microimmunofluorescence assays for detection of Chlamydia pneumoniae immunoglobulin M, G, and A antibodies

    DEFF Research Database (Denmark)

    Bennedsen, Mette; Berthelsen, Lene; Lind, Inga

    2002-01-01

    Labsystems (LAB) and MRL Diagnostics (MRL) by investigation of sera from three groups of patients: group I, 83 sera from 28 patients with atypical pneumonia; group II, 37 sera from 16 patients with acute C. pneumoniae or Chlamydia psittaci respiratory tract infection confirmed by PCR or culture; group III......The microimmunofluorescence (MIF) test is considered the "gold standard" for laboratory diagnosis of acute and chronic Chlamydia pneumoniae infection. The performance of a MIF test based on C. pneumoniae antigen from Washington Research Foundation (WRF) was compared with those of assays from...

  19. Monoclonal antibodies and cancer

    International Nuclear Information System (INIS)

    Haisma, H.J.

    1987-01-01

    The usefulness of radiolabeled monoclonal antibodies for imaging and treatment of human (ovarian) cancer was investigated. A review of tumor imaging with monoclonal antibodies is presented. Special attention is given to factors that influence the localization of the antibodies in tumors, isotope choice and methods of radiolabeling of the monoclonal antibodies. Two monoclonal antibodies, OC125 and OV-TL3, with high specificity for human epithelial ovarian cancer are characterized. A simple radio-iodination technique was developed for clinical application of the monoclonal antibodies. The behavior of monoclonal antibodies in human tumor xenograft systems and in man are described. Imaging of tumors is complicated because of high background levels of radioactivity in other sites than the tumor, especially in the bloodpool. A technique was developed to improve imaging of human tumor xenographs in nude mice, using subtraction of a specific and a non-specific antibody, radiolabeled with 111 In, 67 Ga and 131 I. To investigate the capability of the two monoclonal antibodies, to specifically localize in human ovarian carcinomas, distribution studies in mice bearing human ovarian carcinoma xenografts were performed. One of the antibodies, OC125, was used for distribution studies in ovarian cancer patients. OC125 was used because of availability and approval to use this antibody in patients. The same antibody was used to investigate the usefulness of radioimmunoimaging in ovarian cancer patients. The interaction of injected radiolabeled antibody OC125 with circulating antigen and an assay to measure the antibody response in ovarian cancer patients after injection of the antibody is described. 265 refs.; 30 figs.; 19 tabs

  20. Performance Evaluation of a Novel Chemiluminescence Assay Detecting Treponema Pallidum Antibody as a Syphilis Screening Method.

    Science.gov (United States)

    Chen, Qixia; An, Jingna; Rao, Chenli; Wang, Tingting; Li, Dongdong; Feng, Shu; Tao, Chuanmin

    2016-01-01

    Syphilis is a major concern to global public health with increasing incidence. So its screening test should have sufficient sensitivity and specificity. We evaluated the performance of the Lumipulse G TP-N assay detection for syphilis screening and compared it with the InTec ELISA test kit for TP, which is widely used. Samples of several patient groups including 133 clinical and serologically characterized syphilitic sera, 175 samples containing potentially interfering agents, and 2290 unselected samples submitted for routine screening were detected by both the Lumipulse G TP-N assay and the InTec ELISA test kit for TP. Inconsistent samples were confirmed by RecomLine Treponema IgG, IgM immunoblot. Coefficient of variations of the Lumipulseo G TP-N assay at both levels were below 5% and of the InTec ELISA test kit for TP both over 5%. The sensitivity of the Lumipulse G TP-N assay and the InTec ELISA test kit for TP were 100% for all stages of syphilis. The two methods had consistent analytical specificity of 100% (95% CI: 97.21 - 100.00), while the clinical specificity was 100% (95% CI: 99.79 - 100.00) and 99.82% (95% CI: 99.51 - 99.94), respectively. Between them, Spearman's correlation coefficient was 0.455 and kappa value was 0.986. The overall sensitivity and specificity of the Lumipulse G TP-N assay was higher than the InTec ELISA test kit for TP (sensitivity: 100.0 versus 99.5, specificity: 100.0 versus 99.8). The automated Lumipulse G TP-N assay demonstrated excellent diagnostic sensitivity and specificity when evaluated as a screening test for syphilis. Thus, it can be an alternative to the treponemal screening test.

  1. Final waste forms project: Performance criteria for phase I treatability studies

    International Nuclear Information System (INIS)

    Gilliam, T.M.; Hutchins, D.A.; Chodak, P. III

    1994-06-01

    This document defines the product performance criteria to be used in Phase I of the Final Waste Forms Project. In Phase I, treatability studies will be performed to provide open-quotes proof-of-principleclose quotes data to establish the viability of stabilization/solidification (S/S) technologies. This information is required by March 1995. In Phase II, further treatability studies, some at the pilot scale, will be performed to provide sufficient data to allow treatment alternatives identified in Phase I to be more fully developed and evaluated, as well as to reduce performance uncertainties for those methods chosen to treat a specific waste. Three main factors influence the development and selection of an optimum waste form formulation and hence affect selection of performance criteria. These factors are regulatory, process-specific, and site-specific waste form standards or requirements. Clearly, the optimum waste form formulation will require consideration of performance criteria constraints from each of the three categories. Phase I will focus only on the regulatory criteria. These criteria may be considered the minimum criteria for an acceptable waste form. In other words, a S/S technology is considered viable only if it meet applicable regulatory criteria. The criteria to be utilized in the Phase I treatability studies were primarily taken from Environmental Protection Agency regulations addressed in 40 CFR 260 through 265 and 268; and Nuclear Regulatory Commission regulations addressed in 10 CFR 61. Thus the majority of the identified criteria are independent of waste form matrix composition (i.e., applicable to cement, glass, organic binders etc.)

  2. Final waste forms project: Performance criteria for phase I treatability studies

    Energy Technology Data Exchange (ETDEWEB)

    Gilliam, T.M. [Oak Ridge National Lab., TN (United States); Hutchins, D.A. [Martin Marietta Energy Systems, Inc., Oak Ridge, TN (United States); Chodak, P. III [Massachusetts Institute of Technology (United States)

    1994-06-01

    This document defines the product performance criteria to be used in Phase I of the Final Waste Forms Project. In Phase I, treatability studies will be performed to provide {open_quotes}proof-of-principle{close_quotes} data to establish the viability of stabilization/solidification (S/S) technologies. This information is required by March 1995. In Phase II, further treatability studies, some at the pilot scale, will be performed to provide sufficient data to allow treatment alternatives identified in Phase I to be more fully developed and evaluated, as well as to reduce performance uncertainties for those methods chosen to treat a specific waste. Three main factors influence the development and selection of an optimum waste form formulation and hence affect selection of performance criteria. These factors are regulatory, process-specific, and site-specific waste form standards or requirements. Clearly, the optimum waste form formulation will require consideration of performance criteria constraints from each of the three categories. Phase I will focus only on the regulatory criteria. These criteria may be considered the minimum criteria for an acceptable waste form. In other words, a S/S technology is considered viable only if it meet applicable regulatory criteria. The criteria to be utilized in the Phase I treatability studies were primarily taken from Environmental Protection Agency regulations addressed in 40 CFR 260 through 265 and 268; and Nuclear Regulatory Commission regulations addressed in 10 CFR 61. Thus the majority of the identified criteria are independent of waste form matrix composition (i.e., applicable to cement, glass, organic binders etc.).

  3. 7X performance results - final report : ASCI Red vs Red Storm.

    Energy Technology Data Exchange (ETDEWEB)

    Dinge, Dennis C. (Cray Inc., Albuquerque, NM); Davis, Michael E. (Cray Inc., Albuquerque, NM); Haskell, Karen H.; Ballance, Robert A.; Gardiner, Thomas Anthony; Stevenson, Joel O.; Noe, John P.

    2011-04-01

    The goal of the 7X performance testing was to assure Sandia National Laboratories, Cray Inc., and the Department of Energy that Red Storm would achieve its performance requirements which were defined as a comparison between ASCI Red and Red Storm. Our approach was to identify one or more problems for each application in the 7X suite, run those problems at multiple processor sizes in the capability computing range, and compare the results between ASCI Red and Red Storm. The first part of this report describes the two computer systems, the applications in the 7X suite, the test problems, and the results of the performance tests on ASCI Red and Red Storm. During the course of the testing on Red Storm, we had the opportunity to run the test problems in both single-core mode and dual-core mode and the second part of this report describes those results. Finally, we reflect on lessons learned in undertaking a major head-to-head benchmark comparison.

  4. Planning for risk-informed/performance-based fire protection at nuclear power plants. Final report

    International Nuclear Information System (INIS)

    Najafi, B.; Parkinson, W.J.; Lee, J.A.

    1997-12-01

    This document presents a framework for discussing issues and building consensus towards use of fire modeling and risk technology in nuclear power plant fire protection program implementation. The plan describes a three-phase approach: development of core technologies, implementation of methods, and finally, case studies and pilot applications to verify viability of such methods. The core technologies are defined as fire modeling, fire and system tests, use of operational data, and system and risk techniques. The implementation phase addresses the programmatic issues involved in implementing a risk-informed/performance-based approach in an integrated approach with risk/performance measures. The programmatic elements include: (1) a relationship with fire codes and standards development as defined by the ongoing effort of NFPA for development of performance-based standards; (2) the ability for NRC to undertake inspection and enforcement; and (3) the benefit to utilities in terms of cost versus safety. The case studies are intended to demonstrate applicability of single issue resolution while pilot applications are intended to check the applicability of the integrated program as a whole

  5. IMPER: Characterization of the wind field over a large wind turbine rotor - final report; Improved performance

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt Paulsen, U.; Wagner, R.

    2012-01-15

    A modern wind turbine rotor with a contemporary rotor size would easily with the tips penetrate the air between 116 m and 30 m and herby experience effects of different wind. With current rules on power performance measurements such as IEC 61400-121 the reference wind speed is measured at hub height, an oversimplification of the wind energy power over the rotor disk area is carried out. The project comprised a number of innovative and coordinated measurements on a full scale turbine with remote sensing technology and simulations on a 500 kW wind turbine for the effects of wind field characterization. The objective with the present report is to give a short overview of the different experiments carried out and results obtained within the final phase of this project. (Author)

  6. Final report of the project performance assessment and economic evaluation of nuclear waste management

    International Nuclear Information System (INIS)

    Rasilainen, K.; Anttila, M.; Hautojaervi, A.

    1993-05-01

    The publication is the final report of project Performance Assessment and Economic Evaluation of Nuclear Waste Management (TOKA) at the Nuclear Engineering Laboratory of VTT (Technical Research Centre of Finland), forming part of the Publicly Financed Nuclear Waste Management Research Programme (JYT). The project covers safety and cost aspects of all phases of nuclear waste management. The main emphasis has been on developing an integrated system of models for performance assessment of nuclear waste repositories. During the four years the project has so far been in progress, the total amount of work has been around 14 person-years. Computer codes are the main tools in the project, they are either developed by the project team or acquired from abroad. In-house model development has been especially active in groundwater flow, near-field and migration modelling. The quantitative interpretation of Finnish tracer experiments in the laboratory and natural analogue studies at Palmottu support performance assessments via increased confidence in the migration concepts used. The performance assessment philosophy adopted by the team consists of deterministic modelling and pragmatic scenario analysis. This is supported by the long-term experience in practical performance assessment of the team, and in theoretical probabilistic modelling exercises. The radiological risks of spent fuel transportation from the Loviisa nuclear power plant to Russia have been analysed using a probabilistic computer code and Finnish traffic accident statistics. The project assists the authorities in the annual assessment of utility estimates of funding needs for future nuclear waste management operations. The models and methods used within the project are tested in international verification/validation projects

  7. Effects of NH3 PECVD treatment time on the performance of multiwall carbon nanotubes for antibody immobilization.

    Science.gov (United States)

    Hu, Chih-Chung; Chiou, Ai-Huei; Hsu, Chun-Yao

    2016-10-01

    This work presents an amino plasma-enhanced chemical vapour deposition (NH3 PECVD) treatment to modify multiwall carbon nanotubes (MWCNTs) for the immobilization of antibodies (IgG-gold), and the effects of treatment time were studied. A titanium nitride (TiN) buffer layer and a nickel catalyst layer were deposited on silicon substrates for synthesis of MWCNTs using thermal CVD. The MWCNTs were modified by amine (NH2 (-) ) or amino (NH(-) ) functional groups for 1, 3, and 5 min by PECVD, respectively. Mouse IgG-golds were immobilized on the modified MWCNTs using glutaraldehyde (GA) as a crosslinker. The performance of the modified MWCNTs was characterized by scanning electron microscope (SEM), energy dispersive spectrometer (EDS), Fourier transform infrared spectroscopy (FT-IR), contact angle system (CA), X-ray photoelectron spectroscopy (XPS, ESCA), and UV-visible spectroscopy. Results show that the efficiency of IgG-gold immobilization was increased with the increase of NH3 PECVD treatment time. The NH3 PECVD treatment changed the surface properties of the MWCNTs, tuning them more hydrophilic without affecting their structure. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1343-1351, 2016. © 2015 Wiley Periodicals, Inc.

  8. Antibodies to phosphatidylserine/prothrombin (aPS/PT) enhanced the diagnostic performance in Chinese patients with antiphospholipid syndrome.

    Science.gov (United States)

    Zhang, Shulan; Wu, Ziyan; Zhang, Wen; Zhao, Jiuliang; Norman, Gary L; Zeng, Xiaofeng; Zhang, Fengchun; Li, Yongzhe

    2018-03-21

    Increasing evidence has highlighted the role of non-criteria antiphospholipid antibodies (aPLs) as important supplements to the current criteria aPLs for the diagnosis of antiphospholipid syndrome (APS). In this retrospective study, we evaluated the clinical relevance of antibodies to phosphatidylserine/prothrombin (aPS/PT) in Chinese patients with APS. A total of 441 subjects were tested, including 101 patients with primary APS (PAPS), 140 patients with secondary APS (SAPS), 161 disease controls (DCs) and 39 healthy controls (HCs). Serum IgG/IgM aPS/PT was determined by ELISA. The levels of IgG/IgM aPS/PT were significantly increased in patients with APS compared with DCs and HCs. IgG and IgM aPS/PT were present in 29.7% and 54.5% of PAPS, and 42.1% and 53.6% of SAPS, respectively. For diagnosis of APS, IgG aCL exhibited the highest positive likelihood ratio (LR+) of 21.60, followed by LA (13.84), IgG aβ2GP1 (9.19) and IgG aPS/PT (8.49). aPS/PT was detected in 13.3% of seronegative PAPS patients and 31.3% of seronegative SAPS patients. LA exhibited the highest OR of 3.64 in identifying patients with thrombosis, followed by IgG aCL (OR, 2.63), IgG aPS/PT (OR, 2.55) and IgG aβ2GP1 (OR, 2.33). LA and IgG aCL were correlated with both arterial and venous thrombosis, whereas IgG aPS/PT and IgG aβ2GP1 correlated with venous or arterial thrombosis, respectively. Our findings suggest that the inclusion of IgG/IgM aPS/PT may enhance the diagnostic performance for APS, especially in those in whom APS is highly suspected, but conventional aPLs are repeatedly negative. In addition, IgG aPS/PT may contribute to identify patients at risk of thrombosis.

  9. Labelling, quality control and clinical evaluation of monoclonal antibodies for scintigraphy. Final report of a co-ordinated research programme 1991-1996

    International Nuclear Information System (INIS)

    1998-03-01

    Realizing the potential of labelled monoclonal antibodies for in vivo diagnosis and therapy and the interest in many developing Member States for acquiring expertise in this field the IAEA initiated a co-ordinated research programme in 1991 focusing on 99 Tc m labelling of antibodies, their quality control and scintigraphic evaluation. Twelve laboratories from Asia, Latin America, Europe and North America participated in this programme which was concluded in 1996. During this programme the participants investigated the 99 Tc m labelling of a murine anti-CEA antibody using the method of chelating 99 Tc m with the free sulfhydryl groups generated by reaction with reducing agents such as mercapto ethanol. During the later part of the programme this method was also extended to 99 Tc m labelling of hIgG. All the participating laboratories could gain valuable experience in 99 Tc m antibody labelling techniques and formulation of kits. Many of them have been use in patients by collaborating nuclear medicine specialists with satisfactory results. This report is a compilation of the detailed results obtained by the participating laboratories and includes a summary and assessment of the achievement of the CRP

  10. Assessment of the effects of glutamic acid decarboxylase antibodies and trace elements on cognitive performance in older adults

    Directory of Open Access Journals (Sweden)

    Alghadir AH

    2015-12-01

    Full Text Available Ahmad H Alghadir,1 Sami A Gabr,1,2 Einas Al-Eisa11Department of Rehabilitation Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia; 2Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura, EgyptBackground: Homeostatic imbalance of trace elements such as iron (Fe, copper (Cu, and zinc (Zn demonstrated adverse effects on brain function among older adults.Objective: The present study aimed to investigate the effects of trace elements and the presence of anti-glutamic acid decarboxylase antibodies (GADAs in human cognitive abilities among healthy older adults.Methods: A total of 100 healthy subjects (65 males, 35 females; age range; 64–96 years were recruited for this study. Based on Loewenstein Occupational Therapy Cognitive Assessment (LOTCA score, the participants were classified according to cognitive performance into normal (n=45, moderate (n=30, and severe (n=25. Cognitive functioning, leisure-time physical activity (LTPA, serum trace elements – Fe, Cu, Zn, Zn/Cu, and GADAs were assessed using LOTCA battery, pre-validated physical activity (PA questionnaire, atomic absorption, and immunoassay techniques, respectively.Results: Approximately 45% of the study population (n=45 had normal distribution of cognitive function and 55% of the study population (n=55 had abnormal cognitive function; they were classified into moderate (score 62–92 and severe (score 31–62. There was a significant reduction in the level of Zn and Zn/Cu ratio along with an increase in the level of Fe, Cu, and anti-GADAs in subjects of severe (P=0.01 and moderate (P=0.01 cognitive performance. LOTCA-cognitive scores correlated positively with sex, HbA1c, Fe, Cu, Zn, and Zn/Cu ratio, and negatively with age, PA, body mass index, and anti-GADAs. Significant inter-correlation was reported between serum trace element concentrations and anti-GADAs which suggest producing a cognitive decline via oxidative and neural

  11. Monoclonal antibody heterogeneity analysis and deamidation monitoring with high-performance cation-exchange chromatofocusing using simple, two component buffer systems.

    Science.gov (United States)

    Kang, Xuezhen; Kutzko, Joseph P; Hayes, Michael L; Frey, Douglas D

    2013-03-29

    The use of either a polyampholyte buffer or a simple buffer system for the high-performance cation-exchange chromatofocusing of monoclonal antibodies is demonstrated for the case where the pH gradient is produced entirely inside the column and with no external mixing of buffers. The simple buffer system used was composed of two buffering species, one which becomes adsorbed onto the column packing and one which does not adsorb, together with an adsorbed ion that does not participate in acid-base equilibrium. The method which employs the simple buffer system is capable of producing a gradual pH gradient in the neutral to acidic pH range that can be adjusted by proper selection of the starting and ending pH values for the gradient as well as the buffering species concentration, pKa, and molecular size. By using this approach, variants of representative monoclonal antibodies with isoelectric points of 7.0 or less were separated with high resolution so that the approach can serve as a complementary alternative to isoelectric focusing for characterizing a monoclonal antibody based on differences in the isoelectric points of the variants present. Because the simple buffer system used eliminates the use of polyampholytes, the method is suitable for antibody heterogeneity analysis coupled with mass spectrometry. The method can also be used at the preparative scale to collect highly purified isoelectric variants of an antibody for further study. To illustrate this, a single isoelectric point variant of a monoclonal antibody was collected and used for a stability study under forced deamidation conditions. Copyright © 2013 Elsevier B.V. All rights reserved.

  12. Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification.

    Science.gov (United States)

    Mendoza-Parra, Marco-Antonio; Saravaki, Vincent; Cholley, Pierre-Etienne; Blum, Matthias; Billoré, Benjamin; Gronemeyer, Hinrich

    2016-01-01

    We have established a certification system for antibodies to be used in chromatin immunoprecipitation assays coupled to massive parallel sequencing (ChIP-seq). This certification comprises a standardized ChIP procedure and the attribution of a numerical quality control indicator (QCi) to biological replicate experiments. The QCi computation is based on a universally applicable quality assessment that quantitates the global deviation of randomly sampled subsets of ChIP-seq dataset with the original genome-aligned sequence reads. Comparison with a QCi database for >28,000 ChIP-seq assays were used to attribute quality grades (ranging from 'AAA' to 'DDD') to a given dataset. In the present report we used the numerical QC system to assess the factors influencing the quality of ChIP-seq assays, including the nature of the target, the sequencing depth and the commercial source of the antibody.  We have used this approach specifically to certify mono and polyclonal antibodies obtained from Active Motif directed against the histone modification marks H3K4me3, H3K27ac and H3K9ac for ChIP-seq. The antibodies received the grades AAA to BBC ( www.ngs-qc.org). We propose to attribute such quantitative grading of all antibodies attributed with the label "ChIP-seq grade".

  13. Investigation of modern methods of probalistic sensitivity analysis of final repository performance assessment models (MOSEL)

    International Nuclear Information System (INIS)

    Spiessl, Sabine; Becker, Dirk-Alexander

    2017-06-01

    Sensitivity analysis is a mathematical means for analysing the sensitivities of a computational model to variations of its input parameters. Thus, it is a tool for managing parameter uncertainties. It is often performed probabilistically as global sensitivity analysis, running the model a large number of times with different parameter value combinations. Going along with the increase of computer capabilities, global sensitivity analysis has been a field of mathematical research for some decades. In the field of final repository modelling, probabilistic analysis is regarded a key element of a modern safety case. An appropriate uncertainty and sensitivity analysis can help identify parameters that need further dedicated research to reduce the overall uncertainty, generally leads to better system understanding and can thus contribute to building confidence in the models. The purpose of the project described here was to systematically investigate different numerical and graphical techniques of sensitivity analysis with typical repository models, which produce a distinctly right-skewed and tailed output distribution and can exhibit a highly nonlinear, non-monotonic or even non-continuous behaviour. For the investigations presented here, three test models were defined that describe generic, but typical repository systems. A number of numerical and graphical sensitivity analysis methods were selected for investigation and, in part, modified or adapted. Different sampling methods were applied to produce various parameter samples of different sizes and many individual runs with the test models were performed. The results were evaluated with the different methods of sensitivity analysis. On this basis the methods were compared and assessed. This report gives an overview of the background and the applied methods. The results obtained for three typical test models are presented and explained; conclusions in view of practical applications are drawn. At the end, a recommendation

  14. Investigation of modern methods of probalistic sensitivity analysis of final repository performance assessment models (MOSEL)

    Energy Technology Data Exchange (ETDEWEB)

    Spiessl, Sabine; Becker, Dirk-Alexander

    2017-06-15

    Sensitivity analysis is a mathematical means for analysing the sensitivities of a computational model to variations of its input parameters. Thus, it is a tool for managing parameter uncertainties. It is often performed probabilistically as global sensitivity analysis, running the model a large number of times with different parameter value combinations. Going along with the increase of computer capabilities, global sensitivity analysis has been a field of mathematical research for some decades. In the field of final repository modelling, probabilistic analysis is regarded a key element of a modern safety case. An appropriate uncertainty and sensitivity analysis can help identify parameters that need further dedicated research to reduce the overall uncertainty, generally leads to better system understanding and can thus contribute to building confidence in the models. The purpose of the project described here was to systematically investigate different numerical and graphical techniques of sensitivity analysis with typical repository models, which produce a distinctly right-skewed and tailed output distribution and can exhibit a highly nonlinear, non-monotonic or even non-continuous behaviour. For the investigations presented here, three test models were defined that describe generic, but typical repository systems. A number of numerical and graphical sensitivity analysis methods were selected for investigation and, in part, modified or adapted. Different sampling methods were applied to produce various parameter samples of different sizes and many individual runs with the test models were performed. The results were evaluated with the different methods of sensitivity analysis. On this basis the methods were compared and assessed. This report gives an overview of the background and the applied methods. The results obtained for three typical test models are presented and explained; conclusions in view of practical applications are drawn. At the end, a recommendation

  15. Calculation method for the seasonal performance of heat pump compact units and validation. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Wemhoener, C.; Dott, R.; Afjei, Th. [University of Applied Sciences Northwestern Switzerland, Institute of Energy in Buildings, Muttenz (Switzerland); Huber, H.; Helfenfinger, D.; Keller, P.; Furter, R. [University of Applied Sciences Lucerne (HTA), Test center HLKS, Horw (Switzerland)

    2007-02-15

    This comprehensive final report for the Swiss Federal Office of Energy (SFOE) takes a look at compact heat pump units that have been developed for the heating of low energy consumption houses built to MINERGIE or MINERGIE-P standards. These units, which combine the functions of space heating, domestic hot water preparation and ventilation in one unit are described. A testing procedure developed at the University of Applied Science in Lucerne, Switzerland, using a test rig for the measurement of the seasonal performance factor (SPF) is described. A calculation method based on temperature classes for the calculation of the SPF of combined heat pump systems for space heating and domestic hot water preparation that was developed by the Institute of Energy in Buildings at the University of Applied Sciences Northwestern Switzerland is examined. Two pilot plants allowing detailed field monitoring of two compact units are described. One pilot plant installed in a single-family house built to MINERGIE standard in Gelterkinden, Switzerland, provided data on a compact unit. These results of measurements made on this and a further installation in a MINERGIE-P ultra-low energy consumption house in Zeiningen, Switzerland, are presented and discussed. Calculation methods, including exergy considerations are reviewed and their validation is discussed.

  16. Knowledge Base for Strainer Clogging - Modifications performed in different countries since 1992 - Final report

    International Nuclear Information System (INIS)

    2002-01-01

    performed mock-up studies. Full-scale tests were also performed in the condensation pool of some units. The experiment results were utilised for the optimisation in most BWRs of new strainer designs, and for the choice of new thermal insulation materials. The results obtained confirmed, mostly, the appropriate design of existing PWR strainers. PWR and Candu reactors related s studies and experiments are, however, still on-going in a few countries. The modifications of the ECCS and/or CSS suction strainers have been performed after the aforementioned exhaustive studies and experiments. The modifications have resulted in new strainer designs with significantly enlarged filtering area. Most of the new strainers have good self-cleaning properties. Relatively many of them have been provided with instrumentation for differential pressure measurement, with indications and alarms in the unit main control room, and at some units in the emergency control room. In some BWRs, the design encompasses the possibility to back-flush the strainers. Replacements of large fractions of the thermal insulation materials utilised on piping and other components inside the containment have taken place. The newly installed insulation materials vary both within and between countries. They are primarily RMI (Reflecting Metallic Insulation), nuclear grade fiberglass, mineral wool and calcium silicate. The same insulation material - for example mineral wool - can be installed differently in different countries, i.e. jacketed or encapsulated in cassettes. The results of the experiments have significantly contributed to the materials selected or installation methods. The administrative measures taken by plant owners include, e.g. a periodic cleanup of the suppression pool and the containment sumps, with the aim to minimise the presence of foreign materials, and the control and eventual betterment of the containment coating. Finally, several plants have revised their Emergency Operating Procedures (EOPs

  17. Academic performance and scientific involvement of final year medical students coming from urban and rural backgrounds.

    Science.gov (United States)

    Polasek, Ozren; Kolcic, Ivana

    2006-01-01

    The aim of this study was to evaluate the academic performance and research involvement of students coming from urban and rural backgrounds. To our knowledge, this is the first such study of undergraduate medical students in Croatia. We surveyed the final (sixth) year medical students from Medical School, University of Zagreb, Croatia. Students were surveyed during the academic year 2004/2005, several months prior to their graduation. We analysed students' academic performance (grade point average, which is the most important academic success indicator in Croatia, and the number of failed study years) and research involvement (involvement in research projects and the possible subsequent publication of scientific articles). Additionally, we investigated the extent of extracurricular activities, and students' workplace preferences. Data were analysed using chi2 test, Fisher's exact test, and Kruskal-Wallis test, due to non-normal data distribution. A total of 204 students (out of 240 enrolled, with a response rate of 85%) were surveyed, and divided into three groups: (1) those coming from the highly urbanised background (capital and other highly urbanised areas; n = 100, 49%); (2) mid-urban (towns; n = 75, 37%); and (3) rural and remote backgrounds (n = 29, 14%). There was no indication of gender gap or age difference among the three groups. However, significant differences were recorded in most academic and research indicators. Students from highly urbanised backgrounds reported the best grade point average (p = 0.022). Students from rural and remote backgrounds most commonly reported a study year failure (p = 0.032), with 17 (59%) cases, compared with 32 (32%) and 31 (42%) cases in high and mid-urban ones, respectively. Rural and remote students were also the most likely to experience multiple years failure (p = 0.030), were the least often involved in research projects (p = 0.002), and reported the least interest in career supplemented with scientific research (p

  18. Serum herpes simplex antibodies

    Science.gov (United States)

    ... causes cold sores (oral herpes). HSV-2 causes genital herpes. How the Test is Performed A blood sample ... person has ever been infected with oral or genital herpes . It looks for antibodies to herpes simplex virus ...

  19. FY2014 FES (Fusion Energy Sciences) Theory & Simulation Performance Target, Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Guoyong [Princeton Plasma Physics Lab. (PPPL), Princeton, NJ (United States); Budny, Robert [Princeton Plasma Physics Lab. (PPPL), Princeton, NJ (United States); Gorelenkov, Nikolai [Princeton Plasma Physics Lab. (PPPL), Princeton, NJ (United States); Poli, Francesca [Princeton Plasma Physics Lab. (PPPL), Princeton, NJ (United States); Chen, Yang [Univ. of Colorado, Boulder, CO (United States); McClenaghan, Joseph [Univ. of California, Irvine, CA (United States); Lin, Zhihong [Univ. of California, Irvine, CA (United States); Spong, Don [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bass, Eric [Univ. of California, San Diego, CA (United States); Waltz, Ron [General Atomics, San Diego, CA (United States)

    2014-10-14

    We report here the work done for the FY14 OFES Theory Performance Target as given below: "Understanding alpha particle confinement in ITER, the world's first burning plasma experiment, is a key priority for the fusion program. In FY 2014, determine linear instability trends and thresholds of energetic particle-driven shear Alfven eigenmodes in ITER for a range of parameters and profiles using a set of complementary simulation models (gyrokinetic, hybrid, and gyrofluid). Carry out initial nonlinear simulations to assess the effects of the unstable modes on energetic particle transport". In the past year (FY14), a systematic study of the alpha-driven Alfven modes in ITER has been carried out jointly by researchers from six institutions involving seven codes including the transport simulation code TRANSP (R. Budny and F. Poli, PPPL), three gyrokinetic codes: GEM (Y. Chen, Univ. of Colorado), GTC (J. McClenaghan, Z. Lin, UCI), and GYRO (E. Bass, R. Waltz, UCSD/GA), the hybrid code M3D-K (G.Y. Fu, PPPL), the gyro-fluid code TAEFL (D. Spong, ORNL), and the linear kinetic stability code NOVA-K (N. Gorelenkov, PPPL). A range of ITER parameters and profiles are specified by TRANSP simulation of a hybrid scenario case and a steady-state scenario case. Based on the specified ITER equilibria linear stability calculations are done to determine the stability boundary of alpha-driven high-n TAEs using the five initial value codes (GEM, GTC, GYRO, M3D-K, and TAEFL) and the kinetic stability code (NOVA-K). Both the effects of alpha particles and beam ions have been considered. Finally, the effects of the unstable modes on energetic particle transport have been explored using GEM and M3D-K.

  20. Antibodies immobilized on magnetic particles for radioimmunoassay and immunoradiometric assay of hormones. Final report of a co-ordinated research programme 1991-1995

    International Nuclear Information System (INIS)

    1996-11-01

    The IAEA organized a co-ordinated research programme (CRP) in 1991 for studying the properties of a few most promising magnetizable immunoadsorbents and standardizing some important radioimmunoassay and immunoradiometric assay procedures using them with the ultimate aim of expanding the application of these assays in developing countries using indigenously prepared reagents. Ten laboratories from nine countries of Asia, Latin America and Europe participated in this CRP which was concluded in 1995. Three different magnetizable particles prepared and investigated by the participants, namely magnetite, magnetite coated with silane and magnetite coated with polyacrolein, have emerged suitable for use in radioimmunometric assays from this CRP. Methods have been developed for coupling antibodies to these particles and using the resultant immunoadsorbents for assaying several important hormones and proteins including T 3 , T 4 , fT 3 , fT 4 , reverse T 3 , TSH, thyroglobuling(Tg), Tg-antibodies, HCG, LH, cortisol, FSH and prolacting. All the participating laboratories could develop in house methodology for solid phase assays based on magnetizable particles during the course of the CRP and benefit from the exchange of materials, information and experience amongst them. This report includes detailed results obtained by the participating laboratories as well as a summary and assessment of the achievements of the CRP. It also includes suggestions for areas of investigation for pursuing in the future. Refs, figs, tabs

  1. Final Technical Report: Effects of Impurities on Fuel Cell Performance and Durability

    Energy Technology Data Exchange (ETDEWEB)

    James G. Goodwin, Jr.; Hector Colon-Mercado; Kitiya Hongsirikarn; and Jack Z. Zhang

    2011-11-11

    accessible for hydrogen activation. Of the impurities studied, CO, NH3, perchloroethylene (also known as tetrachloroethylene), tetrahydrofuran, diborane, and metal cations had significant negative effects on the components in a fuel cell. While CO has no effect on the Nafion, it significantly poisons the Pt catalyst by adsorbing and blocking hydrogen activation. The effect can be reversed with time once the flow of CO is stopped. NH3 has no effect on the Pt catalyst at fuel cell conditions; it poisons the proton sites on Nafion (by forming NH4+ cations), decreasing drastically the proton conductivity of Nafion. This poisoning can slowly be reversed once the flow of NH3 is stopped. Perchloroethylene has a major effect on fuel cell performance. Since it has little/no effect on Nafion conductivity, its poisoning effect is on the Pt catalyst. However, this effect takes place primarily for the Pt catalyst at the cathode, since the presence of oxygen is very important for this poisoning effect. Tetrahydrofuran was shown not to impact Nafion conductivity; however, it does affect fuel cell performance. Therefore, its primary effect is on the Pt catalyst. The effect of THF on fuel cell performance is reversible. Diborane also can significant affect fuel cell performance. This effect is reversible once diborane is removed from the inlet streams. H2O2 is not an impurity usually present in the hydrogen or oxygen streams to a fuel cell. However, it is generated during fuel cell operation. The presence of Fe cations in the Nafion due to system corrosion and/or arising from MEA production act to catalyze the severe degradation of the Nafion by H2O2. Finally, the presence of metal cation impurities (Na+, Ca 2+, Fe3+) in Nafion from MEA preparation or from corrosion significantly impacts its proton conductivity due to replacement of proton sites. This effect is not reversible. Hydrocarbons, such as ethylene, might be expected to affect Pt or Nafion but do not at a typical fuel cell

  2. Performance Evaluation of the VIDAS® Measles IgG Assay and Its Diagnostic Value for Measuring IgG Antibody Avidity in Measles Virus Infection

    Science.gov (United States)

    Dina, Julia; Creveuil, Christian; Gouarin, Stephanie; Viron, Florent; Hebert, Amelie; Freymuth, Francois; Vabret, Astrid

    2016-01-01

    The objective of this study is primarily to compare the performance of the VIDAS® Measles immunoglobulin (Ig)G assay to that of two other serological assays using an immunoassay technique, Enzygnost® Anti-measles Virus/IgG (Siemens) and Measles IgG CAPTURE EIA® (Microimmune). The sensitivity and the agreement of the VIDAS® Measles IgG assay compared to the Enzygnost® Anti-measles Virus/IgG assay and the Measles IgG CAPTURE EIA® assay are 100%, 97.2% and 99.0%, 98.4%, respectively. The very low number of negative sera for IgG antibodies does not allow calculation of specificity. As a secondary objective, we have evaluated the ability of the VIDAS® Measles IgG assay to measure anti-measles virus IgG antibody avidity with the help of the VIDAS® CMV IgG Avidity reagent, using 76 sera from subjects with measles and 238 other sera. Different groups of populations were analyzed. In the primary infection measles group, the mean IgG avidity index was 0.16 (range of 0.07 to 0.93) compared to 0.79 (range of 0.25 to 1) in the serum group positive for IgG antibodies and negative for IgM. These data allow to define a weak anti-measles virus IgG antibody avidity as an avidity index (AI) 0.6. The VIDAS® Measles IgG assay has a performance equivalent to that of other available products. Its use, individual and quick, is well adapted to testing for anti-measles immunity in exposed subjects. PMID:27556477

  3. Self-management support by final year nursing students: A correlational study of performance and person-related associated factors.

    Science.gov (United States)

    Duprez, Veerle; Beeckman, Dimitri; Verhaeghe, Sofie; Van Hecke, Ann

    2017-09-01

    Chronic conditions put a heavy burden on healthcare in every country. Supporting persons with a chronic illness to take an active role in the management of their condition is a core component in the Chronic Care Model. It implies confidence and good skills from professionals. To date, there is no evidence on final year nursing students' performance in supporting patients' self-management, nor on factors associated with this performance. To explore self-reported performance of supporting patients' self-management by final year nursing students, and person-related factors associated with this performance. A correlational multi-centre study of final year nursing students (N=256) from eight nursing schools. Students were recruited from a convenience sample of eight nursing schools. All final year students were invited to participate. Data were collected between January 2015 and May 2016 using self-administered validated questionnaires. Theoretical behavioural frameworks were used to select hypothesized associated factors for self-management support: self-efficacy to perform self-management support and socio-structural factors (Social Cognitive Theory); needs for autonomy, competence and relatedness, and patient-invested contingent self-esteem (Self-Determination Theory); and attitudes towards supporting patients' self-management (Theory of Planned Behaviour). Final year nursing students (N=256) reported an overall low level of performance in delivering self-management support during internship. Students lacked mainly competencies in collaborative goal setting and shared decision making. Students reported a significant gap between their confidence and their actual performance in self-management support (pLearning opportunities can be introduced in classroom activities and on internship. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Emotional intelligence and academic performance in first and final year medical students: a cross-sectional study.

    Science.gov (United States)

    Chew, Boon How; Zain, Azhar Md; Hassan, Faezah

    2013-03-27

    Research on emotional intelligence (EI) suggests that it is associated with more pro-social behavior, better academic performance and improved empathy towards patients. In medical education and clinical practice, EI has been related to higher academic achievement and improved doctor-patient relationships. This study examined the effect of EI on academic performance in first- and final-year medical students in Malaysia. This was a cross-sectional study using an objectively-scored measure of EI, the Mayer-Salovey-Caruso Emotional Intelligence Test (MSCEIT). Academic performance of medical school students was measured using continuous assessment (CA) and final examination (FE) results. The first- and final-year students were invited to participate during their second semester. Students answered a paper-based demographic questionnaire and completed the online MSCEIT on their own. Relationships between the total MSCEIT score to academic performance were examined using multivariate analyses. A total of 163 (84 year one and 79 year five) medical students participated (response rate of 66.0%). The gender and ethnic distribution were representative of the student population. The total EI score was a predictor of good overall CA (OR 1.01), a negative predictor of poor result in overall CA (OR 0.97), a predictor of the good overall FE result (OR 1.07) and was significantly related to the final-year FE marks (adjusted R(2) = 0.43). Medical students who were more emotionally intelligent performed better in both the continuous assessments and the final professional examination. Therefore, it is possible that emotional skill development may enhance medical students' academic performance.

  5. Antiprothrombin Antibodies

    Directory of Open Access Journals (Sweden)

    Polona Žigon

    2015-05-01

    Full Text Available In patients with the antiphospholipid syndrome (APS, the presence of a group of pathogenic autoantibodies called antiphospholipid antibodies causes thrombosis and pregnancy complications. The most frequent antigenic target of antiphospholipid antibodies are phospholipid bound β2-glycoprotein 1 (β2GPI and prothrombin. The international classification criteria for APS connect the occurrence of thrombosis and/or obstetric complications together with the persistence of lupus anticoagulant, anti-cardiolipin antibodies (aCL and antibodies against β2GPI (anti-β2GPI into APS. Current trends for the diagnostic evaluation of APS patients propose determination of multiple antiphospholipid antibodies, among them also anti-prothrombin antibodies, to gain a common score which estimates the risk for thrombosis in APS patients. Antiprothrombin antibodies are common in APS patients and are sometimes the only antiphospholipid antibodies being elevated. Methods for their determination differ and have not yet been standardized. Many novel studies confirmed method using phosphatidylserine/prothrombin (aPS/PT ELISA as an antigen on solid phase encompass higher diagnostic accuracy compared to method using prothrombin alone (aPT ELISA. Our research group developed an in-house aPS/PT ELISA with increased analytical sensitivity which enables the determination of all clinically relevant antiprothrombin antibodies. aPS/PT exhibited the highest percentage of lupus anticoagulant activity compared to aCL and anti-β2GPI. aPS/PT antibodies measured with the in-house method associated with venous thrombosis and presented the strongest independent risk factor for the presence of obstetric complications among all tested antiphospholipid antibodies

  6. Self-management support by final year nursing students : a correlational study of performance and person-related associated factors

    OpenAIRE

    Duprez, Veerle; Beeckman, Dimitri; Verhaeghe, Sofie; Van Hecke, Ann

    2017-01-01

    Background Chronic conditions put a heavy burden on healthcare in every country. Supporting persons with a chronic illness to take an active role in the management of their condition is a core component in the Chronic Care Model. It implies confidence and good skills from professionals. To date, there is no evidence on final year nursing students’ performance in supporting patients’ self-management, nor on factors associated with this performance. Objectives To explore self-reporte...

  7. The Indiana Deafblind Services Project: Services for Children with Deafblindness Program. Final Performance Report.

    Science.gov (United States)

    Indiana State Univ., Terre Haute. Blumberg Center for Interdisciplinary Studies in Special Education.

    This final report describes activities and accomplishments of the Indiana Deaf-Blind Services Project, a 4-year federally funded project to enhance and further develop coordinated direct services to children and youth, birth through 2 and ages 18 through 21. The project also was designed to provide technical assistance to public and private…

  8. Do Work Placements Improve Final Year Academic Performance or Do High-Calibre Students Choose to Do Work Placements?

    Science.gov (United States)

    Jones, C. M.; Green, J. P.; Higson, H. E.

    2017-01-01

    This study investigates whether the completion of an optional sandwich work placement enhances student performance in final year examinations. Using Propensity Score Matching, our analysis departs from the literature by controlling for self-selection. Previous studies may have overestimated the impact of sandwich work placements on performance…

  9. Lewistown City Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    Science.gov (United States)

    Stead, Sharon

    This final performance report for the Lewistown City Library L.E.A.R.N. (Let Every Adult Read Now!) literacy project begins with a section that provides quantitative data. The next section compares actual accomplishments to the following project goals for 1992-93: to serve a minimum of 25 adult literacy students within an 18-month period and to…

  10. Safety and operational performance evaluation of four types of exit ramps on Florida's freeways (final report).

    Science.gov (United States)

    2010-12-01

    This project mainly focuses on exit ramp performance analysis of safety and operations. In addition, issues of advance guide sign for exit ramp are also mentioned. : Safety analysis evaluates safety performances of different exit ramps used in Florid...

  11. Knowledge-Based Parallel Performance Technology for Scientific Application Competitiveness Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Malony, Allen D; Shende, Sameer

    2011-08-15

    The primary goal of the University of Oregon's DOE "œcompetitiveness" project was to create performance technology that embodies and supports knowledge of performance data, analysis, and diagnosis in parallel performance problem solving. The target of our development activities was the TAU Performance System and the technology accomplishments reported in this and prior reports have all been incorporated in the TAU open software distribution. In addition, the project has been committed to maintaining strong interactions with the DOE SciDAC Performance Engineering Research Institute (PERI) and Center for Technology for Advanced Scientific Component Software (TASCS). This collaboration has proved valuable for translation of our knowledge-based performance techniques to parallel application development and performance engineering practice. Our outreach has also extended to the DOE Advanced CompuTational Software (ACTS) collection and project. Throughout the project we have participated in the PERI and TASCS meetings, as well as the ACTS annual workshops.

  12. TGLF Recalibration for ITER Standard Case Parameters FY2015: Theory and Simulation Performance Target Final Report

    International Nuclear Information System (INIS)

    Candy, J.

    2015-01-01

    flows that fit the GYRO simulations, the parameters of the model had to be tuned to each case. A physics basis for the zonal flow model was lacking. Electron energy transport at short wavelength: A secondary issue - the high-k electron energy flux - was initially assumed to be independent of the zonal flow effect. However, detailed studies of the fluctuation spectra from recent multiscale (electron and ion scale) GYRO simulations provided a critical new insight into the role of zonal flows. The multiscale simulations suggested that advection by the zonal flows strongly suppressed electron-scale turbulence. Radial shear of the zonal ExB fluctuation could not compete with the large electron-scale linear growth rate, but the k x -mixing rate of the ExB advection could. This insight led to a preliminary new model for the way zonal flows saturate both electron- and ion-scale turbulence. It was also discovered that the strength of the zonal ExB velocity could be computed from the linear growth rate spectrum. The new saturation model (SAT1), which replaces the original model (SAT0), was fit to the multiscale GYRO simulations as well as the ion-scale GYRO simulations used to calibrate the original SAT0 model. Thus, SAT1 captures the physics of both multiscale electron transport and zonal-flow stabilization. In future work, the SAT1 model will require significant further testing and (expensive) calibration with nonlinear multiscale gyrokinetic simulations over a wider variety of plasma conditions - certainly more than the small set of scans about a single C-Mod L-mode discharge. We believe the SAT1 model holds great promise as a physics-based model of the multiscale turbulent transport in fusion devices. Correction to ITER performance predictions: Finally, the impact of the SAT1model on the ITER hybrid case is mixed. Without the electron-scale contribution to the fluxes, the Dimits shift makes a significant improvement in the predicted fusion power as originally posited. Alas

  13. TGLF Recalibration for ITER Standard Case Parameters FY2015: Theory and Simulation Performance Target Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Candy, J. [General Atomics, San Diego, CA (United States)

    2015-12-01

    flows that fit the GYRO simulations, the parameters of the model had to be tuned to each case. A physics basis for the zonal flow model was lacking. Electron energy transport at short wavelength: A secondary issue – the high-k electron energy flux – was initially assumed to be independent of the zonal flow effect. However, detailed studies of the fluctuation spectra from recent multiscale (electron and ion scale) GYRO simulations provided a critical new insight into the role of zonal flows. The multiscale simulations suggested that advection by the zonal flows strongly suppressed electron-scale turbulence. Radial shear of the zonal E×B fluctuation could not compete with the large electron-scale linear growth rate, but the kx-mixing rate of the E×B advection could. This insight led to a preliminary new model for the way zonal flows saturate both electron- and ion-scale turbulence. It was also discovered that the strength of the zonal E×B velocity could be computed from the linear growth rate spectrum. The new saturation model (SAT1), which replaces the original model (SAT0), was fit to the multiscale GYRO simulations as well as the ion-scale GYRO simulations used to calibrate the original SAT0 model. Thus, SAT1 captures the physics of both multiscale electron transport and zonal-flow stabilization. In future work, the SAT1 model will require significant further testing and (expensive) calibration with nonlinear multiscale gyrokinetic simulations over a wider variety of plasma conditions – certainly more than the small set of scans about a single C-Mod L-mode discharge. We believe the SAT1 model holds great promise as a physics-based model of the multiscale turbulent transport in fusion devices. Correction to ITER performance predictions: Finally, the impact of the SAT1model on the ITER hybrid case is mixed. Without the electron-scale contribution to the fluxes, the Dimits shift makes a significant improvement in the predicted fusion power as

  14. Final Report - Effects of Impurities on Fuel Cell Performance and Durability

    Energy Technology Data Exchange (ETDEWEB)

    Trent Molter

    2012-08-18

    This program is focused on the experimental determination of the effects of key hydrogen side impurities on the performance of PEM fuel cells. Experimental data has been leveraged to create mathematical models that predict the performance of PEM fuel cells that are exposed to specific impurity streams. These models are validated through laboratory experimentation and utilized to develop novel technologies for mitigating the effects of contamination on fuel cell performance. Results are publicly disseminated through papers, conference presentations, and other means.

  15. Diagnostic performance of ELISA, IFAT and Western blot for the detection of anti-Leishmania infantum antibodies in cats using a Bayesian analysis without a gold standard.

    Science.gov (United States)

    Persichetti, Maria Flaminia; Solano-Gallego, Laia; Vullo, Angela; Masucci, Marisa; Marty, Pierre; Delaunay, Pascal; Vitale, Fabrizio; Pennisi, Maria Grazia

    2017-03-13

    Anti-Leishmania antibodies are increasingly investigated in cats for epidemiological studies or for the diagnosis of clinical feline leishmaniosis. The immunofluorescent antibody test (IFAT), the enzyme-linked immunosorbent assay (ELISA) and western blot (WB) are the serological tests more frequently used. The aim of the present study was to assess diagnostic performance of IFAT, ELISA and WB to detect anti-L. infantum antibodies in feline serum samples obtained from endemic (n = 76) and non-endemic (n = 64) areas and from cats affected by feline leishmaniosis (n = 21) by a Bayesian approach without a gold standard. Cut-offs were set at 80 titre for IFAT and 40 ELISA units for ELISA. WB was considered positive in presence of at least a 18 KDa band. Statistical analysis was performed through a written routine with MATLAB software in the Bayesian framework. The latent data and observations from the joint posterior were simulated in the Bayesian approach by an iterative Markov Chain Monte Carlo technique using the Gibbs sampler for estimating sensitivity and specificity of the three tests. The median seroprevalence in the sample used for evaluating the performance of tests was estimated at 0.27 [credible interval (CI) = 0.20-0.34]. The median sensitivity of the three different methods was 0.97 (CI: 0.86-1.00), 0.75 (CI: 0.61-0.87) and 0.70 (CI: 0.56-0.83) for WB, IFAT and ELISA, respectively. Median specificity reached 0.99 (CI: 0.96-1.00) with WB, 0.97 (CI: 0.93-0.99) with IFAT and 0.98 (CI: 0.94-1.00) with ELISA. IFAT was more sensitive than ELISA (75 vs 70%) for the detection of subclinical infection while ELISA was better for diagnosing clinical leishmaniosis when compared with IFAT (98 vs 97%). The overall performance of all serological techniques was good and the most accurate test for anti-Leishmania antibody detection in feline serum samples was WB.

  16. National Aeronautics and Space Administration FY 02 Revised Final Annual Performance Plan

    National Research Council Canada - National Science Library

    2002-01-01

    ...) Improve citizen confidence in government performance. GPRA directs Executive Branch agencies to develop a customer-focused strategic plan that aligns activities with concrete missions and goals...

  17. UCSD Performance in the Edge Plasma Simulation (EPSI) Project. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Tynan, George Robert [Univ. of California, San Diego, CA (United States). Center for Energy Research

    2017-12-12

    This report contains a final report on the activities of UC San Diego PI G.R. Tynan and his collaborators as part of the EPSI Project, that was led by Dr. C.S. Chang, from PPPL. As a part of our work, we carried out several experiments on the ALCATOR C-­MOD tokamak device, aimed at unraveling the “trigger” or cause of the spontaneous transition from low-­mode confinement (L-­mode) to high confinement (H-­mode) that is universally observed in tokamak devices, and is planned for use in ITER.

  18. Performance Engineering Research Institute SciDAC-2 Enabling Technologies Institute Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Hall, Mary [University of Utah

    2014-09-19

    Enhancing the performance of SciDAC applications on petascale systems has high priority within DOE SC. As we look to the future, achieving expected levels of performance on high-end com-puting (HEC) systems is growing ever more challenging due to enormous scale, increasing archi-tectural complexity, and increasing application complexity. To address these challenges, PERI has implemented a unified, tripartite research plan encompassing: (1) performance modeling and prediction; (2) automatic performance tuning; and (3) performance engineering of high profile applications. The PERI performance modeling and prediction activity is developing and refining performance models, significantly reducing the cost of collecting the data upon which the models are based, and increasing model fidelity, speed and generality. Our primary research activity is automatic tuning (autotuning) of scientific software. This activity is spurred by the strong user preference for automatic tools and is based on previous successful activities such as ATLAS, which has automatically tuned components of the LAPACK linear algebra library, and other re-cent work on autotuning domain-specific libraries. Our third major component is application en-gagement, to which we are devoting approximately 30% of our effort to work directly with Sci-DAC-2 applications. This last activity not only helps DOE scientists meet their near-term per-formance goals, but also helps keep PERI research focused on the real challenges facing DOE computational scientists as they enter the Petascale Era.

  19. Automobile driver on-road performance test. Volume 1, final report

    Science.gov (United States)

    1981-09-30

    The Automobile Driver On-Road Performance Test (ADOPT) was developed during a three-phase project. In Phase 1, 51 candidate behaviors were identified and selected with the help of experts in the fields of traffic safety, measurement of driver perform...

  20. Designing Scholarships to Improve College Success: Final Report on the Performance-Based Scholarship Demonstration

    Science.gov (United States)

    Mayer, Alexander K.; Patel, Reshma; Rudd, Timothy; Ratledge, Alyssa

    2015-01-01

    Performance-based scholarships have two main goals: (1) to give students more money for college; and (2) to provide incentives for academic progress. MDRC launched the Performance-Based Scholarship (PBS) Demonstration in 2008 to evaluate the effectiveness of these scholarships in a diverse set of states, institutions, and low-income student…

  1. High performance low cost interconnections for flip chip attachment with electrically conductive adhesive. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-05-01

    This final report is a compilation of final reports from each of the groups participating in the program. The main three groups involved in this effort are the Thomas J. Watson Research Center of IBM Corporation in Yorktown Heights, New York, Assembly Process Design of IBM Corporation in Endicott, New York, and SMT Laboratory of Universal Instruments Corporation in Binghamton, New York. The group at the research center focused on the conductive adhesive materials development and characterization. The group in process development focused on processing of the Polymer-Metal-Solvent Paste (PMSP) to form conductive adhesive bumps, formation of the Polymer-Metal Composite (PMC) on semiconductor devices and study of the bonding process to circuitized organic carriers, and the long term durability and reliability of joints formed using the process. The group at Universal Instruments focused on development of an equipment set and bonding parameters for the equipment to produce bond assembly tooling. Reports of each of these individual groups are presented here reviewing their technical efforts and achievements.

  2. Utility and performance relative to consumer product energy efficiency standards. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    Coggins, J.L.

    1979-12-14

    An investigation of the relative utility and performance of nine major household consumer products covered by the Energy Policy and Conservation Act is summarized. The objective was to define the terms utility and performance, to recommend methods for quantifying these two concepts, and to recommend an approach for dealing with utility and performance issues in the energy efficiency standards program. The definitions developed are: performance of a consumer product is the objective measure of how well, with the expected level of consumer input (following the manufacturer's instructions for installation and operation), the product does its intended job; and utility of a consumer product is a subjective measure, based on the consumer's perception, of the capability of the product to satisfy human needs. Quantification is based on test procedures and consumer survey methods which are largely already in use by industry. Utility and performance issues are important in product classification for prescribing energy efficiency standards. The recommended approach to utility and performance issues and classification is: prior to setting standards, evaluate utility and performance issues in the most quantitative way allowed by resources and schedules in order to develop classification guidelines. This approach requires no changes in existing Department of Energy test procedures.

  3. Performance characteristics of a combined hepatitis C virus core antigen and anti–hepatitis C virus antibody test in different patient groups

    Directory of Open Access Journals (Sweden)

    Jeng-Fu Yang

    2011-07-01

    Full Text Available We evaluated the performance of a hepatitis C virus (HCV antigen/antibody combination test [Murex HCV Antigen/Antibody Combination Test (Murex Ag/Ab test] by comparing it with the current third-generation HCV antibody enzyme immunoassay (anti-HCV. A total of 403 serum samples were consecutively collected from four patient groups: healthy controls (n=100; HCV-infected patients (HCV group, n=102; Human immunodeficiency virus (HIV/HCV-infected patients (HIV/HCV group, n=100; and patients with uremia (uremia group, n=101. Performances were evaluated for the Murex Ag/Ab, anti-HCV, and HCV RNA in the HIV/HCV and uremia patient groups. In the HCV group, all 102 samples showed concordant positive and negative results for anti-HCV, Murex Ag/Ab, and HCV RNA tests. In the HIV/HCV group, all 100 samples were positive for both anti-HCV and Murex Ag/Ab tests, whereas 88 patients (88% were HCV RNA positive. In the uremia group, 14 (69.0% of the 23 anti-HCV-positive patients were HCV RNA positive, whereas 14 (77.8% of the 18 Murex Ag/Ab–positive patients were HCV RNA positive. None of anti-HCV-negative or Murex Ag/Ab–negative patients were HCV RNA positive. Based on the HCV RNA assay, the sensitivities for both anti-HCV and Murex Ag/Ab assays were 100%, whereas the specificities of these two assays were 89.7% and 95.4%, respectively. With good sensitivity and specificity, the Murex Ag/Ab assay could be a useful alternative diagnostic tool, especially in immunocompromised populations, such as patients with uremia or those infected with HIV.

  4. Growth Performance, Carcass Characteristics, Antibody Titer and Blood Parameters in Broiler Chickens Fed Dietary Myrtle (Myrtus communis Essential Oil as an Alternative to Antibiotic Growth Promoter

    Directory of Open Access Journals (Sweden)

    Mahmoodi Bardzardi M

    2014-03-01

    Full Text Available This experiment was conducted to determine the effects of Myrtle Essential Oil (MEO on growth performance, carcass characteristics, antibody titer and blood parameters of broiler chickens. A total of 200 Ross 308 broiler chickens were allocated to five dietary treatments with four replicates of 10 birds each. Dietary treatments were prepared by formulating a corn-soybean meal-based diet free of antibiotics (Control and supplementing the basal diet with three levels of MEO at 100, 200, 300 mg/Kg or antibiotic Flavophospholipol (FPL at 600 mg/Kg. The results showed that diets supplemented with MEO and FPL increased the feed intake, body weight gain and improved the feed conversion ratio compared to the control treatment (P. The relative carcass weight was significantly increased, whereas the weight of gastrointestinal tract and liver were decreased in broilers fed MEO (P. Supplementing the basal diet with MEO increased the antibody titers against Avian Influenza Virus (AIV and Newcastle disease Virus (NDV, although supplementing diet with 200 mg/Kg of MEO was more effective (P. Broilers fed MEO diets especially at the level of 300 mg/Kg had a lower white blood cells count and heterophil, heterophil to lymphocyte ratio, mean corpuscular volume and mean corpuscular hemoglobin, but a higher lymphocyte and red blood cells count (P. In conclusion, data showed that diet supplemented with MEO improved the growth performance and increased antibody titers against AIV and NDV, especially at the level of 200 mg/Kg, in broiler chickens and could be an adequate alternative to antibiotics.

  5. Bifunctional antibodies for radioimmunotherapy.

    Science.gov (United States)

    Chatal, J F; Faivre-Chauvet, A; Bardies, M; Peltier, P; Gautherot, E; Barbet, J

    1995-04-01

    In two-step targeting technique using bifunctional antibodies, a nonradiolabeled immunoconjugate with slow uptake kinetics (several days) is initially injected, followed by a small radiolabeled hapten with fast kinetics (several hours) that binds to the bispecific immunoconjugate already taken up by the tumor target. In patients with colorectal or medullary thyroid cancer, clinical studies performed with an anti-CEA/anti-DTPA-indium bifunctional antibody and an indium-111-labeled di-DTPA-TL bivalent hapten showed that tumor uptake was not modified compared to results for F(ab')2 fragments of the same anti-CEA antibody directly labeled with indium-111, whereas the radioactivity of normal tissues was significantly reduced (3- to 6-fold). The fast tumor uptake kinetics (several hours) and high or very high tumor-to-normal tissue ratios obtained with the bifunctional antibody technique are favorable parameters for efficient radioimmunotherapy.

  6. A-jacks and Aquawrap installations in Utah : scour revetment performance evaluation, final report, December 2009.

    Science.gov (United States)

    2009-12-01

    This is a performance evaluation report for A-Jacks, an articulated concrete block designed to protect bridge elements exposed to the river scouring forces, and for Aquawrap, a glass fiber reinforced polymer designed to protect and strengthen bridge ...

  7. Improved prediction models for PCC pavement performance-related specifications, volume I : final report.

    Science.gov (United States)

    2000-12-01

    The current performance-related specifications (PRS) methodology has been under development by the Federal : Highway Administration (FHWA) for several years and has now reached a level at which it can be implemented by : State highway agencies. PRS f...

  8. National Aeronautics and Space Administration FY 02 Revised Final Annual Performance Plan

    Science.gov (United States)

    2002-01-01

    The Government Performance and Results Act (GPRA) was passed by Congress and signed by the President in 1993. GPRA was enacted to improve the efficiency of all Federal agencies, with the following specific goals: (1) Improve Federal program management, effectiveness, and public accountability; (2) Improve Congressional decision making on where to commit the Nation's financial and human resources; and (3) Improve citizen confidence in government performance. GPRA directs Executive Branch agencies to develop a customer-focused strategic plan that aligns activities with concrete missions and goals. The Act directs agencies to manage and measure results to justify Congressional appropriations and authorizations. The Report Consolidation Act of 2000 directs agencies to provide a report on the degree of success in achieving the goals and performance measures defined in the strategic and performance plans one hundred and fifty days after the completion of the fiscal year.

  9. Performance measures to characterize directional corridor travel time delay based on probe vehicle data : final report.

    Science.gov (United States)

    2015-10-01

    Anonymous probe vehicle data are currently being collected on roadways throughout the United States. These data are being incorporated into local and statewide mobility reports to measure the performance of freeways and arterial systems. Predefined s...

  10. Workshop on programming languages for high performance computing (HPCWPL): final report.

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, Richard C.

    2007-05-01

    This report summarizes the deliberations and conclusions of the Workshop on Programming Languages for High Performance Computing (HPCWPL) held at the Sandia CSRI facility in Albuquerque, NM on December 12-13, 2006.

  11. Comparison of the performance of five different immunoassays to detect specific antibodies against emerging atypical bovine pestivirus

    DEFF Research Database (Denmark)

    Larska, Magdalena; Polak, Mirosław P.; Liu, Lihong

    2013-01-01

    Bovine pestiviruses represent a considerably variable group. In addition to the two accepted species BVDV-1 and BVDV-2, a number of atypical bovine pestiviruses have been detected both in foetal calf sera and in field samples. The sera collected during the initial six weeks of experimental...... infection of calves with atypical pestivirus, BVDV-1 and a combination of both viruses have been examined by routine and new diagnostic tests to validate their robustness and sensitivity. As expected, virus neutralization tests using homologous virus were able to differentiate the two groups infected...... by BVDV-1 or atypical pestivirus, whereas the animals inoculated with a mixture of these two viruses had a reaction pattern very similar to the homologous virus alone. It was found that immunoassays using whole virus and polyclonal antibodies are the most robust, but all tests examined were able to detect...

  12. Quality Assurance Strategy for Existing Homes. Final Quality Management Primer for High Performing Homes

    Energy Technology Data Exchange (ETDEWEB)

    Del Bianco, M. [NAHB Research Center Industry Partnership, Upper Marlboro, MD (United States); Taggart, J. [NAHB Research Center Industry Partnership, Upper Marlboro, MD (United States); Sikora, J. [NAHB Research Center Industry Partnership, Upper Marlboro, MD (United States); Wood, A. [NAHB Research Center Industry Partnership, Upper Marlboro, MD (United States)

    2012-12-01

    This guide is designed to help Building America (BA) teams understand quality management and its role in transitioning from conventional to high performance home building and remodeling. It explains what quality means, the value of quality management systems, the unique need for QMS when building high performing homes, and the first steps to a implementing a comprehensive QMS. This document provides a framework and context for BA teams when they encounter builders and remodelers.

  13. Quality Assurance Strategy for Existing Homes: Final Quality Management Primer for High Performing Homes

    Energy Technology Data Exchange (ETDEWEB)

    Del Bianco, M.; Taggart, J.; Sikora, J.; Wood, A.

    2012-12-01

    This guide is designed to help Building America (BA) Teams understand quality management and its role in transitioning from conventional to high performance home building and remodeling. It explains what quality means, the value of quality management systems, the unique need for QMS when building high performing homes, and the first steps to a implementing a comprehensive QMS. This document provides a framework and context for BA teams when they encounter builders and remodelers.

  14. Performance Engineering Research Institute SciDAC-2 Enabling Technologies Institute Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Lucas, Robert

    2013-04-20

    Enhancing the performance of SciDAC applications on petascale systems had high priority within DOE SC at the start of the second phase of the SciDAC program, SciDAC-2, as it continues to do so today. Achieving expected levels of performance on high-end computing (HEC) systems is growing ever more challenging due to enormous scale, increasing architectural complexity, and increasing application complexity. To address these challenges, the University of Southern California?s Information Sciences Institute organized the Performance Engineering Research Institute (PERI). PERI implemented a unified, tripartite research plan encompassing: (1) performance modeling and prediction; (2) automatic performance tuning; and (3) performance engineering of high profile applications. Within PERI, USC?s primary research activity was automatic tuning (autotuning) of scientific software. This activity was spurred by the strong user preference for automatic tools and was based on previous successful activities such as ATLAS, which automatically tuned components of the LAPACK linear algebra library, and other recent work on autotuning domain-specific libraries. Our other major component was application engagement, to which we devoted approximately 30% of our effort to work directly with SciDAC-2 applications. This report is a summary of the overall results of the USC PERI effort.

  15. Evaluation of the Field Performance of Residential Fuel Cells: Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Torrero, E.; McClelland, R.

    2004-05-01

    Distributed generation has attracted significant interest from rural electric cooperatives and their customers. Cooperatives have a particular nexus because of inherently low customer density, growth patterns at the end of long lines, and an influx of customers and high-tech industries seeking to diversify out of urban environments. Fuel cells are considered a particularly interesting DG candidate for these cooperatives because of their power quality, efficiency, and environmental benefits. The National Rural Electric Cooperative Association Cooperative Research Network residential fuel cell program demonstrated RFC power plants and assessed related technical and application issues. This final subcontract report is an assessment of the program's results. This 3-year program leveraged Department of Energy (DOE) and National Renewable Energy Laboratory (NREL) funding.

  16. Antimisting kerosene: evaluation of low-temperature performance. Final report, March 1983-December 1984

    Energy Technology Data Exchange (ETDEWEB)

    Parikh, P.; Yavrouian, A.; Petersen, R.; Sarohia, V.

    1986-08-01

    The low-temperature and freezing behavior of Jet A and AMK fuels was investigated in a 50-gallon capacity wing-tank simulator. The fuel in the rectangular simulator was chilled from the top and bottom surfaces while the sides were thermally insulated. The evolution of a vertical temperature profile in the simulator was studied for Jet A and AMK fuels under nearly identical bottom-wall temperature histories. A small but noticeable difference between the bulk-fuel thermal response of Jet A and AMK was observed with a slower response for AMK. Holdup measurements for AMK were slightly lower than those for Jet A. Rocking of the simulated wing tank did not significantly alter the cool down and freezing behavior. The performance of two boost pumps: one for the DC-10 and the other for the Cessna 441 aircraft, was evaluated with AMK fuel. For both pumps the performance deteriorated when switching from Jet A to AMK. This performance deterioration was far more dramatic for the Cessna 441 boost pump than for the DC-10 boost pump. At low temperature (-30 C) the performance of the DC-10 pump actually improved compared to ambient temperature (20 C) performance. It deteriorated, however, for the Cessna 441 pump.

  17. Lithium/Manganese Dioxide (Li/MnO(2)) Battery Performance Evaluation: Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Ingersoll, D.; Clark, N.H.

    1999-04-01

    In February 1997, under the auspices of the Product Realization Program, an initiative to develop performance models for lithium/manganese dioxide-based batteries began. As a part of this initiative, the performance characteristics of the cells under a variety of conditions were determined, both for model development and for model validation. As a direct result of this work, it became apparent that possible Defense Program (DP) uses for batteries based on this cell chemistry existed. A larger effort aimed at mapping the performance envelope of this chemistry was initiated in order to assess the practicality of this cell chemistry, not only for DP applications, but also for other uses. The work performed included an evaluation of the cell performance as a function of a number of variables, including cell size, manufacturer, current, pulse loads, constant current loads, safety, etc. In addition, the development of new evaluation techniques that would apply to any battery system, such as those related to reliability assessments began. This report describes the results of these evaluations.

  18. [Electronic and structural properties of individual nanometer-size supported metallic clusters]. Final performance report

    Energy Technology Data Exchange (ETDEWEB)

    Reifenberger, R.

    1993-09-01

    This report summarizes the work performed under contract DOE-FCO2-84ER45162. During the past ten years, our study of electron emission from laser-illuminated field emission tips has taken on a broader scope by addressing problems of direct interest to those concerned with the unique physical and chemical properties of nanometer-size clusters. The work performed has demonstrated that much needed data can be obtained on individual nanometer-size clusters supported on a wide-variety of different substrates. The work was performed in collaboration with R.P. Andres in the School of Chemical Engineering at Purdue University. The Multiple Expansion Cluster Source developed by Andres and his students was essential for producing the nanometer-size clusters studied. The following report features a discussion of these results. This report provides a motivation for studying the properties of nanometer-size clusters and summarizes the results obtained.

  19. Effects of coal-derived trace species on performance of molten carbonate fuel cells. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1992-05-01

    The Carbonate Fuel Cell is a very promising option for highly efficient generation of electricity from many fuels. If coal-gas is to be used, the interactions of coal-derived impurities on various fuel cell components need to be understood. Thus the effects on Carbonate Fuel Cell performance due to ten different coal-derived contaminants viz., NH{sub 3}, H{sub 2}S, HC{ell}, H{sub 2}Se, AsH{sub 3}, Zn, Pb, Cd, Sn, and Hg, have been studied at Energy Research Corporation. Both experimental and theoretical evaluations were performed, which have led to mechanistic insights and initial estimation of qualitative tolerance levels for each species individually and in combination with other species. The focus of this study was to investigate possible coal-gas contaminant effects on the anode side of the Carbonate Fuel Cell, using both out-of-cell thermogravimetric analysis by isothermal TGA, and fuel cell testing in bench-scale cells. Separate experiments detailing performance decay in these cells with high levels of ammonia contamination (1 vol %) and with trace levels of Cd, Hg, and Sn, have indicated that, on the whole, these elements do not affect carbonate fuel cell performance. However, some performance decay may result when a number of the other six species are present, singly or simultaneously, as contaminants in fuel gas. In all cases, tolerance levels have been estimated for each of the 10 species and preliminary models have been developed for six of them. At this stage the models are limited to isothermal, benchscale (300 cm{sup 2} size) single cells. The information obtained is expected to assist in the development of coal-gas cleanup systems, while the contaminant performance effects data will provide useful basic information for modeling fuel cell endurance in conjunction with integrated gasifier/fuel-cell systems (IGFC).

  20. Performance Evaluation of Multivariate Analysis Methods on the $Z \\gamma$ Final State

    CERN Document Server

    Amos, Kieran Robert

    2017-01-01

    The performance of various machine learning algorithms are evaluated for their separation power of the $Z\\gamma$ Electroweak process (with $Z\\rightarrow\\ell\\ell$ and $\\ell=e,\\mu$) against the various backgrounds that populate the selection.\\\\ The Boosted Decision Tree method is found to deliver the best performance and is compared to that of neural net analysis and previously used methods using $36.1\\, \\text{fb}^{-1}$ of data obtained at $\\sqrt{s}=13\\, \\text{TeV}$ from the ATLAS detector in 2015 and 2016.

  1. Articulated, Performance-Based Instruction Objectives Guide for Automotive Mechanics. Final Document. Revised.

    Science.gov (United States)

    Henderson, William Edward, Jr.

    Developed during a project designed to provide continuous, performance-based vocational training at the secondary and postsecondary levels, this instructional guide is intended to help teachers implement a laterally and vertically articulated secondary level automotive mechanics program. Introductory materials include descriptions of Automotive…

  2. Complex Multi-Chamber Airbag Performance Simulation Final Report CRADA No. TSB-961-94

    Energy Technology Data Exchange (ETDEWEB)

    Kay, Gregory [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kithil, Philip [Advanced Safety Concepts, Inc. (ASCI), Santa Fe, NM (United States)

    2018-01-22

    The purpose of this small business CRADA was to evaluate the performance of new airbag concepts which were developed by the Advanced Safety Concepts, Inc. (ASCI). These new airbag concepts, if successful, could have major potential savings to society in terms of fewer injuries, lost time and lives.

  3. Development, Field Test, and Refinement of Performance Training Programs in Armor Advanced Individual Training. Final Report.

    Science.gov (United States)

    Young, Douglas L.; Taylor, John E.

    Performance-oriented instruction was developed, field tested, and refined in two Advanced Individual Training (AIT) programs--Armor Reconnaissance Specialist (MOS 11D) and Armor Crewman (MOS 11E). Tasks for both MOS (Military Occupational Specialty) were inventoried and the inventories were reduced by eliminating those tasks which are not required…

  4. Final report of DOE project "Detection, Localization and Diagnosis of Performance Problems Using PerfSONAR"

    Energy Technology Data Exchange (ETDEWEB)

    Dovrolis, Konstantinos [Georgia Tech

    2014-04-15

    We present the development of a middleware service, called Pythia, that is able to detect, localize, and diagnose performance problems in the network paths that interconnect research sites that are of interest to DOE. The proposed service can analyze perfSONAR data collected from all participating sites.

  5. Pertes en eau : introduction de la pression dans les indicateurs de performance : rapport final

    OpenAIRE

    Renaud, E.; Pillot, J.

    2013-01-01

    / En France, un système d'indicateurs de performance des réseaux d'eau potable est fixe par la réglementation. En matière d'évaluation des pertes, ce système rapporte le volume annuel perdu au volume annuel consomme ou a la longueur des canalisations mais aucun indicateur ne prend en compte la pression ou le nombre de branchements. A l'inverse, le système d'indicateurs de performance de l'IWA prend en compte ces paramètres, cependant, des études antérieures ont montre que son application est ...

  6. A Final Review of the Performance of the CDF Run II Data Acquisition System

    CERN Multimedia

    CERN. Geneva

    2012-01-01

    The CDF Collider Detector at Fermilab ceased data collection on September 30, 2011 after over twenty five years of operation. We review the performance of the CDF Run II data acquisition systems over the last ten of these years while recording nearly 10 fb-1 of proton-antiproton collisions with a high degree of efficiency. Technology choices in the online control and configuration systems and front-end embedded processing have impacted the efficiency and quality of the data accumulated by CDF, and have had to perform over a large range of instantaneous luminosity values and trigger rates. We identify significant sources of problems and successes. In particular, we present our experience computing and acquiring data in a radiation environment, and attempt to correlate system technical faults with radiation dose rate and technology choices.

  7. Highly loaded multi-stage fan drive turbine: Performance of final three configurations

    Science.gov (United States)

    Cherry, D. G.; Thomas, M. W.

    1974-01-01

    Results for a three-stage highly loaded fan drive turbine follow-on test program are presented. The effects of combinations of tandem and leaned bladerows on three-stage turbine performance were tested. The three-stage turbine with a tandem stator in stage two exhibited a total-to-total efficiency of approximately 0.887 as compared to 0.886 for the plain blade turbine base case.

  8. Final Technical Report - Recovery Act: Organic Coatings as Encapsulants for Low Cost, High Performance PV Modules

    Energy Technology Data Exchange (ETDEWEB)

    Stuart Hellring; Jiping Shao; James Poole

    2011-12-05

    The objective of this project was to evaluate the feasibility of utilizing PPG's commercial organic coatings systems as efficient, modernized encapsulants for low cost, high performance, thin film photovoltaic modules. Our hypothesis was that the combination of an anticorrosive coating with a more traditional barrier topcoat would mitigate many electrochemical processes that are now responsible for the significant portion of photovoltaic (PV) failures, thereby nullifying the extremely high moisture barrier requirements of currently used encapsulation technology. Nine commercially available metal primer coatings and six commercially available top coatings were selected for screening. Twenty-one different primer/top coat combinations were evaluated. The primer coatings were shown to be the major contributor to corrosion inhibition, adhesion, and barrier properties. Two primer coatings and one top coating were downselected for testing on specially-fabricated test modules. The coated test modules passed initial current leakage and insulation testing. Damp Heat testing of control modules showed visible corrosion to the bus bar metal, whereas the coated modules showed none. One of the primer/top coat combinations retained solar power performance after Damp Heat testing despite showing some delamination at the EVA/solar cell interface. Thermal Cycling and Humidity Freeze testing resulted in only one test module retaining its power performance. Failure modes depended on the particular primer/top coating combination used. Overall, this study demonstrated that a relatively thin primer/top coating has the potential to replace the potting film and backsheet in crystalline silicon-based photovoltaic modules. Positive signals were received from commercially available coatings developed for applications having performance requirements different from those required for photovoltaic modules. It is likely that future work to redesign and customize these coatings would result in

  9. High Density, Insensitive Oxidizer With RDX Performance Final Report CRADA No. TC02178.0

    Energy Technology Data Exchange (ETDEWEB)

    Pagoria, P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Preda, D. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2017-08-25

    This was a collaborative effort between Lawrence Livermore National Security, LLC as manager and operator of Lawrence Livermore National Laboratory (LLNL) and Physical Sciences, Inc. (PSI), to develop a synthesis and evaluate a novel high density, insensitive oxidizer with RDX performance. This CRADA resulted from the award of a Phase I STTR ("STTR") from DOD. In recent years, the synthesis of new energetic heterocyclic compounds to replace the energetic materials currently in the stockpile has received a great amount of attention. The Office of the Secretary of Defense has identified that there is a need to incorporate new energetic materials in current and future weapon systems in an effort to increase performance and decrease sensitivity. For many of the future weapon systems, incorporation of energetic compounds currently in the stockpile will not provide the desired performance and sensitivity goals. The success of this CRADA may lead to a Phase I option STTR from DOD and to a Phase II STTR from DOD. The goal of this CRADA was to produce and test a novel oxidizer, 2,5,8-trinitroheptazine (TNH).

  10. Performance measurement of the gas tax and public transit funds : final report

    International Nuclear Information System (INIS)

    2007-01-01

    Federal funding for the gas tax fund and public transit fund are provided through Infrastructure Canada for municipal infrastructure across Canada in a broad range of municipal service projects. In order to identify appropriate outcomes that would meet reporting requirements for the gas tax fund and public transit fund, this report outlined a performance measurement approach that would allow for the reporting of projects under both funds and provide a structured methodology for multiple year analysis of benefits. The report discussed the performance measures process review and outcomes approach logic model. It also provided an outline of information sourcing strategies including an overview of the project types and expenditures; information sourcing strategy; typical municipal information sources by project type; performance measurement framework assumptions and limitations; and modeling of outcomes from outputs. Conclusions and recommendations were also offered. It was concluded that based on a comprehensive review of ancillary benefits and outcomes of various historic funding programs, there are 3 foundational outcomes that should be considered to assess all initial program outcomes. These include cleaner air, cleaner water and reduced greenhouse gas emissions. tabs., figs

  11. Periodic usage of low-protein methionine-fortified diets in broiler chickens under high ambient temperature conditions: effects on performance, slaughter traits, leukocyte profiles and antibody response

    Science.gov (United States)

    Ghasemi, Hossein Ali; Ghasemi, Rohollah; Torki, Mehran

    2014-09-01

    This study was performed to evaluate the effects of adding methionine supplements to low-protein diets and subsequent re-feeding with a normal diet on the productive performance, slaughter parameters, leukocyte profiles and antibody response in broiler chickens reared under heat stress conditions. During the whole experimental period (6-49 days), the birds were raised in battery cages located in high ambient temperature in an open-sided housing system. A total of 360 6-day-old male chickens were divided into six treatments in six replicates with ten chicks each. Six isoenergetic diets, with similar total sulfur amino acids levels, were formulated to provide 100 and 100 (control), 85 and 100 (85S), 70 and 100 (70S), 85 and 85 (85SG), 70 and 85 (70S85G), and 70 and 70 % (70SG) of National Research Council recommended levels for crude protein during the starter (6-21 day) and grower (22-42 day) periods, respectively. Subsequently, all groups received a diet containing the same nutrients during the finisher period (43-49 day). The results showed that, under heat stress conditions, average daily gain and feed conversion ratio and performance index from day 6 to 49, breast and thigh yields and antibody titer against Newcastle disease in the birds fed diets 85S, 70S and 85SG were similar to those of birds fed control diet, whereas feeding diets 70S85G and 70SG significantly decreased the values of above-mentioned parameters. Additionally, diets 85S, 70S and 85SG significantly decreased mortality rate and heterophil:lymphocyte ratio compared with the control diet. In conclusion, the results indicate that supplementation of methionine to diets 85S, 70S and 85SG, and then re-feeding with a conventional diet is an effective tool to maintain productive performance and to improve health indices and heat resistance in broilers under high ambient temperature conditions.

  12. Advanced Insulation for High Performance Cost-Effective Wall, Roof, and Foundation Systems Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Costeux, Stephane [Dow Chemical Company, Midland, MI (United States); Bunker, Shanon [Dow Chemical Company, Midland, MI (United States)

    2013-12-20

    The objective of this project was to explore and potentially develop high performing insulation with increased R/inch and low impact on climate change that would help design highly insulating building envelope systems with more durable performance and lower overall system cost than envelopes with equivalent performance made with materials available today. The proposed technical approach relied on insulation foams with nanoscale pores (about 100 nm in size) in which heat transfer will be decreased. Through the development of new foaming methods, of new polymer formulations and new analytical techniques, and by advancing the understanding of how cells nucleate, expand and stabilize at the nanoscale, Dow successfully invented and developed methods to produce foams with 100 nm cells and 80% porosity by batch foaming at the laboratory scale. Measurements of the gas conductivity on small nanofoam specimen confirmed quantitatively the benefit of nanoscale cells (Knudsen effect) to increase insulation value, which was the key technical hypotheses of the program. In order to bring this technology closer to a viable semi-continuous/continuous process, the project team modified an existing continuous extrusion foaming process as well as designed and built a custom system to produce 6" x 6" foam panels. Dow demonstrated for the first time that nanofoams can be produced in a both processes. However, due to technical delays, foam characteristics achieved so far fall short of the 100 nm target set for optimal insulation foams. In parallel with the technology development, effort was directed to the determination of most promising applications for nanocellular insulation foam. Voice of Customer (VOC) exercise confirmed that demand for high-R value product will rise due to building code increased requirements in the near future, but that acceptance for novel products by building industry may be slow. Partnerships with green builders, initial launches in smaller markets (e.g. EIFS

  13. Final Technical Report, Oct 2004 - Nov. 2006, High Performance Flexible Reversible Solid Oxide Fuel Cell

    Energy Technology Data Exchange (ETDEWEB)

    Guan, Jie; Minh, Nguyen

    2007-02-21

    This report summarizes the work performed for the program entitled “High Performance Flexible Reversible Solid Oxide Fuel Cell” under Cooperative Agreement DE-FC36-04GO14351 for the U. S. Department of Energy. The overall objective of this project is to demonstrate a single modular stack that generates electricity from a variety of fuels (hydrogen and other fuels such as biomass, distributed natural gas, etc.) and when operated in the reverse mode, produces hydrogen from steam. This project has evaluated and selected baseline cell materials, developed a set of materials for oxygen and hydrogen electrodes, and optimized electrode microstructures for reversible solid oxide fuel cells (RSOFCs); and demonstrated the feasibility and operation of a RSOFC multi-cell stack. A 10-cell reversible SOFC stack was operated over 1000 hours alternating between fuel cell (with hydrogen and methane as fuel) and steam electrolysis modes. The stack ran very successfully with high power density of 480 mW/cm2 at 0.7V and 80% fuel utilization in fuel cell mode and >6 SLPM hydrogen production in steam electrolysis mode using about 1.1 kW electrical power. The hydrogen generation is equivalent to a specific capability of 2.59 Nm3/m2 with electrical energy demand of 3 kWh/Nm3. The performance stability in electrolysis mode was improved vastly during the program with a degradation rate reduction from 8000 to 200 mohm-cm2/1000 hrs. This was accomplished by increasing the activity and improving microstructure of the oxygen electrode. Both cost estimate and technology assessment were conducted. Besides the flexibility running under both fuel cell mode and electrolysis mode, the reversible SOFC system has the potentials for low cost and high efficient hydrogen production through steam electrolysis. The cost for hydrogen production at large scale was estimated at ~$2.7/kg H2, comparing favorably with other electrolysis techology.

  14. Design and performance of the Georgia Tech Aquatic Center photovoltaic system. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Rohatgi, A.; Begovic, M.; Long, R.; Ropp, M.; Pregelj, A.

    1996-12-31

    A building-integrated DC PV array has been constructed on the Georgia Tech campus. The array is mounted on the roof of the Georgia Tech Aquatic Center (GTAC), site of the aquatic events during the 1996 Paralympic and Olympic Games in Atlanta. At the time of its construction, it was the world`s largest roof-mounted photovoltaic array, comprised of 2,856 modules and rates at 342 kW. This section describes the electrical and physical layout of the PV system, and the associated data acquisition system (DAS) which monitors the performance of the system and collects measurements of several important meteorological parameters.

  15. High performance ground penetrating radar survey of TA-49/Area 2. Final report

    International Nuclear Information System (INIS)

    Hoeberling, R.F.; Rangel, M.J. III

    1994-09-01

    The results of high performance ground penetrating radar study of Area 2 at Technical Area 49 are presented. The survey was commissioned as part of Los Alamos Laboratory's continuing Environmental Remediation program and was completed and analyzed before borehole studies in Area 2 were started. Based upon the ground penetrating radar results, the location of one of the planned boreholes was moved to assure the drilling area was as safe as possible. While earlier attempts to use commercial radar devices at this facility had not been successful, the radar and digital processing system developed at Los Alamos were able to significantly improve the buried physical detail of the site

  16. Final Qualification and Early On-Orbit Performance of the HESSI Cryocooler

    Science.gov (United States)

    Boyle, R. F.; Banks, I. S.; Shirey, K.

    2002-01-01

    The High Energy Solar Spectroscopic Imager (HESSI) spacecraft was launched on February 5, 2002. It now observes the Sun with the finest angular and energy resolutions ever achieved from a few keV to hundreds of keV, using an array of nine germanium detectors operating at 75K. The spacecraft was originally scheduled for launch in July 2000, but a vibration facility mishap damaged the primary structure of the spacecraft, along with the cryocooler, This paper describes issues in the qualification of a replacement for the original flight cooler, and describes early on-orbit performance.

  17. Final Report - High Performance, Durable, Low Cost Membrane Electrode Assemblies for Transportation Applications

    Energy Technology Data Exchange (ETDEWEB)

    Steinbach, Andrew [3M Company, Maplewood, MN (United States)

    2017-05-31

    The primary project objective was development of improved polymer electrolyte membrane fuel cell (PEMFC) membrane electrode assemblies (MEAs) which address the key DOE barriers of performance, durability and cost. Additional project objectives were to address commercialization barriers specific to MEAs comprising 3M nanostructured thin film (NSTF) electrodes, including a larger-than-acceptable sensitivity to operating conditions, an unexplained loss of rated power capability with operating time, and slow break-in conditioning. Significant progress was made against each of these barriers, and most DOE 2020 targets were met or substantially approached.

  18. High Performance Fuel Laboratory, Hanford Reservation, Richland, Washington. Final environmental impact statement

    International Nuclear Information System (INIS)

    1977-09-01

    The High Performance Fuel Laboratory (HPFL) will provide pilot scale tests of manufacturing processes, equipment, and handling systems and of accountability and safeguards, methods, and equipment while keeping radiological and chemical exposures of the workers, public, and environment at the lowest practicable levels. The experience gained from designing, constructing and operating the HPFL can be used in future commitments to commercial fuel fabrication plants in the late 1980s and beyond for processing of nuclear fuel. The HPFL site is located in the 400 Area of the 559-square mile, federally owned Hanford Reservation. This environmental impact statement considers effects of the HPFL under normal conditions and in the event of an accident

  19. Feed-pump hydraulic performance and design improvement, Phase I: research program design. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Brown, W.H.; Gopalakrishnan, S.; Fehlau, R.; Thompson, W.E.; Wilson, D.G.

    1982-03-01

    As a result of prior EPRI-sponsored studies, it was concluded that a research program should be designed and implemented to provide an improved basis for the design, procurement, testing, and operation of large feed pumps with increased reliability and stability over the full range of operating conditions. This two-volume report contains a research plan which is based on a review of the present state of the art and which defines the necessary R and D program and estimates the benefits and costs of the program. The recommended research program consists of 30 interrelated tasks. It is designed to perform the needed research; to verify the results; to develop improved components; and to publish computer-aided design methods, pump specification guidelines, and a troubleshooting manual. Most of the technology proposed in the research plan is applicable to nuclear power plants as well as to fossil-fired plants. This volume contains appendixes on pump design, cavitation damage, performance testing, hydraulics, two-phase flow in pumps, flow stability, and rotor dynamics.

  20. Application of solar energy to industrial drying of soybeans: Phase III, performance evaluation. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Hall, B.R.

    1979-10-31

    A 15-month performance evaluation was conducted on a solar system designed and constructed to augment the industrial drying of soybeans at the Gold Kist, Inc., extraction plant in Decatur, Alabama. The plant employs three oil-fired, continuous-flow dryers of 3,000 bu/hr each. The solar system consists of 672 Solaron air collectors that temper the airflow into the existing dryers. Since the requirement for energy exceeds the peak solar system capacity, no storage is provided. The interface with the existing facility is simply accomplished by three ducts that release the solar heated air directly adjacent to the dryer air intakes, and no mechanical coupling is needed. The solar system was operated for 1,752 hr on 290 days during the 15-month period without a single failure sufficient to cause shutdown. No interference with normal plant operations was experienced. Maintenance of the solar system, consisting of service to the air handling unit, cleaning of collector glazing, and minor duct repair, totaled $1,564. System utilization was only 46.3%. This was primarily due to daytime routine maintenance performed on the conventional drying and processing equipment. The solar fraction was not large enough to justify maintenance shift changes. An average collector efficiency of 26.2% was experienced. Contamination caused by the local plant environment reduced the average collector efficiency by 9.3 percentage points. A prototype of an automatic cleaning system was constructed and tested.

  1. Feed-pump hydraulic performance and design improvement, Phase I: research program design. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Brown, W.H.; Gopalakrishnan, S.; Fehlau, R.; Thompson, W.E.; Wilson, D.G.

    1982-03-01

    As a result of prior EPRI-sponsored studies, it was concluded that a research program should be designed and implemented to provide an improved basis for the design, procurement, testing, and operation of large feed pumps with increased reliability and stability over the full range of operating conditions. This two-volume report contains a research plan which is based on a review of the present state of the art and which defines the necessary R and D program and estimates the benefits and costs of the program. The recommended research program consists of 30 interrelated tasks. It is designed to perform the needed research; to verify the results; to develop improved components; and to publish computer-aided design methods, pump specification guidelines, and a troubleshooting manual. Most of the technology proposed in the research plan is applicable to nuclear power plants as well as to fossil-fired plants. This volume discusses the design, performance and failures of feed pumps, and recommendations for research on pump dynamics, design, and specifications.

  2. Investigation of properties and performance of ceramic composite components: Final report on Phases 3 and 4

    Energy Technology Data Exchange (ETDEWEB)

    Curtin, W.A.; Halverson, H.; Carter, R.H.; Miraj, N.; Reifsnider, K.L. [Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (United States)

    1998-01-15

    The objective of the Fossil Energy Advanced Research and Technology Development (AR and TD) Materials Program is to conduct research and development on materials for longer-term fossil energy applications as well as for generic needs of various fossil fuel technologies. The research program of the Materials Response Group at Virginia Tech addresses the need for reliable and durable structural ceramic composites to perform in high temperature environments. The research effort provides an experimental and analytical basis for the transition from properties of materials to performance of actual component structures. Phases 1 and 2 of the present program focused on the development of test capabilities, initial studies of component mechanical response under various conditions and the development of a life prediction methodology. These efforts have been described in previous reports. This report summarizes the major tasks completed under Phases 3 and 4 of the project. Overall, the authors have made significant progress in a broad spectrum of tasks in this program. Their efforts have encompassed component evaluation, assessment of new SiC-based composites with improved high-temperature potential, development of oxide coating materials for SiC, and the extension and development of new models for predicting the durability of composite components under specific operating conditions for various CMC applications. Each of these areas of work is an important area for achieving the ultimate goal of usable SiC-based composites in high-temperature corrosive environments typical of fossil energy applications.

  3. LIFAC sorbent injection desulfurization demonstration project. Final report, volume II: Project performance and economics

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-01-01

    This publication discusses the demonstration of the LIFAC sorbent injection technology at Richmond Power and Light`s Whitewater Valley Unit No. 2, performed under the auspices of the U.S. Department of Energy`s (DOE) Clean Coal Technology Program. LIFAC is a sorbent injection technology capable of removing 75 to 85 percent of a power plant`s SO{sub 2} emissions using limestone at calcium to sulfur molar ratios of between 2 and 2.5 to 1. The site of the demonstration is a coal-fired electric utility power plant located in Richmond, Indiana. The project is being conducted by LIFAC North America (LIFAC NA), a joint venture partnership of Tampella Power Corporation and ICF Kaiser Engineers, in cooperation with DOE, RP&L, and Research Institute (EPRI), the State of Indiana, and Black Beauty Coal Company. The purpose of Public Design Report Volume 2: Project Performance and Economics is to consolidate, for public use, the technical efficiency and economy of the LIFAC Process. The report has been prepared pursuant to the Cooperative Agreement No. DE-FC22-90PC90548 between LIFAC NA and the U.S. Department of Energy.

  4. Quality not quantity for transglutaminase antibody 2: the performance of an endomysial and tissue transglutaminase test in screening coeliac disease remains stable over time.

    Science.gov (United States)

    Swallow, K; Wild, G; Sargur, R; Sanders, D S; Aziz, I; Hopper, A D; Egner, W

    2013-01-01

    National Institute of Clinical Excellence (NICE) and European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) guidance for the diagnosis of coeliac disease has been published. However, there is some controversy regarding the advice on the use of stratifying levels of immunoglobulin (IgA) tissue transglutaminase antibody (TG2) test positivity in the absence of test standardization and the vagueness of the indication to test equivocal samples. Using repeat service audit, we demonstrate that a combination of TG2 followed by IgA endomysial antibodies (EMA) is the best strategy for all degrees of mucosal abnormality using our test combination. Reliance upon immunoassay titre is not as effective, and cannot be applied consistently across populations in the absence of assay standardization. Guidelines advocating the use of tests should involve experts in laboratory diagnostics and external quality assurance to ensure that errors of generalization do not occur and that test performance is achievable in routine diagnostic use. © 2012 British Society for Immunology.

  5. Foundation Heat Exchanger Final Report: Demonstration, Measured Performance, and Validated Model and Design Tool

    Energy Technology Data Exchange (ETDEWEB)

    Hughes, Patrick [Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Im, Piljae [Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

    2012-01-01

    (FHX) has been coined to refer exclusively to ground heat exchangers installed in the overcut around the basement walls. The primary technical challenge undertaken by this project was the development and validation of energy performance models and design tools for FHX. In terms of performance modeling and design, ground heat exchangers in other construction excavations (e.g., utility trenches) are no different from conventional HGHX, and models and design tools for HGHX already exist. This project successfully developed and validated energy performance models and design tools so that FHX or hybrid FHX/HGHX systems can be engineered with confidence, enabling this technology to be applied in residential and light commercial buildings. The validated energy performance model also addresses and solves another problem, the longstanding inadequacy in the way ground-building thermal interaction is represented in building energy models, whether or not there is a ground heat exchanger nearby. Two side-by-side, three-level, unoccupied research houses with walkout basements, identical 3,700 ft{sup 2} floor plans, and hybrid FHX/HGHX systems were constructed to provide validation data sets for the energy performance model and design tool. The envelopes of both houses are very energy efficient and airtight, and the HERS ratings of the homes are 44 and 45 respectively. Both houses are mechanically ventilated with energy recovery ventilators, with space conditioning provided by water-to-air heat pumps with 2 ton nominal capacities. Separate water-to-water heat pumps with 1.5 ton nominal capacities were used for water heating. In these unoccupied research houses, human impact on energy use (hot water draw, etc.) is simulated to match the national average. At House 1 the hybrid FHX/HGHX system was installed in 300 linear feet of excavation, and 60% of that was construction excavation (needed to construct the home). At House 2 the hybrid FHX/HGHX system was installed in 360 feet of

  6. Foundation Heat Exchanger Final Report: Demonstration, Measured Performance, and Validated Model and Design Tool

    Energy Technology Data Exchange (ETDEWEB)

    Hughes, Patrick [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Im, Piljae [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2012-04-01

    (FHX) has been coined to refer exclusively to ground heat exchangers installed in the overcut around the basement walls. The primary technical challenge undertaken by this project was the development and validation of energy performance models and design tools for FHX. In terms of performance modeling and design, ground heat exchangers in other construction excavations (e.g., utility trenches) are no different from conventional HGHX, and models and design tools for HGHX already exist. This project successfully developed and validated energy performance models and design tools so that FHX or hybrid FHX/HGHX systems can be engineered with confidence, enabling this technology to be applied in residential and light commercial buildings. The validated energy performance model also addresses and solves another problem, the longstanding inadequacy in the way ground-building thermal interaction is represented in building energy models, whether or not there is a ground heat exchanger nearby. Two side-by-side, three-level, unoccupied research houses with walkout basements, identical 3,700 ft{sup 2} floor plans, and hybrid FHX/HGHX systems were constructed to provide validation data sets for the energy performance model and design tool. The envelopes of both houses are very energy efficient and airtight, and the HERS ratings of the homes are 44 and 45 respectively. Both houses are mechanically ventilated with energy recovery ventilators, with space conditioning provided by water-to-air heat pumps with 2 ton nominal capacities. Separate water-to-water heat pumps with 1.5 ton nominal capacities were used for water heating. In these unoccupied research houses, human impact on energy use (hot water draw, etc.) is simulated to match the national average. At House 1 the hybrid FHX/HGHX system was installed in 300 linear feet of excavation, and 60% of that was construction excavation (needed to construct the home). At House 2 the hybrid FHX/HGHX system was installed in 360 feet of

  7. Spent Fuel Performance Assessment and Research. Final Report of a Coordinated Research Project (SPAR-II)

    International Nuclear Information System (INIS)

    2012-01-01

    As storage of spent fuel has become a key technology in spent fuel management, wet and dry storage have become mature technologies and continue to demonstrate good performance. Increased spent fuel storage capacity in combination with longer storage durations will be needed over the foreseeable future as many countries have delayed their decision on spent fuel disposal or reprocessing. Extended spent fuel storage is, and will remain, an important activity for all countries with nuclear power programmes. A number of countries are planning or have already initiated research programmes on spent fuel storage performance, and there is a continuing benefit in exchanging spent fuel storage experience of the Member States in order to build a comprehensive technology knowledge base. Potential degradation mechanisms that may affect cladding integrity during wet storage are uniform corrosion, pitting, galvanic, and microbiologically-influenced corrosion. Potential degradation mechanisms that may affect cladding integrity during dry storage and subsequent handling and transportation operations are air oxidation, thermal creep, stress corrosion cracking (SCC), delayed hydride cracking (DHC), hydride re-orientation, hydrogen migration and re-distribution. Investigations carried out so far indicate that from the degradation mechanisms that may affect the integrity of spent fuel assembly/bundle structure during interim storage, hydride re-orientation has the potential to impair the ability of the cladding to effectively withstand potentially adverse mechanical challenges resulting from handling or transportation accidents. Fuel integrity issues are related to the definition and criteria of fuel integrity, failure classification, packaging and retrieval of damaged fuel and transport of damaged fuel assemblies. Various monitoring technologies have been developed and used to confirm the continued spent fuel integrity during storage or to provide an early indication of developing

  8. Behavioral Health and Performance (BHP) Standing Review Panel (SRP) Final Report

    Science.gov (United States)

    Suedfeld, Peter; Bootzin, Richard; Harvey, Allison; Leon, Gloria; Musson, David; Oltmanns, Thomas; Paulus, Martin

    2010-01-01

    The first meeting of the Behavioral Health and Performance (BHP) Standing Review Panel (SRP) was held in Houston, TX on November 1-3, 2009. Our task was to assess the Integrated Research Plan (IRP) related to the fields covered in the SRP charge (see section VIII). Having considered and discussed the extensive materials distributed prior to the meeting, and the highly informative briefings by the NASA BHP Human Research Program (HRP) personnel during the site visit, the SRP agreed that the IRP is comprehensive and was developed carefully. Question and answer periods with the presenters were particularly productive and helpful to the SRP. The presenters' willingness to share information and positive responses to the SRP's suggestions were greatly appreciated. Although the IRP and related documents are impressive, the SRP does have a number of recommendations regarding both the overall plan and its component parts.

  9. Collective Thomson scattering energetic particle diagnostic in high performance tokamaks. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Cheung, P.Y.; Aamodt, R.E.; Russell, D.A.

    1997-07-08

    This report summarizes the work performed under DOE grant DE-FG03-95ER54334. Lodestar was an active participant in the low power Collective Thomson Scattering (CTS) diagnostic experiment at TFTR in collaboration with MIT. A simple and effective fitting technique was developed to extract key parameters from the scattered data. Utilizing this new technique, the concept of lower hybrid resonance scattering was adapted for a feasibility study of a low/medium power collective scattering diagnostic for ITER. The implementation and the testing of such a technique for actual parameter extraction using TFTR data, however, was severely limited due to experimental and instrumentation complications. Based on the studies the authors have performed up to date, it is believed that a combination of non-physics related effects such as multiple wall reflection of incident signal and spectral impurity problem o the gyrotron can account for the anomalous signal strength. A collaborative effort with GA was initiated and a feasibility study of developing and implementing a collective thomson scattering (CTS) diagnostic for the detection of energetic particles at DIII-D was completed. Specifically, the process of selecting an optimum receiver location for the diagnostic is discussed in detailed. Results presented here include detailed signal to noise calculations and ray-tracing studies. Critical physics issues and selection criteria are discussed and a procedure to detect anisotropic energetic ion temperatures is also outlined. Favorable results, obtained in the feasibility study, indicate that it should be possible to develop and implement a CTS diagnostic at DIII-D.

  10. Performance characteristics of rheumatoid factor and anti-cyclic citrullinated peptide antibody assays may impact ACR/EULAR classification of rheumatoid arthritis.

    Science.gov (United States)

    Van Hoovels, Lieve; Jacobs, Julie; Vander Cruyssen, Bert; Van den Bremt, Stefanie; Verschueren, Patrick; Bossuyt, Xavier

    2018-01-23

    Rheumatoid factor (RF) and anti-cyclic citrullinated protein/peptide antibodies (ACPA) are integrated in the 2010 American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) classification criteria for rheumatoid arthritis (RA). The objectives of this study were to evaluate the technical and diagnostic performance of different RF and ACPA assays and to evaluate whether differences in performance impact RA classification. Samples from 594 consecutive patients who for the first time consulted a rheumatologist (44 of whom were diagnosed with RA) and 26 extra newly diagnosed patients with RA were analysed with six different RF assays (Menarini, Thermo Fisher, Inova, Roche, Abbott, Euroimmun) and seven different ACPA assays (Menarini, Thermo Fisher, Inova, Roche, Abbott, Euro Diagnostica, Euroimmun). We found differences in analytical performance between assays. There was poor numerical agreement between the different RF and ACPA assays. For all assays, the likelihood ratio for RA increased with increasing antibody levels. The areas under the curve of receiver operating characteristic analysis of the RF (range 0.676-0.709) and ACPA assays (range 0.672-0.769) only differed between some ACPA assays. Nevertheless, using the cut-off proposed by the manufacturer, there was a large variation in sensitivity and specificity between assays (mainly for RF). Consequently, depending on the assay used, a subgroup of patients (13% for RF, 1% for ACPA and 9% for RF/ACPA) might or might not be classified as RA according to the 2010 ACR/EULAR criteria. Due to poor harmonisation of RF and ACPA assays and of test result interpretation, RA classification according to 2010 ACR/EULAR criteria may vary when different assays are used. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  11. Performance evaluation of four type-specific commercial assays for detection of herpes simplex virus type 1 antibodies in a Middle East and North Africa population.

    Science.gov (United States)

    Aldisi, Rana S; Elsidiq, Malaz S; Dargham, Soha R; Sahara, Afifah S; Al-Absi, Enas S; Nofal, Mariam Y; Mohammed, Layla I; Abu-Raddad, Laith J; Nasrallah, Gheyath K

    2018-03-22

    The number of diagnostic assays for the detection of herpes simplex virus type 1 (HSV-1) antibodies has increased over the years. However, their performance characteristics could vary among global populations. To investigate performance of two commercial ELISA kits, HerpeSelect ® 1 ELISA and Euroimmun Anti-HSV-1 (gC1) ELISA (IgG); and two commercial immunoblot (IB)/Western blot (WB) assays, HerpeSelect ® 1 and 2 Immunoblot IgG, and Euroimmun Anti-HSV-1/HSV-2 gG2 Euroline-WB (IgG/IgM); in detecting HSV-1 antibodies in a Middle East and North Africa (MENA) population. Blood specimens were collected from blood donors in Doha, Qatar, June 2013-2016. Twenty specimens were randomly selected from 10 MENA nationalities (Egypt, Iran, Jordan, Lebanon, Pakistan, Palestine, Qatar, Sudan, Syria, and Yemen; total = 200), and tested for HSV-1 antibodies. Across all six comparisons between assays, positive percent agreement ranged between 95.7% (95% CI: 91.4-98.3%) and 100.0% (95% CI: 97.8-100.0%). Negative percent agreement ranged between 86.2% (95% CI: 68.3-96.1%) and 96.2% (95% CI: 80.4-99.9%). Overall percent agreement ranged between 95.7% (95% CI: 91.7-97.8%) and 99.4% (95% CI: 96.7-99.9%). Cohen's kappa statistic ranged between 0.84 (95% CI: 0.73-0.95) and 0.98 (95% CI: 0.93-1.00). Compared against IB/WB, HerpeSelect ® and Euroimmun had sensitivities and specificities >96% and >86%, respectively. Positive and negative predictive values were >97% and >83%, respectively. The assays showed excellent concordance with one another, and with a high kappa statistic. The ELISA kits demonstrated robust diagnostic performance compared to the IB/WB assays. These findings support the assays' utility in clinical diagnosis and research in MENA populations. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

  12. Coordinated Fault-Tolerance for High-Performance Computing Final Project Report

    Energy Technology Data Exchange (ETDEWEB)

    Panda, Dhabaleswar Kumar [The Ohio State University; Beckman, Pete

    2011-07-28

    existing publish-subscribe tools. We enhanced the intrinsic fault tolerance capabilities representative implementations of a variety of key HPC software subsystems and integrated them with the FTB. Targeting software subsystems included: MPI communication libraries, checkpoint/restart libraries, resource managers and job schedulers, and system monitoring tools. Leveraging the aforementioned infrastructure, as well as developing and utilizing additional tools, we have examined issues associated with expanded, end-to-end fault response from both system and application viewpoints. From the standpoint of system operations, we have investigated log and root cause analysis, anomaly detection and fault prediction, and generalized notification mechanisms. Our applications work has included libraries for fault-tolerance linear algebra, application frameworks for coupled multiphysics applications, and external frameworks to support the monitoring and response for general applications. Our final goal was to engage the high-end computing community to increase awareness of tools and issues around coordinated end-to-end fault management.

  13. Final Technical Report: Affordable, High-Performance, Intermediate Temperature Solid Oxide Fuel Cells

    Energy Technology Data Exchange (ETDEWEB)

    Blackburn, Bryan [Redox Power Systems, LLC; Bishop, Sean [Redox Power Systems, LLC; Gore, Colin [Redox Power Systems, LLC; Wang, Lei [Redox Power Systems, LLC; Correa, Luis [Redox Power Systems, LLC; Langdo, Thomas [Redox Power Systems, LLC; Deaconu, Stelu [Redox Power Systems, LLC; Pan, Keji [Redox Power Systems, LLC

    2018-02-15

    In this project, we improved the power output and voltage efficiency of our intermediate temperature solid oxide fuel cells (IT-SOFCs) with a focus on ~600 °C operation. At these temperatures and with the increased power density (i.e., fewer cells for same power output), the stack cost should be greatly reduced while extending durability. Most SOFC stacks operate at temperatures greater than 800 °C. This can greatly increase the cost of the system (stacks and BOP) as well as maintenance costs since the most common degradation mechanisms are thermally driven. Our approach uses no platinum group metal (PGM) materials and the lower operating temperature allows use of simple stainless steel interconnects and commercial off-the-shelf gaskets in the stack. Furthermore, for combined heating and power (CHP) applications the stack exhaust still provides “high quality” waste heat that can be recovered and used in a chiller or boiler. The anticipated performance, durability, and resulting cost improvements (< $700/kWe) will also move us closer to reaching the full potential of this technology for distributed generation (DG) and residential/commercial CHP. This includes eventual extension to cleaner, more efficient portable generators, auxiliary power units (APUs), and range extenders for transportation. The research added to the understanding of the area investigated by exploring various methods for increasing power density (Watts/square centimeter of active area in each cell) and increasing cell efficiency (increasing the open circuit voltage, or cell voltage with zero external electrical current). The results from this work demonstrated an optimized cell that had greater than 1 W/cm2 at 600 °C and greater than 1.6 W/cm2 at 650 °C. This was demonstrated in large format sizes using both 5 cm by 5 cm and 10 cm by 10 cm cells. Furthermore, this work demonstrated that high stability (no degradation over > 500 hours) can be achieved together with high performance in large

  14. Sewage Sludge Incinerators: Final Standards of Performance for New Stationary Sources and Emission Guidelines for Existing Sources Final Rule Fact Sheets

    Science.gov (United States)

    This page contains a February 2011 fact sheet with information regarding the final NSPS and Emission Guidelines for Existing Sources for Sewage Sludge Incinerators (SSI). This document provides a summary of the information for these regulations.

  15. Final Phase Flight Performance and Touchdown Time Assessment of TDV in RLV-TD HEX-01 Mission

    Science.gov (United States)

    Yadav, Sandeep; Jayakumar, M.; Nizin, Aziya; Kesavabrahmaji, K.; Shyam Mohan, N.

    2017-12-01

    RLV-TD HEX-01 mission was configured as a precursor flight to actual two stages to orbit vehicle. In this mission RLV-TD was designed as a two stage vehicle for demonstrating the hypersonic flight of a winged body vehicle at Mach No. 5. One of the main objectives of this mission was to generate data for better understanding of new technologies required to design the future vehicle. In this mission, the RLV-TD vehicle was heavily instrumented to get data related to performance of different subsystems. As per the mission design, RLV-TD will land in sea after flight duration of 700 s and travelling a distance of nearly 500 km in Bay of Bengal from the launch site for a nominal trajectory. The visibility studies for telemetry data of vehicle for the nominal and off nominal trajectories were carried out. Based on that, three ground stations were proposed for the telemetry data reception (including one in sea). Even with this scheme it was seen that during the final phase of the flight there will not be any ground station visible to the flight due to low elevation. To have the mission critical data during final phase of the flight, telemetry through INSAT scheme was introduced. During the end of the mission RLV-TD will be landing in the sea on a hypothetical runway. To know the exact time of touchdown for the flight in sea, there was no direct measurement available. Simultaneously there were all chances of losing ground station visibility just before touchdown, making it difficult to assess flight performance during that phase. In this work, telemetry and instrumentation scheme of RLV-TD HEX-01 mission is discussed with an objective to determine the flight performance during the final phase. Further, using various flight sensor data the touchdown time of TDV is assessed for this mission.

  16. Performance of an automated solid-phase red cell adherence system compared with that of a manual gel microcolumn assay for the identification of antibodies eluted from red blood cells.

    Science.gov (United States)

    Finck, R H; Davis, R J; Teng, S; Goldfinger, D; Ziman, A F; Lu, Q; Yuan, S

    2011-01-01

    IgG antibodies coating red blood cells (RBCs) can be removed by elution procedures and their specificity determined by antibody identification studies. Although such testing is traditionally performed using the tube agglutination assay, prior studies have shown that the gel microcolumn (GMC) assay may also be used with comparable results. The purpose of this study was to compare an automated solid-phase red cell adherence (SPRCA) system with a GMC assay for the detection of antibodies eluted from RBCs. Acid eluates from 51 peripheral blood (PB) and 7 cord blood (CB) samples were evaluated by both an automated SPRCA instrument and a manual GMC assay. The concordance rate between the two systems for peripheral RBC samples was 88.2 percent (45 of 51), including cases with alloantibodies (n = 8), warm autoantibodies (n = 12), antibodies with no identifiable specificity (n = 2), and negative results (n = 23). There were six discordant cases, of which four had alloantibodies (including anti-Jka, -E, and -e) demonstrable by the SPRCA system only. In the remaining 2 cases, anti-Fya and antibodies with no identifiable specificity were demonstrable by the GMC assay only. All seven CB specimens produced concordant results, showing anti-A (n = 3), -B (n = 1), maternal anti-Jka (n = 2), or a negative result (n = 1). Automated SPRCA technology has a performance that is comparable with that of a manual GMC assay for identifying antibodies eluted from PB and CB RBCs.

  17. Performance Prediction for Large-Scale Nuclear Waste Repositories: Final Report

    International Nuclear Information System (INIS)

    Glassley, W E; Nitao, J J; Grant, W; Boulos, T N; Gokoffski, M O; Johnson, J W; Kercher, J R; Levatin, J A; Steefel, C I

    2001-01-01

    The goal of this project was development of a software package capable of utilizing terascale computational platforms for solving subsurface flow and transport problems important for disposal of high level nuclear waste materials, as well as for DOE-complex clean-up and stewardship efforts. We sought to develop a tool that would diminish reliance on abstracted models, and realistically represent the coupling between subsurface fluid flow, thermal effects and chemical reactions that both modify the physical framework of the rock materials and which change the rock mineralogy and chemistry of the migrating fluid. Providing such a capability would enhance realism in models and increase confidence in long-term predictions of performance. Achieving this goal also allows more cost-effective design and execution of monitoring programs needed to evaluate model results. This goal was successfully accomplished through the development of a new simulation tool (NUFT-C). This capability allows high resolution modeling of complex coupled thermal-hydrological-geochemical processes in the saturated and unsaturated zones of the Earth's crust. The code allows consideration of virtually an unlimited number of chemical species and minerals in a multi-phase, non-isothermal environment. Because the code is constructed to utilize the computational power of the tera-scale IBM ASCI computers, simulations that encompass large rock volumes and complex chemical systems can now be done without sacrificing spatial or temporal resolution. The code is capable of doing one-, two-, and three-dimensional simulations, allowing unprecedented evaluation of the evolution of rock properties and mineralogical and chemical change as a function of time. The code has been validated by comparing results of simulations to laboratory-scale experiments, other benchmark codes, field scale experiments, and observations in natural systems. The results of these exercises demonstrate that the physics and chemistry

  18. Performance assessment and adoption processes of an information monitoring and diagnostic system prototype; FINAL

    International Nuclear Information System (INIS)

    Piette, Mary Ann

    1999-01-01

    This report addresses the problem that buildings do not perform as well as anticipated during design. We partnered with an innovative building operator to evaluate a prototype Information Monitoring and Diagnostic System (IMDS). The IMDS consists of high-quality measurements archived each minute, a data visualization tool, and a web-based capability. The operators recommend similar technology be adopted in other buildings. The IMDS has been used to identify and correct a series of control problems. It has also allowed the operators to make more effective use of the building control system, freeing up time to take care of other tenant needs. They believe they have significantly improved building comfort, potentially improving tenant health, and productivity. The reduction in hours to operate the building are worth about$20,000 per year, which could pay for the IMDS in about five years. A control system retrofit based on findings from the IMDS is expected to reduce energy use by 20 percent over the next year, worth over$30,000 per year. The main conclusion of the model-based chiller fault detection work is that steady-state models can be used as reference models to monitor chiller operation and detect faults. The ability of the IMDS to measure cooling load and chiller power to one-percent accuracy with a one-minute sampling interval permits detection of additional faults. Evolutionary programming techniques were also evaluated, showing promise in the detection of patterns in building data. We also evaluated two technology adoption processes, radical and routine. In routine adoption, managers enhance features of existing products that are already well understood. In radical adoption, innovative building managers introduce novel technology into their organizations without using the rigorous payback criteria used in routine innovations

  19. Advanced Flue Gas Desulfurization (AFGD) demonstration project: Volume 2, Project performance and economics. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-04-30

    The project objective is to demonstrate removal of 90--95% or more of the SO{sub 2} at approximately one-half the cost of conventional scrubbing technology; and to demonstrate significant reduction of space requirements. In this project, Pure Air has built a single SO{sub 2} absorber for a 528-MWe power plant. The absorber performs three functions in a single vessel: prequencher, absorber, and oxidation of sludge to gypsum. Additionally, the absorber is of a co- current design, in which the flue gas and scrubbing slurry move in the same direction and at a relatively high velocity compared to conventional scrubbers. These features all combine to yield a state- of-the-art SO{sub 2} absorber that is more compact and less expensive than conventional scrubbers. The project incorporated a number of technical features including the injection of pulverized limestone directly into the absorber, a device called an air rotary sparger located within the base of the absorber, and a novel wastewater evaporation system. The air rotary sparger combines the functions of agitation and air distribution into one piece of equipment to facilitate the oxidation of calcium sulfite to gypsum. Additionally, wastewater treatment is being demonstrated to minimize water disposal problems inherent in many high-chloride coals. Bituminous coals primarily from the Indiana, Illinois coal basin containing 2--4.5% sulfur were tested during the demonstration. The Advanced Flue Gas Desulfurization (AFGD) process has demonstrated removal of 95% or more of the SO{sub 2} while providing a commercial gypsum by-product in lieu of solid waste. A portion of the commercial gypsum is being agglomerated into a product known as PowerChip{reg_sign} gypsum which exhibits improved physical properties, easier flowability and more user friendly handling characteristics to enhance its transportation and marketability to gypsum end-users.

  20. High density turbulent plasma processes from a shock tube. Final performance report

    International Nuclear Information System (INIS)

    Johnson, J.A. III.

    1997-01-01

    A broad-based set of measurements has begun on high density turbulent plasma processes. This includes determinations of new plasma physics and the initiation of work on new diagnostics for collisional plasmas as follows: (1) A transient increase is observed in both the spectral energy decay rate and the degree of chaotic complexity at the interface of a shock wave and a turbulent ionized gas. Even though the gas is apparently brought to rest by the shock wave, no evidence is found either of prompt relaminarization or of any systematic influence of end-wall material thermal conductivities on the turbulence parameters. (2) Point fluorescence emissions and averaged spectral line evolutions in turbulent plasmas produced in both the primary and the reflected shock wave flows exhibit ergodicity in the standard turbulence parameters. The data show first evidence of a reverse energy cascade in the collisional turbulent plasma. This suggests that the fully turbulent environment can be described using a stationary state formulation. In these same data, the author finds compelling evidence for a turbulent Stark effect on neutral emission lines in these data which is associated with evidence of large coherent structures and dominant modes in the Fourier analyses of the fluctuations in the optical spectra. (3) A neutral beam generator has been assembled by coupling a Colutron Ion Gun to a charge exchange chamber. Beam-target collisions where the target species is neutral and the beam is either singly charged or neutral have been performed using argon as the working gas. Spectral analysis of the emission shows specific radiative transitions characteristic of both Ar I and Ar II, indicating that some ionization of the target gas results. Gas and plasma parameters such as density, pressure, temperature and flow velocity and their fluctuations can now be followed in real time by spectroscopic analysis of carefully chosen radiative emissions

  1. Energy performance analysis of electrochromic windows in New York commercial office buildings; FINAL

    International Nuclear Information System (INIS)

    Lee, E.S.; Zhou, L.; Yazdanian, M.; Inkarojrit, V.; Slack, J.; Rubin, M.; Selkowitz, S.E.

    2002-01-01

    A DOE-2.1E energy simulation analysis of a switchable electrochromic (EC) glazing with daylighting controls has been conducted for prototypical office buildings in New York (NY). The modeling included four types of office buildings: ''old'' and ''new'' vintages and large (10,405 m(sup 2) , 112,000 ft(sup 2)) and small (502 m(sup 2), 5400 ft(sup 2)) buildings. Five commercially-available, base case windows with and without interior shades were modeled. Window area varied from 0 to 60% of the exterior floor-to-floor wall area. The electric lighting had either no controls or continuous daylighting controls. The prototypes were modeled in New York City or Buffalo. Energy performance data are given for each of the four perimeter zones. Data are presented as a function of window-to-wall ratio in order to better understand the interactions between (1) electric lighting energy use and daylight admission and (2) solar heat gains and space-conditioning energy use. Maximum and minimum reductions in energy use between the EC glazing and all other base case conditions are also presented. Projected energy use reductions relative to typical specified NY office buildings are presented as an indication of the potential impacts EC glazings might have in retrofit and new construction. The energy and demand reductions provided by EC glazings with daylighting controls relative to what is typically specified in office buildings in NY are quite substantial. EC glazings will also dampen fluctuations in interior daylight levels and window brightness, potentially increasing visual comfort

  2. Reproducibility of image interpretation in immunoscintigraphy performed with indium-111- and iodine-131-labeled OC125 F(ab')2 antibody injected into the same patients

    International Nuclear Information System (INIS)

    Vuillez, J.P.; Peltier, P.; Mayer, J.C.; Dubois, F.; Chetanneau, A.; Fumoleau, P.; Bertrand, A.; Chatal, J.F.

    1991-01-01

    An important criterion for the clinical use of a new imaging technique is the correct reproducibility of interpretation. Forty-six paired immunoscintigraphic examinations were performed on 43 patients with suspected ovarian carcinoma recurrence using F(ab')2 fragments of OC125 antibody labeled first with indium-111 and then with iodine-131. Planar scintigraphy (PS) and emission computed tomography (ECT) images were interpreted blindly and separately by three observers, and reproducibility was evaluated by a kappa concordance index. Intra- and interobserver reproducibility were generally satisfactory (kappa values of 0.6 and 0.7, respectively). Binomial analysis of kappa values for ECT showed the superiority of indium-111 for intraobserver (p = 0.035) and interobserver (p = 0.0039) study. However, for PS there was no significant difference in reproducibility with the two radionuclides

  3. Novel Contact Materials for Improved Performance CdTe Solar Cells Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Rockett, Angus [Colorado School of Mines, Golden, CO (United States); Marsillac, Sylvain [Old Dominion Univ., Norfolk, VA (United States); Collins, Robert [Univesity of Toledo

    2018-04-15

    Te devices. We demonstrated the conduction mechanism by which CdTe polycrystals improve the performance of the devices relative to single crystal devices. The mechanism shows that grain boundaries are conduction pathways for photogenerated electrons and that the corresponding holes are confined to the grains and therefore do not contribute to recombination.

  4. Antibody biotechnology

    African Journals Online (AJOL)

    STORAGESEVER

    2009-07-06

    Jul 6, 2009 ... and automated, the hybrid cells can be stored for many years in liquid nitrogen and antibodies production is homogeneous. The hybridoma method .... they may be modified to vehicle active molecules such as radio-isotopes, toxins, cytokines, enzyme etc. In these cases, the therapeutic effect is due to ...

  5. Catalytic Antibodies

    Indian Academy of Sciences (India)

    The ability of the highly evolved machinery of immune system to produce structurally and functionally complex ... to Pauling, if the structure of the antigen binding site of antibodies were to be produced in a random ..... where the immune system of the body is destructive, as in autoimmune disorders or after organ transplant.

  6. Catalytic Antibodies

    Indian Academy of Sciences (India)

    While chemistry provides the framework for understanding the structure and function of biomolecules, the immune sys- tem provides a highly evolved natural process to generate one class of complex biomolecules – the antibodies. A combination of the two could be exploited to generate new classes of molecules with novel ...

  7. Enhanced Emission Performance and Fuel Efficiency for HD Methane Engines. Literature Study. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Broman, R.; Staalhammar, P.; Erlandsson, L.

    2010-05-15

    A literature survey has been conducted in order to define state-of-the-art for methane fuelled engines to be used in heavy duty vehicles. Use of methane can be favourable to increase security of supply and mitigate CO2 emissions, especially when the methane origins from biomass. Furthermore, methane used as a fuel in heavy duty engines has a potential to reduce toxic exhaust emissions. Historically, use of methane in heavy duty engines has often been hampered by poor efficiency, i.e. high fuel consumption when using the Otto-cycle. However, current generation technology engines might be within 5-10 % of the efficiency of Diesel engine technology. In this context it is worth mentioning that compliance-driven changes for meeting future emission regulations for Diesel engines may have a negative impact on fuel efficiency, thereby narrowing the gap. This may present an opportunity for heavy methane fuelled engines. The reliability and durability of the exhaust aftertreatment devices for methane fuelled engines has also given rise to some concerns. Some concepts are performing acceptable while others do not meet expectations. This is partly due to difficulties in handling methane in the aftertreatment device and partly to issues in the design of the ignition system. Methane is a fuel used worldwide and has a potential to be an important complement to Diesel oil. There are two categories of HD methane engines available to end-users: Retrofitted engines, which often include computer controlled retrofit systems developed as 'bolt-on' technologies that can be removed if necessary, to resell the vehicle with a normal diesel engine, and those developed specifically for and in conjunction with engine manufacturers and delivered to customers as factory-built engines or vehicles (OEM). Additionally, both these categories can include engines that use the Otto- or Diesel combustion cycles. When adapting a HD Diesel engine to run on methane there are two options, either

  8. FLOAT - development of new flexible UHPC. Final report. [Ultra High Performance Fibre Reinforced Concrete

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-11-01

    The current project is a preliminary study intended to clarify the background and give a better basis for an evaluation of the risks and possible rewards of funding a full project with the overall purpose of developing and testing a new concept for wave energy floaters, made of Ultra High Performance Fibre Reinforced Concrete (UHPC), as an enabling technology for the establishment of competitive wave energy production (FLOAT). As an initial step for this preliminary study of FLOAT an investigation has been undertaken in relation to preliminary design of 2 types of floaters, essential properties of UHPFRC - and identification of necessary developments, compilation of existing data from off shore applications and analysis of effect on Cost Of Energy. Preliminary float design and economical considerations - is a theoretical and numerical study including preliminary float designs and cost estimates. It aims at making a first comparison between the different materials options for DEXA and Wave Star floats and giving a first judgement about the suitability of CRC concrete. This is done through a qualitative assessment of pros and cons of different materials for both types of floats and a design study of the Dexa Wave float. It is concluded that the requirements for the Dexa Wave float are so that CRC is not able to compete with conventional concrete for the best and most cost effective solution. The good durability (leading to low maintenance costs), the mechanical properties and the ductility of CRC are not important enough to offset the increased cost for this float. For Wave Star on the other hand, there are significant advantages in using CRC as the only other option in this case is fibre glass, which is a much more expensive product. An investigation was made of methods of optimizing the properties of CRC - customizing them for particular applications in WEC's. The method of optimization has been to change the types of fibres in the mix, and it is demonstrated

  9. Comprehensive evaluation and optimization of amplicon library preparation methods for high-throughput antibody sequencing.

    Science.gov (United States)

    Menzel, Ulrike; Greiff, Victor; Khan, Tarik A; Haessler, Ulrike; Hellmann, Ina; Friedensohn, Simon; Cook, Skylar C; Pogson, Mark; Reddy, Sai T

    2014-01-01

    High-throughput sequencing (HTS) of antibody repertoire libraries has become a powerful tool in the field of systems immunology. However, numerous sources of bias in HTS workflows may affect the obtained antibody repertoire data. A crucial step in antibody library preparation is the addition of short platform-specific nucleotide adapter sequences. As of yet, the impact of the method of adapter addition on experimental library preparation and the resulting antibody repertoire HTS datasets has not been thoroughly investigated. Therefore, we compared three standard library preparation methods by performing Illumina HTS on antibody variable heavy genes from murine antibody-secreting cells. Clonal overlap and rank statistics demonstrated that the investigated methods produced equivalent HTS datasets. PCR-based methods were experimentally superior to ligation with respect to speed, efficiency, and practicality. Finally, using a two-step PCR based method we established a protocol for antibody repertoire library generation, beginning from inputs as low as 1 ng of total RNA. In summary, this study represents a major advance towards a standardized experimental framework for antibody HTS, thus opening up the potential for systems-based, cross-experiment meta-analyses of antibody repertoires.

  10. Detection of antibodies to co-trimoxazole (preservative drug interfering with routine red cell antibody screening

    Directory of Open Access Journals (Sweden)

    Deepti Sachan

    2018-01-01

    Full Text Available Drug-dependent antibodies can rarely cause interference in pretransfusion antibody screening. The diluents for commercial reagent red blood cells contain different antibiotics, such as chloramphenicol, neomycin sulfate, and gentamycin as a preservative. The presence of antibodies to a given drug in patient may lead to positive results when performing antibody identification. We present a rare case of detection of anti-co-trimoxazole antibody during routine antibody screening in a female patient undergoing neurosurgery. These antibodies mimicked as antibody against high-frequency red cell antigens reacting in both saline phase as well as antiglobulin phase. Anti-co-trimoxazole antibody was confirmed by repeating antibody screen using reagent red cells of different manufacturers with and without co-trimoxazole drug as preservative as well as using washed red cell panels. There were no associated clinical or laboratory evidence of hemolysis.

  11. Is performance in pre-clinical assessment a good predictor of the final Doctor of Medicine grade?

    Science.gov (United States)

    Al-Wardy, Nadia M; Rizvi, Syed G; Bayoumi, Riad A

    2009-12-01

    To investigate if any correlation exists between students' grades on their final doctor of Medicine (MD) assessment and their overall preclinical grade point average (GPA) and its component parts. Student data available from the Deanship of Admissions and Registration were analyzed. Pearson correlation coefficient was obtained to assess the degree of linear relationship between performance in the preclinical and the MD assessment of 529 students who graduated from the College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoud, Oman from June 1998 to June 2005. Simple and multiple regression analyses were performed to evaluate individual and combined impact of the preclinical courses' grades on MD grades. Preclinical GPA correlated highly with MD GPA (r=0.641). The science component taught early in the preclinical phase correlated more strongly (r=0.457) than student electives (r=0.246). This correlation was better in the good English group. Students' performance, however, was best in electives, but worst in English. Most students who had low MD GPA (2.5, and limiting the credit hour requirement of electives by the College seems to be justified.

  12. Effect of chicken egg anti-F4 antibodies on performance and diarrhea incidences in enterotoxigenic Escherichia coli K88+-challenged piglets

    Directory of Open Access Journals (Sweden)

    Kolawole Aluko

    2017-12-01

    Full Text Available The aim was to evaluate the effects of dietary supplementation of spay-dried whole egg containing anti-F4 antibodies (SDWE against recombinantly produced F4 antigens in enterotoxigenic Escherichia coli K88+ (ETEC-challenged piglets. Twenty-seven 21-d-old and individually housed piglets were randomly allotted to 3 treatments consisting of a wheat-soybean meal basal diet containing either 0 (control egg powder; CEP, 0.1% (SDWE1 or 0.4% (SDWE2 SDWE. After a 7-d adaptation period, blood samples were collected from all pigs, and pigs were weighed and orally challenged with an ETEC inoculum. Blood was sampled at 24 and 48 h post-challenge, and diarrhea incidences and scores were recorded. On d 14, all pigs were weighed and then euthanized to obtain intestinal tissue samples for histomorphology measurement. During the pre-challenge period, pigs fed the SDWE showed a linear improvement (P < 0.05 in average daily gain (ADG and gain to feed ratio (G:F, but there were no differences among treatments in growth performance during the post-challenge period. Diarrhea incidences and scores, fecal shedding of ETEC, plasma urea nitrogen content and intestinal histomorphology were similar among treatments. The results show that 0.4% SDWE supported greater piglet performance before challenge although such benefits were not evident during the post-challenge period at either 0.1% or 0.4% supplementation.

  13. [Immunohematologic study and transfusion approach to patients with public antibodies].

    Science.gov (United States)

    Solves, P; de la Rubia, J; Arriaga, F; Cervera, J; Arnao, M; Carpio, N; Marty, M L

    1997-02-01

    To analyze the different immunohematologic studies required to identify anti-red cell antibodies directed against high incidence antigens and comment the best tranfusion management. Five patients with suspected anti-red cell alloantibodies directed against high frequency antigens are reported. After a positive antibody screening test (AST), an agglutination test with a commercial panel of 24 red cells was performed. Red cells were treated with proteolytic enzymes and AET to try to identify the circulating antibody. However, it was necessary to send the samples to reference laboratories for definitive identification. In order to evaluate the haemolytic potential of the antibody serum samples were treated with DTT and immunoglobulin subtype was studied with the capillary agglutination test. Finally, we analyze the half life of Cr51 labelled red cells. To obtain compatible blood for transfusion, autologous transfusion and cross-match with blood from direct relatives were performed. AST was positive in every case. A decrease in the agglutination test was observed after ficin treatment in two patients, and an increase in the remaining. The treatment of red cells with ZZAP and AET resulted in a decrease of agglutination in three cases and an increase in the remaining two. Specificity of the antibodies was as follows: anti-Cellano (two cases), anti-Ku (one case) and anti-Yta (two cases). Anti-Kell antibodies were IgG1 and anti-Cartwright antibodies were IgG4. One patient was transfused with autologous blood alone, another patient received compatible blood from direct relatives. A third patient was transfused both with autologous and allogeneic compatible blood. The fourth patient did not need red cell transfusion and, finally the last patient had to be transfused with incompatible blood but no postransfusion haemolysis was observed. In patients with anti-red cell antibodies against high-frequency antigens, red blood cells treatment with proteolytic enzymes (ZZAP, ficin

  14. Clean Coal Technology III: 10 MW Demonstration of Gas Suspension Absorption final project performance and economics report

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, F.E.

    1995-08-01

    The 10 MW Demonstration of the Gas Suspension Absorption (GSA) program is a government and industry co-funded technology development. The objective of the project is to demonstrate the performance of the GSA system in treating a 10 MW slipstream of flue gas resulting from the combustion of a high sulfur coal. This project involves design, fabrication, construction and testing of the GSA system. The Project Performance and Economics Report provides the nonproprietary information for the ``10 MW Demonstration of the Gas Suspension Absorption (GSA) Project`` installed at Tennessee Valley Authority`s (TVA) Shawnee Power Station, Center for Emissions Research (CER) at Paducah, Kentucky. The program demonstrated that the GSA flue-gas-desulfurization (FGD) technology is capable of achieving high SO{sub 2} removal efficiencies (greater than 90%), while maintaining particulate emissions below the New Source Performance Standards (NSPS), without any negative environmental impact (section 6). A 28-day test demonstrated the reliability and operability of the GSA system during continuous operation. The test results and detailed discussions of the test data can be obtained from TVA`s Final Report (Appendix A). The Air Toxics Report (Appendix B), prepared by Energy and Environmental Research Corporation (EERC) characterizes air toxic emissions of selected hazardous air pollutants (HAP) from the GSA process. The results of this testing show that the GSA system can substantially reduce the emission of these HAP. With its lower capital costs and maintenance costs (section 7), as compared to conventional semi-dry scrubbers, the GSA technology commands a high potential for further commercialization in the United States. For detailed information refer to The Economic Evaluation Report (Appendix C) prepared by Raytheon Engineers and Constructors.

  15. Comparative Study of the Tuning Performances of the Nominal and Long L* CLIC Final Focus System at √s = 380 GeV

    CERN Document Server

    Plassard, F; Marin, E; Tomás, R

    2017-01-01

    Mitigation of static imperfections for emittance preservation is one of the most important and challenging tasks faced by the Compact Linear Collider (CLIC) beam delivery system. A simulation campaign has been performed to recover the nominal luminosity by means of different alignment procedures. The state of the art of the tuning studies is drawn up. Comparative studies of the tuning performances and a tuning-based final focus system design optimization for two L options are presented. The effectiveness of the tuning techniques applied to these different lattices will be decisive for the final layout of the CLIC final focus system at √s = 380 GeV.

  16. Performance Evaluation of Advanced Retrofit Roof Technologies Using Field-Test Data Phase Three Final Report, Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    Biswas, Kaushik [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Childs, Phillip W. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Atchley, Jerald Allen [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-01-01

    This article presents some miscellaneous data from two low-slope and two steep-slope experimental roofs. The low-slope roofs were designed to compare the performance of various roof coatings exposed to natural weatherization. The steep-slope roofs contained different combinations of phase change material, rigid insulation, low emittance surface and above-sheathing ventilation, with standing-seam metal panels on top. The steep-slope roofs were constructed on a series of adjacent attics separated at the gables using thick foam insulation. This article describes phase three (3) of a study that began in 2009 to evaluate the energy benefits of a sustainable re-roofing technology utilizing standing-seam metal roofing panels combined with energy efficient features like above-sheathing-ventilation (ASV), phase change material (PCM) and rigid insulation board. The data from phases 1 and 2 have been previously published and reported [Kosny et al., 2011; Biswas et al., 2011; Biswas and Childs, 2012; Kosny et al., 2012]. Based on previous data analyses and discussions within the research group, additional test roofs were installed in May 2012, to test new configurations and further investigate different components of the dynamic insulation systems. Some experimental data from phase 3 testing from May 2012 to December 2013 and some EnergyPlus modeling results have been reported in volumes 1 and 3, respectively, of the final report [Biswas et al., 2014; Biswas and Bhandari, 2014].

  17. C-Band Airport Surface Communications System Standards Development. Phase II Final Report. Volume 2: Test Bed Performance Evaluation and Final AeroMACS Recommendations

    Science.gov (United States)

    Hall, Edward; Magner, James

    2011-01-01

    This report is provided as part of ITT s NASA Glenn Research Center Aerospace Communication Systems Technical Support (ACSTS) contract NNC05CA85C, Task 7: New ATM Requirements-Future Communications, C-Band and L-Band Communications Standard Development and was based on direction provided by FAA project-level agreements for New ATM Requirements-Future Communications. Task 7 included two subtasks. Subtask 7-1 addressed C-band (5091- to 5150-MHz) airport surface data communications standards development, systems engineering, test bed and prototype development, and tests and demonstrations to establish operational capability for the Aeronautical Mobile Airport Communications System (AeroMACS). Subtask 7-2 focused on systems engineering and development support of the L-band digital aeronautical communications system (L-DACS). Subtask 7-1 consisted of two phases. Phase I included development of AeroMACS concepts of use, requirements, architecture, and initial high-level safety risk assessment. Phase II builds on Phase I results and is presented in two volumes. Volume I is devoted to concepts of use, system requirements, and architecture, including AeroMACS design considerations. Volume II (this document) describes an AeroMACS prototype evaluation and presents final AeroMACS recommendations. This report also describes airport categorization and channelization methodologies. The purposes of the airport categorization task were (1) to facilitate initial AeroMACS architecture designs and enable budgetary projections by creating a set of airport categories based on common airport characteristics and design objectives, and (2) to offer high-level guidance to potential AeroMACS technology and policy development sponsors and service providers. A channelization plan methodology was developed because a common global methodology is needed to assure seamless interoperability among diverse AeroMACS services potentially supplied by multiple service providers.

  18. In depth evaluation of the prognostic and predictive utility of PTEN immunohistochemistry in colorectal carcinomas: performance of three antibodies with emphasis on intracellular and intratumoral heterogeneity.

    Science.gov (United States)

    Ágoston, Emese Irma; Micsik, Tamás; Ács, Balázs; Fekete, Krisztina; Hahn, Oszkár; Baranyai, Zsolt; Dede, Kristóf; Bodoky, György; Bursics, Attila; Kulka, Janina; Krenács, Tibor; Győrffy, Balázs; Harsányi, László; Szász, A Marcell

    2016-07-08

    Phosphatase and tensin homolog deleted in chromosome 10 (PTEN) loss of function is frequently detected in advanced colorectal cancer. Its detection is thought to have prognostic significance and it is being considered to predict responsiveness to anti-EGFR therapy. Unfortunately, while immunohistochemical assessment of PTEN expression is widespread, it lacks standardization and the results are hardly comparable across the available publications. Retrospectively collected, formalin-fixed and paraffin-embedded colorectal tumor tissue samples from 55 patients were combined into tissue microarray (TMA) blocks. We used three different PTEN antibodies to determine the frequency, intensity and intracellular pattern of PTEN immunohistochemical labeling: Neomarkers, Dako and CellSignaling. We evaluated the aforementioned parameters in selected regions of colorectal cancers and in their lymph node metastases by using three scoring methods that take into consideration both staining frequency and intensity (H1-H3-score). We also evaluated intracellular localization. The Dako and CellSignaling antibodies stained predominantly cytoplasms, while the Neomarkers antibody specifically stained cell nuclei. PTEN H-scores were significantly lower in all tumor areas as compared to the normal colonic mucosa based on staining with the DAKO and CellSignaling antibodies. Intratumoral regional differences or differences between matching tumors and metastases were not detected with any of the antibodies. Neither Dako, neither CellSignaling, nor the Neomarkers antibodies revealed a significant correlation between PTEN expression and pT, Dukes/MAC and clinical stage. KRAS status, histological grade correlated with PTEN H-scores based on staining with the Neomarkers antibody. PTEN H-scores did not correlate with MMR status. PTEN H-scores did not show any correlation with relapse-free survival based on staining with either antibody. While PTEN expression decreased in colorectal cancer according

  19. Blinn College Final Grade Distribution Report for Spring 1994 Semester. Student Performance Report. International Research Document No. 012E.

    Science.gov (United States)

    Blinn Coll., Brenham, TX.

    Blinn College final course grade distributions are summarized for spring 1990 to 1994 in this four-part report. Section I presents tables of final grade distributions by campus and course in accounting; agriculture; anthropology; biology; business; chemistry; child development; communications; computer science; criminal justice; drama; emergency…

  20. [Radiolabeled antibodies for cancer treatment].

    Science.gov (United States)

    Barbet, Jacques; Chatal, Jean-François; Kraeber-Bodéré, Françoise

    2009-12-01

    The first treatment ever by radio-immunotherapy (RIT) was performed by William H. Beierwaltes in 1951 and was a success. Fifty years later, the main question is to find ways of extending the success of radiolabelled anti-CD20 antibodies in indolent non-Hodgkin's lymphoma to other forms of cancer. Solid tumours are much more radioresistant than lymphomas, but they respond to RIT if the lesions are small. Clinical situations of residual or minimal disease are thus the most likely to benefit from RIT in the adjuvant or consolidation settings. For disseminated disease, like leukemias or myelomas, the problem is different: beta- particles emitted by the radioactive atoms classically used for cancer treatment (iodine-131 or yttrium-90) disperse their energy in large volumes (ranges 1 mm to 1 cm) and are not very effective against isolated cells. Advances in RIT progress in two directions. One is the development of pretargeting strategies in which the antibody is not labelled but used to provide binding sites to small molecular weight radioactivity vectors (biotin, haptens). These techniques have been shown to increase tumour to non-target uptake ratios and anti-tumour efficacy has been demonstrated in the clinic. The other approach is the use of radionuclides adapted to the various clinical situations. Lutetium-177 or copper-67, because of the lower energy of their emission, their relatively long half-life and good gamma emission, may significantly improve RIT efficacy and acceptability. Beyond that, radionuclides emitting particles such as alpha particles or Auger electrons, much more efficient to kill isolated tumour cells, are being tested for RIT in the clinic. Finally, RIT should be integrated with other cancer treatment approaches in multimodality protocols. Thus RIT, now a mature technology, should enter a phase of well designed and focused clinical developments that may be expected to afford significant therapeutic advances.

  1. Magnetic Purification of Antibodies

    Science.gov (United States)

    Dhadge, Vijaykumar Laxman

    This work aimed at the development of magnetic nanoparticles for antibody purification and at the evaluation of their performance in Magnetic fishing and in a newly developed hybrid technology Magnetic Aqueous Two Phase Systems. Magnetic materials were produced by coprecipitation and solvothermal approaches. Natural polymers such as dextran, extracellular polysaccharide and gum Arabic were employed for coating of iron oxide magnetic supports. Polymer coated magnetic supports were then modified with synthetic antibody specific ligands,namely boronic acid, a triazine ligand (named 22/8) and an Ugi ligand (named A2C7I1). To optimize the efficacy of magnetic nanoparticles for antibody magnetic fishing, various solutions of pure and crude antibody solutions along with BSA as a non-specific binding protein were tested. The selectivity of magnetic nanoparticle for antibody, IgG, was found effective with boronic acid and ligand 22/8. Magnetic supports were then studied for their performance in high gradient magnetic separator for effective separation capability as well as higher volume handling capability. The magnetic materials were also supplemented to aqueous two phase systems, devising a new purification technology. For this purpose, magnetic particles modified with boronic acid were more effective. This alternative strategy reduced the time of operation,maximized separation capability (yield and purity), while reducing the amount of salt required. Boronic acid coated magnetic particles bound 170 +/- 10 mg hIgG/g MP and eluted 160 +/- 5 mg hIgG/g MP, while binding only 15 +/- 5 mg BSA/g MP. The affinity constant for the interaction between hIgG and APBA_MP was estimated as 4.9 x 105 M-1 (Ka) with a theoretical maximum capacity of 492 mg hIgG adsorbed/g MP (Qmax). APBA_MPs were also tested for antibody purification directly from CHO cell supernatants. The particles were able to bind 98% of IgG loaded and to recover 95% of pure IgG (purity greater than 98%) at extremely

  2. Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification [version 2; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Marco-Antonio Mendoza-Parra

    2016-03-01

    Full Text Available We have established a certification system for antibodies to be used in chromatin immunoprecipitation assays coupled to massive parallel sequencing (ChIP-seq. This certification comprises a standardized ChIP procedure and the attribution of a numerical quality control indicator (QCi to biological replicate experiments. The QCi computation is based on a universally applicable quality assessment that quantitates the global deviation of randomly sampled subsets of ChIP-seq dataset with the original genome-aligned sequence reads. Comparison with a QCi database for >28,000 ChIP-seq assays were used to attribute quality grades (ranging from ‘AAA’ to ‘DDD’ to a given dataset. In the present report we used the numerical QC system to assess the factors influencing the quality of ChIP-seq assays, including the nature of the target, the sequencing depth and the commercial source of the antibody.  We have used this approach specifically to certify mono and polyclonal antibodies obtained from Active Motif directed against the histone modification marks H3K4me3, H3K27ac and H3K9ac for ChIP-seq. The antibodies received the grades AAA to BBC (www.ngs-qc.org. We propose to attribute such quantitative grading of all antibodies attributed with the label “ChIP-seq grade”.

  3. Final Report

    DEFF Research Database (Denmark)

    Heiselberg, Per; Brohus, Henrik; Nielsen, Peter V.

    This final report for the Hybrid Ventilation Centre at Aalborg University describes the activities and research achievement in the project period from August 2001 to August 2006. The report summarises the work performed and the results achieved with reference to articles and reports published...

  4. Operation and Performance of a Biphase Turbine Power Plant at the Cerro Prieto Geothermal Field (Final Report)

    Energy Technology Data Exchange (ETDEWEB)

    Hays, Lance G. [Douglas Energy Company, Placentia, CA (United States)

    2000-09-01

    A full scale, wellhead Biphase turbine was manufactured and installed with the balance of plant at Well 103 of the Cerro Prieto geothermal resource in Baja, California. The Biphase turbine was first synchronized with the electrical grid of Comision Federal de Electricidad on August 20, 1997. The Biphase power plant was operated from that time until May 23, 2000, a period of 2 years and 9 months. A total of 77,549 kWh were delivered to the grid. The power plant was subsequently placed in a standby condition pending replacement of the rotor with a newly designed, higher power rotor and replacement of the bearings and seals. The maximum measured power output of the Biphase turbine, 808 kWe at 640 psig wellhead pressure, agreed closely with the predicted output, 840 kWe. When combined with the backpressure steam turbine the total output power from that flow would be increased by 40% above the power derived only from the flow by the present flash steam plant. The design relations used to predict performance and design the turbine were verified by these tests. The performance and durability of the Biphase turbine support the conclusion of the Economics and Application Report previously published, (Appendix A). The newly designed rotor (the Dual Pressure Rotor) was analyzed for the above power condition. The Dual Pressure Rotor would increase the power output to 2064 kWe by incorporating two pressure letdown stages in the Biphase rotor, eliminating the requirement for a backpressure steam turbine. The power plant availability was low due to deposition of solids from the well on the Biphase rotor and balance of plant problems. A great deal of plant down time resulted from the requirement to develop methods to handle the solids and from testing the apparatus in the Biphase turbine. Finally an online, washing method using the high pressure two-phase flow was developed which completely eliminated the solids problem. The availability of the Biphase turbine itself was 100

  5. Duration of antibody response following vaccination against feline immunodeficiency virus.

    Science.gov (United States)

    Westman, Mark E; Malik, Richard; Hall, Evelyn; Harris, Matthew; Hosie, Margaret J; Norris, Jacqueline M

    2017-10-01

    most cats (10/12) by 1 month after the third (final) primary FIV vaccination. All cats tested negative using Witness and Anigen Rapid 6 months after the third primary FIV vaccination. Conclusions and relevance This study has shown that a primary course of FIV vaccination does not interfere with FIV antibody testing in cats using Witness and Anigen Rapid, provided primary vaccination has not occurred within the previous 6 months. Consequently, Witness and Anigen Rapid antibody test kits can be used reliably to determine FIV infection status at the time of annual booster FIV vaccination to help detect 'vaccine breakthroughs' and in cats that have not received a primary course of FIV vaccination within the preceding 6 months. The duration of antibody response following annual booster FIV vaccination and the resulting effect on antibody testing using PoC kits needs to be determined by further research. The mechanism(s) for the variation in FIV antibody test kit performance remains unclear.

  6. Enhanced performance of an innovative dengue IgG/IgM rapid diagnostic test using an anti-dengue EDI monoclonal antibody and dengue virus antigen

    Science.gov (United States)

    Lee, Jihoo; Kim, Young-Eun; Kim, Hak-Yong; Sinniah, Mangalam; Chong, Chom-Kyu; Song, Hyun-Ok

    2015-01-01

    High levels of anti-dengue IgM or IgG can be detected using numerous rapid diagnostic tests (RDTs). However, the sensitivity and specificity of these tests are reduced by changes in envelope glycoprotein antigenicity that inevitably occur in limited expression systems. A novel RDT was designed to enhance diagnostic sensitivity. Dengue viruses cultured in animal cells were used as antigens to retain the native viral coat protein. Monoclonal antibodies (mAbs) were then developed, for the first time, against domain I of envelope glycoprotein (EDI). The anti-dengue EDI mAb was employed as a capturer, and EDII and EDIII, which are mainly involved in the induction of neutralizing antibodies in patients, were fully available to bind to anti-dengue IgM or IgG in patients. A one-way automatic blood separation device prevented reverse migration of plasma and maximize the capture of anti-dengue antibodies at the test lines. A clinical evaluation in the field proved that the novel RDT (sensitivities of 96.5% and 96.7% for anti-dengue IgM and IgG) is more effective in detecting anti-dengue antibodies than two major commercial tests (sensitivities of 54.8% and 82% for SD BIOLINE; 50.4% and 75.3% for PanBio). The innovative format of RDT can be applied to other infectious viral diseases. PMID:26655854

  7. Antibodies: an alternative for antibiotics?

    Science.gov (United States)

    Berghman, L R; Abi-Ghanem, D; Waghela, S D; Ricke, S C

    2005-04-01

    In 1967, the success of vaccination programs, combined with the seemingly unstoppable triumph of antibiotics, prompted the US Surgeon General to declare that "it was time to close the books on infectious diseases." We now know that the prediction was overly optimistic and that the fight against infectious diseases is here to stay. During the last 20 yr, infectious diseases have indeed made a staggering comeback for a variety of reasons, including resistance against existing antibiotics. As a consequence, several alternatives to antibiotics are currently being considered or reconsidered. Passive immunization (i.e., the administration of more or less pathogen-specific antibodies to the patient) prior to or after exposure to the disease-causing agent is one of those alternative strategies that was almost entirely abandoned with the introduction of chemical antibiotics but that is now gaining interest again. This review will discuss the early successes and limitations of passive immunization, formerly referred to as "serum therapy," the current use of antibody administration for prophylaxis or treatment of infectious diseases in agriculture, and, finally, recent developments in the field of antibody engineering and "molecular farming" of antibodies in various expression systems. Especially the potential of producing therapeutic antibodies in crops that are routine dietary components of farm animals, such as corn and soy beans, seems to hold promise for future application in the fight against infectious diseases.

  8. Dietary supplementation of organic selenium could improve performance, antibody response, and yolk oxidative stability in laying hens fed on diets containing oxidized fat.

    Science.gov (United States)

    Laika, M; Jahanian, R

    2015-06-01

    The present study was carried out to investigate the effect of dietary supplementation of organic selenium (Se) on performance, egg quality indices, and yolk oxidative stability in laying hens fed diets with different fat sources. A total of 270 Hy-line W-36 Leghorn hens of 47 weeks of age were randomly distributed into the 5 replicate cages of 9 dietary treatments. Experimental diets consisted of a 3 × 3 factorial arrangement of treatments with three different fat sources (soybean oil, SO; yellow grease, YG; and palm fat powder, PFP) and three different levels of supplemental Se (0, 0.2, and 0.4 mg/kg of diet) as supplied by zinc-L-selenomethionine (ZnSeMet) complex, which fed during a 77-day feeding trial including 7 days for adaptation and 70 days as the main recording period. Results showed that the highest (P hens fed on SO-supplemented diets. Hen-day egg production was affected by both dietary fat source (P feed intake was not affected by experimental diets during the first 35-day period, dietary inclusion of PFP reduced feed intake during both second 35-day (P feed conversion ratio during the first 35-day period was assigned to the birds fed on SO-diets, followed by those fed YG-diets. Dietary supplementation of ZnSeMet improved (P feed efficiency during the first 35-day period. Supplementation of ZnSeMet into the diets increased yolk index, with more impact in hens fed on YG-diets. The highest concentration of yolk malondialdehyde was observed in YG-fed groups, and ZnSeMet supplementation of diets decreased (P hens fed on diets supplemented by YG, followed by those on SO-diets. Although different fat sources had no effect on antibody titer against Newcastle disease virus, supplemental ZnSeMet improved (P hens, with the highest impact in diets containing oxidized (high peroxide values) fat sources.

  9. Services for Deaf Blind Children and Youth. Final Performance Report, 10/01/89-9/30/92.

    Science.gov (United States)

    Nezol, A. James

    This final report describes a 3-year project to provide services to all children (approximately 144) between 0-21 years with deaf blindness in Colorado. The project addressed seven areas: (1) information for identification, (2) technical assistance to develop services, (3) home-based services to infants and toddlers, (4) transition services, (5)…

  10. Clinical utility of anti-p53 auto-antibody: systematic review and focus on colorectal cancer.

    Science.gov (United States)

    Suppiah, Aravind; Greenman, John

    2013-08-07

    Mutation of the p53 gene is a key event in the carcinogenesis of many different types of tumours. These can occur throughout the length of the p53 gene. Anti-p53 auto-antibodies are commonly produced in response to these p53 mutations. This review firstly describes the various mechanisms of p53 dysfunction and their association with subsequent carcinogenesis. Following this, the mechanisms of induction of anti-p53 auto-antibody production are shown, with various hypotheses for the discrepancies between the presence of p53 mutation and the presence/absence of anti-p53 auto-antibodies. A systematic review was performed with a descriptive summary of key findings of each anti-p53 auto-antibody study in all cancers published in the last 30 years. Using this, the cumulative frequency of anti-p53 auto-antibody in each cancer type is calculated and then compared with the incidence of p53 mutation in each cancer to provide the largest sample calculation and correlation between mutation and anti-p53 auto-antibody published to date. Finally, the review focuses on the data of anti-p53 auto-antibody in colorectal cancer studies, and discusses future strategies including the potentially promising role using anti-p53 auto-antibody presence in screening and surveillance.

  11. Integrated Advanced Microwave Sounding Unit-A (AMSU-A). Performance Verification Report: Final Comprehensive Performance Test Report, P/N 1331720-2TST, S/N 105/A1

    Science.gov (United States)

    Platt, R.

    1999-01-01

    This is the Performance Verification Report, Final Comprehensive Performance Test (CPT) Report, for the Integrated Advanced Microwave Sounding Unit-A (AMSU-A). This specification establishes the requirements for the CPT and Limited Performance Test (LPT) of the AMSU-1A, referred to here in as the unit. The sequence in which the several phases of this test procedure shall take place is shown.

  12. Medicare Program; Medicare Shared Savings Program; Accountable Care Organizations--Revised Benchmark Rebasing Methodology, Facilitating Transition to Performance-Based Risk, and Administrative Finality of Financial Calculations. Final rule.

    Science.gov (United States)

    2016-06-10

    Under the Medicare Shared Savings Program (Shared Savings Program), providers of services and suppliers that participate in an Accountable Care Organization (ACO) continue to receive traditional Medicare fee-for-service (FFS) payments under Parts A and B, but the ACO may be eligible to receive a shared savings payment if it meets specified quality and savings requirements. This final rule addresses changes to the Shared Savings Program, including: Modifications to the program's benchmarking methodology, when resetting (rebasing) the ACO's benchmark for a second or subsequent agreement period, to encourage ACOs' continued investment in care coordination and quality improvement; an alternative participation option to encourage ACOs to enter performance-based risk arrangements earlier in their participation under the program; and policies for reopening of payment determinations to make corrections after financial calculations have been performed and ACO shared savings and shared losses for a performance year have been determined.

  13. Monoclonal antibodies technology. Protocols

    International Nuclear Information System (INIS)

    Acevado Castro, B.E.

    1997-01-01

    Full text: Immunization. The first step in preparing useful monoclonal antibodies (MAbs) is to immunize an animal (Balb/c for example) with an appropriate antigen. Methods (only for soluble antigen): Solubilize selected antigen in Phosphate buffer solution (PBS) at pH 7.2-7.4, ideally at a final concentration per animal between 10 to 50 μg/ml. It is recommended that the antigen under consideration be incorporated into the emulsion adjuvants in 1:1 volumetric relation. We commonly use Frend's adjuvant (FA) to prepared immunized solution. The first immunization should be prepared with complete FA, and the another could be prepared with incomplete FA. It is recommended to inject mice with 0.2 ml intraperitoneal (ip) or subcutaneous (sc). Our experience suggests the sc route is the preferred route. A minimum protocol for immunizing mice to generate cells for preparing hybridomas is s follows: immunize sc on day 0, boost sc on day 21, take a trial bleeding on day 26; if antibody titters are satisfactory, boost ip on day 35 with antigen only, and remove the spleen to obtain cells for fusion on day 38. Fusion protocol. The myeloma cell line we are using is X63 Ag8.653. At the moment of fusion myeloma cells need a good viability (at least a 95%). 1. Remove the spleen cells from immunized mice using sterile conditions. An immune spleen should yield between 7 a 10x10 7 nucleated cells. 2. Place the spleen in 20 ml of serum-free RPMI 1640 in a Petri dish. Using a needle and syringe, inject the spleen with medium to distend and disrupt the spleen stroma and free the nucleated cells. 3. Flush the cell suspension with a Pasteur pipet to disperse clumps of cells. 4. Centrifuge the spleen cell suspension at 250g for 10 min. Resuspend the pellet in serum-free RPMI 1640. Determine cell concentration using Neuhabuer chamber. 5. Mix the myeloma cells and spleen cells in a conical 50-ml tube in serum-free RPMI 1640, 1 x10 7 spleen cells to 1x10 6 myeloma cells (ratio 10:1). Centrifuge

  14. Inclusion of anti-phospholipase A2 antibody to backgrounding diets on performance, feed efficiency, in vitro fermentation, and the acute-phase response of growing beef calves.

    Science.gov (United States)

    Mercadante, V R G; Waters, K M; Marquezini, G H L; Henry, D D; Ciriaco, F M; Arthington, J D; DiLorenzo, N; Lamb, G C

    2015-01-01

    In Exp. 1, individual performance and daily DMI was measured on 70 crossbred weaned calves during a 70-d period using a GrowSafe system (GrowSafe Systems Ltd., Airdrie, AB, Canada) at the University of Florida North Florida Research and Education Center Feed Efficiency Facility (FEF). Calves were fed a low-concentrate (LC) growing diet, blocked by weight and sex, and then randomly assigned to pens to receive either no additional supplement (CON; n = 35) or receive a supplement of anti-phospholipase A2 antibody (aPLA2) at an inclusion rate of 0.6% of the diet DM (n = 35). After the 70-d feed efficiency (FE) trial (Phase 1), calves were loaded into a commercial livestock trailer and were driven for approximately 1,600 km during 24 h. Upon return to the FEF (Phase 2), calves were relocated to the same pens and groups and received the same diets and treatments for 28 d. Blood samples from each calf were collected on d 0, 1, 3, 5, 7, 14, 21, and 28 relative to initiation of transportation and were analyzed for determination of concentrations of plasma ceruloplasmin and haptoglobin. In Phase 1, initial BW (242.0 ± 3.7 kg; P = 0.92), BW at d 70 (313.0 ± 4.1 kg; P = 0.79), and ADG (1.01 ± 0.02 kg; P = 0.95) were similar between treatments. However, daily DMI was greater (P = 0.01) for CON (9.18 ± 0.15 kg) than aPLA2 (8.53 ± 0.15 kg). In addition, residual feed intake was greater (P = 0.002) for CON (0.389 ± 0.110 kg/d) than aPLA2 calves (-0.272 ± 0.110 kg/d). In Phase 2, after transportation, there were no differences between treatments on BW loss due to transportation shrink (26.0 ± 0.6 kg; P = 0.86), BW at d 28 (339.0 ± 4.1 kg; P = 0.72), ADG (1.28 ± 0.03 kg/d; P = 0.72), G:F (0.164 ± 0.004; P = 0.83), and concentrations of plasma haptoglobin (0.08 ± 0.02 mg/mL; P = 0.41). However, concentrations of plasma ceruloplasmin were greater (P calves (14.3 ± 0.3 mg/dL) compared to aPLA2 calves (13.0 ± 0.3 mg/dL). In Exp. 2, the effects of aPLA2 inclusion on LC and

  15. Remuneration of authors and performers for the use of their works and the fixation of their performancess. - Final report

    NARCIS (Netherlands)

    Guibault, L.; Salamanca, O.; van Gompel, S.

    2015-01-01

    This study analyses the current situation regarding the level of remuneration paid to authors and performers in the music and audio-visual sectors. We compare, from both a legal and economic perspective, the existing national systems of remuneration for authors and performers and identify the

  16. Arts and Entertainment Career Conference. Walt Disney Studios. Final Project Performance Report, July 31, 1978-July 31, 1979.

    Science.gov (United States)

    Walt Disney Productions, Anaheim, CA.

    The intention of a project was (1) to encourage college and university deans and heads of performing arts departments to hold an Arts and Entertainment Career Seminar on their own compus for faculty and performing arts majors and (2) to provide these institutions with written and visual materials for such a seminar. Two conferences were held, one…

  17. A Big Apple for Educators: New York City's Experiment with Schoolwide Performance Bonuses. Final Evaluation Report. Monograph

    Science.gov (United States)

    Marsh, Julie A.; Springer, Matthew G.; McCaffrey, Daniel F.; Yuan, Kun; Epstein, Scott; Koppich, Julia; Kalra, Nidhi; DiMartino, Catherine; Peng, Art

    2011-01-01

    In the 2007-2008 school year, the New York City Department of Education and the United Federation of Teachers jointly implemented the Schoolwide Performance Bonus Program in a random sample of the city's high-needs public schools. The program lasted for three school years, and its broad objective was to improve student performance through…

  18. Radiolabeled antibodies for cancer imaging and therapy.

    Science.gov (United States)

    Barbet, Jacques; Bardiès, Manuel; Bourgeois, Mickael; Chatal, Jean-François; Chérel, Michel; Davodeau, François; Faivre-Chauvet, Alain; Gestin, Jean-François; Kraeber-Bodéré, Françoise

    2012-01-01

    Radiolabeled antibodies were studied first for tumor detection by single-photon imaging, but FDG PET stopped these developments. In the meantime, radiolabeled antibodies were shown to be effective in the treatment of lymphoma. Radiolabeling techniques are well established and radiolabeled antibodies are a clinical and commercial reality that deserves further studies to advance their application in earlier phase of the diseases and to test combination and adjuvant therapies including radiolabeled antibodies in hematological diseases. In solid tumors, more resistant to radiations and less accessible to large molecules such as antibodies, clinical efficacy remains limited. However, radiolabeled antibodies used in minimal or small-size metastatic disease have shown promising clinical efficacy. In the adjuvant setting, ongoing clinical trials show impressive increase in survival in otherwise unmanageable tumors. New technologies are being developed over the years: recombinant antibodies and pretargeting approaches have shown potential in increasing the therapeutic index of radiolabeled antibodies. In several cases, clinical trials have confirmed preclinical studies. Finally, new radionuclides, such as lutetium-177, with better physical properties will further improve the safety of radioimmunotherapy. Alpha particle and Auger electron emitters offer the theoretical possibility to kill isolated tumor cells and microscopic clusters of tumor cells, opening the perspective of killing the last tumor cell, which is the ultimate challenge in cancer therapy. Preliminary preclinical and preliminary clinical results confirm the feasibility of this approach.

  19. Acetylcholine receptor antibody

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/003576.htm Acetylcholine receptor antibody To use the sharing features on this page, please enable JavaScript. Acetylcholine receptor antibody is a protein found in the blood ...

  20. Platelet antibodies blood test

    Science.gov (United States)

    This blood test shows if you have antibodies against platelets in your blood. Platelets are a part of the blood ... Chernecky CC, Berger BJ. Platelet antibody - blood. In: Chernecky ... caused by platelet destruction, hypersplenism, or hemodilution. ...

  1. European contribution to heat exchanger design/performance: HTFS research and computer programs. Final report. [SCON4 and CEMP1

    Energy Technology Data Exchange (ETDEWEB)

    Lang, C.; Skrovanek, T.

    1979-01-01

    The performance of selected HTFS computer program in solving example heat exchanger design/performance situations is evaluated. In addition, the specific inputs required by the program are presented to give an idea of the amount of detail the user must supply concerning the heat transfer system. Comments on the versatility of each program and technical details necessary for running each program are also provided. The computer programs examined rate the performance of shell-and-tube type condensers and plate-fin heat exchangers.

  2. Evaluation and Development of Pavement Scores, Performance Models and Needs Estimates for the TXDOT Pavement Management Information System : Final Report

    Science.gov (United States)

    2012-10-01

    This project conducted a thorough review of the existing Pavement Management Information System (PMIS) database, : performance models, needs estimates, utility curves, and scores calculations, as well as a review of District practices : concerning th...

  3. Snakes antibodies.

    Science.gov (United States)

    Gambón-Deza, Francisco; Sánchez-Espinel, Christian; Mirete-Bachiller, Serafín; Magadán-Mompó, Susana

    2012-09-01

    Immunoglobulins are basic molecules of the immune system of vertebrates. In previous studies we described the immunoglobulins found in two squamata reptiles, Anolis carolinensis and Eublepharis macularius. Snakes are squamata reptiles too but they have undergone an extreme evolutionary process. We therefore wanted to know how these changes affected their immunoglobulin coding genes. To perform this analysis we studied five snake transcriptomes and two genome draft sequences. Sequences coding for immunoglobulin M (IgM), immunoglobulin D (IgD) and two classes of immunoglobulin Y (IgY - named IgYa and IgYb-) were found in all of them. Moreover, the Thamnophis elegans transcriptome and Python molurus genome draft sequences showed a third class of IgY, the IgYc, whose constant region only presents three domains and lacks the CH2. All data suggest that the IgYb is the evolutionary origin of this IgYc. An exhaustive search of the light chains were carried out, being lambda the only light chain found in snakes. The results provide a clear picture of the immunoglobulins present in the suborder Serpentes. Copyright © 2012 Elsevier Ltd. All rights reserved.

  4. Novel Power Electronics Systems for Wind Energy Applications: Final Report; Period of Performance: August 24, 1999 -- November 30, 2002

    Energy Technology Data Exchange (ETDEWEB)

    Erickson, R.; Angkititrakul, S.; Al-Naseem, O.; Lujan, G.

    2004-10-01

    The objective of this work was to develop new approaches to the power electronics of variable-speed wind power systems, with the goal of improving the associated cost of energy. Of particular importance is the converter efficiency at low-wind operating points. Developing converter approaches that maintain high efficiency at partial power, without sacrificing performance at maximum power, is desirable, as is demonstrating an approach that can use emerging power component technologies to attain these performance goals with low projected capital costs. In this report, we show that multilevel conversion is an approach that can meet these performance requirements. In the wind power application, multilevel conversion proves superior to conventional converter technologies because it is callable to high power and higher voltage levels, it extends the range of high converter efficiency to lower wind speeds, and it allows superior low-voltage fast-switching semiconductor devices to be used in high-voltage high-power applications.

  5. Clusters of Tasks Performed by Merchandising Employees Working in Three Standard Industrial Classifications of Retail Establishments. Final Report, No. 20.

    Science.gov (United States)

    Ertel, Kenneth Arthur

    The study assembled up-to-date facts by questionnaire about major types of tasks actually performed by merchandising employees working in department, variety, and general merchandise stores in King and Pierce Counties, Washington. This population closely matched the national percentages, and responses of 609 employees provided data for the study.…

  6. A Performance-Oriented Electronics Technician Training Program. V. Final Fleet Follow-Up Evaluation of Graduates.

    Science.gov (United States)

    Van Matre, Nicholas H.; Harrigan, Robert J.

    An investigation was made of the job proficiency of the graduates of an experimental job-oriented training program for Electronics Technician (X-ET). This program was designed to train lower-aptitude personnel in a relatively shorter time to assume ET duties in the fleet. The fleet performance capabilities of 51 X-ET's and a matched sample of 43…

  7. High Performance Packaging Solutions for Low Cost, Reliable PV Modules: Final Subcontract Report, 26 May 2005 - 30 November 2008

    Energy Technology Data Exchange (ETDEWEB)

    Keotla, B. M.; Marinik, B. J.

    2009-06-01

    During this research effort, Dow Corning Corporation has addressed the PV manufacturing goals of: (i) improving PV manufacturing processes and equipment; (ii) accelerating manufacturing cost reductions of PV modules; (iii) increasing commercial product performance and reliability; and (iv) scaling up U.S. manufacturing capacity.

  8. Radiolabeled antibody imaging

    International Nuclear Information System (INIS)

    Wahl, R.L.

    1987-01-01

    Radiolabeled antibodies, in particular monoclonal antibodies, offer the potential for the specific nuclear imaging of malignant and benign diseases in man. If this imaging potential is realized, they may also have a large role in cancer treatment. This paper reviews: (1) what monoclonal antibodies are and how they differ from polyclonal antibodies, (2) how they are produced and radiolabeled, (3) the results of preclinical and clinical trials in cancer imaging, including the utility of SPECT and antibody fragments, (4) the role of antibodies in the diagnosis of benign diseases, (5) alternate routes of antibody delivery, (6) the role of these agents in therapy, and (7) whether this technology ''revolutionizes'' the practice of nuclear radiology, or has a more limited complementary role in the imaging department

  9. Final LDRD report : science-based solutions to achieve high-performance deep-UV laser diodes.

    Energy Technology Data Exchange (ETDEWEB)

    Armstrong, Andrew M.; Miller, Mary A.; Crawford, Mary Hagerott; Alessi, Leonard J.; Smith, Michael L.; Henry, Tanya A.; Westlake, Karl R.; Cross, Karen Charlene; Allerman, Andrew Alan; Lee, Stephen Roger

    2011-12-01

    We present the results of a three year LDRD project that has focused on overcoming major materials roadblocks to achieving AlGaN-based deep-UV laser diodes. We describe our growth approach to achieving AlGaN templates with greater than ten times reduction of threading dislocations which resulted in greater than seven times enhancement of AlGaN quantum well photoluminescence and 15 times increase in electroluminescence from LED test structures. We describe the application of deep-level optical spectroscopy to AlGaN epilayers to quantify deep level energies and densities and further correlate defect properties with AlGaN luminescence efficiency. We further review our development of p-type short period superlattice structures as an approach to mitigate the high acceptor activation energies in AlGaN alloys. Finally, we describe our laser diode fabrication process, highlighting the development of highly vertical and smooth etched laser facets, as well as characterization of resulting laser heterostructures.

  10. Fort Hood Solar Total Energy Project. Volume II. Preliminary design. Part 2. System performance and supporting studies. Final report

    Energy Technology Data Exchange (ETDEWEB)

    None,

    1979-01-01

    The preliminary design developed for the Solar Total Energy System to be installed at Fort Hood, Texas, is presented. System performance analysis and evaluation are described. Feedback of completed performance analyses on current system design and operating philosophy is discussed. The basic computer simulation techniques and assumptions are described and the resulting energy displacement analysis is presented. Supporting technical studies are presented. These include health and safety and reliability assessments; solar collector component evaluation; weather analysis; and a review of selected trade studies which address significant design alternatives. Additional supporting studies which are generally specific to the installation site are reported. These include solar availability analysis; energy load measurements; environmental impact assessment; life cycle cost and economic analysis; heat transfer fluid testing; meteorological/solar station planning; and information dissemination. (WHK)

  11. Individual plant examination program: Perspectives on reactor safety and plant performance. Part 1: Final summary report; Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-01

    This report provides perspectives gained by reviewing 75 Individual Plant Examination (IPE) submittals pertaining to 108 nuclear power plant units. IPEs are probabilistic analyses that estimate the core damage frequency (CDF) and containment performance for accidents initiated by internal events. The US Nuclear Regulatory Commission (NRC) reviewed the IPE submittals with the objective of gaining perspectives in three major areas: (1) improvements made to individual plants as a result of their IPEs and the collective results of the IPE program, (2) plant-specific design and operational features and modeling assumptions that significantly affect the estimates of CDF and containment performance, and (3) strengths and weaknesses of the models and methods used in the IPEs. These perspectives are gained by assessing the core damage and containment performance results, including overall CDF, accident sequences, dominant contributions to component failure and human error, and containment failure modes. Methods, data, boundary conditions, and assumptions used in the IPEs are considered in understanding the differences and similarities observed among the various types of plants. This report is divided into three volumes containing six parts. Part 1 is a summary report of the key perspectives gained in each of the areas identified above, with a discussion of the NRC`s overall conclusions and observations. Part 2 discusses key perspectives regarding the impact of the IPE Program on reactor safety. Part 3 discusses perspectives gained from the IPE results regarding CDF, containment performance, and human actions. Part 4 discusses perspectives regarding the IPE models and methods. Part 5 discusses additional IPE perspectives. Part 6 contains Appendices A, B and C which provide the references of the information from the IPEs, updated PRA results, and public comments on draft NUREG-1560 respectively.

  12. Final Project Report CFA-14-6357: A New Paradigm for Understanding Multiphase Ceramic Waste Form Performance

    Energy Technology Data Exchange (ETDEWEB)

    Brinkman, Kyle [Clemson Univ., SC (United States); Bordia, Rajendra [Clemson Univ., SC (United States); Reifsnider, Kenneth [Univ. of South Carolina, Columbia, SC (United States); Chiu, Wilson [Univ. of Connecticut, Storrs, CT (United States); Amoroso, Jake [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2017-12-28

    This project fabricated model multiphase ceramic waste forms with processing-controlled microstructures followed by advanced characterization with synchrotron and electron microscopy-based 3D tomography to provide elemental and chemical state-specific information resulting in compositional phase maps of ceramic composites. Details of 3D microstructural features were incorporated into computer-based simulations using durability data for individual constituent phases as inputs in order to predict the performance of multiphase waste forms with varying microstructure and phase connectivity.

  13. Simulation studies to evaluate the effect of fracture closure on the performance of fractured reservoirs; Final report

    Energy Technology Data Exchange (ETDEWEB)

    Howrie, I.; Dauben, D.

    1994-03-01

    A three-year research program to evaluate the effect of fracture closure on the recovery of oil and gas from naturally fractured reservoirs has been completed. The overall objectives of the study were to: (1) evaluate the reservoir conditions for which fracture closure is significant, and (2) evaluate innovative fluid injection techniques capable of maintaining pressure within the reservoir. The evaluations of reservoir performance were made by a modern dual porosity simulator, TETRAD. This simulator treats both porosity and permeability as functions of pore pressure. The Austin Chalk in the Pearsall Field in of South Texas was selected as the prototype fractured reservoir for this work. During the first year, simulations of vertical and horizontal well performance were made assuming that fracture permeability was insensitive to pressure change. Sensitivity runs indicated that the simulator was predicting the effects of critical reservoir parameters in a logical and consistent manner. The results confirmed that horizontal wells could increase both rate of oil recovery and total oil recovery from naturally fractured reservoirs. In the second year, the performance of the same vertical and horizontal wells was reevaluated with fracture permeability treated as a function of reservoir pressure. To investigate sensitivity to in situ stress, differing loading conditions were assumed. Simulated natural depletions confirm that pressure sensitive fractures degrade well performance. The severity of degradation worsens when the initial reservoir pressure approaches the average stress condition of the reservoir, such as occurs in over pressured reservoirs. Simulations with water injection indicate that degradation of permeability can be counteracted when reservoir pressure is maintained and oil recovery can be increased when reservoir properties are favorable.

  14. Individual plant examination program: Perspectives on reactor safety and plant performance. Parts 2--5: Final report; Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-01

    This report provides perspectives gained by reviewing 75 Individual Plant Examination (IPE) submittals pertaining to 108 nuclear power plant units. IPEs are probabilistic analyses that estimate the core damage frequency (CDF) and containment performance for accidents initiated by internal events. The US Nuclear Regulatory Commission (NRC) reviewed the IPE submittals with the objective of gaining perspectives in three major areas: (1) improvements made to individual plants as a result of their IPEs and the collective results of the IPE program, (2) plant-specific design and operational features and modeling assumptions that significantly affect the estimates of CDF and containment performance, and (3) strengths and weaknesses of the models and methods used in the IPEs. These perspectives are gained by assessing the core damage and containment performance results, including overall CDF, accident sequences, dominant contributions to component failure and human error, and containment failure modes. Methods, data, boundary conditions, and assumptions used in the IPEs are considered in understanding the differences and similarities observed among the various types of plants. This report is divided into three volumes containing six parts. Part 1 is a summary report of the key perspectives gained in each of the areas identified above, with a discussion of the NRC`s overall conclusions and observations. Part 2 discusses key perspectives regarding the impact of the IPE Program on reactor safety. Part 3 discusses perspectives gained from the IPE results regarding CDF, containment performance, and human actions. Part 4 discusses perspectives regarding the IPE models and methods. Part 5 discusses additional IPE perspectives. Part 6 contains Appendices A, B and C which provide the references of the information from the IPEs, updated PRA results, and public comments on draft NUREG-1560 respectively.

  15. Individual plant examination program: Perspectives on reactor safety and plant performance. Parts 2--5: Final report; Volume 2

    International Nuclear Information System (INIS)

    1997-12-01

    This report provides perspectives gained by reviewing 75 Individual Plant Examination (IPE) submittals pertaining to 108 nuclear power plant units. IPEs are probabilistic analyses that estimate the core damage frequency (CDF) and containment performance for accidents initiated by internal events. The US Nuclear Regulatory Commission (NRC) reviewed the IPE submittals with the objective of gaining perspectives in three major areas: (1) improvements made to individual plants as a result of their IPEs and the collective results of the IPE program, (2) plant-specific design and operational features and modeling assumptions that significantly affect the estimates of CDF and containment performance, and (3) strengths and weaknesses of the models and methods used in the IPEs. These perspectives are gained by assessing the core damage and containment performance results, including overall CDF, accident sequences, dominant contributions to component failure and human error, and containment failure modes. Methods, data, boundary conditions, and assumptions used in the IPEs are considered in understanding the differences and similarities observed among the various types of plants. This report is divided into three volumes containing six parts. Part 1 is a summary report of the key perspectives gained in each of the areas identified above, with a discussion of the NRC's overall conclusions and observations. Part 2 discusses key perspectives regarding the impact of the IPE Program on reactor safety. Part 3 discusses perspectives gained from the IPE results regarding CDF, containment performance, and human actions. Part 4 discusses perspectives regarding the IPE models and methods. Part 5 discusses additional IPE perspectives. Part 6 contains Appendices A, B and C which provide the references of the information from the IPEs, updated PRA results, and public comments on draft NUREG-1560 respectively

  16. Individual plant examination program: Perspectives on reactor safety and plant performance. Part 1: Final summary report; Volume 1

    International Nuclear Information System (INIS)

    1997-12-01

    This report provides perspectives gained by reviewing 75 Individual Plant Examination (IPE) submittals pertaining to 108 nuclear power plant units. IPEs are probabilistic analyses that estimate the core damage frequency (CDF) and containment performance for accidents initiated by internal events. The US Nuclear Regulatory Commission (NRC) reviewed the IPE submittals with the objective of gaining perspectives in three major areas: (1) improvements made to individual plants as a result of their IPEs and the collective results of the IPE program, (2) plant-specific design and operational features and modeling assumptions that significantly affect the estimates of CDF and containment performance, and (3) strengths and weaknesses of the models and methods used in the IPEs. These perspectives are gained by assessing the core damage and containment performance results, including overall CDF, accident sequences, dominant contributions to component failure and human error, and containment failure modes. Methods, data, boundary conditions, and assumptions used in the IPEs are considered in understanding the differences and similarities observed among the various types of plants. This report is divided into three volumes containing six parts. Part 1 is a summary report of the key perspectives gained in each of the areas identified above, with a discussion of the NRC's overall conclusions and observations. Part 2 discusses key perspectives regarding the impact of the IPE Program on reactor safety. Part 3 discusses perspectives gained from the IPE results regarding CDF, containment performance, and human actions. Part 4 discusses perspectives regarding the IPE models and methods. Part 5 discusses additional IPE perspectives. Part 6 contains Appendices A, B and C which provide the references of the information from the IPEs, updated PRA results, and public comments on draft NUREG-1560 respectively

  17. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    R Paul Drake

    2004-01-12

    OAK-B135 This is the final report from the project Hydrodynamics by High-Energy-Density Plasma Flow and Hydrodynamics and Radiation Hydrodynamics with Astrophysical Applications. This project supported a group at the University of Michigan in the invention, design, performance, and analysis of experiments using high-energy-density research facilities. The experiments explored compressible nonlinear hydrodynamics, in particular at decelerating interfaces, and the radiation hydrodynamics of strong shock waves. It has application to supernovae, astrophysical jets, shock-cloud interactions, and radiative shock waves.

  18. To reflect or not to reflect: Prior team performance as a boundary condition of the effects of reflexivity on learning and final team performance.

    NARCIS (Netherlands)

    Schippers, M.; Homan, A.C.; van Knippenberg, D.

    2013-01-01

    A small but growing body of literature adds to our understanding of the role of team reflexivity (i.e., reflecting upon team functioning) in predicting team performance. Although many studies conclude that reflexivity is an asset for teams, the contingencies of team reflexivity have received far

  19. Biodiesel Performance with Modern Engines. Cooperative Research and Development Final Report, CRADA Number CRD-05-153

    Energy Technology Data Exchange (ETDEWEB)

    McCormick, Robert [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-01-29

    NREL and the National Biodiesel Board (NBB) will work cooperatively to assess the effects of biodiesel blends on the performance of modern diesel engines and emissions control systems meeting increasingly strict emissions standards. This work will include research to understand the impact of biodiesel blends on the operation and durability of particle filters and NOx control sorbents/catalysts, to quantify the effect on emission control systems performance, and to understand effects on engine component durability. Work to assess the impact of biodiesel blends on real world fleet operations will be performed. Also, research to develop appropriate ASTM standards for biodiesel quality and stability will be conducted. The cooperative project will involve engine testing and fleet evaluation studies at NREL using biodiesel from a variety of sources. In addition, NREL will work with NBB to set up an Industrial Steering Committee to design the scope for the various projects and to provide technical oversight to these projects. NREL and NBB will cooperatively communicate the study results to as broad an audience as possible.

  20. Radioimmunoscintigraphy with monoclonal antibody Technetium-99m-Anti-EGF-Receptor (R3-MAB) for the detection of head and neck tumours, metastasis and recurrence. Final report for the period 15 April 1995 - 15 April 1997

    International Nuclear Information System (INIS)

    Oliva Gonzalez, J.P.

    1998-03-01

    A clinical study was carried out to determine the sensitivity of radioimmunoscintigraphy (RIS) using indigenously produced mouse monoclonal antibody (MAB) against epidermal growth factor receptor in the detection of primary, recurrent and metastatic malignant epithelial tumours of the head and neck region in 13 patients. The MAB was labelled with 99m Tc and imaging was carried out using gamma camera and SPECT. The results were correlated with histopathological findings. RIS gave a sensitivity of 76.9%. This study showed that the indigenously produced MAB can be used for the detection of malignant epithelial tumours in the head and neck region but the MAB will be further characterized to improve its sensitivity in the detection of the neoplasia. (author)

  1. Clinical performance of antibodies to prothrombin and thrombin in Chinese patients with antiphospholipid syndrome: potential interest in discriminating patients with thrombotic events and non-thrombotic events.

    Science.gov (United States)

    Zhang, Shulan; Wu, Ziyan; Li, Jing; Li, Ping; Chen, Si; Wen, Xiaoting; Li, Liubing; Zhang, Wen; Zhao, Jiuliang; Zhang, Fengchun; Li, Yongzhe

    2017-04-01

    A hallmark feature of antiphospholipid syndrome (APS) is the presence of a wide spectrum of antiphospholipid antibodies. In this study, we evaluated the clinical relevance of antibodies to prothrombin (PT) (aPT) and thrombin (aThr) in Chinese patients with APS. A total of 229 subjects were tested, including 86 patients with APS [35 patients with primary APS (PAPS), 51 patients with APS associated with other diseases (APSAOD)], 104 patients with non-APS diseases (disease controls), and 39 healthy controls. Serum IgG/IgM/IgA aPT and aThr were determined by ELISA. The levels of both IgG/IgM/IgA aPT and IgG/IgM/IgA aThr were significantly increased in patients with PAPS and APSAOD compared with patients with non-APS thrombosis and non-APS PRM, and HC. Both IgG aPT and IgG aThr exhibited promising diagnostic potentials for APS with sensitivities and specificities of 16.3 and 95.8% (IgG aPT), and 19.8 and 99.3% (IgG aThr), respectively. Importantly, both IgG aPT (OR 4.06; 95% CI 1.49-11.05) and IgG aThr (OR 4.49; 95% CI 1.62-12.45) were significantly correlated with arterial, but not venous, thrombotic events. Our findings highlighted that IgG aPT and IgG aThr could serve as promising biomarkers to identify patients at risk of arterial thrombosis in China.

  2. Oxygen enriched combustion system performance study. Phase 2: 100 percent oxygen enriched combustion in regenerative glass melters, Final report

    Energy Technology Data Exchange (ETDEWEB)

    Tuson, G.B.; Kobayashi, H.; Campbell, M.J.

    1994-08-01

    The field test project described in this report was conducted to evaluate the energy and environmental performance of 100% oxygen enriched combustion (100% OEC) in regenerative glass melters. Additional objectives were to determine other impacts of 100% OEC on melter operation and glass quality, and to verify on a commercial scale that an on-site Pressure Swing Adsorption oxygen plant can reliably supply oxygen for glass melting with low electrical power consumption. The tests constituted Phase 2 of a cooperative project between the United States Department of Energy, and Praxair, Inc. Phase 1 of the project involved market and technical feasibility assessments of oxygen enriched combustion for a range of high temperature industrial heating applications. An assessment of oxygen supply options for these applications was also performed during Phase 1, which included performance evaluation of a pilot scale 1 ton per day PSA oxygen plant. Two regenerative container glass melters were converted to 100% OEC operation and served as host sites for Phase 2. A 75 ton per day end-fired melter at Carr-Lowrey Glass Company in Baltimore, Maryland, was temporarily converted to 100% OEC in mid- 1990. A 350 tpd cross-fired melter at Gallo Glass Company in Modesto, California was rebuilt for permanent commercial operation with 100% OEC in mid-1991. Initially, both of these melters were supplied with oxygen from liquid storage. Subsequently, in late 1992, a Pressure Swing Adsorption oxygen plant was installed at Gallo to supply oxygen for 100% OEC glass melting. The particular PSA plant design used at Gallo achieves maximum efficiency by cycling the adsorbent beds between pressurized and evacuated states, and is therefore referred to as a Vacuum/Pressure Swing Adsorption (VPSA) plant.

  3. Final report to NASA JSC : thermal abuse performance of MOLI, Panasonic and Sanyo 18650 Li-ion cells.

    Energy Technology Data Exchange (ETDEWEB)

    Roth, Emanuel Peter

    2005-03-01

    Thermal property measurements of 18650 cells for the Space Shuttle Orbiter Advanced Hydraulic Power System (AHPS, formerly known as EAPU) have been performed. Cells were measured from three commercial manufacturers: E-One MOLI (12 cells), Panasonic (5 cells) and Sanyo (5 cells). Thermal property measurements of the MOLI 18650 cells included measurements of specific heat, self discharge (microcalorimetry), overcharge response and thermal runaway by accelerating rate calorimetry (ARC). The Panasonic and Sanyo cells were measured only for thermal runaway response in the ARC at increasing states of charge (3.8V, 4.0V, 4.2V, 4.3V).

  4. Alliance for Computational Science Collaboration, HBCU Partnership at Alabama A&M University Final Performance Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Deng, Z.T.

    2001-11-15

    The objective of this project was to conduct high-performance computing research and teaching at AAMU, and to train African-American and other minority students and scientists in the computational science field for eventual employment with DOE. During the project period, eight tasks were accomplished. Student Research Assistant, Work Study, Summer Interns, Scholarship were proved to be one of the best ways for us to attract top-quality minority students. Under the support of DOE, through research, summer interns, collaborations, scholarships programs, AAMU has successfully provided research and educational opportunities to minority students in the field related to computational science.

  5. LL13-MatModelRadDetect-PD2Jf Final Report: Materials Modeling for High-Performance Radiation Detectors

    Energy Technology Data Exchange (ETDEWEB)

    Lordi, Vincenzo [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2017-12-11

    The aims of this project are to enable rational materials design for select high-payoff challenges in radiation detection materials by using state-of-the-art predictive atomistic modeling techniques. Three specific high-impact challenges are addressed: (i) design and optimization of electrical contact stacks for TlBr detectors to stabilize temporal response at room-temperature; (ii) identification of chemical design principles of host glass materials for large-volume, low-cost, highperformance glass scintillators; and (iii) determination of the electrical impacts of dislocation networks in Cd1-xZnxTe (CZT) that limit its performance and usable single-crystal volume. The specific goals are to establish design and process strategies to achieve improved materials for high performance detectors. Each of the major tasks is discussed below in three sections, which include the goals for the task and a summary of the major results, followed by a listing of publications that contain the full details, including details of the methodologies used. The appendix lists 12 conference presentations given for this project, including 1 invited talk and 1 invited poster.

  6. Effects of radiation and high heat flux on the performance of first-wall components. Final report

    International Nuclear Information System (INIS)

    Wolfer, W.G.

    1985-10-01

    The performance of high-heat-flux components in present and future fusion devices is strongly affected by materials properties and their changes with radiation exposure and helium content. In addition, plasma disruptions and thermal fatigue are major life-limiting aspects. A multidisciplinary approach is therefore required in the performance analysis, and the following results have been accomplished. An equation of state for helium has been derived and applied to helium bubble formation by various growth processes. Models for various radiation effects have been developed and perfected to analyze radiation-induced swelling and embrittlement for high-heat flux materials. Computer codes have been developed to predict melting, evaporation, and melt-layer stability during plasma disruptions. A structural analysis code was perfected to evaluate the stress distribution and crack propagation in a high-heat-flux component or first wall. This code was applied to a duplex structure consisting of a beryllium coating on a copper substrate. It was also used to compare the lifetimes of a first wall in a tokamak reactor made of ferritic or austenitic steel

  7. PV Inverter Products Manufacturing and Design Improvements for Cost Reduction and Performance Enhancements: Final Subcontract Report, November 2003 (Revised)

    Energy Technology Data Exchange (ETDEWEB)

    West, R.

    2004-04-01

    The specific objectives of this subcontracted development work by Xantrex Technology Inc. were to: (1) Capture the newest digital signal processor (DSP) technology to create high-impact,''next generation'' power conversion equipment for the PV industry; (2) Create a common resource base for three PV product lines. This standardized approach to both hardware and software control platforms will provide significant market advantage over foreign competition; (3) Achieve cost reductions through increased volume of common components, reduced assembly labor, and the higher efficiency of producing more products with fewer design, manufacturing, and production test variations; (4) Increase PV inverter product reliability. Reduce inverter size, weight and conversion losses. The contract goals were to achieve an overall cost reduction of 10% to 20% for the three inverters and with no compromise in performance. The cost of the 10-kW inverter was reduced by 56%, and the cost of the 25-kW inverter was reduced by 53%. The 2.5-kW inverter has no basis for comparison, but should benefit equally from this design approach. Not only were the contract cost reduction goals exceeded by a wide margin, but the performance and reliability of the products were also enhanced. The conversion efficiency improvement, as reflected in the 50% conversion loss reduction, adds significant value in renewable energy applications. The size and weight reductions also add value by providing less cumbersome product solutions for system designers.

  8. Performance and economic evaluation of the seahorse natural gas hot water heater conversion at Fort Stewart. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Winiarski, D.W.

    1995-12-01

    The Federal government is the largest single energy consumer in the United States with consumption of nearly 1.5 quads/year of energy (10{sup 15} quad = 1015 Btu) and cost valued at nearly $10 billion annually. The US Department of Energy`s (DOE) Federal Energy Management Program (FEMP) supports efforts to reduce energy use and associated expenses in the Federal sector. One such effort, the New Technology Demonstration Program (NTDP) seeks to evaluate new energy -- saving US technologies and secure their more timely adoption by the US government. Pacific Northwest Laboratory (PNL) is one of four DOE laboratories that participate in the New Technologies Demonstration Program, providing technical expertise and equipment to evaluate new, energy-saving technologies being studied under that program. This report provides the results of a field evaluation that PNL conducted for DOE/FEMP with funding support from the US Department of Defense (DoD) Strategic Environmental Research and Development Program (SERDP) to examine the performance of 4 candidate energy-saving technology-a water heater conversion system to convert electrically powered water heaters to natural gas fuel. The unit was installed at a single residence at Fort Stewart, a US Army base in Georgia, and the performance was monitored under the NTDP. Participating in this effort under a Cooperative Research and Development Agreement (CRADA) were Gas Fired Products, developers of the technology; the Public Service Company of North Carolina; Atlanta Gas Light Company; the Army Corps of Engineers; Fort Stewart; and Pacific Northwest Laboratory.

  9. Final Research Performance Progress Report: Geothermal Resource Development with Zero Mass Withdrawal, Engineered Convection, and Wellbore Energy Conversion

    Energy Technology Data Exchange (ETDEWEB)

    Hughes, Richard [Louisiana State Univ., Baton Rouge, LA (United States); Tyagi, Mayank [Louisiana State Univ., Baton Rouge, LA (United States); Radonjic, Mileva [Louisiana State Univ., Baton Rouge, LA (United States); Dahi, Arash [Louisiana State Univ., Baton Rouge, LA (United States); Wang, Fahui [Louisiana State Univ., Baton Rouge, LA (United States); John, Chacko [Louisiana State Univ., Baton Rouge, LA (United States); Kaiser, Mark [Louisiana State Univ., Baton Rouge, LA (United States); Snyder, Brian [Louisiana State Univ., Baton Rouge, LA (United States); Sears, Stephen [Louisiana State Univ., Baton Rouge, LA (United States)

    2017-07-07

    This project is intended to demonstrate the technical and economic feasibility, and environmental and social attractiveness of a novel method of heat extraction from geothermal reservoirs. The emphasis is on assessing the potential for a heat extraction method that couples forced and free convection to maximize extraction efficiency. The heat extraction concept is enhanced by considering wellbore energy conversion, which may include only a boiler for a working fluid, or perhaps a complete boiler, turbine, and condenser cycle within the wellbore. The feasibility of this system depends on maintaining mechanical and hydraulic integrity of the wellbore, so the material properties of the casing-cement system are examined both experimentally and with well design calculations. The attractiveness depends on mitigation of seismic and subsidence risks, economic performance, environmental impact, and social impact – all of which are assessed as components of this study.

  10. Iron-Based Amorphous-Metals: High-Performance Corrosion-Resistant Materials (HPCRM) Development Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Farmer, J C; Choi, J; Saw, C; Haslem, J; Day, D; Hailey, P; Lian, T; Rebak, R; Perepezko, J; Payer, J; Branagan, D; Beardsley, B; D' Amato, A; Aprigliano, L

    2009-03-16

    An overview of the High-Performance Corrosion-Resistant Materials (HPCRM) Program, which was co-sponsored by the Defense Advanced Research Projects Agency (DARPA) Defense Sciences Office (DSO) and the United States Department of Energy (DOE) Office of Civilian and Radioactive Waste Management (OCRWM), is discussed. Programmatic investigations have included a broad range of topics: alloy design and composition; materials synthesis; thermal stability; corrosion resistance; environmental cracking; mechanical properties; damage tolerance; radiation effects; and important potential applications. Amorphous alloys identified as SAM2X5 (Fe{sub 49.7}Cr{sub 17.7}Mn{sub 1.9}Mo{sub 7.4}W{sub 1.6}B{sub 15.2}C{sub 3.8}Si{sub 2.4}) and SAM1651 (Fe{sub 48}Mo{sub 14}Cr{sub 15}Y{sub 2}C{sub 15}B{sub 6}) have been produced as melt-spun ribbons, drop-cast ingots and thermal-spray coatings. Chromium (Cr), molybdenum (Mo) and tungsten (W) additions provided corrosion resistance, while boron (B) enabled glass formation. Earlier electrochemical studies of melt-spun ribbons and ingots of these amorphous alloys demonstrated outstanding passive film stability. More recently thermal-spray coatings of these amorphous alloys have been made and subjected to long-term salt-fog and immersion tests. Good corrosion resistance has been observed during salt-fog testing. Corrosion rates were measured in situ with linear polarization, while simultaneously monitoring the open-circuit corrosion potentials. Reasonably good performance was observed. The sensitivity of these measurements to electrolyte composition and temperature was determined. The high boron content of this particular amorphous metal make this amorphous alloy an effective neutron absorber, and suitable for criticality control applications. In general, the corrosion resistance of these iron-based amorphous metals is maintained at operating temperatures up to the glass transition temperature. These materials are much harder than conventional

  11. Final Research Performance Report - Small Molecular Associative Carbon Dioxide (CO2) Thickeners for Improved Mobility Control

    Energy Technology Data Exchange (ETDEWEB)

    Enick, Robert M. [Univ. of Pittsburgh, PA (United States)

    2017-12-31

    polymers can serve as a CO2-soluble conformance control agent for CO2-EOR, especially in sandstone formations. This injection of a single phase solution of CO2-PFA for permeability reduction is (to the best of our knowledge) the first report of a CO2-soluble conformance control additive. We also demonstrated that the optimal strategy for using CO2-PFA solutions for conformance control is analogous to the application of water-based polymeric gels; the CO2-PFA solution should first be injected only in an isolated thief zone to induce dramatic reductions in permeability only in that thief zone, and then CO2 should be injected into all of the zones. Finally, it was noted that given the propensity of PFA to adsorb onto sandstone, the adsorption of PFA from CO2-PFA solutions onto cement surfaces promote the sealing of extremely fine cracks in casing cement.

  12. Analysis to Inform CA Grid Integration Rules for PV: Final Report on Inverter Settings for Transmission and Distribution System Performance

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Jeff [Electric Power Research Inst. (EPRI), Palo Alto, CA (United States); Rylander, Matthew [Electric Power Research Inst. (EPRI), Palo Alto, CA (United States); Boemer, Jens [Electric Power Research Inst. (EPRI), Palo Alto, CA (United States); Broderick, Robert Joseph [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Reno, Matthew J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Mather, Barry [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-09-01

    The fourth solicitation of the California Solar Initiative (CSI) Research, Development, Demonstration and Deployment (RD&D) Program established by the California Public Utilities Commission (CPUC) supported the Electric Power Research Institute (EPRI), National Renewable Energy Laboratory (NREL), and Sandia National Laboratories (SNL) with data provided from Pacific Gas and Electric (PG&E), Southern California Edison (SCE), and San Diego Gas and Electric (SDG&E) conducted research to determine optimal default settings for distributed energy resource advanced inverter controls. The inverter functions studied are aligned with those developed by the California Smart Inverter Working Group (SIWG) and those being considered by the IEEE 1547 Working Group. The advanced inverter controls examined to improve the distribution system response included power factor, volt-var, and volt-watt. The advanced inverter controls examined to improve the transmission system response included frequency and voltage ride-through as well as Dynamic Voltage Support. This CSI RD&D project accomplished the task of developing methods to derive distribution focused advanced inverter control settings, selecting a diverse set of feeders to evaluate the methods through detailed analysis, and evaluating the effectiveness of each method developed. Inverter settings focused on the transmission system performance were also evaluated and verified. Based on the findings of this work, the suggested advanced inverter settings and methods to determine settings can be used to improve the accommodation of distributed energy resources (PV specifically). The voltage impact from PV can be mitigated using power factor, volt-var, or volt-watt control, while the bulk system impact can be improved with frequency/voltage ride-through.

  13. Spent Fuel Performance Assessment and Research. Final Report of a Coordinated Research Project on Spent Fuel Performance Assessment and Research (SPAR-III) 2009–2014

    International Nuclear Information System (INIS)

    2015-10-01

    At the beginning of 2014, there were 437 nuclear power reactors in operation and 72 reactors under construction. To date, around 370 500 t (HM) (tonnes of heavy metal) of spent fuel have been discharged from reactors, and approximately 253 700 t (HM) are stored at various storage facilities. Although wet storage at reactor sites still dominates, the amount of spent fuel being transferred to dry storage technologies has increased significantly since 2005. For example, around 28% of the total fuel inventory in the United States of America is now in dry storage. Although the licensing for the construction of geological disposal facilities is under way in Finland, France and Sweden, the first facility is not expected to be available until 2025 and for most States with major nuclear programmes not for several decades afterwards. Spent fuel is currently accumulating at around 7000 t (HM) per year worldwide. The net result is that the duration of spent fuel storage has increased beyond what was originally foreseen. In order to demonstrate the safety of both spent fuel and the storage system, a good understanding of the processes that might cause deterioration is required. To address this, the IAEA continued the Coordinated Research Project (CRP) on Spent Fuel Performance Assessment and Research (SPAR-III) in 2009 to evaluate fuel and materials performance under wet and dry storage and to assess the impact of interim storage on associated spent fuel management activities (such as handling and transport). This has been achieved through: evaluating surveillance and monitoring programmes of spent fuel and storage facilities; collecting and exchanging relevant experience of spent fuel storage and the impact on associated spent fuel management activities; facilitating the transfer of knowledge by documenting the technical basis for spent fuel storage; creating synergy among research projects of the participating Member States; and developing the capability to assess the impact

  14. High-performance ceramic filters for energy engineering. Final report; Filter aus Hochleistungskeramik fuer die Energietechnik. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Westerheide, R. [Fraunhofer-Institut fuer Werkstoffmechanik (IWM), Freiburg im Breisgau (Germany); Adler, J. [Fraunhofer-Institut fuer Keramische Technologien und Sinterwerkstoffe (IKTS), Dresden (Germany); Buhl, H. [ESK-SIC GmbH, Frechen-Grefrath (Germany); Fister, D. [H.C. Starck GmbH, Laufenburg (Germany); Krein, J. [LLB Lurgi Lentjes Energietechnik GmbH, Frankfurt (Germany); Voelker, W. [Annawerk GmbH, Roedental (Germany); Walch, A. [eds.] [USF Schumacher Umwelt- und Trenntechnik GmbH, Crailsheim (Germany)

    1999-09-30

    The hot gas particulate removal of many advanced coal fired power generation technologies works at temperatures above 800 C. The filter elements for these applications are often based on ceramic materials, e.g. silicon carbide. However, the mostly clay bonded silicon carbide is subject to creep and oxidation due to probable changes of the binder phase. In this work the development of new ceramic filter materials based on silicon carbide and alumina is described. The goal of the development was to increase the potential application temperature. To obtain the goal, the work was performed together with ceramic powder manufacturers, developers of ceramic materials and components as well as with companies who operate test facilities. Different routes were chosen to increase the high temperature resistance in consideration of corrosion resistance, fracture strength and pressure loss of the filter materials. One of these routes was the optimization of the binder phase of the silicon carbide materials. Other routes were concentrated on the base material and the investigation of other possibilities for the silicon carbide bonding, i.e. a recrystallization process of SiC (RSiC) or a self bonding of granulated small grained silicon carbide powder. Additionally filter materials based on alumina were developed. The report covers these material development oriented topics as well as the additional work in materials reliability, coating development and modeling of microstructure. (orig.) [German] In der Kombikraftwerkstechnik wird insbesondere bei Kohlefeuerung die Heissgasreinigung oft bei Temperaturen ueber 800 C eingesetzt. Die Filterelemente fuer diese Anwendungen bestehen oft aus keramischen Materialien. Das haeufig eingesetzte tongebundene Siliciumcarbid unterliegt jedoch besonders aufgrund der Beschaffenheit der Bindephase Kriech- und Oxidationsschaedigungen. In diesem Bericht wird die Entwicklung von neuen keramischen Filtermaterialien, die auf Siliciumcarbid oder

  15. Two years' performance of an in-house ELISA for diagnosis of Legionnaires' disease: detection of specific IgM and IgG antibodies against Legionella pneumophila serogroup 1, 3 and 6 in human serum.

    Science.gov (United States)

    Elverdal, P L; Jørgensen, C S; Krogfelt, K A; Uldum, S A

    2013-08-01

    The aim of this study was to evaluate the performance of an in-house ELISA for the diagnosis of Legionnaires' disease (LD) by detection of IgM and IgG antibodies against Legionella (L.) pneumophila serogroups (sg) 1, 3 and 6. The evaluation was done throughout a two-year period in a diagnostic routine laboratory. Furthermore, the sensitivity of four different methods, the in-house L. pneumophila antibody test (ELISA), the urinary antigen test (Binax® EIA), an in-house PCR and culture, both alone and in combination was evaluated. From 2008 to 2010, 12,158 serum samples from 10,503 patients were analysed. During the same period, 361 cases of laboratory-confirmed LD cases were recorded in Denmark, but of these only 113 had a serum sample examined. The positive predictive value of the in-house ELISA was calculated to be 12.8 and the negative predictive value was 99.6, using only the confirmed LD cases as true positives. The sensitivity of the in-house ELISA for the detection of IgM and IgG antibodies in the confirmed LD cases was 61% and 36%, respectively. By combining the two ELISA assays the sensitivity increased to 66%. The sensitivity of the Legionella urinary antigen test (Binax® EIA) was 63%, of the in-house PCR 87% and of culture 69%. When all the different methods were combined, a higher sensitivity was calculated--for in-house ELISA (IgM+IgG) and Binax® EIA 91%, in-house ELISA (IgM+IgG) and in-house PCR 93%, in-house ELISA (IgM+IgG) and culture 93%, Binax® EIA and in-house PCR 79%, Binax® EIA and culture 68% and in-house PCR and culture 94%. This study confirms that the detection of IgG and IgM antibodies by ELISA is an important diagnostic tool, also during the initial phase of the disease. Furthermore, we showed that LD in Denmark with or without serum samples collected exhibits the same age and sex distribution and epidemiology, as in the rest of Europe, i.e., mostly men are infected, infections are mostly community acquired, followed by infection from

  16. High temperature process steam application at the Southern Union Refining Company, Hobbs, New Mexico. Solar energy in the oil patch. Final report, Phase III: operation, maintenance, and performance

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, L.E.; McGuire, D.R.

    1984-05-01

    This final report summarizes the technical reports for Phase III of this project. The third phase included the operation, maintenance, upgrade and performance reporting of a 10,080 square foot Solar Industrial Process Heat System installed at the Famariss Energy Refinery of Southern Union Refining Company near Hobbs, New Mexico. This report contains a description of the upgraded system, and a summary of the overall operation, maintenance and performance of the installed system. The results of the upgrade activities can be seen in the last two months of operational data. Steam production was significantly greater in peak flow and monthly total than at any previous time. Also monthly total cost savings was greatly improved even though natural gas costs remain much lower than originally anticipated.

  17. IMPORTANCE OF RESEARCH HLA ANTIBODIES CLASS I AND II, AND MICA ANTIBODIES IN KIDNEY TRANSPLANTATION

    Directory of Open Access Journals (Sweden)

    M. Sh. Khubutia

    2011-01-01

    Full Text Available The purpose of this study was to investigate the occurrence of HLA and MICA antibodies in patients from the waiting list for kidney transplantation and their influence on the course of post-transplant period. Determination of HLA antibodies class I and II, and MICA antibodies was performed on a platform of Luminex (xMAP-tech- nology using sets LABScreen ONE LAMBDA (U.S.. A total of 156 patients from the waiting list for kidney transplantation. Revealed the presence of HLA and MICA antibodies in the serum of 31.4% of patients. Regraf- ted patients increased the content of antibodies to the antigens of HLA system was noted in 88.2% of cases, 47% met the combination of antibodies to the I, II classes and MICA. In patients awaiting first kidney transplantation, HLA and MICA antibodies were determined in 23.7% of cases. The presence of pretransplant HLA and MICA antibodies had a significant influence on the course of post-transplant period. Patients with the presence of HLA and MICA in 50% of cases delayed graft function. Sessions of plasmapheresis can reduce the concentration of HLA and MICA antibodies on average by 61.1%. 

  18. Antibodies Against Melanin

    African Journals Online (AJOL)

    1973-01-06

    Jan 6, 1973 ... Departments of Internal Medicine and Anatomical Pathology, University of Stellenbosch and MRC. Pigment Metabolism Research Unit, ... at the production of antibodies against natural melanoprotein. and a consideration of our negative .... the random polymerization of several monomers, antibody formed ...

  19. Recombinant renewable polyclonal antibodies.

    Science.gov (United States)

    Ferrara, Fortunato; D'Angelo, Sara; Gaiotto, Tiziano; Naranjo, Leslie; Tian, Hongzhao; Gräslund, Susanne; Dobrovetsky, Elena; Hraber, Peter; Lund-Johansen, Fridtjof; Saragozza, Silvia; Sblattero, Daniele; Kiss, Csaba; Bradbury, Andrew R M

    2015-01-01

    Only a small fraction of the antibodies in a traditional polyclonal antibody mixture recognize the target of interest, frequently resulting in undesirable polyreactivity. Here, we show that high-quality recombinant polyclonals, in which hundreds of different antibodies are all directed toward a target of interest, can be easily generated in vitro by combining phage and yeast display. We show that, unlike traditional polyclonals, which are limited resources, recombinant polyclonal antibodies can be amplified over one hundred million-fold without losing representation or functionality. Our protocol was tested on 9 different targets to demonstrate how the strategy allows the selective amplification of antibodies directed toward desirable target specific epitopes, such as those found in one protein but not a closely related one, and the elimination of antibodies recognizing common epitopes, without significant loss of diversity. These recombinant renewable polyclonal antibodies are usable in different assays, and can be generated in high throughput. This approach could potentially be used to develop highly specific recombinant renewable antibodies against all human gene products.

  20. Immunoglobulin Classification Using the Colored Antibody Graph.

    Science.gov (United States)

    Bonissone, Stefano R; Pevzner, Pavel A

    2016-06-01

    The somatic recombination of V, D, and J gene segments in B-cells introduces a great deal of diversity, and divergence from reference segments. Many recent studies of antibodies focus on the population of antibody transcripts that show which V, D, and J gene segments have been favored for a particular antigen, a repertoire. To properly describe the antibody repertoire, each antibody must be labeled by its constituting V, D, and J gene segment, a task made difficult by somatic recombination and hypermutation events. While previous approaches to repertoire analysis were based on sequential alignments, we describe a new de Bruijn graph-based algorithm to perform VDJ labeling and benchmark its performance.

  1. Preliminary parametric performance assessment of potential final waste forms for alpha low-level waste at the Idaho National Engineering Laboratory. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Smith, T.H.; Sussman, M.E. [Lockheed Idaho Technologies Co., Idaho Falls, ID (United States); Myers, J.; Djordjevic, S.M.; DeBiase, T.A.; Goodrich, M.T.; DeWitt, D. [IT Corp., Albuquerque, NM (United States)

    1995-08-01

    This report presents a preliminary parametric performance assessment (PA) of potential waste disposal systems for alpha-contaminated, mixed, low-level waste (ALLW) currently stored at the Transuranic Storage Area of INEL. The ALLW, which contains from 10 to 100 nCi/g of transuranic (TRU) radionuclides, is awaiting treatment and disposal. The purpose of this study was to examine the effects of several parameters on the radiological-confinement performance of potential disposal systems for the ALLW. The principal emphasis was on the performance of final waste forms (FWFs). Three categories of FWF (cement, glass, and ceramic) were addressed by evaluating the performance of two limiting FWFs for each category. Performance at five conceptual disposal sites was evaluated to illustrate the effects of site characteristics on the performance of the total disposal system. Other parameters investigated for effects on receptor dose included inventory assumptions, TRU radionuclide concentration, FWF fracture, disposal depth, water infiltration rates, subsurface-transport modeling assumptions, receptor well location, intrusion scenario assumptions, and the absence of waste immobilization. These and other factors were varied singly and in some combinations. The results indicate that compliance of the treated and disposed ALLW with the performance objectives depends on the assumptions made, as well as on the FWF and the disposal site. Some combinations result in compliance, while others do not. The implications of these results for decision making relative to treatment and disposal of the INEL ALLW are discussed. The report compares the degree of conservatism in this preliminary parametric PA against that in four other PAs and one risk assessment. All of the assessments addressed the same disposal site, but different wastes. The report also presents a qualitative evaluation of the uncertainties in the PA and makes recommendations for further study.

  2. Preliminary parametric performance assessment of potential final waste forms for alpha low-level waste at the Idaho National Engineering Laboratory. Revision 1

    International Nuclear Information System (INIS)

    Smith, T.H.; Sussman, M.E.; Myers, J.; Djordjevic, S.M.; DeBiase, T.A.; Goodrich, M.T.; DeWitt, D.

    1995-08-01

    This report presents a preliminary parametric performance assessment (PA) of potential waste disposal systems for alpha-contaminated, mixed, low-level waste (ALLW) currently stored at the Transuranic Storage Area of INEL. The ALLW, which contains from 10 to 100 nCi/g of transuranic (TRU) radionuclides, is awaiting treatment and disposal. The purpose of this study was to examine the effects of several parameters on the radiological-confinement performance of potential disposal systems for the ALLW. The principal emphasis was on the performance of final waste forms (FWFs). Three categories of FWF (cement, glass, and ceramic) were addressed by evaluating the performance of two limiting FWFs for each category. Performance at five conceptual disposal sites was evaluated to illustrate the effects of site characteristics on the performance of the total disposal system. Other parameters investigated for effects on receptor dose included inventory assumptions, TRU radionuclide concentration, FWF fracture, disposal depth, water infiltration rates, subsurface-transport modeling assumptions, receptor well location, intrusion scenario assumptions, and the absence of waste immobilization. These and other factors were varied singly and in some combinations. The results indicate that compliance of the treated and disposed ALLW with the performance objectives depends on the assumptions made, as well as on the FWF and the disposal site. Some combinations result in compliance, while others do not. The implications of these results for decision making relative to treatment and disposal of the INEL ALLW are discussed. The report compares the degree of conservatism in this preliminary parametric PA against that in four other PAs and one risk assessment. All of the assessments addressed the same disposal site, but different wastes. The report also presents a qualitative evaluation of the uncertainties in the PA and makes recommendations for further study

  3. Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, India

    Directory of Open Access Journals (Sweden)

    Rucha Patki

    2017-01-01

    Full Text Available Objective. The aim of this study was to establish a baseline titre for the population of Mumbai, Maharashtra, India. Method. Four hundred healthy blood donors, attending blood donation camps, were screened using a survey questionnaire. Widal tube agglutination test was performed on the diluted sera (with 0.9% normal saline of blood donors, with final dilution ranging from 1 : 40 to 1 : 320. Results. Out of 400 individuals providing samples, 78 (19.5% individuals showed antibody titres ≥ 1 : 40 for at least one antigen and 322 (80.5% showed no agglutination. The baseline antibody titres against O antigen and H antigen of Salmonella enterica serotype Typhi were found to be 1 : 40 and 1 : 80, respectively. Similarly, the baseline antibody titres for the H antigen of Salmonella enterica serotypes Paratyphi A and Paratyphi B were found to be 1 : 40 and 1 : 80, respectively. Conclusion. Thus, it was noted that the diagnostically significant cutoff of antibody titre from acute phase sample was ≥ 1 : 80 for S. Typhi O antigen and titre of ≥ 1 : 160 for both S. Typhi H antigen and S. Paratyphi BH antigen. Antibody titre of ≥ 1 : 80 can be considered significant for S. Paratyphi AH antigen.

  4. Baseline Antibody Titre againstSalmonella entericain Healthy Population of Mumbai, Maharashtra, India.

    Science.gov (United States)

    Patki, Rucha; Lilani, Sunil; Lanjewar, Dhaneshwar

    2017-01-01

    The aim of this study was to establish a baseline titre for the population of Mumbai, Maharashtra, India. Four hundred healthy blood donors, attending blood donation camps, were screened using a survey questionnaire. Widal tube agglutination test was performed on the diluted sera (with 0.9% normal saline) of blood donors, with final dilution ranging from 1 : 40 to 1 : 320. Out of 400 individuals providing samples, 78 (19.5%) individuals showed antibody titres ≥ 1 : 40 for at least one antigen and 322 (80.5%) showed no agglutination. The baseline antibody titres against O antigen and H antigen of Salmonella enterica serotype Typhi were found to be 1 : 40 and 1 : 80, respectively. Similarly, the baseline antibody titres for the H antigen of Salmonella enterica serotypes Paratyphi A and Paratyphi B were found to be 1 : 40 and 1 : 80, respectively. Thus, it was noted that the diagnostically significant cutoff of antibody titre from acute phase sample was ≥ 1 : 80 for S. Typhi O antigen and titre of ≥ 1 : 160 for both S. Typhi H antigen and S. Paratyphi BH antigen. Antibody titre of ≥ 1 : 80 can be considered significant for S. Paratyphi AH antigen.

  5. Design of an Expression System for Rapid Production of Tri-Functional Antibody Substitution of Hybrid Hybridoma Technology

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Dehghani

    2018-03-01

    Full Text Available Tri-functional antibodies, as an effective novel tumor targeting agents, are composed of anti-CD3 rat IgG2b and an anti-tumor antigen antibody. We have intended to develop a novel drug system to induce the apoptosis of HER2-expressing tumor cells, activate and engage cytotoxic T lymphocytes, natural killer cells, and macrophages. In addition, the drug can  inhibit programmed death ligand 1 (PDL-1-expressing tumor cells. We have designed a mammalian vector system suitable to express the trifunctional antibody composed of antiHER2×human-CD80: human-IgG1Fc antibody. This antibody contains four chains of anti-HER2/CL, anti-HER2/CH1-3, B7.1/CL, and B7.1/CH1-3 within the human IgG1 framework and so the vector system will simultaneously express the four chains. The amino acid/nucleotide sequences datasets were retrieved from the GenBank, UniProtKB and PDB databases. The heavy and light chains variable domain framework regions and complementarity determining regions of scA21 antibody were determined by IMGT/V-QUEST and Paratome software. The amino acid sequences of tri-functional antibody chains manually were assembled and converted to DNA sequences by sequence manipulation suite software. The adapting codon usage of these DNA sequences was performed by JCAT software. Finally, the secondary structures of obtained RNAs from the DNAs were individually analyzed by RNAfold program. The Prodigy equilibrium dissociation constant, a ratio of koff/kon, between the antibody and its antigen for hantiHER2VHCH-HER2, hantiHER2VLCL-HER2, CD80CH-CD28, and CD80CL-CD28 were equal to 3.60E-10, 3.10E-9, 1.10E-8 and 2.70E-10; respectively. These findings were confirmed the composition and nano-molar affinity of the respective constructs. A single specific no-cloning expression vector, pHuchiTriomAb, was designed in silico with a desirable length of 8.292 Kbps and bidirectional expression potential for the four chimeric antibody chains. This construct was designed, and

  6. Total System Performance Assessment - Analyses for Disposal of Commercial and DOE Waste Inventories at Yucca Mountain - Input to Final Environmental Impact Statement and Site Suitability Evaluation, Rev. 00

    International Nuclear Information System (INIS)

    NA

    2001-01-01

    This Letter Report presents the results of calculations to assess long-term performance of commercial spent nuclear fuel (CSNF), U.S. Department of Energy (DOE) spent nuclear fuel (DSNF), high-level radioactive waste (HLW), and Greater Than Class C (GTCC) radioactive waste and DOE Special Performance Assessment Required (SPAR) radioactive waste at the potential Yucca Mountain repository in Nye County Nevada with respect to the 10,000-year performance period specified in 40 CFR Part 197.30 (66 FR 32074 [DIRS 155216], p. 32134) with regard to radiation-protection standards. The EPA Final Rule 40 CFR Part 197 has three separate standards, individual-protection, human-intrusion, and groundwater-protection standards, all with a compliance timeframe of 10,000 years. These calculations evaluate the dose to receptors for each of these standards. Further, this Letter Report includes the results of simulations to the 1,000,000-year performance period described in 40 CFR Part 197.35 (66 FR 32074 [DIRS 155216], p. 32135) which calls for the calculation of the peak dose to the Reasonably Maximally Exposed Individual (RMEI) that would occur after 10,000 years and within the period of geological stability. In accordance with TSPA-SR the ''period of geologic stability'' is from zero to 1,000,000 years after repository closure. The calculations also present the 5th and 95th percentiles, and the mean and median of the set of probabilistic simulations used to evaluate various disposal scenarios

  7. Bispecific T-Cell Engager (BiTE) Antibody Construct Blinatumomab for the Treatment of Patients With Relapsed/Refractory Non-Hodgkin Lymphoma: Final Results From a Phase I Study.

    Science.gov (United States)

    Goebeler, Maria-Elisabeth; Knop, Stefan; Viardot, Andreas; Kufer, Peter; Topp, Max S; Einsele, Hermann; Noppeney, Richard; Hess, Georg; Kallert, Stefan; Mackensen, Andreas; Rupertus, Kathrin; Kanz, Lothar; Libicher, Martin; Nagorsen, Dirk; Zugmaier, Gerhard; Klinger, Matthias; Wolf, Andreas; Dorsch, Brigitte; Quednau, Beate D; Schmidt, Margit; Scheele, Jürgen; Baeuerle, Patrick A; Leo, Eugen; Bargou, Ralf C

    2016-04-01

    Blinatumomab is a CD19/CD3 BiTE (bispecific T-cell engager) antibody construct for the treatment of Philadelphia chromosome-negative acute B-lymphoblastic leukemia. We evaluated blinatumomab in relapsed/refractory B-cell non-Hodgkin lymphoma (NHL). This 3 + 3 design, phase I dose-escalation study determined adverse events and the maximum tolerated dose (MTD) of continuous intravenous infusion blinatumomab in patients with relapsed/refractory NHL. Blinatumomab was administered over 4 or 8 weeks at seven different dose levels (0.5 to 90 μg/m(2)/day). End points were incidence of adverse events, pharmacokinetics, pharmacodynamics, and overall response rate. Between 2004 and 2011, 76 heavily pretreated patients with relapsed/refractory NHL, who included 14 with diffuse large B-cell lymphoma, were enrolled; 42 received treatment in the formal dose-escalation phase. Neurologic events were dose limiting, and 60 μg/m(2)/day was established as the MTD. Thirty-four additional patients were recruited to evaluate antilymphoma activity and strategies for mitigating neurologic events at a prespecified MTD. Stepwise dosing (5 to 60 μg/m(2)/day) plus pentosan polysulfate SP54 (n = 3) resulted in no treatment discontinuations; single-step (n = 5) and double-step (n = 24) dosing entailed two and seven treatment discontinuations due to neurologic events, respectively. Grade 3 neurologic events occurred in 22% of patients (no grade 4/5). Among patients treated at 60 μg/m(2)/day (target dose; n = 35), the overall response rate was 69% across NHL subtypes and 55% for diffuse large B-cell lymphoma (n = 11); median response duration was 404 days (95% CI, 207 to 1,129 days). In this phase I study of relapsed/refractory NHL, continuous infusion with CD19-targeted immunotherapy blinatumomab at various doses and schedules was feasible, with an MTD of 60 μg/m(2)/day. Single-agent blinatumomab showed antilymphoma activity. © 2016 by American Society of Clinical Oncology.

  8. Antibody engineering: methods and protocols

    National Research Council Canada - National Science Library

    Chames, Patrick

    2012-01-01

    "Antibody Engineering: Methods and Protocols, Second Edition was compiled to give complete and easy access to a variety of antibody engineering techniques, starting from the creation of antibody repertoires and efficient...

  9. Anti-insulin antibody test

    Science.gov (United States)

    Insulin antibodies - serum; Insulin Ab test; Insulin resistance - insulin antibodies; Diabetes - insulin antibodies ... You appear to have an allergic response to insulin Insulin no longer seems to control your diabetes

  10. Monoclonal antibody "gold rush".

    Science.gov (United States)

    Maggon, Krishan

    2007-01-01

    The market, sales and regulatory approval of new human medicines, during the past few years, indicates increasing number and share of new biologics and emergence of new multibillion dollar molecules. The global sale of monoclonal antibodies in 2006 were $20.6 billion. Remicade had annual sales gain of $1 billion during the past 3 years and five brands had similar increase in 2006. Rituxan with 2006 sales of $4.7 billion was the best selling monoclonal antibody and biological product and the 6th among the top selling medicinal brand. It may be the first biologic and monoclonal antibody to reach $10 billion annual sales in the near future. The strong demand from cancer and arthritis patients has surpassed almost all commercial market research reports and sales forecast. Seven monoclonal antibody brands in 2006 had sales exceeding $1 billion. Humanized or fully human monoclonal antibodies with low immunogenicity, enhanced antigen binding and reduced cellular toxicity provide better clinical efficacy. The higher technical and clinical success rate, overcoming of technical hurdles in large scale manufacturing, low cost of market entry and IND filing, use of fully human and humanized monoclonal antibodies has attracted funds and resources towards R&D. Review of industry research pipeline and sales data during the past 3 years indicate a real paradigm shift in industrial R&D from pharmaceutical to biologics and monoclonal antibodies. The antibody bandwagon has been joined by 200 companies with hundreds of new projects and targets and has attracted billions of dollars in R&D investment, acquisitions and licensing deals leading to the current Monoclonal Antibody Gold Rush.

  11. Experience in the Development of the CMS Inner Tracker Analog Optohybrid Circuits: Project, Qualification, Volume Production, Quality Assurance and Final Performance

    CERN Document Server

    Ricci, Daniel; Bilei, Gian Mario; Casinini, F; Postolache, Vasile

    2005-01-01

    The Tracker system of the Compact Muon Solenoid (CMS) Experiment, will employ approximately 40,000 analog fibre-optic data and control links. The optical readout system is responsible for converting and transmitting the electrical signals coming out from the front-end to the outside counting room. Concerning the inner part of the Tracker, about 3,600 Analog Optohybrid circuits are involved in this tasks. These circuits have been designed and successfully produced in Italy under the responsibility of INFN Perugia CMS group completing the volume production phase by February 2005. Environmental features, reliability and performances of these circuits have been extensively tested and qualified. This paper reviews the most relevant steps of the manufacturing and quality assurance process: from prototypes to mass-production for the final CMS use.

  12. Anti-nuclear antibodies positive serum from systemic lupus erythematosus patients promotes cardiovascular manifestations and the presence of human antibody in the brain.

    Science.gov (United States)

    Kelly-Worden, Marie; Hammer, Leslie; Gebhard, Robyn; Schrader, Lauran; Griffin, Marley; Cooper, Dalahnna

    2014-07-01

    Systemic lupus erythematosus (SLE) is characterized by the presence of anti-nuclear antibodies (ANAs) in the serum of patients. These antibodies may cross over into the brain resulting in the development of neuropsychiatric symptoms and result in abnormal pathology in other organs such as the heart and kidneys. The objective of this study was to determine if SLE pathology could be detected in the hearts and brains of rats injected with positive human ANA serum. Lewis rats (n = 31) were selected for this study due to documented research already performed with this strain in the investigation of serum sickness, encephalitis and autoimmune related carditis. Rats were injected once a week with either ANA positive or negative control serum or saline. Hearts were examined for initial signs of heart disease including the presence of lipid deposits, vegetation, increased ventricular thickness and a change in heart weight. Brains were examined for the presence of human antibody and necrotic lesions. Animals were observed for outward signs of neuropathy as well. Blood samples were taken in order to determine final circulating concentrations of IgG and monitor histamine levels. Animals injected with ANA were significantly higher for lipid deposits in the heart and an increased ventricular thickness was noted. One animal even displayed Libman-Sacks endocarditis. Brains were positive for the presence of human IgG and diffuse internal lesions occurred in 80% of the ANA positive serum injected animals examined. Blood histamine levels were not significantly different, but actually lower than controls by the end of the experiment. Since human antibodies were detected in the brain, further studies will have to identify which antibody cross reactions are occurring within the brain, examine cell infiltration as well as characterize the antibodies associated with more destructive consequences such as lesion formation.

  13. Antibody affinity maturation

    DEFF Research Database (Denmark)

    Skjødt, Mette Louise

    surface expression of various antibody formats in the generated knockout strain. Functional scFv and scFab fragments were efficiently displayed on yeast whereas impaired chain assembly and heavy chain degradation was observed for display of full-length IgG molecules. To identify the optimal polypeptide......-antibody interface and the antibody intraface.the microenvironment and ecology of Acaryochloris and Prochloron, and in this thesis we attempted to further describe the distribution, growth characteristics and adaptive/regulatory mechanisms of these two cyanobacteria, both in their natural habitat and under defined...

  14. Targeting Malignant Brain Tumors with Antibodies

    Directory of Open Access Journals (Sweden)

    Rok Razpotnik

    2017-09-01

    -based therapy using the antibody to bind to the specific target(s. Finally, the current clinical trials are reviewed, showing the most recent progress of attractive approaches to deliver therapeutic antibodies across the BBB aiming at the specific antigen.

  15. Effect of adding crushed Pimpinella anisum, Nigella sativa seeds and Thymus vulgaris mixture to antibiotics-free rations of vaccinated and non-vaccinated male broilers on growth performance, antibody titer and haematological profile

    Directory of Open Access Journals (Sweden)

    Mamoun Z. Athamneh

    2010-04-01

    Full Text Available This research explores an experimental study conducted to investigate the effect of crushed Pimpinella anisum (PA, Nigella sativa (NS seeds and Thymus vulgaris (TV mixture as a feed additive on growth performance and mortality rate (MR, selected antibodies titer (Ab’s and blood hematological profile of vaccinated and non-vaccinated Lohman male broiler chicks fed free-antibiotics ration. A total of 400 one-day old chicks were distributed into 16 groups (4 treatment x 4 replicates x 25chicks. The experiment lasted from one to 42 days of age. The statistical findings of this experiment prove that the use of medicinal plants mixture improves live body weight, body weight gain, feed conversion ratio and MR of vaccinated male broilers at 21 and 42 days of age. antibodies titer against infectious bronchitis and infectious bursal disease of non-vaccinated and vaccinated male broilers were significantly improved at 21 and 42 days as a result of the addition of medicinal plant mixture to the basal ration. Concerning Newcastle disease, the use of PA, NS and TV mixture did not reflect in any additional improvement of Ab's than vaccines did. The addition of medicinal plants mixture increases WBC's, RBC's, thrombocytes count and Hb concentration of vaccinated and non-vaccinated male broilers at 21 days of age. Meanwhile, heterophils, lymphocytes and monocytes of vaccinated male broilers (VMB were significantly improved by adding medicinal plant mixture to their basal diet. Moreover, at 42 days of age the use of PA, NS seeds and TV mixture indicate significant increase in total WBC’s, lymphocytes and monocytes and monocytes count of VMB and non-vaccinated male broiler (NVMB. No significant differences were noticed in RBC’s and Hct as a result of feeding crushed medicinal plants mixture.

  16. Anti-sulfotyrosine antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Bertozzi, Carolyn R [Berkeley, CA; Kehoe, John [Saint Davids, PA; Bradbury, Andrew M [Santa Fe, NM

    2009-09-15

    The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

  17. Antibody Blood Tests

    Science.gov (United States)

    Antibody Blood Tests Researchers have discovered that people with celiac disease who eat gluten have higher than normal levels of ... do I do if I have a negative blood test (or panel) but I’m still having symptoms? ...

  18. Total System Performance Assessment - Analyses for Disposal of Commercial and DOE Waste Inventories at Yucca Mountain - Input to Final Environmental Impact Statement and Site Suitability Evaluation, Rev. 00

    Energy Technology Data Exchange (ETDEWEB)

    NA

    2001-09-17

    This Letter Report presents the results of calculations to assess long-term performance of commercial spent nuclear fuel (CSNF), U.S. Department of Energy (DOE) spent nuclear fuel (DSNF), high-level radioactive waste (HLW), and Greater Than Class C (GTCC) radioactive waste and DOE Special Performance Assessment Required (SPAR) radioactive waste at the potential Yucca Mountain repository in Nye County Nevada with respect to the 10,000-year performance period specified in 40 CFR Part 197.30 (66 FR 32074 [DIRS 155216], p. 32134) with regard to radiation-protection standards. The EPA Final Rule 40 CFR Part 197 has three separate standards, individual-protection, human-intrusion, and groundwater-protection standards, all with a compliance timeframe of 10,000 years. These calculations evaluate the dose to receptors for each of these standards. Further, this Letter Report includes the results of simulations to the 1,000,000-year performance period described in 40 CFR Part 197.35 (66 FR 32074 [DIRS 155216], p. 32135) which calls for the calculation of the peak dose to the Reasonably Maximally Exposed Individual (RMEI) that would occur after 10,000 years and within the period of geological stability. In accordance with TSPA-SR the ''period of geologic stability'' is from zero to 1,000,000 years after repository closure. The calculations also present the 5th and 95th percentiles, and the mean and median of the set of probabilistic simulations used to evaluate various disposal scenarios.

  19. Bartonella species antibodies and hyperglobulinemia in privately owned cats.

    Science.gov (United States)

    Whittemore, J C; Hawley, J R; Radecki, S V; Steinberg, J D; Lappin, M R

    2012-01-01

    Bartonella species are zoonotic agents and primary pathogens in cats. Hyperglobulinemia has been associated with bartonellosis in humans and cats. To evaluate for associations between Bartonella species immunoglobulin G (IgG) antibodies and serum biochemistry panel results in privately owned cats. 1,477 privately owned cats. Residual sera were collected after biochemical evaluation for this prospective, cross-sectional serosurvey. Bartonella species IgG ELISA was performed with a cutoff value of ≥ 1 : 64. Stepwise logistic regression analysis was performed with the endpoint titer as the outcome variable. The final statistical model included age, albumin, ALP activity, ALT activity, bilirubin, creatinine, glucose, and globulin as covariates. Serum protein electrophoresis was performed with serum from 50 cats with and without antibodies to Bartonella species and hyperglobulinemia. Sera from cats seropositive to Bartonella species and with hyperglobulinemia were assessed for evidence of exposure to other infectious agents associated with hyperglobulinemia. Risk of seropositivity to Bartonella species was positively associated with the natural log of globulin concentration (OR = 11.90, 95% CI 6.15-23.02, P glucose concentration (OR = 0.66, 95% CI 0.50-0.87, P = .004). Another explanation for hyperglobulinemia was not identified for most cats with Bartonella species antibodies. Hyperglobulinemia was primarily caused by polyclonal gammopathy in cats that were seronegative and seropositive for Bartonella species. Hyperglobulinemia was significantly associated with seropositivity to Bartonella species. Testing for bartonellosis is warranted in cats with unexplained hyperglobulinemia and clinical or laboratory findings suggestive of bartonellosis. Copyright © 2012 by the American College of Veterinary Internal Medicine.

  20. Supplementation of a Blend of Beneficial Bacteria and Antibodies on Growth Performance, Intestinal Mucosa Morphology and Right Heart Failure of Japanese Quail (Coturnix coturnix japonica

    Directory of Open Access Journals (Sweden)

    Mohammad Hasan Mehraei Hamzekolaei

    2017-05-01

    Full Text Available Background: Early nutrition of chicks with beneficial bacteria might help in occupying the inner surface of the intestinal tract. Interference of pathogens in intestinal microbiota is well known as barrier effect, bacterial interference, and competitive exclusion. Objectives: It was hypothesized that competitive exclusion in Japanese quails with a blend of beneficial bacteria (Aquablend Avian® probiotic would enhance quails’ growth performance and intestinal mucosal morphology. Furthermore, the study was performed at 2100 m above sea level at Shahrekord University, so another hypothesis was the capability of the probiotic for inhibiting right heart failure. Materials and Methods: One hundred fifty-six Japanese quails were divided into 4 groups: 2 groups (Aquablend and control at standard environmental temperature and 2 (Aqua-stress and Cont-stress at cold-hypoxic environmental situation. Aquablend groups received the probiotic in the first 3 days of life in drinking water (0.5 g/100 birds/day. Results: Feed conversion ratio (FCR was significantly reduced at the end of the experiment (day 35 in both Aquablend and aqua-stress groups compared to control and cont-stress groups, respectively (P 0.05. Cont-stress group had higher RV: TV ratio (0.28 and heterophil: lymphocyte (H: L ratio (1.22 than aqua-stress group: (0.25 and (1.20, respectively (P > 0.05. Data regarding to intestinal mucosa morphology was controversial but the probiotic was able to elevate duodenum villi surface (P < 0.05 and also jejunum and ileum lamina propria thickness. Conclusion: Obtained data suggests that addition of Aquablend Avian® probiotic in the first 3 days of life may improve growth performance and some intestinal mucosa characteristics of Japanese quails. Moreover, the probiotic might reduce right heart failure and stress induced by cold-hypoxic situation.

  1. Effects of an amyloid-beta 1-42 oligomers antibody screened from a phage display library in APP/PS1 transgenic mice.

    Science.gov (United States)

    Wang, Jianping; Li, Nan; Ma, Jun; Gu, Zhiqiang; Yu, Lie; Fu, Xiaojie; Liu, Xi; Wang, Jian

    2016-03-15

    We screened anti-Aβ1-42 antibodies from a human Alzheimer's disease (AD) specific single chain variable fragment (scFv) phage display library and assessed their effects in APP/PS1 transgenic mice. Reverse transcription-PCR was used to construct the scFv phage display library, and screening identified 11A5 as an anti-Aβ1-42 antibody. We mixed 11A5 and the monoclonal antibody 6E10 with Aβ1-42 and administered the mixture to Sprague-Dawley rats via intracerebroventricular injection. After 30 days, rats injected with the antibody/Aβ1-42 mixture and those injected with Aβ1-42 alone were tested on the Morris water maze. We also injected 11A5 and 6E10 into APP/PS1 transgenic mice and assessed the concentrations of Aβ in brain and peripheral blood by ELISA at 1-month intervals for 3 months. Finally we evaluated behavior changes in the Morris water maze. Rats injected with Aβ1-42 and mixed antibodies showed better performance in the Morris water maze than did rats injected with Aβ1-42 alone. In APP/PS1 transgenic mice, Aβ concentration was lower in the brains of the antibody-treated group than in the control group, but higher in the peripheral blood. The antibody-treated mice also exhibited improved behavioral performance in the Morris water maze. In conclusion, anti-Aβ1-42 antibodies (11A5) screened from the human scFv antibody phage display library promoted the efflux or clearance of Aβ1-42 and effectively decreased the cerebral Aβ burden in an AD mouse model. Copyright © 2016 Elsevier B.V. All rights reserved.

  2. Improved diagnostic performance of a commercial anaplasma antibody competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5–glutathione S-transferase fusion protein as antigen

    Science.gov (United States)

    This study tested the hypothesis that removal of maltose binding protein from recombinant antigen used for plate coating would improve the specificity of Anaplasma antibody competitive ELISA. Three hundred and eight sera with significant MBP antibody binding (=30%I) in Anaplasma negative herds was 1...

  3. Warm antibody autoimmune hemolytic anemia.

    Science.gov (United States)

    Kalfa, Theodosia A

    2016-12-02

    Autoimmune hemolytic anemia (AIHA) is a rare and heterogeneous disease that affects 1 to 3/100 000 patients per year. AIHA caused by warm autoantibodies (w-AIHA), ie, antibodies that react with their antigens on the red blood cell optimally at 37°C, is the most common type, comprising ∼70% to 80% of all adult cases and ∼50% of pediatric cases. About half of the w-AIHA cases are called primary because no specific etiology can be found, whereas the rest are secondary to other recognizable underlying disorders. This review will focus on the postulated immunopathogenetic mechanisms in idiopathic and secondary w-AIHA and report on the rare cases of direct antiglobulin test-negative AIHA, which are even more likely to be fatal because of inherent characteristics of the causative antibodies, as well as because of delays in diagnosis and initiation of appropriate treatment. Then, the characteristics of w-AIHA associated with genetically defined immune dysregulation disorders and special considerations on its management will be discussed. Finally, the standard treatment options and newer therapeutic approaches for this chronic autoimmune blood disorder will be reviewed. © 2016 by The American Society of Hematology. All rights reserved.

  4. Natural and Man-made Antibody Repertories for Antibody Discovery

    Directory of Open Access Journals (Sweden)

    Juan C eAlmagro

    2012-11-01

    Full Text Available Antibodies are the fastest-growing segment of the biologics market. The success of antibody-based drugs resides in their exquisite specificity, high potency, stability, solubility, safety and relatively inexpensive manufacturing process in comparison with other biologics. We outline here the structural studies and fundamental principles that define how antibodies interact with diverse targets. We also describe the antibody repertoires and affinity maturation mechanisms of human, mice and chickens, plus the use of novel single-domain antibodies in camelids and sharks. These species all utilize diverse evolutionary solutions to generate specific and high affinity antibodies and illustrate the plasticity of natural antibody repertoires. In addition, we discuss the multiple variations of man-made antibody repertoires designed and validated in the last two decades, which have served as tools to explore how the size, diversity and composition of a repertoire impact the antibody discovery process.

  5. Schedulability Analysis for Java Finalizers

    DEFF Research Database (Denmark)

    Bøgholm, Thomas; Hansen, Rene Rydhof; Ravn, Anders P.

    2010-01-01

    Java finalizers perform clean-up and finalisation of objects at garbage collection time. In real-time Java profiles the use of finalizers is either discouraged (RTSJ, Ravenscar Java) or even disallowed (JSR-302), mainly because of the unpredictability of finalizers and in particular their impact ...... programs. Finally, we extend the SARTS tool for automated schedulability analysis of Java bytecode programs to handle finalizers in a fully automated way.......Java finalizers perform clean-up and finalisation of objects at garbage collection time. In real-time Java profiles the use of finalizers is either discouraged (RTSJ, Ravenscar Java) or even disallowed (JSR-302), mainly because of the unpredictability of finalizers and in particular their impact...... on the schedulability analysis. In this paper we show that a controlled scoped memory model results in a structured and predictable execution of finalizers, more reminiscent of C++ destructors than Java finalizers. Furthermore, we incorporate finalizers into a (conservative) schedulability analysis for Predictable Java...

  6. Antibody informatics for drug discovery

    DEFF Research Database (Denmark)

    Shirai, Hiroki; Prades, Catherine; Vita, Randi

    2014-01-01

    to the antibody science in every project in antibody drug discovery. Recent experimental technologies allow for the rapid generation of large-scale data on antibody sequences, affinity, potency, structures, and biological functions; this should accelerate drug discovery research. Therefore, a robust bioinformatic...... infrastructure for these large data sets has become necessary. In this article, we first identify and discuss the typical obstacles faced during the antibody drug discovery process. We then summarize the current status of three sub-fields of antibody informatics as follows: (i) recent progress in technologies...... for antibody rational design using computational approaches to affinity and stability improvement, as well as ab-initio and homology-based antibody modeling; (ii) resources for antibody sequences, structures, and immune epitopes and open drug discovery resources for development of antibody drugs; and (iii...

  7. Monoclonal antibodies targeting CD38 in hematological malignancies and beyond

    DEFF Research Database (Denmark)

    van de Donk, Niels W C J; Janmaat, Maarten L.; Mutis, Tuna

    2016-01-01

    CD38 is a multifunctional cell surface protein that has receptor as well as enzyme functions. The protein is generally expressed at low levels on various hematological and solid tissues, while plasma cells express particularly high levels of CD38. The protein is also expressed in a subset of hema...... strong anti-tumor activity in preclinical models. The antibody engages diverse mechanisms of action, including complement-dependent cytotoxicity, antibody-dependent cellular cytotoxicity, antibody-dependent cellular phagocytosis, programmed cell death, modulation of enzymatic activity...... combination therapies with existing as well as emerging therapies, which are currently evaluated in the clinic. Finally, CD38 antibodies may have a role in the treatment of diseases beyond hematological malignancies, including solid tumors and antibody-mediated autoimmune diseases. © 2016 John Wiley & Sons A....../S. Published by John Wiley & Sons Ltd....

  8. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    DeTar, Carleton [P.I.

    2012-12-10

    This document constitutes the Final Report for award DE-FC02-06ER41446 as required by the Office of Science. It summarizes accomplishments and provides copies of scientific publications with significant contribution from this award.

  9. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Gurney, Kevin R. [Arizona Univ., Mesa, AZ (United States)

    2015-01-12

    This document constitutes the final report under DOE grant DE-FG-08ER64649. The organization of this document is as follows: first, I will review the original scope of the proposed research. Second, I will present the current draft of a paper nearing submission to Nature Climate Change on the initial results of this funded effort. Finally, I will present the last phase of the research under this grant which has supported a Ph.D. student. To that end, I will present the graduate student’s proposed research, a portion of which is completed and reflected in the paper nearing submission. This final work phase will be completed in the next 12 months. This final workphase will likely result in 1-2 additional publications and we consider the results (as exemplified by the current paper) high quality. The continuing results will acknowledge the funding provided by DOE grant DE-FG-08ER64649.

  10. Antithyroglobulin Antibodies and Antimicrosomal Antibodies in Various Thyroid Diseases

    International Nuclear Information System (INIS)

    Lee, Gwon Jun; Hong, Key Sak; Choi, Kang Won; Lee, Kyu; Koh, Chang Soon; Lee, Mun Ho; Park, Sung Hoe; Chi, Je Geun; Lee, Sang Kook

    1979-01-01

    The authors investigated the incidence of antithyroglobulin antibodies and antibodies and antimicrosomal antibodies measured by tanned red cell hemagglutination method in subjects suffering from various thyroid disorders. 1) In 15 normal patients, neither suffering from any thyroid diseases nor from any other autoimmune disorders, the antithyroglobulin antibodies were all negative, but the antimicrosomal antibody was positive only in one patient (6.7%). 2) The antithyroglobulin antibodies were positive in 31.5% (34 patients) of 108 patients with various thyroid diseases, and the antimicrosomal antibodies were positive in 37.0% (40 patients). 3) of the 25 patients with Graves' diseases, 7 patients (28.0%) showed positive for the antithyroglobulin antibodies, and 9 (36.0%) for the antimicrosomal antibodies. There was no definite differences in clinical and thyroid functions between the groups with positive and negative results. 4) Both antibodies were positive in 16 (88.9%) and 17 (94.4%) patients respectively among 18 patients with Hashimoto's thyroiditis, all of them were diagnosed histologically. 5) Three out of 33 patients with thyroid adenoma showed positive antibodies, and 3 of 16 patients with thyroid carcinoma revealed positive antibodies. 6) TRCH antibodies demonstrated negative results in 2 patients with subacute thyroiditis, but positive in one patient with idiopathic primary myxedema. 7) The number of patients with high titers(>l:802) was 16 for antithyroglobulin antibody, and 62.5% (10 patients) of which was Hashimoto's thyroiditis. Thirteen (65.0) of 20 patients with high titers (>l:802) for antimicrosomal antibody was Hashimoto's thyroiditis. TRCH test is a simple, sensitive method, and has high reliability and reproducibility. The incidences and titers of antithyroglobulin antibody and antimicrosomal antibody are especially high in Hashimoto's thyroiditis.

  11. Paraneoplastic cerebellar syndromes associated with antibodies against Purkinje cells.

    Science.gov (United States)

    Schwenkenbecher, Philipp; Chacko, Lisa; Pul, Refik; Sühs, Kurt-Wolfram; Wegner, Florian; Wurster, Ulrich; Stangel, Martin; Skripuletz, Thomas

    2017-12-18

    The paraneoplastic cerebellar syndrome presents as severe neuroimmunological disease associated with malignancies. Antibodies against antigens expressed by tumor cells cross-react with proteins of cerebellar Purkinje cells leading to neuroinflammation and neuronal loss. These antineuronal antibodies are preferentially investigated by serological analyses while examination of the cerebrospinal fluid is only performed infrequently. We retrospectively investigated 12 patients with antineuronal antibodies against Purkinje cells with a special focus on cerebrospinal fluid. Our results confirm a subacute disease with a severe cerebellar syndrome in 10 female patients due to anti-Yo antibodies associated mostly with gynecological malignancies. While standard cerebrospinal fluid parameters infrequently revealed pathological results, all patients presented oligoclonal bands indicating intrathecal IgG synthesis. Analyses of anti-Yo antibodies in cerebrospinal fluid by calculating the antibody specific index revealed intrathecal synthesis of anti-Yo antibodies in these patients. In analogy to anti-Yo syndrome, an intrathecal production of anti-Tr antibodies in one patient who presented with a paraneoplastic cerebellar syndrome was detected. In an additional patient, anti-Purkinje cell antibodies of unknown origin in the cerebrospinal fluid but not in serum were determined suggesting an isolated immune reaction within the central nervous system (CNS) and underlining the importance of investigating the cerebrospinal fluid. In conclusion, patients with a cerebellar syndrome display a distinct immune reaction within the cerebrospinal fluid including intrathecal synthesis of disease-specific antibodies. We emphasize the importance of a thorough immunological work up including investigations of both serum and cerebrospinal fluid.

  12. Clinical relevance of HLA donor-specific antibodies detected by single antigen assay in kidney transplantation.

    Science.gov (United States)

    Caro-Oleas, José Luis; González-Escribano, María Francisca; González-Roncero, Francisco Manuel; Acevedo-Calado, María José; Cabello-Chaves, Virginia; Gentil-Govantes, Miguel Ángel; Núñez-Roldán, Antonio

    2012-03-01

    Clinical relevance of donor-specific antibodies (DSAs) detected by a single antigen Luminex virtual crossmatch in pre-transplant serum samples from patients with a negative cytotoxicity-dependent complement crossmatch is controversial. The aim of this study was to analyse the influence of a pre-transplant positive virtual crossmatch in the outcome of kidney transplantation. A total of 892 patients who received a graft from deceased donors after a negative cytotoxicity crossmatch were included. Presence of anti-human leucocyte antigen (HLA) antibodies was investigated using a Luminex screening assay and anti-HLA specificities were assigned performing a Luminex single antigen assay. Graft survival was significantly worse among patients with anti-HLA DSA compared to both patients with anti-HLA with no DSA (P = 0.001) and patients without HLA antibodies (P HLA with no DSA and no HLA antibodies patient groups were observed (P = 0.595). Influence of both anti-Class I and anti-Class II DSA was detected (P 1500 (global P > 0.05). The presence of preformed HLA DSA in transplanted patients with a negative cytotoxicity crossmatch is associated with a lower allograft survival. The detection of anti-HLA with no DSA has no influence in the graft outcome. Finally, there were no demonstrable effects of mean fluorescence intensity (MFI) values >1500 on graft survival.

  13. Detection and identification of platelet antibodies using a sensitive multiplex assay system-platelet antibody bead array.

    Science.gov (United States)

    Metzner, Krista; Bauer, Julie; Ponzi, Heather; Ujcich, Allison; Curtis, Brian R

    2017-07-01

    Tests for platelet-specific antibodies are important in the diagnosis of immune platelet disorders. Monoclonal antibody glycoprotein capture assays have been the gold standards in platelet antibody detection for almost 30 years. However, such assays are complex and cumbersome to perform, which limits their routine use. We report the performance of a newly developed, easy to perform platelet antibody bead array (PABA) for the detection of platelet-specific antibodies. PABA is the equivalent of the monoclonal antigen capture enzyme-linked immunosorbent assay (ELISA) (MACE) on a bead and instead with fluorescent detection of immunoglobulin (Ig)G platelet antibodies by Luminex. Antibodies against platelet glycoproteins (GP) GPIIb/IIIa, GPIb/IX, GPIa/IIa, GPIV, and class I human leukocyte antigen (HLA) could be detected in a patient's serum simultaneously in a single well of a microplate. Results from 80 patient samples and 20 normal donor samples were compared using PABA, MACE, and a commercial ELISA kit. PABA detected all antibodies, with specificity for human platelet antigens (HPAs) HPA-1a, HPA-1b, HPA-2a, HPA-2b, HPA-3a, HPA-3b, HPA-4a, HPA-4b, HPA-5a, HPA-5b, HPA-15b, and HLA. Overall, compared with MACE, the sensitivity and specificity of PABA were 99% and 97%, respectively, and both were significantly better than those of the commercial ELISA. PABA had greater analytic sensitivity than MACE for HPA-1a, HPA-3a, and HPA-5b antibodies. In addition, PABA detected HPA-5b and HPA-3b antibodies that were missed by MACE. The overall false-positive rate of PABA compared with MACE was 2.7%. The PABA is a rapid, highly sensitive and specific, multiplex bead-based assay for detecting human platelet antibodies. Such a simple yet high-throughput platform will facilitate practical, routine testing for the identification of platelet-specific antibodies. © 2017 AABB.

  14. Compositions, antibodies, asthma diagnosis methods, and methods for preparing antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Hongjun; Zangar, Richard C.

    2017-01-17

    Methods for preparing an antibody are provided with the method including incorporating 3-bromo-4-hydroxy-benzoic acid into a protein to form an antigen, immunizing a mammalian host with the antigen, and recovering an antibody having an affinity for the antigen from the host. Antibodies having a binding affinity for a monohalotyrosine are provided as well as composition comprising an antibody bound with monohalotyrosine. Compositions comprising a protein having a 3-bromo-4-hydroxy-benzoic acid moiety are also provided. Methods for evaluating the severity of asthma are provide with the methods including analyzing sputum of a patient using an antibody having a binding affinity for monohalotyrosine, and measuring the amount of antibody bound to protein. Methods for determining eosinophil activity in bodily fluid are also provided with the methods including exposing bodily fluid to an antibody having a binding affinity for monohalotyrosine, and measuring the amount of bound antibody to determine the eosinophil activity.

  15. Development of Polyclonal Antibody against Clenbuterol for Immunoassay Application

    Directory of Open Access Journals (Sweden)

    Nurul Ain A. Talib

    2018-03-01

    Full Text Available Development of an immunoassay for clenbuterol (CLB detection required an anti-CLB antibody as an important bioreceptor. In this study, we report our work on production and purification of a rabbit-derived polyclonal anti-CLB antibody. The antibody was then purified by nProtein A Sepharose affinity column and the antibody purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE analysis. The activities of purified antibody were evaluated based on high antibody titer determined from enzyme-linked immunosorbent assay (ELISA. The sensitivity and selectivity of this antibody was evaluated and exhibits negligible cross-reactivity to antibiotics other than β-agonist families. Evaluation of the antibody as bioreceptor in immunoassay was performed using direct competitive ELISA and exhibited linear calibration plot (R2 = 0.9484. The antibody was used to detect the content of CLB in spiked milk samples and the recovery of more than 92% indicating significant performance as bioreceptor for the development of a rapid and simple immunoassay.

  16. Impact of Uniform Methods on Interlaboratory Antibody Titration Variability: Antibody Titration and Uniform Methods.

    Science.gov (United States)

    Bachegowda, Lohith S; Cheng, Yan H; Long, Thomas; Shaz, Beth H

    2017-01-01

    -Substantial variability between different antibody titration methods prompted development and introduction of uniform methods in 2008. -To determine whether uniform methods consistently decrease interlaboratory variation in proficiency testing. -Proficiency testing data for antibody titration between 2009 and 2013 were obtained from the College of American Pathologists. Each laboratory was supplied plasma and red cells to determine anti-A and anti-D antibody titers by their standard method: gel or tube by uniform or other methods at different testing phases (immediate spin and/or room temperature [anti-A], and/or anti-human globulin [AHG: anti-A and anti-D]) with different additives. Interlaboratory variations were compared by analyzing the distribution of titer results by method and phase. -A median of 574 and 1100 responses were reported for anti-A and anti-D antibody titers, respectively, during a 5-year period. The 3 most frequent (median) methods performed for anti-A antibody were uniform tube room temperature (147.5; range, 119-159), uniform tube AHG (143.5; range, 134-150), and other tube AHG (97; range, 82-116); for anti-D antibody, the methods were other tube (451; range, 431-465), uniform tube (404; range, 382-462), and uniform gel (137; range, 121-153). Of the larger reported methods, uniform gel AHG phase for anti-A and anti-D antibodies had the most participants with the same result (mode). For anti-A antibody, 0 of 8 (uniform versus other tube room temperature) and 1 of 8 (uniform versus other tube AHG), and for anti-D antibody, 0 of 8 (uniform versus other tube) and 0 of 8 (uniform versus other gel) proficiency tests showed significant titer variability reduction. -Uniform methods harmonize laboratory techniques but rarely reduce interlaboratory titer variance in comparison with other methods.

  17. Antithyroid microsomal antibody

    Science.gov (United States)

    ... that you have a higher chance of developing thyroid disease in the future. Antithyroid microsomal antibodies may be ... PA: Elsevier; 2016:chap 11. Weiss RE, Refetoff S. Thyroid function testing. In: Jameson JL, De Groot LJ, eds. Endocrinology: Adult and ... Lupus Read more ...

  18. Antibodies Targeting EMT

    Science.gov (United States)

    2017-10-01

    determine their targets on the cell. The newly discovered antibodies will then be engineered for utility as new highly specific drugs and diagnostics in...are from the aldo-keto reductase family (AKRs). Remarkably, 3 of the top 10 genes with induction in the mesenchymal TES2b cells Figure 1. Amino

  19. Monoclonal antibodies in haematopathology

    Energy Technology Data Exchange (ETDEWEB)

    Grignani, F.; Martelli, M.F.; Mason, D.Y.

    1985-01-01

    This book contains over 40 selections. Some of the titles are: Oncogene (c-myc, c-myb) amplification in acute myelogenous leukaemia; Ultrastructural characterization of leukaemic cells with monoloclonal antibodies; Origin of B-cell malignancies; Immunohistology of gut lymphomas; and Spurious evidence of lineage infidelity in monocytic leukaemia.

  20. Monoclonal antibodies in myeloma

    DEFF Research Database (Denmark)

    Sondergeld, P.; van de Donk, N. W. C. J.; Richardson, P. G.

    2015-01-01

    The development of monoclonal antibodies (mAbs) for the treatment of disease goes back to the vision of Paul Ehrlich in the late 19th century; however, the first successful treatment with a mAb was not until 1982, in a lymphoma patient. In multiple myeloma, mAbs are a very recent and exciting add...

  1. Narrative Finality

    Directory of Open Access Journals (Sweden)

    Armine Kotin Mortimer

    1981-01-01

    Full Text Available The cloturai device of narration as salvation represents the lack of finality in three novels. In De Beauvoir's Tous les hommes sont mortels an immortal character turns his story to account, but the novel makes a mockery of the historical sense by which men define themselves. In the closing pages of Butor's La Modification , the hero plans to write a book to save himself. Through the thrice-considered portrayal of the Paris-Rome relationship, the ending shows the reader how to bring about closure, but this collective critique written by readers will always be a future book. Simon's La Bataille de Pharsale , the most radical attempt to destroy finality, is an infinite text. No new text can be written. This extreme of perversion guarantees bliss (jouissance . If the ending of De Beauvoir's novel transfers the burden of non-final world onto a new victim, Butor's non-finality lies in the deferral to a future writing, while Simon's writer is stuck in a writing loop, in which writing has become its own end and hence can have no end. The deconstructive and tragic form of contemporary novels proclaims the loss of belief in a finality inherent in the written text, to the profit of writing itself.

  2. Humanized Antibodies for Antiviral Therapy

    Science.gov (United States)

    Co, Man Sung; Deschamps, Marguerite; Whitley, Richard J.; Queen, Cary

    1991-04-01

    Antibody therapy holds great promise for the treatment of cancer, autoimmune disorders, and viral infections. Murine monoclonal antibodies are relatively easy to produce but are severely restricted for therapeutic use by their immunogenicity in humans. Production of human monoclonal antibodies has been problematic. Humanized antibodies can be generated by introducing the six hypervariable regions from the heavy and light chains of a murine antibody into a human framework sequence and combining it with human constant regions. We humanized, with the aid of computer modeling, two murine monoclonal antibodies against herpes simplex virus gB and gD glycoproteins. The binding, virus neutralization, and cell protection results all indicate that both humanized antibodies have retained the binding activities and the biological properties of the murine monoclonal antibodies.

  3. Defining process design space for monoclonal antibody cell culture.

    Science.gov (United States)

    Abu-Absi, Susan Fugett; Yang, LiYing; Thompson, Patrick; Jiang, Canping; Kandula, Sunitha; Schilling, Bernhard; Shukla, Abhinav A

    2010-08-15

    The concept of design space has been taking root as a foundation of in-process control strategies for biopharmaceutical manufacturing processes. During mapping of the process design space, the multidimensional combination of operational variables is studied to quantify the impact on process performance in terms of productivity and product quality. An efficient methodology to map the design space for a monoclonal antibody cell culture process is described. A failure modes and effects analysis (FMEA) was used as the basis for the process characterization exercise. This was followed by an integrated study of the inoculum stage of the process which includes progressive shake flask and seed bioreactor steps. The operating conditions for the seed bioreactor were studied in an integrated fashion with the production bioreactor using a two stage design of experiments (DOE) methodology to enable optimization of operating conditions. A two level Resolution IV design was followed by a central composite design (CCD). These experiments enabled identification of the edge of failure and classification of the operational parameters as non-key, key or critical. In addition, the models generated from the data provide further insight into balancing productivity of the cell culture process with product quality considerations. Finally, process and product-related impurity clearance was evaluated by studies linking the upstream process with downstream purification. Production bioreactor parameters that directly influence antibody charge variants and glycosylation in CHO systems were identified.

  4. Antilipide antibodies cofactor in syphilis, Lyme borreliosis and autoimmune diseases

    Directory of Open Access Journals (Sweden)

    B Voicechovskaya

    2003-01-01

    Full Text Available Objective. To study (32 glycoprotein 1 contribution to frequency of aCL in pts with autoimmune and some infectious diseases. Methods. Antibodies to different phospholipids were tested by immune-enzyme method (IEM in serum of 92 pts: 20 of them had systemic lupus erythematosus (SLE, 15 Sjogren’s disease (SD, 20 rheumatoid arthritis (RA, 20 syphilis and 17 Lyme disease (LD. Pts with syphilis and LD did not have clinical signs of autoimmune disease. Stimulation of aCL test in IEM was performed after previous cardiolipin incubation on microtitrated plates with electrophoretic pure p2 glycoprotein 1. Results. aCL were mostly revealed in serum of patients with SLE and syphilis, less often in SD. In aCL- positive serums antibodies to some other phospholipids were found. Mean values of stimulating aCL test in IEM after previous incubation with pure |32-glycoprotein 1 in autoimmune diseases were higher than in infection. aCL division into cofactor «dependent» and «independent» or autoimmune and infectious types seems to be not final.

  5. Structural Characterization of Peptide Antibodies

    DEFF Research Database (Denmark)

    Chailyan, Anna; Marcatili, Paolo

    2015-01-01

    The role of proteins as very effective immunogens for the generation of antibodies is indisputable. Nevertheless, cases in which protein usage for antibody production is not feasible or convenient compelled the creation of a powerful alternative consisting of synthetic peptides. Synthetic peptides...... can be modified to obtain desired properties or conformation, tagged for purification, isotopically labeled for protein quantitation or conjugated to immunogens for antibody production. The antibodies that bind to these peptides represent an invaluable tool for biological research and discovery...

  6. Synthetic peptides for antibody production

    NARCIS (Netherlands)

    N.D. Zegers (Netty)

    1995-01-01

    textabstractSynthetic peptides are useful tools for the generation of antibodies. The use of antibodies as specific reagents in inununochemical assays is widely applied. In this chapter, the application of synthetic peptides for the generation of antibodies is described. The different steps

  7. Synthetic peptides for antibody production

    NARCIS (Netherlands)

    Zegers, N.D.

    1995-01-01

    Synthetic peptides are useful tools for the generation of antibodies. The use of antibodies as specific reagents in inununochemical assays is widely applied. In this chapter, the application of synthetic peptides for the generation of antibodies is described. The different steps that lead to the

  8. Coxiella burnetii Circulation in a Naturally Infected Flock of Sheep: Individual Follow-Up of Antibodies in Serum and Milk.

    Science.gov (United States)

    Joulié, A; Rousset, E; Gasqui, P; Lepetitcolin, E; Leblond, A; Sidi-Boumedine, K; Jourdain, E

    2017-07-01

    The control of Q fever, a zoonotic disease caused by the Coxiella burnetii bacterium, remains a scientific challenge. Domestic ruminants are considered the main reservoir, shedding C. burnetii essentially through parturition products during abortion or birth. Sheep are particularly frequently associated with human outbreaks, but there are insufficient field data to fully understand disease dynamics and to instigate efficient control measures. A longitudinal follow-up study of a naturally infected sheep flock was performed (i) to investigate relationships between seropositivity and bacterial shedding in the vaginal mucus, (ii) to describe the kinetics of antibodies, including responses to vaccination, (iii) to monitor maternal antibodies in ewe lambs, and (iv) to compare serological results for milk and serum samples. For 8 months, we collected blood samples every 3 weeks from 11 aborting and 26 nonaborting dairy ewes, 20 nonaborting suckler ewes, and 9 ewe lambs. Individual milk samples were also obtained from lactating females. All serum and milk samples were tested by enzyme-linked immunosorbent assay (ELISA), whereas vaginal swabs were tested by quantitative PCR. We found that some dairy females did not seroconvert despite shedding C. burnetii in their vaginal mucus. Overall, antibody levels in adult females were found to remain stable over time, with exceptions during the mating and lambing periods. Maternal antibodies decreased during the first month after birth. Interestingly, antibody levels in milk were correlated with those in serum. This study provides valuable field data that will help improve Q fever surveillance and within-flock management measures. IMPORTANCE Field data are necessary to improve the surveillance, diagnosis, and sanitary management of Q fever in livestock. Here, we provide extensive serological data obtained from serum and milk samples from infected and vaccinated ewes belonging to a naturally infected flock of sheep. We show that

  9. Antibody-Mediated Catalysis in Infection and Immunity.

    Science.gov (United States)

    Bowen, Anthony; Wear, Maggie; Casadevall, Arturo

    2017-09-01

    The existence of catalytic antibodies has been known for decades. Natural antibodies capable of cleaving nucleic acid, protein, and polysaccharide substrates have been described. Although the discovery of catalytic antibodies initially aroused great interest because of their promise for the development of new catalysts, their enzymatic performance has been disappointing due to low reaction rates. However, in the areas of infection and immunity, where processes often occur over much longer times and involve high antibody concentrations, even low catalytic rates have the potential to influence biological outcomes. In this regard, the presence of catalytic antibodies recognizing host antigens has been associated with several autoimmune diseases. Furthermore, naturally occurring catalytic antibodies to microbial determinants have been correlated with resistance to infection. Recently, there has been substantial interest in harnessing the power of antibody-mediated catalysis against microbial antigens for host defense. Additional work is needed, however, to better understand the prevalence, function, and structural basis of catalytic activity in antibodies. Here we review the available information and suggest that antibody-mediated catalysis is a fertile area for study with broad applications in infection and immunity. Copyright © 2017 American Society for Microbiology.

  10. Clinical use of antibodies

    International Nuclear Information System (INIS)

    Baum, R.P.; Hoer, Gustav; Cox, P.H.; Buraggi, G.L.

    1991-01-01

    Use of monoclonal antibodies as tumour specific carrier molecules for therapeutic agents or as in vivo diagnostic reagents when labelled with radionuclides or NMR signal enhancers is attracting more and more attention. The potential is enormous but the technical problems are also considerable requiring the concerted action of many different scientific disciplines. This volume is based upon a symposium organised in Frankfurt in 1990 under the auspices of the European Association of Nuclear Medicines' Specialist Task Groups on Cardiology and the Utility of Labelled Antibodies. It gives a multidisciplinary review of the state of the art and of problems to be solved as well as recording the not inconsiderable successes which have been booked to date. The book will be of value as a reference to both clinicians and research scientists. refs.; figs.; tabs

  11. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Stinis, Panos [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-08-07

    This is the final report for the work conducted at the University of Minnesota (during the period 12/01/12-09/18/14) by PI Panos Stinis as part of the "Collaboratory on Mathematics for Mesoscopic Modeling of Materials" (CM4). CM4 is a multi-institution DOE-funded project whose aim is to conduct basic and applied research in the emerging field of mesoscopic modeling of materials.

  12. Muon performance aspects and measurement of the inclusive ZZ production cross section through the four lepton final state with the ATLAS experiment at the LHC

    International Nuclear Information System (INIS)

    Meyer, Jochen

    2013-01-01

    . Thus a first central issue is the performance of the spectrometer in terms of physics objects that are recognized by the device, the compatibility of data and the existing simulation as well as its improvement and finally the extension of the acceptance region. Once the excellent behavior and comprehension of the muon spectrometer is demonstrated, a second part addresses one physics use case of reconstructed muons. The electroweak force is part of the Standard Model and causes the interaction of heavy electroweak gauge bosons with fermions as well as their self-interaction. In proton-proton collisions such gauge bosons are produced. However, they decay immediately into a pair of fermions. In case of the Z boson, which is one of the gauge bosons, oppositely charged fermions of the same generation, including muons, emerge. The various decay modes are determined precisely at particle accelerators other than the LHC. However, the associated production of two Z bosons is measured less exactly at those facilities because of a very low cross section. The corresponding results acquired with the ATLAS experiment exceed all previous measurements in terms of statistics and accuracy. They are reported in this thesis as obtained from the observation of events with four charged leptons. The enhancement of the signal yield based on the extension of the muon spectrometer acceptance is especially emphasized as well as alternative methods to estimate background events. Furthermore, the impact on the probing of couplings of three Z bosons and intersection with the search for the Standard Model Higgs boson are pointed out.

  13. Antibody Production with Synthetic Peptides.

    Science.gov (United States)

    Lee, Bao-Shiang; Huang, Jin-Sheng; Jayathilaka, Lasanthi P; Lee, Jenny; Gupta, Shalini

    2016-01-01

    Peptides (usually 10-20 amino acid residues in length) can be used as effectively as proteins in raising antibodies producing both polyclonal and monoclonal antibodies routinely with titers higher than 20,000. Peptide antigens do not function as immunogens unless they are conjugated to proteins. Production of high quality antipeptide antibodies is dependent upon peptide sequence selection, the success of peptide synthesis, peptide-carrier protein conjugation, the humoral immune response in the host animal, the adjuvant used, the peptide dose administered, the injection method, and the purification of the antibody. Peptide sequence selection is probably the most critical step in the production of antipeptide antibodies. Although the process for designing peptide antigens is not exact, several guidelines and computational B-cell epitope prediction methods can help maximize the likelihood of producing antipeptide antibodies that recognize the protein. Antibodies raised by peptides have become essential tools in life science research. Virtually all phospho-specific antibodies are now produced using phosphopeptides as antigens. Typically, 5-20 mg of peptide is enough for antipeptide antibody production. It takes 3 months to produce a polyclonal antipeptide antibody in rabbits that yields ~100 mL of serum which corresponds to ~8-10 mg of the specific antibody after affinity purification using a peptide column.

  14. 21 CFR 864.9175 - Automated blood grouping and antibody test system.

    Science.gov (United States)

    2010-04-01

    ...) Identification. An automated blood grouping and antibody test system is a device used to group erythrocytes (red blood cells) and to detect antibodies to blood group antigens. (b) Classification. Class II (performance... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Automated blood grouping and antibody test system...

  15. The significance of antiphospholipid antibodies in pregnant women with chronic hypertension

    NARCIS (Netherlands)

    Zeeman, G. G.; Alexander, JM; McIntire, DD; Leveno, KJ

    The objective of this study was to perform antiphospholipid antibody screening in women with chronic hypertension to assess whether the presence of such antibodies is associated with adverse pregnancy outcome. Serum for anticardiolipin antibodies and lupus anticoagulant was obtained in pregnant

  16. Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq.

    Science.gov (United States)

    Busby, Michele; Xue, Catherine; Li, Catherine; Farjoun, Yossi; Gienger, Elizabeth; Yofe, Ido; Gladden, Adrianne; Epstein, Charles B; Cornett, Evan M; Rothbart, Scott B; Nusbaum, Chad; Goren, Alon

    2016-01-01

    The robustness of ChIP-seq datasets is highly dependent upon the antibodies used. Currently, polyclonal antibodies are the standard despite several limitations: They are non-renewable, vary in performance between lots and need to be validated with each new lot. In contrast, monoclonal antibody lots are renewable and provide consistent performance. To increase ChIP-seq standardization, we investigated whether monoclonal antibodies could replace polyclonal antibodies. We compared monoclonal antibodies that target five key histone modifications (H3K4me1, H3K4me3, H3K9me3, H3K27ac and H3K27me3) to their polyclonal counterparts in both human and mouse cells. Overall performance was highly similar for four monoclonal/polyclonal pairs, including when we used two distinct lots of the same monoclonal antibody. In contrast, the binding patterns for H3K27ac differed substantially between polyclonal and monoclonal antibodies. However, this was most likely due to the distinct immunogen used rather than the clonality of the antibody. Altogether, we found that monoclonal antibodies as a class perform equivalently to polyclonal antibodies for the detection of histone post-translational modifications in both human and mouse. Accordingly, we recommend the use of monoclonal antibodies in ChIP-seq experiments.

  17. Radioimmunotherapy of Non-Hodgkin's Lymphoma. The interaction of radiation and antibody with lymphoma cells

    International Nuclear Information System (INIS)

    Illidge, T.M.

    1999-06-01

    Whilst many patients with indolent Non-Hodgkin's Lymphoma (NHL) can achieve clinical remissions to first-line chemotherapy and/or radiotherapy, most will relapse. Current treatment options for relapsing patients are limited since most patients become resistant to repeated chemotherapy. Death usually occurs within 10 years of diagnosis. Overall, these disappointing results have not changed significantly in a quarter of a century and clearly advocate the urgent priority to research into potential new therapeutic approaches into this diverse and increasingly prevalent group of human tumours. Radioimmunotherapy (RIT) is currently under investigation as a new approach for the treatment of this disease. In this form of treatment, radionuclide-labeled monoclonal antibodies are able to deliver selective systemic irradiation by recognising tumour-associated antigens. The use of RIT with radiolabeled anti-CD20 antibodies in patients with recurrent B-cell lymphoma has resulted in extremely high rates of durable complete remissions. The optimal approach and mechanisms of action of successful RIT remain however largely unknown. The work described in this thesis has focused on clarifying some of the important determinants and mechanisms of effective RIT of syngeneic B-cell lymphoma, both in vivo and in vitro. A successful animal model of RIT in B cell lymphomas was established by initially generating a panel of antibodies against mouse B cell antigens. The in vitro characteristics of these antibodies have been compared with their subsequent performance, in biodistribution studies and RIT in vivo. For the first time in an in vivo model the relative contributions of antibody and irradiation are described. Some antibodies including anti-MHC Class II were shown to be effective delivery vehicles of low doses of Iodine-131. These antibodies, which appear to be inactive delivery vehicles can cure animals with low burdens of tumour. However antibodies such as anti-idiotype and anti-CD40

  18. SBIR PHASE I FINAL REPORT: Adoption of High Performance Computational (HPC) Modeling Software for Widespread Use in the Manufacture of Welded Structures

    Energy Technology Data Exchange (ETDEWEB)

    Brust, Frederick W. [Engineering Mechanics Corporation of Columbus (Emc2), Columbus, OH (United States); Punch, Edward F. [Engineering Mechanics Corporation of Columbus (Emc2), Columbus, OH (United States); Kurth, Elizabeth A. [Engineering Mechanics Corporation of Columbus (Emc2), Columbus, OH (United States); Kennedy, James C. [Engineering Mechanics Corporation of Columbus (Emc2), Columbus, OH (United States)

    2013-12-02

    element modeling. Finally, a plan for porting VFT onto the Ohio Supercomputer Center (OSC) through its hosted Manufacturing and Polymer Portal has been developed. This access route will permit SMEs to perform weld modeling to improve their competitiveness at a reasonable cost. All of these improvements are detailed in this repo

  19. Novel exons and splice variants in the human antibody heavy chain identified by single cell and single molecule sequencing.

    Directory of Open Access Journals (Sweden)

    Christopher Vollmers

    Full Text Available Antibody heavy chains contain a variable and a constant region. The constant region of the antibody heavy chain is encoded by multiple groups of exons which define the isotype and therefore many functional characteristics of the antibody. We performed both single B cell RNAseq and long read single molecule sequencing of antibody heavy chain transcripts and were able to identify novel exons for IGHA1 and IGHA2 as well as novel isoforms for IGHM antibody heavy chain.

  20. [Study of anti-idiotype antibodies to human monoclonal antibody].

    Science.gov (United States)

    Harada, R; Takahashi, N; Owaki, I; Kannagi, R; Endo, N; Morita, N; Inoue, M

    1992-02-01

    A human monoclonal antibody, ll-50 (IgM, lambda), was generated, which reacted specifically with a major of glycolipid present in LS174T colon cancer cells. The glycolipid antigen which reacted with the ll-50 antibody was expected to four sugar residues from its TLC mobility, and it was ascertained that the glycolipid antigen which reacted with ll-50 antibody might be Lc4 antigen [Gal beta 1----3 GLcNAc beta 1----3 Gal beta 1----4 Glc beta 1----1 Cer] judging from TLC immunostaining and ELISA when the reactivity of ll-50 antibody was tested using various pure glycolipids in 3-5 sugar residues as an antigen. Sera in patients with malignant disorders and healthy individuals were analyzed by Sandwich assay of immobilized and biotinylated ll-50 antibody. The serum of the Lc4 antigen recognized by ll-50 antibody was significantly higher in patients with malignant disorders than that in healthy individuals (p less than 0.05). Three mouse monoclonal anti-idiotype antibodies, G3, B3 and C5 (all IgG1), were generated by the immunization of BALB/c mice with ll-50 antibody. These anti-idiotype antibodies specifically bound to to human monoclonal antibody, ll-50 and had a significant inhibitory activity towards the binding of ll-50 antibody to the Lc4 antigen. This indicated that these anti-idiotype antibodies, G3, B3, and C5, were paratope-related anti-idiotype antibodies. G3, B3, and C5 were expected to define the nearest idiotope because they could mutually inhibit ll-50 antibody. Sera in patients with malignant disorders and healthy individuals were analyzed by Sandwich assay of immobilized and biotinylated anti-idiotype antibodies, G3, B3, and C5. As to the ll-50 like antibodies defined by C5 (Id-C5+), the mean serum level in patients with malignant disorders was significantly higher than that in healthy individuals (p less than 0.05). As to the ll-50 like antibodies defined by B3 (Id-B3+), the mean serum level in patients with malignant disorders was significantly higher

  1. Conclusiones finales

    OpenAIRE

    Guerrero Gaitán, Manuel

    2016-01-01

    La investigación realizada permite extraer las siguientes conclusiones finales que serán agrupadas según los principales problemas abordados: 1. En relación a las cláusulas que impiden una adecuada transferencia de tecnología, en la presente investigación se demuestra: Primero. Que las cláusulas más frecuentes recogidas en los contratos internacionales de transferencia de tecnología son: la fijación de precios, las restricciones a la investigación y adaptación de la tecnología objeto del cont...

  2. Prenatal toxoplasmosis antibody and childhood autism.

    Science.gov (United States)

    Spann, Marisa N; Sourander, Andre; Surcel, Heljä-Marja; Hinkka-Yli-Salomäki, Susanna; Brown, Alan S

    2017-05-01

    There is evidence that some maternal infections during the prenatal period are associated with neurodevelopmental disorders, such as childhood autism. However, the association between autism and Toxoplasma gondii (T. gondii), an intracellular parasite, remains unclear. The authors examined whether serologically confirmed maternal antibodies to T. gondii are associated with odds of childhood autism in offspring. The study is based on a nested case-control design of a large national birth cohort (N = 1.2 million) and the national psychiatric registries in Finland. There were 874 cases of childhood autism and controls matched 1:1 on date of birth, sex, birthplace and residence in Finland. Maternal sera were prospectively assayed from a national biobank for T. gondii IgM and IgG antibodies; IgG avidity analyses were also performed. High maternal T. gondii IgM antibody was associated with a significantly decreased odds of childhood autism. Low maternal T. gondii IgG antibody was associated with increased offspring odds of autism. In women with high T. gondii IgM antibodies, the IgG avidity was high for both cases and controls, with the exception of three controls. The findings suggest that the relationship between maternal T. gondii antibodies and odds of childhood autism may be related to the immune response to this pathogen or the overall activation of the immune system. Autism Res 2017, 10: 769-777. © 2016 International Society for Autism Research, Wiley Periodicals, Inc. © 2016 International Society for Autism Research, Wiley Periodicals, Inc.

  3. Human antibody production in transgenic animals.

    Science.gov (United States)

    Brüggemann, Marianne; Osborn, Michael J; Ma, Biao; Hayre, Jasvinder; Avis, Suzanne; Lundstrom, Brian; Buelow, Roland

    2015-04-01

    Fully human antibodies from transgenic animals account for an increasing number of new therapeutics. After immunization, diverse human monoclonal antibodies of high affinity can be obtained from transgenic rodents, while large animals, such as transchromosomic cattle, have produced respectable amounts of specific human immunoglobulin (Ig) in serum. Several strategies to derive animals expressing human antibody repertoires have been successful. In rodents, gene loci on bacterial artificial chromosomes or yeast artificial chromosomes were integrated by oocyte microinjection or transfection of embryonic stem (ES) cells, while ruminants were derived from manipulated fibroblasts with integrated human chromosome fragments or human artificial chromosomes. In all strains, the endogenous Ig loci have been silenced by gene targeting, either in ES or fibroblast cells, or by zinc finger technology via DNA microinjection; this was essential for optimal production. However, comparisons showed that fully human antibodies were not as efficiently produced as wild-type Ig. This suboptimal performance, with respect to immune response and antibody yield, was attributed to imperfect interaction of the human constant region with endogenous signaling components such as the Igα/β in mouse, rat or cattle. Significant improvements were obtained when the human V-region genes were linked to the endogenous CH-region, either on large constructs or, separately, by site-specific integration, which could also silence the endogenous Ig locus by gene replacement or inversion. In animals with knocked-out endogenous Ig loci and integrated large IgH loci, containing many human Vs, all D and all J segments linked to endogenous C genes, highly diverse human antibody production similar to normal animals was obtained.

  4. Oral lichen planus with antibodies to desmogleins 1 and 3.

    Science.gov (United States)

    Kinjyo, Chihiro; Kaneko, Takahide; Korekawa, Ayumi; Rokunohe, Akiko; Aizu, Takayuki; Matsuzaki, Yasushi; Nakano, Hajime; Sawamura, Daisuke

    2015-01-01

    Lichen planus (LP) is a chronic inflammatory disorder involving the skin or mucous membranes. Previous studies have demonstrated that some LP patients showed positive enzyme-linked immunosorbent assay (ELISA) for desmoglein (DSG) antibodies. We report a case with intractable painful oral lesions. ELISA indices for DSG1 and 3 antibodies were increased by 49 and 36, respectively. Histopathological analysis revealed irregular acanthosis and band-like infiltration of lymphocytes at the dermal-epidermal interface. Direct immunofluorescence revealed negative deposits of immunoglobulin G and C3 in intracellular spaces of the epidermis. Indirect immunofluorescence of normal skin also did not detect any antibodies. Consequently, we made a final diagnosis of oral LP. The previous two LP cases with positive ELISA for DSG antibodies and our case manifested the erosive form, the most advanced oral LP. Therefore, it is a possibility that severe damage of keratinocytes may induce generation of DSG antibodies. However, negative results of immunofluorescence and no relation between disease severity and titers of antibodies make the possibility unlikely. We should measure titers of DSG antibodies in LP patients and accumulate data to establish a valid conclusion. © 2014 Japanese Dermatological Association.

  5. Antibody Maturation in Trypanosoma cruzi-Infected Rats

    Science.gov (United States)

    Marcipar, Iván S.; Risso, Marikena G.; Silber, Ariel M.; Revelli, Silvia; Marcipar, Alberto J.

    2001-01-01

    The study of antibody avidity changes during infection has improved the understanding of the pathologic processes involved in several infectious diseases. In some infections, like toxoplasmosis, this information is being used for diagnostic purposes. Results of the evolution of antibody avidity for different specific antigens in Trypanosome cruzi-infected rats are presented. A Western blotting technique, combined with avidity analysis to identify antigens that elicit high-avidity antibodies, is suggested. In this system, antibodies showed high avidity values only during the chronic phase of infection and only in relation to antibodies against 21-, 33-, 41-, 42-, 56-, 58-, 66-, and 72-kDa antigens. Finally, a 97-kDa T. cruzi antigen, which was recognized by high-avidity antibodies and occurred in noninfected rats, was identified. These results allow us to evaluate the different antigens in chagasic infection. Our results show that with the correct choice of antigen it is possible to detect differences in maturation of antibodies and to discriminate, in an experimental model, between recent (acute) and chronic infections. PMID:11427430

  6. Longitudinal study of interferon-gamma, serum antibody and milk antibody responses in cattle infected with Mycobacterium avium subsp paratuberculosis

    DEFF Research Database (Denmark)

    Huda, A.; Jungersen, Gregers; Lind, Peter

    2004-01-01

    -blood lymphocytes (IFN-gamma test), and measurement of antibody responses against M. paratuberculosis in serum and milk by an in-house absorbed ELISA. The IFN-gamma test diagnosed higher proportions of infected and exposed animals than the antibody ELISAs. The highest sensitivity of IFN-gamma test was in infected...... cattle of 2+ years of age. Receiver-operating characteristic (ROC) analyses supported the assumption that the IFN-gamma test had a better performance than antibody tests of animals of 1+ and 2+ years of age. However, for animals of 3+ years all tests performed equally well. Application of single sampling...... compared with repeated samplings showed better performance of the IFN-gamma test by repeated samplings, and the milk antibody ELISA in animals of 3+ years of age performed significantly better with repeated sampling compared with single sampling. In conclusion, the IFN-gamma test may be applied...

  7. The antibody Hijikata Tatsumi

    Directory of Open Access Journals (Sweden)

    Éden Peretta

    2012-11-01

    Full Text Available Considered one of the most influential modern dance representatives in Japan, Tatsumi Hijikata’s work was a milestone in the Japanese post-war experimental artistic scene. Heretic son of his time, he staged a fertile mix of artistic and cultural influences, overlapping subversive elements of European arts and philosophy with radical references from pre-modern Japanese culture. In this way he built the foundations of its unstable antibody, its political-artistic project of dissolution of a organism, both physical and social.

  8. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Jarillo-Herrero, Pablo [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2017-02-07

    This is the final report of our research program on electronic transport experiments on Topological Insulator (TI) devices, funded by the DOE Office of Basic Energy Sciences. TI-based electronic devices are attractive as platforms for spintronic applications, and for detection of emergent properties such as Majorana excitations , electron-hole condensates , and the topological magneto-electric effect . Most theoretical proposals envision geometries consisting of a planar TI device integrated with materials of distinctly different physical phases (such as ferromagnets and superconductors). Experimental realization of physics tied to the surface states is a challenge due to the ubiquitous presence of bulk carriers in most TI compounds as well as degradation during device fabrication.

  9. The future of monoclonal antibody technology

    OpenAIRE

    Zider, Alexander; Drakeman, Donald L

    2010-01-01

    With the rapid growth of monoclonal antibody-based products, new technologies have emerged for creating modified forms of antibodies, including fragments, conjugates and multi-specific antibodies. We created a database of 450 therapeutic antibodies in development to determine which technologies and indications will constitute the “next generation” of antibody products. We conclude that the antibodies of the future will closely resemble the antibodies that have already been approved for commer...

  10. Monoclonal antibodies for treating cancer

    International Nuclear Information System (INIS)

    Dillman, R.O.

    1989-01-01

    The purpose of this study is to assess the current status of in-vivo use of monoclonal antibodies for treating cancer. Publications appearing between 1980 and 1988 were identified by computer searches using MEDLINE and CANCERLIT, by reviewing the table of contents of recently published journals, and by searching bibliographies of identified books and articles. More than 700 articles, including peer-reviewed articles and book chapters, were identified and selected for analysis. The literature was reviewed and 235 articles were selected as relevant and representative of the current issues and future applications for in-vivo monoclonal antibodies for cancer therapy and of the toxicity and efficacy which has been associated with clinical trials. Approaches include using antibody alone (interacting with complement or effector cells or binding directly with certain cell receptors) and immunoconjugates (antibody coupled to radioisotopes, drugs, toxins, or other biologicals). Most experience has been with murine antibodies. Trials of antibody alone and radiolabeled antibodies have confirmed the feasibility of this approach and the in-vivo trafficking of antibodies to tumor cells. However, tumor cell heterogeneity, lack of cytotoxicity, and the development of human antimouse antibodies have limited clinical efficacy. Although the immunoconjugates are very promising, heterogeneity and the antimouse immune response have hampered this approach as has the additional challenge of chemically or genetically coupling antibody to cytotoxic agents. As a therapeutic modality, monoclonal antibodies are still promising but their general use will be delayed for several years. New approaches using human antibodies and reducing the human antiglobulin response should facilitate treatment. 235 references

  11. Males without apparent alloimmunization could have HLA antibodies that recognize target HLA specificities expressed on cells.

    Science.gov (United States)

    Nakamura, J; Nakajima, F; Kamada, H; Tadokoro, K; Nagai, T; Satake, M

    2017-05-01

    Human leukocyte antigen (HLA) antibodies, which are involved in the development of transfusion-related side effects such as transfusion-related lung injury, are sometimes found in males without a history of alloimmunization (eg, transplantation and transfusion). Whether HLA antibodies in male donors can interact with their target HLA specificities expressed on cells have not been completely investigated. The HLA antibodies detected in 7 male donors were characterized. Flow cytometry and immunocomplex capture fluorescence analysis were performed to evaluate the ability of these antibodies to bind with target HLA specificities expressed on cells. The association of these antibodies with complement was examined using anti-C1q antibody. Sustainability of HLA antibodies over time was compared in 26 male vs 57 female donors. The antibodies from all 7 donors recognized intact HLA molecules coated onto microbeads. The antibodies in 2 of 7 donors also recognized their target HLA specificities expressed on cells. Furthermore, the antibodies in one of these 2 donors showed HLA specificities that involved complement binding. Twenty-one of 26 initially positive male donors had turned negative for HLA antibody at least 1 year after their initial positive screening, whereas HLA antibody positivity was maintained for a long time in most female donors. Males without apparent alloimmunization could have HLA antibodies that recognize their target HLA specificities on cells and that could potentially modify molecular events in affected cells. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  12. Tabhu: tools for antibody humanization

    DEFF Research Database (Denmark)

    Olimpieri, Pier Paolo; Marcatili, Paolo; Tramontano, Anna

    2015-01-01

    and time-consuming experiments. Here we present tools for antibody humanization (Tabhu) a web server for antibody humanization. Tabhu includes tools for human template selection, grafting, back-mutation evaluation, antibody modelling and structural analysis, helping the user in all the critical steps...... elicit unwanted and dangerous immunogenic responses. Antibody humanization methods are designed to produce molecules with a better safety profile still maintaining their ability to bind the antigen. This can be accomplished by grafting the non-human regions determining the antigen specificity...... of the humanization experiment protocol....

  13. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Hollingsworth, Jeff

    2014-04-04

    The goal of this project was to create a community tool infrastructure for program development tools targeting Petascale class machines and beyond. This includes performance analysis, debugging, and correctness tools, as well as tuning and optimization frameworks. The infrastructure provides a comprehensive and extensible set of individual tool building components. Within this project we developed the basis for this infrastructure as well as set of core modules that allow a comprehensive performance analysis at scale. Further, we developed a methodology and workflow that allows others to add or replace modules, to integrate parts into their own tools, or to customize existing solutions.

  14. Universal influenza virus vaccines and therapeutic antibodies.

    Science.gov (United States)

    Nachbagauer, R; Krammer, F

    2017-04-01

    Current influenza virus vaccines are effective when well matched to the circulating strains. Unfortunately, antigenic drift and the high diversity of potential emerging zoonotic and pandemic viruses make it difficult to select the right strains for vaccine production. This problem causes vaccine mismatches, which lead to sharp drops in vaccine effectiveness and long response times to manufacture matched vaccines in case of novel pandemic viruses. To provide an overview of universal influenza virus vaccines and therapeutic antibodies in preclinical and clinical development. PubMed and clinicaltrials.gov were used as sources for this review. Universal influenza virus vaccines that target conserved regions of the influenza virus including the haemagglutinin stalk domain, the ectodomain of the M2 ion channel or the internal matrix and nucleoproteins are in late preclinical and clinical development. These vaccines could confer broad protection against all influenza A and B viruses including drift variants and thereby abolish the need for annual re-formulation and re-administration of influenza virus vaccines. In addition, these novel vaccines would enhance preparedness against emerging influenza virus pandemics. Finally, novel therapeutic antibodies against the same conserved targets are in clinical development and could become valuable tools in the fight against influenza virus infection. Both universal influenza virus vaccines and therapeutic antibodies are potential future options for the control of human influenza infections. Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  15. Theranostics Using Antibodies and Antibody-Related Therapeutics

    NARCIS (Netherlands)

    Moek, Kirsten L; Giesen, Danique; Kok, Iris C; de Groot, Derk Jan A; Jalving, Mathilde; Fehrmann, Rudolf S N; Lub-de Hooge, Marjolijn N; Brouwers, Adrienne H; de Vries, Elisabeth G E

    In theranostics, radiolabeled compounds are used to determine a treatment strategy by combining therapeutics and diagnostics in the same agent. Monoclonal antibodies (mAbs) and antibody-related therapeutics represent a rapidly expanding group of cancer medicines. Theranostic approaches using these

  16. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Webb, Robert C. [Texas A& M University; Kamon, Teruki [Texas A& M University; Toback, David [Texas A& M University; Safonov, Alexei [Texas A& M University; Dutta, Bhaskar [Texas A& M University; Dimitri, Nanopoulos [Texas A& M University; Pope, Christopher [Texas A& M University; White, James [Texas A& M University

    2013-11-18

    Overview The High Energy Physics Group at Texas A&M University is submitting this final report for our grant number DE-FG02-95ER40917. This grant has supported our wide range of research activities for over a decade. The reports contained here summarize the latest work done by our research team. Task A (Collider Physics Program): CMS & CDF Profs. T. Kamon, A. Safonov, and D. Toback co-lead the Texas A&M (TAMU) collider program focusing on CDF and CMS experiments. Task D: Particle Physics Theory Our particle physics theory task is the combined effort of Profs. B. Dutta, D. Nanopoulos, and C. Pope. Task E (Underground Physics): LUX & NEXT Profs. R. Webb and J. White(deceased) lead the Xenon-based underground research program consisting of two main thrusts: the first, participation in the LUX two-phase xenon dark matter search experiment and the second, detector R&D primarily aimed at developing future detectors for underground physics (e.g. NEXT and LZ).

  17. Interference of daratumumab with pretransfusion testing, mimicking a high-titer, low avidity like antibody

    Directory of Open Access Journals (Sweden)

    Mei-Hwa Lin

    2017-01-01

    Full Text Available Daratumumab is a monoclonal immunoglobulin against CD38 and has been approved for treating patients with refractory multiple myeloma. The presence of daratumumab in the sera can interfere with pretransfusion testing due to the weakly expression of CD38 on red cells. The reactivity could be mistaken as autoantibody (if autocontrol is positive or alloantibody (if autocontrol is negative. We present a case that demonstrates daratumumab could mimic a high titer low avidity (HTLA alloantibody. A 34-year-old male patient of refractory myeloma was recruited in phase three clinical trial involving daratumumab. Samples were sent to the blood bank for pretransfusion testing. Without knowledge of patient having used daratumumab, we mistook the reactivity in the patient's sera as an HTLA antibody due to the results of negative autocontrol and high titers of antibody activity. Antibody screen showed a panreactive pattern and the reactivity against screening cells was up to a titer of 1: 1240. The reactivity was weaker against cord cells than adult cells, became weaker against ZZAP-treated cells and became negative against DDT-treated cells. A discussion with attending physician finally revealed the reactivity was due to the interference caused by daratumumab. The case demonstrates good communication is essential in performing pretransfusion testing for patients receiving daratumumab and other new biological regimens that can interfere with compatibility test.

  18. Interference of daratumumab with pretransfusion testing, mimicking a high-titer, low avidity like antibody.

    Science.gov (United States)

    Lin, Mei-Hwa; Liu, Fei-Yun; Wang, Hsiu-Mien; Cho, Hsin-Ching; Lo, Shyh-Chyi

    2017-01-01

    Daratumumab is a monoclonal immunoglobulin against CD38 and has been approved for treating patients with refractory multiple myeloma. The presence of daratumumab in the sera can interfere with pretransfusion testing due to the weakly expression of CD38 on red cells. The reactivity could be mistaken as autoantibody (if autocontrol is positive) or alloantibody (if autocontrol is negative). We present a case that demonstrates daratumumab could mimic a high titer low avidity (HTLA) alloantibody. A 34-year-old male patient of refractory myeloma was recruited in phase three clinical trial involving daratumumab. Samples were sent to the blood bank for pretransfusion testing. Without knowledge of patient having used daratumumab, we mistook the reactivity in the patient's sera as an HTLA antibody due to the results of negative autocontrol and high titers of antibody activity. Antibody screen showed a panreactive pattern and the reactivity against screening cells was up to a titer of 1: 1240. The reactivity was weaker against cord cells than adult cells, became weaker against ZZAP-treated cells and became negative against DDT-treated cells. A discussion with attending physician finally revealed the reactivity was due to the interference caused by daratumumab. The case demonstrates good communication is essential in performing pretransfusion testing for patients receiving daratumumab and other new biological regimens that can interfere with compatibility test.

  19. Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins

    Directory of Open Access Journals (Sweden)

    Ângela L. Cabana

    2015-06-01

    Full Text Available Abstract: This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies in captive penguins by double radial agar gel immunodiffusion (AGID for the aspergillosis diagnosis. We included 134 Magellanic penguins (Spheniscus magellanicus in rehabilitation at the Center for Recovery of Marine Animals (CRAM / FURG. All of them were monitored by AGID weekly until its final destination (death or release, totalizing 660 serum samples studied. All animals were clinically accompanied and post-mortem examinations was performed in penguins that died during the studied period. A total of 28% (37/134 of the penguins died, 89.2% (33/37 due to aspergillosis, 11% (4/37 by other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 presented anti- A. fumigatus antibodies by AGID, being the average interval between death and positive AGID 16.4 days. Twelve animals with negative serology died of aspergillosis. The sensitivity and specificity rates were 63.6% and 95% respectively, and the positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that the serological monitoring for detection of antibodies by AGID can be an important tool for the diagnosis of aspergillosis in penguins.

  20. Serial plasmapheresis in a haemophiliac with antibodies to FVIII.

    Science.gov (United States)

    Cobcroft, R; Tamagnini, G; Dormandy, K M

    1977-08-01

    Serial plasmaphereses were performed on a 23-year-old haemophiliac, with antibodies to factor VIII (FVIII), in order to reduce the antibody level before multiple dental extractions. Eight phereses were carried out in which approximately 1.5 litres plasma was exchanged with isotonic saline. By the ninth exchange, which was carried out immediately before the extraction, the antibody level had fallen from 4 u/ml to 0.8 u/ml. On this occasion fresh frozen plasma and FVIII concentrate were infused immediately after the pheresis. A normal post-infusion level of FVIII was achieved with a normal half disappearance time of the infused FVIII.

  1. Study on the technical parameters of two different systems of RIA performed with solid-phase antibody test tubes prepared with magnetic microparticle covalence conjagation or conventional physical absorption

    International Nuclear Information System (INIS)

    Chen Zhiqiang; Wang Chengmin; Tang Baojun

    2007-01-01

    Objective: To investigate a new method of preparation of solid-phase antibody with flurorescein isothioeynate (FITC)-anti FITC magnetic nanoparticles system (for FT 3 and TSH). Methods: FT 3 and TSH monoclonal antibody IgC was la- belied with FITC. Anti-FITC magnetic mieroparticles was prepared and conjugated with the FITC labelled antibody to form the solid - phase coated test tube for RIA. Solid-phase test tube prepared with the conventional physical absorption method was also used for RIA and the technical parameters of the two systems were compared. Results: For FT 3 , the sensitivity was 0.18pmol/L with the new method and 0.43pmol/L with the conventional method. Other parameters were; intra-CV, 8.96% vs 16.26%; inter-CV, 15.25% vs 18.83%, correlation with PR method r=0.9825 vs r=0.9102. For TSH, sensitivity was 0.061 μIU/ml vs 0.04 μ IU/ml, intra- CV, 7.6% vs 6.92%, inter-CV, 8.55% vs 14.23%, correlation between the new and conventional method, r=0. 9987. TSH RIA was especially rapid with the new technic: 79 min vs 190 min. Conclusion: For FT 3 and TSH RIA, the new method takes much less time with increased homogeniety. (authors)

  2. Performance and Economics of Catalytic Glow Plugs and Shields in Direct Injection Natural Gas Engines for the Next Generation Natural Gas Vehicle Program: Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Mello, J. P.; Bezaire, D.; Sriramulu, S.; Weber, R.

    2003-08-01

    Subcontractor report details work done by TIAX and Westport to test and perform cost analysis for catalytic glow plugs and shields for direct-injection natural gas engines for the Next Generation Natural Gas Vehicle Program.

  3. Arsenic Removal from Drinking Water by Adsorptive Media U.S. EPA Demonstration Project at Richmond Elementary School in Susanville, CA Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed and the results obtained for the arsenic removal treatment technology demonstration project at Richmond Elementary School in Susanville, CA. The objectives of the project were to evaluate: (1) the effectiveness of an Aquatic Treatme...

  4. Medical Student Dissection of CadaversImproves Performance on Practical Exams, but not Dissection-Relevant Questions in the NBME Gross Anatomy and Embryology Final Exam

    Directory of Open Access Journals (Sweden)

    Leslie Sargent Jones

    2001-03-01

    Full Text Available We have examined whether cadaver dissection by first year medical students (MIs affected their performance in two test measures: the NBME Gross Anatomy and Embryology Subject Exam (dissection-relevant questions only, and practical exams given at the end of each major section within the course. The dissections for the entire course were divided into 18 regional dissection units and each student was assigned to dissect one third of the regional units; the other two-thirds of the material was learned from the partner-prosected cadavers. Performance for each student on the exams was then assessed as a function of the regions those students actually dissected. While the results indicated a small performance advantage for MIs answering questions on material they had dissected on the NBME Subject Exam questions relevant to dissection (78-88% of total exam, the results were not statistically significant. However, a similar, small performance advantage on the course practical exams was highly significant.

  5. Transportation cost index : a comprehensive performance measure for transportation and land and its application in OR, FL, and UT : final report.

    Science.gov (United States)

    2017-04-01

    MAP-21 and state laws are placing increasing emphasis on using comprehensive transportation performance measures that include mobility, : safety, economy, livability, equity, and environmental to guide transportation decision-making. One of the tough...

  6. PERFORMANCE

    Directory of Open Access Journals (Sweden)

    M Cilli

    2014-10-01

    Full Text Available This study aimed to investigate the kinematic and kinetic changes when resistance is applied in horizontal and vertical directions, produced by using different percentages of body weight, caused by jumping movements during a dynamic warm-up. The group of subjects consisted of 35 voluntary male athletes (19 basketball and 16 volleyball players; age: 23.4 ± 1.4 years, training experience: 9.6 ± 2.7 years; height: 177.2 ± 5.7 cm, body weight: 69.9 ± 6.9 kg studying Physical Education, who had a jump training background and who were training for 2 hours, on 4 days in a week. A dynamic warm-up protocol containing seven specific resistance movements with specific resistance corresponding to different percentages of body weight (2%, 4%, 6%, 8%, 10% was applied randomly on non consecutive days. Effects of different warm-up protocols were assessed by pre-/post- exercise changes in jump height in the countermovement jump (CMJ and the squat jump (SJ measured using a force platform and changes in hip and knee joint angles at the end of the eccentric phase measured using a video camera. A significant increase in jump height was observed in the dynamic resistance warm-up conducted with different percentages of body weight (p 0.05. In jump movements before and after the warm-up, while no significant difference between the vertical ground reaction forces applied by athletes was observed (p>0.05, in some cases of resistance, a significant reduction was observed in hip and knee joint angles (p<0.05. The dynamic resistance warm-up method was found to cause changes in the kinematics of jumping movements, as well as an increase in jump height values. As a result, dynamic warm-up exercises could be applicable in cases of resistance corresponding to 6-10% of body weight applied in horizontal and vertical directions in order to increase the jump performance acutely.

  7. Study of conjugation and radiolabeling of monoclonal antibody rituximab for use in radionuclide therapy

    International Nuclear Information System (INIS)

    Massicano, Adriana Vidal Fernandes

    2011-01-01

    Lymphomas are tumors originated from the transformation of a lymphocyte in the lymphatic system. The most common lymphoma is the Non-Hodgkin Lymphoma (NHL). Advances in immunology and molecular biology have been improving NHL's detection and treatment strategies development, such as Radioimmunotherapy (RIT). Rituximab is an anti-CD20 monoclonal antibody used as immunotherapeutic to treat refractory or relapsed NHL. The goal of the present work was to conjugate this antibody to DOTA-NHS-ester bifunctional chelator and to radiolabel it with 177 Lu radioisotope in order to develop a radio immunotherapeutic agent for NHL's treatment. Different rituximab to DOTA molar ratios (1:5, 1:10, 1:20, 1:50, 1:250, 1:500 and 1:1000) were evaluated in order to determine the best condition for obtaining the highest radiochemical purity of radio immunotherapeutic. The stability of the unlabeled immuno conjugated was evaluated by high performance liquid chromatography (HPLC) for up to 240 days in different storage conditions. The stability of the labeled preparations was evaluated either after storing at 2-8 degree C or incubation in human serum at 37 degree C. The binding to serum proteins was also determined. In vivo studies were performed in healthy Swiss mice, in order to characterize the biological properties of labeled conjugate. Finally, preliminary studies of radio immuno conjugated competitive binding to CD20 positive Raji cells were carried out in order to analyze if the process of conjugation and radiolabeling compromises the immunoreactivity of the antibody. The conjugation applying lower antibody to chelator molar ratios (1:5, 1:10 and 1:20) showed high stability when stored for up to 240 days in different conditions. The HPLC analysis showed that the monoclonal antibody conjugated in molar ratio 1:50 was labeled with higher radiochemical purity (> 95%) when purified in PD-10 column. This conjugate showed reasonable stability at 2-8 degree C. The analysis of the

  8. Schedulability Analysis for Java Finalizers

    DEFF Research Database (Denmark)

    Bøgholm, Thomas; Hansen, Rene Rydhof; Søndergaard, Hans

    2010-01-01

    Java finalizers perform clean-up and finalisation of objects at garbage collection time. In real-time Java profiles the use of finalizers is either discouraged (RTSJ, Ravenscar Java) or even disallowed (JSR-302), mainly because of the unpredictability of finalizers and in particular their impact...... on the schedulability analysis. In this paper we show that a controlled scoped memory model results in a structured and predictable execution of finalizers, more reminiscent of C++ destructors than Java finalizers. Furthermore, we incorporate finalizers into a (conservative) schedulability analysis for Predictable Java...... programs. Finally, we extend the SARTS tool for automated schedulability analysis of Java bytecode programs to handle finalizers in a fully automated way....

  9. MATERIAL AND PROCESS DEVELOPMENT LEADING TO ECONOMICAL HIGH-PERFORMANCE THIN-FILM SOLID OXIDE FUEL CELLS. Final Technical Report (October 2000 - December 2003)

    International Nuclear Information System (INIS)

    Jie Guan; Nguyen Minh

    2003-01-01

    This report summarizes the results of the work conducted under the program: ''Material and Process Development Leading to Economical High-Performance Thin-Film Solid Oxide Fuel Cells'' under contract number DE-AC26-00NT40711. The program goal is to advance materials and processes that can be used to produce economical, high-performance solid oxide fuel cells (SOFC) capable of achieving extraordinary high power densities at reduced temperatures. Under this program, anode-supported thin electrolyte based on lanthanum gallate (LSMGF) has been developed using tape-calendering process. The fabrication parameters such as raw materials characteristics, tape formulations and sintering conditions have been evaluated. Dense anode supported LSGMF electrolytes with thickness range of 10-50 micron have been fabricated. High performance cathode based on Sr 0.5 Sm 0.5 CoO 3 (SSC) has been developed. Polarization of ∼0.23 ohm-cm 2 has been achieved at 600 C with Sr 0.5 Sm 0.5 CoO 3 cathode. The high-performance SSC cathode and thin gallate electrolyte have been integrated into single cells and cell performance has been characterized. Tested cells to date generally showed low performance because of low cell OCVs and material interactions between NiO in the anode and lanthanum gallate electrolyte

  10. Probable C4d-negative accelerated acute antibody-mediated rejection due to non-HLA antibodies.

    Science.gov (United States)

    Niikura, Takahito; Yamamoto, Izumi; Nakada, Yasuyuki; Kamejima, Sahoko; Katsumata, Haruki; Yamakawa, Takafumi; Furuya, Maiko; Mafune, Aki; Kobayashi, Akimitsu; Tanno, Yudo; Miki, Jun; Yamada, Hiroki; Ohkido, Ichiro; Tsuboi, Nobuo; Yamamoto, Hiroyasu; Yokoo, Takashi

    2015-07-01

    We report a case of probable C4d-negative accelerated acute antibody-mediated rejection due to non-HLA antibodies. A 44 year-old male was admitted to our hospital for a kidney transplant. The donor, his wife, was an ABO minor mismatch (blood type O to A) and had Gitelman syndrome. Graft function was delayed; his serum creatinine level was 10.1 mg/dL at 3 days after transplantation. Open biopsy was performed immediately; no venous thrombosis was observed during surgery. Histology revealed moderate peritubular capillaritis and mild glomerulitis without C4d immunoreactivity. Flow cytometric crossmatching was positive, but no panel-reactive antibodies against HLA or donor-specific antibodies (DSAbs) to major histocompatibility complex class I-related chain A (MICA) were detected. Taken together, we diagnosed him with probable C4d-negative accelerated antibody-mediated rejection due to non-HLA, non-MICA antibodies, the patient was treated with steroid pulse therapy (methylprednisolone 500 mg/day for 3 days), plasma exchange, intravenous immunoglobulin (40 g/body), and rituximab (200 mg/body) were performed. Biopsy at 58 days after transplantation, at which time S-Cr levels were 1.56 mg/dL, found no evidence of rejection. This case, presented with a review of relevant literature, demonstrates that probable C4d-negative accelerated acute AMR can result from non-HLA antibodies. © 2015 Asian Pacific Society of Nephrology.

  11. Antibodies and Plasmodium falciparum merozoites

    NARCIS (Netherlands)

    Ramasamy, R; Ramasamy, M; Yasawardena, S

    There is considerable interest in using merozoite proteins in a vaccine against falciparum malaria. Observations that antibodies to merozoite surface proteins block invasion are a basis for optimism. This article draws attention to important and varied aspects of how antibodies to Plasmodium

  12. Catalytic Antibodies: Concept and Promise

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 12; Issue 11. Catalytic Antibodies: Concept and Promise. Desirazu N Rao Bharath Wootla. General Article Volume 12 Issue ... Keywords. Catalytic antibodies; abzymes; hybridome technology; Diels– Alder reaction; Michaelis– Menten kinetics; Factor VIII.

  13. Antiphospholipid antibodies: standardization and testing.

    Science.gov (United States)

    Riley, R S; Friedline, J; Rogers, J S

    1997-09-01

    A phenomenon originally scorned as a laboratory nuisance has turned out to be an important cause of thromboembolism, fetal death, and other forms of human disease. Investigations of this inaptly named "lupus anticoagulant" has led to the discovery of at least two distinct types of autoimmune antibodies. In spite of recent discoveries regarding the pathophysiology of these antibodies, their clinical significance is still controversial.

  14. Educational paper: Primary antibody deficiencies

    NARCIS (Netherlands)

    G.J.A. Driessen (Gertjan); M. van der Burg (Mirjam)

    2011-01-01

    textabstractPrimary antibody deficiencies (PADs) are the most common primary immunodeficiencies and are characterized by a defect in the production of normal amounts of antigen-specific antibodies. PADs represent a heterogeneous spectrum of conditions, ranging from often asymptomatic selective IgA

  15. The Biotechnological Applications of Recombinant Single-Domain Antibodies are Optimized by the C-Terminal Fusion to the EPEA Sequence (C Tag

    Directory of Open Access Journals (Sweden)

    Selma Djender

    2014-04-01

    Full Text Available We designed a vector for the bacterial expression of recombinant antibodies fused to a double tag composed of 6xHis and the EPEA amino acid sequence. EPEA sequence (C tag is tightly bound by a commercial antibody when expressed at the C-term end of a polypeptide. The antigen is released in the presence of 2 M MgCl2. Consequently, constructs fused to the 6xHis-C tags can be purified by two successive and orthogonal affinity steps. Single-domain antibodies were produced either in the periplasmic or in the cytoplasmic space of E. coli. Surprisingly, the first affinity purification step performed using the EPEA-binding resin already yielded homogeneous proteins. The presence of the C tag did not interfere with the binding activity of the antibodies, as assessed by FACS and SPR analyses, and the C tag was extremely effective for immunoprecipitating HER2 receptor. Finally, the Alexa488-coupled anti-C tag allowed for simplification of FACS and IF analyses. These results show that a tag of minimal dimensions can be effectively used to improve the applicability of recombinant antibodies as reagents. In our hands, C tag was superior to His-tag in affinity purification and pull-down experiments, and practical in any other standard immune technique.

  16. [Antibody induction after intrauterine interventions].

    Science.gov (United States)

    Hoch, J; Giers, G; Bald, R; Hansmann, M; Hanfland, P

    1993-06-01

    Immunohematologic and clinical data, i.e., antibody profile, location of the placenta, mode of cordocentesis, obtained from 48 pregnant patients with irregular erythrocyte antibodies during the last 2 years have been retrospectively evaluated. All fetuses of the patients received intrauterine transfusions for the treatment of fetal erythroblastosis. In 16 (33%) patients (group I) a secondarily induced antibody was detected after the onset of intrauterine transfusion therapy. 32 (67%) patients (group II) did not further develop new antibody specificities. Group I exhibited a significantly different distribution in the location of the placenta (p pregnant women. In group I a 5-fold higher rate of anterior than posterior placenta location was found. The mode of cordocentesis differed significantly (p antibodies by invasive intrauterine interventions in our patients depended indirectly on the location of the placenta and directly on the mode of the puncture (trans- vs. paraplacental access).

  17. Research project implementation of a risk-based performance monitoring system for nuclear power plants: Phase II - Type-D indicators. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Sewell, R.T. [EQE International. Inc., Evergreen, CO (United States); Khatib-Rahbar, M. [Energy Research, Inc., Rockville, MD (United States); Erikson, H. [Swedish Nuclear Power Inspectorate, Stockholm (Sweden)

    1999-02-01

    This study has established the basis for incorporating a meaningful set of Type-D performance indicators into an overall performance monitoring system based on a PSA framework. The relationships developed as part of this study enable various organizational, maintenance, and operational influences, that are manifested through key events that can be identified and reported at a plant, to be accounted for in terms of their impacts on safety. The relationships and the capability they pose are significant new and unique developments. The relationships require that plant-specific data on the key performance indicators be acquired and analyzed. This, in turn, necessitates that a regular and systematic supplementary data collection program be implemented. Hence, it is recommended here that such a data program be developed and undertaken, specifically within the context of an overall PSA-based safety monitoring system. Plant licensees should be responsible for the supplemental data collection effort; however, the data collection requirements should not pose an undue burden on the licensees. To the extent possible, the data collection program should be coordinated, and possibly integrated, with existing licensee data collection and event reporting efforts. This study was undertaken to help verify whether or not it would be feasible to proceed with a PSA-based performance monitoring program. Research performed for Part-1 investigation has resulted in the development of a systematic and structured approach for monitoring safety performance based on all types of plant events. The Type-D relationships were earlier identified as a key element of the proposed performance monitoring system, and if it would be discovered that the Type-D relationships could not be practically developed and implemented, then it would have lead to the recognition that the performance monitoring system might have little practical value. On the other hand, if the Type-D relationships could be efficiently

  18. Research project implementation of a risk-based performance monitoring system for nuclear power plants: Phase II - Type-D indicators. Final report

    International Nuclear Information System (INIS)

    Sewell, R.T.; Khatib-Rahbar, M.; Erikson, H.

    1999-02-01

    This study has established the basis for incorporating a meaningful set of Type-D performance indicators into an overall performance monitoring system based on a PSA framework. The relationships developed as part of this study enable various organizational, maintenance, and operational influences, that are manifested through key events that can be identified and reported at a plant, to be accounted for in terms of their impacts on safety. The relationships and the capability they pose are significant new and unique developments. The relationships require that plant-specific data on the key performance indicators be acquired and analyzed. This, in turn, necessitates that a regular and systematic supplementary data collection program be implemented. Hence, it is recommended here that such a data program be developed and undertaken, specifically within the context of an overall PSA-based safety monitoring system. Plant licensees should be responsible for the supplemental data collection effort; however, the data collection requirements should not pose an undue burden on the licensees. To the extent possible, the data collection program should be coordinated, and possibly integrated, with existing licensee data collection and event reporting efforts. This study was undertaken to help verify whether or not it would be feasible to proceed with a PSA-based performance monitoring program. Research performed for Part-1 investigation has resulted in the development of a systematic and structured approach for monitoring safety performance based on all types of plant events. The Type-D relationships were earlier identified as a key element of the proposed performance monitoring system, and if it would be discovered that the Type-D relationships could not be practically developed and implemented, then it would have lead to the recognition that the performance monitoring system might have little practical value. On the other hand, if the Type-D relationships could be efficiently

  19. Relationship Between Final Performance and Block Times with the Traditional and the New Starting Platforms with A Back Plate in International Swimming Championship 50-M and 100-M Freestyle Events

    Science.gov (United States)

    Garcia-Hermoso, Antonio; Escalante, Yolanda; Arellano, Raul; Navarro, Fernando; Domínguez, Ana M.; Saavedra, Jose M.

    2013-01-01

    The purpose of this study was to investigate the association between block time and final performance for each sex in 50-m and 100-m individual freestyle, distinguishing between classification (1st to 3rd, 4th to 8th, 9th to 16th) and type of starting platform (old and new) in international competitions. Twenty-six international competitions covering a 13-year period (2000-2012) were analysed retrospectively. The data corresponded to a total of 1657 swimmers’ competition histories. A two-way ANOVA (sex x classification) was performed for each event and starting platform with the Bonferroni post-hoc test, and another two-way ANOVA for sex and starting platform (sex x starting platform). Pearson’s simple correlation coefficient was used to determine correlations between the block time and the final performance. Finally, a simple linear regression analysis was done between the final time and the block time for each sex and platform. The men had shorter starting block times than the women in both events and from both platforms. For 50-m event, medalists had shorter block times than semi- finalists with the old starting platforms. Block times were directly related to performance with the old starting platforms. With the new starting platforms, however, the relationship was inverse, notably in the women’s 50-m event. The block time was related for final performance in the men’s 50- m event with the old starting platform, but with the new platform it was critical only for the women’s 50-m event. Key Points The men had shorter block times than the women in both events and with both platforms. For both distances, the swimmers had shorter block times in their starts from the new starting platform with a back plate than with the old platform. For the 50-m event with the old starting platform, the medalists had shorter block times than the semi-finalists. The new starting platform block time was only determinant in the women’s 50-m event. In order to improve

  20. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Weissman, Jon B

    2006-04-30

    High performance computational science and engineering simulations have become an increasingly important part of the scientist's problem solving toolset. A key reason is the development of widely used codes and libraries that support these applications, for example, Netlib, a collection of numerical libraries [33]. The term community codes refers to those libraries or applications that have achieved some critical level of acceptance by a user community. Many of these applications are on the high-end in terms of required resources: computation, storage, and communication. Recently, there has been considerable interest in putting such applications on-line and packaging them as network services to make them available to a wider user base. Applications such as data mining [22], theorem proving and logic [14], parallel numerical computation [8][32] are example services that are all going on-line. Transforming applications into services has been made possible by advances in packaging and interface technologies including component systems [2][6][13][28][37], proposed communication standards [34], and newer Web technologies such as Web Services [38]. Network services allow the user to focus on their application and obtain remote service when needed by simply invoking the service across the network. The user can be assured that the most recent version of the code or service is always provided and they do not need to install, maintain, and manage significant infrastructure to access the service. For high performance applications in particular, the user is still often required to install a code base (e.g. MPI), and therefore become involved with the tedious details of infrastructure management. In the network service model, the service provider is responsible for all of these activities and not the user. The user need not become an expert in high performance computing. An additional advantage of high-end network services is that the user need not have specialized

  1. Newcastle disease virus and antibody levels in matched sera ...

    African Journals Online (AJOL)

    Haemagglutination inhibition assay was performed for all sera and egg yolk samples. Protective serum antibody titres of ≥3 (log2) were recorded in 5.3% of the naturally exposed, indigenous village hens. Antibody titers to Newcastle disease virus in the yolks were higher than in their sera (230.08 ± 40.05; 1.56 ± 0.74 for ...

  2. Periodontal status and Prevotella intermedia antibody in acute coronary syndrome.

    Science.gov (United States)

    Soejima, Hirofumi; Oe, Yoko; Nakayama, Hideki; Matsuo, Katsuhiko; Fukunaga, Takashi; Sugamura, Koichi; Kawano, Hiroaki; Sugiyama, Seigo; Shinohara, Masanori; Izumi, Yuichi; Ogawa, Hisao

    2009-11-12

    We performed periodontal examination and measured serum antibody levels against Prevotella intermedia in patients with acute coronary syndrome (ACS). Composite periodontal risk scores were significantly higher in the ACS group than in the coronary artery disease (CAD) group. Serum antibody levels were higher in the ACS group than in the CAD group and those were significantly correlated with the composite periodontal risk scores. These results provided important information about the status of P. intermedia infection in patients with ACS.

  3. Designing binding kinetic assay on the bio-layer interferometry (BLI) biosensor to characterize antibody-antigen interactions.

    Science.gov (United States)

    Kamat, Vishal; Rafique, Ashique

    2017-11-01

    The Octet biosensors provide a high-throughput alternative to the well-established surface plasmon resonance (SPR) and SPR imaging (SPRi) biosensors to characterize antibody-antigen interactions. However, the utility of the Octet biosensors for accurate and reproducible measurement of binding rate constants of monoclonal antibodies (mAbs) is limited due to challenges such as analyte rebinding, and mass transport limitation (MTL). This study focuses on addressing these challenges and provides experimental conditions to reliably measure kinetics of mAb-antigen interactions. The mAb capture density of less than 0.6 nm was found to be optimal to measure a wide range of binding affinities on Octet HTX biosensor. The titration kinetic and single cycle kinetic assays performed on Octet HTX generated reproducible binding kinetic parameters and correlated with the values measured on Biacore 4000 and MASS-1. Kinetic assays performed on 0.1 nm density mAb surfaces significantly reduced MTL and enabled characterization of picomolar affinity mAbs. Finally, kinetic analysis performed on 150 antibodies to 10 antigens with molecular weights ranging from 21kD to 105kD showed concordance between Octet HTX, Biacore 4000 and MASS-1 (R 2  > 0.90). The data presented in this study suggest that under optimal experimental conditions, Octet biosensor is capable of generating kinetic values comparable to SPR/SPRi biosensors. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  4. Asian American Literature: Resource and Training for Multi-Cultural Education. Final Reports: Program Performance and Financial Status. Ethnic Heritage Studies Program.

    Science.gov (United States)

    Ogawa, Dennis M.

    This is the report of a project designed to train secondary school teachers and other ethnic studies personnel in Hawaii in the area of Asian American literature so as to enhance their understanding of ethnic identity, interethnic relations, and the process of American multiculturalism. The project performance report includes a description of a…

  5. Identification performance of the IMS in the Middle East and North Africa. Final report, 10 August 1995--9 August 1997

    Energy Technology Data Exchange (ETDEWEB)

    Barker, T.G.; Baker, G.E.; McLaughlin, K.L.

    1998-01-01

    The authors have simulated the detection and identification performance of the proposed seismic network of the International Monitoring System (IMS) in the Middle East/North Africa. The identification performance of a network is strongly dependent upon regional source and propagation variability. However, knowledge of those variations permits estimation of their impact on the effectiveness of discriminants throughout the region. A comprehensive compilation of source and propagation properties was made and used to simulate network identification performance using the Monte Carlo program Xnice. The simulations described include a greater area (northwestern Africa) and use a far more extensive set of source and propagation parameters than the previous work (AD-A323 376). The authors find that the predicted performance of the Lg/P ratio and Lg slope discriminants ranges from extremely poor to extremely well within the study area, a result of the distribution of source propagation characteristics and station coverage. In another set of simulations, they have investigated the relationship between confidence ellipses estimated by the event location procedure and the distribution of locations found by the procedure. They find that the confidence ellipses are underestimates of the location variance.

  6. The effects of halides on the performance of coal gas-fueled molten carbonate fuel cells: Final report, October 1986-October 1987

    Energy Technology Data Exchange (ETDEWEB)

    Magee, T.P.; Kunz, H.R.; Krasij, M.; Cote, H.A.

    1987-10-01

    This report presents the results of a program to determine the probable tolerable limits of hydrogen chloride and hydrogen fluoride present in the fuel and oxidant streams of molten carbonate fuel cells that are operating on gasified coal. A literature survey and thermodynamic analyses were performed to determine the likely effects of halides on cell performance and materials. Based on the results of these studies, accelerated corrosion experiments and electrode half-cell performance tests were conducted using electrolyte which contained chloride and fluoride. These data and the results of previous in-cell tests were used to develop a computer for predicting the performance decay due to these halides. The tolerable limits were found to be low (less than 1 PPM) and depend on the power plant system configuration, the operating conditions of the fuel cell stack, the cell design and initial electrolyte inventory, and the ability of the cell to scrub low levels of halide from the reactant streams. The primary decay modes were conversion of the electrolyte from pure carbonate to a carbonate-halide mixture and accelerated electrolyte evaporation. 75 figs., 16 tabs.

  7. Hybrid FRP-concrete bridge deck system final report II : long term performance of hybrid FRP-concrete bridge deck system.

    Science.gov (United States)

    2009-06-01

    This report describes the investigation of the long term structural performance of a : hybrid FRP-concrete (HFRPC) bridge deck on steel girders. The study aimed at : assessing three long term aspects pertaining to the HFRPC bridge deck: (1) creep : c...

  8. Arsenic Removal from Drinking Water by Adsorptive Media U.S. EPA Demonstration Project at Spring Brook Mobile Home Park in Wales, ME Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed and the results obtained for the arsenic removal treatment technology demonstration project at Spring Brook Mobile Home Park (SBMHP) in Wales, Maine. The objectives of the project were to evaluate: 1) the effectiveness of an arsenic...

  9. Arsenic Removal from Drinking Water by Coagulation/Filtration - U.S. EPA Demonstration Project at Town of Arnaudville, LA - Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed during and the results obtained from the arsenic removal treatment technology demonstration project at the United Water Systems’ facility in Arnaudville, LA. The objectives of the project were to evaluate: (1) the effectiveness of K...

  10. Arsenic Removal from Drinking Water by Coagulation/Filtration - U.S. EPA Demonstration Project at Village of Waynesville, IL - Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed and the results obtained from the arsenic removal drinking water treatment technology demonstration project at the Village of Waynesville, IL. The main objective of the project was to evaluate the effectiveness of the Peerless coagu...

  11. Arsenic Removal from Drinking Water by Coagulation/Filtration, U.S. EPA Demonstration Project at the City of Okanogan, WA - Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed during and the results obtained from the arsenic removal treatment technology demonstration project at the City of Okanogan, WA facility. The objectives of the project were to evaluate: (1) the effectiveness of Filtronics’ FH-13 Ele...

  12. Arsenic Removal from Drinking Water by Iron Removal - U.S. EPA Demonstration Project at Northeastern Elementary School in Fountain City, IN - Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed and the results obtained from the arsenic removal treatment technology demonstration project at Northeastern Elementary School in Fountain City, IN. The main objective of the project was to evaluate the effectiveness of US Water Sys...

  13. ARSENIC REMOVAL FROM DRINKING WATER BY IRON REMOVAL. U.S. EPA DEMONSTRATION PROJECT AT CLIMAX, MN. FINAL PERFORMANCE EVALUATION REPORT.

    Science.gov (United States)

    This report documents the activities performed and the results obtained for the arsenic removal treatment technology demonstration project following one year of operation at the Climax, Minnesota, site. The objectives of the project were to evaluate: (1) the effectiveness of Kin...

  14. Arsenic Removal from Drinking Water by Iron Removal U.S. EPA Demonstration Project at Vintage on the Ponds in Delavan, WI Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed and the results obtained for the arsenic removal treatment technology demonstration project at Vintage on the Ponds in Delavan, WI. The objectives of the project were to evaluate: (1) the effectiveness of a Kinetico Macrolite® press...

  15. Arsenic Removal from Drinking Water by Iron Removal U.S. EPA Demonstration Project at Sabin, MN Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed and the results obtained from January 30, 2006 to April 29, 2007 at the U.S. Environmental Protection Agency (EPA) Arsenic Removal Technology Demonstration site in Sabin, MN. The main objective of the project was to evaluate the eff...

  16. Arsenic Removal from Drinking Water by Iron Removal and Adsorptive Media U.S. EPA Demonstration Project at Stewart, MN, Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed and the results obtained from the one-year U.S. Environmental Protection Agency (EPA) arsenic removal technology demonstration project at the Stewart, MN facility. The main objective of the project was to evaluate the effectiveness ...

  17. Arsenic Removal from Drinking Water by Adsorptive Media - U.S. EPA Demonstration Project at Woodstock Middle School in Woodstock, CT - Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed for and the results obtained from the arsenic removal treatment technology demonstration project at the Woodstock Middle School in Woodstock, CT. The objectives of the project were to evaluate the effectiveness of Adsorbsia™ GTO™ me...

  18. Arsenic Removal from Drinking Water by Adsorptive Media U.S. EPA Demonstration Project at Webb Consolidated Independent School District in Bruni, TX - Final Performance Evaluation Report

    Science.gov (United States)

    This report documents the activities performed and the results obtained from the arsenic removal treatment technology demonstration project at the Webb Consolidated Independent School District (Webb CISD) in Bruni, TX. The main objective of the project was to evaluate the effect...

  19. Development of New Low-Cost, High-Performance, PV Module Encapsulant/Packaging Materials: Final Technical Progress Report, 22 October 2002 - 15 November 2007

    Energy Technology Data Exchange (ETDEWEB)

    Tucker, R.

    2008-04-01

    Report on objectives to work with U.S.-based PV module manufacturers (c-Si, a-Si, CIS, other thin films) to develop/qualify new low-cost, high-performance PV module encapsulant/packaging materials, and processes using the packaging materials.

  20. Integrated Advanced Microwave Sounding Unit-A (AMSU-A). Performance Verification Reports: Final Comprehensive Performance Test Report, P/N: 1356006-1, S.N: 202/A2

    Science.gov (United States)

    Platt, R.

    1998-01-01

    This is the Performance Verification Report. the process specification establishes the requirements for the comprehensive performance test (CPT) and limited performance test (LPT) of the earth observing system advanced microwave sounding unit-A2 (EOS/AMSU-A2), referred to as the unit. The unit is defined on drawing 1356006.

  1. Intracellular antibody-mediated immunity and the role of TRIM21.

    Science.gov (United States)

    McEwan, William A; Mallery, Donna L; Rhodes, David A; Trowsdale, John; James, Leo C

    2011-11-01

    Protection against bacterial and viral pathogens by antibodies has always been thought to end at the cell surface. Once inside the cell, a pathogen was understood to be safe from humoral immunity. However, it has now been found that antibodies can routinely enter cells attached to viral particles and mediate an intracellular immune response. Antibody-coated virions are detected inside the cell by means of an intracellular antibody receptor, TRIM21, which directs their degradation by recruitment of the ubiquitin-proteasome system. In this article we assess how this discovery alters our view of the way in which antibodies neutralise viral infection. We also consider the antiviral function of TRIM21 in the context of its other reported roles in immune signalling and autoimmunity. Finally, we discuss the conceptual implications of intracellular antibody immunity and how it alters our view of the discrete separation of extracellular and intracellular environments. Copyright © 2011 WILEY Periodicals, Inc.

  2. A final report on the performance achieved by non-destructive testing of defective butt welds in 50mm thick Type 316 stainless steel

    International Nuclear Information System (INIS)

    Ford, J.; Hudgell, R.J.

    1987-03-01

    This report concludes a programme of work started approximately eight years ago to fabricate deliberately defective austenitic downhand welds in 50 mm thick Type 316 plate and then to examine them non-destructively under ideal laboratory conditions. After completing and reporting the Non-Destructive Testing (NDT), the specimens were subjected to detailed metallography to locate, identify and size all the planned and unplanned flaws in the welds. The report gives the final analysis of this exercise on the relative merits of X-radiography, pulse echo ultrasonics and the time-of-flight technique for the detection, location and sizing of weld flaws. It was found that X-radiography and pulse echo ultrasonics were the best techniques for flaw detection but neither technique was reliable for flaw sizing. The time-of-flight technique provided accurate sizing data but the location of the flaws had to be known to identify the diffracted signals from the extremities of the flaws due to the poor signal to noise ratio. Observations are also reported on the fabrication of deliberately defective austenitic welds for use as reference specimens in the FR programme. (author)

  3. The Goettingen high-Tc superconductivity research pool: the effects of structure and structural defects on the performance of high-Tc superconductors. Final reports

    International Nuclear Information System (INIS)

    1992-02-01

    The compilation presents the final reports prepared by the various teams of the Goettingen research pool for high-Tc superconductivity. The reports are entitled: Structure and phase transition in high-Tc superconductors (Krebs/Freyhardt). Preparation and critical properties of high-Tc superconductors (Freyhardt/Heinemann/Zimmermann). EMC measurements in high-Tc superconductors (Bormann/Noelting). Phase analysis of the various phases observed in the preparation of high-Tc superconductors (Faupel/Hehenkamp). Positron annihilation in high-Tc superconductors (Hehenkamp). Preparation and characterization of thin films consisting of superconducting oxide ceramics (v. Minnigerode/Samwer). High-Tc superconductivity in monocrystals (Winzer/Beuermann). Microwave conductivity in high-Tc superconductors (Helberg). High-resolution structural analyses in high-Tc superconductors (Kupcik/Bente). Synthesis, structural analyses and spectroscopy of high-Tc superconductors (Bente). Synthesis, monocrystal growing, crystal structure of high-Tc superconductors (Schwarzmann). Ion-beam-aided studies in high-Tc superconductors (Uhrmacher). (orig./MM) [de

  4. Effect of a clinical skills refresher course on the clinical performance, anxiety and self-efficacy of the final year undergraduate nursing students.

    Science.gov (United States)

    Shahsavari, Hooman; Ghiyasvandian, Shahrzad; Houser, Marian L; Zakerimoghadam, Masoumeh; Kermanshahi, Seyedeh Sosan Nouri; Torabi, Sedigheh

    2017-11-01

    Although the final year of nursing undergraduate programs that focus on clinical education are planned to prepare nursing students to better transition to the real world of health care service; evidence has shown that this program is not sufficient to reach this end goal. This controlled trial study was to investigate the effectiveness of a basic clinical skills refresher course for nursing students before entering the internship program. The sample consisted of 160 undergraduate nursing students assigned into two groups. The intervention was a three-day refresher course directed by nurse instructors for intervention group focused on 10 basic nursing procedures in the clinical skill lab. The control group did not receive any intervention. The students' anxiety, clinical self- efficacy and clinical skills practice were measured before and after intervention in both groups. The results indicated that the students who took part in the refresher course experienced lower anxiety levels, higher levels of clinical self-efficacy, and have better clinical skills during their internships. The undergraduate nursing curriculum can be strengthened by the basic clinical skills refresher course. This refresher course can bridge the theory - practice gap and provide a better transition from the student to nurse role. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Polyclonal antibodies for the detection of Trypanosoma cruzi circulating antigens.

    Directory of Open Access Journals (Sweden)

    Edith S Málaga-Machaca

    2017-11-01

    Full Text Available Detection of Trypanosoma cruzi antigens in clinical samples is considered an important diagnostic tool for Chagas disease. The production and use of polyclonal antibodies may contribute to an increase in the sensitivity of immunodiagnosis of Chagas disease.Polyclonal antibodies were raised in alpacas, rabbits, and hens immunized with trypomastigote excreted-secreted antigen, membrane proteins, trypomastigote lysate antigen and recombinant 1F8 to produce polyclonal antibodies. Western blot analysis was performed to determine specificity of the developed antibodies. An antigen capture ELISA of circulating antigens in serum, plasma and urine samples was developed using IgY polyclonal antibodies against T. cruzi membrane antigens (capture antibody and IgG from alpaca raised against TESA. A total of 33 serum, 23 plasma and 9 urine samples were analyzed using the developed test. Among serum samples, compared to serology, the antigen capture ELISA tested positive in 55% of samples. All plasma samples from serology positive subjects were positive in the antigen capture ELISA. All urine positive samples had corresponding plasma samples that were also positive when tested by the antigen capture ELISA.Polyclonal antibodies are useful for detection of circulating antigens in both the plasma and urine of infected individuals. Detection of antigens is direct evidence of the presence of the parasite, and could be a better surrogate of current infection status.

  6. The effects of tether placement on antibody stability on surfaces

    Science.gov (United States)

    Grawe, Rebecca W.; Knotts, Thomas A.

    2017-06-01

    Despite their potential benefits, antibody microarrays have fallen short of performing reliably and have not found widespread use outside of the research setting. Experimental techniques have been unable to determine what is occurring on the surface of an atomic level, so molecular simulation has emerged as the primary method of investigating protein/surface interactions. Simulations of small proteins have indicated that the stability of the protein is a function of the residue on the protein where a tether is placed. The purpose of this research is to see whether these findings also apply to antibodies, with their greater size and complexity. To determine this, 24 tethering locations were selected on the antibody Protein Data Bank (PDB) ID: 1IGT. Replica exchange simulations were run on two different surfaces, one hydrophobic and one hydrophilic, to determine the degree to which these tethering sites stabilize or destabilize the antibody. Results showed that antibodies tethered to hydrophobic surfaces were in general less stable than antibodies tethered to hydrophilic surfaces. Moreover, the stability of the antibody was a function of the tether location on hydrophobic surfaces but not hydrophilic surfaces.

  7. Performance of a diesel engine operating on raw coal-diesel fuel and solvent refined coal-diesel fuel slurries. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Marshall, H.P.

    1980-03-01

    Performance tests using an 11 kW single cylinder diesel engine were made to determine the effects of three different micronized coal-fuel oil slurries being considered as alternative fuels. Slurries containing 20, 32, and 40%-wt micronized raw coal in No. 2 fuel oil were used. Results are presented indicating the changes in the concentrations of SO/sub X/ and NO/sub X/ in the exhaust, exhaust opacity, power and efficiency, and in wear rates relative to operation on fuel oil No. 2. The engine was operated for 10 h at full load and 1400 rpm on al fuels except the 40%-wt slurry. This test was discontinued because of extremely poor performance.

  8. Performance Assessment of Bi-Directional Knotless Tissue-Closure Devices in Juvenile Chinook Salmon Surgically Implanted with Acoustic Transmitters, 2009 - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Woodley, Christa M.; Wagner, Katie A.; Bryson, Amanda J.

    2012-11-09

    The purpose of this report is to assess the performance of bi-directional knotless tissue-closure devices for use in tagging juvenile salmon. This study is part of an ongoing effort at Pacific Northwest National Laboratory (PNNL) to reduce unwanted effects of tags and tagging procedures on the survival and behavior of juvenile salmonids, by assessing and refining suturing techniques, suture materials, and tag burdens. The objective of this study was to compare the performance of the knotless (barbed) suture, using three different suture patterns (treatments: 6-point, Wide “N”, Wide “N” Knot), to the current method of suturing (MonocrylTM monofilament, discontinuous sutures with a 2×2×2×2 knot) used in monitoring and research programs with a novel antiseptic barrier on the wound (“Second Skin”).

  9. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    David W. Mazyck; Angela Lindner; CY Wu, Rick Sheahan, Ashok Jain

    2007-06-30

    Forest products provide essential resources for human civilization, including energy and materials. In processing forest products, however, unwanted byproducts, such as volatile organic compounds (VOCs) and hazardous air pollutants (HAPs) are generated. The goal of this study was to develop a cost effective and reliable air pollution control system to reduce VOC and HAP emissions from pulp, paper and paperboard mills and solid wood product facilities. Specifically, this work focused on the removal of VOCs and HAPs from high volume low concentration (HVLC) gases, particularly methanol since it is the largest HAP constituent in these gases. Three technologies were developed and tested at the bench-scale: (1) A novel composite material of activated carbon coated with a photocatalyst titanium dioxide (TiO{sub 2}) (referred to as TiO{sub 2}-coated activated carbon or TiO{sub 2}/AC), (2) a novel silica gel impregnated with nanosized TiO{sub 2} (referred to as silica-titania composites or STC), and (3) biofiltration. A pilot-scale reactor was also fabricated and tested for methanol removal using the TiO{sub 2}/AC and STC. The technical feasibility of removing methanol with TiO{sub 2}/AC was studied using a composite synthesized via a spay desiccation method. The removal of methanol consists of two consecutive operation steps: removal of methanol using fixed-bed activated carbon adsorption and regeneration of spent activated carbon using in-situ photocatalytic oxidation. Regeneration using photocatalytic oxidation employed irradiation of the TiO{sub 2} catalyst with low-energy ultraviolet (UV) light. Results of this technical feasibility study showed that photocatalytic oxidation can be used to regenerate a spent TiO{sub 2}/AC adsorbent. A TiO{sub 2}/AC adsorbent was then developed using a dry impregnation method, which performed better than the TiO{sub 2}/AC synthesized using the spray desiccation method. The enhanced performance was likely a result of the better

  10. Optimization of parameters affecting LSM performance as diffractors of x rays: Final technical report, 13 May 1986-31 October 1987

    International Nuclear Information System (INIS)

    Knight, L.V.

    1987-10-01

    Our efforts during this contract period were directed toward a deeper understanding of several important issues related to multilayer x-ray mirrors. The results have important practical applications. The topics studied included: Basic Stress-Strain-Curvature Theory for Thin Silicon Wafers with Oxide and Nitride Films; this theory and accompanying experimental verification could form the basis for development of a code that will be able to predict the final figure of a silicon substrate that has been etched and treated with oxide or nitride films; this code will allow the design and fabrication of sophisticated curved multilayers; we have applied our theoretical understanding of multilayers to the design of multilayer x-ray optics; Multilayer X-ray Data Analysis Codes; these codes allow the massaging and display of x-ray multilayer characterization data in a wide variety of graphical ways; the code is user friendly; simulation of multilayers behavior in a high x-ray flux environment; this code combines an x-ray input code (BUCKL) with a 1-D hydrodynamic code (Chart-D) and our own iterative multilayer design code to determine the reflectivity of multilayers as a function of time when the multilayer is in a region of high x-ray flux; BUCKL and Chart-D are from Sandia Labs.; and we have completed the first series of experimental measurements of multilayer reflectivity while subjected to a pulse of intense x-ray flux; the experiments were done with the Phoenix laser at LLNL; the results are included. 48 refs., 29 figs

  11. FINAL REPORT

    Energy Technology Data Exchange (ETDEWEB)

    Juergen Eckert; Anthony K. Cheetham (Principal Investigator)

    2011-03-11

    Hydrogen storage systems based on the readily reversible adsorption of H{sub 2} in porous materials have a number of very attractive properties with the potential to provide superior performance among candidate materials currently being investigated were it not for the fact that the interaction of H{sub 2} with the host material is too weak to permit viable operation at room temperature. Our study has delineated in quantitative detail the structural elements which we believe to be the essential ingredients for the future synthesis of porous materials, where guest-host interactions are intermediate between those found in the carbons and the metal hydrides, i.e. between physisorption and chemisorption, which will result in H{sub 2} binding energies required for room temperature operation. The ability to produce porous materials with much improved hydrogen binding energies depends critically on detailed molecular level analysis of hydrogen binding in such materials. However, characterization of H{sub 2} sorption is almost exclusively carried by thermodynamic measurements, which give average properties for all the sites occupied by H{sub 2} molecules at a particular loading. We have therefore extensively utilized the most powerful of the few molecular level experimental probes available to probe the interactions of hydrogen with porous materials, namely inelastic neutron scattering (INS) spectroscopy of the hindered rotations of the hydrogen molecules adsorbed at various sites, which in turn can be interpreted in a very direct way in by computational studies. This technique can relate spectral signatures of various H{sub 2} molecules adsorbed at binding sites with different degrees of interaction. In the course of this project we have synthesized a rather large number of entirely new hybrid materials, which include structural modifications for improved interactions with adsorbed hydrogen. The results of our systematic studies on many porous materials provide detailed

  12. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Gentile, Thomas R.

    2014-03-14

    We propose to extend the technique of polarized neutron scattering into new domains by continued development and application of polarized 3He spin-filters. These devices are particularly relevant to the Spallation Neutron Source, as the polarizing monochromators historically used at reactor sources will usually not be suitable polarizers, and wide-angle polarization analysis will be essential. With prior support from the Office of Science, we have developed neutron spin-filters based on the large spin dependence of the cross section for neutron capture by 3He, and applied these devices to a small angle neutron scattering spectrometer (SANS), polarized neutron reflectometers, a thermal energy single crystal diffractometer (SCD), and a thermal energy triple-axis instrument. Our developments have been adopted for application on the magnetism reflectometer at the SNS and for the NIST Center for Neutron Research (NCNR) 3He user capability. Results from both these programs are emerging. We have made significant progress in the past grant period on wide-angle polarization analysis. We have also performed several studies relevant to continuous optical pumping, including collaboration on experiments that have revealed neutron beam effects on spin filters that are continuously pumped by spin-exchange optical pumping. We contributed to an experiment on a neutron interferometer, in which the successful results obtained are directly related to both 3He cell technology and high accuracy 3He -based neutron polarimetry. Implementation of wide-angle polarization analysis will continue to be key thrust for our work in this new proposal. We will also focus on continuous optical pumping and the fundamental issues that determine the achievable 3He polarization. This project will be carried out by a collaboration involving scientists at NIST, Indiana University, Hamilton College, and the Univ. of Wisconsin who are experts in 3He polarization techniques, and materials scientists from the

  13. Final report on LDRD project : elucidating performance of proton-exchange-membrane fuel cells via computational modeling with experimental discovery and validation.

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Chao Yang (Pennsylvania State University, University Park, PA); Pasaogullari, Ugur (Pennsylvania State University, University Park, PA); Noble, David R.; Siegel, Nathan P.; Hickner, Michael A.; Chen, Ken Shuang

    2006-11-01

    In this report, we document the accomplishments in our Laboratory Directed Research and Development project in which we employed a technical approach of combining experiments with computational modeling and analyses to elucidate the performance of hydrogen-fed proton exchange membrane fuel cells (PEMFCs). In the first part of this report, we document our focused efforts on understanding water transport in and removal from a hydrogen-fed PEMFC. Using a transparent cell, we directly visualized the evolution and growth of liquid-water droplets at the gas diffusion layer (GDL)/gas flow channel (GFC) interface. We further carried out a detailed experimental study to observe, via direct visualization, the formation, growth, and instability of water droplets at the GDL/GFC interface using a specially-designed apparatus, which simulates the cathode operation of a PEMFC. We developed a simplified model, based on our experimental observation and data, for predicting the onset of water-droplet instability at the GDL/GFC interface. Using a state-of-the-art neutron imaging instrument available at NIST (National Institute of Standard and Technology), we probed liquid-water distribution inside an operating PEMFC under a variety of operating conditions and investigated effects of evaporation due to local heating by waste heat on water removal. Moreover, we developed computational models for analyzing the effects of micro-porous layer on net water transport across the membrane and GDL anisotropy on the temperature and water distributions in the cathode of a PEMFC. We further developed a two-phase model based on the multiphase mixture formulation for predicting the liquid saturation, pressure drop, and flow maldistribution across the PEMFC cathode channels. In the second part of this report, we document our efforts on modeling the electrochemical performance of PEMFCs. We developed a constitutive model for predicting proton conductivity in polymer electrolyte membranes and compared

  14. Change of the age and performance of swimmers across World Championships and Olympic Games finals from 1992 to 2013 - a cross-sectional data analysis.

    Science.gov (United States)

    König, Stefan; Valeri, Fabio; Wild, Stefanie; Rosemann, Thomas; Rüst, Christoph Alexander; Knechtle, Beat

    2014-01-01

    The aims of the present study were to investigate the changes in the age and in swimming performance of finalists in World Championships (1994-2013) and Olympic Games (1992-2012) competing in all events/races (stroke and distance). Data of 3,295 performances from 1,615 women and 1,680 men were analysed using correlation analyses and magnitudes of effect sizes. In the World Championships, the age of the finalists increased for all strokes and distances with exception of 200 m backstroke in women, and 400 m freestyle and 200 m breaststroke in men where the age of the finalists decreased. The magnitudes of the effects were small to very large (mean ± SD 2.8 ± 2.7), but extremely large (13.38) for 1,500 m freestyle in women. In the Olympic Games, the age of the finalists increased for all strokes and distances with exception of 800 m freestyle in women and 400 m individual medley in men. The magnitudes of the effects were small to very large (mean ± SD 4.1 ± 7.1), but extremely large for 50 m freestyle in women (10.5) and 200 m butterfly in men (38.0). Swimming performance increased across years in both women and men for all strokes and distances in both the World Championships and the Olympic Games. The magnitudes of the effects were all extremely large in World Championships (mean ± SD 20.1 ± 8.4) and Olympic Games (mean ± SD 52.1 ± 47.6); especially for 100 m and 200 m breaststroke (198) in women in the Olympic Games. To summarize, in the last ~20 years the age of the finalists increased in both the World Championships and the Olympic Games with some minor exceptions (200 m backstroke in women, 400 m freestyle and 200 m breaststroke in men in World Championships and 800 m freestyle in women and 400 m individual medley in men in Olympic Games) and performance of the finalists improved.

  15. Replacing antibodies with aptamers in lateral flow immunoassay.

    Science.gov (United States)

    Chen, Ailiang; Yang, Shuming

    2015-09-15

    Aptamers have been identified against various targets as a type of chemical or nucleic acid ligand by systematic evolution of ligands by exponential enrichment (SELEX) with high sensitivity and specificity. Aptamers show remarkable advantages over antibodies due to the nucleic acid nature and target-induced structure-switching properties and are widely used to design various fluorescent, electrochemical, or colorimetric biosensors. However, the practical applications of aptamer-based sensing and diagnostics are still lagging behind those of antibody-based tests. Lateral flow immunoassay (LFIA) represents a well established and appropriate technology among rapid assays because of its low cost and user-friendliness. The antibody-based platform is utilized to detect numerous targets, but it is always hampered by the antibody preparation time, antibody stability, and effect of modification on the antibody. Seeking alternatives to antibodies is an area of active research and is of tremendous importance. Aptamers are receiving increasing attention in lateral flow applications because of a number of important potential performance advantages. We speculate that aptamer-based LFIA may be one of the first platforms for commercial use of aptamer-based diagnosis. This review first gives an introduction to aptamer including the selection process SELEX with its focus on aptamer advantages over antibodies, and then depicts LFIA with its focus on aptamer opportunities in LFIA over antibodies. Furthermore, we summarize the recent advances in the development of aptamer-based lateral flow biosensing assays with the aim to provide a general guide for the design of aptamer-based lateral flow biosensing assays. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Evaluation of Gamma Interferon and Antibody Tuberculosis Tests in Alpacas

    Science.gov (United States)

    Holder, Tom; Clifford, Derek; Dexter, Ian; Brewer, Jacky; Smith, Noel; Waring, Laura; Crawshaw, Tim; Gillgan, Steve; Lyashchenko, Konstantin; Lawrence, John; Clarke, John; de la Rua-Domenech, Ricardo; Vordermeier, Martin

    2012-01-01

    We describe the performance of cell-based and antibody blood tests for the antemortem diagnosis of tuberculosis (TB) in South American camelids (SAC). The sensitivity and specificity of the gamma interferon (IFN-γ) release assay, two lateral flow rapid antibody tests (Stat-Pak and Dual Path Platform [DPP]), and two enzyme-linked immunosorbent assay (ELISA)-based antibody tests (Idexx and Enferplex) were determined using diseased alpacas from Mycobacterium bovis culture-confirmed breakdown herds and TB-free alpacas from geographical areas with no history of bovine TB, respectively. Our results show that while the sensitivities of the IFN-γ and antibody tests were similar (range of 57.7% to 66.7%), the specificity of the IFN-γ test (89.1%) was lower than those of any of the antibody tests (range of 96.4% to 97.4%). This lower specificity of the IFN-γ test was at least in part due to undisclosed Mycobacterium microti infection in the TB-free cohort, which stimulates a positive purified protein derivative (PPD) response. The sensitivity of infection detection could be increased by combining two antibody tests, but even the use of all four antibody tests failed to detect all diseased alpacas. These antibody-negative alpacas were IFN-γ positive. We found that the maximum sensitivity could be achieved only by the combination of the IFN-γ test with two antibody tests in a “test package,” although this resulted in decreased specificity. The data from this evaluation of tests with defined sensitivity and specificity provide potential options for antemortem screening of SAC for TB in herd breakdown situations and could also find application in movement testing and tracing investigations. PMID:22914362

  17. Pollen performance, cell number, and physiological state in the early-divergent angiosperm Annona cherimola Mill. (Annonaceae) are related to environmental conditions during the final stages of pollen development.

    Science.gov (United States)

    Lora, J; Herrero, M; Hormaza, J I

    2012-09-01

    Pollen performance is an important determinant for fertilization success, but high variability in pollen behavior both between and within species occurs in different years and under varying environmental conditions. Annona cherimola, an early-divergent angiosperm, is a species that releases a variable ratio of bicellular and tricellular hydrated pollen at anther dehiscence depending on temperature. The presence of both bi- and tricellular types of pollen is an uncommon characteristic in angiosperms and makes Annona cherimola an interesting model to study the effect of varying environmental conditions on subsequent pollen performance during the final stages of pollen development. In this work, we study the influence of changes in temperature and humidity during the final stages of pollen development on subsequent pollen performance, evaluating pollen germination, presence of carbohydrates, number of nuclei, and water content. At 25 °C, which is the average field temperature during the flowering period of this species, pollen had a viability of 60-70 %, starch hydrolyzed just prior to shedding, and pollen mitosis II was taking place, resulting in a mixture of bi- and tricellular pollen. This activity may be related to the pollen retaining 70 % water content at shedding. Temperatures above 30 °C resulted in a decrease in pollen germination, whereas lower temperatures did not have a clear influence on pollen germination, although they did have a clear effect on starch hydrolysis. On the other hand, slightly higher dehydration accelerated mitosis II, whereas strong dehydration arrested starch hydrolysis and reduced pollen germination. These results show a significant influence of environmental conditions on myriad pollen characteristics during the final stages of pollen development modifying subsequent pollen behavior and contributing to our understanding of the variability observed in pollen tube performance.

  18. Final Project Report: Composition and Realization of Source-to-Sink High-Performance Flows: File Systems, Storage, Hosts, LAN and WAN

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Chase [New Jersey Inst. of Technology, Newark, NJ (United States)

    2017-09-06

    A number of Department of Energy (DOE) science applications, involving exascale computing systems and large experimental facilities, are expected to generate large volumes of data, in the range of petabytes to exabytes, which will be transported over wide-area networks for the purpose of storage, visualization, and analysis. The objectives of this proposal are to (1) develop and test the component technologies and their synthesis methods to achieve source-to-sink high-performance flows, and (2) develop tools that provide these capabilities through simple interfaces to users and applications. In terms of the former, we propose to develop (1) optimization methods that align and transition multiple storage flows to multiple network flows on multicore, multibus hosts; and (2) edge and long-haul network path realization and maintenance using advanced provisioning methods including OSCARS and OpenFlow. We also propose synthesis methods that combine these individual technologies to compose high-performance flows using a collection of constituent storage-network flows, and realize them across the storage and local network connections as well as long-haul connections. We propose to develop automated user tools that profile the hosts, storage systems, and network connections; compose the source-to-sink complex flows; and set up and maintain the needed network connections.

  19. Powerplant Productivity Improvement Study: historic performance of Illinois investor-owned electrical-generating units. Final report, Project 2, Task 1

    Energy Technology Data Exchange (ETDEWEB)

    None

    1979-05-04

    In this task the historical performance of powerplants in Illinois was examined and the opportunities for improved powerplant productivity in Illinois was determined. The four utilities considered were the major investor-owned electric generating utilities in Illinois, i.e., Central Illinois Light Co., (CILCO), Central Illinois Public Service (CIPS), Commonwealth Edison (CECO), and Illinois Power (IP). The major findings are: (1) for evaluation purposes, the equivalent availability was judged to be the most-appropriate measure; (2) in terms of powerplant productivity, IP is among the best in the nation and, in terms of productivity from large coal units, it ranks in the top five nationally; (3) in general, the performance of coal-fired units of CILCO and CECO have been below national averages and in some cases, significantly below; (4) a review of the trends in production of all Illinois units reveals that Illinois units as a group have been generally below national averages for the respective unit classes; (5) as noted in this and other studies, productivities of the nuclear plants in Illinois have been below the national average for all comparable nuclear plants; and (6) this analysis should be updated when national 1977 and 1978 Edison Electric Institute data become publicly available.

  20. Dissecting Immunogenicity of Monoclonal Antibodies

    National Research Council Canada - National Science Library

    Snyder, Christopher

    2002-01-01

    The potential of monoclonal antibodies, (mAbs), for use in therapeutic and diagnostic applications has not been fully realized in part due to counter-immune responses that often arise in patient recipients of mAb...

  1. Dissecting Immunogenicity of Monoclonal Antibodies

    National Research Council Canada - National Science Library

    Snyder, Christopher

    2003-01-01

    The potential of monoclonal antibodies, (mAbs), for use in therapeutic and diagnostic applications has not been fully realized in part due to counter-immune responses that often arise in patient recipients of mAb...

  2. Antisperm antibodies and fertility association.

    Science.gov (United States)

    Restrepo, B; Cardona-Maya, W

    2013-10-01

    To evaluate the relation between antisperm antibodies (ASA) and human fertility by reviewing the scientific literature of the last 45 years. We carried out a review of scientific literature about antisperm antibodies and infertility published in spanish or english in databases as Pubmed, Medline, Scielo, some books and another gray literature include information related to this review and that is published in the last 45 years. Infertile couples suffer infertility by immunological mechanisms mainly by the presence of antisperm antibodies ASA in blood, semen or cervicovaginal secretions; the formation of ASA in men and women may be associated with disturbance in immunomodulatory mechanisms that result in functional impairment of sperm and thus its inability to fertilize the oocyte. Immunological infertility caused by ASA is the result of interference of these antibodies in various stages of fertilization process, inhibiting the ability of interaction between sperm and oocyte. Copyright © 2012 AEU. Published by Elsevier Espana. All rights reserved.

  3. Antibody Drug Conjugates: Preclinical Considerations.

    Science.gov (United States)

    Bornstein, Gadi G

    2015-05-01

    The development path for antibody drug conjugates (ADCs) is more complex and challenging than for unmodified antibodies. While many of the preclinical considerations for both unmodified and antibody drug conjugates are shared, special considerations must be taken into account when developing an ADC. Unlike unmodified antibodies, an ADC must preferentially bind to tumor cells, internalize, and traffic to the appropriate intracellular compartment to release the payload. Parameters that can impact the pharmacological properties of this class of therapeutics include the selection of the payload, the type of linker, and the methodology for payload drug conjugation. Despite a plethora of in vitro assays and in vivo models to screen and evaluate ADCs, the challenge remains to develop improved preclinical tools that will be more predictive of clinical outcome. This review will focus on preclinical considerations for clinically validated small molecule ADCs. In addition, the lessons learned from Mylotarg®, the first in class FDA-approved ADC, are highlighted.

  4. KrF laser fusion: perform optical systems study of direct short pulse extraction. Final technical report, 26 June 1978-14 March 1981

    International Nuclear Information System (INIS)

    Bradford, R.S. Jr.; Betts, J.A.; Aprahamian, R.; Livingston, P.M.

    1981-03-01

    KrF is a prime candidate for a laser inertial confinement fusion (ICF) driver, however, there are still scaling issues to be addressed. The purpose of this program is to determine KrF performance in a regime of ICF interest both experimentally and analytically. Using a master oscillator-power amplifier (MOPA) configuration, 100J has been obtained from a five liter optical volume KrF amplifier pumped at 300 KW/cm 3 peak at the end of a 500 nsec pulse. A normalized input flux of 0.4 was required to reduce amplified spontaneous emission (ASE) to one half the free running value. A peak extraction efficiency of 0.46 was obtained under these conditions at 1 atm total pressure. The TRW kinetics model has been refined to give excellent agreement with high and low pump regimes. An integrated kinetics and pulse propagation code has also been developed and agrees well with experiment

  5. Performance and evaluation of gas-engine-driven rooftop air conditioning equipment at the Willow Grove Naval Air Station. Final report (revised October 21, 1996)

    Energy Technology Data Exchange (ETDEWEB)

    Armstrong, P.R.; Katipamula, S.

    1996-10-01

    The performance was evaluated of a new US cooling technology that has been installed for the first time at a federal facility. The technology is a 15-ton natural gas-engine-driven rooftop air conditioning unit made by Thermo King. Two units were installed to serve the Navy Exchange at Willow Grove. The savings potential at Willow Grove is described and that in the federal sector estimated. Conditions for implementation are discussed. In summary, the new technology is generally cost-effective at sites where marginal electricity cost (per MBtu at the meter) is more than 4 times the marginal gas cost (per MBtu at the meter) and annual full-load-equivalent cooling hours exceed 2,000.

  6. Measurement of charge asymmetry of top quark-antiquark pairs in di-leptonic final states performed at D0 and ATLAS detectors

    International Nuclear Information System (INIS)

    Chapelain, Antoine

    2014-01-01

    Particle physics aims to give a coherent description of the nature and the behavior of elementary particles of matter. Particle accelerators (colliders) allow pushing back our knowledge in this domain producing particles that cannot be observed by other means. This thesis work contributes to this research field and focuses on the study of the top quark which is the latest brick of matter discovered and the heaviest known elementary particle. The property of the top quark studied here, the charge asymmetry of the top quark-antiquark pairs, has driven a lot of attention in 2011 because of measurements released by Tevatron experiments. These measurements showed deviations with the predictions made in the framework of the standard model of particle physics. New measurements of the charge asymmetry performed at the Tevatron (with the D0 detector) and at the LHC (with the ATLAS detector) are presented in this thesis. (author) [fr

  7. Study of nonstandard auto-antibodies as prognostic markers in auto immune hepatitis in children

    Directory of Open Access Journals (Sweden)

    Mahmoud Nermine H

    2009-07-01

    Full Text Available Abstract Background Antibodies to chromatin and soluble liver antigen have been associated with severe form of autoimmune hepatitis and/or poor treatment response and may provide guidance in defining subsets of patients with different disease behaviors. The major clinical limitation of these antibodies is their lower individual occurrence in patients with autoimmune hepatitis. Aim To estimate the value of detection of these non-standard antibodies in autoimmune hepatitis as prognostic markers. Methods Both antibodies were tested by enzyme immunoassay in 20 patients with autoimmune hepatitis. Results Antibodies to soluble liver antigen were not detected in any of our patients. On the other hand anti chromatin antibodies were present in 50% (10/20. Antibodies to chromatin occurred more commonly in females than males (8/14 versus 2/6. Of the 14 patients who relapsed 8(57% had antichromatin antibodies while they were present in only 2 out of 6(33.3% non relapsers. Antichromatin antibodies were found more in patients with antinuclear (3/4 and anti smooth muscle antibodies (9/13 more than in those with liver kidney microsomal antibodies (1/4 and those seronegative (1/4 i.e. they were +ve in patients with type I (8/12(66.6% more than those with type II (1/4(25% and those seronegative (1/4(25%. Antibodies to chromatin are associated with high levels of γ globulin but yet with no statistical difference between seropositive and seronegative counterparts (p = 0.65. Conclusion Antibodies to chromatin may be superior than those to soluble liver antigen in predicting relapse and may be useful as prognostic marker. Further studies with larger number of patients and combined testing of more than one antibody will improve the performance parameters of these antibodies and define optimal testing conditions for them before they can be incorporated into management algorithms that project prognosis.

  8. Development of a high-performance coal-fired power generating system with pyrolysis gas and char-fired high temperature furnace (HITAF). Volume 1, Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-02-01

    A major objective of the coal-fired high performance power systems (HIPPS) program is to achieve significant increases in the thermodynamic efficiency of coal use for electric power generation. Through increased efficiency, all airborne emissions can be decreased, including emissions of carbon dioxide. High Performance power systems as defined for this program are coal-fired, high efficiency systems where the combustion products from coal do not contact the gas turbine. Typically, this type of a system will involve some indirect heating of gas turbine inlet air and then topping combustion with a cleaner fuel. The topping combustion fuel can be natural gas or another relatively clean fuel. Fuel gas derived from coal is an acceptable fuel for the topping combustion. The ultimate goal for HIPPS is to, have a system that has 95 percent of its heat input from coal. Interim systems that have at least 65 percent heat input from coal are acceptable, but these systems are required to have a clear development path to a system that is 95 percent coal-fired. A three phase program has been planned for the development of HIPPS. Phase 1, reported herein, includes the development of a conceptual design for a commercial plant. Technical and economic feasibility have been analysed for this plant. Preliminary R&D on some aspects of the system were also done in Phase 1, and a Research, Development and Test plan was developed for Phase 2. Work in Phase 2 include s the testing and analysis that is required to develop the technology base for a prototype plant. This work includes pilot plant testing at a scale of around 50 MMBtu/hr heat input. The culmination of the Phase 2 effort will be a site-specific design and test plan for a prototype plant. Phase 3 is the construction and testing of this plant.

  9. Benchmark Analyses on the Natural Circulation Test Performed During the PHENIX End-of-Life Experiments. Final Report of a Co-ordinated Research Project 2008-2011

    International Nuclear Information System (INIS)

    2013-07-01

    The International Atomic Energy Agency (IAEA) supports Member State activities in the area of advanced fast reactor technology development by providing a forum for information exchange and collaborative research programmes. The Agency's activities in this field are mainly carried out within the framework of the Technical Working Group on Fast Reactors (TWG-FR), which assists in the implementation of corresponding IAEA activities and ensures that all technical activities are in line with the expressed needs of Member States. Among its broad range of activities, the IAEA proposes and establishes coordinated research projects (CRPs) aimed at the improvement of Member State capabilities in the area of fast reactor design and analysis. An important opportunity to undertake collaborative research was provided by the experimental campaign of the French Alternative Energies and Atomic Energy Commission (CEA) in the prototype sodium fast reactor PHENIX before it was shut down in 2009. The overall purpose of the end of life tests was to gather additional experience on the operation of sodium cooled reactors. As the CEA opened the experiments to international cooperation, in 2007 the IAEA launched a CRP on ''Control Rod Withdrawal and Sodium Natural Circulation Tests Performed during the PHENIX End-of-Life Experiments''. The CRP, with the participation of institutes from eight countries, contributed to improving capabilities in sodium cooled reactor simulation through code verification and validation, with particular emphasis on temperature and power distribution calculations and the analysis of sodium natural circulation phenomena. The objective of this report is to document the results and main achievements of the benchmark analyses on the natural circulation test performed in the framework of the PHENIX end of life experimental campaign

  10. Final report on accident tolerant fuel performance analysis of APMT-Steel Clad/UO₂ fuel and APMT-Steel Clad/UN-U₃Si₅ fuel concepts

    Energy Technology Data Exchange (ETDEWEB)

    Unal, Cetin [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Galloway, Jack D. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2014-09-12

    In FY2014 our group completed and documented analysis of new Accident Tolerant Fuel (ATF) concepts using BISON. We have modeled the viability of moving from Zircaloy to stainless steel cladding in traditional light water reactors (LWRs). We have explored the reactivity penalty of this change using the MCNP-based burnup code Monteburns, while attempting to minimize this penalty by increasing the fuel pellet radius and decreasing the cladding thickness. Fuel performance simulations using BISON have also been performed to quantify changes to structural integrity resulting from thinner stainless steel claddings. We account for thermal and irradiation creep, fission gas swelling, thermal swelling and fuel relocation in the models for both Zircaloy and stainless steel claddings. Additional models that account for the lower oxidation stainless steel APMT are also invoked where available. Irradiation data for HT9 is used as a fallback in the absence of appropriate models. In this study the isotopic vectors within each natural element are varied to assess potential reactivity gains if advanced enrichment capabilities were levied towards cladding technologies. Recommendations on cladding thicknesses for a robust cladding as well as the constitutive components of a less penalizing composition are provided. In the first section (section 1-3), we present results accepted for publication in the 2014 TOPFUEL conference regarding the APMT/UO₂ ATF concept (J. Galloway & C. Unal, Accident Tolerant and Neutronically Favorable LWR Cladding, Proceedings of WRFPM 2014, Sendai, Japan, Paper No.1000050). Next we discuss our preliminary findings from the thermo-mechanical analysis of UN-U₃Si₅ fuel with APMT clad. In this analysis we used models developed from limited data that need to be updated when the irradiation data from ATF-1 test is available. Initial results indicate a swelling rate less than 1.5% is needed to prevent excessive clad stress.

  11. Performance and completion assessment of an in-situ aerated municipal solid waste landfill - Final scientific documentation of an Austrian case study.

    Science.gov (United States)

    Hrad, Marlies; Huber-Humer, Marion

    2017-05-01

    By converting anaerobic landfills into a biologically stabilized state through accelerating aerobic organic matter degradation, the effort and duration necessary for post-closure procedures can be shortened. In Austria, the first full-scale application of in-situ landfill aeration by means of low pressure air injection with simultaneous off-gas collection and treatment was implemented on an old MSW-landfill and operated between 2007 and 2013. Besides complementary laboratory investigations, which included waste sampling from the landfill site prior to aeration start, a comprehensive field monitoring program was conducted to assess the influence of the aeration measure on the emission behavior of the landfilled waste during the aeration period as well as after aeration completion. Although the initial waste material was described as rather stable, the lab-scale aeration tests indicated a significant improvement of the leachate quality and even the biological solid waste stability. However, the aeration success was less pronounced for the application at the landfill site, mainly due to technical limitations in the full-scale operation. In this paper main performance data of the field investigation are compared to four other scientifically documented case studies along with stability indicators for solid waste and leachate characteristics in order to evaluate the success of aeration as well as the progress of a landfill towards completion and end of post-closure care. A number of quantitative benchmarks and relevant context information for the performance assessment of the five hitherto conducted international aeration projects are proposed aiming to support the systematization and harmonization of available results from diverse field studies and full-scale applications in future. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. High-throughput immunoturbidimetric assays for in-process determination of polyclonal antibody concentration and functionality in crude samples

    DEFF Research Database (Denmark)

    Bak, Hanne; Kyhse-Andersen, J.; Thomas, O.R.T.

    2007-01-01

    We present fast, simple immunoturbidimetric assays suitable for direct determination of antibody 'concentration' and 'functionality' in crude samples, such as in-process samples taken at various stages during antibody purification. Both assays display excellent linearity and analytical recovery. ...... antibodies, require only basic laboratory equipment, are robust, fast, cheap, easy to perform, and readily adapted to automation....

  13. Development of radiolabelling techniques of anti-CEA monoclonal antibody

    International Nuclear Information System (INIS)

    Castiglia, S.G. de

    1998-01-01

    The purpose of this work was to label monoclonal and polyclonal antibodies with 99 Tc m such as the ior-CEA-1 antibody and polyclonal IgG using a direct method, to check the radiochemical and biological behavior of labelled products, to prepare it under sterile and apyrogenic conditions as a lyophilized kit and to employ it in clinical trials. In addition, a photoactivation method was used to label polyclonal IgG with 99 Tc m and to compare with the established method using mercaptoethanol (2-ME) as the reducing agent. Finally polyclonal IgG was labelled using an indirect method in which a chelator was covalently attached to the protein and the 99 Tc m added as glucoheptonate complex. The properties of 99 Tc m when labelled with monoclonal and polyclonal antibodies by different methods were assessed by in vitro and in vivo studies

  14. Radiolabeled monoclonal antibodies: a review

    International Nuclear Information System (INIS)

    Toledo e Souza, I.T. de; Okada, H.

    1990-05-01

    Since the description by Kohler and Milstein 1975 of their technique for producing monoclonal antibodies of predefined specificity, it has become a mainstay in most laboratories that utilize immunochemical techniques to study problems in basic, applied or clinical research. Paradoxically, the very success of monoclonal antibodies has generated a literature which is now so vast and scattered that it has become difficult to obtain a perspective. This brief review represents the distillation of many publications relating to the production and use of monoclonaal antibodies as radiopharmaceuticals. Significant advances were made possible in the last few years by combined developments in the fields of tumor-associated antigens and of monoclonal antibodies. In fact monoclonal antibodies against some well defined tumor-associated antigens, has led to significantly greater practical possibilities for producing highly specific radiolabeled antibodies as radiopharmaceuticals for diagnosis and therapy of human tumors. One of the main requirements of this methodology is the availability of stable radiopharmaceutical reagents which after labeling in vivo injection retain the capacity of specific interaction with the defined antigen and their molecular integrity. Since injection into human is the objetive of this kind of study all the specifications of radiopharmaceutical have to be fulfilled e.g. sterility, apirogenicity and absence of toxicity. (author) [pt

  15. Avian Diagnostic and Therapeutic Antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Bradley, David Sherman [UND SMHS

    2012-12-31

    A number of infectious agents have the potential of causing significant clinical symptomology and even death, but dispite this, the number of incidence remain below the level that supports producing a vaccine. Therapeutic antibodies provide a viable treatment option for many of these diseases. We proposed that antibodies derived from West Nile Virus (WNV) immunized geese would be able to treat WNV infection in mammals and potential humans. We demonstrated that WNV specific goose antibodies are indeed successful in treating WNV infection both prophylactically and therapeutically in a golden hamster model. We demonstrated that the goose derived antibodies are non-reactogenic, i.e. do not cause an inflammatory response with multiple exposures in mammals. We also developed both a specific pathogen free facility to house the geese during the antibody production phase and a patent-pending purification process to purify the antibodies to greater than 99% purity. Therefore, the success of these study will allow a cost effective rapidly producible therapeutic toward clinical testing with the necessary infrastructure and processes developed and in place.

  16. Tabhu: tools for antibody humanization.

    KAUST Repository

    Olimpieri, Pier Paolo

    2014-10-09

    SUMMARY: Antibodies are rapidly becoming essential tools in the clinical practice, given their ability to recognize their cognate antigens with high specificity and affinity, and a high yield at reasonable costs in model animals. Unfortunately, when administered to human patients, xenogeneic antibodies can elicit unwanted and dangerous immunogenic responses. Antibody humanization methods are designed to produce molecules with a better safety profile still maintaining their ability to bind the antigen. This can be accomplished by grafting the non-human regions determining the antigen specificity into a suitable human template. Unfortunately, this procedure may results in a partial or complete loss of affinity of the grafted molecule that can be restored by back-mutating some of the residues of human origin to the corresponding murine ones. This trial-and-error procedure is hard and involves expensive and time-consuming experiments. Here we present tools for antibody humanization (Tabhu) a web server for antibody humanization. Tabhu includes tools for human template selection, grafting, back-mutation evaluation, antibody modelling and structural analysis, helping the user in all the critical steps of the humanization experiment protocol. AVAILABILITY: http://www.biocomputing.it/tabhu CONTACT: anna.tramontano@uniroma1.it, pierpaolo.olimpieri@uniroma1.it SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

  17. Monoclonal antibodies to carcino-embryonic antigen

    International Nuclear Information System (INIS)

    Teh, Jinghee; McKenzie, I.F.C.

    1990-01-01

    With the aim of producing new MoAb to colorectal carcinoma, immunization with cell suspensions of a fresh colonic tumour was performed and MoAb 17C4 was obtained. To produce other MoAb to colon cancer, an immunization protocol using fresh tumour, colonic cell lines and sera from patients with colonic tumours was employed and resulted in MoAb JGT-13, LK-4 and XPX-13. MoAb I-1 and O-1 were raised against sera from patients with colon cancer to produce MoAb directed against circulating tumour associated antigens. The six antibodies gave a range of reactions with normal and malignant tissues, indicating that they most likely reacted with different epitopes. Thus, apart from the reactions of 17C4, LK-4 and XPX-13 with fresh and formalin-fixed granulocytes, none of the antibodies reacted with formalin-fixed normal tissues. Despite the apparent specificity of these MoAb for colon cancer, serum testing using MoAb gave similar results to carcino-embryonic antigen polyclonal antibodies, that is the MoAb gave no obvious advantage. 9 refs., 1 tab., 3 figs

  18. Antibody purification using affinity chromatography: a case study with a monoclonal antibody to ractopamine.

    Science.gov (United States)

    Wang, Zhanhui; Liang, Qi; Wen, Kai; Zhang, Suxia; Shen, Jianzhong

    2014-11-15

    The application of antibodies to small molecules in the field of bioanalytics requires antibodies with stable biological activity and high purity; thus, there is a growing interest in developing rapid, inexpensive and effective procedures to obtain such antibodies. In this work, a ractopamine (RAC) derivative, N-4-aminobutyl ractopamine (ABR), was synthesized for preparing new specific affinity chromatography to purify a murine monoclonal antibody (mAb) against RAC from ascites. The performance of the new specific chromatography was compared with four other purification methods in terms of recovery, purity and biological activity of mAb. These four purification methods were prepared by using specific ligands (RAC and RAC-ovalbumin) and commercial ligands (protein G and protein A), respectively. The results showed that the highest recovery (88.1%) was achieved using the new chromatography; in comparison, the recoveries from the other methods were all below 70%. The purity of the mAbs from the new chromatography was 88.3%, while, the highest purity of 97.6% was from protein G chromatography and the lowest purity of 84.7% was from protein A chromatography. The biological activity of the purified mAb from all of the chromatography methods was comparable in enzyme-linked immunosorbent immunoassay (ELISA). Copyright © 2014 Elsevier B.V. All rights reserved.

  19. High Efficiency Water Heating Technology Development Final Report. Part I, Lab/Field Performance Evaluation and Accelerated Life Testing of a Hybrid Electric Heat Pump Water Heater (HPWH)

    Energy Technology Data Exchange (ETDEWEB)

    Baxter, Van D. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Murphy, Richard W. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Rice, C. Keith [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Linkous, Randall Lee [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2016-04-01

    DOE has supported efforts for many years with the objective of getting a water heater that uses heat pump technology (aka a heat pump water heater or HPWH) successfully on the residential equipment market. The most recent previous effort (1999-2002) produced a product that performed very well in ORNL-led accelerated durability and field tests. The commercial partner for this effort, Enviromaster International (EMI), introduced the product to the market under the trade name Watter$aver in 2002 but ceased production in 2005 due to low sales. A combination of high sales price and lack of any significant infrastructure for service after the sale were the principal reasons for the failure of this effort. What was needed for market success was a commercial partner with the manufacturing and market distribution capability necessary to allow economies of scale to lead to a viable unit price together with a strong customer service infrastructure. General Electric certainly meets these requirements, and knowing of ORNL s expertise in this area, approached ORNL with the proposal to partner in a CRADA to produce a high efficiency electric water heater. A CRADA with GE was initiated early in Fiscal Year, 2008. GE initially named its product the Hybrid Electric Water Heater (HEWH).

  20. A Microfabricated Segmented-Involute-Foil Regenerator for Enhancing Reliability and Performance of Stirling Engines. Phase III Final Report for the Radioisotope Power Conversion Technology NRA

    Science.gov (United States)

    Ibrahim, Mounir B.; Gedeon, David; Wood, Gary; McLean, Jeffrey

    2009-01-01

    Under Phase III of NASA Research Announcement contract NAS3-03124, a prototype nickel segmented-involute-foil regenerator was microfabricated and tested in a Sunpower Frequency-Test-Bed (FTB) Stirling convertor. The team for this effort consisted of Cleveland State University, Gedeon Associates, Sunpower Inc. and International Mezzo Technologies. Testing in the FTB convertor produced about the same efficiency as testing with the original random-fiber regenerator. But the high thermal conductivity of the prototype nickel regenerator was responsible for a significant performance degradation. An efficiency improvement (by a 1.04 factor, according to computer predictions) could have been achieved if the regenerator was made from a low-conductivity material. Also, the FTB convertor was not reoptimized to take full advantage of the microfabricated regenerator s low flow resistance; thus, the efficiency would likely have been even higher had the FTB been completely reoptimized. This report discusses the regenerator microfabrication process, testing of the regenerator in the Stirling FTB convertor, and the supporting analysis. Results of the pre-test computational fluid dynamics (CFD) modeling of the effects of the regenerator-test-configuration diffusers (located at each end of the regenerator) are included. The report also includes recommendations for further development of involute-foil regenerators from a higher-temperature material than nickel.

  1. Use of isotopic tracers in studies of herbicides performance on grasses and sedges. Report of a final research co-ordination meeting

    International Nuclear Information System (INIS)

    1998-02-01

    Herbicide products as sold to the user are a mixture or formulation of active ingredient, surfactants and other adjuvants, plus a carrier, which will be a liquid if the formulation is to be applied as a spray. The adjuvants affect the characteristics of the spray, including the retention of the droplets by plant surfaces and the penetration of active ingredient into the plant. Thus they play a critical part in determining the phototoxicity and selectivity of the product. The IAEA organized a co-ordinated research programme in 1992 to explore the possibility of improving the performance of the herbicide glyphosate on Cyperus rotundus (purple nutsedge), commonly regarded as the ''world's worst weed'', by modifying the commercial formulation using penetration studies with 14 C labelled glyphosate as the initial screening procedure. This TECDOC summarizes the outcome of the programme and includes the papers presented at the research co-ordination meeting held in Los Banos, Philippines, 17-21 February 1997. The co-operation of the Monsanto Company, the manufacturer of the glyphosate herbicide, is gratefully acknowledged

  2. Engineering development of coal-fired high performance power systems, Phases 2 and 3. Quarterly progress report, October 1--December 31, 1996. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-31

    The goals of this program are to develop a coal-fired high performance power generation system (HIPPS) by the year 2000 that is capable of: {gt} 47% efficiency (HHV); NO{sub x}, SO{sub x}, and particulates {gt} 10% NSPS; coal providing {ge} 65% of heat input; all sold wastes benign; and cost of electricity 90% of present plant. Work reported herein is from Task 1.3 HIPPS Commercial Plant Design, Task 2,2 HITAF Air Heater, and Task 2.4 Duct Heater Design. The impact on cycle efficiency from the integration of various technology advances is presented. The criteria associated with a commercial HIPPS plant design as well as possible environmental control options are presented. The design of the HITAF air heaters, both radiative and convective, is the most critical task in the program. In this report, a summary of the effort associated with the radiative air heater designs that have been considered is provided. The primary testing of the air heater design will be carried out in the UND/EERC pilot-scale furnace; progress to date on the design and construction of the furnace is a major part of this report. The results of laboratory and bench scale activities associated with defining slag properties are presented. Correct material selection is critical for the success of the concept; the materials, both ceramic and metallic, being considered for radiant air heater are presented. The activities associated with the duct heater are also presented.

  3. Final report for %22High performance computing for advanced national electric power grid modeling and integration of solar generation resources%22, LDRD Project No. 149016.

    Energy Technology Data Exchange (ETDEWEB)

    Reno, Matthew J.; Riehm, Andrew Charles; Hoekstra, Robert John; Munoz-Ramirez, Karina; Stamp, Jason Edwin; Phillips, Laurence R.; Adams, Brian M.; Russo, Thomas V.; Oldfield, Ron A.; McLendon, William Clarence, III; Nelson, Jeffrey Scott; Hansen, Clifford W.; Richardson, Bryan T.; Stein, Joshua S.; Schoenwald, David Alan; Wolfenbarger, Paul R.

    2011-02-01

    Design and operation of the electric power grid (EPG) relies heavily on computational models. High-fidelity, full-order models are used to study transient phenomena on only a small part of the network. Reduced-order dynamic and power flow models are used when analysis involving thousands of nodes are required due to the computational demands when simulating large numbers of nodes. The level of complexity of the future EPG will dramatically increase due to large-scale deployment of variable renewable generation, active load and distributed generation resources, adaptive protection and control systems, and price-responsive demand. High-fidelity modeling of this future grid will require significant advances in coupled, multi-scale tools and their use on high performance computing (HPC) platforms. This LDRD report demonstrates SNL's capability to apply HPC resources to these 3 tasks: (1) High-fidelity, large-scale modeling of power system dynamics; (2) Statistical assessment of grid security via Monte-Carlo simulations of cyber attacks; and (3) Development of models to predict variability of solar resources at locations where little or no ground-based measurements are available.

  4. A sensitive progesterone enzyme immunoassay for cow, goat and llama plasma using a monoclonal antibody and Danazol (17-α-2,4-pregnadien-20-yno (2,3-D) isoxazol-17-ol) as a displacing agent

    International Nuclear Information System (INIS)

    Aba, M.A.; Carlsson, M.A.; Karlsson, A.; Forsberg, M.

    2001-01-01

    A sensitive progesterone enzyme immunoassay was developed for cow, goat and llama plasma using a monoclonal antibody and Danazol (17-α-2,4-pregnadien-20-yno (2,3-d) isoxazol-17-ol) as a displacing agent. The microtitration plates were first coated with progesterone 3 (o-carboxy-methyl) oxine: BSA conjugate. The immune reaction was performed by incubating overnight a mixture of 50 μL of plasma and 100 μL of first antibody. After washing, 100 μL of the second antibody (horse radish peroxidase conjugated anti-mouse IgG) were added. The plates were incubated for 1 hour and washed. Immediately the substrate solution was added and finally the reaction stopped and optical density measured. This assay allows accurate determination of progesterone in plasma from several species with good specificity, precision and accuracy, and is suitable for the rapid assessment of luteal function and reproductive status in both clinical and research situations. (author)

  5. The study of conjugation of anti-CD20 monoclonal antibody for labeling with metallic or lanthanides radionuclides

    International Nuclear Information System (INIS)

    Akanji, Akinkunmi Ganiyu

    2012-01-01

    Lymphomas are malignancies or cancers that start from the malign transformation of a lymphocyte in the lymphatic system. Generally, lymphomas start from the lymph nodes or from the agglomeration of the lymphatic tissues, organs like stomach, intestines, in some cases it can involve the bone marrow and the blood, it can also disseminate to other organs. Lymphomas are divided in two major categories: Hodgkin lymphoma and non-Hodgkin lymphoma (NHL). Patient with NHL are generally treated with radiotherapy alone or combined with immunotherapy using monoclonal antibody rituximab (MabThera®). Currently, monoclonal antibodies (Acm) conjugated with bifunctional chelate agents and radiolabeled with metallic or lanthanides radionuclides are a treatment reality for patients with NHL by the principle of radioimmunotherapy (RIT). This study focused on the conditions of conjugation of Acm rituximab (MabThera®) with bifunctional chelating agents DOTA and DTPA. Various parameters were studied: method of Acm purification, conditions of Acm conjugation, the method for determination of number of chelate agent coupled to the Acm, method for purification of the conjugated antibody Acm, conditions of labeling of the conjugated antibody with lutetium-177, method of purification of the radiolabeled immuno conjugate, method of radiochemical purity (RP), specific binding in vitro Raji cells (Human Burkitt) and biological distribution performed in normal Balb-c mouse. The three methodologies employed in pre-purification of Acm (dialysis, size exclusion chromatograph and dial filtration) demonstrated to be efficient; they provided sample recovery exceeding 90%. However, the methodology of dial filtration presents minimal sample loss, and gave the final recovery of the sample in micro liters; thereby facilitating sample use in subsequent experiments. Numbers of chelators attached to the Acm molecule was proportional to the molar ratio studied. When we evaluated the influence of different

  6. Antineutrophil Cytoplasmic Antibodies, Autoimmune Neutropenia, and Vasculitis

    Science.gov (United States)

    Grayson, Peter C.; Sloan, J. Mark; Niles, John L.; Monach, Paul A.; Merkel, Peter A.

    2011-01-01

    Objectives Reports of an association between antineutrophil cytoplasmic antibodies (ANCA) and autoimmune neutropenia have rarely included cases of proven vasculitis. A case of ANCA-associated vasculitis (AAV) with recurrent neutropenia is described and relevant literature on the association between ANCA, neutropenia, and vasculitis is reviewed. Methods Longitudinal clinical assessments and laboratory findings are described in a patient with AAV and recurrent episodes of profound neutropenia from December 2008 – October 2010. A PubMed database search of the medical literature was performed for papers published from 1960 through October 2010 to identify all reported cases of ANCA and neutropenia. Results A 49 year-old man developed recurrent neutropenia, periodic fevers, arthritis, biopsy-proven cutaneous vasculitis, sensorineural hearing loss, epididymitis, and positive tests for ANCA with specificity for antibodies to both proteinase 3 and myeloperoxidase. Antineutrophil membrane antibodies were detected during an acute neutropenic phase and were not detectable in a post-recovery sample, whereas ANCA titers did not seem to correlate with neutropenia. An association between ANCA and neutropenia has been reported in 74 cases from 24 studies in the context of drug/toxin exposure, underlying autoimmune disease, or chronic neutropenia without underlying autoimmune disease. In these cases, the presence of atypical ANCA patterns and other antibodies were common; however, vasculitis was uncommon and when it occurred was usually limited to the skin and in cases of underlying toxin exposure. Conclusions ANCA is associated with autoimmune neutropenia, but systemic vasculitis rarely occurs in association with ANCA and neutropenia. The interaction between neutrophils and ANCA may provide insight into understanding both autoimmune neutropenia and AAV. PMID:21507463

  7. High performance steam development. Final report, Phase No. 3: 1500{degree}F steam plant for industrial cogeneration prototype development tests

    Energy Technology Data Exchange (ETDEWEB)

    Duffy, T.; Schneider, P.

    1996-01-01

    As a key part of DOE`s and industry`s R&D efforts to improve the efficiency, cost, and emissions of power generation, a prototype High Performance Steam System (HPSS) has been designed, built, and demonstrated. The world`s highest temperature ASME Section I coded power plant successfully completed over 100 hours of development tests at 1500{degrees}F and 1500 psig on a 56,000 pound per hour steam generator, control valve and topping turbine at an output power of 5500 hp. This development advances the HPSS to 400{degrees}F higher steam temperature than the current best technology being installed around the world. Higher cycle temperatures produce higher conversion efficiencies and since steam is used to produce the large majority of the world`s power, the authors expect HPSS developments will have a major impact on electric power production and cogeneration in the twenty-first century. Coal fueled steam plants now produce the majority of the United States electric power. Cogeneration and reduced costs and availability of natural gas have now made gas turbines using Heat Recovery Steam Generators (HRSG`s) and combined cycles for cogeneration and power generation the lowest cost producer of electric power in the United States. These gas fueled combined cycles also have major benefits in reducing emissions while reducing the cost of electricity. Development of HPSS technology can significantly improve the efficiency of cogeneration, steam plants, and combined cycles. Figure 2 is a TS diagram that shows the HPSS has twice the energy available from each pound of steam when expanding from 1500{degrees}F and 1500 psia to 165 psia (150 psig, a common cogeneration process steam pressure). This report describes the prototype component and system design, and results of the 100-hour laboratory tests. The next phase of the program consists of building up the steam turbine into a generator set, and installing the power plant at an industrial site for extended operation.

  8. Final Project Report: DOE Award FG02-04ER25606 Overlay Transit Networking for Scalable, High Performance Data Communication across Heterogeneous Infrastructure

    Energy Technology Data Exchange (ETDEWEB)

    Beck, Micah; Moore, Terry

    2007-08-31

    As the flood of data associated with leading edge computational science continues to escalate, the challenge of supporting the distributed collaborations that are now characteristic of it becomes increasingly daunting. The chief obstacles to progress on this front lie less in the synchronous elements of collaboration, which have been reasonably well addressed by new global high performance networks, than in the asynchronous elements, where appropriate shared storage infrastructure seems to be lacking. The recent report from the Department of Energy on the emerging 'data management challenge' captures the multidimensional nature of this problem succinctly: Data inevitably needs to be buffered, for periods ranging from seconds to weeks, in order to be controlled as it moves through the distributed and collaborative research process. To meet the diverse and changing set of application needs that different research communities have, large amounts of non-archival storage are required for transitory buffering, and it needs to be widely dispersed, easily available, and configured to maximize flexibility of use. In today's grid fabric, however, massive storage is mostly concentrated in data centers, available only to those with user accounts and membership in the appropriate virtual organizations, allocated as if its usage were non-transitory, and encapsulated behind legacy interfaces that inhibit the flexibility of use and scheduling. This situation severely restricts the ability of application communities to access and schedule usable storage where and when they need to in order to make their workflow more productive. (p.69f) One possible strategy to deal with this problem lies in creating a storage infrastructure that can be universally shared because it provides only the most generic of asynchronous services. Different user communities then define higher level services as necessary to meet their needs. One model of such a service is a Storage Network

  9. Replacing reprogramming factors with antibodies selected from combinatorial antibody libraries.

    Science.gov (United States)

    Blanchard, Joel W; Xie, Jia; El-Mecharrafie, Nadja; Gross, Simon; Lee, Sohyon; Lerner, Richard A; Baldwin, Kristin K

    2017-10-01

    The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) is usually achieved by exogenous induction of transcription by factors acting in the nucleus. In contrast, during development, signaling pathways initiated at the membrane induce differentiation. The central idea of this study is to identify antibodies that can catalyze cellular de-differentiation and nuclear reprogramming by acting at the cell surface. We screen a lentiviral library encoding ∼100 million secreted and membrane-bound single-chain antibodies and identify antibodies that can replace either Sox2 and Myc (c-Myc) or Oct4 during reprogramming of mouse embryonic fibroblasts into iPSCs. We show that one Sox2-replacing antibody antagonizes the membrane-associated protein Basp1, thereby de-repressing nuclear factors WT1, Esrrb and Lin28a (Lin28) independent of Sox2. By manipulating this pathway, we identify three methods to generate iPSCs. Our results establish unbiased selection from autocrine combinatorial antibody libraries as a robust method to discover new biologics and uncover membrane-to-nucleus signaling pathways that regulate pluripotency and cell fate.

  10. Radioiodination of antibodies for tumor imaging

    International Nuclear Information System (INIS)

    Saha, G.B.

    1983-01-01

    In view of the great potential of radioiodinated antibody for the detection and treatment of cancer, the present article deals with the various techniques of radioiodination of antibody and their uses. Topics include methods of iodination of antibody, advantages and disadvantages of different methods, and effects of radioiodination on the antibody molecules with respect to their physiochemical and immunologic reactivity. In addition, the clinical usefulness of radioiodinated antibodies is discussed. (Auth.)

  11. Antibodies from plants for bionanomaterials.

    Science.gov (United States)

    Edgue, Gueven; Twyman, Richard M; Beiss, Veronique; Fischer, Rainer; Sack, Markus

    2017-11-01

    Antibodies are produced as part of the vertebrate adaptive immune response and are not naturally made by plants. However, antibody DNA sequences can be introduced into plants, and together with laboratory technologies that allow the design of antibodies recognizing any conceivable molecular structure, plants can be used as 'green factories' to produce any antibody at all. The advent of plant-based transient expression systems in particular allows the rapid, convenient, and safe production of antibodies, ranging from laboratory-scale expression to industrial-scale manufacturing. The key features of plant-based production include safety, speed, low cost, and convenience, allowing newcomers to rapidly master the technology and use it to its full advantage. Manufacturing in plants has recently achieved significant milestones and offers more than just an alternative to established microbial and mammalian cell platforms. The use of plants for product development in particular offers the power and flexibility to easily coexpress many different genes, allowing the plug-and-play construction of novel bionanomaterials, perfectly complementing existing approaches based on plant virus-like particles. As well as producing single antibodies for applications in medicine, agriculture, and industry, plants can be used to produce antibody-based supramolecular structures and scaffolds as a new generation of green bionanomaterials that promise a bright future based on clean and renewable nanotechnology applications. WIREs Nanomed Nanobiotechnol 2017, 9:e1462. doi: 10.1002/wnan.1462 For further resources related to this article, please visit the WIREs website. © 2017 The Authors. WIREs Nanomedicine and Nanobiotechnology published by Wiley Periodicals, Inc.

  12. Antibody-Directed Phototherapy (ADP

    Directory of Open Access Journals (Sweden)

    M. Adil Butt

    2013-04-01

    Full Text Available Photodynamic therapy (PDT is a clinically-approved but rather under-exploited treatment modality for cancer and pre-cancerous superficial lesions. It utilises a cold laser or LED to activate a photochemical reaction between a light activated drug (photosensitiser-drug and oxygen to generate cytotoxic oxygen species. These free radical species damage cellular components leading to cell death. Despite its benefits, the complexity, limited potency and side effects of PDT have led to poor general usage. However, the research area is very active with an increasing understanding of PDT-related cell biology, photophysics and significant progress in molecular targeting of disease. Monoclonal antibody therapy is maturing and the next wave of antibody therapies includes antibody-drug conjugates (ADCs, which promise to be more potent and curable. These developments could lift antibody-directed phototherapy (ADP to success. ADP promises to increase specificity and potency and improve drug pharmacokinetics, thus delivering better PDT drugs whilst retaining its other benefits. Whole antibody conjugates with first generation ADP-drugs displayed problems with aggregation, poor pharmacokinetics and loss of immuno-reactivity. However, these early ADP-drugs still showed improved selectivity and potency. Improved PS-drug chemistry and a variety of conjugation strategies have led to improved ADP-drugs with retained antibody and PS-drug function. More recently, recombinant antibody fragments have been used to deliver ADP-drugs with superior drug loading, more favourable pharmacokinetics, enhanced potency and target cell selectivity. These improvements offer a promise of better quality PDT drugs.

  13. Antibody Validation by Western Blotting.

    Science.gov (United States)

    Signore, Michele; Manganelli, Valeria; Hodge, Alex

    2017-01-01

    Validation of antibodies is an integral part of translational research, particularly for biomarker discovery. Assaying the specificity of the reagent (antibody) and confirming the identity of the protein biomarker is of critical importance prior to implementing any biomarker in clinical studies, and the lack of such quality control tests may result in unexpected and/or misleading results.Antibody validation is the procedure in which a single antibody is thoroughly assayed for sensitivity and specificity. Although a plethora of commercial antibodies exist, antibody specificity must be extensively demonstrated using diverse complex biological samples, rather than purified recombinant proteins, prior to use in clinical translational research. In the simplest iteration, antibody specificity is determined by the presence of a single band in a complex biological sample, at the expected molecular weight, on a Western blot.To date, numerous Western blotting procedures are available, based on either manual or automated systems and spanning the spectrum of single blots to multiplex blots. X-ray film is still employed in many research laboratories, but digital imaging has become a gold standard in immunoblotting. The basic principles of Western blotting are (a) separation of protein mixtures by gel electrophoresis, (b) transfer of the proteins to a blot, (c) probing the blot for a protein or proteins of interest, and (d) subsequent detection of the protein by chemiluminescent, fluorescent, or colorimetric methods. This chapter focuses on the chemiluminescent detection of proteins using a manual Western blotting system and a vacuum-enhanced detection system (SNAP i.d.™, Millipore).

  14. An effective intracellular delivery system of monoclonal antibody for treatment of tumors: erythrocyte membrane-coated self-associated antibody nanoparticles

    Science.gov (United States)

    Gao, Lipeng; Han, Lin; Ding, Xiaoling; Xu, Jiaojiao; Wang, Jing; Zhu, Jianzhong; Lu, Weiyue; Sun, Jihong; Yu, Lei; Yan, Zhiqiang; Wang, Yiting

    2017-08-01

    Antibody-based drugs have attracted much attention for their targeting ability, high efficacy and low toxicity. But it is difficult for those intrabodies, a kind of antibody whose targets are intracellular biomarkers, to become effective drugs due to the lack of intracellular delivery strategy and their short circulation time in blood. Human telomerase reverse transcriptase (hTERT), an important biomarker for tumors, is expressed only in cytoplasm instead of on cell membrane. In this study, the anti-hTERT blocking monoclonal antibody (mAb), as the model intrabody, was used to prepare nanoparticles (NPs), followed by the encapsulation of erythrocyte membrane (EM), to obtain the EM-coated anti-hTERT mAb NPs delivery system. The final NPs showed a z-average hydrodynamic diameter of about 197.3 nm. The in vitro cellular uptake by HeLa cells confirmed that compared with free anti-hTERT mAb, the EM-coated anti-hTERT mAb NPs exhibited a significantly increased uptake by tumor cells. Besides, the pharmacokinetic study confirmed that the EM encapsulation can remarkably prolong the circulation time and increase the area under curve (AUC) of NPs in blood. The EM-coated anti-hTERT mAb NPs exhibited a remarkably decreased uptake by macrophages than uncoated NPs, which may be responsible for the prolonged circulation time and increased AUC. Furthermore, the frozen section of tumor tissue was performed and proved that the EM-coated anti-hTERT mAb NPs can be more effectively accumulated in tumor tissues than the free mAb and uncoated NPs. In summary, this study indicated that EM-coated anti-hTERT mAb NPs are an effective delivery system for the long circulation and intracellular delivery of an intrabody, and make it possible for the intracellular biomarkers to become the potential targets of drugs.

  15. Synergy between vascular targeting agents and antibody-directed therapy

    International Nuclear Information System (INIS)

    Pedley, R. Barbara; El-Emir, Ethaar; Flynn, Aiden A.; Boxer, Geoffrey M.; Dearling, Jason; Raleigh, James A.; Hill, Sally A.; Stuart, Sam; Motha, Reeya; Begent, Richard H.J.

    2002-01-01

    Purpose: Tumor heterogeneity necessitates the use of combined therapies. We have shown that combining antibody-directed therapy with antivascular agents converts a subcurative to a curative treatment. The purpose of this study was to investigate, by radioluminographic and microscopic techniques, the regional effects of the two complementary therapies. Methods and Materials: Nude mice bearing colorectal tumors were injected with 125 I-labeled anti-carcinoembryonic antigen antibody, and images were obtained for antibody distribution and modeling studies using radioluminography. For therapy studies, the mice were given radioimmunotherapy alone ( 131 I-A5B7 anti-carcinoembryonic antigen antibody), the antivascular agent combretastatin A-4 3-0-phosphate (200 mg/kg), or both. Extra mice were used to study the regional tumor effects of these therapies over time: relevant histochemical procedures were performed on tissue sections to obtain composite digital microscopic images of apoptosis, blood vessels, perfusion, hypoxia, and morphology. Results: Antibody distribution, modeling, and immunohistochemistry showed how radioimmunotherapy (7.4 MBq/40 μg antibody) effectively treated the outer, well-oxygenated tumor region only. Combretastatin A-4 3-0-phosphate treated the more hypoxic center, and in doing so altered the relationship between tumor parameters. Conclusion: The combined complementary therapies produced cures by destroying tumor regions with different pathophysiologies. Relating these regional therapeutic effects to the relevant tumor parameters microscopically allows optimization of therapy and improved translation to clinical trials

  16. Arenavirus Glycan Shield Promotes Neutralizing Antibody Evasion and Protracted Infection.

    Directory of Open Access Journals (Sweden)

    Rami Sommerstein

    2015-11-01

    Full Text Available Arenaviruses such as Lassa virus (LASV can cause severe hemorrhagic fever in humans. As a major impediment to vaccine development, delayed and weak neutralizing antibody (nAb responses represent a unifying characteristic of both natural infection and all vaccine candidates tested to date. To investigate the mechanisms underlying arenavirus nAb evasion we engineered several arenavirus envelope-chimeric viruses and glycan-deficient variants thereof. We performed neutralization tests with sera from experimentally infected mice and from LASV-convalescent human patients. NAb response kinetics in mice correlated inversely with the N-linked glycan density in the arenavirus envelope protein's globular head. Additionally and most intriguingly, infection with fully glycosylated viruses elicited antibodies, which neutralized predominantly their glycan-deficient variants, both in mice and humans. Binding studies with monoclonal antibodies indicated that envelope glycans reduced nAb on-rate, occupancy and thereby counteracted virus neutralization. In infected mice, the envelope glycan shield promoted protracted viral infection by preventing its timely elimination by the ensuing antibody response. Thus, arenavirus envelope glycosylation impairs the protective efficacy rather than the induction of nAbs, and thereby prevents efficient antibody-mediated virus control. This immune evasion mechanism imposes limitations on antibody-based vaccination and convalescent serum therapy.

  17. Arenavirus Glycan Shield Promotes Neutralizing Antibody Evasion and Protracted Infection

    Science.gov (United States)

    Malinge, Pauline; Magistrelli, Giovanni; Fischer, Nicolas; Sahin, Mehmet; Bergthaler, Andreas; Igonet, Sebastien; ter Meulen, Jan; Rigo, Dorothée; Meda, Paolo; Rabah, Nadia; Coutard, Bruno; Bowden, Thomas A.; Lambert, Paul-Henri; Siegrist, Claire-Anne; Pinschewer, Daniel D.

    2015-01-01

    Arenaviruses such as Lassa virus (LASV) can cause severe hemorrhagic fever in humans. As a major impediment to vaccine development, delayed and weak neutralizing antibody (nAb) responses represent a unifying characteristic of both natural infection and all vaccine candidates tested to date. To investigate the mechanisms underlying arenavirus nAb evasion we engineered several arenavirus envelope-chimeric viruses and glycan-deficient variants thereof. We performed neutralization tests with sera from experimentally infected mice and from LASV-convalescent human patients. NAb response kinetics in mice correlated inversely with the N-linked glycan density in the arenavirus envelope protein’s globular head. Additionally and most intriguingly, infection with fully glycosylated viruses elicited antibodies, which neutralized predominantly their glycan-deficient variants, both in mice and humans. Binding studies with monoclonal antibodies indicated that envelope glycans reduced nAb on-rate, occupancy and thereby counteracted virus neutralization. In infected mice, the envelope glycan shield promoted protracted viral infection by preventing its timely elimination by the ensuing antibody response. Thus, arenavirus envelope glycosylation impairs the protective efficacy rather than the induction of nAbs, and thereby prevents efficient antibody-mediated virus control. This immune evasion mechanism imposes limitations on antibody-based vaccination and convalescent serum therapy. PMID:26587982

  18. Biomimetic small peptide functionalized affinity monoliths for monoclonal antibody purification.

    Science.gov (United States)

    Wang, Xiangyu; Xia, Donghai; Han, Hai; Peng, Kun; Zhu, Peijie; Crommen, Jacques; Wang, Qiqin; Jiang, Zhengjin

    2018-08-09

    The rapid development of monoclonal antibodies (mAbs) in therapeutic and diagnostic applications has necessitated the advancement of mAbs purification technologies. In this study, a biomimetic small peptide ligand 3,5-di-tert-butyl-4-hydroxybenzoic acid-Arg-Arg-Gly (DAAG) functionalized monolith was fabricated through a metal ion chelation-based multi-step approach. The resulting monolith showed good chromatographic performance. Compared with the Ni 2+ based IMAC monolith, the DAAG functionalized monolith exhibited not only excellent specificity but also higher dynamic binding capacity (DBC). The 10% DBC and 50% DBC for hIgG reached as high values as 26.0 and 34.6 mg/mL, respectively, at a ligand density of 8.8 μmol/mL, due to the high porosity and accessibility of the monolithic matrix. Moreover, the stability of the DAAG functionalized monolith in successive breakthrough experiments indicates that it has a promising potential for long-term use in mAbs purification. Finally, the DAAG functionalized monolith was successfully applied to the purification of trastuzumab or human immunoglobulin G (hIgG) from biological samples. Copyright © 2018 Elsevier B.V. All rights reserved.

  19. SOL-PAC: Integrated solar heating and heat pump systems for refurbished buildings - Performance analysis; SOL-PAC: analyse des performances du couplage d'une pompe a chaleur avec une installation solaire thermique pour la renovation - Rapport final

    Energy Technology Data Exchange (ETDEWEB)

    Citherlet, C.; Bony, J.; Nguyen, B.

    2008-07-01

    The purpose of this study is to evaluate the energetic and economic performances of various types of brine/water heat pumps coupled with a fan coil fitted with solar collectors. Different indicators such as the annual coefficient of performance or the electricity consumption have been compared between the different analysed systems. The optimization of the energy control system and energy storage was investigated in order to reduce the operation time of the fan coil. This study has been performed using the TRNSYS computerized simulation package. The icing of the fan coil as well as its defrosting cycles could not been taken into account within the framework of this project, because of a lack of a numerical model. At current electricity prices, the most economical solution remains the heat pump without solar collectors. However, from an energy point of view, the annual overall coefficient of performance of the installation may be improved by 15 to 36%, depending on the climate, thanks to the coupling with solar collectors. (author)

  20. Improving food and agricultural production. Thailand. Application on monoclonal antibodies for progesterone measurement

    International Nuclear Information System (INIS)

    Butcher, G.W.

    1991-01-01

    The duties of the mission were to provide instructions on the maintenance of hybridoma cell lines and their culture and the harvesting of monoclonal antibodies; to assist the counterparts in Thailand to develop work plans for the use of monoclonal antibodies in radioimmunoassay measurements of progesterone; and to assess the need for and feasibility of establishing a laboratory for producing monoclonal antibodies directed against progesterone. The report contains a summary of the activities performed in fulfillment of these duties

  1. Seroprevalence of Leptospira antibodies among populations at risk

    Directory of Open Access Journals (Sweden)

    Abdul-Baki Abdullah Al-Robasi

    2015-03-01

    Full Text Available Objective: This study was performed to assess the Leptospira IgG antibodies seroprevalence among populations at risk in Hodeida Governorate, Yemen. Methods: A total of 200 subjects (136 males and 64 females participated in this study during June and December 2012.They represented 10 sewage workers, 22 butchers, 16 construction workers, 108 agriculture workers, 20 hospital sanitary workers and 24 blood donors. Predesigned questionnaires and consent were taken from each individual. Blood samples were collected from subjects, and the sera were tested by ELISA to detect the presence of leptospira IgG antibodies. The possible related factors for seropositivity were evaluated. Results: Leptospira IgG antibodies were found positive in 42% of the participants. The highest seroprevalence level was detected in sewage workers (80%, followed by hospital sanitary workers (60%, construction workers (37.5% and farmers (37%. The lowest of antibodies was in butchers (36.4%. Seroprevalence among blood donors was 25% which was comparatively less than of the populations at risk. Seropositivity of Leptospira IgG antibodies was found higher among males than females (42.6% vs. 34.4%. The highest Leptospira antibodies seropositivity was among elderly participants (81.8%. The seropositivity of antibodies in population live in rural and urban areas was not significant differences. As for closely contacting with animals, the highest antibodies were discovered in people who had goats (80% and sheep (60.9%. Conclusion: Individuals engaged in risk activities are often exposed to leptospiral infection. Therefore, control and prevention policy toward these people are necessary. J Microbiol Infect Dis 2015;5(1: 1-4

  2. Antibody orientation on biosensor surfaces: a minireview.

    Science.gov (United States)

    Trilling, Anke K; Beekwilder, Jules; Zuilhof, Han

    2013-03-21

    Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains - the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.

  3. Incomplete Antibodies May Reduce ABO Cross-Match Incompatibility: A Pilot Study

    Directory of Open Access Journals (Sweden)

    Mehmet Özen

    2018-02-01

    Full Text Available Objective: Any erythrocyte transfusion among humans having type A or B blood groups is impossible due to antibodies causing fatal transfusion complications. A cross-match test is performed to prevent immune transfusion complications before transfusion. Our hypothesis is that the fragment antibody (Fab part of the antibody (incomplete antibody may be used to prevent an immune stimulus related to the complete antibody. Therefore, we designed a pilot study to evaluate the effectiveness of these incomplete antibodies using cross-match tests. Materials and Methods: Pepsin enzyme and staphylococcal protein A columns were used to cut anti-A and anti-B monoclonal antibodies and purify their Fab (2 fragments, respectively. An Rh-positive erythrocyte suspension with purified anti-A Fab (2 solution and B Rh-positive erythrocyte suspension with purified anti-B Fab (2 solution were combined correspondingly. Cross-match tests were performed by tube and gel centrifugation methods. The agglutination levels due to the anti-A and anti-B Fab (2 antibodies and their effects on the agglutination normally observed with complete antibodies were then measured. Results: No agglutination for the purified incomplete anti-A Fab (2 with A Rh+ erythrocyte and anti-B Fab (2 with B Rh+ erythrocyte combinations was observed in the tube cross-match tests. These agglutination levels were 1+ in two wells in the gel centrifugation cross-match tests. Fab (2-treated erythrocytes were also resistant to the agglutination that normally occurs with complete antibodies. Conclusion: We determined that the Fab (2 fragments of antibodies may not only be used to obtain a mild or negative reaction when compared to complete antibodies, but they might also be used for decreasing ABO incompatibility. Incomplete antibodies might be a therapeutic option in autoimmune hemolytic anemia and they may also be used in solid organ or hematopoietic stem cell transplantation. Therefore, we have planned an

  4. Adsorption orientations and immunological recognition of antibodies on graphene

    Science.gov (United States)

    Vilhena, J. G.; Dumitru, A. C.; Herruzo, Elena T.; Mendieta-Moreno, Jesús I.; Garcia, Ricardo; Serena, P. A.; Pérez, Rubén

    2016-07-01

    Large-scale molecular dynamics (MD) simulations and atomic force microscopy (AFM) in liquid are combined to characterize the adsorption of Immunoglobulin G (IgG) antibodies over a hydrophobic surface modeled with a three-layer graphene slab. We consider explicitly the water solvent, simulating systems with massive sizes (up to 770 000 atoms), for four different adsorption orientations. Protocols based on steered MD to speed up the protein diffusion stage and to enhance the dehydration process are combined with long simulation times (>150 ns) in order to make sure that the final adsorption states correspond to actual stable configurations. Our MD results and the AFM images demonstrate that the IgG antibodies are strongly adsorbed, do not unfold, and retain their secondary and tertiary structures upon deposition. Statistical analysis of the AFM images shows that many of the antibodies adopt vertical orientations, even at very small coverages, which expose at least one Fab binding site for recognition events. Single molecule force spectroscopy experiments demonstrate the immunological response of the deposited antibodies by recognizing its specific antigens. The above properties together with the strong anchoring and preservation of the secondary structure, make graphene an excellent candidate for the development of immunosensors.Large-scale molecular dynamics (MD) simulations and atomic force microscopy (AFM) in liquid are combined to characterize the adsorption of Immunoglobulin G (IgG) antibodies over a hydrophobic surface modeled with a three-layer graphene slab. We consider explicitly the water solvent, simulating systems with massive sizes (up to 770 000 atoms), for four different adsorption orientations. Protocols based on steered MD to speed up the protein diffusion stage and to enhance the dehydration process are combined with long simulation times (>150 ns) in order to make sure that the final adsorption states correspond to actual stable configurations. Our

  5. Autoimmune thyroiditis in antinuclear antibody positive children without rheumatologic disease

    Directory of Open Access Journals (Sweden)

    Arkachaisri Thaschawee

    2010-05-01

    Full Text Available Abstract Background Children are commonly referred to a pediatric rheumatology center for the laboratory finding of an Anti-nuclear antibody (ANA of undetermined significance. Previous studies regarding adult rheumatology patients have supported an association between ANA and anti-thyroid antibodies, with the prevalence of thyroid antibodies being significantly higher in patients referred to a rheumatology center for an ANA without evidence of connective tissue disease compared to the general population. The purpose of the present study was to determine the frequency of thyroid antibodies in children referred to a pediatric rheumatology center for a positive ANA without evidence of a connective tissue disease. Methods A retrospective chart review was performed on children who were referred to our pediatric rheumatology center between August 2003 and March 2007 for positive ANA with concurrent thyroid antibody and thyroid function tests performed who did not fulfill criteria for a specific connective tissue disease. Laboratory and clinical features were recorded and analyzed. Mean and standard deviation were used to describe continuous data. Chi-square or Fisher's exact tests were used to compare proportions between variables. Results One-hundred and four ANA-positive patients with concurrent thyroid studies were evaluated (88% female, 93% Caucasian, mean age 11.9 ± 4.0 years. Half of patients had an ANA titer ≥ 1:320. The ANA pattern was speckled in 60% of the patients. Thyroid antibodies were detected in 30% of the patients. Anti-Thyroglobulin (ATG was detected in 29% and Anti-thyroid peroxidase (ATPO in 21% of the patients; of these children, 14% had hypothyroidism. ANA pattern and titer were not associated with anti-thyroid antibody positivity. Conclusion Thyroid antibodies associated with chronic lymphocytic thyroiditis, ATG and ATPO, were detected significantly higher in ANA-positive children without a rheumatologic condition (30% as

  6. INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY

    Directory of Open Access Journals (Sweden)

    O.V. Galkin

    2017-10-01

    Full Text Available Obtaining single-chain variable fragments (scFv of recombinant antibodies in E. coli cells is often associated with numerous problems causing low yields or inactive conformation of the product. The aim of this work was to study the influence of staphylococcal protein A fragment fused with scFv antibodies (SpA-tag on the efficiency of expression of final product. Examination of scFv antibodies of different origin and specificity has shown that in similar expression systems fused scFv is synthesized in much higher quantities than free scFv. Furthermore, the scFv antibodies in fused form retained their antigen-binding properties and the SpA fragment the ability to bind other immunoglobulins. Thus, the proposed strategy can be considered effective in improving the efficiency of scFv-antibodies production in E. coli cells.

  7. Prometheus Project final report

    Science.gov (United States)

    Taylor, Randall

    2005-01-01

    This Final Report serves as an executive summary of the Prometheus Project's activities and deliverables from November 2002 through September 2005. It focuses on the challenges from a technical and management perspective, what was different and innovative about this project, and identifies the major options, decisions, and accomplishments of the Project team as a whole. However, the details of the activities performed by DOE NR and its contractors will be documented separately in accordance with closeout requirements of the DOE NR and consistent with agreements between NASA and NR.

  8. B280 Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Ulmer, J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Loomis, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Sampson, M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2011-09-29

    Lawrence Livermore National Security, LLC (LLNS) has commissioned this independent Structural Condition Assessment as part of its Functional Management Review of the decommissioned Livermore Pool-Type Reactor (LPTR) located in Building 280 at Lawrence Livermore National laboratory (LLNL) for the purpose of addressing a potential management concern regarding the nature and impact of observed cracks in the LPTR shielding structure discovered approximately 8 months earlier. This assessment represents the final report from an initial investigation performed between July 11th and July 15th, 2011. The Exit Briefing presented by the review team at the conclusion of the on-site investigation phase is included as Attachment A.

  9. Final performance report for Project JEM

    Energy Technology Data Exchange (ETDEWEB)

    McKinney, M.J.; Jenkins, S.

    1997-12-31

    Project JEM (Jarvis Enhancement of Males) is a pre-college program directed toward stimulating disadvantaged, talented African American males in grades four, five, and six to attend college and major in mathematics, science, computer science, or related technical areas needed by the US Department of Energy. Twenty young African American male students were recruited from Gladewater Independent School District (ISD), Longview ISD, Hawkins ISD, Tyler ISD, Winona ISD and big Sandy ISD. Students enrolled in the program range from ages 10 to 13 and are in grades four, five and six. Student participants in the 1997 Project JEM Program attended Saturday Academy sessions and a four week intensive, summer residential program. The information here provides a synopsis of the activities which were conducted through each program component.

  10. Trinidad Lake asphalt overlay performance : final report.

    Science.gov (United States)

    2014-07-01

    Construction of the new Tacoma Narrows Bridge (TNB) included a steel orthotropic bridge deck. : The higher flexibility of an orthotropic deck causes pavement placed upon it to fatigue and crack more : quickly than pavement placed on a normal roadway....

  11. (WINDPEP Project performance report). Final report

    Energy Technology Data Exchange (ETDEWEB)

    Speelmon, J.L.

    1983-06-28

    The goals and results of the WINDPEP project are presented. The project promoted the use of wind generators to produce electricity for residences and small businesses located in Federal Region X. (BCS)

  12. Antibody-protein A conjugated quantum dots for multiplexed imaging of surface receptors in living cells.

    Science.gov (United States)

    Jin, Takashi; Tiwari, Dhermendra K; Tanaka, Shin-Ichi; Inouye, Yasushi; Yoshizawa, Keiko; Watanabe, Tomonobu M

    2010-11-01

    To use quantum dots (QDs) as fluorescent probes for receptor imaging, QD surface should be modified with biomolecules such as antibodies, peptides, carbohydrates, and small-molecule ligands for receptors. Among these QDs, antibody conjugated QDs are the most promising fluorescent probes. There are many kinds of coupling reactions that can be used for preparing antibody conjugated QDs. Most of the antibody coupling reactions, however, are non-selective and time-consuming. In this paper, we report a facile method for preparing antibody conjugated QDs for surface receptor imaging. We used ProteinA as an adaptor protein for binding of antibody to QDs. By using ProteinA conjugated QDs, various types of antibodies are easily attached to the surface of the QDs via non-covalent binding between the F(c) (fragment crystallization) region of antibody and ProteinA. To show the utility of ProteinA conjugated QDs, HER2 (anti-human epidermal growth factor receptor 2) in KPL-4 human breast cancer cells were stained by using anti-HER2 antibody conjugated ProteinA-QDs. In addition, multiplexed imaging of HER2 and CXCR4 (chemokine receptor) in the KPL-4 cells was performed. The result showed that CXCR4 receptors coexist with HER2 receptors in the membrane surface of KPL-4 cells. ProteinA mediated antibody conjugation to QDs is very useful to prepare fluorescent probes for multiplexed imaging of surface receptors in living cells.

  13. Antibody profiling sensitivity through increased reporter antibody layering

    Energy Technology Data Exchange (ETDEWEB)

    Apel, William A; Thompson, Vicki S

    2013-02-26

    A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

  14. Antibody profiling sensitivity through increased reporter antibody layering

    International Nuclear Information System (INIS)

    Apel, William A.; Thompson, Vickie S.

    2013-01-01

    A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

  15. Antibody profiling sensitivity through increased reporter antibody layering

    Energy Technology Data Exchange (ETDEWEB)

    Apel, William A.; Thompson, Vicki S.

    2013-02-26

    A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

  16. Antibody profiling sensitivity through increased reporter antibody layering

    Energy Technology Data Exchange (ETDEWEB)

    Apel, William A.; Thompson, Vicki S

    2010-04-13

    A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

  17. Antibody profiling sensitivity through increased reporter antibody layering

    Energy Technology Data Exchange (ETDEWEB)

    Apel, William A.; Thompson, Vicki S.

    2017-03-28

    A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

  18. Production and Characterization of HCG Poly clonal Antibodies

    International Nuclear Information System (INIS)

    Moustafa, K.A.

    2009-01-01

    To prepare a radioimmunoassay system for measuring hcg hormone, the anti-hcg polyclonal antibodies must be firstly prepared. The present study was aimed to produce and characterize the anti-hcg polyclonal antibodies. The anti hcg polyclonal Antibes which were produced by immunizing five Balb/c mice with a highly purified β-hcg hormone. A complete Freunds adjuvant was used for the first injection while incomplete Freunds adjuvant was used for the following boosters. Blood spots were obtained from the immunized mice and tested for the presence of antibodies. At the end of the immunization schedule, the mice was exposed to anesthesia using ether then dissected and the blood samples were collected from the heart. The serum (antisera) was separated from the blood containing anti-hcg polyclonal antibodies. The produced antibodies were purified then immobilized onto the surface of magnetic particles to prepare the solid phase anti-hcg magnetic particles. The characterization parameters include accuracy, parallelism, sensitivity, specificity and comparison studies between the prepared system and the commercial used one, were performed. Also, the labeling of hcg hormone with 1251 was performed using chloramine-T method. The obtained results indicated that the produced solid phase of polyclonal anti-hcg for measuring the hcg hormone was good, accurate, sensitive, specific and highly correlated with that of the commercial method

  19. Hu and Yo antibodies have heterogeneous avidity.

    Science.gov (United States)

    Totland, Cecilie; Aarseth, Jan; Vedeler, Christian

    2007-04-01

    Onconeural antibodies such as anti-Hu and anti-Yo may be important in the pathogenesis of paraneoplastic neurological syndromes. The avidity of these antibodies is not known. In this study, we compared the avidity of Hu and Yo antibodies both at single time points and over a time range of 2 months to 6 years. The avidity of Yo and Hu antibodies differed among the patients, but anti-Yo generally had higher avidity than anti-Hu. Whether Yo antibodies are more pathogenic than Hu antibodies are presently unknown.

  20. Suppression of the immune response to ovalbumin in vivo by anti-idiotypic antibodies

    International Nuclear Information System (INIS)

    Grinevich, A.S.; Pinegin, B.V.

    1986-01-01

    Conditions of suppression of the immune response to a food allergin (ovalbumin) were studied with the aid of anti-idiotypic (AID) antibodies. Hen ovalbumin was used and the experiments were performed on mice. Antibodies were isolated from the resulting protein fractions and tested for inhibitor activity by the method of direct radioimmunologic analysis. The test system consisted of the reaction of binding the globulin fraction to the total preparation of antibodies to ovalbumin from mice and a 125 I-labeled total preparation of antibodies to ovalbumin of the same animals

  1. A Comparison of Anti-Nuclear Antibody Quantification Using Automated Enzyme Immunoassays and Immunofluorescence Assays

    DEFF Research Database (Denmark)

    Baronaite, Renata; Engelhart, Merete; Mørk Hansen, Troels

    2014-01-01

    using IFA and automated EIA techniques. The IFA results generated by two independent laboratories were compared with the EIA results from antibodies against double-stranded DNA (dsDNA), from ANA screening, and from tests of the seven included subantigens. The final IFA and EIA results for 386 unique......, with Cohen's kappa value of 0.30 (95% confidence interval (CI) = 0.14-0.46), which decreased to 0.23 (95% CI = 0.06-0.40) when the results for dsDNA were omitted. The EIA method was less reliable for assessing nuclear and speckled reactivity patterns, whereas the IFA method presented difficulties detecting...... dsDNA and Ro activity. The automated EIA method was performed in a similar way to the conventional IFA method using HEp-2 cells; thus, automated EIA may be used as a screening test....

  2. The study of CP violation in the B0 → D+D- by means of the BABAR detector. Measurement of the performances of DIRC Cherenkov detector of BABAR: Prototype-II and final detector

    International Nuclear Information System (INIS)

    Benkebil, Mehdi

    1999-01-01

    The work presented in this thesis is divided into two parts: the physics analysis of the decay mode B 0 → D + D - and the performance obtained with a new type of a particle identification detector using the Cherenkov effect technique: the DIRC. The analysis of this decay mode has been performed with data generated from fast simulation and a preliminary version of the reconstruction program. The branching ratio of this channel is predicted to be 4.5 x 10 -4 . The uncertainty in the sin 2 β measurement obtained with this mode is: σ(sin 2β)0.19 and 0.32 for fast simulation and preliminary version of the reconstruction program, respectively. The comparison of this result with the one obtained in the B 0 → J/ψK s 0 mode will bring very useful theoretical insights. The performance study of the DIRC has been done on the prototype-II and the final detector. The beam-test results in terms resolution on the θ c angle and number of Cherenkov photons are the following: σ(θ c ) = 10.2 ± 0.1 mrad per photon, σ(θ c ) = 3.2 ± 0.2 mrad per track and N γ 15.7 ± 0.1 at θ dip = 20 angle and 0 transmission in the bar. The analysis of the first cosmic data collected by the BABAR detector has allowed to study the DIRC in its final configuration. Among all the results obtained, we give the following ones: σ(θ c ) = 10.09 ± 0.06 mrad per photon, σ(θ c ) = 4.71 ± 0.14 mrad per track and N γ 35.2 ± 3.8 at θ dip = 20 angle and 0 transmission in the bar. The extrapolation to the real condition of BABAR for all these results shows that the DIRC will run with performances similar to the nominal values. A detailed study of the background shows that, even though it will not be negligible, it will not compromise the DIRC performances in BABAR. (author)

  3. 9 CFR 113.452 - Erysipelothrix Rhusiopathiae Antibody.

    Science.gov (United States)

    2010-01-01

    ..., DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS STANDARD... Antibody is a specific antibody product containing antibodies directed against one or more somatic antigens...

  4. Performance evaluation of the Bio-Rad Laboratories GS HIV Combo Ag/Ab EIA, a 4th generation HIV assay for the simultaneous detection of HIV p24 antigen and antibodies to HIV-1 (groups M and O) and HIV-2 in human serum or plasma.

    Science.gov (United States)

    Bentsen, Christopher; McLaughlin, Lisa; Mitchell, Elizabeth; Ferrera, Carol; Liska, Sally; Myers, Robert; Peel, Sheila; Swenson, Paul; Gadelle, Stephane; Shriver, M Kathleen

    2011-12-01

    A multi-center study was conducted to evaluate the Bio-Rad GS HIV Combo Ag/Ab EIA, a 4th generation HIV-1/HIV-2 assay for the simultaneous detection of HIV p24 antigen and antibodies to HIV-1 (groups M and O) and HIV-2 in human serum or plasma in adult and pediatric populations. The objectives of the study were to assess assay performance for the detection of acute HIV infections; sensitivity in known HIV positive samples; percent agreement with HIV status; specificity in low and high risk individuals of unknown HIV status; and to compare assay performance to a 3rd generation HIV assay. The evaluation included testing 9150 samples at four U.S. clinical trial sites, using three kit lots. Unlinked samples were from routine testing, repositories or purchased from vendors. GS HIV Combo Ag/Ab EIA detection in samples from individuals in two separate populations with acute HIV infection was 95.2% (20/21) and 86.4% (38/44). Sensitivity was 100% (1603/1603) in known antibody positive [HIV-1 Groups M and O, and HIV-2] samples. HIV p24 antigen detection was 100% (53/53) in HIV-1 culture supernatants. HIV-1 seroconversion panel detection improved by a range of 0-20 days compared to a 3rd generation HIV test. Specificity was 99.9% (5989/5996) in low risk, 99.9% (959/960) in high risk and 100% (100/100) in pediatric populations. The GS HIV Combo Ag/Ab EIA significantly reduced the diagnostic window when compared to the 3rd generation screening assay, enabling earlier diagnosis of HIV infection. The performance parameters of the Bio-Rad GS HIV Combo Ag/Ab EIA are well suited for use in HIV diagnostic settings. Copyright © 2011 Elsevier B.V. All rights reserved.

  5. Monoclonal antibodies to Treponema Pallidum.

    NARCIS (Netherlands)

    H.J.M. van de Donk; J.D.A. van Embden; M.F. van Olderen; A.D.M.E. Osterhaus (Albert); J.C. de Jong (Jan)

    1984-01-01

    textabstractThree successive fusions of mouse myeloma cells and spleen lymphocytes of a mouse immunized with Treponema Pallidum resulted in one hybridoma producing anti T. pallidum antibodies for each fusion. The mice were immunized with live pallidum cells respectively 1, 3 and 5 months before

  6. Preparation of an epitope-imprinted polymer with antibody-like selectivity for beta2-microglobulin and application in serum sample analysis with a facile method of on-line solid-phase extraction coupling with high performance liquid chromatography.

    Science.gov (United States)

    Yang, Fangfang; Deng, Dandan; Dong, Xiangchao; Lin, Shen

    2017-04-21

    Molecularly imprinted polymers (MIPs) for protein recognition have great application potential in the biological analysis. However, preparation of protein imprinted polymer is still facing challenge. Beta2-microglobulin (β 2 m) is a protein biomarker that can be used in diagnosis of different diseases. In this research, a novel MIP with ability of β 2 m recognition has been developed by epitope and surface-confined imprinting approaches. A peptide with sequence of MIQRTPKIQ was selected as template. A strategy of combination of hierarchical imprinting and template immobilization was employed in the β 2 m-MIP synthesis. Imprinted binding sites with open-entrance have been created that have good accessibility for β 2 m and facilitated fast reversible binding kinetics. The experimental results demonstrated that the MIP has good selectivity. It can differentiate the template from peptide with different sequence and distinguish the β 2 m from other proteins with similar size and pI values. After binding property study of the β 2 m-MIP, a method of β 2 m determination in serum was established in which β 2 m was on-line extracted by MIP and analyzed by HPLC process. The recoveries for spiked serum was ≥83% with RSD <1.1%, indicating that the method has good accuracy and precisions. The LOD and LOQ were 0.058 and 0.195mgL -1 respectively, which meet the requirements of the β 2 m analysis. The successful application of the β 2 m-MIP demonstrated that β 2 m has reversible binding on the MIP with a kinetics that can meet the requirements of the HPLC analysis. It also indicated that the β 2 m-MIP has good mechanical strength and reusability that can be applied reliably in the practical analysis. As a synthetic antibody, β 2 m-MIP is advantageous compared to the biological molecules. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Auto-antibodies and their association with clinical findings in women diagnosed with microscopic colitis.

    Directory of Open Access Journals (Sweden)

    Bodil Roth

    Full Text Available BACKGROUND: Microscopic colitis (MC is a disease manifested by diarrhoea and is divided into collagenous and lymphocytic colitis. The aetiology is unknown, but auto-immunity is suggested. Auto-antibodies have been only rarely examined in this entity. The aim of the study was to examine the prevalence of auto-antibodies, and to examine associations between the presence of antibodies and clinical findings. METHODS AND FINDINGS: Women with MC verified by biopsy and younger than 73 years, at any Department of Gastroenterology, in the district of Skåne, between 2002 and 2010 were invited to participate in this study. The patients were asked to complete both a questionnaire describing their medical history and the Gastrointestinal Symptom Rating Scale (GSRS. Blood samples were collected. Anti-nuclear antibodies (ANA, anti-neutrophil cytoplasmic antibodies (ANCA, anti-Saccharomyces cerevisiae antibodies (ASCA, and antibodies against glutamic acid decarboxylase (anti-GAD, islet antigens-like insulin 2 (anti-IA2, thyroid peroxidase (anti-TPO, and thyrotropin receptor (TRAK were analysed. Of 240 women identified, 133 were finally included in the study, median age 63 (59-67 years. Apart from the MC diagnosis, 52% also suffered from irritable bowel syndrome, 31% from hypertension and 31% from allergy. The prevalence of ANA (14%, ASCA IgG (13%, and anti-TPO antibodies (14% for these patients was slightly higher than for the general population, and were found together with other concomitant diseases. Patients had more of all gastrointestinal symptoms compared with norm values, irrespective of antibody expression. CONCLUSIONS: Women with MC have a slightly increased prevalence of some auto-antibodies. These antibodies are not associated with symptoms, but are expressed in patients with concomitant diseases, obscuring the pathophysiology and clinical picture of MC.

  8. A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method

    Directory of Open Access Journals (Sweden)

    Yuan-Cheng Cao

    2015-01-01

    Full Text Available This paper describes a combined antigen/antibody immunoassay implemented in a 96-well plate using fluorescent spectroscopic method. First, goat anti-human IgG was used to capture human IgG (model antigen; goat anti-human IgG (Cy3 or FITC was used to detect the model antigen; a saturating level of model antigen was then added followed by unlabelled goat anti-human IgG (model antibody; finally, Cy3 labelled rabbit anti-goat IgG was used to detect the model antibody. Two approaches were applied to the concomitant assay to analyze the feasibility. The first approach applied FITC and Cy3 when both targets were present at the same time, resulting in 50 ng/mL of the antibody detection limit and 10 ng/mL of antigen detection limit in the quantitative measurements of target concentration, taking the consideration of FRET efficiency of 68% between donor and acceptor. The sequential approach tended to lower the signal/noise (S/N ratio and the detection of the model antigen (lower than 1 ng/mL had better sensitivity than the model antibody (lower than 50 ng/mL. This combined antigen/antibody method might be useful for combined detection of antigens and antibodies. It will be helpful to screen for both antigen and antibody particularly in the situations of the multiserotype and high-frequency mutant virus infections.

  9. Evaluation of HIV antigen /antibody combination ELISAs for ...

    African Journals Online (AJOL)

    Abstract. Introduction: the aim of this study was to evaluate the performance of Enzygnost HIV Integral II antigen/antibody combination ELISAs in order to formulate HIV ELISA testing algorithms for the Ministry of Health and Social Welfare, Tanzania. Methods: this was a laboratory-based evaluation of Enzygnost HIV Integral ...

  10. Antinuclear antibody testing in obstetric patients | Afman | South ...

    African Journals Online (AJOL)

    Objectives. To assess possible associations between the presence of antinuclear antibodies (ANAs) and pregnancy outcome in order to determine the significance of this test in obstetric practice. Methods. A case-control study was performed on 408 patients admitted to an obstetric high care unit and on whom ANA testing ...

  11. Anti-Ro52 antibody level is an important marker of fetal congenital heart block risk in anti-Ro/SSA antibody positive pregnancy.

    Science.gov (United States)

    Miyasato-Isoda, Mai; Waguri, Masako; Yamada, Yuko; Miyano, Akira; Wada, Yoshinao

    2017-09-21

    The aims of this study are to determine the incidence of congenital heart block (CHB) in the Japanese population and identify maternal factors predicting fetal CHB in anti-Ro/SSA antibody positive pregnancy. A retrospective study was performed using 52,147 clinical records of pregnancies followed in a single center. For 183 anti-Ro/SSA antibody-positive women, anti-Ro52 and Ro60 antibodies were measured, and the odds of CHB in relation to maternal clinical features were calculated by multivariate analysis. The receiver-operating characteristic (ROC) curves for predicting CHB were constructed for the titers of anti-Ro/SSA, anti-Ro52 and anti-Ro60 antibodies. Fetal CHB occurred in two pregnancies among those without known risks such as positive anti-Ro/SSA antibody or previous CHB-affected pregnancy, suggesting an incidence similar to that in Caucasian populations. As for the anti-Ro/SSA antibody positive pregnancies, the titers of anti-Ro/SSA, anti-Ro52 and anti-Ro60 antibodies were independent risk factors for fetal CHB and the use of corticosteroids before 18 gestational weeks was an independent protective factor. The area under the ROC was 0.84, 0.73 and 0.74 for anti-Ro52, anti-Ro60 and anti-Ro/SSA antibodies, respectively. CHB occurred in two among approximately 50,000 pregnancies without known risks such as positive anti-Ro/SSA antibody or previous delivery of CHB-affected babies. Measurement of anti-Ro52 antibody levels may be helpful in extracting a risk group of delivering CHB infants in the anti-Ro/SSA antibody positive pregnancy.

  12. Radioimmunological determination of growth hormone antibodies

    International Nuclear Information System (INIS)

    Kracmar, P.; Hnikova, O.

    1979-01-01

    The method is based on the assumption of the presence of antibodies in the serum of the patient and the formation of the complex antibody-tracer ( 125 I-STH). For separation the principle is used of two antibodies and subsequent ultrafiltration with membrane ultrafilters. Clinical experience, reproducibility and the procedure recommended for simple monitoring and the determination of the amount of antibodies in the serum of patients are presented. (author)

  13. Antibody therapeutics - the evolving patent landscape.

    Science.gov (United States)

    Petering, Jenny; McManamny, Patrick; Honeyman, Jane

    2011-09-01

    The antibody patent landscape has evolved dramatically over the past 30 years, particularly in areas of technology relating to antibody modification to reduce immunogenicity in humans or improve antibody function. In some cases antibody techniques that were developed in the 1980s are still the subject of patent protection in the United States or Canada. Copyright © 2011 Elsevier B.V. All rights reserved.

  14. Antibodies against chromosomal beta-lactamase

    DEFF Research Database (Denmark)

    Giwercman, B; Rasmussen, J W; Ciofu, Oana

    1994-01-01

    A murine monoclonal anti-chromosomal beta-lactamase antibody was developed and an immunoblotting technique was used to study the presence of serum and sputum antibodies against Pseudomonas aeruginosa chromosomal group 1 beta-lactamase in patients with cystic fibrosis (CF). The serum antibody resp...... against the infection. On the other hand, immune complexes between the beta-lactamase and corresponding antibodies could play a role in the pathogenesis of bronchopulmonary injury in CF by mediating hyperimmune reactions....

  15. [Monoclonal antibodies against PCSK9: from bench to clinic].

    Science.gov (United States)

    Guijarro Herraiz, Carlos

    2016-05-01

    Antibodies are glycoproteins with high specificity binding to multiple antigens due to the large number of structural conformations of the variable chains. Hybridoma technology (fusion of myeloma cells with immunoglobulin-producing lymphocytes) has allowed the synthesis of large quantities of unique antibodies (monoclonal [mAb]). mAbs were initially murine. Subsequently, chimeric mAbs were developed, followed by humanized mAbs and finally human mAbs. The high selectivity and good tolerance of human mAbs allows their therapeutic administration to block specific exogenous or endogenous molecules. Selective human mAbs to the catalytic domain of PCSK9 have recently been developed. These antibodies block PCSK9, favour low-density lipoprotein receptor recycling and markedly reduce circulating cholesterol. Preliminary studies indicate that lowering cholesterol through anti-PCSK9 antibodies may significantly reduce the cardiovascular complications of arteriosclerosis. Copyright © 2016 Elsevier España, S.L.U. y Sociedad Española de Arteriosclerosis. All rights reserved.

  16. Production and purification of polyclonal antibody against melatonin hormone

    Directory of Open Access Journals (Sweden)

    Fooladsaz K

    1999-09-01

    Full Text Available Nowadays immunochemical techniques have played a very important and valuable role in quantitative and qualitative assays of liquid compounds of the body. Producing antibody against immunogenes is the first step to make immunochemical kits. In this study production and purification of polyclonal antibody against melatonin has been considered. This hormone which has several important functions in physiological conditions such as migraine, cirrhosis, mammary gland cancer and other diseases, is the most important pineal gland secretion. This gland is a circumventricular organ of brain and according to histological and anatomical studies, it is a high secretory organ, that secretes active biological substances like melatonin, oxytocin, serotonin and ect. In this study, melatonin has been considered as hapten and has become an immunogen by being linked to the bovine serum Albumin. Then, by the immunization of three white New Zeland rabbits that had the booster injections in regular intervals, the antibody titer was detected to be 1/2000, by using checkboard curves, and with the use of melatonin linked to penicillinase as a labeled antigen, the titer was detected 1/200. Finally an antibody with high purification rate has been obtained, which can be used in immunochemical assays like RIA, ELISA, and EIA.

  17. Radioimmunoassay method for detection of gonorrhea antibodies

    International Nuclear Information System (INIS)

    1975-01-01

    A novel radioimmunoassay for the detection of gonorrhea antibodies in serum is described. A radionuclide is bound to gonorrhea antigens produced by a growth culture. In the presence of gonorrhea antibodies in the serum, an antigen-antibody conjugate is formed, the concentration of which can be measured with conventional radiometric methods. The radioimmunoassay is highly specific

  18. Antibodies Against Melanin | Wassermann | South African Medical ...

    African Journals Online (AJOL)

    This study reports on unsuccessful attempts to produce antibodies against melanoprotein in rabbits. Available evidence suggests antibodies against melanocytes in the aetiology of vitiligo, but there is no convincing evidence for antibodies against melanin per se. It is suggested that the demonstration of antibodif's against ...

  19. Dual-Color Bioluminescent Sensor Proteins for Therapeutic Drug Monitoring of Antitumor Antibodies.

    Science.gov (United States)

    van Rosmalen, Martijn; Ni, Yan; Vervoort, Daan F M; Arts, Remco; Ludwig, Susann K J; Merkx, Maarten

    2018-03-06

    Monitoring the levels of therapeutic antibodies in individual patients would allow patient-specific dose optimization, with the potential for major therapeutic and financial benefits. Our group recently developed a new platform of bioluminescent sensor proteins (LUMABS; LUMinescent AntiBody Sensor) that allow antibody detection directly in blood plasma. In this study, we targeted four clinically important therapeutic antibodies, the Her2-receptor targeting trastuzumab, the anti-CD20 antibodies rituximab and obinutuzumab, and the EGFR-blocking cetuximab. A strong correlation was found between the affinity of the antibody binding peptide and sensor performance. LUMABS sensors with physiologically relevant affinities and decent sensor responses were obtained for trastuzumab and cetuximab using mimotope and meditope peptides, respectively, with affinities in the 10 -7 M range. The lower affinity of the CD20-derived cyclic peptide employed in the anti-CD20 LUMABS sensor ( K d = 10 -5 M), translated in a LUMABS sensor with a strongly attenuated sensor response. The trastuzumab and cetuximab sensors were further characterized with respect to binding kinetics and their performance in undiluted blood plasma. For both antibodies, LUMABS-based detection directly in plasma compared well to the analytical performance of commercial ELISA kits. Besides identifying important design parameters for the development of new LUMABS sensors, this work demonstrates the potential of the LUMABS platform for point-of-care detection of therapeutic antibodies.

  20. Identification and evaluation of nextgeneration PTM-specific antibodies

    DEFF Research Database (Denmark)

    Persson, Nina Emilia

    selectionprocess needs to exclude cross-reactive or suboptimal binders at an early stage to avoid problemsin the downstream evaluation process.In this thesis, a microarray screening assay is described. This assay combines an antibody and anantigen microarray for high content screening of multiple single......-chain fragment variable (scFv)clones. Two different analyses are performed on the same microarray. There is no need for anypurification or enrichment before screening. In the first analysis, the ability of the individualscFv clone to bind to the soluble form of the antigens is evaluated. Favouring selection.......In subsequent project, a new antibody library was constructed from the heavy chain variabledomain of G2-D11 and random light chains domains from immunized mice. Using this antibodylibrary and our screening assay, we could select a number of antibodies specific for the Tnantigenon a peptide from the CD43 protein...

  1. Detection of IgE insulin antibody with radioallergosorbent test

    International Nuclear Information System (INIS)

    Nakagawa, S.; Nakayama, H.; Sasaki, T.; Watanabe, T.; Aoki, S.; Saito, N.

    1978-01-01

    An in vitro method for detecting IgE insulin antibody using the principle of the radioallergosorbent test (RAST) is described. In six patients with insulin allergy, the RAST values were higher than in normal persons or insulin-treated diabetics without insulin allergy. No differences were observed between normal persons and insulin-treated diabetics without insulin allergy. Moreover, it was observed that in one patient treated with highly purified insulin, there was a gradual decrease of RAST value parallel to the radioinsulin binding activity and clinical allergic symptoms. The RAST value of insulin is slightly inhibited by non-IgE antibodies and is, therefore, a semiquantitative value. However, the RAST is simple to perform and reproducible; it is therefore very useful in the detection of IgE insulin antibodies. (orig.) [de

  2. Immunoscintigraphy of ovarian carcinoma using OC 125 monoclonal antibody

    International Nuclear Information System (INIS)

    Park, Sang Yoon

    1990-03-01

    Immunoscintigraphy (ISG) with I-131 labeled OC 125 F (ab')2 fragments was studied in 7 patients for primary diagnosis and follow up of ovarian cancer. Total body planar photoscans with a scintillation camera were performed three to seven days after antibody application and results were compared with operation and/or computed tomography (CT) examination. By the region of interest technique, the tumor to background ratio was calaulated in vivo. Results are as follows. 1) The sensitivity of ISG and CT for detection of 14 tumor sites which were confirmed with histopathology were 100 % and 57.1 % and the sensitivity for the detection of omental metastasis were 100 % and 20 % respectively. 2) There were no correlation between the serum CA 125 levels and tumor to background antibody uptake ratio. 3) Tumor to background antibody uptake ratio were progressively increased from day 3 to day 7. (author)

  3. Characterization of fully functional spray-on antibody thin films

    Energy Technology Data Exchange (ETDEWEB)

    Figueroa, Jhon [Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620-5250 (United States); Magaña, Sonia; Lim, Daniel V. [Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620-7115 (United States); Schlaf, Rudy, E-mail: schlaf@eng.usf.edu [Department of Electrical Engineering, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620-5101 (United States)

    2014-02-15

    The authors recently demonstrated that fully functional Escherichia coli O157:H7 antibody thin films can be prepared using a simple pneumatic nebulizer on glass surface [1]. This paper focuses on the investigation of the morphology and physical properties of these films with the aim to better understand their performance. A series of E. coli O157:H7 antibody spray-on thin films were investigated by ellipsometry, X-ray photoelectron spectroscopy (XPS), immunoassays, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), fluorescence microscopy, atomic force microscope (AFM) and contact angle analysis. These data were compared to measurements on films prepared with the biotin–avidin covalent bonding scheme. The investigation showed that films created by a 2 min pneumatic spray deposition time can capture antigens similar as the avidin–biotin wet-chemical method. The results also suggests that an influential factor for the comparable capture cell ability between sprayed and covalent films is an increased antibody surface coverage for the sprayed films (non-equilibrium technique), which compensates for the lack of its antibody orientation. There was no significant antibody denaturation detected on any of the sprayed films. Both techniques led to the formation of cluster-aggregates, a factor that seems unavoidable due to the natural tendency of protein to cluster. The avidin–biotin bridge films generally had a higher roughness, which manifested itself in a higher wettability compared to the sprayed films.

  4. Novel Antibody-Based Proteins for Cancer Immunotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Fuenmayor, Jaheli; Montaño, Ramon F., E-mail: jfuenmay@ivic.gob.ve [Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas. Caracas, 1020-A (Venezuela, Bolivarian Republic of)

    2011-08-19

    The relative success of monoclonal antibodies in cancer immunotherapy and the vast manipulation potential of recombinant antibody technology have encouraged the development of novel antibody-based antitumor proteins. Many insightful reagents have been produced, mainly guided by studies on the mechanisms of action associated with complete and durable remissions, results from experimental animal models, and our current knowledge of the human immune system. Strikingly, only a small percent of these new reagents has demonstrated clinical value. Tumor burden, immune evasion, physiological resemblance, and cell plasticity are among the challenges that cancer therapy faces, and a number of antibody-based proteins are already available to deal with many of them. Some of these novel reagents have been shown to specifically increase apoptosis/cell death of tumor cells, recruit and activate immune effectors, and reveal synergistic effects not previously envisioned. In this review, we look into different approaches that have been followed during the past few years to produce these biologics and analyze their relative success, mainly in terms of their clinical performance. The use of antibody-based antitumor proteins, in combination with standard or novel therapies, is showing significant improvements in objective responses, suggesting that these reagents will become important components of the antineoplastic protocols of the future.

  5. Characterization of fully functional spray-on antibody thin films

    Science.gov (United States)

    Figueroa, Jhon; Magaña, Sonia; Lim, Daniel V.; Schlaf, Rudy

    2014-02-01

    The authors recently demonstrated that fully functional Escherichia coli O157:H7 antibody thin films can be prepared using a simple pneumatic nebulizer on glass surface [1]. This paper focuses on the investigation of the morphology and physical properties of these films with the aim to better understand their performance. A series of E. coli O157:H7 antibody spray-on thin films were investigated by ellipsometry, X-ray photoelectron spectroscopy (XPS), immunoassays, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), fluorescence microscopy, atomic force microscope (AFM) and contact angle analysis. These data were compared to measurements on films prepared with the biotin-avidin covalent bonding scheme. The investigation showed that films created by a 2 min pneumatic spray deposition time can capture antigens similar as the avidin-biotin wet-chemical method. The results also suggests that an influential factor for the comparable capture cell ability between sprayed and covalent films is an increased antibody surface coverage for the sprayed films (non-equilibrium technique), which compensates for the lack of its antibody orientation. There was no significant antibody denaturation detected on any of the sprayed films. Both techniques led to the formation of cluster-aggregates, a factor that seems unavoidable due to the natural tendency of protein to cluster. The avidin-biotin bridge films generally had a higher roughness, which manifested itself in a higher wettability compared to the sprayed films.

  6. Effect of the rally length on performance according to the final action and the playing level in high-level men’s volleyball. [Efecto de la duración de la jugada sobre el rendimiento en función de la acción final y del nivel de juego en voleibol masculino de alto nivel].

    Directory of Open Access Journals (Sweden)

    Joaquín Sánchez-Moreno

    2018-04-01

    Full Text Available Abstract The aim of this study was to analyze how rally length affected performance according to the final action of the rally and the playing level, as well as to identify potential critical rallies associated with rally length in high-level men’s volleyball. Thirty-one matches (5,438 rallies of the top ranking national teams were sampled from two of the premier worldwide competitions: Men’s World Championship and Men’s World League. Rallies between eight and ten seconds emerged as critical incidents of the game, changing the general trend in performance according to the final action of the rally (attack point or attack error with or without opposite team’s contact. Rallies longer than ten seconds seemed to balance the chances of success between both teams, with the team in the side-out phase losing the initial advantage of being the first team to attack. Differences were found among teams of similar level, suggesting that the ability to efficiently manage some game situations might be attributed to team’s features. Coaches may deliver drills with varying playing styles and strategies depending on the length of the really, determining the degree of risk according to the length. Resumen El objetivo de este estudio fue analizar cómo la duración de la jugada afecta al rendimiento de los equipos en función de la acción final y del nivel de juego, así como identificar las jugadas potencialmente críticas asociadas a su duración en voleibol masculino de alto nivel. Se analizaron treinta y un partidos (5438 jugadas, donde se enfrentaban las mejores selecciones nacionales clasificadas en dos de las mejores competiciones mundiales: el Campeonato del Mundo Masculino y la Liga Mundial Masculina. Las jugadas de entre ocho y diez segundos surgieron como incidentes críticos del juego alterando la tendencia general esperada respecto al rendimiento de los equipos en función de la acción final ocurrida (puntos o errores de ataque, directos o

  7. Studying host cell protein interactions with monoclonal antibodies using high throughput protein A chromatography.

    Science.gov (United States)

    Sisodiya, Vikram N; Lequieu, Joshua; Rodriguez, Maricel; McDonald, Paul; Lazzareschi, Kathlyn P

    2012-10-01

    Protein A chromatography is typically used as the initial capture step in the purification of monoclonal antibodies produced in Chinese hamster ovary (CHO) cells. Although exploiting an affinity interaction for purification, the level of host cell proteins in the protein A eluent varies significantly with different feedstocks. Using a batch binding chromatography method, we performed a controlled study to assess host cell protein clearance across both MabSelect Sure and Prosep vA resins. We individually spiked 21 purified antibodies into null cell culture fluid generated with a non-producing cell line, creating mock cell culture fluids for each antibody with an identical composition of host cell proteins and antibody concentration. We demonstrated that antibody-host cell protein interactions are primarily responsible for the variable levels of host cell proteins in the protein A eluent for both resins when antibody is present. Using the additives guanidine HCl and sodium chloride, we demonstrated that antibody-host cell protein interactions may be disrupted, reducing the level of host cell proteins present after purification on both resins. The reduction in the level of host cell proteins differed between antibodies suggesting that the interaction likely varies between individual antibodies but encompasses both an electrostatic and hydrophobic component. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Validation of Antibody-Based Strategies for Diagnosis of Pediatric Celiac Disease Without Biopsy.

    Science.gov (United States)

    Wolf, Johannes; Petroff, David; Richter, Thomas; Auth, Marcus K H; Uhlig, Holm H; Laass, Martin W; Lauenstein, Peter; Krahl, Andreas; Händel, Norman; de Laffolie, Jan; Hauer, Almuthe C; Kehler, Thomas; Flemming, Gunter; Schmidt, Frank; Rodrigues, Astor; Hasenclever, Dirk; Mothes, Thomas

    2017-08-01

    A diagnosis of celiac disease is made based on clinical, genetic, serologic, and duodenal morphology features. Recent pediatric guidelines, based largely on retrospective data, propose omitting biopsy analysis for patients with concentrations of IgA against tissue transglutaminase (IgA-TTG) >10-fold the upper limit of normal (ULN) and if further criteria are met. A retrospective study concluded that measurements of IgA-TTG and total IgA, or IgA-TTG and IgG against deamidated gliadin (IgG-DGL) could identify patients with and without celiac disease. Patients were assigned to categories of no celiac disease, celiac disease, or biopsy required, based entirely on antibody assays. We aimed to validate the positive and negative predictive values (PPV and NPV) of these diagnostic procedures. We performed a prospective study of 898 children undergoing duodenal biopsy analysis to confirm or rule out celiac disease at 13 centers in Europe. We compared findings from serologic analysis with findings from biopsy analyses, follow-up data, and diagnoses made by the pediatric gastroenterologists (celiac disease, no celiac disease, or no final diagnosis). Assays to measure IgA-TTG, IgG-DGL, and endomysium antibodies were performed by blinded researchers, and tissue sections were analyzed by local and blinded reference pathologists. We validated 2 procedures for diagnosis: total-IgA and IgA-TTG (the TTG-IgA procedure), as well as IgG-DGL with IgA-TTG (TTG-DGL procedure). Patients were assigned to categories of no celiac disease if all assays found antibody concentrations celiac disease if at least 1 assay measured antibody concentrations >10-fold the ULN. All other cases were considered to require biopsy analysis. ULN values were calculated using the cutoff levels suggested by the test kit manufacturers. HLA typing was performed for 449 participants. We used models that considered how specificity values change with prevalence to extrapolate the PPV and NPV to populations with lower

  9. Solid phase double-antibody radioimmunoassay procedure

    International Nuclear Information System (INIS)

    Niswender, G.D.

    1977-01-01

    The present invention is concerned with the radioimmunoassay (RIA) procedure for assaying body fluid content of an antigenic substance which may either be an antigen itself or a hapten capable of being converted, such as by means of reaction with a protein, to an antigenic material. The present invention is concerned with a novel and improved modification of a double-antibody RIA technique in which there is a first antibody that is specific to the antigenic substance suspected to be present in a body fluid from which the assay is intended. The second antibody, however, is not specific to the antigenic substance or analyte, but is an antibody against the first antibody

  10. Production of Monoclonal Antibody against Human Nestin

    OpenAIRE

    Hadavi, Reza; Zarnani, Amir Hassan; Ahmadvand, Negah; Mahmoudi, Ahmad Reza; Bayat, Ali Ahmad; Mahmoudian, Jafar; Sadeghi, Mohammad-Reza; Soltanghoraee, Haleh; Akhondi, Mohammad Mehdi; Tarahomi, Majid; Jeddi-Tehrani, Mahmood; Rabbani, Hodjattallah

    2010-01-01

    We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140?250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such a...

  11. Antibody pretargeting advances cancer radioimmunodetection and radioimmunotherapy.

    Science.gov (United States)

    Goldenberg, David M; Sharkey, Robert M; Paganelli, Giovanni; Barbet, Jacques; Chatal, Jean-François

    2006-02-10

    This article reviews the methods of pretargeting, which involve separating the targeting antibody from the subsequent delivery of an imaging or therapeutic agent that binds to the tumor-localized antibody. This provides enhanced tumor:background ratios and the delivery of a higher therapeutic dose than when antibodies are directly conjugated with radionuclides, as currently practiced in cancer radioimmunotherapy. We describe initial promising clinical results using streptavidin-antibody constructs with biotin-radionuclide conjugates in the treatment of patients with malignant gliomas, and of bispecific antibodies with hapten-radionuclides in the therapy of tumors expressing carcinoembryonic antigen, such as medullary thyroid and small-cell lung cancers.

  12. Serum Antibody Biomarkers for ASD

    Science.gov (United States)

    2015-10-01

    45-56. Singh VK. (2009) Phenotypic expression of autoimmune autistic disorder (AAD): A major subset of autism. Ann Clin Psychiat. 21:148-160. 5...spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impairments in communication (verbal and nonverbal), social interactions, and... autoimmunity ; in particular, the generation of antibodies reactive against brain and CNS proteins. The goal of this grant is to identify serum

  13. Antibody Repertoire Development in Swine

    Czech Academy of Sciences Publication Activity Database

    Butler, J. E.; Wertz, N.; Šinkora, Marek

    2017-01-01

    Roč. 5, FEB 17 (2017), s. 255-279 ISSN 2165-8102 R&D Projects: GA ČR GA15-02274S; GA ČR(CZ) GA16-09296S Institutional support: RVO:61388971 Keywords : swine * pre-immune antibody repertoire * ileal Peyer's patches Subject RIV: EE - Microbiology, Virology OBOR OECD: Microbiology Impact factor: 4.708, year: 2016

  14. Determining the limit of detection of surface bound antibody.

    Science.gov (United States)

    Madiona, Robert M T; Welch, Nicholas G; Scoble, Judith A; Muir, Benjamin W; Pigram, Paul J

    2017-08-11

    Determination of a limit of detection (LoD) for surface bound antibodies is crucial for the development and deployment of sensitive bioassays. The measurement of very low concentrations of surface bound antibodies is also important in the manufacturing of pharmaceutical products such as antibody-conjugated pharmaceuticals. Low concentrations are required to avoid an immune response from the target host. Enzyme-linked immunosorbent assay (ELISA), x-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF-SIMS) were used to determine the LoD for the surface bound antibody (antiepidermal growth factor receptor antibody) on silicon substrates. Antibody solution concentrations between 10 μg/ml and 1 ng/ml and a control (antibody-free buffer solution) were employed, and the detection performance of each technique was compared. For this system, the ELISA LoD was 100 ng/ml and the XPS LoD was 1 μg/ml, corresponding to an estimated surface concentration of 49  ± 7 ng/cm 2 using a 1 μg/ml solution. Due to the multivariate complexity of ToF-SIMS data, analysis was carried out using three different methods, peak ratio calculations, principal component analysis, and artificial neural network analysis. The use of multivariate analysis with this dataset offers an unbiased analytical approach based on the peaks selected from ToF-SIMS data. The results estimate a ToF-SIMS LoD between applied antibody concentrations of 10 and 100 ng/mL. For surface bound antibodies on a silicon substrate, the LoD is below an estimated surface concentration of 49 ng/cm 2 . The authors have determined the LoD for this system using ELISA, XPS, and ToF-SIMS with multivariate analyses, with ToF-SIMS offering an order of magnitude better detection over ELISA and 2 orders of magnitude better detection over XPS.

  15. Association analysis of anti-Epstein-Barr nuclear antigen-1 antibodies, anti-cyclic citrullinated peptide antibodies, the shared epitope and smoking status in Brazilian patients with rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Michel Alexandre Yazbek

    2011-01-01

    Full Text Available INTRODUCTION: Epstein-Barr virus exposure appears to be an environmental trigger for rheumatoid arthritis that interacts with other risk factors. Relationships among anti-cyclic citrullinated peptide antibodies, the shared epitope, and smoking status have been observed in patients with rheumatoid arthritis from different populations. OBJECTIVE: To perform an association analysis of anti-Epstein-Barr nuclear antigen-1 antibodies, anti-cyclic citrullinated peptide antibodies, the shared epitope, and smoking status in Brazilian patients with rheumatoid arthritis. METHODS: In a case-control study, 140 rheumatoid arthritis patients and 143 healthy volunteers who were matched for age, sex, and ethnicity were recruited. Anti-Epstein-Barr nuclear antigen-1 antibodies and anti-cyclic citrullinated peptide antibodies were examined using an enzyme-linked immunosorbent assay, and shared epitope alleles were identified by genotyping. Smoking information was collected from all subjects. A comparative analysis of anti-Epstein-Barr nuclear antigen-1 antibodies, anti-cyclic citrullinated peptide antibodies, the shared epitope, and smoking status was performed in the patient group. Logistic regression analysis models were used to analyze the risk of rheumatoid arthritis. RESULTS: Anti-Epstein-Barr nuclear antigen-1 antibodies were not associated with anti-cyclic citrullinated peptide antibodies, shared epitope alleles, or smoking status. Anti-cyclic citrullinated peptide antibody positivity was significantly higher in smoking patients with shared epitope alleles (OR = 3.82. In a multivariate logistic regression analysis using stepwise selection, only anti-cyclic citrullinated peptide antibodies were found to be independently associated with rheumatoid arthritis (OR = 247.9. CONCLUSION: Anti-Epstein-Barr nuclear antigen-1 antibodies did not increase the risk of rheumatoid arthritis and were not associated with the rheumatoid arthritis risk factors studied. Smoking

  16. Performance limits of a helium-cooled divertor (unconventional design). Final report on subtask PPA1.3.2 of the preparation of a power plant conceptual study on plant availability

    International Nuclear Information System (INIS)

    Kleefeldt, K.; Gordeev, S.

    2000-03-01

    In the frame of preparatory work for an European power plant conceptual study to be launched in 2000, the potential of different blanket and divertor cooling concepts has been assessed with view to their performance limits in a commercial reactor. As part of this activity the present work covers investigations related to the performance of a helium-cooled divertor, employing refractory materials as structure, able to extract high-grade heat for power conversion. Performance limits in terms of maximum achievable heat flux at divertor target plates are set mainly by materials temperature and stress constraints, but also by general design requirements like pumping power and tolerable deformation. In this context a high structure-to-coolant heat transfer is essential, which the porous media (PM) heat exchanger concept promises to provide effectively. Hence, the PM is the key design feature adopted in this study. Other basic elements of the assessment are assumptions on divertor configuration and operating conditions (dimensions, cooling scheme, power division to sub-components), material data base review for refractory materials involved, and a review of alternative heat transfer enhancement methods. The main part of the study covers the analysis methods and results of the proposed concept, which are grouped in overall divertor cooling parameters, thermal-hydraulics of target plate, and thermomechanical aspects of a single target plate cooling channel. Finally, the maximum tolerable heat flux is assessed based on limits set by temperature windows, thermal stresses and deformations. It is shown that heat fluxes of 5.5 to 6 MW/m 2 can be handled with helium-cooled concepts of unconventional design, given that the operating temperature in the only structural material deemed viable (molybdenum and tungsten alloys) must not fall below 600-700 C for reasons of embrittlement. The potential for further improvements is judged to be marginal. Coolant parameters can be kept in an

  17. Impact of Stainless Steel Exposure on the Oxidation of Polysorbate 80 in Histidine Placebo and Active Monoclonal Antibody Formulation.

    Science.gov (United States)

    Gopalrathnam, Ganapathy; Sharma, Anant Navanithan; Dodd, Steven Witt; Huang, Lihua

    2018-01-01

    Rapid oxidation of polysorbate 80 in histidine buffer was observed upon brief exposure to stainless steel. Liquid chromatography-mass spectrometry analysis indicates degradation of both polyoxyethylene sorbitan and polyoxyethylene head groups and unsaturated fatty acid chains, with further confirmation by reversed-phase high-performance liquid chromatography data. Both Fe 2+ and Fe 3+ were shown to induce polysorbate 80 oxidation. The degree of oxidation in polysorbate 20 and polysorbate 80 are comparable for the head groups and saturated fatty acid esters. However, the same phenomenon was not observed with placebo or monoclonal antibody at a threshold protein concentration, formulated in sodium citrate, in combination with histidine and sodium citrate, or with Na 2 ethylenediaminetetraacetic acid (EDTA). Further, polysorbate 80 oxidation was not observed with Lilly's antibody containing the active ingredient LY2951742, at or above a threshold concentration. Finally, no major polysorbate 80 degradation was observed in histidine buffer, with or without protein, in containers composed of glass or plastic, or when stainless steel exposure was otherwise completely absent. Finally, the 2-oxo oxidation form of histidine was not observed, but the other oxidation products and modifications of histidine were identified. LAY ABSTRACT: Rapid oxidation of polysorbate 80 in histidine buffer was observed upon brief exposure to stainless steel. The degree of oxidation in polysorbate 80 and polysorbate 20 were comparable. However, the same phenomenon was not observed with placebo when formulated in sodium citrate, in combination with histidine and sodium citrate, or with Na 2 ethylenediaminetetraacetic acid (EDTA). Polysorbate 80 oxidation was not observed with Lilly's antibody containing the active ingredient, LY2951742, at or above a threshold concentration. No major polysorbate 80 degradation in histidine buffer was observed when stainless steel contact was completely absent.

  18. Njv Magazine 3 final

    African Journals Online (AJOL)

    En-Joy

    methods (wet blood mounts, Giemsa stained thin/thick blood smears, and microhematocrit centrifugation technique) can be used to confirm diagnosis in infected goats. Inoculation of laboratory rodents with suspected blood may be indicated in the diagnosis of some trypanosome infections. Immuno- fluorescent antibody ...

  19. Njv Magazine 3 final

    African Journals Online (AJOL)

    En-Joy

    ABSTRACT. Newcastle disease affects different age group of birds despite vaccination from day old. In order to determine the influence of maternally derived antibody (MDA) on chicks in Newcastle disease (ND) vaccination, a total of 100 broiler chicks were divided into four equal groups, A, B, C and. D. Different ND ...

  20. The Skills Enhancement Literacy Project of Hawaii. Final Program Model. Final Performance Report. Final Evaluation Report.

    Science.gov (United States)

    Hawaii Univ., Manoa. Coll. of Education.

    The Skills Enhancement Literacy Project of Hawaii (SELPH) was a demonstration workplace literacy partnership between the College of Education, University of Hawaii-Manoa and the ITT Sheraton Hotels. Four Sheraton Hotels in Waikiki participated in the project. The program was planned, staff and volunteers were recruited, and marketing strategies…